LOCUS       DAOQNZ010000001        11626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_1972_length_11626_cov_3.320111, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000001 DAOQNZ010000000
VERSION     DAOQNZ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1972_length_11626_cov_3.320111"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKW73_00005"
     CDS             <1..914
                     /locus_tag="PKW73_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71689.1"
                     /translation="PLELLRNSGALNVLGAENYFYDYKEALLDVNEKLLTTVCEGCSV
                     GLKGNKSVKDDGPKDCRLRSALLMDNSRLAKILKNRMDRSQKMRTGQYEAIKRDITRL
                     LPVKAEEDIPDALKDTPIATLLKCQNLGQVESSLSDSPELIIGMCIDYRKSLTLPQNC
                     AYVIRRTGANMAGSEFSLALALSAGIKYMALIAHNHCIMSDPHTKREDFVGALTTHHG
                     WTEAEAKRFFDKHASSREISDAIDFSVKEARRLTRLFNGARIVPMMFMLEDNMLYLVK
                     DWVEEELGKDPLVIVEEIEEASAASQN"
     gene            1094..2287
                     /locus_tag="PKW73_00010"
     CDS             1094..2287
                     /locus_tag="PKW73_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71690.1"
                     /translation="MSGAIKKRVGVATIIVLAAAMLVVSAIFIKPREKVATLVNADKL
                     MAGASTSGVWDTRFADKVMKTVFFALVLTSDEYTQQNAEALEALRSWAAREFHRDPRF
                     LHPVILPAPVANSTFVNSSVYSLLASPYYGLRSLSGDFLSWRMEQFSQSRAFHTFTKN
                     CFEFFGEEPEAAAMLARYQSDRAFAAIDSMLAFCYFLLALLISRIPVRFLKEKAAYWR
                     APLGQLESAGIAAELAYGTFVVAGFYFLQSVIQEALAGQSLLACLLSIGLGLYVLFPV
                     KIFADRETVFCLRSSLTVRTVAACAFLFLSLLVVQLLNWLKQGSLTAPDPLTLIISGL
                     TGDFIHEPSQSKKTLALVISSLWALALAFTLKTVTRRAGVSSAEVAKRLSESDGLKSS
                     STLKG"
     gene            2315..2398
                     /locus_tag="PKW73_00015"
     tRNA            2315..2398
                     /locus_tag="PKW73_00015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2349..2351,aa:Leu,seq:taa)
     gene            complement(2433..3299)
                     /locus_tag="PKW73_00020"
     CDS             complement(2433..3299)
                     /locus_tag="PKW73_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNA71691.1"
                     /translation="MLYKNFSHEVEIFGQKLPEPRVLLDFLGFETHIKQIRSKRGADV
                     WPGWYDHAAYYIVDLPADKVYGPGDEVPLPDCVLAPDYEFEIGCLATREALITSEAEG
                     LKYFQEHCYLTILNDWSARDIQMKDIQGLGPANSKFVMGKSIGPRFVQAGSLPMDDNG
                     VLFAEMTLKVNGQVRSQSNYQSLYHTHPVTQAKAAWSFPRIFAWLGRQNIAIHPGYLF
                     GSGTVGNGCIAEFSAQIDPTTGAELAPAKYGWLKDGDEITMEVEGLGSLSNKVRAMAN
                     NDSKTQQKTAVL"
     gene            3460..3831
                     /locus_tag="PKW73_00025"
     CDS             3460..3831
                     /locus_tag="PKW73_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNA71692.1"
                     /translation="MKHSEAFEKIVAEVKPRIKEVTIDDVLSWQAAQEDFVFMDVRED
                     HEWREGRACGSIHLGRGILERDIEKVVEDKGKKVVLYCGGGYRSALAADNMQKMGYTN
                     VLSMAGGIRAWRERNLPEERD"
     gene            3831..4166
                     /locus_tag="PKW73_00030"
     CDS             3831..4166
                     /locus_tag="PKW73_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71693.1"
                     /translation="MVAPVVRSKVLFCSLAASFLWCRANVVWASAVEDYLYEFTQMQK
                     TDPNGFYLMVAGVSLMAFLALTIFAGFMFYLYKGWGAEQAEDARHRAAAQARAGAKAT
                     DKEGPPAAS"
     gene            4301..4732
                     /locus_tag="PKW73_00035"
     CDS             4301..4732
                     /locus_tag="PKW73_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71694.1"
                     /translation="MAQTRKNKKLLAPAQDTLSLDGLESFLENATLETVKQDETIDFS
                     PSVTAEPPLSETRLILDLLAQLENVNDLVLETGYQLAQTRERVRELEGLLMGEELLVA
                     RIKDLEVEAQKVLELETWLDALRAENEHLKRPWWKKLLGLN"
     gene            4871..5401
                     /locus_tag="PKW73_00040"
     CDS             4871..5401
                     /locus_tag="PKW73_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71695.1"
                     /translation="MANFDEPQKVKPELETERSPLNLADLSINMPGSISHGRDLVSGS
                     DAGKALPGLMLVENAGAETHGDGRRVPNADGSEKFNFLPTEDPDSKWYLDGLAKPGKY
                     LGDSETLHDKEFVAGGGKAPDAGAPAHEPGGSDYQDNGAVRPGKVDSADSDSPWYSDG
                     TASGKYRFGDRNTALD"
     gene            complement(5403..6791)
                     /locus_tag="PKW73_00045"
     CDS             complement(5403..6791)
                     /locus_tag="PKW73_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNA71696.1"
                     /translation="MFLKQFYLGCLAHASYVLGSGQEACVIDPQRDVEEYLTELQSQG
                     LKLKYIIETHLHADFVSGHNELAQKTGAEIVFSHKARVELPHIAARDGQKLSLGDLTL
                     TILETPGHTPESICVLAEDAMSSKKMLFTGDTLFVGDVGRPDLVCQYGYEPEEMAGLL
                     YDSLHKKIMPLDDAVEVYPAHGAGSLCGKNISQERSSTLGEQRKFNWALKSMDKDSFI
                     ESLTRELPEIPKYFGLAVQTNRQGARPLAELPPLQTMTSETVLAKQKEGALLLDVRSP
                     AEFCQGHPAGALNIGLGGQFASFSGILVDADREKIIIGTKEQAQEAVVRLARAGLENV
                     SGYLEDFRTGFTVVSVPQVHVEELAERIARDPSIKVLDVRRKAEYVAGHVPGARSIPL
                     SELAAAVSDLDRDTTLHVICAGGYRSTMAVSLLLQHGFEKLENIEGGTMAWRKAGLDV
                     HVDKPSACSAGS"
     gene            6938..7723
                     /locus_tag="PKW73_00050"
     CDS             6938..7723
                     /locus_tag="PKW73_00050"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71697.1"
                     /translation="MASKNGFLPPPLPSGFFTSCCLALLTSWSSACLALGGDNHEEAS
                     RSEIESGVRLPVNVLPERYDLYFEPDFEKGDFEGSETILVDVKAPTSTISLNEQALSV
                     YDASISPKGSQGGFVKADIVRQDDKQLVLLKFPSPLSKGKHELSIKFSGRLGSKLAGF
                     YLSTFKDKQGREKKIATTQMEPTDARSMFPCFDEPAFKARYRLSVCIPKEMTAVSNSA
                     VEFEKEDKRKDKKVLSFKTTPPYSTYLFALAIGDFKEVGRVGK"
     gene            7741..9705
                     /locus_tag="PKW73_00055"
     CDS             7741..9705
                     /locus_tag="PKW73_00055"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNA71698.1"
                     /translation="MYAVNGKEHLTAYALDVAARLLPYYESYFGAEYPLDKLDLIAIP
                     DFSAGAMENPGAITFRETALLCDEKASTAMRMNVAGIIAHEMAHLWFGDLVTMKWWNG
                     LWLNEAFATWMATKAVNVLEPQWRAYDEFALARVGTLDADGLIATRPVEYPVKSPEDA
                     MEMFDEITYEKGGSLLKMLETYLGENTFQEGIKLYMKEHAFANATTGDLWRALSASAA
                     KAGKKVDVGAMMNSWVFTAGCPLLDFSLEPVRSGKKEMGKTYGVKLSQRRYLREKVSL
                     KGQESLWTVPLMLKGDGFSQPLLFSKKTESIKVKAESAPLFINGTGCGFFRTAFDETS
                     FAALVQSSRALPELLNCQERISLFSDLFALAASDGSSDPQSKAMNRYLTALAKLKDEE
                     DPYVLSLLIGQVQFFESFLPADEKLEKAYASFVCGQLKGHLERLGFERRKNDTELQCQ
                     LRGQLVRVLGTIGGDQQCIDFCRGLRDTYLTDEKSLDSDLLSAIVTVVAYNGDGEDYA
                     RILQLAKMGGTPESYQRNLGALTAFRGKEQFEASLAMVLGKDVRAQDVTRQLSRLLHN
                     RAAQKATWNFLESNWEKLKGKLDPKHLPDLVEAASVFNQEEDYKQLEAFINQHPLKNG
                     RRSAAKTLELVRIRIQFRKNALPTFIKALP"
     gene            9712..10491
                     /locus_tag="PKW73_00060"
     CDS             9712..10491
                     /locus_tag="PKW73_00060"
                     /inference="COORDINATES: protein motif:HMM:NF022784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HNA71699.1"
                     /translation="MTVLGRFKQRGKWGGIGLLLAFVLNFCGGSCSGAIAKPVLVFWS
                     WYHDDDLSSARGEVAYLTNRIVVNGSEMKDYPRTNRLKVRPGTRRTAVVRVEVRKLEG
                     AKSRLLARRIVSGALRSQVEALQIDFDARMNERAFYLELLSDIRQQLPQGIKLSMTAL
                     ASWCMQDNWLPGSNSLVDEVVPMFFSMGSRQRQAEDFLQEKLPLVPLGPRCIGLSMDE
                     PATIEKLSVRLRQFDRIYIFSRRPWTGTTLETSLGMLKRWI"
     gene            10540..>11626
                     /locus_tag="PKW73_00065"
     CDS             10540..>11626
                     /locus_tag="PKW73_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71700.1"
                     /translation="MAIGLCLSLNLLPVLACGPFFDDAVFYLRSHPDAPMKRFLSGSL
                     GVLEPTYARSYLIFAYRYLQDKPLTAAERASAYDLLCYRLGMANIDPRSEVSPREAGS
                     GVDVWNNARKKVKGARVREFSSWDTFRNIDGEDYISYLNCTPDAFITAAATLDKRIST
                     YGADSAEVKAWLEAQDQVFSHCSSSGYNYEKKQQNPPPPFPATLPATVPLAQRQDRFY
                     QLAASRFYAGEFEVARALFREIAADKASPWHQIAEYMVARTYIREGTLAKDVAKGQKL
                     LQQAMDILDGLMRKPEFASFSSSIEDLRHFLLIRLRRDGTFKDLSSKIVSDGLGDKFG
                     KSVGDYTFILDRYFNESGDESSETHEIE"
BASE COUNT         2602 a         3002 c         3196 g         2826 t
ORIGIN      
        1 aacccctgga actattgcgc aacagcgggg ccttgaatgt tctgggagct gagaactatt
       61 tttacgacta caaagaagcg cttctggatg tgaacgagaa acttctcacg acggtctgcg
      121 aaggctgctc ggtaggcttg aaggggaaca agagcgtcaa ggacgacggt ccgaaggatt
      181 gccgcttgcg ctctgccctg ctcatggata acagccgtct ggcaaagatt ttgaagaatc
      241 gtatggatcg gtcgcagaaa atgcgcaccg gacaatatga agccatcaag cgcgatatta
      301 ccaggctctt gcctgtgaaa gctgaggaag acattcctga tgcattgaaa gatactccca
      361 tagccactct tcttaaatgc cagaatttgg gtcaggtcga aagttctctt tccgatagtc
      421 ctgaactgat catcggtatg tgcatcgatt atcgcaagtc tctcaccctg cctcagaatt
      481 gcgcctacgt gatcaggcgc accggcgcca atatggccgg ctctgagttt tcgctggccc
      541 tggccctttc ggcaggcatc aaatatatgg ctctcatcgc ccacaaccat tgcatcatgt
      601 ccgaccctca taccaagcgt gaggatttcg tgggtgctct caccactcat cacggctgga
      661 ccgaggcaga ggcaaagcga ttcttcgata agcatgccag tagtcgtgag atttccgatg
      721 ctatcgattt ctccgtcaaa gaggcgcgcc gactgacacg gctcttcaac ggtgcgcgca
      781 tcgtgcccat gatgtttatg ctggaggaca acatgctcta cctggtcaaa gactgggtgg
      841 aagaggaatt aggcaaagat cctctggtga ttgtggaaga gatagaagag gccagcgcag
      901 cttctcaaaa ctgagccatc ttccattacg aattttgaat gaagacggga acctggaagt
      961 cgattgctga atcggaggca gtttggcaga gcccccgctg cgcattaatt gcttccaacc
     1021 ttggcttccc cttgcgctgc ttgagatcgt ctaaactaag tcctgcttag ctagttgccc
     1081 gatcaggtga gttatgtccg gtgccatcaa aaagagagtt ggagtcgcca ctatcattgt
     1141 gcttgcggcg gcaatgcttg tggtcagtgc catttttatc aaaccccggg agaaggtcgc
     1201 cactctcgtc aatgccgaca agctgatggc gggggcttct acctccggcg tctgggatac
     1261 tcgctttgcc gacaaggtaa tgaagaccgt ttttttcgcc ctggttttga ccagcgatga
     1321 atacacccag caaaatgcag aggctctgga agctctgcgt tcctgggcgg ccagggagtt
     1381 tcaccgcgat ccgcgtttcc tgcacccggt aatcttgccg gctccggtgg ccaattccac
     1441 ttttgtcaac tccagcgttt acagcttact ggcctcgccc tattacggac tgcgttcgct
     1501 cagtggtgat ttcctttcct ggcgtatgga gcagttttca caaagccgcg cctttcacac
     1561 tttcacaaaa aattgtttcg aattcttcgg cgaagagccg gaagctgcag ccatgctggc
     1621 tcgctatcag tcagaccggg cctttgcggc catcgattcg atgctggcct tttgttactt
     1681 cctgctggca ctcttgataa gccgcatccc ggtgcgcttc ttgaaagaaa aggccgctta
     1741 ctggcgtgcc ccccttggtc aactggaaag cgccggcatc gccgctgaac tggcctatgg
     1801 tactttcgtg gtggccggct tctatttcct gcaatcggtg atacaggaag ctctggccgg
     1861 ccagtcgcta ctggcttgcc tcttgagcat cggcctggga ctctatgtgc tcttccctgt
     1921 taagattttt gccgatcgag agacggtatt ttgcctgcgt tcgagcttga ctgtcaggac
     1981 cgtggccgcc tgtgcctttc tcttcctcag tttgcttgtg gttcagcttt tgaactggct
     2041 caaacagggc agcttgactg cgcccgatcc cctcactctg atcatctcag ggctcaccgg
     2101 tgacttcatt cacgaacctt cacagagtaa aaagaccctg gcgctggtga tctccagcct
     2161 ctgggctctg gctctcgcct ttacgctcaa gactgtcacc agacgcgccg gtgtcagcag
     2221 tgccgaagta gctaagaggt tgtcggaatc ggatggtctc aaaagcagct ctaccctgaa
     2281 ggggtagctg ctttagtgct aaaattaggc ttctgccctt gtgtcggaac tggtatacga
     2341 gtcgcactta aaatgcgatg cccgtaaggg attgagggtt cgaatccctc caagggcaat
     2401 tttcttagca tgcaaataaa cggcagcggc tcttagagaa ctgccgtttt ttgttgggtt
     2461 ttcgagtcgt tgttggccat ggctctgact ttattgctga ggctgccgag accttccact
     2521 tccatggtta tttcgtcgcc gtccttgagc cagccatatt tagccggcgc aagctcagcc
     2581 cccgtggtgg ggtctatttg ggcggaaaat tcggcaatgc agccattacc gacggtgccg
     2641 gaaccaaaca agtaaccggg gtggatggct atattctgcc taccgagcca ggcaaaaatg
     2701 cgaggaaaac tccaggcggc ctttgcttga gtgaccgggt gcgtatgata gagactctga
     2761 taattggact gactgcgcac ctgaccgttc accttgagag tcatttcagc aaacaaaacg
     2821 ccgttgtcgt ccatcggcag ggagccggct tgcacaaaac gaggtccgat gctcttgccc
     2881 attacgaact tgctgttggc cggtccgagc ccctgaatgt ccttcatctg aatgtctctg
     2941 gccgaccagt cattgaggat ggtcaggtag caatgttctt gaaagtattt tagaccctct
     3001 gcttcgctgg taatcaaagc ttctctggtg gccaggcagc ctatttcaaa ttcgtaatcg
     3061 ggggccagga cgcagtccgg caaaggtact tcatctcccg gtccgtagac tttatcggcc
     3121 ggcaggtcga ctatgtaata ggcggcgtgg tcataccagc cgggccagac atcggcgccg
     3181 cgtttggagc gaatttgctt tatatgagtt tcgaaaccaa gaaaatccag tagtactctt
     3241 ggttccggca gtttttgacc gaatatttct acttcatgag aaaagttctt gtaaagcatg
     3301 gcgtctccac ttgagagaat aagaaggtga ttgtcccatt tttgtacggt gtgacctcta
     3361 aatctcaaac atgccgttat aaactgcagg atgccaagga ggcaagggga tcgatccggt
     3421 taaattgaga ttagcctcgt taagtgagaa tggtgcagag tgaaacattc agaagccttt
     3481 gaaaagattg tggcggaagt gaaaccccgc attaaggaag taaccatcga tgatgtactt
     3541 tcctggcagg ccgcacagga agacttcgtt tttatggatg tgcgagaaga tcatgagtgg
     3601 cgggaaggac gtgcctgcgg ttccattcat cttggtcgcg gcatactgga gcgtgatatc
     3661 gagaaggtcg tggaagacaa aggtaagaag gtagttctct attgcggcgg cggttaccgt
     3721 tctgcactgg cggctgacaa tatgcagaag atgggctata cgaatgtact ttccatggcc
     3781 ggcggaatcc gcgcctggcg tgagcgcaat ttgccggagg aaagagatta atggtggcgc
     3841 cggtggtgcg cagcaaggtg ctcttttgtt cattggcggc gagttttctc tggtgccggg
     3901 ctaatgtcgt ctgggccagt gccgtggaag actatcttta tgaatttact cagatgcaga
     3961 agaccgaccc caatggtttt taccttatgg tagccggcgt gtctttgatg gcctttctgg
     4021 ccctgaccat cttcgccggg tttatgtttt atctgtacaa aggctggggg gctgagcagg
     4081 cagaagacgc caggcatcgg gccgcagccc aggcacgagc cggcgctaaa gcgaccgata
     4141 aagaaggtcc gcctgctgcg tcctgatcgc tttgtcaatt tttgactgat aactagccga
     4201 ttttcccagt ccgagcggtt tttgaatagt caagagttgc ccttcgtcct gccaccgccc
     4261 ataatgtcat cagtttcctg ctgctagaag gcggtgctgt atggcccaaa ctcgcaagaa
     4321 taaaaagctc cttgctcccg cgcaagatac tctcagcctt gatgggttgg agtcgttttt
     4381 agaaaatgcc actctggaaa ccgtcaagca ggatgagaca attgatttct ctccttccgt
     4441 cactgcggag ccgcctctaa gcgaaaccag gctgattctc gatctgctcg ctcagctcga
     4501 aaatgtaaac gatttagtgc tcgagaccgg ctatcagctc gctcagaccc gggaacgggt
     4561 gcgcgaactg gagggactgc tcatgggaga agaactcctg gtggcgcgca ttaaagatct
     4621 ggaagtcgaa gcccagaaag ttttggaact ggaaacctgg ctcgatgctt tgcgtgccga
     4681 aaatgagcac ctcaaaagac cctggtggaa gaaactcctg ggtttgaatt agagacgctt
     4741 ctttctatct ctatatgccg ctcgctcagt cgggcggcat ttttgtttga cacagggctg
     4801 ccacagtttc gaccggacat taacacagcc tgtccctagt atgggacaaa cccccaagga
     4861 gagttgtgaa atggcaaatt tcgatgaacc gcagaaggtg aaaccggagc tggagacaga
     4921 acgcagtccc ttaaatttgg ctgatctgtc tatcaatatg ccgggatcta tttctcatgg
     4981 acgggacctg gtctccggtt ctgacgccgg gaaggctctg ccgggactga tgctggttga
     5041 aaacgcgggg gctgagaccc atggcgacgg tcgcagagtt ccgaatgcag atggttcaga
     5101 gaaattcaat tttttgccga cggaagatcc cgatagtaaa tggtatctgg acggtctggc
     5161 taaaccaggc aaataccttg gtgattcaga aactctccat gataaggagt tcgtcgccgg
     5221 aggcggtaag gctccggacg cgggagcccc cgctcatgag ccgggtggct ctgattatca
     5281 ggataatggg gcagtgcgcc cgggaaaagt tgattcagcc gattcggata gcccctggta
     5341 cagcgacggc acggccagtg gcaaataccg ttttggtgac aggaacaccg ctctagatta
     5401 ggtcagctgc cggcgctgca tgccgaaggc ttatctacat gtacgtccag accggctttg
     5461 cgccaggcca ttgtgccgcc ctctatgttt tccagtttct cgaaaccatg ttgtagaaga
     5521 agactgacgg ccatggtgga gcgataaccg ccggcgcaga ttacgtgcag ggtggtatcc
     5581 ctgtccagat cgctaacggc tgccgccagt tcgctcaggg ggatggagcg cgcacctggt
     5641 acatgcccgg ctacgtattc ggctttgcgc ctcacatcca gaacctttat ggacgggtcc
     5701 cgggcaattc tttctgccag ttcctcgacg tgcacttgcg gtactgaaac cacggtgaag
     5761 cctgtgcgga agtcttccag atagccggaa acgttttcca gaccggccct ggccaggcgc
     5821 accactgctt cctgcgcttg ctcttttgtg ccgatgatga tcttttcgcg atcggcgtcc
     5881 accagtatgc cgctgaagct ggcgaactga ccgcccagac cgatattgag cgcaccggcc
     5941 ggatgtccct ggcaaaactc agccgggctg cgcacatcca gaagcagggc accttctttc
     6001 tgtttggcca gcaccgtctc cgacgtcatg gtctgaagcg gcggcagttc ggccagaggt
     6061 ctggctccct ggcgattggt ctgaaccgcc agcccgaaat atttgggaat ctccggtaac
     6121 tctctggtca aagattcgat gaaactgtct ttgtccatgg acttgagggc ccagttgaat
     6181 ttgcgctgct ctcccagggt ggatgagcgt tcctgcgaga tatttttgcc gcagagggag
     6241 ccggcgccgt gagccggata gacttccacc gcatcatcca gaggcataat cttcttgtgc
     6301 aggctgtcgt agagcagacc tgccatttct tcaggctcgt aaccgtactg gcagacaagg
     6361 tcggggcggc ccacatcgcc tacaaaaaga gtgtcgccgg tgaaaagcat cttcttagag
     6421 ctcatggcgt cttcagccag cacgcagatg ctttccggcg tatgtcccgg cgtttccaaa
     6481 attgtgaggg tcaggtcgcc gaggctcagt ttctgtccat cccgggcggc aatatgcggt
     6541 agttccaccc tggccttgtg ggagaaaact atttccgctc cggttttctg ggccagctca
     6601 ttgtgcccgg aaacgaagtc ggcgtgaagg tgggtttcaa taatgtactt gagcttgagt
     6661 ccctgagact gaagttccgt caggtattcc tccacatccc tctgggggtc gatgacgcaa
     6721 gcttcctgtc cggagcccag gacataggag gcatgggcca ggcaaccaag gtaaaactgt
     6781 ttgaggaaca ccgagacctc ccgccccgag gggctcttta tctataggga agtagattat
     6841 atcgctttgg ctgagggcca gaagcgttgt gcctgatccc ggtggaaaac cctgtaaaat
     6901 ttatctctac gaaattgaga attgcactgg agactcgatg gcttctaaga atggattttt
     6961 gcccccaccg ctgccgagcg ggttctttac ttcctgctgt ctggcccttt tgacaagttg
     7021 gagctcggcc tgcctggcat tgggcggcga taaccatgaa gaagccagtc ggtccgagat
     7081 tgaaagtgga gtcaggctgc ctgtcaatgt tttgcctgaa cgctacgatc tctactttga
     7141 gccggacttt gaaaagggcg actttgaagg cagtgagacg atactggtgg acgtgaaggc
     7201 acccacaagc acgatttctc tcaatgagca ggcgctttcg gtttacgacg cttccatcag
     7261 tcccaaaggc agtcagggcg gtttcgtcaa agccgacata gtcagacagg atgataagca
     7321 attagttcta ctgaagttcc ccagtcccct atcgaaaggg aagcatgagc tttccatcaa
     7381 gttttccggt cgcctcggca gcaaactggc cggtttctac ctttccacct ttaaggacaa
     7441 gcaggggcgt gaaaagaaga tagccaccac tcaaatggag cctaccgacg ctcgctccat
     7501 gttcccctgc ttcgacgaac cggctttcaa agctcgttac cgtctttcag tttgcatacc
     7561 gaaagagatg acggcggtaa gcaatagcgc cgtggaattt gaaaaagaag ataagcgcaa
     7621 agacaagaag gttctctctt tcaagacgac gccgccctac tctacctacc tctttgctct
     7681 ggccatcggc gacttcaaag aggtaggtag agtagggaag taaatggcaa gaaaatcaga
     7741 gtgtacgccg taaacggcaa ggagcatctg actgcctacg ctcttgatgt ggcggccagg
     7801 cttttgcctt actatgaaag ctatttcggg gccgagtatc ccctggataa acttgatttg
     7861 atcgccattc cagacttttc tgccggtgcc atggagaatc ccggtgccat cactttccgt
     7921 gagacggcat tgctctgcga cgagaaggcc tccacggcca tgcgcatgaa tgtggcaggt
     7981 atcattgctc acgaaatggc gcatctttgg ttcggagatc tggtcaccat gaagtggtgg
     8041 aacggactct ggttgaatga ggcctttgcc acctggatgg ccaccaaggc cgtgaacgtg
     8101 cttgagccgc agtggcgagc ctatgatgag tttgctctgg ccagggtggg tactctcgat
     8161 gcagacggtc tgattgccac aagaccggtg gaatacccgg taaaatcacc ggaagacgcc
     8221 atggaaatgt ttgatgaaat cacctacgaa aagggtggtt ccctgctcaa gatgcttgag
     8281 acatatctag gcgagaatac ctttcaggaa ggcatcaagc tctatatgaa agagcatgct
     8341 tttgctaatg ccaccaccgg cgatctatgg cgagctcttt ccgccagtgc cgctaaggcc
     8401 ggcaagaagg tggatgtggg agcgatgatg aatagttggg tcttcactgc cggttgcccg
     8461 ctcctggact tcagccttga accggtccgc tccggcaaga aagagatggg taaaacctat
     8521 ggcgttaaat tgtctcaacg gcgctatctg cgcgagaagg tgagtctcaa gggacaagag
     8581 agtttgtgga ctgttccgct tatgttgaaa ggcgacggtt tcagtcagcc gctcttgttc
     8641 tctaaaaaaa cggagtcaat taaagtcaag gccgaaagcg cgccgctttt tattaatggt
     8701 accggttgcg gcttctttcg cactgccttt gatgagacca gttttgccgc ccttgtgcaa
     8761 agcagccgtg cactgcctga acttctcaac tgtcaggaac gcatcagcct ctttagcgac
     8821 ctctttgccc tggctgccag tgatggttcc tccgaccccc aatcgaaagc catgaaccgt
     8881 tatttaacgg ccctcgccaa attgaaagat gaggaagacc cctatgttct atctcttttg
     8941 atcggacaag tgcaattttt cgagagcttc ctaccggctg acgagaagct ggagaaggct
     9001 tatgcctcct tcgtctgtgg gcagttaaaa gggcaccttg agcgtctggg ctttgagagg
     9061 cgcaaaaacg acacggaatt gcagtgccaa ttgagaggac aattagtgcg cgtgcttggc
     9121 actattggtg gtgaccagca gtgcatcgac ttttgccgcg gtctccggga cacttacctc
     9181 accgacgaaa aaagtcttga ttcagacttg ctttcagcca ttgtcaccgt agttgcctat
     9241 aacggtgacg gcgaagatta tgccagaatc ctgcaacttg ccaaaatggg cggcacgccg
     9301 gagtcctacc agcgtaatct gggtgcccta accgcctttc gcggcaagga acagtttgaa
     9361 gccagtctgg ctatggtgct cggcaaagac gtgcgggctc aggatgtgac caggcagtta
     9421 agccggctct tgcataatcg tgccgcgcaa aaggctacct ggaatttcct ggaaagcaac
     9481 tgggagaaac tcaagggtaa actcgatccc aagcatttgc cggatctggt ggaggctgct
     9541 tcagtattta atcaggaaga agattacaag cagcttgaag cattcataaa ccagcacccg
     9601 ctcaagaatg ggcggcgctc ggccgctaag acccttgagc ttgtccgtat tcgtatccag
     9661 tttagaaaga acgctttgcc gactttcatt aaggctttgc cctgaggaaa aatgaccgtc
     9721 ctgggaagat tcaagcagag agggaagtgg ggcggcatcg gcttgctgct cgcttttgtg
     9781 ctgaactttt gcggcggctc ctgctcgggg gccattgcca agcccgtgct tgttttctgg
     9841 tcctggtatc acgatgacga cctctcttca gcccggggag aagtggctta cctgaccaat
     9901 cgcattgtgg tgaacggctc cgagatgaaa gattatcctc gcaccaaccg cctaaaagtc
     9961 agacctggta ccagaagaac ggcggtggta agggtggagg tgcggaagct tgagggcgcc
    10021 aagagccggc ttctggcccg gcgcatcgtc agtggtgcct tgcgcagtca ggtcgaagcc
    10081 ttgcagattg attttgatgc gcgcatgaat gagcgagcct tttatctgga gttgctctcg
    10141 gacatcaggc aacaattgcc acagggcatc aagctcagta tgaccgccct ggcctcctgg
    10201 tgcatgcaag ataattggct gcccggcagc aattccttag tggacgaagt ggtgcccatg
    10261 tttttcagca tgggtagccg tcaacgtcag gctgaggatt tcctgcaaga gaaattgccc
    10321 ctggtgcccc tgggaccgag atgtatcgga ctgagtatgg atgagcctgc caccatcgaa
    10381 aagctgtccg tgcgtttgcg tcagtttgac cgaatttata tcttcagtag aagaccctgg
    10441 acggggacta ctcttgaaac tagcctggga atgttaaagc gttggatata ggagattacc
    10501 gtgtttaagc ctgctaactc aaaaagaccc ctgctcagga tggcaatcgg gctctgcctc
    10561 agcttgaatc ttctgccggt gttggcctgt ggtccctttt tcgatgatgc cgtcttttac
    10621 ctccggtctc accccgatgc tcccatgaag cgctttctct cgggctctct gggggtgctg
    10681 gaacccacat atgcacgtag ttaccttatc tttgcctatc gttatcttca ggacaagcct
    10741 ctgacggcgg cagaaagggc cagtgcctat gatctgcttt gctatcgcct tggcatggct
    10801 aatatcgatc ctcgctccga ggtctctccc agggaggctg gcagtggcgt cgatgtctgg
    10861 aacaatgcca ggaaaaaagt taagggtgcc cgtgtacgag agttttccag ttgggacact
    10921 tttcgcaaca tcgatggcga agactacatt tcctacttga actgcactcc ggatgccttt
    10981 atcacggcag ccgccactct cgataagcgc atcagcacct atggtgccga cagtgccgag
    11041 gtgaaagcct ggcttgaggc tcaagatcaa gtattcagtc actgtagctc aagcggctat
    11101 aactatgaaa agaaacagca gaatccgccg ccgccgtttc ccgccacctt gcctgccacc
    11161 gtaccgctgg cacagaggca agatcgtttc tatcaactgg ctgcctcccg cttctatgcc
    11221 ggagagttcg aagtggctcg cgctctcttc cgcgaaatag ctgccgacaa agcctcgccc
    11281 tggcaccaga ttgccgaata catggtagcc cgcacttaca ttcgcgaagg tacgctggct
    11341 aaagatgtgg ctaagggtca gaagctgttg cagcaggcta tggatattct cgatggtctt
    11401 atgcgcaagc ctgaatttgc ttccttttcc agctctatag aggatctgcg ccatttcctc
    11461 ttgattcgcc tgaggcggga cggtacattc aaagatctct cgagcaagat tgtcagtgac
    11521 gggctgggag acaagttcgg caagtctgtg ggtgactaca ccttcattct ggaccgctat
    11581 ttcaatgaaa gcggcgatga gtcatcggag acccatgaaa ttgagg
//
LOCUS       DAOQNZ010000002         8757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_2975_length_8757_cov_3.754424, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000002 DAOQNZ010000000
VERSION     DAOQNZ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2975_length_8757_cov_3.754424"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1175
                     /locus_tag="PKW73_00070"
     CDS             168..1175
                     /locus_tag="PKW73_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA71701.1"
                     /translation="MKETLSKNCYPVVIAGAGPAGVSVALGLLEARIEVLLIDAAPDM
                     GGQLPSIPSPITNFAGGYKVTGRTAGRDMKEALLAAAERAGEDGVSLTYLPNYQIAKF
                     DARTNELQLKSTVQAKDLTVCGERVVFCTGYRERRLQLPGQAECEQAIYYHGKGEDGG
                     TAVVIGGGDSALLKLLAVAPQMKNVILIHRNKHFKARPDVIAKVSNLPNLEVFLGSQV
                     VALARNSQTGKIAVTIETMDGGGRQTRPADWLAVKVGYLPNTEFLTDSLLLEKGHVVV
                     DSALHAYDKEEHLLPTVFAGGDIVNGSFPRIATACGHGMSIAAEIIKATFENGSLPDG
                     K"
     gene            complement(1155..2504)
                     /locus_tag="PKW73_00075"
     CDS             complement(1155..2504)
                     /locus_tag="PKW73_00075"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNA71702.1"
                     /translation="MKNRTAGADDKEASFAVGDKIEAVKESELLTADGSSAASSEIAK
                     VDEAAPDKHKSVYHRKGFGLKDEVEGELKKEYESDLVAELRASGNVISRNGMTIKLAE
                     AFGFCWGVDRAVSMAYETRKHFPDRRIWITNEIIHNPVVNANLREMGIEFLGVRSDGS
                     KDFSSIGRGDVVILPAFGASVEEMRIIEGRNCEIVDTTCPWVSRVWNRVVKYAAGFDH
                     GYTAIIHGKPNHEETVATASRAHCYLIVRNIEEAGLVASYILSGIDGAGGDREAFMKR
                     FQDAVSPGFDPDVHLQSIGIANQTTMLKGETEQIGKLFEKTMLQKYGPQSIDKHFISP
                     GDTICDATQERQDAMLKLVDEKLDLMLVIGGFNSSNTGHLQEIAEKSNLPSYHIDGAD
                     CIGPGNQIRFKPLDGDDCQSKVNFLPDGEVTIGLTAGASTPDQLVAQVLEKLFSIRQ"
     gene            2725..3141
                     /locus_tag="PKW73_00080"
     CDS             2725..3141
                     /locus_tag="PKW73_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71703.1"
                     /translation="MKNRNNLKALTCAVLAVAALASTVNVQALADEPKDMASNAVLFP
                     VRAASIASGLVIGIPVAIARRSSNRVIEYTGNFADKMGGKDHIPPKVFGSIFGLPFGL
                     LVGTGEGLYYGGKNAFSNSVEKPFSLAAFSLDKELE"
     gene            3373..7095
                     /locus_tag="PKW73_00085"
     CDS             3373..7095
                     /locus_tag="PKW73_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71704.1"
                     /translation="MSIDKDRVLRLNCPGYGEESYLNLGQAKSLEHYQVIIANPVSLL
                     HLFDKGPEAARRINHFLQEGINQLNVPDDALIQELINESDARLEELIPFLTQGGLLIY
                     FLCRPFILAGPTISVDNYDWLSVYAPSAKVPEGKGPRQMSAVSHGRIVEPTEEGELSE
                     MADYLHLPGIEWNTIIRTDFLSSNYSVLATAGPKKCIAAQFWAGDKGGKVIFLPAPYS
                     PDFDKVLMEGVNKWYQNTKPGSSAQSSPPPLESLDLSQLVASAEDSLKILDQIETPKS
                     DINIGTAPLTEPTPPAQPTADKKPEVKQAPKGALKSLFANKGLLDDDDDDELEPSAPA
                     AASPEVAKVTAEDILKSVSMEAERLSKEFESSPPAVPQSQAAHAQVGREESIALAAPS
                     EPIQESQDRPPFTLAAIATTSPPTPVSDEVKPPSDQADLAIDDNLDLDKIIMASMELS
                     KKIDDQTTSVQDQPEGAAPAAAPTINPADVIDIDSSQSSTAIPAENSNAAVAGFELKR
                     ATADMDLSEFAETARQLVQQANEIESATREPGKPPVQTPMTKINEQLKHSESSATLDV
                     SEAIDVTSEPVVSPSMLEQLQMVHPAADVKAESTPEEAQNQLLNGMIKDMLIHQEEEE
                     INPKSSPSAPIEGDITPQRPDKPATSMSMDEFRQTFEFLKEEEVAAAASQAAAEAAEF
                     QAAAKPSALQQNTGADQSEAPDIFAAPPESEVRTSEGRGSLRSIVQSLGQTGLPESPA
                     SAPVGPAWLSGTESGSPANDQSAFDATSTQESIPALDVAQEIIQPTYQAELLGQPDLL
                     TPEPVRESPSQPEAPAVNTPPATFAPALPAVAPAPPTTFAPALPVAAMVPPSPPPQPE
                     LPPLPASLMAEEPAPAVPAPPVVIPQATVPVTEPIKEMQSQPAPTLQALQAPPAPPLN
                     QAPPMMQQPAPPAAVPAPPLEPPKPAPAPAWAAAPDKAPAFTPPAAPQPEAKAPPAFV
                     APPPAPSPTVSPGNGNGSNSNQALPPLPELQEDPDTPSPAKELMIKMEELTQTMNLTW
                     TQDFSFPYVDALKGEHGQMMEQLRQMQLRISALDSRIRAIEGLKHVLLTGENDPLLAA
                     SQEVLSRLGWTVSPSRSNPGELWLSRGDQIDAIARVVRSPGAANRSEIAQLAESVIAF
                     WDEYEIEPKGILLAQTWFSKTPAERTDPDFSPALQEFAGKKNLCLMSSLQLLGMYKDL
                     EMGGMPVEEMRKRMLETNGRLMGFTLDNNLAPGQTV"
     gene            7136..8353
                     /locus_tag="PKW73_00090"
     CDS             7136..8353
                     /locus_tag="PKW73_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71705.1"
                     /translation="MSIPTLLVCDGGESRQDVLEKMIKAEPGLKYLATVGLVNGTRAV
                     EQAIKVGGKLIWIDLEDEPDKAIDLIAEVRNMFPQANLLASANGVAPDIIRAALNLGA
                     LDVVRPETAAQQLQNLVARINGTAPAAAPAPVPAPAPVPAPAPAPVPAPAPAPASAPA
                     AGGSKWGDLDSIPTPKAAAKAQEATGEINIGQMPRQDIDVSAAREMIQAQAQQQQVAA
                     PAPMPGPMPAPAPEGGSRWGDLDSIPTPKANGAAPAPAPAPAPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPAPAPAPAPAPAPAPAPAPASEPAAAAPGEGASKWGDLDAIPTPKAVT
                     TEMEKVTRPPADPSAPPPPLPTPKPRSDVNLRPSPNKAEMYNMPSTPAWMVAVILIVI
                     MACVGYAFLSRPH"
     gene            complement(8360..8698)
                     /locus_tag="PKW73_00095"
     CDS             complement(8360..8698)
                     /locus_tag="PKW73_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNA71706.1"
                     /translation="MSDQTEPVIDCSRLKRLDQQGKLELLEPLHGWSINADGKLCRTF
                     VLQNFAESLALANLVGAVAEEIGHHPDLLVRWGSLSVEIFTHSLSHENNAALTEADFF
                     LAARINELRA"
BASE COUNT         2197 a         2584 c         2189 g         1787 t
ORIGIN      
        1 atgcctgccc ggccctttag actttctcgc cccaccgctt aaactactaa accaaggtct
       61 cgacaggcag gcaaactcta acaaaattca gcgcatcatg ccagtctaat tcacacccca
      121 agcgaacctg aaaccgcagg taaaccggta tcaaaaggga gcccaccatg aaggaaactc
      181 taagtaaaaa ttgctatcca gtggtcattg ccggtgccgg accggccggg gtgagcgtgg
      241 ccttaggtct cttggaagcg cgtatcgaag tattactgat tgatgcggca ccggatatgg
      301 gcggtcagct tcccagcatt ccctcaccca taaccaactt tgccggtggc tataaggtaa
      361 cgggaagaac agccggtaga gatatgaagg aagcccttct ggccgccgcg gaacgagccg
      421 gagaggacgg ggtgagcctt acctatttgc ccaattacca gatagcaaaa ttcgacgccc
      481 ggacaaacga actgcaactg aaatccaccg tgcaagcaaa agacttgacc gtgtgcggag
      541 agagagtggt cttttgtacc ggctatagag agcggcggct gcaactacca ggacaagcgg
      601 aatgtgagca agcaatctat tatcacggca agggcgaaga cggtggcacc gccgtggtga
      661 ttggcggcgg cgacagcgca ctgctgaaac tactggcggt ggcgcctcaa atgaagaatg
      721 taatcctcat ccatcgcaac aagcacttca aggccagacc ggatgtgatc gcaaaagttt
      781 ccaatttgcc caatttggag gttttcctgg gaagtcaggt tgtggctctg gccaggaaca
      841 gccaaaccgg caaaattgcg gtcaccatcg agaccatgga cggcggtggg aggcagactc
      901 gaccggccga ctggctggcc gtaaaagtag gctacctgcc gaacacggaa ttcttaaccg
      961 acagcctcct attagagaaa ggacatgtcg tagtggactc agccttgcat gcctatgaca
     1021 aagaggaaca cctgctaccc accgttttcg ccggtggcga catagttaac ggcagctttc
     1081 cccgtatcgc tacagcttgc gggcatggca tgagtattgc cgccgaaatt atcaaagcta
     1141 ctttcgaaaa cgggtcactg cctgatggaa aatagtttct cgagtacttg cgccaccaac
     1201 tggtccgggg tggaagcgcc ggctgtaaga ccaatcgtca cctcaccatc cggcaagaaa
     1261 ttgactttac tctgacaatc gtcgccatcc aagggtttga aacgaatttg attacccgga
     1321 cctatgcaat cggctccgtc gatatggtag gaaggcaaat tacttttctc ggctatctct
     1381 tgcaaatgac cggtgttgga agaattgaag ccgccaatta caagcatcag atccagcttc
     1441 tcatccacca gtttgagcat ggcatcctgc ctctcctgag tggcatcgca gatggtatca
     1501 ccaggactga tgaaatgttt atcgatggat tggggaccgt atttctgcaa catggtcttc
     1561 tcaaaaagct tgcctatttg ttcggtttcg cctttcagca tagtggtttg attagctatg
     1621 ccgatagact gcaaatgcac atcagggtca aagccaggtg atactgcgtc ctggaagcgt
     1681 ttcataaagg cttcccgatc gccgccggcc ccatcaattc ccgaaagaat ataagaagct
     1741 accagaccgg cttcctctat gttgcgaact atcaagtaac aatgagcgcg ggaagcggtg
     1801 gccacggttt cttcatggtt cggtttgcca tgaataatgg cggtataacc atggtcgaag
     1861 ccggcggcat atttgacgac tcgattccat acccttgaca cccatgggca ggtggtatcg
     1921 acaatttcac aattgcgacc ttctattatt ctcatttctt ctactgaagc gccaaaggca
     1981 ggcaagatca ccacgtcacc ccggccgatg gaagaaaagt ccttgctgcc atcactccgc
     2041 actcccaaaa actcaatgcc catttcccgc aaattagcgt ttaccacagg attgtgaatg
     2101 atctcgttgg tgatccagat gcgccggtcg gggaagtgtt tgcgcgtttc ataggccatg
     2161 gaaacagccc gatccacccc ccagcaaaat ccaaaagctt cagccaactt aatggtcatt
     2221 ccattgcggg atattacgtt tccggaggct cgcagctcgg caactaaatc cgattcgtac
     2281 tctttcttga gttcgccttc tacctcatcc ttaaggccga agccctttcg gtggtaaacc
     2341 gacttgtgct tatccggcgc cgcctcgtct accttagcta tttctgaact ggcggctgaa
     2401 gagccgtccg cggtaagcag ttcagactct ttgacagcct caatcttgtc gcctacggcg
     2461 aaggaagcct ctttatcgtc ggctccggca gtcctgtttt tcatggaaga cattgtaaag
     2521 gcgatccccc gggcatccaa tgcatataga atcaaatact tggcagcttt aaaagtacaa
     2581 tcttagcaag acccgggtac ggatgtggta ccaacgggct gaaccagtgc tatcatgaga
     2641 cgcactaaca ttactagtat ctcgccaagc tctgccagat tcaatcaaat caactaattc
     2701 acctgaatgg atggaggact cccagtgaaa aatcgcaaca atttgaaagc tttgacctgt
     2761 gccgtattgg ccgttgccgc cctggcatcg accgtaaacg tacaagccct tgctgatgaa
     2821 cccaaagaca tggccagcaa cgccgtgctc ttcccggtac gcgccgccag catcgccagc
     2881 ggtctggtga ttggtatccc ggttgcgatt gcccgcagaa gttccaaccg cgtaatcgaa
     2941 tacaccggca acttcgccga taaaatgggt ggcaaagacc acattccccc gaaagtattc
     3001 ggttccatct tcggtctccc cttcggtctc ctggtcggaa ccggtgaagg tctctactac
     3061 ggcggtaaaa acgcattctc caacagcgta gagaagccct tctccctggc tgccttcagc
     3121 ctcgacaaag agctcgaata agcattcctc tcttttgagg gtcagtggct tataaaaaaa
     3181 cccgggcaac cgggtttttt cttttggcag gcggaaagcg gttccttgac ttgacatcaa
     3241 actcactgct ttttagcgtt tgccttttga atagtgggta taagacatgg ggagttaccc
     3301 tctacttttc agcttggcaa tccgacctgt gccatctgag tgagagcggg gtaccgtgaa
     3361 agggaaaaga ggatgtctat agataaagac cgggtgctga gattaaattg cccgggctat
     3421 ggcgaagaat cgtacctcaa ccttgggcaa gccaagtcgc ttgagcatta tcaagtgata
     3481 attgcaaatc cggtcagcct tttgcatctc tttgacaaag ggccggaggc ggcaaggcgt
     3541 ataaatcact tcctacaaga aggcatcaat caactgaacg tgccggacga cgcattgatt
     3601 caggaattga tcaacgagtc ggatgcccgc ctggaggagc tgataccctt cctcacccag
     3661 ggcggtctcc taatttactt cttgtgccga cctttcattt tggccggacc gactatttca
     3721 gtggacaatt acgactggct ctcggtttat gccccttccg ccaaagtacc ggagggcaaa
     3781 ggccccagac agatgagcgc cgtcagccat ggtcgcatag tggagcccac ggaagaaggc
     3841 gaactctccg agatggccga ttacttgcat ctccccggca ttgaatggaa cacaattatc
     3901 agaaccgact tcctttccag caactattcg gtgctggcaa cagccggacc aaaaaaatgt
     3961 atcgccgcac agttctgggc cggggacaaa ggcggtaaag ttatcttcct gcctgcgccc
     4021 tattcacctg acttcgacaa agtcctcatg gaaggagtca ataagtggta tcaaaacacc
     4081 aagcccggct ccagtgctca aagcagcccg cccccattag aatctctcga cctttcccaa
     4141 ctggttgcca gcgccgaaga ctcactcaag atcctggatc aaattgaaac acctaaatca
     4201 gacatcaaca taggaacggc tcccttgact gaaccaaccc ccccggcaca gccgaccgca
     4261 gacaaaaaac ccgaggtcaa gcaagcgccc aaaggtgccc tcaaatcact ttttgccaat
     4321 aagggactcc ttgatgatga cgatgacgac gaattggaac cgtctgcgcc ggctgcagcc
     4381 agccccgaag tagcgaaagt tacggctgaa gatatactca agtcggtatc aatggaggcc
     4441 gagcgcctca gtaaagaatt cgaatcatca ccgccggcag tgccgcaatc gcaagcagcg
     4501 catgcgcagg tcggtaggga agagtccatc gctcttgcgg ctccttcaga accgattcaa
     4561 gaatctcagg atagaccgcc cttcacgctg gcggccatcg ccacaaccag cccaccgaca
     4621 ccggtctccg atgaagtcaa gccgccctcc gaccaggctg atctagcaat tgatgacaat
     4681 ctcgatctcg acaaaatcat catggcctcc atggaactat cgaagaagat cgatgaccag
     4741 acgacctcgg tgcaggacca accggaaggt gcggctcccg cagcagcacc gaccatcaat
     4801 ccggcggatg tgatcgatat agattcatcc cagtcttcca ccgccatacc tgcggaaaat
     4861 tcgaacgcag cggtggccgg cttcgagctg aagcgagcta ccgccgatat ggacctgtcg
     4921 gaatttgctg aaactgccag gcagttggta cagcaagcca atgaaattga gtcggcaacc
     4981 agagaaccag gcaaaccgcc ggttcagacc cccatgacca aaataaacga gcaactcaag
     5041 catagcgaaa gcagcgcgac ccttgatgtt tcagaggcca tcgatgtaac ttccgagcct
     5101 gtagtaagtc cgtccatgct cgaacaacta caaatggtgc atcccgcagc cgatgtaaaa
     5161 gctgagtcga caccggaaga ggctcaaaat cagttgctta acggcatgat aaaagacatg
     5221 ctcatccacc aggaagaaga agaaatcaac cctaagagtt ctccatccgc accgatcgaa
     5281 ggcgatatca cgccacagcg tccggataaa ccggccactt ccatgagcat ggatgagttt
     5341 cgtcagactt ttgaattcct caaggaagag gaagtggcag ctgctgcctc gcaagcggcg
     5401 gcggaagcgg ctgaatttca agctgcggcg aagccgtctg ctctacaaca gaatacaggt
     5461 gcagaccaga gcgaagcacc ggatattttt gcagctcctc ccgaatcgga agttcgaact
     5521 agcgagggtc gaggcagtct cagatctata gtgcagtccc tgggtcagac cggtctaccg
     5581 gaaagccccg cctcggcacc ggtcggtccg gcctggctat caggcactga atccggctct
     5641 cccgctaatg accagagcgc tttcgacgcc acttccaccc aggaatcgat tcctgcactg
     5701 gatgtggccc aggaaataat tcaacctact tatcaggccg aactcctcgg tcagcctgac
     5761 cttttgacgc cggaaccggt cagggaaagc ccctcgcagc ctgaagcgcc ggcagtcaac
     5821 acaccaccgg cgacatttgc accagccctg ccagcagtgg ctcccgcacc gccgacaaca
     5881 tttgcaccag ccctgcctgt tgccgcaatg gttcctccca gtccaccacc ccagccggag
     5941 ctaccgccct taccggcctc tctgatggcc gaggagcccg ccccggcggt tcccgcaccg
     6001 ccagtggtga taccgcaggc gacagtcccc gtgaccgaac ctataaaaga aatgcaaagt
     6061 cagccggcgc ccactctaca ggcgctgcag gcgccaccag caccgccact caatcaggca
     6121 ccaccgatga tgcaacagcc cgcaccaccg gccgccgtac cggcaccacc gctcgaacca
     6181 cccaagccgg cgcccgctcc ggcctgggcg gctgcaccgg acaaagctcc cgctttcact
     6241 cctccggcgg caccacaacc ggaagcgaaa gcaccaccgg cttttgtggc tcctccgcca
     6301 gctccctcac ccaccgtctc gcccggcaac ggtaacggca gcaattcaaa tcaggctttg
     6361 cccccactgc ctgaattgca agaagatcca gacactccct cgcccgctaa ggaactaatg
     6421 ataaaaatgg aagaactcac gcaaacaatg aatctcacct ggactcagga cttctccttc
     6481 ccttatgtcg atgctctcaa gggcgagcat ggacagatga tggaacagct gagacagatg
     6541 cagttgcgta tttccgccct cgactccaga atcagagcca ttgaaggcct caaacacgtg
     6601 cttctcaccg gcgaaaacga tccacttttg gcggcttccc aggaagtact atcgcgactt
     6661 ggttggaccg tctctccgtc tcgctccaac cccggcgaat tgtggctttc tcgcggcgac
     6721 cagatcgacg ccattgccag agtggtgcgc agccccggag cggccaaccg ttcagagata
     6781 gcccaactgg ctgaatcagt cattgccttc tgggacgagt atgaaattga accgaaaggt
     6841 attctcctgg ctcaaacctg gttcagcaaa actccggcgg agagaaccga tcctgatttt
     6901 tccccggctc ttcaggaatt tgccggcaag aaaaaccttt gcttgatgtc ctcattacag
     6961 cttctgggta tgtacaaaga cctggagatg ggaggcatgc cggtagaaga gatgcggaaa
     7021 cgtatgttgg aaactaatgg cagacttatg ggcttcactc tcgataacaa cttggcaccg
     7081 ggacagacgg tgtaaactat caacgtggca ctggcaatga aggggcttgt caaccgtgag
     7141 cataccgacc ttattggttt gtgatggcgg cgaatccaga caggatgttc tggagaaaat
     7201 gatcaaggcg gaaccagggc ttaagtacct ggctaccgtt ggtcttgtaa acggcacaag
     7261 agccgtagag caggccataa aagtaggtgg caaactaatc tggatagatc tcgaggacga
     7321 gccggacaaa gccatcgatt tgatcgccga agtaagaaat atgtttcccc aggcaaatct
     7381 gctggcctcg gcaaacgggg ttgctcctga cattatcaga gcagccctca acctcggcgc
     7441 tctggatgtg gtgcgaccgg agacggccgc tcagcaattg cagaatctgg tagccagaat
     7501 caacggaacg gctccagctg ccgctccagc tccagtaccg gctccagctc cagtaccagc
     7561 tccagctccg gctccagtac cagctcctgc tcctgctcct gcttcggctc cggcggccgg
     7621 cggctcaaaa tggggcgacc tggactccat cccaaccccc aaagcggcgg ccaaagcgca
     7681 ggaagcgact ggcgagatca atatcggaca gatgcccaga caggacatcg atgtatccgc
     7741 agccagggaa atgattcaag cacaggccca gcagcaacag gttgccgctc cggcaccgat
     7801 gcctggtccc atgccggccc cggcgcctga aggcggttcc cgctggggcg atctggactc
     7861 catcccaaca ccaaaagcca atggagcagc accggctcct gcacctgcac cagctcctgc
     7921 tccggctcca gctcctgctc cagctccagc tccagctcca gcaccagctc ctgctccggc
     7981 accggctcct gctcctgcac cagcaccagc tcccgcacca gctcctgctc cggcaccggc
     8041 tcctgctcct gcttcagaac cagctgctgc tgcacctggc gagggagcct ctaagtgggg
     8101 agacctggat gccattccta ctcccaaggc cgtcactacg gaaatggaaa aggtgaccag
     8161 accaccagcc gatccgtctg caccgccacc gcctttgccc actcccaaac cacgtagcga
     8221 cgtaaatctc aggccctctc ccaacaaagc cgagatgtac aacatgcctt caacgccggc
     8281 ctggatggtg gcagtgatcc tgattgtaat tatggcttgt gtcggttacg ccttcttatc
     8341 gcgcccccac tgataatatt caggctctca attcgttaat gcgagcggct agaaagaagt
     8401 ccgcttcagt aagcgcggca ttattctcat gggagagtga atgggtaaat atttcgacag
     8461 acagagaccc ccagcgcacc agcagatccg gatggtgacc aatttcctcc gctaccgcac
     8521 ctaccaaatt ggccagggcc agggactcgg caaaattctg caagacaaaa gtacggcaga
     8581 gcttaccatc agcatttatg gaccaaccgt gcagcggctc cagtaactcc aatttcccct
     8641 gttgatccaa cctctttaaa cgagagcagt caatgacagg ttcggtttga tcggacatgg
     8701 gcatactcct gagccggctc aagacataag ggaaagccaa ccctgattat cagccgt
//
LOCUS       DAOQNZ010000003         8703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_2995_length_8703_cov_3.533649, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000003 DAOQNZ010000000
VERSION     DAOQNZ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2995_length_8703_cov_3.533649"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKW73_00100"
     CDS             <1..384
                     /locus_tag="PKW73_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71707.1"
                     /translation="VQEVRCTYDPETLGANPVGRKVKGTIHWVSARHAQDAEVRLYDR
                     LLSLEAPPAGKDSEFLNAINPEALQTLTGCKLERSLAEAKPGERFQFERQGYFVADVK
                     DSKPGMPVFNRIVTLKDSWARSQKK"
     gene            complement(467..538)
                     /locus_tag="PKW73_00105"
     tRNA            complement(467..538)
                     /locus_tag="PKW73_00105"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(504..506),aa:Gln,seq:ctg)
     gene            1194..2321
                     /locus_tag="PKW73_00110"
     CDS             1194..2321
                     /locus_tag="PKW73_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNA71708.1"
                     /translation="MTQTTTPNTAAASTTSFGLALFKEEARQNKGKNVLISPASVAIA
                     LAMTMNGARQTTLSAMRSTLGFGEGETIDTINASMEALLSALADPKSGVELTVANAIW
                     AEQSMPFKKAFLDGVVAAYKARVVNADFADPATVDAINNWANENTRGKIPTILDEIAA
                     DMVMYLLNAIYFKGSWTTKFDKDMTFGGQFASEADGEVSVMYMNREADMRYSRGKTFQ
                     AVALPFGESKRVSLYVLLPNEGVDINDFVEGLSASVLTNLQESWESEVDLRLPRFKAE
                     YDVDLKDSLCALGMGEAFESGADFTGMADAAMVISTVKHKTFAKFDEDGGEAAAVTAV
                     GIALECVRMTPQVHVTRPFVAALVDEVTGALLFIGKVAKPD"
     gene            2635..4107
                     /locus_tag="PKW73_00115"
     CDS             2635..4107
                     /locus_tag="PKW73_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNA71709.1"
                     /translation="MKFDLTLDREARVELSQQIYEQIKTAILSGRLKPGDRLSSSREL
                     SEELSVSRPTVAVAFEQLSLEGYIEMRHGSGTYVQEGLSAAAAVKRKRGTVGKATRGR
                     SNSDFRREAEQMSPSTRGMESPALSFQYGIPALEEFPNAEWSRLVGRISRNASAGDLA
                     MTGEAFGLVTLRHAIADCLVRFRGLSIKPEQVIVVQGLNQGLDLVARLHLNPGAVVAM
                     EDPGYPLAPRIFRANSGRVVPVGLDDQGLHIEQLKGSSPALLNKAKLIYLTPSHQFPT
                     GITMSLPRRLELLAWAKRSGAIILEDDFDSEYQGTGKPIPALMSLDTNDQVIYSGTFN
                     QLMFPGIGVGYLIVPESLVPLYERGRRLLGEPVNLQLQQCLAEFILRGELERHWRRTR
                     VVYSERRQAFLDALERYTSGLYTVRGEQCGVFVSVTFAGRMTTEAIIKKAAQKNLGLF
                     STRDFYSRSAAPEREFIFGFGALKETAIVKGVKLLSTILA"
     gene            complement(4165..4422)
                     /locus_tag="PKW73_00120"
     CDS             complement(4165..4422)
                     /locus_tag="PKW73_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71710.1"
                     /translation="MKGRIVTLIALLSVGQALTSDLQAQAQLPQQTTVKPAKKNVFTR
                     MTGATRRVLRDNRVKSLFSKLGKKKPAATGTAPATTTAPAQ"
     gene            complement(5169..5501)
                     /locus_tag="PKW73_00125"
     CDS             complement(5169..5501)
                     /locus_tag="PKW73_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71711.1"
                     /translation="MNQTPPTCPILLEVSNLLKQGSDHQRWQEFEKAEGFYVDAITLA
                     KEQLGAEHQAVGYALNDLAQLQESMGEEIRARDTFVEALTILEKHLGNNHPVTLDIFG
                     RLHHLYRL"
     gene            6059..6937
                     /locus_tag="PKW73_00130"
     CDS             6059..6937
                     /locus_tag="PKW73_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA71712.1"
                     /translation="MSAVIEVRNLAKKFDKAIAVEDISFTIEKGEFVGLLGQNGAGKS
                     TTIQILLGLITPTSGDVRIFGYSPWKERVKVLERSNFSSAYVAMPPNLQVRENLEVFA
                     RLYGVRNRSRKIDELLSLFAVDHLARKMTGALSSGESTRVNLCKALLNDPELLLLDEP
                     TASLDPDIADKVRGLLRQIQKERGLTVVYTSHNMTDVEELCHRVLFLHRGRIIAQGTP
                     GEIVENLGESSLEEVFIAIARDTLKVAPGPSSVPGTAPDVAQASASARNDGESKSEDA
                     SVEISGRPGREKEELC"
     gene            6931..7731
                     /locus_tag="PKW73_00135"
     CDS             6931..7731
                     /locus_tag="PKW73_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA71713.1"
                     /translation="MLTVRPDRVAGLCYRYYLVLSRSSFRVMDIVFWPVMDLLVWGFV
                     SNYFSSLGGSAPQAVTFLVGGIIFFNVLYRAQQSISVSFLEDLWSRNLLSIFVSPITV
                     AEFVSATYVVGVAQALAVTLIMSVMAAFLYAFNMLSLSWYLIPLFVNLLVMGWWLGLG
                     TTALILRYGYQAEALAWAVPFLLQPVCAVFYPVSVLPAWLQSVAWFVPATHVFEGMRH
                     ILSGGNQCAVYLIKASLLNVLYWVATTIFFGAMINRARERGSLAKIVT"
     gene            8038..8385
                     /locus_tag="PKW73_00140"
     CDS             8038..8385
                     /locus_tag="PKW73_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71714.1"
                     /translation="MTCGSEHKGHDHKHGEGCGHKTVEHDGHKDYLHDGHLHNVHGDH
                     VHDHSLSVGGANAANCTPEHKCGGHDGGHKHAAGCGHEAVPHGDHVDYLVEGHLHHPC
                     GSHCDDHGPVNVK"
     gene            complement(8490..>8703)
                     /locus_tag="PKW73_00145"
     CDS             complement(8490..>8703)
                     /locus_tag="PKW73_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71715.1"
                     /translation="MQTASLRGYLLLSSHEGEQCTLGSLLKRRLAAIYKDHGVLLSRM
                     RRESEADRYYCLSRKTLSDAIMATKR"
BASE COUNT         1846 a         2174 c         2506 g         2177 t
ORIGIN      
        1 gtgcaggaag tgcgttgcac ttatgatccc gagaccctgg gtgccaatcc tgtgggtcgt
       61 aaggtgaagg gcaccataca ttgggtgagt gctcgccatg cgcaagatgc ggaagtgcgc
      121 ctctatgacc gtctgctctc cctggaagct ccgcctgccg ggaaggacag cgagttcctc
      181 aacgccatca atcctgaggc tttgcagact ttaaccggtt gcaagctgga gcgtagcctg
      241 gctgaagcca agcctggaga gcgtttccag tttgagcgcc agggttattt cgtggcagac
      301 gtgaaggaca gtaagcccgg catgccggtc ttcaatcgta ttgtcacctt gaaagactcc
      361 tgggcccgct cgcagaagaa atagcaccgg tcggagctct tcccggcgct caaaagaaat
      421 agagactggt gctaccagtc tctagtgcgt gtgacgcagt aagaatctgg ggtgcaagga
      481 ttcgaacctc ggaatacctg gaccagaacc aggcgcctta ccacttggcg acaccccaat
      541 gtagtttgat attagaagtc taggaagcat ttgttccggt gtcaatgaat tatgaatgac
      601 ctcacggagg gggttcgcat tactgactct ccggcagttt cctgtaaagg actgtaaccg
      661 atgttcgcgc cattgaaaga gtccttgact acctccgcct ttctgattct taaaattcca
      721 atcggatttg gcggtgccgg ccggcaataa aataacgccg ctccgcagag acaatccagg
      781 ccaggtttca ggggatctct gaaaataagt caaggatagc tgcattcctt gaatgattat
      841 catgacaatt gttaggttct aaaagtactt cttttcccct ttcccgagtt acccaagcaa
      901 aagcttccgt atacgccgct accgattgtt ggcttcattt ctctgctggt actggcagtg
      961 aagagtagaa caaccggttg ttggagcgct gctgattcca cgttcgaatt gagtctgccg
     1021 ttttcacttg ggtttactca ggtggcggtt caattcgggg cagggtttta cagcgagttc
     1081 cttcctggtg ggcgaggtcg tgaaaagaag tagggctgtt ctcacaggct gtgaggcatg
     1141 ccaaagcaac caatccaatt caaatgcaac cgcttagaaa gcaggaatat ccaatgacgc
     1201 agaccactac tcccaacacc gccgccgctt ccaccacgtc tttcggcctg gcgctgttca
     1261 aggaagaagc ccgtcaaaac aagggcaaga acgttcttat ctcgccggca tcggtggcta
     1321 ttgctctggc catgaccatg aacggcgccc gtcagacaac gctttctgcg atgcgttcca
     1381 ctcttggctt cggcgaaggt gagaccatcg acactatcaa tgcctccatg gaagcgctgc
     1441 tttcggctct ggcagacccc aagagcggcg tcgagctgac cgtggccaat gccatctggg
     1501 ccgagcagtc catgcccttc aagaaggcct tcctggacgg ggtcgtagcg gcctataagg
     1561 ctcgcgtcgt caatgccgac ttcgccgacc cggcaaccgt ggacgccatc aacaattggg
     1621 cgaacgagaa cacccggggc aagattccca ccatcctcga cgaaattgcc gccgacatgg
     1681 tcatgtatct gctcaatgcc atctacttca aaggtagttg gaccaccaaa ttcgacaagg
     1741 acatgacctt cggcggccag ttcgcctcgg aagccgatgg cgaagtctcg gtcatgtaca
     1801 tgaaccgcga agcggacatg cgctacagcc gcggcaagac cttccaggcg gtggctctgc
     1861 ctttcggcga gagcaagcgc gtgtcgctct acgtgctttt gcccaacgaa ggcgtagaca
     1921 tcaacgactt cgtggaaggg ctgagtgcgt cggttctgac caatctgcag gaaagctggg
     1981 agagcgaggt ggacctccgt ctgccgcgct tcaaggctga atacgacgtg gatttgaagg
     2041 attcgctctg cgccctgggc atgggtgaag ccttcgagtc gggtgccgat ttcaccggca
     2101 tggctgacgc cgccatggtg atcagcaccg tcaagcacaa gaccttcgcc aagttcgatg
     2161 aagacggcgg cgaagctgca gccgtcaccg ccgtgggtat cgctttggag tgcgtgcgca
     2221 tgacgcccca ggtgcacgtc actcgtccct tcgtggccgc cctggtggac gaggtcaccg
     2281 gtgctctgct cttcatcggc aaggtggcca agccggactg agcaatttca gcggcgctga
     2341 ttgagagcgg gtcttttcct tactaggtcc gcccagtcgg acagggctca agcattttcc
     2401 gcttgagccc tgtttccgca ccggcctgca ccagtggtct gatattactt ccctgacttt
     2461 tccacaatgt ttcttttgcg catcagctac gataaaacac agtagaagcg gggagcttcc
     2521 attcggcctg cccagatatg cgacccggaa gctaaaccga tatcggttga cctgcgataa
     2581 tgtccgggca tgtcggttgg atgccgctgg attgtcagga gtctcaagga cccattgaag
     2641 tttgatctga cgctggaccg tgaggcaaga gttgagctca gccagcagat ttacgagcaa
     2701 attaagacgg ccatactgag tggcaggctg aagcctggcg acaggctatc ttcttcccgg
     2761 gaactatcgg aggaactctc ggtctctcgt ccgacggtgg ccgttgcatt tgaacagctt
     2821 tcactggaag gttatattga aatgcgccat ggctcaggca cctatgtgca ggagggtttg
     2881 agcgcggccg ctgcggttaa gagaaagcgc ggtactgtcg gcaaagctac gcgtgggaga
     2941 agtaattccg acttcaggcg ggaagccgag cagatgagcc cttccactag aggtatggag
     3001 agtccggccc tttcctttca gtatggtatc ccggcactgg aagagtttcc caatgctgag
     3061 tggagtcgac tggtggggcg catctccaga aatgcctccg ccggcgatct agccatgaca
     3121 ggcgaggcct tcgggctcgt cacgctcagg catgccatag ctgattgcct ggtgcgtttt
     3181 cggggactct ccattaagcc tgagcaagtc atagtcgtgc agggtttgaa ccagggactg
     3241 gatcttgtcg ctcggctgca cctgaatccc ggcgccgtgg tggctatgga agatcctggc
     3301 tatcccctgg caccgcgtat ctttcgagcc aattcggggc gtgtggtacc ggttggtttg
     3361 gacgaccagg gactccatat cgagcaattg aagggctcta gtccggcact cctcaacaaa
     3421 gccaagctta tctacctgac tccttcccat cagtttccta cgggcatcac aatgtcccta
     3481 ccacgccgcc ttgagctatt ggcctgggcc aagcgctccg gggcaatcat tcttgaagac
     3541 gactttgata gtgaatacca gggcaccggt aagccgattc ctgcattgat gagccttgat
     3601 accaatgacc aggtgattta ttcaggcaca ttcaatcaac tgatgtttcc aggtatcggt
     3661 gtcggttact taatcgtgcc tgagagtctg gttcctcttt acgagagggg gcgcagactg
     3721 cttggtgagc ctgtcaattt gcaattacag cagtgtctgg ctgaattcat tttgcgcgga
     3781 gaattggaac ggcactggcg gcgtacccgg gtggtataca gtgaacgacg gcaggccttt
     3841 ctggatgcct tggagcgtta tacgagcggg ctttacactg ttcgcggcga gcagtgcggt
     3901 gtgttcgttt cggttacttt cgccggtcgc atgacgacgg aagcaataat caaaaaagct
     3961 gcccaaaaga atctcggctt gttttccacg cgagatttct acagtcggtc tgcagcgccg
     4021 gagcgtgagt tcattttcgg tttcggggct ctcaaggaaa cggcaatagt gaaaggcgtc
     4081 aagcttcttt cgacaattct ggcatgaatc aaaatagcgg caagctcgtt gcctggagcc
     4141 gaaccggctg aaggtagatt gtatctactg agccggagca gtggttgttg caggggcagt
     4201 gccggtggcg gcaggctttt tcttgcccaa tttactgaac aggctcttga cgcgattgtc
     4261 tctcaaaacg cggcgagttg cgccggtcat tctggtgaat acgtttttct tggcaggttt
     4321 gacggtagtc tgttgcggca gttgagcctg tgcttgcagg tcgcttgtga gcgcttgacc
     4381 gactgagagg agtgcgatca gggtgactat tctgcctttc attgttaact ccggaaaact
     4441 tgatggttgc ttattgtgga actgccgacc cgcagttgac tttgaggttg ggaaggtggc
     4501 ttcccatgta cttgaggggc gggttatcca gatgatttta gcagtagtaa gcgggccgtg
     4561 ggctctcgcg ggcgcagagg cagtttagtt ggtactgtgc gaactcgcgg tgctattggg
     4621 acattgaacc ggtatcgagt atctgtggag aatattcgat gccggtgctt gccggttcgg
     4681 gaagttgaaa tttgcaccgg aatgggcttc ggtagtcatt ttgcggaatc gctgcttttg
     4741 taacaatagt gatgtgaaga aatgttgcat gggctttcgc ggcttttcgc cgtcttgaaa
     4801 cccgcgcctt cagggcgttt gcgctgtcaa ttcgatttaa tttctgccgt tacatgagtt
     4861 tgtttggttt cctggcattt tcaaaacaga ccccctgaag ccggatctaa aacagatttt
     4921 cttcgcctcg gactaactaa aagaaatagt aagtattcgg gaaataagaa gtttccagaa
     4981 agcgcgtttc aagccggcaa aatccaagtc tggttgttat aggaacaggg ccgggtctaa
     5041 ggggcggact caaaacaggc gcgaaaagac cgtcagcagt ctccgggagt ggaaagttgc
     5101 tgacggcaga aatcttatgg ctccctagag gctacttttc ggcaatggga acttcccgca
     5161 agcggtcttc agaggcgata gaggtgatgc agccgaccga agatatccag cgttaccggg
     5221 tggttgttgc ccaggtgctt ttccagaatg gtgagagcct cgacgaaggt gtcgcgggcc
     5281 cggatttctt cgcccatgga ctcctgaagt tgagccaggt cgttgagggc atagccgacg
     5341 gcctgatgct ccgcgcccag ttgctctttg gcaagggtga ttgcgtcgac gtaaaagcct
     5401 tccgcctttt caaattcctg ccagcgctgg tggtcggagc cctgcttgag caggttggat
     5461 acttcgagga gaatcgggca ggtgggggga gtctggttca tactgtcttc cttttgattg
     5521 tgcttcagag aagccgttcc caggcagcgg ttgccgccct ggacagagtt gcgattgtca
     5581 aagaaatcct tgcgttctcg ctctttcagg cagaaaaaac accgcgtcgg cagccgcaac
     5641 acttgagagt gagggctttg gcttggaaat gggcgggggg cgctggtaag cgatttagtt
     5701 agtgatacct gaaaagaaag gagaaagaag gatgcttact gttctagcgg aggcgggctc
     5761 cacttcaaag cacttttgtg aaggcagatt acgagcgatc gttgctggtt cgcaacattt
     5821 gcctttcggt tagttctgca atgtggcgct tttcggctgt tagaaagggc atccaattag
     5881 catttgccag tttgttggat accccataaa tgtgcttggt ctcgggctca gacaattgcc
     5941 tcccgagcat tatggctttg ctaagattgt cgggcacgct caaaatgcgc ctagcgcaat
     6001 tacgcaatcc cagtgcaagc cggaagccgg atacgaaccg gagaacttgt gagaagcgat
     6061 gtctgccgtc attgaagtgc gaaacctggc taagaagttc gacaaggcga ttgccgtgga
     6121 ggatatctcc tttaccatcg agaaggggga attcgtgggg cttctgggcc agaacggcgc
     6181 cggcaaatca actacgattc agatacttct gggcttgatt acgcccacat ccggtgatgt
     6241 tcgtatattt ggctacagcc cctggaagga gcgagtaaag gttttagagc gcagtaattt
     6301 cagttcggct tatgtagcca tgccgcccaa tctacaagta cgggagaacc tggaagtttt
     6361 tgccagactt tatggcgtgc gcaatcgctc ccgcaagatt gatgagcttc tctccttgtt
     6421 cgccgtggat catttagcgc gaaagatgac cggagccctc tcttccggcg aatctacgcg
     6481 ggtgaacctc tgcaaagcct tattgaacga cccggagctc ttgcttctgg acgaaccgac
     6541 tgccagcctt gatcctgata ttgccgacaa ggtgcggggc ctgttgcgtc agattcaaaa
     6601 ggaaaggggg ctcaccgtgg tctacacctc gcacaatatg accgacgttg aggaactctg
     6661 ccaccgcgtg ctgttcttgc accgggggcg aataattgcc cagggtactc ccggtgagat
     6721 agttgagaat ctgggtgagt cgtcactgga ggaggttttc atagccatcg ccagagacac
     6781 cctgaaggtt gctcctggtc cgtcctctgt tcccggaact gctcctgatg ttgcgcaagc
     6841 ttctgcttcg gcccggaacg atggcgagag taaaagcgag gatgcgtcgg tggaaatctc
     6901 tggaaggccg ggacgggaga aggaagagct atgctgacag ttcgcccgga ccgggtcgcc
     6961 ggcctttgtt accgttatta tcttgtgctt tcccgcagca gtttcagggt catggatata
     7021 gtcttctggc cggtaatgga ccttcttgtt tggggcttcg tcagtaacta cttctccagc
     7081 ctgggtggtt cggcaccaca ggcagtgacc tttctggtgg gtggaatcat ctttttcaat
     7141 gtcctctata gggcgcagca gtctatatcg gtgtcatttt tggaagacct gtggtctcgc
     7201 aatctgctca gtatttttgt ttcaccgatc acggtagcag agtttgtcag cgctacctat
     7261 gtggtaggag tggctcaggc actggctgtc acgctcataa tgtctgttat ggctgcgttt
     7321 ctttatgcct ttaatatgct cagtctttcc tggtatttga taccactttt cgtcaacctg
     7381 ctggtaatgg gctggtggct ggggcttggc accacggcct tgatattgcg ctatggctat
     7441 caggcggagg ccctggcatg ggctgtgccc tttctgttgc agcccgtctg tgccgtcttt
     7501 tatccggttt cggttttgcc cgcctggctt cagtcggtgg cctggtttgt gccggctacc
     7561 catgtctttg agggcatgcg ccatatcctt tccggcggca atcagtgcgc tgtttatttg
     7621 atcaaggctt cccttctgaa tgttctttat tgggtggcta ctacaatttt cttcggggcg
     7681 atgataaaca gggccaggga gcgcggctcc ctggccaaga tagtcactta gttgctttcc
     7741 tttcctggaa atggaaacag ttgtcgttcc ttatgttaac ggcgtaaagc acactttcag
     7801 ctgtgccgag aagattttat tttgggcgtg aaagcctgcc ggttgcaggt ttgtctgttg
     7861 tggtggcgaa gatggtggcg cttgtggtgt tgacagaact gttttggaag cgtatcaatg
     7921 tctgtagaac cgcatggcgg tggcttttga agatgctttg ttttgaaaag cattatcgtt
     7981 tttaaaaagc cgagttcaaa aaacatagta ttgattttca ttttcaggag atttcaaatg
     8041 acttgcggaa gcgaacataa aggacatgac cataaacacg gagaaggttg tggtcacaag
     8101 acagtcgagc atgatggtca taaagactat cttcatgacg gtcacctcca taacgtacat
     8161 ggcgatcatg tacacgatca ctctttgagc gttggcggtg ccaatgctgc aaactgcaca
     8221 cctgagcaca agtgcggcgg ccacgacggc ggtcacaaac atgctgccgg ctgtggtcac
     8281 gaagccgtgc cccacggtga tcacgttgat tacctggtgg aaggtcatct gcaccatccc
     8341 tgcggcagcc actgtgatga tcacggtcct gtcaacgtca agtaggaagc gcaacggtat
     8401 ttattcaact gttggtcaaa ggggtgagcc aggcggttca ccccttttgt cacggcgttt
     8461 ttccagcacg cgctgctctt aattcggacc tatcgtttgg tggccatgat ggcgtcagag
     8521 agagttttgc gcgataggca gtaatagcgg tctgcttccg attctctccg catgcggctg
     8581 aggagcacac cgtggtcttt gtatatggct gccagtcggc gtttgagcag actgcccagg
     8641 gtgcattgtt cgccttcgtg ggaggaaagc agcaggtagc ctctcagcga tgccgtctgc
     8701 atg
//
LOCUS       DAOQNZ010000004         8116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3285_length_8116_cov_3.847786, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000004 DAOQNZ010000000
VERSION     DAOQNZ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3285_length_8116_cov_3.847786"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2481)
                     /locus_tag="PKW73_00150"
     CDS             complement(<1..2481)
                     /locus_tag="PKW73_00150"
                     /inference="COORDINATES: protein motif:HMM:NF040119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6493 family protein"
                     /protein_id="HNA71716.1"
                     /translation="MAEVKELEATLEHLILNGSVEELREFLTTTEARAQRSKLSSLAR
                     TWHKKADGGAFEQDGDTYRFKADYPPHVSGNAFEAVVLTASAGEFKGIKWHAGEFSCL
                     TETTARLLAPDCLNACVESLLIDNPRAYRGVRTLVSLGLCARPKIEQYGIGLIAYKGY
                     HEQRDAFIAELRRDPSILKEDIWLLFEVEGSGEISLAAMDKFSRPGHLWSDVLYCLQE
                     DGSLSRDRLLKASLEALNRGFIQFRAGWFSRFHEVLKPTESERSLLVEDYGRLLANNI
                     PPTVSFALSAWLTIDKAKPIESEMVERYLPPCFASQTKSTLLAALSLVERVCKRDKAF
                     RKPCLKLALQALAQENAEVQGRLLKLLKAHQDVVDEEITGLIEQQMAAITPPLVDEFR
                     SILGNTAEPEMEICEVYSMADGACESFHMGTPLPVIESPEEFIEAALTIIETPFNPAS
                     VDVILKAVALGKVEALRNIGRSEDGSLLAKMAAPLIARLKKVEKQQAESWNKGAFYFF
                     LHFLLHFLGADTIADDHREKPLEYFRTLDDGAIEKISFCWTIRHFAERLLGILERTAS
                     GVFISAGLPSLDSGYVSAEEFLQQAKEASSVEFAFMEREPYLALMRLPWCAHDQVLKE
                     LGGKGYSGKYVEKLKTILRQLVLVRQKHAGADPCERSLLIVKDLRESSDKGMMEMAHS
                     PAFKSRDESIIQLAFYNYPILCEEFHERGISECQSVVRWFEAADLALRFYFLPMALGF
                     APLQDRANLLLATGCLLSAPEVKSLVTDCLIGSIEEKRLSPDRLGEKLGLLLHMEISM
                     PKRVATILSEVARVSSLHNDAVRQILEYS"
     gene            complement(2528..4012)
                     /locus_tag="PKW73_00155"
     CDS             complement(2528..4012)
                     /locus_tag="PKW73_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HNA71717.1"
                     /translation="MSESKSALAINQTISYGFDSNLVSTGPSGRSHLTLATSIREARY
                     PYFFEGRVKEARSVARALLTLSQVVGARFYTPPNMLKRIMAERDPVITSGAGALRFEG
                     FSACASAYARVDLLPGAFDGDVVERGTTNVDFNAALKAALASTMSTDALDVSVGKEEF
                     ALAKGGAPVIEKKVRLPLRWLKGFVEVQAYQARMEKKLTVGKSEALSFLRGLPQNAHN
                     QSLFYVVPSGRGLRLSQVAAGGGSSAPVQVGGLKRLQLLRELVPLIANSQKAAMTVYA
                     SPDGQASEWKLYLDQGLVFSLSMTAQPFRGFSGEGQVLADLTEVQEEKLAGLRAALKW
                     QQNLSLHDLSGATDTSADELKTALAVLGSRGLVGYDLDFGTYFHRELPFDLSLVENMH
                     PRLKAAQKLLAEGGVRLLDAEADADGDTKGDAAEDRLRLEVKGSDVMHVVTLSPLDSS
                     SRCTCQWFSKYQGARGVCKHILAARLKLAVDEGDGGEEESDEAD"
     gene            complement(4065..4487)
                     /locus_tag="PKW73_00160"
     CDS             complement(4065..4487)
                     /locus_tag="PKW73_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71718.1"
                     /translation="MGDWGARPWDNDEGADWFLLFNESSNFDQIIGQTIAEPYDGCNR
                     DTLRAAICTFILLGRVYVYPPGSYFAHLDLCIERLKELKQAWFDGEWDGDSEIGKDID
                     FSLQYLERRKACIGKKAEEMPHLSEELLRWLTALLEAC"
     gene            complement(4565..4801)
                     /locus_tag="PKW73_00165"
     CDS             complement(4565..4801)
                     /locus_tag="PKW73_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71719.1"
                     /translation="MYNLDFGCAAGTASAPAVDDFDGSVRTCGLDGKQEFLQEVRLII
                     SAGRVPSREIEKPEETSVTCSNGVCAVTWKPVRK"
     gene            5418..6674
                     /locus_tag="PKW73_00170"
     CDS             5418..6674
                     /locus_tag="PKW73_00170"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNA71720.1"
                     /translation="MPGPYSAALTINQPAGFKQRFFAAVIDEFILGVVVFVVSLCGLI
                     PVGQYFLTGGGGLTNPGQIPFPYMLHLGGAFAVVILANTIPYLYIALFEASKLAATPG
                     KWAMGLKVVSDSNSNVSLRDGISKTLTQTLLYLPIFTLIGLTILTTLSFCFSKNAASP
                     DKDKLDYLSCILSLIAALVTTAVLSFPCFRKGTQSLTDLICKRFVVERETPNKVSIPS
                     LQNADAAIYKLHHVTAWVFWVPLTILVTAIELAIFLWGPLKLFGDFISAIVVMVLCWI
                     GSLWLLWKFRRQAGDLLAKAITGLLCVTLGTGMIIMAPEYITRVSNTPKVLQLMDQAI
                     QANDRGDQKNYEKFRLQARSIPSYAIAFYIMRHDFYEKPLGMAQEAYMAPRMWGITSN
                     PGIPAKEKPGSKEKNESSGKSLPKEE"
     gene            6719..7486
                     /locus_tag="PKW73_00175"
     CDS             6719..7486
                     /locus_tag="PKW73_00175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA71721.1"
                     /translation="MTSKTQSTGWFAHFFQGAALELWRRANSIEDTQREVNFLEEALA
                     APVGGKLLDLPCGNGRLSIPMALLGYDVTGVDFSKTFVEEARSAAKAYKCKTTFVKAD
                     MRELTYENEFDGAFCMGNSFGYFDRAGTNTFLKTVSNCLKPGARFVIESSMIAECFLV
                     NGGEREWLQRGDMYMLIENKYDCRNSTVETKYVFVQNGKEETRTATHWVYTTGELCQM
                     LEVAGFAVEELFSSPDPDEPEPFTLGAERLLLVAEKK"
     gene            complement(7604..>8116)
                     /locus_tag="PKW73_00180"
     CDS             complement(7604..>8116)
                     /locus_tag="PKW73_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71722.1"
                     /translation="LTACARVFQTSSSLSEYQAPVAGQSDVTLAEVVVNNCPPPKEGK
                     TVVELGIIVSADGRVDYTARHMELMSPLPLSVREGERAPYRNVLDELMDYRCQRAPGS
                     EARVARLARKMNIAAELVPKTLRELGYLPDDIASGVAVEHMLKRLKRSKSSNRGVHID
                     DGDGPATCTG"
BASE COUNT         2067 a         2319 c         1871 g         1859 t
ORIGIN      
        1 gctatactca agaatttgcc tgacggcatc gttatgcaaa ctgctaactc tagccacttc
       61 gctcagtatt gtcgccactc gtttaggcat actgatttcc atatgcaaga gcagccccag
      121 tttttctccc agacgatcag gactgagcct tttttcctca atactcccga tcaaacagtc
      181 agtcaccagg gatttcacct ccggggcaga aagcaagcag ccagtggcaa gcaaaagatt
      241 agccctgtcc tgcaagggtg caaaacccag agccatcggc aggaagtaaa atctcagagc
      301 caaatcggcc gcttcaaacc agcggactac gctctggcac tcacttatgc ccctctcatg
      361 aaactcttca caaagaatcg gatagttgta gaacgcaagc tggataatac tctcatcgcg
      421 acttttgaat gcgggcgaat gagccatctc catcatacct ttgtcactac tctctcgtag
      481 atccttgacg atcaagagac tgcgctcaca gggatcggca ccggcatgct tctgcctgac
      541 caatacaagc tgccgcaata ttgtcttgag cttctcaaca tacttaccgc tatagccttt
      601 accgccaagc tccttcaata cctgatcatg agcacaccag ggcaaccgca ttaaagccaa
      661 atacggttct ctttccataa aggcgaattc aacagacgat gcttcctttg cctgctgtaa
      721 aaactcttcc gcactgacat agccgctatc cagactgggc aatccagcac tgatgaaaac
      781 accgctggca gttctttcca agataccaag cagtcgctcc gcaaagtgtc ttatcgtcca
      841 gcaaaacgag attttctcta tagctccatc atctagagtc ctaaaatact ccaacggctt
      901 ttctctgtga tcgtctgcaa tggtatcggc acccaaaaag tgcaacaaga aatgcaagaa
      961 gaaatagaag gcacccttat tccaagactc tgcctgttgt ttctcaacct tcttcagcct
     1021 ggcaatcagc ggagcagcca ttttagctag aagagaacca tcctctgatc tgcctatgtt
     1081 gcgcagagcc tcaaccttac ctagcgccac cgctttcagt atcacgtcta cggaggcagg
     1141 gttgaaaggt gtttcaatta tagttagagc agcttcaatg aactcctcag ggctttcgat
     1201 cacgggcagt ggtgtaccca tgtgaaaact ctcacaggcg ccatccgcca tagaatagac
     1261 ttcgcagatt tccatctccg gttctgcggt attgccaaga atgcttctaa actcatccac
     1321 taatggcgga gttatcgcag ccatctgctg ctctataagt ccggtaattt cttcatccac
     1381 aacatcctgg tgagctttca gtagtttcaa aaggcgcccc tgcacttcgg cattttcctg
     1441 agcgagagcc tgcaaagcca atttcaagca aggcttacga aaggctttat ctcttttgca
     1501 gaccctctca actagcgaca aagcagcaag cagggtactc ttggtttgag aagcaaaaca
     1561 gggcggcagg taacgctcta ccatctcgct ttcgataggc ttggccttgt cgatggtcag
     1621 ccacgctgaa agggcgaaag agaccgtagg tggaatatta ttcgcaagga gccgtccgta
     1681 gtcttccacg agtagtgagc gctcgctctc agtcggtttc aaaacttcgt gaaagcgcga
     1741 gaaccagccc gctctgaatt gaataaaccc tctattcaag gcttccagac tcgctttcaa
     1801 gagcctatct cgggaaagac tgccgtcctc ctgtagacaa tatagaacat cgctccacag
     1861 atgccccggt ctggagaact tatccattgc cgccagactg atttcgccgc tgccctccac
     1921 ttcaaataga agccaaatgt cttccttcaa tatgctcggg tcgcgacgaa gctcagcaat
     1981 gaaagcatct cgctgctcgt ggtatccctt gtatgcaatc aaaccgattc catattgctc
     2041 tattttgggt ctggcacaaa gacccaggct gaccagagtt ctcacacctc gataagctct
     2101 tggattatca atcaagagac tttccacaca ggcattcagg cagtcagggg caagcagtct
     2161 ggcagtggtc tctgtcagac aagaaaattc accggcatgc cacttgatgc ctttaaactc
     2221 accggcagag gccgtgagaa ccacagcctc gaaagcattg ccagagacgt gagggggata
     2281 atcagcctta aatctgtaag tatcgccgtc ttgctcaaaa gcccctccat ctgccttctt
     2341 gtgccaggtc ctggctaacg aagaaagctt ggaccgctga gcccgagcct cagttgtggt
     2401 caggaactct ctcagttcct caaccgagcc gttgagaata agatgttcaa gggttgcttc
     2461 cagctcttta acttccgcca cattcgtctc tctttcttga tcagattgtc acgccttcca
     2521 aatcttctta gtcagcttcg tcgctctctt cctcgccgcc atcgccttca tcaacagcca
     2581 gcttcagcct ggctgccagt atatgcttgc aaacaccacg agccccctga tacttactga
     2641 accactgaca ggtgcagcgt gaagaactat ccagtggaga taatgtcacc acatgcatga
     2701 catcactgcc tttcacttcc agtcgcagtc ggtcctctgc ggcatcaccc ttcgtatcgc
     2761 catcggcatc cgcttcggca tcaagcaacc tgacgccgcc ctcagccaga agcttctgtg
     2821 ccgccttcaa gcgaggatgc atgttttcca caagactcag gtcaaagggc agctcgcggt
     2881 ggaagtaagt accgaaatca agatcgtagc cgaccagacc gcgactacca agaactgcca
     2941 gggcggtctt aagctcatcg gcactggtat cagtggcgcc ggagagatca tgcaaactca
     3001 gattctgctg ccacttaaga gccgcccgca gcccagccaa cttttcttcc tgcacttccg
     3061 tcaggtcagc cagtacctgc ccctccccgg aaaagccacg gaaaggttga gcagtcatag
     3121 agagcgagaa caccagcccc tggtctaagt aaagcttcca ctcgctggcc tgcccatccg
     3181 gactggcata aacggtcatg gcggcttttt gagagttggc aatcagaggc acaagctctc
     3241 tcagtagctg caaacgcttg agaccgccca cctgcaccgg agctgaactg ccgccaccgg
     3301 cagccacctg gctcaagcgc aggccccgac cggagggcac cacgtagaaa agcgactggt
     3361 tatgagcatt ttgcggtagc cccctgagaa aactgagggc ttcgctcttt ccaaccgtaa
     3421 gtttcttttc catgcgagcc tgataggcct gcacctcaac aaaacctttc agccagcgca
     3481 gcggcagccg caccttcttc tcgatgacag gcgcgccacc tttggcaaga gcaaactcct
     3541 ccttcccaac agagacgtcc agcgcatcgg tgctcatagt ggaagcaaga gcagctttca
     3601 aagcggcgtt gaagtcaaca ttggttgtgc ctctttctac aacatcgccg tcaaaagcac
     3661 ccggtaaaag gtccacacgg gcataggcac tggcacaagc gctgaagccc tcaaagcgca
     3721 gggcgccggc accgctggtg ataacagggt cacgctcagc catgatgcgt ttcagcatat
     3781 tgggcggcgt gtaaaaacgc gcaccgacaa cttgagagag cgtcaaaagg gcccgcgcca
     3841 cactgcgcgc ctctttcact cgcccctcaa agaaataggg atagcgggct tcccggatgg
     3901 aagtcgcgag agtcagatgc gatcgaccgg agggaccggt gctcaccagg ttggaatcaa
     3961 acccatagga aatagtttga ttgatagcaa gtgcgctctt gctttcgctc attgaaattt
     4021 cacctgtagc cgaagtaagg tggaactgcc gaagccttca caattcaaca tgcctccaaa
     4081 agtgcggtga gccaccttaa cagttcctct gacaggtgag gcatttcttc agctttcttg
     4141 ccaatgcaag ctttccgccg ctctaaatac tgcagggaaa agtcaatgtc tttcccgatc
     4201 tccgaatctc catcccattc cccatcaaac caggcttgct tgagttcttt tagacgctcg
     4261 atgcaaagat ccaaatgcgc gaagtaagaa ccaggcgggt aaacatacac tcttcccaag
     4321 agtatgaaag tgcaaatggc agcccgaagc gtatctcgat tgcagccatc atagggctcg
     4381 gcaattgtct gacctatgat ctgatcgaaa ttagaagact cattgaagag aagaaaccag
     4441 tcagcccctt catcattgtc ccagggtctg gccccccaat ctcccactgt agaatctccc
     4501 caatcagcga caatcactcc ctcaatttac cccgctggag gagaaccaac attaaggaac
     4561 cccttcactt ccggaccggc ttccaggtga cggcacaaac accgttggaa caagttactg
     4621 aagtttcttc aggtttctcg atttccctgc ttggaactct tccggcagag ataatcagcc
     4681 ttacttcctg aagaaattcc tgcttcccgt ccagaccaca ggtccggaca ctgccgtcaa
     4741 aatcgtccac agccggggct gaggcggtgc cagcggcaca gccgaaatcc aggttataca
     4801 tcttagagac cctcaaagta cgagtaagcg gagcgttttg gagaatgagc cgggctttct
     4861 gtttgcggat ttaaaccgtc ggcgccttct tccttcaggc tcactcaaca ttctaattgt
     4921 cctattttgg catcttccaa accatcagcg catcacccta ttcttgccgc agacagaccg
     4981 atctgaaaat acttgtttat cggtcaacgg gccgataaac gctggcaaaa tctatagaga
     5041 aacccgccaa actgctaaaa agcgggcata ttgcactgga atactggaat accggcaggc
     5101 tggcagaccg gcacccggca gcaccaatta gtcagccctt cagaaagaga attcacctgg
     5161 ctatggacca cgaaaagctc gccgacctgc aagccaataa gttcaaattg actgtaacct
     5221 gctcaaattg caatacggta tttgaatcca aagaaattac ttgcccaaaa tgccaaagca
     5281 caattaccac cgagacacta tcgacattta agagcacagg caaagatacc ggttcagatt
     5341 ctcattctgg gcgagcacca gtggttgtac cctcggcaga tgagtacgcc agtgggtatg
     5401 cattgcaacc gaaccagatg cctggaccgt actccgcggc ccttaccata aatcaaccag
     5461 ccggcttcaa acagcgtttt tttgctgccg tcatcgacga attcatcctc ggcgtcgtgg
     5521 tcttcgtcgt ctccctgtgc ggattgatac cggtgggaca gtacttcttg accggcggcg
     5581 gaggcttgac aaacccaggt cagataccat tcccatacat gctgcacctg ggcggtgcat
     5641 ttgcagtcgt cattctggca aacactattc cttatcttta catagcactc tttgaagcga
     5701 gcaagctggc agccacacca ggcaaatggg ccatgggatt gaaagtggta tcagacagca
     5761 acagcaatgt aagcctgcgc gatggtatat ccaaaacgct gacccagaca cttctgtatc
     5821 ttcctatttt cactctcatc ggacttacca tcctgacgac actttccttt tgtttcagca
     5881 agaacgccgc cagccccgac aaagataaac ttgattatct atcttgcata ctctcgctca
     5941 tcgccgcact agtaaccaca gccgtactca gttttccctg ctttcgaaag ggcacccaga
     6001 gcttaaccga cttgatatgc aagcgctttg tggtggagcg cgagaccccc aacaaggtat
     6061 ccataccgtc acttcagaat gcggatgcgg ccatttacaa actgcatcat gtgaccgcct
     6121 gggtcttctg ggtgccgctc acgattctgg taactgccat tgagctagcc atattcttat
     6181 ggggaccgtt aaagctattt ggagacttca tcagcgctat agtcgttatg gtgctctgct
     6241 ggataggcag cctctggctg ctctggaaat ttcgccgaca ggcgggcgac ctattggcta
     6301 aagcaatcac gggtctgctc tgtgtgacac tcggcacagg catgatcatc atggcaccgg
     6361 aatatataac gagagtttct aataccccca aagttctgca actgatggac caggccatcc
     6421 aagccaatga caggggcgac caaaagaact atgaaaagtt tcgcctgcaa gcccgaagca
     6481 ttccgtcgta tgccattgct ttctacatca tgcgccacga cttttacgag aaaccactgg
     6541 ggatggcgca ggaagcttac atggcgccga gaatgtgggg tatcaccagt aatccaggca
     6601 tccccgccaa agaaaagcct gggtcaaaag aaaagaatga atccagcggg aagagtctgc
     6661 caaaagaaga gtgaatttcg agtacactgg ttgagttttt gcagcagaga taaacttcat
     6721 gacctccaaa actcaaagca ccggctggtt cgctcacttc tttcagggcg ccgcacttga
     6781 actgtggcga cgcgccaatt caatcgaaga cacccagcgg gaagtgaatt tcctggaaga
     6841 agcactggca gccccggttg gcggcaagct tctcgacctg ccctgcggaa acggcaggtt
     6901 gagcataccc atggccttac ttggttatga tgtcaccggc gtagactttt ccaaaacttt
     6961 tgtggaagaa gcccgctcgg cagctaaagc ttacaagtgc aaaactacct tcgttaaagc
     7021 cgatatgcgc gaactcacat atgaaaacga gtttgatgga gccttctgca tgggcaatag
     7081 cttcgggtat ttcgaccgag ccggtaccaa cacttttctc aaaaccgtga gcaactgcct
     7141 gaaaccggga gcacgttttg tcattgaatc atccatgatc gccgaatgct tcctggtcaa
     7201 tggcggagag agagaatggc tgcagcgcgg cgacatgtat atgctaatcg aaaacaaata
     7261 tgactgccgc aacagtaccg tcgagaccaa atatgtcttt gtccagaacg gcaaagaaga
     7321 aactcgcaca gccacgcact gggtttatac gaccggcgaa ctctgccaga tgttggaagt
     7381 agccggcttc gccgttgaag aactcttttc ttcacccgac ccagacgagc cggaaccctt
     7441 cacccttggt gcagaacgcc tgctactggt ggcggagaag aagtgaacta gttatcttct
     7501 catcacctca ggctttccct ttgaaatatt aagactgagc gattttcgac aaaatttcgc
     7561 cttcgcctca actcttaaaa caagcatccc cgggctacag actctagccc gtgcaagtgg
     7621 ctgggccatc tccgtcatcg atatggacgc ctctgttgct actcttgctt cgtttcaagc
     7681 gcttgagcat atgttctaca gccacaccac tggcaatatc atcgggaaga tagcccagct
     7741 ctcgtaaggt ctttggcact agttcggctg ctatattcat tttgcgagcc aggcgggcca
     7801 cccgcgcctc actgcctggt gcgcgctggc agcgatagtc catcaactca tcaagaacgt
     7861 tacgataggg agcccgttcc ccttcacgca cgctcaaggg cagggggctc ataagctcca
     7921 tatggcgagc cgtataatcg acccggccgt cggcactgac aataataccc agctctacca
     7981 ctgtcttgcc ttctttcggg ggtggacagt tgttcaccac tacctcggcc agagttacat
     8041 ctgactgacc ggctaccggg gcctggtatt ctgacagcga tgaactggtc tgaaagaccc
     8101 tggcacaggc ggtcag
//
LOCUS       DAOQNZ010000005         8062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3313_length_8062_cov_3.978144, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000005 DAOQNZ010000000
VERSION     DAOQNZ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3313_length_8062_cov_3.978144"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKW73_00185"
     CDS             <1..787
                     /locus_tag="PKW73_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71723.1"
                     /translation="SEDVRLGPARGNIQVPVGSRIKLEGNHSLIESLDPLKELPGQDF
                     IALELTRLPITNAQLKDLNRLTGIQHLSLENTDVDDGVLPYLADLKDLRYLNLRRTMI
                     TGRLLPELKRWPKLNRLKIGHNALRKEFINRACFACLKELPRLEELQVSSAGLDDESL
                     KGIAEIKQLHSLDISGNQKVSEVGLSYLASMPRLYYLNLGDIKLPARAVCKLGRCGVH
                     SLVLSFRFYTPAELKTIKTCLPGRKIANSVKNNVPLEMYAPLH"
     gene            1029..2537
                     /gene="tldD"
                     /locus_tag="PKW73_00190"
     CDS             1029..2537
                     /gene="tldD"
                     /locus_tag="PKW73_00190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNA71724.1"
                     /translation="MVNTFERPLSEAALLHSCIFSPETELHNAFGLTYGQLQKIIADA
                     SKGSGGKNGFCDVFLQAGSAQSLTLDQEVIKSATSSHVNGAGVRVVVGDKSGYSHTDH
                     VTVANLKRCATIARAISEHAQESANLALMENQKAVQKPHNLYQISQSPASWDLKDKIA
                     LLRKMDQMARAYDPRISNVSVSLGVEDYTIIIATSRGDIFADKRPLVRLNISCLAEDG
                     SKRENGSSGGGGRFDFQQLEDEAYWQEHVLIAAKQAIELLNADPAPAGEMDVVLGAGW
                     PGVLIHEAVGHGLEGDFNRKGTSAFSSLMGQSVASKLVTVIDDGTIAERRGSLNMDDE
                     GTPTGRTVLIENGKLTGYLQDRQNASLMKAGLTGNGRRQDYRFAPIPRMTNTYMAGGT
                     SSPDEIIASVKRGIYATTFGGGQVDITNGDFTFSATCAYLIEDGKLTRPIKGATLIGN
                     GPKALHQVAMVGNDVSLDKGIGTCGKDGQGVPVGVGMPTIRLNGITVGGSKS"
     gene            2576..3904
                     /locus_tag="PKW73_00195"
     CDS             2576..3904
                     /locus_tag="PKW73_00195"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNA71725.1"
                     /translation="MATAKLEDIAAAIVHQARKLGAAECDVTITTRNAVETSVRLGQV
                     EKLEGAQMRGLQFRALCGAKGTKTASSSTSDFGAKALKQLVKDTIATAEASEEDPYAG
                     LPEPGDLAREYPDLGLFDPAVEKIPVETKISLAKEAEEVARGADPRITNSEGASFSDS
                     AGQIVHANSRGFLGSYHGSHVSLSVGVVASQDDEMQVGGWWASNRSFDKLESAPAIGL
                     KAAERALRQLGSRKVKSQEVPVVFDPLMAARLIAQFIGAAAGSHIYRKSSFLVDKLGQ
                     VVASQGVNIIDDPLMAGRLGSHPFDSEGLPARTRTIIEEGRLVTYLLSSYAARRLHTR
                     PNSGSLGNLYLQAGKESPEEIIGSVKNGLYLTSVSGPGFKVITGDYSLGASGIWIEDG
                     KLAYPVSGITIASDMLSMFAGIEAIGNDLVFRSSTASPTIKIGKMTVAGQ"
     gene            4046..5797
                     /locus_tag="PKW73_00200"
     CDS             4046..5797
                     /locus_tag="PKW73_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71726.1"
                     /translation="MEVAVVARNLIGAKRFQEALQLTGDKIKVMENDRSSHISPGNWR
                     LDGVGHALAFQMLTQPQLMEIYLGDIYNLHGLCLYQLKRIPESLKYVQLALDHNPYQA
                     QALQNKGVILAKMGKHDEAIKCFDQLLRQNERFVGAYLNRAEIYKIKKLYSLEQKDLD
                     NYHKFKILNQKQELNETILSRFEYAYHIIKGKPRSPEQLTALGIIHWENLRLKESEES
                     FKQAIQLNPKYAPAHLGYSIFLEWMKRHKDAIRESSLALSLDKNIPIAYITRARARIA
                     ENQLPGAIDDYNTLLNTEKSDTGLTIRALGNRAACYAKLARYKEAIEDVNHALSLRPA
                     AATKAELLANRGLCYEKLGDKQQALKDYDSALAANPGERRAYENRGKIMLSMGQYEQA
                     MADLNTSGSAAAEASPSLPTGKELSSMVAHYDKLLKLFPNSTESLYNRGLLQLTLGKP
                     ALAAQDLQKVVSLSKQANNTSDYAVCFANIALRLDGQKEKAKELINQYRLLKRQDLAS
                     PVVDYFIKGKTSVLTTATSNSGNRAGQTRSLTLLGLDAYASGDKVQAREYLSKVRHEG
                     DTSTDEFTLALSYWNKL"
     gene            complement(5815..6909)
                     /locus_tag="PKW73_00205"
     CDS             complement(5815..6909)
                     /locus_tag="PKW73_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71727.1"
                     /translation="MSEDGKLKQIVPYERKGGGRQVFLAVFWWLYMALCFACVSAALI
                     GSLYAGEWLSSVVSLTLFLVCLYLFSGYLILSPVIFYSVRLLSRGQLHDAEKVLNRGL
                     NMVRRLGLRKDWSYMTAVSNLAVVKLASGQFAESELIYGELMERLGREKKIAKNALAA
                     IYVNNLAYAHLRQGELEEAEANSLKALEIWSKLKGNEALGQAFPLVNLAEIDVLRGSF
                     TAAESKLERALAFVHGSGRPLAVMEESFHSLDIHIRIFQAIICLETGRREDAVRLSES
                     VLASMQQAFKPPAGYNLTSLARLAELLLQAGDEEMAERVLEHAYDQARDFPTHPDAPH
                     ILDIYERLLTASKRVHELPDLKAWVRPVLL"
     gene            complement(7188..7454)
                     /locus_tag="PKW73_00210"
     CDS             complement(7188..7454)
                     /locus_tag="PKW73_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71728.1"
                     /translation="MNLMQLVLLVLFSLVGSYAGRYLQGMFAGEISRISSNGMRNLAS
                     ALVYALGNSVVVFLLASLAGIGISLLFWFILILAFEFFFNGMRG"
BASE COUNT         2143 a         2356 c         2018 g         1545 t
ORIGIN      
        1 cagtgaggat gtgcgactgg gaccggctcg cggcaacatt caagttcctg tcggctctcg
       61 cataaaattg gagggcaacc actcactgat cgaaagcctc gacccgctca aagaactgcc
      121 cggccaagat ttcatcgccc tggaattgac gcgcctgccc attacgaacg cacagctcaa
      181 ggacttgaac cgccttaccg gtattcagca tttgtccctg gaaaacaccg acgttgatga
      241 tggagtgctt ccctacctgg ccgacctgaa agatctgaga tatctaaatt tgcgacgcac
      301 catgatcaca ggtcgcctcc tgcccgagct caaacgctgg cccaaactca atcgcctcaa
      361 aattggtcac aatgctctcc gcaaagagtt tatcaatcgc gcttgcttcg cctgcttaaa
      421 agaactgccc agactggaag agttacaagt ctccagtgcc ggactcgatg atgaaagctt
      481 gaaaggaata gcagaaatca aacaactcca ctccctggat ataagcggca atcagaaggt
      541 gagcgaagtg gggctctcct acctggcttc catgcccaga ctttattact taaacttagg
      601 cgacatcaaa ctccccgccc gggccgtctg caaactgggg cggtgcggcg tacacagcct
      661 ggtgctcagt tttcgctttt acaccccggc cgaactgaag accatcaaaa cctgcctgcc
      721 cggcagaaaa atcgccaact ccgtcaaaaa caacgtgccc ctggaaatgt acgcgcctct
      781 gcactagtaa aaccgggact ttcgccgtga cccgaaaatg ccataaacgt gacctattat
      841 gaacccatca aaagcccagc ggccattgaa acacttcctc tgcctccccc catgcggtta
      901 aatcgggtcc ggtctacgac ccgatttaac tttttcagtt tccaagctcc aaatcctgcg
      961 gccaacatac tacttatcaa gctcagccgg tataaaatag gctttgcact caagatgagg
     1021 ttggaaacat ggtcaacact ttcgagcgcc cgctttccga ggcagcgctt ctccactcct
     1081 gtatcttcag ccctgagacg gaactgcata atgccttcgg tctcacctac ggtcagttgc
     1141 aaaaaatcat tgccgatgcc agcaaaggct ccggcggcaa aaacggcttc tgcgacgtct
     1201 tcctgcaggc cggctcagcc cagtctctca ccctcgacca ggaagtgatc aaaagtgcca
     1261 ccagctccca tgtgaacgga gccggtgtgc gagtggtggt cggcgacaaa agcggctact
     1321 cccacaccga ccatgtcacc gtggccaacc tgaaacgctg cgccaccatt gccagagcca
     1381 tttcagaaca cgctcaagaa agcgccaacc tggccctgat ggaaaatcaa aaggctgtcc
     1441 agaagccgca caatctctat caaatcagcc agtcaccggc aagctgggac ctgaaagaca
     1501 aaattgcctt attgcgcaag atggaccaga tggccagagc ctacgacccg cgcataagca
     1561 atgtttcggt cagcctgggc gtggaagact acaccatcat tatcgccacc tcccgcggtg
     1621 acatcttcgc cgacaaacga ccgctggtca gactcaatat ttcctgcctg gccgaagacg
     1681 gcagcaagcg cgagaacggc agctcaggcg gcggtggacg attcgacttc caacagctcg
     1741 aagatgaagc atactggcaa gaacatgtac tgatcgcagc caaacaagcc atcgaactgc
     1801 tgaacgccga tccggccccg gccggagaaa tggacgtagt tctgggagcg ggctggccgg
     1861 gagtattgat tcatgaagcg gtgggtcacg gtctggaagg cgacttcaat cgcaaaggca
     1921 cttccgcctt cagcagcctc atggggcagt cggtagcctc caagctggtt accgtcatcg
     1981 atgacggcac cattgccgaa agacgcggat cgctcaatat ggatgacgag ggcaccccca
     2041 ccggacgcac cgtgttgatc gagaacggca aactgaccgg ctacctgcag gacagacaaa
     2101 acgcctcatt gatgaaagcc ggattgaccg gcaacggacg ccgacaagac taccgcttcg
     2161 caccgattcc gcgcatgacc aacacttata tggccggcgg cacctccagc cccgatgaaa
     2221 taatcgcctc cgtcaagcgc ggcatctacg ccactacctt tggtggcgga caagtcgata
     2281 taaccaatgg tgatttcact ttcagcgcta cctgtgccta cctgatcgaa gacggcaaac
     2341 taaccaggcc aatcaaaggc gccaccttaa tcggcaacgg tcccaaagcc ctgcaccagg
     2401 tagccatggt aggcaacgat gtcagcctcg acaagggcat cggcacctgc ggcaaagacg
     2461 gacagggtgt gcctgtcggc gtcggtatgc ccactatccg cctgaacggc atcaccgtag
     2521 gcggctccaa gagttagcac gaacgtagac tagactaaat tagaaaggta ccgtaatggc
     2581 cactgccaaa ttagaagaca tcgccgccgc catcgtgcat caagctcgca aactcggtgc
     2641 cgctgaatgc gatgtcacca tcaccacccg caatgccgtg gaaacctcgg tccgactggg
     2701 gcaagtagaa aagctggagg gggcccagat gcgcggcctg caatttcgcg ccctctgcgg
     2761 tgcgaaaggc accaaaacag cctccagttc cacctccgat tttggcgcta aggccctcaa
     2821 gcaactggtc aaggacacca tcgccacagc cgaagcctcg gaagaggatc cctatgccgg
     2881 cttgccggag cctggcgacc tggcccgcga gtaccccgac ctcggtctgt ttgatccggc
     2941 tgtggaaaag atccctgtag aaaccaaaat cagcctggcc aaagaggcgg aggaagtagc
     3001 aaggggagcc gatccgcgca tcaccaattc ggaaggagcc agcttctccg acagcgccgg
     3061 gcagatcgtt catgctaatt cccggggctt tctgggcagt tatcatggca gccatgtgag
     3121 cctttccgtc ggggtagtag ccagtcagga cgacgaaatg caggtaggcg gctggtgggc
     3181 ttctaatcgc tccttcgaca aactggaatc agccccggcc atcggtctga aggcggcaga
     3241 aagagccctc aggcaactgg gctctcgcaa agtcaagtcc caggaagtgc cggtggtttt
     3301 tgatcccctc atggcggcgc gcctgatcgc ccagttcatc ggagcagcag caggctccca
     3361 tatttacagg aaaagctcct tcctggtgga caaactgggg caggtggtgg cgagccaagg
     3421 cgtcaacata atcgacgacc cgctcatggc cggccgcctt ggttctcacc ctttcgactc
     3481 ggagggtcta ccggccagaa cccgtaccat catcgaagaa ggacgactgg tcacctattt
     3541 actctccagc tatgcagccc gccgcctcca cacccgcccc aacagcggta gcctcggcaa
     3601 cctctacctg caggccggca aggagagccc ggaagagatc atcggctcgg tcaaaaacgg
     3661 gctctatttg accagtgtct ccggccccgg cttcaaagtc attaccggcg actattccct
     3721 cggggccagc ggcatctgga tagaggacgg taagcttgcc tatcccgtct cgggcataac
     3781 cattgcctcc gacatgctca gcatgtttgc cggcattgaa gccatcggca acgatctggt
     3841 cttccgttcc agcacggcca gccctacaat taagataggc aaaatgactg tagcaggaca
     3901 gtagatgttg agttccaaaa gaacagcaat gcagctcgcg ctgagaaagt tgacactgct
     3961 tacggtgacg acctctctgg ctgcatcaat aaatctgctt caagggcaga cgccagcatg
     4021 ggcagccaaa aaggaagcgg cctcagtgga ggttgccgta gtagcgcgca acttgatcgg
     4081 cgccaagcgt tttcaggaag ccttgcaatt gaccggcgac aaaataaagg tgatggaaaa
     4141 cgacaggtca tcgcatatca gccccggtaa ctggcgcctc gacggagtcg ggcacgcgct
     4201 cgccttccag atgctgaccc agcctcaact catggaaatc tacctgggag acatctacaa
     4261 tctgcacgga ctttgccttt accagctcaa gagaattccc gagtcgctca aatatgtgca
     4321 acttgccctc gaccacaatc cctatcaggc ccaggccctg caaaacaagg gcgtcatact
     4381 ggcaaaaatg ggcaagcacg acgaagccat caagtgcttc gatcaattgc tgcgccagaa
     4441 cgagcgcttt gtcggtgctt atctgaatag agcagaaatt tacaagatca agaaactcta
     4501 cagtctcgag caaaaagacc tggacaatta tcacaaattc aagattctga atcagaaaca
     4561 ggaattgaat gaaaccatct tgagccgctt cgaatatgcc tatcatatta tcaaaggcaa
     4621 accgcgcagc cccgagcagc tcaccgccct cggtatcatt cactgggaaa atctcagact
     4681 gaaggaaagc gaagaaagct tcaagcaagc cattcagctc aatcctaaat acgcaccggc
     4741 ccacctgggt tacagcattt ttctggaatg gatgaaacgg cacaaggatg ccatcaggga
     4801 aagcagtctg gccctttccc tcgacaagaa tattcccatt gcttacatca cccgagcccg
     4861 ggcccgcatt gccgagaatc aactgccagg ggccattgac gattacaata cccttctgaa
     4921 cacggaaaag tcagacaccg ggctgactat cagagccctg ggcaaccgcg ccgcctgcta
     4981 tgccaaattg gctcgctaca aagaagccat agaagacgtc aaccatgccc tttcgctccg
     5041 accggcagca gcaaccaagg ctgagcttct ggccaaccgc ggtctttgct atgaaaaact
     5101 gggcgataaa caacaggctc tcaaagacta cgactcagcc ctggcagcta atccaggcga
     5161 aagaagagct tacgaaaatc gcggcaaaat tatgctttcc atgggtcagt acgaacaagc
     5221 catggccgac ctgaacacct caggaagcgc cgctgcagaa gccagtccct cccttcccac
     5281 cggcaaagaa ttgtccagca tggtggctca ctatgacaag cttctcaaac tctttccaaa
     5341 ctccaccgag agcctctaca accgtggact gctgcagctt accctgggca aaccggcact
     5401 ggccgcccag gatcttcaga aagtggtaag cctcagcaag caagccaaca acacctccga
     5461 ttatgccgtt tgttttgcca atattgctct ccggctggat ggtcaaaagg aaaaagccaa
     5521 agaattgatc aatcaatatc ggctgctcaa gcggcaagat cttgcctcgc cggtagtaga
     5581 ttacttcatc aagggcaaga cctcggtgct caccaccgcc acctccaata gtggcaatcg
     5641 ggccgggcaa acccgctcgc ttactctgct agggctcgat gcctacgcca gcggcgacaa
     5701 agtacaggcg cgcgaatatc taagcaaggt cagacacgaa ggcgacactt ccaccgatga
     5761 atttactctt gcgctttctt actggaacaa actctagaga gccctgaaga ttcactaaag
     5821 caagaccgga cgcacccagg ccttcagatc gggcaattca tgcacgcgct tggaagccgt
     5881 caatagccgt tcgtaaatat cgagaatgtg aggcgcatca ggatgggtag ggaaatccct
     5941 ggcctgatcg taggcatgct caagcactcg ctcggccatc tcctcatcac cggcttgcaa
     6001 caagagttcg gcaagcctgg ccagagaggt gaggttatag ccggccggcg gcttaaaggc
     6061 ctgctgcatg gaagccagaa cagattcgga cagcctgacg gcatcctcac gacgaccggt
     6121 ttccaggcag attatcgctt gaaaaatgcg aatatgtata tcaagagaat ggaagctctc
     6181 ttccatcacc gctagcggtc gtccgctgcc gtgtacaaaa gcaagagccc gttccaattt
     6241 gctttctgcg gccgtgaaac tgcctcgcaa aacatctatt tcagccagat tgacgagcgg
     6301 aaaagcctga ccaagcgcct cgtttccctt cagtttggac caaatttcca gggctttgag
     6361 agaattggct tccgcttctt ccaattcacc ctggcgcaaa tgagcatagg ccagattatt
     6421 gacgtagatg gccgcaagag cattcttagc tattttcttc tcccggccga ggcgctccat
     6481 caactcacca tagatcaatt ccgattcggc aaactgccca ctggctagtt tcacaaccgc
     6541 cagattggaa actgccgtca tatagctcca gtctttgcga agccccaggc gacgcaccat
     6601 attcaaaccg cggttgagaa ccttctcggc atcatgcaat tgaccacggg agagcaatct
     6661 cactgagtaa aaaatcaccg gagagagaat gagataaccg gagaaaagat aaagacacac
     6721 cagaaagaga gtaagagaaa ccaccgagga gagccattcg ccggcataga gcgaaccgat
     6781 aagggcggca gaaacgcagg caaaacaaag agccatatag agccaccaga atacagccag
     6841 gaacacttgc cggccgccgc ccttgcgttc atagggcact atttgcttga gttttccatc
     6901 ctcgctcaag aaagactact cccctcctcc agacaagcct gaagcctcaa aatcaactcc
     6961 ggcattatta tgcccagatt catgggcagt cacgatactc tctccaaaga gatatttgct
     7021 atataattta gtattacacc gaaaaatgaa tcgagaacat ggctgtttgc acatagatac
     7081 cggcaaaggg aaaccaggaa gcagagattg cacccgcgcc agagcttcca ggttgctttt
     7141 catggcaacg gtcgaagctc aagacgcggg tgcaggaact gtggtggtta gccgcgcatg
     7201 ccgttgaaga agaactcaaa ggcaaggatc aggatgaacc agaacaagag cgaaatgccg
     7261 atacctgcca gagaagcaag caagaagacg accacgctgt tacccagggc atagaccagg
     7321 gccgaagcca gattgcgcat gccattggaa ctgattcggc tgatttcacc ggcgaacatg
     7381 ccctgcaagt accggccggc atagctgcca accagagaga agagcacgag aagaacgagt
     7441 tgcatcagat tcataattga ctcctgagga gtagttacgc ctgtttgagc aggcaagatt
     7501 cgactttcca gacccaccgc caggcagccg ctaagttagc ttcgacttca ggctcaatct
     7561 ccacgactgc aagacgagcc ttagctgaaa tcaagcccaa accgcagcgc caagccaggc
     7621 agagaattag ccttccaacc catactgata ctgccgactg ctggtgaaat gctgccggac
     7681 gagaaagacg agtgtgagag gtaaatttct aaggctgcaa agaaaatccg gtaggtgaaa
     7741 agaaagacac tctgttttag tgaatttgag ctctcttctt ccagtaggga aagcaggagt
     7801 cggcacatgc caagtggcat tttccacccg gcttgctttg ctgtccgccc cgaattccct
     7861 tttcagaatc cgcaatcggg caaagggcgg gggctgaact gcattgaggc agaaccacgg
     7921 acaggctctt ggctagcctg cgcgcgcgaa ccttcgcctg gaaatttagt taaggagcgg
     7981 tctgaaccga cccaccaggc tccctgtaca acagctactg caaaggctga aagagaccga
     8041 actcttttct ggcccattcc gt
//
LOCUS       DAOQNZ010000006         7973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3366_length_7973_cov_3.957565, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000006 DAOQNZ010000000
VERSION     DAOQNZ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3366_length_7973_cov_3.957565"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="PKW73_00215"
     CDS             <1..1774
                     /locus_tag="PKW73_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71729.1"
                     /translation="DTGLPLGRVLVLSGLISENDLSNLILCQSLLREDLVQLEQVRNA
                     FLRVRGSGRRLEDALLELGWERSLEKQLSPLGELLVSSAVITESQLDTYLRQQERVRL
                     PLGRMLVSAGVMTDAFLSTALNVQMMIRQKRLSREEAVDVLVEARKRQLAQSNVPRAK
                     NFYEQPVLNVPKLGELLVLSGLLSERKLLEALELSIIGRKSIGEILLEKRYLTKTQLD
                     NILLLQSSLAEGIIKLPQLKSVLRRLEDGFSLSDAIGQATEQVAVDTSEGRVLTFFEF
                     LKSLDHTSGTNIDQAFEMAKKNQRLVKQALLISGMLDEPTIELMEQCYALYEDRRYSF
                     DAASTLFEYSRRRGISVSDALEELRWLKKPPCIAVEPTEKFIKATDSSLLNMKEMAEQ
                     LIALRDLVNARALYEQLLNALSVYKDGRYRYCLERIAYIFFEQKDYLKAENYQRELTR
                     LSLECYGENSIPHAQALNNLGKTLYFMGRIDDALHETLAYIEVCANLLGADHPDVACG
                     WQNAAMLYFQLGDIAESLRCYKEAHKICRESLGPSNPVTLNLEAKVDGLRERVVTSRR
                     SLEGLITGSWRTIDLGASLNSMSE"
     gene            complement(1817..1990)
                     /locus_tag="PKW73_00220"
     CDS             complement(1817..1990)
                     /locus_tag="PKW73_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71730.1"
                     /translation="MEEDGTIVLKLIASGPDGLIGHGTLVYGREHEQYQEILQHVGPL
                     KPGENKPVAPWPD"
     gene            2109..2504
                     /locus_tag="PKW73_00225"
     CDS             2109..2504
                     /locus_tag="PKW73_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA71731.1"
                     /translation="MLATARIHHVAIICSDYERSKKFYHELLGFEIISEHYRAERQSY
                     KLDLQVGACQIELFSFPDPPGRVTGPEACGLRHLAFEVDDVAEVLRFLRDNGVEAEDI
                     RVDEYTGKRFTFFRDPDNLPLEIYESRAL"
     gene            complement(2510..4591)
                     /locus_tag="PKW73_00230"
     CDS             complement(2510..4591)
                     /locus_tag="PKW73_00230"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA71732.1"
                     /translation="MGGAYQVKSLVASGGMGTVYLVEHLQLKRRLALKTLKKDRINQS
                     AWNSFVLEAKVLARLEHPNLVKVYDLGIINNRLPYYVMDYVAGGTLATQIKESGPLSL
                     SKALSIFEQVAAGMAYAEDLVLVHRDIKPSNIMLVETSGKSPQVKIVDFGLAMQENGA
                     LSAGERERICGSPPYMSPEQTRNEHLDIRSDIYSFGCTLFEALTGSPPFIGESILIVL
                     SKHQLEAAPTLKEATLGGDFPAVMEGALAKMLAKDPAMRFQSFHEIAELLSKMRVLQE
                     ENRLSLVELNTRSDSDQAPALERKARNGSEKQLRKSEPAPSPLRIAAAAAAVALLAVG
                     AAAAMALKFMGQTGNNPPSNLLAFTGSGKLQEGAVLPESVEESQFIKEQQKSQDPIQV
                     LNAYYTQQEPFSLVQLEGKTWRRFNSPRIFNLGAFTAVNLSTVSGRVLLPAGKNYAGP
                     GTIIFDPRSLLIYSPSKLLASHPELLKNWKPDAVWGLDLANNRALSNETLGGFTPLTG
                     LKYLDISYTRAGDSAVEELNKFTALEGLAATHSELTGDGLVKFKGLLNLKTLKVGPIF
                     QLSRLLEKLSQSRQITYLEIADEPVSQAELQQIAAITSLRHLCLNGALSQSKYLPSLR
                     TLEHLETVELSKMKTYKYLVEDLKTLPVLKRLIIHESDLSTDECAALQKELPNLKIEL
                     APRVEQPEPKS"
     gene            complement(4688..6814)
                     /locus_tag="PKW73_00235"
     CDS             complement(4688..6814)
                     /locus_tag="PKW73_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA71733.1"
                     /translation="MNELSEKYLPTGTVIGGSYRLLKCLGEGGMGAIFEVEHVIMGKH
                     FALKLLTKSNIAETDWLRFKQEAQTLSKLQHANIVQVSDLGIHQAGEKKFPFYVMELL
                     QGESLKQRLKVCGRMGAREAVAIFESVAKALALVHDKGIIHRDIKPDNIMLVKGDSGI
                     TPKLVDFGILKLKDGGGVALTLQGEVFGSPLYMSPEQCMGQPLDPRSDIYALGASLFE
                     ALTGVPPLSGENAVETFKLKTSTRAPRLGDKVSLITFDDELEDLVADMLEMDANHRPP
                     SAAHVVGRLQAIRLMQEMGLPQVPGRKPEQPTGISTEPARRQTKPAPEAEEPARRQAK
                     PAPEAEEPAIEQSLTKKLFLPAVCLVALAALLCALALWQYTAPKSDSAGTKNSQMKDA
                     NFVGLPQIEPDNQEGSKPREESNKDWSQLEKIPVICELNSAAGQKQFHLPQTISIGSL
                     RLLKNGRATDSIDVRGELRFPADAIVSYTPNNLVLEHPELMDKCKDGVLKELVLSNEE
                     LVTNDFLKHLTHLKSLATVKLNNTMITDDGLKYLEQLPALEKLEIGTPGVRDKSLSQA
                     GFLLGLRHLDLKSQPTISLTLKALAKSSKLQKLVATYCNLSREDWQDLARIKPLRELN
                     LGSANLDDKSLEELISLPNLQELEIKDCPVTTGSAKVLKRFPKLRFLVIDSENWTKQE
                     FQDFAKTVAPCRIAKDKRDFTNFNLQ"
     gene            7027..>7973
                     /locus_tag="PKW73_00240"
     CDS             7027..>7973
                     /locus_tag="PKW73_00240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNA71734.1"
                     /translation="MSTSAEPEKSKQAIDLKTKTILDSIFGLVLFTTITFVLAPPPSA
                     VFQVTDLSEFYCGAALFQAGHSADIYDVAKFFPFQVQTFPALGTRAVPLYVAPFGLPF
                     LLPLLIFPTAVAYLCTKILLIGCFLLGLFLLVRVFKLSSRWFMWLSAILPFSGPVWEA
                     LRIEQVSPMLFLSMAAHLYFLERKRPVLSALCLLPFLVKPHLAMTMVAFHLGGCRFRF
                     CLAFLGFSLGFLISSYFYCGAEAYKNYFALLKFSMQDLRWMNPEATPTLRGQLLRLTV
                     LPTAAVNSITSAIFALLQLFVVGFGFKLRKRSAARAAALV"
BASE COUNT         1573 a         2042 c         2179 g         2179 t
ORIGIN      
        1 cgataccggc ttgcccctgg gtcgggtgct ggttctgtct gggcttatca gcgaaaatga
       61 tctgagcaat ttgattttat gccagagcct gctgcgggaa gacctggtgc agcttgaaca
      121 ggtgcgcaat gctttcttgc gggtgcgcgg ttccggtcgg cgcctggaag atgcccttct
      181 ggaactgggc tgggaacggt ctctggaaaa gcagctctcg cctctaggag agctgcttgt
      241 ctcttctgcg gttattaccg aatcgcaact ggatacctac ttgcgccagc aagagcgggt
      301 gcgcttgccc ctggggcgta tgctggtttc tgccggagtt atgaccgatg cctttttaag
      361 cacggctctt aatgtgcaga tgatgataag acaaaagcgt ctcagccggg aagaggcggt
      421 agatgttctg gtggaggcgc gcaaaaggca gttggcgcag tctaacgtac cacgcgccaa
      481 gaatttctat gaacagccgg ttttgaacgt gcccaagctc ggtgaactgc tggttctgtc
      541 agggctgctc agcgagcgca agctgttgga ggcgctcgaa ctgagtatta tcggtcgcaa
      601 atccatcggc gagattcttc tggaaaagcg ctatctaacc aagacccaac tggacaatat
      661 tttgcttctg caatcaagtc tggccgaggg cattatcaag ttgccgcagc tcaagtcggt
      721 gctgaggcgt cttgaggacg gcttttccct ctctgatgcc attggtcagg ccaccgagca
      781 agttgccgtc gatacgagtg aaggccgggt tcttactttc tttgagtttc tcaagtcgct
      841 cgatcatacc agcggcacca atatcgacca ggcttttgaa atggccaaga agaatcagcg
      901 tctggtcaag caggcactct tgatctccgg tatgctcgat gagcctacca tcgagctgat
      961 ggagcagtgt tatgctcttt acgaagaccg gcgctactcc tttgatgccg cctcgacctt
     1021 gtttgaatat tcgagacggc gcggcatatc ggtatccgat gccctggaag aattgcgctg
     1081 gctgaagaag ccgccctgca tcgcggtgga acctacggag aagttcataa aagccaccga
     1141 ctcttctctg cttaacatga aagagatggc ggagcaattg attgccctga gagacctggt
     1201 caatgccaga gctttgtatg agcagctttt gaacgccctg tccgtttata aagacggacg
     1261 ctatcgctat tgtctggaga gaattgcata tatcttcttt gagcagaaag attatctcaa
     1321 ggcggaaaac taccagcggg aattgactcg cctcagtctg gagtgttacg gagaaaatag
     1381 tattccccat gcccaggctt tgaataatct gggtaagacc ttgtatttca tgggacgcat
     1441 tgatgatgct ctgcatgaaa ccctggctta catagaggtt tgcgctaatt tgctgggcgc
     1501 cgatcatccc gatgtggctt gcggctggca gaatgccgcc atgctctatt ttcagctcgg
     1561 tgacattgcc gagtcactgc gctgctacaa ggaagctcac aagatttgcc gtgagagcct
     1621 tggtcccagt aatccggtca ccttgaatct ggaagcgaag gtggacgggc tgcgggagcg
     1681 agtggtgact tccaggcgtt cgctggaagg actaattacc ggcagttggc gtaccattga
     1741 cctgggtgct tccctcaatt ccatgagcga atagggctta tgcctcagtg ctattctgtg
     1801 ttgcttgcgg cctttgctag tcaggccagg gagcaaccgg tttgttttcc cccggcttga
     1861 ggggacccac gtgttgcagt atttcctggt attgctcgtg ctcccgtccg taaacgaggg
     1921 taccgtgacc gatcagtcca tccggaccag aggctatcag cttgagcacg atagtgccgt
     1981 cctcttccat ccagcaaact ccaatggatt caggttggtt ttgcttcatt tgtttcgccg
     2041 gtcccatcgg taatttccct ggtttcttgt agactaaaag gcaccaggac aggtagaaaa
     2101 ggaattttat gctcgccacg gccagaatac atcatgttgc cataatttgt tcagactacg
     2161 aacgttccaa gaagttctat catgagctgc ttggctttga gattatttcc gagcattata
     2221 gagcggagcg gcagtcttac aagctggatt tgcaagtggg tgcttgtcaa attgagctat
     2281 tctccttccc cgatcctccc ggtcgtgtta ccggacctga ggcttgcggc ttgagacacc
     2341 tggccttcga agttgatgat gtggcagagg tcttgcgctt cttgcgggac aatggtgtag
     2401 aagctgaaga tattcgagtg gatgagtata ccggtaagcg ttttactttc tttcgcgacc
     2461 cggacaatct gcccctggaa atctatgaaa gtcgggcact atagatgatt tagctctttg
     2521 gctccggttg ctccacccgc ggtgccaatt cgatttttaa attgggcagc tctttttgca
     2581 gggccgcgca ttcatctgtg ctcaggtcgc tttcatgaat gatcaatcgc ttcaggaccg
     2641 gaagcgtttt caaatcttca accaggtact tgtaagtttt cattttggaa agctccactg
     2701 tctcaaggtg ttcgagcgtt ctgaggctgg gcaggtactt ggattgggag agcgcgccgt
     2761 tcaagcagag gtgccgcaat gaggtgattg ccgcgatttg ctgcaattca gcctggctta
     2821 ccggttcgtc ggcgatttcc agataagtga tttggcggga ttggctgagc ttttccagca
     2881 gccttgaaag ctggaagata ggtccgactt tcagtgtttt caaattcaaa agacctttga
     2941 atttcaccag tccatcgccg gtcagttcgc tgtgagtggc agccagacct tccagtgctg
     3001 taaatttgtt gagctcttct acggcgctgt caccggcgcg ggtgtaggag atatcgaggt
     3061 acttgagccc ggtcaagggg gtgaacccac ccagggtttc gtttgagaga gcacggttgt
     3121 tggccaggtc gagaccccag acggcatcag gtttccagtt cttcagtagt tccggatggg
     3181 atgccagcag cttgctggga gaataaatca aaagagagcg cgggtcaaaa atgatggtgc
     3241 cgggaccggc atagttcttg cctgccggta gcagcaccct acctgaaacg gtagagaggt
     3301 tgacggcagt aaaggcgccc agattgaaaa tgcgagggct gttaaagcgg cgccaggtct
     3361 taccctccag ttgaaccagg ctgaagggtt cttgttgcgt gtagtatgca ttaagtacct
     3421 gaatcgggtc ctgggacttc tgttgctcct ttatgaattg gctttcttcg acgctttcgg
     3481 gcaggacggc accttcttgc aacttgccac tgccggtgaa ggcaagcagg ttgcttggtg
     3541 gattgttacc tgtttgaccc ataaacttga gagccatggc agcggcagcg cctacagcca
     3601 ggagcgccac ggctgctgct gctgctgcaa tgcgaagggg ggagggagcc ggttcggact
     3661 tcctcaattg tttttctgag ccgtttcttg ctttgcgctc cagggccggc gcctggtcgc
     3721 tgtcgcttct ggtattcaac tccaccagag agagacgatt ttcctcctgc agtacccgca
     3781 tcttagatag cagttctgct atttcatgaa acgattgaaa gcgcatggcc ggatctttgg
     3841 ccagcatctt ggccagggct ccttccatta ccgctggaaa gtccccgccc agagtggctt
     3901 ctttcagtgt cggggcggct tccagttgat gcttactgag tacgatcaag atggattcac
     3961 cgatgaatgg tggagaacct gtgagagcct caaatagcgt gcagccgaag gagtagatgt
     4021 ccgagcgaat gtccaggtgc tcatttctgg tttgctcagg gctcatatag ggtgggctac
     4081 cgcagattct ttctctctct ccggccgaca gggcgccgtt ctcctgcatg gccagtccga
     4141 aatccactat tttgacttgc gggcttttgc cgcttgtctc caccagcatg atgttgcttg
     4201 gcttgatgtc acggtgcacc agtaccaggt cttccgcgta ggccattccg gccgccactt
     4261 gctcgaagat ggatagggct ttgctcaggc tcaagggtcc gctttccttg atctgggtgg
     4321 ccagggtgcc gcctgctacg tagtccatta cataataggg caggcgattg ttgatgatgc
     4381 ccagatcgta caccttgacc aggtttgggt gctcaaggcg agccagcact ttcgcttcca
     4441 gaacaaagct gttccaggcg gattggttga tgcggtcttt cttgagggtt ttcagtgcca
     4501 ggcgacgctt tagctgcagg tgttcgacca ggtagacggt gcccatgccg ccggatgcca
     4561 ccagagactt tacctggtag gcaccaccca cgatatcgtc agcgcttaag cccgcttcgc
     4621 ggttggcctg gttgttcagg cgtttatcgc tgcctttgcc ctgcttgcct tcgattatcc
     4681 atactcctta ctgtagattg aaattggtga aatcgcgttt gtcctttgct attcggcagg
     4741 gggcaacggt ctttgcaaaa tcttggaatt cttgcttcgt ccagttttca ctatctatga
     4801 ccagaaatcg caatttgggg aagcgcttca gcactttggc actgcctgtg gtgaccgggc
     4861 agtctttaat ttccagctct tgcaaattcg gcagagagat cagctcttcc agagacttgt
     4921 cgtccaggtt ggcagagcct agattgagtt ctctcagggg cttgattcgg gcaaggtcct
     4981 gccagtcttc ccgggagaga ttacagtaag tcgccacgag cttttgcagt ttgctgcttt
     5041 ttgcaagtgc ttttaaagta agagaaatgg tcggctgact cttcagatcc aggtgcctga
     5101 gacccaggag aaaacctgct tgactgaggc ttttgtccct tactcccggg gtaccgattt
     5161 cgagcttctc cagagccggc aactgttcca gatatttgag tccgtcgtca gtaatcattg
     5221 tattgttcag cttgaccgtg gccaggcttt tcaaatgggt cagatgtttt aaaaagtcgt
     5281 ttgttaccag ttcctcgttg ctcagtacca gttcttttag cactccgtcc ttgcatttat
     5341 ccataagttc cggatgttcc agaaccagat tgttaggagt gtaggagact attgcatcag
     5401 ccgggaagcg cagttcccct cgtacatcta tggagtcggt ggctcgtccg ttttttaaaa
     5461 ggcgcaggct gcctatggaa atagtctggg gcaggtgaaa ctgcttttga ccggcggctg
     5521 aatttagttc gcaaatgacc gggatttttt ccagttgcga ccagtccttg ttgctttctt
     5581 cccggggctt gctgccttcc tggttgtccg gctcaatttg cggcagcccc acaaagtttg
     5641 cgtccttcat ttggctgttt tttgtgcccg cgctatcgga tttgggtgcg gtgtattgcc
     5701 agagagcaag ggcgcaaagt aatgccgcca gagccaccag gcacacggcc ggtagaaaca
     5761 acttcttggt cagggactgt tcgattgccg gttcttcagc ttccggtgcg ggctttgctt
     5821 gtcgacgtgc cggctcttca gcttccggtg cgggcttagt ttgtcgacgt gccggctccg
     5881 tacttatgcc ggttggttgc tccggcttcc tgccgggtac ttgcggcaga cccatctcct
     5941 gcatgagcct tatggcttgt aagcgcccca ccacatgagc ggcagagggc ggccgatgat
     6001 tggcatccat ttccagcata tcggccacca gatcttccaa ctcgtcatca aaggtgatga
     6061 gggagacctt gtctcccaac cgtggggcgc gggtgctggt tttcagtttg aaagtctcca
     6121 cggcattttc cccggagagt ggtggcactc ccgtcagggc ctcgaagagc gaggctccca
     6181 gggcatagat atcgctgcgc ggatcgaggg gctgacccat gcattgctcc ggactcatgt
     6241 agagggggct gccgaaaact tcgccctgga gagtcagagc gacgccgcca ccgtctttca
     6301 gctttaagat gccgaaatct accagtttgg gcgttatacc tgagtcacct ttgacaagca
     6361 ttatgttgtc cggctttata tcgcggtgga tgattccctt atcgtgtacc agcgccagtg
     6421 ctttggctac actctcaaaa attgctaccg cttccctggc gcccatgcgt ccacagactt
     6481 tcaatctctg cttgagagac tctccctgca gaagctccat tacgtaaaag ggaaacttct
     6541 tctccccggc ctgatggatg ccaagatcgc ttacctgcac aatatttgca tgttgtagct
     6601 tggaaagggt ctgggcttcc tgcttgaagc gcaaccagtc ggtttctgct atgttgcttt
     6661 tggtcagcag tttgagcgca aaatgcttgc ccataattac atgctcgact tcgaagatgg
     6721 ctcccatgcc gccctctccc aggcatttga gcaagcggta ggaaccgccg ataacggtac
     6781 cagtgggtag atatttttcg ctgagttcat tcacaggcgt taggagcctt caatccgacc
     6841 gctgatttac taaagtgaga ttccgtgatt aagggccacc ccacactgac tttatgccca
     6901 gagccgggcc tgaatgctta agtcagggcg gggcgggcca ttaattagct gcttttttac
     6961 accggagcgc aaatctaact ggctttgctt tatgatctgg cagcgtctct tgggggagtc
     7021 cttttaatgt ccacctcggc tgagccggaa aagtcgaaac aggcaataga tctaaaaacg
     7081 aaaacaatac tggatagtat ttttggtctg gtgcttttta ccaccatcac ttttgtgctg
     7141 gcgccgccgc ccagtgccgt atttcaagta accgatctgt ccgaattcta ttgcggtgcc
     7201 gctctctttc aagccggtca cagcgctgac atttacgatg tagctaaatt ctttcccttt
     7261 caggtgcaga cttttccggc tcttggcacc cgtgccgtgc ccctttatgt tgcgcccttc
     7321 ggcttgccct tccttttgcc gctgcttatc ttccctactg cggttgctta cctgtgcacc
     7381 aagatcttgc tgattggctg ctttctcctt ggtctcttcc tcttggtgcg ggtctttaag
     7441 cttagtagtc gctggtttat gtggctttcc gccattttgc ccttcagtgg tccggtctgg
     7501 gaggcgctgc gcatcgagca ggtctctccc atgctctttc tgagcatggc cgctcatctc
     7561 tactttcttg aaagaaaaag accggtgctc tcggcacttt gcctgttgcc ctttctggtc
     7621 aagccccatc tggctatgac gatggtggcc tttcacctgg gcggttgtcg ctttcgtttt
     7681 tgcctggcct ttctgggctt cagtctcggc tttctaatct cttcctattt ctactgcggt
     7741 gcagaagcct acaaaaatta ttttgccctg ctgaaattct ccatgcagga cttgcgctgg
     7801 atgaatcccg aggctacgcc aactttgcgg gggcaacttt tgcgtctgac ggtattgccg
     7861 acagccgcgg tcaattctat taccagcgct atttttgcgc tgctgcaact gtttgtggtg
     7921 ggcttcggtt tcaagcttcg caaaaggtcg gcggcacgtg ccgctgccct ggt
//
LOCUS       DAOQNZ010000007         7756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3525_length_7756_cov_3.615764, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000007 DAOQNZ010000000
VERSION     DAOQNZ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3525_length_7756_cov_3.615764"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /gene="yidC"
                     /locus_tag="PKW73_00245"
     CDS             complement(<1..634)
                     /gene="yidC"
                     /locus_tag="PKW73_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNA71735.1"
                     /translation="MDFITYQLMIPFMQMVVKETHSYGLAIILLTLAVRALVWPLVSK
                     STKNMQRMGKLSPMLKELQERYKDDQEMLQRKMMEFWTVNKVNPMSGCWPMLVQLPIL
                     FALFGTFNGPPFSDHAIDVPVKVVEAKDISKRNHKEVSGANCPYVSHDGLVAKLIVFP
                     GETTSVEGDKVDFATRAVEGKLPADFKVKWQVQQGNAPAKAEDAVIDQEGH"
     gene            complement(655..966)
                     /gene="yidD"
                     /locus_tag="PKW73_00250"
     CDS             complement(655..966)
                     /gene="yidD"
                     /locus_tag="PKW73_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNA71736.1"
                     /translation="MKYLTNTAIFKQDNKKIGLMSRVALVLIWLFQQTRILRQPSCRF
                     YPSCSQYTADSMKRFGFLKGLVLGTVRLAKCHPWHDGGVDDVPESFEPLKMLSGHPGT
                     R"
     gene            complement(1062..1592)
                     /gene="rnpA"
                     /locus_tag="PKW73_00255"
     CDS             complement(1062..1592)
                     /gene="rnpA"
                     /locus_tag="PKW73_00255"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNA71737.1"
                     /translation="MLPTAERARKSSVFTRAYTARRSVSSGCLTLYVLHRFTPRRLAK
                     AKGGAQAVGAAKALNSVSGASAGQASQPEQVRAAAAGITISGQIRLLVGFSIAKKVLK
                     SACKRNRCKRRAREAYRAVRSEAALVKELSEGLSNWYALVFVISPDALKVTFEELKAA
                     VRDCLTKANKKFGRPN"
     gene            complement(1602..1736)
                     /gene="rpmH"
                     /locus_tag="PKW73_00260"
     CDS             complement(1602..1736)
                     /gene="rpmH"
                     /locus_tag="PKW73_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNA71738.1"
                     /translation="MKRTLRGSISKAKKGSGFLKRMSTKSGQKVIKARRAKGRYRLSV
                     "
     gene            2111..3643
                     /gene="dnaA"
                     /locus_tag="PKW73_00265"
     CDS             2111..3643
                     /gene="dnaA"
                     /locus_tag="PKW73_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNA71739.1"
                     /translation="MTNSNNDDVRLLKSNKDIWSEARIILEKKLSQPSFESWIRPAQL
                     IEFSQGQATIAVSNEFMRGMFTNNYVDSVCDAISQVTGENVSIRVVVDASVKSETYTA
                     TIASISDEPNNTAQHDSQANHYLGNSNSGHGHNGHHLLGNQSSPPWATNNQQPVSTSA
                     RLPDSVLTARSNLNAKYLFETFVVGAHNRFSHSAALAVAQRPGQAYNPLFIYGGVGLG
                     KTHIMHAIGHEILRHTPNASVRYISCEKFTNELINSIRDDRMSDFRKRYRQVDVLLVD
                     DIQFLQGKESTQEEFFHTFNALRDSGRQIVLSSDRPPKAISHLEERLRSRFEWGLIAD
                     IQAPDLETRVAILRKKCEAENMRIDDEALEYIATVFTSNIRELEGALIRANAYCSLTG
                     EALSITSLAGMLQPTVPGNKPKVTITADRIIQTVAAHFRVEPSELRSAKRSQDLALPR
                     HVAMYLAHELIKMSFPRIGEAFGNRKHTSALYAHSRIKEALPKDPNLAQAVKQIQGQL
                     AD"
     gene            4140..5327
                     /gene="dnaN"
                     /locus_tag="PKW73_00270"
     CDS             4140..5327
                     /gene="dnaN"
                     /locus_tag="PKW73_00270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNA71740.1"
                     /translation="MHFAVQKDVLVKALKDVTSALATRVVQPILSNVLIESVDETAVK
                     FHGTDLDLTIETKASAVVYTPGAITLPGKKLLEIVSKLPSDLVSFQINKDTFETTVTC
                     KRSKFSITGLPADDFPKTTETRSKDGVLMPSDVLRKSIVQTAFAAASFDTSSILGGVF
                     LVIENGNFECTATDGSRLAHRFEKLNVAVPAASRTEGETETEGEAKSATATLEKPLSL
                     KAIIPAKSCTEIVKVLDSQDGKDVRLAVVNGQITFETETHFLSTRLISGEYPAYQTLF
                     PKDYSHLVSFKREEVMQAIERVAVMSDDRTHLVKLHFEPEMLQISANTPDVGRAQEEV
                     SIGLEGESIDVAVNVRYLTDVLQRLGVEDVKLEMTGALKPLIIKGIGDENYKYLLMPV
                     QAK"
     gene            5346..5774
                     /locus_tag="PKW73_00275"
     CDS             5346..5774
                     /locus_tag="PKW73_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71741.1"
                     /translation="MNLIQSSLAILLFLAGFLCPAALADTWSDLKSVSQAPSLIYCEG
                     GDSNQPTQLWLCFNRLYRANSVNAGTLKGKLNSATPAGKIYLLLLLRQTDPTEGSHLM
                     EKLLKNKEKVLYKKGCMLETMTVEKLCRHLAAGQELVKLR"
     gene            5827..6987
                     /locus_tag="PKW73_00280"
     CDS             5827..6987
                     /locus_tag="PKW73_00280"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNA71742.1"
                     /translation="MTQKQKPEPRAKSFVGTLAELCVIGQFVILAFLGGYVLAVYKNM
                     AELDAASQTAANTTLKAQRPASPGTVTPEAAKSGGDSSGEIVMPEPSPEKLSTSSISQ
                     EDLSDLDARRREHFARLIHLPPGRSAKRKGKGKPVLVEKRKLLGTPFYIATVDLTDPD
                     AFVGILLANGASRANCAGFSSGDEDFRSFVSRANAAVVMNGTFFSKDQEKRVMGNMVS
                     GGKTLKYSQWENYGTTVGITASNVLEMQTARLEGQPDWSKHWFSLTCGPRLVKNGVAQ
                     VDAKAEGFADSHVLSIGPRVAIGYPASKDKLYLVTFINGLSLEQEARLMKAIGCSEAM
                     NLDGGASRAFAVGKDVKVSPSRPLTNVLVVYDSAHPAPAALSQSWRKFEYSP"
BASE COUNT         1914 a         2041 c         1869 g         1932 t
ORIGIN      
        1 cgtgcccttc ctggtctata acggcatctt cagccttggc aggggcattg ccctgctgca
       61 cttgccactt cactttgaag tcggcaggca acttaccttc tacggcgcgg gtggcaaaat
      121 ctactttgtc accctcaacg ctggtagtct caccggggaa aactatcagc ttggccacca
      181 gcccatcgtg actgacataa gggcagttgg cgccggatac ttctttgtga ttacgcttgg
      241 agatgtcttt tgcttcaacc actttcacag gcacgtcgat agcatgatcg ctgaagggcg
      301 ggccgttgaa agtaccgaag agagcgaaaa gaatgggaag ctgaaccagc ataggccagc
      361 agccgctcat gggatttact ttattgacgg tccagaactc catcattttg cgctggagca
      421 tttcctggtc gtctttataa cgctcttgca gttccttgag catcggagac agtttgccca
      481 tgcgctgcat gttcttggtt gatttggaaa ccagaggcca gaccagagct ctcactgcca
      541 gagtgagcag gataatagcc agtccatagc tatgagtctc ttttacgacc atctgcataa
      601 aaggaatcat caactggtat gtaataaagt ccactctcag gttacctcag ataactacct
      661 tgtaccggga tggccactaa gcattttcag tggttcaaag ctttccggta cgtcatccac
      721 cccaccgtcg tgccatggat gacatttggc cagacgaact gtgcccagca ccaaaccctt
      781 cagaaagccg aagcgcttca tactgtcagc agtatattgt gaacaggatg gataaaacct
      841 gcatgatggc tgtctcaaaa tacgagtctg ctggaaaagc cagataagaa cgagcgccac
      901 ccttgacatt aagccaatct tcttattgtc ttgcttgaaa atagcggtat tggtcaggta
      961 cttcattact gaatgttgct cgggttcggc tctccagctc gatcactata gttatagtcg
     1021 aaagttggct ctctgtaacg atcgttggtg ctgccttcag gtcagttagg gcgaccaaac
     1081 ttcttgtttg cttttgtaag acagtctcgc actgctgcct tcaactcttc aaaggttacc
     1141 ttaagcgcat cgggactgat aacaaaaacg agagcgtacc agttgctgag accttccgaa
     1201 agttctttca caagagccgc ctcgcttctg acggctctgt aggcttctct cgccctgcgc
     1261 ttgcagcggt tacgcttgca ggcactctta agcactttct tagctattga aaagcctact
     1321 agaagcctga tttgtccgga tatggtgatg cctgccgcag cggccctgac ttgttcgggc
     1381 tggctggctt gcccggccga tgcccctgaa accgagttca aagcttttgc cgcaccaact
     1441 gcttgtgcgc cgccctttgc cttagccagt ctcctgggag taaagcggtg caatacatat
     1501 agagtcaaac aacctgaaga caccgaacgc ctggcggtgt aagcacgggt gaatacgctt
     1561 gacttgcggg cgcgttcagc agtgggaagc atcccaagct cttagacgga aaggcgataa
     1621 cggcctttag cgcgacgagc tttgattacc ttttgtccgg atttggtgga cattctttta
     1681 aggaaaccgc tacctttctt ggctttgctg attgagcctc tcagtgttct tttcatgacc
     1741 taactaactc ctgacctgaa ttcaagtaaa aattttttgg cgttctatct cgttctatcg
     1801 aaagaatagc tatgctatca tctattgcaa atacttcgag acgtctgccc tgaaggcgga
     1861 ctaaaaattt acgaataatt cctcacttag agaccattca ggtgagttag ggttcacttt
     1921 tggctcggca atcttgctgg gctatttgct tgtccctgat ttatcgaggc gacgggtcgg
     1981 gaattttgtt gggtctctta ctacaagtga aagatctgaa aatctgctct cgaaatgtta
     2041 tctggtaact tgaattcgcc ctgatcacaa ggctatctta gccaattacc acctaggacc
     2101 tgtgccgcta atgacaaact ccaataacga tgatgtacgc ctcctcaagt caaacaaaga
     2161 tatatggagc gaggcgcgaa tcatactgga gaagaagctc agccagccca gttttgagag
     2221 ttggatcaga ccggcgcagc taatagaatt cagccaggga caggctacca ttgccgtcag
     2281 caatgaattc atgcgcggca tgttcaccaa taactatgta gactcagtct gtgacgccat
     2341 ttcgcaggtt actggagaaa acgtctcaat cagagtagtg gtggacgctt ccgttaaatc
     2401 ggaaacctat actgccacca tagccagtat cagtgacgaa ccgaataata ccgcccagca
     2461 tgattctcaa gccaatcact atctaggaaa cagcaacagc ggacacggtc ataacggtca
     2521 ccatctgctc ggcaaccaat cgagcccgcc ctgggctacc aacaaccagc aaccggtgag
     2581 caccagtgct cgcctgcctg attcggtgct gacagcccgc agcaacctca atgccaaata
     2641 cctgtttgaa actttcgtag tcggtgctca caaccgtttc agccactcgg ccgccctggc
     2701 tgtggcccag cgacccggtc aggcctacaa ccccctcttt atatatggag gagtaggact
     2761 gggcaaaacc cacataatgc acgccatcgg tcatgagatt ctgcgccaca cccccaatgc
     2821 ctccgtgcgc tatataagct gcgagaaatt caccaacgag cttatcaact ccattcgcga
     2881 cgaccgcatg tccgacttcc gcaagcgtta ccgccaggta gacgtgctcc tggtggacga
     2941 cattcagttc ctgcaaggca aagagagcac ccaggaagag ttcttccaca cattcaatgc
     3001 cctgcgtgac tccggtcgcc agatagtgct gtcatccgac cgaccgccca aagccatttc
     3061 gcacctggaa gagcgcctgc gcagccgctt tgagtggggt ctcattgccg atattcaggc
     3121 accggacctg gaaacccgcg tggctatctt gcgcaagaag tgcgaagccg agaacatgcg
     3181 catcgacgat gaagcccttg aatacattgc cacagtattc acaagcaaca tcagagaact
     3241 ggaaggtgct ttgattcgtg ccaatgccta ttgcagtctt accggagaag ccttatcaat
     3301 cacttctctg gcaggcatgc tgcagcctac agtgcccggt aacaagccca aggtgacaat
     3361 caccgccgac cgcatcattc aaacggttgc agcccatttc agagttgaac cgagtgaatt
     3421 gcgctcagcc aaacgcagtc aagacctggc tttgccaaga cacgttgcca tgtatctggc
     3481 ccacgagcta atcaagatga gctttccaag aatcggagaa gcttttggca accgcaaaca
     3541 tacctctgct ctatatgcgc actctagaat caaagaggca ttgcccaaag accccaatct
     3601 agcgcaggcc gtgaagcaaa tccagggcca gttggcagat taactgcctg tagaaaactt
     3661 gtgcaaaact tgtttgttgc ttgtaagccc gttgtgtaaa tcccacccct tggcgtatat
     3721 acagcttttc cttatacaca tcccaccgac aagttttcta caggggatct tcaaacagag
     3781 tttgcctttc agggcttatc tacaaaaatt ggcttcccct cctacttcta tataactaaa
     3841 tttattgaac taacaagagt agaaatgcta aagatggctc aggttgccaa aaaccattca
     3901 aagggcgttg aaataattgc ccagtgacgg taacctttaa ggcacgggga gatagacaaa
     3961 ggaagatttc caatagagca agcggcgaca tggaaaacca ctcctttata tatacataga
     4021 atacccctgg tcccaaggcg cttcgttcaa agtcagtttg accggctaaa caggcaggta
     4081 aattgcggaa gaactatcga gcttgacggg atgcactctt tagatttcga ggttacaaaa
     4141 tgcactttgc agtccaaaaa gatgtcctgg tcaaagccct aaaagatgtg acaagcgccc
     4201 ttgccactcg tgttgttcaa ccgattctca gcaacgtgct gatcgagagt gtcgacgaga
     4261 ctgccgtcaa gtttcacggt acagatctcg atttgaccat tgaaaccaaa gcctcggctg
     4321 ttgtctacac acccggcgcc atcaccttgc ccggtaagaa gctgctcgaa atcgtatcca
     4381 agctacccag cgacctggtt tcctttcaaa tcaacaaaga taccttcgaa accacggtta
     4441 cctgcaaacg ctctaaattc agcatcaccg gtctgcccgc cgatgatttc cctaagacca
     4501 ctgaaactcg ttccaaagac ggcgtcttga tgccttctga tgttctgaga aagagcattg
     4561 tacaaaccgc ttttgccgcc gccagtttcg acacctccag catcctgggc ggtgtttttc
     4621 tcgtcattga gaacggcaat ttcgaatgca ccgccacaga cggctcccgc ctcgcccacc
     4681 gctttgaaaa actgaacgta gccgttccag ctgccagccg cactgaaggc gaaaccgaga
     4741 ccgagggtga agctaagtcg gccacagcta ccctggaaaa accgctcagc ctgaaagcca
     4801 tcattcccgc caagtcttgc acggaaattg ttaaagtgct cgactctcaa gatgggaaag
     4861 atgtgagact ggctgttgtg aatggtcaaa ttacatttga gaccgaaaca catttccttt
     4921 ccacccgttt gatcagcggc gaatacccgg cttatcaaac tctcttcccg aaagactaca
     4981 gccatctggt cagcttcaaa agagaagaag taatgcaggc catcgagcgt gttgccgtca
     5041 tgtccgacga ccgcacccac ctggtcaagc tgcatttcga acccgagatg ttgcagattt
     5101 cagccaacac ccccgatgtc ggtcgcgccc aggaagaagt ctccatcggt cttgaaggtg
     5161 agagcatcga tgtagccgtt aacgtgcgct accttaccga tgtgctgcaa agattgggtg
     5221 tggaagacgt caaactggaa atgacaggcg ctctcaagcc gctcatcatc aaaggtatcg
     5281 gcgacgagaa ctacaaatac ttgctcatgc ccgtgcaagc taagtaagcc gaaatcataa
     5341 acatagtgaa cctgattcaa tcaagcctgg ctattcttct ctttcttgca ggctttctat
     5401 gccctgctgc gttggctgat acctggtccg atctcaaatc tgtcagccag gcgcccagcc
     5461 ttatctactg tgaaggcggt gacagtaatc agccaaccca gctctggctt tgcttcaatc
     5521 gcctctacag ggctaattcc gtcaatgccg gtaccctcaa aggaaagctc aattctgcca
     5581 cccctgccgg caagatttac ttgttgctct tgctcaggca gaccgaccct accgaaggca
     5641 gccacctcat ggaaaagctc ctcaagaaca aggagaaggt cttgtacaaa aagggctgta
     5701 tgctggagac aatgaccgta gaaaagctgt gccgacacct ggctgccggt caggaacttg
     5761 tgaaactgcg ctaaaactgt aaaggcttgc ttctagcccg ggttgccgct tcgagaggtt
     5821 ttgcagatga cccaaaagca aaaacccgag ccacgtgcga agtcttttgt gggcactctt
     5881 gccgagcttt gcgtaatcgg ccagtttgtc attctggcct tcctgggcgg ttacgttctg
     5941 gcggtctata aaaacatggc tgagctggat gctgcatcac agaccgccgc caataccact
     6001 ctaaaggcgc agcggcctgc atcacctgga actgtcaccc cagaggcagc aaagtcaggc
     6061 ggagactcat ccggtgaaat agtaatgccg gagccgagcc cggagaaact atcgacaagt
     6121 tctatttctc aggaagacct cagcgatttg gatgctcgtc gacgcgagca ttttgccagg
     6181 ctgatacact tgccgcctgg tcgctcagcc aaacgaaaag gcaagggtaa gcctgttctg
     6241 gtagaaaaac gcaagttgct aggcacacct ttctacatag ctaccgtcga ccttaccgat
     6301 cctgatgcct tcgttggtat tcttctggcg aacggtgcca gccgggctaa ctgtgccggt
     6361 ttcagcagcg gcgatgaaga cttccgctct tttgtctctc gcgccaacgc ggccgttgtg
     6421 atgaacggta ctttcttcag caaagaccag gagaagcgag tcatgggcaa tatggtctcc
     6481 ggcggtaaaa cactaaagta cagtcagtgg gaaaactatg gcactaccgt gggcattact
     6541 gccagtaatg ttctggagat gcaaaccgct cgcctggaag gtcaaccgga ttggtctaag
     6601 cactggttct ccctcacttg cggaccaagg ttggtaaaaa acggtgtggc acaggttgat
     6661 gcgaaggcag agggtttcgc cgattcccat gtcttgtcca ttggaccgcg ggtggctatt
     6721 ggttaccctg cctctaaaga caaactctat ctggttactt tcataaatgg tctttccctt
     6781 gaacaggaag cccgacttat gaaagccatc ggctgcagcg aagccatgaa tttagacggc
     6841 ggggcatccc gtgcttttgc tgtgggtaag gacgtgaaag tatctccctc tcgccctctc
     6901 accaatgtgc ttgtcgtcta tgattcggct cacccggcgc cagcagcact ttctcagtcc
     6961 tggaggaagt ttgagtattc accttagtta ctagcgtgag caccaggtgc ccggcaatga
     7021 atgttcatct atgggcttgt gtcggaacac aaccgtgttg cccggtgcta ggctggaatt
     7081 gatgtgtgcg ttgaacttgg gcgaggttta ggcttgaatt cggaagaacc tagaggtagc
     7141 gagcctcctg cctggtacat agttttattc tggtgttatc ccatattgta tgtatttgtg
     7201 gttggtctgc cactgcgaat gctgccgccg gattcgaatc ttggagggat ttacggtcct
     7261 ttagttctgg ttggttttcc tgtgtccgta gcgtttttat atgatcggag cgcctggttt
     7321 agaaaagcat ttctggtagt tttgccggtg tggttattgc tctggggaat gtcttgcagc
     7381 ataactcagg gcgccttaag tgcggctatt tccaaggcgt tttcagccgt ggtcagtcaa
     7441 gcaaagaacc tacattgaaa gattgatgca attcaatcct tgaccagtcg gctttctgct
     7501 ttattgtcgg taccggctac aaatcgagtt gataaggcga ggtgccgaca tgtatgcgaa
     7561 aaatcctaat ggttggttat cctcaaaacc cccagcctgg tttgtggttg tttgctggtt
     7621 ttacccattc ttgtattggg tctttggtgt tttgcttttc atgataatgc ccaaatcttt
     7681 tggggtgctg tttatgagca acttgtatct gctctgcttt ccaattcctc ccgtaatgat
     7741 gcttttttat agcagt
//
LOCUS       DAOQNZ010000008         7656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3591_length_7656_cov_4.073280, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000008 DAOQNZ010000000
VERSION     DAOQNZ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3591_length_7656_cov_4.073280"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3100
                     /locus_tag="PKW73_00285"
     CDS             <1..3100
                     /locus_tag="PKW73_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71743.1"
                     /translation="LVKVQPIKASTQPLKMPVTEDFTHRYWESTQETLVGLYAKRDPI
                     TGQPVEGVNDIIVRVATSVAIAELKYALTPAELIELTIEEALVHPKVVQWAQIFADNI
                     GNQRFWANTPANINADPEVSLKVLQYWAYGKLANLKEEDIWLRSEELRLAVTQGKIAN
                     LNEQEVLMGHISTFLRGKGCLAACGVAYVIDSLEGIQEAARIEALAAKAAMGMGLNTS
                     SLRPWASIISNGAAASGPDRFYEKTIAKAVEAVAQGGRRGGALIELRNSDHPDILFFI
                     DKKKLFPPPTLSGVYQEMTRKFRQESGEDAMTYKKRLLKMAEQKFAEQYNQYIDRQNY
                     LKNTNVTVLAMPGFMEAVEAKRFIPAFFNKQVWGGSVYDPRKPVLDERTGQVKVNKLT
                     KEPIYEEYSVDITRFPEAIEAARKLTNAMVEITDKCVKVRGHFYAPEVFQRIVEGMRD
                     SGEPGIGFYETINEANANDHAYDLNTCNPCGEQFLPAGPGKDGRTYMGNCNLSSLHAA
                     HPEFWGPDGSYNMTAMRAVVKVQQRFMDNVSDVSWYPIPLQNMTARLERRNGGGFAGI
                     AEFLSRLGAEFGSAEGLNATEELFRQYNKASLEASMELAKERGCYPLWEGSKFQKKGL
                     RVRNTCMTNNAPTGTLAQALQTSWGVDPHNGIVFSRKVRSRFVDFVAPGFKELMIRYG
                     AWPESEDAQNALMKKIRANNKSCRGLAEVPEVVQKAFPIRVEVSPEAYIRHLSAIHIG
                     ADECPEVFNSVSNTCSIPLDFSEQAVHDSTMLAWKLGVKDITFYPDGSRLSQPVEKIA
                     EQDYNRSSDLLALLGHQERRNIQVDETVGQNYKVRVGSPDGGSTLHVSLNHEPSHPGE
                     LVEVYARMGKPGAIEAGLFEAVGRLASAFLQYAAEFGEEERSKAEMTVVRQLVNIQSG
                     YPAFFKFSDSDKAVVVQSPCDGLAKAIQHYRKTHSANAHHEDLANAEAALNLGAVPKA
                     AIANAEAQPVQMTIGLLDSLHDAANGGTRRQAACGKCGGESWLKIDGCNVCQSCGYSK
                     CG"
     gene            3328..4818
                     /locus_tag="PKW73_00290"
     CDS             3328..4818
                     /locus_tag="PKW73_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71744.1"
                     /translation="MARQEATSNTPHGQKWKGGRIFPPLCLAAVLALSAAPALSPARA
                     QYQNYTADYNTELAASRLMSKGYSQLNAGHYEAAISTLSQALRCGPTRYQADILAAIG
                     SAHRGLKQYPQAISALRESLKVNPREKSAYYTFAIVYYEQKRYDDALAALERYKMLAK
                     DHKEEKKADDFIKDIQCYRHLMPAQEALFAGRKLEAKELLLKAAQQDPSPVSGAVHRM
                     LAHAYRGMGNSEQAIVEGQMALQFEPRNKSINYVIGLCYKDLGKIDEAIAYVKRFAQA
                     ESDPNERQKAREFIQDLEDDRVKLSDTSNGLPDYFHLLLSQGQLHPWSQSRMPLKIFV
                     SDGTGMKGYRSNFPSFITKSLDTWVKASGNKLSYQIVDNAAQADIQVEWTEDYLFKEE
                     EGRKRAAAGITANQPEHNGNTPWNIGHSQIRIQTFDCLSGDDCTDDDMYSTCLHEIGH
                     SLGLCGHSANYSDIMYFGATNRQLPAATKRDKATMGKLYEHYQSRL"
     gene            4974..6347
                     /locus_tag="PKW73_00295"
     CDS             4974..6347
                     /locus_tag="PKW73_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71745.1"
                     /translation="MNHIPNYPIESNRQFNQIQNQYRGNFDNGFQIVDNSRVIRPTEN
                     NFERAGGYDNAVRNLTSYDHTNAHEAMNRAAKSGNGVAFIVGSESTRDTQKLLDQMTE
                     LKKQNPGMEFVYLDKDKINADPAAGKWREWIQKTTGGDNLAFTSLQSVKAGPDGKPTM
                     DRVVSTHWGGDIKDSLLDQNQYAKRFTGEHKDRFKFAAENPEAREGKPEGSGADAAKR
                     EADQTKPKEGSEAAQDVFGMSKAEREELHKPGNEEKLDRKNKELFNKAAEAARKAGLP
                     LVVNFEGRGCQPCLAKKNEANPELEKALDGKAVMLNMDASEEATREFLAGHGMEVNSW
                     PATGVFSVDKDNNLKLHGQLMGGYASRADLEKMPPEEVAQAMSLDRLQAGIAGSLPDA
                     GQEMQRLKNAQTREKERSRQEPGSGLENLSPDQLRNRIMNTLLASEGSKGRAMADLAG
                     AIERLAA"
     gene            6421..7401
                     /locus_tag="PKW73_00300"
     CDS             6421..7401
                     /locus_tag="PKW73_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71746.1"
                     /translation="MHFVVSRRIVFSLTLGLLICTGLASPAGAADKGRTGQEQVSDLV
                     HLNGTRLNIKAPAGMTKADNFAGFIDPNTNASLMVTEMPAPFKQLTEGFTDERLAARG
                     MQLVGKQSKEKGEYQGLEVDLTQKLYGTSVKKHVYIFGNDNFSIVATATLLEGTPDSI
                     MAKLREAIDSITIDTTSHPSLDEGLPYKLKETANLKRATRLQNTLLFNKAGKTNPPEK
                     GPLFLSSQSIMKVRIEQPDDFARRRIKDTPYVKDMEIEKEQDIELSGLKGRKLLARGV
                     DAKTAEKTFVYMILLFEKDGYYILQGISPESMRPIMEKEFDEISGSFKLL"
     gene            7467..>7656
                     /locus_tag="PKW73_00305"
     CDS             7467..>7656
                     /locus_tag="PKW73_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71747.1"
                     /translation="MPVQQLCRKRKQVIGALMAALALSTLSVAAVHAEEPFQLKAQNH
                     SEFRPATMSVAYLPPSRST"
BASE COUNT         2051 a         2243 c         1970 g         1392 t
ORIGIN      
        1 cctggttaag gttcaaccta ttaaagcaag cacccagccc ctgaagatgc cggtgaccga
       61 agatttcact catagatact gggaatcaac ccaggagact ctcgtaggac tgtatgccaa
      121 aagagacccc attaccggtc agccggtaga gggcgtcaac gacatcatcg tgcgcgtggc
      181 aacttccgta gccattgccg aactgaaata tgcactcacc cccgccgaac taatcgaact
      241 gactatagag gaagcccttg tccatcctaa ggttgtacag tgggctcaaa ttttcgccga
      301 caacatcggc aaccagagat tctgggccaa cacccccgcc aacatcaacg ccgacccgga
      361 agtatccctg aaagtattgc agtactgggc ctacggcaaa ctggccaacc tcaaggaaga
      421 agacatctgg ctgcgttcgg aagaactgag actggccgta acccagggca agatcgccaa
      481 cttgaacgag caggaagtgc tcatgggcca tatttccacc ttcctgcgcg gcaaaggttg
      541 cctggctgcc tgcggcgtcg cttatgtgat cgacagcctg gagggcatcc aggaagcagc
      601 ccgcatcgaa gctctcgcag ccaaggctgc tatgggcatg ggtctcaata cctccagcct
      661 gagaccgtgg gcttccatta tttccaacgg cgctgctgct tccggaccgg accgttttta
      721 cgaaaaaacc atcgctaaag cagtggaagc tgtggcgcag ggcggcagac ggggcggcgc
      781 tctgatcgaa ttgcgcaact ccgatcaccc ggacattctc ttcttcatcg acaagaaaaa
      841 gctcttcccg ccgcccactc tctccggcgt ctatcaagag atgaccagga agttccgcca
      901 ggagtcaggc gaagacgcca tgacctacaa gaagcgtctg ctcaaaatgg ctgaacaaaa
      961 attcgccgaa caatacaatc agtatatcga caggcagaac tacctcaaga acaccaatgt
     1021 caccgtactg gccatgcccg gtttcatgga agccgtggaa gcgaaacgtt tcattcccgc
     1081 cttcttcaac aagcaggtct ggggcggctc cgtctacgat cccagaaaac cggtgctcga
     1141 tgagcgcacc ggccaggtga aagtaaacaa gctcacgaaa gagcccatct acgaagagta
     1201 ctccgtcgat atcacccgct tccctgaagc aatagaagcc gctcgcaaac tgaccaatgc
     1261 catggtggaa atcaccgaca aatgtgtcaa ggtgcgtggt cacttctacg caccggaagt
     1321 cttccagcgc atcgtggaag gcatgagaga cagcggcgag cccggcatcg gtttctatga
     1381 aaccatcaat gaagccaacg ccaatgatca cgcctacgac ttgaacacct gcaacccctg
     1441 cggagaacag ttcttgcctg ccggtcccgg taaagacggc cgtacttata tgggtaactg
     1501 caacctctcc tccctgcacg ccgcgcaccc cgagttctgg ggtccggacg gcagctacaa
     1561 catgactgcc atgagagcgg tggtgaaagt acagcagcgc ttcatggaca acgtctccga
     1621 cgtgagctgg tatccgattc ctttgcagaa catgacggct cgtctggaaa gaagaaacgg
     1681 cggcggcttc gccggcatcg ctgaattcct ttcaagactg ggcgccgaat tcggcagcgc
     1741 cgaaggtctc aatgccacgg aagaactctt ccgtcagtac aacaaagctt ccctggaagc
     1801 ctcaatggaa ttggcgaaag aacgcggttg ctatcccctc tgggaaggca gcaagttcca
     1861 gaagaaaggt ctgcgcgtta gaaatacctg catgaccaac aatgccccga caggcaccct
     1921 ggctcaggct ctgcaaacca gctggggagt agatccccac aacggcatcg tcttctcccg
     1981 aaaagtacgc tcgcgtttcg tcgacttcgt cgcccccggc ttcaaagaac tgatgatcag
     2041 atacggcgcc tggcccgaaa gcgaagatgc gcaaaatgca ctgatgaaga agatccgcgc
     2101 taataacaaa tcttgccgtg gtctggcgga agtgccggaa gttgtgcaga aagccttccc
     2161 gattcgggtg gaagtatctc cggaagccta tattcgtcac ctttccgcca tccacatcgg
     2221 cgccgatgaa tgccccgaag tcttcaactc cgtctccaac acctgctcta tccccctcga
     2281 cttctccgag caagccgtgc atgattccac catgctggcc tggaaactgg gcgtcaaaga
     2341 catcaccttc tatccggacg gcagccgcct ttctcaacct gtagaaaaaa tcgccgagca
     2401 agactacaac agaagttccg acctgctggc tctgcttggg caccaggagc gtcgcaacat
     2461 ccaggtagac gaaaccgtcg gtcaaaacta caaagtgcgc gtcggctctc ccgatggcgg
     2521 ctccactctg cacgtcagcc tcaaccacga accgagccat cccggcgaac tggtagaagt
     2581 ttatgctcgt atgggtaaac cgggagccat cgaagccggt ctcttcgaag cagtcggtcg
     2641 ccttgcttcc gccttcctgc aatacgccgc cgaattcggt gaagaagaac gctccaaagc
     2701 agagatgacc gtggttcgcc aactggtcaa tatccagtcc ggctatccgg ccttcttcaa
     2761 gttctccgac tccgacaagg cagtagtagt gcagtcgccc tgtgacggac tagccaaggc
     2821 cattcaacac tacagaaaga cccactcggc aaacgcccac cacgaggatc tggctaatgc
     2881 ggaagcagct ctcaacctcg gagccgtacc gaaagcagcc atcgccaatg ccgaagcaca
     2941 gcccgttcag atgaccatag gcttgcttga ttccttgcat gatgccgcca atggcggtac
     3001 cagaagacaa gctgcctgcg gcaagtgcgg cggtgaatcc tggctcaaga ttgacggctg
     3061 caatgtctgc cagtcttgcg gctacagcaa atgcggttaa tagccaaaaa ctattgactg
     3121 cggccataga cttaagggga acgcccggga ctgcaccaaa caaacaagac cgtagcctcc
     3181 ccgagtaact ttaagaccct gcccatgagg cagggtcttc ggctatcttg gttcgactga
     3241 aaagcgggca tatgaaaaac acagccgcca gttggcagtg cgagcctgag aacctcagct
     3301 cacaaaccct agagaaagca tgtgtgaatg gcaagacagg aagcaaccag caacactccc
     3361 catgggcaaa aatggaaggg cggtagaatc ttccccccgc tctgcctggc cgccgtcctg
     3421 gcgctatcgg cagcgcccgc tctctcaccc gcccgggctc agtaccagaa ctataccgct
     3481 gactataaca ctgagctggc cgcctccagg ctcatgtcca aaggctatag ccagctcaat
     3541 gcaggccact acgaagcagc cataagcacc ctttcccagg ccctgcgctg cgggccgacc
     3601 cgctaccagg ccgatatcct tgccgccatc ggcagcgccc atcgggggct caagcagtat
     3661 ccgcaggcca tctcggctct gagggaaagt ctcaaggtca acccgcggga aaaaagcgcc
     3721 tactacactt ttgccattgt ctactacgaa cagaagcgct atgacgatgc cctggccgct
     3781 ctcgagcgct acaaaatgct ggccaaagac cacaaggaag agaagaaggc cgacgacttc
     3841 attaaagaca ttcagtgtta ccgtcatctg atgcccgctc aagaggctct ctttgccggt
     3901 cgcaagttgg aagccaaaga actgcttctg aaagccgccc agcaagatcc ttcccccgtc
     3961 tcaggggctg tgcaccgcat gctggctcac gcctatcggg gcatggggaa ttcggagcaa
     4021 gccatagtcg aaggtcaaat ggctctccaa ttcgagcctc gcaacaaatc tatcaactat
     4081 gtgatcgggc tttgctacaa agatctgggc aaaatcgacg aagcaatcgc ctacgtcaaa
     4141 cgctttgccc aggcggagtc agaccccaat gagcggcaaa aagccaggga attcatccag
     4201 gacctggagg acgatcgggt caagctgagc gatacttcca acggactgcc cgattacttc
     4261 cacttgctct tgtcccaggg gcagctacat ccctggtccc agagccgcat gccgctgaaa
     4321 atcttcgtct cggacggcac cggcatgaaa ggctatcgct ccaactttcc ctccttcatt
     4381 acaaaatccc tggatacctg ggtgaaagcc tccggtaata agctttccta ccagatagta
     4441 gataacgccg ctcaagccga catccaggtg gagtggaccg aggactatct cttcaaagaa
     4501 gaagaaggac gcaagcgcgc cgcagccggc atcaccgcca atcaacctga gcacaacggc
     4561 aatacaccct ggaacatcgg gcattcccag attcgcattc agacctttga ttgcctctcc
     4621 ggcgacgact gcaccgatga cgacatgtat tctacttgcc tgcatgaaat cgggcattct
     4681 ctcggacttt gcggccattc cgccaattat tccgacatca tgtatttcgg tgctacaaac
     4741 cgacaactac cggcggccac caagcgtgac aaagccacca tgggcaagct ctacgaacac
     4801 tatcaaagtc gcctctgagg gcgctgcaat aagcttgaaa caatttaccg ctaaattctc
     4861 agtatcgcca ggcggcgact ttaccactga ggcaagaggg cgcagaaatg ggctgaggac
     4921 cgcggcaaga ttgtggaccg gtcaggtacg aaccacaatt cgattcacag gttgtgaatc
     4981 acattcccaa ctatcccata gagagcaatc gccagttcaa ccagatacag aatcagtatc
     5041 ggggcaactt cgacaatggc tttcagattg tcgacaacag ccgcgtcata agaccgactg
     5101 agaataactt tgaaagagcc ggaggctacg acaacgccgt caggaatctc acttcatacg
     5161 accacaccaa tgcccacgaa gccatgaata gagcggctaa aagcggtaat ggagtggcat
     5221 tcatagtagg gtccgaatca acgcgcgaca cccagaagct gcttgatcaa atgactgaac
     5281 tgaagaaaca aaatccaggc atggaatttg tttatctgga caaggacaag atcaatgccg
     5341 atcccgcagc cggcaagtgg cgagaatgga tacaaaaaac caccggtggt gacaacctgg
     5401 ccttcaccag cttgcaatca gtgaaagccg gaccggacgg caaacccacc atggacaggg
     5461 tggtcagcac ccactggggc ggcgacataa aagacagcct gctcgaccag aatcaatacg
     5521 ccaagcgctt cacaggcgag cacaaggatc gcttcaagtt tgcagcggaa aacccggaag
     5581 cccgggaagg caaacccgag ggaagtggag ccgatgctgc caaaagggaa gcggaccaaa
     5641 ctaaacccaa agagggcagc gaggccgccc aagatgtgtt cggcatgagc aaagcagaac
     5701 gggaagaatt gcataaaccg ggcaacgaag agaagctcga ccgaaagaac aaagaactct
     5761 tcaacaaggc agccgaagca gcaagaaaag ccggactgcc gctagtggtc aattttgaag
     5821 gacggggctg tcagccctgc ctggccaaaa agaatgaagc caatccagag ttggagaagg
     5881 ctctcgacgg caaggccgtc atgctcaata tggacgccag tgaagaagcc acccgcgaat
     5941 ttctggccgg acacggtatg gaggtcaact cctggccggc taccggggtc ttctccgttg
     6001 ataaagacaa caatctcaaa ctgcacgggc agctcatggg cggatacgca agccgggcag
     6061 accttgagaa aatgccgccg gaagaggttg cccaggccat gagtctggac aggctgcagg
     6121 ccggcatcgc cggaagcttg cctgatgccg ggcaggaaat gcaacgcctt aagaatgctc
     6181 aaaccagaga gaaagagcgc tcacggcagg agcccggcag cggactggag aatctcagtc
     6241 ccgatcagtt gcgcaaccgc atcatgaata ctttgcttgc ttcagagggc agcaagggga
     6301 gagccatggc cgacctggcc ggagcgattg agcgactggc agcctagaat gcaattttgc
     6361 gtcaaaagcg gtataaattc ctgaggtcca gatccacatg aaaggctcca gggaaagaat
     6421 atgcattttg tcgtctccag aaggattgtt ttctccctca ccctgggctt actgatttgc
     6481 acaggtctcg caagcccagc gggggctgcc gacaagggcc ggaccggtca agagcaagtg
     6541 agcgacctgg tgcatttgaa cggtacccgg ctcaatatca aggccccggc cggtatgacc
     6601 aaagccgata actttgccgg cttcatcgat cccaacacca acgcctccct catggtgacg
     6661 gaaatgccgg ctcccttcaa acaactcacc gagggcttca ccgacgaacg cctggccgca
     6721 agaggcatgc aactggtagg caaacaaagc aaggagaaag gcgaatatca gggactggaa
     6781 gtagacctga ctcagaagct ttacggcaca agcgtcaaga aacacgtcta catcttcggc
     6841 aatgacaact ttagcattgt agcaacggca accctcctgg aaggcactcc cgacagcatc
     6901 atggccaagc tgagagaagc cattgatagt atcactattg ataccaccag ccacccttct
     6961 ctcgatgaag gactacctta caagcttaag gaaacagcca atctcaaacg ggcgacccgc
     7021 ctgcagaaca cactcttatt caacaaggcg ggtaaaacca atccaccgga gaaagggccc
     7081 ctctttcttt cctcgcaatc gatcatgaaa gtgcgcatcg agcagcccga tgacttcgcc
     7141 cgccgccgca tcaaagacac tccctatgtg aaagatatgg aaatcgaaaa ggagcaggac
     7201 attgaactga gcggcttgaa agggcggaaa ttgctggccc ggggcgtgga tgccaaaacg
     7261 gcggaaaaga ctttcgtcta catgatcctg ctcttcgaga aagacggcta ttacatcttg
     7321 caaggtatct cgccggaatc catgcgcccc atcatggaga aggaattcga cgagatcagc
     7381 ggcagcttca agctgctata agtggtaata taagcgctgc cggcggaacc atgcaacggc
     7441 ccactagaaa gaatcagaca aagacagtgc cagtccagca actttgccgc aaacggaaac
     7501 aggtcatcgg cgctcttatg gccgctcttg ccctcagcac tctctcagtt gctgccgtgc
     7561 atgctgaaga gccctttcaa ctgaaggcgc aaaaccatag cgaattccgc ccggcgacca
     7621 tgagtgtagc ctacctgccg cctagccgca gtacgt
//
LOCUS       DAOQNZ010000009         7607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3625_length_7607_cov_3.964115, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000009 DAOQNZ010000000
VERSION     DAOQNZ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3625_length_7607_cov_3.964115"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="surE"
                     /locus_tag="PKW73_00310"
     CDS             <1..679
                     /gene="surE"
                     /locus_tag="PKW73_00310"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNA71748.1"
                     /translation="APDRQRSATGHSLTLHKPLRVEHAQLDGPVAGAWSTTGTPADCV
                     KLAVSELLQEPPDVVISGINSGPNLGSDILYSGTVAAAMEAAFMDLPSIAVSARKGEP
                     AVYLTAARFISRFLTTFKQAPRSKRGLININIPAVPEAEIKGVKLCQLGVRLYNDTFE
                     KRSDPNGRDYYWLSGQAIEDTEAEDSDVFAVNSGYIAVTPVLFNMTDLDLMNRLDESK
                     QLSTLLK"
     gene            692..1426
                     /locus_tag="PKW73_00315"
     CDS             692..1426
                     /locus_tag="PKW73_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNA71749.1"
                     /translation="MMSEEVKASAVQDMTAVLSSITADKNWLMKVSIGSTVSYIALAI
                     FLLNPMFAPVSVVMGGITAGYLLRVMRQTINVVASRDETDGPLPEWNQILDLMISGVS
                     WLSFSLFFSFGGVILAFSLFTQGLQSRHSLVIDPHFTSWGTTTYLAIYSYYVLTSFFT
                     ALLMANFAEEEKMMAGFAWLKVIKRIAKAPVLMVTTWLASATLVFLAATVPSLTVIGV
                     IFAPFLTFLAQVIQARMLGFAWRAAG"
     gene            complement(1464..2045)
                     /locus_tag="PKW73_00320"
     CDS             complement(1464..2045)
                     /locus_tag="PKW73_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71750.1"
                     /translation="MRLNEEFVASFIVTLLASTFLFAPGSALAAPAKAVPAVKNEAAD
                     KNQGKQIAVSIPGLGEAKIVVTPAPAGAAQHGKPVAGKKTEAGIGPFGALSEGLKPGE
                     NISLKLPRSVNEFIDDMNGVGKRVGRSAQSGMTEVGKIAHWIAVYFKQFTGTPNLTPG
                     AYPYVETPSPSPGAHVAKVMLTPEGRVKTIVSR"
     gene            2608..3996
                     /locus_tag="PKW73_00325"
     CDS             2608..3996
                     /locus_tag="PKW73_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNA71751.1"
                     /translation="MNAFFVAAEMGLARVRRTRIDHLAEGGNMFAKKTQIYLEDPEKF
                     ISACQLGITIATLLLGAAGEAALAENIAEHAKHFGEQAAWGHKTIESLRWFSYAAAFT
                     FTAYVQTVGGELLPKMLTYTRAETVILWTVLPMHFWCILTSPFLKILNGTTAIIVALL
                     KIKEPPSAHTVHSEEELKMLVSASHEEGVLESEQEEMLHSVFDFADTTASEVMTPRID
                     MVCVPAEATVKAFVTEALKHGHSRLPVYDEDVDNIFGVVHIRDGLRALIEHKENAHVR
                     EFARKVLIVPENKNLKDLLTEFKRTKTHMAVVMNEYGGTQGIVTLEDLLEELVGDIAD
                     EHEIVEEFITQQEDGSYLIDAKLTLDEANEKLGLNIEDEEFNTIGGHVFGLLGREPQP
                     GDEVTSDLCVLTVVESDRHRIIKLRLVKTAPDTNESETTQILTQVNASIKPANGHNQE
                     ASGSHKSVEASH"
     gene            4000..4872
                     /gene="rsmA"
                     /locus_tag="PKW73_00330"
     CDS             4000..4872
                     /gene="rsmA"
                     /locus_tag="PKW73_00330"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNA71752.1"
                     /translation="MAFSITREDLYRRAMVVRAKKRLSQNFLVEPSILSMIADSINAG
                     PGDTVVEIGPGLGFLTEFLVRTGARVFAVELDEDMAFRLKDLAAEFPNLTIIRQDFLH
                     FDPESLKAKNIKVVGNVPYQITSPIVVRVLGELDEPGIWLPYVESLTMTVQLELARRL
                     VSPPGSKEYGQITILADYYATAKMIGKVGSEHFIPRPDVESAVVQLVKRERPKVTVKD
                     ASLLRRLVKAGFKERRKMIKNNLGFTHLNQEELTSLLMAHGISPAARAEGLSLDKFAQ
                     LADALKAYALDEND"
     gene            4865..5884
                     /gene="ispE"
                     /locus_tag="PKW73_00335"
     CDS             4865..5884
                     /gene="ispE"
                     /locus_tag="PKW73_00335"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNA71753.1"
                     /translation="MTEKVVKVEAPAKVNFTLELLGLMEDGYHRVKTLMQTVSLSDTL
                     TFKISPAREPSIELSVEGKPASDFPLSQDNLICKAVRLFLSSNLDPEKAALNPLKISV
                     HVEKRIPIGAGTAGGSGNAAAALLALNEYFDRPFTRESLAEMGKKLGADVPFSLLGGT
                     MVGLGRGDELQSIGAEENFCPLYLVLAKSRNLSVPTVWAYKTYDECTEVFGLSERTKD
                     RGITDDATAYAAEKLAAGEITDAIKVFGNDFEQAIFPHYKELKAIKQRFMQEGALTSH
                     MTGSGPTVYAVCRTQEEAEKLKRDYEEGVLRETTISCAAKSDPVDLFVCMTTPHGARI
                     LDNES"
     gene            5944..6894
                     /gene="nth"
                     /locus_tag="PKW73_00340"
     CDS             5944..6894
                     /gene="nth"
                     /locus_tag="PKW73_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNA71754.1"
                     /translation="MGQVKKTRTANTKATTKASAKAAAKVSTKESARATTTANTKATT
                     KASTKAAAKSTGKVTGKTTGKVTDKTTGKAKGKTTGKAKGKTTGKATGGQKSAPRGVS
                     KKGSHKATRQEAAAIMKGLLELYPDACCELTYDSDFQLLTAVILSAQCTDQQVNKVTK
                     ALYAEFPDARSLAEADLERVKTLIKPTGYYNAKAKNIQACAQMLVTRFNGKVPTTVEE
                     LTTLPGVGRKTANVVLGVIHNIPGWSVDTHVQRLSKRFSFTNEEDPLKIEKDLQALFP
                     GEDWSKLSITIIWHGRRLCYARKPACHNCPINTICPASEV"
     gene            7017..>7607
                     /locus_tag="PKW73_00345"
     CDS             7017..>7607
                     /locus_tag="PKW73_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71755.1"
                     /translation="MTNLGTIKTIGVLLAVSIPAKQVLRSIFILQVFPLLAGVTFHGS
                     FWQALLISLSAFALPFLTASAVMWFVIARLLLLNLLVKRINPIFAEKAQLSFWLRSSK
                     DSEATKLKLENRSPMASVKSSLAPKRCLMTATYYLLVLATFAKFGSFLQFSSLSALGS
                     ATLACTVFDIAMALVTDLVFSYWYTRNSDQEAEKRAC"
BASE COUNT         1968 a         2103 c         1923 g         1613 t
ORIGIN      
        1 cgcaccagac cgacaacgca gtgccaccgg gcattcactc acactgcaca agccgctgcg
       61 ggtcgaacat gcccagttgg atggtcccgt agccggcgcc tggtcaacca ccggcacccc
      121 cgccgactgc gtcaaactgg ccgtctccga acttttgcaa gagccgccgg atgtggtaat
      181 ttccggcatc aactccggtc ccaacctggg ctccgacatt ctctactccg gcaccgtggc
      241 agccgccatg gaagcagctt tcatggacct gcccagtatt gccgtcagcg ccagaaaggg
      301 agagccggcg gtctatttga cggcagcccg tttcatcagc cgcttcctga cgaccttcaa
      361 gcaggcgccg cgcagcaaaa gagggctgat caatatcaat attccagccg taccggaagc
      421 ggaaatcaag ggcgtaaagc tctgccagct tggcgttcgg ctctacaatg acactttcga
      481 aaagcgctcc gaccccaatg gtcgagacta ttactggcta tccggtcagg ccatcgaaga
      541 caccgaagca gaagattccg atgtctttgc tgtcaatagc ggctatatag ccgtcactcc
      601 cgtgctcttc aacatgaccg atcttgacct gatgaacagg ctcgacgaaa gcaaacagct
      661 ttccaccttg ctcaaataag cagggcttaa gatgatgtct gaagaagtaa aagcaagcgc
      721 agtgcaagat atgaccgcgg tattgagtag catcaccgcg gacaaaaact ggctcatgaa
      781 ggtcagtatc ggctccacag tcagctatat cgcccttgct attttcctgc tcaacccgat
      841 gttcgcccct gtttccgtag tcatgggcgg catcaccgcc ggctatcttt tgcgggtgat
      901 gcgccagacc ataaacgtag tcgcaagcag ggatgaaact gatggtcccc tgccggagtg
      961 gaatcagata ctcgatctga tgatatcggg agtaagctgg ctttccttca gtcttttctt
     1021 cagcttcggc ggcgtgatcc tggccttttc cctcttcacc cagggactgc aaagccgcca
     1081 cagtctggtc attgatcctc atttcaccag ctggggcacc accacttatc tagccattta
     1141 cagctattac gtgctgacga gtttcttcac agccctcctc atggccaatt tcgccgaaga
     1201 agagaagatg atggccggct tcgcctggct gaaagtaata aagagaatcg ccaaagcacc
     1261 cgtgctcatg gtcaccacct ggctggcttc cgccaccctg gtcttcctgg ctgccaccgt
     1321 gccatcgctc acggtgatag gcgttatatt tgcacctttt ctcacctttc tggcccaggt
     1381 aattcaagcc agaatgctgg gttttgcctg gcgagcggcc ggctaaagac tgatcaaata
     1441 aatatcggta agataaatac cggtcaacgg ctgactatgg tcttgacgcg accttccggc
     1501 gtgagcataa ccttggctac atgggctccc ggcgaggggc tgggagtctc aacataagga
     1561 taggctccgg gggtgagatt gggagtgccg gtaaattgct tgaagtagac ggcaatccag
     1621 tgagcgatct tgccgacctc ggtcatgccg ctttgagcgc tgcgaccgac tcttttaccg
     1681 acaccgttca tatcgtcgat aaactcattg actgaacggg gcagtttgag agaaatattt
     1741 tcgccgggct tgaggccttc actgagcgca ccgaagggac cgatgccggc ttcggtcttc
     1801 ttgcctgcca cgggcttgcc gtgctgagcg gcaccggcag gcgccggggt gacaacgatt
     1861 ttggcctccc ccaatccggg gatagagact gcgatttgct tgccctggtt tttatcagcc
     1921 gcttcgttct ttacagcggg caccgctttt gcaggagccg ccagagcgga cccaggggca
     1981 aacaaaaagg ttgaagccag aagagtcact ataaaggaag caacgaattc ttcatttaat
     2041 ctcataaatc aggtccatgc cgcctacagt aaattgccgg gggaaggtcg atgtccttgg
     2101 gggctttcca tcagattcgg agcgcctttg ctacagttct caggaaagaa ccttgctccc
     2161 gccaaatgtc agattatgtc atagctcaga gctaagacag agaagcccca ggtaatttct
     2221 tgataacctt ggcaaagcac cgccggctgg gaaatcttac acttggcaag cattaaaatt
     2281 gtgtattgga ctaagagagc atatggtagg attgatctaa ctctggaatc ccatggctgt
     2341 tatcagcctt tcgattgagt gagactgcct ctcaaatcga aatagagttg tctctgttgc
     2401 aggcagatta agagaccaat tagcactttg atagacgatc ctgacagtag cagccagaca
     2461 ggtgatgaat cagaagaacc tccccagggg tgcaaggcga aaccttttgc gtgccaccag
     2521 gaagttcagc caaaggagac aggttcagag ttaatctgag cctttattgc tttgtaccaa
     2581 attgcctgga taccaatact gattttaatg aacgccttct tcgtggcggc ggaaatggga
     2641 ctcgcccgtg tcagacgcac acgcatcgat catctggccg aaggcggcaa tatgttcgcc
     2701 aagaagactc agatctattt agaagaccct gagaagttta tttctgcctg ccagcttggc
     2761 atcaccatcg ccaccctctt actgggtgcg gccggcgaag cggccctggc tgaaaacatt
     2821 gccgaacatg ccaaacactt cggcgagcaa gccgcctggg gtcacaaaac catagagagc
     2881 ctgcgctggt tttcatacgc cgcggcattt accttcacag cctatgtgca aaccgtaggc
     2941 ggcgaactgc tgcccaaaat gctcacttac accagggctg aaacggtcat actctggacc
     3001 gtactgccca tgcacttctg gtgcattttg acctcgcctt tcctgaagat cttgaacggc
     3061 accacagcca taattgttgc ccttctgaaa atcaaagaac cgccttccgc tcacaccgtt
     3121 cactcggaag aagagctgaa gatgctggtt tccgccagcc atgaagaagg tgtgctggaa
     3181 tcggaacagg aagagatgct gcactcggtc ttcgattttg ccgacaccac ggcaagcgaa
     3241 gtgatgacgc cgcgcatcga catggtctgc gtaccggctg aagccacggt aaaagctttt
     3301 gtaaccgaag ccctcaagca tggtcattcc cgcctgcctg tctacgatga agatgtagac
     3361 aatatttttg gcgtggtgca cattcgagac ggcttgcgag ccctcatcga gcataaagaa
     3421 aacgctcatg tgcgcgaatt tgccagaaaa gtattgattg tgccggaaaa caaaaacctg
     3481 aaagacttgc tgacggaatt caaacgcacc aagactcaca tggccgtggt catgaacgaa
     3541 tacggcggca cccagggcat agtcacactc gaagatctcc tggaagaact ggtgggcgac
     3601 attgccgatg agcacgaaat agtagaagaa ttcatcaccc agcaagagga cggctcctac
     3661 ctcatcgatg ccaaactcac ccttgatgaa gccaacgaaa aactggggct gaacattgaa
     3721 gatgaagagt tcaacaccat tggtgggcac gtcttcggac ttttagggcg ggagccgcaa
     3781 ccgggagatg aagtgacatc cgatctctgt gtattgacag tggtagagtc ggatcgccac
     3841 cgcattatca aacttcgact ggtcaaaacc gcacccgata caaacgaaag cgaaacaacc
     3901 cagattttga cccaggttaa tgcctccatc aagccagcca atggtcataa ccaggaggcc
     3961 tcaggcagcc ataaatcagt ggaggcaagc cactagctaa tggctttttc aattaccagg
     4021 gaagatctct accgccgggc catggtggtg agagccaaga aaagactgag ccaaaatttc
     4081 cttgtggaac caagcatcct ttctatgatt gccgactcca tcaatgccgg tcccggcgac
     4141 accgtggtgg agataggacc aggtctcggc tttctcacgg agtttctggt gcgcaccggc
     4201 gccagagttt tcgccgtaga actggacgaa gacatggctt ttcgcctcaa ggatctagcc
     4261 gccgaatttc ccaacctgac cattatcagg caagactttc tgcactttga tcctgagagc
     4321 ctgaaagcaa aaaatatcaa agtggtaggc aatgtgccct atcaaatcac ctcccccatt
     4381 gtggtgagag tgctgggtga actggacgag cccggcatct ggcttcctta tgtggaaagt
     4441 ctgaccatga cggtacaact ggagttggcc aggcgcctgg tatcaccgcc cggcagcaag
     4501 gagtacggac aaattaccat cctggccgat tactatgcca ccgccaaaat gatcggcaag
     4561 gtcggctcgg aacacttcat tccccgcccg gacgtggaat cggcggtggt gcagctggtc
     4621 aaacgggaac gaccaaaagt aaccgtaaaa gatgcatcgc tcttgcgtcg gttggtcaaa
     4681 gccggcttca aagagcggcg caagatgatc aagaacaatc tcggctttac tcacctgaat
     4741 caggaagaat tgacttctct gctcatggcc cacgggattt caccggccgc cagagcggaa
     4801 ggactctccc tggataaatt cgcccaacta gccgatgccc tcaaggccta tgccctggat
     4861 gaaaatgact gagaaagtag taaaggtaga agccccggcc aaagtcaact tcacccttga
     4921 gctgctcgga ctcatggaag acggctacca ccgggtaaag accttgatgc aaacagtctc
     4981 cctgagcgac actctcacgt tcaagataag ccctgctaga gaaccatcca tcgaacttag
     5041 cgtggaaggc aaaccggcaa gtgactttcc cttaagccag gacaacttga tctgcaaggc
     5101 cgtccgcctc tttctaagca gcaatcttga tcctgagaaa gcggccctta accccctgaa
     5161 aatttcggtg cacgtggaaa aacgtatacc gataggagca ggcaccgccg gtggctccgg
     5221 caatgccgca gccgccctgc ttgcgctcaa tgaatacttt gaccgcccct tcaccagaga
     5281 aagtcttgcc gagatgggca aaaagctcgg tgccgacgtg cccttttcac ttctgggcgg
     5341 caccatggtc ggtcttgggc gcggggatga actgcaaagc atcggtgcgg aagaaaactt
     5401 ttgtccgctc tacctggttc tagccaagag ccgcaacctc agtgtaccga cggtctgggc
     5461 ctataaaacc tatgacgaat gtactgaagt ttttggactt tccgaacgca ctaaagatcg
     5521 cggtatcacc gacgatgcca cggcctatgc cgccgaaaag ctggctgctg gtgaaataac
     5581 ggatgcaatt aaagtcttcg gcaatgattt cgaacaggcc atctttcctc actacaaaga
     5641 gctgaaagcc atcaaacaac ggtttatgca agaaggtgcc ctcacctccc atatgaccgg
     5701 ctcgggacca acggtctacg ccgtctgccg cacccaggaa gaagcagaaa agctgaagcg
     5761 cgactatgag gaaggggtat tgagagaaac taccatttct tgtgccgcca agtcagaccc
     5821 ggtagatctc tttgtctgta tgaccacacc tcatggtgcc agaattctag acaacgagag
     5881 ttaaaccgac taaccggaga ataccggcta acaacaagtt acggcggcga ggagaaaagc
     5941 cagatgggcc aggtgaagaa aaccagaacg gcgaacacaa aagcaaccac taaggcaagc
     6001 gctaaagcgg ccgcaaaggt gagcacaaag gaaagcgcca gagcaaccac aacggcgaac
     6061 acaaaagcaa ccactaaggc aagcactaaa gcggccgcaa aatctaccgg taaagtgact
     6121 ggcaaaacta ccggcaaagt gacggacaaa actaccggca aggcgaaggg caaaactacc
     6181 ggcaaggcga agggcaaaac taccggtaag gcgaccggcg gacaaaagag cgccccccgg
     6241 ggggtcagta agaagggctc tcacaaagcc accaggcaag aagcggccgc catcatgaaa
     6301 ggtttgcttg agctttatcc cgatgcctgc tgcgaactga cctacgatag tgattttcaa
     6361 ctgctcacgg cggtaatact atcggcccag tgtactgacc agcaggtaaa caaggtcacc
     6421 aaagcactct atgcagaatt tcccgatgcc cgctccctgg ccgaagccga cctggagcgt
     6481 gtcaaaacct tgattaaacc caccggctat tacaatgcta aggccaagaa cattcaagcc
     6541 tgtgcccaga tgctggtcac cagattcaat ggcaaagtgc ccaccacggt ggaagagctg
     6601 actaccctgc ccggcgtcgg tcgcaaaacc gcaaatgtcg tcctcggcgt catccacaac
     6661 attccgggct ggagcgtgga tacccatgtg cagcggcttt cgaaacgctt cagtttcacg
     6721 aacgaagagg acccgctcaa aatcgagaag gacttacaag ccctctttcc aggggaagac
     6781 tggtccaaac tttccatcac cataatctgg cacggcagaa ggctttgcta tgccaggaaa
     6841 ccggcttgcc acaattgccc catcaacacg atctgtccgg cgagcgaagt ttgaggacta
     6901 agtcctggcg gcggaaagcc atagaagttg aaggatttta gaacatactg cattgcaatt
     6961 tcagctaaag cctatactgg gatcagtacg acttaaagcc tgaaagtgta gcaacagtga
     7021 caaacttagg cacgataaaa acaataggag tcctactggc agtatccatt ccagcaaagc
     7081 aagtactgcg ttccattttt atcttacagg tcttcccgct cctcgctggc gtcaccttcc
     7141 atggttcctt ctggcaagct cttctcattt ccctttcagc ctttgccctg ccctttctca
     7201 ccgcttcggc ggtgatgtgg tttgtcattg cgcgcttgct tctccttaat ctactggtca
     7261 aacgtatcaa tcccatcttc gccgaaaaag cccaactgtc cttctggctg agaagcagca
     7321 aagattcgga agcgaccaaa ctgaagctgg aaaatcgcag cccaatggcg tcggtcaaaa
     7381 gctcactggc gcccaagcgc tgccttatga cggcaaccta ctacttgctg gttctggcaa
     7441 catttgccaa atttggttcc ttcctgcagt tttcatcatt gtctgctctg ggctcagcca
     7501 ctctcgcctg cacagtcttt gatatcgcca tggccttagt cacagacctg gtgttcagct
     7561 actggtatac aaggaactca gaccaggaag cagaaaagcg cgcctgc
//
LOCUS       DAOQNZ010000010         7562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3667_length_7562_cov_3.414946, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000010 DAOQNZ010000000
VERSION     DAOQNZ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3667_length_7562_cov_3.414946"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKW73_00350"
     CDS             <1..473
                     /locus_tag="PKW73_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNA71756.1"
                     /translation="LLDLSGRVVGINTAIFSGTRQSAGIGFAIPINIAKNIIPQLIEF
                     HRVLRPDMGVDVIAEEGGGLRVMRLTRGGPAAAAGLQGPRVLVYRYGELEWRQIERGF
                     ADLIVAVDNVQVNSVDSLMSYIESKKPNQVVTLTVIRANRPLKIPVKLTVGASD"
     gene            537..1256
                     /locus_tag="PKW73_00355"
     CDS             537..1256
                     /locus_tag="PKW73_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA71757.1"
                     /translation="MSRILVIEDDLAIAELVKINLELLGHQVVTAPDGIKGLAMAQQN
                     RPDLIVLDVMMPDLDGFTVCQRLRQNPQTNPIPVLMLTALSTTKDKVAGFDAGADDYL
                     PKPFDTPELQVRVRALLRRAGSVPHSATLPEILTAGEITLIPENLQAKVGGRIEKLTP
                     TEFEILHCLMQHHGQTVSTGKLLEEVWGYAPDDDVDTIRVHIRHLRTRLEKGDRKYIK
                     TVYGGGYQLVAEGFTKEKGSE"
     gene            1522..3009
                     /locus_tag="PKW73_00360"
     CDS             1522..3009
                     /locus_tag="PKW73_00360"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA71758.1"
                     /translation="MLFNSLQYLVFLPTVFFLYWVLPQKARVPLLLVASYVFYCSWRP
                     IYGLLIFGLTLANFLIVPYIDKAAKAEGGKHVFSPKVWLGITIAVNLITLGIFKYAYF
                     TMDTIKGGLGLFGVSWNEPHLHIILPLGISFFVFEFIHYAMEVYRGKPVVKSFSALAL
                     FASFFPTQIAGPIKRYQDFVPQLTEKIKFKWEYLDEGMQLILMGLCKKVLLADNLALV
                     VQAGFAHPENFSSVDMWLITYAFAFQIFFDFAGYTDIARGSALLFGYKVPINFNLPYL
                     AANVADFWHRWHISLSTWLRDYLFIPLGGSRGSKWLNYRNLFITMALGGLWHGAAMHF
                     LAWGAYQGIVLILHKEYVRLLDAIGVGKQLKESPALAKVYHVFSVIATFHIVCVGWVF
                     FRAEDMKTAGVIITKLLQVPADLMHFSAAQLAVLQIRDPIIFPALLVLLPILMIAQPV
                     VNWLNDKTIYKNPPWALQVSVMVALMCIITIFSPDSSPSFIYFQF"
     gene            3056..4342
                     /locus_tag="PKW73_00365"
     CDS             3056..4342
                     /locus_tag="PKW73_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71759.1"
                     /translation="MAKTDHSLDVDSGPAVNVLVQEKLEASYLASQKEGTKRSKSSFL
                     AALALFAVLSVGLSLNQFEKLKPGDFPVNTWTTWAISDFLQEKQKNDVVFLGSSLMLV
                     PLDGVDADFLGKPIDASAHHRSLYFEDAFKKDSGHSLKSYNFALPGEMPSDAYLITDF
                     LLKQEKAPKVLVYGVGPRDFMDNLLPSPAATDPYRYLSRFGSLESHAGLLMPDWQERF
                     NYELTRLCYIYGNRGQLALDVNRGLKAFLDRAAKRPEVMAEADRVALRRKLLPDYLPY
                     EVKRKECFFRPTTEATRGGFQDNVAEYRKRYKTLKMSTFTTQMQFLKDLIAIANERGT
                     HVVLVAMPITQVNRDLLPDSSWALYRNSLREVAAAGKSVTFHDMHETGKFKLSDFQDT
                     VHLHSGGGAKLLFEVSKLMSADAATKRALGLTDGTP"
     gene            4339..5766
                     /locus_tag="PKW73_00370"
     CDS             4339..5766
                     /locus_tag="PKW73_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71760.1"
                     /translation="MKRRLSAAITAITAFLFLNLALSFFAPIKFNPFNYPYQGWGWWS
                     FHDLRASQEIHNVALLGSSLMVAAVNNCDANYLNRPLNLTEHHRSFYLDDKLEATFKG
                     KFRSYNLAGPGQMPSDAYLTLKGMLSTAPRPDVVIYGVAPRDFIDSQLSSPTDTEPFH
                     FLSRLVSTDDCAGGLFRDPLGRLSWFINRNLYFAHHAIDWQMLLERKTNRVLSILTPL
                     PYGSKPYTFWQRTALLPKYKAGEIHPNAVITHPVTKEVAKATFKDNTGEYKERYKRAD
                     RHTYATQLYFLGKLANLCKKERIELILINMPIARENINVLGAPTYLDYVSTLKERAYA
                     LNIPFYDLNFFYLYNTDLYHDYVHLNAFGGAKFLDSLVSVLNSDPRAQLAMQMAGREL
                     WKQEQMNKLATPVNDATDSIHRLKEKLIEHNLIQTSAGHGSISKLREIVKQEGDRLRR
                     QIGARLNKGKEGNEQDLDDEPGVAM"
     gene            5766..7052
                     /locus_tag="PKW73_00375"
     CDS             5766..7052
                     /locus_tag="PKW73_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71761.1"
                     /translation="MASEKQTTSKRKVAFTWAVALFAMVNVALYSYMTAQGNKFGKRE
                     LTLEALRATNYEKRTEGPWVWWVTKTFLSQPRTPDVVLLGSSQMGSAIFSAEAEHRGA
                     IVDTTDDRQVTRLAAELARAGTGKPEVFNFSMGGAMVSDHYLMANTLFKGEHKPKVAI
                     IGVNPRDFIDNNLPSASATDTFHFLSGYADLEKLAPVSFDDSFGYLDYLINRHLPLKK
                     ANLLVYGDLPPSLGTKPSFTNPEGKGAVKSTEKKPVTTQVLRAISGSAGDVKKGEWVL
                     PAFPPYLYMDNSKEYIKRYKNEHPRCAEGQRRYFEELLSFLADQDIKVIVVGMPSQAC
                     NRQLLSQGFWTEFRGFLARTTQNHGGTYVDLFDDKRFLAGKDYLDTVHLNRWGGGKLV
                     KAIAEATASRADIVASLKNKQGTAIGGKPGAERKWQ"
     gene            complement(7073..>7562)
                     /locus_tag="PKW73_00380"
     CDS             complement(7073..>7562)
                     /locus_tag="PKW73_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71762.1"
                     /translation="PFPLSWAYKALFSNHNSQAQLAAMRAETLFRLGRLKESLKLMEE
                     AIAGSPSGYKNQLIYARYLYEDGQGEKARTVLNEVLEKHKDVLMPRIYDLALVTGDNA
                     AAEQHKIYDESMKVLDEQVFGPEPAWPPGGERPIMEMWRRDDLEFLSRRLLFSQPAKP
                     GS"
BASE COUNT         1829 a         2261 c         1826 g         1646 t
ORIGIN      
        1 ccctgctcga cttgtccggg cgcgtggtag gtataaacac agccattttc agcggcacga
       61 gacaatcggc gggaattggc ttcgccattc ccatcaacat tgcaaaaaat ataatccccc
      121 agttaatcga gttccatcgg gtgctgagac cggacatggg cgtcgatgtg attgccgaag
      181 aaggcggcgg gctcagggtt atgcgcctca cccggggcgg accggcagcg gcagccggac
      241 ttcagggtcc gagggtgctg gtgtaccgct acggtgagct ggaatggcgc cagatagagc
      301 ggggcttcgc cgatttgatc gtggccgtgg acaatgtcca ggtcaactcc gtagactcac
      361 ttatgtctta tatagagagc aagaaaccaa accaagtggt tacactgaca gttatcaggg
      421 ccaaccgtcc gctcaaaatt ccagtaaaat taacagtcgg agcttccgac tagtttccag
      481 aatctggaaa tagatgcaac ggcaggctag gcttaacaaa gcaggcggag aaaacattgt
      541 caagaattct cgtcattgaa gacgatttgg ccatcgctga attagtaaaa atcaatcttg
      601 agcttctcgg gcatcaagtt gtaacggcgc cggacggcat caagggtctg gccatggccc
      661 agcaaaatcg tcccgattta atcgtgctcg acgtcatgat gcctgatctc gacggtttca
      721 cggtctgcca gaggctcagg caaaatccgc agaccaatcc cattcctgtc ctcatgctta
      781 ctgctctttc caccactaaa gacaaagtgg ccggcttcga cgccggtgcc gacgactacc
      841 tgcccaaacc cttcgacact cccgagttgc aggtacgcgt gcgcgccctc ctgagacggg
      901 ccggcagtgt gccccattcc gccacgctac ccgagatcct cacggcaggt gaaattacac
      961 tcattcccga gaatttacag gccaaggtgg gcggtcgcat cgaaaagctc acaccgacgg
     1021 aatttgaaat tctccattgc ctcatgcagc accacggaca gacggtttcc accggcaaac
     1081 tgctggagga agtctggggt tatgcaccgg acgacgatgt ggacaccatt cgggtacaca
     1141 tcaggcattt gcgcacccgc ctggagaaag gcgatcgcaa gtatataaaa accgtatacg
     1201 gtggtgggta tcaattggtg gcggaaggct tcaccaaaga aaaaggcagt gagtaatatt
     1261 tcactttgtc ttttattagc gtcctgtcac tcaagcttgc caagcgccaa aaatcaagcc
     1321 accataagcc tcaaagcgaa ttatcaaagg cttactataa acgttttagg ttaagagaag
     1381 cgccttaagc tggacatatt ggaaaccctc cctatatcat gtgcgatgag cgattggaaa
     1441 cattttgctg gggcctcaaa tctgttagtt aggcaacttg cttgaagcag agcgccccga
     1501 gtcagttcaa ggagcaccac cttgctattc aacagcctgc agtacctggt ctttctaccg
     1561 acggtattct ttctctactg ggttctcccg caaaaagcgc gggtgccact gctcttagtg
     1621 gctagctatg tgttctattg ttcctggaga ccaatctacg gtcttctgat tttcggcttg
     1681 accctggcca acttcctgat cgtgccttac atcgataagg ctgccaaagc agaaggcggc
     1741 aagcatgttt tcagccccaa agtgtggctc ggcataacca tcgccgtcaa cttgatcacc
     1801 ctgggcattt ttaaatacgc ctacttcacc atggacacca tcaagggcgg tctcggactg
     1861 ttcggcgtca gctggaacga gccccacttg catatcatcc tgcccctggg tatctccttc
     1921 ttcgtcttcg aatttattca ctacgccatg gaagtgtaca ggggcaagcc tgtagtcaaa
     1981 tccttctcgg ctctggccct ctttgccagt ttcttcccca ctcagatagc cggaccgatt
     2041 aagcggtacc aggacttcgt gcctcagttg actgagaaaa tcaagttcaa atgggaatac
     2101 ctggatgaag gcatgcagct catcctcatg ggtctttgta agaaagtgct tctggccgac
     2161 aatctggccc tggtggttca ggcaggcttt gctcatcccg agaatttctc gtcggtggat
     2221 atgtggctta ttacctatgc cttcgccttc cagatcttct tcgactttgc cggttacacc
     2281 gacatcgccc gcggttcggc cctcttgttc ggctacaaag taccgattaa cttcaacctg
     2341 ccctacctgg cagccaacgt agccgacttc tggcaccgct ggcatatcag cctctccacc
     2401 tggttgagag actacctctt cattcccctg ggcggttcaa gaggctccaa gtggctcaac
     2461 tacagaaacc tcttcatcac catggctctg ggcggtctct ggcacggtgc cgccatgcac
     2521 ttcctggcct ggggcgccta tcaaggtatc gtgcttatcc ttcataagga atatgtacgc
     2581 cttctcgacg ccatcggcgt aggcaagcag ttgaaagagt ctccggctct cgccaaggtc
     2641 taccatgtct tctcagtcat cgccaccttc cacattgtct gtgtgggctg ggtattcttc
     2701 cgggctgaag acatgaagac tgccggcgtc atcatcacca aacttctgca agtgcccgcc
     2761 gacctgatgc atttcagcgc cgcccagcta gccgttctgc agatccgtga cccgatcatc
     2821 ttcccggccc tgctcgtgct cttgcccatc ctcatgatcg cccaaccggt ggtcaactgg
     2881 ctcaatgaca aaaccatcta taaaaatccg ccctgggctc tccaggtgag tgtgatggta
     2941 gcgctcatgt gcataatcac catcttcagc cctgacagct cgccgtcctt catctacttc
     3001 cagttctagg aagtgatacc gcccatggcg gccattggca attcgggagc atttcgtggc
     3061 aaagaccgac cactctctag acgttgatag cggccccgcc gtaaacgttc tggtgcaaga
     3121 aaaactggaa gccagctatc tggcctccca gaaggaaggc accaaaagaa gcaagagcag
     3181 cttcctggcc gcacttgctc tctttgccgt cttgagtgtg ggtctttcac tgaatcagtt
     3241 cgaaaaactc aaaccaggcg attttccagt caacacctgg accacctggg ccatttccga
     3301 cttcttgcaa gagaagcaaa agaacgatgt ggtcttcctg ggttcctccc tcatgctggt
     3361 gcccctcgac ggagtagacg ccgacttcct gggcaaaccc atcgatgctt ccgcccacca
     3421 tcgctctctc tattttgagg atgccttcaa gaaagacagc ggtcacagcc tcaaaagtta
     3481 taacttcgcc ctgccggggg aaatgccctc cgatgcctac ctcattaccg actttctcct
     3541 gaagcaggaa aaagctccca aagtgcttgt ctacggtgtc ggtccgagag acttcatgga
     3601 caacctgctg ccctctccgg ccgccacgga tccttaccgt tacctctcac gtttcggttc
     3661 cttagaaagc cacgccggtt tgcttatgcc tgactggcag gaacggttca actacgaatt
     3721 gaccaggctc tgctacatct acggcaaccg cggtcagctt gccctggatg tcaatcgagg
     3781 gctgaaagcc ttcctcgata gagccgctaa aagaccggaa gtaatggcgg aagccgaccg
     3841 cgtggccctg cgtagaaagc ttctgcccga ctacctgcct tacgaagtaa agcgcaaaga
     3901 atgcttcttc cgcccgacca ccgaggccac ccgtggcggc tttcaggaca acgtggcgga
     3961 atatcgcaag cgctataaga cgctcaaaat gtccaccttc accacccaaa tgcaattcct
     4021 gaaagacctg attgccatag ccaatgagcg cggcacccac gtagtgctgg tagccatgcc
     4081 catcactcag gtaaaccgtg atctcctgcc tgacagttcc tgggcacttt atagaaacag
     4141 tctccgggaa gtagcagcag ccggtaagag cgtcaccttt catgacatgc acgaaaccgg
     4201 caagttcaaa ctgtccgatt tccaagacac tgtccacctg cactccggcg gcggcgccaa
     4261 gctgctcttt gaagtctcaa aactcatgtc tgccgatgcc gccacaaaac gagccctggg
     4321 cttgacggac ggtacaccat gaaacgccgt ctcagcgcag ccataaccgc catcaccgcc
     4381 tttctctttc tgaacctggc cctatctttt ttcgccccca taaaattcaa ccccttcaac
     4441 tatccttatc agggctgggg ctggtggagc tttcatgacc tgagagcgag tcaggaaatt
     4501 cataatgtag ccctgcttgg ttcatcactg atggtggcag cggtcaacaa ctgtgatgcc
     4561 aattatttaa atcgccccct caacctgact gagcaccacc gctcgttcta cttagacgac
     4621 aaactggaag ccaccttcaa aggcaagttc cgcagctaca atctggccgg tcccgggcaa
     4681 atgccctccg atgcctacct gaccttgaaa ggtatgctca gcacggcccc ccgtcccgac
     4741 gtagttattt acggcgtggc cccccgcgac tttatcgaca gccagttgag cagccccacc
     4801 gacaccgagc ctttccattt cctcagtcgc ctcgtctcca ccgatgactg cgccggcgga
     4861 ctcttccgcg atcccctcgg cagactctcc tggttcatca acagaaacct ttattttgcg
     4921 caccacgcca tcgactggca aatgctactg gagcgcaaaa ccaatcgcgt cctctccatt
     4981 ctgacgcctc tgccctacgg cagcaaacct tacacgttct ggcaacgcac cgccctgcta
     5041 cctaagtata aagccggcga gattcacccc aatgccgtca tcactcatcc cgtcactaaa
     5101 gaggtggcaa aagccacctt caaagacaat accggcgaat acaaagagcg ctacaagcgc
     5161 gccgacagac acacctatgc cacccagctc tattttctgg gcaaactggc caacctctgc
     5221 aagaaggagc gcatagagct tatcctgatc aatatgccta tcgccagaga aaacataaat
     5281 gtgctgggcg cccccaccta tctggattac gtcagcactc tgaaagagcg cgcctacgcc
     5341 ctcaacatac ccttctacga cctcaacttc ttctatctct acaacaccga cctctaccat
     5401 gactacgtgc acctgaacgc cttcggcggc gccaagtttc tcgatagcct ggtatccgtc
     5461 ttaaactccg acccgcgcgc ccaactggcc atgcaaatgg ccggtcgtga gctgtggaag
     5521 caagaacaga tgaacaaact ggccacgccg gtaaatgatg caaccgacag tattcatcgc
     5581 ttgaaagaga aactaattga acacaatctg attcagacca gcgctgggca cggctccatc
     5641 agtaaactga gagaaatagt caaacaagaa ggtgatcgcc tgcggcggca aatcggcgcc
     5701 cgcctcaaca agggcaaaga aggcaacgag caagaccttg atgatgagcc gggggtagca
     5761 atgtaatggc ctccgagaag caaaccacaa gtaaaagaaa agtagccttc acctgggccg
     5821 tggctctctt tgccatggtg aatgtcgcgc tctattccta tatgactgcg caaggcaata
     5881 aattcggcaa gcgcgaattg actttagaag cactgcgagc caccaactac gagaaacgga
     5941 cggaaggtcc ctgggtctgg tgggtcacca aaactttcct gtcccagccc cgcacaccgg
     6001 atgtggtact actgggcagt tcccagatgg gctcggccat attttccgcc gaggccgaac
     6061 accgcggcgc catagtcgat accaccgatg acagacaggt caccagactg gctgccgaac
     6121 tggccagagc tggtacaggc aagcccgagg tattcaattt ttccatgggt ggtgccatgg
     6181 tatcagacca ctacttgatg gctaataccc tcttcaaggg agaacacaaa cccaaagtgg
     6241 ccatcatcgg cgtcaatccc agagacttca tcgacaacaa tctgccctcg gccagtgcca
     6301 ccgatacctt tcacttcctc tccggttatg ccgatcttga aaaactggca ccggtttcct
     6361 tcgatgacag cttcggttat ctggattacc tgatcaatcg ccacctgccc ctcaaaaaag
     6421 ccaacctgct ggtatacggc gacctgccgc cctcactggg aaccaagccc agcttcacaa
     6481 acccggaagg caagggagca gtgaaatcca ccgagaaaaa gccggtgaca acccaggtat
     6541 tgagggccat ctccggttca gccggtgatg tcaaaaaagg cgaatgggtc ttgccggcct
     6601 tcccgcccta cctctacatg gataattcga aagaatacat caaacgctac aagaatgagc
     6661 atccacgctg cgcagaggga caacgtcgct actttgaaga actcttgagc ttcctggccg
     6721 accaggacat caaagtaata gtagtgggta tgccttctca ggcatgcaac cgccagcttc
     6781 tctcccaggg gttctggact gaattccggg gtttcctggc ccggaccacc caaaatcatg
     6841 gaggcactta tgtggacctc ttcgacgaca aacgcttcct ggccggcaaa gattacctcg
     6901 acacggtgca cttgaaccgt tggggcggcg gcaaactcgt gaaagcaatc gccgaagcaa
     6961 cggcaagccg cgccgatatc gtggccagcc tgaagaacaa acaaggcacc gccatcggtg
     7021 gcaagcccgg cgccgaaaga aagtggcagt aaaccctcag gcgagagccg ctctaagagc
     7081 ccggcttggc cggctgtgaa aacaacagtc gccgggagag aaactccaga tcatcccgtc
     7141 gccacatctc cataatcggt ctttctccgc cgggcggcca ggccggctcc ggaccgaaga
     7201 cctgctcatc aagcaccttc atgctctcat cgtaaatctt atgctgctca gctgcagcat
     7261 tgtctcccgt gaccagagcc agatcgtaaa tgcgcggcat caagacatct ttgtgcttct
     7321 cgagcacttc attcaagacc gtgcgggctt tctcgccctg cccgtcctca tataaatagc
     7381 gagcataaat tagctgattc ttgtagccgg aagggctgcc ggcaatggct tcctccataa
     7441 gcttcaaaga ctcctttaaa cggcccagac gaaagagggt ctcagcccgc atggctgcta
     7501 attgtgcctg agaattatgg ttggaaaaga gagccttgta ggcccaggaa agaggaaagg
     7561 gg
//
LOCUS       DAOQNZ010000011         7481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3726_length_7481_cov_4.611769, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000011 DAOQNZ010000000
VERSION     DAOQNZ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_7481_cov_4.611769"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="PKW73_00385"
     CDS             <1..1289
                     /locus_tag="PKW73_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71763.1"
                     /translation="QTITVTGPVVVGETVQFTVFNIDLPGSLKTVSYVAQAGDNLNQI
                     RCGLRTQVNNDADLAEVGIHAACGGGVLTLTSNSEEQTFYRAAAEIQVACEPITTSLT
                     LFPRDKARYCFSTVTPVGFPQYQNIMLQTLISIGQSGTDAAFRLSISDSTWFQFTNHN
                     DYYLVKDSIPQPANAPRFYNDENFGASQVGDTVTVQIGAATVYTSAIFENTSPDGAMQ
                     DRIVAHVTAHETGHHLDHIYNGGNIDGYSKTATFQAALTRDVTGMNAANACTIEISDP
                     ALPGEPPVLYDGIFTNMLDSHGNYVCGAPGNAGRTPIYGGSNVEIAQQAWPRLYPNLP
                     DPDDQDAELFAEEAAVVLGFPDTVDNNGNQINGSSYGLNGIPFMCTYEVVRTLIQDGA
                     PTITGIGYVAPIGSGPNGTVHKCDGTTEVVRIFPGS"
     gene            complement(1363..2250)
                     /locus_tag="PKW73_00390"
     CDS             complement(1363..2250)
                     /locus_tag="PKW73_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71764.1"
                     /translation="MYMPRFQITLVLLLVSFLQSSNLSFASTEGAVASSVKEIEKPFS
                     ANWPAERPPGESLPVFRSYNCGGGSRPPLYFAYWIHSYFEPKNLSEKAKAQFRLEIKT
                     KEWKNWYKLATANCTPADIFYIEQVIWEASPFACTNGATSSGKWSLSHKGVEFITAQQ
                     RLEKKVKPLYEPTVAGSPKNDVLGRIKSGEGLCNNFSCETLYRNEHPDVTKEIAVVHF
                     VPLSAAQCFPTAIEQNELHSLKNLKALEIDKCNSAQLDSFRDEWTQFLKKPANTMSRQ
                     MVLKEADRLAAKYHSLFLN"
     gene            complement(2440..3033)
                     /locus_tag="PKW73_00395"
     CDS             complement(2440..3033)
                     /locus_tag="PKW73_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71765.1"
                     /translation="MSEAAQPINVDLISTDGLEPLFEATEPGSETENQTVEPGSEPIS
                     GSAQEIVSTDEAARRLGISSRAVIKRLQTGSLQGFRDTTKTRSEWRIYWKEPSSEPVN
                     KSKNIQTEPERTGTEERTTPKEPSGTGSDSYLIELNKKLLEQVQALTYRNGYLQSQLV
                     EREQDIAEQEEKIKLLTDSQYKRGWWARFSSWFMTGK"
     gene            3539..5416
                     /locus_tag="PKW73_00400"
     CDS             3539..5416
                     /locus_tag="PKW73_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71766.1"
                     /translation="MDAQISGVVSDEGRGLFRGCYWSHKTRHSVFDIDIDSRYRNGAE
                     LKKLQAHVAAVDLYAISYRSSNSGGWHLYLPFDEWAESDEVNQTLKTWLKSLGYEITA
                     GQLEVFPSGNALRLPLQKGFAWLNSDGSIKVRREEISEDEALASFLQDFESNARNWQQ
                     AKTLIESQIQSAGRSAGAGVQEHEKVISIAGFDDLFRKGKIQEKWDKGRDYWLHGLTA
                     KNQRHDAIQAVGHYLWYGDPLANVPAYPGRRNNETRARLIEAWLRKKHNGQCNHINRG
                     HWAAIEADIWRAVFWRRQQGYQVYSPYPLTDRLLKRLLEVYNKTGLLFEIDRMAEANN
                     SRLEDARSRIKKAIKELLEEGVVLTKAEIARRAGANRRTVAKHSDLLTIWPSVNSRGV
                     WGVPLPLVGCNETVGFLSRILTGPEIGFEEKEKSEIPLSSNSDSGDLKPACETPGLGI
                     NGTKSSAKEIPLEVAPLQFCPANSQPESTQHKDRALRVATQVFTPGPLLSGIRAEGQM
                     CAGGISFISAAGAGRQSCYTVRVTGCGIKEKAGAASIASALSGFGEESNFEKIIPDTT
                     NYRFLTTDKPGYSSGGTDEFSLISKRKRNTTVLKNSSSVRLAARRPTLWLRGPPGIDS
                     S"
     gene            5782..6195
                     /locus_tag="PKW73_00405"
     CDS             5782..6195
                     /locus_tag="PKW73_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71767.1"
                     /translation="MIEKKERKFKPTVETKLAQADSNRVGALAKKEGKTKSEVVREAV
                     LWYLAHKEELEFQDREALIAKEIRGMTDRICGMLARQGAAIGTLYELTWSSLPNEDAK
                     EQFRSAMNTAKQKMRNRLEKDERDMADAMKKVVAP"
     gene            6192..>7481
                     /locus_tag="PKW73_00410"
     CDS             6192..>7481
                     /locus_tag="PKW73_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71768.1"
                     /translation="MIVVRHKYIAGRGPRARGGKVVAIGRALQHVKYIQHRPGRDREP
                     GGREMFNEREDSIDAREMRKAIKELGGSRVIVHKVTLAPEISPADKKAFTREVMINLS
                     QEKGRDLQWFAVEHGNTEHPHIHVVVLGRDKHGTEVQISLKDIEKVKEYGDRFLERHH
                     PREFERARQDRERKERERLEARNKERELEKAERIRDGLELPWMNRKIVREQLEPYKEW
                     KEKQKLEREERRKEKERSKEEREEKPYHQDTIEAAGRQWSRADRLAELRELNQHLWDN
                     YEDRIGKDEYKKLVGWIKDKEKYGDQPPEKQPEKEEKERDYFEHQNKRYSKDDSYEKL
                     TGLSRQLRERKEKLPIEDYQNLRGWIEDKDRQRWAGAIEKQVEIEKAKYARDEKNYAA
                     GQRAIDPLQQDLMRNPVIGLFMVEASIASELVRSIPLD"
BASE COUNT         2100 a         1682 c         1978 g         1721 t
ORIGIN      
        1 ctcagacaat cactgttacc gggccggttg tggtgggcga aacagttcaa ttcaccgtct
       61 tcaacatcga ccttcctggt tcactgaaaa cagtaagcta tgttgcgcaa gctggcgaca
      121 atctaaacca gatcagatgt ggtttgcgaa cccaagtaaa caacgatgca gatcttgcag
      181 aagtagggat ccatgctgcg tgtgggggcg gagtgcttac cctgacttca aattcggaag
      241 aacagacatt ttaccgtgca gcagctgaaa tccaggtcgc ttgtgagcca attaccacca
      301 gtctgacttt gttccctcgc gacaaggcaa gatattgctt ctcgactgtc acgccagtcg
      361 gattcccgca gtaccagaac atcatgcttc aaaccctcat ctccataggg cagtctggca
      421 cagatgcagc gtttcggctt tcaatcagtg acagcacatg gttccagttc acaaaccaca
      481 atgactatta cctcgtgaag gactctattc cccagccagc caatgctccg cgattttaca
      541 atgacgaaaa tttcggggca agtcaggtgg gagatactgt caccgtgcaa attggcgcag
      601 ctactgtgta tacatctgca atctttgaaa acacgtcgcc tgatggtgca atgcaagacc
      661 gcattgttgc tcatgtcacc gcacatgaga ctggtcacca tttggaccat atctataatg
      721 gtggcaatat agatggttat tctaaaacgg ctactttcca agccgcacta acacgcgacg
      781 tcacaggtat gaacgccgcc aatgcatgca caatcgagat ttctgatcct gctctaccgg
      841 gcgagccacc agttctgtat gatggaatct ttaccaacat gctggattcg cacggaaatt
      901 atgtttgtgg cgcaccgggg aatgcaggtc gtacaccgat ctatggtggt agcaatgtcg
      961 aaattgcaca gcaggcttgg ccgcgtcttt atcctaattt gcccgaccca gacgaccagg
     1021 acgctgaact atttgcagag gaagcggcag tagtacttgg tttccctgat acagtagata
     1081 acaacggaaa tcaaatcaat ggtagtagtt acggtctcaa cggcattccg ttcatgtgca
     1141 catacgaagt agttagaact ttgatccaag acggtgcgcc taccattaca ggtattggct
     1201 atgttgcacc gatcgggagt gggccaaacg gcaccgtgca caaatgtgat ggaacaacgg
     1261 aggtagtgag aatcttccca ggatcataaa aatcctgaag tactaaatgg acttggcagc
     1321 agttggtgaa tcaaatacta gctgctgctt cttttttgtc ctttaattca gaaatagtga
     1381 gtgatatttg gctgccagcc ggtccgcttc tttgagaacc atttgccgac tcattgtatt
     1441 tgcgggtttc ttgagaaact gtgtccactc atcacggaat gagtctaact gagctgaatt
     1501 gcatttatct atctctagcg ccttcaaatt tttgagacta tgcaattcat tttgctcaat
     1561 ggctgtagga aagcactgtg ctgcgctcaa aggaacaaaa tgcaccacag caatttcttt
     1621 tgtaacatca ggatgctcat tacggtagag ggtttcgcaa gaaaagttgt tgcaaagacc
     1681 ctcgccgctt tttattcggc cgaggacatc attttttgga cttcctgcaa cagtcggttc
     1741 gtaaagtggt ttcacctttt tctcaagccg ctgttgcgcc gttatgaatt ccactccttt
     1801 gtggctaaga ctccactttc ctgatgaagt cgcaccatta gtgcaagcaa aaggagacgc
     1861 ctcccaaatt acctgttcaa tgtaaaaaat gtccgctggt gtgcaatttg ctgtcgcgag
     1921 cttgtaccag tttttccatt ccttagtctt tatctcaagc cgaaattgtg cttttgcttt
     1981 ttctgagagg ttctttggtt caaaatagct gtgaatccag tatgcaaagt aaagaggagg
     2041 tctgcttcct cctccgcagt tatacgatcg gaagaccggt agagactcac caggcggccg
     2101 ttcggctggc caatttgcgg aaaatggttt ttctatctct ttgactgatg acgctactgc
     2161 tccttctgtt gaggcaaaag acagattgct cgattgaagg aaagatacca gcaacagaac
     2221 cagggtgatt tgaaatctcg gcatatacat gtccattcca tgagtaggct ttgtttagag
     2281 gttttgccca actgtgtcgg tactctttca tgcattacat catgtttgct taatgagcga
     2341 aagaacccag ttctgcttgc ttttgtggca tttctattat tgtatgtcgt aataacgatg
     2401 caacataaca accggttacc agacactcgg atgtggccgt tatttgccgg tcatgaacca
     2461 ggagctaaac cgagcccacc agccccgctt atactggctg tccgtcagta gcttgatttt
     2521 ctcttcttgt tcagcaatat cttgttctcg ctcgactaac tgtgattgca aatagccatt
     2581 tctgtaggtg agagcttgca cctgttcgag cagcttcttg tttagctcaa taagataaga
     2641 atcggaaccg gttccacttg gttcttttgg ggttgttctt tcttcggtac cggttctttc
     2701 tggttccgtt tgaatgttct tgcttttatt caccggttcc gaacttggtt ctttccagta
     2761 aatgcgccat tctgagcggg tttttgttgt atccctaaag ccttgtaggg aacctgtttg
     2821 aagtcttttg ataacagctc tggaagaaat ccccaatctc ctagctgctt catcagtgga
     2881 aactatttcc tgagctgatc ccgaaattgg ttcggaaccg ggttccactg tttggttctc
     2941 tgtctcggaa cctggttccg ttgcttcgaa caatggttcc aatccatcag tcgaaattag
     3001 atccacattt attggttgag ctgcttcaga cacggaatgt ccctttgtgt gattacatgg
     3061 gcattatgag acttcgtgcc accacctgta aaggcgtgat atatccactt ggcgggcgtg
     3121 tggatatccc gaaattcaaa caggctgctt gaattcaggc ttttaccgtc gtaggatcaa
     3181 ctatgggtta tcagatatgc cacttgcaat ggtggcacca aaaaaggaga agagcgaccg
     3241 cgcagaagtc ttaaaaagcc gaaaaatcag cttggcggct gatttaggcg gctggagccc
     3301 gaaacttatg aatcgatctg acagttgaat cataagtgat tgggttccta gggtcaagtg
     3361 cgtccttttt tcagggattg accgttaggc tttgaactcc attgctttga gagcgtccga
     3421 aaggacgcca gatcttttgg atctacgaca gaaatttttg tcgttgtatc cgcgccgcga
     3481 ttctttcatc tggaagacat atgaccagga gcgttggcag aaggctacag gcccgctctt
     3541 ggacgctcag atttctggcg tggtgtcgga tgaagggcgc ggcttatttc ggggctgcta
     3601 ctggtcgcac aagacccggc attccgtctt tgatatcgat atagacagca gatatcgaaa
     3661 tggagccgag ctaaaaaaac tgcaggcgca tgtagctgca gtcgatctat acgcaatttc
     3721 gtataggagc agcaattcag gcggctggca cttgtatttg ccgtttgacg aatgggcaga
     3781 aagtgacgaa gtcaatcaaa cattgaagac ctggctgaaa agtttgggat atgaaattac
     3841 cgccggacag ctcgaggtat tcccgagcgg aaatgcacta aggctgccgc ttcagaaagg
     3901 ctttgcctgg ctcaattcgg acggaagcat aaaagttaga agagaagaaa ttagtgaaga
     3961 cgaggcgcta gcctcattcc ttcaagattt tgaaagtaat gccagaaatt ggcagcaagc
     4021 gaaaaccctg attgaaagcc aaattcagtc cgccggcaga agtgccggcg ccggcgtcca
     4081 ggagcacgaa aaagtcatat ctattgcggg ttttgatgat ctgttccgaa aaggcaagat
     4141 tcaagagaaa tgggacaaag ggcgtgatta ctggcttcat ggactgacag ctaagaacca
     4201 aaggcatgat gccattcaag cagtcggtca ctatctctgg tatggcgacc ctttagcgaa
     4261 tgtcccagca tacccaggac gaaggaacaa cgaaaccaga gcgcgtctta tcgaggcctg
     4321 gctcaggaag aaacacaacg gccaatgcaa tcacatcaat agaggccact gggcagcaat
     4381 agaggctgac atctggcgtg ctgtgttttg gagaaggcaa caaggctacc aggtatattc
     4441 accctacccc ctaacagatc gcctcttaaa acggctccta gaggtctata acaagaccgg
     4501 tttgctgttt gaaatagaca ggatggccga ggccaacaat agccgtctgg aagatgcaag
     4561 aagccgaatc aagaaagcga taaaagagct gcttgaggaa ggagtcgtac ttacaaaagc
     4621 tgaaatagca aggcgggctg gggccaatcg ccgcacggtt gccaagcact cagacctttt
     4681 gacaatctgg cccagcgtta atagccgggg ggtgtggggg gttcccttgc ctttggttgg
     4741 ttgtaatgag accgtcgggt ttttgtccag aatccttacc ggcccggaaa ttggttttga
     4801 agaaaaagag aaatcagaaa ttccgctcag ttcgaattcg gactcagggg atctcaaacc
     4861 ggcttgcgaa actccgggtt tggggatcaa tggaacgaaa agtagcgcta aggaaattcc
     4921 acttgaggta gccccccttc aattctgccc ggcgaatagc caaccagaga gcacccagca
     4981 taaagaccga gccctgcggg ttgctacgca agtctttacg ccggggccct tgctctccgg
     5041 tattcgggca gaggggcaga tgtgtgcggg gggaatttct tttatctccg cagctggagc
     5101 cgggcgtcag agctgctata ctgtgcgggt caccggatgc ggtatcaaag agaaagcggg
     5161 cgcagcttca atagctagcg ccctgtctgg ctttggggaa gaaagcaatt tcgaaaagat
     5221 aatccccgac actactaatt atagattctt gacaacggac aagccaggct attcatcagg
     5281 cggaacagat gaattttcgc tgatctcaaa acgcaaacga aataccaccg tcctgaaaaa
     5341 ctcaagtagc gtaaggcttg cagccaggcg accgaccttg tggctcaggg ggcctcctgg
     5401 tatagacagc tcatgaatag aagctttcga gaaaacagct ctaacatgga gccgcatggg
     5461 cttaaaaccg acggtgccgc aaaagctaat actgccggac atctacagat tcggcggtgg
     5521 agggataagc cgaaaagggc acaatagggc atttttccag aaatgctgta aacagccacc
     5581 agttgccatt tttaagcgac gacggctcct tgcaacgaag ccgcgtaaga ggcaagatga
     5641 aagaaccggc tcacattcgt gagcggttct aaagagcgct gtgtacgcgc cgactaaaga
     5701 agagcctaga cacgaaagca caacaaatag tcgactcatg atatatgtag tattaatggc
     5761 tggttgtaag gagccgactg tatgattgag aaaaaagagc gcaaattcaa gccaacagta
     5821 gagaccaagc tggctcaggc agactcaaac agagtgggcg cactggcaaa gaaagaagga
     5881 aagacgaagt cagaggtagt aagggaggca gttctctggt atctggctca caaggaagag
     5941 ctggaattcc aagacagaga agcgctaata gccaaggaaa tcagaggaat gactgatcga
     6001 atctgcggca tgctggcacg gcaaggagca gccataggga ctctgtatga gctgacctgg
     6061 tcgagtctgc cgaatgagga cgcaaaagag cagttcagat cggcaatgaa cacagccaag
     6121 cagaaaatgc ggaatcggct tgagaaagac gaaagagaca tggcagacgc catgaagaag
     6181 gtagttgctc cttgattgta gtaaggcaca agtacattgc tggaagagga ccgagagcca
     6241 gaggcggcaa agtcgtggca atcggcagag cacttcagca tgtgaaatat attcagcatc
     6301 gccctggacg tgaccgagag ccaggcggcc gggagatgtt caacgaaaga gaagacagca
     6361 ttgacgcccg agagatgcgg aaggcaataa aagagctggg aggcagccgg gtcattgtcc
     6421 acaaagtgac gcttgctccg gagataagcc cggcggacaa gaaagcgttt acgcgggaag
     6481 tgatgatcaa tctgagccag gagaaggggc gcgacctgca atggtttgca gtcgagcatg
     6541 gcaacacgga acaccctcac atccatgtgg ttgtcttggg cagagacaag cacggaacag
     6601 aagtgcagat tagcctgaag gacatagaga aggtcaaaga gtatggggac aggtttcttg
     6661 aaaggcatca tccaagagag tttgagagag caaggcaaga ccgcgagaga aaagaaagag
     6721 agcgcctgga ggctcgaaac aaagagcgcg aattagagaa agcggagcgc ataagagacg
     6781 gtctagagct gccctggatg aacaggaaaa ttgtccgcga gcagcttgag ccgtacaaag
     6841 aatggaagga aaaacagaag ctggagcgag aagaaaggcg aaaagagaaa gagcgcagca
     6901 aggaagaacg agaagagaag ccatatcacc aggacacgat agaggcagcc ggacggcaat
     6961 ggtcgagggc tgacagactg gctgaactca gagaattgaa tcagcatctt tgggacaact
     7021 atgaggaccg gataggaaaa gacgagtaca agaaactggt ggggtggata aaggacaaag
     7081 agaagtatgg agaccagccg ccagaaaagc agccagagaa agaagagaaa gagagagact
     7141 atttcgagca tcaaaacaag cgctacagca aagacgacag ctacgagaag ctgacagggt
     7201 tgtcgcggca gctcagagag cgcaaagaga aattgccgat tgaggactat cagaatctca
     7261 gaggctggat tgaagacaaa gacaggcaac gctgggcagg tgcaatcgag aagcaagtag
     7321 aaatcgagaa agcaaagtat gcacgtgacg agaaaaatta tgcagccggg cagcgtgcaa
     7381 tagacccgtt gcaacaagac ctgatgcgga atccggtgat tggcctcttt atggtggaag
     7441 catcgatcgc gtcggaactc gttcgatcga tcccactcga t
//
LOCUS       DAOQNZ010000012         7321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3839_length_7321_cov_4.144922, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000012 DAOQNZ010000000
VERSION     DAOQNZ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3839_length_7321_cov_4.144922"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1347
                     /locus_tag="PKW73_00415"
     CDS             85..1347
                     /locus_tag="PKW73_00415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA71769.1"
                     /translation="MVRILVTAVGSDGDINPMIEIARALLSRGHDVRFIANGSFAHKA
                     EEVGLPFLPLGDSSLFQEVVSDPDLWHPRKGFSAVWRNINQSLPLSLELLEQNMEPCN
                     TILVGTTLALACRVLQEKSANSVPLATVHLSPACLISRYDPPQGPVSIISPQTPQFLK
                     DLYLSLVEVSMLENVCRDDVNRFRARFGLAPVKRIFARWLNSPDLVVLAFPEWYAQRQ
                     KDWPENLCYTGFPLFDRKQEQRLSDATRTFIDAGAAPVVFTAGSAMAHSRDYFSHALK
                     CVRARNIRAIFVTRYPEVLPRHLPDNVLHASYEPFDHLFAEASVVSHHGGIGTSAQCL
                     RAAVPQLVCPFAHDQFDNARRLEALGVAREIRVEKPKQWAGKLGELLDDPEVKVSCRK
                     VKELMTVCPDPAARAAEIIEGLAKKLRK"
     gene            complement(1328..1486)
                     /locus_tag="PKW73_00420"
     CDS             complement(1328..1486)
                     /locus_tag="PKW73_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71770.1"
                     /translation="MAEDKQPATLENFILKKNPWLALTMGIIFGAFFHYVIFRLSLPT
                     EPFIYAAF"
     gene            complement(1489..2667)
                     /locus_tag="PKW73_00425"
     CDS             complement(1489..2667)
                     /locus_tag="PKW73_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71771.1"
                     /translation="MTEKKSYSKYFLLGTMLGFLLSTAAGAYITGKPLPLSNFVRFHE
                     YINPNGGFYLTYNQMLAQARLLLADKPGRPLLIIGGDSVFYGEGQSSEHCWTKYLQQE
                     LGDDFVVINLALPGTKVFEAGYWVYEKLLSEGQNVFLLTDAMPSTVFEPSGTTPLHYM
                     YFDARERGMLLPYAARETFSRQHREWTLFKDEGHQENLELRAKLNHYLGVEELWTQFT
                     YSVAGNYFHRTTEFRSLLPRRLFQDPFEDRPPFEASDNEKLEHGTNIVKRFMAGITAL
                     YEKGPPSEYEQFFWRRLSGCTQASVPPNARNNLIVCVTAINPLFIDELNEREKKEYRH
                     IKELWLENFTRQGFQTWSLNDKLAPEHYVDFDHLSVEGGKAMATELAKRLKEFRERKN
                     "
     gene            complement(2660..3817)
                     /locus_tag="PKW73_00430"
     CDS             complement(2660..3817)
                     /locus_tag="PKW73_00430"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA71772.1"
                     /translation="MVKLRLSALLIFCAVFHSHFAGAAGVAPIAVLALLTYVTALTAS
                     KFKKGGGWLCLSTIIIVTAALLTYKYSRFLLLDVLASVNKPLAEHFAASLFTALPATP
                     PLAISFFAFEFIHYLYEVRRGREPIKKPQDFLAFAIFFPSLVAGPIKRYQDFIPSLYQ
                     GLSAVSSRDVAAGMVRLAAGFFKKLMIADNINQYILEKQAFYFAVDLPERWLFLLALA
                     GRIYYDFSGYTDIALGLAMMMGIKLPPNFNGPYGATSIRDFWQRWHMSLSFWIRDYIY
                     IPLGGSQHGLARRILSGILAFALCGLWHGASWNFLFWGLYHGLGLAINTVYRKLPWLS
                     PLWKLYDKYPLTARLTTFLFVVFGWLPFFYPLDKALSMFLMLFQFKGVSGD"
     gene            complement(3913..4734)
                     /locus_tag="PKW73_00435"
     CDS             complement(3913..4734)
                     /locus_tag="PKW73_00435"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNA71773.1"
                     /translation="MTLHLDILDRKNIEDALTTELIGRGVCELHDTIDSTNSRAAVLA
                     REGAPAGSLIVARQQTAGRGRQGRTWISPPDAGIAMSILLRPEIPLTRLPLITLATGV
                     AVAQAVEALCGIQLGLKWVNDLTAGGKKVGGILAEMPGQALVIGIGINLRLDEGNIPD
                     DLKNRIDWLENLAGRPLEPSLLVAEICRFLERQYFSLLEGKSQAIIDSWKHYSVTLGK
                     EVMVVSPTGDSARNISGRAVDIAESGALLIEKTDGTRVELSAGEISLRSLDGSYC"
     gene            complement(4779..5192)
                     /locus_tag="PKW73_00440"
     CDS             complement(4779..5192)
                     /locus_tag="PKW73_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNA71774.1"
                     /translation="MAKVAVKAKVGEGYRQEISAGSHAFASDAPKEFGGGESAPNPHE
                     LLLGSLGACTAITVQMYAKRKEWDLKSITVEVLEEPVVAEDGSKQTRILRSIELTGDL
                     TPEQVENLKSIANKCPIHKLLEGPKQIATEVAHKS"
     regulatory      5811..5947
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6072..>7321
                     /locus_tag="PKW73_00445"
     CDS             6072..>7321
                     /locus_tag="PKW73_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNA71775.1"
                     /translation="MTQVLTAVHHADPAGFGLSDERIGQFALYKVLRRNGNVVEFDPS
                     KIAVAMTKAFLAVEGTHGRESSRVRDLVNKLTGQVVETLMRRLPEGGVLHIEHIQDQV
                     ELAMMRAGEQEVARGYVLYREARAKDRAARGAEVPQPVQAGPNVKRTDGTMVPLSQLG
                     LNAIVKDACAGLGAVVEPESIYTATIESLYEGVQETEVVRSAIMSARALIETDPAYSF
                     ATARLLLHQMRLEVLGEGVSQEGMKERYATYFPEYIAKGIKVGLLDPRLATFDLEKIS
                     NALCAERDLKFQYLGLQTLYDRYLLHQEGIIFELPQAFWMRVAMGLSINEIDRETRAI
                     EFYNLLSNFDFVSSTPTLFNSGTHRPQLSSCFLTTVDDDLSSIYEAVRENALLSKFSG
                     GLGNDWTPVRALGAHISGTNGKSQG"
BASE COUNT         1625 a         1828 c         2018 g         1850 t
ORIGIN      
        1 acttgtgtcc cggtgccgat gtacctggca tgattgagca tcagttatcc aatgggcgga
       61 ccgtgatgtt aaggtgggac tgaattggta aggatactgg taacggcggt aggtagcgac
      121 ggcgatatca atcccatgat tgaaattgcc agggcgcttt tgtcgcgcgg ccatgatgtg
      181 cgtttcattg ccaatggcag ctttgctcac aaggcggagg aagtaggctt gcccttcctg
      241 cctctgggag acagttcact atttcaggaa gtcgtatctg acccggatct ctggcatccg
      301 cgcaaaggat tttcggcggt ctggcgtaat atcaatcagt ctttgccttt gagtcttgag
      361 cttctagagc aaaatatgga gccctgcaat actattctgg tcggcaccac tctcgcttta
      421 gcttgccgtg ttctgcaaga gaaaagtgct aactctgtgc ctttagctac cgtgcatctc
      481 tctcccgcct gcctgatttc gcgctacgat ccgcctcaag ggccggtcag cattattagc
      541 ccacagacgc cgcaatttct taaagacctc tatttgtcac tggtggaagt gtcgatgctg
      601 gagaatgttt gccgcgatga tgtaaaccgc ttccgcgccc gctttggact agccccggtg
      661 aagagaattt tcgccagatg gttgaattcg cccgatcttg tagtcctggc ttttcctgag
      721 tggtacgctc aaagacagaa agactggcct gagaatctct gttatacagg ctttcctctt
      781 tttgatagaa agcaggaaca gcgcctcagt gatgccaccc gtacctttat cgatgccgga
      841 gcggcgcctg tggtgttcac ggccgggtcg gccatggctc attctcgcga ttattttagc
      901 catgctctta aatgtgtgag agcgagaaat attcgcgcca tttttgtcac ccgctatccg
      961 gaagtcttgc cgaggcactt gcccgacaat gttttgcatg caagctatga accctttgac
     1021 catctcttcg ccgaagcgtc cgtggtttcc catcacggtg gtatcggcac cagtgcccag
     1081 tgtttgcggg ctgctgtgcc tcagcttgtc tgtcctttcg cccatgatca atttgataat
     1141 gcgcgcaggt tggaagccct tggtgtagcc agagaaatac gagttgagaa accgaagcaa
     1201 tgggccggaa agttgggcga acttctcgat gatcctgagg tgaaagtttc ttgccgcaag
     1261 gtgaaagagc ttatgacagt ttgcccagac ccggccgcaa gagcggcaga gatcatcgaa
     1321 ggattggcta aaaagctgcg taaatgaagg gttcggtggg cagcgaaagt ctgaagatga
     1381 cgtagtggaa gaaggcgccg aagataatcc ccatagttag agccagccat gggtttttct
     1441 tgaggatgaa gttttccaga gtagcgggtt gtttatcttc tgccatttct agttcttcct
     1501 ttctctgaat tctttcaggc gcttagctaa ttctgtggcc atggctttgc cgccttccac
     1561 gctcaaatga tcgaaatcca catagtgctc gggtgcgagc ttgtcgttca aggaccaggt
     1621 ttgaaaacct tgccgggtga agttttccag ccagagttct tttatatgcc ggtattcttt
     1681 tttctctctt tcgttcaact cgtctatgaa gagaggattg atggccgtta cacagacgat
     1741 caggttgttt ctggcgttgg gcggcacgga agcctgtgtg cagcctgaaa gtctgcgcca
     1801 gaagaattgc tcatattcgc tgggcggacc tttttcgtag agagcggtga tgccggccat
     1861 aaaacgtttg acaatattgg tgccgtgttc aagcttttcg ttgtcgcttg cctcgaatgg
     1921 cggcctgtct tcgaatgggt cttgaaagag acggcgcggt aaaagagagc gaaattcagt
     1981 ggttctgtgg aagtagttgc ctgcgacgct gtaagtgaac tgcgtccaca attcttccac
     2041 gcccaggtag tgattcaact tggctcgcaa ctccaggttc tcttgatgcc cttcatcttt
     2101 gaagagagtc cattccctgt gctgccggct gaaggtttct cgtgcggcgt agggcaggag
     2161 catgcccctt tcacgggcat cgaaatacat atagtgcagg ggagtggtgc cggatggctc
     2221 gaagaccgtg gagggcatgg catcggtgag caggaagacg ttttgtcctt cgctcaagag
     2281 tttttcgtaa acccagtagc cggcttcgaa aaccttggtg cctggtaggg ctagattgat
     2341 gacgacaaaa tcgtctccta gctcttgctg gagatatttt gtccagcagt gctcggaact
     2401 ttgcccttct ccgtagaata ctgagtctcc gccgatgatt aagaggggcc tgcctggttt
     2461 gtcggccagc agcagacgag cctgggccag catctgattg taggtcaggt agaagccgcc
     2521 gttggggttg atgtattcat ggaagcgcac gaagttggaa aggggcaggg gcttgcctgt
     2581 gatataggca ccggcagcgg tgctcagtaa aaagccgagc atggtgccga gcagaaaata
     2641 tttggaataa cttttcttct cagtcaccac ttactccctt gaattgaaag agcatcagga
     2701 acatggacaa ggctttgtcc aggggataga agaagggcag ccagccgaag accacaaaga
     2761 ggaaggtggt gagcctggcg gtgagtggat atttgtcata gagtttccag agcgggctta
     2821 gccaggggag cttgcgatag accgtgttta tggccaggcc cagaccgtgg tacagtcccc
     2881 agaataggaa gttccaactg gcgccgtgcc agagaccaca gagggcaaag gctagtatgc
     2941 cgcttagaat tcttctagcc agaccatgct ggctaccgcc gaggggaata tagatgtagt
     3001 cccgaatcca gaaactgagc gacatgtgcc agcgctgcca gaaatcacga atactggtgg
     3061 caccgtaggg tccgttgaaa ttagggggta gtttgatgcc catcatcatg gccagaccga
     3121 gggcaatatc ggtgtagcct gaaaaatcgt agtaaatacg accggccagg gccagaagaa
     3181 acagccagcg ctcgggcagg tcgacagcga agtagaaggc ttgcttttcc agaatatatt
     3241 gattgatgtt gtcggcgatc atgagtttct tgaagaagcc cgccgccagt ctgaccatgc
     3301 cggccgctac gtctctggaa cttacggcgg aaagcccctg atagagcgaa ggaatgaagt
     3361 cttgatagcg cttgatggga ccggctacca gggacgggaa gaagatggcg aaggccagaa
     3421 agtcttgcgg tttctttatg ggttccctgc ctcttctcac ttcgtacagg taatgaatga
     3481 attcaaaggc aaagaaactg atggccaggg gtggtgtcgc cggtagtgcg gtaaagagcg
     3541 aggcggcaaa atgctccgcc aggggtttgt tgacggatgc cagaacatcc aggagcagga
     3601 agcggctgta tttgtaagtg agaagtgctg ccgtcactat tatgattgtg cttaggcaga
     3661 gccagccgcc gccctttttg aactttgacg ctgtcagtgc ggttacatag gtaagcaggg
     3721 ccagtacggc aatcggtgcc acaccggcgg caccggcaaa atggctatga aagacggcgc
     3781 agaagataag cagggctgag aggcgcaact tgaccataag agaggagctc aagtttttgc
     3841 acaggcccag gcaccagtaa agcggaagaa agagagctac tgaaattaag aaccagtagg
     3901 tgcagaagat cactagcagt aacttccgtc aaggcttctc aagcttatct caccggcgga
     3961 aagttctacc ctggtgccgt ccgttttctc aatgagaagc gccccgctct cggcaatatc
     4021 cacggcccga ccacttatat tgcgggcgct gtcgccggtg ggacttacga ccatcacttc
     4081 tttgccgagg gtaaccgagt agtgcttcca gctatcaatg atggcctgcg acttgccttc
     4141 cagcagggaa aagtattgtc tttcaaggaa gcgacagatc tccgccacca gtagactcgg
     4201 ctccagtggc ctgccggcta aattctccag ccaatcgatg cggtttttca gatcgtccgg
     4261 gatatttcct tcatcgaggc gcaaattgat accgatgcct ataaccagcg cctgacccgg
     4321 catttcagcg aggatgccgc ctaccttttt gccgccggca gtaaggtcgt tgacccactt
     4381 gagacccagt tggataccgc agagcgcctc tactgcctgg gccacggcta ccccggtggc
     4441 cagggtgatt aagggcaggc gggtgagggg aatttccgga cgcaagagta tggacatggc
     4501 aataccggca tcgggcggcg agatccaggt gcgcccctgt cgaccgcgtc cggcggtttg
     4561 ctgtctggct acgatcaggc tgccggccgg ggcgccctct cttgccagta cagcggcccg
     4621 gctgttggtg ctgtcgattg tgtcgtgcaa ttcacagacg ccacgaccga ttagctcggt
     4681 ggtaagggcg tcttctatgt ttttgcggtc tagaatgtct aaatgtagtg tcatctcgct
     4741 cattttagtg aagcgaggct agcttttcca gcttttattc aggacttatg ggcaacttcc
     4801 gtggcgattt gcttgggacc ttccagcagt ttgtggatgg ggcacttgtt ggcgatggat
     4861 ttgagatttt ccacctgctc cggcgtaagg tcacccgtca actcgatgct tctcaagata
     4921 cgggtctgct tgctgccgtc ttcggctact acaggctctt ccaacacctc tacggtgatg
     4981 ctcttaagat cccattcttt gcgtttggca tacatctgta ctgtgattgc cgtacaggca
     5041 cccagggagc cgagcagcag ttcatgggga ttgggggcgg attcgccgcc gccgaactct
     5101 ttgggtgcgt cggaggcaaa ggcatggctg ccggcgctta tctcttgtct gtagccttct
     5161 ccaactttag ctttgactgc tactttcgcc attttctata tctccttgcc ccagggctct
     5221 atcaggagca agatattatc atgtctgtat ctactgtatc tgccccgggc cctataagtg
     5281 gtagggcgtc ccttaatgcg ggagccattc cggtctacta gttcttgttg tatatggctt
     5341 tagctctgga ctttatgtct atctgagatc tttttgagat ccgctgcgat ttggaaattt
     5401 tattttcaag cctctatggt tccgctatcc aacctggtat cacaagtgta gcggttgtat
     5461 atccatccga acttttgact aaatgtcgat cctcatggct actagcttgg acccccttta
     5521 tttgtggcga atgagcgatc tttgccggac cttttggggt agcttgcgta gcatttttgt
     5581 aagtgttacc ttgctacaca tcgcgaatcg aatcaaggag aaatttcgct ttattagctg
     5641 aatgccctga aattaaaggg atctgctcga gtctccatca cttcggttca cttatatcca
     5701 gttttgatcg cctcactgac ttcctctgca cccttgtctc aatttgagac cggtaattcg
     5761 gaagagaaat gagggagaga ttttgctggc cttttaagtg actcgaaagt tcaatcatcg
     5821 agcgatctgg tgggggaaga atggtgcaag tccatcgctg tcccgcaact gtaatagaga
     5881 gtacggaaga ccaataaaaa ccatcactct ttcagccaga ctacccacca gggcattgat
     5941 agacataacc tcttcgaggg taagaggcgg tctcaagcaa agttttcttt gtcttttgac
     6001 gccctcttgg tctcattcca ctaagggggt tttgttttgc atccacaacg tattatccca
     6061 ggagatagaa aatgacccag gtgctaactg cagtccacca tgccgacccc gctggctttg
     6121 gactgagcga tgagcgaatc ggacaatttg cactatataa agtgctgaga cgtaacggca
     6181 atgtcgttga attcgatccg tccaagatag ccgtggctat gaccaaggcc tttctggccg
     6241 tggaaggcac ccatggtcgt gagagctccc gcgtgcgcga cctggtcaac aaacttaccg
     6301 ggcaggtggt tgaaacctta atgcgccgtc tgccggaagg cggcgtgctg cacattgagc
     6361 acattcaaga ccaggtcgaa ctggccatga tgagagctgg tgagcaggaa gttgcccgtg
     6421 gctacgtgct ttatcgcgaa gcgcgcgcca aagaccgggc tgcccgtggt gcggaagtgc
     6481 cgcaaccggt gcaagccggt cctaatgtca aacgcaccga cggcaccatg gtgccgctct
     6541 cccagttggg tttgaatgct atcgtcaaag atgcctgcgc cggtctaggt gccgtagtgg
     6601 agccggagag catctacacc gccaccattg aaagccttta tgaaggtgtg caggaaaccg
     6661 aagtggtgcg ctcggccatc atgagtgccc gggctctcat cgagaccgat cctgcctaca
     6721 gctttgccac cgccagattg ctcttgcacc agatgagact ggaagttctc ggtgaaggcg
     6781 tcagtcagga aggcatgaaa gagcgctatg cgacttattt cccagagtat atagccaagg
     6841 gtatcaaggt cggtttgctt gacccgcgcc tggctacctt cgacctcgag aagatcagca
     6901 atgccctctg cgctgagcgt gatctcaagt tccagtatct tggtttacag actctctacg
     6961 acagatatct cttgcaccag gaaggtatca ttttcgaatt gccgcaggct ttctggatgc
     7021 gcgtggctat gggactttcc atcaatgaaa tcgatagaga gacccgtgca atcgaattct
     7081 ataatctgct ttccaacttt gatttcgttt cctctacccc cacactcttt aattccggta
     7141 ctcacagacc gcaactttct tcctgcttcc tcaccacagt agatgatgac ctctcctcca
     7201 tctacgaagc cgtgagagag aacgctttgc tttccaagtt cagcggcggc ctcggtaatg
     7261 actggacacc ggttcgcgct cttggtgctc atatctccgg caccaatggc aagagccagg
     7321 g
//
LOCUS       DAOQNZ010000013         7223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3930_length_7223_cov_3.875698, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000013 DAOQNZ010000000
VERSION     DAOQNZ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3930_length_7223_cov_3.875698"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PKW73_00450"
     CDS             complement(<1..1100)
                     /locus_tag="PKW73_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71776.1"
                     /translation="MNQGKTSKDKGLLDRVFSDIFERELRACAEETYEGEKYVNGATF
                     TAKAAPEGTDKSAEATPKLETDSRDKPVRTSKQTAETEKKKITKTVEIVVGQAAKNAD
                     ADKKKKATKTVEIVMEPSSKQTNHPSPETTVEKTVEMPVAQETAFEENKPEGKLEGKP
                     EATQQEKSEEKADGKPESKADENGADDSQEFFARPFQEVPSDFISDYNLGEDSETGDY
                     DSSEGYETGYSDWSYETPRPWLSIDEAASVLGRSSRAVERSILGRWGNRLPEGWSARL
                     VKIDGADQDQWRVIPPRGFRLRANRKSATVATEIDGETSKPTQEPEAQAQTDSEPQAK
                     AETSMKEGSDSQERTNRDLTDRKNQAQSETTGR"
     gene            complement(1425..2036)
                     /locus_tag="PKW73_00455"
     CDS             complement(1425..2036)
                     /locus_tag="PKW73_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNA71777.1"
                     /translation="MLQQKISYKPYAKHDFSHLSGLTGISDKTLEIHLALYAGYVTNT
                     NTLNEKVIELTEAGKSGTPEYAELKRRYGFEYNGMRLHEYYFGNLKPGGSELKEGSEI
                     GKAIIDTYGSIETWKNDFFKVGGMRGVGWAILFQDPETKVLSNHWITLHEEGNIAGFK
                     PILVMDVWEHAFLLDYKPAERPKYIEAFYSNVDWAKVESRLIK"
     gene            complement(2313..4112)
                     /locus_tag="PKW73_00460"
     CDS             complement(2313..4112)
                     /locus_tag="PKW73_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71778.1"
                     /translation="MTAGDPAHSANHQTAQPSGSAPLRLGDLLTSAGLLKAEELRQAM
                     LIAKSQSLPVGRVIIMSGFMSEQHLQAAVQCQSLLKDGLLNTENAVAALRTLYRENCT
                     LDEALHKLGIQQGGPTNKLGELLIEAGLVTKEQLEASLVQGQTSGLPLGRVLVVTGIL
                     TEQMLASVLNAQILIRDKRVDREQAISSLKAARDRQVPLEQTLAETGIAMPNAETVRL
                     GELLVMAKLLEEAQLMQIVEVGLVKEQPIGQVLVESGVVTENTLQQALGVQNYVSSGK
                     LRKSLSGQVLELVHNQNLTIEAAIERVQPERQQISNNLPLYQFLQLAGVIGPKDIEDA
                     LKAGSRNAQLMGQMLLLTGAVDQNLLQIAMKCSDLMAQGILKAEQAVIALGICWKTKA
                     TLEEAFRQLGWSPAIIAAAFPSHPTPMAATFPPHSQLSDQAPGLAQLQAQQQAQQQAQ
                     SQTLPAAAGTTSQTSASQAQTMPPTKPIGPGSSGSHFQTSSHQSPLAQALNKGRNADS
                     SSGGLPAVSARESNRESMKRKSDATSASLPALATPGQPALNLLQELETTGDNEPTGDV
                     TGEHDAVGQSPGGAGQLRKKKLSDLIPQIKNQG"
     gene            4754..6034
                     /locus_tag="PKW73_00465"
     CDS             4754..6034
                     /locus_tag="PKW73_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71779.1"
                     /translation="MVPSICLTGMCLWLVICHVHYHSYWMGTIYRVQTTDFNLLHHTL
                     PPVLSDMILANRDDLIQRTLDCSFGLFGMVITDPSGENVLYKTEKIYRRESWLKRVQP
                     ESLLKESEPYDLLTDPPQLEPLFAHKSPRSTQAVQVGTTKRGVKVLGRLYYLRSAPPS
                     FAEDLSNFLLNGFWELSGSKRGYCYITCSCIGFTLAILLLVYLRRRGIELKQKELEHM
                     QRELDIRKKALESLGSELATQKARKVWLEREADQAYKRAIGLKQSLERLKESLGGAMQ
                     DNFAPADGSQTVVSTGANGVTGGSGISGTNGRISQIKIRPPVAPPGSILEEIESLLPA
                     LSENAKNLRSQADVLQDYCSALEDRQVEMRKIVENAFSRTTAITGATNSSGVAPPGLA
                     STHVSPAAPAPSTLPASKAPVQETGPDFIDMSPR"
     gene            6211..>7223
                     /locus_tag="PKW73_00470"
     CDS             6211..>7223
                     /locus_tag="PKW73_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNA71780.1"
                     /translation="MKQVSGKKIALTLSAVQDASSEPVYRRGVQYYRRKKVLKYTEDE
                     GVSNIEALVIGSEPQPYKVTVTIDSPEVFKAECSCPYFEEVCKHSVAVLLTHIARHNP
                     GIRFDLMDGPERKEAPVEKAPKAKELLDDPREEGVVPDARDFQLGLLVLEKPLAMIVG
                     DVPPQPQGKVNILKVPPESLNFFPAESRIWRLAKYLTSLPQTTKGTAGGHRIPRGEEG
                     TVIGHLSLCAKLLNTTNGLEVTFSDQPLKPRAVLSETESGELLLKLEGITEEGEAVEH
                     PVFFMGQSSWILSNNVFYPIDLSAIHRSFQYFDSYGFMTVKLEVVPKFLSVDLPVLLK
                     RMPV"
BASE COUNT         1392 a         1946 c         1944 g         1941 t
ORIGIN      
        1 cggcctgtgg tctcgctttg tgcctggttc ttgcggtctg taaggtcccg gtttgtcctt
       61 tcctggctgt cgctgccctc tttcatgctg gtctcagctt ttgcctgggg ctccgagtcg
      121 gtctgggctt gtgcctccgg ctcttgcgtg ggtttgcttg tctcgccatc gatttcagtg
      181 gcgacggtgg cggatttgcg gtttgctctg aggcggaatc cgcgtggggg gattactctc
      241 cactgatctt gatcggcacc atcgattttg actaagcggg cgctccaccc ttccggcagt
      301 cggttacccc agcggcccag tatggaacgc tccacggctc ggctggaacg accaagcacg
      361 ctggctgctt cgtctatgga gagccagggg cgtggagttt cgtaggacca gtcgctgtaa
      421 ccggtttcat aaccttcgct actgtcgtag tcgccggttt ccgagtcttc acccaggttg
      481 tagtcgctta tgaagtcgct tggtacttcc tggaaggggc gggcgaaaaa ctcttgtgaa
      541 tcgtcggcgc cgttctcgtc tgctttgctt tccggcttac cgtcagcctt ttcttctgat
      601 ttttcctgct gagttgcttc cggcttgcct tcaagctttc cttccggctt gttttcttca
      661 aaagcggttt cttgtgcgac cggcatttct accgtctttt ccactgtcgt ttcggggctg
      721 ggatggttag tttgcttgga acttggctcc atgactatct ccaccgtctt ggtggccttc
      781 ttctttttgt cggcatcagc atttttggct gcctgcccga caactatttc tacggtcttg
      841 gttatcttct tcttttcagt ctcggcggtt tgtttggaag tccgtaccgg cttatcccgg
      901 ctgtcagtct ctagtttggg tgtcgcttcc gcgcttttat cggtgccttc cggcgccgcc
      961 tttgccgtga aagtggcacc gttgacgtat ttttcgcctt cataggtctc ttcggcacag
     1021 gctctcagct ctctttcgaa tatgtcgcta aagacccgat cgagaagccc tttatccttt
     1081 gaggttttgc cctgattcat aaccgctttc ttctttgttt tcagacaatc gactggatgc
     1141 catcgtgcag tttaatatgg tagcgttttc aagtggaata gtcttgaatt gttgcttagc
     1201 tacagggcaa ttaccgtctc ctggcatcaa ttatttctca tggaagggtc cgacctttgc
     1261 tgtttgccgg gtcgcagacc tctttggcag tagggctgat tgcccatggc tacctgttgt
     1321 gcctgctgcc caagccgccc gccgccgcag ccgcttgagg cgcgatagcg caaaaaaaaa
     1381 gaaggcgggg gtgaccgcct tctttccaga tttagaaccg tttcttactt gatcagtctc
     1441 gattcaactt tggcccagtc tacgtttgag tagaaggctt cgatgtactt gggtctttct
     1501 gccggtttgt aatcgagcaa gaaagcgtgt tcccatacgt ccataaccag aatcggcttg
     1561 aatccggcga tattgccttc ttcatgcagg gtgatccagt ggttggaaag cacttttgtt
     1621 tcgggatcct ggaagaggat agcccagccc acgccacgca tgccgccgac tttgaagaaa
     1681 tcgttcttcc aggtttcgat ggaaccatag gtgtcgatga tggctttgcc aatctcagag
     1741 ccttctttca gctcgctacc gcccggtttg agattgccga aatagtattc gtgcagtctc
     1801 atgccgttgt attcaaaacc atagcgacgc ttcagttcag cgtattcagg agtaccgctt
     1861 ttgccggcct cggtgagttc gattaccttt tcattcaagg tgttggtgtt tgtgacgtaa
     1921 ccggcataga gagccaggtg gatctcaagg gtcttgtctg aaataccggt caaaccgctc
     1981 agatggctga agtcatgttt ggcataaggt ttgtacgaga ttttttgttg aagcattgaa
     2041 aactcctgtg caacacagca tttgtgggga cagaaccgct gcggcttgca ccgagcgact
     2101 ccactaaatt cctgattgcg gattagttgc taatttttac cataattccc ccgccagtct
     2161 cgtcatttgt ggcttaaatt cgcgggtttt tagggattct caggctttgg tcttggcaat
     2221 ggcgcgctcg tttgccatgc ctgcggcaaa gaccgcgcag tcggccctgg caagatttgc
     2281 tctcgtgctg ctgctcctga caggcttgga tctcagccct ggttcttaat ttgcggaatc
     2341 aggtcggaga gttttttctt gcgcagttgg ccggcccctc cggggctctg cccgactgcg
     2401 tcatgctcac ctgtgacgtc tccggttggt tcgttgtcgc ccgtggtttc aagttcttgc
     2461 aagaggttca gggccggttg accgggggtc gcaagagcgg gcagagatgc tgaagtggca
     2521 tcggatttgc gtttcatgct ctcccggttg ctctccctgg ccgatacggc cggtagaccg
     2581 ccgctggagg aatcggcgtt gcgccccttg ttgagagcct gggccagggg tgattgatgg
     2641 cttgaggtct ggaaatggga gccggagctg cccggtccga tgggcttggt aggcggcatg
     2701 gtctgagcct gggaagctga agtctgggaa gtagttccgg cggcagccgg tagtgtttgg
     2761 ctctgggctt gctgctgtgc ttgctgttgg gcctgcagtt gagccagacc gggagcctga
     2821 tctgaaagtt gtgaatgggg ggggaaggtg gcggccatgg gtgtcgggtg gctggggaag
     2881 gcggcggcga tgatggccgg gctccagccc aactggcgaa atgcctcttc cagtgtggct
     2941 tttgttttcc agcaaatgcc aagggcaatg acggcctgtt cggctttcag tatgccctga
     3001 gccattaaat cactgcattt catggctatt tgcaagaggt tttgatctac tgcaccagtg
     3061 agcaagagca tctgtcccat aagctgtgca ttgcgtgaac cggctttgag agcgtcttcg
     3121 atgtcctttg gtccgatcac gccggccagt tgaagaaact gatagagggg cagattgttg
     3181 ctaatctgct ggcgctccgg ctgcacacgc tcgatggccg cttcaatggt gagattttga
     3241 ttgtgcacca gctccaaaac ctggcctgaa aggctcttgc gcagtttgcc ggaagagaca
     3301 taattttgca cgccgagggc ttgctgcagc gtgttttcag tcaccacgcc gctttccaca
     3361 agtacctgac cgatgggttg ctctttcacc agtccgactt ccacaatctg catcaattgg
     3421 gcttcttcca gaagcttggc catcacaaga agttcgccca gccgcactgt ttccgcgttc
     3481 ggcatggcaa tgccggtttc cgccagggtt tgctccaggg gcacttgccg gtcgcgggca
     3541 gccttaaggc tggaaatcgc ttgctctcta tctacgcgct tgtctcgtat caaaatctga
     3601 gcgttgagca ccgatgccag catttgctcg gtcaaaatac cggtcaccac cagcactctt
     3661 cccaggggca gcccgcttgt ctgaccttgc acaagcgagg cctcaagctg ctccttcgta
     3721 accaaacctg cctcgatgag cagttctccc agtttattgg tcgggccgcc ttgctgaata
     3781 ccaagcttgt gcagggcttc gtccagggtg cagttttccc ggtagagagt tctcagggct
     3841 gccacggcat tctcggtgtt cagaagaccg tcttttagca aagactgaca ctgcacggca
     3901 gcctgcaagt gctgctcgct catgaagccc gacattatga tcactcgacc caccggtagc
     3961 gactggcttt tggctataag cattgcctgg cgcagttctt ccgcttttag caagccggca
     4021 ctggtgagca agtcgcccag tctgagaggc gcagaccctg agggctgtgc cgtttgatgg
     4081 tttgccgaat gtgctgggtc gcctgccgtc attaagtgat cctgatgttg agtcttgatt
     4141 ttatcgcaac tagtttcaaa gcaacacttt catttttccc tgttttctct tgactatggc
     4201 ggattttcca ggatttatgg cggattgttt ttgtgggaag gtaaataggc gccgtttatc
     4261 ttctcggccg gacggcggcg gcactaccgg cagtgtcatg ccgaaaagcc gggtggttag
     4321 acccggcttc tggagaacac actagcttta tgctctagtt tttctatgct ctattttgtt
     4381 gcagccaccg cttgggtgga attcccgtat gggtttttag tagacagaac gtgtgtctta
     4441 tcgatggggc tctaactgat tcctccgcta gatcatcgtc tactcttact gaataccccc
     4501 tcctcagtga cttataactt ctcctcagtc tgcagcgaaa gaaaatttct ttttggttgt
     4561 cttccttacc ggcatccggc agatttgttg cctgccatac acataagacc atcttgcggg
     4621 gcagtttaat ggggccgcct gggaaaagcc gtatgtacga aatgcatata tgggtgtaga
     4681 cttcggctgt tcagtggtca atttatatag gtaatagatg gacaaattcc ttctatccct
     4741 gaaaaatcgt gtcttggttc cgtctatatg cctgacaggc atgtgtttgt ggctggtcat
     4801 ctgccatgtg cactatcaca gctactggat gggaaccata tatcgggtgc agaccaccga
     4861 ttttaatctt ttgcatcaca ccctgccgcc ggtgctgtct gacatgatcc tggcaaaccg
     4921 tgatgattta atccagcgca ccttagattg ctccttcggc ttgttcggca tggtcatcac
     4981 tgatccatcc ggtgagaatg tcctctataa aaccgagaag atctatcgcc gcgagtcatg
     5041 gctgaagcgg gtacagcccg aaagtctctt gaaagagtct gagccctacg accttctcac
     5101 cgatccaccc cagctagagc ctctctttgc gcataagtcg ccgcgctcca cccaggccgt
     5161 gcaggtcgga accaccaagc gcggagtcaa agtgttgggt cgtctctatt atttacgcag
     5221 cgctcccccc tctttcgcag aagacctgag caacttcctc ttgaacggat tctgggaatt
     5281 gtcgggctcc aagcgcggat attgctacat tacttgctct tgtataggct ttaccctggc
     5341 tattttgctt ctggtttact tgcgccggcg gggcattgag ctcaaacaga aagagctcga
     5401 gcacatgcag cgcgagctgg atattcgcaa gaaggctctg gagagcctgg gcagcgagct
     5461 tgccacgcag aaagcgcgga aggtctggct ggaacgggag gccgaccagg cttataagcg
     5521 ggctatcggg ctcaaacagt cgcttgagcg cttgaaagaa tcgctgggcg gcgctatgca
     5581 ggacaatttt gccccggcag acggctctca gaccgtggtt tccaccggtg caaacggagt
     5641 taccggaggt tctggaattt ctggtactaa tgggcgcatc tcgcaaatta aaattcgtcc
     5701 gccggtggct ccacccggct ccattctgga agagattgag agtcttttgc cggctctttc
     5761 ggaaaacgcc aaaaacctgc gcagccaggc tgacgtgctg caagattatt gcagcgccct
     5821 tgaggaccgc caggtggaga tgcgcaaaat tgtcgaaaac gccttttcgc gcaccacggc
     5881 cattaccggt gcaactaatt ccagcggcgt ggcgccgcct ggtcttgctt ccacccacgt
     5941 aagtccggcc gcccccgccc cttctacgtt acccgcttct aaggcgccgg tgcaggaaac
     6001 gggacccgat tttattgaca tgagcccacg ttagtctggc ttaatcttgt tcatcaaacc
     6061 tttagcaagt catgctaagc tattggctga ctggctgtta attgagcagg aagccactcg
     6121 cgaaccgccc acaagcggtt gcctgttgtt attgacagtt gtcttgtcga accccataga
     6181 gagtagtttg gctctcaatc tcgctattaa atgaagcaag tatccggtaa gaagatcgcc
     6241 ttaaccctct ccgctgttca agacgcaagc agcgagccgg tttatcgtcg aggcgtgcaa
     6301 tattatcggc gcaagaaagt tctcaagtac accgaagacg aaggtgtatc caatatagaa
     6361 gccctggtca taggctccga accccagcct tacaaggtga ccgtcaccat agactctccg
     6421 gaagtgttca aagccgaatg ctcctgcccc tatttcgaag aagtttgcaa acactcagta
     6481 gctgttttgc tcactcatat cgcccgtcat aatcccggca ttcgctttga tctgatggac
     6541 ggacccgagc gcaaagaagc gccggtagag aaagcaccca aagccaaaga attactcgat
     6601 gatccccggg aagaaggggt cgtacctgat gcccgcgatt tccaactcgg tcttctggtt
     6661 ttagaaaagc ccctggccat gattgtgggc gatgtgccgc cgcagcctca gggcaaggtc
     6721 aacatcttaa aagtgccgcc ggagtctctc aattttttcc cggctgagtc tcgcatctgg
     6781 cgcctggcca agtacctcac ctcgctgccc cagaccacca agggcaccgc cggtggtcac
     6841 cgcattccac gcggcgaaga aggcaccgtg atcggtcact tgagcctctg cgccaaactt
     6901 ttgaatacca ccaacggtct ggaagtaaca ttctcagacc agcccctcaa accgcgggcg
     6961 gtgctctccg aaaccgagag cggcgagctt ctgctcaagc tggaaggcat caccgaagaa
     7021 ggcgaagcgg ttgaacaccc ggtcttcttc atggggcaga gcagttggat cctttccaac
     7081 aatgttttct atcccataga tttgagcgcc attcaccgct ccttccagta cttcgattcc
     7141 tacggtttca tgaccgtcaa gctggaagtc gtacccaagt ttctttccgt cgacctgccc
     7201 gtgcttctga agcgcatgcc ggt
//
LOCUS       DAOQNZ010000014         7211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_3938_length_7211_cov_7.950252, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000014 DAOQNZ010000000
VERSION     DAOQNZ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3938_length_7211_cov_7.950252"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1599..2468
                     /locus_tag="PKW73_00475"
     CDS             1599..2468
                     /locus_tag="PKW73_00475"
                     /inference="COORDINATES: protein motif:HMM:NF025669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNA71781.1"
                     /translation="MAAEKYRHIVREFLFVRRFPDLSPSAIKPGSHLVAAFRCSAGHT
                     YEKAIYRRTQKNKPEGCPYCAGKRSDAPTLFSLFPYLLKRWHPEKNVVMDKVSGEMVE
                     IEPDTVLADSRKKYWFTCPSGHSYQASPKEITENADQCQKCAILPNCVDNVRPELTEQ
                     WDSKKNGELTPWNTAAGSSTLVSWRCKAGPDHQWQAKVYKRALEKNGCPYCANRRLSI
                     TNTLEAKYPELAKLMLPEDNGGLEASEVIASMSSAFNWRCGCSRTYRRLLRHMVESGT
                     GCNECRAQKGHSS"
     gene            2664..3197
                     /locus_tag="PKW73_00480"
     CDS             2664..3197
                     /locus_tag="PKW73_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71782.1"
                     /translation="MTNQNTNRPPPTSWRRRLAQDPPPSSNVADSYFERLMRYIPADI
                     VAGYVAIDGILKDGGNSPLWLTWSVFISLLVLTPLYVCYLKTVPPGMAISKTFHWVAS
                     MLAFSAWVFALGGPFAVTFDWYRPVYGSVLLILTTLTLPVLESIFYGGSPPTPPAVPT
                     LPTAQPQPPEDPPGGAE"
     gene            3220..3768
                     /locus_tag="PKW73_00485"
     CDS             3220..3768
                     /locus_tag="PKW73_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71783.1"
                     /translation="MRYRSLAILFALVLGALTLGGCSVAPSVAVHPLEVGRPSVVSTD
                     ASARGIILLPKAGGQGMYVCAEPSPDVAMAAVAKMIAEVKTQNPAIDVKTQLEFQTAV
                     VQLAQRSQTLQLLREGMYRACEAGINQNLSAEQVMKLHEMAMQAALKLAESELAKNKA
                     DLAKSLRDPRVRELFQQMTEPK"
     gene            3827..4690
                     /locus_tag="PKW73_00490"
     CDS             3827..4690
                     /locus_tag="PKW73_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71784.1"
                     /translation="MTKSKKAVKAEQPVLGLVIEASGAAALLITRRRRLFRAGGSVFV
                     GELLRKDGKTWLKASLAVPSIERNGWDLAHETAARYKKEFGQSPYIEPDRTGGRYDAL
                     AQSGKARDPMMPSLDGAPGRSAWDPNAMIESLMPKIPHVGLAGNMGIPGIGGRGNGGF
                     GGFGIPDMTMGMGMGQAPGGFGQSQIGPAGQGQDVMALLQQAGLGGSEEAEISVPALR
                     AVPGFEGLSDNLAKMLEVEASQLLLSQMSGVSPGAHNKDSLVAIGKLPECSRVLKSFL
                     SGVESSEVEAD"
     gene            4944..7106
                     /locus_tag="PKW73_00495"
     CDS             4944..7106
                     /locus_tag="PKW73_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71785.1"
                     /translation="MATKFVDLQNGNDTNNGTTFALRKKTLSSAASVAAAGDVIRVMG
                     NPSTNSGTATWTKGSPLVTLSAAINQAIYNDGAWMAAANVTATPNTTAPTPKQGSNAS
                     KLACAAGFTTGKIAYFPTAGLLDLSAYQQVSFWIQCSTALASGALTLDLCSDTSGDVV
                     VHSFPINVALNANLWTAVTVDNAAALSASIQSVRLSAVSSMASKNVLIDNIFAAKAKG
                     AADCLTLNTLISPDNATWYPVQSVNGTTVYVDAQQATAAAAAPGYRGATGSSTFYMLQ
                     PTVVSIGTGNTVYDQVFSANGASGNPIVISGGWNTTDMSTQDGWTTIDRRDWAASGIN
                     LSGTTGHITVEKFNFARAARPLGLVSTARGYSFNNSGCAGTGSFSSMPTRAVTIAGSN
                     FINCSGTTAILNIPATANYQADNKNWSITNTKVWGASVGGIKLAPFTSSPFATIAGCD
                     CSGNTGIGIDVQSPCYFRDNTAEGNTTFGINFQSLEDAVAYNLTARGNATGEVQLSNA
                     EVEIYGLDTNTVGGSAIPQIYMPNNVGGRAVIYNWTQYTGGAPAAQLTRLGSPATNQA
                     SGNTVNAQKEAGNAANNSIYTDFGTATTTGVVGQPGSGIAWKLTPNAKALADSPLKVN
                     VGKVACPANVPTTIKYWAKHSAASGISSRLRVPGGRYAGVGSPGTDVVSATVSGTAFA
                     QYSVTFTPTENCVVDVFFETWGSTTQNATVSGPVAISQ"
BASE COUNT         1696 a         1842 c         2027 g         1646 t
ORIGIN      
        1 gatcagttat gagtgaccaa taaacctaat gacacagttt gattgacaga tccgcctcgg
       61 ttctgagcag tctccagaga ccaatcttga tctggttagc taggcgcggc aatgttaagc
      121 aaaatcttga gcttttgaac ctggccgatt gtctctccgg tgccaccacg agtatcaaag
      181 tgtctcctga aagttctcgg ttcttgtcca tatatttcgt tacgggatga agtactgtaa
      241 acccgctgat agtggtgctt tagagtagca atcatggaaa aactgatcaa agtataaatc
      301 ctagcgcgaa cacttggctg cgctctgtcc tctgaaacca ttgattccag atggcttcaa
      361 gctttttagc tgtcagtatc tagcgcgctg gctgtttgag ttgctctcat tattggaaaa
      421 aactagtctc ggcctgtgga ttttgccgca tcggatcgac tctattctag gtatctattt
      481 ttcttcccgt tccaggaaat ctctccgcca accttactaa ctgttctcct cccactgacc
      541 gaacaccact ccccagaagg ggttgggcgg atctgcttta aggaatgagg ttcttagtct
      601 gcttctgttg actagttgga aattcatttt gcgaccgaaa atccttgtat gaggaaggca
      661 aaaagttgtc ccggttcttg acgacaggcg gcagaagtaa cttactatgg gttcattgga
      721 aaaggccaag gaattcggtg agctagcttc gaaaggttct gataagccat gggtaaaacg
      781 gcccaccgtt gctggtatca cctgttggca ggctgttcta aatttcgaga gggacaatcg
      841 acttggccaa gaagaaattg aaggatttgc caagagcatc cggtcaaata cgcacactgg
      901 gccaataccc actaagcaaa agaccgcctg gaccatctct aagacaaagc cacccggctc
      961 ttgcgcttga gttgattgac aagggacctt acacggcaga tgacctggtt gctggctcta
     1021 acagagtcgt cctctggcgt cacggtcgat gtgggcaaga gatatgggtt tctgtctccg
     1081 tgcggtgccg ggcctgggat gaaggttccg cgttccaagc ctgctacaag tgtgctggcc
     1141 ggcaagaacc tttacagagg caggcacagg tcataccaaa atggctacaa aagcagtgca
     1201 taccggacaa gaagcgcttg accaagatca aggacatatc gccctggtca cagacttact
     1261 ggcaatacaa ctgtccgaag ggctcaggaa aagtcttttc ctcccgcata gtagacaggg
     1321 tgaatccaga agatgaaact gagaagcaag gttgcccctg tgccagatgt tataccggag
     1381 agcgccggaa tatctacacg cagagccaaa caagctttga tctcaaggca atgtatctca
     1441 ccaccgggga gaatctaggg tttgacccaa agaggctgcc tctaacatcg agagtgcgct
     1501 ggcggtgtga taaggattca agccatctct tttcaagaag cctccttgaa ctagaagaga
     1561 agggatgccc aaagtgccgg acggttgaga aagattcctt ggcggcagag aagtacagac
     1621 atattgtgcg agagttcttg tttgtgcgcc gttttccaga tctttctccg tccgctatta
     1681 agccagggag ccatctggtg gcagccttta gatgttctgc cgggcacaca tatgagaagg
     1741 ccatctatcg tcgcacccaa aagaacaagc cggaaggttg cccgtattgt gcaggcaaaa
     1801 ggtccgatgc accgaccttg tttagcctct ttccgtatct gcttaagcgc tggcatcctg
     1861 aaaagaatgt ggtgatggat aaggtcagcg gggagatggt tgagatagag cctgatactg
     1921 ttctggctga ttcccgcaag aaatactggt tcacttgtcc gtccggtcat agttaccagg
     1981 catcgccgaa agaaataaca gagaatgctg accagtgcca gaagtgcgct attttgccaa
     2041 actgcgtgga caacgtcaga ccggagttaa ctgagcagtg ggatagtaag aaaaatggtg
     2101 agctcacacc atggaatacg gctgcaggct cgtccacgtt ggtatcgtgg cggtgcaaag
     2161 ccggaccaga ccatcagtgg caggcgaagg tctacaaaag agcactcgaa aagaatggat
     2221 gcccatattg cgcaaaccgg cggttgtcga taaccaacac gctggaggct aaatatcctg
     2281 aactcgctaa gctgatgctt ccagaggaca acggtgggct tgaggcatcg gaggttatcg
     2341 cctcgatgtc cagcgccttc aactggcgct gtggctgttc aagaacctac agaaggctgc
     2401 ttcgacacat ggtggagagc ggtactgggt gcaatgaatg ccgggcgcaa aaaggacatt
     2461 cgtcctagtc ctttgggaga aaatgcccga aacgaaggag agtgcaccct gtcctgcaaa
     2521 ggtcgtggta gaagtgcttt gggtgctttg aagccctttc gagtgcagtg aattctatgc
     2581 aaaattagac ctaatagtga tggttgaagt agggactaat ttgccctgct cctgcctcag
     2641 tcatatcaat tggagtcaaa tgaatgacaa accaaaatac aaataggccg ccgccgacat
     2701 catggcgacg gcgtcttgcc caggatccgc caccaagcag caatgtggcc gactcctact
     2761 tcgagcgtct gatgcggtat attccagccg acatagtggc gggatacgtc gcaatagacg
     2821 gcattttaaa agacggggga aacagtccgc tgtggctgac ctggtctgtt tttatctcac
     2881 tgttggtgct gacaccgctt tacgtctgct atttaaagac ggtgccgcca ggaatggcca
     2941 tcagcaagac ctttcactgg gtggcctcga tgcttgcctt cagcgcctgg gtttttgccc
     3001 tgggtggacc gtttgctgtc acttttgatt ggtacagacc ggtatatggt tctgtgctgc
     3061 tcattcttac tacattgact ctgccggtac tggagagcat cttctatggc ggtagtccac
     3121 caacacctcc ggctgttccc accttgccga ctgcgcagcc acagccgccg gaagatccgc
     3181 cgggcggagc tgaataggac accaaataag gaatagagaa tgagatatag atctctagca
     3241 atattatttg cactggttct cggtgcgctt accctgggag gctgttctgt tgctccctca
     3301 gttgccgtgc atcctcttga ggtaggtaga ccatcggtgg taagcactga tgcttccgcc
     3361 agaggcatca tcctactgcc caaggcgggt ggtcagggca tgtatgtttg tgcggagccc
     3421 tctcccgatg tagccatggc cgcagtggca aaaatgattg ccgaggtgaa gactcagaat
     3481 ccggcaattg atgtgaagac gcagcttgaa tttcaaacgg ctgtggtgca gcttgcccag
     3541 cggtcgcaga ccttgcagct tctcagagag ggcatgtata gagcctgtga ggctgggata
     3601 aatcagaatc tgagcgccga acaggtaatg aagctgcacg aaatggctat gcaggcagcg
     3661 ctgaagctgg cggagtctga gctggctaag aacaaggctg atctggccaa gagcttgcga
     3721 gacccaaggg tgcgcgagct atttcagcag atgactgagc cgaaatagcg gcgctgtttt
     3781 gagcgctgct tcagttgcac aattttgaaa tcaggaaatg aacaatatga caaaatcgaa
     3841 gaaagcagtt aaggctgagc agccggtgct tggcctcgtg atagaggcga gtggggctgc
     3901 ggctctactc attaccagac ggcgcaggct gtttcgtgcc ggtggttcag tctttgtggg
     3961 agagctattg cgaaaggacg gcaagacatg gctcaaggcc tcgcttgcag tgcccagtat
     4021 cgagcgcaac ggttgggatc tggctcatga gacggcagct cgctacaaga aggagttcgg
     4081 gcagagccct tatatagagc cggaccgcac cggtggtcgg tatgacgctc tcgcgcagag
     4141 cggcaaagcc agagacccca tgatgccgtc cctggatggc gctcccggca gatctgcctg
     4201 ggaccccaat gccatgattg agagtctgat gccgaagatt cctcacgtag gtcttgccgg
     4261 caatatggga attccgggca ttggtggtcg gggtaacgga ggtttcggtg ggtttggaat
     4321 ccctgacatg accatgggca tgggcatggg gcaggctcca ggtgggtttg gacagtccca
     4381 gattggtcct gccggtcagg gtcaggatgt gatggcgctg cttcaacagg ctggcctggg
     4441 cggcagtgag gaagctgaga tttcagttcc tgctctcagg gccgtacctg gctttgaagg
     4501 gctgagcgat aacctggcaa aaatgcttga ggtggaagcg tcgcagctgc tgctctcgca
     4561 gatgtcgggg gtgtcgcctg gtgcgcataa caaggatagt ttggtggcta tcggcaagct
     4621 gccggagtgc tctcgggtgc tcaaatcatt cctttcaggg gtggaaagtt cggaagtcga
     4681 ggccgactga agaatcattt gctttggcac cgtaatccaa agctgattct gcaaatagtc
     4741 agctttgatc ttgcggtgcc agtttttctg ccgcgcatgt tgggggctgt ctcaagcatg
     4801 cagtcaggca gttgtttggc gcattgacat cggcattgcc ggtgcgctct gtgctgcccg
     4861 caagagttag tccggatcaa atagctgacg ccatgcttca aagccggtgt cggcgcaaaa
     4921 cctcaatcaa aaaggtaaat caaatggcta caaaattcgt agatctccag aacggcaatg
     4981 ataccaataa cggtaccaca ttcgccttgc gcaagaaaac cctctcgtcg gcagcttctg
     5041 tggcagcggc tggcgatgtc atccgcgtca tgggcaaccc atcgacaaat tcaggaactg
     5101 ctacctggac caaaggctca ccgctggtga ccttgtcggc ggccatcaac caggctatct
     5161 acaatgatgg cgcttggatg gctgctgcaa acgttaccgc tacaccaaac acaactgcac
     5221 cgactccgaa gcaaggcagc aatgcatcca agctggcttg cgcagcgggc tttaccacag
     5281 gcaagatcgc ctacttccct actgccggtc tcttggactt gagtgcttat cagcaggtct
     5341 ccttctggat tcagtgcagc acagcgcttg cttccggcgc actgaccctg gacctttgca
     5401 gtgataccag tggtgatgtc gtggttcact ccttcccgat caacgtcgca ctaaatgcca
     5461 acttgtggac ggcggtgacc gtagacaatg ctgcggccct gagtgcatcg attcagagcg
     5521 tgcgcttgag cgccgtgtct tcgatggcga gcaagaacgt gttaatcgac aacatctttg
     5581 cggcaaaggc aaagggcgca gcggactgtc tgaccctgaa cacgctgatt tcgcctgata
     5641 acgctacttg gtacccggta cagagcgtca acggcaccac cgtgtatgtg gatgcccagc
     5701 aggcaaccgc tgcggctgct gctcctggct atcgcggcgc taccggctcc agcaccttct
     5761 acatgttgca gccgactgta gtttctatcg gcacaggtaa cacagtctat gaccaggtgt
     5821 tctctgccaa tggtgccagc ggcaacccga tcgtaatctc cggtggttgg aacaccactg
     5881 atatgtccac ccaggatggc tggaccacga tcgaccggcg tgactgggcg gcttccggca
     5941 tcaacttgag tggtactacc ggtcatatta cggtcgagaa gttcaacttt gctcgtgctg
     6001 cccgtcctct tggactggtg tctacggctc gcggttacag cttcaacaac agcggttgtg
     6061 ccggtaccgg ctcgttctca agcatgccca ccagggcggt gaccattgcc ggctcgaact
     6121 tcataaactg ctcaggcacg acggcgatat tgaacatccc agccactgcc aactaccagg
     6181 ctgacaacaa gaactggagc atcaccaata ccaaagtgtg gggtgcgtcc gtgggcggca
     6241 ttaagcttgc gccgtttacc tcctcgcctt tcgcgaccat tgctggttgc gattgctcgg
     6301 gcaacaccgg cattggcatc gatgtgcagt cgccttgcta cttccgggac aacacggcag
     6361 aaggaaacac gactttcggc attaacttcc agtccctgga ggatgccgtt gcttacaacc
     6421 tgacggctcg cggcaatgcc accggtgaag tgcagcttag caacgctgaa gtggaaatct
     6481 atggactcga caccaacacg gttgggggct ctgctattcc gcagatttat atgcccaaca
     6541 acgtgggtgg ccgtgccgtg atttataact ggactcagta taccggtggt gctcctgcgg
     6601 cgcaactgac caggctggga agtcctgcga ccaatcaggc gtccggtaac accgtgaacg
     6661 ctcagaaaga ggctgggaac gctgccaaca actctatcta caccgacttt ggtacggcga
     6721 cgaccactgg tgtggttgga cagccgggtt caggcattgc ctggaagcta actcctaatg
     6781 ccaaagcttt ggcagacagc ccgctcaagg tgaatgtcgg caaggtggct tgtccggcta
     6841 atgtaccgac cacgattaag tactgggcga agcacagtgc agcaagcggc attagctcgc
     6901 gtctgcgggt gcctggcggc cgctatgccg gtgtgggttc ccctggtacc gatgtggttt
     6961 cggcaacggt aagcggcaca gcgtttgcgc agtactcggt gacctttacg ccgacagaga
     7021 actgcgtagt tgacgtgttc ttcgagacat ggggctctac cacccagaat gcgacggtca
     7081 gcggacctgt tgcaatcagc cagtaaacac aagaaggaga agcaagaatg gctacaaaaa
     7141 ccttcgatct aggcaacttc gcctatcagg cagcaccctt catttggggc tcgctgcaag
     7201 acttaagcgg c
//
LOCUS       DAOQNZ010000015         7129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4009_length_7129_cov_4.147017, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000015 DAOQNZ010000000
VERSION     DAOQNZ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4009_length_7129_cov_4.147017"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..370)
                     /locus_tag="PKW73_00500"
     CDS             complement(65..370)
                     /locus_tag="PKW73_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71786.1"
                     /translation="MFKETLRLMHALAFGKASMTFFLGKNVVAGIACALTAPTVSEAL
                     WRVLPWFGVLAGDLLWYALPWLWRRLKTVPQEPLPHWRGKLGGTYCGRKLELVRVHS"
     gene            complement(545..1474)
                     /locus_tag="PKW73_00505"
     CDS             complement(545..1474)
                     /locus_tag="PKW73_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71787.1"
                     /translation="MAAIPFCLYAAGLSLEEIVDCAIGLDFETLVRPQEKLSSVLKTH
                     YQAGRRKGRLPGKGIYSMENFAKWLETRLKGEWPRRELFWTMATDDNGAQVLFTENGM
                     FKRIQGEAFQQIAAPARPESAICASCTVPGFFIPINLPLPTGGQLQLYDGALSFEGTR
                     PLSVVDFFAFKQGTRLVFCDVGAERNNYDRLYNSIWRIICGGRCLPSYKAKTGEKNFG
                     MPVVEILPTVTAVRSFEFSAPASRKWKAILEGFAATTQALTRSGLLSLEETMQARGLL
                     QEVEHLEKACQEERLDFSVKLRALLILRGVLKS"
     gene            complement(1665..2033)
                     /locus_tag="PKW73_00510"
     CDS             complement(1665..2033)
                     /locus_tag="PKW73_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71788.1"
                     /translation="MFNSLQKSKTTIITRDALGRHRRVFTTDTPVNEVTVEYFGVLLA
                     LPGADQFSYHPSRQRIYLDFEPEANVDAAADRIKPTLLALNIKVEEQKPISRQLIQLI
                     QRLNHGASTCRSKFFGDKET"
     gene            complement(2156..4270)
                     /locus_tag="PKW73_00515"
     CDS             complement(2156..4270)
                     /locus_tag="PKW73_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA71789.1"
                     /translation="MFPKKTNGQKNPRRGPNMNWFLYAGLASIVILAIWWVMPSSSSG
                     DNLLKFHSETALSKIQQVIREKPQTIKEITFVRIRGESFPGKVLLDSTTEEGGIWSPI
                     AGDEEYKRISATADSAHVAINNKIADPDQKSSLLISMLISVAPWIIFIAVAGYFFTKM
                     APAASGNQNKKANLIRLDQVSEKVLLKDVAGIEDEKRTLEELILDLKHPELITSLKGK
                     IPRAVLLKGPPGVGKTFVLQAIAGETGLPILSGAGSDFVEMYVGVGAARIRDAFAQAR
                     QLRNETGNWVILFIDEFTSIGQNRADGNNNEHKQTVDALLVELNGAGDTNDRILFVAA
                     TNQPEALDAAVTRSGRLGDLIIEIGKPDKQGREAILKVKLSNMPVADDIDIEKIAQEM
                     SGMTGADIDTLVKKRAPTQAKRRLLKGVSPELLLKPEGFKVSDHIKTEDLKITMDDLW
                     IALMEMVLGNINETKGKRIDEQVKRMIAYHETGHLTVAMRKLLQNTGSWNGQYGDEIT
                     DISILGPNGTGGFVRTNPRHDMQTARNLKSRLAVAMGGNAAERLFLGDTTGGCQNDVE
                     VANKIIKVMLLQINMSQYHNHQEGGQQLRLPAISVKQMGGSKYLGGMVTDVPQYGMSD
                     YSASQVDRLIGVYLNEAEQEASAYLEEERDWVEFFVPKLIEKERMRFAEIQSLWNEFH
                     GDKDLSKSFAFVYDWDENHKRG"
     gene            complement(4321..5061)
                     /locus_tag="PKW73_00520"
     CDS             complement(4321..5061)
                     /locus_tag="PKW73_00520"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA71790.1"
                     /translation="MLLPTLGILPLMDTSLPRWTSRTESEKGSRKIPWASTVIILTCL
                     VAQLLVSGTSADSELEHYRLYGVNAGPALIALKSADPFQIGATLLAMLRATFVHTDWI
                     HLVQNMALVMAFAPAIELILGAARMLFLYLLAGILAMIVTITVNPEQLLLGASAACFG
                     LAGAYLHLRPQGRIAGLLLVGLQSFVLPIPFVLKATWAILLMVLAQVLFKLGEPALVA
                     HLTGLSLGYLAARWLKQSANKTTTPASL"
     gene            5613..6470
                     /locus_tag="PKW73_00525"
     CDS             5613..6470
                     /locus_tag="PKW73_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71791.1"
                     /translation="MIESSYKSACSGLEGLIEAAKKASDRQGTTNGKNKSSADLNWAV
                     ECWRTAALRSAYLLAANHDTTIFCQVNLGRALGDAGHYEEAVSLLEMLLTRLEERGSA
                     VGPRAHLFLGPVFVGIIYEVLARAHSGLGNELRSCRYWLKAASSSQEYRQLHNSSTIY
                     CYHQAARSLARLKLREEALLAFKLVYDGNNKLFGDHLLTAFAARDLAVCLNRLKRFAE
                     ARQYWVRAQVLMEAFLPEQHDLRKKVAAGRFWTDRKVRDMRKERKRLRRLERAALPAA
                     ASVASAESA"
     gene            6566..>7129
                     /locus_tag="PKW73_00530"
     CDS             6566..>7129
                     /locus_tag="PKW73_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71792.1"
                     /translation="MKQFWKKADLLRSDLLGSLSDTVSGPSRQAVIFDVLQIFGGIAL
                     FLIFATLSRYLYWWMDLALLAGTAVFTISTPLECRYRLLLTTAFLLSSFYFFLGQPLL
                     APLGWLVGGLSLTLIVYEWKYPSGRTLVWLFLLGTAVSALIMTASALFLLPYVHPYTT
                     MLVGWMPAVWVVLTVLLAAIASLRQRSG"
BASE COUNT         1410 a         1913 c         2084 g         1722 t
ORIGIN      
        1 ttgcaatcga gcggaagaga aaagctatag cagtcctttc tcttccgtct cgtttgtcga
       61 aggcttagct gtgcacccga acaagttcga gtttgcgccc acagtatgtt cctcccagtt
      121 tgccgcgcca atgcggcaga ggctcttgcg gcacagtctt gagacggcgc cagagccagg
      181 gaagcgcata ccagagcagg tcgccggcca gtacaccgaa ccagggcagc actcgccaga
      241 gggcttcgct gactgtcggt gctgtgaggg cacaggctat gcctgccact acgttctttc
      301 ccagaaagaa agtcatggaa gccttaccaa aggcaagggc atgcatcagt ctgagtgttt
      361 ctttgaacat agctgcctct ttgtttgctt gatatacaaa aagagtgcag tctccacatc
      421 tgtcgaattg aagacagatg taagagccct gcccaataag aatcctggcc tcgatgtcac
      481 ctattctcat tgggcagggc tcaggcagaa gaagcgggct gcctcctctg cctgaacctt
      541 tctttcagga cttcaaaacc cctcgcagga tgagcaacgc tctcagtttg actgagaagt
      601 ccagccgctc ttcctggcag gctttctcca ggtgctctac ctcttgcaag agtcctctgg
      661 cttgcattgt ttcttccaga gaaagcaagc cgcttctggt cagggcctgg gtagtggcgg
      721 cgaagccttc cagaatcgcc ttccacttcc tggaagcggg agccgaaaac tcgaaggagc
      781 gcaccgcagt caccgtcggc aaaatttcca caaccggcat gccgaagttc ttttcacccg
      841 tcttggcttt gtagcttggc aggcagcgcc ctccgcagat gatgcgccag atggaattgt
      901 agaggcgatc atagttgttg cgctcggcgc ctacatcgca gaagaccagc ctggtgcctt
      961 gcttaaaggc aaagaagtct accaccgaga gcggcctggt tccttcgaaa ctaagggcgc
     1021 cgtcgtagag ttgcagttga cctcccgtgg gcaggggcag attgatcggg atgaagaagc
     1081 cgggcacggt gcaggaggcg cagatggcgc tttcgggacg ggccggtgcg gctatttgct
     1141 ggaacgcttc tccctgtatc cgcttgaaca tgccgttttc ggtgaagagg acctgggcgc
     1201 cgttgtcatc ggtagccatg gtccagaaca gctcgcgccg gggccattct cctttcagtc
     1261 tggtttcaag ccatttggcg aagttctcca tgctgtaaat acccttgccc ggcagccgcc
     1321 ctttgcggcg accggcctgg taatgggtct tgagcacgga agagagtttc tcctgcggtc
     1381 gcaccagagt ctcgaaatcg agaccgatgg cgcagtctac aatctcttcc aggctcaagc
     1441 cggcggcgta gaggcagaag gggatggccg ccactgatac cgcccatact ggccactttg
     1501 agcttgccca gccagtttaa ggccagcagc atgcccagat tagccagcat ggcgcggcag
     1561 ccgcccgagc cggctaccag atgcagtttt tcgtctgcct gctgtgagat ttgagcagac
     1621 atgtgggata tcctttctct tttggcggca aggtccgacc ctgatcaggt ctccttgtcg
     1681 ccgaaaaatt tcgagcgaca ggtgcttgct ccatgattta accgctggat cagctggatc
     1741 agctgtcgcg aaattggttt ctgctcttcc accttgatgt tcaaggccaa gagcgtaggt
     1801 ttgatgcgat ccgccgccgc atccacattg gcctccggtt cgaagtcgag atagatacgc
     1861 tggcggctcg ggtggtagga aaattggtcc gctcccggca aggccagaag cacgccaaaa
     1921 tattccaccg tcacctcgtt caccggcgta tccgttgtga agacgcgacg atgtcgcccc
     1981 agggcatccc tggtaatgat tgtggttttg ctcttctgga gtgagttgaa caaggttgtt
     2041 cctttctctg ctcttgaggc tcgattgtga agagaggagg ggcagtcgtc cctcctctta
     2101 ctcttgtctt ttgctgtttc agtcctcagt tgttcggact tcagctgttc aggccttagc
     2161 cgcgcttgtg gttctcgtcc cagtcgtaga cgaaggcgaa gctcttcgag agatccttgt
     2221 cgccgtggaa ttcattccag aggctctgaa tctcggcaaa gcgcatgcgc tccttctcga
     2281 tcagcttggg aacgaagaat tccacccagt cgcgctcttc ctccaggtag gcgctggctt
     2341 cctgctcggc ttcgttgagg tagacgccga tcaggcgatc cacctgcgag gccgaatagt
     2401 cggacatgcc gtactggggt acgtcggtga ccatgccgcc caggtatttg gaaccgccca
     2461 tctgcttgac gctgatggcc ggcaaccgga gctgctgtcc gccttcctgg tggttgtgat
     2521 actgcgacat attgatttgc aacaacatca ctttgatgat tttgttggca acctccacgt
     2581 cgttctggca accaccggtg gtatcgccga ggaagaggcg ctcggcagcg ttgccgccca
     2641 tggccacagc caggcgcgac ttcagattgc gggcggtctg catgtcgtga cgcggattag
     2701 tgcgcacgaa accgcccgta ccgttgggac cgagtatgct gatatcggtg atttcgtcac
     2761 cgtactgacc gttccaggag cctgtgttct gaagaagctt acgcatggcc acggtcagat
     2821 gaccggtttc gtggtatgca atcatcctct tcacttgctc atcgatgcgc ttgcccttgg
     2881 tctcattgat gttgcccaga accatctcca tgagagcgat ccagagatcg tccatggtga
     2941 ttttcagatc ttccgtcttg atatggtcgc tcaccttgaa gccttccggc ttgagcaaaa
     3001 gctcgggcga aacgcccttg agcaaacgac gcttggcctg ggtgggggcg cgcttcttga
     3061 ccagggtgtc gatgtcggcg ccggtcatgc ccgacatttc ctgggcgatt ttctcgatgt
     3121 cgatatcatc ggccaccggc atattggaca gtttcacctt gaggatggct tcacgtccct
     3181 gtttgtcggg tttgccgatt tcgatgatca ggtcgccgag gcggccggag cgggtgacgg
     3241 cggcgtcaag cgcctccggc tggttggtgg cggcaacgaa gagaatgcgg tcgttggtgt
     3301 cgccggcacc gttcaattcc acaagcagcg cgtccaccgt ctgcttgtgt tcgttattat
     3361 tgccgtcggc gcggttctga ccgatggagg tgaattcgtc gatgaagaga atcacccagt
     3421 tgccggtttc gttgcgcaac tgacgcgcct gggcgaaggc gtcgcggatg cgggcagcgc
     3481 ccacacccac gtacatctcc acgaagtcgc tgcccgcacc ggagagaatc ggcagaccgg
     3541 tctcaccggc aatggcctgc agcacgaagg ttttgccgac gccgggcggt cccttgagga
     3601 ggacggcgcg cggaatcttg cccttgaggc tggtaatcag ttccggatgc ttgaggtcga
     3661 gaatcagctc ctccagggtg cgcttctcgt cctcgatgcc ggcaacgtct ttgagcagaa
     3721 ccttttcgct cacctgatca aggcgaatca ggttggcttt cttgttctgg ttgccgcttg
     3781 ccgccggagc catcttggtg aagaagtaac cggcgacggc aatgaagatg atccagggcg
     3841 ccaccgagat gagcatgctg ataagcagag agctcttctg gtcggggtcc gcgatcttat
     3901 tgttgatggc cacgtgcgca ctgtcggcag tggcgctgat gcgcttgtat tcttcgtcgc
     3961 cggcgatggg gctccagatg ccgccctctt ccgtggtgct gtccagaagc accttacccg
     4021 ggaagctttc cccccggatg cgcacaaagg tgatttcctt gatcgtctga ggcttctcgc
     4081 ggatcacctg ctggatcttg gagagggccg tttcgctgtg gaatttgagc aggttgtctc
     4141 cgcttgagga agagggcatc acccaccaga tggccagaat aacgatagag gccagacccg
     4201 cgtagagaaa ccagttcata ttgggtccac ggcggggatt cttttgaccg ttggttttct
     4261 taggaaacat gatgtgttcc tttcacatgt tggagaatag tctgcctgag gcgctctcct
     4321 ttagagcgac gcaggcgtgg tggtcttgtt tgccgattgc ttgagccagc gggctgccag
     4381 ataaccgagc gagagtccgg ttaaatgggc aaccagagcc ggttctccca atttgaagag
     4441 gacctgcgcc agaaccatta agaggatggc ccaggtagcc ttcaagacga agggaatcgg
     4501 caatacaaag gactgcagac ccaccagcag caggccggct atgcgtccct gggggcgcaa
     4561 atgaaggtaa gctccggcca gaccgaagca ggcggcggag gcacccagga gcagttgttc
     4621 gggattgacc gtgatggtga cgatcatggc cagtattccg gcaagaagat agaggaagag
     4681 catgcgagcc gctcccagaa tcagttctat agccggggca aaggccatga ccagggccat
     4741 gttttgaacc aggtgtatcc agtccgtgtg tacgaaggtt gctctcagca tggctaataa
     4801 ggtggctccg atctgaaagg gatcggcgct tttgagagcg ataagtgccg gaccggcatt
     4861 tactccgtaa aggcggtagt gctccagttc gctgtctgcg ctggtgccgc ttaccagaag
     4921 ctgcgcaacc aggcaggtga gaatgatgac ggtgctcgcc cacggtattt ttctggaacc
     4981 tttttcagat tccgttctag atgtccatcg gggcaagctg gtatccataa gcggcagtat
     5041 gccgagtgtt ggtaacagca tgggcgtact ttctatcagt tgaatttgtt tgctttttcc
     5101 cagcaccaaa ggctgggagg attggactga aataaggaag gtgttgccgc tggggcgatt
     5161 ttcgtgatgg atgttttgct ctttgaaaat tggctgcgtt ggaaaatgta attcgatact
     5221 tgccagaatt gctgcgtctt ttcaaggggg gcttgcggag tttggtttag ctcatggtta
     5281 cgatttggat gccgttccac ttttgcggtg ataccggggg taatgtcata aatctttcca
     5341 atttaaggag tatgagaccg ttgactgtgt agacaagagg ttgctagctt gaatttgttc
     5401 ttctctctat ttctccattc ctgcaggttg cagactcctt tcaaaatccc acccggtttt
     5461 tcgcgttgtt gatgagcacc atttcgtgct tagtagtggc attctcttct ggttggcctg
     5521 tgccgtcgat aatttgcggc ccatgggcaa cctcgaattg aggcaaaaca aaaagtctaa
     5581 aaatccaaaa atcaaaaata ggagtagcag caatgattga atcttcctac aaatcggctt
     5641 gctccggact ggagggcttg attgaagcag cgaaaaaagc aagtgatagg cagggaacaa
     5701 ccaacggcaa aaacaaaagt tccgccgact tgaactgggc tgtggagtgt tggcggactg
     5761 ccgctttgcg cagtgcctat ttactggctg ccaaccacga cacgacgatc ttttgtcagg
     5821 tcaatctggg cagagccctt ggcgatgccg gtcactatga agaagccgtc tccctgctcg
     5881 aaatgctgct cacccggctg gaggagcgcg gttcggcggt tggtccgcgt gcccatctct
     5941 ttctgggacc ggtctttgtc gggatcatat atgaagttct ggccagggcc catagcggtc
     6001 tcgggaacga actgcgctct tgccgctact ggctgaaggc ggccagttct tctcaggagt
     6061 atcgccagtt gcataacagc agcaccatat attgttatca ccaggcggcc cgctctttgg
     6121 cgcgactgaa actgagggaa gaagccctgt tagccttcaa gttggtctac gacggcaaca
     6181 acaagctctt cggggatcat ctcctgacgg cttttgccgc cagagacctg gcggtctgcc
     6241 tgaaccggct gaaacgcttt gcggaggctc gacagtattg ggtgagggct caggtgctga
     6301 tggaggcgtt cttgccggag cagcatgacc tgcgaaagaa ggtggcggcc ggtcgtttct
     6361 ggaccgaccg caaggtgcgt gatatgcgta aggaacggaa gcggcttcgt cgactggagc
     6421 gcgctgcgct cccagcggca gcctccgtgg catcggcaga gtcggcctaa acccggctgc
     6481 gaaaaacagc atactgtctc caccggcggg cataagcctg ccggggcaca gtctaaccaa
     6541 ggaacacaga atgagaaact cctggatgaa acagttttgg aagaaggcgg atcttctgcg
     6601 tagcgacctg ctcggctctc tgtcggatac tgtctccggt ccctcccgac aggcggtcat
     6661 tttcgacgtt ctgcagatct tcggtggtat cgcactcttc ttgatctttg ccaccctgag
     6721 ccggtatctg tactggtgga tggatctggc tttgcttgcg ggaacggccg tctttacgat
     6781 cagcacaccg ctggaatgca ggtatcgcct tcttctgacg acggcgttct tgctgtccag
     6841 cttttacttc ttccttggtc agccccttct ggcccctctg ggatggctgg tcggcggtct
     6901 gtctttgacc ttgatcgtgt atgaatggaa gtatccgagc ggcaggaccc ttgtctggct
     6961 cttcctgctt ggtacagccg tttctgcatt gatcatgacg gcgtctgctc ttttccttct
     7021 gccttatgtg catccataca caaccatgct ggttggctgg atgccggcgg tctgggtggt
     7081 gttgacggtg ctcctcgctg ctatcgcttc tctccggcag cgcagcggt
//
LOCUS       DAOQNZ010000016         7083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4046_length_7083_cov_3.744308, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000016 DAOQNZ010000000
VERSION     DAOQNZ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4046_length_7083_cov_3.744308"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKW73_00535"
     CDS             complement(<1..386)
                     /locus_tag="PKW73_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71793.1"
                     /translation="MVKTVKIGSMETSPSRFLIIAGPCVIESYDILLVTARHLLALSR
                     KHNFDLVFKSSFDKANRTSVNGFRGPGLTEGLKMLAEVKRELGVPVLSDVHESAQCDE
                     AAAVLDILQIPAFLCRQTDLLVAAART"
     gene            450..779
                     /locus_tag="PKW73_00540"
     CDS             450..779
                     /locus_tag="PKW73_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNA71794.1"
                     /translation="MTAVPFTSSPDGLKLRLHLQPGAKTSGFAGLRQADGPGGKTDTR
                     LVLRLTAPAREGEANQALLSFLASALKLPKSSITLETGATSRLKTVFLKGCPETLAER
                     LKQAAGI"
     gene            complement(806..1138)
                     /locus_tag="PKW73_00545"
     CDS             complement(806..1138)
                     /locus_tag="PKW73_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71795.1"
                     /translation="MEEPGSVPEDEENTAAETGTPEVKKRGRPPIVRPAGQGAKPPAA
                     ENKVVRLLEKNPLGMTLPEMAGGPRQIKRMKTLRPILADAIRLGLIMPVSQRAGHTVY
                     KLVKNMNR"
     gene            1313..2614
                     /locus_tag="PKW73_00550"
     CDS             1313..2614
                     /locus_tag="PKW73_00550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA71796.1"
                     /translation="MTDFATAIQNRYRITGELGSGTMGNVYKAVQLATNRPVAIKVLA
                     SDLLRDSKSLKRLKREAKALSVLNHTGIVCVYEFDTHSAPVPFMVMEFVPGRSLKAIL
                     KSRQLKLEETVNLMLQAAKALSHAHYKGIIHRDVKPANMLIDEERMELKIVDFGIARP
                     ASNLTQEQLTMQGEVFGSPLYMSPEQCQGIEIDARSDIYSLGCVLFECLTGHPPYRGV
                     SAFDTMTMHLNSVVPKLEDELDLGPYRALATIVEKTLCKKPADRFASMSDLATSLAIL
                     KETVVINAEPQKAEPGKRMPSSKVTVPSTSRQRGSKTHGIKLLLLVMVGLAAVLASIY
                     VGLSWKKESISKSAFTPKLPLKQGTDAPTEGNHRSPMQEYEEQRVPLNGPPENMENPQ
                     STGSQMQEGGVYQVDIPVNGSGATQAPVKDQAPAVDSGKQQ"
     gene            2776..4443
                     /locus_tag="PKW73_00555"
     CDS             2776..4443
                     /locus_tag="PKW73_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNA71797.1"
                     /translation="MKSVSIGSFVFMLHSHLPYYRKAGMWPFGEESVYECMAETYIPL
                     LNAIAELYNEGLNANLTIGLTPVLCEQLADEHIKDGFETYLQARIKAARQDEGRYTNR
                     GSDPNPELHHLSRFYLNWFLSIEKDFKEKWNRDILAGFKKYQDLGAIEITTSAATHCF
                     SPLLEEDVSLQAQFQTGVDNYRKHFGRAPKGFWLPECAYRPSKEERAGIEKFLHEAGI
                     EYFFTESFVIKGGETAEVRRVVGPYGSVQYLSSATTTNTGLDTYEAFWLKDYQVAVMG
                     RHEEAGYKVWSADHGYPGDGNYREFHKKDDQSGLHYWRLTSKSTDLGAKEIYNPEAAE
                     SRMKENSDHYVGFVQDCLTEHLKATGKPGLIMVSFDTELFGHWWFEGVSWLKEVIKKL
                     KTYTAVKMTRASDYLAENPPEKTIELKESSWGSGGHFQVWQNSETDWMWPHIHRAEKQ
                     MAEVADLPTVNHDKLITRAARQLAREMLLLSSSDWPFLITTGQAKDYATERFNDHRER
                     FESIYKMIKGGNVDEAALQDIEDKDNCFEEIDLHHFSRKSLPKTITV"
     gene            4500..4802
                     /locus_tag="PKW73_00560"
     CDS             4500..4802
                     /locus_tag="PKW73_00560"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNA71798.1"
                     /translation="MTNPSSVRLPVLVNLQTSERFTIENQSITLGRDPDCQLVLPEDG
                     FASANHCRIFWDQGRLWLEDLMSSNGTSLNEQLITRPSQLSPGDVIKVGHTRFRIE"
     gene            complement(4809..6059)
                     /locus_tag="PKW73_00565"
     CDS             complement(4809..6059)
                     /locus_tag="PKW73_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71799.1"
                     /translation="MQEENKEQEAGAEGQTKPRRVPKTLLDAEVNLKPQRRVAKTMLE
                     SSLPTQAKRRVAETMLEISKPDLNAVDAAEACAEPAGGNVLPSASRKERVAKTMLEMS
                     LPIMESEAAPRERKKTRKIAKTLAVQDIRSLIEAEALNQSAQLEAAGASPAPTPVKKE
                     KQSVAKTMLDHDVLFQAVSQSFVLKESRMQEVIRQKASQPLVIIEPIECKKKALPCKW
                     RWPESDSGKVERFRYCDKCQTPVYNFDGLSLEQAESVIFQRENIKKPTLYKRADGKFM
                     TRDCPVEVKRRKDRGLMIAGAGLFVIIFLTILFNLPPAPKPAVTQEDVPVKHKGGSST
                     KGASTSTEKKGSLVRTSDGFHYDADAPASSSPTTVAVPVTPPADTAAPPAPLSPRDES
                     GEFWQFDKNEGAESQGQYPNASRP"
     gene            6242..6730
                     /locus_tag="PKW73_00570"
     CDS             6242..6730
                     /locus_tag="PKW73_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71800.1"
                     /translation="MARAGKSKRNCHGGSSIAEFGPALGIILICFFFPMIDLLAMGVS
                     YGLCMVLNHNQAHEAALLPNADATNAGGIVKKGIPDQWLDGMGRFVKLSGYPSTNVSY
                     RNGVADASNNQDKFVTVSTTLTCNPFLPIPVPVLNVPGLNGPMTFTVTSECQLENPDY
                     AP"
     gene            6773..>7083
                     /locus_tag="PKW73_00575"
     CDS             6773..>7083
                     /locus_tag="PKW73_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71801.1"
                     /translation="MIEAVSAAMIIIPVTLVLLDLLVLVIANSMNDSAAKNAARAAAN
                     QSDAASAFNAANKSLESFKPSSIIPELKIQTFNYPNAQDIVTCKTRMKVVLPVPFPGY
                     H"
BASE COUNT         1748 a         1970 c         1789 g         1576 t
ORIGIN      
        1 gtgcgagctg cggccacgag caaatcggtc tgccggcaga gaaaggcggg aatctgcaaa
       61 atatccagca cggctgccgc ttcatcgcac tgggcacttt cgtgcacatc ggaaagcacc
      121 ggcacgccca gttctctttt cacttcggcc agcatcttca gaccctcggt cagaccgggt
      181 ccgcgaaagc cgttcacgga agtacgattg gctttatcaa aggaggattt gaaaaccaga
      241 tcaaagttgt gcttgcggga aagggccaag agatgcctgg cagtgacaag caaaatatcg
      301 tagctttcaa tcacgcaggg accggcgatg atcaaaaacc tgctgggact ggtctccatt
      361 gacccgattt tgactgtctt taccattttg cacactctca aaactcgtgt ataccaaaag
      421 ctatcgctaa gggttttaat tgtatcagta tgacggcagt cccttttaca tccagcccgg
      481 acgggctcaa actaaggctt cacctgcaac cgggggcgaa aacatccggc tttgccggtc
      541 tgcgccaggc cgatggaccg ggcggtaaga ccgataccag gctggttctg cgcctgacgg
      601 cgccggccag agagggagaa gccaaccagg ctttgctctc cttcctggct tcagcactga
      661 agttgcctaa atcatctata accttggaaa ccggcgccac cagccgcctc aaaacagtct
      721 tcctgaaggg ctgcccggaa actctggcgg agcgactgaa gcaggcggcc ggcatctaaa
      781 cagcggctga atcagttatg agagttcagc ggttcatgtt cttgactaac ttgtagaccg
      841 tgtgtccggc ccgctgtgag acaggcatga tcaatcccag gcggatggca tcggccagta
      901 ttggacgcaa ggttttcata cgcttgattt gccggggccc accggccatc tccggcaggg
      961 tcataccaag cggattcttc tccagcaacc tgacaacctt gttttcagca gccggcggct
     1021 tcgccccctg gccggccggt ctgacaatgg gaggacgtcc gcgttttttt acttccggtg
     1081 taccggtctc agccgcggta ttctcttcgt cctccgggac agagcccggt tcttccatat
     1141 tcacaatccc acctatacca gagaatcttt accctcgaga cttaccgtgt agctctaccc
     1201 accgcaaaaa tgctcaagag caagcgccat gcgagtgcta tcatacgcgc cagagaccaa
     1261 gctgaaaaga tcaccggatt agtcaggggc ggcagtaagg atatcaagta cagtgaccga
     1321 tttcgcaacg gccatccaga atcgatatcg cattaccggt gaactgggct caggaaccat
     1381 gggtaatgtc tacaaagccg tacagctagc caccaaccga ccggtggcca tcaaagtgct
     1441 ggcctcagat ctactgcgcg actccaaatc attgaagcgg ctcaaacgag aagccaaggc
     1501 gctttccgtc ctcaatcata ctggcatcgt ctgcgtctat gaatttgaca cccactcggc
     1561 ccccgtgcct ttcatggtta tggaattcgt gccggggcgt tctctgaagg caattctcaa
     1621 atccagacag ttaaagctgg aagagaccgt caatctcatg ctgcaagcgg cgaaggcctt
     1681 gagtcatgct cactataaag gcatcattca ccgggatgta aaaccagcca atatgctcat
     1741 cgatgaagag cgcatggaac tgaagattgt ggacttcggc attgccagac cggccagcaa
     1801 tcttacccag gaacagctga ccatgcaggg ggaagtattc ggcagcccgc tctatatgag
     1861 cccggagcag tgccagggca tcgagataga tgcccgctcg gacatctatt ccctgggttg
     1921 tgtcttgttt gaatgcctga ccggccaccc gccctaccga ggtgtctccg ccttcgatac
     1981 catgaccatg cacctcaaca gtgtcgtgcc gaaacttgaa gatgagcttg atctgggacc
     2041 ctaccgggcc cttgccacaa ttgtggaaaa gactctctgc aagaagcctg ccgatcgctt
     2101 tgccagcatg agcgatctgg ccaccagcct ggcaattttg aaagagacag tagttatcaa
     2161 tgccgaaccg caaaaggcag agcccggcaa gagaatgcca agcagtaagg tgacggttcc
     2221 aagtacgtcc cggcagcgcg gcagcaaaac ccacggcatc aagcttttac ttctggtaat
     2281 ggtgggactg gcggctgtgc tggcctctat ttacgtcggc ttaagttgga agaaggaatc
     2341 tatttccaaa agtgccttca cgcccaaact tcctctcaaa cagggtacgg atgcccctac
     2401 tgaaggaaac catcgctccc cgatgcaaga atacgaggaa caaagggtgc ccttgaacgg
     2461 tcctcctgaa aacatggaaa accctcagtc cacgggatct caaatgcagg aaggcggggt
     2521 ctatcaggtg gacattcccg tcaatggttc aggcgccacc caagcaccgg ttaaagacca
     2581 ggcacctgcc gtcgacagcg gcaaacagca ataaagaata gttacaggtt atcaccggct
     2641 cacccgatcc tgcacctact agttgatata ttcctctgta ttctttgcaa gcgttcgcca
     2701 cttgcaaaca agaggcaaca acacgcaagt caagcggcgg tctattcctg agacggtcgc
     2761 agcaattgaa gaggaatgaa gtcggtgagc attggctcct ttgtatttat gttgcacagc
     2821 catttgcctt actaccggaa ggccggcatg tggcccttcg gagaagagag cgtttacgaa
     2881 tgcatggcgg agacttacat ccctcttctc aacgccatcg ctgaactcta caacgagggt
     2941 ctgaacgcca atctcactat cggcttaact cccgtgctct gcgagcaact ggccgacgaa
     3001 catatcaaag acggcttcga gacttatctg caagcgcgca tcaaggcggc ccgccaggat
     3061 gaaggccgct ataccaaccg cggcagcgat cccaatcccg agttacatca cttgtcgcgt
     3121 ttctatctga actggttcct ctccattgaa aaagatttca aagagaaatg gaatcgcgat
     3181 attctggccg ggttcaagaa gtatcaggac ctcggtgcca tagaaatcac aacttcggca
     3241 gccacccact gtttctcacc ccttctggaa gaagatgttt cactgcaagc gcaatttcag
     3301 accggcgtag acaactatcg aaaacacttc ggccgggcgc ctaaaggttt ctggctgccc
     3361 gaatgtgcct accgtccatc caaagaagag agagccggca tcgagaagtt tctgcatgaa
     3421 gccggtatcg aatacttttt cacagaaagc tttgtcatca aaggcggtga aactgcagaa
     3481 gtgagacgag tggtcggacc ttacggctcg gtgcagtatc tttccagtgc caccaccact
     3541 aataccggtc ttgataccta tgaagccttc tggttaaaag actatcaagt ggccgtgatg
     3601 ggtcgccacg aagaagccgg ttataaggtc tggtctgccg atcacggcta tcccggcgac
     3661 ggtaattacc gggaatttca caagaaagac gaccagtccg gtttgcatta ctggcgcctc
     3721 acctccaagt caaccgatct gggcgccaag gaaatctaca atccggaagc ggcggagagt
     3781 cgcatgaagg aaaattccga ccactatgta gggtttgtgc aagattgcct gaccgaacat
     3841 ttgaaagcca ccggaaaacc aggactgatc atggtttctt tcgacacgga actcttcgga
     3901 cactggtggt ttgaaggtgt gagctggttg aaggaagtaa ttaaaaagct gaagacctat
     3961 acggctgtga aaatgaccag agctagcgac tacctggccg aaaatcctcc ggaaaaaacc
     4021 atcgagctaa aggagtcatc atggggctcc ggcggacatt ttcaagtctg gcagaacagc
     4081 gaaaccgact ggatgtggcc gcacattcat agagccgaaa agcaaatggc ggaagtggcg
     4141 gatctgccca cggtcaatca cgacaaattg atcacgagag cagccagaca gttggcccgc
     4201 gaaatgttac ttctctcgtc atccgactgg cccttcctca taaccaccgg gcaggccaaa
     4261 gactatgcca cggaaagatt caatgaccac cgtgaacgct ttgaaagcat atataagatg
     4321 atcaaaggcg gcaatgtgga tgaagcagcc ctgcaggaca tcgaagataa agacaattgc
     4381 ttcgaagaaa tcgacctgca ccatttttcc aggaaaagtc tacctaaaac tatcactgtc
     4441 taggtataaa ctagtctagt tcagtttaga gcggccaatc tcagagttcg gaataatcca
     4501 tgaccaatcc atcttcagta agactgcctg tcctggtcaa tctccagacc agcgagcgct
     4561 tcaccatcga gaatcagtcc atcaccctgg gccgtgaccc ggactgccaa ctggtcttgc
     4621 ccgaagacgg cttcgcctct gccaatcact gccgcatctt ctgggatcag ggccggctct
     4681 ggttggaaga cctgatgagc agtaacggca catccttgaa cgaacaactg atcacgcgcc
     4741 ccagccaact ttcacccggt gatgtgatta aggtcggtca caccagattt cgcatcgaat
     4801 aaagaccgtc agggacgaga ggcattgggg tattgcccct gagactcagc cccttcattc
     4861 ttgtcaaact gccagaattc gccgctttca tcccgaggag aaagaggtgc gggcggggcg
     4921 gcggtatcag caggcggagt gacaggcact gccacggtag tcgggctaga acttgccggc
     4981 gcatcagcgt catagtgaaa accatcggag gtgcgcacca gtgaaccttt tttttcggta
     5041 cttgtgctcg cgcccttggt cgaggagccg cctttgtgtt tcaccggcac atcttcctgc
     5101 gtcacagccg gcttcggcgc cggcggcaag ttgaacagga tagtcaaaaa gatgattaca
     5161 aacaaaccgg caccggcaat catgagaccg cgatccttgc gccgcttcac ttccacggga
     5221 caatcccggg tcataaactt gccgtcggct cgcttatata gagtgggttt ctttatattc
     5281 tcgcgctgga atatgaccga ctcggcctgc tccagactga gcccgtcgaa gttatagacc
     5341 ggggtctggc atttgtcgca atagcgaaaa cgttccacct taccggaatc gctctcaggc
     5401 cagcgccact tacagggcag cgccttcttc ttacattcga tgggctcgat aatgacaagc
     5461 ggttgggagg ctttttgtct gatcacttcc tgcattctcg actcttttaa aacaaaagac
     5521 tgcgagaccg cctgaaacaa gacatcgtgg tcgagcatcg tcttggcaac cgactgcttc
     5581 tctttcttga ccggcgtggg cgccgggcta gctccagccg cctcaagttg ggcgctctga
     5641 ttgagggctt ctgcttcaat gagactgcga atatcctgaa cagccagagt cttggctatt
     5701 tttcgagttt tcttacgctc ccggggggct gcttcgctct ccattatcgg caaagacatc
     5761 tcgagcatgg tcttcgccac ccgttctttg cggctggcag agggcaagac attgccaccg
     5821 gcaggctcgg cacaagcttc ggcggcatcc acggcattaa gatccggttt ggaaatttcc
     5881 agcatggttt ccgccacccg tcttttagcc tgggtgggca gggaactttc cagcatggtc
     5941 tttgctacac ggcgctgggg cttgagattg acttcggcat cgagcaaagt cttggggaca
     6001 cgcctgggct tggtctgccc ctccgcaccg gcttcctgtt ctttattctc ttcctgcatc
     6061 tctacccgtc actgaagcta tcagcactat tgtatccccg caggcaccgg ccgatgatca
     6121 aagttctaat cttgcccccc ttcttgcccc cttaagatgt attcctaaat ttccaggtgg
     6181 gcgagtataa ctacagtgtc gccgagtgtg atttaaaatc gaggctgaaa aatataccaa
     6241 tatggcaaga gctggaaaaa gcaagagaaa ttgtcacggt ggctcatcca tagcagagtt
     6301 cggaccggcc cttggcatta tccttatctg cttcttcttt cccatgattg accttctggc
     6361 catgggtgtt tcttacgggc tctgtatggt cttgaatcac aatcaagccc acgaagcagc
     6421 actactgccc aatgccgatg ctacaaatgc cggcggcatc gtcaagaaag gaatacccga
     6481 tcagtggctg gacggcatgg ggcgcttcgt caaactgagc ggttatccgt ccaccaatgt
     6541 cagttataga aacggtgtgg cagacgcttc caacaaccag gataaattcg tgacggtttc
     6601 caccaccctc acttgtaatc cgtttttgcc aatcccggtg ccggttttga atgttcccgg
     6661 tctgaacgga cccatgactt tcaccgtcac ttccgagtgt caactggaaa acccggacta
     6721 cgcaccttaa ggggaaagaa ctgtgagaac gcatagacgc aggggacaca gcatgattga
     6781 agccgtcagc gcggccatga tcattattcc tgtcaccctg gttttgctag acctattggt
     6841 gctggtgatt gccaattcca tgaacgatag cgccgcaaaa aacgccgcca gagcagcagc
     6901 caatcagtcg gatgcagcta gcgcctttaa tgccgccaac aaatctctgg agtccttcaa
     6961 gccctcttcc ataattcccg aactgaaaat tcaaaccttc aattatccca atgcccagga
     7021 tattgtcacc tgcaaaaccc gcatgaaagt tgtgttgcct gtgccctttc ccggctacca
     7081 cca
//
LOCUS       DAOQNZ010000017         6858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4238_length_6858_cov_4.388652, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000017 DAOQNZ010000000
VERSION     DAOQNZ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_6858_cov_4.388652"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..762
                     /locus_tag="PKW73_00580"
     CDS             175..762
                     /locus_tag="PKW73_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71802.1"
                     /translation="MTNLQTVSDHDHSPAAMVLTTALNIEELRAALEERTDFEGGSSA
                     TGLNELRRGLCGGQEWYLRAYEEGGKFFLFRQELQVDRLVKDYLCSLACLRVYRQGDE
                     TVMEVSYDVGPEGQAWISGCLLILSLVGVFALFLLLQSGGLSGLFSPPFIIFLLVSFL
                     IYTSTRYFVRTLTENDTETRRAVVELLKSLGAQDV"
     gene            complement(772..1401)
                     /locus_tag="PKW73_00585"
     CDS             complement(772..1401)
                     /locus_tag="PKW73_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71803.1"
                     /translation="MADFGRSNTDTYIAPPSFNNTLSKQLNQAISGGDRNSDVSESMR
                     TRQGCDAGHALKGMSMGEQLDALRQYQRESKNDSQHLPKIDLKMDQDGKGYEVKAKPN
                     PEENSNKGNKLETILKHREQENGSTAEHSCKNMPLQEKPMMEMPMQKMPPNSSFEGGN
                     SDYKLPMQETLGTQSSGKDSSSAGSDSSLPPPMSDSNTNNGHSSKGSNQ"
     gene            complement(1523..2845)
                     /locus_tag="PKW73_00590"
     CDS             complement(1523..2845)
                     /locus_tag="PKW73_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA71804.1"
                     /translation="MSPLDHKETKSKIADLITIKHFFQVPLSYEQPSNKKANETITLF
                     ARELRSLKDEDGKAAEKPYLLFLQGGPGFESPRPESAEGWIKAVLALDYRVLLLDQRG
                     TGLSSPVCHESLAARDTNAQLHYLKNFRADSIVKDCELIRATITGGRPWTVLGQSFGG
                     FCTTTYLSFAPQGLSGALITGGLPPLCQNPDQVYMALYPRVLEKNELYYKRFPEDGPL
                     LKKLVERLESKQDFLPTGEVLSRRRLLQAGLNFGFTGGGMTALHYLLERAFRPDNDSE
                     LSFYFKSYLTNLLHFNTNPIYALLHEAIYCQGQASNWSAERLLSHFPQFNEQADPPLF
                     TGEMIYQWMFEEYNCLKPLRAVAEKLAEYEDWGRLYDPSQLSKNTVPCAAALYFNDMY
                     VDRELSKQTASQIKDIKLWITNEFEHDGLRRNGDTIVPRLLEMAQEGP"
     gene            complement(2865..4517)
                     /locus_tag="PKW73_00595"
     CDS             complement(2865..4517)
                     /locus_tag="PKW73_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71805.1"
                     /translation="MNQPSQENEKRTISRFTLVLLVALCLLGALLRVVLLNIGLTLDD
                     ACTASVMEAQNLAEMVRRIKEYEMSPPLYFLALKGLVTMAGINSVTMALPSFIFGTLT
                     IPLTYLLTLTMVGSKHDKQAEARVCALLAAFFQAVSPLAIIYSHEARTYSLAGLLLSL
                     VLLLYWQYFQGPVTKLVGVMLFLAATALVYTHYTAIFYIGILMVLCLLYKDAEAKPSR
                     AAAPALLPLALSLTTLIPWLPVLLYHKNVGTPWADPTPLSHFAHVMAGNLAAMMPLPV
                     VPSYLIVLVSAAAGLLTLPFAVIKKMRGKAPLISPRLNSAVSFKVMSALLIVLISCAA
                     YGYITPYMFGYVRYLSPLVPLTSALLATLLLSVCKGKFRKIVLICLLPACLAGSFWEV
                     RLLAEKDRSGLRQIAREISAGRYKDAVFYTAPDFVGMILDFYLKHDVNLKAEQMPLVY
                     GFTCPEAGTRPIKHEEDVAWCQVKDLNQLHQKLIDGAIEKGKTRLVLIRDTYKPTSKF
                     MPVKELGDSFMQHLASRYTIETTGLYKGSAASYEVTVFKLKN"
     gene            complement(4514..6196)
                     /locus_tag="PKW73_00600"
     CDS             complement(4514..6196)
                     /locus_tag="PKW73_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71806.1"
                     /translation="MTSISDSESDSTDGEEGSKSNALPEAKAGSNSLLGFYSAGALLL
                     TILGCLQYCLNPLAIVGDTAMYLQCGQFLKEGLLPYVDFQDLNPPLIMYLSLLPAMLA
                     PALGPFAAALSPAVSAQGILPGFQLALSGALFTGAYCLLSLALCYGVLRKLNGAGLDT
                     TLLTPPTLLAPVLVNALAGLDYGQREHLYCLFLLPFVLLRYGRFQCREQGLSQWKAGA
                     LEGCVGFLMGLMLCFKPHFLLPPFLLELYWLARYRKPALLFCRETLTALLPMVLYGLH
                     FLFLPAAVREIYFHYMAPLIVRGYAAFNIVRDTVVNLIWRFALGAYFLALPCLLLLPR
                     RLGLRAPLAILLSACLANIELQQKFWNYHALPLYLFECFIFVAVLAVWLAKLPKVKPR
                     LKALALLLAVLLFQSLNHFFTDSVEAHWLRPWETVLAKTARPGDRALLLDTSDTPWFK
                     TALKFSLAPGSRYLWLFACPMLEYQIRTARSETEKTRAQAQLAELFSNTRADIVRMKP
                     RFILVRRREAFGMGDVDFLEYLKNHGLAGELAACRVLQDSGDFILLEREASR"
     gene            complement(6554..>6858)
                     /locus_tag="PKW73_00605"
     CDS             complement(6554..>6858)
                     /locus_tag="PKW73_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNA71807.1"
                     /translation="PTELMLKGVQLELETRTVKVGPQNVHLMPKQCALLEYLMRHPNR
                     PFGAKALLDAVWPSDSEASEDTVRTCMKTLRRQLATVGKEDFIKTVLGSGYLIEDK"
BASE COUNT         1542 a         1664 c         2006 g         1646 t
ORIGIN      
        1 gaccacagcc cagtctgatt ttcagtttcc cggaaccttc aagttatcga cacgtttgga
       61 caagccggct tatctggtct tgagtttgag gcgtggccct ggcgcggcca atttgcctga
      121 tcagatagaa cttaagtcac tcaagattga aacccactga taaggagacc cggtttgacg
      181 aatttgcaga cagtttctga tcacgaccat tctcctgcag ctatggtttt gaccacagct
      241 ctcaatattg aagagttgag agccgctctt gaagaacgaa ctgattttga aggaggttct
      301 agtgccacag gcttgaacga actcaggcgg ggtctatgtg gtggtcagga atggtacctg
      361 agagcctacg aggaaggtgg taaatttttc cttttccggc aagagttaca agtcgatcgt
      421 ctggtaaagg attacctttg ctccctggcc tgtttgcggg tctatcggca gggcgatgag
      481 accgtgatgg aagtctccta cgatgttggg ccggaaggtc aagcctggat ttccggctgt
      541 cttttgattc tcagcctggt tggtgtgttc gctcttttcc tgttgcttca aagtggcggt
      601 ttgtcggggc tattcagtcc gcctttcatt atttttcttc tagtgtcctt cttaatctac
      661 acatcgaccc gctactttgt tcgtacgctc actgaaaacg acactgagac ccgtcgagcg
      721 gtggtggaat tgcttaagtc gcttggtgcg caggacgttt gattttctgt cttactgatt
      781 tgagcccttg ctgctatggc cgttattggt attagaatcg ctcatagggg gcggcagcga
      841 ggagtctgaa ccggcgctgc tactgtcctt accggaggac tgtgtgccta aggtctcctg
      901 catgggtaat ttgtagtcgc tgttaccacc ttcaaaggat gaattgggtg gcattttctg
      961 cattggcatc tccatcatgg gcttttcttg cagtggcatg tttttgcagg agtgttcggc
     1021 ggtgctgccg ttctcttgct cccggtgctt gagaatagtt tccagcttat tacctttatt
     1081 gctgttctct tccgggtttg gtttggcttt cacttcatag cccttaccgt cctgatccat
     1141 cttcaggtca atttttggta aatgctgaga gtcgtttttt gattctctct ggtactgacg
     1201 cagggcgtcc agttgttcac ccatgctcat gcctttgaga gcgtgaccgg cgtcgcaacc
     1261 ctgtcttgtg cgcattgatt cggagacatc cgaatttctg tcgccgcccg atatagcctg
     1321 gttgagttgc ttcgagaggg tattattaaa actgggcggc gcgatatagg tgtcggtatt
     1381 ggatctgcca aagtcagcca tgatagcctc cagtcaaaga aaacagtaag agtgaagttg
     1441 agcgcgtggt cttttcttga atgcaattag accgccggct gcgtgaaaat cactggaaag
     1501 tttcttgacc ggtgcttccc cctcaggggc cctcttgcgc catttcgagc agccgcggca
     1561 cgatagtgtc gccgtttcgc cgcagtccgt cgtgctcaaa ctcgttggtg atccagagct
     1621 tgatgtcttt aatttgagag gccgtttgct tcgagagttc ccggtctaca tacatatcat
     1681 tgaagtagag ggcagcggcg cagggcacag tgttcttgct gagctgcgag gggtcgtaga
     1741 gtctgcccca atcttcatat tcagccagct tctcggctac tgctcgcaaa ggtttgaggc
     1801 agttgtattc ttcgaacatc cactgataga tcatttcgcc ggtgaaaagc ggcgggtctg
     1861 cttgctcgtt gaactgaggg aagtgactga gcaggcgctc tgccgaccag ttggaagcct
     1921 gcccctggca gtaaatggct tcatggagga gggcataaat ggggtttgtg ttgaagtgca
     1981 aaagattagt caggtaactt ttgaagtaga aggaaagttc actgtcgtta tccgggcgga
     2041 aggctctttc caaaaggtag tgtagagccg tcatgccgcc gcctgtaaag ccgaagttga
     2101 gaccggcttg caacaagcgt cttcggctga gaacttcgcc ggtgggtaga aagtcctgtt
     2161 tgctctcaag ccgttccaca agtttcttga ggagcggtcc gtcttccgga aagcgtttgt
     2221 aatagagttc attcttctcc agcacacgcg ggtagagggc catatagact tgatccgggt
     2281 tttggcaaag tggcggcaga cctcccgtga tgagggcgcc gctcaagccc tgcggcgcaa
     2341 aggaaagata ggtggtggtg cagaagccgc cgaagctctg cccaagaacc gtccatggtc
     2401 tgccgccggt gatggtggcg cgaatcaatt cacagtcttt gactatcgaa tcggcgcgga
     2461 aattcttgag gtaatgcaat tgcgcattgg tgtctctggc agccaggctt tcgtggcaaa
     2521 ccgggctact gagaccggtg cctcgctgat ctagcagtaa gactcggtaa tccagggcca
     2581 gaacggcttt gatccagccc tcggcgctct cgggccgggg tgattcaaag ccggggccgc
     2641 cttgcaaaaa tagtaggtaa ggtttctccg ccgctttgcc gtcctcgtcc ttgaggctgc
     2701 gcagttccct ggcaaagagg gtaatcgtct cattcgcttt tttgttggag ggctgctcat
     2761 aagaaagcgg cacctgaaag aaatgcttga tggtaatcag atcggctatt ttgctcttag
     2821 tttctttgtg gtccaatggc gacaatttcg attcctctta tagatcagtt ttttagtttg
     2881 aagacggtaa cttcatagga ggcggcgctg cctttgtaga gcccggtggt ttcgatggta
     2941 tagcgagaag ccagatgctg catgaaactg tcgcccagtt ctttgacggg catgaattta
     3001 gaggtcggct tgtaggtgtc tctgattaga accaggcgtg tcttgccttt ctcgatggcg
     3061 ccgtcgatta gtttctgatg caactgattg aggtctttga cctggcacca ggctacatct
     3121 tcttcgtgct tgatggggcg ggtgcccgct tccggacagg taaagccgta aaccagaggc
     3181 atttgctccg ctttcaagtt gacgtcatgc ttgaggtaga agtcgaggat catgcccaca
     3241 aaatctggtg ctgtgtagaa gacggcatct ttgtagcgac cggcgcttat ctctctggct
     3301 atttgcctga gaccggaacg gtctttctcg gccaaaaggc gaacctccca aaaggagccg
     3361 gccaggcagg cgggcagaag acagattaag actattttgc gaaatttgcc tttgcagacc
     3421 gacagcagta aagtggctaa aagcgccgat gtgagcggca caagggggct caagtagcgc
     3481 acatagccga acatataggg ggtaatgtag ccgtaggcgg cgcaggaaat taacacgatc
     3541 aaaagggcgg acatgacttt gaagctgacc gctgaattta gccgagggct gatgagcggt
     3601 gctttccctc tcattttttt gatgaccgcg aagggcaggg tgaggaggcc tgcagcggcc
     3661 gagaccagaa cgatcaaata ggatggtact accggtagag gcatcatggc cgccaggttc
     3721 ccggccatga catgggcgaa gtgactgagc ggcgtgggat cggcccaggg cgtgccgaca
     3781 tttttgtgat agaggagcac cggcagccag ggaataagcg tggtcaggct gagggccagt
     3841 ggcagaaggg ccggggcggc cgctcggctg ggctttgctt cggcatcttt gtagagcagg
     3901 cagagcacca taagaatgcc tatatagaag atggccgtat agtgcgtgta aaccagggct
     3961 gtggcagcca ggaagagcat gacgcctaca agcttagtaa ccggaccttg aaagtactgc
     4021 cagtagagaa gcagcaccag gctgaggagc aagccggcca gactgtaggt acgcgcttcg
     4081 tggctgtaga ttatggcaag cggcgaaact gcctggaaaa atgctgccag aagggcgcag
     4141 accctggctt ccgcttgctt gtcgtgcttc gagccgacca tggtcagggt gagcaggtag
     4201 gtaaggggga tggtgagggt accgaaaatg aaagagggta aggccatggt caccgaattg
     4261 atgccggcca tggtcaccag ccccttgagg gctagaaagt aaaggggcgg gctcatctca
     4321 tattctttga tgcggcgtac catttccgcc aagttctggg cttccatcac actggcagtg
     4381 caggcgtcat ctagagtcag accgatgttg aggaggacga cccgcaagag ggctcccaga
     4441 aggcagagcg ctaccagcaa gaccagggtg aaccggctaa tggtgcgctt ttcattttcc
     4501 tggctgggct ggttcaccgg cttgcctcgc gctctaagag aatgaagtcg ccgctgtcct
     4561 gcaagacgcg acaggcggca agctcgcctg ccagaccatg atttttcaag tattccagaa
     4621 agtctacatc gcccatgccg aaagcttccc ggcgtctgac caggataaag cgtggtttca
     4681 tcctgactat gtctgctctg gtattgctga agagttctgc taactgagcc tgtgccctgg
     4741 ttttctcagt ttcgcttctg gctgtgcgaa tttggtattc gagcatgggg caggcgaaaa
     4801 gccagagata tcttgaaccg ggtgccagcg aaaatttcag ggcggtctta aaccagggtg
     4861 tatcggatgt atcaagcagc agggcccggt cgcccgggcg ggctgttttg gccagcacgg
     4921 tctcccatgg tctcaaccag tgtgcttcca ccgaatcggt aaaaaagtgg ttcaggctct
     4981 ggaaaagaag aacagcaagt aagagcgcca gagccttgag ccggggcttt acctttggca
     5041 gtttggccag ccagaccgca aggactgcca caaaaataaa gcactcgaaa aggtagagcg
     5101 gcagggcatg atagttccaa aatttttgtt gtagttcgat attggccaga catgctgaaa
     5161 gcaaaatagc cagtggcgct ctcaaaccga ggcggcgcgg cagcaagagc aggcagggca
     5221 gggccaggaa gtaggcgccc agggcaaagc gccagatcaa attaaccacg gtgtctctca
     5281 ctatattgaa cgccgcatag ccgcgcacta tgagtggtgc catgtaatga aaatagattt
     5341 cccttacggc ggcaggtaag aagagaaaat gtagcccata aagaaccatg ggcagaaggg
     5401 ccgtcagggt ttcacggcag aaaagcaaag ccggcttgcg gtatctggcc agccagtaaa
     5461 gctcaagcag gaagggcggc aggaggaagt ggggtttgaa gcagagcatc agtcccatga
     5521 gaaaaccaac gcagccctcc agggcgcccg ctttccactg gctaagacct tgctcgcggc
     5581 actgaaagcg gccgtagcgc aaaagcacga agggcagaag gaagaggcag tagagatgct
     5641 cccgctgtcc ataatcgagg ccggccagag cgttgaccag caccggcgcc agcagggtag
     5701 gcggcgtcag cagggtggtg tcgagaccgg cgccattgag ctttcgaaga accccgtagc
     5761 aaagcgccag cgaaaggagg cagtaggcac cggtgaacaa tgcaccgctc agggctaatt
     5821 gaaatcctgg caaaattcct tgcgcactta ccgccggact gagcgcggcg gcaaaggggc
     5881 cgagggccgg cgccagcatt gccggcaaga ggctcaaata catgatcagg ggcgggttca
     5941 ggtcttgaaa gtccacataa ggcaaaagcc cttccttgag gaattgacca cactgcaggt
     6001 acattgccgt atcaccgaca atggccaggg gattgaggca gtattgcagg caacccagaa
     6061 tcgtaagcag gagggcgcct gcgctataga agcccaggag gctatttgac ccggctttag
     6121 cctcaggcag tgcgttagac ttggaacctt cctcgccgtc agtagaatcg gattcggagt
     6181 ctgaaattga ggtcaagtct gagtgctttc cttttggaag gtcccggcgt acttctagat
     6241 tctacttgga tttgagggct gaaatttctt ctgacttctt tcttctgcgg ttgaaagttt
     6301 cacaattcgt ttacgtgacc tggattattc tcttaccacg aagcaagcaa ttaccgctca
     6361 cttcaccgtt gccagggtgt taccccagac cactctgagc aagcaaaaag ccgacgagat
     6421 ctactagatt tctggaagtt tccttggggg agcttggctg gcgcctaaaa acgccagcca
     6481 attactatct acataagacc gacggcacga agggctgctt caatgatagc cccggttgtg
     6541 ctcacacttt cggctacttg tcttcaataa ggtagccgga acccagcact gttttgatga
     6601 agtcttcctt gccgacggtg gccagttgtc ttctcaaagt cttcatacag gtgcgcacgg
     6661 tgtcttccga agcttcgctg tcggagggcc agactgcatc cagcagggct ttggcgccga
     6721 aggggcgatt gggatggcgc attaaatatt caagcagggc gcactgtttg ggcattagat
     6781 gcacattctg aggaccaacc ttgacggttc tggtctcaag ctccaattgc acgcccttga
     6841 gcattaattc ggtgggca
//
LOCUS       DAOQNZ010000018         6820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4274_length_6820_cov_4.006652, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000018 DAOQNZ010000000
VERSION     DAOQNZ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4274_length_6820_cov_4.006652"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1076)
                     /locus_tag="PKW73_00610"
     CDS             complement(72..1076)
                     /locus_tag="PKW73_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71808.1"
                     /translation="MNALNKIAIPLVLLPLSLIFSAVLPGEPSLCRAAARDVKGNAAE
                     DAGEVPMPEGEEKAPLKQNKKLIKNQAKEAKALKIKGDSQEKNEKKKEEKAKEEKVKE
                     EKSKVKEGSPEDLAAQALEAEYQALQKQLAALLKIVKTPFTAPSQESENEKPDGLGSF
                     KLAADSSKPALRLSDRLLASRLYLPGRLVIGKPTQFTVKGKPGYWAAVAMADRDSGAK
                     PIYGRNLRLGPDRKVVALGKIPEGGVLSLKYFAPVEGDLIGQSLYFEAALWPDGKPEA
                     VEIAQTVSSEAQSEKANGILVTADTEKKKGVRLVPDSAIPPSQRSLGGGGATLDSGKP
                     "
     gene            1295..1561
                     /locus_tag="PKW73_00615"
     CDS             1295..1561
                     /locus_tag="PKW73_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71809.1"
                     /translation="MLKAAHHNLYIYQKLRDWFLDHRTLFQAFWVALSLHVALLPVLW
                     VAGWALPWPRSPIFTTVVEYDLRGWPNLAKPKKIYETIHFDGAK"
     gene            1969..4650
                     /gene="recJ"
                     /locus_tag="PKW73_00620"
     CDS             1969..4650
                     /gene="recJ"
                     /locus_tag="PKW73_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNA71810.1"
                     /translation="MKQTGDTAREKSGDKALAKVWIYPTSNAPDSTLVEACGGSVVLA
                     KVLLRRGFETVEQIRDFLDETIYKPSHFSELPNTDKAVTRLAAAIEKKEKITVYGDYD
                     VDGITGTSVLLSVLRKLGANVDFYIPNRLSEGYGLNLKAVSVLASKHRTKLIVTCDCG
                     VSNFAEINLAKSLGVDSLVLDHHTMPEMLPPAVAVVHPKLLPESHPLFHLPGVGVAYK
                     VCEGLLSHFDRAEEADELLDFVTLGMIADLVPLVKENRYLVKIGLPRLVKSPRIGLKA
                     LLQQVGGKEDTDLVGFGLAPRINAVGRLADAASAVELMTTEDEGRADELAKQLARENL
                     RRQELCEKVFMEAERQIQNTCDLNNDKGICIYNEEWHHGVVGIVASRLVEKFQRPVFI
                     GQLDKEEGLVRGSARGVEQIDLYEVLKDNEHLLLKWGGHKMAAGFSIEADKAEAAARG
                     IVNSCNKFVGNKSLRSRVEIDALVEGDSLALSTVRELNAMAPFGMNNKKPILCMEKLT
                     VASVKPLGKEAKHSRIMVHDEAAPGSADFECVMWNSRGRVPVDGELVDLAFNADINAY
                     GGRERLQLVLVDWRKHGSQMEAAASESEAVKNRAGISPAPVRAVVEVDQPEAPGTAVS
                     DGDEALDEDSEGDVAALAEAGNLVKSQALVRVNFKDLRAYAGNPEVIKKAIGKLSGGV
                     FVYGESCPKLKGIEFQDRTQVSAVDNLLIWQFPPSLKNFQDLLFATRAKNIYLVGSVS
                     DDDLMPEDPANFLKQLLGLVRFAVNQRDGQVDGEKLAAVLGVTKMSIALALAALKRVH
                     WIDWFSENGTIYLDLLGSPVASVEDTPEYKQLVASLDQTDKFRKWCCQCTMKELQLAL
                     MPNQIGFAAEPLVRGEAGSAEGPQIKSVTISGPGEEI"
     gene            4692..5276
                     /locus_tag="PKW73_00625"
     CDS             4692..5276
                     /locus_tag="PKW73_00625"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNA71811.1"
                     /translation="MTAKVEQPRLMEKLPLSPERQDWLKSTIRDIPDFPKPGIIFKDL
                     TTLMRNAEAFSCVLNSLSVTSEALKPTVIVGIEARGFILAPAVAHHLGVGFVPVRKPG
                     KLPWHTERVSYALEYGEDTVEVHRDAVTKGDRVVIIDDLLATGGTAMAAKQLMEKLGA
                     TVVGAGFVVELDFLDGRSRVKKDGDLEIFSVIQY"
     gene            5334..>6820
                     /locus_tag="PKW73_00630"
     CDS             5334..>6820
                     /locus_tag="PKW73_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71812.1"
                     /translation="MVANKGNLNKSQTALMAALAFACLVFQSGCRKEALPSAAERQTK
                     HAQFLARIDSEDAEQAALYRHSQQVAGDPLPLLKLPDEKQSDFQKAREIVGSIKNCPS
                     LTVGLFPDYREIWHTYDQSALNYSRRDYETIRSLARSSAGLSLMFATQCHHLEEHFRS
                     WTGNPILAGLQARARKDFGKCDGFVVNELNYADYLALLPRSMAHEGEDVSGAFVKKID
                     VAAWRKKYGAKPVDAFLNALSLAQKEKGRTREVLYFTLPKNNWADRLSAVKPQLQALG
                     YEVYGAGGFEQLHCFTTAQECQKFVTAGAMREGSYKQWHAVASKLVLDPEGKILALEG
                     DVGRASILKYGEGSLSASQKILLAADSYIQSLVRIGDDEAVAERFVFRGFESDCLPPG
                     ARIAKVKEGFEITVPERCYLQVPVSRAFLVKDLNQYAYLEAQGLETVAHGKCFESVVA
                     RLRKWDRLYGLKVLEANYNSLVVKFDRLPADITSLCVDIRDLCPGA"
BASE COUNT         1469 a         1728 c         1910 g         1713 t
ORIGIN      
        1 agggtaggtg taggtgaccg gcctgtcgtc gaggggcact agcaaaattc gcatgggact
       61 tccttattga actatggttt gcctgagtcg agagtggcgc ccccgcctcc caggcttctc
      121 tgggagggag gaatggctga gtcgggtacc agtcgcaccc cttttttctt ttcggtgtcg
      181 gcggtgacaa gtatgccgtt ggctttttcg ctctgggctt ccgatgaaac ggtctgagcg
      241 atttccaccg cttccggttt gccgtccggc cagagggctg cttcaaagta gaggctctgg
      301 ccgatcaagt cgccttccac cggagcgaaa tatttgaggg agaggacgcc gccttctggt
      361 atcttgccaa gagctaccac tttgcggtcc ggtccaaggc gcaggtttcg cccgtaaatt
      421 ggttttgcac cgctgtcccg gtcggccatg gctactgccg cccagtagcc gggtttgcct
      481 ttgacggtaa attgggttgg tttgccgatc accagtcggc ctggcagata aaggcgggag
      541 gcaagcaggc gatcggagag cctgagagcg ggcttggagc tgtctgcggc cagtttgaaa
      601 gagcccagtc cgtcaggttt ttcgttctcg ctttcctggc tgggggcggt gaaaggggtc
      661 ttaacgattt tgagcagggc ggccagttgc ttttgcaggg cttgatactc agcctccagg
      721 gcctgggcag ccagatcttc cggactgcct tctttgacct tgctcttttc ttcttttact
      781 ttctcttcct tggctttctc ttctttcttc ttttcgttct tttcctggct gtcgcctttg
      841 attttgagag ctttggcttc ctttgcttga ttcttgatca atttcttgtt ctgtttcagg
      901 ggagctttct cctccccttc cggcatgggc acttcgcctg cgtcctcagc ggcattcccc
      961 ttgacgtctc gggcggccgc ccggcaaagg ctaggctctc ccggtagaac ggcgctgaag
     1021 atcaggctca gaggcaggag gaccagcggg atggcaattt tattgagcgc attcattggt
     1081 tcggcttgat taaatttaga gcttggcgcg ggatctcagg ggggctcaga gctaccctgg
     1141 tgagtttttc atagtatcac caagtggtcc cttttcgctg ccctttgagg gcgcggcttg
     1201 cctgccctgg ctggtttaac cgtactctcg gggctagccg cggcgcattc ccttgaaaat
     1261 ttcgctaaaa tagggattac cgtaggattt ggccgtgctc aaagctgctc accataattt
     1321 gtacatctat cagaagctta gagattggtt cctagaccat cggacccttt ttcaggcttt
     1381 ttgggtagcc ctcagcttgc atgtggcgct tttgccggtg ctctgggtgg ctggatgggc
     1441 cttaccctgg ccgcgctcac ccatttttac caccgttgtg gagtatgact tgagaggctg
     1501 gccgaatttg gccaagccca agaaaatcta tgagaccatc catttcgacg gcgctaaatg
     1561 aaatcttgct ttgcttaggt ttttgcagac ggctatctta gaaaatgcgt atatgcgttg
     1621 aactatttgt cgcgattgag cgtatagata attgtgggtg gttgtatgtc acttgggagg
     1681 tgagaccggc cggatcgccc gtttcgagag gtcggaagct gagcaactat ttctcttagc
     1741 cgactttacc ttctcattta tcatgctacg attgctttag ctaaaagcgc tttgcatcat
     1801 acttcggtgc atatcgagta ggcttcaaat ctatgttata tgcaacagac atgaagtctt
     1861 gaggcgtata tcttttgttc cttttaattt ggtcccatta tcaacacagt tcaatactgt
     1921 ccgagaacca gtcaaccaga acctatctaa aagtctaagg ctagagctat gaaacaaacc
     1981 ggggacactg ccagagagaa gagcggcgac aaggcgcttg ctaaggtttg gatttatccc
     2041 acaagcaatg cgccggacag cactctggtg gaagcttgcg gcggctctgt ggtgctggcg
     2101 aaagtgcttc tgcggcgcgg ttttgaaacg gtagagcaga tccgtgattt cctcgacgaa
     2161 accatttaca agcccagtca cttttcggaa ttgcccaata ccgataaggc tgttacccgc
     2221 ctggccgctg ctattgaaaa gaaagaaaag atcaccgtat atggtgacta tgatgttgac
     2281 ggcatcaccg gcaccagcgt cttgctgtcg gttttgcgta agcttggcgc caatgtcgat
     2341 ttttatattc ctaatcgcct cagcgaaggc tatggtctca atctcaaagc cgtgagcgtg
     2401 cttgccagca agcaccgcac caagctcatc gttacttgcg attgcggtgt ctcaaacttc
     2461 gccgagatca atctggctaa gtccctcggg gttgattcgc tggtgctcga tcatcacact
     2521 atgccggaga tgctaccgcc ggcggtggcg gtggtgcatc ccaagctgct accggaaagt
     2581 cacccgcttt tccacttgcc tggtgtgggt gtggcgtaca aagtctgcga ggggctgctc
     2641 agtcattttg accgagcgga agaagccgat gaactgctcg actttgttac tctcggcatg
     2701 attgccgatc tggtgccgct cgtaaaggaa aatcgctatc tggtcaaaat tggcttgccc
     2761 aggctggtta agtcgccccg catcggtttg aaggcgctct tgcagcaggt gggcggcaag
     2821 gaagatacag acctggttgg ttttggtctg gctccccgca tcaacgccgt ggggcgtctc
     2881 gccgatgccg cctctgccgt agaactgatg accaccgaag acgaaggtcg cgccgacgag
     2941 ctggctaagc aattggctcg cgaaaacctg cgtcggcaag aactctgtga aaaggtcttc
     3001 atggaagcgg aacgccagat ccagaatact tgcgatctga ataacgataa aggcatctgc
     3061 atctacaacg aagagtggca ccacggtgtg gtcggcattg ttgcttcccg tctggttgag
     3121 aagtttcagc gcccggtctt catcgggcaa ctcgataaag aagaaggcct ggtacgggga
     3181 tctgcccgtg gcgtagagca aatcgatctt tacgaggtct tgaaagacaa cgagcatctt
     3241 ttgctcaaat ggggtggtca caaaatggcg gccggtttca gcattgaagc cgacaaagcc
     3301 gaggccgcag cccggggcat cgtcaacagt tgcaacaagt tcgtgggcaa taagagcctg
     3361 cgttcccgag ttgagatcga tgctctggtg gaaggtgata gtctggctct gagcacagtg
     3421 cgcgaactaa atgccatggc tcccttcggc atgaacaata aaaagcccat cctgtgcatg
     3481 gagaagctga cggtggcgtc cgtcaaaccg ctgggcaaag aagccaaaca cagccgcatt
     3541 atggtgcacg acgaagccgc accgggaagc gccgattttg aatgcgtcat gtggaattcg
     3601 cgcggtcgtg ttcctgtaga cggtgaactg gttgatctgg ccttcaatgc cgatatcaat
     3661 gcctatggcg gtagagagcg tctgcaactt gttctggtcg actggcgcaa acacggcagc
     3721 cagatggagg cggccgcaag tgagagtgaa gcggttaaga accgggccgg tatttctcct
     3781 gctccggtgc gtgccgtggt agaagttgat cagcccgagg cgcccggcac cgctgtttca
     3841 gacggtgacg aagctctcga tgaggacagc gaaggcgatg tggctgctct ggccgaagcc
     3901 ggcaatctgg ttaaaagcca ggcgctggtg cgggtcaact tcaaagactt gcgtgcttat
     3961 gctggcaacc ctgaggttat caagaaggct atcggtaagt tgagcggtgg cgttttcgtt
     4021 tacggcgagt cctgtcctaa gctcaaaggc atagaattcc aagatcgcac ccaggtgagc
     4081 gccgtcgata atttactgat ctggcaattc ccgccttcct tgaaaaactt tcaagatttg
     4141 ctctttgcca cccgggctaa aaatatctac ctggtgggct ccgtcagtga cgacgatctg
     4201 atgccggaag acccggccaa cttcctcaag caactgcttg gtctggtgcg ttttgccgtt
     4261 aatcaaagag acggccaggt tgacggtgaa aagctggccg ccgtacttgg ggtcactaag
     4321 atgtcaatag cgcttgccct tgctgccttg aaacgagtgc actggattga ctggttctcc
     4381 gaaaacggca caatctatct cgatttgctt ggttcccccg tggccagtgt ggaagacact
     4441 cctgagtaca aacagcttgt ggcttccctc gatcaaaccg ataagttccg taagtggtgc
     4501 tgtcaatgca ccatgaaaga gctacaatta gcactcatgc ccaatcaaat tgggtttgcc
     4561 gccgaacctc tcgtaagggg cgaagccggt agtgccgagg gtcctcagat aaagtcggtg
     4621 accatttcag gtcctggtga ggaaatctga gcaccggcaa agccaataca gtaaatagga
     4681 tagtaatcaa gatgaccgca aaagttgaac aacccaggct catggaaaag ttgcccctca
     4741 gtcctgagcg gcaggattgg ctgaagtcca ccatccgtga tatccccgat ttccccaagc
     4801 ccggcatcat atttaaagat ctgacaactt tgatgaggaa tgccgaagca ttctcttgcg
     4861 ttctcaacag cctctctgtc acttccgaag ccctcaagcc caccgtcatt gtcggtatag
     4921 aagcgcgcgg ttttatcctc gctcctgctg tggcccacca cctgggcgtc ggtttcgtac
     4981 cggtgcgtaa gcccgggaaa ttgccctggc ataccgagcg cgtttcttac gccctcgaat
     5041 atggcgaaga cactgtggaa gtgcatcgtg atgccgtcac caagggtgat cgcgtggtca
     5101 tcatcgacga ccttctcgcc accggcggca ccgccatggc tgccaaacag ctcatggaaa
     5161 aactaggcgc caccgtggtc ggcgccggct tcgtggtcga acttgatttc ctggacggac
     5221 gttcccgcgt taagaaagac ggcgacctgg aaatcttctc cgtgattcaa tattaggtat
     5281 tctgagtttg caactttagc tgccctcaga ttcgaacttt gaagggctgt agcttggtcg
     5341 caaacaaggg gaacttgaac aagagccaga cggcactgat ggccgctctg gcttttgctt
     5401 gcctggtctt tcagtctggt tgccgcaaag aggctctccc ttcggcagcg gagcggcaga
     5461 ccaagcatgc ccagtttctc gcccgtatag atagtgaaga tgcagagcaa gcggcgcttt
     5521 accgccattc tcagcaggtt gccggcgacc cattgccgct tttgaaactg cccgatgaaa
     5581 agcagtcgga ttttcaaaaa gccagggaga ttgttggttc tatcaagaac tgtccgtctt
     5641 tgacggtggg tctgtttccc gactaccggg agatctggca tacctatgac cagagcgctc
     5701 tcaactatag ccgtcgggat tacgagacca tcaggtcttt agccagaagt tcagccgggc
     5761 tttcgctaat gtttgctact cagtgccatc acctggagga gcattttcgc tcctggacgg
     5821 gaaaccccat tctggccgga ctgcaggctc gtgcccggaa agacttcgga aaatgcgatg
     5881 gttttgtagt gaatgagctg aattatgcag attatctagc cctcctgccc aggtctatgg
     5941 cccatgaggg tgaggatgtc tctggtgcct ttgtaaagaa aatagatgtg gccgcctggc
     6001 ggaagaaata tggagcgaag ccggtcgatg ccttcctgaa tgccctgtcg cttgcccaga
     6061 aagaaaaggg taggacgaga gaagttttgt atttcaccct tcccaaaaac aattgggcag
     6121 accggcttag tgctgtcaaa ccgcaactgc aggcactcgg atacgaagta tacggcgcag
     6181 gcggctttga gcagttgcat tgttttacca cagcgcaaga gtgtcagaaa tttgtaactg
     6241 ccggcgcaat gagagagggt tcttacaagc agtggcatgc cgtagcttcc aagcttgtac
     6301 ttgatccgga aggtaaaatt cttgcgcttg aaggtgacgt gggcagagcc tctatcttga
     6361 aatatggtga aggtagcttg tctgcgtccc agaagattct cttagctgcc gattcctata
     6421 ttcagtctct tgtccgtatt ggggacgatg aagccgttgc agagcgcttt gtgtttagag
     6481 gctttgagtc tgactgcttg ccgccgggcg ccaggattgc aaaggtcaag gaaggttttg
     6541 aaataacggt gccagaaaga tgctatctgc aggtgcctgt cagtcgtgcc ttcctggtca
     6601 aagacctaaa tcaatatgcc tatctggaag ctcagggtct tgagaccgtt gcccatggaa
     6661 agtgtttcga atctgtggtg gctcgcttgc gcaaatggga caggctctac ggtctcaagg
     6721 tgctggaggc aaactacaat agtttggtcg tcaaatttga tcgtctgcct gctgatatta
     6781 cctcgctttg cgtggacatc agagacttgt gtcccggtgc
//
LOCUS       DAOQNZ010000019         6769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4321_length_6769_cov_3.596366, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000019 DAOQNZ010000000
VERSION     DAOQNZ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4321_length_6769_cov_3.596366"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKW73_00635"
     CDS             complement(<1..885)
                     /locus_tag="PKW73_00635"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNA71813.1"
                     /translation="MKWASVLETPADLSDSGDEGQGTVPELALRLGQSLVKTLLFKLG
                     GVKPDFLVLFVSPDFKDKMELLSTYLQETLAPKAFIGCTGGGLIGGGQEVEHKSAIAL
                     TGATMPGVQLKAFHIANEAIPDLDDAPDAWEKLLQVKGLEEPSFVILADPFSFRIDAF
                     VQGLDYAFSRSVKVGGLASGGHKAGENRLLAGDRVFRSGLVGVSMVGNVTVDSVVAQG
                     CRAFGKVSRVTRCDRNLLYELDGKPAVAVLKEAVEKLSEKDQKLAKDQLFIGVAMDEF
                     KDKLVSGDFLVRSIIGIEP"
     gene            complement(1003..2610)
                     /locus_tag="PKW73_00640"
     CDS             complement(1003..2610)
                     /locus_tag="PKW73_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA71814.1"
                     /translation="MPTENETGVGAPGPGELPFRSPLFGLLQESLAGGHKDREFLYFE
                     GKSYSFAQVHELALRLASLLAREGVARGERVAVYLYNSPEFVVSLLALNYLGAVVVPV
                     NPLLKKDEISHIVHDASAFALLTESHLLAHVAPETIDSLHFHLVFNRDTSVETAVENA
                     FNSPAGSIRINVAALEELDGALPRPSLALSQADDAALIVYTSGTTGKPKGAVLSFGNV
                     AYTVSTYPTRFHLLETDRLLGILPLCHLYGLLVVLAGALRAGASITLMRQFDADKAAL
                     LLKERRITVLSAVPTMHQFILLSLDKLGISLPDLRLITTGGAAISLELLKQVEETFSV
                     PVLEGYALTETSVIATLNPVDNRKAGSVGPGFDLLTIMIQDENGAPLSPGKTNVGEVC
                     IAGPCVMSGYWQNPEATAAAIVNGFLRTGDLGYLDEDGYLYIVGRSKELIVRGGMNIY
                     PREVENVISQLPQVAEVAVFGIPDRFMGERVKACIVLKPNLSLTAEQVQSYCGEHLAD
                     YKMPRTIDFVASLPRNSTGKVLKRLLS"
     gene            complement(2772..3443)
                     /locus_tag="PKW73_00645"
     CDS             complement(2772..3443)
                     /locus_tag="PKW73_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNA71815.1"
                     /translation="MLENFARTESGTADRTEPIKADPGVVMYIELLRQENGLTSVYRC
                     NNLVQFRSGDQIRFHIIPNQDRYAYVLMRGTSGASCLLFPQALTGMDNLVKAGTDCTL
                     PTEGALQFDENPGTEQLVMIFSGKPLKTSFLQKIVERGGGKRLPLRTVVAGARSGGGQ
                     LKFRHLRLLMDTKPELMPLSDISQLQVPLSGDKRKALAGPGLLLVSSRKKATISAAVE
                     LQHLK"
     gene            complement(3527..4261)
                     /locus_tag="PKW73_00650"
     CDS             complement(3527..4261)
                     /locus_tag="PKW73_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNA71816.1"
                     /translation="MKTGKLNLLVAMVSLGLAGTGIVPGQAAPASKVRTLAMSQAGAG
                     TAKAPAKSIEQLHQEVQANPTAAAARLALGQAYMSAKDYVKAREQLRLAVRLGKGSPD
                     AQRANQALMALPHALVKPKTGAETRMIASMLGLGRTRGAGGAARPTVIDFYASWCQPC
                     KQLDGVMGRLLKDSYSDKVKYMRVDVDDPNSQALLDQYEVSPIPTVVFLNSEGEVVSY
                     SVGFSGEHAVRDGLSKITATSGSGTN"
     gene            complement(4265..5356)
                     /locus_tag="PKW73_00655"
     CDS             complement(4265..5356)
                     /locus_tag="PKW73_00655"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA71817.1"
                     /translation="MRKPRPSVFSAVVTSTGASTCRGIADRYKFSNLKDGLAVVVVFC
                     LFLLLSTFLPALAESAGPKAEKQVAAAVKPRENAHNQTRARRGNAPCLSWNDPAVPIK
                     AVFLCVHGLGLHNGTYEPLGKQLSSLGYPVYAIDVRGFGSWMEAKGRECVDFEGCLAD
                     IKSTLKVIRRAHPGKPVFLLGESMGGAIALRAVARYPELVDGLVSSVPAGDRFKQGQT
                     SLKVALHFLAGPDRPMNVGESVIKQATDKPELRAAWQNDPLARMKLSPKELIQFQSFM
                     NQNHESAREIKDKPVLIVQGVLDKLVRPEGTVELFNKLSTTDKEIALIPNGEHLIFEE
                     NQFSDQVVEKLVAWVELHRKNDASAIIKE"
     gene            5704..>6769
                     /locus_tag="PKW73_00660"
     CDS             5704..>6769
                     /locus_tag="PKW73_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71818.1"
                     /translation="MSKSLSQKLDIYDSSRVGKEALALPRLPVRQALLGSVFLTALAD
                     WLFILSLGSTIVFTGPGGDTSGCLLLMVPLYIFSIFMVITQMLKERMNEIKGTVALPE
                     NSSAKRRLDKAKLLRLVFALAMTQPQMRQFFLSSPQNTICILCLYMLSAQWSLLFVLH
                     HVRPWQGLSTAALSYLIAAWGAGQALAFKLAPAMVNSYPGLWPSQAAALLYSLGICLV
                     YFADRENQFAEATEGSITAEGTQNKPDYYPHGLMVGLMSMPTLCLLIITAFFSVQNLS
                     MQMSGQQCQPLTELAAKLITAFSLGALIAPIIENRVEQKKLWLFSSLLTLSLYLAGAL
                     ARESALSSFSLVTAAALTGSL"
BASE COUNT         1667 a         1955 c         1667 g         1480 t
ORIGIN      
        1 gggttctata ccaataatgg agcgcaccag gaagtcgccg gagacaagtt tgtccttaaa
       61 ctcatccatg gccacaccga taaagagctg atccttggcc aatttctgat ctttttccga
      121 aagtttctcc acggcctcct tcagtacggc cacggccggt ttgccgtcca gttcgtataa
      181 gagattgcga tcacagcgag ttacccgact gaccttgccg aaagccctgc agccctgggc
      241 taccaccgag tcaaccgtga cattgccgac catggagacc cccacaagac ctgaccgaaa
      301 aacacggtcg ccggccagta agcgattttc ccccgccttg tgtccacccg aggccagtcc
      361 gcccacctta accgaacggc tgaaggcata atcgagcccc tgcacaaagg catcgatgcg
      421 gaagctgaaa ggatcagcca gaattacaaa actgggctcc tccagcccct ttacctggag
      481 caatttctcc caggcgtccg gcgcgtcgtc aaggtccggt atagcctcgt tggcgatatg
      541 aaaggctttc agctgcaccc ccggcatggt cgcaccggta agcgctatgg ccgacttatg
      601 ctcgacttcc tgcccgccgc cgattaaacc gccgccggta caccctatga aagccttggg
      661 tgcgagggtc tcctgcaggt aagtggaaag cagttccatc ttgtccttga aatcaggcga
      721 aacaaagagc acaagaaagt caggcttcac gccgcccagc ttgaaaagta gggtcttaac
      781 cagtgactgc ccgaggcgca aggccaactc aggcacagtg ccctgcccct catcgccgct
      841 gtccgaaaga tccgctggtg tttccaaaac ggaagcccac ttcacaatta aatctcccgt
      901 ccattccaag aagacaacca attagatctt aactgaattt acccgccctg accaagcaaa
      961 ctataaattg gcggaagctt ggcaacaagc ctctgtcagt gttcaactca aaagtcgctt
     1021 taagaccttg ccggtggagt tgcggggcag agaagcaaca aagtcgatgg tgcgaggcat
     1081 cttgtaatcg gcgagatgct ctccgcaata gctctgcact tgctcggcag taagactgag
     1141 attgggtttg agtacgatac aagccttcac tctttcaccc ataaaccggt caggtattcc
     1201 aaaaacagca acctcagcca cttgcggcag ttgactgata acattctcca cttcccgcgg
     1261 gtagatattc atgccgcccc ggacgatcag ctctttcgat ctgccaacga tatataaata
     1321 accgtcttca tccagatagc ccagatctcc ggttcgcaag aagccattta caatggccgc
     1381 cgccgttgcc tccgggtttt gccaataacc gctcataaca cagggaccgg cgatacacac
     1441 ctctcccaca ttagtctttc caggggagag gggcgctccg ttttcatctt gaatcatgat
     1501 cgtcaaaagg tcaaaaccgg ggccgacgct accagctttg cggttatcaa ccggattgag
     1561 ggtggcaatc acactggttt cagtcagggc gtaaccctca agcacaggta ctgaaaaggt
     1621 ctcttctact tgcttgagca actccagcga aattgccgca ccaccagtgg tgatcagccg
     1681 caaatcagga agagaaatac ctagtttgtc cagagacagg agtatgaatt gatgcatggt
     1741 cggcaccgcc gacaataccg taatgcggcg ttccttcaaa agtagagcag ctttatcggc
     1801 atcgaactgc ctcatcaaag taatggaggc tcccgctctc agtgcgccgg ccagcaccac
     1861 cagtagacca tagagatgac agagcggcaa aattccaagc agccgatctg tctcaagcag
     1921 atgaaagcgc gtgggatagg tagagaccgt ataagccaca ttaccgaagc tcaataccgc
     1981 ccccttaggc ttgcctgtgg taccactggt ataaacgata agggcggcat catcagcctg
     2041 ggaaagagca agcgaaggtc tcggcaaggc accgtccaat tcttccagcg ccgccacatt
     2101 tattctgatt gaaccggcag gcgaattaaa agcgttctcc accgcggtct cgaccgaggt
     2161 atccctattg aatacgagat gaaagtgcag actgtcgatg gtttccggcg ccacatgggc
     2221 aagtaaatga ctttcggtca aaagggcgaa cgccgaggca tcatgaacga tatggctgat
     2281 ttcgtctttc ttgagaagag gattgaccgg cactaccacc gcgcccagat aattcagggc
     2341 caaaagtgaa acaacaaact caggactgtt atatagataa acagccaccc tctcgccccg
     2401 ggccacaccc tctcgagcaa gaagcgaggc cagcctcaga gctagttcgt gcacctgggc
     2461 gaagctataa ctcttgccct cgaaatagag aaattctcta tctttatgtc cgcccgccag
     2521 tgactcctgc aaaagaccga aaagcggact tctaaagggc aattcaccag gtcccggcgc
     2581 accgactccg gtttcattct cggtaggcat aaatactgac ataacctgca aaaccgcatt
     2641 cagagggggg atcgaggttt cctccaggtt tgccccggcc ccaggggatt ggcgccactg
     2701 cttggtgcaa acaatcctgc tcttcagacc aggcaaactg taagcccttt catgagggct
     2761 agagaatcat actatttaag atgctggagt tccactgcgg ccgaaatcgt tgccttcttc
     2821 ctggaagaga ccagaagaag acccggcccg gccagagcct tacgcttatc tccgctcaag
     2881 ggcacctgca gttgggagat atcggatagg ggcattagct caggcttggt atccatgaga
     2941 agccgcaagt gccgaaactt caattgcccg cccccgctcc gggcacctgc taccacggtg
     3001 cgcaacggca gacgttttcc tccaccccgc tccactatct tttgcaaaaa ggaagttttg
     3061 agcggcttgc cactaaaaat catcacgagt tgctctgtgc cggggttctc atcaaactgc
     3121 aaagctccct cagtgggcaa ggtgcaatca gtgccggcct tgaccagatt atccataccg
     3181 gtcaaagctt gcgggaaaag caggcaactg gcgcctgaag ttccacgcat cagcacataa
     3241 gcatatcgat cttgattggg aatgatgtga aagcgtattt gatcgccgct tctgaactgc
     3301 accaggttat tgcagcgata aacggaggtg aggccgttct cctgacgcaa aagttcgatg
     3361 tacataacca cgcccggatc tgcttttatc ggctccgtcc ggtctgccgt tccagactcg
     3421 gtgcgggcga aattctcgag catcggctct gacggctcca gggaaaagca cccctcaccg
     3481 cctgaccagc acagacaagc aaagagtatg gcaaggattc ggcgcattaa tttgtgccac
     3541 ttccactggt ggcggttatt ttgctcagac catcacggac ggcatgctca ccggagaagc
     3601 ccaccgaata ggaaaccact tcgccttcac tgttgagaaa gactacggtg ggaatggggc
     3661 tgacttcgta ctgatcaagc aaagcctggc tgtttggatc atccacatcc acgcgcatgt
     3721 actttacctt gtccgagtaa ctgtccttga gcaagcgacc catgacacca tcgagttgct
     3781 tgcagggctg acaccaactg gcatagaagt cgataactgt aggtctggca gcaccaccgg
     3841 cgcccctggt acggccaagt cccaacatgg aggcaatcat tctggtttcc gctccggtct
     3901 taggcttgac cagagcgtga ggcaaggcca tcagggcttg attggcgcgc tgagcatccg
     3961 gcgagccttt gccgaggcgc acggccagac gcaactgctc cctggctttc acataatctt
     4021 tggcggacat ataagcctgg ccaagagcca gtctggcagc agcagcagta gggttagcct
     4081 gaacctcctg atgcaattgt tcaatgctct tggcgggagc tttggctgta ccggctcccg
     4141 cctggctcat ggccagtgtt ctcaccttac tggccggagc ggcctgaccc ggcacaatac
     4201 cagtgcccgc cagacccaga ctaaccatag ccaccagtaa gttcaacttt ccagtcttca
     4261 atttctactc cttaataatt gcactcgcat catttttgcg atgtagttca acccaggcaa
     4321 cgagtttctc taccacctga tccgagaact ggttttcttc aaaaattagg tgctccccat
     4381 tggggattag agcaatttcc ttatcggtgg tggaaagctt gttgaaaagt tccaccgtac
     4441 cttcgggtct caccagcttg tcaagcactc cttgcacaat caaaacaggc ttgtccttga
     4501 tttccctggc gctttcgtga ttctgattca taaatgattg gaactgaatc agctccttgg
     4561 gagaaagctt cattctagcc agcggatcat tctgccaggc cgctctcagc tcaggcttgt
     4621 cagtagcttg tttgatgacg ctctcaccta cattcatggg gcgatcaggc ccggccagga
     4681 agtgcaaggc cactttcaaa cttgtctgtc cttgcttgaa gcgatcacca gccggcacag
     4741 acgaaacaag cccatccacc aactccggat aacgagccac cgccctgagt gcaatggcac
     4801 cacccatgga ctcaccgagt aaaaatacag gcttaccggg atgggctcga cgaataactt
     4861 taagcgtact ttttatgtcg gccagacagc cttcgaagtc cacgcactct cttcctttgg
     4921 cctccatcca ggagccgaag ccccgcacat ctatggcata gaccggataa ccaagggagg
     4981 aaagctgctt accgagcggc tcataggtgc cgttatgcaa acccaggcca tgtacacaaa
     5041 ggaagacggc cttgatcggt acggccggat cattccaact caaacagggg gcattaccac
     5101 ggcgcgcccg ggtctgatta tgtgcatttt ctctgggctt aaccgcagcc gccacttgct
     5161 tttcagcttt cgggccggca ctctcagcca gcgccggcaa gaaggtagaa agaagcaaaa
     5221 acagacagaa aactaccaca acagccaaac catccttgag gttggagaat ttataccggt
     5281 cggcgatacc tctgcaggta ctcgcaccgg tactcgtaac aacggcacta aaaacggagg
     5341 ggcgcggctt tctcatgacc ggaattgtct cactctttgg ccgagtctgt aagcgcatga
     5401 ttttaaccaa gagcacaggc accaatctca gatggtgcct gccgcaggcc cggaaaggcg
     5461 acatatacac tggataagga gtcacactct caagctgtgg gacatggtca gcaagggcac
     5521 ttgtcgtgcc cttgcaaaaa tttgaatgaa ttaatatggg tgaggcggca gtctgcagta
     5581 ggggctccag atcaaaacga cagtgtggat cgaccctcgg cacttcacga ctaaaaaaac
     5641 aaagccacaa aaactcttca aaaataattt agagaagctg ccctttgcct tcaaaatagg
     5701 caaatgtcca agagtctttc tcaaaagctt gatatatacg acagtagcag agtcggcaag
     5761 gaggctttag ctctgcctcg tctaccggta cgccaggctc tcctgggttc ggtatttttg
     5821 acggccctgg cggattggct ctttattctt tccctcggct ccaccatcgt attcacggga
     5881 cccggcggcg ataccagtgg ctgcctactc ctaatggtac cgctgtatat attctcgatt
     5941 ttcatggtca tcacgcagat gctgaaagag aggatgaatg agataaaagg cacggtagct
     6001 ctgccggaga attccagcgc caaaaggcgt ctggacaagg ccaagctctt gcgactggtc
     6061 ttcgccctgg ctatgacgca gccgcagatg cgacaatttt tcctttcctc accccagaac
     6121 acaatctgca ttctctgcct ttatatgctc tcggctcagt ggtcgctttt gttcgtgctg
     6181 catcacgtcc ggccctggca agggctgagc accgctgccc tgtcctacct gattgccgcc
     6241 tggggagcag gccaggccct ggccttcaaa ctggcaccag ccatggtgaa tagttatccc
     6301 ggactctggc cctcgcaagc ggctgccttg ctctacagcc tgggaatttg tctggtctac
     6361 ttcgccgacc gggaaaatca atttgccgaa gccactgaag gcagtatcac cgccgagggt
     6421 acgcagaaca agcccgatta ctatccgcac ggactgatgg tcgggctcat gtccatgccc
     6481 acactttgcc tgctcataat cacagccttc ttctcggtac aaaacctcag catgcaaatg
     6541 agcggtcaac aatgccagcc cctcacagaa ctggctgcta aattaattac ggccttcagc
     6601 ctgggagccc tgatcgcacc aatcattgaa aatcgagtcg aacagaagaa actctggctt
     6661 ttctcatctc tgctcacact ttcactttac ctggccggcg ccctggcccg ggaaagcgct
     6721 ctttccagct tcagcctggt gacggcagcg gccctgacag gcagcctgg
//
LOCUS       DAOQNZ010000020         6698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4377_length_6698_cov_3.874906, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000020 DAOQNZ010000000
VERSION     DAOQNZ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4377_length_6698_cov_3.874906"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..897)
                     /gene="xpt"
                     /locus_tag="PKW73_00665"
     CDS             complement(316..897)
                     /gene="xpt"
                     /locus_tag="PKW73_00665"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNA71819.1"
                     /translation="MQRLKDKILSEGKYLGKGILKVDSFMNHQIDAHLMKEVGEEFAN
                     RFKGLGTTRILTAETSGIAPALSAALTLDVPLVFARKNKPVTMADNPYKEAAQSHTHG
                     KMVELIVSPEYLSSSDRVLILDDFLASAKTIKALVKLVQASGATLVGIGAVIEKAFEG
                     GRQALVELGVKDIPVESLVAIKNFDGDRVNFVD"
     gene            complement(878..2356)
                     /locus_tag="PKW73_00670"
     CDS             complement(878..2356)
                     /locus_tag="PKW73_00670"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA71820.1"
                     /translation="MAEQKSQARGIDKLKGLPATMAFILVFFYALELSFSGWQLFKGV
                     DGDLLIKWGSNFGPNTLLEREYWRLFSYGLLHSSSVHMAVNFYALIEFGPLALAALGR
                     TKFIFIYILSGVIGGLTSILFNPTGNSVGASASIYGIVAAFIVVSWLRREKAVAGQRL
                     RKPELIMLLVFLLYSFLLGFTSDFMDNSAHIGGFLSGALAALILTIKHRSQGLSFRFN
                     LAAALALASIAPVMAEIDTRRMENNPKVDNYLLRSEGKRLVKEKKYLHGIDKLNEALK
                     ILPDDTNTLLARSEALLELDLFQAALKDLNQILQRDKTNKGALARRAIVYHNLNQWEN
                     SVADLDQAIKLDSGQALLYNNRAWMRLPAYKEGDNLNLAMADAEKAIKLSPHLFTAYD
                     TRATINIMKGDYEKATKDLVECLKQDDCRAAANFHLAIIDFVKGNLEEGEKKLALANK
                     LNYQPDQFELNFCRQELGLKGLSNTRLTEENTEGEKSAKTER"
     gene            2498..2929
                     /locus_tag="PKW73_00675"
     CDS             2498..2929
                     /locus_tag="PKW73_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA71821.1"
                     /translation="MNILVALDGSESSQRAIEFIQQRPWLDLDHFLIISVVEPDKADV
                     SMASALTKTSEAFFGECEKVLSRARDSVKEKLPRNKIETRILTGPVKDTIIDCAREFN
                     ADLIVMGSQGRKGINRLIIGSVAEAVLRGAPCSVQIIRKKG"
     gene            2960..3400
                     /locus_tag="PKW73_00680"
     CDS             2960..3400
                     /locus_tag="PKW73_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA71822.1"
                     /translation="MRVMIALDGSECSKYALDHVVKRPWRVDDLFLIAHVVEPIPVDI
                     GIAYVPNAYSQTDGAAADAAYALMEVAKKELHEALPSHSVETKVAFGMVKSELLDLAK
                     SWKADLIVMGSHGRTGFNRLLLGSVAEEVLRGAHCSVEIVKQHK"
     gene            complement(3583..4047)
                     /locus_tag="PKW73_00685"
     CDS             complement(3583..4047)
                     /locus_tag="PKW73_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA71823.1"
                     /translation="MNTLESNCSSQGALDKDDAKLIDFGLSVLSPRWTSEILIDLATG
                     PKRTTQLLKDLKGVSAKTLCQRLRKLTELGLVSRQTFAEVPPRVEYSLTSSGKELTSI
                     LNALKVLGADLYKNCKTRKRPLQNMEVEAETESAISAEFTAQTDMGQAAYSI"
     gene            complement(4231..4479)
                     /locus_tag="PKW73_00690"
     CDS             complement(4231..4479)
                     /locus_tag="PKW73_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71824.1"
                     /translation="MLDRKRETEFDLYLQAGQRLLEQGDLSQAEKILKSGLRKLDEHI
                     IEQNQLRSYFLKSLEVLKSSTACIQDSASDSISSKREY"
     gene            complement(4732..6351)
                     /locus_tag="PKW73_00695"
     CDS             complement(4732..6351)
                     /locus_tag="PKW73_00695"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HNA71825.1"
                     /translation="MNKRETHYMYCDALQEAGDEYPDLVEVEKRMNFLRFSPEDEARI
                     KAADALLEPHLAEMMDHLYSHFLANSETAAFFADSTVLERAKAAQLNYFSRLTKGDYA
                     VDYVRDRWCIGSTHHRINLDPKWYLGAYCGALLYMKDLLRGKLDSADYAETVSSLTKL
                     IFFDMSFAIETYILAKEKALREKQLSIQLLETEKKVTKSILESAPVGIIRLNRDLLVE
                     EVNEEFVELSNFKNRSEIIGKSFEEVCPGLSSKRFKLSIETGQTYQVQAEACVFGEKD
                     SRFFDWAVWPVTSELDSGPGLVAMFANITDRVRLQEQREDFVATLTHDLKTPILAANR
                     ALKLLMEGDFGEVTDGQKQMLDSILQSNDALYNLVLTLLDVYRYDSGAKQLSIGPFDL
                     SEIAERVVQELDPLARSKSITLSFVKPKQMQMVLLDPEEIRRVIQNLVDNSLKYSSSG
                     ASVTVRVDYREAEAILEVEDTGKGISDEDKPKLFQRFWQASPGRRYYASTGLGLYLCK
                     RIVDSHSGRIWCESTQGKGSKFSFALPLMEF"
BASE COUNT         1566 a         1594 c         1713 g         1825 t
ORIGIN      
        1 actaaactca tgtaaaggaa gaaattaaat ctgattgact cctgaaagcc ttatgtagca
       61 aggcttttat ttgctcaaaa gtcccgagaa cgtgatgtaa tatttcgcaa tttcactatt
      121 gttacatttg atcgcgtttt tctgagagac ccccggtctc cagtgcaaga tgaatagaac
      181 atttctcctt ctcttttgag ctggcatctt cgcgctcgcc tcgacggctt tcgtcgaagg
      241 agctttcgtt gaaggagctt tcgttgaagg tgctttcgtc gtaagagcta ccgcttcgct
      301 tgggtttcga agcttctaat ctacgaagtt gacccggtct ccgtcaaaat tcttgatggc
      361 caccagagac tctacgggaa tgtctttcac tccgagttct acaagggctt gtcgcccacc
      421 ttcaaaggcc ttttcaatga cggcaccgat tccaacaagc gtggcaccgc ttgcctgcac
      481 cagtttgacc agggctttga tggttttggc tgaggccaga aaatcatcga gaatcaagac
      541 gcgatcagaa cttgagagat actcaggaga gactatcagt tctaccattt tgccatgggt
      601 atgagattgg gcggcttcct tgtacgggtt gtcggccatg gtcaccggtt tgtttttgcg
      661 ggcgaaaacc agaggaacat caagggtgag ggccgccgat agggccggtg ctatacctga
      721 agtttcggcg gtgagtatgc gggtagtgcc aagacctttg aagcgattgg caaattcttc
      781 gccgacttcc ttcatgaggt gtgcgtcgat ctgatgattc atgaaagaat cgactttcag
      841 tatgccttta cccaggtact tcccttcgct gagaatttta tctttcagtc tttgcacttt
      901 tttctccttc tgtgttttct tctgtaaggc gtgtgttcga cagtcctttt aaccccagtt
      961 cctgtctgca aaagttcagc tcaaactgat ccggctgata gttcagcttg tttgccaggg
     1021 caagtttctt ttctccttct tctaggttgc cctttacaaa atcaattatg gctaaatgga
     1081 aattggcggc ggctcggcaa tcatcttgct tcaaacattc cactagatct ttggtggctt
     1141 tttcatagtc gcctttcatg atgtttatag tggctctggt gtcataggcg gtgaaaaggt
     1201 ggggcgagag tttgattgct ttttctgcat cggccatggc caggttgaga ttgtcacctt
     1261 ccttataggc aggcaaccgc atccaggcgc gattgttgta gagcagtgcc tgtccgctat
     1321 caagcttgat ggcctggtct aagtctgcca ccgagttttc ccactgattc aagttgtgat
     1381 aaacgatggc ccgtctggcc agggcgcctt tgttcgtttt gtctctctgc aaaatctgat
     1441 tgagatcctt gagcgctgct tgaaaaagat ccagctccag aagggcttca gatctagcta
     1501 gaagagtatt cgtatcatca ggcaagatct tcagtgcttc attcaactta tctatgccgt
     1561 gcagatactt tttctctttt accaggcgtt tcccctcgct gcgcagcagg taattgtcta
     1621 ccttgggatt gttttccatg cgcctggtat cgatttccgc catcaccggt gcaatagaag
     1681 cgagagccag ggctgcggcc aggttgaagc ggaagctaag accctggctg cgatgcttga
     1741 tggtgagaat tagcgcagcc agggctccgc tgaggaaacc gcctatgtga gcggaattgt
     1801 ccatgaagtc gctggtgaag cccagtaaga aactatagag caggaagacc aggagcatga
     1861 ttagctctgg ttttctaagc ctttgcccgg ctacggcttt ttctcttctc agccaggaaa
     1921 ctactataaa agcagctacg atgccataga ttgaggccga ggctcccacg gagttgccgg
     1981 tgggattgaa gagaatgctc gtcaatcctc cgattacacc gctgaggatg tagatgaaaa
     2041 tgaatttggt gcgcccaagg gcggccaggg caagcggtcc gaactcgatg agggcataga
     2101 aattcaccgc catatgcaca ctgctggagt gcaaaagacc gtagctaaac agtcgccagt
     2161 attctctttc caggagcgtg tttggtccga aattggaacc ccacttgatg agcaggtctc
     2221 catccacgcc cttgaagagc tgccagcctg agaaagagag ttccagggcg taaaagaaca
     2281 ccagaataaa agccatggtt gccgggagcc cctttagctt atcaattccg cgtgcctgac
     2341 tcttttgctc tgccattaac tgcttccctc agctagataa tacatgcgcc tcggcaattt
     2401 agcagttgct caccttcgca atatccccat tgcagacgaa gaggctgtag aattcttagc
     2461 attgtctagg aaatggattt agttaaggga agtcaatatg aatatcctgg ttgcccttga
     2521 tggctcggag agctcccagc gggctattga atttatccag cagagaccct ggcttgattt
     2581 agaccatttt ctgatcatca gcgttgtcga accggacaag gccgatgtga gtatggcctc
     2641 ggctcttacc aagacctctg aggcattttt tggcgaatgc gagaaagtgt tgagtcgtgc
     2701 tcgcgattca gttaaagaga agctgccaag gaataaaatc gaaaccagaa ttcttacggg
     2761 acctgtcaag gatacgatta ttgattgcgc cagagaattc aacgcagatc tgattgtaat
     2821 gggttcacag ggtcgtaaag gtatcaatcg cttgatcatt ggaagtgttg cggaagcggt
     2881 tttgcgcggg gcaccgtgtt cggtgcagat catcaggaaa aaaggttagt cagaattcag
     2941 tcaaaacagg gataagacaa tgcgagtaat gattgctctg gacggttcag agtgttccaa
     3001 atatgccctc gatcatgtgg tgaagcgccc ctggcgtgta gatgatttgt ttttgattgc
     3061 gcatgtggtg gagcccattc cggtggatat aggcatagct tatgttccca atgcctatag
     3121 tcagaccgat ggcgctgcgg ccgacgctgc ctatgctctc atggaagtgg ccaagaagga
     3181 gttgcacgaa gccctaccct ctcacagcgt agagacgaaa gtagccttcg gcatggtgaa
     3241 atcggaactc ttggatctgg caaagagttg gaaggctgat ctgattgtca tgggatccca
     3301 cggtcgtacc ggctttaacc ggctcttgct gggctctgtg gcggaggaag tgctgagagg
     3361 cgcacactgc tcagtagaaa tagtcaaaca gcataagtaa ttgccatttg gacggtagaa
     3421 atagtctacc tacatatgtg atgttccatt agttcaagcc caaagggata caaaaagaga
     3481 gccgctaccc ttggggtaac ggctctcttt atcagcggaa aatttcattt caagtccgag
     3541 cgttttggtt gatcggattt gacctgagcc gggctagccc gactagattg aataggcggc
     3601 ctgtcccatg tctgtctgag ccgtaaattc ggcggagatt gcagactcgg tctcggcttc
     3661 cacttccatg ttttgcagtg gacgctttct agttttgcaa tttttgtaga ggtcggcgcc
     3721 cagtactttc agcgcgttca aaatgctggt tagctccttg ccgctgctgg tgagcgagta
     3781 ctctacccgt ggcggtactt ccgcaaatgt ctgtctggat accagcccca gttcagtcag
     3841 ctttctcaat ctctggcaga gggttttggc actcactcct ttcaggtctt tcaatagctg
     3901 ggtggtacgc ttgggtccgg ttgccaggtc gataagaatc tctgaagtcc aacgcggact
     3961 gagtactgaa agtccgaagt caattagctt ggcatcgtct ttatcaagcg cgccctggga
     4021 agagcaatta ctttctaatg tgttcataag acttcaatag aactcctgtc ctgaatgttt
     4081 gactgtttgc tggcgcaaga aaagccactc tcctaatact ctcctgatct ggactaattg
     4141 ccatcatgca aaataatgaa actgccgacg gctaatcatc cttgcgaagt agcggctgca
     4201 agagaaattg ctggtattag accgactgtt ctaatactcg cgcttcgaac tgatggaatc
     4261 agaggcacta tcctggatgc aggcggtgct gcttttcagg acctcaagac ttttcaaaaa
     4321 gtagctacgt agctgattct gttcaataat atgttcgtcg agtttgcgta gcccactctt
     4381 caatatcttc tcagcttgac tcagatctcc ctgctcaagg agcctttgtc cggcctgaag
     4441 atagaggtcg aattcagttt cgcgctttct gtctaacatc gcttttcggt ctcatctcaa
     4501 gcaaacccac cagaacatca tccgataatg gcgggttgct aacatcaatc caaatggtta
     4561 aacctggatg gtcctgaggc tgatttcagt cgaagggatg attgaataat ggtaagtatt
     4621 ggaagtttag ccttgtctaa ggtgagcata gaggagcttt gttgccgttt atccgggagg
     4681 agcggctaac actccataag aggcagcgct agaactccgt caaaggaacg gctagaactc
     4741 catcagaggc agcgcaaagc tgaatttaga gcccttgccc tgagtacttt cacaccagat
     4801 tcgtccgctg tggctgtcca ctatgcgctt gcagaggtaa agccccagac cggtgcttgc
     4861 atagtagcgg cgaccgggcg aagcttgcca gaaacgttga aaaagcttgg gcttatcctc
     4921 gtcgctgata cccttgccgg tgtcttctac ttccagaatg gcttcggctt ccctgtagtc
     4981 tacgcgcacc gttaccgatg ccccactgct ggaatatttg agcgaattgt cgacgagatt
     5041 ctggatcacc cgcctgatct cctccgggtc tagcagcacc atctgcatct gtttgggttt
     5101 aacgaaagag agggtaatcg atttgcttct agccagcggg tccagttctt gcaccacgcg
     5161 ctcggcgatt tccgaaaggt caaagggccc tattgagagc tgtttggctc cggagtcgta
     5221 gcggtaaaca tccagcaagg tgaggacaag attgtagagg gcatcgttgc tctgaagaat
     5281 cgagtccagc atttgcttct gcccgtctgt aacttcgccg aaatcacctt ccataagcag
     5341 tttaagggcc cgattggctg ccagaatcgg tgttttcaag tcatgggtga gggtggctac
     5401 gaagtcttca cgttgttctt gcaagcgcac gcggtcggta atgttggcga acatggctac
     5461 cagacctggt ccgctgtcca gttcggaagt gacaggccag actgcccagt caaagaagcg
     5521 gctgtctttt tcgccgaaga cgcaggcttc agcctgcacc tggtaggtct gacctgtttc
     5581 tatggaaagt ttaaagcgtt tgctggacag gccgggacag acttcctcga agcttttgcc
     5641 aatgatttca gatctgtttt tgaaatttga tagctctacg aattcttcat ttacctcttc
     5701 taccagcaga tcgcggttca gacgtataat ccctaccgga gccgattcta gaatgctctt
     5761 tgtgactttc ttctccgtct ccaaaagttg aatcgataat tgcttttctc gcaaagcctt
     5821 ttctttagcc agaatataag tctcaatggc gaaagacata tcgaagaaaa tcagtttggt
     5881 aagggagctt accgtttcgg catagtccgc cgaatccaat ttcccccgga gcagatcttt
     5941 catgtacaaa agcgcaccac agtaggctcc caggtaccat ttaggatcta agttgatacg
     6001 atggtgggtg ctgccgatgc accacctgtc tcgcacatag tccaccgcgt aatcgccctt
     6061 agtgaggcga ctgaaataat tgagttgggc tgccttggct cgctccagaa ccgtgctgtc
     6121 tgcaaagaag gcggctgtct cggaattggc tagaaagtgg ctgtagagat gatccatcat
     6181 ctctgccagg tgaggctcca ataacgcgtc agctgctttt atccgggctt catcttcagg
     6241 gctgaagcgc agaaagttca tgcgcttttc cacttccacc aggtccggat attcgtcccc
     6301 agcctcctgc agggcgtcgc agtacatgta gtgggtttct ctcttattca attctgttga
     6361 agtctcttga agtgtcactc ttgctgtaat agctctggca tagtgtacta tgttaatctt
     6421 tctagtctaa cctgatactt tatgccggtg gtaatgtgag cgaagttgca aacgtgaaaa
     6481 tcaaagttct tctcgcggaa gaccatgctt taacgagaat tggcttaaag acggttttgt
     6541 caagaacaag cgatattgcc gttatcgggg aagccgagaa cggtcaggat gccatcgata
     6601 aggtggaaga actgaagccc gatgtggttt tgatggatgt gggcatgccg gtcatggacg
     6661 gcatcgaggc cgctggtaaa cttcgcctct ctcatccg
//
LOCUS       DAOQNZ010000021         6684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4391_length_6684_cov_4.102127, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000021 DAOQNZ010000000
VERSION     DAOQNZ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4391_length_6684_cov_4.102127"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PKW73_00700"
     CDS             <1..871
                     /locus_tag="PKW73_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71826.1"
                     /translation="FELKGERLKGGDFDLMVSSAQVVTALILAGLSADSPVTVSTPYV
                     VRDHTLRALRFMGVDIGCSHAVKQTATALMVRPLKGDLAPCRFSIPGDISSCAFFLVA
                     AAILPGSAVTLLNVGVNPGRTLVIEVLRRMGVQIDFLNEREVCGEPVCDLFVQAPAAL
                     TATDITAEEVPSGIDEIPALSLAFAFAAGRSTVRGASELKQKESNRLKLLTDNLQEAG
                     VAVEVYDDGFAVQGKGYLAGGGFWRTDLDHRLAMIGMVASLAARQPLMLEEKESCAVS
                     YPQFAADLKSLLV"
     gene            927..2096
                     /locus_tag="PKW73_00705"
     CDS             927..2096
                     /locus_tag="PKW73_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71827.1"
                     /translation="MGRAAVIFLATQLALLTFLAGRFPQAVLAQPGIEPGERSVQALS
                     LRKEVVARVDSKVKTVIAAGLPQYLGKARDYDGIFAQLAAAGVKVFLADGQCRQLPEP
                     ASLGYEADFYPTAQTISAPGTRKAYGAMKKHGVKLLVPADILYPLGQELPSPAEDPLL
                     RLIKTFGADAIYGVYAYDEPAKNADFKQMDKRCQAVYRRVKELSAALPVVMVHAPLVD
                     GFAAGDAQYLKRVLQLSRYADIVGFDVYPIPIHVAKLVDPFLTAAPAAARKAVPGVDP
                     LTGLFTDYRVAVAAYCRFLKKNLPGKDYLVVLQAFSYKDQGFDPVLAHLYGDRRPTGE
                     EMRTMVKVARKEGVGNIAWWGQGMVGGDPSAARKYWNEVLEASGSLDFPGHAGGK"
     gene            2126..4183
                     /locus_tag="PKW73_00710"
     CDS             2126..4183
                     /locus_tag="PKW73_00710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA71828.1"
                     /translation="MLAGQILDDTYEVLRTLGCGGMGEVYEVRHLVLQKKMALKTLRA
                     DAANPDNWQRFVHEARILARLHHPAIVEIYDMAVTPDGQPFYTMELLRGLSLQDYLLK
                     RGPLPEALALSLFKPVLSALACAHEAGVIHRDIKPHNIMLLGEAEAFADPLLASVRVK
                     VVDFGLAKLRGGNCQQSLTATGAVFGSPYYMSPEQCLGRRVDERSDLYSVGCALFQTL
                     TGAVPISGASSTDTMRAKVTQPSPRLSEVSTLKFSRLTEELVAVLLATDAEERFDSAR
                     QVLLSLDDHGEKSAGRINLFNAANALSEGSHSLAPQKGRRQRLLMLGVGALGLPLLLL
                     LASVCLSSGPMKSALPASAPVSGSVATATVASSSPSWFSTVDQSGGRVFDFSSLGTGL
                     ANPPKGLAWQGKTEEEMRKRWMASELGEMEELVNGGDGRILDLRHAVYVPPQADLRFR
                     PGLPFYRQPSLLSRFRLDEIHSFDFRPAAGDRKRSMSDILPQLLPFTRLRELIFRDTQ
                     LSAADLHDMGLLKNLTCLSLLTVDGITGEDLGRENLLLGLKQLRLDGLRDLSRVFEKL
                     SHSKRIETLEIHTSNLSDADIDKLVLVKNLRDLRIRNCDLTDAQLGRFSKLPQLCKLE
                     VRRNAITVDGLRQHLPEILSKRSAAASQNRLEVILSADFCTPSEKEDFKKAFPSTIFK
                     YVP"
     gene            complement(4194..4991)
                     /locus_tag="PKW73_00715"
     CDS             complement(4194..4991)
                     /locus_tag="PKW73_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71829.1"
                     /translation="MTILVAVKKGGRVFLGADRMMTSGSEYAVDVVNGSKIIKLKHAY
                     IATSGYTLLDNCIEHLYRTRHAMMENKFSNRHEVFQFFLDLFGELKKNYTLVDTGKDT
                     FAAIYNVFLVATPTSIYSISQNLSVTEYGNFAARGAGSDFAKGCLYGIYDLVDDGLTI
                     TRMALEASCHFSIYCKEPIDIIEVKAEDFGKHSPEGPKSRVKLVTHKERRGLQGMVLV
                     KEGSTDKARAKAGEASAANKGKGSAAKAEKKGSLSSLKKSTGKSRSK"
     gene            5189..5782
                     /locus_tag="PKW73_00720"
     CDS             5189..5782
                     /locus_tag="PKW73_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71830.1"
                     /translation="MVRFLSLLALPCLLVIGAASPSIAQGYLPPGQEMASGYQTQGRA
                     QTDPALIKRWFAAYDQVRRQAQMTPSERRQADAMLSKGLSMFVPGDEKLAGQKLLSGL
                     IGKYQQAMGQMKQLPLYPETEKLHRGYYQYFSDARNLFSDYIRVQNEPFAKDPNTGER
                     LIGQIVVRKANLTDLESNVKALDAQLRGGFGIAPYRY"
     gene            complement(5810..>6684)
                     /locus_tag="PKW73_00725"
     CDS             complement(5810..>6684)
                     /locus_tag="PKW73_00725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNA71831.1"
                     /translation="PAYIPPVDQDQPLAQPTVPAPYQNQGFTPLPPPTSSAPLAAETL
                     TEERIARVKELLQTAQLPRIIPTENTTPPHFFRILPPSQTEPVTSDYLENVLTASKNR
                     TFRFAAMPITVYITPIQDQSLMKAVREGFFDWESRGARVKFAETTNMAGARIKVIWSR
                     LGVSSDINDCTLGAHTITKWTKRPSGKLAVIPISAIPVPIYIPSLGPKYTVPPQVIEV
                     NLDLIYSKVPEARYLLTKNIIAHELGHAMGLLGHSPEKGDLMYAITDEHSRLSQRDLN
                     TLERLYARKVDVPL"
BASE COUNT         1317 a         1743 c         1935 g         1689 t
ORIGIN      
        1 atttgagctg aagggcgaaa gactcaaggg tggagacttt gacctgatgg tcagctcggc
       61 tcaggtggta acggctttga ttttagccgg gctctcggca gactcgccgg tgactgtgtc
      121 caccccttac gtggtgagag atcatacttt gcgggccctg cgcttcatgg gcgtggacat
      181 tggttgctct catgccgtta agcagaccgc aaccgctctc atggtccgcc ccctcaaagg
      241 tgatctggct ccctgtcgat tttccattcc cggagatatt tcttcctgcg ccttcttcct
      301 tgtggctgcg gccattttgc ccggcagtgc agtgactctt ctgaatgtcg gcgtcaaccc
      361 cggacgcacc ctggttatcg aggtgctcag acgcatgggt gtgcagattg atttcctgaa
      421 tgagagggaa gtttgcggtg aaccggtttg cgatttgttc gtgcaggcgc cggctgcttt
      481 gactgccacg gacattaccg ctgaagaagt tcccagtgga attgatgaga taccggccct
      541 ctctctggcc tttgcctttg ccgccgggcg cagtaccgtc cgtggtgcaa gcgaattgaa
      601 gcagaaagag tccaatcgcc tcaaactgct caccgataac ttgcaggagg ccggtgtggc
      661 tgtggaagtg tatgacgatg gttttgccgt gcagggtaag ggctatcttg ccggtggtgg
      721 gttctggcgt accgatctcg atcatcgcct ggccatgatt ggcatggtgg cgtcactggc
      781 ggctcgtcag cctctgatgc tggaggagaa agaatcttgc gccgtcagtt atcctcagtt
      841 tgcggcggac ctgaagtcgt tgctggtctg agaggcggtc gtacctggga tttttacttg
      901 ttcgtcaggg agcttaattg atattgatgg gccgggcagc tgtaattttc cttgcgacgc
      961 aacttgccct gctgaccttc ctggccggtc ggttcccgca ggcggtgctg gcgcagcctg
     1021 gcatcgagcc cggggagcgg tcggtgcaag cgctctcttt gagaaaggag gtggtagcaa
     1081 gggtggacag caaggtgaag accgtcatag ctgccggttt acctcagtat cttggtaagg
     1141 cgcgagacta tgatggcatt tttgcccaac tggcggcggc cggcgtcaag gtctttcttg
     1201 ccgacggaca gtgccggcaa ttaccggagc ccgcatctct aggttacgaa gccgacttct
     1261 accctaccgc tcagactatt tccgcgccgg gcaccaggaa ggcctatggg gccatgaaga
     1321 agcacggggt aaagctgctt gtgcccgccg acattcttta tccgctcggg caagaactgc
     1381 cgagcccggc ggaagatccc ttgcttcggc tgattaagac tttcggggcg gatgccatat
     1441 atggtgttta tgcctatgac gagccggcta agaacgccga ttttaagcaa atggataaaa
     1501 gatgccaggc tgtttaccgg cgtgtcaaag agttgagcgc cgcactgccg gtggtgatgg
     1561 tgcatgctcc cctggtagac gggtttgccg caggcgatgc tcagtatctc aagcgtgtct
     1621 tgcaactgag ccgctatgcc gatatcgtcg gcttcgacgt ctatccgatt cctattcatg
     1681 tggccaagct ggtggatcct ttcctgactg cagcaccggc ggccgctaga aaagccgtgc
     1741 ccggggttga tccgctcaca ggactattca ctgactatcg agtcgccgtt gccgcctact
     1801 gccgctttct caaaaagaac ttgcccggca aagactacct tgtggtgctg caggccttct
     1861 cttacaaaga ccagggtttt gatccggtcc tggcgcattt atacggcgat cggcgtccca
     1921 ccggcgagga aatgcggacc atggtgaaag tggcccggaa agagggtgtg ggcaacattg
     1981 cctggtgggg gcagggtatg gttggcggcg atccgtcagc cgcccgtaaa tactggaatg
     2041 aagtgctgga agcctcgggc agcctcgatt tccccgggca tgccggtggt aaatagttag
     2101 cagccggtga ggagaattgg gcaaggtgtt ggcggggcag atcttagatg atacctatga
     2161 ggtgctcagg accctcggct gcggcggcat gggtgaagtc tatgaagtgc gccatcttgt
     2221 cttgcagaag aagatggcct tgaagacttt gagggcggat gcggccaacc ccgacaactg
     2281 gcagcgtttt gtacatgaag cccgcatttt agcccgtctt catcatccgg ctatcgttga
     2341 gatttacgac atggccgtca ccccggacgg gcagccgttt tacactatgg aactgctgcg
     2401 tggcttgtcg ctgcaggact acctgctcaa gcggggaccg ctcccggaag cgctggcgct
     2461 ttccctgttc aaacctgtgt tgtcggcgct agcctgtgcc catgaagccg gtgttatcca
     2521 ccgggacatt aagcctcata acataatgct cttgggagag gcggaagctt ttgccgatcc
     2581 gctgttggca tcggtgcggg tcaaggtggt tgattttggt ctggccaagt tgcgaggcgg
     2641 caattgccag cagtcgctta ctgccacggg ggcggtcttc ggcagtccgt attatatgag
     2701 tccggagcaa tgcctgggtc gcagggttga tgagcgaagc gacctgtatt ccgtcggttg
     2761 tgctctcttt caaactctta ccggggcggt gcctatttca ggagcttcca gcaccgatac
     2821 catgagggcc aaggtgacgc agccttcacc gcgtctgagc gaggtttcca ccctcaaatt
     2881 ctccagactg acggaagaac ttgtggctgt cttgctggcc actgatgccg aagagcgttt
     2941 cgatagcgcc aggcaggttc tgctcagtct tgatgaccat ggagagaagt ccgccggcag
     3001 gatcaatcta ttcaacgctg ccaacgccct ttctgaaggc tctcactccc ttgctcccca
     3061 gaaaggcagg cggcaacggc tgcttatgct cggtgtggga gccctcggtc ttcctcttct
     3121 acttcttctt gcttcggtct gtctgtcttc aggtccgatg aaatctgccc ttccggcaag
     3181 cgccccggtc tcaggctcgg tggccacggc gaccgtcgcc tcaagttctc cctcctggtt
     3241 ctctaccgtg gatcaatccg gggggcgcgt cttcgacttc tcctcccttg gtacggggct
     3301 tgccaatccg cctaaaggct tagcctggca agggaagacg gaagaggaga tgagaaagag
     3361 gtggatggcc agtgaacttg gcgaaatgga ggaactggtc aacggtgggg atgggcgtat
     3421 tttggatttg aggcatgcgg tttatgtgcc tccgcaagcg gatttgcgct ttcgaccagg
     3481 cctgccattc tatcgtcagc caagtctgct ttcgcgtttc cgtcttgatg aaattcactc
     3541 ctttgatttt cgtccggcag cgggggaccg caaacgcagc atgtctgata tcctgccgca
     3601 actattgcct ttcaccaggc tgagagagct tatctttcgc gatacgcagt tgagcgccgc
     3661 tgacctgcac gatatgggct tgctgaagaa tcttacctgc ttaagcctgt taaccgtcga
     3721 tggtatcact ggcgaagacc tgggtagaga gaatctgctt cttgggctga agcagttgcg
     3781 gttggatggt ttgagagacc tttcccgcgt atttgaaaag ctcagtcact cgaagcgcat
     3841 cgagactctg gagattcaca ccagtaacct ttccgatgcc gacatcgaca aacttgttct
     3901 ggtaaagaac ttgcgcgacc tgcgcattcg caactgcgat ttgaccgatg ctcagttggg
     3961 ccggttctcc aagcttcccc aactatgcaa gctggaagtc aggcgtaatg ctattacggt
     4021 tgatggactc aggcaacacc tgccggagat tctaagcaaa agaagcgccg ccgcctctca
     4081 aaaccgactg gaagttatcc tgtccgctga tttttgtacg ccttccgaga aggaagattt
     4141 taagaaagcc ttcccgtcca ccatcttcaa gtatgtcccc tgatttgaaa gagctacttg
     4201 cttcgcgact tgccggtgct ctttttcagg ctggagaggc tgcctttctt ttctgcctta
     4261 gcggcagacc ctttaccctt gtttgcggcg ctcgcctccc cggctttcgc cctggctttg
     4321 tcggtggagc cttccttcac cagcaccatg ccttgaagcc ctcgcctctc tttgtgggtg
     4381 accagcttga cccgagactt gggaccttcc ggggaatgtt tgccgaagtc ttctgctttt
     4441 acctcaataa tgtcgatggg ttccttgcag taaatagaga agtgacagga agcttccaga
     4501 gccattctgg ttatggtgag accgtcgtct accagatcgt agatgccgta aaggcagcct
     4561 ttggcaaagt ctgaaccggc tccgcgggct gcgaaatttc cgtattcggt gacggagagg
     4621 ttttgcgaaa tggaatagat gctcgtgggc gttgccacca gaaaaacgtt gtatatggcg
     4681 gcgaaggtgt ctttgccggt gtccaccagg gtgtagttct tcttcaactc tccgaacaaa
     4741 tccagaaaaa actgaaatac ttcgtggcga ttggagaatt tgttctccat catggcgtgt
     4801 ctggtgcgat aaagatgctc gatgcagttg tccagaagag tgtagccgga agtggctata
     4861 taagcatgtt tgagtttgat gattttggag ccgtttacta catctaccgc gtattcactg
     4921 cctgaggtca tcattctgtc ggcgccaagg aagacgcgac cgcccttttt tactgctacc
     4981 aggatagtca ttgatggggt ttccctctaa tttgtgctcg agactcctta ataagtaccc
     5041 gccggaaaca tttacctcac atttgcagtc ttgcctttgc attgggttgc tttcccctga
     5101 ggtttaatag ccccgggtga cttcctcaaa tttggggaat gcatttagaa taggtgtctt
     5161 ccggtagtcg gtttcaagag gattttccat ggttcgtttc ctcagcctct tagctcttcc
     5221 ctgtttgctc gtgattggag cggcttctcc ctcaattgcg cagggttatc ttcctcccgg
     5281 tcaggaaatg gcctctggtt atcagaccca gggtcgggcg cagaccgatc cggctttgat
     5341 caagcgctgg tttgccgctt acgaccaggt gcggcgccag gcacaaatga cccccagtga
     5401 acgcaggcag gccgatgcca tgctttccaa gggactttcc atgttcgttc ccggcgatga
     5461 gaaactggcc gggcagaagc tattgagcgg tttgataggc aaatatcagc aagccatggg
     5521 acagatgaaa cagttgcctc tctatcctga aaccgaaaaa ctgcaccggg gatactacca
     5581 gtacttcagc gatgccagaa atctcttctc cgactacatc agggtgcaaa acgaaccttt
     5641 tgccaaagac cccaataccg gtgaaagact tatcggacag attgtggtgc gcaaggccaa
     5701 tttgaccgac ctcgagtcaa atgtgaaagc tcttgatgct cagcttcggg gcggcttcgg
     5761 tattgctcct taccgctact agcccttccc tctctctctc aagctcaggc tagaggggca
     5821 catccacttt tctggcgtac agtctttcca gtgtattcag atctcgctgg ctgaggcggg
     5881 agtgctcgtc ggtaatggca tacatcaagt cgcccttttc cgggctgtgt cccagcaagc
     5941 ccatggcatg accgagttcg tgggcaatta tgtttttggt tagcagataa cgagcttccg
     6001 gtacctttga atagatcaag tccaggttca cctcaatcac ctggggcggc accgtatact
     6061 ttggtccaag gcttggaata tagatgggta ccggtatggc gctgatggga atgactgcca
     6121 gtttgcccga tgggcgcttg gtccatttgg taatggtatg tgctcccagg gtgcagtcgt
     6181 tgatatcgga gcttaccccc aggcgactcc agatcacctt gatgcgagca ccggccatat
     6241 ttgtggtttc agcaaacttg accctggcgc cccgactctc ccagtcgaag aagccttcgc
     6301 gcacggcttt cataagtgac tggtcttgaa taggagtgat gtaaacggtg atgggcatgg
     6361 cggcaaagcg aaaggtgcga ttcttgctgg cagtcagtac gttctccaga taatccgagg
     6421 taaccggttc ggtctggctg ggcggcagta tgcgaaagaa gtgcggcggt gtggtatttt
     6481 ccgttggtat gatgcgcggc agttgggctg tctggagaag ctcttttacc ctggcaatac
     6541 gctcttccgt aagggtttca gctgccagag gggctgagct ggtcggcggc ggcaatggcg
     6601 tgaaaccttg attctggtat ggcgccggta cagtcggttg agcgaggggc tgatcctgat
     6661 caactggtgg tatgtaggcg ggtt
//
LOCUS       DAOQNZ010000022         6597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4485_length_6597_cov_3.699480, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000022 DAOQNZ010000000
VERSION     DAOQNZ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4485_length_6597_cov_3.699480"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKW73_00730"
     CDS             complement(<1..369)
                     /locus_tag="PKW73_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71832.1"
                     /translation="MSWQKLEDLLKQVDRGGLSSLDRQGLTELGRLYRATSADLSRAR
                     ALKLNSDLQIYLNNLVVRAHNQVYQNRESRLSDILQFFLHGFPRQVQKNILYVLAAFL
                     IFILPCLGCYREALYDANFGQ"
     gene            471..1277
                     /locus_tag="PKW73_00735"
     CDS             471..1277
                     /locus_tag="PKW73_00735"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNA71833.1"
                     /translation="MQQPDYSISTPENVDLHLEIAGIGNRLLAQLIDTLIQAAIFAFI
                     AILAVAVGMAISVAPLDSKTKGIAYSILAMLAILCMFFMQNLYFIVWEGAWKGQTPGK
                     KLAEIRVIERTGQPIGWGASIIRNLLRVADGVFLLGLVVMLVDKNERRLGDMCAGTIV
                     IRERQAHITDSEIKVQEGLEADSTLDVGRISPADYDLLVDYLKRRGSLTEKARPKVAR
                     QIFLHLQEKLADKDSAPESENTEEKGPAAYSDFEKYLEKVYLSYQARAAQ"
     gene            complement(1384..2502)
                     /locus_tag="PKW73_00740"
     CDS             complement(1384..2502)
                     /locus_tag="PKW73_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNA71834.1"
                     /translation="MVKAGLEDIVAASSSVCDVNGTEGRLIYCGYDINDLAENSTFEE
                     VVYLLWYGRLPTKTELETLSTGLKTNRRIPSDVVKAMQHYPKDALPMEVLRTAVSMLS
                     MYDNESEDKGREPNIRKAIRLTAQIPTVVAYWDLIRNGKEVVAPKEDCSLSHNFLYML
                     SGGKEPDAVAVRSLDVALILHADHELNASTFAARVAAATETDIYSAIVAAIGTLKGPL
                     HGGANQEVIKMLLEIGSLDNVVPHLDKMFANHQKVMGFGHRVYKTTDPRASHLRRMSQ
                     ELGKGMGETKWFDMSAKIEEIVKADKGLNANVDFYSASVYYMLGIPTDLYTPIFAISR
                     MSGWAAHVLEQYANNRLIRPRAEYTGERNLKYVAIDKR"
     gene            2657..4765
                     /locus_tag="PKW73_00745"
     CDS             2657..4765
                     /locus_tag="PKW73_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNA71835.1"
                     /translation="MKTRSATSLLKRLARSLAALAEQDRQQAARFSAHLSLAAAGLCP
                     APLDRKLIETLNRLIHGPQAKLIYDASDFAGLTDSFHLSFASRKSEFWEQLGNLPGQL
                     QYPRQALGDPLFTGNLWEFLPPLKTPQSNSEFNLSNDPKGNLADTQFFTPLWIAADMA
                     ESLDLSGESTVLDPACGAGHLLVAVLEAALSQSLSRGEPVQAALEGLLKDRLGGFDVD
                     TDMLDLSGLALYMAARKHCWGELPRPNLQKLELPLGSLDRAFRHGHSRPGSPPTEIGS
                     FDNVIMNPPYQSSRTMDSITADYLKEHYPNSKNDLYAAFLELALALLKDEGRLTTITQ
                     QSFFTIQRFERLRLELLAGARLLSVKALGAGAFPTCPGEKVNTAILTLEKRKTKPASQ
                     EHLQTGKGNFNYCLYKRDKGGGTMALLKNVELQEGALLEVARAIPGFPFIFDAPPELP
                     ALFSNFTSLGQLEKEGELSIVNGLFTCNNKLFIKSKEELQPQEAALYVPYDKGGGRKW
                     YYDTPLRLNWHPNGEAIRAYRAGRGQSRALPGEAFYFQGGITYSYIGTSGFTARLLSA
                     GAVFDIASSAIFTRNIDKLTLLAYLNSATAIYLLAKLNPTINFQIGDLRRLPFKLPPP
                     ALDTSLQAQAAACIELMKEASHCSASGNLPRLQKILAEEARQQAEIDRLIFEHMGVGS
                     TLRREMLENDWVLSARAKVL"
     gene            complement(4752..5501)
                     /gene="sfsA"
                     /locus_tag="PKW73_00750"
     CDS             complement(4752..5501)
                     /gene="sfsA"
                     /locus_tag="PKW73_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HNA71836.1"
                     /translation="MLFRSLTEATLLRRYKRFLADVRLPCGTEVTAHCPNPGSMLGLA
                     QPGMRVLLWQAPPGEKARKLKWSWKFVEADGAMVCVDTMLANKLFKEAFTAGRLPEFS
                     GYSSIEAEHKYEDSRFDFFLQGTAGSAMVEVKSVSLLELSEQGEKLAAFPDARTERGL
                     KHLETLRSLNLKGADQAVQFYVVARGDTRAFKPADSIDPAYGRALRQAYAQGVAVHCR
                     AVEIKLEPCGEHCFDLEMTLAETLPLLLKEP"
     gene            complement(5522..6373)
                     /locus_tag="PKW73_00755"
     CDS             complement(5522..6373)
                     /locus_tag="PKW73_00755"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA71837.1"
                     /translation="MSVTFLPLTTKRGEASSGARETTGASAAALAAFRKLGTLVPART
                     ASPSVGLALSLALPLIIPFSAAAAAPEKASQAQAGAACGTLNQGGPAWNTVERLLRGF
                     SEDFALTRQKGKGAASAGHSQPSQIIDYQEMSVRALGRRQWETLSTSQKRDFSASLEA
                     LVEKRYYPRWRKIFGKGKVTFVEETGVGGDTLVKTRLTLGKKVETLAWRLTDKGTRVV
                     SLSVSDKDLLERLKSRINSRCKKGKLDVDQLIAWMRNSGGETQTSLSVEGASLAVSQG
                     STNVVAD"
     gene            complement(6400..>6597)
                     /locus_tag="PKW73_00760"
     CDS             complement(6400..>6597)
                     /locus_tag="PKW73_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71838.1"
                     /translation="PIKAMPYWEQKLAAIMPLYYACDLTKGAMLGIPCEAGRDVIVLL
                     GFAFISLFLSRTVLARSQASV"
BASE COUNT         1584 a         1937 c         1731 g         1345 t
ORIGIN      
        1 ctgcccgaaa ttggcatcat atagcgcctc ccggtaacag cccaggcaag gcaaaatgaa
       61 gatcaggaaa gcagccagca catacaaaat atttttctgc acctgccggg gaaaaccatg
      121 caggaagaac tgcaaaatgt cggaaaggcg actctcccgg ttctgataga cttggttgtg
      181 cgctctcacc accaggttgt tgaggtaaat ctgcaagtcg gaattgagtt tgagggcgcg
      241 ggcgcgagaa aggtcggcgg aggtggcacg atatagacgt cccaattcgg tgagcccttg
      301 ccggtccaaa ctggaaagcc cacctcggtc cacttgtttg agcaggtcct ccagcttttg
      361 ccatgacaac ctgcgagttc tcaaccagcg ctctacgttc atttaagctc gaatttcata
      421 gtcaagacaa aactctcttt taattcccaa agccataccc ggaaaagccg atgcaacagc
      481 cagattactc tatctctacc cccgaaaacg tggatctaca cctggagatt gccggcatcg
      541 gcaacaggct tttggctcag ctcatcgaca ccctcattca ggcagccatt tttgccttca
      601 tcgccattct ggccgtggcc gtcggtatgg ctatttcagt cgcccctctc gattccaaga
      661 ccaagggtat tgcctattcg attttagcca tgttggccat cctttgtatg tttttcatgc
      721 aaaacctcta ctttattgtc tgggagggcg cctggaaagg acagaccccc ggcaagaagc
      781 tggcagaaat aagagtgatc gagcgcaccg gccagcccat cggctggggc gcttccatca
      841 ttcgcaatct gcttcgcgtg gccgacggcg tcttcctgct tggtctggtg gtcatgctgg
      901 tagacaaaaa tgagcgccga ctgggagaca tgtgcgccgg taccatagtc attagagagc
      961 gccaggctca cataaccgat agcgagatca aggttcaaga aggactggaa gccgacagca
     1021 ccctggatgt agggcgcatc agcccggccg actacgacct tttggtggat tatcttaaac
     1081 gccggggcag cctgacggaa aaagccagac ccaaggtggc caggcaaatt ttcctgcacc
     1141 tgcaagagaa gctggccgac aaggattcgg ctccggaaag cgaaaacaca gaagagaagg
     1201 gccctgccgc ttacagtgat ttcgagaagt atctggagaa agtctacctc tcctaccagg
     1261 ccagagccgc ccagtaattt gacccgggcg agccaagccg aataataatg aaagagcccc
     1321 gctgcaaaga acggggctct ttcaatttac taccggcacc gacggcaccc tgaagacagg
     1381 gtactagcgc ttatcaatag ccacgtactt gaggtttctt tcaccggtat actcggcgcg
     1441 ggggcgaatc agcctgttgt tggcgtattg ctccagcaca tgggccgccc aaccggacat
     1501 gcggctgatg gcgaagatcg gcgtatagag gtcggtggga atacccaaca tgtagtagac
     1561 ggaagcggag tagaagtcga cgttggcgtt caaacctttg tcggctttga caatctcttc
     1621 aatcttggcg gacatatcga accacttggt ttcgcccatg cctttgccca attcctggga
     1681 catgcggcgc aggtgggaag ctctcgggtc ggtggttttg tagacccggt gaccgaagcc
     1741 cattactttc tggtggttgg cgaacatctt atcgagatgg ggcacaacgt tatccaggct
     1801 gccaatctcc agaagcattt tgattacttc ctggttggcg ccgccgtgca agggaccctt
     1861 caaggtgccg atggcagcca caatcgccga ataaatatcg gtttcagtgg cggcggcaac
     1921 ccgggccgca aaagtagaag cgttcagttc gtgatcggca tgaagaatca aggccacgtc
     1981 aagagaacga accgcaacgg catcgggctc tttaccgccg gaaagcatat aaaggaagtt
     2041 gtgggaaagg ctgcaatctt ccttgggcgc cactacttcc ttgccgtttc taatcaaatc
     2101 ccagtaagcg accacggtgg gaatctgagc ggtgaggcgg atggctttgc ggatattggg
     2161 ctcgcggcct ttgtcttccg actcgttgtc gtacatggaa agcatggaca ccgcggtgcg
     2221 caaaacttcc atggggagcg catccttagg ataatgctgc atggccttaa cgacatcgga
     2281 agggatgcgt ctattcgttt tcaaaccggt ggaaagagtc tccaactcgg tcttcgtggg
     2341 caagcgcccg taccacagca ggtagaccac ttcttcaaaa gtggaattct ctgctaaatc
     2401 attgatgtcg taaccacaat aaatcagacg tccttccgta ccattgacat cgcaaactga
     2461 ggacgacgcg gctactatat cttcaagccc tgccttgacc atgacctgat tccccaaact
     2521 aagtactgat gggaatttta tacaccaaat gacatcggtg gcggggcaaa taatagactt
     2581 atcttcatac tggtaaaaaa aatccagctc aatccggaca gtcagacttc ttgagagccc
     2641 cagcttgccc cctaacttga aaaccagatc agccacaagc ctcctcaaac ggctggccag
     2701 gagccttgcc gccctggccg agcaagaccg gcagcaagcg gcccgttttt cggcccatct
     2761 cagcctggcg gcagccggcc tctgcccggc tcccctggac agaaaactaa tcgagaccct
     2821 gaaccgcctc atacacggtc cccaggccaa actcatctat gacgcaagcg atttcgccgg
     2881 tctgacagac agtttccatc tgagcttcgc ttccaggaaa agtgaatttt gggagcagct
     2941 tggcaatctt cccgggcagc tgcaataccc ccggcaagcc ctgggcgacc ccctcttcac
     3001 cggcaactta tgggaatttc tgccgccgct gaaaacacca caatcgaatt ctgaattcaa
     3061 tctctcaaat gacccgaaag gcaatctggc ggacactcaa tttttcaccc ccctctggat
     3121 tgccgcggat atggccgaga gtctcgactt gagcggtgag agtaccgttc ttgacccggc
     3181 ctgcggcgcc ggtcatttac tggtggcagt attggaagcg gctctttctc aaagcctgag
     3241 ccggggcgaa cccgtgcaag ccgccctgga aggtctcctg aaagaccgcc ttggcggttt
     3301 cgatgtggac acagacatgc ttgacttaag cggtctggcg ctctacatgg cggcgcgcaa
     3361 acactgctgg ggcgaactac cccgccccaa cctgcaaaag ctagaactac cgctcggtag
     3421 tctcgaccgc gctttccggc atggccacag ccgccccggc agcccgccga cagaaattgg
     3481 cagcttcgac aatgtgataa tgaacccgcc ctatcaaagc agccgcacca tggatagtat
     3541 caccgccgac tacctgaagg aacactatcc aaacagcaag aacgacctct acgcggcctt
     3601 tctcgaattg gcgctggcgc tcctgaagga tgagggtcgt ctcaccacca tcacccaaca
     3661 aagcttcttc actattcagc gctttgagcg gctgcgcctg gaactgctcg ccggagcccg
     3721 ccttctcagc gtgaaagccc tgggggcagg cgcctttccc acctgcccgg gagagaaggt
     3781 gaacaccgcc attcttactc tggaaaagcg aaagacaaag cccgcctcgc aagaacatct
     3841 gcagaccggc aagggcaact tcaactattg cctctacaaa agggacaagg gcggcggcac
     3901 aatggcactg ctcaaaaacg ttgaactgca agaaggcgct ttactagaag tagcccgggc
     3961 tattcctggt ttcccattta tcttcgacgc accgcctgaa ttgcctgctc tcttcagcaa
     4021 cttcaccagt ctgggccagt tggagaagga aggcgagctg agcatagtga acggactatt
     4081 cacctgcaat aacaagctct ttatcaaatc gaaagaagag ttgcagcccc aggaagccgc
     4141 gctctatgta ccctatgaca aaggcggtgg gcgcaagtgg tactacgata caccgctcag
     4201 gctcaactgg catcccaacg gcgaagccat ccgcgcctac cgggccggcc ggggacaatc
     4261 acgagccctg cctggtgaag ccttctactt ccagggcggt atcacctact cttacatcgg
     4321 cacatccggt ttcacggcca ggctattgag cgcaggcgcc gtcttcgaca tcgccagctc
     4381 agccatcttc acaaggaaca tagacaagct aactctcctg gcctatctaa attccgccac
     4441 cgccatctac ttgctggcca agctcaaccc taccattaac ttccaaatag gcgacctgcg
     4501 ccgcctgccc ttcaaattac cgccgcccgc actggacaca agcctgcaag cgcaagccgc
     4561 tgcttgcatc gaactcatga aagaagcatc gcactgcagc gcctcaggca acctcccccg
     4621 tcttcaaaaa atactggcgg aagaagcccg gcaacaggcg gaaatagacc ggctcatatt
     4681 cgagcacatg ggtgtgggaa gcacactgcg ccgggagatg ctggaaaatg actgggtatt
     4741 gagcgcccgc gctaaggttc tttaagcaag agaggcaaag tctcagccag ggtcatttcc
     4801 aggtcgaaac agtgttcgcc gcaaggctca agcttgatct ccacggcccg gcaatgcacc
     4861 gccacgccct gggcataagc ctgacgcaaa gcccggccgt aggccgggtc tatactgtca
     4921 gccggcttga aggcccgggt gtcgcccctt gccactacat aaaattgcac ggcctggtcg
     4981 gcacccttca agttgagaga ccgcagggtc tccagatgct tgaggccgcg ctcggtgcga
     5041 gcatcgggaa aggcggccag cttctcacct tgctcggata attccagaag actgaccgat
     5101 tttacctcca ccatggcact accggcagtg ccctgcaaga aaaagtcaaa acgactgtct
     5161 tcgtacttgt gctccgcttc aatagatgaa tagccggaaa actcaggcaa gcgtccagcg
     5221 gtaaaggctt ccttgaatag tttattggcc agcattgtat caacgcagac catggcaccg
     5281 tcagcctcca caaacttcca ggaccacttg agtttgcgag ccttctcgcc gggcggcgcc
     5341 tgccatagca aaacccgcat cccgggctga gccagaccca gcatgctgcc ggggttaggg
     5401 cagtgagccg ttacttccgt gccgcagggt aagcgcacat cagccaggaa acgcttgtag
     5461 cgcctcagta aagtcgcttc cgtcaatgag cgaaacagca taagaactac taaaagcccg
     5521 gtcagtcggc cacaacattg gtggagccct gcgatacagc cagggaagca ccttccacag
     5581 aaagagaggt ctgcgtctcg ccgccggaat tacgcatcca ggcgataagc tggtctacat
     5641 ccagcttacc cttcttgcag cgggagttta ttctggactt caggcgttcc aacaagtctt
     5701 tgtcggagac ggataaactg accacccgcg tacctttatc cgtgaggcgc caggctaatg
     5761 tctccacctt cttgccgagg gtgagcctgg tcttgaccag ggtatcgccg cccacgccgg
     5821 tctcttccac aaaggtcacc ttgcccttac cgaagatttt gcgccagcgc ggatagtaac
     5881 gcttctccac gagagcttcc aggctggccg aaaaatcacg cttctggctc gtcgaaagag
     5941 tttcccactg gcgacgacca agagcccgca ccgacatctc ttgataatcg ataatctgag
     6001 aaggctgcga gtgcccggcg ctggccgcac ccttaccctt ctgcctggtg agggcgaaat
     6061 cttcgctgaa gccgcgcagc aggcgctcga cggtgttcca ggccggtccg ccctgattga
     6121 gggtaccgca ggccgcaccg gcttgagcct gggaagcttt ctcaggggca gcggccgctg
     6181 cactgaaagg aattatgaga ggcagagcca ggctcagggc cagcccaacg gacgggcttg
     6241 ccgtgcgcgc cggcaccaat gtgcccagtt tgcgaaaagc agcaagggca gcggctgaag
     6301 ctccggtagt ctcacgggct ccggaagagg cttctccccg ttttgttgtc aggggcaaaa
     6361 aagtcacact cactccgctt tcctcttggt taaccaagac tacaccgaag cctgactgcg
     6421 agcaagtact gttctcgaaa ggaaaagtga gataaatgca aagcccagaa gcacaatcac
     6481 atctctaccc gcctcacagg gtataccgag catggcacct ttagtgagat cgcaggcgta
     6541 ataaaggggc attatggccg ccagcttctg ttcccaatat ggcatggctt taatggg
//
LOCUS       DAOQNZ010000023         6538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4548_length_6538_cov_3.937683, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000023 DAOQNZ010000000
VERSION     DAOQNZ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4548_length_6538_cov_3.937683"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKW73_00765"
     CDS             complement(<1..346)
                     /locus_tag="PKW73_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNA71839.1"
                     /translation="MSEDNIDKKKGFWGTTLTRLKSVLAKTKEQVQDPEELAQGAETA
                     AAGAESQTAAAVQPEPIKAAAATSVATAHKPVDEEYIEALEERLIKADLGLPTVEPLV
                     EALRQEARGKNWT"
     gene            complement(354..1130)
                     /gene="nusB"
                     /locus_tag="PKW73_00770"
     CDS             complement(354..1130)
                     /gene="nusB"
                     /locus_tag="PKW73_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNA71840.1"
                     /translation="MSARRIARELAVIVMPQLPKNLDKLAETQIMELTDRAIHMLVDY
                     ARQNLTDAQALVIGAQDHLTQIETEHEDNKHFVEDLRAVPLTSSQFREKLQTIETAIA
                     LVSEALDIPEMALARGTETTRTNCKKCGAIVEATINKPHKEEVRDFVVRLISVYLEHK
                     ETIDEFIKRSKSKWRMERMVSIDRDILRLACAETFYMPDIPINVAISEAVELSHRFAD
                     ARAAKFINGILGDLSEEAEAVRRAAARKLAKEKAESISQN"
     gene            1216..1794
                     /gene="rsmD"
                     /locus_tag="PKW73_00775"
     CDS             1216..1794
                     /gene="rsmD"
                     /locus_tag="PKW73_00775"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNA71841.1"
                     /translation="MGISEEFGLRITGGELRGRSVTCPRGLAVRPTSSKVRQAFFNIL
                     GDRLRHARFLDLFSGSGLMGFEALSRGATVTFVERHNPSRAAIKATAEQFKLSEERYR
                     VRGGDVLKVLPELAGEEFDIVFADPPYKLCLGPAVAELLVRHRLLSAGALYVVEHMSD
                     DPVTLTDGSLERVDFRQYGGTAISMFQQVCTK"
     gene            1805..3169
                     /locus_tag="PKW73_00780"
     CDS             1805..3169
                     /locus_tag="PKW73_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71842.1"
                     /translation="MSQGELTGKETEKRDRREVGVDTGHGLSGVSYTASGINDALSMA
                     IGHVRLGDLLKASGIISYEFLKEALSSFEEKGLPLGKVLTLSGYLTASELAVALEIQT
                     LINNRQLPFDVGVRVLAISHDEKVPLSEAFSRASVVQPEDLLSNKLGQLLSQSRLISN
                     EDLDQALAVSQRTGLPLGHIFCYRNLLSQQLLDTALLGQQLVRRGSLSRENCLEALSA
                     AREREVALLSLPVNAAYKPSMRRGTSRLGELFFSAGILDDMSLISALQLSLSRGIPCG
                     EAISEVQGTSTAVIEQAVCMQEMLDNETLTLEQVKDVFIGIFHQERSFEHALAEASVA
                     GCSGNPARLMVQLLKDAQILVLPEDSSTTPAALAPVLTELTTCLDVNYNQGGVVGRLL
                     ISAGLVSASAAWAALRLTHLLTLEVVPYERAVAALEAANLQGSVDEVLIGFGALNRTR
                     LRWR"
     gene            complement(3175..5589)
                     /locus_tag="PKW73_00785"
     CDS             complement(3175..5589)
                     /locus_tag="PKW73_00785"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNA71843.1"
                     /translation="MSTGVDDSLKKAEDFPSPQALLGWVGAALSGFICLFLSFSTIHI
                     FSPGLQERDIADRDLFAPQAASVVDRAETEKAIEKARQSIIPVFQVDRSRDQESLKRI
                     ETVFARAESLQAMGIKPYHEASNAQKPNKARGKMKNRQPEAVPAPSSMDKKLQMSLLT
                     LPDDKLVGQAQANLPEIQTQRKAYIRARTAGLSGDLELELALTVDPAEMPAFKAATLE
                     SARRLMAKFHRYPVVDKGVWQDVVTEFLPDSLEAGKKQTAALVINENLEANLVIDTEG
                     TKTKAQVVVQGVEPVLKAIKEGEVIVPQGAMITREKLYILQELGITHVNRWPFILSMA
                     ISLIAALTLVGLFLYTYEPKHLFSPSSIGLMFTVQVIVASVASLVGKTNPQFVPIPAA
                     ALILTIFFGRRTAIALSLPMVLLIGVDRLVDLHYLAAHTLASLGAIMSFPQSGTGANG
                     GRSAVFRTGLVVAFGLVAGDLASTLVDHSYGGNLGIFGRRLLLDFAGGIISSIVAVGS
                     LPFLENLYGLITPFRLAELTDANQPLLRRLEENAPGTYQHSLAVANMAEAGAKAIYAD
                     ANMVRAGALYHDIGKMVRPKYFIENQLGATNPHDSMSPEDSRARVLAHVTDGIALAQK
                     YALPKAIQDFIPMHQGTTLMAYFYHKACTRDGADKVDANFYRYPGPKPNTKETAIVML
                     ADVSEAVTHSMRDPSQEEVEQALEKVFLNRWEDGQFSESGLSLEELHKVKAGFARVWR
                     TLHHERLKYPATTTGKMPIAPQVEVREEARVEARDEACDCAQAQEIPQAETVESVESA
                     APSSSN"
     gene            5757..>6538
                     /gene="galE"
                     /locus_tag="PKW73_00790"
     CDS             5757..>6538
                     /gene="galE"
                     /locus_tag="PKW73_00790"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNA71844.1"
                     /translation="MLLVTGACGYIGSHFVCHYLSQTDEDVVAVDNFSMGHRQALTPW
                     QGRIRFYDHDIADREALARLMKENQVSAVVHFAANAYVGESQVLPFKYLDNNVGGSTG
                     LLQAMAEAGVKRLVFSSTCATYGKPQYVPIDEKHPQKPINTYGSSKLMVEEMLRALAL
                     SKGLKFACLRYFNAAGASESGEIGESHEPETHLIPLALMAALGKIPQLKVYGDDYDTP
                     DGTCIRDYIHVLDLAAAHCQALDLLKRPDFVGDMEESDGLGLA"
BASE COUNT         1278 a         1745 c         1875 g         1640 t
ORIGIN      
        1 cggtccaatt cttgccccgc gcttcctgtc tgagggcttc caccagcggt tccaccgtcg
       61 gcagaccaag gtcggctttg atgaggcgct cttccagggc ttcgatgtat tcttcgtcta
      121 ccggcttgtg ggccgttgcc acgctggtag cggctgcggc tttgatgggc tcgggctgga
      181 cggctgccgc agtctgactt tcggctcctg ccgctgcggt ttcagcgccc tgggccagtt
      241 cttccgggtc ttgtacttgt tcttttgtct tggccagaac cgacttgagc cgggtcaggg
      301 tagtgcccca gaaacccttc ttcttatcga tgttatcctc gctcatttga acctcagttt
      361 tgagagatgc tttcagcttt ttctttggct agtttccttg cagcagctct tcttaccgcc
      421 tctgcttctt ccgaaaggtc gcccaggatg ccgtttatga atttggctgc ccgtgcatcg
      481 gcgaaacgat gggagagctc tacggcttcc gaaatagcta cgtttatggg aatgtcgggc
      541 atgtaaaagg tttcggcgca tgccaggcgc aggatatctc tgtcgatgga gaccatgcgc
      601 tccatgcgcc acttagactt ggagcgtttg atgaactcgt cgatggtttc tttatgttcc
      661 aggtagaccg aaataagtct taccacgaag tcgcgcactt cttctttgtg cggtttattg
      721 atggtggctt ccacgatggc accgcacttt ttacagttgg tgcgggtggt ttcggtgccg
      781 cgagccagag ccatttccgg aatgtccagg gcttccgaaa ccagagctat ggctgtttcg
      841 atggtttgca gtttttccct gaactgacta cttgttaggg ggactgctct caagtcttcc
      901 acgaagtgtt tgttgtcttc gtgctcggtt tcaatttgag tcaaatggtc ctgggcgcca
      961 atcaccagag cctgggcatc ggtgagattc tgtctggcat agtcgaccag catgtggatg
     1021 gctctgtcgg tgagctccat gatctgagtc tctgccagct tgtccaagtt cttgggcagt
     1081 tgcggcatca ctatcactgc cagttctcgc gctatgcgtc ttgcgctcat ttagatggct
     1141 tcctcgattt aactgtttaa agggttcgta gttgccttca aagccggcat ctctggctga
     1201 agtgactatg ttatcatggg aatttcagag gagtttggct tgcgcatcac tggtggagag
     1261 cttagaggta ggtctgtcac ttgcccccgg gggcttgctg tgagaccgac ttcgtcaaag
     1321 gtgaggcagg ctttttttaa cattttaggt gacagattga gacatgcccg tttcctcgat
     1381 ctgttctccg gtagcggttt aatgggtttt gaggcattga gtcggggggc cactgtcacc
     1441 tttgtagagc gtcacaaccc ttcccgggcg gcaattaagg cgacggccga gcaatttaag
     1501 ttgagtgagg agcgttaccg ggtcaggggc ggcgatgttc tcaaggtctt gccggaactg
     1561 gccggggagg aattcgacat tgtttttgcc gatccgccct ataaattgtg cctggggcct
     1621 gctgtggccg agcttcttgt tcgccacagg ctgctcagtg ccggtgcgct ttatgtagtg
     1681 gaacacatga gtgatgatcc ggtgactctt acagatggta gcctagagag agttgacttc
     1741 aggcagtatg gcgggacagc gatcagcatg ttccaacagg tatgtaccaa atgaggaccg
     1801 cttcgtgagt cagggcgaat taaccggcaa agaaactgaa aaacgagaca ggcgcgaagt
     1861 tggagtagac accggtcatg ggctgtcagg tgtttcctac acggcaagcg gcatcaatga
     1921 cgccctgtcc atggccatcg ggcatgtgcg cctgggcgat cttttgaagg cttcgggcat
     1981 tatcagctat gagttcctca aggaagccct ctctagtttt gaggagaagg ggctgcccct
     2041 gggaaaggtg cttactctct cgggctatct cactgccagt gagctggcgg tggctctgga
     2101 aattcaaacc ttgatcaaca accgacaact accctttgat gtgggggtga gggtgctggc
     2161 tatcagccat gatgagaagg ttccgctcag cgaggcgttt tccagggcca gtgtggtgca
     2221 gccggaggac ttactgagca acaagctcgg tcaacttctc agccagtctc gtctgataag
     2281 taacgaagac cttgaccagg ccctggctgt cagtcagcgc accggtctgc ctctgggaca
     2341 catcttttgc taccgcaatt tactctccca gcagttactg gacacggccc ttctgggcca
     2401 gcaattggtc agacgtggct ccctttcgcg agagaattgc ctggaggctc tatcggcggc
     2461 ccgagagagg gaagtggcac tgctttcctt gcccgtcaat gcagcctaca agccttccat
     2521 gcggcgcggt acatcgagac tgggggaact ctttttctcc gccggtatcc tggacgatat
     2581 gtccctcatt tctgccctgc agctctctct ctcccggggt attccctgtg gcgaggcaat
     2641 ttccgaagtg cagggcacca gtaccgctgt tattgaacag gctgtttgca tgcaggagat
     2701 gctcgacaac gagaccctta ctctagagca ggtcaaggat gtctttatcg gtatttttca
     2761 tcaggagcgc agcttcgaac atgctctggc ggaagccagc gtagcaggct gtagcggcaa
     2821 tccggccaga cttatggtac agcttttgaa agacgctcag attctggttt tacctgaaga
     2881 tagctcaacc actcctgcgg cactggcacc ggtgctgacc gaactgacta cctgccttga
     2941 tgtgaactat aaccagggcg gagtagtggg acgcctattg atttccgccg gtctggtcag
     3001 tgcttcggcg gcctgggcgg ctttgcgtct gacccattta ctgaccctgg aagtggtgcc
     3061 ctatgagcgg gccgtcgctg ccctggaggc ggcaaaccta caggggtcgg ttgatgaagt
     3121 gttaattggc ttcggcgcct tgaatcgcac cagactgagg tggcgctaga aaagtcagtt
     3181 ggatgagctg ggcgctgccg actctaccga ctctaccgtc tccgcttgcg gaatttcctg
     3241 tgcctgagca cagtcgcaag cttcatctct ggcttctact ctggcttcct ctctgacctc
     3301 cacctggggg gcgataggca tcttgcctgt ggtggtggcc gggtatttca gtctttcgtg
     3361 atggagggtg cgccagactc tggcaaagcc ggcttttact ttatgcagtt cttcaaggct
     3421 taggcctgat tcgctgaact gcccgtcttc ccagcggttc aaaaagacct tctccagcgc
     3481 ctgctctact tcttcctggc tggggtcgcg catagaatga gtgacggctt ccgaaacatc
     3541 agccagcatg acgatggctg tttctttggt attaggcttg ggaccaggat aacgatagaa
     3601 gttggcatcc accttgtcgg caccatctct ggtgcaggcc ttgtgataga aataggccat
     3661 cagcgtggtg ccctgatgca tggggatgaa gtcttgaatg gccttgggga gggcatattt
     3721 ctgggccagg gcaatgccgt ccgtaacatg ggccagtact cttgcccggc tgtcttccgg
     3781 ggacatgctg tcgtggggat tggtggcacc aagctggttc tcgataaaat acttggggcg
     3841 caccattttg ccgatatcgt gataaagggc accggcacgc accatgttgg catcggcata
     3901 aattgccttg gctccggctt ccgccatatt ggccacggcc aggctgtgtt gataggtgcc
     3961 gggagcgttt tcttccagcc gtctcaagag gggctgattg gcgtcggtca gctcggccag
     4021 acggaagggc gttatcagtc cgtagagatt ctccaggaag ggcagactgc ccaccgccac
     4081 tatggatgag atgataccgc cggcgaagtc cagaagtagg cggcgtccaa agatgcccag
     4141 attaccgccg tagctatgat ccaccagggt gctggccagg tcgccggcca ccagaccgaa
     4201 ggctaccacc agaccggttc tgaagacggc ggagcgaccg ccattggctc ccgtcccact
     4261 ctggggaaag ctcatgattg cccccaggga agcgagagta tgggcagcca ggtaatgcag
     4321 gtcaaccaac ctgtccacgc cgatgagcag caccatgggc aggctcaaag caatggccgt
     4381 gcgccggcca aaaaagatag taaggatgag ggctgccgct gggatgggca cgaactgcgg
     4441 attagtctta cccaccaggg aggccacgga agccacaatc acctggacgg tgaacatgag
     4501 accgatggaa ctgggagaga acaaatgctt gggctcgtag gtgtaaagga agagacccac
     4561 cagggtgagg gcggctatca aagagatggc catgctgaga atgaagggcc agcggttgac
     4621 gtgggtgatg cccaattcct gcaaaatgta gagtttttct ctggtgatca tggctccctg
     4681 gggcacgatc acttcccctt ccttaatggc tttcaggacg ggctctactc cttgcaccac
     4741 cacctgggcc ttggtcttgg tgccttccgt atcgatcacc aggttggctt ccaggttttc
     4801 attgatgacc agggccgccg tctgtttctt tccagcctcc aggctgtcgg gcaaaaattc
     4861 agtgaccacg tcctgccaga cacctttgtc taccaccggg tagcggtgga atttggccat
     4921 caggcgtctg gccgattcca gggtggcggc cttgaaggcc ggcatttccg cagggtccac
     4981 cgtcagagcc agttccaact ccagatcgcc gctcaaccct gctgttctgg cccggatata
     5041 ggctttgcgt tgggtttgaa tctccggtag atttgcctga gcctgaccca ccagcttgtc
     5101 atcgggtaat gtaagcaggc tcatttgcag tttcttgtcc atggaggatg gcgccggcac
     5161 tgcttccggc tgcctgttct tcatcttgcc ccgggctttg tttggttttt gagcgttgct
     5221 tgcctcgtga tagggcttaa tgcccatggc ttgcaaactc tcggctctgg cgaagacggt
     5281 ttcgatgcgc ttgagcgact cttgatcgcg gcttctgtct acctggaaga ccggaataat
     5341 actctgcctg gctttctcaa ttgctttttc agtttcagcc cggtctacta ccgatgcagc
     5401 ctgtggcgca aaaaggtccc ggtcggcaat gtccctttct tgcaagccgg ggctgaagat
     5461 gtgaatggtg gaaaaagaaa ggaagaggca gataaatcct gaaagtgcgg cgcccaccca
     5521 acccagcagg gcttgtggtg acggaaagtc ttctgccttt ttgaggctat cgtccacccc
     5581 ggtcgacaaa ttggctcctc agtacggtag cattgaaaat ctaccagttt cttgcacgct
     5641 tttcttgcgt cgacattcca ttttcagaga tgcttgcact ttatgccttc atattactct
     5701 ttttcggctg ctaaaattct taccgctggc gctcgggcgc tccttggagg ctgttaatgt
     5761 tactggtaac cggggcttgc ggatacatcg gcagccattt tgtctgccat tatctctccc
     5821 aaaccgacga agacgtcgtc gccgtcgata atttcagcat gggacacagg caggccctca
     5881 ccccctggca ggggcgcatt cgcttctatg accatgatat agccgatcgg gaggcgcttg
     5941 cccgcctcat gaaagagaac caggtcagtg ccgtggtgca ctttgccgcc aatgcctatg
     6001 tgggagagtc gcaggttctg cccttcaagt acctggacaa caatgtgggc gggtccaccg
     6061 ggctcctgca ggccatggct gaagcaggcg ttaagcgcct ggtcttttct tccacctgcg
     6121 ctacttatgg caaaccgcag tacgtaccca ttgatgaaaa gcatccgcag aagcccataa
     6181 acacctatgg ctcaagcaag ctgatggtgg aagaaatgct gcgagctctc gctctttcaa
     6241 aagggctcaa gttcgcctgt ctgcgctatt tcaatgccgc cggcgcttcc gaaagcggtg
     6301 agataggcga gagccatgag ccggaaaccc atttgatacc gctagccctc atggctgccc
     6361 tgggcaaaat tccgcaactg aaggtctatg gcgatgatta cgatacccca gacggcacct
     6421 gtattcgcga ttacatccat gtcttagatc tggctgccgc ccactgccag gccctcgatt
     6481 tactcaaacg ccctgacttc gtgggcgaca tggaagagtc tgatggactg ggccttgc
//
LOCUS       DAOQNZ010000024         6387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4718_length_6387_cov_3.866393, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000024 DAOQNZ010000000
VERSION     DAOQNZ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4718_length_6387_cov_3.866393"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1377)
                     /locus_tag="PKW73_00795"
     CDS             complement(<1..>1377)
                     /locus_tag="PKW73_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71845.1"
                     /translation="VLAGIYAVYVNNKALVHYNLKRAASSFGVPDYLSESIDRKSNLE
                     TIKETSRILEHYPNDVATRVSRAKAYKDLQRWKEALGDYDKLAEQEPSNSKWFEERGN
                     VNEYLCRYDAAIHDYSRAIELAGAETRLLITRAAAYRRAGKREKALEELKSLKTDEKL
                     DYSALSELCDLCCDLGEYERAHSALTRMIALDDSYYSNSGRRTRAGLYLKQGRLAEAA
                     EDYEVLCAAADKDVNCDDLFDLANVYDALGNDKNCQSARERAVRTLEGRLKEQGQALD
                     KGTESDSYYSFFDAETLLTFLEENKRDSEYETYLPLVMKLSKKNISDDGQDVDRLVGE
                     SGSISDLVEKLPEAEAKELATFFLGKIKNADTNGYDKEIRTIRAYAEGNLAPLRDGKV
                     GLANRYCYYEDEKDSGKEITPNEKQCHILHDKADQALDQEDLSKAMKLATDANSLDPE
                     QYCAVDTLL"
     assembly_gap    1378..1477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1478..2772)
                     /gene="preA"
                     /locus_tag="PKW73_00800"
     CDS             complement(<1478..2772)
                     /gene="preA"
                     /locus_tag="PKW73_00800"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HNA71846.1"
                     /translation="MADLSINFAGIKSPNPFWLASAPPSNSAYQVQKAFEQGWGGAVW
                     KTIGAPVLNVCNRYGTIDYKGSKIIGLNNVELISDRPIEINLKEMREVKRNFPNHAVI
                     ASVMVESNKEAWQEIIKRTEDTGIDGFELNFGCPHGMSERGMGSAMGQVPEYTCMVTE
                     WVMDVATKPVIVKLTPNVTDIVHPARAAVKGRASAISLINTVNSIMGVNLDTFEISPN
                     VGGKGGHGGYAGPAVKPIALHLLSAVGSDPEVAKAGLPISGMGGIETWRDAVEFMLLG
                     ATSVQVCTAVMHWGFRIVEDMIEGMSNWMDEKGFATCDQFIGKSLPRISSFGDFDLGF
                     QSVARINHDKCIQCNLCYIACNDAAHQCIDLKDLKLTSEVKERAWPVVREEDCVGCDL
                     CSRVCPVDDCITMVEIETGRPHITWNELVKTKPEVLDWE"
     gene            complement(2797..4179)
                     /locus_tag="PKW73_00805"
     CDS             complement(2797..4179)
                     /locus_tag="PKW73_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA71847.1"
                     /translation="MAEHYKPHKYKAWEMTLEERFQEVYPPFSEREATLEANRCLYCH
                     DAPCMVACPTHIDIPTFIRKIATGNVKGSARTILESNLMGATCARVCPVDVLCEGDCV
                     LGADHKPIAIGRLQRYATDYASERGIKFFQKGQPNGKKVAVVGSGPAGLSCAGELAKQ
                     GFDVTVFEKNEMAGGLSTYGIVVFREPVEVSLAEVKMVQDLGVTVKCGVTVGKDIKFD
                     DLVKDFDAVFIGIGLGNVPMMEIPGEDIGGVLDGLDFIAETKVKDLNKITYGKRICVI
                     GAGNTAIDCATIARRLGAERVTMIYRRSEAEMPAYHFEYEFALKEGVSFMFLTQPVEV
                     MAKDGKVDGLKCVRMDLGAPDASGRRSPYPVDGSEFVVPCDMVIKAIGQVKPAAFLSM
                     LEPYGVESVKGYIKVDEHNRTRHAKIFAGGDCVRSHGEASTVMAVQDGKIAARAITEA
                     LVATPACAKK"
     gene            complement(4260..5147)
                     /locus_tag="PKW73_00810"
     CDS             complement(4260..5147)
                     /locus_tag="PKW73_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNA71848.1"
                     /translation="MSNSVRCGLIQTKNEVISPAGGTDKKLLEEIKEKMLNKHLEYTK
                     QAAEKGVKILCYQEIFNGPYFCAEQQTCWYETAEEIPNGPTIKLMQETAKKYGMVLIV
                     PIYEKEQTGVYYNSAAVIDADGKYLGKYRKNHIPHVAPGFWEKFYFRPGNLGYPVFQT
                     QFATIGVYICYDRHFPEGARALGLAGAEIVFNPSATVAGLSEYLWKLEQPAHAAANGY
                     FVGAINRVGTEAPWNIGEFYGSSYFCNPRGQIIAQASRDQEELLTCDLNLDDIKEVRH
                     TWQFFRDRRPETYDQLVKL"
     gene            complement(5167..>6387)
                     /locus_tag="PKW73_00815"
     CDS             complement(5167..>6387)
                     /locus_tag="PKW73_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNA71849.1"
                     /translation="HFVKGKGQYLYDETGREYLDMFAGIVTVSVGHCHPKVTQRTVEQ
                     VQTLQHTSTIFMTQPMVELAEKLAEITPGDLSKTFVTNSGTEANESAVRFARLATGRH
                     EIIALEHSYHGESHLASTITGNHNWRPDTMPAAGVAYAANAYCYRCPFKKTPENCSLE
                     CAKDVERVIQTQTSGKPAVMIAEPIQGVGGAITPPDDYFKEVKTILEKYGALFIADEV
                     QTGVGRTGKHWFGITNWGVTPDMITMAKGLANGLPIGAVVASPKVVDGLVKQQINTFG
                     GNPVSTATACAVLETIEEEKLMENARVVGDYLMDGLRDLQKQFPDMIGDVRGKGLMVG
                     MELADKNKTPLTAETARIVEMAKDEGVVIGKGGLWGSTIRIKPPLTITKDNVDTMLKV
                     VRKCIEATVKVGAK"
BASE COUNT         1349 a         1715 c         1681 g         1542 t
ORIGIN      
        1 cagcaaagta tccacggcgc aatattgctc tgggtcgaga gaatttgcat ccgtagccag
       61 cttcattgcc ttagaaaggt cttcctggtc gagggcctgg tcggctttat catgcaaaat
      121 atggcattgc ttctcgttcg gcgtaatctc cttaccggaa tccttttcgt cctcgtaata
      181 acaatagcga ttagcaagac ccactttgcc atcccgcaag ggagcaagat taccttcggc
      241 ataggccctg atggtgcgaa tctccttgtc atagccattt gtgtcggcat tcttgatctt
      301 accgaggaag aaagtcgcta gttccttcgc ttcggcttcg ggcaatttct ccactagatc
      361 tgaaatggag ccgctttcac ctaccaatcg gtcaacatct tgcccatcat cgctaatgtt
      421 ctttttggaa agcttcatca ccaggggaag ataggtctcg tattcactat cgcgcttgtt
      481 ctcctccagg aaagtaagca aggtttcggc gtcgaaaaag ctgtaataac tgtcgctctc
      541 ggtgcctttg tccagagcct gcccctgctc cttcaagcgc ccctccagtg tacgcacggc
      601 tctttcacga gcggactgac agttcttatc attaccgaga gcatcataga cattggccag
      661 gtcgaacaag tcatcgcagt tcacatcctt gtcggcggca gcgcagagga cttcatagtc
      721 ttccgccgct tccgccaacc gtccctgctt caaataaagt ccggccctcg ttcttctccc
      781 agaattagaa taataagaat catcgagagc aatcatcctg gtgagagcac tgtgggcacg
      841 ctcatattca ccgagatcgc aacaaagatc gcaaagctca ctcagtgccg aataatcaag
      901 tttctcgtca gtctttagac tcttgagttc ttctaaggcc ttttctctct tgccggctcg
      961 cctgtaggcc gccgcacggg taatcaaaag cctggtctcg gcgccggcta gttctatagc
     1021 ccggctatag tcgtgaatag cggcatcgta ccggcagaga tactcgttga cattgcctct
     1081 ttcttcaaac cacttactat tggaaggctc ttgctcggcc aacttatcgt aatcgccaag
     1141 ggcttccttc catcgctgca gatctttgta tgctttagca cgggagaccc ttgtggccac
     1201 gtcgtttggg tagtgctcca ggatgcggct ggtttccttg atggtttcca gattcgactt
     1261 gcggtcgata gattcgctca aatagtcggg cacaccaaag ctggatgcgg cacgcttcaa
     1321 gttgtagtgc accagcgcct tattgttcac atagacggcg tagatgcccg ctagaacnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt tcccagtcta aaacttccgg
     1501 cttggttttc acaagctcgt tccaggttat atgggggcga ccggtttcta tttccaccat
     1561 ggtgatgcag tcatccacag ggcagactct tgagcaaaga tcgcaaccaa cgcaatcttc
     1621 ttcacgcact accggccagg cccgctcctt cacttccgat gtaagcttca aatctttcag
     1681 gtctatacac tggtgtgcgg catcattgca ggcgatgtag cagagattgc actgaatgca
     1741 tttatcgtga ttgatgcgag ccaccgactg gaaaccaaga tcgaaatcac cgaaactgga
     1801 aatgcgcggc aaggacttac cgataaactg atcgcaagtg gcaaagcctt tctcgtccat
     1861 ccagttggac atgccttcaa tcatgtcttc cacaatgcgg aaaccccagt gcatgacggc
     1921 cgtacagacc tgcacgctgg tggcaccgag caacatgaac tccactgcat cacgccaggt
     1981 ttcaatgccg cccataccgg aaatgggcag tccggctttg gccacttccg gatcggagcc
     2041 aaccgccgag agcaaatgca gggcaatcgg tttgacggcc ggtccggcat aaccaccgtg
     2101 accacccttg ccgccgacat tagggctaat ttcgaaggta tccagattta cgcccatgat
     2161 actgttgacg gtattgatga gagaaattgc cgaagcgcgc cctttgacgg cggcccgggc
     2221 cggatgcacg atgtcggtca cgttgggtgt gagcttgacg atgaccggct tggtggccac
     2281 gtccatgacc cattccgtca ccatacaggt atactccggc acctgcccca tggcagagcc
     2341 catgcctctt tcggacatac catgaggaca gccgaagttc aattcgaagc cgtcgatacc
     2401 ggtgtcctcg gtacgtttga taatctcctg ccaggcttct ttattagact caaccatgac
     2461 cgaggcaatg acggcatgat tggggaagtt gcgtttgacc tcccgcattt cctttaagtt
     2521 gatctcgatg gggcgatcgg aaatgagttc gacgttgttg agtccgatta ttttgctgcc
     2581 cttgtaatcg attgtgccgt agcgattgca aacgttcaag accggtgcac caatggtctt
     2641 ccaaaccgct ccgccccagc cctgctcaaa tgctttctgc acctgatagg cagaattcga
     2701 gggcggcgcg gaggctaacc agaaggggtt gggtgacttg ataccggcaa aattgatgga
     2761 aagatcggcc atttctttgg tcctttttta aaattgttac ttcttggcgc aggcgggcgt
     2821 tgccactaaa gcttcggtaa tggcccgggc ggcgattttg ccgtcctgca cagccataac
     2881 tgtagaagct tcgccgtgac ttctgacgca gtcaccaccg gcgaaaattt tggcgtgcct
     2941 ggtgcggtta tgctcatcta ccttgatata gccttttacc gattcgacac cgtagggctc
     3001 cagcatagac agaaatgcag ccggcttcac ctgcccaatt gctttgatga ccatgtcgca
     3061 gggcaccacg aattcggaac cgtccaccgg atatgggctg cgacgaccgg aagcatcagg
     3121 cgcgccgagg tccatgcgca cgcacttgag accgtctacc ttgccgtcct ttgccatcac
     3181 ttccaccggt tgggtgagga acatgaagct gaccccttct ttcagggcga attcatattc
     3241 gaagtgatag gccggcattt ccgcttccga gcggcggtaa atcatggtca cccgctcagc
     3301 gcccaggcgc ctggcgatgg tggcacagtc gatggcggta ttaccggcac caataacgca
     3361 aatgcgcttt ccataggtaa tcttattgag atctttgacc ttggtctcgg caatgaaatc
     3421 cagaccgtcc agaacgccgc caatgtcttc gcccggtatt tccatcatgg gcacattacc
     3481 gaggccgatg ccgatgaaaa cggcgtcgaa gtctttgacc aggtcgtcga atttgatgtc
     3541 tttaccgacg gtgacaccgc atttgacggt gacgcccagg tcttgcacca tcttcacctc
     3601 ggccagactg acctccaccg gttcccggaa gacaacaatg ccgtaagtgg aaagaccgcc
     3661 ggccatctcg tttttctcga agaccgtcac atcaaaacct tgcttggcca actcgccggc
     3721 gcatgacagt ccagccgggc cggagcctac aacggctacc ttcttgccgt tcggctgtcc
     3781 tttctggaag aacttgatac cacgctcact ggcatagtcg gtggcatagc gttgcaagcg
     3841 gccgatggcg atgggcttgt ggtcggcgcc caggacgcag tcaccctcgc agagtacgtc
     3901 caccgggcag actcgcgcac aagtggcacc catcaaattg gattcgagga tggtacgggc
     3961 agaacctttg acattgcctg tggcgatttt gcgaataaag gtaggaatgt cgatgtgggt
     4021 gggacaggcc accatgcaag gtgcatcatg gcagtacagg cagcggttcg cctcaagggt
     4081 ggcttcccgt tcggaaaagg gcggatagac ttcctggaag cgctcttcca gggtcatttc
     4141 ccaggccttg tacttgtgcg gtttgtaatg ctctgccatt tagaacctcg ctaagtttca
     4201 aaaaaaagaa atagccgccc caccattcaa gggaactcgg gggcggcgtg tctatctatt
     4261 tagagcttga ccaattgatc ataggtctca ggacgtctgt cgcggaagaa ctgccaggtg
     4321 tggcgcactt ccttgatgtc gtcgagattg agatcgcagg tgagcaattc ttcttgatcg
     4381 cggctggctt gtgcgattat ctgaccgcgc ggattgcaga agtagctcga gccgtagaac
     4441 tcgccgatat tccagggagc ctcggtaccg acccggttga tggcacccac gaagtagccg
     4501 ttggcggctg cgtgagccgg ttgttcaagc ttccagagat attcggaaag tccggctacg
     4561 gtagcggaag ggttgaagac aatctccgcg ccggcaagac cgagggcacg agcgccttcc
     4621 gggaagtgac ggtcgtagca gatgtaaacg ccgatggtgg cgaactgcgt ctggaagacc
     4681 ggatagccca ggttgcccgg gcggaagtag aatttttccc agaagccggg ggctacatga
     4741 ggaatgtgat tctttctgta tttgcccagg tatttaccgt cggcgtcgat gacggcggcg
     4801 gaattatagt agacgccggt ctgctctttt tcataaatag gtacgattaa aaccatgccg
     4861 tactttttgg cggtttcttg catgagcttg atggtggggc cgttgggaat ttcttccgcg
     4921 gtctcgtacc agcaggtttg ttgctcggcg cagaaatagg gaccgttgaa gatctcctga
     4981 tagcagagaa tcttgacgcc tttctcggcg gcttgcttgg tgtactcgag atgcttgttg
     5041 agcatcttct ctttgatctc ttccaacaac ttcttgtcgg taccgcctgc cggcgatatg
     5101 acttcattct tggtttgaat caagccacaa cgcactgaat tagacattat ttgctctcct
     5161 cttgctttac ttggcgccga ccttgacggt ggcctcgata catttgcgaa ctaccttaag
     5221 catggtatcc acgttatctt tagtgatagt gagtggcggc ttgatacgga tggtgctgcc
     5281 ccagagtccg cctttaccaa tgaccacacc ttcgtcctta gccatttcta cgattctggc
     5341 ggtctcggca gtgagggggg tcttgttctt gtcggccagt tccataccga ccatcaagcc
     5401 tttgcctcgc acatcgccaa tcatgtccgg gaactgcttc tgcagatctc tgagaccgtc
     5461 catgagatag tcaccgacca cgcgggcgtt ctccatgagc ttttcttctt cgatggtctc
     5521 cagcacggca caggcagtgg cagtggaaac agggttgccg ccgaaggtgt tgatttgctg
     5581 cttgaccagg ccgtccacaa ccttgggaga cgccaccacg gcaccgatag gcaatccgtt
     5641 agcgagccct ttggccatgg tgatcatgtc cggtgtgacg ccccagttgg tgataccgaa
     5701 ccagtgcttt ccggtacgac cgacaccggt ctgtacttcg tcggcaataa agagagcgcc
     5761 gtacttttca aggatggtct tcacttcctt gaagtaatcg tcgggagggg taatggcccc
     5821 gcccacaccc tgaatgggtt cggcaatcat gaccgccggc ttgccgctgg tctgggtttg
     5881 aatgacccgc tccacatctt tggcacattc aagggagcag ttctcgggag tcttcttgaa
     5941 cggacaacgg tagcagtaag cgttggcggc ataagcgaca ccggcagcag gcatggtatc
     6001 aggacgccag ttgtggttgc cggtgatggt ggaagccagg tgcgattcgc cgtgataaga
     6061 atgttcaagg gcgatgatct cgtgacgacc ggtggccaga cgggcaaagc gcacagcgga
     6121 ttcgttggct tcggtaccgg agttggtgac aaaggtcttg ctcaagtcac cgggagtgat
     6181 ctcagccagt ttctccgcca gctcaaccat gggctgggtc atgaagattg tggaagtgtg
     6241 ctgcaaggtc tgcacttgct cgacggtgcg ctgggtgacc ttggggtggc aatgaccgac
     6301 ggaaaccgta acgatgcctg cgaacatatc caggtattcc cgcccggtct cgtcatagag
     6361 gtactgcccc ttgcctttca cgaagtg
//
LOCUS       DAOQNZ010000025         6305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4803_length_6305_cov_4.162720, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000025 DAOQNZ010000000
VERSION     DAOQNZ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4803_length_6305_cov_4.162720"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1154)
                     /locus_tag="PKW73_00820"
     CDS             complement(237..1154)
                     /locus_tag="PKW73_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71850.1"
                     /translation="MSVRNSDGSSSSGEGRLQAQADALRFGAGEPGKSGAGEPGKSGA
                     GDLGKNGSGDLGKTEVARVGDQKQPPLPDGDVTTFKSQNQALERIGGALFRDSAAPHL
                     EHLQGLDPVAGGAITRQGMRKFLKEADLNGDGKSVNEKYPQDFVQSVAMLERGFDSPS
                     LKAFKDENGNISKESYTKGIFNRQDRLAAMGEKANAVKPGGGEEKALDGKLKEASVIK
                     AGEGPFAAAQRILESLGGEVSRAELRQLTRALKEQYIADHGGKKNSINSLKVGHAMLD
                     NPRTLEGVLGRLKNDSLKERLTKGSRATA"
     gene            complement(1267..1683)
                     /locus_tag="PKW73_00825"
     CDS             complement(1267..1683)
                     /locus_tag="PKW73_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71851.1"
                     /translation="MWFERYYFFVSIASFAIQIVLWASRVLLGFKSFFALSFMPGLMV
                     FQWLISLTAIMVFIVSGKLKLLPAPAVYLLFSALIFIWEQASAFMAAARFENSKGFGA
                     ETPYLEIVMLLVSLAVHLLFLVPAARKQGAGASAGD"
     gene            complement(1737..2057)
                     /locus_tag="PKW73_00830"
     CDS             complement(1737..2057)
                     /locus_tag="PKW73_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNA71852.1"
                     /translation="MNLKPFAYYMVLLPFLVWSGYYAGCEVARGEINVQKLENVSVMV
                     KLVKAKTDVPPQVVLGSQHLEEVEVFANRQVGGELQQLKQALGKKSHYGLVKGQVLLE
                     QELE"
     gene            complement(2054..2473)
                     /locus_tag="PKW73_00835"
     CDS             complement(2054..2473)
                     /locus_tag="PKW73_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71853.1"
                     /translation="MSSSNSSKQLLVILGSLAIFLGSIGLGYFWGFSSTWAHFKKQPL
                     IGKEPITLQLVKVVRDIPASGKVEFENVEEVRVKMPQVYADSLGFASECMGRQVKWTL
                     RKGGYVSRHDLVPVGKADANLRSAQDDESQPKAGGVR"
     gene            2822..3952
                     /locus_tag="PKW73_00840"
     CDS             2822..3952
                     /locus_tag="PKW73_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71854.1"
                     /translation="MQKRRRPDGNTAEFPVALMLLLLVGVFPVLDLLFCGIAACCTYF
                     AANQICNRAASQRTYAEALNAAADEARVFNNSGFAAFARMAPVSGYSGSGMDLFVAQT
                     STDVGNAIKKWGPNTPVTDPIDKNAFVYEYATKVEYDVSPLVKMDGLPFISSIPGLGT
                     NYKLSMTAHRAAEYPLGLSGPGSALALKGGSAPKNLRSPGIQTMGFVADPESEGWRTP
                     NIYDMIKQSGRKIVTQDVLIIDSRSFNWTPTSLNVPTGAQIWVDYKADGAWNVKPSDS
                     PVDFDANGQTICPDSPFPCGMMIGKVGLNGSPFALGVDQWNFPLPGSGTLYMGCKEGS
                     NGITPSSIGLSEPGQPQPPLAGTYGDRADNRGTMTVRVIITQ"
     gene            3949..5574
                     /locus_tag="PKW73_00845"
     CDS             3949..5574
                     /locus_tag="PKW73_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HNA71855.1"
                     /translation="MRKQSTATRRRSGNILIFAIAALSVLVAIGAVYGVNLTRFMGAH
                     QEQVSAVESAALVAARDLEKIVVDDPFVGLVSLATFPPTGKGTLAQDDFYMPVQGINS
                     LLATVRLETVLADKFDDETLRRLAKRDYDLATQARVRLVDVLKSAIVKDGFGYDYDGN
                     KVEPLKDAEAAYLAASQRMAAGKTTLVKDSISLELGLIDGLVTNTRVPNPSTFAEMED
                     TAQQSGFYRAYVNVPFKNYNFVFAGISDGTNLVDGKLFKLTEPSLPYVIPAIVRCHAD
                     QLYEDPNPGHASEAHTVHVSACAQPGSLGDTKPSAGVLSLTFPNGAIPEITTLHALLM
                     EPQIVTSPSDVTQAPMTGDSPPETPVQIKVPIVNDDHAPVGQLMRIAFFDWVRRAGPN
                     MNVQSLLDALDTPLSDSAQPHRELFMHRADGTITHTTRPQDPAVSLPISDRQFYAASG
                     LALHSNDTFSYDFYIRDYCFQPGRTNGGKHGGEPLGDAPPTPPPPVPGDPPLALDEYP
                     ALISAFPTGPGGGANRPTYNNPGVAVDFRLERR"
     gene            5584..5766
                     /locus_tag="PKW73_00850"
     CDS             5584..5766
                     /locus_tag="PKW73_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HNA71856.1"
                     /translation="MLDEHYFECPYCWENISMLLDLSGGAQSYIEDCEVCCNPIRISY
                     DVEDEQLIDFRAEQYG"
     gene            5945..>6305
                     /locus_tag="PKW73_00855"
     CDS             5945..>6305
                     /locus_tag="PKW73_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71857.1"
                     /translation="MQTLTEEDRQTIEAHTLLEEALKLGSTGDRQKELELLKQAEKLA
                     STPLLADRSEYGFIYGDLGRWSEAIKEFDSYFEKEGAYAPRNIMVVDYLIKHSKADVA
                     LRFSRTLSKLAPTAELLD"
BASE COUNT         1624 a         1898 c         1468 g         1315 t
ORIGIN      
        1 cgctagtcgc tcaaaaagaa tcgcgcccgg cggctctttc gaaccaccgg actcgaatct
       61 ctacctcccc acgaaaagca atcaaataga ccggctgtgt tgacttacat ttattgccgc
      121 ttctcttgcg ccagtggcac cgcgtctctt tgtgctacta atctattgcc gcttccgtga
      181 aaaaagcgtg agcaaattta agaccactaa aacttttttc gttccggaaa attactctag
      241 gctgttgccc ttgaaccctt ggtcagacgc tctttcaaag aatcattctt gagtcgaccc
      301 agaacgccct caagcgtccg gggattatcc agcatggcat gaccgacctt gagtgaatta
      361 atggaattct tcttgccgcc atgatcagcg atatattgct ctttcagggc gcgagtcagt
      421 tggcgcagct cagcccttga aacttctccg cccaggcttt cgagaatccg ctgagctgcg
      481 gcaaagggac cctcaccggc ttttatcacg gaagcttcct tcaacttgcc gtcgagggcc
      541 ttctcttcgc cgccgccggg cttgacggcg ttagctttct ctcccatggc ggcaaggcgg
      601 tcctgcctgt taaagattcc ttttgtataa gactctttcg aaatattgcc gttctcatct
      661 ttgaatgcct tcagcgatgg actatcgaag cctctttcaa gcatggctac agactgcacg
      721 aaatcttgcg gatacttctc attaacgctc ttgccgtcac cattgaggtc ggcttctttg
      781 agaaacttgc gcatgccttg cctggtaatg gccccgcccg ccaccggatc cagaccttgc
      841 aaatgttcaa gatgcggggc ggcactatcc cggaagagag cgccgccaat tcgctccagc
      901 gcttgattct ggctcttgaa agtagtcaca tccccatcag ggagaggggg ctgcttctgg
      961 tcaccgaccc tggctacttc tgttttgccg agatcgcctg atccattctt gccgagatcg
     1021 cctgcaccac tcttgccggg ctcgcctgca ccactcttgc cgggctcgcc tgcaccaaaa
     1081 cgcaaggcat cggcctgggc ctgcaatcgc ccttcgccgc tggagctgga gccgtcgcta
     1141 tttcgcacag acaagtctgt acgctcttca acacctgcaa aggattttgc cgacatattc
     1201 aatcaatctc aaactgagga aacgggaggt aaattgcagt ctaagcgccg cggcgtaaaa
     1261 aacttgttaa tccccggcag atgctcccgc cccctgcttc ctcgccgccg gcacgagaaa
     1321 cagcaggtgc acggctaaag aaaccagcag catcactatt tctaaatagg gagtttcggc
     1381 accgaatccc ttactattct cgaagcgagc agccgccata aaggccgagg cctgttccca
     1441 gataaaaatc aaggcactga ataacaaata aaccgccggg gccggcaaca acttcaactt
     1501 gccggagacg ataaaaacca ttattgccgt aagggaaatc agccactgaa aaaccatcaa
     1561 acccggcata aaagaaagag caaaaaaact tttgaaacca agcaaaaccc ggctcgccca
     1621 gagaacaatc tgaatggcga aagaggctat ggagacaaag aaatagtaac gctcaaacca
     1681 catcaattac ctttcaattt ctccagccgc tccggtttga cgacacaagc gaaactctat
     1741 tcgagctcct gctccagcaa aacctgtccc ttgaccagtc catagtgaga tttcttacca
     1801 agggcctgct tcaactgttg cagttcgccg cccacctgcc gattggcaaa tacttccact
     1861 tcctccagat gctgcgaccc tagcactacc tgcggtggca catccgtttt cgccttgacg
     1921 agcttaacca tgaccgaaac attttccagc ttctgtacat tgatttcgcc ccgagccact
     1981 tcacagccgg cataatagcc cgaccaaacg agaaacggca aaagtaccat gtaataagca
     2041 aagggcttca gattcacctt acccctccgg ctttcggttg gctctcatca tcctgagcgc
     2101 tcctcaaatt agcgtcggct ttccctacgg gtacgaggtc atgccggctg acatagccgc
     2161 ccttgcgcaa agtccactta acctgccgcc ccatgcattc agaagcaaaa ccgagagaat
     2221 ctgcataaac ttgaggcatc ttgacccgca cttcctccac attctcgaat tccactttgc
     2281 cggaggcggg aatatcgcgc acaactttta ccagttgcaa ggtaatgggc tctttgccaa
     2341 tcaaaggttg tttcttaaaa tgagcccagg tggacgaaaa accccaaaaa taaccaagcc
     2401 caatagaacc cagaaatata gccaggctgc cgaggattac cagaagttgc ttggaggaat
     2461 tggaactgga catacttcgc tcaatttaac taacctaagg atacctgcct gcagatggtt
     2521 tagacacaaa ataaacggca tgtaaaaaag caaccgccgc agtcagacca ccaaactttc
     2581 gctacagcca aaccaccatt actttgctgg tcgaaaagcc caacttgcaa tagtcgtgct
     2641 aaccgtaaac tggagctacg ctgccaccga tttccctcta cttcaagggc taagggtacc
     2701 atgctaagcg agcaaagata atgttttccg gagtttcaga cacgtatatc cgggtaggga
     2761 atatatacat caggcagacc gcttactctc gctcaggcat catcagccgc aggttcaaaa
     2821 tatgcaaaaa cgaagaagac cggatgggaa caccgccgag tttcccgtag cgctaatgct
     2881 gcttctcctg gtaggagtgt ttccggttct agacttactg ttttgcggta ttgccgcctg
     2941 ctgcacttat tttgccgcca accagatctg taatcgcgcc gcttcccagc gcacttatgc
     3001 agaagccctg aatgccgccg ccgacgaagc cagggtcttc aacaattccg gcttcgccgc
     3061 ctttgccagg atggcccccg tcagcggcta cagcggcagc ggcatggatc tctttgtagc
     3121 gcaaaccagc actgatgtgg gcaacgccat aaaaaaatgg ggaccaaaca cacccgtgac
     3181 cgaccccata gataagaacg cctttgtcta cgaatacgcc accaaggttg aatacgatgt
     3241 ctcgcctcta gtcaaaatgg acggactgcc tttcatatct tccattcccg gtctgggcac
     3301 taactacaag ctctccatga ccgcccacag agcagccgag tatcctctcg gattgagcgg
     3361 acccggcagc gccctggccc tcaaaggcgg cagcgctccc aaaaatctca gatcaccggg
     3421 catccagacc atgggttttg tggccgaccc cgaaagcgag ggctggagaa caccaaatat
     3481 ctacgacatg atcaaacaaa gcggtcgaaa aattgtcacc caggatgttc taataatcga
     3541 ctcccgcagc tttaactgga ctcccacctc ccttaacgta ccaaccggcg cccaaatctg
     3601 ggtcgactac aaggcagacg gcgcctggaa tgtgaagccc agtgattcgc cggtcgactt
     3661 cgacgcaaac ggtcaaacta tctgtccgga ttcacccttc ccctgcggaa tgatgatagg
     3721 caaggtaggc ttgaacggtt caccctttgc cctgggcgtt gatcagtgga acttccccct
     3781 acctggaagc ggcactcttt acatgggctg taaggagggc tcaaatggaa tcactccctc
     3841 cagcatcggc ttatcagagc cgggtcagcc gcagccgcct ctggcaggaa cctatggcga
     3901 ccgcgcagat aacagaggca ccatgactgt cagggtaatc atcacccaat gagaaaacag
     3961 tctacagcca ctcgcagaag aagcggcaac atactgatct ttgccattgc cgcgctgtca
     4021 gtactggttg ctatcggcgc tgtatacggc gtgaacttga cacgcttcat gggcgcccat
     4081 caagagcaag tttccgccgt ggaaagtgcg gcactggtgg cagcaagaga cctggaaaaa
     4141 atcgtggtgg atgacccctt tgtgggcctg gtctccctcg cgacttttcc acccaccggc
     4201 aaaggaaccc tggcacagga tgatttctac atgccggtgc agggcatcaa cagtttgctg
     4261 gccacagtta gactggaaac cgtgctggcg gacaaattcg acgacgagac cttgcgaaga
     4321 ctggccaaac gagactacga tctggccacc caagcaaggg taagattggt agacgttctt
     4381 aagagcgcaa tagtaaaaga cggcttcggc tatgactatg acggcaacaa agttgagccc
     4441 ctcaaagacg cagaagcagc ctatttagca gccagtcagc gaatggccgc cggcaagacc
     4501 accctggtca aagactccat aagtctggaa ctcggtctca tagacggtct ggtgacaaac
     4561 actcgcgtcc ccaatcccag cacctttgca gagatggaag ataccgctca acagagcggc
     4621 ttctaccgag cctacgtcaa tgtaccattc aagaattaca acttcgtctt cgcaggtatt
     4681 tccgacggca ccaacctggt agacggcaaa ctcttcaaac tgaccgaacc cagcctgccc
     4741 tatgtaattc cagcgatcgt ccgctgccat gccgatcaat tgtacgaaga tccaaatccc
     4801 ggtcacgcct cggaagcaca cacggtacac gtctcagcct gcgctcagcc aggaagcctg
     4861 ggcgatacca aaccctcagc cggggtgctc agtctcacct tccccaacgg cgccataccg
     4921 gagataacca cactacatgc actcttgatg gagccccaaa tagtcaccag ccccagcgat
     4981 gtcacccagg cacccatgac cggcgattcc ccgccggaaa caccggtaca aatcaaggtg
     5041 ccaatcgtca acgatgacca cgcccctgtc ggacaattga tgcgcatagc cttctttgac
     5101 tgggttagaa gggccgggcc gaatatgaat gttcagagcc tgcttgacgc cctcgacacc
     5161 cccttgagcg acagcgccca accccaccgt gagctcttca tgcacagagc cgacggcact
     5221 atcacccata ccaccagacc acaagatcca gccgtcagct tgccaatcag cgacagacaa
     5281 ttctacgccg catccggatt ggcactgcat agcaatgaca ccttcagtta cgacttttat
     5341 atccgcgatt actgcttcca acccggtcgc acaaacggcg gcaagcacgg cggcgagccc
     5401 ctgggagacg caccgcccac accaccacca ccggtaccag gcgacccacc cctggccctg
     5461 gatgaatatc cggctctcat cagcgccttt ccaaccggtc ccggcggcgg cgccaaccga
     5521 ccgacttaca ataaccccgg cgtagccgta gacttcagac tggagcgcag ataagcacag
     5581 tcaatgctag acgaacacta ctttgaatgc ccctactgct gggagaacat atcgatgctg
     5641 cttgacttga gcggcggcgc ccagtcttac attgaagact gtgaggtttg ttgcaatccc
     5701 atccgcatta gttatgatgt agaagacgaa caattaatcg acttccgcgc agagcaatac
     5761 ggttagcaaa aactcaatgc aaatccctaa agcggcactt cggtgcggta ccataacatt
     5821 atacgccctg gtggcaatgc tgctagtgac tggtcagcca ccgaatgccg ccgctcaggc
     5881 atgtcaacta ccgccaccat cggcactacc accgccaccc ttaccggatg gcaggcaagc
     5941 gcccgtgcaa accctcactg aagaagatcg ccaaactatc gaagcacaca ccttgctgga
     6001 ggaagctcta aaactcggca gcacaggcga tcgacaaaag gagctggagt tactcaaaca
     6061 ggcagagaag ctagcttcaa cgccgcttct ggcagaccgc tccgagtatg gctttatcta
     6121 cggcgatctg ggtcgctgga gcgaagccat aaaggaattt gattcctact ttgagaagga
     6181 aggtgcctat gcacccagaa acataatggt cgtcgattac ttgatcaaac atagcaaagc
     6241 agatgtagca ctgcgcttca gtagaactct gagcaaactt gcaccaaccg ccgagttact
     6301 ggact
//
LOCUS       DAOQNZ010000026         6305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4805_length_6305_cov_4.052480, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000026 DAOQNZ010000000
VERSION     DAOQNZ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4805_length_6305_cov_4.052480"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKW73_00860"
     CDS             <1..424
                     /locus_tag="PKW73_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71858.1"
                     /translation="KAAKAESDMAPESGQFPRRAGLANLFLAIKHINDASVAENVKQR
                     FSDYVTIVLVVMCILSLVMAVSVPVSPVLKLLPLLFTVGSVVFYIINRLGIMISLTAR
                     QALIVWQILVAGFWLGVTSAMLVMLACFYFVAQTTPGL"
     gene            567..2084
                     /locus_tag="PKW73_00865"
     CDS             567..2084
                     /locus_tag="PKW73_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA71859.1"
                     /translation="MGRLVQPGKESEDKNSQLVLGMVWEPVSFNPLRGIDSGSYCASS
                     LVYEGLVKFDENLDLVGGLAESFSVSQDGLTYRFRLRPNLRYSDGEKIEAADIKASLL
                     LGASSISPFRSDYKDMKEVVIEDERNLVVVLERPCQPLLSRMAELRILPTHILQSADH
                     GNKRLGRSPICTGAYQLKRWKAGQELVFERNPYYWGRPAETASIIWRVIPDKMALSAA
                     LARGEVDLAPVDGRLWQNFLSKSKEHLAMAEFNGGRTVYLGFNLQKAPWNNLKVRQAF
                     ASAINRGAMVDAFYSGHAVVPNTDVPLTSWAFEPNLPRADFAPEKVPALLQEAGFKKE
                     GKVWLKDGKVLALRILTIKDFEEMAQAVADYLRRVDIAAEVEVMEYSTLRRAFMQKGL
                     FDVILWSRSFGPDPECSMVWSSGGALNFCRLQDARVDGWLREARLAPNRQARKAIYRK
                     FQEYLATELPWLFLAQPRQIMAYREGMVGIQKGRQKDAGLPWDNIAANAAFWKHR"
     gene            complement(2268..2480)
                     /locus_tag="PKW73_00870"
     CDS             complement(2268..2480)
                     /locus_tag="PKW73_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71860.1"
                     /translation="MRSALLVLAALAAVSVPAANAQVCGDEGRYFHLRPPSYSISRHR
                     KQCIHRWAKALRWYREHNGDKPLCTK"
     gene            3145..>6305
                     /locus_tag="PKW73_00875"
     CDS             3145..>6305
                     /locus_tag="PKW73_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNA71861.1"
                     /translation="MSKAVRSDHVPNRVPPQPGECRDAVHWFVDSVGLYQGSPRGLIL
                     SDYSEDQEALIESAVINFKSRGVTSMVIAGDMLYKQADYLYQHAAAQTTFGRPLLSKL
                     ELDMLDSEVLIVTDLVAPETPQQLWYFYHHLLYPRALAQKPLVITTPLGLDEFFTYGA
                     ECDDLEYAGRRVTWEKAAWLMDGMLIDLHHFRMLRAENLPPMLKVEYFLYKAVVSRGL
                     NLLPQHVMGDYMVDFALMERGNKLAIECDLLASLDNPNSRSAEATRTLVLLSDGWKLL
                     RFTTVDLLANLMDCVDAVADVWVTGRKKSPSGRLVSGQSSSHLLELPVEDDVERQCIT
                     SGAGPVAITGGAGTGKSTCVVRRVAHLLAQGVNPERILVISHSTESVRSLRLDIESII
                     DKPTLQKLHFHSWNELGLKLLKENTSAIKRKPPLKVEGNPQRVIQRLLTKFKRDLDQL
                     TLELSEELEEFTIASLISLYKANLITPKHVKERAKTSVDELVAKVYQGYEEQLQKSNK
                     IDRDDMVTLAAQLLADNADIRARYQYQYEFVLVDEFQDANAASDLMARMLSLPQDNLF
                     IAGDEDQSIYESKGALPKILSEVSLRLPNARCYVLENNWRSHPEIVELAGRLLEGLSR
                     RRIRKDMVPGWEMSGERAVIGPQLAESEAAEAEWVAQEIQGLLSEGRNPQDIAILYRY
                     NRYASLVEEALFRKGIKCLTTNPDSTMVPDEVADVMAFLRLVMDPDGPKARESFERIC
                     QLRTKEIDPKLFNTIASFAEANNLSFLKAVEIYSEAVADQSCQDLEQLVRIIRTMNHE
                     NLPPAETIALLKRTQRLSEYYKAVKVPPGVNYEPMRKLSQLEEEARKFKTVSEFVRSQ
                     TSQKSADGQAEPLVYILSLHESKGKEFPVVFLTGMAEGLFPSETAGDPEEERRLCYLG
                     MSRAKDMLYVSYPGKFNDVVLAPSRFLLETMQDSTPKPVSAAGATVQPGAASAAGATM
                     QAQQEAALKAQQEAALRAQQEAAQRAQQEAALRAQQEAALKAQQEAALRAQQEAALRA
                     QQEAALRAQQEAALKAQQEAALRA"
BASE COUNT         1443 a         1569 c         1799 g         1494 t
ORIGIN      
        1 gaaagcagct aaagctgagt ctgatatggc tccggagtcg ggtcaatttc cccgacgggc
       61 cggtctggcc aacttgtttt tggcaatcaa gcacataaac gacgcatcgg tagctgaaaa
      121 cgtaaagcag cgcttttccg actacgtgac catcgtactg gtggttatgt gcattttgag
      181 tcttgtaatg gcggtttccg tgccggtttc gcccgtactc aagctcttgc ccttactttt
      241 cactgttggt tcggtggttt tttacatcat caaccgcctg ggcatcatga tttcgctcac
      301 cgccaggcag gctttgatcg tctggcaaat tctcgtagcc ggcttctggc tgggcgttac
      361 cagtgcaatg ttggtgatgc tggcttgttt ctatttcgtg gcccaaacca cccccgggct
      421 ctaaattgca gttggcagcc tgtaaaccct tcaaacccct gcgaaagggg ctggtattgt
      481 cttttgcggc tgttttcggc ttcagtggtt gttcggcaag caggcctgag tcttccacag
      541 cggtgactgg gatcgctgaa aggccggtgg gtcggctagt ccagccgggg aaggagagcg
      601 aagacaaaaa ctctcagctt gtgcttggca tggtctggga gccggtttcc tttaatccgc
      661 tgcgaggcat cgactcgggt tcttactgtg cttccagtct ggtctacgaa gggctggtca
      721 aattcgacga aaaccttgat ctggtgggtg gcctggccga gagcttcagc gtctctcaag
      781 atggtttgac atatcgattt cggctgcgac cgaatctgcg ctattcagac ggtgaaaaga
      841 ttgaagccgc tgatatcaag gcttcgcttc tgcttggtgc ctcaagcatt tcgccttttc
      901 gtagcgatta caaagacatg aaggaagtag ttattgagga cgagcgcaat ctggttgtag
      961 ttctggagag accatgccag cccttgcttt cccgtatggc ggaattgcgc atattgccga
     1021 cccatatctt gcagtcggca gatcatggca acaagcgcct gggacgtagc cccatttgta
     1081 ccggtgctta tcaactcaag cgttggaaag ccggacagga attggtattt gaacgcaacc
     1141 cctattactg gggcaggccg gccgaaacgg cctccataat ctggcgagtg ataccggata
     1201 aaatggcact gtcggccgca ctggccaggg gtgaagttga tctggcaccg gtagacggca
     1261 ggctctggca aaactttctc agtaagagta aagagcatct ggccatggct gagtttaatg
     1321 gtggacgcac tgtctatctt ggtttcaatt tacagaaagc accgtggaat aacctcaagg
     1381 tgcgtcaggc tttcgccagt gccatcaatc gtggtgccat ggtggatgct ttctacagtg
     1441 gtcatgcggt ggtgcccaat acggatgtgc ccctcacaag ctgggctttt gagccgaatt
     1501 taccgcgggc cgattttgca ccggagaaag taccggcgct tttgcaggaa gccggcttta
     1561 agaaggaagg caaggtctgg ttgaaagacg gcaaggtgct ggcgctgcgg attctcacta
     1621 tcaaggattt tgaagagatg gcgcaggcgg tggcagatta tttacgccgg gtggatattg
     1681 ctgctgaagt cgaggtaatg gaatactcga ctttgcgccg ggcttttatg caaaagggtc
     1741 tctttgatgt aattctctgg agtcggtcat ttggaccgga tccggagtgc tcaatggtat
     1801 ggagcagcgg cggtgctctc aatttctgtc gcttgcagga tgcccgggtt gacggatggt
     1861 tgcgggaagc caggctggcg ccgaacaggc aggcacggaa ggctatttac aggaagtttc
     1921 aggagtatct ggctactgag ctgccctggc tgtttctggc ccagcccagg cagataatgg
     1981 cgtacagaga aggtatggtc ggaatacaaa aaggccggca aaaagatgcc ggcctaccgt
     2041 gggacaacat tgccgccaac gccgcctttt ggaagcatcg gtaggtgttt atcccgcgta
     2101 tttgattgta tttagcctat tgccaacgcc tgccagagcg ctaaatgcat tcagaatgtc
     2161 aaaagtgaca aacccagacc aaccctcaac atgcctccag gacaagcccg gagccatggt
     2221 tttgaggatt cgtgtctacc agagaacgag ccttttaggg ctctatccta cttggtgcag
     2281 aggggcttat cgccgttgtg ctctctgtac caacgcaggg ctttggccca gcggtggatg
     2341 cactgttttc tgtgacggct aatggagtag gatggcggac ggaggtggaa atatcttccc
     2401 tcatcgccgc aaacctgcgc attcgctgcc ggcaccgaaa cggctgccag ggctgcaagg
     2461 actagaagtg ctgatctcat cgaacgtctt ctcctttcag aagttgcgcc aggtaacctg
     2521 gtaaacgatc tttagtttgg ttacagggca caccagggac actccgacga atgtcccatg
     2581 ggtgattgta ctattcccgg cccatctgaa aatatgtcaa gcgttcgttt gtaaaggtgc
     2641 gtattgccgt taaatggcaa gtggtggggc tttcaaggat ttcaaaaatt aatcttgtcg
     2701 ctttattatc agcccttggc aggcttgcaa atgccgtgca gtggcgcata atggtggatg
     2761 actcgggaaa gacatatcta ccggtggtgg catattgttt gctttacata tgctcccaga
     2821 acgtgacaac ggtctttgcc tgtcgaagat atcttctatt ttttccgttc agaaaatagg
     2881 agacggggcg gcatgttcag ggttacttga gcagcagttt ctaggattag catgtagagg
     2941 agtggttttt atcttctttc tttacttata tgattgaagt tcgcaatttt cttactagtg
     3001 ggaatataaa gttgtgcccc gactcaaagg ggcggcatgc ttggttgcaa agctataaca
     3061 agctgagcga ttgagaaaac gtagattaga tacaaagccg gcgattagaa ccaataccgc
     3121 gagaaagtag cagcgataca actggtgtca aaagcagtta gaagcgacca tgtgcccaat
     3181 agagtgccgc cccagcccgg tgagtgtcgg gatgcggtgc actggttcgt ggactccgtg
     3241 gggctgtacc agggctctcc cagagggctt attctctctg attattccga agaccaggaa
     3301 gctctcatag agtcggccgt gatcaatttc aagtcacgcg gcgtcaccag tatggtgata
     3361 gccggcgaca tgctttacaa gcaggccgat tatctttatc agcatgcagc agcccagacc
     3421 acttttggtc ggccgctctt atcaaagctt gaactggaca tgctcgacag cgaggtcttg
     3481 attgtcactg atctggtggc accggagacc ccgcagcagc tctggtattt ctatcatcac
     3541 ttgctttatc cccgtgctct cgctcagaag cctctggtga ttacgacacc actgggattg
     3601 gacgaatttt tcacttacgg cgccgaatgt gatgatctgg aatatgccgg acggcgcgtt
     3661 acctgggaaa aggccgcctg gctcatggac ggcatgctca tagatttgca ccacttccgc
     3721 atgttgcgtg ctgaaaatct gccgcccatg ttgaaggtgg agtacttctt atacaaggct
     3781 gtggtcagcc gcgggctcaa ccttttgccg cagcatgtga tgggcgacta tatggtcgat
     3841 tttgccctga tggaaagagg caacaagttg gccatagaat gcgatctgct ggccagtctc
     3901 gataatccta attcacgttc ggcagaagct acacgcactc tggtgctact gagcgacggc
     3961 tggaaactct tacgctttac cacggtggat ctgttggcca acttgatgga ttgcgtggat
     4021 gccgtggccg atgtctgggt gacggggcgc aaaaagtcac cttcggggag gctggtttcc
     4081 ggtcaatctt cctctcattt gctggagtta cctgttgaag acgacgtgga gaggcagtgc
     4141 atcacatccg gtgccggacc ggttgccatt actggtggtg ccggtacagg caagagcact
     4201 tgtgtggtgc ggcgagtggc gcatttgctt gcgcagggcg tcaatccgga acgcattctc
     4261 gtcatctctc attccaccga gagcgtcagg tcgctcaggc ttgatattga atcgattatt
     4321 gataagccca ctttgcaaaa actgcacttt cacagttgga acgagctggg attgaagctt
     4381 ctaaaagaga acaccagcgc cattaagcgc aagcctcccc tcaaggtcga gggtaatcca
     4441 cagcgcgtta ttcagcggtt gctcaccaag tttaagcgtg atctcgacca gctaactctt
     4501 gaactctccg aagagctgga ggagtttacg attgcttctt tgatctcgct ctacaaagca
     4561 aatctgatca cgcccaagca cgtgaaagaa agggccaaga ccagtgtaga cgagctggtg
     4621 gctaaggtct atcagggtta tgaagagcag cttcagaaga gcaacaagat tgatcgcgac
     4681 gacatggtca cccttgccgc tcagcttctg gccgacaatg cagacatcag agcccgctat
     4741 caatatcagt acgaattcgt gctggtagac gaatttcagg atgccaacgc cgcctcggac
     4801 ctgatggcgc gtatgttgtc cttgccgcag gacaatctat tcatcgccgg tgatgaagac
     4861 caatctatct acgaaagcaa aggggccttg cccaagattc tttcagaggt ttccctgcgt
     4921 ctgcccaatg cccgctgtta tgtgctcgaa aacaactggc gctcccaccc tgaaatagtg
     4981 gagctggcgg gccgtttgct ggagggactg agtcgtcgcc gcatccgcaa agatatggtg
     5041 cccggttggg agatgtccgg ggaacgtgcc gtgatcggac cgcagctggc cgagtcggaa
     5101 gcggcggaag cggaatgggt ggcccaggaa attcagggtt tgttgtcgga aggacgcaat
     5161 ccgcaagaca tagccatact gtaccggtac aaccgttacg cttcactggt ggaggaggca
     5221 ctctttcgca aaggtatcaa atgtctcacc actaatccag actctaccat ggtgcctgac
     5281 gaagtagccg acgtgatggc cttcttgcgc ctggttatgg acccggacgg tcccaaggcg
     5341 agagaatcgt tcgagcgcat ctgccagttg cgcaccaagg agattgaccc caagttattc
     5401 aataccatcg ctagtttcgc cgaggccaac aacctctctt tcttgaaagc ggtcgagatt
     5461 tactcggaag cggtggccga tcagtcttgc caagatctag aacagctggt gcgcattatt
     5521 cgcaccatga atcatgaaaa tctgccgccg gcggaaacca tcgcccttct caagcgcacc
     5581 cagaggctca gtgaatatta caaggccgtg aaagtgcctc cgggagttaa ctatgagcca
     5641 atgcgcaaac tttcgcagtt ggaggaagaa gcacgcaagt tcaaaaccgt aagcgaattt
     5701 gtgcgcagcc agaccagcca gaagtcggcc gacggtcagg cagagcctct ggtttatatt
     5761 ctcagtctgc acgaatccaa aggtaaggag tttccggtag tcttcctcac cggtatggcc
     5821 gagggactat tcccatcgga gacggctgga gatcccgagg aggagcggcg cctgtgctac
     5881 cttggcatgt cgagagccaa ggacatgctc tatgtttcgt atcccggcaa attcaatgac
     5941 gtggtgctgg cgccttcgcg ctttttgttg gaaaccatgc aggattccac acccaagccg
     6001 gtttctgctg ccggtgctac ggtgcaaccg ggggcggcat cggctgccgg tgccaccatg
     6061 caagcacagc aggaagcggc tctcaaggct caacaagagg cagccctgag agcgcagcag
     6121 gaagctgctc aaagagccca gcaagaagca gcattgaggg cgcagcagga agcggccctg
     6181 aaggctcagc aagaagcagc actgagggcg cagcaggaag cagcactgag ggcgcagcaa
     6241 gaggcagccc tgagggcgca gcaggaagcg gccctgaagg ctcagcaaga agcagcactg
     6301 agggc
//
LOCUS       DAOQNZ010000027         6283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4829_length_6283_cov_4.135356, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000027 DAOQNZ010000000
VERSION     DAOQNZ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4829_length_6283_cov_4.135356"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKW73_00880"
     CDS             complement(<1..329)
                     /locus_tag="PKW73_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71862.1"
                     /translation="MKVPPGRLLAIAALGSLCLAAGVAAAPTKPWERLKDQASILEVN
                     HHYSQSIELYKQALRLVPAADELCRLQILCALADCYKKVLDGENTCAVLDQIEVLAKT
                     MQTNGKLG"
     gene            447..1301
                     /locus_tag="PKW73_00885"
     CDS             447..1301
                     /locus_tag="PKW73_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA71863.1"
                     /translation="MTSKAYLTTRNQVRVPAIMYGTAWKKEQTTRLVKLALEAGFTAI
                     DSANQLKHYDEALVGQAISEFESQGVKRQDLFLQTKFTSLNGQDHRLPYDPSQPLTVQ
                     VRQSMESSLKHFNTDFVDSYILHGPQARYGLTAEDLEVWATMEKLYDEGKTRVIGVSN
                     VSFEQLSELCALARVKPMMVQNRCYAVMGWDAEVREICRKEEIIYQGFSLLTANTRVL
                     SHPGVHDIARRLKATLPQVIFRFAMQLSMLPLTGTSNRQHMQEDLLAEQFELTAQDMS
                     FIENLELN"
     gene            complement(1321..2628)
                     /locus_tag="PKW73_00890"
     CDS             complement(1321..2628)
                     /locus_tag="PKW73_00890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNA71864.1"
                     /translation="MTDLVRKSLNLAFVALLTVWTLGAGFFIFRVYLTGQTPLNPATG
                     FHSDYVRVFVLGKMMNQYGEKAVYDKVIHDRTMDEVVYPVKRTGCSYITYPPNVVFLF
                     SFISRIPLALGQVLWISFGIALSTLSLLLLRRTVSSSASWASDLFVVLSSFGSYSFFD
                     MCRLGQTTPYILSGTALCYYFARRGRPILAGLVTVLAAVKPQYCILWLIPLLAMRNWK
                     ALSTACAVHLLLLLATGFYCGFAIYPDYLKYAAGSNHSDLDNLRNIMITLGGLFTVFG
                     NPTLTNALSFLIFALGNLALYLLFTRCRRSSPEALVLCLSLSVLSGVLTNLYALWYDA
                     SLVTIVSALLISSPRIFGVSGSWLERAVNLMLLCFPIFSWLPLAVLVSLVKSPALTPT
                     MILSVSGKSICLYLAVLAVLCLLRTKAVGRFSQSAAASSSLGS"
     gene            complement(2646..2966)
                     /locus_tag="PKW73_00895"
     CDS             complement(2646..2966)
                     /locus_tag="PKW73_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNA71865.1"
                     /translation="MVLAERASVARSFFRRLKGLLGTDSLPDGEGLLLSPCNSIHMFG
                     MKYAIDVIFLDKELVVVDVLENIAPGKASRVYRKAYCCLELPAGKVNLTGTTIGDKLD
                     FRNP"
     gene            complement(3024..3314)
                     /locus_tag="PKW73_00900"
     CDS             complement(3024..3314)
                     /locus_tag="PKW73_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA71866.1"
                     /translation="MREMGERCGVDELINMASALIAAEAKGADMSYPLRQQADALRDR
                     LKRKKEEEAGKVPVKMVPVIMIFVMPLILCPMLGPAVVTIINAVGPIMAGTK"
     gene            complement(3336..3950)
                     /locus_tag="PKW73_00905"
     CDS             complement(3336..3950)
                     /locus_tag="PKW73_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71867.1"
                     /translation="MPTPVLAIMVFTCVVASCILVLRPVFGTYVDNYGVRLRPRKAQE
                     DTAAEKPKDSALLKLAELVGTFALQLMPALADKRTVQLLTMANYRTANHMAIFIGIKA
                     ILIGTVLTMTFLAAASNPVMLLLGLVGCIPGWILPNFFLAGRVKARQTDILRELPIII
                     DLLIVCAQAGLGLMLAVDKVSKEVTESCPILSVELQQLIADVKV"
     gene            complement(4019..4951)
                     /locus_tag="PKW73_00910"
     CDS             complement(4019..4951)
                     /locus_tag="PKW73_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA71868.1"
                     /translation="MQGPIIFSSIIVIGLLAMVLLRRDSVDQLARLTKQSRRMAEKMR
                     DNADPESIFVDQKTDYLSTLLARAGLEAQYDKMRMQWLYSAIGAGALGALGFILNGLP
                     EMSPIGFFLGVPMGAAGFVFYIGEIAKKRQAKMTEQLPQILETMVSALRAGSPVMEVF
                     KVISDTGPDPIKSEFKRGLISLQLGKPFRDVMKEMSLRIKAPDFKLLTQAIFISQDVG
                     GNLADVVATIAEAIRERFKLRDFLNALTSQGKATATFIGCLPYLITVGTYFLTPSYMT
                     PFLNHPIARIVMVGLVMWELLGFYILRRMVTFEV"
     gene            complement(5140..>6283)
                     /locus_tag="PKW73_00915"
     CDS             complement(5140..>6283)
                     /locus_tag="PKW73_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNA71869.1"
                     /translation="RIREIVNSDPAPLTMMEKGILLQNVLDEVFGFGPLGPLLRDPSV
                     GDICVNNVNSVYVERHGRLEKTSVVFENDRHLRQTIDKIIQPLGRRLDENSPMVDARL
                     PNGSRVNATIPPVTIDGPTITIRCFGTSIMSLGQLVEKGAMSVQMAEVLKSCVKGRIN
                     LIISGGTGSGKTTLLNALSAHINPRERIITIEDAAELRLQHEHWVRMETRPPNTEGRG
                     QITQRMLVINTLRMRPDRIILGECRGEEAFDMLQAMNTGHSGSMTTIHANNPRDCTKR
                     LENMILMCGMEMPQKAARELIASALQIIVQIKRLEDGTRRVTEIAEITGMEGDTIAIS
                     TMFALEREGRDPRGFFKCRHVGSGLPPKFLEQLEQEAVPFKLEWLR"
BASE COUNT         1675 a         1611 c         1694 g         1303 t
ORIGIN      
        1 ccgagcttgc cgtttgtttg catggtcttg gccagcactt caatttgatc aagcacggcg
       61 caggtgtttt cgccatccag cactttctta tagcagtcag ccagagcgca gagaatctgc
      121 aatctgcata gctcatcggc agccggtaca agcctgaggg cctgtttata gagctcaatg
      181 gactggctgt aatggtgatt gacttccagg atggaagcct gatctttcag gcgctcccag
      241 ggtttggtcg gcgccgccgc cacccctgcc gccaggcaca gactgcccag ggcagcaata
      301 gcaagcaatc ttcccggggg cactttcatg aaaacggtcg ctcaaattgc caatgaagct
      361 aaaataccag ttaatcagac tattctacct ttacggtgaa gcataagcct ctgacaacca
      421 gcgagctaaa cagaaacaag agcattatga ccagcaaggc ctatttaacc actagaaacc
      481 aggtgcgggt accagcaatc atgtatggca cggcctggaa aaaggaacag acgacgagat
      541 tggtgaaact ggcattagaa gccggattta ccgccataga cagcgccaat caactgaagc
      601 attacgatga agccctggtg ggacaggcca tttccgaatt tgaaagccag ggggttaagc
      661 ggcaggatct cttcctgcaa accaaattta cctccttgaa cggtcaggac caccgcctgc
      721 cctatgatcc cagccagccc ctgaccgtgc aggtcaggca atctatggaa agctcgctca
      781 agcacttcaa taccgacttc gtggattctt atattttgca tggtccccag gcaagatacg
      841 gtctgacagc cgaagacctg gaggtctggg cgaccatgga gaaactttat gacgaaggca
      901 agacccgagt gatcggggta agcaatgtct cttttgagca attgagcgag ctttgcgccc
      961 tggctagagt caaaccaatg atggtgcaaa accgctgcta tgccgtcatg ggctgggacg
     1021 ctgaagtaag agagatttgc cggaaagaag agattatcta ccagggtttc tcgctgctca
     1081 ctgctaatac cagggtcctc tcccatcccg gtgtgcacga catcgcccgg cgtctcaagg
     1141 ccacgctgcc ccaggtaatt ttccgctttg ccatgcaact gtccatgttg cccctcaccg
     1201 gcacaagcaa ccggcaacac atgcaagaag acctcctggc cgagcaattc gaactgacag
     1261 cgcaagacat gagctttatt gaaaatctgg aactcaattg agtctcaaag gtaatcgcac
     1321 tcaacttcca agcgaactag acgccgctgc cgactgcgaa aaacgtccga cggctttcgt
     1381 ccgaagcaaa cagagcacag ccagcaccgc taagtaaagg caaatgctct tgccgctcac
     1441 cgaaaggatc atggtggggg tgagagcggg cgacttgacc aaagacacaa gcacagccag
     1501 aggcagccaa ctaaaaatcg ggaaacagag gagcatgagg ttgacggctc gttccaacca
     1561 tgaaccactg actccaaaaa tacggggact ggaaataagt agagcagaca cgatagtgac
     1621 gagtgaagcg tcgtaccaga gggcatacaa attggtcagc acccctgaaa gaacactcaa
     1681 cgacaaacaa agcaccagtg cttccgggct actgcggcgg cagcgagtaa agagcagata
     1741 gagggcaaga ttaccaaggg caaatatgag gaagctcagg gcattggtca acgtaggatt
     1801 gccgaagacc gtaaagagtc cgcccagagt aatcatgata ttgcgcagat tatcgagatc
     1861 actatgattg gagccggccg catacttcag ataatcgggg taaatagcaa agccgcaata
     1921 gaagccagtg gcaagcagaa gcaaaagatg aacggcgcaa gcagtggaaa gcgctttcca
     1981 gttgcgcata gcaagcaatg ggatgagcca gagaatacag tactggggct tcactgccgc
     2041 caaaaccgta accagaccag ctaaaatggg ccgcccgcgg cgagcgaagt agtaacagag
     2101 ggccgtaccg gagagaatgt agggagtggt ctgaccgagg cggcacatat caaaaaaaga
     2161 gtaagagccg aaagaggaaa gcaccacgaa aaggtcactg gcccagctag cagagcttga
     2221 aacggtgcgc cgcaaaagca gcagcgacag ggtggaaagg gctatgccga agcttatcca
     2281 caagacctga ccaagtgcca agggaatgcg ggaaatgaag gagaagagaa aaaccacatt
     2341 gggtggatag gtaatgtagg agcagccggt gcgcttcacc ggataaacta cctcatccat
     2401 ggtacggtcg tgaataactt tgtcgtagac ggccttctcg ccgtactggt tcatcatttt
     2461 ccccaagacg aagacccgca cgtaatcgga atggaagcca gtggcaggat tgagtggggt
     2521 ctgtccggtc agataaactc gaaagatgaa aaagcctgcc cccaacgtcc agaccgtaag
     2581 cagagcgaca aaagctagat ttagagactt tctaaccagg tcggtcatat ttgtgctcat
     2641 caccgtcagg gattccgaaa atccaacttg tcaccgatgg tagtgccggt taagttcacc
     2701 ttcccggccg gcaattccag gcagcaatat gctttgcgat aaactcgcga ggcctttcct
     2761 ggagcgatgt tttccagcac atccaccacc accagttcct tatcgagaaa gatcacatca
     2821 atagcgtact tcatgccgaa catgtgaatg ctattgcagg gagaaagcaa aagtccctca
     2881 ccgtcaggca agctgtccgt acccaatagc cctttcagcc gcctaaagaa agagcgcgct
     2941 actgaagcgc gctctgctaa gaccatatct ttagtgagat tggtgaaccg gcaagtactt
     3001 ccgtctgcag gcagcttcaa gacttacttt gtaccagcca tgatcggacc gacggcgttg
     3061 atgatggtta caacggctgg tcccaacatc ggacacaaga tcaaaggcat aacgaagatc
     3121 atgattaccg gtaccatctt aaccggtacc ttaccggctt cttcttcctt cttacgcttg
     3181 aggcggtcgc ggagagcatc ggcttgctgg cgcagaggat agctcatgtc ggcacccttc
     3241 gcttcagcag cgatgagagc ggacgccatg ttgatcaatt cgtcgacacc gcatctttca
     3301 cccatctcac gcagcgcata gggtactgtt ttagcttaca ccttaacgtc ggctatgagc
     3361 tgctgcaatt caacggaaag aatgggacag gactcggtga cctctttgga aactttgtct
     3421 acggcaagca tcaaaccaag accagcctgg gcacatacga tgagcaagtc gatgatgatc
     3481 ggcaactctc tcaagatgtc ggtctgtctg gccttaactc gaccagccag gaagaagttg
     3541 ggcagaatcc aaccgggaat acagccgacc agacccagga gcaacataac cgggttggaa
     3601 gcggcagcca ggaaggtcat ggtgagaacg gtaccgatca agatggcttt gataccgatg
     3661 aagatggcca tgtgattggc ggtacggtag ttagccatgg tcagaagctg gacggttctc
     3721 ttatcggcga gagcaggcat caactgcagg gcaaaagtac caaccaactc tgccagtttc
     3781 agcaaggcag aatctttcgg tttctcggcg gcagtgtctt cttgcgcctt ccgcggtctt
     3841 aagcgcactc cgtagttgtc cacgtaagtg ccgaagaccg gacgcaacac caaaatgcag
     3901 cttgcgacca cacaggtgaa gaccattatg gctagtactg gtgttggcat tggagtctag
     3961 tttcctcaaa gagtacttga ataactatgg aatcaagagc tggttagccc ggggtcgcct
     4021 aaacttcgaa ggtgaccatg cgtctcagaa tatagaagcc gaggagctcc cacatcacaa
     4081 gacccaccat tacgattcta gcaataggat ggttcaagaa gggggtcata tacgaaggtg
     4141 tcaagaagta agtacctacc gtaatcaaat aaggcaggca tccgataaag gtggcggtag
     4201 ctttaccctg agaagtcaga gcgttgagga agtctcttag cttgaatctt tcacggatag
     4261 cttcggcgat ggtagccaca acgtccgcca ggttgccccc tacgtcttgc gagataaaga
     4321 tggcctgggt gagaagtttg aagtcgggtg ccttgatgcg cagggacatt tctttcatca
     4381 catcacggaa cggtttgccg agctggagag aaataaggcc tctcttgaac tcagacttga
     4441 tgggatccgg gccggtgtcg gaaataactt tgaagacttc cataaccgga gaaccggctc
     4501 tcagcgcgga caccatggtc tccagaatct gaggcaattg ctcggtcatc ttagcctggc
     4561 gtttcttggc gatctcaccg atgtagaaca cgaaaccggc ggctcccatg ggaacgccca
     4621 ggaagaaacc aatcggcgac atttccggca gaccgttgag aatgaaccca agagcgccca
     4681 gtgcacctgc tcctatggca gagtagagcc actgcatgcg catcttgtca tactgagctt
     4741 ccagaccggc gcgagccagc aaggtagata agtagtcggt cttctggtca acgaagatgc
     4801 tctccggatc ggcattatcg cgcatctttt ctgccatacg gcgcgactgc ttggtcaagc
     4861 gcgccaactg gtccactgaa tcacggcgca ggagcaccat ggccagcagg cctatgacaa
     4921 ttatggagct gaaaattatt ggaccttgca tgtcagatat ctcaaatcaa aagggaccgg
     4981 ctatttttcg aaatctagtt tgtggtcagc cgaccgttca gtcagccagg tggaaaaacc
     5041 agcacattct tggggcattc agcagagaaa acggaaattg agaagggatg gaattccact
     5101 ttggatggaa attgccaacc ctcctcaaaa gtccaagctt tatctgagcc actccagttt
     5161 gaagggcacg gcttcctgct caagctgctc gaggaacttg ggcggcaaac cggaaccaac
     5221 gtggcggcat ttgaagaagc ctctcgggtc acgaccttca cgctcaagag cgaacatggt
     5281 ggagatggcg atggtgtcgc cttccattcc ggttatctcg gcgatctccg tgacacgacg
     5341 ggtaccgtct tccagacgct taatctgcac aatgatctga agagctgagg cgatcaattc
     5401 gcgggcagct ttctgaggca tttccatacc gcacataagg atcatgtttt caagacgctt
     5461 cgtgcagtca cggggattgt tggcgtggat tgtggtcatg gaaccgctgt gaccggtgtt
     5521 catggcctgc aacatgtcga aggcttcctc accacgacac tcaccaagga tgatgcggtc
     5581 gggtctcata cgcagggtgt tgataacaag catgcgctgt gtgatctgac cacgaccttc
     5641 cgtgttcgga ggacgggtct ccatgcgtac ccagtgctcg tgctgcaaac gaagttccgc
     5701 cgcgtcctca atcgtgatga tgcgttcgcg ggggttgatg tgagcagaaa gagcgttgag
     5761 cagggtcgtt ttaccggaac ctgtaccacc ggagatgatc aggttgatac gacctttaac
     5821 gcaagacttc agtacctcag ccatctgtac gctcatggcg cccttctcga ccaattgacc
     5881 gagggacata atggacgtac cgaagcatcg gattgtgatc gtcggaccgt cgatggtgac
     5941 cggcgggatc gtggcgttaa ccctggaacc attgggaaga cgggcgtcaa ccatgggaga
     6001 gttttcgtca agacgacgac cgagaggctg aatgattttg tcgatggtct gacgcaagtg
     6061 tctgtcgttc tcgaaaacca cgctggtctt ctcaagacga ccgtgacgtt ctacgtagac
     6121 gctgttcacg ttgttgacgc aaatatcgcc gacgctcggg tcacgcagca agggaccgag
     6181 aggaccgaaa ccgaacactt cgtcgagtac gttctggaga aggatacctt tctccatcat
     6241 ggtcagagga gccgggtcgg agttgacgat ttctctgata cga
//
LOCUS       DAOQNZ010000028         6264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4845_length_6264_cov_3.668062, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000028 DAOQNZ010000000
VERSION     DAOQNZ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4845_length_6264_cov_3.668062"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..746
                     /locus_tag="PKW73_00920"
     CDS             114..746
                     /locus_tag="PKW73_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA71870.1"
                     /translation="MSLIQEIFPVGPLGCNCSIIGCSETGEALVIDPGGDVDDIISRL
                     TELGLKPKYLLHTHAHFDHIMGSRGMREKTGASICLHKEDQFLYDMLGKQASMFGLSA
                     DEPLPVDKFLSDEEEVSFGKYKTAVLHTPGHTPGSLCFSVQEKDSLLFSGDTLFSRSI
                     GRTDLWGGSFDKIIKSIKDRLLCLDDSTRVIPGHGPDTDIWTEKNENPFL"
     gene            760..1926
                     /locus_tag="PKW73_00925"
     CDS             760..1926
                     /locus_tag="PKW73_00925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNA71871.1"
                     /translation="MNYRTDEIDFSPFNADEVLKALCSVLRTSKHLSFSYAGDIQSLI
                     DRIGRTFYAARSMIFLSGSDDGQQVEVFEYVKRGKTAVAHEFASPFGQKMARHLLTLP
                     QEVHLVEDVDKFSEEIDEGFRDMFKKIMEAVGKERVYLIPLRLLPPDSAGTKGPERRA
                     GLLLLQEIDSSSQTKWNKLVVESLVTIADHLAKLFEVESLRGRLEAHESEDPATGFLN
                     RRGGVLAITEEIERSRFFGDRLSLMLIHVDSAKKSDAPMGAAQAKAVLTAVSGVVSSQ
                     SRPVDVICRFGHDEYIVALPRMSREEAVRVAQQIKSSVGAALLNVLEKGKQEKHGQSY
                     DGDQEGLKSQPLTVSIGIASMPEDGNKFDDLLSACHELISEAKCMGGNQVKSRS"
     gene            2056..2946
                     /locus_tag="PKW73_00930"
     CDS             2056..2946
                     /locus_tag="PKW73_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNA71872.1"
                     /translation="MTIEKSHWHLGGILAWLAFCLAVLALLASPIYFFASLLAVDHWT
                     KALSLLVGPFGLFLVSIVILRGFAVIQPNDALILMFYGSYCGTVKRNGLVLVNPFCSR
                     GRISLKMQNLHGDILKVNDKRGNPIEIGAVVVWHVAEPARAMFDVDSYELFVRIQSEA
                     AVRHLANMYAYDGDGEDTTLRSGVDEVSHTLKAELEQRLSRAGIAIDEARIAHLAYAP
                     EIAMAMLKRQQAEAVISAKRAIVSGAVSIVTSALQELQDARLVDLDEERKAAMVSNLL
                     VVLCSEGQVQPVVNTGTLYS"
     gene            2976..3179
                     /locus_tag="PKW73_00935"
     CDS             2976..3179
                     /locus_tag="PKW73_00935"
                     /inference="COORDINATES: protein motif:HMM:NF023331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71873.1"
                     /translation="MARKSFLIRIDEKLFQDIESWAQQEMRSVNGQIEYLLRQACLRY
                     RGREVGESGVIKVDAVHDAELLD"
     gene            complement(3187..4896)
                     /locus_tag="PKW73_00940"
     CDS             complement(3187..4896)
                     /locus_tag="PKW73_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71874.1"
                     /translation="MRSPIHKVNEMQKRAKRGSVLIVILLTAISMVLCLYFAVDLGVG
                     ITGARQSHSAIEAAALAAAQELSAVVIKDPYFGYIALSDRPPTEEHLRGADGEPCPVT
                     STNTLIASARLQMLIALALENELMQEMARRDLAQARRAAGELEKHLGEALSKNCKNKY
                     QDSNGREIKPYAVAEAVFRRTLEGKDRADSISEKQELRLELGYCPGTGTTVTPLPEPP
                     GQKPISARFNTTVNYQPSKNYPLGGEDFYLAAVGPQASLVGTKTFSRPDGKHISSIVR
                     ASTTRQVSSLARQDLSMALNQAACAQPAAEGQATTAGSLLVDLPAGKISNFQTLFDLI
                     AARPDTAFPLPVLSPQQGDYPDEAAPPVLEGGTACMTARELFSVGLYDWLRTAGARID
                     PDSVINAVKNEKLFEAQTNGAKRFTRFDLGVDGKVYITRGSENPFNSTRVNEKQLYCF
                     SSNAFKTGQGECTLVFTDQVHRLGPTQGGRHAGMPAPSPTVNWVDLSTYCGNVDLADR
                     LGRGHKLGLDARGERSKLNDDGIAADGAYYERPLRGRLANCPHKSYYSGGLAVCLQLM
                     EER"
     gene            complement(4932..>6264)
                     /locus_tag="PKW73_00945"
     CDS             complement(4932..>6264)
                     /locus_tag="PKW73_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71875.1"
                     /translation="IPQRAVANPNHQALPCLDFKRSCPMKLTKKYIQSNKLALALSAA
                     LVLTLAGASLVEAKSLYVSKAGNNADGLTWQSAWNELDQIDWNKVELNDEIFIDGGAY
                     GGSMTYRKPLIIGKGAQPMSGGFIIISAGQGLDHAGTAVINGLNKTNFYGIQASGKRN
                     VVFRSYGRRGWLKVTNCTTGVYTKDGSQNYFQGLEIANCGVGAHLEGDSVALVQANIH
                     DNIKNVEIHNPGASVGTQSPSGLYACWIYNGPQAVPPGNAAGITVTAGAVPPVSGNPS
                     GNAVINNCVLGPNLMQGVDNGFPGLKINGCLFLNAYSSSLTLRNSTEFDRCTLFVTAR
                     NSLGNPKTAIRVLAGNNSIRNSIIYGGVVRIPQGLSYGPKNTQFATTGNTSTLSAGLV
                     DPLFVSPVGTYQEPVSFAVLKNANFALKPGSPAQGTGAQVTSVQAVLNQLQ"
BASE COUNT         1316 a         1570 c         1795 g         1583 t
ORIGIN      
        1 ccccacgaac ccggcaccgg ctcacccaag attgtgcagc tttccgtggc caagctgctc
       61 ggtgaagcga tctggcgtat tcacgacgac agttcggtta gcgagatgtt cgaatgagct
      121 tgattcagga aatctttcct gtcggtcctc tgggctgtaa ctgctctatc atcggttgtt
      181 ctgagacggg cgaagcactt gttatcgatc cgggcggtga tgtggatgac atcatcagtc
      241 gcctcaccga actgggtttg aaacccaagt atctgctcca tacccatgcc catttcgacc
      301 acatcatggg tagtcgcggc atgagggaga agaccggcgc ctccatttgc ctgcacaagg
      361 aagaccagtt tctctacgac atgctcggca agcaggcttc tatgttcgga ctttctgccg
      421 atgagcctct gcctgtggat aaattccttt ctgatgaaga ggaagtcagt ttcggaaagt
      481 acaagaccgc cgtgctgcat actcccggtc atacgccggg cagcctctgc tttagcgtcc
      541 aggaaaagga tagtctcttg ttttccggcg acacgctttt tagccgctcg attggacgca
      601 ccgatctctg gggtggttcc ttcgataaaa ttatcaagtc aatcaaagac aggctccttt
      661 gcctcgatga tagtacgaga gtgatacccg gtcatggacc ggacactgat atttggacag
      721 agaaaaacga aaatcccttc ctttgagatt tggctcgcct tgaattacag aaccgacgaa
      781 attgatttct cgccctttaa tgccgatgag gtactgaaag cgctctgttc tgtcttgcgc
      841 accagcaagc atctctcctt cagctatgcc ggcgacattc agagcttgat cgatcgcatc
      901 gggcgcactt tctacgccgc ccgctccatg atctttcttt ccggctccga tgacgggcaa
      961 caggtggaag tttttgaata tgtgaaacgc ggtaaaacgg ctgtggctca cgaattcgcc
     1021 tcgcctttcg ggcagaagat ggcccgtcac ctgctcactt tgccccagga agtgcatctg
     1081 gtggaagatg tggacaagtt ttcggaagaa atagatgaag gctttcgaga tatgttcaag
     1141 aagataatgg aagccgtggg caaggaacgt gtctatctga ttcctttgcg gctcttacca
     1201 cccgatagtg ccggtaccaa aggtccggag cggcgcgccg gtctactgct attgcaggaa
     1261 atcgattcca gttctcagac caagtggaac aagctggtgg tggagagcct ggtcaccatt
     1321 gccgatcatc tggccaaact gtttgaagtt gagagtttga ggggccggct ggaagcccat
     1381 gaaagtgaag atccggccac tggttttctc aatcgccgcg gcggcgtgct ggccataacg
     1441 gaagaaattg aacgctctcg attcttcggg gacaggcttt ctctcatgct tattcatgtg
     1501 gatagtgcca agaagagtga cgcgcccatg ggagccgctc aagccaaggc ggttttgact
     1561 gcggtatcgg gtgtggtatc aagccagagc cgtccggtag atgtaatctg tcgctttggc
     1621 catgatgagt atatagtggc cttgccccgc atgagccggg aagaggctgt gcgcgtagcc
     1681 cagcagatca agagttcggt gggagcggcg cttctcaatg ttctcgaaaa aggaaagcaa
     1741 gaaaagcacg gccagtccta tgacggcgac caggaggggc tgaagagcca acccctgacc
     1801 gtcagcatcg gtattgcttc tatgcccgag gacggcaaca aattcgacga tcttctctcc
     1861 gcctgtcacg aactcatttc cgaagctaag tgtatgggcg gcaaccaggt caagtcgcgc
     1921 agctaaggca gtcggcgcac tacatgtccc cggggtggtg gctccggcgg ggtcttgtca
     1981 tctgttatca atgtgatatc attgtgatat gacattgcta gcaggtctgc ttccatcaat
     2041 ataggtttcg cgactatgac catagaaaaa tcccactggc atctaggcgg catcctggcc
     2101 tggcttgcct tttgtctggc ggttctggct ctgcttgcca gtcctatcta tttttttgca
     2161 tcgcttctgg ccgtcgacca ttggactaaa gcgctttccc tgttggttgg tccattcggg
     2221 ttgtttcttg tttctattgt tatactgcgc ggctttgcag taattcagcc caatgacgct
     2281 ttgatcttga tgttctacgg cagctattgc ggcacggtga agcgtaatgg tctggtgctg
     2341 gtcaatccct tttgctcaag aggcagaatt tctctaaaga tgcagaacct gcacggggat
     2401 atccttaagg tgaacgacaa acgcggcaat ccaattgaaa ttggggccgt cgttgtctgg
     2461 catgtggccg aaccggcccg ggccatgttc gacgtagata gttatgaact cttcgtgcgc
     2521 atccagagtg aggctgccgt tcgccacctg gccaatatgt atgcctatga cggcgatgga
     2581 gaggatacca ccctcagaag cggtgtcgac gaagtttcgc acaccctgaa agcagagttg
     2641 gaacaaagac tctcaagagc cggaattgct atcgacgaag cgcgcattgc tcatcttgcc
     2701 tacgcgcctg agatcgccat ggccatgctg aagcggcagc aggcggaagc ggttatctcg
     2761 gccaagcgtg ccatcgtatc cggtgcggtg agcattgtca ccagcgcctt gcaggaattg
     2821 caagatgccc gactggtaga tctcgatgaa gagcggaaag cagccatggt cagcaatctg
     2881 cttgtggttc tctgcagtga aggacaggtg cagccggtgg tgaacaccgg tactttatat
     2941 agctagagcc gcgagtttgg aacgaggatg ttttggtggc ccgtaaatct ttcctgatta
     3001 gaatagacga gaagcttttt caggacatcg agtcctgggc tcaacaggag atgcgcagtg
     3061 taaacggtca gattgagtat ctcttgaggc aggcctgctt gcgctaccgg ggacgggaag
     3121 tgggagagag cggtgtcatt aaggtggatg ccgttcacga cgcagagctt ttggactgag
     3181 taaggtttag cgttcttcca tcagttgcag gcagactgcc agcccgcccg agtagtagga
     3241 tttatggggg caattggcca acctgcctct gagcgggcgt tcatagtagg ctccatcagc
     3301 ggctatcccg tcgtcgttca gcttactcct ctcgcctctg gcgtcgagac ccagtttgtg
     3361 tccacgacct agcctgtcgg ccagatcgac gttgccgcaa taggtagaca aatccaccca
     3421 gttgactgtc ggcgacggcg cgggcatgcc ggcatggcgt cctccctgtg ttggtcccag
     3481 gcggtgtacc tgatccgtga aaacgagggt gcattctccc tgtccggtct tgaaggcatt
     3541 ggaagagaag cagtagagtt gtttctcatt tactctcgta gagttgaagg gattttccga
     3601 gcctctggtt atgtagactt tgccgtctac tcccaggtcg aatctggtga atcgtttggc
     3661 gccgtttgtc tgcgcctcaa agagcttttc gtttttgacg gcattgatga cggaatccgg
     3721 gtcaattcta gctccggcag tgcgcagcca gtcgtagagc ccgacgctga agagttctcg
     3781 ggcggtcatg caagccgtgc ctccttccag aaccggagga gccgcttcgt ctgggtagtc
     3841 accttgttgc ggtgatagaa ccggcaaggg aaaggcggtg tctggtctgg ccgcaattag
     3901 atcgaagagt gtctggaagt tggaaatttt tcctgccggt aggtctacta gaagagaacc
     3961 ggccgttgtt gcctgacctt cggctgccgg ttgcgcgcaa gcagcctgat tcaaggccat
     4021 agataggtcc tgtctggcca gggaactgac ttgcctggtg gtgcttgctc tcacgatgga
     4081 tgaaatatgc ttaccgtccg gtctcgaaaa agttttggtg ccgacaagag aagcctgggg
     4141 acccactgcc gccaggtaaa agtcttcccc gcccagcgga taattctttg atggctggta
     4201 gttgaccgta gtattgaatc ttgctgaaat cggtttttga cccggtggtt cgggcagggg
     4261 ggttacggtt gtaccggtgc cagggcaata accaagttca agccgtaatt cctgcttttc
     4321 agaaatgctg tcggctctgt ctttcccttc cagggtccgg cgaaaaactg cctcagcgac
     4381 tgcgtagggt ttgatttcgc ggccgtttga atcttgatac ttgttcttgc agttttttga
     4441 aagtgcttcg ccaaggtgct tctccagttc cccggcggct cttctggcct gagccagatc
     4501 tcgcctggcc atctcttgca taagttcgtt ttccagggcc agggcgataa gcatttgcag
     4561 gcgggccgag gctataagag tattggtgga agtgaccggg cagggctcac cgtcggctcc
     4621 gcgcagatgc tcttccgtgg gcggcctgtc gctcagtgcg atgtagccga aatagggatc
     4681 tttgattacc actgccgaaa gttcctgggc tgctgccagg gccgcggcct ctattgccga
     4741 atgtgattgc ctggcgcctg tgatgcccac gcccagatca acggcgaagt agaggcaaag
     4801 caccatgctg atggcggtaa gcagaattac tatcaatacg ctgcctcttt tggctcgctt
     4861 ttgcatttcg ttcaccttat ggatgggaga cctcattgaa aattcaaacc tcaatgaggt
     4921 ctcctttgtt tctactgaag ttgattgagc acggcttgca cggaagtaac ctgagcgccg
     4981 gtaccctggg ccggtgaacc tggtttgagg gcgaagttgg cattcttcaa aacggcgaag
     5041 gaaacgggtt cttgataggt gcccacgggg ctgacgaaga gtggatccac cagtccggca
     5101 cttaaggtgc tggtgttgcc ggtggtggca aattgggtgt tcttcggtcc atagctcaag
     5161 ccctgcggaa tgcgcaccac accgccatag atgatggaat tcctgatgct attgttgccg
     5221 gccagaactc tgatagcggt tttgggattg ccgaggctat ttctggctgt gacgaagagc
     5281 gtgcaccggt caaactccgt gctgtttctc agggtgagag aggatgaata ggcgttgaga
     5341 aagaggcagc cgttgatttt cagtccggga aagccgttgt ccacaccttg catgagatta
     5401 ggtcccagta cacagttatt gatgacggca ttgccggaag gattacctga tacaggcggt
     5461 acagcgcctg ctgtaactgt aatgccggct gcattgcccg ggggcactgc ctgcggccca
     5521 ttgtaaatcc agcaggcgta aagaccggat gggctctgtg ttcccactga ggcgccggga
     5581 ttgtgaatct caacgttttt tatattgtcg tgaatgtttg cctggacgag ggctacactg
     5641 tctccttcaa ggtgggcacc aactccgcag tttgcaatct ccagcccctg aaagtagttc
     5701 tgagacccgt ccttggtgta gacaccggtg gtgcagttgg ttactttcag ccagccgcgg
     5761 cgaccataac tacgaaatac aacatttctc ttgccgcttg cctggatgcc gtagaagttg
     5821 gttttgttca gtccgtttat cacggctgta ccggcgtgat caagaccctg cccggctgag
     5881 ataatgatga agcctccact catgggttgg gctcctttgc ctatgatgag aggcttacgg
     5941 taagtcatgg agccgccgta ggcgccgccg tctataaaga tttcgtcgtt tagttccacc
     6001 ttgttccagt cgatttgatc aagttcgttc caggccgact gccaggtgag accatcggcg
     6061 ttattgccgg cttttgatac atagaggctt ttggcttcta ctaaactggc gcctgcaagt
     6121 gttagaacca gggccgcact gagtgccagg gctaatttgt ttgattgaat gtatttctta
     6181 gtaagtttca taggacagct ccttttaaag tcgagacaag gcagagcctg gtggttgggg
     6241 ttggccaccg ctcgttgggg tatg
//
LOCUS       DAOQNZ010000029         6262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4848_length_6262_cov_3.545191, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000029 DAOQNZ010000000
VERSION     DAOQNZ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4848_length_6262_cov_3.545191"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PKW73_00950"
     CDS             <1..1671
                     /locus_tag="PKW73_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71876.1"
                     /translation="QVEVKILDFGISELTEAAPIQGSTLEHSAHVVESTQARLDSNDA
                     KPERAKTQRNQLEIIGSALYVSPEQLLGDNLDRRSDIYTIGCTMFETLTGAPPFLEES
                     FEKLREAHLHQEPPTLGQATGLFFPPKITAIVAKCLEKEPEKRYQSANELSIDLERAL
                     QGKELQFARLNAGQALETTEAAGTRPARIMLAAFTVVISTCLASAALCWMAISLMPSA
                     STETVGKQSEKLTGQGAHSQGASTANQPIAPTVEVIPNQKGGYSVQLTPEVNASDLLL
                     LDLGTLTTKAIPSDTKVLDTTANEKLVLYPLVSHINAFYKSGDLEAEIRGLDLTRDRN
                     AAGTLMLYAGEVFPKLQYMRVCGGSQQEATARFREVAHLKDLEALTYVGETKTTAKLS
                     APAGLKKLTLENYRLPLVSLDTSQTRHLSKTTTLKELMKDENYLHQKQSTPKAIIFKN
                     SQLKGSSFRFLIDHKPPDQVVLEDCRLDESPFEDMAVYGINKVTVLMHTETLPPWLSP
                     ESLKKNPIAMPTLVLINPTWTAARCREYEAQVKSRCPGFKCSASNKPE"
     gene            1783..2133
                     /locus_tag="PKW73_00955"
     CDS             1783..2133
                     /locus_tag="PKW73_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71877.1"
                     /translation="MLLKSRQAVRPALKQDTYRQGDVLLQKVTQLPDNALETECEGFV
                     ILQFGEVTGHKHAISTNFAKLFTAKGQRYIEVQTGAQLVHEEHGTINLPQGYYRVIQQ
                     REYVPEAPPRDVVD"
     gene            2120..3427
                     /locus_tag="PKW73_00960"
     CDS             2120..3427
                     /locus_tag="PKW73_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71878.1"
                     /translation="MSSTDKIDKLNAKHLRLLEKVREEWRQVGLGTGATDRSKAEAAV
                     KEAYQAIGLKPPAIFVWLKSPWAGNAAAKILDSDIDWPYHLNNNQLEVWDAVWKQCVK
                     QVEAAEGEEKWKSVRRRLRAEADEKVLAREGVLLEKYVKDQFAENMGIYIWHYLRNFA
                     GVSMSRRLREDAEEKVKENIEGKLSALAAEQIYHELVQPVHMQVWSFLAEPLKQRIPS
                     MSGILAGTQRWELNYGLHDASWLSYYDFIGRIGIKGTEPLSGLSKLARVGGWIWAYEN
                     ICIMTERPLELVRDNRFRLHAENKMCLRYADDWGLYAWHGVLVPPYVILLPEPLTFDL
                     IESEPNAEVRRVLIERFGLENYLREGKVLRIHQDKAGILYRMNLPSDEPILVVRVMNS
                     TPEPDGTIKEYFLRVPPTMQRAKQAVAWTFGLTEEEYDPLVET"
     gene            3741..3977
                     /locus_tag="PKW73_00965"
     CDS             3741..3977
                     /locus_tag="PKW73_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71879.1"
                     /translation="MNGSTSTSNSPKEANSSKASFNNGKGDITSGSFSGNLFDIWQGQ
                     LSRQDQNQKQLKDRKTTISELIKPVNSTKFLRHH"
     gene            4013..4174
                     /locus_tag="PKW73_00970"
     CDS             4013..4174
                     /locus_tag="PKW73_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71880.1"
                     /translation="MIDNEIFSLIILSLILWLLSDDIKIGLREAGIDFIKHKPQAAVV
                     RIARNDGHR"
     gene            4264..4872
                     /locus_tag="PKW73_00975"
     CDS             4264..4872
                     /locus_tag="PKW73_00975"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNA71881.1"
                     /translation="MKSVATRIDTRCLLIEAGAKVMLSKGYNNTGIQEIVKLVGVPKG
                     SFYHYFESKEAFALAVIEKFDQAHRSKLTKSLGDKSRSSIDRLTHYFQAGADELTNSP
                     CRGGCLIGTLSQEMSGQSELLREKLALVMDGWKAEFAACLKEGQEKGEIRRDLCPNQL
                     AELVLSGFEGAVLRSKATNSPAPLQSFQGLMPRLLTESSVGA"
     gene            5046..5750
                     /locus_tag="PKW73_00980"
     CDS             5046..5750
                     /locus_tag="PKW73_00980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNA71882.1"
                     /translation="MSPEKKQEERFTRDSLFKDKLSDIRRAEQKKTEETRKVLNAQSQ
                     LANTKTILPPEEETPTGKDRHHLLKDPKTGLYSERFIKRVLKEEVKRAKRYKNPLGLV
                     VGTIVNLEEIAKSGGPIAKSALEVEAAKVLQECIRDVDVPGVSNDGTYIVICPETGEV
                     GLHVIIERLLKRLNYNQKINLKTTWEPVFCFSASAFGTSLNSADFLLAGALERLNLLA
                     LETKAEMDNTAKEEKW"
     gene            complement(5780..>6262)
                     /locus_tag="PKW73_00985"
     CDS             complement(5780..>6262)
                     /locus_tag="PKW73_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71883.1"
                     /translation="GQKETTAFLFALGLVRALGISAACWFDNLKSVAYCAIPIVLVAA
                     LVLPKLKLTPSHFYEVGKRPSFSWRKLALAVTLGLMAGSLGVFTSLLQHDLSHMSERV
                     IRLGLTMAMTSAIVSSLIPLIEWYADNVPGRTMGYIGASLFVLGFMVQAIPSLVAVLK
                     "
BASE COUNT         1730 a         1769 c         1588 g         1175 t
ORIGIN      
        1 caggtagaag taaaaatcct cgacttcggc atttccgaac tgacggaggc cgccccgata
       61 cagggttcta ctcttgagca cagcgctcat gtagtcgaga gcacacaagc cagactcgac
      121 tctaacgacg ccaagccgga gagagcgaag acccagcgca atcaactgga aattatcggc
      181 agcgccctct atgtgagccc cgagcaattg ctgggtgata atctcgatag gcgctccgac
      241 atctacacca tcggttgcac catgttcgag accctgaccg gcgcgccgcc ctttctggaa
      301 gaatctttcg aaaaactgag ggaagcgcac ctgcatcaag agccgcctac gctgggtcag
      361 gccaccgggc ttttcttccc gcccaaaatt acggccatcg tagctaaatg cctggagaaa
      421 gaacccgaaa agcgctacca gagcgccaat gagctatcta ttgatcttga gcgcgccctg
      481 caaggcaaag agctacaatt cgctcgcttg aacgccgggc aggctctgga gaccacagag
      541 gcggccggca ccagaccggc acgaatcatg ctagccgctt tcaccgtcgt catttcaacc
      601 tgcctggcca gtgcggccct ttgttggatg gctatttctt tgatgccttc ggcgagcacc
      661 gaaactgtcg gcaaacaatc tgaaaagctc actggccagg gcgctcactc acaaggggcg
      721 agcaccgcca atcagcccat tgcacccact gtagaggtga tccccaatca gaagggcggc
      781 tattcggtgc aactgactcc ggaagtaaat gcttccgatc tgctgctact ggatcttggc
      841 accctcacca cgaaagcaat tccctctgat accaaggtgc ttgataccac agccaatgaa
      901 aaactggttc tttatcccct ggtcagtcat atcaatgcct tctacaaaag cggcgacctg
      961 gaggctgaaa ttcgaggctt agacctgacg cgcgatcgaa atgcagccgg taccctcatg
     1021 ctctatgccg gggaagtatt tccaaaactt cagtacatgc gtgtctgcgg cggctcacag
     1081 caggaagcga cggcgcgctt ccgcgaggtg gcccacctca aggatctgga agcgctcacc
     1141 tatgtgggcg aaacgaagac gacggcgaag ctgagtgctc ccgccggttt gaaaaaactt
     1201 accctggaaa actaccgtct gcccctggtc agtcttgata caagccaaac cagacattta
     1261 tccaaaacca ctacactgaa agaattgatg aaagacgaga actatttgca tcaaaaacaa
     1321 tccaccccaa aagcaatcat cttcaaaaac agccagctga agggctcatc cttccgcttc
     1381 ctcatagacc acaaaccgcc cgatcaagtc gtgctggaag actgccgcct ggacgagagc
     1441 cccttcgagg acatggccgt ttacggcatc aacaaagtga ctgtgctcat gcatacggaa
     1501 accctgccgc cctggctcag cccagagtct ctcaagaaaa acccgatcgc catgcccacc
     1561 ctggtgctca tcaatcccac ctggaccgcc gcccgctgcc gggagtatga ggcacaagtg
     1621 aaaagccgct gcccggggtt caagtgctcg gcctcgaaca agccggaata gtttacaacc
     1681 ggtaaaagtc ggctgttccg gttcaaagaa gggcaaaact gggtagaatg aaaaatagac
     1741 ggaatttgcg gacatccgtc acctacgcca gcaggtttta ctatgcttct taaatcaaga
     1801 caggcagtgc gccccgccct caagcaggat acttacagac agggcgatgt gctcttgcag
     1861 aaagtgacac agctaccgga taacgccctt gaaaccgagt gcgaaggctt tgtcattctg
     1921 cagtttggtg aagtgaccgg acacaagcat gccatcagca caaattttgc caaacttttc
     1981 acagccaaag ggcagcgcta tattgaagtg caaaccggag cccagttagt gcacgaagag
     2041 cacggcacca tcaacctccc tcaaggctac taccgcgtca tacagcaacg cgagtacgtg
     2101 ccggaagcac cgcctagaga tgtcgtcgac tgacaaaatc gacaagttaa acgccaagca
     2161 tctgcgcctg ctcgaaaaag tgcgggaaga atggcgacag gtcggtcttg gcacgggtgc
     2221 cactgaccgc tccaaagccg aggcagccgt gaaggaagct taccaggcca tcggtctgaa
     2281 gccgccggct atcttcgtct ggctcaaatc gccctgggca ggtaatgctg ccgccaaaat
     2341 tctcgacagt gacatagact ggccctacca cctcaacaat aaccaactgg aagtctggga
     2401 cgccgtctgg aagcaatgcg tcaagcaagt agaggcggcc gagggggagg aaaaatggaa
     2461 gtcggtgcgc cgcaggctgc gcgccgaagc cgatgaaaag gtactggcgc gcgaaggcgt
     2521 cttgctggaa aaatatgtga aagaccagtt tgccgagaac atgggcatct atatctggca
     2581 ctacctgcgc aactttgccg gcgtctccat gtcgcggcga ctgcgggaag acgcggaaga
     2641 gaaagtcaaa gaaaacatcg aaggcaagct ctctgccctg gctgccgagc agatttatca
     2701 cgagctggtg cagccggtgc atatgcaagt ctggagcttt ctagcagaac ctctcaaaca
     2761 gcggattccc tccatgagcg gcatattggc cggcacacaa cgctgggaat tgaattatgg
     2821 cttgcatgat gcaagctggc tctcgtacta cgattttatc ggcagaatcg gcatcaaagg
     2881 caccgagccc ttgagcggct tgagcaaact ggcgcgggta gggggctgga tctgggccta
     2941 cgaaaacatc tgcatcatga cggagcgacc tctggagctg gtgcgcgaca atcgcttccg
     3001 cctgcacgct gaaaacaaaa tgtgcctgcg ctacgcagac gactggggtc tctatgcctg
     3061 gcacggggta ctggtgccgc cctatgtaat tctcttgccg gagcccctaa cttttgactt
     3121 aatagaatcg gaacctaatg cggaagtgcg gcgcgtattg atcgagcgct tcggtctgga
     3181 aaactatctg cgcgaaggca aggttctgag aatccatcaa gacaaagccg gcattctcta
     3241 tcgaatgaat ctgccttccg acgaacccat tctggtggtc agggtaatga actccacccc
     3301 tgagccggac ggtaccatca aagaatattt tctgcgggtg ccgcctacca tgcagagagc
     3361 caaacaagcg gtggcctgga ccttcggctt gaccgaagaa gaatatgatc cgctggtgga
     3421 gacctagaac cttaattgag cctgcctgat tgccttctgt gggagaaatt atggcgaatt
     3481 tcgctcgcgc ctcgaaaata gaagccggat agcccatggc tcatccgacc cacctgcccc
     3541 aatcatttca cctgcgccat tcatttcgcc tgccaacttc agtcactccc gctaacccag
     3601 cgccattgaa cgcagagcaa gtttgattta tggggtactt gccaatttag agacgaccgg
     3661 tctattatag agtgcatgga caacccaagc caaacgaatt tccaacccgc aaaggcacaa
     3721 taaagccgga ggtaagacca atgaacggaa gcacaagcac tagcaactcg cccaaagagg
     3781 caaacagcag caaagccagt ttcaacaacg gcaaaggcga cataaccagc ggcagttttt
     3841 ccggcaacct ctttgatatc tggcaggggc aactgagccg gcaggaccag aaccaaaagc
     3901 aattgaaaga tagaaagacc accatcagcg aacttatcaa acctgtgaac agcacaaaat
     3961 ttttgcgcca tcactagacg accggtcaat tcatttataa ggaaccaaaa tcatgatcga
     4021 taacgaaatc ttctctctca tcatcctctc gctaatactc tggctgcttt ccgacgacat
     4081 caaaatcggg ctgcgggaag ccggcataga tttcatcaag cacaagcctc aagcagccgt
     4141 ggtgagaatc gccagaaatg acggacaccg ctaaaaccca atccacaacc ccggcgccag
     4201 gcgtcgtcaa ttcaacccga gcgccgtaac cagaccaatc taacaacaat aggaccaaga
     4261 gccatgaaaa gcgttgccac cagaatcgat accagatgcc tgctcattga agcaggggcc
     4321 aaagttatgc tctccaaggg ctacaacaac acaggcatcc aggaaatcgt caagctggtg
     4381 ggggtgccca aaggttcttt ctaccactat ttcgagagca aagaagcttt cgccctggcc
     4441 gtcattgaaa aattcgacca ggcccatcga agcaagctca ctaagagcct cggcgataaa
     4501 agtcggtcga gcatagacag actgacgcac tatttccaag ccggtgcaga tgaactgacc
     4561 aacagtcctt gccggggcgg ctgcctgatt ggcacgcttt cgcaggaaat gtccggtcaa
     4621 agcgaactac tcagggaaaa attggcgcta gtgatggacg gctggaaggc ggaatttgcc
     4681 gcctgcctca aagaaggtca ggagaagggc gagattcgcc gagacctctg ccccaaccaa
     4741 ttggcggagc tggttttgag cggcttcgaa ggggcggtac tgcgcagcaa agccaccaat
     4801 agccccgctc cgctgcaatc cttccagggc cttatgccca ggctactgac cgagagcagc
     4861 gtcggcgcct gatggaagct caggcatgct caaatagcca gaggcgaaac tatattagaa
     4921 acgaatatta aagcccacac tacaggtaaa ccatggcaaa ataggcagat atacgacagc
     4981 ccaccatgac atcctggtag ggcgccgttc agccagtgaa gaagcccagg aagtagagaa
     5041 aaaccatgtc gccagaaaag aaacaagaag aacgctttac cagagactca ctcttcaaag
     5101 acaagctgtc cgacatacgc agagccgagc agaagaagac agaagagacc agaaaggtct
     5161 tgaatgctca gtcacaactg gccaacacca agaccatctt gccccccgaa gaagaaacgc
     5221 ccacaggcaa agaccgtcac caccttttga aagatccgaa aaccgggctc tattccgaac
     5281 gcttcatcaa acgcgttctc aaagaagaag tgaagcgcgc caagcgttat aaaaatcccc
     5341 tcggcctggt ggtgggcaca atcgtcaacc tcgaagaaat agcaaaaagc ggcggaccca
     5401 ttgctaagtc ggctctggaa gtggaagcgg ctaaagtctt gcaggaatgc atccgcgacg
     5461 tagatgtgcc gggagtttcc aatgacggca cttatatcgt catctgtccc gaaacaggcg
     5521 aagtaggctt gcatgtcata atcgagcgcc tgctcaagcg cctcaactac aatcaaaaga
     5581 tcaatctcaa aaccacctgg gaaccggtct tctgcttctc agcctcagct ttcggcacca
     5641 gtttgaacag cgccgacttt cttctggccg gtgccctgga gcgcttgaat ctgctggccc
     5701 ttgagaccaa agccgaaatg gataacaccg ctaaagaaga gaagtggtaa atagcgaagc
     5761 ctgcaaagat tagccggcat cacttcagca ctgccaccag tgatggtatc gcctgcacca
     5821 taaatcccag cacaaaaagc gaagcgccta tatagcccat ggtgcgaccg ggcacgttat
     5881 cggcatacca ttctattagc ggaatgagcg agctgacgat ggccgaggtc atggccatgg
     5941 tcagtcccag ccgaatcacg cgctcggaca tatgactcaa atcgtgctgc aataaagaag
     6001 taaatacgcc caggctaccg gccatgagac cgagagtcac ggccagcgct agcttacgcc
     6061 aactaaatga cggtcttttg cccacttcgt agaagtgtga cggcgtcagt ttcagtttcg
     6121 gcaaaaccag cgccgccact agaactatgg gaatagcgca gtaagcgacg ctcttcaagt
     6181 tatcgaacca gcaggcggct gaaataccca gagcccgcac cagtcccagg gcaaaaagaa
     6241 aagccgtggt ctctttctgc cc
//
LOCUS       DAOQNZ010000030         6259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_4851_length_6259_cov_3.312057, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000030 DAOQNZ010000000
VERSION     DAOQNZ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4851_length_6259_cov_3.312057"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..2352
                     /locus_tag="PKW73_00990"
     CDS             109..2352
                     /locus_tag="PKW73_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA71884.1"
                     /translation="MTKSIARQPSYSKGKSREFTSELYLKSEQLLRFRIAFSLVSLCL
                     CVGLYSFAGGEFDFYRATGIICGVSIYSFVGLAFVVKNLKASSRKLQAFNAVLLFLDV
                     LALTGLVHFTHGLESDLYVLYLLPIMLSSYTFGRLGIYLVSTLVTLSYIGLLMLESSP
                     LLPYLFHNSAFGNGLTSGESGATSGLASAFALKLWRRIFARAFFFASVAFLWARFCDY
                     LSRVSRRSNERLLEQLEANEKLVIESEERAKRERLINSINKGVRSTLELDSILEAAVE
                     QLSQAVPAHLVAILCPPTLDVKDDDGGRRMKDPLYVQAAAPAADSAGVGSGKGIAPVL
                     QNFFLRRRSSYVKDPETGAKIKTFIYQSPARDQFFADVQEELLETGYKTLVLQPMMYG
                     EVSKGIVILADCRADRNFSESELVLTKVVAGQVAVAIEHANLVGELSFKNRDLVGKNL
                     NLDAKNLELKTMQSQLIHHEKMASLGRLVAGIAHELNNPINFVHGNLPYLKQYVDELK
                     RLVEAAGEIPEEHRKPLEELKRKLKYDFLVSDLDNIIADLNEGSERIRHIIRNLRSFS
                     RLDEAELKEASIAEGIESTLKILSQYYGKDKIPVNFKEGFAELPQVLCYPGQLNQVWM
                     NLLSNAAQAMQGVEGASLDIEARLEGESVLVQITDHGPGIKPEVQSKIFDPFFTTKPV
                     GQGTGLGLSICHSIIERHGGQIWFTSEISKGTTFYVRVPLTARRQAGGDGYSAASVTA
                     DYLSDPD"
     gene            2398..3528
                     /locus_tag="PKW73_00995"
     CDS             2398..3528
                     /locus_tag="PKW73_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA71885.1"
                     /translation="MRRHKILVVDDEVPNLRLLRRVLSEEHDIFEAASGQEGVNLLEK
                     EEISLIITDQRMPSMTGVQLLEQSLKIRPDAIKILLTGYTDVQALIDAINAGHVYKYI
                     PKPWDADELKLTVRRALEAFELKERNDNLVIELSGALTELENLSVGTIRALADALDAK
                     CDYTAGHSLRVSRIAVLIGRQMGLPEDTLRDIELGGILHDIGKIGVPESILWKPDKLT
                     AEERSIMSRHPVKSAEIIGDLKGLVRAREYVKHHHEFFDGSGYPDGLEGENIPIGARI
                     ILVSDAYDAMTTDRPYRKAIGHVRAIEELRKLAGTQFDPVVVTNLLSLTEDGGKEMDK
                     IIAEQFDESFIGLTLASVDPAKTGRQYREEAERRARLAGSEP"
     gene            complement(3437..4603)
                     /locus_tag="PKW73_01000"
     CDS             complement(3437..4603)
                     /locus_tag="PKW73_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNA71886.1"
                     /translation="MSLQPLPKKILTINFGGIGDEILFLPTLLEVKKLLPDSRISLLL
                     EPRSKSVKEVTDLVDEVLPFDIKKRPLLPSDYLKLLSLTRQGKYDLVISSGSSPMVAM
                     LLFASGIPVRIGYDAGSPVKCLLTHPVKLNKEQYAGNMYHDLSKGLRAYLDEHHKGQT
                     FRVDDGEGLLQIPRVTIDRAASARMKEALQIEERLLKQRLSLAPAGGGTGAVEDEREP
                     VQRILIHPGTSRLAVQKGIIKTWDSQNWLTLIRSLLAQKSPLKPIEIILAGGPDDEEV
                     MKGLMQALSSEADKVINFYGKTRSLADLAALIEITDLFICVDSAPMHVAVGLDKKVVA
                     LFGPTDPAKLIPKQKNFCALSDNASTARSLLDGLGVRLLPDTVVQSSLDLLRQG"
     gene            4722..6020
                     /locus_tag="PKW73_01005"
     CDS             4722..6020
                     /locus_tag="PKW73_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNA71887.1"
                     /translation="MKLSHWLFIFITCALLGALGAYLYVSVSKPQLMPPQLRIATLKR
                     PCTILLLGTDVVYSDSGRRLKADKDAFTGRSDTILVGRLDPIANTLRVISIPRDTVVD
                     IPGNGSQKINAANALGGPTLAVSTVSQFLDTPIDHYVVLNVHGLVDLVNELGGITVEV
                     PKKMQYMDWTAKLKIDLEPGVHTLTGNQAMGFVRFRHDALGDIGRVQRQEIFLRAVLD
                     KALKPESWRHIPRLMEIAQQYISTDLTVPQMMEMANFVRGVPKSNQLLTMMPGSFSPS
                     GDWAVSQSDVRRMVSRLMGANFIETTRDDIRIVIGNSSSTEGMGFKLARILGSKGYKN
                     VIVQKRMLELSDPVKRTRIIAQKANPEDAGLVKADLGNIGDVVNASVGDIESSVTIII
                     GDDLASKILSSEAAQSSQEGRAESASGAGSGQPVTGAVTH"
BASE COUNT         1401 a         1596 c         1715 g         1547 t
ORIGIN      
        1 ccctatgatg ccgacgtcta cgatgcctgg ggcacggcgc tcaagaccct gggtcgcttt
       61 accgaagccg ctgaagtata caagcgcgcc tccgagtaca tttagtctat gactaaaagc
      121 atagcccgtc agccgagcta cagtaaggga aagagccggg aattcacttc cgagctgtat
      181 ttaaagagcg aacagttgtt gcgcttccgc attgcctttt ccctggtcag tctctgtctt
      241 tgcgtcggtc tttatagttt cgccggcggt gaattcgatt tctaccgtgc tactggtatc
      301 atctgtggtg tgagcatcta ttccttcgtc ggtctggcct ttgtggtgaa gaacctcaag
      361 gccagcagtc gcaagttgca agccttcaat gccgtcctgc tcttcctgga tgtgctggcc
      421 ctcaccggcc tggtgcactt cacccatggg ctggagtctg atctttatgt tctctatctt
      481 ttgccgatca tgctctccag ctacactttc ggccgcctgg gcatttatct ggtttccact
      541 ctggtcacgc tttcctatat cgggcttttg atgctggaaa gttctcctct cttgccttat
      601 cttttccaca attctgcctt cggcaacggt ctcacatcgg gagagagcgg cgccacaagc
      661 ggtctcgctt ccgcctttgc gctcaagctc tggcgccgca tctttgccag ggccttcttc
      721 tttgcttcgg tggccttcct ctgggcccgt ttctgcgatt atctgagccg tgtatcaagg
      781 cgcagcaatg agcgcctgct tgaacaattg gaagccaacg agaagctcgt tatcgaatcg
      841 gaagagcggg ccaagcgaga acgtttgatc aattccatca ataagggtgt gcgcagcact
      901 ctggaacttg attccatatt ggaagcggcg gtggagcagc tctcacaggc ggttccggcc
      961 caccttgtgg ccatcctctg ccctcccacc cttgatgtga aggatgacga cggtggtcgt
     1021 cgcatgaaag accctctcta tgtgcaagcc gcagctcccg ctgctgattc cgccggtgtc
     1081 ggctctggta aagggatcgc gccggttttg cagaacttct ttttgcggcg gcgcagttcc
     1141 tatgtgaagg atccggaaac cggcgccaag atcaaaactt ttatctatca atcaccggcc
     1201 agggatcagt tctttgcgga tgtgcaggag gaactgcttg aaaccggcta caagaccctc
     1261 gttctgcagc cgatgatgta cggcgaggtc tccaaaggca tagtgattct ggctgattgc
     1321 cgggctgatc gtaatttttc ggaatcggag ctggtgctca ccaaggtggt ggccgggcag
     1381 gtggctgtag ccatcgagca tgccaatctg gtcggtgagc tttccttcaa gaaccgcgat
     1441 ctggtcggta agaatctcaa cctggatgcc aagaacctgg agctcaagac catgcagtct
     1501 cagctcattc accacgaaaa aatggcatct ctgggtcgtc tggtggctgg tatcgcccat
     1561 gagcttaaca atcctattaa cttcgtgcac ggcaacctgc cctatttgaa gcaatacgtt
     1621 gacgagctga aaaggcttgt ggaggccgcc ggcgagatcc cggaggagca ccgaaagccc
     1681 ctggaggagc tcaagcgtaa gctcaagtac gacttcctgg tcagcgacct ggacaatata
     1741 attgccgact tgaatgaggg ctccgagcga attcgccata ttattcgcaa tctgcgcagc
     1801 ttctcccgcc tggatgaagc ggagctaaaa gaggcgtcca tagccgaagg tattgagtcg
     1861 accctcaaga tcctctccca gtactacggt aaagacaaga ttccggtcaa tttcaaggag
     1921 ggttttgctg aactgccgca ggtgctctgc tatcccggcc agctcaatca ggtctggatg
     1981 aacctgctct ccaacgccgc ccaggccatg cagggggtgg agggcgccag cctggatatc
     2041 gaggctcggc tggaggggga aagcgtgctt gtgcaaataa ccgaccacgg gcctggtata
     2101 aagcccgagg ttcagagtaa aatctttgat cccttcttca ctaccaaacc ggttggtcag
     2161 ggaacaggtc tcggtctgtc catttgtcat tcgataatcg aaaggcatgg cggacaaatc
     2221 tggtttacca gcgagatctc aaaaggcact accttttatg tgagggtgcc cctgactgcc
     2281 agaaggcagg ctggcggaga cgggtattcg gctgcttcag tgactgccga ttacttgagc
     2341 gatccagatt agagaaaccc gtccggcacg agggttcaaa agaggtattt gctagaaatg
     2401 cgccgtcaca aaatcctcgt agtcgatgat gaagttccta atctcaggct tttgagacgt
     2461 gtactttcgg aagagcacga tatttttgag gcggctagcg ggcaagaagg cgtcaatctg
     2521 ctggagaagg aagaaatttc cttgatcatc accgaccagc gcatgccctc catgaccgga
     2581 gtacagcttc tggagcagtc tttgaagatt cgcccggatg ccatcaagat tttgttgacc
     2641 ggctacaccg acgtacaggc gctaattgac gccattaatg ccggtcatgt ttataagtat
     2701 attcccaagc catgggatgc cgatgagctg aaactgacag tccgtagggc tcttgaagct
     2761 ttcgagttaa aagagcgcaa cgacaacctg gtcattgagc tttccggcgc cctcaccgag
     2821 ctggagaatc tctccgtggg cacaatcagg gccctggccg atgccctgga tgccaagtgc
     2881 gattacacgg ccgggcacag tctgagagta agccgcatag ccgtcttgat cggccgccag
     2941 atgggcctgc ccgaagacac cctgcgagac attgagctgg gcggcatctt gcatgatatc
     3001 ggcaagattg gcgtgcccga gtctattctc tggaagcccg acaaactgac ggcggaagag
     3061 cgctcgataa tgtcgcgcca cccggtcaag tctgctgaaa ttattggtga tttgaaaggg
     3121 cttgtgcgag cgcgggaata tgtcaagcat caccatgagt tcttcgacgg ctccggctac
     3181 ccggacggtt tagagggtga gaacattccg attggcgcgc gcatcattct ggtctcggat
     3241 gcctatgacg caatgaccac cgatcgcccc tatcgcaaag ctatcggaca tgtgcgtgct
     3301 attgaggaac tgcgcaagtt ggccggtacc cagtttgacc cggtggtggt aacaaacctt
     3361 ttgtctctca ccgaggatgg tggcaaggaa atggacaaga tcattgccga gcaattcgat
     3421 gagagcttta tcggtcttac ccttgcctca gtagatccag cgaagactgg acgacagtat
     3481 cgggaagaag ccgaacgccg agcccgtcta gcaggctccg agccgtagag gcattatcgc
     3541 taagggcgca gaagttcttc tgtttgggta tgagcttggc cggatcggtg ggaccgaaga
     3601 gtgccactac ttttttgtcc aagcccacgg ccacatgcat gggggccgaa tctacgcaga
     3661 tgaagagatc ggtgatttcg atgagggcgg caaggtcggc caggctccgt gttttgccgt
     3721 aaaaattgat tactttgtcg gcctcgctgc tgagagcctg catcagacct ttcattactt
     3781 cttcgtcatc ggggccgccg gcaagaatta tttctatggg cttaaggggg ctcttctgcg
     3841 ccagcagcga gcggattagt gtcagccagt tctggctgtc ccaggttttg atgatgcctt
     3901 tctggacagc cagtctggat gtgccggggt ggattaaaat tctctgtacc ggctcacgct
     3961 catcttccac agccccggtg ccaccaccgg caggggcaag gctgagcctt tgcttgagca
     4021 gtcgttcttc tatttgcagg gcctctttca tgcgtgcgga agcggctcga tcaatggtca
     4081 cgcgaggaat ctgcagcagt ccctctccgt cgtctacgcg aaaggtctga cctttgtggt
     4141 gttcgtccag gtaggctctc agacctttgc tcagatcgtg gtacatattg ccggcgtact
     4201 gttctttatt gagcttgacc ggatgggtga gcaagcattt gacgggagag ccggcgtcat
     4261 atccgattct gacgggaatg ccggaggcga aaagcagcat cgccaccatc ggcgaagagc
     4321 cggaggaaat aaccagatcg tacttgccct gcctggttag tgaaagcaac ttcaggtagt
     4381 cggagggtag gagcggacgt ttctttatat cgaaggggag aacctcgtcc accaggtccg
     4441 tcacttcctt cacggatttg gagcggggtt ccaggaggag ggaaatgcgg ctgtccggaa
     4501 gcagtttctt aacttccagc aaggtaggta gaaacaaaat ttcatccccg atgccgccga
     4561 aatttattgt caggattttc ttggggagag gctgtaggct cattctgtaa gtctcatttt
     4621 gttcagtcta tccatgggtt tggcctcgat tggtcagccg tctcatgatt aaacagctac
     4681 agcacgtata atatcccaga gtttggggac ccgtagatag cttgaaacta tcccattggc
     4741 tttttatctt tatcacttgc gccctcctag gagcgctcgg cgcctacctt tacgtatcgg
     4801 tatccaagcc tcaactgatg ccgccgcagt tgcgcatagc tactttgaag cggccctgta
     4861 ccatactttt gcttggtacc gacgtggtct attccgactc cgggcgcaga ctcaaagccg
     4921 ataaagatgc ttttacaggg cgctccgata ccattttggt agggcgcctt gatcctattg
     4981 ctaatacctt gagggtgatt tccataccgc gagatacagt ggttgatatc cccggtaacg
     5041 gttctcagaa aataaacgcg gccaatgccc tgggtggacc gactctcgct gtcagcacgg
     5101 tcagccagtt tctcgataca ccgattgacc actatgtggt tttgaacgtg cacggcctgg
     5161 tggatctggt caatgaactg ggcgggatta ccgtcgaagt tcccaagaag atgcaatata
     5221 tggactggac cgccaagttg aagattgatc tggagcccgg cgtacatacc cttaccggca
     5281 atcaagccat gggttttgtg cgttttcgcc atgatgcttt aggggatatc ggccgggtac
     5341 agaggcagga gatcttcctc agagccgtgc tggataaggc gctcaagcct gagtcctgga
     5401 ggcatattcc gcgcttgatg gaaatagcgc aacaatatat ctctactgat ctgacggtgc
     5461 cgcagatgat ggagatggcc aattttgtgc gtggcgttcc aaaatctaac cagctcttaa
     5521 cgatgatgcc cggcagcttt tcaccatccg gggattgggc cgtcagtcaa agtgatgtca
     5581 gaaggatggt atcgcggttg atgggtgcaa attttatcga gaccaccagg gatgatatca
     5641 gaatagttat tggcaactcc agctctaccg aaggaatggg ctttaaactg gctcgtatac
     5701 tgggttctaa gggctataag aatgtcattg ttcagaagcg catgcttgaa ttatcagacc
     5761 cggttaagcg cacgagaatc attgcgcaga aggcaaatcc cgaggacgcg ggtttagtaa
     5821 aagctgatct gggcaatata ggagatgtgg tgaatgcatc tgttggagat atagaaagct
     5881 ccgtcacgat catcatcggt gatgacctcg ccagtaaaat tctctcatca gaagcggcgc
     5941 agtcatccca ggaaggtaga gcggaatcag catcaggagc cggatctgga caacctgtaa
     6001 cgggagctgt tacacactaa gacagcaatt gttcagacaa tattattttt cactttgaaa
     6061 catagctccg cacttagtat ttcctggtgt acatttgagg caattgcgat taaactcctg
     6121 gtgaggcatt cttatacttc tctcaggtct caaataaact cttctttttc ttctcggcgt
     6181 acttcaaagg catctaagag caatctagaa aggcaggcgg catgattcat tacgtttatt
     6241 cgactcgcgg cggttatct
//
LOCUS       DAOQNZ010000031         6089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5059_length_6089_cov_3.157607, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000031 DAOQNZ010000000
VERSION     DAOQNZ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5059_length_6089_cov_3.157607"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2734
                     /locus_tag="PKW73_01010"
     CDS             <1..2734
                     /locus_tag="PKW73_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71888.1"
                     /translation="NVHSKPNWSSHVAQDKLSPRLTRPLSALCLALALLIPGPGPFLL
                     GQKWPGGENCPAGRLAKLGSSQAALAANPARAGAGSTAGSRGAISTGAMSGSPRAISP
                     AARVPGARSALSNSNLGLNSPIEDKWAVVIGISDFIDPSVPHLKYASKDARDFYDYLI
                     DPNGGKFSRDHVKLLLNSDASKVNILDTLGDSFLPHAADSNDLVLVYLSTHGSPSGAD
                     LDGINYLIAYDTQVRKLFATGIELNEIVQKIKKRVHTKRIVLVLDTCYSGAGAQGHKG
                     MYRTNANVQAVSQGIGSVVISSSTANQKAWESDDLRNSYFTRYLIDTLKEGGGTYDID
                     KVFNTMRSKVQSSVLKDKGEVQTPIMAGIFAGPPLNLAVPPSVTKEAPLTVPTSEGPA
                     RATAGSKVGTGAIDLTAYGEHIRLGNKLAQENKVWDAIHEFELAGKANPGTVEGYIVL
                     SRLYDSQGRFNEMLDNARRAVVNDKESSQGREALAMANLRLNNTDEAMRQVQLAISLD
                     PFNSMAHNLYGYINEFKFNRVDQAEKEYRKALELNPLNTRALVNLGRLLEVHHQNYDE
                     AESLFKKAIASDDDDFEARTALGHLLYSARNNYKEAEKEFRKAIEHSPANPRLHSELG
                     HVLSADKARLEEAEVELKKGIELGSGQSAAVPNYLMANFLWRKRGRIQEAERDYRKAL
                     EADPQYFEAMVALADLLVQHKNVYNESNDLYLKALKISPKNAEAHLGLALIKERLFKD
                     YGAAESELRAALAINPRYSQAADALGQLQELSLGKFSDARISYKDAVKMDPNNADAWL
                     HLGLINWQKFQESKTARTELEKAAGMETGKSVYRTALATLLITEFKDYKEAEKLLTQS
                     CQQNFADSEAHYRLGMLLIEKFGKRKAGEAELKIAYEQNPRDKDIKAAYERFVR"
     gene            2776..3303
                     /locus_tag="PKW73_01015"
     CDS             2776..3303
                     /locus_tag="PKW73_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HNA71889.1"
                     /translation="MPRNEFDPKFDDDDEDDDDFLDEEDLEERESEKQGRSFDLEELI
                     AAWEDDSPDNLYYFDTTTGAVKMVNRNLFDLRELTDEIEKNKERYLYLPKPDAKQLKG
                     DLRDFQKSIEDQSLKRVLDMAFESPHLLSSFKVILKSRELVGTLEHFLRSRSCLRIRQ
                     WMDANALTDRWQIEG"
     gene            complement(3447..3659)
                     /locus_tag="PKW73_01020"
     CDS             complement(3447..3659)
                     /locus_tag="PKW73_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71890.1"
                     /translation="MNTPVTVLLMGGEQVGGEGAMIDQSTYLCLRREGETLYLPWSAI
                     KCVRGPALPERGMGSNLPQGRYTTTT"
     gene            complement(3897..5759)
                     /locus_tag="PKW73_01025"
     CDS             complement(3897..5759)
                     /locus_tag="PKW73_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71891.1"
                     /translation="MAATPDVSKPTDATKPESKSWYETGFTGAIWDFGKSTFQSVGDV
                     TYSIYKGSGLESLYNSTKEKFSPIWDSYSDEFATSLKDDGKKVSDVVSALKNENGVVV
                     ETVKKDGAVDSCKIGDANTCWSGSVNLKKFEHTTADGVSIVKDGRNYEISLKDGDKVN
                     ITNDRGGVKDQIITKTNGDTVRRLQDGTYELYDKANDITTRVKNSQELEKTYGQTFAY
                     AARLAEDSRDVSRDYFRQIREGRAPGDPGILNRDNRLGVEDYRNGKGVNNGESIAVMS
                     QSNELTIIKKDGSGGARISGDQVQLLDAHGEPIPGRTISTREFLQQNANGFGGWRINA
                     DSSNLEAVDVEGRGRIRLNRATEQVTASQTATTGVDAGKTVQSATNGDDKRVVEFKGA
                     DDKPIQTTTIDPRNPERGVVEQTAGEQGHRVFDMSNPADIKYKEYTSQDENPKTLKYE
                     LGEHSSYFDGATVNHDTGTVYYDSPSGGRYTVYSSSPESYAASTSAANSASSHGSSVA
                     SQVNAAVSAGNFTSDAYRGLAIGAIANVDAAIQQCMVTGNFEGFGNLISTKDRLINAL
                     NSLNDKSNKFDDASKLGLSTEQAKYVAENTGNGSLHKAIEEVKTRANGRKPESW"
BASE COUNT         1541 a         1627 c         1566 g         1355 t
ORIGIN      
        1 taatgtgcat tccaaaccca attggagttc tcacgtggcc caagacaagc tctcgccccg
       61 actgacgcgc cccctgtcag cactctgcct ggccctggca ttgctaatac caggtcccgg
      121 tccgtttctg ctcgggcaaa aatggccggg cggagagaat tgcccggccg gtcgcttggc
      181 gaaacttggc tcatcacagg cagcgctggc cgccaaccct gccagggccg gtgccggttc
      241 cacagccggg tccagagggg ctatttccac cggtgcaatg tcaggctcgc ccagagccat
      301 tagcccggcg gccagagtgc ctggtgccag atcggcgcta agtaacagta acctggggct
      361 gaacagtccc atagaagaca aatgggcagt agtaattggc atttccgact tcatagatcc
      421 ttccgtaccg cacttaaaat acgccagcaa ggatgcgcga gacttttacg actatttgat
      481 tgatccaaac ggcggcaagt tcagccgcga ccacgtcaag ctcttgctca acagcgatgc
      541 cagcaaggta aacattctgg atacgctggg agactccttt ctaccccatg cggcagacag
      601 caacgacctg gttctagttt atctatccac ccacggttcc ccatccggcg ccgacctgga
      661 cggtatcaat tatctgattg cctacgatac tcaggtgcga aagctttttg ccaccggcat
      721 cgagctcaat gaaatcgttc agaaaatcaa gaaacgagtg cacaccaagc gcatcgtact
      781 ggtactggac acttgctata gcggcgccgg cgcccagggt cacaagggaa tgtacaggac
      841 aaatgccaat gtacaagcgg tttcccaggg tatcggttcg gtagtgattt cttcttccac
      901 cgccaatcag aaagcctggg agtcggatga cttgaggaac agctacttca cgcgctatct
      961 gatcgatacc ttgaaagaag gcggcggcac ctacgacatc gacaaggtgt tcaacaccat
     1021 gcgcagtaag gtgcagtcct cggtcctcaa ggacaaaggc gaggtacaga caccaattat
     1081 ggccggcatc ttcgccggtc cccccttgaa cctggcagta ccgccctcgg tgacaaagga
     1141 agcaccgctg accgtaccga cttccgaggg gccggccaga gctacggccg gctctaaagt
     1201 cggaacaggt gcaatcgacc tgactgcata tggggaacac atccgcctgg gaaacaaact
     1261 ggcgcaggag aacaaggtct gggacgccat tcatgaattt gaactggccg gcaaggctaa
     1321 tccgggcacg gtagaaggct acatagtgct ctctcgcctg tacgacagcc aggggcgatt
     1381 caacgagatg ctcgacaatg ccagacgagc ggtggtcaac gacaaagagt cttcccaggg
     1441 gcgtgaagct ctggccatgg ccaatttaag actgaacaat accgatgagg ccatgcgaca
     1501 ggtgcaactg gctattagtc tggacccctt caattcgatg gcccacaacc tgtacggcta
     1561 catcaacgaa ttcaaattca accgggtaga ccaggccgaa aaagagtatc gaaaggcact
     1621 ggaactcaat ccactcaaca cgagagcgct ggttaacctg gggcgcctgc tggaagtgca
     1681 ccaccagaat tacgacgagg ccgaaagtct attcaaaaaa gccatagcca gcgacgatga
     1741 tgactttgaa gcgagaacag ccctgggaca ccttctctac agcgccagaa acaactacaa
     1801 agaggcagaa aaggaatttc gcaaggccat tgaacattct ccggctaacc cacgtttgca
     1861 tagtgaactg ggtcatgtac tgtcagccga caaagcacgt ctggaagaag cggaagtgga
     1921 gctgaagaag ggtatcgagc ttggtagtgg gcagagcgct gccgtgccta attacttgat
     1981 ggccaacttc ctctggcgta aacgcgggcg catacaggaa gcggaacgag actatcgcaa
     2041 agccctggag gcggaccctc agtacttcga agctatggtg gcactggcag atctactcgt
     2101 gcaacacaaa aatgtctaca acgaatccaa cgacctctat ctaaaggcgc tcaagataag
     2161 ccccaagaat gccgaagcac atttgggtct ggccttgata aaagaacgtc tcttcaaaga
     2221 ctatggggcg gcagaaagcg aattgcgcgc agccctggcc atcaatccca ggtattcgca
     2281 ggccgctgat gccctcggtc aactgcaaga actctccctg ggtaaattct cggatgcccg
     2341 catctcctac aaagatgctg taaaaatgga tccgaacaac gctgatgcct ggttacacct
     2401 tggtctcatc aactggcaga aattccagga gagcaagacc gcccgcacgg agctggagaa
     2461 agcggccggt atggaaacgg gaaaatcggt ctatcgaacc gccctggcta ctcttttgat
     2521 tactgaattc aaagactaca aagaggcgga gaaactcctc acccagtcct gccagcaaaa
     2581 tttcgccgat tcggaagccc actatcgcct gggcatgctg ctcattgaaa aattcggcaa
     2641 acgcaaagca ggggaagccg aactcaagat cgcctacgaa caaaaccccc gggacaaaga
     2701 tatcaaagcc gcctatgagc gcttcgtacg ctaaaggcag catactgaaa ttgaaagaca
     2761 ggaattaaat taaccatgcc caggaacgaa ttcgatccta aatttgatga cgacgatgag
     2821 gatgatgatg acttcctcga tgaagaagac ctggaggaaa gagagagcga gaagcagggt
     2881 agaagctttg acctggaaga attgatcgca gcatgggaag acgattctcc cgataatctc
     2941 tattatttcg ataccaccac cggtgcggta aagatggtca accgaaacct ctttgacttg
     3001 agagaattga cggacgaaat cgaaaagaac aaagagcgct atctgtacct gccaaagccc
     3061 gatgccaaac aactgaaagg cgatttgaga gactttcaaa aatccatcga agatcagtca
     3121 ctcaagagag tcctggacat ggctttcgag agtccgcatc tgctatccag cttcaaggtg
     3181 atcttgaaga gccgggagtt ggtgggaacc cttgaacatt tcctgcgctc ccgctcctgc
     3241 ctgagaatca gacagtggat ggatgccaac gcccttaccg ataggtggca gatagaaggc
     3301 tgaccgctcg ggtagatccg gccggactga agctacggca accgaaaatg cagtccatta
     3361 gacaaatggt tctgcccgaa cattaacggg ctatcgcagc acgcacagac tgataaagat
     3421 ggcgttccag tccgggcaaa atatattcaa gtggtggtgg tgtagcgtcc ctgcggtaag
     3481 tttgaaccca tgccgcgttc cggcaaagcc ggtcctctca cgcatttaat agccgaccag
     3541 ggcaggtaga gcgtttcccc ttcccggcgc aagcacaaat atgtgctctg gtcgatcatt
     3601 gcgccctcgc cgcccacctg ctcgccgccc atcaagagaa cggtgactgg ggtgttcatg
     3661 ggaatggcca cgatcttgct cctaagctac taaccgacta catacattcc cacaaaaccg
     3721 gtaggaataa accttgttta tcgcgctcgt gtccggagaa gagcacccaa acggtttcag
     3781 agactcagac tagcagaagt ctgacttcca agtctagaag tgcaaacaag aggcagcggc
     3841 atgggggttg tttccagccg gagccagctc agaaataggc gtcatcagag ttcagcttac
     3901 caggactcag gctttctgcc gttggctcta gtttttactt cttcgatggc cttatgcagg
     3961 cttccgttgc cggtgttttc agccacatac ttggcctgct cggtggaaag tccaagcttg
     4021 cttgcatcat cgaatttatt ggatttgtcg ttgagactgt taagggcatt tatcagacgg
     4081 tctttggtag atatgagatt gccgaaaccc tcaaaattac cggtcaccat gcattgctgt
     4141 atggcagcat cgacgttggc aatggcacca atggccagac ctcggtaggc gtctgaagta
     4201 aaattaccag ccgagacggc ggcattcacc tgactggcta cgctgctacc gtgagatgag
     4261 gcactgttgg cagccgaagt ggatgccgcg taagattcgg ggctgctgct gtacacggtg
     4321 tagcgtccac cgctggggct gtcgtaataa acagtaccgg tgtcatgatt gacagtggca
     4381 ccgtcgaaat aactggagtg ctctcccaac tcatatttga gagtcttggg attctcatcc
     4441 tggctggtgt attccttgta tttgatatca gccggattgc tcatgtcaaa gacgcggtgt
     4501 ccctgttcgc cggcggtctg ctccactaca ccccgctccg gattgcgagg atcgatggta
     4561 gtggtttgaa tgggtttatc gtctgctccc ttgaattcaa caactcgctt atcatcgccg
     4621 tttgtggctg attgaacggt cttaccagca tccacgccgg tggtggccgt ctgcgaggcg
     4681 gtaacctgtt cagtagcacg gttcaagcga attcgtcccc gcccctccac atcgacggct
     4741 tccaggttac tggagtcggc attgatgcgc cagccgccaa aaccattggc attctgctgg
     4801 aggaattcgc gggtggaaat tgtccgacct ggaattggct caccatgagc atcgagtagc
     4861 tgcacctgat caccagagat acgggcaccg ccggaaccat cctttttaat aatggtgagt
     4921 tcgttggact gggacataac ggctatactt tcgccattat tgacaccttt accatttcgg
     4981 taatcctcca ctcctaaccg attgtctcgg ttgagaatgc cgggatcccc cggagcacga
     5041 ccttctctga tctgtcggaa atagtcacga gatacatcgc gagagtcttc agccagacga
     5101 gctgcataag caaaggtctg accgtaagtc ttctcaagtt cctggctgtt cttaacgcga
     5161 gtggtgatat cgtttgcctt gtcgtacagt tcgtaagtac cgtcctgcag tcttctaaca
     5221 gtgtcgccat tggtcttagt aatgatttga tctttgacac cgccgcgatc gtttgtgatg
     5281 tttactttgt caccatcctt caaactgatc tcgtaattgc gaccatcctt aactatggaa
     5341 acgccgtcag ccgttgtgtg ctcaaatttc ttgagattaa cgctaccgct ccaacaggtg
     5401 ttcgcatcgc caatcttgca ggaatcgacc gccccatctt tcttgacggt ttcaacgaca
     5461 acaccgttct cattcttgag agcgctaaca acatcactaa ctttcttgcc atcatcctta
     5521 agggaagtag caaattcatc gctgtagcta tcccagatag gagagaactt ttccttagtg
     5581 gaattataaa ggctctccag accgctacct ttatagatgg aataggtcac atcaccgacg
     5641 gactgaaaag tcgacttacc aaaatcccag atagctcctg taaatccagt ttcataccag
     5701 gacttgcttt caggtttagt agcatcagtc ggtttggaaa cgtccggagt ggcagccaat
     5761 ttttgctctc cactttcggc agtggttttg ggagctgcac tggtaccggc gtcggagaag
     5821 acatcaaatg aaaaattgcc aaaagagaat gcactactcc aatctccagc ctttgaatcg
     5881 ggactggatt cggacttgga ttctggcttg gattctggat tgggagttgc ggtctcctga
     5941 gcagcaagtg gctggctaga atctgtgttg ccttgcgctg tcaacgcggc cgctgccacc
     6001 accgtgtcag ccggcttaac tgccgtcgaa tctttccccc cttctacgct ggctccggtc
     6061 agcgatttag ctggtacgct gaaatcgag
//
LOCUS       DAOQNZ010000032         5959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5242_length_5959_cov_3.468327, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000032 DAOQNZ010000000
VERSION     DAOQNZ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5242_length_5959_cov_3.468327"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKW73_01030"
     CDS             <1..495
                     /locus_tag="PKW73_01030"
                     /inference="COORDINATES: protein motif:HMM:NF033832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HNA71892.1"
                     /translation="GEVRVELAVIGDILHGYEIKSEKDNLERLPRQALYYGRVFEKMT
                     LVAHERHVEEAVKIVPGNWGLIAVGKRDGKPYANEIWPARLSYELDPLALAQLLWRDE
                     ALELMEYFDLDHGLKSKPRKVLWQKLASALTLEQLKTFVCFKLRTRKGWKRPKRVKLV
                     EEFS"
     gene            complement(515..1195)
                     /locus_tag="PKW73_01035"
     CDS             complement(515..1195)
                     /locus_tag="PKW73_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA71893.1"
                     /translation="MPKILYVEDDKDLATMVKEWLVGENFTLELVHDGALGWEYLRQG
                     DYDLIILDWELPGMSGVEILKRYRNMSGMTPVLMLTGRGHVENKEQGFDAGADDYLTK
                     PFAMKELSARLRALMRRPAMMVSNAIKVGDLELDPTKHTVTKAGQPLHLLPKDFSLLE
                     FFMRNPDKVYSTEALMMRVWNADSESGTDALRTAIKRLRKALDGDTAESDSMIENIPR
                     IGYRLKSR"
     gene            1334..3142
                     /locus_tag="PKW73_01040"
     CDS             1334..3142
                     /locus_tag="PKW73_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA71894.1"
                     /translation="MKLSLTAKAIILISMPLIFEVGSAFLLLDLQKQAEEEAERANHS
                     RAVYDKINSISKEIYEVWNTASSVSRSSMLTTSFLNQSYKKTFGHLRKQYQELVNLSG
                     TKSKLAGAVGEAIKALDDAEKILDSTCEELRSGNYDEVFANHKVKEKNLKDLYLKMLS
                     QEFYLAEQEEEKLLKDSPMRLRQYRQRTINIFLIALAGNVVFSLILAVFLVGRVTNRL
                     GHLSYEVRKLGTGRPISAPLSGSDEIAGLSKVLSTMAVELDTLLRQQGAIVANARDTI
                     CAIDDKGCFTAMNPAGERLFGYYADEVRASRFVEYVYAADKEKALNWYQTLRSGASGE
                     EVELRIEREDGAVVTALWSGQWSEADRSLFCVIHDISERKELEQAKQELTAMLTHDLR
                     TPLTTIQACLEMLEAGRLGSLNARGVELVQVADRNGARMMNLINDLLDIEKIKSGTMS
                     LELAPVRIADMFQEVDLNLSQWMKESRIALKLQQTDLCVLADRDKICRVLFNLVSNAI
                     KFSPPGSRISLSTLREGHWVKISVTDQGPGIPADQLDLIFERFAQVSSAEHKGKGGSG
                     LGLAICRAIVNLHGGKIWATSSLGLGTTFYFTLPVA"
     gene            3313..4104
                     /locus_tag="PKW73_01045"
     CDS             3313..4104
                     /locus_tag="PKW73_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71895.1"
                     /translation="MVQVGVANLRRELEQLFPGKWLSAKESARVLKSGVESLDAVLSP
                     VLIRRHMSEWVGSASSGKTTVLRTICANWCATGLNIIYIDTFDRLLASDWALVGTENK
                     GRFWVLRSGDCADLDGQTRYGQHHKYDYVKNALWACEQLIRSHAFDVVILDLADRGVI
                     TSNINARLKRSLERSNASLVVLRDDDSTSSAFSGSVGRVDFNFIEPIKVEQGVSGPVA
                     IVPTIRSNVFKAGLSRSLEVSVAPYVPNRLFTHPPVPDRRTSKKR"
     gene            4196..5725
                     /locus_tag="PKW73_01050"
     CDS             4196..5725
                     /locus_tag="PKW73_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71896.1"
                     /translation="MLCSPEASREGIVPAMTFTEARAICSSLIWRAYDDKLYKNAQKK
                     LARELIALSPRVSCAEPGIFFIDAEGLGRLGGEGKLCRDLLKLASCHGFVDGRVGIAS
                     CAFAARIASAGTKKRWTMVPPGQDAHFMAPLSIDLLPLAAESRANLHGLGVKTIEQLL
                     ALPKPAFVGRFGDDVLRAYDLASGLDSFSPSLPEVDRVFQCAFDVGSSMDSLKETLFV
                     LKSMLDRLTAALRTQGFVAEELTARFLNDDELFEDRLIKLLRPSNNSRFLLDVLRLSL
                     ESKPLTREFTAVQLVVSRFSKEAFEQISVPNNEKEVRLDDSESITLLMQRLTTRLGED
                     VLVRPVASDHYTPEAAGFWQPVLNRASHACLLPVDRDYVDRYLGSYAPGKDSLMPGLV
                     LKRHVPAMPVFVQLTEESLGQGPLESSLPTAVTYKGQWYRISRITIPERISGMWWEVP
                     VRKSYYVALLERKELSLAARNIREFGRKGKLQTVLPSFMTVLLVFDHQERSWFVEGVF
                     D"
     gene            5727..>5959
                     /locus_tag="PKW73_01055"
     CDS             5727..>5959
                     /locus_tag="PKW73_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNA71897.1"
                     /translation="MAAIDYAELHCHSAFSFLDGASLPESLIVRARELGLYALALTDT
                     LDLGGMPRFSQAGRENGVDAIVGAELVLEDDSRL"
BASE COUNT         1345 a         1385 c         1625 g         1604 t
ORIGIN      
        1 ggtgaagttc gggtcgagtt ggctgtcatt ggcgatattt tgcatggcta tgagatcaaa
       61 agcgagaagg ataatttgga gcgcctgcca aggcaggctc tctattacgg tcgtgttttt
      121 gaaaagatga ctctggtagc ccatgagcgc catgtggaag aagccgtgaa gattgtgccc
      181 ggtaactggg gactcattgc cgtggggaag cgggacggca agccctatgc caatgaaatc
      241 tggccggcaa gactcagcta tgagcttgat cccctcgctc ttgctcagtt gctctggcgt
      301 gatgaagccc tggaattgat ggaatacttc gatctggatc acggcctcaa aagcaagcct
      361 cgcaaggtgc tctggcagaa actggcctcg gccttgactc tggaacagct taagaccttt
      421 gtgtgtttca aactacgcac tcgcaagggt tggaagcggc ccaagcgagt caaattggtg
      481 gaagaattca gttagccgct ctggataacg agttttatct ggatttgaga cggtaaccga
      541 tgcgagggat gttttcgatc atcgaatcac tctcggccgt atctccatcc agggctttgc
      601 gcaggcgctt gatagcggtt ctgagggcat cggtgccgga ttccgaatcg gcgttccaga
      661 ccctcatcat gagcgcttcg gtactgtata ccttgtccgg attgcgcatg aagaattcta
      721 gaagagagaa gtcttttggc agaagatgca ggggttgccc ggctttcgtg acggtgtgct
      781 tggtcggatc aagctccaga tcaccgactt tgatggcatt ggagaccatc atggccggtc
      841 tgcgcatcaa ggctctcagc cgtgcggaaa gctccttcat ggcaaagggc ttggtcagat
      901 aatcatcggc gccggcgtcg aaaccttgtt ccttgttttc cacatggccc cggccggtta
      961 gcatcaatac cggcgtcatg ccgctcatat tgcgatagcg cttgagaatt tccactcctg
     1021 acatgccggg aagctcccaa tcgagaataa tcagatcgta atcgccctgg cgtaagtact
     1081 cccagccgag ggcaccgtca tgcaccagct ccagggtaaa gttctcgccg acgagccatt
     1141 ctttcaccat agtggcgagg tctttgtcgt cttctacgta gagaattttt ggcatcgtat
     1201 tatcctgggc agggcaattc aagaatgact ttaaataatg acggtttcga gattcgacta
     1261 taaactatgg cagatgtcta tggaagattt tctggatgca aagtgtctct cattttctag
     1321 accggttagt taagtgaagc tttcactaac cgctaaagcg ataattctaa tatccatgcc
     1381 cctgattttt gaggtcggct ccgcttttct tttgcttgat ttgcagaaac aggcggaaga
     1441 ggaagccgag cgtgccaatc attcccgggc tgtatacgac aagataaatt ccatcagcaa
     1501 agagatatat gaggtatgga acacggcctc ttccgtcagc cgcagttcca tgcttacaac
     1561 ctcctttctc aatcaaagct ataagaagac tttcggtcat ttgcgcaaac agtaccagga
     1621 actggtcaat ctttccggca ccaagtccaa actggccggt gccgtcgggg aagccataaa
     1681 ggctcttgat gatgccgaga agatcttgga ttccacctgc gaggaactgc gctcgggtaa
     1741 ctacgacgaa gttttcgcca atcacaaggt gaaggaaaag aacctcaagg atctctattt
     1801 gaagatgctt tcgcaggagt tttatctggc tgagcaggag gaggagaaac tgctcaaaga
     1861 tagccccatg agattgcggc agtatcgcca gcgtaccatc aatatattcc ttattgccct
     1921 tgccggtaac gtagttttca gtctaatcct ggccgtcttc ctggtgggtc gggttaccaa
     1981 tcgcctcggg catctcagct atgaagtgcg taaactcggt accgggcggc ccataagtgc
     2041 gccgctttcc ggtagcgatg aaattgccgg cctgtccaaa gtgctttcca ccatggccgt
     2101 tgagctggat acccttttgc ggcagcaggg cgccattgtg gccaatgctc gtgataccat
     2161 ctgcgccatt gatgataaag gttgctttac ggccatgaat ccggccggcg agaggctctt
     2221 tggttattat gcagatgagg taagggcttc tcgctttgtc gaatacgtgt acgctgccga
     2281 caaggagaag gctctcaatt ggtatcagac cctgcgttcc ggtgccagtg gcgaagaggt
     2341 ggaactgagg attgagagag aagacggggc ggtggtgacg gctctctgga gcggccagtg
     2401 gtctgaggcc gatcgcagtc tcttttgtgt tattcacgat attagtgagc gtaaggaact
     2461 ggagcaggcc aagcaagagc tgacggccat gcttacccac gacttgcgca cgccgttaac
     2521 caccatacag gcctgcctgg agatgttgga agccggtcgg ctcggtagtt tgaatgcccg
     2581 aggcgttgag cttgtgcagg ttgccgatcg caatggtgcc cgcatgatga atctcatcaa
     2641 tgacctgctg gatattgaaa aaatcaagtc tggcactatg tctctcgaac tggctccggt
     2701 aagaattgct gatatgttcc aggaagtgga tctcaatttg agccagtgga tgaaggaaag
     2761 tcgtattgct ctcaaactac agcagactga tctttgtgtg cttgcggatc gagacaaaat
     2821 ttgccgggtg ctctttaatt tggtgtccaa tgccatcaaa ttttctccac ctggctcccg
     2881 catttctttg tctaccttgc gtgagggcca ctgggtcaaa atcagtgtta cggatcaggg
     2941 accgggcatt cccgccgatc aactggatct tatatttgaa cgctttgccc aggtcagttc
     3001 cgccgagcac aagggcaagg gtggctccgg cttaggcctt gccatttgcc gggccatagt
     3061 caatttgcat ggtggaaaaa tctgggccac cagttccctt ggtcttggca ctactttcta
     3121 cttcactctg ccggtagcct gattctccaa gtcgaaggcg tatatgtttt attcgcactt
     3181 taagtatgcg aacgaaagcc ttttgatatg aaaacgggaa agaaaaaggt tgactaccat
     3241 acggctgtat ggtttaattt cttcatcggg gaaatgcaat tgtgaattag aaaggaggct
     3301 atagcgctgg ctatggtcca agttggtgtc gctaatttaa gacgtgagct tgagcagcta
     3361 tttcctggga aatggctttc cgcaaaggaa agcgccaggg tgctcaagtc aggggtggag
     3421 tcgcttgatg cggttctttc tcctgttctt atccggcgtc acatgtctga atgggtaggg
     3481 tctgcttctt ctgggaaaac aacggtctta agaaccatct gtgctaattg gtgcgcaacc
     3541 ggcctgaata ttatttatat agatactttt gacaggttgc ttgcttccga ttgggccctg
     3601 gtgggcactg agaataaagg gcgcttctgg gttttaagaa gtggagattg tgctgatttg
     3661 gatggtcaga ccaggtacgg tcagcatcac aagtacgatt atgtgaagaa tgcgctatgg
     3721 gcctgtgagc agttgatccg ctctcacgct tttgatgtgg tcattcttga cctggcggac
     3781 agaggagtca tcacgagcaa tattaacgct agattgaaga ggtccctgga gcgatccaat
     3841 gcctctcttg ttgtgctcag ggatgatgac tccacttcct ctgctttctc aggttcggtc
     3901 ggtagagttg atttcaattt catcgagcct attaaggtag aacagggcgt cagtggtccg
     3961 gtggcaatcg tgcccactat caggagtaat gtctttaaag ccggtttgtc tagaagcctg
     4021 gaggtaagtg tggctcctta tgtaccgaat cgcttgttta cgcatccccc ggttcccgat
     4081 cgtcgtacat caaaaaagag atgagcttaa aggcaagcct tttgtcttgc ttgctgcaga
     4141 gcaatcataa aaaacaagtc taagtaaaaa tagcaatatt ggtagatgca aaattattct
     4201 ctgctctccg gaggcgagta gagagggcat tgtgccggct atgaccttta ctgaagcaag
     4261 ggcaatttgt tcgtcactta tctggagggc ttatgatgac aagttataca agaatgcgca
     4321 gaagaaattg gcgcgagaac ttatcgcact ctccccaaga gtcagttgcg ctgagcctgg
     4381 catcttcttc atcgatgctg agggacttgg tcgcctgggc ggcgagggca agctttgtcg
     4441 agatttactt aagttggcta gctgtcatgg ttttgttgac ggccgggtcg gcatcgctag
     4501 ttgcgctttt gcagcgcgta tagccagtgc cggcacgaag aaacgctgga caatggtccc
     4561 tcccggccag gacgcgcact ttatggcacc actttccatt gatctcttgc ctctggcggc
     4621 agagtcgaga gccaatctcc acgggctggg agtaaaaact atcgaacaac tgttggctct
     4681 accgaaaccg gctttcgtgg ggaggtttgg tgacgatgtg cttagagctt acgacctggc
     4741 ttccggtctg gattctttca gccccagttt gccggaggtt gatagggttt ttcagtgtgc
     4801 ctttgatgtt ggttcctcta tggattctct caaggagaca ctttttgttc tgaagagcat
     4861 gcttgaccgc ctcacggccg ctctgcgtac gcaaggtttt gttgccgagg agttgacggc
     4921 tcgcttcctt aatgatgatg agctatttga agatcgcctc atcaaacttt tacgcccgtc
     4981 caataacagc cgcttccttc ttgatgtctt aaggctttct ctggagtcga agccgcttac
     5041 ccgtgagttt accgctgttc aacttgtggt ttctcgtttt agcaaagaag cctttgaaca
     5101 gatttccgtt ccaaataatg aaaaggaggt gcgattagat gacagtgagt ccattacttt
     5161 gttgatgcaa cggctcacga ccaggctggg agaagatgtc cttgtgcgtc ctgtggcaag
     5221 tgatcattac actcccgaag cagccggttt ctggcagccg gtgcttaata gagccagtca
     5281 tgcttgctta ctgcctgttg atagagacta tgtagatcgt tatttgggct cttacgcccc
     5341 tggaaaggat agcttaatgc ccggtctggt acttaagcgt catgtaccgg ctatgccggt
     5401 ttttgtacaa ctaaccgaag aaagccttgg tcagggacct cttgagagtt ctttgcctac
     5461 ggctgttacc tataaaggac agtggtatcg cataagccgt atcaccatac cggagcgtat
     5521 ttccggtatg tggtgggagg tgcctgttcg taagtcctac tatgttgctc tattggagcg
     5581 aaaggagttg tctttagcgg ccagaaatat cagagagttc ggcaggaagg gaaaattgca
     5641 gacagtattg ccttctttta tgactgttct tttggtcttt gatcatcaag agcgcagctg
     5701 gtttgtggag ggtgtattcg actgatatgg ctgccattga ctatgccgag ttgcattgtc
     5761 attccgcctt ttccttcctt gacggcgcca gcttacctga gtcgcttata gtaagggccc
     5821 gggagctggg tctttatgcc ctggctctga ccgataccct tgatctgggc ggaatgcctc
     5881 gcttttccca agccggacgg gaaaacggtg ttgatgccat agtcggggct gaactggtgc
     5941 tggaagatga ttcccgcct
//
LOCUS       DAOQNZ010000033         5880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5359_length_5880_cov_3.507124, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000033 DAOQNZ010000000
VERSION     DAOQNZ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_5880_cov_3.507124"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKW73_01060"
     CDS             <1..548
                     /locus_tag="PKW73_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NIF family HAD-type phosphatase"
                     /protein_id="HNA71898.1"
                     /translation="THYRVFVDCDRTLVNTDLSSLISPRWFDKRPKFLTQGKLVLTEA
                     RPSAHAFLAALKSTYSVSILTLGHSKFQAKVLKELGLLSYVESIYGPDNWQELTKPDG
                     FVLIDDMSADSIGIAYKMRWLGRQRSLENLDAWDSMVNFHLIQCAPFMGGKTDQTPLT
                     EHLDDVHERMKAQMNGSLPAT"
     gene            complement(688..1716)
                     /gene="pip"
                     /locus_tag="PKW73_01065"
     CDS             complement(688..1716)
                     /gene="pip"
                     /locus_tag="PKW73_01065"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNA71899.1"
                     /translation="MSKTKLKDGKAGVAGKRRKLAVRPEGDYYPEIEPFCTGWLDVEG
                     GHQIYFEESGNPKGKPVVFLHGGPGGGTSPGYRRFFNPDVYRIILFDQRGCGKSKPFA
                     SLENNTTWHLVSDIEALREHFGIDKWMVFGGSWGSTLALAYAETHPERVTELVLRGIF
                     MGRKKELAWFYQEGASFIFPEFWKDYLAPIPMRERGNMIKAYYKRLTSKNEKVRLEAA
                     KAWSIWEGCASKLYVDKDLVARNAGDDFATAFARIECHYFINNCFFQSDSQLLDGVAF
                     LRNIPCTIVQGRYDMVCPATSAFELAQAWPEAEFVLVSDAGHSMSEPGIKKALLDACD
                     KYAGIKGR"
     gene            complement(1836..3893)
                     /locus_tag="PKW73_01070"
     CDS             complement(1836..3893)
                     /locus_tag="PKW73_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNA71900.1"
                     /translation="MNRVSRASTGRIGKRLYKFRVEKRWFLMPDGVKLAATLFVPQAA
                     RRKNGSPGETFPVLLEYLPYRKDDTFYIADYPCFSYLAQLGFLAVKVDIRGTGASEGV
                     CPEREYSDIEMQDGEEVIRQLAALPQANGNVGMFGVSWSGFNSLQMAMRRPPALKAIH
                     AVHASDDLFHDDVHYIDGNLHLDPYHLFINHELGLPPTPDYGIDEEYFSSRFDRRPWL
                     FTYLSKQLDGEFWSRKSLKEDYSRIDIPVYLMGGLLDGYRSATMRMYENLQVPVRCDI
                     GPWNHSCPDDGTPGPNYEWLDRMTEWFSRYLKPDLSGVEGTALDAPAKGARKTAVKPE
                     KELLVYVRHGHKPDKEIETVPGYWRQDVFPLKGAEALTLKLDGAGASSGKPLVYKPFG
                     GTAAGTWWGDTTGDMGGDDADSLVWDSSPQDKPLQIVGFPRVKLKVKASSPKAKWTVR
                     LEDVSPDGAVSLVTGALYNPAIREDRLKPAWPEADREYDLVTDLHFTSWTFRPGHKIR
                     LSVSNAQLIMTWPSPDAMTSTVDFAASSMTLPVVPIDEGKVDNLPQPKSKRQVRDCEG
                     LDFPGGKAESEVYQRRDYRTGDRTHTIKARSAYRIRGRKFFTENSNAWTANDEKPWAA
                     RYTGRASTVIVSRGRNLSLKTRILVRSDRENLYVFLSRTLTVNGKVVRQRRFRQTIAR
                     QFN"
     gene            4445..5134
                     /locus_tag="PKW73_01075"
     CDS             4445..5134
                     /locus_tag="PKW73_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71901.1"
                     /translation="MGKDKPICDSKEHVQQAQEKAEQESLLGQKFWSAIGMPIEGDKS
                     TVACTPKEQAEALQRPKLSAREEEQVTRNVQSLEAAIKTGDLTKVGELFNQMAPRADN
                     VVKPDEFHKRSGVKVTDETMHRLNKELDKYGINLDYRAMGVYQGDRSPSLIISRESPK
                     GNGSTEHVEVTLQGGSTKDFMRHSGTDRVTIAENGATSFDKVAGVGNRTGAEVAYQEI
                     MKPYLDAKKGK"
     gene            5570..>5880
                     /locus_tag="PKW73_01080"
     CDS             5570..>5880
                     /locus_tag="PKW73_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71902.1"
                     /translation="MPKTDTIQVTPELLALLTEIDEFKGAWRALGSIAPDRLRALRRV
                     ATIESIGSSTRIEGSSLSDREVERLLANLEIQKFESRDEQEVAGYAEVMETVFAAWQH
                     IP"
BASE COUNT         1458 a         1640 c         1599 g         1183 t
ORIGIN      
        1 ctacccacta tcgagtcttt gtcgattgcg acagaaccct ggtcaacaca gacctgagtt
       61 cacttatcag tccgcgctgg tttgacaagc gccccaaatt cttgacccag ggcaaattag
      121 tgctgaccga agccaggccc agcgctcacg cctttcttgc ggccctcaag agtacttaca
      181 gtgtctccat tttgactctc ggtcactcca aatttcaggc caaagtcctg aaggaactcg
      241 gtctgctcag ctacgtagaa agtatctatg gtccggacaa ctggcaggag ctcaccaaac
      301 cggacggctt tgtgctcatc gacgacatga gcgccgattc cataggcatt gcttacaaaa
      361 tgcgctggct gggtcgccag cgctctctgg aaaacctcga tgcctgggat agcatggtca
      421 acttccatct cattcagtgc gctcccttca tgggcggcaa gaccgatcag acgcccctga
      481 ccgagcattt agacgatgtg cacgagcgca tgaaagccca aatgaacggc agcctgccgg
      541 ctacctaaat gtgaaagcac aaatgaagag tgaccagaaa gctacctgaa catgaaagca
      601 caagagaaat cagctaacca catgcaatag tatatgttgc aaaaagagaa ggagagcagc
      661 ctgagccgcc ctccctctca acagttgcta ccgccccttg ataccggcgt acttgtcgca
      721 ggcatccagg agggccttct tgatgcccgg ctcgctcatg gagtgaccgg catccgagac
      781 cagaacaaac tctgcttccg gccaggcctg agccagttca aaagctgaag tggcagggca
      841 aaccatgtcg taacgaccct gcacgatggt gcagggaatg ttgcgcagga aagcaacgcc
      901 atccaaaagt tggctgtcgg actggaagaa acagttgttg atgaagtagt ggcactcgat
      961 gcgggcaaag gccgtggcaa aatcgtcccc ggcattgcgt gccaccaggt ctttatcgac
     1021 gtagagcttg gaagcgcagc cctcccagat actccaggct ttggcagctt ccaggcgcac
     1081 cttctcgttc ttgctggtca agcgcttgta gtaagccttg atcatattgc cccgctcccg
     1141 catgggaatg ggcgccagat agtccttcca gaactccgga aagatgaagc tggcaccttc
     1201 ctggtagaac caggccagtt ccttcttgcg acccatgaag atgccccgca agacaagctc
     1261 ggtgacccgc tccggatgag tctcggcata ggccagagcg agggtgctgc cccaggagcc
     1321 gccgaacacc atccacttgt cgatgccgaa atgctcacgc agagcctcga tatccgaaac
     1381 cagatgccag gtcgtgttgt tctccagcga agcaaagggc ttgcttttgc cgcagccgcg
     1441 ctggtcgaag agaatgatgc gatagacatc gggattgaag aagcgccggt aaccgggcga
     1501 tgtgccacca ccggggccac catgcaggaa gaccacgggc ttgcctttgg gattgcccga
     1561 ctcttcaaag taaatctgat gtccaccttc aacgtcgagc cagcctgtgc agaaaggctc
     1621 tatttcaggg taatagtcgc cctcggggcg aaccgcaagc ttgcgcctct ttccggcgac
     1681 gcctgctttt ccatctttca attttgtttt ggacatactt ggtccttctt tctgattttt
     1741 gttgaaagtc taaaggaaaa ctccggcaaa gatccagccc atgcaaacag cgaagcagtt
     1801 tgccctggcg cctcggctct gccggagaac ccttcttagt tgaactgacg agcgatggtc
     1861 tgccggaaac ggcgctgcct gacgactttg ccgttcacgg tcagggtacg cgacaggaat
     1921 acatagagat tttcccggtc ggacctgacc agaatcctgg tcttgaggct gagattgcga
     1981 ccccgggaaa cgatcaccgt gctagccctg ccggtataac gagcagccca gggcttctcg
     2041 tcgttggcgg tccaggcatt gctattttcg gtgaagaact tgcgccccct gatgcgatat
     2101 gccgaacgcg ccttgatcgt atgggtgcgg tcaccggtgc gatagtcgcg ccgctgatag
     2161 acctcacttt cggctttgcc gccgggaaag tcgagcccct cgcaatctcg cacctggcgc
     2221 ttgctcttcg gctggggcag gttgtctacc ttgccctcat cgataggcac caccggtaga
     2281 gtcatggacg aagccgcgaa gtccactgtg ctggtcatgg catccgggct tggccaggtc
     2341 atgatcagct gggcgttgct gacggaaaga cgaatcttgt gaccggggcg gaaggtccat
     2401 gaagtgaagt gcaggtcagt gaccagatcg tactcgcggt cggcctcggg ccatgccggc
     2461 ttgagacggt cttcccggat ggctgggtta tagagggcac cggtgacaag tgaaaccgca
     2521 ccatccggag aaacatcttc cagacgcaca gtccacttgg ccttcggcga agaagccttc
     2581 accttaagct tgacccgtgg aaagccgacg atctgcaaag gcttgtcctg aggggagctg
     2641 tcccagacca ggctgtcggc atcatcgccg cccatatccc ccgtggtgtc gccccaccag
     2701 gtgcccgccg ccgtaccacc gaagggcttg taaaccaggg gcttgccgct ggaagcaccg
     2761 gcgccgtcca gcttcaaagt aagagcctca gctcctttga gcggaaatac atcctgccgc
     2821 cagtagcccg gcacggtctc gatttccttg tcgggcttat gcccatggcg cacatacacg
     2881 agaagctcct tctcgggctt aacagcggtc tttcgtgcgc ctttggcagg ggcatccaag
     2941 gcggttcctt ccacaccgga gaggtccggc ttgaggtagc gactaaacca ctcagtcatg
     3001 cggtcgagcc actcgtagtt aggaccgggt gtgccgtcat cgggacaaga gtgattccag
     3061 ggcccgatat cgcaacgcac cggcacctgc aagttctcat acatgcgcat ggtggccgag
     3121 cggtagccgt ccagaagacc acccatcaag taaaccggaa tgtcgatgcg gctgtagtct
     3181 tccttgaggc tcttgcggct ccagaactca ccgtcgagct gcttgctcaa gtaggtgaac
     3241 agccagggtc gacggtcaaa tcgactgctg aaatactcct cgtcgatgcc atagtcggga
     3301 gtcggcggca gccccagctc gtgattgatg aaaaggtgat agggatcgag gtgcaggttg
     3361 ccgtcgatgt aatgcacgtc gtcgtggaag aggtcatcgg aggcatgcac ggcatggatg
     3421 gccttgaggg cgggcggtct gcgcatggcc atttgcagcg aattgaagcc gctccaggaa
     3481 acgccgaaca tgccgacatt gccgttggcc tgaggcaaag cggcaagctg ccgaatcacc
     3541 tcttcgccgt cctgcatttc gatatccgag tactctcgct ccgggcaaac gccctctgaa
     3601 gcgccggtgc cgcgaatatc aaccttgacg gccaggaagc ccaattgggc taaataactg
     3661 aaacaggggt agtcagcgat gtagaaagta tcgtccttac ggtaaggaag atactcaagg
     3721 agaacgggaa aggtctcgcc cgggctgccg ttcttacgtc tggcagcctg cggcacaaag
     3781 agggtagcag ccagcttgac gccgtccggc atcaaaaacc agcgcttttc caccctgaat
     3841 ttataaagac gcttaccgat tcttcctgtc gaagcacgcg atacacggtt cataacaggt
     3901 acctgaggtt ggattttctg ttggtagaca aactgcaaag cccgccccta taccagagca
     3961 gtcagttcca tcggtgctaa atgcagccgc aagaaactga aagtgatgga cttgagagca
     4021 ggattggcag tgtgggattg acccttggaa aagaaaagaa atagatgtac gcagaaacta
     4081 gggcacagaa cagaacatct caagattgcg cgctagttaa taagcttcaa attacctgtc
     4141 ataactatta agagctagcg cggctcgcca aaatcaccaa cagctagcga gacccgcaca
     4201 cgttgacatc catgtccaca cacgctcgtg cagcctggca aacccaaacg caacctctct
     4261 ctgccttctt ctcatcaatg acagagcaaa gcccgagagc aattccaaac atcaccggca
     4321 cagccctggt tgccaagggt tttcaaggct gcctgccggg gtatgttgta aaaggtgcat
     4381 accgcatatc cggttttccg gagaaagaac aacaccggca accacaagac ctggggtaac
     4441 tgttatgggc aaagacaagc caatctgcga ctcgaaagag catgttcagc aagcacaaga
     4501 gaaggcggag caagagagcc tcctcggtca aaagttctgg tctgccatcg gcatgcccat
     4561 cgaaggcgat aagtcaacgg tagcctgtac tcctaaagag caggcggaag ctctacagcg
     4621 gcccaagctt tcagccagag aagaagagca ggtgacacgc aatgtacagt cactggaagc
     4681 ggctatcaaa acaggcgatc taaccaaagt cggcgagctt ttcaatcaga tggctccccg
     4741 agccgacaat gtcgtcaaac ccgacgaatt tcacaaacgt tccggtgtca aagttaccga
     4801 tgaaacaatg caccgcctca acaaagagct cgacaaatac ggcatcaacc tcgactaccg
     4861 ggccatgggc gtctatcaag gcgaccgctc accttctttg atcatctctc gggaatcgcc
     4921 caaaggcaac ggttcaaccg aacatgttga agtaaccttg cagggcggct ccactaaaga
     4981 cttcatgaga cacagcggta cagaccgggt gaccatcgca gaaaatggcg ctaccagttt
     5041 tgacaaggta gccggcgtag gcaacagaac cggtgccgaa gtagcctatc aagaaatcat
     5101 gaaaccttat ctggatgcca aaaaagggaa gtgattctgc ttagttcgcg aactgagagc
     5161 atgaaaaggc agtagaaatc gacgcgagca caggcatact tatccgatag ggaagcctgg
     5221 gggcggtgat aattacgact ccatgaatgg tcagctggtc ggcgtttaag cccaagagcc
     5281 gcagcgatga aacatggcaa taaatatcgg acgttggcag acgattctga cagcgtattt
     5341 ggtggcatca gctttctcct ccttttcggc ctctaaaggc aaaagagtgc agaaaaccca
     5401 cagaattttt caagccaaca tacggacact actgaccgga ttgattgggc atcaaaagcc
     5461 tcaacaagac tcaactaagc ccgcttgcct cagcaatcgg gtgtaatcgg gtgacaatac
     5521 aggtggacaa tcgggtgtaa tcgggtgata atacgggtgg agtaattaaa tgcctaaaac
     5581 agataccatc caggtgacgc ctgaactact ggcattactg actgaaatcg acgagttcaa
     5641 aggtgcctgg cgcgcactag gcagcattgc gcccgatcgg ctcagagcgc tgcgccgggt
     5701 tgctaccatc gaaagcatcg gctcctctac ccgtatcgag ggcagtagcc tgagcgaccg
     5761 cgaggtagag cggttgcttg ccaacctgga aatccaaaaa ttcgagagcc gtgacgagca
     5821 ggaagtagct ggctatgccg aagtcatgga aaccgtcttt gccgcctggc aacacattcc
//
LOCUS       DAOQNZ010000034         5852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5403_length_5852_cov_3.776954, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000034 DAOQNZ010000000
VERSION     DAOQNZ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5403_length_5852_cov_3.776954"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKW73_01085"
     CDS             complement(<1..610)
                     /locus_tag="PKW73_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71903.1"
                     /translation="MNRQLQSDPKLEGGKTSRRHFLALSLLTMTGLAQPAALAGRMLE
                     DKSGRRRCLVVLTLSGGNDGLNTVVPYGMGRYYDLRPKLAVKGETVLPLDGMVGLNPS
                     MGELAALYKEGKLAVVQGVGYPEPNRSHFRSIEIWQTAQPDIIGDTGWLGRYLDLQDK
                     SDLASRMLAAVNIDPVLPKSLLARKITVPSVYDLGDFRFKVDS"
     gene            complement(662..1984)
                     /locus_tag="PKW73_01090"
     CDS             complement(662..1984)
                     /locus_tag="PKW73_01090"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNA71904.1"
                     /translation="MTEKLKGQQIRHLLRRAGFGFTPVEYLAYRDLEYEEAVERLLNF
                     EGEDNSALEALIESQNFDFTNGDDIKRWWLFRMLFTRRPLEEKLTLFWHGHFATSLKK
                     VRNPYAMYVQNRLFRKLCMGDFGNLLLAVSKDPAMIVWLDNQQNRKGKPNENFAREIM
                     ELFTLGIGHYSEADVREAARAFTGWQVKNGVFTFNKGAHDAGKKLFLAESGNFDGTQV
                     ISILSRHKQTGRFLAAKLLNFFLGANFDRALLKRVAGAYEVDRQIRPMLKTIFLSKAF
                     LSRENQFSLVKSPCQYVVGTLKHLQISTIDGDLPRLLSAMGQDLFAPPSVKGWDGGTA
                     WLSSDTMMARFNFANRIIGEKFDALKKQGSLKDLLAEKGVDDPRTCVAYFLQLLVEGD
                     VPPPVVKRLEDYLGKEPFLKGAEAKTLLDERLRGLLHLIMTLPSYQLV"
     gene            complement(1981..2481)
                     /locus_tag="PKW73_01095"
     CDS             complement(1981..2481)
                     /locus_tag="PKW73_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71905.1"
                     /translation="MTEQVLKKCSLLLCLAVGLTMANLTMPNLAQASEPLESRIEDSD
                     RVFLPGNFFTPAVASAPSVKRKPTPASVPSLEQLKLIESLTADQNKRLNELYRDYKSD
                     ISALNGELKGKPDAAAKASLQARINLRQNTLMEALKAIVKTEQMDELEKKKKGMTSDS
                     EVENLP"
     gene            complement(2592..2664)
                     /locus_tag="PKW73_01100"
     tRNA            complement(2592..2664)
                     /locus_tag="PKW73_01100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2629..2631),aa:Val,seq:gac)
     gene            complement(2724..2798)
                     /locus_tag="PKW73_01105"
     tRNA            complement(2724..2798)
                     /locus_tag="PKW73_01105"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2764..2766),aa:Cys,seq:gca)
     gene            3009..4019
                     /locus_tag="PKW73_01110"
     CDS             3009..4019
                     /locus_tag="PKW73_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA71906.1"
                     /translation="MHFSFLAQRVIQALVTLLTVSLVSFALMKSLPGDPLDILLGSPQ
                     KDIAPQELDTLRRELGLNQDPVKQYSLWLASWLAPVRDENLALGLSYRDGRPVKEVIM
                     ERLPATLALCGLALVLSFSLGIALGAILAYLRLRGERSPAVRSLSLVVSAALAGFYAM
                     PAFWLAFLLVAALALRPDLYPPPIFGISAPGKDVDLTIVAYLLPPALILSLRRLVKVA
                     LYVKELALQELSSPYVLSALAKGLSLFQVTCRHVLKNCLVPVVNLLGLSAPSLVGGSV
                     LIETIFCLPGLGRLSVEAAFGRNYPVLLSLSMLYGTVVVVASFAADCLSRSLDVRLKE
                     GN"
     gene            4021..4947
                     /locus_tag="PKW73_01115"
     CDS             4021..4947
                     /locus_tag="PKW73_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA71907.1"
                     /translation="MPSLRRLTPAQLTGLSILAVMVFLALLGLLAPPCLSSLSPASIN
                     VPPFQNPQHLFGTDGLGRDLLTMILKGAAGTLFVGLAAALSASIFGYCLGAFSALCGG
                     LVDSLLMRLVDALLSVPAIVLLLTISSLISSPEFTRTMLSVDGLKQLAQALSLTDHSL
                     GYLPILSVVAAIAATNWLESARVARAITQKLMTEEYMTAARSLGAGSLHLLRFHLGPA
                     LKAVAALETTLLTADAIIMESGLTYLGLGLGPATPSWGGMLKDAQSGLLCGNLHGAVL
                     PGLFLALTVMGINLLATGGSRQQQRARSKEQD"
     gene            5003..5509
                     /locus_tag="PKW73_01120"
     CDS             5003..5509
                     /locus_tag="PKW73_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71908.1"
                     /translation="MLKPFHCLSLLLVSGLMLSACQMGDVSYTANNMKHTSSTAAGTV
                     PDDLKSLIYPGATTSGAQTAQEQGKDAGDYSKYLQMVSSDAVEKVALWYEQVLKKEGW
                     NIEKNEQMSKTICIEGRMKEAEISITLADDAGKTNIIVSKSASSGAIPDEEAVENYKP
                     SKEVTPTD"
     gene            5559..>5852
                     /locus_tag="PKW73_01125"
     CDS             5559..>5852
                     /locus_tag="PKW73_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71909.1"
                     /translation="MTAEAALPNDEAGRPRFGSFIKAVSTEDKLEILAVVLDVVTSPP
                     DSLHRPCALGLTREELKREQPHIFALLKTEIRAVNIGFIQAGTVYQHLPPQPPQ"
BASE COUNT         1348 a         1670 c         1487 g         1347 t
ORIGIN      
        1 gggaatctac tttgaaacga aaatcaccca ggtcgtagac ggagggcacg gttatcttgc
       61 gggccaaaag cgacttgggc agcacaggat cgatgttaac agcggcaagc atgcgactgg
      121 caagatcgct cttatcttgc aagtcgaggt agcgacccag ccagccggta tcgccgatga
      181 tatcaggctg ggcggtttgc cagatttcta tggagcgaaa atgggagcgg ttcggctccg
      241 gatagccgac accctgcact accgccagct taccctcttt atagagagcg gcaagttctc
      301 ccatggaagg atttagcccc accatgccat ctaagggtaa gacggtttcg cctttaacgg
      361 ccagtttggg tcgcaagtcg tagtagcgcc ccataccgta aggaaccacg gtattgagcc
      421 catcattacc gccgctcaag gtgagcacca ccaggcaacg acgtcgaccg ctcttatcct
      481 ccagcatccg gccggcaaga gcagccggct gcgccagtcc ggtcatagtt agaagagata
      541 gagcaagaaa atggcgacgg ctggtcttgc cgccctcaag cttcgggtct gactgtaatt
      601 gacgattcaa cttcacttcc ttcaccttgg cctttcctac atcatattat cttagtttag
      661 atcagacgag ctgatagctc ggcagcgtca taatcagatg aagcagcccc cgcaaccttt
      721 catcaagaag cgttttggcc tcggcgcctt tgagaaaggg ctctttgccc aggtagtctt
      781 caaggcgctt caccacagga ggcggcacat ctccctccac gagaagttgc agaaagtaag
      841 ccacacaagt gcgcggatcg tcgacaccct tttctgccag caagtccttg aggctacctt
      901 gcttcttcaa ggcatcaaac ttttctccaa ttatgcgatt ggcaaagtta aagcgggcca
      961 tcatggtatc ggaagaaagc caggcggtgc cgccatccca acccttcacc gaaggtgggg
     1021 cgaagagatc ttgccccatg gccgaaagca gcctcggtag gtcaccgtct atagtgctaa
     1081 tctgcaagtg cttgagagtg ccgaccacat actgacaggg actcttgacc agcgaaaatt
     1141 gattttcacg agacaagaag gccttactga gaaagattgt tttgagcatg ggccgaattt
     1201 gccggtcgac ctcataggca ccggccactc tctttagaag cgccctgtcg aaattggcac
     1261 caaggaaaaa attcagcagt ttggcagcca ggaaacgccc tgtttgcttg tggcgagaga
     1321 gtatagagat tacctgggtg ccgtcgaagt taccgctttc ggcaaggaaa agctttttac
     1381 cggcgtcgtg agcaccttta ttgaaagtga agactccgtt tttgacctgc caaccggtga
     1441 aggcacgagc cgcttccctg acgtcagcct cgctgtaatg cccgattccg agagtaaaga
     1501 gctccatgat ttctcgggcg aaattctcgt tgggcttacc cttgcgattt tgctgattat
     1561 cgagccagac aatcatggcc gggtctttag aaacagccag caggagattg ccgaaatcac
     1621 ccatacagag cttgcgaaag agccggtttt gcacatacat ggcataaggg ttacgcactt
     1681 ttttaagaga agtggcgaaa tgcccgtgcc agaataatgt cagtttctct tccagagggc
     1741 gtcttgtaaa aagcattcga aacagccacc agcgcttaat atcatccccg ttggtgaaat
     1801 caaaattttg actttcaatg agagcctcca gtgccgaatt gtcttccccc tcaaaattga
     1861 gtaggcgttc caccgcctcc tcgtattcga gatcgcggta agcgaggtat tccactggcg
     1921 tgaagccgaa gccggctcgc cgcagcagat gtctgatttg ctgccctttc agcttctcag
     1981 tcacggcaaa ttctcaacct cactgtcact ggtcatgcct ttcttctttt tctccagctc
     2041 atccatctgt tccgttttca caatcgcctt cagtgcttcc atcaaagtgt tctgcctcag
     2101 gtttatgcgg gcttgcaggg aggctttggc tgcggcatcg ggcttacctt taagctcgcc
     2161 gttgagggcg gaaatatcgc ttttgtagtc tctataaagc tcattcagcc gcttgttctg
     2221 gtcggccgtc aggctttcaa tcagcttcag ttgctcgaga ctgggcacag aagccggggt
     2281 cggcttacgt ttcaccgacg gagcagaggc tacggcagga gtaaagaaat tgccgggcaa
     2341 aaagactcta tcgctatctt caatcctgct ctccaatggc tcagaggcct gagccagatt
     2401 gggcatagtc agattggcca tagtcagccc gaccgccaga cagagcagta gggaacactt
     2461 tttgagaact tgctctgtca taaaccctcc agggaagata acgctttatc taccctaaaa
     2521 gttcaatgcc gaataaaaaa tggcagcggc aaaaaaaagc ccccccactt ggggagggct
     2581 cttctgaaat ttggacggta ctggactcga accagtgacc cccactatgt caaagtggtg
     2641 cgctaccaac tgcgccaacc gtccgtctta cttattctag ctttctaaaa ggtctctggc
     2701 gcaagcctta aagaaataaa aattggaggc ggcacccaga ttcgaactgg gggataaagg
     2761 ttttgcagac ctctgcctta ccacttggct atgccgccgc acttatggca tcgaaatgcc
     2821 cgagcacttg cactcacaag cgcactgatg taagcgcact tatgtaagca tacatgctat
     2881 ttccctatct ctacatttgt caattgcaac caccttattg aaactgaaca aatagacaga
     2941 accagcacac agttggcgtt atcataggca aaattgtgtt tagtgcttct taatagcctg
     3001 aggctcagtt gcatttcagc tttctagcgc aaagagtaat acaggcactc gtcacccttt
     3061 tgacggtttc cctggtcagc tttgccctca tgaaaagcct gccgggagac cccctcgaca
     3121 ttctgctcgg ttcgccgcaa aaggacattg ccccccagga gttggacacc ctgcgccggg
     3181 aactggggct gaatcaggac ccggtcaagc agtacagcct ctggctggcc tcctggttgg
     3241 cgccggtccg ggacgagaat ctagcccttg gtctctctta ccgggacggc cgacctgtga
     3301 aagaagtaat catggagcgc ttaccggcca ccctcgctct ttgcgggctg gccctcgtcc
     3361 tttctttcag cctgggcatc gccctgggag cgatcctggc ctacctccgc ctcagaggcg
     3421 aaaggtcccc ggcggtccga agcctcagcc tggtcgtttc agccgctctg gccggcttct
     3481 atgccatgcc cgccttctgg ttggccttcc tgctggtagc tgccctcgcc ctcagaccgg
     3541 atctctatcc ccctccgatc ttcggtatca gtgcgcccgg caaagatgta gacctgacca
     3601 tcgttgccta tctcctgccc ccggctctca tactcagctt aaggcgcctt gtcaaagtag
     3661 cactttatgt aaaggaactg gctctgcaag aactttccag tccctacgtg ctctcagccc
     3721 ttgccaaggg actttcgctc tttcaggtca cctgccgtca tgttctgaaa aactgccttg
     3781 tgccggtggt aaatttgctc gggctctcgg ccccatccct ggtgggtggc tcagttttga
     3841 ttgaaaccat cttctgcctg cccggactgg gaagactttc tgtggaagca gccttcggcc
     3901 gaaactatcc ggtgctcctc tctctcagca tgctctacgg caccgtcgta gtggtggcat
     3961 cctttgccgc cgactgcctg agccgcagcc tggacgttcg tttgaaggag ggaaattagc
     4021 ttgcctagcc tgagaagact cacccccgcc caacttaccg gtctaagcat ccttgccgtg
     4081 atggtctttc tggcgctcct gggtttactt gcgcccccct gcctgagcag cctttcgccc
     4141 gcaagcatca atgtgccgcc ctttcaaaac ccacagcacc tgttcggcac ggacggtctg
     4201 ggacgggatc ttctcaccat gatcctgaag ggagcggctg gtacactctt tgtcggtctg
     4261 gccgccgccc tgagcgcctc tatatttggt tactgcctgg gggctttcag cgccctttgc
     4321 ggcggtctgg tagacagcct tctcatgcgc ctcgtagacg ctctcctctc ggtgccggcc
     4381 attgttttac ttctgaccat aagctcgctc atctccagcc ccgagttcac ccgcaccatg
     4441 ctatctgtgg acggactgaa acaactggcg caggcactgt ccttgactga ccattccctg
     4501 ggttatttac ctatcttgag cgtggtggcg gccatcgccg ccaccaactg gctggaatcg
     4561 gccagggtgg ccagggccat cacccagaaa ctcatgacgg aagagtacat gacggccgcc
     4621 cggtcactgg gtgccggttc cctgcacctg ttgagatttc acctgggacc agccttgaaa
     4681 gccgtggccg ctcttgagac cacccttctc accgccgacg ccatcatcat ggaatccggt
     4741 cttacctatc tgggcctcgg tcttggaccg gccacaccca gttggggggg aatgttaaaa
     4801 gatgcccaga gcggactctt gtgcggcaac ctgcatgggg cggtgctgcc gggactcttc
     4861 ctggccctga ccgtaatggg catcaattta ctggcaaccg gcggcagcag gcaacagcaa
     4921 agagcaagga gcaaagagca agactaaagc tgcggtaaaa tacctcaaga ttctcaagtg
     4981 aaagctaaat ccaggaaata aaatgctcaa accttttcac tgtttaagtt tgctactggt
     5041 ctcagggcta atgttgtcgg cctgccagat gggcgacgtc agctacactg ccaacaatat
     5101 gaagcatacg tcatcaacag cggccggaac cgtgcccgat gatctgaaat cgctgattta
     5161 tcccggtgcc actacaagtg gtgcacaaac cgcgcaagaa caggggaaag atgccggtga
     5221 ctacagcaaa tacttgcaaa tggtctcttc cgatgccgta gaaaaagtag ccctctggta
     5281 tgaacaagtc ttgaaaaaag aaggctggaa cattgaaaag aacgaacaga tgtctaagac
     5341 catttgtatc gaaggacgca tgaaggaagc ggaaatcagc atcaccctgg ccgacgatgc
     5401 cggcaaaacc aatattatcg tcagcaaaag cgccagttcc ggtgccatac cagacgaaga
     5461 ggcagtggaa aattacaaac ccagcaaaga ggtcactccc actgactaga ctcaagcaaa
     5521 aagccctggc cgaggtcctc accgccagca ttgtcggttt gacggccgag gcggccctcc
     5581 ccaatgatga agccggtcgt cccaggttcg gcagtttcat caaggccgtg tccacggaag
     5641 acaaactgga gattctggca gtggtactgg atgtggtgac aagccctccc gattcattgc
     5701 accgcccgtg cgcacttggt ttaaccaggg aagaattgaa gcgggagcag cctcatatct
     5761 tcgccctttt gaaaacagag attagagcgg taaacatagg cttcattcag gccggcacgg
     5821 tctaccagca tctaccgcct caaccgcccc ag
//
LOCUS       DAOQNZ010000035         5785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5501_length_5785_cov_3.087435, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000035 DAOQNZ010000000
VERSION     DAOQNZ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5501_length_5785_cov_3.087435"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..567)
                     /locus_tag="PKW73_01130"
     CDS             complement(196..567)
                     /locus_tag="PKW73_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71910.1"
                     /translation="MLVKNANYFWIPAYLVGIVTAAASLNLLPTGPGVVLASILAVAM
                     LTPPSLWHSACDSIELHVGLYGIVLCAAILFAGLYIPDSLGPMAFPLGKGWEPVSWYQ
                     ILFVTLQQVFYFGIHAFTSDD"
     gene            complement(647..1381)
                     /locus_tag="PKW73_01135"
     CDS             complement(647..1381)
                     /locus_tag="PKW73_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71911.1"
                     /translation="MSGLTVRLFFNWRNIMCRRMFVLLNLVAMLFLLTACAYEDDSQT
                     RINVDAVEKALKEIQVDDQQGWMDAFEKRVNEIYEGDGIVSVDSRMEGDMLKITGYVE
                     KNSEDGLQPADEEIFSIKQTSPANEKSTEAVITSNTANGSPVEHHTSFLNTMFQYWLI
                     HKLISPSHAYHTPGASLAGLSYFRDNYRRSSSWTEQRRMNTAYNKRRPAIQLGGTTSH
                     SGIVLKKAASTGSKLSRSTYRRRSKR"
     gene            complement(1497..1862)
                     /locus_tag="PKW73_01140"
     CDS             complement(1497..1862)
                     /locus_tag="PKW73_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71912.1"
                     /translation="MTGKGYSALVGAMLAVFGVIVGVMLVISLFSMVLPWYLSVPVAG
                     GAMWFVLTKTKFGLRDKVWYTTGGLSFLVTTLVTMPFSGPFMAGVWGLVGLIVGMYVS
                     YHVLKAVVRNSPDPARWTD"
     gene            complement(1983..2660)
                     /locus_tag="PKW73_01145"
     CDS             complement(1983..2660)
                     /locus_tag="PKW73_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71913.1"
                     /translation="MNKNKWVATALAWCFSALMVYGCVMIGVSIADDLTEGPTGVSFY
                     GVLLALVLGVSVYGLCLTVFDNLTLDYQDYGQASFWLGVIAAVCVLMVPFYTVLQGGH
                     ERVLAYQKGAPELQSYLQVNFARLDRCAEPVDSGILLESDLEACLDKSPLYPFEVRLL
                     KEAQEQFKLLGHVLRIDESIVPISIATGNGGIMVMPMESDTKVYGISLSDLDGYQSRV
                     AALLRRW"
     gene            complement(2784..3539)
                     /locus_tag="PKW73_01150"
     CDS             complement(2784..3539)
                     /locus_tag="PKW73_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71914.1"
                     /translation="MYEKEFSLKVVVDGLELPVHQHEGKFYVAAPVGKKYELKVRVPW
                     ASFNRYAAVLSVDGLSVMNGKRATLADSAYVIDRAHGGVLTVPGFRLNDEEVAHFVFG
                     APEESYAALMDKPENIGVIAAVIYAEKCPVRETGWGDDLLGGHLTRGGGMTMGGGGLL
                     GGGGGMKGGNFPTRSGGHDVGTGFGDRTEHKVQQVQFERGEVVARIVIEYASEESLKA
                     AGIIKTSPLGDVSPFAAPEKKGEGCALPPGWKG"
     gene            complement(3776..4642)
                     /locus_tag="PKW73_01155"
     CDS             complement(3776..4642)
                     /locus_tag="PKW73_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNA71915.1"
                     /translation="MSTRFLINAGIWAAFTLILMMMFGQPFYMVDNGYAGVETCWGKV
                     QKGAISAGPGVKLPWCEITQVQVQTRTLKDVKINAPTIDLQELNGEATVQYAILSSAA
                     DEAYGVLGAEADIEENIILPGLHESTKVVTAQYTGTKLFYARDVAQLDMSKALQEWVD
                     NSAKARKLNRLIVIQSVVLTKANFTNTFETALQNKAIRDQVLQRAGAKRDTQLEDAKA
                     KAAIVTRNADADAANIRLNAEAEAQGMKNQAEQLLKNPQLLCYQARSKWKGTLSEIGH
                     GATPFDELCRGK"
     gene            complement(4655..5620)
                     /locus_tag="PKW73_01160"
     CDS             complement(4655..5620)
                     /locus_tag="PKW73_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNA71916.1"
                     /translation="MNDFNEKEYQVNDFRSLYANHRIGMLALSFLLIFGILFHTFTAA
                     VPTGSVGFKNFMGKLDVEGYHEPGLVLRIPFVQWLQYYPTRTYAETHTTQPRDDTNQV
                     LTAKWNVQLFVDPTYAGVLLKEIGSFDALRQKVLDPEISSSAQAETPTYSPEDLVKKR
                     PQLVANIKARLQKNLDNRLKEYKLPEGTVQVIEIACLDFFFSPAFNDSIEKKVEQDQL
                     KQTAEINRKIAEIQADADGQEKELLGKAEAYRTATVTSAQAYAIDIKGSASRLSTLLA
                     PYIQIERWNGEPHRVDLTGKSDVLIPGAAVPAPVVSEPAQSANQN"
BASE COUNT         1446 a         1566 c         1412 g         1361 t
ORIGIN      
        1 agggatgcga tcagcccgat ggcagagaat accgtcaggg caagcataat tttgacggcg
       61 ttgtcggtgc cgaaacgagt ttcaagtgca ctgtagaatt tttggtacat ggatttctcc
      121 ggtgtttttt taattgaaag ggttgtttga attgcactac tgagagtgcg actaccgcga
      181 tttcaggcag cgccctcaat cgtcggaggt aaaggcatga atgccgaagt aaaagacctg
      241 ttgcaaggta acaaagagaa tttgatacca ggagacaggt tcccagcctt tgcccaatgg
      301 aaatgccatc ggacccaaac tgtctggtat gtacagtccg gcaaataaaa tagccgcaca
      361 gagcacgatt ccgtatagac ccacgtggag ttcgatagaa tcacaggctg aatgccagag
      421 tgaaggcggc gtcagcatgg ctaccgcaag tatggaagcc aagacaactc caggtccggt
      481 gggcaaaagg ttgaggctgg ctgccgctgt aacgataccg accagatagg caggtatcca
      541 aaaataatta gcatttttga ctaacataaa ttgaactcct ttagaaacaa agacagaacc
      601 aaccgcacgg acaagccggt caatgggcag tacggttaga gcccaactag cgcttacttc
      661 ttctgcggta agttgagcga ctgagcttag accctgtgct tgccgccttc ttcagaacaa
      721 ttccagagtg gctcgtggtg cctcccagct gaatggcagg gcgacgctta ttgtaagcag
      781 tgttcatacg ccgttgctca gtccaggacg agcttctgcg gtaattatct cgaaagtagg
      841 ataaaccggc aagactcgct ccaggggtgt ggtaggcatg actggggcta atcagcttgt
      901 ggatcaacca gtattgaaac atcgtattta aaaaagaggt atgatgctcc accggactgc
      961 cgttagccgt attgcttgtt atcactgctt cagtcgattt ttcattagca ggagaagttt
     1021 gctttatgga gaagatttcc tcgtctgccg gttgcaagcc atcctcactg ttcttttcca
     1081 cgtagcctgt gattttgagc atgtcgccct ccatccggct atccacggaa acaattccat
     1141 cgccttcgta tatttcattg actcgctttt caaaggcatc catccaccct tgctggtcat
     1201 cgacctgtat ttccttaagt gccttctcca cagcatcaac attgatgcgc gtttgactat
     1261 catcctcata tgcacaagcg gtcaacaaga acagcatggc taccaggttt agcaaaacaa
     1321 acattcttct acacatgata tttctccaat tgaaaaatag tcgaacggtt aaaccgctca
     1381 taaaaccctt cctgaagggg acgctcctgc aagagaagcg tccgcacaga taggaatttg
     1441 cgacagccat ttaggcttat gccgcagcct tagctactac cggacaaagc ccgatatcag
     1501 tcagtccagc gagccggatc aggggaatta cgcaccacgg ctttcaagac gtggtaactg
     1561 acgtacatgc ccacaatgag cccgaccaag ccccagacac cggccatgaa cggcccactg
     1621 aacggcatgg tgacaagggt agtgacgaga aaggacaggc cccccgtggt gtaccatacc
     1681 ttgtctcgca aaccgaattt ggttttcgtc agcacgaacc acatggcgcc gccggctacc
     1741 ggaaccgaaa gataccaggg cagaaccatg ctgaacaagc taatcactaa catcacgccg
     1801 acgatcacac caaagaccgc caacatagct ccaaccaaag cactataacc tttaccagtc
     1861 atttcattct ccaaaattga gtttaaggaa gacgcttccg ggaacgggaa gcgcctggtt
     1921 tgatgggctt taagcccgat taaacaggct caattgagag cccggattta ttgccgaaag
     1981 acttaccagc gcctcaagag tgcagccact cttgactgat agccatctag gtcggaaagg
     2041 cttatgccgt acaccttggt atcggactcc ataggcatca ccataatgcc cccattacct
     2101 gtagcgatcg agatcggaac gatagattca tcgatgcgca gtacatgccc cagcagcttg
     2161 aactgctcct gcgcctcctt cagaagcctt acttcaaaag gataaagcgg acttttatcg
     2221 aggcaagctt ccaggtccga ctcaagcaga atgccagagt ctactggctc ggcacagcga
     2281 tcgaggcggg caaagttcac ttgcaagtaa ctttgcaatt ccggcgcccc cttctgatag
     2341 gccagaaccc gctcgtgacc gccctgcagc accgtataga aaggcaccat cagcacacaa
     2401 acagcggcga taacaccgag ccagaagcta gcttgtccat agtcctgata gtccagagtg
     2461 agattatcga agacggtaag acacaaacca tagacgctta ctcccagaac caaagccaat
     2521 aatacgccgt aaaatgagac gccggtcggc ccctcggtca ggtcatctgc aatggacacg
     2581 cctatcatta cacagccata gaccatgagt gcggaaaagc accatgccag agccgttgct
     2641 acccatttgt ttttattcat agtagagcct cataaagttg aacaaagtca gacgcctcca
     2701 aagcggtggc gtctgactat ttcagaagtc agcgaagcaa tgagcagaag ctgagcctca
     2761 acctaccact ccagccaagt tttctagccc ttccagcccg gaggcagagc acagccttcg
     2821 ccctttttct ccggagcagc aaagggtgat acatcgccca gaggggaagt tttgatgatg
     2881 cccgcggctt taagcgactc ttcgctggca tactcgatga cgatacgcgc caccacttca
     2941 ccgcgctcaa attgcacctg ctgcactttg tgctcggtgc ggtcgccaaa gcccgtacct
     3001 acgtcgtggc cgccgctacg ggtcggaaaa tttccgcctt tcatgccgcc accgcctccg
     3061 agcagtccgc cgccgcccat ggtcattcca ccgcccctgg tcaaatgccc acccagtaag
     3121 tcgtcacccc aaccggtctc tctcaccggg cacttttcgg cgtagataac ggcagcgatg
     3181 acaccgatgt tttcgggctt gtccatgaga gcggcataag actcttccgg tgcaccgaag
     3241 acaaagtggg ccacttcttc atcgttcaga cgaaagccgg gcaccgtcaa gacgccgccg
     3301 tgcgcacgat cgatgacgta agcactgtct gccaatgtgg cgcgtttgcc gttcatgacg
     3361 ctcagaccat cgacagaaag caccgccgca tagcgattga aggaagccca aggcactcgc
     3421 actttcagct cgtacttctt gcccaccggt gccgccacgt agaatttgcc ttcatgttgg
     3481 tgaacgggca attccagacc atcgacgacc actttcaagg agaattcttt ttcgtacatg
     3541 tttgttcctg ctagtttcaa atgtttgagt tgcgagcgaa attcgttgta atttcgctcg
     3601 cggttggttt tgagcattcc ctgcggcaag tcattcgttc atgcgtattc gtatgcgaat
     3661 gcgaattcga acgttcatgt tcatgttccc gttctcattc acgttctcga acacccgaac
     3721 taaagcccta actccaggcc tctgacttgc cttaactcag accacaggtg ctggtctact
     3781 tgccgcggca caactcatcg aagggagtcg caccatggcc aatctcggaa agagtgcctt
     3841 tccacttgct gcgagcttga tagcaaagca gttgtgggtt cttcaacaac tgctccgcct
     3901 ggtttttcat accctgggct tccgcttcgg cattgaggcg aatgttggca gcgtcggcat
     3961 cggcgttgcg agtgacgatg gccgcttttg ctttagcgtc ctccaactgc gtgtcgcgct
     4021 tggcaccggc acgttgcaaa acctgatctc gaatggcttt gttctgcagt gcggtctcaa
     4081 aggtattggt gaagttggcc ttggtcaaga caacactttg aatgacaatc agccgattca
     4141 gcttacgagc cttggccgag ttgtctaccc attcttgcag tgccttgctc atatccagct
     4201 gggccacgtc gcgagcgtag aacaacttgg tacctgtgta ctgagcggtc actaccttgg
     4261 tgctttcgtg caaaccaggc agaatgatgt tttcttcaat atccgcttca gccccgagaa
     4321 caccgtaggc ctcatcggca gcactgctca atatggcgta ttgcacagtg gcttcaccat
     4381 tgagctcttg caagtcgatg gtgggagcat tgattttcac atccttgagg gtacgggttt
     4441 gcacctgcac ctgcgttatt tcacaccagg gaagtttaac tccgggaccg gctgaaatcg
     4501 cccctttttg caccttgccc cagcatgttt ccacaccggc ataaccgtta tcaaccatgt
     4561 agaaaggctg cccgaacatc atcatgagga tgagagtaaa agccgcccaa ataccggcgt
     4621 ttatcaaaaa acgtgtactc attatcaata ctccttagtt ttggttggca gattgggcag
     4681 gttcggatac caccggcgcc ggaacagcag caccagggat aagcacatcg ctcttgccgg
     4741 tcaggtcaac ccggtgcggt tcaccgttcc agcgctcaat ctgtatgtag ggagccagaa
     4801 gtgtgctgag cctggaagca gagcctttga tatcaattgc ataagcctga gccgaagtaa
     4861 ccgtcgcggt gcgataggct tccgccttgc ctaataattc cttctcctga ccatcagcgt
     4921 ctgcttgaat ttccgcaatc ttgcggttaa tttcagcggt ctgcttgagc tggtcctgtt
     4981 ctactttctt ttcgatactg tcattgaaag caggcgagaa gaagaaatcg agacaggcaa
     5041 tctcaatcac ctgcacagta ccttcgggca gcttgtactc tttcaggcga ttatccaggt
     5101 tcttctgcaa gcgagccttg atgttggcca caagctgagg acgcttttta acaaggtctt
     5161 caggcgaata ggtaggagtt tcggcctgag cagaagagga aatctcaggg tccagcacct
     5221 tttgccgcaa ggcatcaaaa ctgccaatct ccttaagtag aacaccggca taggtgggat
     5281 ccacaaagag ctgtacattc cacttggcgg tgagcacctg gtttgtatca tcccggggct
     5341 gagtggtatg agtctcggca taggtgcgag tgggatagta ttgcaaccac tgcacgaagg
     5401 ggattctcag aaccagaccc ggttcatggt agccttctac gtcgagctta cccatgaagt
     5461 tcttgaaacc gacagaaccc gtgggcacgg cggcagtaaa agtgtggaag agaataccga
     5521 aaatcaggag gaagctcagg gcgagcatcc caattcgatg gttggcgtac aagctgcgaa
     5581 aatcgttcac ctggtactct ttttcattga agtcattcat atttgacctt ctaaagtctg
     5641 agttaaaaaa aaacgagaac gacctatcct gtcgctctcg ctcgaaattg acgaatgacg
     5701 tcctgtcatt cgttttgccg gttacccgaa ttagccgctg cgactcttaa gccacaagac
     5761 gtaagtaacc cggtgctacc tgcca
//
LOCUS       DAOQNZ010000036         5781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5507_length_5781_cov_4.311212, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000036 DAOQNZ010000000
VERSION     DAOQNZ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5507_length_5781_cov_4.311212"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..650
                     /locus_tag="PKW73_01165"
     CDS             156..650
                     /locus_tag="PKW73_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71917.1"
                     /translation="MNYGDNSMKIAGIDPGVTGAVAIIEDGQLPKIFDTPAMVLHKPG
                     GKSRTEYDLQGMAKILESERPDRVVIEEIQPMQLGSIAAFSIGGGYRAWLMACAIFQI
                     PVEKVSPQKWKKLVGLPKGADKEASRLKALEHFPEKYESIKLKKDHNKAEALLIALYG
                     LLQK"
     gene            647..3169
                     /locus_tag="PKW73_01170"
     CDS             647..3169
                     /locus_tag="PKW73_01170"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNA71918.1"
                     /translation="MIDIEGVLLPALFERLDTAFPEFGWVRDSRGWRATNEQTCKSTT
                     GASQRRVVCHRPVGFYAHGAGNTTWFQYLTGERSPRGEAFTDALKELCARAGVTYTAR
                     LITEEEREEIERRQKLGSVLEDIWQVVRNELQVEELNARKKYLVSRDLSAEPDGVDVG
                     YCHRTEAVCKALYDRGYTPEELSLAGFTSRHKETGEWQANTLWDTRIVGKYLSKRGHI
                     VGLWGRSTEPESKRKYVNTTGFDWNEHPVSKATGGSRERLVLVEGVFEPEIFKTTIPD
                     IASIGGAGNKCTKEFWEDLADSGTKTAVLLLDSDEPGLKGREAALASYRRADRRPDLY
                     VAQLPEGIKDADECRRAHGTNEVQESIRNAKHVCRFVVESTLADCPEGAEWTDEARND
                     AARALIKEAAHWDVLERSQYFLPAIQHICPSFDSEGLAELVAEQERKQQEKQALVKLA
                     SLAQGLTRDVKEAGLASTVQNAIRELQSLVPSAHQSVEPIRSVAEALEPHKQWLNEYR
                     GKGPMIGLPQVTLPEVDRSMMGLRGLIILPGPPNTGKSALGHQFGMDVVTNNDNACFV
                     YVALEMRKEDHINRMWSRLAGMDYKTFRTGSSIASEPMAAFTNDELTRLHSAEVQLNV
                     LGSRILILDKSNCPHPTAEGLKQIITDFKVSTGCERCFVLVDYLQRWPVPAALERELV
                     TDLQKDDWRMEQMEKLCTSPDDPVVVISAQAKHEATGSFGKLARVKGSGSGSYSPDAA
                     LFIRPADDEEVYKQRMKYDIDPSTPPSKAEKLKEKLEEMGVYKELAKNGWQWCWLELV
                     KGRDGMERFELPITFHFRQSSFTEGWAMPPVWVNAQREINAA"
     gene            3647..4000
                     /locus_tag="PKW73_01175"
     CDS             3647..4000
                     /locus_tag="PKW73_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA71919.1"
                     /translation="MSFGEEVRKIRLEKRIGLREFCRRINKDPSYWSKVEREQTKVSW
                     DGNLERALQDVLELPYNELKRLYFYARFQTLSYQARTFDDLRLMLPVFIHTVNGKKPS
                     KKQLIELAELIKHNK"
     gene            4007..4363
                     /locus_tag="PKW73_01180"
     CDS             4007..4363
                     /locus_tag="PKW73_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71920.1"
                     /translation="MRKKLNSARRHQISLLIVLLAHARLQIQYFLKSLPQEQELEDVR
                     FFMKSLFAHLREIELALGSARQKWDAHDEREIDAAKEAKREREDMEYIRRDTKATLRA
                     LKEWQWRKPRQTVKYE"
     gene            4397..4747
                     /locus_tag="PKW73_01185"
     CDS             4397..4747
                     /locus_tag="PKW73_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71921.1"
                     /translation="MKRDDYEELMGFSGTHKQDGTLLVHFDDFDRLRDAAKVLIVQNA
                     ILNRAVKILSQAASDYCDRLEIENFGEDCEYSVAIAQAATHYGLANPGDSAFDLKGLT
                     AKKLRGALGIIVDE"
     gene            4786..5013
                     /locus_tag="PKW73_01190"
     CDS             4786..5013
                     /locus_tag="PKW73_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71922.1"
                     /translation="MISFTDQQLDKLPEAEKIMYEAYCALEAILGWIDSGKNKSAQSF
                     AEAEHQSRIAHLLLHQALDRPCYQNLLGATE"
     gene            5033..5230
                     /locus_tag="PKW73_01195"
     CDS             5033..5230
                     /locus_tag="PKW73_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71923.1"
                     /translation="MDNTNRIEVRRYFFRVPSAVYVEVLATNDIAAKLKLDGTQRKER
                     GEAVMYRDYERAELIGSLPTE"
BASE COUNT         1704 a         1248 c         1599 g         1230 t
ORIGIN      
        1 caagccgagt cggaagcata tgcttactct ccctgagaac acggtggcat tgttgaagta
       61 tgtcgctgcc tgggaaaagg aaaccatgtc caagttagtg atacggcaga ttaacactct
      121 cttgaaagac tggaaagaag cgaatccggc gaagtatgaa ctatggagac aacagtatga
      181 agattgcagg gattgaccct ggtgtaaccg gggcggtggc tattattgag gacgggcaac
      241 tgcccaaaat atttgacacc cctgctatgg tcttgcacaa gccaggtggc aagtctagga
      301 cagagtacga cttgcagggc atggctaaga tactcgaaag tgagcgccct gatagggtgg
      361 tcattgaaga gatccaacct atgcaactgg ggtcgattgc tgcgttttca atcggcgggg
      421 gttatcgcgc ctggctcatg gcttgtgcaa tatttcaaat tccggtggag aaagtttcac
      481 ctcagaagtg gaagaaactg gtcggcttgc cgaaaggtgc ggacaaagaa gcttcgcgct
      541 tgaaggcgct tgaacacttc ccggaaaagt atgagtcaat caagttgaag aaagaccaca
      601 ataaggcaga agcattgctt atcgccttgt acgggttgct tcaaaaatga ttgacatcga
      661 aggcgtcctt ttaccggctt tgtttgagcg gcttgacact gcttttccag agttcggctg
      721 ggttagagat tcaagaggat ggagggcgac taatgaacaa acttgtaagt caactacagg
      781 cgcaagtcaa cggcgtgtcg tttgtcatag acccgtgggc ttctacgccc atggagcggg
      841 aaacacaaca tggttccaat accttacagg tgagcggagc cctagaggcg aggctttcac
      901 agatgcgctc aaagaactct gtgcaagagc cggcgttacc tacacagcta gactaataac
      961 tgaggaagaa agggaagaga tagagcgcag gcagaagctg ggctctgtcc tggaggacat
     1021 atggcaagta gttcgtaatg agctacaagt ggaggaactt aatgcaagaa agaaatattt
     1081 ggtctctcgc gaccttagtg ctgagcctga cggtgtggac gttggctact gccatcgtac
     1141 ggaggcggta tgtaaggcgc tttacgaccg gggctatacc ccagaagagc tatcgctcgc
     1201 aggctttacg agtaggcaca aagagacggg ggagtggcag gcgaacactc tctgggacac
     1261 gcgcattgtt ggaaaatatc tatcgaagag agggcatata gttggactct ggggaaggag
     1321 cactgaaccg gaaagcaagc gcaagtatgt caacaccacc ggcttcgact ggaatgaaca
     1381 tcctgtcagc aaagccaccg gcggaagtag agaaaggctt gtccttgtcg aaggagtctt
     1441 tgagcctgaa atcttcaaga ctaccattcc cgatatcgct tcaatcggcg gagcgggaaa
     1501 taaatgcact aaggaatttt gggaagacct tgccgactcc ggcactaaaa ccgccgtcct
     1561 actcctggac agtgacgaac cagggcttaa gggacgggaa gccgcactgg caagttaccg
     1621 ccgtgccgat aggagaccgg acttatacgt tgcacaacta ccagagggta tcaaggatgc
     1681 agatgaatgc aggagagcgc acgggacgaa tgaggtacag gaaagcatac gtaatgccaa
     1741 acacgtttgc cgctttgtgg tggaatctac tctggctgat tgcccggaag gggcagaatg
     1801 gactgatgag gcaaggaatg atgcagctag agctcttatc aaggaagcgg ctcactggga
     1861 tgttctcgaa agatcccagt actttcttcc tgctattcag cacatctgtc catccttcga
     1921 ctccgaggga ctagcggaac ttgtagcgga gcaagagcgg aagcagcaag agaaacaagc
     1981 gctagtaaag ctcgcaagcc tagcccaggg gctaacgaga gacgttaagg aagctgggct
     2041 ggcatcgact gtgcagaatg ccattagaga actgcagtcg ttggtgccaa gtgcgcacca
     2101 gtccgtagag cctatccggt cagtagcgga agcgctagag ccgcataagc aatggctgaa
     2161 tgaataccgg ggcaaaggtc cgatgatagg gctcccccag gtgacattac cggaagtcga
     2221 taggtcgatg atggggctcc ggggtttgat tattctccct ggtcctccca acaccggcaa
     2281 gagtgcatta ggtcatcagt ttggaatgga tgtcgtgaca aataacgaca atgcttgctt
     2341 tgtgtatgtc gccctggaga tgcggaaaga ggaccatatc aaccgcatgt ggtctcggct
     2401 tgccggaatg gattataaaa ccttccgcac tggctctagt attgcttccg agcctatggc
     2461 ggctttcact aatgatgagc tcaccaggtt acattctgcg gaggttcagc taaatgtcct
     2521 tggctcccgt atcctcatcc tggacaaatc gaattgcccg cacccaacgg cggaaggact
     2581 gaagcaaata atcacagact tcaaggtatc aacaggctgt gaaagatgtt ttgtgctggt
     2641 cgattacttg cagcgctggc cggttccggc ggctctggaa agagagctag tcacggactt
     2701 acagaaggac gactggagaa tggagcagat ggagaagcta tgcacttcac cagatgaccc
     2761 ggtagtggtg attagcgctc aggcaaagca cgaagccact ggaagttttg gcaagctcgc
     2821 cagagttaag ggctccggct ctggtagcta ttcgcctgat gctgccttgt tcatccgacc
     2881 ggctgatgat gaggaagtct ataagcagcg catgaaatac gacatcgacc caagcactcc
     2941 accaagtaaa gcagagaagt taaaagagaa actggaagag atgggggttt acaaagagtt
     3001 agcaaagaat ggctggcaat ggtgctggtt ggaattggtc aaaggcaggg acggtatgga
     3061 gcggtttgag ttgcctataa ccttccactt ccgccagagt agttttaccg aaggctgggc
     3121 aatgccgcca gtatgggtta acgcacagag ggaaataaat gcagcgtaat ggagcaatgt
     3181 tgacgccggc attagccagg caatcacggc aatcaatgcc gtcggatgtg gattggtggc
     3241 gagcattttg gaggtgggcc gacaagaagc tagaagccaa acaaatgaca caaacacagt
     3301 tggaagcgaa cgaatcaatc atggagaaag aatatgcgaa gccttttagt cgttagtttg
     3361 ttggttggtt gtgctctgcc ggcaagagtt ggtagtaact gatttaacat tactgggagg
     3421 ttttatgccg gtctatcaag tgagaacaaa gtttagcgcc atcagaacat gggaggtcga
     3481 agctaaaagc cagaaggaag cagaagagaa agggcacgcg cggactattc aatactggct
     3541 atccaaagca gttttcggca gacgggggag cggcgacaaa atacgcacct tatcagtgca
     3601 tgaagtaccc gcaagtaatc aggaatcact aggagataac taaagcatga gtttcggtga
     3661 agaagtgagg aagatacgcc tagaaaaacg tattggtctg cgcgagtttt gcaggcgtat
     3721 caacaaagac cctagctact ggtcgaaggt cgagcgagag caaacgaaag ttagctggga
     3781 tggcaacctg gaacgggctt tgcaggacgt gttagagctt ccatataacg aactgaaaag
     3841 actatatttc tatgctcgtt tccagacctt gagctaccaa gccagaactt ttgacgacct
     3901 gcggctaatg cttcccgtgt ttattcatac ggtaaacggc aagaaaccaa gcaagaagca
     3961 gctaatagag cttgcagaac taattaagca caacaaatag agagttatgc ggaagaaatt
     4021 aaacagcgcc aggcgccacc aaatcagcct cttaatcgtg cttctcgctc atgccaggct
     4081 tcagattcaa tattttctga aaagccttcc tcaagagcaa gagctagaag atgtgcgctt
     4141 cttcatgaaa tcactcttcg ctcacttgcg agaaatcgaa ttggcactag gaagcgccag
     4201 gcagaaatgg gatgctcacg acgaaaggga gattgacgcc gcgaaagaag ctaaacgcga
     4261 aagagaggac atggaataca ttaggagaga tacgaaagcc acactgagag cgcttaaaga
     4321 atggcagtgg agaaagccaa ggcaaacagt caaatatgag taagcacaaa ctgattagag
     4381 attatcggga gattacatga aacgagatga ttatgaagag cttatgggct tttccggtac
     4441 ccacaagcaa gatggaacat tattggttca ctttgacgac ttcgaccgcc tgcgagatgc
     4501 agctaaggtg ctcattgtcc aaaacgctat cctaaaccgg gctgttaaga ttttgagcca
     4561 agcggcttct gactactgcg acaggttgga gattgagaat tttggagaag attgtgagta
     4621 ttccgtagct atcgcacaag ctgccacaca ttatgggctt gccaatccag gcgatagcgc
     4681 ctttgacctt aagggactga ccgccaagaa gctgcgtggc gctcttggaa ttattgtcga
     4741 tgagtagata agcacaaact gataacaagt tacacggaga ttatcatgat tagctttaca
     4801 gaccaacaat tagacaagct gcccgaagcc gagaagataa tgtatgaggc gtattgcgcg
     4861 ctggaagcca ttctaggttg gattgatagc ggtaaaaaca aatccgctca atcttttgct
     4921 gaagccgagc atcaaagcag aatagcgcac ctactgttac atcaggcatt agatagaccg
     4981 tgctatcaaa atttattggg cgcgactgag tgacaataag ccgggagaaa ctatggacaa
     5041 cacaaacaga atagaagtta ggcgatattt tttccgtgtg ccttcagccg tgtacgtaga
     5101 ggtactggct accaatgaca ttgcagccaa gttgaagctg gatggcacac aacgaaaaga
     5161 gcgaggggaa gccgtcatgt accgagacta tgaaagagca gagcttattg gttccttgcc
     5221 aactgagtga caataagccg ggagaaaccg aagtgaaacg cacagaatac gcaagcaatg
     5281 gcggcagcgg tagaaaagtt cacaagcgag aacaaagcaa aggagacgac atgacaggaa
     5341 acaaatacgt agcctatatc tctttgtata ggaccaaggc tttctctttc caggccgaca
     5401 atgatgaaca ggcattatct ataggtaagt cgtgcatgga cacattgctc aggaatcacg
     5461 gcttcactct tatgctaccc agcagggtga gtagtaacaa aatcagtgat ggtgaagact
     5521 gttgtagcga gttcctaagc gtcttcaagg aacaggattc gcgattgcgg ccaattcctg
     5581 ggggatctaa cgtgcagccc aacggaaaag cacaaactga taacaagtaa cttggaggta
     5641 acaacaaaaa tgaaaacgca accctggcaa ttagcgcaac agatagcagg tgagtttaga
     5701 atgctggaaa gcctgttaac gcaacaagct cgcctagaaa ttgacatcgt aaacgagcga
     5761 gcccgcatta agaagatggt c
//
LOCUS       DAOQNZ010000037         5766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5534_length_5766_cov_3.795482, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000037 DAOQNZ010000000
VERSION     DAOQNZ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5534_length_5766_cov_3.795482"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKW73_01200"
     CDS             complement(<1..375)
                     /locus_tag="PKW73_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71924.1"
                     /translation="MDANEAREGRAKLLAGAAAKFAEADDAPLRLPEWVKRSVLNTQE
                     NRETRLILKEENLNTICESGRCPNKGECWAKGTATFMLMGALCTRTCRFCSVNKGTPS
                     PLDPEEPQRIATAAQKMGLRHVV"
     gene            512..1345
                     /gene="tpiA"
                     /locus_tag="PKW73_01205"
     CDS             512..1345
                     /gene="tpiA"
                     /locus_tag="PKW73_01205"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNA71925.1"
                     /translation="MSSKVRKTIIAGNWKMHKTVAEAQALAEAVLQGVAKELAGEALP
                     DIILCPTYVSLPAVLNVVNAPDGKTANKQNCFVGAQNMEHRDSGAFTGEIAPGMLTHL
                     GVTHVILGHSERRQFFGETNQGVNLKTKAALAHKLVPIVCVGETLDEREGGLTDQVIR
                     RQVAAAVEDLSAEEVAKIVVAYEPVWAIGTGKNCESPEANRVCKMIRSTINELYGTSV
                     ADGIPVLYGGSVKGSTIDEQMAQSDIDGALVGGASLKAEEFLPIIKGGAKRLKLMATK
                     A"
     gene            1434..1521
                     /locus_tag="PKW73_01210"
     tRNA            1434..1521
                     /locus_tag="PKW73_01210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1468..1470,aa:Ser,seq:gga)
     gene            1581..2672
                     /gene="prfA"
                     /locus_tag="PKW73_01215"
     CDS             1581..2672
                     /gene="prfA"
                     /locus_tag="PKW73_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNA71926.1"
                     /translation="MEHFVEKLKDIERTFNELEQKLSDPAIIGDQSTFMRLARTRHQL
                     ERTVSAYHRWLAAEKRISEAQELLRTESEKEMRELAEMELEEAREEHQNLTGELKILL
                     LPKDPNDEKNIMLEIRAGTGGDEASLFAGDLLRMYLKYADRVGYTPEIASCSEGEVGG
                     YKEVIVSFKGDAAYSKFKFESGVHRVQRVPATEASGRVHTSTATVAVMPEAEDIDVQL
                     NDNDLDISTMRSGGAGGQNVNKVETAVRIVHRPSGLMVACSEERSQLQNKERAKQILR
                     NKLYQMQLEEQQKAIYDERKSQVGSGDRSEKIRTYNYKDNRVTDHRLNLNFSLQQVLE
                     GDLDKLIEACILQHQQRLLEAEMNTVAGI"
     assembly_gap    2838..2937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2938..3797
                     /gene="prmC"
                     /locus_tag="PKW73_01220"
     CDS             <2938..3797
                     /gene="prmC"
                     /locus_tag="PKW73_01220"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNA71927.1"
                     /translation="AIGISTNEAAAEARLALATILKVKEGSLVIEGDRLLGEAASVLE
                     LVLADRLKRRPLQYALGEADFFGMTFAVGEGVLIPRPETELIVEAVLARLQETGPRLI
                     IEVGGGSGCIFVSLLKHFAGARALVFEVSPEAMRYCLQNAAQHQVEGRSRVLHQDFFA
                     PETGAILREAMAIEPVSGKPWPIAPIFVSNPPYIPAQTMASLEPEVQDYEPHIALCGM
                     DEDGLGFYRGFAGALAEFCPPGLDFFLEVGEGQADAVAEIFARHGFGDIDIKEDLAGI
                     KRIVSGRLN"
     gene            3903..4778
                     /gene="hslO"
                     /locus_tag="PKW73_01225"
     CDS             3903..4778
                     /gene="hslO"
                     /locus_tag="PKW73_01225"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNA71928.1"
                     /translation="MKDGLLKAISADGTIRAVIATTTELVSCARRKHDLSYTATAALG
                     RALTAGVLLAPMLARDGQVALKFHGNGPLGRVVVDASPRGTVRGYVEHPQVELPLNSL
                     GNFDVGGAIGSSGYLHVTVDSGFGAPHTSTVELASGEVGEDVNRYLVTSDQTGSLVVV
                     GTHLTSKGVEAAGGLLVQLLPDHTEETICKIENNITTFGTFTFLMRRGMSLENILQRI
                     LNGFEVRPLTDVKPISFYCKCSQERFVEALKVLPANDLLSMAEEDGQAEGRCQFCNEL
                     YLVKKEGLLDLAKGQ"
     gene            complement(4841..5044)
                     /gene="rpmB"
                     /locus_tag="PKW73_01230"
     CDS             complement(4841..5044)
                     /gene="rpmB"
                     /locus_tag="PKW73_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNA71929.1"
                     /translation="MAKRCDVCGKGPQTGVQYTFLRSHFNPHGKRRWLPNLQPVKAMI
                     GKTVKRLKVCTSCIKAGKVQKAI"
BASE COUNT         1300 a         1398 c         1592 g         1376 t
ORIGIN      
        1 gaccacatgg cgcagcccca tcttttgggc ggcagtggcg atgcgctggg gttcttctgg
       61 gtcgaggggg gaaggcgtac ctttatttac tgaacaaaag cgacaagtac gtgtgcaaag
      121 ggcgcccatg agcatgaagg tggcagtgcc tttggcccag cattcgcctt tgttggggca
      181 acgaccgctc tcgcagatgg tattgaggtt ctcttccttg agaatcaatc ttgtctcgcg
      241 attctcctgc gtattcaaga ccgagcgttt tacccattcc ggtaggcgca agggggcgtc
      301 gtccgcctct gcaaatttgg cagcggcgcc ggctagaagt tttgctctgc cttctcttgc
      361 ttcgtttgcg tccatttccc agcggttcct tctttatttc attgcgattt tgcccgaact
      421 acagagattc tagtttatag agatgtagaa ttagacgact gttgcaaata tctatgcctc
      481 attagcagtt actagtgtgg aggagttgtc cgtgagttca aaagtacgca aaaccattat
      541 tgccggcaat tggaaaatgc ataaaaccgt tgccgaagcc caggctctgg ctgaagccgt
      601 gcttcagggg gtggccaaag aactggccgg ggaagctctg ccggacatca tcttgtgccc
      661 tacttatgtc agcttgccgg cggtcttaaa cgtggttaat gcgcctgacg gcaaaaccgc
      721 taataagcag aattgctttg tcggtgctca aaatatggag caccgcgaca gcggtgcttt
      781 caccggcgaa attgcccccg gcatgctcac ccacctgggt gtgacccatg tcatcctggg
      841 acattccgaa cgccgccagt tttttggtga gaccaatcag ggcgtcaatt tgaagaccaa
      901 ggcggccctg gcgcacaagc ttgtgccgat tgtttgcgtt ggtgaaaccc ttgacgagag
      961 agaaggtggt cttaccgatc aggtgattcg tcgtcaggtg gcagccgctg ttgaagattt
     1021 gagcgcagaa gaagtggcta aaattgtcgt agcctacgag cctgtatggg ctatcggcac
     1081 cggcaagaac tgcgaatcgc cggaagccaa ccgggtttgc aagatgatcc gcagcaccat
     1141 taatgaactt tacggcacct ccgtggctga cggtattccc gttctgtatg gcggttccgt
     1201 caaaggcagc accatcgacg agcaaatggc tcaatccgat atcgacggtg ccctggtggg
     1261 cggagccagc ttgaaggccg aggaattttt gccgattatt aaaggtggag ccaagcgcct
     1321 caagctaatg gctaccaaag cctgaaggca taaagttttt tttctttgct acaaggaccc
     1381 gtcacaggac gggtcctcaa gtagcgttgc ttgttataat cgacaaacca cttggagagg
     1441 tgtcggagtg gtctaacgtg cagtcctgga aagactgtct gcgtcaaaag cgcagcgagg
     1501 gttcgaatcc ctccctctcc gaatttttca tcaggctcac ttacttttga gcactacatc
     1561 agaggtgcca ggctcttcat atggagcatt ttgtcgaaaa gctcaaagat attgagcgga
     1621 cctttaacga gctggaacaa aaactctctg atcccgcgat tatcggcgat caatctacgt
     1681 tcatgcgcct ggcccgcacc aggcaccagt tggagcgcac tgtcagcgcc tatcaccgct
     1741 ggttggctgc cgaaaaacgt atctcggaag cgcaggaact cttgcgtacc gagagtgaga
     1801 aggagatgcg tgaactggcc gagatggaac tggaggaagc tcgcgaagag caccagaacc
     1861 tcaccggtga actgaaaatt ttgcttttgc ccaaagaccc taacgacgaa aagaacatca
     1921 tgcttgagat tcgtgccggt acaggcggcg atgaagcatc gttgtttgcc ggtgatctgt
     1981 tgcgcatgta tttgaagtac gcagataggg tcggttacac cccggaaata gccagttgca
     2041 gtgaaggtga agtaggcggt tacaaggaag taatcgtcag ctttaaaggt gacgccgcct
     2101 acagcaaatt caagttcgaa tccggtgtgc atcgcgtgca gcgtgtgccg gctaccgagg
     2161 cttccggacg agttcatacc tccactgcca cagtggctgt catgcctgaa gccgaggaca
     2221 tcgatgttca gttgaacgac aatgacctgg atatttccac catgcgttca ggtggagccg
     2281 gcggccagaa cgtcaacaaa gtagagactg ccgtgcgtat cgtgcacaga ccctcaggtt
     2341 tgatggtggc ttgctcggaa gagcgttctc agttgcaaaa caaagagcgg gctaagcaga
     2401 tcttgcgtaa caagctctat cagatgcagt tggaagagca gcagaaagcc atttatgacg
     2461 agcggaagtc gcaggtcggc tccggtgacc gttccgagaa gattcgtacc tacaattaca
     2521 aagataatcg tgtcaccgat catcgtctca atctgaattt ttcccttcag caagtactgg
     2581 agggagatct ggataagctt attgaggcct gtatcctgca acatcaacag cgcctgcttg
     2641 aagcggaaat gaacacagtt gccgggatct aaatctgatc gggcgccggg ggaagagccg
     2701 ccggccaacc atggtggatc gcccctcttt tcgaaagcac agaccgtcag ctcggcgcgc
     2761 ctggcctttg cccgggccct gaaggccatt ggtatttcca caaatgaggc cgctgccgaa
     2821 gcccgtcttg cccttgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagg
     2941 ccattggtat ttccacaaat gaggccgctg ccgaagcccg tcttgccctt gccaccattt
     3001 tgaaggtgaa ggagggtagt ctggttatcg agggtgatcg tttgctgggg gaagcggcct
     3061 cggtactgga actggtactg gccgatcggc tcaaacggcg gcccttgcag tatgccctcg
     3121 gtgaggctga tttctttgga atgacctttg ccgtgggcga aggggttttg attcccaggc
     3181 cggagacgga actgattgta gaggccgtcc tggctagatt gcaagaaacc ggaccgcgcc
     3241 tgatcataga ggtgggtggc ggctccggct gcatttttgt cagcctgcta aagcactttg
     3301 ccggggccag ggcccttgtt ttcgaggttt cgccagaggc catgcggtac tgcttgcaaa
     3361 acgccgcaca gcaccaggta gagggcagga gccgggtctt gcaccaggac ttcttcgcac
     3421 ctgagaccgg tgcgattctg cgggaggcca tggctattga gccggtttca ggcaagccct
     3481 ggcccatagc gcccattttc gtctccaacc caccttatat accggcacaa acaatggcta
     3541 gtcttgagcc cgaggttcag gactacgaac cccacattgc tctgtgcggc atggatgagg
     3601 acgggcttgg cttttaccgg ggcttcgccg gcgctcttgc tgaattctgc ccaccggggc
     3661 tggatttctt cctggaggta ggtgaaggtc aggcggatgc ggtggcggag atctttgccc
     3721 gacatggctt tggggatatc gatataaaag aggatctggc cgggatcaag cgcatagtga
     3781 gtggccgtct caattaaatt accctttaaa aaatgtaata tgctgctatg atggggaaat
     3841 agagcctttt ggggatttcc tatcggcact gtgagaatcg ggcttaagca aagaatcaag
     3901 caatgaaaga cggacttctc aaagctattt cagccgatgg caccataagg gcagtaatag
     3961 ccaccacaac tgagctggtc agttgtgcca ggcgcaagca cgatctttcc tacactgcta
     4021 cggctgcctt agggcgagct cttacagccg gcgttttgct tgccccgatg cttgcccggg
     4081 atgggcaggt ggctttgaag tttcatggca acggacccct gggccgggta gtggtggatg
     4141 catcgccgcg cggcacagtt agaggctatg tagagcatcc ccaggtcgag ttaccactta
     4201 actccctggg gaattttgat gttggggggg ccataggatc ttccggttat ctccatgtca
     4261 ccgttgattc cggtttcggt gctccccata cttccacggt ggagctggcc tccggagaag
     4321 tgggcgagga tgtaaaccgc tatctggtaa cttccgacca gaccggctcc cttgtggtcg
     4381 tgggtacgca cttgacgagt aagggggtag aggcggccgg tggtcttctg gtgcagctcc
     4441 tgccggacca tacggaagag accatctgca agattgagaa caacatcacc acgttcggca
     4501 cttttacttt tctgatgcgg cgcggcatga gtcttgaaaa cattctgcag cgcattctga
     4561 acggatttga agtgcgcccc ctcaccgatg tgaagcccat cagcttctac tgcaagtgct
     4621 cccaggagcg ctttgtggag gctttgaagg tgttgccggc caatgatttg ctttccatgg
     4681 ccgaagagga cggtcaggcg gaggggcgtt gccagttttg taatgagctt tatttagtaa
     4741 agaaagaggg acttctcgat ctggccaaag gccaataacc gaaaaatccc ttgagagagc
     4801 tttcaatttc aatactgttc tagtttttcg gcttagatgc ttagattgcc ttttgcactt
     4861 taccggcttt gatgcaagaa gtgcacacct tgaggcgctt cactgtcttg ccgatcatcg
     4921 ctttgaccgg ctgcaaattg ggcaaccagc gtcttttacc gtgcgggtta aagtgcgacc
     4981 ggaggaaggt gtattgcaca ccagtctggg gtcctttgcc gcatacgtca caacgcttag
     5041 ccattttgaa taatcccgtc tatttaccca ggcgcctgtt tattcggcgc taactaactg
     5101 acccgcttca gggtcgttaa cgacctctca cgagacttct gcgtgctcat ctaccgacgg
     5161 atatcggaag ctgcaagcac aagcaaagtg cgaattgtac cacgttggat atatgccgga
     5221 gcggtttttt gcgttctttg caaagtttgt aatagttcta agcgaaagcg attttttgcc
     5281 tcattcttgc ccgtcactgg gtataaatcg tgatagtcta aacttcagga accgtcgaaa
     5341 agttcatgct gtttggcggt tgtaaggagt ccttgtcaga gttactagag agcctcatac
     5401 cagtgggcct cacgggagcc tgttagcgga aggtaagaaa tctggtttac aagattgttc
     5461 ttgacagacc ttttaaggta ccttttagag cggtcttatg gagcacttct attacactcc
     5521 aaatcgtggt atcaattctt ggcggttcga aaatatccgc ttgatatcag caggctaaag
     5581 aagctcgaaa atattcggca aacaagcagt tggcacaaat cagttgtatt gaataagtag
     5641 ctgaatttga atttagacgg aaagagcctg atggcggggg aagcaggaaa caagtagatg
     5701 gcactcgtta actacgcggc acgcgagata aactgcaaaa tcgtttacta cggcaccggt
     5761 cttggt
//
LOCUS       DAOQNZ010000038         5761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5544_length_5761_cov_3.566071, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000038 DAOQNZ010000000
VERSION     DAOQNZ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5544_length_5761_cov_3.566071"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKW73_01235"
     CDS             <1..509
                     /locus_tag="PKW73_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71930.1"
                     /translation="GGAGKSRKRKAMTDDEAIDRGESGNLPFPASRKKLSPGLLATLS
                     STALWVLSLILAFTIHPGEPLIWLPDALLLLGFVPLLLLWHRWWLTLLFGLFNGGIGF
                     FLLLLACFKLSDFPAETRSVIGPGIEHLVGYHSCWTWMVLGVLSTAVGLVHLLVKICR
                     FLLSKSRS"
     gene            complement(522..1157)
                     /locus_tag="PKW73_01240"
     CDS             complement(522..1157)
                     /locus_tag="PKW73_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71931.1"
                     /translation="MSDRQEQTRGIRKLSNMSQVESRKCPCWQIVSAGLALTLTAGNA
                     ALANEVDQEFIVDTGSIGKSTHGVVHSDQFLELGVPTANSLRIEGEQSLRMGKLDRAI
                     MVLQRSVEMAPMDMDGRILYAEALEKKLIKQKDKRDPALYNFVVKQWLFVSKNAEFAD
                     QGMQGFQHLYKIAGTVPKKFEKEKKFLERVLIREDGTEKVILGKKPTAPNL"
     gene            complement(1174..1851)
                     /gene="rpe"
                     /locus_tag="PKW73_01245"
     CDS             complement(1174..1851)
                     /gene="rpe"
                     /locus_tag="PKW73_01245"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNA71932.1"
                     /translation="MTLIAPSILSADFARLGEEVARVEAAGADWIHVDVMDGHFVPNI
                     TIGAPVVKALRAVTKLPIDVHLMISEPDRYLADFAEAGSDYITVHQEACHHLQRTLSH
                     IRSLGKKAGVALNPATSEETLRYVLADIDLVLVMSVNPGFGGQKFLDTVLDKIDNLRR
                     MFDEAGHKDVHISVDGGINGDTGKLVVDKGADVLVAGNFIYKAKDINQAVKDLKTKSD
                     EKIKASR"
     gene            2225..3385
                     /locus_tag="PKW73_01250"
     CDS             2225..3385
                     /locus_tag="PKW73_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA71933.1"
                     /translation="MLIHKLPAELLEYQKLAKDFAAREIARVSAKHDHSGEFPIDLIK
                     SAWEAGLLNMSVPEDCGGLGLSCLEGAVIAEELGFGDSGISGSIEASDVAQRFVIEVG
                     TPEQKQEFLAPLMESPSLAGYAMDTGIRAGRVFFRRDGDDFFLNGKHNAIANGGVAEW
                     YLIKAYEDRRELAASGDASEQELVGGTYFLVRSGEDGLNFDHRLETLGKRAHVISSAR
                     FEEVMVPAGAVLLGTGQAARIYNQSLVKTYPIIAAGLVGVARAAMEHALKYSKERQTF
                     GLPISSFQGISFMLADMAKDIEAARLLVYQAAQLADQGQSCISEAVCAKAFAQDMAMR
                     VTTDAVQVFGGYGFSKEYPVEKLMRDAKVGQLTDGTSESLKVNLGRQMVTSV"
     gene            3429..3644
                     /locus_tag="PKW73_01255"
     CDS             3429..3644
                     /locus_tag="PKW73_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71934.1"
                     /translation="MDVKRGQLLKVKAPPLFEKEYLYVVTASGDKMIRADLQHSPKVK
                     KQWTHEEFQLSVKHGIIRLVDATSDNT"
     gene            complement(3641..4165)
                     /locus_tag="PKW73_01260"
     CDS             complement(3641..4165)
                     /locus_tag="PKW73_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71935.1"
                     /translation="MSGSCPYCGAKLNYGIKFCVVCGRPIQGSETGRIGGGMRSGIRP
                     ADVTRRLEDLMTAARFRRSKRQVTLDQSVHLFSLNAFSVVTGIALLFCAVKLSLDGGF
                     IHKEHKMVAPFSKILNDIGANKVNFKNGTVKTDDKSDTSNKQTGKSDSSSGKKKSGKS
                     QNKRNKNKNKQNQS"
     gene            complement(4218..4433)
                     /locus_tag="PKW73_01265"
     CDS             complement(4218..4433)
                     /locus_tag="PKW73_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71936.1"
                     /translation="MAVSELEKAFGTLTEALDLTESEITEEIKIIQQQIEELKERIVE
                     LHGKQETLAHDRESISEMVNRYAAANP"
     gene            complement(4559..5368)
                     /gene="cysE"
                     /locus_tag="PKW73_01270"
     CDS             complement(4559..5368)
                     /gene="cysE"
                     /locus_tag="PKW73_01270"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR01172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNA71937.1"
                     /translation="MYLLECIENIFEQDPAARSRLEVVLCYPGFHALVFHQIASLLWR
                     CGLRTPARYTSHWGRVLTGIEIHPGATIGRRVFIDHGMGVVIGETAVVGDDCVIYQGV
                     TLGAGAAARQGASSRDTKRHPTLGKGVVVGSGAEIQGAIEVGDNVRVASGSIVLKDVP
                     ANSIVVGVPGRVIYRDGKKVEKKEGDVPDIEAEAIKSLRDHINKLEAELLSLRSKIKG
                     QQNAIEDLKSQLPASLNPPAADASGTDYQALPGGESDKTDPVDVFLQGAGI"
BASE COUNT         1211 a         1504 c         1542 g         1504 t
ORIGIN      
        1 aaggtggcgc tggcaaaagc cgcaagagga aagccatgac tgacgatgag gctattgaca
       61 ggggcgaaag cgggaattta ccattcccgg cttccagaaa gaaacttagc cccgggctat
      121 tggctacgct ttcttccacg gccctctggg tgctgagcct gattctggcc tttaccatcc
      181 atcctggtga gccgcttatc tggctacctg atgccctttt gctcttaggt tttgtgcccc
      241 tgcttctgct ctggcaccgc tggtggttaa cgctcctgtt tggattgttc aacggcggta
      301 ttggattttt cctgctgctt ctggcctgtt ttaaattgag cgacttccct gccgagacgc
      361 gctccgtcat cggacccggt atcgaacatc tggtcggcta tcacagttgc tggacctgga
      421 tggtgctggg cgttttatcg actgcagtcg gcctggtgca cttgctggtg aagatctgcc
      481 gctttcttct cagcaagagc cgaagctgat tgagctaatt gttagagatt cggagcggtc
      541 ggctttttgc ccaggatcac tttctcggta ccgtcttcac gaatgagcac gcgttccagg
      601 aacttcttct ctttctcgaa cttcttcgga acagtgcctg ctattttgta gaggtgctgg
      661 aagccttgca tgccctgatc ggcgaactcg gcgtttttgg aaacaaataa ccactgcttc
      721 acgacaaagt tatagagtgc gggatcgcgc ttatcttttt gtttgatcag tttcttttcc
      781 agggcctcgg catagaggat gcggccgtcc atatccatgg gtgccatttc tacggatctt
      841 tgcagcacca ttatggctcg atcaagctta cccatgcgca gagactgctc gccttcaatg
      901 cgcaggctgt tggccgttgg tactcccaat tccagaaact ggtcggaatg aacgactccg
      961 tgggtgctct tgcctatgga gccggtgtcg acgatgaatt cttgatctac ttcgttggcc
     1021 agggcggcat tgcccgcagt cagggtgaga gccagaccgg cgctaactat ctgccaacag
     1081 gggcactttc ttgattctac ttggctcatg ttgctcagct tccttatgcc tctagtttgc
     1141 tcttgtctat ccgacatgct catctccttg cggttagcgt gatgccttga ttttttcgtc
     1201 gcttttggtt tttaagtctt ttacagcctg gtttatgtct ttggctttat aaatgaaatt
     1261 gcctgctaca agtacgtctg cgcccttatc taccaccaat ttgccggtat cgccgttgat
     1321 accgccatcg acggatatgt gcacgtcttt gtgcccggct tcatcgaaca tgcggcgcaa
     1381 gttgtcaatc ttatccagga ccgtgtcgag gaacttttgt ccgccgaagc ctgggttcac
     1441 cgacatgacc aggaccaggt caatgtccgc aagaacatag cggagagtct cttcgctggt
     1501 ggccgggttc aatgccacgc cggctttctt gcccaggctg cgaatgtggg aaagggtgcg
     1561 ctgcaggtgg tggcacgctt cctggtgtac cgtaatgtag tctgagcctg cctcggcgaa
     1621 atccgccagg taacggtccg gctcggatat catcaaatgc acgtcgattg gcagttttgt
     1681 tactgctctc aaggctttca cgaccggcgc accgatagta atattcggta cgaaatgacc
     1741 atccatgacg tccacatgga tccagtcggc gccggccgct tccacgcgtg ctacttcttc
     1801 acccaggcgg gcgaaatcgg cggagaggat tgagggggct atgagggtca cgatagtggg
     1861 ctttcggttt gttccaaggg tacttcttga cagagtcaat gatgacgcca gggccaggtc
     1921 ctggtcaagt ggctcctcta agcattgccg gtgccggtgg tgtgaacttt attttaatgg
     1981 cgcctgccct cgtaggtaaa agcggcgctg ttccgcacat tccaggtgtg ttcattttag
     2041 accgtggctg ccaagtggcg ttttcctgta atcataataa gatgctaata gccggcggag
     2101 aagtctgcct gggacattct aaaatagcta ttcttaagta acctggagta ctctgactgc
     2161 tgccaggaat tgcctccttc ccggtttgcc cggcacacca ccatcaaata aaagaaccag
     2221 aactatgcta attcacaagc ttcccgccga acttctcgag tatcaaaaac tggcaaagga
     2281 ctttgctgct cgtgagatag cccgggtttc cgccaagcac gaccacagtg gtgagtttcc
     2341 aattgatttg attaagtcgg cctgggaggc cggattgctc aatatgtcgg tgcccgaaga
     2401 ttgcggaggg ctcggtcttt cttgtttgga aggggctgtg attgccgagg aattgggctt
     2461 cggcgatagt ggtatctccg gcagtattga agcctccgac gttgctcagc gcttcgtaat
     2521 agaagtggga actcccgaac aaaagcaaga gtttctggcg ccactgatgg agagtccttc
     2581 tctggctggc tatgccatgg atacaggtat tcgggccgga agggtcttct ttcgcaggga
     2641 cggcgatgat ttcttcttga acggcaaaca caatgcaatt gccaatggtg gcgtggccga
     2701 atggtatttg attaaagcct acgaagaccg gcgcgaactg gccgcctctg gtgacgcctc
     2761 cgagcaagaa cttgtcggcg gcacatactt cctggtcaga tcgggcgaag atggtcttaa
     2821 ctttgaccac cggctggaaa cgcttggtaa aagagcccat gttatttctt cagcgcgttt
     2881 tgaagaagta atggtgccgg ccggcgctgt tttgcttggt accgggcagg ccgcacgcat
     2941 ctacaatcag tctcttgtga agacctatcc gatcatcgcc gccggtctgg taggagtggc
     3001 tcgcgccgcc atggaacatg ccctcaagta ttccaaggag cgtcagactt ttggtttgcc
     3061 gatttcgtct ttccagggca tctctttcat gctcgccgac atggccaaag acattgaagc
     3121 ggctcgcttg ctcgtctatc aggctgcgca acttgccgac cagggtcagt cttgtattag
     3181 tgaagccgtt tgcgccaagg cctttgccca ggacatggcc atgcgggtca ctaccgatgc
     3241 cgtgcaggta ttcggcggtt atggattctc caaggagtat ccggtggaaa aactgatgcg
     3301 cgacgctaaa gtcgggcagt tgaccgacgg caccagcgag tcgctgaaag tgaacctggg
     3361 gcggcaaatg gttacttccg tatagagttt gatcgcagcg ggaacgagca cggtaagcgg
     3421 gtgataactt ggatgtcaaa cgtgggcaac tcctgaaagt aaaggcgccg ccattgtttg
     3481 agaaggaata tctttatgtc gtcactgcca gcggcgacaa aatgattcgc gccgacttac
     3541 agcactcgcc caaggtaaag aagcagtgga cccacgaaga atttcagctt tccgtcaagc
     3601 acggcataat tcgcctggtg gatgccacca gcgataatac ctagctttga ttctgcttgt
     3661 tcttgttttt gtttctcttg ttctgtgact tgccgctctt ctttttgccg gagctgctgt
     3721 cacttttgcc agtctgttta ttggatgtat cggatttatc gtccgtctta acggtaccgt
     3781 ttttgaagtt gaccttattg gcaccgatgt cgttgaggat tttggaaaat ggtgccacca
     3841 ttttgtgctc tttatgaata aagccgccgt caagactgag cttgacagcg caaaagagca
     3901 gagcaatgcc ggtcaccacg gaaaaggcat tgagagagaa gaggtgcacg gactgatcaa
     3961 gagtcacctg ccgtttggac cggcggaatc tagcagccgt cattaagtct tccagccgtc
     4021 tggtgacgtc ggccggtctg atgccgctgc gcataccgcc gcctatcctg ccggtttcgc
     4081 ttccctggat tgggcgaccg catacaacgc agaacttaat gccgtaattt agttttgcgc
     4141 cgcaatacgg gcaagagcct gacattagtg gttatcaccg gggaacgggg ctttcaagac
     4201 aaatctcaag cgatacttta tggattggcc gcagcgtatc tgttgaccat ctccgatatc
     4261 gattcacggt cgtgagccag ggtttcttgc ttaccatgga gctcgacaat tctttctttc
     4321 agctcttcaa tttgctgctg aataattttg atttcttcgg ttatctccga ttctgtcagg
     4381 tcaagggcct cggttagagt gccaaaggcc ttctcgagct cgcttacggc catgcgagta
     4441 tccttcctcc ggtaaattgc aattgctaac gcctggagtg gcatcgaacc actcaggttt
     4501 attctaccct tctttgtccc gtctaacgct catcgccaat aagtattgct aaaccggctc
     4561 aaattccagc accctgcaag aatacgtcca cagggtcggt tttatcgctt tcaccacccg
     4621 gcagtgcctg ataatcagtg ccggaggcat cggcggcagg cgggtttaag cttgccggta
     4681 actggctctt caagtcttcg atggcattct gctgaccttt gatcttgctg cgcaggctta
     4741 agagttccgc ctccagcttg tttatgtggt ctctcaagct cttgattgct tccgcctcaa
     4801 tgtccggaac atcgccttct tttttctcga cttttttgcc gtccctgtag atgacccggc
     4861 caggcacgcc gaccactatg gaattggcag gtacgtcttt gagaacaatt gagccggaag
     4921 ccaccctgac gttgtcgccc acttcaatgg cgccctgaat ttcggcgccg cttcctacaa
     4981 cgacgccctt acccagggtc ggatggcgct tggtatctct ggatgaggct ccctggcggg
     5041 ctgcggcgcc cgctcccaga gtcaccccct ggtagatgac gcagtcatcg cctaccacgg
     5101 cagtttcacc gatgaccacg cccatgccgt ggtcaataaa gacccgccgg ccgatggtgg
     5161 ctcccggatg aatttcgatg ccggtcagca cccttcccca gtgcgaggtg tagcgtgccg
     5221 gggtgcgcag cccgcagcgc cagagcagtg acgcgatttg atgaaaaaca agagcatgaa
     5281 aacccggata gcacaggacc acctccagtc gactgcgagc ggccgggtct tgttcgaaaa
     5341 tgttctctat gcactcaagc aggtacatgg cgccttttgc ttcctttcta ctcctgatga
     5401 aacagtagca gaccacccgt tacttttaca aaagctcctc ttgataaccg ccgcgtgggc
     5461 gttctcgggt ttaggttgtc aggtctgtgg ggatataaaa gattgataga taagcgtact
     5521 aaggcagcct gaagaccctg tgcaaaacaa aagtccagcg acttcagtgg ggaaaaccgc
     5581 aaaattaagt gcggttcata gtgggttccg gctgtttcgg tcgataaaaa gatgagaaat
     5641 ttgtctaaat tcttgacagg gggtgtataa ccggcgacag cggtcaatta aacgcctctc
     5701 aggacccagt aaaaaagact caagatgacc acggataaag gaccagcgca taacgacaag
     5761 c
//
LOCUS       DAOQNZ010000039         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5629_length_5705_cov_3.686549, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000039 DAOQNZ010000000
VERSION     DAOQNZ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5629_length_5705_cov_3.686549"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKW73_01275"
     CDS             complement(<1..351)
                     /locus_tag="PKW73_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNA71938.1"
                     /translation="MNTKQAITFDPNSLGQKDSNIFGLPFSPEQAQMVLIPVPWEVTV
                     SYRAGTAGGPAAIMDASKQVDLYEPLLEDAWKIGLSMLDLPEHLLLMNSLARSKAEAY
                     ILLLENGESPETSQT"
     gene            complement(434..1573)
                     /locus_tag="PKW73_01280"
     CDS             complement(434..1573)
                     /locus_tag="PKW73_01280"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71939.1"
                     /translation="MTPKSSVIITTSCLVFGFTSLGYLVPLSIVHPAPQLLTAIALEA
                     VWFLSYLGFSLYRLKSTGGRAQDAVISELVSQVAVIEDNRKNGRRVLMAMALITWLAM
                     LIDLSAFLLAFCGQFNASAAIYRAVPISTCLGLNVAASLEVLGGAYVEAGKYPQALSI
                     YREVETIRQSHYGAVSEKMAAIFADYGDLYTKMGTYDKAETSYKTAIDLANQILGNQG
                     TGRAYTRLANLLTQIGRLPEADYYYQKALTMREKQFGKESAKVAETLFAYSVLLEKSG
                     KKPEAKLSQERAEKIYQRLKANSKTGKIDYQPLMVMAVSIFVSWLLFGKKGYLTELAV
                     RRLKNRIETGSKTSAKDLKTLALIYQYQNKKDELERLKLQNTRAL"
     gene            1937..2320
                     /locus_tag="PKW73_01285"
     CDS             1937..2320
                     /locus_tag="PKW73_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71940.1"
                     /translation="MKEEKSKAKEDNYRYGLFRWIDSIDPEIPISAHLSIAFKDSQLS
                     GAVAHGKHIVERYSYWIVFIPSIKNRDNANGSLVCLEDGGLVPYSPESLRTALKELCE
                     DTREQARRFEKLDGMVQADLVSSQR"
     gene            2756..3481
                     /locus_tag="PKW73_01290"
     CDS             2756..3481
                     /locus_tag="PKW73_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71941.1"
                     /translation="MIEGKEPNISNENYYSWHQALYSKIRDRLSVSGYSHSFFKGFDG
                     ARSFAAGGVDIPLVHCWKDPEPDDVLQIMIIRGVVDRGGMLVELQRCLGQKQVGELLC
                     EYQIAEHFSEPVRKLGLASRHFQKTHSPELIDEGLDVLFKELVNSPINATAPEKSKLF
                     NLVWRDARRDNIACLMALACAVQAGEKDKVVEFANSLKPMLAHFKVLSTQSNLLDSGT
                     NRMVERMLRQLDFAEAMLTSLDF"
     gene            complement(3498..4781)
                     /locus_tag="PKW73_01295"
     CDS             complement(3498..4781)
                     /locus_tag="PKW73_01295"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA71942.1"
                     /translation="MSELINRHLASRVEEALQISRIVNIVGPRQAGKSTLVKNQLPIA
                     EYLTMDSDSIRSSMQGDAYAMLRSITNNHKTTSLPIALDEVQHVPEISLAIKRIVDED
                     NRKGQFLLTGSANVFDLASSGDSLAGRVHTLLLRPLSVAEIHNAGPCRLLDAVESTPE
                     TIISALPVVKQHSRTAAIELILRGGYPEIRVLDDRQRLERYNGYIDSIVVKDVPVVSP
                     VRKPDLLRRLIDQLAARTAQELNMTNLCNAVGARKETVGSWLDTLERVCLIQRLPSWA
                     PSSAKRAVHWPKLHFLDTGCASALRNETQASFDFSANPASLGSMLETFVYQELEKTLP
                     LVGSHWRLAHWRSDNAEIDIVAECPGRSLALFEIKATSSVSSSDFKSIDWFLKDGPGK
                     AYGARAVGIVVYLGDQVLTMGPRKICLPLSIFWSY"
     gene            5076..>5705
                     /locus_tag="PKW73_01300"
     CDS             5076..>5705
                     /locus_tag="PKW73_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71943.1"
                     /translation="MSFEQQQFERGNSDRFSQQNSLAGSDLGDNRSFFQERRTCDNSL
                     KEEQGLLTFSQPKEFDASPFSHQRDLGLNRNDSGNFGWRHSQSQFNRESGYEDNGFNS
                     RGNDESLRDRHDHFRQFDGHEHRHHRGDQGHDSPFARKDVQKLLEEIHQIEEKLAQLL
                     KELQAGRHPSPPCGSKPGGGSPEVPTTPDTPDIPPVPSQPDSPNTPSTPD"
BASE COUNT         1413 a         1293 c         1497 g         1502 t
ORIGIN      
        1 ggtctgactg gtttccggac tctcaccgtt ttccagcaag agaatgtaag cttctgcttt
       61 gctgcgggcc agactattca tcaagagcag gtgctcgggt aaatcgagca tggaaagccc
      121 tatcttccag gcgtcttcca gaagtggctc ataaagatct acctgctttg aggcgtccat
      181 gatggcagcg ggtccgccgg ccgtgccggc gcggtaggaa accgttactt cccacggcac
      241 aggaatcaaa accatttgtg cttgctcagg gctgaaaggc agaccgaaaa tgttggagtc
      301 tttctgtccg aggctgttag gatcgaaggt aatggcttgt ttggtgttca taactgtact
      361 ttctggaaag ctctatgaca gagtttaagg tagctaagtt ttgccggttt tggctcggtc
      421 ccaccgcatt tttttataat gccctggtat tttgcagttt gagccgttcc aactcatcct
      481 tcttgttttg atattgataa atcagggcca gtgtttttag atctttggcg ctggttttag
      541 agcctgtttc tatgcgattc ttcaggcgtc gcacggcaag ctcggtaaga tagcctttct
      601 tgccaaagag aagccacgaa acaaaaatag aaacggccat gaccataagc ggctggtagt
      661 cgattttgcc tgttttactg ttggctttca ggcgttgata gattttctcc gctctctctt
      721 gggacagttt ggcttccggc tttttaccgc ttttttccag cagcaccgaa taagcgaaga
      781 gggtttcggc aactttagcg ctttctttgc caaattgctt ttccctcatg gtgagagcct
      841 tctggtagta atagtcagcc tccggcaggc ggccgatctg ggtgaggaga ttggctaatc
      901 tggtgtaggc gcggccggtg ccctggttac ccaagatctg attggccagg tcaatggcgg
      961 ttttgtagct tgtttccgcc ttgtcatagg tgcccatctt ggtatagagg tcgccgtaat
     1021 cggcaaagat ggctgccatt ttttcgctca ccgccccata atggctctgt ctgatggttt
     1081 ccacctcgcg ataaatagag agtgcctggg gatattttcc ggcttccaca taggcacctc
     1141 ccagcacttc gagagaggcg gctacattga gccccaggca ggtagaaatg ggcacggccc
     1201 ggtaaatggc ggcagaggca ttgaactgtc cgcaaaaggc aagcaaaaag gcggagagat
     1261 caatgagcat agccaaccag gtgattagtg ccatggccat gaggacgcgc cggccgtttt
     1321 tgcggttgtc ttctatgacc gctacctggc tgaccagttc gcttataaca gcatcttgcg
     1381 ctctgccgcc ggtgcttttg aggcgataga ggctgaagcc caggtaactc aaaaaccaga
     1441 ctgcctccag ggcgatagcg gtgagcagtt gcggcgccgg atgcacgata gaaagcggca
     1501 cgagatagcc gaggctggta aaaccaaaga ccaggcagct agtagtgata atgacgcttg
     1561 attttggggt cacgagaggc cggtcttctg ttgccctggg ttctgtaggt ttttgggtgc
     1621 ttcttgggct tgcaggtctg acccaagcct tcatagtagg cacattgtag tttcgttaga
     1681 agacggtttc taccaccccg ggcggagttt ttcgccgact tgggcgaggg tgaatcggcc
     1741 gggcgggtac attcatatgt ggttaattgt agatggggct ttgatggttg atctgggatt
     1801 tcttcagaag ctttgtctcg gtgcagtagt tgtcgtagtt gcactagcgc ctacttgtgc
     1861 attggcggga gatggaccga gctcatgtaa acctgcaaag cgagcaaatt gcgagatggt
     1921 tcttctattg aagtttatga aagaggaaaa gtctaaagca aaggaagata attacagata
     1981 tggactattc aggtggattg atagcattga tcccgaaata ccgataagcg cccatctttc
     2041 tatagcgttc aaagacagtc agttgtctgg agctgtggct catggaaagc acattgttga
     2101 aagatactct tattggattg tgttcatccc ctccatcaag aatcgcgata acgccaatgg
     2161 tagcctggta tgtttagaag atggcggtct tgtaccatac agtccggagt ctttgagaac
     2221 tgctcttaaa gagctgtgtg aagacactag ggagcaggca cggagattcg agaagctcga
     2281 cggaatggtg caggcggatc tcgtctctag ccaaagatga aaggaagagt tagagatcgg
     2341 cagcagcagg atctgtcttg tcaccagtgg cataagcaaa ggagatcacc tgtatgtcta
     2401 aagaaatctg ccgatgggcg agtcaaattg ttctttgacg gaggatttgt ttgaaacgaa
     2461 cacttgtgtg cttggcattt acaatatctt ctgcgctggc aatagagata gcctttgtga
     2521 gcgtaactgg ttgtgaggaa gcgcttgcac cggtacttcg gaacatctat gccggggcga
     2581 gtcttacgga tagcgatcgg ggaatctttg ccaggaagct gctccagtgc tctccttatt
     2641 ttcttgtacc gggagtaata ctgggcttag ttctttccag aaatccgaag agcaaggtca
     2701 gaactgctga gtcagggcga gttgagcaat tggaaaaacc atgacggagt cttaaatgat
     2761 cgagggtaaa gagcctaaca tcagtaacga gaattattat tcgtggcatc aagcgctcta
     2821 ttcaaaaatt agagacaggc tctcagttag tggttattct cattcgttct ttaaaggctt
     2881 tgatggtgcc aggtcctttg ctgccggtgg cgtagatatt cctcttgttc attgctggaa
     2941 agaccctgag ccggatgatg tcttgcagat tatgatcatc aggggagttg tagatagggg
     3001 tggcatgctt gtggaacttc agcgctgctt gggacagaag caagtcggtg agttactttg
     3061 tgagtatcaa attgctgagc acttttcaga accagtgcga aagctagggc ttgccagtcg
     3121 tcactttcaa aagactcatt caccagagtt gattgatgaa ggattagatg ttcttttcaa
     3181 agaattggtc aatagcccca taaacgcgac tgcaccagag aagagcaaac tattcaattt
     3241 ggtttggcgt gatgccaggc gagacaacat cgcctgcctt atggcactgg cttgtgccgt
     3301 tcaggctggg gagaaggaca aagtagtcga atttgcgaat tcgcttaagc cgatgcttgc
     3361 gcattttaag gttctttcaa ctcagtctaa tctgttggat tcgggcacga accgcatggt
     3421 tgagaggatg cttcgacaat tggattttgc cgaggccatg cttaccagtc ttgatttcta
     3481 atcattctct tttttcatca gtaggaccag aaaatagaaa gcggcaggca gatttttcta
     3541 gggcccatag tcagtacttg gtctcccaga taaactacaa ttccgaccgc tctagcgcca
     3601 taggctttac cagggccatc tttcaggaac caatcaatcg atttaaaatc agaggatgat
     3661 actgaagatg ttgccttaat ttcgaacaag gctaagctgc ggcctggaca ctctgcgact
     3721 atgtctatct cggcattgtc agaacgccag tgggccagtc tccaatgact gccgaccagg
     3781 ggaagagttt tttcaagttc ctgatagacg aatgtctcaa gcatagagcc cagcgatgcc
     3841 gggtttgcac tgaaatcaaa actagcttgt gtctcatttc gcagtgctga agcacagcca
     3901 gtatcaagga aatgcagctt tggccaatgc acagcgcgct ttgctgaaga tggcgcccat
     3961 gatggcaatc gctgaatcag acaaaccctt tctagtgtat cgagccaact tcctacggtt
     4021 tctttgcgag caccaacagc attgcagaga ttggtcatat ttagttcttg tgcagtgcgt
     4081 gccgccaact gatcaatcag gcggcgcaac aagtctggtt tgcgcaccgg agatacaaca
     4141 ggcacgtcct ttacgacaat gctgtcaatg tagccattgt agcgctcaag tcgctgacga
     4201 tcatccagaa cacgaatctc cggatacccg cctctcaaga ttagctctat ggcagctgtt
     4261 cttgaatgtt gtttgacgac gggcagtgct gagataattg tttctggtgt actttcaaca
     4321 gcatcgagca aacgacaagg gccggcattg tgtatttccg ctacgctgag cgggcgcagc
     4381 aggagcgtat gtactcgccc tgctaaagag tctccactgc tggctaaatc aaagacgtta
     4441 gccgagcctg tcagcaagaa ttgacccttc ctattatcct cgtcaacgat gcgctttata
     4501 gcaagcgaga tctcagggac gtgctgcacc tcatcaaggg caatcggcaa tgaagttgtc
     4561 ttatggttgt tggtgatgct gcgtaacatg gcataggcat cgccttgcat agagcttctg
     4621 atactgtccg agtccatagt caggtactcg gcgatcggca gttgattttt gaccagcgta
     4681 cttttgccgg cctgacgtgg tccaactatg ttgacaatgc ggctaatctg cagtgcctcc
     4741 tcgactctag aggctaaatg acggtttatt agctccgaca tcgacagcta tcccaaatta
     4801 ccactcgaga aatagtctag caggaagtcc aaatattggc tagtagatag gcagaaaatt
     4861 tactagctac tagctaattt ctcggattcc cggcaaaagg gctggacgaa aggggttttg
     4921 ccggcggcat gcttacccta cttgatttct aatccggcaa acactgtgtc gacacaattg
     4981 aacagcacca taggtgaacc gaccaggaca ggcggtttac taattcccac aagcttttgc
     5041 tttgattcca actcactccc agaaggaacc aagagatgtc ttttgagcag caacaatttg
     5101 aaagaggaaa ctctgatcga ttttctcaac aaaatagttt ggccggctct gatttgggcg
     5161 acaacaggtc gttttttcag gaaagacgta cttgtgacaa ttcgctgaag gaagaacagg
     5221 gtcttctgac attttctcaa ccgaaggagt ttgatgcttc accgttttca catcaaagag
     5281 atttaggact caatagaaac gatagcggca atttcggctg gagacactca cagtcgcaat
     5341 tcaatcgaga gagcggctat gaagataacg gtttcaactc cagaggaaat gatgaaagtt
     5401 tgcgtgatcg ccatgatcat ttcaggcaat tcgatggtca tgaacacaga catcatcgtg
     5461 gtgatcaagg tcacgattcg ccgtttgctc gcaaagatgt tcaaaagctc ttggaggaaa
     5521 ttcatcagat cgaggagaaa ctagcccagt tattgaagga attgcaggct ggtagacatc
     5581 cctcgccgcc atgcggatct aaaccaggcg gcggcagtcc ggaagtacca actacgcctg
     5641 atacgccgga cattcctcca gttccaagcc agccggattc accgaatact cccagcactc
     5701 cagat
//
LOCUS       DAOQNZ010000040         5672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5668_length_5672_cov_3.207762, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000040 DAOQNZ010000000
VERSION     DAOQNZ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5668_length_5672_cov_3.207762"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PKW73_01305"
     CDS             <1..1111
                     /locus_tag="PKW73_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNA71944.1"
                     /translation="ELSYSATFGVLVLLPLVQGSFDYFFEKLPAFLRLPLLQLFLRLL
                     AVVLAAQSAVMPLQLFYFGQFSTLFLPANLLAEPLVAPITVLGFASTIFACPLLMLPP
                     GLVPACAYLPSLCLDWLSLPFLQALNALSRMLSTWSGCILTVGRPACSQLLFFYLVAL
                     ALPALQPGLSRFLRWTTATTQAFLGAFFLFFSAAFLAFFLVFSPSLEILTTSQGFIVR
                     RAGQGLYCSGIDITGDTTNAAGGPCSTLASFLKARLEPRFPVTMSAPGAEAVCLSLQP
                     QECYRIALAKDGLVILGHRPVGSRRPFEISIFQVGASTARYQLHYSPNNGWHETGSIA
                     FPACLPYFELALPVYPVLESERECRSSSCLVRFRI"
     gene            complement(1173..2456)
                     /locus_tag="PKW73_01310"
     CDS             complement(1173..2456)
                     /locus_tag="PKW73_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71945.1"
                     /translation="MQQVRTGTQQQQEFVPPYPASIAETGMKEGFLEELVLKDIYMVN
                     FALGREIASRTMLPFKIVEEIIEVLKKQLLIEVRSSGGLADYEYTPTEKGRDRARAYF
                     DHNAYVGPCPVPWSRYVDSLKYQTIRREHVTPRDLEVAFSDIMVNRRTINAVGPAINS
                     GRGMFLFGEAGNGKTSIAERIVRSFKGHIFIPYAVEIDGQIIRVYDNHNHEQVSAANY
                     PRDYDHRWVRIQRPCVVVGGELTIDMLELQFDYGTKLYEAPIQLKSNCGLLLIDDFGR
                     QRIDHKSLLNRWIVPLEKRVDYLTLHTGKKLEVPFEQLIVFSTNLNPADLVDEAFLRR
                     IPYKINITNPTEDEFKWIFLQYCQRTGVPYNEEAVNYLIESQYRSGKRMMRCCHPRDI
                     VDQVINLCAFLQTDPRLSREYLDHACAVYFAAMGK"
     gene            complement(2689..5283)
                     /locus_tag="PKW73_01315"
     CDS             complement(2689..5283)
                     /locus_tag="PKW73_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNA71946.1"
                     /translation="MNNLPKKNKKKEEAPESSLGTRLTALTMNLVLLFGSACYIPTPP
                     LVTLLFMMTAILGSYLAYLFRDSRPIWLGTLPTVGTLLLFTNFGFEIYAVSMSSSGTA
                     VAAFVHLIAGLSALHCFDLRTRTDFSIASLIGLALLTFLTGVGGKDPFFGAFILSYTI
                     LAGLLLFFDSSSRSHELGPSRAVAGGDSKKQVMEKRLRAISLTSFIPVILLPLFSFGT
                     FVVLPRTDSLIDVFIDNVVRSKFPLSAYLSGQLGKGGRSQALRGGVDGQKEGGSGFTV
                     KGGEHGTAGGAGKAADNPKSSNQATTAPLGQGQENIDLSKIKGTLNAQQMAKLKEQLK
                     RDKTLNEAYQKEGIDLSNPQTNAETLIMKVATPQHLYIRKYALNGFDGTLWKRALPTT
                     PRVIKANKTFGFDLTASDTIYVPPALPTLEVKQDFRAETDLGYIVPTSWIPQIVKVGG
                     EELRMDSDATVKATEPIKRGAAFTVISQAPVYDMQVMRNHPLETLLELDEDRKDEYET
                     AASCLKHGGGLTSGIKELSQKICNDPEANWFSRAEKIQEYLKKNYQYENDGLFKTAPS
                     TENPAQAIDNFLFKSKTGSCRHFATAHALLCRAQGIPARLVVGYLPGTFNKATGYNEI
                     RAKDSHVWTEVYIPYWNWVPFDATPQGQLPAHEEGGNVLSKFIRSGLANPFAQEIKSN
                     KKPRRNLIGLEGLGQNSDGKPEAPPLPGADDKSKMLNLPFLGTVDPAVMQLTAKIAGV
                     IFALALLVVAILLYLRYRKEAEVKQLLLTHKASTVIYLDVLNELKRYEIVKFPTETVD
                     EISFRLSERFQTLADEGKPVPKQLEPVVSSFMQLYNEDRFGGIDNLDELTEIAEQIKA
                     LAAGSSKS"
     gene            complement(5280..>5672)
                     /locus_tag="PKW73_01320"
     CDS             complement(5280..>5672)
                     /locus_tag="PKW73_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71947.1"
                     /translation="RVRVPQVGMSDTLALLTVRPSDLATQPEEEFKAGEPELDESKDP
                     LARLTAATMRPAVSASALRQVDLWVLSRAFLEADSTLMMARQASAPIPLHAQGSGDRR
                     SSPMTRDKPAQYGRILASLAEFEELAHL"
BASE COUNT         1075 a         1502 c         1638 g         1457 t
ORIGIN      
        1 agagctttct tacagcgcta ctttcggcgt actggtgcta ctgcccctgg tgcaaggcag
       61 ttttgactac ttctttgaaa agttgcctgc tttcctgcgt ttaccactgc tgcaactgtt
      121 cttgcgactg cttgccgtgg tgcttgccgc tcagtcagcc gtcatgccct tgcaactctt
      181 ctatttcggt cagttcagca ccttgttctt gcctgccaac ttactggctg agcccctggt
      241 tgcccccata accgtgcttg gtttcgcttc taccattttt gcttgtccac tgcttatgct
      301 tccccccggc ctggtgccgg cttgtgccta tttaccgtct ctttgtctgg actggctcag
      361 cttaccgttc ctgcaggcct taaatgcact cagccggatg ctttctacct ggagtggttg
      421 catcctcacc gttggccgtc ctgcttgtag tcagttgctc ttcttttatc tggtggctct
      481 ggcgctgccg gctcttcagc ccggtcttag tcggtttttg cggtggacca cggccacaac
      541 tcaagccttc cttggtgcct tcttcctctt tttcagtgcc gctttcctgg cattcttcct
      601 ggtcttctct ccctcacttg aaatactgac caccagtcag ggttttatag tgcgccgggc
      661 tggtcagggg ctttactgct ccggcattga tattaccggt gataccacta atgccgccgg
      721 cggcccgtgc tctacactgg cgtccttcct caaggcacgc cttgagcctc ggtttccggt
      781 gaccatgagt gcgccgggag ccgaggcggt atgcctgagc ctgcaacctc aagagtgcta
      841 tcgcattgcg ctggcgaaag acgggctggt gattctgggc catcgcccgg taggcagccg
      901 gcgcccgttt gaaataagca ttttccaggt cggggcaagc accgctcgct accagctcca
      961 ctacagccca aataacggct ggcacgaaac cggcagcatc gctttccctg cttgcttgcc
     1021 ctacttcgag ctcgctctgc cggtctatcc ggttcttgag agcgaacgcg agtgtcgcag
     1081 ttccagctgt ctggtgcggt ttcgtatttg attgccccaa gcaaaaagcc ctccccggaa
     1141 gaggagggct ttcttctgtt tttgaccttg gcttacttgc ccatagcggc aaagtaaacg
     1201 gcgcaggcgt ggtcgagata ttctcttgaa agtctcgggt ctgtctgcag gaaggcacag
     1261 aggttgatta cctggtctac gatgtcgcgc gggtggcagc aacgcatcat gcgtttgccg
     1321 cttctgtatt gagattcgat caggtagttg accgcttcct cgttgtaggg aacccctgtg
     1381 cgctgacagt attgcaggaa gatccacttg aactcgtctt ccgtggggtt ggtgatattg
     1441 atcttgtagg ggatccttct caggaaggct tcgtctacca gatcggcagg gttcaagttg
     1501 gtggagaaca cgatgagctg ttcgaaaggc acttccagtt ttttaccggt gtgcagggtc
     1561 agatagtcga cccgcttttc caacggtacg atccagcggt tgaggagtga cttgtggtcg
     1621 attctctgtc tgccaaagtc gtcgattagt agcagaccgc aattactctt gagctggata
     1681 ggcgcttcat agagcttggt accgtagtcg aactgcaatt ccagcatgtc gatggtcaac
     1741 tcaccgccca ccaccacgca ggggcgttgg atacgaaccc agcggtggtc gtagtctctc
     1801 ggatagttgg ccgccgaaac ctgctcgtgg ttgtggttgt cgtagaccct gatgatctga
     1861 ccgtcaatct ccacggcgta gggaatgaag atatggcctt tgaaggaacg cacgatgcgc
     1921 tcggcgatag aggtcttacc gttaccggct tcaccgaaga ggaacatccc tctgccggag
     1981 ttgattgccg gaccgacggc gttgatggtt cttctgttga ccatgatgtc tgagaatgcc
     2041 acttccaggt ctctgggggt gacgtgctcg cgacggatgg tttgatactt gagtgagtcg
     2101 acgtaacggc tccacggtac aggacacgga cccacgtagg cgttgtggtc gaaatatgct
     2161 ctcgctctat cgcgaccttt ttctgtgggc gtgtattcat agtcagccag accaccggag
     2221 cttcttactt cgatgagcaa ttgcttcttc aagacttcga tgatctcttc aacgatcttg
     2281 aagggcagca tggtacgcga agcaatctcg cgaccaaggg cgaagttgac catgtatatg
     2341 tctttgagca ccagctcttc caggaagcct tccttcatgc cggtttcagc aatgctggcc
     2401 ggatatggag gaacaaactc ctgttgttgc tgcgtccccg ttctaacctg ttgcatgtct
     2461 atctctctta tgtggagcca ctgtcgattt tcaaatagaa agcttggtcg tggcgcttga
     2521 cttccgggct tgacttctgc ttccctgggc aagattgccg gcccaagttt ttagccttgt
     2581 aagcatagca aatataacca ctttagcgcg ttgtgccggt tcttactagc acctgaaagg
     2641 cgtttgccgc ttaaatagta aatacgaagg gatttttagc cgtgggggct aagatttact
     2701 gctgcctgcg gcaagggctt tgatttgctc agcgatttcc gtcaactcat ccaggttatc
     2761 gatgcctccg aaacggtctt cgttgtagag ctgcatgaag ctgctgacaa ccggctccag
     2821 ttgcttgggc acgggtttac cttcatcagc cagggtctga aagcgctccg ataacctgaa
     2881 agaaatttcg tccacggttt ctgttggaaa cttcactatc tcgtaacgct tcagctcgtt
     2941 caagacatcc aggtaaatta cggtggaggc tttgtgagtg aggagaagct gcttcacctc
     3001 ggcttccttg cggtagcgca ggtagaggag aatagccacc accagaagag cgagggcgaa
     3061 gatcacgccg gcaattttgg ccgttaactg catgactgcc gggtctaccg tgcccaggaa
     3121 gggcaggttc agcattttgc tcttgtcgtc cgctcccggc aggggcggcg cttcgggctt
     3181 gccgtcgctg ttttgaccca gtccttccag tccaatcagg tttcgccggg gctttttgtt
     3241 tgatttgatc tcctgggcaa aggggttggc caggccgctg cggatgaatt ttgagaggac
     3301 attgccccct tcctcgtgag ccggcagttg cccctggggg gtggcatcga aaggcaccca
     3361 gttccagtaa ggaatataaa cctcggtcca gacatgggaa tctttggctc gaatctcgtt
     3421 gtagccggtt gctttattga aggtgcccgg caaataaccc accaccaggc gggccggaat
     3481 gccctgggcc cggcagagca gggcatgggc tgtggcaaag tggcgacaac tgccggtctt
     3541 gcttttgaag agaaagttgt ctatggcttg agccgggttt tcagtgctcg gggcggtttt
     3601 gaagagcccg tcgttttcgt actgataatt tttcttgaga tattcctgta ttttttctgc
     3661 gcgggagaac cagtttgctt ccgggtcatt gcagattttc tgggaaagtt ccttaatccc
     3721 gctcgtcagt cctccgccat gcttcaggca gctggcagcc gtttcatact cgtctttgcg
     3781 atcctcgtcc agttcaagga gggtttcaag cggatgattg cgcatcactt gcatgtcgta
     3841 aacaggggcc tggctgatga cggtgaaggc ggcgccgcgc ttgatcggtt cggtggcttt
     3901 aacggtagcg tccgagtcca ttctcagctc ttcgccgccc accttgacga tttgagggat
     3961 ccaactggtg gggacgatgt agcccagatc cgtctcggct ctgaaatctt gctttacttc
     4021 cagggtggga agggccggcg gcacgtaaat ggtatcggat gcggtgaggt cgaaaccgaa
     4081 agttttgttg gctttgatga cgcgtggcgt ggtgggcagt gctctcttcc agagtgtgcc
     4141 gtcgaagccg ttcagggcat atttgcggat atagaggtgc tgaggggtag ccaccttcat
     4201 tattagtgtt tcggcattgg tctgcggatt cgacagatcg atgccttcct tctgataggc
     4261 ttcgttcagg gtcttgtcgc gtttgagctg ctctttcaac ttggccattt gctgggcgtt
     4321 gagggtgcct ttgatcttgc tcaaatcaat gttctcttgc ccctgcccca gtggtgcagt
     4381 ggtggcctga tttgaactct tgggattgtc ggctgctttc cctgcaccac cagcagtgcc
     4441 atgctcgccg cccttgacgg tgaagccgga gccgccctct ttctgaccat cgacgccgcc
     4501 tctgagagcc tgactgcgtc cgcctttacc cagttgtccg ctcaaatagg ccgagagggg
     4561 aaacttggag cgcactacgt tgtctatgaa aacatcgatg agactgtcgg tgcgaggcag
     4621 caccacgaag gtgccgaagc taaagagggg tagcagtatc accgggataa acgatgttag
     4681 agatatggcg cgcaggcgct tttccatcac ttgctttttg ctgtcgccgc cggctaccgc
     4741 tcttgaaggt cccagttcgt gggagcgtga ggatgaatcg aagaagagaa gcagaccggc
     4801 aagaatcgtg tagctgagga tgaaggcgcc gaaaaaggga tctttcccgc ctacgcctgt
     4861 gaggaaggtc aacaaggcca gcccgatcaa ggaggctatg gagaagtcgg tgcgggtgcg
     4921 caaatcgaag cagtgcaggg cggaaagacc ggcgatcaaa tgcacgaagg cggccacggc
     4981 ggtgccgctt gagctcatgg aaacggcata gatctcaaag ccgaagttgg taaataaaag
     5041 cagggtgccg accgtgggca gggtaccgag ccagatcggt ctggaatcac ggaagaggta
     5101 agccaggtaa ctgccgagga tggcggtcat catgaagagc aaggtgacga gcggcggtgt
     5161 cgggatatag caagccgagc cgaagagcag caccaggttc atggtcaggg ccgtcaggcg
     5221 cgtgcccagt gaactttccg gcgcttcctc tttcttcttg tttttcttgg gaaggttgtt
     5281 cacaggtggg caagctcctc aaactctgcc agggacgcca gaattctgcc gtactgagcc
     5341 ggcttgtccc tggtcatcgg tgaactcctc cgatcaccgg agccctgggc gtggagagga
     5401 attggtgcgc tggcttgtct ggccatcatg agggtgctgt cggcttccag gaaggcccgg
     5461 gacaagaccc agagatcgac ctgcctcagg gccgaagccg agactgccgg tctcatggtg
     5521 gcggccgtaa gtctggctag tgggtctttc gactcatcca attcaggttc tcctgcctta
     5581 aattcctctt ccggctgtgt agcgagatcc gaggggcgca cggtgagcag agccagagta
     5641 tccgacatac cgacctgggg cacccgcacc cg
//
LOCUS       DAOQNZ010000041         5614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5774_length_5614_cov_3.344486, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000041 DAOQNZ010000000
VERSION     DAOQNZ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5774_length_5614_cov_3.344486"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKW73_01325"
     CDS             complement(<1..556)
                     /locus_tag="PKW73_01325"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNA71948.1"
                     /translation="MIPQSFIDAWREAAPWSSDLQVEQDLVVSRAVAELFADAGLHEL
                     LAMRGGTVLNKFFFGGGSRYSEDIDLVKVGTGKAGPIFDIIRKRLDPWLGSPKSELSD
                     ASIKLVYRFNSEADPAVKMRLKVEVNTRENFSVLGYRSLPFEVNSAWFSNRVHVKTFA
                     LNELLGTKIRALYQRRKGRDLFDLW"
     gene            complement(553..1335)
                     /locus_tag="PKW73_01330"
     CDS             complement(553..1335)
                     /locus_tag="PKW73_01330"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HNA71949.1"
                     /translation="MSISKYIDGLQARGQYWFSYQMLEAETEKTAKAVERSLSRLREH
                     GTIASPRKGFYVIVPPEYRAAGCLPANWFIDHLMKFINEPYYIGLLSAAELFGAAHHR
                     PQELQVISNRARRPVECGRARIRFFAKRYIERTPTTSHKVRTGYMRISTPEATALDLV
                     RYSFELGGLDSVAVVIAELSETMKPRELLKAAESFELSVVQRLGYILDKIERGALSDP
                     LSKWLMQQRPRLVLLRTERSSEQSQINERWRLKVNDDIEVEA"
     gene            1808..3163
                     /locus_tag="PKW73_01335"
     CDS             1808..3163
                     /locus_tag="PKW73_01335"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: protein motif:HMM:NF008570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HNA71950.1"
                     /translation="MVQDLEKAKAKSRQVLDFIAKPQLNEDERKTVIGESVQYWADHV
                     NAGFLQYRKSVSTDYTAVEWSDEGSTFRDINGKEFIDMLGGYGVYNVGHRHPKVLKAV
                     KEQLDKQAIHSQELIDPLRTYLAHLVSLITPGDLKYSFFSNSGTESVEGCLKMAMLAT
                     GRRHFIGTIGAFHGKSLGSLGGTSKAVFREPFLPLLNWSHVPFGDLDALETIFKCCDF
                     SGDRIAAFVVEPLQGEGGINVAPPGYLAGARALCDKYGAMLVFDEIQCGMGRTGKMFY
                     CEYDGVTPDLMALGKAFGGGVMPIGAVVGTEKTWAKYIENPFLHTTTFGGNPLSCAAA
                     IATISVLLEEELPRQAKEKGDYMIPRMNELVKKYPDVMKEIRGVGLMLGMEFTGNDTG
                     YAVAKNLFSRNILISGTYINSKCLRVEPPLTISYEQIDVFLKALEESVKEVSKLATAA
                     K"
     gene            complement(3195..4658)
                     /locus_tag="PKW73_01340"
     CDS             complement(3195..4658)
                     /locus_tag="PKW73_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71951.1"
                     /translation="METFQNKLARLLRKVVDRLESNGTQEPVAGQSSEEVQAPAAPHG
                     ALKDASQASAAPIYVANAVPQETERFSPSEPDSLAESEKMSRSFSRFARPGAGEITAL
                     PPSSLPSLSQDKGLQSFGVSQEKQKDISIDTTIHDRDEPEPGESLFVSDFHIDQESLV
                     FTERATSEVCMATSDSFRQVAENPHTASTTLNWLAGQLSAEVRAAVAGNPSTPQETLR
                     RLAGDDDADVRIKVANNRKAGSDILRQLANDKNKVVAGEARSMLVKKLKTCEMDNTGA
                     NLLATHKFKPTGNHLTTTYSSLDAVPSGESGTTNAASDIDKGTASGEWHALNTEVAKS
                     AYKQQKDTVKDLKFNPAGVYNRTGPQNEERNIVSASANDTIAFLTMVAARPTTPPSRL
                     LELAHHENESVRKAVAENVGSPSEAFFLLSKDKAREVRLRLAENSSCPLDVARQLEND
                     VDPFVAYEARKHLRRTSQTSGMESSEAAPSRDSGGQP"
     gene            5090..>5614
                     /locus_tag="PKW73_01345"
     CDS             5090..>5614
                     /locus_tag="PKW73_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71952.1"
                     /translation="MKCETIPELERFLNIQEQALGCNSPEVANTITRLAELYFSKGLL
                     EDAEALYRRALNIREQALGPHRVEIEDTKKNLEKVLTRKRAPSQSRTPQSMTDSSTSL
                     DAVPASMALGWPSAFDQDKEKEFEVELASLRHMMGREHPQVADCLTRMADQYCRNKRY
                     REMEPLLQEALKIRE"
BASE COUNT         1281 a         1410 c         1516 g         1407 t
ORIGIN      
        1 accaaagatc aaacaagtct cggccctttc ttcgctgata gagagcacga atctttgtgc
       61 caagcagttc gttcaacgca aatgttttga catgcaccct gttcgaaaac caagcagaat
      121 tgacttcaaa cggcaggctc cggtagccga gcacagagaa gttttctctt gtattcacct
      181 ccacttttag gcgcattttt acggcaggat ctgcttctga gttgaagcga taaaccagct
      241 taatgctagc gtctgaaagt tcacttttcg gtgagccgag ccaaggatcg agtcgttttc
      301 taatgatgtc aaaaattgga ccggccttcc cagtgccaac tttgactaag tcaatgtctt
      361 ccgaataccg actgcctcca ccaaagaaaa atttgttcag gaccgtaccg ccacgcatcg
      421 ctaggagttc atgcagtcca gcatcggcga ataattctgc cactgctcgc gatactacta
      481 aatcttgttc cacttgtaga tcactgctcc acggagccgc ctcgcgccaa gcatcaatga
      541 aagactgagg gatcatgctt ccacctcaat gtcgtcattg acctttaatc tccacctctc
      601 gttgatttga gattgttcgg acgacctttc tgtgcgcagg agtaccagtc gcggacgctg
      661 ttgcattagc cactttgata acgggtcgga taatgctcca cgctctatct tgtccaaaat
      721 gtaccctaat cgttgcacca ccgagagttc aaatgactcg gcggctttaa gcagctcccg
      781 cggtttcatc gtctcactaa gttcggcaat aaccacggcc accgaatcca gtccacccag
      841 ttcaaaggaa taccgaacga gatccagtgc cgtagcttcc ggcgttgaaa tccgcatata
      901 tccggttcga actttatggc tcgtcgtcgg cgttctctcg atatatcgct tggcgaaaaa
      961 tcgaattcga gctctgccac actctaccgg acgacgcgcg cgattgctga ttacttgcaa
     1021 ctcctgcggg cggtggtgag ccgcgccgaa tagctccgct gcactcagca aaccaatgta
     1081 gtagggttca ttgatgaact tcatcaggtg gtctataaac cagttggccg gcaaacaacc
     1141 tgccgcccga tattctggcg gaacgattac gtaaaagcct tttcgcggac tcgctatggt
     1201 gccgtgctcg cgcaatctac ttaaagaccg ttcgaccgcc tttgcagtct tttcagtttc
     1261 agcttcaagc atttggtagg agaaccaata ttgtccgcgt gcctgtaaac cgtcaatata
     1321 cttggaaata gacattattg cacctggcat tctggttgcg tagccccccg gaagaagggg
     1381 gttactgcga tcggtaccgt tgtgattata cccctaattc gggggggtag cgcaatcgag
     1441 ttgtctaggg tcgccgggtg ccttgagagg aaggagttac cggcactgga gataacggcg
     1501 caatcaacat aaacaacgag taagaccgta gctttgacct tttttggaaa gccgcattat
     1561 ttcgggccgc gatgaaaact caaggcgata gacaaagaag cctgagggac cttccgaacc
     1621 atgtaatctg gaagcccaat tcccctctcc gactagaaag agccaattgc ggcgaccttt
     1681 gacagctttg ggagttttgc ttaatgcttg acaatcgtga aggtagtaaa atttagcctt
     1741 caccccattc cgcaggtcta aagtgatgcg gaacccgagg ggtcaaaaaa ctctgggagt
     1801 aaagataatg gtccaagatc tggaaaaagc caaggccaaa tccaggcagg tgcttgattt
     1861 tatagccaag ccccagctaa acgaagacga gcgcaagaca gtcataggtg aatcagttca
     1921 gtactgggct gatcacgtca acgccggctt cctgcaatat cgtaaatcag tcagtaccga
     1981 ctataccgcc gtggaatggt ctgatgaggg ttctactttc cgtgacatta acggcaaaga
     2041 gttcatcgat atgctcggcg gttacggcgt atacaacgtc ggccaccgcc atccgaaagt
     2101 gctgaaggct gtaaaagaac agctagacaa gcaggcaatc cactcccagg agttgattga
     2161 tcccctgcgt acctacctgg ctcacctggt ttccttgatt acaccgggcg atttgaagta
     2221 ttccttcttc agcaattccg gtaccgagtc ggtggaaggc tgcttgaaga tggctatgct
     2281 agccacgggg cggcgccact ttatcggcac catcggcgct tttcatggca agagtttggg
     2341 ctccctgggt ggtacctcga aagcggtctt tcgcgaaccg tttttaccgc tgctcaactg
     2401 gtcgcacgtt cctttcggcg atctcgatgc ccttgagacc atcttcaagt gctgtgattt
     2461 ctccggtgat cgcatcgctg cttttgtggt ggagcccctg cagggcgaag gcggtatcaa
     2521 cgtagcgcct cccggctatc tggccggcgc tcgcgctctt tgcgataaat atggcgccat
     2581 gctcgtgttt gacgaaattc agtgcggcat gggtcgcacc ggcaaaatgt tctactgcga
     2641 gtacgacggt gtaactcccg atctgatggc gctcggcaag gcttttggtg gcggtgtcat
     2701 gcccatcggt gccgtggttg gaactgagaa gacctgggct aagtatattg aaaatccttt
     2761 cttgcacacc accacttttg gtggcaatcc cctgagctgt gcggcggcca tcgccaccat
     2821 ttcagttctc ttggaagagg aattgccaag gcaagccaag gaaaagggcg actatatgat
     2881 tcctcgcatg aacgagctgg tgaagaaata tccggacgtc atgaaggaaa tcagaggggt
     2941 tggtttgatg ctgggcatgg aattcaccgg taacgatacc ggctatgccg tggctaagaa
     3001 tctcttctcg cgcaatattc tcatttcagg cacctatatc aattccaagt gtttgcgagt
     3061 ggagccgccg ctcacaatca gctacgagca aattgacgtc ttcctcaagg ctctggaaga
     3121 gtcagtaaag gaagtgtcca aactggctac cgctgctaaa taaagggcgg tattccgaac
     3181 tcaggagtca gggctcaggg ctgcccgccg ctatcgcggc tgggcgctgc ttcgctgctt
     3241 tccatgccgg atgtctggct ggtgcggcgc agatgcttgc gagcttcata agctacaaat
     3301 gggtctacgt cattttccag ttgtctggcc acatccaggg ggcaactgga attttccgcc
     3361 agcctcagcc ttacttcgcg ggctttgtct ttggagagca ggaagaaagc ttcactgggg
     3421 ctgcctacgt tttcagccac agccttgcgt accgattcgt tttcgtggtg agctaattct
     3481 aaaagccgtg acgggggagt ggtggggcgg gctgcgacca tggtcaaaaa ggcgatagtg
     3541 tcattggccg aggctgaaac tatgttgcgt tcttcgtttt gcgggccggt gcgattgtag
     3601 acaccggcag ggttgaattt gagatctttg accgtatctt tctgctgttt gtaggctgat
     3661 ttagctactt ccgtgttgag ggcatgccac tcgccggatg cagtaccctt atcaatgtcg
     3721 gaagcggcat tggtggtacc gctttctcca gaaggtactg catcgagcga cgagtaggtg
     3781 gtggtgaggt gattaccggt tggtttaaac ttgtgtgtgg cgagtagatt ggcgccggtg
     3841 ttgtccattt cgcaggtctt gagctttttg accagcatgc tgcgggcttc tccggcaaca
     3901 actttgtttt tgtcgttggc cagttgtctc aagatgtctg aaccggcttt gcgattatta
     3961 gcaactttga ttcgtacatc ggcatcatcg tcgccggcca gcctgcgcag ggtttcttgc
     4021 ggagtgctgg gattgccggc aacggcagcg cggacttcgg cggaaagttg accggccagc
     4081 cagttgagcg tggtcgaggc cgtatgcgga ttttccgcca cttgcctgaa agaatcgctg
     4141 gtggccatgc aaacttcgct ggtggccctt tctgtgaaga ccaggctttc ctggtcgatg
     4201 tggaagtcgc tcacgaagag tgattcgcct ggttcaggtt catccctatc gtggatggtt
     4261 gtatcaatac ttatatcttt ctgcttctcc tgggagactc cgaacgattg cagaccttta
     4321 tcctggctga gggaaggcag gctggagggc ggcagagccg ttatttcacc ggcgcccggc
     4381 ctggcgaagc gggagaagga gcgactcatc ttttcgcttt cggccaggct gtccggctcc
     4441 gaaggcgaaa atcgttcggt ttcctgggga actgcatttg ctacatatat aggagcggca
     4501 ctggcctggg aggcgtcttt gagagcgcca tggggtgcgg caggagcctg cacttcctcc
     4561 gagctttgac ctgcaaccgg ctcctgtgtg ccgttgcttt caagccggtc gactaccttt
     4621 ctaaggagtc gggccagttt gttttgaaag gtttccatcc cccgagtaac cccacttacg
     4681 cccaagtaag aaaatagaga aatataaatc gatgctatcg cgccactcgt gggcatgggt
     4741 atagatttgg ccgtatttct atagggggca acctatacag ttgtcaggtg cctatttttg
     4801 cctgtatttc acgtttttgg cgagtttata cgattcccgt acaaagttat aggattgggg
     4861 agcagcgccg gcatcctgca ggggtaagaa tgctaatgag ccccctgttt gggaccgttc
     4921 accaggcagt ggtcaatttg atggaaggct cttgattcat taccatcctt ctaaagaagg
     4981 tacgaaattc ggaaatggta tcaagaagcc tggcgaagca ggacagtgta acttcgcttc
     5041 aggcaggccg acattcacaa aggcagagag cacgagaacc gaggaagcta tgaagtgtga
     5101 aactattccg gaacttgaga gattcttgaa cattcaggag caggcgctcg gatgcaacag
     5161 ccctgaagta gccaacacca ttaccaggtt ggcggaactc tactttagta agggtttgct
     5221 tgaagatgct gaagcccttt atcgtcgcgc tctcaatata agggagcaag cgcttggtcc
     5281 tcaccgggta gaaattgaag ataccaaaaa gaatctagag aaggtattaa cccgtaagcg
     5341 tgctccctct cagagccgca caccccagag tatgaccgat tcttctacat cgctggatgc
     5401 tgttccggcc tctatggccc ttggttggcc ttctgctttc gatcaagata aagagaagga
     5461 atttgaggtg gaactggctt ctcttcggca catgatgggg cgcgagcatc cgcaggtggc
     5521 agactgcttg actcgtatgg cagatcaata ttgccggaac aagcgctatc gggagatgga
     5581 acccctcttg caggaagcgc tgaagataag ggag
//
LOCUS       DAOQNZ010000042         5601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5793_length_5601_cov_3.672557, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000042 DAOQNZ010000000
VERSION     DAOQNZ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5793_length_5601_cov_3.672557"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKW73_01350"
     CDS             <1..426
                     /locus_tag="PKW73_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA71953.1"
                     /translation="MLKLHQHEPQILHRDLTPDNLVITNNGNLALIDFGSAHQFVEGI
                     TGTLVGKQAYIAPEQLRGKATIQSDIYSFGCCLYFLATGKEPQALKECDAIASGASIT
                     NSLNELIRCCTSFEAKERPENFQEVLARLGNGNNREGSE"
     gene            423..2690
                     /locus_tag="PKW73_01355"
     CDS             423..2690
                     /locus_tag="PKW73_01355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA71954.1"
                     /translation="MNRLRLTYAESHLPMQELSGLPQKVLELRAGLTHLNKVDKLGAE
                     LSIVLNTTYYVLCLLFFHLFRGFYRNGTDGPEWSGLKNLENDKEQYLRKTFEAQEPGF
                     GKSFLLNSAPVAGLLLVTLVSLKAGLVIVLTIFIAWSLYGLFRAIAPAALRKRIDESK
                     LAGYAHTPLAESTSTALKHLRPFFNIGIFLSVYGGVVMMLFHWLARLTGLHERLQNYR
                     EEKPGSLVLAQNLPAISEREENFFDSPVFGLTILTVLMSGIRSYLTYLLYLKSGVDAF
                     FGYPSLDPQFKEIMFILLYFGTVGCALSVLFFKGWFTFPLNFQSSEYDVVLNDKKISK
                     EPAKGWFGDLMCWGFDPVYFTRELAWQDITKIDYGPGVSFRLYPLPTTHLSAQSDLYK
                     LLNKLAALCDSFGAARVKDHTLTITSLRGPTLSIRLFELNENQKVELFKFVCKHAPHA
                     EITARAQEALVGSNLLKDPRHTELWFDMLTEYTRVVQAGGCESAESAASIGTGSTLKG
                     NSLTVERLLGGGGQATVYLARNESGEKVVLKEFVLTAGEGVEALVESAADFEAESTIL
                     SRLDHEQIVKLKDLFIENNRAYLVIEYIQGRSLRSLVGEDGALPEDKVRELALQMCDI
                     LTYMESQTPPVVHRDFTPDNLILQSDGRLKLIDFSISQLFQAATTAECAGKHAYTPPE
                     QYRGQASPQSDIYAMGATLFFLLTRQDPEPISSSHPAALNAAISSGLDAIVAKATAIN
                     LSDRYESAAWVRMDV"
     gene            2789..4462
                     /locus_tag="PKW73_01360"
     CDS             2789..4462
                     /locus_tag="PKW73_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNA71955.1"
                     /translation="MKKLLFTASLLLLALGGNDSLLQANIPSLLPTARAAEQEESSLE
                     KKADRQPLFIWKCKKEDQTVYLLGTIHVAKANFYPLPAEMENALNESRILFVEADVLQ
                     ADPNKTMDIMKKRGICQPPATLTQSLSPETVEIFKQYLDWAGENLSAYQPYKPWVVSQ
                     LVANAALRKAGYKPELGIDRHLLNEAHAVGKKIIPLESIDMQLSLMDSFDASTQEKLL
                     LGTLITNKDTRANLETLEKNWKEGKVDELTSDKDKTGSSEDLEKVRVAMLDKRNQTMV
                     ENILKNLPPKDTAMVAVGAAHLGGQGGLLAKLKEHGFEVEQITAALKKPTTAISFGGS
                     ALKRLYFSEGLFRIMLPGDPEVKYEDMNGIRMVDYGYNHFNGAMTVSYLIMPRAIPQI
                     DKQKQFMQLIANAVTAKLKGTGGSLQPLSNAPVTMLYSCKLPGNDKLTGKEIYLREKM
                     LLSGRRLYLVGGQGTTDFLTSKLYGQFEGSLEVIPEQGYATAASFGQPGSTGFGSSHT
                     AANRSASSFNHADFDKTRKEMQQRSDEFRRKVQKDLEKSRADFQFGRTR"
     gene            complement(4494..5213)
                     /locus_tag="PKW73_01365"
     CDS             complement(4494..5213)
                     /locus_tag="PKW73_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71956.1"
                     /translation="MPASSACKKVMTMMLRIPNTAFYLALLVLLLVVGLVAPVVAIAR
                     PMKAGFLINADFAAYVLIAFTFMLKGLALRRSFVAVPLTKDLILKALGLYLYLSLFLM
                     SSLLCRRLKLAMLPDQYYWLSYAAIVPLTFSLLLILKGRNSGLASVVSYPVYLGLLMS
                     MAFFPLVQMCWFPLLALPGVLIVMAWRIDNAERNSLERAFELEAPAADGTLIELVEDD
                     REKLAPLEVIEPRFKIIPFLY"
     gene            complement(5200..>5601)
                     /locus_tag="PKW73_01370"
     CDS             complement(5200..>5601)
                     /locus_tag="PKW73_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNA71957.1"
                     /translation="QSVTGIGPRLALALVGTLGVDTLVEAVLTENQKMISQAPGVGAK
                     VAQRIILELKSKIEDFAGTRTANRSESPTGARTARAVVEEVTEILSHYGYTPTEIHSA
                     LKQAESEQVARDTAEELLRFTLKILGAGARK"
BASE COUNT         1509 a         1502 c         1399 g         1191 t
ORIGIN      
        1 atgctgaaac tgcaccagca cgaaccccaa atattgcacc gcgatctgac accggacaac
       61 ctggtcataa ccaataacgg caacctggcc ctcattgatt ttggttcggc ccatcagttt
      121 gtcgagggca ttaccggtac tctcgtgggc aaacaggctt atatagcgcc ggaacaactg
      181 aggggcaaag ccaccattca aagcgacatc tacagtttcg gatgctgcct ctattttctt
      241 gccaccggca aagagccgca ggcactgaaa gaatgcgatg ccattgcaag tggtgcatct
      301 ataacaaact cacttaacga actaattcga tgctgcacaa gttttgaggc aaaagaacga
      361 ccggagaact tccaagaggt tctcgcgaga ctgggtaatg gaaacaaccg ggaaggcagt
      421 gaatgaacag gctgaggctc acgtatgcag aaagtcatct gcccatgcaa gaactgagtg
      481 gacttccaca gaaggttctt gaactgcggg ccggacttac tcatctaaac aaagtcgaca
      541 aacttggtgc tgaactcagt atagttctaa acacgactta ctatgttctc tgccttctct
      601 tcttccacct cttcagaggg ttctatcgaa acggcacaga cggaccggag tggagcggtc
      661 tcaagaacct tgaaaacgat aaagagcagt acctcagaaa gactttcgaa gcccaggaac
      721 cagggttcgg caagagcttc ctgctcaatt cagcccctgt agcagggctc cttctggtca
      781 ctctcgtctc tctcaaagct ggtctggtca tcgttctcac catttttata gcatggtcac
      841 tgtacggtct cttcagagct attgcaccgg cggccctgcg caaacgcata gacgaaagca
      901 agctggccgg gtatgcccat acgccgcttg cagaaagcac tagcaccgct ctcaagcacc
      961 tgcgtccttt cttcaatatt ggcatttttc tctcagtcta tgggggcgta gtcatgatgc
     1021 tctttcactg gctggctcgc ctcaccggct tgcacgaaag actgcaaaac tacagggaag
     1081 aaaaaccggg cagcctggtg ttggcacaaa atctaccggc catatctgaa agagaagaga
     1141 acttcttcga ctcaccggtc ttcggactga ccattctcac cgtcctgatg agcggcattc
     1201 gcagctacct gacttatctg ctctacttaa agagcggcgt agacgccttt ttcggctatc
     1261 cgtctcttga ccctcagttc aaggagataa tgtttattct gctttatttc ggtacggttg
     1321 gctgtgccct cagtgtgctt tttttcaaag gctggtttac atttcccctc aattttcaaa
     1381 gcagcgaata cgacgttgtg ctgaacgaca aaaagatcag taaggaacca gccaaaggct
     1441 ggtttggcga cctgatgtgt tgggggtttg atccggttta cttcacaaga gagctagcct
     1501 ggcaagatat caccaaaatc gactatggac caggcgtcag cttcagactt tatcccctgc
     1561 ccaccacgca tctgagcgcc cagagcgacc tttacaagtt gctcaataaa ctggcggcgc
     1621 tctgcgactc tttcggcgcc gccagagtga aagaccatac tttgacaata acctccctca
     1681 gagggccgac actttcaatc agacttttcg agctaaatga aaatcagaaa gtagagcttt
     1741 ttaaatttgt atgcaagcac gctccccacg ctgaaatcac cgctcgggca caggaagccc
     1801 tggtaggttc caacttgctg aaagacccgc ggcacaccga actctggttc gacatgctga
     1861 ccgaatacac cagagttgtg caggcgggcg ggtgcgagtc tgctgagagt gccgcgtcta
     1921 tcggtacggg cagcaccctc aagggcaatt cactaacggt agagcgcctt cttggcggcg
     1981 gcggacaggc cactgtctac ctggccagaa atgaaagcgg tgaaaaggtg gtgcttaaag
     2041 aattcgtctt gactgccggc gaaggcgtag aggccctggt tgaatcggcg gcggatttcg
     2101 aagcggagag tacgattttg agcaggctcg accacgagca aatcgtcaaa ctaaaagact
     2161 tatttattga aaacaaccgc gcctatctgg tcatcgaata cattcagggg cgaagcttgc
     2221 gcagtctggt cggagaagat ggcgccctgc cggaagataa agtaagggag ctagccctgc
     2281 aaatgtgcga cattctcact tatatggaaa gtcaaacacc accagtggtg caccgtgatt
     2341 tcactccgga caatcttatt ttgcaaagtg acggacgcct caaactcata gatttcagta
     2401 tttcgcaact tttccaagcc gctaccacgg cagaatgcgc cggcaagcat gcttatacac
     2461 cgccggagca gtaccgcggt caggcctcac cccagtcgga catttacgcc atgggagcca
     2521 ccctcttctt cctattgacg cgacaagacc cggaacccat aagcagttcc catccagccg
     2581 cccttaatgc cgccatttcc agtggacttg acgccattgt ggcaaaagcc acggccatta
     2641 atctcagcga ccgctacgaa agtgccgcct gggtgaggat ggatgtataa aagtacgaat
     2701 ataagtgcaa ttaaccagtc agggcgattg gactgccaga gcaacgggta gaacctttgg
     2761 agattccagc cgaggaccag ttgcgaagat gaagaagcta cttttcacgg cgtccctgct
     2821 tttgcttgcc ctgggcggca atgacagtct gctccaggcg aacatcccgt ctttgctgcc
     2881 gaccgcccgg gccgccgagc aagaagagag cagtctcgaa aaaaaagccg acaggcagcc
     2941 cctattcatc tggaaatgca agaaagaaga ccagacggtc tatcttctgg gcaccataca
     3001 tgtggccaaa gcaaatttct atccactgcc ggcggagatg gaaaatgccc tcaatgagag
     3061 ccgcattctc tttgtagagg ccgatgtatt gcaggcagat ccaaataaaa ctatggacat
     3121 catgaaaaag cgcgggatct gccaaccgcc tgccaccttg actcaatcac tatcgcctga
     3181 aacggtagag atcttcaaac aataccttga ctgggccgga gaaaacctca gcgcttacca
     3241 gccctacaaa ccatgggtgg tatcacagct tgtggccaat gccgccctgc gcaaagccgg
     3301 atacaaacca gagcttggca tagacagaca cttgctcaat gaggcacacg cagtcggcaa
     3361 aaagatcatt cctctggaat ctatcgacat gcaattgagc ctcatggaca gcttcgatgc
     3421 ctccacacaa gagaagttgt tgctgggaac gctcatcacg aacaaagata cccgagccaa
     3481 tctggagacc cttgagaaga actggaaaga gggcaaagtc gatgaattaa ctagtgacaa
     3541 agacaaaaca ggcagttcgg aggatctgga gaaagtaaga gtggccatgc tggataagcg
     3601 aaaccagacc atggtggaaa atattctcaa gaacctgccg cctaaagaca ccgcaatggt
     3661 ggctgtgggg gcggcacacc tgggcggtca aggcggtctt ctggctaaat tgaaagaaca
     3721 tggttttgaa gttgaacaga tcactgccgc cctgaaaaaa ccaacaaccg ccataagttt
     3781 cggcggcagc gccctgaagc gcctctactt ttcagaaggg ctcttccgca ttatgctgcc
     3841 cggcgacccg gaagtaaaat atgaagacat gaacggcatt cgcatggttg actacggcta
     3901 caaccacttc aacggtgcca tgacagtatc gtatctaata atgcccaggg ctattcccca
     3961 gatagataaa caaaagcagt tcatgcaact tattgcaaac gccgtcaccg ccaaactgaa
     4021 gggcacgggc ggcagcctgc agccgctatc gaacgcgcct gtgactatgc tgtacagttg
     4081 caaacttccc ggcaacgaca aactcaccgg caaagagatc tatttaagag aaaaaatgct
     4141 cttgagcggg agacgcctct accttgtggg cgggcagggc accacggatt ttctaacatc
     4201 gaagttgtac gggcagtttg aaggttcact cgaggtcatt ccagagcaag gttatgccac
     4261 ggctgccagc ttcggtcagc cgggttcgac cggctttggt agcagccata cagccgccaa
     4321 ccgatcagcc tctagcttca atcacgctga ctttgacaaa acccgcaaag aaatgcaaca
     4381 gcgatcggat gagttcaggc ggaaagtaca aaaggatctg gaaaagtcta gagcagactt
     4441 tcaattcgga agaactcgct agcccaacca accaacttaa ccctctagag acttcaatac
     4501 aaaaacggaa ttatcttaaa gcgcggctcg attacttcaa gaggagccag cttctctctg
     4561 tcgtcttcca caagttctat caaagtgcca tcggcagccg gtgcctccaa ctcaaaggct
     4621 cgctccaagc tatttcgctc ggcattatct atgcgccagg ccattactat tagcaccccg
     4681 ggcagtgcca atagcggaaa ccaacacatc tgcaccagcg gaaagaaagc catggacatt
     4741 aagagaccca ggtatacggg ataggaaacc actgaagcaa gaccggaatt gcgtcctttt
     4801 agaatcaaaa gcagggagaa ggtgagcggc acgatggctg catagctcag ccagtagtac
     4861 tgatcgggaa gcatggccag cttcaatcgg cggcacagaa gcgaactcat gagaaagaga
     4921 ctgagataga ggtacagccc taaagccttc agtatgagat ctttcgtgag aggcacagcc
     4981 acaaagctcc gacgcagggc aagccccttc agcatgaagg tgaaagcgat gagcacatag
     5041 gcagcaaaat cggcgttgat taaaaacccg gctttcatgg gtctggcaat cgccaccacc
     5101 ggcgctacca gccccacaac cagtaaaagc accagcagag ccagataaaa tgccgtattc
     5161 ggtatgcgaa gcatcatggt catgaccttc ttgcaagcgc tacttgcggg caccggctcc
     5221 cagaattttg agagtaaagc gcaaaagctc ttcggcagtg tcccgtgcta cctgttcgct
     5281 ttctgcttgt ttgagggcac tgtgaatttc ggtgggtgta tatccgtaat gggagagaat
     5341 ctcggtgact tcctccacta ccgcccgagc ggttcgggct ccggtagggc tttcgctgcg
     5401 gttagcagtg cgggtaccgg caaaatcttc tattttgctc ttcaattcta gaatgatacg
     5461 ctgcgctacc ttggcaccta cgcccggagc ctgcgaaatc atcttctggt tttcagtcaa
     5521 gacagcctct accagggtat ccacccccag ggtacctacg agagccaggg ccaggcgcgg
     5581 gcctatgccg gtaaccgact g
//
LOCUS       DAOQNZ010000043         5579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5823_length_5579_cov_3.904960, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000043 DAOQNZ010000000
VERSION     DAOQNZ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5823_length_5579_cov_3.904960"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKW73_01375"
     CDS             complement(<1..816)
                     /locus_tag="PKW73_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA71958.1"
                     /translation="MTNDVVLTDIFGTPFSLVDKRQAVSENTDKIFIIKPYLQLGRHF
                     NEKPGSLVLFFHPGKKYRGKDWLVQYRQPSVDGDNWRDASLSRGRLRLISGKRHWRYR
                     AELTGLSAGEPFQYRVVKAWTLVFSGEAQAPSAEKARIAVLGDMADGGQASADVARAM
                     LASRPELLLAVGDIVYDKGLVSEYLAKFFPVYNETAGSCSVPLLSSLPLAPVIGNHDI
                     GTPGQQEKPDFDHADALGCCKFFENPRGNLGLKRKQLKKGLSSRRAAFIDSLPR"
     gene            complement(1655..2251)
                     /locus_tag="PKW73_01380"
     CDS             complement(1655..2251)
                     /locus_tag="PKW73_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71959.1"
                     /translation="MSTSLAFGRKLALSVSEQAVDALRTLILQRDAIFARLEVLRNRA
                     VPSETESEDGGEEHGPSCLDPLCAQTDAAHNAGSLILDGVAADADADDKPLSLEEQVC
                     LSMGQTLQVFAGTHIDAALSCLAHNPRAFEAIKAEIEPDMVQHWLDSAADDVAYGPLM
                     GFSPEAVTTGDMLMVGEHVRAAQALHERILQLLKEAGA"
     gene            complement(2311..3735)
                     /locus_tag="PKW73_01385"
     CDS             complement(2311..3735)
                     /locus_tag="PKW73_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNA71960.1"
                     /translation="MKLKQLLSLLTASICSCRSRVSKASGARFSAFRLDKLPLPKLRA
                     SSSGAQSAPSVSGKSAPQAHSGGALAAKGLALLDLSSGAPDSDTPDVLKQAAAQAIMN
                     GDNQYGESNGDLELRRLYARRLTEQMTLTRNLAGVSSPAALADAACAIDPDTQVTVLN
                     GTSSALAAVLLSTVDAGDEVLVFAPYFEGYLQAIAMAEARAVPVPLGNPPSTGFAAFP
                     LAPAGFNWSIDEAALRAAITPATKAIIINSPHNPTGRVFTAAELSIIAAVAREHDLLV
                     IADEIYNLLTYQGEGAEAPVHLSIRALPGMAERSVVIDGLSKTHSASGWRIGFVTAPV
                     TITRQLRRIAAALGLSAPTPLVVASRLAYLPASDPHGRALADVLSANRQELERNGRVL
                     ANALRRQGFALAQPEGATYILAGLPGRSGFDDLAQFLHDRCLIKVAPGTAFFDGDEGR
                     LWVRFCFARRPEIIDQAVERLALI"
     gene            4043..5437
                     /locus_tag="PKW73_01390"
     CDS             4043..5437
                     /locus_tag="PKW73_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNA71961.1"
                     /translation="MTYEEALKFIQSFPDMERATFGARGPTMGLPAMKSLLTRLGDPH
                     KGTKTIHIAGSKGKGSTSTFVAAMLAEAGTQTALYTSPHLHDYPERFAFNMEAISEET
                     FARGLSEIKGRIEEERDAGNTSISTFGVLTALFFHLVKTASKPVDWQVVEVGLGGRYD
                     VTNVFESKDVAIITPISLEHVEILGSTQTEIAANKAGIIVPHCVSILAPQKDAGARTA
                     VGRRCHEVDAELIDVGKKFKLKAISQDISGQTFTMEGLQTSMELSISMLGSHQMGNAA
                     TAVAAILAMRSKGKLDISDEQIRAGLKKASIKGRLEVLRKGAAFGGSGPTIVADGAHN
                     HESAQALSQALQHFFKAKQCVFVIGVNNDKNIGAIWKELSHLNKAAIATRSDNPRSMN
                     PEEIIDMMSVFEAERPELHTSQNVPEAIEKALAIADDDDVICVTGSLYVVAEARAYLL
                     AKEQAGKTAARFSI"
BASE COUNT         1391 a         1549 c         1562 g         1077 t
ORIGIN      
        1 ccggggcaga ctgtcaatga aggccgcccg gcgactggaa agacccttct tcaactgctt
       61 gcgcttcaga ccaagattgc cgcgcggatt ctcgaagaac ttgcagcagc cgagggcatc
      121 ggcatgatca aaatcaggct tctcctgctg tccgggcgtg cctatatcgt gattgccgat
      181 cacaggcgcc agaggcagac tggatagaag cggcacggaa caactgccgg cagtctcatt
      241 gtagacaggg aaaaacttag ccaggtactc actgaccaga cccttgtcat agacaatgtc
      301 gcccacggcc agaagcaatt ccggacggct ggccagcatg gctcttgcca catctgcact
      361 ggcctgacca ccatcagcca tatcaccaag aacggcaata cgagccttct cagcagacgg
      421 cgcctgagct tcaccggaga ataccagagt ccaggccttt accacccgat actggaaagg
      481 ctccccggca gaaagtccgg tcagttcagc ccggtagcgc caatggcgtt tgccggagat
      541 aagacgcagg cgcccccgag aaagagaagc atcccgccag ttgtcgccat ccaccgaggg
      601 ttgacgatac tgcacaagcc agtctttacc acggtatttc ttaccgggat gaaagaagag
      661 cacgagagag ccgggcttct cattgaagtg ccgacctaat tgcagataag gtttgatgat
      721 aaagattttg tccgtatttt cggaaaccgc ctggcgcttg tccacaagcg agaaaggcgt
      781 gccgaagata tcggtgagaa cgacgtcgtt agtcatgact cttcacctct ggtttggctt
      841 ttgccgattg ataggttgtt tgagcaagca agctccccat ggaatccccc attgaagact
      901 gtcaaacacc tgtcagcacc tgcactgctt gttataagca gtcatgatgt tcaggcaatt
      961 cttcgggatt ctggtaagcg ggttgaaagg ggattctgag agtaggattt aaaaatgagc
     1021 ttgcaccaaa atctaagctc cttcagccct caaagtcatg catcgatact ttgcttcttt
     1081 cgctgaatgg cccgaaaatg ggacccgcag cctgccaaga gagaaagaat agtaatttct
     1141 ttctcggcaa tcacttcgcg ccgctagcgc atgccaccac cggcggcgct agcagagatc
     1201 gggttaagaa caggttatga agcaaagtcc aaagaagcgc catagacata ttcagaccaa
     1261 gacaaggatt cgatctatct gagcccgcca atattgctcc aggctaattc tcaaactctc
     1321 aaccagcaag caattatgac ctggcacaag accagtgcca cagcccattc aagcgagctg
     1381 ccaaagacgt agttgccgat ttgcgtcact tccataatgc cgtttcacca cccggttaca
     1441 tgtacttact ttagtgaggc aaatagtcaa gattcgggca gactgcttaa acaaaacaat
     1501 gactggattc tgtgcctagg tccgaaccta tggccgaatc ctacttcgcg ctgtagtata
     1561 ttcaaaacaa aagcaaacgc ggggaaacag ggaagattgg catcctccct gttttatccc
     1621 cgcgctgcct agggctcaga gcccggcacg ttcatcacgc gccagcctct ttgagcaact
     1681 gcaggatgcg ctcgtgcaga gcctgagccg cccgcacgtg ttctcccacc atgagcatgt
     1741 cgccggtggt caccgcttcg gggctgaagc ccatgagcgg accataggca acatcatcgg
     1801 cggccgaatc cagccagtgc tgcaccatgt ccggctcaat ctcagccttg atggcttcga
     1861 aggcgcgcgg gttgtgagcc agacaggaga gcgccgcatc gatgtgggtg cctgcgaaaa
     1921 cctgcagggt ttgccccatg gaaagacaga cctgctcctc gagggagagg ggcttgtcgt
     1981 cggcgtcggc gtctgcggcg acaccgtcca gaatgagcga accggcattg tgcgctgcgt
     2041 ccgtttgcgc acacagaggg tcgaggcagg aaggaccatg ctcttctcca ccatcctccg
     2101 actcggtctc cgaaggcacg gcgcgattgc gcagcacctc caggcgagcg aagatggcgt
     2161 cacgctgcaa gatgagcgtg cgaagagcgt cgacagcctg ctccgaaaca gaaagggcga
     2221 gctttcgccc gaaagccaga gatgtggaca taagcactcc ttatgagatt gtgagattgt
     2281 gaggttgatc tagttgatcg acgaacctag ttagatgagc gcgagtcgct ccaccgcctg
     2341 gtcgatgatc tcgggccggc gagcgaagca gaaacgcacc cagagtcgcc cctcgtctcc
     2401 gtcaaagaag gcggtgccgg gagcaacctt gatcaggcag cggtcgtgca aaaactgagc
     2461 gaggtcatcg aacccgcttc tgcctggcag accggccaga atgtaggtag cgccttcagg
     2521 ctgagccaga gcaaagccct gccgcctcaa ggcgtttgcc agcactcgac cgttccgctc
     2581 cagttcttgc cgattggccg atagaacatc tgccagagcc cggccatgcg gatcggaagc
     2641 aggcagataa gccaagcgac tcgccaccac cagcggtgta ggggcggaga gtccgagggc
     2701 ggcggcgatc cgacgcaact gccgggtaat agtcaccggg gcggtcacga agccgatacg
     2761 ccagccgctg gcactgtggg tcttggaaag accatcgatg acgacagagc gctcagccat
     2821 gcccggtaga gcgcgaatgc tcaagtggac aggcgcttcc gccccttcac cctggtaggt
     2881 gagcaggttg tagatctcat cggcgatgac gagcaggtcg tgctcccggg caacggcggc
     2941 gatgatggac agttccgcag cggtgaaaac ccgaccggtg ggattgtggg gactgttgat
     3001 gatgatggcc ttggtggccg gcgtgatggc cgcgcgcagg gccgcctcat caatggacca
     3061 gttgaaaccg gcgggagcaa gcgggaaagc cgcgaaaccc gtcgaaggcg gattaccgag
     3121 agggacaggc acggcccggg cttccgccat ggcgatggcc tgcaggtaac cttcgaaata
     3181 gggcgcgaaa accagcacct catcaccggc gtccactgtg gagagaagca ccgccgccag
     3241 agccgaggaa gtgccgttca agacggtcac ttgcgtgtcc gggtcaatgg cacaggcggc
     3301 atctgccagg gcggcgggcg aagacacacc ggccaggttg cgggtcaggg tcatctgctc
     3361 agtcagccgc cgggcgtaga ggcgtctcaa ctccaggtcg ccgttgcttt cgccgtactg
     3421 gttgtcacca ttcatgatgg cttgcgcggc cgcttgcttg agaacatcgg gggtatcgct
     3481 gtcaggagca ccactggaga ggtccagaag ggcaagcccc ttggcagcga gagccccgcc
     3541 cgagtgagcc tgcggtgcag actttcccga gaccgatgga gccgactgag cgccggaaga
     3601 gctagcccgg agcttgggca ggggcagctt atccaggcgg aaggccgaga agcgcgcgcc
     3661 ggaggcttta gatactcgtg agcgacaact acagatagaa gctgtcaaca aggacagcag
     3721 ttgtttcaat ttcatagcca gcacctcaat cgtggaactt taagccaacc ttcaggacca
     3781 cccaaaagcg aaggcaaaaa aatttccacg aaatctgttt agggggttaa atcacacgag
     3841 cacggtcttc cagcgatgac aggcacttgc ctttcacctc ttcaaaactt gtaatggaag
     3901 accagatagt gtttagttac ttagagacaa actatgtcga gagaaaatac tcaaatttca
     3961 gcttgaaacc taagctatta agcaagaagt tttcaagata aagagatgaa aggtatttca
     4021 agtaccaagt taaagttagg cgatgacgta cgaggaagct ttgaaattca ttcagtcctt
     4081 tcccgacatg gaaagggcta cttttggcgc tagaggtcca accatggggc ttcctgccat
     4141 gaagtccctc ttgacgcgcc tgggtgaccc ccacaaaggt accaagacca ttcacattgc
     4201 cggctccaag ggcaaaggct ccaccagcac tttcgtggcg gccatgctgg cggaagccgg
     4261 tacgcaaact gcgctatata ccagtccgca cctgcatgat tatccggagc gcttcgcttt
     4321 caacatggaa gccatctccg aagagacttt tgccagaggt ctgagcgaga tcaaagggcg
     4381 catagaagaa gagcgagatg ccgggaacac cagcatttcc accttcggcg tcttgacagc
     4441 cctatttttc cacctggtca aaaccgctag caaaccagtc gactggcagg tggtggaagt
     4501 tggactgggt ggacgttacg acgtcaccaa tgtattcgaa tccaaagatg tggccattat
     4561 cacccccata agcctcgagc atgtggagat attgggcagc acccagaccg agattgccgc
     4621 caataaggcc ggcataatcg tgccccactg tgtttcaatt ctcgcccccc agaaagatgc
     4681 cggggctcgc acggctgtag gtaggcgctg ccacgaagta gacgccgaac tgatagacgt
     4741 aggcaagaaa ttcaaactga aagccattag tcaggatata tccgggcaga ccttcaccat
     4801 ggaaggtttg cagacctcta tggaactctc catcagcatg ctgggcagtc accagatggg
     4861 taacgctgca acggctgtag cagccattct ggccatgaga agcaaaggta aactggatat
     4921 cagcgacgaa cagatccgag ccggattaaa gaaagcctcg atcaaaggcc gcctggaagt
     4981 attgcgcaaa ggtgcagcct ttggcggttc cggccccaca atcgtagcgg acggcgcgca
     5041 caaccacgaa tcggcccagg ccttaagcca ggcgctgcaa cactttttca aagccaagca
     5101 gtgcgttttc gtaataggcg tcaacaacga taaaaacatc ggtgccatct ggaaagagct
     5161 atctcacctg aacaaagcgg ctatagccac acgctccgac aatccacgct cgatgaaccc
     5221 cgaagaaatc atcgatatga tgagcgtttt cgaagcggaa agacctgaat tgcatacttc
     5281 tcagaacgtg ccggaagcaa tcgaaaaagc tctggccatc gccgatgacg acgacgtcat
     5341 ttgcgtcacg ggttcgctct atgtagttgc cgaagccaga gcttatttac tagccaaaga
     5401 acaggccggc aaaaccgccg cccgtttcag catctaaacc aaagcgcctg agaggctagc
     5461 gtgcctttcc cttagcggca agagcgcgct cgataaaggc tttgatttgt tctttgtctt
     5521 tgaacccggt catggtggcg gggctcttag tgccgggcat aaagacaaac aaaaccggc
//
LOCUS       DAOQNZ010000044         5558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5862_length_5558_cov_3.934581, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000044 DAOQNZ010000000
VERSION     DAOQNZ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5862_length_5558_cov_3.934581"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1579)
                     /locus_tag="PKW73_01395"
     CDS             complement(119..1579)
                     /locus_tag="PKW73_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HNA71962.1"
                     /translation="MIPKLLIALALAGLIFFLVGRKQKVQAAPENRPRLTNGGKEKAT
                     AKTLAVVRTSPETRMQESTLSFQVSQSQGSMQAAVPTITVLTETKQRIVGFGGAFTDA
                     ACFMFNEMPKDKRAALFQELFGHGGLNLNFCRTCIGASDYATHVYSYDEGEPDPELKR
                     FSIEHDRKYILPIIREALAVNPDIFLYGSAWSPPGWMKSNGSMLGGAMRRHSLEPYSR
                     YLLKFVQAYGEEGVKIKAVTVQNEVDTDQDGNMPACIWAQELEVDFVRGFLGPLFQKE
                     GIDTKIWIIDHNYNLWGRALGSLDTPGMLKYTDGIAWHGYVGDASKVSTVHDSYPDVS
                     MFWTEGGPDYTNADYGTDWCKWSRTFSNILSNWCRGITVWNLALNEVGKPNIGPFPCG
                     GLVTINSQTKEVSLSGQYFALAHFSKYVKPGARVLCTSQSGQENSLSRVAFVNPDGGK
                     VLVVTNSGAATAVDLVEGGNQVHLDLPADSVTTLRW"
     gene            complement(1676..2080)
                     /locus_tag="PKW73_01400"
     CDS             complement(1676..2080)
                     /locus_tag="PKW73_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71963.1"
                     /translation="MTAFIITLTIGAVVFLIMFLAFRRSSKRRETGRNTYKSSSGSSH
                     SGSRLSNVKDNNRRDSGSSDDSSIGLWPFLGSSGDGNTHKNSVTDHGTSTTDTSHSSG
                     HSSCSSGHSSCGGGSSCGGGSSCGGGGGCGGS"
     gene            complement(2243..2578)
                     /locus_tag="PKW73_01405"
     CDS             complement(2243..2578)
                     /locus_tag="PKW73_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71964.1"
                     /translation="MTEKTTNQIQDVLDGKFDTLKALLRKVETSKDVIAAFDAGYYTG
                     LYRAVLPIEELTRNTCQIAKNLDEGNALSRERAREVSQQYLLVVDCRGPVAEIVLELL
                     DLTAEDSTD"
     gene            complement(2694..2912)
                     /locus_tag="PKW73_01410"
     CDS             complement(2694..2912)
                     /locus_tag="PKW73_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71965.1"
                     /translation="MHIDPVQLILLIIVFYVVLQVIRRTTQTMANFVIFILVLAVLMP
                     NETRGLLSGLTRLFMEIVHRLAYVLGWS"
     gene            complement(2938..3645)
                     /locus_tag="PKW73_01415"
     CDS             complement(2938..3645)
                     /locus_tag="PKW73_01415"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNA71966.1"
                     /translation="MNFSQVKSHILRGAGLFFPFAAILWGFNLALKIADGWFGSLITF
                     IWQEFWPNTVSPAHSPIFSLVFLLVMLYTISVVVSWKLGSYIFNWVEGRIYSIKGIGA
                     IYSSLRKGIDMVSKSGAQESFKRVVWVPFTSGGGVTIAFVTKEIINRTNGKRYVVVMI
                     PTPPNPISGLMVAYPEDRIADCDMSIDAALQMCMSLGIAAPESINLTPAAEAVAAFVS
                     QTASEHGAVVLTAESQV"
     gene            4344..4904
                     /locus_tag="PKW73_01420"
     CDS             4344..4904
                     /locus_tag="PKW73_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71967.1"
                     /translation="MSYNPENSKLTGLIRQAEDRLQSVEERLVNLLIEATYGKVGLLH
                     INLDGPQLLGRKCFDRIFYGRRLPSLRELTRLAERLNMELEVLVLCRRSQATVRSFKA
                     VRGFSRAQLREFLLAVQASASPGQDTVISRRCRSMIDWLGLGRARIYWRNLAIIAALC
                     GYAIDIKLVPKDRGNQALLQRLDYPL"
     gene            5074..>5558
                     /locus_tag="PKW73_01425"
     CDS             5074..>5558
                     /locus_tag="PKW73_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71968.1"
                     /translation="MKPTVKLDQKALTRHKKRFLRFFPNSFQDQQYIDWERTYKWEAH
                     KLFQEHLARDKFKTLMNKGQYDEIAMRALQVEARTNFIFSFEKMALRDGVKAPGGART
                     FAEGLYGLLYERGPLSGRFIQWLVALADLPRKKSRVLSWPVATFFPFIAQPGKHIILK
                     PT"
BASE COUNT         1181 a         1355 c         1582 g         1440 t
ORIGIN      
        1 gcggctcgct gtggcggagt tatggcggcg aaagcactca aaacaggccg ctttcgcttg
       61 atggaattga gacgcttgtt cggctgtccc ttccctggga ctcgcctacg aatacttgct
      121 accagcgcag tgttgtcacg gagtcagccg gtagatcaag gtgaacttga ttgccgcctt
      181 ccaccaggtc gactgccgtg gctgcaccac tattggtgac taccagcact ttgccgccat
      241 cgggattaac gaaggcaacg cgagagagtg agttctcctg accggattga ctggtgcaca
      301 gtaccctcgc tcccggtttc acatacttgg agaaatgagc aagagcgaag tactgtccgc
      361 tcaggcttac ctctttggtt tgagagttga tagttaccag cccgccgcaa ggaaaaggcc
      421 cgatattggg cttgcctact tcgtttaggg ccagattcca gacggtgata ccgcggcacc
      481 agttggagag gatgttggag aaggtgcgcg accatttgca ccagtctgtt ccgtagtcgg
      541 cattggtgta gtccggccct ccttccgtcc agaacatgct cacgtcagga tagctgtcgt
      601 gtaccgtgct tactttggaa gcgtcgccca catagccatg ccaggctatg ccgtccgtgt
      661 atttgagcat gcccggtgta tccagggaac ccagggcgcg tccccagaga ttgtagttgt
      721 ggtcgatgat ccagatcttg gtgtcaatgc cttctttctg gaagagtgga ccaaggaagc
      781 ctctcacaaa gtctacttcc agttcctgag cccagatgca agccggcatg ttgccgtcct
      841 ggtcggtgtc cacttcgttt tgcacggtta cggctttgat ttttacacct tcctcaccat
      901 aggcttgtac gaacttgagc aggtagcggg aataaggctc caggctatgt ctgcgcatgg
      961 caccgcccag catagagccg ttggatttca tccaaccagg cgggctccag gctgaaccat
     1021 agaggaagat gtccgggttc accgccagag cttctcgaat tatgggtagg atgtattttc
     1081 gatcgtgctc gatggaaaag cgcttcagct cggggtccgg ctctccttcg tcgtagctgt
     1141 agacatgggt ggcgtaatcg ctggccccga tacaggtgcg gcagaaattg agattcagac
     1201 caccgtggcc gaatagttct tgaaagagcg ccgctctttt gtctttcggc atctcattga
     1261 acataaaaca ggcggcatcg gtaaaggcgc ccccgaagcc gacaatgcgc tgtttggttt
     1321 cggtcagcac cgtgatggtt ggtaccgctg cctgcatgct tccttgcgac tgggagacct
     1381 ggaaactcag tgtgctctcc tgcattcttg tttcggggct ggttctcacc actgcgagag
     1441 ttttcgctgt ggctttctct ttgccgccgt tcgttaagcg tgggcgattc tccggtgccg
     1501 cttgcacctt ttgcttgcgg ccgaccagga aaaagatcag gccggccagg gctagtgcta
     1561 ttagtaattt tggaatcata gtcttgtccg tttttgttgt tgaatagata gaccgggctc
     1621 attaggataa cccatggtcc aggtcgttct ttattgccgt ccaggtaatt gttgcttagc
     1681 tgccgccgca accgccgccg ccgccgcaag agctgccgcc accgcaagaa ctgccgccgc
     1741 cgcaactgct gtgaccactg ctgcaactgc tatgaccgct actgtggctg gtgtcggtag
     1801 tgctagtacc gtggtcggtg accgagttct tgtgggtgtt gccgtcgccg cttgagccga
     1861 ggaacggcca caaacctatc gacgagtcgt cagaactgcc ggaatcgcga cggttgttgt
     1921 cttttacatt gctcaggcgg ctgccggagt ggctcgaacc cgagcttgat ttgtaagtgt
     1981 ttctgccggt ttctctgcgt tttgaggagc ggcgaaaggc cagaaacatg atcaggaaaa
     2041 cgactgcgcc aatagtgaga gttatgataa atgccgtcat gggaattctc caattgtgtt
     2101 ggacgaaagg aactgtgcgg gcagtaagac tgcaccgcac agagttggtt tggttgaaag
     2161 gtcagagctc ttgtcggaga tgaccaggag tggcatccag gaccagacag aaggtcccgg
     2221 gcgccggtta gttggaggac gtttagtccg ttgagtcttc cgccgttaag tccagaagtt
     2281 ccagcacgat ttccgctact ggaccccggc aatctaccac tagcagatac tgctggctca
     2341 cctctcgcgc tctctcgcgg ctcaaggcat tgccttcatc caggtttttg gcgatctggc
     2401 aagtattgcg ggtgagttct tcgataggca gtacggcccg gtaaagacct gtgtagtagc
     2461 cggcatcgaa cgcggcgata acatctttcg aggtttccac cttgcgaagc agggctttta
     2521 gtgtgtcgaa tttgccgtct aggacatctt ggatctgatt ggtggtcttt tctgtcatgt
     2581 atttccaatt tgcttgtttg atttggtgtt ggagacctga acaatgcttg ccgcggaagt
     2641 tcggctaaca gtcaaacatt gcaggtctcc ggctgactta ctggcgattg tcttcaactc
     2701 cagccaagca cgtaggccag gcggtgcact atttccatga acaagcgcgt cagtccgctc
     2761 aggagtcctc gggtctcatt aggcatgagc acagccagaa ccagtatgaa gatgacaaag
     2821 tttgccatcg tttgcgtggt tctacggatc acctgcagga ctacgtagaa gacgataatc
     2881 aagagaatga gttggactgg atctatgtgc attttagttc tcagttgtgg ttgaaattca
     2941 gacttggctc tcggctgtca gaacaacggc tccgtgttcc gatgctgttt gcgaaacaaa
     3001 ggctgctact gcttcagctg ccggtgtcag attgatcgac tccggtgcgg cgatgcccag
     3061 tgacatgcac atctgtaagg ctgcgtctat agacatgtcg caatcggcaa ttctgtcttc
     3121 agggtaggcc accatcagtc cgctaatcgg gttgggcgga gtggggatca tcaccacaac
     3181 atagcgcttg ccgttggtgc ggttgattat ttccttagtg acgaaggcga tggtgacgcc
     3241 gccgccgcta gtgaacggta cccagaccac acgtttgaag ctttcctggg cgcctgactt
     3301 gcttaccatg tcgatgcctt tgcgcaagga gctgtagatg gcgcctatgc ctttgatgct
     3361 gtagatgcgg ccttccaccc agttgaagat gtaactgcct agtttccagg aaactaccac
     3421 gctgatggtg tagagcatga ccagcaggaa aaccaggctg aagatagggc tgtgggccgg
     3481 agagacggtg ttgggccaga attcttgcca gatgaaagtg atcaggcttc cgaaccagcc
     3541 gtcggctatc ttgagagcca ggttgaagcc ccagagaatg gctgcaaacg gaaagaacag
     3601 tccggcgccc cgcaaaatat ggcttttaac ttgagagaaa ttcatttttg caccttgtag
     3661 gttgatgatt gaacgaagtt gttgctgtga gaaaaccgag gcatagagcg agccccgggt
     3721 gcaggatggg taaatgacag gtttagttgc tagtgtctga agacggcctc tttgatcaag
     3781 ttgaaaagaa aatggacttc ctaagctgac tatttctcaa agggccctat caagagctct
     3841 taagagggct tattacatag agctaaggct tggctttttt tatagaagtg tctaaatgtc
     3901 cgtactggtc gtgcatagcg cctcttgcgc ggagcaggtg ttttgccaaa gagctgctgc
     3961 tgccgcggtt ttgcgaaatt ctttaggttg gcctcgggta gagcgtctcg gccgtcgcca
     4021 cacaatcggc ctgccaccac ttgggcttgg caagctggtt caggtagtta atcttggggt
     4081 ttttgggaaa tctcccccgc aagttttttg gattttctgg atttcgccgc ctggcgcagc
     4141 taggttggca attgtctgta caaggcaatc tttgctgctc gaggttgaga agaaagtcga
     4201 aatagtggtt ggcgcggaat tgttggacga agtagttcgt cggcattggg ccgccccttc
     4261 agcagagtga tttagcaata cagacttaat accgatgggg agcgggcttt gcctgctccc
     4321 tatttttttg tggggaagtt tttatgtcat acaatcctga gaattcaaaa ttgacaggtc
     4381 tgatcagaca ggctgaagat cgcctgcagt ccgttgagga gaggctcgta aaccttttga
     4441 tagaggccac ctatggcaag gtgggtcttt tgcacattaa cctggatgga ccgcaattgc
     4501 tgggccggaa gtgttttgat cgtatcttct atgggcgccg gttgccgagt ttgagagaac
     4561 tcaccaggct ggctgagcgg ctgaatatgg agctggaggt tttggtcttg tgccggcgtt
     4621 cacaggcaac cgtgcgtagt tttaaggctg ttagaggctt ttccagagcg cagttgcggg
     4681 agtttttact ggctgtgcag gcaagcgcga gccctggtca ggatactgtg atatccagga
     4741 gatgtcggtc tatgatcgat tggctggggc ttggccgcgc tcgcatttac tggcgaaatt
     4801 tggctatcat agccgctctt tgcggttatg ccattgatat taagcttgtt cctaaagacc
     4861 gtggcaatca ggctctgctt cagcgtctgg attatcctct ctaattgggc attggaaggt
     4921 gcagtagtcg ggccggggca ttcttggctc gactattgct ctgctctaac cgtgaagacc
     4981 gaggggaagg ctttctgttc atgaccgcta attcgaggga cgggcaagat ataatgctga
     5041 tttcttgcat gattattttg caaagaggaa gctgtgaaac ctactgtaaa gctcgatcaa
     5101 aaggccctta cccggcacaa gaagcgtttc ctgcgcttct ttcccaattc ctttcaagac
     5161 cagcaataca tcgattggga gcgcacctat aaatgggagg cgcacaaact atttcaagag
     5221 cacctggcgc gcgataagtt caaaaccttg atgaataaag gtcaatacga tgaaattgcc
     5281 atgagggcct tgcaggtgga ggcgcgcacc aatttcattt tctccttcga aaagatggcc
     5341 ttgcgtgacg gcgtcaaggc tcccggcggc gctagaacgt ttgccgaagg gctctacggg
     5401 ctgctttatg agcgtggacc attgtcgggt cgttttattc aatggcttgt agccctggcc
     5461 gacttgccgc gcaaaaaatc gcgagtgcta tcatggccgg tggcaacctt tttccctttc
     5521 attgcgcagc caggcaagca tattattctc aaaccgac
//
LOCUS       DAOQNZ010000045         5536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5898_length_5536_cov_3.617770, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000045 DAOQNZ010000000
VERSION     DAOQNZ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5898_length_5536_cov_3.617770"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1542
                     /locus_tag="PKW73_01430"
     CDS             376..1542
                     /locus_tag="PKW73_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71969.1"
                     /translation="MTTKETTVNVKIGELLVDAQIVSSAEMTEAVQVSKRLNVPIGRV
                     LTMSGCVREDVLEASLQVQRLLRDGNLSIEGAYETLKRVHEHRIELAEALTSEAQNML
                     MLDSAESLGELLLDSNIISEEDLVKAMQASFDNGVPLGSTLVLQGLLSPSLFPSILSV
                     QKNIARGITPREDGVKEVQEIFLHWLKAEESLKQGTIFKQFASDEITREDVASAAEAN
                     ARASARAPEEVKKAESQAREPERVRSDAEVQKPAPEQKRDQDMRLVDLLKQSGIFSQA
                     EVQRSYEALLRDPERSARFFLELGLISEEDLRNSLRAHALLQKGMLNKDEAIYAVRNE
                     RVTEYEKTISDVKDEKVKRYMDRQWRGRMSTVLGGALVGAVVAGFSMAKNKRGK"
     gene            complement(1607..2146)
                     /locus_tag="PKW73_01435"
     CDS             complement(1607..2146)
                     /locus_tag="PKW73_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71970.1"
                     /translation="MVKTRFKRIGWLTTLNVLLCCGFAALISGCSTHQTLDNFDLAAY
                     LKKQEGVAMYAAETRRTLEQEASEGNIESTAKLGVYYLIGYGVDTDQAKAHQYLQTAA
                     DKGNARAQLCMGMMTWQGLGAAKDPLKAYRYMLLASKGIGQDAQTARELAENIEKHLS
                     PKDAEPIKSEAGIELVPSR"
     gene            complement(2256..3437)
                     /locus_tag="PKW73_01440"
     CDS             complement(2256..3437)
                     /locus_tag="PKW73_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA71971.1"
                     /translation="MPNYVIKCARIFTPEASDFNRIYVRDDKIVHLSRHSAVKTDAGG
                     SPAVDVPQAYLQGFEELDFSELIAVPGFIDLQMNGTSTCNFWQDISADQLEALRLEQA
                     QHGVTSFLPTLITDSLENLKRHRDFLASHGLGDTGNQRGLARMPGLHLEGPCLSPQKP
                     GVHPVEHIQPLSLEVIKELVPAGSREVLLMTAALEGDPAGSVQNYLQNQQVALSLGHS
                     NATYEEAERAFARGCRLMTHTFNALPPLHHRQPGAVGAALLDERVSCCLIADGLHLAP
                     QICALIVKIKGLDRTILVTDRAAVGTSQGNLVGSSIILNEAVVNMIDWGICDLAGAVR
                     MASTNAAAAIGRAQSLGQLGSGFLADIAFLDQDLKVRKTMVGGTLTPVEKSPPLTCHR
                     Q"
     gene            3638..4132
                     /locus_tag="PKW73_01445"
     CDS             3638..4132
                     /locus_tag="PKW73_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71972.1"
                     /translation="MTDANPGSSSTSLSSVQNMDVNNLAAKLLELQEALVQSQEKNLM
                     LTAQLRELERMARDGEDLKSELVNQGLLLSDKSRENKQLHQELGRITTLLEQKLQEVE
                     DLRLATTELQHQLKTRESERDLLAVMLNEAENAQRRQHSEEQVQQVAKDTPGWLKAFK
                     GKNP"
     gene            4172..5131
                     /gene="meaB"
                     /locus_tag="PKW73_01450"
     CDS             4172..5131
                     /gene="meaB"
                     /locus_tag="PKW73_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNA71973.1"
                     /translation="MEAISKDVLSGNRRALARAISVVESGGEEASSLVRALFAHTGRA
                     HIIGVTGSPGVGKSTLVDALITECRKEDLKVAVLAVDPSSPFTGGAILGDRIRMQGHT
                     LDRGVFIRSMANRGHQGGVALATYDAVRMLEASGYQVIIIETVGVGQSELAIAQTADT
                     TVLVLMPGSGDDIQAIKSGIMEIGDIFVVNKGDLPGANKSASEITASLELNSFKTDWR
                     PPVIVSIAETGQGIDQLWSSVKNHKKFLEQEGLLESRRLERIQAELSEIVADIARRNL
                     KESLKSSSEVQEVLDGIIKREVDPHTAARKLIGSMFDQVSTSK"
BASE COUNT         1302 a         1420 c         1567 g         1247 t
ORIGIN      
        1 gctaggggcg gcattgagag ctagagaaag gctgaagatc aggcaagatg ctttgggcgc
       61 cccctgcgcc ggagaaagtc cgggcagatt aacaaaaagc cacctttttg agtgcttcat
      121 gagtgacctc gacattggaa tgtagtaaga attgatgcct tccttaatta gtatagagaa
      181 aattccaggg gcggaaaaag atttaacgag attgaggtcc cttgactgcc cggttttacc
      241 aaggaaaggc tgactttgac ggtcggtgct caggaaggga tgatagattc tcacttcata
      301 tctagtgttt agggttaaaa tatcgaaagt gcccaacata tatggcacgg cttggggatg
      361 ctcgggaatt ttactgtgac cacaaaagaa acgacggtga acgtcaagat tggtgagctt
      421 cttgtcgatg ctcaaatagt cagttctgct gaaatgaccg aggccgtgca ggtttccaaa
      481 cgactgaatg tgccgattgg gcgggtcttg accatgagcg gctgcgtgcg ggaggacgtt
      541 ctggaagctt ccttgcaggt gcagagactg ctgcgggacg gcaatctttc catcgagggc
      601 gcttatgaga ccctcaaacg tgtgcacgag catcgcatcg agctggccga ggccctgacg
      661 tcggaagcgc agaacatgct catgctcgac agcgcagaga gtctgggaga gcttcttctt
      721 gattccaata ttatttccga agaagacctg gtgaaagcca tgcaggccag cttcgataac
      781 ggcgtccccc tgggcagcac tctggtctta cagggactgc tttcgccaag tctcttccct
      841 tccatcttga gcgtgcaaaa gaatatcgcc agaggcatca cccccagaga agatggcgtc
      901 aaggaagtgc aggagatctt cttgcactgg ttgaaggcgg aagagtcgct caaacaaggg
      961 acgattttca agcaatttgc ctccgatgaa atcacacggg aagatgtcgc ttcggctgcc
     1021 gaagccaatg cccgggcatc cgccagggcg ccggaagaag taaaaaaagc ggagagccag
     1081 gcccgtgaac ctgagcgggt tcggagtgac gccgaggtgc agaaacctgc accggaacag
     1141 aagcgcgacc aggatatgag gctggttgat cttctcaagc agtccggtat tttcagccag
     1201 gcggaagtgc agcgtagtta tgaagcgctc ctgagagacc cggagcgttc ggcacgcttc
     1261 tttttggaac tgggtttgat cagcgaggag gatctgagaa attctctcag agcccatgcc
     1321 cttttacaga aaggtatgct gaacaaagac gaagccattt acgcagttag aaatgagcgc
     1381 gtcaccgagt atgagaagac catctccgac gttaaagacg agaaagtgaa acgctacatg
     1441 gatcgccagt ggcgcgggcg catgtctacg gtcttgggcg gggccctggt gggagccgtt
     1501 gtggcaggct tctcaatggc taagaataag cgcggcaaat agtaagagcg gcgctgatac
     1561 taaatatgat ggcacagccg gaactgcctg ggcactaaac aaaagtttag cggcttggca
     1621 ccagctctat tccggcttca ctcttgatcg gttcggcatc cttgggggat agatgcttct
     1681 cgatattttc cgctagttcc cgggcggtct gggcgtcctg cccaatgccc ttactggcca
     1741 ggagcatata gcgataggct ttgagcggat cttttgctgc tccaagaccc tgccaggtca
     1801 tcattcccat gcaaagttgg gctctggcat tacccttgtc ggcggcagtc tgcaagtact
     1861 ggtgagcctt ggcctgatcg gtgtcaaccc cgtagccaat caggtagtaa actcccagct
     1921 tggcggtgga ctctatattg ccttccgagg cttcctgctc cagagtgcgc cgggtttccg
     1981 ccgcatacat ggcaactcct tcctgcttct tgaggtaggc tgccaggtcg aaattatcaa
     2041 gagtttgatg ggtgctgcag cctgaaatca atgccgcaaa tccgcagcaa agcaagacgt
     2101 tcaaagtcgt cagccaaccg atgcgcttga acctggtctt gaccatagtc accctgagga
     2161 agggaacgag aaacgttccg gccttaccac tataaagcaa cctggcttcc ggctgcaaat
     2221 gaatagtcga tagtttagca gacttgaatc tgtcactact gccggtggca ggtcaggggc
     2281 ggtgatttct ctaccggtgt aagagttccg cctaccatgg ttttccttac tttcagatct
     2341 tgatcgagga aggcaatatc ggccaggaaa ccgctgccca gttgtcccag gctctgcgcc
     2401 cgtccgatgg cggcggcggc atttgtggag gccatgcgca ctgctccggc caggtcgcag
     2461 atgccccagt cgatcatatt gaccacagcc tcattgagaa tgatggagga gcccaccagg
     2521 ttaccctggc tggtaccgac ggcggctctg tccgttacca gaatggtgcg gtccagcccc
     2581 tttatcttga ctatcagtgc gcaaatttgt ggtgccagat gcagaccgtc ggctatcagg
     2641 cagcaactga ctctctcatc gaggagagcg gcacccacgg ctcccggctg acggtgatgc
     2701 aggggcggca gggcgttgaa ggtatgggtc attagccggc agccccgggc aaaagcccgc
     2761 tccgcttctt cgtaggtagc attactgtgt ccgagtgaga gggccacctg ctgattctgc
     2821 aaatagttct ggaccgaacc ggcggggtct ccttccaggg cggccgtcat gagcagtacc
     2881 tcacggctgc ctgccggcac cagttccttg atcacttcca ggctgagggg ctgaatatgc
     2941 tccacggggt gaactcccgg cttttgtggg gagaggcagg gaccttccag atggagaccg
     3001 ggcatgcggg ccagtcctct ctggttgccg gtgtctccca gcccatggct ggccaggaag
     3061 tctctgtggc gcttgagatt ctccagcgag tcggtaatga gcgtgggcag gaaacttgtg
     3121 acgccgtgct gcgcttgctc gaggcggagc gcttccaact ggtcggcgct tatatcttgc
     3181 cagaagttgc aggtgctggt accgttcatc tgcaggtcta tgaaacccgg tacggcaatc
     3241 aattcggaaa agtccagctc ttcaaagccc tgaagataag cctgggggac atcaaccgct
     3301 ggggagccgc cggcatcggt tttgacggct gaatgcctgg aaaggtgaac tattttgtcg
     3361 tcccggacat agatgcggtt aaaatcgctt gcctccgggg taaatattct ggcacacttg
     3421 attacatagt tcggcatttg gaaagacctt cggcattctt gttgaacaag cgtttcaaag
     3481 tgcagcttgc tcggccgaca ctccggtacg gctcaagtaa tggtaacccc agggtatctt
     3541 tccggcaaac ttctccatcg gatacaatta tccgatgaga gagtactcta atatcgagaa
     3601 ctgccagtca atttagactg agaggctaaa agggactatg accgacgcta atccaggttc
     3661 atcctccaca tccctgtctt cggtccagaa catggacgtc aataaccttg cggcaaagct
     3721 cctggagctg caggaagcgc ttgtgcagtc acaggaaaag aacctgatgt tgaccgccca
     3781 gttgcgggag cttgagagaa tggctcggga cggtgaagat ttgaagtcgg aactggtaaa
     3841 ccagggtctc ttgctttccg acaaaagtcg ggagaacaaa cagttgcatc aggaactggg
     3901 ccgaatcacc accttgcttg agcagaagct gcaagaagtg gaagatctgc gcctggccac
     3961 cactgaactg cagcatcaac ttaagacaag agagtccgaa agagacttgc tggctgttat
     4021 gctcaatgaa gcggaaaacg ctcagcgccg gcagcactcg gaagagcaag tgcagcaagt
     4081 ggccaaagac accccgggct ggctgaaagc cttcaagggt aaaaacccct aacttactaa
     4141 cgctatatag aacaccaggt tggaagagac agtggaagca atatctaaag acgttctttc
     4201 tggcaacagg agagcactgg cccgggccat ttccgtggtg gaaagcggtg gagaggaagc
     4261 ctccagcctg gtgcgggccc ttttcgccca taccggcagg gcacacatta tcggagttac
     4321 cggctccccc ggtgttggca agtcgactct ggtggatgcg ctgatcaccg aatgtcgcaa
     4381 agaggatttg aaagtcgccg ttctagccgt ggatccttcc agtcccttta ccggtggcgc
     4441 catccttggt gaccgcattc gcatgcaggg ccataccctc gaccgcggcg tcttcattcg
     4501 ttccatggcc aaccgtgggc accagggcgg cgtcgccctg gccacctatg atgccgtgcg
     4561 gatgttggaa gcctccggct atcaggtaat catcattgaa accgtgggtg taggccagtc
     4621 cgagctggcc attgcgcaga cggcagacac cactgtactt gtgctcatgc ccggctccgg
     4681 tgacgatatt caggccatca agtccggtat catggaaatc ggcgatatct tcgtggtcaa
     4741 caagggcgac ttgcccggtg ccaacaaatc agccagcgag ataaccgctt ccctcgaact
     4801 caatagtttc aaaaccgatt ggcgtccgcc cgtcattgtc tccattgccg agaccggtca
     4861 gggtatcgat cagttgtggt cttcagtcaa gaaccataag aagttccttg agcaagaagg
     4921 tttgctcgaa agtcggcgtc tggagcgcat tcaagctgag ctatccgaga ttgtggccga
     4981 tatcgccaga cgcaatttga aagagtcgct caaatccagc tcggaagtgc aagaagtgct
     5041 tgacggcatt atcaagcgag aggtcgatcc acacactgct gcccgcaaac ttatcggcag
     5101 catgtttgat caagtgagta cttccaagta aatagctatc gcttcgatat gcgatcgttc
     5161 aagcagcttg gataaatttc aataagcttg tatcgccgta gatatgccgc atacagcagt
     5221 gcaggtcgtt tccttatggg attgtcataa aattttatgg cggttgcata tatggtgcgt
     5281 atcccagccg gcttgacgtg ggcattgcca gacaatagga ttacactcgg tggagcttta
     5341 cataagttct tcttgcatat aacacagttc ttcaggcgga ctctcttcgc atgtcaaaaa
     5401 ttgccatcat cagtcaacca gatcagggcg tcttcatcga tctcggcggt tgttcgagcc
     5461 tgcaagaagc gcttgagcac ttgagttcga ccttgcagtc ttccaataaa ttctggaagg
     5521 gttcgcgcgt cattct
//
LOCUS       DAOQNZ010000046         5519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5914_length_5519_cov_3.108895, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000046 DAOQNZ010000000
VERSION     DAOQNZ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5914_length_5519_cov_3.108895"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..3617)
                     /locus_tag="PKW73_01455"
     CDS             complement(168..3617)
                     /locus_tag="PKW73_01455"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71974.1"
                     /translation="MSFNFLKKGATTNRYPTDILIGELFVKAGIITQKQLDDTIKNAA
                     TKHLHVGQMLTMAGFINHRDLQAAVDAQSMMRDKVIDMAIAARCLKIAVKTGASFDQL
                     VKEEIAIEQQASTKTNRLGEMLMDANIINKGQFGQAMGRSLATGLPLGRILVLNNSIP
                     DHLLRLALELQVRVRDGMMEREEALDALRAEAIKSAVEAPSEESSAAIDALKPIQKKK
                     IRLGELLVKANFLTDTDVISCLELGLSSDRPIGEIMVDQGYISYEMLSVALAVQQVVE
                     EDRYTAEEAAEAIKHMQNTGRIFPEIQDRINGTYVPPSEAQSSPGYEIKEPVSPRASS
                     SKLQPLYDGPELHGDYGQNDQNAYESALDGGSMGISDDAPSLATLMRSVGGDSFDEPV
                     SERLREPDNEKSLEQLLASAEQSAVDRASQSDDPQFQTGDRIAAIVHDRGAEEPVSSA
                     PAKSASTGAASFEALLLRSNVVSNSDIAAALELARQTPEMFVDILKLTGYLTEAGKNA
                     ALEAYRLEKAGELDAEQSAQVLDYCINQGRLRNLTLEGAMLELGWTRDADEVAEAASG
                     TLAEESAEPVNGQESPSDLASVLDRMSSSLDKMSALSSSEPQQAVAEAASAPSLEELM
                     ASVSGNEEAVEQPEPAAAESDGDLLKMFGLTGEAASPVDAEQLAESGAAADALIASLA
                     TAPSVEAELAEAEAASANPTAFETTAVFKQVSEAEVDEVTSKVVDDSLSLDSLLSKLE
                     EPGEFVPADSAAKAHMEASGSLANQGALPSVAALGVAADGAVPEPGLEDLMSSLSASL
                     QPSSLTTSDSPSPATVSASAAAGQELSASAADTQSGGAESGTAGAAEDMDLRSLFAGL
                     SGSGAKEQAAPQTAVETPAPEPAPAPAPAPAPAPEPAPAPAPAPAPAPAPAPAPAPEP
                     PPAPAPAPAPAPAPAPAPAPEPPPAPAPAPAPAPAPAPAPAPAPAPVRPAASSSRLPK
                     MSEADVNAAKLAQIAAMSSALTGMAEMYYEQENFPEAQKAYERILSWRQSELGPNHPD
                     VVDDLNNLAGVICVQGNFAEAEPLIRRAVEILESFEKPEPLKLAENLTSLAGLQFQQG
                     VFDKAEPLLAKALALREQELGADNPELADSLRDFAKLLKKLGKVEEAEKHYHRAKVLL
                     GKA"
     gene            4081..4713
                     /locus_tag="PKW73_01460"
     CDS             4081..4713
                     /locus_tag="PKW73_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA71975.1"
                     /translation="MELETRILIVEDHHATLDGLTLGLESQPGFKVVGSATTSDEGLK
                     LLKETCPDVVVLDLHLPGSLGPRAALEAFTAQSKEKTRLIIFSAEARVAFIQSVLAMG
                     VSAYLLKSERVAKVADTVRAVMEGKSGIVSDEVTKTYKKITRAEMEVLSMLGRGMKYQ
                     DIADHRLTSVATARKQCETLQLKLGLSSREQLIAWAVQNGFGSVDIEAVN"
     gene            4762..>5519
                     /locus_tag="PKW73_01465"
     CDS             4762..>5519
                     /locus_tag="PKW73_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71976.1"
                     /translation="MSDFKNFIKTQMGSGKDPPDPDDDLSRNLQAQFTTHARIPLEGQ
                     IDSYSGRYPGATIDDRRMQEWLFNQAAELSRALLLHAQHYYASKLKESKNSGSTGMEL
                     HFSNAARIAEPVDWISKQEQRSKIKLPSHYFRWKATGSLILSTRAGAGLIEFFIVPPK
                     EVPHITMSEFGSRFRGRFVLNQTARGFVWTNNKVRLSPEAACHLVERYLDELLEFGSP
                     RERERQSPLPVDHFTFRAAGQQRLELEKNNLLFKL"
BASE COUNT         1290 a         1556 c         1472 g         1201 t
ORIGIN      
        1 accgcctctt ctccgtccat tacgacaccg tgcatgcgct cgaacacctg agccagcaca
       61 tcggtcatgc ccaactcacc cggcagttgt gggcgctggc cgacgcagcg caaagtgcat
      121 aacgcaagct ggagcatgca tacacctcaa caccggcaag cggagcgtca agctttaccc
      181 aaaaggacct tggcccggtg gtagtgcttc tctgcctctt caacctttcc tagtttcttc
      241 aacaacttgg cgaaatcgcg taaagagtcg gccaactcag gattgtccgc gcccagttcc
      301 tgttcgcgca gagccagcgc cttggctaaa agcggctccg ctttatcaaa cactccttgc
      361 tggaactgca gcccggcaag actggtgaga ttctccgcca acttgagcgg ttccggcttt
      421 tcaaagcttt ccaggatctc aacggcacga cgaatcaaag gttccgcttc ggcaaaattg
      481 ccctgcacac aaatcacccc ggccagattg tttaaatcat ccaccacatc cggatgatta
      541 gggccaagct cactttggcg ccaggaaaga attctttcat aggccttttg agcttcgggg
      601 aagttctcct gttcgtaata catctcggcc ataccggtga gagccgaaga catggccgct
      661 atctgagcca atttggcggc attaacatcg gcctcactca tcttgggaag gcgggaactg
      721 gaggccgccg gtcgcactgg agccggtgct ggagctggtg ctggtgctgg tgccggtgct
      781 ggcgctggcg caggtgctgg tgctggtggc ggttctggcg ctggcgctgg cgctggcgca
      841 ggtgctggtg ctggtgccgg tgctggagct ggtggcggtt ctggcgctgg cgcaggtgct
      901 ggtgctggtg ccggtgctgg tgcaggtgct ggagctggtg caggttctgg agccggtgcc
      961 ggtgctggag ctggtgcagg tgcaggttct ggagccggtg tctcaactgc ggtctgggga
     1021 gccgcctgtt ccttggcgcc ggaccctgac aaacctgcaa acaaagatct caaatccatg
     1081 tcttccgcag caccggcggt accggattcg gcgcctccgg attgtgtatc agcggcagaa
     1141 gcactcagtt cttgccctgc agccgcagaa gcactgactg tggcagggga gggcgaatcg
     1201 gaggtggtca gggacgaagg ctgcaaactg gcggacagag acgacatcaa atcttccaga
     1261 ccgggctccg gtacagcacc atcggcagca acaccgagag ccgctactga aggtagagcc
     1321 ccctgattgg ccagagatcc ggaagcctcc atgtgcgctt tggcagctga atccgccggc
     1381 acaaattcac ccggctcttc caatttgctg agcagactgt ccagggacag ggaatcgtca
     1441 acaaccttac tggtcacctc atccacttcc gcttcagaga cttgtttgaa tacagccgtg
     1501 gtttcgaaag cagtgggatt ggcactggcg gcttccgctt ccgccaattc ggcttccacc
     1561 gagggggcgg tagccaggga agcaatgaga gcatcggcgg cggcaccact ctccgccaac
     1621 tgctcggcat ccacagggga agcagcttcg cctgtcagtc cgaacatctt gagcaaatca
     1681 ccatcactct cagccgccgc tggttccggc tgctcgacag cttcttcatt gccggagaca
     1741 ctggccataa gttcttcaag actgggcgca cttgccgctt ctgccactgc ctgctgaggt
     1801 tcggacgaag acagggccga cattttgtcc aggctactgc tcatgcgatc cagcactgat
     1861 gccagatcgg aaggcgactc ttgaccattc accggttcag cgctttcctc cgccaaagtg
     1921 ccggaagcag cttcagcgac ctcatcagcg tctctggtcc aacccagttc cagcatggca
     1981 ccctcaaggg taagattcct gaggcgcccc tgattaatac aataatctag aacctgagca
     2041 gattgctccg catcgagttc tcccgccttc tccagacgat aagcttccag agctgcattc
     2101 ttgcccgcct ctgtcaaata gccggtcagc ttgagaatat ccacgaacat ttccggggtc
     2161 tgcctggcca gttccagcgc cgcggcaatg tcgctattgc tgacaacatt ggaccgcaag
     2221 agcaaagcct caaacgaggc tgcaccggtt gacgcggatt tggcgggggc gctggagaca
     2281 ggctcctcag cccctctatc gtgcactatc gcagcaattc tgtctccggt ctgaaactgg
     2341 ggatcatcgg actggctggc ccggtcaacc gcagactgct ccgcggaggc cagcagctgc
     2401 tcaagggatt tttcattatc cggctcccgc agacgctcgc tgacgggctc atcgaaagaa
     2461 tcaccaccaa cggaacgcat cagtgtggcc agcgacggcg cgtcatcgga aatacccatg
     2521 gaaccgccgt ccagggcaga ctcataagcg ttctgatcgt tttgaccgta gtctccgtgc
     2581 aactccggac catcataaag aggttgtagc ttgcttgaag aagcccgggg cgacaccggc
     2641 tccttaattt catagccggg actgctttgc gcttcggaag gaggcacata ggtgccgtta
     2701 atacgatctt gaatttcggg gaaaatgcgg ccggtgttct gcatgtgctt gatggcttcg
     2761 gcagcttcct ccgccgtgta gcggtcttct tccaccactt gctgtacagc cagtgccaca
     2821 gaaagcatct cgtaggaaat atagccctga tccaccatga tttcgccgat gggacgatcg
     2881 ctggaaaggc ccagttcaag acagctaatc acatcggtat cggtaaggaa attcgccttt
     2941 accaatagtt ctcccaggcg gattttcttc ttctgaattg gtttcaaggc atcgatggcg
     3001 gcagaggact cctcagaagg tgcttccaca gccgatttaa tcgcctcagc gcgcaaagca
     3061 tcaagcgctt cttcgcgctc catcatgccg tcacgcacac gcacctgcaa ttccagcgcc
     3121 aggcgcagca aatgatccgg aatggaatta tttagtacca gtatgcggcc taaaggcaga
     3181 ccggtagcca ggcttctgcc catggcctgt ccaaattgtc ccttgttgat aatattggca
     3241 tccatgagca tttcgccaag acgattggtc ttggtggagg cttgctgctc gatggcgatc
     3301 tcttccttga caagctgatc gaaagaggca ccggttttaa cggcaatttt caagcagcgc
     3361 gccgctatag ccatatcaat caccttgtcg cgcatcatag actgagcatc gacggcggcc
     3421 tgtaaatctc ggtgattgat aaaaccggcc attgtcaaca tctgccccac atgcagatgc
     3481 ttggtggcgg cattcttgat ggtgtcgtcc aattgcttct gggtgataat cccagctttg
     3541 acaaacagct caccgatcaa aatatcggtg gggtaacgat tagtggttgc gccctttttg
     3601 agaaaattga aagacactgc ttccctcggt catccagata aaactctgtt atctaacttc
     3661 taaaattgag aggtatcctg catcaaatct ataccccaat cgcacttgca tgctctcaag
     3721 tcagacttag cggttgaata cttcgagcag tcttaagcac ttggtagaaa gtgtgtcggc
     3781 cgctcgaaaa acaattcggc tgggcaagtt gcctgacgga attgattata ttattacgtt
     3841 cccgccacag cggcactcta accgaattga aaaccaattt ctacggatac ttacgcgagg
     3901 ctgacaaacc actgcaaaat ggctcttagc aaggaaaata tcggacactt cccctcttaa
     3961 aatccttaac ataagggagc ccgccccgga gtggttccaa agattgcgaa aatttgcaga
     4021 tttaagggta catcttgctt tgaccgcacc ttactaccct tgccaacagc caccaccgat
     4081 atggaactag aaacccgcat cctcattgta gaagaccatc atgccaccct ggatgggctg
     4141 acactgggtc tggaatcaca gccaggcttc aaagtagtgg gcagcgccac gacctcggac
     4201 gaaggtctga agctattgaa agagacctgc cccgatgttg ttgtgttaga ccttcatctg
     4261 cccggcagcc tgggaccgag ggcggccctg gaagcgttca cagcccaatc caaagaaaaa
     4321 acaaggctga taatcttttc cgcggaagca agagtagcct tcatccaatc ggtcctggcc
     4381 atgggtgtat cggcctacct gctcaagtca gagagagtgg cgaaagtggc agatacagtc
     4441 cgggccgtca tggaaggaaa aagcggcata gtatccgacg aggttaccaa aacctataag
     4501 aagatcactc gggctgaaat ggaagtcctg agcatgctgg gtcggggcat gaagtatcaa
     4561 gatattgccg accacaggct gacttcggtg gctacggcaa gaaagcagtg cgaaactttg
     4621 caactaaaat tgggactttc cagtcgagaa cagttgatcg cctgggcggt tcaaaatggc
     4681 ttcggcagcg tcgatatcga agctgttaat tgaggaaacc gcccaaacgt ttatcatcaa
     4741 accgccagaa caattgccaa aatgagcgac ttcaagaact tcatcaaaac ccagatgggg
     4801 agcgggaaag atccacccga cccggatgat gacttgagcc gcaatctgca agctcaattc
     4861 accacccatg ccagaattcc gctggagggg cagattgaca gctactccgg tcgctatccg
     4921 ggagccacaa ttgatgacag gcgcatgcaa gagtggctgt tcaatcaagc ggccgaattg
     4981 tctcgggccc tgctcctgca tgcccagcat tattatgcat ccaaactgaa agagagcaag
     5041 aactcaggca gcaccggtat ggaattgcat ttcagcaatg ccgcccgcat cgccgagccg
     5101 gtggactgga tctccaaaca agagcaacgc tccaaaatca agctcccatc tcattatttt
     5161 cgctggaagg ccaccggatc actcatatta agcaccagag caggagcggg cctcattgaa
     5221 ttttttatcg tgccacccaa agaagtaccg cacattacca tgtcggagtt cggctcgcgc
     5281 ttccgcgggc gcttcgttct aaatcagacg gcgcgcggct ttgtatggac gaataacaaa
     5341 gtgaggcttt caccggaagc agcctgccat ctggtggagc gctatctcga tgaattactg
     5401 gagtttggta gcccgcgaga acgagagcgg caaagtcccc tcccggttga ccacttcacc
     5461 tttagagcgg ccgggcagca aagactggaa ctggaaaaga acaacctgct cttcaaact
//
LOCUS       DAOQNZ010000047         5478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5985_length_5478_cov_4.323437, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000047 DAOQNZ010000000
VERSION     DAOQNZ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5985_length_5478_cov_4.323437"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PKW73_01470"
     CDS             complement(<1..422)
                     /locus_tag="PKW73_01470"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNA71977.1"
                     /translation="MPRPIAWVSTISKDGVLNLAPFSFFNVVSSDPAILVFSPGFKLV
                     MQDGKKVTVKKDTLKNIQETGEFVVNVVTYALAENMNLTSGEFESHISEFEKAGLTAA
                     PSHMVRPPRVAESPISMECSLFQLIEFVQSPGAGNLVMG"
     gene            complement(475..1839)
                     /locus_tag="PKW73_01475"
     CDS             complement(475..1839)
                     /locus_tag="PKW73_01475"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA71978.1"
                     /translation="MKTAQVSGTKDMPFNEAEQKPLPASADTNEAVDSNLSMVSVYGI
                     TNRRLSQESISPPTNSEKATTKPAVYFYRQDVILNKNQDQATTEATAKSNVTILSEAD
                     FHRGLRESMKAHNGRLGVYIPGVRNLPGEAALSAAQIQSLSGEAFVVEDWVTTAKEPG
                     LNVGIFQEIDKDYQSSYDSQPMINASLKDLANLYGDNNLDLVAHSRGSVNLARALATL
                     QRETNHSVRSATFSHSDIDVNDFSHSLPQFYGAARHMTIISSGQDRALKISTMRRKLE
                     AGILSGSTDGAMILPDGAIMKTGNSLGVVGLADDRLVKVAQTLSPYKDYVSWKQETGV
                     GILRHTPEYQNIAEAITQAPSIDVKPRVMSFNEQTTDLAGVRFDLVTRFGHSVPLLWR
                     DLVSSSPELRKGNPPNLMLGNPLRSAQLENTKPPANLCTRLVYNHSNNVRAGWLRYTL
                     SRAN"
     gene            1960..3228
                     /locus_tag="PKW73_01480"
     CDS             1960..3228
                     /locus_tag="PKW73_01480"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA71979.1"
                     /translation="MKSLSVMLGVYLSLAAFCGASAQRATPARPLAFESAKGKAALGK
                     RVDQVVAQAIRRKTIVGTVVLVQKDGRLVYSRAAGFLDRENRIPMRKDAIFRLASMTK
                     PVVSAAALALVDQGKLKLDEAVTKYLNFRPRLADGREPVITIRHLMTHTAGLDYGFMQ
                     DEDGPYHLARVSDGLDMPGLSLEENLSRIASVPLLYEPGSDWQYSVATDVLGAVLEKC
                     AAKPLPELVESLVTGPLSMKDSSFSLPAGKLRRLAVPYADGKPPVRMPDAEPTLVPFP
                     GKGYLHFVPARLFDRASFASGGGGMIGTAADYLRFLNSIAGGGAPVLRKESGEAMLNS
                     QTGALKLKGQPPGWGFSYGYAVLVDRDAAHTAHENGTFKWGGAYGHAYFVDRKQRLII
                     LIMTNTAVEGTLGAFPRQVRDAVYEAAVEP"
     gene            3373..5463
                     /locus_tag="PKW73_01485"
     CDS             3373..5463
                     /locus_tag="PKW73_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA71980.1"
                     /translation="MSKGHKGFLLSGLALSFIGVSLGSFCLGPGCRPVSAQSLTQGSD
                     VNRPIRDKWALVVGISEFANPQLNLKYSAKDARDFADYLVKEAGFAPDHVKVLLNKDA
                     TERRILSELGDRWLPRVANPDDLVVLFVSTHGSGAELDVGGQNYLVAYDTDVQDLYTT
                     GVPMRRLAEDIKNRVHCDRVVIFLDACHSGGAKSEAKGLVRTGVDASELAVGSGQLVI
                     ASSAEDQVSWESKNGTNGVFTSALLESLRKKGQSTTIGEMFKDLKDKVQDQVLRERGR
                     LQTPVMESKWQGNDLCIAAAPVSRRPGLTLEPPVLKTSMEPSTVPVSAPVTVPAAAAA
                     PVAPVAATIVTPTAAIKPGILIVPGVSVGRTKIGMSKQEVEALLGRPGQENVDMFTYR
                     TSDRRYFLSLRFSGDKVSEIAFSSPAFSTASGLSLESIKHRTEELTESGRSGPYQIKV
                     GKGGGLSFIGKEGAPAQFAIVHQGPPTDPQRWLADLNTRSLSTTATAVSDTVKKSGSG
                     SASTDNPNLLRPGRSLAKVNLGMSREQVVLLLGRPTDAKGSVISYWTGDRLHFIAFHF
                     GGQQGNSLTDILFTSPSFVTAGGINVANFESANATQFGPAHMASGRPAQIYTLKEGGL
                     SFFKPASFPRAIGWLHATDSAADDLAFMDEVRADLMPGKPRLQKENAAIGPRGRRRLN
                     SLMQMSDLVNRRPY"
BASE COUNT         1152 a         1384 c         1573 g         1369 t
ORIGIN      
        1 cccatgacca gattgccggc gccgggagat tgcacaaatt ctatgagctg gaaaaggctg
       61 cattccatgc tgatagggct ttccgctaca cgcggcggtc gcaccatatg tgatggcgcg
      121 gccgtcagcc cggctttctc aaattcgctt atatgacttt caaactcgcc gctggtcaga
      181 ttcatatttt ccgccagggc ataggtgacc acattaacta caaactcgcc ggtctcttga
      241 atgttcttga gagtatcttt tttgaccgtg acctttttgc cgtcttgcat aaccaatttg
      301 aaaccgggag agaagactag aatggccgga tcgcttgaga ccacgttgaa aaacgaaaat
      361 ggcgccaagt tcaggacgcc atctttactg atggtcgaaa cccaggcgat tggacggggc
      421 atgacgcttc ccgttagcag ccggtagcga gtttcgatgg gagttttttg aatgtcaatt
      481 agcacggcta agagtatacc ggagccagcc ggctcgaaca ttatttgagt gattgtagac
      541 tagtctggta cagaggttgg ctggcggttt tgtgttttcg agctgggcgg atcgcagtgg
      601 gtttccgagc attaaattgg gaggatttcc tttacgcaat tcagggctac tggaaacaag
      661 atcccgccat agcaagggca ctgaatgtcc gaatcttgtg actaaatcaa agcgaacacc
      721 agctaagtcc gtggtttgtt cattgaagga cattacacgt ggtttaacat cgatggaagg
      781 cgcctgagtt atcgcttctg ctatattctg atattccggg gtgtgccgaa gaatgcctac
      841 tccagtctct tgtttccatg aaacgtagtc tttatacggt gaaagagttt gggctacttt
      901 cactagcctg tcgtcggcta gtcccacaac cccgagagaa ttgccggttt tcattatcgc
      961 accgtcggga agaatcatag caccatccgt ggagccgctt agaatgcctg cttccagttt
     1021 ccttcgcatg gttgaaattt taagtgcccg atcctgaccg gaggaaataa tcgtcatatg
     1081 ccttgctgct ccatagaact gcggcaagga atggctgaag tcgtttacgt cgatatcaga
     1141 gtgactaaaa gtagcacttc tgactgagtg gttagtttcc cgttgaagtg tggccagggc
     1201 tctggcaaga ttgactgagc cccgactgtg cgctacgaga tcgaggttat tgtctccgta
     1261 gagattggcc aggtctttga ggctggcgtt gatcatgggc tgactgtcat aggaagactg
     1321 atagtctttg tcgatttctt gaaaaatacc gacgttcagc cctggttcct tggctgttgt
     1381 gacccagtct tccaccacaa aagcttcgcc gcttagggac tggatctggg cggcgcttaa
     1441 ggctgcttcg cctggtagat tgcgcacgcc tggtatgtaa acaccgagac ggccgttatg
     1501 ggctttcata gattcccgga gacctctgtg aaaatccgct tcgcttaata tggtcacatt
     1561 gctcttggcg gtagcttcgg tggtggcctg gtcctggttc ttatttaaga ttacgtcctg
     1621 tctgtaaaag taaaccgccg gtttggtggt tgctttctcg gagttggtcg gaggtgaaat
     1681 agattcttga ctgagccgac gattggtaat gccgtagaca ctcaccattg acagattcga
     1741 atctaccgcc tcgttggtat cggcggaagc tggaaggggt ttctgctctg cttcattgaa
     1801 gggcatatct tttgttccgc taacttgagc agtcttcata gtagtgggaa attcgcctgc
     1861 tgtaattagg gaaactacgt agtcgccgga ggaatccgct ttcttctccg gcggggggta
     1921 aaatggtcca attcttactc ggaaaacggg gattcaccgg tgaaaagtct gtctgtaatg
     1981 cttggggttt atctgtcgct ggcagcattt tgcggcgcct ctgcccaaag ggccaccccg
     2041 gcccgtcctc tggcttttga atcggcaaaa ggcaaagccg cactgggcaa aagagtcgac
     2101 caggtggtcg ctcaggctat caggcgcaag accatagtgg gcacagtcgt gctggtgcaa
     2161 aaggacggtc ggttggttta cagccgtgcc gccggtttct tagataggga aaaccgcatt
     2221 cccatgcgta aggatgccat ctttcgcctg gcttccatga ccaagccggt agtttctgcc
     2281 gctgccctgg ccctggtaga tcagggcaaa ttgaagcttg acgaagccgt cactaagtac
     2341 ctcaattttc gtccgcgcct ggcagacggg agagagccgg ttatcaccat caggcatctg
     2401 atgacccata ctgccggact ggattacggt ttcatgcagg atgaggacgg accctatcat
     2461 cttgcccgcg tcagcgatgg gctggacatg cctggtttga gtcttgagga gaatctttcc
     2521 cgcattgcat ccgtgccgct tttgtacgag cccggtagcg actggcaata cagtgtcgct
     2581 accgatgtgc tgggcgcggt tctggaaaaa tgtgccgcta aacctttgcc tgaactggtt
     2641 gagagcctgg tgaccgggcc tctgtccatg aaagatagtt ccttctcttt gcctgccggc
     2701 aagcttcggc ggctggccgt cccctacgcc gacggcaagc cgcctgtacg aatgcctgat
     2761 gctgagccga cattggtgcc tttcccgggc aagggctact tgcacttcgt gcccgccaga
     2821 ctctttgata gagccagttt tgcctcagga ggcggcggta tgattggcac tgccgccgac
     2881 tatctgcgct ttctgaatag tattgccggc ggtggcgccc ctgtattgcg aaaggagagt
     2941 ggagaggcga tgctaaatag ccagacgggc gccttgaaat tgaaggggca gccgcccggt
     3001 tggggcttca gctacggcta tgcggtattg gtcgacaggg atgctgccca tacggcgcat
     3061 gagaacggta ccttcaagtg gggcggcgcc tacgggcacg cctattttgt cgatagaaaa
     3121 caaaggctaa taatcctaat tatgaccaat accgctgtag aaggaaccct gggcgccttt
     3181 ccccggcagg tgagggatgc tgtctatgaa gcggcagtgg aaccctaaaa agtcttgaat
     3241 tgggcgcagt tggcggaatt tccttgcatt tgacaagctt tatcagggtc tttccgatta
     3301 tggctgtcgg cagtgcgaaa atataggggt ggttttcatg ggtatgacct ggtgagcttg
     3361 ggcgagttta aaatgagtaa agggcacaag gggtttctac tttcgggttt ggcactttct
     3421 tttattggtg tgtcgctcgg ctccttctgc ctgggccctg gctgccggcc ggtgtcggca
     3481 cagtctctaa cccaggggtc cgatgtcaat cgccccattc gcgataaatg ggcgctggtg
     3541 gtaggcatca gcgagtttgc caatccccag ttgaatctca agtattcagc caaagatgcc
     3601 cgtgacttcg ccgactatct tgtgaaggaa gccggctttg ctcccgacca tgtgaaagta
     3661 ttgctcaata aggatgccac ggaaaggcgc atcctctcag agcttggcga tcgctggttg
     3721 ccccgggtgg ctaacccgga tgacctggtg gtgctgtttg tttccaccca tggcagcggc
     3781 gccgaactgg atgtgggcgg acagaattac ctggtggctt atgataccga cgtgcaggac
     3841 ctgtatacga ccggtgtgcc catgcgccgc ctggctgagg acatcaaaaa ccgggtgcac
     3901 tgcgatcggg tagtcatttt cctggatgcc tgccatagcg gcggtgccaa gtcggaggcg
     3961 aaggggctgg ttcgcaccgg tgtggatgcc tcggaattgg cggtcggttc cggacagctt
     4021 gtgattgcat ccagcgcaga agatcaggtt tcctgggaaa gtaagaatgg taccaacggt
     4081 gtttttactt ccgctctcct agagtcgttg cggaaaaaag gtcagtcgac gactatcggc
     4141 gagatgttca aagacttgaa agacaaggtg caggatcagg tcttgcggga gaggggccgt
     4201 ctgcagaccc ctgtgatgga aagtaagtgg cagggaaatg atctgtgtat cgccgctgct
     4261 cctgtttctc gccgacccgg tctcaccctt gagccaccgg tgctcaaaac cagtatggag
     4321 ccgtccacgg taccggtatc tgcaccggta acagtaccgg cagcggcggc agcgcccgtc
     4381 gcgccggtgg cggctacaat cgttacgccg actgctgcca ttaagcccgg cattttgatt
     4441 gtgcctggag tttcagtcgg tcgtaccaaa atcggtatga gtaaacagga agtggaagca
     4501 ctgcttggca gacccgggca agaaaacgtg gatatgttta cctatcggac atcggaccgg
     4561 cgctacttcc tgtccctgcg tttcagtggt gataaggtat ccgagatcgc cttttcttct
     4621 cccgcctttt ctacggcttc cgggctcagc ctggagagca tcaaacatcg gacggaggag
     4681 ttgactgagt cagggcgttc cggaccttat caaatcaaag tcggcaaagg tggcggtctt
     4741 tcttttatcg gaaaggaagg ggcgcccgct cagtttgcca tagtgcacca gggaccgccc
     4801 accgatccgc agcggtggtt ggctgatttg aatactcgca gcctgtcgac taccgccact
     4861 gcggtatcgg atacggtgaa gaagtcgggc tctggttctg ccagtacaga taatcccaat
     4921 ctacttcgac cgggacgttc gctggccaag gtcaacctgg gtatgagtcg agagcaagtg
     4981 gtgctgctgc tgggtcgccc cactgatgcc aaaggctcgg ttatatctta ttggaccggc
     5041 gaccgcctgc atttcattgc cttccatttc ggcggacagc agggaaattc gctcacggat
     5101 atactcttca cttcgccctc tttcgtcact gccggcggta tcaatgtggc aaacttcgaa
     5161 agtgccaatg ccacgcagtt cggaccggcc catatggcta gcggtagacc ggcgcaaatc
     5221 tataccctga aagaaggagg tttgtccttc ttcaagccgg cctccttccc ccgcgccatt
     5281 ggttggttgc atgccactga ctcggcggct gatgatctgg ccttcatgga cgaagtacgc
     5341 gctgatctca tgcccggcaa gccgcgcctg caaaaagaaa atgccgccat tggcccgagg
     5401 ggcaggcgtc gtctcaatag cctcatgcaa atgtccgatc tggtaaatcg tcgcccttat
     5461 tgatgtttgt tggttttt
//
LOCUS       DAOQNZ010000048         5472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_5999_length_5472_cov_3.804320, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000048 DAOQNZ010000000
VERSION     DAOQNZ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5999_length_5472_cov_3.804320"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PKW73_01490"
     CDS             <1..1181
                     /locus_tag="PKW73_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71981.1"
                     /translation="DKPSLKLSDLPSSLPDGHQAVRFDRGDKVEHRQVEASAQTDRLF
                     SQEVQEIRQKGGAVTPEQLKQTAFRALDRLFGQASALEGSNEMSPEHSRFRADFSELG
                     KSSGKSGNARDIFRDAEARALLPMIGDNRAGLFDDSQTASPLDNVPLKNINGSFFSQA
                     DLEKIAAGGSVEISLTARDLPGGRRQGRYIRLPGSGSDVDKVVALIGQNEGKANSINW
                     NDNGAGISVGIQQSNQRGGNLPELLQRMHSANPQKFNQIFGANASRMLNENFVRNAHF
                     SPRNELGRAMVRAVNEPEFQRAQVTLVREHVQRAAEIARGLGIKSTMGVALVADLTNQ
                     FGEGGALKYLRQARGRHSEEAKITAIARASEGGHAHRRQRFNSIARSGIVSSQENFMA
                     "
     gene            complement(1178..1624)
                     /gene="aroQ"
                     /locus_tag="PKW73_01495"
     CDS             complement(1178..1624)
                     /gene="aroQ"
                     /locus_tag="PKW73_01495"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNA71982.1"
                     /translation="MKSKLLVINGPNLNRLGKRDNSLYGSLSLKDIEAGLHESFPRTE
                     LTFFQSNHEGALIDRIQEAAEDGTRGIVINPGAYGHTSIALRDAFLDVGLPFIEVHIS
                     NVYKREEFRRKSLLSDIASGVIVGLGPAGYETAVTALNRLLRQQDN"
     gene            complement(1621..2250)
                     /locus_tag="PKW73_01500"
     CDS             complement(1621..2250)
                     /locus_tag="PKW73_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71983.1"
                     /translation="MTVTQDELMYLQSQLEGLESIFMELMPFGIELKRQHVQDYYDKR
                     FDAATKPVSSVAENELRRQFNTKANQVRNLVDSAESLGDAGNRLNLIRAAASLPEERS
                     KGLLNSVLNFSKAVVMENRVETELFNEILQSKELRPVEARVLLGAAMFVIDRELPTQE
                     GINMPVIDILGELVQMVRREQLLTRNDPFLVEAQCALEALEMEEEELQS"
     gene            complement(2331..2852)
                     /locus_tag="PKW73_01505"
     CDS             complement(2331..2852)
                     /locus_tag="PKW73_01505"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNA71984.1"
                     /translation="MLQENLILLGPSGAGKTSCSKLLAERLGYLLLDTDALIEEATAM
                     SPAQIFATMGEAAFRTMENELLRQIAMEKTGSPFVMATGGGLPASENNLEILQKLGIT
                     FYLKADSATLAKRVSGDVERPMLKNGAGELTERIDTLLAKRAAFYGQSRHTVDTRGMT
                     VEETVETILNYLQ"
     gene            complement(2872..4212)
                     /locus_tag="PKW73_01510"
     CDS             complement(2872..4212)
                     /locus_tag="PKW73_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71985.1"
                     /translation="MLLTLFVAGTLVVGIPGVYFVFKDTPQAHSLAALVNQNTSKFLS
                     GLPLIHQDKAASAGLTPRMTAAAAPPANPSCEVKIVDQAVASINQALEKDPANPSLHN
                     RLGIIYAEMGVMPEAEGQFKQAILLSRSKLAGVYAAIEKKKNEGLINEASTLMIEVSA
                     LQLELSSAHSNLARVFEKLGDNQRVVKQLEELNRDVTIGGGLAKTADSAGKLAPKLRP
                     EIVAGLARAEALMQVGRMQDAGREVRTLLAVAPDLAEAHELLGRTGMATSNFFLAQRE
                     LEIAARQSPQKASIFANLGLVYQYRGKPKDAIQAFSRALQLDGKDSASAFNLGNIYAS
                     AGKNQEAKKCFQQAIAINPNMAVAHNNLASMYSQSGDYEGAIRQFEQAIFLAPQMASA
                     HYGMGLAMYQMKDFAGASAAFKQAIMLNPNLIDARNKLEICLKKGGSGPSMATM"
     gene            complement(4452..>5472)
                     /locus_tag="PKW73_01515"
     CDS             complement(4452..>5472)
                     /locus_tag="PKW73_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNA71986.1"
                     /translation="TGVWFGAEQLKSIFNLVMRSGVPIEMALTILGLHLPEVIVMTIP
                     IGVLLGTLLVFNRLSGDSEIIALRTCGVSFYRIMAAPLMFGLLTSFVSLGLNEAVVPW
                     ANRTAKRLEFVALYKTELPAGQANFTYFERAKDLKLNRMFYVGYYSGKYLSNIIVLDF
                     TRDKLVQIIAATSGLWNNGEWVLEKGRTYVLAGDSDITRVLQFDKLVIPGLGTVQKVL
                     DTGKISPKEMNMLELGHYLELLKASNGQTNDLLVRYYQKFSQPLACLIVALAGAPLGL
                     LARRSRSNLGLIYSAVVVFLYYVLQSSSGALGDAGRIPPIVAAWLPNFVIGILGMIIL
                     YVKGK"
BASE COUNT         1224 a         1427 c         1524 g         1297 t
ORIGIN      
        1 tggacaaacc cagcctaaaa ctctcagatc tgccttcctc cttgccggat ggccaccagg
       61 ccgtgcgctt tgaccgcggc gataaggtgg agcatcgcca ggtggaagct tccgcgcaga
      121 cggaccgcct cttcagccag gaagttcagg aaatacgcca gaaaggcggg gctgtgacac
      181 cggagcagct caagcaaaca gcctttcggg cccttgaccg gcttttcgga caggcttccg
      241 cccttgaggg ttccaacgag atgagccccg agcacagccg ctttcgcgcg gatttttccg
      301 agcttggtaa gtcttccggc aagagcggca atgcccgcga tatctttaga gatgccgaag
      361 ccagggctct tctgcccatg attggcgaca atagagccgg gcttttcgat gacagccaga
      421 ccgccagtcc cctggacaat gtgcctctga agaacatcaa cggttccttc ttctcccagg
      481 ccgatctgga aaagattgcc gccggcggct cagtggaaat aagtttgact gctcgcgatt
      541 tgcctggcgg tcgccgtcag ggtcgctata tccgcctgcc gggcagtggc agtgatgttg
      601 ataaagtggt ggctctgatt ggtcagaatg aaggaaaggc caattccatc aactggaacg
      661 ataatggcgc aggcatttcc gtcggcattc agcagagcaa ccagagaggc ggcaacctgc
      721 ccgagcttct gcaacgtatg cactcggcca atccccagaa attcaatcaa attttcgggg
      781 ccaatgcttc ccgcatgctg aacgaaaact ttgtgcgcaa tgctcatttc tcacccagaa
      841 atgagctggg aagagccatg gtgagagccg tcaatgagcc ggaatttcaa agggcgcagg
      901 taaccctggt gagagaacat gtacaacggg ccgcggaaat cgcccgcggg ctcggcataa
      961 aatctacaat gggtgtggct ctggtggccg acctcaccaa tcagttcggt gaaggcggtg
     1021 cgctcaagta tttgcggcag gcccggggca ggcattctga agaagctaaa attaccgcca
     1081 tcgccagagc ctcggagggc ggacatgcgc acagacggca gcgtttcaac tctatagcac
     1141 gctccggtat agtttccagt caggaaaact tcatggctta gttgtcttgc tggcgcaata
     1201 gccgattcag ggccgtaacc gcagtttcgt agccggccgg accgagaccg acgatgacac
     1261 cactggcgat atcggaaagc aggcttttgc ggcggaattc ctcgcgttta taaacgttgg
     1321 aaatatgcac ttcaatgaag ggcagaccga catcgaggaa ggcatcgcgc agggctatgg
     1381 aagtgtgacc gtaggcgccg gggttaatca ctatgccgcg ggtaccgtct tccgccgctt
     1441 cttgaatgcg gtcgatgagg gcaccttcgt gattgctttg gaagaaagtc agttcggtgc
     1501 ggggaaagct ctcgtgcaat ccggcttcaa tgtctttgag gctcaaactg ccgtagagag
     1561 aattgtctcg tttgcccagg cggttcaagt tgggaccgtt gatgacgagc agcttgcttt
     1621 tcacgactgc agctcttctt cttccatttc cagtgcttcc agggcgcact gagcttccac
     1681 caggaaggga tcgtttctgg tcagaagctg ttcccgtctc accatctgca caagctctcc
     1741 cagaatgtct atgaccggca tgttgatgcc ttcctgggtg ggtagttctc tgtcgatgac
     1801 aaacatggcg gcgcccaaaa gcactcgcgc ctccactggt ctcagttctt ttgattgcag
     1861 aatttcattg aaaagctcgg tttcgacccg gttttccatg actacggctt ttgaaaaatt
     1921 gagtacggaa tttaggagcc ccttgcttct ttcttccggc aaggaggctg ccgcccggat
     1981 caagttcagt ctgttgccgg catcaccaag gctctcggcg ctatccacca ggtttctcac
     2041 ctggttggct ttggtgttga actgacgacg cagttcgttt tcagccacgg aggagacagg
     2101 cttggtggct gcgtcgaagc gcttgtcgta gtagtcttgc acgtgttgac gcttgagctc
     2161 aattccgaag ggcataagct ccataaaaat ggattccagt ccttccagtt gggactgcag
     2221 atacatgagc tcatcttggg tgacggtcaa ggataactcc ttacaagtcc aggtcaggga
     2281 acaaacgact aatcccaaat aatatcactg acggtgagcc tccatggaaa ttattgcaag
     2341 tagttcaaga ttgtctccac cgtctcttct actgtcatcc ctctggtatc aaccgtatgg
     2401 cggctctgac cgtaaaaggc ggctctcttg gcaagaaggg tgtcaattct ttctgttaac
     2461 tcaccggcac cgtttttcag catagggcgt tccacatcgc cgcttactct cttggcaaga
     2521 gtggcggagt ctgccttcaa atagaaagta atgccaagct tttgcaagat ttcgagattg
     2581 ttttcgctgg ccggcaagcc gccgccggtg gccatgacaa aggggctgcc ggttttttcc
     2641 atggcaatct ggcgcagcag ttcattttcc atggtccgga atgccgcctc gcccatggtg
     2701 gcaaaaattt gagcgggact catggcggtt gcttcttcaa tcaaagcatc cgtatctaaa
     2761 agcaagtagc caagccgttc tgccagcagc tttgaacaac tggtcttgcc cgctccggaa
     2821 ggaccgagca aaatgagatt ttcctggagc actttgctga tggcctggcc cttacatagt
     2881 agccatactg gggccggagc cgcccttctt caggcaaatt tccagcttgt ttctggcgtc
     2941 gatgagattt ggattgagca ttatagcttg tttgaaagct gccgaggcgc ctgcgaagtc
     3001 tttcatctgg tacatggcca gacccatgcc gtagtgggct gatgccatct gtggtgccag
     3061 gaaaatagcc tgttcaaact ggcggattgc gccttcatag tcgccgctct gggagtacat
     3121 ggaggctaga ttgttgtgag ccacggccat gttgggattt atggctatgg cttgctggaa
     3181 gcatttcttt gcttcctgat ttttgcctgc cgaggcataa atattgccca gattgaaggc
     3241 tgaagccgag tctttgccgt ctagctgcaa ggcacgggaa aaggcttgga tggcgtcttt
     3301 gggtttgccc cggtattgat agaccagacc gagattggca aaaatactgg ctttctgtgg
     3361 tgactggcgg gctgctattt caagctcccg ctgggccagg aagaagttac tggtggccat
     3421 accggtgcgc ccgagaagtt catgggcttc cgctagatca ggtgctacgg caagcagtgt
     3481 gcgcacttcg cgaccggcat cctgcatgcg ccctacctgc atgagcgctt ccgctctggc
     3541 aagaccggcg acaatttccg gtctcagctt gggtgccagt tttcctgcac tatcggctgt
     3601 tttggccaga ccgccgccta ttgttacatc ccggttcaat tcttctaact gtttgaccac
     3661 ccgctggttg tctcccagtt tttcaaagac cctggccaga ttggaatgtg ccgatgaaag
     3721 ttccaactgc agagccgata cttctatcat caaggtggaa gcttcgttta tgagcccctc
     3781 attcttcttc ttctcgatgg ctgcataaac gccggcaagt tttgaccggg aaagaaggat
     3841 ggcttgcttg aactgtcctt ctgcttccgg catgacgccc atttcggcgt agatgatgcc
     3901 gaggcggttg tgcaagctgg gattggccgg atctttctcc agcgcttgat tgatcgaggc
     3961 tacggcctga tcgacgattt ttacttcaca ggagggattg gccggcggcg cggcggcagc
     4021 ggtcatcctg ggtgtgagac cggcactggc tgctttatct tgatggatga gaggcagacc
     4081 ggagaggaac ttgcttgtat tctggttgac cagggccgcc aggctgtggg cctgaggggt
     4141 gtctttgaag acgaaataga cccccggtat gccgaccacc agggtgcctg ccacaaacag
     4201 ggttaagagc acccggttcg cccgacggga accagaatga agtgatttag cgatttgact
     4261 agtactagcc aaagatgttc tcgcttgacg ccagggatgt attcaatagg tccagattaa
     4321 gcttggcaca tgcccgagtc aatacgggtt tttacgcagc aggccggagc aaatagacaa
     4381 agtaaattag acgtctctta ttaatacttc agaaagagaa cagtgacatg ccgataatga
     4441 actttcttcc gctatttgcc ttttacgtag aggattatca tgcccaggat gccgataaca
     4501 aagttgggca accaggccgc cacaatgggc ggaatgcgtc cggcatcgcc taaagcgccc
     4561 gatgacgact gcaagacgta atagaggaag accaccacgg ctgaataaat aagacccagg
     4621 ttggacctcg accttcttgc cagaagtccc agaggcgctc ctgccagggc tacgatcaga
     4681 caggccagtg gttgcgaaaa cttctggtag taacgcacca acagatcatt ggtctgtccg
     4741 tttgaggctt tgagaagctc cagataatgc cccagctcaa gcatattcat ttctttgggg
     4801 gaaattttgc ctgtgtcgag aactttctgc accgtaccca gaccgggtat gaccagtttg
     4861 tcgaattgca gcacccgggt tatgtccgag tcgccggcca gcacataggt gcgtcccttt
     4921 tccaggaccc actcgccgtt gttccagaga ccggaagtgg cggcaatgat ctgcaccagt
     4981 ttgtcccggg tgaagtcgag cacaatgata ttgctcaagt acttgccgct gtaataaccc
     5041 acatagaaca tgcggttaag cttcaggtct ttggcccttt cgaagtaagt gaagttagcc
     5101 tgaccggctg gcaattcggt tttatagagg gccacgaact caaggcgttt ggccgtgcgg
     5161 ttggcccagg gcaccaccgc ttcgttcaag cccaggctga caaaactggt aagtaagccg
     5221 aacataaggg gcgcggccat gatgcggtaa aaactgacgc cgcaagttct cagggcgatg
     5281 atttcggagt cgccggagag gcggttgaag accagcaagg tacccagcaa tacaccgatg
     5341 gggatagtca tgacgataac ttccggcaag tgcaagccga gaatggtaag agccatttcg
     5401 atgggcacac cggaccgcat aaccaggttg aagatggatt ttagctgctc ggcgccgaac
     5461 cacacaccgg tg
//
LOCUS       DAOQNZ010000049         5438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6058_length_5438_cov_4.029537, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000049 DAOQNZ010000000
VERSION     DAOQNZ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6058_length_5438_cov_4.029537"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            548..1654
                     /locus_tag="PKW73_01520"
     CDS             548..1654
                     /locus_tag="PKW73_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71987.1"
                     /translation="MLRNSVDLEELKNRRSFPKNNYFCYKWRIKDHFNRSIDRQIFHL
                     RALGASNDVSLMNDRIMQLYKSSFGSSRKDLIVDLARDAMSFLDRRRSDQKRQQAKEL
                     RLSTATIELVQYASNVLLGFSTELNSLMGLSELFITASEPEAKKLGACGEGITHVVQA
                     GFSTNSFRLVIEGRLECINFYLVPADSLLTLNEIAMNYEPLEKWRSLIGDTGAVKWYS
                     GGRAIAEDMVEVICSELLRVLLAATQERLMPELESVAADESFFELSAPAPWELDLESR
                     KQSDRELPNGEMPVDSRGFSLELQPEAELFNSVSNDYYVEALETPEYELGMKSGCYPC
                     LSSAFTTTLTNVDAVPPEVGKTSKKKRRNSKGRK"
     gene            complement(1783..2550)
                     /locus_tag="PKW73_01525"
     CDS             complement(1783..2550)
                     /locus_tag="PKW73_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA71988.1"
                     /translation="MDKKTVMIMGATGCTGSALAERLCRSGYKVGLMGRSQEKLAELK
                     ERLMACGAAEEDVATALCDATVYAQVEAALVRLELQLGDTYGLVNCVGSLHLRPAHTT
                     DIDDWQEVLNTNLNSCFFLLKAGIKGLRKTRGSVVFIASAAAQTGMSDHEAIAAAKAG
                     VIGLSRSAAATYARSGIRVNCLAPGLVETNLTAAITSAQALKEASTAMHPLGRLGQPE
                     DLAGAIAFLLSDQASWITGQILGVDGGLANLRTKARA"
     gene            complement(2702..2893)
                     /locus_tag="PKW73_01530"
     CDS             complement(2702..2893)
                     /locus_tag="PKW73_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71989.1"
                     /translation="MVIYLYKEQTVRSNACLLSFGVSAEECLFMPVRSSASGTTCATT
                     SSIMPKEDKPIESTYLGTR"
     gene            3075..3602
                     /locus_tag="PKW73_01535"
     CDS             3075..3602
                     /locus_tag="PKW73_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA71990.1"
                     /translation="MTKTDITESAAIQQRLAEMQAQAVSQSEIALHWAGHNRQDLAIV
                     FINMAKLMLLEGEGLGSESEFWVLTNRAAVAEFARQPKLALDLAVRAHDLGEKLFLGD
                     ALKLAVARGNLGKALAGADRPEEARKRLSEGLFGVSTASHSPPAHTPEPLKKYYREAF
                     MDYARALQKLPARPR"
     gene            3706..4722
                     /locus_tag="PKW73_01540"
     CDS             3706..4722
                     /locus_tag="PKW73_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNA71991.1"
                     /translation="MSFASMSDSLSGLLPFYVFVATAAWGLWGIFDKLALERSQPKDV
                     FLATTLFALPQIPLLLFMLYWSQPQWQLTPSLLIYAGLSSLVSSLGMMLYLDSMKRTD
                     TTVILSLTAAYPLVTQIVCFFFLEETFSAGRALGAVFIASGVVVIGLTDNSSPQPITN
                     LPQPGKPDPSSNHEPDPSSEQKPERLPVLRSALRLFLRPGLGRLFLSAMVVNVCWGLK
                     GVFEKLSVQHGTPLESYFSECLTNLALLLPVLLWFFWSGFKPRLKSKHLWRYTGLSEL
                     ALAVGGISYLTALSLAPASYVVTITSTYPLLMYFIALFVLNEKFNRIRLFGIVCIVVG
                     GLIV"
     gene            complement(4904..>5438)
                     /locus_tag="PKW73_01545"
     CDS             complement(4904..>5438)
                     /locus_tag="PKW73_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71992.1"
                     /translation="QSQGIADAHFTQAPSKNMVVSMVENLREFGLPAAGIVDVDYLFD
                     DAAEHKRTYQKLGMATGAVDRLENLRKELRNQVNQEDKKKLKKCPRDVLNNEMYEVFN
                     SYISELANWGIFVVPVGQLESWLSRCNLQDTSKRKWLDIALDSISPTSTQQLIYPEDG
                     DVGTFINSIGKWAAKQH"
BASE COUNT         1193 a         1468 c         1396 g         1381 t
ORIGIN      
        1 aaagtgcgga ggtatttgcc ccttggcttc cgtttatagc tcttaattgc tattcatcta
       61 tttcgtttgc ggtatttcgc tcgcggtatt ccgcttgaga aagatgaaaa gcctaaaacg
      121 ccctggtaat aaggggagat gctgaccttt agcggttcat ggcaaatgcc gagccgttaa
      181 aagcctgtgg gcgtttttag cgctataacc tgtgcttgaa aggcacaagt gtttgttttg
      241 cacaaagcgt gctttagtaa gtgaataaat ggagcaaatg ccccaagtga ggctaagtgc
      301 ctcgtggatg aatttactgc accgggttgt tgctgtcaag tagatttaat gcaaaggccc
      361 ccggctctta cacgttttcg ttagtacccc ttcgccaatt tacggctacc cagcctcgta
      421 ctaggatgga ttcatgaggg tcggtaaacc caataccaaa accagcccct gcaagcactc
      481 agccaagcct tactaaagat tactcactga catgtccgct gccatagctg tggctcggga
      541 ggattttatg ctgcgaaatt cagtcgacct ggaagaattg aaaaaccgcc ggagcttccc
      601 caagaataac tatttttgct acaagtggcg cattaaagac catttcaacc gctccatcga
      661 caggcagata tttcatctgc gggccctggg cgcctctaac gatgtttcgc ttatgaatga
      721 tcgcatcatg caactctaca agtcctcttt cggttccagc cgcaaagact tgatagttga
      781 tctggctcgc gacgccatga gttttctcga tcgacgccgc tccgatcaga aacgccagca
      841 agcaaaggaa ctgcggctgt ccaccgccac cattgagctg gtgcaatatg cctccaatgt
      901 cttgctcggc ttttccactg agttaaacag cctcatgggc ttgagcgagc ttttcattac
      961 cgcctcggag cccgaagcta aaaagctggg cgcttgtggc gaaggcatca cccatgttgt
     1021 gcaagccggt ttcagcacca acagctttcg cctggtcatc gaagggcgcc tggagtgcat
     1081 caatttttat ctggtgccgg ccgacagctt gctcaccttg aatgagattg ccatgaatta
     1141 tgagcccctg gaaaagtggc gcagtctgat tggcgacacc ggcgccgtaa aatggtattc
     1201 cggtggtcgg gccattgccg aagacatggt agaggtgatt tgttcagagc ttctgagagt
     1261 gcttctggct gctactcaag aaaggcttat gcccgagctg gagtctgtgg ctgcagacga
     1321 aagcttcttc gaactttccg ctccagctcc ctgggagctc gatcttgaaa gtcgtaagca
     1381 gtccgaccgg gaattgccca acggggagat gcccgtcgac agccgaggtt tttctcttga
     1441 gctgcagccg gaagctgaac tcttcaacag cgttagcaat gactattacg ttgaggctct
     1501 tgagacacct gaatatgagt tgggcatgaa gagcggctgt tatccgtgtc tttcatcggc
     1561 atttacaacc actcttacca atgtagatgc cgtccctccc gaagtcggca agacttccaa
     1621 aaagaagcgc agaaacagca agggtcgcaa ataaaaatag agcatagttt ttcccggcaa
     1681 aaccaaccct cccataagcc ggagaagggg gcagagatta atctctgtcc ttttctcttg
     1741 ctgttttgct agaggggcaa tgctggaagt gccggctctg gttcaggctc tggctttggt
     1801 tcgtaggttg gcgaggccgc cgtctactcc cagaatctga ccggtgatcc aggaggcctg
     1861 atcactcaaa agaaaagcaa tcgctccggc cagatcttcc ggctgtccca ggcgtcccag
     1921 aggatgcatg gccgtggagg cctctttcag tgcctgcgcg gaagtaatag cagcggtcag
     1981 attggtttca accaggccgg gtgccaggca gttcactctg atgccggagc gggcgtaggt
     2041 ggcggcagcc gagcggctca gaccgatgac accggctttg gcggcggcaa tggcttcgtg
     2101 atcgctcata ccggtttgag cggcggctga ggcgatgaag accacgcttc ccctggtttt
     2161 gcgcaaaccc ttgatgcctg ccttaagcag aaagaagcaa gaattcaagt tggtattcag
     2221 aacctcctgc cagtcgtcga tatctgttgt gtgggcgggc ctcaagtgca gagagcccac
     2281 gcaattgacc aggccgtagg tgtctccgag ttgtaattcc aggcgcacca gggccgcctc
     2341 tacctgggcg tagacagtgg catcgcagag agccgtggcc acgtcttctt ccgcggcgcc
     2401 gcaggccatg agccgctcct tcagttcggc cagtttctcc tgggagcgcc ccatcaggcc
     2461 tactttgtat ccgctgcggc atagtctttc cgccagcgca ctgccggtgc agcctgtcgc
     2521 tcccatgatc attactgttt ttttgtccat ctttcacgcc tcgtcttccc tattctgagg
     2581 ttaggcaaag cagatgaata aggtgtgaac tcgtaagcaa gggagttcgc gaccgctcac
     2641 ctggtggttg ggaacgtctc tattttcacg tgtatttcac tttcgatgag ctagtttggc
     2701 attatctcgt gccaagataa gtcgactcga tcggcttatc ttcttttggc ataatactgc
     2761 tggtggtggc acaagtggtg ccgctagcag agctgcgcac cggcatgaac aggcattctt
     2821 ctgccgaaac gccgaaagat aatagacagg cattgcttct gacggtttgc tctttatata
     2881 aataaattac caattcactc ttctaaaaac acgaggctaa tctagaaaga ctaccccgcc
     2941 cacagtttga gcaaaaagtt tgagccggtc gggtcaaaga gtgaatgcaa gtctggaatt
     3001 taagtaaaga cgcaaaaagc agtcaaacta gaagctgaag agccttaaat caggctccat
     3061 caagggaaca tcccatgaca aagacagata tcactgagtc ggccgctatt caacagaggc
     3121 tggctgaaat gcaggctcaa gccgtgagtc agtcggagat cgctctgcac tgggccggcc
     3181 ataaccgaca ggatctggct atcgtcttca tcaacatggc taagctcatg ttgctcgagg
     3241 gtgaagggct tggaagcgag agtgagttct gggttttgac caaccgtgcc gccgttgccg
     3301 agtttgcccg gcaacccaag cttgccctcg acttagccgt tcgcgctcac gatctgggcg
     3361 agaagctctt cctgggcgac gccctcaaac tggcggtggc acgcggcaat ctgggcaaag
     3421 ctctggccgg cgccgatcgc ccggaggagg cacgcaaaag gctgagcgaa ggcctctttg
     3481 gggtctccac cgccagccat agcccgcctg cccacacccc ggagccgctc aagaagtatt
     3541 atcgcgaagc tttcatggac tatgctcgcg ccctgcagaa gttgccggca agaccgcggt
     3601 aaagcctgtt caggcagcgc tttctcagca acacatgctg accatctcgt tctcaccatc
     3661 actcatcaaa gggctccggt cgggtaaccg accggagact ggcccatgtc ttttgcaagc
     3721 atgtcggact ccctgagcgg gctcttaccg ttctatgtgt ttgttgccac agctgcctgg
     3781 ggtctctggg gcattttcga caaactggct ctggagcgtt cgcaaccaaa ggacgtcttc
     3841 cttgccacca cgctttttgc cctgcctcag attccacttt tgctcttcat gctctactgg
     3901 agtcaacctc agtggcagct cacgccgtcg ctcctgattt acgccgggct ttcttctctg
     3961 gtctcatctc tgggtatgat gctctacctg gactctatga agcgcaccga taccacggtg
     4021 atactgagcc ttaccgccgc ctatccgctg gtgactcaga tcgtctgctt cttcttcctg
     4081 gaagagacat tctcggctgg acgggctctg ggtgccgtct tcattgcctc cggtgtggta
     4141 gtgattggac tcacggacaa ttccagtcct cagcccatca ccaatcttcc ccagccgggt
     4201 aagcccgatc cttcatccaa tcacgagcca gatccttcat cagagcagaa gccggagcgc
     4261 cttccagtcc ttagatctgc cctgagatta tttctcagac caggccttgg acgtcttttc
     4321 ctgtcggcaa tggtggtgaa tgtttgctgg ggtctcaagg gagtcttcga gaaattgtcg
     4381 gtgcaacacg gcacacctct ggagtcctat ttctcggaat gtctgaccaa tctggcacta
     4441 cttctgccgg ttctgctctg gtttttctgg agcggtttca aacccaggct gaagagcaaa
     4501 catctctggc gttacaccgg cctttccgaa ctggcgctgg ccgtcggcgg tatctcttat
     4561 ctgacggctc tcagcctggc accggcaagc tatgtggtga cgatcacgag cacctatcca
     4621 ctgctcatgt atttcatcgc cttgtttgtt ttgaacgaaa agttcaatcg catcagactc
     4681 ttcggcattg tctgcatagt tgtcggcggg ctgattgtct agatggccaa gtgctgactt
     4741 tctaaatggc ggcggacagg ttgaaacggt gcccgtcggg catacgcttc actgtccgcc
     4801 cgggacggat gagctgtgtg ctgctagttt gcgggttaac ttctgcatag ctgatacttt
     4861 cttctatgtg aaaagaattg cccatgagta attgtcccct gagtcagtgc tgcttagcgg
     4921 cccacttgcc tatgctgttt ataaatgtcc ctacatcccc atcttcagga tatatcaact
     4981 gctgggtcga agtgggcgaa attgagtcaa gtgctatatc caaccacttt cgcttgctag
     5041 tatcctgcag attgcaccgg gagagccaac tctcaagctg acctacaggt acaacaaaaa
     5101 ttccccaatt cgccaattct gagatgtaag aattgaaaac ttcatacatt tcattattca
     5161 ggacatctcg gggacatttt tttagcttct ttttgtcttc ctggttgact tgattcctga
     5221 gttccttgcg cagattctca agcctgtcta cggcgcctgt agccattcct aacttttgat
     5281 atgttctttt gtgttcagca gcatcatcaa aaagatagtc gacatcgact atgccagcgg
     5341 ctggaagccc aaattctctg aggttctcta ccatgcttac taccatattc ttacttggag
     5401 cctgcgtaaa atgtgcatcc gcgattcctt gactctgg
//
LOCUS       DAOQNZ010000050         5432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6069_length_5432_cov_3.585271, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000050 DAOQNZ010000000
VERSION     DAOQNZ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6069_length_5432_cov_3.585271"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1456)
                     /locus_tag="PKW73_01550"
     CDS             complement(125..1456)
                     /locus_tag="PKW73_01550"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNA71993.1"
                     /translation="MNTDAKQTKSEQELSELEDSDIHQTKSKEQRSHMHDNESFELIA
                     LPQRKPIKAWVKGVPLEEEARRQLINLASMPFIHSHIAVMPDAHMGKGTTVGSVIPTT
                     KAIIPAAVGVDLGCGMIAVKTDMKARDLPDDLKAVRKAIEDTIPVGFACWKDNMASSH
                     PAEKKINKAWAGLTDRFDAIVEKHPHLDNGSLRCRLQIGTLGTGNHFIEMLTDDLGFV
                     WLMLHSGSRGVGNRIGTYFIDLARKDMQKHHIHLPDRDLAYLSEGTEYFADYLEAVSW
                     AQDYAYINRQVMLSLLMEALKKQKSIPAFQADLKVINCHHNYVAREVHFGENVWVTRK
                     GAVRAQEGDWGIIPGSMGTGSFIVRGKGNAESFNSCSHGAGRTMSRAKAKQTISLDKH
                     KRDTAHVECRKDVGILDESPSAYKSLDAVMKAQADLVDIVHRLKPLVCVKG"
     gene            complement(1463..1795)
                     /locus_tag="PKW73_01555"
     CDS             complement(1463..1795)
                     /locus_tag="PKW73_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71994.1"
                     /translation="MRYVIEAQSLYNLLQQVRLERVFVVAGNREYGPCNREKRMETIA
                     VIDEINGELDKEQDSAVTDRAVHRFTPVTASGPGSYGNAVQSFCRAWRDSTGIKVEIY
                     NPEDQQVR"
     gene            3230..4312
                     /locus_tag="PKW73_01560"
     CDS             3230..4312
                     /locus_tag="PKW73_01560"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA71995.1"
                     /translation="MAQAEDGSGSLYRRPHEWKMKPLAGGSSSRRSQTAALSASPNSF
                     SSNSAPSASPVTSFPVSRSGASFSSLSSRSSARLSEPALSAGSALKSWPVPSPRAVIL
                     SLHGFGLSKEAFDSFARAMNARGITCYAMDLRGFGSYIKNSDGAGGNADASKIDFYQT
                     ILDVKMSLAWLRQSHGRVPLFLLGESMGGAVALQTTSMFPDAVEGVIVSVPGDSYYKT
                     FEAASRVALEVLKPGGGIDLSDHVVGHSTSDPNLRKDWLTDPSARLSVSPAELLAFRR
                     FMSRTDELARLINKTPVLMFHGFQDKLSKPDGTLKIFREVGSRDKDLVMLGRAEHLIF
                     EKEQFDAHIVELVRSWIDKHSASVSL"
     gene            complement(4343..5389)
                     /locus_tag="PKW73_01565"
     CDS             complement(4343..5389)
                     /locus_tag="PKW73_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA71996.1"
                     /translation="MTYDTDASESVMSDELALDAIASSMVLFDGAAGISPVTDLFSEE
                     AEISWVEETPRAKAGRPKGSSVSDLEKESGASEPAFYAYLQNISKNSLLKAEDEIELG
                     RKISKGDRDALAKLVTSNLRLVVSIAKRFRNQGLDMEDMVQEGNIGLIHAARKFDPSM
                     GNRFSTYATWWIRQAIMRAIANKGRTIRLPVHVRGQLTKLRKCARDYHQKLGRFPTEQ
                     ELAQETGIEAAEVQRLLSGISGLMSLDDAIAGSDKEPLGSFIEDTESPRPETEAEASM
                     LRRVIDKLTKHLTPLESKTVSYLYGLEDGIACDTRMVANILKLDVQEVRRIQKRCLKR
                     MRRHLYNRSIEDFV"
BASE COUNT         1144 a         1405 c         1359 g         1524 t
ORIGIN      
        1 cgtcaaagtt ctcaccgcac cggcgccgga atccaccagg agccagtttc aagacctgag
       61 agtgagtatg ataactgagc cggtgggcat cagcggaagg ctgcgctagc accggctcaa
      121 ttatttatcc cttcacgcag accaggggct tcagcctgtg aacaatgtcc acaagatcag
      181 cctgggcttt catcaccgca tccagtgatt tataggctga aggcgattca tccagaattc
      241 ccacgtcttt gcggcattcc acgtgagcgg tgtcacgttt gtgcttgtcg agtgagatgg
      301 tctgtttggc cttggccctg gacatggttc ttccagcccc atgggagcag ctattgaagc
      361 tttcggcatt gcctttaccg cgaacgataa aggaaccggt acccatagaa ccgggaatga
      421 ttccccagtc cccttcctga gcgcgcactg cacctttcct ggttacccat acgttttcac
      481 cgaagtgcac ttctctggcg acatagttgt ggtggcagtt gatcaccttc agatcagcct
      541 ggaatgccgg aatggatttc tgctttttga gcgcttccat aagcaagctc agcattactt
      601 gcctgttgat gtaggcgtaa tcttgagccc agcttactgc ttccaggtag tctgcgaagt
      661 actccgttcc ctcagaaaga taggcaagat cgcgatccgg taagtggatg tgatgcttct
      721 gcatatcttt tctggcgaga tcgatgaagt atgtaccgat acggttacct acgcccctgc
      781 tgccggagtg cagcataagc cagacaaagc cgagatcgtc ggtgagcatt tcgatgaagt
      841 gattgccggt tcccagtgtg ccgatttgca atctgcaacg cagggagccg ttgtcgaggt
      901 gcgggtgctt ctcgacaatg gcatcgaagc ggtcagtcag accggcccag gccttgttga
      961 ttttcttctc ggcaggatgg ctggatgcca tattgtcttt ccagcaagca aagccaaccg
     1021 ggatggtgtc ttcgattgcc tttcttacag ccttgagatc atcaggaagg tcacgggctt
     1081 tcatatcggt tttaaccgca atcataccgc agccaagatc gacccccact gcggcgggaa
     1141 taatcgcctt ggtggtggga atgacactac cgacagtggt acccttaccc atatgagcat
     1201 cgggcatgac ggcaatatgg ctgtggatga aaggcataga ggccaggtta attagttgcc
     1261 gcctggcttc ttcttccagg ggcactcctt ttacccaggc tttgatgggt tttctttgag
     1321 gaagtgcaat taactcaaag ctttcattgt catgcatatg agatctctgt tctttcgatt
     1381 tagtctggtg aatatccgaa tcttccaatt cagataattc ttgctctgat tttgtttgtt
     1441 tagcatcagt gttcattttc cattatctca cttgttgatc ttcgggattg tagatttcta
     1501 cttttatgcc ggtactgtct ctccaggcac ggcagaagct ctggacggcg ttaccgtacg
     1561 agccgggacc ggaagccgtc accggtgtaa atcggtgaac ggctctgtcg gtcacggcag
     1621 agtcttgctc cttatcgagc tcgccgttaa tttcatcaat cactgcaatg gtttccattc
     1681 gtttttccct gttgcaagga ccatattcgc ggttaccggc aacgacgaag actctttcga
     1741 gcctgacttg ctgcaacaag ttgtagagtg attgtgcttc gattacgtac ctcatgatta
     1801 acctcctcat aaaggtttga actcggaaaa aagcttttgc ttcggattat ttgagcgcga
     1861 caaggcgccg caccggatgt ggtgcgtgtt atcgcggtgg taccaatcga ctcccgaccg
     1921 gcacacgcct ttgaagaaat ggtgaagagc tttaattgaa ggtctaagcc gtgaacggca
     1981 ctaccctcaa ggcgcttctc tttctttctc tatggggagc agtaaccgtc ttgcactgac
     2041 gcacttgcat ccatgtatat tttcttgtgg cagcctgcgg tgccgctaac aacaaagtta
     2101 tttcacttga gcctcaggta aaccttttgc tcaggtcttg ttgcttagaa ctatgctgtt
     2161 tgcaaaagtc gctgcaattg gctaactgca aatggcttat agcttactgc gccgggccgg
     2221 ctttgccggt attggctggc gtacaagatt ggtctggctt gaactgccgt gtgtcaggct
     2281 tctaagaaca gaccggcact tattgcttaa ctcagccagg gttttgccgg gagcaaaggc
     2341 ggacccatca cggccacttg cggcgaccgt cgacataaag ttgcgcattt ctgccgcata
     2401 gttttttgtc gtgtagatgt aagacatagt cttgccctcg ctatagatct tcaggataag
     2461 ccagcaatcc ttgctttgtc aaggtggtaa atccctcatt ttgcctttgc ttttcaatca
     2521 gtggcaggcg atttgagcga tgcaccgctg gtggtgttta gtcgaaggcg ccggggaggt
     2581 cacttcaaag cacctgtttt tttgcttcct tagcctgtag agagagcttc tgcttgggtt
     2641 gctatccgta gtcaaagaaa aggtcgctat cttttgacag cgacctgaaa ctttttaggt
     2701 tgttatttca attccggttt tcttcaccgg atgagttttt agcaaaaact ttttgactag
     2761 tggaaaccct cttcttcgaa ctccccaccg aagggcaggt agtcatttat taatgcgctc
     2821 aaaattgtgc ctacgagaat tacagctacg ctaagcaatg tcataaaaaa ttgacgactc
     2881 caagggaatt cagcaaatta tccgccttaa agtatatgca aagagcatat ggtgaagttt
     2941 cgttacggat ttataatttt ccccggattc ggcaattcca ttagaataaa atttgcgctg
     3001 cctcttggca ggattggttt tctggtctat gctgagcctt tgtcggaact tgttcgaatc
     3061 ttttgagcct cgctcagccg tgtcctgata aggtcctttc aaagccccct tttggggttt
     3121 ttgcctcctt agtaattcca ttaatagaac cggtcgacct gtgaaaacat ttcgctgtcc
     3181 tagccttcta tttgtctggc ttctcggctt gttgtggctg ggcgcttcca tggctcaagc
     3241 cgaggacggc tccggttcct tatatagaag accccatgaa tggaaaatga agcctctagc
     3301 cggtggctct tcctcccgtc gcagtcagac ggctgccctc tctgcttctc caaattcttt
     3361 ctcgtctaac tccgctccgt ccgcttcgcc ggttaccagt tttcccgtat ctcgttcggg
     3421 cgcttccttc tcttccctct cttcccgctc ttccgctcgt ttatccgagc ccgctctctc
     3481 tgccggttcg gctctcaaga gctggcctgt gccgtctcct cgggcggtaa tcttgtcctt
     3541 gcacggcttt gggttaagta aggaggcttt tgatagtttc gccagagcta tgaatgccag
     3601 gggaattacc tgctatgcca tggacctgag gggatttggc agctacataa aaaattcgga
     3661 cggagccggc ggcaatgccg atgctagcaa aatcgatttc taccagacca tattggatgt
     3721 gaaaatgtcc ctggcctggc tccgtcagag tcatggcaga gtgccgctct ttttgctggg
     3781 tgaatccatg ggcggggcgg ttgccctgca aaccacctcc atgtttcctg atgcggtgga
     3841 aggtgtgatc gtctcagttc cgggagattc ctattacaag acttttgaag ccgccagccg
     3901 ggttgctctt gaggtcctca agccgggtgg aggaattgat ctttccgacc acgtagttgg
     3961 gcactccacc agtgatccca acctgaggaa agactggctg actgatccgt ctgcccgttt
     4021 gagcgttagc ccggccgaac tcctggcgtt caggcgcttc atgagtcgaa ccgatgaatt
     4081 ggcacggctt atcaataaga ctccggttct aatgtttcac gggtttcagg acaaattgtc
     4141 caagccggat ggcactctga agatatttcg ggaagtgggc agccgtgaca aagacctggt
     4201 tatgctgggc cgagccgaac atctgatttt cgaaaaggaa cagtttgacg cccatatagt
     4261 tgagcttgtg agaagctgga tcgacaagca ttccgccagc gtctccctct aggcaagagg
     4321 tttgcaccca gaagagcagg ctctaaacga agtcttcaat agagcggtta tagaggtgcc
     4381 ttctcatgcg tttcaaacag cgcttttgaa tgcgccgcac ttcctgcaca tctagcttca
     4441 gtatgtttgc taccattctg gtgtcgcaag caatgccgtc ttccagacca tacaagtaac
     4501 taacggtttt gctctccagg ggtgtcagat gcttggtcag cttgtcgata acccggcgaa
     4561 gcatgctggc ttccgcctcg gtctccggtc ttggtgattc ggtatcctca atgaagctcc
     4621 ctagcggctc tttgtcactg ccggcaatgg catcatcaag cgacatcagt ccggaaatgc
     4681 cgcttaacag ccgctgcacc tccgccgcct caattcctgt ttcttgggcc agttcttgct
     4741 ctgtggggaa gcgccccagc ttttgatggt aatctctggc gcacttcctt agtttggtta
     4801 actgtcctcg cacatgcacg ggcaggcgaa tggttctacc tttgttggct attgctctca
     4861 tgatagcctg ccttatccac caggtcgcgt atgtggagaa ccgattaccc atgctgggat
     4921 cgaacttgcg ggcggcgtga atgagaccga tattgccctc ctgcaccatg tcttccatgt
     4981 cgagaccttg gttgcggaag cgtttggcaa tactgaccac gagcctcaag ttactcgtca
     5041 cgagtttcgc cagcgcatct ctatcgcctt tggaaatttt gcgaccgagc tctatttcat
     5101 cctcggcctt gagtagactg ttcttgctta tattctgaag ataggcatag aaagccggtt
     5161 cagatgcacc ggattccttc tcgaggtcgg agaccgaaga acccttagga cgtccagcct
     5221 tagccctggg tgtttcttct acccaactga tttctgcctc ctctgagaac aagtctgtta
     5281 ccggactgat gccggcggct ccgtcgaaaa gaaccatcga ggaagctatg gcatctagag
     5341 caagttcgtc gctcatgaca gactcacttg catctgtatc gtaagtcatt tcgtcaatca
     5401 tcgtgctcac cgtgtttcct ttttacactc ca
//
LOCUS       DAOQNZ010000051         5422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6090_length_5422_cov_3.373207, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000051 DAOQNZ010000000
VERSION     DAOQNZ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6090_length_5422_cov_3.373207"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PKW73_01570"
     CDS             <1..340
                     /locus_tag="PKW73_01570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA71997.1"
                     /translation="PLAYIQSYIACLCSEGWWEDITDPATVGIDFFIGEAGKLNHGLG
                     TALLQAFARKLFSNPSVNRIISDPSPENPRSIAALQKAGFTRLGDISTPDGAAVLLEL
                     KRSAATSNVL"
     gene            complement(327..1223)
                     /locus_tag="PKW73_01575"
     CDS             complement(327..1223)
                     /locus_tag="PKW73_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNA71998.1"
                     /translation="MPGEKYLENVMAAIDFIEEHLLDENLSASEAAKHAGFSEYHFHR
                     IFTSLTGDSIAEYIRDRRLSEAALELGQSRESILTYAIKYGFESQETFTRAFKRTFGV
                     TPGAYRKARQGRSLPPDMMAKRAFSLALLEHICKGGINMEPVIVERQEEYVVGMGDSF
                     APKASIKIKALWDEFMKRSHEIEARSDLAYGVCTAKHPEIETKEGCCMVYIAALPVQE
                     DSEVPPGMVKVKLEGGEYARFTHRGPILEIGRTVDYIWGQWNPQAPYKRRNSADFELY
                     DERFNPESAESEVDIYVPLTRR"
     gene            complement(1372..3123)
                     /locus_tag="PKW73_01580"
     CDS             complement(1372..3123)
                     /locus_tag="PKW73_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA71999.1"
                     /translation="MKLTRRFYLYFSLAICLVLLIAPLDYWLKHPLLTYADQALYLEC
                     ARLILEGQVPYRDFFEWNPPLIMYLSIVPVVLAQILKLPIILCFNLCVLSLSALSAAL
                     SLFLLNRFLTIRGFISMVPVVLSAVYYSCRELYSFGEREHLFVLAYFPFFIWRMLRHQ
                     GKHLAALEGYLPLPLGVWTGLMLCLKPQFVMCAAACELVFLMGKAKSTLVKRMKDPEI
                     LVFLSIAGLYLLSLLITPGGAAQTLFMKILPMHGQGYDFSRRALIYMLRGDNFMATAT
                     YTILAGSALGFLFGRYSLYISALGAFLSIGLFNYIYGGQAWIYRLVPAEFAAFLIVAG
                     GVGLLLRLAILKKKQIQTISLLVLTLLMGLSLYLTQGAMAATRLTLATSEPFDLARVG
                     YNGSCPRGDFEGLFYAIVDNTSAGDRVAFLGTGISPGFPAILQANRNSGAHYLFSILA
                     QIQHCRNLYGEEKWQPMLDKLIEKHGLDVQKFSPKLIAIQAIHMNAILRRANFFERYM
                     LNYKKIGTVDGNDLYLRVGAGGRNLSPQAWKALHKEVVLNILGEKATVPDESRRTGLD
                     EELIKRWIEEANQMLSK"
     gene            complement(3120..4835)
                     /locus_tag="PKW73_01585"
     CDS             complement(3120..4835)
                     /locus_tag="PKW73_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72000.1"
                     /translation="MRKLLPNFNFKAEASLFCTLLLCAPSLVLLSADLLYHPINISSW
                     LAMRIYAARLMTEGKELYLDFFDWTQPLLLGTFKLLLQARELLVWLWTGLQAIGQNSI
                     KESLPYFLLPDIYITLVILTALAMSLLIGARLAATGSKDGQHRHWLLLPVALSLTAYV
                     VRFDFGDLQFLLLLSLVPWLLLKLKRAAGETVNTPLALFIGTLAGLAACLDLHYILVF
                     AVLSFLFKPVKKKPAPETLTLLLIPVLYLAWLFALPAEQSKIFYQWAMPVRLLQFTTP
                     NADLFGPDSCAHRFDVWCASALALFTAQALSAKTSFLQTAGKQTLRSLGVLLSAGLAL
                     YVIENQGLSKDLILAIFAATSIFLVALAETSRELVSRFSKEEREALPLKYAVIAALLL
                     ISMATLAIGENLRQDRQLLLHAQTGGIKSEKVDLETALTTYSRPGSKIVFFNELAQPA
                     YPMLLTYERKPGSYLLFSRPLLLLNWHKEHGKLTDALSEFHNYIHDDLKAALESKDTA
                     LVIMDATMLPTMKSSGLEKTISDGFKQLPPGCFYSARNRQPHEYAGYNWEYFIFGRNS
                     GETSQ"
     gene            5048..>5422
                     /locus_tag="PKW73_01590"
     CDS             5048..>5422
                     /locus_tag="PKW73_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72001.1"
                     /translation="MVETRVLASKPGLRLVFSSWLLAWCGLLSVFLPSRGNTLFAAES
                     DSSWPESSLARRQFLGEIEASDLDVLLRGAAERDAGEIAARLKRVCPEALGEVKDIFH
                     GVELCGHFPLPAGKDESEGDRAE"
BASE COUNT         1259 a         1343 c         1464 g         1356 t
ORIGIN      
        1 gcctttggcc tatattcaaa gttatatagc ctgtctttgt tcagagggct ggtgggaaga
       61 cattaccgat ccagccaccg taggcatcga cttctttatc ggtgaggccg gcaagctcaa
      121 tcacggtctt ggtacggcat tgctgcaagc ctttgcccgc aagctattct cgaatccttc
      181 cgtgaacagg ataatatccg acccttcacc ggagaatccg cgctctattg ccgccctgca
      241 gaaagccggt ttcactcgcc tcggcgatat ttctactcct gacggcgcgg cggtcttgct
      301 ggagctgaag cgatcggcag caacttctaa cgtcttgtaa gtggtacata aatatccact
      361 tcactctcgg cgctctccgg attgaagcgc tcgtcgtaaa gctcgaagtc ggcgctgttc
      421 cgtcttttgt aaggggcctg cgggttccac tgtccccaaa tgtagtccac ggtgcgaccg
      481 atctccagta tcgggccccg gtgggtgaat ctggcgtatt ctcccccttc cagtttgact
      541 ttcaccattc ccggtggcac ctcgctgtct tcttgcaccg gtagagctgc tatatagacc
      601 atgcagcaac cttctttcgt ctctatttcc gggtgcttgg ccgtgcagac accgtaagcc
      661 agatctgacc tggcttcaat ttcatgagag cgcttcataa actcatccca gagtgctttg
      721 atttttatgg aagctttggg cgcgaagctg tcgcccatgc ccacgacata ctcttcctgc
      781 cgctccacta tcaccggttc catgttgatt cctcctttgc atatgtgttc cagaagggcc
      841 aggctgaagg ctcgctttgc catcatatcc ggtggcaggc tccggccttg ccgggctttg
      901 cgataggctc ccggtgtaac tccgaaagtt cgtttgaaag ccctggtaaa tgtttcctgg
      961 ctttcgaatc catatttgat tgcgtaagtg agaattgact ctcggctttg cccaagctct
     1021 aaggctgctt ctgatagacg cctgtcgcga atatactcgg cgatggagtc gccggttaag
     1081 gaagtaaaga tacgatggaa gtggtattcc gaaaatccag cgtgctttgc tgcctcgctg
     1141 gccgaaagat tttcgtcgag gagatgctcc tcgatgaagt cgatggccgc cattacattt
     1201 tcaaggtatt tttcgccagg cacgtagata tcttaagtga agtgaaaatt gcggttttga
     1261 ccttttttgc taagttggct ccattgattt tctagttaga attctatgtc aagggcttga
     1321 ccgcttttgc catagtcgta gttgcctgcg acaaaggcac gtggcggtat tctatttact
     1381 tagcatttga ttggcttctt ctatccatct tttaattagt tcttcatcca gtcctgttcg
     1441 tctgctctca tccggcacgg tggctttttc acccagtata ttcagcacca cttctttgtg
     1501 cagtgctttc catgcttgcg gcgatagatt gcgaccgccc gcacccaccc gcagataaag
     1561 atcattaccg tcgactgtgc caatcttctt atagttgagc atataccgct caaagaaatt
     1621 agctcgcctg agaatggcat tcatatgaat cgcttgtatg gctatcagtt ttggtgaaaa
     1681 cttttgcacg tcaagaccat gtttctcaat taatttatcc agcatgggct gccatttttc
     1741 ttctccgtag agattgcgac aatgttgtat ctgagccagt atggaaaaga gatagtgggc
     1801 tccggaattt ctatttgcct gcaaaatggc ggggaagccc gggctgatgc cggtgcccaa
     1861 aaaagcgacc cggtcaccgg cactggtatt atcaacgatg gcatagaaca gcccttcgaa
     1921 atcgcctctg gggcagctgc cgttgtagcc gacgcgggct aagtcaaagg gctccgatgt
     1981 ggccagggtc agcctggtgg cggccatggc gccttgcgtc agatagaggg acaaccccat
     2041 tagtaaggtc agtaccagca aagaaatggt ttggatttgt ttctttttga gaatggctag
     2101 cctcagcaat agtccgacgc cgccggcaac aatcaaaaag gccgcgaatt cagccggaac
     2161 tagccggtag atccaggcct gtccgccgta gatataattg aacaagccta ttgataaaaa
     2221 ggctcccaga gctgaaatgt agaggctgta gcgaccaaat agaaagccca gggcgctgcc
     2281 tgccagtatc gtataggtag ccgtagccat aaagttatcg ccgcgcagca tgtagattag
     2341 cgctcgtctg gaaaagtcgt agccttgccc gtgcataggc agaatcttca tgaaaagagt
     2401 ctgtgccgca ccgcccggag tgattagcag cgagagcaaa tatagaccgg ctattgagag
     2461 aaagaccagg atctcgggat ctttcatgcg tttgacgagg gtgctcttag cctttcccat
     2521 tagaaagacc agctcacagg ccgcggcaca catgacaaac tgcggtttca ggcagagcat
     2581 caggccggtc cagactccca gcggcagtgg caggtagcct tccagcgcgg ccaggtgttt
     2641 gccctggtgg cgcagcattc gccagatgaa aaagggaaag tacgccagca cgaaaagatg
     2701 ctcgcgctct ccgaaggagt aaagttcccg gcaggagtaa tacaccgccg aaagtaccac
     2761 cggcaccatg ctgataaagc ccctgattgt cagaaagcga tttaaaagga ataaggaaag
     2821 ggcggcagag agggcactga ggctcaggac gcagaggttg aaacatagaa tgatcggtag
     2881 ttttaagatc tgcgccagca ccaccggcac aatactcagg tacattatca aaggtgggtt
     2941 ccattcgaag aaatctcgat atggtacttg tccttccaga atcaacctgg cacactccag
     3001 gtagagggcc tgatcggcgt aggtcaagag tgggtgtttg agccagtagt cgagtggtgc
     3061 tatgaggagc accaggcaaa tggcaagact gaaataaaga tagaagcggc gcgtcagttt
     3121 cattgggacg tttcaccact gtttctaccg aagatgaaat actcccagtt gtagccggcg
     3181 tattcgtggg gctgtctgtt gcgggcgctg tagaagcagc ccggcggcag ttgcttgaag
     3241 ccgtctgaaa tagtcttttc cagtcccgat gacttcatgg tgggtagcat ggtcgcgtcc
     3301 atgattacca gtgccgtatc cttgctttcg agcgctgctt tcaagtcgtc atgtatgtag
     3361 ttgtggaatt cactgagtgc gtccgtcaat ttcccgtgtt ctttatgcca gttcaatagc
     3421 aggagggggc ggctgaataa caggtagctg cccggctttc tctcgtaggt caaaagcatt
     3481 ggataggccg gttgggccag ctcattgaag aagactatct tgctgcctgg tctggagtaa
     3541 gtggtaaggg ctgtctccag atcgactttc tcactcttga ttcccccggt ctgggcatgt
     3601 aacaacagct gccggtcttg cctgaggttt tcgcctatgg ccagggtggc cattgaaatc
     3661 agaagcagag cagctattac agcatacttg agcggaagcg cttctcgctc ttctttgctg
     3721 aatcgactca ccagctccct gcttgtttct gctaaagcaa ccaggaaaat actcgtggcc
     3781 gcaaagattg ccaaaatcag atctttcgat agtccctggt tttcaatcac gtaaagagcc
     3841 agtcctgccg aaagcagaac ccccagcgaa cgcaaggttt gtttgccggc cgtctgcaag
     3901 aaggaagtct tggccgaaag ggcctgggct gtaaacaggg ctagagcact ggcgcaccag
     3961 acatcgaagc ggtgcgcgca ggaatccggt ccgaagaggt cggcgtttgg ggtagtgaat
     4021 tgcaataatc tgacgggcat tgcccactga tagaatatct tgctttgttc ggccggcaaa
     4081 gcaaaaagcc acgccagata gagcactggt atcagaagta gtgtcagagt ttccggtgcc
     4141 ggtttcttct tgacgggctt gaaaagaaac gataggacgg caaagaccag gatgtaatgc
     4201 aggtcgaggc aggctgccag accggccaga gtgccaatga acagagccag gggggtgttt
     4261 acggtctcgc ctgccgcccg ctttaacttt agcaacaacc agggcaccag gctcagtaga
     4321 agcagaaact gcaagtcgcc gaagtcgaat ctaaccacat aagctgttag cgaaagagcc
     4381 acgggcagaa gcagccagtg cctgtgctga ccatctttgc tccctgtggc tgccagtctg
     4441 gcgccaatta gaaggctcat tgccagagcg gttaagatga ccagggtgat atatatgtca
     4501 ggcaaaagga agtaggggag actctccttt atgctgtttt gtccgattgc ctgaagacca
     4561 gtccagagcc atacaagaag ctctcttgcc tgcagcaata gtttaaaagt gccgagaagc
     4621 aggggttggg tccagtcaaa aaagtccagg tataattctt tgccttccgt catcaacctg
     4681 gcggcataaa tgcgcatggc aagccagctg ctgatattga tcggatgata tagaagatct
     4741 gcacttagca gtaccagaga tggtgcacaa agcagtaagg tgcagaacag gcttgcctct
     4801 gctttgaaat tgaagtttgg cagtaacttc ctcatcaccc tcgcattcca ctttcaactt
     4861 cctgccgccc atcacggcga gtaaataagg gtaacggtat tgggctatat ttgccagtga
     4921 aatttggcag acttagagct ttcaattgaa cttggcgact cctgttgaag ctttcttgcc
     4981 caggtctcag ccatgaatta aactgaggga agggtcagcc acatttgctc tcatggaaga
     5041 ggagattatg gtagagacgc gggttcttgc gagcaagcca ggtcttcggc tggtattcag
     5101 ttcctggctt ctggcctggt gtggtcttct ctccgtcttc ttgccgtccc ggggtaatac
     5161 tctctttgct gccgagtcgg atagctcatg gcccgaaagt agtcttgccc gcaggcagtt
     5221 tctcggagag attgaggctt ccgacttaga tgttctgctt cgcggtgccg cggagagaga
     5281 tgccggagag attgctgcca ggctcaaaag ggtatgtcct gaggctctgg gggaagtgaa
     5341 ggatattttt cacggtgtcg agctttgtgg gcattttcct ttgccggcgg ggaaggatga
     5401 gtctgagggc gatcgggctg aa
//
LOCUS       DAOQNZ010000052         5417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6108_length_5417_cov_4.586535, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000052 DAOQNZ010000000
VERSION     DAOQNZ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6108_length_5417_cov_4.586535"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKW73_01595"
     CDS             <1..204
                     /locus_tag="PKW73_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72002.1"
                     /translation="ARQKKAEAEAAAEAARRKAEEEKKKAEEERIRKAEELKREQQRE
                     EERKRKEAVVDKELEELKKKLGK"
     gene            complement(248..2245)
                     /locus_tag="PKW73_01600"
     CDS             complement(248..2245)
                     /locus_tag="PKW73_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HNA72003.1"
                     /translation="MSAESSGIRETSKATELETQPLSPLQGQYLAALSERQAAARKDS
                     EREPVCPVPDGKTGKLAGAIGSGLLGFMGEQPNSSPGSNSSKSGHSDASNESKTISSA
                     AAGASFFYGGRSFFKPDSSVLRNGDGSVASINFKDGLVGSFEYFPDKNLRSVKLSDGR
                     VYTRADAKAQWLDQNSAPADVTKIEVDGGRLRLTDRTAVTTHMSSSESVRKEFPNFQP
                     DKLAGLYLKNESRLDLDKNGSIDRKELARGIVSNDFKGEDAQFVAALQLRYADLKGQR
                     TGFLGWDDNGITLTDLQDFSAKARSEKALSDSAKNYEDVVTNKLLSEPGKSVTEEEVR
                     AAVASTSDAAERDRLAQFLADFDKFTVSDWNPLAKKRVDLSSFQKGLKTLADAPAYAA
                     ARTMEGWVNSGSDSLEKGTESVFGDERIGGGGGIRAAAVRQGSVGNCYFMAGLASLAE
                     ASPQTIKNMITSNADGTFTVRFPAIPDEPFVVSRPTEAERMLYSRSSQSGIWPQLIEK
                     AYGAYRIKHGLVKPEARDRETGIAQEGSDGGDTIANGLSLLATGEVAVIEIAGKSEQE
                     IGASISASLKAGKPVSLGLHKDEAETQKAGSPLEGLSNNHAYSVIGYDERSGKVLVRN
                     PWGSGEPSKNGIARDCDNDGVFALTVGELKNYFYNMAFFKN"
     gene            complement(2554..3006)
                     /locus_tag="PKW73_01605"
     CDS             complement(2554..3006)
                     /locus_tag="PKW73_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA72004.1"
                     /translation="MIKTETEYQECLKRLESDSRVIQEQRRKLEELKLSSEQIDLAME
                     PVLSFNAQLKEEVEWYERVKRRDFGVFTQLTEIGPLLVALRIANGISQSELARRLSVD
                     VSQISRDERNDYHGISIERAERVVAALGEELQISVRYKKQPGDLMVAS"
     gene            complement(2999..3472)
                     /locus_tag="PKW73_01610"
     CDS             complement(2999..3472)
                     /locus_tag="PKW73_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72005.1"
                     /translation="MVPSEGSSKDWDAIWRARLVDNLGVMTNQLWMVGVTNIFIDGSF
                     VEEKDHPNDIDGYFECELAYLASGALQQDLNKLDPNKVWTWAPASRTPYANSTKKQLP
                     MWHFYRVELYPHFGQSSGIKDLFGNDLQFPAAFRKSRTEHRQKGIIKLLQERASD"
     gene            4178..4525
                     /locus_tag="PKW73_01615"
     CDS             4178..4525
                     /locus_tag="PKW73_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72006.1"
                     /translation="MRTVAGALLLLSAAALVFGRHSESQIPFDSAIWKHQVNSSERLR
                     MVGDLKTKLMSSGREEIEQILGEPRRGFDSLHPNDYYYLLGTQERLLDTDGIWLRITF
                     QNDRVCDMQVIHD"
     gene            complement(4678..>5417)
                     /locus_tag="PKW73_01620"
     CDS             complement(4678..>5417)
                     /locus_tag="PKW73_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA72007.1"
                     /translation="QWAQSVEADRQDVNSLMKRVNAKDHELSMRKEELAAKIRGYESF
                     SDFASYLEIPAPFNGYVTERRMHVGSFVGPDGTGAYPPICRIKQLDLLRIVAPVPEID
                     TAGVVVGSEVQFTISAFPGRRFVGTVARISNNLDKATRTMPVELNFLNPKYEVLPGMF
                     CKVYWPTRRRENSLFVPVSAVISTPLDTYVCKVSDGRVEWVSVRKGQIMGDQVEVFGG
                     LKEGDLVAREGNEELQNRSLVTPVRAK"
BASE COUNT         1428 a         1468 c         1281 g         1240 t
ORIGIN      
        1 gccaggcaga aaaaagccga ggcggaggcc gcggcggaag cagccagacg taaggctgag
       61 gaagagaaga agaaggcgga agaagagaga attcggaaag cagaagaatt aaagcgcgag
      121 cagcagcgtg aggaagagcg gaagcggaag gaagccgtcg tggacaaaga acttgaggaa
      181 ctgaagaaaa agctcgggaa gtaaaagctc cagacaatat ggaccgaaca tcttcaagca
      241 gtcattctta gttcttgaag aatgccatat tgtagaagta gttcttcagc tcccctactg
      301 ttaaggcaaa tacaccgtca ttatcacaat ctctggcaat gccgttttta ctgggctcgc
      361 cactgcccca tggattgcga acaagcacct taccgcttct ttcgtcatat ccaataacgc
      421 tgtaggcatg attattggaa agcccctcca ggggactgcc ggccttttga gtttcagctt
      481 cgtctttgtg caaacccaga gaaacaggct taccggcttt cagactggca gaaatggaag
      541 cacctatctc ttgctcagac ttaccggcaa tttcaataac tgctacctct ccagtcgcca
      601 gaagactcag cccattggca atggtatcgc caccgtcgct tccttcctgc gcgataccgg
      661 tttcacgatc gcgagcctca ggtttcacca gcccatgctt aattcgatag gcaccataag
      721 ccttctcgat aagctgaggc cagatgcccg actgactgga gcgactatag agcatacgtt
      781 ctgcttccgt tggtctggat acaacaaaag gctcgtcggg aattgccgga aagcgcacgg
      841 taaaggttcc gtcggcatta gaagtaatca tattcttgat ggtctgcggc gaagcctctg
      901 ccagggaagc cagaccggcc ataaagtagc aattgcccac gctgccctga cgaacggcag
      961 cagccctgat accaccacca ccacctatgc gctcgtcgcc aaaaacagac tcagttccct
     1021 tttcaaggct atcagagcct gaattgaccc acccctccat agtcctggca gcggcgtatg
     1081 cgggagcatc agccagggtc ttcaatcctt tctggaagga ggacaaatcg actcgtttct
     1141 ttgctagtgg attccaatca ctaacagtga atttatcaaa gtcggcaaga aactgggcga
     1201 ggcgatcacg ctctgctgca tcagaagtag acgccacagc agcacgaacc tcttcctcgg
     1261 tgacagattt accgggttcg gagagtagct tattagttac cacatcctcg tagttcttag
     1321 cactgtcact gagcgccttc tcgctccggg ctttagcgga gaagtcctgt aagtccgtaa
     1381 gagttattcc attgtcgtcc caaccgagaa agccggttcg ctgtcccttc aagtcggcgt
     1441 agcgcagttg cagcgccgca acaaactgag catcctcgcc cttgaagtcg tttgagacaa
     1501 ttcctcttgc tagttccttc ctgtcaattg aaccgttttt atcgagatca agacgagact
     1561 cgtttttcaa atacaagccc gccagcttat cgggttgaaa attgggaaat tcctttctaa
     1621 ctgactcgct actgctcata tgggttgtta ccgccgttct gtcggtgagg cgcaagcgac
     1681 caccgtccac ttctatctta gtcacatccg ctggggcgct attctggtcc agccattgtg
     1741 ccttagcatc agctcttgta taaaccctcc cgtcagagag tttcaccgag cgcaggttct
     1801 tatcaggaaa atactcgaag gaaccaacca aaccgtcctt aaaattgata gaggcgacag
     1861 agccatcgcc gtttctcaac acacttgaat ccggcttgaa gaaagagcgg ccgccgtaga
     1921 agaagcttgc cccggcagcc gcactagata ttgttttcga ctcgttggaa gcatcgctgt
     1981 ggccgctttt gctggaatta gaaccgggag aggaatttgg ctgctctccc atgaaaccaa
     2041 gcagcccgga gcctatggcg cctgcaagct tgccggtctt gccgtctggc accggacaga
     2101 caggctcacg ttcactgtct ttgcgggccg cagcctgcct ttcactcaaa gcagccagat
     2161 actgcccctg cagcggggat aagggctgag tctccaattc cgtagcctta ctggtttcac
     2221 ggatgcctga actttctgca ctcatctaac aatccccaag tcaaatcggc aaactcatat
     2281 aaacaagttc tacgcaactc aaggggtgaa taaattgagc agaaacgaaa ctgcggcccg
     2341 gggctaactt gagccgcctc cagtaaacaa actaattctc ggaacgtgac aagtccatag
     2401 cactgcaggt gggactgcag ttagtctgca aggcttgcca gactaatcaa aaaaccacag
     2461 gcgattggaa aacataaggc tctcttcact gagcccggta gacagagata cgaacagacc
     2521 agcggcacgc aagcaaacct gaaaaatcca acctcaagaa gcgaccataa gatcgcctgg
     2581 ttgttttttg taccgtactg aaatttgcaa ctcctcacca agagcagcaa caactcgctc
     2641 ggcacgttca attgaaattc cgtggtagtc atttcgctca tcgcgggaga tctgagagac
     2701 atccacagaa aggcgccggg ccagctccga ctgagagatt ccattggcta ttctcagcgc
     2761 cacaagcaat ggtccaattt ctgtaagttg tgtaaacacg ccaaagtcac ggcgcttaac
     2821 gcgctcgtac cactcaacct cttccttcag ctgcgcattg aaagaaagaa ccggctccat
     2881 ggcaaggtcg atctgctcac tagagagttt cagctcctcc aactttcgcc gctgctcctg
     2941 aattactctg ctgtctgatt cgagacgctt gagacattcc tgatactctg tttccgtctt
     3001 aatcactggc cctctcctga agaagcttta tgattccctt ttgccgatgc tcagttcgag
     3061 attttctgaa tgctgcagga aactgcagat cgttaccaaa caaatccttg atgcctgacg
     3121 actgtccaaa gtgcggataa agctcaaccc tgtagaagtg ccacattggt agctgctttt
     3181 tggttgaatt tgcataagga gtgcgactgg cgggagccca cgtccaaacc ttattaggat
     3241 caagcttgtt taaatcttgc tgcaacgcac ctgaagctaa ataggcaagc tcacattcga
     3301 aatagccatc aatgtcattg ggatggtcct tctcctcgac aaaggaaccg tcaataaaaa
     3361 tgttggtaac accgaccatc catagctgat tggtcatgac gccaagattg tcgaccaacc
     3421 gagcgcgcca gattgcatcc caatcttttg aggacccctc gctcggcacc acaagcgaac
     3481 tctgcctcaa ctccgcaagc gtgagagggt agtctcccgg tgggagtaaa ccgaattcgt
     3541 caaatggagg aagaatcata catttaacat ataacacaca cttgacaaat atgtcaaata
     3601 caatcaaaag atcgaagata ccgaaaggga cgtttagtac cgatcttctt ttccggtaca
     3661 ccggccaaat accgccattt aagcgacgcc ggattctgcc aactgtcaaa actcagtgct
     3721 tacgtagtat tgagcagata ttccgcatca tcaacctcta tggctatgct tagcttttca
     3781 ccgggtgcaa aaggtactcg caacgcactc ttgacggtaa cctttgcaat aactccaaga
     3841 tgaggacctc agtcttttgc tgtaggctgc cgaatgtcgt ctgcctttac tagaatgcaa
     3901 gaattataag gaaagaaacc tggcttgttt acttgccgct tctcatctga agcaccggcc
     3961 acggctgtcc aaggaaagca ctgcccatag aaaacaccta gtgacatcaa gagcgctatg
     4021 gattgaagtc tcagcatctc tcaactgtta acgacaaatc cttcaggcag gtatagcaac
     4081 accactacta ctcccctctg acaaaagcat tataatcgaa tggaatgcca tccttaagca
     4141 ctagggttag ctcatgcaaa cggagaacac gaagaatatg cgcaccgtag ctggggcgct
     4201 gcttttgctc tcggcagcag cgctagtatt cggacgtcat tccgaatctc agattccatt
     4261 tgattcagcc atttggaagc accaggtaaa cagcagcgag cgccttagaa tggttggaga
     4321 tctcaaaacc aaactgatga gcagcggtcg agaggaaata gagcaaatac ttggtgaacc
     4381 cagaaggggc ttcgacagcc tccatccaaa tgactactat tacctactgg gcactcagga
     4441 aaggctcctg gatactgatg gtatttggct acgcatcacc ttccaaaacg atcgtgtatg
     4501 cgacatgcaa gtgattcacg actaatgacg ccgattatca atcggctatt gtaccaatcg
     4561 gtgattgcat tgatggaaag atcgccctcg gctgatttgc aattctagac agcattctgc
     4621 accatgcaga aggtctctct aagtgcccca agaacaatca ctgcagccta cttcgaacta
     4681 cttcgcccgc accggcgtca ccagggagcg gttctgcaat tcttcattgc cttccctggc
     4741 caccaggtcg ccttccttca agccaccaaa gacctctacc tgatcgccca tgatctgccc
     4801 cttgcgcaca gagacccact ctacccgccc atcgctcacc ttacacacat aggtatcgag
     4861 aggcgtggag ataacggcgg aaaccggcac aaagagggag ttttcacgcc ttcttgtggg
     4921 ccagtagact ttgcagaaca tacctggcaa gacttcgtac ttggggttga ggaagttcaa
     4981 ttccaccggc atggttcgcg tagccttatc gaggttgttg ctgatacgag cgacggtgcc
     5041 cacaaagcga cggccgggga aggcagaaat ggtgaactgt acttccgagc caacgactac
     5101 accggcggta tcaatttccg gtaccggcgc cacgatacgc aacaggtcca attgcttgat
     5161 gcggcagatg ggcggatagg cgccggtgcc gtccggaccg acaaaactgc ccacatgcat
     5221 gcgcctttct gtgacatagc cgttgaaagg ggcgggaatt tccaggtaag aagcaaaatc
     5281 ggagaagctc tcataaccgc gaattttggc agccagttct tccttccgca tggaaagctc
     5341 gtgatctttg gcattgactc gcttcatcaa ggaattgacg tcctgccggt ccgcttccac
     5401 agactgagcc cattgca
//
LOCUS       DAOQNZ010000053         5415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6112_length_5415_cov_4.032836, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000053 DAOQNZ010000000
VERSION     DAOQNZ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6112_length_5415_cov_4.032836"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKW73_01625"
     CDS             complement(<1..240)
                     /locus_tag="PKW73_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNA72008.1"
                     /translation="MSTQFNKLGKPSGKKLDELLAELLRDDGAASPSGVIPSTNPIET
                     IVTGIAYDSRQVEPGDAFFSIEGQKQDGNAFIAQAL"
     gene            complement(224..475)
                     /gene="rpmA"
                     /locus_tag="PKW73_01630"
     CDS             complement(224..475)
                     /gene="rpmA"
                     /locus_tag="PKW73_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNA72009.1"
                     /translation="MAHKKGAGSTRNGRDSQPKRLGVKVYGGEQVCPGSIIVRQRGTK
                     IHPGKNVQRGSDDSLYSVAVGVVQFEMSRGRKLVSVYPV"
     gene            complement(562..936)
                     /gene="rplU"
                     /locus_tag="PKW73_01635"
     CDS             complement(562..936)
                     /gene="rplU"
                     /locus_tag="PKW73_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNA72010.1"
                     /translation="MFAIVEACGRQYELQPGRFIDIDMTGEEEGATHVFDRVLMIVDG
                     DKSTVGQPYVKGAKVTGKVISKLEVNEAHGRVQSNIKTAKVIVYHQKPKKGTRKKAGH
                     RVQLTRVMVDSITVDEKVVAGK"
     gene            1414..1602
                     /locus_tag="PKW73_01640"
     CDS             1414..1602
                     /locus_tag="PKW73_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72011.1"
                     /translation="MADAVNEAELGRSKYSNTPKEHLYHLLTIGWEPNSPLIIKYVAE
                     NGLQKDLNDWLAINKVKT"
     gene            1729..2268
                     /locus_tag="PKW73_01645"
     CDS             1729..2268
                     /locus_tag="PKW73_01645"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNA72012.1"
                     /translation="MLQVGKRAPDFDMPSTKDMKTLKENVKLTDYKGKWLVLFFYPLD
                     FTFVCPTELKAFSEKYDEFKKAGADVLAISTDSVYSHRAWINTSEAQGGLGSLNYVLA
                     SDITKDVARDYGVLMEDKGIALRGLFIIDPDGVLQYQVVHSLNIGRSVEETLRVLEAL
                     KTGGLCQADWKPGQKPMTV"
     gene            2534..2995
                     /locus_tag="PKW73_01650"
     CDS             2534..2995
                     /locus_tag="PKW73_01650"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA72013.1"
                     /translation="MPLRMDAPLPSLDGGTDWFNSPGVKDQDLEGQPVLIHFWALSCG
                     ICKESLPELNKWVDLYGPKGLRIISIHMPRQESDTNVPAVKECLEEWEMKQPCVVDNW
                     HTITDRFENKFVPALYLFDAEHKMRHFQAGEKAIKMTEPVVQRVLGLTQAV"
     gene            3032..4423
                     /gene="hemL"
                     /locus_tag="PKW73_01655"
     CDS             3032..4423
                     /gene="hemL"
                     /locus_tag="PKW73_01655"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNA72014.1"
                     /translation="MAVNVVADKAKGEKSGEPGKDGEGKASQGGFSLERSAKLFERAK
                     ERIPGGVNSPVRACKSVGANPIFIASADGAYMFDEDEHQYIDYVGSWGPMILGHRHPS
                     VLTAIEDAITRGTSYGAPSRLEVEMAETICQLVPSVEMVRMVNSGTEACMSAIRLARA
                     FTKRELVIKFDGCYHGHADSFLVKAGSGLATLGLSEATSSPGVPTQFTGLTLSLPYND
                     VDALRQAFAQKSNEIACVIIEPVVGNSGVIVPTAEFLKCLTGLCKESGALLIFDEVMT
                     GFRLALGGAQERFSVKPDLTCFGKVIGGGLPVGAYGGRKEIMCMVAPEGPVYQAGTLS
                     GNPLAMAAGLAQLRYLRSGKVYETLGRITDELAEGLTALLKEKNVKGQVVHVPGMLSV
                     FFTDKPVNNFDDALKCDTAFFGKVWRNLIEEGVYWPPSQFEAAFLSIMHSKADIKATI
                     EAFAKAIDKAQAA"
     gene            4452..4757
                     /locus_tag="PKW73_01660"
     CDS             4452..4757
                     /locus_tag="PKW73_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNA72015.1"
                     /translation="MQFVVTGYDGEDPKALERRLHKREEHLKGAEKMKASGTLLFATA
                     LLDDNEKMVGSILVVDFPTREELDKWLKVEPYVTGEVWLKVDVRPCRVAPMFQKVQA"
     gene            complement(4699..>5415)
                     /locus_tag="PKW73_01665"
     CDS             complement(4699..>5415)
                     /locus_tag="PKW73_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72016.1"
                     /translation="RRGEVVATAFGGNISLPHRLCPACFNKLRQPILRGYLLVQDMPV
                     PFFCAGAYEGVFQSLIHKIKYQGEAALCFDLAIVLDKLTGEFLKPEINYLLLPIPLHP
                     KRRRERGYNQCELLAKALCRRKGKAALTYKPLLLRAKDTPPQHSLTRQERIANVKSVF
                     ALKKGAANTIKDRHVILLDDVLTSGATAHEAALLLLSAGAREVVVLTLARTIKKWDGL
                     KTRPAPFETSALLGKGAHPP"
BASE COUNT         1232 a         1380 c         1465 g         1338 t
ORIGIN      
        1 cagggcctgg gcgataaagg cgttgccgtc ttgcttttga ccttcgatag agaagaaggc
       61 gtcaccgggt tccacctgcc tggaatcgta ggcgatgccc gtgactatgg tttcaatcgg
      121 atttgttgag ggaattacac cagacggcga agccgctccg tcatcacgaa gcagctctgc
      181 caatagttca tctagttttt tcccagaggg cttgccaagc ttattaaact gggtagacac
      241 tgaccaactt gcgtcctctg gacatttcaa actgcactac gccgacggcg acggaataaa
      301 gggagtcgtc tgaaccgcgc tgtacgtttt taccagggtg aatcttggtg ccacgctgtc
      361 tgacgatgat gctgccgggg caaacttgct cgccgccgta tactttcacg cccagacgct
      421 tgggttggct gtctcttccg tttcttgtcg agccggcgcc cttcttatgt gccacgttct
      481 cttctcctca agtaatttcc gaagttcaag ctttaagcct gccggaaatt ccatcctaat
      541 ctatattgat gcttttagct cttacttacc ggctacgact ttctcgtcta cagtaatgct
      601 gtctaccatg actctggtca actgcactct gtgtccggct ttcttgcggg tgcctttttt
      661 aggcttctgg tgataaacaa tcactttggc ggtcttgatg ttggactgca cacggccatg
      721 agcttcgttg acttccaatt tggagatgac tttgcccgtg actttggcgc ccttcacgta
      781 aggctgacct acggttgatt tgtcgccgtc cacaatcatg agaacgcgat caaatacatg
      841 ggtagcgcct tcttcttcgc cggtcatgtc gatgtcgatg aaacgaccag gctgtaattc
      901 atattgcctt ccgcaggctt ccactatcgc gaacattctt ctctccaacg tctcttaaca
      961 ggataggcag tcgctcttgc cctgaaataa acacatgtct aagatttcgc tccggcagcc
     1021 tgagaacttg attcagcctg aaaattggga agaatcttcg gatgaatcag agcagcccgg
     1081 cctatagagc ggacaagcga tgttgaatac taccaaaagc tatgcaatgg gtcaaacgaa
     1141 ccggccgccg ctccttcaat ataggcaaca tttgaaggat tcggagggga gcggccgccg
     1201 ctccattaca actataggca acatttgaag attcggaggg gagcggccgc tgctccatta
     1261 caacagtagg caacaattgc cggatggatt ggggccgccc ggctactact ctcaagtaga
     1321 attagaggag agagtggtgg aaccgctccg acgccgtcga tctgagggcg tgaaggtgaa
     1381 ttctggttca gcttggaact tggaggtaaa gccatggcgg atgctgtaaa cgaggcggag
     1441 ctggggcgca gcaagtacag caatacccca aaagagcatc tctaccactt gcttacgatt
     1501 ggctgggagc ctaatagccc tttaatcatt aagtatgtgg ccgaaaacgg tttacaaaaa
     1561 gatttgaacg attggctggc tatcaataaa gtcaagacct agcttggctg gctcaaacgg
     1621 tagtaacgag taattgtgag ctttccatta aacatatcaa gtattcatcg gcgccgccct
     1681 actatttgag tctcgtccct acacatagct tttggggtat ttgccgatat gttgcaagtt
     1741 ggaaaaagag ctcccgattt tgacatgccc tccaccaagg acatgaagac cttgaaagag
     1801 aatgtcaagc tcaccgatta caaaggtaag tggctcgttc ttttcttcta tcctctggat
     1861 ttcaccttcg tttgcccgac cgaactgaag gccttctctg agaaatatga tgagttcaaa
     1921 aaagccggtg ctgatgtact ggccatctca accgatagcg tttacagcca cagagcctgg
     1981 atcaacacca gcgaagctca gggtggattg ggttccctca actacgttct cgcttccgac
     2041 atcaccaaag atgttgctcg cgactacggc gtcttgatgg aagacaaagg tatcgccctg
     2101 cgcggtcttt tcatcatcga tcccgatggt gttctccagt accaggttgt tcacagcctc
     2161 aacatcggcc gttccgtgga agaaaccctg cgcgttcttg aagccctcaa gaccggcggt
     2221 ctctgccagg ctgattggaa accgggccaa aagcccatga ctgtctaatt tgctcacttt
     2281 agttcaacta aaccaggcaa cataagtcat tagagctgct tctccactta aggagaagca
     2341 gctctttcct ttaccgtctc ttctccgccg cttactgccg gccctgtccg ttaactcaat
     2401 cggcgaactt gcgcaagttt agtctttaag tatcttgtac cgttagtgga atccgcgccc
     2461 ggaaaggcta aaattaccct gtgcgtagtt gacgcggttt cacaacactt tcagcaggag
     2521 ggcgccacag cctatgcctc ttagaatgga tgctccttta ccgtctctgg atggcggcac
     2581 cgactggttc aacagcccgg gcgtcaaaga ccaggacctg gaaggtcagc cggtactgat
     2641 tcacttctgg gccctcagct gcggtatttg caaagagtct ctgcctgagc tgaataaatg
     2701 ggtagacctg tacggtccca agggcttgag aatcatctcc atccacatgc cccggcagga
     2761 gtccgatacc aatgttccgg cggtgaaaga atgcctggaa gaatgggaaa tgaaacagcc
     2821 ctgtgttgtc gataactggc acaccattac cgatcgattc gaaaacaaat ttgtgcctgc
     2881 tctttatctc tttgatgcgg agcacaaaat gcgccatttt caggccggcg agaaggctat
     2941 caaaatgacg gagcccgtgg tgcaaagagt gcttgggttg acccaggcgg tataagctaa
     3001 aaattgcgca aaccataaag tgaggttata gatggctgtc aacgtcgtag cggataaagc
     3061 taagggcgag aagtccgggg aacctggtaa ggacggtgaa ggcaaagcca gtcagggcgg
     3121 tttctctctg gagcgctccg ccaagctttt tgaacgggcc aaggagcgca ttccaggcgg
     3181 cgttaattcg cccgtgagag cctgcaagtc ggtgggcgcc aatcctatct ttattgccag
     3241 cgccgacggc gcctacatgt tcgacgaaga cgagcaccaa tatatcgatt atgtcgggtc
     3301 ctggggaccg atgattttgg gtcacagaca tccttccgtg ctcaccgcca ttgaggacgc
     3361 catcaccagg ggcaccagct atggtgcgcc gagccggctg gaagtggaga tggcagagac
     3421 tatttgccag ttggttcctt ccgtggaaat ggtgcgcatg gtcaattccg gcacggaagc
     3481 ctgtatgtcc gccatcaggt tggcccgggc ttttaccaag cgtgagctgg tgatcaagtt
     3541 cgatggctgc tatcacggtc atgccgactc ctttctggtc aaagccggtt cggggctggc
     3601 caccctcggc ttgagtgagg ccaccagttc tcccggtgtg cccacacaat tcaccgggct
     3661 caccctttcg ctgccctata acgatgttga tgcattgcgc caggccttcg cccagaaatc
     3721 caatgaaatc gcttgcgtca ttattgagcc tgtggtcggt aattccggcg tcattgtgcc
     3781 cactgccgag ttcctcaagt gcctgaccgg gctttgtaag gaatcaggcg cccttttgat
     3841 cttcgacgaa gtgatgactg gttttagatt ggctctcggc ggcgcccagg agcgcttctc
     3901 cgttaagcct gatctgacct gtttcggcaa ggttataggt ggtggcttgc ctgtgggcgc
     3961 ttatggcggc cgcaaagaga ttatgtgcat ggtggcaccg gaaggtccgg tttatcaagc
     4021 cggcacgctt tcaggtaatc ctctggccat ggcggccgga ctggcgcaat tgcgctattt
     4081 gcgcagcggt aaagtctacg agactctcgg tcgcatcacg gatgaactgg ccgaaggtct
     4141 tacggcgctt ttgaaggaga agaacgtcaa gggacaggtg gtgcatgtgc ccggcatgct
     4201 cagcgtgttc ttcacggaca agcctgtgaa caatttcgat gatgcactca agtgcgatac
     4261 ggcattcttc ggcaaggtct ggagaaattt gatcgaggaa ggcgtttact ggccgccttc
     4321 acagttcgag gctgccttcc tttccattat gcattctaaa gctgatatca aagccaccat
     4381 agaggctttt gctaaggcta tcgataaggc gcaagctgct taagtgagac caatccggcg
     4441 gagttaagga catgcaattt gtagtaactg gatatgacgg cgaagaccct aaggcgctgg
     4501 aacgtcgttt gcataagcga gaagaacatc tcaaaggcgc cgagaagatg aaagcttccg
     4561 gcacgctttt gtttgccact gcccttctcg atgacaacga gaaaatggtg ggttcgattt
     4621 tggtggtgga cttccccacc cgggaagaac tggataagtg gctcaaggta gagccctatg
     4681 tcaccggtga ggtttggctc aaggtggatg tgcgcccttg ccgagtagcg ccgatgtttc
     4741 aaaaggtgca ggcctagttt tcagcccatc ccatttcttg atggtgcggg ccagggttag
     4801 caccactact tcccgggcgc ctgccgagag cagcaacagc gctgcctcat gggcggtggc
     4861 accggaagtg agtacatcat caagcaaaat gacatggcga tctttgatgg tgtttgccgc
     4921 tccctttttg agggcaaaga cgcttttgac gttggcaatt cgctcctgtc ttgtcagaga
     4981 gtgttggggc ggggtgtctt ttgctctcag caaaagcggt ttataggtaa gagcggcctt
     5041 gcctttgcgg cggcaaaggg cctttgctaa aagttcgcac tggttgtagc ctctctctct
     5101 ccgtctttta gggtgcaagg ggatgggcaa caagagatag ttgatttcgg gcttgaggaa
     5161 ctcccctgtg agtttatcta aaacaatggc caggtcgaag cagagtgccg cctcgccctg
     5221 gtatttgatt ttatggatga ggctttgaaa aacaccttcg taggcgccgg cgcagaagaa
     5281 gggcacgggc atatcttgta ctagcaagta accccttaga atgggctgcc tgagcttgtt
     5341 gaagcaggca gggcagagcc ggtgcggtaa gctaatgttg ccaccaaaag cggtggcgac
     5401 aacctcgccc cgacg
//
LOCUS       DAOQNZ010000054         5383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6174_length_5383_cov_3.648649, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000054 DAOQNZ010000000
VERSION     DAOQNZ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6174_length_5383_cov_3.648649"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1079
                     /locus_tag="PKW73_01670"
     CDS             126..1079
                     /locus_tag="PKW73_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72017.1"
                     /translation="MNISDSHKISTLHLSKLEAGCTPLKARRRVLKEGQIIHLPVSGT
                     SRLVLERAWQHITEVFGAEPRLAHKRLKRPEFLHCVSEAKQVIFQDRELRNLLLDILC
                     NTFNSRAGIRFDPPRLRVVPPWQSLLPEPLAPEEEEEEEGACQPISRSTLAEAGLLPL
                     FSTHRDTFYANPQSQINIWLALHDVGPQDSFRFYPQYFDEPVANDSHLFNYDNWKEDV
                     GFGKSQSNHAEGKKALYPQALDEDALQDPAAFSLKEGEAIVFAAAHLHGTSPHCCPGT
                     RFSLDMRAVESEDLQPGGLQAPNRDNKSQGSSLQDYLKLEV"
     gene            1141..3330
                     /locus_tag="PKW73_01675"
     CDS             1141..3330
                     /locus_tag="PKW73_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72018.1"
                     /translation="MSNFLHKKCQDCHNTFPIETLVCPHDGNQLETTLFLSPVGHTLV
                     GRYQIDCELGRGGMGIIYRATDKQAEDPAKARVALKLLVADAAASEPLRNRFMIEAKA
                     ASSLEHPNIVKVYEYGMSEEGLPFMVMEYLDGAPLNSLIDKVPLPPELSLKFVIELCD
                     ALAHAHHRHVVHRDLKPSNIMVVEAGGEKKAVLVDFGIAKVFTQPGQVSLRLTQTGEV
                     FGSPLYMSPEQCMGQKVDHRSDIYSLGCVLYECLTGRSPFEGENFLNVIFKHVNEYPP
                     EFAGKASERAIQDVVLKALAKHPDDRYINMTAFRQHLEQCLALLSKSEAEVEKYFGQS
                     EPVQEDTPEDERFLHNLKLAEQGFAPAQLDLAYTYDDGVVVEKNLELAFDWCLKAATQ
                     GLADAQAMLGDLFKNGEGITADPEKAAYWYAKAAEQGHRGAMAALGSLYHQGLGVEPS
                     VDKAIEWYTRSAELEDESAQYLLALFYIVGDEVEKNEKLALKWFERAGQQGNAEAQYQ
                     AGLCYIQGMGTEIDAESAFYWFKLASDQGVATAQYELAMLYEQGLGTAQDTALALKWM
                     KESHFNGYEWATLVLGQWYADGINGLQQDHKRAVNLFREAAEQDVSTAMYWLGYHYQM
                     GLGVSRCNKTAAHWFKKAAHDKNSSAMLALARLLMDGLGVKQDVPEALRLLKASAELE
                     NENAELELGCYYQRQGETQEAAHWLKLALEHGATEAAEYLRRLPASD"
     gene            3623..3961
                     /locus_tag="PKW73_01680"
     CDS             3623..3961
                     /locus_tag="PKW73_01680"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNA72019.1"
                     /translation="MSQTQKQQIAKLTQFKAEFFKALSNPLRIRILDELRNDELTVSE
                     IRDRLEVELPNVSQQLAVLKAKNLVTARKQGINIYYSCSDPKIFKLLDVAREIFNSQL
                     ADIQKTLKKL"
     gene            4109..4357
                     /gene="infA"
                     /locus_tag="PKW73_01685"
     CDS             4109..4357
                     /gene="infA"
                     /locus_tag="PKW73_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNA72020.1"
                     /translation="MDQNNNKKAEGIVLDGKVLETLPSCLFTVELENGHKVMAHLSGK
                     VRKNFIKILPGDRVTIELSPYDLKRGRITYRHRNASAG"
     gene            complement(5039..5371)
                     /locus_tag="PKW73_01690"
     CDS             complement(5039..5371)
                     /locus_tag="PKW73_01690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA72021.1"
                     /translation="MLRRLAVRVLAAVTGRRGRLRPRFHLPLRVARPADVAVSPDFRR
                     RGVASDLIRHVMKMGRKLHKVRVTLEARKSNASAISLYKHLGFVKKRTIKAYYEDGED
                     GLFMEVTL"
BASE COUNT         1424 a         1466 c         1395 g         1098 t
ORIGIN      
        1 taatgttcaa ccagccgcta acaacagggt ttatgatagc ctcttccacc tgaatcttga
       61 gaaaataaaa ttcaaaagtt cactccccgc tcatcatgat ataaaagcgc aagaagcagc
      121 agctcatgaa catttcagac agccacaaga taagtacttt gcacctgagt aagctggaag
      181 cgggttgcac tcctctcaaa gcgcgccgcc gcgttctcaa ggaaggacag atcattcacc
      241 tgccggtctc cggtacaagc cgcctcgtac tggagcgcgc ctggcagcac attaccgaag
      301 tctttggcgc agagcccagg ctggcccata aaaggctgaa gagaccggag ttcttgcact
      361 gcgtcagcga agccaagcaa gtcattttcc aggatcggga gctgaggaac ctgcttctgg
      421 atatcctctg caatactttc aacagccgcg ccggtatccg ctttgatccg cccaggctga
      481 gggttgtacc accctggcag agcctcctgc cggaaccgct tgcgccggaa gaagaagaag
      541 aagaagaagg cgcctgccag ccgatctccc gcagcactct agccgaggct ggtcttttgc
      601 ccctgttcag cactcaccgg gatacgtttt atgccaatcc tcaaagtcag atcaatattt
      661 ggctggcact gcatgatgtg ggaccgcaag acagttttcg cttctatcct caatactttg
      721 atgagccggt ggcaaacgac tcccatctct tcaactacga taactggaag gaagatgtag
      781 gtttcggcaa atcgcagagc aatcatgccg agggcaaaaa ggcactctat cctcaggctc
      841 tcgatgaaga cgccctgcaa gatcccgccg cgttcagcct caaagaaggg gaggcaattg
      901 tctttgccgc cgcccatttg catggaacca gtccccattg ctgcccgggt actagattca
      961 gccttgatat gcgtgccgta gaaagcgagg atttgcaacc gggcggctta caggctccca
     1021 accgggacaa taaatcgcag ggcagtagtc tgcaagacta cctgaaactg gaggtctgaa
     1081 accggagtag tttccatcca ggcgggtata ttggccatcc cagccaaggt caggggagct
     1141 atgtcgaatt tccttcataa gaaatgccag gactgccaca acacctttcc cattgaaacc
     1201 ctggtgtgcc ctcatgacgg caaccagcta gagacaaccc tcttcctatc acctgtggga
     1261 catacgctgg tggggcgcta tcagatcgac tgcgagctgg gccggggcgg catgggtatc
     1321 atttatcgag ccaccgacaa acaggccgaa gacccggcta aagcccgcgt cgctctcaaa
     1381 ctgttggtgg cagacgctgc cgccagtgag cctttacgca atcgtttcat gatcgaagca
     1441 aaagcagcga gcagcctcga acacccgaat atagtcaagg tttacgaata cggcatgagc
     1501 gaagaagggc tgcccttcat ggttatggag tatctggacg gcgcccccct gaacagcctc
     1561 atcgataaag taccgctgcc ccccgagctt tccctcaaat ttgtcatcga gctatgtgat
     1621 gccctggccc atgctcacca tcgccatgtt gtccaccgcg accttaaacc aagcaatatt
     1681 atggttgttg aagcaggcgg cgagaagaaa gcggtgctgg tggatttcgg cattgccaaa
     1741 gtctttaccc agccgggaca ggttagcttg cgcctcaccc agacaggcga ggtcttcggc
     1801 tcaccgctct acatgagccc ggaacagtgc atggggcaaa aagtagatca ccgctcggat
     1861 atttattctc tgggctgtgt gctctatgaa tgtctgaccg gtcgctcgcc ctttgaggga
     1921 gagaactttc tcaatgtcat cttcaagcat gtgaacgaat acccgccgga attcgccggc
     1981 aaagccagtg aaagagccat tcaggacgtg gtgctgaaag ccctggctaa acacccggat
     2041 gatcgttaca tcaatatgac cgccttccgc cagcacctcg agcaatgcct ggcgcttctc
     2101 tctaaaagcg aagcggaagt ggaaaaatac ttcgggcaat cggaaccggt gcaagaagac
     2161 acgcccgagg atgagcgctt tctgcacaat ctcaaactgg cggagcaggg ttttgcaccg
     2221 gctcaactgg acctagccta tacctacgac gacggcgttg tagtagaaaa gaatctggag
     2281 ttggctttcg actggtgcct gaaagccgcc acgcagggtc tggccgacgc ccaggccatg
     2341 ttgggggatt tattcaaaaa cggcgaaggc atcacagccg acccggagaa agcagcctac
     2401 tggtatgcca aagccgccga acaggggcat cgaggcgcca tggccgccct gggcagcctt
     2461 taccatcagg gtctgggagt cgaacccagt gtcgacaaag caatcgaatg gtacacccgt
     2521 tctgccgaat tggaagacga gagtgcgcag tatctactgg cgctgttcta tatcgtcgga
     2581 gatgaagtgg aaaagaacga gaagctggcc ttaaagtggt tcgaacgggc agggcagcag
     2641 ggaaacgccg aggcacagta ccaggctggg ctttgctata ttcaaggcat gggcacggag
     2701 attgatgccg aaagcgcctt ttactggttc aaattagcca gcgaccaggg cgtggccacg
     2761 gctcaatacg aactagccat gctctatgag cagggcctgg gcacggccca agacacggcc
     2821 ctggctctca aatggatgaa ggaatcacac ttcaacggct atgaatgggc gactttagta
     2881 ctcgggcaat ggtacgccga cggcatcaac ggcttgcagc aagaccataa gcgagccgtc
     2941 aatctgttta gagaagccgc cgaacaagat gtaagcacgg ccatgtactg gctcggctat
     3001 cactatcaaa tggggctggg agtgtctcgc tgcaataaga cggcagccca ctggttcaag
     3061 aaagccgccc atgacaaaaa cagttcggcc atgctggccc tggcccgtct attgatggac
     3121 gggctgggcg ttaagcagga tgtaccggaa gctctgcgtt tactgaaagc atcggcggaa
     3181 ctggaaaatg aaaatgccga gctggagttg ggctgttact atcaaaggca aggtgaaact
     3241 caagaagcgg cgcactggct caagctggca ctggagcacg gagcgacgga agcagcggaa
     3301 tatttgcgca ggctgcccgc atcggactga ttttgcaacg gctaccctca tcgggccgat
     3361 attgcaacgg ctgcctgcaa cggcattgca aagccccccc gcatcgtact gattttgcaa
     3421 cggctgacat tgcaaaggcc accacatcgg gatgcctcag gatccggttt agttacctcc
     3481 aatctagaca gtcaatcaag tgtgtgtaaa acttgcagag ttctgcaact gattgaaaaa
     3541 ctaccagaaa gttcaagcag gatgctctca gagattaaag atactctcag agataaacgt
     3601 tgccacgccg gaagaaatca aagtgtccca gactcaaaag cagcagatcg ccaaactgac
     3661 ccagttcaaa gcagaattct tcaaggcttt aagcaatccc ctgcgcatca gaattctcga
     3721 cgaattgcgc aatgacgaat taaccgtatc agaaataaga gaccgcctgg aagtagagct
     3781 tcccaatgta tctcagcagt tagccgtact gaaagcgaaa aatctggtaa cggcaaggaa
     3841 acaaggtatc aacatctact attcgtgcag cgatccaaag atattcaagc ttctcgacgt
     3901 tgccagggaa atattcaact cccaactggc cgacattcag aaaacgctga aaaagctgta
     3961 aattcggcat atctcaacat tcattgcgat tccaaccgaa gatggggaga tcagcagatt
     4021 tcgcagagaa gatgcgctat actattgcat agttgcatac ttacgcaatt aagcaacaag
     4081 cactaaatat cagaaaagcg aagttaccat ggaccaaaat aataacaaga aagcagaggg
     4141 gatcgtactc gacggcaagg tgctggagac gttgccctca tgccttttca cagtcgaact
     4201 ggagaacggg cacaaggtca tggcacacct ctccggcaaa gtacgcaaga acttcatcaa
     4261 gatattaccc ggcgatcgag ttaccattga gcttagcccc tatgatttga agcgcggacg
     4321 gattacctac cgacaccgca acgcatcggc cggctagaga cattacctga cagccggacg
     4381 cagcagcggc tggactcagc ggcgactaga tgcaccggcg gctagaccca gcggcgacta
     4441 gatgcaccgg cgaccagatg cagccagggc tccgtcagcc agagaaagcg gcggctacaa
     4501 ttagcccggc ttacctccta tccgtggaaa aaaataggcg aaggcgaaat tattgcgttt
     4561 ttcgcttcaa ttttgcgctt tgtgcaaccg taagcgaaat tattgcgttt ttcgccttcg
     4621 cctcaatttt ccaaaacatt taccctgggt gagaattcgt ccgaagtgaa cgttggctgc
     4681 acagcgctga gcactccgca ctccgcatta agcattaagc cttccgcact ccgcattgag
     4741 cattaagcat taagccttcc gcactccgca ttgagcatta agccctccgc cctccgcact
     4801 ccgcattgag cattgagcat tgagcgttaa tccttctgca ttaagccgcc tgcattaagc
     4861 ctttccccca aatgcttatt acgctttata gtatctacgg caaaaaaagc aaaagagccc
     4921 ttcaggaagc ctgttttcac agatttacct gaaggactct cttgtagaaa aggttcgagt
     4981 ttcgcgcccc actcaaaggt tacaagcttc aagcaaggac caaacctgaa cagttaactc
     5041 aaagcgtcac ctccatgaac agtccgtctt cgccatcctc gtagtaagcc ttgatggtgc
     5101 gcttcttcac aaagccaagg tgcttgtaca agctgatggc cgaggcattg ctcttacggg
     5161 cttccagtgt gacacgcact ttatgaagct tgcgccccat tttcatgacg tgccgaatca
     5221 aatcgcttgc cacgccacgg cgccggaagt cgggactgac cgcgacatcg gcaggccggg
     5281 caacgcgaag cggaagatga aatcgtggcc gaaggcgccc gcgccgacca gtaacagcag
     5341 cgagaacgcg gacagcgagg cggcgaagca accggcggtg agc
//
LOCUS       DAOQNZ010000055         5377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6185_length_5377_cov_4.539271, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000055 DAOQNZ010000000
VERSION     DAOQNZ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6185_length_5377_cov_4.539271"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PKW73_01695"
     CDS             <1..912
                     /locus_tag="PKW73_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNA72022.1"
                     /translation="LDSLVGMGLLLRHDLERSGSLEPAAVRGGGAGRAPSVPVYKLSS
                     VAGTYLLKESPLFMGMYLKQHEELDKMWRGLKDTVSTGKPVMEVNQEERASEIFPHLA
                     ESIVPLNYAIAVDALQFVKQRLALADRSGSSLRVLDVAAGSAVWSIPFAQDNAGTRVS
                     AIDFEPVLAVTAKITERFQVREQYEDLPGNWRQVNLPEQTYDVAILGHILHSEGKVLS
                     LELLRYCARALRPGGVLVIAEFFTNKERTAPLHPLMFGLNMYLATTDGCVFSQEEMAE
                     LCMEAGYSKVVRHSAANYLSPLLFAVK"
     gene            918..2288
                     /locus_tag="PKW73_01700"
     CDS             918..2288
                     /locus_tag="PKW73_01700"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HNA72023.1"
                     /translation="MSDIAFYVKYRPEEVNDGYWSVYKRLILPGQLLSVFFVSFLLAE
                     GYAYSVTELGDYIFGAAGIDEGQLLLAVLIAGVGIPSIVTGLAALLIESRVRNSYESR
                     PNFHFPIIYILGQNGLNITFASGQGTLDWSHLNACVETAYQFCLVAGPQDVFVLPKRC
                     LTGDEEVKAVRHLIVSKVRTYLRHSGKDLPIKYEKAGIAGISIEGSGVEVGALGIITE
                     GQSADQGELKGEDNSPCDRNLGSSGQSCAGGETLAAEPDPSLPPGLKTGVAVEVIYRE
                     GEVQGAEKIYFFRKRLPMLSLQYLAFLGWLLPSTFAVGFIWGKTDLVYELFQNYGWVF
                     TFALPVYAMHAVYTYLLVLGRARLSEAMSEGRDGRSEGRERMPFVFELTEEGCGLRAA
                     ERYAVLAWWHFEEVWETKETFMLLFGRRGRAMYVIPKRAFPERVGLAYAHNLLSRKVR
                     KLLVLE"
     gene            complement(2323..2622)
                     /locus_tag="PKW73_01705"
     CDS             complement(2323..2622)
                     /locus_tag="PKW73_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72024.1"
                     /translation="MKLLVVALALAGLTFAGTEAAWSKHHGRHNGWNQGNHYGWGRGR
                     GNGCGNFNNGWNQGNGCGNFNNGWNQANGWNQNCGNRRNKNWRRLANGNFGRFWR"
     gene            complement(2862..3380)
                     /locus_tag="PKW73_01710"
     CDS             complement(2862..3380)
                     /locus_tag="PKW73_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72025.1"
                     /translation="MKTSSQLQISFANAFADLYLDKENGEFALDFRRQEVTIYLTNTQ
                     YQALVLLVQQSLEDEQFVEYLNWQKDPLQCDENEIIEVCGPDHMVCMSCAFSWERVKL
                     TFDIGMAIDLSFADFLGLASLVKEAQADLEWRRELLRLNANQESDGEGEGEADLDSDS
                     DAGPEFPRGTQD"
     gene            complement(3392..4417)
                     /gene="hemB"
                     /locus_tag="PKW73_01715"
     CDS             complement(3392..4417)
                     /gene="hemB"
                     /locus_tag="PKW73_01715"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNA72026.1"
                     /translation="MKESLTETRDKSMDYILPRVTPDVRMRRLRSKAPLRAMIRETHL
                     RLEQLIYPFFIVEGKGIKTPIKSMPGIHQQSIDEMLKEAAEVVALGIKSVLLFGIPAT
                     KDADATGAWHSQGIVQKATRELKKHFPDLIVVADTCLCEYMDHGHCGIVHEGKILNDP
                     SLEILARAAVSQAEAGADIIAPSDMMDGRIAAIRAALDANGYENIPIMAYSAKFASAL
                     YGPFREAAESAPQFGDRTSYQMDPANAREAMREIELDIEEGADIVMVKPAMPYLDLIS
                     EARYITKLPVAAYNVSGEYSMVKAAAANGWIDEKRVVMELLTGIVRAGADLIITYHAK
                     DVAEWLK"
     gene            complement(4508..5329)
                     /locus_tag="PKW73_01720"
     CDS             complement(4508..5329)
                     /locus_tag="PKW73_01720"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNA72027.1"
                     /translation="MNEPDKQGKPLAGQTILVTRARAGSEIYGQKLKALGASVLDFPV
                     LELCEPEDWQSFDKHFSDQAHFDWFVFASANAVDATVERLKQIGRFEILEKSRIAAIG
                     AATAAKLAEHGLSTCFQPSSFVAESLVTELPAAFPLADKTVFWPRTDIGREVIVDGLA
                     AQGARVYHAVVYKTVVPKEAKEKALVMASRLQAGEVQFLTMASAQSVRNFAQIMKDHN
                     LPELLARTQIAVIGPITAREAERQFGRVDIEARVHSLDGLTEAILTALKFPNHKN"
BASE COUNT         1101 a         1386 c         1516 g         1374 t
ORIGIN      
        1 ctggatagtc tcgtggggat ggggctcttg cttcgccatg atctggaaag gagcggcagt
       61 ctggagcctg cggcggtgcg aggcggcggc gcggggcgtg ctcccagtgt gcctgtttac
      121 aagctcagct ccgtggccgg cacttacctg ttgaaagaaa gcccgctctt catgggcatg
      181 tatctcaagc agcacgaaga gctggataag atgtggcgtg gtctcaaaga tacggtcagt
      241 accggcaagc cggtcatgga agtaaatcaa gaggaacggg cgtccgagat ttttccccac
      301 ctggccgagt caatcgtgcc gctcaattac gccattgccg tcgatgccct gcagttcgta
      361 aagcagcggc tggccctggc tgatcgcagc ggcagtagtt tgcgggtttt ggatgtggca
      421 gccggcagtg ccgtctggag cattcccttt gctcaggata acgccggtac cagggtgagc
      481 gccatcgatt ttgagccggt gctggccgtc accgcaaaaa taaccgaacg ctttcaggtg
      541 cgggaacaat acgaggactt gcctggcaac tggcggcagg tgaatttgcc cgagcaaact
      601 tatgatgtgg ccatactggg gcacatctta cattccgaag gcaaagtgct gagcctcgag
      661 cttctgcgct attgtgcccg ggcactgcgg ccgggtggtg tactggtgat cgccgagttc
      721 ttcaccaata aggagcgcac ggcgcctctg catccactca tgttcggctt gaatatgtat
      781 cttgccacta ctgatggttg cgtcttcagc caggaagaga tggccgagct ttgcatggaa
      841 gctggttatt cgaaggtggt caggcattcc gctgctaatt atttgtcacc cctgctcttc
      901 gctgtgaagt gagttcaatg tctgatattg ccttctatgt gaagtaccga ccggaggaag
      961 tcaatgatgg ctactggtca gtctacaagc gcctgattct gccgggacag ctgctttcgg
     1021 tcttctttgt cagctttctt ctggcggaag gctatgcata cagcgtaacc gagctgggtg
     1081 attatatctt tggtgcagcc ggtattgatg aggggcaact gctcctggcc gtgcttattg
     1141 ccggggtcgg cattccttcc atcgtgaccg gactggcggc cctcttgatt gaaagccggg
     1201 tgagaaacag ctacgagagc cgccctaatt ttcacttccc tattatctat atactggggc
     1261 aaaatggctt gaacattaca tttgccagtg gtcagggcac cctcgactgg agtcatctca
     1321 atgcctgtgt ggagaccgcc tatcagtttt gcctggtggc tggcccgcaa gacgtattcg
     1381 tgctgccgaa gcgctgcctg acaggtgatg aggaagtaaa ggcggttcgc catttgatag
     1441 tttctaaagt gcgcacctat ttgcgccata gcggcaaaga cttgccgatc aaatatgaaa
     1501 aggccggcat agccggaatt tccattgagg gaagcggcgt cgaggtaggg gctctcggta
     1561 tcatcactga ggggcagtcg gcggatcagg gcgaactgaa gggcgaggac aacagccctt
     1621 gtgaccggaa cctcgggtcc tcggggcagt cttgcgcagg cggtgaaacc ttagctgccg
     1681 agccagatcc atccctgcct cccggtctga aaacgggagt ggccgtggaa gtgatctaca
     1741 gggaaggaga ggtgcagggg gcagagaaga tctatttctt ccgcaagcgc ttgcccatgc
     1801 tttccttgca atatcttgct tttctgggct ggctcttgcc ttctacattt gccgtcggct
     1861 ttatctgggg caagaccgat ctggtctatg aactcttcca gaactatggt tgggtcttca
     1921 cttttgcgct acctgtctac gccatgcacg ccgtttatac ctatcttctg gtcctgggtc
     1981 gggctcgcct cagcgaagcc atgagcgagg gaagggacgg ccgctccgaa ggtcgggagc
     2041 ggatgccttt tgttttcgag ctaactgaag aaggctgcgg actgagagcg gcagagcgat
     2101 acgcagtact tgcctggtgg catttcgaag aagtctggga gacgaaagag acttttatgt
     2161 tgctcttcgg tagaaggggt cgggctatgt atgtcattcc taagcgggct tttccggaga
     2221 gagttggtct ggcttatgcc cacaatctac tttcgcgaaa agtgcgcaag ctgctggtgc
     2281 tggaataagc gccggccgga aaagccgctt ggggaacttg tcttagcgcc agaagcgccc
     2341 gaaattaccg tttgccaggc ggcgccagtt cttgttccgg cgattgccgc agttctgatt
     2401 ccagccattg gcctggttcc aaccgttgtt gaaattgccg cagccgttgc cctggttcca
     2461 gccgttgttg aagtttccac agccgttgcc gcgtccgcgg ccccagccgt aatgattgcc
     2521 ctgattccag ccattgtgtc tgccgtggtg tttactccaa gctgcttccg tgccggcaaa
     2581 tgttaaaccg gccaatgcta gcgctacaac gagcaatttc atgatcgcac tccttaagta
     2641 tggacattct taccgccgct gctattaagc agccgagtcg ggccgaactg aattaggtct
     2701 tctatttttg cttcctgatc gtggtgctag ctgcccctag ttctgcggca attaacttca
     2761 cttttattga gctacgagaa gatatgacgc gaacagtgag aggatgttgc cggcgtatag
     2821 cccaaaaccc aaaaaattcc caggaatttg attagaatta gttagtcttg ggtgcccctg
     2881 ggaaattccg gtccggcatc ggagtccgag tcgagatccg cttctccttc accttcgccg
     2941 tccgactcct ggttggcatt cagtctgagc agctcccgtc gccattccag atcggcctgg
     3001 gcttctttga ccaggcttgc cagaccaagg aagtcggcaa aggaaagatc gatggccatg
     3061 ccgatatcga aggtgagttt tactcgttcc cagctgaagg cgcaggacat gcagaccatg
     3121 tggtccggtc cgcagacttc gatgatttcg ttctcgtcgc attggagcgg gtctttttgc
     3181 cagttcaggt attcgacgaa ctgctcgtct tccaaacttt gctggaccag gagcaccagg
     3241 gcttgatact gggtgttggt taagtaaata gtgacttctt gccgtctgaa atcgagggcg
     3301 aattcaccat tttctttgtc gaggtagaga tctgcaaatg cgttagcgaa actgatttgt
     3361 aattgcgagc tagtcttcat tttttaccta actacttgag ccactcggcg acatctttgg
     3421 cgtgataggt gatgatcaaa tcagcaccag ccctgacaat gccggtcaag agttccatta
     3481 ccactcgctt ctcgtcaatc cagccattgg cggcagcggc tttgaccatg gaatactctc
     3541 cagatacatt ataggctgca acgggcaatt tggttatata ccgggcctct gaaattaggt
     3601 ccaggtaagg catagccggt ttaaccatga caatgtcggc gccttcttcg atatccagct
     3661 cgatttcgcg catggcttcc cgggcgttgg ccgggtccat ctgatagctg gtccggtctc
     3721 caaactgggg agccgattcg gctgcctccc ggaaaggacc atagagagcg gaggcaaact
     3781 tggcgctgta agccatgatc ggaatgtttt catagccgtt tgcatcgaga gcggctctga
     3841 tagcggcaat tcgcccgtcc atcatgtcgg aaggggcgat tatatcggcg ccggcttcgg
     3901 cttgagagac ggcggccctg gccagaattt ccaggctggg atcattgagg attttgccct
     3961 catggactat gccgcaatgg ccgtggtcca tgtattcgca caaacaggta tcggccacta
     4021 cgattaagtc ggggaaatgc ttcttcagtt cgcgggtggc tttctgcact atgccctgcg
     4081 aatgccaggc gccggtggca tcagcatctt tagtggccgg aattccgaaa agcaataccg
     4141 actttatgcc gagagccacc acttctgccg cttctttcag catttcgtcg atgctctgct
     4201 ggtgtatgcc gggcatagac ttgatcgggg ttttgatgcc cttgccttct acgatgaaga
     4261 agggatagat gagctgctcc aggcgcagat gcgtttctct tatcatcgct ctgagcggcg
     4321 cttttgagcg caggcgacgc attctcacgt cgggagtgac acggggcagt atgtaatcca
     4381 tagacttgtc acgtgtctca gtgagcgatt ctttcattta aggttcctac ctgatctgaa
     4441 gggagcagac taatagatat aaacaataaa tatatcctca aactgaagct gctatgccaa
     4501 aagcagccta atttttatgg tttgggaatt ttaatgctgt caagattgct tctgtgagac
     4561 cgtccaggct gtggacccgc gcttcaatgt ccactcttcc gaattgtctt tcagcttctc
     4621 tggctgtgat tggtccgatc accgcaattt gagttctggc caaaagctcc ggcaggttat
     4681 ggtctttcat gatctgggcg aagttgcgca ctgattgcgc gctggccatg gtcaggaact
     4741 gcacttctcc agcttgtagt cggctggcca tgaccagggc tttttccttt gcttctttcg
     4801 gtacgacggt tttgtagacc acagcgtgat acacccttgc tccctgagcg gcgaggccgt
     4861 ccacgatcac ctctctgccg atatcagtgc gcggccagaa tacggttttg tccgccagtg
     4921 gaaaggcggc gggcagctcc gtcaccaggc tctcggccac aaaactggag ggttggaagc
     4981 aggtagagag tccatgctct gctaatttag ctgccgttgc cgcaccgata gcggcgatcc
     5041 ggctcttctc cagaatttca aatctgccga tttgtttgag tctctcgacc gtggcatcta
     5101 cggcgttggc actggcaaat acgaaccaat caaagtgcgc ctggtcagag aagtgcttgt
     5161 cgaaactttg ccaatcctcc ggctcgcaca actccagcac gggaaaatcc agaactgagg
     5221 ctcccagggc ctttagtttc tgaccgtaaa tttcgctgcc ggcacgtgct ctggtaacta
     5281 gaatggtttg accggccagt ggcttccctt gcttgtcagg ctcgttcatc gaattcgctc
     5341 gttagtttga tttgttttga ccaatcaggg cggtgat
//
LOCUS       DAOQNZ010000056         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6246_length_5345_cov_3.954442, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000056 DAOQNZ010000000
VERSION     DAOQNZ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_5345_cov_3.954442"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PKW73_01725"
     CDS             complement(<1..739)
                     /locus_tag="PKW73_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72028.1"
                     /translation="MTSLETKERSLGRGELKVLADLSKLLGIDKYFQGEVTLQGHDPI
                     LKSPHHLSEATAYALLAEAMAAVSIANLGGAKANSLSINVTDAIHFLHSSHFLWQSGY
                     GMTVGAEYIPTNGIFACRDGKHIMIEAGPPYTKLERGYLNFFDCGNNRESIAREIAKY
                     DGEELQEALSQKGLPACLAYTKEEWRAHPQGQELSKVPVIEIEKLADGKPVPFKKNDG
                     LGPLHDIKVLDFTHVLAGPRSARTLANY"
     gene            complement(764..1606)
                     /locus_tag="PKW73_01730"
     CDS             complement(764..1606)
                     /locus_tag="PKW73_01730"
                     /inference="COORDINATES: protein motif:HMM:NF018936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 family protein"
                     /protein_id="HNA72029.1"
                     /translation="MGEPQAANDESLDRHWSEEVEKVGLTHKRFTELYALAIKSLKPD
                     AEVKVLDEDRIEIRFAEGVAGISYTENPWRLCKDLPGERRANLRICLADAVRNEIMDD
                     QVVQETDIVPLIKGDDFLKAAKHSGVKVYHEEVSKGLHLVYALDEEDALTFIDQNRLD
                     ALKLSKTDLKELALANLHKRVADNTFLNTANDRYGMPTIDGVFESSLVLFETFMTAMS
                     EQFQNDLMFVIPNRDIVLLGPRSDQELQAWFKSHAKKSFKEYPHPISTRLYTWKEGKI
                     IAID"
     gene            complement(1713..2813)
                     /gene="ald"
                     /locus_tag="PKW73_01735"
     CDS             complement(1713..2813)
                     /gene="ald"
                     /locus_tag="PKW73_01735"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNA72030.1"
                     /translation="MAKVGIPKEIKDNEYRVAITLAGVKSLVSDGHTVYIEKNAGIGS
                     GMTDDEYKAAGAKILPDAASVFGESDLILKVKEPQAQEVPMLRKGQLLFTYLHLAAMP
                     KLAHDLAKTGATCVAYETVQLANGQLPLLTPMSEIAGRLATQIGANYLERPMGGRGVL
                     LGGVPGVEPGEVIIVGGGVVGTNAAKIAVGLGARVTIFDLSLERLRYLDDVFGGQVRT
                     VFSVGHYLESLLPHADLVIGAVLIPGKQAPKLVTREMVSKMKKGAVIVDVAIDQGGCI
                     ETIKATTHSQPVYEVDGVVHYGVANIPGAVPWTSTRALTNATMPYAMKLAKFGYQACL
                     DDPALNLGVNIHEGQIVHHGVLEAVGTPVAAK"
     gene            3075..4247
                     /locus_tag="PKW73_01740"
     CDS             3075..4247
                     /locus_tag="PKW73_01740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNA72031.1"
                     /translation="MSEIVIVNGARTAFGAFGGGLATKSATDLAVEAGRGAIERSGVD
                     KELIDSVIMGNVIQTSKDAIYLARHVALRNGLKESTPGHILNLLCGSGVSAVATAAMQ
                     IQAGMASVVLAGGTDALSMTPYSNWSNRWGGRMGHMELWDGLDIRDTYAKASMGETAE
                     NIQEKYKISREDQDEFALRSQTLAAQAMQSGRLREEIVPVEVESKKGKVVIEKDEHAR
                     AETTLEGLAKLKPVFRKGGSVTAGNACGIVDGAAALVVTSAATAEKLSLKPLVKVVSW
                     AVTGVPPEIMGIGPVPAIPMALEKAGLKMDQIDLFEINEAFAVQYLACEKELNLPREK
                     VNVNGGAIALGHPFGATGARLLLSISMELVKRKARYGCVSLCIGGGMGIAMILERI"
     gene            4257..>5345
                     /locus_tag="PKW73_01745"
     CDS             4257..>5345
                     /locus_tag="PKW73_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72032.1"
                     /translation="MRETAEERERKRVLAMLQGTLFPCVRARVTFSRKNADILESRPL
                     GRSNASKGYNGKLVYLPLAEQLAVGLLYDQGESYVHVRDVELGTWNLSIKAAMDIATA
                     NLQAISPTDFRQIEPGIKLFVATWPDVHTSARLLMPEIFTRVPDVLTGPVVAMAPSAD
                     RILVADGDDISALMLMMEISASLWQEARPIPMQPLVLKNGAWDYFQLPPDHPCYFELK
                     LFNLAAINLAYEQDSERIAIETKDSLFAPTLEVVQDMGTGSIYTKAIVPQGRRSSVPR
                     ADYLEFFREGQEGQYESLGVCSLARAFALLKNRIEEDYGSYPPRVLVRSFPDQRQIMN
                     LGFEKESTLPVYVPPPEGIEPAVMEADDE"
BASE COUNT         1113 a         1341 c         1552 g         1339 t
ORIGIN      
        1 cgtagttggc cagggtgcgg gcgctgcgcg gaccggccag cacgtgggtg aagtcgagaa
       61 ccttaatgtc atgcagcggt cccagtccat cgtttttctt gaagggaacc ggcttgccgt
      121 ctgccaactt ttctatttca atgaccggca cttttgatag ttcctgaccc tgcgggtgcg
      181 cccgccactc ctcctttgta taggcaaggc aggccggcag ccccttttga gagagggctt
      241 cttgcaattc ctcgccgtca tatttggcga tttctctggc gatggattcg cgattattgc
      301 cgcaatcaaa gaagttgaga tagccgcgtt cgagctttgt gtagggtggt ccggcttcga
      361 tcattatatg cttgccgtct cgacaggcga aaatgccgtt ggtgggaatg tactccgctc
      421 ccactgtcat gccgtagccc gattgccaga gaaagtgcga ggagtgaaga aagtgaatgg
      481 catcagtgac atttatactg agggaattgg ccttcgcgcc tcccaggttg gcaatcgata
      541 ctgccgccat ggcttcagct agaagggcat aggcggtggc ttccgagaga tggtgcggac
      601 ttttgaggat ggggtcgtga ccctgcaagg tgacttcacc ctggaagtat ttgtctatac
      661 caagcagttt ggacaggtcg gcgagaacct ttagttctcc tcgtcccagg ctgcgctctt
      721 tcgtttcgag gctggtcacg tgtggctcct tttgagttta tggtcagtca atggcaatga
      781 ttttgccttc tttccatgtg tatagtctgg tgcttatggg atgcggatac tccttgaaag
      841 acttcttggc gtgcgactta aaccaagcct ggagttcctg gtcggacctc ggtccaagca
      901 atacaatatc tcggtttggt atgacgaaca tcaggtcgtt ttgaaactgt tcgctcatag
      961 ctgtcatgaa agtttcaaaa agcaccaggc tggattcgaa gacgccatca attgtaggca
     1021 ttccgtatcg gtcatttgca gtgtttagga aggtgttgtc ggccactctt ttgtgaagat
     1081 tggccagggc tagttccttc aaatccgttt ttgagagctt cagagcatcg agtctattct
     1141 ggtcgataaa agtgagggca tcctcttcat cgagggcata gaccaggtgc agtcccttgg
     1201 agacttcttc atggtaaacc tttacacctg agtgttttgc agctttgaga aagtcgtctc
     1261 ctttaatgag cggcacaata tctgtttctt gtaccacctg gtcatccatt atttcgtttc
     1321 gaacggcgtc tgcaaggcag atccgaagat ttgctcgcct ttctccgggc aagtctttgc
     1381 aaaggcgcca tgggttttca gtgtaggaaa taccagcgac tccttcggca aaccttatct
     1441 ctatccggtc ttcgtctaat actttcactt ccgcatccgg cttaaggctt ttgattgcca
     1501 gcgcatacag ttcggtgaag cgcttatgag tcaacccgac cttttctact tcttccgacc
     1561 agtggcgatc cagggattca tcattagcag cctgaggctc tcccacgagt tctccttccc
     1621 gaccggtttt gtcggcaaaa ataaaacctc cgcctgtact tcccgaggaa agttgagcgg
     1681 aggcttattt tagctttttg tctgcgccgg tattacttgg cagcgacagg agtaccgacg
     1741 gcttccagta caccgtggtg tacgatctgg ccttcgtgga tgttgacgcc caggttgagg
     1801 gcgggatcat ccaggcaagc ctggtagccg aacttagcca gcttcatggc ataaggcatg
     1861 gtggcattgg tgagggcgcg ggtggaagtc cacggtacgg caccggggat attggcgacg
     1921 ccgtagtgca caacgccgtc gacttcgtag accggctgtg agtgggtggt ggctttgata
     1981 gtttcgatgc aaccgccctg gtcgatggct acgtcgacga tgacggcgcc tttcttcatc
     2041 ttgctgacca tctcgcgggt aaccagcttg ggagcttgtt tgccggggat gagtacggcg
     2101 ccgatgacga ggtcggcgtg gggcaggagg ctttccaggt agtgtccgac cgagaatacg
     2161 gtgcgaacct gaccgccgaa gacgtcgtca aggtaacgca gtctttccag cgacagatcg
     2221 aagattgtga cgcgggcgcc cagaccaaca gcgatcttgg cagcgttggt accgacgaca
     2281 ccgccgccga cgatgattac ttcaccgggt tcaacgccgg gcacgccgcc caggagaaca
     2341 ccgcgaccac ccatgggacg ctccaggtaa ttggcgccga tctgagtagc caggcgtccg
     2401 gcgatttcag acatgggggt gaggaggggg agttggccgt tggccagttg cactgtttcg
     2461 taggctacgc aggtagcgcc ggtcttggcc aggtcgtggg ccagcttggg catggctgcc
     2521 aggtgcaggt aggtgaagag caactgtcct tttctgagca tgggcacttc ttgcgcctgg
     2581 ggctctttga ctttcaggat caggtcggac tcgccgaaga cggaagcggc atcaggaaga
     2641 atttttgctc ctgcggcttt gtattcgtcg tcggtcatgc cgctgccgat accggcattc
     2701 ttttcgatgt agacggtgtg cccgtctgaa acaagcgatt tgactccagc cagagtgatg
     2761 gcaacgcggt attcgttgtc tttgatctct ttcggtattc caaccttagc caaggttcga
     2821 ccctccaatt agttatgaaa gccattgcgg cacttcggaa attttagcct gacaagatgt
     2881 cagaactata tgctcttgat agatgtttta tgattgccta agctttggga tgtaaagtgc
     2941 tcgtatcgcc cgccttttct cgggcaggcg ttgcactgtc tgataacggc ctggggcgac
     3001 caggccttca agggtgcgat actaaggggg catttgcagt atcttccgat cgtcttttag
     3061 aaaggttgtt tcccatgagc gagatagtga ttgtcaatgg tgccagaacg gcatttggag
     3121 cctttggtgg cgggcttgcc acgaaaagtg ctaccgatct tgccgtagag gccgggcggg
     3181 gagctatcga gcgctccggt gttgataagg aactgatcga ctcggtaata atggggaatg
     3241 tcattcagac cagcaaagat gccatttatc tggcgcgcca cgtagccttg cgcaatggtt
     3301 tgaaagagag tactcccggt cacatcctca atctgctttg cggctccggg gtgagcgccg
     3361 tggctaccgc tgccatgcag atccaggcag gcatggcttc cgtggttctg gccgggggca
     3421 ccgatgctct ttctatgacc ccctacagca actggagcaa ccgctggggt gggcgcatgg
     3481 gtcacatgga actgtgggac ggtctcgata ttcgcgacac ctatgccaaa gcttccatgg
     3541 gtgagaccgc cgagaatatt caagagaagt ataaaatcag ccgcgaagac caagatgaat
     3601 tcgccctgcg cagtcagact ctggctgccc aggccatgca gagcggccgt ttgcgagaag
     3661 agattgtgcc ggttgaggtg gaatcaaaga aaggcaaggt cgtcattgag aaggatgagc
     3721 atgcccgggc tgagacgact cttgaggggc tggcaaagtt gaaaccggta ttccgcaaag
     3781 gcggctcagt aacagccggc aatgcttgcg gtatcgtaga tggtgcggcg gccctcgtgg
     3841 ttacatctgc cgccacagcc gaaaaacttt ctttgaagcc gctggtgaaa gtagtatcgt
     3901 gggcggtgac gggcgtgccg ccggagatta tgggtatcgg tccggtgcct gccattccga
     3961 tggcccttga gaaagctggt ttgaagatgg accagatcga tctctttgaa attaacgagg
     4021 cttttgccgt gcaatatctt gcctgcgaaa aagagttgaa cttgccccgt gaaaaggtca
     4081 acgtcaatgg cggcgccatt gccctcggtc atccctttgg tgccaccggt gccagattgc
     4141 ttctttccat ttcaatggaa ctggtcaagc gtaaagctcg ctatggttgc gtcagccttt
     4201 gtattggcgg cggcatgggt atcgccatga tattggaaag gatctagcga aggtcgttga
     4261 gagagacagc agaagaaaga gaaagaaagc gagtgctcgc catgttgcag ggcactctct
     4321 tcccctgcgt gcgcgcccgg gtgaccttca gccgcaaaaa tgccgatatt ctggagtcga
     4381 gaccacttgg tcgatccaat gcttccaaag gttataacgg caaactggtc tacttgcctc
     4441 tggcagagca gcttgccgtt ggtctacttt atgatcaagg cgaaagctat gtgcatgtgc
     4501 gtgatgttga gcttggcacc tggaatttgt ccatcaaggc tgctatggac attgccaccg
     4561 ccaacctgca agccataagc cccacggatt ttcgacagat tgagcctggc ataaagctct
     4621 ttgtagcgac ctggcctgac gtgcatactt cggcgcggct tttgatgccg gagatattca
     4681 ccagagtgcc cgacgttttg accggaccgg tggtggccat ggctccgtcg gcagaccgca
     4741 tactcgtggc tgatggagac gatatctcgg ccctcatgtt gatgatggaa atttccgcgt
     4801 cgctctggca ggaggctcgt ccgattccca tgcagccact ggttttgaag aatggtgcct
     4861 gggattattt tcaattgcca ccggaccatc cctgctactt tgagttgaag ctcttcaatc
     4921 tagccgccat caacctcgct tatgagcaag actccgagcg cattgctatc gagaccaagg
     4981 atagtctctt cgctccgacc ctggaagttg tgcaggacat gggcacgggc tcaatctata
     5041 cgaaggctat tgtgccgcaa ggcaggcgca gcagtgtgcc ccgcgctgac tatctggaat
     5101 tctttcggga aggtcaggaa gggcaatatg aaagtcttgg cgtttgcagc ctggctcgtg
     5161 cctttgcgct attgaaaaat cgcatcgagg aagattacgg ctcctatcct ccccgggttc
     5221 tggtgcggtc ttttcccgac cagagacaaa taatgaattt gggttttgag aaagagtcga
     5281 ccctgcccgt ttatgtgccg ccgcccgaag gaattgagcc tgccgtgatg gaggccgatg
     5341 atgag
//
LOCUS       DAOQNZ010000057         5325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6278_length_5325_cov_3.775901, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000057 DAOQNZ010000000
VERSION     DAOQNZ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6278_length_5325_cov_3.775901"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKW73_01750"
     CDS             complement(<1..420)
                     /locus_tag="PKW73_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNA72033.1"
                     /translation="MLFASPEWLWAALLLPVFWFVLRRRGKIGSTHVGSLGKRPGSRI
                     LIYLPGVMLSLGFLALVVAMARPQEVFYDSDVTVKARDIIIAVDKSGSMSSPISGPLP
                     RSVVGVTDLDREWPGKPPKPNTPVETFRGQESHSRLEV"
     gene            967..1935
                     /locus_tag="PKW73_01755"
     CDS             967..1935
                     /locus_tag="PKW73_01755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNA72034.1"
                     /translation="MPLHDSFKTTAGANVRLTKLASIPSIQDLDFAMAQASKHPRAII
                     ELPWKAASSPTTFVLRVSTADGSEEGPSWTLHAGESLDAAVLWSHDSMDTFLIQSLIS
                     AEGDPNRALQTYVDTSKQAPALEEPTPPPRPGAPVRSARPDLPAPVELDRGLLEALQW
                     QMRRQETGLFSEAYAYHLLIQEFNRYQRYQTPFAFVLFELALKLNDGTLSLPPVRLLH
                     ESANRIQGVGRVLDCLCHYKDPKLCLVLPHTGCNEAMQLCARIEEALLSSPLAPGLDR
                     SNICICFGVASVPDTCEHPGILIAAAQEALEQAKRTGSTVVLFPSS"
     gene            complement(1932..3848)
                     /locus_tag="PKW73_01760"
     CDS             complement(1932..3848)
                     /locus_tag="PKW73_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA72035.1"
                     /translation="MERESWNPGLPLFPAGSGVFDLEFTTVGFVENALSHFLLSWSGC
                     LEVAAINPVLRLMRLGWHFAGQRRPLIVLYLVLFLLAQIVALAEPYVIGRLLNCLQAA
                     TAPGALPKLKEQVLVYLAIFAATQLGFWLFHGPGRLIERYAAFHIRADYKMHLFEVLL
                     AHPLQWHRDHHSGDNIDKVNKASTALWRFFDESFDLLYMIFRLCGAVLVLLFFLPWVG
                     VAALVATSLAAAIIVYFDRRLARVYMEQNLIENRAAAALFDYLSNVVTVLTLRLEKRL
                     SLSLREKLMAALPVFQRGSWLNEWKWCLTTILITLMIVCCMAGYVWQALVGGAVLMAG
                     TFFTLFEYLRRIGDSFYSFALTYGTVVQNAADVESANAILLEARSQEALPLPVLGPAL
                     PENWRRIVVENLSFNYEDDEHREHHLYDVSIVLERGKSVALVGPSGSGKTTLLSLLRG
                     IYFTEAAVSCDGAPLPYGLKHLAACTTLMPQDPEIFNDSVEDNISFATAGSEVDLKRA
                     LVLARFEPVVERLNQGLKTNVQEKGVNLSGGEKQRLALARGLFFAQDSDIVLADEPTS
                     SVDAENERLIYQGIIEECRDKCFVSAVHKLNLLDLFDYVYLFGEGRIVEQGSLSELLI
                     AGGEFSRVYRENYG"
     gene            complement(4009..4806)
                     /locus_tag="PKW73_01765"
     CDS             complement(4009..4806)
                     /locus_tag="PKW73_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72036.1"
                     /translation="MSTNNQINLCIPSTGLGAESDWRRLYEAAFPVDERMPVSEISSL
                     LSTTGMLLHRTTDKDGALLCFSLVTPMSNFSLLAYIATDQTRRSGGVGTKHMQALLVS
                     LREKFPQHLGLFLEIESTKEKGLSPEEEKNRQRRLQFYLKLGAKRLIDRDYLLPSYVP
                     GTKARYGELLWFEYERKLEENQHKDIGPVICEIYRRGYGVSHLDEQFTRVMSQFPSVD
                     GATCLDRDAVKQAEAESEKGTGFWKWLKAFLKRIFGGKWRSSGKSPK"
BASE COUNT         1379 a         1529 c         1359 g         1058 t
ORIGIN      
        1 cacttccagc ctggagtgcg actcctgacc acgaaatgtc tccaccggtg tgttgggttt
       61 gggcggcttg cccggccact cgcgatcgag gtcggtaact cctacaacgg agcggggcag
      121 cggaccggag atgggactgg acatggagcc ggacttgtcc acggcgatga tgatatcccg
      181 tgccttgaca gtcacgtcgc tgtcgtagaa aacctcctgc gggcgagcca ttgccaccac
      241 gagagcgaga aagcccagcg acagcatgac ccccggcagg taaatgagaa tgcggctgcc
      301 tggtcgcttg ccgagggagc cgacatgggt cgacccgatc ttgccccgac ggcgcagtac
      361 aaaccagaac acaggcaata gcagagcggc ccagagccac tccggagatg caaataacat
      421 gtgaaactcc tgatagttaa acccgccttc aaggcaggaa ttagaaactc cagcaaaatc
      481 cagggccagc agagagcgtt agttccctga ctgttaccag accggcctga cacttcgaac
      541 attcgattcg aagaaaatgg gggcgcaggc tcgggggaga tgctttagag atctaagtta
      601 agaaactcgg tttggaagaa aaaatacaga agcagcctaa gtaactcagt catgagatct
      661 caatcagatc tctttacagg ctactaaatt agatatcaca gaggcccggc tgagccatcg
      721 aggcgcgttg ccaagcgaag tccatcaata cgctagctag gccacgctga aatcaagccc
      781 gagagcaccg ccctcctgac atcccgggta ttcctggcgc ctacgccctg cgtctgccga
      841 caggttcagc caggtcgccg acgtaaaggt taaagctcaa gatccagccg attttttggt
      901 gtatttgagc ggcgctctgg gtaataaacc atcagtacta ccctgactca gccaatttac
      961 cagtctatgc cactacatga ttcgttcaag acaaccgctg gcgccaacgt ccgacttacc
     1021 aaattagcat ccattcccag cattcaagac ctggattttg ccatggccca ggccagtaaa
     1081 catccaagag caatcatcga attgccatgg aaagcggcct catccccaac cacatttgtg
     1141 ttgcgagtga gcacggccga cggttccgaa gaaggtccca gttggacctt gcatgccggg
     1201 gagtccctgg atgcagccgt gctgtggagc cacgacagca tggacacttt tttgattcag
     1261 agtttgattt ccgccgaagg cgatccaaat cgcgcactcc agacctatgt ggacacatca
     1321 aagcaggcac cggctctgga ggaaccaacg ccgccgcctc gaccgggagc accggtgcgc
     1381 tcggccagac cggatcttcc cgcaccagtc gagttggatc gcggtcttct ggaagccctg
     1441 caatggcaga tgcgacggca ggaaaccggg ctgttctcgg aagcctatgc ctaccatctg
     1501 ctcattcaag aattcaaccg ctaccagcgt taccagaccc ctttcgcctt tgtgcttttt
     1561 gaactggccc tcaagctcaa tgatggaacc ctcagcttac cgccagtgcg ccttttgcac
     1621 gaaagcgcca atcgtattca gggcgtcgga cgagtgctcg actgcctctg tcattacaaa
     1681 gaccccaaac tatgtctggt tctgcctcac accggctgca acgaagccat gcaactttgc
     1741 gcccgcatcg aggaggcact actctcaagc ccactggcac cgggtctgga ccgcagcaat
     1801 atctgcatat gctttggcgt ggccagtgtt cccgatacct gcgaacaccc gggcatcttg
     1861 atcgctgccg cccaggaagc cctggaacaa gccaaacgca caggcagcac cgtggtgctc
     1921 ttccccagca gctagccgta gttttctcga taaacccggg agaactcacc accggcaata
     1981 agcagctcgg acaggcttcc ctgctcaact atgcgtccct ctccgaagag ataaacatag
     2041 tcgaaaagat ccagtaaatt gagcttatgc acggctgaca caaaacactt atcccggcat
     2101 tcttcgatga tgccttgata aatcaagcgc tcgttttcgg catccactga actggttggt
     2161 tcatccgcca gaacaatgtc gctatcctgg gcgaagaata gccccctggc cagagccaat
     2221 cgctgcttct ctccgccact gagattgacg cctttttctt gcacattggt tttgagtccc
     2281 tggttgagcc tttctaccac aggctcaaaa cgcgcgagca ccagggctcg cttcaaatcg
     2341 acctcactgc cggcagtagc gaaactgata ttgtcctcca ccgaatcgtt gaatatctca
     2401 ggatcttgcg gcataagggt ggtacaggcc gccagatgct tcaagccata gggcaacgga
     2461 gcgccgtcgc aggaaacggc agcctcggta aagtaaatgc ctcgcaaaag actgagtaag
     2521 gtagttttgc cgctacccga cggtcccaca agggcaacgc ttttaccgcg ctccagcacg
     2581 atactcacgt cataaagatg atgttctcta tgctcatcat cttcgtagtt gaaactgaga
     2641 ttctcaacca cgatgcgtcg ccagttttcc ggcaaagccg ggcccagaac cggaagcggc
     2701 agcgcctcct gcgaccgagc ttccagcaat atggcattgg cgctttccac atcggcggcg
     2761 ttctgcacaa ccgtgccgta agtaagggca aaactgtaga aggaatcgcc gatacgccgc
     2821 aaatactcaa aaagcgtgaa gaaggtaccg gccataagca ctgcaccgcc caccagggct
     2881 tgccagacat agccggccat acaacagaca atcatgaggg taatgagaat tgtagtcagg
     2941 caccacttcc attcgttcaa ccagctaccc cgttgaaaaa ccggcagagc cgccataagt
     3001 ttttcgcgca gcgaaagaga gagtcgcttc tcaagacgca aagtaagcac ggtaacaaca
     3061 ttggagagat agtcgaacag tgccgcggca gcgcggttct cgatcaggtt ttgctccata
     3121 taaacccgcg ccaggcgccg gtcgaaataa acgatgatag ccgcagccaa agacgtcgcc
     3181 accagggcgg caacacccac ccagggcagg aagaagagca gcacaagtac tgcgccgcag
     3241 agacgaaaaa tcatatagag caagtcaaaa gattcgtcaa agaatcgcca gagtgccgtc
     3301 gacgccttat tgaccttgtc tatattatca ccggaatgat gatcgcgatg ccactgcagg
     3361 gggtgcgcaa gcaagacctc gaagagatgc attttatagt cggcgcgaat atgaaaggca
     3421 gcgtagcgct cgatcaaccg gcctggcccg tggaagagcc agaaaccaag ctgggtagcg
     3481 gcaaaaatcg caagatagac aagcacctgc tctttcagtt tcggcaaagc tccgggcgcc
     3541 gtagccgcct gcaggcagtt gagcaagcga ccaatcacat aaggctccgc cagggccaca
     3601 atttgcgcca gcaaaaagag caccaggtag agcacaataa gtggtcgcct ctgcccggca
     3661 aaatgccaac ccaagcgcat gagacgtaga accggattga tagcagccac ctctaaacaa
     3721 ccgctccatg atagcaagaa atgtgaaagg gcattctcga caaaaccgac agtggtgaac
     3781 tccagatcaa aaacgccact accggccggg aacagcggca gtcctggatt ccagctttcg
     3841 cgctccacta ttccacctag tacttttaaa caaaaaggga gagcaataga tcaggacgct
     3901 tgccaccagg gcaagtatca gaatctaaac ctctccctca aaaggaacgg cgctgaaaac
     3961 cgacagacaa ctcatgccga ttcacagcct ggagacagcc cgagtcaatc acttgggtga
     4021 ctttccggaa ctacgccatt tgcctccgaa gatgcgtttc agaaaagcct tcagccattt
     4081 ccagaaacca gttccctttt cagactctgc ctctgcttgc ttaacggcat cccggtccag
     4141 acaggtagct ccatccacgg acggaaactg cgacatgacc ctggtgaact gttcatccag
     4201 atgcgaaaca ccatagcccc gcctgtagat ttcacagatc accggaccaa tgtctttatg
     4261 ctggttttcc tcaagcttgc gctcgtattc aaaccagagc agttcgccgt agcgagcctt
     4321 ggtgccgggc acataactgg gcaggagata atcgcgatca atcagacgtt tggcaccgag
     4381 cttgagatag aactgcagtc gacgctgacg gttcttctct tcctccggac tgagaccctt
     4441 ctccttggtg gattcgatct caagaaagag gcccaggtgc tgcgggaatt tctccctcag
     4501 actgacgagc agtgcctgca tgtgcttcgt accaacacca ccggacctgc gggtctggtc
     4561 ggtagcaatg taagccaaga gagaaaagtt actcatggga gtaaccagcg agaaacagag
     4621 gagcgcgccg tctttgtccg tggtgcgatg caagagcatg cccgttgtgg aaagcagcga
     4681 ggagatttca gagaccggca tacgctcatc gacggggaag gcagcttcgt acagccttcg
     4741 ccaatcgctc tcagcgccta gaccggtgct cggtatacag agatttattt gattgttcgt
     4801 actcataaag aggagctcca aagctcatct ggaatccaaa gcacgtcaac tctcccggtt
     4861 gaattaccag gtaaagtcag tcggaatcgc ccgactacat agcgaagacc gcaatgcgac
     4921 tgcacttaac agcatcacca tagtcggtct gacaggcagt aaccaaaggg gttactggcg
     4981 agtagccgga cggggtggtg atttaaagaa aagctctgtt atttggattc aagaagatag
     5041 tgggtaaagt ggtaactcaa gttacaggca ggcgcgctct tactcaaggc ctctatcagg
     5101 cactgaaaat tgagccgccc tcgccgttct cctgacaagc ctcagaaacg agccccttcg
     5161 tcccccgaga aacagtgtgc aaaagccttt caaaatggct gagcgggtcc gaattccttg
     5221 acatccttcc ggtgactgac gaagcgactg ctcatacttg cttcttttcg ttcggcaaaa
     5281 ctcgcgctag ctcctgacta cccgggatag agggcacttc agggg
//
LOCUS       DAOQNZ010000058         5325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6279_length_5325_cov_3.559962, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000058 DAOQNZ010000000
VERSION     DAOQNZ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6279_length_5325_cov_3.559962"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2277)
                     /locus_tag="PKW73_01770"
     CDS             complement(<1..2277)
                     /locus_tag="PKW73_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72037.1"
                     /translation="MAENPELHRNRLREVNVNDFQTEDPLGALAREILNREKAAENKS
                     LLDKTVEVFYNVEKGSLENMKAVGLKAYEASKKGDTAALQAMTPTIRETIDKDRQAVQ
                     HQEEVNFYAGSFAKAVPLFAGSKSKLLLAASVGVNAAEQVKVGDDLASGSVHALMGGT
                     KGFAMKKTFDYLGPRQFFSETGVGTGTAALRPGLEYANVATKGIALGGLSRLYETGLT
                     ASNYVDAQGKVTTSSITDGLLKTTSTTLNPGAMAMDAALFMGAHGAFKQVAGAAARAT
                     ESSPLLGSVANSRLGVFSREGQVLTNAGMGATFGLATGSSQEFIRQQSSGENFDLSKI
                     ARRGVLQALTDGAAGATGSSLVHGGKMLQARAFELKTGQPQLNRGFAGDEAPLVDPTA
                     PNKAVEGDAAVQPKDGTAVVDAAPSVKDGTVVVDAASQPKEQAVVADKPVVDEGATRV
                     QPVEAAPKPESLGAGDGTSATVEVSLQADSAPTVRNPALEAISEAKAASFIGPKEPAQ
                     YVRKASYEMTEAQKPVFEEGIKLIQGVFRGHSQPGDVVKFLEFGAGRGQEVRTQFEQM
                     AKDYSELGNVQVQKLIEVAMKATPETVALAPEGVRLADQASRTGVDPLGPEYRALVEF
                     AWGAGKGAEGPVRIVSELVGHPGMNKLLGEVYAASGKLERTAEGQKPLVWGDTPTLAR
                     DQLRAILGHRPQNAEQHLVMREGIQAWADAYPAHHSVLHQYASRTRYGDIAAMIDARL
                     GTDYFSRFRNSHLTELPDA"
     gene            2575..2844
                     /locus_tag="PKW73_01775"
     CDS             2575..2844
                     /locus_tag="PKW73_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72038.1"
                     /translation="MESSKKNNLRNLQRISLKDLQALKGSDFPEEIVEKKSMLKNFLE
                     QVKQIKKEKPSLKRLLVLSKDALSSLIDQLLYETVPVKEVYLGVE"
     gene            3048..3314
                     /locus_tag="PKW73_01780"
     CDS             3048..3314
                     /locus_tag="PKW73_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72039.1"
                     /translation="MHFLRITTIEAFDLLEFQKKARETLCPKALFELWEDVCKRYERR
                     QIGEYEFEEMKEAIWPNLRALASLRRAMDEVDLPGTGDESKRSA"
     gene            3539..4753
                     /locus_tag="PKW73_01785"
     CDS             3539..4753
                     /locus_tag="PKW73_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72040.1"
                     /translation="MLSVLPGLFCLTLALTPLVSPPCARGDDNGWVDGDTEEMRERPS
                     SRRRPQTQPNYNQDRGEAVPETEGGRPQSRRKTGSSGSENFTEGDAGAGQAQPGAAAE
                     SANPVLQIKSGSNTLTGGISSSTYRRFERNNRGLDKMGSRFLQQSPYLEPPRTIAVQP
                     STFKNWLEQYHAGIAAAFSKTTIIEVKGQWDDAGHILRGYGLPFTRITSNKLKETDLA
                     QTRILVVNCGANLDAGAVQRVRDFVSGGGYLLTTDWALDSCLEKAFPGYVEWNSDYTE
                     SSIVDAVVVDEDPVLLVGVPRVAYWKLENRSQTVKVLRGREVQVLARSRDLMQRECSN
                     LGILALTFPYGAGRVLHLVGHFDNNADRAFNNALPDPAPIIGIGLRQAIAGNFLVAAL
                     SGDAKVPPAPEN"
     gene            4864..5250
                     /locus_tag="PKW73_01790"
     CDS             4864..5250
                     /locus_tag="PKW73_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72041.1"
                     /translation="MVQGNDNSFFSETPKEHVAHADSQAFQHELESMRTRDLPATAEH
                     NPAELRLTNIDTAKNLLRDDEQMSPEKFKLTQNFLKFAFSEGGFTNTDRAPVADLLQK
                     AAMNQENPAAQTGLMKMAEAIRPRTA"
BASE COUNT         1252 a         1536 c         1407 g         1130 t
ORIGIN      
        1 agcatcaggc agttcggtca gatggctgtt gcggaagcga ctgaaataat cggttcccag
       61 gcgcgcatca atcatggctg caatatcgcc gtagcgagtt ctgctggcgt actggtgtaa
      121 aacgctatgg tgggctggat aagcatcggc ccaggcttga atgccctcac gcatgaccag
      181 gtgctgttca gcattttgtg ggcgatgacc cagaatagct cgcaattggt cccgcgccag
      241 agtgggcgta tcgccccaga ccagaggctt ctgtccctca gccgttcgct caagtttgcc
      301 ggaggcggca tagacttctc ccaggagctt gttcatgccc gggtgcccca ccaactcgct
      361 aacgatgcgc accggtccct cggcgccttt tccggcaccc caggcgaatt ccaccagggc
      421 ccggtactcc ggaccaagcg gatcgacacc ggtccgggac gcttgatcgg ctaagcgcac
      481 cccttccgga gcaagtgcca ccgtttcagg agtggctttc atagccactt cgattaactt
      541 ctgaacctgc acatttccga gttcggaata gtctttagcc atttgctcga attgggtgcg
      601 cacttcctgt ccgcgtccgg caccaaattc aaggaatttg accacatcgc cgggctggga
      661 atgaccgcgg aatacaccct ggatgagctt aatgccctct tcaaaaacgg gcttctgagc
      721 ctcagtcatc tcgtagctgg ccttgcgcac atactgtgcc ggctctttgg gtccgatgaa
      781 agaggcggcc ttggcctcgg agatagcctc cagagccggg ttacgcacgg tcggtgcact
      841 atcagcctgg agagaaactt ccactgtggc ggaggttccg tcgcccgccc cgaggctctc
      901 cggtttggga gcggcttcca cgggctgcac ccgcgtagcg ccttcatcca caaccggctt
      961 gtctgcaacc acagcctgct ctttgggctg actagcggca tcgacaacca cggtaccgtc
     1021 tttgacagaa ggagcggcat cgacaactgc ggtgccgtct tttggctgca ctgcagcatc
     1081 gccttctact gccttattgg gagcggtcgg gtctaccagg ggcgcctcat caccggcaaa
     1141 gcctctgttc aattgcggtt gtccggtctt cagctcaaaa gcccttgctt gcaacatctt
     1201 gccgccatgc accagggaag agccggtagc accggcggca ccatcggtga gggcttgcaa
     1261 gacgccgcgg cgagcaattt tactcagatc aaaattctca ccgctggact gttgtctgat
     1321 gaattcctgc gaagagcctg tagccagacc aaaggtggca cccataccgg cattggtcag
     1381 aacctgccct tcgcggctga agactcccag gcgagaattg gccacggaac ctagcagcgg
     1441 tgagctttcc gtcgcacgcg ccgccgcacc tgccacttgc ttgaaggctc cgtgggcgcc
     1501 catgaagaga gcggcgtcca tagccatagc accgggattg agggtggtgg aagtggtctt
     1561 caacaagccg tcggtgattg agcttgtagt cactttgccc tgggcatcca cataattgga
     1621 tgcagtcagt ccggtttcgt agaggcgcga cagaccgccc agagcgatac ctttggttgc
     1681 tacgttggca tactccagac ccggtcgcaa agccgcggtg ccagtaccca caccagtttc
     1741 ggagaagaat tgacgagggc caagataatc gaaggtcttc ttcatggcga agcccttggt
     1801 cccgcccatg agcgcgtgca cactgcccga ggccagatca tcgccgacct tcacttgctc
     1861 ggccgcattg acgcccaccg aggcagccag aagcagctta cttttcgaac cggcgaaaag
     1921 cggcacggcc ttggcgaagc taccggcgta gaaattgacc tcttcctgat gctgtacggc
     1981 ttgtctatct ttatcgatgg tctcgcgtat ggtcggcgtc atagcctgga gggcggcagt
     2041 gtcacccttc ttagaggctt cgtaggcttt gagcccgact gccttcatat tttcgagaga
     2101 acctttttct acgttgtaga agacctccac ggtcttgtcc agcaacgact tattctcggc
     2161 tgccttttcc ctgttcaaga tttccctggc cagagcaccc agaggatctt cggtctggaa
     2221 atcatttacg ttgacttccc gcaacctatt tctatgtagt tccggattct cagccatgat
     2281 accgccagga aagacctgaa aacgccaact atatggtcag actatgcgcc attgacaacc
     2341 ctttcaatgg ggataccacg aaggtgacca gtactttttg ccctgaaagc ccgaacttca
     2401 atcgctcttg ggctcttccg ccttttcgcc gacaaaaatg aacgcctcct tttcaggctt
     2461 tcccatgagc gatttaattt tccaaagccg gcacgtgcca caaaaaaatc acaaatcttt
     2521 cctatcattc aggcatcggt taaccgagca ctggcgaagg aaatggctga gaccatggaa
     2581 agttccaaaa aaaacaattt gagaaatcta caaaggatca gtctcaagga tctgcaagca
     2641 cttaagggaa gcgacttccc ggaagaaata gtagagaaga aatccatgct caagaatttc
     2701 ctcgaacagg tcaaacagat caagaaagag aagccttcac tcaaacgact tctcgtgctc
     2761 agtaaagacg ccttaagctc cctcatagat caacttcttt acgaaacagt accggtgaaa
     2821 gaagtctatc tgggcgtcga ataagaccga acggattttc cagacaaaag gggcgggctc
     2881 aaggttttac cttgagtccg tcggggcttt ttggggcgct ccgggaagca taatcaaaac
     2941 cgcctcaagg atatccactg atgatatgtc accacccctg gtagcggtgc cggcaaagaa
     3001 taacttgaca gaatcccata aactaggcac tctagttaat aggtgttgtg cattttctga
     3061 ggattactac catcgaagcc tttgacctac tcgaatttca aaagaaagcc agggagactc
     3121 tttgccccaa ggctctcttt gagctgtggg aagacgtctg caagcgctat gagcgcaggc
     3181 agattggtga gtacgaattt gaagaaatga aagaagcaat ctggccgaac ctgagagcgc
     3241 tcgcctcttt gcgccgagcc atggatgaag tcgacctgcc cggcaccggt gacgaatcca
     3301 aaagaagcgc ctagcaatct tgatcgccat aaagcaagcg cagcagcatc cttgcaagtg
     3361 gtgtcgcccc atggttcttg ggttagcctg aattatgggc tatactgagc tgacttgagc
     3421 ttccctttgg gaaacggaac ggactgggtc tgaacgctca aaacagctca atagagcgcc
     3481 ccttgtgttg taaatcgtgc caccagagaa cttaccaacc tcagacaaac gccattcagt
     3541 gctgtcggtc ctgcccgggc tcttctgcct gaccctggcc cttacgcctc tggtcagccc
     3601 accgtgcgcc cgcggcgatg ataacggttg ggtcgacggc gacacggaag agatgcgaga
     3661 gcgcccgtcc tcaagacgca gaccgcaaac gcagccaaac tataatcagg accggggaga
     3721 agctgttccc gaaacggaag gcggtcgtcc acaatcacgc cgtaagacag gaagtagcgg
     3781 cagcgagaat tttacggagg gcgatgccgg tgcagggcaa gctcagccag gtgcggcggc
     3841 agagtccgcc aatccggttc tacagatcaa atccggcagc aatacattga ccggcggtat
     3901 cagttccagt acctaccgaa gattcgagcg caacaatcgc ggtctcgaca aaatgggttc
     3961 ccgctttctc cagcaatcac cctatctgga gccgccccgt accatcgccg tgcagccctc
     4021 cactttcaaa aactggctgg agcaatatca tgccggcatc gccgccgcat tcagcaagac
     4081 cactattatc gaagtgaaag gccagtggga cgacgccggt cacattttgc gcggctacgg
     4141 gctgccgttc actcgcatca cttccaacaa gctcaaggaa accgacctgg cccagacccg
     4201 catcctggtg gtaaactgcg gcgccaatct agacgccggc gccgtgcaga gagtgagaga
     4261 cttcgtctcc ggcggcggct acttgctgac cacagactgg gcactggatt cctgcctgga
     4321 aaaagccttt cccggttatg tggaatggaa cagcgactat acggaaagca gcattgtcga
     4381 cgccgttgtg gtggatgaag accccgtctt actggtgggt gtaccgcggg ttgcctactg
     4441 gaagctggag aatcgcagcc agacagtgaa agtattgcga ggacgagaag tgcaagtgct
     4501 ggcccgcagc cgcgatctaa tgcagagaga atgctccaat ctgggtatcc tggccctgac
     4561 ttttccttac ggcgcgggca gagttttgca cctggtcggt cacttcgaca acaatgccga
     4621 tcgcgctttc aacaatgccc tgcccgaccc tgctcccatc atcggcatcg gtctcaggca
     4681 agcaatcgcc ggcaactttc tggtggcggc cctgagtggt gacgcgaagg taccaccggc
     4741 acctgaaaat taattgtttc caaggggcgt ctggcagagc ctcttttcgg gtacaacctg
     4801 atctgataag tatgttccgg ctttcaagac ggcattaatg gcataaggcg cgcgggtaat
     4861 atcatggttc aaggcaacga caactccttc tttagcgaaa ctccgaaaga acacgtagcc
     4921 catgcagact cccaggcttt tcagcacgag cttgagtcca tgcgcactcg cgacctgcct
     4981 gctaccgcgg aacacaaccc ggctgagctc cgcctcacca acatcgacac tgctaaaaat
     5041 ctgcttcgcg atgacgagca aatgtcgccg gaaaaattca aactgacgca gaactttctc
     5101 aagttcgcct tcagcgaagg cggcttcacc aacactgacc gagcaccggt agccgatctt
     5161 ttgcagaaag cagccatgaa tcaagaaaac ccggcggcac aaacgggact tatgaaaatg
     5221 gctgaagcga ttcgcccccg cacggcataa gtacctttcc tgaaagcttc aagaaaagtc
     5281 cactgcagac acaaccgccc ttattgatgc aagggtgcgg tagcc
//
LOCUS       DAOQNZ010000059         5318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6287_length_5318_cov_4.106213, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000059 DAOQNZ010000000
VERSION     DAOQNZ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6287_length_5318_cov_4.106213"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3330)
                     /locus_tag="PKW73_01795"
     CDS             complement(<1..3330)
                     /locus_tag="PKW73_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72042.1"
                     /translation="MENGISPYIAKVSLARRLTSAIAAFIVVIALFAGSAVQAQTYVP
                     PGPGQDWGTGNSTTDLSRDANNTDPTSRYIYDQPGQTTLVREQAEQNTDAVAQNVEAK
                     LWPAFGSYNETYEGLAAFWGDDIISNFFANIGQIIGKWLSEFINGWVADAVQFLTGFL
                     RIFVLNPNIATNGLAASGGPADDISPYIRQGADIMYGIAVDLLLLLFILCIWKYWAEA
                     AWRGGGNLMGAVGRLIFTAGLMLAWPTIYAFEIQISNEMIRAIYFNSADQVAMLDAAM
                     AAAVKAGLVAGAGLLANATAPVAGQVFGGLLGAGPGGLILGTVGSLVAFVGLILYLIV
                     GGVLIAQLVYILVLKAIQTGLLCAQYMFAPIFLVFFATPDTENVTSGFVRSFVEVSLW
                     TFVWVGLLKIMVIVVLSNFNPWGKIVMAIGVLQIMIQVPSFLARAQISPMSDFISAGL
                     ITGGLLSAGKALTNGLGHRAMHLANAVGNFGYAGAKGSPKSQGVDLNVPQGVNNEELL
                     KNKREAERTGKVPGQGPDNPGGQPLNPIGPKGPKTGPDGTKLDKNGNPIPPTGKGINP
                     LTGKPDGTGVGPDGAKAAQDAGLNPNGAKGDGTNIPTPPKTGKEGTLANAAADAAKTG
                     LGTAAVAGGLTAAMAAAQAPGLTGGQTPGAKDEQGRQADLSAQQAAEALKALKEQNNA
                     GPNGVGKGAGEQQAKDLKSATGVPGGKDANSKLEGKEGAVPGTGVDAKLQEKGKDGST
                     ATNAATAAAAAAANVGKDLKPGDLANALPKGADKTGTGATGGKNAEMDVEVERDPAST
                     AGSAAAAAAALNSLKSGGTEGGVKGAGTGVTGPDATGQKTSLKAPGMEGASGQVTTDV
                     KGNLVSKPGESPNKDGKPVVPPAASAGLGDAAKNISRGAADPTTVAVNSDIAPDGDPG
                     APLQQVRGNALNTANSTGRADQSVTPGGGLDAKDVATAAGIGTAAGLAASKLTQGATT
                     VEQKQTAGGKSVTAAGGGDVTTNVSGQLNSAGQPVSGAANVVPPVGGVNVTPDGANLE
                     GADATLNPGRTAATINTTGGANQQQVTPGAGLNAGDIASTAAASAALGVAANKLTQPG
                     GSTVDARLTSQGGKVSATGVPT"
     gene            complement(3466..3891)
                     /locus_tag="PKW73_01800"
     CDS             complement(3466..3891)
                     /locus_tag="PKW73_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72043.1"
                     /translation="MPLNTESDYDEIECVGVSVQYSGSSENTQSCVESPPGPTTLVRE
                     QAEQDTYPVAQDVEAKLWPAFGSYNETYEGLAACGGYDSDSNSVFNVIVNIGQLLRHW
                     FSQWFSSLRCAYSQFTLFSQSDPNFVSQWNSGKTLVMED"
     gene            complement(4529..4957)
                     /locus_tag="PKW73_01805"
     CDS             complement(4529..4957)
                     /locus_tag="PKW73_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72044.1"
                     /translation="MTKVSSLAKKIAASHSFKVATRVGFLLAPQLVLMAVGIYDQAAH
                     AQGLVGAAVDPRYGQSNEVGMLADYGYDTARDISRVVTALSTIPSSLAGMKIAFGQRD
                     GGESAMNVAKGLGIGFGGPTAVHLLGTFAINNFGGLGAGL"
BASE COUNT         1254 a         1447 c         1457 g         1160 t
ORIGIN      
        1 cgtgggaaca ccagtggccg agacttttcc accttgactg gtcagtcgcg catcaacggt
       61 agagccgcct ggctgggtca atttattggc agccacacca agggctgcgc tggcggccgc
      121 tgtagaagct atatcgccgg cattgagacc ggctccaggc gtcacttgtt gctgattggc
      181 gccgccggtt gtgttgattg tggccgcggt tctgcccgga ttaagagtag catcggcgcc
      241 ttcaagattg gctccatccg gggtgacatt cacaccacct acgggaggaa cgacattagc
      301 agcaccactg acaggctgac cggcactgtt caactggccc gagacattgg tagtgacatc
      361 gccgccaccg gcagctgtaa cactcttacc gccggcagtc tgcttctgct caacagtcgt
      421 ggcgccttga gtcagtttgg aagccgccag accggcagcg gtaccaatac cggcagcagt
      481 ggcaacgtct ttggcatcga gtccaccgcc aggtgtaact gactgatcgg ctctgcctgt
      541 gctgttggcg gtgttcaatg cattaccgcg cacctgttgc aagggcgcac cggggtctcc
      601 atcgggagcg atatcactgt tcaccgctac ggtggtggga tcggcagcac ctctggaaat
      661 attcttagcc gcgtcaccaa gaccagcaga ggctgccggc ggcaccacgg gcttaccgtc
      721 tttgttcggg ctctcaccag gcttactgac caggttacct tttacatcgg tggtcacctg
      781 accgctggca ccctccatac cgggagcctt caacgaggtt ttctgtccgg tagcatcagg
      841 tccggtcaca ccggtgccgg cacctttcac accgccctcg gtaccaccgc tcttcagact
      901 attcaaagcc gcagcagccg ccgctgcaga accggctgtg ctggcaggat cgcgctccac
      961 ttccacgtcc atttcggcat tcttaccgcc ggtggcacca gtgcctgtct tgtcggcacc
     1021 cttcggaagc gcgttggcca ggtcgcccgg cttcaagtct ttgccgacat tggcagcggc
     1081 agccgccgcc gcagtagcag cattagtggc agtgctacca tccttacctt tttcttgcaa
     1141 cttagcatcg acaccagtgc cgggaactgc accttcctta ccttccagct tggaattggc
     1201 atccttgccg ccaggtacac cggtggctga tttcaagtct ttggcttgct gttcgccagc
     1261 gcctttaccg acaccatttg gtccggcatt attctgctct ttcagagctt tcaacgcttc
     1321 ggccgcttgt tgagcagaaa gatcggcctg acgaccctgt tcatcttttg cgcccggcgt
     1381 ctgaccgccg gtcagacctg gagcctgggc agcagccatg gcagcagtca aacctccagc
     1441 aacagcagca gtgcctagac cggtcttggc cgcatcggcg gcagcattag cgagagtacc
     1501 ttccttaccg gtcttgggag gtgtcggtat attggtgccg tcacccttgg cgccgttagg
     1561 gttgagaccg gcatcttgag cggctttcgc tccgtccggt ccgactccgg tgccgtccgg
     1621 cttaccggta agaggattga tacccttgcc ggtgggcgga atgggattgc cgtttttgtc
     1681 gagttttgtg ccgtccgggc cggtcttggg gcccttaggt ccaattggat tgaggggctg
     1741 gccgcccggg ttatcaggac cctgacccgg taccttgccg gtgcgctccg cttccctctt
     1801 gttcttgagt agttcttcgt tgttgacacc ctgaggcaca ttcagatcta cgccctggga
     1861 tttaggcgag cccttggcac cggcataacc aaagttaccg acggcattag ccaggtgcat
     1921 ggctctgtga cccaatccat tggtgagggc cttaccggca gataagagac ctccggtgat
     1981 gagaccggca gaaatgaagt cggacatggg cgaaatctga gccctggcca ggaaggatgg
     2041 tacctgaatc atgatctgga gcacgccgat ggccatgaca attttgcccc agggattgaa
     2101 gttcgagagc accacgatca ccatgatctt gagcaagccg acccagacga aggtccagag
     2161 gctcacttcc acgaaagagc gaacaaatcc ggaggtgacg ttttccgtgt cgggcgtggc
     2221 gaagaacacc aggaatattg gggcgaacat atactgggcg cagagcaagc cggtctgaat
     2281 ggctttcaat accaatatat agacgagctg ggcaatcaaa acgccgccca caatcaaata
     2341 gagtatcaag cccacaaagg ccaccaggct acctaccgta cccaggatca aaccgccggg
     2401 accggcacca agcaagccac cgaagacctg accggctacc ggcgccgtgg cattggccag
     2461 cagtccggca ccagccacca gaccggcttt gacagcggca gccatggccg catcaagcat
     2521 agccacctgg tcggcggaat tgaaatagat agcccgaatc atttcgttgg agatctgaat
     2581 ctcgaaggca tagatagtag gccaggcaag catcaaacca gcggtaaaga tcaaacgacc
     2641 aaccgcaccc atgaggttgc cgccgcctct ccaggctgct tcagcccagt acttccagat
     2701 acagagaatg aagagcagca aaagcaagtc aacggcaatg ccatacatga tgtcggcgcc
     2761 ttgacggata taaggcgata tgtcatcggc cggaccaccg gaagcggcaa gtccgttagt
     2821 ggcgatgttg ggattgagta cgaagatgcg caagaatcca gtcaggaact gaacggcatc
     2881 ggctacccaa ccgtttataa actccgacaa ccacttaccg ataatctgtc cgatattggc
     2941 aaagaagttg gaaataatgt cgtcgcccca gaaagcagcg agaccttcat atgtttcgtt
     3001 ataagaaccg aatgcaggcc agagcttggc ttccacgttc tgagcgacgg catcggtgtt
     3061 ctgttcagcc tgctcacgca ccagggtggt ctgacccggt tgatcataga tgtagcggga
     3121 ggtggggtct gtgttattgg catcccggct caaatcagtg gtgctgttac ctgtgcccca
     3181 atcctgaccg ggacctggag gcacgtaagt ctgagcctga acggcactgc ctgcaaacaa
     3241 ggcgataaca actatgaaag cagctatcgc cgacgtcaga cgccgggcca gagagacttt
     3301 agctatgtag ggtgagatgc cgttttccac tttatttcat tcttctaggc aagaaaagca
     3361 aggctcaaga ctgacttttc ggggtgtctt gtggaaacac tttaggacga aaattaatat
     3421 ctgtcagtgt ggttattccc atctagacgg gaaatctggc agtttttaat cttccatcac
     3481 taaagtttta ccactgttcc attgggagac aaaatttggg tccgattgag agaaaagagt
     3541 gaattgcgaa taagcacatc ggagtgaaga gaaccactgg gaaaaccaat gtctaagcag
     3601 ctgaccgata ttgacaataa cattgaaaac gctgttggaa tcactgtcgt agcctccgca
     3661 agcagcgaga ccttcgtaag tttcgttgta agaaccaaaa gccggccaga gcttggcttc
     3721 cacatcctga gcgacgggat acgtgtcctg ttcagcctgt tcgcgcacca gggtggtggg
     3781 accaggaggg ctttcaacgc aactctgtgt attttcagac gagcccgagt attgaacgga
     3841 aacaccgacg cactcaattt catcataatc cgattcagta ttgagcggca aacaggactg
     3901 agcctgaaag gagtatgcaa ggagcaaagc aattgccgat acatcaaaaa ccagggaaac
     3961 cgaacggcgc ctccaaagtg ccgatgcgta ggcgtgggac ctcgctccga aagacaagca
     4021 gggaccgacc aaagaaccta cccagacgcc gtcccagaca cttaagccct tccaaaatcg
     4081 ggataaattg cggttttcca ttattctcaa tttcccccgg aagaaaagca aagcacaaga
     4141 gcaccacagg gcgcacctct tgccaacaat ttaaatgcaa acaccctctc tgtcagtgtg
     4201 agaagtccca attagatggg gattccggct cagtttaatc tccaagaggg caatcggctg
     4261 gagagagata gccctcctga ttgcgacaaa tgaggcgcaa atgaaattag taataatttc
     4321 gtgcatcttg cttccaaatg accgcaatta gaacaaataa gcgaaaaacg acggaatttc
     4381 gctcacgccc ctgttttaaa aaagagacta cccaaaggca ctacggggca agagtcaatg
     4441 catacacccc cggcgcaatc gtcggcaaaa aaaacaggtc ctcttgcgag gacctgttca
     4501 attttcttac ttttgcggct agttccgatt agagaccggc gcccagacca ccgaagttgt
     4561 tgatagcgaa ggttccgagc aggtggacgg cagtaggtcc gccgaatccg ataccgagac
     4621 ctttggctac gttcattgca gattcaccac catcacgctg accgaaggcg atcttcatac
     4681 cagcgaggga ggacggaatg gtggacagag cggtgactac gcgggagatg tctctggcgg
     4741 tgtcatagcc gtagtctgcc aacataccta cttcgttcga ctgaccataa cgggggtcga
     4801 ctgcggcacc gactagaccc tgggcatgag cggcctggtc ataaataccg actgccatga
     4861 ggaccagttg aggagcgagc agaaatccca cgcgagtagc gactttgaag gagtgagaag
     4921 ctgcgatttt tttggcgagg gatgaaactt tagtcatgga ggacctccta attgggtgat
     4981 ttatagagaa agttttggtt ggtgtttgtt gattgccagc gcgccttagt gtcttacgat
     5041 agtgccacta acagaggtgg atgctccacc ggcgcctgag aacccgctcg attgcggtac
     5101 gattgatcca ggtgcgaaac ctgtatttcc gtaactttga attgcgggtt tgttgagtct
     5161 tccagcatta gcagtacggg caatgtattg cggtttgatt ggagttctca aacgaccatt
     5221 cacattttcg gatgaccgac gtggctggct cgtagacttg gccgctggca attgcggggc
     5281 agtcattgct gagggagatg cagtcatagg ctgcatgg
//
LOCUS       DAOQNZ010000060         5308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6304_length_5308_cov_3.865410, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000060 DAOQNZ010000000
VERSION     DAOQNZ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6304_length_5308_cov_3.865410"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKW73_01810"
     CDS             <1..763
                     /locus_tag="PKW73_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72045.1"
                     /translation="SLPAGRAVVLVADRTKDLLTWHRPRVLSRFGAKEVNGVKFYVPS
                     SDGKSYIPETVSQSSKMTDDLMSALSAWTEFRYADFDGCWLTDRGMKALDAHKELVEL
                     TINSPGVKSDPLKERPFLLRLKRLSLSHVEDVDKLTGTLAKVKNLDSLYLGYCTPSEA
                     LFEALQSNKSLTMLRLNKCPVDDAALAAIDGIASLKNLRLKECDGLNEALVPALSRMR
                     RNLKIDISVSENKYKNIRGAEALPNVRIHLLENPG"
     gene            complement(803..2059)
                     /locus_tag="PKW73_01815"
     CDS             complement(803..2059)
                     /locus_tag="PKW73_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNA72046.1"
                     /translation="MPRTKPANQNCCQRQLSRFAAINSKKTGTWLTLALCLAITVAEP
                     VSAASKFDQGTAAYKGGDYVKAAAFFQDAYRADNKDVNSLYYLALCHHQLRNWSGAKQ
                     IYRSICGSFPSSPAATRAREALKLLEQPAAAATAASPSGATTSNTTANDSQSAEQDPE
                     LASLPDEAHFYFTKNHHNHMEVDIYVNGHPVKAAFDTGANAFFYKDQLKAAGVDVNNA
                     VAGGTTKGWAGNTVQTQRMPASIRLGTLTRTINIFMEETTTGLGQNLIGQDFVKGYQY
                     EIDDKGNRVDLKKISNSGGKDRIDPLYDIPCELEGKRDIVTIDINGRKSKAFLDTGSS
                     KTIVDPATAKSLGLEPSGETMHWRGIGGDTEMQIAYGTVRIGPLVRENFPILIGGHIG
                     CCVGQDLMEGWRCKVDRGRKLLRFFH"
     gene            2382..3332
                     /locus_tag="PKW73_01820"
     CDS             2382..3332
                     /locus_tag="PKW73_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNA72047.1"
                     /translation="MEARFSAEDILCAASGRIVKGPIDLQKGRIVWQLDELQPGDWFL
                     ALQDGVHDGHDDLRQAIASGARGCIVNRRCRYAFADTNGVIISVSDTRSALFDLVRSW
                     RYAVKPRVVGVSGTTGRRATMLLMNSLLAEKFKVHLAFMSSLGSLGCLSEVLAMPEDT
                     ELLIFEAAAVERGDVAKVGGTLDPDLAVITRIEHPLPSGGRERLLANMYCELLETVET
                     YSGNGKAVVYDRSAAVQERTDLLLSGMASVRFSQSASDSYNCVSEEQFEDLNSTLFKL
                     TGQKLTRAELWCAIEAARSLDVTFDPASAFDGSKLLAYGV"
     gene            3342..4919
                     /locus_tag="PKW73_01825"
     CDS             3342..4919
                     /locus_tag="PKW73_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNA72048.1"
                     /translation="MYFPILQSQLLLLNCLGALSIPLTALLASIIFSRREFKLRALLA
                     GQLSALACALFALAFYMVHPQDLSFSVAGLFELKFDLLSLVLALLITFISSIVLFFSE
                     RYLVGDSRQTAFMQHLVVLTSFAAFLSASDNLILSLVCWHGISLTLWRLVGLRKGGGA
                     AQRTVLAHHVLSDAAFALSACLLVAVCGTANLTELSSRVTLLGEQVWFKSSMPVSALT
                     VASLLLVVSLSIKSALFPFHRWLLATLEAPTPLSGLLHAGVVNVSAVMAARLWPILSG
                     SSQSLLVWGLMAAVSVIVGALVMSTQSDVKRKLVFSTVSQMGFMCLQCATGAIPAAIF
                     HLLAHGLFKCHLFLQSGSAVSEGLMKRQWGQPSPADWRRPQTLMVLGCVLTMVLPVCY
                     WLLRGSSGESGAVVSCIIAAAAIGCSMPSLQRSGLFLVVVSSLAALLMIEISGTVEHW
                     LKEGLASGVLPGGGLLFYSVCAFALVGTLLHLLRDKSFGQAMYVHALNGFYVDEIVGL
                     FAGRRAAPPRPVAAEKR"
     gene            4994..>5308
                     /locus_tag="PKW73_01830"
     CDS             4994..>5308
                     /locus_tag="PKW73_01830"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNA72049.1"
                     /translation="MKVDSAARCLNFFWPLTSFIACNPLKGYEDLPFEEALRLSGRLF
                     GARGFMPLSEYRAMYESGRISPADLQEAFADGRELSSAAQGAVSEAALSTLADCLDER
                     HDT"
BASE COUNT         1020 a         1336 c         1508 g         1444 t
ORIGIN      
        1 gagtttgccg gccgggcgag ctgtagttct ggttgccgat cgcaccaagg atttgcttac
       61 ctggcacaga cccagagtat taagccggtt tggagcgaaa gaggtgaacg gggttaaatt
      121 ctacgttccc agctccgacg gcaagagcta tattccggag acagtcagtc agtcttctaa
      181 aatgaccgat gatctgatgt cggccctcag tgcttggacc gaattcagat atgctgattt
      241 tgatggttgt tggctaactg accggggaat gaaggccctt gatgcccaca aagaattggt
      301 tgaacttacc attaatagtc ctggtgtcaa aagtgatcct ctgaaggaga gacctttcct
      361 tctcaggttg aagcgtttgt cactctccca cgtggaggac gttgataagc ttacaggaac
      421 actggcaaag gtaaagaacc tggactctct ttatcttggt tattgcacgc cgtcggaagc
      481 tctcttcgag gcgctacaga gtaacaagtc tctcaccatg ctcaggctga acaaatgtcc
      541 agttgatgat gcagctcttg ccgccattga tggtattgct tccctcaaga atttgcgcct
      601 gaaggaatgt gacggactga acgaagctct ggtgccggcg ctttctcgta tgcggcgcaa
      661 cctgaagata gacatatcgg tgtccgagaa caaatacaaa aatatcaggg gggctgaagc
      721 acttcccaat gtgcgcattc acttactgga aaaccctggt tgattgcgcc atttacgcgt
      781 accagagtta gcgcccgcat gtctaatgga agaatcgcag tagtttgcga ccgcgatcaa
      841 ccttgcagcg ccagccttcc atgagatctt gcccgacgca gcagccgata tgaccgccta
      901 tgagtatggg aaagttttcg cgcaccagtg ggccgatgcg tactgtgccg taggctattt
      961 gcatttccgt gtctccgcct atacctcgcc agtgcatggt ttcgccgctg ggctccagac
     1021 ccagagactt agcggtggcc ggatcgacaa tagttttgga tgagccggta tcaagaaatg
     1081 ctttcgattt acgaccgttg atatcaatcg tgactatatc gcgcttgccc tccagttcac
     1141 agggaatgtc gtagagcgga tcgatcctat ctttgccgcc gctattactg atcttcttta
     1201 gatcgacccg gttgcctttg tcgtcgattt catattgata gcctttgaca aagtcctgtc
     1261 caatcaggtt ctgtcccagc ccggtggtgg tttcttccat gaagatgttg atggtgcgag
     1321 tcagggtgcc cagcctgatt gaggcaggca tgcgctgggt ttgcaccgtg ttgccagccc
     1381 agcccttggt ggtgccgccg gctacggcat tgtttacatc cacaccggcc gccttcaatt
     1441 ggtctttgta gaagaaggcg ttggctcccg tgtcgaaggc tgctttcacc ggatgaccat
     1501 tgacgtagat atccacttcc atgtggttgt ggtggttttt ggtaaagtaa aaatgggcct
     1561 cgtcaggcaa agaagcaagt tcagggtctt gttcggcaga ctgtgagtcg ttggcagtgg
     1621 tattagatgt agtggcgccg ctcgggctgg cggcggttgc cgcagcagcg ggttgctcca
     1681 gtaatttcag ggcttctctg gctctggtag cggccggcga tgatggaaaa gaaccgcaga
     1741 tagagcggta aatttgcttg gcgccggacc agttgcgcag ttggtggtgg caaagagcca
     1801 gatagtagag ggaattgacg tctttgttgt ctgctcggta ggcgtcttga aaaaaggcgg
     1861 cagccttgac atagtcgccg cctttatagg cggcggtgcc ctggtcgaac ttggaagcag
     1921 cgctgaccgg ctcggcgact gttatggcca aacagagggc gagagtaagc caggtgcctg
     1981 ttttcttgct gttgatagcg gcgaaccgac tcagttggcg ttgacagcag ttttgattgg
     2041 cgggtttggt gcgcggcatc tcaggagtac tttcggcaga ggtatataga cgatggaaga
     2101 gaatgtttat tttaaccgga ttggcgaaag ccttctgttt ttcacttgag atgtcggctc
     2161 gtcattcgtt tttgatggac cttaatatgt ttgcgcagct tttgcactca ggaccggcgc
     2221 ggggaagtcg gcggggatag cgcttgaata gtcggcctga tggttttgat aagaaccttg
     2281 acggacctga ttgacaggct cgtcaaattg tatatacttg catatattgg catgtgcaga
     2341 tatgcaaaga tttgcacaca taacaaggag ggattgggca gatggaagca agattctcgg
     2401 ccgaagatat tttgtgtgcc gcttcggggc gtattgtcaa aggtccaatc gatcttcaga
     2461 aaggccggat tgtctggcaa cttgacgaac tgcagccggg agactggttt ctagccctcc
     2521 aggatggtgt gcatgacggt catgacgact tgcgtcaggc tattgctagc ggtgccaggg
     2581 gctgtatagt caaccgccgc tgtcgctatg cttttgccga tactaacggc gtcatcattt
     2641 ccgtttccga caccaggtct gctctgttcg acctggtgcg ctcctggcgt tatgctgtga
     2701 aaccccgggt ggtaggtgtg tccggcacca cggggcgccg ggccactatg cttctgatga
     2761 attcgctgct ggcagaaaaa ttcaaagtgc acctggcctt catgtccagt cttgggtccc
     2821 tgggctgcct ctctgaggta ctggctatgc ccgaagatac tgaacttttg atcttcgaag
     2881 cagcagccgt tgaacggggt gatgtggcta aggtaggagg cacgttagat ccggacctgg
     2941 ccgtcatcac ccgcatcgag catccgctgc cgtccggtgg aagagagcgg ctcctggcta
     3001 acatgtattg tgagctgctt gagacagtcg aaacctactc aggcaatgga aaagccgttg
     3061 tgtacgatcg cagtgccgcg gtgcaggagc gcactgactt gcttttatca ggcatggcgt
     3121 cggttcgctt ctctcagtct gcctcagaca gctacaactg cgtttcggaa gagcaatttg
     3181 aggacttgaa cagtactttg ttcaagttga ccggacagaa gctcaccagg gccgagctat
     3241 ggtgcgccat agaagcagcg cgctccctgg atgttacttt cgatccggct tccgcctttg
     3301 acggcagcaa acttcttgcc tatggagtgt aagtcagact gatgtatttt ccaattttgc
     3361 agtcgcagtt gcttttgctt aactgcctgg gagcgctctc aataccgctt acggctctgc
     3421 ttgcttcaat tattttcagc aggcgagaat ttaaattgag ggcgctcctg gctggacaac
     3481 tgagcgcctt agcctgtgcg cttttcgctc tcgcctttta tatggtgcac cctcaagatc
     3541 tgtcattttc ggttgctggt ctattcgagc tcaaatttga tctgctcagt cttgtcctcg
     3601 ctctcctgat taccttcata tcttctatcg tgctgttttt cagcgagcgt tacctggtgg
     3661 gtgattccag gcagactgcc ttcatgcagc atctggttgt gttaaccagt tttgccgctt
     3721 tcctatctgc atccgacaat ttgattctca gccttgtttg ctggcatggt atttccctca
     3781 ccctctggcg gctggttggt ttgaggaagg gcggcggcgc cgctcaacgc actgtacttg
     3841 cacatcacgt tctcagtgat gcagcttttg ccctctctgc ctgtctgcta gtggctgtct
     3901 gcggcactgc caatcttacg gagctttcga gccgggttac tctgcttggc gagcaagtct
     3961 ggttcaagtc gtccatgccg gtttcagctc tcacagttgc gagtttgctc ctggtagttt
     4021 ccttatcgat taagtcggcc ctcttcccct ttcacagatg gcttttggcc accctggagg
     4081 ctcccactcc tctctcgggg ttgctgcatg ccggagtggt gaatgtttcg gcagtgatgg
     4141 cagcgcgctt gtggcctatt ctctccggtt cttctcagtc tcttttggtc tgggggctca
     4201 tggcggcggt ctcggtcatt gtgggggctc tggtcatgtc cactcagtcg gacgtgaagc
     4261 gcaagctggt cttctctaca gtcagtcaga tgggcttcat gtgcttgcag tgtgctacgg
     4321 gagccatccc tgccgccatc ttccatttgc tagcccacgg tcttttcaaa tgccatctct
     4381 tcttgcagtc gggcagcgcc gtctccgaag ggcttatgaa gaggcagtgg gggcagcctt
     4441 ctcctgccga ttggagaaga ccccagactc tcatggttct cggctgcgtt ttgactatgg
     4501 ttctgcccgt ctgttattgg cttttgagag gcagctccgg agaaagtggt gcggtggtct
     4561 cctgcataat tgccgctgct gccatcggct gctctatgcc ttccctgcag cgctccggct
     4621 tgttccttgt agtggtctcc agtctggctg ctctgttaat gatcgagata tccggtacgg
     4681 tggagcactg gcttaaggaa ggtctggcat caggggtact gcctggtgga gggctactct
     4741 tctatagcgt gtgtgccttc gccctcgtgg gcaccctgct tcacctgttg cgtgacaaga
     4801 gctttgggca ggctatgtat gtgcatgcct tgaacggttt ttatgtggat gaaatcgtcg
     4861 gactcttcgc cggtcggcgt gcggcaccac cgcggccggt cgcggcggaa aagcggtagc
     4921 gtcgttattt tgtatattcg aaaacaaagg ggtgagagca ttgaattcta ttgattcaaa
     4981 actgagttta gagatgaaag ttgattccgc cgccaggtgc ctcaatttct tctggccgct
     5041 caccagtttt attgcctgta acccattgaa gggctatgaa gatttgccct ttgaggaagc
     5101 acttcgtctg tccgggcggc tcttcggtgc ccgaggtttc atgccgcttt cggagtaccg
     5161 ggctatgtat gagtcgggac ggatcagccc tgccgacctg caggaagcat ttgccgacgg
     5221 tcgtgagctt tcctctgccg cccaaggcgc cgtgtccgag gccgcgctct ctacccttgc
     5281 cgactgcctt gatgaacgcc acgacacg
//
LOCUS       DAOQNZ010000061         5276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6377_length_5276_cov_3.707719, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000061 DAOQNZ010000000
VERSION     DAOQNZ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6377_length_5276_cov_3.707719"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKW73_01835"
     CDS             complement(<1..784)
                     /locus_tag="PKW73_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72050.1"
                     /translation="MHGKLDLGKAKGLKQSELKKLNRLLQKRIPKDKILTVELADSIA
                     EITHETGYPLSVVVNRRGQVVNVTVGQPSEVDMPELRGVRVGPGRLCGHRIIHTVLPH
                     NLKDADEPGRAGKNPHPQIAYSKENLQFMARNRLDLLAQIAVDPAGSFSRSRGEQQKH
                     ADQVLLAHLLPGRDPEGKLWKILPPMTARGTQEDNFEELISSLEEEFRTRAPELAVAE
                     GEERAFLVGLVCDGANSWQVEDDLDELARLARTAGATVCGRLT"
     gene            complement(1083..2870)
                     /locus_tag="PKW73_01840"
     CDS             complement(1083..2870)
                     /locus_tag="PKW73_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNA72051.1"
                     /translation="MADSPFDSKSGLRPPAKPESEAGPPGGKKPLAGDSLFSMLAMNS
                     ARAQEAARAKPVSSEPKPKGGGVAAYGADFLASVVVFLVALPLCMGIAIASGLSPAHG
                     IVTGIIGGIVVGFLGGSPLQVSGPAAGLAVVVLELIKEHGTERLGVIFCLAGIVQVIA
                     GLCRLAPWFRAVPPSVINGMLSGIGVLIFAAQFHVMVDDSPKGSGINNLLSIPAAIWK
                     GLNVPGTGAAETTHHLAAAVGLVTIVILVLWQRFAYGRLKLLPGSLVAILLATIFSAT
                     LALPVRYVNIPDNMFASLHITSLLDFQKYMSTEVLLDAVAIAFIATAETLLTCAALDK
                     MHSGPRTNYDRELVAQGVGNTLCGMVGCLPLTGVMVRSGVNVAAGAKSRLSSVLHGFW
                     ILIFVVVLPHILELIPTSALAALLVFTGYKMVNFRVIKDMRRYGRSEALIYLATVVLI
                     VCQDLLTGVIAGVVFSVCKLLYIFSHLEIKLRYDPKRNRTHVSLSGAATFLSLPKLAD
                     TVDRVKPDSELHIHLEDLEYIDHACLDMLMNWDKQHRASGGTLVMDWGALGTVFRERR
                     KGTRGENREFRAEPVDEKPAADGEANGSA"
     gene            complement(3070..4512)
                     /locus_tag="PKW73_01845"
     CDS             complement(3070..4512)
                     /locus_tag="PKW73_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNA72052.1"
                     /translation="MVLAAKLVDALKAIVGAENVLTDAFDLSLYDADAETLDTASPDL
                     VVLPASTEEVAAVVKAVATLAPTLPVTARGAGTGLSGGATCVRGGLSLVLTRMNKVLH
                     IDAAERAALVQVGATNVSVSRQAEPHGLYFAPDPSSQIASTIGGNIAENAGGPHTLKY
                     GMTTHHVLALKGVNARGEVVALGGFNRGGLDLTGVVIGSEGTLMVVTEALLRLTPLAA
                     KVETLLAYFPTVRQCGQAVSDIVAQGVVPAAMEMIDNLTLNAVEDYLGLGLRRDAQAL
                     LIIELDGVEAGIACARAIVEKCLHENGLLESRWAENARERADIWKARKTSFGALGRIA
                     PHGYVLDGVIPRSRLAEAIEGIAKIGERHGLTIANVYHAGDGNLHPCMLYHREDQAEV
                     ARVISAGREILHLCVELGGTLSGEHGIGVEKLMEMESAFGPRDLEFMGLIKTSLDPDG
                     RLNPGKVLPQLKTCGESGARPLLRHQQLSC"
     gene            complement(4579..>5276)
                     /locus_tag="PKW73_01850"
     CDS             complement(4579..>5276)
                     /locus_tag="PKW73_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72053.1"
                     /translation="DNPVDQLLEAPPGSFEVETPKAAPPKKPFNLNVQDQGQGVFSGE
                     QMPGSEDTMPTTPPPVQKNPLRNGVQQTAPRNNEPDSSAEMQLLWDAWHKRVAEAIYM
                     RYNGLAQMAFKASPPLMCQVSYCVARDGRIGNVRILQKSPNPMYNAMLLGVINSMTNN
                     PVLEFPPNSRRQYVEKTGTFTWNYGNEGYKHMINDRETVQQGQPRQQQMQQPQQMQQQ
                     QMRPMQQMMPMMR"
BASE COUNT         1248 a         1522 c         1427 g         1079 t
ORIGIN      
        1 gagtgaggcg accgcaaacg gtggcaccgg cggtgcgggc caggcgggcc agttcgtcta
       61 gatcgtcctc cacttgccag gaattagccc catcgcagac cagtcccacc agaaaggccc
      121 gctcctctcc ttcagccaca gccagttcag gggcacgcgt gcgaaactct tcttccaggg
      181 aactaataag ttcctcgaaa ttatcttcct gggtgcccct ggccgtcata ggaggcagga
      241 ttttccagag ttttccttcc ggatcgcgac ctggtaaaag gtgagccagc agtacctggt
      301 cggcgtgctt ctgttgctca cctcgactgc ggctgaaact accggcggga tcaacggcaa
      361 tttgagccag aagatcgaga cgattcctgg ccataaactg cagattttct ttggaataag
      421 ctatctgtgg atgaggattc ttaccggccc gaccgggttc atcagcatct ttcaaattat
      481 gaggcaagac cgtatgaatg atgcgatggc cgcaaagcct gccgggtccg actcgcacgc
      541 ccctcagttc gggcatgtca acctcggaag gttgaccgac ggtgacgttc accacctgac
      601 cacgcctgtt caccaccact gaaagtggat agcctgtttc atgggtaatt tccgcgatgg
      661 aatcggcaag ttccaccgtc aagatcttgt ctttgggaat acgcttttgc agtaaccgat
      721 tcagcttctt tagttcagac tgcttgagtc ctttagcttt tcctaaatct agtttgccgt
      781 gcaatatacg acctccggaa gggaattttt aaggtccgac tggcggacta taacgggctg
      841 ggtagagcaa aagcgcaggc tcatgttcga atcatacccg tttatcagtc gaattacact
      901 cacaattaat gtatccagca ggtccacaaa agtgaccata agggctatct gccagggttc
      961 cggctctcgg aaagggcagg ctcttggcat tggcgaagcg gggacgatca cggcaaaggc
     1021 tcatcaatag ccgcaaagca gacggaacta aaagatcaag aacaggctat gttccaatcg
     1081 cctcaggccg agccgttcgc ttcaccatct gctgccggtt tctcatccac cggctcagct
     1141 cgaaactcgc gattttcacc cctggtgccc ttgcgccgct ctcgaaatac cgtaccgaga
     1201 gccccccaat ccataaccag ggtgccgcct gaagcacggt gctgcttgtc ccagttcata
     1261 agcatatcca ggcaggcatg gtcgatatac tcgagatctt ccaaatggat atgcaattcc
     1321 gaatcgggct tgaccctatc cacggtatcg gccaatttgg gcagactgag aaaggttgcg
     1381 gcgccactca aggagacatg ggtgcgattg cgcttaggat cgtagcgtaa cttgatctca
     1441 aggtgagaaa aaatgtagag caacttgcag acggaaaata caaccccggc gataacaccg
     1501 gtgagaaggt cctgacagac aatcaaaacg acggtggcca gatagatcag ggcttccgag
     1561 cgaccgtaac ggcgcatatc tttgatgact cggaagttga ccatcttgta gccggtaaag
     1621 acaagcagag cagccagggc agaggtgggg ataagctcaa gaatgtgagg caataccacc
     1681 acaaagatca aaatccagaa gccgtgcaag acggaagaaa gccggctttt ggctccggct
     1741 gccacattga cgccgctgcg taccatcacg ccggtgaggg gcaagcaacc gaccattccg
     1801 caaagagtat tgcccacccc ctgcgccacc agttcccggt catagttggt gcgcgggccg
     1861 ctgtgcatct tatcgagagc agcacaggtc aaaagggttt cggcagtggc aataaaggcg
     1921 atggccacgg catccagcag cacctcagtg ctcatatact tttgaaaatc gagcaggctg
     1981 gtgatgtgca aagaagcgaa catattgtcg ggaatgttga catagcgaac cggcagagcc
     2041 agcgtcgctg aaaaaatagt ggccagcaaa atcgccacca gggagcccgg caagagtttc
     2101 aggcggccat aagcaaacct ttgccagagc accagtatca caatggtgac cagacccacg
     2161 gcagccgcca gatgatgagt ggtttcagca gcacctgtac cgggcacgtt caaccccttc
     2221 cagatggcgg ctggaatgga aagtaggtta ttgataccac tgcccttggg agagtcgtca
     2281 accatgacgt ggaattgagc ggcaaaaata agcacgccga tgcccgacaa cataccgttg
     2341 atcacgctcg gcggtacggc ccgaaaccag ggagcgaggc ggcacaaccc ggcaatgacc
     2401 tgcacgatac cggccaggca gaagataaca cccagcctct cggtgccatg ctctttaata
     2461 agttccagca caaccacagc cagaccggca gccggaccgc tcacctgcag tgggctgcca
     2521 cccagaaagc cgactacgat accgccgata ataccagtaa cgataccgtg ggcggggctg
     2581 agcccggagg caatggcaat acccatacat agcggcaggg caactagaaa taccaccacc
     2641 gaagcaagaa aatctgcgcc ataagcggcc acgccgccac ccttcggctt cggctcggaa
     2701 gaaacgggct tagcccgggc tgcttcctgg gcacgggcac tgttcatggc cagcatggaa
     2761 aagagagaat ctcccgccag aggtttctta ccgccaggcg gccctgcctc cgactccggc
     2821 ttagccggcg ggcgcaagcc tgacttggaa tcaaagggtg aatcagccac ggcttaaaca
     2881 aacctcttag aagggtcttg cacctgatgc cattccagat tagcaaccgg aggcttttgt
     2941 tcccggcagg ctaaataaag ccatcaaggt cgactacggc cctcagtctg gaaatctgat
     3001 ataggtcaat tttagcagcc gggaaaggag aaacactaaa aaaatcccat ccagcggagt
     3061 ctgaacaagt caacaggaga gctgctgatg tcgcaaaagc gggcgtgcac cggactctcc
     3121 gcaggttttc aactgaggca gcaccttgcc tggattgagt cgcccgtccg ggtccaggct
     3181 ggttttaatc aaacccatga attccaggtc ccgggggccg aaggcggact ccatttccat
     3241 aagcttctcc acgccgatac cgtgttcacc agaaagggtg ccgcccaatt ccacgcaaag
     3301 atgcaagatt tctctgccgg cgcttattac cctggccacc tccgcctggt cttcccggtg
     3361 gtagagcata catggatgca ggttgccgtc accggcatga taaacattgg caatggtcag
     3421 tccgtggcgc tcgccgattt tggctatgcc ttctatggct tcagccaggc gagatctggg
     3481 aatgacgccg tcgagtacat aaccatgggg agcaatgcgc cccagcgccc caaaggatgt
     3541 cttgcgtgcc ttccagatat cggcccgctc cctggcgttc tcggcccagc gactctccag
     3601 cagcccattt tcgtgcaaac acttttccac aatggctctg gcgcaggcaa tgccggcctc
     3661 cactccatcc aattcgatta tcaaaagggc ctgggcatcg cgcctcaaac ccaggcctaa
     3721 ataatcctca acggcattga gggtgagatt atcgatcatt tccatagcag ccggcaccac
     3781 accctgcgcc actatatccg ataccgcctg gccgcactgg cgcacggtcg ggaagtaggc
     3841 caggagggtc tcaaccttag cggccagcgg tgtcagccgc aaaagggcct cggtcaccac
     3901 catcaaggta ccctcggaac caataaccac accggtcaga tcgagcccgc cccggttgaa
     3961 accacccaga gcgactactt ccccccgggc attgacgccc ttgagagcca gcacatggtg
     4021 agtggtcata ccgtacttaa gtgtatgggg tccaccggca ttttcagcga tgttgccgcc
     4081 aatggtggag gcaatttgag aactgggatc gggagcaaaa tagaggccat gaggttcagc
     4141 ctgcctggaa accgaaacat tggtagcccc tacctgcacc agggccgccc gctctgcggc
     4201 atcgatgtgc aagactttat tcatgcgggt gagcaccaga ctgagcccgc cccgcacaca
     4261 ggtggcaccg ccggaaagcc cggtgccggc tccccttgcc gtcaccggca aagtcggggc
     4321 cagtgtcgcc accgccttca ccaccgccgc cacttcttca gtggaagctg gcaagaccac
     4381 cagatcaggg ctggccgtat cgagggtctc ggcatcagca tcatagagac tgaggtcgaa
     4441 ggcatcggtg agtacattct cagcaccgac aatggccttt agagcatcaa caagtttagc
     4501 ggcgagaacc atacacacct gaaattgagg gcatggctat tctaatgcat ttttcgggtc
     4561 agggtcggcc gccagccgct agcgcatcat gggcatcatt tgctgcatgg gtcgcatttg
     4621 ctgctgttgc atttgctgtg gttgctgcat ctgctgctgc cggggctgac cctgttgtac
     4681 agtctcccgg tcgttgatca tatgcttgta gccttcgttg ccgtagttcc aggtaaaagt
     4741 accggttttt tccacatact ggcgcctgga attgggcgga aattccagca ccggattatt
     4801 ggtcatggaa ttgatcactc ccagaagcat ggcgttgtac atgggattgg gacttttctg
     4861 caaaatacgc acattgccta tgcggccatc tctagccacg caatagctga cctgacacat
     4921 gaggggcggg ctggccttga aagccatctg agccagaccg ttgtaacgca tgtaaatagc
     4981 ttcggccacc cgcttgtgcc aggcgtccca gagcagctgc atttcagccg aactgtcggg
     5041 ctcgttgttt ctcggcgccg tctgctgcac accgttgcgc aggggatttt tctgcaccgg
     5101 cggcggcgta gtgggcatcg tgtcttccga gccgggcatt tgctcgccgc tgaaaacccc
     5161 ctgtccttga tcttgtacat ttaagttaaa aggtttcttg ggcggcgccg ccttgggcgt
     5221 ctcaacctcg aaagaaccgg gaggcgcttc cagcaactga tctaccggat tgtctt
//
LOCUS       DAOQNZ010000062         5272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6386_length_5272_cov_3.593061, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000062 DAOQNZ010000000
VERSION     DAOQNZ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6386_length_5272_cov_3.593061"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1616)
                     /gene="metG"
                     /locus_tag="PKW73_01855"
     CDS             complement(72..1616)
                     /gene="metG"
                     /locus_tag="PKW73_01855"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNA72054.1"
                     /translation="MKKFYVTTAIDYVNAAPHIGHAYEKIAADVVARYYRLRGFDVFF
                     LTGVDEHGSKVEKSAQAAGKTPIEFCDEISSTFEKSWRNLNLSFDYFIRTTEERHAKV
                     VQSLFTKLKEQGDIYKSTYMGLYCEGCEDFLRERDLDDNGHCPTHKKAPRELAEENYF
                     FKLSKYKPAIKQWLEENPDAVRPEGRRKEVLNQLNDPELTDFSVSRSRKSLSWGIPIP
                     GDDDQVIYVWMDALTNYITGVGYLSDEKQFERYWPADLHLIGKDITKFHSIYWIAFLL
                     AANLPLPKQIYAHGFITVEGQKISKSLGNVIDPNKLVEEFGADAVRFFLMSGTSFDQD
                     GDFSKHEFIRKVNSELANNLGNLVNRTLTLIDKNLDGKIPDCKPEHDLREKANTLHTV
                     FDSCMEKLEFAKAIEAVMATVDQTNKYMNDEKPWTQFKEGKVKEGSEVLYTAVEMLRR
                     VAFQIYPFAPKMASDIWWQLGHDDTLEVIGDSEKPDGFFDMIPVGQKVRNTGPIFKRL
                     EEPNAP"
     gene            1769..3040
                     /gene="coaBC"
                     /locus_tag="PKW73_01860"
     CDS             1769..3040
                     /gene="coaBC"
                     /locus_tag="PKW73_01860"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNA72055.1"
                     /translation="MKMKKGSKKRGSNFESFNPLENKTILLGVSGGIAAYKACDIASL
                     LRRSGASVHAVLTPNAREFITPLSLTTITRNQTHCEQYGPTADWRPEHIDLAKKCDLI
                     LIAPATADIIAKLATGICDDLLTTMVLASQAKVMIAPAMNPVMLQHPATQHNLSILEN
                     RYRYDIVAPEYGEVACGDVGLGKMAAVESIIAAVAARLLAHQTLSTKRVLVTAGGTRE
                     PIDPVRFIGNRSSGKMGIAMADSAYSRGADVTLISTVDVERAYPVIVVETAQEMQEAV
                     EAEFDSCDALVMTAAVADFRPLAVSAQKIKKTESEDIVLELTKNPDIIDLLGRVKRKD
                     QVIIGFAAESEALLSNAASKLKRKNLDMIVGNDISVPDIGFGSDDNAVVILSAEGTRE
                     SLPRMPKRAIADHICDLLSERFQVLEKELAH"
     gene            3153..3539
                     /locus_tag="PKW73_01865"
     CDS             3153..3539
                     /locus_tag="PKW73_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNA72056.1"
                     /translation="MGRADNYSYGDLPARRDDEDHGGGWGAWMTGLLIGAVGGATLAL
                     LTAPKSGSEIRGSLERTAKNVPDRVGEVVDDSLDLYASFLNYCQLTLEEQTLRFKRAI
                     AAGKLAAAKKREEIELGGGQLPFQHR"
     gene            3603..4034
                     /locus_tag="PKW73_01870"
     CDS             3603..4034
                     /locus_tag="PKW73_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72057.1"
                     /translation="MDQISGSLNILVAVSLIFLAVSLMAVSMPLMSMVKQSERTLSAL
                     ERLLGTVDKELGPTLKQVDTLLGTVIELKGRAERSIGDVGNKVGDVTGGLTKAADSAK
                     KNSSVFGAGFMAGIRAYLEPKGNKEERERSRDARSLTEVGK"
     gene            4112..4447
                     /locus_tag="PKW73_01875"
     CDS             4112..4447
                     /locus_tag="PKW73_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNA72058.1"
                     /translation="MSRFLEGLVVGGAIGYIIGLLSAPKSGAELRRQIADGSEDLYKQ
                     ASDSLTDIKEKTGTTITDLQSKSNEVLKKASDSVAGTKDQIAHKLQELAGQGGKVLVD
                     DVESAASGG"
     gene            4456..5172
                     /locus_tag="PKW73_01880"
     CDS             4456..5172
                     /locus_tag="PKW73_01880"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNA72059.1"
                     /translation="MPTLYRLNRAIALTGIASRRKADELIAAGKVMVNGTLTTDFSLL
                     VDIDQDQIVVDGEEVVTRFHDYLLLNKPKDYVSTCDDERGRRTVLDLLPPELRHLKPV
                     GRLDRDSEGLILLTNDGELAKLLTHPSHDVAKIYEVTVKGQMQASDYSALAKGIRLSD
                     GVTRPAKVRKISANSHATTFEMQLYEGRNRQIRRMCAALGYDVNRLVRVAIGGLQLRQ
                     LPSGKWRRLTDSEISTLYRS"
BASE COUNT         1226 a         1310 c         1461 g         1275 t
ORIGIN      
        1 actgcgtcgt cccgaccagg taccagccct gaactcataa acagtgctct cataaacagt
       61 gctaaggact atcagggcgc gttaggctct tccaggcgct tgaagatcgg tccggtgttg
      121 cgtacttttt gaccaaccgg aatcatatcg aagaagccgt ccggtttctc cgagtcgcct
      181 atgacttcca gggtatcgtc atgacccagt tgccaccaga tgtcggaagc catcttggga
      241 gcaaagggat aaatctgaaa agccacccgt ctgagcatct ctacggctgt ataaagaact
      301 tccgagcctt ccttgacctt gccttctttg aactgggtcc agggtttttc gtcgttcatg
      361 tacttgttgg tttgatcgac agtggccata acagcttcga tggccttggc gaactccagc
      421 ttttccatgc agctatcgaa aaccgtgtgc aaggtattgg ctttttctct cagatcatgt
      481 tcgggtttgc agtcgggaat tttgccgtcc agattcttgt cgatgagagt gagagtgcgg
      541 ttcaccagat tgcccagatt attggctagc tctgagttga ccttacggat gaattcgtgt
      601 ttgctgaaat cgccgtcttg atcaaaagaa gtgccgctca taaggaagaa gcgcacggca
      661 tcggcgccga actcttccac cagtttgttt ggatcgatga cattacccag agacttgctt
      721 atcttctgac cttccacggt gatgaagccg tgggcataga tttgcttggg cagaggcaga
      781 ttagctgcca gcaagaaggc gatccaataa atggagtgga acttcgtgat gtctttgccg
      841 atcaagtgca gatctgccgg ccagtagcgc tcaaactgct tctcgtcgct gagatagccg
      901 acgccggtaa tatagttggt gagggcgtcc atccagacat agataacctg gtcgtcgtca
      961 ccgggaatgg gaatgcccca ggagagcgac ttccgtgagc gtgacacgga aaaatccgtc
     1021 agttcgggat cgttgagttg attgagaact tctttacggc gtccttccgg gcgcacagca
     1081 tcagggtttt cttccaacca ttgtttgatg gccggtttgt attttgagag tttgaagaag
     1141 tagttttctt ccgccagttc ccgcggtgct ttcttatggg tagggcagtg accgttgtcg
     1201 tccagatcgc gctctctcag gaagtcttcg cagccctcgc agtagagacc catataggtc
     1261 gatttgtaga tgtcgccttg ttctttcaat tttgtgaaca ggctctgcac cactttagcg
     1321 tggcgctctt cggtggtacg aataaaatag tcgaaggaca agttgagatt gcgccaggat
     1381 ttttcgaagg tggaggaaat ttcgtcgcag aattcaattg gtgttttacc ggctgcctga
     1441 gccgatttct ctaccttgga tccgtgttcg tccacgccgg tcaggaagaa aacgtcgaag
     1501 cccctgaggc gatagtaacg agccacgaca tcggcggcga ttttctcgta ggcgtggccg
     1561 atatggggag cggcattgac gtaatcaatg gcggttgtga catagaactt tttcattgat
     1621 atggtaccgg tccttggatc tctgacgtgg gtaaaggggc tacaataagg gacgtcggtc
     1681 cataataata cagaccgccg cttattctaa ggcttgtaag gttatttggc cggggctatc
     1741 tctttgtaag gtggttttgg agaggcatat gaaaatgaaa aagggttcca agaagcgcgg
     1801 aagcaatttc gagtccttca atccacttga gaacaagacc attttgcttg gtgtgagcgg
     1861 tggcatcgcc gcttacaaag cttgcgacat cgcctccctg ctgaggcgct ccggtgccag
     1921 tgtgcacgcg gttttgacgc ccaatgcccg ggaattcatc acgccccttt ccctcaccac
     1981 catcacccgc aatcagaccc attgcgaaca gtacgggcca actgccgact ggcgccccga
     2041 gcacatcgat ctggccaaga aatgcgactt gattctaata gcgccggcca ctgccgacat
     2101 tattgccaag ctggccaccg gtatctgcga tgatttattg actaccatgg tgctggcttc
     2161 ccaggcgaaa gtgatgattg ctccggccat gaatccggtc atgttgcagc atccggctac
     2221 ccagcacaat ctctccattc ttgagaatcg ctaccgctac gatatcgttg ccccggagta
     2281 cggagaagtg gcgtgcggcg atgtcgggct tggcaaaatg gctgccgtgg aatcaatcat
     2341 tgcggcagtg gccgccaggc tcttagccca ccagaccctc tccaccaagc gcgtcctggt
     2401 gacggccggc ggcaccagag agcccatcga cccggtgcgc ttcatcggta accgctcctc
     2461 cggaaaaatg ggaattgcca tggccgattc agcatacagc cgtggtgccg atgtcaccct
     2521 catctccacc gtagacgtgg agcgcgccta tccggtgatt gtggtggaaa ccgctcagga
     2581 gatgcaggaa gccgtagaag cggaattcga cagttgcgat gccctggtta tgacggcagc
     2641 cgtggctgat ttcagaccgc tggcggtttc ggcccagaag atcaaaaaga cagaatcaga
     2701 agacattgtc ctggaattga ctaagaatcc cgatatcatc gacttgcttg ggcgagtcaa
     2761 gcgcaaagat caagtgatca tcggtttcgc ggccgaatcc gaggcgctcc tctccaacgc
     2821 cgcttccaag ctgaagcgta aaaacctcga tatgatcgtc ggcaacgata tttcggtgcc
     2881 ggacatcggc ttcgggtccg atgataatgc ggttgtcatt ctctcggcgg aaggtaccag
     2941 ggagagcctg ccccgcatgc ccaagcgagc cattgccgac catatttgcg acctgctctc
     3001 ggagcgtttt caggtacttg agaaggagtt ggcgcactaa gtcttttgca acattttgtg
     3061 ccgccaaatc ttagacaaaa gtgcacaaaa ccggttagtt tgagagcttg gtgctatact
     3121 ccagtgtttg gataggggac ggagagaagt tcatgggcag agccgataac tatagctatg
     3181 gagaccttcc tgctcgtcgt gatgacgaag accacggtgg tggttggggc gcctggatga
     3241 ccgggcttct gatcggcgcg gtaggcggag caacgctggc acttttgacc gcacccaagt
     3301 cgggcagtga aatccgcggt tccctggagc gcaccgccaa gaatgtgcct gatcgtgtgg
     3361 gcgaagtggt tgatgattcc ctcgatcttt acgcttcctt cttgaattat tgccagctca
     3421 ccctggaaga gcagaccttg cgcttcaagc gtgcaattgc cgccggcaaa ctggctgccg
     3481 ccaagaagcg cgaagaaatc gagctgggcg gcggtcaact gccttttcag catcgttaag
     3541 ccgggacgcc tggctggtcg ttcgtggtga caatacggct ctcttgttag aaggattttc
     3601 ccgtggatca aatatccggt agcttgaaca tacttgtggc cgtctccctc attttcctgg
     3661 ctgtgtcact gatggctgtc agtatgccgc tcatgtccat ggtcaagcaa tctgagcgca
     3721 cgctttcggc tctcgagcgc cttctcggca ctgtagacaa ggagcttgga ccgactctca
     3781 aacaggtaga taccctgctt ggtaccgtaa tcgaattgaa gggacgggcg gagagatcta
     3841 tcggtgatgt cggtaacaaa gttggtgatg taacaggcgg tctgacaaag gccgcagact
     3901 cggctaagaa gaattcatcg gtatttggtg ccggcttcat ggccggtatc agggcttatc
     3961 tagagccaaa gggtaacaag gaagaaagag agcgcagtcg ggatgcaaga tctttgactg
     4021 aggtgggcaa gtaattgccg gagggcaaat ccaagcaagt aagcaaaact taaatggttt
     4081 ggccaaagaa ataagaggta aaacaagcat catgagtaga tttctggaag gtctagtagt
     4141 cggcggagcg attggttata tcatcggtct gctttcagct cccaagtccg gtgcggaatt
     4201 gcgacgtcaa atagccgatg gttctgaaga cctttacaag caggcttccg actctttgac
     4261 cgatatcaaa gagaagaccg gcactaccat caccgatttg caatccaaga gcaacgaagt
     4321 cttaaagaaa gccagcgact ccgtggctgg aaccaaagac cagatcgccc acaaactgca
     4381 agaactggca ggacagggcg gtaaagtgct ggtggatgac gtcgagagcg ctgcctccgg
     4441 cggctagctt acaagttgcc cacactttat cgtctcaatc gagccattgc cctgactggt
     4501 attgccagtc ggcgtaaggc tgatgagttg attgccgccg gtaaggtgat ggtgaacggc
     4561 actttgacca cagacttcag tcttctggtt gatatagatc aagatcaaat agtcgtggac
     4621 ggcgaggaag ttgtgactcg cttccacgac tatctgcttt tgaacaagcc caaagactat
     4681 gtctccacct gtgatgatga gagaggtcgg cgcaccgtgc tggatctttt gccgccggag
     4741 ttgaggcatt tgaagccggt aggtcgcctc gaccgtgatt cagaggggct tatcctcctc
     4801 acaaatgacg gtgaactggc taaactcctc acccatccct cccacgacgt ggccaaaata
     4861 tacgaggtga ctgtcaaggg gcaaatgcaa gcttctgatt attccgctct ggcaaaaggc
     4921 attcggttgt cagatggtgt tacgcggccg gcaaaagttc gcaaaatttc tgctaattct
     4981 catgccacaa cctttgaaat gcagctctac gaagggcgaa accggcaaat caggcgtatg
     5041 tgtgccgctc ttggttatga tgttaatcgt ttggtcaggg ttgccattgg tgggttacaa
     5101 ttgaggcagt tgccgtctgg aaaatggcgt cgcctgactg atagtgagat atccactctg
     5161 tatcggtctt gaggtaaatc tggaaaaccg attttataag gaccggtcat cgcggtgagc
     5221 actgccacaa tgtctttaga gcaagtaggt cagaaactaa aagcagcacg gg
//
LOCUS       DAOQNZ010000063         5258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6422_length_5258_cov_3.901787, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000063 DAOQNZ010000000
VERSION     DAOQNZ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6422_length_5258_cov_3.901787"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..244)
                     /locus_tag="PKW73_01885"
     CDS             complement(92..244)
                     /locus_tag="PKW73_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72060.1"
                     /translation="MHKESVMAQQKGMKRAAKVAKRKVKKAAGREAAAMKKLEKQVAD
                     LVKAGS"
     gene            complement(289..705)
                     /locus_tag="PKW73_01890"
     CDS             complement(289..705)
                     /locus_tag="PKW73_01890"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA72061.1"
                     /translation="MIVVTEDEKILLVRHRKGTRQYWVLPGGRLEYGETFHECAVREI
                     KEETGLDVEVDDFVFLSEAIAPDRSRHIVNIYLTAHVTGGVLKVGDEPVLAGVDYLPL
                     ADLDKLTLFPPVGRMVIESLPKVSRGSIKYLGNLWV"
     gene            1011..2045
                     /gene="pheS"
                     /locus_tag="PKW73_01895"
     CDS             1011..2045
                     /gene="pheS"
                     /locus_tag="PKW73_01895"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNA72062.1"
                     /translation="MQDLEKKIVEMESSALKAIEEASSLDHLEQLRLSVLGQKGELTG
                     ILRGLSSLDKDVRPRIGQLANQVKVKIEQAHLDRKDRLFQSHIEARLSQERIDVTAPG
                     QTMPVGHMHPLSQIRQEILSIFSSMGFVTADGPEIETDYYNFDALNTPASHPARDEQD
                     TFYTNIGANVLLRSQTSTVQIRVMEMSKPPLRIIAPGRVYRNEEVNARKYPLFHQVEG
                     LLVDKDVTFGQLKGTLTAFIQALFKKQVKTRFRPDFFPFTEPSAELDSECPFCNCKGC
                     KTCGGRGWLELLGCGMVDPNVLSGVGIDPEEWSGFAFGMGLERLAMLKYGVNDIRHFY
                     TNDLRFLEQF"
     gene            2065..4653
                     /gene="pheT"
                     /locus_tag="PKW73_01900"
     CDS             2065..4653
                     /gene="pheT"
                     /locus_tag="PKW73_01900"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNA72063.1"
                     /translation="MKVSLDWLGEYVDLTGLKPEEIAEKLTMGAFEVEEITVFGVDLE
                     GPIVAGEIMEIHPHPNADKIRLTRTRIKDGAEPLEIVCGAQNIEVGQIVPVALPGAKV
                     VNRHDGSPLIIKASAIRGVKSNGMLCSAAELGIVCDDAAGEGILILARTSDKEKPSFA
                     LGDDVRQILNILPDYILHVEPRSNRGDALSVAGLAREAAALFGRPLKRPDWELAAAEP
                     GTISSGITGTFIENTEDCPYFSLIPISGVKIGPSHPRIAKRLESIGLRPVSNVVDITN
                     YVMHELGQPLHAYDADKLQGKALGVRRAAPGETITTLDGKERKLTDEVLLIVDSHGQS
                     DIPVGIAGIMGGKDSEISDSSTSLVLEAACFTPAVVRRGSRLLGLSSDASLRFERGVD
                     AASVLNAARRAAYLIGKYCGTQDLKIGAPHLAGSDRVQEVAVDLRLKTIKRILDLDMD
                     GKRVATLLSPLGFQVEEAGPGTLKVQVPSFRQTDVKREIDLVEEVCRLNGYDAIEASM
                     PSGTVCRENEDDTLSKAHSTLTGLGLSEAWLSSLVPADDAGIVQDYLVQVLNPLSKDH
                     QYLRQSLVPGLLGALAYNLDRGQKTVWLYETGRVYFKDYLKTTETNPGKNGKNGKTET
                     TGSEKPALEMQRLAGVLCGSGPQGACTFQTVKGVLECLLTSLGLNLSLEKVNWSTGSQ
                     LPPWLHPYRTAVLSLNRPPRKGSDLTEAVGVFGNPMVLAYVGQLHPAYGANRGLKEEA
                     FIFEIDLDRLKAERKPPRFSELANTPAVTRDLTCDFPADQAATLQHQTMARLITKKAG
                     SNLKKLELVSIYQPQGEAGGGNVAITYRLTFQHVSETLTSEQIDASLKDVKESLAGEL
                     KATFRA"
     gene            4848..>5258
                     /locus_tag="PKW73_01905"
     CDS             4848..>5258
                     /locus_tag="PKW73_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72064.1"
                     /translation="MFRMKFRSNFVGIGALVVGALLTGSMQFNPMFDGKEFLYDLPAM
                     YVAPGIIAVPFLFALAHYRSCPAVQQFSAFLVTASFISLLGLVGNFTPEKMVLIKENW
                     LLHFNVIQLSCINIAAIIAAMKIMAIEQMSSFADT"
BASE COUNT         1298 a         1474 c         1352 g         1134 t
ORIGIN      
        1 gctaacaaac cgcctgaaat tttaagcact gaaaaaagcg gcttcggatt taacttccgg
       61 cgccgctggt tttgactgct aagagcccgg gctaggagcc ggcttttacc agatcagcta
      121 cttgtttttc cagttttttc atggctgcag cctcgcggcc ggcggctttc ttcactttac
      181 gcttggctac tttggcagct cttttcatac ccttttgctg ggccatgaca ctctccttgt
      241 gcatatactt cgcccccata ctaccagatc aggtcgggag ccaagctttt acacccacaa
      301 attgccaaga tactttatgc ttccccgact gaccttgggc agggattcaa tgaccatgcg
      361 ccccacgggc gggaatagag tcaacttgtc aaggtccgcc aggggcagat agtccactcc
      421 ggccagaacc ggttcgtcgc cgactttcag gacgccgccg gtgacatggg cggtgaggta
      481 aatattaacg atatgtcggg aacggtccgg tgcaatggcc tccgaaagga agacaaagtc
      541 atccacttcc acgtcaagac cggtttcttc cttgatttcc cgcactgcac attcatgaaa
      601 agtctcgccg tattccagcc gcccaccggg caaaacccag tactgccggg tgcctttgcg
      661 atgacggacc agcaaaatct tctcgtcttc agtgacaaca atcactgaaa ctctagtggt
      721 tggaatcgtt tgtaagtttc gattacgctt aaatcttgtt cttgccaaag ctctgcactt
      781 gactcccgac gccgcctttt ccgcacattc taaggcaagt cgccgcacaa ataaatatat
      841 actaacgcct ccagcggagt gagacacaga actggtgccg ctcatgtaat ttcgcaaaga
      901 tggcaatacc cgaaagggga gaagtattat cggtctgcta tctttgagag tcgatctact
      961 ggtagctcgc ctaaaaagat ataagagcgc cccaaaggca agagagaaag atgcaggacc
     1021 tcgaaaagaa aatagtggag atggaaagct cggctttgaa ggccattgaa gaagcaagtt
     1081 ccctcgatca cctggaacag ctgagactct cggttcttgg ccaaaaggga gaactgacgg
     1141 gaatcttgcg gggtctctcc agcctcgaca aggatgtcag accccgtatc ggccaactgg
     1201 ccaaccaggt caaggtcaaa atcgaacagg cccacctcga tcgcaaagac agactctttc
     1261 aaagccacat cgaggccaga ttaagccagg aacgtatcga tgtcaccgcc cccggccaga
     1321 ccatgccggt aggccatatg caccctttga gccagattcg ccaggaaatt ctcagcattt
     1381 tctccagcat gggcttcgtc accgccgacg gtccggaaat tgaaaccgac tactacaatt
     1441 tcgacgcgct caacacaccg gcttcacacc cggccaggga tgagcaggat accttctaca
     1501 ccaatattgg tgccaacgtg cttctgcgca gtcaaacttc aacggtgcaa atccgtgtca
     1561 tggagatgag caagccgccc ttgagaatta tcgctcccgg tcgcgtttat cgaaacgaag
     1621 aagttaacgc ccgcaagtac ccgctcttcc atcaagtgga aggtctcttg gtggacaaag
     1681 acgtcacttt cggtcagctc aaaggcacac tcaccgcctt catccaggca ctcttcaaaa
     1741 agcaggtcaa gacccgcttc cgtcctgatt tcttcccctt caccgagccc agcgccgagt
     1801 tggacagcga atgtccgttc tgcaactgca aaggttgcaa aacctgcggc ggacggggtt
     1861 ggcttgaact tttgggctgc ggcatggttg atccgaacgt cttgagcggc gtcggcatag
     1921 atccggaaga atggtccggc ttcgcctttg gaatgggtct ggagcgactg gccatgctca
     1981 agtacggcgt caatgatatt cgccatttct ataccaacga tctgcgtttc ctcgaacaat
     2041 tttaagtagt aaggaagaag gacaatgaaa gtaagtcttg actggctggg cgaatatgtc
     2101 gatcttacag ggctcaagcc tgaggaaata gcagaaaagc taaccatggg cgcctttgaa
     2161 gtggaagaaa tcaccgtctt cggcgtcgac ctggaaggtc ctatcgtggc cggtgaaatc
     2221 atggaaattc acccgcaccc caacgccgac aaaataagat tgaccaggac tcgcatcaaa
     2281 gatggtgcag agccgctgga gattgtttgc ggcgcccaaa atattgaggt tggtcaaata
     2341 gtgccggtgg ccctgcccgg tgccaaggtg gtcaaccggc acgacggctc gccgctcatc
     2401 ataaaagctt ccgccataag aggcgtcaaa tccaacggca tgctctgctc agccgctgaa
     2461 ctggggattg tctgcgacga cgccgccggt gagggcatcc tcattctggc cagaaccagc
     2521 gacaaggaaa aaccctcctt cgcattaggc gacgatgttc ggcaaattct caacattttg
     2581 cccgattata ttttgcatgt ggaaccccgc tccaacagag gcgatgccct ctcggtcgcc
     2641 ggtctggcca gagaagcggc ggccctcttc ggccgaccgc tcaagagacc agactgggaa
     2701 ttagccgccg ccgagcccgg taccattagc agtggcatca ccggcacctt tattgaaaat
     2761 acggaagact gtccctattt cagcttgatt cccataagcg gtgtcaaaat cgggccatct
     2821 catccgcgta tagccaaacg actggaatct atcggcttgc gcccggtatc gaatgtggtg
     2881 gacatcacca actatgtaat gcacgaactg ggacaaccgc tgcacgctta cgacgccgac
     2941 aaactgcaag gcaaagccct gggtgtgcgc cgcgccgcgc ctggggaaac catcaccact
     3001 ctggacggca aggaacgcaa actgaccgac gaagtcttgc taatcgtgga cagccatggg
     3061 cagagcgata ttccagtcgg tatcgccggc atcatgggcg gcaaagattc agagattagc
     3121 gacagttcca ccagtctggt tctggaagcg gcctgcttca caccggcggt ggtgcggcgt
     3181 ggttcccgcc tcctcggtct ttcctccgat gccagcttgc gcttcgagcg aggtgtcgat
     3241 gcggcctccg tcctcaatgc cgccaggaga gccgcctacc tgatcggcaa atactgcggc
     3301 acccaagacc tcaagattgg agccccgcac ctggctggtt ccgaccgggt acaggaagta
     3361 gcggttgacc tgaggctcaa gaccatcaaa cggattctcg acctggacat ggacggcaaa
     3421 agggtggcaa ccctgctcag tccgcttggt ttccaggtgg aagaagcggg accgggcacc
     3481 ctgaaagtac aggttcccag ctttcgccag actgatgtca aacgggaaat cgacctggtg
     3541 gaagaggtct gccgtttaaa cggctacgac gccattgaag cctctatgcc atcgggcacg
     3601 gtttgccggg aaaacgaaga cgatactctc tcgaaagccc atagcaccct caccggtctg
     3661 ggactttcgg aagcctggct ctccagcctg gtaccggctg atgatgccgg tatcgtccag
     3721 gattacctgg tgcaagtatt gaaccctctc tccaaagatc accagtactt gagacagagc
     3781 ctggtgcccg gactgttggg agcgcttgcc tataacctgg accgcggaca aaagactgtc
     3841 tggctctatg aaaccggacg ggtctacttc aaagactatt tgaagaccac agaaacaaac
     3901 cctggtaaaa acggtaaaaa cggcaaaacg gaaaccaccg gcagtgaaaa accagccctg
     3961 gagatgcaaa gactggccgg cgtgctctgc ggtagcggtc cgcaaggtgc ctgtaccttt
     4021 cagacggtca agggagtgct cgagtgcctc ctgaccagcc tcggtctgaa tctctctctt
     4081 gagaaggtca attggagtac cggtagtcaa ctgccgccct ggctccatcc ttatcgcact
     4141 gccgtgctca gtctcaatcg tccgccccgc aaaggttccg acctgacgga agcagtgggt
     4201 gtcttcggca atcccatggt cctggcttat gtgggtcaac tgcatccggc ctatggagcc
     4261 aatcggggac tgaaagaaga ggccttcatt ttcgagatcg acctcgaccg tttgaaggcc
     4321 gagagaaaac caccccgttt cagcgagctg gccaatacac cggccgtcac cagagatctc
     4381 acctgtgact ttccggcgga ccaggcggca actttgcaac atcagaccat ggctcgcctg
     4441 ataacaaaga aagccggcag caacttgaag aaattggaac ttgtcagcat ttatcagcca
     4501 cagggtgaag ccggcggcgg caatgtggcc ataacctatc gcctcacctt ccagcatgtc
     4561 agtgaaaccc tgacaagtga acagatcgac gccagcctta aggacgtgaa ggaaagcctg
     4621 gccggcgagc tgaaagcaac cttcagggcc tagctacata tttagggatt gtttttgaag
     4681 aaaatcaatg cctgatgatt tagacttgaa tatcttgggc aaaccttccg gctatttaca
     4741 acagagtgag aataatcacc gaaactggca gttagatcgt ctcaaggcgg gtataaaggt
     4801 agcacccaaa acaggtctat cgaagaagaa agggaactga aacttccatg ttcagaatga
     4861 aattccgaag caactttgta ggaattggcg ctcttgtcgt gggagccctt ctgaccggct
     4921 ccatgcagtt caatcccatg ttcgacggca aagaattcct ctatgacctg ccggcaatgt
     4981 acgtggctcc cggcatcatt gccgtaccct ttctatttgc tctggcccat taccgctcct
     5041 gcccggcagt gcagcaattc agcgcctttc tggtaacggc atcctttatc agcttgctcg
     5101 gtctcgtggg taatttcacc cccgagaaaa tggttcttat caaagaaaac tggttattgc
     5161 acttcaacgt catccagctt tcctgtatca atattgccgc catcattgcc gccatgaaga
     5221 tcatggccat cgagcaaatg agtagctttg ccgatacg
//
LOCUS       DAOQNZ010000064         5256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6428_length_5256_cov_3.792540, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000064 DAOQNZ010000000
VERSION     DAOQNZ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6428_length_5256_cov_3.792540"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKW73_01910"
     CDS             complement(<1..338)
                     /locus_tag="PKW73_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72065.1"
                     /translation="MKKTFPYLSLILTLSLSLLSAPPTRAAGKESQSRSSAGMTLAMV
                     SPLKQQNATDGQVFIDKMYASADAYDDYVFEFEMTAFKHGKVVEAGRFYFKKPKLIRL
                     EETGSFRRGSV"
     gene            complement(348..1220)
                     /locus_tag="PKW73_01915"
     CDS             complement(348..1220)
                     /locus_tag="PKW73_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72066.1"
                     /translation="MHDNFLQTRQNNAGTISRPLKVESIASLSTGEKLSYVSRELADA
                     LSDSSFVDCVRQGDLKAGKEICLLLWPFVKELPDNISAVRSQLEQLPHHGCRKNLEAG
                     LKLLSQLADDERHYQNLYLNQCRLAGLSEEDLKQVVEAAESNLKRKQEPAFAPTAETS
                     FVQLTRRMRYYCRQGSPAQAVLAVITAELAATQFARAALSAFESYFMEREEQYGRESI
                     EAGLRWLRLHAKPNTRHAIWMKRLLLFTDTEAEGSEDTFNETTKNTDLAVTEILECLI
                     AVWRTPRFRQERQN"
     gene            1355..2281
                     /locus_tag="PKW73_01920"
     CDS             1355..2281
                     /locus_tag="PKW73_01920"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA72067.1"
                     /translation="MKDLVSTPLPPIAGVTQIQPELTKLRMSDGVDLFARIWKGKSGA
                     PVVLYLHGIEGHSQWFENTASYLNSRGISIIAPDRRGSGLNPRDRGNMTSYKGYLADL
                     EVILRKAAFDFVGHPIIVLGNCWGAKAASVIAQKDYKPVSGELILPIAGLVLTSPGIY
                     TKVDYGAGTKFQIAYNSLLGGETSHRKWPIPIEISMFTDNPTFQGFLSRDTLRLTEAT
                     SSFFVESFKLSRLAEKATGIEMPLLILQAGSDVIVDVEKVQGWFSKIKSGTKDMRIFP
                     DAHHTLDFDVNWFKEYTHVLAEWILARTPVVT"
     gene            2248..3504
                     /locus_tag="PKW73_01925"
     CDS             2248..3504
                     /locus_tag="PKW73_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNA72068.1"
                     /translation="MDTGSDSRGHLMQIKSIVSRPVRLPFRFSFGHSLASRNFSRNIV
                     VEVAIAVGEGTVTGLGESVPREYVTGETSESALAFINEVLAPALLGRSFAGVDQLLGA
                     LEALFYQERLDERPLGAAFCAVELALLDACARILGLPFYLMPAYLARLSRLPAAASTL
                     DRTLWNLELPWSERVFRYGGVVPFGKRKVLEALLRAYKAYGFQTVKLKVGDDIDEDLV
                     KIKLARSILGPAVKLRIDANCAWNFDLACLAMERFRPLGVVSLEQPLPRLETEGLARL
                     QARLPETIVVDESLTTLKEAQRLIELKACRAFNIRISKVGGIVAAMKLVELARQASLE
                     CHLGAQVGESGILASAQKHLALAVPDLFANVEGAMNLFLLKRDLTRECLTVPFGAVVS
                     ARPPERANLGLAVNLKRESLLKLMEA"
     gene            3513..5228
                     /locus_tag="PKW73_01930"
     CDS             3513..5228
                     /locus_tag="PKW73_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNA72069.1"
                     /translation="MSDWKNLDVIAGQYLRRATHVQRFYNGLKSCAKTQEEKLLSIVR
                     RNRNSAFGKAHNFDRINSVEDYRRLVPPSNYEYFEPYIEELKRGKGNQLTCQEPFMFA
                     TTSGTTARPKFVPITPLHVEDYTHAFQVHNYHLVKDMPGGALGKFLIITSNDEEGRTE
                     GGLPYGAVSGMLNKRQSPVIRRHFALPFELCKVKDVDAKYYLLLRAALVQNVTAMLAC
                     NPSSLLLLADQMKERAESLLADIHDGTINKAFVSQVPSYILDAFAPYLKPSPERAGAL
                     LKLIEEHGRLKPCHVFPDLAVLSCWKGGPMGFYLEQMSDFYGHLKIRDFGYMASEGRG
                     TVPISSEGAGGPLAVTSHFFEFVHEDDIDKDNKRFYMAHELKPGLRYFIFFTTNAGLY
                     RYNINDLMLVESMLFQTPVLSFVRKGGGVSSVTGEKITEEQVLSALRTTVAALSLSEI
                     KHFTAEAVLDRPPHYQCYAEVEQGSERIAFAEDFQEDFLNCFDAQLKTMNPEYADKRD
                     SKRLGRPRLKLLAPGTYARLRQQRVMEGAPEAQVKIPLLSPSGFSSRLSSIAAATSLT
                     GGGLA"
BASE COUNT         1099 a         1370 c         1466 g         1321 t
ORIGIN      
        1 accgagccac ggcggaaact gccggtctcc tccaggcgaa tcagtttggg tttcttgaag
       61 tagaaacggc cggcctccac cactttgccg tgtttgaagg cggtcatttc aaattcgaac
      121 acgtagtcat cataggcgtc ggcgctggca tacatcttgt cgatgaatac ctgaccgtcc
      181 gtggcgtttt gctgtttcaa ggggctgacc atggctagag tcatgccggc gctgctcctg
      241 ctttgacttt ccttaccggc cgctcttgtt ggcggtgccg aaagaagaga gagggagagg
      301 gtgaggatga ggctaaggta ggggaaggtt tttttcactt gtcttactca gttttgtctt
      361 tcttgacgaa agcggggggt tctccagaca gcaatcaagc attcaagtat ttccgttacg
      421 gccaggtctg tatttttagt ggtttcatta aaagtatctt cactaccttc tgcttcagta
      481 tctgtgaaca gaagcaggcg cttcatccat atggcatgtc tggtgtttgg tttggcgtgc
      541 aggcgcagcc accttaagcc ggcctcgata gactcccgtc cgtactgctc ttctctttcc
      601 atgaagtagc tttcgaaggc actgagggcg gctctggcga actgggtggc ggctaactcc
      661 gccgttatga cggccagaac agcctgggcg ggactgccct ggcggcagta ataccgcatg
      721 cgcctggtca gttgcacaaa actggtttct gccgtgggcg caaaggctgg ctcctgcttt
      781 ctctttaaat tagattcagc cgcttccaca acctgtttca gatcttcctc gctcaagccg
      841 gccagtcggc attgattcaa ataaaggttt tgatagtggc gctcatcatc ggcgagctgg
      901 ctgagtaact tcaaaccggc ctcgagattt ttgcggcagc cgtggtgcgg taactgctcc
      961 agttgggagc gcacggcgga tatattgtcg ggcagttctt tcacgaaagg ccagagcaaa
     1021 agacaaattt ctttaccagc tttcaggtcg ccctggcgaa cacagtccac aaaggagctg
     1081 tcactgaggg catcagccag ttctctggac acataggaga gcttctcgcc ggtggaaaga
     1141 gaggcgatgc tttccacttt caggggtcta ctgatggtgc cggcattgtt ttgcctggtt
     1201 tgcaaaaaat tgtcgtgcaa aagttcttcc tcgcgggttc gcccgataac cggcggcgaa
     1261 ttttatactt tactacgtag cacccttgct tttccgagac ctgccaggat gttaaacttt
     1321 tcaccagtcc tgcctatacc ccttgagaca ccccttgaaa gatctcgtat ccacgcccct
     1381 gccgcccata gctggagtga ctcagattca gccggaactg accaagctcc gcatgtccga
     1441 tggggttgac ctttttgccc gaatttggaa aggcaagagc ggtgctccgg ttgtgcttta
     1501 cttacatggc attgaggggc acagtcagtg gtttgagaac actgccagct atctgaatag
     1561 ccgtgggatt tccattattg cccccgacag gcggggctcc ggtttgaatc cccgggaccg
     1621 cggcaacatg acaagctaca agggctacct ggccgatctg gaagtcatct tgcggaaagc
     1681 cgctttcgac tttgtcgggc atcccataat cgttctgggc aattgctggg gggcgaaagc
     1741 agccagcgtc attgctcaaa aagattacaa acctgtaagc ggcgagctga ttttgccgat
     1801 tgccgggctg gtacttacca gcccggggat atatacaaaa gttgattacg gcgccggaac
     1861 aaagtttcag attgcttaca attccctgct tggcggtgaa acctctcacc gtaagtggcc
     1921 gattcccatt gaaatatcca tgttcacaga caatccgacc tttcagggct ttctcagtcg
     1981 ggacacgctg cgcctgaccg aggccacctc gtccttcttt gtggagtcct tcaagctttc
     2041 ccgcctggct gaaaaagcca ccggcattga gatgccgctt ctcattttgc aggccggttc
     2101 cgacgtaatc gttgatgtgg aaaaggtcca gggttggttc agcaagatta agtccggcac
     2161 caaagatatg agaattttcc cggacgcgca tcataccctg gattttgacg tcaactggtt
     2221 caaggaatac acccatgtgc tggctgaatg gatactggct cggactcccg tggtcacctg
     2281 atgcaaatta agagcattgt ctcccgcccg gtgagattgc cctttcgttt ctctttcggt
     2341 cacagtttgg ccagccgcaa tttttcacga aatattgtcg tggaagtagc gattgccgtg
     2401 ggggagggca cggtcaccgg actgggcgag tctgtgccca gagagtatgt aaccggtgaa
     2461 acttctgagt cggccctcgc ttttatcaat gaagttttgg caccggccct tctggggcgc
     2521 tcctttgcgg gagtcgatca gctcctggga gctctggaag ccctcttcta ccaggaacgc
     2581 ctggatgaaa gaccgctggg ggctgcgttt tgcgcagtgg agctggctct tctcgatgcc
     2641 tgtgccagga ttctgggttt gcctttctat ttgatgcctg cctacctggc tcgtctcagc
     2701 cggcttcccg ccgccgcctc cactctggac cggacgctct ggaacctgga gctaccctgg
     2761 agtgaacgcg tttttcgcta cggcggggtg gtgcctttcg gtaagcgaaa ggtgcttgag
     2821 gccttgttga gagcctataa agcctatggc ttccagaccg tgaaattgaa ggttggtgat
     2881 gatatcgatg aagatctggt caagatcaag ctggctcgct ccatcctcgg gccggcggtg
     2941 aaactgcgca tagacgccaa ttgcgcctgg aatttcgact tggcgtgcct ggccatggag
     3001 cgctttcgac ctctgggggt agtttcgctg gagcagccat tgccgcgcct ggagacggag
     3061 ggattggccc ggctgcaggc gcgcttaccg gaaacaatag tcgttgacga gtcccttacc
     3121 accctcaagg aggcgcaaag gctgatcgag ctaaaggctt gccgggcttt caatataaga
     3181 atttctaagg tgggtggcat tgtggctgca atgaagctgg ttgagctggc ccgccaggct
     3241 tctttagaat gtcatctggg cgcgcaggtg ggtgagtctg gtattctcgc cagtgcgcag
     3301 aagcatctgg cccttgccgt gcctgatctg tttgccaatg tggaaggggc gatgaatctc
     3361 tttcttctca aaagagacct gaccagagag tgtctgaccg taccctttgg tgccgtggtg
     3421 agcgctcgcc cacccgagcg ggcaaacctg ggtctggcag tgaatttgaa gcgagagtcc
     3481 ctgttgaaat tgatggaagc atgaccttga aagtgtcgga ttggaagaac ctagatgtta
     3541 tagccgggca gtatttgcga cgtgctactc atgtgcagcg cttttacaac ggactgaaaa
     3601 gttgtgctaa gactcaggaa gagaagctgc tttccatcgt gcgccgcaat cgaaactcgg
     3661 ctttcggcaa ggcccataat tttgaccgca tcaacagcgt ggaagattac aggcggctgg
     3721 tgccgccttc caactatgag tatttcgagc cttatatcga ggaattgaag cggggcaagg
     3781 gtaaccaact gacttgccag gaacctttta tgtttgccac tactagtggt actaccgcca
     3841 ggcctaagtt cgtaccgatc actcccttac acgtggagga ctacacccat gccttccagg
     3901 tccacaatta tcatctggtc aaagatatgc cggggggcgc tctcggtaaa tttctgatta
     3961 ttacctccaa tgatgaggaa ggtcgcaccg agggcggttt gccctatggc gctgtttcgg
     4021 gcatgctcaa taagcgtcag tcgcctgtga ttcgtcggca tttcgccctg ccttttgaac
     4081 tgtgcaaggt caaggatgtt gatgcgaagt attatttgct cttacgtgcg gccctggttc
     4141 agaacgtcac ggccatgttg gcttgcaacc cgtcgagctt gctattgctt gccgatcaga
     4201 tgaaggagcg cgccgagtca ttgcttgccg atatacatga cggcaccatc aacaaagctt
     4261 ttgtgtctca ggtaccgtct tatattctgg atgccttcgc accatatttg aagccttctc
     4321 cggagcgcgc cggggccttg cttaaattaa ttgaggaaca cggtcgactt aagccctgcc
     4381 atgtcttccc ggatctggcc gtcttgtctt gctggaaggg cggacccatg ggtttttatc
     4441 tggagcagat gtccgatttt tatggacact tgaagatacg tgacttcggc tatatggctt
     4501 cggaaggacg gggcaccgtg cctatctcct cagaaggagc gggtggaccg ctggctgtca
     4561 cttcccactt cttcgaattc gtgcacgagg atgatatcga taaagacaat aagcgctttt
     4621 atatggctca tgaattgaag cccggactga gatatttcat cttctttacc accaatgccg
     4681 gactctatcg ctataacatc aacgacttga tgctggtgga gtcgatgctc tttcagacgc
     4741 ccgtgctcag ttttgtacga aaaggcggtg gggtgagctc cgtcaccggc gagaaaatca
     4801 ccgaggagca ggttctatcg gcgctgcgta ccacggtggc tgcgttgagt ctgtccgaaa
     4861 tcaagcactt cacagccgag gctgttctgg acagaccgcc tcactaccag tgttatgccg
     4921 aagtggaaca gggcagcgag cgaatagcct ttgccgaaga ctttcaagag gacttcctca
     4981 actgtttcga tgctcagctt aaaaccatga atcccgagta cgccgacaaa cgcgacagta
     5041 agcgccttgg ccgtccccgc ctgaagctgc ttgcgcctgg tacctatgcc cgcttgagac
     5101 aacagagggt gatggaaggg gcgccggaag cgcaggtgaa gatacccctt ctcagtccct
     5161 ccggcttcag cagtcggctt agttccatcg ccgctgccac ttccctcacc gggggaggcc
     5221 tggcatgaac ggcgctttga aagtaggcgt gagcgg
//
LOCUS       DAOQNZ010000065         5245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6448_length_5245_cov_3.558574, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000065 DAOQNZ010000000
VERSION     DAOQNZ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6448_length_5245_cov_3.558574"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKW73_01935"
     CDS             <1..369
                     /locus_tag="PKW73_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72070.1"
                     /translation="ASPSEKPDYFQIFSIQPGNVKLGKVNGSAFELKIAPTSAASPED
                     KYAPLMSAIPPGKEAAVYLKLAQYFREQYIQTRKGGSTNIQAEVFKKYAMDCFDRAEK
                     LDSKSPAIKERQSFVQESRK"
     gene            complement(430..1764)
                     /locus_tag="PKW73_01940"
     CDS             complement(430..1764)
                     /locus_tag="PKW73_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72071.1"
                     /translation="MSVSARAIAKANGTAPDLARREELRKQYWFAQKYGECDEISVIR
                     PFLSEANCFIVYEPFFRGIPLNSPLLAEIPFSTVNLKLDIDCDIHTDLCAAKQEIEEA
                     LTAELANSGITVTSEPQKHKLVFKCTPHKPHLWKLVLWKEEDGVEKKNHSAPILSRKL
                     AVEHLCSNYTEKVEFLPKIRELAGLLPSYYHELKEGKPKILVDAQAKVATNVRVTFDG
                     YTVSRFPKDQRKVESSVEITSGEQLTGLSLATIEKTIRETLADQGIETTNSTSAIKNL
                     KMPTYRFKLTLSKQEGNENGAMHLFIDMQSPYLERERDGAKALWRINQNGSGYIVYNG
                     QGVLSCLREFIANMPNPFEDRTVRRTIEIPKDYQPIQLEKELIHQRNAEHGSFEDLVQ
                     PAKTAEPGTNQIREQSRIISPPVDLELLKKQPSEPNSDFKILEPYIRERKRP"
     gene            complement(1852..2481)
                     /locus_tag="PKW73_01945"
     CDS             complement(1852..2481)
                     /locus_tag="PKW73_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72072.1"
                     /translation="MYKFNYLLSALVAVIFLQQGAGAADVAPEDGNALKSALFREAKN
                     EVDYPRLSKVAGYAIRVSVEKLDLPPSKSALNKGKTRQVMEKYLKEFGLKTTREMITG
                     RSTKLSLTVTKAGTTKVNAKAKAQKSTHILKVMLFAPPAPNWKFNTRAAIMSSKIPLK
                     LSADKSDELTPILKETALKISKQYFTARSGDYEKKQEAPAPKDNPVKQD"
     gene            complement(2546..3211)
                     /locus_tag="PKW73_01950"
     CDS             complement(2546..3211)
                     /locus_tag="PKW73_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72073.1"
                     /translation="MERQREGNTKLSLAFMLAGILGTVLIPSVFADCKDCPKSESAGR
                     REGFNQPLILSGIKSIKVAVGEIDDTLKEKGANQEHLANRLKEELSTTHATIVDAGEA
                     CCKHKAGNKAADNSPILYLKVRSVSDDAKVCVYSINLSLVEKSRVARNHKEIMASVWS
                     KDLIGKVQADAGAELDGKLDLLMKEFRRDYLLANSADIAHPDMPDDAGGKNAHKKPRY
                     IRK"
     gene            complement(3307..4119)
                     /locus_tag="PKW73_01955"
     CDS             complement(3307..4119)
                     /locus_tag="PKW73_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72074.1"
                     /translation="MSTMPKPSAKAKATKRKDPTPLAAADLYCLLTGFGPFGSAKSNP
                     SELVTLSFPDIFKTGDAKTDTSSTRRAVAKQIHISKLSLDTVGATAWKTLKKSLKKLE
                     KDLEEAGKAGPVIVLMTGLASGSRALHLERFGMNLRDYRIADQAGAQVEDKPVQAEGP
                     DLLRTSLKLTAVKKSLIDAGYPCQISNHAGTFVCNELYYQVLYHLSRHRAVKACLFVH
                     MPELKDFAEATAALKRKQTARQAAAARTETARLALLRDAMLKVLEEVAKQVH"
     gene            4146..4233
                     /locus_tag="PKW73_01960"
     tRNA            4146..4233
                     /locus_tag="PKW73_01960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4179..4181,aa:Leu,seq:caa)
     gene            complement(4430..5137)
                     /gene="pdeM"
                     /locus_tag="PKW73_01965"
     CDS             complement(4430..5137)
                     /gene="pdeM"
                     /locus_tag="PKW73_01965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HNA72075.1"
                     /translation="MAEFLNFKRQGETITVLPDKAVFLPSRKTLLVSDIHLGKGQAFR
                     ARQFFAPAGVCAADLQRLLQVVTAYRAERLIILGDLVHSRDGINEELDRLFLDFRAKT
                     KNVETILVLGNHDLRVNFSSKWRLSLVNGGFEEDGFFFEHGDEDTKAQINENNKTNRS
                     GLFRFSGHIHPAVRLHGAGKASERMPCFWLRSRERCLVLPSFGRLTGAFTVSPQTSDR
                     VFIPLYDEGNILEVTLK"
BASE COUNT         1101 a         1272 c         1346 g         1526 t
ORIGIN      
        1 gcctctcctt ctgagaagcc cgattatttc cagatctttt ccatccagcc cggcaatgtc
       61 aaactgggca aggtcaacgg ttccgctttc gaactgaaga tagcacccac tagtgccgct
      121 tctcctgaag acaagtatgc tcctcttatg tcagccattc cgccgggcaa agaggcggct
      181 gtatacttga aactggctca gtatttccgc gaacaataca tccagacccg gaaaggcggt
      241 tccaccaata ttcaagctga agtatttaag aagtacgcca tggactgctt tgatcgtgct
      301 gaaaagctcg attccaaaag ccctgcgatt aaagagcgac agtcattcgt gcaggagagt
      361 cggaagtaga gccggacagt ttgcctttgc agtagcgttt gtgcaatctt taatatgcgc
      421 cgttaaacat cagggtctct tcctttcgcg gatataaggc tcaagaatct taaagtcact
      481 gttcggctca ctcggttgtt tctttaatag ttcgaggtcc acaggggggc ttatgattct
      541 cgattgctcc cttatttggt ttgtgccggg ttctgccgtt tttgccggct ggaccaggtc
      601 ttcaaaggat ccgtgttcgg catttctctg gtgaatcaat tctttttcca gttgaatagg
      661 ttggtagtct ttcggtattt ctattgtgcg tctgactgtt ctgtcttcaa atgggttggg
      721 catgttggca atgaattcgc gcaggcagct tagtaccccc tgtccgttgt aaacgatgta
      781 accactgccg ttctggttga tgcgccagag ggctttagcg ccgtctcttt ctcgctccag
      841 gtagggagat tgcatgtcaa taaaaagatg catggcacca ttttcgttac cttcttgctt
      901 gctcaaagtg agcttgaatc ggtaggtggg catcttcaaa ttcttaattg ccgatgtgga
      961 attcgtggtt tcgatgccct ggtccgccag tgtttcgcga atggttttct caatagtggc
     1021 aaggctcaga ccggtcagct gttcaccgct ggtaatttct actgatgatt ccacttttcg
     1081 ctggtctttt ggaaacctgc tgaccgtata accatcgaat gtgactctca cattagtcgc
     1141 aactttagct tgggcatcca ccagaatttt tggcttgcct tccttgagct cgtgatagta
     1201 actgggcagt aaacccgcca gttctctaat tttgggcagg aactcaactt tctctgtgta
     1261 attggagcat aaatgctcta cggccagctt ccggctcagt ataggagcgg aatgattctt
     1321 tttctctact ccatcttcct ctttccagag caccaatttc cagagatggg gtttatgcgg
     1381 cgtgcatttg aaaacaagct tatgcttttg cggctcactt gttaccgtga tgcctgagtt
     1441 ggctagttca gccgtgagag cttcttctat ctcttgtttg gcggcacaga ggtcagtatg
     1501 tatgtcgcag tctatgtcga gttttagatt gactgtgctg aaaggtattt ccgctaacag
     1561 cggcgaattt aaaggaatgc ctcggaagaa tggttcgtag acgatgaagc aattagcttc
     1621 acttaaaaag ggcctgatga ccgagatttc atcgcattcg ccgtacttct gcgcgaacca
     1681 gtattgcttt ctgagttcct ctcggcgggc gagatcaggg gcggtgccgt tcgcctttgc
     1741 tatcgccctg gcggaaacgg acatccacaa tgtctgcgcc aggcacagaa tactaaggag
     1801 agccgatata cggatcaatc ggactggaat ttgcttgttc agacctttgc ctcagtcttg
     1861 tttgacagga ttgtctttgg gggccggggc ttcctgtttc ttttcatagt cgccgctgcg
     1921 agctgtgaaa tattgcttcg agatttttag cgcagtctct ttcaagatag gcgttagctc
     1981 atcactttta tcggcggaga gcttaagcgg aatctttgaa ctcattatgg ctgctctggt
     2041 gttaaatttc cagttgggag caggtggtgc aaagagcatg acttttaata tatgcgtaga
     2101 tttctgagct tttgctttgg cgttcacctt tgtcgtgcct gctttggtga cggtcaagct
     2161 gagctttgtg cttcttcccg taatcatttc tcgagtggtt ttgagtccga attctttcag
     2221 atacttctcc attacctgtc tggtcttgcc cttgttaagc gcactcttgc tcggaggcag
     2281 gtccaacttc tcgacgctga ctctaattgc atagcctgcc actttcgaga ggcgaggata
     2341 gtcgacttcg tttttggcct ctctaaataa ggctgactta agtgcattgc cgtcttccgg
     2401 cgctacatca gccgccccgg ccccttgctg tagaaagatt acagccacaa gcgccgagag
     2461 cagataattg aatttgtaca tgggcatttt cttgactttg cttctagtgt ggaggcggga
     2521 tcctgtttga ggtgttaaga gtggtttatt tcctgatgta gcgcggcttc ttatgcgcgt
     2581 tcttgccgcc ggcatcatcg ggcatgtccg ggtgcgcaat gtcggcacta ttggccagta
     2641 gatagtcgcg gcggaactct ttcatgagca aatcgagttt gccgtcaagc tccgcaccgg
     2701 catcggcctg tactttgccg atcaggtctt tactccagac tgaagccata atttctttgt
     2761 ggttgcgagc tacgcgagac ttttctacta gagaaaggtt tatcgagtaa acgcaaactt
     2821 ttgcatcgtc actgaccgag cgcaccttca ggtagagaat cggtgagttg tcggcggctt
     2881 tattgccggc tttatgtttg cagcaagctt cgccggcatc gacgatggtg gcatgggtgg
     2941 tgctgagttc ctctttcagg cggttggcca ggtgctcttg attggcgcct ttctctttca
     3001 gagtgtcgtc gatttcgccc accgccacct tgatgctttt gatgcctgat aagattaagg
     3061 gctggttgaa accctcacgt cttccggcac tttcactctt ggggcaatct ttgcagtcgg
     3121 caaagacgga aggaatgagc actgtgccga gtataccggc cagcataaag gccaacgaca
     3181 acttggtgtt accttctctt tgtctttcca tacttttctt ttgcccttgt ttgagtttac
     3241 cgagctattg gctgccgacc tgtgcatttt agctgaattg cccgggcgaa ttattagatt
     3301 ggcgcttcaa tgcacctgtt tcgccacttc ttccaatacc ttcagcatgg catcccgaag
     3361 cagcgccagg cgtgcagttt ccgtacgggc agcggcggct tgcctggctg tttgctttct
     3421 cttgagtgcg gctgtggcct cggcaaagtc cttcaattca ggcatatgca caaaaaggca
     3481 ggcttttact gctctatgcc tgctcaagtg atagagcacc tggtaataaa gttcgttgca
     3541 gacgaaagta ccggcatggt tggaaatctg gcaggggtag cctgcgtcaa tgagggactt
     3601 cttgacggcc gtcagtttca ggctggtgcg caagaggtca gggccttcag cctgtaccgg
     3661 cttgtcttcc acctgggcgc cggcctggtc ggcaatgcga tagtcgcgca gattcatgcc
     3721 aaaacgttcc aggtggaggg cccggctccc tgatgccaga ccggtcataa gaacaataac
     3781 cggtccggct ttacccgctt cttccaggtc cttttcaagc ttcttcaatg acttcttgag
     3841 ggttttccag gctgtggctc ccaccgtgtc gaggctcagt ttagagatgt gaatttgctt
     3901 tgcaaccgcc cgtcttgtcg aggacgtgtc ggttttggcg tcgccggttt tgaagatgtc
     3961 aggaaatgac agggtaacaa gctcgctggg gttgcttttc gcggagccga agggaccaaa
     4021 gccggtgagc aggcaataga ggtcggctgc tgccaagggg gttggatctt tgcgtttggt
     4081 tgcttttgct ttagctgagg gtttaggcat ggtggacata gtgtaagata tatttccttc
     4141 cgcaagccga agtggcgaaa tggcagacgc agctgactca aaatcagccg gagcaaacac
     4201 tccgtgtcgg ttcgactccg accttcggca ccaagtttcg agaaaatgcc taccctgatt
     4261 ttggggtagg catttggctt tctgggacta ttgtgggagg ttaatggtgc atttgaggtt
     4321 ggcttgcttc tcttcttgtt tatagttctc tctatgctga ctttagtttt cgcctctgtc
     4381 cctggaatat tgcggacagt tcagtttctc aaaggggaag cttatctgct tatttaagag
     4441 taacctctag aatgttgcct tcatcgtaga gcggaataaa gacccggtcg gatgtttggg
     4501 gactaactgt gaaagcaccg gttaatctgc caaaggatgg caaaaccagg cagcgttctc
     4561 tagagcgaag ccagaagcag ggcattcttt cgctcgcctt gccggcacca tgcagtcgca
     4621 ccgccggatg gatatgaccg gaaaagcgaa atagaccgga cctatttgtt ttattgttct
     4681 cgttaatttg ggccttagta tcttcgtcgc catgttcgaa gaaaaaaccg tcttcttcaa
     4741 agccgccgtt cactagagat agtctccact tactgctgaa attcactctc agatcgtggt
     4801 ttcccagcac aagtatggtc tctacatttt ttgtcttggc tctgaagtca aggaagagcc
     4861 gatcaagttc ctcattgatt ccgtctctgc tgtgcactaa gtctcctaga ataatcaggc
     4921 gctcggccct ataggcggtg acaacctgta acaagcgctg caagtcggcg gcacaaacac
     4981 cggctggagc gaagaactgc ctggctctaa atgcctggcc ttttccaaga tgaatatcag
     5041 aaacaagcaa ggtcttgcga ctgggaagga agaccgcttt atcaggcaaa acagttattg
     5101 tttcaccctg cctcttgaaa ttgagaaact cagccataat agcgcccggc ctgcatttcc
     5161 ctggccatgt ccacttccat tctttcaatg cggctgctca gtttttccga agacagctta
     5221 ccccgcaaac gatcaaccat cagtg
//
LOCUS       DAOQNZ010000066         5229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6475_length_5229_cov_3.371086, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000066 DAOQNZ010000000
VERSION     DAOQNZ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6475_length_5229_cov_3.371086"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PKW73_01970"
     CDS             <1..1510
                     /locus_tag="PKW73_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNA72076.1"
                     /translation="MVGPSRQVGHRLRDLMKDGAAALSSFVDCGSKTVVIVGGGMAGL
                     SAGWWLKKNGIEDFLILELEPQVGGNSSFGENAVSKFPWGAHYVPLANSESKYVRQLF
                     LELGIIEADHDKEKPRYNELYLCHDPQERLFKDGSFQEGLVPKRGLKPSEEAEMKRFF
                     DTVHELRDVKGSDGKPAFAIPLDHSSEDPRFRDLDKISFARWLQENNFVSRPLNWYVN
                     YCCRDDYGATAENVSAWAGLHYFAGRRGHAANAETNSVVTWPEGNGFLVSRLKELLAD
                     KIVTNAPVLRLTEDKEGGSLCYFTRDGAVSRYKTKFTIFCAPRFLAPYMVEKYKLEKK
                     PTYAPWLVANITLKKAPAGRGVGLAWDNVSYYGDSLGYVVANHQNITTRRSSVVVTYY
                     LPLSQLPPDAARRKLLNSPEKAWSAAIVDDLEAMHPGITEDIISIDLWPWGHGMISPG
                     VDYIWQERRKLPLNQGAVHFAHSDMSGISNFEEAQYQGVQAAERVLTQLEKS"
     gene            1512..2627
                     /locus_tag="PKW73_01975"
     CDS             1512..2627
                     /locus_tag="PKW73_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72077.1"
                     /translation="MESTHSLPLERKQRQPWLSSPGFDLLFILSPALISSTFALLFGR
                     RFEGMENLPLFAWVVLVLMVDVAHVYATLFRTYLDKEALAANKRLLLVIPLACQVVGV
                     LLYSLDALYFWRALAYLAVFHFVRQQYGFLALYGRGDAQVPTWARHLDQTCIYTVTLY
                     PILYWHAHAPRHFNWFVEGDFFSASALASAILPLAGVFYILVAIAYFIKECLLFKNNG
                     FVNLPRNLLMLGTALSWYVGIVATNSDLIFTMTNVLSHGIPYMALIWLYRKGGGAGSA
                     DAGAAGSGVLQVLVSFAPAFFAFLVFLAYLEEGFWDGFVWREHLTFFAPFAGLPVISD
                     PAFLAVLVPLLSLPQSTHYVLDGYIWRVKDRNSQWSA"
     gene            complement(3076..4620)
                     /locus_tag="PKW73_01980"
     CDS             complement(3076..4620)
                     /locus_tag="PKW73_01980"
                     /inference="COORDINATES: protein motif:HMM:NF017485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA72078.1"
                     /translation="MTDLSPAQTAAFDELIKALPIGNIFVVWSHTGMGKTTILKELHG
                     LKGGKFLTMTDYIQQLSTQDPLKMEETLESILMDAFSSHELVILDDLHLIYDVICGCG
                     YGSAYPRGKYVEAILTSIAGRIIESGKKLILSFEGSIPAPIRSRCYISSIQKFKAADY
                     STICSNILEEKAASLDFQKIHRFAPKLNAHQLNTSALWLKQEEVDTDRFIEYLRSQRM
                     GSNVDLEEVEAIDLYSLRGIDDVIQSLEANIIVPLERDDLATEYGISPKRGVLLAGPP
                     GTGKTTVGRALAHRLRSKFFLIDGTFISGSRDFYQKVQHVFETAKHNAPSIIFIDDSD
                     VIFENKEDFGLYRYLLTMLDGLESESNKRVCIMMTTMDVASVPPALVRSGRIELWLET
                     RLPDNEARLKILIDQAHRLPPELRSYDPLRLAEITDGLTGADLKRLIEDAKILLAYDK
                     SRNVECEDLFSYFERATETIRSNKLKYEAAEERSKQRAPSMKEALEAAFSSMGGYGFD
                     TDNFPD"
     gene            4820..>5229
                     /locus_tag="PKW73_01985"
     CDS             4820..>5229
                     /locus_tag="PKW73_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72079.1"
                     /translation="MEVSSIRRIRGALTVTVVLSFAFCGVAGLAPGAIAQNSCQQLQA
                     SMRERGQRLGMANGISLRAIGSIEEHKAREALTLSNQALAYTTNSPGPYISRGLAELM
                     LNDAAAAYNDFRQSLALTEADIAAVKKLEAMNVPG"
BASE COUNT         1091 a         1322 c         1403 g         1413 t
ORIGIN      
        1 tatggtcgga ccttcccggc aggttggcca tcgactgaga gatttgatga aagatggtgc
       61 cgccgcgctc tcttcttttg tggattgcgg cagcaagacc gtggtcatcg ttggtggcgg
      121 catggccggt ctttcggccg gatggtggct gaagaaaaac ggcattgagg acttcctgat
      181 tctggaactt gagccccagg tgggtggtaa ttccagtttt ggcgaaaatg cagtcagtaa
      241 attcccctgg ggggcgcact atgttcccct ggctaacagt gagtccaaat acgtgcggca
      301 gctctttctg gaactgggca taattgaagc cgatcatgat aaggaaaagc ccaggtacaa
      361 cgaactttat ctctgccacg atccgcaaga gcggcttttc aaagacggca gctttcagga
      421 aggtctggtg cccaagcggg gtcttaagcc atctgaggaa gcggagatga agcgcttctt
      481 tgacacggtg catgagctaa gggatgtaaa aggcagcgac ggcaagccgg cttttgctat
      541 tcctcttgat catagttctg aggacccgcg ctttcgggac ctggataaga tctcatttgc
      601 tcgctggctc caggagaata atttcgtcag ccgccccctg aactggtatg ttaattactg
      661 ctgccgtgac gattacggtg ccactgccga gaatgtctcg gcctgggccg gtctgcatta
      721 tttcgccggc cgcaggggcc atgccgctaa tgccgagacc aattcagtag tcacctggcc
      781 tgagggcaat ggttttctag tttcgcgcct caaggaactg cttgccgaca agatagttac
      841 aaacgctccc gtgctgcgtc tcactgaaga taaggagggt ggtagcctct gttacttcac
      901 tagagacggc gctgtttcaa gatacaaaac taagttcacc atcttttgcg cccctcgctt
      961 tctggcaccg tatatggtgg aaaagtacaa acttgagaag aagcccactt acgctccctg
     1021 gctggtagcc aacattacct tgaagaaggc gcccgccgga cgcggtgtcg gcttagcctg
     1081 ggacaatgtc agctattacg gcgattccct cggctatgta gtggccaacc atcagaatat
     1141 caccacacgc cggagcagcg ttgttgttac ctattacctg ccgctttccc agttgccgcc
     1201 agatgcggcc aggcgcaaac ttttgaatag cccggagaag gcctggtcgg ccgccatagt
     1261 agacgatctg gaagctatgc atccgggcat taccgaggac atcatttcta tagacctctg
     1321 gccctggggg catggcatga tttcaccggg tgtagactat atctggcagg aaaggcgcaa
     1381 actgcccctc aatcagggag cggtgcactt tgcccattcc gacatgagtg gtatttccaa
     1441 tttcgaagag gcacagtacc agggtgtgca ggccgccgag agggttctta cccaactgga
     1501 gaagagttga aatggagagc acccattctc tacctctgga aaggaagcag cggcagccct
     1561 ggctgtcctc gcctggcttc gatctgctct tcattctgtc gccggccctc atttcttcca
     1621 cttttgccct gctctttggc cggcgcttcg agggtatgga aaacttgccc ctctttgcct
     1681 gggttgtgct ggtgcttatg gtggatgtgg cccatgtcta tgccaccctc tttcgcacct
     1741 atttagataa agaggcactt gccgccaata agcgtttgct cctggtcata cctcttgcct
     1801 gccaggtggt aggggttctg ctctacagcc tcgatgctct ctatttctgg agggctctgg
     1861 cctatctggc ggtcttccat tttgtgcgcc agcagtacgg ctttcttgct ttgtatgggc
     1921 ggggtgatgc gcaagtgcct acctgggcgc ggcatttgga tcagacttgt atttacacgg
     1981 taacgctcta tccgatttta tactggcatg cccacgcgcc ccgccacttc aactggtttg
     2041 tggaaggaga cttcttctcg gcctcggctc tggcatctgc cattttgccc ctggccggtg
     2101 ttttctatat attggtcgct atcgcttatt tcatcaaaga gtgcctcctc tttaagaaca
     2161 acggctttgt caatctgccc cgcaacttgc taatgcttgg aacagcgctt tcctggtatg
     2221 tgggtattgt tgccaccaat tccgatttga ttttcaccat gaccaatgtc ttgagccacg
     2281 gtatacccta tatggctttg atctggctgt atcgcaaggg tggtggcgcc ggtagtgcgg
     2341 atgccggtgc tgctggaagc ggagttttgc aagtcctggt ttcttttgcg ccggcatttt
     2401 ttgccttcct ggttttcctg gcttacctgg aagaaggttt ctgggacggc tttgtctggc
     2461 gggagcatct cactttcttt gcgccttttg ccgggctgcc ggtcatatcc gacccggcct
     2521 ttctggccgt tctggtgccc cttctttctt tgccccagag cacccattat gtgctggatg
     2581 gttatatctg gcgggtgaag gaccgaaaca gccagtggtc ggcttgagcc tgctagtgga
     2641 actatgtctt tcttaaagcc tgtgccaggc acaaagcagc cccctccgag ttgatcggag
     2701 cgggctgcca aatgttcaag tgcgtgagat tggagctaac tattcactga tattccactc
     2761 tcattccagg tcggattctt attgcattct tgagggctgc aacttgcaag gaacagcaaa
     2821 tccaaaccat gtctcacttg agcgtactgc cgagattcgg cgcacatcag cctgacagag
     2881 tgcttgattg aataagggcg aaccattcat agaatggggc gctaactcaa gcaagcgcgt
     2941 tgtcaagctg actctggaat agtctttgct ttgtgacagc aaaatacgat ctgccgcagt
     3001 ttatgagact gaaacaaaag ttaaaacaga gttcgcttct agagccgctt ggccttggtc
     3061 ctgagagtct gaggatcagt ctgggaagtt gtcagtgtca aatccatagc cgcccatgga
     3121 actaaaagct gcttccagag cctctttcat tgacggcgct cgctgttttg aacgttcttc
     3181 cgcagcttca tatttgagct tatttgaacg aatggtttca gtggcccgct cgaagtatga
     3241 gaacagatct tcgcattcga catttctgga tttatcgtaa gccaggagaa tcttcgcatc
     3301 ttcaatcagt ctcttcagat cggcgccggt aagcccatct gttatctccg ccagtctgag
     3361 agggtcgtaa ctgcgtagct ccggcggcaa ccggtgtgct tgatcgatga ggattttcag
     3421 cctggcttca ttgtcgggca gtcttgtttc aagccatagt tcgatacgcc ccgaccgcac
     3481 aagggccggc ggcacgctgg ccacatccat tgtggtcatc atgatgcaga cccgtttgtt
     3541 gctctcactc tcgagaccgt caagcatggt cagtaagtag cggtagagtc cgaaatcttc
     3601 cttgttttca aagattacat cactgtcgtc gatgaagatt attgagggcg cgttgtgctt
     3661 ggccgtctcg aaaacatgtt gcactttctg gtagaaatcg cgactgccgg aaatgaaggt
     3721 accgtctatg agaaaaaact tgctgcgcag cctgtgggcc agtgcccgac ctaccgtggt
     3781 cttgccggtg ccgggcggtc ctgccagaag cactcctcgt ttggggctga tgccatattc
     3841 ggtagccaga tcatctcgtt ctaaaggaac gattatgttg gcttccaggc tttgaatgac
     3901 gtcgtcgatg ccgcgcaagg aatagaggtc tatggcttct acttcttcca gatccacatt
     3961 gctgcccatg cgctggctgc gcaggtattc gatgaatcta tccgtatcca cttcctcctg
     4021 tttgagccag agcgctgatg tattgagctg atgagcattc aatttggggg cgaagcgatg
     4081 aatcttttga aagtccagag aggctgcctt ttcttccaga atgttgctgc aaatagtgct
     4141 gtagtctgct gctttgaatt tttgaatgga gcttatgtag cagcggctgc gaatgggagc
     4201 cggtatgctt ccctcgaaag aaagaattag tttctttccg gattcgatta tgcggccggc
     4261 tatgctggtc aaaattgctt ccacatactt gccgcgggga taggcgctgc catagccgca
     4321 tccgcaaatg acgtcatata tgagatgcaa gtcgtccaga attaccagct catgagagct
     4381 gaaggcatcc atgaggatac tttctaaggt ctcttccatt ttcagcgggt cttgtgtact
     4441 cagttgctga atgtaatcgg tcattgtcaa aaatttgccg cctttgagcc cgtgcaattc
     4501 tttgagaatg gtggtcttgc ccatgcctgt atgggaccag actacaaaga tgtttcctat
     4561 gggcagagct ttaataagct cgtcgaaggc agctgtctgg gccggggata aatcagtcat
     4621 gttacacctc agcatctaag ctagttgtca tagcattttc ttccccggtt caaggcttac
     4681 tgtttggttt tcactattaa aatttccggt ttccggggct tgtttccaaa agcttgtgtg
     4741 aacaagcttc ttgagaattt attgggaggc tggggcgact ataattgtgc tcgggtttct
     4801 aattcaagat tggatggtta tggaagtgag ttctatcagg cggataaggg gcgctctcac
     4861 tgttacagtg gtgctctcat tcgctttctg cggtgttgcc gggcttgcgc ccggggcaat
     4921 agctcagaat agctgccaac aattgcaggc ttccatgcgg gagagggggc agcgtcttgg
     4981 catggctaat ggcatttccc tgcgggctat tggttcaatc gaagagcaca aagccaggga
     5041 agcactgact ctcagtaatc aagctcttgc ttatactacg aattctcccg gcccttatat
     5101 cagccgcggc ctggctgaac tgatgctgaa cgatgccgcc gccgcctaca acgatttcag
     5161 acagagcctc gctctcaccg aggctgatat cgccgccgtc aagaaactgg aggctatgaa
     5221 tgtgccggg
//
LOCUS       DAOQNZ010000067         5216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6507_length_5216_cov_4.073435, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000067 DAOQNZ010000000
VERSION     DAOQNZ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6507_length_5216_cov_4.073435"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKW73_01990"
     CDS             <1..647
                     /locus_tag="PKW73_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA72080.1"
                     /translation="GQDYVDLYQCHRFDPETPMEELVRTMDDLTRQGKILYWGVSEWS
                     GSQIEDAYRVAAELNAVPPISNQPCYNMLTRNIEASVIPVCERLGVGQVVFSPLAQGV
                     LTGKYAPGKPLPEGSRAADSRVNMFMMGRSLMSDETLMKVERLKAIAQDEGLTLGQMA
                     LAWCLRETNVASVIIGATKPQQIEENVTAAGLKLSSEALAKIETILATPVGARA"
     gene            747..2207
                     /locus_tag="PKW73_01995"
     CDS             747..2207
                     /locus_tag="PKW73_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72081.1"
                     /translation="MKLKKGGACLSALLLLASSLPVAQVFLALPCLADDPQVQSDWAV
                     DDPNRKPAVTEEKPKSEESGGTSLVAPVVPPAAGESAKSDSPASSTDALDMPMVAPVM
                     SPATQAVINSNAKQTIEKGAVTATATVKPAAPSQTIQASASSAKLYGRIEQIAGGKGA
                     NFPIVFKAAVPSLDTSLAKPALKGGAQDLTVYSGTVAKSFPEDYRGTWGGSLKVWTVE
                     QAPVCFQIDPEEAGKVQRIFKSGAEGSVNFMFANDTKGGIYLAPAQIMFQVAGKEVGL
                     EKQMTQMMGGQSLSAMGPMGQMMAQMAQSMPVPIIFSFGDIQTNSMAKGLSGNEFVQR
                     TLRNTVRQLTPQVIEQQIIAQMNEVMKSTGRPRLRYEESVIRFTKVNAQQMYVQAAQV
                     VYGPDRKFQQKIIMYGYVTKGQVVNTNPYSGMMQMPGMMQIPGQAPGTMPQMRPGTIP
                     SIPGLTPGQNPFGGGNGGSIPGLPPGFNPFQGLFGQ"
     gene            2224..2802
                     /locus_tag="PKW73_02000"
     CDS             2224..2802
                     /locus_tag="PKW73_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNA72082.1"
                     /translation="MSTTYSSELLSELAQFVSDLISQSDYPGFESRAFRIGLPDRFNQ
                     ASSKLFGECLDHESVYMRLVALRWFQDKPGTIKPFIKQIQAHLLDGDEWVRIEALGAL
                     ERFHSPTSGNAQACALLLKDENVQVRKASAKALTKMLPRVHEKLAKELDDVRQENLKQ
                     VETLLTEALCDSDVQVRQKAEKALRKSGNLDG"
     gene            complement(2807..3322)
                     /locus_tag="PKW73_02005"
     CDS             complement(2807..3322)
                     /locus_tag="PKW73_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72083.1"
                     /translation="MTLHNIKEEEIVKQIVSCLDANQALTVKIHEVIERFSEKRVTLE
                     EKMIRSYFHEMAAVLFVINATVHRQMSQIYTSAGKQNARKEPSALRQDAGRIKEIEEG
                     LTALAAALEDLRGLQEKISVQLADLLKVLAYNLNFMEQYFEYGFYTRINNDDNCLEAL
                     KLLENRLAAES"
     gene            complement(3423..3656)
                     /locus_tag="PKW73_02010"
     CDS             complement(3423..3656)
                     /locus_tag="PKW73_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNA72084.1"
                     /translation="MPKKVLIALPPAMLEQVDFIAQCEHRTRSDLIREALRRYLDNFR
                     RQQGGTHLSVSTMEMSDSFQAAPVAGSTSGSST"
     gene            4445..>5216
                     /gene="xth"
                     /locus_tag="PKW73_02015"
     CDS             4445..>5216
                     /gene="xth"
                     /locus_tag="PKW73_02015"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNA72085.1"
                     /translation="MLIASWNVNSIAVRLPHVLNYLKESKVDHLGLQELKTTDDKFPY
                     TEFSEAGYSCLIKGEKTYNGVALVSQKETPCRLVADVLPGAPEPLQSRLIDVLLPQSK
                     LRILNLYVPNGSQVDSDKYEYKLKWFDALLTYVGKAQESGEHLLIMGDFNIAPEDKDV
                     HDPAQWEGQVLVSERERQCFQRLLSLGFTDSFRHLHEEGGVFSWWDYRQMAFRRNMGL
                     RIDHILLSQSLLPFLKQAWVDKSPRKLEKPSDHAPVLVE"
BASE COUNT         1224 a         1273 c         1412 g         1307 t
ORIGIN      
        1 tggggcagga ttatgtagat ctctatcagt gccatcgctt tgatccggag acgcccatgg
       61 aagaactggt tcgaaccatg gatgatttga cccggcaggg caagattctc tactggggag
      121 tgagtgagtg gagcggctct caaatagagg atgcctaccg ggtggcggca gaactcaatg
      181 ctgtgcctcc catttccaac cagccctgct acaacatgct gaccagaaac atcgaagctt
      241 cagttatccc tgtttgcgaa aggctgggag tcggtcaagt tgtgttctcg cccctggctc
      301 agggtgtctt gaccggtaaa tatgcgccgg gtaaacccct gccggagggc agcagagctg
      361 ccgacagtcg ggtcaatatg tttatgatgg gcagaagtct gatgtcggac gaaaccctga
      421 tgaaagtgga aagactcaag gctattgccc aggatgaagg cctgactctc gggcaaatgg
      481 ctctggcctg gtgcctgcgt gagaccaatg tagccagtgt cattatcggc gctaccaaac
      541 cccagcagat agaagaaaac gtcacggctg ccggtcttaa gctttccagc gaagctctgg
      601 caaaaataga gacgatcctg gctacccctg tgggtgctag agcctgagac agaagcgacc
      661 ttattgttac tctttcttgg cccggtgggt agaccggccc ggaagcgggc acaactaatg
      721 atgcagctac tattcagggg gtcggtatga aactgaaaaa gggtggggct tgcctttcgg
      781 cgctcttgct attggcttca agcctacctg tggctcaggt cttcctggcc ttgccctgtc
      841 tggctgatga cccgcaagtg cagagcgact gggcggtgga cgaccccaat cgcaagcctg
      901 ccgtcactga agagaagccc aagagcgaag agagcggcgg cacttcctta gtggccccgg
      961 tcgtccctcc tgcagccggt gaaagcgcta agtccgatag ccctgcctcc tccactgatg
     1021 ccctcgatat gccgatggtg gcgccggtga tgagcccggc cacccaggcc gtgatcaaca
     1081 gcaatgccaa gcaaaccatt gaaaaaggtg cggtgacggc cactgctacg gtcaaaccgg
     1141 cggctcccag ccagaccatt caagcttcgg cttcctctgc caaactttat ggacgtattg
     1201 aacaaattgc cggcggcaag ggtgctaact tcccgattgt cttcaaggcc gccgtacctt
     1261 cccttgatac ctctctcgcc aaaccggcct tgaagggcgg tgcccaggat cttacggttt
     1321 actcgggcac tgtggccaag agtttccccg aggattatcg cggcacctgg ggcggctctt
     1381 taaaggtctg gactgttgag caagctccgg tttgcttcca gattgatccg gaggaagccg
     1441 gcaaggtgca gcgcatcttc aagagtggtg ccgaaggttc cgttaatttc atgttcgcca
     1501 acgatacaaa gggcggaatc tatctggctc ctgcccagat tatgtttcaa gtagccggta
     1561 aagaagttgg ccttgaaaaa cagatgactc agatgatggg cggacagagt ctttcggcca
     1621 tgggaccgat ggggcagatg atggctcaga tggcacagtc tatgccggtt ccaattatct
     1681 tttctttcgg agacattcag accaattcca tggccaaggg gctctccggt aatgaatttg
     1741 tgcagcgtac cttgcgcaat acggtgcggc aattgactcc ccaggtaatt gagcagcaga
     1801 tcatcgctca gatgaatgaa gtaatgaaat ctaccggcag accgcgcctt cgctatgaag
     1861 agtcagtaat tcgctttacc aaggtgaatg ctcagcagat gtatgtgcaa gcggctcagg
     1921 ttgtctatgg tcctgaccgc aagtttcagc agaaaattat catgtatggt tacgtgacaa
     1981 aagggcaggt ggtcaatacc aatccctact ctggtatgat gcagatgccc ggcatgatgc
     2041 aaattccggg acaagctccc ggtactatgc cgcagatgcg tcccggtacc attcccagca
     2101 taccggggct gacgcctgga caaaatccat tcggcggcgg taatggtggg tccataccag
     2161 gactgccacc aggtttcaat cccttccagg gactttttgg tcagtaagcc ttagaaaata
     2221 aacatgagca ccacctattc ctcagagctt ttaagtgagc ttgcccagtt cgtttccgat
     2281 ttgatctccc agagtgatta tcccggtttt gagtctcgcg cctttcgcat cggtttgccc
     2341 gatcgcttca accaggcctc gagcaaactc tttggtgagt gcctcgacca cgagtcagtc
     2401 tatatgcgac tggtggctct gcgctggttt caggacaagc ccggcaccat caagcctttt
     2461 atcaaacaga ttcaagctca tttgctcgat ggggacgaat gggtgcgtat agaagccctg
     2521 ggtgccctgg agcgcttcca ttctcccacc agcggcaacg cgcaagcgtg cgcgcttctg
     2581 ctcaaggacg agaacgtaca ggtgcgaaag gccagtgcca aagcgctcac taaaatgttg
     2641 ccgcgtgtac atgaaaagct ggctaaagag cttgacgacg tgcgccagga aaatctcaag
     2701 caggtggaaa cgctcttgac ggaagccctc tgcgacagcg acgtgcaggt gaggcagaaa
     2761 gcagagaagg cactgcgcaa gagcggcaat ctggacggct agtttttcaa ctttcggcgg
     2821 cgagacgatt ttctagaagc ttcaaggctt ccaggcagtt gtcgtcgtta ttgattctgg
     2881 tatagaagcc gtactcaaaa tactgctcca tgaaattcaa gttgtaggca aggactttca
     2941 gcaaatctgc cagttgaacc gagatcttct cttgtaagcc tctgaggtcc tctagagcag
     3001 cggcaagtgc ggttagcccc tcttctatct cctttattct tccggcgtct tgccggaggg
     3061 cagagggctc tttcctggcg ttttgttttc ctgcgcttgt atagatttga gacatctgtc
     3121 tgtgcactgt tgcatttatg acaaagagta cggcagccat ctcatggaaa tagctgcgga
     3181 tcatcttttc ttccagagtg acgcgctttt ccgagaagcg ttcgattacc tcgtggatct
     3241 tgactgtgag cgcttgattg gcgtcaaggc aagagacaat ctgcttgacg atctcttctt
     3301 ctttgatgtt gtgtaaagtc ataatccctg agccttttaa ttgccttcct acaaagaaaa
     3361 acagccgccc ggctgggcga ctgttttctt aaggttggtt ttcgtttccg ctttgccgag
     3421 tcttatgtgg aagaaccgct ggtgctgccg gctacgggtg cggcctggaa ggaatcggac
     3481 atttccatgg tgctgacgga aaggtgagtg cctccctgct gtcttctgaa gttgtccaga
     3541 tatctacgca gagcttcgcg tatcagatct gagcgggtgc ggtgctcgca ctgggcaata
     3601 aaatcgactt gttcgagcat agcagggggt agtgcaatca agaccttctt gggcatattc
     3661 gattcctcat ggagacgttt tggggtgacg aaattcgcct ctttcaaagc cacacgcttt
     3721 gtgagaggga actttatcaa cggttcttta cgataggtaa ccgctttttg ggtggcgagt
     3781 ggatttatca accaccttca acggctattc aatttacaca tgaaagtcga tatcgtaaag
     3841 gtctaaaagg ggcataaata cccaagttcc tagaaataca cttaaaaggt tttttggaaa
     3901 gtcgcaatcc ttgaccaggt aagactcaag ggggatcagc ctaaaatccg tatagatact
     3961 agggtggaca aaggacatat cctgctcttg aacttctaaa aaattgtgct agagattccg
     4021 attgtttggc gcattttgcc cggtgcttct gatgaaagtg gcttgtgcag ccagttgtaa
     4081 tacgatattt agcgttttat ctggtacaga ggtgacaggc ttttgatcgg cgaaataagc
     4141 gatatgcatg ttatgtccta gacgtaagcc ggacggcatc tacttatcat taaatagtat
     4201 cagtggtgat accactaccg tatatgcgtt tttcgagacg ggcgtaatag ccctttcttc
     4261 agctcttaaa acggcatggc ctgcgcgttt ggagccagag attgggcaga ctgcccagac
     4321 gtgtgagttt tctacaggat ttgaactgtc agggtgtttg ctcttggttt taaaaagtca
     4381 aggcttggta aaacggtacg atacattgca tcattgatat ggttgtcgga agagggaaaa
     4441 agtgatgctc atcgccagtt ggaatgtcaa ctccattgcc gttcgtttgc cccatgtttt
     4501 gaattatttg aaggagtcaa aggtagacca tctgggcttg caggagttaa agactaccga
     4561 tgataaattt ccgtacaccg aattttcaga ggccggctat agttgtctaa tcaagggtga
     4621 gaaaacctat aacggtgtgg ccctggtttc tcaaaaagag actccctgcc gcttggtggc
     4681 agatgtcttg cctggtgccc cggaaccgtt gcaaagtcgg cttattgacg tgcttttacc
     4741 gcagtcgaaa ctgcgcattc tcaatctcta tgtgccgaac ggctcccagg ttgattccga
     4801 taagtatgaa tacaagctca aatggttcga tgcgcttttg acctatgtag gtaaagcgca
     4861 agaaagtggt gagcacttgc tcattatggg tgatttcaat atcgcccctg aagacaagga
     4921 tgtgcacgac ccggcccaat gggaagggca ggttttagtt tcggagcggg aaaggcagtg
     4981 ttttcaacgc cttctgtctc tgggcttcac agacagcttc aggcacctgc acgaggaggg
     5041 aggagtgttt tcctggtggg attacaggca gatggctttt cgccgcaata tggggctgag
     5101 aatcgaccac atattacttt ctcagtcgct gcttcccttt ctcaagcagg cctgggtcga
     5161 caaaagcccg cgcaaattag agaagccctc tgatcacgct ccggtactgg tggaac
//
LOCUS       DAOQNZ010000068         5213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6510_length_5213_cov_3.088212, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000068 DAOQNZ010000000
VERSION     DAOQNZ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6510_length_5213_cov_3.088212"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PKW73_02020"
     CDS             <1..792
                     /locus_tag="PKW73_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNA72086.1"
                     /translation="AGYAEVMETVFAAWQHIPINENHIKQLHRDLLRHSSKDDRHRGE
                     YKKLSNSVAAFDEHGKQIGIVFETATPFDTPHLMAELVAWLTETRDQHRLHPLLIAAV
                     FIVVFLEIHPFQDGNGRLSRILTTLLLLQAGYAYVPYSSLESVIENNKEAYYLALRQT
                     QGTIRTDSPNWQPWLAFFMRALQQQTRRLAVKVEREKFVLADLPELAVKILDYTRDQG
                     RVTMRDMVRATGASSNTLKEHFRNLVKKKLLERHGAGRATWYTLP"
     gene            complement(832..1629)
                     /locus_tag="PKW73_02025"
     CDS             complement(832..1629)
                     /locus_tag="PKW73_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA72087.1"
                     /translation="MAFEDWKKTEGEKQTATAVESNSGLSWVSEAALGAYRDARSAMP
                     VEEPYKDLALAGTAVLGLASATLVARKALVSESLQIVGKEVSIVPLTKENLPKAVAAA
                     NDGFRYGWPILHPARDFKASLNPAVNAKRLAHVSDDFKIELNARYWVAKDRNGNVFGT
                     TGLYETGRDQSEAAWLGWMSVRNNARGLGIGRKLVEFSEEQARADGKQFLRLYTSTSK
                     GEAAAQVLYEKLGYKIVERTPHSLPRFIQRLAGEKQPLEILLREKRL"
     gene            complement(1688..4021)
                     /locus_tag="PKW73_02030"
     CDS             complement(1688..4021)
                     /locus_tag="PKW73_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72088.1"
                     /translation="MSELAGSEGQNVKPDMSSGDWLTAELKPALKPEPAQLDLHPTSL
                     RMSAALPGLNLSGDKAADNQTTPALAAKNLVVDASTDAASKDVKEVGLARWTESNVVN
                     PAANVGADAYNAVVYNTVGKLANSASQVIADKDIMKRAEHREVGEVTGMSKEMVSQAV
                     VSGVGMAIVYVAAGRSMSGLIRAGSNAIGVEAKLAGRGGVENAASFSKTLLSDRTGQI
                     AGSVAYDGLRDVHAGETRLGNMAGSLTAFSIYEKFNPKLGSLNLAPRLGGYALLGAGG
                     AASSKIVADGVSGHITTGDELSRQMLAGGAMNLFIPGLQKAAGFGIGKLNDKLGRGNP
                     VERDMLLTGLAGKSQTLDELAGKVSSLRVKTGQETTNLQGKEVSLAAKGDGSANNSAA
                     DRAHEFAHEWFLKSNKEEFAGAARLLKEGDEAGAFARYKQLRLESETYAHKMEAKVAS
                     EAGETRVVETNPEKIAALKTPEGISYEEQWKREFERFKATDGKVVAEIDYRDVPTIVA
                     VQSMREVAGQDAKAMKQIMREEYYPGLKKAFPDKSEYEKISTYYEYLLDRGGTWDAVA
                     LRGNNGQIVGGIQSQVINVEGKVIKNAVWGEHIWLSPDARSFPNFRALMNTAADRFRT
                     TGSQVAFMEFNDRAKMTLQELIQDAKGGLTTEAREVIWGRFANKGLNILHFKDGKIAP
                     YAQPGMDGQEPVTYLTLALIALDGSNLSGRKLPTNDYLKLLTKAHSTIVDPATDPTVL
                     GYTRELQNRIAAGETNMTFRRLADTTVGRIVTRQFGS"
     gene            complement(4169..>5213)
                     /locus_tag="PKW73_02035"
     CDS             complement(4169..>5213)
                     /locus_tag="PKW73_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72089.1"
                     /translation="RQLRLASETYAHKMEAKVAAEVGDRRVVEVNSEKIGALKTPAGV
                     TYEEQWRREFERFKATDGKVVAEIDYREVPTIVGVESMREVAARDPKAMKTVMKEEYY
                     PKLKQAFPDKSEYEKLSTYYGYLLEKGGTWDAIALRGSNGQVIGGIQSQVINVDGKVI
                     KNAVWGEHIWLSPDARSFPNFRGLMNVAADRFRASGSQVAFMEFNDRAKMTLQEMIQD
                     AKGGLPTEAREVIWGRFANKGLNVLHFKDGKIAPYAQPAMDGQAPVNYLTLALIALDG
                     SNLAGRKLPTADYLKLLQKAHGTLVDVNTDPTVQAYTRELQTRIDKGDTRMTFTRLAD
                     TTVGRIVTRQFGS"
BASE COUNT         1147 a         1519 c         1273 g         1274 t
ORIGIN      
        1 gctggctatg ccgaagtcat ggaaaccgtc tttgccgcct ggcaacacat tccaatcaac
       61 gaaaaccaca tcaaacaact tcaccgcgac cttctacgcc acagtagcaa ggacgatcgc
      121 caccgtggcg aatacaaaaa actgtccaac agcgttgccg ctttcgatga gcacggcaaa
      181 cagattggca ttgttttcga gacggcgacg cctttcgaca caccgcacct catggctgag
      241 ctggtcgcct ggcttacaga aacccgcgat cagcaccggt tgcatccctt gctcattgcc
      301 gccgtgttca ttgtcgtgtt cttggaaatt caccccttcc aggacggcaa cgggcgctta
      361 agccgtattc taaccacctt gcttctactg caagccgggt atgcctacgt gccgtatagt
      421 tcacttgaga gtgttatcga gaacaacaaa gaggcttact atctggcatt gcggcagaca
      481 caaggcacca ttcgcacgga ctcaccaaac tggcagccct ggctggcatt tttcatgcgc
      541 gccttacagc agcaaacgcg gcgactggcc gttaaggtcg agcgcgaaaa attcgtactg
      601 gccgacttac cggaattggc agtcaaaatt ctagactaca cccgcgatca aggccgcgta
      661 actatgcgcg acatggtgcg cgcaactggt gcaagcagca acactctcaa agagcatttt
      721 cgcaacctgg tcaagaaaaa gctattggaa cgccacggcg ccggacgcgc cacctggtac
      781 acgctgccct gatcatccaa caacaaacaa ttgcggcagc gccactatca cctacagcct
      841 cttctctcgc agaagaatct ccaggggttg tttctctccc gccaggcgct gaatgaagcg
      901 cggcagactg tggggtgtgc gctctacaat cttgtaaccc agcttttcat agagcacctg
      961 ggcagcggcc tcgcctttgg aggtgctggt ataaagtctg agaaactgct tgccgtcagc
     1021 cctggcctgc tcttctgaaa actccaccag ctttcttcct attcccagac cgcgagcatt
     1081 gttgcgcacc gacatccagc caagccaggc ggcctctgat tgatcgcgac cagtttcata
     1141 aagaccggtg gtaccaaaga cattgccgtt cctgtcttta gccacccagt agcgagcgtt
     1201 caactcaatt ttgaaatcgt cagacacatg cgccagccgc ttagcattaa ccgccgggtt
     1261 caaggatgct ttgaaatctc tggccggatg cagaatcggc catccatagc gaaagccgtc
     1321 gttggctgcc gccacagctt tcggcaagtt ctctttggtg agcggcacaa tcgaaacttc
     1381 tttgcccaca atttgaagac tctccgatac caaagcttta cgcgccacta acgtcgccga
     1441 agccagaccc aacacagccg ttccggccag ggccagatct ttatagggct cttccaccgg
     1501 catggccgag cgagcatcac ggtaggcacc gagagcggct tcgcttaccc agctcagacc
     1561 ggaattagat tcaacagccg tagcggtttg cttttcacct tcagttttct tccagtcttc
     1621 aaacgccatg gccatccttg atgcttcttc aaatgccgca ctaactgcct cttgtaacag
     1681 acaccgccta agaaccaaac tgcctggtga cgatgcgacc cacggtggtg tcggccagac
     1741 gcctgaaggt catattggtt tcgccggcag cgatgcggtt ttgcaattct ctcgtataac
     1801 cgagcacggt aggatcagtt gccggatcaa caatagtgct atgagctttg gtgagcaatt
     1861 tcaggtaatc attggtgggc aattttctac cggacaggtt ggaaccatca agagcaatga
     1921 gagccagcgt caaatatgtc accggttcct gtccatccat gccgggctgc gcataagggg
     1981 caattttgcc gtctttgaag tgcaagatat tcaggccttt gttggcaaag cgaccccaga
     2041 tcacttccct ggcttccgtg gtcaagccgc ctttagcgtc ttgaatcagt tcctgcaaag
     2101 tcattttggc tctgtcgttg aactccatga aagccacctg agagcctgtg gtcctgaatc
     2161 tatcagcggc agtgttcatc aaggctctga agttggggaa agaacgagcg tcgggactga
     2221 gccatatgtg ctctccccag acggcgtttt taattacctt gccctctacg ttaatgacct
     2281 gcgactgaat gccgcccact atctgtccgt tgttgccgcg cagggcaacg gcatcccagg
     2341 tgccgcccct gtccagaagg tactcgtaat aggtagaaat cttttcatac tcagacttat
     2401 cagggaaagc ctttttcaag ccgggataat actcttccct cattatttgc ttcatggctt
     2461 tggcatcttg cccggctact tcccgcatgc tctgcacagc aactatggtg ggtacatcgc
     2521 ggtaatctat ttcagccacc actttgccgt cggtagcctt gaagcgctcg aattcacgct
     2581 tccactgttc ttcgtagctt atgccttccg gtgtttttag ggcggcaatc ttttcaggat
     2641 ttgtctcaac gactctcgtt tcaccagctt ccgaggctac tttagcctcc attttgtgcg
     2701 cataggtttc agactccaga cgcaattgct tgtaccgggc aaaagcaccg gcttcatcgc
     2761 cttctttcaa gagccgggca gcaccggcaa attcttcctt attcgacttc aagaaccatt
     2821 catgagcaaa ttcatgggcc cggtctgcgg cggaattatt ggcgctaccg tcgcctttgg
     2881 ctgccagact gacttccttg ccttgcaaat tagtggtttc ttgcccggtc ttcacccgca
     2941 gggaagaaac cttgccggcc agttcatcca gggtctgaga cttgcctgcc agaccggtga
     3001 ggagcatgtc gcgttcgacg ggattacccc ggccaagctt gtcgttaagt tttccgattc
     3061 caaaaccggc tgctttctga aggcccggaa tgaaaaggtt catggcgccg ccggccaaca
     3121 tctgacggct cagctcatca ccggtggtga tatgacctga gacaccgtcc gccacaattt
     3181 ttgaggaagc agcaccgccg gcaccaagaa gcgcatagcc gcccagtctg ggagccaggt
     3241 taagagagcc cagcttggga ttgaattttt cgtaaatgga aaaagcagtg agcgatccgg
     3301 ccatattgcc gaggcgagtt tcgcccgcat gcacatcacg caatccgtca taggcgacag
     3361 aaccggcaat ctgtccggtt ctatcgctca agagggtctt actgaaagag gcagcattct
     3421 caacgccgcc gcgaccggcc agttttgctt ccacgcctat ggcattggaa ccggctcgaa
     3481 tcaagccact catggagcgt cccgcggcca cataaacaat ggccatgccc accccgctca
     3541 ctacagcctg actgaccatc tccttgctca tgccggtcac ttcaccgacc tcacggtgct
     3601 cggcgcgttt catgatgtct ttgtcggcaa tcacctgaga agcggaattg gctaacttac
     3661 caacggtgtt atagacaacg gcattgtagg catcggcacc cacgttagcg gccggattca
     3721 ctacgttcga ctcggtccac ctggccaatc cgacttcttt cacatcttta ctagcggcat
     3781 cggtgcttgc atcaacaact aagttttttg cagccagggc cggtgtagtt tgattgtcag
     3841 cggctttatc accggataag ttgagaccgg gcaatgctgc agacattctc aggctggtgg
     3901 ggtgtaaatc caactgcgca ggctcaggct tgagcgccgg tttcagttcg gcagtcaacc
     3961 aatctccact actcatgtcc ggctttacgt tctgaccttc cgatccagct agttctgaca
     4021 tacactacaa gcctcagggg attttgccgg caccggcaga ctgctcctgc cgtctttcaa
     4081 agctaacgtc cctctgtgtc caaagatggg caaactcggt ggtgagaata cgaaagccgc
     4141 attaaacggc gtcttaccca aacttgattc aggaaccaaa ttgtcttgtg acgatgcggc
     4201 ccacagtggt gtcagccaga cgagtgaagg tcattcttgt gtcgccctta tcgattcttg
     4261 tctgcagttc tcttgtgtaa gcctgcacgg tgggatcggt gttcacatct accagagtac
     4321 cgtgggcttt ctgaagcagc ttcaggtaat cggccgtggg cagttttctg cctgccagat
     4381 tggagccgtc cagggcaatg agagccagag tcaggtagtt gaccggagcc tgaccgtcca
     4441 tggccggctg cgcatagggg gcaatcttgc cgtctttgaa gtgcagcaca ttcaaacctt
     4501 tgttggcaaa acgaccccag attacttctc ttgcttccgt gggcaagccc cctttggcgt
     4561 cctgaatcat ttcttgcagg gtcatcttag ctctatcatt gaactccatg aaagccacct
     4621 gagagcctga tgccctgaat ctgtcagcgg ccacattcat caagcccctg aagttgggga
     4681 aagaacgagc atcggggctg agccagatgt gttctcccca gacggcattt tttataacct
     4741 tgccgtctac attaatcacc tgagattgaa tgccgccaat tacctgacca ttgctaccgc
     4801 gcagggcaat ggcgtcccag gtgccgcctt tctcaagcag gtagccgtaa tatgtagaga
     4861 gtttctcgta ctcagactta tcaggaaagg cttgcttgag cttaggataa tactcttcct
     4921 tcattacggt cttcatggct ttcgggtctc tggcagccac ttcccgcatg ctctctaccc
     4981 caacaatagt cggcacttcc cggtagtcta tttccgccac cactttaccg tcggtagctt
     5041 tgaagcgctc gaattcccgc cgccactgct cttcgtaagt tacgccggct ggggttttga
     5101 gggctcctat cttttcggaa ttgacctcta ccacccttct atcgcccact tccgccgcta
     5161 cttttgcttc cattttatga gcataggtct cggaagccag gcgcaactgc ctg
//
LOCUS       DAOQNZ010000069         5198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6555_length_5198_cov_3.922030, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000069 DAOQNZ010000000
VERSION     DAOQNZ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6555_length_5198_cov_3.922030"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKW73_02040"
     CDS             <1..634
                     /locus_tag="PKW73_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNA72090.1"
                     /translation="KSKIGGTAAIISEDGLMVTNKHVVEDTKGKVVDRVNLTIINAAG
                     VEEPRTAKVVKVEGKQDLALLQIEPKPGTTAEKFPALPLSKVTSWREREPLVEMGNAN
                     GEGKISMAKARYADMVTQSDIPFDKQPPDVRQGRTMYKLESTVPHGYSGGVVLSVPGS
                     ERRDGKIHRTGTSAIRAITDYSDTNKTAYVIPAARVQELVDQYRKQQGKK"
     gene            complement(677..1138)
                     /locus_tag="PKW73_02045"
     CDS             complement(677..1138)
                     /locus_tag="PKW73_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72091.1"
                     /translation="MGYYSNEIEQVKPPDTFFDGNKVLQEDELARRLRSNDSTDRQSQ
                     VDPSKPEPETKPEQNRASDQPPGSGAKIRSYDDFIEKLVEDSMADTHRRNLRDDERRF
                     QREREEEMRITAMAAQRELEYKLQAERMERLQSKQEHSTTNSKSWSKYLPW"
     gene            complement(1203..1874)
                     /locus_tag="PKW73_02050"
     CDS             complement(1203..1874)
                     /locus_tag="PKW73_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72092.1"
                     /translation="MTRQEKLYHLLVTFCHCAIFHPEVTSLKVTRYLLLFSLLGFACG
                     LISLANPQMTYSGSVSSDLIVLLVLLLGLGWCIRELLDLIGLYSQTSCTLCHNERPST
                     GIPWLSRYRLMEYFFLYLATCLAAGILCAGPKATDTFFLRITPAMFLPALLLLICRAS
                     GLIARIEQEIQTVLKPDLESRDLDGLSTYQLEQARELAAGIMQHHNLQLINRELNERK
                     RQLPQ"
     gene            2035..3009
                     /locus_tag="PKW73_02055"
     CDS             2035..3009
                     /locus_tag="PKW73_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72093.1"
                     /translation="MFARRLKDKTELKCEPYSESRLSLVPAPDSDPDRSQLVERACPA
                     TAEPVGRFCWLKDRLAFVSMASAAALPFAFYQFMVSYLNFSMSGVQTNEFMESTSGLS
                     CAAVCFLMVCTYEYARTLTGYKYTWFSLTWRTVALACVVFTGWTAGLSGVLTAIMALA
                     LVFAAFCLGRLIRAAVPPTFNFRRSVWNMGWCSAPGFLFLLSFVVSMPNREPSGTTYA
                     NGTAFEFFFMSAFFFLLLSIPTYAAMRAARSRETAPLALLAGLYQAPFFLATILEASL
                     VVFKPGGLTIAVSIFLCNLIAGAILLFSAGQAARANGAVHDRRISGSN"
     gene            complement(3013..3660)
                     /locus_tag="PKW73_02060"
     CDS             complement(3013..3660)
                     /locus_tag="PKW73_02060"
                     /inference="COORDINATES: protein motif:HMM:NF037025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HNA72094.1"
                     /translation="MSYEHYHRGSDNGLAQPEQNHGINSIVENTWSRADAFHFSPGKD
                     SSAAHLPGLELSGHKGDSASLSNGEQIRQFFESKGLSKEQVAGIMGNFHVEAPGFDPS
                     QRQLGGGPGFGIAQWEGSRKRDLFKYAAEHGMPADDLNTQLNFAWHELQTTHKGALKA
                     LMQTHTVEQATNVFEHKFEVAGKPNMPARVAAAQGFYGQGGTSFAWAGGTSDRLG"
     gene            complement(3747..5018)
                     /locus_tag="PKW73_02065"
     CDS             complement(3747..5018)
                     /locus_tag="PKW73_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNA72095.1"
                     /translation="MHQDNGKSLEAKLSSTLLASSVIFLILTAFIGWQWWQLSQLLSG
                     LSDLGLPAELSSNLSTLKQSNTLLGVAYILVIGTTAYTTYKIKTFVAAHVVEPLQTLT
                     SAAARVGKGELNFPIPKARDEDEIGSLTQAFDAMVIKLREDTSQITDAIDVLTNSVKQ
                     IAVSTSQSAASSSQMAATVTETTVSAEEVRQTTMVATQKGRFVADLAQQAAQISLVGK
                     NASEDTIHQMTSVREQMRAIANSMATLTDKSQAIAEITASVDELSQQSNLLAVNASIE
                     AARAGEMGKGFAVVAQEVKALSNQSKQATAEVRRILSDIQQAAAQAALSIEEGSRTVE
                     TAVRQSAEAGRSIESLAHSVAEAASAAVQIAAASQEQLTGIDQVVTAMQGIQEATKQH
                     VYSSKQIDEAAQSLTAIQDVLETLVSKYKLV"
BASE COUNT         1092 a         1288 c         1441 g         1377 t
ORIGIN      
        1 taaaagtaag atcgggggca ccgccgccat catcagtgaa gatggtctga tggtgacaaa
       61 caagcatgtg gtcgaggata cgaagggcaa ggtggtggat agggtcaatc tcaccattat
      121 caatgccgcc ggcgttgaag agccgcgcac ggcgaaagtg gtgaaagtag aaggcaaaca
      181 agacctggct cttttgcaaa ttgaacctaa gcccggcact acggctgaga aatttccagc
      241 cttgcctctc agtaaagtaa cgagctggcg cgaacgagaa cccctggtgg aaatgggaaa
      301 tgccaatggg gaaggcaaga tcagcatggc taaagccaga tatgcggaca tggtgacaca
      361 gagcgatatt ccgtttgaca agcagccacc tgatgttcgc cagggcagaa ctatgtataa
      421 actggaatcc acggtgcccc atggatactc aggcggcgtc gtgctgagtg tgccaggttc
      481 ggagcgccgg gatgggaaga ttcatagaac cggtacttca gccattcgtg ccatcaccga
      541 ttacagcgac acaaataaga cagcctacgt gattccggcc gccagggtgc aagagctggt
      601 ggatcagtat cgcaagcagc agggcaaaaa gtagctaata gtcgctggtc tgggcgtatc
      661 tgaagagttg gataaactac cagggtagat acttagacca actcttgctg tttgtggtgg
      721 agtgttcttg cttactctga aggcgttcca tcctctccgc ttgcagcttg tactctagtt
      781 ctctttgggc cgccatagcc gtaatcctca tttcttcttc tcgttccctt tgaaaacggc
      841 gctcatcatc gcgcaggttt cttctgtggg tgtcggccat ggaatcttct accagctttt
      901 caataaaatc atcatagctt ctaatctttg ctccggagcc cgggggctgg tcggaagcac
      961 gattctgctc cggcttcgtt tcaggctctg gtttagatgg atcaacctgg ctctggcggt
     1021 cggtgctgtc attggagcgc agacgtcttg ccagttcgtc ttcttgcaat accttattac
     1081 cgtcaaaaaa agtatcaggt ggttttacct gctctatttc gttgctatag tagcccatct
     1141 gcttaactcc tcgggcgttc caacttatag cttcaaccat gaaagtcttg tgaatattcg
     1201 catcattgag gcagttgacg cttgcgctcg ttcaactcgc ggttgatcag ctgcaggtta
     1261 tggtgttgca tgatgccggc ggcaagttcc ctggcctgct ctagttgata tgttgaaagt
     1321 ccgtccagat ccctgctctc caggtctggt ttgagcacgg tctggatttc ctgctcgatt
     1381 ctggcaatga gaccggaagc tctgcagatt agaagcagga gcgctggcaa aaacatggcc
     1441 ggggtgatcc tgagaaagaa cgtatctgtt gccttcggtc cggcgcaaag aatgcctgcg
     1501 gccaggcagg tggcaaggta caaaaagaag tattccatca atcggtatct acttagccat
     1561 ggaattccag tagaaggccg ctcattgtgg cagagtgtgc agctggtctg agaatataag
     1621 ccgatcagat ctaacagttc cctgatgcac cagccaagcc caagaagcaa gaccagtaaa
     1681 acaatcaaat ctgacgaaac agagccactg tatgtcattt gcggattagc cagactgata
     1741 agaccgcaag caaaaccgag aagtgagaaa agcaataagt acctggtcac cttcaagctg
     1801 gtcacttccg ggtgaaagat ggcgcagtgg cagaaagtga caagcaggtg gtagagcttt
     1861 tcttgcctag tcatttggtc ttcatccact tgctttaagc ttagtgtctg cctatcaacc
     1921 attttccatg ttggcggctg gaactgtcaa ttttgttctt ttttcagggg caagggcatt
     1981 ggatttcagg caggagcggg cataaatttt aagaaggcat tttgaggaag taacttgttt
     2041 gccaggcgcc tcaaggataa gaccgagttg aagtgcgaac cctattcgga atctcgcctc
     2101 agtcttgttc ctgcccctga ttccgatccc gaccggagcc aattagtaga gcgcgcttgc
     2161 cctgctactg ctgaacctgt cggtcgtttc tgctggctga aggacaggct ggccttcgta
     2221 tctatggcct cagcagcggc cttgcctttt gccttctacc aattcatggt ttcctacctt
     2281 aacttttcca tgagcggcgt gcagaccaac gagtttatgg agtctaccag cgggctgtct
     2341 tgcgcggcag tttgttttct catggtctgc acttatgaat atgccaggac ccttactggc
     2401 tacaaataca catggttctc cctgacttgg agaactgttg ctcttgcctg tgttgtcttt
     2461 actggatgga cggccggttt gagcggcgtg cttacagcaa taatggcttt ggctctagtg
     2521 tttgcggcct tttgtctggg gcggcttatc agagctgcag ttccgcccac cttcaatttc
     2581 cgcagatctg tctggaatat gggatggtgc tcggctccag gcttcctctt ccttttgtct
     2641 ttcgtagttt ctatgccaaa tcgggagcct tccggtacaa cctacgccaa tggtacagcc
     2701 tttgaattct tctttatgtc cgcattcttc ttcttgctac tgtccattcc cacatatgcc
     2761 gccatgcgtg ccgctcgcag ccgtgagacg gcacccctgg ctcttctggc cgggctttat
     2821 caagcaccat tttttcttgc caccatactt gaggcgagcc tggtagtttt taaacctggc
     2881 ggtctgacaa ttgccgtctc catatttttg tgcaatttaa ttgccggcgc cattttactc
     2941 ttcagtgccg ggcaggcagc tagagcaaac ggcgccgttc acgacaggcg aatcagcggc
     3001 tccaattaga atttagccga gcctatccga agtaccgccg gcccaggcga agctagtgcc
     3061 gccctgacca tagaagccct gtgcggcggc tactctggca ggcatgttgg gtttgccggc
     3121 tacttcgaat ttgtgttcga agacgtttgt agcttgttcg acggtgtggg tctgcatgag
     3181 ggctttgagg gcgcctttgt gggtggtttg caattcatgc caggcaaagt tgagctgggt
     3241 attgaggtcg tcggcaggca ttccatgttc ggcggcgtac ttgaagaggt cgcgcttcct
     3301 tgaaccttcc cactgggcga tgccgaaacc tggtccgccg ccgagttgcc tttggcttgg
     3361 atcgaatccc ggagcttcta catgaaaatt gcccatgatg ccggctacct gttctttgga
     3421 tagacccttg ctctcgaaga actgcctgat ttgttcccca ttactgagag aagcgctgtc
     3481 gcccttatgt cctgacagtt ccagaccggg cagatgggcg gcgcttgagt ccttgccggg
     3541 gctgaagtgg aaggcatcgg ctctggacca ggtgttttcc actatggagt ttatgccgtg
     3601 attttgctca ggctgcgcta agccattgtc gcttccgcgg tggtagtgtt cgtatgacat
     3661 agtaatgaat tcctgctgcc ggatagaaaa ggaaccccag ggaggttcgt gccattgagg
     3721 ctaaggccgg gagcataaaa tccgcatcaa accagtttgt atttgcttac tagagtttca
     3781 agcacgtcct ggatggccgt caggctctga gcggcttcat ctatctgttt gctggaatag
     3841 acatgctgct tggtggcttc ttgaatgcct tgcatggcag tcaccacctg gtctataccg
     3901 gtcaactgct cctggctggc ggcggcaatc tgcacggcgg cactggctgc ttccgctacg
     3961 gaatgagcca gggattcgat ggagcgaccg gcttccgctg attgtctgac ggcggtttct
     4021 accgtgcgac tgccttcttc gatggagagg gcggcctggg ctgctgcttg ctggatgtca
     4081 gagagtatgc gtcttacttc cgcggtggcc tgcttgctct ggttggagag ggctttcact
     4141 tcctgggcta ccacggcgaa gcctttgccc atttcaccgg cgcgggctgc ttcgattgag
     4201 gcgttgactg caagcaggtt ggactgttgc gacaattcgt ctacggaggc ggtgatttcg
     4261 gcgatggcct ggctcttgtc ggtgagtgtg gccatggaat tggcaatggc tctcatttgc
     4321 tctcgcaccg aggtcatctg atgaattgtg tcttccgagg cgttttttcc taccagggaa
     4381 atttgggccg cttgttgagc cagatctgcc acgaagcgtc ccttctgggt agccaccatt
     4441 gtggtctgac gcacttcttc tgctgaaacc gtggtttcgg tgacggtggc ggccatctgg
     4501 ctgcttgagg cggccgactg gctggtagaa acggcaatct gcttgacgga gttggtcagg
     4561 acgtctatgg catcggtaat ctggctcgta tcttccctca gttttatgac catggcatca
     4621 aaggcctggg tgagggaacc tatctcatct tcgtcgcggg ctttgggaat ggggaagttc
     4681 agttcaccct ttcctactct tgccgcagca ctggtgagag tttgcagggg ctctaccacg
     4741 tgggcggcca cgaaggtttt gattttataa gtggtatagg cggtggtgcc tatcaccaat
     4801 atataagcca cacccaggag tgtgttggat tgcttcaagg tggagaggtt ggaggagagt
     4861 tccgccggca aacccaggtc tgataaaccg gaaagcagtt gggagagttg ccaccactgc
     4921 cagcctatga aggctgtcag aatcagaaag atgaccgatg aggcaagcag ggtactgctt
     4981 aatttggcct ccaggctttt accgttatct tgatgcaagg gcttcacatt tctcctgtaa
     5041 gcaaagagac ttattgtaaa tctactattt gtcgataggc ctggttaaac aagcggatat
     5101 ctcgagcagg cggtggagag gttgagagca tattgacggc aagttccacc tgggcgacaa
     5161 atttttcctc cgatcccgat ttgaaaacta taccatcg
//
LOCUS       DAOQNZ010000070         5166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6611_length_5166_cov_3.831344, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000070 DAOQNZ010000000
VERSION     DAOQNZ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6611_length_5166_cov_3.831344"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PKW73_02070"
     CDS             complement(<1..1424)
                     /locus_tag="PKW73_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72096.1"
                     /translation="MPSPVFDRKKVLLAISLSLAVSSCLWQCAQVFKPVGAQGLSTIP
                     PKLNEQEQQFSLQFSETAGVRVNLFPYNQSVPGVPFLLKVNGRSVKGLTERQILKLIG
                     GGTAAIEVLTADGRVWQTKASPRAALPLGNGSKNFSTVVCSLYRYDGSNTIAGTRMGL
                     QMLGDDEYSNVVQLLLADSRCIAERADDEPETSGPSKASLAAACAAAELSVGLLPQAK
                     ANLRKLIANLQVPADNPPIFDRRIRQVFDAAVLFNLKDEARLIGENVSRSLLAGRYYQ
                     GDKRFGDDEENLFVQSYAKMLAKSSPAEALQFMRDYLSASKLPKGRFGPATPAWFAEF
                     YAACGDYAQAAQAYEKSLQEFARYRGSEGKFKYEQIGLAQSHCYALLRLSEFASAVGD
                     WDKAIAYLSQARLIYAENFSADTMKQMENLSQFCPSPALLEFSLAEAQLHKGDVSSTA
                     EAMNSLERGLRSGALRSGAPARDA"
     gene            1625..3469
                     /locus_tag="PKW73_02075"
     CDS             1625..3469
                     /locus_tag="PKW73_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72097.1"
                     /translation="MPKYAFSESEYKWCPQCGGNIRREAYYCRYCHKPIENRLLKQVA
                     VPPYTAIERASEWLPQLPAVKDKCHQDFTIRLGQTRPSTMAETLSGLVAAESNSGEQT
                     LCPHTLPEPQTAGLLMDILLSCYEQGENLPQVCEEPRLKLLELAPQEVVAEDELREME
                     QESGSQCRYCGEFILPASEECRFCQGSEAQAPQPILEPVEKKIDTALLKAVLFYEYAW
                     RKLHKEDNPLEQLFDESTLNSEELAVELARQRAGVYDMPTSRFCRRMFQLNLESYYSK
                     ERLSLMEIADLGSALESKKVAKNTEAQIVLEHALRRTEGKDELMMERSTILNYLCLLF
                     YNKDETKHNLYKAMARSCEIFGMDEQTRALTEYSYQMLDALAKDSRLLEGNPEYHLDK
                     LEEMLSEDPTNPSGNFLGKLAAVLPEGNAFFNAIKSSLSNIVSSSRLSVEAHTAMENE
                     DFETAESKLREALKLESEDSVTAGSNRLTILLDLAQVKQKTNDADSAESILQEAMKLA
                     TELEEVNPVLFRFLLSKTRQSLADFLKAAGRYEEAEREFQAVFKLQEQIMAESARRYG
                     TTSEAWVGKQIENLENYAQLLTAMKRDADLQKVESEIARLKQEGKKDS"
     gene            complement(3555..>5166)
                     /locus_tag="PKW73_02080"
     CDS             complement(3555..>5166)
                     /locus_tag="PKW73_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNA72098.1"
                     /translation="HWPSRLDVELPLCSTDLGAPSGDPNDRCAMVGFIEDYFQALVFR
                     KRLHLQTLPTAGKISEEDWRKQREQLEQVMGYHPAAHVPAYDLFASEFGICDRWYSSY
                     PGNTWVNRTISLCGKPGKRQKLTANDFRSKEEFKEWEEQQFHQEHGLFITNNDMPWDE
                     ESFFRYMDKTKKPDGSKVDWAFYAQDMPSLLCVDSSYASGFRNLMNGVPNRIKSIKKF
                     FKDAEDGKLPEVFWIDPNFVDLGDIRETLGNREKNPVVEHWVEGERGKRDREREKDAF
                     GEMILDVNTANDDHPPADISHGQHFALEVFNALLKSPNWHSTMLIIVYDEHGGFFDHV
                     RPPYLDQLQNPVEPESPAFRSLGARVPAIVISPWIGRGLVSHTSFEHSSIIKTIMLRF
                     LGTDTGISERVLKAHHLGGLLSEKSPRFSTVYSSGVGSSAERYRASGSENRDERSARS
                     LASRPAGKEPALPVGGKSPRPRNEEDQEKRLARLKSAVQGTAAALRLYQGRDRMLRKG
                     KRRPLTDLGEQMTSARKQVFERLFGKDG"
BASE COUNT         1375 a         1363 c         1298 g         1130 t
ORIGIN      
        1 aaggcgtccc tggcaggtgc acctgaacgc aacgcaccgc tccgcaggcc acgctccaga
       61 ctattcatag cctcagcggt agaactaaca tcgcctttgt gcaactgagc ctcagccagg
      121 gaaaattcca agagcgccgg gctcggacaa aactgagata aattctccat ctgcttcatg
      181 gtatcagcag agaaattctc ggcatagatg agcctggcct ggctgaggta agctatagct
      241 ttatcccaat ctcccacagc ggacgcaaac tctgacagac gaagcagagc gtagcagtgc
      301 gactgcgcaa gcccgatttg ctcatacttg aatttaccct cgctgcctcg atatctggca
      361 aactcttgaa gcgacttctc gtatgcctga gcggcttggg catagtcacc gcaagcagca
      421 tagaactcag caaaccaagc aggcgttgcc ggtccgaacc gacctttcgg gagcttactg
      481 gcggacaggt agtctctcat aaactgcaaa gcttcagcag gcgaagactt cgccaacatc
      541 ttagcgtaag actgaacaaa gagattctct tcatcgtcgc cgaatcgctt gtcaccctga
      601 tagtaacgcc cagccagaag tgaacgagag acattttcac caatgagccg tgcttcatcc
      661 ttcaaattaa agagaacagc ggcatcaaag acctgtctaa ttcggcggtc gaagatgggg
      721 ggattatccg ccgggacctg caggttggcg ataagcttgc gaaggtttgc ttttgcctgg
      781 ggaagcagac ccacggacaa ttcggcggca gcgcaagctg cagctaagga agcttttgag
      841 ggtccactcg tttccggctc atcgtcggct cgctcggcta tacagcgact atccgccagg
      901 agcaattgaa caacgtttga atactcgtca tccccaagca tctgcaagcc catgcgagtt
      961 ccggcgattg tgttgctgcc gtcatatcta taaagggagc agacaaccgt agaaaagttc
     1021 ttactaccgt tgccgagagg aagcgccgcc cgaggagatg ctttcgtctg ccagactcgc
     1081 ccatccgccg tgagtacctc gatagcagca gtgcctccgc caattagctt gagaatctgc
     1141 ctttcggtca agcccttcac ggagcggcca tttactttca aaaggaaagg gacaccgggc
     1201 accgattggt tgtaagggaa taggttcaca cgcacaccgg ctgtctcaga gaactggaga
     1261 ctaaactgct gctcctgttc attaagcttt gggggaattg tggatagccc ctgtgcaccg
     1321 accggcttga acacctgcgc acattgccag agacaagaac tgactgccag tgacaaactg
     1381 atggcaagaa gcactttctt tctatcaaac actggtgatg gcatttgctg cgcttgatcc
     1441 ttactctcga gaatgcatac gtcgaactct accaatgggt gatcctagca gaacagtgag
     1501 acaagtagaa tggtctggaa gccggcaaat cgacttagct actgactctc tagtctgttt
     1561 gaccctacca caaaagtgga atagccgaca aagtcacctg cttctcagtt ggaaaagcta
     1621 aaccatgccc aaatacgcat tttctgaaag cgagtataag tggtgcccac aatgtggcgg
     1681 caatattcgc cgcgaagcat attattgccg ttattgtcat aaaccaattg aaaaccgact
     1741 tctaaaacaa gtagccgtac cgccttacac cgctatcgaa agggcaagcg aatggttgcc
     1801 gcagttaccg gctgtaaaag ataaatgcca ccaggacttt accatcaggc tcgggcagac
     1861 aagaccctca accatggcgg aaactctttc ggggctggtc gcagccgaaa gcaactccgg
     1921 tgagcaaaca ctctgcccgc acacgctgcc ggagcctcaa acggcgggtc ttctgatgga
     1981 cattctgcta tcttgctacg aacagggcga gaacctgcct caagtgtgcg aagaacccag
     2041 actgaagctc ctggaacttg caccacaaga agtggttgcc gaagacgaac tgagagaaat
     2101 ggaacaagaa agcggtagtc agtgtcgcta ttgcggagaa tttatcctgc cagccagcga
     2161 ggaatgtcga ttctgccagg gcagcgaagc acaggcacca cagccgatac ttgagcctgt
     2221 tgagaagaag atcgatacag ccttgctcaa agccgttctc ttttacgaat atgcctggcg
     2281 caaactacat aaagaagaca accctcttga gcaactattc gatgagagta ccttaaattc
     2341 agaagagctg gcggtggaac tggcaaggca acgagccggt gtttacgaca tgccgacctc
     2401 acgcttctgc cgacgcatgt tccagctaaa tcttgagagt tactactcaa aggaaaggct
     2461 ctcacttatg gaaatagccg accttggttc ggccctcgaa tcaaagaagg tagcaaagaa
     2521 cactgaagcc caaattgttc ttgaacacgc actgagaaga acagaaggca aggatgagct
     2581 gatgatggag agaagcacca tcctgaacta cctgtgcttg cttttctaca ataaagacga
     2641 gacaaaacat aatctctaca aagcgatggc acggtcctgt gaaatattcg gcatggacga
     2701 gcaaaccaga gcattgacgg aatacagcta tcagatgctg gatgcccttg caaaagacag
     2761 cagacttttg gaagggaacc ccgaatacca tctggacaag ctcgaagaga tgctttccga
     2821 agaccccact aatcctagcg gaaactttct cggcaaactc gccgcagtgt tgccggaagg
     2881 aaacgcattt ttcaatgcca tcaagtcttc tctgagtaac attgtaagtt cctcgcgttt
     2941 aagcgtcgaa gctcatacag caatggaaaa tgaagacttt gaaacggctg agagtaaact
     3001 cagggaagcc ctcaagttag aaagcgaaga ctcggtgact gctggcagca accgtctgac
     3061 catcttgcta gacctagccc aggtgaagca gaaaacaaac gatgcagact ccgccgaaag
     3121 cattctgcaa gaagcaatga agctagccac agaactagag gaggttaatc ctgttctatt
     3181 caggtttctg ctgtcaaaaa caagacagtc cctggccgac tttctgaaag ctgccggtag
     3241 atatgaagaa gcagagcgag aattccaggc agtcttcaag ctgcaagagc aaatcatggc
     3301 ggaaagcgcg cgccgatatg gtaccaccag tgaggcgtgg gtcggcaagc agatagagaa
     3361 cttggaaaat tacgcccagc tactgactgc gatgaaaaga gacgctgatt tgcagaaagt
     3421 tgaatcggaa attgcccggc tcaagcaaga gggcaagaaa gactcctaga agtcaattat
     3481 tcaagcgact aaatccagcg tgaggaccac cgcccgtttg gcaggaacca gccaagcgat
     3541 aaatccatta gccattagcc atccttacca aagagtcgct cgaatacctg ttttctggca
     3601 ctggtcatct gctcgcctag atcggtcagg gggcgacgtt tgccttttcg cagcattcga
     3661 tcacgcccct gatacaaacg aagcgccgca gctgttccct gaaccgccga cttaagtctg
     3721 gcgaggcgct tctcctggtc ttcctcatta cgcggacgtg gagacttgcc tcccacaggt
     3781 agagcaggct ctttaccggc tggtctagat gccagactcc tggcgctacg ctcatcacga
     3841 ttctcgctcc cgctggcacg gtacctttcg gcgctggaac cgacaccgga agaataaaca
     3901 gtcgaaaagc gcggcgactt ctcactcagc aatccaccga gatgatgagc cttcagcacc
     3961 cgctcgctga taccggtatc ggtgcccaga aagcgcagca ttattgtctt gattattgag
     4021 ctatgctcaa aggaagtgtg agaaaccagc ccccgcccta tccagggaga aatgacaata
     4081 gcgggcacgc gagcccccag gcttctgaaa gcagggcttt ctggttccac cggattttga
     4141 agctgatcta gataaggtgg tcttacatga tcaaaaaagc cgccgtgctc atcgtaaacg
     4201 atgatgagca tggtgctatg ccagttaggg ctcttgagca gagcattaaa gacttccagc
     4261 gcaaaatgct ggccatggct tatatcggcc ggtgggtggt catcattggc ggtattgacg
     4321 tccaggatca tctcaccaaa ggcgtctttc tcgcgctcgc gatctctttt gccacgctcg
     4381 ccttccaccc aatgttctac taccgggttc ttctctctat ttcccagggt ctctcgaata
     4441 tcgccaaggt ccacaaagtt aggatcaatc cagaacacct ccggcaattt gccgtcttcg
     4501 gcatctttga agaacttttt gatggacttg atgcgattgg ggacaccgtt catcagatta
     4561 cgaaaaccgg aagcatagct tgagtccacg caaagaagcg agggcatatc ctgcgcgtag
     4621 aaagcccagt cgaccttact gccatcgggc ttcttggtct tatccatata tctaaagaat
     4681 gattcttcat cccagggcat gtcattgttt gtgatgaaaa gcccgtgctc ctgatgaaac
     4741 tgctgctctt cccattcctt gaactcttcc ttactgcgaa aatcgttggc agttaacttc
     4801 tgccgcttgc ccggcttgcc gcaaagggat atggtgcggt ttacccaggt gttgcccgga
     4861 taggaagagt accagcgatc gcaaatgccg aattcactgg cgaagagatc ataggcaggc
     4921 acgtgggcgg ccggatgata gcccattacc tgctccaact gttctctttg ctttctccag
     4981 tcttcctcgg atattttgcc cgcagttggc agggtctgca aatgcaagcg cttcctgaaa
     5041 acgagagctt gaaaatagtc ttcgataaag ccgaccatgg cacagcggtc gttgggatcg
     5101 ccgcttggtg caccgagatc ggtggagcag aggggcaatt cgacgtctaa tctgcttggc
     5161 cagtga
//
LOCUS       DAOQNZ010000071         5159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6629_length_5159_cov_3.738245, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000071 DAOQNZ010000000
VERSION     DAOQNZ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6629_length_5159_cov_3.738245"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..1277
                     /locus_tag="PKW73_02085"
     CDS             225..1277
                     /locus_tag="PKW73_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNA72099.1"
                     /translation="MTTFSTDSDCHGGPDPSQNPNHKGFADTRRHLVFHPQYQTDLPA
                     YGLNQPFALDRGAMVLRRLTMELGQTPAYETPRPLERQQVTRVHSEAYLESLSQSSTW
                     LEIFELKEELDALGRLPGAVKPFHTILDDFLLKAGGTLLSARKAIAGGMCANLGGGYH
                     HAFPDQGRGFCAINDVAITVHEILREKLAERVMVVDVDFHQGDGTARCLSGRKDVFTF
                     SIHSHEGWPEEKSVSSKDIAVAEKDQTRYLDMLQEGLAASVQEFQPDFVLLVDGSDAY
                     EKDVLPGTKFLKLPLSVMQKRSEYIIDLFAERKIPLSLVFAGGYGPDVWEVHYHTVRQ
                     LLARSGVTFGAAARIT"
     gene            1255..2115
                     /locus_tag="PKW73_02090"
     CDS             1255..2115
                     /locus_tag="PKW73_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNA72100.1"
                     /translation="MQRRESLKESPVKDAVVALPFLKWAGGKGRLLSQYAGLFPKDFE
                     NYFEPFLGGGAVYFHLSQNRKFEKAYISDYNPELTNCYKMVQKEPKKLLALLSKMRND
                     KHLFYKERARDVSQLTDLERAARLIFLNRTCFNGLYRVNSQGQFNVPFGKYKNPRIAD
                     AGNLMAASAVLQNASIHTGSFETLVSKAKSGDFVYFDPPYQPVSRTASFTSYTKNSFN
                     LADQARLAQLARRLQERGVLVMVSNSDTDEIRDLYRDFDLTIVKAARAINCRADGRGV
                     VNELVLRSYR"
     gene            2477..3790
                     /gene="serS"
                     /locus_tag="PKW73_02095"
     CDS             2477..3790
                     /gene="serS"
                     /locus_tag="PKW73_02095"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNA72101.1"
                     /translation="MLDINYIKREPELFKKTIRDKGINLDLDELLACYEELVLLNKEI
                     NGLRERRNAIAKEFGMLKEREVVGADAEALNGARKAGLPLAEGKEVALHLKALERRQT
                     DLEDRYRRLLLLVPAPVLPAVPVGNSDLDNVELYRRGELPQFDFQPLDHLALVERLGL
                     VDFVGARAAAGARAYALLGDLMLLEMAVQRYAVDYLVGRGFQPVAPPVMVRQEAMEGT
                     GYFPLGLENAYELKKDDLFLTGTCEVGLVAMHRNRLFEKQELPRRLVGLSTCFRREAG
                     AAGKDTRGLFRVHQFQKVEQVAFTVAEQEAGLAMHYELLENAEAILRALELPYRVVQV
                     CTGDIGQGQVFKHDIETYMPARGGYFETHSCSFLADFQARRLNMRYKEGGKRPYVYTV
                     NNTAAATPRLLIALLENHQEKDGRVRIPGCLQPYMGRTHLEPRFK"
     gene            complement(3919..5127)
                     /locus_tag="PKW73_02100"
     CDS             complement(3919..5127)
                     /locus_tag="PKW73_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72102.1"
                     /translation="MNLVKKQRENRYRLGELFLETGLVDVSAVSEGLSIAKRTSFPIG
                     RVLVMTGWLDDHDVNCALELQGLLREGTIDNSLAKDLLRFCHLNKVDINESFRLNGLS
                     RSGESPQTRLGRLFLAAGVLDENQLAQGARESQRHNTTLGGALLMLRFVSAKTLEGAL
                     NLQIMLRDNKVNFAEAIAFCKEMHERQVSLREVLGDNGKLVRSNNAAPRLGEFLISAD
                     LVQPKDVLTACEVGTEEDNNIGRVLMNHGHISELALEAALKLQHMIQNRVLTYRRAIK
                     LLRLVCRLGAPLEQIIEESESLDEVFKLLRRAAIVPERLVRDVAAEIIDFEDTVAEAM
                     LARGYVNPMHARIGLACLERVKQGEICETKAAFLVHHCCTKPGEEMDMFCRINWQELK
                     RIQIRQDLLA"
BASE COUNT         1157 a         1288 c         1438 g         1276 t
ORIGIN      
        1 ccatccgccc tcttggaaag cgaggaggcc gcggcattgc tgtagaaagc gccacatgac
       61 aatttggcaa tcttttgtcc gacaagatat ggccaactcg cacatcttga caacaacacc
      121 aagtgcagtg gcgatcggtc tttctgcatc tgctctgtaa aggcccatga tggggctgat
      181 aaatagccgc cactgccagg atcgcccttg ctatactgac cgggatgact actttcagca
      241 cagattcaga ttgccatggc ggaccggacc cgagccagaa cccgaatcac aaagggttcg
      301 ccgatactcg ccgccatctg gtgtttcatc cccagtacca gactgacctg ccggcctacg
      361 gattgaatca gccttttgcc ctggatcgag gcgccatggt cttgcgccgt ttgaccatgg
      421 aattgggtca gacacctgca tacgaaacgc cccgtccgct ggagcggcag caagtcaccc
      481 gggtacacag cgaggcctat cttgagagcc tttctcagtc cagtacctgg ctggaaattt
      541 ttgaactgaa agaagagctg gatgctcttg gtcgcctgcc tggtgcggtc aagcccttcc
      601 acaccattct ggacgacttt cttctcaagg ccggcggtac tctcctttct gcgcggaaag
      661 ccattgccgg cggcatgtgt gctaatttgg gcggcggcta tcatcatgcc tttcctgatc
      721 aaggtcgggg cttttgtgcc atcaacgatg tagcaataac cgtgcacgag attctcaggg
      781 agaaactggc tgagcgtgtc atggttgtcg atgttgactt tcaccagggc gacggaacag
      841 cccgctgtct gtccggtcgc aaagacgttt tcacattttc cattcattcc catgaaggct
      901 ggccggaaga gaagagtgtg agcagcaaag acattgccgt agcggaaaaa gatcagactc
      961 gttacctgga tatgttgcaa gagggtctgg ctgcttccgt gcaggaattt cagcctgatt
     1021 ttgtgctctt agtagacggc agtgatgctt atgaaaagga tgttttgccg ggcaccaagt
     1081 ttctcaagtt gccactttct gttatgcaga aacgcagcga atacatcata gatcttttcg
     1141 ctgagcggaa aatccctctc agtctggtgt ttgcaggtgg gtatggccct gatgtctggg
     1201 aagtgcacta tcatacggtc aggcaattat tagcaaggag tggtgtcacc tttggtgcag
     1261 cggcgcgaat cacttaaaga atctccggtg aaggatgctg ttgtcgccct gccctttctc
     1321 aaatgggcag ggggcaaagg tcgcttgctg agccagtatg ccgggctctt tccgaaagat
     1381 ttcgagaatt acttcgaacc gtttctcggc ggtggagccg tatactttca tttgagtcag
     1441 aatcgtaaat tcgagaaagc ctatatctct gattacaatc cggagctcac caactgctac
     1501 aaaatggtgc agaaggagcc gaaaaaacta ttggcgctgc tctccaaaat gcgcaatgac
     1561 aaacatctct tttacaaaga aagagcccgt gatgtttctc agcttaccga cttagaacga
     1621 gctgccagac tgatttttct taatcgcact tgctttaacg gtctttatcg agtcaacagc
     1681 cagggacagt tcaatgtgcc tttcggcaag tataaaaacc cgcgcatagc ggatgccggc
     1741 aatttgatgg ctgccagtgc ggttttacag aatgcttcga ttcatactgg ctcctttgag
     1801 accctcgtgt caaaggcaaa gagcggcgac ttcgtttact tcgatccgcc ctatcagcca
     1861 gtcagtcgca ctgccagctt tacgtcttac accaagaaca gcttcaatct ggccgaccag
     1921 gcgcgcctgg ctcaactggc gcgccgcctg caagagcggg gtgtgcttgt tatggttagc
     1981 aattccgata cggatgaaat cagggatttg tatcgtgatt ttgatctcac tattgtaaaa
     2041 gcagccaggg ctatcaactg tcgagccgac ggacgcggtg tggtcaacga gttggttttg
     2101 cgaagctaca ggtgattgtt gcttcctcaa ttattcgcaa agaagcgttg acaaaaattt
     2161 ttctccaggc aaactagagg tatgttgata aatcggcaca ccgctaacct ctcactttca
     2221 tctcgctctt catcatcaga gcttaatgaa ggggctctgc cgtgccgggt ttgtcacagc
     2281 gcaaaggtta tttcatggct ttcatcttgt tcatgagttg agactatcgg tcttatagca
     2341 agatagaagt taaagacaag caggttcaat taattggtta attagttgag ctgatgcgcc
     2401 aagtacgtat gccaggcagg cgagacccct cggggctcgc ctgctgcttt ttacacatgt
     2461 tttgaggaaa tgaaagatgt tggacatcaa ttacataaaa cgggaaccgg aactttttaa
     2521 gaagacaatt agagacaagg ggataaacct tgatttggac gagcttttag cttgttacga
     2581 agaactggtg cttttgaaca aggaaatcaa cgggctgcgc gaaagacgga acgctatcgc
     2641 aaaagagttc ggcatgctga aagaaaggga ggtggttggg gccgatgcag aggcgctgaa
     2701 tggggctagg aaagcgggtt tgccgttggc agaaggcaaa gaggtagccc tgcatttgaa
     2761 ggcgcttgag agaaggcaga ccgatttaga ggatcgctac cgacgtttgc ttcttttggt
     2821 tccggctccg gttctgccgg ctgtgccggt cggcaacagc gatttagata acgtcgagct
     2881 ttaccgcaga ggcgagcttc cgcaatttga cttccagccc ctcgatcatc ttgcccttgt
     2941 ggaacgactc ggattggtcg attttgtcgg tgcgagggca gcggctggtg cccgtgctta
     3001 tgcgcttctg ggtgatctga tgctcttgga aatggcggtg cagcgctatg ctgtggatta
     3061 tcttgtcggg cgcggttttc aaccagtggc accgcctgtg atggtacggc aggaggccat
     3121 ggaaggtacc ggctactttc ccttgggtct agagaatgcc tatgagctga agaaggatga
     3181 tctttttctg accggtactt gcgaagtggg attggtggcc atgcatcgca atcgactctt
     3241 tgagaagcaa gaactgccca ggcgccttgt tggtctgagc acctgtttca ggcgggaggc
     3301 cggtgcggcc ggcaaggata ctcgtggact gtttcgcgtg catcaatttc aaaaagtgga
     3361 gcaagttgct tttacggttg ccgagcaaga ggctggtctg gctatgcatt acgagcttct
     3421 cgagaatgcc gaagctatcc tcagggcgct tgaactacct tatcgggtgg tgcaggtatg
     3481 caccggcgac atcggtcagg gacaggtctt caagcatgat atcgagactt atatgccggc
     3541 ccggggcggt tattttgaga cccattcctg cagtttcctg gctgattttc aagccaggcg
     3601 tctgaatatg cgctacaaag agggcggcaa gcgtccctac gtttatacgg tcaataacac
     3661 ggccgccgcc acaccgcgcc tgctcatcgc cctgctggag aatcatcagg agaaggatgg
     3721 tcgggtgcgc attcccggct gtttgcagcc ctatatgggc cgtacccatc tggagccgcg
     3781 cttcaaatga ggtttgccgg cgcggcatgc tttaaggaga ccgaagcaac ttcggtctcc
     3841 ttaattacct tactaaaaag tctctacaaa agggtctcta ctaagaaggg cgagggtcgt
     3901 gaacccaagc ggctgcaatc aggccagcag gtcctggcga atctgaatgc gcttcagttc
     3961 ttgccagttg atgcggcaga acatatccat ctcttcaccg ggtttggtgc agcagtgatg
     4021 caccaggaag gctgctttgg tctcgcagat ttcgccttgc ttgacgcgct ccaggcaggc
     4081 cagaccgatg cgggcgtgca tggggttgac atagccgcgg gctagcattg cttcggctac
     4141 ggtatcttca aagtcgatga tttcggccgc cacatctctc accaatcgct ccggcacaat
     4201 ggctgctctt ctcagcagtt tgaatacttc gtcgaggctc tccgattctt cgatgatttg
     4261 ctcaaggggc gcacccagtc tgcaaaccag gcgcaagagc ttgatggcac ggcggtaagt
     4321 gagaacgcga ttctgaatca tgtgttgaag cttgagagct gcttcgagag ccagttctga
     4381 gatgtgacca tggttcatga gcacccggcc gatgttgtta tcctcttccg tgccgacttc
     4441 gcaggcagtc agtacatctt tgggctgtac caggtcggcg gaaatgagaa actcgcccag
     4501 tctgggcgcg gcattgtttg aacgaaccag tttgccgttg tcacccagca cttccctcag
     4561 gctcacctgg cgttcatgca tttctttgca gaaggcgatg gcttcagcga aattgacttt
     4621 gttgtcccgc agcatgatct gcaggttgag agcgccttcc agtgtcttgg ccgagacgaa
     4681 gcgcaacatg agcagggcgc cgcccagggt agtgttgtgg cgctgggatt cacgggcacc
     4741 ctgtgccaac tggttttcat ccaatacgcc ggcggctaga aacagcctgc ccagacgggt
     4801 ctgggggctt tcgccagagc ggctcaaacc gttcaggcgg aaggactcgt tgatgtctac
     4861 cttgtttagg tggcagaagc gcaggaggtc tttggccagg gaattatcta tggtgccttc
     4921 ccgcaagagg ccttgcagtt cgagggcgca gttgacgtca tgatcgtcga gccagccggt
     4981 cataaccagc actctgccga tggggaagga ggtgcgcttg gcgatggaga gtccttccga
     5041 tacagccgaa acgtccacca gtccagtctc aagaaaaagt tcgccgaggc ggtagcgatt
     5101 ttcacgctgc ttcttgacta aattcatgac attctcctta ggtctgcaaa ttctttatg
//
LOCUS       DAOQNZ010000072         5133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6676_length_5133_cov_3.881843, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000072 DAOQNZ010000000
VERSION     DAOQNZ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6676_length_5133_cov_3.881843"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKW73_02105"
     CDS             complement(<1..210)
                     /locus_tag="PKW73_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA72103.1"
                     /translation="MNSEPISILIVEDQELIRLGLRLSFQRQEGFVVVGEAADGLSAI
                     KKAIELRPQLAIVDIGLPGLNGIEAG"
     gene            complement(276..1946)
                     /locus_tag="PKW73_02110"
     CDS             complement(276..1946)
                     /locus_tag="PKW73_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72104.1"
                     /translation="MKSSLYLFWQRVCHSSLSPLEVLTCLLLSFLLGQTIIHQASIDT
                     DMAVNLSTALALLDGSRLYLDNFNPAWPPALFLILPAAALARALHIYPVLFLKLFFYL
                     LACLAILAMMWGVRLRRATALESCLLPLAAPFSAAFLLLTSAQFMGLENFPNGGQFFP
                     LMVLPLLILQAKDNSAPETPPALSALTYFLAAVAICFEPSFVLYYLLIVSFADLRNLK
                     RNCLPLLLPALLLLIFAVSYEGAWQNYLDWSVLINQLNFLGFNDYLYWMDKSPDLRKP
                     VYGFVMLLVLSAPLLVRSALSRQLAALACFGFAYFISSQTLLGQFTLPMLFFAGLSGL
                     GALVFLCRVARLRGPLRKMRPLSRRLSARPAILVCLFLLAGSSLLQSLSREETVTIDD
                     GAGTKAVVRCSDLSIFARTVLNESRAGEPIYVYCWQARPGFPLTAQLMRKTSRFPYLY
                     PFMAFHHGGAEASACGVLNDPERLPKLESKFYSLLQAELSAANRPALIFIEDGDIRQQ
                     FDDRGLVKLLDSDYSMLASFTFSPDERKGHPSFEYPGYMNAFAVFKHR"
     gene            complement(1950..3512)
                     /locus_tag="PKW73_02115"
     CDS             complement(1950..3512)
                     /locus_tag="PKW73_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72105.1"
                     /translation="MKAAVSLILVFVLCLRMLVSILSHPLRIGWDPALHLQCAELITR
                     GKIPYVDMFDVNPPLIWYLDTIPALIAAALHWPQTLTFNLCLLVVIAYCGLSLSYIIN
                     KKPAFEGQDMVMLGILAGALFFNFQLRYDFGQREEIFVLLYLPYLFLRYSRYRGAPIG
                     SREAIFFGVAGALGICLKHYFLLVAVLVELAMLLAWGGLKKLLAPENYAALAFALLYL
                     GHFLLAPTAMKDNYFNFLVPAFAKGYGFWDTCLANSLAAPDKRGVFYLTAVCATFALM
                     LCRRVPIFLPLSVFTLSSIIPYLMQFKGWNYHDIPVFAGASLLAGGLLGYLLGQTARL
                     VKVRGMTEHLPLMVVVATSGFCFMNGMEEVASVKGERQFDLSRLGYKGSSPWSDIDSP
                     FTDYLLKYTRPGDAVIFISNGVSPGYPLLTQFALAPGSRHLHCCILSVFQFIKEAEPD
                     NAEARKLLSREPEVIEQYGIDIAAKKPVLIFIQEAPVISYLNPYDFFGKYMKSYERLG
                     SAANFSVYRRVD"
     gene            complement(3518..4984)
                     /locus_tag="PKW73_02120"
     CDS             complement(3518..4984)
                     /locus_tag="PKW73_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72106.1"
                     /translation="MLGSPATAQDNDLTFQSGLRCFNAKDYKGAINYFLAAEKESAYD
                     FRPVYYQALCHHRMGQVGAARQLYGTLINRFPDSEGAELARKALSINTGSPNTTFARG
                     SGLQNMQVSQDVLPKSTVAKAEEIDGKPTVIVKVGGSDIKMIVDTERQESLLGSNVAV
                     KARLNVIALEGRPGKSAEDGVYSCFDVTVGGIKRPQLPLYFSNKDKDAAVLGADFLNG
                     YKVAFNKEKGEVTFERIAGAGDPFGAGMALYKKNKFREALPLLRSAVANKPQDPRALY
                     CLANCLQKAGYIEEAKSTYRKVFKRFSNSEAGFLSRVALEQMDPSFRQEMRALETAQR
                     SNSGLNKMKKEEWTEVPYVIENSRYRVKVYFDNQPMEAYFEFQDSACRFSADMIRQVD
                     SAYLNDNGNVRTESSEGPESNQAIITTSWNIKLKSVRMGKIEARDVPGQVIETRGVRG
                     WSPDYGLLPRPTVCGYVLKGYRFDVDTTRKVIKIWRPQ"
BASE COUNT         1450 a         1326 c         1320 g         1037 t
ORIGIN      
        1 accggcttca ataccgttca aaccaggaag accaatatca acaatagcca attgcggtcg
       61 cagttcaatg gcttttttaa tggcggaaag tccatcagcc gcttcaccta ccaccacaaa
      121 gccttcttgc cgctgaaagc tcaggcgcag cccgagcctt atcagctcct ggtcttcaac
      181 aattagaatg gatattggtt ccgagttcat aaatccactg ccggcaaact tccctcatta
      241 aagacacaag cacactatac ccaagtaatt tcccttcatc gatgtttgaa tacggcaaag
      301 gcattcatat agcccggata ctcgaaggac ggatgcccct tgcgctcatc cggcgaaaat
      361 gtaaacgaag caagcatact gtaatcagaa tcgaggagtt tgaccagtcc ccggtcgtca
      421 aattgctgcc tgatatcgcc atcttctatg aagatcaagg caggtctatt ggccgcggat
      481 agttcagcct gcaagagaga gtagaatttc gactcaagtt tcggcagccg ctcggggtca
      541 ttcaaaactc cacaagcact ggcctccgcg cccccatgat gaaaggccat aaagggatac
      601 aaatagggaa agcgcgatgt cttacgcatc aattgagcgg tcaaaggaaa gcccggtctt
      661 gcctgccagc aatagacgta gattggctcc ccggcacgtg attcatttaa gacggtacgg
      721 gcaaagatgg aaaggtcaga acaacgcacc acggccttgg tgccggcgcc atcatctatg
      781 gtgacagtct cttcccggga aaggctctgt aagagactgg aaccagccag aagaaagagg
      841 cagaccagga tggccggtct ggcggacaga cgccggctaa gcggcctcat tttcctgaga
      901 gggccacgca ggcgtgctac ccggcagaga aagaccagag cgccaagacc gctcagaccg
      961 gcaaagaaaa gcatgggtaa agtgaattgc ccgagcagag tctgcgaact aatgaaatag
     1021 gcaaaaccga aacaagccag tgccgccaac tggcgagaga gcgccgagcg caccagaagc
     1081 ggtgcggaga gcaccagcag catgacaaag ccgtataccg gcttccttaa atccggcgac
     1141 ttatccatcc agtaaagata gtcattaaag cccaggaagt tcaattgatt tatcagaacc
     1201 gaccagtcca ggtagttctg ccaggctccc tcgtaagaaa cggcaaatat caaaagcaga
     1261 agagccggca agagaagagg caggcagttt ctcttcaggt ttctcaggtc ggcgaaagag
     1321 acaatgagca ggtagtacag cacaaaactt ggttcgaaac aaatagcgac ggcggccagg
     1381 aagtaggtta gcgctgataa agccggcgga gtttccggtg cggaattgtc cttagcctga
     1441 agaatcaaca gcggcaaaac cattaaggga aagaactgtc cgccattagg gaaattttcc
     1501 agtcccatga actgagccga ggtaagcagc aaaaaggccg cactaaaggg agccgctaaa
     1561 ggcagaaggc aggactcgag ggcagtggcg cggcgcaacc ggacgcccca catcatggca
     1621 agaatagcaa gacaggccag taagtagaaa aagagcttca aaaaaagcac cgggtaaata
     1681 tgcagcgctc tggccagggc agcggccggc aaaatgagaa acagtgccgg cggccaggcc
     1741 ggattgaaat tgtccaggta aagcctggag ccatcaagta gagccagggc agtactgagg
     1801 ttgacagcca tatccgtatc gatgctggct tgatgaataa tcgtctggcc aagcaaaaaa
     1861 gaaagaagca ggcaggtgag aacctcaagg ggactgagag aagaatggca aactctttgc
     1921 caaaatagat acaggctgct cttcatagat caatcgaccc gcctgtagac actaaaatta
     1981 gcagccgagc ctaagcgctc atatgacttc atatacttgc cgaagaaatc atagggattt
     2041 aaataagaga ttaccggcgc ttcttgtatg aagatcagaa ccggtttctt ggccgcgata
     2101 tcgattccgt attgctctat cacttccggc tcgcgactga gcaatttacg ggcctcggca
     2161 ttgtcaggct ccgcttcttt gatgaactgg aagaccgaaa ggatacaaca gtgcagatgc
     2221 cgcgaaccgg gagccagtgc aaattgagtc aagagcggat agccgggaga aactccgttg
     2281 gaaatgaaga tcaccgcatc acccggtctg gtgtacttca ggaggtagtc ggtaaaggga
     2341 gagtctatat ccgaccaggg agaagagccc ttatagccca gccggctcaa atcaaactgc
     2401 ctctcgccct tcacggaggc cacctcttcc ataccgttca tgaaacagaa cccggaagtt
     2461 gccaccacca ccatcagagg caggtgctcg gtcataccgc gcaccttcac aagacgagcc
     2521 gtctgcccca gaagatagcc gagaagaccg ccggcaagca gactggcccc ggcaaagaca
     2581 ggaatatcgt gatagttcca gcccttgaac tgcatcaaat agggaatgat ggaggagagt
     2641 gtgaaaacgg aaagcggcag aaatatcggc actcttcgac aaagcataag ggcaaaagtc
     2701 gcacagacag cggttagata gaatacgcct cgtttgtccg gcgcggcaag actgttggcc
     2761 agacaagtgt cccaaaagcc gtaacctttg gcaaaagccg gcaccagaaa attaaagtag
     2821 ttatctttca tagcggtcgg cgccagcagg aagtgcccca ggtatagaag agcaaaggca
     2881 agagcggcat aattctccgg agccaaaagc ttcttcaagc ctccccaggc aagcaacatg
     2941 gccagttcca ccagtaccgc caccagaaga aaatagtgct tcaagcaaat acccagagcg
     3001 cccgctactc cgaagaaaat agcttccctg ctaccaatcg gcgcgccgcg atagcggctg
     3061 tagcgcaaga agagataggg caaataaagc agtacaaaaa tttcttctct ttgcccaaag
     3121 tcataacgca attgaaaatt gaagaaaagg gcgccggcca gaatgccgag cataaccatg
     3181 tcctgcccct cgaaggccgg cttcttattg atgatgtaag aaagagacag tccgcaatag
     3241 gcaatcacaa cgaggagaca gagattgaaa gtaagagttt gaggccagtg caaggcagcg
     3301 gcaatcaagg ccggtattgt atccagatac cagatcaggg gcggattgac atcgaacata
     3361 tccacgtagg gaatttttcc cctggtgatc agttcagcgc attgcaggtg cagagccgga
     3421 tcccaaccaa ttcgcagtgg atgtgaaaga atagaaacaa gcatgcgcaa gcagagcaca
     3481 aaaacaagga tgagcgaaac tgccgccttc attattgcta ctgcggacgc cagatcttga
     3541 tgactttgcg cgtcgtgtcc acatcgaaac gatagccctt gagcacatag ccgcaaacgg
     3601 taggtctggg caaaagacca tagtcaggcg accatccacg tacgccccga gtctctataa
     3661 cctgaccggg cacatcccgc gcttcaattt tacccatgcg taccgacttc aacttgatat
     3721 tccaggaagt ggtgataata gcctggtttg actccggtcc ctcgctcgac tcagtgcgga
     3781 cattaccgtt gtcgttcaag taggccgagt caacttgcct gatcatatcg gctgagaaac
     3841 ggcaggcaga atcttgaaac tcaaaatatg cttccatagg ctggttatcg aagtaaactt
     3901 tcacccgata gcggctgttt tcaataacat agggaacttc cgtccactct tccttcttca
     3961 tcttattgag gcccgagttg cttctctggg cggtttcgag ggctcgcatc tcctgcctga
     4021 atgatgggtc catctgttcg agagctaccc gcgacaggaa gccggcctcg ctattgctaa
     4081 agcgcttgaa tactttgcga taagtgctct ttgcttcttc tatatagccg gccttctgca
     4141 gacaattggc caggcaatat aaagcccttg gatcctgggg tttgtttgcc acagccgagc
     4201 gcaaaagagg caaggcctcc cggaacttgt ttttcttgta taaggccatg ccggcgccga
     4261 aaggatcgcc tgctccggcg attctctcaa aagtgacttc ccctttttct ttgttgaaag
     4321 ccactttgta accattcaga aaatcagccc ccagaacggc agcatcttta tctttgttgg
     4381 aaaagtagag aggcaattgc gggcgcttga taccgcccac agtgacatcg aagcaagagt
     4441 aaacaccgtc ctcggcgctc ttaccggggc gcccttcaag ggcgattaca ttgagtctgg
     4501 ccttgacggc cacattggag cccaggagcg attcctgccg ctcagtgtcc actatcatct
     4561 taatatcact gccgcccacc ttcacaataa cggtgggctt gccgtcaatt tcctctgctt
     4621 tggctacggt gctcttgggc aaaacatcct gagaaacctg catgttctgc aagccggaac
     4681 ctctggcaaa agtagtattg ggcgagcctg tatttatgga aagagctttg cgggccagtt
     4741 cggccccttc cgagtctgga aagcgattga tcaaggtacc gtaaagttgc ctggccgctc
     4801 ccacctgtcc catgcgatgg tggcagaggg cctggtaata gacgggtcgg aagtcatagg
     4861 cagactcttt ctccgccgcc agaaaatagt tgatggcccc cttgtagtct ttggcattga
     4921 aacaacgcag accgctctga aaggttagat cattatcctg ggcagtggca ggggatccaa
     4981 gcatcaaaag cggcattaaa gccagtgcga gagtgaatct acgcctaaag aaacggtgac
     5041 cttttttcat gtgcatgaac taaaaacctt aactgcagcc atcagccatt attgtgcgaa
     5101 gtaagcaact acctggacgt accgaaaatg cgg
//
LOCUS       DAOQNZ010000073         5092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6758_length_5092_cov_3.066508, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000073 DAOQNZ010000000
VERSION     DAOQNZ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6758_length_5092_cov_3.066508"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="PKW73_02125"
     CDS             <1..1443
                     /locus_tag="PKW73_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72107.1"
                     /translation="QMASAILSGQVPYRDFFDFNPPLIMYLSVIPVVVAKLLPWPVPL
                     CFNFSVLALSIFDTVLLSYLVLRNQTVFPLHQFLPVLFVFAAYGSFVIFDMGQREHLF
                     VLLFLPYCFMRLLSHFQEGVGTCPASLRVLSGLMGGLALSLKPQFFLFGVALEAGLCL
                     ESKRLPWRTWELKALTAVIVVYLLAVLCLPGEALSVYFREALPVYLLGASWNSRCLLH
                     MLLSFDYFREPICLALSTLLLSLVSRPGRYWAVFVLLTLVGFFHYLSGGQAWTYRLIP
                     LQFFSTVLLVYLLGCFVHSFFTRHQAAYRLFLQSAPLLPVATLFFCAFRIFSFYNECA
                     GSPSYDLAPLGYAMVNTRQDFDSAFFSMVSNSLPQEKVVSIGTAISPGFPAQLQALRP
                     PGSRYYYSILVMLKEARERHPRYAPNLLALEAKVVAGLGQDIREQKPRLVYIQTEPLA
                     SILAPYKFNQYLVDYEPAGYVEGSQVYRRK"
     gene            complement(1591..3516)
                     /locus_tag="PKW73_02130"
     CDS             complement(1591..3516)
                     /locus_tag="PKW73_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72108.1"
                     /translation="MNNADNASTPLKTATFDNIWITKVAPFFLLVSLAALVLHRTVFW
                     LMHPLLVSADQSVYLAMAELILKGKVPYRDFFDFNPPLIMYLNTIPISVAHLFHIPTP
                     LGLSLTVSFIHAVASGYIASLLFRFRQYLPAAAMLPVLFVFAAFTTFLDQDMGQREHL
                     FTLLFFPFFIVRGLRHFGAPVGKRERILWSLLCGTILCLKPHFVFIYLMAEAGLWLES
                     RNTSCFFNIEILTMCIPALVYAICFMFLPQEARSLFFDQILPVYIWGNGWAAKCFTHM
                     LVGYDYFAVPFYQATAALIIALVMRNKSRWIPAFLFITIAAMLCYIQGGQAWTYRLLP
                     MAFFAKLLLAFEVGLLGAAVYHKAILLWTGFRLVAAASLLCLTLHYCLGRDLEFLEEY
                     RSAPKFDLTSVGFEGQTPRSDLDVTFFSMVENSNLGDGVVHIGSGIHPGYPCQLQSCR
                     KPASRYLYFMITLIHAAMEKKPELMEKWRDTERIVVENLGNDIIKNRPILVYLQDYPV
                     ADALEKFHFSERYLKNYSLIGQVDVTKIYKRTGSRIDMSAFTPKKRAEIVLPLLCEVK
                     TIEQVSRETTLPPEVLIDWTARAKKAMVDKLKDRGTDREDELRTEITRLSDELWKKSM
                     EADRLRERVKKLENFTE"
     gene            complement(3503..>5092)
                     /locus_tag="PKW73_02135"
     CDS             complement(3503..>5092)
                     /locus_tag="PKW73_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72109.1"
                     /translation="LVALRLSLAQDLMQGKELYTDFFEWTQPFVFEWGKLVYLMQGWL
                     GSLAALRIETISKICFAFALLLSSFLSLIIICQGRPQVRASTDDAQPADNLEFDLVGL
                     AFIASLFIFSLFMRLQLGELQWYLSLAIAPWILLRSYRYSKDNFNCPLILSFFIGILN
                     GFAAILDLPYLVIYFLLETYFLFTFGKFKRNFKSVEMAGFCSGILSTIAHFFCLPTAV
                     RQGYIEWAVPFKLLSWQTFDETLYGPATSPDQTNIYYLAGLAVLAAVWFGRRNAVMIP
                     MALIVLCGSSLNLLEKQGFTRDLILLTYGALSILLMAATSATTASIEFLLAKGQKLSA
                     LLRRTIHFSGYALFVLILSGSSIYLSKRSDKAFGESLTPHPKKVLKGTLDINVAVSQN
                     SQWKDPVIVMCDYPDASYPLLFNLERPNAGYFLTGRPVRLFAALRKLNTLKDNYLDLY
                     DHGRDVLQHRFQTARAKLILLHGAYQLDFINETELRPTLEDKYAREQDVSFYSDNIEP
                     REYLGSDYAFEQYLRRSGEQR"
BASE COUNT         1216 a         1222 c         1272 g         1382 t
ORIGIN      
        1 cagatggcct cggcaattct ttccgggcaa gtgccttacc gcgatttttt cgactttaac
       61 ccgcccctga ttatgtatct gagtgtgata ccggtagtag tggcgaagct cttgccctgg
      121 ccggtgccgc tttgtttcaa tttctcggtt ttggcactta gtatcttcga tacggttttg
      181 ctttcctatc tggttttgcg aaaccagacc gtcttcccct tgcatcagtt tttgccagta
      241 ttgttcgtct ttgccgctta cggcagcttt gttatcttcg acatggggca acgggagcat
      301 ctcttcgtcc tactgttttt gccctattgt tttatgcggt tgctttctca ttttcaggaa
      361 ggtgtaggaa cttgtcctgc ctcgctcaga gttctgtcgg gtctcatggg tgggctggcg
      421 ctgtcgttga agccgcagtt ttttctcttt ggagtggcgc tggaagccgg tttgtgcttg
      481 gagagcaaga ggcttccctg gcgaacctgg gaactcaagg ctttgaccgc cgttatcgtt
      541 gtctatcttt tggctgttct ttgtttgcct ggggaggctc tcagtgttta cttccgggaa
      601 gcgctgccgg tttatttgct gggtgccagt tggaacagcc gttgtttact gcacatgctt
      661 ttgtcttttg actactttag agagccgatt tgtctggcgc tgagcacttt gcttctttct
      721 ctggtcagtc gccccgggcg ctactgggcg gtgtttgtac tgcttactct ggtcggtttc
      781 ttccattatt tgagtggtgg gcaggcctgg acatacaggc ttataccttt gcagtttttc
      841 tcgaccgtat tgcttgtcta tttgcttggc tgttttgttc attcgttttt taccaggcat
      901 caggccgctt accggctttt cctgcagtca gcgccacttt tgcccgtggc gacccttttc
      961 ttctgtgcct ttcgcatttt ttcgttttac aacgaatgcg ccggctcgcc gagctacgat
     1021 ctggcgcccc tcggctatgc catggttaat acccgtcaag acttcgattc agccttcttt
     1081 tccatggttt caaacagtct gccccaggag aaggtggtga gcatcggaac cgccatcagt
     1141 cctgggttcc ctgcgcagtt gcaagctctc agaccgcctg gtagtcgtta ttattacagc
     1201 attctggtaa tgctcaagga agcgcgcgag agacatccac gctatgcccc taatctgctt
     1261 gcccttgaag cgaaggtggt ggccggcctc ggccaggata ttcgcgagca gaaaccccga
     1321 ctggtgtaca ttcagactga gcccctggcc tccattcttg ccccctacaa gttcaaccaa
     1381 tacctcgtag attatgagcc ggccggttat gtggagggtt ctcaggtcta ccggcgcaag
     1441 tagtttgagc ttgcattctc gctgaaaatc ttgttcagtg caggcgctac tcatctttgc
     1501 tccaagtcta tagcggcaac gtcgctttac tggcgagacg tgggttgcca ttaaacttag
     1561 atggtgacct gtttccggct ggcgctccag tcattcggtg aaattttcca gtttcttgac
     1621 tctttctctc aacctatctg cttccatgga ttttttccac agttcatcgc taagccgagt
     1681 gatctccgtc ctaagctcgt cttcgcggtc ggtaccacgg tctttgagtt tgtcgaccat
     1741 tgctttcttt gctcttgccg tccagtctat aagcacctca ggcggtaagg tggtttctct
     1801 actgacttgc tcgatcgttt tgacttcgca caaaagtggc agtacgattt ctgcacgctt
     1861 cttcggtgta aaggctgaca tatcgatacg ggagcccgtc cttttgtaga tcttagtcac
     1921 atccacctgc ccaatcaggc tgtagttttt caggtaccgc tctgaaaaat ggaatttttc
     1981 aagcgcatct gccaccggat aatcttgcaa ataaaccagg atcggcctgt ttttgatgat
     2041 gtcattgcct agattttcta cgacaattct ttctgtatca cgccattttt ccatcagctc
     2101 cggctttttt tccattgctg catgaatcag tgtgatcatg aagtagagat aacgcgaggc
     2161 tggtttccgg caactttgta gttggcaggg atatcctgga tgaatgccgg agcctatgtg
     2221 cactacgcca tcgcccaaat tgctgttctc caccatcgaa aaaaatgtaa cgtctaaatc
     2281 gctgcgtgga gtttgacctt caaaacctac gctggtcaga tcaaattttg gagccgaccg
     2341 gtattcttct aaaaactcta gatcgcgccc gagacagtaa tgtaaagtca ggcacaacag
     2401 gcttgctgct gctacaaggc gaaacccggt ccagagaaga atagctttgt ggtaaactgc
     2461 cgcccccaag agacccactt cgaaggcgag caggagtttg gcaaagaatg ccattggcaa
     2521 aagcctgtaa gtccaggcct gtcctccctg gatgtagcat aacatggcgg ctattgtgat
     2581 aaacagaaag gctggtatcc agcggctttt gttgcgcata actagtgcaa taatgagcgc
     2641 cgcagttgct tgatagaacg ggacggcgaa gtagtcgtaa ccaaccagca tatgggtgaa
     2701 acacttggcg gcccagccgt ttccccatat atatactggc aaaatttgat cgaaaaatag
     2761 acttctggcc tcttgcggca aaaacataaa gcagatggca taaactagtg ccggtatgca
     2821 catagttagg atttcaatat tgaagaagca gctagtgttt ctgctctcca gccatagtcc
     2881 tgcctccgcc atgagataga tgaaaacaaa atggggcttc aagcagagga tggttccgca
     2941 aagtaaggac caaagtatgc gttcccgttt gcctacaggt gcgccaaagt gacgtaagcc
     3001 tcgcacaatg aagaagggga aaaacaagag cgtaaacaaa tgttctcttt gtcccatatc
     3061 ctgatcgagg aaggtggtga aggcggcaaa tacaaaaagt accggcagca ttgcagctgc
     3121 gggcaaatat tgcctgaagc gaaacagcag gctcgcaata tagcctgaag ctactgcgtg
     3181 aatgaacgaa acggtgagag aaagtccgag tggggtgggt atgtgaaata agtgtgccac
     3241 cgaaattggt atcgtattga ggtacataat taggggcgga ttgaaatcga agaaatccct
     3301 gtaaggaacc ttccccttca agattagctc agccatagcc agatatacgg attgatctgc
     3361 gctgactaga agcgggtgca tcagccagaa gactgtgcga tgcaacacta gcgcagcaag
     3421 actgacaagc aaaaagaatg gagcgacctt agtgatccag atattgtcaa atgtggctgt
     3481 ttttaatggc gttgaggcgt tatcagcgtt gttcaccgga cctcctcaaa tactgttcga
     3541 aggcgtagtc gctgcccagg tactcgcggg gctcaatatt atcactatag aaggaaacat
     3601 cttgctctct ggcatatttg tcttccagag tcggtctgag ctcggtctcg ttgataaaat
     3661 ctagttgata ggctccgtgc agcaaaatca gtttggctct tgccgtctgg aagcggtgct
     3721 gcaagacatc tctcccgtgg tcatacaagt ccagataatt atctttcagt gtattgagct
     3781 tgcggagtgc tgcaaacagc cttaccggtc tgcctgtcag gaagtatccg gcattaggtc
     3841 tttccaggtt aaagagcagc gggtatgagg catcgggata atcgcacata actatcacag
     3901 gatctttcca ctgagagttt tggctgacgg ctacatttat gtctagcgta cccttcaaga
     3961 ccttcttggg gtgaggagtc aagctttctc caaaagcttt gtcgctgcgc tttgacaggt
     4021 aaattgagga gccagacaga attagaacga agagtgcata gccactgaaa tgtattgtcc
     4081 tccttagcaa agcgcttagc ttttgtcctt ttgctaggag gaattctata cttgccgtgg
     4141 tggcgcttgt ggcggccatc agcaggatgc ttagcgctcc ataggtaagc agaataaggt
     4201 ctctggtaaa gccttgcttt tccagcaaat ttaatgaaga accgcacaac acaatcagag
     4261 ccatgggaat cattacagca ttgcggcgtc caaaccaaac tgctgccagc acagccagac
     4321 ccgcaaggta gtaaatgttg gtttggtccg ggctggttgc aggaccgtaa agtgtttcat
     4381 caaaggtttg ccagctaagc aatttaaaag gtaccgccca ttcgatgtag ccctgcctta
     4441 ccgcggtcgg taggcagaag aaatgtgcaa tcgttgacag gataccgctg caaaagcctg
     4501 ccatttctac cgacttgaaa ttgcgcttga atttgccgaa ggtgaagaga aaatatgttt
     4561 caagcaaaaa atagatcacc aggtacggca gatcaagaat tgcggcaaat ccattcagga
     4621 taccgatgaa aaaagacaaa atcagggggc aattaaagtt gtctttgcta tagcgatatg
     4681 acctcaacaa tatccaagga gcaatcgcca gagagagata ccattgcaat tcgccaagct
     4741 gcaatctcat aaagagggaa aagataaaga ggctggcaat aaaggcaagt ccgactagat
     4801 caaactcaag attatcggca ggctgtgcgt catcagtact tgctcgcacc tgtggtcgcc
     4861 cctggcaaat aattatgagg ctgagaaagg atgagagcag gagggcaaaa gcaaagcaaa
     4921 tcttgcttat tgtttcaatg cgcaatgccg ctagcgagcc caaccagcct tgcatgaggt
     4981 agaccagttt gccccattcg aagacgaaag gttgagtcca ttcaaagaaa tccgtgtaga
     5041 gctccttccc ctgcataagg tcctgagcca aactaagccg caatgccacc ag
//
LOCUS       DAOQNZ010000074         5087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6772_length_5087_cov_4.551669, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000074 DAOQNZ010000000
VERSION     DAOQNZ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6772_length_5087_cov_4.551669"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PKW73_02140"
     CDS             <1..1191
                     /locus_tag="PKW73_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNA72110.1"
                     /translation="GDVIHCLPLVARLKENLPDCQLHWLVEPAAAPLLFNNPLVDKVF
                     VLPKKEFLAGFKSRKPSAVLASLSTMAHFIKALKRESYDLAIDAQGLFKSAALARLSG
                     AKKVVGFKGTRESAEHWLTDSLDVGDYFAPDCHVVDLNLALADFALRTTGGAKPPSDN
                     LPRDHTLVCFPLPDVTESERGKIEKLLGSFASSSPAVQPLPKPSSADIIQPADVAPYM
                     ATKEKPLIVLIPGTTWDTKIWPAQSWIELACRFQKELDAGIILVGGPSERAANLHIYK
                     GIEAAAGRPHLLADITGETNLIDLLALFERTTLVIGADTGPMHLACAAAAGGGPAVLA
                     VHGASPWGRNGPYGPAGRVAACHLELACQPCFSKHCRLGTIECMKDLPVGKVFQMAIS
                     LLAL"
     gene            complement(1198..1656)
                     /gene="ndk"
                     /locus_tag="PKW73_02145"
     CDS             complement(1198..1656)
                     /gene="ndk"
                     /locus_tag="PKW73_02145"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNA72111.1"
                     /translation="MSQERTFVAVKPDGVERGYVGEIISRFERRGLKLVGLKQMQVSV
                     ELAERHYGEHKGKPFFAGLVGFITSGPIVAMVWEGKGAITLARNVIGATNPAQSAPGT
                     IRGDLAVEIGRNMVHGSDGPESAAREIGLFFKEDELIKDWNRAVDKWVSE"
     gene            complement(1770..4502)
                     /locus_tag="PKW73_02150"
     CDS             complement(1770..4502)
                     /locus_tag="PKW73_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNA72112.1"
                     /translation="MEARSDYGSGGGAGDELLNRLNAEQAQAVTQLWGPSLVIAGAGS
                     GKTTVLTRRIAWLISKLNQEPESILAVTFTNKAAAEMRQRIESIVGPDIARRITIGTF
                     HSICARLLRLEIANYKTTDGLVWDKNFVIYDETDSMSLVKAQINKLNLDDKVFVPKEI
                     RHTISALKNDGYTHEVYAKEAKNYRESRLSEIFSAYQADLARNNALDFDDLILMFTDL
                     MQQNQQVRERTQRRFRHVQVDEFQDTNKSQYDLISLISRVDSPEHFIKRDRPQLSSEE
                     MWYERSLMVVGDVDQSIYSWRKADFRIILGLQKDYEGCRMIKLEENYRSTATILEAAN
                     SIIANNTERIDKVLRCNRGKGGKINCYEAQDEIDEAFYIAEELKRTRARGRKLSDCVI
                     LYRTNSQSRAIEEVLVRSHIPYVVVGGTRFYDRQEIKDMLGYLKLVYNHKDGQAFNRV
                     VNVPKRGLGKTTLDRVEAYAELKGMSAIEACLEAERISELSQKTVFTLKTFAQQVISW
                     QEASQTVSVSALLEKILTDTRYLDKLEEEAHQAKDEVALGRIENIRELVVVAKEFETI
                     ADEPDLDSFLTRISLVSDLDAVKNNEDTVKLMTIHSSKGLEFPSAFIMGLEEGLFPHV
                     RSLDNPAQMEEERRLMYVAVTRAGDALHITLARKRSMVGRGFSGGFSSTFTIPSRFLK
                     EIQPGLISGYYPTGEDGYAKHLERQAGNWGKEPQSRSAGGYERERIPEERSSFDNEQN
                     RKRDRGEYSGFESKYGNNNYSSGGGQRSYGNNSGSTGQYGSGYGARPAANSNSGYGQS
                     KGNFKPGGNSAGQPGRSSYSGGSPYGGQGKSQPPAKPRAMRRPEEVLEVSKEVSYQKL
                     SMGDRVTHVKFGEGTVVQVIGEDDKELYNVEFAEHGKKLLDPKFAKLVKVDPKI"
     gene            4867..>5087
                     /locus_tag="PKW73_02155"
     CDS             4867..>5087
                     /locus_tag="PKW73_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72113.1"
                     /translation="MPPLEPTAATIPTVDELAQELLLIQQSGPRKVAIIGTRNLPLTH
                     QQLVEMLSYALVLSGNQVVTSGGANGTNMA"
BASE COUNT         1014 a         1390 c         1379 g         1304 t
ORIGIN      
        1 ggtgatgtca ttcattgctt gcctctggtg gcccgtttga aggagaacct tcccgattgc
       61 caactgcact ggctggtgga gcctgctgcc gcaccacttc tattcaataa tcctctggtc
      121 gataaagttt ttgtcctccc caaaaaggaa ttcctggccg gcttcaagag ccgaaaacca
      181 tcggcggtgc tagcttccct ctctaccatg gcgcacttca taaaggcgct caagcgggaa
      241 tcctacgacc tggccatcga cgcccagggg ctctttaaga gtgcggccct ggcccgtctt
      301 tccggtgcca aaaaggtggt cggcttcaaa ggcaccaggg aatcggccga acactggctc
      361 accgatagcc tggacgtggg tgattatttt gcccctgact gccatgtagt cgaccttaac
      421 ctggctctcg ccgacttcgc cctgcgcact accggaggtg caaagccgcc ctccgacaac
      481 ctccctcgag accatacatt ggtctgtttc ccgttgcctg acgtaaccga gagtgagcgg
      541 ggcaaaattg agaaattgct tggctctttc gcctcttcat caccagcggt gcagccgctg
      601 ccgaagccat cttccgctga catcattcag ccggcagatg tggcaccata tatggcgact
      661 aaagaaaagc cgctgatagt cttaattccc ggtaccacat gggacaccaa aatttggccg
      721 gctcagtcat ggattgagtt ggcatgccgt tttcagaagg agcttgatgc tggaattata
      781 ctggtgggcg gaccctccga gcgagctgcc aacctacata tatataaagg aatagaagcg
      841 gcggccggcc gtccgcactt actggctgat attaccggtg aaaccaatct catagattta
      901 ctggccctct ttgagcgaac cacgctggtg attggtgccg ataccggccc tatgcacctg
      961 gcctgcgccg ctgccgccgg gggcggaccg gcagtgctgg ccgtccatgg tgcctcaccc
     1021 tgggggcgca acggacctta cggaccggcc ggaagggtgg ctgcctgtca cctggaactc
     1081 gcctgccagc cctgcttttc caagcactgc cgactgggca ccatagaatg catgaaagac
     1141 ctccccgtcg ggaaggtctt tcaaatggct atttcgcttc tcgcactcta gttgcgatta
     1201 ttctgagacc catttgtcca cagcgcggtt ccagtccttg atcagctcat cttctttgaa
     1261 gaagagaccg atttctctgg cggcgctttc cggtccgtcg gaaccgtgca ccatgttgcg
     1321 accgatttcc acggccaggt cacctctgat ggtgccgggg gcggattgag ccggattggt
     1381 ggcaccaatg acgtttctgg caagagtgat ggctcctttg ccttcccaaa ccatggccac
     1441 aatcggaccg gaggtgataa agcccaccag accggcgaag aagggcttgc ctttatgctc
     1501 gccgtaatga cgctcggcca gttccactga aacttgcatc tgcttcagcc cgaccagctt
     1561 gagtcctctt ctttcgaagc gtgaaattat ttcgccgacg taaccgcgtt caacgccgtc
     1621 tggcttaaca gccacaaagg ttctctcttg agacaccgct atttccccct gacaataaga
     1681 gtacgggctt gaccagcccg gcaatatatt atccggctca cccgccgggg aaattggttc
     1741 tgcctcataa ggcgcctaaa gcttaacgtc tatatctttg gatctacttt cactaatttg
     1801 gcgaacttgg gatcgaggag ttttttgccg tgctcagcga actctacgtt gtagagttct
     1861 ttgtcatcct caccgatgac ctgcaccacc gttccctcac cgaacttgac atgggtgacc
     1921 cggtcgccca tggacaactt ctgataggag acctctttgc ttacttccaa cacttcttcc
     1981 ggtcggcgca tggctctcgg tttggccggc ggctggctct taccctgtcc gccatagggg
     2041 ctgccgccgc tgtatgaact tctgccgggc tgaccggcgg agttgccgcc tggtttgaag
     2101 ttccctttgc tctgtccgta accggaattg gagtttgccg caggccttgc accatatcct
     2161 gaaccatact ggccggtgga acccgaatta ttgccgtagc ttctttgccc gccgccgctt
     2221 gagtagttgt tgttgccgta cttgctttcg aagccggagt actcgccgcg atcccgtttg
     2281 cgattttgct cgttgtcaaa tgagctgcgt tcttccggaa ttcgctctct ttcatagccg
     2341 ccggcagatc tgctttgagg ttctttgccc cagttgccgg cctgcctttc caggtgcttg
     2401 gcgtagccgt cttcaccggt gggatagtag ccggagatga gaccaggctg aatctccttg
     2461 aggaagcggc tgggaatggt gaatgtagat gagaagccgc cgctgaagcc gcgacccacc
     2521 atggagcgtt ttctggccag ggtgatatgc aacgcgtcac cggctctggt gacggcaaca
     2581 tacatgagcc ggcgctcttc ttccatctgg gccggattat ccagggagcg cacatggggg
     2641 aagagacctt cctcgagacc catgatgaag gcggagggga attccagacc tttggaagag
     2701 tgaatggtca tgagcttgac ggtgtcttcg ttgttcttga cggcatcgag gtcgctcacc
     2761 agggagatgc gggtgaggaa ggagtcgaga tctggctcat cggcgatggt ctcgaattct
     2821 tttgccacta ctaccagttc tctgatgttt tcgatgcggc ccagtgccac ttcatcttta
     2881 gcctgatggg cttcctcttc cagtttgtcc aggtagcggg tgtcggtgag aattttctcc
     2941 agaagggccg aaacggatac tgtctgagag gcttcttgcc agctaattac ttgctgggca
     3001 aaggtcttca aagtaaaaac ggttttctgg gaaagttcgg aaatgcgctc tgcctccagg
     3061 caagcttcga tggcgctcat ccccttcagt tcggcatagg cttcgactcg atccagtgtg
     3121 gtcttcccga gacctcgttt cggtacattc acgacccggt tgaaggcctg cccgtcctta
     3181 tggttgtaga ccaacttcag gtagccgagc atgtctttga tttcctggcg gtcatagaag
     3241 cgggtgccgc ctacaaccac atagggaata tggcttcgca ccagcacttc ttcaatggcc
     3301 cgcgattgag aattggtgcg gtagagaatc acgcagtcgg aaagcttgcg gcccctggcg
     3361 cgggtgcgct tcaactcttc ggcgatgtag aaggcttcat cgatttcgtc ttgtgcttcg
     3421 tagcagttta ttttgccgcc cttgccgcgg ttgcaacgca agactttgtc gattctctcg
     3481 gtgttattgg caatgatgct gttggctgct tccagaatgg tggccgtaga tctgtagttc
     3541 tcttccagtt tgatcatgcg gcagccttca tagtcttttt gcaggcccag gatgatacgg
     3601 aagtcggctt tgcgccagga atagatggat tggtctacat ctccgaccac catcaagctg
     3661 cgttcgtacc acatctcttc ggagctgagc tgggggcgat cgcgtttgat gaagtgctcg
     3721 gggctatcca ctctggatat caacgaaatc aaatcgtact gtgacttgtt ggtgtcctga
     3781 aactcgtcta cctgtacgtg gcgaaaacgc ctctgggtgc gctccctcac ttgctggttt
     3841 tgttgcatga gatcggtgaa catcaaaatc agatcgtcga aatcgagagc gttattgcga
     3901 gcaaggtcgg cttgataggc ggagaaaatt tcggaaagcc ggctctctct gtagttcttc
     3961 gcttctttgg cgtagacttc gtgggtgtaa ccgtcattct tgagggcgga aattgtatgc
     4021 ctgatttctt tcggtacgaa aactttgtcg tctaaattga gtttgttgat ctgggctttt
     4081 accaggctca tactgtcggt ttcgtcgtag atgacgaaat tcttgtccca gaccagaccg
     4141 tcggtagttt tgtagttggc tatctcgagg cgaagcaggc gggcgcaaat gctgtggaaa
     4201 gtgccgatgg ttatgcgtct ggcaatatcc ggtccgacga tggactcgat gcgctgccgc
     4261 atttcagccg ctgccttatt ggtgaaggtg acggccagaa tagattccgg ttcttgattg
     4321 agttttgaaa tcagccaggc gatgcggcga gtcaagaccg tggtcttgcc gctgccggca
     4381 ccggcaatca ccagcgaagg tccccagagc tgggtgacgg cctgtgcctg ttcggcattg
     4441 agacgattga gcagttcatc gcctgctcct ccgccgctgc cgtaatcgga tctcgcttcc
     4501 attacttggt tttccgccgg tatctctata gccactgcat gtctccgcta agtctctgct
     4561 ctggggaaag tgatgtcaag caaagccgcc cttttagggt ggatatctct ttgctatgcg
     4621 atgcgctctc tttggaaatc gcccgggatt gggacagagg ctgccatcta ctctattgtc
     4681 ctcaataagg ggggggatcc gttaattaaa acttgtaaac agcaaagctc ttgcttttgc
     4741 caaaggcctc ggaagacttg taatcttaag gttgccagag ttgagatcat gttcaaaccc
     4801 taaggtaaaa acttttgcct tcagttttcg actcagcatc aatatatagg gatcgagagg
     4861 aagtatatgc cgccactaga accgactgcc gccactatcc cgactgtaga tgagctagcc
     4921 caggagttgc tgttgattca gcagtccggt cctcgcaaag tggcaatcat cggtactcgc
     4981 aacttgcccc tcacccacca gcaactggta gagatgctct cttatgccct ggtgctttcg
     5041 ggcaaccagg tggtcaccag cggtggcgcc aacggcacca acatggc
//
LOCUS       DAOQNZ010000075         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6774_length_5086_cov_4.645796, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000075 DAOQNZ010000000
VERSION     DAOQNZ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6774_length_5086_cov_4.645796"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2187)
                     /locus_tag="PKW73_02160"
     CDS             complement(<1..2187)
                     /locus_tag="PKW73_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNA72114.1"
                     /translation="MSNTSNSPSPLTGAADAIIAASSSTATKPLAGNTSPAVEQTIKD
                     NPGTEDRLGYNYEGAKQFPNDIKKRTIPPKARKQYPPAAPKVAAFATNTVQGPASIVE
                     LSRALNVENNGPQLMYEWVYSNIEWEPGWGLNKGPVGSIADGLGNQFDQSALLAALLR
                     QAGFTAHIVMGTVRLNEAQFNAWFGTDSIWAARNYCFNLFIPVVTEPTWDGTTYYMDI
                     KHVWVRWIDGANTWNFDPSVKSYTRKTGRTDLATILGYNAATFMSNAQSGATVTTDYA
                     QNMNRANVRNDLTTFSTNLANWIKANDPDAQVDDLIGGQSINAPTLPVLQSTLPYQAP
                     GDTPTVWTGDVPASFKPTLQVQFPNWTTPGVWDIDWQTTSDQLANSRLSLFYDGSLVP
                     SLYLNGTAVDTGLAQPPGTYTSILLTVTHPAYDGANYPLSYQRYYQTTYQWWQGGIYA
                     GDSFLIANAWGNLGQGQLDYHQTEVDKALAAGGADTSEPVLGSRLAMTWYRMASASSR
                     ICELVNQIKKCRTVYSHQVGVMSFKNTLAGEFGADIGGVSGSSTNLNNDMTQIPSNDT
                     TISMHGVAFEALTLSQVAGTSPGISATTIVDKAIQLGQKIYKGTTSNWNTGSNIEAAL
                     VANGYSSTDMANLYNWYIQWGNDLVIGENPAVTLDTWTGWAYWVYPTSGAFGIINGAF
                     KGGRKKGKPTKKVPKKGKDAKPIGDPISAFDGSMILDTDDLDIGSMD"
     gene            complement(3185..3988)
                     /locus_tag="PKW73_02165"
     CDS             complement(3185..3988)
                     /locus_tag="PKW73_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72115.1"
                     /translation="MGKLIDQDDEDIVNPIDRGAKEEDKVKELRRLSLENEPKVKEFL
                     EKLDSKYGTESKTSFKEPEKILEKASRPSIRADKPWHDVEHVRDSFRFKTVLENIEQL
                     PKIAEDLKEAGFTVIKTDTEKVLRPGFWGWRIAALDLKMPNGQLVEYYLPLKELEEAK
                     KNGNHELFEKWRNEDPETLSKEEQRQYLSDSYDSYDKYEAAWQTYLERTKQKPEHVKA
                     ALEATEKVFEKDRAKTEEKNVIKKSESKLIQKIKEQKAKEKAKEDDLLR"
     gene            4069..4287
                     /locus_tag="PKW73_02170"
     CDS             4069..4287
                     /locus_tag="PKW73_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72116.1"
                     /translation="MSYRVHKSITGNVVVASREDDFIASFKDGSWLDPLAFNAHELED
                     MLLVTDRSEAESLIKQAKNALSHDAIVA"
     gene            complement(4347..4790)
                     /locus_tag="PKW73_02175"
     CDS             complement(4347..4790)
                     /locus_tag="PKW73_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72117.1"
                     /translation="MLEASAEIKEKLQEMAHEASRPFCYSDYVTVEADENGQYRCPKC
                     GSDDLMREVEGVGVEWGYDWVMEHLVETQGERVDIEELYRDLLDDIYEPVRFGELEYS
                     PSAVLEAVDPVAFRIGAQENADSAVEDSLMVCLNGTYYRISDIAE"
BASE COUNT         1159 a         1320 c         1276 g         1331 t
ORIGIN      
        1 gtccatgctg ccaatgtcga ggtcatccgt gtcaagaatc atggaaccat cgaaggcact
       61 gataggatcg ccgataggct ttgcatcttt ccccttcttt ggaaccttct tagtaggctt
      121 gcctttcttt cgaccgcctt tgaaggcacc gttgatgatc ccgaaagcgc cagaagtggg
      181 atagacccaa taagcccagc cagtccaggt gtcaagggtt acagccggat tttcaccgat
      241 gaccaaatcg ttcccccact ggatgtacca gttatagaga ttggccatgt cggtagagct
      301 gtagccattg gcgacaagtg cagcctcgat gttggaaccg gtattccagt tggaggttgt
      361 tcccttgtag atcttctgac caagctggat agctttgtca acgatggtcg tcgcactgat
      421 gccaggggaa gtccctgcca cctgcgatag agtcaaagct tcaaacgcaa caccgtgcat
      481 ggagatggtg gtgtcgttgc tgggaatctg ggtcatgtca ttgttgaggt tcgtgctaga
      541 gccagacacg ccgccaatat cagcaccgaa ttcaccagct aaagtgttct tgaaggacat
      601 gacaccaacc tgatggctgt atactgtccg gcactttttg atctggttga ccaattcgca
      661 gatacgggaa gaagcagaag ccatgcggta ccaagtcatt gcaagacgtg agccaagcac
      721 gggctccgaa gtatcggcgc cgccagcagc aagagcttta tcaacctcag tctgatgata
      781 gtccagctgc ccttggccaa ggttgcccca ggcattagca ataaggaagg aatcaccggc
      841 atagatgccg ccctgccacc actggtaagt ggtctggtag tagcgctgat agctcagagg
      901 gtagttcgcg ccgtcatagg cgggatgggt taccgtcagc aggatggaag tataggtacc
      961 gggcggctgc gccaaaccgg tatcaacggc ggtgccgttc agataaaggc tcggaaccag
     1021 acttccatca tagaagaggg agagccggga attggcgagc tggtcggaag tggtttgcca
     1081 gtcgatgtcc caaacaccag gagtagtcca gttcgggaac tgaacctgaa gggttggctt
     1141 gaaggaagcg ggaacatcgc cggtccaaac ggtcggggta tcaccagggg cctggtaagg
     1201 cagagtcgat tgaagaaccg gcaaggttgg ggcattgatg gactgaccac caatcaggtc
     1261 atcgacctgg gcgtcagggt cattggcctt aatccagtta gccaggttgg tggagaatgt
     1321 ggtcagatca ttgcgaacgt tggccctgtt catgttctga gcatagtcag tggtcaccgt
     1381 ggcccccgac tgagcattgc tcatgaacgt cgcggcattg taaccaagga tggtggccaa
     1441 atcagtccgg ccggtctttc tggtgtagga cttgacgcta gggtcgaagt tccatgtgtt
     1501 ggcaccgtca atccagcgga cccagacatg cttgatgtcc atgtagtagg tagtgccgtc
     1561 ccaagtgggc tcggtgacaa ccggaataaa gaggttgaag cagtagttcc gggcagccca
     1621 gatactgtca gtaccaaacc aagcattaaa ctgagcctcg tttaatctga cggtccccat
     1681 gacaatatgg gcggtaaagc cggcctgacg gagaagcgca gccagaagag cgctctggtc
     1741 gaattggttc ccaagaccat cggcaatcga gccaactggt cctttattaa gtccccaacc
     1801 gggttcccac tcaatattgg aatagaccca ctcatacatg agctgaggtc cgttgttttc
     1861 tacattgagt gcgcgggaaa gctcaacgat agaagcaggg ccctggaccg tgttggtggc
     1921 aaaggcggca accttaggag cagcaggcgg gtactgcttg cgagccttcg gaggaatagt
     1981 gcgcttcttg atgtcattgg ggaactgctt ggctccttca tagttgtagc caaggcggtc
     2041 ttcagtgccg ggattgtcct tgatggtttg ctcaacagcc ggggaggtat taccagcaag
     2101 cggcttggtt gcagtggaac tggaggcagc gatgatggca tctgccgctc cggtcaacgg
     2161 ggaaggtgag tttgaggtat tagacatatt gtcttttcct tgattttgtt tggtttacgg
     2221 acgaacttgc acaggctcag agaaacaacc tgtgcgacaa gcagacaaac agagggtttg
     2281 cctgctatgc ttcgttgtca ggcgctccat tcaacatgtc ccagatgggc atgaggtaac
     2341 aacagaagtc ctgtttcctg actggtcgta attgaacgta atgactgtac cgttagtgta
     2401 ggtaatggtt ttgagacgac caaggttgtc ataggtgtag gttgccgtat ctgcggccat
     2461 agtctttatc ctttgtgtga gtggatttca tcgattcgga atagtccaaa tcgacacgtt
     2521 gaacagcagc aactgttcac ttccttttga taaggagcga cttttgctgc ggcgattatt
     2581 tagattagag accttagaga atttagctgc gctagccaag gagccccagc gctgccttct
     2641 atgaggatct ctttcatctg gtcaacttca ttacatagcc caacccgacc atatgcctgg
     2701 ctctgaggaa ttcaattgaa tcggtccggt taggggtggt ttcgaggaaa cgctcgtggt
     2761 aagtgcaaat gaagttgata cgacttttct agcgctgcca gggggccaag ttcaacttcg
     2821 cccggacttg ctgacatctg tcagaggaga cggttgtcac tagaacaaga gactctccaa
     2881 ttcactaaaa gcgtgacaag ccaagatgat tttccttcac tcttgcacaa gaataaggaa
     2941 acgctcacac gaatttcgag tactggcgcg gcttgcgcga cttcccggtt tctaattaaa
     3001 gcgtaatgcg ccttaagatt agcttactga gccatttctc attgtgaacg atcaataagc
     3061 cgattttaca ttacttcaat cagcaagtcg aggacttatt tctagagaat caaccacatt
     3121 tcaagaagct gatctgcccc taccgcccca cctcttgaaa atagtgaacc atatggaaac
     3181 cggactaacg caagaggtcg tcttctttcg ccttctcttt ggccttctgc tctttgatct
     3241 tctgaatgag cttgctctct gacttcttaa tcacattctt ctcttctgtc ttggctctgt
     3301 ctttctcaaa taccttctct gtagcttcca gggccgcctt gacgtgctcc ggcttttgct
     3361 ttgttctctc tagatacgtc tgccaggctg cctcgtactt gtcatagctg tcatatgaat
     3421 cggaaagata ctgtcgttgc tcttcttttg ataatgtctc cgggtcttcg tttcgccact
     3481 tctcaaagag ttcatgattg ccgtttttct tagcctcttc caattctttt agaggcaagt
     3541 aatactcaac aagctgaccg ttaggcattt tcagatccaa tgccgctatc ctccatcccc
     3601 agaagcccgg cctcaaaact ttttctgtgt cggtcttgat cactgtaaag ccagcttctt
     3661 tcaggtcttc tgcaatttta ggcagctgct cgatgttttc taagactgtc ttgaacctga
     3721 acgaatctct gacatgctca acatcatgcc agggcttgtc ggctctaata gaaggtctcg
     3781 atgccttctc cagtatcttc tcaggctcct tgaagctggt cttagactcg gttccgtatt
     3841 tgctatccag cttttccaga aactctttaa ccttgggctc gttctccagg gataagcggc
     3901 gaagctcttt taccttgtct tcttcttttg ctccgcgatc tatcgggttg actatgtcct
     3961 catcatcctg atctatcaac ttccccaaag cacgccacct gcttcaattt acggttgtaa
     4021 agcccaccca aatatgccat aatagagtag cagatagggg tacacgcgat gtcgtatcga
     4081 gtccacaaat caataaccgg caatgtggtt gttgcctcca gggaagatga tttcattgcc
     4141 tctttcaaag atggaagctg gctggatccc cttgctttca acgcccatga acttgaggac
     4201 atgctcctcg tcactgaccg tagcgaagcc gaaagcctca ttaagcaagc caagaacgct
     4261 ctttcccatg acgcgatagt ggcatagctg gcttcgacaa acccagacag atagtcaaaa
     4321 ggcggctctt ggagccgcct ttcttttcac tcagctatgt cgcttattcg gtaataagtc
     4381 ccgttcaggc agaccatcag agagtcttcc acggcgctat cggcattttc ttgcgcccct
     4441 atcctgaaag ccaccggatc aaccgcttcc agcaccgctg acgggctata ctccagctcc
     4501 ccgaacctga ccggctcata gatgtcatct aagagatccc ggtaaagctc ctcaatatcg
     4561 accctctcgc cctgggtctc taccaggtgc tccatcaccc agtcatagcc ccattcgact
     4621 cccacacctt ccacttctct cataaggtca tcactgccgc atttggggca gcggtactgc
     4681 ccgttttcat cggcttctac cgtgacatag tccgagtaac aaaacggcct tgaggcttca
     4741 tgagccatct cttgcaactt ctcttttatc tctgcgctcg cttccagcat aggcacctcc
     4801 aatatttccg gtgcgtcgcc gcacccccat gccgcacatg cgctattgcg aagaccaggg
     4861 tcccagagtc aagaccgccg aaggcgcctt taggctcggt cttgactctg ggggtctgaa
     4921 gttacgctct cttgcgggca tggaaaggaa ggctgacgcc tacctcaagt cgcccgcttt
     4981 gacttttctc ttgcttgctt cttctcgtgg tactctctcc accgctttgc ctgtgacttt
     5041 gcgaattatt tggcagactt tgtggcgaat tatttggcaa aaattg
//
LOCUS       DAOQNZ010000076         5067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6826_length_5067_cov_3.126297, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000076 DAOQNZ010000000
VERSION     DAOQNZ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6826_length_5067_cov_3.126297"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="PKW73_02180"
     CDS             complement(<1..1330)
                     /locus_tag="PKW73_02180"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNA72118.1"
                     /translation="MNENSKDIPLCAQPELVQRPEGQAEAVSLAEPSKNPRLVVEPSE
                     EGTGNTIELTGDTPAPGSVAPADEPPENAAKVAAPGLVQNRASFTRRFLALALDTGVI
                     ASMGSTIAFVGFLLLLQIANATGVEGANLPLGEQIFLSLQGALVFWFTQLMPFFNALT
                     AVLGGGIAHYFTYTLANNVNLNAPGLPQFLAGGILWIILTANCVLSVFYHTLLTASPL
                     KGTLGQRLLNITVVGAGGKGLSIGQSLLRTAIKLVVCVVVSMPLAVLLTRAPLFFVLL
                     LAGITFLDLSKKGRSARSRLEFLLRKACHNFSVLEGGKESFNTLTLSEDENLPAPEKK
                     RLQTHRDHILIRYKPYAAVSRWLEQRFSHDANIAKLDVLLNAEMVKLEAWADANRAAR
                     FTEKKSIEIGGAVFGWHAGNWKTETVKRTTWKEIVARLMSFVTLGKMPKAT"
     gene            complement(1651..4203)
                     /locus_tag="PKW73_02185"
     CDS             complement(1651..4203)
                     /locus_tag="PKW73_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNA72119.1"
                     /translation="MAQNTMSRVDSAPEQEHKSGYQVLENGKVLAPCEGGVCKPPMDE
                     NKFMKDVLVTPENQEAWKQWRQSHPNGSSELFHPVEATGGKMTAGDKAKFPGINLFDS
                     ERFDVPEAVLKAKETGKAGEKVVEPGNIKPQEGVKPSTEQYPSLDEIKRSSLLNLRRN
                     STIQCHDSGQNQKPELDFRSLDSLFKQKPGKDLNSLESLLKQDGAPKPPKPGEEQRLD
                     KNSAQYKELEQFLKGLENQGKKDSPKNQPAENSQTPPADKAVDKPADQPAENSQTPPA
                     DKSVDKPADQPAENSQTPPADKSVDNSVDKQAEKKADLPKLEEKPLSPFQQAIKDSGA
                     YTLDNLQPAMNAAAKDKAGVAFMIVGENSPGSKELLAKLPELQKQHPDLKFVVVNKDE
                     IDKKVNENPKDQEAQSWKGWIDHNLRDCSGKPIDYTFTSVQTLKADAAGKPMPDKVTS
                     THWGADIEAGLADQSRYAAAGTARNADKFKLAMSADDTANAVKALEALKPQGEAAANN
                     PAENKVRQEQFLKSIKQADSMDPELLAKRKEEVQAQLEEAKREQKKLDYESSPSDKPA
                     DTSKLDKLNLQLQQVETLEKAPGQLRAEMGFEMLNQASNEKNPARQEAMKALAGELLR
                     NSIKADPELANNEDFNCRLFAQGIDAAKLAEQAKSSSQVLNATQLAGALERAATAGVI
                     NDSNVKPVSHDQPKPVKNETDLNKQVPQEIEKVGPEKKLEAPAKTVYDGAGYKDALAE
                     AQRRGLPVVFKVGAEWCPPCQNFERNMAGNLENTYKDKAIVVKVDSDRNPDIARALGA
                     NRLPTSGVGYVDESGRLRIQNSFVGFSGNQDWMRRIQSGMTSAQTNINRMPR"
     gene            4465..>5067
                     /locus_tag="PKW73_02190"
     CDS             4465..>5067
                     /locus_tag="PKW73_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72120.1"
                     /translation="MAIESDRRKNPTKDLVGLEMAPLAQMPLGTPSLNIDLLNCDFLL
                     VHEKRSDIEVRANYPGHWHCESTGLVRQTQVARCYPGFYHTSNGNNLLGMGTVPAPLP
                     AERFCINITGEGIYMGGKKMPVERKLSRPDSVGFDMNERGMFLDGHRLKPMNIKGSDL
                     EVDTVKILLPEDFQGCLHLTWKGSLPAKMDFWSGGKCRLLI"
BASE COUNT          931 a         1297 c         1397 g         1442 t
ORIGIN      
        1 ctgtggcctt gggcatcttg ccgagcgtga cgaagctcat cagccgcgcc acgatctcct
       61 tccacgtcgt gcgcttcacg gtctctgtct tccagttccc cgcgtgccag ccaaagaccg
      121 cgccgccgat ctcgatgctc ttcttttccg tgaagcgcgc cgcgcgattc gcatccgccc
      181 acgcctccag cttcaccatc tcggcattca gcagcacatc cagcttcgcg atgttcgcat
      241 catggctgaa gcgttgctcc agccagcggc tcacggcggc atagggtttg tagcgaatca
      301 gaatgtgatc cctgtgggtt tgcagtcgct ttttctcagg agccggcaag ttctcgtctt
      361 ctgagagggt aagggtattg aaggattctt tgcctccttc caggacgcta aagttgtgac
      421 aggcttttct aagcaggaac tccaaccggc ttcgtgccga gcgacccttc tttgataggt
      481 ccagaaaagt gatgcctgcc aaaagcaaaa caaagaaaag cggtgctctc gtaagaagca
      541 cggccagggg catagacacc actacgcaga ccaccagttt gatggccgtc ctcagtaaag
      601 actgacctat tgaaagacct ttaccgccgg ctcccaccac ggttatgttg agcagtctct
      661 gcccaagggt gcctttgagg ggtgatgccg taagaagggt gtgatagaag accgagagta
      721 cgcagttagc ggtgagtatt atccagagga tgccgccagc taaaaactgc ggcagtcccg
      781 gggcattcag attgacattg ttggccagcg tataggtgaa atagtgcgct ataccgcctc
      841 ccagtacggc tgttaaggcg ttgaagaaag gcatcagttg agtaaaccag aagacgaggg
      901 ccccctgcag actgagaaag atttgttcac cgaggggtaa attagcgccc tccacaccgg
      961 tggcgttggc tatctggagc aaaaggagaa agcccacaaa tgcaatggtc gagcccatag
     1021 aggcgataac accggtgtcc agagccaggg ccaggaagcg tctggtaaaa ctggctcgat
     1081 tctgaaccag gccgggggcg gctactttgg ccgcattctc gggaggctca tcggcgggag
     1141 ctactgaccc tggcgcaggg gtgtcgccgg taagctctat ggtattgccg gtcccttcct
     1201 cagaaggctc aaccaccaga cgtggatttt tcgagggctc cgccaggctc accgcttccg
     1261 cctgtccctc cgggcgctgt accagttcgg gttgggcgca cagcggaata tctttcgagt
     1321 tttcgttcat tctctcttta aatggttcga tagccggcag caagtactcg ggattccata
     1381 tcttctgtac cctggggcgg tgcctctaag ccttgccagg aaagttttgt tatataacgc
     1441 agaaagtgtt tttgttccaa aggctcgact gggccgtgat caccctattt actgagattc
     1501 tctcaggagt tcttgcttgc gagtatcggt tgaatggcgg agtttttccg ccgcttccag
     1561 aaaacctttc agggtacatc ggtcaagtga ccgatatacc ctgtgaattt gcgaaatcct
     1621 gaaatttgac cgttatcggt tccggatacc tcagcggggc atgcgattga tattggtctg
     1681 ggcgctcgtc atgccgcttt gaatgcgacg catccagtcc tggttgccgc tgaatcccac
     1741 gaaggagttt tgaatgcgca gtctgccgct ctcatctaca taacccactc cggaggtagg
     1801 cagtctgttg gcacccagtg cgcgggcaat gtcgggattt cgatcggagt ctactttcac
     1861 tacgatggct ttgtctttgt aggtgttttc cagattgcct gccatatttc tttcgaagtt
     1921 ctggcagggt gggcaccatt ccgctccaac cttgaagact accggcagac ctcttctttg
     1981 tgcttctgcc agcgcatctt tgtagccggc accgtcatag actgttttag ccggggcttc
     2041 caatttcttc tccgggccca ctttctcgat ttcttgcgga acctgcttgt tcaggtcggt
     2101 ttcgtttttg acgggttttg gttggtcgtg gctgacgggc ttcacattgc tgtcgttgat
     2161 tacaccggca gtggccgcgc gctccagggc tcctgctagc tgcgttgcgt ttagaacctg
     2221 gctgctgctc ttggcctgct ccgccagttt ggcagcatcg atgccctgcg caaagagtcg
     2281 gcaattgaag tcttcattgt tagccagttc cggatctgct ttgatggaat tccgcaaaag
     2341 ttcgcctgcc agagctttca tcgcttcctg tcgcgcagga tttttttcat tgcttgcctg
     2401 gttgagcatt tcgaaaccca tttccgctct caattgaccg ggggctttct caagtgtttc
     2461 aacttgctgc aactgcagat ttagtttgtc cagcttggat gtatcagccg gtttgtcgct
     2521 gggtgagctt tcgtagtcta atttcttttg ttctcttttg gcttcttcca gttgggcctg
     2581 cacttcctct ttccgcttgg ccagcagttc gggatccata ctgtcggctt gtttgatgga
     2641 ttttagaaat tgctcctggc ggactttgtt ttccgccggg ttgtttgctg ccgcttcccc
     2701 ttgcggtttc agagcttcga gagcttttac ggcattggct gtatcgtcgg cgctcatggc
     2761 cagcttgaac ttgtcggcat tacgggcggt gccggctgct gcatagcgag attggtcggc
     2821 taagcctgct tcgatatcag ccccccagtg cgtgcttgtc actttgtcgg gcattggttt
     2881 gccggctgcg tctgctttca gggtttggac gctggtgaag gtatagtcga ttggtttacc
     2941 gctgcaatcc ctcagattgt gatcgatcca tcctttccaa ctctgtgctt cctggtcttt
     3001 cggattctca ttcaccttct tgtctatttc gtccttattg accacgacaa acttcaagtc
     3061 aggatgttgt ttttgcagtt ccggtaattt tgccagtagt tctttcgagc cggggctgtt
     3121 ttcacccacg atcatgaaag caacgccggc tttgtcttta gctgcggcat tcatggccgg
     3181 ttgcagatta tccagggtgt aagcccctga atctttgatg gcttgctgga acggggaaag
     3241 gggcttctct tccaatttag gcaggtcggc ctttttctca gcctgcttat ccactgaatt
     3301 gtcgactgat ttgtcggcag gcggggtctg cgaattctct gccggttggt cagcgggctt
     3361 gtcgactgat ttgtcggcag gcggggtctg cgaattttca gcaggttggt cagcgggctt
     3421 gtcgactgct ttgtcggcgg gcggagtttg ggaattttcg gcgggttggt ttttggggct
     3481 gtccttttta ccttgattct ctaagccttt gaggaattgc tctagctctt tgtattgagc
     3541 tgaatttttg tcgagccttt gctcttctcc tggcttaggc ggctttggcg ccccgtcttg
     3601 tttgaggaga ctttccagag aattgagatc ctttcctggt ttctgtttga agaggctgtc
     3661 cagagatctg aaatccagtt cgggtttctg gttctgtccg gagtcgtggc actgaatggt
     3721 gctgttgcgt cttaaattga gcaggctgct tcttttgatt tcgtctagcg aagggtattg
     3781 ctctgtggat ggtttcactc cctcttgagg tttgatattg ccaggctcga ccaccttttc
     3841 gccggccttg ccggtttctt ttgccttgag cactgcttca ggcacatcga acctttccga
     3901 gtcgaagagg tttatgccgg gaaacttggc cttgtcaccg gctgtcatct tgccgcctgt
     3961 ggcttcgacc ggatggaaca gttcgctgga gccattgggg tgggactgtc gccactgttt
     4021 ccaggcctcc tggttctccg gtgtgaccag aacatctttc atgaacttgt tttcgtccat
     4081 gggaggtttg cataccccac cttcgcaggg ggccaggact ttgccattct caagcacctg
     4141 gtagcctgat ttgtgctcct gctccggtgc ggaatcaacg cgggacattg tattttgagc
     4201 catggtaatt agttccgtaa gaaagaatgg ggtcggtatg acaaccctat gtgataattg
     4261 tttcattgtt gtttcagggc agtcgtagca cctgttcgca ctgctggcct tcctaaaata
     4321 gggcgggcac cggtaggtct ttttgcccgg gtctccacca tcggttttgg ttcattagta
     4381 tttatgatgc ggcgcagtca ctttcttttg ttgtcagctc tgggagccgc aaccctgctg
     4441 ccttgctttt gggcgccgca ggctatggct atagagtctg accgcaggaa aaatccaact
     4501 aaagacctgg tcggtttaga aatggctcct ctggcgcaaa tgccccttgg tacgccgtct
     4561 ctgaacattg atctgctcaa ctgtgacttc ttgcttgtgc acgagaagcg ttccgatata
     4621 gaagtgcgag ccaactaccc cgggcactgg cattgcgaga gcaccggtct tgtgcgtcag
     4681 acccaagttg cccgctgcta tccgggcttt taccatacca gtaacggcaa taatctatta
     4741 ggaatgggca ctgtaccggc gcccttaccg gcggaacggt tttgcatcaa tatcaccggc
     4801 gagggaattt acatgggtgg taagaagatg cccgtggaaa ggaaactgag ccgacctgac
     4861 tcagtgggct tcgacatgaa cgagcgcgga atgtttctgg atggacatcg gcttaagcct
     4921 atgaacatta agggcagtga ccttgaagtt gatacggtca agattctttt gccggaagat
     4981 tttcagggat gcttgcacct gacctggaag ggatctttgc ccgcgaagat ggatttttgg
     5041 agtgggggta agtgccggct attgatt
//
LOCUS       DAOQNZ010000077         5062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6839_length_5062_cov_3.757340, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000077 DAOQNZ010000000
VERSION     DAOQNZ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6839_length_5062_cov_3.757340"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKW73_02195"
     CDS             <1..500
                     /locus_tag="PKW73_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72121.1"
                     /translation="VEGGAALFLIITGTMLAISFLVSSGMLVYYKQKMGFISTQIAQQ
                     VGMHPQMDDMTMTEMTRDMMAKMSLPTANMSVSRSNTDVYGRPGVAITVKNGKLPLFT
                     NVDFLPMTIDIQDTAVAFLSASPSGADAYLWMPRGRMSSYVLPMVRIPNGGTPQFSTV
                     PVIQY"
     gene            complement(623..1615)
                     /locus_tag="PKW73_02200"
     CDS             complement(623..1615)
                     /locus_tag="PKW73_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNA72122.1"
                     /translation="MAQEPLSISDVLVARKRITGFVTNTPLRKSHWMSDVSQAEVWMK
                     LENLQVTGSFKYRGALNAMTFAKERGITRVFTASAGNHALGMAEAARTTERETTVCLP
                     TNVAAVKKAKLELFSVGIVQHGDDMELTEQYAARLALEKKGLYISPYNNREVIAGQGT
                     VGLEMYELVPKLSTLVVAVGGGGLISGIGLVAKVINPKVRIVGVVPAASPSMRTAVQS
                     GHVVRTLQQETIADGIAGNIDPETITFPLVQEVVDEWVTVEERDIRQAVFDFLEHEGM
                     LIEGAAACAIAAISSKYVEFKPRERVGVVVCGGNIGRSTWREIVGEQLLSAGKA"
     gene            complement(1690..1763)
                     /locus_tag="PKW73_02205"
     tRNA            complement(1690..1763)
                     /locus_tag="PKW73_02205"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1729..1731),aa:Gly,seq:tcc)
     gene            2254..3447
                     /gene="hisC"
                     /locus_tag="PKW73_02210"
     CDS             2254..3447
                     /gene="hisC"
                     /locus_tag="PKW73_02210"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNA72123.1"
                     /translation="MATFIQQLASMSQQLALVSDPKNTIRFNPDIQPELPKRLVPPHV
                     ESLKPYQPGRPPEQLKKELGIERFVNMASNENPLGPPPSALAAIQENLLDINRYPDLC
                     GTKLREALAERYATKVDNIALGSGSESTMANIIRAFLHDDDEVLTSQGTFIGLYVLVK
                     AQGIKLNTVPLKNYHFDLDAIADAVTDKTKIIYLCNPNNPTGTIFTRSEFENFIRKVP
                     DHVLVILDEAYYEFAAHNPEYPDSMRYRLDNVLTLRTFAKAYGMAGVRLGYGLGHEYL
                     IDYVNRIKLPFEPNILAQAAGLAALHDDEYLERTLLNNEEGMAYLTSEFDKMGLQRTP
                     SHANFIMIEMGAQEKVARIHEGLLRHGIAIRPLTAFGLPTCWRVSIGLPEENEMLVKA
                     LKNIL"
     gene            3472..>5062
                     /locus_tag="PKW73_02215"
     CDS             3472..>5062
                     /locus_tag="PKW73_02215"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72124.1"
                     /translation="MAHPKKVFVSALLSIFTSGLVALAPVQAKLTLINTLLSSSQSEY
                     DKGNYLVSLKLSERALEVAQESKAGALAEASALLNVTLNLSALGQYDAAEQDFKKLLA
                     MLDQYKLMRYPVAILAFNNYAAHTRNLGRYTEAEAFLNKAVDLGTRNFGVNSVQNVLS
                     VNNLASLYLSWGKLKEAEALVDRTIELAKAPKAKNTIAGPYSLFNQSKLAELKGDYKE
                     AQVLLQKALEQATTIYGAKHAYVSLVYKGGLAPIAIKNYDFEKAENYARLSLEIDEKT
                     YGKESLGYAGTLVILASIEDKEGKYSQAKEHCQTALATYKKILEGKNTLESINALVVD
                     GSIDGHLGNYEEASGKLAEALNVAKAILPANHIAVANILKESALVELDRGAYKEAERL
                     IEEALSAAKNSVAATNPDLIEIEKSAGHIYRLAGEKKLARAQLEAALAAAEITFSSSS
                     KVSLAVERELAALARELGENEEAATRLKKVISRVEKAEGPESASLIGDLEELAQIQTA
                     LKEGEAARASLARANALMEKLP"
BASE COUNT         1316 a         1456 c         1231 g         1059 t
ORIGIN      
        1 ttgtggaagg gggggcagcc cttttcttga tcattacagg caccatgctg gccatcagct
       61 ttctggtttc ttcaggcatg cttgtctact acaagcaaaa gatgggtttc atatctactc
      121 agatagcgca acaagtaggt atgcatccac aaatggacga catgaccatg accgaaatga
      181 ccagagacat gatggccaag atgagcctgc cgacggcaaa tatgtcggta tcgcgaagca
      241 acaccgatgt atatggacgt cccggtgtgg ccatcacggt gaaaaacggt aagctgcctc
      301 ttttcaccaa cgtagacttc ctgcccatga ccatagacat tcaagacacg gcggtagcct
      361 tcctgtcggc cagcccatcg ggagccgatg cctatctgtg gatgcccaga gggcgcatgt
      421 ccagctatgt cctacccatg gtacgcattc caaacggcgg cacgccgcag ttcagcaccg
      481 taccggtgat ccagtactga agaggccatt gcagttaaga gcgagcacaa gcgggaggca
      541 cggttagagc cactgccttc cctgctgctt aggtctttcc ggctgcttcg gtcttcctgg
      601 ctgcttcggt ctttcctgct gctcaggcct ttccggctga aagcaactgt tcacccacga
      661 tctcacgcca ggtgctgcgg ccgatattgc cgccacaaac cacaacgcct acgcgctcgc
      721 ggggcttgaa ctcaacatat ttggaagaga tggccgcgat tgcgcaggca gcggcacctt
      781 cgatcagcat accttcgtgt tcaaggaaat cgaagacggc ttgcctgatg tcacgctctt
      841 ctactgttac ccactcgtct acgacttcct gcacgagcgg aaaggtaatg gtttcggggt
      901 cgatattacc ggcaatacca tcagcaatgg tctcttgttg caatgtgcgc accacatgcc
      961 ctgactgcac cgctgtgcgc atactgggag aagcggccgg aacgacaccg acgattcgca
     1021 ctttcggatt gataactttg gcgaccagac cgataccgga aataagtccg ccaccgccca
     1081 cagccactac cagggtggag agcttgggaa ccagctcgta catttccagt ccgaccgtac
     1141 cctgaccggc gattacttcg cggttattgt agggtgagat ataaagacct tttttctcaa
     1201 gggcgagacg agcggcatac tgctcggtaa gctccatgtc gtcgccgtgc tgaacgattc
     1261 cgacgctaaa gagttccaac ttggctttct tgacggcggc cacattggta ggcaaacaga
     1321 ccgtagtctc gcgctcggtg gtgcgagccg cttccgccat gcccagggca tgattaccgg
     1381 ccgaggcggt gaatactctg gtgatgccac gctccttggc aaaagtcatg gcattgaggg
     1441 cgcctcgata cttgaagctg cctgtcactt gcaggttctc aagcttcatc cagacttccg
     1501 cctgggaaac atcagacatc cagtgacttt tccgcaaagg cgtattggtg acgaaaccag
     1561 tgatgcgctt acgggccacc agcacatctg agatactgag cggttcttga gccattagtc
     1621 cctttcagtt ccgcctgtac aggttcagcc tgcacaggac gcggcgaatc tctactattt
     1681 aacaataaat ggagcgggta gcgggaatcg aacccgcgtg tgcagcttgg aaggctgccg
     1741 ttctaccatt gaactacacc cgcatatttc aatgatgtct tcgatagcta atttagcaca
     1801 aactttcgcg ttttgcaggg ccggctcaac ttacctgtat atatccgcaa caggtagtca
     1861 cagaaaagtc atgtccccaa atgaatcgcc tgcacatgac aacaactttt tgtcaatgca
     1921 atctatatgt atggccagct taagccaggc tcatcttggc atccatccaa gctgtaggaa
     1981 agtaacggca aatctaaaag ttttctgaac caagctaaaa agctatgtca ttaatttgtc
     2041 gtgaagaact taaagtgcta ctttttatat cgaaccgaat aacaaaaccg gttgtcggat
     2101 gacaagccgg tgttagtttt catcatttgg cgcttgcgga aagctcatca cgaaactatc
     2161 ctggcaccaa agcaaacgag aagctccaac cgagcgggca agaaaggcaa cgaatcgaag
     2221 tttcaaactc attcttgaaa gaacagggaa gccatggcaa cattcattca acaattagca
     2281 tcaatgtccc aacaacttgc cctagtgtcg gatcccaaaa acacaattcg cttcaaccct
     2341 gacatccagc cggaactgcc aaagcgcctg gttccgcctc atgtcgagtc gctcaagcct
     2401 tatcagcccg gtcggccgcc ggagcaattg aagaaagagc tcggcatcga gcgcttcgtc
     2461 aatatggctt ccaatgaaaa tccgctcgga ccgccgcctt ccgctctggc tgccatccag
     2521 gaaaaccttc tcgatatcaa ccgttacccc gacctctgcg gcaccaagct gagagaggcc
     2581 ctggccgaac gctatgccac caaggtagac aacatcgccc tgggcagcgg ttccgagtcc
     2641 accatggcca acatcatccg ggccttcctg cacgacgatg atgaagtgct cacctcacag
     2701 ggaacattta tcggtctcta tgtccttgtc aaagcgcaag gcatcaagct caataccgtt
     2761 cccctcaaaa actatcattt cgacctcgac gccatcgccg atgccgtcac agacaaaacc
     2821 aagattatct acctgtgcaa tcccaacaac cccacaggca caatcttcac acgctccgaa
     2881 ttcgaaaact tcatccgcaa ggtgcccgat cacgtactgg tcatcctcga tgaagcctat
     2941 tacgaattcg ccgcccacaa tcccgagtat cccgattcaa tgcgctatcg ccttgataac
     3001 gtgctcaccc tgcgcacctt cgccaaagcc tacggtatgg ccggcgtcag actcggttat
     3061 ggtctcggac acgaatacct tatagattac gtaaaccgta tcaaactgcc cttcgagccc
     3121 aacatcctgg ctcaagcagc tggtctggct gctttacacg acgacgagta tctggagcgc
     3181 acccttctca acaacgaaga aggcatggct tatctaactt ccgagttcga caaaatgggc
     3241 ttacagcgca ctccctccca tgccaacttc atcatgattg aaatgggcgc ccaggagaaa
     3301 gtagcccgta ttcacgaggg tctcctgcgg cacggcatcg ccattcgccc gcttaccgcc
     3361 ttcggcttgc ccacttgctg gagagtctcc atcggtctgc ctgaagaaaa cgaaatgctc
     3421 gtcaaggctc taaagaatat tctctagaac cgaagcggtt acaatgggac gatggctcat
     3481 ccaaagaaag tatttgtcag cgccctgctc tccatcttca catccggtct ggtagctctt
     3541 gccccggtgc aagctaagct caccttgatt aacactctct tgagttcatc tcagtccgaa
     3601 tatgacaagg gcaactacct ggtctccctc aagttgagcg agcgggccct ggaagtagcc
     3661 caggaaagca aggccggcgc cctggctgag gccagtgcct tgttaaacgt aacccttaac
     3721 ctgagcgctc tcgggcaata tgatgcggcc gagcaggact tcaagaagct tctggccatg
     3781 ctcgatcagt acaaactgat gcgctatccc gtggccattc tggctttcaa taactatgcc
     3841 gcccatacga gaaacttggg gcgctacacc gaagccgaag cctttttgaa caaggctgtt
     3901 gatcttggca cccgcaactt cggagtcaat tcagtacaga atgtcctcag cgtcaacaac
     3961 ctggccagtc tttatttaag ctggggcaaa ctgaaggaag ccgaggcgct ggtagaccgc
     4021 accatcgaac tggcaaaagc gcccaaggcc aagaacacca tcgccggccc ttattcgctt
     4081 ttcaaccagt ccaaactggc ggaattgaaa ggcgactaca aggaagccca ggtgctcctg
     4141 cagaaagcgc tcgagcaagc caccactata tacggtgcca aacacgccta tgtctccctg
     4201 gtctacaaag gcggtctggc tcctatagca atcaaaaact acgacttcga aaaagccgaa
     4261 aattacgccc gcctctccct tgaaatagac gaaaaaacct atggcaaaga gagcctcggc
     4321 tatgccggca ccctggtaat tctggcatcc atcgaagaca aagaaggaaa gtatagccag
     4381 gccaaagagc actgccagac agcacttgct acttacaaga aaattctgga aggcaaaaac
     4441 accctcgaat caatcaatgc cctcgtggta gacggcagta tcgatggaca tctgggcaac
     4501 tacgaggaag cctccggcaa actggcggaa gcactcaacg tagcaaaagc aatcctcccg
     4561 gccaaccata tcgctgtagc caatattctc aaagagtcgg cactggtaga gttggatcgc
     4621 ggcgcctaca aggaagctga gcgacttatt gaagaagccc tcagtgcggc caaaaacagc
     4681 gtggcagcaa ccaatcccga cctgattgaa attgaaaaga gtgccggaca catttaccgg
     4741 ctggccggcg agaagaaact ggcccgagcg caactggaag cagccctggc agcggccgaa
     4801 atcaccttct caagcagctc aaaagtcagt ctggccgttg aaagagaact ggccgcgctg
     4861 gccagagaac tgggtgaaaa cgaagaagcg gcgacccggc tgaaaaaagt catcagtcgg
     4921 gtggaaaaag cggaaggtcc ggaaagcgcc tcactgatag gcgacctgga agaactggcg
     4981 caaattcaaa ctgccttaaa agaaggcgag gccgcccggg ccagtctggc aagggccaac
     5041 gccctcatgg aaaaactgcc cg
//
LOCUS       DAOQNZ010000078         5049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6868_length_5049_cov_3.462956, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000078 DAOQNZ010000000
VERSION     DAOQNZ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6868_length_5049_cov_3.462956"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..387)
                     /locus_tag="PKW73_02220"
     CDS             complement(28..387)
                     /locus_tag="PKW73_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72125.1"
                     /translation="MRMAQFYGLSPRGKKLVLKKVMVREVGTMFLPDGRSVKFDRRRP
                     VPAARKQVIGDIKTLNPQLPLAQLHRYTMPDGKVYEEYIQEIIHSGGPCYFLALRTAD
                     GKPVRHTLWTDEEVSQY"
     gene            1335..2336
                     /locus_tag="PKW73_02225"
     CDS             1335..2336
                     /locus_tag="PKW73_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNA72126.1"
                     /translation="MTDVLCLGEILVDWVCTTVGAELDRAQHFTKAAGGAPANTAVGL
                     ARQGVATAFIGRTSDDAFGRWLRSILEEEGINVEAAILDPTAQTRMAYVVTTSSGDRK
                     LAEFSRIACADTKLQPNDLKQHLFAAASVLHFGSISLIESPAQESTKKAVEMARANKL
                     LVSYDPNVRISLWPSRETCRKTILNTLSWADVVKINEDELEFLTGSRDTQAADRLREE
                     HNVPLLIITLDSRGAYFTTAGHGGKHVPGFQVELVEATGAGDGFNSGVIAGLLPFIKG
                     AADRRQALLSMEQNQLFEIIQAANAIGAITCTKPGAIPALPKRDEIDSFLAKMAVKS"
     gene            2472..3467
                     /locus_tag="PKW73_02230"
     CDS             2472..3467
                     /locus_tag="PKW73_02230"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNA72127.1"
                     /translation="MNKHAGVDGVRSVAAQNLGETGEKGKVVSLKKDGSRKLTLDPDK
                     SYKTVMKRTADTPIEAVKAAPGLSFNGDVDLDLASKIAALEAIEQKTKLICPACGAVS
                     TELRLSCQNCGNYFDGHSGAADVRLAALSSTGGLSREEQEKLAQKSYLTRRFLAKVID
                     SVILLSLVACQYLIFFAVARSLTVIPSMAFLALNFFFWGMPCLTLMTLLGYHALFEAS
                     QVQASPGKLLLGLFVEDLNSQIVRSEAILFKSLVAYSPLVVALGVYAYFYHVRLQYGL
                     SLDAPTSTVLALSALTCMVTYGLMHLIVGKEEKRRTVLDVMAGCRIKERSKQLPR"
     gene            3833..4255
                     /locus_tag="PKW73_02235"
     CDS             3833..4255
                     /locus_tag="PKW73_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72128.1"
                     /translation="MLQASIGPGLVINAIMAADFFNVQDDFDLARFTRLDSDGYINYV
                     TVTTELVQNAPPPSALPDSKEAGLACMEHLPPVLAALAQSLLQLKCRVQFHQPGQRSD
                     QCLATLERTDYIQSMNRLVVHRVKIHFHRRDGRYPVSA"
     gene            4325..4720
                     /locus_tag="PKW73_02240"
     CDS             4325..4720
                     /locus_tag="PKW73_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72129.1"
                     /translation="MSGANSGIGKGFAILLAVVTGVIAVVALFSLFLGSFFDSAVVKI
                     ACSALLPVVAAYLFYQPRLVCFFTGASGVAAYYAAAYAAAAVGTGLGALVLGGVAGII
                     TFVVAFLVGLLLSGKLSPGADSWRKSKAK"
BASE COUNT         1039 a         1368 c         1366 g         1276 t
ORIGIN      
        1 ctcagcgccg gattgctcta gcgctgatta gtactgagaa acctcttcgt cggtccagag
       61 cgtgtgccgc accggtttgc catccgccgt gcgaagggcg aggaagtagc agggaccacc
      121 gctgtggatg atctcctgga tgtactcctc gtagaccttg ccgtccggca tggtgtagcg
      181 atgcagttga gccaggggca gttgcggatt gagggttttg atgtccccaa tcacctgctt
      241 cctggctgcc ggcaccgggc gacgacggtc gaacttaacc gaccggccat ccggcagaaa
      301 catggttccg acttcgcgga ccatgacctt cttgaggacc aacttcttcc cacgagggga
      361 aagaccgtaa aactgtgcca tgcgcatgat gagactcctt agctatcgtt ttggggcgtg
      421 tgtggtctcc gtctcgttct cccgagcccc attggtcgga gccgaaatcc cacggaatgc
      481 gggtaaatcg gtgagtcgga gttgaaagct gtaaagccac ggaccgaacc ggtcgggagg
      541 cgacccaaaa gggcgcttcc caccggtggg tgacgtgtgc tgcttgccgg ttaatcgaga
      601 ggggcttgtc gttgacctga cgacggagca actcttgatg cgggcatttc cttcagtgct
      661 gcttgttagc aggaccacta gctagcgata gtcggctcgt aagagcagac gccaagtgag
      721 cgcgatcaac agatcggacg gacatcaatt tgcagacagg cagaatcggc agttttctca
      781 ggctctacac acacacctgg gaattccctg gtgctctcca acttcaggct ggattttggt
      841 tattaatggt tcttgagatg aaggtccttg gttggaagag gagagtgtgt atattgacaa
      901 cctagtgaaa gcaccgatgc cctggcaatt gatctgtgat taaaggcttg tcatattgct
      961 ccccgatggc ccaagctgta agcagtttga ccggtatgaa attagcgcag atagcgaggc
     1021 ttttccttac cgtgacgaaa ctgttatcgc actttccggt cccgtcattg ccgcatggtt
     1081 ctcaagcgag acaaatgcac tggctgcgct agcacttggc attggtgaaa ggcagctttt
     1141 aggctaaata attggcttgt tacatccttt aacgtcagtc gggaagggca aattctcagg
     1201 cctacttaga ctttataatc ctctggttgt tcgcggatac tcgaacagat atctaactgg
     1261 tgcgtccgga gaaccggtga tgagatctgt tgatattcgc gtatgtcctt atcttccgcc
     1321 aggagaatcc ttcgatgacc gacgtactgt gccttggtga aattcttgtg gattgggtct
     1381 gtaccacagt aggcgccgag cttgatcggg ctcagcactt taccaaggcc gccggagggg
     1441 ctcccgctaa cacggctgtc ggcctggctc gccagggtgt cgctacggcc ttcatcggtc
     1501 gcacttccga cgacgctttc ggcagatggc tgcgctccat tctggaagag gaaggcatca
     1561 atgtagaggc cgccatcctc gaccccaccg ctcagactcg catggcttat gtggtcacaa
     1621 catcctccgg tgatcgcaaa ctggccgagt tttcccgcat cgcctgcgcc gacaccaaat
     1681 tgcagcccaa tgacctcaag cagcatctct ttgccgccgc ttccgtactg catttcggct
     1741 ccatttcatt gatcgagagc ccggctcaag aatccaccaa gaaggccgtg gaaatggccc
     1801 gggccaacaa gcttttggtt tcttacgatc ccaacgtgcg tatcagtctc tggccttccc
     1861 gggaaacatg tcgcaaaacc attttgaaca ccctttcctg ggccgatgta gtgaaaataa
     1921 atgaggacga gctggagttt ctcaccggct cccgtgacac ccaggctgcc gatcgcttgc
     1981 gggaagagca caatgtgccg ctcttgatta tcactcttga tagtcgcggt gcctatttca
     2041 ccaccgccgg ccatggcggc aagcatgtac ccggcttcca ggtagagctg gtggaagcca
     2101 caggcgccgg tgacggtttc aattccggcg ttattgccgg tttgcttccc ttcatcaaag
     2161 gcgcggccga tcgcaggcag gctctcttga gtatggaaca gaaccaattg tttgagatta
     2221 tccaggctgc caacgccatc ggcgccatca cctgcaccaa acccggtgca attccggccc
     2281 tgcccaagcg tgatgaaatt gacagcttcc tggcaaaaat ggctgtgaag agctgataaa
     2341 cagttcgcaa aaatgaattg acctgttcct taaaggatgt taaatttctg ccactggttt
     2401 atttcctggc gttaatatcc tgactaccag aaagtaagtt tctttctgac taaatggttg
     2461 aaacagtaaa tatgaacaag cacgctggcg ttgatggcgt tcgttccgtt gcggctcaaa
     2521 atttgggaga aacaggcgag aagggtaaag ttgtctctct caagaaagac ggctcaagga
     2581 agctcactct cgaccccgac aagtcataca aaaccgtcat gaaacgcacg gccgatactc
     2641 cgattgaggc agtgaaagct gctccaggct tgagtttcaa cggtgacgtc gatctcgatc
     2701 tggccagtaa gatcgctgct ctggaagcga tagagcaaaa gacaaagctg atttgccccg
     2761 catgtggagc ggtttctacg gagctgagac tctcctgtca gaattgcggt aactattttg
     2821 acggccattc cggtgcggcc gatgtgcgcc tggctgctct gagcagcacc ggcggcttga
     2881 gccgtgaaga acaggaaaaa ctggcccaga aaagttattt gactcgtcgt tttctggcca
     2941 aggttatcga cagcgtgatt ttgctctctc tggtggcctg ccagtacctg attttctttg
     3001 cggtagctcg ctctctcact gtgattccga gcatggcctt tctggctctc aatttcttct
     3061 tctggggtat gccctgtctc acccttatga ctttactggg ctaccatgcg ctctttgaag
     3121 catcacaggt gcaggccagc cccggtaaac tgttgctcgg gctctttgtg gaagatttga
     3181 acagccagat cgtgcgttcg gaagcgattc tcttcaagag ccttgtagcc tattcgccac
     3241 tggttgtagc ccttggtgtt tatgcctact tctatcatgt acgtctgcaa tatgggctta
     3301 gcctggatgc gcccacttct accgtgcttg ccctcagtgc actcacctgt atggttactt
     3361 acggactcat gcacttgatt gtgggcaaag aagagaagcg ccgcaccgtt cttgatgtca
     3421 tggccggctg ccgcatcaaa gaaagaagca agcaactgcc tcgctagtgg ccattctctg
     3481 aaaatcctgc aatgatctgc tagctgcgcg aactcaagaa ggtctaatca gcctctccat
     3541 tccttcggag aggctgattt tggcagggag ttaatcctgg caagtcatag ttaaacagtt
     3601 ctcctggagt tgcctggggg cttttgttag gttgatctgg ctttcctaat tcctaagttc
     3661 acaggttcct ctcccccata gtcgacaaat tcgccttcct ttcaccaagt ccggttcgga
     3721 tcagctccct gatggtctgt gtcttgtgcc ggtttattgg ttcgtcgcat gtggtgatga
     3781 ggtttttcaa tcagtccttt caagtaaagg acagtcaagt aaaaggacag ctatgctgca
     3841 agcttccatc ggacccggct tggtcataaa cgccatcatg gcggccgact tcttcaatgt
     3901 gcaggacgat ttcgacctgg cacgtttcac caggctggac agcgacggtt acatcaacta
     3961 tgtcaccgtc accacggaac tggtacagaa cgcccctccg ccttctgccc tgccggacag
     4021 caaggaggct ggtctggcct gcatggagca cctgccgccg gtactggccg cgctggcgca
     4081 gtctctcttg caactgaagt gccgggtgca gttccatcaa ccggggcagc gttcggatca
     4141 gtgcctggcc accctggagc gtaccgatta catccagtcg atgaatcgcc tggtggtgca
     4201 tcgggtcaag attcactttc atcgccgcga cggtcgctac ccagtgagcg cctgaagcgg
     4261 ttccgcctgg tgccctcaga cggggcgcct ggtcctcttc atcaatccaa ttggagcaca
     4321 caatatgagt ggtgcaaatt ccggcatcgg caaaggcttt gccatccttc tggcggttgt
     4381 caccggcgtc atcgccgtgg tggcgctgtt ttccctcttc cttggttcct tcttcgacag
     4441 cgctgtcgtc aagattgcct gctcggcgct tctgcccgtg gttgccgcct atctcttcta
     4501 ccagccgcgc ctggtctgct tcttcaccgg tgcgtccggc gtcgccgctt actatgccgc
     4561 cgcctatgct gctgccgctg tcggcaccgg actgggcgct ctggtccttg gtggcgtggc
     4621 aggcatcatc accttcgtgg tggctttctt ggtcggtctt ctcctgtccg gcaagctgag
     4681 ccccggggct gattcctgga ggaagtccaa ggcgaagtaa gaagtagtcg ggctcgcgct
     4741 tttgctattt ccccgaagga acaggcccgt acggctgttc cttctcttga ggggctttgt
     4801 aatcgggatg cagcctcgtt ttctttatct ctaagcttga tctactcatt aatacagtat
     4861 ggctggtttg cgtctcaaaa atgagggatg caaccccgct gttatttttt gtggagcgtg
     4921 ttggcaaggg tagttactgg gccgatttac tttggggggg gctgagtcag cgccagcttt
     4981 cacctgaata gttgtaccgt tcagccaatc gacaaacaaa aggaattgtt gatttggcgc
     5041 aagcaaacg
//
LOCUS       DAOQNZ010000079         5016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6947_length_5016_cov_3.445676, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000079 DAOQNZ010000000
VERSION     DAOQNZ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6947_length_5016_cov_3.445676"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKW73_02245"
     CDS             <1..437
                     /locus_tag="PKW73_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA72130.1"
                     /translation="CALDVSKTLLAACALGLCQEGLSLAAQRANTREQFGAPIAKNQG
                     VQWKLADFSTDAAAARLLTYRAAWSRDGAPAEFRKFAAMAKSYAARTARLHSGEAVQI
                     FGMLGASCDEPLERIYRDAKLTEIFEGTSELQKVILKEELGV"
     gene            506..1735
                     /locus_tag="PKW73_02250"
     CDS             506..1735
                     /locus_tag="PKW73_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HNA72131.1"
                     /translation="MMLKTGGFRRTILTILPGLVLSLTAGAWAWAAGADAYSTGVQLL
                     TARKFPQAAQYFSQAVKVSPRQGMALYYLGLSKHYQGDRQGAMESYSRVLSEYPGTEA
                     CNLAVRGMSSIDPSILQQLGLAGGGQAAGRRQQRQEASQGTDDLSMPPECRVNFTKES
                     SCMIVDGRVNGRSTRMLFDTGAENVVFGMNHIQEMGINLGKSEQSVNVTGSGSRGSQK
                     AGVYLVELSLGNIVRKNFPILVMEHADNYPIIGQRFFDELKYTVDNNSNCITFQRRTG
                     QVASNAPLTAYDVPFERDGKEIRVMTEIEGKKVPMWLDTGASWVSMSASQAQACGIAV
                     PDDARVVQTRGVNGLSRTRFVRVRSMRLGPVDKRDYEIGVADSLPSPYPLLGGDFLQN
                     QQFTVDYDRGVIHFGRH"
     gene            1752..2534
                     /locus_tag="PKW73_02255"
     CDS             1752..2534
                     /locus_tag="PKW73_02255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA72132.1"
                     /translation="MPTISLVIVAQNEERTLGAVIDAAKELVCEIIVVDSGSTDSTIA
                     IATDRGAKVIHQDWLGYARQKNFAIDLAAGEWILSLDADEILTPPLVQELKALISSPD
                     FARYDGYKIPRVLYIGETPIFHGGFYPDAQLRLFKKGSGRWGERLVHEAIKMDGPKAV
                     LKNHMDHYSYKTVDDFAAAMDKYARLSAREFYQRGKYQWRAHPINLVLHPLWTFLQRY
                     FLRGGILDGRLGLRLAVIYSDYVRKKIVYLRELARDKQADCS"
     gene            2709..3962
                     /locus_tag="PKW73_02260"
     CDS             2709..3962
                     /locus_tag="PKW73_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNA72133.1"
                     /translation="MAGKNRAPALIAALALVFLPVGTRAAGVDPYEAGIKQLSAKQFA
                     QAGLSFQQAVKANPKNAMAFYYLALARHYQGDRSGAMNAYSRVISTFPGSQASSLALR
                     GMASIDPSIMQKLGLASAGGNSGAGAAPSGGGRNYGSNYTASSSSSSNDTVPDECRVY
                     FTKDANCFVMDVRLGNRPLKMMFDTGAESTVVGKNHLQELGLPLPTGPSVGTAHGVGE
                     GGGQRFWVQKMDVTLGGITRRNFEVCVQEHMPGYPLLGQTFFHDMRYTVDNGANSIHF
                     MKKKSDRGSVYSSLSRDPNVVPFTREGNEIVVVTEVNGRKLPMYFDTGASGVAFSPEH
                     LSQVGLTIPDDARTSMSQGIAGNSLVRNFPVGSLKLGPIEKRDFEIGVTMSSHMPHPL
                     LGQTFYNDYQYTIDYDKSVIHFMRR"
     gene            complement(3973..4380)
                     /locus_tag="PKW73_02265"
     CDS             complement(3973..4380)
                     /locus_tag="PKW73_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72134.1"
                     /translation="MAPTRIQLALAAAAVILCTPQAVMAQEKKETTGEQTEWMKKAFA
                     LHVKSLSFQKGSAHFVTDGFFQKAGKSDFLLSRGDAFEGQSDHHASSTFTCTGVNEKE
                     GVSIQYQSHFDHRSFGKNLISTDKGNLILPWKK"
     gene            4587..4841
                     /locus_tag="PKW73_02270"
     CDS             4587..4841
                     /locus_tag="PKW73_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNA72135.1"
                     /translation="MKKLFVGNLSFNLTESDLEALFAPSGQVTSVSIPTDRESGRKRG
                     FAFVEMNTEAEGEAAIRNLDGKEVDGRTIAVNVSKPKPRY"
BASE COUNT         1059 a         1298 c         1418 g         1241 t
ORIGIN      
        1 cttgtgccct tgatgtctcc aagaccttgc tggctgcctg cgccctcggt ctctgccagg
       61 aggggctcag tctggccgct cagcgcgcca atacccggga acagttcggc gctcccattg
      121 ccaaaaatca gggtgtgcag tggaaactgg ccgatttctc tacggatgcc gccgccgccc
      181 gcttgctcac ttaccgggct gcctggtcca gagacggtgc ccccgccgag tttcgcaagt
      241 tcgctgccat ggctaaaagt tatgcagccc gcaccgcccg cttgcacagt ggcgaagccg
      301 tgcagatctt cggtatgctg ggagcatcct gtgatgagcc cctggagaga atttaccggg
      361 acgccaagct gacggaaatt ttcgaaggta cttcggaatt gcagaaggta attctcaagg
      421 aagagcttgg agtctagttc gactccactg tactgccgct tcttcagggt acatactgtc
      481 tggttggttg gctgttaagg agctgatgat gctgaaaaca ggcgggttta ggcgtacaat
      541 cctgaccatt ttgcctggtc tggtgttgtc tctgaccgca ggcgcatggg cctgggctgc
      601 cggtgccgac gcctacagca ccggcgtgca actgctcact gccaggaagt ttccgcaggc
      661 tgctcagtat ttcagccagg cggtgaaggt cagtccccgc cagggaatgg ctctttatta
      721 cctggggcta tccaagcatt atcaggggga caggcagggt gccatggagt cttactcccg
      781 ggtcctctcc gaatatcccg gcacggaagc ctgcaacctt gccgtgcgcg gcatgtccag
      841 tattgatcct tccattttgc aacagcttgg tctggccggc ggcggccagg ctgccggtcg
      901 gcgccagcag cggcaggaag catctcaggg tactgatgat ctttccatgc cgccggaatg
      961 tcgggtcaat ttcacgaaag aaagcagttg catgatcgtg gatggccggg taaacgggcg
     1021 ctccacccgc atgttgttcg acaccggcgc tgaaaatgta gttttcggta tgaatcacat
     1081 ccaggagatg ggcatcaatc tgggcaagag cgagcagtcc gtcaatgtca ccggctccgg
     1141 cagccggggc agtcagaaag cgggtgtcta tttggttgaa ttgtctctgg gcaatattgt
     1201 gcgcaagaac ttcccgattc tggttatgga gcatgccgac aactatccga tcatcgggca
     1261 gcgcttcttc gacgagttga agtacaccgt cgacaataat tccaactgca ttacctttca
     1321 gcgccgtacc gggcaggtcg ccagtaatgc gcccctcacc gcttacgatg taccttttga
     1381 aagagacggc aaagagattc gcgtcatgac cgagatagag ggaaagaaag tgcccatgtg
     1441 gcttgacacc ggtgcttctt gggtctcaat gagcgcctcc caggcccagg cttgcggtat
     1501 tgctgtgccc gatgatgctc gagtcgttca aaccaggggc gtcaatggct tgagtcgcac
     1561 tcgctttgtg cgggtgcgct ccatgcgcct tggtcctgtg gataagcgcg attatgaaat
     1621 cggtgtggcc gatagtttgc cgtcgcccta tcccctttta ggcggagatt tcctgcagaa
     1681 ccaacagttc accgtggatt acgacagagg tgttattcac ttcgggcggc attgagccca
     1741 ggaagtaagt gttgccaacc atctcgctag tcatagttgc ccagaacgag gagcgcacac
     1801 taggtgccgt aatcgacgct gccaaggaac tggtctgcga gattattgtg gtagactccg
     1861 gctccaccga tagtaccatt gctattgcca ccgaccgtgg tgctaaggtc attcatcaag
     1921 attggcttgg ctatgccagg caaaagaatt ttgccataga tctggcagcc ggtgagtgga
     1981 tcctcagttt ggacgcggat gaaatattga caccgcctct cgtgcaagag ttgaaagctc
     2041 tcataagttc acctgatttt gccaggtatg acggttataa aatcccaaga gtgttataca
     2101 taggcgaaac tcccattttc cacggcggct tttatcccga tgcccagttg cgtttattta
     2161 aaaagggcag tgggcgctgg ggcgagcgcc ttgtgcatga agcgattaag atggacgggc
     2221 ccaaagccgt cttgaaaaac cacatggatc attattccta taagaccgtg gatgacttcg
     2281 ccgccgccat ggataagtat gcccggctct cggcccggga attctatcag cgtggcaagt
     2341 accagtggcg agcccaccct attaatctgg tgctgcatcc gctctggacc ttcctgcaaa
     2401 ggtacttctt gcgcggcggc attctggacg gcagactggg tttaaggctg gcagtcatat
     2461 acagcgacta tgtgcgcaag aaaatagtct atttgcgtga actggccagg gataagcagg
     2521 cggattgttc gtaaattaat tggggcggag ccggcattag aacagcgggg atcggcaggt
     2581 atttagctcg gggctgggta aatatttaga gaccgatgct ttgataaatt ctgctgggta
     2641 gagtgcatag gtccgtattc aggcagtgtg tttgaatagt gagaaaggaa agccttaagg
     2701 aggataaaat ggctggaaag aatcgggctc cggctctcat agctgcactg gccctcgttt
     2761 tcttgccagt gggtacccgg gctgccggcg tagatcccta tgaagcgggc attaagcagc
     2821 tttccgccaa acagtttgcc caggccggtc tgtcttttca gcaagccgtc aaggctaacc
     2881 ccaagaatgc catggccttt tattatctgg ccctggccag gcactatcag ggcgaccgtt
     2941 cgggagccat gaatgcctat tccagggtca tttctacttt tcccggttcc caggccagca
     3001 gcctggccct gcgcggcatg gcttctattg atccatccat tatgcagaaa ttgggtctgg
     3061 cctcagcagg cggcaactcc ggggccggtg ccgcgccttc cgggggcggg cgtaactacg
     3121 gcagcaatta cacggccagt tccagtagtt cttccaacga tacagttccg gatgagtgcc
     3181 gggtttactt cacgaaggac gcaaattgct tcgtaatgga tgtgcgcctc ggcaatcggc
     3241 cattgaaaat gatgtttgac accggcgccg agtctaccgt ggtcgggaag aatcatttgc
     3301 aagaattggg tctacctttg cccacaggcc cttccgtcgg tactgcccat ggtgtaggcg
     3361 agggaggcgg tcagcgtttc tgggtgcaga agatggatgt tactttgggc ggtattacgc
     3421 ggcgaaattt tgaagtatgt gtgcaggagc atatgccggg ctatccgctc ttgggtcaga
     3481 ctttcttcca tgacatgcgt tacaccgttg ataatggtgc caacagcatt cattttatga
     3541 aaaagaaatc cgacagaggc tcggtctatt cttctctcag tcgggatccc aacgttgttc
     3601 ctttcacccg ggagggcaat gaaatcgttg tggtaactga ggtgaacggc cggaagctgc
     3661 ccatgtattt tgatacaggg gcgtccggtg tggccttttc ccctgaacat ctaagccagg
     3721 tggggcttac tattccggac gatgcccgca cctccatgag tcagggaata gcaggcaact
     3781 ctcttgtccg caactttccc gtgggttcat tgaagttggg gcccattgag aagcgtgact
     3841 ttgaaattgg tgtcacgatg tcctctcaca tgccgcatcc gcttctgggt cagacctttt
     3901 acaacgatta tcaatacacc atcgactacg acaagagcgt cattcacttt atgcgccgtt
     3961 agggtcgcgg tctcatttct tccagggtag tatcagattg cctttatccg tgctgatcag
     4021 attcttgccg aaggacctgt ggtcgaaatg cgactggtac tgaattgaga caccctcctt
     4081 ttcattcacc ccggtgcagg tgaaggtgga agaggcgtgg tgatcacttt gcccctcgaa
     4141 ggcatcgccc ctggatagga ggaagtcgct cttgccggct ttctgaaaga agccgtctgt
     4201 gacgaaatgg gcactgccct tctggaatga gaggctcttc acgtgaaggg cgaaggcttt
     4261 cttcatccat tccgtttgct cgcctgtggt ttcctttttc tcttgtgcca tgactgcctg
     4321 gggggtgcaa aggatgacgg cagccgctgc cagagcaagc tgtattcttg tcggtgccat
     4381 tttcatggtc ctcctcatga gggctgtctc gggtgattgt atttctcttt ttcccttctt
     4441 gccagaaacg ccgctctttg ttctctatat tgacaatcgc ttgtaagtag gagtaatacc
     4501 tgccatacta cctccggggc tacaaagcta gagagcctcg tgcgcagaaa gagttttatt
     4561 tgcgcataca cgctatacga gattcaatga agaagttatt tgtcggcaac ctgtctttca
     4621 acttgacgga gtccgatctg gaagccctct ttgccccttc cgggcaggtt acctcggttt
     4681 ccatccccac cgaccgtgag agcggtcgca agcgcggttt cgcttttgtg gaaatgaaca
     4741 cagaagccga aggcgaagct gctatccgca accttgatgg taaagaagtt gacgggcgca
     4801 ctattgcagt caacgtctcc aagcccaagc cgagatacta agccgcagct tccttaatca
     4861 aaggaagagg tgattagagt gaagctgtcg agttagatgc tcgctggttt tgtcgcgggc
     4921 ccatccggta agctgcaggc ttccgggtgg gcttccggat tggcctaagg gttggtttaa
     4981 agatcagtac cgccccggtg ccgaactacc tgtttt
//
LOCUS       DAOQNZ010000080         5010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_6962_length_5010_cov_4.084965, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000080 DAOQNZ010000000
VERSION     DAOQNZ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6962_length_5010_cov_4.084965"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..4247)
                     /locus_tag="PKW73_02275"
     CDS             complement(27..4247)
                     /locus_tag="PKW73_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72136.1"
                     /translation="MAEKPEANSQNEADTQPAQSRQALSLEATQPAVKPSDNLETVKP
                     ESDKSQGNSRPLTDATQTLPASTEKYLPELQITSDDTVRGSVRELDRALSKTTLGIAN
                     PDGKSVLTSLASLSDGERAKLEALYKSELGPERDLRRDLQKLSGGDQHRALALLDKKD
                     GKTDDAAHLQAALSERDPQKRDGQIRLLYRTLNPEQIQTMESRFSEKGGSVEQALRQA
                     GVPESTISAIETYKKAARENRPVSGEELSQLARLGARTGNRDLIIEAVAGTTPEAKQA
                     REMLKDDRAFNAGILRNFKEKAPATNLTEAELKEPSNRVDPVLRDYLQEGRVRLSTVA
                     EANSGYFFGNKANTDLAVKHASADERQEFKRGYDLDRRLKDNGEDGSKLSPDEQKSLG
                     FYRNLQASLEKGGNRGDAIRWQDTLLNGRETIVSELGKTEKDGLLWGSSHSSADALTA
                     AGKLNEKDWRTLRNAEHGQTPAEKAEGAAFKRQVEESVGRFAGNPEESARIMRMLSEK
                     ANKETFADAQKVGRSLDETLSDARGGNHKQQLEDSLKAITTMTPEQARQYKEDAQVRK
                     QVDDELAKGPAEVRPLSQRLLKQVAESGEPPVLKGFDKIENSALSGKPLTIKETQELL
                     REPEVAKRLGKSDVDLTPAEKRASLTLDSAIYNGLAARRLNERPQLLGEAQPGDSRSL
                     LLQAGAIRRELAQNGGQLSLEQQYKLNLPMQDVLGNLASAPKAEQERFLKEVSTNPQF
                     QDLAANVAKQGEVRLEDRAKLYSLDGKDSKELAKTLGQASPQELADARAGWAWKYQGD
                     LATTVLNRAGDGEKPALKAALTPADDGRAGLYENYDKYLSNRGGFTLDGTALTLERAG
                     QLHQEAFKAYDQARQGLPPAKQKELDEFFGKSLQDHKDSKEKQTQILLDAAITAATLG
                     SGAPLSVAKVAARIAAGGLTESAGLALTRGEFKAENFAQDFLQGGLAAYAGKTARVAG
                     DKVASSLERPKLTGAAPTKVADEAAEIGAPKQARLETPAHTTRIEKESAELGLTRPLE
                     AKALQSGLPVKIEGASKDFAEKVGRSVESLPPEIAEKLKKEGISIEVVGNIHNYTTDQ
                     AYLSTPVRGQPGKTQADSVGFFDRANKKIILVENSLNGQAYWPGDTVQRAVSHELGHA
                     VDSYTVGDALKSQAFERSAQPGRPLVGRQISDLPEFQKAVYADLQELSKTMAKATPEE
                     RQAIGKIVEYNLANNGHELKKLGLDGIGTHHDTPRLHDQNGIRHFGGRHSDARSEAFA
                     DVHSDLLYGDDRFTKYFPNVTRLIRDDVLKLPGTSFDQVTGESLQKLGLKPIAQHGER
                     LWLESADKKSFAVINEGKLVGMKVGGTDYELAYNSQGQLARIETSSRRYEKIDGSWHK
                     TDASGSEKLTQAPVLTRLGNGRLQFFER"
     gene            4437..>5010
                     /locus_tag="PKW73_02280"
     CDS             4437..>5010
                     /locus_tag="PKW73_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72137.1"
                     /translation="MNAARLLRELGASFWDDLTGAFSLRNWQDCLAKLSITSRKQLIE
                     NLLGIIPQVAIFYLVLTPVVAMPLYNKMLFFPLKQHAIDFAELAGVAKVNVQIVGENS
                     RKLSAWYFPVDGARGAVIISHGNGGNISHRGPLIELLLRQKMSVLAYDYQGYGESEGE
                     PSIDNICRDGLAAYDYLVTQKGVKPEKIVLY"
BASE COUNT          857 a         1378 c         1427 g         1348 t
ORIGIN      
        1 ttgaagccag gtatctggac gcctccttaa cgctcaaaga attgcaagcg gccgttaccg
       61 aggcgggtta gaaccggtgc ttgggtgagt ttttcgctgc cggaggcgtc agttttgtgc
      121 cagctgccgt caatcttttc atagcgccgg ctgctggtct cgatgcgggc tagttgaccc
      181 tgactgttgt aagccagttc atagtcggtg cctcctactt tcatgcccac cagttttcct
      241 tcgttgatga cggcaaaaga cttcttatcg gccgactcca gccagagccg ctctccgtgc
      301 tgggcaatgg gcttcaatcc cagcttttgc aggctctctc ctgttacctg atcgaaggaa
      361 gtgccgggca acttcagtac gtcgtcacga atcaatctgg tgacattggg gaaatatttt
      421 gtgaagcgat catcgccgta gagcaggtcg ctgtgcacat cggcaaaagc ctcactgcgt
      481 gcgtcggagt ggcgtccgcc gaaatgtctg atgccgtttt gatcatgcag gcgtggtgtg
      541 tcgtggtgag tgccgatgcc gtccagaccc agttttttca gctcatgacc attgtttgcc
      601 aggttgtact ccacgatttt tccaatggcc tgccgctctt ccggtgtggc tttagccatg
      661 gtcttagaaa gctcttgcag atcggcgtag accgcttttt gaaattcagg caagtcggaa
      721 atttgtcgcc caacgagggg acgaccgggt tgggccgatc tttcaaacgc ctgcgatttc
      781 aaagcatcgc ccacggtgta gctgtctacc gcgtgtccga gctcatggct gacggctctt
      841 tgcacagtgt ctccgggcca gtaggcttgt ccgttcaggg aattttccac caggatgatt
      901 ttcttgtttg ctctgtcgaa gaatccgacg gagtcagcct gggtcttgcc cggttggcct
      961 ctcaccggcg tacttaaata agcctggtcg gtggtgtagt tgtggatgtt gcctactact
     1021 tcgatggaga ttccttcctt cttcagcttt tctgctattt ccggcggcag ggattcaaca
     1081 cttctgccga ctttctctgc gaaatccttc gaggcaccct ctattttgac cggcagcccg
     1141 ctctggagcg cctttgcttc caggggacga gtaagaccaa gttcggcgct ttctttctca
     1201 atgcgagtgg tgtgggctgg cgtttctagc cgagcctgct tgggcgcgcc tatttcagcc
     1261 gcttcatcag ccactttagt cggtgccgct ccggtaagct ttggcctctc cagagaactt
     1321 gccaccttgt cgccggctac tctggcggtc ttaccggcat aggcggccag tcctccctgc
     1381 aggaagtcct gggcgaagtt ttctgctttg aactcaccgc gagtaagagc cagaccggcc
     1441 gactcggtca atcccccggc agcgatgcgg gccgctactt tggcgacgct gagcggcgct
     1501 cccgagccta aagtggcggc agtgatggcg gcatcgagaa gaatctgggt ttgcttctct
     1561 ttcgagtctt tgtgatcttg cagcgactta ccgaagaact catccagctc cttttgcttt
     1621 gcggggggaa gcccttgccg cgcctgatca taggctttga aagcttcctg atgcagctgt
     1681 ccggcccgct ccagggtgag cgccgtgcca tctaaggtaa aaccgccacg gttgctcaga
     1741 tatttatcgt agttttcata gagcccggct ctgccgtcgt cagcaggtgt cagtgccgct
     1801 ttcagggcgg gcttttcgcc gtcgccggcc cggttcaaga ccgtggtggc aagatctccc
     1861 tgatacttcc aggcccagcc tgctctggcg tcggcaagtt cctggggcga ggcctgaccg
     1921 agggttttgg ccagttcttt tgaatctttg ccgtccaggc tatagagctt cgctctgtct
     1981 tccaggcgca cctcaccttg tttggctaca ttggcggcca ggtcctgaaa ctgagggttg
     2041 gtgctcactt ccttcaggaa gcgctcctgt tcagctttag gggccgaggc cagattgccc
     2101 agcacgtctt gcatgggcaa attaagtttg tactgttgtt ccaggctcag ttgaccgccg
     2161 ttttgagcca gctcccgtct gattgcaccg gcttgcagaa gcaggctacg gctgtcaccc
     2221 ggctgcgctt cgccgagcaa ctgcggccgc tcatttaagc gcctggccgc cagaccgttg
     2281 tagatggcgg aatctaaagt aagactggcg cgcttctcgg ctggagttag atcaacatcg
     2341 cttttgccca ggcgttttgc aacttccggc tctctcaaga gttcttgagt ttccttgatg
     2401 gtcaggggct tcccgctcag ggcgctgttt tcaatcttgt cgaagccttt caatacaggc
     2461 ggctcgccgc tttcagcgac ttgtttgagc aggcgctgcg agagcgggcg cacttcggcc
     2521 ggaccttttg ccagctcatc atctacttgc tttctgacct gagcatcctc tttgtactgc
     2581 ctggcttgct ccggcgtcat ggttgtgatg gctttgagag aatcttccag ttgttgtttg
     2641 tggttgccgc ccctggcatc gctgagggtc tcgtcgaggc tgcgaccgac tttctgggcg
     2701 tcggcaaaag tttctttatt ggccttttca gaaagcatac gcatgatgcg cgcgctctcc
     2761 tccgggttcc cggcaaagcg acctaccgat tcttccacct gtctcttgaa cgcggcacct
     2821 tctgctttct cggccggggt ctgcccgtgc tccgcatttc gcagggtgcg ccaatctttc
     2881 tcgttcagtt tgcccgccgc cgtaagcgcg tccgcgctcg agtggctcga accccacagc
     2941 agcccgtctt tctctgtctt gcccagctcc gatactatgg tttctcggcc gttcaagagg
     3001 gtatcctgcc agcgtatggc atcaccgcgg tttcctcctt tttccaggga ggcttgcagg
     3061 tttcggtaaa agcccagtga cttttgctcg tcggggctga gcttcgagcc gtcttcgccg
     3121 ttatctttca ggcgccgatc cagatcgtag ccccgcttga actcctgtct ttcgtcggcg
     3181 gaagcgtgct tgaccgccag gtcggtattg gccttattgc cgaagaaata gcccgagttg
     3241 gcttccgcca ccgtgctcag gcgtacccgc ccttcctgca gatagtcgcg caaaacggga
     3301 tcaacacggt tgctgggctc tttcagttcg gcttccgtga ggtttgtggc cggggccttc
     3361 tctttgaagt tgcgcagaat gccggcattg aaagcccggt catccttgag catttcccgg
     3421 gcttgctttg cctccggcgt ggtgcctgcc actgcttcaa tgatcaaatc tctgttgccg
     3481 gtgcgagcac ccaggcgtgc cagttggctc agttcttccc ctgataccgg gcggttttcc
     3541 ctggccgcct ttttgtaagt ttctatggca ctgatagtgc tttccggcac gccggcctgg
     3601 cgcagggcct gctccaccga gccgcctttt tcgctgaagc ggctttccat ggtctgaatc
     3661 tgttccgggt tgagcgtgcg gtagagaagc cttatttgcc catctctctt ttgcggatcg
     3721 cgctcagaaa gagctgcttg caggtgggcg gcgtcatcgg ttttgccgtc ctttttatca
     3781 agcagggcaa gggctctgtg ctgatcgccg ccggacaact tttgcaggtc gcggcgcagg
     3841 tctcgctcgg ggcctagctc acttttgtag agtgcttcca gtttcgctct ttccccatca
     3901 cttaggctgg ccagactggt caataccgac ttaccatcgg gattggcgat gcccagggta
     3961 gtcttagaaa gagcgcggtc cagttctctg acactgccac ggacagtgtc gtctgaggtg
     4021 atttgcagtt ccggcaagta cttttctgtg gaggcaggca gggtttgcgt ggcatcggtg
     4081 agggggcggc tgttgccctg acttttgtcg ctctcaggct tgaccgtctc caggttatcg
     4141 ctgggcttga cggcaggctg agtggcttcc agactgaggg cctgccgcga ctgggcaggt
     4201 tgagtgtctg cctcgttttg agaatttgct tcgggttttt cagccatagg tagtaaaaga
     4261 agaaaaatgg gaaggtgcca tgagtattgc tggcggacca tgagaattgc gtgaaagtcg
     4321 aggttttgtg tgttccccaa aacaacagtt gattaactgt tactgccgtg agcatggttt
     4381 gtcgaggcgc cttatactgg cctgaagttt taccaacctt gcggagttgt gcaaaaatga
     4441 atgcggcacg gcttttgcgc gaactgggcg ccagtttctg ggatgatctg acaggtgcct
     4501 tttcgttgag aaactggcaa gattgtctgg ctaagctttc aataacaagt aggaagcaat
     4561 tgatcgagaa tttgctgggt attattccgc aagtggctat cttctatctg gtgctcacgc
     4621 cggtagtggc catgccgctc tacaataaaa tgcttttctt cccgctcaag caacacgcca
     4681 tagactttgc cgagttggcc ggcgttgcca aagtaaacgt gcaaatagtt ggtgagaaca
     4741 gtcgtaagct gagcgcctgg tactttccgg ttgatggcgc caggggggct gtaatcatca
     4801 gtcacggcaa tggcggcaat atctcacatc gcggtcccct gattgagctt cttttgcggc
     4861 agaaaatgtc ggtgttagcc tatgattatc agggctatgg cgagagcgag ggcgagcctt
     4921 ccatcgataa tatctgccgg gacggtctgg ccgcttacga ttatctggtt acgcaaaagg
     4981 gcgtaaaacc agaaaagatt gtgctctatg
//
LOCUS       DAOQNZ010000081         4985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7029_length_4985_cov_3.975254, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000081 DAOQNZ010000000
VERSION     DAOQNZ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7029_length_4985_cov_3.975254"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PKW73_02285"
     CDS             <1..852
                     /locus_tag="PKW73_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNA72138.1"
                     /translation="KRVVELARTSLLLAENNTVEHPQKITDELDQLKEELALAFDGLK
                     EAERSREQLMNSLAHDIRAPMQSIQIALETLAKLEPQTDARGAGRLDTSKLFRQATAG
                     IYEGSELINDLLTLNKLKTAPQKALSDTAAARELEQSEPINWQELLYEAIANQADRSG
                     ARDIGLELEDCPEGNLRLAGHVDSDLFLQMLECAIKVAVDNAPAQTQVIIKLNHKRGL
                     SLSIKDEGQPLGAQYDLPIKLTRPREPGRLKELLALAILEQLAQANQMRLKLSSHGAN
                     EITLSKS"
     gene            complement(894..1571)
                     /locus_tag="PKW73_02290"
     CDS             complement(894..1571)
                     /locus_tag="PKW73_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA72139.1"
                     /translation="MSKVLIVDDDVLLSHSLKLYLEEQNYVVETIGDGEDALHLLRSF
                     PYDAIILDWNLPGMSGETVCLEYRKGGGQAPIIFLTGQNDISFIERGLGVGADDYLTK
                     PFEVRELAARLRSMFRRRRGEFEAELRAFNIVLKPELNLLVSDDTKITLRPKETAVLE
                     YLMRNPNRIFSAQQLLDSVWSADSTATANTVRTWMGLLRQKLAQVGRETLIRTVPGAG
                     YVLDLPD"
     gene            1706..3415
                     /locus_tag="PKW73_02295"
     CDS             1706..3415
                     /locus_tag="PKW73_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase associated domain-containing protein"
                     /protein_id="HNA72140.1"
                     /translation="MSNGALHSHTALSGPVSGSALGQGQLAAEGKLPFSVAVPSQSQE
                     PKEAAAFCQTLQERKEEGPVQSQGNELIDELEAQLKQFENIYRGLAVNEAKPALRHFQ
                     LIVEQTDKRYDTARLNALKEVERAQIILIQASTQKRNLRNSIESYLADLGTAGGEQPL
                     GDEADALKLEEVIEAIYKLLNGRDTHQTHEKLQDFLAGHPHLAMALEQHHKQDKLEKR
                     ILERLSEIENHTLALKQLRISARLMIARSYEKLSYFVQAYNLKKEAGSLTGNLMADST
                     NRVKAAVVDRDANFCAMYQALLAFKEIHGHCDVPRRYKQDPKLAIWTANLRSRRKKGL
                     LPDSRVQALDQVGFHWQSSRSNWASHIEELEKFIKQHGHSFVPMYYPENPELATWVRN
                     LRKDCRRKAVSQSRIDQLTKLGFDFQVLDRKWYERLEELRSFRFRFGHCRVPHRWKEN
                     PTLARWVCHQRQAWRLNQLSLSRLALLEAAGFEWAPAKGRPRKGGGKAKTYSANLNKG
                     GDFMTFYATNQGEASTEIQIQTAETYTAALSGERLSSGAQAASQTAVEYRIGQEAKPW
                     KTT"
     gene            3400..4425
                     /locus_tag="PKW73_02300"
     CDS             3400..4425
                     /locus_tag="PKW73_02300"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA72141.1"
                     /translation="MENHLKSDTTRYERQARLSRETSQEGGRDLESSSPGAGEVTVYG
                     FTNRTPAADGKSFLPLMELEDLDKNTSYYKQTIRFETDSKSALVAETAGDITLLNSEQ
                     YLAEIARSKETTNGKLGIYIPGIRNAPGVAAFNAGQMARSMQATIVVADWASSSPREN
                     EGVVDMVSQTMDDTRAAIKSQPFIDRGIVTLLERLGSGNVDLIAHSRGASLLCNSLST
                     LQSLGMPPVKAATFLHGDKATEKYIEMFPQMYRAANRVNFVVDRNDLALKLSSAARFM
                     VTPVLDKDWRLFPSTANIGSVGLTGAMKERTRPYVSDNYFRVFQDTGFGLLRHTPGLA
                     STISAMR"
     gene            4571..>4985
                     /locus_tag="PKW73_02305"
     CDS             4571..>4985
                     /locus_tag="PKW73_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72142.1"
                     /translation="MDRASTTHHGHKGEVGAAAQNHSLESQSLMYTGAPERFSFPPLK
                     TTQPPIISNRQEANDRSQSGATATDRNQTGQVQTDQSRSQQTQPEKATNGQEPAAGSK
                     PTYLSFATEDLYSSCHKPEASRAECYKYFLENYQKE"
BASE COUNT         1354 a         1423 c         1267 g          941 t
ORIGIN      
        1 aagcgagtgg tagagctggc ccgcacatca ctcttactgg ccgaaaataa tacggtggaa
       61 catccgcaga aaatcacgga tgagttagac cagttgaaag aggagctggc tctggctttt
      121 gacggtctca aagaggcgga acgcagtcga gaacagctca tgaacagcct cgcccatgac
      181 attcgcgccc ccatgcagag tattcagatc gccctggaga ccctggccaa gttagagccg
      241 caaacagatg cgcgaggtgc cgggcggctc gacacgagca agctgttcag gcaggctacg
      301 gcaggtatct atgagggttc tgagctgata aacgacctgc ttaccttgaa caagttgaaa
      361 accgccccgc agaaggcgct ttcagacact gccgccgcac gggagttgga acaatccgag
      421 ccaatcaact ggcaagagct tctctatgaa gctatagcca atcaggccga ccgctcagga
      481 gcgcgtgaca tcggtctgga actggaggac tgccccgaag gcaatctcag actggcaggg
      541 cacgtggata gcgatctgtt cttgcaaatg ctggaatgcg ccatcaaagt ggccgtcgat
      601 aatgcccccg cgcagacgca agtaataatc aagctcaacc acaaacgagg actgtctctc
      661 tccatcaagg acgaaggaca accgttggga gcccagtatg atctaccgat caagctcacc
      721 cgccctcgag agccgggcag gttgaaagag cttctcgctc tggccattct ggaacaactg
      781 gctcaagcaa accaaatgcg gttgaaactg agcagccatg gcgccaatga aatcaccctg
      841 agcaagagct gagtgcaagg ggctttctat atcagctttc tctttcgcct ttatcaatca
      901 ggaaggtcca gtacataacc ggcgccgggc acagttctga tcagagtctc ccgccctacc
      961 tgagccaact tctgccgcaa gagccccatc caggtacgca ctgtattggc tgtggcggtg
     1021 ctatcggccg accagactga atcaagcagt tgctgggcgc tgaagatgcg attaggattc
     1081 cgcataagat actctagaac agctgtttcc ttgggtcgga gagtaatctt cgtatcatcg
     1141 ctgacgagca gattcaattc cggtttcaac actatattga atgcacgcag ttccgcctca
     1201 aactcgccgc ggcgacgcct gaacatactg cgcagccgcg ccgccaattc ccgcacctca
     1261 aatggcttgg tcaaataatc atccgcgccg actcccagac cgcgttctat aaaactaata
     1321 tcgttttgtc cggtaagaaa aattatcggc gcctgtccgc cgcctttccg gtattcaaga
     1381 caaaccgttt caccactcat acccggaagg ttccagtcaa gaatgatggc atcgtaagga
     1441 aaactacgca gcaggtgaag agcatcctcg ccgtcgccga tggtttccac cacataattt
     1501 tgttcctcaa ggtaaagctt gagagagtga cttagaagta cgtcgtcatc aacaatcaaa
     1561 accttggaca tcgccgccta ctttcctttg ggtttagcca ggcaattata caaacgtcga
     1621 ggcggatttg atccagattt tatacgctcg cgcctatcct gcaaaacaca tccccacttg
     1681 atgatttgta ttgagggccc aaacaatgag caatggtgcg ctacattccc atacagccct
     1741 gagcggtccg gtttccggct ctgcactcgg gcaaggtcag cttgccgcag agggcaaact
     1801 tccattctca gttgctgttc caagccagag ccaggagcct aaagaagctg cggcgttctg
     1861 ccagactcta caagaacgga aggaagaagg ccctgtgcaa tcccagggca atgaactgat
     1921 tgatgagctg gaagcgcaac taaagcagtt cgagaacatc tatcgcgggc tggcggtgaa
     1981 cgaagcgaaa ccggcattgc gccattttca gctaatcgtt gaacaaaccg acaagcgcta
     2041 cgacacagcc cgcctcaacg ccctcaaaga agttgaaaga gcccagataa tcctcatcca
     2101 ggcctcaacc cagaagcgca acctgaggaa cagcatcgaa agctacctgg ctgatctcgg
     2161 cactgcaggc ggagaacaac cgctcggtga tgaggcggac gcactcaaac tcgaggaagt
     2221 catcgaagca atttataaac ttctgaacgg ccgggacacc caccagactc acgagaaact
     2281 gcaagacttc ctggccggcc acccacattt ggctatggct cttgaacagc accacaaaca
     2341 agacaaactg gagaagcgca ttctcgaaag gctctcggaa atcgagaacc acaccctggc
     2401 cctcaagcag ctacgaattt cagcaagact gatgatcgcc aggagctatg aaaagctcag
     2461 ctatttcgtg caagcttata acctcaagaa agaggccggc agcctcaccg gcaatctcat
     2521 ggcagatagc acgaaccgcg tcaaagcggc agtcgtagac cgcgatgcca acttctgcgc
     2581 catgtatcaa gctcttctgg ccttcaaaga aattcacgga cactgcgatg ttcccagaag
     2641 atacaagcaa gaccccaagc tggccatatg gaccgccaat ttgagaagcc gccgcaagaa
     2701 aggtttgctg cctgactcac gagtgcaggc cctggatcaa gtcggctttc actggcaatc
     2761 gagccgcagc aactgggcct cccacattga agaactggag aaattcatca aacagcacgg
     2821 tcatagtttt gtgcccatgt attatccgga gaatcccgaa ctggccacct gggtacgcaa
     2881 cttgcgcaaa gactgccgcc gtaaagcggt gtcccagagc cgcatcgacc aactgactaa
     2941 gctgggcttc gacttccagg tcttagaccg caaatggtac gaaagattgg aagagctgcg
     3001 cagttttcgc tttcgtttcg gacactgccg ggtgccccat cgctggaaag agaaccccac
     3061 tttggctcgc tgggtttgcc atcagcgcca ggcctggcgc ctcaatcaac tttccctgag
     3121 cagattggcc ttgcttgaag cagccggctt cgaatgggca ccggccaagg gtcgcccccg
     3181 caagggcgga ggcaaagcga aaacctattc tgccaacctg aacaaaggcg gcgactttat
     3241 gaccttctac gccaccaacc agggagaggc aagcaccgaa atacaaattc aaaccgcaga
     3301 aacctacact gccgctcttt caggtgagcg tctttcgtcc ggcgctcaag cggcaagtca
     3361 aacagccgtc gaataccgca tcgggcagga agcgaagcca tggaaaacca cctgaagtcg
     3421 gacactacca ggtatgaacg gcaggcgcga ctctcccggg aaacttcgca ggaaggggga
     3481 agggaccttg aatcttccag ccccggagcc ggcgaggtaa ctgtgtatgg cttcaccaac
     3541 cgcactccgg cagccgacgg taaaagtttc ttacccctta tggaactgga ggatctggat
     3601 aagaacacca gctactacaa acagaccatt cgcttcgaaa cagacagcaa atcggctctg
     3661 gtagcggaaa ctgcaggcga tataacgcta ctgaatagtg agcagtacct cgctgaaatc
     3721 gctcgctcaa aagaaaccac caacggcaag ctcggcatct acattcccgg aattcgcaat
     3781 gccccgggtg tagccgcttt caatgccggg caaatggctc gttcaatgca ggccaccatt
     3841 gtggtagccg actgggcgag cagcagcccg agagagaatg aaggagtggt ggatatggtt
     3901 tcacagacta tggacgacac cagggccgcc atcaagagcc aaccttttat cgacagaggt
     3961 atcgtcactc tcctggagcg gctgggcagc ggaaatgtgg atctgatcgc ccacagtcgc
     4021 ggcgcctcgc tgctgtgcaa ctctctatcc accttgcaaa gcctgggtat gccgccggtc
     4081 aaagccgcca cctttttaca cggagacaaa gctacagaaa aatatatcga gatgtttccg
     4141 caaatgtacc gagcggcaaa ccgagttaac tttgtcgtcg acagaaacga cctagcgctg
     4201 aaactttcca gcgcagcacg tttcatggtc acgcccgtgc tggacaaaga ctggcgactc
     4261 tttccgtcaa cagcaaatat cggctcggtt ggtctgactg gtgctatgaa agagcgcact
     4321 cgcccctacg tgtccgacaa ttacttccgc gtctttcagg acaccggctt cggtcttctc
     4381 aggcatacac caggtctggc cagcactatc agcgccatgc gataagcatc ggcgcaccga
     4441 tgtaggccgg gtagatcgag taaaagtttg agtagcagat agaacaatgg tttgagcggc
     4501 agacagaaca atggtttgag cagcacatag agcaaaagtt tgagcagcag acaaaacaat
     4561 ggtttgaaaa atggacagag caagcacaac acaccacggg cacaaaggcg aagtcggcgc
     4621 cgccgctcaa aaccattccc tggagagcca gtcactgatg tataccggcg cccctgagag
     4681 attctctttt ccgccgctga aaacgacaca accgcccatc atttcgaacc gccaggaagc
     4741 aaacgaccgg tcccaatccg gagcgacagc aacagatcga aaccaaacag ggcaagttca
     4801 aaccgaccag tcacgctctc agcaaacaca gccggaaaag gctacaaacg gacaggagcc
     4861 cgctgccggc agcaaaccaa cttacctgag cttcgccacg gaagatctat acagcagctg
     4921 ccacaaaccg gaagcatccc gggcggaatg ctacaaatac ttccttgaga attaccagaa
     4981 ggagc
//
LOCUS       DAOQNZ010000082         4982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7040_length_4982_cov_3.502334, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000082 DAOQNZ010000000
VERSION     DAOQNZ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7040_length_4982_cov_3.502334"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKW73_02310"
     CDS             <1..235
                     /locus_tag="PKW73_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72143.1"
                     /translation="YCRHSQPVNFVSDTSEFDYIAQSRTPVLCCPCDFSTFDPNDNGR
                     VLGGPAYRPLRRMTVAFDGRCYKITGIEQGEIA"
     gene            527..1924
                     /locus_tag="PKW73_02315"
     CDS             527..1924
                     /locus_tag="PKW73_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72144.1"
                     /translation="MSMKNFQELTNGQDIRDAGNFDTWAHRASNDASDFFRSQSRNHG
                     IDRESQHLLTSLEITPIFTNHNSFQAGRGAFQPDRFMDGLRRLDPDLSDVVTPCPPGF
                     DRARWYGGDKSPEYTLARGMANSLNELSSIPDAASRKTFVERVLQNQVPELEKQGAKV
                     HGIQGERIKIDFGNGRGPQWIDTVRDLYGSPEVQLLNVTALMRNQEGNGRVTMRPQVE
                     PGRFPIDNTGDNGNYMPKYPVDQALPPCPPGFDATKWNNGHDTIKYAVGRDLARVFDQ
                     LKAIPDEAGQKKFAENFLKSQVPAMEARGGKVLAIKDEKVLLDAGEGSGPLWIDVVKD
                     IGGKNPEVQWTVIGKGNPGQTEAPEFAPCPPGFDPVKWDNGHDTPKYFVGHLVADMLQ
                     QDRLNPDREGQKARVEKFLKEHAPLMQAKGIKVYDIIGEKIMMDVGDGTGKHWVDTVA
                     DVGGKNPQASWQVEA"
     gene            complement(1950..3413)
                     /locus_tag="PKW73_02320"
     CDS             complement(1950..3413)
                     /locus_tag="PKW73_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA72145.1"
                     /translation="MVDRGAYLKLSIAQKGLIIAAVPLVWGLVYLSVLGVLLERAEAT
                     ARRESRARAIVSEASQAQRDLFDAGSAIVAWNYMRTSALERRLDASIAAITQRVEKIK
                     ELMKGDSSRADKLRTVEDRARQIVSVLQWHKAQIKNSSNSLYSFEFTRFRKQIEDAFK
                     TYVDELHILTTEERAIEEHTLQREEARRQELQLYILAGVALNLALSLGVAVYFSRDIA
                     FRLNLLKKNAERVVKRQSLLPTISGSDEVAEVDAAFRRMASDLNRAEAAKRDFVAMVS
                     HDLRSPLSAIQGALELVNADVYGEISAKGKSRVRDAEYECVRLLSLINELLDIEKIEA
                     GMMEMNKETADLAELVVRGSESVKLLAEAKNVRIEVFGAHALAPVDSERLVQVFVNLF
                     SNAVKYSPQGGTISVNIEEKDGRATIKIQDQGPGIPKQLQESVFDRFKQAHGGGSGTS
                     GLGLAICKAIVEAHDGKIGVVSEVGNGSLFWLQLPTR"
     gene            3474..4196
                     /locus_tag="PKW73_02325"
     CDS             3474..4196
                     /locus_tag="PKW73_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA72146.1"
                     /translation="MDKSSESTIAMPRILFAEDEQKLANLVIDFLTAEDYEIDLATSG
                     PAALSRLESDSYDALILDVGLPGLSGFEVCKIYREKGGTAPIIFLTGQNTVADKERGL
                     DLGADDYLTKPFHMRELSARLRAITRRSRTLVSPRLEIGDLSLDSATHTVTKGGTPLQ
                     LMPREYALLEFFMRHPNRTFTQNELLDEVWSKDSEASPDTVRVHITKLRAKIDSPDKG
                     EESLIKTVHRVGYRFEKSVTSD"
     gene            complement(4197..>4982)
                     /locus_tag="PKW73_02330"
     CDS             complement(4197..>4982)
                     /locus_tag="PKW73_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72147.1"
                     /translation="GGVLFAAGGVGSAAAPLARAGRSGYAALRGLAGEADDMAAASTQ
                     KALGGAGEDVLSVRSPGSATDGALKNGIKSPATVEDVPQSPGPVNPESGGGRSRLIGE
                     GDEGKVYDAGKFTGADGRSTESVWKVYEQNALNNSPESVVRLTHELESMGIRVPRVLE
                     TGKTADGHFAIRFEKIGPDGDSLKDDLRVGALSLADRQEARRQYDGIVEKLAANHVYG
                     DLQLKNFIWNKETRELVWVDPGKLKAGDARALSANYSNAIYPQ"
BASE COUNT         1237 a         1441 c         1259 g         1045 t
ORIGIN      
        1 atattgccgc cacagccagc cagtgaattt cgtgagtgat accagcgaat ttgactacat
       61 agcccagtcc agaactccag tgctgtgctg tccatgcgat ttcagcacct tcgaccccaa
      121 cgacaatgga cgagtgttag gcggaccggc ctaccggcca ctgcgccgga tgacagtcgc
      181 ctttgacggt agatgttaca aaatcaccgg aatagagcaa ggcgagatcg cctgagcctc
      241 ctgcggacaa cagggcgaat aggattacca agacagtctt ggaatcgatg agcgtatacg
      301 actggcgaaa aacgcccacc agtgccgcaa ctcgatccaa cgaaatcaaa ccttaaccaa
      361 cccaatccaa tccaattcga gcgtgttaac aactcggaaa caacttcccg gttgccgccg
      421 cctaatatga acgcagtcgg aggcggggcg acgatcgcca tctcccccaa ggtcgcccac
      481 tccgacaaaa ctgagttttt tcaccccacg ctcaggagac caggaaatga gtatgaaaaa
      541 cttccaggaa cttactaacg gtcaagacat acgcgatgcc ggtaactttg atacctgggc
      601 ccaccgggcc agtaacgatg cctccgactt cttccgcagc cagagccgca accacggcat
      661 cgacagagaa tcgcagcatc tccttaccag ccttgaaatc acacctatct ttactaatca
      721 caattctttc caggccggac gcggcgcctt ccagccggac cgctttatgg acggtctgag
      781 acgccttgat cccgatcttt ccgacgttgt taccccatgc ccgcccggct tcgacagagc
      841 acgctggtac ggcggcgaca aatctcctga gtatactctg gctcgcggta tggcaaacag
      901 tttgaacgaa ctgagcagca ttcctgatgc tgcctcacgc aaaacctttg tggaaagggt
      961 tctgcaaaat caagtgcccg agctggaaaa gcagggcgct aaggtgcacg gcatccaggg
     1021 cgaacgcatc aagattgact tcggcaatgg acgcggaccg cagtggatag acacggtgcg
     1081 cgacctgtac ggctcaccgg aagtacagtt gttgaacgtc acggctctga tgcgcaacca
     1141 ggaaggcaac ggaagagtga ccatgcggcc gcaggtggag ccgggacgct tccccatcga
     1201 taataccggc gacaacggta attacatgcc caagtatccg gtggatcagg cgctaccgcc
     1261 ctgccctccc ggtttcgatg cgacaaaatg gaacaacggc catgacacta tcaagtatgc
     1321 cgtcggccgt gatctggcca gggtcttcga tcaattaaag gctattcctg atgaagccgg
     1381 acagaagaaa tttgccgaaa acttccttaa gagccaggta ccggccatgg aagcacgagg
     1441 cggcaaggta ctggcaatta aggacgagaa ggttctgctg gatgccggcg aaggctccgg
     1501 acctctctgg atagatgtag tcaaagacat cggcggcaag aacccggaag tacagtggac
     1561 tgtgattggc aaaggcaacc caggccagac cgaagcaccg gaattcgccc cctgcccacc
     1621 cggtttcgac ccggtcaaat gggacaacgg acatgatacg cccaaatatt ttgttgggca
     1681 tctggtagcc gacatgctgc aacaagacag actcaatcca gatagagaag gacagaaggc
     1741 ccgcgtagag aagttcctga aagagcatgc accattgatg caggctaaag gaataaaggt
     1801 ttacgacatc atcggtgaaa agatcatgat ggacgtaggt gacggtaccg gcaagcactg
     1861 ggtcgacacc gtagccgacg tcggcggcaa aaatccgcaa gcaagctggc aggtagaagc
     1921 ataaagaaaa gagtttattt cgtgtggggt taacgggtgg gcaactggag ccagaacagg
     1981 cttccgttgc ccacttcact tactacaccg attttgccgt cgtgggcttc tacaattgct
     2041 ttgcaaatag ccaggcccag accgcttgta ccactgcctc caccatgggc ctgcttgaag
     2101 cgatcgaaga ctgactcctg caattgcttt ggaatgcccg gcccctgatc ttgaattttg
     2161 atagtggcgc gtccgtcttt ttcctcgatg ttgacgctta tggtgccgcc ctgaggcgaa
     2221 tacttgacgg cattggagaa gagattgacg aagacttgca ccagtcgctc gctatcgaca
     2281 ggcgccaggg catgagcacc aaatacctca attcttacat tcttggcctc ggccagaagt
     2341 tttaccgatt cactgccgcg caccaccagc tcagccagat cggcagtttc cttgttcatt
     2401 tccatcatgc cggcttcaat tttttcgata tcgagaagct cattgatgag cgacagcaag
     2461 cgcacacatt cgtattcggc atctctcaca cggcttttgc cctttgcgga tatttcgcca
     2521 tagacatcgg catttacaag ctccagagcg ccctgaatgg cagacaacgg actgcgcaga
     2581 tcatgactga ccatggccac aaaatcgcgc ttagccgcct cagctcggtt taaatcggaa
     2641 gccatgcggc gaaaggcggc gtccacctcg gctacttcat cagagccgga gattgtcggc
     2701 aagagagatt gtcgcttcac tacccgctct gcattctttt tgagaaggtt gagacgaaag
     2761 gcaatatcgc gactgaaata aacggcgacg cccagtgaga gcgccaggtt gagcgctact
     2821 ccagccaaaa tatagagctg caattcctgt ctgcgcgcct cttccctctg tagcgtgtgt
     2881 tcttctatgg ctctttcttc cgtggtgaga atgtgcaatt catcaacata agttttgaaa
     2941 gcatcttcga tctgcttgcg aaagcgggtg aactcgaagg aataaaggct attggaagaa
     3001 ttcttgatct gggctttgtg ccactggagc acgcttacaa tctggcgagc ccgatcttcc
     3061 accgtgcgca gtttatcggc acggctgctg tcccccttca taagctcttt aattttctcg
     3121 actcgctggg ttatagcagc gatgcttgca tcaaggcgtc tctccagggc gctggtacgc
     3181 atataattcc aggcaacaat ggcgctgccg gcatcaaaga gatcgcgctg agcctggctg
     3241 gcttccgaga caatggcccg ggcccgtgat tcgcgtctgg ccgtggcttc cgcccgctcc
     3301 aggagcacgc ccaggacgct gaggtagact aacccccaga ccaggggtac agcggcgata
     3361 atcaagcctt tttgagcaat gcttagcttc agataggcac ctctgtcaac cattgcctcc
     3421 attctacccc tagcaggctg ctaccggagc aagctaaaat agaacctcag tctgtggata
     3481 aaagttccga aagtaccatt gccatgccca gaatattgtt tgccgaagat gagcagaaac
     3541 tggccaacct ggtcatagac tttctcacgg ctgaagacta cgaaatcgac ctggccacta
     3601 gcgggccggc cgctctcagt cgcctggaaa gcgacagcta tgatgcgctc attctggacg
     3661 taggcttgcc cgggctgagt ggttttgagg tatgcaagat atatcgagag aaagggggca
     3721 ctgcgccgat aatttttctc accggtcaaa acacagtagc cgacaaagaa cgtgggctcg
     3781 atctgggtgc cgacgactat ctcaccaaac ctttccacat gcgcgaactt tcagccagac
     3841 taagagccat caccagaaga tcgcgcaccc ttgtttcacc acgccttgag ataggcgacc
     3901 tcagcctcga tagcgccacc cataccgtca cgaaaggcgg cacgcccttg caactgatgc
     3961 cgcgggagta tgcccttttg gaattcttta tgcgtcatcc aaaccgcacc ttcacccaga
     4021 atgagctact tgacgaagtc tggtcaaaag atagcgaagc ctcaccagat acggtgcggg
     4081 tgcacattac aaaactgcgg gccaagatag acagtcccga caagggcgaa gaatcgctca
     4141 tcaagaccgt gcaccgggtc ggttacagat ttgagaagtc agtcacatcg gactagctat
     4201 tgcggatata tagcgttgct ataattggca ctcagcgctc tggcgtctcc ggctttaagc
     4261 ttgccgggat caacccagac cagttctcga gtctccttgt tccagataaa gtttttcagc
     4321 tgcaagtctc catacacgtg attagcagct aatttctcta caatcccatc gtactgcctg
     4381 cgagcttcct gccggtcagc cagggaaaga gcaccgactc gcaaatcgtc cttcaaactg
     4441 tcgccatccg gtccgatctt ttcaaatcgg atggcaaagt gaccgtcggc cgtctttccg
     4501 gtctcaagca ctcgcggcac acgaatgccc atactctcta actcatgagt taacctgacc
     4561 accgactcag gcgagttatt cagggcattc tgctcataaa ccttccaaac agactcagtg
     4621 cttcgaccgt ctgcaccggt aaatttaccc gcatcgtaaa ctttgccttc atcgccctca
     4681 cctatcaaac gactgcgacc acctccagac tcagggttca caggacccgg agactgaggc
     4741 acatcttcga cggtagccgg cgattttata ccattcttga gagcgccatc cgtcgccgag
     4801 ccagggctcc ggaccgacaa aacatcctct ccagctccac ctagcgcctt ctgagtggaa
     4861 gcggccgcca tgtcatcggc ttcaccggcc aatccacgca gagccgcata gccggagcga
     4921 ccagcccgag ccagtggtgc cgccgcgctg cctacaccac cggcagcaaa gagcacaccc
     4981 cc
//
LOCUS       DAOQNZ010000083         4981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7045_length_4981_cov_3.374340, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000083 DAOQNZ010000000
VERSION     DAOQNZ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7045_length_4981_cov_3.374340"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKW73_02335"
     CDS             <1..387
                     /locus_tag="PKW73_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72148.1"
                     /translation="ILIQDHMEPALKPDGSMNPLRPVYAQAVKFISLGKESDPFIADL
                     TGAFQVLDGTEKMNAKTFFGCLPQMRKPFNVARDVTVLKLYMLEPDRGDAYCDWNLIV
                     NAAARKLTIKMELDRFKVGLVRSLSQ"
     gene            485..799
                     /locus_tag="PKW73_02340"
     CDS             485..799
                     /locus_tag="PKW73_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HNA72149.1"
                     /translation="MSWIILLVAGLFEVGWAVGFKLEAGPSKPLLLFLTVVSLILSMA
                     LLSFSMRELPLGTAYAVWTGIGVLGTFLVGVLCFHESVGMLRLASVMLILVGLLGLKL
                     AK"
     gene            complement(804..4463)
                     /locus_tag="PKW73_02345"
     CDS             complement(804..4463)
                     /locus_tag="PKW73_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNA72150.1"
                     /translation="MEKSGKSNSLEQAFSEPAAESFTEAGFRFFVDRGGTFTDIIGVG
                     PDQHVYVHKLLSQNPNSLTDPTRAGIEAILTCAGAAAHGRSDEIRVGTTVATNALLTR
                     SGARLVLLTNKGLKDALSIGYQARDDIFALEIRKATPLYFEVMEIDCRMTADGQVSKP
                     VDEEEVLNLLRQLKEREPDAVLAVVFMHSYLNGEQEDEVASLATSLGFTNLSLSHKVS
                     PLIKYVPRGDTTCVDAYLNPPLKAYTERLAAQLEGSLSFMKSDGGLTTMDEFSGKDAL
                     LSGPAGGVVGAVKFATARGEAQLIAFDMGGTSTDVSYFAGEFEKQYETIIASVRVRSP
                     MMAVHTVAAGGGSILAFDGARLTAGPESAGSYPGPSSYGHGGPLTITDANLILGRISV
                     AHFPKTFGKKQDAALDTVTPRKLFQEMGELVAAKTGLKKYLQPEVLAYGFLTVAVEKM
                     CRAIARVSTEQGHDVSQATLVAFGGAGGQHACLIAERLGIKKIIVSPLAGVLSAYGIG
                     VTAHSASASCSWRQPLATLPSPSLQTRFEKLRESAQRKLKTKVGATDRLVFLRYQGSD
                     CSTAVKLDESPSIEQVRKTFEEKHEKIFGFHLPAAEIVIEDLAVELKEESRPLDTGAL
                     TFISYDDVPAACQKLYAGGRFHDVTPLTVKDLHAGLTITGPALLAGATATTVIEPGWR
                     ALVEADGGLTIIREEGERSAADPASHSQADPVLLELFNNIFMAIAEEMGTTLQHVSHS
                     VNIKERLDFSCAIFDGEGRLIANAPHMPVHLGSMGEAVVALMKARVEDLQEGQVYVSN
                     NPYNGGTHLPDITAISPLFAGESKPLFFVASRGHHADIGGITPGSMPPLSTTIEEEGA
                     LLDNLLIMQDRVLLEDSVRRAFLDNRYPARNIEQTMSDLKAQIAANVKGIQALKKLMR
                     ERGLQTVQQYMQHIRANAAMAIREILQELEEGAGTATMDDGSIIAVQIKIDKANKQAV
                     IDFTGTSPQTMNNFNTPRAVVRAAVLYVFRTLIKHEIPLNDGCSEPLNIIIPQGSLLS
                     PSYPRAVVAGNVETSQIIVDLLYQCLGRLANSQGTMNNLTFGNERYQYYETICGGSGA
                     GPGFAGSSAVQTHMTNSRLTDPEILETRYPVRLEEFSIRTGSGGAGLFPGGDGACRTL
                     RFLENMEVSILSGRRRTRPEGLRGGGGALSGRTTWIKAGGESSLLSSTDHVLVQAGDC
                     IRIETPGGGGYGARE"
     gene            complement(4498..4794)
                     /locus_tag="PKW73_02350"
     CDS             complement(4498..4794)
                     /locus_tag="PKW73_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72151.1"
                     /translation="MHISQTSPARAKALAIEQPSAPPSTAPERKRKMPSARTRRRSRA
                     QALTEYSTLIAFVSVLIALAFSFTEGKVAPAVSKAFHETAKQLDALSTAALGAS"
BASE COUNT          986 a         1325 c         1438 g         1232 t
ORIGIN      
        1 atactgatcc aggatcatat ggaaccggct cttaaaccgg atggctccat gaatccattg
       61 cgaccggttt atgcccaggc cgtcaagttt atctctctgg gcaaggagag cgaccctttc
      121 atcgctgatc tgacgggcgc ctttcaggta ttggacggta ctgagaagat gaatgccaag
      181 actttcttcg gttgtctgcc gcagatgcgc aaaccattca atgtggccag ggatgttacg
      241 gttttgaagt tgtacatgct ggaaccggat cgcggcgatg cctattgcga ctggaatttg
      301 attgtcaatg cagcggccag gaagctcacc ataaaaatgg aattggaccg atttaaagtt
      361 ggtctggtgc gttctctttc acaatagttt gccgtccggg ctttagtatt gctattatca
      421 aagtactggg gacgaccccg gtctgagttt catgcgggac ggcccgatgc gcaagcgagg
      481 ccttatgtcc tggattattt tattggtggc tggtttattt gaagtcggtt gggctgtggg
      541 cttcaagctg gaggcgggac cgtccaaacc gcttctgctt ttcctgacgg tggtttccct
      601 gatcctgagc atggcccttc tttccttctc catgcgagaa ctgcctctgg gcacggctta
      661 tgccgtctgg accggcatcg gtgtgctcgg cacattcctg gttggggtgc tctgcttcca
      721 tgagtcggtc ggcatgctgc gcctggcttc cgtgatgctt atccttgtgg ggcttctagg
      781 tttgaagctg gcgaaatgag ccctcattcc cttgcgccgt agccgccgcc gcccggagtt
      841 tcaattctaa tgcagtcccc tgcctgcacc agcacatggt cggtgcttga aagcaagctg
      901 gactcaccgc cggctttgat ccaggtagtg cgaccactga gagcgccgcc accgcccctt
      961 agcccttccg gtctggttct acgtcgtcct gagaggatgg agacttccat gttctccaga
     1021 aagcgcagcg tgcggcaagc accgtcgcca ccggggaaga gtccggctcc gccgctgcct
     1081 gtgcggatgg aaaactcttc caaccttacc ggatagcgcg tttccagtat ctccggatcg
     1141 gtgaggcggg aattagtcat atgggtctgc actgccgaag agccggcaaa gcccggtccc
     1201 gctccggagc caccgcagat ggtttcgtaa tactgatagc gttcgttgcc gaaggtgaga
     1261 ttattcattg tgccctggct gttggccagt cgccccaggc actggtagag aaggtcgaca
     1321 ataatttgag aggtttccac attgccggcc accaccgccc gcgggtagct gggagagagc
     1381 agagagcctt gcggaataat gatattgaga ggttcgctgc agccatcatt gagtgggatt
     1441 tcgtgtttga tgagggtgcg aaagacgtag agcacggcgg ccctgaccac ggcccttggg
     1501 gtgttgaagt tgttcatcgt ttgcgggctt gtgccggtaa agtctatgac tgcttgctta
     1561 ttcgccttat caatcttgat ttgtacggcg atgatgctgc catcatccat agtggcagtg
     1621 ccggctccct cttccagttc ctgtaagatc tctcttatgg ccattgccgc attggctctg
     1681 atgtgctgca tatactgctg caccgtttgc agcccgcgtt cccgcatcaa tttcttgaga
     1741 gcttgtatgc ccttcacgtt ggccgcgatt tgagccttca agtcgctcat ggtttgttca
     1801 atattgcgcg ccggatagcg gttgtccagg aaggcccggc gcacggagtc ttccaggagc
     1861 accctgtctt gcattatgag caagttgtcg aggagcgctc cctcctcttc gatggtggta
     1921 gagaggggcg gcatggagcc cggggtgatg ccgccgatat cggcatggtg cccgcgggat
     1981 gccacgaaga aaagcggttt actctcaccg gcgaacagcg gactgatggc ggttatatcc
     2041 ggcagatggg taccgccgtt gtaaggattg ttggaaacat agacctgccc ctcttgcagg
     2101 tcttctaccc gggctttcat tagcgccacc accgcttcgc ccatggaacc aaggtgtacc
     2161 ggcatatgcg gtgcattggc tatcaatctg ccttcaccgt caaagatggc gcaggaaaag
     2221 tcgagtcttt ctttgatgtt gacggaatga ctgacatgtt gcagagtggt gcccatctcc
     2281 tcggcaatgg ccatgaagat gttgttgaaa agttccagta gtacgggatc ggcctggcta
     2341 tgtgaggccg ggtcggcggc gcttctctcc ccctcttccc ggatgatggt cagtcctccg
     2401 tcggcttcca ccagggctcg ccagcccggc tctatgacgg tggtggccgt ggctccggcc
     2461 aggagagccg gaccggttat ggtcaggccg gcatgcagat ctttcaccgt cagtggtgtc
     2521 acatcgtgaa agcggcctcc ggcatagagt ttctggcaag cagccggtac atcgtcgtag
     2581 gaaataaatg taagggcgcc ggtatcgagg gggcggcttt cttccttcag ttcgacggct
     2641 agatcttcga ttacgatttc ggctgccggc agatgaaagc cgaagatctt ctcgtgcttt
     2701 tcttcgaagg tttttctgac ttgctcaatg gagggagatt catccagttt gacggcggtg
     2761 ctgcaatctg agccctggta gcgcagaaaa accaggcggt cggtggcgcc cacttttgtc
     2821 ttcaatttcc tttgcgcgga ctctctcagt ttctcaaagc gagtctgtaa agacgggctt
     2881 ggcagcgtag ccaggggctg tcgccaggag caagaagcgg aggcgctgtg ggcggtgaca
     2941 ccgatgccgt aagcggagag cactcccgcc agggggctta cgattatctt cttgatgccc
     3001 aggcgctcgg caatcaagca agcgtgctga ccgccggcgc cgccaaaagc caccagggtg
     3061 gcctgactca cgtcatgccc ctgttccgtg gaaactctgg ctatggcccg gcacatcttc
     3121 tccacggcca cggtcaaaaa tccgtaggcc agcacttccg gctgcaggta ctttttcaga
     3181 ccggttttgg ctgccaccag ttctcccatt tcctggaaga gtttcctcgg tgtcaccgta
     3241 tccagtgcag catcttgttt cttgccgaag gtcttgggaa agtgggcgac ggaaatgcga
     3301 ccaaggatca aattggcatc ggtgatggtg aggggaccgc catgtccgta agaggacgga
     3361 cccggataac tgccggcgct ttccggaccg gctgtcagcc tggcgccgtc aaaagccagt
     3421 atggaaccgc ctccggctgc taccgtgtgt acggccatca tggggctgcg cacgcgcacc
     3481 gacgcaataa tggtttcgta ctgcttctcg aattcgcctg cgaagtaaga aacgtcggtg
     3541 gaagtgcctc ccatatcaaa ggcgatcaat tgcgcttccc ccctggcggt agcgaatttg
     3601 accgctccca ctacaccacc ggccggtccg gaaaggagtg cgtccttgcc gctaaattcg
     3661 tccatggtcg tgaggccgcc gtcggatttc ataaaggaga gactgccttc cagttgcgcg
     3721 gccaggcgct ctgtgtaggc tttcaggggc ggatttagat aggcatccac gcaggtggtg
     3781 tcaccacggg ggacgtattt gatcaggggg ctgaccttat gggaaagaga caggttggta
     3841 aatcccaggc tggtggctaa gcttgctacc tcgtcctctt gctctccgtt caaatagctg
     3901 tgcatgaata caacagccag tacggcatca ggctctcttt ctttcagttg cctgagcaag
     3961 ttcagcactt cctcttcatc cacaggtttg ctcacctgcc cgtcggcggt catgcggcaa
     4021 tctatttcca tcacttcgaa atagagagga gtagctttcc ttatttccag ggcgaatata
     4081 tcgtctcggg cttgatagcc gatggagagg gcatctttca aacctttgtt agtcaaaagt
     4141 accagtcttg ccccgcttct ggtcaagagt gcattggtgg caactgtggt gcctaccctg
     4201 atttcgtccg atcgcccgtg ggcagccgct ccggcgcaag tcaggatggc ttcgatgccg
     4261 gctctggtgg gatcggtgag actgtttgga ttttgagaaa gcagtttgtg cacgtaaaca
     4321 tgctgatcag gcccgacacc gatgatgtcc gtgaaggtgc cgcccctgtc tacgaaaaaa
     4381 cgaaaaccgg cttccgtaaa gctttcggca gccggctctg agaaggcttg ctctaaagaa
     4441 ttgctctttc cactcttttc catcgagtgt ctattctaga gcaagaggca agaatattca
     4501 ggatgcacct aaggcggcgg tggagagtgc gtccaactgt tttgcggtct cgtggaaggc
     4561 tttggacacc gctggtgcca cttttccttc ggtgaaggag aaggccagcg ctattaagac
     4621 cgatacgaag gcaataaggg ttgaatattc agtgagagct tgtgcccgac tgcgtcttct
     4681 cgtcctggcg gacggcattt ttcttttgcg ttccggtgcc gtcgatggcg gtgccgatgg
     4741 ttgctctatg gccagagcct ttgcccgagc agggcttgtt tgtgaaatgt gcatggcaaa
     4801 accagttaag cgagtagctg agctaaatag ctcaatgagt agcataatgc ggacgccaga
     4861 tgctaagcat atctgaaata gcttggcaag tcttagtaaa tttaaaaatt ccccccgcct
     4921 atattacagc gattccgcag aagaactacc caagccgtcc ctgacagcag tttcaatact
     4981 t
//
LOCUS       DAOQNZ010000084         4961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7098_length_4961_cov_4.617203, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000084 DAOQNZ010000000
VERSION     DAOQNZ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7098_length_4961_cov_4.617203"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKW73_02355"
     CDS             complement(<1..439)
                     /locus_tag="PKW73_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72152.1"
                     /translation="MNGMSDQTNSGATVVSDADLALSAYHALDDLRYLDQVDRVLARH
                     GDDVDVLYPDPAELADQGADLTVLYAAMQSGMSPAAFGSALAAGVDVSMLSRALSLGL
                     EPGAVEDLVMTYGAELGGHIANYVESKEDEAAYPDPVDRVMARL"
     gene            complement(436..1224)
                     /locus_tag="PKW73_02360"
     CDS             complement(436..1224)
                     /locus_tag="PKW73_02360"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNA72153.1"
                     /translation="MKTSPHLSELSSGGFFAQAKFWMKRYLLWPLFPASRPRLIALLG
                     GPGAGKGTVAGQLAPALGLSHVSTGALIRKEIAAQTDFGKQVQALVEKGLLIPDQMAL
                     TILKRALLAPENARGAILDGFPRSRAQAVLLDELLASWGLSLEKIVWLELSEADLVER
                     LSLRRTCTNQSCGRSYHLKFEPPKNAGICDACSSPLFQRKDDAPETIRERLRTYSEES
                     TPLRSYYQGTKGGVLVFLNPTNAMTREQVLAQVTNAISSKGERV"
     gene            1833..3683
                     /gene="pepF"
                     /locus_tag="PKW73_02365"
     CDS             1833..3683
                     /gene="pepF"
                     /locus_tag="PKW73_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNA72154.1"
                     /translation="MTTTTVMGALPARADVPVELTWDLSKIFATEADWNATFASTEAL
                     VAKLPEFKGKLKRSGKQLLAFLTERDKAAELLDKLYVYASMKADEDTANTANQEREEK
                     IRGLYTKFGAAVSWSTPELLSMKSERLASFIAKTPALNLWKQQFDELNRERAHVRSPE
                     VEELLAQQAMVAQGPQTIFGMFDNADIQFPKVKDAEGNEHQLTHGSYGVFLHSRDSVL
                     RENAYRGMMETFEKSRNTVAAIYSAQVQANIFKARARRYGSSLEAALGPDNVPLSVYK
                     SLIETAHAYLPVLHRLVALKARELAKQGTLPSGKIRLWDLYAPVVGSVDYKVDYTTAQ
                     GIILDAVKPLGTEYVDNLRTGFASRWVDVVENKGKRSGAYSGGAFGTPPYMLMNWKDK
                     LEQMFTLAHEAGHSMHSFFSRRGQPFQYANYTIFVAEVASTCNEALLAHHLLSTESDP
                     AVKRYLIDHQLDAFRATFFRQTLFAEFEMLAHEAAERGEALTADKLSEIFLDLNKKYY
                     GDCVADDDPVKIEWARIPHFYNSFYVYKYATGISAATALSAQILKEGQPAVDRYLKFL
                     SGGCSDYSINLLKGAGVDLSSPDAIKSALDVFTAYLDQLEELIKADEAKA"
     gene            4625..>4961
                     /locus_tag="PKW73_02370"
     CDS             4625..>4961
                     /locus_tag="PKW73_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72155.1"
                     /translation="MNTQQVKPSATVRHVQRQLEVGFNYAIIGCAVTYFDHRITEEEI
                     AETVLHFQGRNITAVPVYEEGCLDFFFLFPADASHLEHPRRFPSANTQVTHFMIVTPD
                     EVARLATEMA"
BASE COUNT         1170 a         1508 c         1317 g          966 t
ORIGIN      
        1 ccaggcgggc cattacgcgg tccaccgggt cgggataagc ggcctcatcc tccttgctct
       61 cgacatagtt ggcgatgtga ccgcccagct cagcgccgta ggtcatcacc aaatcttcca
      121 ctgctccggg ttcaagcccc aaagacagcg cgcggctgag catggaaaca tccacgccgg
      181 cagccagagc ggaaccgaaa gcggccgggc tcataccgct ttgcatagcg gcataaagca
      241 cggtcaaatc ggcgccctgg tcagccagtt cggccgggtc cgggtagaga acgtccacgt
      301 cgtcaccgtg acgagcgaga acgcggtcca cctggtcgag ataacgcagg tcgtcgaggg
      361 cgtgataggc cgaaagggcc agatccgcat ccgaaacaac cgttgcacca ctgttggtct
      421 gatcgctcat accgttcata ctctttctcc tttgctagaa attgcattgg tgacctgagc
      481 caagacctgt tccctggtca tggcattggt cgggttgagg aagaccaaga caccaccctt
      541 ggtgccctgg tagtaactac gcagaggggt tgactcctcc gagtaggtgc ggagacgctc
      601 ccgaatcgtt tcgggggcat cgtccttacg ctggaaaagc gggcttgaac aagcgtcgca
      661 aataccggcg ttcttcggcg gctcgaattt caagtgatac gagcgaccgc aagattggtt
      721 ggtgcaagtg cggcgaagcg acaggcgctc caccaggtct gcctccgaaa gctcaagcca
      781 gacgatcttt tccagtgaca gtccccagga agcaagcagt tcgtccagga ggacggcctg
      841 tgctctggaa cgggggaaac catcgaggat ggcaccacgg gcattttccg gggccaaaag
      901 agcgcgcttg agaatcgtga gagccatctg gtcggggatc aaaagaccct tttccaccag
      961 agcttgcact tgcttgccga agtcagtctg agccgctatc tccttgcgga taagcgcgcc
     1021 agtgctgaca tgagataagc cgagagcggg tgccagctga ccagccaccg ttcccttgcc
     1081 tgcgccggga ccacccagga gggcgatcaa ccggggtctg gaggcaggga acagaggcca
     1141 gagcaggtac cgcttcatcc agaatttcgc ttgcgcgaag aaaccaccgg agctgagctc
     1201 cgacagatga ggcgatgttt tcatagacag ttacctttct ggtacgggag ccggcaggca
     1261 gccgacagac caggcacaga gcagacacac tgaagacgtg aagactgaag ctggaaccgg
     1321 cgagcaaagg cggcggtcca actcagggca gtctgcctct aggttgccag tgctgggaaa
     1381 tgaagaaatc tgcgggttga gaaaagcttg tgagaaaaat tgggaatgaa tagtgcattc
     1441 caccctactg atagacgctt caagaaggca agactaagtt ctcgagaaag ctcatgacag
     1501 ataggtctta gacaaatctc aggataatcg aagaaatagc tagcgcctgc tagcgctggc
     1561 cgcgcaaatc tacctgaaac cagttttatc acttggcagc ctgcattttg accatcggcg
     1621 ccccttttcg aaaaaggggc aaaccctaaa tccggctaca gatctagatc tcaagcagaa
     1681 gttaatatac cgtcgaggtg ttcacatcat tcccactcca tctcaaaacc ggcaaccaaa
     1741 ccatccccaa aagtgattta ctagcctttc atcaggtttc cggcaagaca tggtcttggg
     1801 gttctctaac cgctaaatct aaggagtcca aaatgacaac cacaactgtt atgggagctc
     1861 ttcctgcccg cgccgacgtc cccgtcgagc tgacctggga tctctccaag attttcgcca
     1921 ccgaggccga ctggaacgcg actttcgctt ccaccgaagc gctcgtggcc aagctgcccg
     1981 aattcaaggg caagctcaag cgttccggca agcagctcct ggccttcctc accgaacgtg
     2041 acaaggctgc cgagctcctg gacaagctct atgtctacgc cagcatgaag gctgacgaag
     2101 acactgccaa caccgccaac caggaacgcg aagagaagat tcgcgggctc tataccaagt
     2161 tcggcgcagc cgtctcctgg tccacgccgg aactcctgtc catgaagagc gagcgcctcg
     2221 cttccttcat cgccaagacg ccggcgctca acctctggaa gcagcagttc gacgagctga
     2281 accgcgagcg cgcccacgtg cgcagtccgg aagtggaaga gctcttggcc cagcaggcca
     2341 tggtcgccca gggtccgcag accatcttcg gcatgttcga caacgccgac atccagttcc
     2401 ccaaggtcaa ggacgccgaa ggcaacgagc accagctcac ccacggctcc tacggcgtct
     2461 tcctgcacag ccgcgacagc gtgctgcgtg agaacgccta ccggggcatg atggagacct
     2521 tcgagaagag ccgcaacacc gtcgctgcca tctacagcgc ccaggtgcag gccaacatct
     2581 tcaaggcgcg cgctcgtcgc tacggttctt cgctggaagc agcgctggga ccggacaacg
     2641 tgccgctctc cgtctacaag agcctgatcg agaccgccca cgcctacctg ccggtcttgc
     2701 accgcctggt ggcgctcaag gctcgtgaac tggccaagca gggcaccctg ccttccggca
     2761 agatccgcct ctgggacctg tacgcaccgg tggtcggctc cgtcgactac aaggtcgatt
     2821 acaccaccgc ccagggcatc attctggatg cggtgaagcc gctcggcacc gagtacgtgg
     2881 acaacctgcg caccggcttc gcctcgcgct gggtggacgt ggtcgagaac aagggcaagc
     2941 gctccggcgc atacagcggt ggcgccttcg gcactccgcc ctacatgctc atgaactgga
     3001 aggacaagct ggagcagatg ttcaccctcg cccacgaggc cggacactcc atgcactcct
     3061 tcttctcgcg ccgcggccag ccgttccagt acgccaacta caccatcttc gtggctgaag
     3121 tggcctccac ctgcaacgaa gcgctgctgg ctcaccacct gctttccacc gagagcgacc
     3181 cggcggtgaa gcgctacctc atcgatcacc agctcgatgc cttccgtgcc accttcttcc
     3241 gccagaccct cttcgccgag ttcgaaatgc tggcgcacga ggcggcggaa cggggcgaag
     3301 ctctgacggc cgacaagctg tccgagatct tcctcgacct gaacaagaag tactacggtg
     3361 actgcgttgc cgacgacgat ccggtcaaga tcgagtgggc gcgcatcccg cacttctaca
     3421 acagcttcta cgtctacaag tacgccaccg gcatctcggc ggcgacggct ctgtcggcgc
     3481 agatcctcaa ggaaggtcag ccggctgtcg atcgctatct gaagttcctg tcgggcggct
     3541 gctccgacta cagcatcaac ttgctcaagg gcgccggcgt ggatctctcc agcccggatg
     3601 ccatcaagtc ggcgctggat gtcttcacgg cctacctcga ccagctcgaa gagctgatca
     3661 aggctgacga agccaaggcc taacctcatc ctggggcggc gcagaaagag aactatctcc
     3721 ggtatgcgcc gcccctgtgg cgagccaaac aggcaatctc gggcacaggc tcagagcgga
     3781 ctaacacccc tttgagcctg tgcttttttc tttccaccca tgcctttttt gtttttcagc
     3841 cttactacat tctatggtcg cacaagcaat agtacgactg cgggcaacga tggcagacca
     3901 attaaggcag gcgggtccat gttttgcaag tctctcaagc aaaccggtca agcaaaccgg
     3961 tcaagcaaat ctctcccctc tcaagcaaat ctctcccctc tcaagcgaaa ctttcaagca
     4021 gccatttcaa gcacctacac aacttttgca gctcagccaa gacgatgagg aaaacccagc
     4081 ctgtgtctca cttgtggcgc ttcagtgtcg aagctgtgac tacaattaga acgctcaagc
     4141 tgatctttgt gagacagtga gcgccgtgta atcttttgca aaagccttca gtgacggtaa
     4201 ttacgcagtt ttccatggct gcaaaagtgc aaatgtaatt tccgcgtacc ccagaaagcg
     4261 cctgaatgca ggcaaatatg gagcatagag cgaaatcccg cataacaaat cggaagtaga
     4321 gaattaagta agagacaaat atgtgatcca gctaaggttc aattacctaa cttttgtctt
     4381 ttaacctgag caccagaaca aactttcata cagaactccc gcctacgaaa acttcctgcc
     4441 gacagcttgc tgtttgcagt gaaccgctca tgaggcaggg tttgttcatt ttagtttgaa
     4501 gaccaaggta aggcaggcag aaaccaccgc tacccgtgcg ctagctcgcg tacatggtgg
     4561 attgctcttt ttgaaactac cgagcgaaag ctcatttatc taaaatcaat ccctggagaa
     4621 tcttatgaat actcaacaag taaaacccag cgccacagtt cgccatgtac aaagacagct
     4681 cgaagtgggt ttcaactacg caatcattgg ctgtgcagtg acatacttcg accaccgcat
     4741 cacggaagaa gaaatagccg aaaccgtcct tcacttccag ggccgcaata tcacggccgt
     4801 gccggtctac gaagagggat gcctggactt cttcttcctc ttcccggccg atgcctctca
     4861 cctggagcat cctcgccgct tccccagtgc taacacccag gtgactcact tcatgatcgt
     4921 cacccccgat gaagtagcgc gtctggcaac ggaaatggcc g
//
LOCUS       DAOQNZ010000085         4951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7132_length_4951_cov_3.117851, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000085 DAOQNZ010000000
VERSION     DAOQNZ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7132_length_4951_cov_3.117851"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKW73_02375"
     CDS             complement(<1..229)
                     /locus_tag="PKW73_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72156.1"
                     /translation="MDAINNYYHQLTYLAPHLVLTVGILFAALWNLFFPKAKGMTPIV
                     SLIALGVSGFVLATQMTATEKLFGGVFTVDPL"
     gene            607..1779
                     /gene="carA"
                     /locus_tag="PKW73_02380"
     CDS             607..1779
                     /gene="carA"
                     /locus_tag="PKW73_02380"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNA72157.1"
                     /translation="MVDGAVTVKRFIRDEKGKLTWQTGKKACLVLEDGTIYEGEAFGA
                     SGTSLGELVFNTSLMGYQEILTDPSYAGQIITLTYPEVGNYGINEEDMESKKIYARGL
                     VIRHLSSVESNFRSQGSLDQFLKKHGIVGITEIDTRAVTRKIRDKGAMRCLISTELDA
                     GKDKDELLKRVKGLSEMTGQNLTGEVSTKASYKVGKGKFKVAVMDFGCKENILRELVK
                     RDIEATVYPSSVTAKEILESKPDGIFLSNGPGDPAACTDILKQLPALIESRIPIFGIC
                     LGHQLLSIALGATTFKLKFGHRGGNQPVKDLTTGKIEITSQNHGFAVDDKSMPDHLEL
                     THINLNDNSVEGFRHKTLPIFAVQYHPESSPGPHDSSYLFDRFVNLMAPLTLKSPH"
     gene            1794..2480
                     /locus_tag="PKW73_02385"
     CDS             1794..2480
                     /locus_tag="PKW73_02385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNA72158.1"
                     /translation="MEEGNKVYKLAIFDFDGTLVDSTPGIIDVMKVVVDEYQFEEGIL
                     EEWRHLVGVPLPKQMEIIFPDRDEDFHLEVANRYRAIYDTMAIEICPPFPHMKPLLRN
                     LKDAGVVVTIASSKRSNLVQVVIDHHDLNDYFAMVVGAQEVTHHKPHPESVHVTVAKL
                     DIPHEHTIVIGDSTFDLDMARNAGVDAIGVTTGVHTKEMLERSDPTHIVEDLVAVEKI
                     ILNGKNGKSV"
     gene            complement(2583..3425)
                     /locus_tag="PKW73_02390"
     CDS             complement(2583..3425)
                     /locus_tag="PKW73_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNA72159.1"
                     /translation="MKNVEFEPPGNEGIAEVRKRDDDPRVRLSLEAQASLKDNTSTAP
                     NKYNDWVPAITLDERGCKQYSHWAVEIREGQQYTIQKGDTLRDVARRSLGVTGNPDAT
                     SKEISAEMKRIAALNSDLAPLFNRRHGTLPPGVVLKLQEAKPVALAPAAETTASENGG
                     CPERERIVVRKGLIKAENCDRYELMENSAGIVRPGADVVVNGGARAFVFGGKVEARAG
                     SRIITVGGDIQAHPTARIKYVSEAGISESHAIFPAFPELDDKASKKAEQKPLNGPQAD
                     FSPA"
     gene            complement(3462..4547)
                     /locus_tag="PKW73_02395"
     CDS             complement(3462..4547)
                     /locus_tag="PKW73_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72160.1"
                     /translation="MTYDRPHETPTQPNPTRPDLNLVNLTSDNRNNNANDNRNNNAND
                     NRNYNGNANANDNRNYNGNDNRNNNANDNRNNNNNRADANSRSDANARSDANSRSNSD
                     SRSNSSATGGTSDARSNATGGNSDAKGGNASVSDNSVNKTTVYGGSAVAPNVYGGGFC
                     SEGGSAGVYVLGIGISGGKTTINESCLKKEDFQRTMTQVCRSADDKSQMAYQGWNLQL
                     QAGRELTNNPMANSVAMSAKRMADAKAKVALELDSVCGTMAATSDATMEAMKRSGLDK
                     PVIIVEEARNDAEQKMLDEALAKKVDELDKRVKTVENKPTAVIQQNNITFIEMDAPKP
                     VHKPPVKHKPPVEKKPADDCVDDKKKK"
BASE COUNT         1144 a         1277 c         1338 g         1192 t
ORIGIN      
        1 agagagggtc gacggtaaac acaccgccga agagcttctc ggtagccgtc atctgggtag
       61 ccagcacgaa gccggatacg cctaaggcga tgaggctgac tatgggtgtc atacctttgg
      121 ctttagggaa aaagaggttc cacaaagcgg cgaacaaaat gcctacggtc agcaccagat
      181 gaggcgccag ataggtcaac tggtgatagt aattgttaat tgcgtccatg ttgctaagaa
      241 gtcccctttg agcgcctcta attttacata ggtgaagcac ccgaaatcac aagttgaaca
      301 acaaagtaga cctaagcttt gctttaattt gaccgcagcc ctggtattga cagcctctga
      361 gctgattggc gcgggtactt tgcagccggg tgcaaggctt acaattgcct ggaaagcagg
      421 aaatattatg cctggatggc aagagaccga aaagaaggtg caagtttggg taagatagtt
      481 cttaccggcg gactctctta accagtcgat cgccaactga ttgagaggct agactcaaaa
      541 gcttgaaggc atatatcgca agcacacggg ttagctgacg gaagatttta gaaggaagca
      601 gttcaaatgg ttgacggcgc ggtgacggta aaaagattca ttagagacga aaagggcaaa
      661 cttacctggc aaaccggtaa aaaagcctgc ctggttctgg aagacgggac tatttatgaa
      721 ggtgaagcct tcggagcctc gggcaccagc ctgggcgagc tggttttcaa cacatccctg
      781 atgggctacc aggagattct caccgacccc agttatgccg gtcagatcat tacgctcacc
      841 taccctgaag tcggcaatta cggcatcaac gaagaggaca tggagagcaa gaagatatat
      901 gccagaggcc tggtcatccg ccacctctcc agcgtagaga gcaatttccg ctcccagggc
      961 agtctcgacc agtttctcaa gaaacatgga atcgtaggca ttaccgagat agacacccgg
     1021 gctgtgacac ggaagattag agataagggc gccatgcgct gtctcatttc caccgaatta
     1081 gacgccggca aagataaaga tgaactgctc aagcgcgtca aaggcttatc agagatgacc
     1141 ggtcagaatc tcaccgggga ggtctccaca aaggccagct acaaggtggg gaaaggcaag
     1201 ttcaaggtag ccgtcatgga cttcggctgt aaggaaaata tcctccggga actcgtcaaa
     1261 cgagacatcg aagccaccgt ctatccctct tccgtcaccg ccaaggaaat tctggagagc
     1321 aaaccggacg gtatattcct cagcaacggt cccggcgacc cggcggcctg caccgacatt
     1381 ctcaaacagt tgcctgctct aatcgagagc cgcatcccaa ttttcggcat ctgtctgggg
     1441 catcagcttc tctccatagc ccttggagcc accaccttca agctcaaatt cgggcacagg
     1501 ggcggcaacc agcctgttaa agacctgacc acaggcaaaa ttgaaatcac cagtcaaaat
     1561 catggttttg cagtggacga taagtctatg ccggaccacc tggaactaac ccatatcaat
     1621 ctcaacgaca actcagtaga gggcttcaga cacaaaaccc tgcccatctt tgccgtgcag
     1681 taccacccgg aatcgagtcc aggaccgcac gactcaagct atctctttga tcgctttgta
     1741 aatctgatgg cgccgctcac cctcaaatca ccacactaac cgctgaaaag caattggaag
     1801 aaggtaacaa ggtgtataag ctagccattt tcgacttcga cggaaccctc gtcgactcca
     1861 cccccggtat catcgatgtt atgaaggtag tggttgacga ataccagttc gaagaaggta
     1921 tccttgaaga atggcgccac ctggtgggag tgccgcttcc aaagcaaatg gaaattatct
     1981 tccccgaccg agatgaagac tttcatctgg aggtagccaa tcgctaccgg gccatctacg
     2041 acaccatggc cattgagatc tgcccgccct tccctcatat gaagccgctt ttgcgcaatt
     2101 tgaaggatgc cggcgtggtg gtcaccatcg cctcctcgaa acgttcaaac ctggtgcagg
     2161 tggttatcga ccatcacgac ctgaacgatt atttcgccat ggtggtggga gcccaggaag
     2221 tcacgcatca taagccccat ccagaatcgg tgcatgtcac cgtggccaaa ctggacattc
     2281 cccacgagca caccattgtc atcggggaca gcacctttga ccttgatatg gccagaaatg
     2341 ccggagtaga cgccatcggc gttaccacag gcgtgcacac caaagaaatg ctggagcgct
     2401 cggaccctac ccatattgtg gaagatctgg tagcggtgga aaaaattatc ctgaacggca
     2461 aaaacggcaa gtcagtctag ccaggctgat ttgccgttta gttcagtttt gccgcttgcc
     2521 taccggcggc aggcaagcaa cgattccgtc atgcaactat tccgtcatgc aagcactgtg
     2581 gctcaagccg gcgagaagtc tgcctggggt ccattgagag gtttctgttc ggctttcttg
     2641 gaagccttat catccaattc cgggaaggcc gggaagatgg catggctttc actgatgccg
     2701 gcttcgctca catatttgat gcgagctgta ggatgggcct gaatgtcacc gccgacggtg
     2761 atgatgcgcg aaccggcgcg ggcttccacc ttgccgccaa agacaaaggc tctggcaccg
     2821 ccgtttacaa ccacatcggc tccgggtctg acgattccgg ctgagttttc catgagttca
     2881 tagcgatcac agttctcagc tttgatcaga cctttgcgca ctacaatccg ttctctttca
     2941 ggacagccgc cgttttcaga ggcagttgtt tcggcggccg gggcgagagc caccggctta
     3001 gcctcttgca acttgagaac gacgcctggc ggcagggtgc cgtggcgacg attgaagagc
     3061 ggcgccagat cggagttgag agcggcgatg cgcttcatct cggcggagat ctctttactg
     3121 gtggcatcgg gattgccggt gacaccaaga gaacgtctgg ctacatctct caatgtatcg
     3181 cctttctgta ttgtgtactg ctgcccttcg cggatttcca cagcccagtg gctgtactgc
     3241 ttgcagcctc tttcatcgag agtgatggcc ggcacccaat cgttgtactt atttggcgcg
     3301 gtgctagtgt tatccttcag ggaagcctgg gcttccagag aaagtcgcac tctgggatca
     3361 tcatctcttt tgcgcacctc ggcaatgccc tcattgccgg gtggctcaaa ttcaacattc
     3421 ttcacggtgg gtttcctcaa ccaaagtaaa tgaagtgcga cctatttttt cttcttatcg
     3481 tctacacagt catcagccgg tttcttttcg actggaggct tgtgtttgac cggcggtttg
     3541 tgaaccggct tgggtgcatc catttcaatg aaggtgatgt tgttttgttg aatcactgca
     3601 gtaggcttgt tttcaacggt cttgacacgc ttatccagtt catcgacttt cttagccagg
     3661 gcttcgtcga gcattttctg ctcggcatcg ttgcgggctt cttctacgat gatcaccggc
     3721 ttgtcgagac cggagcgctt catggcttcc atggtggcgt cggaggtagc ggccatggtg
     3781 ccacaaacag aatccagttc cagagccact ttagctttgg catcggccat gcgcttggcg
     3841 ctcatggcta cactgttggc catgggattg ttggtcagtt cacgaccggc ttgcaattgt
     3901 agattccagc cctggtaggc catctgcgac ttatcatcgg ctgagcggca gacctgggtc
     3961 atggtgcgct ggaagtcttc tttcttgagg cagctctcgt taatggtggt cttgccgccg
     4021 gagataccga tgcccagaac atacacaccg gcgctaccgc cctcggagca gaagccgccg
     4081 ccgtatacgt tgggagctac ggcactgcca ccgtagacag tggtcttatt gacgctgttg
     4141 tcgctgacgg aagcattgcc tcccttggca tccgagttgc cgcccgtggc attgctgcgg
     4201 gcatcactgg taccaccggt ggcgctgctg ttactgcgcg aatcgctgtt gcttcttgaa
     4261 ttagcatcgc ttctggcgtt ggcatcgctt ctagaattgg cgtcggcgcg gttgttgtta
     4321 ttgtttctgt tgtcgttggc gttattgtta cggttgtcgt tgccgttgta attgcgattg
     4381 tcattggcat tggcattgcc gttgtaattg cgattatcgt tggcgttatt gttgcggttg
     4441 tcgttggcgt tattgttgcg gttatccgaa gttagattta ccagattcaa gtcgggtctg
     4501 gtggggttcg gctgggtagg tgtctcgtgg ggtctgtcgt aagtcattgg aaggattcct
     4561 catcgaaagc ggcagaaaat cgtcgcgggg ggaacgcgac cttgccttgg gggaaaattg
     4621 ggaactttgt actcgtgggg ggagtgcatt aagattgcca taggtttgtg acatatttgt
     4681 gagcggcacc agctctaaca acaagcccta tgcggttttt acgatgtaag gcgaaaacct
     4741 cgccgtcagg gcggcaccaa tctattgata agaagcggga actttgccgg gatgcctcta
     4801 gtcttcacca gagagaggct tgagaaattt agtgctggaa ttttcttgtg ttaactacct
     4861 tagttacaaa aaaggtttac tcgccctttc tccccgagag cgaagcggcg ccccagtacc
     4921 atgttccagc aaaacaagac agtacccggg g
//
LOCUS       DAOQNZ010000086         4938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7167_length_4938_cov_3.354905, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000086 DAOQNZ010000000
VERSION     DAOQNZ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7167_length_4938_cov_3.354905"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PKW73_02400"
     CDS             complement(<1..663)
                     /locus_tag="PKW73_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNA72161.1"
                     /translation="MKTLVADSKEKLGKGKIVNDFSIQVATVNGSGSQSSNNVLMRSI
                     FQMGIPVSGKNLFPSNIAGLPTWFTIRVNKDGYIARKAEIDILVAMNPQTAIDDVKAL
                     TPGSVCVSPVELKLDAIRSDVKHYQVPFSELAAKASDNLKLRKLLTNMIYVGVVAELL
                     NISHPEIENAITKQFEGKAKAIDLNLNAVKLGREWAKENLKKEDAYTVEPMDKTQGKI
                     IID"
     gene            complement(960..2606)
                     /locus_tag="PKW73_02405"
     CDS             complement(960..2606)
                     /locus_tag="PKW73_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNA72162.1"
                     /translation="MTTATKLTSHLILEQLLLEGASAVFHGPRCNPSPLVQLLNTSQV
                     LKCARALSERAASFMAIGHSQSTARPPVLVLSPGQGALNALPAIYTAKQIQVPLIVLC
                     EQQSTQIMNDDPVLALDNLALVSKLTKWSAEARSPVEVTRLIRRAFTEALAPPKGPVM
                     LSLPLDILYQYAQGEVINPPHVSPLGAAADNFLKKTAKSLVTAKNPCIIAGNEVNQFR
                     ARKEVVVLAEVLGCPVYTEPMPTGVNFPNRHPQFGGVLHFGLSTIKEKLKEHDLVLAL
                     GMQTRLSAEADKMSLFNPRAAIIQVNVEPTLAGKSLPCIAAATADIAETMTRLRAEIQ
                     LVADARCLSVAKIRAQETIKQITEERMKLEEELVYPSHNDPISLFWLLRTLDGVRPTG
                     SIIVNDILSIHADPCSVMSLETSSSYVSSNACIDGYGLPAGLGVQLSLTAQELPVIVL
                     TSDESIMQCSEALWSVAHYQLPIKIVIVSAGGAALLIQPQTPAQIGFPFNSPTIDFQK
                     MGQAMGLKVFTATIMEEMEEAITRLFEEEGPCVLNVKVSG"
     gene            complement(2662..3558)
                     /locus_tag="PKW73_02410"
     CDS             complement(2662..3558)
                     /locus_tag="PKW73_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA72163.1"
                     /translation="MRALLLPATTKDEAVRIGSSCKTYNLTLRARDRSGKACHLNFVG
                     LGAIYTPEAYRGRGYARALIELLEEEATAQGKDGLLLFSDIGSDFYSSCGFQEMSAAT
                     FTLELHPKGSVDAGDYQVVSLSDYPSAALLRHYSRWLARERLGVERSAAYFDYKIMKE
                     SYLHRHSKLSWPELLVLTAPELNGYAILESGGATVRVLEIIAQEPEHFFKPILHFALS
                     RKARRIRGWESVLSALAPGFDLRRYLLSSVESNRKILTDFYFEERTWGCPMILPLKPG
                     LEAELSDWCNFSPCPILELDHL"
     gene            3848..>4938
                     /locus_tag="PKW73_02415"
     CDS             3848..>4938
                     /locus_tag="PKW73_02415"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNA72164.1"
                     /translation="MSSIAAYKGHIVSPISASGSGDYSFIDYPRGLMVVTDGKLTALG
                     DFDQLRERSDLGAVLRQVTIEDFGKRLIMPGLIDMHLHLPQVTQTAKSGQHLLAWLNR
                     YIFPAECKFSDIDYARRIARWFFAELAANGTTLSVVFTTIHKEACNVAFEEAARTHSR
                     VIMGKVMMDLNAPAALCEVTQESLTGSMELARKWHNAHDCLLQYAFTPRFGVTSTPEL
                     LSGTGELWKRFPGTYMHTHLAEAREEIQTVAGLFPEARSYMDMYDRFGLAGQRSVFAH
                     AIHLDDGDLDCIKHRGAALAHCPSSNFFLKSGVFAYNRVRQCGCLFGLGSDVAAGPHM
                     SLFEVMKDANFIQPDDWIEPRELIYRSTLG"
BASE COUNT         1041 a         1270 c         1374 g         1253 t
ORIGIN      
        1 gtcgatgatg attttgccct gggttttatc catgggctcg acagtatagg catcttcttt
       61 cttgagattt tctttggccc attctctgcc cagtttgacg gcgttcaagt tgagatcgat
      121 ggctttggcc ttaccttcaa actgtttggt gatggcattt tcaatttccg gatgggagat
      181 attgagaagt tcagccacca cgccgacata gatcatgttg gtgagaagtt tgcgcagttt
      241 cagattgtcg ctggctttgg ccgccaactc actgaaaggc acctgataat gtttgacgtc
      301 gctacgaatg gcatccagtt tcagttccac agggcttacg cagacgcttc ccggtgtaag
      361 ggccttgacg tcgtcgatgg cggtctgtgg attcatggcc acgaggatgt ctatttctgc
      421 cttacgagca atatagccgt ctttattcac cctgatggtg aaccaggtcg gtagaccggc
      481 aatattggag gggaagaggt ttttgccgct caccggaatg cccatctgga agatggaacg
      541 catgagcacg ttattagagg attggctccc ggagccgttt acggtggcta cctggatgga
      601 aaagtcgttg actatctttc ctttccccag tttttctttg ctgtctgcaa ctagcgtttt
      661 cactgtgcgt atcctatttt tctggtgttc tggtctgtac ccttgggtgc tttctttgac
      721 ctgtctgaca ggcgcagttt caggttaagt ccgggctggt atgcctcccc aagcctgctg
      781 ctccctgggc acagtactaa agtttagccg ttcttgccgc agaagtgccc gtaaatacca
      841 atttcgctaa gcttttcgag atactttgca agtcaaacag gtactgactg aagagagccg
      901 gtacttaact attagaggga aattaaaaat gaaggaagac cggaactgag ggagttgcct
      961 cagccggata ccttgacatt gagaacgcag gggccttcct cttcgaagag gcgcgtgatg
     1021 gcctcctcca tttcttccat gatggtggcg gtaaacacct tcagacccat ggcctgtccc
     1081 attttctgga aatcgatggt gggactgttg aacgggaatc ctatttgggc cggtgtttgc
     1141 ggctgaatga gcagggcggc gccgccggca gaaacgatga caatcttgat gggtagctga
     1201 taatgggcca cagaccagag agcctcgctg cattgcatga tggactcgtc gctggtgagc
     1261 actatcaccg gcagttcctg ggccgtgagc gagagctgca ccccgagacc ggccggcaag
     1321 ccgtagccat ctatgcaagc attggaagaa acataggaag aactcgtttc caggctcatt
     1381 accgagcagg ggtcggcatg gatggagagg atgtcgttga cgataattga gccggtgggg
     1441 cgcacgccat caagggtgcg cagcagccag aaaagggaaa tgggatcgtt gtgggaaggg
     1501 taaaccagct cctcttccag cttcattcgc tcttcagtaa tttgtttgat ggtttcctgg
     1561 gctctgattt ttgccaccga taggcatctg gcatcagcta caagctgaat ttcagcacgc
     1621 aggcgcgtca tggtttcagc aatatcggct gtagccgcag caatgcaggg taagctcttg
     1681 ccggccagag tgggctccac attgacctgg atgatagccg cacgggggtt gaagagggac
     1741 attttgtccg cttccgccga gagtctggtt tgcataccga gagccagcac caggtcgtgc
     1801 tccttcagtt tttctttgat ggtacttaga ccaaagtgaa gaacgcctcc gaactgcggg
     1861 tggcgattgg ggaagtttac gccggtgggc atgggctccg tgtacaccgg gcatcccagt
     1921 acttcagcca gcaccactac ttctttgcgg gctcgaaatt gattgacttc gttgccggca
     1981 ataatgcaag ggtttttggc ggtaaccaga gatttagctg ttttcttcaa gaagttgtcg
     2041 gcggctgccc cgagggggct gacatggggc ggattgatca cttctccctg ggcgtactgg
     2101 tagaggatgt ccaggggcaa agacagcatt accggccctt tgggcggtgc cagggcctcc
     2161 gtgaatgcgc ggcgtataag ccgggttact tccaccgggg agcgggcttc cgccgaccat
     2221 tttgtaagtt tcgacaccag ggctaagtta tccagggcca gcaccgggtc gtcgttcatg
     2281 atctgagtgc tctgctgttc gcaaagcaca atcagcggta cctgaatttg ttttgccgta
     2341 taaattgccg gcaaggcgtt gagggctccc tgcccgggcg agagaaccag caccggcggt
     2401 ctggctgtgc tctggctgtg tccgatggcc atgaaactgg cggctctctc ggaaagtgcg
     2461 cgggcacatt tcaagacctg ggaggtgttc aaaagctgca ccagaggcga aggattacag
     2521 cgtggaccgt ggaagacggc tgaggctcct tccagaagca gttgttccag aatcaggtgg
     2581 ctggttaatt ttgttgcggt tgtcattgaa tactcagtct ctcaacgtgt ctatcctagc
     2641 ttactttgct cacaagggtt tttaaaggtg atcgagctcc aaaatagggc agggagaaaa
     2701 attgcaccaa tcagataact cggcttccag acctggtttt agtggcaaga tcatggggca
     2761 accccaggtt ctttcctcga aataaaagtc ggtgagaatc ttgcgattac tttcgacact
     2821 gctcaagagg tagcgcctca gatcgaagcc tggggcaagc gctgaaagaa cgctctccca
     2881 gcctctgatg cgccttgctt tccggctcag ggcgaaatgc agaatgggct tgaagaagtg
     2941 ttccggctcc tgagcaatta tctccagtac gcgcaccgtg gcgcctcctg attccagtat
     3001 ggcatagccg ttcaactcgg gagcggttag caccagcaat tcgggccaac tgagttttga
     3061 gtggcggtgt aagtaactct ctttcattat tttatagtcg aagtaagccg cgctcctttc
     3121 cacgcccagt ctttctctgg caagccaccg gctgtagtgg cgcagcaggg cggcggaggg
     3181 gtaatcgctc agactgacaa cctggtaatc tccggcatcg acgctgcctt tcggatgcag
     3241 ttcgagggtg aatgtagcag cgctcatttc ctgaaagccg cagctactgt agaaatcact
     3301 gccaatgtcc gagaaaagca atagaccatc cttgccctgc gccgttgctt cctcctccag
     3361 caattctata agggctcggg catagcctct gccccggtat gcctccggag tgtagatggc
     3421 tccgagaccg acaaagttca ggtggcaagc cttgccggac cggtctctgg ctctcagtgt
     3481 gaggttgtaa gttttgcagg aggagccgat ccttactgcc tcgtcttttg tggttgccgg
     3541 cagcagtaaa gctctcattt ttagatggcg acgaccccag gcgctggcgg tctgtctttg
     3601 taagtagtca aggtacctgt agtaagtaag acccccagac cagatccggt gtgtctcctt
     3661 cagtatcttg agaacctccg tgaagctaag aaaaccaaga aaaccaggtc tgaatagctc
     3721 tccctcgcgc cctccagagg gagattcttg cttgcacatt gtctcaggtg gtaggcttgg
     3781 cattttgccg gtcgggttgt tcgccgctct tttgtttgtt caacatctta cagggtacag
     3841 gcttcttatg agctcaatcg ctgcctacaa aggtcatata gtcagtccaa tttccgcaag
     3901 cgggagcggc gactactcct ttatagatta tcctcgcggc ctgatggttg ttaccgatgg
     3961 aaagctgacc gctctgggtg attttgacca gttgagagag cgttccgacc tgggcgccgt
     4021 tctcaggcag gtgaccatcg aggattttgg aaagcggctc attatgcccg gtctcatcga
     4081 tatgcacttg caccttcccc aggtgaccca gactgccaag tcggggcagc atctactggc
     4141 ctggttgaat cgttatatat ttcccgccga gtgcaagttt tccgatatcg attatgctcg
     4201 ccgaatcgcc cgctggttct ttgccgaact ggctgccaac ggcaccactt tgtcggtggt
     4261 cttcaccacc attcataagg aagcctgcaa cgttgccttc gaggaagcgg cccgcaccca
     4321 cagccgcgtc atcatgggca aagtaatgat ggatctaaat gcccctgcag ccttatgcga
     4381 ggtaacccaa gagtctctta caggctccat ggaactggcc cggaaatggc acaacgccca
     4441 tgattgttta ctgcaatatg ccttcactcc ccgcttcggg gtgacctcta cacctgagct
     4501 tttgtccggc accggtgaac tctggaagcg cttccccggt acttatatgc atacgcatct
     4561 agccgaagcg cgagaagaaa ttcaaaccgt ggccggactt tttccggaag cgcgcagcta
     4621 catggatatg tatgaccgct ttggtctggc cggccagcgc agtgtctttg cccatgccat
     4681 ccacctggac gacggcgatc tagactgcat taagcataga ggcgcagccc ttgcccattg
     4741 ccccagctcc aacttctttc tcaagagcgg tgtttttgcc tataaccggg tcaggcagtg
     4801 tggttgcttg ttcggactgg gttctgatgt ggcggccgga cctcatatgt ccctcttcga
     4861 agtcatgaag gatgccaatt tcattcagcc ggacgactgg attgagcccc gtgaactgat
     4921 ttatcgttcc acactggg
//
LOCUS       DAOQNZ010000087         4937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7168_length_4937_cov_4.097091, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000087 DAOQNZ010000000
VERSION     DAOQNZ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7168_length_4937_cov_4.097091"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..1094)
                     /locus_tag="PKW73_02420"
     CDS             complement(324..1094)
                     /locus_tag="PKW73_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNA72165.1"
                     /translation="MNLILIYGLLAAAAVSLVSLIGALTLTSSKISQERITPIFLSLA
                     AGAMVGNALLHLIPESIEHIIEHQDLGVNLHVISGLVVGGFFTFFAIDLFLHSVGKHD
                     THLVKPIGYMVLIGDALENLMDGLIIGAAFMLDPAVGVATTMAVIVHEIPIEMGDFAV
                     LIHSGFSPRKAILMNLLSGLVSLVGVLLAYAFSTHVENFPLFMGPVAAGAFLYMVGSS
                     LVPAIRQHSNLSSGFMHLIIALTGTGIMALLLLIDPSH"
     gene            1860..2327
                     /locus_tag="PKW73_02425"
     CDS             1860..2327
                     /locus_tag="PKW73_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72166.1"
                     /translation="MESILGLAFSSLVFMLVYQFLAPWFSGRAVTFSTPDLGPKFLFG
                     LLMTLGTALFMSFAVFAFVFLLLPFFVLAAAAPGPMTFLLVLGGTGLAVLLSVQVSHW
                     VMTIIAGKLMPSTITVPSAGAAWKAAWAPTVVTLLPYLFMVFLPLVGFSILAA"
     gene            2398..3456
                     /locus_tag="PKW73_02430"
     CDS             2398..3456
                     /locus_tag="PKW73_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72167.1"
                     /translation="MTKKITISKQDIEDAARQGAMAPRDVESLWQQLLQQKGGAREVE
                     ANSGFDFAQLAWYGGGVLVMIAMAWFMERAGSVFGTGSVFMLSLAYAVGFAALGHKLR
                     FKDGQKTPGGLLFTLAVLMAPLAVVSGLEAIGIAARLSGSQVALFAEIATLGIALVAV
                     NRVKVSILSAPVFGSLWLLPMTIADLVARPYLGPIFGFASNAYLLISMGIGVAILALA
                     FATDSRFGREPGADYSWWGYFFGVAAFWIPLSMLDSGSELGKLAYFGINLIMMVSSVL
                     LARKVFLLAGAIGSIYYIGHVLWTYFSDSLAFPLALIGVGIAVIYLGVQYRRQQASIE
                     AYVLGLIPEGVRKHLPRA"
     gene            3517..4587
                     /gene="corA"
                     /locus_tag="PKW73_02435"
     CDS             3517..4587
                     /gene="corA"
                     /locus_tag="PKW73_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNA72168.1"
                     /translation="MATPPTETVISLISYGPGYFLEKQVASVSELPELIKAGKVSWVD
                     IDGPVGAAVVQELGQLFSLHTLALEDVVNGRQRAKFEEYGQHHFLIAPMVTLTERLKT
                     EQLSLFFGAGFVVTFQELPDGDCLAPVRERLRNEIGNLRNLGADHLAYMIVDAVVDGY
                     FPVLQSLGDKLEELEDDILLRYDAKAPGKIYALKRDVIKLRRSIWPLREAVHALLRDS
                     SAMVSGDTRLYLRDCYDHAIRLLDLVEMYREMCTDLMDLHLSSNSNRMNEVMKVLAII
                     TTLFIPPTFVAGIYGMNFNPDKSPWNMPELNWFWGYPCALSVMAAMMLGLFFWMRWKG
                     WLKGAGMSENEKSLRRKLKELR"
BASE COUNT         1008 a         1339 c         1454 g         1136 t
ORIGIN      
        1 tatgtgcttc gggtgtaagc tgaggcggcg tctattaatg actcagatga attacaaaaa
       61 tccccgaaag atattataga acgtagtaga aacctgaaaa aagcaaggtg ccgggccccg
      121 ggatttaaat tcaccatcgg tgaatcaggg tttcgggcac ttgctttgcc ttgttttgcg
      181 gtgccttggt cttccctgtc atggcttgct ctagtctgct ctgctttgct ttctgctgaa
      241 gcccggagga agggatgctt gcccagagga caatcatccc ttctccgctg cgtttgcaaa
      301 ccgccggttt gctcagggct ctctcaatga ctggggtcga tgaggagcaa gagggccatg
      361 atgccggtac cggtcagggc gatgatcaga tgcatgaagc ccgaggacag gttggagtgc
      421 tgccgaatgg cgggcaccag ggaggagccg accatgtaca ggaaagcacc ggcagccacg
      481 ggacccatga agagagggaa gttttccaca tgggtactga aggcataggc cagaagcacg
      541 cccaccaggg agacaaggcc cgagagcaag ttcatgagaa ttgccttgcg ggggctgaag
      601 cccgagtgga tgagcacagc gaagtctccc atctcgatgg ggatttcgtg cacgatgacg
      661 gccatggtgg tggctacgcc gacggccggg tcgagcataa aggcggcgcc gatgatcagt
      721 ccgtccatga ggttctccag ggcatcaccg atcaagacca tgtagccgat gggtttgacc
      781 agatgggtgt cgtgtttgcc tacggagtgg aggaacagat cgatggcgaa gaaggtaaag
      841 aagccgccca ccacgagacc ggagatgaca tgcaggttca cgcccagatc ctggtgttcg
      901 atgatgtgtt cgatggattc gggaatgaga tgcagtaagg cattgccgac catggcaccg
      961 gctgccaggc tgagaaagat aggggtgatg cgttcttgcg agatcttgct ggaggtcagc
     1021 gtcagtgctc cgatcaggga gacgaggctg acggcagcgg cggcaagcaa gccgtagata
     1081 agaatcagat tcatgatatt tgtcctcagt ttgttcgcct gcctgccatt gcgactgcgt
     1141 caaacaccgg atcaccaaga gacggtaacc tgtgttttta ttgcgggcga tggtgcggag
     1201 gagaggtttc tgtttggtta gacttgtcgg aaggggtgga gaattacccc ttgaacctag
     1261 tggtaccggg gtagctgaaa caagccttct cttccgaaag gtatggtggg tttgagtttt
     1321 gggagtttct ctcgggccgc ggtagagctt cgggaagggg cagccgggca gtgaattcga
     1381 accctccggg gaaagggccc gggctcagat tagctggaat acacttagcg caggttaact
     1441 ttggcgagca aagctgattc ttcactcgcg cagctataat aatgacaatc gttttcattt
     1501 tgtccaactg tcttaaccac tgctgctttt tgaccggctg taggtccctg ctaattacta
     1561 aaggtaatgg tagaacagtc ttcgcgcaaa ctgcggtagc ggcagctccg aagcagataa
     1621 gccagaatat agctgcgtct gcttaattag tactcagtat ggtttttgaa atcaagaaat
     1681 caataggcac taattgctaa agaataaagg tcagtttttt ttcaacccca cagatcctcg
     1741 agccccagat ttagataact aagcctatat cagaaacagc ccagtgccca ctgacgcacg
     1801 cggcgcagct tagcttgatc tggaattgag tttctcgtgc aaccaacaaa ggagctctca
     1861 tggagtctat cctcggtctg gccttttcca gcctcgtctt catgcttgtc tatcagttcc
     1921 tggcgccctg gttcagcggc agagcagtga ccttcagtac ccctgatctc ggacccaagt
     1981 tcctcttcgg tctgctcatg accttgggca ctgcgctgtt catgagcttc gccgtgttcg
     2041 ccttcgtctt cctgctcctg cccttcttcg ttctggcggc ggcagctccc gggccgatga
     2101 ccttcctgct ggtgctgggc ggtaccggtc tggctgtgct tctgagcgtg caggtgtccc
     2161 attgggtcat gaccatcatc gccggcaagc tcatgccttc caccatcacc gttcccagtg
     2221 ccggtgcggc ctggaaagca gcctgggcac ccacagtggt tactctcttg ccctacctgt
     2281 tcatggtctt cctgcctctg gttggtttct ccatcctggc ggcttgagaa ggtttcgaaa
     2341 atgcattaga gccaccgcaa atggttgcag tggctcgcaa ccccctagag gtcctaaatg
     2401 accaagaaaa tcacaatttc caagcaagac atagaggacg cggcgcgaca gggagctatg
     2461 gctcctcgcg acgtggagag cctctggcaa cagttactgc aacagaaagg cggagccagg
     2521 gaagtggaag ccaattccgg ctttgacttc gcgcaactgg cctggtacgg cggcggcgtc
     2581 ctggtgatga tcgccatggc ctggttcatg gagcgtgccg gctccgtctt cggtaccggc
     2641 agcgtcttca tgctttccct ggcctatgcc gtgggctttg ccgccctcgg ccacaagctg
     2701 cgcttcaagg acggtcagaa gactccgggc ggcctgctct tcaccctggc ggtgctcatg
     2761 gctcctctgg ccgtggtctc cggtctggag gcaatcggca ttgccgcaag gctctcgggc
     2821 tcacaggtgg cccttttcgc tgaaatcgcc accctcggca tcgcccttgt ggcggtcaat
     2881 cgggtcaagg tctctatcct gtcggcaccg gtgttcggct cgctctggct tctgcccatg
     2941 accatcgccg acctggtggc ccgcccctat ctgggaccca tcttcggctt cgcctccaac
     3001 gcctacctgc tcatctccat gggtatcggc gtggccattc tggccctggc gtttgccacc
     3061 gactcacgct tcggtcgtga gccgggtgcc gactattcgt ggtggggcta cttcttcggc
     3121 gtggctgcct tctggattcc gctttccatg ctggacagcg gttcggagtt gggcaagctg
     3181 gcctacttcg gcatcaacct gatcatgatg gtcagctccg tgctgcttgc ccgcaaggtc
     3241 ttcttgctgg ccggtgccat cggctccatc tattacatcg ggcatgtgct ctggacctac
     3301 ttctccgact cgctggcctt cccgctggcc ctcatcggtg tcggcatcgc cgtcatctat
     3361 ctgggcgtgc agtatcgccg tcagcaggct tccattgagg cctacgtgct cggcctgatt
     3421 cccgagggag tgcgcaagca tctgccgcgg gcctgatttg cactttgagg cgggagttca
     3481 aagctcccgc ctcttcaaaa aaacgtgagg tacaagatgg caacaccgcc gactgaaact
     3541 gtcatatctc taatttctta cggaccgggc tatttcctgg aaaagcaggt ggcaagcgtg
     3601 agcgagctgc cggaattgat caaggccggc aaagtttcct gggtcgatat cgacggacct
     3661 gtgggtgccg cggtggtgca ggaactgggc caactattca gcctgcacac gctcgctctg
     3721 gaagacgtgg tcaacggccg gcaaagggcc aagttcgaag aatacggcca gcaccacttc
     3781 ttgattgcgc ccatggtcac tctcactgag cgtctcaaga ccgagcagct cagtctcttc
     3841 ttcggtgccg gcttcgtagt tacctttcag gagctgcctg atggggattg cctggcaccg
     3901 gtgcgagagc gtctgcgcaa cgaaattggc aatctgcgca atctgggcgc cgaccatctg
     3961 gcctacatga ttgtcgatgc cgttgtggac ggctatttcc cggtcttgca aagcctgggc
     4021 gacaaactgg aggaactgga agacgacatt ctgctcaggt acgatgctaa ggcgcccggc
     4081 aagatctacg ccctcaagcg ggatgtgatc aagctgcgcc gcagtatctg gcccctgcgt
     4141 gaagccgtgc atgcgctctt gcgtgattcc tccgctatgg tgagcggcga tacgcgactt
     4201 tacttgcgcg attgctatga ccatgccatc cgcctcctcg acctggtgga gatgtaccgg
     4261 gaaatgtgca cggatctcat ggatctgcat ctttccagca acagcaaccg catgaatgaa
     4321 gtgatgaagg tgctggccat catcaccacg ctctttatcc cgccgacctt cgtggccggc
     4381 atctacggca tgaacttcaa tcccgacaag tcgccctgga atatgcccga actgaactgg
     4441 ttctggggtt atccttgcgc tctgtcggtg atggccgcaa tgatgttggg gctgttcttc
     4501 tggatgcgct ggaagggctg gctcaagggc gccggtatga gcgagaacga aaagtctctg
     4561 cgcagaaagc tcaaggagct gcggtgagaa accccaagta gtttgagaag tcggagaact
     4621 gaagcaagta gtcagttttc cgactttctc ttttggggct taagatacgg aaagatatag
     4681 taaataaaga acgaaagtag ttggaaggaa aatgtgaagg aaagaaatgg ggaggcagaa
     4741 aatccgcctc cccgatggtt tactgtcggc ccagaggtga caaacgcggc gccggaaagc
     4801 gatcgggcgc cagaatgcga gcgggcgccg aagtcggtgc cgacgacgcc cggccgtcgg
     4861 cgcaacatga gccggcgtcg ttggcaagct tcatgagctt ttgcatgtcc gggtctgtac
     4921 tgtccatggc cagcctg
//
LOCUS       DAOQNZ010000088         4936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7172_length_4936_cov_4.649252, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000088 DAOQNZ010000000
VERSION     DAOQNZ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7172_length_4936_cov_4.649252"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PKW73_02440"
     CDS             <1..1160
                     /locus_tag="PKW73_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72169.1"
                     /translation="EAGKSAAPWIMVSLVLLTMTLTVGLSISLLKEPNKPKPEKSLDS
                     TFQRDTTSLKTTEEAASSVSLMDPMVSQPVITDWNKPYRVPTKTEEAEILFEFPNQRS
                     IGSLNYATDECAFDNDAKLLQRAREEELTIPLAARGTVSVDRNLPLAFKLLSFADSRQ
                     VLSGFSPNDLTQLEMSNSNLSDKEKLAPLLRLTGLKVLSLKHCELTDAGLKELDRLKN
                     LKKLDIADNPCSTKAIAELKLLKSLRDLSFSTDENCERLLIAAKETTRLESLSINGPM
                     KVANIELIGEIPFLKTLNISSAMITSTHLKELGKLENVEKLILTNCVSDNAHLKDYSY
                     LTNMRSLRLVNPVGLHLVGCKFIKRQLPKCNITLEEKPPGDGPSRVLAVED"
     gene            complement(1179..2582)
                     /locus_tag="PKW73_02445"
     CDS             complement(1179..2582)
                     /locus_tag="PKW73_02445"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA72170.1"
                     /translation="MTGGNESEDGQKDGAAGGASAGVGGGSLQGGAVGFQAEELAPRR
                     EIFAWAIFDFANSSYTTVVTTAIFNQYFVGTICQGMSAADATVKLTTALGVSNLLVVL
                     SAPLIGALADASGRKKLLLFLATALCVAATALLSLMHRGDVLPAMLLFIVSSFAFGST
                     EYLIAAFLPELAPREKMGKISALGWTLGYVGGLCVLALCHVYVSWAQKQGQQAFQYVP
                     MILLGVSAVFAISAAPTFIWLRERSLGQAGAGRSLVAVIGQSFKRVGDTISGARRYRD
                     LFVFLISLFAFSCGSTTVVAMAAVYAGQVMGFSTSETIAMIMLVNVAAAGGAFGLGLL
                     QDRIGSVRTLMLALLLWVVAIAMAFVAHDKVVFWLAATIMGAAMGATGAAARALVGQL
                     SPSGQAAEFFGLWGLTVKLAAVVGPISYGQITALSGGNYRLALLSTLGFFLSGLVAIF
                     FVNEKRGRQLAENSTRH"
     gene            2733..3713
                     /locus_tag="PKW73_02450"
     CDS             2733..3713
                     /locus_tag="PKW73_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72171.1"
                     /translation="MKKPQLILTILALSLTINAARAENNKLDRLDETAQTSFDQGDFK
                     RAVTNWNKLLTELSKDTESLEKEEQLARKQRAQKVLRALGQCALEQKDYVSATDLLNQ
                     ARTASVELAQPDPSLDQAFTKLSENYREIDPTSLGQEAATALKEVGASKIGVAKTDGG
                     EHIQVMLADKVVKPISANGVSHVGFDKVVSFDFSETPDGTVKIDNISGLKAKVKVWVD
                     IIASKLTLDQDQKPMAQVTGAKMGMSQSVSTQLPDEIYQPLIALISRVKNVFTPGGMA
                     PAGSPLDNVATGQTQPVENGSNQVTPASTIVPVVNSGQPQNNEIDAAPAQ"
     gene            3710..4528
                     /locus_tag="PKW73_02455"
     CDS             3710..4528
                     /locus_tag="PKW73_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72172.1"
                     /translation="MTRSNNLLLKFLSLVLCATAACPSAPAQVSNTVPPEEEPGGGQA
                     LLDDKGNLFMEEVEPAKPKPKPAKIPIDLSRVGVSVDGKGRIIPLKDESGVAGVIPFQ
                     SRIETSFTALPQPVFYAPVIVPNYGYGYPSYSTGLMAMPGYYPGLQRPPLGYYQPYLN
                     QGYNPYPLNYGGQNPYSTQFYGGYNPSVVPNNASYFNPYYSPYANSYFNPYANPYFNP
                     YPYGNYYGSPYTNFAQPTVGLRLGNFNAQLFAAPRTYGQYQSRTSMWNAFNLSF"
     gene            complement(4512..>4936)
                     /locus_tag="PKW73_02460"
     CDS             complement(4512..>4936)
                     /locus_tag="PKW73_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72173.1"
                     /translation="VIACSIRPLVIGGSKQRMQDNMKSSPRISVHVGTHFQKETKEKR
                     NTADLFADYSEKILASGYRAYLRGDKMEGRRFEHFVDKSKQARQQSPRPHRQPHQGSP
                     ARLRRLEGRCVWALTLRENLSRFVLTMCRMDKNLRRRG"
BASE COUNT         1344 a         1466 c         1184 g          942 t
ORIGIN      
        1 cggaagctgg aaaatccgcc gctccctgga ttatggtttc cctggtgctc ttaaccatga
       61 ctctgacggt gggcctgtcc atcagcctgc taaaagagcc caataaaccc aagcctgaaa
      121 agtcactgga ctcaaccttt caaagagaca cgacctctct caaaactacg gaagaagcgg
      181 cctcttccgt ctctctcatg gacccgatgg tcagccagcc cgttataaca gactggaaca
      241 agccttacag agtgccgacc aaaaccgaag aagcggaaat actctttgaa ttcccgaatc
      301 agcgcagcat cggttctctc aactatgcca ccgacgaatg tgccttcgac aatgacgcca
      361 aactcttgca acgagccaga gaagaagaat tgactattcc ccttgccgcc agaggaactg
      421 tttcggtaga tcggaatctg ccattggcct tcaaactact gtcctttgcc gactccagac
      481 aggttctatc agggttttcc cccaacgacc tgacacaact ggaaatgagc aattcgaacc
      541 tatctgataa agaaaaatta gcgccgctcc taagattgac cggtttaaag gtgctttctc
      601 tcaagcactg cgaacttacc gatgccggct tgaaagaact ggaccgcctc aagaatctca
      661 agaagttgga cattgcggat aacccctgta gcacaaaagc catcgccgag ctgaagctac
      721 taaaatcatt gcgagacctc agtttcagca ccgatgaaaa ctgtgagcgg ctgctgattg
      781 ccgccaagga aactaccagg ctggaaagcc tcagcatcaa tggtcccatg aaggtagcaa
      841 acatcgagct tatcggtgag atacccttcc tcaagactct caacatatca tcagccatga
      901 tcacgtcaac ccatttgaaa gaactgggta aactggagaa cgtcgaaaag cttattttga
      961 ccaattgcgt gagcgacaat gcgcatttaa aagactattc ctatctaacc aacatgcgct
     1021 ctctcaggct ggtaaacccg gttggcttac acctggtcgg ctgtaaattc atcaaacgac
     1081 aactgcccaa gtgcaacatc actctcgaag agaaaccacc gggcgacggt ccgtccaggg
     1141 tgctggcagt ggaagactga ggcaaccact gcggttatct aatgcctggt gctattctcg
     1201 gccagttggc gcccgcgctt ctcgtttaca aagaaaatgg caaccaagcc ggacaggaaa
     1261 aagcctaggg tggaaagcaa ggccaagcga tagttaccgc cgctcaaggc ggtaatctga
     1321 ccatagctaa taggacccac cactgccgcc aattttaccg tcagtcccca caaaccgaaa
     1381 aactcggcgg cctgtccaga gggagaaagt tgaccgacca gggctctggc agcggctccc
     1441 gtcgccccca tggccgcgcc catgatggtg gcagccagcc agaaaacaac cttatcatgg
     1501 gcgacaaaag ccatggcgat ggccacaacc cagagcaaga gagccaacat caaagtacgc
     1561 accgagccga tgcgatcttg caacagcccc agaccgaagg caccgccggc ggcggcaaca
     1621 ttcacaagca tgatcatggc gatggtttca ctggtggaaa agcccattac ctgtccggca
     1681 tacactgccg ccatagccac aactgtggta gaaccacagc taaaggcaaa aagactgatc
     1741 aagaagacaa agagatcgcg gtagcgccgg gcaccactaa tagtatcacc gacccgcttg
     1801 aaagactgcc cgatcacggc gaccaggctg cgcccggccc cggcttgccc caggctgcgt
     1861 tcccgcaacc agataaaggt tggagcagcg gagatagcaa agacggcgct gacgccaagc
     1921 aagatcatgg gcacatactg aaaggcttgc tgcccctgtt tctgggccca actgacatag
     1981 acatggcaga gtgccagaac gcagagaccg cccacatagc caagggtcca accgagcgcg
     2041 gatatcttgc ccattttctc ccggggagcc agctccggca aaaaggcggc aatcagatac
     2101 tcggtggagc cgaaagcgaa actggaaaca atgaagagca acatagccgg caacacatca
     2161 ccgcggtgca taagcgaaag cagggccgtc gccgccacgc aaagagcggt agccagaaag
     2221 agaagcaact tcttgcgccc gctggcatca gccagggcac caatcaaagg ggcgctcaga
     2281 accaccagca agttggagac gccaagggcc gtagtcagct tgactgtggc atcagcagca
     2341 ctcatgccct ggcagatagt accgacaaag tactgattga aaatagcagt ggtgaccacc
     2401 gtggtatagc tggaattggc aaaatcaaaa atagcccagg cgaagatctc ccgccgggga
     2461 gccaactcct ccgcctgaaa cccgaccgcg cccccttgca aagagccgcc cccgactccg
     2521 gccgaagccc caccggcggc cccgtccttc tgcccgtctt cagactcatt cccgccggtc
     2581 actcagcacc ttttctcgaa acccggcatt taaaccgcta tagcttccca ttacagatgg
     2641 gggtagagta acattagcag aacaaatttt ctcccgcccg aataacaccg ctcggtaaac
     2701 tcagtagata agtctaaaga agtggaaaaa cgatgaagaa accccagcta atcctcacca
     2761 ttctagccct cagtttaacc ataaatgccg cccgggctga gaacaacaag cttgaccggc
     2821 tagatgaaac cgcccagaca agctttgatc agggcgactt taaacgagcc gtgacgaact
     2881 ggaacaagct tctgacagaa ctgagtaaag acaccgagag cctggaaaag gaagaacaac
     2941 tggcccgcaa acaaagagcg caaaaagtgc tgcgggccct gggacaatgc gcccttgagc
     3001 agaaagatta tgtctccgcc accgaccttc tcaaccaggc ccgcaccgca tcggtagaac
     3061 tggcccagcc agatcccagt ttagaccagg ctttcaccaa gctgagtgaa aactacagag
     3121 aaatcgaccc caccagcctg ggacaggaag cggccacggc cctcaaagaa gtcggcgcca
     3181 gcaaaatcgg cgtggccaag accgatggtg gtgagcatat ccaggtcatg ctggccgaca
     3241 aagtggtgaa accgataagc gccaatggcg tttcccatgt aggcttcgac aaagtagttt
     3301 ctttcgattt cagcgagacc cctgacggca cagtcaaaat cgacaacata agcggcttga
     3361 aagccaaggt aaaagtctgg gtggatatca ttgcctccaa gctcaccctc gatcaagacc
     3421 agaaacccat ggcccaggtc accggcgcca aaatgggtat gagccagtcg gttagcactc
     3481 agttgcccga tgaaatctat caaccgctaa tcgctttgat aagccgcgtc aaaaatgtct
     3541 tcacaccagg cggcatggca ccggccggtt cacccctcga taatgtagcg accggacaga
     3601 cacagccggt ggagaacggc agcaaccagg ttacaccggc atctactatc gtgccggtag
     3661 ttaatagcgg tcaaccacag aataacgaaa ttgacgcggc accggcacaa tgacccgctc
     3721 gaacaacctg cttctaaaat ttctcagcct ggtgctctgt gcaactgcag cttgcccctc
     3781 cgcaccggcc caggtatcaa atacggtgcc gccggaagaa gagcccggcg gcggacaggc
     3841 cctgctcgac gacaagggca acctcttcat ggaagaggtt gaaccggcaa agcccaaacc
     3901 caagccggct aaaattccca tcgatttgag ccgggtcggc gtttccgtgg acggcaaagg
     3961 gcgcatcatt ccgctcaagg atgaaagcgg cgtagccggc gtcataccct tccagagcag
     4021 aatcgagacc tccttcacgg ctctgcctca gccggttttc tacgccccgg tcatagttcc
     4081 caattacggc tacggttatc cctcatacag cacggggctc atggccatgc caggctatta
     4141 cccgggcttg cagagaccgc ccctgggcta ttaccagccc tatctgaacc agggctacaa
     4201 tccttacccg ctcaattacg gcggtcagaa tccctattca acgcagttct acgggggcta
     4261 caacccatct gtcgtgccca acaacgcctc ttacttcaat ccctattaca gtccctacgc
     4321 gaattcttat ttcaacccct acgctaatcc ctatttcaat ccgtacccct atggaaatta
     4381 ctacggaagc ccctatacga attttgccca gccgaccgtt ggtctgaggc tgggcaattt
     4441 caatgctcaa ctatttgctg caccgcgtac ctacggacag tatcaatcac gcaccagtat
     4501 gtggaatgcc tttaacctct ccttctgagg tttttatcca ttctgcacat tgtcaaaaca
     4561 aaacgcgaaa gattttctct caaggtcaga gcccaaacac aacgaccctc tagtcgtcgc
     4621 aaacgagccg gggaaccctg gtgaggctgc ctgtgcggtc tgggtgactg ctgtcgagcc
     4681 tgcttactct tatcgacaaa gtgttcaaag cggcgacctt ccatcttgtc gccccgcaaa
     4741 taagctctgt atccggaagc caaaatcttt tcggaataat ccgcaaagag gtcggccgtg
     4801 ttgcgcttct ccttcgtttc cttttgaaaa tgagtaccca cgtgaaccga aattctcggt
     4861 gagcttttca tattatcttg catccgttgc ttgctgccgc caataaccaa tggcctgata
     4921 ctacaggcga tgactt
//
LOCUS       DAOQNZ010000089         4932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7183_length_4932_cov_3.250974, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000089 DAOQNZ010000000
VERSION     DAOQNZ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7183_length_4932_cov_3.250974"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKW73_02465"
     CDS             complement(<1..220)
                     /locus_tag="PKW73_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72174.1"
                     /translation="MTLLPDELPISLDKLDDYLKAILKIKLREFVHLNPIFNCIPSID
                     VASYVDQSKKTTLPVKAVAPVLPPAMPVE"
     gene            complement(362..1231)
                     /locus_tag="PKW73_02470"
     CDS             complement(362..1231)
                     /locus_tag="PKW73_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA72175.1"
                     /translation="MGVDSSCELTACESAPEEAVAASGSMADGGFVVSSPLYKADGEQ
                     AIGLSVLGGDASAIVREHMTLVRQIARKYSQYAPDSFEDLVQVGSIGLLKAIKYYDPG
                     RARSASFRTLATCYIRGEIRHYLRDHCSIVQVPRRLTEMNSQLSQLEEKLTRALDRTP
                     TVQELAAASGFSVQDVLEAQQSWEVRVHYESLDGNSEEDEREDRRALSEVVPDRRYQE
                     LFAASEDRELISQALKRLGERTRQIVEFVFFYDLSQKETAYVLGLSEMGVSRAVHSAL
                     KKLREIMAQDGRP"
     gene            1537..3465
                     /locus_tag="PKW73_02475"
     CDS             1537..3465
                     /locus_tag="PKW73_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA72176.1"
                     /translation="MKKATKTFTRLRVLWSRVPAQKQLLYLGLACIAALVTFTAWVWV
                     TRAQASINESVKLFGQGLAEALARGGAEALSNSGDLESLRYYILTQRGKLKTIAYIVF
                     EDNAGNILLDPTSKKDLKEIYPVYKQYKENPENFTVPGIYPSPPGYRSVTNIVVRMHK
                     NGQDLGLCWVGLYNDLFTILGTQQETTNFLTTIFCLVGVLGALGVWANYTLINAPLRT
                     LSQGASQIATGHFGHQIKVQRAGKEIDQLVNAFNYMSSRLQLYDKHNVDNLMAERNKF
                     ISERNKLELVLMSIADGVVVCDRDNRVQIVNRAATQIFDKEAKDLLGKPLVVCTEGPD
                     QPQICHVVQQFTDTNIAPGSLEPIVQQLQLGELTVRLHTAPIFLNKEFLGSVMIMQDI
                     TKQAELEKMKNEFMANVSHELRTPITSIKSYVDTLCNHGEKLDPDIHQEFLQIIDNEA
                     DRLMHLVNDVLELSRIEEANREFEMLPMDIHRACEAAIRSLNLMARDRGIELVFEQAE
                     NIPLASINQESIERVVINLLNNAIKYTPEGGKITVKVAESEGKEVAVHVKDTGIGIPE
                     DALEHVFDRFYRVEKKVHTIKGTGLGLTIVKKIIEKHGGRITVASAIGEGSTFSFYLP
                     ALAPSDDEMAAEESASLV"
     gene            3941..4429
                     /gene="coaD"
                     /locus_tag="PKW73_02480"
     CDS             3941..4429
                     /gene="coaD"
                     /locus_tag="PKW73_02480"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNA72177.1"
                     /translation="MARAIYPGSFDPMTLGHLDIVRRAHALFDEVIMAVVGNPGKSPL
                     LPMDIRKNLIEKAVKDLKGVKVEEFQGLTVDYARQTKTTVLIRGLRAISDFEAELGMA
                     QANKQLFPELETIFLMTKAEYSFISSSTVKEIARLGGDVSQFVPHPVNDYLKEHFSRG
                     TK"
     gene            4433..>4932
                     /locus_tag="PKW73_02485"
     CDS             4433..>4932
                     /locus_tag="PKW73_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72178.1"
                     /translation="MTMITAPTVQATNTFKQTTIYKHLDLLEHLIDDAAGIYKFKFIN
                     ADDFLDVLDKARARLPEELREAADVLAQRDEIIAESQRRSEQIITTARRQAETMLHES
                     ELLKAVQAEVERIRKQVVQEVEQMRREAIAEAERIRLEADEDAAKIREGADHYAEQVL
                     TRMENDL"
BASE COUNT         1322 a         1352 c         1220 g         1038 t
ORIGIN      
        1 tttccaccgg catagcgggc ggcaaaaccg gcgccacagc cttcaccggc agcgtggtct
       61 tcttgctctg atctacatag ctggctacat ctatagaagg tatacagttg aagatcgggt
      121 tcaaatgcac gaactcacgg agctttatct taaggatagc cttgagataa tcgtcgagct
      181 tgtctagaga tatgggaagt tcatctggta agagggtcat gaggaaagac ctcaaatcga
      241 ttaaagctag ggcattagcc acaagtggag aatgttagca gagattaacc gattgtcaac
      301 agaccattcg ggtgaaatca agaatttaat caaaacgagg tctaatgagg caagaaaagg
      361 gtcagggtct accgtcctgg gccattatct cccgcagctt cttaagggca ctgtgcaccg
      421 ctcttgagac tcccatctcc gataagccga gcacataggc tgtttccttt tgtgacaggt
      481 cgtaaaagaa aacaaactct acaatctgcc tggttctttc gcccagacgc ttgagcgcct
      541 ggcttatcag ttcccggtcc tcggaagcag cgaagagttc ctggtagcgc cggtccggca
      601 caacttccga aagggcgcga cgatcttccc gttcgtcctc ttccgaatta ccgtccaggg
      661 attcataatg cacccgcact tcccaggact gctgtgcttc cagcacgtcc tgcacggaaa
      721 atccggaggc agccgccagt tcctgcaccg tcggggtgcg gtcgagagcg cgagtgagct
      781 tttcttccaa ctggctcaat tgcgaattca tctcggtcaa acgccgcggc acctgcacta
      841 tagagcaatg gtcacgcaga taatggcgga tctcgcccct gatataacag gtggccagag
      901 tacggaaact ggctgagcga gcgcgaccgg gatcataata tttgattgcc ttgagcaagc
      961 cgatggaacc cacttgcacc aggtcttcaa agctatccgg cgcatactgg ctgtacttgc
     1021 gggcaatctg ccgcaccagg gtcatgtgct cgcgcacaat cgccgatgcg tcgccgccca
     1081 gaacactaag gccgatagcc tgctcgccgt ctgccttgta caaaggcgac gaaaccacaa
     1141 aaccaccgtc cgccatagaa cccgatgccg ccaccgcttc ttcaggagcg ctctcacagg
     1201 ctgtcagttc gcaagaagaa tcgaccccca ctgttaagtc cgcaggtctg tcggcaaatg
     1261 agtccgaatt acatccaggt cccggcgaga tgcccgctcg agacgcttcc gctctaaaca
     1321 aaacttcact tctggtgtga ttttttctag ccaaggtcat tactcccacg atctataagg
     1381 accctgagcc ccacaccaag ccccacggtt atgttccctt gacaacggtt ttttaaaccg
     1441 gtcatactgg ttgccttaag gcggggggct tcatctattg ctaaaagaac gcataattcc
     1501 tgcataatct aaatcgacca accacagaga atttaagtga agaaggccac caagacattt
     1561 acaagactga gagtgctctg gagcagagta ccggcccaga agcagctcct ctacctgggt
     1621 ctggcctgta tcgccgccct ggtcaccttc acggcctggg tctgggtaac cagagcacag
     1681 gcttctatta atgaatcggt gaaactcttc ggacagggac tggcggaagc tctcgcccgt
     1741 ggcggcgcgg aagccctcag taattcaggc gatctggaaa gcctgcgcta ttacattttg
     1801 acccagcgag gcaaactgaa gaccattgcc tacattgttt ttgaagacaa tgccggcaac
     1861 attctgctcg accccaccag taaaaaagat ttgaaggaaa tctatccggt ctacaaacaa
     1921 tacaaggaaa accctgagaa cttcacggtt ccaggcatct acccgtcacc accgggatat
     1981 cgctcggtca ccaacatcgt ggtgcgcatg cataagaacg gtcaagatct gggactctgc
     2041 tgggtaggtc tctacaacga cctcttcacc attctcggca cccagcaaga aaccacaaac
     2101 tttctcacca ccatattctg cctggtgggg gtgctgggcg ccctgggagt gtgggccaac
     2161 tataccttga tcaatgcgcc cttgcgcaca ctgtcgcaag gggcaagcca gattgccacg
     2221 gggcatttcg ggcaccagat caaggtgcaa cgagccggca aagaaatcga ccagttggtc
     2281 aacgccttca actatatgag cagccggctg cagctttacg ataagcataa tgtggacaat
     2341 ttgatggctg agcgcaacaa gtttatttcc gaaagaaata aactggagct ggtgctcatg
     2401 tctatcgccg acggcgttgt ggtgtgcgat cgcgacaacc gggtgcaaat cgtaaaccga
     2461 gctgccaccc agattttcga caaagaagcc aaagaccttc tcggcaaacc tctggtggtc
     2521 tgcaccgaag gtcccgatca accgcaaatc tgccatgtgg tgcagcaatt caccgacacc
     2581 aatattgcgc ctggctccct cgagccaatc gtgcagcaat tgcaattggg cgaactgacg
     2641 gtgcgcctgc acactgcccc gatcttcctc aacaaagagt ttctcggatc ggtaatgatt
     2701 atgcaggaca taaccaaaca agccgaactg gagaagatga agaacgagtt catggccaat
     2761 gtttcccatg aactgcgtac tcccattact tccattaaga gctatgtgga caccctctgc
     2821 aaccatggcg aaaaactcga tcccgacatt caccaggaat ttctgcaaat tatcgataac
     2881 gaggccgaca ggctcatgca cctcgtcaac gatgtgctgg aactgagccg cattgaagaa
     2941 gccaaccggg aattcgagat gctgcccatg gatatccaca gagcctgtga agcggccatc
     3001 cgctctctca acctgatggc gcgggacaga ggcatcgagc tggtcttcga acaagccgaa
     3061 aatattcccc tggccagcat caatcaggaa tcaatagaac gggtagtgat aaacctgctc
     3121 aacaatgcca ttaagtacac gccggaaggg ggaaagatta ccgtcaaggt ggcagagagc
     3181 gaaggcaagg aagtagccgt gcacgtgaaa gacaccggca tcggcatccc ggaagatgcc
     3241 ctggagcacg tcttcgaccg cttctaccgg gtcgagaaga aagtgcacac catcaaagga
     3301 acgggtctgg gcttaacaat tgtcaagaaa attatcgaaa aacacggtgg ccgcatcaca
     3361 gttgcctcag ccataggcga aggctccacc tttagtttct acctgcccgc cctggcgcct
     3421 tccgatgacg aaatggcggc ggaagagtcc gcctccctgg tctaacgagg cggtttgcaa
     3481 accggtaact aacccgaaac ttaaaccgat aacggttaag gtatctatcc ttatttagat
     3541 cggtagaccg ggtttagcag cctcccaaac aagacttttg taaccgcccc caaaatgccc
     3601 cctttaatct gcgggaacag gcaagaatta agcctcagcg cccgataata ttccaggcag
     3661 gggcttgccc ggaagaccac aaatacatcc agggcatata ccccggaaat ccattgggac
     3721 agccctgcaa atcctctgaa cattagcaac ttatccatta acgcatttag ttttcaagct
     3781 gggtcgtgtt atcatctcct cgttgacaag gtctctttgg actggcgtcc acggggatat
     3841 ccatcaggca tacgcaaaat gcctaaaccg gtagcaaaag caaaggcgaa agctaaagct
     3901 cagcgaccgg ttcagtaagc aggaaggagg aaattgagga ttggccagag ccatctatcc
     3961 gggctctttc gatcccatga ccctgggcca cttagatatc gttcgtcgcg ctcacgcgct
     4021 ctttgacgaa gtgatcatgg cagtggtcgg caaccccggc aaatctccct tattgccgat
     4081 ggatataaga aagaacctga tcgagaaagc cgtaaaagac ttaaaaggag tgaaggtaga
     4141 agagtttcag ggactgaccg ttgattatgc gcggcaaacc aaaaccacag ttttaatccg
     4201 aggactgaga gctatttcgg acttcgaagc agaactggga atggctcaag cgaacaagca
     4261 gctctttcct gaactagaaa ctatttttct catgacaaaa gccgagtatt cttttataag
     4321 ctcctccact gtaaaagaaa tcgcccgatt gggcggtgac gtatcccaat tcgtaccgca
     4381 tccggtgaat gactatctca aagagcattt cagcagaggc accaagtaaa aaatgaccat
     4441 gattactgca ccaacagtac aggcaaccaa caccttcaaa cagaccacga tctacaagca
     4501 tcttgatctg cttgagcatc taattgatga tgctgccggt atctacaagt ttaaattcat
     4561 caacgccgac gatttcctcg acgtgcttga taaagcccgg gctcgtctcc ccgaagaatt
     4621 gcgtgaagct gccgatgtgt tggctcagcg cgatgaaatc attgccgagt cccagcgccg
     4681 ctccgagcaa atcatcacca cggctcgccg ccaggctgag accatgctgc acgaatcaga
     4741 gcttctgaaa gccgtgcaag ccgaagttga gcgtattcgc aagcaagtgg ttcaggaagt
     4801 cgagcaaatg cgccgcgagg ccatcgccga agcggagcgt atcagactgg aagctgatga
     4861 agacgccgcc aagattagag aaggcgccga ccattacgcc gagcaagttc tcacccgcat
     4921 ggagaacgac ct
//
LOCUS       DAOQNZ010000090         4919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7208_length_4919_cov_4.836965, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000090 DAOQNZ010000000
VERSION     DAOQNZ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7208_length_4919_cov_4.836965"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..830)
                     /locus_tag="PKW73_02490"
     CDS             complement(57..830)
                     /locus_tag="PKW73_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72179.1"
                     /translation="MTSTKKKGGSDLALAEITAANWPPSSLTKVNDPYFKWSKTVMFD
                     LHGVVFDWESAFQDFASAHYGYSFHNVKREFYDMARDPNTPISPDQFSELFVEFVKRA
                     QGGYGDLQPLAGVVEQLELIKQAGINVVICTWTPGASDARPDGSATYGTGIAQQVTEE
                     LILKHLGHVVSKSDIMFNSTHGKKRVMLEERIPLIVEDNAETAVGVAQSAMGAIVMPQ
                     PYNEGLQFPNVLRLDSSNQLAYAVISFFDKLDDENMLAA"
     gene            complement(951..1979)
                     /locus_tag="PKW73_02495"
     CDS             complement(951..1979)
                     /locus_tag="PKW73_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA72180.1"
                     /translation="MKTNLSVRPSRRTSTARSLDRMIKKLGAKKGIDVSEARAQLRDK
                     LKELNAKRALGYLAYHGIIDLNLPDGTTVAILPDWHIPAHDKQVSWMVKEWLRDNQPD
                     ILIIIGDAVDMFGLSRWPKAPGVIANTQHEYDEVRRMLDEVIRLSGALHVFYILGNHE
                     DRNRRTQIDPNANVSGLIDPNTKEPFLNFHNLMGYTKSDPITFIYGALGEGGMAGGLV
                     VNGDIDFIHGLKVRSKPGQSPAAESDNSGRSVWHGHTHRLGKRSRRTTKRGVIRSYEG
                     GHLSDRRHAYMGYAIKPNWTMGVSKGCVHGGKLHIQLLPILPVTDAGGQRRHAMVDGD
                     KVYKTAVY"
     gene            complement(2524..3546)
                     /locus_tag="PKW73_02500"
     CDS             complement(2524..3546)
                     /locus_tag="PKW73_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNA72181.1"
                     /translation="MNSEEERLRIKTQSTSQAPKDLYYILGVSPNASTGEICDAYTNL
                     SDKFGPHADTAEMDPDMLTRTWRDITHAYEVLSDNTKRREYDKNSAVFRQQTSDVRAL
                     WSKVTSGVQQVVQSTQQSQTQEIKNVQGKIQAQSLEMEIDVTLKEAIKGCHRQLTISD
                     PKACEECINMKPVNRMQCPTCRGVGYFTVERRLEIDLPKGLFDGMEIRRTAQGKWDMR
                     AGAHGDLILKIKLRQHPVLGVIDKDVTINVPVTLYEAMLGAEIEIPTATGKVVMKIQP
                     LTQPGRVYRLKGLGIAGGDQLVTIDVLIPQQLNGEEVKLYQKLKELSREKNPREALFL
                     AVSQSA"
     gene            complement(3883..>4919)
                     /locus_tag="PKW73_02505"
     CDS             complement(3883..>4919)
                     /locus_tag="PKW73_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72182.1"
                     /translation="ILEGSLATTNDVFQHPASRSTFPIDLISSLTQSALAMRERQLPA
                     PLKEVGTIEKSKSLAMMAPQAIKNKLLYRPAQTAALAYLPNIGAGADSKSTAKNLGSQ
                     LEGERACSYSGSLAEEQERRARLMREELAEHCELSTLANLLELVQVMGESGSPEIEHP
                     AQTSRPPTVENSLTKLAQLAGAGQANWAMEKLEKWQSSRSESQKLKDEPLPQPLSSTE
                     ELAEAGRVADLAMANDQKSANYRAILGLSTIKPSGLEQSLSAISMVPLIFTKVASLAQ
                     TGIEKGNGGGRIKSLADTLFYGQALEIRQTMLTRQAHMLISRRNYAILSGNRLLFDFT
                     ELLKRRLSGI"
BASE COUNT          924 a         1270 c         1474 g         1251 t
ORIGIN      
        1 accgtcacgc ccttgaggcg agtagctcga ttagccatga tacatactcc ttgttgttat
       61 gcggccagca tgttctcgtc gtcgagcttg tcgaagaacg agatcacggc gtaggccagc
      121 tggttagaag agtcgaggcg caggacgttg gggaactgca gaccttcgtt atacggttga
      181 ggcatgacga tcgcacccat ggcgctctga gcgacgccca cggcggtctc ggcgttgtct
      241 tcgacgatga gcgggatgcg ctcctcgagc atgacacgct tcttgccgtg agtgctgttg
      301 aacatgatgt cgctcttgga gacgacatgc ccgaggtgct tgaggatcag ttcctcggtg
      361 acctgctggg cgatgccggt gccgtaggtg gcggaaccat caggacgggc gtcggaggcg
      421 ccgggcgtcc aggtgcagat gacgacgttg atgccggcct gcttgatcag ctccagctgc
      481 tccaccacac ccgccagcgg ctgcaggtcg ccgtagccac cctgtgcgcg cttgacgaac
      541 tccacgaaga gctcgctgaa ctggtcgggg ctgatgggag tgttggggtc gcgagccatg
      601 tcgtagaact cgcgcttgac gttgtggaag ctgtagccgt agtgcgccga cgcgaagtcc
      661 tggaaagcgg actcccagtc gaacacgacg ccgtgcaggt cgaacatgac ggtcttggac
      721 cacttgaagt aggggtcgtt gaccttggtg agcgaactcg ggggccagtt ggcggcggtg
      781 atttccgcaa gcgccagatc cgacccgccc tttttctttg tgctagtcat aattgactcc
      841 ttagaagtta gacaggagac accacgccgc tcccagaaca aaatgccgct cctccggaag
      901 agggcggcat tctgcttgga gtggcttcgg tgcctcagtg aatggtcgga ttagtagacg
      961 gcggtcttgt agaccttgtc gccgtccacc atggcgtgac gccgctgacc accggcatcg
     1021 gtgaccggca ggatgggcag gagctggatg tggagcttgc cgccgtgcac gcagcctttg
     1081 ctcacaccca tggtccagtt gggcttgatg gcgtaaccca tgtaggcgtg acgccggtcg
     1141 gacaggtggc caccctcgta ggagcgaatc acgccgcgct tggtggtgcg acgggagcgc
     1201 ttgccgagac ggtgggtgtg tccgtgccag acgctgcgtc cgctgttgtc ggactcagca
     1261 gccgggctct gaccgggctt ggagcgcacc ttcaggccat ggatgaagtc gatgtcgccg
     1321 ttgacgacga gaccgccagc catgcctcct tcaccgagcg cgccgtagat gaaggtgatg
     1381 gggtcactct tggtgtagcc catcaggttg tggaagttga ggaacggttc cttcgtgttc
     1441 gggtcgatga gaccggacac gttggcgttg ggatcgatct gagtgcggcg gttccggtcc
     1501 tcgtggttgc cgaggatgta gaacacgtgc agggcgccgg agagacgaat cacctcgtcc
     1561 agcatacggc gaacttcgtc gtactcgtgc tgggtgttgg cgatgacgcc cggtgccttg
     1621 ggccagcgag acaggccgaa catgtcgacc gcatcgccga tgatgatcag gatgtccggc
     1681 tggttgtcgc gcagccattc cttgaccatc cagctgacct gcttgtcgtg ggcggggatg
     1741 tgccagtccg ggaggatggc caccgtggtg ccgtcgggca agttgaggtc gatgatgccg
     1801 tggtaggcga gatagccgag cgcgcgcttg gcgttgagtt ccttcagctt gtcgcgcagt
     1861 tgcgcgcgag cttcggaaac gtcgataccc ttcttagcgc cgagcttctt gatcatccgg
     1921 tccagggacc gagcagtgct cgtgcgccgc gagggtcgta cagaaagatt ggttttcatg
     1981 tgtgactcct ttggcctctt agcagcgagg cttcagttaa atggttaaag ccccgtctga
     2041 cgaggccgcg tttgacttac agaagtaagc gggcttatgc acgcagccct gtcggaggga
     2101 atgtgccaga ttccttccag gaaattttga atccgactac tccggtaccc atgctcaacc
     2161 gactctgaaa agagatgtga tggtcggagg gtagggtgaa tgccttgggg gtttgagggt
     2221 tttcctagag cattttggag gagtggataa ctcctagacc ttaaaggttc cccgagaacc
     2281 agtacaacta cggttttcca gtcttagaga gagataaagt gtgttaactt ggtggcgcag
     2341 ccgcacttag atcagcatga tttacgggct ctaatcttgg ttgggctgaa ccggcggcac
     2401 ccggcttgcc aagcggtggt gtgccatgat tatttttggg gtaccctgct agttgtgtta
     2461 agaacattgc gcggcttatg gcaggcgcat ttaagggttg gcgcggcttg cgcgagcccc
     2521 ttcctaagcg ctttgactga cagccaggaa taatgcctca cgagggttct tttcgcggga
     2581 aagctctttc aatttctggt aaagctttac ctcttccccg tttagttgtt ggggaatgag
     2641 aacgtcgatg gtgaccagtt gatcgccgcc ggcaatgcca agccctttca gcctgtagac
     2701 tcgaccgggc tgggtgaggg gctgtatttt cattaccact tttccggtag cagtgggaat
     2761 ttcaatttcg gcaccgagca tggcttcata gagcgtgacc ggtacattta tggtgacgtc
     2821 tttatcgatg acaccgagaa ccggatgctg cctcagtttg attttgagaa ttagatcgcc
     2881 gtgggcgccg gctcgcatat cccatttgcc ctgagctgtg cgtcttatct ccatgccgtc
     2941 gaaaagccct tttggcaagt cgatttccag gcggcgttcc acggtgaagt agcctactcc
     3001 tctgcatgta gggcactgca tgcggttcac cggcttcatg ttgatgcatt cttcgcaggc
     3061 tttcggatcg cttattgtga gctgcctgtg gcaacctttg atggcttctt tcagggttac
     3121 gtcaatctcc atttccagag attgggcttg aatcttgccc tgtacgtttt tgatttcctg
     3181 agtttgtgat tgttgggtgc tctgcaccac ctgttgcacg ccggaagtaa ccttgctcca
     3241 gagtgctctc acatcggagg tttgctgccg gaagacggcg ctgtttttgt cgtattcgcg
     3301 ccttttggta ttgtctgaga gcacttcata ggcatgggtg atgtctcgcc aggtgcgagt
     3361 cagcatgtcc gggtccattt cggcggtatc ggcatggggt ccgaatttat cggacagatt
     3421 ggtgtaggcg tcgcatattt cgccggtgga ggcattcggg cttacgccga gaatatagta
     3481 aaggtccttc ggggcctgag aagtgctctg ggtcttaatt ctaagtcgtt cttcttcaga
     3541 gttcatggct tcgacacaat cggctcattg tcctgggtag taagtgaatt tgcctacact
     3601 cccatgagta taagcgcacg gtcaaaaatt gtggactgcc taagcaggtt gctttgatgg
     3661 cttgtttaat tgtgtgctgg taatgaattg aaccgggtga tgaaccgggg gttgagaaaa
     3721 tacatgccgc catctgccat gtatatttgt tctatatttg cccagacaat gagtctttta
     3781 tcgcttatta tcagtctgcg cttcagattt tattattgaa gagccacccc tactcggaaa
     3841 aaccttacac aattgctttt ctgagagtgc tttctaactg cttcagatgc cgctcagcct
     3901 gcgttttaga agctctgtga aatcgaagag gaggcgattg ccgctgagaa tggcgtagtt
     3961 tctgcgactg ataagcatat gggcttgcct ggtcagcatt gtctggcgaa tttccagggc
     4021 ttgtccatag aacaaagtat cggccaggct ctttattcgc ccgccgccat tgcctttttc
     4081 aattccggtt tgtgctaaag aggccacctt cgtgaaaatg agcggcacca tggaaatggc
     4141 actgagactt tgttccaacc cggaaggttt gatagtgctc agaccaagaa tggctctgta
     4201 gttggccgac ttctgatcat tggccatggc taaatcggca acccttccgg cttctgccag
     4261 ttcttccgta ctgctcagag gctggggcaa cggctcgtct ttaagttttt ggctctcgct
     4321 gcggctgctc tgccactttt ccagtttttc catggcccag tttgcctgcc ctgctccggc
     4381 cagttgagcc agcttagtca ggctgttttc cacagtcggg ggccgggaag tttgagccgg
     4441 atgctcaatt tcggggcttc cgctctcgcc cattacctgc acgagctcaa gtagattagc
     4501 cagagtggaa agctcgcaat gctccgccaa ctcttcacgc atcagccggg ctcgcctttc
     4561 ttgctcttcc gccaggctac cgctataact gcaagcccgc tcgccttcga gttgcgaccc
     4621 gaggtttttg gcagtgcttt tgctgtctgc ccccgccccg atgttgggaa gataggccag
     4681 ggcagccgtt tgtgccgggc gatagagcaa cttgttttta atggcttgcg gagccatcat
     4741 agccagactt ttgctcttct ctatggtacc gacttccttc aacggtgccg gtaactgccg
     4801 ctctctcatg gccagggcag actgtgtcag tgatgaaatc agatcaatgg ggaacgtact
     4861 gcggctagca gggtgctgaa aaacatcgtt tgtagtggca aggctccctt ccaggattt
//
LOCUS       DAOQNZ010000091         4912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7228_length_4912_cov_3.437925, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000091 DAOQNZ010000000
VERSION     DAOQNZ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7228_length_4912_cov_3.437925"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PKW73_02510"
     CDS             <1..1115
                     /locus_tag="PKW73_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72183.1"
                     /translation="IGEYSTVIKLNPQSPKPYYNRAMAYKKLGKLELAGKDLQQFDKL
                     SGKLVAREQESAVLEAVHKQVREGNRATAIEALESANKSFPMAGLAYNLGLLYRDSNN
                     YPRALKAFAQAIELGIKDQEKTSSAAFAYLDRGMLLAKLGKYKDALRDFTSLIALADK
                     GHPVNSVNSLLKEYRKLALGERARTYVMLKEPDKALADCNQLIALNPKAGLAYQIRSA
                     IYKVQKKDALAKADEAKAKKFGATAAIDTSLLLSSPEAAYRKAYEQLTQIIKEHPAEM
                     HPYNERAFLSLNAKYYKAAIEDFNVILKKTPQDVKALLGRGQCYFFLSDYASAGDDFD
                     KALAVDPQKSYAAHTWKARIKEKLSPREDHSGVRAD"
     gene            complement(1245..1574)
                     /locus_tag="PKW73_02515"
     CDS             complement(1245..1574)
                     /locus_tag="PKW73_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72184.1"
                     /translation="MSDDFDKAWVSNYVPKGTVQDSINQIKEYAYRSFDALDANGNGF
                     IERGELEVVLKADGTPEREKSFITFLLTNQEAISAQVREDPLGPKDGISRLDLDAYFK
                     LLLASMG"
     gene            complement(1658..2866)
                     /locus_tag="PKW73_02520"
     CDS             complement(1658..2866)
                     /locus_tag="PKW73_02520"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNA72185.1"
                     /translation="MNRRAGNALFLVLLLSLAMLRADAQQKQYEWVPGSRQARWVNPA
                     GNQTGSQYQSQYQRQYYQGRQPGYPAYPQSQGSGSMRQTAVQAQPQPQSVQSRSAQPA
                     AQTASAQDFSDTVNVGGVERSYLIHVPRGYDSKRSYPLVMAFHGLGMNGQLMYGMSGL
                     TGVADRKGFVVVFPNAQGGRWQDGLTAGNDDVSYVEAMLAKLSRTVNIDSRRVYACGI
                     SNGGYFTQLLSLSIPEKIAACAVVASTLTRGGANCAGGRSVPIVFFLGTEDPLIPWDD
                     GRKRDLGKLGEVLGIGALGSLDGPLAKMGGLNSAPETIDFWINHNGASGNPRVSQEPD
                     SSPRDGTRVEKQVFGSGASSVVLYKIVGGGHTWPGCLNLRAISDISGPICQDIDASET
                     MWEFFRNFSR"
     gene            3125..3781
                     /locus_tag="PKW73_02525"
     CDS             3125..3781
                     /locus_tag="PKW73_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72186.1"
                     /translation="MKAPRCKFPTGSRSTQSKLALAVLAASACLLSLTPVSPLPAQAQ
                     MPPGPEAVTAPPLVETAEGPLREKRQTLVQKIQSAKDQGIGIASYMAAFNFIEEMVKS
                     KAAEADIGKRIDSLDNSLEDQFKRSAILKTQRPTPPIAESSPPPSAMMGGGGLPDLGG
                     GGGDIISKLKSKYGGQIPPNLKDKLPPGLQEKLSGLGGDPSQLLNDPNLKDILKGLKK
                     "
     gene            complement(3800..>4912)
                     /locus_tag="PKW73_02530"
     CDS             complement(3800..>4912)
                     /locus_tag="PKW73_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72187.1"
                     /translation="RRADGLAERAYRTAIELRADHCESLFNLLAFLKKGGVSFVKDAR
                     LAVKRFPKKFESHYYLVVALLRENKIEQAYNAATEAVTVFPSHAALQECRGWLLAKLG
                     RYEDSVRAYSQQIALDPDCGEGYLNRAQVSLKLGDDKGAAQDFQRALGLERLYVDKSM
                     DVVFLTREELLLYDYRGDGDNDAIEARIVPFCLARGFYWRRWNHGNAIGHFQMAIKAA
                     PNDYRGYLYRAEFFADLEQDLDALTDFDSACRLAPNNFWCFTRRAYSRAVLGLNAGAL
                     ADFTKGMRGGRDPYSLCARGMVYASMGDYRLAIADFDQVLSYGDKRVERQALKNRCRA
                     KFKAGDKFGAISDFLELALRYGMSNDISECLRNTLK"
BASE COUNT         1266 a         1444 c         1200 g         1002 t
ORIGIN      
        1 ccattggcga atattccacc gtcatcaagc tcaatccgca gtctcccaag ccttactaca
       61 accgggccat ggcttataag aagctgggca agctggaact ggccggcaaa gatttgcagc
      121 agttcgacaa actctccggc aaactggtgg ctcgcgagca agaaagcgcc gtgctggaag
      181 cggtgcacaa acaggtgcga gaaggcaatc gcgccaccgc catcgaagcc cttgaatcag
      241 ccaataaatc cttcccaatg gctggtttgg cttacaatct cggtctcctc taccgcgatt
      301 cgaacaacta tccccgcgcc ctgaaagcct ttgcacaggc tatcgagctg ggcatcaaag
      361 atcaagaaaa aacatccagt gccgccttcg cctacctgga ccgtggcatg ttgctggcca
      421 aactgggcaa gtataaagac gccttaaggg acttcaccag cctgattgcc ctggccgaca
      481 aaggacaccc ggtcaattcg gtaaactcac tgctcaagga ataccgcaaa ttagccctgg
      541 gtgagcgcgc ccgcacttat gtcatgctca aggagccgga caaagccctg gccgactgca
      601 accaactcat cgccctcaat cccaaagccg gactggctta ccagattcgc agcgccatct
      661 acaaagtgca gaagaaagac gccctggcca aagccgacga agccaaggca aaaaagttcg
      721 gggccacagc cgccatagac acatctttgc tattgagttc accggaagcc gcctaccgca
      781 aagcttacga acagctcacc cagatcatca aagaacaccc cgctgaaatg cacccctaca
      841 acgagcgcgc ctttctttct ttgaacgcca agtactacaa ggcagccatt gaagacttca
      901 acgtcatcct caagaagacc ccccaggatg tcaaagccct tctcggtcgg gggcagtgct
      961 acttctttct aagcgactac gcaagtgccg gcgatgactt cgacaaggct ctggctgtgg
     1021 acccgcaaaa gtcatatgcc gcccatacct ggaaagcccg catcaaagag aaactttcac
     1081 ccagagaaga ccacagcggc gtgcgggctg actagttaag taccggaata agtaccggaa
     1141 aaggtctggg tctgaactga ataagaaccc aaaaggggca cgagtcttag acccgcaccc
     1201 cttccggcaa gagatacacg aaccacctgg gaaactttac agcttcaacc catactggct
     1261 agcaaaagct tgaaataggc atccagatca agcctggaaa taccgtcttt gggtccgagg
     1321 ggatcctcgc gcacttgagc ggaaatcgct tcctgattgg tgagcaggaa ggtaataaac
     1381 gacttctcac gctccggtgt accgtcggct ttcagcacaa cttccagctc gccgcgctca
     1441 atgaaaccat tgccgttggc atcaagggca tcaaaagacc gataggcata ctcttttatc
     1501 tgattgatgc tgtcttgcac cgttcctttc ggcacataat tggaaaccca ggctttatca
     1561 aaatcgtcag acacggcata ctcccatcaa attttccaaa agagccgcac tcggccccct
     1621 aagctaataa tctaccaggc aaaagacgtg ggtaaactca gcgggaaaag tttcggaaaa
     1681 actcccacat agtttcagaa gcgtctatat cctgacaaat cggcccgctg atgtcactga
     1741 tggcacgcaa attcaaacag cctggccagg tatgaccgcc ccccacaatc ttgtagagca
     1801 ccaccgagct tgctccgctg ccgaagacct gcttctccac tctagtcccg tcccggggcg
     1861 agctgtccgg ctcctgagat acccgaggat tgccgctggc accgttatga ttgatccaga
     1921 aatcaatggt ctccggtgca ctattcaagc cgcccatttt ggcgagtggt ccatccagag
     1981 aaccaagggc gccaatgccc aacacctccc ccagcttgcc gagatctcgt ttgcgcccgt
     2041 catcccaggg tatgagcggg tcttccgtgc caagaaaaaa tactatcggc acagacctgc
     2101 ccccggcgca atttgcacca cccctggtta gagtggaagc caccacggcg caagccgcta
     2161 ttttctcagg aatactgagg gagagaagtt gcgtaaagta gccgccattg gaaataccgc
     2221 aggcgtatac gcggcggctg tcgatgttga cggtgcgcga cagtttagcg agcatggctt
     2281 ccacataaga aacatcgtca ttgccggcag tcagaccatc ttgccagcgc ccgccctggg
     2341 cattgggaaa aaccacgaca aaacccttgc ggtctgccac tcccgtcagc ccggacatgc
     2401 cgtacatgag ttgcccgttc atgccaagcc cgtgaaaagc catgaccagc ggataggaac
     2461 gcttgctgtc gtagcccctg ggcacatgga tcaagtaact tcgctctacg ccgccgacat
     2521 tgactgtatc gctaaaatct tgagcagaag cggtctgcgc cgcaggctga gctgacctgg
     2581 actgaacgct ctggggctgt ggctgtgcct gcacggcggt ctgacgcatg ctgccggaac
     2641 cctgactctg tgggtaagcc ggataaccag gttggcgccc ctggtaatac tgcctctgat
     2701 actggctctg atactggctc ccggtctgat tgccggcggg attgacccag cgagcctgcc
     2761 tggagccggg cacccattca tattgcttct gctgagcatc agcccggagc attgcaagac
     2821 tgagcaagag caccagaaac aaagcgttgc cagcacgtct gttcaaaaga cgttctgagg
     2881 agcttccgcc tctgggcgct tcaatttgcc gggtttctct aggatttttt ctaggaatca
     2941 agtgcacccc gagagcttct tcacaagggc ctattgccct tgtaccacac cccagggctt
     3001 tccagccata gccccgagtc cggtcttccg ccggcaggca gcaagtagct agtgatgtcg
     3061 gcggaagatt tacagccgca ttaaactggg tatgtcatag ctgagtttca tggagaatgc
     3121 caaaatgaaa gccccccgct gcaagtttcc caccggtagc cgctcgactc agtccaaatt
     3181 ggctcttgcc gtgcttgccg cttcagcctg cctcctctcc ctgacgccag tctctcccct
     3241 accggcgcag gcacagatgc cgcccggacc ggaagcagtt acggcgccgc ccctggtgga
     3301 aacagcggaa ggtccgctga gggaaaagag acaaaccctg gtgcaaaaga ttcaatcggc
     3361 aaaagaccag ggcattggca ttgcctctta catggcagcc tttaatttca tcgaagaaat
     3421 ggtcaaatcc aaagctgcgg aagccgatat cggcaagaga atagactctc tcgataacag
     3481 cctggaagac cagtttaaac gaagcgctat tctcaaaacc cagagaccga cacccccaat
     3541 agccgaatca tcgccgccgc cgagcgccat gatgggcggc ggcggactgc ctgatctcgg
     3601 aggtggaggc ggtgacatta tctcgaagct gaaaagcaaa tatggtggtc agattccacc
     3661 caatctcaaa gacaaactgc cgcccggtct gcaggaaaaa ttgagcggct tgggtggcga
     3721 cccgagccag cttttgaacg accccaacct gaaggacatc ctcaagggtc tgaagaagta
     3781 gtgaaggaag gtagatgaat tatttcaggg tgtttcgtaa acactccgag atgtcattgc
     3841 tcatcccata gcgcaatgcc aactctagga aatctgatat tgcaccaaac ttatcaccgg
     3901 ctttgaattt agcgcggcat ctgttcttca gggcttgcct ttcaactcgc ttgtccccgt
     3961 aactgagcac ttgatcaaag tcagcaattg ccaggcggta gtctcccatc gatgcataaa
     4021 ccattcccct tgcgcacaaa gaataggggt cccgcccacc tcgcattcct ttggtaaaat
     4081 cggcgagggc accagcatta agacctaaaa cagctctgga ataggctctg cgagtaaagc
     4141 accagaagtt attgggcgcc aggcggcaag cactatcgaa atcagtcagg gcatcaaggt
     4201 cttgctctaa gtcggcgaaa aactcggctc ggtacagata gcctcgataa tcattgggag
     4261 cggcttttat agccatttga aaatgaccga tagcgtttcc atgattccat cgccgccagt
     4321 aaaaccccct agccagacag aaaggaacga ttcgagcctc aatcgcatcg ttatcgccat
     4381 cgccccgata gtcgtacaga agtagctctt cccgggttaa aaaaacaaca tccatagact
     4441 tatctacata cagcctctca agacccaaag ccctttgaaa atcttgagcg gcgcccttat
     4501 cgtctcccag tttcagactt acctgagcac gatttagata gccctcgccg cagtcagggt
     4561 caagggcaat ctgctgtgaa taagctcgca cagaatcttc ataacgcccc agctttgcca
     4621 gaagccagcc cctgcattct tgcaaggcag catgggatgg aaaaacggta acagcctcgg
     4681 tagcggcatt gtaggcttgc tcgatcttat tctcacgcaa gagagctaca acaagataat
     4741 agtggctctc aaactttttc ggaaaccgct tcactgccag tcgagcgtct tttacaaatg
     4801 aaacgcctcc tttcttcaga aacgcaagaa gattgaacaa ggactcgcaa tgatcagcac
     4861 gaagctctat agctgtcctg taggcccgct ccgccagtcc gtctgcgcgc cg
//
LOCUS       DAOQNZ010000092         4898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7268_length_4898_cov_5.474912, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000092 DAOQNZ010000000
VERSION     DAOQNZ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7268_length_4898_cov_5.474912"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1123
                     /locus_tag="PKW73_02535"
     CDS             188..1123
                     /locus_tag="PKW73_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72188.1"
                     /translation="MTSAEKKDASASLNIRIREELKSRLELTASATHKTVSQLVIDIL
                     TQHFHSAGFIDNRIIESPYGRSYEIKTVVSNPHPPTEPTVFFLLHAPWLGGKEVCYYT
                     LGLSISLLRNWRIKDDQRYAAITEIGLALLTFYCNIGKELASLSWNQFHGYLNRRVLY
                     PEDIPHEVESLDQFLNFLNHFSELPWEDTYMNQTITRDYGIWSRNGIFLGYYSGNHVF
                     GKDGIQLGNMITDHTGKKRIYSLAGYYLGDLINNRLLRHSNAGFDGPVPPLERVQQRM
                     AISGEAKKPIDNLPDGYLDEESELVMIPKPQTLTN"
     gene            1210..1545
                     /locus_tag="PKW73_02540"
     CDS             1210..1545
                     /locus_tag="PKW73_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72189.1"
                     /translation="MAAKKSVTFYAAADVAEWIGGLDSGEASRHINDAIREGALKPKS
                     NALFQIPLGFYQMGDLLKILKKAEEEKAADGDNCHSEDPENTEALDAIHAEVKQISDL
                     IAHFEQFVK"
     gene            1553..2227
                     /locus_tag="PKW73_02545"
     CDS             1553..2227
                     /locus_tag="PKW73_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNA72190.1"
                     /translation="MQHYAYIDESGTKDDQEIMTVALVLFEGAFIAHKVHKLLTRELF
                     PNCSKPDKKRKKPSGLHYADMNDKNQNQRLSAGEILAKQSIQCFASCFYHDGTEKSHE
                     QRFEIYTSLIKICLKDALDFHQHLDVTIAQQGNWESYEGPLTSQLDAIVAEQSARLGF
                     RKASFGFESAAKPGIQIADFYAGSIRGHLLKHKDSSLGASFELIEHQVRDIKIHGVDL
                     TAKAKG"
     gene            2510..2662
                     /locus_tag="PKW73_02550"
     CDS             2510..2662
                     /locus_tag="PKW73_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72191.1"
                     /translation="MVWTTIDNSLVTIPIAGLSTNDARPPAMPVKATSNTTISPSDHK
                     ISITLF"
     gene            complement(2820..3341)
                     /locus_tag="PKW73_02555"
     CDS             complement(2820..3341)
                     /locus_tag="PKW73_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72192.1"
                     /translation="MATKLQRTVIVPDTVYAKIYKLAEKEQTNVPFVTRQLLVKALSE
                     EKRPDWLFEIQREIIGLRNDVVAFQRAAEKKERSGEELLLELQKQIADLNQSLADEPE
                     KQDFEPEDDKAHEILLWPELAESPSAIVQEVQEIREAEPVEVVASIEPKEKAKVIRPF
                     FGKLSQLFGSVLG"
     gene            complement(3507..4211)
                     /locus_tag="PKW73_02560"
     CDS             complement(3507..4211)
                     /locus_tag="PKW73_02560"
                     /inference="COORDINATES: protein motif:HMM:NF036314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72193.1"
                     /translation="MATEICKKVCYGNGIRYQSKVQKAKRERNFRTVQLLLDRGGPEL
                     LAQNLVALIDQVRPIDWLCASILGEKTKTPWTIRIHDLGDFGEERYVKAWLLAAKERP
                     FCKLWFYTRSFLEPELFNALTELAALPNCQGWLSVDAENYEAGLLAYAQAPGVWKLAL
                     LQQERTQMEEILPDLVEVAKTKELVSFPVHHGGRHVEPVVAANLYTCPAVLGVYKLES
                     SAAKLRPCQACSFCLP"
     gene            complement(4617..>4898)
                     /locus_tag="PKW73_02565"
     CDS             complement(4617..>4898)
                     /locus_tag="PKW73_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNA72194.1"
                     /translation="PNTDEEKTLWFDVNAWNGLGERALKTITKGREVVLSGRLGLSNY
                     KKEVNGVEVELTKPVIKLTSFHLCGKRPTEDETQSEEKAPAKKTRAAKS"
BASE COUNT         1232 a         1424 c         1004 g         1238 t
ORIGIN      
        1 cactgtatct tgccttacga aaaatcatag atttttgcgt gcggctccgc ttcgctccgt
       61 gacctttgcg catcgctgcc cactcgcgcg ctgcatcttc agccgcaact aaccactcca
      121 accggaactt gacaaaacta caacacctgt attacatttt gcgttacacg cactgcggat
      181 ggtaaaaatg acctcagccg aaaaaaagga cgctagcgcc tccctcaata ttcgcatcag
      241 agaagagctg aaatctcgcc tcgaattaac ggcttccgcc acccacaaga cagtgagcca
      301 gctcgttatc gatatcctta ctcagcattt ccattctgcc ggcttcatcg acaaccgcat
      361 tatcgagtcc ccttacggtc gctcgtatga aataaaaacc gttgtctcaa atccccaccc
      421 tccaactgag ccaaccgttt tcttcctact tcatgctccc tggctcggcg gcaaggaagt
      481 ctgctattac acgctgggac ttagcatctc acttctcaga aactggcgga tcaaagacga
      541 ccaaaggtat gccgccatta ccgaaatcgg tctggctctc cttactttct attgcaacat
      601 aggcaaagaa ctagcctcct tgagctggaa ccagtttcat ggctacctca atcggcgcgt
      661 cttgtaccca gaagacatcc cgcatgaggt agagtccctc gaccaatttc tcaattttct
      721 aaaccacttt tccgaactgc cctgggagga cacatacatg aaccagacaa ttactcgcga
      781 ttacggtatc tggagcagaa acggcatttt ccttggctac tactccggaa atcacgtctt
      841 cggcaaagat ggcattcagc tcggcaatat gattactgac cacaccggca aaaagcggat
      901 ttactccctt gctggctact atctgggcga ccttattaac aacaggctcc tgcgtcatag
      961 caacgccggg ttcgatggtc ctgtgccacc ccttgaacgc gtacagcagc gcatggccat
     1021 aagcggtgaa gccaaaaagc ctatcgacaa cctacccgac ggctacctcg acgaggaatc
     1081 tgagctggtt atgatcccaa aacctcaaac tctaaccaat tgagcctttc tcgctacttc
     1141 cattaagata gatgcacttc taagcggttc tgttataatc aaatagaacc acttagggag
     1201 gactgtccat tggctgctaa aaaaagcgtg acattttatg cggcggctga tgttgccgaa
     1261 tggattggcg gtctcgattc cggcgaggca tcgcggcata tcaatgacgc catcagagaa
     1321 ggggcattaa aaccgaagtc aaacgccctc ttccagatcc cgctcggctt ctaccaaatg
     1381 ggcgatcttt tgaaaatcct caaaaaagcc gaagaagaaa aggcggctga cggcgataat
     1441 tgccattccg aagacccaga gaacaccgaa gccctcgatg ctattcacgc cgaggtcaag
     1501 cagatctccg acctgatcgc tcactttgag cagttcgtca aatagaggga tcatgcagca
     1561 ttacgcctat atcgacgagt ctggaaccaa agacgaccaa gaaatcatga ctgttgctct
     1621 cgtccttttc gagggcgcgt ttatcgctca caaggtccat aagcttctga cgagagagct
     1681 tttccccaac tgctcaaaac ctgacaagaa acgcaagaag ccatcaggac tgcattacgc
     1741 cgacatgaat gataagaacc agaatcaaag actctcagcg ggtgaaatac ttgctaagca
     1801 atccatccag tgttttgcta gctgtttcta tcatgatggc actgaaaaat ctcatgagca
     1861 gcgcttcgag atctatacct ccttgataaa aatctgccta aaagacgccc tggatttcca
     1921 tcaacacttg gatgtaacta ttgcacaaca agggaactgg gaaagctatg aaggacctct
     1981 caccagccaa ctagatgcga ttgttgcgga acaatcagcg agattgggct tcagaaaagc
     2041 ctcttttggc tttgaatcag cggccaaacc tggaatacaa atagcagact tttatgctgg
     2101 ttcaataaga ggtcacctgc tcaaacacaa agattcttca cttggagctt cttttgagct
     2161 aattgagcat caagttcgag atatcaaaat tcatggcgtc gatttaactg ccaaagcgaa
     2221 agggtgagga ccagccccac ccttcctcaa attttctgtt tgcttacttg gagctgagta
     2281 acttaatact ccttcgccgc taattccgca ggggacttca aataagtctt acaacattca
     2341 ctttgtacca caatcatacc aaagctgcct aaaaattcat acagaatttc agtccttcca
     2401 cctcggcggt ggcgcaccgg ctcccctatc agtctctctg ccacccctaa ccttcggcag
     2461 ctttatcgtt cccaccgaca ctttgaagat cacgctcacc agactcagta tggtttggac
     2521 caccatcgac aatagcctgg tcacaatccc cattgccggt ctcagcacca atgacgcccg
     2581 gccacctgcc atgccggtca aagcgacaag caatacgact atcagtccca gtgaccacaa
     2641 aatatccata accctatttt aaatcagccg ccctggcttc gctgctcttc ttcggttgat
     2701 aaaaccggcc tcactactgc cacagacttt ctgtcaccaa cagcccagtc acgaatggca
     2761 aagagagtgt acgcagcgac gctccgcctg cccgaattgt aaatcctacc ctgactgcct
     2821 taaccaagca cactgccaaa aagctgactg agcttgccaa agaacggtct tatgaccttt
     2881 gccttttcct ttggctctat actcgccaca acttctactg gctctgcctc tcttatttct
     2941 tggacttctt gaacgatagc ggacgggctt tctgccagtt caggccagag cagtatctca
     3001 tgagccttat catcctctgg ctcgaagtct tgcttctccg gctcgtctgc cagcgactgg
     3061 ttaaggtcag ctatctgctt ttgcaactcc aacaatagct cttcgcctga acgttccttc
     3121 ttctcagccg ccctctgaaa agcaaccacg tcatttctca gaccgataat ctctctttgt
     3181 atttcaaaca gccagtctgg cctcttctcc tccgaaagcg ctttcaccag tagctgcctg
     3241 gttacaaacg gcacgttcgt ctgctccttt tccgccagct tgtaaatctt cgcgtaaacg
     3301 gtgtccggca caatcactgt cctctgtaat ttcgtcgcca tcgtttcacg ccctctttag
     3361 aagtacgcac taaaaatata ccgaccaggt gacaccctca atatatgttt ttatcatttt
     3421 gtatatccat cgggtttaga cctttccagt cagcagccca tgccatatcc acggctcttt
     3481 aaatatcttt gcaactgcgc tgaccctcat ggcaggcaga agctgcaagc ctggcacggg
     3541 cgcagttttg cggcgcttga ttccagcttg tagacgccta acactgccgg gcatgtatac
     3601 agatttgcgg ccacaaccgg ctccacatgc ctacctccat ggtgtacggg aaaagacact
     3661 agctcttttg tctttgccac ctcaactaaa tctggcaata tctcttccat ctgagtccgc
     3721 tcttgctgta atagagccag cttccagaca ccaggggcct gggcgtaagc cagtaaaccg
     3781 gcttcatagt tttccgcatc tacactcagc cacccctggc aattaggtaa ggctgccagt
     3841 tctgtcaagg cattgaataa ttccggctca agaaagcttc ttgtgtaaaa ccacaacttg
     3901 caaaaaggtc tttcttttgc cgctaacagc cacgctttca cgtaccgctc ctctccaaaa
     3961 tcgcccagat cgtgaatcct tatcgtccag ggcgtcttgg tcttctcccc aagtatcgag
     4021 gcacagagcc agtctattgg tctcacttga tctattaacg cgactaggtt ttgtgcaagt
     4081 aattccggtc cacctctatc taaaagcaac tgcacagtcc ggaaattgcg ctcccgcttc
     4141 gccttttgca ccttactctg gtaccggata ccattcccgt agcaaacttt cttgcagatt
     4201 tcagtcgcca tgacacaagt tttgtccctc ggcatactga acgcccatga cgttttacta
     4261 ttatttgtta gttcaagccc gtaacggttc tcatcgtcac atcgtagttc ttttctcatc
     4321 tcatgcaccc cgtctttaga gcggcttttc gccacttctg gggcacgggg gtggctccgg
     4381 gggtcaagcg gaagtgatca acagttccgc cggcactgac tactcgcttg aggcacggtg
     4441 gtgctacccc cgtagactaa aagaacaaga aaagctacac actggcacat ttcaacagtt
     4501 agaaaaacaa cccaatagca ccagcggcaa agccgccagc caactttgca gtgagaaaaa
     4561 tcagtaaata aagccaaaag aggcagggac tacttagccc ctgccccagg ctcagcctag
     4621 cttttagcgg ctcgcgtttt cttcgccgga gccttttctt ccgattgcgt ctcatcttct
     4681 gtcggtcttt tgccacacag atggaaggac gtgagcttga ttacaggctt ggttagctcc
     4741 acttccacgc cgtttacctc ttttttgtag ttcgacagcc ccagccgtcc gcttagcacg
     4801 acttcgcgcc ctttggttat cgtcttgaga gcacgctccc caagcccatt ccaggcattg
     4861 acatcgaacc acagtgtttt ttcttcgtcg gtattagg
//
LOCUS       DAOQNZ010000093         4871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7342_length_4871_cov_4.148671, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000093 DAOQNZ010000000
VERSION     DAOQNZ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7342_length_4871_cov_4.148671"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="PKW73_02570"
     CDS             <1..1864
                     /locus_tag="PKW73_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72195.1"
                     /translation="LVLREQKALLRLEEAEVGIDWPVVKQRGLSLGLMEELLGFLDAY
                     KKLSATGGSKLEQLKCLKRIERFGEDHNLPVLRIRYMALRVTLEEKLKPVDEEIKREN
                     YSSLINGFIFIGDMPNARLWHRKLALLDEYSKESYPYLCWLDYGIQCGACAFPTSRVL
                     SLEDELMNKPLDKLVGYESGYLQRIQSLYRNAARAAKSKSELESRSLIERANQLQSRI
                     VILESIRADKEAKAFQARQEYLRQSPAERTKLAMAKVQQDIAQADWIAFNLDRGSLLS
                     EWHLLSEGERAYFLSKYLEIARNLIKAHRGDLVSTYLAALHHKASGVDLKSICSSLDE
                     LIAIALQMGDRETAMTIINELVEMASSGQYLENEAAELRKQRELYLSFLREPEVNNVR
                     LANEGQVRELARGFCVKRSILAWQLLRFNDCNNYGPGRKPVKSAVVKFLDTDSERLPL
                     LLENDTAQYRGYARHTMAPFKPSPLPPETASPLNPYALFSLPPGDYKADSLRFSSIRF
                     MSPGRTRIYISGGSICDGPVIWQFSQGSVSTKPYAGGDGGEATLEIWYDGEGTIRFDD
                     RFNFSGLIYAPMARVEMVAPTGGNFKGAIVARDVLVDGNVTIKYDPAFEYLNGH"
     gene            2047..2988
                     /locus_tag="PKW73_02575"
     CDS             2047..2988
                     /locus_tag="PKW73_02575"
                     /inference="COORDINATES: protein motif:HMM:NF018349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HNA72196.1"
                     /translation="MLEPPFLKQLKECKNVLIVGAGGGFDVFTGLPLFFALREAGISV
                     HLANLSFSFRHGEISGRELSRNVVVVTARSRGNEYYFPEGYLAQWLSRKHLDQPIYCI
                     RPTAVKELIESYEILHEHLQFDMMVLVDGGVDSLMRGDESRIGTPLEDMISLSASLQI
                     DVKKQLLCLGFGAETDLCHLYALETVSQLIKARAFLGGVMLSEDMAEVRMFREASEFV
                     FLEMRGYESVICSSIISALEGQFGDHHRTRRTVGSELWINPLMLLYLAFDVAKVTGNI
                     LYFDELTGCQTIEQVRQVIREFRERTAVRPVPPFPLR"
     gene            complement(2997..4622)
                     /locus_tag="PKW73_02580"
     CDS             complement(2997..4622)
                     /locus_tag="PKW73_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72197.1"
                     /translation="MPLFGREPKKPVDFTTLKKPWEAERVSIYEHIVSNLEGDDSEST
                     LNEKGQTLPDEALLYEEDSIRWVAGGLDGAFGHHNDPAEASEFSKALAKAMKQLFKKN
                     SPEALAAFYHLLAPESTLNFIDSFMEYAIAADLTEDPSFYALAKWLLKNAPDREPVKA
                     GIAMLGVCATADDQDLFIDIGKHEEFTLFSCVALLHSLAEPEEALFRLGKAVHGWGRI
                     QVVERLENTTNEEIKAWLLKEGYKNNIMYEYTAYQCATTGDLLAALKQEEPDDELLIA
                     AGNLLSSMNEPGTPLEEYIDAPQATLLLMRHLAKREISISQFLAVNAIKELVLALPET
                     EIKSSRGKPTWAEVSEEVLNICEAMRNRSGWEEKIRAQLKSDNDQDFFDATRLADLFG
                     IDTWEMYFERVKQGKEDWYFLMRTRDESRIEQAVALAEELLPLTEIASGADEKMGMGE
                     GFKAHRCLDFVLQELREWPGHGYKLICTGLKSPVTRNRNLALKALQVWGKDHWQNDTE
                     ALLNELQKDEPNEQTRNYFAEILAQDSHKNNEQ"
     gene            complement(4625..>4871)
                     /locus_tag="PKW73_02585"
     CDS             complement(4625..>4871)
                     /locus_tag="PKW73_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72198.1"
                     /translation="SQSGEFDRCYKEDFSHITNTQRTRHSYYTQSDGAGASEIFAQLF
                     SYAIYKSQVKADEDASDLQITFPRCTKYIKDLIKSNR"
BASE COUNT         1076 a         1095 c         1333 g         1367 t
ORIGIN      
        1 tctcgtgttg cgagagcaga aggccttact gaggcttgag gaagcagaag tcggcattga
       61 ctggccagta gttaagcaac gtggtcttag ccttggctta atggaagaat tgttggggtt
      121 tctcgatgct tacaaaaagt tgtcggctac tggcggcagt aagctggaac agcttaagtg
      181 tctgaaaagg attgagcggt ttggagaaga tcacaatctg ccagttctca gaattagata
      241 tatggcactg agagtgacac tggaggagaa gctcaaacca gttgatgaag agattaagcg
      301 tgaaaattac tcctcgctga taaatggatt tattttcatc ggagacatgc ccaatgccag
      361 gctctggcat agaaagctag ctttacttga tgagtattct aaggagagtt atccttattt
      421 gtgttggcta gactatggta ttcagtgtgg tgcctgtgcg tttcccacca gcagagtgtt
      481 gagtcttgaa gatgaactca tgaataagcc gctggataag cttgtgggtt atgagtccgg
      541 ttacttgcaa cgcattcaga gtctttatag aaatgctgct cgggctgcta agtcaaaatc
      601 tgaattagag tccaggagcc tgatagagcg agcgaaccag ttgcaatcga ggattgttat
      661 tctagagagt attagggctg ataaagaggc aaaagctttt caagccaggc aagagtattt
      721 aaggcaatca ccggccgagc gtactaagct cgccatggcg aaggtgcagc aagatatagc
      781 tcaagctgac tggattgctt ttaatttaga tcgaggttct ttgctgtcag agtggcatct
      841 attgtcggaa ggagagcgag cgtattttct ttccaagtat ttagagatag ccaggaatct
      901 tatcaaagca caccggggag atttagtgag cacctatctg gccgccctgc atcataaagc
      961 ttccggagta gatctaaaat cgatatgcag cagtcttgat gaattgatcg ccatcgcatt
     1021 gcagatggga gatcgcgaga cggccatgac catcatcaat gaacttgtcg aaatggcttc
     1081 ttctggtcaa taccttgaga acgaggctgc tgaactgaga aagcagagag agttatattt
     1141 gtcctttctg cgcgagccgg aagtgaataa tgtgcgactt gcaaacgagg ggcaggtgcg
     1201 cgaactggcc cggggcttct gcgtgaaacg tagtattttg gcctggcaac tgcttcgttt
     1261 caatgattgc aataactatg gccctggccg aaagcccgta aagtctgctg ttgtgaagtt
     1321 tctcgatacc gacagtgaga gactgccgct acttctggag aatgacactg ctcaatacag
     1381 agggtatgct cgtcatacaa tggcaccatt taagccttct ccgctgccac cggaaactgc
     1441 cagccctctg aatccgtatg ctctttttag cctgcctcct ggtgattata aagctgatag
     1501 cctgcgtttc agctctatcc ggtttatgtc tccgggacgg actcgtattt atatttcagg
     1561 cggcagtatc tgtgacgggc cggtaatctg gcaattcagt caggggtcgg tgagtacaaa
     1621 gccttatgcg ggaggggacg gtggtgaagc cactcttgag atctggtatg acggtgaagg
     1681 gactattcgt ttcgatgatc gcttcaattt cagcgggctc atctacgcac ccatggccag
     1741 agtggagatg gttgcgccaa ccggcggtaa ctttaaaggt gctatcgttg cccgggatgt
     1801 tctggtggat gggaatgtga ccattaaata cgatccggcc ttcgagtatt taaacggaca
     1861 ttagggcgcc ttctgggacg aattatctcc ccacggatag gtttgccgga aaagtaaaga
     1921 gtctttgagc gttatagcaa tagataggat tgctccagac cattaagttt ttgcaggcac
     1981 ccattcagtt ttcggtagac cgcaatggca gtcgggtata gaggttttgc ctgaccaggt
     2041 gatcaaatgt tggaaccgcc ctttctcaag caattgaaag agtgcaagaa cgttctcatt
     2101 gtgggagcgg gcggcggctt tgatgtattc accgggttgc cgctattctt tgccttgcga
     2161 gaggcgggaa tctccgtgca tctggcaaat ctctcgtttt cctttcgcca tggtgagata
     2221 agcgggagag agctcagtag gaatgttgtg gtggttaccg ccagatcccg tggtaacgaa
     2281 tattatttcc cggagggcta tttggctcag tggctcagcc gcaagcacct agatcagcca
     2341 atttattgca ttcgcccgac ggctgtaaaa gagctgattg aaagctatga gattctgcac
     2401 gagcatttgc agtttgacat gatggttctg gtggacggcg gtgtagattc tttgatgcgt
     2461 ggtgatgagt cgcgcatcgg tacgcccctg gaagatatga tcagcctgtc ggcttccctg
     2521 cagattgatg tgaagaaaca gttgctttgc ctgggctttg gtgcggaaac cgatctctgc
     2581 catttgtatg ccctggagac ggtatcgcag ttaatcaagg ctagagcatt tctgggcggc
     2641 gtcatgctca gtgaagacat ggcggaagta agaatgttca gagaagcctc cgagtttgtt
     2701 ttccttgaga tgcggggcta tgaaagcgtc atttgcagct cgattatttc cgcgctcgaa
     2761 gggcaatttg gggatcatca tcgtaccagg cgcaccgtcg gcagtgagct ctggataaac
     2821 ccgctcatgt tgctatatct ggcctttgat gtggcgaaag taaccggcaa cattctctac
     2881 tttgacgagt tgaccggctg ccagactatt gagcaggtgc gccaggttat tcgcgaattt
     2941 cgtgagagaa cggcggtgcg acccgtgccg cccttcccgc tcagataaat cttgctctac
     3001 tgttcgttgt ttttatggga atcctgggcc aggatttcag caaaataatt tctggtttgt
     3061 tcattgggtt cgtctttctg aagttcattc aagagagctt ccgtatcatt ttgccagtgg
     3121 tcttttcccc aaacctgcag cgctttcaaa gcgaggttac gatttcgggt gaccggactt
     3181 ttcagtccgg tacaaattag tttgtagcca tggcctggcc attcgcgcag ttcttgcaag
     3241 acaaagtcta ggcagcgatg agctttgaag ccttctccca tgcccatctt ttcgtctgcg
     3301 ccgctggcga tttcggtaag cggcagcagt tcttccgcca gtgctaccgc ttgctctatg
     3361 cggctctcgt ctctggtgcg catgaggaag taccagtctt ccttgccctg ttttactcgt
     3421 tcgaagtaca tttcccaggt atcgataccg aagaggtcgg ctagtctggt ggcatcaaag
     3481 aagtcttgat cgttatctga tttgagttgt gctctgattt tctcttccca gccggagcga
     3541 ttgcgcatgg cttcgcagat attgagcact tcttcgctga cttccgccca ggttggtttt
     3601 ccccggctgc ttttaatttc agtctcgggc aatgccagca ccagctcttt gatggcgttt
     3661 accgccagga actgactgat ggaaatttct cttttggcca agtgccgcat taataggagt
     3721 gttgcttgcg gtgcatcgat gtactcttcc aggggcgttc ccggttcatt catgctcgat
     3781 agaaggttgc cggcagcaat taatagctcg tcgtccggct cctcttgctt gagcgccgcc
     3841 agtagatcac ctgtggtggc gcactggtaa gcggtgtatt cgtacatgat attgttcttg
     3901 tagccttctt tgagaagcca ggctttgatt tcttcgtttg tggtattttc caggcgttcg
     3961 accacttgaa tgcgtcccca accatgcacg gctttgccca ggcgaaaaag agcttcttcc
     4021 ggttctgcca ggctgtgcag cagggccacg caggagaaaa gggtgaattc ctcatgtttg
     4081 cctatgtcta tgaagaggtc ctggtcgtcg gcggtggcgc aaactcccag catagcgatg
     4141 ccggccttga ccggttctct gtccggggca ttctttaaga gccatttggc aagcgcgtaa
     4201 aaggagggat cttcagtcaa gtcggcagcg atggcatatt ccatgaagga gtcgataaag
     4261 ttgagggtgc tctccggtgc cagaagatgg tagaaagcgg ctagagcctc cgggctgttc
     4321 tttttgaaca gttgcttcat ggctttggcc agggctttgg aaaactcgct tgcttctgcc
     4381 ggatcgttgt ggtgtccgaa ggcgccgtct agaccgccgg ctacccagcg aatactatct
     4441 tcctcgtata acagtgcttc gtccggtaag gtttgaccct tttcgttcaa ggttgactcg
     4501 ctgtcatcac cctcaagatt actaactatg tgctcgtata tgctaacgcg ctcggcttcc
     4561 cacggttttt tcagggtggt aaagtcgact ggttttttcg gttctcttcc aaatagtggc
     4621 atgtttagcg gttactttta atcaggtctt ttatgtattt tgtgcagcgc ggaaaggtga
     4681 tttgcagatc gcttgcatct tcatctgctt ttacttgcga cttgtatatg gcataactaa
     4741 agagttgggc aaagatctcg ctcgctcctg ctccgtccga ttgcgtgtaa taggagtgtc
     4801 tggtgcgctg ggtgttggta atgtgcgaga agtcttcctt gtagcatcgg tcaaattcgc
     4861 ccgactgcga c
//
LOCUS       DAOQNZ010000094         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7347_length_4868_cov_4.208394, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000094 DAOQNZ010000000
VERSION     DAOQNZ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7347_length_4868_cov_4.208394"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /locus_tag="PKW73_02590"
     CDS             complement(<1..1882)
                     /locus_tag="PKW73_02590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA72199.1"
                     /translation="MTSVKEPESLKEPSEEPGKAKDDSQASFAETAMQLAGKSEEEAK
                     RTGAVDRADDQVEALFAEQYKTSNSPVHKAVWDAKIPLELFCPPKMPDGALSLPSMQK
                     SLDVLRKHKKAGTMMDANGKIAAGVFTDLAQAGYWGMLIEEKYGGQGATVRQFMTFLT
                     RVASIEPTAAGLASVHGCIGAVDPVRSFGSEEQRKRILPKLASGEALSGFALTEPGAG
                     SDLTALKTKAELVGDHYVVNGEKLFITNAVPGRTVGLVCLVNNKPAALITELPEKEDE
                     HFQVVPYSLHALKHAFNNGLKFKDFKVPKENLLVPTAGDGLTVAYHGLNMGRLALCAT
                     ADGVMRIMLANMLPWAKFRQTYGQAIETRELVKRRIARAAALIVGAEALVAWGSWLLD
                     QGFRGELECIIAKIFGSEAEKECAIELFMKTHGGRSFLSGHLLGDNIHDFLAPCIYEG
                     EGEMLGMAFFKSLAKQHGVAYFEPIGKKVAQAGIKVFNPFNPAHAFALRNELIPYSMW
                     WIGKQLEGVDQHEVPGMDADLKAHVDYALEFLSESPLELSQNMVKHQLKLADRQCRIA
                     EMSSRIQDAVIILVTSLYAHAEQNPTLIAAADILCQDLRRKLTGERPSDSYFKAAGKL
                     ADM"
     gene            complement(1995..3212)
                     /gene="rnd"
                     /locus_tag="PKW73_02595"
     CDS             complement(1995..3212)
                     /gene="rnd"
                     /locus_tag="PKW73_02595"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNA72200.1"
                     /translation="MTNDKTSAREKHGKLIDAQEDLREMLQSIEACGIFGIDLEFIPE
                     KSYFPLICLVQVAVGERVFLVDPIKVQNLDDLWRYVADPGYIKVLHAGSQDLAIVNQR
                     SGFIPKRIFDTQIAAGFLGLGYPAGYGKLLHALFDLTLSKTESFTDWSLRPLTASQID
                     YAIDDALHLLPLYQALEERLKERGRLDWVLEECKLYEQESYYQKETGREFMRIKGAQG
                     LSRRKLAVLQAICLWRDQEARRIDKPPRFVLSDNIILELAKKPPTTPGDISRIRGIKD
                     GQLSGYGKHIIRVVAEALALKEADCPQWPAGRAPSKADVLIADVLYTVLKVRSQEIEI
                     APELIATRDELQRFVRQDKGQLEKEEEESALTSGWRHKLAGSELHAILSGAPLTVTVT
                     STSHNPISIKLEN"
     gene            complement(3205..4086)
                     /locus_tag="PKW73_02600"
     CDS             complement(3205..4086)
                     /locus_tag="PKW73_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNA72201.1"
                     /translation="MQESSQTEPLTGEDIDFVSKLTEEAGHLALSMRDRLEISEKTSP
                     EDQVTSADLAISRLFKERLQSRFRDDAVISEEDLDHALNHERKRIWLIDPIDGTQNYI
                     MDHDDYCIMVGLLIDLEPCFGWVLAPQSGRLYYGGKGFGVKRRQTDCHPHAMQGKAGA
                     LPLGETLLEEPPPLPLNSSARVVMGTRDRRSNPWVKDLEKVDLVKTGSIGLKVALILE
                     GEADVFAHLSGKLKTWDTAGPAALALGAGLDVGGLELDSLDFPLPETKQECTVIMGRK
                     GALAWCRKHLQGPQSND"
     gene            complement(4117..4593)
                     /locus_tag="PKW73_02605"
     CDS             complement(4117..4593)
                     /locus_tag="PKW73_02605"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNA72202.1"
                     /translation="MGIFGAQKVNVVSAQEAKDMVEEYFAKRGLVAADHVLADAGGLG
                     WWLMEGSARVYILVQEGPKDTRGKANTLVRITAPLVYLPETGRELFYRRLLDLNGNLS
                     LCALSTHDDVVLVVAQRPTTGLDQEELDDMVWHVAYVADLLDNKLAEEYGCKMYRN"
     gene            4791..4862
                     /locus_tag="PKW73_02610"
     tRNA            4791..4862
                     /locus_tag="PKW73_02610"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4823..4825,aa:Asn,seq:gtt)
BASE COUNT          980 a         1303 c         1342 g         1243 t
ORIGIN      
        1 ccatgtcggc cagtttaccg gctgctttga aatagctgtc ggaaggtctt tcgccggtca
       61 gcttccgtct caagtcctga cagagtatgt cggcggcagc gatcaaagta ggattttgct
      121 cggcgtgcgc ataaaggctg gtgaccagga tgatcacggc atcttgaatg cgcgatgaca
      181 tctcggcgat gcggcactgt ctgtctgcca gtttgagctg gtgcttgacc atgttctgtg
      241 aaagctcgag gggagactcg gagaggaact ccagggcgta gtccacatgg gccttcaggt
      301 cggcgtccat gcccggcact tcgtgctgat ctacgccttc caactgcttg ccaatccacc
      361 acatggaata ggggattaac tcgttgcgca aggcaaaggc atgggccgga ttgaaaggat
      421 tgaaaacctt gatgccggct tgtgctactt ttttgccgat gggttcgaag taggccacgc
      481 catgttgttt ggccagagac ttgaagaagg ccatacccag catttctcct tcgccttcat
      541 aaatgcaagg ggccaggaag tcatgaatgt tatcgcccag gaggtggccc gagagaaagg
      601 agcggccgcc gtgggttttc atgaatagct cgatggcgca ttctttctcg gcctcagaac
      661 caaagatttt ggcgatgatg cactccagct cgccgcggaa accctggtct agcaaccagg
      721 aaccccaggc taccagcgcc tcggcaccaa caatcaaggc tgctgctctg gctatgcggc
      781 gcttgaccag ctctctggtt tcaatggctt gtccgtaggt ttgtctgaat ttggcccagg
      841 gcaacatgtt ggccagcatg attctcatta caccgtcggc tgtggcgcaa agtgccagcc
      901 gtcccatatt cagtccgtgg taggccacgg ttaagccgtc accggctgtg ggaaccaaca
      961 ggttctcttt gggtaccttg aagtctttga acttgagacc gttattgaag gcatgcttga
     1021 gggcgtgcag tgaatacggc acaacctgga agtgctcgtc ttctttctcg ggcaattcgg
     1081 tgataagggc tgccggttta ttgttgacaa ggcagaccag acccaccgtg cgtcccggca
     1141 cggcatttgt gataaacagc ttctcgccat tcaccacata gtggtcccca accagttccg
     1201 cttttgtttt gagagcggtg agatctgagc cggctcccgg ctcggtcagg gcaaatccgg
     1261 aaagagcttc accgcttgcc agtttcggca gaattctctt acgttgctct tcgctgccga
     1321 agctgcgcac agggtcgaca gcgccgatac agccgtgtac ggaagccagt ccggctgccg
     1381 tcggctcgat ggaggccacc ctggtcagga aggtcatgaa ttggcgtacg gtggcaccct
     1441 gaccgccgta tttttcctcg atcaacatgc cccagtagcc ggcctgggcc agatccgtaa
     1501 aaacgccggc agcaattttg ccgtttgcat ccatcatggt tccggctttt ttatgcttgc
     1561 gcagcacgtc cagcgatttc tgcattgacg gcaagctgag ggcgccgtcc ggcatttttg
     1621 gtgggcaaaa gagttcaagg ggaattttgg cgtcccagac tgctttgtgc acagggctgt
     1681 tggaagtttt gtattgctcc gcgaagagcg cctctacctg gtcatcggcg cggtccacgg
     1741 caccggtgcg cttggcttcc tcttccgact tgccggcaag ttgcatggcc gtttccgcaa
     1801 agctggcttg actgtcgtcc ttcgctttgc ccggctcttc actgggttcc ttcagggatt
     1861 ctggctcttt tacggatgtc atataaaccg ccttagattg accttttaag ggcttcaaga
     1921 ttagtttaga ttctactaga cttggcggct gcttatgatt aagagctaat tttcttgaag
     1981 tttgaggaag gcgctcagtt ttccagcttt atggaaatag gattgtggga agtgcttgtc
     2041 actgtcactg tcagtggtgc gccgcttaag atggcgtgta gttcagaccc tgccagttta
     2101 tggcgccagc ctgaagtgag agcactttct tcttcttcct tctccagttg acccttgtcc
     2161 tgacgcacga agcgttgcag ttcatctctg gtggcgatta gctcaggagc gatttctatc
     2221 tcctgggaac gtaccttcaa aaccgtgtac agaacatcgg caatgagaac atcggctttg
     2281 gatggggctc tgccggccgg ccactgggga caatcggctt ccttgagcgc cagcgcttcc
     2341 gctaccaccc tgatgatatg tttgccatag cctgaaagct gcccatcttt gataccgcgg
     2401 atgcggctga tatcgcccgg tgtggtgggc ggcttctttg ccagttccag aatgatattg
     2461 tcggacagca caaagcgcgg gggcttatct atgcgccggg cctcctgatc gcgccagagg
     2521 cagatggctt gcagaacggc cagttttcga cgggagagac cctgcgcccc tttgatgcgc
     2581 ataaattcgc gtccggtttc cttctgatag taactttcct gctcgtaaag tttgcactcc
     2641 tccagcaccc agtccagtct gcctctctct ttcagccttt cttccagggc ctgatagagc
     2701 ggcaacaaat gcagtgcgtc gtcgatggcg taatctattt gcgaggctgt aagcggtctc
     2761 agggaccagt cagtgaaaga ttcggtttta gagagggtca ggtcaaacaa ggcatgcagc
     2821 aacttgccgt agccggccgg ataaccaaga ccgagaaagc cggcggctat ttgtgtatcg
     2881 aaaatccgct tggggatgaa accggaacgc tgattgacga tggccaggtc ttgagagccg
     2941 gcgtgcaaga ctttgatgta gccgggatcc gccacatagc gccagaggtc gtcgagattc
     3001 tgaaccttga tgggatcaac caggaagact cgctctccta ccgctacttg cactagacaa
     3061 atgaggggga aatagctctt ctcaggaatg aattccaggt cgatgccgaa gatgccgcaa
     3121 gcctcgatgc tctggagcat ttccctgagg tcttcctgcg cgtcgatgag cttaccgtgc
     3181 ttttctctag cggaggtctt gtcgttagtc attggattgg ggtccttgta aatgtttgcg
     3241 acaccaggcc agggctcctt ttcgtcccat gatgacggtg cattcttgtt tagtctcggg
     3301 caggggaaaa tccaggctgt ccagttccag tccgcccaca tccagtccgg cgccaagggc
     3361 cagggcggcc ggtcccgccg tatcccaggt ctttaatttg ccagacaaat gagcaaaaac
     3421 atcagcttca ccctccagaa tcagtgctac tttcagtccg atgctgccgg ttttgaccag
     3481 atcgactttt tccaggtcct tgacccaggg attggagcgg cgatctcttg tgcccatgac
     3541 cactcgagcc gatgagttga gaggtagcgg cggcggttct tccagtaaag tctcaccgag
     3601 cggcagagcg ccggcttttc cttgcattgc gtggggatgg caatcagtct ggcggcgctt
     3661 cacgccaaag cctttgccgc cgtagtagag tcggccgctc tgaggggcca gaacccagcc
     3721 gaagcagggc tctaagtcaa tgagcagacc aaccatgatg cagtagtcat catgatccat
     3781 gatgtaattc tgggtaccgt ctatgggatc tatgagccag attcgctttc tttcatggtt
     3841 gagtgcgtgg tccaggtctt cttcggaaat aacggcgtca tcccggaagc gactctgcag
     3901 cctttcctta aacagcctgg agatggccag gtcggcgctg gtgacctgat cttcagggct
     3961 ggttttttcg ctaatctcaa gcctgtctcg cattgaaaga gccagatgac ctgcttcttc
     4021 cgttagtttc gagacaaaat cgatatcttc cccggtcagg ggttcagtct ggcttgattc
     4081 ttgcaatgca gttactttct ttggcaatgc ggcaggctaa ttccggtaca ttttgcagcc
     4141 gtactcttca gccagtttgt tatcgagcag gtcggctaca taggccacat gccagaccat
     4201 gtcatccaat tcctcttgat ccagtcctgt tgtcggtctc tgggcgacca ccaggaccac
     4261 gtcatcgtgg gtgctgagag cgcacaggga gagattgccg ttgagatcga gcaggcgccg
     4321 atagaatagc tctctgccgg tttcaggcag ataaacgagc ggggcggtga ttctcaccag
     4381 ggtgttggcc tttcctcttg tgtccttggg tccctcttgc accaggatgt agactctggc
     4441 gctgccttcc atgagccacc agcccagacc gccggcatcg gccagtacgt gatcggcagc
     4501 caccagacca cgtttggcga aatactcttc caccatatcc ttggcttcct gggcggaaac
     4561 cacgtttact ttttgtgcac cgaatatacc catagaccta tattcccgta ttcccgcttt
     4621 tgcttttgcc ttattgctaa ctcagccctg aggtggagcg agttccaagt atacccaatg
     4681 gtaaggcggc tgagtttatt aagcgcgctt aataaacatc tacgtaaagg aaacgttttc
     4741 aggtagttct cattggagtc tcctggtgtt actatttttc tacactactt tcccgagtag
     4801 cgcagcggta gcgcaggtga ctgttaatca cttggtcgct ggttcgaatc cagcctcggg
     4861 agtttttc
//
LOCUS       DAOQNZ010000095         4852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7390_length_4852_cov_3.490723, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000095 DAOQNZ010000000
VERSION     DAOQNZ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7390_length_4852_cov_3.490723"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..939)
                     /locus_tag="PKW73_02615"
     CDS             complement(307..939)
                     /locus_tag="PKW73_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72203.1"
                     /translation="MCEIDLQAMYVAASLVPAEHVPDTFNVTGVSVNYLNYMDSLTRI
                     VEAFEADRQILVGDFVPFTRTGIKTRFPQLESVSVQWNTGPRQGGAYWYTEELWLGEW
                     TLPGFLVMSDGVIHAGDSCARESFLEYWKGNASFRCLGANASIKADAVREGEIWSGLK
                     KFVVFYRHKGVHPTYYGEDRWRRHPDPEPFWSHFVVRAGSDKLFGQLFEK"
     gene            complement(1176..1634)
                     /locus_tag="PKW73_02620"
     CDS             complement(1176..1634)
                     /locus_tag="PKW73_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72204.1"
                     /translation="MREDMKYLMLDRPRVNHKNRQCKGEKRKAQRGLEFLPLHEPMKR
                     RFDFHCRNKGTRLVGREDHRKQFSDYYAPILRFLRSRVGRKWDDVNSEIRREADSKTV
                     LGFHLVFHVKCLVRRSVYLVDGRAYSTKHNELTKGEFYVHPESGLLCRVA"
     gene            complement(1742..2629)
                     /locus_tag="PKW73_02625"
     CDS             complement(1742..2629)
                     /locus_tag="PKW73_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72205.1"
                     /translation="MKTQNRVNKYPVLTVAMMNTLFMELLNRKQQIANFVGVNLVKVE
                     PSRQAQLLQALAMCLHADPLLEKCESAIAYLEAPAADETPVNSEPVAEGKEAPDELAA
                     AIGTIFSGLAGPTVNLVDGVCEALAEQFGVSSEAVPPFLVQTNSYMLGFIGKMFDSAE
                     AQLEEDAVTSLVSIPEMSSRLEAAVAAYEDLHSRLAEVELSEVSVEQQVRFLFVTALV
                     QLGASLLSQASPAAEYLCQEGDKVTVEASISRAVVRQYGVNEAAVAPFLHGVNSLILP
                     WIEELAQEASAVFETTDSQ"
     gene            complement(2765..3427)
                     /locus_tag="PKW73_02630"
     CDS             complement(2765..3427)
                     /locus_tag="PKW73_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNA72206.1"
                     /translation="MQDVNSGSTAYLDGSQAFKLMELNGELEFSLAVPTSTVIGYRPE
                     APHMICVLEHAQKHNGRLTLPGGKRTGADTHLQCAINEFDQEVGGSGASLFDIELLWV
                     KADPLADVRRVSLAKATDNRCPQSMKNTGVTAFYGYPDYIYRAAVSGEPSPKDGEAKS
                     VRWFDINDLVIEASPEASIFGAQHDLIIYQYRKYLQGGLVSSVVFADLAIMRSEILAS
                     RA"
     gene            complement(3494..3802)
                     /locus_tag="PKW73_02635"
     CDS             complement(3494..3802)
                     /locus_tag="PKW73_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72207.1"
                     /translation="MKNHNLAIITGFALNVAGADSLSLAVSVELADGTTGIVNCENLP
                     AKELNDRIAGLLKVAGVVSTDKLVDAEVRVQVQDGQILALGHRLQARWWQSATLKLAA
                     "
     gene            complement(4310..>4852)
                     /locus_tag="PKW73_02640"
     CDS             complement(4310..>4852)
                     /locus_tag="PKW73_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72208.1"
                     /translation="PADGLSALLTIGYTRQYVMENIEQGVSFHLLAFAGGPRPRLATW
                     SNVLLSAGQVYPELRRHFAASASYLPSLTVSEVEKRCGFALAPVHAKGVADERFITLE
                     RLLAVDPSDGAVPVLLRRFLYHCCRLSELFSGDGYTRIEAGPRPVEANGRPYEGIKEY
                     VMPNSVILELQPLFWRELRI"
BASE COUNT         1279 a         1356 c         1166 g         1051 t
ORIGIN      
        1 tgttcggcgc ctatgaagaa accgtacacg tcgaaatcgg aagtgactga tgcagtgccg
       61 aaagcatgac tgcccatcag cacttcatag cgggcgctgt tcaataaata ggcaggagcg
      121 ctaatgtgtc cggcctctgt cattttcagt aagatgctca tgtgacctcg gtgaaagcat
      181 tggcaaatag cgggtccttg cgcttgattt gccttgttga gcggagcatc caggctcggc
      241 taaaaaattg aagtgcagcg tccctaccac aacagtccta tactgccttc gacttccgtc
      301 ttccggctac ttctcaaaga gctgcccaaa caacttatcc gaacctgccc tgaccacaaa
      361 gtgcgaccag aacggctcag gatcaggatg acggcgccaa cggtcttcac catagtaagt
      421 cggatgcacg cccttatgcc ggtaaaagac cacgaacttc ttgagaccgc tccagatttc
      481 gccctccctg acggcatctg ctttgatgga agcattcgct cctaagcacc ggaaagacgc
      541 attgcccttc caatattcca agaaactctc tctggcacag gagtctcccg catgaatgac
      601 accgtcactc ataaccagaa aaccaggcaa agtccattct cccaaccata gttcttccgt
      661 gtaccaatag gcaccgcctt gtctgggccc ggtattccat tgcaccgaca cactttccag
      721 ctgggggaag cgcgttttga taccagtgcg agtgaaggga acaaagtcgc cgaccagaat
      781 ctggcggtct gcctcgaaag cttccactat tctggtcagt gaatccatgt agttcaaata
      841 attgaccgaa acaccggtaa cgttgaacgt atcaggcaca tgctcagccg gtaccaggct
      901 ggcagctacg tacattgctt gcaaatctat ttcacacata aaatactcca acttgaaatt
      961 gacgaataaa ataaagcgct cagacacgaa gaaagcggct gctacaaagc acatccctat
     1021 gatcttttga atagctatct agtcgctgaa ttacctgaaa caccatcagg gcgtcgaggc
     1081 aattcaaaag aacaagcgag ctatgctcca taactttcca caaactccgc cggggcggag
     1141 tggaagccga actggaggct tccactcaca cataatcagg caactcgaca gagcagaccg
     1201 ctttccggat gcacatagaa ctcacctttg gtcagttcat tgtgcttggt gctgtatgct
     1261 cttccgtcga ccagataaac gctgcggcgc accaggcact tcacatggaa gaccagatga
     1321 aagcccagta cggttttaga atcagcctcc cggcgaatct ccgaattgac gtcgtcccat
     1381 ttgcgcccga ctcttgaacg caggaatctc aaaataggcg cgtaatagtc gctgaactgc
     1441 ttacgatgat cttcgcgacc cacaaggcga gtgcctttgt tgcggcagtg aaagtcaaag
     1501 cggcgcttca tcggttcgtg caagggaagg aactccagac cacgttgcgc ctttcgtttc
     1561 tcacccttgc actggcggtt tttgtggttg acacgtggtc tatccaacat cagatacttc
     1621 atatcttctc tcatgacaca ctccgagtaa tttttaaatg taagagatag caacccgctc
     1681 accagatgga gagcgggaaa ccgtcattca agcccttgga acaggcgttg cttgcctgtc
     1741 gttactggct atcggttgtt tcaaaaactg cactggcttc ttgcgccaac tcttcaatcc
     1801 agggcaagat aaggctatta acaccgtgga ggaagggcgc cactgccgct tcattcacac
     1861 cgtactgtcg cacaacggct cgacttatgg acgcctcaac ggtcaccttg tcgccttcct
     1921 ggcacaggta ttcggcagcg ggagaggctt gcgaaagcag gctggctcca agctgcacca
     1981 gggcagtgac aaacaaaaag cgcacctgct gctccaccga aacctccgaa agctccacct
     2041 cagcaagcct ggagtgcagg tcctcatagg cagccactgc cgcctccagg cgactgctca
     2101 tctccggtat ggataccagg ctggtgactg catcctcttc cagttgcgct tcggcactgt
     2161 cgaacatctt gccgatgaag cccagcatgt agctattggt ctgaacaagg aaaggtggaa
     2221 cagcctcact gctgacaccg aactgctcag ccagggcttc acaaacacca tccacaagat
     2281 tgacggtagg ccctgccaga ccgctaaaga tagtcccaat ggcggctgcc aactcgtccg
     2341 gagcttcctt gccctcagcg accggctccg aattcaccgg agtttcgtcc gccgcaggag
     2401 cctccagata agcaatggca ctctcgcatt tttccagcaa gggatcggca tgcaaacaca
     2461 tagccagcgc ctgcagaagc tgcgcttgcc gggacggctc caccttaacc aggttaacac
     2521 cgacaaagtt cgcgatttgc tgcttgcgat tgagcagttc cataaatagc gtgttcatca
     2581 tggcaacggt gagaacaggg tatttgttca cgcgattttg agttttcata gttagcctca
     2641 agctattttt tggtcaaatg aatgtcaggt aaaggcacga aagtgccgcc accattaaaa
     2701 aacgtccctg ttcaaaatcg tcctgaaccg gctgcaacac caggggaagc ctggcctcag
     2761 ccaatcaagc ccgggaagcc agtatctcac tgcgcataat cgccaggtcg gcaaacacga
     2821 cggaggaaac gaggccgccc tgaagatact tgcgatattg atagatgatc aagtcgtgct
     2881 gagcaccgaa gatagaagct tccggcgaag cctcaatgac cagatcgttg atatcaaacc
     2941 accgtactga tttagcttca ccgtctttgg gagatggttc gccggatact gcggctcgat
     3001 aaatgtagtc cggatagccg tagaaggcag tcactccagt attcttcatc gactgcggac
     3061 aacgattatc cgtggctttg gccagactga ctcgcctcac atcggcaagg ggatcagctt
     3121 tcacccaaag caattcgata tcgaagagac tggctccaga gccacccacc tcctgatcga
     3181 actcattgat ggcgcattgc aagtgcgtat cggcaccggt gcgcttgcca cccggcagag
     3241 tcagacgccc attgtgtttc tgagcatgct caagaacaca gatcatatgg ggtgcctctg
     3301 gtcgatagcc aatcaccgtg cttgtgggaa cagccaggct gaactcaagc tcaccgttta
     3361 attccataag cttaaaagct tgggaaccgt ctaagtaggc ggtagacccg ctattaacat
     3421 cttgcataaa ccactcctgg taatggtgga tgaaagaagg ccacaagtgc cttcaccaac
     3481 aaggacaacc cgctcaggcc gccaacttca aggtagcaga ctgccaccat cttgcttgca
     3541 ggcgatgacc gagggccaga atttgaccat cttgcacctg cacacgtact tcggcatcaa
     3601 ccaacttgtc ggtgctgacc acgccggcca ctttcaaaag tcctgcgatg cgatcgttca
     3661 actccttcgc gggaagattc tcgcaattga cgatgccggt agtaccatct gccagttcaa
     3721 cggaaactgc gaggctgaga ctatcggcgc cggctacgtt aagtgcaaaa ccagtgatga
     3781 ttgctaagtt atgatttttc atggcttact ctgaagtaaa caaagttagt aaaagtgatg
     3841 ttagccccgc taagttctgc cgacccgata aatcctgatt ttccaaagaa gctcttccca
     3901 gagcagtaaa ctgcctgagg aaaaaacaat gtggaaagcg ccgaaaaggc ttttcagtaa
     3961 tttagatgcg gctccagaca aaaacaaaag gtagggtgac taaaccatag cgatagacgt
     4021 accggaaaat cgattacgcg gcgggacact ttaatgtgcc gaattatcca ttccagcccc
     4081 gaatgaaaac gtgaaaaggt tatttatggg ggcttttaac gcctttaaaa ggaaattttt
     4141 gaggctattt ttgccaaacc ttttctacag gacggagagc aaaccacctt accaaacgct
     4201 caccgtccca ccaaacacat ctagagcgtc ctgtttttag ccacagttcc gacaccgcta
     4261 gcgcgctgtc ttcaaatgcc ctggccaagc caggcgcctc atcatcgcct taaattctca
     4321 gctcccgcca gaaaagaggt tgcagttcga ggatgacact attgggcatg acatactctt
     4381 taatgccttc atagggtctg ccgttcgcct ctaccgggcg aggtccggct tctatgcggg
     4441 tatagccgtc gccgctgaag agttcggaga ggcggcaaca atgataaaga aaacgacgca
     4501 gcaaaaccgg cacggcccca tcagaaggat cgactgccaa aaggcgttcc agcgttatga
     4561 aacgctcgtc ggcaacaccc tttgcatgca ccggcgccag agcgaaacca caacgcttct
     4621 ccacttcgct caccgtcaaa gaaggtaagt aggaagcaga ggcagcaaag tgccgcctca
     4681 actccggata gacctgaccg gcactcagaa ggacattgct ccaggtagcc agacgcggac
     4741 gaggcccacc ggcaaaggcc aacaagtgaa aggaaacacc ctgctcaata ttttccatca
     4801 catactgcct ggtataaccg atggtgagca aggcggaaag tccatcggca gg
//
LOCUS       DAOQNZ010000096         4840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7418_length_4840_cov_3.619854, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000096 DAOQNZ010000000
VERSION     DAOQNZ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7418_length_4840_cov_3.619854"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PKW73_02645"
     CDS             complement(<1..706)
                     /locus_tag="PKW73_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNA72209.1"
                     /translation="MRAVTITTSGNPDVLQIAERPEVKPETWEVLVKVEAFGINRADV
                     LQRKGLYPPPPGVPADIPGLEFAGIVEAVGKAVSLFRPGDRVFGLVGGGSYQERLCQH
                     ELALLPIPQNLSIEEAAAIPEAFITAFDALVLQAGLKHSDRVLISAVGSGVGMAAAQL
                     ALAFQCRVAGSSRSSEKLERAASFLGQPQSQFLPVLVEDGTFAKKISTAFGAIDVFLE
                     LVGGAYVEEDLKCAASK"
     gene            893..1696
                     /locus_tag="PKW73_02650"
     CDS             893..1696
                     /locus_tag="PKW73_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72210.1"
                     /translation="MAKENNSSISETEAVTAAPEPAAKVAKDNWDKLAAVAPIVSGAL
                     IFLMGGYFTYSYNQQQLRLQEIQTIERFIPHLMGNNQSKRAAILAISSLTNAELASKF
                     AQIFASQGTVSALQSIAETGSDKEKTVATTALAQALENLAARESRLNEMESAFQKALG
                     DKQTRREHGSRAEAEQLGLDEPDEASKLEKLAQMCRERGQTSVAESLLRQAQLIRGRH
                     ENRDKEEKPPAPPLPAPQAEKAPEEPKSQDKLQSVPDSEKTTSELPVAQ"
     gene            complement(1713..1943)
                     /locus_tag="PKW73_02655"
     CDS             complement(1713..1943)
                     /locus_tag="PKW73_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72211.1"
                     /translation="MSLSKIKEAISAGKMLCPTCQKPVKSFEKYVELTASVWDGAGDS
                     NLETAGSKVTLICGNDDCSWKERTEYWAEYIK"
     gene            2117..2533
                     /locus_tag="PKW73_02660"
     CDS             2117..2533
                     /locus_tag="PKW73_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNA72212.1"
                     /translation="MSYEIEQLLLKHGVKPTPQRAVIAQFLLSTHSHPTADEVFQAVS
                     ESLPVPLSRATVYNTLNCLVEAGVLIEVVVEPGKSRYDANTSQHHHFVDTESGKIIDV
                     RPEEVKNLPLKLSEGFLDGAFDVEDYRITFFGKRKG"
     gene            2609..3085
                     /locus_tag="PKW73_02665"
     CDS             2609..3085
                     /locus_tag="PKW73_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNA72213.1"
                     /translation="MNIEASNTKKNLEAAFAGESMANRKYLFFAKMARQLGDEEVAAL
                     FEKTANEETAHAFAHLELIYPSQELTVERLLELAIEGELYETNVMYPQFEQEAIAEGK
                     AEAALEFVEQANESRVHAEIFASAAKRFKALKIVEAFHAGRYQKMLDKKTPQKKAG"
     gene            3169..3594
                     /locus_tag="PKW73_02670"
     CDS             3169..3594
                     /locus_tag="PKW73_02670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNA72214.1"
                     /translation="MTQKVSARCFEVGLDVRVSTYDIDYAGHVSNISYFRWLEDMRLK
                     ILEEYFPLEGLMADGYLPILAGSQIKYKKAIRLFDKPRGRMWIDGITAATMTFRGEFT
                     VDGEVYTEASHQGLFISAQTHKPVRLPPQIVKMYKQFEK"
     gene            3613..4407
                     /locus_tag="PKW73_02675"
     CDS             3613..4407
                     /locus_tag="PKW73_02675"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF009093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HNA72215.1"
                     /translation="MTAFKTSARSAVVTGATSGIGRAVALALAEKHLNIAINGFGSEA
                     EIEKSLTDLKAAGAKEAIHVPTDMSRVTEVEHFINEALKAFGEVDVLVNNAGIQHTAL
                     VEEFPVEKWEQVISINLSASFHSIRMCLPSMKAKNWGRIINIASVHGLVASTQKAAYV
                     AAKHGLVGLTKVVALECADTGITCNAVCPGWVETPLVQKQVEAIAAVDGISIAEARHR
                     LISEKQPSNRFVALDELAQFCLYLVSDLAGSITGSSLPIDGGWTAR"
     gene            4410..>4840
                     /locus_tag="PKW73_02680"
     CDS             4410..>4840
                     /locus_tag="PKW73_02680"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNA72216.1"
                     /translation="MSRAVLLSLVVALSCSILGLEARPGGTVQKKKTAASRSPVAGTA
                     TKSVQASPDSALEKNVRAWLERPEIRHSRVGVEVMDLASGQVLFQSNGNKRHTPASTA
                     KILTTACLYDLVGKDFVYKTRLLTNGSVKEDLLNGDLAIET"
BASE COUNT         1225 a         1202 c         1294 g         1119 t
ORIGIN      
        1 ccttactggc ggcacatttt agatcttctt ctacgtaggc accgcccact aattcaagaa
       61 agacatctat ggcaccaaag gcggtgctga tcttcttggc gaaggtaccg tcttccacca
      121 gaaccggcag gaactgtgac tgcggctgcc ccagaaaaga cgcggcacgc tctagctttt
      181 cgctggaacg actgctgccg gccactcggc actgaaatgc cagggccagc tgggcagcgg
      241 ccattcccac gccactacct acggctgaga tcaagacccg gtctgagtgt ttgagcccgg
      301 cctgcaaaac aagggcatca aaggcggtga taaatgcttc aggtatagcc gcggcttcct
      361 ctatagagag attttgcggt atgggaagca gagccagttc gtgctgacag agccgctcct
      421 ggtagctgcc gccgccgaca aggccaaata cccgatcgcc tggacgaaag aggctgacgg
      481 ctttgcctac agcttccacg atgccggcga attccagacc tggtatgtcg gctggtacgc
      541 ccggcggcgg cggatagaga cctttgcgtt gcaagacatc ggcgcgattt ataccgaatg
      601 cttcgacttt tacaagaacc tcccaggtct ccggttttac ctcgggtctt tctgcaatct
      661 gcaatacatc cgggtttcca ctagtggtga tggtgacggc gcgcaagatt cttgtcctta
      721 attatctatt tgccgggatt ttacaaagca agatttacgt aacgaactgg gcagcgggcg
      781 gtaaagtgac tcgacaggag cagtttacaa cctgtatcct ctgagggtac ttatccgtac
      841 caaattttag cgttttaagc gtggcgaaac accagcatgg ctaaagatca acatggctaa
      901 agaaaataat agttcgatat ctgaaacaga ggcagtgacc gcagcgccgg agcctgcagc
      961 gaaggtggcc aaagacaact gggataaatt ggcggcagta gcaccaattg tttccggtgc
     1021 tttgatcttc ctcatgggtg gatattttac ttattcctat aatcagcaac agctcaggct
     1081 gcaggaaatt cagactatcg agagatttat tcctcacctg atgggcaaca accagagcaa
     1141 gcgagcggct attctagcca tttcttcttt gactaatgct gaattggcat ccaagttcgc
     1201 tcagattttt gccagccagg gaacggtttc agccctgcaa tcgattgccg agaccggctc
     1261 tgacaaagag aagactgtag ctacaacggc tctagctcag gctcttgaaa atcttgccgc
     1321 cagagaaagt cggctcaatg agatggaatc ggcattccaa aaggcgctgg gtgataagca
     1381 gacaaggcgc gaacacggtt ccagggcgga agctgagcaa ctgggactgg atgaaccgga
     1441 tgaggccagc aaactggaaa agctggctca gatgtgccgg gaacggggac aaaccagtgt
     1501 ggcagagagc ctgctcaggc aggcccagct tattcgcggg cgtcatgaga acagagataa
     1561 ggaagagaag ccgccggctc cgcctctacc agctccgcag gcggagaaag cgccggaaga
     1621 gccgaaaagc caggataagc tccagtctgt gccggacagc gaaaagacta cttccgaact
     1681 gcctgttgcc cagtagcgct tactttgtat atctatttta tatattccgc ccagtattca
     1741 gtacgttcct tccagctaca gtcatcattg ccgcaaatga gggtgacttt gctgccggcc
     1801 gtctccaggt tggagtcacc ggcgccgtcc cataccgagg cagtcagttc cacgtacttt
     1861 tcaaagcttt tgacgggctt ctggcaggtg gggcagagca ttttgcctgc agagatggct
     1921 tctttgattt tggaaaggga cataaattgc tagccttact attaagtaga acgaagttgg
     1981 tttgccggcc ctaaagccta gcaggttata gattttaccc tccctaccgg cagccaaatg
     2041 aaagatctgt agaaaagtgg ttgacaaagt ctacagttag aattattcta agtagtgtgg
     2101 ttaggaaatg agagcaatgt cttacgagat cgaacaatta cttctcaaac atggggtgaa
     2161 gccaacgccc cagcgcgctg taattgccca gtttctgctt tctacccaca gccatccgac
     2221 ggccgacgaa gtattccagg ctgtatcgga gagcttaccc gtaccccttt cccgggccac
     2281 ggtctacaac accctcaatt gcctggtgga agccggggtt ctcatcgagg tggtggtaga
     2341 accgggcaag tccaggtacg atgcaaacac cagtcagcat catcattttg tcgatacaga
     2401 aagcggcaaa atcattgatg tcagaccgga agaagtgaaa aatttgccgc ttaagctttc
     2461 ggaagggttt cttgatggtg ctttcgacgt ggaagactac cgcataacct ttttcggcaa
     2521 gcgtaaaggg tgattttttt tgcctagcac ttagactgtg tctaaaagta gaagaactct
     2581 atatagttga attatgagga tataaatcat gaatatcgaa gcttcgaata ccaaaaaaaa
     2641 cctggaagct gcctttgccg gtgagtctat ggcaaaccgc aagtacctgt tcttcgccaa
     2701 aatggcaaga caactggggg acgaagaggt ggccgctctc tttgagaaaa ccgctaatga
     2761 agaaacagcc cacgctttcg ctcatcttga gcttatctac cccagccagg aactgactgt
     2821 ggaaagactt ttggaactgg ccatagaggg cgagctctat gagacgaatg tgatgtatcc
     2881 ccagttcgag caagaagcca tcgcggaagg caaggccgag gctgcccttg agtttgtgga
     2941 gcaggccaac gaatcacgtg tccacgctga gatcttcgcc agcgctgcca agcgtttcaa
     3001 agctctcaaa attgtggaag ctttccacgc cggtcgctat cagaaaatgc ttgacaaaaa
     3061 gacgccgcaa aagaaagccg gttaagtcat acatcatact ttacaagcgg aaggagctac
     3121 cccgggtgag gctgatatga tcatggcgtc ctcaccccgg agtgtccctt gactcaaaaa
     3181 gtctctgctc gttgttttga agtcggcctt gatgtgcgtg tttcaactta cgacattgac
     3241 tatgccggcc atgtgtcaaa catcagttat tttcgctggc ttgaagacat gcgcttgaag
     3301 attctggaag aatatttccc cctcgagggt ctgatggcgg acggctacct gcctatcctg
     3361 gcgggcagtc agataaagta caaaaaggcc atcaggctat ttgataagcc ccgtggcagg
     3421 atgtggatcg atggtatcac tgctgccacc atgacctttc gcggagagtt cacggttgat
     3481 ggagaggtct ataccgaggc ctcccatcag ggtttgttca tttcggctca aactcataaa
     3541 ccggtgagat tgccgccgca gattgtaaaa atgtataagc agtttgagaa ataaaggagt
     3601 taggcagcct caatgacagc atttaagact tcagccagat cggcggtggt gactggcgct
     3661 accagtggta tcggcagggc ggtggccctg gccctggctg agaagcattt gaatattgcc
     3721 atcaatggtt ttggttcgga ggctgaaata gagaagtcgc tcaccgacct gaaggccgcc
     3781 ggagcaaaag aagcaatcca tgtgccgacg gatatgtccc gcgtcacgga agtcgagcat
     3841 tttatcaatg aagcactcaa ggccttcggc gaggtcgatg tgctcgtcaa taatgccggc
     3901 atccagcaca cggctctggt ggaggagttc cccgtagaga agtgggaaca agtgatttcc
     3961 atcaatctct ccgcctcttt tcatagcatc cgtatgtgcc ttccttccat gaaggctaag
     4021 aattggggac ggatcatcaa tatcgcttcc gtgcacggac tggtggcatc cacccagaaa
     4081 gcagcctatg ttgcggctaa acatggttta gtcgggctga ccaaagtggt ggccctggaa
     4141 tgtgccgata ccggtatcac ctgcaatgcc gtttgtccgg gctgggtgga gaccccactt
     4201 gtccagaagc aagtggaggc tattgccgcc gtagatggaa tttccatagc cgaagccaga
     4261 catagattga tcagcgagaa acagccctct aaccgtttcg ttgccctgga tgaactggca
     4321 cagttttgtc tctatctggt atcagatctg gccggttcca tcaccggctc cagtttgccg
     4381 atagatggcg gctggacggc aagatagaaa tgtcgagagc cgttttgcta tctctggttg
     4441 tggcgctctc ctgcagtatt ttagggcttg aggccaggcc tgggggcacc gttcaaaaga
     4501 agaaaacagc ggcttccaga agtcccgtgg ccggcactgc taccaaatcg gtgcaggcta
     4561 gtcctgactc ggccctggaa aagaatgttc gagcctggct ggaaaggcct gagatacgcc
     4621 actcccgcgt gggcgtggaa gttatggatc tggccagcgg tcaggtattg ttccaatcaa
     4681 acggcaataa aagacataca cctgcttcca cggcgaaaat tctcactacg gcttgcttat
     4741 acgatttagt cggcaaagac tttgtctaca agaccagact cttaaccaat ggcagtgtca
     4801 aggaagacct tttgaatggt gacctggcta ttgagaccag
//
LOCUS       DAOQNZ010000097         4830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7450_length_4830_cov_3.575707, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000097 DAOQNZ010000000
VERSION     DAOQNZ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7450_length_4830_cov_3.575707"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /gene="mutM"
                     /locus_tag="PKW73_02685"
     CDS             <1..748
                     /gene="mutM"
                     /locus_tag="PKW73_02685"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNA72217.1"
                     /translation="DSIAFPDPDQFRRLLPGHGFLSVRRRGKYILIDLDEDAALVCHL
                     RMSGRLLIRARGAGANDARFLRVKMPLASGRQLHFEDMRVFGRLWYKPRGVSLEEVVP
                     ALSELGLEPLTDLTAAKLKLLFAGKKQAVKTTLLDQTLIAGVGNIYADEALFLTGVHP
                     QTSAGDLTARSLTRLVESIQLVLSRGIESGGSTLRDYVNAEGVNGNYQNQAWVYGRED
                     EPCLKCGREIVRVKVAGRSSHFCPGCQKRR"
     gene            complement(745..1581)
                     /locus_tag="PKW73_02690"
     CDS             complement(745..1581)
                     /locus_tag="PKW73_02690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA72218.1"
                     /translation="MPKARSKDKTASLLPESLALTPEQFQARSTSNFAAQYLPAGARV
                     LDVGVGSGMIARNLMDGGFKLEAVDIDPARVKEAEAIGIKARVCDFKDLKGEPYDAIL
                     FSRVLHHLQPLRTNIDTTIKMLKPSGIVIVEDFSYERVDSRTCAWLFPLAKMLKDRQE
                     PKAGQASRHSWLCEQAVSQASSPEEYVRQALHSWRHHHEEKHNITPFEHIKSVMQDNF
                     RFLLDARVPYLFRYLADLLPHTIDGGKLACELAHWEAALAESGAIAPVGVRLVAKPKK
                     KN"
     gene            complement(1596..2657)
                     /locus_tag="PKW73_02695"
     CDS             complement(1596..2657)
                     /locus_tag="PKW73_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNA72219.1"
                     /translation="MEVKQESDETAGSNQEKSVKKSNNLVKQIVSGLFAVVLLYLAFH
                     NVDPQKLWQYSRDIDPLPLVPVCLTAIFSHVLRAYRWTFLLYPVAGRKIGLFNSFYAV
                     ILGYAVNVAIPRGGEVARLLSICKDEKLPWAGVLPTMLIDRMLDMAMLVFLLGVCLLV
                     LPKSLLAAVPGLIPGGIGLLIATIIGLLLLPNVGAILKAILNMDLCRKKLPAALHEKL
                     LELAAQFESGTRALKSPVTYPLIAALSFVIWSMYWLNFYFMLPAFHLDKLVGPMSCLI
                     VFTIGSVGVLIPTPGNAGSFHFLVALALTKVAPTIPYEQALAYATVLHVLTFVMTNCI
                     PAALLFLYRKTRESKPSST"
     gene            2971..4662
                     /locus_tag="PKW73_02700"
     CDS             2971..4662
                     /locus_tag="PKW73_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HNA72220.1"
                     /translation="MEARKTKVLLIEDGKEVSHDLKDVLKTMAHEFTMEVTDHLAGGL
                     KVLSEGNFDVVVLDVSCSDQVSDGLSHFLKLREKDASVPVVMLGSREQDKVGSEAVKQ
                     GVKDFLVKDELDGRVLLQSIRYASTLKRMEDELTALKAKLVQTEKQLDMIANVDASTQ
                     ALSRAGLERALEAEYVRAQRGGWNLVAMLIDCDDFDRINRQFGHAVGDVVLRQITERI
                     RETVRPTDHVARMGGDEFLLILPDTRIAEGMLVAEKIRLAVGESPLRLASETVRVTAS
                     LGVLALPYEFCSIEEVLSLARLAVRESKLLGKNRVSSGEKNPSLTPSLSPDKAALDEL
                     TEKLRKGDCFRAVSMPIFHLSDEKVVGYEILSRGPAGAFEMPDDLFRVSVEHNLLTIV
                     DLRCLKTCLSACSDPKFDQKARFHVNLFPSTIMDTPIDRLMTLFPQETHEGTFCVEIS
                     EQQFIGDPAYLKDHVHAIRKRDILVAIDDVGFGRSSLESLIILEPDIVKIDRKYVSGI
                     SADPAKARLLKRLVKVVNSIGAELIAEGIECREELELLVEMGVPFGQGWLWGKPA"
BASE COUNT         1081 a         1220 c         1400 g         1129 t
ORIGIN      
        1 agactccatc gccttccccg accctgatca gttccgtcgc ctgctgcccg ggcatggctt
       61 tctgtctgtg agaaggcgag gcaagtacat tctcattgat cttgacgaag acgccgctct
      121 ggtttgtcac ttgcgtatgt cagggcggct tctgattcgc gccagagggg ccggcgccaa
      181 cgacgcgcgt ttcttgcggg tgaagatgcc gctcgccagc gggcgccaac tgcattttga
      241 agacatgcgt gtcttcgggc gcctctggta caaaccccga ggggtgagtc tggaagaggt
      301 cgtaccggct ctttcggaac tggggcttga gcctctaacc gatttgacgg ccgctaaact
      361 aaagttgctc tttgccggca agaagcaggc ggtgaagacg accctcctgg accagaccct
      421 gattgccgga gtgggtaata tctacgccga tgaggctctc ttcctgaccg gtgtgcatcc
      481 ccagaccagt gccggcgatc ttaccgccag gagcctgact cgactggtcg agagcattca
      541 actggtcttg agccgtggta tcgaatctgg tggctcaact ctgcgcgatt acgtgaacgc
      601 cgaaggcgtc aacggtaatt atcaaaatca ggcctgggtg tacgggcgag aagacgagcc
      661 ctgcctcaag tgcggacggg aaattgtcag ggtgaaggtg gccgggcgtt cttcacactt
      721 ttgccccggc tgccagaagc gccgctaatt tttcttcttg ggcttggcta ccagtcttac
      781 tcccaccggt gcgatggcgc cgctttcagc cagagctgct tcccagtggg ccagctcgca
      841 ggccagtttg ccaccgtcta tggtgtgagg taaaagatct gccagatagc gaaaaagata
      901 gggcactcgc gcgtccaaaa ggaagcggaa gttatcttgc atgacgctct tgatatgctc
      961 gaagggcgta atgttgtgct tctcttcgtg gtgatggcgc cagctatgca aagcctgcct
     1021 gacgtattct tccggagagg atgcctgact gacggcttgc tcgcaaagcc aggagtgccg
     1081 gcttgcctgc ccagctttgg gttcctgccg gtctttcagc atcttggcca gcggaaatag
     1141 ccaggcgcag gtacggctgt ctacccgttc gtaggagaaa tcttccacaa ttacgatacc
     1201 gctgggctta agcatcttga ttgtggtgtc gatattggtg cgcaagggct gcaggtgatg
     1261 cagcacccgg ctgaagagaa tggcatcata gggttcaccc ttgaggtcct tgaaatcgca
     1321 aaccctggct ttgatgccga tggcctccgc ttccttgacc cgggccggat ctatatccac
     1381 agcctccagt ttaaatcccc catccataag atttctggcg atcatgccgg aaccgacccc
     1441 cacgtccagc accctggccc cggcaggcag gtactgcgcg gcaaaattac tggtggaacg
     1501 agcttgaaac tgctccggtg taagtgcgag gctttccggt aatagggaag cggttttgtc
     1561 tttcgatctg gctttgggca ttagtgctgc ttctctcagg ttgatgaagg tttgctctct
     1621 ctggtctttc tgtacaggaa gagcaaagcg gcgggaatac agtttgtcat gacaaaggtg
     1681 agcacgtgta agactgtggc ataggccagg gcttgttcat agggaatggt gggtgctact
     1741 ttggtgaggg ccagtgccac tagaaagtga aagctgccgg cattgccggg tgtgggaatc
     1801 aagactccca ccgaaccaat tgtgaagaca atcaggcagg acatggggcc cactaactta
     1861 tccaggtgga aggccggcaa cataaagtag aagttgagcc agtacatgga ccagattacg
     1921 aagctgagag cggcgatgag gggataggta accggacttt tcagggcccg ggtaccgctt
     1981 tcgaattgtg cagccaactc caggagcttc tcatgcaggg cggccggcaa tttcttgcga
     2041 cagagatcca tattgaggat agccttgaga atggctccga cattgggcaa gagtaagagt
     2101 ccgataattg tggcaatgag cagaccgata ccgcccggta ttaagcccgg taccgccgcc
     2161 aaaaggcttt tgggcaagac cagcaggcag acgccaagca gaaagaccag catggccata
     2221 tccagcatgc ggtctataag cattgtgggc agtacgccag cccagggcaa tttttcgtct
     2281 ttgcaaatgg aaagaaggcg ggccacttct ccgcctcttg gtatggcaac gttgacggcg
     2341 tagcctagaa tgacggcata gaaagaatta aaaagtccga tcttacgacc ggccaccggg
     2401 tagagcagaa atgtccagcg gtaggctctc agcacatggc tgaaaatggc ggtaaggcag
     2461 actggcacaa gcggcagcgg gtctatatcc cgtgaatatt gccagagttt ctggggatcc
     2521 acattatgga aggccaggta aagcaggact actgcaaaaa gcccggaaac gatttgtttg
     2581 acaaggttat tggacttctt gaccgacttc tcttggttac tgccggcagt ttcatcactt
     2641 tcctgtttta cttccaagtt tggttagcct taagtcgggg aactaaccaa taaggttact
     2701 atgggtacac tctacataga agaactatgt gcaagtttgc cgagttcaag ttgtaatttg
     2761 agctacgttt ggggtttgac tgggcacaat agatttgtaa gcgtcaggct tgaaaggaag
     2821 agtgcataga ttttgtcaag ggtcctgtga gactttgtac tgttcggtga tttcttgcta
     2881 ggtaggcggt ttacaatatt gatatgccgg gttgcaagtt agcttgtaaa ctcttgggca
     2941 atcaaatgga aacctttgtt cggtggtgaa atggaagcca ggaaaactaa agtactcttg
     3001 atcgaggatg gcaaagaggt tagtcatgac ctcaaggatg tcctcaagac aatggcccat
     3061 gagttcacca tggaggtcac cgaccatctg gctggtgggc tgaaggtgct ctccgagggc
     3121 aattttgatg tggtcgttct ggacgtgtcc tgcagcgatc aagtcagcga tggactttcg
     3181 catttcctta aattgagaga gaaagatgcc agcgtgccgg tggtgatgct gggctctcgc
     3241 gaacaagaca aagtcggtag tgaagccgtc aagcagggtg tcaaagattt tctggtgaaa
     3301 gacgaactgg atgggcgtgt gctcttgcag tccattcgtt atgcttccac cctcaaacgc
     3361 atggaagacg agctgactgc tttgaaagcg aagcttgtgc aaaccgaaaa acaactggat
     3421 atgatcgcca acgtggatgc ttccactcag gccctcagcc gtgccggtct ggagcgagcc
     3481 ctggaagctg agtatgtgcg cgcccaacgc ggcggctgga atctagtggc catgcttatc
     3541 gactgcgacg acttcgatcg catcaatcgc cagttcggcc acgccgtggg cgatgtggtg
     3601 ctccgtcaaa taaccgaacg catccgggag acggtgcgtc ccaccgacca tgtggctcgt
     3661 atgggcggcg acgaattcct attgattttg ccggacaccc gcatcgcaga aggtatgctg
     3721 gtcgctgaga aaatccgcct ggccgtgggt gagagcccct tgcgtctggc ttccgagacg
     3781 gtgcgagtga cggccagctt gggtgtgctc gccctgccct atgaattttg ttccattgag
     3841 gaagtgcttt cgctggcccg gctggctgtg cgcgaatcga agttgctcgg taagaaccgc
     3901 gtctccagcg gcgagaagaa tccgagcctg acgccgtctc ttagccccga caaggctgct
     3961 ttagacgaat tgaccgagaa gctgcgcaaa ggcgattgct tccgggctgt ctccatgcct
     4021 attttccacc tctccgatga gaaggtcgtg ggctatgaaa tcctcagccg tggtccggcc
     4081 ggtgcttttg agatgcccga tgatcttttc agagtgtcgg tagagcacaa cttgctcacc
     4141 atagtcgact tgcgttgtct caagacctgt ctttcggcct gcagcgatcc taagtttgac
     4201 cagaaagcgc gctttcacgt caatctcttc ccctccacca tcatggacac tcccatcgac
     4261 aggctgatga cgcttttccc gcaggagacc cacgaaggta ctttctgcgt agaaataagc
     4321 gaacagcagt tcatcggtga tcccgcttat ttgaaagatc acgtgcacgc tatcagaaag
     4381 cgggacatcc tggtggctat cgatgatgtc ggcttcgggc gcagctccct ggagagcctc
     4441 attatcctcg agccggatat agttaaaatc gaccgcaagt acgtctccgg tatctccgcc
     4501 gatccggcca aagcaagact gcttaagcgg ctggtgaaag tagtcaactc tatcggcgcc
     4561 gaacttattg ccgaaggcat cgagtgtcgg gaagaacttg agctactggt ggaaatgggt
     4621 gttcctttcg gtcagggctg gctctggggt aaaccggctt aaggctgagt atttccgtac
     4681 cggcaacgtt gtgttctcct taccctgaga tcagttgtcg atgcagcagg accataatca
     4741 aggccaggca aacggagagc tggatccctc ctgcagtctc ggcttgggtg aaataatcga
     4801 cggaaaatat acggtgctca gcttgctggg
//
LOCUS       DAOQNZ010000098         4773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7576_length_4773_cov_4.143705, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000098 DAOQNZ010000000
VERSION     DAOQNZ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7576_length_4773_cov_4.143705"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PKW73_02705"
     CDS             <1..1671
                     /locus_tag="PKW73_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72221.1"
                     /translation="HGSSNDPLQGALKSLETTSLPLQSEAREASEAKPAAAPTPIQGA
                     PVSIGIDSGTRAEIERQLADHLAGRPLQSPQVGDLERGTQEIAIDVPVALNDEEQNCV
                     KTYPGSAELEPRQEPQEGRTLGLPGECRDRHETSTFPGRIEEPNSAMEAVNVSPERES
                     FPLKLEQPTELSPVEKSSLAPDPPREVEAAVPGPERAMDTAKPPLVKDKGDHRTVRSE
                     VRRAEPKYFPLSVNVEKLTETVSFQRLFSGIPLVLTGSLRPTAQVAYLLVPLPPAPQA
                     ELSEPSTAESPASAPPQVKGRWQQYCFVPLLTLPGKQTIAENRQGELVPERGGEFFTA
                     RPLMPVTGPQTAERVPLPESEKGEKLVSPAQPGVHPGAEPGLSASLSLQPGVTAAGPE
                     LPVKSRDRYYISGPDLVFAAIIALASTASRRHDDLTEAVESGSPSASVLVNVLCERSR
                     PSLIVSHGDNLEAIAERFFNDRRLSHLIADINERVICQCQLDGKRIVRIKVGQVLQLP
                     LLEDITDFHSRRANKEKCRQRLLTIVEDTEVSKEVLMETFKPFLKRLS"
     gene            complement(1677..2447)
                     /locus_tag="PKW73_02710"
     CDS             complement(1677..2447)
                     /locus_tag="PKW73_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA72222.1"
                     /translation="MKTVKVLICEDLPSLREHARQVVSQTLERQEITPQFEEAANGAA
                     AVRLAASFHPDLILMDIAMPELNGIKAASQIWKQTPTMKILFWSQYQHEAYIRELGRI
                     VPDEAIHGYLLKSEDDRSLSEALVSVLLLDLPFIGAQIEAVHKRLKDKSSAITDAEYE
                     TLLDVFAGLTDKAIAAKEHISYRGAQNRLSNLLIKLLKNEDAYLKESAGKEIFNPRTR
                     LVFEALRRGLLNLEDLENQRKHLDDWLFTEFGFSREEG"
     gene            complement(2444..3550)
                     /locus_tag="PKW73_02715"
     CDS             complement(2444..3550)
                     /locus_tag="PKW73_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA72223.1"
                     /translation="MSAELNDRILPRFFLLLTASAALIASCLTPLDSGLSEFLGLPVC
                     SDLTALLLRTALACATMAGLLMTALKVKAANRPIVYLQISALSLILQWWLNKSLQMVP
                     MPSTLVVLFLLAAQSGGAYRQLTLRLEKAAADHYGLLLKEQEVLAAKLALLRQEEADR
                     RLLASDLHDQVLQDLKQLKAALNGLSTKLDAADLAPIETLIDSSTTQVRAVMENLFPS
                     VLENLGFKSALSELLRQNTLKGGFRAHLTCPEEVPELSPIEKLLLFRIVQEVLNNIVK
                     HAQAGQVTLQVEAAPASLKITIKDDGKGLPSDFSALTKQAEERGSRGLRYIEQRADLL
                     QAAVDWQSAGSGTTFTLILPLVRQQNHQTEEVST"
     gene            complement(3571..4479)
                     /locus_tag="PKW73_02720"
     CDS             complement(3571..4479)
                     /locus_tag="PKW73_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72224.1"
                     /translation="MKPNRIRKTAHVKIIAVGLLTQMLTGFTACQAASPEFRRHYELG
                     EDFLERDQYKLAIPEFDEAIKAEGGKGKDAAAALVQRGTAYSALASYDRALKDLSQAV
                     KMDPASDLGYNNRGAVYLRTQKPEAALADFDRAMQINPKNKYAAVNRAGVFLLMTDPG
                     KRAAATLRWLDAGGWQTDFAGHAAILSSLAYLASGDRKNFDALVALSLKKLDRLKWPY
                     SVLKHFQGKADAEAVLEQAQDSTYNLTQAQCFLALEDYFKGKKDGISARLDFVDKHGT
                     INSVEYWLGKAFLTKLRSPGKPLANK"
BASE COUNT         1043 a         1239 c         1358 g         1133 t
ORIGIN      
        1 cacggcagct ctaatgatcc gctccagggt gctttgaaga gcctggaaac gacttcgctt
       61 cccctccagt cggaagccag ggaagcaagc gaggccaaac cggcagctgc tcccacgccg
      121 attcagggag cgccggtctc catcggcatc gactccggca cgagagcgga aattgaaaga
      181 cagctcgccg accatttagc cggacgacct cttcaatcgc cgcaggttgg ggatttggag
      241 cgtggtacgc aagagattgc catagatgtc cccgttgctc tcaatgacga agagcaaaat
      301 tgtgtgaaaa cttacccggg ttcggcggaa ttggagcccc gtcaagagcc gcaagaggga
      361 cggaccttgg gcctgcccgg ggagtgccgg gaccgtcatg agacttccac ttttcccggt
      421 cggattgagg agccaaatag tgcgatggaa gccgtcaatg tctcaccgga gagagagtct
      481 tttcctctta aattggagca gccgaccgaa ctttccccgg tcgagaagag ttcccttgcc
      541 cccgacccgc cgcgagaagt agaggcagct gtgcctgggc cggaaagagc aatggacact
      601 gctaagccgc cacttgtgaa agataaaggc gaccatagaa ccgtgaggtc tgaggtccgc
      661 cgggctgagc cgaagtattt ccccctctca gtaaatgtgg aaaaacttac tgaaacggtt
      721 tccttccagc gtcttttcag tggcattcct ctagttttga caggctctct cagacccact
      781 gcgcaggttg cgtacctgct tgtgccactg ccgccggcac cacaagcgga gttgtccgag
      841 ccttcgacgg cggaatctcc ggcttccgcg ccgccacagg ttaagggccg gtggcagcag
      901 tattgtttcg tgccactgct taccttgcct ggcaagcaga caattgcgga aaacaggcaa
      961 ggggaacttg tgccggagag gggcggcgag ttcttcaccg ccagaccgct gatgccggtg
     1021 accgggcctc agaccgcaga gcgagtacct ttgcctgaaa gtgaaaaagg ggagaaactc
     1081 gtttctcccg ctcagcccgg ggttcatcca ggcgcagagc cagggctctc ggcatcctta
     1141 tccctgcagc ctggcgttac cgctgccgga ccggagcttc ctgtaaagag ccgggaccgg
     1201 tactatataa gcggaccgga tctggttttt gcggcgatca ttgccctggc cagcacggcc
     1261 tcccgtcgcc atgacgatct cactgaggct gtggagagcg gctccccgtc cgctagcgtt
     1321 ctggtaaacg tgctttgcga gcgctctcgc cccagcctga tcgtcagtca tggtgataac
     1381 ctcgaggcta tagccgagcg ttttttcaac gaccgacgcc tgagtcactt gattgccgac
     1441 ataaatgaaa gagttatctg tcagtgccaa ctggacggca aacgtattgt ccggatcaag
     1501 gtcggacaag tgctccaatt gccgcttctg gaagatatca ccgactttca cagccgacga
     1561 gcgaataaag aaaagtgccg ccagagattg cttacgatcg tggaggatac ggaagtgtca
     1621 aaagaagtcc tgatggagac ttttaagccc tttctgaaaa ggctgagctg actctctcag
     1681 ccttcttccc tgctgaaacc gaattcggtg aacaaccagt cgtccagatg ctttctctga
     1741 ttctccagat cttccaggtt gagcagacct cgtctcagcg cttcaaaaac caatctggtg
     1801 cggggattga aaatttcttt gccggcgctt tctttcaggt aggcatcctc gtttttcaag
     1861 agtttgatca gcaggttgga aagacggttc tgagcgccgc gataagaaat atgctccttg
     1921 gcggcgatgg ccttgtccgt aagaccggcg aaaacatcca gaagagtctc atactcggcg
     1981 tcggtaatgg cggagctctt gtctttgaga cgcttatgga cggcttctat ctgggcaccg
     2041 atgaaaggca gatcgagtag aagtacgctc actaatgctt cactgaggct gcggtcgtct
     2101 tcagacttca atagatagcc gtgaattgcc tcatcaggca ctattctgcc cagttctctg
     2161 atataagcct cgtgttgata ttgggaccag aagagaatct tcatggtcgg agtttgcttc
     2221 caaatctgac tggccgcctt gattccattc aattccggca tggctatatc catgaggatc
     2281 aaatccggat ggaaggaggc tgccagccta actgcggcgg ctccgtttgc ggcctcctcg
     2341 aattggggag tgatttcctg ccgctccaat gtttgagaaa cgacctggcg agcgtgctct
     2401 cttagagaag gcaggtcctc gcaaattagt actttcactg ttttcacgtt gagacctctt
     2461 cggtttgatg gttttgctgc cgcaccaggg gtaatatcag ggtaaaagta gtgccgctgc
     2521 cggccgattg ccagtcaaca gcggcctgca aaagatctgc tctctgttcg atgtagcgta
     2581 agccacggga gcctctttcc tcagcctgtt ttgtcagtgc ggaaaagtcg gacggtaggc
     2641 ctttgccgtc gtctttgatt gttattttaa ggcttgccgg cgctgcctcc acctgcaagg
     2701 taacctggcc ggcctgcgca tgtttgacaa tattgttgag gacttcctgc acaatgcgaa
     2761 agagcaatag tttttcgatc ggactcagtt ccggtacttc ctcaggacag gttaaatggg
     2821 cgcgaaaacc gcctttgaga gtgttctgcc tcagtagttc ggagagtgca cttttaaaac
     2881 caagattttc cagtactgat ggaaagaggt tttccataac cgccctgacc tgggtggtgc
     2941 tcgaatcaat cagggtttct atgggggcca ggtcggcggc atcaagtttt gtagaaagcc
     3001 cattcaaggc tgctttgagc tgcttgaggt cctgcagcac ttgatcgtgc aggtctgatg
     3061 ccagaagccg cctgtccgcc tcttcctgtc tgagcaaggc cagtttggcg gcgagcacct
     3121 cctgttcttt tagaagcaaa ccatagtgat cggccgctgc tttctccagg cgcagggtca
     3181 actgccgata ggctcctccg ctctgggcgg caagcaagaa gagcactacc agagtcgagg
     3241 gcatcggcac catctgcagt gatttgttca gccaccattg caggattaag ctgagagctg
     3301 agatttgcag gtagacgatg gggcgattag ctgcctttac cttcaaggcc gtcattagta
     3361 aaccagccat ggtggcacag gccagagcgg ttcgcaagag aagcgccgtc aaatctgagc
     3421 aaaccggcag cccgagaaac tcgctcaggc cactgtcgag aggggtgagg caactggcaa
     3481 tcaatgctgc tgaagctgtg agcaggagga aaaatcttgg caggatccga tcgtttagtt
     3541 cggcagacat ttttgttccg aggagtgtgg ctatttgttt gcgagcggtt tgccgggact
     3601 tcttagttta gtgaggaagg ctttgcccag ccaatattcc accgaattga tggtgccgtg
     3661 cttgtcgaca aaatcaagtc gtgctgaaat gccgtctttt ttgcctttga aatagtcttc
     3721 cagggccagg aagcactggg cctgggtgag attataggtg ctgtcctggg cctgctccag
     3781 cactgcttcg gcgtcggctt tgccctggaa atgcttgagt acggaataag gccacttgag
     3841 gcgatcaagc ttcttgaggg aaagagcaac cagggcgtcg aagttttttc gatcgccgga
     3901 agccaggtag gccagagagg aaaggatagc cgcgtgtccg gcgaaatcgg tttgccagcc
     3961 cccggcatcc agccagcgca aggtagccgc tgccctttta ccagggtcgg tcatgagcaa
     4021 gaaaacaccg gcgcggttaa cggctgcgta cttgtttttg ggattgattt gcatggccct
     4081 gtcgaaatcg gccagggctg cttccggttt ctgggtgcgc agatagacgg cgcctcgatt
     4141 gttatagccc aggtcgctag ccggatccat tttgacggcc tgactgagat ccttgagggc
     4201 ccggtcgtag gatgctagtg cactataggc ggtaccgcgc tgcacgaggg cggcggcagc
     4261 atccttgcct ttgccgcctt ctgcctttat agcttcgtcg aattccggta tggctaattt
     4321 gtattggtcc cgctccagga aatcttcacc caattcgtag tgccgccgga actcaggact
     4381 ggctgcctgg caggccgtga agccggtcag catttgggtc aacaagccga cggcaataat
     4441 cttgacatgg gctgtcttcc ggatacggtt tggtttcatc actgattccc tgaaatttct
     4501 gctgagactg aaagaactgt ttcagggcac attttagcct ttgccgtatg acttgggctc
     4561 tttacatgag agcaatggcg ttaatggcaa aatttgaatg taaacgagcc ctttacatgg
     4621 tttcaactat agtctccaag tgggttcata acaggcattc tgggtatgtt gaaaaagagg
     4681 agaggcttgt ggacaaaaac tggggagtgc aggattcgga ctggcagttg cgcaaactgc
     4741 agcaggattc tctgcgcacc agtgatcgcg tgt
//
LOCUS       DAOQNZ010000099         4755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7628_length_4755_cov_3.458298, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000099 DAOQNZ010000000
VERSION     DAOQNZ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7628_length_4755_cov_3.458298"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2607)
                     /gene="alaS"
                     /locus_tag="PKW73_02725"
     CDS             complement(<1..2607)
                     /gene="alaS"
                     /locus_tag="PKW73_02725"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNA72225.1"
                     /translation="MALTGNEIRAKFVSYFEEKRGHLHLPSSSLIPDNPTLLLTSAGM
                     VQFVPIFLGQAKPTEPPRAVTVQKCARAGGKDSDIENIGRTSRHHSFFEMLGNFSFGD
                     YFKKEVIPWAWEFVTRDLGLKPENLYVTVFKGDEQNPPDDEAYEIWNKVVGVPAERIY
                     KMSRKDNFWGPPGPTGPCGPCSEIYYDRGPAYGPESFDDYCYKGVEGDRYMEFWNLVF
                     MELYKDENGNFSPLEKKNVDTGSGLERVALILQEKNNTFETDLFYPILEKVAQIAGIP
                     YRGGINNGPWNATSETKQDSYLKIIADHARCVTFLVADGVRTSNVGRGYVLRFIIRRA
                     ARFGRLLGITEPFIHTLVEEVAKIYGHFYKELISQKESTVTVIKEEEERFAKTIERGT
                     SLLNDLLAAEGKTLAGEDVFNLYATYGFPVELTAEIALEHGKDIDMEGFARARTKHEE
                     VSSVGKFNVIITGEEALGRVLKEHGATGFTGYGRLHDEGTILAILSQDGQLKPELTEG
                     EEADIILSTTPFYGESGGQLGDTGWLKNEAGILQVLNTKKHEGLHIHRVRAVSGTLAE
                     GDKVKAEVDGVRRKDTVLHHSSAHVFHAAVRQIFGKEVVQAGSQVGPAAMRFDFTLSK
                     QPTRQELAKVEALMNEWVRTASPVKTTEMSIDEAKQTGAIAMFGEKYGERVRVVEMGD
                     FSLEFCGGTHVNNTAEIGQIKIISEGSISQGVRRVEALSGEKAWNYISDQLRHLTEAT
                     DKLKIKPAELGAQIDRLQDNLKAREKQLQTLEEKLAMARIPELISQASQMGDTTVVAQ
                     AVQDISPASLKCVAENLKNRQGNLAVMLLATQPDGGVSLVTAFSQEMVKRGFNAGKIA
                     SEVAQILGGKGGG"
     gene            2836..3774
                     /locus_tag="PKW73_02730"
     CDS             2836..3774
                     /locus_tag="PKW73_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNA72226.1"
                     /translation="MKLEKVLVVHKKSTYQIQAQEHKEARFLKLIEAGSDVVRRVRLA
                     HEEHMETLELVEGELKRLGIEYQSLWRSQVPGIVEDVDLMISVGGDGTFLDASHALRN
                     VPILGVNSSCSSSFGHFCQANKDNFQDCLEAIIDERVKPIDILRLELSIDGIVIQELA
                     LNEVLVAHSSPAATSRYFLNVGALTEEHRSSGIWVSTPAGSTGSMRSAGGSVSPIASR
                     NFEYLVREPYTRPGEFFHLKNGLVSDGAEIRVTSQMRTGEVYIDGPHIIYPLSLGEEL
                     VIKASKFNLKAFIREDVNDIFDGPLAQTTKTCSGEM"
     gene            3778..>4755
                     /locus_tag="PKW73_02735"
     CDS             3778..>4755
                     /locus_tag="PKW73_02735"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNA72227.1"
                     /translation="MTEVPKSGLTASDKAAVLVAQLAAKTIRKLGLGLGSNLPGRIAR
                     KLSPSVLSHLSSQSAKGVVAVTGTNGKSTTSGILSEILRTAGFTFAHNRQGANLVTGI
                     TASLVESATWQSGISADMCLFEIDEAALPVVAREVKIGTVLVSNLFRDQLDRFGELDT
                     TAKLISAGISVNHSVAILNCDDPNVSQLVSDEKRFFYGIEDAADATDAAHAKNQELAY
                     CGKCGAEVAFRRVFYGQLGHWYCPACGNVRPAPQIVARDVEVFPTSSRFKLQIGATEE
                     SCVLPLPGLFNVYNALAAAACAHYLGVSNQAIRYGLKEYSTLFGRSEKLT"
BASE COUNT          950 a         1271 c         1311 g         1223 t
ORIGIN      
        1 gccgccgcct tttccaccaa gaatctgtgc tacttcgctg gcgatcttgc cggcgttgaa
       61 accgcgcttc accatttcct gactgaaggc cgttaccagg gaaactccac catcgggctg
      121 ggtggctaag agcatcacgg ctagattccc ctggcggttt ttgagattct ccgccacgca
      181 tttgagggag gccggggaga tgtcctgcac cgcctgtgcc accactgtcg tatctcccat
      241 ttggctggcc tgggagatca gttccggaat gcgggccata gccagtttct cttccagagt
      301 ttgcaattgc ttctctctgg ctttcagatt gtcttgtagc cggtctatct gcgcccccaa
      361 ctcggctggc ttgatcttga gtttgtccgt ggcttcggtg aggtgcctga gctggtcgga
      421 gatatagttc caggcttttt cgcccgagag ggcttcaacg cgtctgacgc cctgggagat
      481 actaccttcg gagataatct tgatctgacc aatttcggcc gtgttgttca catgggtacc
      541 gccgcagaac tccagggaga agtcgcccat ctccaccacc cgcacgcgct ctccgtattt
      601 ttcaccgaac atggcgatgg caccggtttg tttggcttca tcgatggaca tttccgtggt
      661 cttgaccgga cttgccgtgc gcacccattc attcatgagt gcttccacct tagccagttc
      721 ctgtctcgtg ggctgcttgc tcaaagtgaa gtcgaagcgc atggcggcag gtcctacctg
      781 ggaaccggct tgcactactt ccttgccgaa gatctggcgc acggcggcat ggaagacatg
      841 ggccgaggag tgatggagga ccgtgtcttt gcgtcttacg ccgtctactt cagcctttac
      901 tttgtcgcct tctgcgaggg tgcccgatac ggcccgtact ctgtgaatat gaagaccttc
      961 gtgcttcttg gtgttgagca cctgcaagat gccggcctcg ttcttgagcc agccggtgtc
     1021 gccaagctgt ccgcctgact caccgtagaa gggggttgtg ctgaggatga tgtcggcttc
     1081 ttcgccttcg gtcaactccg gtttgagctg accgtcctgg ctcaagatgg ccaggatggt
     1141 gccttcatca tgaaggcgac cgtagccggt gaagcctgtt gcaccgtgct ctttcagcac
     1201 gcgaccgagg gcttcttcgc cggtgatgat gacattgaat ttacccacgg aagaaacttc
     1261 ttcgtgtttg gttctggcac gggcaaagcc ttccatatcg atatctttgc cgtgctcgag
     1321 ggcgatttcc gccgtcagtt ccacggggaa gccgtaggtg gcatagagat tgaaaacatc
     1381 ttcaccggca agagttttgc cctcagccgc cagcaaatcg ttcaagaggc tggtgccccg
     1441 ctcgatagtc ttggcgaagc gctcttcttc ttctttgatg acagtgacgg tgctttcttt
     1501 ctgactgatc agttctttgt agaagtgtcc gtagattttc gctacttctt ccaccagggt
     1561 gtgaatgaaa ggttcggtga taccgagcag gcgaccgaag cgagccgctc ttctgatgat
     1621 gaagcgcaac acatagccgc gccccacatt gctggtgcgc acgccgtctg ctaccaggaa
     1681 ggtgacgcag cgggcatggt cggcaataat tttcaggtaa ctgtcttgct tggtttcgct
     1741 cgtggcgttc catggaccgt tattgatgcc gccccggtag ggaataccgg caatctgcgc
     1801 cactttctcc aggatgggat agaagaggtc ggtttcaaag gtattgttct tttcttgcag
     1861 gatgagcgcc acgcgctcga gaccggagcc ggtgtccacg tttttctttt caagtgggct
     1921 gaagttgccg ttctcgtcct tgtagagttc catgaacaca aggttccaga actccatgta
     1981 gcggtcgcct tccacacctt tgtaacagta gtcgtcgaag ctctccggcc cataagcagg
     2041 accgcggtcg taatagattt ccgaacaggg accgcagggt ccggtgggac cgggtggtcc
     2101 ccagaaatta tctttgcgcg acattttgta tatgcgctcg gccggcacgc ctacaacctt
     2161 gttccagatt tcgtaggctt cgtcatcggg tgggttctgt tcgtctccct tgaaaacggt
     2221 cacatagagg ttttcgggct tcagtcccag gtccctggtg acgaattccc aggcccatgg
     2281 aataacttcc ttcttgaagt aatcgccgaa gctgaaattg cccagcattt cgaagaagct
     2341 gtggtggcgc gaagtgcgac caatgttttc aatgtctgag tctttaccgc ctgctctggc
     2401 gcatttttgc acggtgacgg cgcggggcgg ttcggtgggc ttggcctgcc cgaggaaaat
     2461 cggcacgaac tggaccatac cggctgaagt cagtagtaaa gtgggattgt ccggaatcaa
     2521 gctggagctt ggtaaatgaa ggtgtccgcg cttttcttca aagtacgaca caaattttgc
     2581 gcggatctcg ttacctgtga gcgccatatg ctcctccttg gccaaagcat gtacgttgcc
     2641 ctgaagacgg tagtcctcga cggtaatctt ggacggtcat tcattgggca cttcagtcag
     2701 tcacttagac tgagggctga ttatagctta ttttcagaac ctctattgga cttgatgaat
     2761 aagcttgtaa tgcaggtcgt ccatctgcta atctgagttc tttgtaagct gcagcttagt
     2821 ttggtgtaat gcgatttgaa gcttgagaaa gttcttgtcg tccacaagaa gtccacttat
     2881 caaattcagg cccaggaaca taaggaagct cgattcctga agctcatcga ggccggctcc
     2941 gacgtggtca ggcgcgtgcg cctggctcat gaagaacata tggagaccct ggagttggtg
     3001 gagggcgaac tgaagcgcct cggtatcgag taccagtcgc tctggcgttc tcaggtgccg
     3061 ggtatagtcg aagacgtaga cctgatgatt tccgtcggtg gcgacggcac cttcctggat
     3121 gcctctcatg ctttgagaaa tgtccccatc cttggtgtca attcctcctg ttcttccagt
     3181 ttcggacatt tctgccaggc taacaaagac aactttcaag actgtctgga agccattatc
     3241 gacgaacggg tcaaacccat agacatccta cgactggaac tgtccataga cggcattgtc
     3301 attcaggaat tagctttgaa cgaagtgctg gtggcgcata gttcgccggc tgccaccagt
     3361 cgctatttcc tcaatgtggg agcccttacc gaggagcatc gctcttccgg tatctgggtc
     3421 agcactcccg ccggctccac cggctccatg cgttccgccg gtggttcggt aagcccgata
     3481 gccagtcgca atttcgaata tttagtgcgg gagccctata caagaccggg tgaatttttc
     3541 cacctcaaga atggtctggt gtccgacggc gccgagatca gagtcacttc tcagatgcgc
     3601 accggcgaag tctacataga cggtcctcat attatttatc ccttgagcct gggagaagag
     3661 cttgtcatca aagccagcaa gttcaatctg aaagctttta tcagggaaga tgtcaacgat
     3721 atcttcgacg ggcctctggc tcaaactact aagacgtgct cgggggaaat gtagagtatg
     3781 accgaggtcc ctaagagcgg cttgactgct tccgataaag cggcggtgct ggtggcgcaa
     3841 ctggccgcta agaccattcg caagctgggt ctgggactgg gctccaattt gcccgggcgt
     3901 atcgcccgta agctctctcc ttccgtattg agccatcttt cttcccagag cgccaaaggg
     3961 gtggtggccg tcaccggtac aaacggtaag agcaccacta gtggtatctt gtccgagatc
     4021 cttcgaactg ccggttttac ctttgcccat aatcgtcagg gagccaatct tgtcactggt
     4081 atcacggcca gcctggtgga gtcagctacc tggcaaagcg gtatcagtgc cgatatgtgc
     4141 ttgtttgaaa ttgatgaggc ggcgctgcct gttgtggcca gggaagttaa aataggcact
     4201 gtgctggtct ccaatctctt ccgtgatcag ttggatcgtt tcggtgaact tgatacaacg
     4261 gcaaagctga tatctgccgg tatttcagtc aaccatagcg tcgctatttt gaactgtgac
     4321 gatcccaatg tcagtcagtt ggtcagcgat gagaaacgct ttttctacgg catcgaagac
     4381 gccgccgatg ccaccgacgc cgcccacgct aagaatcagg agctggccta ttgcggcaag
     4441 tgcggggcgg aagtggcttt tcgcagggtc ttttacgggc agcttggtca ctggtattgt
     4501 cccgcctgcg gaaatgttcg cccggctccg cagatagtgg ctcgggacgt agaagtgttt
     4561 cctacttcct cccgcttcaa actgcaaata ggagccaccg aggaaagttg cgtcttgcct
     4621 ctgcccggtc tcttcaatgt ctataatgcc ctggctgcgg ctgcctgtgc ccactatctg
     4681 ggcgtgagca atcaagccat acgctacggc ttgaaagaat acagcacctt gtttggccgc
     4741 tccgagaagc tgacc
//
LOCUS       DAOQNZ010000100         4741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7664_length_4741_cov_3.539693, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000100 DAOQNZ010000000
VERSION     DAOQNZ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7664_length_4741_cov_3.539693"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1166)
                     /locus_tag="PKW73_02740"
     CDS             complement(213..1166)
                     /locus_tag="PKW73_02740"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNA72228.1"
                     /translation="MNYFQPAPYPDPAIEVLDSRFNKYRIGNASVERLATGCRWSEGP
                     VYFPAMGCLLWSDIPNNRILRYTEDSTGGHLSIFRQPSNYANGNAKDRAGRLYTCEHG
                     SRRVTRTEYDGTITVLADNFSGKPLNAPNDLIVDSLGHVWFSDPGYGIINDYEGFQAE
                     EELPRCLYRIDGNTGKLSVFADDLVRPNGLALSPDETKLYVVDSAMGHEGRACIKIYD
                     VNCESGKLKPGRIFADDFAPGTTDGIRFDCHGNLWCAMGWGDAGENGVRCYSPDGTLI
                     GKVHLPEICANLCFGGEKMNRLFMTASTSLYALYVGVRGLL"
     gene            complement(1180..2205)
                     /locus_tag="PKW73_02745"
     CDS             complement(1180..2205)
                     /locus_tag="PKW73_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72229.1"
                     /translation="MARPEGEVEEEELTLHFVPLLGSVLALEEGSLKRKLTEAEIIAV
                     RDRSVCIRMAKEHVEKLERERGLADIDAENVAAEWNRRRIEFTESYWPTLVLYVPTSG
                     PGTRACREILENAQKDSEYEGLEFEAKDHDKGLARHLIDQMSLLPPKADKSELSDLAR
                     HLQYFVVRSKPFNSDQAMEVASCYLRLIGKLLAAGALTVSVESSNLTHSRGAWQDLLE
                     RFQETPLVSLLFGFVQMPAVYKNEYFSVGMHSLGRPDFVINKETLRGLEYPEDKLDQA
                     AGELFQVLAYYLLDECPISQFRSGNTFSLSADSPQLKVTIEPASFFKESDIRSNPFGV
                     WRLSANS"
     gene            complement(2255..3595)
                     /locus_tag="PKW73_02750"
     CDS             complement(2255..3595)
                     /locus_tag="PKW73_02750"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA72230.1"
                     /translation="MKRNLLISTILGAVSISSLSLTACSEPKQPSGFIDKSGGMVLNL
                     DKLSPRPTVLGNFSEGLAVVKTEQGWGCLSKTGELVFDQPFKYLSPFAEGVAAYCTTD
                     GASRERWGYIYKNGTTAVRPAFAAAQKFSGDLAAVKLPEEHEDPKKAGRWCYIDKGGQ
                     QVIQDTFEAAEPFHDGLAVVKVNGKMGAINTAGNLIVPARYDVVYSASNGNIVAGRGT
                     GLSGENPDQVLDYMDAKGKVQFQKTIHGVTLKNLRPKLWVKFDPKETAEAAVESSWHR
                     PLSSFVSPGFSENKSIGQVDAKFAINQQFDARAFAGFYDYIYPVTGGYFVAYEDANGG
                     KMDYRGGVPEEAAGIWNSKTFRFFDAAPFSDGLGLVQESKDGRYGFVDKSAQFALKPT
                     YKYARSFRDSLALVGDAPIQTPEEYRQAELVKFSNGASKGPDESTLTESLSGLR"
     gene            complement(3698..>4741)
                     /locus_tag="PKW73_02755"
     CDS             complement(3698..>4741)
                     /locus_tag="PKW73_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNA72231.1"
                     /translation="ILNFDSCSMGNLNVAQAMQGEARHVVASAEVERSLNDADGQNVR
                     TTLGAILDQPDLSPQDFADRSIALARQGHNDDTGNLNPEASGTETLAHLDLSKYPQME
                     ATLDNLGEKLMTAASQPQNRQALVQIIDGVNPFASEGARISGAFEPSRRDLGLFLNAL
                     EQGLKENKITDPDGSLKQALEASRSALKSLVRDFHGENYKGYNEMAGVSLFLPGSDIL
                     SLDAHIAANSPLTDMLTSSTPGRKFARFDNRENLAESLNRSLEDMETLGDTKPLAQAV
                     EKVSSAKNETEFDQSLRQLQDLLRETQAGPLGRAFIEAQRQEMQERRSRLFEEQLNRL
                     PPHWQNFLNTMRS"
BASE COUNT          927 a         1217 c         1373 g         1224 t
ORIGIN      
        1 ggagatctgg tggttacttg aatttgcaaa agggcctgct gcctggcctg atcatcggcc
       61 ggcaagactt ccacatggtt tttagcttcg gccatccatt cttgtacgag agcccagccg
      121 ggttcttgcc ggttaatcag ttcttccaga cttctcagcg ccatcttctt cgatccttca
      181 gttgatttcc ttttgcactt tactctttta cttcacagca agccgcgcac tcccacatag
      241 agcgcgtaga gtgatgtgct tgccgtcatg aaaagccgat tcattttctc accgccaaaa
      301 caaaggttgg cgcagatttc aggcagatgc actttgccta tcagggtacc atccgggcta
      361 tagcagcgta cgccgttttc cccggcatcg ccccagccca tggcgcacca gagattgccg
      421 tggcagtcga atctgatgcc gtcggtggtg ccgggggcaa agtcgtcggc aaagattcgt
      481 cccggtttga gcttgccgct ctcgcaattg acatcgtaga tctttatgca ggctcgccct
      541 tcatggccca tggcactgtc taccacatag agctttgttt cgtcggggct tagtgcaaga
      601 ccattgggtc tgaccaggtc gtcggcaaag acggaaagtt tgcctgtgtt tccatcaatt
      661 cgatagaggc agcggggcag ttcttcctcg gcctggaagc cttcgtagtc gttgatgatg
      721 ccgtagcctg gatcggagaa ccagacatgg cccaggctat ctacaattaa atcattggga
      781 gcgttgagtg gtttgcctga gaagttatcg gccaggactg tgattgtgcc atcatattcg
      841 gtgcgggtga cgcggcggct gccgtgctcg caagtataga gccggccggc tctgtccttc
      901 gcattgccgt tggcgtagtt ggatggttgg cgaaagatgc tcaaatgccc gccggtagag
      961 tcttccgtat agcgcaggat gcgattgttg ggaatatccg accagagcag gcagcccatg
     1021 gccggaaaat atacaggccc ttcactccag cggcagcccg tggccagtcg ttctacggaa
     1081 gcattgccga tgcggtattt gttgaaacgg ctgtctaaaa cttcaatagc gggatcaggg
     1141 tagggagccg gctgaaagta gttcatgatc ttgtctcctt caagaattgg cactgaggcg
     1201 ccatactcca aagggattgg agcggatgtc cgattctttg aagaaggatg ccggttcgat
     1261 ggttactttc agttgagggc tgtcggcact caagctgaag gtattgccgg agcggaactg
     1321 cgagatgggg cattcatcga ggaggtagta agccaaaacc tggaagagtt cgccggcggc
     1381 ctgatcgagc ttatcctctg gatattcaag acccctgaga gtttctttgt taatgacgaa
     1441 gtcgggcctg ccgaggctgt gcatgcccac tgagaaatac tcgtttttat acacagccgg
     1501 catctgcacg aagccgaaga ggagggagac caggggtgtt tcctgaaagc gttccaggag
     1561 atcctgccag gcccctctgg aatgcgttaa gttggagctt tccaccgaga cggtgagggc
     1621 cccggccgcc agcaatttgc ctatcaggcg cagatagcag gaagctactt ccatggcctg
     1681 gtcgctgttg aaaggtttgc tgcgcaccac gaagtattgc agatgcctgg ccaggtcgct
     1741 cagttccgac ttatcggctt tgggcggcag aaggctcatt tgatctatga gatggcgcgc
     1801 cagacctttg tcatggtctt tggcttcaaa ttccagaccc tcgtattcac tgtctttttg
     1861 cgcattttcc agaatttctc ggcaggctct ggtgccgggg cccgaggtcg gtacgtagag
     1921 aaccagggtg ggccaataag actctgtgaa ttcgatgcgg cggcggttcc attcggcggc
     1981 aacgttctcg gcatcgatat ccgccagacc gcgctctcgt tcaagctttt ctacatgctc
     2041 tttagccatt ctgatacaga cagagcggtc tcttacggca atgatttcgg cttccgtcag
     2101 ttttcttttg agcgagcctt cttccagggc cagcacagaa cccagcaagg gcacaaagtg
     2161 caaggtcaat tcttcctctt cgacttcacc ttctggtctg gccattttgc ttgctgcttt
     2221 tgctttctct tatgggatgg ttgcctgcgt agatctagcg gagtccggag agagattccg
     2281 tcagggtgga ttcatcggga cctttagaag caccgttgga aaatttcacc aactccgctt
     2341 gtctgtattc ttccggtgtt tgaatagggg cgtcccctac cagggccagg ctgtctctga
     2401 aagagcgggc atatttgtaa gtgggtttga gggcgaactg ggcgctctta tcgacgaaac
     2461 catagcggcc gtccttgctt tcctgcacca gtccaagacc gtcggaaaag ggcgcggcat
     2521 caaagaagcg gaaggtcttg gagttccaga tgcctgctgc ttcttccggt accccgccgc
     2581 gatagtccat tttgccgccg ttagcgtctt cataggcgac gaagtagcca ccggtaacgg
     2641 gatagatata gtcgtaaaat ccggcgaagg cccgggcgtc gaattgctgg ttgatggcga
     2701 atttggcatc tacctggccg atggatttgt tttcgctgaa gccgggtgaa acgaaggaac
     2761 tgagcggtct gtgccaactg ctctctactg cagcttcggc ggtctctttg ggatcgaact
     2821 tgacccagag cttgggtctt aaatttttga gagtgacgcc gtgaatggtc ttttgaaatt
     2881 gcaccttgcc tttagcgtcc atatagtcca gcacctgatc aggattttca ccggagagac
     2941 cggtgcctct gcccgctact atattgccgt tgctggccga ataaaccacg tcgtatctgg
     3001 ccggcacaat caggtttccg gctgtgttta tggcgcccat cttaccgttc acttttacga
     3061 cggccagccc gtcgtgaaaa ggttcggccg cttcaaaagt atcttgaatg acttgttgcc
     3121 cgcctttgtc gatatagcac cagcgtccgg ccttcttcgg atcttcatgt tcttcgggca
     3181 gtttcaccgc tgccaggtca ccgctaaatt tctgagcggc agcgaaggcc ggtctaacgg
     3241 ccgtagtgcc gtttttatat atgtaacccc agcgttcgcg gctggcaccg tcggtggtgc
     3301 agtaggcggc tactccttcg gcaaaaggag acaaatactt gaagggttgg tcgaagacca
     3361 attcgccggt cttgctgagg cagccccagc cctgttcggt ctttactacg gccagacctt
     3421 cgctgaagtt tcccaggacg gtgggtctgg ggctgagctt gtcgaggttg agtaccatgc
     3481 cccctgactt atcgatgaaa cctgatggtt gttttggttc gctacaggca gtcaaactca
     3541 aggacgagat tgagacggcg cccaaaattg tagatatcag taggtttctc ttcatggcat
     3601 cccccggcga tagacggcta gtttatctta ttggcgaccc gggtcgtaat gtcgaatgag
     3661 acgaggttgc caggacgggg aagtagaatt ttgagcctca gcttctcatt gtgttcaaga
     3721 agttttgcca atgtggcggc aggcgattca gttgttcttc aaaaaggcgg ctgcggcgct
     3781 cctgcatttc ttgtctttgg gcttctataa atgccctacc tagaggcccg gcttgagtct
     3841 ctcgtagtaa atcctgcagt tgtctcagcg actggtcgaa ttctgtttca ttcttggctg
     3901 aggacacttt ctccacggcc tgcgccaggg gttttgtgtc gccaagggtt tccatatctt
     3961 ccagcgagcg gttcaagctc tccgctagat tttcccggtt gtcgaagcgg gcgaacttgc
     4021 gacccggcgt gctgcttgtg agcatgtccg tcagtggtga attggcggca atgtgagcat
     4081 ccagactgag tatgtcgctg ccgggaagaa ataaggaaac accggccatt tcgttgtaac
     4141 ctttgtagtt ttcgccatga aagtcgcgca ccaggctctt gagggcgctg cggcttgctt
     4201 ccagggcctg cttgagactg ccatcaggat cggtaatttt gttttctttc aggccctgct
     4261 cgagggcatt gaggaagagg ccgagatcgc gccttgaggg ttcgaaggcg ccgctgatac
     4321 gggccccctc gctggcgaag gggttgacgc cgtcgatgat ttgcaccagg gcctgtcgat
     4381 tttgcggctg cgatgcggcc gtcatcagtt tctctcccag gttgtcgagg gttgcctcca
     4441 tttgtgggta ttttgagagg tccaggtggg cgagagtctc cgtaccggaa gcttcgggat
     4501 tgagattgcc ggtgtcgtcg ttgtgtccct ggcgggccag cgcaatggag cggtcggcaa
     4561 agtcctgagg gctcaagtcc ggctggtcga ggatggcacc gagggtggtg cggacatttt
     4621 gaccgtcggc gtcgttcagt gaacgttcca cctcggcgga tgctaccacg tgtctggctt
     4681 cgccctgcat ggcctgggct acattgagat tgcccatgga gcaggagtcg aagttgagaa
     4741 t
//
LOCUS       DAOQNZ010000101         4721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7711_length_4721_cov_4.124518, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000101 DAOQNZ010000000
VERSION     DAOQNZ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7711_length_4721_cov_4.124518"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PKW73_02760"
     CDS             <1..1168
                     /locus_tag="PKW73_02760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNA72232.1"
                     /translation="QADAYFTDKEVTDAKAVVAKLKELVEDSRKQGFDRQKSQALLAC
                     RSNFSSSKVVESANLLLDELVLSRSASALAARLGLSENRKDLLDSFFNGIFAFVEADL
                     VGLVMAASATPWAAFHCDKVVSGSYKKLTERLSTELELKSELQIELRGELTEGTGKDM
                     GSVQIVPVSYKGRALGALVVASKDRNAFSDQSLAFGEMLGQSLPTAFELLLARETISD
                     MQTREAYRASIDSLTGLYNLEFLVGFLQQQLLFSFRQRLPVAVVIVDIDAFSKVNQEH
                     GFEFGDGALMTIANRLLNITRSSDLIARYGGDQFAVVLPNTDVAGARVLAEKVRSDVE
                     SLDFSNGSKGHRITVSVGCASFNMEDLNPETILRDAKLALRKAKDEGRNRISAG"
     gene            1245..3086
                     /locus_tag="PKW73_02765"
     CDS             1245..3086
                     /locus_tag="PKW73_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72233.1"
                     /translation="MLTCGRVQNVAIIILTAIMILVSSPALALDGQGESEGAPEAVLN
                     LDLCTLAYQLYHQSLCLPLDPWYDLMSRVGSDRRENICRFTHEYAARLGAAGPVGQSP
                     EAGFYSGPNAARNWSGTNLNLDPILTNYKFIDARNPAFTRDGERYLAVKAPAYLSQNI
                     RVIDGVRYRAKPAGFPSNDVEMFRVREYPTGEDHLVVFEGGTGIVGATEPAWSLMGFV
                     LMHKTATGYDAHIVFRGSRSGASLAKTVLKAQDVVGDAKGNPDWITDLRGSKQIPQPL
                     ISKVGKVTEGFANSLPTMLGPVSQCCKYLSEKYPAPEHIFVTGHSLGAGLATQFASSV
                     LQGGYGAELTRQVPGWAWDKIMLVAYAQPIPGDPEWAQEFDKLSPSSQHYWVEGDMVV
                     EATSSSLVNLFIDKGEHAGIQKKLAAVANCQDNPHEVFVIRAALLRDLSSNFAPLVSR
                     LGAENTWGYYENISKMVTGQAVSYVFPGAPAPVIVSQSNLRQVLSNCNFGQQFGNWLE
                     QVYARMIADKSSYIGPKFQSTLDERRQLVLNLVARMRQPLPGDMNQAIDCLVEDFKLI
                     DGNLGLTEEEQWIYSAMLLGRLETSPLTLTDLQSRPETKAGLESAPN"
     gene            3337..3945
                     /locus_tag="PKW73_02770"
     CDS             3337..3945
                     /locus_tag="PKW73_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72234.1"
                     /translation="MARKSDDHLSATRSFKFNTRLKPLFAREDLPERWLDSPIEEFIA
                     AHNFEKPVEEGTTPKLLVVSCIEFRFMPQIPRNFAYMIRTAGGRITQIPGSEFALAYI
                     LANGVRHIVTVGHNDCGMTKVEAFKPRLVEALAEQGWEQDHAKNFVEAHADRFAIDDE
                     IDALESEYVRMRSLFQKVEIAPLFVSLSSSRLHLPSWFEKYK"
     gene            complement(3963..>4721)
                     /locus_tag="PKW73_02775"
     CDS             complement(3963..>4721)
                     /locus_tag="PKW73_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72235.1"
                     /translation="LLAECVPGGTTTAALVLSLLLEESADMAALCSSSLVPSQSVQSG
                     TESSQAALVHNFDKTVFLEKELLALAQSRGAALSDLKQECRLNPLKAVAMAGDPMQAY
                     AAAYLLKRSQSGLVIAAGGSQMLAVYQLARRLAGEEKILARYLSGNFHAAAFLKNCLV
                     ATTSYVAFDRAARIPELATLCLAPMVSIDPGLSKSCHSGLRAYEDGHVKEGVGAGAAC
                     LLAYTMGKLEQDQLLALIDDTYKCLVQPEVLQPG"
BASE COUNT         1020 a         1220 c         1307 g         1174 t
ORIGIN      
        1 ccaggcggat gcctatttca ccgacaaaga ggtcacggat gccaaagcgg tcgtggctaa
       61 attgaaggaa ctggtggagg actcgcgtaa gcagggcttt gatcggcaga agagtcaggc
      121 attgctggcc tgccgcagta acttttcctc ttcgaaagta gtggaaagtg ccaatttgct
      181 cctggatgaa ctggtcctct ctcgctccgc ttcggctctt gccgcgcgtc ttggtctttc
      241 tgagaaccgc aaagatttgt tggattcctt ctttaacggt atcttcgcct ttgtggaagc
      301 cgatctcgtc ggtctggtaa tggccgcttc cgctacgccc tgggccgcct tccattgcga
      361 caaggtggtg agcggcagct ataagaagtt gacggagcgc ctctctacgg aactggaact
      421 gaagagcgaa ttgcaaatcg aactgagggg tgaactgacc gaaggcaccg gtaaagacat
      481 gggctcggtg cagatcgtgc ccgtatccta taaagggcgg gccctgggag ccctggtggt
      541 agcctccaaa gacagaaatg ccttctccga tcagtccctg gctttcggcg aaatgctggg
      601 gcagtccctg ccaaccgcct tcgagctttt gcttgcccgg gaaactattt ccgacatgca
      661 aacccgcgaa gcttatcgcg cttctataga ttctctcacc ggtctatata atctggagtt
      721 cctggttggt ttcttgcaac aacaactgct cttttccttc cgccagcgct tgcctgtggc
      781 cgtcgtgatc gttgatattg atgccttcag caaagtgaac caggaacacg gctttgagtt
      841 cggtgacggt gctcttatga ccattgccaa tcgcttgctc aatatcaccc gttccagcga
      901 tttgatagcg cgctacggtg gtgatcagtt tgccgttgtt ctgccgaaca ccgatgtagc
      961 cggcgcccgc gtcttggccg agaaagtacg ttctgacgtg gagtcgctcg acttcagtaa
     1021 cggtagtaag ggtcatcgga ttacagtttc ggttggttgc gccagcttta atatggaaga
     1081 cttgaatccg gaaacaattt tgcgcgatgc caagcttgcc ctgcgcaaag cgaaagacga
     1141 aggacgcaat cgtatttcgg ctggttagct gaataagttg gcattagctg cctgtacctg
     1201 ctgaagtttg tcttcagtca aaatcccaat caaggaaaag taatatgctc acctgtggac
     1261 gagtccaaaa cgtcgccatc atcattttga cagccattat gattttggtt tcctctccgg
     1321 ccctggctct ggatgggcag ggtgagtcag aaggcgctcc cgaagcggtc ctgaatttag
     1381 atctgtgcac ccttgcctac cagctctacc atcagtctct gtgtttgccc cttgacccct
     1441 ggtatgacct gatgtcgcgg gtgggaagcg ataggcgcga gaatatttgt cgatttaccc
     1501 atgaatatgc agcccgactt ggcgccgccg gtcccgtggg gcagtcaccg gaagcgggtt
     1561 tttacagtgg tcccaatgca gcgcgcaact ggtcgggcac caacttaaac cttgatccca
     1621 ttctgactaa ctacaaattc atagatgcca ggaacccggc ttttacccgt gacggtgagc
     1681 gttatcttgc cgtcaaggcg ccggcttatt tgagccagaa tataagagtg attgacggtg
     1741 tgcgctatag agccaaaccg gccggctttc cctcaaatga tgtggagatg ttccgggtgc
     1801 gcgaataccc gaccggtgaa gatcatctgg ttgtttttga gggcggcacg ggcattgttg
     1861 gcgccaccga gccggcctgg agtctcatgg gtttcgtttt gatgcacaag accgccaccg
     1921 gatatgatgc gcacatcgtt tttcgcggca gtcgtagcgg cgcctccctg gcgaaaactg
     1981 ttttgaaggc gcaagatgtc gtcggtgatg ctaagggaaa tcccgactgg attaccgatc
     2041 ttagaggaag caaacaaatc cctcaaccat tgatcagcaa ggtcggtaag gtaaccgaag
     2101 gctttgccaa ttctctgccg actatgcttg ggcccgtaag ccaatgctgt aaatatctct
     2161 ctgagaagta cccggctccg gagcacatct tcgtaaccgg tcacagtctg ggagccggtt
     2221 tggctactca gttcgccagc tcggtattgc agggcggcta cggtgcggaa ctgaccaggc
     2281 aagtgcctgg ctgggcctgg gataagatca tgctggtggc ttacgcacag cctatacctg
     2341 gcgacccgga gtgggcgcaa gagtttgata aactctctcc ctcctctcag cattactggg
     2401 ttgagggaga catggtagtg gaagccactt ccagcagtct ggtcaaccta tttatagata
     2461 agggcgaaca tgccggtatt cagaagaagc ttgctgcggt agcaaactgt caggacaacc
     2521 cccatgaggt gtttgtgatt cgtgccgcgc tactgcgtga cctttcttcc aactttgcgc
     2581 ctctggttag caggcttggt gcggagaata cctggggcta ttatgaaaat atctccaaaa
     2641 tggttaccgg gcaggcggtc agctatgttt ttccgggcgc accggctccc gttattgtct
     2701 ctcagtccaa tctcaggcag gtattatcca actgcaattt tggccaacag ttcggcaact
     2761 ggctggagca ggtttatgcc agaatgatcg cagataagag cagttatatc ggtcctaagt
     2821 ttcagagtac ccttgatgag cggcgccaac tggtactgaa tctggtggcg cgcatgaggc
     2881 aacctttacc cggcgatatg aaccaggcta ttgattgtct ggtggaagat tttaaactga
     2941 tcgacggcaa cctcgggctt acggaggagg agcagtggat ttattcagcc atgcttctcg
     3001 gaagactgga aacctctcct ttgactctaa cggatctgca atcgagaccg gaaacaaaag
     3061 ccggccttga atcggcgccc aattaagcgt ctgcttgatt aactagcgag ccggtccggt
     3121 gttatttaag aaaagtagtc gcgagcgaaa ttcgccataa tttcgctcgc gactactttt
     3181 tcgccatgaa gcaccatcct cgggtcaggc gctttattta tttacttatt gatgcgaatt
     3241 gttcgtattc tcacggacct acccggtaaa ggctctgctg aatttacacg ggggttcggt
     3301 ataatatctg ctcccgcatc aaggcaagga aaccatatgg ccaggaaaag tgacgaccat
     3361 ctgagcgcca ctcgctcgtt caagttcaat acgcgcttga aacctctttt tgcaagagag
     3421 gatttgccgg agcgttggct tgatagtccc attgaagagt tcatcgccgc tcacaatttt
     3481 gaaaagcctg tggaagaagg caccactccc aagttgcttg tagtttcctg catcgagttt
     3541 cgcttcatgc ctcagattcc ccgcaatttt gcctatatga tacgcaccgc cggtggtcgc
     3601 attactcaaa ttcccggttc tgaatttgcc ctcgcttaca ttcttgctaa tggcgtcagg
     3661 cacatcgtta cggtcggaca caacgattgc ggtatgacca aagtggaagc tttcaagccc
     3721 cggctggtgg aagctctggc tgagcagggg tgggagcagg atcatgctaa gaactttgtg
     3781 gaagcccatg ccgatcgctt tgctatagat gatgagattg atgctctgga gtctgaatac
     3841 gttcgtatgc gcagcctctt tcaaaaggtg gagattgcgc cgctcttcgt ctcactatcc
     3901 agttcccggc tgcacttgcc gtcctggttc gagaagtata agtaagttgt ttgcgtttgt
     3961 agtcagcctg gctgcagcac ttccggttgc accaggcatt tataggtgtc gtctataaga
     4021 gcgagtaact gatcttgctc caattttccc atggtgtaag ccagtaagca ggcggctccc
     4081 gctcccacgc cttccttgac gtgaccatct tcgtaggccc tcagcccgct gtggcaactc
     4141 ttgctcagtc caggatcgat gctcaccatg ggggccaggc aaagggttgc cagttcgggt
     4201 atacgagcgg ctcggtcgaa ggccacatag ctggtggtgg caaccaggca gtttttaagg
     4261 aaggcggcgg catggaagtt gccgctcaag tagcgggcca gaatcttctc ttcgcctgct
     4321 agacgccttg ccagttgata gacagccagc atctgactgc cgccggcggc aatgaccaga
     4381 cctgattggc ttcgtttaag cagataggca gcggcgtagg cttgcatggg gtcgccggcc
     4441 atggctaccg ctttgagcgg attgaggcgg cattcttgct tgaggtccga gagcgcggcg
     4501 ccccggctct gagccagggc taaaagttcc ttttccagaa agacggtctt gtcgaagttg
     4561 tggacaagcg ctgcctggct gctttctgtg ccggattgga ccgactggct cggcactaaa
     4621 ctggaggagc agagggcggc catatccgcg ctttcctcca ggagcaaact caagaccagg
     4681 gcggcagttg tggtgccgcc cggcacgcat tcggcaagca g
//
LOCUS       DAOQNZ010000102         4719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7723_length_4719_cov_3.527659, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000102 DAOQNZ010000000
VERSION     DAOQNZ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7723_length_4719_cov_3.527659"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKW73_02780"
     CDS             complement(<1..499)
                     /locus_tag="PKW73_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA72236.1"
                     /translation="MAKILVVEDDLDLARVVSRWLGVEGHIIDMAGDLTEARHMLENS
                     EYDLIILDRMLPDGEGADLVRAYRLEGCPTPVLFLTGKRSIEEKVEGLESGGDDYLTK
                     PFHGKELMARVSALLRRPVKIASAVLKVGRLELVPERRSIKRDGQEIALVAREYSLLE
                     YLLRHP"
     gene            complement(531..2285)
                     /locus_tag="PKW73_02785"
     CDS             complement(531..2285)
                     /locus_tag="PKW73_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNA72237.1"
                     /translation="MFELTIFGVLWSMVERLDQARQAEAHGRAMAASINRIGTLHMER
                     VTVLILRPFNRGQSDMLRNQARERVRDINEEVKNMERLTVGYPNEYQLCKRLNAAFEK
                     LGAMFTTSSDTTTTRDEASRALTLMGVKEGMEEVNSIIKVLSQQSVGDLQGKQDLLLK
                     YDNELKYALALAASLSVALALGMAAYLNRSTARRFERLTENTARLAANQSPAAPITGT
                     DEIADIDRLCQSLYQSLVELRARERAVLDNAIDVICGIDREMRFIEVNEAACRLWGYE
                     KDELLGMRAVEVLEEGQAETFLKAFESVQPGYKARVVELSVRTFEGKVVETEWCITCT
                     CEDSSYYLVIHDISQRKQMERMKQEFAAMVSHDLRTPLNSVLLTLELLQQEGAAVLSG
                     DSLTDLQAAAGNVNRLLSLVNNLLDLESMADGQMRLFKEKCRLEDLVDGAVNSVEAIG
                     RQRAITIDRRVEPVEYIECDQERVIQVLVNLLGNALKFSPQNSRVLLRGRKQGGFYRI
                     EVRDRGRGIPEDKIAYVFDRFYQVKGKDDRGLKGTGLGLSICKSIVELHGGKIGVFAG
                     EDGGCVFWFTLPLMKEEA"
     gene            2498..4114
                     /locus_tag="PKW73_02790"
     CDS             2498..4114
                     /locus_tag="PKW73_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA72238.1"
                     /translation="MSSSSPPAQKTSLYVPALLMVAALLYLPWLGSYGPLDPTDSFFI
                     EAAREAVETKQYLLPMINYEYWLDKPILYFWAVSGSFHLLGINAFAGRIFTALTAIAL
                     GLVVYFGSRGFIGKRQAFLAALIFYAFPLSSALGHVSLTDMPLTLFMSGGLYGLFHYH
                     HKKNQKALWLGYFSLAMAFLCKGPIAIILVGAILCLHLAATSASAGAFFSSFLNLRPL
                     AGFLVILGLNLPWYAAATIGTKGAFFKDFFITQNFGRMVGTVNHQQPFWFYIPVFIGG
                     LFPFNLLLLSAPALKLRHLRQRLSLNEGTAFMLFQAIWAIFVLVLFSIIKTKLPTYIL
                     PAMAPLAVLCARAAALTVRLGRAKRLLPAALITLVTAVGAAVAAYKVTGWARLLLMQS
                     LPLLVLIGALSLAQLWFILRGKTQKALLCLLTLGVISVGIAVPQAHIAFYEDRQVPVD
                     ALTLKVLGQGGSLATIIRSEPSFSYWLHKHIPCVKTPAEARSFLSTAQGPHWVLAPKE
                     VLNNIEWFGSADKIEVAGKTKKWTLFKIKP"
     gene            4270..>4719
                     /locus_tag="PKW73_02795"
     CDS             4270..>4719
                     /locus_tag="PKW73_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72239.1"
                     /translation="MTRETLTPKKAMSSRLVLLALAIALFVLSPFKLMSQAEQAMASI
                     CVKEAMACCIKEADATSPVAGSQTPAGSPARALSPERAALEAVSENCCCCSEAPVIPG
                     NLEAGTTPPSQNTKFVSIKSSLPLAVAGLSNLAPPPDLVTASSPVTTA"
BASE COUNT         1055 a         1410 c         1170 g         1084 t
ORIGIN      
        1 gggggtgccg caggagatat tcgagcaaag aatactccct ggccaccagg gcaatctcct
       61 gcccgtctct tttgatggaa cgccgctccg gcaccagctc aagacgcccc accttgagca
      121 cggcggaagc aatcttcacc ggtcggcgca agagcgccga gactctggcc atcaactcct
      181 ttccatgaaa aggcttggtc aggtaatcgt cgccgccgct ttccagcccc tccacctttt
      241 cctcaataga gcgcttgccg gtgaggaaaa gcaccggcgt ggggcagccc tccaggcgat
      301 aggcccgaac cagatcagcc ccctcgccgt caggtagcat gcgatcgagg atgatcaagt
      361 cgtattcgga attctcgagc atgtgacggg cttcagtcaa atcaccggcc atatcgatga
      421 tatgtccttc cacccctaac caacgagaaa ctacccgggc caggtccaga tcatcttcca
      481 ccaccagaat cttagccata ccgctcctca aagctgtcgg aaactatcag ttacgcctct
      541 tccttcatta gcggcagggt gaaccagaaa acacaacccc catcctcgcc ggcgaaaacg
      601 cctattttgc caccgtgcaa ttctacaatc gatttgcaaa tagaaagccc taaaccggtt
      661 cctttcagtc ctctatcatc cttccccttg acctggtaaa agcgatcaaa gacataagca
      721 attttatctt caggaatacc gcgccctcta tctcgcacct caattcgata gaagccgccc
      781 tgcttgcgtc cacgcaagag cacgcgggag ttctggggcg agaatttgag agcattgccg
      841 aggagattga ccaaaacttg aatgacgcgc tcctggtcgc actcgatgta ttcaaccggc
      901 tccacgcgcc tatcgatggt gatagcccgc tgacgcccga tggcctccac ggaattgacc
      961 gcaccgtcca ccagatcctc gaggcgacat ttctctttga aaagccgcat ctgcccatcg
     1021 gccatgctct ctaaatcaag cagattgttg accagggaaa gcaacctgtt tacgttgccg
     1081 gcggcggctt gcaggtcggt gagggagtcc ccggataaaa ccgccgctcc ttcctgctgc
     1141 aaaagttcca gagtaagcag aaccgagttg aggggagtgc gcagatcatg ggagaccatg
     1201 gcggcaaatt cctgcttcat gcgctccatc tgtttgcgct gggaaatgtc gtggatgacc
     1261 agataatagc tgctgtcctc acaagtacag gttatacacc attccgtttc caccactttg
     1321 ccttcgaagg tgcgcacact gagctccacc accctggctt tatagccagg ctgcacagac
     1381 tcgaatgctt tcaagaaggt ctcggcctgc ccttcttcca gcacttccac ggctcgcata
     1441 ccgagaagct cgtctttctc ataaccccag agtcggcagg cggcctcgtt cacctcgatg
     1501 aagcgcatct ccctgtctat gccgcaaatt acatcgatgg cattatccag taccgcccgc
     1561 tctcttgctc tcagctctac cagcgactgg taaagactct gacagagcct gtcgatgtcc
     1621 gctatttcat cagtaccggt aatgggcgca gccggagact gattggccgc caaccgggcc
     1681 gtgttctcgg tcaacctctc aaagcgcctg gcggtgctgc gattaagata agcagccatg
     1741 cctaaagcca gtgccacact cagagaggcc gccagagcca gggcatattt caattcattg
     1801 tcatacttga gaagcaggtc ttgtttgccc tgtaaatcgc cgacgctctg ctgagagagc
     1861 actttgataa tgctgttgac ctcctccatg ccctccttca cccccatgag agtgagggcc
     1921 cgactggctt cgtcccgagt agtagtggtg tcgctggagg tcgtgaacat ggcgccgagc
     1981 ttttcaaaag cagcattaag gcgcttgcag agctgatatt cattgggata accgacagtc
     2041 aaacgctcca tatttttgac ttcttcattg atatcacgca ctctttcccg ggcttgattg
     2101 cgaagcatgt ccgactgacc acggttgaaa ggacgcagaa taagaactgt aacccgctcc
     2161 atgtgcaggg tgccgatacg attgatactg gcggccatgg ctctgccgtg cgcttctgct
     2221 tgcctggcct ggtccaaacg ttcgaccatg gaccagagca caccgaaaat tgtgagctcg
     2281 aacacgacag gaaaggccgc taccagtaaa attttctgag aaagtcgcat gatcggcaat
     2341 attctaaact gttttagaag ctgttactgt atgtagaggc ggtcaaacca ccgaccgccc
     2401 ggcccgccca ccggccgacc ggttagaatt ggcggcactg cccccgcagt tcagaccggt
     2461 ccatgagtag atatcgtttc agtgtaagtt tggaagcatg agtagttcca gtccgccagc
     2521 ccaaaagacc agcctgtatg tgccggcctt gcttatggtg gcagcactgc tttacttgcc
     2581 ctggttgggc agctatggtc ccctcgaccc caccgacagt ttcttcattg aagcagcccg
     2641 ggaagctgtg gaaaccaaac aatacctatt gcccatgatc aactacgaat actggcttga
     2701 caagccgatt ctgtatttct gggcagtttc cggcagcttt cacctgctcg gcataaacgc
     2761 cttcgccggg cgcattttca cggcacttac agccatcgcc ctcgggctgg tggtttattt
     2821 cggctcccga ggcttcattg gtaagcgtca ggcttttcta gccgccctca tcttctatgc
     2881 ctttccctta agctcggcgc tggggcacgt ctcgctcacg gatatgcctc tcacactgtt
     2941 tatgagtgga ggactgtacg gactctttca ctatcaccac aagaaaaatc agaaagcgct
     3001 ctggctgggc tatttctctc tggccatggc ctttctttgc aagggaccga ttgccataat
     3061 tctcgtgggc gccattctct gcctccatct ggcggctaca agtgccagtg cgggcgcctt
     3121 cttctcttct ttcctgaatc tgaggccgct ggctgggttc cttgtcattc tggggctcaa
     3181 cttaccctgg tatgccgccg ccaccatcgg cacaaaaggc gcctttttca aagacttctt
     3241 catcacccag aatttcggac gcatggtggg caccgtcaat caccagcaac ctttctggtt
     3301 ctacattcca gtcttcatcg gcggactctt tcccttcaac ctgctactgc tgtcggcccc
     3361 ggccctgaaa cttcgccact taagacagag gctcagtcta aacgaaggca ctgccttcat
     3421 gctcttccag gcgatctggg cgattttcgt actcgttctc ttcagcatca tcaagaccaa
     3481 attgcccacc tatatcttgc cggcgatggc tccactggct gttctttgcg caagagccgc
     3541 cgccctgacc gtgcgcctgg gaagggctaa gcgactcttg ccggccgccc tcatcaccct
     3601 ggtaaccgct gtgggtgctg ccgttgcagc ttacaaagtg accggctggg cacgcctgct
     3661 gctcatgcaa tcactaccgc ttctggttct gatcggagcc ttaagcctgg cacagctctg
     3721 gtttatcttg agagggaaaa cccagaaagc gctcctctgt cttttaacgc tgggcgttat
     3781 cagcgtcggc attgccgttc ctcaggcgca catagccttc tacgaagatc gacaggttcc
     3841 ggtagatgcg ctcaccttga aagtgctggg gcagggcggc agcctggcaa cgataattcg
     3901 ttccgaacca agtttctcct actggctgca caaacacatt ccctgcgtga agacccctgc
     3961 tgaagcaaga agcttcctca gtaccgccca gggaccacac tgggtgctgg cgcccaaaga
     4021 ggtattgaat aatatcgaat ggttcggcag cgccgataaa atcgaagtgg ccggtaagac
     4081 aaagaaatgg accttgttca aaataaaacc gtaaatactg cgggcgaact ttatatgtct
     4141 gtaaaccctg acggccgctt ggtaaagaac tcatcggtag aggtaaaaaa ctacggcttt
     4201 atacttgtca ggcattgcgg gatttgttga cgcgggtaga atgcaagtaa gggctcagga
     4261 aatgagatca tgaccagaga gacactgaca ccgaagaaag ccatgagcag cagacttgtg
     4321 ctcctggctc ttgctatcgc cttgtttgtc ctctctccct tcaagctcat gagccaggcc
     4381 gaacaagcta tggcttccat ttgcgtgaaa gaggccatgg cctgttgcat caaagaagcc
     4441 gatgccacga gcccggtagc cggttctcag accccggcag gaagtcccgc cagggcactc
     4501 tcgcctgaaa gagcggcctt agaagcagtc agcgaaaact gctgctgttg ctccgaagca
     4561 ccggtcattc ccggcaatct ggaagcggga acaacaccgc cctcgcaaaa tacaaagttt
     4621 gtctcaataa aatcgagtct gcccctggcc gttgccggtc tgagcaacct cgcgccacct
     4681 cccgacttag tgactgcgtc cagtccggtg acgacggcg
//
LOCUS       DAOQNZ010000103         4712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7741_length_4712_cov_4.640971, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000103 DAOQNZ010000000
VERSION     DAOQNZ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7741_length_4712_cov_4.640971"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKW73_02800"
     CDS             complement(<1..442)
                     /locus_tag="PKW73_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72240.1"
                     /translation="MPSLPEIARKLVLTLSTVGGCLSLPLLAAAGAQPTEWLVVQSHE
                     SKTDAVIHVTHDAVKLESKALGCTLLTRAPDWKVHCYRPLEKIEWIGDMGQFSGMVMS
                     NPYALPKPTLRGPAPRCVGETEMNGLKCKIYKGFASEVYCADSIS"
     gene            675..1211
                     /locus_tag="PKW73_02805"
     CDS             675..1211
                     /locus_tag="PKW73_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72241.1"
                     /translation="MKQTQEYLPQPEGAISTSTSEQTQASERLLQEVGKPDQPSGQGC
                     QLEKLCSVRYANTDEMKIPHATEPVLKLFKSGDSFSQDGNSQRLTFAEGYSVEVGNKG
                     QFSVRDASGNRVQADEIMIKPGPGVTEPQRHYAFGDLMTASISKDGQVKLQTGTGAVL
                     VLDRRGMTSMSRDNKQLF"
     gene            1244..1954
                     /locus_tag="PKW73_02810"
     CDS             1244..1954
                     /locus_tag="PKW73_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72242.1"
                     /translation="MENYFLDLADKDTIELSIAAEQVYGLVCRNDFSKPGFALIRLPE
                     STTSSIQRMLMVNLKDHLAALFQTERGTGLSWFNMTRFDQKNTTKFHRDGAPSESLLI
                     LGYEPSKVHSEISMADFSRCAHELGITPDQFLEDFNPMYEKGLEKLAPFISPVSKFDP
                     AGFQILVINNSSNPLQPGKKRWQGVLHSALVSNGTGTRVINSTCVAPTDSAKTSAVTE
                     KQVTEFLYENKLEGSYSL"
     gene            2773..2844
                     /locus_tag="PKW73_02815"
     tRNA            2773..2844
                     /locus_tag="PKW73_02815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2805..2807,aa:Pro,seq:tgg)
     gene            2850..2922
                     /locus_tag="PKW73_02820"
     tRNA            2850..2922
                     /locus_tag="PKW73_02820"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2883..2885,aa:Ile,seq:gat)
     gene            3131..3206
                     /locus_tag="PKW73_02825"
     tRNA            3131..3206
                     /locus_tag="PKW73_02825"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3164..3166,aa:Asn,seq:gtt)
     gene            3279..3350
                     /locus_tag="PKW73_02830"
     tRNA            3279..3350
                     /locus_tag="PKW73_02830"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3309..3311,aa:Trp,seq:cca)
     gene            3384..3469
                     /locus_tag="PKW73_02835"
     tRNA            3384..3469
                     /locus_tag="PKW73_02835"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3418..3420,aa:Tyr,seq:gta)
     gene            3557..3916
                     /locus_tag="PKW73_02840"
     CDS             3557..3916
                     /locus_tag="PKW73_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72243.1"
                     /translation="MRTLQATGGRIEIDDSEPSHCQCFLITSGSDKYLLGWQPLYYLK
                     EKLLRALKEEPADPKKLLFAGAFEPLRFTLYLTRQGDTRRILVQDDHLLASGDGILHI
                     LELSQEDVDSWVLTLHR"
     gene            complement(3924..>4712)
                     /locus_tag="PKW73_02845"
     CDS             complement(3924..>4712)
                     /locus_tag="PKW73_02845"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72244.1"
                     /translation="LQFLAFAAPGGAQETTWSQDLEVGSMAYRQGKYAVAELAFSEAL
                     TKAEKFGAEDVRVATTLNNLALVCETQGKYVEAEPLYKRALAIYEKVRGAEHPDLATA
                     MNNLALVYEAQGKYTEAEPIFQQALTIQKKCFGAEHASVANTMANIAALYADQKKYTE
                     AEQLYGESLAIEEKVLGPDNVSLAKNYNNLAVVYKKQGKLKEAEELYKKSLLIYEKAL
                     GPDHPEVAILLENYAALLKLAGRDAEANELAERVKAIREKLDKQ"
BASE COUNT         1277 a         1243 c         1109 g         1083 t
ORIGIN      
        1 ccgaaataga atcagcacaa tagacttcgc tggcaaagcc cttataaatc ttgcatttca
       61 agccgttcat ttcggtttcc cccacgcagc gcggcgcagg gcccctgagg gtcggtttcg
      121 gcaaagcata tggattggac atgaccatgc cgctaaactg ccccatatcg ccaatccatt
      181 cgattttctc taagggacgg tagcagtgca ccttccaatc aggggccctg gtcaaaagag
      241 tgcatcccaa tgccttcgac tccagcttca ccgcgtcatg ggtaacatgt atgaccgcat
      301 cggtcttact ctcatgactc tgcacaacca gccactccgt cggctgcgcg ccggcagcag
      361 ccaggagggg aagactgagg caaccgccga ctgtagagag cgtcaggaca agctttctgg
      421 caatttccgg caaagaaggc attcttcaga accactccat ccacatacta aagtctggcc
      481 cactttgcgg aattcagcaa gccttacccg accaggaagg attaaatatc agggctgagt
      541 gcaaacggac cacgagatat gccggcgact gcagtatgtc aacctgatgt caaccagtcc
      601 ccctaaattg agggtgagag agccgcaacc ctcagacgcc tctctcgccc cttttcttga
      661 gcgaggaaaa tgccatgaag caaacacagg aatacttacc acagccggaa ggcgctataa
      721 gcacaagcac ctctgaacag acccaggcca gcgaacgcct gctgcaagag gtcggtaagc
      781 cggaccagcc ttcaggccag ggttgccagc tcgagaagct ctgctctgtg cgctacgcca
      841 ataccgacga aatgaaaatc ccccatgcca ccgagccggt gctcaaacta ttcaaaagcg
      901 gcgacagctt ctcacaagac ggcaacagcc agcggcttac ttttgccgag ggctactcgg
      961 tggaagtagg caataaaggt cagttttcag tgcgcgatgc aagcggcaac cgcgtgcagg
     1021 cagatgaaat aatgatcaag cccggaccgg gcgttaccga gccccagaga cactatgcat
     1081 ttggagacct gatgacggca agcatatcca aagacggaca ggtcaagctg caaaccggta
     1141 caggagccgt gctagtatta gacagacgtg gtatgacttc catgagccgc gacaacaagc
     1201 aattgttcta gggctcttac tcaagggcaa gcacaagccg acagtggaaa actacttctt
     1261 agacctggcg gacaaagata ctattgagct gtccatagcg gccgagcaag tctatggtct
     1321 cgtatgccgt aacgactttt ccaagcccgg ctttgcgctg atcagacttc cggaatctac
     1381 cacctcatcc attcaacgaa tgttgatggt caatctaaaa gaccatctag cagcgctgtt
     1441 ccaaaccgaa agaggcacag ggctgagctg gttcaatatg acacgtttcg atcaaaagaa
     1501 caccactaag ttccatcggg acggcgcgcc gagcgaatcc cttctaattc tgggatacga
     1561 acccagcaag gtgcatagcg aaataagcat ggccgatttc tcccgctgcg cccatgaact
     1621 gggaatcaca ccagatcaat ttctggaaga cttcaacccc atgtacgaga aaggtttgga
     1681 aaaactagca ccgtttatta gcccagtcag caagttcgac ccggccggct ttcagatctt
     1741 agtcatcaat aacagctcca atccgctcca gccgggaaag aagagatggc agggcgtgct
     1801 acatagtgca ctggtttcaa acggcacggg cactagagtg attaactcca cctgcgtagc
     1861 cccgaccgat tcggccaaga cgagtgcagt cacagaaaaa caagtcacag aattcctcta
     1921 cgaaaacaaa cttgaaggaa gttactccct ttaagcggga gcgcttggta ttatccggcc
     1981 cagtcagagc cgaaattctg caacaacagt cccgatcagg tcaattctat agactgcttc
     2041 atctgacttc aggcggttct gctacccgat tggtggctta gcggcgccaa cctctcgcaa
     2101 tcgctctaaa acgccgaata tcatataatc gaataggagc agttgaattt cacaaaccga
     2161 ctcagcaatt attcaatatg gtgaaagacc gtgatgctac ttaagccaat ccctaataag
     2221 ctgatgaaag catttctggc ttgctctatc ctgctcttcc tccttctttc gcttatcgtt
     2281 gtgtcatgct tgagcgacac ttgttgcaac gaagacgtgg agagggcatt tcgacatgtt
     2341 gcgaacgagt taaacgaaat gaacagtgat gcgaatcccc aagattcgaa gtaatcaccc
     2401 ttggtctggc gcattaccct gagcccgacc gttgacggcg atggcgaagc tttttctcaa
     2461 atttcttcag taaatcaccc taaaaactaa aaacactcag tcttgctatt caccaatgaa
     2521 tagtcagctt caaaaagaca aagcaaaaaa aactattccc agaaaatctt ttgtgtgcta
     2581 taaaagaaaa acgtccataa ggaataaatc cgtgaaccgc gtccatcagc ataattatca
     2641 actcaacgga aaccagcata gctggtccat ttcgttgcgt cttgttggta attacgttgt
     2701 tgagtatcac ggaaatgaaa tccttaattc gaaaaatatt tgtcgaccga gaagcctcta
     2761 ataccgccat acccggggtt aggtcatcgg tagactgtct gttttgggaa cagaaggcag
     2821 aaggttcaat tccttcacct cggattgttg ctctcgtagc tcaatcggta gagcgctcgc
     2881 ctgataagcg agaggctgga ggatcatatc cttccggggg caaatttttt cgacatcgac
     2941 agcgtagcca gaactatgat ggacactacg ttccattgag atgtggtgaa actggtatca
     3001 cagcggcctt tgaagtcgcg attcctggat cgtacccagg catctcagaa ctgtggctat
     3061 cgtataaagg tattacccct ggttgtggtc caggagatga ggtttcgatt acctctagcc
     3121 accccaacat tgtgctgtag ctcagccggt agagcggctc cctgttaagg agatggtccc
     3181 tggttcgaac ccaggcagca cagccatatt acaaagaaag agtaaggaga aaaaaatagc
     3241 ttcaataatt cgaattcaag ataaaacttc ttagaaaagt atcgcaggac atggtgtcca
     3301 attcgtctcc aaaacgatag gagcaggttc gattcctgcg cgatgcgcca cttcgtccgt
     3361 gctttcatga acacggacga caccggttcg tgggtgagag gatgatacca cctgtctgta
     3421 aaacaggatc cgcaaggaca cgctcgttcg aatcgagccg aaccgaccaa atctaatttt
     3481 gttttgagct aaacttgaag gaagcaaagc aatcgaaaat attcaatcaa ttagaatcaa
     3541 agcaggcaaa gatcatatgc gcactctgca agcaaccggc ggccgaattg aaattgatga
     3601 ttcagaacca agtcactgcc agtgctttct gatcacaagc ggatcagaca agtatctact
     3661 tggctggcaa ccgctatact acctgaaaga aaagctgctg cgggccctga aagaagagcc
     3721 ggcagatcca aagaaacttt tgtttgccgg tgccttcgag ccgcttcgct tcactctgta
     3781 cttgactcgg caaggggaca caagacgtat tctcgttcag gatgatcact tacttgcctc
     3841 cggcgacgga atacttcata tcctggagct atcgcaagaa gacgtcgact catgggtctt
     3901 gacgctgcat cgctagagca atttcactgc ttatcgagtt tttctcgaat cgctttaact
     3961 cgctcagcca actcatttgc ttccgcatcc ctacccgcaa gcttcaaaag ggccgcataa
     4021 ttctcgagca aaatcgcaac ttccgggtgg tccgggccaa gcgctttttc gtaaatgagc
     4081 aaggattttt tataaagctc ttccgcttcc ttcagtttcc cctgcttctt gtatacaacg
     4141 gcaagattgt tgtaattctt tgccagactg acattatcgg ggccgaggac cttttcctcg
     4201 attgccagag actcgccgta taactgctca gcttccgtgt acttcttctg atccgcataa
     4261 agagcggcga tattagccat ggtattagcc acgctggcat gttcggcacc aaaacatttt
     4321 ttctgaatgg tgagagcctg ctgaaagatt ggctctgcct cagtatattt accttgagct
     4381 tcgtaaacca gagccaaatt attcatggcc gttgccaggt ctggatgctc ggcaccgcgc
     4441 accttctcat aaatggccag agcacgcttg tagagcggtt cggcctctac atacttgccc
     4501 tgggtttcgc acacaagggc gagattatta agggtggtag ccacccttac gtcttcggcg
     4561 ccgaatttct ccgccttggt cagggcttca gaaaaagcca gttccgctac ggcgtattta
     4621 ccctgccgat aagccataga gccaacctcc aggtcctgag accaggtggt ttcttgcgca
     4681 ccaccgggtg cggcaaaagc cagaaattgc ag
//
LOCUS       DAOQNZ010000104         4701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7770_length_4701_cov_3.978046, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000104 DAOQNZ010000000
VERSION     DAOQNZ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7770_length_4701_cov_3.978046"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKW73_02850"
     CDS             complement(<1..245)
                     /locus_tag="PKW73_02850"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72245.1"
                     /translation="MCAATKAKSKAVKETKTKTVKATKKKPAFSYYQIKITLLDSPLP
                     IWRRLVLRSDLPLSLVHEMLQIVMGWGNYHNHLFITPT"
     gene            483..1682
                     /locus_tag="PKW73_02855"
     CDS             483..1682
                     /locus_tag="PKW73_02855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72246.1"
                     /translation="MLKTCSRCGKSEEQCRCTVQTGNSGSADGFGAGAAGGLRPGAGT
                     GAGASAGAGAGTGAGAGAGVGNNAASGSVPKAGAGVSSNAAPGLSSSSSSVAKMLEFW
                     KDPASEEEDGQLQPGDLISHQYQIMRCLGSGGMSTVYQARDLTSEEVLAIKMLNSEIS
                     LDDEARKRFEREVLSMSRLRHENLLAMKGYGQTPQGHTFFVMEYLTGRSLATELIRKG
                     PLEPRRACGIFLQVCAGMAHVHEAGIIHRDLKPGNIYLVGQDGVDRVKVLDFGIAKME
                     VNLEGHNNTLTQVGQVLGSPRYMSPEQCTGMKLDSRSDIYSLGCVMYQALTGQTPFNG
                     ENALNILFKQVNERPPAFSVVTAQPLIPPVLEAIIMRALAKSPNDRHQTMSALAEELQ
                     RFLDSSN"
     gene            complement(1709..2362)
                     /locus_tag="PKW73_02860"
     CDS             complement(1709..2362)
                     /locus_tag="PKW73_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNA72247.1"
                     /translation="MDLVLKPDNTPSNYPGKHTSKRENQVKSVAVFCGASMGNSEEIK
                     NAAQQMGELIAKAGLTLVYGGGRVGLMGVVADAALSQGGTVVGVIPKFMSDREVAHSG
                     LSELIIVETMHERKAIMANRSDAFLAMPGGYGTLDELCEILTWYQIGLHKRPIGILNT
                     MGYFDSLLGFFDDAVKNGFIPANLRAILLESSTPEQLLKQLADHHRESLNHESFARG"
     gene            2487..3242
                     /locus_tag="PKW73_02865"
     CDS             2487..3242
                     /locus_tag="PKW73_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72248.1"
                     /translation="MAELGTAIMQYNSKVNIADLCIARQVELFDHLMDEAERSWPKRL
                     RSQKQLEESCHSLVMRPLSCDILQTAMVPLREQFPAESNCGLIYYEPCSMHVTVFGLP
                     GFVVGGALHRHLERLLMAQTPALRPFTTVVSGLSVIGDALVARCLDKDGKIVRFVNAC
                     LDELSEELGDEVLELVRGAELHRSIFWVTLARLRSDLDTDRGRLLLQEVSKRRHLELG
                     VLSFDAVSLSLTNGLFSIDKTTDLSRFELGLLT"
     gene            3339..4085
                     /locus_tag="PKW73_02870"
     CDS             3339..4085
                     /locus_tag="PKW73_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNA72249.1"
                     /translation="MLVRPQFLGNIGGTARVMKNFGFKQLRFVRAPREYKDAEARKMA
                     VDAIDVLKGAQLFDSLEEALADIGAAFGTTSCQNRQVKTTPIGSMKERIVQLASSNKV
                     ALVFGDERDGLFNAELSLCHELLTIPTNPQFPSLNLAQAVGVCAYELERTVRSEEVDL
                     KLRTTADCQKSPPTLASGADNDELFQHLAAVLHDAGFTRSYNHERVMMELRSFYLRAY
                     PTERETALLRSAIRKIAAAVKTEMPGEGDS"
     gene            4088..4351
                     /locus_tag="PKW73_02875"
     CDS             4088..4351
                     /locus_tag="PKW73_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72250.1"
                     /translation="MLQKRAGRATAWCLLLMVVLCLSGAPAVAGDLKAASAATEKQRL
                     AQYNQLLDQADNERKLQNLPASLALYDRAEKLFPKDTSTYESA"
     gene            4338..4541
                     /locus_tag="PKW73_02880"
     CDS             4338..4541
                     /locus_tag="PKW73_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72251.1"
                     /translation="MNLHDAGQMYLLANVTSEAMACFELLIKTNPQLSTGYWDRAQVY
                     DLKKEKTKAAADRAKARKLDLDY"
BASE COUNT         1086 a         1090 c         1346 g         1179 t
ORIGIN      
        1 gtcggtgtga tgaaaagatg gttgtgatag ttgccccaac ccatcactat ttgcaacatc
       61 tcgtgcacca gcgacaaagg caggtcggag cgcaggacca agcggcgcca gataggcagt
      121 ggactatcaa ggagggtgat ctttatctgg tagtaagaga aggcaggttt tttcttagtt
      181 gcttttactg tcttggtttt ggtttctttg acagctttgc ttttggcttt ggtggcggcg
      241 cacatctttt atttagtcct caaaaactca attatagagc tcttgacgcc ctggttgccg
      301 cttatgaggc ggaatgctct atgtaaatcc cttgaattag gctaaactga ccacctaact
      361 agattctctt tgaaaaagca gcaagtgaaa attctgatct taaatattct cagtgtacgt
      421 tttggttttt gcaaaacctg gggatgctgg aatcggagcg ggcgctgcgg gaggcggaga
      481 aaatgttaaa gacttgctcc cgctgcggta aatcggaaga gcagtgccgt tgtaccgtgc
      541 agactggaaa ctccggttct gccgatggtt ttggcgccgg tgcagcgggc ggtctcagac
      601 ctggcgccgg aaccggtgcg ggcgcgagtg cgggtgcagg agcaggaaca ggcgcgggtg
      661 caggtgcggg cgtgggtaac aatgcggctt ccggctcggt tcccaaggcc ggtgcgggcg
      721 tcagctcaaa tgccgccccc gggctcagct caagttccag ctcggtagcc aagatgttgg
      781 agttttggaa agacccggct tccgaggagg aagacgggca gttgcagccg ggcgatttga
      841 tttcccacca gtatcaaatt atgcgctgtc ttggttcagg cggcatgtcc acggtttatc
      901 aagcgagaga tttgacctcg gaagaggtgc tggctataaa aatgctcaat agcgagatct
      961 cgcttgatga cgaagcccgc aagcgtttcg agcgggaagt tttgtccatg agccgtctca
     1021 ggcatgaaaa cttgttggct atgaaaggtt atggtcagac gccgcaggga cacacatttt
     1081 ttgtcatgga gtacctgacc ggccgcagcc tggctaccga gctgattcga aaaggccctc
     1141 tggagccgag gcgggcctgt ggaattttcc tacaggtctg cgccggtatg gctcacgtgc
     1201 atgaggcagg cattattcac agagacctga agccgggcaa tatttacctt gtggggcagg
     1261 acggcgtaga tcgcgtgaag gtgctcgatt tcggtattgc caaaatggaa gtgaatctgg
     1321 aagggcacaa caataccctt actcaggtgg gacaggtgtt gggcagtcct cgctatatga
     1381 gcccggaaca gtgcactggt atgaagcttg acagtcgttc cgatatttac agtctgggct
     1441 gcgttatgta tcaagccctg accgggcaaa cacccttcaa tggtgaaaac gcgctcaata
     1501 tactattcaa gcaagtcaat gaaagaccgc ctgccttttc tgttgtgaca gcccagccct
     1561 tgattccacc tgtgcttgag gcaatcataa tgagggccct ggctaagtcg ccaaatgata
     1621 ggcatcaaac aatgtctgct ctggctgaag aactacagcg ttttctggat agcagtaatt
     1681 agaataaata gcgacttggg ccggttattt agccgcgggc aaagctttcg tggttcaggc
     1741 tttcccggtg atgatcagcg agttgcttga gaagctgctc aggggtggaa gactcaagca
     1801 gaatcgctct cagattggcg ggaataaagc cgtttttgac ggcatcgtcg aaaaatccga
     1861 gcagggagtc aaaatagccc atggtattga gtatgcctat gggtcttttg tgtaagccaa
     1921 tttggtacca ggtgaggatt tcgcaaagct catcgagggt gccgtaaccg cccggcatgg
     1981 ctaaaaatgc atcggagcgg ttggccatta tggcttttcg ttcatgcatg gtctctacaa
     2041 taatcagttc ggacaggccg ctatgggcaa cttcccggtc gctcatgaat ttggggatga
     2101 caccaactac ggtgccaccc tggctgaggg cagcgtcggc taccacaccc atcagcccta
     2161 cccgaccgcc accgtaaacc agagtcagac cagctttggc aataagttct cccatttgtt
     2221 gggcagcgtt cttaatctct tcgctgttgc ccatgctggc gccgcaaaat actgctactg
     2281 atttcacctg gttttccctt ttgcttgtgt gcttgcccgg gtagttactt ggagtattgt
     2341 ccggtttgag gactaaatcc aatcaggagc ttccgaagcc tgcctgtaat gtccatttta
     2401 tctaatactt caagggctgt gcgaagggct gtgggcggtg aggcacaact aatgagaggc
     2461 aagcctctga agtggatgtc atatggatgg ctgagttggg aacggcgatc atgcaataca
     2521 atagcaaggt gaatatcgca gacttatgta tcgccaggca agttgagctc ttcgaccatt
     2581 taatggacga ggccgaacgg tcctggccca agcgactgcg cagtcagaaa cagttggaag
     2641 agtcctgcca ctcccttgtc atgcgtcccc tttcttgtga tattttgcaa acagccatgg
     2701 ttcctttgag ggaacagttt ccagctgaga gtaattgcgg tctgatttat tacgaaccct
     2761 gctcaatgca tgtgaccgta ttcggtctgc ccggctttgt ggtaggcggc gcattgcacc
     2821 ggcacctgga gcggctttta atggcccaga cgccggcttt gcggcccttt accaccgtgg
     2881 tctccggtct cagtgtaatt ggtgatgccc tggtggcgcg ttgtctcgat aaagacggca
     2941 aaatagttcg ctttgtcaat gcctgcctgg atgagctttc ggaagaactc ggcgatgagg
     3001 tgctggagct ggtgcgtggt gccgaacttc atcgttctat tttctgggtt accctggctc
     3061 gcttgagatc cgatctggac actgatagag gcagattgct tctgcaagaa gtaagcaaac
     3121 gccgacatct agaattgggt gttctttctt ttgatgccgt cagcttgagc cttaccaacg
     3181 gtttgttttc catagacaaa acgaccgact tgtccaggtt tgagctgggg cttctgacct
     3241 gagactttgg gggggggacg atcataaacg aaagtatctc cgattcagat tgcgtttcaa
     3301 acgagggtaa ctgtctcaac aaggacaacc tggtcttcat tctggtgcgt cctcagtttc
     3361 tcggtaatat cggcggcacc gccagggtga tgaagaattt cggcttcaaa caattgcgtt
     3421 ttgtgagggc accgcgcgaa tacaaagacg ccgaggcgag aaagatggct gtggatgcca
     3481 ttgatgtctt gaaaggggcc cagttgttcg attctctgga agaagctctc gctgatatag
     3541 gcgctgcttt cggtacgact tcctgtcaga accgacaggt gaaaaccacc cctataggtt
     3601 ctatgaaaga gagaatagtg cagctggcaa gcagcaataa agtggcactg gtctttggcg
     3661 atgaacggga tggcttattc aatgcagaac tatccctctg tcacgaactg ctgaccattc
     3721 cgaccaatcc tcaattcccc tcgctcaatt tggcgcaggc cgtgggggtt tgcgcttatg
     3781 aactggagag aacagtgcgg tcggaggagg tcgatttgaa gctccgcacc acggccgact
     3841 gccagaaaag cccgccgact ctggcctcgg gtgccgacaa tgatgagctt ttccagcatc
     3901 tggcggcagt tttgcatgat gccggtttta ctcgttctta caatcatgaa cgagtgatga
     3961 tggaattgcg aagcttctat ttgagagctt atccgaccga gagggagact gccttgctgc
     4021 ggtctgccat acgcaagatt gccgctgccg ttaaaacaga aatgcctgga gagggcgact
     4081 cttgaagatg ttgcaaaaga gagctggaag ggctacggcg tggtgtcttc tcttgatggt
     4141 ggtgctttgc ctctctggtg caccggctgt ggctggcgat ctgaaagctg cgagcgctgc
     4201 tacggaaaag cagaggctgg ctcagtacaa tcaattgttg gaccaggccg acaatgagcg
     4261 aaagttgcag aatctgcctg ccagtctggc tttgtacgat cgtgccgaaa agctatttcc
     4321 caaggacacg agtacatatg aatctgcatg acgccgggca gatgtatctt ttggcgaatg
     4381 ttacatctga ggccatggct tgttttgagt tgttgatcaa aaccaatcct cagttatcca
     4441 ccggatattg ggacagggcg caggtttacg acctgaaaaa ggaaaagaca aaggcggcgg
     4501 cagacagggc gaaagccagg aagcttgact tagactatta gagaagcttg ttttaggtta
     4561 tttgagaatc tcgttttggg ctattagaaa agtaagaact ggtgccggaa gttagcggtc
     4621 gggtagctct tttaaaaatc tgccggaatg taacagcgat tccgcagagt ggctgttgaa
     4681 ccgggcttag acgcagtggc a
//
LOCUS       DAOQNZ010000105         4691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7806_length_4691_cov_4.546160, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000105 DAOQNZ010000000
VERSION     DAOQNZ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7806_length_4691_cov_4.546160"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PKW73_02885"
     CDS             complement(<1..935)
                     /locus_tag="PKW73_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA72252.1"
                     /translation="MSQWKRIVGIFMLALMATGSVSLPASGHRTQPVDVYHRAWQLVR
                     DNYYDSNFNGKNWNELEHKFDSQIKTTADAHKYVKVMLESLSDPYTRFLDPRAFQDEN
                     DAIDARIVGIGINLMQSKDQQKLIINRVIEGGPAERNGVASGDEIAAIDSYNATGMTP
                     EQAADRIRGKAGTPVQLTLRHGASLKTVNITREEITIKAVNSHLDKNTNIGYITLTTF
                     ISNDAAEEFKQALQKLNRADGLIIDLRDNPGGLLSNALEIADMLLEGGAIVSTISRHG
                     RNTDIASGAPVSHQPIVILVDNESASASEILASAL"
     gene            complement(1182..2744)
                     /locus_tag="PKW73_02890"
     CDS             complement(1182..2744)
                     /locus_tag="PKW73_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNA72253.1"
                     /translation="MAYTDLRDFIDVLSRQGELMRITTEVSADLELAEITDRVSKLKE
                     GNKALLFTNVKGYDMPVLINAFGSHRRMCLSLEVDNLDSIADRIRSFIKPKVPESFME
                     KLSFLPMLMEVAKFPPKVTGGHAPCQEVVITDPNSPMLDKLPILKCWPEDGGPFITMG
                     AIITKDPRHGNRNVGMYRLQKFDNVTTGMHWHKHHDGARNFEESRRQSMATAMEAEPS
                     GAVQPPEPPNYGTFFDKEAYEKPGNRLEVAVVLGADPAVTYSATAPLPPEIDELIFAG
                     FLRQSPVRLTRCKTIDLEVPANAEIVIEGYVDQSDLRREGPFGDHTGFYSLAGLFPVF
                     HVTAVTHRKDPIYQTTIVGKPPQEDCFLGKATERIFMPMVQMLVPEIVDMNLPWEGVF
                     HNCVIVAIDKRFPGHAKKVMSALWGLGQLMFTKFAVIVDKEVNVHDLSEVALHVFGNT
                     DPRRDMMFADGPLDILDHASPLMGYGSKVGIDATKKWASEGFTRDWPAPIVMSKEVRE
                     HVSKQWSKYGFK"
     gene            3298..4536
                     /locus_tag="PKW73_02895"
     CDS             3298..4536
                     /locus_tag="PKW73_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72254.1"
                     /translation="MKRAIQKLEDYWKSAEKAQTEGKYGYAESIWYAALEEAQELPRN
                     DRRRALVMERLCECLWFQKKFDDAVPLAKELVGIYGEVLGHEHFDTACMVANLALLYH
                     VLNDYNNAEPLLSRAHQIKLRELGAQHPEVVHLYNTLVEVRRALGLDKQEKSTVSSLI
                     SGRQWSKTGRFQAVKLPEAPTPPKMTEVDVSASWKKHFDDAKFQADQCQWAKAEVELT
                     QALSLIEQNSITDARRWTTLSALVDVLSKQDKHLQAANHAVRLHEILSLKEGGITQVT
                     ADSLNSLAKLYYYGGDIESALRYAAVCTKQYEHIFGAEDPSVATCLVNLGLLQHLGKH
                     YLDAENSYKAALNIRTKKLGAEHPLTTQVLKNYANLLKETHRDEEAEHMQTCATGFVT
                     GTWNVVELEERLTVESEPVI"
BASE COUNT         1020 a         1135 c         1335 g         1201 t
ORIGIN      
        1 ttgagagcac tggccagaat ctcggaggcg ctggcactct cgttatcgac caggatgacg
       61 atgggttgat gggaaaccgg tgcaccggaa gcgatatcgg tgttgcgtcc gtggcggctg
      121 atggtgctga cgatggcgcc tccttccagg agcatgtcgg cgatttccag ggcattggag
      181 aggagaccgc ccgggttgtc tctcaggtcg atgatcagac catcggcgcg gttgagtttc
      241 tgcagggctt gcttgaattc ttccgctgcg tcattggaga tgaaggtggt gagggtgatg
      301 tagccgatat tggtattttt gtcgagatgg gagttcacgg ctttaatggt tatttcttcc
      361 ctggtgatgt tgacggtttt gaggctggct ccgtgtctca gggtcaattg caccggtgta
      421 ccggctttac cgcggatacg gtcggcagcc tgttcgggag tcatgcccgt ggcattgtag
      481 gagtcgatgg cggctatctc atcgccggat gcgacgccgt ttctctctgc cggtccgcct
      541 tcgatgacgc ggttgattat aagcttttgc tgatctttgc tctgcatcag gttgatgcct
      601 atgccgacga tgcgagcatc gatggcatca ttctcgtcct ggaaggccct ggggtctaga
      661 aaacgtgtgt aaggatcgct gagtgactcg agcatcactt ttacatattt atgggcatcg
      721 gctgtggttt tgatctggct gtcgaacttg tgctccagtt cgttccagtt tttgccgttg
      781 aaattggaat cgtagtagtt gtctctaacc aactgccagg cccggtgata aacatccacc
      841 ggctgggtgc ggtgtccgga agccgggagg ctgacggagc ctgtcgccat caaggcgagc
      901 atgaaaatac caactatgcg cttccactgg ctcatagaat acaccgaaga gaatatgtga
      961 tgaatcgagt tctttagctg ctttcagctc tttgttctta cccttgggta gaagcggaag
     1021 cctcggcaat cgtatgaatt cgtatttctg atattcccgt ctattcgggt ttttcaacac
     1081 tgttagatta aatagtggct cttggcaacc gttcgggcag gaatccttca aaaatttccc
     1141 ctgcggtttg cttgcagagc cgttttagtg ctcagagctt tttatttgaa gccgtacttg
     1201 gaccactgtt tggagacatg ctcccgtacc tctttagaca tgactattgg cgccggccag
     1261 tcccgggtga aaccttccga ggcccatttc tttgtggcat cgatgcctac ctttgagcca
     1321 tagcccatga ggggcgaggc gtggtccagg atgtccaggg gaccatcggc aaacatcatg
     1381 tcccggcgcg ggtcggtgtt gccgaagaca tgcagggcca cttccgacag gtcatgcaca
     1441 ttcacttcct tatcgacaat gacggcaaac tttgtgaaca tcagttgccc caggccccac
     1501 agggcactca tcaccttttt ggcgtgccct ggaaaacgtt tgtcgatggc cacaattaca
     1561 cagttgtgga aaacgccctc ccagggcaga ttcatatcaa ctatctcagg tactagcatt
     1621 tgcaccatgg gcatgaagat gcgttcggtg gctttgccga ggaaacagtc ttcctggggc
     1681 ggctttccta caattgtggt ctggtagatg ggatctttgc ggtgagtgac ggcggtcacg
     1741 tggaaaaccg gaaagagtcc ggccaggcta taaaagccgg tgtgatcgcc gaagggacct
     1801 tcccttctca ggtcgctctg gtccacatag ccttcaatga caatttcagc gttagccggt
     1861 acttccagat cgatggtttt gcacctggtg aggcgcactg ggctctgtct gagaaaaccg
     1921 gcaaatataa gttcgtctat ctccgggggc aggggcgccg tagcactgta ggtgacggcc
     1981 ggatcagcgc ccagcaccac tgctacttcc aggcgattgc ccggtttctc gtaagcttct
     2041 ttgtcgaaga aagtgccgta gttgggcggc tccggcggct gtacggcgcc gctcggctcg
     2101 gcttccatag cagtggccat ggattgtctg cggctttcct caaaattacg ggcgccgtca
     2161 tggtgcttat gccagtgcat accggtggtg acattgtcga acttttgcag gcggtacatg
     2221 cccacgttgc ggtttccatg gcgtggatct ttggttatta tggcgcccat ggttatgaag
     2281 ggaccgccgt cctcgggcca gcatttgagg atgggaagtt tgtccagcat gggtgagtta
     2341 ggatcggtta tcaccacttc ctggcagggg gcgtgtccac ctgtcacctt gggggggaac
     2401 tttgccactt ccatgagcat gggcagaaag gagagcttct ccataaagct ctccggtacc
     2461 ttcggcttga taaatgagcg gattcgatcg gcaatggagt ccaaattgtc cacttccagg
     2521 gagaggcaca tgcgcctgtg ggaaccgaag gcgttgatca ggacgggcat gtcatagcct
     2581 ttcacattgg tgaagagcag ggccttgttt ccttccttca gcttgctgac gcggtcggtt
     2641 atttcagcca gttccaggtc agccgagact tccgtagtga tgcgcattag ttcgccctga
     2701 cggctcaaaa cgtctatgaa atctctcagg tcggtgtatg ccattgatca aataccgctt
     2761 tcatctttgc ttttcccgag accacggata tctaccggat acctgccgga tacttatagg
     2821 ttcggagaaa agggggtgtc gttagatatt attggcacat tgccggaagt cgttcacgat
     2881 tgggaacgca aatttatata tagatattta gagatagccg ggggctgggt ataaaatcct
     2941 tgcctcctcg ggggcatgga tttgttaata tcccggattc ggaattttgt tgctccagac
     3001 aatatgggct tgccgtttgt ctggacttga gggtggtcac atgtcaggca gaaaactcac
     3061 ctgtgtactt tgtcttctgg ctgctgcctg ctttgggttt caaattctcg tacaaagctt
     3121 tgttaaatcg gtttagacct ctctttgagg cggtatctag tattgcgagc aattttgaaa
     3181 gctgattttt gatctgataa tgaaaatagg aatccctgaa tttcccctgt cgaggtacct
     3241 gtggttaata gagatgcaaa caagtcttag catctgcgga tccgggtatt tgaaaagttg
     3301 aaaagagcaa tacagaaact ggaagattac tggaagtcag cggagaaagc ccagaccgaa
     3361 ggtaagtatg gttatgccga gagcatatgg tatgcggccc tggaggaagc acaggagttg
     3421 ccgcgtaatg acagacgccg tgctctggtt atggaacggc tttgtgagtg cctctggttt
     3481 cagaagaagt ttgatgatgc cgtgccgctg gccaaggaac tggtgggcat ctatggcgaa
     3541 gtgctggggc atgagcattt cgataccgcc tgtatggtag ccaatttagc cctgctttat
     3601 catgttttga acgactacaa taatgccgag ccgctcctca gccgggctca tcagataaaa
     3661 ctgcgagaac tgggtgccca gcatcccgag gtcgtgcatc tctacaacac cctggtggag
     3721 gtgaggcggg ccctgggtct ggataagcag gagaagagca cggtttctag tttgattagc
     3781 gggcgccagt ggagcaagac gggacgcttt caggctgtga aattgccgga ggctccgact
     3841 ccgcctaaaa tgacggaagt agacgtttcc gccagttgga agaagcattt tgacgatgcc
     3901 aagtttcaag ccgatcaatg ccagtgggca aaggcggaag tggaattgac ccaggcgctt
     3961 tcgcttattg aacaaaacag tatcaccgac gccaggcgct ggacaacact ttccgctctg
     4021 gttgatgtgc tctccaaaca agacaagcac ttgcaggcag ctaatcatgc tgtcaggctg
     4081 catgagattc tctcgcttaa agagggtgga atcacccagg tcactgccga cagcctcaat
     4141 agtttggcca agctctatta ctatggcggc gacatcgaga gtgcgctgcg ctatgctgct
     4201 gtttgtacca aacagtacga gcatattttc ggagcggaag acccttcggt tgccacttgc
     4261 cttgtgaacc tgggtttgct gcagcacctg ggcaagcatt atctggatgc cgagaattca
     4321 tacaaggccg cgctcaatat aagaaccaaa aaattgggtg ccgaacatcc gctcaccacc
     4381 caggtattga aaaactacgc caacctgctc aaagaaaccc atcgcgatga agaggcggag
     4441 cacatgcaaa cctgtgccac cggctttgtc accggcacct ggaacgtcgt ggaactggaa
     4501 gagcgcctca ctgtcgagtc tgagccggtt atctaggctc aattaatact tggaagagtc
     4561 ttcataggct ttggagctga ggcagtcctt caaggctttg ctcagcaccg ccgccggtgc
     4621 gtcggccggc actgccattt ccggagccag aagccagcaa tttttaggta gaaaaagaac
     4681 tgtcggggtc t
//
LOCUS       DAOQNZ010000106         4678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7853_length_4678_cov_3.899632, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000106 DAOQNZ010000000
VERSION     DAOQNZ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7853_length_4678_cov_3.899632"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PKW73_02900"
     CDS             complement(<1..1166)
                     /locus_tag="PKW73_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA72255.1"
                     /translation="MVLEQLHSDRITERNLAAVFFTRAKGLGEHPAVKYKDKKEPYKT
                     MSWKDYSRLVREIALGLMTLGLKPDEKAAIFSSTTHYWVAADFATISNGAVSVPIYHT
                     SSAFDIEYIIENSQARVVFVQNETLLKKLAAVREKLTCLHKIVVFYLPEDAKTAEELA
                     RKHNLPTQLLTDIESLRKLGSSLDTAHLKILEDRMKSTTLDSVATIIYTSGTTGVPKG
                     VPLTHGNIISVLEDIKPVLPIDEQDVYLSYLPLSHVFERICGEFYWMVKGGTCAFAES
                     IESMGRNMAEVEPSMILVVPRVLDRIYSKVKAGIEGASGRKKQIIDWALSVGEEMVDR
                     QSEKKAVGPILLLKHVIADKLALSKLREKIGPHLRLVVSGGAPATRETLRFFNS"
     gene            complement(1327..2235)
                     /locus_tag="PKW73_02905"
     CDS             complement(1327..2235)
                     /locus_tag="PKW73_02905"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA72256.1"
                     /translation="MPSDINLTPTGSLIEIVPQSHGSTQGTSQAIPGRTWGTASECRA
                     AALLVHGLGAHSGWYEALGRRLRVKRFFSLAYDQVGFGKRRNQTLMMKQQWLDDIKEA
                     YDHLRNTVGDKPIFVMGNSMGALLALKASGKLGQAGSEAPAGLVLFSPGFDGHPQTFK
                     VSYRAKAIYKALFDPDAELALPYTFDDITREETVRNWLANDPDQRTVLPARMLLELLK
                     LSQEVKTSVKKVNCPVLMLTAGQEKIVDNQVNEQVFERLSAPKKTSHRFEAAWHDLMF
                     DPLLDEVVEMVSAWMIEVAKERQAVG"
     gene            2304..3827
                     /locus_tag="PKW73_02910"
     CDS             2304..3827
                     /locus_tag="PKW73_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72257.1"
                     /translation="MTESGSEFEKVSVPGSGKLKPLLVFLVVALVAGLSGYIVFTPRE
                     TPLGKAVKLLNSGRAAQAVPLLEDLYKQNPADGAVYPWLAQGYLATDRVAEGRTALDT
                     AFRVGVKDESLASVVDNFSVYYQNRGHFEEAERLCHSAQNSVNADKLSGSLARMYFKW
                     SEADMAAGNLDGAVERLNLTRRYAGYLEEPLKAQVPHLLARCYRELSALAQTRDKDDQ
                     KAIELLEKSVAACDEPMSRMALGGIYTKLGDKRKAVENYQVVAQSDPNNLEVRHRLVE
                     LFIELKEIKGAQAALTELVDKERSFENYELLAGLNLKLANYAGAVRALEEACALRPTA
                     ALLKQLISTLDKWSGQLVSENKAQEALSVKGHAERVSEKLEALLKEEKKNEPKVEVKP
                     DKWNPGSPPISIISSRNWLARGSLTPEGEIKIKNISGAPVEDLTLTAVFYDNTRRRSK
                     GSVVLPVASSAANAFAPGDERTLYFSCPNIVPADHHLAVMILWKGKFLKEFPVIKQP"
     gene            3841..4359
                     /locus_tag="PKW73_02915"
     CDS             3841..4359
                     /locus_tag="PKW73_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72258.1"
                     /translation="MEEPSNSNSSFQEASTPEVLPPKPACRHEYVRIFQRRLKGLDRR
                     LVSTRAYLPTGFKMGDFSHLSEGSFCFCVSCRARLFPKRTQAEKAQARLLAAQGKALS
                     QESQDLLDEVGADVLSEVLAESEQIEAVRESRDINVDELVPESVDVQDIEAEGIKLTD
                     EEESCELAEEEN"
     gene            4388..4510
                     /locus_tag="PKW73_02920"
     CDS             4388..4510
                     /locus_tag="PKW73_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72259.1"
                     /translation="MGLLDKLMLLSVIIPPGYIIFRFCRWYVESVQHEDEEFID"
BASE COUNT         1080 a         1152 c         1323 g         1123 t
ORIGIN      
        1 attgaattga agaaacgcag ggtttccctg gtagccggcg cgccgcctga aaccaccagt
       61 ctcaaatggg gaccaatttt ttcccgcagt ttggaaaggg cgagcttatc ggcaatgacg
      121 tgcttgagca gaagaatggg acccacagcc ttcttctccg actgccggtc gaccatctcc
      181 tcgcctacgg agagagccca gtcgatgatt tgcttcttgc ggccggaggc gccttcgatg
      241 ccggctttca ctttggaata gatgcgatcg agaaccctgg gaaccaccag gatcatgctg
      301 ggttctacct cagccatatt gcgacccatg ctttcaatgc tctcggcaaa ggcacaagtg
      361 ccgcccttca ccatccagta gaactcgccg cagatgcgtt caaatacatg ggagagcggt
      421 aaataggaga gatagacatc ttgttcgtcg atgggcagaa caggcttgat gtcttccaga
      481 acagagatga tgttaccgtg ggtgaggggc acgcctttgg gaacaccagt ggtgccggac
      541 gtgtaaatga tggtggcgac gctgtccagg gtggttgatt tcatcctgtc ttccaggatt
      601 ttcaagtgag ccgtatcgag actgctgcct agtttgcgca agctttcaat gtcggtgaga
      661 agctgagtgg ggagattatg tttcctggcc agttcttctg cagttttggc atcttccggc
      721 aggtaaaaga ccactatctt gtgcaggcaa gtaagctttt cgcgcaccgc cgccaatttc
      781 ttcaacaggg tttcattctg aacaaagacg accctggcct gcgagttctc aatgatgtat
      841 tcaatatcga aagccgatga ggtgtgataa atcggcacgg aaacggcgcc attggatatg
      901 gtggcaaagt cggctgccac ccagtaatgg gtggtagaag aaaagatggc tgctttttcg
      961 tcgggcttca agcccagggt catcaaaccg agcgcaattt ccctgaccag gcggctgtaa
     1021 tctttccagc tcatggtttt atagggttct ttcttgtctt tgtatttgac ggcagggtgc
     1081 tctcccaggc ctttggcgcg ggtgaaaaat actgctgcca gattacgttc ggttatgcgg
     1141 tcggaatgaa gttgttccaa aaccatctag aaatccctct aatacacgcc gagccttatt
     1201 atattcagtt ttcagtatga gtcgagaagc ctaaattatt tctcggaatt gccgtatcgc
     1261 cagtgtatat gcgcagcatt gccgctctcg gtgagaggaa tataaactga ccttggcacc
     1321 ggaaatttag ccgacggcct gtctttcttt ggctacttca atcatccagg cggagaccat
     1381 ttccaccact tcgtccagta gcgggtcgaa catcaaatcg tgccaggccg cttcgaagcg
     1441 atggctggtt ttcttaggtg ccgagagcct ctcgaagacc tgctcgttta cttgattgtc
     1501 gactatcttc tcctgaccgg ccgtgagcat gagcaccggg caattgacct ttttcacgga
     1561 agtttttact tcctgactga gtttgagcaa ttctagaagc atgcgggcgg gcagcaccgt
     1621 gcgctggtcc gggtcattgg ccagccagtt gcgcacggtt tcctcacggg tgatgtcgtc
     1681 aaacgtataa ggaagggcca gttcggcgtc gggatcaaaa agggctttgt agatggcctt
     1741 tgcccggtag gaaaccttga aggtctgggg gtggccgtcg aagcctggcg aaaagaggac
     1801 cagcccagcc ggcgcctcac tgcccgcctg tcccagcttg ccggaagctt tcaaggccag
     1861 aagggcaccc atggagttgc ccatcacaaa tataggtttg tctccgaccg tatttctcaa
     1921 gtggtcatag gcttctttga tgtcgtccag ccattgctgc ttcatcatca aggtctggtt
     1981 gcgccttttt ccaaagccta cctggtcata agccagtgag aagaagcgct tcaccctcag
     2041 gcggcgtccc agggcttcat accatccgga atgggccccc agtccgtgaa cgagcagggc
     2101 ggcggcgcga cactccgaag cagtacccca ggtgcgaccg ggaattgctt gactagtgcc
     2161 ttgagtcgaa ccgtgagatt gtgggactat ctcaatcagc gacccagttg gtgtcaggtt
     2221 aatatcagat ggcataagtg ggagctatta tagcttctga taccactttg catacagcac
     2281 agaccggcag aaatgaaata gaaatgacag aatcgggctc tgaatttgaa aaggtatcgg
     2341 tgcctggcag cggtaagctg aagccgttat tggtctttct ggtagtggct ctggttgcag
     2401 gcctttcagg ttacattgtc tttactcccc gtgagactcc cctgggtaaa gcggtcaagt
     2461 tgctgaacag tggaagggcc gctcaggctg taccgcttct agaagacctt tacaagcaga
     2521 atccggctga cggagccgtc tatccctggc tggctcaggg ttatctcgcc accgacaggg
     2581 tggcggaggg ccgcacagcc ctggacactg cttttcgcgt gggggtgaag gatgagtctc
     2641 tggcttccgt ggtggataac ttttctgttt actaccagaa tcgcggtcat tttgaagaag
     2701 cggagcggct ttgccattcg gcccagaatt cggtaaatgc cgacaagctt tccggaagtc
     2761 tggcccgcat gtatttcaag tggtcggaag ccgatatggc agccggcaat ctcgatggtg
     2821 cagtggaacg attgaatctg accaggcgct acgccggcta cctggaggag cccttgaaag
     2881 cacaggtgcc gcaccttctg gcccgttgtt atcgagaact aagtgccctg gctcagaccc
     2941 gggacaagga cgatcaaaaa gccattgagc tgcttgaaaa gagtgtggct gcttgcgatg
     3001 agccgatgtc gcgcatggcc ctgggtggaa tttacaccaa acttggtgat aagcgtaaag
     3061 ctgtagaaaa ctaccaggtc gtggcccaga gtgatccaaa taacctggaa gtgcgccatc
     3121 gactggtgga gctcttcatc gaattgaagg agatcaaggg tgctcaggcc gccctgaccg
     3181 aacttgtaga caaagagaga agttttgaaa actatgagct tctggccggt ctgaatctca
     3241 agcttgccaa ctatgccgga gcggtgagag ccctggaaga agcttgtgcc ctgagaccga
     3301 ccgccgctct tctcaagcag ctaattagca ctctggacaa atggagtgga cagttggtga
     3361 gcgagaataa ggcccaggaa gcgctctccg ttaaggggca tgctgagagg gtgagcgaga
     3421 aactggaagc tctcctgaaa gaggagaaga agaatgaacc caaggtagag gtcaagcccg
     3481 ataaatggaa cccaggctca ccgcccattt ccataatttc ttcacgcaac tggctggccc
     3541 ggggcagtct cactcccgaa ggggaaatta agatcaagaa tatctccggc gcgccggtag
     3601 aagacctcac cttgactgct gttttctatg acaacaccag acggcgctcc aaggggtccg
     3661 tggtcttgcc ggtggccagc tccgccgcca acgcctttgc tcccggcgac gagcgcactc
     3721 tttatttttc ttgtcccaac atagtaccgg cggatcacca tctggcggtt atgattctat
     3781 ggaagggcaa attcctcaaa gaatttcccg taattaagca accttgagtg attaatcagt
     3841 atggaagagc caagcaactc caattccagt ttccaggaag cttcgacgcc cgaagtactg
     3901 ccgcccaaac cggcctgtcg tcatgagtac gtgcgcatat tccagcggcg tttgaagggg
     3961 ctcgatagaa gattggtctc tactcgggct tatctgccta cgggcttcaa gatgggagat
     4021 ttctcccatt tgagcgaagg cagcttttgt ttttgcgttt cctgtcgggc gcgccttttc
     4081 cccaagcgca cccaggctga aaaggcgcaa gctcgccttt tggcggccca gggcaaggcc
     4141 ttgagccagg aaagccagga cttactcgat gaagtcgggg ctgatgtctt gagcgaagtg
     4201 cttgctgaga gtgagcagat agaagcggtg agagaaagta gagatataaa tgtagacgag
     4261 cttgtgcctg agtcggtgga tgttcaggat attgaagccg aaggtattaa gttgaccgat
     4321 gaggaggaga gctgtgagct tgctgaagag gaaaactaaa aagagaagcg gaaagtccag
     4381 gggcttgatg ggcctgcttg ataaattgat gctcttgagt gtaataattc cgcccggtta
     4441 catcattttt cgtttttgtc gctggtacgt ggaaagtgtc cagcatgaag acgaggaatt
     4501 tatagactag atgcttgatt atctactgag agtgatgcgg gccatgctcg tcacttccct
     4561 gggcgtaggg ggtgggatcg ggctgctagt tctgattgta gttctcacta ccaagaatga
     4621 gcccaacgcc ttcaaatacg gtatcactgt cggtctgctc ctgggtggga tgtttgcc
//
LOCUS       DAOQNZ010000107         4676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7862_length_4676_cov_4.266825, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000107 DAOQNZ010000000
VERSION     DAOQNZ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7862_length_4676_cov_4.266825"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PKW73_02925"
     CDS             <1..1030
                     /locus_tag="PKW73_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72260.1"
                     /translation="HSDLLNRSKAYRAKGMLKEAARDAALAEKYALLEKLQAFRIQFK
                     LKEAKQTGDEAVKQLPESSYANLLGGVSALNFASYREAFDRFSKVISKHPDCSFAYYF
                     RADAARGLKKFAQAQSDYDKILSLKPRYVAMSYTAATGKSAGSKTQIDCQPVLLSDIY
                     FLKGLAYEQAGDLVKAVNCLETYVKMRPEDREGYFELANFLVKQNKLSQAEQYCLKAQ
                     KIKVTDADTRMQGQQVLLEIYRRQGKNRQAIAVADEMVAKDPDNVEAYFVRAQLYLSL
                     KDYAKALAEFNQIIKLDSSMAEGFAGRAEVNRILGRKAEALADFRRAASLDRAYKQAL
                     RDFELKLK"
     gene            1066..1539
                     /gene="ybaK"
                     /locus_tag="PKW73_02930"
     CDS             1066..1539
                     /gene="ybaK"
                     /locus_tag="PKW73_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNA72261.1"
                     /translation="MKTNAVRILDNHKVSYELKEYEVDPDDLRAESVAAKIGFPPEQV
                     FKTLVVRGDKNGVCLAVVAGDAQLDPKALAKLSGDRKCEVVALKEVQPLTGYIRGGVT
                     ALACKKDYPVYVDETVYLFEVISVSAGVRGTQIIVNPEDYIAVTRATVGAIGRKE"
     gene            complement(1549..3162)
                     /locus_tag="PKW73_02935"
     CDS             complement(1549..3162)
                     /locus_tag="PKW73_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNA72262.1"
                     /translation="MPRKKDKGSNNQHHDHNRDHGHDHKNHGHGHNHQGQSHDNLFSK
                     VPHLKHDLRDRAKLEMALNGLRAVFSPEVHEQVVRLLKQYDNGQGLKSFACEKTRDLR
                     HLLWSSIDNQDSRDLDQIEFVERTSHSSTKVLIAIADVDSFAAHKSPIDESARQNTTS
                     VYMGVINFPMLPDELSYDMTSLLPGQERLAVVYELHLGNHGHVVDSHVYRAFVTNKAQ
                     LDYKTIGLWLDGKIGVPREVAKVKGLTEQLLLQNHVKQQIFNLRQKHGSLFLRTVEAR
                     AVAVEGQVLDLELVEENPARDLIENFMIAANVAVSAYLDKKGFPSIKRIVRTPDRWPK
                     IVELAAAMGSHLPQSPDAPALQSFLLKMRDRDPLRFPDLSLRVVKLLGKGEYVLDPPG
                     ENNPGHFALAVNDYTHSTAPNRRYVDLITQRLLKAAMDGKTCPYSFDELSDLALECTR
                     KSSLAKKVERTLRKVAAASLLGDKIGNFFDGIVTGVKDEEVYVRLLKPPAEGRIVEGA
                     EGLEVGDEVRLKLLMVDEDNAFIDFACVH"
     gene            complement(3450..4385)
                     /locus_tag="PKW73_02940"
     CDS             complement(3450..4385)
                     /locus_tag="PKW73_02940"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNA72263.1"
                     /translation="MNHKTRTFRELLSTMFKSHPWHGVTAGADAPAFVTSYIEIAPPG
                     EVKLELDKGTGHLHVDRPQRFSSNCPTLYGFIPQTYCGDGISKLANKHAKNKTHRGDG
                     DPLDICVLTEKEFAHGDLLVRARVIGGLLMVDKGEADDKIIAVLDQDITYGHIRDISE
                     VPPGVIERLQHYFLSYKRPPSEAENCNTPVVTIDEVYGRKRAYEVISQSQADYVQTYG
                     TEESRLEELRQILSAGIQIGGSQAQNGNGGKESATVEKKSDKKGKKKTSQKAEKKSDK
                     KTKKKTAAKVEKKSDKKTKKTGVPEADKTEKAVVD"
BASE COUNT         1027 a         1121 c         1337 g         1191 t
ORIGIN      
        1 ccacagcgac ttgctgaatc gcagtaaggc ttatcgcgcc aaaggcatgt tgaaggaagc
       61 ggcacgtgat gcagcgctgg cagagaagta tgccttattg gaaaagctgc aggcctttcg
      121 tattcagttc aagctgaaag aagctaagca aactggtgat gaagccgtaa agcagttgcc
      181 ggaaagttct tatgccaatc tgctcggcgg tgtatccgcc ttgaattttg cttcttatag
      241 agaggctttt gaccgcttca gcaaggtgat cagcaagcat cctgattgca gtttcgcata
      301 ttactttcgc gctgatgcgg cacgaggtct gaagaagttt gcccaggccc agagcgatta
      361 cgacaagatt ttgagcctga agccgcgcta tgtggccatg agttataccg ccgccaccgg
      421 taagtcggcc ggcagtaaaa cccagatcga ctgtcagcct gtgcttcttt ccgatatcta
      481 cttcctgaag gggcttgctt atgagcaagc cggcgatctg gtcaaggctg taaattgtct
      541 tgagacctat gtgaaaatgc gcccggaaga tcgagaaggc tatttcgagc tggccaattt
      601 tctagtcaaa cagaacaagc tttcgcaggc ggaacaatat tgtttgaagg cgcagaagat
      661 aaaagttact gatgcggata cgagaatgca ggggcagcag gtgcttctgg aaatatatcg
      721 aaggcaggga aagaatcgtc aggccattgc cgtagctgat gaaatggtgg ccaaagatcc
      781 ggataacgtg gaagcatatt ttgtcagggc gcagctctat ctgagtctca aggattacgc
      841 taaagcactt gcggaattta atcagataat caaactcgat agttcgatgg ccgaaggctt
      901 cgccgggcgc gctgaagtca atcgcatatt ggggcgcaag gcggaagcgc tggctgattt
      961 tcgccgggca gcttccctcg acagagccta caagcaagct cttcgtgatt ttgagttgaa
     1021 gttaaaatag gaaaggcaga ggaccaggcg caggatattc gatagatgaa gaccaatgca
     1081 gtgcgaattc tcgataatca caaagtcagt tatgagttga aagagtacga agttgatccc
     1141 gatgatctgc gggccgagtc tgtagcggca aagatcggct tcccgcccga gcaggtgttc
     1201 aagaccctgg tagtgcgcgg tgataagaat ggtgtctgtc tggcagtcgt tgccggtgat
     1261 gcgcaattgg atccaaaagc actggccaag ctctcaggcg atcgaaaatg cgaagtggtg
     1321 gccttgaagg aagtgcagcc tctcaccggc tatatccgcg gcggtgtcac tgctcttgcc
     1381 tgcaagaaag actatccggt ttatgtggac gagaccgtct atctctttga agtgatctcg
     1441 gtttcggccg gggtaagggg cacccagata atcgtcaatc cggaagacta catagctgtt
     1501 accagagcca ctgtcggtgc gatcgggcgt aaggaatgaa ttgattgctc agtgtacgca
     1561 ggcgaagtcg ataaaggcgt tgtcttcgtc caccatcaag agtttcagtc ttacttcgtc
     1621 cccgacttcc agaccttccg ctccttccac gatacgacct tccgccgggg gcttcaacag
     1681 gcgcacgtaa acttcttcgt ctttgacacc ggtgacgata ccatcgaaga aattgccaat
     1741 cttgtcgccc aggagggaag cggccgctac tttgcgtaag gtgcgctcta ccttcttggc
     1801 caggctgctt ttgcgcgtgc attccagggc caggtcggat agttcgtcaa agctgtaagg
     1861 acaagtcttg ccgtccatag cggcttttag aaggcgctgc gtgattagat cgacgtagcg
     1921 tcggttcgga gcggtggaat gtgtgtagtc gttgacggcc agggcgaagt gtccggggtt
     1981 gttttccccg ggcgggtcaa gcacgtactc gcctttaccg agaagcttaa ccactcttag
     2041 cgacagatcg gggaagcgga gcggatcgcg gtcgcgcatc ttgagcagga agctttgcag
     2101 ggccggtgcg tccgggctct gaggaagatg ggaacccatt gccgctgcca attctacaat
     2161 tttgggccag cggtcgggcg tacggacaat gcgtttaatg gaggggaagc cctttttgtc
     2221 taaataggct gagacagcga cattggccgc tatcatgaag ttctcgatta agtctcgggc
     2281 cgggttctct tccaccagtt ccagatccaa gacctgacct tcaaccgcca cggctctcgc
     2341 ttctacggtg cgaagaaaga gggagccgtg tttttgtctc aaattgaaaa tctgctgctt
     2401 gacgtgattt tgcaaaagga gctgttcagt caaacccttt actttagcca cttctctcgg
     2461 tacgccaatt ttgccgtcca gccagagccc aattgtctta tagtccaatt gcgctttgtt
     2521 ggtgacaaag gcgcggtaga catggctgtc taccacgtgt ccatgattgc ccaggtgcag
     2581 ttcgtaaact accgccagtc gctcctgacc gggcaagagc gaggtcatgt cgtatgaaag
     2641 ctcgtcgggc aacatgggga aattgattac acccatatag acggaagtgg tgttctgtct
     2701 ggcgctttcg tcgatgggtg atttgtgagc ggcaaaacta tccacgtcgg caatggcgat
     2761 aagcactttg gtgctgctgt gactggttct ctccacgaac tcgatctgat caagatctcg
     2821 ggaatcttgg ttgtctatgg atgaccagag cagatgcctc aagtcgcgag tcttttcaca
     2881 agcaaagctc ttgagcccct gcccgttgtc gtattgtttc aagagacgca caacttgttc
     2941 gtgaacttcc gggctgaaga ccgcacgcag tccgttcagg gccatttcca gtttggcccg
     3001 atctcttaga tcatgcttga gatgcggcac cttgctaaag agattatcgt ggctctgccc
     3061 ttgatggttg tgaccatgac cgtggttctt gtgatcgtgc ccatggtcac gattgtggtc
     3121 gtggtgctgg ttattcgagc ctttgtcttt cttcctgggc aagtagggcc tccctttcca
     3181 gtcgaaaggc tatagtaaaa cctcggcacc gtcaatacgg aagcacagcg gcatcctgga
     3241 aaaggaatga atggctttgg ggaaaacgat cgaaaaacga tagagtatag tataaaaagt
     3301 catttaaaaa atatgtgccg cagcggctcc ttcggtagat catgagggta gggtggcaga
     3361 gattgtcgtt accgcctggc tggctcatgg aagtaggagg aggaaggctc tgaagccctc
     3421 cccctcaatg tttgtattca gccgccgaat cagtcgacca ctgctttttc agtcttgtcg
     3481 gcttcaggca cgcccgtctt tttggttttc ttgtcggact tcttctcgac ttttgcggct
     3541 gtcttcttct tggtcttctt gtcggacttt ttctccgctt tctgggaggt tttcttcttc
     3601 cccttcttgt cggatttctt ctcgactgtt gcgctttcct tgccgccatt gccgttttga
     3661 gcctggctgc cgccaatctg gatgccggcg gagagaatct gcctcagctc ttccaggcgc
     3721 gattcttcgg tgccgtaggt ttgcacgtag tctgcctggc tctggctgat cacctcgtaa
     3781 gcgcgcttgc gaccgtaaac ctcgtcgatg gtgacaaccg gggtattaca gttctccgct
     3841 tccgagggag gcctcttgta gctgagaaag tagtgttgca ggcgttcgat tacccccggg
     3901 ggcacttccg aaatgtcgcg gatgtgtccg taagtgatgt cctggtcaag cacggcgatg
     3961 attttgtcat cggcttcccc tttgtccacc atgagcagac caccgattac ccgtgcacgc
     4021 accagaagat cgccgtgggc gaattccttc tcggtgagaa cgcagatatc gagggggtcg
     4081 ccgtcgccgc ggtgggtttt gttcttggcg tgcttgttgg ccagtttgct gatgccgtca
     4141 ccgcagtagg tctggggaat gaaaccgtag agggtggggc aattggacga gaaacgctgt
     4201 ggtctgtcca catgcaggtg accagtgcct ttgtcgagtt cgagcttcac ttcgccgggt
     4261 ggggcgattt cgatatagct tgtgacaaac gctggggcat ccgctccggc ggtgacgccg
     4321 tgccagggat gcgatttgaa catggtcgag agcagctctc ggaaggtgcg ggttttgtga
     4381 ttcatagtca ggtccatatt ttagtcactg gtaccctaga ggattctagg aattggtgct
     4441 ctctgtgagc ggcagtttga agatctgctg gctggcctca taaaactgcc gaggtcgaca
     4501 caggtttgta tctcaagctg gaaactataa agtgtttgta ggtctggtta gtgaaaattg
     4561 gggaagattg aagaacttca tgttactgat tgaagatact ggaaatcaag aatgaaagta
     4621 ttaccggcag accggcgcag tggacgctgt ggcggaatcc tggcggaaag gctctt
//
LOCUS       DAOQNZ010000108         4673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_7875_length_4673_cov_4.464270, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000108 DAOQNZ010000000
VERSION     DAOQNZ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7875_length_4673_cov_4.464270"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2250
                     /locus_tag="PKW73_02945"
     CDS             <1..2250
                     /locus_tag="PKW73_02945"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNA72264.1"
                     /translation="MYESGRISPADLQEAFADGRELSSAAQGAVSEAALSTLADCLDK
                     RHDTGLLSLVNKQMIKWCSAYLDKTQAQWNLDGTDGFFASWRKLAAFDHSLTLYGVKN
                     WKQELSQLPDDSALALQLLLDKIGVAEGQVVDYLTRHLAQLVGFASHLKWRQAQGDSK
                     LLSDYLAMRLFYEWQYARPLLTRFYDPTIKPWTQLSPAAGGESAVASREGDYASLWQD
                     AYESNYRNRLLTELTESCRQVDDNKVSPQCQLVFCIDVRSEPVRRNLEKLGPYATFGF
                     AGFFGFAMQFKPLGSALSLDLCPVLIKPSKSVTETDRDGKCNGKVNLQSLLASAIQLR
                     KRLKSSLLGAFGLVEGFGLASALPLFARTIAPRQYENAVQAVGKQLCGHGHTEIDASA
                     FSLEEKIALARGNLKAIGIAKEVAPIVVLCGHKGHSQNNPYASALDCGACGGNAGGAS
                     ARLAALILNDKAVRLGLASQGVFVPDESVFVAAEHNTTTDNFEFYDVPELTTVQQQIF
                     ERLQADLEKAAALVREERRQCLPKSVIAGMNEPSCQSVDWAQVAPEWGLAGNAAFIAA
                     PRSVTAGVNLKGRSFLHSYDCRQDEDGSVLELIMTAPMVVAQWINMQYYLSTMDNEVF
                     GSGSKVVHNVVGDFGVMQGALSDLKIGLPAQSVMSAEGERMHEPMRLLVVIRATTEAI
                     DNVLKNHEHVASLVKNRWLHLVAMNPDSGEFSKADERLLWRPVRSGATVPVADLQKSL
                     PLLQQQSAS"
     gene            2268..3164
                     /locus_tag="PKW73_02950"
     CDS             2268..3164
                     /locus_tag="PKW73_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72265.1"
                     /translation="MGASYQHRLAALTSKHEKVRAMGLPFRAGLGLKLACAEEIDTDR
                     LGTFTGEVERQGTPRETVSAKARLGMEALGLPLGLASEGSFGAHPLLLFVPGTEELMV
                     FIDDELGIEVCERLVSTETNYASVSTASIDSLQGFLRQVKFPSHALIVRANQPQSVVS
                     VGYGAALNETGAGKIFKGLRSIEQLEKAIQQCQAQSVDGLALVQTDMRAHMNPTRMRL
                     IRKLAVKLNRRLQQLCELCQCPGWGLTGVEPGLPCGLCGFPSDVAKFEIYSCPRCEHK
                     AKLPRQDGLAHVDPGACQRCNP"
     gene            complement(3190..3648)
                     /locus_tag="PKW73_02955"
     CDS             complement(3190..3648)
                     /locus_tag="PKW73_02955"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNA72266.1"
                     /translation="MRKRFLGGFLAILPMGIVSSAILTHQHSAWPTATGTVVKATIEN
                     PPEQTTRGLYRLYLTYDFTVNGQKYTGRDYQTQDRDQARLKHEQEDRFPVGANLTVWY
                     DPQNPSQSDIKHSTDTMAAFFLVLAACAAFFAAKLLRGSPKAPVVPPEEY"
     gene            complement(3717..4199)
                     /locus_tag="PKW73_02960"
     CDS             complement(3717..4199)
                     /locus_tag="PKW73_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72267.1"
                     /translation="MVIKRLLKKLQTDVLGEVIRDYSDITQEPDGLNVRLKRQGKSAF
                     FAMSFASKELGKATFDVPVTTEAITALKALTEDIKLVRKNGAATSKEMRLRFRSGQID
                     LVVTYSYAKQGAEPGQRTAPLNSRTFKLLEEMLGDAEVVAAGNLSFDSEHWPAEPEDK
                     "
     gene            complement(4284..4523)
                     /locus_tag="PKW73_02965"
     CDS             complement(4284..4523)
                     /locus_tag="PKW73_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNA72268.1"
                     /translation="MRQHVTDPEICIGCSACELACPLKAIKSILGRYCVDASICEGCG
                     KCIEECPTGAADCFIEVGEAYSQEQQSEWTSLPQT"
BASE COUNT          927 a         1214 c         1350 g         1182 t
ORIGIN      
        1 atgtatgagt cgggacggat cagccctgcc gaccttcagg aagcctttgc cgacggtcgt
       61 gagctttcct ctgccgccca aggcgccgtg tctgaggccg cgctctctac ccttgccgac
      121 tgccttgata aacgccacga cacggggctc ctctcgctcg tcaataagca gatgatcaag
      181 tggtgctctg cttacctcga taagactcag gctcagtgga atcttgatgg aaccgacggc
      241 ttctttgctt cctggcgcaa actggctgcc ttcgatcact ctcttacttt gtacggtgtc
      301 aaaaactgga aacaggaact gtctcaatta ccggacgatt cagctcttgc tctgcaactt
      361 ttgctggata aaatcggtgt tgccgagggt caagtcgtcg actatctgac ccggcacctt
      421 gctcagctag tgggctttgc cagtcatttg aaatggcgtc aggctcaagg tgacagtaaa
      481 cttctcagcg attatctggc gatgcgccta ttctatgaat ggcaatacgc caggccctta
      541 ttgaccaggt tctacgaccc gaccatcaag ccctggacgc agctgagtcc ggctgccggc
      601 ggtgagagtg cggttgcttc aagagagggc gattacgcct ctctctggca ggacgcctat
      661 gagtcgaatt atcgcaaccg gcttctgaca gagcttacgg agtcgtgccg gcaagttgac
      721 gacaataagg tgagccccca gtgtcagttg gtcttttgta tagacgtgcg ctccgagcct
      781 gttcgcagaa atcttgaaaa gctggggcca tatgcaacct ttggattcgc cggcttcttt
      841 ggctttgcca tgcaattcaa gcctcttggt agcgcacttt ctctggatct gtgcccggtg
      901 cttattaaac ccagtaagtc tgtgacagag accgatagag atggcaagtg caacggtaag
      961 gtcaacttgc agtctttgct tgcttccgcc atacagttgc gcaaacggct caagagcagc
     1021 ctgcttggag cttttggtct ggtggaaggg tttggattgg cctcggcgct accactcttt
     1081 gccagaacca tcgcccccag gcagtacgag aatgccgtcc aggcggtcgg taagcagctc
     1141 tgcggccacg gacatactga aattgacgcc tctgcttttt ctctggaaga gaaaatagct
     1201 ctcgcccgtg gcaatctcaa agccatcggc atcgctaagg aggtggcacc aatagtggtg
     1261 ctatgcggtc acaaagggca ttcgcagaat aatccctatg cttccgccct ggattgcggc
     1321 gcctgcggcg gcaatgccgg cggtgccagt gccagattgg ccgctcttat tttgaatgac
     1381 aaagcggtgc gacttggtct ggccagccag ggtgtgtttg tcccagatga aagtgttttt
     1441 gttgctgctg agcacaatac aaccacagac aactttgaat tttatgatgt gccggaacta
     1501 acaactgttc agcagcagat tttcgagcgc ctgcaggcgg acctggagaa agccgctgcg
     1561 ctggtcagag aggagcgccg gcagtgtctg cccaagagtg taattgctgg tatgaatgag
     1621 ccttcctgcc agtcggttga ctgggctcag gtggctcccg agtgggggct tgccggtaac
     1681 gccgctttca tcgccgcacc tcgtagtgtc actgccggtg tcaatctgaa aggccgcagt
     1741 ttccttcatt cttacgattg ccgtcaggac gaagacggct ccgtgttgga actgattatg
     1801 actgctccca tggtggtggc tcagtggatc aacatgcaat actacctttc caccatggac
     1861 aatgaggtat tcggcagcgg cagtaaggtt gtgcacaacg ttgtcggtga tttcggcgtc
     1921 atgcagggtg cgctcagcga tttgaagata ggtcttcctg ctcagtcggt catgagtgcg
     1981 gaaggggagc gcatgcacga acccatgcgt ttactggttg ttattcgagc caccaccgag
     2041 gctattgata atgtgctcaa gaatcatgag catgtggctt ccctggtgaa gaatcgttgg
     2101 ctgcacctgg tggccatgaa tcctgacagt ggtgaattct ctaaggctga cgagcgctta
     2161 ctctggcgtc ccgtgcgcag cggtgccacg gtgcctgtcg ctgatctgca aaagtccttg
     2221 cccttgctgc agcagcagtc ggcgagctaa ggcggtcgtg cctgcctatg ggcgctagct
     2281 atcagcacag actggcggct ttgactagca aacacgagaa ggtgcgtgct atgggcttgc
     2341 ccttccgtgc cggtctggga ctgaaactgg cctgtgctga ggagatagat accgataggc
     2401 ttggcacttt caccggcgag gttgagcgcc agggaacgcc ccgagagact gtatcggcca
     2461 aagctcgcct gggcatggaa gcactgggct tgccgctcgg tctggccagt gaaggtagct
     2521 ttggagcgca tccgctcctt ctctttgttc ctggaaccga agaattaatg gtcttcatcg
     2581 acgatgaact gggaattgaa gtatgcgaac gattggtctc cacagagacc aactatgcta
     2641 gtgtcagtac cgcttccata gacagtttgc agggttttct caggcaggtg aaatttcctt
     2701 cccatgcttt gatcgtgcgt gccaaccagc ctcagtccgt ggtctccgtt ggttacggag
     2761 cggcccttaa tgagaccggc gcggggaaaa ttttcaaggg gctacgaagc atcgagcagt
     2821 tggagaaggc gattcagcag tgtcaggctc agtccgtgga cggcctggct ctggttcaaa
     2881 ctgatatgcg cgcgcatatg aaccccactc gcatgcgtct gattcgcaag ctggctgtta
     2941 agttgaaccg gcgcttgcag cagctttgtg aactgtgtca gtgtcccggt tggggactaa
     3001 ccggtgtgga gccgggcttg ccctgtggcc tttgtggatt tccttccgat gtggccaagt
     3061 ttgagattta ttcctgcccc cgctgtgagc ataaggcaaa actgcccaga caagacggtc
     3121 ttgcccatgt cgacccgggg gcatgccagc gctgtaatcc ctgagtttgg aaagaatttc
     3181 tttcgaatct caatattctt ctggtggcac cactggtgcc ttgggacttc cgcgcagtag
     3241 tttggcagcg aagaaagcgg cgcaagcagc caataccagg aagaaggcgg ccatggtgtc
     3301 ggtgctgtgt ttgatgtctg actggctggg attctgagga tcgtaccaga cggttagatt
     3361 ggctcccact gggaaacggt cttcttgctc gtgcttgagt cttgcttgat ctcgatcttg
     3421 tgtctggtaa tcgcgccctg tgtacttctg cccgttgact gtgaaatcat aagtcaggta
     3481 gaggcgatag agtcctctgg tggtttgctc gggagggttc tctatggttg ctttcacaac
     3541 cgtaccagtg gcggtgggcc aggctgaatg ttgatgggtg agaatagccg aggagacgat
     3601 gcccatgggt aggattgcta gaaaaccgcc caggaatctc ttgcgcattt gcgccctttg
     3661 cagttctctc tctttgctga aaaacacttt ctgggtttac cttttgttgg tcggctctac
     3721 ttgtcttccg gctcggcggg ccagtgttcc gagtcgaagc tgaggttgcc ggcggctact
     3781 acttccgcgt ctcccagcat ttcttctaga agtttgaagg ttctgctatt gagaggcgcc
     3841 gtgcgttgac cgggctcagc gccttgtttt gcgtaactgt aggttaccac aagatcaatc
     3901 tgaccgctgc gaaagcgcag gcgcatctct ttactagtag cggcgccgtt ttttcgcacc
     3961 agtttgatgt cttccgtgag agctttcagg gcagtgatgg cttcggtagt gaccggcacg
     4021 tcaaatgtgg ctttgcccag ttctttgctg gcgaagctca tggcaaagaa agcggacttg
     4081 ccctggcgct tcaagcgcac atttagaccg tcgggctctt gagtgatgtc gctgtagtcg
     4141 cgaatcactt ctccgagcac gtcggtttgc agcttcttga gcaggcgttt gataaccaag
     4201 ttgatgcagc cccttccatg ccctgtacgg tcctggcagc tatgataaca gatgccggaa
     4261 agggcctgcc aaggttcact atcctaggtc tggggcaggc tggtccactc cgattgttgt
     4321 tcttgcgagt aagcttcgcc tacttcaatg aagcagtcgg ccgcaccggt ggggcattcc
     4381 tctatgcact taccgcaacc ttcgcaaatt gaggcatcga cacagtaccg accgagtatg
     4441 gattttattg ctttgagcgg gcaggccagc tcgcaggccg agcagccgat gcagatttca
     4501 gggtcggtga cgtgctgtct catagatcaa gggcgagctt cttgagttcc cggctcactt
     4561 cctgcttcca ctcttccttg atttcgacgt tgtcggaatg tttcaagccg aagcgcttgt
     4621 gccactcatt gcgaagcgag tcgggtggac cggctgaggt ttcaacccga ggg
//
LOCUS       DAOQNZ010000109         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8006_length_4630_cov_3.822514, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000109 DAOQNZ010000000
VERSION     DAOQNZ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8006_length_4630_cov_3.822514"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKW73_02970"
     CDS             complement(<1..658)
                     /locus_tag="PKW73_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72269.1"
                     /translation="MLPIIDNHAHSLASNFLAAGEADFFSFLSESQHLQFRSEMRHSL
                     NGMLFEKALLQHAGVDNLSAYLAYRQQLGLASVSRLFADVCIKAVLLDYGFGHDLLSL
                     DDFALAAGVKVFGILRLESLFEELLRQYDQPGTLESALERHLAESGCVALKTICAYRG
                     GLSLLSQLTEENSQPETEVDEDESFQKCRRQVLKSGRIEGRGYYLRLLALSFHLAARR
                     G"
     gene            complement(661..2043)
                     /locus_tag="PKW73_02975"
     CDS             complement(661..2043)
                     /locus_tag="PKW73_02975"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNA72270.1"
                     /translation="MNEADTDLPGLLKKAADNGVELVRFFITDACGNLRGKVSSLSSA
                     LPRFESGIGLVKGTMAMNMLDQLQTDTGLGAVGEVRMVPEPETFTILPYAEASASVFC
                     RMEELDGRPWSHCPRAFLRRQVEEAEKLGFRVLAAYEPEFILGRESDGEFRPIDRGLC
                     FSSESMHAADHFITAVVKALKAQGIELEQYYPELGAGQHELSISPAQPMLACDQYLLL
                     KETVRALAQAQGLSVTFAPKRSASEPGNGAHLHLSLWTADGKQNLFYGDAMSSPDGDM
                     GLSQLALSFIAGVHQHLKALCALTCPSVNSYQRLKPSHWSSAFTAWGFENREAAIRVP
                     SVYRGRRESSTNIELKCVDSTINPYLALGAVLACGLDGIHRKLSPPAPVAVDPAEMEA
                     DFDLCALSGLEPLPRSLNDALTELEADLFLMKSLGAELVNTYIIVKTSEVAAMQDDPP
                     FAFSQHRQRY"
     gene            complement(2072..>2989)
                     /locus_tag="PKW73_02980"
     CDS             complement(2072..>2989)
                     /locus_tag="PKW73_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNA72271.1"
                     /translation="TEAQTGSSESTEEAPVDKVSDFLFARKYGDSRRFATAFCVLCRQ
                     QGLPSRVVVGFQPGTFNKLNGTYEVHGSDTFTWTEVFIPEYGWVAFDSTPDGVFPEQK
                     RDEGYNFTALVKALEEQLGLNEGEGLTPKKIFAIVTLAVSCIFLIVGIVYGIILLRRF
                     LKKRQEENRYRGPEWPVYLALLKDLKRLQIERAPTESQKQFVARVADTVSERVKQGLP
                     MPQDLPAALGDFFEVYDAVHFGNKEGMSELKERAKIVRGLARSSKISSKDLSPERSAR
                     VAANKGRGKEGPTAAESAVRGGRSKGPRA"
     assembly_gap    2990..3089
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3090..>4630)
                     /locus_tag="PKW73_02985"
     CDS             complement(<3090..>4630)
                     /locus_tag="PKW73_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72272.1"
                     /translation="LVAPVILTTGTEKKRCPSGSTNMTMALLPLLALIVFMAVPRSDN
                     FIDVIVSSFKSLNLNGLMRLFPDFSKKNEVKKNPYNPDQMKKVTLPPKELPADKKGEK
                     SKDKKPQAVSPEAKAKEAEKAAKKTPPAPPKKDPAKDAAKSPKSDPKSKAEAAAQKDA
                     AKKDAAKAAAKLDPKKLEEQARADAKKANEKLKAKGGAGGQGRSGKPKSLKPLKPLSS
                     AEAARLLSENFDELFPKDLNVDRVHNDQLAQLLLFRVTSTRLFFPRMTAFDYFDGEKW
                     QRSKDNINSKIVEVKSDGGPNDKPKLRTNVELDANGKPLKELTAEEEADIELASEKFF
                     KDAATDIGFGANESVHYLISKSEKSVYDLRQAQAFRVPRKLPYVDLTQSYEMLIDIGE
                     EVPSCWIPQSLGYGGSTAIVDDYGRISFPEKLKKGSTYKMTSCWPVNDLNAMREAAPI
                     TAEEEGSLREKLANYLQLPENIDPAVKEFGDKAIGGTGNWFVQAQKLCMAVRSNSTLS
                     NEDFVA"
BASE COUNT         1042 a         1228 c         1204 g         1056 t
ORIGIN      
        1 aacctctacg ggcggcgaga tggaagctca gggcaagtag tcttagataa taaccgcgcc
       61 cttctatgcg cccggatttg agaacctggc ggcgacactt ctggaaggac tcatcctcat
      121 ctacttccgt ctccggttga gagttctctt cagtcaactg cgagagcaag gaaagcccgc
      181 ctcgataagc acagatagtt tttaaggcaa cacaaccgct ctcagcaaga tgacgctcaa
      241 gggcagactc aagggtgcca ggttgatcat attgccgcaa cagctcttca aagagagact
      301 ccaacctcaa tataccgaac accttcaccc cggccgctaa agcaaaatca tcgagcgaca
      361 gcaagtcatg accaaagcca taatcaagaa gcacggcttt gatacagaca tcggcgaaga
      421 gtcgagaaac cgaggccaga ccaagttgct gcctgtaagc aagataggcg gagaggttgt
      481 ccacaccagc gtgctgcagc aaggctttct caaagagcat accgttgaga ctgtgtctca
      541 tttcagatct aaactgcagg tgctgggatt cacttaagaa ggaaaagaaa tcagcttcac
      601 cggcagccag aaagttggaa gccagactgt gggcatggtt gtctatgatg ggcagcaact
      661 ttagtaccgc tgacggtgct gggaaaaagc gaagggtgga tcgtcttgca tggccgccac
      721 ttccgaagtc ttgacgatga tataagtatt gaccagttca gcgcccaggc tcttcatcag
      781 gaatagatcc gcctccaact cggtaagggc atcgttcaga ctacgtggca gaggctccag
      841 acccgagagg gcacaaaggt cgaagtcggc ttccatctca gcgggatcaa ccgctaccgg
      901 cgcaggcgga ctgagcttac gatggatacc gtcgaggccg caggccagca ccgcccccag
      961 tgccaggtaa ggatttatgg tgctatccac acactttagt tcgatattgg tgctgctttc
     1021 ccgccgcccc cgataaaccg agggcacccg aatggcggct tcccgatttt caaaacccca
     1081 ggccgtgaaa gctgagctcc agtggctggg tttgagccgc tggtaagaat tgacggaagg
     1141 gcaggtgaga gcgcaaagag cctttaaatg ctgatgcact cccgctatga acgagagagc
     1201 caattgactg agacccatgt cgccatcggg cgaagacatg gcatcaccat agaacaggtt
     1261 ctgcttgccg tccgctgtcc agagagacag gtgcaaatgg gcaccgttac cgggctcgct
     1321 tgcgctccgt ttgggtgcaa aggtaacact gagcccctgc gcctgagcca gcgctcttac
     1381 ggtttccttc aaaagcaaat attgatcaca ggcaagcatg ggctgagccg gtgagatact
     1441 gagttcatgc tgaccggcac ccagttcggg atagtattgc tccagttcta tcccttgagc
     1501 tttcagagcc ttcaccaccg cagtgatgaa gtgatcggcg gcatgcatac tttcgctgga
     1561 gaaacaaagc cccctgtcga tggggcgaaa ttcgccgtca ctctcccgtc ccaggataaa
     1621 ctccggctcg taagcagcca gcactctaaa gcccagcttc tccgcctctt ctacctgcct
     1681 gcgcaggaag gcacgggggc agtgagacca gggacgccca tccagttctt ccatacggca
     1741 aaaaactgaa gccgaagcct cggcataggg cagaatagtg aaagtttccg gctccggcac
     1801 cattcttact tcgcctactg caccaagtcc ggtgtcagtc tgcaattggt cgagcatatt
     1861 catggccatg gtgcctttca caagaccgat accgctttca aagcgtggca gggcggaaga
     1921 aaggctggat accttgcctc gcaggttacc gcaggcatcg gtaatgaaaa aacggaccag
     1981 ctcaacgcca ttgtcggcag cctttttgag cagcccaggc agatctgtat cggcttcgtt
     2041 catctcttcc ttcctcggtc ggtacgccag gctaagcgcg tggtcccttg ctgcgtccgc
     2101 ctctgacggc actctcggcg gcagttggac cttccttacc gcgcccctta ttggcagcaa
     2161 cacgggcaga gcgctcaggg ctcagatctt tactggaaat tttcgaggat ctggccagac
     2221 cgcgcacaat cttggcccgc tccttcaact cgctcatacc ttctttgttg ccgaagtgca
     2281 cggcatcata gacctcgaag aagtctccga gagcagccgg caagtcttgc ggcatgggca
     2341 gcccctgctt caccctttca cttacggtat cggccactct agccacaaat tgcttctggc
     2401 tttctgtagg tgcccgctct atttgcagtc gcttcagatc tttgagcagg gccagataaa
     2461 ccggccattc cggtccacga taacgattct cttcctggcg cttcttgagg aagcggcgca
     2521 gcaggatgat accgtaaacg ataccgacga tgaggaaaat acaggaaaca gcgagagtga
     2581 ctatggcaaa aatcttcttg ggtgtgagac cttcaccttc gttaagacca agctgctcct
     2641 ccagagcctt gaccagagca gtgaagttat agccctcgtc gcgcttttgc tcggggaaga
     2701 cgccgtcggg agtggaatca aaagctaccc aaccatattc aggtatgaag acttcggtcc
     2761 aggtaaaagt atcggagccg tgtacttcat aggtgccgtt caatttgttg aatgtcccgg
     2821 gctggaagcc cactacaaca cgactgggca aaccttgctg gcgacagagc acacagaagg
     2881 ctgtggcaaa tcgtctgctg tcaccatact tgcgggcaaa gaggaagtcc gaaaccttgt
     2941 ccacgggagc ttcttcagtg gattcgctgg aaccggtttg cgcttccgtn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccacaaaatc ttcattggaa agagtggaat
     3121 tgctgcgcac cgccatgcaa agcttctgcg cctgcacaaa ccagttgccg gtgccaccga
     3181 tggccttatc tccaaactct ttcacagccg gatcaatatt ttcgggcaac tgcagatagt
     3241 tagccagctt ctctctcaga ctgccttctt cttcggcagt aatgggagca gcttcgcgca
     3301 tggcgttcag atcgttgacc ggccagcagg aagtcatctt ataggtgctg cccttcttca
     3361 atttctccgg gaaggaaatg cgaccgtaat cgtcaacgat ggcagtgctg ccgccgtagc
     3421 ccagagactg cgggatccag caagagggca cttcctcacc aatatcaatg agcatttcat
     3481 atgactgggt caagtctaca tagggcagtt tgcgaggcac gcggaaggcc tgggcttgac
     3541 gcagatcgta gacggatttt tcagatttgg agatcagata gtgcacactt tcgttggcac
     3601 caaagccgat gtcggtggct gcatccttga aaaacttctc ggaagccagt tctatgtcag
     3661 cttcctcctc ggcggtaagt tctttgagtg gcttgccgtt ggcatccaat tctacatttg
     3721 tacgcaattt aggcttatca ttgggaccgc catcactttt cacctcaacg atcttagagt
     3781 tgatgttgtc tttgctgcgc tgccattttt ccccatcgaa ataatcaaaa gcggtcatgc
     3841 gggggaaaaa gaggcgcgta ctggtgacac ggaagagcag cagctgggcc agttgatcgt
     3901 tatgcacccg gtctacattg aggtccttgg ggaagagttc gtcgaagttt tcagataaca
     3961 gacgagccgc ttccgctgaa gaaaggggtt ttaatggttt gagtgatttg ggcttaccgc
     4021 tgcgaccctg accgccggcg ccgcctttgg ctttgagttt ttcgttggct tttttggcat
     4081 ccgcccttgc ctgttcttcc agtttcttgg gatccagctt ggcggccgct ttggcagcat
     4141 cctttttagc agcatccttt tgagcagcgg cttccgcctt agacttggga tcagacttcg
     4201 gcgacttagc agcgtctttg gccggatctt tcttgggagg cgcaggcggc gtctttttag
     4261 cagccttctc tgcttccttg gctttggctt ccggactgac ggcctgtggt ttcttgtcct
     4321 tggacttctc acccttctta tcggccggca gttccttggg cggcaaggta accttcttca
     4381 tctgatccgg gttgtaggga tttttcttga cctcgttctt cttggaaaaa tcaggaaaga
     4441 ggcgcatcaa accattcagg ttcagactct tgaaactgga aacaatgaca tcgatgaagt
     4501 tatcggagcg aggcactgcc ataaagacaa tcaaagccag aagtggcaga agggccatgg
     4561 tcatattagt ggagccggat ggacagcgct tcttctcagt gcctgtggtc agtattaccg
     4621 gtgccacgag
//
LOCUS       DAOQNZ010000110         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8008_length_4630_cov_3.356284, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000110 DAOQNZ010000000
VERSION     DAOQNZ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8008_length_4630_cov_3.356284"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /gene="hypF"
                     /locus_tag="PKW73_02990"
     CDS             <1..1862
                     /gene="hypF"
                     /locus_tag="PKW73_02990"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNA72273.1"
                     /translation="RQPQREQVKEGERCRQGYENPLDRRFHAQPVACGSCGPGYFFQN
                     HGSDEKMRGVEGIKTCVEFIKQGKIVAVKGIGGYHLVLDASNREALKALRERKFRRDK
                     PFALMVADLELARELVELSDAAASALASVARPILLAAQRQPAHYKRAPQLDLALIAPD
                     NSDLGLMLPYTPLHHLLFKYGAPPVLVMTSANRSSEPIAFEDEQAKLDLFGIADGYLI
                     GERPIHRRMDDSVLSVSSLGVQVVRRGRGLAPDAVVRLPVSKPVLALGADLKNTITLC
                     IDGQCFVSQYIGDLEHESCYQAFQATAHDFLSMYNVASDQLTLVFDQHPQYLSSRFAD
                     RLEAAEKISVQHHRAHIASVLAEHKLYDEPVLGLAFDGTGYGDDGSIWGGEFFKGSLK
                     SGFDRVGRLAQTRLVGGDAAAKWPLQALAGFLCELKGLEVDFSAAPFNLPEKRFRLAM
                     QLAEKNLRCFPTTSVGRLFDAAAALCGFNLEQTFEAQAAIWLEHQGRLTTSSCRPYPF
                     VFDKLSRELRWQAVLTAMVSDRIKGRAVADLSRSFHLGLAVGAAEAAEAFCREYQLAT
                     VVLSGGVMQNLLLLELLEKQLLAAGLRVLVNRRVPANDGGISLGQAALAAFRT"
     gene            1986..2405
                     /locus_tag="PKW73_02995"
     CDS             1986..2405
                     /locus_tag="PKW73_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72274.1"
                     /translation="MNELVNALKDTESRNQVGDSARGAGSTASQSMLDEVWGKKCLSV
                     KGGCPDGPSELPDLPSFPGQIKPDPDYPIKPYEPLKPEPRRQCVQDDDGAIACGVPIG
                     PERPRSPRSGSIKEALPDYLDGQLRKLPGIILFDKQR"
     gene            2572..3048
                     /locus_tag="PKW73_03000"
     CDS             2572..3048
                     /locus_tag="PKW73_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72275.1"
                     /translation="MADESMRDEWKKCSCSNLKNPWFKHVSKEVQRPAKVVACVRTDA
                     PEPALERQVQTIDGYCRENNFELVRVFKDDGPAPGMGLSDALNSLSVVDGLVAVDLER
                     FVQHHDDRVMDLRPLLHQFFCTNKKYLITISEGINTSSAEGQKTAMQYIASEKENF"
     gene            complement(3045..3431)
                     /locus_tag="PKW73_03005"
     CDS             complement(3045..3431)
                     /locus_tag="PKW73_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72276.1"
                     /translation="MRFLLKLSLTALTFIFILPHIGGITFHGGFGTALLVSLLFGFML
                     WLVDLAAIAISAMLTITSFGMALLWLIPMWVLGYWLLPCIALKLVSTLAPQALTIVGW
                     TPAILAGLAMMIIGGVTSKLIGKKKD"
     gene            complement(3517..3723)
                     /locus_tag="PKW73_03010"
     CDS             complement(3517..3723)
                     /locus_tag="PKW73_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNA72277.1"
                     /translation="MKAISGRNQLTGRVISVKLGDIMAEIVMQVGENTITSVITRGSA
                     ESMELKVGDEVTALIKSTEVMLLK"
     gene            complement(3764..4321)
                     /locus_tag="PKW73_03015"
     CDS             complement(3764..4321)
                     /locus_tag="PKW73_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72278.1"
                     /translation="MKPAVLALALLCALTGLSLASPVPAKEAAPGQTQSKEFDDITSD
                     DFDGHRVLILKAQQALRHGAYDRAVKHMERAMTTGQKDVDVHYFYAMALEQKMEKAHF
                     RDQDSFSKCVNSWLKIYRGEVGDEKGLTIKGIGLAAGAFSNDEMAAQGKEHLLSLVGF
                     LPKAWESDAKYVKRAVATLGQASHH"
     gene            complement(4423..>4630)
                     /locus_tag="PKW73_03020"
     CDS             complement(4423..>4630)
                     /locus_tag="PKW73_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72279.1"
                     /translation="IGGLGGFVGPIIFGYMLQVTGIWTTCWMSFALLSLACLIWMHAT
                     INKMMRKQAPHLVDHIETAVSAGH"
BASE COUNT          997 a         1159 c         1345 g         1129 t
ORIGIN      
        1 agcgccagcc ccagcgtgag caggtcaaag agggcgagcg ttgccggcag ggttatgaga
       61 accccctgga caggcgcttt cacgcccagc cggtggcctg tggaagctgt ggtcccggct
      121 atttctttca gaaccatggc agtgacgaaa aaatgcgggg agtggaggga atcaaaactt
      181 gcgtcgagtt tatcaagcag ggcaaaattg ttgccgtaaa gggtatcggc ggctatcatt
      241 tggtcttgga cgcaagtaat agagaggccc tgaaggcact gcgagagcgc aagtttcgcc
      301 gtgataaacc cttcgccctg atggtggccg atctcgaact ggcccgggag ctggtggaac
      361 tttcagatgc tgccgcttcg gcgcttgcca gtgtggccag accgattctg ctggctgccc
      421 agcgacagcc ggcgcattac aaacgggcgc cgcaactgga tctggccttg attgctcccg
      481 ataacagcga tttgggtttg atgttaccct acacgccttt gcaccatttg ctcttcaagt
      541 atggtgctcc gccggtgctg gttatgacca gcgccaatcg cagtagtgag ccaattgcct
      601 ttgaagatga acaggctaaa ctggatctgt tcggcatcgc cgacggctat ctgattgggg
      661 agagaccaat tcacaggcgt atggatgatt cagtattgtc ggtgagttct ttgggtgtgc
      721 aggtggtgag gcgggggcgc gggctggcac cggatgcggt ggtgcgattg ccggtaagta
      781 agcctgtttt ggcacttggt gccgacttga agaatacaat aacactctgc atcgatgggc
      841 agtgttttgt cagtcagtat attggcgatc ttgagcacga gtcttgttat caggcttttc
      901 aggcaaccgc tcatgatttc ctttctatgt ataacgtagc atccgatcaa ctgacccttg
      961 tctttgatca gcacccccag tatttgagca gtcgctttgc cgatcggctg gaagcggcgg
     1021 agaagatctc cgtacagcac caccgggcac atatagcctc ggttctggct gagcataagc
     1081 tgtatgatga gcctgtactt ggtcttgctt ttgacggcac cggctatggt gatgacggca
     1141 gtatctgggg cggtgagttt ttcaagggct ctttgaagag tggttttgat cgggtcggta
     1201 gactggctca gacccgtctg gtgggcggag atgcggcggc gaagtggccg cttcaggctc
     1261 tagccggctt tctttgcgaa ttaaaggggc tggaagtgga tttttcggcg gcgcccttca
     1321 atctgcctga gaagcgcttt cgtctggcta tgcaactggc tgaaaagaat ctgcggtgct
     1381 tcccgaccac ctcggtcggt cggctgtttg acgcggccgc cgctctttgc ggtttcaacc
     1441 ttgagcagac ttttgaggcc caggctgcca tctggctcga gcatcagggg cgtcttacca
     1501 cttcttcttg ccgaccgtat ccttttgtct ttgataagct cagccgtgaa ttgcgatggc
     1561 aggcggttct caccgccatg gtcagtgatc gcatcaaggg cagagcggta gctgatctgt
     1621 ccaggtcatt tcacctgggg ctggctgtcg gtgcggccga ggcggccgag gctttttgcc
     1681 gggaatatca actggctacg gtggtacttt ccggcggcgt catgcagaat cttctcttgc
     1741 tcgagctttt agagaaacag ttgctcgccg ccggcttaag agttctcgtc aacaggcgcg
     1801 tgccggccaa tgatggcggc atcagccttg gacaggcggc actggcggct ttccgcacat
     1861 agactgcagc tttgtgcatg tcaacttcat gtcagcttgc cttgctaatt tgagggcatc
     1921 aagaacgccg gtccgccata agttgcggcg gcagtcagtc tcttattttc taaggatgtg
     1981 aagccatgaa cgagttagtc aatgctctta aggacaccga aagtcgcaat caagtcggtg
     2041 atagcgcacg gggtgccggg tcgacggcca gccagtcgat gcttgatgaa gtctggggca
     2101 aaaaatgtct ttcagtgaag ggcggttgcc cggatggacc gagtgaactg cccgatttgc
     2161 cttcgtttcc ggggcagatc aagccggatc cagactatcc gatcaagcct tacgaaccgc
     2221 tcaaacccga gccccggcgc cagtgcgtac aggatgacga cggcgccatt gcttgcgggg
     2281 tgccgatcgg tccggagcgt cccagaagcc cgcgcagcgg cagcataaaa gaagcgctgc
     2341 cggactactt agatggtcag ttgagaaaac tgcccggcat cattctcttc gacaagcagc
     2401 gctgagcacg ctcgtcccca ggtgcataga ggagccgctc cccggggctg gtcctctttc
     2461 tgcccttgcc atcatcccaa gagctgagtt ctatacttct ttggaggctt gtttttgctt
     2521 tccgggaggc gtagagtgtg gccagatttg gtatgtgaag aggctaaggt catggctgat
     2581 gaatctatgc gggatgaatg gaaaaaatgc tcctgcagca atttaaaaaa tccctggttc
     2641 aagcatgtgt caaaagaagt gcaaagaccg gctaaggtcg tggcttgtgt tcgaaccgat
     2701 gcgccggaac cggccctgga aaggcaggtt cagactattg acggctactg tagggagaac
     2761 aacttcgaac tggtaagagt cttcaaagac gatggaccgg cgccaggcat gggtttgtcc
     2821 gatgcgttga acagtctgtc cgtggtggat ggtcttgttg cggtagatct agagcgcttt
     2881 gtgcagcacc acgatgatag agtcatggat ctgcgaccgc ttctgcatca gttcttttgt
     2941 acaaacaaga aatacttgat cacaatcagc gaaggcataa acacaagctc ggcagaaggt
     3001 cagaagaccg ccatgcagta tatcgcttcc gagaaagaga atttttagtc tttctttttg
     3061 ccgattagtt tgcttgtcac gccgcctatg atcatcatgg ccagaccagc cagtatggct
     3121 ggtgtccagc ctactatggt gagggcttgc ggcgctaatg tggacaccag ttttaaggca
     3181 atgcagggca agagccagta gcccagcacc cacatgggta tgagccagag cagcgccatg
     3241 ccgaaggatg taatggtcag catggcggaa atagcaatgg cggctagatc taccagccag
     3301 agcatgaagc cgaaaagaag agagaccaga agggctgtac cgaagcctcc atgaaaagta
     3361 atgccgccta tatgaggcag tatgaagatg aaggtgaggg ctgtgaggct gagtttaagt
     3421 aaaaatcgca tggcatgctc tggctggaaa aatcgggcga gtaaattggg tctccagtct
     3481 gcattctata tcttttcaag ggtggaggcc gggaatttac ttgagaagca ttacttccgt
     3541 cgactttatt agggcagtca cctcatcccc taccttcagt tccattgatt cagcagaacc
     3601 cctggtgatc accgatgtaa ttgtgttctc tcctacttgc atgactatct cggccatgat
     3661 gtcacccagt ttgacggaga taacccttcc ggttaactga ttgcgtccgc taattgcttt
     3721 cacttatctt cctccctgca caatttttat tttgtcgtcc gggctagtgg tgggacgcct
     3781 gtcccagagt agcgacggct ctcttgacat atttggcatc cgattcccag gctttcggta
     3841 gaaaacccac caggctcaag agatgctcct tgccctgggc ggccatttca tcgttggaga
     3901 aagcgccggc cgccagacct atgcctttga tggtgagtcc tttttcgtct cccacttcgc
     3961 cccgataaat tttcagccag gaattgacgc acttagaaaa gctgtcctga tcccgaaaat
     4021 gagccttttc cattttctgt tccagggcca tggcgtagaa ataatgcacg tccacgtctt
     4081 tttgacccgt ggtcatggcc cgctccatgt gtttgacggc ccggtcataa gcaccgtgac
     4141 gcagggcttg ttgggctttg agaatcagca cccgatgtcc gtcaaagtcg tcggaggtga
     4201 tgtcgtcgaa ctctttggat tgggtttgac cgggtgcagc ttctttggcc ggaacggggg
     4261 acgcaagact gagaccggtg agggcgcaga gcagtgccag agccagtacc gccggtttca
     4321 tcgatgatcg cctagagttc atagtcttac cgccaagctc ccttaaggcg ctattgtagc
     4381 aaagcggtac ggctgaactg agggggaatt catggaccag ggttagtgac cggcacttac
     4441 cgccgtttct atgtggtcga ccaggtgggg ggcctgttta cgcatcattt tattgatcgt
     4501 agcgtgcatc cagatgaggc aagccaggct cagtaaggcg aatgacatcc agcaggttgt
     4561 ccagatgcct gtcacttgta gcatatagcc gaagatgatt ggtccgacaa agccgcccag
     4621 accaccgatt
//
LOCUS       DAOQNZ010000111         4623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8030_length_4623_cov_3.709063, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000111 DAOQNZ010000000
VERSION     DAOQNZ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8030_length_4623_cov_3.709063"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PKW73_03025"
     CDS             <1..497
                     /locus_tag="PKW73_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72280.1"
                     /translation="YSLELSMSSADLLARLKEDTTSGFGLMLRSKPFYGKVSADGFKL
                     RSTRVSTRNSWSPQVVGRVKAGSRENTCLVEFSFDLPTSIKIASPLVFLSFLVVLIAL
                     LPELTSPGKAVFLLFQFLFLATIIPLSLYMGLRLGKKDKQHILEYFQNLPGAKLSVQK
                     RLIE"
     gene            complement(543..1160)
                     /locus_tag="PKW73_03030"
     CDS             complement(543..1160)
                     /locus_tag="PKW73_03030"
                     /inference="COORDINATES: protein motif:HMM:NF018693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72281.1"
                     /translation="MTAETAVSEAKPALEDKPLNLTLPREALQEDLSRQSWQLMGDAQ
                     GAQGKSIWGGSEKLKDAGISFNQEEGKLKIDLQNKHDTWLQLGALSYHQNRQANYNET
                     NYGVGILRRLDDESAVAFGYYRNSIHKDSFYAAYHYTPYQYGPVKLGLQAGLISGYKA
                     LKGFPTPMLLPLASIEGKNVAVDLTCIPPLGGVSAVCAAQMRVKF"
     gene            complement(1309..2055)
                     /locus_tag="PKW73_03035"
     CDS             complement(1309..2055)
                     /locus_tag="PKW73_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72282.1"
                     /translation="MTGNPENKVILDNWFSRSVNELRRDGGAGTNSSARNRAVPPVNS
                     QNSKLSADLSRKRLATMHNMQALKPPGQETQTRAPEIVANTLLDFDTMQIFIAQASQR
                     AFLSQVNNSTVPLSFLVVGFHDLFSLAGVFGMPAYEEALGFASRAIIHMGKPEEKTAL
                     LSSDKLLVMLIDRPEEETVQLADRIGVFFNSQPFYAGPQSIFLVPSIGGSFYGKDGLT
                     WQELVVKANQAADFVIQRGGNSYAGASSLG"
     gene            2200..2709
                     /locus_tag="PKW73_03040"
     CDS             2200..2709
                     /locus_tag="PKW73_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72283.1"
                     /translation="MSIEPGSAADWAAALTNTVVLLFIYRQLKLMNVQMIQNDEQERF
                     RRSLEFIKLYKEQLEQTDSLLPCELLNGDFLEKEPSGEDFQKLLELFYRPRVRLFTLL
                     NLLMEHQEVEERLLFNYLIDEFNNFLAIGVKADGSAAFMQGTGARLSILLTAWGSQVK
                     ARKLLFPVG"
     gene            2807..3253
                     /locus_tag="PKW73_03045"
     CDS             2807..3253
                     /locus_tag="PKW73_03045"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HNA72284.1"
                     /translation="MLNETAPDFALEATNGEQVKLSSLKGAFVVLVFYPANDTPTCNA
                     QLAEMSLSAQELLARNALVFGVNTASASRSGDYCLRKRLTFPILSDPGGAVARQYKAF
                     TAWIGLNRRTVVVVSPEGSICFYEKGKPPASKILAVIDNLRAPAEA"
     gene            3266..4525
                     /locus_tag="PKW73_03050"
     CDS             3266..4525
                     /locus_tag="PKW73_03050"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA72285.1"
                     /translation="MSLRIILSLCLALSCLCPVVQAQEEAATGPVADKWAVVIGISKF
                     KNPTLNLKYSAKDARDFRDFLVNSCHFAPDHVLLLTDEAATKEQILDVLGDSWLPRVT
                     LPEDLCVIYFSSHGSSSDMDLRGTNYVVVHDTNPEKLFTTGISIQGLATTIKERVHSK
                     RVLIVLDACHSGGASDESKGLRRVGGVDASLMAQGTGHAVICSSQKSESSWESKQYAN
                     GVFTRSLIESFAVNGEKTTLKEAFGHLKESVQRQVVGERGVTQTPVLEASKWSGPELV
                     LAVPPVRPRPLPEAAKIVLNQPEKNVVSANLNSAAAASNISSSPVTLKASVPDIAGDY
                     MGSNGLHYHYWQKGSRCGFEMPAWGETSKGIITENESKNGFVLVSSWSGLVNGSGTAD
                     LECDSALRVIKIKSQDGTVLTRVDVAK"
BASE COUNT          996 a         1245 c         1249 g         1133 t
ORIGIN      
        1 ggtactccct tgagctttct atgtccagtg ccgacctctt ggcccgcctg aaggaggaca
       61 ccacctccgg tttcgggctg atgctgcgca gcaaaccctt ctacggcaag gtcagtgccg
      121 acggcttcaa gctgcgctcc acccgcgtca gcacaagaaa ttcctggtcg ccgcaggtag
      181 tgggccgtgt caaagccggc agccgtgaaa acacttgcct ggtggaattc agctttgacc
      241 tgcctacctc catcaagatt gcctcaccgc tggtctttct ttccttcctg gtggtgctga
      301 tcgccctttt acctgagctt acttccccag gcaaagccgt ctttctactc tttcagtttc
      361 tcttccttgc caccatcatt ccgctttctc tctacatggg tctgcgcctc ggcaaaaaag
      421 acaagcagca tatcctcgag tacttccaga acttgcccgg ggccaaactt tctgtgcaga
      481 agcgtttgat tgagtaaatg atttcgctca gccaatattc tcagtaaact ttcctcttgc
      541 cctcagaact taacccgcat ttgcgccgca catactgccg atacgcctcc cagaggcgga
      601 atgcaggtga ggtcaacggc tacgttctta ccttcaatag aggccagcgg caagagcatt
      661 ggtgtgggaa agcctttcaa agccttgtaa ccagagatca aaccagcttg cagacctagt
      721 ttgacaggtc cgtattgata gggggtgtag tggtaggctg cgtaaaagct gtctttatga
      781 atgctgtttc ggtaatagcc aaaggctaca gcactctcgt catcgaggcg ccgcaggatg
      841 cctacgccgt aatttgtttc attgtaattg gcctgccgat tctggtgata agaaagtgcg
      901 cctaattgca gccaggtatc atgcttgttt tgcagatcaa ttttgagttt gccttcctcc
      961 tggttgaagc ttatgccggc atctttcaat ttctccgaac cgccccaaat gctcttgcct
     1021 tgcgctcctt gagcgtctcc catgagctgc caggactgtc tggagaggtc ttcttgcaga
     1081 gcctctctgg gcagggtcag attcaaaggc ttatcttcaa gcgcaggctt cgcctcgctg
     1141 acggcagtct cagcagtcat ttatccaagg acctcggatt gtctgttttt gggggaggtc
     1201 ccgaggatat atccgcccct atatgacagc aatggtaaga atactaatta ctacccaagc
     1261 ttagaaacag atataggttg tgacagaaat gtggcatggt ctgcgggttt agcctaagga
     1321 tgaggccccg gcataactgt tgccgccgcg ttgaatgacg aagtctgccg cttgattggc
     1381 cttgaccacc aactcctgcc aggtgagacc gtctttaccg taaaagctgc cgccgatgct
     1441 gggtaccaga aagatgctct ggggaccggc atagaaaggc tggctgttga aaaacacgcc
     1501 gatacgatcg gctagttgga cggtttcttc ttccggccgg tcgatgagca tgacgagtaa
     1561 cttatcactg gagagcaagg ccgttttttc ttccggcttg cccatgtgaa tgatggctct
     1621 ggacgcgaac cctagcgctt cttcgtaggc cggcatcccg aacacaccag ccaggctgaa
     1681 gagatcgtgg aaacctacca ccagaaaaga taagggcacc gtactgttgt ttacctgact
     1741 gagaaaggct cgctgactgg cctgggcaat gaaaatttgc atggtatcga agtcgagcaa
     1801 ggtgttggcc acaatctcgg gagcccgggt ctgggtctcc tgaccgggcg gtttcagcgc
     1861 ctgcatgttg tgcattgtgg ccagtcgttt gcggcttaga tcggctgaga gtttggaatt
     1921 ctggctgttg accggcggaa cagcgcggtt tctggcgctg gaattcgtac cggctccgcc
     1981 gtcccggcgc aattcattga ccgagcgact gaaccaattg tcgaggataa ctttattttc
     2041 cgggtttccc gtcatgatgg ttgccacctt ctctgcccta ctttcatttt attccccgtt
     2101 tgtgatcttt ttgtttgccc cccgccccgc tgcctgcttt gttacagttg ccacgatgtt
     2161 aaaattgcac atcactgaga agggtggtga gtttcgctta tgtccattga acctgggtct
     2221 gctgctgact gggctgcggc tctgacgaac acggtggtgc tcctgttcat ctacagacag
     2281 ctcaagctca tgaacgtaca gatgattcag aacgatgagc aggagcgctt tcgccgttct
     2341 cttgagttta ttaagttata caaagagcag ttagagcaga ccgacagcct tctgccctgt
     2401 gagctattga acggtgattt ccttgagaag gagcccagcg gcgaggattt ccagaaactg
     2461 ctggaactct tttatcgccc ccgggtgcgg ctctttaccc ttttgaattt gctcatggag
     2521 caccaggagg tggaagagcg cctgctcttt aattatttga tcgatgaatt caataacttc
     2581 ctggccatag gggtgaaggc ggacggctcg gccgccttta tgcagggcac cggcgcccgt
     2641 ttgagtattt tgctcacagc ctggggctcc caggtgaaag cccggaaact tctctttccg
     2701 gtgggttaaa atgccaggaa ggaaaagtcg cactttgccc gtgctgacca accataccga
     2761 atactaaggc aagtattgcc ctgacgaaga gttagagtta agaaaaatgc tgaacgagac
     2821 cgcccctgac tttgccctgg aagctacaaa tggtgagcaa gtaaagcttt ccagcctgaa
     2881 gggggccttt gtggtgctgg tcttttatcc agccaatgac acccccacct gtaacgccca
     2941 gcttgctgaa atgagcctct cggcccagga actgctggcc cgcaatgccc ttgtctttgg
     3001 ggtcaacacg gcttcggcct cccgctccgg cgactattgt ctgcgcaaac gactgacctt
     3061 ccccattctc tccgacccgg gcggcgccgt ggccaggcag tacaaggcct ttaccgcctg
     3121 gatcggctta aatcgtcgca ccgtggtggt agtctccccc gagggttcca tctgcttcta
     3181 tgagaagggt aagcccccgg ccagtaagat tttggcggtc atcgataact tgcgcgctcc
     3241 ggcggaggct tgaggcttag aactgatgag tctgaggata attctgagtc tgtgcctggc
     3301 cctttcctgc ctctgtccgg tggtacaggc ccaggaagaa gcggctaccg gaccggtggc
     3361 ggacaaatgg gcggtggtta tcggcatcag taagttcaag aatcccaccc tcaacctcaa
     3421 gtattccgcc aaggatgccc gggattttcg cgacttcctg gtcaattcct gtcacttcgc
     3481 cccggatcac gtgctcttgc tcaccgatga agcggctaca aaagagcaga ttcttgatgt
     3541 attgggcgat agctggctgc cgagggtgac tctgccagag gacctttgcg tgatttattt
     3601 ctccagccac ggcagttcct ccgacatgga cctgcgcggc actaattatg tcgtcgttca
     3661 cgacaccaac cccgagaagc tattcacaac cggcatttct attcaggggc tggccaccac
     3721 catcaaagaa cgggtgcaca gcaaaagggt cttgatcgtt cttgatgcct gccattccgg
     3781 cggcgccagt gacgaaagca agggtttgcg gcgggtaggc ggcgtggacg cctccttgat
     3841 ggctcagggc acaggtcatg cggtcatctg ttccagccaa aaaagcgagt cttcctggga
     3901 atccaagcaa tatgccaacg gcgtattcac ccgctctttg atagagtcct tcgccgtcaa
     3961 cggcgagaag actaccttga aggaagcctt cggtcatttg aaagagtccg tgcagcgcca
     4021 ggttgtggga gaacgggggg tgacccagac ccctgttttg gaagccagca aatggagcgg
     4081 accggaactg gttctggctg tgcccccggt cagaccgcgc cccctgccgg aagctgccaa
     4141 aattgtgctc aatcagcccg agaaaaatgt cgtatctgcc aatctgaatt cggcagcggc
     4201 cgcctctaat attagctcgt cgccggtaac actaaaggct tccgtacctg atatcgcggg
     4261 tgattacatg ggttccaacg gcttacacta ccattactgg caaaagggca gccgctgcgg
     4321 cttcgagatg cctgcctggg gcgagaccag taagggaatc atcacggaaa atgagagtaa
     4381 gaacgggttt gtgctcgtat catcctggtc tggtctggtg aacggcagcg gcacagctga
     4441 tctggaatgt gattcagccc tgcgggtgat caagatcaaa tctcaagacg gtacagtatt
     4501 aacaagagtg gatgtggcaa aatgagccag aggaaaacta ccatggttgg caaaaaggga
     4561 agcattccgg tgggccggct caagtctgtg gtttgcagca tgctggttct tctggcatgc
     4621 cag
//
LOCUS       DAOQNZ010000112         4591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8126_length_4591_cov_4.534832, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000112 DAOQNZ010000000
VERSION     DAOQNZ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8126_length_4591_cov_4.534832"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2209
                     /gene="priA"
                     /locus_tag="PKW73_03055"
     CDS             <1..2209
                     /gene="priA"
                     /locus_tag="PKW73_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNA72286.1"
                     /translation="LQLLQASSRGLLSLSTLRQKFEGAGRSLRPKLTVADFHKALKEL
                     CARGVTEIKEEEEGGSSVKTVAVLTPGQVPGRTSRQKEVKALLEELSTCTLPAFLEKS
                     GATRSTIDKMVQEGQLVLTRQEVVRESLGQLPAALVDKHRQPPVLTAEQEQVFAVLSR
                     ALEGTLARQAPFAHCEEPWLLHGVTGSGKTEVYLRLISACLAENRTALFLVPEIALTP
                     QLSGRLRARFGDLVSVWHSAVSAGERYDTWRRLRAGSVKVLLGARSAVLANMPDLGLI
                     VLDEEHDASYKQSSPAPRYNARDLALEKARRAGAMVLLGSATPDLGSFERARAGDRLL
                     TMVNRVFQPVMPEVKIVDMRNKDLGPVEKGIFSNALKAALKDRLEKAEQVVLLINRRG
                     FASHIFCQACGNVCRCKNCSVSLVMHSFQGRPKKTPAPPLLGAAEEPGDLSLLAEAED
                     IDAGPSLLKGGGYLACHHCGFRQPLKPDCPSCGSPFLKVAGVGTQKVELEVLALHPTA
                     RVVRLDSDIARKKGAYEAVLAKFSAGEADVLIGTQMVAKGLDIPGVTLVGVLLADAAF
                     NLPDYRSVERGFQLLTQVAGRAGRGDRPGSVILQTYTPELPVLKLAAHHDYHGFFQPE
                     LESRRLFDYPPFARLTRLLVSGEDLLLVESVIEILAEELSRLLEDEDEEGQVKMLGPA
                     PCLIERIKGRYRYHLIIKNRSERLQRLLLDYLRHRRFGPEVNLAVDVDALDLV"
     gene            2272..3675
                     /locus_tag="PKW73_03060"
     CDS             2272..3675
                     /locus_tag="PKW73_03060"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNA72287.1"
                     /translation="MGISSSDLKKVSRKVSKKHGSVSGGATAVTFSYYFCFALLLLLA
                     LPLLLFKKKARAGFSEKLGIIPGRIKSKQRQLAGCLWFHAVSVGEFNAVFPLIKAFKK
                     EHPAAGLVVSTTTGTGQALAREKCRDLAEVIYFPYDLPFSNKAWLDALSPSLVAITET
                     EIWPGFTYECKKRGIMLTVVNGRISPRSFKGYKRFRAFFGPVLRRFDNIGVQSLEEAE
                     RYRAIAGQEAKITVLGNLKFDGLTPPSEETRAALRALVNIDAGAAPVVVAGSTHEGEE
                     AAFLSYLKEHGKKREFHLILVPRHPERFQHVARLIEEAGFVVRRYSKQEGFLPDLAVS
                     KQVYLLDAIGKLFDFYSLADIAFVGGTLAPIGGHNIMEPYAYGVPVVVGPHIEKTRDV
                     AQALSQAGALTMVSSGEALTPALSLLLSDESERLSRGERGRGILIASQGAVGKAMHML
                     EATLSKNNPSHELSGLN"
     gene            3675..>4591
                     /gene="lpxK"
                     /locus_tag="PKW73_03065"
     CDS             3675..>4591
                     /gene="lpxK"
                     /locus_tag="PKW73_03065"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNA72288.1"
                     /translation="MTTAVQALLAPLAGLYGLGAYARLLSYSLGIKSRKKADAFVLSV
                     GNLTVGGTGKSPVVIDIAARLSRRGLKVAVLSRGYGRRGKTPYVVVSDGSGKPRCSVE
                     ESGDEPYLIATAVPAACVIVGASRVQTQALAIQKYGAEVLILDDGFQHLPMERDMDLV
                     LWDYNDDPGTAKLLPAGRLREPFSALSRASHIVVTKVPEVPDQAYLEGLLKTLRSYNP
                     EAEMSTVSFLIDCVVNALDLGRSLPPSELSGKRWLALSAIARPESFQASLRQLALDVV
                     EVMTRPDHSWFSERDIRQLSAALESGSVDG"
BASE COUNT          920 a         1218 c         1389 g         1064 t
ORIGIN      
        1 tttgcaactg ctccaagcct cctctagagg gttgctcagc ctctccactt tgaggcagaa
       61 atttgagggc gctggtcgca gtctcagacc gaagcttact gtggctgatt ttcacaaggc
      121 cttgaaggaa ctttgcgccc gcggcgttac cgaaatcaaa gaggaagagg aagggggcag
      181 ttctgtaaaa accgtggctg tcctcacgcc gggacaggtt cccgggcgca cctccagaca
      241 gaaggaagta aaggctctcc tggaagaact ctccacctgt accttgccgg cttttcttga
      301 gaagagcggc gccacccgct ctaccattga taagatggtg caggaagggc agttggtctt
      361 gaccaggcag gaagtggtgc gtgagagtct gggacaactg ccggcggcac tagtcgataa
      421 acaccggcag ccgcctgttc tcactgccga gcaggagcag gttttcgcag ttttatcccg
      481 ggccctggaa gggacccttg ctcggcaggc cccttttgcc cactgcgagg agccctggct
      541 cttgcacgga gtgaccggat ccggcaaaac cgaggtctat ctccgcctca tatcggcctg
      601 cctggccgaa aatcgcaccg cccttttcct tgtccctgag attgccctga cgccccaact
      661 ctcggggcgg ctgcgggctc gcttcggtga tctggtttcg gtctggcaca gtgccgtttc
      721 ggccggggag cgctacgata cctggcggag gctgcgggcc ggcagtgtca aagtcttact
      781 gggtgctcgt tcggccgtcc tggccaatat gcctgatctg ggtcttatag tgctcgatga
      841 agagcacgat gccagttaca aacagagcag cccggcccct cgctacaatg cccgggatct
      901 ggcgctggag aaagccagac gcgccggtgc catggttcta ctgggcagtg ccacccctga
      961 tctgggcagt ttcgagagag cgcgcgccgg tgatcgcttg ctgaccatgg tcaaccgagt
     1021 cttccagccg gtcatgccgg aagtgaagat tgtggatatg cgcaacaagg atctgggacc
     1081 ggtggagaag ggcatctttt ccaatgcctt gaaagcagcc ctgaaagacc gtcttgaaaa
     1141 agcagagcag gtggtgctgc taataaatag acgcggcttt gccagtcata ttttttgcca
     1201 ggcctgcggc aatgtctgtc gctgtaagaa ttgcagcgtc tctcttgtga tgcattcctt
     1261 tcaaggtagg ccgaaaaaga ccccggcgcc gcctcttttg ggagcggcgg aggagcccgg
     1321 tgatttgagt cttctggcgg aagcggagga catcgatgcc ggtccttctt tactcaaggg
     1381 cggtggttat ctcgcctgcc atcactgtgg tttcaggcaa ccactcaagc ctgactgtcc
     1441 atcctgcggc agtccttttc tcaaggtggc gggggtcggc acccagaaag tggagttgga
     1501 ggtgctggcc ctgcatccca ccgcccgggt ggtgcgcctc gattcggaca tcgccaggaa
     1561 gaagggtgcc tatgaagcag tattggccaa gttcagcgcc ggggaagcgg atgtactgat
     1621 cggcacccag atggtggcca aagggcttga tattcctggc gtgacactgg tgggggtttt
     1681 gcttgccgat gccgctttca acttgccgga ttatcgctcg gtggagcgag gtttccagct
     1741 actgacccag gtagcggggc gggccggacg tggggatcgt cccggttcgg ttattttgca
     1801 gacctataca ccggaattac cggtgctgaa attagctgcc catcacgatt atcacggctt
     1861 cttccagccc gagctggaaa gccgcagact tttcgactat cccccttttg cgcgcctgac
     1921 ccggttgctt gtctccggtg aagatctgct tttggttgag tcggtaattg aaatcctggc
     1981 tgaggaatta agccgccttc tggaagatga ggatgaggaa gggcaggtga aaatgcttgg
     2041 accggcccct tgtttgatag aaaggattaa gggacgctac cgttaccatt tgatcatcaa
     2101 gaatcggtcc gaacggctgc aaaggcttct cctggactat ttgcgccacc ggcgcttcgg
     2161 accggaggta aatctggcgg tggatgtgga cgccttggat ctggtctagg ggtttatact
     2221 tagtgcttgc tttgctaaga gcagcgttta agacaggacc tccggaccat tatgggcata
     2281 tcctccagtg atttgaaaaa agtttccaga aaagtttcta aaaagcatgg cagtgtttcc
     2341 ggaggggcta cggcggttac cttcagctac tatttttgct ttgccttgct cttgcttctg
     2401 gctttgcctc tcttgctttt caagaaaaag gccagggccg gtttcagcga gaagctaggc
     2461 atcattcccg ggcgcatcaa aagcaaacag cgtcaactgg ctggttgtct ctggtttcac
     2521 gctgtttccg tgggagagtt taatgctgtt ttccctctca tcaaggcatt taagaaggaa
     2581 catcccgctg ccgggcttgt ggtttcaact actaccggca ccgggcaggc actggccagg
     2641 gaaaaatgcc gggatctggc cgaggtgatc tatttcccct atgatctgcc tttctccaac
     2701 aaggcctggc tggacgccct ttcacccagt ctggtagcca taaccgaaac tgaaatctgg
     2761 ccggggttta cttatgagtg caagaaacgg ggcatcatgt tgactgtggt gaacggccgt
     2821 atttcgccgc gctctttcaa aggttataag cgtttccgcg ctttcttcgg tccggtactg
     2881 aggcgctttg acaatatcgg cgtgcaatcg ctggaagagg ctgaacggta ccgggccatt
     2941 gccgggcaag aggccaagat cactgtgctt ggtaatctca agttcgacgg gctgacaccg
     3001 ccttcggaag agacgagggc agccttgaga gccttagtga atatagatgc cggtgccgca
     3061 ccggtagtgg tggcagggtc aacccatgag ggggaagaag ccgcctttct cagttacctg
     3121 aaagaacacg gtaagaagcg cgagtttcac ttgattctag tgcccaggca tcccgagcgc
     3181 tttcagcacg tggccaggct tattgaggaa gccggatttg tggtgcgccg ctactcgaag
     3241 caggaaggct ttctgcctga cctggcggtg agcaagcagg tctatctgct cgatgccatc
     3301 ggcaaacttt tcgacttcta tagtctcgcc gatattgctt ttgtgggagg cacacttgct
     3361 cccatcggcg ggcacaatat catggaaccc tatgcctacg gtgtgcccgt ggtggtgggc
     3421 ccccatatcg agaagacccg ggatgtggct caggctctca gtcaggccgg cgctttgacc
     3481 atggtcagca gcggcgaagc cctcaccccg gccctttctc tcttgctctc cgacgaaagc
     3541 gagcggctca gccgcggcga gcgggggcgc ggcattctta tcgccagtca gggggccgta
     3601 ggcaaggcca tgcatatgct ggaagcgact ctttcaaaaa acaacccctc ccatgagctt
     3661 tcgggtttga actaatgacg acggcggtac aggccttgct tgcccccctg gccgggctct
     3721 atggtctggg ggcttatgcg cgcttgctca gttacagtct tggcataaaa agtagaaaga
     3781 aggccgatgc tttcgttctc agtgtgggca acctgaccgt gggcggcacc ggtaaatcgc
     3841 cggtggttat agatatagcc gccaggctga gcagacgagg cttgaaggtg gccgttcttt
     3901 cacggggcta cgggcgtcga ggtaagacac cgtatgtggt ggtatcagat ggtagcggta
     3961 agccccgttg cagtgtggaa gaaagtgggg atgagcctta cttgatcgct actgccgtgc
     4021 cggccgcctg cgtgattgtg ggagccagcc gggtgcaaac ccaggctctg gccatacaaa
     4081 aatacggagc cgaggttttg attctcgatg acggctttca gcacctgcct atggaacgcg
     4141 atatggatct ggtgctctgg gattacaacg atgaccccgg aacggctaaa ctcttaccgg
     4201 ccggtaggct gcgtgagccc ttttccgcct tgagccgggc cagtcatatt gtggtcacca
     4261 aggtgcctga ggttccagat caggcttatc tcgagggctt gctcaagacc ttgcgaagct
     4321 ataatcctga ggcggaaatg agcacggtca gcttccttat agactgcgtc gtcaatgccc
     4381 tggatctcgg gcgtagtttg ccgccctccg aactttccgg caagcgctgg ctggctctaa
     4441 gcgccatcgc cagaccggag agttttcagg cttccctgag gcagttggct ttggacgtgg
     4501 tggaggtgat gaccagacct gaccatagtt ggttttccga aagggacatc aggcaactga
     4561 gcgcggctct ggagtctggt agcgtggatg g
//
LOCUS       DAOQNZ010000113         4586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8144_length_4586_cov_3.182079, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000113 DAOQNZ010000000
VERSION     DAOQNZ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8144_length_4586_cov_3.182079"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKW73_03070"
     CDS             <1..211
                     /locus_tag="PKW73_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72289.1"
                     /translation="LTGKPNALEDFKQGGDDLQQKYSHYVWEAQALLELYPFQSIPSP
                     SNPRWDAQASAVLYTAIRLAEGKGD"
     gene            443..853
                     /locus_tag="PKW73_03075"
     CDS             443..853
                     /locus_tag="PKW73_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72290.1"
                     /translation="MTDREVSEIEWKAEEYDCVQMYLDDLDAPKEIGSIKLSLVGRIA
                     ALTTGLKQKEPPHRAGWWWFIPEAESCCPFILEVRATYTGFGSGVGAQYLSVQLPNER
                     MPVLQFLDSRKELHGVELEFYGPLPEPKWRQRGR"
     gene            998..1336
                     /locus_tag="PKW73_03080"
     CDS             998..1336
                     /locus_tag="PKW73_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72291.1"
                     /translation="MNSQAKKPRPRRGQKIFYRLSEDTYAIWFPYYQVTHNTINLHLP
                     PALTLRCHREGLVVMFVSETPEEARLRRCDWIKMQEAFPQEIKLLKTMVRDLKEYTLV
                     RLCQPEKAVL"
     gene            1344..1829
                     /locus_tag="PKW73_03085"
     CDS             1344..1829
                     /locus_tag="PKW73_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72292.1"
                     /translation="MSNPVRRIPNSLSSRVLVQLLLTPGLNTAALAEKMGFTGNLKCL
                     QVTLSRLRTQGELATVNRMHFAMKVEPDSAMDRSRLENIRTEAEFWRSKARVRAKQVE
                     HACRWIAGQAGKTVSPENANEVLRAISQIETGGKISGRAATRWLAEARQGTPWDTREE
                     V"
     gene            2037..2255
                     /locus_tag="PKW73_03090"
     CDS             2037..2255
                     /locus_tag="PKW73_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72293.1"
                     /translation="MSNDTRKCTVITLGDEAVEFAKAKLKVALDSLYEKGVISKREWK
                     YRLEAAGLMPRPQPAYYGSDRASKKESA"
     gene            2516..3586
                     /locus_tag="PKW73_03095"
     CDS             2516..3586
                     /locus_tag="PKW73_03095"
                     /inference="COORDINATES: protein motif:HMM:NF025599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RRXRR domain-containing protein"
                     /protein_id="HNA72294.1"
                     /translation="MSSHSVFVVSLEGKPVTPTTPAKARKLLRGGVAQPVWSKFGTFG
                     IRLLVPTRPETPVTTLGVDQGTKFEGYAVVCGRENPLSLKVDLPDKKKIVKKVRERAT
                     LRRARRFRKCWRRPARFDNRKRDAFLAPSQGVVVASRLKVLKALLGIFPVTLVGFEDV
                     RFHHAKYKWGANFSTVEIGKARIKAFLEAAGTNVSLYRGFETQELRQQYGYRKTSDKG
                     ADRFEAHCSDALTLACAVSLGEPVPPGLFLVVDDTYRAVRRRLHDTQPAPGGVRSAYS
                     RGTVFGVRKGLLIGAANGKTGQLCGEYQGGYRYTDALGKRQSTRSLQWVSTHFRLKPQ
                     TLVAGGNHKDGVSPSIQTRPKR"
     gene            3679..3978
                     /locus_tag="PKW73_03100"
     CDS             3679..3978
                     /locus_tag="PKW73_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72295.1"
                     /translation="MGKLIPLTEHIWVRDTAIVGCVPTFTNGRLAMLSIYVNGMGAIR
                     LSGSDAARFIDDQWSQSEEVEVHRSRFRSRESEEITLTPEREITNEDLEDDAEPF"
     gene            4058..4534
                     /locus_tag="PKW73_03105"
     CDS             4058..4534
                     /locus_tag="PKW73_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72296.1"
                     /translation="MLPVTAEDYLRVLTNAGALADHADSKKALAACKGMAEKLPKYGI
                     NANAMHLGMFLAQVSHESGGFAHYTENLNYTTLDRLFAIFPKALMLKGYNRGNASKLL
                     RNPKELAEVVYGGRKDLGNTEPGDGARFLGRGLIQITGRYNYTRAGEDIGIDLLAN"
BASE COUNT         1153 a         1196 c         1365 g          872 t
ORIGIN      
        1 tttaacaggt aaaccaaacg ccttggaaga tttcaagcag ggcggtgacg atttacagca
       61 aaaatacagc cattatgtgt gggaagctca ggccctactt gagctttacc cgttccaatc
      121 aattccaagc ccaagtaacc caaggtggga tgctcaagcc tctgcggttc tttacactgc
      181 aatcaggcta gccgaaggga agggagacta atgaagatca tcgagacaaa agaatggagt
      241 atccttgtgg cctgccctca ctgccaatca aggctggccg tcgaggccgg tgacgtgagg
      301 tattgctcgc atatcgacta cagaggcgat aaggattact ggtacgcagc caactgcgcg
      361 gtgtgccagg gatcaatagc cctgccaaga acgaatatcc cagctcatat ctgccgtatg
      421 gcagaagcaa ggagcgttga aaatgaccga tagagaagtc tccgagatag agtggaaagc
      481 tgaagagtat gattgcgtcc agatgtatct tgatgatctt gacgccccaa aagaaatcgg
      541 aagcataaaa ctttccctcg tgggaagaat tgcggcttta acaaccggac tcaaacaaaa
      601 agagccacca cacagagctg gctggtggtg gtttatccct gaagccgaaa gttgctgtcc
      661 ttttattctg gaagtccgtg caacgtatac gggatttggc agcggagttg gggcgcagta
      721 tttaagcgtg cagcttccca atgagaggat gccggttctt cagttcctgg atagccggaa
      781 agagctgcac ggggtagagc ttgagtttta cgggccattg ccagagccaa aatggagaca
      841 gcgaggccga tgatgaagct taaggcagga gatgactggt atgaagtaga tgagcaggag
      901 cttccatcgg tctacaaaaa agtgatcttc ccggttgacg aggaccggat taggcaggcc
      961 atcagggcca actacttacc aaaaggagtt aggaaaattg aacagccaag ccaagaagcc
     1021 acgaccacgc cgaggccaga agatatttta ccggctcagt gaggacacct acgccatctg
     1081 gttcccctac taccaagtca ctcacaacac catcaatctc catctccctc ccgcccttac
     1141 gctgcgctgt caccgggaag gactcgttgt catgtttgtc tcggagacac cggaagaggc
     1201 acggcttcgg cgctgtgact ggatcaaaat gcaggaggcg ttcccgcagg agatcaaact
     1261 gctcaagacc atggtgcgcg acttaaaaga atacacgctc gttagacttt gccagccgga
     1321 gaaagctgtg ttataaatat aacatgagca atccggtccg ccgtattccc aactcgctct
     1381 caagccgggt tctggtccag ctcctgctga cgccgggcct gaacacggct gcccttgcgg
     1441 aaaagatggg cttcactggg aacctgaaat gtcttcaggt gacactctcg cgcttgcgaa
     1501 cacaagggga actggcaact gtcaaccgga tgcactttgc tatgaaggtg gaacctgaca
     1561 gtgcgatgga ccgaagccga cttgaaaata ttcgtacaga ggcagaattc tggcggagca
     1621 aagctcgcgt ccgggccaag caagtcgagc acgcctgccg gtggatcgcc ggacaggcag
     1681 ggaaaacagt cagccctgaa aacgccaacg aagtcctccg cgccatctct caaatcgaaa
     1741 ctggaggaaa gatttctggg cgcgctgcta ctcgctggct tgccgaagcc cgtcagggga
     1801 caccctggga taccagggag gaagtatgag tttgactttg catggccggg ctacatggtg
     1861 gcggttgaga tcgagggcgg gatcggatgg gaagctgacg gcacggtcaa aaagtcgcgg
     1921 cactccatgc cggaagggta ctccgaggat tgtcgcaagt acaatctggg ccagctccac
     1981 ggatggattg tgctgcggtt caccacgctc atgctgaaag aacaggagat tggcgcatgt
     2041 ctaacgatac tagaaaatgc actgtcatta cgctcggcga tgaagccgta gagtttgcca
     2101 aagcaaagct gaaggtggcc cttgacagcc tttacgagaa aggcgtgatt tcaaaaaggg
     2161 aatggaagta tcgacttgag gcggcgggcc tgatgccacg tccccagcca gcttactacg
     2221 gctccgaccg agccagcaag aaggaatcag catgaccaag agctatgatg agtcagcgac
     2281 tccacctaaa ccaaaaaacg gttataggtg gagcttgtaa tgagggtagc aacgcaagcg
     2341 ttgactagag ggctgggagc ccaagctccc agcagcagcc ttcccggcta caagtaccct
     2401 ggagtgctcc acgagcccca ggccctacgg cagaaccctt gaatgccacg tctcgcagtt
     2461 tgagacgaag ccaggacggc aatctcgacg tggaatcact ctgaaaggag aacgtatgtc
     2521 gtcacattca gtttttgtgg tcagtcttga gggaaaacca gtcacgccga ccaccccggc
     2581 caaggcgcgc aagctgctgc ggggcggggt ggcccaaccg gtctggtcca aattcggaac
     2641 cttcggcatc cgcctgctgg tcccgacccg gccggaaacc ccggtgacca cgttgggggt
     2701 ggaccagggc acgaagtttg agggatatgc ggttgtctgt gggagggaga atccgctttc
     2761 gctgaaggtg gatttgccgg acaaaaagaa gatcgtcaaa aaggtcaggg aacgggcaac
     2821 cctgcgccgg gcacggcggt ttcggaagtg ctggcgcaga ccggcgcggt tcgacaaccg
     2881 gaaacgggac gcctttctcg ctcccagcca gggggtggtg gtggcttccc ggttgaaggt
     2941 gctgaaggcg ttgctgggaa tatttcccgt gaccttggtt ggttttgagg atgtccgctt
     3001 tcaccacgcc aaatataagt ggggggccaa cttctcaacg gttgagattg gcaaggcccg
     3061 catcaaagcg tttttggaag cggcgggcac caatgtttcg ttgtatcggg gttttgaaac
     3121 gcaggaactg cgccagcagt acggctaccg caagacctcc gacaaagggg ccgaccggtt
     3181 tgaagctcat tgttccgatg ccttgaccct ggcctgtgcg gtgagtttgg gagaaccagt
     3241 cccgcccgga ttgtttctgg tggtggacga cacctaccgc gccgtccggc ggcggttgca
     3301 tgacacgcaa ccggccccag gcggagtgcg gagcgcctat tcacggggca cggtgtttgg
     3361 ggtccggaaa ggactcctga ttggtgccgc caacggaaaa accggtcaac tgtgcgggga
     3421 atatcagggc ggttaccggt acaccgatgc gctgggcaaa cgacagtcaa ctaggtcgct
     3481 gcagtgggta agcacgcact tccgcctgaa accacaaacc ctggttgccg gtggcaacca
     3541 caaggacggc gtttccccat ccatccaaac gcgcccaaaa cggtaacggt gcagcaattc
     3601 gcaacatgcg agtgattcca atagccgaga agggcaggat taaaatgatc aaccagatca
     3661 caggagcggt gattgagcat gggtaaattg attccattga ccgagcatat ctgggtgcgg
     3721 gatacagcca tcgtcggctg cgtccccacc tttacaaatg gcaggctggc catgctgagc
     3781 atatacgtga acggaatggg agcgatcagg ctttccggca gtgacgccgc ccgctttatc
     3841 gatgaccagt ggagccaaag tgaggaggtg gaggtgcatc gcagccgctt ccgcagccgt
     3901 gaatcggaag agattacgct cacaccggaa agagagatta cgaatgaaga ccttgaggac
     3961 gacgccgagc ctttttgaca cagctcccga agtaaagaaa ggggaggcgc tccctgaagc
     4021 caagcaaaaa attgcagccc cacggaaagg atcgaatatg ctgccagtca cagcggagga
     4081 ttatctgcga gttcttacca atgccggagc gttggccgat cacgccgaca gcaagaaagc
     4141 cctggctgcc tgcaaaggaa tggcagagaa gctgcccaag tacggcatca acgccaacgc
     4201 tatgcacctg gggatgtttc tggctcaggt gagccatgag tccggcggct tcgcccatta
     4261 caccgagaac ctgaattaca ctacgcttga ccggctcttc gccatcttcc ccaaggctct
     4321 gatgctcaaa ggctacaacc gagggaatgc gagtaagctc ctgcggaatc caaaagagct
     4381 ggccgaggta gtatatggcg ggaggaagga cctgggcaac acagagccag gggacggcgc
     4441 tcgattcctg ggccggggcc tgattcagat caccgggcgc tacaactaca cgcgagccgg
     4501 agaggacatc gggattgacc ttctggcaaa ttgaaggtgc tgcaggccga ggacgagatc
     4561 gccggcatct gctcggccat cggcgc
//
LOCUS       DAOQNZ010000114         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8194_length_4568_cov_3.847552, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000114 DAOQNZ010000000
VERSION     DAOQNZ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8194_length_4568_cov_3.847552"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PKW73_03110"
     CDS             complement(<1..868)
                     /locus_tag="PKW73_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNA72297.1"
                     /translation="MENKEDEKNSKYGTAPLTESAVEPADDDRPEVAVHSPQDEDGAP
                     AYAAEASEGVLSAEYEALKLELQRLRRKESELLARQSQAKQKYRLEHPPRSCVAGLHS
                     LSPQQRLLLLKDSAAQMEYVMHSATIPAFKVSEGELIVEANQAFFSYLGLEPEAVYGS
                     KLTLKHVSTPESIALERAHLADFKETLVTRPFETERVSKDGWLKPVMTTLRVTRLEPL
                     EYVAFWLDLTELRYLEDSIKQRNSIFSAIVEEMPHIVFVCNQDGAARQFNRRLYELTG
                     TNGSRDDGYLLEH"
     gene            complement(1179..1877)
                     /locus_tag="PKW73_03115"
     CDS             complement(1179..1877)
                     /locus_tag="PKW73_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNA72298.1"
                     /translation="MTTLNDKLKQVLSIQDESECAKSFEKFCQENFHDLPLPGENSMF
                     DRAMWMASQFLSLSKSDEEVVKRVTNRMEAKLPREVSALDQAVDKAVFHLKDLLGQAR
                     ETAIGTWQDMLSACAWQQMVPAGATRGVGSQLVSLGTFEKIEGDVRIQVNLGWMVDQN
                     NLRLLLQAKDSEDNAIADVEVRVTESDKGLIYSSKTNQDGAVVAPAVKINPGQYQIQV
                     FWSDKVVETPFFRV"
     gene            complement(1893..2744)
                     /locus_tag="PKW73_03120"
     CDS             complement(1893..2744)
                     /locus_tag="PKW73_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72299.1"
                     /translation="MNNKESRAFLKGEMEFTPQRMMRYIRSRARLATSEDIEDILQNA
                     LVLVTQHFDPGHPDAKLALYCMGACNKAIAQNLERYHKISLKKRGDSGKEAKDGAPAT
                     ADEGEGEGQEGSDLGESPVGLPKTAGIAQSAVGPADLRQAELDGAVVTVKTLSPGKSF
                     DETPSTTSQYRTTSLDTMFDDDDGRSADDLFGGDMNVLASGGGGSDPAGRALINDLVE
                     LVLAELPSQLHRTCFVLKYVEGYKREDLIECLKMDAKSIDTIDKKIVRTLKAFKDKLD
                     QDEARIR"
     gene            3134..>4568
                     /locus_tag="PKW73_03125"
     CDS             3134..>4568
                     /locus_tag="PKW73_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA72300.1"
                     /translation="MNNNFSSTDQNKYSPQTPAPVAPADQNMVTTGSSQTLILPNPKD
                     TGSQSAPDKSAAVNDSTKVLGTVQFNEATPRPESGPGTTILQSLDEALVKSPRAAAIR
                     AQLNITRSAFAEAAAQPNAGLFFDRGPYAEQVRRIGPSFSWEPPWKLVFRLLATKRLV
                     DQARTDLMAALWTLRADVRRAYTEAVVAQETVETLNSLYDLASKLSQVSDKRFQAGDV
                     PELDVLKARLATSQADVERQVGLRRVIRAKQQLNIIMGRSPETPINIPRLPAYLKDQS
                     KVKLDESQKNQLLPDFDQDMPPLNVFIGKALEQRLELQSIAQRLKLNDANLRTAYGNV
                     IPNPSVSFGSSVSGNAPTGPKLSSIYVSLNIETPLTNWNQGEIFRLKAVGKQLKYEMD
                     SQKNIISQQVSSAYNNVLAARQKLKVYQDHVLADSYEVARLSRRSYEVGQSDITATLQ
                     VQQANINIRGQYLDAVRLYQDAYTELEQ"
BASE COUNT         1088 a         1321 c         1139 g         1020 t
ORIGIN      
        1 aatgctcaag caaatagccg tcgtcgcggc tgccattggt gccggtcaat tcgtataggc
       61 gccgattaaa ctggcgggcg gcgccgtctt gattgcaaac gaagactata tggggcatct
      121 cttcgacaat cgccgagaat atggagttgc gctgcttgat actgtcttcc aggtacctga
      181 gctcggtgag atcaagccaa aacgccacat actccagagg ctcaaggcgg gtcacccgca
      241 gagtggtcat gacaggtttc aaccaaccat ccttgctgac gcgctctgtc tcaaagggac
      301 gggtgacaag ggtttctttg aaatcagcca ggtgcgcacg ctccagcgca atagactccg
      361 gcgtggacac atgcttgaga gtcaacttgg agccgtatac ggcttccggc tccaagccca
      421 ggtaactgaa aaaggcctga ttggcctcga cgataagttc gccctcggac actttgaagg
      481 ccggaatcgt agcggaatgc atcacatatt ccatctgtgc ggcactgtcc ttgaggagca
      541 ggagccgctg ctggggcgag aggctgtgta aacccgccac gcaagaccga ggcgggtgct
      601 caaggcgata cttctgctta gcctgggatt gccgggctag cagctccgat tccttccgcc
      661 tcaagcgctg gagttccagc ttgagggctt cgtattcagc gctcaagacc ccctccgacg
      721 cctctgctgc ataggcagga gcgccgtctt catcctgggg agagtgcacc gcaacctctg
      781 gacgatcatc atcggccggt tccacagcag actcagtcag cggcgctgtc ccatacttgc
      841 tattcttctc gtcttcttta ttttccatag ggccaatcga atgaacgagc cgaacgaatg
      901 caccaaatga acgctccaaa ggtacgcctc aattcattct gtctggattt tgccaacacc
      961 tgggaagaga ataacacaaa acttccaacc tcttgccaac cttgcgtaat atgaacggtt
     1021 tttcgccctt cccaaaatta gaaagaggca ccaggtgggt gcctcttcga tagatttcag
     1081 agatttttca ggtctgcggg gacaagaaac cgctgcctga tgctttcttt gccggaaccg
     1141 aaaggcgcca gtggcgcagt cctcggtctg gagcgttgtt agacccggaa gaagggcgtt
     1201 tccaccactt tgtcggacca aaacacctga atttgatact gacccggatt gatcttgacc
     1261 gccggtgcca cgacggcacc atcctggttg gtcttcgaag agtagataag acccttatcg
     1321 ctttccgtta ctctcacttc cacatcggca atggcattgt cctcgctgtc tttagcttgc
     1381 agaagcaggc gcagattgtt ctgatcgacc atccaaccta agttcacttg aatgcgaacg
     1441 tcaccctcaa ttttctcgaa agtaccaaga gataccaact ggctccccac cccgcgggtg
     1501 gctccggccg gtaccatttg ctgccaggcg caggctgaga gcatgtcctg ccaggtgccg
     1561 atggcagttt cgcgagcctg accgagcaag tccttgaggt gaaaaacagc tttatccaca
     1621 gcctgatcca gggctgagac ttcccggggg agcttcgctt ccatacgatt ggtgacccgt
     1681 ttcaccacct cttcatcgga tttagaaaga gaaagaaact gggaagccat ccacatagcc
     1741 cggtcgaaca tggaattttc cccgggcagg ggcaagtcgt ggaagttttc ttgacagaac
     1801 ttctcaaacg acttggcaca ctcgctttcg tcctgaatcg agagcacttg tttaagtttg
     1861 tcatttagcg ttgtcattgc catctcctga atctatctaa ttcgtgcctc gtcctgatct
     1921 agcttgtctt tgaatgcctt cagggttcgg acgattttct tgtcgatggt atctatactc
     1981 ttcgcatcca tttttagaca ttcaatcaaa tcttctcgct tataaccttc cacatacttc
     2041 aaaacgaagc aggtgcggtg caactgggat ggcaattcgg ccaggaccaa ctccaccaga
     2101 tcattaatta atgctctacc ggccggatcg ctgccgccgc cgccggaagc aagcacattc
     2161 atatcgccgc cgaaaagatc atcggcactg cgcccgtcat catcatcaaa catggtatcg
     2221 agagatgtgg tgcggtactg actggtggtg gagggtgttt cgtcaaagga ttttccaggc
     2281 gagagcgtct tcacagtcac cactgcgcca tccagttccg cctgccgcaa gtcggccggg
     2341 cccaccgcgc tttgagctat accggcggtt ttgggcagtc ccaccggcga ctctcccagg
     2401 tcgctgcctt cctgcccctc accttcaccc tcatccgctg tagccggggc gccatcctta
     2461 gcctctttgc cagaatcgcc gcgcttcttg agggaaattt tgtggtatcg ctccagattc
     2521 tgagcaatcg ctttattgca agcacccata caatataaag ctaacttggc atcgggatgg
     2581 ccgggatcaa agtgctgagt gacaaggacg agtgcattct gcaatatatc ctcgatatct
     2641 tcactggtag caagccgtgc ccgagaacga atgtagcgca tcattcgctg gggagtgaac
     2701 tccatctccc ctttcaaaaa tgctcgactt tccttgttgt tcattctcta atgactttca
     2761 caatgtttac cggcggaccc tgcaaccagg acctttcatg acaactgaaa agtaccgccg
     2821 ggcgggtctt caagcagtgc gcaagcacct aagggctgca atgttagcag attataagag
     2881 gaagcaggaa gacgcgaaac cgttatactt gagccggcgg aaacagcaac agctataaat
     2941 gactgcttta gtaaatcgga atccattgtc tatagtcctc aataaatctt ctgggcttat
     3001 agtttctgtc acgatggcga ctgtgctctc acactcagcc atcgctgaag gtaagtcgcc
     3061 ttcaaacctg tatgtacccg caaccgactc cattctggac aaattggccc agaatccggc
     3121 cttgcggccg gccatgaaca ataacttttc ctcgactgat caaaataagt attcacctca
     3181 gacgcccgcc ccggtagctc cggccgacca gaacatggtc acaaccggct ccagccaaac
     3241 cctcattctc cccaatccca aggacaccgg cagccaaagc gcgccggaca aaagcgcagc
     3301 cgttaacgac tccaccaaag tgctgggaac ggtgcagttt aacgaagcca cccccaggcc
     3361 ggaaagcggc cccggcacca caatattgca atctctggac gaggcccttg tgaaaagccc
     3421 gagggcggca gccattcgcg cccagcttaa cattaccagg tcggcctttg cagaagccgc
     3481 cgcccaaccc aatgccggct tgttcttcga ccgcggtccc tatgccgaac aggttcgccg
     3541 aattgggcca tcctttagtt gggagccgcc gtggaagcta gtcttccgcc tgctggccac
     3601 taaacggctg gtagaccagg cccgtaccga ccttatggcc gccctctgga ccttgagggc
     3661 cgacgtgcgc cgggcctaca cggaagcagt agtggctcaa gagaccgttg aaaccctcaa
     3721 ctccctctat gacctggcca gcaaactctc ccaggtttcg gataaacgct tccaggcagg
     3781 cgacgtgccg gaattggatg tcttgaaggc aaggctggcc acctctcagg ccgatgtgga
     3841 aagacaggtg ggtctgaggc gggttataag agccaagcaa cagctcaaca tcattatggg
     3901 acgcagtccg gagaccccca taaacattcc caggctgccg gcttatctga aagaccagag
     3961 taaagtaaag ctggatgaga gtcagaagaa ccagctcttg cctgatttcg accaggacat
     4021 gccgcccttg aatgtcttca tcggcaaggc gctggagcaa cggctcgaac tacagagcat
     4081 tgcccaaagg ctcaaattga atgatgccaa cttgcgtacc gcctacggta acgtcattcc
     4141 caatccgtcc gttagtttcg gctcctcggt atcaggcaat gcccccaccg gtcccaagct
     4201 aagctctatc tatgtttccc tcaacattga aacccctctg accaactgga accagggcga
     4261 aatattccgc ctcaaggcag tgggcaaaca actcaagtac gagatggata gccagaaaaa
     4321 catcatcagc cagcaagtct catcggccta caacaatgta ctggccgcca gacagaagct
     4381 caaggtttat caggatcacg tgctggcgga ttcctatgaa gtagcccgcc tatcgaggcg
     4441 cagttacgaa gtaggacagt cggacattac tgcgacattg caggtgcaac aagccaacat
     4501 aaacattcgc gggcagtatc tcgatgccgt acgcctttat caggatgctt ataccgaact
     4561 ggaacagg
//
LOCUS       DAOQNZ010000115         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8224_length_4563_cov_2.915484, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000115 DAOQNZ010000000
VERSION     DAOQNZ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8224_length_4563_cov_2.915484"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PKW73_03130"
     CDS             complement(<1..263)
                     /locus_tag="PKW73_03130"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA72301.1"
                     /translation="MLPIADDNPTTSYPVINHLLIFFNLAAFVAQILFLADAPTVGKL
                     LSDDFSMVPARFLSQFSLVQFGTLFSSMFMHAGFSHIFGNLWYL"
     gene            369..1337
                     /locus_tag="PKW73_03135"
     CDS             369..1337
                     /locus_tag="PKW73_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNA72302.1"
                     /translation="MRYDLPLTVYFLCLFTLLGCFSRMAWPFDLMAQFRMHYVALSAC
                     LFAVVFYFGKTSPIRRRMLLLCLTSAALNTVAVYLGPIRPLGQASPRSFKLMQFNINV
                     SNQNKEGVLALIEREKPDLVCLQEVDQSWVETLESKNSKQMQVYPEKLLVARGDPFGM
                     ALLSKFKIKASQTLYFNEEKLPSLVASLELPGGADLNLLVTHPMPPLTPALYKSRNQH
                     LENLSRYISTLKGENTIVCGDFNSVGYADNLKAMAERNHLREPVSLLNLPCPAQPPGT
                     WPAIVPPPMRIPIDHVFMSKALTAQAVKTDDACDSDHVPLITIISY"
     gene            complement(1362..2159)
                     /locus_tag="PKW73_03140"
     CDS             complement(1362..2159)
                     /locus_tag="PKW73_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72303.1"
                     /translation="MNKSAEGKDYAAELVAGLKIASPCSMRFEDMKHTDETYKRFCGD
                     CSKNVFDVASMSREQVAQLVEESWRTDGTMPCLRLSRRTDGTVITDDCPGGLRRGRNF
                     YRRLKASAAAFAAFFLGGMPNLQARADEPLMGKPMAPGPRDTRPLMGAVCPPNWAQMA
                     SSRPEIKKIQDGLAALEKKSDKGSAADRAKLARLQLSLARAANSANFGYYASQVLEQA
                     AALARQNGDKALLSDILKEKLNTLDVLKVKDKSSVQAEIDALKKEGK"
     gene            2481..2855
                     /locus_tag="PKW73_03145"
     CDS             2481..2855
                     /locus_tag="PKW73_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNA72304.1"
                     /translation="MKTAILVYSDPKNGSEESLGRLFNALAATYDFKKNGDDVTLMFQ
                     GAGTRWVGQLSQPDHPAYELYEAVRDKVAGVSCGCADVFGASEDAEKEGYALIKDNSV
                     PGTSGLTSLRKLISDGYTVVNF"
     gene            2950..3876
                     /locus_tag="PKW73_03150"
     CDS             2950..3876
                     /locus_tag="PKW73_03150"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNA72305.1"
                     /translation="MDSEGPYHKGEIAIQEAVGERAQALMNSVVIKDEILTGAINFIE
                     RQPLMVLATENANGQIWASMLFGKPGFIRALSTKELLVDLLQAKEIDGDPFLENLASN
                     PRIGALLIELDTRRRLKINGRVSRQNEQEILIAVEESFPLCPKYIQRRKVRLPEDGLP
                     HKQTPELATGTSLGAEELELIANCDTMFSATSNPQGNLDISHRGGKPGFVRAIHATRL
                     RVPDFAGNSMFNSFGNLQLNDRAGVILLDFAGRQCLQICGRATVLLAQSDPQNETGGT
                     NRFWELEVENWQRITIPAIELEFLDASPHNPK"
     gene            3999..4541
                     /locus_tag="PKW73_03155"
     CDS             3999..4541
                     /locus_tag="PKW73_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA72306.1"
                     /translation="MDKSALVSELLELFRQHGYEGVSIGDVSKATGLGKSSLYHHFPG
                     GKEEMALEVLGHIDMAVKQYFIAPLKAEGAPEAKLKAMAKTIEDFYEGGRKGCIVDGL
                     TLGAASAPFQELIRNCLEAWIEAMAAVAKEHGVSEKLARERAENALIAIEGALVVSRA
                     LQNYQIFKRVTRNLPRLILD"
BASE COUNT         1200 a         1241 c         1135 g          987 t
ORIGIN      
        1 agataccaga gattgccaaa aatgtggctg aatcccgcgt gcatgaacat cgaactgaag
       61 agtgtgccaa actgcaccag cgagaactgg ctcagaaagc gagccggaac catggagaaa
      121 tcatcgctca ataatttgcc gacggtggga gcatcagcca ggaaaagaat ctgcgcaaca
      181 aaggctgcca ggttgaagaa gatgagcagg tggttgatga ccggataact agtggtcgga
      241 ttgtcgtcgg caataggcag cattatcggt tcctttcata ctggctgtta ttccccgcca
      301 gagcaacatc acaccaggcc ggtcggttgg ctataatgcc ccaggtgctt tgaaattcag
      361 acaaatacat gcgatacgac ctgccactca ccgtctactt cctctgcctt ttcaccctgc
      421 tgggctgttt ttcccgcatg gcctggcctt tcgatctaat ggcccagttt cgcatgcact
      481 atgtggccct ttcggcgtgc cttttcgccg tcgtgttcta cttcggcaaa acttccccca
      541 ttagaaggcg catgctgcta ctctgcctga caagcgccgc actaaataca gtggcagtct
      601 acctgggacc gattcgcccc ctggggcagg ctagcccccg cagtttcaag ctcatgcagt
      661 tcaatataaa cgtctccaac cagaacaaag aaggcgtact ggccctgatt gaaagagaaa
      721 agcccgacct agtttgtctg caggaagtag accagagctg ggtagaaacc cttgagagca
      781 aaaactccaa acaaatgcag gtctatcccg aaaaactcct ggtggccagg ggcgatccct
      841 tcggcatggc acttctcagc aagttcaaaa tcaaagcgtc ccaaaccctc tatttcaacg
      901 aagaaaaact acccagcctg gttgccagcc tggaactgcc gggaggcgcg gacttaaacc
      961 ttttggttac acatccgatg ccacccctga caccagcgct gtataaaagc cgcaaccagc
     1021 atctggaaaa tctctcccgc tacatttcta ctctaaaagg agagaacact atagtttgcg
     1081 gcgacttcaa cagtgtgggc tatgccgaca atctgaaagc aatggcagag aggaaccacc
     1141 ttcgggagcc ggtttctctc ttaaatctac cctgccccgc acaaccaccg ggaacctggc
     1201 cggcaatagt tccaccgcct atgagaattc ccatcgatca tgttttcatg tcgaaagcac
     1261 tgacggcgca ggcagtaaaa actgatgacg cctgcgactc ggaccatgtg ccgctgatca
     1321 cgatcattag ttattagtat ctgcgcgaaa gagaaccagt gctacttgcc ctcttttttc
     1381 aaggcatcaa tttcggcttg cacactgctc ttatctttca ccttgagcac atcaagggta
     1441 ttgagttttt ccttcaagat atcggacagt agcgccttgt cgccgttttg cctggcaagt
     1501 gccgccgctt gctccaggac ctgggaagca tagtatccaa aattggcgct gtttgccgcc
     1561 cgggccaggc tcaactggag acgggccaac ttggcacggt cggcggcgct acctttatca
     1621 ctctttttct caagagcagc cagtccatct tgaattttct tgatttcagg gcggctggat
     1681 gccatctgag cccaattggg aggacaaacg gctcccatca agggccgcgt atctcgcggc
     1741 cccggcgcca taggtttgcc catgagaggc tcatcggccc gggcctgcaa gttcggcatg
     1801 ccgcccagga agaaggccgc aaaggcagcg gcagaggctt tcagacggcg gtagaagttt
     1861 ctcccccggc gcaagccccc cggacaatca tctgtaataa cggtgccgtc ggtacggcgg
     1921 gacaaacgca aacatggcat agtgccgtct gtccgccagc tttcttccac aagttgtgcc
     1981 acctgctccc ggctcatgga agcaacatca aaaacgtttt tggagcaatc accgcaaaaa
     2041 cgtttatagg tttcatcagt atgcttcatg tcttcaaagc gcatggaaca aggactggca
     2101 atcttcaagc cggccacaag ctcggcggcg tagtctttgc cctctgcgct cttattcatc
     2161 tgcatatagt ttgcctcgcc atcaaccatc aacctgatgc taacagagta cacccggaaa
     2221 ttgcctgggt aaaaccctga gaaccaactg gtgaaagtgg gcgctttctt gtgcttgccg
     2281 ccgttggtaa aacagtcggc tcctatagcc aagggacgag cctcgaatct ggaccgaaaa
     2341 tgcccaagcc ccaccggcta ggcatctccg gacttcgtca attcaggggt tgacattgta
     2401 ccgatcgttc ggtacagtaa ttacatgaac ggttcaatcg attgaaagac gaaccagata
     2461 acatttaagg agctacgaca atgaaaaccg caatcctcgt ttattccgac cccaaaaatg
     2521 gcagcgaaga atcccttggt cgcttgttca acgccctcgc cgccacctat gatttcaaga
     2581 aaaacggcga cgatgtcacc ctcatgtttc aaggtgccgg cacacgctgg gtcggacagc
     2641 tttcacaacc ggaccatccg gcttacgaac tttacgaagc tgttcgcgac aaggttgccg
     2701 gtgtatcctg tggttgtgca gatgttttcg gcgcttcgga agacgcagaa aaagagggtt
     2761 acgcactaat caaagacaac tccgttcccg gcacatcggg tctgaccagc ttgcgtaagc
     2821 tgattagcga cggatacacg gtagtaaact tctaaaagga ctaacagtcg gttgtttcac
     2881 ctgagagaga ggcggctcgt tgaattcagc gacgagccgc ctccctgaca ccagcaaagg
     2941 agacgagaca tggatagcga aggtccttat cataaaggtg aaatagccat tcaggaggcc
     3001 gtgggagaac gggctcaggc actgatgaat tcagtggtta tcaaagatga aatcttgact
     3061 ggtgccatca acttcataga acgccagccg ctaatggttc tagccacaga aaatgccaat
     3121 ggacagatct gggcttcaat gctctttgga aaaccaggat tcatcagggc actaagtaca
     3181 aaagaactgc tagtggactt actccaggcg aaggaaatcg acggagatcc ttttcttgaa
     3241 aatctcgcaa gcaacccgcg cattggcgcc ttgttgattg agctggatac tagaagaaga
     3301 ctgaaaatca acggaagagt ttcaagacaa aacgagcaag aaatcttgat cgcggtagaa
     3361 gaatcctttc ccctctgccc caaatatatc cagcgccgta aagtcagact gccggaagac
     3421 ggactgcctc acaagcagac tcctgaactg gcaacgggta caagtcttgg cgccgaggaa
     3481 ttagagctaa tcgcaaactg tgacaccatg ttctctgcaa cttcgaatcc gcaaggtaac
     3541 ctcgacattt ctcaccgcgg cggcaaacct ggctttgtgc gggcaatcca cgccaccagg
     3601 ctgcgcgtac cggattttgc cggtaatagc atgttcaatt ccttcggcaa tctacagttg
     3661 aacgacagag ccggcgtcat cttgctcgac ttcgcaggta gacaatgcct gcaaatatgc
     3721 ggaagagcaa ccgtacttct agcccagtca gacccgcaaa atgaaaccgg cggcaccaat
     3781 cgtttctggg agctggaagt agagaattgg caaagaataa ctattccagc cattgaactg
     3841 gagtttctgg atgcctcgcc ccacaatcca aagtaaccgc atcaaaagtg gtgccttcag
     3901 ctaaggcacc agacccttcc aaagccagag agggagtctg tcaaagattg acgagcggct
     3961 caatgttcgg taacctttcg aacgcaagga gctgaatcat ggacaagagc gcactagtat
     4021 ctgaactgtt ggagttgttt cgccagcacg gctacgaagg cgtctccatt ggcgacgtgt
     4081 ccaaggccac cggtttaggg aaatcaagcc tttaccatca ttttcccggc ggcaaggaag
     4141 aaatggcgct tgaagtgctt ggacacatcg acatggccgt caaacaatat ttcattgcac
     4201 cactcaaggc agagggtgca ccggaagcca agctcaaggc aatggccaag accatagaag
     4261 acttctacga aggtggccgt aaaggctgca ttgtagacgg actaacgctt ggtgccgcca
     4321 gtgcaccatt tcaagaacta atccgaaact gccttgaagc ctggatcgag gccatggctg
     4381 ccgtggccaa agaacacgga gttagcgaaa aactagcgcg agaacgtgcg gaaaatgcac
     4441 tcattgctat cgaaggcgcc cttgtggtct cccgcgccct gcaaaattac cagatattca
     4501 aacgcgtaac aagaaatctc ccgcgcctga ttttagatta gtgtccgcac cgggtcgacg
     4561 gct
//
LOCUS       DAOQNZ010000116         4561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8232_length_4561_cov_3.559254, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000116 DAOQNZ010000000
VERSION     DAOQNZ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8232_length_4561_cov_3.559254"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2329
                     /locus_tag="PKW73_03160"
     CDS             <1..2329
                     /locus_tag="PKW73_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72307.1"
                     /translation="PEPSGASEGSIGGETAAPPPAPPPAHVPPPPPQSTIGAGPQTGM
                     APPSVGVSPQTGSAPQAVGVSTQVAACAIAMPEPIPCAVPPVEIKASTRGDEVELLER
                     TRLSGDQDLHLTGSLTALQLGNMEAIIRANRDKSVKIAWCAAPRLEGGKVVKRPLKPA
                     VYEGVSRLYVVVDGSSVMSKYLKSVAAGLKSLPSSINSTIYLVGDQSLRFGNSGFKGD
                     EKPYLEAVQKVEGFVAVGGQDDSLVLSEALHRAGKEKDSAVLWIHGAQPLSPGSFTPI
                     QRMLVGSPLRPVLFDLPVSSGPNEILNGVDADTRILRVNRSGDLADDLKNLFHSIDPS
                     FAASREQLAAQQLQYVDSSGLTEAGARKVSPWLVNIFAAAEIKRKMSGCPTARISESP
                     ALLADLCQIVSPVSSAIVVSPDSHAYVAKTETEDRFFQSVGEFVSRIFLGSINAVGPL
                     REVAPRKYYYELPASSPAPPPPVFQGQNPIDSAFNGVTRQLNSLSSAAGAPTGSYGAA
                     IQSKAAKQSMSAGKFDQAPARSDAFGGGGDAFGGGSDAFSGGGGAYDGAKGNAAGVSG
                     APIGGDARRMNEQTYGASDGPAAGEPLGDAYGSSIPVDKTRALEELPASVPAQAPPPP
                     SPQGFTTAKDELSMAAPVAEKKSVIDAERDSMEGADLSRLKEAQASGKGQRFDFQASR
                     YRLESGPVFQGATNGTILPQFQNFGQDDAGKNAGRVGAPVHSRNRQYNRLGAEEYNEE
                     ASLQKTANLFTLIVVVAGLVIWFLFYRGRNKEEDV"
     gene            complement(2326..3696)
                     /locus_tag="PKW73_03165"
     CDS             complement(2326..3696)
                     /locus_tag="PKW73_03165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA72308.1"
                     /translation="MDLETARFVLSKSGEDCLKELTARYPSIAAPQELSAIEWLRKHA
                     RLNQAQSSAILEVAKARQRAAETKKFSRAAQMFFTREALEQASGEEIAAYRSRRFARV
                     FEANTAIADLCCGIGGDTTALSRHFSVTGVDLDQARLIFAAANAAVYGNAERFAGLHE
                     DVKECKISSYQGAFFDPGRRTKDGRRISNPEHYLPPLSTIHSWLPHLKGLAVKLSPGI
                     DYEKLKDLDCEIEIISQDGEVKEALLWFGTLKEVGGETSGKVARHRATLLTSQKEKAP
                     TRYKDERAPDENDRAEVDSNSAPSDQDKQIITLSDADGMEPLAVAPLARYLHEPDGAI
                     IRAGLVELVGARFNALKIDQDIAYLTGATPAKAPLMRSFEVLAAMPFSLKKLKAELQA
                     LACTSVVVKKRGSPIAPEELLKALALKNAHKKAKHDAEPQTELTVFLTHLNGIHSAII
                     TRETKA"
     gene            complement(3753..>4561)
                     /locus_tag="PKW73_03170"
     CDS             complement(3753..>4561)
                     /locus_tag="PKW73_03170"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA72309.1"
                     /translation="GTKPGTGATFNPQVANSIGQKYALVIGISNFEDPSLNLKYSAKD
                     ARDFCSYLTGPGNFKQENTRLLIDKEASLENIVDLIGDSWLPRVVKPDDLVVIYFSGH
                     GSPAEMDVKGDNYLVVYNTKPNHLFATGLSMTKLLPMIKERVKTERVLLVFDACHSGA
                     AREGAKGLYRADNFDSQALAQGTGQMVICSSAPDQVSWESKQYPNGVFTRQLIDILAA
                     DGGTTPLSKIFLQLKDNVQAEVLRDRGRVQTPEFKSAWNGNDLKLNIVTK"
BASE COUNT          908 a         1188 c         1316 g         1149 t
ORIGIN      
        1 gcctgagcct tctggagctt ccgaaggttc catcgggggt gagacggcag cgcctccgcc
       61 tgcgcctccg cctgcgcatg tgcctccgcc tccgcctcag tcgacaattg gagctggtcc
      121 ccagactggc atggcacctc cgtcagtcgg tgtgagtccc cagactggct ctgcacctca
      181 ggcagtcggt gtcagtaccc aggttgcggc ttgcgccatt gctatgccgg agcctatacc
      241 ttgcgcggtt ccgccagtcg agatcaaggc cagcactcgg ggcgatgaag ttgagctact
      301 ggagcgcacc cgcttaagcg gagatcagga tctgcatttg accggcagcc tgactgcact
      361 tcagttggga aatatggaag ctattatccg tgccaatcga gacaaaagtg taaagatagc
      421 atggtgcgcg gcgccccgtc tcgaaggcgg caaggtggta aaacgaccgc tcaagccggc
      481 tgtttatgag ggtgtcagtc gactttatgt agtcgtcgat ggttcctcgg ttatgtctaa
      541 atatttgaag tcagtggctg ccggtctgaa gtctttaccg tcttccataa attccaccat
      601 ttatctggtg ggtgatcaga gtttgcgttt tggtaactct ggtttcaagg gcgacgaaaa
      661 gccttacctg gaggccgtgc agaaggtcga aggctttgtg gccgtcggcg ggcaggacga
      721 ttccctggtg ctgtctgaag cactacacag ggccggtaag gagaaagaca gtgccgtctt
      781 atggatacat ggagcccagc ctctcagtcc cggcagcttt actcccattc agcggatgct
      841 agtaggcagt ccccttcgtc ccgtgctctt tgatttgcct gtcagttccg gacccaatga
      901 aatcctcaat ggtgtagatg ccgataccag aatcttgcgg gtgaatagaa gcggcgacct
      961 ggctgatgat ttgaagaatt tatttcatag cattgatccc agtttcgccg cctccaggga
     1021 gcaactggcc gcgcagcagt tacagtacgt cgactccagc ggactgacgg aagcgggcgc
     1081 gagaaaggtc agcccctggc tggtaaatat ttttgctgcc gctgaaatca agcgaaaaat
     1141 gtccggctgc cctactgcaa gaatttccga gagccccgcg cttctagctg acctgtgtca
     1201 gatagtcagc cccgtctctt ccgccattgt ggtctctcct gatagtcatg cctatgtggc
     1261 caagactgaa accgaagatc gctttttcca gtctgtgggt gaatttgtca gtcggatatt
     1321 tctgggatcg atcaatgcag tcggacctct ccgagaggtc gcgcctcgca agtattatta
     1381 cgaactgccg gcctcgtcgc ctgcgcctcc gcctccggtt tttcaagggc aaaatcctat
     1441 cgatagtgcc ttcaatggtg tcacccggca acttaatagt ctctccagcg ctgccggggc
     1501 tccgactggt tcctatggtg ccgccatcca gagcaaggcg gccaaacagt ctatgtctgc
     1561 cggtaaattt gatcaggccc cagcccggag tgatgctttc ggtggcggtg gtgatgcctt
     1621 cggtggcggt tccgatgctt ttagtggagg cggcggcgca tacgacggcg ctaaaggcaa
     1681 cgctgccggt gttagcgggg cgcccattgg tggcgatgcc cgcaggatga atgaacagac
     1741 ttacggtgca agcgatggac ccgccgctgg tgagccgctt ggcgatgcct acggttcctc
     1801 tattcccgtg gataaaaccc gggctctgga agagcttccc gcttccgttc cggctcaggc
     1861 gccgcctccc ccttcgccgc agggtttcac aaccgccaaa gacgaacttt ccatggctgc
     1921 accggtagcg gagaagaagt cagttattga tgccgagcgg gacagtatgg aaggagctga
     1981 tctttcccgg ctgaaggaag cgcaagcttc cggcaagggc cagagatttg atttccaggc
     2041 ttcgcgatac cgtcttgaga gcggtccggt ttttcagggt gccaccaacg gcaccattct
     2101 gccccaattt cagaacttcg gccaggacga cgccggaaag aatgcgggac gtgtaggcgc
     2161 cccggttcat tcccgcaacc gacagtacaa tcgtcttggc gccgaagaat acaatgaaga
     2221 ggcctcgctg caaaaaaccg ccaatctctt cacgttgatt gtagttgtgg ccggtcttgt
     2281 gatctggttt ctcttctacc gggggcgaaa caaagaagag gacgtctagg ctttagtttc
     2341 tcgtgtaatg atggcgctgt gaatgccatt taaatgagta agaaaaactg tcagttcggt
     2401 ttgaggttcg gcgtcatgct ttgccttttt gtgcgcgttt ttgagagcca gggctttgag
     2461 cagttcttcc ggagcaatgg gggagccgcg ctttttcacg accacgctgg tgcaagccag
     2521 ggcttgaagc tctgccttta acttcttgag gctgaatggc attgccgcca ggacttcgaa
     2581 ggagcgcatc aggggcgctt ttgccggtgt ggctccggtc aaataagcaa tatcctggtc
     2641 gattttgagt gcattgaaac gagcgcccac caactctaca agcccggcgc gaataatggc
     2701 accgtcaggt tcatgcaagt agcgagccag tggtgccact gccagtggtt ccatgccgtc
     2761 ggcatcgctg agggtgatta tttgtttgtc ttgatcgctc ggtgcggaat ttgaatcaac
     2821 ctcagctctg tcgttttcgt ccggtgctct ttcatccttg taccgtgttg gagctttctc
     2881 cttttgggac gtgagcaagg tggctctatg tctggcgact ttgccactgg tttccccgcc
     2941 gacctctttc aatgtgccga accataggag ggcttctttt acttcaccgt cctgggaaat
     3001 gatttcgatt tcgcaatcta ggtctttcag tttttcgtaa tcgatgccgg gagacagctt
     3061 aacagccagc ccctttaggt gaggaagcca gctatggatg gtactgaggg gaggcaggta
     3121 gtgctccgga ttacttatgc gccggccgtc cttggttctt cgtcctggat cgaaaaaggc
     3181 accctggtag gaactgatct tgcattcttt gacgtcttca tgtaagcccg caaagcgttc
     3241 ggcgtttcca taaacggcgg cattggcagc ggcaaagatt aacctggcct ggtcgagatc
     3301 gacccctgtg acggagaagt ggcggctcaa ggcagtggtg tcgccgccga taccgcagca
     3361 cagatcggca atggcagtgt tcgcttcaaa aactcgggcg aagcgccggc tgcggtaggc
     3421 ggcaatttct tcgccgctgg cttgctcaag ggcttccctg gtgaaaaaca tttgggcggc
     3481 gcggctgaat tttttggttt cggcagccct ctgcctggct tttgctactt ccaaaatagc
     3541 cgacgattgc gcttgattga gcctggcatg cttgcgcaac cattcgattg cgctcaattc
     3601 ctggggggct gctatggatg ggtagcgggc ggtcagctct ttcaggcagt cttcaccgga
     3661 tttagaaaga acaaagcgag cggtttcgag atccatttac ccttgcattc cttgtcggcg
     3721 atccttttgg cgcctacagg tgagaatcta tcttacttgg tgacgatgtt cagtttcagg
     3781 tcattgccgt tccatgcgct cttaaattcc ggtgtttgca cgcggccgcg gtcccgcaat
     3841 acttctgcct ggacgttatc tttaagttgc aggaagattt tgctaagagg cgtggtgccg
     3901 ccgtcggcag ccagaatatc aatcaactgt cttgtgaaga cgccgttcgg gtactgtttt
     3961 gattcccagg agacctgatc gggtgcgctg gagcagatga ccatctgccc ggtgccctgg
     4021 gccagtgcct gggagtcgaa gttgtcggcc ctgtagagcc ctttggctcc ttctcgagcg
     4081 gcaccgctat ggcaagcatc aaagaccagt aatactcttt ccgtcttcac tctctcttta
     4141 atcatgggca atagcttggt catggaaaga ccggtggcaa acagatgatt gggtttggtg
     4201 ttgtagacca ccaggtagtt gtcgcctttc acatccattt cggcagggct gccgtgaccg
     4261 gagaaataaa tgacaaccag gtcgtcaggc ttaactacgc ggggcagcca ggagtcgccg
     4321 atgagatcta cgatgttttc aagggaggct tctttgtcga tgagcaaacg ggtgttttct
     4381 tgtttgaagt taccggggcc ggtcagatag ctacaaaaat ctctggcatc tttggctgaa
     4441 tatttgagat tgagagaggg gtcttcaaaa ttgctgatgc cgatgacaag agcgtatttt
     4501 tgtccgatgc tgtttgcgac ctgtgggttg aatgtggcgc cggtgccagg ttttgtgcct
     4561 t
//
LOCUS       DAOQNZ010000117         4515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8376_length_4515_cov_4.471300, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000117 DAOQNZ010000000
VERSION     DAOQNZ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8376_length_4515_cov_4.471300"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PKW73_03175"
     CDS             complement(<1..1109)
                     /locus_tag="PKW73_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HNA72310.1"
                     /translation="MAGSSEESSAKKAKPLARSFACPACGGTVTIRYPGAALSCVCEF
                     CQTVIDATDSNYRIIEKYFSKTRHFEPFVPLGTRGKLKGKDWEVVGYMVRADEVSGYF
                     WDEYLLFNPYYGYRWLTCNGDHWSFVTTIKQKPQSRTHSRSTSWYKVKMELEGRDFKL
                     FYRGRAKVLFVLGEFYWKVKADSLVNMEDYISPPYMLSSESDGKEIVWSISEYLEVDD
                     VAAAFKPAKKLKRPSKVAPSQPSGAAVSWQYMSKLWLASFVILTVLQVWQLGYNNSRQ
                     VLDSSPVYTANAKNAPTYTTAVFNIDRDKTGALLEFSTSVDNSWLYVSGEMVNDDTGE
                     TFPFAQTIEYYHGYDGGESWSEGGTSNNVFLSAVP"
     gene            complement(1099..1725)
                     /locus_tag="PKW73_03180"
     CDS             complement(1099..1725)
                     /locus_tag="PKW73_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HNA72311.1"
                     /translation="MIKLSCPSCGAEVEFRSKASVFSVCSFCHSTLVRHDINLEVLGR
                     MSGLQDDLTPLQIGTKGVFDGQPFELIGRLKVGYEDGTWNEWYALSGDKVGWLAEAQG
                     FYAFCYPVTGIQAPDINRMVPRSPVYIEQVGQMQVEDARNVHCLFSEGELPFNAGLGR
                     KSFSVDLTGSNLAMATVEYAPDETRIFAGRYQDFDDFQFQNLREIDGW"
     gene            complement(1725..2720)
                     /locus_tag="PKW73_03185"
     CDS             complement(1725..2720)
                     /locus_tag="PKW73_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNA72312.1"
                     /translation="MFDIFKKQFIDVIEWTESGNGVLSYRFPTMDKEIQTGAQLTVRE
                     SQMALFVNEGNIGDVFSPGRYELNTKNLPILTTLLNWDKAFKSPFKSEVYFFSTREQL
                     DQRWGTQQPITLRDKEFGVIRLRAFGTFSYRIEEPTTFYQKVSGTKEVYTTTELDGQL
                     RSLIITNIAATLGEGKTPFLDMAANQLKLSETLKNSLDHAFWAYGLKLTSFLVQSVTL
                     PEELQQYLDKQSSMSLVGDLKKYAQFQAADAIAAAAENPGGVGMLGVGLNASALMGQA
                     FNQGMNEGGAAPAAAAEKQEDPFAVLEKLQTLHEKGILSKEEFESKKAEVLKRIS"
     gene            3048..3404
                     /locus_tag="PKW73_03190"
     CDS             3048..3404
                     /locus_tag="PKW73_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72313.1"
                     /translation="MKIDRVRRAILSLVLTVVSCFALFVLSHPLQESGVKRPLTSPDS
                     WVSTSMVRDYLSPISQFKLETHTSRFHSSGGLADLCFFSSTAGLYLRRFCESTHCLSN
                     APLQNKLWLVYCSLII"
     gene            3524..>4515
                     /locus_tag="PKW73_03195"
     CDS             3524..>4515
                     /locus_tag="PKW73_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA72314.1"
                     /translation="MHNLSLLQDLFVIWISALIAGQLFLRLRLPPIAGYMMAGIAVGP
                     NGLKLITDQEQIHVLAEFGVAMLLFALGVDLSLKQLKASAGKLILAGTSQLFLTVLAT
                     ALTAMALHLADSPGQAFLFGCVCAISSSVVISKILFDRGESDSVHGRILLSMAILQDL
                     SLVLIIPFMPILAAHGNTSWGSILISAMKAIGFTALIFFGAMRALPSILAGVARSNSR
                     ELFLLTLLGLCLGIALLSHQVGLSIALGAFLAGIMISESLYAHQALHDVQPLKDLFSV
                     VFFVSVGMLLDPAFIFSHCLEVVLFVILLISGKTVLAAISALFATANVRSALMVG"
BASE COUNT         1035 a         1245 c         1116 g         1119 t
ORIGIN      
        1 tggggtaccg ccgatagaaa gacattatta ctagtgccgc cctcactcca ggactcacca
       61 ccgtcatagc catggtagta ctcgatggtc tgtgcaaaag gaaaggtctc accggtgtca
      121 tcgttgacca tctcaccgga aacatagagc cagctattgt ctacggatgt gctgaactcg
      181 agtaaagcgc cggttttatc ccggtcgata ttgaagaccg ctgttgtata tgtgggcgca
      241 ttcttggcat ttgccgtata aaccggcgac gagtcaagca cctgcctact attgttgtag
      301 cctaactgcc agacttgcag caccgtcaaa atcacgaaag aggcaagcca gagcttggac
      361 atgtactgcc aactgacggc agcaccggac ggctgactgg gcgccacctt ggagggccgc
      421 ttcagctttt tggccggctt aaaagcagcc gctacgtcgt ccacctcgag atactcggaa
      481 atgctccaga ctatctcttt gccgtcggac tcggaagaga gcatataggg cggagagata
      541 taatcttcca tgtttacgag agagtcggct tttactttcc agtaaaattc gccgagcaca
      601 aagagcacct tagcccgtcc tcgataaaag agcttgaagt cccgcccttc cagctccatt
      661 ttcaccttgt accaggaagt ggaccggctg tgggtgcgcg actggggctt ctgcttgatg
      721 gtggtgacaa aactccagtg gtcaccattg caggtcagcc agcggtaacc gtaataaggg
      781 ttgaagagca aatattcgtc ccagaaatag ccggaaactt catcggctcg taccatgtag
      841 ccaactactt cccagtcttt tcctttcagc ttgcccctgg tgccgagggg tacaaagggc
      901 tcaaaatgcc tggtcttgct gaaatacttt tcgatgatcc gataatttga gtcggtagca
      961 tcaattacgg tctggcaaaa ttcgcagacg caagaaagtg ccgcaccggg atagcgaatg
     1021 gtgacggtac cgccgcaagc gggacaggcg aaactcctgg ccagaggctt cgccttctta
     1081 gcagaacttt cttctgaact accagccatc tatttccctc aggttctgaa actgaaagtc
     1141 gtcaaaatct tgataccgcc cggcaaaaat gcgggtctca tcgggggcat actccactgt
     1201 tgccatggct aaattgctgc cggtcaaatc aacgctgaac gactttctac caagccccgc
     1261 attaaagggc agttcaccct cgctgaagag acaatgcaca ttgcgggcat cttccacttg
     1321 catctgcccc acctgctcga tatagaccgg agagcggggc accatgcgat tgatgtcggg
     1381 tgcctgaatg ccggtgaccg gatagcaaaa agcataaaag ccctgtgctt ccgccagcca
     1441 gcctaccttg tcgccgctca aggcatacca ttcattccag gtaccgtctt catagcccac
     1501 cttgaggcga ccgatcagtt cgaaaggttg gccatcgaac actcccttgg tgccaatttg
     1561 caagggcgtc agatcgtctt gcagtccgga catgcgtccg agcacttcca ggttgatatc
     1621 atggcgaacc agagtggagt ggcaaaaact gcacacggaa aagaccgatg cctttgagcg
     1681 aaactccacc tcggcgccgc aagatgggca actgagcttg atcatcagga aattcttttc
     1741 aagacttccg ctttcttgct ctcaaactct tccttggaaa gaattccttt ctcatggaga
     1801 gtctgcagct tttccagcac ggcaaaggga tcttcttgct tctcggcagc ggcagccgga
     1861 gcggcgccgc cttcattcat gccctgattg aaagcctgac ccatcaaggc agaagcgttc
     1921 aatcccacac ccaacatgcc taccccaccg ggattttcag cggccgcagc aatggcgtcg
     1981 gcagcctgga actgggcata ttttttcaaa tctccaacca ggctcatgga agattgctta
     2041 tcaagatact gctgcaattc ttccggcaaa gtgacgctct gtaccaggaa gctggtcagc
     2101 ttcaaaccgt aagcccagaa agcatgatca agagagttct tgagggtttc ggagagcttc
     2161 agctgattgg cagccatgtc gaggaatgga gtcttgcctt cacccagggt ggcggcgatg
     2221 ttggtgatga tcagagaacg aagctgtcca tcaagttcgg tggtggtata gacctctttg
     2281 gtgccggaca ctttctggta gaaagtagtc ggctcttcta tgcgataaga gaaagtaccg
     2341 aaggcacgca ggcgaattac gccgaattct ttatcgcgca aggtaatggg ttgctgggtg
     2401 ccccagcgct gatcgagctg ttctctggtg gagaagaaat acacttccga cttaaaggga
     2461 gatttaaagg ctttgtccca gttgagaagc gtagtcagga tcggcaggtt cttggtgttc
     2521 agctcgtagc gaccgggact gaagacatcc ccaatattgc cctcattcac gaagagagcc
     2581 atctgggatt cccgcaccgt aagctgggcg ccggtctgta tttccttgtc catggtggga
     2641 aagcgataag acagcacccc gtttccactc tcggtccact cgatgacatc gataaactgc
     2701 ttcttgaaaa tatcaaacat cgaaaaatct cccaatccgg tcgaattttt gtactaaata
     2761 catggcttga ggctgcctga aggtgcccga gggtacttcc aaatttcttg ctaaggatag
     2821 caagtttcaa gggcttctat atgagtgttg cccggggaaa gaaccggcac ggtcgactct
     2881 cttaaatcag ttctgtttct tccttcattc aagccttgcc aagcgcttgt atgcctaagc
     2941 tacaccccgg tagaccccat atgccaaagg tcatgatttt tcttaactat ttcccggctc
     3001 cggtctaatc ttcacccgga tgttaaaatc gagaaaaggg gggagatatg aaaatcgaca
     3061 gagtcagaag agcaattctt tcactggttc tcacagtcgt atcttgcttc gctctctttg
     3121 tgctcagtca ccccctgcag gaaagcggcg tcaagcgtcc cctgaccagt cctgacagct
     3181 gggtaagcac ctccatggtg cgcgactact taagtccgat tagccagttt aaactggaga
     3241 cccacaccag tcgtttccat tcatcaggcg gcttagccga cctgtgcttt ttcagcagca
     3301 ctgccgggct ttacctgaga aggttttgcg agagcaccca ttgcctcagc aatgcccctc
     3361 tgcaaaacaa gctctggctc gtttactgca gcttgataat ttaagaagca gcttcggctc
     3421 gtcgtcttta ccgcagggct gccaaattca cgaaatatcc gtctcagtga ataaattcgc
     3481 actggcataa tttggcaccc ctctaccgaa gagagtggta caaatgcata atctttcctt
     3541 gctccaggac cttttcgtca tctggatcag tgctctcata gccgggcaac tgtttctgcg
     3601 actgcgtcta ccgcccattg ccggctacat gatggcgggc atagcggtgg gccccaatgg
     3661 tctcaaatta atcaccgacc aggagcagat tcatgtgctg gctgagttcg gcgtggccat
     3721 gcttctcttt gctctggggg tggacttatc actgaagcaa ctaaaggcct cagctgggaa
     3781 actgatacta gccggaacca gtcagctttt tctgacggtc ctcgccaccg ctttgacggc
     3841 aatggccctg cacctggccg acagccccgg gcaggccttt ctcttcggct gcgtttgcgc
     3901 catctcctcc agtgtggtca tctccaagat tctcttcgac cggggcgaaa gcgactcagt
     3961 acacggccga atccttcttt ctatggctat cttgcaagac ctctccctgg ttctaatcat
     4021 cccgttcatg ccaattctcg ccgctcacgg caacacaagc tggggcagta tattaatctc
     4081 cgccatgaaa gcaataggct tcacggctct aatttttttc ggagccatgc gcgccctgcc
     4141 ctccattctg gcaggcgtag cccgttccaa ttctcgggag cttttcctgc tcaccttgct
     4201 gggactttgc ctgggtatcg ccctgctctc ccatcaagtc ggcctgtcca tagccctggg
     4261 agcctttcta gcgggcatca tgatttcgga gtctctttat gcgcaccagg ccctgcacga
     4321 tgtacagccg ctgaaagatc tcttctccgt tgtcttcttt gtatcggtcg gaatgttgct
     4381 tgatccagca tttatcttca gtcattgcct ggaagtagtg cttttcgtaa tccttctcat
     4441 ttccgggaaa accgtgctgg cagcaatttc cgccctcttt gccacggcca atgttcgaag
     4501 cgcactgatg gtagg
//
LOCUS       DAOQNZ010000118         4513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8385_length_4513_cov_3.190220, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000118 DAOQNZ010000000
VERSION     DAOQNZ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8385_length_4513_cov_3.190220"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1012
                     /locus_tag="PKW73_03200"
     CDS             284..1012
                     /locus_tag="PKW73_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72315.1"
                     /translation="MPPGASTGQSQPVATTASDKAARGESQWLFLQEVADLSGLSEKT
                     VRRHIKKGTLKAKKGKAANARIQVLITPEILASISKESPTEELEEDAGFENIDFEEVS
                     AEEVTEETASDGVTDFSPNPAEKMQADMEMFKGIINEMMAPLVKRVEEQARTLHEQER
                     VIQDQKTQLRLLPDIQAREESERKAREMAELEKQALVKQIEALKTAQEELKATAEKAA
                     ELEKQLTIAKRPWWQKLMGSPQES"
     gene            2116..2973
                     /locus_tag="PKW73_03205"
     CDS             2116..2973
                     /locus_tag="PKW73_03205"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNA72316.1"
                     /translation="MPNQKYATSAAFKVALETRLKKAADERGRPYHVLRKLVSFDRLL
                     ARIFMDKDETFALKGGYSLELRLGRSRTTLDVDLVVREKSLKQAGSIEEAIRDLLQER
                     LEQDLNDYFSFRLGKPTKLTAATIGGFRIPVKSLLGGTQFEDFHLDVAVGETDPLPLE
                     ELWGESWLEFANIDQAKPLAIPASLQFSEKLHAYTFPWQERENTREKDLIDMAVLIEQ
                     GLDKIDTSKRVSDVFRYRKTHELPEELPEPPSSWGKVFETMAVEIGFEGTIETAFNMV
                     KEFYRSLKL"
     gene            complement(2991..3611)
                     /locus_tag="PKW73_03210"
     CDS             complement(2991..3611)
                     /locus_tag="PKW73_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA72317.1"
                     /translation="MNNNPAVVFDACVLYPAPLRDLLLELAGYAQDRGWFRAKWTEEI
                     HSEWIRNLLANREDLTNAQLDRTKALMNKHIDDCLVTGYEHRISSLTLPDQNDRHVLA
                     AAIECGASIIVTANVSDFPAEIVLPSGVQAMTADDFIAGLISTFESEGESALEASARA
                     IKERLKNPAMTWSQYFDCLLTMSGNELTKTVGLLRDIVPASEVAAK"
     gene            complement(3618..4076)
                     /locus_tag="PKW73_03215"
     CDS             complement(3618..4076)
                     /locus_tag="PKW73_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA72318.1"
                     /translation="MHSGAERRSVVPSPAEVEQAQTSSRKIAELTAKASPQQRYALTD
                     CKGTEIELGESACRVLLEALNAIAGGHSVMLVPIETELTTQQAAEMLNVSRPFLIGLL
                     ESGEIPHRRVGRYRRIKRQDILEYQARRADRREEIMEELASQAQELNMGY"
BASE COUNT         1348 a         1167 c         1059 g          939 t
ORIGIN      
        1 cccgcctgtc cagacagaac catgatgcta ccagcccaac tgctactctt gtccagacaa
       61 gccacaagcc catcactacc ccacgctttc agactggaca ccagccagcg cacaagaact
      121 ccagccacca ccaccagttc caaagcgccg ataaatttat atccacaaga catatccaca
      181 cccgcaaaca caaaatatgt ggcgcttttg aagaaaacga atataattgt ctagtcaatc
      241 gatttgtcta gacaactcaa gcaactagaa ctggagtacg cagatgccac caggagcatc
      301 cacaggacaa agccaaccag tagccaccac cgctagtgat aaggcggcaa gaggcgaatc
      361 tcagtggctc tttctacagg aagtggcgga tctttccggt ctttccgaga agaccgttcg
      421 cagacatatt aagaagggaa ccctgaaagc caaaaagggc aaggccgcca acgcaagaat
      481 ccaggttctg attacaccgg agatcttggc cagcatatct aaagaatccc cgactgagga
      541 gctggaggaa gatgccggct tcgaaaacat agattttgaa gaagtctcag ctgaagaagt
      601 taccgaagag acagcaagcg atggagttac agacttctcg cctaacccgg ccgaaaagat
      661 gcaagcggac atggaaatgt tcaaaggcat tatcaatgaa atgatggctc cgctggttaa
      721 acgggtagaa gagcaagcca gaacgcttca tgagcaggag cgcgttatcc aggatcagaa
      781 aacgcagctg cgccttctgc cggatattca agccagagaa gaatccgaaa ggaaagccag
      841 agaaatggct gagctggaaa agcaggcttt agtgaaacaa attgaggccc tgaagaccgc
      901 ccaagaggaa ctcaaggcga cagccgagaa agcagccgaa ctggagaagc agctcaccat
      961 tgccaaacgc ccctggtggc agaaacttat gggaagcccc caggagtctt gagccctcga
     1021 tagagacggg cggactccag cgtgcgtggc actaccctgg tgaacggatg agcctttgcc
     1081 ggcaccatag accggggaag aatcctcgat gtggccaatc cataaatcgc ccaccgcgta
     1141 gacaacgaag cgtaattggc ttcatggact gtcgaaattg agatacgcag acagtgggca
     1201 ttcttgcgat agccggcatt actcgattct cacagcggcg gcttcgcgca ttccatgtgg
     1261 acgagagagg ctttctccta gaatgtaggt gaccttcgga agtctgttga atcgttacta
     1321 gaagcccaat tcagcagacg gcctgaacct agtccctaag accgcttcac agcaattgtt
     1381 gccagccggc tcttaaaaaa ggggatgttc aatgaggcag ataaaggggg ggctctgagt
     1441 aacgtgcaaa aacctcctac ttccgatagt acataaatac cccactaatt gtggtattta
     1501 tgtactaccg ctccccatag accaagaaca aaatgagaaa cccagtcgaa aaaagaatca
     1561 agcttcttga aatgagctct ccccagtttg gactctttac ggccaaacag gcagaggcat
     1621 gcggctacag cagccaccac ttcaatcacc acgtaaatag cggcgagtgg gtaaaggaag
     1681 acaggggact tttcaggcta gccacatacc cggcctcgcc agaacaggtt tatatgcgct
     1741 ggcagctctg gacaaggaac cggcaggata gaccgcaagg ttgcctttct cacgaaactg
     1801 cactattcat ctacaaaatt agcgacatcc tgccaaacaa aatacatatg acagtgccaa
     1861 gagacttccg gcgaaacata cagatacctg gcattctcgt gcttcaccat agaaatctac
     1921 cggcagatag cgtgcaaatt gaaagtgggc tcacgttgac tacaccagca agaacactgg
     1981 aagaccttct tgataccggc acagttgaca agtcaataat ccaagaaagc ctccacaaag
     2041 caatcgagat aggtctgatt acgaaaaggc agctagatca aagttcaagc ctttcgaaac
     2101 taagagatta gaagagtgcc caatcaaaaa tatgcaacca gtgcagcctt caaggtcgct
     2161 cttgaaacaa gactaaagaa agccgctgat gagaggggcc ggccttatca cgtcttgcga
     2221 aagttagtct cgtttgaccg actcctagca agaattttca tggataaaga tgagacgttt
     2281 gctctgaagg gcggatacag cctggagctg agactaggaa gatctcgaac aacgctggac
     2341 gtcgacctgg tcgtcagaga gaaaagccta aaacaagcag ggagtataga ggaggctatt
     2401 cgagaccttc ttcaagaacg cctggagcag gatctcaatg actacttctc attccgactc
     2461 gggaaaccta caaagctaac agcagcaacg ataggaggct ttagaatccc ggtgaaatcc
     2521 ctccttggag gaactcagtt cgaagatttc catctggacg ttgcagtggg agagaccgat
     2581 ccgctccctt tagaagaact ctggggtgaa tcctggctgg agtttgcgaa tatcgaccag
     2641 gcaaaaccac ttgccatacc ggcgagcctt caattttcag agaaactaca tgcatacact
     2701 ttcccctggc aggaacgaga aaacacgaga gagaaggatc tgatagacat ggctgtcctt
     2761 atcgaacagg gtttagacaa aatcgacact agcaaaagag taagcgatgt cttccgctac
     2821 agaaaaactc acgaactgcc cgaagaactt ccagaaccac cttcgtcttg gggaaaggta
     2881 ttcgaaacga tggctgtcga aattggcttt gaaggaacca tagagacagc tttcaacatg
     2941 gtcaaggaat tctaccgaag cctgaaactt tgatgagaca agacaacttg ctattttgca
     3001 gcaacctcgc tcgccggaac gatatctcgc agcagcccaa cggtttttgt aagctcgttt
     3061 ccgctcatag ttaagagaca atcgaaatat tgactccagg tcatggccgg attcttgagc
     3121 ctttctttga tggctcttgc tgaagcctcc agggcagact caccctcaga ttcaaaagta
     3181 ctgatcaacc cagcaatgaa atcatcggcg gtcatagctt gtactccgct aggcaataca
     3241 atttcagcag gaaagtcaga gacgttggcc gtgacgatga tactggcacc acactcaatt
     3301 gcggcagcca aaacatgcct gtcattttgg tcaggaagag tcaggcttga aattcggtgt
     3361 tcgtatccgg taactagaca gtcgtcaata tgcttgttca tgagggcctt agtacggtcc
     3421 aattgagcat tagtcagatc ttctcggttc gccaggagat tgcgtatcca ctcactgtga
     3481 atttcctcgg tccatttcgc ccggaaccag cctctatctt gtgcgtaacc agccagctct
     3541 agcagtaaat ctcggagcgg ggcagggtac aggacacaag cgtcaaaaac aacggccgga
     3601 ttgttattca taggtggtca ataacccatg ttcaattcct gagcctgtga ggcaagctcc
     3661 tccataattt cctctcgacg atcagctctg cgagcctggt actccaaaat atcttgacgc
     3721 ttgattctgc ggtaacgacc gacacggcgg tgcggaatct caccagattc tagaagacca
     3781 atcagaaacg gtcgggaaac gttaagcatc tcggccgctt gctgtgtcgt taactcggtt
     3841 tcaataggga caagcataac tgaatgcccg ccagcgatag cattcaaagc ctcaagaagg
     3901 acccgacatg cggactcacc tagctcaatt tcggtgccct tgcaatccgt aagcgcatat
     3961 cgttgttgtg gcgatgcctt agcagttagc tcggcaattt tccgcgatga ggtttgagcc
     4021 tgctcaactt ctgcaggact tggcacaacc gatcgtctct ctgctccact gtgcataagc
     4081 agacctccaa gcgaaccaca aaccatttgg tggcaatcat accacatagt cgaaatactc
     4141 gcaacagtcg aaacggtcga aatcaaccaa ggctcagcaa gagcaaggca aagtaaacaa
     4201 aagggatatt ttgttcacta cgcaatcccc accccacgac cctcccaggt cttgcactca
     4261 aaactccctc ggttataata aacaatactg attcatacat aagtattgtt cacatgccag
     4321 gcaaagctaa agtcctaagt tccaaagaga tccaaaacgt tctcaaggtg ctgaaatccc
     4381 cccgagataa actcctcttc gctgccggtc tctacaccgg tctccgcata tcggaaatca
     4441 tcggaataaa acaagaacat gtctacaccg ccagcggtgg catcaggaat atcctaaaaa
     4501 tcacccgcct caa
//
LOCUS       DAOQNZ010000119         4498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8428_length_4498_cov_3.194013, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000119 DAOQNZ010000000
VERSION     DAOQNZ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8428_length_4498_cov_3.194013"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PKW73_03220"
     CDS             <1..919
                     /locus_tag="PKW73_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72319.1"
                     /translation="NFRLRFDDSNSGPEGLAALRDAYYSRHDSRLNLVAYWPEKGDGK
                     VAVAHLLPSVAIVDDSTSNAPPKPSAVDPYIEESMSAPSVTGNIAPDSPGLNCLIGAR
                     RGYSVPWEKMEDINLTLTPNNVDTATLAKIMNLPGASGEEVLKTRLARLYKFDETLPV
                     QAIVSQAKAIEQSRWKAALGLESEASSQQIDTAFGRYMMDQSHSTEVRPFVYVDRAAD
                     QLAAKVLGVSSPESLSHAQVDALLAERLASNQAAMMNLPAGTTWAESQRIAMARLNDL
                     PDSASEGEIMLAQFLHGNDYPSVNPDCGS"
     gene            complement(968..1927)
                     /locus_tag="PKW73_03225"
     CDS             complement(968..1927)
                     /locus_tag="PKW73_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNA72320.1"
                     /translation="MSQETRPESPRQERPTETTPPASTNPSTRMMEEVLSQRPNPPVT
                     DLNGRECRNQPENGQIPVLRAGEKTSGCNFQSFDRTPEGSTMPPFNTGQLYEQAQRQT
                     ARIKVEEAGGGGGHGTGVAVARNENTCFIATANHVSGERGNQNIVSRGAEFENGKTYP
                     ASIEMRIPGKDMAILSVRTGADTNQVCHPAQIAANPFDRSDRSGGGQTMTAGFPEGTN
                     TLHASPGRISRVGTYEALTGGAHPSPMAGERVNRPLIETRAQIHQGNSGGPTYNNRGE
                     VIGLSSGVHPDERKARSSAQITPISRAEIGRILQRIPGAAPLP"
     gene            complement(2228..3241)
                     /locus_tag="PKW73_03230"
     CDS             complement(2228..3241)
                     /locus_tag="PKW73_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNA72321.1"
                     /translation="MQNPRSISSERLKLKFNATKHVLLLDLDNTLTDTRRWFANFILN
                     STEELAHALKIEPELLNRYYGDVALATTLHEYAFVVETIAAKVKAHSHLPPQKIMALA
                     ESFWEGFQKHHQNIEVYEGVHQTLAKLRSHFRSLDIIILTDSPDWVAIDRLALTGLLP
                     LVHGLVAIRTEMPELRHSRYRDCLQKSKDRILAIKHKHDTRHLKLKLAISKACAKPSC
                     AGIELIAKALDGALGELMICGDKESKEGMAAQNWRQYQRLTGEPTKDIHFIKADYGNY
                     DLTHHLYQELEKQIHSLKAPKVNEQMVSAGPPVFRALERCDQLTEVIEEIISGKPSLI
                     EVA"
     gene            complement(3658..>4498)
                     /locus_tag="PKW73_03235"
     CDS             complement(3658..>4498)
                     /locus_tag="PKW73_03235"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNA72322.1"
                     /translation="PPKGAGVKKITAAAELLYKAGADAINVGDSPMARVRMSSLATCQ
                     LLSQRVGVDTIIHFTTRDRNLMGMQADLIGCQALGLKNILALTGDPPALGNYAHATAV
                     YDVDSIGLIKIIKQLNEGKDIAGNTIGAPLTLSIGCALNPTAQNRTLEMERIKRKIEA
                     GADFIMTQPIYRVSDLTDFLEEMGGSPIPVLVGIMPLHSSKHAEYLHNEVPGITIPKH
                     IREAMAKAGEDGARIGLELAEKLLEELRDTIEGTYLVPSFGRYDDMAALVTRLKSKVR
                     ALK"
BASE COUNT          892 a         1091 c         1282 g         1233 t
ORIGIN      
        1 gaattttaga ttgcgttttg acgatagtaa ttccggaccg gagggattgg cagctttgcg
       61 tgatgcttat tattcacgcc acgatagccg gctcaatctg gttgcttact ggccggagaa
      121 aggtgacggc aaggttgccg tcgctcatct tctcccgtcg gttgctatcg ttgatgattc
      181 cacatccaat gctcctccca agccatctgc ggtggacccc tatattgagg agagcatgag
      241 cgcaccatcg gtgaccggca atattgctcc ggactcacca gggctcaatt gcctgatcgg
      301 agctcgtcgc ggttacagtg tgccctggga gaagatggag gatattaacc tcactctcac
      361 tcccaataat gttgataccg ctacccttgc caaaatcatg aacttgcccg gtgccagcgg
      421 cgaagaggtt cttaagacgc ggttagctcg gctctacaaa tttgatgaaa ccttgcctgt
      481 ccaggctatt gtcagtcagg ctaaggctat agagcagtcg cggtggaagg cggccctcgg
      541 tttagagtcg gaagcctcgt cccagcagat tgatactgca tttggtcggt atatgatgga
      601 ccaaagtcac tcaactgaag taagaccttt tgtgtatgtc gatcgcgctg ctgatcaatt
      661 ggcggcgaag gtgcttggcg tgtcttcgcc agagtctctc tctcatgctc aggtcgatgc
      721 tctattggct gagcgactgg caagtaatca ggctgccatg atgaatttgc cggctggaac
      781 aacctgggca gagtcacagc gaatcgccat ggcacgcctc aatgatttgc ctgattccgc
      841 ttccgagggg gaaataatgt tagcgcagtt cttgcatggt aatgattatc cctcagtcaa
      901 tccagactgc ggcagttgaa tgtatgggtt tgcctgaaat ccgaaaagcg attgctgctc
      961 atttagatca aggcagcggt gctgctccgg gaatacgttg caggatgcgc ccgatctcgg
     1021 ctctggagat tggtgttatc tgtgctgaag atctggcttt gcgttcatcg gggtgcacac
     1081 cggatgacag accgattact tcgccgcgat tattgtaggt gggaccaccg gaattgccct
     1141 ggtgaatctg tgctctcgtt tctattaagg ggcgatttac tctttccccg gccatgggcg
     1201 aaggatgggc gccgccggtg agggcttcgt aggtgccgac ccggcttatg cgaccgggag
     1261 aggcatgcag ggtgtttgtc ccttcgggaa atccggctgt catggtctga ccgccgccgc
     1321 ttctatcgga tctatcaaat gggttggcgg caatctgggc cgggtggcag acctgattgg
     1381 tatcagcacc ggtgcgcact gatagaatgg ccatgtcttt gccgggtatc ctcatttcaa
     1441 tgctggccgg gtaggtcttg ccgttttcaa attcggcgcc gcgagaaacg atgttttgat
     1501 tgcctctttc acctgaaaca tgattggccg tggctatgaa gcaagtgttt tcgttcctgg
     1561 caacagccac gccggtgccg tgaccgccgc cgccgccagc ctcttccact ttgatgcgag
     1621 cggtttgtct ctgggcttgt tcatagagtt gtccggtgtt gaagggcggc atggtgctgc
     1681 cttccggtgt gcggtcaaaa gactgaaagt tgcagccgct ggttttttcg cctgctctca
     1741 gcaccggaat ctgtccgttt tccggttggt tgcggcattc tctgccgttc agatcggtga
     1801 cgggagggtt tggtctttga ctgagaacct cctccatcat tctggtgctt gggttggtgc
     1861 tggcgggtgg agtggtttca gtggggcgtt cctgccgggg actttccggt ctcgtttcct
     1921 ggctcatttc ggtaccctca agaaaattta cccacaggca agccaacgcc aagtaccctt
     1981 tcgaaggttg gcttttggtt cagtagagtg gaggtgggta gtatggtggg aggtaggatt
     2041 ttcaacaaaa ccgaggtggt tttgacctac ctcgttgtcg atttggcgcc agtatatcgg
     2101 ccgcaatgta aaagaatgag aaattccgct tctctgtact gaacggaggg cggcgaaaga
     2161 aggagggctg cgctgggcag cctctcccat tcaatcttga gaatctaaat cttctacgta
     2221 tttcgggcta ggccacttca attagggagg gctttccact gatgatctct tctatgactt
     2281 ccgtcaattg atcgcatctc tccagcgcgc gaaacaccgg tggacctgcc gacaccattt
     2341 gctcgttaac tttgggcgcc ttgagcgagt ggatttgttt ctcaagctct tgatagaggt
     2401 ggtgagtgag gtcgtagttg ccgtagtcag cctttataaa atgaatgtct ttggtcggtt
     2461 cgccggtcag tctttgatac tgtcgccagt tttgtgcggc catcccttcc ttgctttctt
     2521 tgtcgccgca gatcattagt tctcccaggg cgccgtcgag agcctttgcg attagctcta
     2581 ttccggcaca gcttggcttg gcgcaggctt tggagatggc taacttaagc ttgaggtgcc
     2641 ttgtgtcatg tttgtgcttt atggcaagga tcctatcctt ggatttctga aggcaatccc
     2701 tgtagcggct gtgtctcaat tccggcattt cggtgcgaat ggccacaagt ccatgcacga
     2761 gcggaagcaa gcccgttagt gccagtctat ctatggcaac ccaatccggg gagtctgtga
     2821 gaatgatgat gtccagactt ctgaagtgag aacgcaattt tgccagggtt tgatgaacgc
     2881 cctcatagac ttctatgttt tgatgatgtt tctggaaacc ttcccagaaa gactccgcca
     2941 gggccattat cttttgaggg ggcaaatggc tgtgggcctt gaccttcgcc gcgatggtct
     3001 cgactacgaa tgcgtattcg tggagagtcg ttgccagcgc cacgtcgccg taataacgat
     3061 tgaggagttc cggctcaatc ttgagagcgt gcgccaattc ttcagtggaa ttgagaatga
     3121 agtttgcaaa ccatcttctc gtgtctgtga gcgtattatc gaggtcaagc aaaagaacat
     3181 gtttggttgc gttgaacttt agtttcaggc gctccgacga aattgatctt ggattttgca
     3241 tggcgtatct ccttgagcgt tttaatatgt ttttttggtt ctccttttgc cctattaatc
     3301 gagcggtgac ttctgtttga ggacgcacac aagtagcagg cacaggcacc ggcaactacg
     3361 aaaggttatt ttcgcggtcg ggcttatggc tgcggacgtg agtcttgggc gcaatagttg
     3421 gtattgcgct catgtcgcca acttcaatta gagttgcttc attgttgtca ccggcgccgc
     3481 gggatgtcaa tgggaatatt tttttaggaa ctgcaaggca ttgtctgaag gcgctgttgg
     3541 agttttagac caattttgcc gcttcttccc tggttgcaga tatggaattg tcacaaacgg
     3601 tcgacgacga gcgaggcatc gtcgatggtg gcagagcaaa tgctaaatgc gaacgcccta
     3661 tttgagcgct ctcaccttcg atttaagcct tgtcaccagt gccgccatat cgtcgtagcg
     3721 gccgaaggaa ggcacaagat aagtaccttc gatggtgtca cgcagttctt caagtaattt
     3781 ctccgctagc tccagaccaa ttctggcacc gtcttcaccg gctttggcca tggcttcgcg
     3841 gatatgcttg ggaatagtga tgcccggtac ttcgttatgg aggtattcgg cgtgtttgga
     3901 gctatgcagg ggcatgatgc cgaccagcac tggaatgggg gatcccccca tctcttccag
     3961 gaaatcggtc aggtcgctca ctctatatat gggttgggtc attatgaaat cggcaccggc
     4021 ctcgattttg cgcttgatgc gctccatctc cagggtgcgg ttctgagcgg tggggttcag
     4081 ggcacagccg attgagagag tgagaggtgc gccgatggta ttgccggcga tatccttgcc
     4141 ttcgttcaac tgtttgatga ttttgatcag accaatggaa tctacatcgt aaacagccgt
     4201 agcatgggcg tagttcccga gagccggcgg gtcgcctgtt agggccagga tgttcttcag
     4261 tccaagggcc tggcagccga ttagatctgc ttgcataccc atcaagttgc ggtctcttgt
     4321 ggtgaagtga ataatggtat ccactcccac tctctggctc aaaagttggc aggtggccag
     4381 tgaagacatg cgtactctgg ccatggggct gtcgcccaca ttgatggcat cggcaccggc
     4441 tttgtacaaa agttcagcgg ctgccgtaat tttcttgacg ccggctccct tcggcggg
//
LOCUS       DAOQNZ010000120         4473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8499_length_4473_cov_3.123359, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000120 DAOQNZ010000000
VERSION     DAOQNZ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8499_length_4473_cov_3.123359"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PKW73_03240"
     CDS             <1..860
                     /locus_tag="PKW73_03240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA72323.1"
                     /translation="GFAAQIATLKKPVDIVVATPGRLLDCVERRYLDLSHVEILILDE
                     ADRLLDLGFMPQLRRIISKLPKERQTLMFSATLEGKVATIASEYMLDPVTVRVNTERL
                     DVDSIDQRFVPVKEYNKDDMLIDLIRGINEECLGDPEKGSVLIFTGTKRKASWVKDRL
                     RDGGIKAEEIHSDISQIKRESTLKRYRSGAFTVLVATDVAARGLDIPAISHVFNYDLP
                     ELPEDYIHRIGRTGRAGRAGVATTFVSDEQRHLLAAIENIIGIKREKPKLSPAKQAAV
                     KRFQSRFRR"
     gene            complement(885..2213)
                     /locus_tag="PKW73_03245"
     CDS             complement(885..2213)
                     /locus_tag="PKW73_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNA72324.1"
                     /translation="MKKSRRRFLQELALKPFESGQEPQPKGTEARPGTNARAHGYANA
                     RAHANAHTLVVVFLRGGADTLNMLVPYGDDDYYRFRPSLAIQPQAKAGKNGFLPLDSF
                     FGLHPRLAPLLPIYKEGRLGFLPAVGSDNLSGSHFDAQDQMEHGVAWRSTASGGWLGR
                     YLSLSGGHSLGPLAAVSLSPSLPESFRGAPAASALSGVEEISIKAEGAPHLTGVLQKL
                     YKACPEPELREAGLNTLALLARISELRKAELAGPGDAALFPVTSFGQGLFQIARLVKA
                     GVGLNVACLDLDGWDTHFVQGSAEGLQAGIIAELGQGLAAFDNCLGPLMKEVTVVVMT
                     EFGRRVYENSSLGTDHGRGFTLMAMGGGIRGGQVHGQWPGLQEDLDELRLANLAPTGL
                     AVKVDYREPLLEILQGVMGAGAYGERIFPGFRPGFRSGFQQKSGTGLVQM"
     gene            complement(2215..3699)
                     /locus_tag="PKW73_03250"
     CDS             complement(2215..3699)
                     /locus_tag="PKW73_03250"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNA72325.1"
                     /translation="MTVSRRDVLLTAAFAGLSLSGCTMLRNAVAENLGERVPDKLCRT
                     WGKDIDPDFHLLSRAGFGGYPGDLERLKKIGRKAWLEEQLNPEAIDDKACDLRSRRFE
                     SLEIDPGTAYEFKREALRSDMVRHSLLKAVYSRRQLLEVMVEFWSDHFNISLEKGDCI
                     YLKPTDDRKVVRAHALGKFSDLLLASARSPAMLVYLDGKDNKRRNLMEKPNENYAREL
                     LELHTMGVNGGYTQLDVLNVARALSGFRLHDRYGRGQAYFDKSCHDDGEKLVLGKRIA
                     PGGGEKDLRAVLDIVLAHPSTARYIAYKLTRHFVSDEPPPQLCALVAETFTDTGGDIR
                     AMLRTIFDAEQFYDPCLRGAKFKRPFRFVVSSLRSLAADTDASKELCQFLSRMGQAPF
                     QYPTPDGYPVEREPWLGSLLWRWNFALTLCLGGIEGASVKNSMARLEAALADGKADPA
                     LLFPYFTGRNPLPEELSAFQEFAQEAHRRELFGLILASPAFQMY"
     gene            complement(3699..4235)
                     /locus_tag="PKW73_03255"
     CDS             complement(3699..4235)
                     /locus_tag="PKW73_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72326.1"
                     /translation="MMLADTMAVFFVVVGLILTLPALWLTLLGLWPGVVEASCQASSR
                     KLWKSFLVGLPFLVLTVILVGVFSKLPEGIGGMLAIAAIAFALLFANIGLSGLVTLVG
                     RRLPSPRDQVRPWLPTLRGGLLMVLSFILPLLGWFLLLPAALVIGYGSFVRGLLALRR
                     QAPPDAVVNVEVPSQVAS"
BASE COUNT         1123 a         1362 c         1158 g          830 t
ORIGIN      
        1 ccggttttgc cgcccaaata gccactctca agaaacccgt cgacatagta gtggcgacgc
       61 cggggcgttt gctcgattgc gtcgagcggc gctacctgga tctttctcac gtggaaatac
      121 tgatcctcga cgaagcagac cgcttgcttg atctgggctt catgccgcaa ttgaggcgga
      181 tcatttccaa gttgccaaaa gaaagacaga ccctgatgtt ttccgccact ctggaaggca
      241 aggtggccac aatcgcctcc gagtacatgc tcgacccggt gactgtgcgc gtcaacaccg
      301 aaagactgga tgtagattcc atcgaccagc gcttcgtgcc ggtgaaggaa tacaacaaag
      361 acgacatgct catcgacctg attcgcggca tcaatgaaga atgcctgggt gacccggaaa
      421 aaggctcggt cttgatcttc accggcacca agcgtaaggc tagctgggtg aaagaccgcc
      481 tcagagacgg cggcatcaaa gcagaagaaa ttcactccga cataagccag atcaaaagag
      541 aaagcaccct caagcgttac cgcagcggcg ccttcaccgt gctggtggcc accgatgtag
      601 ccgccagggg tctggatatt ccagccatca gtcatgtctt caactatgac ctgccggaat
      661 tgcctgaaga ttacattcac cgcatcggcc gcacaggacg cgccggtcgg gccggcgtag
      721 ccaccacttt cgtctcggac gaacagcgcc acttgcttgc cgccatcgaa aacatcatcg
      781 gcataaagcg tgaaaagccc aaactgagcc cggccaaaca agcagccgtg aaacgcttcc
      841 agtcaagatt tagacgctaa attttggcgc taagcagtcg tcgatcacat ctgcaccaat
      901 ccggtgccac ttttctgttg aaagccggac ctaaaacctg gccgaaaccc tggaaatata
      961 cgttcgccat aagctcccgc acccatcacc ccttgcaaaa tctcaagcag cggctcacgg
     1021 taatcaactt tgacggccag cccggtgggg gctaaattgg ccaggcgcag ctcatcaaga
     1081 tcttcctgca agcccggcca ttgaccgtgc acctgaccgc ctctaatgcc gccgcccatg
     1141 gccatcaaag tgaaaccgcg accatgatcg gtgcccaggg aagaattctc atagacacgg
     1201 cgtccaaact ccgtcatcac caccacggtg acttctttca tgagcggtcc aagacaatta
     1261 tcgaaagcgg caaggccctg accaagctcg gcgataatgc cggcctgcaa accttcggcc
     1321 gagccctgca caaagtgggt atcccagccg tccagatcca ggcaagcaac attcagaccc
     1381 acccccgcct tcaccagccg ggcaatttga aaaagcccct gcccgaagct cgtaaccggg
     1441 aaaagtgcgg catcaccggg acccgccaac tccgcctttc ttagttcgga aatgcgcgca
     1501 agcagcgcca gggtattcag ccccgcctcc cgcagctccg gctccgggca ggctttatat
     1561 agtttctgca aaaccccggt caggtgcggt gccccttcgg ccttgatgga aatttcttcc
     1621 acgccggaaa gggcgctggc ggccggtgcc cccctgaatg attccggcag ggaaggactg
     1681 agggagacgg cagcgagcgg tccgaggctg tgccctccgc tcagcgaaag gtagcgcccc
     1741 agccagccac cactagcggt actacgccag gcgacaccgt gctccatctg atcctgggca
     1801 tcaaagtgag aaccagacaa attatccgaa cccaccgcgg gcaaaaaccc cagccgtcct
     1861 tccttgtaga taggcaagag cggtgccaga cgcggatgca aaccgaagaa ggaatcaaga
     1921 ggcagaaagc cattcttacc agccttggcc tggggctgaa tggccaggct gggtctgaaa
     1981 cgatagtagt catcgtcacc ataaggcacc agcatattga gagtatcggc cccaccccgc
     2041 aggaaaacca ccaccagcgt gtgcgcgttt gcatgcgcac gcgcgtttgc atatccgtgc
     2101 gcccttgcat tggtaccggg ccttgcctcg gtgcctttcg gctgcggctc ttgccccgac
     2161 tcaaatggct tcagggctaa ctcttgcaaa aatcgacggc gactcttttt catatcaata
     2221 catttgaaag gcggggctag ccagaattag gccaaagagt tcgcggcggt gggcctcctg
     2281 ggcaaactcc tgaaaggcag acaattcctc cggcagggga ttgcgcccgg taaaatatgg
     2341 gaaaagcagt gccggatcag cctttccatc ggcaagagcc gcttccaggc gggccatgga
     2401 attcttcaca ctggcccctt cgataccgcc cagacaaaga gtgagggcaa aattccaacg
     2461 ccagagcaag cttcccagcc agggttcccg ctccaccgga taaccgtcgg gggtcggata
     2521 ttgaaaaggc gcctgcccca tgcgactcaa gaactggcaa agttccttgg aagcatcggt
     2581 atcggccgcc aggctgcgca gggaactgac tacaaagcgg aaagggcgct taaacttggc
     2641 tccgcgcagg catggatcat agaactgctc ggcatcaaat atggtgcgga gcatggccct
     2701 gatatcaccg ccggtatcgg taaatgtctc agccacaagg gcgcataact gaggtggtgg
     2761 ctcatcactg acaaagtgcc tggtaagctt gtaggcaata tagcgagccg tagaaggatg
     2821 ggcgagaact atgtcgagta cggccctcag atctttctcg ccgccaccgg gggcaatgcg
     2881 tttgccgagc accaacttct cgccgtcatc atggcagctc ttgtcaaagt aggcctgacc
     2941 acgcccatac ctatcgtgca aacggaagcc cgaaagagcc cttgccacgt tcaaaacatc
     3001 caactgcgta tagccgccgt ttacccccat ggtgtgcaat tccaaaagtt ctctggcata
     3061 gttctcgttg ggcttttcca tcaagttgcg gcgcttattg tctttaccat cgaggtaaac
     3121 gagcatcgcc ggagagcgcg ccgaagcaag cagtaggtcg gaaaactttc cgagggcatg
     3181 ggctcgcacc acttttctgt catcagtggg cttgagataa atgcaatccc ctttctcaag
     3241 gctgatatta aagtgatcgc tccagaattc caccatcact tccagtaact gacggcggct
     3301 atataccgcc tttaacagag aatgccgcac catgtccgag cgcagggcct ccctcttaaa
     3361 ctcataagcc gtgcccgggt ctatttccag actttcaaaa cgcctcgagc gcaaatcgca
     3421 ggccttatcg tcaatagctt cgggattaag ctgctcttcc aaccaggctt tgcgccctat
     3481 cttcttaagc cgctccagat caccgggata accgccaaag ccggctcggc tcaataagtg
     3541 gaaatccggg tcgatgtcct ttccccaggt acggcagagt ttatcgggaa ctcgctcacc
     3601 caggttctcg gctacggcgt tacgaagcat tgtgcaaccg gacagggata gtcccgcaaa
     3661 agccgccgtc aaaagcacat ccctgcggct tacggtcatc agcttgcaac ctgggacggc
     3721 acttccacat tgacgacggc atcaggcggc gcctgtcgcc tgagagctag taaccctcgc
     3781 acaaaagaac cgtagccaat aacaagggcc gccggcaaga gcaagaacca gcccagtagc
     3841 ggcaaaataa aggaaagcac catcagcagt ccgcctcgca aggtaggcag ccaggggcgc
     3901 acttgatcgc ggggagaagg caagcgccgc cccacaaggg taaccaaccc cgaaagaccg
     3961 atattggcaa agagcaaagc aaaggcaatc gcggcaatgg caagcattcc cccaatgccc
     4021 tcgggcagct tgctgaaaac gcccaccagg atgaccgtaa gcaccaggaa gggtaagccc
     4081 actaaaaaag acttccagag cttgcgggaa gaagcctggc aggaagcctc caccaccccg
     4141 ggccagagac cgagcaaagt cagccagagc gccggcaaag taagaatcag ccccactacc
     4201 acaaaaaata cggccatcgt gtcggctaac atcatggcag cggcttctcc taaatttaaa
     4261 agcgtctttc gtctattata tgcctcaggc aaaccggagc taacaaactc cgccctccta
     4321 acaaattacc gaaagaaagc gccggagcaa aaccgatgtc aggcaagtgg ccgtttcttc
     4381 ttacttcgct tttcctgcaa gccgcccccg accttgtgct ctggagctgg gactaccccc
     4441 aagacttgag cttcttgaaa aacgagaagg ccc
//
LOCUS       DAOQNZ010000121         4456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8555_length_4456_cov_2.965235, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000121 DAOQNZ010000000
VERSION     DAOQNZ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8555_length_4456_cov_2.965235"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="PKW73_03260"
     CDS             <1..1740
                     /locus_tag="PKW73_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNA72327.1"
                     /translation="QHGKVSGAQRPLAFGDFAVLVQRNRDFAQFEQVFASLGIPFVVF
                     GGSGFLRRQEVLDFESLLRFLDKPQDSHSLLAVLRSPFCALSDDLIHRIGAAGDGPLY
                     ARLQTMAAGSAPPAVAKAAQLLRRLLDEAALLPLSELVHKIVSGTGYELAMLAAPDGR
                     QRSRNVWKLVHLACQDDHLSCGEFADKLSAMREFNMRESEAPLDSGDSVKLMTVHASK
                     GLEFAAVALPCLGAAALQNSARSIFHPGYGIAFNTKRLEEEEIPAWYQVAGYLDRQME
                     WEERKRLLYVAMTRARDFLALFMKAEGRKVQSYRTMLRTVLSLDGDHTVLPEPGSALR
                     LSLPFSSALVPYSLRFAQSARRSARVAPELQLAKPSDLLLAPLPVHIEYPKVNELGLS
                     RITSPAATKDALLSPLSAGPELFSPSFLGVFFHALMENLPTYSKADAAALYVYVRDIA
                     STQSFHMAHGPKLERLVTEGLRLLDIYFESRLHQLLSTASRRYNEAEYSLFHGDDLIK
                     RRPDLIFQTFDGDWYLVDFKTDHAAGEVEMRARAGRHSVQLNNYRAELSRLSGLSLTA
                     HIYFAQTGILFPV"
     gene            complement(1752..2384)
                     /locus_tag="PKW73_03265"
     CDS             complement(1752..2384)
                     /locus_tag="PKW73_03265"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA72328.1"
                     /translation="MKAAPLLLSALMVLSLSGCAKKQAPTAYGRWGYLDKTGKFLILP
                     QFDGAAEVTDNGAVVLQNKHLMRLDPETLKESSTPIGPDDKAELPPLTELRFAEAGTD
                     TYKIKDGETVIFDPTEKKKELPSVFPKNGQACARFGDKFAFITPEGKLGAGIFDEARP
                     FHEGRAAVKQAGKWGYIKKDGSFIIPPQYLEAGSFNHGLAPVKQQQDPPN"
     gene            2554..4392
                     /locus_tag="PKW73_03270"
     CDS             2554..4392
                     /locus_tag="PKW73_03270"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA72329.1"
                     /translation="MADDVERAAVSPGIASSPWSGRRATLVILALNVGVFLAMCTFTS
                     FDNFLRTSTATAIEWGASYGPDTLTGQPWRLLTSSFLHFGFFHLASNMLVLWLLGSQV
                     ERMYGTWRFILIYMVSAVGAGCLSLLAVPTGAGAGASGAIYGLAGALLVFAITEPWPV
                     VRVLLTRTGIGGLIYAAISFVLGIGGGAQVANHLGGLFSGFLIGYFCRRDLRSRNRLE
                     KMATWAWVVSLLVGLFVVVTYAPFDIKGNYKLALAVHELDQGRARAALAICDEFLERN
                     PKSGSGILYRGLSRLALKEDEAALADINAAIKQGAELKAALGARMGAAFDLGYYRQVI
                     SDVTELLELGGDVNCIYLRAMACAQLHEFDEAFADARKLTRVFSRKADGAILSAEILR
                     AQGRLKEALTEIGEALQADPDLKRALHLKVVLLAEEGSSEGGTSLQESLDLLGRLGRD
                     RSQPRQFVYYAMIRLGLGDYEGAVKDADVFLAQEDAWKSGKATYPLIIKLLALQALHR
                     EDAQRSLALQVKKKLSEHCWPYPLLSYLLGWTSLSELTGESNNLSRQTETDFYLAVSD
                     FEGGRKTAARERLDKVVERGNLTYMEYEVGRLLRARLLKDSISTGK"
BASE COUNT          870 a         1177 c         1310 g         1099 t
ORIGIN      
        1 cagcacggca aagtctccgg cgcccagaga cccctggcct ttggagactt tgccgtgctg
       61 gtgcagcgca acagagactt tgctcagttc gagcaggtct ttgcttccct ggggattccc
      121 tttgtggtct ttggcggtag tggctttttg cggcgccagg aagtgcttga ttttgagagt
      181 ctgctgcgct ttctggacaa gccgcaagac agtcattctc ttctggctgt cttgcggtcg
      241 cctttctgtg ccctcagcga tgatctgata catcgcatcg gtgcagccgg tgatggacct
      301 ctctatgcca ggctccagac catggccgcc ggctccgctc caccggcggt ggcaaaggct
      361 gcccagctct tgcgacgact tctggatgaa gcggcccttc tgcccctgag cgaacttgtg
      421 cataagatcg tttccggcac gggctacgaa ctggccatgc tggcagctcc cgacggcagg
      481 cagcgttcgc gcaatgtctg gaaactggtg catctggcct gtcaggacga ccatctcagt
      541 tgcggcgagt tcgcggacaa actgagcgcc atgagggagt tcaatatgcg tgaatcggaa
      601 gcgccgctgg atagcggtga ttccgtcaag cttatgaccg tgcatgcctc caaaggtctg
      661 gagtttgcag cggtggcctt gccctgtctg ggtgctgctg ctctgcagaa cagtgctcgc
      721 tccatctttc accccggcta tggtattgcc tttaatacta agcgcctgga agaggaagag
      781 attccggcct ggtaccaggt ggccggttat ctggaccggc aaatggaatg ggaggagcgc
      841 aagcgtttgc tctatgtagc tatgaccagg gcgcgtgact tcctggctct tttcatgaag
      901 gcggaagggc gcaaggtgca gtcttaccga accatgttgc gtacggtgct ctcgctcgat
      961 ggcgatcata ctgttcttcc ggagccgggc agcgctttgc ggctcagcct gcctttctcc
     1021 tccgctctag tgccctacag cctaaggttt gctcagtctg ccaggcgctc tgccagagtg
     1081 gcgccggaat tgcagctcgc caaaccttca gacctcttac tggctccttt gcccgtgcac
     1141 atcgagtatc ccaaggtgaa tgaactgggt ctgagccgta tcaccagccc ggctgcgacc
     1201 aaagatgctc tcttgagtcc gctcagtgcc ggacctgaac tcttctcccc cagtttcctg
     1261 ggcgtctttt ttcatgcctt gatggagaac ctgccaacct atagcaaggc ggatgccgcg
     1321 gcgctctacg tctatgtgcg tgacattgct tccacccaga gttttcatat ggcccatggt
     1381 ccgaagctgg agcgactggt gaccgagggg ctgcgcttgc ttgatattta ctttgaaagc
     1441 agacttcatc agcttctcag cacggcaagc cgccgttaca acgaggcgga gtactcgctt
     1501 tttcacgggg atgacctgat aaagcggcgt cccgatttga tttttcagac ttttgacggt
     1561 gactggtatc tggtcgactt caagaccgac catgccgcag gcgaggttga gatgcgggcc
     1621 cgcgccggtc gccacagtgt tcagctaaat aattaccggg cagaactttc cagactgtcg
     1681 ggtctcagcc tgaccgccca catatatttt gctcagaccg gcatactctt ccctgtctaa
     1741 ggcggctaag gctaattggg cgggtcttgc tgctgcttga ccggagccag accatgattg
     1801 aaggaaccgg cttccaggta ctgaggcgga atgatgaagg aaccgtcttt ctttatgtag
     1861 ccccatttcc cggcctgttt aacggcggcg cggccttcat ggaagggtct ggcttcatcg
     1921 aaaatgccgg cgcccagttt gccttcgggc gtgatgaagg caaacttgtc gccgaaacgt
     1981 gcacaggctt gcccgttctt cggaaacacc gagggtagtt cttttttctt ttcggtagga
     2041 tcgaagataa cggtctcgcc gtctttaatt ttataagtgt cggtaccggc ctcggcgaag
     2101 cgcaactccg tcaagggggg cagttcggcc ttatcatcgg gaccgatggg agtggacgac
     2161 tctttcaagg tttcgggatc aaggcgcatt aaatgcttgt tctgtagcac tacggcgccg
     2221 ttgtcagtca cttccgccgc tccgtcgaac tggggcagga tcaggaattt gccggtctta
     2281 tcaagatagc cccatcttcc ataagcagtg ggagcttgct tcttggcaca accagagagg
     2341 gaaagcacca ttagggccga gagtaaaaga ggagcagctt tcatatttat cctgacaggg
     2401 gcaaccggtt tgtacgacta atctaaaaag gatagcgacg tttgcctgaa aaatccagtg
     2461 atcgtgattt gtatgtccga cgtcggctgc atttcttgta aatctgtggc aggataagca
     2521 gatgagttgt aaggattctg aggagtttgt ggaatggcgg acgatgtcga aagggcagcc
     2581 gtaagcccgg gcattgcctc gagcccctgg tccggccgtc gtgccaccct ggtcattctg
     2641 gcgttgaatg tcggggtctt cctggctatg tgtactttta catccttcga caacttctta
     2701 agaacctcta ctgccactgc cattgaatgg ggggccagtt atggaccaga caccctgacc
     2761 ggtcagccct ggaggctttt aacttccagc tttctgcatt tcggcttctt ccacctggcc
     2821 agcaatatgt tggtgctctg gctgcttggt tcgcaagtgg aacgcatgta cggcacctgg
     2881 cggtttattc tcatttacat ggtttcggcc gtaggagcag gttgtctcag ccttctggcg
     2941 gtaccaaccg gggccggtgc cggggcttcc ggggccattt acggtctggc cggtgcctta
     3001 ctggtattcg ccataaccga gccctggccc gtggtgcggg tgcttctgac gcgcacgggt
     3061 atcggtgggc tcatctatgc cgccatcagc tttgttcttg gtattggtgg cggtgcccag
     3121 gtagccaatc atctgggtgg gcttttcagc ggctttctga ttggctattt ttgccggcgc
     3181 gatctgcgca gccgaaacag attggaaaaa atggcaacct gggcatgggt cgtttccttg
     3241 ctggtggggc tgtttgtggt agttacctat gcgcctttcg acatcaaggg gaattacaag
     3301 ctggcactgg cagtgcacga actagatcag gggcgtgccc gagcggctct cgccatctgt
     3361 gacgagttcc ttgagcgtaa tcccaagagc ggcagcggca ttctctatcg aggcttgagc
     3421 cgcctggcac tgaaagaaga tgaggctgcc cttgccgata taaatgcagc cattaagcag
     3481 ggcgctgagc tgaaggcggc tcttggggct cgtatgggag cggcctttga tcttggctac
     3541 taccgtcagg tgatcagtga tgtaaccgag cttttggagt tggggggcga tgtaaattgt
     3601 atctacctgc gggccatggc ttgcgcgcag ttacacgaat tcgatgaagc ctttgccgat
     3661 gctcgcaaac tgacccgagt tttttccaga aaggctgatg gtgcgatttt atcggcggaa
     3721 atcttgcgcg ctcagggccg gctcaaggag gcccttactg agataggcga ggctcttcag
     3781 gccgatcctg atttaaagcg agccctgcac ctgaaggtgg tcttgctggc cgaagaggga
     3841 agcagcgagg gcggcaccag cttgcaggag tccctcgacc tattaggccg cttgggccgg
     3901 gatcgctccc aaccccggca gtttgtctat tacgccatga ttcgccttgg actgggtgat
     3961 tatgagggtg ccgtcaagga tgccgatgtt tttctagccc aggaggatgc ctggaagagc
     4021 ggcaaagcca cctatcccct cataatcaag cttctggctc tccaggctct tcaccgggag
     4081 gatgctcaga gaagtctggc tctgcaagtg aagaagaagt tgtcagagca ctgctggcct
     4141 tatcctctct tgagttatct gctgggctgg accagccttt ctgaacttac cggcgagagt
     4201 aacaatttga gtcgacagac tgaaactgat ttttaccttg cggtttcaga tttcgagggc
     4261 ggacgcaaaa cggcagcccg agagaggctt gacaaggtgg tggagcgtgg caacctcacc
     4321 tatatggagt atgaggtagg gcgtctcctg cgtgccagat tgctcaaaga ttcaataagc
     4381 accggaaagt aaatcatgac tccgtagaga agtgattggt gcgcaggata tcccgcaagc
     4441 ggcggttaaa agcctc
//
LOCUS       DAOQNZ010000122         4453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8567_length_4453_cov_4.019554, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000122 DAOQNZ010000000
VERSION     DAOQNZ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8567_length_4453_cov_4.019554"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1391)
                     /locus_tag="PKW73_03275"
     CDS             complement(126..1391)
                     /locus_tag="PKW73_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNA72330.1"
                     /translation="MTEVISDVDSKTMTPTLVYQGSVKDVYELGSEELLFHFSDDFSV
                     FDWGKMPDRISGKGRALTLFAAGFFEKMTGASFWQELPNSKHLQQFDRHYLQTIFSAL
                     EPSETLANHYRGLYSRGKTFKLSAPALADQDQSQELYLQVARARVDRPEVRKIGQSLV
                     YFYDAAARGEGLSFIPLEVVFRFAAPAGSSLLSRLEKDPNYAYVLGLEPDAVKVGALF
                     PHPVIEFFTKLEPSDLLLSYQEAAAIAGLSPAEFERLYQTAHLCALAIFHVFAEQGIT
                     LLDGKVEAAVERRLSGKGEMTSRIVLADSIGPDELRLDYKGIQLSKEVLRRYYRNTAW
                     YREIESSKAEAAKRPSVKWQDICRHEKGSAPPPLPPALKECIANMYLALAATMVGGPL
                     AEKICPLSLPAVAEQLAKEIDLAEKEGKP"
     gene            complement(1388..2887)
                     /gene="purF"
                     /locus_tag="PKW73_03280"
     CDS             complement(1388..2887)
                     /gene="purF"
                     /locus_tag="PKW73_03280"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNA72331.1"
                     /translation="MCGIVAISGSTCASSEVLLGLMNLQHRGQDGAGILSLDEATETG
                     GKALHFSLSKQAGLVSLGELEESVRHKKNSYALGHTRYATVGGDGVEMLQPFLIEKFG
                     IGLAHNGNIVNFLDMEDELARDSALRLSSGRWVLSDSFMLLAQLARTLRWRDWNSSAI
                     FDAVRSVMRKASGSYSVVSILENGTIFGFRDPHGIRPLFYGKRTGPERTEYGVASEPV
                     ALSYLGFKEVVEVKAGEAFVISPDGKFESALIGSGKEYAPCMFEWVYFSRVESEFGGK
                     SVYDARFALGVELGKTVKAMGLQADVVVPVPETSRVSAIALAEYLGLPFREILVKNRY
                     VNRTFILDNQTSREEAIRRKLFPIASELQGKRCLIVDDSIVRGNTARQITKLVRDSGA
                     TDVTLLSACPSIKHPCFYGIDFPDKNELLAGHLNEEEVRQELGADRVIFQSLEGLTNA
                     LGSQKLCTGCLTGQYPTDVTIAGRFAAKRKVDREAKPEAEKAVVSRKSK"
     gene            complement(3218..3862)
                     /locus_tag="PKW73_03285"
     CDS             complement(3218..3862)
                     /locus_tag="PKW73_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNA72332.1"
                     /translation="MSTKEYIEPEALLRGLSETIRQRRKVLKISQEDLSRSAGFARGY
                     LSDIERGARSFSIKNLARLSNVLKTCPSALLSTTEKRLNGSSVFADFRLDLPLIGALL
                     GKPDRALAIVDAKQADFPLIFVSDTFSQIFGYDRDEAIGRNFSFLLRGELYQEGLKTV
                     CQAVENKTSCVATLKIYCKDETVLMGEVSSSPVLDDKGEVLYVVCLYQGAAVSE"
BASE COUNT         1129 a         1248 c         1119 g          957 t
ORIGIN      
        1 ccgggactga caaaaacatg cttgacgcgg gggctgcggg ccagtttcca ggccagggca
       61 tgctcccggc cgccgccgcc taccaccagc accaattcct gcaattgctc tttcgtgtcc
      121 accatttacg gcttgccttc cttttctgca agatcaatct ctttcgccaa ctgctcggca
      181 actgcaggca gggacagggg acagatcttt tcggccaggg ggccgcccac catggtggcg
      241 gcaagggcca gatacatatt agcgatgcac tctttcaagg cgggcggcag aggcggcggc
      301 gcgctgcctt tttcatggcg gcaaatatcc tgccacttca cagatggtct cttggctgct
      361 tccgccttac tgctttcgat ttcccggtac caggcggtat tgcggtaata gcggcgcaaa
      421 acttcttttg agagctggat gcctttgtaa tcgaggcgca attcatcagg cccgatactg
      481 tcagccagaa cgatgcggct ggtcatctcg cctttgccgc ttagcctgcg ctcgacagcg
      541 gcttccactt tgccgtccag taaagtgatg ccctgctcgg cgaaaacatg gaaaattgcc
      601 agggcacaaa gatgagcggt ctggtagagc cgctcgaatt ccgccgggct caaaccggct
      661 atggcagcgg cttcctgata agaaagcagg agatcggagg gctccagctt ggtgaagaac
      721 tcaatcaccg gatgagggaa aagagcccca accttgacgg catcaggctc cagtccaagc
      781 acataggcat agtttggatc tttttccagt ctggaaagaa gcgaacttcc agccggggcg
      841 gcaaagcgaa aaaccacttc cagggggata aaactgagac cttcgcccct ggcggcggcg
      901 tcgtagaagt aaaccagaga ctgcccgatc ttgcgcacct ccggtcggtc cacccgcgca
      961 cgggccacct gtagatataa ttcctgggac tggtcctggt cggcaagggc cggcgccgac
     1021 agcttgaagg tcttgccccg actataaaga ccacggtaat gattagccag tgtctctgaa
     1081 ggttctaagg cactgaagat agtctgcaaa tagtgccggt cgaattgctg aagatgcttc
     1141 gaattcggca attcctgcca gaaagaagca ccggtcatct tttcgaaaaa accggccgca
     1201 aacagggtga gagctctacc tttgccgctt atccggtcgg gcattttgcc ccagtcgaag
     1261 acggaaaagt catcggagaa gtggaagagt aattcctcag aaccgagctc atagacatcc
     1321 ttgactgaac cctgataaac aagagtgggg gtcatggttt tgctatccac atcggagatg
     1381 acttcggtca tttgctcttc ctcgaaacta ccgctttttc tgcttccggt ttggcttctc
     1441 tgtccacctt gcgcttggcc gcaaagcgcc cggcgatggt aacgtcggtg ggatactggc
     1501 cggtcaaaca gcctgtgcaa agcttctggg aaccaagggc attagtgagg ccctccaggc
     1561 tctgaaaaat aactcggtct gcacccagtt cctgcctcac ttcttcttcg ttcagatgac
     1621 cggcaagcaa ttcgtttttg tcgggaaaat cgatgccata gaaacaagga tgcttgatgg
     1681 aaggacaggc tgaaagcaaa gtcacgtcag tggctccgga gtcgcgcacc aacttggtga
     1741 tctgccgggc ggtattgccg cgcacaatgc tgtcatccac aatcaggcaa cgtttgccct
     1801 gcagctcact ggctatgggg aagagtttcc ggcgaatcgc ttcttcccgg ctggtctggt
     1861 tgtctaaaat gaaagtacga ttgacgtagc ggttcttcac cagaatctcg cggaagggta
     1921 gacccaggta ttcagccagg gcaatagccg aaactctgct ggtctcaggc accggcacaa
     1981 ccacatcagc ttgtagcccc atggctttca cagtcttacc aagttcgacg cccagggcaa
     2041 atcgagcatc ataaaccgac ttaccgccaa actccgactc cacccgggag aaatagaccc
     2101 attcgaacat gcatggagcg tactctttac cggaaccaat cagggccgac tcaaattttc
     2161 catcgggact gatgacaaag gcttcccccg ctttcacctc cacgacttct ttaaagccca
     2221 ggtaggagag ggccaccggc tcactggcca cgccgtactc agtacgctcc gggccggtac
     2281 gcttgccgta gaacaagggt ctgataccgt gggggtcgcg aaaaccgaag atggtgccgt
     2341 tctccagaat cgatacaaca ctgtaactgc cggaagcctt gcgcataacc gagcgcactg
     2401 catcaaaaat ggcgctacta ttccagtcgc gccatcttaa agttcttgcc agttgtgcca
     2461 ggagcatgaa ggaatcggag agaacccagc gaccggagga aagcctcaaa gccgagtccc
     2521 tggccagttc gtcttccata tcgagaaagt tgacaatatt gccgttgtga gcgagaccga
     2581 tgccgaactt ctcaatcaga aagggctgca gcatctccac cccgtcacca cccacggtgg
     2641 catagcgggt atgaccgaga gcataactgt ttttcttgtg acggaccgat tcttccagtt
     2701 ctccaagaga caccagaccg gcctgcttgc tgagagaaaa atgcagggct ttaccgcccg
     2761 tttccgtggc ttcatccagg gagaggatac cggccccgtc ctgtccgcga tgttgcaggt
     2821 tcatcagtcc caggagcact tccgacgaag cacatgtgct tccagagatt gccactatcc
     2881 cacacatatt gatactcccg gtttgcttta caccaaacgg atctgccggg acacaccaat
     2941 cccggcgtcg ccacctctta tccaagatcg gtaacgatcc tctctaggtt acgagaagaa
     3001 agaggaaatt ccaagccaat gcgaaaaaat gtcaggtaaa ttcccacaca tctttacatg
     3061 actgcctcaa cttggcgcgg cttccccatc aaagagccgc tggtaagcaa aaaaaagact
     3121 gcctgaacaa acagtagttt gcacttggca aaaccggcga aaatcatcca ccctgcagac
     3181 tttccaaatt aaattcagct tgcgagcaag accaggctta ctcggaaaca gcagcgccct
     3241 gataaaggca gaccacgtaa aggacctcgc ccttatcgtc cagaaccggc gaactggaaa
     3301 cttcgcccat gagcacggtt tcgtctttac agtaaatctt cagggtagcc acacaagagg
     3361 ttttgttttc tacagcctgg cagacagtct tcaggccttc ctgatagagt tctccgcgca
     3421 aaagaaaact gaagtttcga ccaatggctt catctctgtc gtagccaaag atctgagaga
     3481 aagtatccga tacgaaaatc aaggggaagt cggcttgctt tgcgtctacg atcgcaagag
     3541 cccgatcagg ctttcccaga agcgcaccta taagcggcaa atcgagacga aaatcggcaa
     3601 atacactgct gccgttcaga cgcttctcgg tagtggataa gagggcggag ggacaggttt
     3661 taagaacgtt ggacaggcgc gccagatttt tgatgctgaa gctgcgcgca ccacgctcga
     3721 tatcactgag ataaccgcgg gcaaaaccgg cactacgaga aaggtcctcc tgactgattt
     3781 tcaatacttt tctacgctgt cggatagttt ccgacaagcc cctcaaaagg gcctcgggct
     3841 caatatattc ttttgtgctc ataaattcgc ctttcaaaag tggcacaacg agaaacaaaa
     3901 gtgcgtccgg aggagaaccg caagggcttt tccgtcaagc tatatatttg ctcgaaattc
     3961 gcactctcag cgcttcccac caagaaaacg cctcgcagta ccaagcaccc agccaaccaa
     4021 tcatacaaaa atcagacttt gactaagcta agagtaaaaa tccaacgtat gagaatcacc
     4081 atattcccag tataggtcag ggtgcttaag tcagcaaggc tcggcgttgg ccaccatttt
     4141 cggcagaaac caagtggtta aaagtaagcc tcgctcccca tgcggaaact gcggctaagt
     4201 ggcggaacat catccctaca caaaagaccc gggccggttc tgcattctct gcggcgaaaa
     4261 gtcaggccgg caatgggcgg aagccctttt gttcagtccc ataataatgc tcgtagtgaa
     4321 actacacact cctcttgacc cgtgaattca gctaccggaa gctaagcggt agatagaatt
     4381 aattcgctta gatgcaaact ctcccctcaa accacaagag gaaccgccgc cagggcagtt
     4441 cctcttgctc ata
//
LOCUS       DAOQNZ010000123         4450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8579_length_4450_cov_3.577474, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000123 DAOQNZ010000000
VERSION     DAOQNZ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8579_length_4450_cov_3.577474"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..727
                     /locus_tag="PKW73_03290"
     CDS             182..727
                     /locus_tag="PKW73_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72333.1"
                     /translation="METLTNTSIWQSAWLMPIIYSLFIAGSFFLGIFLTAVKQRPYFQ
                     ANSNNFWLVILAVLFAAGYISVTYFGSSPVWALLAFGIGAFVKFSEITTEAARRNFMK
                     ERAAAIERIKVVKEELQARTSDEEIVLFEISSWSGFQTSAVIQFALASGLTVVVESNY
                     SDQTVHLKLSGALERDLELGS"
     gene            765..1706
                     /locus_tag="PKW73_03295"
     CDS             765..1706
                     /locus_tag="PKW73_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72334.1"
                     /translation="MNRPNPSSTQSRNLISVASALLFVVVLKFALGLSWFAATGLTTL
                     IIIHEFGHLVTIWHYGIKCTPPMFMFPFGALITLEEPVKEESTEAWIALGGPILGTMG
                     ALLPLPVYLMTGDKEWLSLVEMGLVINLLNLLPFGTLDGGRVIGAIMPGIRGLLVVLA
                     LVAAVVLNWSILFLIACFSALELPKENRWRANLILGTVIFATAQVFYQPGSLSLLVAV
                     VVWFLTLPHLRNSQMLPPTTSQEAAVTSEEQAVQDGEAAEDEPQEEPEQQTVPLQIGR
                     VKASRNLAIFIGMLYFGLVLALLQGLYLTALVLHSGS"
     gene            complement(2075..2434)
                     /locus_tag="PKW73_03300"
     CDS             complement(2075..2434)
                     /locus_tag="PKW73_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72335.1"
                     /translation="MRLLVGGFLWMKGIFAVLVKYLDRSASESRNSMMPSSVLSARLQ
                     KLQAERESLEWQLAIAEELGVAPDTLNALQAQIEQLELPKVALPVPPQSSPAQTEAPA
                     CLILESMDDVKAVQAPG"
     gene            complement(2574..2771)
                     /locus_tag="PKW73_03305"
     CDS             complement(2574..2771)
                     /locus_tag="PKW73_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72336.1"
                     /translation="MAERKLEVLKAFFPQSAARSFHVTCEVGDILIIEQEYDHGTMCK
                     KNGVMCFLLEEEVYRYCRELS"
     gene            3447..3842
                     /locus_tag="PKW73_03310"
     CDS             3447..3842
                     /locus_tag="PKW73_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72337.1"
                     /translation="MSILIPIALLLGIYALYSVMTAPQRARANSNRQADDQLQRAASF
                     LLTGRLDEAEAAYFSALRAARASGTPIYEAEANSGLADVARRRGQLEQARTYLQAALV
                     LRPQFEQYYPNYGPLLERELAEVEEELRV"
     gene            3992..4414
                     /locus_tag="PKW73_03315"
     CDS             3992..4414
                     /locus_tag="PKW73_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72338.1"
                     /translation="MPPLRPSKPAKKPPKTSSANNGVIPMRATTNSALSDLRIFYSTG
                     RDDTNSILVTGLPGDEGFEPTDNCYFRTAASLRWEVNRLIREKAHACGLGHRLVNTEK
                     YIGTTGWTFYGQGQAASVRSIALALAAHFQVDLLDKSA"
BASE COUNT         1071 a         1305 c         1085 g          989 t
ORIGIN      
        1 ctttaccaag ctggaagacg gcagtcgcgg catccgtgaa tacatcatca ccaatagatc
       61 ggtgcaagag ctggaagact acgggctctt caccctggat gtaaatctgc cgacggcgat
      121 gcccttaacg tcggcttgaa attcgtcacc caacaaccac aacaacaaac ccgggagcct
      181 tatggaaacc ctcactaata caagtatctg gcaaagcgcc tggctcatgc cgataatcta
      241 ctcactcttc atcgccggca gttttttcct cggcattttt cttacggcag taaagcagcg
      301 tccgtatttc caggccaaca gcaataactt ctggctggtc atactcgcgg tactctttgc
      361 cgccggttac atctctgtga cttacttcgg cagttcaccg gtctgggctt tgcttgcctt
      421 cggcatcggc gcctttgtca aattctcaga gatcactact gaggcagccc gtcgcaattt
      481 catgaaagaa agagcggcag ccatcgaacg tatcaaagtc gtcaaggaag aactgcaggc
      541 tcgtaccagc gacgaggaaa tcgtcttatt cgaaatctca agctggagcg gctttcagac
      601 ctcggcggtg attcagttcg ccctcgcctc aggtctgact gtggtagtcg aaagcaacta
      661 ctcagaccag accgtccacc tcaaactaag cggcgccctg gaaagggacc tggaactagg
      721 ttcttaagcg ccccgacaac cttctactct tactggaatt cattatgaat agaccaaatc
      781 caagtagtac tcagtcacgc aatttaatct ctgttgcctc ggcccttctc tttgtcgttg
      841 tcttgaagtt cgccttaggc ctttcctggt tcgccgccac cggtctgaca accctgatca
      901 tcattcacga attcggtcac ttggtcacca tctggcacta cggcatcaaa tgcacgccgc
      961 ccatgttcat gtttcccttc ggagccctga tcacactgga agaaccggtg aaggaagagt
     1021 ccaccgaagc ctggatcgct ctcggcggac caattcttgg aacaatggga gccctcttgc
     1081 cgttgcccgt ctacctcatg accggcgaca aagaatggct ctccctggtg gaaatggggc
     1141 ttgtcatcaa cctgctcaac ctcttgcctt tcggcacact ggacggcggt agggtcatag
     1201 gcgcaattat gccgggcatt cgcggactgc tagtggtact ggcactggtg gcagcagtcg
     1261 tcttgaactg gtccatcctt ttcctgattg cctgcttctc ggcgctggag ttgcctaaag
     1321 aaaaccgctg gcgcgccaat ctcattctgg gcacggtcat ttttgccaca gcccaggtct
     1381 tctaccaacc aggctcgctt tccctgctgg tagccgtggt ggtctggttc ctcacactgc
     1441 cgcatttgag aaattcgcaa atgctgccgc ccaccaccag tcaggaagcc gctgtaactt
     1501 ccgaagaaca agcagtacaa gatggtgaag cagcggaaga tgagcctcag gaagaaccgg
     1561 aacaacaaac cgtgccctta caaataggca gggtaaaagc cagccgcaac ctggctatct
     1621 tcataggcat gctctacttt ggtctggtgc tggcactctt gcaaggactt tacttgactg
     1681 ccctggtctt acatagcggc tcataacagc cgcacttttg gagacgatgc cctcgatgag
     1741 gtgcatcgtc tctctttttc cttgctaatc aaggaagctg gagaccgact atttcattct
     1801 tggggcttgt ttttcatgaa tgctcagccc tgcaagtttc tttgttccgg cgccttacta
     1861 cattctataa tatcttaagg gcaacaataa aaaatccagg agttatctat gcttaaggca
     1921 ctgcctgcat caaatacaca ggacagcaaa aaatggttta ccgttgtttg ccgcgacgat
     1981 agccactgcc ctcaacaaaa gcaaaggaaa tacccatgta agctcacatc tgatacctac
     2041 atgggcgaag gaaattacta ccgattgtct ctgcttagcc tggcgcctgc actgccttga
     2101 cgtcgtccat cgactctaaa atcaagcagg caggtgcttc ggtctgggca ggggaagact
     2161 gaggcggcac cggcaaggca actttgggaa gctctaactg ctcgatctgt gcctgcaagg
     2221 cgttcagtgt gtcaggtgcg acacccaatt cctcggcaat ggccagttgc cattccagac
     2281 tctccctttc cgcttgcagt ttctgcaacc tggcagaaag tactgaagac ggcatcatcg
     2341 agtttctgga ttctgaggcg gagcggtcca agtacttgac cagaaccgca aagatcccct
     2401 tcatccagag aaaaccccca actaacaatc tcacgctcaa ctccacccgg gcatcccgga
     2461 gagagaaaaa agacgcgcag ccgacaagca cgccttgccg gacttatgcg ctctacctga
     2521 ggcagtaaca agagagaggg gggtgcgccc gggcgcgaca caccccacca aactcagctc
     2581 agctcccggc agtagcggta cacctcttct tccagcagga agcacatgac gccattcttc
     2641 ttgcacatgg tgccatggtc gtactcctgc tcgatgatga gaatgtcgcc gacttcgcag
     2701 gtcacatgga aactgcgggc tgcggactga ggaaagaagg ccttgaggac ctcaagcttg
     2761 cgctcggcca tgttttgctc cttacgacca ccatggtcgc attgttcaca ccgaaggaaa
     2821 cttgagacaa gcccatccgt ccagccccat gcaatgcttt catctgccgg ttcgaagtga
     2881 gcgctcggcg catcccctga gtggcgacaa aagcaaggaa gatgaggtct tgaagactga
     2941 ttgaagactc gctgtagttt cctgaaaagt aaaataaata agaactctct ggtattaaca
     3001 tttgcctctt cgaataaaca tccgtacaag tgttaattag ctccaataca gcgccgtcat
     3061 cggcagcagc agcgcggcag caaggcgaaa agccaggccg gacaaggctt gacacgactg
     3121 cggcggaggc tcaccgcagc cgataagcgc gctctaatgg gtttagcgtc tcacgcagct
     3181 tccgtaagca agcgagaaac ggccaaaagt cctccagcat tggcgaaaac ttagggaagg
     3241 agcggttgag ccgccaagca ggggttagct aggctgaaga cccaatttct tacactaaat
     3301 cagcccctca gattttctct acaccagaac cttagcgcaa acaccaacct tcatccgtat
     3361 ttcagggtga ccgaaggtaa ccgcgctggc ttctgagatt gaatttgagc gactgcttct
     3421 ttccactcat aacagacagg tagcccatgt caattcttat accaatcgct ctccttctgg
     3481 gtatctacgc tctctactcc gtaatgacag caccccagcg tgcccgtgcc aacagcaacc
     3541 ggcaagccga cgaccaactg caacgtgcag cctccttcct gcttaccgga cgccttgacg
     3601 aagccgaagc ggcctacttc tctgctcttc gcgcagcccg tgccagcggt actcctatct
     3661 acgaggcaga agccaatagt gggttagccg atgtggcccg ccgccgcggt caactggagc
     3721 aagcccgcac ttacttgcag gcagccctgg tactgcgtcc gcaattcgag cagtactacc
     3781 cgaactacgg tccccttttg gagcgggaac tggcagaagt ggaggaagag ctgagggtct
     3841 gatagctgcg ccaacctgca acctccttta aagagataag gttaaactgg tcatgacatt
     3901 tttatgattg gtttttcata agtctttttg agtcaggaaa ttagcaggca actgaagcat
     3961 agaaaacagg ctttcacctt ttcactccct tttgccgcca ctgcgaccaa gcaaaccggc
     4021 taagaagcca ccgaaaacct caagcgcaaa caacggagtc attcccatgc gcgccaccac
     4081 caacagtgct ctgtccgacc tgcgaatctt ctacagcaca ggacgagacg atacgaattc
     4141 catacttgtg accggtcttc cgggcgacga aggtttcgaa cccaccgaca attgctactt
     4201 ccgcactgcc gcttcgctgc gctgggaagt caatcgtctc atccgcgaaa aggcgcacgc
     4261 ctgcggtctg ggtcatcgcc tcgtcaacac cgagaagtac atcggcacca caggctggac
     4321 cttctacgga cagggccagg cggcatcggt gcggagcatc gcgctcgcac tggccgccca
     4381 ctttcaggtg gacctcctcg acaaatccgc ctgagccact gccgatgcag gggcgcccgc
     4441 aagcgcccct
//
LOCUS       DAOQNZ010000124         4448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8585_length_4448_cov_4.449807, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000124 DAOQNZ010000000
VERSION     DAOQNZ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8585_length_4448_cov_4.449807"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKW73_03320"
     CDS             complement(<1..923)
                     /locus_tag="PKW73_03320"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNA72339.1"
                     /translation="MKALVKNRNSVQVVNTAAPKITREDDVLLQVALAGLCRTDIYAA
                     TGKLKVKDPLVLGHELAGVVEAVGPSVEGIKPGMRVTIDPVLRCRQCHRCKHDLSCTN
                     TGFVGLDADGGFAEQAVVPAYAVLPLDDSLSFLHAAYCEPVAASLAVLKSGIAPAQKG
                     AIIGKNRFSELLQLILDAYGFPTLPVFDLNDAVHKAELADLESQLDYVIETYASSAVL
                     AAMSKAIKPGGIMVLKSRQYEPLQFRLIDFLKKEPVMHVVNYGDFPEALSLLVSGRIK
                     IDNLIDDVYALSNFEKVFERAQDSEAKKPFF"
     gene            complement(937..1470)
                     /locus_tag="PKW73_03325"
     CDS             complement(937..1470)
                     /locus_tag="PKW73_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNA72340.1"
                     /translation="MSFDFNEENKETLALFPELAKMLSDLPNYQVINETLLRGGQPTR
                     DGFTRLRGEGVKTVVNLREETGQILEEEKLVQILGLSYESIPLNPFHRPGHESVLRFL
                     EIVLEPENQPVFVHCLHGQDRTGMMVGIYRMVVDGFDFKTSFNEMLALGFHQEFTYLT
                     ETARSYEGKNHRDLLQK"
     gene            1562..3169
                     /gene="guaA"
                     /locus_tag="PKW73_03330"
     CDS             1562..3169
                     /gene="guaA"
                     /locus_tag="PKW73_03330"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNA72341.1"
                     /translation="MTTSQVKGDKVRYEENALKDAIAILDFGSQYSQLIARRVREHSV
                     YSELLPHSVTAAELKRMNPRGIILSGGPNSCYEEHAPKLDPEIWKMGLPVLGVCYGMQ
                     LMSHDLGGKVEPASVREYGRALLTVVHHGKLFEGLDPAIESWMSHGDSVVDLPEGFAV
                     VGCTADTPVAAIADEERKYYGVQFHPEVVHTVGGKQIIANFALNICGCPQNWTMSRFV
                     EEACKSVKERVGDRRVLLALSGGVDSSTLAFLLHKAIGDQLTCMFIDQGFMRKNEAQW
                     LVETFERDFHIHVHAIDAAERFISKLAGVEDPEQKRKTIGAEFIRVFESESKRLGPFD
                     FLAQGTLYPDVVESAGTKVDPKTGKPVAVKIKSHHNVGGLPEDLKFELVEPFAKLFKD
                     EVRLVGKELGVPEGIVERHPFPGPGLAIRVLGEVTEPRLKTLREADWIIREEIKAAGL
                     YRQIWQVFAVLLPTKSVGVMGDQRTYQNQIVLRAVTSEDGMTADWARLPYDLLATISS
                     RVVNEVPGINRVVYDITSKPPATIEWE"
     gene            3178..>4448
                     /locus_tag="PKW73_03335"
     CDS             3178..>4448
                     /locus_tag="PKW73_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72342.1"
                     /translation="MGAEDNKDKNEAPSEGKEGGHDWMEFARSEGFDFAPPPRDPNKQ
                     QVVISGSELLGHICSRVTLGLILFIAFLAGAWVAGTALHDPDTCWLMAMGRQIFQTGA
                     LPSTDPFSYTFAPFSQALSGQKFVPYQWLSELIFYLVTVPGGLLPLLMLTATCMVTAF
                     LSLPLSVAVRREAPFLLALIAVILGMLSASFHTLVRPQVFSFVFLSVFLQLVHSVRAS
                     SLAGEKKLFPMVLVAAPLVVLWSNMHTGFTTAFSVLLACLIGTTLALVFGKSPVRSML
                     VTFAVSMLGMLLASLVNPFGVGLWLYIPSLFFSATNKFIIELAPLDFAKPIYYPFLLL
                     VAFYAVSFVVGLRLYLRLRKEAASVELTATAGLLLCELIISFLIGTIGTYNAVKTSRL
                     IPFVALMLVAEIAALLGLANLLGGAGKLPAAA"
BASE COUNT          905 a         1165 c         1281 g         1097 t
ORIGIN      
        1 tcgaagaatg gctttttggc ttcgctatcc tgggcccgtt caaaaacttt ttcaaaattc
       61 gaaagtgcat aaacatcgtc aatcaggttg tctattttga tgcgaccgga aacgagtaat
      121 gacagcgctt cgggaaagtc gccgtagttg accacgtgca tcaccggttc tttcttgaga
      181 aagtcgatga ggcggaattg caacggttca tactggcgac ttttgaggac cattatgccg
      241 cccggtttga tggctttgct catggcggca agaacggcgg aggaggcata agtttcaatt
      301 acataatcaa gctgtgattc gaggtcggca agttcggctt tgtgaactgc gtcgttgagg
      361 tcaaaaaccg gtagggtggg aaagccatag gcatcgagaa tcagttgcag caattcggaa
      421 aatctattct tgccgatgat tgctcctttt tgtgccggtg ctatgccgga cttcaagacg
      481 gccagggaag cggccaccgg ttcgcagtaa gcagcatgca gaaaagagag gctgtcatcg
      541 agaggcagga cggcataggc tggaacgact gcctgttcgg caaaaccgcc atcggcatca
      601 agaccgacga agccggtgtt ggtgcaactg agatcgtgtt tgcagcggtg acactggcgg
      661 cagcgcagca ccgggtctat ggtgacgcgc atgccgggct tgatgccttc caccgatggg
      721 cccacggcct ctaccacgcc ggccagctca tgacctaaaa cgagggggtc tttcaccttc
      781 agtttgcctg tggcggcgta gatatcggtg cgacagagtc cggccagggc tacctgtaag
      841 agaacgtcat cttctcttgt tattttcgga gcagcggtat tgaccacctg cacggaattt
      901 cggtttttaa ccagtgcctt catggaagcc tttaatttac ttttgcagga ggtcccggtg
      961 gtttttgcct tcgtagcttc tggccgtttc cgtcaggtaa gtgaattcct ggtggaagcc
     1021 aagggcaagc atctcattga agctggtctt gaaatcgaag ccgtccacca ccatgcggta
     1081 gataccgacc atcatgccgg tgcggtcttg accgtgcagg caatgcacga agacgggctg
     1141 attttcaggc tccaggacga tctccagaaa acgaagcaca ctttcgtgcc cgggtcggtg
     1201 gaaggggttg agggggatgc tctcgtaact caagccgaga atctgcacca gcttttcttc
     1261 ttccagaatt tgaccggtct cttccctgag attgaccacg gttttcacgc cttcgccccg
     1321 caacctggtg aagccgtccc tggtgggttg gccgccccgc agcagagtct cattgatgac
     1381 ctggtaattg ggcaggtcgg aaagcatctt ggccagctcg ggaaagaggg ccagggtctc
     1441 tttattttct tcgttaaaat caaaagacaa gtaaagatga ccccttaaag ttcaccgctg
     1501 ggcgccgcac tgaaagataa tagtctgcca aagccggatg ggcaacactg aggggggcga
     1561 tatgacaacc agtcaagtca agggcgacaa ggtcaggtat gaggagaatg ctctcaaaga
     1621 cgctatagca atcctggact tcggttctca gtattctcag ttgattgcgc gccgggtgcg
     1681 cgagcactcg gtctattccg agcttttgcc tcacagcgtc accgccgccg agctgaagcg
     1741 catgaacccc aggggaatca tactttccgg cggtcccaac agttgctatg aagaacacgc
     1801 ccccaagctc gacccggaga tctggaaaat gggtctgccg gttctgggag tttgctacgg
     1861 catgcagctc atgtcccacg acctgggcgg caaagtggag ccggccagtg tgcgtgagta
     1921 cggccgggcc ctgctcaccg tcgtgcatca tggcaaactc ttcgagggtc ttgacccggc
     1981 catcgagagc tggatgagtc acggcgatag tgtggtggac ttgccggaag gctttgctgt
     2041 ggtgggttgc accgccgaca cgccggtggc ggccatcgcc gatgaagagc gcaagtatta
     2101 cggcgtgcag ttccaccctg aggtggtaca caccgtgggc ggcaaacaaa ttatcgccaa
     2161 cttcgccctc aatatttgtg gatgtcccca gaactggacc atgagccgct ttgtggaaga
     2221 agcgtgtaag tcggtcaagg agagagtggg agaccgccgc gtcttgctgg ctctttcagg
     2281 cggtgtggat agttccaccc tggccttcct cttgcacaag gctatcggcg atcagctcac
     2341 ttgcatgttc atcgaccagg gcttcatgcg taagaacgaa gcgcagtggc tggtggaaac
     2401 attcgagcga gatttccaca tccatgtgca tgccatcgat gctgctgaaa gattcatatc
     2461 caagctggcc ggagtggaag atcccgagca gaagcgtaag accatcgggg cggagtttat
     2521 tcgtgttttt gagagcgaat ccaaacggct cggacctttc gattttctcg cccagggtac
     2581 cctctatccg gacgtagtgg agtcagccgg caccaaggtt gatcccaaaa ccggtaagcc
     2641 ggtggcggtc aaaatcaaga gccatcacaa tgtgggcgga ttgcctgagg acctcaaatt
     2701 cgaactggtg gagccctttg ccaagctctt taaggacgaa gtgcgtctgg tcggtaagga
     2761 actgggagtg ccggaaggca ttgtggagag acatcctttc cccggtcccg gtctggccat
     2821 ccgggtgctc ggtgaagtga ccgagcccag gctcaagacc ctgcgcgaag ccgactggat
     2881 tattcgggaa gaaattaaag cggccgggct ctaccggcag atctggcagg tctttgctgt
     2941 cttgctgcct accaagtctg tgggtgtgat gggcgatcag cgcacttatc aaaaccagat
     3001 tgtcttgcgg gccgtaacct ctgaagacgg tatgaccgct gactgggcgc gcttgcccta
     3061 tgacttactg gctacgattt cctctcgggt agtgaacgaa gtgccgggta tcaaccgggt
     3121 ggtctatgac attacctcca agccgccggc cactattgag tgggaatagc tcccggcatg
     3181 ggcgctgaag acaataaaga caagaatgaa gcgccgtccg agggaaagga aggcggccat
     3241 gactggatgg agtttgcgcg ctcggagggc tttgattttg cgccgccgcc cagggatcca
     3301 aacaagcagc aagtagtaat ctccggctcc gaactattgg ggcatatttg ttcgcgtgtc
     3361 accctggggc tgattctctt catcgccttt ctggccggag cctgggtggc cggaacggct
     3421 ttgcacgacc ctgatacctg ctggctcatg gccatgggcc ggcagatttt tcagaccggt
     3481 gcccttccct ccaccgaccc cttctcttat acttttgcgc cattttccca ggctctgagc
     3541 gggcagaaat tcgtgcccta tcagtggctc tcggagctta tcttttacct ggtgacggta
     3601 ccgggcgggc ttttgccttt gcttatgttg acggcaactt gtatggtcac cgcttttctt
     3661 tcgctgcctt tgagcgtggc cgtgaggaga gaagcgccct tcttgctggc tcttattgcc
     3721 gtgattctgg gcatgttgag tgccagcttc cacactctgg tgagaccgca agtgttttcc
     3781 tttgtgttcc tttcagtctt tttgcaactg gtgcattcgg tgagagcttc ctccctggcg
     3841 ggcgagaaaa agctcttccc catggtgctg gtggccgcac cgctagtggt gctgtggtct
     3901 aacatgcaca ccggttttac tacggccttt agcgtgcttc tggcttgctt gatcggcact
     3961 actctcgccc ttgtcttcgg taagtcgccg gtgcgcagta tgcttgttac gttcgccgtc
     4021 agcatgctgg gcatgttgct tgccagtcta gtaaaccctt tcggcgtcgg tctctggctt
     4081 tatataccgt ctttgttctt ctctgccacc aataaattca ttattgaact ggctcctctc
     4141 gattttgcca agcctattta ctatcccttc ctgttactgg tggctttcta tgcggtcagc
     4201 tttgtagtgg gcttgcgcct ctatctgagg cttcgcaagg aagctgcttc ggtggagctg
     4261 actgccacag ccgggctttt gctttgcgaa ctgatcattt cttttttgat cggtaccatc
     4321 ggaacttaca atgccgttaa gaccagccgc ttgattccct ttgtggcact gatgctggtg
     4381 gcagaaatcg ctgctttgct ggggctcgct aatttactgg gaggcgccgg caaactgcct
     4441 gctgctgc
//
LOCUS       DAOQNZ010000125         4432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8645_length_4432_cov_3.138679, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000125 DAOQNZ010000000
VERSION     DAOQNZ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8645_length_4432_cov_3.138679"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /locus_tag="PKW73_03340"
     CDS             <1..1775
                     /locus_tag="PKW73_03340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72343.1"
                     /translation="SSAQRQTRTSLVKRKRHWLKEKEGHALTINYASHMMTRRLLDTL
                     KENEHYLWRAWSISWGLIFILVLSVNLPNMLHWFLTHFAGLPPSTSPTSLIDSRVLGA
                     LALTPIFTIFVATSPLLVPTGLALAFLATNTWGQLALSLGVLLVGLRLATFFLKPNRI
                     IADSKGLRAALDLGFREISFNSLDWQSLTSITLDRAEHTDPSKWLLTFHAGQNLLQIR
                     LGGMDQTSKLSLFKTIEKYAPQVSRDADVETSLTQGSAQSFTDLWLSSLATPPKREKL
                     APLETGHILKGGQYMIQRQYSAGGQGLTYLAHAVDEMGDTNSEQNCKLIIKESMLPLY
                     VDDEARRKAVERFEAEALLLKSLEHEKVVALVDYFIEDHRSYLVLERIAGLDLRAVME
                     LKEGRLEGPFSAEEVLKLAAQMCDILQFLHGRTPPVVHRDFTPDNLVMDDQGNIKLID
                     FEVAHEHNQIQRTTATVVGKHAYIPPEQLRGKPEQASDLYALGATLYFLNTGHDPEPL
                     SRSRLPELQDGGEPGKERELLSEIIQGLTALEPSKRLTLTELRRKLGLCQLQADDQNQ
                     EAEKQEEKLTIKVPQPESLVRERD"
     gene            1775..3244
                     /locus_tag="PKW73_03345"
     CDS             1775..3244
                     /locus_tag="PKW73_03345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72344.1"
                     /translation="MSNTEIILEYSDPTADTGKKIMLFTSPLWGILAPLAAVASIPMT
                     GFDPMCCYYVFGFFLITAGCLGATIAFADNRIIISKRGLRVPPLLTLFALGRDEIPWT
                     SISSLAVEEDRNFDPTEAQSLVTKQLVLKLKDKDTISLRLHQLKKEQVEQLVLSLSLW
                     LDEKVALPLLEIKEAAGAGGKRLEDKRRKREGDSDLPSDMTFTQLWQSELQSRYSATA
                     FMPLEPDQTIRDGTLKIIRQISFGGLSAVYLCQQNQKELRVLKESVVPLNNKASLRDK
                     AREMFQREAAILIKVEHEQIARVVDFFTEEERTYLLLAHIEGVNLRQLVRETGALPEE
                     RVLSIAASLLSPLRYLHALTPPVVHRDISPENIILEEEDRAVLIDFGAANEFLGTATG
                     TLVGKQCYISPEQFKGKACTASDLYSLGATLYFLITGEDPTPISSSSPAEKRPEISAG
                     TDALVKDLTALEIEDRIKTVEELQARLTPLLSTGVGNLP"
     gene            3387..4313
                     /locus_tag="PKW73_03350"
     CDS             3387..4313
                     /locus_tag="PKW73_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72345.1"
                     /translation="MNRGNGLESATGQDTGDAGRLNLLTASQEQEPVSKQVNKLAQSG
                     ANLTSGGYFVSRLAEQETALAKQTAADPVKALSPATRKEFLAARTDLEAKLGPAVGRV
                     KEQLTQLKLAHPGTIYEYQAPAITGPRSRDEKYFRVKPGATLPAPIQEAMNTKEALNQ
                     ARLTLARTAGNGLGISKSPQQLEAAMHLNNPYLVRSGLANEAGRMELAAMKVQRELHS
                     ELGKAGQNVSRKVLTATAAFGTAFAANKLVDKALFHDSSPGFFTYAADIASPAVLLSR
                     RSLAFKVGVMTLSHALGKGTDLASIRRTSSKG"
BASE COUNT         1141 a         1278 c         1145 g          868 t
ORIGIN      
        1 caagcagcgc acagaggcaa acacgcacca gcctggtcaa gcggaagcgg cattggctga
       61 aagaaaagga agggcatgcg ctgacgatca actacgcatc tcacatgatg acccgtcgtc
      121 ttcttgatac cctgaaagaa aacgagcatt acctgtggcg agcctggagc atcagttggg
      181 gactaatatt tatactagtc ctgagcgtca atttgcccaa catgctgcac tggtttctta
      241 ctcatttcgc cggtctgccg ccaagcacgt cgcccaccag tctcattgac agccgcgttc
      301 tcggagccct ggctctcact cccattttca caatttttgt ggccaccagc cctttgcttg
      361 taccgacagg actggctctc gcctttctcg ccaccaatac ctggggacag ttggcattaa
      421 gcctgggagt gcttctggta ggtctgcgcc tggctacctt cttcctcaaa ccgaaccgca
      481 tcatcgccga tagcaaaggc ttaagagcgg ctctcgatct aggttttcga gaaatcagct
      541 tcaattcgct ggactggcag agcctcactt ccatcaccct ggatcgagcc gagcacaccg
      601 acccgtcaaa gtggctgctc acctttcatg ccgggcagaa cctcctgcag atacgcctgg
      661 gcggtatgga tcaaaccagc aaactatcgc tctttaaaac aattgaaaaa tacgccccgc
      721 aagtaagcag agatgcagac gtggagacca gcctcaccca gggtagcgct cagagcttca
      781 ccgatctctg gctcagttct ctggccactc cgcccaaacg agagaaactg gcgcccctgg
      841 aaacagggca cattctgaaa ggcggacaat acatgattca aaggcaatat agcgccggcg
      901 gacagggctt gacttacctg gcccatgccg tggatgagat gggcgacacc aatagcgaac
      961 aaaactgcaa gctaattatc aaagaatcga tgctgcctct ctacgttgat gacgaggcca
     1021 gaagaaaagc agtggaacgc tttgaggctg aagctttgct tctgaagagc ctcgagcacg
     1081 aaaaggtggt ggctctcgtg gactatttca tagaagatca cagaagctac ctggtacttg
     1141 agcgcatcgc cggactggat ctcagagctg tcatggaatt gaaggaaggg cggctcgaag
     1201 gacctttcag cgcggaggaa gtgctcaaat tagcagcgca gatgtgtgac attctgcagt
     1261 ttctgcacgg gcgcacacca ccggtagtgc atcgcgattt tacgccggac aacctggtca
     1321 tggacgatca gggtaatatc aaactgattg attttgaagt agcacatgaa cataatcaga
     1381 tacagcgcac cacggccacc gtagtaggca agcacgccta tatccccccg gaacagctaa
     1441 gagggaaacc ggagcaagcc tcggatctct atgcccttgg tgccaccctc tactttctca
     1501 acaccggcca cgaccctgag cccctgtcac gttccagact gccggaactt caggacggcg
     1561 gcgaacccgg aaaagaaaga gaactacttt ccgagatcat ccagggactg acggctctag
     1621 aaccgagcaa gcgcctcacc ctgacagagc tcagaaggaa gctaggtctt tgccagttac
     1681 aggcagatga tcaaaaccag gaagcagaga agcaagaaga gaagttaacc attaaagtgc
     1741 cgcaaccgga gagtctggtc agggaaagag actgatgtcc aacaccgaaa tcattctgga
     1801 atactcggat cccaccgccg acacaggcaa gaagatcatg cttttcactt cacccctctg
     1861 gggtatcctg gcgcccctgg cggcagtagc ctcgattccc atgaccggct tcgatcccat
     1921 gtgctgctac tatgtcttcg gcttctttct gatcacggcc ggttgcctgg gcgctaccat
     1981 agcctttgca gacaatcgca tcatcatctc caaacgagga ctgagagtac cgcccctcct
     2041 cactttattt gccctcggca gagatgaaat tccctggact tccatcagtt cgctggccgt
     2101 ggaagaagac cgcaacttcg atcccaccga agcgcagagc ctggttacaa aacaactggt
     2161 gctcaagctg aaagataaag acaccatttc actaaggctc caccagctaa aaaaagagca
     2221 ggtggaacaa ctggtgctct ccctttctct ctggcttgat gagaaggtgg ctctgccgct
     2281 tctggagata aaggaagcag ccggagcggg cggcaagcga ctggaagaca agcggcgcaa
     2341 gcgagaggga gattccgatt tgccttccga catgaccttc acccagctct ggcaaagtga
     2401 attgcagagt cgctattcag ccacagcctt tatgcctctt gaaccggatc agaccattcg
     2461 agacggcacc ttgaaaatca tccgccagat ttccttcggt ggtctttcag cagtctatct
     2521 ctgtcagcaa aatcaaaagg aattgcgggt cttgaaagag tcggtggtgc ctctgaacaa
     2581 caaagcaagt ctgagggaca aggcccggga aatgttccag agagaggccg ccatactcat
     2641 taaagtcgaa cacgaacaga ttgccagagt ggtggatttc tttaccgagg aagaacgcac
     2701 ctacctcctg ctggcccata ttgaaggggt taacctgagg caactggtac gagagaccgg
     2761 agccctgcct gaggaaagag tgctttctat agccgcatcg ctgctctcgc cgctgcggta
     2821 cctgcacgca ttgacaccac cagtagtgca ccgcgatatc agtccggaaa atataatttt
     2881 ggaagaagaa gatcgagccg tgctaatcga cttcggcgcc gccaacgaat ttctgggtac
     2941 ggctacgggc accctggtag gcaagcaatg ttacatctct ccggaacaat ttaagggcaa
     3001 ggcctgcacc gcttccgatc tctactccct gggagccaca ctctactttc tcatcaccgg
     3061 tgaagacccc acacccatca gcagttcctc cccggcagag aaacgaccgg agataagcgc
     3121 cggcaccgac gccctggtga aagatctgac cgccctggag atagaagatc gcatcaagac
     3181 cgtggaggaa ttgcaggcgc gcctcacccc cttgctttcg accggggtgg gaaacctacc
     3241 atagtgatgc tgtgaaccgt cgttgccctt gagacggatc tgtctatact gacaatcacc
     3301 ccccaagtaa attcaaacag ataaagaggt taaggcatac catgacctta gaaaccaggc
     3361 ccgggccgca acgccagggg tcgcaattga accgcggcaa cgggctggag tccgcaaccg
     3421 gacaggacac cggcgatgca ggcaggctta atctcttaac ggcctcgcaa gagcaagaac
     3481 cggtctcaaa acaagtcaac aagctggcgc agtccggcgc caatctaaca agcggcggct
     3541 atttcgtcag tcgtctggct gaacaagaga cagccctggc caagcaaact gccgccgatc
     3601 cggtgaaggc tttgagcccc gccaccagaa aggagtttct ggcagccaga accgacctgg
     3661 aagccaaact cggtccggcg gtgggaagag tgaaagagca actgacacag ctcaaactgg
     3721 cacatcccgg cactatttac gaataccagg caccggctat cacagggcct cgcagtcgcg
     3781 acgagaaata cttccgggtc aaacccggcg ccaccttacc ggcccccatt caggaggcca
     3841 tgaacacaaa agaagcattg aaccaggcgc gcttgaccct ggctcgcacc gccggcaatg
     3901 gactgggcat aagcaagtcg ccccagcagc tggaagcagc catgcatctg aacaatccct
     3961 atcttgtcag aagcgggctg gcgaacgaag ccggccgtat ggaactggcg gcaatgaaag
     4021 tgcagaggga attgcacagc gaacttggta aggccggtca gaacgtgtca cgaaaagtgc
     4081 ttaccgccac tgccgctttc ggcacggcct ttgccgccaa caagctggta gataaagctc
     4141 tcttccacga ctcctccccc ggtttcttca cttacgcagc cgacatcgcc tctcccgctg
     4201 tgcttttgag cagacgcagt ctggccttca aggtgggtgt catgaccctg agccatgccc
     4261 tcggtaaagg caccgacctg gcctcaatca ggcgcacatc ctcaaagggc tagagagcca
     4321 gttgactctt gtctatggaa gaggtgctga aacctctttg ctcatcaaag gtgatgcgaa
     4381 tactgccgct ttcattggtc cagaaaaaga tgccggcgcc ggcattcacc ct
//
LOCUS       DAOQNZ010000126         4427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8663_length_4427_cov_3.774474, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000126 DAOQNZ010000000
VERSION     DAOQNZ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8663_length_4427_cov_3.774474"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKW73_03355"
     CDS             complement(<1..310)
                     /locus_tag="PKW73_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72346.1"
                     /translation="MTINDLSADLNLDQDRDQERIRRWRLVLGAAAESRLSYGMTSDD
                     AAMDGALSMLYDQEEEGEEKGKPNKRSAGLGASSPRVARWLGDIRKYFPSTVVSVMQK
                     D"
     gene            complement(297..2678)
                     /locus_tag="PKW73_03360"
     CDS             complement(297..2678)
                     /locus_tag="PKW73_03360"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNA72347.1"
                     /translation="MTTTIASKNKTDSAQVHLLGIRHHGPGSARSLEASLQEIKPDIV
                     LIEGPPEAEALIKYVQPSSKEEQADGEEAGGPGLIPPVALLIYAKEMPKLACYYPFAE
                     FSPEWRAMLYANQSHIPCRFIDLPQSHWLSMQSKQKQSANIDQVLSNLTEADKIRLDP
                     IGTLASLAGYEEGEVWWEQLVEQRSDSQIFEAVSEAMTALRTAVREDEEEERSKIDED
                     KQLFDEDYYIEPLREAHMRTCIRQAQKEGYKSIAVVCGAWHGPALAQAVKAKDDNQLL
                     KENLKDLPKLKVEATWIPWTNSRLSFESGYGAGITSPGWYEHLFHSDKGSDTTLSWLV
                     KAVKLLRAEDFDASSAQVIDAVRLVESLASMRNLHHAGLAELQQALVTCVLSGNEMPL
                     ALIEKKLLVGEKLGHVPAEVPGTPLMVDLQKTSTRLKLKQEALEKLLELDLRKPLDLE
                     RSSFLSCLQILGIAWGEKQKKKIKTSTFHETWKLQWQPSLSLAAVEASIWGKDVRQAA
                     TTRAIDLGTNSPTFAHLVDVLSLSLDGGLLEAVEKTMTLVGERSATCFDLGELMEALP
                     VLVGIARYGNVRGHNPQSMDTLITSITERIIVNLESSCLSLDEDAARRMQRQVTLMEE
                     ALRLFDKKELLEDSRAVLHRLASKSNIPALISGRSAKLLYECSYLSQEETVRLMHFAL
                     SAASAHSYSGQWLEGFLTNNAQVLIHDPIFFHIVDDWVKGIKTDAFQELLPLLRKTFG
                     AFSYSERRQISERVQSPGKTALAQTSERADDDRLLTEVLKTVQLMLGVNHDHQ"
     gene            complement(2740..3828)
                     /locus_tag="PKW73_03365"
     CDS             complement(2740..3828)
                     /locus_tag="PKW73_03365"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA72348.1"
                     /translation="MEATTDLLRQHAEAQFKEELSQLAAVDKRQKPPRWLLSPWAVLT
                     YLMGGKLENGYEVSPKYIGNKRIMEIAVATLTTDRALLLLGVPGTAKTWVAEHLSAAI
                     SGDSTMLVQGTAGTAEEAVRYGWNYARLIAEGPSMQALVPSPIMEAMKLGKIARLEEL
                     TRIPADVQDSLITILSEKTLPIPELGSEVQAVKGFNIIATANDRDKGVNELSSALKRR
                     FNTVVLPVPATLEEEVDIVARRTEALGRALELPAEKPALEEISRVVQIFRELRAGATL
                     DGKTKLRSPSGSMSTAEAISVMNSGLALAGHFGDGDLKAEDLAAGIVGAVVKDPVQDS
                     LVLKEYLETVLKEREGWKDFYRSLRHLI"
     gene            complement(3887..>4427)
                     /locus_tag="PKW73_03370"
     CDS             complement(3887..>4427)
                     /locus_tag="PKW73_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72349.1"
                     /translation="LLESLATFAATGEKEPQARELRRFQEELVNHASDKLTETSAGLR
                     FLKTLPADLAEALILSRLPRWAKPGSLGAPRLDLWNYLESTDFNWSKEFTARILDFIL
                     KESREKVPALSYTFTTNVKKFASQMHLSAAERAPDFYQVTEDSELNSYFVRPLLQIAE
                     TLNLRQDIEAAFKEKLPPH"
BASE COUNT          834 a         1191 c         1218 g         1184 t
ORIGIN      
        1 cgtctttttg catcaccgag accacggtgg aaggaaaata tttgcgaata tcgcccagcc
       61 agcgcgccac tctggggctc gatgccccca gtccggccga gcgcttattc ggtttgcctt
      121 tttcctcgcc ttcttcttcc tgatcataga gcatggaaag ggcaccatcc atggcggcat
      181 cgtcgctggt catgccgtag ctgaggcggc tttctgcggc agcgcccagg accagtcgcc
      241 agcgtcttat gcgctcttgg tccctatcct ggtcgaggtt caggtctgcg cttaggtcat
      301 tgatggtcat gattcactcc cagcatgagc tgaactgttt tgagaacttc agtcagtaac
      361 cgatcgtcgt ccgcgcgctc actggtttga gccagggctg tttttcctgg gctttgtacc
      421 cgctctgaaa tctgtcttcg ctccgagtaa ctgaaggctc cgaaggtctt gcgcaaaagg
      481 ggcagcagtt cttgaaaggc atccgtcttt atgcctttaa cccagtcgtc cactatgtgg
      541 aagaagatcg gatcgtggat caagacctgg gcgttatttg taagaaagcc ttccagccat
      601 tgaccgctat agctatgggc ggaagcggcg gaaagagcga agtgcattaa tctgactgtt
      661 tcttcctggc tcaggtagct gcattcgtat aaaagtttgg cgcttcttcc cgatatcagt
      721 gccggtatat tgctcttgct cgccagacga tgcaagacgg cacgactatc ttccagcagt
      781 tccttcttat cgaagaggcg caacgcttct tccatgagag taacttgtct ttgcatgcgc
      841 ctggctgcat cttcatcaag agaaagacag ctgctctcca gattgacgat tattcgttcc
      901 gtgatacttg tgatcagggt atccatagac tgcgggttgt gtccccgcac attgccgtaa
      961 cgggcaatgc ccaccaggac aggcagagct tccatcagtt cgcctaaatc gaagcaggtg
     1021 gcgctgcgct ctccgaccag ggtcatggtc ttctccactg cttccagaag cccgccgtca
     1081 agactgaggg aaagaacatc caccaggtga gcgaaagtgg ggctgtttgt gcctagatcg
     1141 atggctctgg tggtggccgc ttgcctgacg tccttgcccc agattgacgc ctccactgct
     1201 gccagactca gtgacggctg ccactgcaat ttccaggttt catggaaggt gctggttttg
     1261 attttcttct tttgcttttc gccccaggcg atgccgagaa tctgcaggca ggaaaggaaa
     1321 ctgctccttt ccagatccag cggtttgcgc aggtccagct ccagtagttt ttccagggct
     1381 tcttgcttga gcttgagcct tgtcgatgtc ttttgcaggt ccaccatgag tggcgtgcca
     1441 ggtacttcgg ccggcacatg ccccagtttt tccccaacca ggagtttctt ttcgatgagg
     1501 gcaagcggca tttcattgcc ggaaagaaca caggtgacca gggcttgctg caactcagcc
     1561 agcccggcat ggtgcaggtt tcgcatagac gccagacttt ccaccagccg cacggcgtcg
     1621 atcacttgcg cggaggaggc atcgaagtct tccgctctca gtagtttcac cgccttcacc
     1681 agccaggata aagtggtatc actgccttta tcgctgtgga agagatgttc gtaccaaccg
     1741 ggcgaggtaa tacctgctcc ataaccgctt tcgaaggaaa gacggctgtt tgtccagggg
     1801 atccaggtgg cttccacctt cagttttggt aagtctttca ggttctcttt cagtagctgg
     1861 ttgtcgtctt tggctttgac ggcttgagcc agtgctggcc cgtgccaggc gccgcagaca
     1921 acggcgatgc tcttgtagcc ttccttttga gcctgtctta tgcaggtgcg catgtgggct
     1981 tcgcgcaggg gctctatata atagtcttcg tcgaaaagtt gcttgtcttc atcaatcttg
     2041 cttctctctt cttcttcgtc ttctctgact gcggttctga gagccgtcat ggcttcgctt
     2101 actgcttcga atatctggct gtcgcttctc tgctccacca gttgttccca ccacacttca
     2161 ccttcttcat atccggccag tgaggccagg gtgcctatgg gatcgaggcg gattttgtct
     2221 gcttcggtga ggttgctcaa gacctggtcg atattggccg actgcttttg tttgctctgc
     2281 atggagagcc agtggctctg gggcagatcg ataaagcggc agggaatatg gctttgattg
     2341 gcataaagca tggcccgcca ttccggcgag aattcggcaa aagggtagta gcaagccagc
     2401 ttcggcattt ctttggcgta tatgagcagc gccaccggcg gtatcagtcc tggtccgccc
     2461 gcctcctcgc catccgcttg ctcttctttt gaagagggtt gcacgtactt gatcagggct
     2521 tccgcttccg gcggaccttc gattaaaact atgtccggct tgatttcctg aagggaggct
     2581 tccaggctgc gggctgagcc cggaccatga tgccttatgc caagaaggtg aacttgggcc
     2641 gagtcggtct tattcttgct ggcgatagtg gtggtcaatt tctaaacctg gctctccgct
     2701 tcctagtcat ttgccttcgt tattgtttca agcagtctct taaatcaaat gcctgaggga
     2761 gcgatagaaa tccttccagc cttcccgctc tttcagcact gtttcaagat attctttcag
     2821 caccaggctg tcctgcaccg gatctttcac tacggcaccg actatgccgg cggccaggtc
     2881 ttccgctttc aagtcgccgt cgccgaaatg gccggctaga gccagcccgc tattcatgac
     2941 tgaaattgct tcggcggtgc tcattgagcc ggaagggcta cgcaatttgg ttttgccgtc
     3001 gagggtggca ccggctctca gttctctgaa gatctgcacc acccggctga tttcttccag
     3061 ggccggtttc tcggccggca actccagggc ccgccccagg gcttccgtgc gccgagctac
     3121 aatatctact tcttcttcca gcgtggcggg taccggcaga accactgtat tgaagcgcct
     3181 cttgagggcg ctggaaagct cgttcactcc cttgtcccgg tcattagccg tggcgatgat
     3241 gttgaagcct tttacggcct gaacctcgga gcccagttcg ggaatgggca aggtcttttc
     3301 cgacaatatg gtgattagcg aatcttgcac atcagcggga attctggtca attcttccag
     3361 ccgggctatt ttgccgagct tcatggcttc cataatcgga ctcggcacga gagcctgcat
     3421 agagggccct tcggcaatca ggcgggcgta gttccaaccg tagcgcacgg cttcctcggc
     3481 tgtaccggcg gtgccctgca caagcatggt ggagtcaccg ctgatggcgg cagatagatg
     3541 ctccgccacc caggtcttgg ctgtacctgg tacacccaga agtaagaggg ctctgtccgt
     3601 ggtgagagtg gctacggcaa tttccataat gcgtttattg ccgatgtatt tggggctgac
     3661 ctcatagccg ttttccagct tgccgcccat caaataggtc agcactgccc atggtgataa
     3721 taaccagcgc ggcggtttct gccgtttatc cacggcggcc agttggctca attcctcttt
     3781 gaattgtgct tcggcatgct gtctcaggag atccgtggtc gcttccattc ttggctggtc
     3841 cttttggttt tctgtttgag ttttccgatt gagtttattg atgggtttag tgagggggaa
     3901 gtttttcttt gaaggcggct tcaatatcct ggcgtaaatt gagggtttcc gctatttgca
     3961 aaagagggcg gacgaagtaa gaattgagtt cgctgtcttc agtcacctgg tagaagtcag
     4021 gggctctctc ggcggccgaa aggtgcatct gtgaggcgaa ttttttcaca ttggtggtga
     4081 aggtgtagct cagggccgga accttctcac gactctcctt cagtatgaag tctagtattc
     4141 gggcggtgaa ttccttgctc cagttgaagt cggttgactc caggtaattc cagagatcta
     4201 gtctgggtgc cccgagacta ccgggcttgg cccagcgggg cagccgactt aagataagag
     4261 cctcggccag atctgccggt agggttttaa gaaaacgtag accggcactg gtttccgtca
     4321 atttgtcgga agcgtggttg accagttctt cctggaagcg acgaagttcc cgagcctgcg
     4381 gttccttttc gccagtggcg gcaaaagtag cgagggattc cagcaag
//
LOCUS       DAOQNZ010000127         4426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8669_length_4426_cov_3.638069, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000127 DAOQNZ010000000
VERSION     DAOQNZ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8669_length_4426_cov_3.638069"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..451)
                     /locus_tag="PKW73_03375"
     CDS             complement(80..451)
                     /locus_tag="PKW73_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72350.1"
                     /translation="MGRDIDDEKPVQKIEQAQPQAEKHEPLNLLDLTQAGADQSLAKN
                     SDKSREIPNAVQEHVAGLSENMNKYMEKLQGATPEDMAYYKQGLKDTLAYMSDPESKW
                     CGGGAYSKDQVQQLSKSLDKS"
     gene            complement(714..2093)
                     /locus_tag="PKW73_03380"
     CDS             complement(714..2093)
                     /locus_tag="PKW73_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNA72351.1"
                     /translation="MLLALVLAPFWLAGTSAPTLAAGPSPIKRAREVGAGQIARARAA
                     LSEKPPFVIRDKWAVLVGVSRYNDPSINGSASAARNVLTLTKTLIDPECGRFAPDHVL
                     VVTDGHATKESLARATYQDWLIKKALPSHLILIYLALKLTASDDGTDLLLLPADASLK
                     DKELTSVSLSGMLQEIKRRTQSKNIVLLLDATTQDGKSAAAQLATIARQTGITLLSAD
                     AGLAASANDDFSKNSLFALYLSEGIKTGAGQMPIESIAGYVSEVIAKAPPAQKLLQKP
                     LYLAGEEPELVKQPLGLKIKLPFDPKNVKVGHPLDTLAEKRPDLAAGLSANFKAPLED
                     KRKPQTLQELDRKKEEAEAAADNDDDDDAGAEQVDFEGYMKDMKKSIQAKWQPPKGIA
                     QKTIVAVFSIQRNGNIVEADIVESSGDAGIDQAAMKALKDASPLAPLPKGAPAHVQVR
                     YKFDWKVSQ"
     gene            2740..3603
                     /gene="rsmI"
                     /locus_tag="PKW73_03385"
     CDS             2740..3603
                     /gene="rsmI"
                     /locus_tag="PKW73_03385"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNA72352.1"
                     /translation="MSGKLFIIGTPIGNLEDISQRVINALRGCSLIFVEDTRVSVKLL
                     NHLGLSVPMLSCHRHNEHERLRALEKAASDNASVGLLSDAGMPLISDPGEPLIQKAIE
                     LNMEIIPIAGPTAFVLALVASGFDLSSFAFEGFLPDKESLIIEKLARLKGETRTLVFY
                     VSPHKLATTLKLMLQVLGDRRACLARELTKFYEEFVRLPLSELSAKYEELEPRGELVL
                     VLEGASASSTDYSDWQAVESQRQEVLEHVKRNLAHGLKLSKAAALAAEYFSIGKSDIY
                     RASIAELKDSV"
     gene            3674..>4426
                     /locus_tag="PKW73_03390"
     CDS             3674..>4426
                     /locus_tag="PKW73_03390"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNA72353.1"
                     /translation="MEEHINAAAGSISLVEAIVLGIVQGLTEFLPISSTAHLRVVPSL
                     LHWQDPGVAYSAVIQLGSLIAVLAYFYKDIFQIAFGSLKALKEKDFGSRDLRLAGAII
                     IGTMPVCVVGLLLKHLLEQNDGPFRSLTVIGCAAIFMGLALIAAEKLGKHTRVLDQMG
                     VRDGLLVGLGQAMALIPGCSRSGSTLTVAMLIGLKRADAARFSFLLGVPAIFLSGMLE
                     LKSMFETGLSGEGIGSLVAGLLSSTIVSYLAIA"
BASE COUNT          911 a         1077 c         1228 g         1210 t
ORIGIN      
        1 ttttcaagag cttaataaac tctggttaaa gaaagagagc cgccagggcg gctctctctt
       61 tgaaatgctg ttgcagtctt tagctcttat cgagggactt gctcagttgc tgtacctggt
      121 ctttgctgta ggcaccgcca ccgcaccatt tgctttcggg atcgctcatg taggccaggg
      181 tgtccttcag cccttgcttg tagtaagcca tatcttcggg ggtggcgccc tggagttttt
      241 ccatgtactt gttcatgttt tcgctgagcc ctgccacatg ctcttgcacg gcattgggaa
      301 tctcacgcga tttgtcggag ttcttggcaa ggctctggtc ggcgccggcc tgagtcaggt
      361 cgagtaagtt gaggggctcg tgcttttcag cctgtggttg agcctgctct atcttttgaa
      421 ctggtttttc gtcgtcaata tcacgtccca tgatttgaat ccttcgcctt atagaagtag
      481 aagaccagtg gtcttgtttt ggggattgct tttaaattgt tagtaacagg ttttggttag
      541 gtgcagttca cctgttattg agattacgct tcgttaatgc gatcgtcaat gtggtttcta
      601 cgtaggcgcc cggagataag cctggaaacc ctgaatttac gggagtcggc gtggcgtatt
      661 ttatctgccg cgggagggat gtgtcagact gcggcacttg acaacccggg ctgttactga
      721 ctcactttcc agtcgaactt gtagcgcacc tgcacatggg cgggggcgcc tttgggcagt
      781 ggagcgaggg gggaggcgtc cttgagggct ttcatggcgg cctggtcgat gcctgcgtcc
      841 cccgagctct ctacaatgtc ggcttccaca atattgccgt tcctttgtat agagaaaacg
      901 gccactattg tcttttgagc gatacctttg ggtggctgcc acttggcttg tatggacttc
      961 ttcatatctt tcatatagcc ttcgaagtcc acctgctcgg ccccggcgtc atcgtcatcg
     1021 tcgttgtcgg ctgcggcctc ggcttcttcc ttcttgcggt ccagttcctg aagggtctga
     1081 ggttttctct tatcttccag gggggctttg aagttcgcgc tcagccccgc tgccaggtct
     1141 ggtcttttct cggccagggt atcgagggga tgccccactt tgacgttttt gggatcaaag
     1201 ggaagcttga ttttgagacc gaggggctgc ttgaccaact ccggttcttc cccggccagg
     1261 taaagcggtt tctgtaagag cttctgggcc ggcggcgctt tcgctatcac ttccgagaca
     1321 tagccggcaa tgctttcgat gggcatctga ccggcaccgg ttttgatgcc ttcgctcaaa
     1381 taaagggcga agagcgagtt tttactgaag tcatcgttgg cggacgctgc cagaccggcg
     1441 tcggcgctaa gcaaagtgat gccggtttgc ctggcaatgg tggctagctg ggcagcggca
     1501 gacttgccgt cctgggtggt ggcatctaga agcagcacta tgtttttgct ctgggtgcgg
     1561 cgtttaattt cttgcagcat gccgctcagt gaaaccgagg tcagctcttt atctttcaaa
     1621 ctagcatcgg caggcaaaag caacagatca gtgccgtcat cgctggcagt tagtttgaga
     1681 gctaaataga tgagaatcag atgggagggc agagcttttt tgattagcca gtcctgataa
     1741 gtggcgcggg ccaggctctc cttggtggca tgaccgtccg taactacaag tacgtgatcc
     1801 ggggcgaagc gtccgcattc gggatctatc aaagtcttgg tgagggtgag cacattcctg
     1861 gccgcagagg cactgccatt tatagatgga tcgttgtagc gagatactcc caccaaaacg
     1921 gcccacttgt cgcggattac aaagggtggt ttctccgaca gtgcggcccg ggctctagct
     1981 atctgaccgg caccgacttc ccgcgcgcgt tttatggggc ttggaccggc ggcaagagtg
     2041 ggggcgcttg tgcctgctaa ccagaagggg gccagcacca gggcgagaag catggccctc
     2101 acttgatttt gcggcaggga atggcgtggt ggaacttgaa tggttgcact tcctatttat
     2161 cagaagtcga cttgccgact gattgcagct gtctatagtt ggagcctact gacctcaaaa
     2221 agacaagcca tccagatgat atacagtctc aagcagcgtt gccatacttg ccttcttata
     2281 tgtccggaag gccttgcact gccccggcct ttcgtatata cgcttatgcc tgtttcttat
     2341 taatcgggat tttgtctgtt acttgttttt gagccaattg ggctgccaaa aacttttcca
     2401 cgatattgaa aactatggaa agccgcttag agagccagag ttgtacctgc gaattggtgt
     2461 tatccgcacc tggagccgtt tttaatcata tgagaggcgt ctcgaaattt gtaaaggctc
     2521 ttttaatcga tgggagcgac aatccttttc gcaagcaagg ttttaagctt aagttagcca
     2581 attaaattac acacaggtgt agaaaacctg tagaaaactc ttcttgtcag gggggaactc
     2641 cttaagccga ttgaaagata aaatgctcta acgtgccaga tgtgttgcgt ttcagccttg
     2701 gcaaggtttt ctacagaaag gctaagggga gttatcaagt tgtcgggaaa gttgttcata
     2761 atcggcacgc cgataggaaa tctggaagat atatctcaga gggtaattaa tgctctaaga
     2821 ggttgcagcc tgatatttgt tgaagatacg agagtaagcg tcaaactgct caaccacctg
     2881 ggcttgagcg tgcccatgtt gagttgccac agacacaatg agcatgagcg cttacgggcc
     2941 cttgaaaagg ctgcatccga caatgcctcg gtggggcttt taagtgatgc cggtatgccc
     3001 ttgattagtg atccagggga gcctctcatt caaaaggcta tcgagcttaa tatggagata
     3061 atccccatcg ctggtcccac ggcctttgtc ctggcgcttg tggcgagcgg cttcgacctt
     3121 tcctcttttg cctttgaggg ctttctgccc gacaaagaga gccttattat tgagaaactg
     3181 gccagattga aaggcgaaac gcgcaccctg gtcttctatg tatctcccca caaactggca
     3241 acgaccttga agctcatgtt gcaggtactg ggagaccggc gcgcctgtct ggctagagaa
     3301 ctgaccaagt tctatgagga gttcgtgcgc ctgccgcttt cggagctttc tgccaaatat
     3361 gaagagcttg agccccgcgg cgagttggtg ctcgtgcttg aaggggcatc tgcttccagc
     3421 accgactaca gcgactggca ggccgtagag agtcagaggc aggaagtttt agaacatgtg
     3481 aagcgaaacc tggcccacgg gcttaaattg tcaaaagcgg cagcattagc cgctgaatac
     3541 ttttctatcg gtaaatcgga tatctaccgg gcctctattg ccgagctgaa agatagtgtc
     3601 tgagccgttt cctctctctt atgaggttga ctgccgggtg ctaacataag tcagtccctc
     3661 tctggtagaa gctttggaag aacatataaa tgccgccgcc ggctccatat ctctggtaga
     3721 agccatagtg cttggtatag tgcagggatt gaccgagttt ttgcccataa gctccaccgc
     3781 ccatttgcgc gtggtgcctt ctctcttgca ctggcaagat cccggtgtgg cttacagcgc
     3841 cgtcattcag cttggttctt tgattgctgt tctggcctac ttctacaaag atatctttca
     3901 aattgccttc ggctctctta aggcgttgaa agaaaaggat tttggcagcc gcgatttgcg
     3961 tctggccgga gccattatca tcggcaccat gccggtttgt gttgtggggc tgctgctcaa
     4021 gcatctgctc gaacaaaatg acggaccttt tcgctctctt acggtaatag gctgcgctgc
     4081 catttttatg ggactggctt tgatcgccgc cgaaaaactg ggtaagcata cgcgcgtgct
     4141 ggatcaaatg ggcgtcagag acggacttct agtagggctt ggccaggcta tggctcttat
     4201 ccccggttgc tcccgcagcg gttcaaccct tactgtggcc atgctgatag gcttgaaacg
     4261 agctgatgct gccaggttta gcttcctgct cggggtgccg gcaatttttc tctccggcat
     4321 gctggagttg aaaagcatgt ttgaaaccgg cttgagcggt gaaggcatag gcagcctggt
     4381 ggccggactt ctatcttcca ccattgtcag ttatctggcc attgcc
//
LOCUS       DAOQNZ010000128         4425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8673_length_4425_cov_3.965217, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000128 DAOQNZ010000000
VERSION     DAOQNZ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8673_length_4425_cov_3.965217"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKW73_03395"
     CDS             <1..494
                     /locus_tag="PKW73_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNA72354.1"
                     /translation="PEATEALSQYLQEPVRLVRMSRTFTRQVDENYATAADTVGFADG
                     FPMLVISDASLSDLNGKLEYPVEMSRFRPNLTVSGATPFAEDTWKKLQLDSVRMELVK
                     PCSRCQITTIDQDSADTGKEPLRTLSGYRKIDGKVMFGQNALVRYEGDFASIRLGETI
                     TVA"
     gene            complement(520..2349)
                     /locus_tag="PKW73_03400"
     CDS             complement(520..2349)
                     /locus_tag="PKW73_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72355.1"
                     /translation="MAPRAKSKNVPNPAEAALYAGWQVLKKHPLFGPLADATSLRNSK
                     GSDNFCPQDGYVVVCSYGVLHCHPTRRASVENWTYVLAHALLHLGFGHLKEVSREREW
                     NAACCAFVTRFLKDLKLGQPPEFACGHFEITAQNEEALYRLFCEQGIAPDLSGLGTAG
                     AHNDFLGVSSAPQYYNCQYGSFGTRTYEELLADGLMAAVTSAVNVAGGLEPYLGAGEK
                     LHTAMQQARSWFISSYPLLGALAAAFKIVEDVAVCQRLNISIAAVDAEAKEIYCNPNA
                     KMNEAQARFVIAHELLHVGLQHDARQQGRDPYLWNVACDYVINGWLVEMGIGEFPHFG
                     GLHDPALKGQSAEEIYLTICKDLRKYRKLATFRGIGCGDMLRGDLGEWWRRPQGMALD
                     EFYRSCLAQGLTFHHQQCRGLLPAGLVEEINALSQPPIAWDVELAQWFDLYFAPLHKK
                     RSFARISRRQASSPDIPRPRYVPEEALLEGRTFAVVLDTSGSMDRKILAKALGAIASY
                     CMSRDVPAVRLVFCDAYAYDEGYVAAETIAQRVRVRGRGGTVLQPGIDLLESLEDFPK
                     KGPILIITDGFCDNLSVKREHAYLLPQGRYLPFHPKGPVFRIT"
     gene            complement(2327..3433)
                     /locus_tag="PKW73_03405"
     CDS             complement(2327..3433)
                     /locus_tag="PKW73_03405"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNA72356.1"
                     /translation="MHQGKDGEFSLNPVVTVTQTELFDFLLNVAVCRPVFIWGPPGIG
                     KSSIVQQFAKSVGLPCVSLLGSQLAPEDIIGVPQIIDSVSRFCPPAMIARSEPYCLFL
                     DELNACSHEVQKAFYSLIHERRIGEYELPVGSIVIGAGNRSQDSAIVKPMSSALMNRL
                     VHVHLRVSHRDWLAWALENNIHSLVVEYIQTRTDQLWTQPPKHEEPFSTPRSWHMLSD
                     ALHEYGDKLDDKVIELLAYGCLSPQHATQFKAFNKQVKNRFKLSSIMSGDMKWPAEAE
                     DRDLLYFLAQSFRSQLLRDLPAEKDAVTGRHRELAHTGKALLKDLSVISLEIAQMVVA
                     EDENGKTLPSWFLVEVVRDLPRLVDRKDGAQSKV"
     gene            complement(3523..>4425)
                     /locus_tag="PKW73_03410"
     CDS             complement(3523..>4425)
                     /locus_tag="PKW73_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA72357.1"
                     /translation="LLAYWSNYLIGLMPLGLTEWRWQLGVSGIPALLFLVMLYGIPRS
                     PRWLVAREQVEEARQVLLKTSMGDAEAELAAIVADVSEEKANKEERLFTRRHRFPIFL
                     AIATGAFNQLTGINAILYYLNDIFALAGYNKISGGMQAVVIGATNLIFTLIAMAVIDK
                     LGRKQLLLIGSVGMCLCLLGVAVTMQNHALMQSLVWYLIAYIGFFAFSQGAVIWVYIS
                     EVFPTMVRSRGQSLGCSTHWIMNALISGTFPMVAEASGSLPFYFFSAMMLVQFLVVFF
                     IFPETKGISLEQMEKQLAGGGKGH"
BASE COUNT         1153 a         1238 c         1124 g          910 t
ORIGIN      
        1 aaccggaagc cacggaagct ctctcccagt atctgcaaga accggtgagg ctggtgcgca
       61 tgtcccgcac atttacgcgg caggtagacg aaaactacgc cacagccgct gataccgtcg
      121 gatttgccga cggcttcccc atgcttgtca taagcgacgc atccctctca gatttgaatg
      181 gtaaattgga atatccggtg gaaatgagcc gcttccgccc taacctcacc gtatctggtg
      241 ccacgccctt tgccgaagat acctggaaga aactacaact tgactcggtc aggatggaac
      301 tggtcaaacc ctgctcccgc tgccagatta ccaccatcga tcaagacagc gccgacaccg
      361 gtaaagagcc gctgcgcact ctctccggct atcgcaaaat agacggtaaa gtcatgtttg
      421 gacaaaatgc cctggttcgg tacgaaggag actttgccag tatccggctg ggcgagacca
      481 tcaccgtggc ttgaattgaa cggtgggcaa tgagcctacc taggtaattc tgaagaccgg
      541 cccctttgga tgaaacggca ggtagcgccc ctggggcaaa agataggcgt gctcacgctt
      601 gactgaaagg ttgtcgcaaa aaccatcggt gataatcaat atgggtccct tcttcgggaa
      661 gtcttccaag ctttccaaca aatcgatacc aggctgtagt acagtaccgc cccggccacg
      721 caccctcacc cgctgggcaa tggtctcagc cgccacatag ccctcgtcat aagcataggc
      781 atcacaaaag acgaggcgca cggccggtac atcgcgagac atacagtagc tggcaattgc
      841 ccccagggct ttggccagga ttttgcgatc catggaaccg gaagtatcaa gcacaactgc
      901 aaaggtacgc ccctccagaa gggcttcctc cggcacatag cggggtcggg gaatatccgg
      961 gctactggcc tggcgccgac ttatacgggc aaaacttcgc ttcttgtgca gaggcgcaaa
     1021 atagagatca aaccactgag ccagctcaac atcccaggca ataggcggtt ggctcaaagc
     1081 atttatctct tccaccagcc ctgccggcaa gagaccccgg cactgctggt ggtgaaaagt
     1141 caagccctgc gccaggcaac tgcgatagaa ctcatccagg gccatgccct ggggacgccg
     1201 ccaccactca cccaggtcgc cgcgcagcat gtcaccacaa ccgataccac gaaaagtggc
     1261 cagcttacga tacttccgca agtctttgca tatggtcaaa tatatttctt ccgccgactg
     1321 ccccttcaaa gccgggtcgt ggagcccacc gaagtgaggg aattcgccaa tacccatttc
     1381 taccagccag ccgttgatga cgtaatcaca ggcgacattc cacaaataag ggtcgcgccc
     1441 ttgctgcctg gcgtcgtgct gcagtcccac atgcaataac tcgtgagcga tgacaaagcg
     1501 agcctgtgct tcgttcattt tggcattggg attgcaataa atttccttag cttcggcatc
     1561 tacggcagcg attgaaatat tcaaccgctg acaaacggca acgtcttcca cgatcttaaa
     1621 agcagccgcc agagccccta gcagaggata ggaactgatg aaccaggagc gggcctgctg
     1681 catggccgta tgcagttttt caccggcacc aagataaggc tccagcccac ccgctacatt
     1741 gacggcactg gtcactgccg ccatgagacc atcggccaga agctcttcat aagttcttgt
     1801 gccgaagcta ccgtactggc aattgtaata ttgcggcgcg gaagagacac ccaggaaatc
     1861 gttgtgtgcc cccgccgtgc caagcccgct cagatcaggc gctataccct gctcgcaaaa
     1921 cagtctatat agagcctcct cgttctgagc ggtaatctca aaatgtccgc aggcaaactc
     1981 cggcggctga cccagtttca agtctttcaa aaaccgcgtg acaaaggcac agcaagcagc
     2041 attccattct ctctctctcg atacttcttt gagatggcca aaacccagat gcaaaagggc
     2101 atgagccaat acataagtcc aattctccac actggcccgc cgggtgggat ggcagtgcag
     2161 aacaccatag gagcagacaa ctacgtatcc gtcctgggga caaaagttat cgctgccttt
     2221 actgtttcta agcgaagtag catcggccag cggaccaaac agggggtgct tcttgagcac
     2281 ctgccagccg gcatagagtg ctgcttccgc cggattggga acgtttttag actttgctct
     2341 gggcgccatc ttttctatcc accaggcggg gcaggtctct tactacttcc accaggaacc
     2401 aggaaggtaa ggttttaccg ttctcgtcct cggcaacaac catctgggca atttccagac
     2461 tgataacgga taaatctttt aaaagcgcct tgcctgtatg cgccagttct ctatgacggc
     2521 ctgtgaccgc atctttttcc gcaggcagat cacgtaagag ctgagaccga aaggactgag
     2581 ccagaaagta aagcaaatct ctatcttccg cctccgccgg ccacttcata tcgccggaca
     2641 taatggaact taatttgaag cgattcttca cttgcttgtt gaaagcttta aactgcgtgg
     2701 catgctgcgg cgaaaggcaa ccgtaagcaa gaagctcaat taccttatca tcgagtttat
     2761 caccgtattc atgtaaggcg tcgctgagca tgtgccagga acgcggcgta gaaaagggct
     2821 cctcatgctt gggcggctgc gtccagagct gatcggtgcg ggtctgaata tactccacca
     2881 ccagagagtg gatgttgttc tccagagccc aggccaacca atctcggtga gaaacccgca
     2941 agtgcacatg caccaggcga ttcatcaagg cggaagacat gggtttaact atggcgctat
     3001 cttgagagcg attgccggca ccgatgacta ttgagccaac aggcagctca tactcaccga
     3061 tacgcctttc atgaatcaaa ctgtaaaagg ccttttgcac ttcatgggaa caggcattca
     3121 gttcatcaag gaagagacaa tagggttcgc ttcgggcgat catagctggc gggcaaaagc
     3181 gactgaccga atctataatc tggggcacgc cgataatgtc ttccggcgca agctgactgc
     3241 ctaaaagaga aacacaggga agccccaccg acttggcgaa ttgctgcaca atggaagact
     3301 taccgatgcc gggcggtccc cagatgaaaa ccggacgaca aacggcgaca ttcaaaagaa
     3361 agtcgaaaag ctcggtctgt gtgactgtta ctaccggatt caaggaaaac tccccgtctt
     3421 taccctggtg catactggtt ccaactttat accctcacat aaagtcgata cagcaattgt
     3481 aaattgggcg gttaagccga gggtcaggtc aaaaacgcct gctcaatgcc ctttgccgcc
     3541 cccggccagt tgtttttcca tctgctcgag actgatgccc ttggtctcgg ggaaaatgaa
     3601 aaagaccacc agaaactgca ccaacatcat cgcagagaag aagtaaaaag gtagcgaacc
     3661 tgaggcttcc gccaccatgg ggaaggtgcc tgagataagg gcattcatga tccagtgcgt
     3721 ggaacagccc aggctctgac cacgggagcg caccatggtg ggaaagactt cgctgatata
     3781 gacccagatc actgccccct gggaaaaggc aaaaaatccg atgtaggcta ttaagtacca
     3841 gacaagagac tgcatgagag cgtgattttg catagtcacg gcaacgccga gaagacagag
     3901 gcacataccc accgaaccta tgagcaggag ttgctttcga cctagcttat cgattacggc
     3961 catggcaatc agagtaaaga tcaaattggt ggcaccgatg accacggcct gcatgccgcc
     4021 gctaatcttg ttgtaaccgg ccagggcgaa aatatcattt aagtaataca aaatagcatt
     4081 gatgcccgtg agctgattga aggcaccggt ggcgatggca agaaaaatag ggaagcgatg
     4141 gcgcctggtg aaaagccgct cttccttgtt ggccttctcc tcggaaacat cggccacaat
     4201 ggccgccagt tcggcttcgg cgtcacccat actggtcttg agcaatacct gccgggcttc
     4261 ctctacctgc tctctggcta ccaaccagcg cggactgcgg ggtataccgt agagcatgac
     4321 cagaaagagt agggcgggaa taccggaaac gcccagttgc cagcgccatt ctgtcaaacc
     4381 aaggggcata agaccaatca ggtaattgct ccagtaagca agcaa
//
LOCUS       DAOQNZ010000129         4409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8711_length_4409_cov_3.361966, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000129 DAOQNZ010000000
VERSION     DAOQNZ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8711_length_4409_cov_3.361966"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKW73_03415"
     CDS             complement(<1..414)
                     /locus_tag="PKW73_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72358.1"
                     /translation="MGSPLQKPAAKLNSACLKGIILASALTLSMQAGQPAQSESTSGS
                     DTWSSCLSRGQEALTAQDLTLAEESFRKALALVESKPHKPADEEQCLRKLADALMLRD
                     KTAEAQALYQRLLNNLTSRYGEKSNKLGPVLLALGS"
     gene            complement(536..1864)
                     /locus_tag="PKW73_03420"
     CDS             complement(536..1864)
                     /locus_tag="PKW73_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72359.1"
                     /translation="MTQEKDKVSEAPKVAENNQLASVDRLELLSSPQPPRSDAGGRDS
                     LPPLTIDGTDSGHKPDKKSEPFELISTSKDMDPSKPTAVFLDQFARKDVDLGGTKVAH
                     GEISRAAAEENGFNTVALNLQAPESLGNGTDYSKAINKIADMAEDGKLPLGKGDVLNI
                     SMGNLDPTFEEASKFLGFPVTAQNLASQRENILKRMQEISQDESRSKEDRETAERVVN
                     TNSAIDRLQDKGVEVVHSAGNDGQDRFSWDFMNAKWQLSSNRPSGKPDEFSASHSLTT
                     SADGVLPLKIKQEGDLISPVPMAEQKGKVAIADTGVEFPIDSKSPFSGNSRIFNRENI
                     NPKFSQPMATPQEKAVPEQLFIPELTFSSQEKLPWTDKSSAELLSFTGKSELPKRMEM
                     AYPMSDVIKKITSNPGPGEELMSGVINGTSFSNIGFLKDQRARLEALKQN"
     gene            complement(2106..2732)
                     /locus_tag="PKW73_03425"
     CDS             complement(2106..2732)
                     /locus_tag="PKW73_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase domain-containing protein"
                     /protein_id="HNA72360.1"
                     /translation="MKVVFLSDTHNSLSKLKVPDGDLLIHAGDFCSAGRLDEVARFND
                     DIMKLPHKHKVVVAGNHDWPFQRQPKEARKLLHESIIYLEDAAAVVEGLHIYGSPWQP
                     AFFNWAFNLPRLSVALKERWQAIPDNTDILVTHSPPHGIMDRVPRGEMVGCEHLRRRV
                     DRIQPRIHCFGHIHCGYGQETRGKTLFINASNLDEDYLPANEPITVEL"
     gene            complement(2745..4148)
                     /locus_tag="PKW73_03430"
     CDS             complement(2745..4148)
                     /locus_tag="PKW73_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72361.1"
                     /translation="MPGSQAKQFSSEPGRQQTSDTGRRAKPLLLWLPLVFSLNLATTA
                     DCTAQPPQTEPVKVRCALPAGKNPKVLALFKQAGTLLNEKKFQAAIDVYSYAISIEPK
                     NGYAYFHRAACYGSLGKRADSLKDLKVYAHLLATRPGATDNDKLLELYLPQVASTLNR
                     YENSEKGRAALTKDLNNVMGKSNININKPHIGLQAPSPEDLPAYRKCKPLFDKLEHLK
                     VTGDDTGALGVAREIVRLLPNYSYCHQQLGCCYFDLDKLPLAIEEFNKGVALNPKDPY
                     VLMSRAIAYSLDGQYERAEKDYDGALALASYRTIILDDLMAVQSGAFAVPSKDEIWLR
                     KGELAMKQKQNAKALKAFDEAIKFNKRSDGAFLLRARCHESMGNLDAAIADYNAILQF
                     NPKDEAAYKARGDLYFKKKDYPKALSDFSRVIALEPREAWRAYDARAKVFDKLGNAAA
                     ARADRQMVSKLGFQPSR"
     gene            complement(4166..>4409)
                     /locus_tag="PKW73_03435"
     CDS             complement(4166..>4409)
                     /locus_tag="PKW73_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72362.1"
                     /translation="AMTIVTLAPIFLAGLIFATAFKGAEKPSRSFAFNLLGSVLGGLL
                     EYLSTYLGISNLLLVALLLYVFSFCYMLKVKPETAS"
BASE COUNT          824 a         1074 c         1323 g         1188 t
ORIGIN      
        1 ggagcccagg gcaagcagta ccggtcccag tttgttggac ttttcaccgt agcgactggt
       61 caaattgttc aagaggcgct gatagagcgc ctgggcttcg gcggttttat ctctgagcat
      121 gagggcgtcg gctaatttgc gcaggcattg ctcttcgtcg gccggcttgt gaggcttgct
      181 ttcgaccagt gccagcgctt tgcgaaagct ttcttccgcc agggtcaggt cctgcgccgt
      241 cagggcttct tgccctcgcg aaaggcaact ggaccaggtg tcgctgccgc ttgtgctctc
      301 actttgagcc ggctgaccag cctgcatgga aagggtgagg gctgatgcca ggataattcc
      361 cttcaggcat gccgagttta gttttgccgc gggcttttgc agagggcttc ccataaaatt
      421 gacaacctca agtatctggc gcttgcacct atagtttaac cggtgcagag agactgaagc
      481 ttggaaaaga gctgcaaggg cggttgtcag caaagcccgc cgtggcaggt tgcatttagt
      541 tttgcttgag ggcttccagc ctggctcttt ggtctttcaa aaagccgata ttggagaagg
      601 aggtgccgtt gatgacgccg ctcatgagct cttcgccggg accagggtta ctggtgattt
      661 ttttgattac atcgctcatg ggataagcca tttccatacg tttgggaagc tcgctcttgc
      721 ctgtgaagga gagcaactct gcgctggatt tgtcggtcca gggcagtttc tcctggctgg
      781 agaaggtcag ttcgggaatg aagagttgtt cggggactgc cttttcttgc ggtgtagcca
      841 tgggctggct gaatttggga ttgatatttt cccgattgaa aatgcggctg ttgccggaga
      901 atggggattt gctgtcgatg ggaaactcta cgccggtatc ggcaattgct actttgccct
      961 tttgctcggc catgggtacc gggctgatca gatccccttc ttgttttatc ttgaggggca
     1021 gcacaccgtc ggcactggtg gtgagggaat ggctggcact gaattcatca ggtttacccg
     1081 aggggcggtt ggaagagagt tgccacttgg cgttcataaa gtcccagcta aaacggtcct
     1141 ggccgtcgtt gccggccgag tgcactacct caacgccttt gtcctgcagg cggtcgatgg
     1201 cgctattggt gttgactaca cgctcggcgg tttccctgtc ttccttggag cggctctcgt
     1261 cctggcttat ctcctgcata cgcttcagta tgttttcgcg ctgggaagcg agattctggg
     1321 ctgtgacggg aaagcccagg aatttggatg cttcttcgaa ggtggggtcg agattgccca
     1381 tactgatgtt cagtacgtcg cccttgccca gaggcagttt gccgtcttcc gccatgtcgg
     1441 cgattttgtt gatggctttg gaatagtcgg tgccgttgcc cagagattcg ggagcctgga
     1501 gattgagcgc aaccgtgttg aagccgtttt cttccgccgc tgcccgactt atttcgccgt
     1561 gggccacctt ggtgccgccc aggtctacgt ctttcctggc gaattggtcc agaaagactg
     1621 cggtgggctt agaggggtcc atatctttgc ttgtgctgat cagctcaaag ggctcggatt
     1681 tcttgtccgg cttgtgcccc gaatccgtgc cgtcaatggt caggggcggc agtgagtctc
     1741 tgcctccggc atcacttcgc ggaggctgcg ggctcgacag caattcgagt cggtctaccg
     1801 aagctagctg attgttctca gctacttttg gggcctcgct gactttgtcc ttttcctgag
     1861 tcatgaaatg gggcgacctt tgccgaggca gtgcggcgct gtctagctct gggatggtaa
     1921 ggggtaagcc ttatataccc cggctcgccg gaatttaagc ccagtgcttg tttctagctc
     1981 tctgccaaag cgctgtcaat aaatgcgctc cggaaaagat ccaggtttta gcctcgttat
     2041 cttcctgagg ttgatgctcc gatttgccgc tgtaccctga tgactctagg gttatagctc
     2101 tactgttata gttctactgt tattggctca ttggcgggca ggtagtcttc atcaaggttg
     2161 gacgcgttaa tgaaaagagt cttgcccctc gtttcttgcc cgtagccgca gtgaatatgc
     2221 ccgaagcagt ggattctagg ctggatgcga tccacccgcc tgcgcaagtg ctcgcagcct
     2281 accatttctc cccgcggtac gcggtccatg ataccgtggg gcgggctgtg ggtgacgagg
     2341 atgtcggtat tgtccggaat ggcttgccag cgttctttca aagctacgga gaggcgcggc
     2401 agattgaagg cccagttgaa gaaggcgggc tgccatggac tgccgtagat gtgcagacct
     2461 tctaccacgg cggcggcatc ttccagatag atgatgcttt catggaggag tttgcgagcc
     2521 tctttgggtt gtctttgaaa gggccagtca tggttgccgg ccaccaccac cttgtgtttg
     2581 tggggcaatt tcatgatgtc gtcattgaag cgcgccactt catcaagacg accggcgcta
     2641 cagaagtcgc cggcatgaat gagcaggtcg ccgtccggca ccttcagttt ggaaaggcta
     2701 ttgtgggtgt cggagaggaa gacgactttc ataaagccat tttactatcg gctgggctga
     2761 aaacccagct tgcttaccat ttgtctatcg gcacgggcgg cggcggcgtt gcctagtttg
     2821 tcaaagactt tcgctctggc gtcgtaagcg cgccaggctt ccctgggttc cagggctatg
     2881 acccggctga agtcgctgag cgcttttggg tagtctttct tcttgaagta gagatcgcct
     2941 cttgctttgt aagcggcttc atcttttgga ttaaattgca aaatggcgtt atagtcggcg
     3001 atggccgcgt ctagattgcc catgctctca tggcagcgag ctcgtaagag aaaggcgcca
     3061 tccgagcgct tgttgaattt gatggcttcg tcaaaggctt tcagtgcttt cgcattctgt
     3121 ttctgtttca tggccagttc gcctttgcgc aaccagattt cgtctttgct gggcacggca
     3181 aaggctccag actgaacggc catgagatca tccagaataa tggtgcggta gcttgccagg
     3241 gccagggcac cgtcataatc tttctccgct ctctcgtact gaccgtcgag gctgtaggct
     3301 atggctctac tcatgagcac ataagggtct ttggggttga gggctactcc cttgttaaac
     3361 tcttcaatgg ccaagggcag cttatcgagg tcaaaatagc agcatcccag ttgctggtgg
     3421 cagtaagaat agttgggcag cagcctgaca atttccctgg ctacgccgag ggcaccggta
     3481 tcatcgccgg tgactttcaa gtgttccaat ttgtcgaaga ggggcttgca ctttctgtat
     3541 gcaggcaggt cctctgggct tggagcttgc agaccgatgt gtggcttgtt gatgtttata
     3601 ttggatttgc ccatgacgtt gttcaggtct tttgtcaggg ccgctcttcc cttttcgcta
     3661 ttttcgtagc gattgagggt gcttgccact tgcggcaggt agagttccag tagtttgtcg
     3721 ttgtctgtag cacccggtct tgtggccagt aagtgtgcat agaccttgag gtctttcagc
     3781 gagtcggcgc gcttgccgag gctgccgtaa caggcggcac gatggaagta ggcatagccg
     3841 ttctttggtt cgatgcttat ggcgtagctg taaacatcga tggctgcttg aaacttcttt
     3901 tcattgagga gggttccggc ttgtttgaag agagccagaa ctttcgggtt tttcccagcc
     3961 ggtagggcac atcgtacttt cacgggctcg gtctggggcg gctgagctgt gcagtcggcg
     4021 gttgtagcga gatttagcga aaagactagg ggcagccata gaagcagcgg cttcgctctt
     4081 ctgcctgtgt cggaggtctg ctgtcgaccg ggctcgcttg agaattgctt ggcttggctt
     4141 ccgggcaagt gctgttcctc ggtttttagc tggcagtttc aggctttact ttgagcatat
     4201 aacagaagct gaagacatag agcaagagag ctaccagaag caagttgctg ataccaaggt
     4261 aagtagagag atattccaat aaaccgccca gcacactgcc tagaagatta aaggcaaaag
     4321 agcgagaggg tttctcggct cctttgaaag ccgtggcgaa aattagaccg gccaggaaga
     4381 tgggagcgag ggtgacgatg gtcatggcg
//
LOCUS       DAOQNZ010000130         4393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8760_length_4393_cov_5.124020, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000130 DAOQNZ010000000
VERSION     DAOQNZ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8760_length_4393_cov_5.124020"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="PKW73_03440"
     CDS             <1..1777
                     /locus_tag="PKW73_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HNA72363.1"
                     /translation="SGVFVSVELEEKKGTASQSDCRKRLDRIFLELQKDADRAHEAKT
                     YREPSTVHGAARWGTDEELKAAKYIQKVADAKRLIIGRTPDNQYLQVPEFWTHAHSIV
                     CGRTGVGKSRGFFIPQLIERIGTSMIVTEATPGYEAGELYKLTSGWRKMAGHEIYCFN
                     PSDLTSNRINPIDRVRRAPEELKAQLAEKLADLVIMNGESTEAKGEQTWNRSEKLLLI
                     PLILHAAAGEPQFGHFGALRWLILQGPDRLEQILKRSPSDIAQMEFEGWMRLSGETDF
                     KYGVFSGLITKLNPWMTDQMVTLTETTDIDLEALKRQLFTFYLAVPSRSRDSKLIGSL
                     MVNFLLDHVLEIREEMKYPLTMLLDEFTNFGKISGIGDTLSIIRKNKIGLILGFQNYA
                     QLEQVYSRREAQIIIDMPATQVYFKQKTFKEAKELSEAIGRTTVEEATVSDSGRVQEF
                     VQGRALITPDELISLKDEVIVFTADTKPLRLPLTSPIAYEQALAYDAPVREKHEVSEF
                     VRKRGRTARSQQAEQKKDPREKRESKQEAKPKEQENRGGKKGTGRFERQKPPTEDKVK
                     ESDNEQEERKPEPKRDRYSGAPDFR"
     gene            1791..2324
                     /locus_tag="PKW73_03445"
     CDS             1791..2324
                     /locus_tag="PKW73_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72364.1"
                     /translation="MALKSETYLLLHKMLGAAETEEDLEIARKSFQAVVDESRDSESR
                     DERFDVAWSMSCLAGIYVRLEQMTLAEQAYLTAIRLFDENDMAAHSACLSVALAKLYA
                     EIGRAKDAHIQMKEYVHYETKDWGEGSEQAKDAQEELAHFECTGEFIQAFDHRWCKAC
                     GVDRYGVGFELDEDGRE"
     gene            2560..3159
                     /locus_tag="PKW73_03450"
     CDS             2560..3159
                     /locus_tag="PKW73_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72365.1"
                     /translation="MPQAFAKVRELLAELTYNFGDKWHVVTADVQTKRITANLRFTDE
                     EMKMEGDSRGQIHTRTERVQRLVELEVQMKETASGGTIVQFDFYPKIEGANISACDSV
                     VSGFCRGIEAAIGPGIERGTPADTRLPAPPWWLIGLTVCGVMSLFGSISARVGEIRQR
                     IDEHPRQLQQEKTNQEDREQAMRDEMAAWKKFKEGNNLR"
     gene            3156..4271
                     /locus_tag="PKW73_03455"
     CDS             3156..4271
                     /locus_tag="PKW73_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNA72366.1"
                     /translation="MNNLQQAYVDLGIPIGSSLEAVERRYKRLVMAWHPDRYPNEEHK
                     REAEEELKKINNAKDLLKEHLKGSQHKDAGECDCRMQAGAAEHAQSRQPGSSGPGRKA
                     KTTAETRQEEFEAKFRDAERSRRAQEEQERQERQRGEQAAEAAHEESVKTAFRQESQR
                     STESLRWKICLGSAAVSILLMFVPSIAYLVEQPILAGQNVIYGQHGESAESRKLAEEW
                     RSYQNDKAQVATSLLPQLSGGSDTTIWRPPFVSNDASADYISRQAYQELVLKVEEQKK
                     QHAMEVYKARSEVDRYQKAIERSQSVLADCAAKLADPNSALTDQRITLADQADHRRYL
                     QENQENLRAAQKRLDELEAESPGAVPLEPRFGPAQVQ"
BASE COUNT         1229 a          916 c         1335 g          913 t
ORIGIN      
        1 aagcggtgtt tttgtcagtg tggagctgga agaaaagaaa ggcacagcca gccaaagtga
       61 ctgccggaag cgcttagacc ggatttttct tgagctgcaa aaagacgcag accgggcgca
      121 tgaggcgaag acttacagag agccaagcac agttcacgga gccgctcgct ggggtactga
      181 tgaagagtta aaagcggcaa aatacatcca gaaagttgct gatgccaaaa ggctaattat
      241 tggcaggacg ccagataatc agtacttgca ggtgcctgaa ttctggaccc atgcgcactc
      301 gatagtttgc gggcgtactg gggttggcaa gtctcgtggg ttctttatcc cgcagttaat
      361 tgagcgtatc ggcacaagca tgatagtgac tgaggcgacg ccaggctacg aagctgggga
      421 gctgtataag ctcacttctg gctggcgcaa gatggccggg catgagattt attgcttcaa
      481 tccgtcggat ttgacctcga acagaataaa tccgatagac agggtgagga gggcgccgga
      541 ggagctgaag gcgcagttag ctgagaaatt ggccgatctg gtcataatga acggcgagag
      601 tactgaggct aaaggtgagc agacttggaa ccggtcggag aagctcctcc ttatcccgct
      661 aatacttcat gcggcagccg gggagccgca gttcgggcat tttggtgctt tgcgctggct
      721 catcttgcag gggccagata ggttagagca gattcttaag cgcagcccat cggatattgc
      781 gcagatggag tttgaaggct ggatgcggct gtccggcgag acagacttta agtacggggt
      841 attttcaggg ctaataacaa agcttaaccc gtggatgacc gaccagatgg tgacattgac
      901 cgagactacg gatattgacc tggaggcact gaagaggcag ctatttactt tctatttggc
      961 tgtgccgagc cggtcgagag atagcaaact tatcgggtca ctgatggtca acttcttgct
     1021 tgatcatgtg ctggaaatca gagaagaaat gaagtatccg ctgacgatgt tacttgatga
     1081 gttcacgaac ttcggcaaga tatcaggcat tggggatact ttgtcgatta ttcgcaagaa
     1141 caaaatcggg ctaattttag ggttccaaaa ttatgcacag cttgagcagg tgtacagcag
     1201 gcgcgaggct cagataataa tcgacatgcc agcgacgcaa gtttacttca agcagaagac
     1261 tttcaaggaa gcaaaagagt taagtgaggc gattggccgc acgaccgtag aagaagccac
     1321 cgtgagcgat tccgggcgcg ttcaagagtt cgtccaagga cgagctctca tcacgccgga
     1381 tgaactaatt agtctcaaag acgaagtgat agtttttact gccgacacaa agccgctcag
     1441 gttgccgctt acatcgccaa tagcatatga gcaggctcta gcgtatgacg cgccggtgag
     1501 agaaaaacat gaagtgagtg aatttgtgcg caaacgcggg cgcacagcca gaagccagca
     1561 ggctgagcag aaaaaggacc cgagagaaaa gagagaaagt aagcaagaag ccaagccaaa
     1621 agaacaagaa aacagaggag gcaaaaaagg cacaggacgg tttgagcggc aaaagccgcc
     1681 aactgaggac aaagtcaagg aaagtgataa cgagcaagaa gagcgcaagc cggagccgaa
     1741 acgcgacagg tattcaggag caccggactt ccggtagtga gaggtttttt atggcactga
     1801 aatctgaaac ttacttgctt ctgcacaaga tgttaggggc ggcagaaaca gaagaagacc
     1861 tggagatagc acgcaagagc tttcaagctg ttgttgatga aagccgggac agtgagagcc
     1921 gtgatgaaag atttgatgtt gcctggtcga tgtcgtgcct ggctggcatc tatgtccggc
     1981 ttgagcagat gacccttgca gaacaagcgt atttaactgc aatcaggcta tttgatgaaa
     2041 atgacatggc tgctcactca gcatgtttgt cagttgccct ggcgaagctc tatgcggaaa
     2101 taggcagggc taaagacgct catattcaga tgaaggagta tgttcattac gagaccaaag
     2161 actggggaga aggaagtgaa caagcaaagg acgcgcaaga ggaacttgcg cactttgagt
     2221 gcaccggtga atttatccag gcgtttgatc acaggtggtg caaagcttgc ggagtagatc
     2281 gctacggagt cggctttgaa ttggacgaag acggcagaga gtagcgaaga tgaaagctga
     2341 ggcgagacaa gagaaatgat cgttatcggg ataatacttg gcttgattgt ctggggagta
     2401 gttggctcag gcattaggta cttcgtactt cagacgccga tgacaggcat gttcgaggga
     2461 tttgtagcgg gagtgtgggc ggcgtggccg ttcattcatc agttcagggt cggcagatat
     2521 aactttctgc atcctgtgcc aaaagagtac aaagctggtg tgccgcaggc atttgcgaag
     2581 gtgagggagc ttcttgccga gcttacttac aatttcggcg acaagtggca tgttgtcaca
     2641 gcggatgtac agacaaagag aatcactgcc aatctgcgtt tcaccgatga agagatgaaa
     2701 atggaggggg attctcgggg gcagattcat accagaaccg agcgagtcca gcgcctggtg
     2761 gagcttgaag tgcagatgaa ggagacagca tccggcggaa caatagtgca gtttgatttt
     2821 tatccaaaaa tcgagggagc aaatatttca gcctgtgact cagtagtatc gggcttttgc
     2881 cgagggatag aggcggcaat agggccaggt attgaacgcg gcactcctgc cgatacgagg
     2941 ctaccggcgc cgccctggtg gctcattggt ctgacagttt gcggcgtgat gagtctattt
     3001 ggctcaatat ccgcccgagt gggagaaatt aggcagcgta tagacgaaca cccgaggcaa
     3061 ttgcagcaag agaagacgaa tcaagaagac cgtgagcagg ctatgcgcga tgaaatggct
     3121 gcctggaaga agttcaagga gggaaacaac ctcagatgaa taacttgcag caagcctatg
     3181 tcgatttagg tattcctatt ggatcatctc tggaggcagt tgagaggcga tacaagcgcc
     3241 ttgtgatggc ctggcatcct gaccgttacc ccaatgagga gcacaaaagg gaagcggaag
     3301 aagagttgaa gaagataaat aacgcaaagg acttactgaa ggagcacctg aaaggtagcc
     3361 agcacaaaga cgctggggaa tgtgattgcc gaatgcaagc aggagcggct gagcacgcgc
     3421 agtctcgtca gccgggatct agtggaccgg ggcgcaaggc taagaccact gccgaaacca
     3481 ggcaagaaga gtttgaggct aagttcaggg acgctgagcg aagcaggcga gcgcaagagg
     3541 aacaagaaag gcaagaaagg cagcggggag agcaggcggc tgaggctgcg cacgaagagt
     3601 ctgtaaagac cgcgttccgc caagaaagcc agagaagcac ggagtctttg cgctggaaga
     3661 tttgccttgg cagcgctgct gtttccatac ttttgatgtt tgtcccatcc atagcttatt
     3721 tagtcgaaca gccaatctta gccgggcaga acgtaattta cgggcagcac ggcgaaagtg
     3781 cagaaagccg caagttggca gaagagtggc gcagttacca gaacgacaag gcgcaggtgg
     3841 cgacttcgct tctgccgcag ctatctggtg ggagcgatac aacaatatgg cggccgccgt
     3901 ttgtgtcaaa cgatgcgagt gccgactata tttcgaggca ggcgtaccag gaacttgttt
     3961 tgaaagtgga agagcaaaag aagcagcatg ctatggaggt ttacaaggcc aggagcgaag
     4021 tagaccggta tcaaaaggca attgagcgca gccagagtgt gctcgcagat tgtgccgcta
     4081 aactagcaga tcctaattca gctctcacgg accagaggat aacgctggca gaccaggcgg
     4141 accaccgccg ctacttgcaa gagaatcagg agaatctgag ggcggcgcaa aagcgtcttg
     4201 atgaactgga ggcagaaagt ccaggggcag ttccgttaga gccgaggttt gggccagcgc
     4261 aggtccagta attgaggatc ggtggttgac tttgggtact atagtagaaa ttatgccgtt
     4321 gatatgcgtc tgattgtttg taagtttcag aattatttgt cgcacttttt gcagaattat
     4381 ttgtcgcggt ttt
//
LOCUS       DAOQNZ010000131         4390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8772_length_4390_cov_4.150634, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000131 DAOQNZ010000000
VERSION     DAOQNZ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8772_length_4390_cov_4.150634"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PKW73_03460"
     CDS             <1..597
                     /locus_tag="PKW73_03460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA72367.1"
                     /translation="SAAGFWAVFRSPGVMTLIILAAFFSTFGMQYSVLMPVIVDKILH
                     GSSEQFGFLSAAGGLGSLMGALLIASFGSRTGLRRRLGLAAVVLSAAVLLLAFSQVFA
                     LSVVAIMICGACLSMHWSGGNSLIQQCAEPGKRGRLMGAYSTFTLGLAPFTALIAGWT
                     AEHFGVGSALMFASAGMFVGACIYLFKSRKLDDTCEGD"
     gene            896..1264
                     /locus_tag="PKW73_03465"
     CDS             896..1264
                     /locus_tag="PKW73_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72368.1"
                     /translation="MADNSASGTEGKKASTDVLSAAFTIYMEAEKPTKRDFVKKLGAA
                     VPGLDAKEYEQAWTRVSNLFEQSCKLAFRWANENSPGSEIDISLIENVFINEIARKCQ
                     GFTYAEYSKALTYGFDRGIF"
     gene            complement(1305..1970)
                     /locus_tag="PKW73_03470"
     CDS             complement(1305..1970)
                     /locus_tag="PKW73_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA72369.1"
                     /translation="MSPTSPQPTDKLAGTLRHRLLLAIETLRVVDKVMLGKALSLAEQ
                     VHSGQTRRPARNVSNPKHASPFIVHPMRVALIILEELELKEPLAVAAALLHDTVEMSA
                     GKVTIGSIEEAFGRPIAMMVSILSMPPLAANASKQERDRILKIYQDRIFNASVETKLV
                     KLADRLDNTREAAELLDKSFQQKMLDEGKSFYLPLAETTDAYLMEELSLALEALEKRI
                     KFG"
     gene            complement(2212..3297)
                     /locus_tag="PKW73_03475"
     CDS             complement(2212..3297)
                     /locus_tag="PKW73_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNA72370.1"
                     /translation="MNCKNSCPAVRQVMFPKDCDPSGNIFGGIILSHIDIAGAIAAKK
                     LTVHRLVTVSMDTIVLKKPVLVGDILTCWAEVVEKGRTSLKVKITVESERQGQVIPVT
                     EGYAKYVAVDKEHRSIPWDSPAGTVAQDSAAPVRARVEPKSLTKPILLQLAENDLSSA
                     ARRCPAVRQAMFPKDTNPSGNIFGGIILSHIDIAGAIAAKQLTEHRVVTVAMDTVIFK
                     KPVLIGDILTCWTEVIKKGRTSIQVKVTVEAERHGEIISVTEGNATYVAVDKDHKPVP
                     WNTPLGTTADQVASGEVPVPAHDSDSEQDCGCNHGPVQPEAEPVEGATKKNKKKKGKD
                     KPKEDKKKKKNKKEKKGKKKKRGKKNK"
     gene            complement(3347..>4390)
                     /locus_tag="PKW73_03480"
     CDS             complement(3347..>4390)
                     /locus_tag="PKW73_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72371.1"
                     /translation="PLWVVDGHEVGGHGIHTVVNGFDSEVAAALKQCVRKSTAKIKNL
                     PERVTKESLALLLLSGDDKNEFAAAELLELLAGSYSQELAADLAGLLNFGPMFTNGLM
                     LYFIAHHKEGTLRNKGRLLPSKEQERMDAHPPDVLRALISIKAVSKLRLDKAQLTTLV
                     ADLTARLKEACGGTLPADLVFADEADATAVTLPLKYFEPLIEDIAECLMNEPLKCLGD
                     RPLSQVLTWTQMDQDISERLAQTMMLTSFDHKAETDLEARHLVAASLMALETIAIGAG
                     NQRKDGKRLFERIQANSVHALKDFYLEQCLLCAVPTYSKSRRTETSLKGKTVDSLIKA
                     LKRSRLHMKQPGD"
BASE COUNT          820 a         1142 c         1300 g         1128 t
ORIGIN      
        1 agtgcagccg gtttctgggc tgtctttcgc agccccggcg tcatgaccct gatcattctg
       61 gcggctttct tcagcacctt cggtatgcag tactcagtgc tcatgccggt catcgtggac
      121 aagatcttgc acgggtcttc cgagcaattc ggcttcctct ccgctgccgg tggtcttggc
      181 tctttgatgg gggcgctctt gattgcttcc ttcggctccc gcaccggcct gcgtcggcgt
      241 ctgggtctgg ctgcggtcgt gctttctgcg gcggtgctgc tgcttgcctt ctcgcaagtc
      301 ttcgccctgt ccgtggtggc aatcatgatc tgtggtgcct gtctttccat gcactggagc
      361 ggcggtaatt cgctgattca gcagtgcgcc gaaccaggca aacgcggtcg actgatgggg
      421 gcttattcca ccttcaccct tggcctggcg cccttcacgg ctctgattgc cggttggacc
      481 gccgagcatt ttggtgtggg cagtgccctc atgtttgcca gtgccggaat gttcgtaggt
      541 gcttgcatct acctgttcaa gtcgcgcaag ctggacgaca cctgcgaagg tgattgatgt
      601 ctacaggcgc taccggttga atacttgagg gagaccggaa aggaataaaa cacttttccg
      661 gtctccttct tgctttcacg atgccgcttg catttctctt tgccgtttga tactaaggaa
      721 tatcgtataa ttgacaaatg tgttttctgg tctttttctg ggcaaataga gggcaagcgt
      781 ataattgccg ctaagattga aaagtacgct tggaataccc ggaataccga ccctggcttg
      841 atgtgatcga ttgaaaatat cgcccggcgg aaagaggaag gagtgaatct gggtcatggc
      901 agataatagt gcaagtggaa ctgagggcaa gaaagcttcg actgatgtgc tcagcgccgc
      961 atttaccatt tacatggagg ctgaaaagcc caccaagaga gatttcgtca agaagttagg
     1021 ggctgccgtg cccggtcttg atgctaaaga gtatgaacag gcctggaccc gggtcagcaa
     1081 cctatttgag cagtcctgta agttggcttt tcgctgggct aatgaaaaca gtcccggctc
     1141 ggagattgat atttccctga tcgaaaacgt cttcatcaat gaaatcgcca gaaagtgcca
     1201 gggcttcact tatgccgagt actccaaggc tctcacctac ggttttgacc ggggcatttt
     1261 ctaagcaaga tgcaaaccaa gcagcaaacc aagcagcaaa ctaattagcc gaatttgatg
     1321 cgcttttcca gcgcttcaag agccagggac agctcttcca tcaggtaagc atcggtagtc
     1381 tcggccaggg gcaggtagaa actcttgccc tcatcgagca ttttttgttg gaaggactta
     1441 tcgagcagtt cggctgcctc tcttgtgttg tccaggcggt cggcaagctt gaccagtttg
     1501 gtttccacac tggcgttgaa aatacggtcc tgataaatct tgagaatccg gtcgcgctct
     1561 tgtttggaag cgttagccgc caggggcggc atgctcaata ttgagaccat catggctatg
     1621 gggcgcccga aggcttcttc gatagagccg atagtgacct taccggcgct catttccaca
     1681 gtgtcgtgta ggagggcggc ggccacggcc aggggttctt tcaactccag ttcttccaga
     1741 atgatcaagg ccacgcgcat ggggtgcacg atgaagggag aggcatgttt tggattactg
     1801 acgtttctgg ccggccttct agtctgaccg gagtgcacct gttcggccag gctcagggct
     1861 ttgcccagca ttaccttatc taccacccgt agcgtctcga tggctagcag aagcctgtgt
     1921 cttaaggtgc ctgccagttt gtccgtcggt tggggagaag tggggctcat cttgggatat
     1981 ccgattggtg gtctattagt gtagctgttc tctcattgca tatgcccgat tatcagtctt
     2041 aaccatccgg cagtttgtat tttttcaaac cggcttcttg aaagaaacta taggaaataa
     2101 tagaaaatga aaaaaagaga gagcccggtt gaggggctct ctctcgattt gcgacaggtg
     2161 aggggctttg tcggcaatct ttgaagcaag ggctggacct ggacggcgcc cttacttgtt
     2221 cttcttgccg cgcttctttt tcttgccctt cttttccttc ttgttcttct tcttcttctt
     2281 gtcctctttg ggcttgtcct tgcccttttt cttcttgttt ttcttcgtcg caccttccac
     2341 gggctccgct tcgggctgca ccgggccgtg gttgcagccg cagtcttgct cggagtccga
     2401 gtcatgtgcg ggaacgggga cctctccgga ggctacctgg tcggccgtgg tgcccagggg
     2461 cgtgttccac ggcacaggct tgtggtcttt gtccacagcc acataggtag cgttgccttc
     2521 ggtgacagag atgatctccc cgtgccgctc ggcttccacg gtgactttca cctggatgga
     2581 ggtgcgacct ttcttgatca cctcggtcca gcaggtgagg atgtcgccaa tcaggacagg
     2641 cttcttgaag atgaccgtgt ccatggccac agtgacgacg cggtgctcgg tgagctgttt
     2701 ggcggcgatg gcgccggcga tgtcgatgtg gctcaagatg atgccgccaa agatgttgcc
     2761 cgatgggttg gtatctttgg ggaacatggc ttgtctgacg gccgggcagc gcctagcggc
     2821 actgctcaag tcgttttcgg caagctgaag gagaatgggc ttggtgaggg acttgggctc
     2881 aaccctggct ctgacggggg cagcgctgtc ttgcgccacc gtgccggctg gggaatccca
     2941 gggaatggag cgatgctcct tgtccacagc cacgtatttg gcgtagcctt ccgtgaccgg
     3001 gatgacctgg ccctggcgct ccgattcaac agtgattttc accttcaaag aggtgcgtcc
     3061 cttctccacc acttccgccc agcaggtgag aatgtcgcct acgaggacgg gctttttcag
     3121 gacgatggtg tccatggaga cggtgacgag acggtgcaca gtcaactttt tggcggcgat
     3181 ggcgccggca atgtcgatgt ggctcaagat gatgccgccg aagatgtttc cggatggatc
     3241 gcagtccttg ggaaacatta cttgcctgac ggccgggcag gagtttttac agttcatata
     3301 gcttttcctg tggaaaacgt tggcagaacc gatggaattg gcagactcag tcaccgggct
     3361 gtttcatgtg caagcgcgaa cgcttgagag ccttgatgag ggaatccacg gtcttgccct
     3421 tgagggaagt ttccgttcgg cgtgatttcg aataagtggg cacagcgcaa agcaggcatt
     3481 gctccagata gaagtctttg agggcgtgca cgctgttggc ctggatgcgc tcaaaaagcc
     3541 gctttccatc tttccgctgg ttgcctgcgc cgatggcgat ggtttccagg gccatgaggg
     3601 aagctgccac cagatgccgt gcttccaggt cggtttcggc cttatggtcg aagctggtca
     3661 gcatcatggt ttgagcgagg cgctcgctga tatcctggtc catttgggtc caggtgagca
     3721 cctgactgag gggtcggtca cccaggcatt tgagcggctc gttcatgagg cattcggcga
     3781 tgtcctcgat gagcggctcg aagtacttga ggggcagcgt gacggctgtg gcgtcggctt
     3841 catctgcgaa aaccagatcg gcgggcagag tgcctccgca ggcttctttc aggcgtgccg
     3901 tcaggtctgc caccagggtt gtgagctggg ctttgtccag cctcaacttg gagaccgctt
     3961 tgattgaaat gagggcacgc agcacatcgg gtggatgggc gtccattcgc tcctgttcct
     4021 tgctcggcaa gagacggccc ttgttgcgca gggtgccttc cttgtggtga gcgatgaagt
     4081 agagcatgag accgttcgtg aacatcggtc cgaaattgag gagaccggcc agatcggctg
     4141 ccagctcctg ggagtaactg cctgccagaa gctccagcaa ttccgccgct gcaaattcat
     4201 tcttgtcgtc acctgatagc aggagcagag ccaggctttc tttggtgacg cgctcgggca
     4261 ggtttttgat cttggctgtg ctcttgcgca cgcactgttt gagcgcggca gctacctcgc
     4321 tgtcgaagcc gttcaccacc gtgtggatgc cgtgacctcc tacttcatgt ccgtccacca
     4381 cccagagggg
//
LOCUS       DAOQNZ010000132         4384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8795_length_4384_cov_3.445599, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000132 DAOQNZ010000000
VERSION     DAOQNZ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8795_length_4384_cov_3.445599"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PKW73_03485"
     CDS             complement(<1..712)
                     /locus_tag="PKW73_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNA72372.1"
                     /translation="MQKQLENTPGTASIALDEPAPGSTRTKLHQSEDFYVDLDENGIA
                     GLWFACTKNANVLGQSTLDQLKIILDLLKKDTKVRGILLASAKKDHFVYGADLHEILG
                     FKTFEQAHFLSSEGQKVFQELATLGKPVVAALHGACLGGGLEAALAATRRIASDHEST
                     LLGLPEVRLGLIPGLGGTQRLPRLIPLRNALEFILTSEAVGAARALELGIVSEVVKHE
                     DLFKRARDLALELVKGTAP"
     gene            complement(741..1895)
                     /locus_tag="PKW73_03490"
     CDS             complement(741..1895)
                     /locus_tag="PKW73_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72373.1"
                     /translation="MAKEAKKSKKEGKKPSAAQANLPEQNTKANLPGPPPPVATSKTT
                     AATLQLAGDTDSGPYRPGQCVICRVEGIEPGGYSAVIQSSVPPSEDDKDAAHHIRAFL
                     PSTDTLKIGQTVPATFVCMHNNRALMTFAFMLGTTEKIQKSTATDAENAFSIWVDSYP
                     STQKARRAVDLIMPSVSGKLLRELKCASEETTRLLQELELAKFTGCIKARNEAKKSRS
                     AMLIVEGRIVGAIYGRKDAHETFAVERAIELMREDILDGETFLQVYELPQAAVLSMSA
                     LFLGCPIKSDSATVRDYLDTSLMSMSLAGETGCITYSADGKNTDSLIFIHEGQPLAGY
                     EIAEQKYLEEPAALIKQLKELEAGQVEAHLLPTQLLSTSVKFGYKLTAEN"
     gene            2156..2767
                     /locus_tag="PKW73_03495"
     CDS             2156..2767
                     /locus_tag="PKW73_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72374.1"
                     /translation="MIQDEYKESASKDGNSQGLRVSKDSVGTALLAKLSPEDVEPVAA
                     DADRQLVDGWICGRDPRTFKSPYRTVSPREKALFALAYRDFIAGRGQNLGLSLSTYIP
                     YQDRSRFARQYWEKSALKEEFAKETEKYQVCWIHESLLDGSELVFRAAERKFGQSLDS
                     ALIMAHDVEQNMVSKYMADERTESSFAMQFKPKSGTGEKESAR"
     gene            2822..3526
                     /locus_tag="PKW73_03500"
     CDS             2822..3526
                     /locus_tag="PKW73_03500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNA72375.1"
                     /translation="MTDNRQKRFSEKEEREKLFQRRLAELRAKNNQFPWGLSESADFE
                     IPAEQSKLFADRYIEDPSLTDDEVERRALLDLNSFNFWTFYKRLNYEVKRAIRYKRPL
                     SLLFVTIDRLARVVENHGLNGENAVILACGKVLLGCVRDVDVAGRCRDDSFGIILPET
                     PRAGAEIAAERIRTRMEDLGVDIGSTTIFCTVTVGGASLEEGLDKPDQLIALTVQGLK
                     QGLSAGGNSVHFPVAL"
     gene            complement(3559..>4384)
                     /locus_tag="PKW73_03505"
     CDS             complement(3559..>4384)
                     /locus_tag="PKW73_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72376.1"
                     /translation="LGAPTIGEVKEIPENVMNFVAAEAHYLKANNFVSSGNFDEALKE
                     YEAASIVDPTYADAIGDWGAVLAIKGDWSGACAKYKKAIELKPNDALFRANYARALTK
                     SGQEEEGIKQLEMAYQLNPRDRIILSALAGKCIEAGNFDTAINLLEQGTFLFPQSAQM
                     HDKLSYVFAKAGNYSQSLAHAREAVKLDPKLVSAKINLGSALLMKGNCQEAKSIYQET
                     TSMDPKNADAHFLLATTMERLGDTSGARTELTTFLELASKSDPRLTKAKEKLDSLK"
BASE COUNT          897 a         1058 c         1266 g         1163 t
ORIGIN      
        1 ccggagctgt tcctttgacc agttccagtg ccagatcgcg ggcgcgcttg aagaggtctt
       61 cgtgcttcac aacttcgctg actataccca gttccagtgc ccgtgcagct cccactgcct
      121 cagaggtgag gatgaattcg agggcattgc gcagcggaat gaggcggggc aggcgttgag
      181 tgccgcccag acctggaatg agaccgaggc gcacttccgg cagaccgagc aaagtgcttt
      241 cgtgatcgga ggcaatgcgg cgtgtggccg ccagggccgc ttccagtccg ccgcccaggc
      301 aagcaccatg cagggctgct accactggtt tgcccagggt agctagttcc tggaaaacct
      361 tctgaccttc ggacgagaga aaatgggctt gttcgaaagt cttgaaacca agaatttcat
      421 gcaggtcggc gccgtagacg aagtggtctt tcttggcgga agccagaaga atacctctga
      481 cctttgtgtc ctttttgagc aggtcgagga tgattttcaa ctggtcgagg gtgctttgcc
      541 caaggacgtt ggcgtttttg gtgcaggcaa accagagccc ggcaatgccg ttttcatcta
      601 gatcgacgta gaagtcttcc gactggtgga gctttgtgcg agtgctgccg ggtgccggct
      661 catccagagc gatgctggcg gtgccgggtg tattctcgag ctgcttttgc attttataga
      721 cttccgtgtg ttgttttatt ttagttttcg gcggtcaact tgtagccgaa ttttactgag
      781 gtagacagca attgagtggg caaaaggtgt gcttcgactt gccctgcttc cagttctttc
      841 aactgtttta tcagggcggc tggttcttcc agatatttct gctcggcgat ttcgtagccg
      901 gccagaggct gaccttcgtg tataaagata agtgagtcgg tattcttgcc atcggcagaa
      961 taggttatgc agccggtttc accggcaaga gacatagaca tcaaagatgt atcgagatag
     1021 tctcgcactg tggctgaatc gctcttgatc ggacagccca gaaaaagggc cgacatggaa
     1081 agcactgctg cttgcggcaa ttcgtagact tgcaaaaagg tctcgccgtc tagaatatct
     1141 tcgcgcatga gctcgatggc tctttctact gcgaaagttt cgtgagcgtc tttgcgtccg
     1201 tagatggcgc ctacaatgcg accctccact atgagcatgg cactgcgtga tttcttggct
     1261 tcgtttctag ctttgatgca gccggtgaac ttggccagtt ccagttcctg taaaagtctg
     1321 gtggtctcct cgctggcgca tttcaattcg cgcagcagtt tgccggaaac tgaaggcatg
     1381 attaagtcaa cggcgcggcg cgctttctgg gtggaaggat agctgtccac ccagatggaa
     1441 aaagcattct ctgcatctgt ggcagttgat ttttgaattt tctcggtggt accaagcatg
     1501 aaggcaaagg tcatgagggc ccggttattg tgcatgcaca caaaggtggc cggaacagtt
     1561 tgacctattt tcagggtatc ggtgctgggc aaaaaggcac gaatgtgatg ggcggcatct
     1621 ttgtcgtctt ccgagggcgg cacgctgctt tgtatgacgg cgctgtagcc tcccggctcg
     1681 atgccctcca cgcggcagat gacgcattgg ccgggacgat aggggccgct gtcggtgtcg
     1741 cccgctagtt ggagggtggc ggcggtggtc tttgatgtag ctaccggcgg tggcggaccg
     1801 ggaaggtttg ctttggtatt ctgctcaggc aggttggctt gcgccgcaga gggcttcttc
     1861 ccttctttct tggatttttt cgcttctttt gccacggttt ttgctcagtt gaagttcgga
     1921 ttatgaaaga atcaccactg atgaccggcg ccatctaatt gcttgtggct tagtttatca
     1981 tttgcattgt aggcagcgct tcaggctgtt gtcagaaggg tgtaaagtgc aacatttgac
     2041 gcgttttggt ttcatgaagg caaaggatgg cctgaggcca gtcttttata agtgccttaa
     2101 aaaggctgct gcgaccgctg ttatgggctt ctcaatttgc tccttgacag cctgcgtgat
     2161 tcaggatgag tataaggagt cggcatcaaa ggacgggaac tcacaaggct taagagtttc
     2221 gaaagattct gtcggcaccg ctctgcttgc caaactaagc ccggaggatg tggagccggt
     2281 ggctgccgac gccgaccggc aacttgtgga tggttggatt tgcggccggg atccgcgtac
     2341 ttttaagtcg ccctaccgca ccgtttcgcc acgggagaag gctctttttg ctctcgccta
     2401 tcgtgatttt atcgccggtc gcgggcagaa tttaggtctt tctctctcta cctatattcc
     2461 ttatcaggac cgttctcgct ttgccaggca gtactgggag aagtcggctt tgaaagagga
     2521 attcgcaaag gagacggaga agtatcaagt ctgctggatt catgaatcac ttctggatgg
     2581 ttcggaatta gtcttccgcg ccgccgaaag aaagtttggt cagagccttg attcggcgct
     2641 gataatggcc catgatgtgg aacaaaatat ggtgtctaaa tacatggctg atgaacgcac
     2701 cgaaagctcc tttgccatgc agttcaaacc aaagtccggc accggtgaga aagagagtgc
     2761 ccgctagggg ttgctccaga tcctcgctaa aagttcgcat aaggaaggaa gaattaagtt
     2821 cgtgaccgac aacaggcaaa agcgattttc agagaaggaa gaaagggaga agctctttca
     2881 gcggcgcctg gcggagctga gagcgaagaa caaccagttt ccctgggggc tttccgagtc
     2941 agccgatttt gaaataccgg cagagcaatc caagcttttt gccgatcgtt atattgaaga
     3001 tccatcactg accgatgatg aagtagagcg gcgggctctt ttagacctca attctttcaa
     3061 tttctggacc ttctacaagc gtcttaatta cgaggtgaag cgggctattc gctacaagcg
     3121 tcccctttct cttctcttcg tcaccatcga caggttggca cgtgtggtgg aaaaccacgg
     3181 gctgaatgga gagaatgccg ttattctggc ctgcggcaaa gttctgcttg gttgcgtgcg
     3241 cgatgttgat gtggccggtc gttgtcgtga cgacagtttt ggtattattt tgcctgaaac
     3301 tcctcgcgcc ggtgcggaaa tagcggctga acgcatccgc acccgcatgg aagatcttgg
     3361 cgtggatatt ggttctacca ccatattttg caccgtcacc gtgggtggtg ctagcctgga
     3421 agaagggctc gataagccgg accaactgat cgctcttacg gtgcaagggc tcaaacaagg
     3481 gctttcagcc ggcggcaatt cggttcattt tcccgtcgct ctctaaaagc actgcctcct
     3541 taagcgtctg gttttatctt actttagaga atccagcttt tctttggctt tggtaagccg
     3601 cggatcgctc ttgcttgcca gctcaaggaa ggtggtcagc tcggtgcggg caccgctggt
     3661 atcgcccagc ctctccatgg tggtagccag aaggaagtgt gcatctgcat ttttaggatc
     3721 cattgaggtg gtttcctgat aaatgctttt ggcttcctgg caattgcctt tcataagcag
     3781 ggcggagccg agattgattt tggctgagac caatttggga tcaagcttca ccgcttcccg
     3841 ggcatgggcc agggattggc tgtagttgcc ggctttggcg aagacatagc ttagtttgtc
     3901 gtgcatctgg gcggactgcg ggaagaggaa ggtgccttgc tccaggaggt tgatggccgt
     3961 gtcgaagttt ccggcttcga tacatttacc ggccagggca cttaagataa ttctgtctct
     4021 gggattcaac tgataagcca tctccagttg tttgatgcct tcctcttcct gaccgctctt
     4081 ggtgagggcg cgggcatagt tggctctgaa gagtgcatca ttgggcttca gttcgatggc
     4141 tttcttgtac ttggcgcaag ccccgctcca gtcacccttg atggccagca cggcgcccca
     4201 gtcgcctatg gcatcggcat aggtggggtc gactatggac gccgcttcgt actccttgag
     4261 cgcttcatcg aaattaccgc tgctgacgaa gttgttggcc ttcaaatagt gagcttccgc
     4321 tgccacgaag ttcattacgt tttcaggaat ttcttttact tcgccgatgg tgggagcgcc
     4381 caat
//
LOCUS       DAOQNZ010000133         4380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8803_length_4380_cov_4.015954, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000133 DAOQNZ010000000
VERSION     DAOQNZ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8803_length_4380_cov_4.015954"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..807
                     /locus_tag="PKW73_03510"
     CDS             190..807
                     /locus_tag="PKW73_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72377.1"
                     /translation="MWRVSLAEEAVFGCSKLILVSTFQRPLDGPCPPGYPGHRTLAND
                     FQGRSIVAFNQKINGFQHLYGSALVAFELGDAFASLLFAANEFAEFSFDFDGVRAEDL
                     LDRRKDLVNNRTGRKLGRQLAAGHLRGGRAERVLIAMSVTCMEESPEFLPHFMDQRLL
                     RAPDEASLGCPFLPKRNLFNWLLAGERAGIFRGCIKQVTRAVHHW"
     gene            complement(785..2893)
                     /locus_tag="PKW73_03515"
     CDS             complement(785..2893)
                     /locus_tag="PKW73_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72378.1"
                     /translation="MASFQTRRTPTFITTLAAALILPLLPSATPAPTVNLSFCPAAAA
                     QGITPQPMPRQFLERYNQGVSYMKSGEVDKALQIFKQVAINAPYDPLVHLSLAEAMHQ
                     SGDTDNAIAEYRRALRIRPYDAFARMSLGSLLQARGELQDAVNEYDEAIKLRPDVVLF
                     RLNLGIALRIAGSRDDAITELKKVVQAYPEHLKARAQLAAAYQDKGNFQMASEQYKKI
                     LQSQPQNFTMQFNQGACLLAMKDYGGAAEALSKAVKIDGSVAEAHALLGQTEVARGRL
                     PEAISELRGALAIKQDRADWYAQLGDALSKQKDFTGAIEAYRQSQNLNPQDTATGYSL
                     GYVLQTTGQLEDAARAFEHVVKLNPNYTNAHYNLGIIKQKQKDLPAAEAEFKRVLALN
                     PNDGQAYINLGRTLLLQGDLNQAVGFYRHGLSIEPTDPAAFQELGAALVKLKDYVGAR
                     SALESALGLAPNDRSAIISIANLESLEGTPSKGEFRIRQLLTNAPNDTELLNLLADNL
                     IQQGKVDAAIESYQRIIQLTPRSAEAFNDLGLALSRKDDVSGASAMYKKAIELDSQFV
                     EPLINLGNVYLGLRKYEEAGAQYQKALALKPDSVLAHYNMGLVETGKENMENALTEYQ
                     KAVQLDKTYANAFYAMGCLYQARNETEQALSNYEKYLSLEPSGPYAGNVKEAIEKIRP
                     ATAPTTAPETAGETTSGAPL"
     gene            3691..>4380
                     /locus_tag="PKW73_03520"
     CDS             3691..>4380
                     /locus_tag="PKW73_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA72379.1"
                     /translation="MAVYKRELQRKRFPKVERTAGVTNYEELAARALGNSINHLNNAY
                     SQKLSGAEGAVEFRDEGVYTYDNQGRRYLDCLGGFGIFNVGHRHPKVINAVKAQLDQV
                     ALHSQELINPWAAHLASQLAAVAPGDLQYCFFGNSGSEAVEGAIKLARLYTGKTEIIS
                     TKNAYHGVSMGALSATGRDVFRKPFEPLLNGFVHVPFGDIAALEAAINENTAAVILEP
                     IQGEGGINVPPT"
BASE COUNT          868 a         1080 c         1267 g         1165 t
ORIGIN      
        1 gcttgcgtaa tagcttttgt tctaagtcgc cgaggtgtgt ttctataaat gagatcgttt
       61 gtgctgaaag agctgctttt gccttgtctt ccgctgcggc aaggaagatg ctggaatata
      121 tggtgcaagg cgttgtgcct gtgttttctt tctagctttg tccttttggc gggttatgtc
      181 ctttctgttt tgtggcgtgt cagcctggca gaagaggcgg tttttggctg ttccaagctt
      241 atacttgttt cgacatttca gagacccctc gatggtccct gtccgcccgg ctatccgggt
      301 caccgtaccc tggctaatga ctttcaaggg cgctctattg tagctttcaa tcagaagatc
      361 aatggctttc agcatctcta tggttcagcc ctggtggcct ttgaacttgg agatgctttt
      421 gcctcgctgc tctttgccgc taatgagttt gctgaattta gctttgattt tgatggggtg
      481 cgggccgaag acttgctgga ccgccgcaag gaccttgtca ataatcgcac cggtcgaaag
      541 ttggggcgtc agctggccgc cgggcatctg cggggcggta gggctgaacg agttctcatc
      601 gctatgtcgg tcacctgtat ggaagagtcg cccgagttcc ttcctcactt catggaccag
      661 cgccttttgc gcgctcctga tgaagcctct ctcggttgtc cgtttttacc gaagcgcaac
      721 ctgttcaatt ggcttctagc tggtgaacgc gccgggatct ttcgaggctg tataaagcaa
      781 gttactagag cggtgcacca ctggtagttt cacctgccgt ttccggagcg gtggtcggtg
      841 ctgtggcggg gcgtatcttt tcgatcgctt ctttgacgtt accggcatag ggaccgcttg
      901 gttccaggct caaatacttt tcgtaattgg agagtgcttg ctccgtttca ttgcgagcct
      961 ggtagaggca gcccatggcg taaaaggcat tggcataggt cttatccagt tgcacggctt
     1021 tctgatattc ggtgagggcg ttttccatgt tttctttgcc ggtttccacc agacccatgt
     1081 tgtagtgggc cagtacgctg tcaggcttga gtgccagggc tttctggtat tgagcaccgg
     1141 cttcctcgta ctttctcaga cccaggtaga cattgcccag gttgatcaaa ggttccacaa
     1201 actgagaatc aagttcgatg gctttcttgt acatggcact ggcgccggag acgtcgtctt
     1261 tgcgtgagag tgccagaccg aggtcgttga aggcttcggc ggagcgcggt gtcaactgga
     1321 tgatgcgctg ataagattcg atagccgcgt ctaccttgcc ctgctgaatg agattgtctg
     1381 ccagaagatt gaggagttcg gtgtcgttgg gggcattggt gagcagttgt ctgattctga
     1441 attcgccttt ggagggtgtg ccctccaggc tctccagatt ggcgatggag attatggcgc
     1501 tgcgatcgtt gggagccagg ccgagcgcac tttcaagagc agagcgggcg ccgacgtaat
     1561 ctttcaattt gactagcgct gctcccagtt cctggaaggc agccgggtct gttggctcga
     1621 tgctgagacc atggcggtag aaacccactg cctggttcaa gtctccttgc aagagcaggg
     1681 ttcgaccgag gtttatgtat gcctgcccat cattgggatt gagggccagt actcgcttga
     1741 actcagcttc cgcggcgggc aggtccttct gtttttgttt gatgatgccc aggttatagt
     1801 gtgcgtttgt gtaattggga ttgagtttga ccacatgttc gaaggcccgg gcggcgtctt
     1861 ccagttgacc tgtggtctgt agcacgtaac cgagactgta gccggtggct gtatcttgcg
     1921 gattgaggtt ttgagactgc ctgtaagctt cgatggctcc tgtgaagtct ttttgtttgc
     1981 tgagggcgtc gcctaattga gcgtaccagt cggcacgatc ttgctttatg gccagggccc
     2041 ctctaagttc gcttatggct tccggcagcc ggccgcgcgc gacttcggtt tgccccagaa
     2101 gggcatgggc ttcggctacg gagccgtcta ttttgacggc tttcgagaga gcctcggccg
     2161 cgccgccgta gtctttcatg gctagcaaac aggcaccctg gttgaattgc atggtgaaat
     2221 tctgaggttg tgactgcaaa atcttcttgt attgctctga ggccatctga aaattgcctt
     2281 tgtcctggta ggcggccgcc agttgggcgc gtgctttcaa atgctcaggg taggcttgaa
     2341 cgactttctt caattcggtt atggcgtcgt cgcggctgcc ggcgatgcgc agggctatgc
     2401 ccagattgag gcggaagagc accacgtcgg ggcgcaattt tatggcttcg tcgtattcgt
     2461 tgacggcgtc ttgcagttcg ccgcgggctt gtaagaggga gcccaggctc atgcgggcaa
     2521 aggcgtcata ggggcggatt ctcaaggcgc ggcggtattc ggcgatggca ttgtccgtat
     2581 cgccggactg gtgcatagcc tcagccagac tgaggtgcac cagcgggtca taaggagcgt
     2641 tgatagccac ctgtttaaag atttgcagcg ctttatcgac ttccccggac ttcatgtaag
     2701 agacaccctg gttgtagcgc tccaggaact gccggggcat gggctgtgga gtaatcccct
     2761 gggccgcggc ggcggggcag aaactcaggt tcactgtggg cgccggcgtg gcacttggca
     2821 ggagcggcaa gattagggcc gcagccagtg ttgttataaa tgtaggggtt cttcttgtct
     2881 ggaatgacgc cattatctgc tttggcatga accttaatct cttcctttat tgtcaagcgg
     2941 cttcgggagc ccaattcaat cataattatt gcccaagaga cgccagtttc caaagtaagt
     3001 tcctcttacc ttgatcggcg ccctgaagcc ccggaacccg cacaggggcg gtagtcaagg
     3061 ggtgaagggc gaacatgttc ccgtttttgt cttagacagg ttggctaaat tctgtcgccg
     3121 gtttttgtac aggttgcaca ttgttctgaa tgtgacagcc ggtgtacatt agagatatag
     3181 aggcgaaggg aagttagagg gacgctcaca ggcaaacatg cttctgtagc gcttatctcg
     3241 cggttgaacc acttaaatca attcaacacc gggtccctgt tccaccagca aaaggctgaa
     3301 cgaaccactt tccaaagttt agcgcttcta gacacggcat ccactgaccg ttgtttgttg
     3361 attccaaaga gaataggttg tttatgagag ctgtttttgc agcaaaacct gaaaaatctt
     3421 ccagagcgag agcttaccag tctacttatc tgtagcgggc ctgagatcaa tagacagagc
     3481 ctcgcgcttg ctcgtgagct ttcgaatcga ttcgacacat tcgaacaaaa cagacggctc
     3541 gcttgatagc gagagagtca gaggctccag aaggcatcaa aatcaatcat aaaagttcat
     3601 tcccaaccaa gtgggtgtgt ttactgtggc atatcggctc agagctggta ccgctagcgg
     3661 tggcacaggc ataccccaag cgagtgcatc atggccgttt ataagagaga gttgcagcgt
     3721 aagcgttttc ccaaagtgga aagaaccgcc ggcgtcacta attacgaaga gttagccgcc
     3781 agggcgctgg gcaattctat taatcacctc aacaatgctt attcgcagaa gctttccggt
     3841 gccgaagggg ccgttgagtt ccgcgatgaa ggtgtttata cctatgacaa ccagggccgt
     3901 cgttatctcg actgtctggg cggcttcggc attttcaacg tcggtcaccg ccatcctaaa
     3961 gttattaacg ccgttaaggc ccagcttgat caagttgcct tgcactccca ggagctaatt
     4021 aatccctggg cggctcacct ggccagccag ttggctgcag tggcaccggg cgatctgcaa
     4081 tattgcttct ttggcaacag cggatcggag gctgtggaag gggcaatcaa gctggctcgt
     4141 ttgtacaccg gcaaaaccga gattatttcc accaagaatg cctaccacgg tgtcagcatg
     4201 ggggctctct ccgccacggg gcgcgatgtt tttcgcaaac cattcgagcc tctattaaat
     4261 ggtttcgtgc atgtaccctt cggcgatatc gcagcgctgg aagcggccat caatgagaat
     4321 accgcagccg tcattctgga acctattcag ggtgaagggg gcatcaacgt gcctccgacc
//
LOCUS       DAOQNZ010000134         4379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_8813_length_4379_cov_4.197502, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000134 DAOQNZ010000000
VERSION     DAOQNZ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8813_length_4379_cov_4.197502"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /gene="nadD"
                     /locus_tag="PKW73_03525"
     CDS             complement(<1..889)
                     /gene="nadD"
                     /locus_tag="PKW73_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNA72380.1"
                     /translation="MLSPCGFIARSRSFHDSRRVLRTCRTRLLSLRRAYSRPEMVFVC
                     QPGRSVTDGNRSYFMKKRRIGIYGGKFDPPHLGHLLCAEMTREAFALDKVLFVTSAVP
                     PHKDSGVTDARIRHEMVEAACAPNCFFEACDIELKREGKSFTLLTVRQLREIYGPDTE
                     LCLMISSEYLDPDYEWNVGRWTGSDELLKMVRLLVFTRQGHTNELSRAWGALLQEKFA
                     ASFAVQIDYLDFCPSPPTSSTLLRERVSKQLSIWYMVLPEVWDIIRVRGLYGCKTPPR
                     GSDLRKRWRRLTVSAKRYWN"
     gene            complement(1280..1537)
                     /locus_tag="PKW73_03530"
     CDS             complement(1280..1537)
                     /locus_tag="PKW73_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72381.1"
                     /translation="MTRVQKNLIAAIAVCAVILGAIYCYFMQLVREAQNPPGPTERVA
                     DPLGSGDKKAAPPDEPEVDMDKLFEEERKKQKGLGSLRKSD"
     gene            complement(1653..2813)
                     /locus_tag="PKW73_03535"
     CDS             complement(1653..2813)
                     /locus_tag="PKW73_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNA72382.1"
                     /translation="MKSVFLSPHFPPSFANFAGRLKEQGVAVLGLADQPYDDLSPELK
                     RSLTEYYRVSDMHNYEELVRAMGYFTHRYGKIDHIDSHNEYWLETEARLRTDFNIAGI
                     KCGQIENIKCKSKMKEVFRNAGLSPARGRICRSDEELRSFIKEVGLPVVAKPDNGVGA
                     AATFKIEGEADIQRFWTEKAYCDYIVEEFISGDIVTFDGLVDQRGELVFSTSLRYCRG
                     IMEIVNEDSDVYYWTVRSIEPDLEQVGLSTLKAFDVKARFFHLEFFRTGDGKLMPLEV
                     NMRPPGGLTLDMINYVFDFDCYRAWAEMLVHGHNPRVKERPYFVIYVGRKDWIPYVLH
                     HDQVLHQFAPLLKQEERMAGVFARAIGNHGYILRHEELEPLLAAAASILQRA"
     gene            2854..3729
                     /locus_tag="PKW73_03540"
     CDS             2854..3729
                     /locus_tag="PKW73_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNA72383.1"
                     /translation="MHQKNTIRSKDGGSFRRCYNWLPIAALKAAPEAEGKNSIMLREY
                     HKSYSRALERDMEMLIFGHAGARILAFPTSCGRFFDWENFGLVESLGEALSRGWFQLF
                     CLDSVDVESWWNQNINPEARLERHLAYQKYVIEEALPLTESRNSHSYSIAAGCSFGAY
                     HSANLALRFPDRFNRVLGMSGVYEVRTWTDNRMDDQLALGSPSDLISKINDQPLLEQI
                     RSIDWILPTGAEDPLLPSNEDFSQALKAKGVPHHVDIWDGNCHDWLSWSKMLPKYIGG
                     VPAGQTSTAIGLKSL"
     gene            complement(3693..>4379)
                     /locus_tag="PKW73_03545"
     CDS             complement(3693..>4379)
                     /locus_tag="PKW73_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNA72384.1"
                     /translation="DLCRYFSAHIVGSGKLLQDASKKEMQRTQWPVTNSTFGQEYGLG
                     LQIDYVEQRRVFGHAGGFPGHATRTLCDPADKMVVSVLTNCIDADPTAIARAVFAFAD
                     HFSAAYAAGEDSTMVKAAEASGSIDMERFRGRFQGLFRIMDVLPAGDKLIAISPSSWQ
                     PFADVEELSVVNEHTLKMMKANGFSCEGELVPYEFRGAELPADSIRCGGATLFGEKNY
                     KLFNPIAVEV"
BASE COUNT         1088 a         1234 c         1107 g          950 t
ORIGIN      
        1 gattccagta acgcttcgcc gatacagtca agcgacgcca cctcttgcgc aggtctgacc
       61 cacgcggtgg cgtcttgcag ccgtacaggc cgcgaacgcg gatgatatcc cagacttccg
      121 gtaacaccat gtaccagatc gacagctgct tgctgacgcg ctcacgcaga agcgtgctcg
      181 acgtcggcgg gctggggcag aagtccaggt agtcgatttg caccgcaaaa gaagccgcga
      241 acttctcctg cagaagcgct ccccaggcgc gcgacagctc gttggtatgt ccctggcgcg
      301 tgaacaccag aagacgcacc atcttgagca gctcatccga accggtccag cgccccacat
      361 tccattcgta atcggggtcc agatattcgg aagagatcat caggcaaagc tcggtgtccg
      421 gtccgtagat ttctctcagt tgcctgacgg tgagcagagt gaaagacttg ccctcacgct
      481 tgagttcgat gtcgcaagcc tcgaagaaac agttgggtgc acatgccgcc tcaaccatct
      541 cgtggcgaat gcgggcgtcg gtgacaccac tgtccttgtg cggcggcacc gcactggtca
      601 cgaagagaac cttgtcgagg gcgaaagcct ccctggtcat ctctgcgcag agcaggtgcc
      661 ccaggtgcgg agggtcgaac ttacctccgt aaatgccaat tcgtcgtttt ttcatgaaat
      721 agctcctgtt gccgtctgtg actgaacggc ctggttggca gacaaaaacc atctccggtc
      781 ggctataagc cctacgcaga gacagtagac gggtgcgaca agtacgtaga actcgccgtg
      841 aatcgtggaa agaacgtgac cgagcaataa atccgcaggg cgacaacata ccgggactaa
      901 tcctttacgg ttgtcctcgg agaaaatgct tacgacgcgg gaaatttagt tactcgatag
      961 ctcttaccag tgatgaaatt gaaatagacc tcgaccctaa ggtttaacct caagaatcac
     1021 aatccgtctt caggtctgta aacatgaact gttacaggtt tcaggactcg aaagcctgtt
     1081 ttactgttca tgtgaaagat gaaatgaagg aggcattaac tagcgccgtt cgcgctagtt
     1141 gccaagtgtc ccatttttca gcaaatttcc gtccccggca gcggagtgaa attccgccgc
     1201 ctgactccgg ctcgcgaaac ctcatctcat aaattgcaca taaacatggc tgacttgaga
     1261 ccaatcaaga gcgacctatt caatcactct tgcgcaggga accaagtccc ttctgcttct
     1321 tgcgttcctc ttcaaagagc ttatccatat ccacttccgg ctcatcaggc ggcgcagcct
     1381 ttttatcccc agaaccgagc ggatcagcca cccgttccgt tggtccaggc gggttctggg
     1441 cctcccgcac taactgcatg aaataacagt aaattgcgcc gagaatgaca gcgcacacgg
     1501 caatggcggc gatgaggttc ttctgcaccc tggtcatgga caacaccctg cttgaaactg
     1561 aactggcaac caaattcgta gtaaatgccg gcgcgccatt accggcatgg acgccaagca
     1621 taaaccaaag agccatcgct caacaaggcg gcctaagccc tttgcaagat ggaagcggcc
     1681 gcagccagaa gcggctccaa ctcttcgtga cgcaggatat aaccatgatt acctatggcc
     1741 ctggcgaaaa cgccggccat acgctcttcc tgcttgagca agggagcaaa ctgatgcaag
     1801 acctggtcat gatgcaaaac atagggaatc cagtctttgc gtccgacata gatgacgaag
     1861 tacggtcttt ccttcacccg tgggttatgc ccatgcacga gcatttcagc ccaggcccgg
     1921 taacaatcga aatcaaagac atagttgatc atatccaggg tcaggccgcc gggaggtctc
     1981 atgtttacct ccaggggcat gagcttaccg tcgccggtac ggaaaaactc caggtggaaa
     2041 aagcgggctt tcacatcaaa agctttcaaa gtagaaagcc ccacttgctc aagatcaggc
     2101 tcgatggagc gcaccgtcca atagtagaca tcggagtctt cgttgactat ttccatgatg
     2161 ccccggcaat agcgcaaaga ggtagagaaa accaactctc cccgctgatc taccagaccg
     2221 tcgaaagtga caatatcgcc gctgataaat tcttccacga tgtagtcgca ataggccttc
     2281 tcagtccaaa atcgctgtat atcagcctcg ccttcaattt tgaaagtggc ggcggcacca
     2341 acaccattgt ctggttttgc caccaccggc aatcccactt ctttgatgaa actcctcagt
     2401 tcttcgtcac tgcggcagat ccgaccacgg gccgggctaa gaccggcatt gcgaaagact
     2461 tccttcattt tggacttgca cttgatgttt tcaatttgcc cacacttgat gccggcaata
     2521 ttgaaatcgg tgcgcaagcg ggcctcggtc tccaaccagt actcgttgtg cgaatcgata
     2581 tgatcgatct tgccgtaacg gtgagtaaag taccccatgg cccgcaccag ctcttcataa
     2641 ttgtgcatat ctgagacacg gtagtactca gtcagggaac gcttcagttc gggactgaga
     2701 tcgtcataag gctgatcggc cagaccaaga acggctacgc cctgctcttt caatcgaccg
     2761 gcgaaattgg cgaaggaagg cggaaaatgc ggcgacagaa aaacagattt cataacaccc
     2821 tctctttgat gctgaccacc agggctatta tcagtgcacc aaaagaatac catccggtcc
     2881 aaagacgggg gttcctttcg acgatgctat aattggctac ccatcgccgc tttgaaagcg
     2941 gcaccagaag cggaaggcaa gaactcaatt atgttgcggg aatatcacaa aagttacagc
     3001 agggccctgg agcgcgacat ggaaatgctc attttcggtc atgccggagc cagaatttta
     3061 gcctttccca cctcttgcgg gcgcttcttc gactgggaga attttggtct ggtggaaagc
     3121 ctgggcgagg ctctgagccg cggctggttt caactcttct gcctggactc cgtcgacgtt
     3181 gaatcctggt ggaaccagaa tatcaatccg gaagctcgcc tcgagcgcca tcttgcttac
     3241 cagaaatatg tcatcgaaga agctctaccc ctgacggaaa gtcgcaattc acactcctac
     3301 agtatcgccg ccggttgcag tttcggcgcc tatcactcgg ccaatctggc tctgcgtttt
     3361 cccgaccgct tcaatcgtgt attgggtatg agcggcgtct acgaagtgcg cacctggacc
     3421 gacaatcgca tggatgacca gcttgccctg ggcagcccca gcgatctgat ctcaaaaata
     3481 aacgatcagc cgcttctcga acaaatccgt tcaatagact ggattttgcc taccggcgcc
     3541 gaggatccac tattgcccag taatgaagac ttttcacaag ccctgaaagc aaagggcgtg
     3601 ccgcaccacg tcgacatctg ggacggcaat tgccatgatt ggctttcctg gagcaagatg
     3661 ctgcccaagt acatcggcgg cgtcccagcc ggtcaaactt ctacggcaat tggattgaag
     3721 agcttatagt ttttctcgcc aaagagagtg gcgccgccgc aacggatgga gtcggccggc
     3781 agctccgcac ccctgaattc atagggcaca agctctcctt cgcaagaaaa gccgttcgct
     3841 ttcatcattt tgagagtgtg ctcattcacc acgctaagct cttcaacatc ggcaaaaggc
     3901 tgccagcttg agggactgat agcgataagc ttatccccgg caggcaatac gtccatgatg
     3961 cgaaagagac cctggaagcg cccgcgaaac cgctccatat cgatggagcc gcttgcttcg
     4021 gcagctttta ccatagtcga atcttctcca gccgcataag cagccgagaa atgatcggca
     4081 aaggcaaaga cagcgcgggc aatggcagtg ggatcggcat caatacaatt agtcaaaacg
     4141 gaaaccacca tcttgtcggc aggatcgcag agagtgcggg tagcatggcc cgggaagccg
     4201 cccgcatgtc caaagacacg cctttgctcc acatagtcga tttgcagacc aagaccgtat
     4261 tcctgaccga aggtactgtt tgtcacaggc cactgcgtgc gctgcatttc cttcttgctg
     4321 gcatcttgca gaagcttgcc ggaacctact atgtgtgccg agaaatagcg acagagatc
//
LOCUS       DAOQNZ010000135         4305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9054_length_4305_cov_3.468000, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000135 DAOQNZ010000000
VERSION     DAOQNZ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9054_length_4305_cov_3.468000"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PKW73_03550"
     CDS             <1..822
                     /locus_tag="PKW73_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNA72385.1"
                     /translation="AYLTDLDCYLGYFRRQRLSKWGLYHWRNVLESGVDDNLALIAPR
                     EAAKDEDDSIARFDDGRILAVDINSYLAGEFNALARLAEACGNKALQDACAIHASELC
                     QAIEEHLWHEKLALYCGYNTADYTTVALRSWTGLTPVIMGFAEEERAHRVIRENILNQ
                     EHFLRPAGLASHAVSEPLANQQRRGLYGRAIVSNWQGPVWVLPNALIVRALLKYGYKK
                     EAQDIAARVVATLGKGLDETGTLYENYNAETGAPLWAPNFMSWNILALELIELLE"
     gene            complement(960..1736)
                     /locus_tag="PKW73_03555"
     CDS             complement(960..1736)
                     /locus_tag="PKW73_03555"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNA72386.1"
                     /translation="MTTAQVQTQAAPPKNLLGKIAIVTGGSRGIGAAIAARLAAQGAT
                     VFITYARSKESAEKVAAAITLAGGKAIAVQANVASAADNQALVAQVAKHGKIDILVNN
                     AGVFEGGSIEAVSIDQYERVFDTNVRGVAVTTMAALPQMNDGGRIINISSVAARAIAP
                     GFGFYSATKAALDALTRAWAQDLGKRGITVNSVSPGTTLTEMLEAGLPEAAKQTYVSK
                     TALGRLGEPDDIAAVVAFLAGPDGLWMNGKVLEADGGLAV"
     gene            complement(1928..2848)
                     /locus_tag="PKW73_03560"
     CDS             complement(1928..2848)
                     /locus_tag="PKW73_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72387.1"
                     /translation="MKQSRQNLLLLTVAVSFFLLPGAALCQPQKQGAERASLQFAGKA
                     ASKGNSAAASAERQGSEKRAKKLVFPVASLSPVQRDAYNMALKADRCNSIMESEEALA
                     LCNQALKLDPHCWKAYLARGQAQINSNRPDQALSDFSRVIDSKDQYFMYGARRLRADL
                     YCVLKRFAEAIKDIDALEAEGATLSDGMHETRAGCYSGLGKHAQAIADYTAAIALRPN
                     TTRLRFKRAESYLHAGQYEKAVADYTRVIEVDGKDGNFMGGSPEAYLGRASAYDLLGR
                     KDLANKDRAAARNADQQNYADAPFRSPGRK"
     gene            3205..>4305
                     /locus_tag="PKW73_03565"
     CDS             3205..>4305
                     /locus_tag="PKW73_03565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA72388.1"
                     /translation="MPSAIVNAPGRARTRKSRSTLQNLVAVGLDGNSAVRPIPQDSRW
                     QVLTATWLGEMFDGMDASIYVLVMHQCLSELIGASAHSVVAPVGALVLAIFIIGWVIG
                     GISCGILADYFGRSRIMLCTILLYALASGLCAFSHNWQELAFYRFLVGFGIGGEIGIG
                     AVMVAETFKTKSRMHAASFLASSFACGYLLAAVANYYIGHLGWRYLFLLGVLPALVTV
                     YFRTKLKEPEHFEEVKREKRIAKMVARASGNRLKAEGFTLPQVFSQKNLHSTLVAMGL
                     STAAIVGYWAVLAWLPAWVTQLVGGTAVHERSLAAVTMNIGGLSGSLIAGFLVSKLGR
                     ARSFRLANLMTFGSCLLLFGTVKHYGNELLILV"
BASE COUNT          965 a         1178 c         1196 g          966 t
ORIGIN      
        1 gcctatctca ccgacctcga ctgctatttg ggttatttta gaaggcaacg tctgagcaag
       61 tgggggctgt atcactggcg caatgtgctg gaaagcggcg tggacgacaa tctggcattg
      121 attgctccca gagaagcggc caaggatgaa gacgattcca tagcccgctt tgacgacggg
      181 cgaattttag cagttgatat aaatagttat ctggccggcg agtttaacgc tctggcgcgc
      241 ctggcggaag cctgtggtaa taaggctttg caagatgcct gtgcgattca cgcaagtgaa
      301 ttgtgccagg caatagaaga gcacctctgg cacgaaaagc tagccctata ctgcggctat
      361 aataccgccg actataccac tgtcgccctg cgcagctgga caggattgac accggtaatt
      421 atgggttttg ctgaagagga acgggcccac cgcgtaatcc gcgaaaacat cctcaaccag
      481 gaacacttct tgcgcccggc cggactggca tcgcatgcgg tatcagaacc actggccaat
      541 caacaaaggc gcgggcttta cggccgggcc atcgtttcca actggcaggg accggtctgg
      601 gtactaccca atgccctgat agtgcgagcc ctgctcaaat acggttacaa gaaggaagct
      661 caagacattg ccgcccgagt tgtggccacc ctcggcaaag ggctggacga aacaggcacg
      721 ttgtacgaaa actacaatgc cgaaacaggg gcgccactgt gggctcccaa cttcatgagc
      781 tggaacatcc tggcccttga actgatagag ctgctggaat aattcttcct cttttgagca
      841 agcattgaag caagttcaga aagttcagaa aggttcagaa aggttcggaa gggttcagaa
      901 agtttcagcc caaaccagcc tcgagcaggc tggtctgggc gagataactt cacccggggc
      961 tagacagcca gaccgccgtc tgcctccagc accttgccat tcatccagag tccgtccgga
     1021 ccggccagga aggccaccac ggcggcaatg tcgtcgggct caccaagcct gcccagggca
     1081 gtttttgaaa cataagtctg cttggccgct tccggtaacc cggcttccag catctcggtg
     1141 agggtggtgc ccggcgaaac ggagttaacg gttatgcctc tcttgccgag atcttgagcc
     1201 caggcccgtg tcagggcatc gagggcggct ttggtggcgg agtagaatcc gaaaccgggg
     1261 gcaattgctc tggctgccac cgaagagatg ttgatgatcc tgccgccgtc gttcatctgg
     1321 ggaagggcag ccatggtggt cacagccaca cctctaacat tggtatcgaa tacacgctcg
     1381 tactggtcaa tcgatacggc ttcgatggaa ccaccctcaa atacaccggc gttgttgacg
     1441 agaatatcga tcttgccgtg cttggctacc tgagccacaa gagcctgatt atccgcagcg
     1501 gaggcaacgt tggcctggac ggcgatggct ttgccgccgg caagtgtaat tgctgcggcg
     1561 accttttcag cactctcttt gcttctagca taggtgatga aaacggtggc gccctgggca
     1621 gcaagccgag cggcgatggc agcgcctatg ccccgggatc cgccggtgac tatggcgatc
     1681 ttgcccagaa gatttttggg cggggctgcc tgagtttgaa cttgagctgt cgtcatggaa
     1741 ccctccagag ggaacggtag tgggaccgac ctcaaaaggt cgatcgagtt aataacataa
     1801 tttgaaccgc ggcgttaaga acagattaga ccggtcgtct cgctctcgtc aactcaccca
     1861 aaaataaact tacaattgcc tctcttccca gcaaatacag ccgcatctac tacaactgcg
     1921 tagcgattta ctttctacct gggcttcgaa agggcgcgtc ggcgtagttt tgctgatcgg
     1981 cgttgcgggc agcggctcga tctttatttg ccaggtcctt acggccaagc aaatcatagg
     2041 cagaggcacg gcccagataa gcctccgggc tgcctcccat aaaattgccg tccttcccat
     2101 ctacctcaat aaccctggtg tagtcggcca cagccttctc atattgtccg gcatgcaaat
     2161 agctctcagc gcgcttgaac cgcaacctgg tggtattcgg tctcagtgca atggctgcag
     2221 tgtaatcggc gatggcctgg gcatgtttgc ccaaaccgct gtagcagccg gctctggtct
     2281 catgcatacc gtcgctcagg gtggcacctt ccgcctccag agcgtcgata tcctttatgg
     2341 cctccgcaaa acgcttcagc acacagtaaa gatcggcccg caagcgcctg gctccataca
     2401 taaaatactg gtctttactg tcaatgaccc tggaaaagtc ggacagggcc tgatcgggtc
     2461 gattcgaatt tatttgagct tgccctctgg caagatatgc cttccagcag tgaggatcaa
     2521 gcttcaaggc ttgattgcag agagcgaggg cttcctcgct ttccataatg gaattgcacc
     2581 ggtccgcttt gagagccata ttgtaggcat ctctctgcac gggactgagg ctggcgacgg
     2641 ggaaaaccag tttcttcgcc cgtttttctg aaccttgccg ctctgcggaa gccgcggcgg
     2701 aattgccttt agaggcagcc ttgcctgcaa attgcaacga cgcccgctcc gcaccttgct
     2761 tttgcggctg gcaaagggct gccccaggca agagaaagaa tgaaacggca acggtcagga
     2821 gcaaaaggtt ttgacgagac tgcttcaatt tcacctcaga taaaaaacct gccgccttct
     2881 attgtcgcac caggtaaaca tcggtggagc agtgctctat cagataatga gcaatggggt
     2941 catgctcgct ctcagaaccc tcttgcttga aggtgtcgac attgcaggag aggacaacag
     3001 aaaaacaggg ctgtatgggt ttaagagcag tttaggagta agacagtctc tcaaattttg
     3061 gatcccgcac cagaccgcct ctccacttga gcagaaacgt cgcctctcct agtaataacc
     3121 gcagccggtg accacagttc agacacaaga caactctagt atgaggccaa gaaccaaccg
     3181 tctttagata aagaggatac aagcatgccc agtgcaatcg tgaatgcgcc cggtagagcc
     3241 cgcactcgca agtcgcgctc caccttgcaa aatctggttg cagtaggtct cgatgggaac
     3301 agcgccgtcc ggccgattcc gcaagacagt cgctggcaag tgcttacggc cacctggctg
     3361 ggcgaaatgt tcgacggcat ggacgcctct atttatgtgc tggtcatgca tcagtgcctg
     3421 tcggaactta ttggcgccag cgcccatagc gtggtggcac cggtgggggc attggtgctg
     3481 gctattttca tcatcggctg ggtaattggc ggcatcagct gcggtatcct ggccgattat
     3541 ttcggacgtt cccgcatcat gctctgtacc atcctgctct atgcactggc cagcggtctg
     3601 tgtgcattca gtcataactg gcaggagctg gccttctatc gctttctcgt gggcttcggc
     3661 ataggcggtg aaatcggcat cggcgccgtc atggtggcag agactttcaa gacaaaatcg
     3721 cgcatgcatg cagcgtcctt tctggccagc tcctttgcct gcggctactt gctggccgct
     3781 gtagctaatt actatatcgg ccaccttggt tggcgctatc tctttttgct gggcgtcttg
     3841 ccggccctgg tcacggttta cttccgcacc aagttgaaag aaccggagca tttcgaggaa
     3901 gtgaagcgag aaaagcgcat tgccaaaatg gtagcgagag caagcggtaa tagactgaag
     3961 gctgagggct tcaccctgcc gcaagtattc tcgcaaaaga acctgcatag cacccttgtg
     4021 gccatgggtc tttccacagc ggccatcgtc ggttactggg ccgtgctggc ctggctgccg
     4081 gcctgggtaa cacagcttgt gggcggaacg gcagtgcacg agcgcagtct ggctgccgtc
     4141 accatgaaca taggcggttt gagtggttct ctcatagccg gctttctggt gagcaagtta
     4201 ggcagagcga gaagcttccg cctggccaac ctgatgactt tcggcagttg cctgctgctt
     4261 tttggcaccg tcaaacacta tggaaacgag cttctcatac tggtt
//
LOCUS       DAOQNZ010000136         4303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9060_length_4303_cov_4.010829, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000136 DAOQNZ010000000
VERSION     DAOQNZ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9060_length_4303_cov_4.010829"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKW73_03570"
     CDS             complement(<1..226)
                     /locus_tag="PKW73_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72389.1"
                     /translation="MSLFDRILHKWLFRSLANQLLITYLLVITIALMAVSLWALVAIR
                     KESLTDLQNTLEVEAVNLGLEIDNDLVLDSP"
     gene            659..979
                     /gene="trxA"
                     /locus_tag="PKW73_03575"
     CDS             659..979
                     /gene="trxA"
                     /locus_tag="PKW73_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNA72390.1"
                     /translation="MSVANVTDSTFEQEVLKADIPVLVDFWAPWCGPCKAVAPVVEDL
                     SKEYEGRLKVVKLNTDENPKTAQAYKIRGIPSLFLFKSGQVMEEVVGAVPKSTLTNHI
                     NKHV"
     gene            1056..1601
                     /locus_tag="PKW73_03580"
     CDS             1056..1601
                     /locus_tag="PKW73_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNA72391.1"
                     /translation="MQLNQWSRRSFALSAALLVGFGLVASTSQEASARHHRRHHRVVR
                     HNDLASVAARTPELKTTMEAYKKAGMVGALRAGGQRTVFMPSDAGWGKMAKENREGLM
                     NDPKKLQEVLKYHHLKGKQEAAELLNKRSAVTLQGESLMLDNKDGTLIVDGCVTTTPD
                     VKASNGVIHIIDYIPIPERGK"
     gene            1896..2366
                     /locus_tag="PKW73_03585"
     CDS             1896..2366
                     /locus_tag="PKW73_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNA72392.1"
                     /translation="MKELIIGFLIALVISSYMNGSNIDSQFGWNQAAASLPGAGGNQG
                     SELIDDINEGTFQGEVLDSHEPVLVEFYSQDCVHCTNMKPELGKLAQESQGYLKVYKV
                     DAKANGALAERYEVMALPGFVLFSKGKPVNSTSGEMSKQELSNWVKKELDIPVS"
     gene            2417..2665
                     /locus_tag="PKW73_03590"
     CDS             2417..2665
                     /locus_tag="PKW73_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72393.1"
                     /translation="MKLTIIGILIALALTTMFGGANYPPVNVPVGVSDVRTSALGTKR
                     QEGGIPELKITDSPRLYRIPEKVTDRHGNRNIDFPTNN"
     gene            2681..>4303
                     /locus_tag="PKW73_03595"
     CDS             2681..>4303
                     /locus_tag="PKW73_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA72394.1"
                     /translation="MKNWARWTLFVLWIVLLVLTAQFWNPFVSFPALLKYSVVSVVAA
                     GVYGALFILIQFYILLSIIFPLPPRADSKAPKSIWRKAFKFLFKESPISPKAPKTLDE
                     MVGNEAAKIEIKEAIDILKKSAHYEGSGAQVPKGMLFVGSPGVGKTLFARAIANEVGV
                     PFYVVEGGAISGLIMGLGVLKLKTLFRKLQRHDRAILFIDEIESMASKRQQDRGFGGM
                     ADMNMTLNTLLTEMDGFHGSRLMVIGATNNDGMLDPALMRAGRMDRRIYFQMPTPQER
                     KDLFKYYLGKVQCDESDLDIDHLTNLTANYSPAEIAGVINEAALIARRPGHPGKVNTE
                     MVLKALDRVSVGLERSITNSDVALISHDPTIRFDSVIGIDDVKQEVTEIVDFLQRGDD
                     LRKIGAKLPKGILMIGPPGVGKSMLAKAIANEAGVPFYGLSASFLIGSVKGEGSNRIR
                     SIYTQARKSPAAIVFIDEIDSIAGTVTDIGSSRTDELNQILVELDGIGRSNVVTIGAT
                     NMEEKLDPAFFRSGRFDRKVYVGLPDKEARALI"
BASE COUNT         1138 a         1196 c         1044 g          925 t
ORIGIN      
        1 ggggggagtc gaggaccaga tcattatcga tctcaagacc caggttgact gcttccactt
       61 ccagggtatt ctgcaagtcc gtcagggact ccttgcgaat cgccaccaga gcccacaggc
      121 tcaccgccat cagggctatg gtaatgacca agaggtaagt tatcagaagc tggttggcca
      181 gacttcggaa taaccacttg tgcagtatgc gatcgaaaag actcacaaaa gacccttata
      241 taacgatatg cataaactaa aggttctcac aaagtaaggg aatacttcca acggcgccac
      301 cacttgaaat gcccctctaa agtctaacct tgtttttaca agtgcccgga acaatcctca
      361 taaagcgcac ccgtcctggc ttccctattc ttacagagtg cttccgccgg cttcttgact
      421 tgcttcaaaa tcgccactgt gagatgtggg ctgtgggaaa cttgttcaca atcactataa
      481 tctctttgta tgtttgactg agctattggg aaattgccga ctatcgcagt cggatttatc
      541 cacggtgatg cctaaagcaa ggcaaaactc ttagtacccg gtaaagcggt caaattaaga
      601 gagcacaaat tgaaagcatt cagcaagtgc tgagccacat gggaggtcct gtttagaaat
      661 gtccgttgca aacgtaaccg attccacctt cgagcaggaa gtcttgaaag ccgacattcc
      721 agtgctcgta gacttttggg caccctggtg cggaccttgt aaggccgtcg cgccggtggt
      781 cgaggacctt tccaaggagt atgagggaag actgaaagta gtcaaactca ataccgacga
      841 aaatcccaaa accgcgcaag cctacaaaat ccgcggcatt cccagcttgt tccttttcaa
      901 aagcggacag gtcatggaag aagtggttgg agctgtgccc aagtccacgc tcacaaacca
      961 cataaacaag cacgtttaat tatcaagcag cctagacaag tagtcagtaa agccggcaag
     1021 ggtgtccccc ccggctgaat tgaaaggaga agctaatgca acttaatcag tggagtcgcc
     1081 gttcattcgc gctttctgcc gcgcttttgg tcggtttcgg tctcgtagcg agcacttcgc
     1141 aagaagcctc ggccagacac cacagaagac atcacagagt agttcgtcat aacgacctgg
     1201 cctctgtcgc cgctcgtacc cccgaactca agaccaccat ggaagcctac aaaaaagccg
     1261 gcatggtcgg agctctgaga gccggcggtc agcgcaccgt cttcatgccc agcgacgccg
     1321 gctggggcaa aatggctaaa gagaaccgtg aaggtctgat gaacgatccc aagaagctac
     1381 aggaagttct caagtaccac cacctgaaag gcaagcaaga agcggctgaa ctcctcaaca
     1441 agcgctccgc cgtaactctt cagggcgaaa gcttgatgct cgataacaaa gacggcactt
     1501 tgatcgttga tggttgcgtt accacaaccc ccgacgtgaa agcctccaac ggcgttatcc
     1561 acatcatcga ctacattccg attcctgaaa gaggcaagta agagccgccc ggggcttaac
     1621 ccatttagaa tcgaagcagt tgcaaggaaa cggacgagaa atcgtccgtt ttcgctatat
     1681 tcagggtaat tacccgttgt gaaaccgtaa tcggtatgtg taaatctcat aaggctgtgc
     1741 caatatatat gtagtctggt acacaggtgc aaacccggct cagacctttt gcccggcaaa
     1801 gccgaggcac ataactttcc aagcaaactc tgtttaaatg tccatagata gaccgctcac
     1861 cttcgcccgg tacccttgaa tcaaaaggtc agtcaatgaa agaactcatc ataggttttc
     1921 tcatagctct ggtaataagc agctacatga acggcagcaa tatagacagc cagttcggct
     1981 ggaatcaggc ggcggctagt cttcccggtg caggcggcaa tcaaggcagc gagctaatcg
     2041 atgacatcaa cgaaggcacc ttccaggggg aagtgctgga tagccatgag ccggtgctgg
     2101 tggaattcta ctcacaagac tgcgtgcatt gcaccaatat gaaaccagaa ctgggcaaac
     2161 tagcccagga aagccagggc tacctgaaag tctataaagt cgacgccaaa gccaacggcg
     2221 ccctggcaga gcgctacgaa gtcatggcct tgccgggttt tgtgctcttc agcaaaggta
     2281 aaccggtcaa ttccacttcc ggagagatga gcaagcaaga gctttccaac tgggttaaaa
     2341 aggaactgga cataccggta agctaggcga accggtaagc agaacactgc atacatacgc
     2401 aaacgcggat cactgaatga aactgacaat aataggaata ctaatcgccc tggccctcac
     2461 cactatgttc ggaggcgcca actatccgcc tgtcaacgtt ccagtcggcg tttccgatgt
     2521 gcgcacttcc gccctgggta ccaagagaca ggaagggggc attcccgaac tgaaaatcac
     2581 cgattcgccc aggctgtacc gcattcccga gaaggtaacc gaccggcacg gcaatcgcaa
     2641 tatcgatttt ccgactaaca actaggagac gacctgaatc atgaaaaact gggctcgctg
     2701 gacacttttc gttctatgga ttgtacttct ggtcttaacg gcgcagttct ggaatccctt
     2761 tgtctccttc ccggctctgc tcaagtactc cgtcgtctcc gtagtggcag ccggtgttta
     2821 cggcgccctc ttcattctca tccagttcta catcctgctt tccattatct tcccgctacc
     2881 gccccgggct gattccaaag ctcccaagag catctggcgc aaagccttca aattcctctt
     2941 caaggaatcc cccataagcc ccaaagcccc caagaccctc gatgagatgg tgggtaacga
     3001 ggccgctaag attgaaatca aagaagccat cgatattctc aagaaatccg cccactatga
     3061 aggctccggc gctcaggtgc ctaagggtat gctcttcgtg ggttcccccg gcgtgggcaa
     3121 aaccctcttt gccagagcca ttgccaatga agtgggtgtg cctttctatg tagtagaagg
     3181 cggtgccatc agcggtttga tcatgggtct gggagtgctc aaactaaaaa ctctcttccg
     3241 taaactgcaa agacacgatc gcgccatcct cttcatcgac gaaatcgaat ccatggcctc
     3301 aaagcggcag caagaccggg gcttcggcgg catggccgac atgaacatga ccctcaacac
     3361 cctgcttacg gaaatggacg gcttccacgg ctctcgcctg atggtcattg gtgccaccaa
     3421 taatgatggc atgctcgatc ccgccttaat gcgggcaggc cgcatggaca gacgcattta
     3481 cttccagatg cccactccgc aagagcgcaa agatctcttc aagtactacc tgggcaaagt
     3541 gcaatgcgac gaaagcgatc tagatattga ccatctaaca aacctgacgg ccaactactc
     3601 acccgctgaa atcgccggtg tcatcaacga agccgctctc atcgccagac gtcccggcca
     3661 cccaggcaaa gtcaacaccg aaatggtttt aaaagccctc gatagagtct cggtcggcct
     3721 tgagcgctcc attacaaact ccgatgtcgc tctcatcagc cacgacccca ccattcgctt
     3781 cgactcggtg ataggtatcg acgacgtcaa gcaagaagtg acggaaatcg tcgacttcct
     3841 gcaaaggggc gatgacctgc gcaagatagg agccaagctg cccaaaggta tcttgatgat
     3901 cggccctcct ggcgtgggta agtcgatgct ggccaaagcc atcgccaacg aagctggtgt
     3961 gcctttctac ggtctttctg cttcattctt gatcggctcc gttaaaggcg agggctccaa
     4021 tcgcattcgc tccatttaca cccaggcaag aaagagcccg gcggccattg tctttatcga
     4081 tgaaatcgat tccatcgccg gcaccgtcac cgacataggc tcctcaagaa ccgatgaact
     4141 caatcagatt ttggttgaat tggacggcat cggccgctcc aacgttgtca ccatcggcgc
     4201 caccaacatg gaagagaaat tagacccggc cttctttcgc tccggtcgtt tcgatagaaa
     4261 agtctatgtg ggcttgcctg ataaagaggc cagggccctc atc
//
LOCUS       DAOQNZ010000137         4302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9065_length_4302_cov_3.967977, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000137 DAOQNZ010000000
VERSION     DAOQNZ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9065_length_4302_cov_3.967977"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1503
                     /locus_tag="PKW73_03600"
     CDS             283..1503
                     /locus_tag="PKW73_03600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNA72395.1"
                     /translation="MVAALLLYCDFKEQAMLASSDYLMTFHTAGWIAGHGQWDILYPQ
                     PGAGTFHGEPFDVKAHELLPFLPDYSVAEYMYMPASAFLFLPFAELPPNLSLFAFQLL
                     SLACLFGSSLLIPAGTKMGRAFTQGLSLLTFLPTFLTLWIGQVGLVFGLFPLSCGYYL
                     LSKKQDFPAGLVFSLLALKPQMLVPAIFLLFLKLCQKQFSLAFGMVAGLIGLAFLNYA
                     LLGQKLLLSWFDCLKLSDRIYSDPAMGVPVRLATSLPRAILLSQPEQVRLTLKPYLYA
                     GAVLLACLGLALVFVLARKASPMSEGDKLKSALILGCLALPCVVPHLFIYDLCVLAPA
                     GFLVFAPVAAEGETFACLRPFLLMLWLSVTAYCMLLMSCPGVVSPLVLCGIILLVYVG
                     AMLRLYAGRLKVRS"
     gene            1508..3031
                     /locus_tag="PKW73_03605"
     CDS             1508..3031
                     /locus_tag="PKW73_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72396.1"
                     /translation="MAKKISRRFDRLVIWPLCWLSLGALVFFYFKGFEAALTAPLSLG
                     GEGPSLYAAYMLSQNLNPYDTLALSEHPWRVVASPPVYFAITGWLLKLSGPSLIPLRV
                     VSAAGFVIFLVAAHRVFALSGASRLGRLLGLINLGAFWSIWAYAFRATPDMLALALMV
                     MAIEQYMVLARKPKDDNIFRFPRLIVIATLAVLASLARGSCAAVVPAIALALIVGRQW
                     RLSLVFMLLSSVLAFASMLLLNNITAGGLAAHLAFSESSPFSFHALHEHLTWLSSDWL
                     ILAAAPVCIINLLVGYINGYEEREGPYRHHLAALVVMVTLFFLSSAVSLYVMGKAAAG
                     VADFFLILFAVAWMVAVSAEHLNRRYLLLMFLAFGCGFFVISSLHVNQNKQIAVMKQG
                     LDEIKERNLNGDLMLCEESSLPQLLDLTPEFVDLPTMRAVWERNPQVYAARMAEIKER
                     IARKGYGSIVINSQDGCLIKPYQYWDEAVVRLIKANYKSVAEIAVDGRMQDFYLPRK"
     gene            complement(3057..4187)
                     /locus_tag="PKW73_03610"
     CDS             complement(3057..4187)
                     /locus_tag="PKW73_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HNA72397.1"
                     /translation="MKKSILLALSLLIISGTNTAASAQNKAAEALALYKQKQYSAAAD
                     AFEKLLKTEKPAARLYYYAALANRDADRKARAVQIFNYIVSNFPTTEEALHSGTMLNN
                     LTGGAKLNSAEKTAAVGEGQPGTGAGSRVNLGSGTGLYPFTPEDIAREGANGIDQSNF
                     ENCWFQASMSALAQLPRGQRLLSTMIRRGEGASYIVRFPGDGNEYVVSRKEARLAGIT
                     DTAVWASLLEYAQTKKFPDNKGAEGTDGDQSRLEVGLGCITGRKAEFLDPGHASASEL
                     ASFIGGAVRSQNPIVCGTYPASMLDKYPPLVIDQHAYTIIGFDPAHNLITIRNPHGRG
                     SRRFSLKSDPQHLKFEQLEDGCFKMHVELFQQYFYSVARSFI"
BASE COUNT          747 a         1151 c         1161 g         1243 t
ORIGIN      
        1 tgcagctttc tctttttctt gctcgaaact gggcatggct atcctctact ggggtgctgg
       61 aagaagattc tcaaagactt ttgcaaactg atttaagtct ggctttcacc aacactaggg
      121 ggcaaatgtt tcaaagtggt tgcttggtgc ctggccttgt ctgcctttag cccttgccgg
      181 gtggtgtatc atgggtgaaa gttccccacc ccggcttgac cagcgttgaa cccaccgcct
      241 cttagcgttt tttttaaaca gatgctctgg ggctggggct taatggtggc agcgctcttg
      301 ctttattgtg atttcaaaga gcaggctatg ctggccagtt ccgattatct gatgactttt
      361 cacacagccg gatggattgc cggacatggg cagtgggata ttctctatcc gcaacctggt
      421 gccggtacct ttcatggtga gccctttgat gtaaaagcac atgagctttt gccctttttg
      481 ccggattatt ccgtggccga atatatgtat atgccggctt ccgcctttct ctttcttcct
      541 tttgcggagc tgccgcccaa tctctctctc tttgcttttc aattgctttc tcttgcttgc
      601 ctgttcggct catctctgct cattccagcc ggtactaaaa tgggccgggc tttcactcag
      661 ggcctttcgc ttctaacttt ccttcctact tttctcaccc tctggattgg tcaagttggt
      721 ctggtattcg gcttgttccc tctctcttgc ggctattact tgctctccaa aaaacaagat
      781 tttccagccg ggttggtttt cagtctcctg gccctgaagc cgcagatgct cgtgccggca
      841 atttttctgc tcttcctgaa gctatgccag aagcagtttt ccctggcttt tggcatggtt
      901 gccggtctca tcggtcttgc tttcctaaat tatgccttgc tggggcagaa gcttcttttg
      961 agctggtttg attgtctcaa gcttagcgat cgcatttatt ccgatcccgc catgggggtg
     1021 ccggtgcgcc tggctacctc cctgccccgc gccattttgc tttctcagcc tgagcaagtg
     1081 cgcctcactc tcaagcccta tctttatgcc ggtgctgttc tgttggcttg cctgggtctt
     1141 gctctcgttt ttgtattggc tcgcaaagca agccccatga gtgagggcga caaactcaag
     1201 agcgctctca tcctgggctg cctggcgctg ccttgcgtcg ttccccatct cttcatttat
     1261 gacctttgtg tgctggcgcc ggcaggtttt ctggttttcg cgccggtggc ggcggaagga
     1321 gagacctttg cctgcttgag accttttttg ctaatgctct ggctatccgt taccgcttat
     1381 tgcatgctct tgatgtcttg cccgggtgtg gtatcgcccc tggtattgtg cggcataatc
     1441 cttttggtat atgtcggtgc catgctcagg ctttatgccg gtcggctcaa ggttcggagt
     1501 tgaagcaatg gccaagaaga taagccgaag atttgatcgc ctggttatct ggcccctttg
     1561 ctggctctcc ctgggcgccc ttgtcttctt ctattttaag ggctttgagg ccgccctgac
     1621 ggcgccgctt tctctcgggg gcgagggacc atccctttat gcggcttata tgctcagcca
     1681 gaacctcaac ccttacgata ccctggccct cagcgagcat ccctggcggg tggtggccag
     1741 tccgcctgtt tactttgcca tcaccggctg gctcttgaag ctgagcggtc cttctttgat
     1801 tccactgcgg gtggtatcag cggccggctt tgttatcttt cttgtggcgg ctcatcgagt
     1861 ttttgccctc agtggcgcct ctcggctggg gcgcctgctg gggctcatca atctgggtgc
     1921 cttctggtcc atctgggcct atgcctttcg tgccactccc gacatgcttg ctcttgctct
     1981 catggtcatg gctatcgagc agtacatggt cctggctcgt aaaccaaagg acgacaatat
     2041 ctttcgcttt ccgcgcttga tcgtgattgc cactctggcg gtgctcgctt ccctggccag
     2101 aggttcttgt gcggcggtgg tgccggccat tgccctcgct ctgatagtcg gcaggcagtg
     2161 gcggctctcc cttgtcttta tgctgctttc atcggtgttg gctttcgcct ccatgctcct
     2221 gctcaacaat attacagccg gtggtttggc cgctcatctg gctttctccg agtcgtcacc
     2281 ctttagcttt catgccctgc acgagcatct gacctggctc agttccgact ggctgattct
     2341 ggcggccgca ccggtatgca taatcaactt gcttgttggt tacataaacg gctacgagga
     2401 acgagagggt ccttaccgcc atcatctggc cgctctggtc gttatggtca ccctcttctt
     2461 tctaagcagc gccgtcagtc tttatgttat gggcaaagct gcggccggtg tggcggactt
     2521 cttcttgatt ctctttgccg tggcctggat ggtggccgtc tctgccgagc acttaaatcg
     2581 gcgctatcta ctcttgatgt ttctcgcttt cggctgcgga ttttttgtta tcagttctct
     2641 tcatgtcaat caaaataagc aaattgccgt tatgaagcag ggtctggatg agatcaaaga
     2701 gagaaatctg aacggcgacc tgatgctttg cgaagaaagc agcttgccgc aattgcttga
     2761 tctgactcct gagtttgtcg atcttcccac catgagggcg gtctgggagc gcaatccgca
     2821 ggtttacgcg gcgcgtatgg ctgagataaa agagcgcatt gccaggaaag gttatggctc
     2881 aatcgtgatt aattctcagg acggctgttt gattaaaccc tatcagtatt gggatgaggc
     2941 ggtggtgcgg ctcatcaaag ccaactataa gagcgtagct gaaatagccg tagatggtcg
     3001 tatgcaagac ttttacctgc cgcgaaaata aacagccgct ataggctgcg cataactcaa
     3061 atgaaagagc gggccacgga gtaaaaatac tgctgaaaaa gttccacatg catcttgaag
     3121 cagccgtcct ccaactgttc gaatttcagg tgctgggggt cgctcttcag gctgaagcga
     3181 cgggagcctc tgccgtgagg atttcggatc gtaattagat tatgggcggg gtcgaagccg
     3241 atgatggtgt atgcgtgctg gtctattacc aggggcgggt atttatcgag catggaagcc
     3301 ggataagtac cacaaactat cgggttctgg gatctcaccg ctccacctat gaaggaagcc
     3361 agttcgctcg ctgaagcgtg accgggatcg aggaactcgg ccttgcgtcc ggtgatgcag
     3421 cccagcccta cttccagccg cgactgatcg ccgtcggtgc cctccgctcc tttgttgtcg
     3481 ggaaacttct tggtctgggc gtattctaag agggaggccc agacagctgt gtcggtgata
     3541 ccggcgagtc tggcttcttt gcgagaaact acatattcat tgccgtcgcc gggaaagcgc
     3601 acgatataac tcgcgccctc gccgcgcctg atcatcgtgg ataagagccg ctgtcctcgc
     3661 ggtaattggg ctagagccga catagaggcc tggaaccagc agttttcaaa gttgctctgg
     3721 tcgatgccgt tggcgccttc tctggctatg tcttccggcg tgaaagggta aagaccggta
     3781 ccggaaccaa gattgacacg actgccggcg ccggtgcccg gctgtccctc gcccactgct
     3841 gccgtctttt cagcgctgtt tagctttgct ccaccggtga ggttgttgag cattgtcccc
     3901 gagtggagcg cttcttccgt ggtggggaaa ttgctcacga tgtaattgaa aatttgtacg
     3961 gctctagcct tgcggtcggc atctcgattg gccagggcgg catagtagta aagccgggct
     4021 gccggttttt ccgtcttcag gagcttttca aaggcgtcgg cggcggcact gtattgcttc
     4081 tgtttataga gagcgagggc ttccgctgcc ttgttctgcg ccgatgcggc tgtgtttgtg
     4141 ccactaatga ttagcaggct aagggccagc aaaattgact ttttcaccgt cagttttcct
     4201 ttctcgataa agtacttcgc gttgccagaa atatgttggc gcagagaacc gccagcaaag
     4261 tgttggtaag caagaaggct attccctctg cttccatgga gc
//
LOCUS       DAOQNZ010000138         4271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9198_length_4271_cov_3.618121, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000138 DAOQNZ010000000
VERSION     DAOQNZ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9198_length_4271_cov_3.618121"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKW73_03615"
     CDS             complement(<1..503)
                     /locus_tag="PKW73_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA72398.1"
                     /translation="MSSENKLDASTAPLPVIKVLMIDDDPYYCRFTKELLSRQENCLF
                     EISTVHTMDEATAVIELEPPHIVVLDLNLRETQGLSTLDKLGDLAISCPILVLTGIDS
                     ESLGLEALSRGAQDYLVKQFITKDSLVRSIRYAVERHKSEEQRLRMAAIQDFTAMLAH
                     DLKVPVLG"
     gene            complement(614..3688)
                     /locus_tag="PKW73_03620"
     CDS             complement(614..3688)
                     /locus_tag="PKW73_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA72399.1"
                     /translation="MTEEAIKQVIDKEIESKSQKLFHEMLGAVHARTDHLFANLLICE
                     WIAGLLVALVVSPRTWYGAYSDVHIHVYTAILLGGLILARPIYLALKRPGRLSTRNSI
                     AIGQMLWAALLIHLTGGRIESHFLIFGSLAFLSFYRDRSVLVYASAVVVIDHIARGIF
                     FPMSIYGVNIIEPWRWIEHAWWVIFEVYFLFKLINQNLDEMREISRKQAELTETRESI
                     ERVVELKTRQLKESQMRASTDYRVAAVLSNAKNLGEAAAELMYALISGLTMSDHYSIV
                     LKEYETEGRFTKTRPVDSSEENDTHRKPLRFFCHSNREQPSGAIDDLMLCLQLPIESN
                     MRSSILGSIEIYTEAGGSIDEADQQMLKAVAGQLADFLDKRATEAENEHLAYLVQSSG
                     DAIVGVNLDGTLSSWNNAAEILVGYQSEHLIGFPYLSLIPEGRRQEQEELFRDATAGI
                     LKKPVDTEILNGQGKCIDVSITMAPVMDENHQILGCSVILRDITDRKLAEKRVAEFYS
                     IVSHELRTPLTSIRAVLGLIEGGIVEPGSGEAQELVQVARASADRLIRLINDMLDLKK
                     IEAGKMDMDFEPTSTRELVESCLQALTGMAEEAEVSVSAEHSFERLVRADRDKATQVL
                     TNLVANGIKFSPPGQSVLVKVSKGETPAKTGEATVRFSVYDRGPGIAEADKVKLFEKF
                     QQLDASDSREKGGTGLGLAISKAIVEQHKGSIGVDSSPGQGSCFWFELPLAQDSNTTA
                     SAPPAKTDLPVLPRSVLLVEDDQGLSTVLTHCLTNAGYRVEQAFNLAEARICLKNSPS
                     VILLDVRLPDGNGLSLIEEIKAEGKTKDIPIIVISGEEAPPDGYGDHAGNNGQTDSGK
                     RHSRPTIQKGSARVLDWLRKPFTEEELTRAVVRATKPSAGKRILVVDDDPGLCKVIET
                     QLVSLGMACVLANDGLTALRLAKDEQPNLIILDVNLPQLNAFEILGALKEGERKATPL
                     IIYTGQDLSREQKQELTLGLTRHLSKRSAGQEDLLQAVQTLLSDIELAPGQN"
     gene            complement(3685..>4271)
                     /locus_tag="PKW73_03625"
     CDS             complement(3685..>4271)
                     /locus_tag="PKW73_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72400.1"
                     /translation="LEKPLDKQILLVFLHPKCPCSAATISELARLVPRLRETQVIAVL
                     TIPAGAPPEFANTEALDRLRSIPGLAIITDTNAQETRNFKAVTSGEVLLYDKSGHLRF
                     CGGITPGRGHQGDSAGKDMLEIISSVNSGKKSTGVEIQPGVKATAEGTYVCPIFGCPL
                     LHDSEQLSETSALSDRANKTIPPGKSIRELSFTK"
BASE COUNT          914 a         1095 c         1095 g         1167 t
ORIGIN      
        1 cccaagactg gaacttttaa atcgtgggcg agcatggctg taaagtcttg aatggctgcc
       61 attctcaatc gctgttcttc ggatttgtgt ctctctacgg cgtagcggat acttctcacc
      121 agggagtctt tggtgatgaa ttgtttgacc agataatcct gggcgccgcg ggaaagggct
      181 tcaagaccca gactctcact gtcgattccg gttaaaacta gaattggaca gctaattgcc
      241 aggtcgccga gcttatcaag ggtgctcagt ccctgggttt cacgtaagtt tagatccagt
      301 accactatat gcggtggctc aagttcaatc acagctgtgg cttcatccat ggtatggact
      361 gtgctgatct cgaagaggca attttcttgg cgcgacagca gttccttagt gaagcggcag
      421 taataagggt cgtcgtcaat catgagcacc ttaattacag gcaacggtgc tgtcgatgca
      481 tcaagtttat tctcggacga catggcgtga tacctcggct ttcgtcaagc tctatcttaa
      541 gtattaccca tctttattca agtgatataa atatcggcct tggattcttt cgataaacga
      601 aggacattga gaattaattt tgcccggggg ctagctcaat atccgaaaga agtgtctgta
      661 cggcctgtag caaatcttcc tgtccggcac ttcgtttact caaatgcctg gttaaaccca
      721 gggtgagttc ttgcttctgt tcccgactca agtcctgtcc agtgtaaata attagcggcg
      781 tagcttttcg ttcgccctct ttgagggcgc caagaatttc aaaggcattc aactggggaa
      841 ggttgacgtc gagaatgatt aaattgggtt gttcatcttt ggccaggcgc aaggcggtca
      901 gaccgtcgtt agctaatacg caggccatgc ccagtgatac aagttgggtc tcaatcactt
      961 tacagagccc gggatcgtca tccacaacaa ggatgcgctt gccggcggag ggttttgtag
     1021 cacgcaccac cgcccgggtc agctcttctt cagtgaaggg ttttctcagc cagtccagaa
     1081 ctcttgctga tcctttttga atggtcggtc gggaatgtct cttgccggag tctgtctgtc
     1141 cgttgttgcc tgcgtggtct ccatatccat cagggggggc ttcttcgccg gaaatgacga
     1201 tgatggggat atctttcgtt ttgccttcag ccttgatttc ttcgatcaga ctcagtccgt
     1261 tgccgtccgg tagtcttaca tcgagcagaa tgacgctggg cgaatttttc agacaaattc
     1321 tagcttcagc caggttgaag gcctgctcaa ctctgtagcc ggcatttgtt aaacaatggg
     1381 tcaagaccgt ggataggccc tggtcatctt ccaccagcaa tacagatctt ggcaggacag
     1441 gcagatcggt tttggccggc ggtgccgacg cggttgtatt actgtcttgt gctaacggta
     1501 actcgaacca gaagcaactg ccctggccag ggctgctatc tactccgatt gagcctttgt
     1561 gctgttctac gatggcttta gatatggcca gtccaagtcc ggttccacct ttctctcggc
     1621 tatcagaggc atccagttgc tgaaatttct cgaagagctt aactttgtcg gcctcggcga
     1681 tacccggacc cctgtcgtag acggagaagc gtacggttgc ttctcctgtc ttggccggtg
     1741 tctctccctt gcttaccttt accaaaacag attggcccgg aggtgagaat ttgattccgt
     1801 tggccacaag gttggtaaga acttgtgtgg ctttatcccg gtctgccctg accagtctct
     1861 caaaggagtg ttcggcgctg acggatactt ctgcttcttc cgccatgccg gtcagagctt
     1921 gcaggcagct ttccaccagc tccctggtgc ttgtgggctc aaagtccatg tccattttgc
     1981 cagcttcaat cttcttcagg tcaagcatat cgttaatgag gcgaatcagc cggtctgcgc
     2041 tagcccttgc tacctgcacc agttcttgcg cttcgcctga acccggctcg actataccac
     2101 cttcaatcag ccccagaacg gctcttattg atgtgagagg agttctcagc tcatgggaga
     2161 cgatggagta aaactccgcc actctctttt ctgccagctt tctgtcggtt atatctctca
     2221 aaattaccga gcatcccagt atttgatggt tctcgtccat cacgggcgcc atggttatgg
     2281 aaacgtcgat gcacttgccc tggccgttca gaatttcagt gtctaccggc ttcttcaaga
     2341 taccggcggt ggcgtccctg aatagttctt cctgctcttg ccttctgcct tcggggatca
     2401 ggctcaggta aggaaaacca atcaggtgct cagactgata gcccaccaga atttcggcgg
     2461 cattgttcca tgaactcaaa gtgccgtcca ggttgacgcc cactatggca tctccactgg
     2521 attgaaccag gtaggctaga tgttcgttct ccgcctcagt ggcacgcttg tccaggaagt
     2581 ccgccagttg gccggcaacg gccttgagca tttgctggtc tgcctcgtcg atggagcccc
     2641 cggcctctgt gtatatttct atacttccca ggatgctact tctcatgttt gattcgatgg
     2701 gaagctggag gcaaagcatt aagtcatcaa tggcgccgga tggctgctcc ctgttgctgt
     2761 ggcagaaaaa acgcaagggt tttctatggg tgtcgttttc ttccgatgaa tcgacgggcc
     2821 tcgttttggt aaagcgaccc tctgtttcgt attctttcaa gactatcgag tagtgatcac
     2881 tcatggtcag accgctgata agtgcgtaca taagctctgc tgcagcttcg cccaggttct
     2941 ttgcgttaga taaaactgct gccacacggt agtctgtgga agctcgcatc tggctttcct
     3001 tcaattgccg ggtcttcagt tcaacgaccc tctcaatact ttcgcgagtt tcggtcagtt
     3061 cagcctgctt gcggcttatc tcgcgcattt cgtcaagatt ttggtttatc aatttgaaca
     3121 ggaagtatac ttcgaagatg acccaccagg catgctcaat ccagcgccag ggctctatga
     3181 tgttgacgcc gtaaattgac atggggaaaa aaatgcctct ggcgatgtgg tcgatcacca
     3241 ccacggctga agcgtagact agaaccgatc tgtcacggta gaaactcaaa aaagccagtg
     3301 atccgaagat aaggaaatga ctttcaatgc gaccgccggt taaatgaatc aagagcgcgg
     3361 cccagagcat ctgtccaata gcgattgaat ttcttgtgct cagccggcct ggccgtttga
     3421 gggccaagta gatcggtcgg gctagaatca gcccacccag caagatcgcc gtgtagacat
     3481 ggatgtgcac gtcgctatag gcgccatacc aggtgcgggg cgaaacaacc agcgccacaa
     3541 gcaaacctgc tatccactcg cagatgagca ggttggcaaa gagatggtct gtccgggcgt
     3601 gtacagcacc caacatctca tggaagagtt tctggctctt gctctcaatc tctttgtcta
     3661 tcacctgttt gatagcctct tcagtcattt tgtaaagctg agctccctga ttgactttcc
     3721 agggggaatt gttttgtttg ctctgtcaga gagtgctgaa gtttcactta actgctctga
     3781 gtcgtgcaag agtggacaac cgaagatagg gcagacgtag gttccttcag cagttgcttt
     3841 cacgcctggc tgaatttcca cacctgtcga tttcttcccg gagtttacag acgagatgat
     3901 ttcgagcatg tctttgccgg cgctatctcc ttggtgtccc cgccccggtg tgattccacc
     3961 gcagaaccgc aagtggccgc ttttgtcata aagcagaact tctcccgatg taacagcttt
     4021 gaagtttctg gtttcctggg cattagtgtc ggtgattatg gcgagaccag gtattgatct
     4081 taagcgatcc agcgcctccg tgttagcgaa ctccgggggc gctcccgcag gaatcgtcaa
     4141 aactgcaatg acctgtgttt ccctcaggcg cggtactagt ctggccagct cagaaatggt
     4201 agctgcactg caggggcact tgggatggag aaagaccagc agaatttgct tgtccagggg
     4261 cttttcaagt t
//
LOCUS       DAOQNZ010000139         4231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9337_length_4231_cov_3.890565, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000139 DAOQNZ010000000
VERSION     DAOQNZ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9337_length_4231_cov_3.890565"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1062
                     /locus_tag="PKW73_03630"
     CDS             139..1062
                     /locus_tag="PKW73_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72401.1"
                     /translation="MKISRFGLVSFLIACSIFPASNVYGKDDLGRRLAEADALSHNGH
                     YIEARQIVQSILRKYPREKEAYIILGNSYLNMDDNGESLGRKGYKQAVESFEKARAID
                     PEYGKTYDNLAECAVMNGDYKEAVVLSTKALTVKQPCYSGYRQRCIAYEALGKYDLAI
                     KDIDAYLKTGTFNRSDQVKYQMLKAGILENAKRFDEALAIYKNCESLQNEDTVKLRMI
                     GCLRKAGRARDAIGVTNAMLKANSEDEFALQLRAKLKVEAGDLKGAIDDYSRAIDSLP
                     TASLLNERAALYDKTGRSDLAKKDRKRASGL"
     gene            complement(1330..1731)
                     /locus_tag="PKW73_03635"
     CDS             complement(1330..1731)
                     /locus_tag="PKW73_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72402.1"
                     /translation="MTIYEMHCFNPNLLWQIPSFLIFVAFLYKLFSSLLTSFHASQSK
                     SLVQTMAQKLQYLEKAEVKLHMIKSLPVEGSAYDLRISEARECLRQANTAMEHKNWSS
                     AYRNLQAMRRILIEYNIGHIPPYEMPKPLPA"
     gene            complement(2166..3500)
                     /locus_tag="PKW73_03640"
     CDS             complement(2166..3500)
                     /locus_tag="PKW73_03640"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72403.1"
                     /translation="MPTDRQKSAEVFDTSMDTPGPESDYAQAYSRARESGGFGDYATA
                     ADEFAMAAAVARDFGEQDERLMDSLSRLAYCHYRLGALQEAEQAYREALSLMEKFHKS
                     KHPDRFASILWALAVLLCDNKRYDESEEYFLRSMAVSESWSGPNDRFVADCLWGLSKC
                     LTGAGKFDQAESAILQAISIYESLSRSENVDEFLCTNYNNLGTLLLQRGKLEGSIKYL
                     KKAIKLRQRMAGQYDPGLISLTSKISLALMQVKDYEAARRYLKQGLKVTAAIYGDESI
                     DASRKMIALANCLTITNNCEQAQKYLQKARTILQNQEEEADPTLLTACLFELANCHQK
                     LNQQAKLKDTLEAISDRYEKNRCLARGSMYADAMIKLAEMHSDQCRYKKARQLLLGAL
                     ETRQSLYGREHKSVAECLLRLGNVESRMSLAAEAEEHIRTAEKMLADLKKRA"
     gene            complement(3579..3833)
                     /locus_tag="PKW73_03645"
     CDS             complement(3579..3833)
                     /locus_tag="PKW73_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72404.1"
                     /translation="MIGIFDLAFFLGFFLLTVGAVYFHWLTAPHGVRRRGSVGANYWG
                     TYAEAQKVKSQGSFKLPKPSNNLSSTTIGKTPPGSGGGRR"
     gene            4004..>4231
                     /gene="rnpB"
                     /locus_tag="PKW73_03650"
     ncRNA           4004..>4231
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKW73_03650"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          976 a          938 c         1207 g         1110 t
ORIGIN      
        1 aaagaggaag agcttttcaa aagagagcgg attctaaagg atgagtgtct ttttccggga
       61 agtgagtgtt tgatctggga aaggtgcgca ccgggctaag taggctatga tggtaaggcc
      121 acctatcagg taagagactt gaaaattagt cgattcggat tggtatcctt tttgatagct
      181 tgctcaattt ttcctgcatc aaatgtatat ggaaaggatg atcttgggcg ccgtctggca
      241 gaggctgatg ctctctcgca caatggacac tacattgaag ctcggcaaat tgtgcagtct
      301 atattgcgca agtatccgcg tgaaaaagaa gcatatatta ttctcggcaa tagctatttg
      361 aacatggatg ataacggtga gtctcttggt cgcaagggct ataagcaggc ggtggagagc
      421 tttgagaagg cgcgcgccat agaccctgag tatggaaaaa catacgataa cctggcagag
      481 tgtgctgtca tgaatggtga ctataaagag gcggtagttc tctctaccaa ggctctaacg
      541 gttaagcagc cctgttactc aggctatcgg cagagatgta ttgcatatga ggctcttggt
      601 aaatacgacc tggctatcaa agatatcgat gcttatctga agacgggaac ttttaaccgc
      661 agtgatcagg tcaaatatca gatgctgaaa gccggcattc tggagaatgc caaaagattc
      721 gatgaggcgc tggcgatata caaaaattgc gagagcttac agaatgagga cacagtcaaa
      781 ttgcggatga ttggttgcct tcgcaaagcc ggacgcgcca gggacgcaat cggcgtaacc
      841 aatgccatgc tgaaagccaa ttccgaggac gaatttgctc tgcagctcag ggctaagttg
      901 aaagtggagg ccggcgatct gaagggcgcc atagatgact attccagagc catagatagc
      961 ttgcctacgg catcactgct gaacgaacga gccgcgctct acgataagac cggcaggtcc
     1021 gatctagcta agaaggatag aaagcgagct tccgggcttt agtattgctt ttcaagcaag
     1081 tcgtgtggag ccgggactgg agagcccgag tcagttgacc agagtagata atagaccggt
     1141 ctgttatttg ccgagaaaaa atggtaagta gtagttgaga gctcctcatg cgagatccat
     1201 tttgcatgag gagtattgaa gtgagaattt tccttctatg gaaaatctgg cccggtttag
     1261 ctatggaaaa ctgctagcgc atgtctcgct tgtgatgggt cggaggttct tgccgtgccg
     1321 ggtgaactgt caggccggta atggctttgg catttcgtag ggtgggatat gcccgatatt
     1381 gtattcaatt agaatccgac gcatggcctg caagtttcgg tatgcgctgc tccagttctt
     1441 gtgttccatt gccgtgttgg cctgtcttaa gcactctctg gcttcgctta tccgtaggtc
     1501 gtaggcgctc ccttccactg gtaggctttt gatcatgtgt aatttgactt ccgccttctc
     1561 caggtactgc agtttttgcg ccatggtttg tacaagcgat tttgattgag aggcatggaa
     1621 actagtcagc agagagctga agagtttgta caggaaggca acgaaaatga ggaaggaggg
     1681 aatttgccag agcaggtttg gattgaaaca gtgcatttcg taaattgtca ttttgttttc
     1741 tctcgaaggg cggaggtcac tcctttcctt aagggacaat ccgctggatt taccggcgct
     1801 ccgcgcctgg ttgtgggatg ttgtcttgct ttgatcgcat cgctgattcc agtaaaaatg
     1861 cagcagcgca aaagggagca aaccatcggg cggcaagata attgctgcca tgggatggtt
     1921 tgtgtttggc ggtttgaatt tctaagtttg gtagtgctgc tttgaagagt gaagatggaa
     1981 tcagtatttc agggttaaca tccggcttct attcaagaca agaataaatt acttcttgta
     2041 ggaattagta atagcgcagc ttgaactttg agcgctgcta aagatgctga ttctatggga
     2101 gttggcggta gctttacccc gacaagattg atagagtctc ttttgatggc gggaaagtgt
     2161 accggtcaag cccgtttctt caaatctgcc agcattttct cggcggtgcg gatgtgttct
     2221 tctgcttcgg ccgcaaggga catgcgcgac tctacattac ccaaacgcaa aaggcattcg
     2281 gccactgatt tgtgctctct gccgtacagg gattgtctcg tttccagcgc ccccagtaga
     2341 agttgtcgag cctttttgta gcgacactgg tcactgtgca tttccgccag tttgatcatg
     2401 gcatcggcat acatcgagcc gcgggccagg cagcgattct tttcgtagcg gtcgcttatg
     2461 gcttccaggg tgtctttcag ttttgcttgt tgattcaact tttgatgaca attggccagt
     2521 tcgaaaaggc aggcagtaag caaggtaggg tccgcttcct cctcctggtt ttgcaaaatg
     2581 gttctggcct tctgcagata tttttgggcc tgttcgcagt tgtttgtgat ggttagacaa
     2641 ttggccagag cgatcatttt gcggctggca tcgatgcttt cgtcaccata gatggccgcc
     2701 gtcaccttca atccttgttt gagatagcgc ctggctgctt cgtagtcttt gacctgcatg
     2761 agggccagag atatcttgct ggtcaggctg atcagccccg ggtcgtactg cccggccatg
     2821 cgttggcgca gttttatggc tttcttgagg tatttgatgg acccttccaa tttgccgcgc
     2881 tgcaagagca gggtgcccag attgttgtaa ttggtgcaaa ggaactcgtc tacattttcc
     2941 gagcgggaga ggctttcgta aatgcttatg gcttgcagga tggcgctttc ggcctggtcg
     3001 aatttgccgg caccggtgag gcacttgctc agaccccaga ggcagtcggc tacgaagcgg
     3061 tcgttcggtc cggaccagct ttcagaaaca gccatggagc gcaggaagta ctcttccgac
     3121 tcgtcgtaac gcttgttgtc gcagagcagg acggccaggg cccagagaat ggaggcaaag
     3181 cggtccggat gcttgctctt atggaacttt tccatgaggg acagggcttc ccggtaagcc
     3241 tgttcagctt cttgcagggc gccgaggcga tagtggcagt aggcaaggcg tgagagactg
     3301 tccatgagcc gttcgtcttg ttcgccaaag tctcgggcca cagcggctgc catggcgaat
     3361 tcgtctgcgg cagtggcgta atcgccaaac ccgccgcttt ctctggctct tgagtaagcc
     3421 tgtgcgtaat cgctctccgg accaggtgtg tccatagagg tgtcaaagac ttccgcactc
     3481 ttctgtctgt ctgtcggcat acaaatatca tgcccgcaag acagagtttt tgccccggcg
     3541 ccggaaagtt ttatgtgttc tttgactact aattcggctt agcgtctgcc gccgccggaa
     3601 cccggaggtg ttttgccgat ggttgtggag gaaagattgt tgctgggttt gggaagcttg
     3661 aagctgccct gtgattttac tttttgagcc tctgcatagg tgccccagta gttggcgccg
     3721 acggagcctc gtcgccttac cccatgaggg gcggtaagcc agtggaagta cacggctccc
     3781 actgtgagga gaaagaagcc cagaaagaaa gctagatcga atataccaat catgttctta
     3841 ctcactcctt aaggatgcac tgagatgcta taaacactcg gtctttgcgc tctgcttggc
     3901 atcgcttagt agtttgtaac acaggattga ccccttggct agtaacagaa tgtggaaaac
     3961 cgtatagaga tgttaagaga tttccaacct gtataattca tttggagcag cgcagactgt
     4021 cgcgtcagca atggcgagga aagtccgggc accacagggc tggttgctgg gtaacgccca
     4081 gtgcgcgtga gcgtgaggat agtgccacag aaatgaagac cgcctcgctt gtcgaggtaa
     4141 gggtgcaacg gtgcggtaag agcgtcacca gcagagtcga gaggctctgg ctaggtaaac
     4201 cccgctgaag gtgcaaggcg aaggtataac t
//
LOCUS       DAOQNZ010000140         4211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9416_length_4211_cov_4.276468, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000140 DAOQNZ010000000
VERSION     DAOQNZ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9416_length_4211_cov_4.276468"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKW73_03655"
     CDS             <1..197
                     /locus_tag="PKW73_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72405.1"
                     /translation="DGLDELGRLQLGGLENRKRALCDVQAALAISPDDDDLKDLQDLY
                     RAVIANLERKKNSASKDFNR"
     gene            273..1790
                     /locus_tag="PKW73_03660"
     CDS             273..1790
                     /locus_tag="PKW73_03660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA72406.1"
                     /translation="MSFFAPTAPAATSKSESKRDLIVLGLLIILGLCLRLYTLDSQGF
                     FIDEIYSVLVARGDADPELMAFDSIRPLYFALLGGWMHLGQSELWLRLPSVVFGVLNI
                     VLTYCLGKVSAGHRVGLVAALLMTLSPLEVHYSQQARMYTMGSFLALASSLLLVLAWQ
                     RQSLLALLSWGLSRVLMIWTLPLTALLLVVDVFLSVLLQRKDKLFAPFALVFVLVSVG
                     ALVFAWKMPLISMFNAYDAWRGTLPVPGLLEMIMVPVNFTSCAGPIQECLGPQEGGPL
                     ALLYSAVLLGLTLLSGFVVKEKRALLFPLAWASFTVLFAWAVSQTGTCFLIVRYLLFA
                     APYAFIAMAAGFDFLCSKSRIAAALLSMLYAYIVPLNLYSLTVHPVHEDWRLATRYIE
                     QNEKAGDTILVWTYHSSYVWNYYYQGLNRERVKDFQVNLPEGGKQLQVGALAADNRLS
                     PIKGRTWIVIRQSIKNWDHDWRIYQSVLAHLKERYHLLKSQSVTKLDVILLEERK"
     gene            complement(1791..>4211)
                     /locus_tag="PKW73_03665"
     CDS             complement(1791..>4211)
                     /locus_tag="PKW73_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72407.1"
                     /translation="TSAVQTMMITKDMVVTLPVGQVTIAEVPTVCASVKTVPPPPKDG
                     VNFEVGPYADAKLWRQLSAIIAAASELDRSGAFASVPINTGRIDQIAQLAVWRVLGLA
                     SGKPEHAVTPETIQADLLKAVADQVKKNPELLKQLGDGYELSPKGELIVTKRQKKALD
                     ERVASIFDAVDLTVKRSTDPGLKSVASLPQDSTWDTYVNVGERSYHKGDYVEAEELLS
                     QAVQEAEVFGEADARLSRSMTSLAKCYLDLSWWEKAEALFNRALKLREKVTGASSPEV
                     AEVSQYLGLLKQHQKLYGAAEEQFKRALDIFEKAAGASSKAVAEVLNNLGKNFYLQDD
                     NTSAEPLFKRALAIMLLDQQQTQDKGFPSLPSSEVAEVETNLADSYCKSGKYEEANAL
                     YKKALAIDSKTLGEEHPYVATILDGLANVSTWLNQKDAAELYKKKAQEIREKTLGKEH
                     EQIATLPLGTEALTRIKLYTAGAKAISSSLESVKAGASTIAGASTDKAKINRPIKDKW
                     ALVIGISEFQDTSINLKYAAKDARDFAQFLVKECKFAPDHVRLLINKNATRENILAEI
                     GDKWLPRVANPDDLVLIFVSSHGSPSKVDLNGVNYLVAHNTDKNSLYATGVPMQDLMR
                     MIKDRVHSDRVVLMMDACHSGAATPTKGLFRISNFSADELAQGSGQLVVCSSEPTQSS
                     WESKRYDNGVFTYHLMNALRQNGGMAKLGDAFKYLQDNVQNEVLRDRGELQTPLLKSK
                     WTGDDLILSAPPLQPRPGLEEDSKIFGLEEKVVPPQAVKSIPVTNKVKAAAPQGKPIK
                     PAQKKGSK"
BASE COUNT          802 a         1070 c         1152 g         1187 t
ORIGIN      
        1 tcgacggtct tgacgagctc ggccgtcttc aactcggcgg cctcgagaat cggaagagag
       61 cactgtgcga tgttcaggct gctcttgcta tttccccgga cgatgatgat ctgaaggatt
      121 tgcaagacct atacagagcc gtaatcgcca acctggagcg gaagaaaaac tccgcgtcaa
      181 aagattttaa tcgctgatct tgcccggtct ttagtgactt gctgctatct tgatttactg
      241 ccacagtcgg ggactgaatc ggggatttcg cagtgtcatt ttttgcaccg actgctcctg
      301 ctgcaacttc taagtctgag agcaagcgag acctcattgt gctcggcttg cttatcatac
      361 tagggctgtg cctgcgtctt tatactcttg atagccaggg ctttttcatc gatgaaattt
      421 atagcgtgtt ggtagccagg ggcgatgccg atccggagct aatggccttt gactcgattc
      481 gaccgcttta ttttgccttg ctcgggggct ggatgcacct ggggcaaagt gagctctggt
      541 tgcgcttgcc ttctgttgta tttggggtct tgaacattgt cctgacctac tgcttaggta
      601 aggtttcggc cggtcatcgg gttggtttag tggctgcttt gcttatgacc ctgtcaccac
      661 tggaggtgca ttacagccag caggcaagga tgtacaccat gggtagcttt ctggctctgg
      721 cctccagtct tttacttgtg ctcgcctggc agagacagtc cctgcttgcc cttttgagct
      781 gggggctatc tagagtgttg atgatctgga ctctgccatt gacggctttg ctcctggtgg
      841 ttgatgtttt cctctctgtt ttattgcaaa gaaaagacaa gctgttcgcc ccctttgctc
      901 tggtatttgt gctcgtttcg gtgggcgccc tggtttttgc ctggaagatg ccgctcattt
      961 ccatgttcaa cgcctatgat gcctggagag gcaccttgcc cgtgccgggg ctgcttgaaa
     1021 tgataatggt gccggtcaat ttcaccagtt gcgccggccc cattcaggaa tgtctgggac
     1081 ctcaggaagg cggacccctg gcgcttctct attctgccgt gctgctaggg ctgaccttac
     1141 tttcaggctt tgttgtgaaa gagaaaagag ccttgctctt tccccttgcc tgggcttcct
     1201 tcacggtgct cttcgcctgg gcggtttcgc agaccggaac ctgcttcctc atcgttcgct
     1261 atcttctctt tgccgctccc tatgctttca ttgccatggc ggccggattc gacttcttgt
     1321 gcagcaaaag ccgaattgct gccgctcttt tgtctatgtt gtatgcctat attgtgccgc
     1381 tcaatctcta cagtctcacc gtgcatcccg tgcacgaaga ctggcgtctg gctactagat
     1441 atattgaaca gaacgagaaa gccggtgata ccattctggt ctggacctat cactcaagct
     1501 atgtctggaa ttattactat cagggcttga accgggagcg ggtgaaagac tttcaggtga
     1561 atcttccgga aggtggtaaa caactgcaag tcggtgccct ggccgcagat aacaggctga
     1621 gccccattaa ggggcgtacc tggattgtaa tcaggcagtc tatcaaaaac tgggaccatg
     1681 attggagaat ttaccagagc gtgctcgctc atctgaagga aagatatcac cttctcaaat
     1741 ctcagtctgt caccaaactt gatgtcatac ttttggaaga gcgcaaataa ttacttgctg
     1801 cccttcttct gtgccggctt aattggtttg ccttgcgggg ctgcggcctt gaccttgttc
     1861 gtgactggaa tactctttac ggcttgtggt ggtaccactt tctcttccag tccgaatatt
     1921 tttgaatctt cttcaagacc gggacgaggt tgaaggggcg gtgccgatag aatcaggtca
     1981 tcgccggtcc atttactttt gagcagcggc gtctgcaatt caccgcggtc tctcagcacc
     2041 tcgttttgca cgttatcttg caggtacttg aaggcgtctc ccagtttcgc catgccgccg
     2101 ttttgcctga gggcattcat gaggtgatat gtgaagacac cgttgtcgta acgcttcgat
     2161 tcccaggaac tctgggtcgg ttcgctggag caaactacaa gttgacctga gccctgggcc
     2221 agttcgtcgg cgctgaaatt ggagatgcgg aagagacctt tcgtgggagt ggccgcgccg
     2281 ctatggcagg catccatcat caagactact cggtcggaat ggaccctgtc tttgatcatg
     2341 cgcatcagat cttgcatggg cacaccggta gcatagaggc tgtttttgtc tgtgttgtgg
     2401 gcaaccaagt aattgacgcc gttgagatcg actttagaag gcgagccgtg gctactcacg
     2461 aagattaaga cgagatcgtc gggattggct acccggggca accacttgtc tcctatctct
     2521 gccagaatgt tttccctggt ggcattctta ttgatcagga ggcgcacatg gtcgggagcg
     2581 aatttgcatt ccttgaccag gaactgagcg aaatctctag catctttggc ggcatatttg
     2641 aggttgatgc tggtatcttg aaactcgctt attcctatga ccagggccca tttgtctttg
     2701 atggggcggt tgattttggc tttgtcggtg ctggcgccgg caatggtgga cgcaccggcc
     2761 ttcacactct caaggctgga tgaaatggct ttggcaccgg ccgtgtagag tttgatgcga
     2821 gtgagagcct cggtgccgag aggcagcgtg gcaatttgct cgtgctcttt gcccagggtt
     2881 ttttctctaa tttcttgagc tttcttcttg tatagctcgg cggcgtcctt ctggttcaac
     2941 caggtggaaa cattggccag accgtccagg attgtggcca cataggggtg ctcttcgccg
     3001 agggtcttag agtcgatggc cagcgctttc ttgtagaggg cattggcttc ttcgtacttg
     3061 cctgatttgc agtaagagtc tgctagattg gtctctactt ccgctacttc cgaagagggt
     3121 aggctgggaa agcctttgtc ctgagtctgt tgttgatcaa gtagcatgat ggccagggcc
     3181 cttttgaaga gaggctcggc gcttgtgttg tcatcctgta gatagaagtt ttttcccagg
     3241 ttgttcagta cttcagccac ggctttgctg gaagcgccgg ctgccttctc aaatatgtcc
     3301 agggcccgtt tgaattgttc ctccgctgcc ccatacagtt tctggtgttg tttgagcagt
     3361 ccaagatatt ggcttacctc agcaacttcc ggtgaggaag cgccggtaac tttctctcgc
     3421 agttttaggg cccgattgaa taaggcttcc gctttttccc accaggacag gtccaggtag
     3481 cacttagcga ggctggtcat actgcgcgaa agacgggcat cagcttctcc aaagacctcc
     3541 gcttcttgca ccgcctgcga tagcagttct tccgcttcca catagtcgcc cttgtgatag
     3601 gaacgctcgc ccacattgac ataggtgtcc caggtggagt cctgcggcag gctggccaca
     3661 cttttcagac cgggatcagt actgcgtttg actgttaaat cgacagcgtc gaagatagaa
     3721 gcaactcgct catccagggc tttcttctgg cgctttgtga ctatcagttc gcccttgggg
     3781 ctgagttcat agccatctcc aagttgcttc aaaagctccg ggtttttctt cacctggtcg
     3841 gctacggctt tcagcaaatc cgcctggatg gtctcggggg tgacggcatg ttccggtttg
     3901 ccggaggcca gacccagtac tcgccagacg gctaactgag caatctggtc tatgcgtccc
     3961 gtgtttatcg gcacggaagc gaaggcgccg gaacgatcca actcggaggc agccgcaatg
     4021 attgctgata actgccgcca gagtttggca tcggcatagg gtccgacttc gaaattgaca
     4081 ccgtccttag gcggcggtgg tacagtcttg acggaagcgc acactgtcgg tacttccgca
     4141 atggttacct gccctaccgg tagggtgacc accatgtctt tagtgatcat catggtttgc
     4201 actgcggatg t
//
LOCUS       DAOQNZ010000141         4192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9494_length_4192_cov_3.146966, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000141 DAOQNZ010000000
VERSION     DAOQNZ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9494_length_4192_cov_3.146966"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="PKW73_03670"
     CDS             complement(<1..1236)
                     /locus_tag="PKW73_03670"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA72408.1"
                     /translation="MDQVAPADDLSSFLAILPPNIRQTLGNDTTGLYEVVLDLGRLPE
                     ARYLDRHTVNLSDEPITAQDLEAAIALVGQFSGDNRAGIERTLHRISALRNRSGKIIG
                     LTCRVGRAISGTINVIRDLVESGKSILFLGPPGVGKTTKLREMARVLSDELSKRVIVI
                     DTSNEIAGDGDVPHPAIGRARRMQVARPEEQHATMIEAVENHTPEVIIIDEIGTEEEA
                     QAARTIAERGVMLIGTAHGNALDNLLKNPTLVDLVGGIQTVTLGDDEARRRGTQKTVL
                     ERSNQPTFDICVEILDRQTLAVHRNVGEAVDQLLRGWQIHPEIRKRDESTGDFAVVQK
                     PEPTVTVTMMEGLEPFASDWASPKETHLKVYPYAVSKGLLERVVKTLQLPVEVVKAID
                     EADAILALRSYARAGAKIFS"
     gene            complement(1365..2894)
                     /locus_tag="PKW73_03675"
     CDS             complement(1365..2894)
                     /locus_tag="PKW73_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA72409.1"
                     /translation="MEENSTASDFLSAPRACASAPESSNPAGSAAHDLSLAQAVSEHI
                     KKERASFHTPGHKGRLSELSPSLSLCARGDLTELPGLDDFSNPQGVLRRLEQRAAAIF
                     GSGASLLSLGGASTAIMAAIHSARELGDSIVVPRNAHRSVIEALCFTDLRPIWYEPEF
                     DEDWQVYSKVSPETLESVFDEAQKTSKVALVVVVSPTYAGARSDLEAICKLTRARGLS
                     LLVDEAHGAHLACVHPGRSTTQTSGQAPPVTLSALAAGADLVAHSLHKTLSGLTQTGL
                     LHIGKNSIFAAEQVREQLLRLTSSSPSYLLLASIEAALDLVESARGQHLLLELEELGR
                     KARGAIEQIDCYNIYRQAGTAHDTRIETSASHIFIKPNQGNCTQLQQHLLSQGLYPES
                     LLGNGLLLLLGIGSQTKDLEALITALKTYKPASSQTPRLEPPGQNPALKPSAMVCPLT
                     PFQTSQARKISIARGAALGRTAAEFLAPCPPGIPILIPGQEITTEVLESTNRERFLVV
                     A"
     gene            3590..4189
                     /locus_tag="PKW73_03680"
     CDS             3590..4189
                     /locus_tag="PKW73_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72410.1"
                     /translation="MKPPTFCTLATLCAGACFVASEVLAQQIEELPPRPHYKSICPRP
                     DHFSKEYLEKLQANVQQHWKSQPCTSEIEHLCFYCTVDKFGRIRPASVDDKHPRSIPK
                     ERRQVVADFFSSLPRVDKGTYQPILGSEEVVRTLPDGRKFRQFVFKRGKPIPISESNN
                     ADNELLIQFRKFPDVAMREAWPQELSNVQPIQLPAELMP"
BASE COUNT          898 a         1008 c         1220 g         1066 t
ORIGIN      
        1 ggagaagatt ttggcaccgg cgcgggcata actacgcagt gccagaatgg catcggcttc
       61 atcgatggcc ttcactactt ccacgggcag ttgcagggtc tttaccaccc gctccagaag
      121 ccccttactg acggcatagg gatagacctt gaggtgggtt tctttgggag aggcccagtc
      181 tgaggcgaag ggctccagac cttccatcat agtgacggta acggtcggct ccggcttctg
      241 caccacggcg aagtcgccgg tggactcgtc tctcttgcgg atttccggat gaatctgcca
      301 gcctctcagg agctggtcca cagcttctcc cacgtttcta tgcaccgcca gggtctggcg
      361 atcgaggatc tcgacgcaga tgtcgaaggt cggctggttg gaacgctcca gaaccgtttt
      421 ctgggtgccg cggcgccgtg cttcgtcgtc gcccagggtg actgtttgaa tgcctcctac
      481 caggtctacc agggtggggt tcttcaataa gttgtcgagg gcgttaccgt gagccgtgcc
      541 gataagcatg acgcctcttt cggcaatggt tctggcggcc tgcgcttctt cttcggtgcc
      601 tatctcgtca atgatgatta cttccggcgt atggttttct acagcctcaa tcatcgtggc
      661 atgctgttct tccggacggg ctacctgcat gcgtctggct ctacctatgg ccggatgcgg
      721 tacgtcgccg tcgccggcga tttcattgct ggtgtcgatg acgatgacgc gcttactcag
      781 ctcgtcggag agcactctcg ccatttctct cagcttggtg gttttgccca ctcctggcgg
      841 tccgaggaag agaatggatt tgccggactc gaccaggtcg cggatgacat tgatggtgcc
      901 ggaaatagca cggccgaccc gacaggtaag accgattatt ttgccggaac ggttgcgcaa
      961 agcggaaatt ctgtgcaggg tgcgctcgat accggcacgg ttgtcgcctg agaattgacc
     1021 gaccagggcg atggctgcct ccaggtcttg agcagtgatg ggctcgtcgg agagattgac
     1081 agtgtgcctg tcgagataac gcgcttccgg caggcggccg aggtcgagaa ctacctcgta
     1141 gagaccggta gtgtcattgc cgagagtctg gcgtatattg ggcggtagga ttgccaggaa
     1201 ggaggacaga tcatcggctg gcgcgacttg atccattggt tttctccgta ggggacgagc
     1261 ttctatttct gggcacagtt ttttcacagg ctgctcttct cgatttgtac cggctgagcc
     1321 gttaggttgg gagatagcag tagatgagga ttataaaggt ttcctcaggc tactactaga
     1381 aaccgttcgc gattcgtgct ttccaagacc tccgtggtga tttcttgacc ggggatcaag
     1441 atagggattc cgggcgggca gggtgccaga aattcggcgg cggtgcgacc gagggccgca
     1501 cctctagcga tggatatttt tctggcctgg ctggtttgaa aaggagtgag gggacacacc
     1561 atggcactgg gcttcagtgc agggttttga cctgggggct caagccgggg cgtttggctt
     1621 gaagcaggtt tgtaggtttt gagggcggtg attaaagctt ccaggtcctt agtttgggac
     1681 ccgattccaa gcaagaggag cagtccgttg cccagtaagc tctccgggta gagtccctgg
     1741 ctgagcaaat gttgttgcaa ttgtgtgcag ttaccctgat tcggtttgat gaaaatgtga
     1801 ctggcgctgg tttctattct agtgtcgtgg gcggtgcctg cctgtctgta gatgttgtag
     1861 caatcaattt gttctattgc tcctcgggcc tttcttccca gttcttctaa ttccagtaag
     1921 aggtgctgac cgcgggcgct ttccactaga tcgagagcgg cctctatgga agccagtaac
     1981 aggtagctgg ggctggagga agtcaggcgt agtaattgct ctcttacttg ctctgcggca
     2041 aagatgctgt tcttgcctat gtgcaagaga ccggtttgcg tgaggccgga taaggtttta
     2101 tgcagcgaat gggctactaa atcggctccg gctgccagtg ctgatagtgt cacaggcggt
     2161 gcctgacctg atgtctgggt ggtgcttctg ccggggtgaa cgcatgccag atgggcgccg
     2221 tgtgcctcgt cgaccagcaa actcaagcct cttgccctgg tcagtttgca aatcgcttca
     2281 agatcgctgc gggcgccggc gtaggtgggc gataccacta cgaccagggc tactttgctc
     2341 gttttctgag cctcgtcaaa cacactttcc agcgtttccg gactgacctt ggagtagact
     2401 tgccagtctt catcgaattc aggctcgtac cagattggtc ttaaatcggt aaaacagagg
     2461 gcttcaatga cactgcggtg agcattgcgc ggcactacga tggaatcacc aagttcccgc
     2521 gcactgtgga tggcggccat aatggcggtg gaagcaccgc ccagagaaag caaactggcg
     2581 ccgctgccaa atatagcggc tgctctttgt tccagtcggc gcagcacgcc ttgcggattg
     2641 gagaaatcat ccagccccgg caactcggtc aggtcgcccc tggcgcagag ggaaagtgaa
     2701 ggcgaaagct cggaaagacg gcctttgtgg ccgggtgtat ggaaggaggc gcgttctttc
     2761 tttatatgtt ccgaaaccgc ttgagccagg ctgagatcat gagcggcact gccggctgga
     2821 ttactagact caggtgcgga cgcacacgct cttggggcgg ataagaagtc agaagcagtg
     2881 ctgttttctt ccaattagtt gagcccgaaa cggtcgcagt gttaaattgg acaagggttt
     2941 caagggtcgt aacattatca gtatatcacg caatattaca ggtcaaaaca tgcaagaagc
     3001 ctattgcttg ccggtgttga gcatatactc cgggttgagt gtggctatgg attgaccatg
     3061 gatctggggc agctttgggc gtctcagcca gttccggtag aaattactga gagagacgcg
     3121 cgcgtttttt actcacttgc ggcctgatga ctatagttct tatgtctttt cattccctct
     3181 ttggtgttca cgagaagttc atagaggacg gcgtattctg gaaaaacgcg aggcaaggca
     3241 gaatcgtttt atttagatta gtcggggatt ctaaagttta ctccgctcag cgtggcgcgg
     3301 ggtagcatga tgtggtggcg gtggtggtat tgcaggcagt ggcgctaagg cgctgctgcc
     3361 tgctcgctta gttattgttt tagcatgccg tgcctggagc tgtgtgcatt gagttatcca
     3421 tatccatcga ttttggctgc ttttggtaaa gtttaagcct gctggaaata tagcaccagg
     3481 tttccgcgtg attaattgca acgatcacag ttgctacacc acagcgttga ataaaagcaa
     3541 ggtctgggtg gggaatataa gcatggtgac caagaacgcg aattgacaaa tgaaaccacc
     3601 tacattttgt acactggcta ccttatgcgc tggagcttgt tttgttgctt ctgaagtttt
     3661 agctcagcag atcgaagaat tgcctcctag acctcattac aagagtattt gccccagacc
     3721 agaccatttt tcaaaagaat atctagaaaa actccaagct aacgtccaac agcactggaa
     3781 atcccagccg tgcacctctg aaattgagca cctctgcttc tattgcactg ttgataaatt
     3841 tggcaggata aggcctgcca gtgttgacga taagcatccc agaagtattc cgaaagaaag
     3901 aaggcaggtt gtggctgact tctttagttc tttgccgcgc gtagataaag gaacgtatca
     3961 acctatactt ggatcagagg aagttgtaag aaccttgccg gacggaagaa aattccgaca
     4021 gtttgttttc aaaagaggca agcctattcc tatctcagaa tcaaacaacg ccgataatga
     4081 gctactcatt caatttagaa agtttcctga tgtggccatg agagaggcat ggccgcagga
     4141 actgtccaat gtccagccaa ttcaactacc ggcggagtta atgccataga at
//
LOCUS       DAOQNZ010000142         4176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9550_length_4176_cov_3.621694, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000142 DAOQNZ010000000
VERSION     DAOQNZ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9550_length_4176_cov_3.621694"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3945
                     /locus_tag="PKW73_03685"
     CDS             <1..3945
                     /locus_tag="PKW73_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNA72411.1"
                     /translation="LSHHLKSAKIVIVRALGDSGSIAGLSEILAAGEKTGAALLMLSG
                     TGEPNADLACLSTVSSEILEATRHYFACGGVANLKECLTFLADSLLFTSFGYEAPRTL
                     PDHGFYHGSKDCLKLLTDFKTENYRGVVAILFYRAHWLSGNLKFVDAIISALEEHGLC
                     AIGIYTASLRCLNEEGKALALAQLDDYGITIDTIINTTSFSSSEYGETPQSVLQENSA
                     PVLQAICSTMTMEQWKNSSRGLSPLDTAMNVVLPEFDGRIITHPVCFRGQDGESAALY
                     ESPADRVDRLAKLTARIVRLRKLPNQEKKVAFVLTNSSSKASQIGNAVGLDSPASLIV
                     LLKEMQAQGYAFKKVPDDGDKLIHDLIALCSYDEQFLTEEQTRQCLGKVTRHDYLEFF
                     RTLPEALQKSMVEKWGEPPGEAYLDAENNLLVAGFNLGNAVVILQPPRGYGMDPNAIY
                     HKPDLPPTHHYAAVYLWINQYFGADAVVHMGKHGTLEWLPGKGVGLSQNCFPDALFGD
                     TPLIYPFIINDPGEGSQAKRRSHAVIVDHLTPPMTTADNYGVLSQLVQLVDEYYQVEL
                     LDPAKLPLLQQQIWQLMQEASLEHDLAAVIGHELSDHDHDHEHDHDHEHDHDHEHDHD
                     HDHEHDHDPDHDHEHDHDPDHDHDHDHDHEHDHDHDHDHDHDHDHDHDHDHDHDHEHD
                     HDHDHSHAAEALPLNEVGVPESLASMNASRVSHVIQEIDGYLCELGVAEIRGGLHILG
                     APPQEEKLVDMLFALTRLPNLQLPALPQAVAAYLDLDLETFLKKPGQKLAARPAQLER
                     LSRPIFTAGDGLEAIYQLSRELLDDLLKSRFQAGNLQQTIENRLGKGLRESAAESLES
                     VLVYICRTLVPDLAKTSQEIQNILAALDGRFIPPGPSGAPTRGMAHVLPTGRNFYSVD
                     PRSLPSASAWNVGKQLAGQVLEKYLKENKVYPESVSLSAWGTSAMRTSGDDIAQVLYL
                     MGVEPIWQAENRRVIGLKIIPLNELKRPRINVITRISGFFRDAFSHLIYLVDDAVRLV
                     ANLDEPEEMNYVRKHYLHTLKELKEAGVPATQAEERAAYRIFGSKPGSYGAGILGLIQ
                     EKNWQDEHDFANTYLNWGGYAYSRSSAGREARDDFARGLTQVEIAIHNQDNREHDIFD
                     SDDYLQFHGGMIATIYSLTGRKPKQYFGDSSDPGRGRVRDLKEESYRVFRSRVVNPKW
                     LRAMTEHGYKGALELAATVDYLFGFDATAKVMDDWMYEKVATEYVFSEEIRRFIERSN
                     PWALNSISERLLEAAQRGLWEKPKEETLEKLKQAFLDSEARLEGQAKEQAKR"
     gene            3942..>4176
                     /locus_tag="PKW73_03690"
     CDS             3942..>4176
                     /locus_tag="PKW73_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72412.1"
                     /translation="MKLYPLTAIVGQEDLLLALTVCAVNPLVGGVLIRGDKGSAKSTA
                     ARALAQVMPPIERRPGCRFNCRLDKPTEICPMCN"
BASE COUNT         1073 a         1255 c         1064 g          784 t
ORIGIN      
        1 ctgtctcatc atctcaaatc cgcaaaaatc gttattgtgc gtgccctggg agacagcggc
       61 agtatcgccg gtctttcaga aatactggca gccggagaga aaactggtgc ggcactgctc
      121 atgttgagcg gcacaggcga accaaatgcc gatcttgcct gcctttccac cgtaagtagt
      181 gaaatcctgg aagccaccag gcactacttt gcctgcggcg gcgtcgccaa tttgaaagaa
      241 tgcctgacct tcctggctga ttctctcttg ttcaccagct tcggctacga agcgcccagg
      301 acgctgccgg accacggctt ctaccacggc agcaaagatt gcctgaagct tctcactgat
      361 ttcaaaacgg aaaattaccg gggcgtcgtc gccattttgt tctaccgagc ccactggctg
      421 agcggcaatc tcaaatttgt agacgccatc atttcggcgc tggaagaaca tgggctatgc
      481 gccataggca tctacactgc ttcattgcgt tgcttgaatg aagaaggcaa agccctggcc
      541 ctcgcccagc ttgatgatta cggtatcact atcgatacca taatcaatac cacatccttc
      601 tcctcttccg aatacgggga aacgccgcag tccgtactac aggaaaactc cgccccagta
      661 ctgcaggcaa tttgcagtac tatgaccatg gaacaatgga aaaactccag ccgtggactg
      721 agccccctcg atacggccat gaatgtagtc ctgccagaat tcgacggccg catcattact
      781 catcccgtct gcttccgcgg tcaggatgga gaaagcgcgg cgctctacga aagtcctgca
      841 gatagagtgg accgcctggc caaactgacg gcccgcatcg tgagactaag gaagctcccc
      901 aaccaggaga aaaaagtcgc cttcgtcctc accaactcca gctccaaagc ctcccaaata
      961 ggcaatgccg tggggctgga ctcacccgca tcactgatcg tgctgctgaa ggaaatgcaa
     1021 gcacagggct atgcctttaa gaaagtcccc gatgacggcg acaaactcat tcacgactta
     1081 atcgccctgt gctcttatga cgaacaattt ctcacggaag aacagaccag gcagtgcctg
     1141 ggtaaagtga ccaggcacga ctacctggaa tttttccgga cgctgccgga agcccttcag
     1201 aaatccatgg tggagaagtg gggagagccc ccgggagaag cctatctgga tgcagaaaac
     1261 aatctactcg tggcaggttt caatctgggc aatgccgtcg taattctgca accgcctcgt
     1321 ggctacggca tggaccccaa tgctatctat cacaagccgg acttaccgcc cacgcaccac
     1381 tacgctgccg tctacctctg gataaatcaa tacttcggcg ccgatgccgt agttcatatg
     1441 ggcaagcacg gcactctgga gtggctgccc ggcaaaggcg tgggtttatc acaaaattgt
     1501 ttcccagatg ctctcttcgg cgatacccct ctgatttatc ccttcattat caacgaccca
     1561 ggcgaaggct cccaggcaaa acgtagaagc catgccgtca tcgttgatca cttgacgccg
     1621 cccatgacca ccgccgacaa ctacggcgtg ctctcgcaac tggtgcaatt ggtggatgaa
     1681 tactaccagg tggaactgct ggacccggcc aaattgccgc tactgcagca acaaatctgg
     1741 cagctcatgc aagaagccag ccttgaacac gacctggctg cggttatcgg ccacgaactg
     1801 tccgatcatg atcacgacca tgagcatgat cacgaccacg agcatgacca cgaccacgag
     1861 catgatcacg accatgacca cgagcacgat catgaccccg atcacgacca cgagcacgat
     1921 catgaccccg atcacgacca tgaccacgac catgatcacg aacatgatca cgaccatgac
     1981 catgatcacg accacgatca tgatcacgac catgaccacg accacgacca cgatcacgaa
     2041 catgatcacg atcatgacca cagtcacgct gctgaagccc tgcccctcaa cgaagtaggt
     2101 gtaccggaaa gcctggcatc catgaatgcc tcccgggtct cccacgttat ccaggaaata
     2161 gacggctatc tctgcgaact gggcgtagcg gaaatacgcg gcggactgca catattggga
     2221 gcgcccccac aagaagaaaa actcgtggac atgctctttg ccctgacgcg cttacccaac
     2281 ctgcaattac cggctctacc gcaagcggtg gccgcctacc tggacctgga cctggagaca
     2341 tttctgaaaa agcccggtca gaagctggcc gcaagaccgg ctcaattaga gcgactcagc
     2401 cgccccatct tcacggccgg agatggactc gaggccatct accagctcag ccgagaactc
     2461 ctggacgatc tgctcaaaag cagatttcaa gctgggaatc tgcaacagac tatagaaaac
     2521 cgcctgggca aaggtctgag agaaagcgcc gccgagagcc tggagagcgt actcgtttat
     2581 atctgccgca ccctcgtacc cgacctggct aaaaccagcc aggaaatcca gaacatactg
     2641 gccgccttgg atggccgctt cattccgccg ggtcccagcg gagcccccac cagaggcatg
     2701 gctcacgtgt tacccacggg cagaaacttc tattccgtag atccgcgcag tctgccttcc
     2761 gcctccgcct ggaatgtggg caagcaactg gccgggcagg tgctggaaaa gtacctgaaa
     2821 gaaaacaaag tctatcccga aagtgtcagt ttgagcgcct ggggcaccag cgccatgcgc
     2881 accagcggcg acgacatcgc ccaggtactc tatctgatgg gtgtagaacc catatggcag
     2941 gcggaaaaca ggcgcgttat cggtcttaaa ataatccccc tgaacgaatt gaagcgcccc
     3001 cgcatcaatg tcattacccg catcagcggt ttcttccgcg atgccttcag ccacctcatc
     3061 tacctggtgg acgatgccgt gagactggta gcaaacctgg acgaaccgga agaaatgaac
     3121 tatgtacgca agcactactt gcacacactg aaggaactca aggaggcagg cgtgccggct
     3181 acccaggcgg aagaaagagc agcctatcgc atcttcggct ccaaaccggg cagctacgga
     3241 gcgggcatac tcggtctcat tcaagagaaa aactggcagg acgagcatga ttttgccaat
     3301 acctatctca actggggcgg ctatgcctac agtcgcagca gcgccggacg ggaagcccgg
     3361 gacgacttcg ccagaggtct tacccaggtg gaaatcgcca tccataatca agacaatcga
     3421 gagcacgaca tcttcgacag cgatgactac ctgcaattcc acggcggtat gattgcaacc
     3481 atatacagcc tcacaggcag aaagccgaaa caatacttcg gagacagcag cgatccggga
     3541 cgcggtcggg tgcgcgatct aaaggaagaa tcctaccggg tcttccgctc ccgcgtcgtc
     3601 aatcccaaat ggctcagggc catgaccgag catggctaca aaggcgcgct ggaactggct
     3661 gccaccgtgg actatctttt cggcttcgat gccactgcaa aagtcatgga cgactggatg
     3721 tacgagaaag tagcaacaga gtatgtattc agtgaagaaa ttcgccgctt catcgaaaga
     3781 agcaatccct gggcgctcaa ttcaatctca gaaaggctcc tggaagcagc tcagcgagga
     3841 ctatgggaga agccaaagga agagacttta gaaaaactca aacaggcttt cctcgactcg
     3901 gaagcccggc tggaaggaca ggcaaaggag caggcgaaaa gatgaaactc tatccgctca
     3961 cagctatcgt cggtcaggaa gatttgctgc tggccctcac cgtctgcgcc gtcaaccccc
     4021 tggtgggcgg cgtcctgatt cgcggcgaca agggtagtgc caaaagcact gccgcccggg
     4081 ccctggccca ggtgatgccg cctatcgagc gccgtcccgg ctgtcgcttc aattgccggc
     4141 tggataaacc gaccgaaatc tgccccatgt gcaacg
//
LOCUS       DAOQNZ010000143         4175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9552_length_4175_cov_3.331068, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000143 DAOQNZ010000000
VERSION     DAOQNZ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9552_length_4175_cov_3.331068"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PKW73_03695"
     CDS             complement(<1..1100)
                     /locus_tag="PKW73_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72413.1"
                     /translation="MFDENSKLIDILTHKDNSSLQEKTKNAGMVEDRLKALDKDTKYL
                     ADESAAPIYADILGRALQAKALDDSDFCRLYKKVRGPGARFDKAKSSDAANIAFAAVD
                     NKADIVCKEKKPSPCPKGLRTISDCVKEGVTIDLIGDGSTTTHCLLELTNPATGATTS
                     AQIHTMCISRHTQHPPQPRAEAGTSERYKLGGPAPVELVNIVKAAHKLAEEGRFASVP
                     LPQERKIGTIMQLGIWRHLGGSKGKDKDAVTNQSVTKDLLARAHITEKQLSSDEKKAL
                     TSRVDQILEAVDLTEKTATCLEEGKKPTNTEEPETPPTQDHGTCPGNSQKQASGPNDR
                     QISNPANGPGTPAKPGSLPQQTQPLPPAYNGKV"
     gene            complement(1687..>4175)
                     /locus_tag="PKW73_03700"
     CDS             complement(1687..>4175)
                     /locus_tag="PKW73_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HNA72414.1"
                     /translation="QVILAEKPSVARDIAAVVGAKTKRDGYFEGNGYAVTYAFGHLVT
                     LAEPEDMNPAWGKPWRLAQLPMVPTDWKYRVVEKSASQFNVIKKLFLDKDTASIICAT
                     DAGREGEHIFRLIYRLSGTNKPVERLWISSLTADAIKDGIKKLKPAKEFDNLASAAAA
                     RAHADWVVGLNFTRAYTTINNQLCTIGRVQTPTLALIVERQKTIDNFQSTPFYEILAT
                     FEPGFVARYITPEAEPQTKLKDKAVAQAIVKDITPVKAGVVKSVVTSEKKAKAPALYD
                     LLTLQKEANKRFGYTAQETLNLAQSLYEEHKLISYPRTESRHLSNDMVEELPRIVSSL
                     LKAPTITKKVKDAFAAEGIVPGSITAKLLSPKLSKAYVDDTKLTDHHAIIPTFNTPGS
                     LPDKQRNIYDLVVARFLSIFLPPEVRDETTIILSLDKHDFRARGVVIKVEGWTVMEPK
                     AKEKEKKKSKDEDEEAQQLPALTVAQNVPKQKAELKERKTNPPKPYDDSSLLTAMKNA
                     GQELDDEDLASYMKQKGLGTPATRAAIIERLLQTGYVERNKKSLVPTIKGKTLIEQVH
                     QDLKDIALTANWEQKLADMQDGKMERESFEKDIADFVCRILPDVTSDTGAMVRAGSGI
                     GLCPLCKKGVVRPTPKGAGCNRWKEGCAFNIWREQRGKSLTDAQIKELVEKRSTKVIK
                     GFKKKDGSGTYDARLVINEEGKVVFSLDSAEGQEEVDFGACPQCKEGKLRMSPKAVGC
                     SRWKEGCNFTIWRTQYGKELSVEIIKELLEKKETGVIEGFQKKAGNGSYSARLVMGED
                     FKVKLDFAAPRAQETQPNMQKESPEQAVTE"
BASE COUNT          784 a         1067 c         1069 g         1255 t
ORIGIN      
        1 accttgccgt tgtaagccgg cggcagaggc tgggtttgtt gcggtaaact gccgggtttt
       61 gccggggtgc ctggaccgtt agcagggttt gaaatttgcc ggtcattggg cccgcttgct
      121 tgtttctgcg aattacccgg acaagttcca tgatcttggg ttggaggcgt ctctggttct
      181 tctgtattcg tcggcttctt cccttcttcg aggcaagttg ccgtcttttc ggtcaagtcc
      241 accgcttcta gaatttgatc tacgcggctg gttaatgcct ttttctcgtc cgaagatagt
      301 tgcttctcag tgatgtgggc tcgcgccagt aaatctttcg tgaccgattg gtttgtcact
      361 gcatccttgt cttttccttt gcttcctccc aggtgacgcc atattccaag ttgcattatg
      421 gtgccgatct ttctttcctg cggcaaaggc acagaagcga acctgccttc ctctgcaagc
      481 ttgtgggctg ctttgacgat attgaccagt tctaccggtg ccggtccacc cagcttatat
      541 ctttcactcg tgcccgcttc agctcgtggt tgtggtggat gctgggtgtg cctggagatg
      601 cacatagtat gaatttgagc ggatgttgtg gcgcccgtag cagggttggt taactcaagt
      661 aggcaatggg ttgtggtact tccatcgccg attagatcta tggtcacgcc ttctttgacg
      721 caatcgctta tggttctcaa accttttgga cagggcgaag gcttcttttc cttgcatact
      781 atgtcggcct tattgtccac ggcagcaaat gcgatattgg cggcgtctga cgacttcgct
      841 ttatcaaatc tggctccggg cccccgtacc tttttgtaaa ggcggcaaaa atcgctatca
      901 tcaagggctt ttgcttggag ggcgcgcccg agaatatcgg cataaattgg ggccgctgat
      961 tcatcggcca gatacttcgt gtctttgtcc agcgctttga gcctgtcttc taccatgccg
     1021 gcatttttgg ttttttcctg caggctgctg ttgtctttat gggttagtat gtcgatgagc
     1081 ttgctgtttt catcgaacat tttagtgtcc tgcgcaatta ccggcagtgg cacgagtaat
     1141 tgtttcaaac aagtgatcgt gagcaacaag gtgataatta gaaaggagtg tcgattcttc
     1201 tgcattgatt ctttccgcca gcattgactg taaagccgca gggctgctaa gtcttgctca
     1261 gccccctgta tggacacccg cgcctgtgcg cgtgacgtaa gtcttacggc tcatagtttt
     1321 gtagccgtca gaattttcag aaaattgatt ccaagaagag ttcaatgatg ttccctgccg
     1381 gatgtcgccg gcacaccgct tggctaaccg tcagcaaggc atgggaactc gtcgaacgac
     1441 ttcagatggt gcttatgata cagcatcttt cctggtcggt tcggtcacgg ttttagaggc
     1501 tcatccctat ccctgtccac cgcgctcggg tgccgctaga atgtcgtttt cgttaattaa
     1561 attctatcgg tctatcccga tagctttgta tagcttgata gtcagctgta ccggtagccg
     1621 gctttgaaat gaagcacagg tgaacggcgg tagtcaggag ttaggtggta tttttgccgg
     1681 accggactat tctgtaacgg cctgttccgg agattccttt tgcatattgg gctgcgtctc
     1741 ctgggcgcgg ggagcggcaa aatccagttt taccttgaag tcttcaccca tgaccagtct
     1801 ggcgctatag ctgccgtttc cagctttctt ttgaaagcct tctattacgc cggtctcttt
     1861 cttttccaag agttccttga taatctccac gctcagttct ttaccgtact gagtacgcca
     1921 gatggtgaag ttgcagccct ctttccagcg actgcagccc actgctttgg gcgacattct
     1981 cagcttgcct tctttgcact gtggacaagc accaaaatct acttcttcct gcccttctgc
     2041 ggagtccagg ctgaagacaa cttttccttc ttcatttatc accaggcgtg catcataagt
     2101 accggagccg tctttctttt tgaagccttt aattaccttg gtgctgcgct tttctaccag
     2161 ctctttgatt tgagcatcgg tcagcgattt gcctcgctgc tctctccaga tattgaaagc
     2221 gcaaccttcc ttccagcggt tgcagccggc tcctttaggg gtggggcgca ccactccttt
     2281 cttgcacaga gggcaaagcc ctatgccgga accggctctt accatagctc cggtgtcgct
     2341 ggtcacatca ggcaaaattc tgcagacaaa gtcggcaatg tctttttcaa aactttctct
     2401 ttccatcttg ccgtcttgca tatcggccag tttctgctcc cagttagcgg tgagcgcaat
     2461 atcttttaga tcctggtgca cctgctcgat taaggtctta cctttaatgg tgggcaccag
     2521 gctcttcttg ttcctctcta catagccggt ctgtaaaagc cgctcgataa tggcggcacg
     2581 agtagccgga gtgccaagcc ctttctgctt catatagctg gccagatctt cgtcgtccaa
     2641 ctcttgcccg gcatttttca tggctgtgag caaactggag tcatcgtatg gcttgggcgg
     2701 attggttttt ctttctttca attccgcttt ttgcttgggc acgttttgag ccactgttag
     2761 agcgggaagc tgctgcgctt cctcgtcttc gtccttgctt ttcttcttct ctttttcctt
     2821 ggctttcggc tccatgaccg tccagccttc cacctttata actacgcccc gggcgcggaa
     2881 gtcgtgtttg tcgaggctca gaataattgt ggtctcatcg cgcacttccg ggggcaggaa
     2941 gatactcaag aatcttgcca ccacaagatc gtatatattg cgctgtttat caggcaggga
     3001 acccggcgta ttgaaggtag gaatgatggc gtggtgatca gtgagcttag tatcatccac
     3061 ataggctttg ctcagcttag gactcaatag tttggcggta attgagccgg gaacgatacc
     3121 ttcggcggca aaagcgtctt ttaccttctt ggtaatggtt ggtgctttca gcaaggaaga
     3181 cacgatgcgc ggcagctctt ccaccatatc gttggaaaga tgtcttgatt cggttctcgg
     3241 ataagagatt agtttgtgct cttcatagag gctctgagcc aggttcaggg tctcctgggc
     3301 ggtatagccg aagcgcttat tggcttcttt ttgcaaggtc aaaaggtcgt agagggccgg
     3361 tgccttggcc ttcttttcac tggtgaccac cgactttacc acccctgctt tcacaggcgt
     3421 gatgtctttg acaatagcct gggcaacggc tttgtctttc agtttggtct gaggttcggc
     3481 ttccggtgtg atatacctgg ccacaaagcc cggctcaaag gtcgctagaa tttcgtaaaa
     3541 aggcgtagac tgaaaattgt ctatggtctt ttgtctttca acaataagag ccagtgtcgg
     3601 tgtctgcaca cggcctatgg tgcagagctg attattgatg gttgtatagg ctctggtgaa
     3661 gttcagaccc acaacccagt cggcatgggc cctggctgca gcagcactgg ccaggttatc
     3721 gaactctttg gcgggcttca atttcttaat gccgtccttg atggcatctg ccgtcaggga
     3781 actaatccac agcctttcca ccggtttgtt tgtgccggaa agcctgtaga taagtctgaa
     3841 tatgtgttcc ccttcccttc cggcgtctgt ggcgcagatt atggaggcgg tgtctttgtc
     3901 gaggaagagc tttttaatta cattgaactg gctggccgat ttttccacca cccggtactt
     3961 ccagtccgtg ggtaccatgg gcagttgagc cagtcgccag ggtttgcccc aggctggatt
     4021 catgtcctcg ggttcagcca gggtgaccag gtggccgaag gcatatgtaa cggcatagcc
     4081 attgccctca aagtagccat ctcttttcgt ttttgcccct accacagccg cgatatctcg
     4141 ggctacggag ggcttctccg ccagaattac ctgca
//
LOCUS       DAOQNZ010000144         4172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9559_length_4172_cov_4.875880, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000144 DAOQNZ010000000
VERSION     DAOQNZ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9559_length_4172_cov_4.875880"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PKW73_03705"
     CDS             complement(<1..671)
                     /locus_tag="PKW73_03705"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HNA72415.1"
                     /translation="MTFDLCQWLRQQCASEFSSVPVQGISLENLTAADDREWLVTNGL
                     GSYAGAAISGANTRRYHGLFVGSLNPPVQRLVFFSRLDERVCTSGQNGVLELGCNIWR
                     SGAVAPQGHKHLLAFAAAPVPTFCYQLPEGKLIKQVLMKDGEQHVYIGYSYVSDLAES
                     EISLDLSVLLNYRDFHSQTRGSDDWHFSQEKQTPGGVKVTAYNGAEPFFLCSNSGEYQ
                     VDSSW"
     gene            complement(1026..1613)
                     /locus_tag="PKW73_03710"
     CDS             complement(1026..1613)
                     /locus_tag="PKW73_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72416.1"
                     /translation="MICPGNSALAQGFPGKGEPGAWSDALPHYNLGNRYLEHERLDEA
                     IEQYQVAISLYQYDPDFYINLGYALRKIKDYAGAEKAFRKALSLNDKDWLSWSNLANS
                     LLKQDRLPETIAAFEKCLKCAPPAKEKEAILKDIADIKKVLSFRNSPPPASKPAAVIR
                     KTVKAPPGKGSSGSGSSASKSGSGAVDKGGWDYVN"
     gene            complement(1860..2726)
                     /locus_tag="PKW73_03715"
     CDS             complement(1860..2726)
                     /locus_tag="PKW73_03715"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNA72417.1"
                     /translation="MEGWKRESRRRAGPSIETLCRVLEVSCSGYFKWKKRKVTPRQTK
                     RALLLKRILEIFEESRETYGCPRMYEKLRAEGFTCSYRTVEKLMSEHEVRPTRKRKYK
                     ATTDSKHKLPVAPNVLDRQFTVSEPDEVWVSDITYVDTAQGWLYLCVFIDLYSRSVVG
                     WSMATHMETSLVSDAFLMGVGRRGRAPIVEHSDRGSQYASLDFRKLLDQHGTIQSMSR
                     KGNCWDNAVAESFFGALKSELIYRNRFETRIQATMAIFDYIEIFYNKRRLHSTLGYLT
                     PWEKEQKVRKAA"
     gene            complement(2738..3016)
                     /locus_tag="PKW73_03720"
     CDS             complement(2738..3016)
                     /locus_tag="PKW73_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNA72418.1"
                     /translation="MTGKKKPSEYSREFQENAIRLTNMPGRTVASVAVELKIPAWKLR
                     TWIKNSRDNKERSEDLNELNRLTNEVKSLKEEIEILKKAAAYFAKNLA"
     gene            complement(3124..3813)
                     /locus_tag="PKW73_03725"
     CDS             complement(3124..3813)
                     /locus_tag="PKW73_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72419.1"
                     /translation="MLSRANLLKIPIPSWILGWVFAAEPTDRLIARSERNALFKIPYT
                     RKVLHLVSRQTPMSYLRVFFLVMLLYMSLSSAFAEGSKTTAEEIQKLRSHCILPDSIL
                     AKPPQDISDCGQREYILHNDIWVRRPESRCCVALIRISENGKAVLNPDGFLYGPIPNI
                     AIITRQQADALWQSEPVPTTGPRVSYKLSSFNDNPEDVYIDVVFENNKVDKYRLRAKR
                     ITIQEWQSVTR"
     gene            complement(3911..>4172)
                     /locus_tag="PKW73_03730"
     CDS             complement(3911..>4172)
                     /locus_tag="PKW73_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72420.1"
                     /translation="FADRLEYEPVFPSPGQILTEIDMSFFWFLLYCKFPLLMILGLTG
                     AFGTCAKEVSEYWCTCIGIGITFLAACFAIYSIYMWLAGLFT"
BASE COUNT         1000 a         1060 c         1014 g         1098 t
ORIGIN      
        1 taccaggacg agtcgacctg atactcacca ctgttgctgc aaagaaagaa cggctcggca
       61 ccattgtaag cagtaacttt gacaccgccc ggcgtttgct tctcctggct gaagtgccag
      121 tcatcgctac cacgagtctg gctgtgaaag tcacgatagt tgagcaagac ggacaggtcg
      181 agcgagatct cactctcagc cagatccgaa acatagctgt acccaatgta gacgtgctgt
      241 tcgccgtctt tcatgagcac ctgcttgatc aacttgccct ccggcagttg atagcagaag
      301 gtaggcaccg gggcggcggc gaaagccagc aaatgcttat gcccctgcgg agccacagcc
      361 cccgacctcc agatgttgca acccagctcc aggacgccgt tctgcccgct ggtgcaaacc
      421 ctctcatcga ggcgtgaaaa gaaaaccagc ctctgcaccg gcgggttgag agaacccacg
      481 aagaggccgt gatagcgcct ggtattggca cctgaaattg ctgcgccggc ataggaaccg
      541 aggccgtttg tcaccagcca ttccctatcg tcggcagccg ttagattttc caaactgatg
      601 ccctgaacgg gcactgatga gaattcggat gcgcactgtt ggcgcagcca ctggcataaa
      661 tcaaaagtca tgatagtcac cgtttgagac gttgtcaggc caagtcagcc tgttgttaag
      721 cttgaggccc tactgaaccg gttatgtggt tatagagggt ggaaaaggaa agttattggt
      781 cgatacaacc tggacaaagg aaataagtta aagagttaga tacttgtcca gcttagctat
      841 atcaagcact tattttttag ggaaaactag ttagaaacct ttcaaattca cggctccgct
      901 tcgcgcagct aggcccgtct tatcgatagc aaggtcctct tgtatacatc agggtttcca
      961 agagaggtgg tgcgaggaaa acggaagcag ttctcacaaa ctgctctgcc ggtcaacaaa
     1021 gaacatcagt tgacgtagtc ccaaccgcct ttatcgacag ctccggaacc cgacttagat
     1081 gcagacgagc cggaaccact gcttcctttg cccggcggcg ctttgacagt tttgcgaata
     1141 accgcagccg gtttacttgc cggcggcggc gaatttctaa agctgagcac cttcttgata
     1201 tctgcgatat ccttcaggat ggcttctttc tccttcgcag gaggagcgca cttcaaacat
     1261 ttttcgaaag cggcaatggt ctccggcaag cgatcttgct tgagcaggga atttgccaga
     1321 ttgctccagg aaagccagtc tttatcgttg aggctgagag ccttgcgaaa ggctttctcg
     1381 gcaccggcat aatctttgat tttgcgcagg gcataaccca agttgatgta gaaatcaggg
     1441 tcgtattgat aaagcgaaat agcgacctgg tactgctcaa tggcctcatc aaggcgctca
     1501 tgctcaagat agcgattgcc cagattatag tgaggcaggg cgtcgctcca tgctccgggc
     1561 tcgcccttgc ctggaaaacc ttgagccaga gccgaatttc cgggacaaat caaactgaac
     1621 gctactaccg caaaaaaaac taggccaatt aacttgcttg cctgccgtgc ctgtccttct
     1681 gttgccaatc ctgcttgcat gctgttcaac cgcacggctc ccgcacctcc gtcacaagtg
     1741 tagatcttac cgcctcaagg ctcaggagcg ataaatctaa accaagacta caccgatgtg
     1801 aataaagcgt ggtggtgggg ttcccccctt ttagtagagt gtaggccggc tcgatcgcct
     1861 caggcggctt tccgtacttt ttgttctttt tcccagggtg tgagataacc caacgtcgaa
     1921 tgaagcctcc ttttgttgta gaagatctcg atgtagtcga agatagccat ggttgcctga
     1981 attcgggtct caaagcggtt acggtaaatt agctcggatt tgagagctcc gaagaagctc
     2041 tcggcgacgg cgttgtccca gcagttgcct ttgcggctca tgctttgaat cgtgccatgt
     2101 tggtctagca gctttctgaa gtccaggctg gcgtattggc tgccacggtc tgagtgctca
     2161 acgattgggg ctcgtcctcg tcttccaact cccatcagga aggcgtcaga gactagactt
     2221 gtctccatgt gagtagccat ggaccaccca accacagacc tactgtacag gtcgatgaag
     2281 acacacaggt atagccaacc ctgagccgtg tcaacgtaag tgatatcact cacccagact
     2341 tcatcaggct cagagactgt aaactgtctg tcgagtacat taggcgccac tggtagcttg
     2401 tgcttggagt ccgttgtagc cttgtatttg cgctttctag ttggacgaac ttcgtgttcg
     2461 ctcatgagtt tttcgaccgt cctgtagctg caagtaaagc cctctgcacg gagcttttcg
     2521 tacatcctcg gacatccgta ggtttctctc gactcttcaa atatctccag aatgcgcttc
     2581 aggagcaagg cacgcttcgt ctgcctgggt gtcactttgc gtttcttcca cttgaaatag
     2641 ccggaacagc taacttccag gacccggcac agggtctcaa tgctaggtcc agctcttctc
     2701 ctggattcgc gcttccagcc ttcgatgaaa gcgtacttta cgccaggttc ttcgcaaagt
     2761 acgctgctgc cttttttagg atctcgattt cttctttgag gctcttaacc tcgttggtga
     2821 gccgattcaa ctcgttcaag tcttcacttc tttctttgtt gtccctggag ttcttgatcc
     2881 aggttctgag tttccatgcc ggtatcttaa gttctacggc cacggatgca actgtccgac
     2941 caggcatgtt tgtaagcctg atcgcgttct cctggaactc tcttgaatat tctgaaggtt
     3001 tcttcttgcc agtcattttg cgctcctttg gtatttttcc acatggagcg atctctgcct
     3061 agaactaaac tctacttttt ggggggaccc tcaactaacc aaagctaagt aaactactaa
     3121 gctttatctt gtgacggatt gccattcttg aatggtaatc cgttttgctc tcagtctgta
     3181 tttatctacc ttgttgtttt caaatacaac gtcaatgtac acgtcttctg ggttgtcgtt
     3241 aaaacttgaa agtttgtagc ttactcgcgg tccagtcgtt gggaccggct ctgactgcca
     3301 tagcgcatca gcttgctgtc ttgttatgat cgcgatattg ggaataggcc cataaagaaa
     3361 tccatctgga ttcaaaactg ctttcccatt ttctgaaata cgaattaatg ccacacagca
     3421 acggctttcc ggccgcctca cccaaatgtc gttgtggagt atatattccc gctgaccaca
     3481 atcagaaatg tcttgaggtg gctttgccaa aatactgtct ggaaggatgc aatgggaacg
     3541 aagtttttga atttcttccg ctgtggtctt gcttccttct gcaaaggcac tcgaaaggct
     3601 catgtatagc agcatcacaa gaaaaaagac tcttaaatag gacataggtg tttgccggct
     3661 gaccaagtgg agaacttttc tagtataggg tatcttgaag agagcgtttc tctcactcct
     3721 tgcaattaat ctatcagtag gctcagctgc aaatacccaa cccaatatcc aagaaggaat
     3781 cggtattttg agcaggttgg cgcgagaaag catctactat atgcaggcgt aaaaatttaa
     3841 tttttgagaa agtgagatac aggtgggtag cttcttgcaa atgagctacc cgcctgattt
     3901 gtccgcatct ctaagtgaag agtccggcca gccacatgta gattgaataa atggcaaagc
     3961 aggctgccaa aaaggtaatg cctattccta tacaggtgca ccagtattct gatacttcct
     4021 ttgcgcaggt tccgaaggct ccggtcagtc cgaggatcat gagcagagga aacttgcagt
     4081 aaagtagaaa ccaaaagaaa ctcatatcta tctccgtgag aatttgccca gggcttggga
     4141 agaccggctc gtattcaagc cggtctgcga ag
//
LOCUS       DAOQNZ010000145         4160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9606_length_4160_cov_3.410719, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000145 DAOQNZ010000000
VERSION     DAOQNZ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9606_length_4160_cov_3.410719"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKW73_03735"
     CDS             <1..651
                     /locus_tag="PKW73_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72421.1"
                     /translation="QSHCYWGGSETIVSMDGMRLENKGRMHFIVVASRPTWDVTIYRD
                     DDKVYIKESLKEFESTGLVSEFLVTKQARKFDGVGEKFTAKIGDLEVSEVRGPKEQFA
                     YLPLGKLAAPEVERILYTVFKQSTDGGIPIKYARTQKGVDWLTGLKRSGQLRNVLYTS
                     GLQKVKVNKEIFEAPVGYKRAASVQEVLMSKVSRDASGDMDEIFEIGGKNRSAKPR"
     gene            814..1875
                     /locus_tag="PKW73_03740"
     CDS             814..1875
                     /locus_tag="PKW73_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72422.1"
                     /translation="MTSAIDKPGIKYKFAWMFGRNNDLLFYFAPILLALGLYAAVQNN
                     IVTAGLLFLIVANGLGLNQFHLGPSWYFYFDKKNLEHWRSDKGRAMLFFGGPIIILTV
                     CTLLGAVQPGILFLLTTLWGIQHFIQQNFGILILYHNPNSGEAIASRTLQSRSLWSAS
                     VFFSIFYFEKLVFKGGGIAIFAAVAAVLALVAIFYCALYLKDLMGQVKQGASLNVPAL
                     LFWFMGVVYFAPFALADYNETTAFIIPGVMHWSQYMFLNYMLVKYKYQGEESKQLPMA
                     PMLLFFGLAVLVFVLSFSLQNVRLSGTFVNPIVGFLLGLSNVHYFQDAFLWRFREQFQ
                     RQSILPYLKQARMIESGTQ"
     gene            1862..3685
                     /locus_tag="PKW73_03745"
     CDS             1862..3685
                     /locus_tag="PKW73_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72423.1"
                     /translation="MEPSSALAGASKEHLRPSPFKNIFESRRSFVLFLQILLFPLFFV
                     LGLLVPGLRSYVGTYVPVVSMADTVSQAVFCYWVCLSVVCLALVVRNLRTYYDGESLS
                     VRVAVFEAGYHFLMLYIVATRTLQLQPLGDDAFIDYRYATHWINGMVDFNSTERVMGF
                     TAHLHIFLLSVLAFISRLTGVSLEQLSIIANCFLQCLSLSYLLRFLRLIVRNHVPVIL
                     AGAVFALSPFEMFASAIGKEQPLGVFLMVVALLAFTRKQAGIFAWSSTALALVRPEGI
                     FFLALAVLFSVKQMGRKWLRPWLLPVSLFALYHLALFCYFGSPFPHAAIVKSQIYTKR
                     LFPYAAFIELVRHIGTNSIGCVPYKETYAIGKGAIYLVPGGLLILLIAFGLKGSRVFR
                     LYVLVVIAVLLFYSIPNSWIFNWYLLWFALLPVLLPVFIFQALSTRGKAALVLSYAIF
                     AGTILAQISMYPRIKVVGLDLPLPLFLYQEEWGRFAVYREIGLLIKEMAAPGDLVAVT
                     EPGLVGYYYGGPILDLGGLVSHCVVSCYSKLPRAETPDLLYAPPPEAISKFKPRFICF
                     QDGLVTREFLESPYFKENYVLIKFWPLPIYQKTGLFLYQAK"
     gene            complement(3721..>4160)
                     /locus_tag="PKW73_03750"
     CDS             complement(3721..>4160)
                     /locus_tag="PKW73_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNA72424.1"
                     /translation="MTCSMKNLFGTVPGRKYGWPKNMLHIKGIPHSIIDLQHLVKPKL
                     AVVDAIVAMEGDGPINGTAKPFGYLVVGEDLAAVDATCARMIKLEPNDLPYIKMAGKV
                     VGNIESKDIDLLGPAIADIAQVFALPITMRDKSLLKQAAEQGS"
BASE COUNT          817 a         1033 c         1083 g         1227 t
ORIGIN      
        1 cagtctcatt gttactgggg aggctcggag acaattgttt ccatggacgg catgcgtctg
       61 gagaacaagg ggcgaatgca cttcattgtt gtggcttctc gtccgacctg ggacgtgaca
      121 atttaccgcg atgacgacaa ggtttacatc aaagagtcgc tgaaagagtt tgagtccacc
      181 gggctggttt cggagtttct tgtcaccaag caggcgcgca agttcgatgg tgtaggcgag
      241 aaatttacag ctaagatcgg ggacctggaa gtatccgaag tgcgcggccc taaagagcag
      301 tttgcttatt tgcccctcgg taaacttgct gcaccggagg tagagagaat tctctacacc
      361 gtcttcaagc agtccaccga tggtggcatt cctatcaaat acgcaagaac tcaaaaagga
      421 gtggactggc tgaccggtct caagcgcagt ggtcaattgc gaaatgtgct ttacaccagt
      481 ggtcttcaaa aagtgaaggt gaataaagag atttttgagg ccccggtggg ttataagcga
      541 gcggcatcgg tgcaggaagt gctgatgagc aaggtcagtc gcgatgcctc aggcgatatg
      601 gacgagatct tcgagatcgg tggtaagaat cgcagtgcca agccgcgctg aaatcgagtc
      661 agatctggtc tgtgcacttg ttaccttttt gtgaccggtg gcggcccgta ttagcaaaag
      721 attgcaacaa ttgcccttca ggcttaaatg ttggtacaaa ctccagggtg acgtggaata
      781 ctaagactgt tcggatcttt aggaataaga cctatgacca gcgccataga caagcccggc
      841 attaagtata aatttgcctg gatgtttggg cgtaacaacg acctgctttt ttatttcgct
      901 cctattcttc tggcactggg cttgtatgcg gcagtgcaga ataatatcgt tacggccggg
      961 ctgctctttc tgattgtggc caatgggctg ggtctcaacc aatttcatct gggaccgtcc
     1021 tggtatttct atttcgacaa aaagaacctg gagcactggc gcagcgacaa gggtcgcgcc
     1081 atgctttttt ttggcggtcc gataataatc cttaccgttt gcactttgct gggagcggtg
     1141 caaccgggca ttttgttcct tctgactaca ctctggggca ttcagcattt tattcaacaa
     1201 aatttcggca ttctcattct ttaccacaac cccaatagcg gcgaagccat agcctcccgt
     1261 actttgcagt cgcgcagcct ctggtcggct tcggttttct tttcgatttt ctatttcgaa
     1321 aaactggttt tcaaaggtgg aggtattgct atatttgccg cagtcgccgc cgttttggct
     1381 ctggtggcaa ttttttattg tgccctctac ctgaaagacc ttatggggca ggttaagcag
     1441 ggcgccagtt tgaatgtgcc ggctctgctt ttctggttca tgggtgttgt ctatttcgcg
     1501 ccctttgccc tggctgatta caacgagacc acggccttca tcattcctgg tgtcatgcac
     1561 tggtcgcagt atatgttcct gaattacatg ctggtcaagt acaagtatca gggtgaagag
     1621 tctaagcaac tgccgatggc accaatgcta ctcttcttcg ggctggctgt gctggttttt
     1681 gtactttcct tttccctgca aaacgtgagg ctttccggta cttttgtgaa tccgattgtc
     1741 ggctttctcc tcggtttgtc caacgtacat tacttccagg atgccttctt gtggcgcttt
     1801 agagagcagt ttcagcgcca gtccattttg ccttacctca agcaagcacg gatgattgag
     1861 agtggaaccc agtagcgccc tcgccggcgc gagcaaagaa catttgcgac cgtcgccatt
     1921 taagaacatc tttgagagcc gccgcagctt tgtgctcttt ttgcagatct tgcttttccc
     1981 gctctttttc gtcttgggtt tgctggtgcc cggcttgcgt agttatgtgg gaacttatgt
     2041 gccggtggta tccatggccg atacggtttc tcaggcggtc ttctgctact gggtttgtct
     2101 ttccgttgtt tgcctggcgc tggtggtgcg caacttgcgc acttattatg acggagagag
     2161 tctcagtgtg agagtggccg tctttgaagc aggctatcac tttttgatgc tgtatatagt
     2221 ggcaacgcgc accttgcaac tgcaacctct cggtgatgat gcctttatcg attatcgcta
     2281 tgccactcac tggataaacg gtatggtgga cttcaacagc acggagcggg tgatgggctt
     2341 cacggctcat ttgcatattt tcctgctaag cgtgcttgcc tttataagtc gccttaccgg
     2401 tgtcagtctt gagcaacttt ccattatcgc caattgtttt ctacagtgcc tgtcgctctc
     2461 ttatctgttg cgcttcctgc gcttgatcgt gcgcaatcat gtgccggtga tactggccgg
     2521 ggcggtcttt gccctcagcc cctttgagat gtttgcttcg gccatcggta aagaacagcc
     2581 tctgggcgta tttctcatgg tggtggcctt acttgctttt acgcgcaagc aggccggaat
     2641 atttgcctgg tcatccacgg ccctggcttt agtgagaccg gagggcattt tctttctggc
     2701 actggccgtg ctcttctctg tcaagcaaat ggggagaaaa tggctccggc cctggttact
     2761 tcccgtatct ttgttcgcgc tctatcatct ggcattgttc tgctatttcg gttcaccatt
     2821 tccccatgcg gctatcgtca aatcacagat ttatacgaag agactgttcc cctatgccgc
     2881 tttcattgaa ttggtgcgtc atatcggcac caactccatt ggctgtgtgc cgtataagga
     2941 aacctatgcc attggaaaag gggcaattta tctggttccc ggcgggctcc ttattctttt
     3001 gattgccttt ggcttaaaag gcagtcgcgt ctttcgcctg tatgtgctgg tggtcattgc
     3061 tgttttgctc ttttacagca ttcccaacag ctggattttc aactggtatc tcctatggtt
     3121 tgcacttctg ccggtgcttc tgccggtctt tatttttcaa gctctttcca ccaggggtaa
     3181 ggcggccctt gtgctcagct atgccatctt tgccggtacc atactggctc agatcagtat
     3241 gtatccccgc atcaaggttg tgggattgga ccttcccctg ccgctcttct tgtatcagga
     3301 agagtgggga cgttttgccg tctatcgaga aattggtctt ttgatcaagg agatggccgc
     3361 acctggcgat cttgtggcgg tgaccgagcc cggcctggtt ggttattact acggcggacc
     3421 gattcttgat ctgggagggc ttgtctctca ctgcgtggtc tcttgctaca gcaaactgcc
     3481 ccgggctgag acccccgacc tgctttatgc gccgccgccg gaggctattt caaaattcaa
     3541 gccgcgcttc atttgttttc aggatggtct ggtgaccagg gaatttttag agagccccta
     3601 tttcaaagag aattatgtgc tgataaagtt ctggcccctg cccatttacc aaaagaccgg
     3661 gctctttctt tatcaggcga agtaattggc tgttttgccg gccgcaccgc tttttcctcg
     3721 ctagcttccc tgttcggccg cctgtttgag caggctcttg tctctcatgg tgatgggcag
     3781 ggcaaagact tgcgcaatat cggcaatggc cggaccgagc aggtcaatat ccttgctctc
     3841 gatattaccg acaacttttc cggccatctt tatataagga agatcgttcg gttcaagctt
     3901 gatcatgcgg gcacaagtgg cgtccacggc agctagatct tctcccacaa ccagataacc
     3961 gaagggcttg gcggtgccgt ttatgggccc gtctccttcc atggccacta tggcatcgac
     4021 cacggccaac tttggtttga ctagatgctg caagtcgatt attgagtggg gaattccttt
     4081 tatgtgcagc atatttttgg gccagccgta tttgcgaccc ggtaccgtcc cgaagagatt
     4141 cttcatgctg caggtcatgc
//
LOCUS       DAOQNZ010000146         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9642_length_4149_cov_3.824621, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000146 DAOQNZ010000000
VERSION     DAOQNZ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9642_length_4149_cov_3.824621"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PKW73_03755"
     CDS             complement(<1..856)
                     /locus_tag="PKW73_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72425.1"
                     /translation="MCGIVGYLGPSQAAPVLLNELKCLEYRGYDSAGVAVIEEGRLCV
                     LKAAGKLSNLEALLDNKRPKARVGIGHTRWATHGIPNDQNAHPHTDATGTLAVVHNGI
                     IENHEELREELKGLGYEFASETDTEVIVHLVSHEYAKDQDLLQSILRAVQRLAGIFAL
                     GIVCQKQPDRIYAVNHHYSLSVGLGENENFLASDSMAVRQYTNRVIKLDQSEIAEITA
                     SGVRLFTFDGKEVKRSPIALDASPYVIDKCGYKHFLLKEIHEQPLVLRQTLSKYLQHP
                     NHPIDLNID"
     gene            complement(898..1455)
                     /gene="frr"
                     /locus_tag="PKW73_03760"
     CDS             complement(898..1455)
                     /gene="frr"
                     /locus_tag="PKW73_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNA72426.1"
                     /translation="MTTTTEVMQNADEHMKKALARLHKELQTVRTGRANPQMLDRIEA
                     DYYGTMTPLKGLANISTPDGRSLVIQPYDKGSLKAIEQAIHKSDLGVNPNSDGSVIRI
                     TFPQLTEERRKEMIKMVNKIGEEFRVAVRNARRDADQDLKKMKGEISEDEIKRQMEAL
                     QKLTDKHIKEIDKTLQDKEAELMEV"
     gene            complement(1458..2213)
                     /gene="pyrH"
                     /locus_tag="PKW73_03765"
     CDS             complement(1458..2213)
                     /gene="pyrH"
                     /locus_tag="PKW73_03765"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNA72427.1"
                     /translation="MSEVRFKRILLKLSGEALTGAQGFGIEPDIIAGIADEISSAYSL
                     GVQIAIVVGGGNIFRGVQGSSWGMDKAAADYVGMLATIMNCLILQEVLTRKGIPTRVQ
                     TAIAMPAVAEPFIRLRAIRHLEKGRVVIFGGGTGNPHVTTDTAAALRAAEMKADAILM
                     AKNHVDGVYEEDPKKNPNAKRIDFISFDDMLARQLKVMDPSAVSLCQQNSIPIIVFDF
                     ARQGSIERIVLGDKIGTLIGSECGTEKATGART"
     gene            complement(2318..3220)
                     /gene="tsf"
                     /locus_tag="PKW73_03770"
     CDS             complement(2318..3220)
                     /gene="tsf"
                     /locus_tag="PKW73_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNA72428.1"
                     /translation="MVEVSASMVKELREKSGAAMMDCKKALVEAGGDFEKAFELLRQK
                     GAAAAGKKASRITSEGLVVGQVSECGKVAALVEVNCETDFVARNDEFQALGKELAAVA
                     LKEKPTTVEELLAKQTSKGNTVKELVTEAVAKTGENINVKRLQVFESTDGITGLYIHA
                     LGGKMGAIVELSADKCVKGCTDMQGLAREIAMHVVSAKPSYLDREAVPADMIENERRI
                     EAGKADLADKKPEMREKIVAGRVDKILAERCLTEQPFVKEQSTSVGKYLAAKGKELGV
                     ATKPVRFALFILGEGEGEAQGEEA"
     gene            complement(3477..4136)
                     /gene="rpsB"
                     /locus_tag="PKW73_03775"
                     /pseudo
     CDS             complement(3477..4136)
                     /gene="rpsB"
                     /locus_tag="PKW73_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
BASE COUNT          768 a         1090 c         1139 g         1152 t
ORIGIN      
        1 ggtcgatgtt caaatcgatg ggatggttgg gatgttgcaa atatttggag agggtctggc
       61 gcagtaccag cggctgttca tgaatttcct tgagaaggaa atgcttgtag ccgcatttgt
      121 cgatgacata agggctggca tccagagcga tggggctgcg ttttacttct ttgccgtcga
      181 aggtaaagag tctgacgccg gaagccgtga tttcagctat ctcgctttga tccagcttga
      241 tgacccggtt ggtgtactgg cgcacggcca tggagtcgga agcgaggaag ttttcgttct
      301 cgcccagacc gactgagagc gaataatggt gattgacggc gtatatgcga tcgggctgtt
      361 tctggcaaac gatgccgagg gcgaaaatac cggccagtct ctgcaccgcc cgcagtatcg
      421 actgcaagag gtcttgatct ttggcgtatt cgtgggagac caggtgcacg attacttccg
      481 tatcggtctc ggaggcgaat tcatagccga gccccttcaa ctcttctctc agttcttcgt
      541 ggttttcgat aatgccgttg tgcaccaccg ccagcgtgcc tgtggcatcg gtgtgggggt
      601 gggcgttctg gtcattggga atgccgtggg tagcccatct ggtgtgaccg atacccacgc
      661 gagccttggg tcttttgttg tcgagcagcg cttccagatt ggaaagcttg ccggccgctt
      721 tcaagacgca caatctgcct tcttcaataa cagccacacc ggctgaatca taaccgcggt
      781 actccaggca tttgagttcg ttcaatagaa ccggagcggc ctgggaagga ccaaggtaac
      841 caactattcc gcacattttt atgggcctcg tacaagtaat tccgaagtgg ggaagttcta
      901 gacttccatc agttctgctt ctttgtcctg taatgtctta tcgatttctt tgatatgttt
      961 gtctgtaagc ttttgcagcg cttccatttg acgtttgatc tcgtcttcgg aaatttcgcc
     1021 tttcattttc ttcagatctt gatcggcatc cctgcgggca ttgcgcaccg ccacacggaa
     1081 ttcttcgcct attttgttga ccattttgat catttctttg cgtctttctt ccgtcaactg
     1141 gggaaaggta atgcgtatga cggagccgtc ggagttggga ttgacgccca gatcagattt
     1201 gtggatggcc tgttctatgg ctttcaggga gcctttgtca tagggttgga tgaccaggct
     1261 tctgccgtcg ggggtggaga tgttggccag tcctttgaga ggcgtcatgg tgccgtagta
     1321 gtcggcctcg atgcgatcca gcatttgcgg gttggctctg cctgtgcgca ctgtctgtaa
     1381 ctctttgtgc aagcgggcga gagctttttt catgtgctcg tcggcgttct gcattacttc
     1441 agtggtggtg gtcattctta ggtcctcgct cctgttgctt tttccgtgcc gcattcagat
     1501 ccaattaaag ttccgatttt gtctcccaga acgattcgct cgatagaacc ctggcgggca
     1561 aaatcgaaga cgatgatggg aatcgagttt tgctggcaga gggagactgc cgatgggtcc
     1621 atgactttca attgtctggc cagcatatcg tcgaagctta tgaagtcgat gcgtttggca
     1681 ttgggatttt tcttgggatc ttcttcgtag actccgtcca catggttctt agccatgagg
     1741 atggcatccg ctttcatttc cgccgccctc agggctgcgg ctgtgtcggt ggtgacgtgg
     1801 gggttaccgg ttccaccgcc gaaaataacg actcgcccct tctcaaggtg gcgaattgct
     1861 cgcagtcgaa tgaacggttc agccaccgcc ggcatggcga tggctgtttg tactctggtg
     1921 ggaatgcctt ttctggtcag tacttcctgc agaatcaagc aattcataat agtggccagc
     1981 ataccgacat agtcggcggc cgccttgtcc atgccccagg atgagccctg gacgccgcgg
     2041 aagatattac cgccgccaac aacgatggct atttgtacgc ccaggctgta tgcgctcgat
     2101 atctcatcgg cgatgccggc gattatgtct ggctcgatac caaaaccttg cgctccggta
     2161 agcgcttctc cacttaattt gaggaggatt ctcttaaatc ggacttccga catattccac
     2221 atgcttcctt ttgaaattgg cacccaaagc gagagccggg tcagatcata actctttccc
     2281 ggctcctact tctatagctt tacgctcatc aagttggtta agcttcttcg ccctgggctt
     2341 cgccttcgcc ttctcccaaa ataaatagcg cgaagcggac cggcttggtg gccacgccca
     2401 gttctttgcc tttggcggcg aggtattttc ctacggaagt ggactgttct ttcacgaagg
     2461 gctgctcggt caagcaacgc tcggcaagga ttttatccac gcgaccggca acgatttttt
     2521 cacgcatttc cggtttctta tcggcgagat cggctttgcc ggcttcgatg cggcgctcgt
     2581 tttcgatcat gtcggccggg acggcttctc tgtcgaggta gctgggctta gccgagacaa
     2641 cgtgcatggc gatttctctg gccagacctt gcatgtcggt gcagcctttt acgcacttgt
     2701 cggcggaaag ctcgacgata gcgcccattt tgccgcccag agcatgaatg tacagaccgg
     2761 tgatgccgtc tgtgctctcg aacacttgca ggcgcttgac gttgatgttt tcgccggttt
     2821 tcgctacggc ttcggtgacc aactccttaa cggtgttgcc tttgctggtc tgcttggcaa
     2881 gcagttcttc caccgtggtc ggcttttctt tcaaagccac ggcggccagc tctttaccga
     2941 gggcctggaa ttcatcgttt ctggccacga aatcggtttc gcaattgact tccaccaggg
     3001 ctgccacttt gccgcactcg gatacctggc ccactacaag accttcggat gtgatgcgcg
     3061 aggctttctt gccggccgca gcagcaccct tctgtctcaa gagttcaaaa gctttttcga
     3121 agtcgccgcc ggcttccacc agggctttct tgcagtccat catggctgcg ccggacttct
     3181 ctctcagttc cttcaccatg gaagcggaaa cttcaaccac tgtgctcatc tccttcctaa
     3241 gacctcgtat ttgttattta tgtgtgcaat aaaatctaaa gagcccggct ggcgcctcgt
     3301 ttaagaagcc acagccggga ctcagttgcc cttgactagg cttcggcttc gtcgtcaccg
     3361 aaagcatcgg gagcatcggc gtactcgggg gcagcggcgc ccacggaggc catttgcggt
     3421 tccggttctt tccaggcggc tttctcttca gccggtccgg cgtaggcctg accttgtttg
     3481 ccttccagaa tggagtcggc aatcttgctg gttataagct tgatggaacg aatggagtcg
     3541 tcgttgcccg ggatgacgta atcgatgccg tcgggatcgc agttggtatc gatgatgccc
     3601 actgtggaga tgccgatttt cttggcttcc gaaacagcga taagctcgcg cttctggtcg
     3661 atgatgaaga gcatgtccgg cttgccgcgc atggttttga tgccgcccag cgacttctcg
     3721 agtttgagca gttcgcggtt gagcatagac tgttctttct tgccgcggcg atagaagtcg
     3781 ccggtgtctc tcatctcttc cagctctttc aggcggttga tacgcagtct gatggtttcg
     3841 aagttagtga gcatgccgcc cagccagcgg cgattgacat agtgggcgcc gcagcgtttg
     3901 gcttcttctt ctacaatttc agccgcctgc tttttggtgc cgacgaaaat gatgttcttc
     3961 ttctcggagg ctgcctttct gacaaactca caagcccgat cgagagcgcg agtggtctgc
     4021 tcgaggtcga tgatgtaaat accgttacgt tccccataga tgtatggtct catcttggga
     4081 ttccagcggc gcgtttggtg accaaagtgg acgcccgctt ccaaaagttg tctcatcgat
     4141 gctactggc
//
LOCUS       DAOQNZ010000147         4139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9679_length_4139_cov_4.340597, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000147 DAOQNZ010000000
VERSION     DAOQNZ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9679_length_4139_cov_4.340597"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKW73_03780"
     CDS             complement(<1..186)
                     /locus_tag="PKW73_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72429.1"
                     /translation="MNDLESQFNHTALHFILVTLSYIGFGTLLYTIWPPTPTFAVFVA
                     TAVCTGLGIISISLIFYR"
     gene            complement(304..933)
                     /locus_tag="PKW73_03785"
     CDS             complement(304..933)
                     /locus_tag="PKW73_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72430.1"
                     /translation="MKHQRASRGGAIPLLIILAIGWSGYAIWLFSIWPPHPGPTVIAG
                     AIVMVCIFVFSLSVILHSSFRHQPPPFGLESLPDSLIPKKPVRLENWSLHYHKRKVDQ
                     EADGYVLQGFVYGDPNYADGSEITTGRVDDVIGKVVRIHEYWVILGKPSEAYREHYPD
                     YIPEMPLWGFRDKPIKHHFDFDELERSGRKLPPRELVKAIEAECLRFNN"
     gene            1821..2312
                     /locus_tag="PKW73_03790"
     CDS             1821..2312
                     /locus_tag="PKW73_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72431.1"
                     /translation="MTIKHTSIKSRGFWLVLSAGVLFTLWYGWTLAVSVHLTAADLIW
                     PGLALDLHPLVSPVAFLIAGYLFCEILATEQFRLLPLQALRATACRSLFKMLVHLACS
                     IVAFSAVFLAVLLTSALERQAVVAIVGLSFWTLAVIYFFAFGGPTIKQLGERARRLTL
                     KFQ"
     gene            2323..2580
                     /locus_tag="PKW73_03795"
     CDS             2323..2580
                     /locus_tag="PKW73_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72432.1"
                     /translation="MTNNPNGSHSCTPRKTQVSAAQLRARCVQYLLNMNDAQFSSWIM
                     TEGALFCLEVLPPTMYALTPRSDGSFELAFHETAGLDDKSD"
     gene            2763..4004
                     /locus_tag="PKW73_03800"
     CDS             2763..4004
                     /locus_tag="PKW73_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72433.1"
                     /translation="MTETTDKTLTPSFVAPTTNAAWDAFIAPFAAAIGKDAATVGEAL
                     KPLVGELGAEAVDLLNSEEYTPFEDLRKAFEGVPLARLKKAVADHLRKAKVVPETVVV
                     QAGRAAGYSALPPVPDNGAWLTALKSGGELKVDQTSIYAAIRAGIASRVGFFDLPSML
                     AERMERYAEELEEPASAEYYKLRKLLTRRSYADVLTALDIEGSFVTQSRKNQLLAKLD
                     EQMWPALFSFQNQLKGWVETWQQGSNNPAMFMGMIAMASGGGQLALPPGMMEPPATDG
                     LHDAAESVIQKINRIFAGTGIPVARALAYDANQIMEVLENSSLPAQIGAANREQMLKM
                     LNVAVTNDYPRMERNLIGYVLAIMEFDKVTGGNDEIAYLTDLVRLGMQIPWDKLQAPA
                     RKAGVRSRIEREESDEWSGHR"
BASE COUNT          908 a         1042 c         1118 g         1071 t
ORIGIN      
        1 tcggtaaaaa atgagcgaga ttgagatgat gcccagacca gtgcaaaccg ccgtagccac
       61 aaagacggca aaagttggcg tcggcggcca gattgtgtac aggagtgtac cgaagccgat
      121 ataacttagt gttactagta tgaaatgtaa ggcagtgtgg ttgaattgcg attccaggtc
      181 attcatacac gttttctcct tccccttatc tagggcttgg ggagctaatg ctcggtttca
      241 attgtgaaag gctcggtgct agctgcccaa cgccttcagg caagcgtttg gatgattcag
      301 ctgttaattg ttaaagcgca ggcattctgc ttctatagct tttacaagct ctctgggagg
      361 aagcttcctg ccgctccgtt caagctcatc aaagtcgaag tgatgtttga tgggtttatc
      421 gcggaagccc cagagcggca tttccgggat gtagtctgga tagtgctcac ggtaggcctc
      481 agaaggcttc ccgagtatca cccagtattc gtgaatgcgc acgactttgc cgattacgtc
      541 gtctactctt ccggttgtta tctcagagcc gtcggcatag ttcggatcac catagacaaa
      601 gccctgcagg acatagccgt ctgcttcctg atccactttg cgtttgtggt aatggaggct
      661 ccagttttcg aggcgaaccg gtttttttgg tatgaggctg tctggcagac tttcgagccc
      721 gaaaggagga ggctgatgcc gaaagctgga atgcaggatt accgatagcg agaaaacgaa
      781 gatgcagacc attacaatgg cgccggcaat gacggtaggg ccggggtgcg ggggccagat
      841 gctgaacagc cagatggcat agccactcca tccgatcgcg agaattatta gcagcggaat
      901 agctccaccg cgacttgctc tttggtgctt catagaaata ttctccaccc cctgcttgag
      961 aggttagtag ttgacggtct ttcaattaaa gccgtggatc cggcatcgaa ccgaaagctc
     1021 gagaggtact tcgttgaccc tgtttctgtt ttcctgaaag actcttttga tagaccggaa
     1081 gaaaggttgt ccagagcgag cagcgagctg cttgaggaat ggtgctttca ttggttgctg
     1141 tttgcgcttt aaagctatct agagagagtt ccagttaaat gtcggagtta gggtaattaa
     1201 acctaagaaa tctaagagct catgtcgatg ctctaattca tgatctaagc tggctcgaag
     1261 cagctggagt ttctactgga gaaccattca acgtgggggt tttcggggtg gaatggagga
     1321 gataatgtgc taatcaattc aggctgctgt ttcgtataag ccgcagggca aggttcgcta
     1381 aggagattac acggtgctca ccgtctcatt gagtccaccg gaagcggccg aaccgtgctc
     1441 acggtttcga cacagatgaa acacggatgt gccacagaaa gcaggccgct ttcggctgtc
     1501 cggtgcaggc cggctagtag ctctactagg atcggcctta caagaattgt taagggatga
     1561 gctggagcag gtctttctga agcattttta cacccttggg ttggttgtag ccagagaaat
     1621 taagctctca cttcggtcag actgggtctg ttccattttt ctttccttca ccttagagat
     1681 tttcacccac cttcccgctc ctgcgccaag aaacccgtta cgaagcagcc accgctctgt
     1741 tgaagagttt gtcggggtct gcctggttca tggtgctgaa gaggagggct aggtgaatta
     1801 tcgacgcgga gcaacacttt atgactatta agcacacgtc catcaaatcc agaggttttt
     1861 ggctggtatt gtctgcagga gttttgttca cgctctggta tggctggact cttgccgttt
     1921 ctgtgcatct gactgcagcc gacctgatct ggccgggcct tgccctggac cttcacccat
     1981 tagtttctcc ggtggcgttt ttgattgccg gctatctgtt ttgcgagatt ctcgctacag
     2041 agcaattcag actgctgccg ctgcaagcct tgagagcaac cgcctgccgg tcgcttttca
     2101 aaatgctggt gcaccttgcc tgcagcatag ttgcattcag cgccgttttt cttgccgtac
     2161 tgctcacctc cgccctggag cgccaggctg ttgtagctat cgtcggactg tccttctgga
     2221 ctctggcggt gatttacttc tttgccttcg gtggtcccac gatcaaacag ctaggtgagc
     2281 gggcaagacg ccttaccctc aaatttcaat gaagatacgt caatgacgaa taatcccaat
     2341 ggttcccaca gttgcactcc acggaaaacc caagtcagcg ccgctcagtt gcgcgcaagg
     2401 tgcgttcagt atttgctcaa catgaatgat gcccagttct cgtcctggat tatgaccgag
     2461 ggggcgcttt tctgtctgga ggtcttaccc ccgacaatgt atgccctcac acctcgctcg
     2521 gacggtagtt tcgaactcgc tttccatgag actgccggtt tggacgacaa gtccgattga
     2581 ggtttctatc tcacagagcc ggttttatcc cggctcgttg tttttgtgaa agacggcgat
     2641 ttgtcgctta gaaccacaaa agctttttct agtcgactgt tttcagtcga catagcggtc
     2701 cgcttccgcc ctggaagcgg accaatagtc caattcttaa ctcttaacac gaggaagcca
     2761 aaatgaccga aaccacagac aaaaccctta cccccagctt cgttgctcct acgaccaatg
     2821 ccgcctggga tgcgtttatt gctccctttg ccgctgccat cggtaaggat gcagccaccg
     2881 ttggtgaggc tcttaagcct ctcgtcggtg agcttggtgc ggaagctgtc gaccttctta
     2941 acagtgagga atacacgcct ttcgaagatt tgcgtaaagc ctttgaaggc gtaccgctgg
     3001 ctcgtctcaa gaaggcggta gccgatcatc tgcgcaaagc caaagtcgtg ccggaaacag
     3061 tcgttgtgca agccggtcgt gccgcaggct acagcgctct gccgccggtg cctgataacg
     3121 gtgcctggct gaccgcactt aagtctggcg gcgaattgaa agtggatcag acctctatct
     3181 atgctgccat tcgtgccggt attgccagcc gcgtcggttt cttcgacttg cccagtatgc
     3241 ttgccgagcg catggagcgc tatgccgagg aactggaaga accggccagt gccgagtact
     3301 acaagctgcg caagcttttg acgcgccgtt cctacgccga tgttttgacg gcgctggata
     3361 tcgaaggctc tttcgtcact cagagtcgca aaaatcagtt gctggctaag cttgatgagc
     3421 agatgtggcc tgccttgttc agcttccaga atcaactgaa aggttgggtt gaaacctggc
     3481 agcaaggctc taacaatcct gccatgttca tgggcatgat tgccatggct tccggcggcg
     3541 gacagttggc tctgcctccg ggtatgatgg agccgccggc caccgacggt ctgcacgatg
     3601 cagctgagag cgtcattcag aaaatcaaca ggatctttgc cggtaccggt attccagttg
     3661 ctcgtgcact ggcttacgat gccaatcaga tcatggaagt tttggagaat tcttccttgc
     3721 cggctcaaat tggtgccgcc aatcgcgagc agatgctcaa aatgttgaac gttgccgtga
     3781 ctaacgatta ccctcgcatg gagcgtaatc tgattggtta cgtgctggcc attatggagt
     3841 ttgacaaagt caccggcggc aatgacgaaa ttgcatattt gaccgacctt gtgcgcctgg
     3901 gcatgcagat tccctgggac aagttgcagg cgccggctcg caaagccggt gttcgcagcc
     3961 gtattgaaag agaagaaagc gatgagtgga gcgggcaccg ttaaggctgc tccgtagact
     4021 cctgccggcg gaagataggc tgaatctgct tctcttccgc cctttctgta aactgcaatt
     4081 tctttgggag ttcatctatg gatgccgatt taaattcggt ttacttgcag gtcatgcaa
//
LOCUS       DAOQNZ010000148         4132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9717_length_4132_cov_4.071131, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000148 DAOQNZ010000000
VERSION     DAOQNZ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9717_length_4132_cov_4.071131"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKW73_03805"
     CDS             <1..219
                     /locus_tag="PKW73_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72434.1"
                     /translation="SSKRNVYIGPKGEEFLISPWFQWQGVIFGARHRDLHSYVYSLLD
                     EKDQRENSVFCMRQYHARFDWRINDIHA"
     gene            203..2179
                     /locus_tag="PKW73_03810"
     CDS             203..2179
                     /locus_tag="PKW73_03810"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNA72435.1"
                     /translation="MTSTPEKALERLVPSSLRLLLLFLCICQLNACKAQESPESPQVT
                     PPLKKPTPIKRVKVELTDSNPIYVSYPQGGTTAITASSSFIIGSVLPGGSLSINGKPV
                     SLNKDGFFAQTVPLTRGVNRFQLLYTRDGAQTPYSAFVQLVREKLREPLSPSSLVISG
                     EGVEPGDDRALTAGDIIEFSCHATPGAAVYVELAGKKIEMASLANLRARAKERTGPGI
                     NRGLEAAYGQIFQRFPQHSPDLYLGLYRIENGDFFTNCHPRFVLTKGGKNTSLTLGTN
                     ISVVKQPLMAHTIHDDTIVRVAPELARLTPLPAGVRLITDGYQKDNIRCLYKAGKHVW
                     IKREDLQFEPPGAPAPRAVCRSVQVEKDDWGEIVSIPMSQRLPFLIEQAVSAGSNKLQ
                     VKIYGAQSDTDWVYQSPADGEARLIEDVAWKQPDDGVYEIDIALKPGRQWGYFAEYKE
                     NNLNLHIKYPPRLVSTAEKGRSERPLEGLVICVDPGHGGLESGALGPSGLCEAEVNLA
                     IAKKFKAALEKEGATVFMTREEDIDVSLNDRVAFARDKKVDLLISVHNNALPDGRDPL
                     KEHGTSTYRYHPQSIELARCLKDSMVKELELPDLGARYQNLALCRPTAMQAVLVEVAF
                     MVNPDEYSNLINSQWQNKAARSLVNGLLSYFARK"
     gene            complement(2199..2387)
                     /locus_tag="PKW73_03815"
     CDS             complement(2199..2387)
                     /locus_tag="PKW73_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72436.1"
                     /translation="MQEELIPANLRKRLDDLKDREDSYWHDELYSVEALTNMAYRQEL
                     ERSFKLDPLGPMPPRQQF"
     gene            2738..3049
                     /locus_tag="PKW73_03820"
     CDS             2738..3049
                     /locus_tag="PKW73_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72437.1"
                     /translation="MNLKLRLEKLCDARAWVKLSFADGTALVGRLLRIGQDYVELETF
                     GDPEKRYSSDHASDYSRHLIPLGLIKLLTVESPAFAEYERRRLDYLSRLDSHDAPDLE
                     R"
     gene            complement(3046..>4132)
                     /gene="glmU"
                     /locus_tag="PKW73_03825"
     CDS             complement(3046..>4132)
                     /gene="glmU"
                     /locus_tag="PKW73_03825"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNA72438.1"
                     /translation="IQSATLKHLLLSHEQSGAAVTALTAIVEDPKNYGRIVRDEAGKV
                     RAVVEDKDASPAEKAICEVNTGIYCLSWPAVKDGLQSLTCENKQKEYYLTDLVGWAYK
                     SGAGAAAVALTDWREMAGINSRLELSEAARHLRDITVSRLALESGVTIVDPLSTWICP
                     EVKIGQETTIMPGCILTGDIEIGADCLIGPYTVMKGRVRVGAGASVVNSYVNDSVIGD
                     RARVGPFAHLREGNAVGSASKVGNFVELKKATLGDHTNVGHLSYIGDASLGSNVNIGA
                     GTITANYDHVTKRKDRTIVGDGASTGSNSVLVAPVELCAEAVVGAGTVVTKDVPSGAL
                     AVGRARQEIIEGWAKRRKERMTVAPKA"
BASE COUNT         1003 a         1134 c         1048 g          947 t
ORIGIN      
        1 tccagtaaac gaaatgtcta tatcggtcca aagggagaag agtttctcat atcaccctgg
       61 tttcaatggc agggggtcat cttcggcgca agacaccgcg acttgcacag ttacgtgtac
      121 agcctccttg acgaaaagga tcagcgcgaa aacagcgtat tctgcatgcg ccagtaccat
      181 gctcgcttcg attggagaat aaatgacatc cacgcctgaa aaggctcttg agcgcctggt
      241 cccctccagc ttgagactgc tcctcttatt tttgtgcatc tgccagctta acgcctgcaa
      301 ggcccaggag agtccggaaa gtccgcaagt gacgccgcct ctaaagaagc cgactcccat
      361 caagcgtgtc aaagtggagt tgaccgatag caatccgatt tatgtgagtt atccgcaagg
      421 cggaacaacc gccatcacgg caagcagcag cttcatcata ggctcagttt tgccgggagg
      481 cagtttgagc atcaacggta aaccggtaag cctcaataaa gacggtttct ttgcccagac
      541 cgtgcccctg accaggggcg tcaatcgctt ccaactgctc tacaccaggg atggtgccca
      601 aactccttat tcggcttttg ttcaattagt aagagaaaag ctgagagagc cactttctcc
      661 ctcctccctg gtcatctccg gcgagggcgt cgagcccggt gacgaccggg ccctcaccgc
      721 cggcgatatc attgaatttt cctgccatgc cacacccggg gcagcagtct atgtggagtt
      781 ggcaggtaag aagattgaga tggccagcct ggcaaacttg agagccagag cgaaggagcg
      841 cactggtccg ggcatcaacc ggggtctcga agccgcctac ggacagatct ttcaacggtt
      901 cccccagcac tcccccgatc tatatctggg gctttatcgt atcgaaaacg gcgatttttt
      961 caccaactgt catcctcggt ttgtcctcac caaaggcggt aagaacacca gtctcactct
     1021 cggcaccaat atatcggtgg tcaagcaacc acttatggcc cataccattc acgacgatac
     1081 cattgtgaga gtggcaccgg aactggcgcg tctcaccccg ttgccggcag gagtaagact
     1141 gatcacggac ggctatcaga aagacaacat tcgctgcctt tacaaagcag gcaagcatgt
     1201 ctggataaaa agagaagact tgcaattcga gccgcccggt gctcccgcac cacgggcggt
     1261 gtgccgttcc gtgcaggtag agaaagacga ctggggcgaa atagtttcaa ttcccatgag
     1321 ccagaggcta cccttcctga ttgagcaggc agtaagtgcc ggctcaaaca agctgcaagt
     1381 caaaatttac ggggcgcagt cagatacgga ctgggtttat cagtcgccgg ccgacggtga
     1441 agcaagattg attgaagatg tggcctggaa acaaccggac gacggcgtct atgaaatcga
     1501 catcgccctc aagccgggaa ggcagtgggg ctacttcgcc gaatacaaag aaaacaatct
     1561 caatttacac ataaagtatc cgccccgtct ggtaagcacc gcagaaaaag gacgttcgga
     1621 gagaccgctt gagggtcttg tgatttgcgt cgatcccggt catggcggtc ttgaaagcgg
     1681 cgcactcgga ccgtccggtt tgtgcgaagc cgaggtcaat ctggctatcg ccaagaaatt
     1741 caaagcggcc ctggagaaag aaggagccac cgtcttcatg acaagggaag aagacatcga
     1801 tgtctccctc aacgacagag tagcctttgc cagggataag aaagtggact tacttatctc
     1861 ggtgcataac aacgcgctgc cggatggacg agatcccttg aaggagcacg gcacttccac
     1921 ctaccgctac catccccaat ccatagagct ggcccgctgc ctgaaagact ctatggtcaa
     1981 ggaactggag ttgcctgatt taggcgcccg ctatcaaaac ctggccctgt gccgtcctac
     2041 agccatgcaa gcggtgctgg tggaagtggc ctttatggtc aatcccgatg aatatagcaa
     2101 cctcattaac agccagtggc aaaacaaagc agccagaagc ctggtaaatg gcttactgtc
     2161 atattttgcc cgcaaataaa agcgttgcca ggcagcaatt agaactgttg gcggggcggc
     2221 atcggcccca ggggatccaa tttgaaactt ctttcgagct cttgcctgta agccatatta
     2281 gttagagctt ccaccgagta cagttcgtcg tgccagtaac tatcttcccg atccttaagg
     2341 tcatccagac gcttacggag attggcggga atgagttctt cttgcattaa tcgcctcgaa
     2401 tctagactga aagtgttcct gtattatgcc tggagaatga tttgttgact tatcgcactg
     2461 ccatcttatg tggtgccttt tcgatcaaga aatcattaaa ctaaatctca catgcacagc
     2521 actagtttaa gtccttcatt gggataagca attcttgact tcttcgcata tccagaagaa
     2581 accggcaccg gctgtggctt tcagatttgt ctacttaaaa tcttggctta gactttgaca
     2641 atatatactt ccagcggcag ttccgatcca aatgattcgt tttcggaggt tcagtgaata
     2701 tactcggtgg gaatcactaa cgtcttataa taaagagatg aatttaaagc ttcgactgga
     2761 aaaattatgc gacgcccgcg cctgggtcaa actttccttt gccgacggaa ccgcgcttgt
     2821 ggggcgactt ttacgcatcg ggcaagacta tgtggagctt gagacttttg gtgatccgga
     2881 aaagcgttac tcttccgacc atgccagcga ttactccagg catttgattc ccctggggct
     2941 gataaaactg ctcaccgtcg aatcaccggc ttttgccgaa tacgaaaggc gcaggctcga
     3001 ttatttgtcg aggctcgatt cgcacgatgc gcccgacctg gagcgctagg ctttaggagc
     3061 tactgtcatt cgttctttac ggcgcttagc ccaaccctca attatttcct ggcgcgcccg
     3121 accaacagcg agggcaccgg aaggcacgtc tttggtaaca acggtgcctg cacctacaac
     3181 agcttccgcg cataattcta ccggtgccac cagcaccgaa ttactgccgg tggaggcacc
     3241 gtcaccaact atggtgcgat ctttgcgctt ggtgacatga tcgtaatttg ccgtgatagt
     3301 gccggcgccg atattaacat tgctacccag gctggcatcg ccgatgtagg agagatgccc
     3361 gacattggtg tgatctccca gggttgcttt cttcaattct acgaaatttc caacttttga
     3421 ggcgctgccc acggcattac cttcccgcaa atgggcaaaa ggaccaaccc tggcgcgatc
     3481 accaatgacg gagtcattga cataggaatt gaccaccgag gcaccggccc ctacacgtac
     3541 ccgccccttc atgacagtat agggtccgat cagacagtcg gcgccgattt cgatgtcgcc
     3601 ggtaagaatg cagccgggca taatggtggt ctcctgcccg atttttacct ccggacaaat
     3661 ccaggtactg agcggatcga cgatggtgac gccgctctcc agtgccagcc tgcttacggt
     3721 gatatcgcgc aagtgcctgg ctgcctccga aagttccaga cgcgagttga tgccggccat
     3781 ctcccgccag tcggtaagag ccacagccgc agcgcctgct cccgatttgt aggcccaacc
     3841 aaccaggtcg gtaaggtaat actctttctg cttgttttcg caagtcaacg actgcagacc
     3901 gtctttcacc gcaggccagc tcaaacagta gatgccagta tttacctcac aaatagcctt
     3961 ttcggccggg ctggcatcct tatcttccac aactgccctg actttaccgg cttcatccct
     4021 aacgatgcgg ccgtagttct tggggtcttc tacgatagcc gtcagggctg taacggctgc
     4081 accggactgc tcatgactca aaagcaagtg cttgagcgtg gcactttgta tg
//
LOCUS       DAOQNZ010000149         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9771_length_4118_cov_4.127984, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000149 DAOQNZ010000000
VERSION     DAOQNZ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9771_length_4118_cov_4.127984"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /gene="aspS"
                     /locus_tag="PKW73_03830"
     CDS             <1..1126
                     /gene="aspS"
                     /locus_tag="PKW73_03830"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HNA72439.1"
                     /translation="KIKDILPETPVKVWGTVQAQPNPEVHLKALEILAVVSPAPVEVG
                     KNAKIEKLSAGALFDYRPLTLRNEKVRAIFKIEAEILKAFREFLGKSGFTEIHSPKIV
                     ATGTEGGANLFSLDYFSRPAYLAQSPQFYKQMLVAVFERVFEVGPVYRAEEHETTRHL
                     NEYISLDFEMGFISGIGELIALQQELLKHIFAHLKEHCAFELGLYQAVLPVIEAPIPQ
                     LELAEAVSILKEKYKWQSLSSKDLDAEGEKLICRHIFEETGMELVYITNYPHVIRPFY
                     AMKEKGGSGSGSFDLLFRGLEITTGGQRIHQYGELKASMEERGLEPEQFNDYLMAFKH
                     GMPPHGGLAIGLERLTKQLLALASVKQAALFPRDRNRLTP"
     gene            complement(1116..2039)
                     /locus_tag="PKW73_03835"
     CDS             complement(1116..2039)
                     /locus_tag="PKW73_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72440.1"
                     /translation="MADVTFVTYDGLPLGDPDDQLTLDILSESGLDCATTDWFSGAQA
                     FKESKITVLRSCWNYHMHLPRFRNFLEEVAACSHLLNPLAMVQDNLKKTYLCELARSG
                     LPVCPTAVVEKREELSPELLRSRLALLFEEGFAGAGMFIVKPAVGLATWGVKKVDLDG
                     ADGGAALKTMLAHIQYLREQSPDGDVLLQPYLPQVEKERERALVFIDGEYSHAVSKSA
                     FQHLAVAGQAGESPLQAEPAEIDLARRVLATLPSPPLYARVDLVPASAPGGALLLELE
                     LTEPSLFLSMKEGSAERFARAIQKRLHGLRG"
     gene            2510..2767
                     /locus_tag="PKW73_03840"
     CDS             2510..2767
                     /locus_tag="PKW73_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72441.1"
                     /translation="MRIELVYSPGCSDYKSALHKLEYIIAEERLPLPVELVEDSICKT
                     PSVRIDGEIVEKGRPASCLEPIRDILRRKWHDLTLAPLGCP"
     gene            2909..>4118
                     /locus_tag="PKW73_03845"
     CDS             2909..>4118
                     /locus_tag="PKW73_03845"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA72442.1"
                     /translation="MLFNSLNYALFLPIVFALFWLSRDKWRVWILLLASYIFYMSWKP
                     VFILLILGLTIANYYLGFAIHKSQEPGRKKTWLTIAITGNLLSLAFFKYAYFFNDCLS
                     SLLKATGGAATHLPFDIILPLGISFFVFEFIHYVVDVYRGHEPIKSFPAFALFASFFP
                     TQIAGPIKRFQDFIPQFLAPAKLTVASFDSGLSLILLGLCKKVLIADNLSFFVQGGFS
                     KPELFSGLDLWLFAYAFAFQIYFDFSGYTDVARGSAMLFGYKVPINFNLPYMASNISE
                     FWHRWHISLSTWLRDYLFIPLGGSRGSKWLNYRNLIITMTLGGLWHGAALHFLVWGLF
                     HGLLLAAHKLLQPLKETMPALKKAIDSPAGRVASTLLTFHMVCIGWVFFRAETNSLAL
                     TMLARMLSLSP"
BASE COUNT          958 a         1200 c         1024 g          936 t
ORIGIN      
        1 gaaaatcaaa gatatcttgc cggagacgcc ggtaaaagtc tggggcacgg tgcaagctca
       61 acccaatccg gaagtacatc taaaggccct ggaaatactg gccgtggtaa gccccgcgcc
      121 ggtggaagtg ggcaagaacg ccaaaataga gaagctttcg gccggggcac tcttcgacta
      181 tcgccctctc actttgcgca atgagaaggt aagggccatc tttaagatag aggcggaaat
      241 cctaaaagcc ttccgggagt ttctcggaaa atcaggcttc acggaaattc actcgcccaa
      301 gattgtcgcc accggcaccg aaggcggtgc caatctgttc tcgctcgact acttctccag
      361 accggcctat ctagcccaaa gtccacagtt ctataagcag atgttggtag ccgtcttcga
      421 gagagtcttc gaagtcggac cggtctatcg cgccgaggaa catgagacca ccaggcacct
      481 gaacgaatat atcagcctgg actttgagat gggctttata agcggcattg gcgagcttat
      541 cgccttgcag caagaattgc tcaagcatat ttttgcccac ctgaaagagc actgcgcctt
      601 cgaactagga ctctatcagg ccgtcctacc ggtcatagaa gcgccgattc cacaattgga
      661 actggccgag gccgtctcca ttttgaaaga gaagtacaaa tggcagagcc tctcaagcaa
      721 agacctcgac gccgagggag aaaaactcat atgccggcac atcttcgagg agacaggcat
      781 ggagctggtt tacataacca actatcccca tgtgattcgt cccttctacg ccatgaaaga
      841 aaaaggcggc tccggctccg gcagtttcga cctgctcttt cgcgggctgg aaatcacaac
      901 aggaggacag cgcattcacc agtacggcga actgaaagct tctatggagg aaagaggtct
      961 ggaaccggag caattcaatg actatctaat ggccttcaag cacggtatgc cgccccatgg
     1021 cggtctggcc atcgggctgg agcgcctcac caaacaactg ttggcactgg cttcggtgaa
     1081 gcaggcagcc ctctttccca gggaccgcaa tcgcctcacc ccctgaggcc gtgcagtcgc
     1141 ttctggattg cccgggcgaa gcgctcggcc gagccttctt tcatcgagag gaagagagaa
     1201 ggctcggtca gctccagttc aagcaggagc gcaccaccag gggcggaagc cggcaccaga
     1261 tccacccggg cataaagcgg cggcgaaggc aaagtggcaa gtacgcgccg ggccagatct
     1321 atctccgccg gttcggcctg cagtgggctt tctccggcct gtccggccac agctagatgc
     1381 tgaaaggccg atttggacac ggcatgggaa tattcaccat caatgaaaac cagggcgcgt
     1441 tcgcgctcct tctccacctg aggcaaatag ggttgcaaga gcacgtctcc gtccggagat
     1501 tgctcacgca aatactgtat atgggcaagc atggtcttca atgcagcgcc accatcggcg
     1561 ccatcaaggt ctactttctt cacaccccag gtagccaggc ccactgccgg tttaacaata
     1621 aacatcccgg caccggcaaa gccctcttca aacagcagtg ctagccgaga acgcaagagc
     1681 tcggggctca gctcttctcg cttctccact accgccgtcg gacagacagg caggccgcta
     1741 cgggcaagtt cgcatagata ggtcttcttg aggttgtcct gcaccattgc cagggggtta
     1801 aggagatggg agcaagccgc cacttcctcc agaaaattgc ggaagcgcgg caaatgcatg
     1861 tggtagttcc agcaggagcg caacaccgta atcttgcttt ccttgaaagc ctgagcgccg
     1921 ctaaaccaat cggttgtggc gcaatcaagc ccgctctcgc tcaaaatatc gagggtcagt
     1981 tggtcgtcgg gatcaccaag tggtagcccg tcgtaagtga cgaaagttac atctgccata
     2041 ggtttcgaag attctcgcag agaccgaggt ctgctcatga gaccacagac cgaagggcac
     2101 cggagcaagg ttaagcagat atcaggtgca tagcgacaag attaactttg ctttcacttg
     2161 gtttagcgta tacttctgtt aagggaagcg ttaagctttt cgtcacaatt gtcgaccacc
     2221 cctaagagac ttgctttcaa cccagggcaa cctgtctgta gtaagtttaa aaccgagtgt
     2281 acgacaacgg cgtcacttca agacgaagac ttaatctcgc ctcccgggca ttcttcggta
     2341 agcaacctcc cctcctgcag ccattcagct gaacaggaac ctcagtattg gtccgatcct
     2401 cataatttgc tcagcttaag ggcagcccag gcaaccatcc cagtaactca atcccggggt
     2461 caatttcgtt cggtcctttt tcattccttt ctcaggagga actatcttaa tgcgtattga
     2521 actcgtctac tcgccaggct gcagcgacta caaaagtgca ctgcacaaac ttgaatacat
     2581 catcgcggaa gagagactgc ccttaccggt ggagctagtt gaagattcca tctgcaagac
     2641 accctcggtg cgaatcgacg gcgaaatagt tgaaaaaggt cgcccggctt cctgcctcga
     2701 gcctattcgc gacatattga ggcgcaagtg gcacgacctc accctcgccc ccctgggctg
     2761 cccctaaatc caccaggtcg acaccataag atccgccaag agacccgccc cggtgggtct
     2821 ctttatgtgg caggcttaag gaccagaggg gcgtcaggag ctattattga ctacctgaaa
     2881 cccgcaagaa atagccgcca caaagcgaat gctttttaat tccctcaatt acgccctttt
     2941 cctgcccata gtctttgctc tcttctggct cagccgtgac aagtggagag tctggatcct
     3001 gcttctggcc agctacattt tctacatgag ctggaagccg gttttcatct tgctcatttt
     3061 agggctgacc atagccaact actatctggg ctttgccatt cacaaaagtc aggagcccgg
     3121 gcgcaaaaaa acctggctga ccatagctat caccggcaac cttctcagcc tggccttttt
     3181 caaatacgca tactttttca acgactgcct ctccagcctt ctgaaagcca cggggggagc
     3241 cgcgacccat ctgcctttcg acatcatttt acccctgggg atctccttct tcgtcttcga
     3301 attcatccac tatgtggtgg acgtctatcg tggtcatgag cccattaagt ccttcccggc
     3361 ctttgccctt ttcgccagct ttttccccac gcagattgcc ggccccatca agcgctttca
     3421 ggatttcatt ccacagtttc tggccccggc caaacttacc gtagccagct tcgactccgg
     3481 actgagtttg attttgcttg gtctttgcaa gaaggtcttg atcgccgaca acctcagttt
     3541 cttcgtgcag ggcggtttca gcaaaccgga actctttagt ggacttgatc tctggctttt
     3601 tgcctatgcc tttgcctttc aaatttactt cgacttttcc ggctataccg acgtagcaag
     3661 gggctcggcc atgctcttcg gctacaaggt gcccatcaat ttcaacctgc cctatatggc
     3721 cagcaatatt tcggagttct ggcaccgctg gcacatctcc ctctccacct ggctgagaga
     3781 ctatctgttt atccccctgg gcggctccag aggctctaag tggctcaact acaggaatct
     3841 gatcattacc atgaccctgg gcggactctg gcatggtgcg gccctgcatt tcctggtctg
     3901 ggggttattc cacggactat tgcttgcggc ccacaaactc ttgcagcccc tcaaggaaac
     3961 catgccggcc ttgaagaaag ccatagacag ccccgccgga cgagtcgctt caacgcttct
     4021 aacttttcac atggtctgca tcggctgggt ctttttccgg gctgaaacca atagcctggc
     4081 cctgaccatg cttgcacgta tgctcagctt gagccctg
//
LOCUS       DAOQNZ010000150         4111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9804_length_4111_cov_2.977318, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000150 DAOQNZ010000000
VERSION     DAOQNZ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9804_length_4111_cov_2.977318"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PKW73_03850"
     CDS             complement(<1..1280)
                     /locus_tag="PKW73_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72443.1"
                     /translation="MTNLKKQVHDWLMERHPKLVMALMLRPRIQIIRRDNLEEQIGSL
                     RKVRGDKIESQDFLTRSLIRAEDASSEPILDFCKNLYQRLRLLSPVRFKSDQGQVSRH
                     YLDLEEKLLAGKILLNDLGDRISALKMANPKPYRPRGKKGKRKGKRKRVRPPSKIEAA
                     KAAAINAELERLVKFRDFVELATEEIQDKFLAVQSRHIRKIRETDLKRALDLMEQSQS
                     HAFELNKKINIRICELTAQADIPLVLQVPIEELIPTIRRVEVAVRKRELEAYEGHEYA
                     RNAKPGKMGKVLTLLGRLQSTVGETEKQAKEASLRIEKLLLSAKEFLVAEINNYNSEE
                     LAALILQTDKLLASLKTATDGDSVSGKRREDLEKMRTLFDERLQRLTLNAGEDTIVTA
                     TEEVRKAQMQILDAIEFKSRNLSQLAPTEDPEELA"
     gene            1523..2371
                     /locus_tag="PKW73_03855"
     CDS             1523..2371
                     /locus_tag="PKW73_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72444.1"
                     /translation="MEKVLEKFLVVVLLNLLTASFYADPVIASPKKAVGGHTSAAPSL
                     QKRRTLSPAEQQKFLEARMPGVMIGTFRSYASLFFATQDACIGTHDQDVSETSLQSWY
                     PKKELYMPTHAELFDMIARQTGSSFSYEPKSDYWVFHPPPMGLPYKIAKAQGWTEQNR
                     RGYVAYIPSIAPVGMDIYLLGRYSGLTPDKEKLIREYGAKLFSSHIAPHSTVNEMKEV
                     TVDGATALYFSSPAPKPGLLWRQWAFLKDGELYVIVSAYNKEQKEQIERDVDFMVDSF
                     HSCQAR"
     gene            complement(2438..3697)
                     /locus_tag="PKW73_03860"
     CDS             complement(2438..3697)
                     /locus_tag="PKW73_03860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA72445.1"
                     /translation="MFGINYFRSLGLDRQVLILALGRFVSGMGSGLTMFYAPIYFVNT
                     VGIPAASVGVGLGMGGMSGIVGRIAGGSLADHAGCGRKGAVMLACLTLSLGAFCFAAS
                     NNFIVFLVGNILSGLGIGLYWPAAESMVSDLTGEAKLNEAFGLSRMADYSGLGIGVVA
                     GGLVIGTHAAYRLLFLFDALSYLALLAFVIFGTRETLVLTASHQRPSLLSNWHTALKD
                     KKLALYALLNLIFTNNVLQMSSTLPIYISRLPGMAVAGSVAVVFSYYIILVSLLILPI
                     SRKAARAAKTKALMLACLAWLSGQLLTALLPGLCSLLGLGSEQIMTLLFTMGTVSILA
                     FANALYSPAASSLVVELAPANARATYLSVNSLCWGLAGTVGPAVGLTIIDKGARPALL
                     YWFALALLNAISIAGLWALKKLQDKSI"
BASE COUNT          966 a         1011 c         1055 g         1079 t
ORIGIN      
        1 gccagctcct ccggatcttc tgttggcgcc agttgtgata gattgcggct tttgaattca
       61 attgcatcaa gaatttgcat ttgcgctttt ctgacttctt ccgtcgcagt cactatggtg
      121 tcctcgccgg cgttaagagt tagcctttgt agccgctcgt cgaagagggt tctcattttc
      181 tccagatctt ctcttcgttt gccggagacg gagtccccat ctgttgccgt tttcaatgag
      241 gcaagcagtt tgtcagtttg caagataagg gccgccagct cttccgagtt gtagttgtta
      301 atctccgcta ctagaaactc ttttgcggag agcaagagct tttcaattct gaggcttgct
      361 tccttggctt gcttttcagt ttctccgaca gtactttgta gccggccgag cagggtcagc
      421 acctttccca tctttccagg ttttgcattt cttgcgtact catgcccttc ataagcctcc
      481 agctctcgct tcctcactgc cacttcaacg cgccgaattg tgggtatgag ttcttctatc
      541 ggtacttgca gcactaatgg tatgtcggct tgagccgtga gttcacatat tcgtatgttg
      601 attttcttat tgagctcgaa agcatgagat tgagattgtt ccatcagatc cagagctcgc
      661 ttcaaatcgg tctctctgat ttttcgaata tgtctggact ggacagccag aaacttgtcc
      721 tggatttctt ctgtggcgag ttccacaaag tctctgaatt ttaccaagcg ttcgagctcg
      781 gcattgattg ccgcggcttt agccgcttct atcttgcttg gcggacgaac cctcttccgt
      841 ttccctttcc gtttgccttt cttgcctcgt ggcctgtagg gttttgggtt cgccatttta
      901 agcgctgaaa ttctatctcc aaggtcattc agcaagatct taccggctag aagcttttct
      961 tctagatcca gataatgcct ggagacctgc ccctggtctg acttaaagcg taccggcgaa
     1021 aggagcctca gtctttgata aaggtttttg cagaagtcga gaattggctc tgaactggcg
     1081 tcttcagccc gaatcagact tctggtcagg aaatcttggc tttcgatttt atcaccacga
     1141 accttgcgta gcgaacctat ctgctcttcc agattgtccc ggcgaatgat ttgaattcgt
     1201 ggccgaagca ttaacgccat gaccagtttg ggatggcgct ccatgagcca gtcgtgaact
     1261 tgttttttca ggttcgtcat ggcgttaacc cggctcagcg aagttcaata ccaaaaaagg
     1321 tattctaaaa gcgattatat ccgaagggta tcggtctgga ttcttggaga gcttggggaa
     1381 cgctagcctg aagattcaga gccgggacgc ctattgatgg tgcacagtgg caagtgctcc
     1441 gccctcaggc tttcaaaatg gtaagcttgg ggtagccctt gcaattacct cctgaggact
     1501 acgagccatg agaacctctt cattggaaaa ggtcttagaa aaattcctgg tagtggtcct
     1561 tttgaaccta ctaacggctt ctttctatgc agatccggtt attgcaagtc ccaagaaggc
     1621 agtcggtggg catacgtccg cggcgccgtc gctacagaag cggagaacac tttctcctgc
     1681 cgaacagcag aagtttctgg aagctcgtat gccgggcgtc atgataggca catttaggtc
     1741 ttatgcgagt ttatttttcg ccacgcaaga tgcttgcatt ggcacgcacg accaggacgt
     1801 cagcgaaacg tctcttcagt cgtggtatcc caaaaaagaa ttatacatgc cgactcacgc
     1861 agaactattc gacatgattg cacgtcagac cggatcttca ttctcttatg agcctaagtc
     1921 ggactactgg gttttccatc ctcctcctat ggggctgccc tataaaatag cgaaggcgca
     1981 gggctggact gaacagaaca ggcgggggta tgttgcctac attccgtcga ttgcgccagt
     2041 cggcatggac atttacctgc tgggaagata ttccggtttg accccagata aagagaagct
     2101 gattcgcgag tatggtgcaa agcttttctc cagtcacatt gctccgcact ctacagtcaa
     2161 tgaaatgaag gaagtcactg ttgatggtgc gacagccttg tacttttctt cgcctgctcc
     2221 gaaacccggg ctgctctgga ggcagtgggc gttcttgaag gatggagagc tttatgtgat
     2281 tgtcagtgct tacaacaaag aacagaagga gcaaatagaa cgggatgtgg acttcatggt
     2341 tgattcattc cattcatgcc aggctcgcta atgtggagga ccggactagt gccggttctg
     2401 gctgctggtt catgctcttg ggcgttgttg caaatcgcta tatgcttttg tcctgaagct
     2461 tctttagtgc ccagagaccg gcgatagata ttgcattcaa aagagcaagg gcaaaccaat
     2521 aaagtagagc cggtcttgcc cctttgtcta tgatagtcaa acctactgcc ggtcccactg
     2581 tgccggcgag cccccagcag agtgagttta ccgatagata ggtggcacgg gcgttggctg
     2641 gcgctagctc taccaccagc gaggaagcgg ctggactata gagggcatta gcgaaggcca
     2701 ggatagatac cgtccccatg gtaaagagaa gagtcatgat ctgttcggag ccaagtccta
     2761 ataatgagca gagaccaggc agaagggccg tgagcagttg cccgctaagc caggctaagc
     2821 aggctagcat taaggctttt gttttggctg cgcgcgccgc tttcctggaa atgggcagta
     2881 ttaaaagcga taccagaata atatagtaag aaaacaccac cgccacgctg ccggcgaccg
     2941 ccataccagg caggcgcgat atgtatatgg gcagggtcga actcatctgt aagacattat
     3001 ttgtgaagat caaattgagc agagcataga gagccagctt cttgtctttc agggcggtgt
     3061 gccaatttga gagcaggcta ggtctctggt gtgacgcagt taacaccaga gtctcacggg
     3121 tgccaaagat aacaaaagcc agcaaagcca aataggagag agcatcaaat aaaaagagca
     3181 gtcgataggc ggcgtgggtg ccaatcacga gtccaccggc gacaacgccg atgccgagcc
     3241 ctgaatagtc tgccatacga gacaagccga aagcttcatt cagcttggct tcgccggtaa
     3301 ggtccgaaac catagattcg gctgccggcc agtaaagacc gatgcccaga ccgctaagta
     3361 tattgccgac aagaaaaaca ataaagttat ttgaggcggc aaagcaaaag gcacccaagc
     3421 ttagtgtaag acaggcaagc atgacggcgc ctttgcgacc gcatccggca tggtcggcca
     3481 ggctgccccc ggctatgcgc cccactatgc cggacatacc gcccattcct agcccgacgc
     3541 ccacactggc tgccggtata ccgaccgtat tgacgaaata tatgggagca tagaacatgg
     3601 tgagaccgct gcccatgccc gagacaaagc gcccgagagc aagtatgaga acctgccggt
     3661 ctagacctag cgacctgaag taattgatgc cgaacaaact gacttatccc tcgacgaact
     3721 gccgcttggt tttgctgact agtttctcta gctggctgtt ttctgggctt ttgagattgt
     3781 atatgaattg tcatctcttt gggcggcaga aacaacaatg ggcgtttcaa acttggaaat
     3841 cttagaatca tgttctttga tataatctgt tagccgcgct gctttcgagc tgggttacgg
     3901 atcctctcat ctctcttctg ccgggctgct tattctcgat gtggagatat catggctaag
     3961 gacatcggtt ctcgcaggtt cttgtatttg agatgaaaaa ggaaatcagc aaggaggcct
     4021 acgaggctat ggcttttatg ctcgaaactg cacccgttgc agttttgaga gtttcattcg
     4081 aaggacggct gatgaacatc tcaccgcagt t
//
LOCUS       DAOQNZ010000151         4110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9807_length_4110_cov_4.501110, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000151 DAOQNZ010000000
VERSION     DAOQNZ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9807_length_4110_cov_4.501110"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1116)
                     /locus_tag="PKW73_03865"
     CDS             complement(91..1116)
                     /locus_tag="PKW73_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72446.1"
                     /translation="MNSPLPSTESSTGCKLAKPVSGRIAPGISALLLVLAAPFAAESI
                     APIGSALFLGCTWAPICQAADQVKDVFKQADSAEIQGDEAEALKIYTNLIESLQKSDQ
                     NNPKLARAEARIARLHLLNNQYDKATPFCDKLLKTYHERAAEDPELMVDLDDLSNVYL
                     KFKDDKTRDLDCLNRSLKIREFINHRHPHLPENYRLLSACMLNKNDFPSAINWIAKAI
                     AIDRTYPRQKLDRLVHDLTIQGTIHIKAKNFKAAQASFVDSLNVGKQSGCSDWLVGQC
                     YLHLGYCLMMQKRFDEADENYKLSLTKLKDCPAKLQYLKLLLADHMKENELQRNQSRK
                     SNGKGKA"
     gene            complement(1217..2101)
                     /locus_tag="PKW73_03870"
     CDS             complement(1217..2101)
                     /locus_tag="PKW73_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72447.1"
                     /translation="MGLKQSSKIKGRCITMQTAFLAVCLATSLFTSLFTSPFTATDLM
                     QPAAAAPRRSDYLAYDRFRTTSEATPAAVEKYKQALTLYDNQDMDGAIVKFQELLKMA
                     PKCAGAYYSMGASYMWNDDYQKALDAFNKGVALAPTDFELRYRRAFAFISLLKYTQAI
                     EDLDIAIKMRPERTDAIKLRAKCYLGLKKPDKAAENFGLCIKINPNDSEAYISRAQAY
                     LEQKKFQEALKDTTFMTQKVPDEGLGFFLHGKALMGLKRYKEAATAFDRAISVGTEHS
                     RDCLTMKRECHQLLGKSK"
     gene            complement(2105..3043)
                     /gene="rbsK"
                     /locus_tag="PKW73_03875"
     CDS             complement(2105..3043)
                     /gene="rbsK"
                     /locus_tag="PKW73_03875"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNA72448.1"
                     /translation="MSQKVIVVGSLSMDLVLNVPRLPLVGETIKGDSFSNFVGGKGNN
                     QALAAAKLGASVSMLGLLGKDNYGDLVLGNLQQNNVDCRHMLRTDKTGTGIANIWVGP
                     AGENVIVIIPNANGALSPQLVEENKAIFDDAAVLLLQLEIPLETVTRAAHLARECGVK
                     VILNPAPAPADGKLPAELLGCVDIIVPNETEAMLITGINPDTEENALRCAEKLLAMGP
                     GTVVLTLGARGALIVEQSGNHQFISSFQVKVVDSTAAGDAFLGAMAAGLADNLTAGQA
                     VKQGCAAGALACTRAGAAPSLPSKEALSTLLENATG"
     gene            3142..>4110
                     /locus_tag="PKW73_03880"
     CDS             3142..>4110
                     /locus_tag="PKW73_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNA72449.1"
                     /translation="MSQTLTGIIRFHSVGGAEVLQYEQVPVAEPAHGEVRIKILSLGL
                     NRAEVLLRQGLYLEKPVFPSRIGYEACGIVEAIGEGCAAVLAGTGIEIGSRVSTVPCF
                     SQAKYGVYGETAVVPARSLVACPANLSDIEGSSIWMQYLTAYGAIKMLGDVKPKEYVL
                     ITAASSSVGYAAIQLVNREGAIAIATTRSVAKKEALLKAGAAHVIVTETEDLVAEVKR
                     ITAGKGASIVFDAVAGPMMLPLARAAAYEGKIFIYGALSLESTTFPLQLALKRGLSVC
                     GYTMFQVVDDAARFARAKEYVLAALADGSFKPVIDKVFSYAELRQAQ"
BASE COUNT          828 a          983 c         1154 g         1145 t
ORIGIN      
        1 cctgttgggt gggtatcgga cgttcccttg caaccgttcg ctggacgctt tccgccagct
       61 atgtcttcct ctacctaagc cttcctctat ctaagccttc cccttgccgt tgctttttcg
      121 cgactggttt ctttgtaact cgttctcttt catgtgatct gccagaagca acttcaggta
      181 ctgaagtttg gccggacagt cttttagttt agtgagggat agtttgtaat tctcatcagc
      241 ttcgtcgaag cgcttctgca tcatgaggca gtagcccagg tgcagataac attgtccgac
      301 cagccagtcg gagcagccgc tctgcttgcc gacatttagt gagtctacaa atgaggcctg
      361 ggctgccttg aagttcttcg ctttgatatg gatggtgcct tgaatggtca ggtcatgcac
      421 aaggcggtcc agtttctgcc tggggtaggt tctgtcgatg gctatggctt tggctatcca
      481 gttgatggca gagggaaagt catttttgtt tagcatacag gccgagagca gccggtaatt
      541 ctcgggcaag tggggatgcc tgtggttgat gaattcgcgt attttcaagg agcgattcag
      601 gcagtccagg tctcgagttt tgtcatcctt gaatttgaga tagacgtttg ataggtcgtc
      661 gagatctacc atcagttccg ggtcttccgc tgccctttcg tggtaggtct tcagtagttt
      721 gtcacagaag ggcgtcgctt tgtcgtattg attgtttagc agatgcagtc tggcgatgcg
      781 agcttcggcc ctggctagtt tgggattgtt ctggtccgac ttctgaaggc tttcaatgag
      841 attggtatag attttcaatg cctcagcttc atcgccctga atctccgccg agtctgcttg
      901 cttgaagacg tctttcacct ggtctgctgc ctggcagatg ggggcccatg tgcaacctag
      961 aaaaagggct gagccgatgg gcgctatact ttcagccgca aagggcgcgg caagcaccag
     1021 caagagcgcc gaaatgccgg gggctatgcg ccccgagact ggtttggcga gtttgcagcc
     1081 tgttgaactt tcagttgaag gaagcggact gttcaatgta tttggaattg gagtcgacgg
     1141 tttgagtaat cagagacacc tgttgtgctc tacccgatac attgtggggc tgtttccggc
     1201 agtagcgctt tcttacttat ttactcttac ccaataattg gtgacattct cttttcatag
     1261 tcagacagtc ccgactgtgt tcggttccta ctgaaatggc tctgtcaaag gcggtggctg
     1321 cctctttgta gcgctttaag cccatcaggg ccttgccgtg caggaagaag cccagaccct
     1381 catccggcac tttttgagtc ataaaagtcg tatctttcag ggcctcttgg aacttcttct
     1441 gttccaggta ggcctgtgcc ctggatatat aggcttctga atcgttcggg ttgattttta
     1501 tgcacagtcc gaagttttct gctgctttat caggtttctt caaacccaga tagcattttg
     1561 cgcgcagttt gatggcatcg gtgcgctcgg ggcgcatctt tatggcaata tccaggtctt
     1621 cgatggcctg ggtgtatttg agcaggctga taaaggcgaa agctcggcgg tatctcagtt
     1681 cgaagtcggt gggagccagt gctacgcctt tattgaaggc atcaagggcc ttctgatagt
     1741 catcgttcca catgtaggaa gcacccatcg aataataggc accggcgcat tttggcgcca
     1801 ttttcaggag ttcctggaat ttaacaatgg ctccgtccat gtcttgattg tcatagagag
     1861 tgagggcctg tttgtatttt tccactgccg caggagtagc ttcgctggtg gtgcgaaaac
     1921 gatcataggc aaggtagtcg gagcggcgcg gtgcagcggc tgccggctgc atcaaatcgg
     1981 tggctgtaaa tggcgatgtg aatagcgatg tgaatagcga agttgccagg caaacagcca
     2041 gaaaagccgt ctgcatggtt atgcagcggc ccttgatctt gctggattgc tttagaccca
     2101 tagtctaacc cgtggcattt tccaaaaggg tgctcagtgc ttccttggac ggtaggctgg
     2161 gggctgctcc cgctctggtg caggccaggg ctcctgccgc gcagccttgt tttactgcct
     2221 gtcccgccgt gagattatct gccaaaccgg ctgccattgc tcctagaaat gcatctccgg
     2281 cggcggttga atctactact tttacctgaa atgaagagat gaactggtga ttgcctgact
     2341 gttctactat cagagcaccg cgagcgccaa gcgtcaagac cacggtcccc ggtcccatgg
     2401 ctaagagttt ttctgcgcac ctgagcgcat tctcctctgt gtccgggttg atgccggtga
     2461 tgagcattgc ttccgtctca ttggggacga ttatgtccac gcagccaagt aattctgccg
     2521 gaagtttgcc gtctgccggt gccggagccg ggttgagtat tactttaaca ccgcattccc
     2581 gggctagatg tgccgccctg gtcacagttt ctaaaggaat ttccaattgc aaaagcagga
     2641 cggcggcatc gtcgaatatg gctttgttct cttccaccaa ctgggggctg agagcgccgt
     2701 tggcgttagg gatgataacg atgacgttct cgccggccgg tcccacccag atattggcta
     2761 tgccggtgcc ggttttatcg gttctcagca tgtggcggca gtctacgttg ttttgttgaa
     2821 ggttaccgag aacaaggtcg ccgtagttgt ccttgcctag aagcccgagc atagaaacag
     2881 aggctcccag ttttgcggcc gccagggctt gattgttgcc tttgccgccg acgaagtttg
     2941 agaaactgtc gcctttgatt gtttcgccca ccagggggag tctgggtacg ttcaaaacca
     3001 ggtccatgct gaggctgcct actacaatca ctttctggga cattgaggtt actccgacga
     3061 gtctttatac tagtttgggc tgctttccat tgtaattgcg gtagcagccg gtactgcttt
     3121 gacggagata gagtttctat aatgtcccag actttgaccg gaataatccg ctttcattcg
     3181 gtgggaggcg ctgaagtatt gcagtacgag caggttcctg tagcggaacc ggcacatggc
     3241 gaagtgagaa tcaagatact ttccctggga ttgaaccgag ccgaggtctt gctcaggcag
     3301 gggctttatc tggaaaagcc tgtttttcca tcccgcatcg gctatgaggc ttgcggcata
     3361 gtggaagcca ttggagaagg ctgtgccgca gtcttagccg gtaccggtat tgagataggc
     3421 agtagggtaa gtaccgtgcc ctgcttttcc caggctaagt acggcgtata tggggagacc
     3481 gctgtagttc cggctcgctc tcttgttgcc tgcccggcta atttgtcgga tatagaaggt
     3541 tcttccatct ggatgcagta tctcaccgcc tatggtgcaa tcaagatgct cggcgatgtg
     3601 aagcctaaag agtatgttct tattacggca gcttccagct ccgtaggtta tgccgcgatt
     3661 caacttgtga atagagaagg ggcaatagct attgccacca ccaggagtgt agccaaaaaa
     3721 gaggctcttt tgaaagctgg tgcagcacac gtcatagtca cggaaactga agatctggtt
     3781 gcagaggtta agcgcataac cgcaggcaag ggggcttcta ttgtctttga tgcggtagcc
     3841 ggtccgatga tgttgccctt agccagggcg gctgcttatg agggcaagat ttttatttat
     3901 ggtgctcttt cgctagagtc taccaccttt ccccttcaac tggctttgaa gcgcggtttg
     3961 agcgtttgcg gatatacaat gtttcaagtg gtggatgacg cagcgcgctt tgcccgtgca
     4021 aaagagtacg tgcttgcagc tcttgccgac ggcagcttta agccggttat tgataaagtg
     4081 ttttcgtatg ccgagctaag gcaagctcag
//
LOCUS       DAOQNZ010000152         4102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9836_length_4102_cov_4.099580, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000152 DAOQNZ010000000
VERSION     DAOQNZ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9836_length_4102_cov_4.099580"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKW73_03885"
     CDS             <1..356
                     /locus_tag="PKW73_03885"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA72450.1"
                     /translation="KPVELFGDGSTRRDYTFVGDIVSGIESAMSYDFKGYDIINLGRS
                     EPVGLLEMVQALERHLGKKADIVFKPMQVGDVPYTYADISHARQVLGYSPVTSFDEGV
                     RRFVEWFKESRALPV"
     gene            466..539
                     /locus_tag="PKW73_03890"
     tRNA            466..539
                     /locus_tag="PKW73_03890"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Met,seq:cat)
     gene            complement(649..1518)
                     /locus_tag="PKW73_03895"
     CDS             complement(649..1518)
                     /locus_tag="PKW73_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72451.1"
                     /translation="MKTRFYSFRALPNPLAGSDMPTLKGKAGRLALAVSALSLVLQAA
                     PGYCDAPLQGNVQETGAVENQTFQPAVPMLAPVVKEKKKKLEGEAQGSGLSGQAQDQN
                     DGLNRGQAQEDQLDGPMKGQLQEQGGVLKGSASAQDTDLQAQDPDTADQELMVEWDKW
                     HNRLLWSIQSGVQELINSPENVEPRWDAKRGRVVVGPNIPLGTKATFFVRVSNDKRIV
                     KARIVQPSGYPDYDKALLDAIYALDGTSILRFPRNSKRQRVNETATIITSDRGDREFF
                     KFGDVEKYTVPGR"
     gene            1635..3236
                     /gene="tilS"
                     /locus_tag="PKW73_03900"
     CDS             1635..3236
                     /gene="tilS"
                     /locus_tag="PKW73_03900"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNA72452.1"
                     /translation="MSGELLADPVVKRFKKDFCKLLPPQVGTDDRLSLPPLVVALSGG
                     ADSVFLLAALKSLYADAGQEALLTACHVNHNVRAAALDDENFCRELCLRLAVSFRCRV
                     LAPGPGDEARLRKARYQALAEECAEAGAPYLVSGHHLDDQIETVLFRILRGTAAAGAV
                     SMRGRAALPESEGGLNILRPLLGWSKLDILTALEEARLPHVEDESNAEASYSRNFLRH
                     KLLPVIEKRFPAVRQNIEHFRRGLERDEDYFHELVEEARSECFVYCREGVALSRRGYE
                     SLPEAIRSRILVAFLRRCKVEPDYHCLERLQSVLEGRSPRQSIKDGLSAYLLDDGLLY
                     LQPEGQEADAVSESELVRLSGLMAPVLVPLPVLAGGSLTVPVAWLNMAVKVDCLAEAV
                     GSDSPPSALVQYVKLSGGSALHFRPRRPGDRFFTSKERSFKTFLHRQGGRQGQTLKNL
                     MQGGDEPASLAYGAAMRRLFNENGRGLALRLLPVLAAGEQVLWAPGLAVSGLCLGGGV
                     PTHRLELLPLSQHHENIGAGASTTC"
     gene            3382..>4102
                     /locus_tag="PKW73_03905"
     CDS             3382..>4102
                     /locus_tag="PKW73_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA72453.1"
                     /translation="MKKYGTTWAVLLMLLILVIFAVSLASMGKREEQLTYSGFINLIR
                     EGKANEIKKVTIANGEDVIMVQLQNPENERERPVVVPSEAKEDLVKELNKANINFEVK
                     GVDRSAMLFSMVSSFFLPILIVVGFLFMVRSAQSGGNQAMSFGRSRAKLMMDNKVKVC
                     FNDVAGIDEAKQELQEIVDFLKSPEKFQALGARIPRGVLLVGSPGTGKTLLAKAVAGE
                     AGVPFFSISGSDFVEMFVGVGA"
BASE COUNT          801 a         1084 c         1187 g         1030 t
ORIGIN      
        1 gtaagccggt ggaactcttt ggcgacggct ccaccaggcg tgattacacc tttgtcggtg
       61 acattgtcag cggcatcgaa agcgctatga gttatgactt caaaggttac gacattatca
      121 acctgggccg ctcggagccg gtcggtttgt tggagatggt gcaagccctt gagcggcacc
      181 tcggcaagaa agccgatatc gtcttcaaac ccatgcaggt gggtgatgtt ccttatacct
      241 atgctgacat ctcccacgcc cggcaagtgc ttggctacag ccccgtcacc agttttgacg
      301 agggtgtgag gcgctttgtg gaatggttca aggagagccg tgcactcccg gtttgaactt
      361 gtgcctgact tccgattcta tacttttgtt ttgcgatggg gtttgtaggt ggccgtcgcc
      421 ttgacagtca tgcctcgcta tggataatct gaaatccgac tgcatggcac ggtagctcag
      481 tcggctagag cgggcgactc ataaccgcca ggtcgagggt tcgattcccg cctgtgccaa
      541 attttttcat tgcctcaaga ggctatgttt cggtaatttg aaatccaggt agcaagctct
      601 acctggattt tgcttttgcc tggtctgagt caagtgccgc ctcaggtctc aacgaccagg
      661 cactgtgtac ttttcgacgt cgccgaactt gaagaattct ctgtcgcccc ggtcggaggt
      721 gatgatagtg gctgtttcgt ttacgcgctg gcgtttggaa ttgcgaggaa agcgcaaaat
      781 gctcgtgccg tcgagggcgt aaatagcatc cagaagggcc ttatcgtagt ccgggtagcc
      841 tgaaggttgg acgatacgag ctttgacgat gcgcttgtcg ttggaaaccc gcacaaagaa
      901 ggtggctttt gtgcccagcg gaatgtttgg tcccactacc actcgaccac gctttgcatc
      961 ccagcgtggt tcaacgtttt cggggctatt tatcaactcc tgcacacccg attgtataga
     1021 ccagagcagc cggttgtgcc atttgtccca ttccaccatc agctcctgat cggctgtgtc
     1081 cgggtcctgg gcttgcaaat cggtatcttg ggcagatgca gatcctttca aaacgccgcc
     1141 ctgttcttgt aattgcccct tcatggggcc gtccagctgg tcttcctgcg cctgacctcg
     1201 gttcaagccg tcgttctggt cctgggcctg accgctgagg ccgcttccct gagcttcccc
     1261 ttctaatttt ttcttctttt ccttcaccac cggcgcaagc attggcactg ccggttggaa
     1321 ggtttgattt tccacggcgc cggtttcctg cacattgccc tgcagggggg catcgcaata
     1381 accgggagcg gcttgtaaga ccaggctgag agcgctcact gccagagcca ggcgcccggc
     1441 cttgcccttc aaggtcggca tatccgaccc ggcaagcggg ttcggtagcg ctctgaagga
     1501 gtaaaacctc gttttcatgc cctgtatcac caatttgctc cggcttttat ttgtttccaa
     1561 ctggccccga ccatttactg ttatcttaag agcatctttc ctattttcgc ttgatttgag
     1621 agcaatttcc agtgatgagc ggcgaacttc ttgcagaccc ggtagtaaaa cggtttaaaa
     1681 aagatttctg caagctgctt ccgcctcaag tcgggaccga tgaccgtctt tccctgccgc
     1741 ccctggttgt ggctctgtca ggcggtgccg acagcgtttt cttgcttgcc gccctcaaga
     1801 gtttgtatgc cgatgcgggg caggaagcgc ttcttactgc ctgtcatgtc aatcacaatg
     1861 tgagagcggc ggcgttagac gatgagaatt tttgtcgaga actttgcctg cgcctggcag
     1921 tcagctttcg ctgccgcgtt cttgcgcctg ggccgggcga tgaagccagg ctgagaaaag
     1981 ctcgttatca ggccctggcc gaggaatgtg ctgaggcagg cgcaccatat ctggtgagtg
     2041 gtcatcacct cgatgatcaa atcgagacag tgcttttccg gatcttgcgg gggactgccg
     2101 ctgccggggc tgtttccatg cgagggcggg cggctttgcc tgagtctgag ggcgggctga
     2161 atatcttgcg accgctactt ggctggtcca aacttgacat attgactgcc ctggaggagg
     2221 cgcgcttgcc ccatgtggag gatgaaagca atgcggaagc atcttacagc cgcaacttcc
     2281 tccgtcataa gcttttacct gttattgaaa aacgattccc agccgtcagg caaaatatcg
     2341 agcattttcg caggggcctt gaaagggatg aagactactt ccatgagctt gtggaggaag
     2401 cccggagcga atgtttcgtc tattgccggg agggcgtggc cctcagccgc cggggctacg
     2461 aaagcttacc ggaggctatc aggagtcgca ttctggttgc tttcctgcgg cgctgcaaag
     2521 tagagcccga ttatcattgc ctggaacgcc tgcaatcggt tctggaggga cgctcgccgc
     2581 ggcagagcat caaggacggg ctttccgcct accttcttga cgacgggctc ctttacttgc
     2641 aacccgaggg tcaagaggca gatgccgtat cggagtcgga acttgtgcga ttgtccggtt
     2701 taatggcgcc cgttttggtg cccctgccgg tgctggctgg cggcagtctg accgtgcctg
     2761 tggcctggct caatatggcc gtcaaggtgg actgcctggc tgaggcggta gggtctgact
     2821 cgcctccatc ggcactggtt caatatgtga agttgtcggg gggcagtgcc cttcattttc
     2881 gtccccggcg accgggcgat cgctttttta cttcaaaaga acgttctttc aagacctttc
     2941 tgcatcgtca gggcgggcgc caggggcaga ctctcaagaa cctcatgcag ggcggcgacg
     3001 agcccgcttc cctggcttac ggggcggcca tgcggcggct tttcaatgag aatggaaggg
     3061 gcctggccct caggcttttg ccggtactgg ccgccgggga gcaagtgctc tgggcgccgg
     3121 gccttgccgt ttcggggctc tgcctgggcg ggggagttcc cacccatcgg ctggaacttt
     3181 taccactcag tcaacatcat gaaaacattg gagccggggc aagcacaact tgctaatatg
     3241 aaatagtaat gttaccaacg gttagataat ttatcgcttg ccagggtgtc aactctgcga
     3301 tgacggggcg ccacggggct tctcccgccg aaagtgttat aatttggggc tgtttgatat
     3361 ggaattccca aggcggatga aatgaagaag tacggtacaa cctgggccgt tttgctcatg
     3421 ctcttaattc tggtgatatt cgccgtttct ctggcgagta tgggcaagcg ggaagagcaa
     3481 cttacctatt ccgggtttat caacttgatc cgggaaggca aagctaacga gatcaagaag
     3541 gtgactatcg ccaacggcga agatgtcatc atggtgcaac tgcaaaatcc cgaaaacgag
     3601 cgggaaagac ctgtggttgt gcccagtgaa gccaaggaag atctggtcaa ggaactaaac
     3661 aaggccaata tcaattttga ggtcaaaggt gtagaccgct cggccatgct cttcagtatg
     3721 gtcagctcct tcttcctgcc cattttgatt gtggtgggct ttttgttcat ggtgcgctcc
     3781 gcccagtccg gcggcaacca ggccatgagt tttggccgca gccgggccaa gcttatgatg
     3841 gataacaaag taaaagtctg cttcaacgat gtggccggta tcgacgaggc caaacaggaa
     3901 ttgcaggaga tcgtggattt cctcaagagt cctgaaaaat tccaggctct cggtgcccgt
     3961 atcccccggg gcgtgctcct ggtaggttcg cccggtaccg gtaagaccct gcttgccaaa
     4021 gccgtcgccg gcgaggcggg ggtacccttc tttagcattt ccggctctga tttcgtagaa
     4081 atgttcgtcg gtgttggtgc tt
//
LOCUS       DAOQNZ010000153         4090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9889_length_4090_cov_3.481537, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000153 DAOQNZ010000000
VERSION     DAOQNZ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9889_length_4090_cov_3.481537"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKW73_03910"
     CDS             complement(<1..421)
                     /locus_tag="PKW73_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNA72454.1"
                     /translation="MTSDSSRYSLANLSQEVLIREAILDDAQPLSSLYNFYVKSSTAT
                     FAVQEESPEDRRAWLLHHEARKMPVFVADLDGRVLGFASVSPYHDRCAYAGTCELSVY
                     VDPNCLGKGLGKALIDRLMEHCRTRYHAVLALICSEND"
     gene            complement(487..804)
                     /locus_tag="PKW73_03915"
     CDS             complement(487..804)
                     /locus_tag="PKW73_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72455.1"
                     /translation="MDNSFDGKPRASRKSSALKRSDRPSKRAVASPISVNTRLDQGMP
                     LETAEWTRVEQKLVRLLEFVPVEMVFELVQQVAAYGDDQARRGYILGQEDLLQTTNIE
                     EIA"
     gene            1226..2002
                     /locus_tag="PKW73_03920"
     CDS             1226..2002
                     /locus_tag="PKW73_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72456.1"
                     /translation="MTQSKLTEKVPTSNSEPAYSGATARTDNWWVEPLLVAIVFTAFG
                     IYATYRAFENQYFEFGPYLSPFYSPNMKEFFPDLPFSAALLILPFPLAFRATCYYYRK
                     AYYRSYFAHPPGCAVTWFKRKGYTGEFDFPFNLQNLHRYAFYFAVPIVLILWYDALKT
                     LWYDGGFHLSVLTLVMLANCALLSGYTFGCHACRHLVGGHLDSFSCPENKHSYKKWQR
                     VTALNEKHQEWAWTSLISVAITDIYIRMACTNPSLAFQLF"
     gene            2005..3825
                     /locus_tag="PKW73_03925"
     CDS             2005..3825
                     /locus_tag="PKW73_03925"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNA72457.1"
                     /translation="MSQYEIKEHDVLVIGAGGAGLRAAIEASSLGVNVGLVCKSLLGK
                     AHTVMAEGGVAAALANVDAQDGWQTHFRDTMNGGKMLNNWRMAQLHAQEAPERVKELE
                     RWGAVFDRTKDGKILQRAFGGHTWKRLCHVGDRTGLEMIRTLQDRGVHQGIEVYMECT
                     ITKLFKDGDRICGAFGYYREDGRYILFKAKAIVLATGGVGKSFTVTSNSWEYTGDGHA
                     LAYDTGADLIDMEFVQFHPTGMVWPPGVRGILVTEGVRGEGGILRNSLGERFMFKYLP
                     EATRNDYAANEEEAIKWTNAAVAGEKTDARRPPELSTRDNVARAIYTEVKEGRGSPHG
                     GVFLDISYQDSERVKKKLPSMYHQFKDLADVDITAGPMEVGPTMHYIMGGVRVEADTC
                     MTRVPGLFAAGECSGGMHGANRLGGNSLSDLIVFGQRAGAGAASYVKNLTGHPEIDKG
                     QIEEAIEEMEAPFKRSEGVNPYDVAKELNKVMSTYVGIFREEGDLKTGVAKLEELKEM
                     AKKAKATGTKSYNPGWHLCRDLHNMMICSEAIARSALSRKESRGAHSRLDYPNTESEW
                     STCNTAVERSEGGMKLEHTPLPEMPSELKELFKKKEPSHA"
     gene            3818..>4090
                     /locus_tag="PKW73_03930"
     CDS             3818..>4090
                     /locus_tag="PKW73_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNA72458.1"
                     /translation="MPEASTRTATLKVYRGDREKGEFKTYKVPVEAGMVVLDAIHYIQ
                     QNFDPELAVRWNCKAAKCGSCSGEVNGMPSLMCKTRMDKFRPDEEIM"
BASE COUNT         1027 a         1131 c         1061 g          871 t
ORIGIN      
        1 catcattttc cgagcatatg agagccagga ccgcatgata gcgagttcga cagtgttcca
       61 tgaggcgatc gataagggcc tttccgagac ctttgcccag gcagtttgga tctacatata
      121 cggaaagctc acaagtaccg gcataggcgc agcggtcatg atagggagat acggaggcga
      181 aacccagcac ccgaccatcc agatccgcta caaagacagg catcttgcgg gcttcatggt
      241 gcaacaacca ggcccgtcta tcctctggag attcctcttg cacggcaaaa gtggccgtgg
      301 aggacttgac gtaaaagttg tagagcgagc tcaagggttg ggcatcatcc agaatagcct
      361 cccgtataag cacctcctgg ctcaggttcg ccagtgagta ccggcttgaa tcagaggtca
      421 tgagcttctc cttagccacc attaccagtt cccagggaag gtgcaagacc gaagcaatgc
      481 cttagatcag gcaatttcct ctatatttgt agtctggagc agatcttcct gtccaagtat
      541 ataaccccgc ctggcttgat cgtcgccgta agcagcaact tgctggacca attcgaagac
      601 catttccaca ggcacaaatt caagcaagcg gaccagcttc tgctctactc tggtccattc
      661 ggcagtctcc aggggcatgc cttgatccag tcgggtattg actgaaatcg gtgaggccac
      721 ggctcgcttg gacggacggt ccgaacgctt gagggcggag gatttcctac ttgctcttgg
      781 cttgccatca aaagagttgt ccatgactga ttaacgttcc tctactaaga acttccgcac
      841 cgattcaccc ggcaccgtaa acacaactgc tgactgcggc aatttagagg gaaagacagg
      901 aggacaccag cttaagttcc cctgaaagta agccaggagg ggcaatactg cccaagattt
      961 agataattcg attttaattt ttccaattcc ttctcttgtc aacgagattg ccgtccagcg
     1021 gatctaaaaa acacctataa ctagccttaa aagctggtta tcaagacgca gtgacaagcg
     1081 cagtgcagac ataagtcata tgagagaacc atagttcaag cgtctattta gcgtggctat
     1141 aattgcttat gtcagatagt ggaaaactct tgataagcct ttccaggact tagaaagctg
     1201 acagacagga ctaaggacac agaaaatgac ccaatcaaaa ttgacggaga aggtgccgac
     1261 ctcgaacagc gaaccggctt actccggcgc tacggctcgc accgacaact ggtgggttga
     1321 gcctttactc gtagccattg ttttcactgc ctttggtatt tatgcaacat accgggcctt
     1381 tgagaatcag tacttcgagt tcggccccta cctttcaccc ttctattcgc caaatatgaa
     1441 ggaattcttc ccggacctgc cattttccgc ggccctctta attctgcctt tccctctagc
     1501 tttcagggca acttgctact actaccgcaa ggcctactac cgctcctact ttgctcatcc
     1561 gcccggctgc gccgtcacct ggttcaaacg caaaggttat acgggcgaat tcgattttcc
     1621 cttcaacctc cagaatcttc accgttatgc tttctacttc gccgtaccga tcgttttaat
     1681 tctctggtac gacgccctca agactctctg gtatgacggc ggcttccact tgagcgtgct
     1741 gactctggtc atgctggcta actgtgcact cctttccggc tacaccttcg gctgccatgc
     1801 ctgtaggcat ctggtgggcg gtcatctcga tagcttctcc tgcccggaga acaaacacag
     1861 ctacaaaaaa tggcagcggg tcaccgctct caatgagaag caccaggaat gggcctggac
     1921 ttccctcata tcggtagcca tcacggatat ctacattcgc atggcctgta ctaatccctc
     1981 actcgcattc cagttgtttt aatcatgagt caatacgaaa tcaaagaaca tgacgtactc
     2041 gtcataggag ccggcggagc cggtctgaga gcagcaatcg aagcctcgtc cctgggtgtc
     2101 aatgtcggcc tggtgtgcaa gtcgcttctg ggcaaagccc acaccgtcat ggcagaaggc
     2161 ggtgtcgccg cagctctggc taatgtggac gcacaggacg gctggcagac acatttccgc
     2221 gacaccatga atggtggcaa aatgttgaat aactggcgca tggctcagct tcatgcccag
     2281 gaagcaccgg agcgcgtcaa agaacttgag cgctggggcg ctgttttcga ccgcaccaaa
     2341 gacggcaaga ttctgcagcg cgccttcggc ggccatacat ggaagcgact ttgccacgtg
     2401 ggagaccgca ccggtctgga gatgatccgc accctgcaag atcgcggcgt gcaccagggc
     2461 atcgaagttt atatggagtg caccatcacc aagctcttca aagacggcga tcgtatttgc
     2521 ggagccttcg gctattacag agaagatggt cgctacatcc ttttcaaagc caaggccata
     2581 gtgcttgcca ccggcggcgt cggcaaatcc ttcaccgtca cctccaacag ctgggaatac
     2641 accggcgatg gtcatgccct cgcttatgac accggcgccg acttgatcga catggaattc
     2701 gttcaattcc acccaaccgg catggtatgg ccgccgggcg tgcgcggcat tctggtcacg
     2761 gaaggggtga gaggcgaagg cggcatcctg cgcaacagcc tcggtgagcg cttcatgttc
     2821 aaatatttac cggaagccac caggaacgat tacgcggcta acgaagaaga agccatcaag
     2881 tggaccaatg ctgccgtggc cggtgaaaaa accgatgccc gccgtcctcc cgaactgtcc
     2941 accagagaca acgtagccag agccatctac acggaagtga aagaaggcag aggctcgcct
     3001 cacggtggcg tattcctgga catcagctat caggattcgg agcgggtcaa gaaaaaattg
     3061 ccctccatgt atcaccagtt caaagatctg gctgacgtag acatcacagc cggtcctatg
     3121 gaagttggtc cgaccatgca ctacatcatg ggcggtgtgc gcgtggaagc cgatacctgt
     3181 atgacgagag tgcccggact ctttgccgcc ggcgaatgca gcggaggcat gcacggagcc
     3241 aaccgcctcg gcggcaactc gctctccgac ttgatcgtct tcggtcaacg ggccggtgcc
     3301 ggtgcagcct catacgtgaa gaatcttacc ggtcatcccg aaatagacaa aggtcaaatc
     3361 gaggaagcca tcgaggagat ggaagccccc ttcaagcgca gcgaaggcgt caatccttat
     3421 gacgtagcca aagaactgaa caaagttatg tctacctatg tgggcatctt cagagaagag
     3481 ggcgacctga aaaccggtgt ggccaagctg gaagagctga aggaaatggc caagaaagcc
     3541 aaagccaccg gcactaaatc atataacccg ggctggcacc tctgccgcga cctgcacaat
     3601 atgatgatct gctcggaagc tatcgccaga agcgcccttt cccgcaagga gagccgcggc
     3661 gcccatagcc gacttgacta tccaaataca gagagcgagt ggagtacttg caataccgcc
     3721 gtcgaaaggt cggaaggcgg catgaagctc gaacatactc ccttgcctga aatgcccagc
     3781 gaattgaaag agctgtttaa gaagaaggag cccagccatg cctgaagcct caacccgcac
     3841 cgccaccctg aaagtctata gaggcgacag ggagaagggc gagttcaaga cctacaaggt
     3901 tccggtggaa gccggcatgg tagttctgga cgccatccac tacattcagc aaaatttcga
     3961 tccggagctg gctgtacggt ggaactgtaa agctgccaaa tgcggctcct gtagcggtga
     4021 agtgaacggg atgccttcct tgatgtgcaa gaccaggatg gacaagttcc gtcctgatga
     4081 agaaatcatg
//
LOCUS       DAOQNZ010000154         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_9947_length_4077_cov_4.040030, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000154 DAOQNZ010000000
VERSION     DAOQNZ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9947_length_4077_cov_4.040030"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..786)
                     /locus_tag="PKW73_03935"
     CDS             complement(61..786)
                     /locus_tag="PKW73_03935"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNA72459.1"
                     /translation="MTNETILAEGNSTPSITRRLLALMFDSILVSSLTAVTILLLCLY
                     SSNFCFKYHTESLGPLFPISFTVCLLSPFWPWLNLYSCAEVYKMAGASPTHFGHVPEL
                     LLAWIVVLINIFANLAYHTILTSSKMNATVGQRWLGLKVVSVQGGTVSPIVALLRSAI
                     KLAAWTICSFIYWSAATAPFVNNSLEFFSSAPTIWLLSFVLVACLDSLPVFRKAKRLI
                     ESQWQRSTNTETCMVKSTKSMVN"
     gene            1009..1710
                     /locus_tag="PKW73_03940"
     CDS             1009..1710
                     /locus_tag="PKW73_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72460.1"
                     /translation="MELQDFKSKADAAHESSTVSYLYAVVEHSFSAHTVLLVVYEDGT
                     AVAADSKSPTGYSYWQMTESQFEEFKKDNSVLCDLKVKDYGSDISITDLPTTWFYLKD
                     GSGRRQRIRIYGEFSNNQSLMNAQDRRCHCIVPDVLREAFRRSAEVRTSNSRVWAPEN
                     LCFVLKKSASASKASLSHAVRFEPRTVPGPLEDVFTVKKSDLTESEVNRLLTAYLEKR
                     LIAIRDHLYALTLAN"
     gene            complement(1798..2346)
                     /locus_tag="PKW73_03945"
     CDS             complement(1798..2346)
                     /locus_tag="PKW73_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNA72461.1"
                     /translation="MKKRLQTQLCLTLLVIWQPLAGHCADVETRPELKSTSDLPNFHK
                     VHSFLYRSGEPTAKGVQQCHDLGIKTLIDLRATGERTRREAAWGQEFGMKYINLPMSD
                     KAPTKSQVDTFMETVRQAKKDNAPVLVHCAHGSDRTGCLVGIWRVSEDGFTYDQAYKE
                     MRRYYFGPQFKQLSGAVKARAR"
     gene            2561..3196
                     /locus_tag="PKW73_03950"
     CDS             2561..3196
                     /locus_tag="PKW73_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA72462.1"
                     /translation="MDLTDEALIDKYREEQDMALFKILVRRHQGRVFAVAYRLLGSSE
                     EAEEIVQDCFVKVHQNLHKYRAHSTFSSWLLRICQNLCMDALRMRHRRKDIAAISFDP
                     QAQGQEDENFGSGKPVTQVADAGPGPSQVLDQEEQTRIIEESIQRLPENQKIVLVLHD
                     IQGLSYQQIADQVGTSIGTVRSRLHYGRLKLKEILDPYFSPENRTQPLKSR"
     gene            3203..3952
                     /locus_tag="PKW73_03955"
     CDS             3203..3952
                     /locus_tag="PKW73_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNA72463.1"
                     /translation="MEKPSQARKEDCPQIEEELDAYLDMELDDQRAEAIEQHLAVCGD
                     CTGRLEELSALVSKLQALPEPPLPRDFGDLVEARIKATEQAQEIAPAELAAPVQVGTA
                     KVFQFPRPLLVAAAALALVVVGATFFGSPSSKAPIATVPDQSVTQVAEKPALPVNVPK
                     AVEHRQESSPAVAEKAVAPAPAPALKPSDNPHLVAQSQGKSVPSLTSTAPLAVSQPAP
                     ELIAEYDDIVGDGIDDYGISTNEDGLYAIKL"
BASE COUNT          988 a         1010 c         1050 g         1029 t
ORIGIN      
        1 aatcaaaagc gtgcaaagag cgtctcgcca atctcaccgc ttttgttggt gctgtggcta
       61 ctagttgacc attgattttg tgcttttgac catgcaagtt tcagtgttgg tggatctttg
      121 ccattgactc tcgataagtc gcttggcttt gcgaaatacc ggcagactat cgagacatgc
      181 aacgaggaca aaggacagga gccagattgt tggagccgac gaaaagaatt ccagcgaatt
      241 gttcacaaat ggtgcggttg ctgccgacca atatataaag gaacagatgg tccaagcggc
      301 aagcttgata gcgcttctga gtagtgcgac tattggtgag acagtgccac cttgcaccga
      361 cacaactttt aagcccagcc agcgctgacc cactgtggca ttcattttcg aactcgttaa
      421 gatagtgtgg taagcgagat tagcaaagat attgatcaaa acaacaatcc atgccagaag
      481 cagttccgga acgtgtccaa aatgagtggg actcgccccc gccatcttat aaacctctgc
      541 gcaagagtat aaatttagcc atggccaaaa cggtgaaagc aggcagacag tgaaactaat
      601 tgggaataat ggtccaagag attcagtatg atacttaaag cagaaattgg atgagtagag
      661 acacagcaat aggatggtca ctgcagtcag actggatacc agaatcgagt caaacatcaa
      721 cgctagcagc cgcctcgtga tggaaggcgt agagttacct tccgccagta ttgtttcgtt
      781 tgtcatccca gccttttgtg aaataagagt ttaggagcag tttcttgatc ttagcgcttg
      841 atccgcctgg tggaggggaa tttgattaca aagtttgctt cgtcctcaat tgtttgctcc
      901 atagctctgt tttgtcttgc ggtgacgttt ttgcagagtt gtgtgactaa gccggtgtcc
      961 aactggatac ccagaccagt ttttttcgca tattccttgc agcggcagtt ggaattgcaa
     1021 gacttcaaaa gtaaagcaga cgctgcgcat gagtcgagca cagtgagcta tctctatgca
     1081 gttgtggagc attctttctc cgcgcatacg gttcttctag ttgtgtacga ggatggaact
     1141 gccgttgctg cagatagcaa aagcccgacc ggctatagct attggcaaat gaccgaaagt
     1201 caatttgaag agttcaagaa ggataatagt gtactttgcg atctgaaagt aaaggactac
     1261 ggttctgata tttcaatcac tgacttgccg accacttggt tctatcttaa agacggaagt
     1321 ggaagacgcc agagaatacg aatttatggt gaatttagca ataaccaatc cttgatgaat
     1381 gcccaggatc ggcgttgcca ctgcattgtc cccgatgttt tgagagaggc attcagaagg
     1441 tcggcagaag taagaacgtc aaacagcagg gtctgggctc ctgaaaatct ctgttttgtc
     1501 ttaaaaaagt cagccagtgc ttcgaaggca tcattaagtc acgcagttag atttgaacca
     1561 agaactgtgc cggggccgtt ggaagatgtg ttcacagtca agaaatcaga cctgaccgaa
     1621 tctgaagtta acagattgct gactgcttac ctggaaaaac ggctgatagc tattcgtgac
     1681 catttatatg ccctgaccct tgcgaattag gctcaaccgt gcagggtcat gtggcttgaa
     1741 ttgtttgaaa tttgcatttc aaccgcctca tcacccgtcc gagctcgtgc ttggagctta
     1801 ccttgccctg gctttgacgg caccggacag ttgtttgaac tgaggcccga agtagtagcg
     1861 ccgcatttct ttataagcct ggtcgtaagt gaagccgtct tcgctcacgc gccagatgcc
     1921 gactaggcag ccggtgcggt ctgaaccatg ggcgcaatga accagcacgg gagcgttatc
     1981 cttctttgct tgtctgactg tttccataaa tgtatccacc tggctctttg tgggagcttt
     2041 gtcgctcatg ggcaggttta tatacttcat gccgaactcc tgaccccagg cagcctcgcg
     2101 cctggtgcgc tctcctgtgg ctcttaaatc gatcagggtt ttgatgccca ggtcgtggca
     2161 ctgctgtacg ccttttgccg tgggctcacc gctgcggtat agaaaggaat ggactttatg
     2221 aaagttcggc agatcgctgg tacttttcag ctccggcctg gtttccacgt cggcgcagtg
     2281 accggctaag ggctgccaga tgaccagaag agtcaggcaa agctgggttt gcagtctttt
     2341 cttcatggag tgcctcctct tcgttcaatt tcttgctcta atatagtgtg taaatgcaca
     2401 gtccgttttg tctctttcag ccctatgctg ggtagataga acagaagcga aggatgatta
     2461 acaattgtta atttccaaaa cttttccatt ttaaattgaa ctttttgctg cccttcagcg
     2521 tttatatcct tggaccatac cgatgaggat ggagagtacc gtggacctta ctgacgaggc
     2581 cctcatagac aaataccggg aagagcaaga catggctcta ttcaagatcc tggttcggcg
     2641 acatcaaggt cgcgtctttg ccgtggctta ccgtctcctg ggaagttctg aggaagctga
     2701 ggaaatcgtt caggactgtt ttgttaaagt tcatcaaaac ctacataagt accgggctca
     2761 ttccaccttc tcgtcatggc tcctgcggat ttgtcagaac ctttgcatgg atgcattgag
     2821 aatgagacac aggcgaaagg acattgctgc catatctttt gatccacagg cccaggggca
     2881 ggaggatgaa aacttcggct ccggtaaacc tgtcacccag gttgccgatg ccggcccggg
     2941 gccttcccag gttctggatc aagaggaaca gacccgtatc atagaagaat ccattcagcg
     3001 cctgccggaa aatcagaaga tagtcctggt tctgcatgat atccagggcc tctcctacca
     3061 gcagatagcc gaccaggtag gcaccagtat tggtaccgtg cgttctcgcc tccactatgg
     3121 acgcctcaaa ctgaaagaga tcctagatcc ctatttctct cctgaaaacc gcacccagcc
     3181 cctaaaatcc aggtaacgga caatggaaaa gccaagccaa gccagaaaag aagattgccc
     3241 ccagattgag gaggaacttg acgcctatct cgacatggag cttgatgacc agcgagccga
     3301 ggctatagag cagcacctgg ccgtctgtgg tgactgcact ggccgtctgg aagagttgag
     3361 cgctctggtc tccaaactcc aggctttgcc ggaaccgcca ctgcccagag acttcggcga
     3421 tctggtggaa gctcgcatca aagcaacgga gcaggcgcaa gaaattgcgc ctgccgagct
     3481 tgccgcgcct gtgcaagtcg gaacagcaaa ggtttttcag tttcctcgcc cgcttctggt
     3541 ggcggccgcc gctctggctc tggtcgtagt cggcgccacc ttctttggtt ctccttccag
     3601 caaagctcct attgcgaccg tgcccgacca gtcggttacg caagtggccg agaagccggc
     3661 tttgccggtc aatgtgccca aggctgttga gcaccggcaa gagtcgtcgc cggccgttgc
     3721 cgagaaagcc gttgccccgg ccccggctcc tgccctcaag ccgagtgata atcctcatct
     3781 agtggcacag tctcaaggca agtctgttcc gtcgctaact tcgactgccc cccttgccgt
     3841 ctctcagccg gcgccggagt tgatagccga atacgatgat attgtcggtg atggcattga
     3901 tgattatgga atcagtacta acgaggatgg tttgtatgca atcaaactat aagtcgagcc
     3961 agggtaaatt agaggctggt ggccgacgcc ggttattgct cgccgccctg ttttctctca
     4021 tgtttaccag ctatttcagc actgctgcag aagcggtacc gcctgccact ggcactg
//
LOCUS       DAOQNZ010000155         4066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10001_length_4066_cov_3.490900, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000155 DAOQNZ010000000
VERSION     DAOQNZ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10001_length_4066_cov_3.490900"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKW73_03960"
     CDS             <1..789
                     /locus_tag="PKW73_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA72464.1"
                     /translation="DGIRLSRIAVKTTDLRAYKHNDVEQLKAWLSEDQAKDYRLSVIV
                     TDGVFSMEGEYANLADFVDIAKRNDALLFVDESHSTGVLGATGRGTPEHCGVHGKIDV
                     ISGTLGKALGGAAGGFIAGKKVLVDYLRQKSRPYTFSNSLPPAIVCAAIEAINMLEED
                     NSLVEKLHQNTDYFRKEVQRLGFTILPGVHPIVPVMVGEASIAQDMSNLLLDEGVYVR
                     GLWYPVVPKGEARLRVQISAAHEVEDLDRALSAFQKVGKKLGVV"
     gene            complement(833..1609)
                     /locus_tag="PKW73_03965"
     CDS             complement(833..1609)
                     /locus_tag="PKW73_03965"
                     /inference="COORDINATES: protein motif:HMM:NF036806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72465.1"
                     /translation="MHPAEEEKLEREECQWHITTKAQPEDAPQNSEDNDKPFFTLEEE
                     NPLNAIADSIASQEILEPALTNEDMIVMELLKVAPMNFDELLEKTMLDIPKLNLAITD
                     LEMAGLVISLPGGKIKLLEEAGANTNQQSPPAIPKNLSKEPFSYNSEESLTGSIDASL
                     LELLEAARLRFLMLIKTHFQGISRKYLALYLALRNYLNLSAQTSETKEPWSGRQGQSE
                     IIDLCLLSRNKSFQSTRYFVTALQTKFYFQADSKQKTGAA"
     gene            complement(2278..3216)
                     /locus_tag="PKW73_03970"
     CDS             complement(2278..3216)
                     /locus_tag="PKW73_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72466.1"
                     /translation="MSRDSGQVADKRNLHYVFASILLTGILTLGLTGCQTIKLSGSPN
                     AQNPSAPNSTGNNATSVDMSGPWQVSYEVGGEPKSAHMTLNQTGATFQGTGTDDHDSQ
                     NFVIDKGSIAGTSITFHKRYHVDENPNLPPIIYQGTFEMAKTETYSGPYASGTYSLTK
                     GGQTVGGRWDAQKEGLASPTPAQDTPPANTQVNPAAGNKAPHLSGKWDAGYEFEFKTV
                     HSSIYLEQEGQKLVGHGIDKNSKETFTLSGNYKFPNIRFVLKYNAVKGPKGKAKPERK
                     LEFRGTVANVNESEYQGPRLEGKTNGGGAWMAEIVK"
     gene            3509..>4066
                     /locus_tag="PKW73_03975"
     CDS             3509..>4066
                     /locus_tag="PKW73_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72467.1"
                     /translation="MAEDAAKPKDDKVPEKPKTAEAAAANSEKDATAKLQADAGHVPP
                     PSDASKAAGTDNKAASADATKGTDGKTTDGKTTDGKTTDGKTESGEKAKTGDDKAEGH
                     KEGEERTAAELACEMWDCMKKDLLDAKSRVRGLMGFALHPEMAKSGKPLEIAGGKTEL
                     LASSGTLDFSVPAKSLTGASVEGGED"
BASE COUNT          930 a          961 c         1123 g         1052 t
ORIGIN      
        1 gacggtatca gactttctcg catagctgtc aaaacaacag acctgagggc ctacaaacac
       61 aatgatgtcg agcagcttaa ggcttggctt tcagaggatc aggcgaaaga ctacaggttg
      121 tccgtaatcg tcactgacgg tgtgttcagt atggaaggcg agtatgcgaa cctggccgat
      181 ttcgtcgaca ttgctaagcg aaacgatgcc ctgctctttg tcgatgaatc gcactccacg
      241 ggagttctgg gcgccactgg tcgtggtact cccgagcatt gtggtgtgca cggcaagatc
      301 gatgtgattt ccggcactct cggcaaggct cttgggggcg ccgccggcgg cttcatcgct
      361 ggtaagaagg tgcttgtgga ctatttgaga cagaaatcga gaccttacac tttctccaat
      421 tcattgccgc cggcaatcgt ctgtgctgcc attgaagcga tcaatatgct ggaggaggac
      481 aattccctgg ttgagaaact gcaccagaac accgattact tcagaaagga agttcagcgc
      541 ctgggcttca ctatcttgcc tggagtgcat ccaattgttc cggtcatggt gggagaagct
      601 tctattgctc aggatatgtc gaatctgctt cttgatgaag gcgtttatgt gcgcggcctg
      661 tggtaccccg tggttccgaa aggtgaagcg cgcctgcgtg tgcagatatc ggcagcccat
      721 gaagttgaag atctggacag agctctcagt gctttccaaa aggtcggtaa aaagctgggc
      781 gtggtctagt ggtctaaagt tttgttggat gtcttcgagg aaccggtgca gtttatgctg
      841 cgccggtttt ttgttttgag tctgcctgaa aatagaactt tgtctgaagg gctgtaacga
      901 agtatctggt gctttggaag gacttattcc tggataacag gcatagatcg atgatttcgg
      961 actgtccctg cctgccagac cagggttctt tcgtttctga agtctgagct gatagattta
     1021 gatagttccg cagtgccagg tagagggcca ggtatttcct gcttatgcct tgaaagtgag
     1081 ttttaatcag catcaggaag cgcagccggg ctgcttccag caattcaagt aagctagcgt
     1141 cgatacttcc ggtaagagat tcttcagagt tataactgaa tggctctttt gaaaggttct
     1201 ttggaatagc aggtggactt tgctggtttg tgttcgctcc ggcttcttcg agtagcttga
     1261 ttttgccacc gggcagtgaa attacaaggc cggccatttc aagatctgtg attgcaaggt
     1321 ttagtttcgg aatgtccagc attgtttttt ccagcagttc atcaaagttc atgggtgcaa
     1381 ctttgagaag ttccatgact atcatgtctt catttgtcag ggctggttct aagatttcct
     1441 gcgaggcaat gctgtctgcg atggcgttaa gtggattttc ctcctccagt gtgaagaatg
     1501 gtttgtcatt gtcctcggag ttctgaggtg catcttcggg ctgcgcttta gttgtgatat
     1561 gccattggca ttcctctctt tccagctttt cttcttcggc cggatgcaac cagcgcggag
     1621 tcataaggct gcgctttgtg aagatgggtt ggaatagaag tgaaggtagc gaaatcatgt
     1681 cggcagtggc tgtgctcgtc gaggaatcaa gaacttggaa aatggagtga aagattttta
     1741 gagctgacga ttgtgcaatt tcagccaggg tgctgaacca gttggaactg agaatcgctc
     1801 cgtgttcgaa gagccagatc gctgccagcc aggcgcgcag cttcctaacc ctgtgaaaaa
     1861 tggttccgga tgttagccag ctacgactgc ggcagtttag acaccgatag gtccgtagtt
     1921 ctcgttctac ttctacgtcg gagcaattgc atttgtggca gcgcaataga tctagctctc
     1981 tcatcctatt gatgaggaaa tgccagcaag cttcctctgt cgggaagcca tggcagaagg
     2041 cgtcccaggt ctctctggcg tattgtttat gatcttcttc aagaccgctt ggctgcttgt
     2101 ctctatctgt catggtggag ttctccccgg agtctaaatt agacaacgga aaattcagag
     2161 aaaaagttca atcaccaggt ttcgaatcca agttaaaccg atatcggttt agaaaactct
     2221 agccggaagc aaatggatgg tcgtcgaggg tcggctcgtt ccgtagccag attttgacta
     2281 cttgactatc tcggccatcc aggcgccgcc gccattagtt ttgccttcca ggcgcggacc
     2341 ctgatattcg gattcgttga cgttggcaac agtgccgcga aactcgagtt ttctctccgg
     2401 tttggccttc ccttttggtc ctttgacggc attgtatttg agcacaaagc gaatattcgg
     2461 gaacttgtaa ttaccggaga gggtgaaggt ttctttggaa tttttatcga taccgtgtcc
     2521 aaccagcttc tgtccttctt gctccaggta gatgctggag tgcacagtct tgaattcaaa
     2581 ctcataaccg gcgtcccact taccggaaag gtgcggtgcc ttgttaccgg ctgccggatt
     2641 cacctgcgta ttagccgggg gcgtatcctg ggccggggtt ggcgatgcca gtccctcttt
     2701 ctgtgcgtcc cagcgtccgc caacagtttg cccgcccttt gtgaggctat aggtgcctga
     2761 ggcatacgga ccgctgtagg tctcagtctt tgccatttca aaggtgccct ggtagatgat
     2821 gggcggcaaa ttcggattct catccacatg gtagcgctta tgaaaagtaa tggacgttcc
     2881 ggcgatgctt cctttatcta tgacaaagtt ttggctatcg tggtcgtcgg tgccggtgcc
     2941 ctgaaaggtg gcgccggttt gattcaaggt catgtgggct gatttcggtt caccgccgac
     3001 ctcgtaggag acctgccagg gtccgctcat atccacgctg gtggcattgt taccagtgct
     3061 attcggtgct gacgggtttt gagcattagg gctgccggac agcttgatgg tctggcagcc
     3121 ggtcaatccg agggtgagta tgccggtaag gagaattgat gcaaaaacat aatgcaagtt
     3181 gcgtttgtca gccacctgcc ctgaatctcg actcaatctc cagtcccccg cttccgattc
     3241 aatctagtaa atcctattag gaattctata tcctggcggg aaaaatgtct tggtctggtt
     3301 tccctcatcg aatttcggtg attttgctga gagccaggca caaccatggg aaaaatacga
     3361 agaaaagaac atgaattttc gttgacaagc ggtggctctg gcatcagaat agcaacaatt
     3421 actaggcatt tgcccccaag tccagtccgt ttgtcgccta aaaacctaaa acacaagact
     3481 ggtccggtca tcgggagata tctctgcaat ggcagaagac gccgctaaac ctaaagacga
     3541 taaagtgcct gagaagccga agacagcgga agctgccgcc gccaattcgg agaaggatgc
     3601 aaccgccaaa cttcaagcgg atgccggtca tgtgccgccg ccctctgacg ctagcaaggc
     3661 tgcgggcacg gacaataagg cagcatctgc cgatgctaca aagggtaccg acggcaaaac
     3721 caccgacggc aaaaccaccg acggcaaaac taccgatgga aagacagaat ccggcgagaa
     3781 ggcgaaaacc ggcgacgaca aagctgaagg gcataaggaa ggcgaagagc gcaccgccgc
     3841 agagcttgca tgcgagatgt gggactgcat gaagaaggac ttgcttgatg ccaagtctcg
     3901 cgtcaggggg ctgatgggct tcgccctcca tccggagatg gctaaatccg gcaaacctct
     3961 cgaaattgct ggcggtaaga ctgagcttct cgcttctagc ggcactctcg atttcagcgt
     4021 accagctaaa tcgctgaccg gagccagcgt agaagggggg gaagat
//
LOCUS       DAOQNZ010000156         4044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10109_length_4044_cov_3.609927, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000156 DAOQNZ010000000
VERSION     DAOQNZ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10109_length_4044_cov_3.609927"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PKW73_03980"
     CDS             <1..769
                     /locus_tag="PKW73_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA72468.1"
                     /translation="LNAIDTAECYVTSEEMIGNSVSKRRSEYFLFTKCGHADTFYNPA
                     WSKSEIKASVDRSLKRLKTDYVDLLQLHSCSKEILEKGEVVEALQDAQKSGKVRFIGY
                     SGDGQDAHYAVNMGVFDTLQTSVSIFDQEAIELTLPLAIEKNMGVIAKRPIGNAVWRF
                     PSRPDNSYVVEYWERMRALGYDFLDGSAESIGARALRFTYSVAGVHTMIVGTKVPGRW
                     RSNATLLERGPLSEAEMSAIRDQWKQVAKSHNWVGQV"
     gene            840..1373
                     /locus_tag="PKW73_03985"
     CDS             840..1373
                     /locus_tag="PKW73_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNA72469.1"
                     /translation="MSALDLAGHRRGLVRLLQLAYSGEKAAALAYAGHWRSLPQGTAR
                     DTIVRIEKEEVEHRQTVGTMLNKLGEKPAFWREVLMGTIGRLAYIGCFFTGWFLPMYF
                     AGKLEHENVREYDVAAVHAHALGMDELLPALRHMSKTEAEHELFFSEQVAGHWALKPA
                     RQVFGWDPLPVIEQYRQ"
     gene            1408..2205
                     /locus_tag="PKW73_03990"
     CDS             1408..2205
                     /locus_tag="PKW73_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72470.1"
                     /translation="MSFEPPLLLMTSSFLLGLRHGVDWDHLAAIFDIVTSAGCAGRSR
                     RQCLLASLMYALGHGIVVIALGACAICFASVLPTWVDPVMERLVGLTLVVLSIWVLCL
                     GARQCFSNCTEHKALPLSRGAILLSGLNRGYQQLELWLTGSGRFQKVPDTCTDTAGYN
                     NVTAFSVGLIHGIGAETGSQVLLLTAVASASRASGLLMLASFVSGIISCNLIFALASG
                     LGLTSLGKLKSVLVMVSIFTAVFSFLVGISFLSGMGDSLPDLQKLLP"
     gene            2296..3150
                     /gene="hypB"
                     /locus_tag="PKW73_03995"
     CDS             2296..3150
                     /gene="hypB"
                     /locus_tag="PKW73_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNA72471.1"
                     /translation="MCTECGCGLPLPTSINGKQQSTNGGEHSHDAHLHSHDHDHDHVH
                     SHSHSHESGSAHAHTHGPDLISVHKSILSANNLQAERNRGFFRGIGLLALNLLSSPGA
                     GKTALLKRTIQVLGAGSKCGVIVGDLETENDAIRLRESGAPVVQISTGTLCHLDAGMV
                     AAALSELGARSLDVLFIENVGNLVCPATFDLGEDLRVAMLSVTEGEDKPLKYPPMFRF
                     ADAIVLSKIDLAEACEFDRELALANIGAINPGAPVFEVSAKTGHGMDAWCSFLSARRA
                     VGRVTGPA"
     gene            3229..3750
                     /locus_tag="PKW73_04000"
     CDS             3229..3750
                     /locus_tag="PKW73_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72472.1"
                     /translation="MIRKRPGESTPISRLIRARYKGICLCGRRFSVGDRIEFDPLGRL
                     TRCLGCYQKQLRLSRENLTEPVMEDNSAQFARVERLKMIRALPSPLSPSLNDEYSRLL
                     AELNDEQEIAPCTKLFLQSSARCKSKDRRLYAVKLYSARLCVHCRETQVGGSLVLMDF
                     SKMLVHCILCECY"
     gene            complement(3768..>4044)
                     /locus_tag="PKW73_04005"
     CDS             complement(3768..>4044)
                     /locus_tag="PKW73_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNA72473.1"
                     /translation="VAVVHIKVGALSGVVPEALTFAYDIAREGTILADSKLAIQWVPV
                     RIFCQTCNRIVDLPGIQSFACPACGNGSSDLRSGRELDLVCLEVKES"
BASE COUNT          827 a          968 c         1195 g         1054 t
ORIGIN      
        1 gctgaatgcc atcgatactg ccgagtgcta cgttacaagc gaagagatga tcggaaattc
       61 agtctctaaa aggcgaagcg aatatttttt gtttaccaag tgcggtcacg ccgatacttt
      121 ctacaatccg gcctggagca agtccgagat taaggctagt gttgatagaa gtctgaagcg
      181 gctgaagacg gactatgtag accttttgca gctgcatagc tgcagcaagg aaattttaga
      241 aaaaggggaa gtggttgaag ctctgcagga cgcgcagaag tcgggtaaag tgcgtttcat
      301 cggctatagc ggcgatggtc aggatgctca ttatgccgtg aatatgggtg tgttcgacac
      361 tttgcagacc tcggtgtcta tattcgacca ggaggccatc gagctgaccc tgcctctggc
      421 cattgagaag aacatgggcg tgatcgccaa acgccccatc ggcaacgccg tctggcgctt
      481 cccgtcaagg ccggacaaca gttacgttgt ggaatactgg gagcgcatgc gcgccctcgg
      541 ctatgatttc ctggacggta gtgccgagtc tatcggtgct agagcgctca ggtttaccta
      601 cagtgttgcc ggtgttcaca ccatgattgt cggtaccaag gtaccggggc gctggcgttc
      661 gaacgctact ttgcttgaga gaggaccttt gtctgaggct gagatgtcgg ccattcgtga
      721 tcagtggaag caagttgcta aatcacataa ttgggttgga caggtttgag cttttggctt
      781 gcatggggag tcgctacgtt taaaagatgg aagccgatgg aagaaactta ccggagtcat
      841 tgtcggcttt agacctagcc ggtcatcggc gagggttggt gcggcttctg cagcttgctt
      901 atagcggaga aaaggctgcc gccctggcct atgccggaca ctggcgttct ctgccgcagg
      961 gcactgcccg cgatactatt gttcgcatcg agaaggaaga agttgagcac cggcagacgg
     1021 tgggcaccat gctcaataaa ctgggcgaga agccggcgtt ctggcgggaa gtattgatgg
     1081 gtactattgg gcgcctggcc tatattggtt gtttctttac cggttggttt ctgcctatgt
     1141 attttgccgg caagctggaa cacgagaacg tgcgcgagta tgatgtggcg gcagtgcatg
     1201 cccatgccct ggggatggat gagctgttgc cggccctcag gcatatgtcc aagactgagg
     1261 cggagcatga gctgttcttt agcgagcagg tggccggcca ctgggctctt aagccggcca
     1321 ggcaggtctt cggttgggac ccgttgcccg tgattgagca gtatcgccag tgataccagc
     1381 ttccggcgct cgctccgagg tgacccattg agttttgagc ctcccctgct acttatgacc
     1441 agctcgtttc tgcttggctt gcgccacggc gtcgactggg atcatctggc tgctattttc
     1501 gatatcgtga ctagtgccgg ctgtgccggc agaagtcggc gtcagtgttt gctggcctcg
     1561 cttatgtatg cgctcggtca tggaattgtg gtcattgctc tgggcgcctg cgctatttgt
     1621 tttgcttcag tcctgcctac ctgggttgat ccggtgatgg aaaggttggt cggtttaacc
     1681 ctggtggttt tgagcatctg ggttctttgt ctgggcgcaa ggcagtgctt tagtaattgc
     1741 acggagcaca aggcattacc attgagccgt ggcgcaattt tgttgagcgg cctcaatcgc
     1801 ggctatcagc aattggagct atggctgacc ggatcggggc gctttcagaa ggtgcccgat
     1861 acctgtactg ataccgcggg atacaataat gtgacggcat tttcggttgg attaatccac
     1921 ggaataggag ccgagaccgg ttcgcaagtc ttactgttga cagcggtagc aagcgcttct
     1981 cgggcgtccg gattgttgat gctggctagt tttgtgagcg gcatcattag ctgcaatcta
     2041 atatttgccc tggcttccgg cctggggctg acttcgctgg gcaaactaaa gtcggttctt
     2101 gtgatggtca gtatttttac tgctgtcttc agttttctcg tgggcatcag tttcctttcg
     2161 ggaatgggtg atagtctgcc tgatttgcag aagcttttgc cctgagcttc tgagttaatg
     2221 aatttggcca cgggcggtgt catcatatag attgtgtttt gctttcgtat tgatgagcta
     2281 gtcggagaag aaagaatgtg tactgaatgc ggttgcggtt tgcccttgcc caccagcatc
     2341 aatggtaagc agcagtctac aaatggtggg gagcacagtc atgacgctca tcttcatagt
     2401 catgaccacg accatgacca tgtgcacagc catagccaca gccacgagag cggatccgca
     2461 cacgctcata ctcacggacc tgatctgatt tccgtgcaca agagcattct gagcgctaat
     2521 aatttgcagg ctgagcgcaa tcggggcttt ttcaggggta tcggtctact tgcgctcaat
     2581 ttgctttctt cgccgggtgc cggtaagacg gctttgctca agaggacaat tcaggttcta
     2641 ggtgcaggca gtaagtgcgg cgtcattgtg ggtgatttgg aaactgagaa cgatgccata
     2701 cgtttgaggg agagcggcgc cccggtcgtg cagatcagta ccggtaccct ctgtcatctt
     2761 gatgccggca tggtggcggc ggcgctttcc gagctgggtg ctcgctctct ggatgtctta
     2821 tttatcgaaa atgtcggcaa tttggtttgc cccgccactt ttgatctcgg tgaagatctc
     2881 cgggtggcta tgttgtcggt aacagagggt gaggacaagc ctctcaagta tccgcccatg
     2941 tttcgctttg ccgatgccat tgtgctcagc aaaatcgatc ttgccgaggc ctgcgaattt
     3001 gatcgagaac ttgctcttgc caatattggc gccattaatc ccggggctcc cgtctttgaa
     3061 gtctcagcca agaccggtca cggcatggat gcctggtgta gtttcctctc ggctagaaga
     3121 gcggtggggc gggtgaccgg cccggcctag caagactgtt taattgcaca tttatcctgt
     3181 cgatgttttg acggaccatc aaatctggtt cctttaagga ggctggttat gattcgcaag
     3241 cgtcctggtg aaagcacgcc gatttcacgc ctgataaggg cccgctacaa aggaatttgt
     3301 ctatgcgggc gtcgattttc tgtaggggac cggatcgaat ttgatccgct gggcaggctg
     3361 actcgctgcc ttggctgtta tcagaagcag ctcaggctga gccgggagaa tctcaccgag
     3421 ccggtaatgg aggataatag cgcgcagttt gccagggtag agcgcttgaa aatgattaga
     3481 gccctgcctt ctccgctctc tccatcgctt aatgacgagt actcaaggct tctggctgag
     3541 ctgaatgatg agcaggaaat agccccttgt acaaagctat ttttgcagtc atcggcacga
     3601 tgtaaatcaa aagacaggag actttatgct gtgaagctct attccgccag gctttgtgtg
     3661 cattgccggg aaactcaggt tggcggcagt ctggtattga tggatttttc caaaatgctt
     3721 gtgcactgta ttttgtgtga gtgttattag agagaagcgc cctcccgtca gctctctttc
     3781 acttccaggc agacaaggtc cagctcgcga ccgctgcgca aatccgagct gccgttaccg
     3841 caggcggggc aagcgaaact ttgaatccca ggcaggtcga ctatgcggtt gcaggtttgg
     3901 cagaagattc tgaccggtac ccactggata gccagctttg agtcggccag tatagttcct
     3961 tctctggcaa tgtcataggc gaaggtcagg gcttccggta cgacgccgct cagggcgccc
     4021 actttaatgt gcaccactgc cacc
//
LOCUS       DAOQNZ010000157         4041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10119_length_4041_cov_3.967135, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000157 DAOQNZ010000000
VERSION     DAOQNZ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10119_length_4041_cov_3.967135"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKW73_04010"
     CDS             <1..185
                     /locus_tag="PKW73_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72474.1"
                     /translation="AALWLKTEYARAAALCSGICAPPGENFACFVFCITGNQNLSILP
                     FNRQRVCFRKAPQSLN"
     gene            277..984
                     /locus_tag="PKW73_04015"
     CDS             277..984
                     /locus_tag="PKW73_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72475.1"
                     /translation="MCNVCSTVNNPTFTHRQSDLRSKLWLFLKQLALPKVRGKVFGVP
                     LKRSDIDGLMQGAVLTQKWPLGSTEQHALIYKGDIERLAPGQEVLIRINSACYTGDIF
                     HDQSCDCNEQFEVALKMLTAFDGPGLVLYHIEHEGKSHGYFEKLKSFDGEMYPVKGDR
                     RDFRTAVSILKNLGIRRVRVMTNNPEKVGILREYGLEVTGIVPIVSEDPNLRRFYDYK
                     ARVWNHALPRLADEEAA"
     gene            1011..1826
                     /locus_tag="PKW73_04020"
     CDS             1011..1826
                     /locus_tag="PKW73_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNA72476.1"
                     /translation="MSGLIMTGSEQPVQVASIDLDGTLLDRRKRISAENAAAVELLRA
                     AGFEIVLTSGRILQHTLAYYRQLALTGPIVSADGALVRVPGRKPIHDVTLAPDVVFAI
                     LDTARRLKVTAMVFRHDGIRTTSRFAWNDEIDRHRRELPRHYRESKVERVWRTPTHKV
                     LLYATSDELAQLEAALPDFVSQQTHRIRNTATLEFTSPGISKVAGLASMYKPEQVTFF
                     GDGNNDVAVLRWAGLGVAMHHGTEAALAAADLIAPCTDPASNFAAAVELILSI"
     gene            1843..2496
                     /locus_tag="PKW73_04025"
     CDS             1843..2496
                     /locus_tag="PKW73_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA72477.1"
                     /translation="MNHVQKCECFVITGGPSVGKTTLVNALQKRGRQVLEESAAQIIL
                     EGREHPNKERSAFQRAVLRRQLELEQSMFACGGIWYADRGLLDGAVYHQHHDGYVPDS
                     FYELDVSHYKAAFLLEPLSSFEKNGVRPDFEDLEYTEAITPLFGQIYRSRGVPVYFVP
                     SLLSVEERLVFIESTVAKLRASNSVFNFGIAGAGLSLLPTPLAFPNGDFYETLQHVN"
     gene            2471..3088
                     /locus_tag="PKW73_04030"
     CDS             2471..3088
                     /locus_tag="PKW73_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72478.1"
                     /translation="MKLSNTLIEQALTAGHLRISPSPSRARISATSVDVRLDAIIVVV
                     DPVKVGRGLYFDPARESIQAFWERNGKEVDLRKACRQPFADADGKVRKRVGYWLKPGE
                     ICLAFTYEHLELPTRGPVVLQGELWNRSRTARAFLHTHISAPHIKPGTNNKITLEIKN
                     EGPFDVRLTYLAPIAQIGFDEVSGSVRNCCSSFHGQQKPSGLLCR"
     gene            3378..>4041
                     /locus_tag="PKW73_04035"
     CDS             3378..>4041
                     /locus_tag="PKW73_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72479.1"
                     /translation="MNTNKYQNYMNAFADLVAEALIITQACFVPRAQRGAENVESTLQ
                     HLREAYGTRIKSLGTREDNRDKVLENALGQYYGALVTQLIALAQKWKTLNEGGKNVLI
                     DMDEQCNACSLEALTLARNYSDAANAYVALLWQWELNLRSRIELLVKQISSYCRGRNL
                     FYKGNGGREVLGYSYRQNSRVQFTLCFGYHDGVDALSGSLNELLVETEDRQVVRRIAA
                     LTL"
BASE COUNT          887 a         1018 c         1112 g         1024 t
ORIGIN      
        1 ccgcggctct gtggctgaaa actgaatatg cccgtgctgc cgcactttgt tcgggtatat
       61 gtgcgccgcc cggtgagaat tttgcttgct ttgtcttttg cataactggt aaccagaatt
      121 tatcgatcct tccatttaac cgtcaaagag tttgttttag aaaagcgcca cagtctctga
      181 actgaataca aaaaactact tcagttacgt gaagtggttc ttttcgtgcg cgtggacggg
      241 tcgaaatttt ctttattact aactaaggag ttcgtcatgt gtaacgtatg ttcgacagta
      301 aacaacccca ctttcactca ccgacagtcc gatttgaggt ccaaattgtg gctcttcctg
      361 aagcaactgg cacttccgaa agtaagaggc aaagtctttg gtgtaccgct caagcgcagt
      421 gatatcgatg gtctgatgca gggagcggtc ttgactcaga aatggcccct gggctctact
      481 gagcaacacg cccttatcta caagggtgat attgagcggc ttgcaccggg tcaggaagtt
      541 ttgattcgca tcaacagtgc ttgctacacc ggtgacattt ttcacgacca gagttgtgac
      601 tgcaatgagc agttcgaggt ggcgcttaag atgctcaccg ccttcgatgg tcccgggctt
      661 gtgctttatc acattgagca tgaagggaaa tcccacggct atttcgagaa gttgaaaagc
      721 ttcgacggcg aaatgtatcc ggtcaaaggt gatcggcgcg attttcgcac ggctgtgtcc
      781 atcctcaaga acctcggtat tcgcagggtg cgggttatga ccaacaaccc tgagaaggtg
      841 ggaatcttgc gtgagtacgg tctggaagtg accggcatcg tgccgattgt ttctgaagac
      901 cccaacttga ggcgctttta cgactacaaa gcccgtgtct ggaatcatgc tttgcccaga
      961 ctggctgatg aagaagcggc ttgaatccta actttaattg gagaaattcc atgagtggtt
     1021 taattatgac cggctccgag caaccggttc aagttgcatc cattgacctg gatggaactt
     1081 tgctcgaccg tcgaaagcgg attagtgctg agaatgctgc ggctgttgag ttgttgcgcg
     1141 ctgccggttt cgagattgtc ctgaccagcg ggcgcatttt gcagcacaca ctggcttact
     1201 atcgccagct ggcactgacc ggtcctatcg ttagcgccga tggtgccctg gtgcgagtgc
     1261 ctgggcgcaa gccgattcat gatgtgaccc tggcaccgga tgtggtgttt gccatactgg
     1321 atacggcgcg gcgcctcaag gttactgcca tggtatttcg ccatgatggt atccgtacca
     1381 cttcgcgctt tgcctggaat gatgaaattg acaggcatcg tcgcgaactg ccccggcatt
     1441 accgcgagag taaggtggag cgtgtctggc gcacacccac tcataaggtc ttgctttatg
     1501 ctaccagtga tgagctggct caattggaag ccgctctacc agactttgtg agccagcaga
     1561 ctcatcgcat tcgcaacacc gccacccttg agtttacctc ccccggcatc agcaaagtag
     1621 ccgggctggc ttcgatgtat aagccggagc aggtgacctt cttcggagac ggcaataatg
     1681 acgtggcggt cttgcgctgg gccggcttgg gtgtggccat gcatcacggt accgaagcgg
     1741 ctctggcggc agccgacttg attgctcctt gcaccgaccc ggcaagcaat tttgctgcgg
     1801 cagtggagct tattctttct atttgatctg caagaaggtc tcatgaatca tgtacagaaa
     1861 tgtgaatgct ttgtcattac gggcggtcca tcggtaggta aaactactct ggtgaatgct
     1921 cttcaaaaac gcgggcgtca agtcttagag gagagcgccg cccaaataat cctggagggt
     1981 cgggagcatc caaataagga gcgctctgcc tttcagcggg ccgtgctaag gcggcaattg
     2041 gaactggagc aatcgatgtt tgcttgtgga ggcatctggt acgccgaccg tggtttgctt
     2101 gatggggctg tgtatcacca acatcatgat gggtatgtac cggactcttt ctacgagctt
     2161 gatgtctccc actacaaggc cgctttcctg ctggagccgc tttcgtcttt cgagaaaaac
     2221 ggtgtccgac ctgactttga ggacctagag tatacggagg ctatcactcc gctctttggg
     2281 cagatttacc gaagtcgggg cgtgcccgtt tattttgtac cgtccctgtt gtcggtggag
     2341 gagcgcttgg tcttcatcga gagtacggtg gcgaagctca gggcttcaaa ttctgttttc
     2401 aattttggca tagccggcgc cggtctatct ctccttccga cgcccctggc ctttcctaat
     2461 ggtgactttt atgaaactct ccaacacgtt aattgagcaa gctctcactg ccggacattt
     2521 gcgcatcagt ccatctccca gtcgagcgcg aatcagcgca acgtctgtcg atgtaagatt
     2581 ggacgcgatc atcgtcgttg ttgatccggt caaggtcggt cgggggctgt atttcgatcc
     2641 ggcacgtgag tcgatacaag ctttctggga gcggaacggc aaggaagtcg atctcaggaa
     2701 agcttgtcgg cagccgtttg ccgatgccga cggtaaggtg cgcaagcggg tgggatactg
     2761 gctcaagcca ggcgaaatct gtctggcctt tacctacgag catctggaat tgcccaccag
     2821 ggggccggtg gtattacagg gtgagttgtg gaatcgcagt cgcaccgctc gtgctttcct
     2881 gcacacgcat atttctgctc cccacatcaa acccggtact aacaacaaga ttacgctgga
     2941 gatcaagaac gaaggtcctt tcgatgttcg gctgacttac ctggcgccca ttgctcagat
     3001 tggatttgac gaagtaagcg gctccgttcg caattgctgc tccagctttc acgggcagca
     3061 aaaaccttcc ggcttgcttt gccgctagct aagacccgga cctcaacggt tgttttaccc
     3121 ggccccggta aaacaattgc ctcctgggat ttctttcaag agcagtgaaa cgctcgaaat
     3181 tccgctgttg cattctgtca gtccgaacct tgaccggcga ggacggcccg attgcaatga
     3241 ggcttgtggg ctttgcagtt gcggttttga gaaccgccgt ggctgcaagt caatcgacga
     3301 cttttcttcg ccgcgcatga aacccggtga gatttcgtca ggttctctgt ttaaatccct
     3361 tgatcaggaa aaacactatg aacacaaaca aataccaaaa ttacatgaac gcctttgccg
     3421 accttgtggc cgaggctctg ataatcacgc aggcgtgctt tgtccccaga gctcagcgcg
     3481 gtgcagagaa cgtcgaaagt accttgcagc atttgcgaga agcttatggt acccgcatta
     3541 agtcgttggg gaccagggaa gataaccggg ataaagtcct ggaaaatgcc ctcggtcaat
     3601 actacggcgc gctggtcacg caactcattg ctctcgctca gaagtggaaa accctcaatg
     3661 agggcggcaa gaatgtgctt atcgatatgg acgagcagtg caatgcttgc agcctggaag
     3721 ccctcactct cgcccggaat tattccgatg ctgctaatgc gtatgtagcg ttgctctggc
     3781 agtgggagct gaatctgaga agtcgaatag aactgttggt caagcaaatt tcaagttatt
     3841 gtcgggggcg taacttgttt tacaagggta acggcggacg ggaagtgctt ggatattcct
     3901 accgtcaaaa tagccgggta cagttcaccc tttgctttgg ctatcatgac ggcgtggatg
     3961 cgcttagcgg ttctttgaac gagctcctgg ttgagaccga agaccggcag gtcgtgaggc
     4021 gcattgccgc actcaccctg g
//
LOCUS       DAOQNZ010000158         4036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10139_length_4036_cov_3.982416, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000158 DAOQNZ010000000
VERSION     DAOQNZ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10139_length_4036_cov_3.982416"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKW73_04040"
     CDS             complement(<1..721)
                     /locus_tag="PKW73_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72480.1"
                     /translation="MNTGNGNYSEEQAGTEAAAGLTTREPSPGPDNFQAASSSPLDQI
                     RIASPCPMSWDDLERTESEKARFCGQCRKNVYDIAQMSRPEAELFLQQVAVNMPGGTA
                     CLQIFRRADGTILTDDCPVGLKKVRNLWRRVRLTAASVIALLVSALPVQAGDGPGCAA
                     KDGKSAKNQGVGTAAGGDKKLVPLLGSPAPLPVSGSIRGEMTAPVNWEERALAVPELK
                     SLIEKVKGLEVKGAAKDKEKAA"
     gene            complement(863..1552)
                     /locus_tag="PKW73_04045"
     CDS             complement(863..1552)
                     /locus_tag="PKW73_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA72481.1"
                     /translation="MSESKILIVEDEVRIAEILTDYLKQAGFAVEHKERGEGVVEHVK
                     ACPPDLILLDLMLPGVDGLEICKEVRKFSSLPIIMVSARVEELDRLLGLELGADDYIC
                     KPFSPREVVARVKVALRRLKAQVEATKSEEKELFSVDEEAERISVKGTTLDLTRTEFK
                     LLRLLASRPGRVYPRAQLVDLCAQQEGEPAFDRVIDSHIKNLRKKIARVLGDQEVIHS
                     VYGVGYRFEIA"
     gene            complement(1539..2891)
                     /locus_tag="PKW73_04050"
     CDS             complement(1539..2891)
                     /locus_tag="PKW73_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA72482.1"
                     /translation="MAKEELKTGEVNAPQTPSCEHSLAGSIALRGVFFALVLSTLALL
                     SSYWTVKNSFDAYENSRRAELAYRLQASAPSAATQGTLEIALDQNSPAETIASQKFTK
                     EVKIGLTQIFLSLFLAYTAAFYFLSRSLRFRFAQAVLALDRLCRGHNELAFLQAHAGR
                     YQDELSVLERELVRLSEILAAQDRGHRQWVADTSHELRTPIAVLRAQVEAFQDGVQEV
                     TARNLEVLHNEIMALSKLVDDLHWLAKYDVGQIKVSPSLTDVGAVLQDVVEALCERFE
                     GKGLKIENTTGGAGKILANLDINRLRPVFTNLMENSLRYTDRGGILKISAESDGKELS
                     LRFDDSAPGVSDEVLGRMFERFFRAESSRNRTLGGSGLGLAICKNNMQMLGGTIEASH
                     SPLGGVRIELKMPLNLPPDVTGTKVLDNSREATTNTVAAGSEVNQPDRAEDAVQDVRE
                     "
     gene            complement(2901..>4036)
                     /locus_tag="PKW73_04055"
     CDS             complement(2901..>4036)
                     /locus_tag="PKW73_04055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNA72483.1"
                     /translation="IDGRSFRGSGADLHDYHCFGKPVLSPCYGRVVDLFFSLPDNQPG
                     EVNTVNNWGNYVLIELPGGAHVLLAHLQKDSVRVPLHAWVVPGQMLALCGNSGRSPQP
                     HLHMHVQATAKIGANTIPFHLSNVLLAEEGTEARRYALNLCPAENACLTSPKRNVALK
                     KALRLSVGHWLDYEVQTALCGGYKQRLTVNLDLSGQFYLESSGARIGASQPPRLAFTL
                     SDDLLACFGREGGRDALLDAFALAVGLTPLAEGALTWQDKIPQRLLPRQWWLRHLSWL
                     AAACSESHYKRVWDSRERLWMQTGEHRLTILGFLARRVYTEARLSEQGGLISLVLWRE
                     RGGLTFCRQAPAADKQELVLAAALSGYGVREDNGIPAVMALRG"
BASE COUNT          936 a         1205 c         1020 g          875 t
ORIGIN      
        1 ttgccgcctt ctctttgtct ttagcagcgc ctttaacttc cagacccttc actttttcta
       61 tcaaggattt caattcaggc acggccaggg cacgctcttc ccagttgact ggcgccgtca
      121 tttcgccgcg aatggaacca ctaacaggca gtggtgccgg cgaaccgagc aaaggcacca
      181 gctttttatc gccgccggca gcggtgccga caccctgatt cttagcactt ttgccatcct
      241 tagcggcgca tccgggacca tcgccggcct gcaccggcag agcgcttacc agaagggcga
      301 tgaccgatgc agcggtgagc cgcacccggc gccagaggtt gcgcaccttc ttcaaaccca
      361 cgggacagtc atcggtgaga atggtgccgt ccgctctgcg aaatatctgc aggcaggctg
      421 taccgcctgg catattcacg gccacttgct ggaggaaaag ctcagcctcc gggcggctca
      481 tctgagctat gtcataaacg tttttccggc actgaccgca aaagcgggcc ttctcgctct
      541 cggtgcgctc cagatcatcc cagctcatgg gacagggact ggcgattcta atttgatcca
      601 gggggcttga agatgctgcc tgaaaattat ccggacctgg cgagggttcc cgggttgtca
      661 gtccggccgc tgcctcagtg cctgcctgct cttccgaata gttaccattg ccggtgttca
      721 taagccagac ttctccttca gtcacttttc tcgcgcagag aacctcagta cagccaatac
      781 ttcgatattc cgccattagt ataagttacc ccatcgagag gcaaaaaagc acctggaaag
      841 tactgactgt ggccccaggt tttcaggcta tctcgaagcg gtagccgacg ccataaacgg
      901 agtgaattac ctcctggtca ccaagcactc tggctatttt cttgcgcaag tttttaatat
      961 ggctatcgat aacccgatca aaagccggct caccctcttg ttgagcgcac agatctacaa
     1021 gctgagccct cgggtaaacg cgaccgggac ggctggcgag caagcgcaag agcttgaatt
     1081 cggtgcgggt caaatcgaga gtggtaccct tgacgctgat acgttcagct tcttcatcca
     1141 cgctgaaaag ctctttctcc tcacttttgg tcgcttccac ctgagccttg agacgacgca
     1201 aagccacttt cacacgggcc actacctctc tggggctgaa gggcttgcat atgtaatcgt
     1261 cggcaccaag ctctagtcct aaaagccgat ccagctcttc cactctggcg ctgaccatga
     1321 ttatgggcaa agaggaaaac ttgcgcactt ccttgcaaat ttccaaccca tccactcccg
     1381 gcaacatcaa atccagcaag attaggtcag gcgggcaagc tttcacatgc tccaccactc
     1441 cctctccccg ctctttatgc tcaacggcaa agccggcttg cttgagatag tcggtgagaa
     1501 tctcggcaat gcgcacttca tcttctacaa tcaagatttt actctctgac atcttgaaca
     1561 gcgtcctccg cacggtcagg ttgattgact tcgctaccag ctgcaacagt gtttgtcgtt
     1621 gcttcccggc tgttatctaa aaccttggtt ccggttacat ccggcggcaa attgaggggc
     1681 atcttcagtt caattctcac cccgcccagg ggcgaatgag aggcctcgat ggtaccgcct
     1741 aacatctgca tattgttttt gcaaatggcc agccccagac cggagccgcc cagggtacga
     1801 ttgcgggaac tttcagcccg aaagaatcgc tcgaacatac gccccagtac ttcatcggaa
     1861 acaccggggg cactgtcgtc gaagcgcagc gaaagctctt tgccgtcgga ctcggcgcta
     1921 atcttcaaga taccgccccg gtccgtatag cgcagggagt tttccatcag gttggtaaac
     1981 accggtctaa gtctattgat atcaagattg gcgagaatct ttcccgcacc accggtggtg
     2041 ttctcaatct tcagcccctt accttcaaat ctctcgcaca gggcttccac cacatcttgc
     2101 aagacagcgc ccacatcagt caggctgggg cttaccttga tctgtcccac atcatactta
     2161 gccagccagt gcagatcgtc taccagctta ctgagcgcca ttatttcatt atgcaagact
     2221 tccagattgc gagccgttac ttcctgcact ccatcctgaa aggcttccac ctgggctctc
     2281 agcacggcaa taggggtgcg cagttcatgg gaagtatcgg ccacccattg cctgtgaccg
     2341 cgatcttgcg ctgccagtat ttctgaaagc ctgaccaact ctctctccag aaccgagagt
     2401 tcatcctgat agcgcccggc atgggcttgc agaaaggcaa gctcgttatg gccccgacag
     2461 agcctgtcta aggccagaac ggcctgggca aaacgaaaac gcaaactgcg actgaggaaa
     2521 taaaaagcag cggtgtaagc caggaaaaga ctgagaaaaa tttgagtgag accgattttg
     2581 acttccttcg taaatttctg cgaggcaatt gtttccgccg gggagttctg gtcaagcgca
     2641 atttccaaag tcccttgagt ggcagccgaa ggggcactgg cttgaagccg ataggcaagc
     2701 tcagcacgac ggctgttctc atatgcgtca aaggagttct tgacagtcca ataagaagaa
     2761 agcaatgcca gagtagagag caccagagcg aagaaaactc ccctcaaggc aatggaaccc
     2821 gccagagaat gctcgcagga cggggtctgc ggagcattaa cttcacctgt cttcagctct
     2881 tccttcgcca agctagactt ttatcctctg agagccatta ccgccggaat gccattgtct
     2941 tccctcacac catacccgga aagagccgcc gccagcacca actcttgttt atcagccgca
     3001 ggcgcttgcc ggcagaaggt aagaccgccc cgctcacgcc agagcaccag ggatatgaga
     3061 cccccctgct cagaaagacg agcctcggtg tagacgcggc gagccagaaa tcccagaatg
     3121 gtgagccgat gctctccggt ttgcatccag agccgctccc ggctgtccca gacccgtttg
     3181 taatgcgact cgctgcaagc ggcagccaac cagctcagat gcctgagcca ccactgcctg
     3241 ggcaaaagcc tctggggaat cttatcctgc caggtgagag ccccctccgc cagaggggtc
     3301 agccccacag ccagagcaaa ggcatctaga agtgcatccc gtcccccttc ccgtccaaaa
     3361 caagccaaca ggtcatcgct gagagtaaag gcgaggcgag ggggttggga agcgccaatc
     3421 cgggctcctg aactttccag ataaaactga ccgctcagat cgaggttgac ggtcagacgt
     3481 tgtttataac ccccgcaaag agccgtttgc acttcgtagt caagccagtg ccccacggag
     3541 aggcgaagcg ccttcttgag ggcgacattg cgcttaggtg aagtcagaca agcattttcc
     3601 gccggacata aattaagggc gtaacgccgc gcctcagttc cttcctccgc cagcaaaaca
     3661 ttgctcaagt gaaagggtat cgtgttggca ccgatcttgg ccgttgcctg cacatgcatg
     3721 tgcagatggg gctggggaga acgtcctgag ttgccgcaca gggccagcat ctgccccggc
     3781 accacccagg catgcagagg tactctcaca ctgtctttct gcaagtgggc cagcaaaaca
     3841 tgggcgccgc ctggcaattc aatgagcaca taattccccc agttgttgac ggtgttgacc
     3901 tcgccgggtt gattgtcggg caagctgaaa aagagatcca ccactcttcc atagcagggg
     3961 gaaagcaccg gcttgccgaa acaatgataa tcatgcaaat ctgcaccgct gccgcgaaaa
     4021 ctgcgcccgt ctatgg
//
LOCUS       DAOQNZ010000159         4031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10163_length_4031_cov_4.638330, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000159 DAOQNZ010000000
VERSION     DAOQNZ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10163_length_4031_cov_4.638330"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1171
                     /locus_tag="PKW73_04060"
     CDS             92..1171
                     /locus_tag="PKW73_04060"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNA72484.1"
                     /translation="MMGVFHDLESYGHEQISFFHDKESGLKAIIGVHSTVLGPSLGGC
                     RMWKYTDEQAALRDVLRLSRGMTYKAAVAGLNLGGGKAVVMADARTEKTPAMLKAFGK
                     AVEAMGGRYITAEDVGMSVSDIDTIRGYTKYAVGGSNEGGSGDPSVMTAFGVFQGMKA
                     ALKVTGLGESLEGARVAIQGVGNVGYHLASYLSAAGAKLVITDIYPNQIEKVVQEFGA
                     EVVAPDQIYGVDCDIFAPCALGAVLNARTIPQLKCKIVAGSANNQLEADSDGFDLFSR
                     GVVYAPDYAINSGGLINVAAELDGYNHERVMAKVSQVYNTIVNILERSKKEHIPPHQA
                     ADAMAEQRLTEAKKLSAEPVPALAR"
     gene            1283..1570
                     /locus_tag="PKW73_04065"
     CDS             1283..1570
                     /locus_tag="PKW73_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72485.1"
                     /translation="MGFRVGALARRAYKIGDTLSWNAEDGPLRPEQRPSGGNIRTVGY
                     FNCDNPKCTSWQDCFPAVQMALVTIAKDKIETVEAYDPPAQVDQFAIIEMD"
     gene            1613..2449
                     /locus_tag="PKW73_04070"
     CDS             1613..2449
                     /locus_tag="PKW73_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72486.1"
                     /translation="MKQRILPFVLAATLLLSQALPAWAQSPYWFLYMGQSLLYPLTRG
                     LMGSLYYGPYNANPLYSASYFMKRAASNAAQYPYVAPYYSPGSFGPYGYRNAGLPPNY
                     NYGLTNADLQGQGQGQPQNQQSDQSLYSNNSAQAQPLHLPASPYYPPVGAGNVNQPIY
                     SAPPAVSAAGNQAPVMAAGGQDVGLTAPVAAGSGSPVAQGFIDHLVSKYQGDMSKALS
                     NSDTRSWARAMGVVEEGVNDGSYLPADRLEVIGRILKDNTLDPVSKIDTMRILLKKQG
                     GN"
     gene            2774..3400
                     /locus_tag="PKW73_04075"
     CDS             2774..3400
                     /locus_tag="PKW73_04075"
                     /inference="COORDINATES: protein motif:HMM:NF017697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HNA72487.1"
                     /translation="MPSKDLYPDLDTLKARYRRWQHYNIRIFDMLSDVTIVSPHGGFI
                     EAGTSHIAQLVAGRNYNFFDFQGLLKTRPLDLHITSVNFREPLLAEVLGRSRLAVSIH
                     CMGKEAPGEIWVGGLNKKLRDCMAKALREKGFTVRTDLPRYKGVHPRNFVNMPVEHGV
                     QLEIAGDVVDSFFSTPAKFAREVDFAPETTPVLHNFVAACRKALCQCS"
     gene            3707..>4031
                     /locus_tag="PKW73_04080"
     CDS             3707..>4031
                     /locus_tag="PKW73_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72488.1"
                     /translation="MSDYRRIDLLQCHLTINHDIAHLRSNMRLAANLEMELVDFEKQI
                     DEALGTLEPMVQRLASMACLPLDGAPVTDEEFDLILAAAGTIGGLSHCLETTFGVYRQ
                     VQQAIG"
BASE COUNT          916 a         1095 c         1061 g          959 t
ORIGIN      
        1 gtaaaactat caaggttttg ttgctaacta ttaaccccac aacaattccc ctcgaacatc
       61 aagagccgaa acgcaataag gagagccgcg catgatggga gtcttccacg atctggagag
      121 ctatgggcat gaacagattt cttttttcca cgataaagaa tctggcctga aagctattat
      181 cggcgtgcac agcacggtac tgggtccatc cctgggcggc tgtcgtatgt ggaaatatac
      241 cgatgagcaa gctgctcttc gcgatgtctt gcgcctctcg cgcggcatga cttacaaagc
      301 tgctgtggcc ggtctcaacc tgggcggcgg taaagccgtg gttatggctg acgcccgcac
      361 cgagaagacc ccggccatgc tgaaagcgtt cggtaaagcc gtggaagcca tgggtgggcg
      421 ctatattacc gctgaagacg tcggtatgag tgtttccgac atcgacacca tccgcggtta
      481 cacaaagtat gccgtcggtg gttccaatga aggcggtagc ggcgatcctt ccgtgatgac
      541 tgcctttggg gttttccagg gcatgaaagc agcgctcaag gtcaccggtc tgggtgaaag
      601 cctggaaggt gcccgggttg ccattcaagg tgtaggcaat gtgggttatc atctggccag
      661 ctatctatca gccgccggcg ccaaactggt gatcaccgat atttatccca atcagattga
      721 aaaagtagtg caggagttcg gcgctgaagt ggtcgcaccg gaccagattt acggcgttga
      781 ctgtgatatt ttcgccccct gcgctcttgg tgcagtgctc aatgcccgca ccattccgca
      841 attgaagtgc aagatcgtgg ccggctccgc caacaaccag ttggaagccg attcagacgg
      901 ttttgatctg ttcagccgcg gtgtggttta tgctcccgat tatgctatca attcaggcgg
      961 cttgatcaat gtggctgccg agctggacgg ttataatcat gaacgcgtca tggctaaagt
     1021 gtctcaggta tacaacacta tcgtgaatat cctggaacgc tccaagaaag agcacatacc
     1081 gccccatcag gctgctgacg caatggctga gcagaggctg acggaagcaa aaaagttgtc
     1141 ggctgaaccc gtacccgctc ttgctcggta aacagccgta agctaaagct accaagccag
     1201 taagataaga gcacaattga gcttcaacac ggtatacccc acatttcatt gtccgttctg
     1261 caagcagatc gtagactctg gtgtggggtt tcgtgttggt gccctggcca ggcgggctta
     1321 taaaattggc gataccttga gttggaatgc agaggatggg ccgctgcgcc cggagcagcg
     1381 cccctccggc ggcaatattc gcaccgtcgg ctatttcaat tgcgataatc ccaagtgcac
     1441 aagctggcag gattgttttc cggccgtgca gatggccctg gtaacaattg ctaaagacaa
     1501 gatagagact gtggaagcct atgatccacc ggctcaggtg gatcagtttg ccattattga
     1561 aatggactga aaagcgctta tctgttatat attcctctga ggcggtgcca atatgaaaca
     1621 gagaattttg ccttttgtcc tagctgcgac cttgctactt agccaggctc tgccggcttg
     1681 ggcgcagtcg ccctattggt tcttgtacat ggggcagtcg cttctctacc ctctcacccg
     1741 cggtctgatg ggctctttgt actatggtcc ctacaacgcc aatcccctct attccgccag
     1801 ttactttatg aagcgggccg cttccaatgc tgcgcaatac ccatatgtgg cgccctatta
     1861 cagccccggt tcttttggtc cttacggtta tcgcaatgcc ggtctgccgc cgaactacaa
     1921 ttacggtctg accaatgccg atttgcaggg gcaggggcag gggcagccgc agaaccagca
     1981 gtccgatcag tctttgtact ccaataacag tgcccaggcg cagcctttgc acctgcccgc
     2041 cagcccctat tatccccccg tgggagccgg caatgttaat cagcccattt attccgcacc
     2101 accggcagtg agtgccgccg gcaaccaggc gccggttatg gctgccggcg ggcaagacgt
     2161 cggtttgacg gcgcccgtag cggccggctc cggcagtccc gtggctcaag gtttcatcga
     2221 tcatctcgtc agcaaatatc agggcgacat gagtaaagcg ctttccaatt ccgatacacg
     2281 cagttgggcc agggccatgg gtgtggtgga ggaaggagta aatgacggct cctacttgcc
     2341 tgccgatcgc ctggaagtga tcggtagaat cctcaaagac aacaccttag atccggtgtc
     2401 taagattgac accatgcgca ttttgctcaa gaagcagggc ggcaattaag tgacaaggtg
     2461 cggttctctt ggctagccta gcgcacgcag gcagatttta gtaagaaata ctgatggaaa
     2521 cgacctccgg ccaggggaat ttttcggcaa gcttctccat tgcctggttg acagtgctcg
     2581 gctgagggtt tcggttgaac acggctttct ttacgtttcc agaaataaga aaagccactt
     2641 tagtagggtc taaacgaaat caaacttatt tcctcttttc ctaaccggta acttccaaac
     2701 tcaaaaagct agaagagcca atctcttact aaagctctgc cgaatctatt cagacaacca
     2761 tctggagcgc gttatgccgt ctaaagacct gtaccctgat ttagatacgc tgaaagcgcg
     2821 ataccgtcgc tggcagcact acaacatccg catcttcgac atgctgagcg atgtcaccat
     2881 tgtttcgccc catggcggtt tcattgaagc cggtacctcc cacattgctc agcttgtggc
     2941 cggacgaaac tacaacttct tcgacttcca gggcctgctc aaaactcgcc cgctcgattt
     3001 gcacatcact tccgtcaact tccgtgagcc cttgctggct gaagtacttg gtcgttctcg
     3061 cctggcggta tccatccact gcatgggcaa ggaggcgccc ggagaaatct gggtgggcgg
     3121 cctgaacaag aagctgcgtg actgcatggc taaggctttg cgggaaaaag gcttcactgt
     3181 gcgcacggac ctgccccgct acaagggtgt tcacccgcgc aacttcgtta acatgcccgt
     3241 cgaacacggc gtgcagttgg aaattgccgg ggacgttgtg gacagcttct tcagcacacc
     3301 tgcaaagttt gcccgagaag tggattttgc cccggagaca accccggtcc tgcacaactt
     3361 cgtggctgct tgccgcaagg ccctctgtca gtgctcttga aatatgagct agcgctaatt
     3421 tcactagttg atcttgagat taaacaagca tttctagtgt cattagcgaa gctctagtag
     3481 aaatcacatc tttcaacacc ttgtctttta agcttcttca agcttaggac agagttatct
     3541 ctcattttca aatcagaatc aggtcaaaga ctcaagtaca ttgtcggcgc cttagcttag
     3601 cccatttctt ctgactgctg gtcagacgtt gggacagttc tgaattgttg tgagaagtcg
     3661 cagcagcaat gctttcactg taatgcaacc tggaaaggat gtaactatga gcgactatcg
     3721 tcgtatcgac cttttgcagt gccacctcac catcaaccac gacatcgccc acctgcgcag
     3781 caacatgcgt ctggcggcga acctggagat ggaactggtg gacttcgaga agcaaatcga
     3841 tgaggccctg ggcaccctcg agcccatggt ccagcgactg gcttccatgg cctgcctgcc
     3901 cctcgacggt gctcctgtca ccgatgagga attcgacctg attctggcgg ctgcgggcac
     3961 catcggcggt ctcagtcatt gcctcgaaac caccttcggt gtctaccggc aggtgcagca
     4021 ggcaatcggg c
//
LOCUS       DAOQNZ010000160         4028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10177_length_4028_cov_3.840926, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000160 DAOQNZ010000000
VERSION     DAOQNZ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10177_length_4028_cov_3.840926"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKW73_04085"
     CDS             <1..785
                     /locus_tag="PKW73_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72489.1"
                     /translation="MLDFMEQHYLKSFPFKEEDVPAGLDPLSNIACHCQLGTMHQLLK
                     CYGVEEARLAWLREWYLRYKLPDGGLNCDEAAYLKPKPKSSVVSTLPPLEAMLAYGDE
                     LSKEELSFLDDGARYLIERKLFRSATTGAIMDEAWLKLCFPRFYHYDILRGLSFLLKW
                     SRLREQFLPATAIVEAVRLIDDAFPDGLVSPGRQCFAGASSRFYNPETGSWSRQPARS
                     FPLLEEVSREGKVSPYLTELWQEARVNLASLLEAGLISDSES"
     gene            complement(786..1598)
                     /locus_tag="PKW73_04090"
     CDS             complement(786..1598)
                     /locus_tag="PKW73_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72490.1"
                     /translation="MFLLVSALFCSLPLVKAEQLTEREKAERAYKSDDLATLAKSNDI
                     EVRARVAENLNTPEAVLLSLAGSMKLSTYAENDDRDIFFALLKNPSLPPSVFSVLAKK
                     TPLDWDFTDHPRAPLTFILQRLIRDDLYQKGDPDYEEFQALLAMRGKASDGQILDFMS
                     RAKNFSLVKIAARNSATPPASLRALYLRSKADKSVGESIKQYLAENPSLPAEIMEALS
                     HEKENDIRAHLAENSNCPKAVLERLAKDQERYVAEKAIKTYYYKNGGVLLRP"
     gene            1733..2776
                     /locus_tag="PKW73_04095"
     CDS             1733..2776
                     /locus_tag="PKW73_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNA72491.1"
                     /translation="MPFRFFHSDENEKERRRLQDLSIEALSRGDITPDARRRLEAHKN
                     SPFFTTTMSPAGLLLSKQAGYKVLTQVMGSSFIYVNREEARRTYGDYTCEVLTLTNSY
                     EQVRTNALRRLRKEAALLEADGVVDVRVLVKEKEWQEGLLECTISGTAIRVPNKPEFK
                     KPEGSVQRLFNKTKSRPISHPAGGKMQVGADASLDTKANAAPFLSNLTGQEFWQLYQA
                     GYWPTTLVSANCCYFVMADQQARKAQSGFLAGQPNQELECFSKGIYHCRYQVSNKLKR
                     EAKESGGEGIIGVQFDSEIERVTFDKEPNFAFGINVNMSALGTAVRAVPSDSLPKETG
                     SLMVLNLDRKKEK"
     gene            2845..3618
                     /locus_tag="PKW73_04100"
     CDS             2845..3618
                     /locus_tag="PKW73_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNA72492.1"
                     /translation="MSRIKLTSWNVNGIRAVSKKGFFEWFEAHKPDVLGLQETKISAD
                     QLTDELTKRKGYKSYFAHAERRGYSGVGIYTKIEPLTVQEGFGVKRFDDEGRTLVADY
                     GDFLLANIYFPNGAANEDRLAYKMDFYTEFLKWARKQSKAGRKLIVCGDFNTAHKPID
                     LARPKENEKISGFLPEERDWMDKYIKAGNIDTFREFDQSADKYTWWHMRTNARERNVG
                     WRIDYFFANSLLKDNLAGAGILADVHGSDHCPVTLELKI"
     gene            3664..>4028
                     /locus_tag="PKW73_04105"
     CDS             3664..>4028
                     /locus_tag="PKW73_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72493.1"
                     /translation="MNELPAPTTIESGKIWENRSTKEILYGIAKIKKADYAILRIASH
                     DINIDGEILLSKGSTVSGARLILEDGAVEGYPALKALLSVHDGTYSLLDYSQISDQAV
                     HLEQGLKVKINQLLNALPN"
BASE COUNT         1092 a          973 c         1032 g          931 t
ORIGIN      
        1 ggatgctgga ctttatggag cagcattatc taaaaagctt tcccttcaaa gaagaggatg
       61 tgccggcagg tcttgatcct ctgagcaata tcgcctgcca ttgccagctt ggcaccatgc
      121 atcagctcct caagtgctac ggagtagagg aagcaagact ggcctggctg agagaatggt
      181 atctgcgtta caagttaccg gatggcggct tgaactgtga tgaagcagcc taccttaagc
      241 ccaagccgaa gagctcggtt gtatcaactc tgccgcccct ggaagccatg cttgcttatg
      301 gtgacgaatt gagcaaggag gaactatcgt ttctagatga cggtgcacgt tatctgatag
      361 aaaggaagct cttccgctct gccaccaccg gcgccatcat ggatgaagcc tggctaaagc
      421 tctgttttcc tcgcttctac cactatgata ttttgagggg tctttccttc cttctgaaat
      481 ggagccgact gagagagcag tttttacctg ccactgccat agtggaagcg gttcggctaa
      541 ttgatgatgc ttttcctgat ggcctggttt cacccggcag acagtgcttt gccggggcca
      601 gttcgcgctt ctacaatccg gagacaggca gctggagcag acaaccggcc aggtctttcc
      661 ccttgcttga agaagtgagc cgcgaaggca aagtctcacc gtacttgact gaactctggc
      721 aggaagcgag agtaaatctt gcgtctttgc ttgaggccgg actcatctcc gatagcgaaa
      781 gctgactaag gtcgcaagag cacgccgccg tttttatagt agtaagtttt tatggccttc
      841 tcagccacat agcgctcttg atctttcgcc aggcgttcca gcacagcctt gggacagtta
      901 gaattttccg ctagatgagc ccgtatatca ttttcctttt catgactgag tgcttccata
      961 atttctgcgg gcagggacgg gttttcagcc agatattgct ttatgctttc accaacgctt
     1021 ttgtcggctt tgctgcgcaa atagagcgct ctcaaactgg caggcggcgt ggcagagtta
     1081 cgagccgcaa ttttcacaag gctgaaattc ttagctcggc tcataaagtc aagaatctgc
     1141 ccgtcggaag ctttgcctcg catagctaac aaggcctgaa actcttcata gtcaggatca
     1201 cccttttgat ataagtcgtc tcgaatcagg cgctgcaaaa taaaggtaag cggtgcgcgc
     1261 ggatggtcgg taaagtccca atcgagaggg gttttcttgg ctaaaacaga aaagaccgaa
     1321 ggcggcaagc tgggattttt gagcagggcg aagaaaatat cccggtcgtc gttttcggca
     1381 taggtgctca gtttcatgga gccggccaaa ctcagcaata ccgcttcggg cgtgttgaga
     1441 ttttctgcca cgcgggctcg cacctcaata tcattacttt tggctagagt tgccaaatca
     1501 tcacttttgt aggcccgttc ggctttctct cgctccgtca attgctcggc tttgaccaga
     1561 ggcaaagaac agaataaggc ggaaactaac aaaaacatgg taagtcttga tttgcgcgtc
     1621 atcgctaatg ttccccttac tctccagcaa tcttataaac tgtacaatca caggaagaaa
     1681 tttagcaggc aattgaagct aaaattagtc gagtttccag cggagaatat ccttgccgtt
     1741 ccggtttttc catagcgacg aaaatgaaaa ggagcgcagg cgcctgcaag acttgagcat
     1801 cgaggctctg agcaggggcg acatcactcc cgacgccaga agacgactgg aagcgcacaa
     1861 aaatagccct ttctttacaa cgaccatgag cccggccggg cttttattgt caaagcaggc
     1921 aggctacaag gtattgaccc aggtcatggg ctcatctttc atttatgtca acagggagga
     1981 agcccggcgg acttatggtg actatacctg cgaggttttg accctaacga acagttacga
     2041 gcaggtccgc acgaatgcgc tacgcaggct gaggaaggaa gcggcgcttt tagaagctga
     2101 cggtgttgta gatgtaagag ttttggtaaa ggaaaaggaa tggcaggagg ggctgcttga
     2161 atgcaccatt agcggtaccg ccatcagagt tccgaacaaa ccagaattca aaaagcctga
     2221 gggctcagtt caaagactct tcaacaagac aaagagcaga cctataagcc atccggccgg
     2281 tggcaagatg caagtcggag cggatgcatc cctggacaca aaagctaacg cagctccctt
     2341 tctcagcaat ctgaccgggc aagaattctg gcaactttat caagccggct actggccgac
     2401 gacgcttgtt tcagccaact gttgctactt tgtcatggcc gaccagcagg caagaaaagc
     2461 ccagtcagga ttcttagccg gccaacccaa tcaggaactc gaatgcttta gtaaaggcat
     2521 ctaccactgt cgctatcaag taagcaataa acttaagaga gaggctaagg aaagcggggg
     2581 cgaaggcatt atcggcgttc agttcgactc agaaatcgaa agagtcacct tcgacaaaga
     2641 gccgaacttc gccttcggca tcaatgtaaa tatgtcggcc ctgggcacgg ccgtaagagc
     2701 ggttccaagc gatagcttgc caaaagaaac tggttcttta atggttctca atcttgatag
     2761 aaagaaagaa aagtaaaagc aggcagataa aagtctgcac gctctttata ataaccatgg
     2821 ggaaagtcgc ggcaggaaac acaaatgtcc agaatcaagc ttacgtcgtg gaatgtcaac
     2881 ggcatcagag ccgtgagtaa gaaaggcttt tttgaatggt ttgaagcgca caagccggat
     2941 gttcttggac tgcaagagac caaaataagt gccgatcaac tcaccgacga actgaccaag
     3001 cgcaaaggct acaagagcta ttttgcccat gccgagagaa gaggttacag cggcgtaggc
     3061 atctatacaa aaatagaacc actgacggtg caagagggct tcggcgtcaa acgcttcgat
     3121 gatgaggggc gcacactcgt agccgactac ggagacttct tgctcgccaa catttacttc
     3181 ccaaatggtg ccgccaatga agatcgcctc gcttacaaaa tggattttta caccgagttt
     3241 ttgaaatggg ccaggaagca gagcaaggct ggtcgaaagc ttattgtctg tggcgacttt
     3301 aacacggccc acaagcccat agatctggca agaccaaagg aaaatgaaaa gatctcaggc
     3361 tttctaccgg aagagcgcga ctggatggac aaatacataa aagcgggaaa catcgacact
     3421 tttagagagt tcgatcagtc tgcggacaaa tatacatggt ggcatatgcg caccaatgcc
     3481 agggaacgaa atgtgggatg gcgcatagac tatttcttcg ccaatagcct tttgaaagac
     3541 aatctggcag gggcaggcat tctggccgat gtgcacggct ccgatcattg ccctgttacc
     3601 ctggagctta agatctgagg caagcgcttg atattaagcg tgaaataggg gatatttttt
     3661 cctatgaatg aactacctgc gcccaccaca attgaatcag gcaaaatttg ggagaaccgc
     3721 tccaccaagg aaattctcta cggcattgcc aagataaaga aagccgacta cgctattttg
     3781 cgtatcgctt cccatgacat caacatagac ggcgagatct tgctttccaa aggcagtacc
     3841 gtctcaggag cgcggctcat tttggaagac ggagcggtgg aaggctatcc ggccttaaaa
     3901 gcgcttctgt cggtgcacga cggcacctat tcacttctag attacagcca gatcagtgac
     3961 caggcggtgc accttgaaca gggtctgaaa gtgaaaataa accagctcct caatgccctg
     4021 cctaattt
//
LOCUS       DAOQNZ010000161         4027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10180_length_4027_cov_3.668177, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000161 DAOQNZ010000000
VERSION     DAOQNZ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10180_length_4027_cov_3.668177"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="PKW73_04110"
     CDS             complement(<1..1657)
                     /locus_tag="PKW73_04110"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNA72494.1"
                     /translation="MAVTTGEDLLARIKPLRDDVRELGFVLGDTIKRFEGETVFAYVE
                     KLRALFKQIHKDKAAGLDTASLQAELVQLIEAMPLSESACVIKAFLTYFDIINIAEQN
                     HRLRRRALRDQKQAQESLPSLEPGSLSEVFAELGQEDYRSELEQLLKSLDIEVVFTAH
                     PTEITRRTVLLKQLALASLLHKKDHPPLSQAEQNKLKSGLKSVVESLWLTEHVMHFKP
                     AVMDEVRYGIYHFDNVVFDAVIDVHERLLADLPASAADKSVNFITFGSWIGGDRDGNP
                     FVTCDVTRKTLAYQKQVIFGRYLKELETLFNELSHSYRWLASEGLELKPALVKLKQSL
                     ESEAEKFPQVFERHGERYKLEPFRLKLLYIQARLRATLAGQDGEGKNEGKSEGRSEGQ
                     QDYCTATELRRDLEMIEAVLVEAGCVESGRSLRRLIYGVDIFGFHLAKLDIRQHSKRH
                     RQALSEVLAGLQICPDFESLPEEEKVAFLSKELTGKRPLLPRLLSFSPATSETIEVFR
                     TMYDLRKLYGDEALDTYIVSMTERPSDLLTILLFARESELMAPP"
     gene            complement(1673..3151)
                     /locus_tag="PKW73_04115"
     CDS             complement(1673..3151)
                     /locus_tag="PKW73_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72495.1"
                     /translation="MKAVSGRWHRQVLLLSIAFTLSGASFLDAGLAQARDLDYFVPVN
                     FRNGQVEKFVAPTYSQSDLKKLKRFLTEKGTLDLRRYSSGGHSAVTIGTDRSLESAIS
                     GLLIMHWDRDNIMQALAEREDGLSQWHSGLKASLKHHYRYRHRFIDMIEGKSDKKSQM
                     QRPHIRYNAVSLDELSEPWGHAQNDALSFITFLLFFDALESNRSIEGQEALAYAVLLP
                     HYLDAVKVEHDLEMGAWEDKRAVHASSLLVVLAALRQEYEYVTRHGTITAKIDGREYR
                     VDEAFLRQLLLRVKKSLKTILPNEYVESDQNDTRADKSKVRKFDSALVNGLLLGALSA
                     NGIISDKMTATVLAGLEANLMGADGVARYPHDIWDGRTDRHDLKRGQEAQWCHVSPLM
                     SVVYGDLYRRTGHKVYFDKQTEHFNRALSHINERYAIPEAYIVEPPGAGKAGSNAKEK
                     TKVNWIADANEPLAWGQAALRLAFNGMEKSLVHSADLKKTHK"
     gene            complement(3168..>4027)
                     /locus_tag="PKW73_04120"
     CDS             complement(3168..>4027)
                     /locus_tag="PKW73_04120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNA72496.1"
                     /translation="KDTGYLPQYKELLMSCFMQWQAALKDKVPELSFVFVDASKKDQA
                     AILCTWTKKRETMTSDKEDGRANLVIDEEGIKSVTLTLLTLAPPGTKELSPNYLSRVA
                     LHEIGHALGLSGHSPDAADIMFGSITVSDRPVALSSRDIATLSLLYSGLDKDKAPVVV
                     NPETMAQTAGGRALMLSNESNLAMKAGRFDEALKKMEEAYKLDPTNALIARNLGGIYA
                     NFASISMMARNPVLSESYFKKAITTLEKDAANKPVLSQVQRAYGSLLMMTGRASEAEA
                     MLKKVVPNK"
BASE COUNT          901 a         1062 c         1063 g         1001 t
ORIGIN      
        1 tgggaggcgc cataagctcg ctctcccggg caaagagcaa aatggtgaga aggtcggaag
       61 gccgctccgt catgctgacg atataggtat cgagggcttc gtcaccatag agtttgcgca
      121 gatcgtacat ggtgcggaag acttcgatgg tttcactggt ggcgggagag aaggacagaa
      181 gccggggcag aagtgggcgc ttgccggtaa gctctttact caggaaggct actttctctt
      241 cttccggtaa actctcaaaa tcagggcaga tttgaagacc tgccagtacc tcagaaaggg
      301 cctggcggtg gcgcttgctg tgctgcctga tatcgagttt ggccagatgg aagccgaaaa
      361 tatccacgcc atagatgagg cgccgcagac tcctgccgct ttcaacacag cctgcttcca
      421 caagcacggc ctcgatcatt tccagatcac ggcgaagttc tgtggcggtg cagtaatctt
      481 gctgcccttc acttctccct tcacttttcc cttcattttt cccttcaccg tcttggccgg
      541 ccagggtagc ccgcagccgg gcttgaatat agagcagctt gaggcggaag ggctcaagct
      601 tgtagcgctc accatgtctt tcaaagacct ggggaaattt ctctgcttcc gactccaggg
      661 actgcttcag cttaaccagg gccggcttga gctcaagtcc ttcggaggca agccatctat
      721 atgaatgact gagttcattg aagagggttt ccagctcttt caaatagcgt ccgaaaatca
      781 cttgtttttg ataagcaaga gttttgcggg tgacatcgca ggtgacaaag ggattgccgt
      841 cccggtcgcc gccgatccag gagccgaagg taatgaaatt cacgctcttg tcggcagcgc
      901 tagcgggcaa atcagccagc agacgctcgt gtacatcgat gacggcatcg aagacaacgt
      961 tatcgaaatg gtaaattccg taccgtacct catccattac cgccggcttg aaatgcatga
     1021 cgtgctcggt cagccagaga gattctacaa ccgacttcag tccgctcttc agtttgttct
     1081 gctcggcctg cgatagcggc ggatgatctt tcttgtgcag caaagaggct agagccagtt
     1141 gcttgagcaa aaccgtgcgc ctggtaatct cggtggggtg ggcggtaaag acgacttcaa
     1201 tatctaggct cttgagaagt tgctcaagtt cacttctata atcttcctgc ccgagttctg
     1261 caaagacctc actgagactg cccggctcaa gggagggcag cgactcttgt gcctgcttct
     1321 ggtcgcgcaa agcacgcctg cgcaggcggt gattttgctc ggcaatattg atgatgtcaa
     1381 aataggtaag gaaggcctta atgacacagg cactctctga tagaggcata gcctcaataa
     1441 gctgcacaag ctcggcttgc agggaagcag tatcgagacc ggcagccttg tctttgtgta
     1501 tctgcttgaa gagggcccgc agtttctcta cataagcaaa tacggtctcg ccctcaaagc
     1561 gcttgatggt atcgccgaga acaaagccca actcccgcac atcgtcccgc aagggcttga
     1621 tgcgtgccag cagatcctca cctgttgtta ctgccactga agctctcctt acttacttat
     1681 gagtcttttt caaatcggcc gagtgtacca gacttttttc catgccgtta aaggctaatc
     1741 ttaaggcagc ctgcccccag gctaacggtt cattggcatc agcgatccag ttgaccttgg
     1801 tcttctcctt cgcattacta cctgccttac cggcacctgg cggctctacg atataagctt
     1861 cgggaatagc gtagcgctca ttgatatggc tcaaggcgcg gttaaaatgt tccgtttgct
     1921 tatcgaagta gaccttgtgt ccggtgcgcc tgtacagatc accgtagaca accgacatca
     1981 gcggagagac atggcaccac tgggcttcct gtccccgctt cagatcatgc ctgtcggtgc
     2041 ggccgtccca gatgtcgtgg ggataacgag cgacgccgtc ggcccccatc aaattggctt
     2101 ccagaccggc taagacagtg gcggtcattt tatcgctgat gatgccgttt gcagaaaggg
     2161 cccccagcaa taagccgttc accagagcgc tatcgaattt gcgcaccttg ctcttatcgg
     2221 ctctggtgtc gttttgatcg ctttccacgt attcattcgg aagtatggtc ttgagactct
     2281 tcttgacccg caagagcaac tgtcgcaaga aagcttcatc gactctgtac tccctgccgt
     2341 caatcttggc ggtaatggta ccgtgcctgg tgacatactc gtattcttgc ctgagggctg
     2401 ccagtaccac aagtaagctg gaggcatgca cggcacgctt gtcttcccag gcacccatct
     2461 ccagatcatg ctccactttg acagcatcga gatagtgggg caagagcacc gcataggcaa
     2521 gggcctcctg cccctctatg gaacggttgc tctcaagggc atcaaagaaa agcaggaaag
     2581 taatgaagga aagggcatcg ttttgagcgt gtccccaggg ctcgctcagc tcatcaaggc
     2641 ttacagcgtt atagcgaata tgggggcgct gcatttgact tttcttgtcg cttttgccct
     2701 cgatcatatc tatgaagcga tgacggtagc ggtagtgatg cttgagggaa gccttgagcc
     2761 cgctatgcca ctggctgaga ccatcttctc tttcggccag agcctgcata atattgtcgc
     2821 ggtcccagtg catgatcaaa agcccgctta tggccgattc cagagaccga tccgtgccga
     2881 tggttacggc tgaatgtccg cctgagctat agcggcgcag atctagcgtg cctttttccg
     2941 ttagaaaacg cttgagtttc tttaaatcac tttgactgta agtgggcgca acaaattttt
     3001 ctacctgacc gttgcgaaag ttgaccggca cgaagtagtc gaggtcgcgg gcctgagcaa
     3061 gaccggcatc taagaaggaa gcgccgctga gggtaaaagc tatggaaagc aagagaactt
     3121 gacggtgcca ccgaccagag actgctttca ttttccaata ccctcttcta tttatttgga
     3181 actacttttt ttagcatggc ttccgcttcg cttgcccgcc cggtcatcat aagcaggctg
     3241 ccataggctc tctgcacctg cgaaagaacc ggcttattgg cggcatcctt ctcaagggta
     3301 gtgatggctt ttttgaagta tgactccgat agtaccggat tgcgagccat catggaaatg
     3361 gaggcgaaat tggcgtagat accgcccaga ttacgagcaa tcagggcatt ggtggggtcg
     3421 agcttgtagg cttcttccat tttcttaagg gcttcatcaa accgccctgc tttcatagcc
     3481 aggtttgact cattgctcag cattaaagca cggccgcctg ctgtttgagc catggtttcg
     3541 ggattcacca ccaccggtgc cttgtcttta tcaagcccgc tgtatagaag agagagggtg
     3601 gcaatgtcgc ggctgctcaa agccacgggt cggtcgctta ccgtgatgga accgaacata
     3661 atatcggcgg catcgggact gtgcccggat agaccaaggg catgaccgat ttcatgcagg
     3721 gccaccctgg agagatagtt aggggataac tcctttgttc cgggtggagc cagagttaga
     3781 agcgtcagtg tgaccgactt gatgccttcc tcgtctataa ccagattggc ccggccgtct
     3841 tccttgtcgg aagtcatggt ttcacgtttt ttcgtccagg tgcaaagaat ggccgcttga
     3901 tcctttttgc ttgcatccac aaagacaaag ctaagctcgg gcactttatc tttcaaagcc
     3961 gcctgccact gcataaagca agacataagc agttctttgt actgaggtag atagcccgta
     4021 tctttct
//
LOCUS       DAOQNZ010000162         4027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10181_length_4027_cov_3.319235, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000162 DAOQNZ010000000
VERSION     DAOQNZ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10181_length_4027_cov_3.319235"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1125)
                     /gene="cpaB"
                     /locus_tag="PKW73_04125"
     CDS             complement(163..1125)
                     /gene="cpaB"
                     /locus_tag="PKW73_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HNA72497.1"
                     /translation="MNPLRTLFLMLSRTPPALMLLAIIGLAVLITFTVTTTNDGEKAA
                     LEARLKEAQEKAGAKTKVVFVTRDIMEGAVIEADALTEKDMEVGRVPMDALPVASNAI
                     GRRAKFPVAAGQILSSHDLAPIGLSLDFNSRLPEGMRAVTFAVDANSGVAGFVSPQSH
                     VDVLSMVGSGQETKVAPILSDVEVVAVGQQFEREAKGQVTPASSVTVAVSPEDTNKLI
                     KAVAASKLYLSLRNDKDHMPVTTVDVTALFAAPKRMAQDPLGVPVTALEPPPLPNPLQ
                     MENLPLPSGAVNTSASASVPMPPPLHEIEVWSGSRKDVIQVTQK"
     gene            1612..2286
                     /locus_tag="PKW73_04130"
     CDS             1612..2286
                     /locus_tag="PKW73_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72498.1"
                     /translation="MTITLDSKAEDIASTLELDDSELEAELALELKSLTSTLEMTRLD
                     VVEKPLPEVPEAMDFHPPQADAPAASPASGADEGWRIARQAMVRDFLVLSGKGFAGFV
                     VLAVVGFSVLWGFDKVRQINPQTVKSSLAKIFPVLNGLNNSPSNSEPVVSIPQETPAP
                     TEKVRTASRSRSRNSLKERRNARRNQSRVKVARRYSPYSYWAADHPNGGSITYSDGTI
                     TEYTWH"
     gene            2318..3280
                     /locus_tag="PKW73_04135"
     CDS             2318..3280
                     /locus_tag="PKW73_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72499.1"
                     /translation="MLPPKVVPGEKSVVEYQRLLIRYQSLTWLAQMSQVSKIILQMSP
                     EDAMSESDRRKMLALMTAVQASVFVTDTITGVQDTAADVVKFAASEGGKLLDKNELTK
                     KAKDKAASAFGFVSQLANDAVHKVLEDVVLPHVGLPVHYVPEGGTGEQYLTMALRFEK
                     IGYVEPIRAALNKATSAGSGDRARVLLATRVCRTVVRPESMSKFVDALKFIYTKKESQ
                     GKELLLELLRDEPGFDWIYPRLAFMELVSGNEERARDLLARVLRQNPDFLPGWSMLAT
                     VELAAWQIEKMEKHLERMVKLDPLAEEVAKTNQMLSLINQGGLR"
     gene            3406..>4027
                     /locus_tag="PKW73_04140"
     CDS             3406..>4027
                     /locus_tag="PKW73_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72500.1"
                     /translation="MRQKKFDDAYAAVTKSLKLYPREPWLLAQAAQICIYLEKDEEAS
                     LYASKAFKAKPWDAETTYWLANVFRQLDAYDKSIQVLETGLRDFPANRGLRFLLGENY
                     LRRGEPAKSEAIFQALCRESPGSFMNWLNLMDAQEKQEKWQSLVESGKLMEKAASAVP
                     VTAGKPAVKDSVIFARCLSHQAKGYMKLGRWSEAKTCLERVLRIMPL"
BASE COUNT          928 a         1002 c         1123 g          974 t
ORIGIN      
        1 cccataaagt cttgccgcca tccggtggtg cggccattga gatgatagaa attcaagagc
       61 cgtcctccaa aaacaagaag cagcaaaaag gcaaaaatac aaattgagag aaagccggtt
      121 ttgcagctct ctctcaactt gtggtggctt tggcctgggg aattattttt gagttacctg
      181 aatcacgtct ttgcggctgc ctgaccacac ttcaatctca tgaagaggcg ggggcatggg
      241 cacggaggcg gaggcggaag tgttcactgc accggaggga agcgggaggt tctccatctg
      301 caaggggttt ggcagcggag gcggctccag ggcagtcacc ggcactccga gcggatcctg
      361 agccattctc ttaggagcgg cgaagagggc cgtgacatca actgtggtga ccggcatgtg
      421 gtctttatcg ttgcgcaatg atagatagag tttgctggct gccaccgctt tgattagctt
      481 attagtgtct tccggtgaca cggctacggt gacggaactg gccggagtga cctgaccttt
      541 ggcttctctt tcaaactgct ggcccacggc taccacttcc acatctgaaa gtatcggcgc
      601 tactttcgtc tcctgaccgc tgcccaccat ggagagcaca tccacgtgac tctggggcga
      661 gacgaagccg gccacaccgg aattagcatc gacagcaaag gttactgctc tcatgccttc
      721 gggcagacgt gagttgaaat caaggctgag accgataggg gccaggtcgt gtgaagagag
      781 aatttgacct gcggccacag gaaacttggc tcttcgacct atggcattgc tggccacggg
      841 cagggcatcc atgggcaccc ttcccacttc catatccttt tccgttaagg catcggcttc
      901 aatgacggca ccttccataa tgtctctggt gacaaagaca accttggttt tggcaccggc
      961 cttttcctgg gcttccttga gtctggcttc cagagctgct ttttcaccat catttgtagt
     1021 tgtgactgtg aaagtgatga ggaccgccag tccgattatg gcaagcagca tcagtgctgg
     1081 aggtgtgcga gagagcatga gaaatagggt tcgtaatgga ttcattactg cctcctggtt
     1141 atgtagaatt gacgaagctg ggcgttgccg tgtactgcac cattaccggt gcagtactca
     1201 atattctttt gcgcaacgcc atttctagtg ccgccgacct tggctcctgg ccaggaccga
     1261 acagcaactt ttcccattct ttcactgcct cagccgtcga taataggaaa aaccctgaac
     1321 cttgtcagga gataaggaca gtaaccggaa accggcgcta ttgccccgtg tttatcggca
     1381 gctcggctgt aaatcggttt acaagcgggt ggatttcttc gtaaaacttg atgagttacg
     1441 atttatccct gacaagcttc ctgtctgaaa gtgaaaaggc gcacctctct ggccggggtg
     1501 aaaggtgctg cagccagtat tagccgggct aaatgttgac atcggaagac atctatgaca
     1561 ccttagcagc cgaactaatt ttcttctttc gagtctatat ataaggttgc aatgacaatt
     1621 accctcgata gcaaagcgga agacatagct tccacgctgg agctggatga tagcgagctt
     1681 gaggctgaac tggctctgga gctgaagtct cttacatcaa cgctggaaat gaccaggttg
     1741 gatgttgtgg aaaagcctct gccggaagtt ccggaagcta tggatttcca cccgcctcaa
     1801 gctgatgccc ctgctgcctc ccctgcctcc ggcgctgatg aaggttggcg cattgcccgc
     1861 caggctatgg tgcgtgattt cctggtgctt tcgggcaagg gctttgccgg ttttgtggtg
     1921 cttgccgtgg ttggtttttc cgtgctctgg ggtttcgata aggtcaggca gataaatcca
     1981 cagaccgtga aaagctcctt agcgaaaatt tttccggtgc tcaatggctt gaacaatagt
     2041 cccagcaatt cggagcccgt ggtttccatt ccgcaggaga cgccggcacc aaccgagaag
     2101 gttcgcacag ccagccgcag tcgcagccgc aacagtctta aggaacgccg caatgcccgc
     2161 cggaaccaat ctcgggtcaa ggtggccagg cgctattccc cttattctta ttgggcggcc
     2221 gaccacccca atggtggcag cattacttac tctgacggta caataacaga atatacctgg
     2281 cattaagtta gttcgcccag cgaggttcta agcggcaatg ttgccaccga aggtagtgcc
     2341 cggcgaaaag tcggtggtgg agtaccagcg cctactgatt cgctatcagt ctctcacctg
     2401 gttggcgcag atgtcccagg tgtccaagat aatcttgcag atgtcgccgg aagacgccat
     2461 gagcgagtct gatcgtcgca aaatgctggc tcttatgacg gccgtgcagg ccagtgtttt
     2521 tgtaaccgac accatcaccg gggtgcaaga caccgccgcc gacgtggtga aattcgccgc
     2581 cagcgaaggc ggcaaactac ttgataaaaa cgaattgacg aaaaaggcta aagataaagc
     2641 cgccagcgcc tttggttttg tcagtcagct cgctaacgac gccgtgcaca aagtgctgga
     2701 agacgtagtt ttgccccatg tgggactgcc cgtacactat gtgccggaag gtggtacagg
     2761 cgaacagtat ttgaccatgg ccctgcgctt tgagaaaatc ggctatgttg agcccatacg
     2821 agcagcgctc aataaggcga cgtcagccgg cagcggcgat cgcgctcgag ttttgcttgc
     2881 caccagggtt tgccgtaccg tggtgcgacc tgagagtatg agcaagtttg tggatgctct
     2941 caaatttatc tatacaaaga aagagagtca gggcaaagag ttattgttgg agcttttgcg
     3001 cgacgagccg ggctttgact ggatctatcc ccgactggct tttatggaac tggtctcagg
     3061 caatgaagaa agagccagag atttacttgc ccgcgtgctc cgccaaaacc cggactttct
     3121 gcccggctgg tctatgctcg ccaccgtgga actagctgcc tggcaaattg agaagatgga
     3181 aaagcatctg gagcgcatgg tcaagcttga tcccctggct gaggaagtgg cgaaaaccaa
     3241 tcagatgctc agtttgatca atcaaggcgg tctgcgttga taactgggag aatgaccggc
     3301 aagcgttatt ctatcggtct tttttgcttg accatgctgt gcttttcgtc tgtctctctt
     3361 gcggctgaaa gtctggctgg ctgtcgaaag caggtagaag agtctttgag acagaaaaag
     3421 tttgatgatg cctatgctgc cgtaacgaag tctctcaaac tctacccccg tgaaccatgg
     3481 ttactggctc aggctgctca gatttgcatc tatctggaga aagacgaaga agccagtctc
     3541 tatgcgtcca aggcatttaa ggcaaagccc tgggatgctg aaactaccta ctggctggct
     3601 aacgttttca ggcaattgga tgcctatgat aagtccatcc aggtgcttga gaccggtttg
     3661 agggatttcc ccgccaatcg aggtctgcgc tttttgttgg gcgagaacta cctgagaaga
     3721 ggtgaaccgg ctaagagtga ggctattttt caggctcttt gtcgggaaag tccgggcagt
     3781 ttcatgaact ggttgaatct gatggatgcg caggagaagc aggaaaaatg gcagtctctg
     3841 gtggagagcg gcaagctcat ggaaaaggct gcctctgccg tacctgtaac tgctggtaag
     3901 cctgccgtga aagactctgt tatttttgca cgctgcttga gtcaccaggc caaaggctat
     3961 atgaaacttg gtcgctggtc ggaggctaag acctgtcttg aaagggtttt gcgtatcatg
     4021 cctctgg
//
LOCUS       DAOQNZ010000163         4026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10186_length_4026_cov_3.349282, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000163 DAOQNZ010000000
VERSION     DAOQNZ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10186_length_4026_cov_3.349282"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PKW73_04145"
     CDS             complement(<1..782)
                     /locus_tag="PKW73_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72501.1"
                     /translation="MSDATSTPPGNPANPILPVRAIAVNTFREVARDKVVYTFFLFAV
                     IISFFGLALGSLSVGQDVRIILDLGMCGISTIGGIIAVFAGANLVYKELEKKTCYLIF
                     TKPVAAWQFILGKYIGLSICLLFLILGMGAFLALTVYLADPAHLLIMTERLKWIGLSL
                     LLTYLELLLIIAAAEFFSTFSSPIMSVVFTVSFWLIGHSSASLQELSRIFTNPAMKQL
                     SAILYWTLPDLQSLTRARAVIMYGKLPASELTLYLSAYLIAYV"
     gene            complement(779..1561)
                     /locus_tag="PKW73_04150"
     CDS             complement(779..1561)
                     /locus_tag="PKW73_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA72502.1"
                     /translation="MSEATAVESSKAVIEVRNLTKDRESFLRKPLRVLDGLSLKVEKG
                     MTYGLVGPNGAGKTTTIKLLLGLLRADQGSIAVLGAPPGDKAALCKIGFLPESPYFYS
                     HLTGREFLTFSGQLFGLSGKALNERIEQLLATVSLQKKADERTGRYSKGMLQRLGLAQ
                     ALINDPELLFLDEPMSGLDPIGRMDMRRIIEDLKNGGKTVFFNSHLLPDVAALCERVG
                     LLSGGKLVAEAAIEEISPQRDARALEEFFLKHIQAPAEEVVT"
     gene            complement(1561..2391)
                     /locus_tag="PKW73_04155"
     CDS             complement(1561..2391)
                     /locus_tag="PKW73_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA72503.1"
                     /translation="MQAKSEAPKGAATPKAEIKGPDQIHNWLSLYAESRDPALRDSII
                     QAALPLVKRIAYGLARRSTDPVEDLIQVGSIGLIKAVDQFKPDAGAKFQTYATHLITG
                     EIRHYLRDKTAMIRAPRELQELSFRINRLVQNLTAKLGREPSDIEIASELQIPVSRVN
                     EAYEVDRRRTLISLDQALSNDAGSEQSLIDTLVDSRYQSNQLAKEDRFMLAEAIKQLR
                     DGLREVVQLTFYEDLSQTEVARRLGISQMQVSRRLRAATTELNKIITQSKKANGPKGG
                     "
     gene            2870..3883
                     /locus_tag="PKW73_04160"
     CDS             2870..3883
                     /locus_tag="PKW73_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72504.1"
                     /translation="MAETNPSDKGDRQNLEQIENAAPSTSDILQARNVSSEQVEAAWA
                     ANPDRSDGSATPRNRSLLAKQGESLRDFVDKQNASIELTERDEFGQEVVIAARVMQAK
                     PSTNKQLIAYDLQDVQKQLNAGTKKIGTFLECSKQSLTSFEAGNPIYPHLGKEDNFVS
                     YSACRLANSEYPELSKRIGVGKGHIDKHLIAATNRLEVAFYKQGVDTGQDKYVQEHGQ
                     DDCIGQTVSIGPAQMQIRHIERLVKEFPVQLGKFALDPLRAALKVENAPHFVGGYFAE
                     VIQHLDRGTKPDYIGGLDWAGVKKFWSEGDLNAALIYAYNPKEDHVASVKNQLKTIQL
                     KGF"
BASE COUNT         1004 a          950 c         1106 g          966 t
ORIGIN      
        1 acataggcta tgagataagc cgataggtaa agggtaagtt cactggccgg aagcttgccg
       61 tacatgatca cggcccgtgc tcgggtcaga ctttgcaggt cgggcaaggt ccagtagaga
      121 atggcgctca gttgcttcat tgccgggttt gtgaagatac ggctgagctc ttgcagggag
      181 gcggacgagt gtccgataag ccagaatgaa actgtaaaga caacagacat aatcggtgat
      241 gaaaatgtcg aaaagaattc tgccgcagca attatcagaa gcagttccag gtaagtaagc
      301 agaagtgaaa gccctatcca cttgaggcgt tccgtcatga tcagaagatg ggccgggtcg
      361 gccagataga cggtgagcgc gagaaaggct cccatcccca gtattagaaa gagcaggcaa
      421 atcgaaagac cgatatattt gcccaggata aactgccagg ctgccaccgg ttttgtgaaa
      481 atcagatagc aggttttctt ttccagttct ttataaacaa gattggctcc ggcaaagacg
      541 gcaatgatac cgccgatagt gctaatgccg cacatgccca gatcgagaat gatacgcaca
      601 tcttgcccca ccgataatga gccaagcgcc agaccgaaga aggaaataat cacggcaaat
      661 aggaaaaagg tgtagaccac cttgtctcgt gccacctctc gaaaggtgtt tacggcaatg
      721 gcccgaaccg gcaaaattgg gttggcgggg ttgcccggcg gcgtagaggt agcgtcgctc
      781 atgtgaccac ctcttcggcc ggcgcttgga tatgtttgag gaagaattct tccagagccc
      841 gagcatctcg ctgggggctt atctcttcta tggctgcttc cgccaccagt ttcccgccgg
      901 aaagcaagcc tactcgttca cagagcgcgg ccacatccgg tagcaggtgg gaattgaaga
      961 agactgtttt gccgccgttc ttgaggtctt cgataatgcg cctcatatcc atgcgcccga
     1021 ttggatcaag tcccgacatc ggttcatcca gaaagagcag ctcggggtcg ttgatgaggg
     1081 cctgggccag ccccagcctt tgcagcatac ctttggagta gcgtccggta cgctcatcgg
     1141 ctttcttctg caaggaaacc gtagccagca gttgctcgat ccgctcattc agcgcttttc
     1201 cactcagtcc aaagagctga ccggagaaag tgaggaattc tcgaccggtc aaatggctgt
     1261 aaaaatatgg agattccggc agaaagccaa tcttgcacag ggcggctttg tctccgggcg
     1321 gcgcccccag tacggcaatg ctaccctggt cggcccgcaa aagacccagc aggagcttga
     1381 tggttgtagt tttgccggcg ccgttgggac cgacaagacc ataggtcatg cccttttcta
     1441 ctttcaggct gagtccgtcc aggacgcgca agggcttcct caaaaagctc tctcgatctt
     1501 ttgtgaggtt tcttacttct atgactgctt ttgatgattc gaccgcagtc gcttcactca
     1561 ttagccgccc ttcggaccgt tggctttctt actctgggtg atgattttat tcagttcggt
     1621 tgtagcggct ctcagtctgc gggagacttg catctgggaa attcccaggc gacgcgctac
     1681 ttcggtctga ctcaaatctt cgtagaaggt tagttgtaca acttctctca aaccatctct
     1741 caactgcttg atagcttccg ccagcataaa gcggtcttct ttagccagtt ggttactctg
     1801 gtagcgagaa tcaaccaggg tgtcgatcaa ggattgctca gagccggcgt cgttggagag
     1861 ggcctggtcg agggatatga gggtgcggcg tctgtctact tcgtaggctt cgttcacacg
     1921 gctgacaggt atttgcaact cgctggcgat ctctatatct gaaggctcgc gaccgagttt
     1981 ggcagtgaga ttctgtacca gtctgttgat acggaaggag agttcttgca actccctggg
     2041 ggcgcgaatc atggctgtct tatcgcgcag gtaatggcgg atttcgcccg taatcaggtg
     2101 agtggcataa gtctggaact tggcaccggc atcaggtttg aattgatcga cggctttgat
     2161 aagaccaata gagccgacct ggatgagatc ctcgacaggg tcggtggaac gacgagccag
     2221 accgtatgct atgcgcttca ccaggggcaa agccgcctga atgatggaat cgcgcaatgc
     2281 cgggtctctt gactcagcat agagagataa ccagttatga atctggtcgg gcccctttat
     2341 ttctgcctta ggggtggcag cacctttagg tgcctcactt ttagcttgca aatccttgct
     2401 caccgcgtta cttgtgctta ctgggttaag ggagttcgtt gtagtcgttt ccattactat
     2461 tatctataga tccaatcgca gattaccaga ctcattatat aaatgctacc gtgatttccg
     2521 ccgcgggctg gctgtcaagg cagttccgat aattgccaag tgcgtttctc caaagcctct
     2581 ccttaagcca agttcaggaa ggcaatgctt tctgccaagg ggcttttcgg ctctcggaag
     2641 ttgctatgaa cctggcttgt ggtgcggatt tgttcccggg ctgccgataa tttttcgggg
     2701 tatggtgatt aattctttct aagggcgtcc gttttgccgg ttaatggcag gcgaaaagtg
     2761 gcagcggact gattttggac ggctgcaagg gaaacgtcct tactggaagt gcttgttttc
     2821 tgaacatatg atggaacagt gggcttgaga caccgagagg tgctggttga tggcagagac
     2881 aaatccgagc gacaagggag atcgacaaaa tcttgagcag attgaaaacg cggcgccgag
     2941 tacctcggac attttgcaag cgaggaacgt ctcaagcgag caagtggaag cagcctgggc
     3001 tgcaaacccg gatcgaagcg acggctctgc cacaccgaga aatagatcct tattggcaaa
     3061 acaaggagaa tccttgcgtg actttgttga taaacagaac gccagcatcg agctaaccga
     3121 gcgagacgaa tttgggcagg aagtagttat agcagccaga gttatgcagg cgaaaccaag
     3181 tacaaataag caattaattg cctatgacct ccaagatgtt caaaaacagt taaatgctgg
     3241 tactaagaaa attggcacct ttttggagtg ctcaaagcag agcttgacca gtttcgaggc
     3301 tggaaatcca atctatccgc atttagggaa agaggacaac tttgtttcct actcggcatg
     3361 tcgtttggcc aattccgaat atcctgaatt gtcgaaacgt attggggtgg gtaaagggca
     3421 tattgataag caccttattg ccgccacgaa ccgcctagaa gtggcattct acaagcaggg
     3481 agttgatact gggcaagata agtatgtaca agagcacggg caggatgact gtattggaca
     3541 gaccgtatct atcggaccgg cccagatgca gatccggcat atagagaggc ttgtgaagga
     3601 gtttccagtg cagttgggga agttcgcgct agaccctctg agagcggcat tgaaggtcga
     3661 gaacgcgcca cactttgtcg gaggctattt cgcagaagta attcagcacc tcgacagggg
     3721 cacaaagcct gattacatag gtgggctaga ttgggctggt gtaaagaaat tttggagtga
     3781 aggcgatttg aacgccgcct taatctatgc ttacaatcct aaggaagatc acgttgccag
     3841 cgtaaagaat caattgaaaa ctattcagct gaaggggttt tgacgcgttt gttgtctagc
     3901 cacttgacta agaaaaagcc cagtgcgaat ccttcaattg gaccggcgaa gagccaaagc
     3961 aaaagagctt ctacactctc aataggattg ccaacgtagg catctctaaa ggtgccatac
     4021 caccag
//
LOCUS       DAOQNZ010000164         4022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10201_length_4022_cov_4.289640, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000164 DAOQNZ010000000
VERSION     DAOQNZ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10201_length_4022_cov_4.289640"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..904)
                     /locus_tag="PKW73_04165"
     CDS             complement(134..904)
                     /locus_tag="PKW73_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HNA72505.1"
                     /translation="MTASRSVSQKKARSRKGAAKTLPVLVESNCQSGCSDCALAKGGK
                     CGGSCPGASSTAASLMPEFKVIPGISSRHQHGVDYDQGDLLILTKMLGSALGPGPMCF
                     DAHHGGAISQAVQEKHFQGAAGEHLAVEFPPQEGSPSGRKRHAVVVGLGRPDEVSKRR
                     ICGFFNYALDLCAHYQAETLVLPIFPYRLSQGSLSLTATAAILRCITAERVRRGTLSG
                     VKEIRLLCAPQAKSHLSRGLAVQHTLCSQCHIPGFAEG"
     gene            complement(976..1773)
                     /locus_tag="PKW73_04170"
     CDS             complement(976..1773)
                     /locus_tag="PKW73_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72506.1"
                     /translation="MLCPHCSSPRHILCDEGFRVNVNFSTFGSADDAMKSIKAIAASC
                     GETHLLGPQPDSQKAPVVTGSMAKVKRDESVLLVLPSFQDTGLGVYGRGVLTALSPAN
                     LSEVKDGVVKPKFDDQVFDGAFASTHALTLAPIDGQRLSVLVIGLGKRDDCSKKGLCG
                     LIGTAVDSAVSGLYSHLVVAISDFTGAPIYGQQLGAVSRCRLSIAVVEDAADLVLSEM
                     TLVVNVDQVAGLCQGIDIAGPLCSHCNHPRAGTIKLGRPEPHNQGYN"
     gene            complement(2429..3184)
                     /locus_tag="PKW73_04175"
     CDS             complement(2429..3184)
                     /locus_tag="PKW73_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72507.1"
                     /translation="MNCANQDLTVSRVLTIRERPWCNKRCQHALGRFYLDGSTGIYYQ
                     EGTSPEHIERLRSVFPFVSTPLLQAGLKLGLTMTTCEGLTPNGDSSTIYADFRSYTKD
                     GISPHIVMGSESLEEDWILPHLVHELSHIFYALLPESLRQRWIELLPPYSSLNSWHEV
                     TGYAQRNREAWLRRLKETAGWSERQNCCRVMLERYATETFCETVAVLSSPTYLSRRCN
                     IPLAGRRAFLEELGLFFMGASGSDDSMHLHQAA"
BASE COUNT          982 a         1096 c         1118 g          826 t
ORIGIN      
        1 gtctttctcc tgtgcgaatt agaaagtttg agacggcggg cagtgcagcc tgagcggatg
       61 caaaactctg caccgccgtc gcgttttgtc gatttgcaag aaactcgccg aaaggcagag
      121 tcgacgggcg actctagccc tcggcaaaac cgggaatgtg gcactgactg cagagcgtat
      181 gctgcacggc caggccgcga ctgaggtgcg acttggcttg cggagcacag agaagacgaa
      241 tctccttcac cccggagaga gtgccgcgcc gcacccgttc agcggtgatg cagcgaagga
      301 tggcggccgt agccgtcagg ctcagggacc cctgggagag ccgatacggg aatatcggca
      361 agaccagagt ctcagcctgg tagtgagcgc agagatcgag ggcgtagttg aagaagccgc
      421 aaatgcgccg cttcgacacc tcatcggggc gccccagacc aaccaccact gcatggcgtt
      481 tgcgcccgga cgggctgcct tcctggggcg gaaactccac ggcaaggtgc tcgccggctg
      541 ccccctggaa gtgcttttcc tgcacagcct ggctgatggc accgccatgg tgagcatcga
      601 agcacatggg gcccggtccg agagcggagc cgagcatctt ggtgagaatg agcaagtcgc
      661 cctggtcata atccacgcca tgctgatggc gcgaactgat gccggggatc accttgaact
      721 cgggcatgag ggaggcggca gtgctggaag cgccggggca ggagccgccg cacttgccgc
      781 ctttggccag agcgcaatcg ctgcagccgg actggcaatt gctttccacc agcaccggca
      841 gggtcttagc agcgcccttg cgagaacgcg ctttcttctg ggaaacagat cttgaggcag
      901 tcatactgtc ctccttttct atggttgacg tttcaagaaa ttgaggttgc ctgaaagccg
      961 cttgcgaagc ggttctcagt tatacccctg gttgtgcggc tcgggacgac cgagcttgat
     1021 ggtgccggcg cgcggatggt tgcagtggga gcagagcggt ccggcgatgt cgatgccctg
     1081 gcaaagacca gccacctgat ccacattgac caccagggtc atttcgctca ggaccagatc
     1141 agccgcatct tccaccacgg caatcgagag ccggcagcga gagacggcgc ccagttgctg
     1201 accgtagatg ggggcaccgg tgaagtcgga aatggccacc accaggtgcg agtagagccc
     1261 gctgacggcg ctgtccacgg cggtgccgat caggccgcag agacccttct tgctgcagtc
     1321 gtcgcgcttg ccaagcccga tcaccagcac gctcaggcgc tgaccgtcga tgggggccag
     1381 agtgagcgca tgggtggaag cgaaggcgcc gtcgaagacc tggtcgtcga acttgggctt
     1441 caccacaccg tctttgacct cgctcagatt ggcgggggaa agagcggtga gcacgccgcg
     1501 cccatagaca cccagaccgg tgtcctggaa gctcggcaga accagaagca cgctttcgtc
     1561 gcgcttcacc ttggccatgg aaccggtcac gaccggcgcc ttctggctgt cgggttgggg
     1621 gccaagcaag tgggtctcgc cgcaagaagc agcaatggcc ttgatgctct tcatggcatc
     1681 atcagccgaa ccaaaggtag agaaattgac gttgacacgg aatccctcgt cgcaaaggat
     1741 gtgacgggga gaactgcaat gcgggcaaag catagtttta actccagttt agttgtggtt
     1801 tcgccttcaa ggaaaatccc tgagggcact tcgccatcac ttgcacacca ccagtcaaat
     1861 ccgaacccgg gaaaccaaaa tctggcagca caaccttccc cttgccagca aactgctgac
     1921 tgaaaatggg ttttgatgga actgcctggt ttcttgtgga gttgaatgcc ttgtgaggta
     1981 aaagtgaatt agcgatacct ggaacataag aaactgcatg ctcggaaaga aatataagcc
     2041 ggcctaagct ttacaaagct ctctaaaata gctttataag ctcatccgca agattgatcg
     2101 atgttcaatt caactaaaaa caccgacaat tttcggaaat gccggaaagg ccaagaggat
     2161 gatcgcaagc aggttagagg cggctcagac cggcataccg gaagaagttt acgcctaccg
     2221 cacccagcgg taaaacttgt gcctaccaag tcttttacca gggcgctcag ggcgccctga
     2281 gacaatttcg aagccagtca aaacctggcg actgagacat cgtaccgtca ggtgaatcat
     2341 accctcggtc tgcgccagcc gcgctatcaa cctcaggtat gattttcacg gcaaaccgcc
     2401 tgcttgttta catgtgcgga gcaaaaaatc aggccgcttg atgcaaatgc atactatcgt
     2461 cagagccgct tgcacccatg aagaagagac caagttcttc caggaaggca cggcggcctg
     2521 ccagaggaat gttacagcgg cgactcaagt aggtgggact actcagcact gccacagtct
     2581 cgcaaaatgt ctcggtagcg taacgctcca gcatcacccg acaacaattc tggcgctcgg
     2641 accaaccggc agtttccttc agccgtctca gccaggcctc tctgttcctt tgtgcgtagc
     2701 ccgtcacttc atgccacgaa ttcagcgaag aatagggtgg aagaagctca atccaacgct
     2761 gccgaaggct ctccggcaaa agggcgtaga agatatggct gagttcatgc actaaatggg
     2821 gcaggatcca gtcctcctcg agactttcgc tacccatgac aatatgcggt gaaatgccgt
     2881 ctttcgtgta actgcgaaaa tcggcatata tagtggaaga atcgccgttg ggagtgagtc
     2941 cctcgcaggt ggtcatggtc aagcccagtt tcaaaccggc ctgcaaaagc ggcgtagaga
     3001 caaaggggaa aaccgagcgc aaacgctcga tgtgttcagg gctggttccc tcctggtaat
     3061 aaatgccggt gcttccatca aggtagaatc ggcccagcgc atgctggcaa cgtttgttgc
     3121 accaggggcg ctctcttata gtcagcacac gcgaaacagt caggtcttgg ttagcgcagt
     3181 tcatttatgt cgtctccttc aattgcagtt ttctacaaag gctcaggaag agctcaaaca
     3241 ggtttgttca gatagacagg tactggtaat gtcgtgttga gaaaattgga ttccgttgaa
     3301 attttgaaac tgattttcct tcagcttaat cacgtgcagg gggcaaaatc tccctcgtgt
     3361 aagtcattgt gaggctactc tgaccacctg aaggaaaaat ctagatgcta ttgatgcaat
     3421 ttgggctagc gcagctagca ccgcccgcaa taccgcccac gacaccacta caacagcacg
     3481 aaagccgatg agcagcagct gttcatcggc gttttatcca tttgtcgtcg gtgtaaagaa
     3541 ccaagctgtt accagtgcta aggcaggtaa ctcttgcaag caagaagccc ttctggtccg
     3601 acaaacacat attcaattca gtaaactgcg gtcaacggac cgacttgaaa ttattgtccc
     3661 tggcgggaca tatacatcat cggcatgaaa gcccgaaggc aggcttgcaa acaccaggaa
     3721 caaaccgcat cagcagtgca aaaacaaaca ccggcagctt cttcagcacc ggcaaatgca
     3781 cttattgagg attgttcgaa gacggctagc aagttcgttt catgcccaaa gtgatctctt
     3841 gattatatga gaccgacgac tctcaagaag ctgtcgttaa gttttacgcc cgacccttac
     3901 tgcattttca tctgttatgg gctatggaca gccattttca catttcacta tatcttataa
     3961 tatcctcagg caaaaaaaat ggtatgggga cggttcagcg aaccggggcg cacgcccaac
     4021 tc
//
LOCUS       DAOQNZ010000165         4005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10274_length_4005_cov_4.213671, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000165 DAOQNZ010000000
VERSION     DAOQNZ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10274_length_4005_cov_4.213671"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="PKW73_04180"
     CDS             <1..1436
                     /locus_tag="PKW73_04180"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNA72508.1"
                     /translation="GAIYKSVLEKERKGDYLGGTVQMIPHVTNEIKNFLRKGALASKA
                     HVVIVEVGGTVGDIESLIFLEAIRQMRKDVGRDNCCYIHVTMIPNLKTTSELKTKPTQ
                     HSVDTLRSRGIQPDILACRTEKNIGTSIKEKLSLFTDVDPNCVIQCRDVEHLYEVPLC
                     LEEEGLAERVLEKLRLPNTEPDLVAWKEMLERIKHPAKKVTVAIVGKYVMLSDAYISV
                     VESLKHAGAKLHTQVNIKWVLSEDIEKHSAEAYLADVDGILVPGGFGDRGIEGKIAAA
                     GYAREHNIPFLGLCLGMQIAVIEFARTVAKMDKAHSTEFDSGSPFPVIDLMPDQHNVT
                     HKGGTMRLGRYPCVLKAGSIAARVYGQTEISERHRHRYEVNNALRDDLSKHGLIFSGL
                     SPDERLVEMIELPGHPYFVACQFHPELKSRPDQAHPLFVGLVEAMVKEADMKGSANII
                     AAKPEKAEKADSGKPVNPAGKTTTNKA"
     gene            complement(1433..3001)
                     /locus_tag="PKW73_04185"
     CDS             complement(1433..3001)
                     /locus_tag="PKW73_04185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72509.1"
                     /translation="MEEKPLKVNEELSGESSLFLTASSSEPPELPLLGVRYEVQNLLG
                     EGGMGVVWQVFDRELKAVMAVKVLKRALCRDAAAVRRFEQEAKAVSSLKHPSLVTIYG
                     FGTTNDGLPYLSMEYLSGETLAAYLERKGTLDLAQSLNLFCQIAEALAAVHEAGIVHR
                     DLKPGNIFLSQIDGVYRIKLVDFGIAKVQAVEGRDAMTLTQTGEFFGSPLYMSPEQCK
                     GEKIDGRCDIYALGCVMYECLSGVSPFQEQAENQLKLLLAQISSPPAPFHTGLLLPES
                     LKAVVFKCLAKEPSARYSTVQALLSDLERITSGNQEGSYLQATVAKSTTTGGAWSQSI
                     VWIAITVLIFFCLFPRTLPEPLNLMLALLLLSALAYGFYKLFQTDRGLRLEENSYKCH
                     TLADIETVESLISTLPSRGFPLKGIERFWGGFSILLRQNDRSVMQGRCYYPEIYHRDD
                     GSSYESLEFIEMNIDIRAGEKGTDVNVIFSGTRLSSEPAQALKTMTSAHIYQLLSENY
                     LHLSRTDSEKESQS"
     gene            complement(3020..3385)
                     /locus_tag="PKW73_04190"
     CDS             complement(3020..3385)
                     /locus_tag="PKW73_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72510.1"
                     /translation="MNSLRYGIAYDPTGALGKQVAPAMKDLKVATKSVDLKSDGKVLL
                     NDNEGRLKVYVDEKREEGKNWVGISLEQKARLSRLEAVTFDTVTYKIGTLCPAGNTNA
                     ALKELLAEFVKDFQGSRKP"
     gene            complement(3561..>4005)
                     /locus_tag="PKW73_04195"
     CDS             complement(3561..>4005)
                     /locus_tag="PKW73_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HNA72511.1"
                     /translation="NLSGTGANVLSIIMLNPSLADADSDDPTIASCVRLTRNNGYDGL
                     IVANLYAFLTPYPRELFAARDPVGRLYGRNNDAYIELSQRLSRSTLLAWGNQALVDRA
                     RIDSVLKLLKRERCLMLGLTERRQPRHPLYVAATTKMQKASALWG"
BASE COUNT         1020 a         1106 c         1022 g          857 t
ORIGIN      
        1 cgggtgccat atataagagc gtccttgaga aggagaggaa gggcgactat ctgggcggta
       61 ccgtacagat gattccccac gtcaccaatg aaatcaaaaa ctttcttaga aagggcgccc
      121 tggcctccaa agcgcacgtt gtgatcgtag aagtaggcgg caccgtcggc gacatcgaaa
      181 gcctcatctt tctggaagca atcaggcaga tgcgcaagga cgtgggccgg gacaactgct
      241 gctatatcca cgtcaccatg attcccaatc tcaagacaac cagcgaactg aagaccaagc
      301 ctacccagca ttcagtcgac accctgcgca gtcgcggtat tcaaccggat attctggcct
      361 gccgcaccga gaagaacatc ggcacctcca tcaaagaaaa actttctctt ttcaccgacg
      421 tagatcccaa ttgcgtaatt caatgccgtg acgtagaaca cctctacgaa gtaccgcttt
      481 gcctggaaga agaaggactg gctgagcgtg tactggagaa actgcgcctg cccaacaccg
      541 aaccggatct ggtggcatgg aaggaaatgc ttgagcgcat caaacatccg gccaagaaag
      601 ttaccgtggc cattgtcggc aaatacgtca tgctctccga tgcctatatc tcggtagtgg
      661 aatccctcaa acatgccggc gccaaactgc acacccaggt gaacatcaaa tgggtgcttt
      721 cggaagacat cgagaaacac tcggctgaag cctatcttgc cgatgtggac ggcatacttg
      781 tgccgggcgg cttcggcgac cggggcatcg agggcaagat tgccgccgcc ggctacgccc
      841 gcgagcataa tattcccttc ctgggactct gcctgggaat gcaaatcgcc gttatagaat
      901 tcgcccgcac tgtggccaaa atggataagg ctcattccac cgagtttgat agcggaagcc
      961 ccttccccgt cattgacttg atgccggatc agcacaatgt cacccacaag ggcggcacca
     1021 tgcgcctggg ccgctacccc tgcgtactga aggcaggcag catcgccgcc cgcgtttatg
     1081 gacagaccga gatctcggaa agacaccggc accgctatga agtcaacaat gccttgcgag
     1141 atgatctctc caagcacggc cttattttct caggactctc gccggacgag agactggtgg
     1201 aaatgatcga actgcccggt catccgtact ttgtcgcctg ccaattccac cccgagctga
     1261 agagccgccc cgatcaagcg catccgctct ttgtcggact tgtggaagcg atggtaaaag
     1321 aagccgacat gaaaggcagc gccaatataa tcgccgccaa accggaaaag gccgagaagg
     1381 cagattcggg aaagcccgtc aatccagccg gcaagacaac caccaataag gcctaactct
     1441 gagattcctt ttctgaatcg gtcctggaca gatgcaggta gttttcagaa agtaattgat
     1501 aaatatgggc gctggtcatg gttttaagag cctgagccgg ctcagaagat agccttgtgc
     1561 cggaaaaaat cacgtttacg tcggtgcctt tttcaccggc gcgtatatct atattcattt
     1621 caataaattc gagactctca taactgctgc cgtcatcccg gtgataaatt tccggatagt
     1681 agcagcggcc ctgcatcaca ctacgatcgt tttgtcgcag caaaatggaa aaaccacccc
     1741 agaagcgttc tattcccttg aggggaaatc cacgactggg caaggtactt atcagtgact
     1801 ccacggtctc tatgtcggca agtgtatggc acttgtagct gttttcttcc aatcgcagtc
     1861 ctctgtcggt ctgaaagagc ttgtaaaacc cataggccag agcagaaaga agaagcagag
     1921 ccagcatcaa attgagcggc tcaggcaaag tgcgcggaaa caagcaaaag aagattagaa
     1981 cggttatggc aatccagact atcgactgag accaggcacc accggtagta gtcgacttcg
     2041 ccacggttgc ttgcaagtaa ctgccctcct gattgccgga ggtaatccgc tccagatcac
     2101 ttagcaaggc ttgcaccgtg gagtagcgcg cggaaggctc cttggcaagg catttaaaga
     2161 ctactgcctt gagcgactcc ggcaaaagca ggcctgtgtg aaagggcgcc ggcgggctgg
     2221 aaatctgagc cagaagcaac ttcaactggt tctccgcctg ctcctggaaa ggactgactc
     2281 cgctcagaca ctcatacatg acgcaaccaa gggcgtagat atcacatctg ccgtcaattt
     2341 tttcgccctt gcactgctcg ggactcatgt agagaggact accgaagaac tctccggttt
     2401 gagtgagggt catggcatcg cgtccctcta cagcctgcac tttggcaatg ccgaagtcca
     2461 ccagtttgat gcgataaaca ccatcaatct gacttaggaa gatattgccg ggcttgagat
     2521 cgcgatgcac aattccagcc tcatgtacgg cggcaagagc ttcggcgatt tgacaaaaga
     2581 gatttaatga ctgtgccagg tcaagggttc ccttccgctc gagataggca gccagagttt
     2641 ccccgcttaa atactccatg ctcaaataag gcaagccatc attagtggtg ccaaacccgt
     2701 aaatcgttac aagggaagga tgcttgagag atgaaacagc tttcgcttcc tgctcgaagc
     2761 gcctcacagc agccgcgtct cgacaaagag cgcgcttcaa aaccttgacg gccattaccg
     2821 ccttcaactc ccgatcgaag acttgccaaa ctacacccat gccgccttcg ccaagcaagt
     2881 tctgcacttc gtagcggaca ccgagcaagg gcagttccgg aggttcagaa gaggacgcag
     2941 ttaaaaaaag cgacgactcg ccgctcaact cttcattcac cttcagcggt ttctcttcca
     3001 actgatctgc ctcgacaggc tagggcttgc ggctgccctg aaagtctttc acgaattcgg
     3061 ccagcagttc tttcaaggcc gcattggtat taccggccgg acaaagggta ccaattttat
     3121 aggtgaccgt atcaaaggta acagcctcca gacggctcaa gcgagctttc tgctccagag
     3181 agatacccac ccagttttta ccttcctcac gcttctcatc cacatagact ttgaggcgtc
     3241 cctcattgtc attgaggagg actttaccat cgcttttgag atcgactgac tttgtagcca
     3301 ccttcaaatc tttcattgcc ggtgccactt gcttgcccag ggcgccggtg ggatcgtaag
     3361 cgatgccgta tctgagagag ttcaaaccgg tcaaactctt cttttgccac ggctcggcag
     3421 atacctttac ttgcgcctgt gcagcagccg cgaaagcaaa cagcgccagg ctgagtgaac
     3481 acacccgtat agatttcaag acgtttctcc tgaggtaact gtcgtattct acaggaatcg
     3541 aagcaaatta acggagccgg ctagccccac agcgctgagg ctttctgcat tttagtcgtc
     3601 gccgccacat aaaggggatg acggggctgc ctgcgttcgg tcagccccag catcagacag
     3661 cgctccctct tgagcagttt taagacacta tcaatgcgcg ccctatcgac aagagcctgg
     3721 ttgccccagg ctaacagggt tgagcgggag agtctctgac tgagttcgat ataggcatcg
     3781 ttgttgcgac cgtaaagacg tcccaccggg tcacgagccg caaacaattc tcgcggatag
     3841 ggcgtcagaa aagcgtaaag attggctact atcaagccgt cataaccatt gttgcgggtg
     3901 aggcgcacac agctggcaat agtcggatca tcagaatcgg catcagccag ggatggattg
     3961 agcatgatga tggagaggac gtttgcaccg gtgccggaaa gattt
//
LOCUS       DAOQNZ010000166         4001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10295_length_4001_cov_3.599088, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000166 DAOQNZ010000000
VERSION     DAOQNZ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10295_length_4001_cov_3.599088"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PKW73_04200"
     CDS             <1..1019
                     /locus_tag="PKW73_04200"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNA72512.1"
                     /translation="YALPSNKLKKGEIESKVMLGEPIVIGRDNEGKLFALRDICPHQA
                     VPLSAGPFDGKELMCPFHGWKFDTSGTCTEVPSLCSDQKMNLCKIRTANYPVKEVLDS
                     IWVYFGDTVNEADLPEVPYAPGLDGLKYRKNTVTLQLPTHIDYAVAALIDTAHVPYVH
                     KSWWWRSAKSVKEKTKTYVPSGTGWTMVKHQPSKHSLAFKLIGKYLETEISFRLPGCR
                     REYLTFNGTTFLAGITTLTPVDETHTELNHTTYWTLPIPKIVANPVLDYFVTTFLKQD
                     QTLALMQENIINKYKPRLIMTIKDAGTPGHWYFLLKKEWNAATSEKRPFVNPIKETKL
                     RWKT"
     gene            1191..2012
                     /locus_tag="PKW73_04205"
     CDS             1191..2012
                     /locus_tag="PKW73_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72513.1"
                     /translation="MFEQSERQVKALGTPVNAGDLSADIYSPSENRSWQFLKTQAEAP
                     EKPEAKEQRDILQGLLSLPKDKLSAGLSITADAAREFADSPDKKAALEKTTPKFQAGM
                     EQSDKDFGKAMGDNWSTLLSARREVTTVQSLSMRENMDAAAKAKEIPEDKRRSVMGMS
                     ALLLDDAVPRETKEHLRGALKDYPGMLQSVDNLLKLKERGEKAQEAMEAAQKPLLDAA
                     MSQAATRFVYAKALEQGKDIGSSLRVKQEGEEIWQRALSKITGAPMPEVKPALKV"
     gene            2290..3546
                     /locus_tag="PKW73_04210"
     CDS             2290..3546
                     /locus_tag="PKW73_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72514.1"
                     /translation="MANEVATAGDKQPVQGDDNTASDAKAKLTAEANLASPKPVSSAQ
                     SCQGHEGMCHPGKGDNAVISNDQIINFSPPEKLVHDTAGIKIKEEDPGKLEANAIKNT
                     KDLFLNPATSQEDRVRAAQALVRNGINEVQGTDGKTYQLNSVKEGEREQISVTTLKAG
                     EAPSVELKGALNAQGEVLEVGGAEQPKKSETEAPPVKPQEEIPPYSGKAIFNPALSNE
                     EKLLEADRMFKSGEKRFLGPDGKAYETSETQVGDRKVISIFTKDENGAARPLLRGIID
                     KSGAMANQQDDRFKAVDYESDYAKKHGNETALLNREAELKRRQEAEKRRQEEEAEKQK
                     QEAQRKEQEEAAKLKQQQEEAERKRKEEEERNKKPATAEEQLLKTADEKFKSETDKSE
                     FKNDMDDFQNRARARNLPQQEIDKTY"
     gene            3562..>4001
                     /locus_tag="PKW73_04215"
     CDS             3562..>4001
                     /locus_tag="PKW73_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72515.1"
                     /translation="MQEGSAAVPEEHRQLAAKTLMHQLAVPNITDQGYHNTCNVTTLA
                     ERTLTRQPAMAAEIVATSAIKGSWTARDGKEIKFKAQDLVPGSEEKFQHRTSDGSRTY
                     ATQLLNLAMVNDSLQRKMPPQYYGYGSPSATNKTGETVTYADGSP"
BASE COUNT         1194 a         1111 c         1002 g          694 t
ORIGIN      
        1 attacgccct cccttccaac aaactcaaga agggtgaaat cgaaagcaaa gtaatgctgg
       61 gcgaacccat cgtcatcgga cgcgacaatg agggcaaact ctttgccctg agagatatct
      121 gcccgcacca ggccgtcccc ctttccgccg gtccctttga cggcaaagaa ctgatgtgtc
      181 ctttccacgg ctggaagttt gatacttccg gcacctgtac ggaagttccg tccctgtgct
      241 cggaccaaaa aatgaacctg tgcaagattc gcactgccaa ctacccggtc aaggaagtac
      301 tggacagtat ctgggtctat ttcggcgata ccgtcaatga ggcggacctg ccggaagtac
      361 cttacgcacc cggtcttgat ggattgaagt atcgcaagaa caccgtcacc ctgcagttgc
      421 ctacccatat agattatgca gtggcggccc ttatagacac ggcccacgtg ccctatgtgc
      481 ataagtcgtg gtggtggcgt tccgccaaat cggtgaaaga gaaaaccaaa acctatgtgc
      541 cctccggcac aggctggacc atggtcaaac atcagccttc caagcattcc ttagccttca
      601 aactgatagg caagtatctg gaaaccgaga ttagcttccg cctgccggga tgccgccgcg
      661 aatacctgac cttcaacggc accaccttcc tggccggtat caccactctc acacccgtgg
      721 atgaaaccca tacggagctg aatcacacca cttactggac cctgcccatt cccaagattg
      781 tggccaatcc tgtgctcgac tactttgtca ccaccttcct caagcaggat cagaccctgg
      841 ccctcatgca ggaaaacatc atcaacaaat acaagccgcg tctgattatg accatcaagg
      901 atgccggcac accggggcac tggtacttct tgctcaagaa ggaatggaac gctgccacaa
      961 gcgagaagcg ccccttcgtc aacccgatca aagaaaccaa gttgcgctgg aagacataag
     1021 ctgcactggt accggcaatt aactaagcct tgaggcatca gtcttaagcg catttaagct
     1081 tcgtgtccgg agcagtcgtg atctcaccat agccttataa gctctactta aagataatct
     1141 tgacaactga caaggtcagc accccctaat aaaacctttg aggcacgagc atgttcgaac
     1201 agagcgagcg tcaggtcaag gctcttggca caccggtgaa cgccggcgat ttgagtgccg
     1261 acatttacag tccttccgaa aatcggtcct ggcagtttct taaaacccag gctgaagcac
     1321 ccgaaaagcc tgaagccaaa gaacaacgag acattttgca ggggctcctc tctctgccga
     1381 aggacaaact gagcgccgga ctgagcataa cagcggacgc cgccagagag tttgccgact
     1441 cccccgataa aaaagcagcc ctggaaaaga cgacaccaaa atttcaggcc ggcatggaac
     1501 agtcggacaa agacttcggc aaagccatgg gcgataactg gtcgactctc cttagcgccc
     1561 gccgagaagt gaccaccgta caatcgctct ccatgcgtga gaatatggac gctgccgcca
     1621 aggccaaaga aattccggaa gacaaaaggc gcagtgtgat gggcatgtcg gccctgcttc
     1681 tcgatgacgc cgtgcccaga gaaaccaaag aacatctgcg cggtgccctg aaagactatc
     1741 cgggcatgct gcagtcagtg gacaatttgc tcaaattgaa agaacggggc gaaaaggccc
     1801 aggaagccat ggaagcggca cagaaaccgc tcttagacgc agccatgagc caggccgcca
     1861 ctcgttttgt ttatgctaaa gcgctggaac agggcaaaga tatcggcagc agcctgagag
     1921 tcaagcaaga aggcgaagag atatggcaga gagcgctctc taagattacc ggtgccccca
     1981 tgccggaagt aaaaccagcc ctcaaggtct gatcgaaatc atccagtaaa aacaaggcca
     2041 cagcggcatg gtgtgggaat cctacgaagc gcagataaaa ttcctggcga cgatcgggga
     2101 tttcgtgtta gcttaattga agcaatgcta ttgtctcgat cccctagcag acttccggca
     2161 acgcctcgaa aaaaagacga caaccagcta ccgcatacat agatcaatcc gagcagaatc
     2221 tatgttttcc aaggaagcac gagcaaaccg ctctcatgtg ctccatttcc gctcgggaga
     2281 atttctaata tggctaacga agtggcaacc gccggtgata aacaaccggt acaaggtgac
     2341 gacaatacag cctcagatgc aaaagcaaaa ttaactgcag aagccaatct ggcctctccc
     2401 aaaccggtga gcagtgcgca atcttgtcag ggtcacgaag gcatgtgtca tccaggcaag
     2461 ggtgacaatg ccgttatctc gaacgatcag ataatcaact tcagtccgcc tgaaaaactg
     2521 gtgcatgata ccgccggtat caagataaaa gaagaagatc ccggcaagct ggaagctaat
     2581 gccatcaaga acaccaaaga tctctttctg aaccccgcca ccagccagga agaccgcgtg
     2641 agagcagccc aggccctggt cagaaacggc atcaacgagg tgcaaggtac agacggcaaa
     2701 acctatcaac tgaactccgt caaagaagga gagcgggagc agatttcggt aactactctc
     2761 aaggccggag aggctccttc cgtagaactg aaaggagccc tgaacgctca gggtgaggtg
     2821 ctcgaggttg gtggtgccga gcagccaaaa aagtccgaga ctgaagcgcc gccggttaaa
     2881 ccccaggaag aaattcctcc ctatagcggc aaagccattt tcaatccggc tctgtccaac
     2941 gaagagaaat tactggaagc agaccgcatg ttcaaaagcg gcgagaaacg cttccttgga
     3001 ccggacggca aggcttacga aacctcggaa acccaggtcg gtgaccggaa agtaatatcc
     3061 attttcacaa aagacgaaaa cggagcggct cgtccgcttc tgcgcgggat tatcgacaaa
     3121 agcggcgcca tggccaatca gcaagacgac cgcttcaaag ccgtagacta cgaaagcgat
     3181 tacgccaaga aacacggcaa tgaaactgcc ctcttaaatc gggaagcgga attgaagaga
     3241 cggcaagagg cggaaaagag acggcaagag gaagaagcgg aaaagcagaa gcaagaagcc
     3301 cagcgcaaag agcaggaaga ggccgctaaa ctcaagcagc aacaagagga agcagaaaga
     3361 aagagaaaag aagaggaaga acgcaacaag aagcctgcca ccgcagaaga gcaattgctc
     3421 aaaactgccg atgaaaagtt caagtcggaa accgacaaga gcgaattcaa aaatgatatg
     3481 gatgatttcc agaaccgcgc cagagcaaga aacttaccgc aacaggaaat cgacaaaacc
     3541 tattgacagg taaatcggct cttgcaggaa ggtagcgccg ccgtgccgga agagcacaga
     3601 cagcttgccg ccaagaccct gatgcaccag ttggccgtac cgaatataac cgaccagggc
     3661 taccacaata cttgcaatgt cacaactctt gccgagcgaa ccctcaccag acagccggct
     3721 atggccgccg agatagtagc caccagtgcc atcaaaggtt catggacagc cagagacggt
     3781 aaagagatca aattcaaggc gcaagatctc gtgcccggca gcgaagaaaa attccaacac
     3841 cgcaccagtg acggctctcg cacctatgcc actcagttgc tcaatctagc catggtcaac
     3901 gacagcctgc agcggaaaat gccgccgcaa tactatggct acggcagccc ctctgccaca
     3961 aacaaaaccg gcgaaaccgt cacttacgcc gacggctcgc c
//
LOCUS       DAOQNZ010000167         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10303_length_3999_cov_5.391988, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000167 DAOQNZ010000000
VERSION     DAOQNZ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10303_length_3999_cov_5.391988"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /gene="istA"
                     /locus_tag="PKW73_04220"
     CDS             complement(<1..1604)
                     /gene="istA"
                     /locus_tag="PKW73_04220"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNA72516.1"
                     /translation="MRKIREVLRLRHANGFSQKDIAKTVNCSHGAVGEYLKRASAAGL
                     TWPLPPELELSDDALDKMLYPRAAPSSAVRRPQPNCIQMREELKKKGVSLYLLWTEYL
                     DENPSGYSLSQFCDIYKRFNKNLAITMRIEHKGGDKAFSDFAGTTLPIVSERTGEVTS
                     AHLFVCTLGASSYTFAKLYPDETTSSWCNGHADAFSFFGGSPVVVVPDNPKAVITKAS
                     KYEPDINPSFAQMAAHFNVVVLPARIRKPKDKAKVEAAVGLATRWILARLRKRTFFSL
                     AEANQAVAELLTGLNLKKFQKRPGSRMSLFEELDKPVLRSLPHSKYEYFESKRVAVNI
                     DYHFVFEDHMYSVPCQHRFAKVDLRVTETTIEVFLDGTRIASHIRAFAPGKFSTLPEH
                     RPREHQEYGNWSPSAMLEKGLKIGASTAKLFSAIMASREVPEQGFRSCMGILRLEKQY
                     DRERLEAACARALAINGLSYKSVSSILKSKLESSPLPEKPVQLTVLHSHIRGSQSFAS
                     NIKENNNADTPNDRQPEDSKITGDGQ"
     gene            1714..2382
                     /locus_tag="PKW73_04225"
     CDS             1714..2382
                     /locus_tag="PKW73_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72517.1"
                     /translation="MQLAWCLFAQKLAILDRNPWPFWTGNRGHFPPETMAIFVRNTQK
                     FNYHGFCGYFSLAEVGAAREFSRRALHLEKQQIIALAGAPSFEGRGFNGWEWLSPKSE
                     FWVYNLGGGWIPVYLVFEGNTCVRSGICSRDQFHAYEEWRIRQICQFGPGKSYSSIVS
                     WLGQPDSVSDAKGRKATSPKTPAKQIVSCFYQFSSGTGANIYFKNGICTGSDCGEERV
                     YILH"
     gene            complement(2390..2806)
                     /locus_tag="PKW73_04230"
     CDS             complement(2390..2806)
                     /locus_tag="PKW73_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72518.1"
                     /translation="MISKKYKKLIENFPLVPIRSDAHLDEAHDIAQSLLCRKEPLAID
                     EKEYLEVLLNEISKYEQKHHALSFSELKPHELLQSFMKEHGLKQVDIQTILGISSSGV
                     MSEIVSGKREITKEQCVKLGHHFKVTPAAFLPKIFA"
     gene            complement(2806..3090)
                     /locus_tag="PKW73_04235"
     CDS             complement(2806..3090)
                     /locus_tag="PKW73_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNA72519.1"
                     /translation="MEILNSRAIKQFVREHPDAEAPLREWVAKTRAAEWKNNSDVLKT
                     FRSADHLGDQKFIFNIGGNNFRMAAMVWIDKERVYVLKLMTHAEYDRETF"
     gene            3307..>3999
                     /locus_tag="PKW73_04240"
     CDS             3307..>3999
                     /locus_tag="PKW73_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72520.1"
                     /translation="MPLFFKNKAKEFDKLLTKAQKQNSEKRYKEALESLSKAEELLAG
                     EEAQAHQWAWIYDARRYAQYELGQVDEALNTCRKAIEHLSNTQLFAYLSEDSHVRGTL
                     RSAHNMLAWTLCERAAGEAECQVALEHINKCMATTSPIDDEYQLRPFYETQAIVLLRM
                     SELAKDPTSYKTQLFTVLTKMKKKSHEALTENAKLAEICSSPEFEAHFADDPEAKLKV
                     APPGETVDQALAR"
BASE COUNT          965 a          949 c         1019 g         1066 t
ORIGIN      
        1 tgctggccat cgccagtaat tttagagtct tcaggttgtc tatcgttggg tgtatcagca
       61 ttattgttct ccttgatgtt tgaggcgaaa gattgggaac cgcgaatgtg ggaatgcaag
      121 acggtgagtt ggacgggttt ttctggaaga gggctagatt cgagtttgga cttgagaatt
      181 gaagagacag atttgtagga gagtccattg attgctagag cgcgagcaca ggcggcttcc
      241 aagcgttctc tgtcgtattg tttttcaagg cgcagtatgc ccatacagct tcggaatcct
      301 tgttccggga cctctctaga agccatgatt gcagagaaga gcttggcagt agaggcgcct
      361 attttcaggc ccttttcgag catcgcagaa ggggaccagt tcccgtactc ttggtgttca
      421 cggggtctgt gttccggcaa tgtagagaat ttgccaggcg caaatgctcg aatgtggctg
      481 gcaatgcggg taccgtcaag gaacacttca atggttgtct ccgtgacgcg aagatcaacc
      541 ttggcaaagc ggtgctggca aggcacgcta tacatgtggt cctcaaagac aaagtgatag
      601 tcgatattca ctgcgaccct tttgctctca aagtattcgt atttagagtg aggtagggag
      661 cgaagaacag gcttgtcaag ctcttcaaaa agagacattc gactgccagg acgtttttga
      721 aacttcttta aatttagtcc agtcaaaagc tcggctacag cttggtttgc ctccgccagg
      781 gaaaagaaag tacgtttcct tagtcttgca agaatccatc gtgtagccag tccaacagcg
      841 gcttctacct tcgctttgtc ttttggtttc ctgattctag ccgggagtac aacgacgttg
      901 aagtgagcgg ccatctgagc gaagctggga ttgatatctg gctcatattt actggcttta
      961 gtgatgacgg ccttaggatt atcagggacg acaacaaccg gggagccgcc aaaaaagctg
     1021 aatgcatcgg catggccgtt gcaccaggac gaagtggtct cgtcagggta gagcttagca
     1081 aaggtgtagc tgctcgctcc gagagtgcat acgaagaggt gtgctgaagt gacctcgccg
     1141 gttcgctctg agacaatcgg cagtgtcgtg ccggcaaagt cggaaaacgc cttgtctccg
     1201 cctttgtgtt caatacgcat tgtgattgcc aggttcttgt tgaatcgttt gtagatatcg
     1261 cagaactgac tcaagctgta tccagaaggg ttttcatcaa gatattcggt ccaaagtagg
     1321 tagagtgaga cgcccttctt ctttagctcc tcacgcatct gaatgcagtt tggctgcgga
     1381 cggcggactg ccgaactagg cgcagctcgg ggatacagca ttttatcaag agcgtcatcg
     1441 cttaactcca gctctggagg aagcggccag gttagtccag ctgctgatgc ccgcttcaag
     1501 tattccccaa ctgcaccatg cgagcaattt actgtcttag ctatgtcttt ttgactaaaa
     1561 ccgttagcat gacgcagtct tagcacctct ctaatctttc tcatggacat tctccgattc
     1621 ggcattggca cctcattagc tagctagcaa acaaagtgtt actgctcagt aaaatgtcca
     1681 gcgtcaatca agccagctgc atatggttcg cgtatgcaat tggcttggtg tttattcgct
     1741 cagaaattgg ccattttgga ccggaatcca tggccatttt ggaccggaaa tcgcggccat
     1801 tttccgccgg aaaccatggc cattttcgtc cggaatacac agaagtttaa ctatcatgga
     1861 ttttgcggtt actttagtct ggctgaggtc ggcgctgcca gagagttttc ccggagagcg
     1921 cttcatcttg agaagcagca gattattgcg ctggctgggg caccgtcatt tgaggggcgc
     1981 ggttttaacg gctgggaatg gctcagccca aaatccgaat tctgggtcta caatcttggt
     2041 ggtgggtgga taccggtcta tcttgtgttt gagggtaaca cctgtgttcg ctccggtatc
     2101 tgcagcaggg accagtttca tgcctatgaa gagtggcgaa tacggcagat ttgccaattt
     2161 ggtcccggta agtcttattc gtccattgtc tcatggcttg gccagcccga ttcggtttct
     2221 gacgctaaag gtagaaaggc aacgtcgccg aagacacccg caaaacaaat cgtttcctgc
     2281 ttctatcaat tcagtagcgg aaccggtgcg aatatctatt tcaaaaatgg tatctgtacc
     2341 ggttctgact gcggtgaaga gagggtttat atattgcact gatcaattat caggcgaaaa
     2401 tctttggaag aaacgctgcc ggtgtaactt tgaagtgatg acccagtttg acgcattgct
     2461 ctttagttat ttctcgtttg cccgacacaa tctcactcat cacaccggaa gaagaaattc
     2521 ccaggatggt ttgaatgtct acctgcttga gcccatgctc tttcatgaat gattgtagaa
     2581 gctcgtgagg cttgagttcg ctgaacgaaa gggcgtgatg cttttgctca tacttgctga
     2641 tttcattcag caaaacttcc agatattcct tctcatcaat ggcgagcggc tccttcctgc
     2701 atagcaggct ctgagcaata tcgtgggcct catccaggtg agcatcgctc ctgattggta
     2761 ccagtgggaa gttttctatg agcttcttgt atttcttgct tatcatcaga aggtttccct
     2821 gtcgtattca gcgtgtgtca tgagcttcaa aacgtagact ctctctttgt ctatccatac
     2881 cattgccgcc attcggaaat tgttgccgcc gatattgaaa ataaatttct ggtctcccag
     2941 atgatcagcg ctcctgaagg ttttcagaac gtccgaattg tttttccatt cggcagctcg
     3001 cgtttttgcc acccattctc tgagcggcgc ttctgcatca ggatgctccc gaacaaactg
     3061 cttaattgct ctggagttga gtatctccac gatttgcacc ttccctcaat tctatcctct
     3121 caaaaagaga ggaagaacgc aataccgtgc ctctcaattt gagaggattt aatcttcgat
     3181 tgggatcaac cctgagctgt gagaagctaa agtagccggg gataccggag tttcaatttc
     3241 ccaccggcta aaaaatcgcc cctgtgcgta agcaatttat tgaacccgtt tgaaagagag
     3301 tcttttatgc ccttgttctt caagaataaa gccaaagagt tcgacaagct tctgacaaaa
     3361 gcccagaagc aaaattcaga gaaacgttat aaggaagccc ttgagtccct ctctaaggca
     3421 gaagagctgc ttgccggaga agaggctcag gcgcaccagt gggcctggat ttatgatgca
     3481 agacgctacg cccagtatga actggggcaa gtcgatgaag ccctgaatac atgccgcaaa
     3541 gcaattgagc atctaagcaa tacccagttg ttcgcctacc tgagcgaaga cagtcacgtg
     3601 cgcggcacgc tccgctctgc ccataacatg cttgcctgga cgctttgtga gcgcgccgcc
     3661 ggtgaggctg aatgccaggt tgccctggag catattaaca agtgcatggc caccacctcg
     3721 cccattgacg acgagtatca gctcagacca ttttacgaaa cccaggctat tgttcttctg
     3781 cgcatgagcg aattggctaa ggaccctacc agctacaaaa ctcagctttt taccgttttg
     3841 acaaaaatga aaaagaagag ccacgaggcg ctcactgaga atgccaaact ggcagagatt
     3901 tgcagttcgc cagaatttga ggcgcacttc gccgatgatc cggaggctaa actgaaagtg
     3961 gctccgcccg gtgaaaccgt tgaccaggct ctagccagg
//
LOCUS       DAOQNZ010000168         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10317_length_3997_cov_3.589802, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000168 DAOQNZ010000000
VERSION     DAOQNZ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10317_length_3997_cov_3.589802"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKW73_04245"
     CDS             <1..866
                     /locus_tag="PKW73_04245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA72521.1"
                     /translation="SNALRQIAAEYDGVKLIELSRNFGQQAAITCGLDHSEGQVVVNI
                     DSDLQDPPELIVDMLQKWKEGYDVVYAQRSTRRDRFLKRFTAYLFYRLLGAVSSVEIP
                     WDTGDFRLMDRKVVLALASLPEKTRFLRGMIPWLGFKQCGISIDRGAREMGESTYTIK
                     KLISLALDGLLALSIAPLYFISVAGGVIIGLSVLSLVVALAAGSFMSATLQSQLVIVS
                     CIALFSGVQIFCTGVVAAYMAKVLDETRARPTYIVGNRLGQGFESGSRSKVATVSGRQ
                     MEKAEFAAGHR"
     gene            1719..2333
                     /locus_tag="PKW73_04250"
     CDS             1719..2333
                     /locus_tag="PKW73_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72522.1"
                     /translation="MSVKAMLREYIGDLPEDQLFCTRDLLGLCSRQSLDVALSDLVKE
                     EFLERLAQGVFRLRKAENAPVSPLVVAEVKLKAFGKSMFDLPEERRSPVSRKLVSAET
                     ESSLVIVGTDGKSSSFRYQGKKIVCLSLGPRKCRLGNGLFGSTLRRLWLLGSGEIPKE
                     QAQPAIDNLLMNQQLWNKFVKFLPDWLCRQLSMAGLAYRPAQVV"
     gene            complement(2335..3726)
                     /locus_tag="PKW73_04255"
     CDS             complement(2335..3726)
                     /locus_tag="PKW73_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNA72523.1"
                     /translation="MSGNHGDKKKLFGAYDIRGIYGDGLDEVFARKVGKAYGSYLKPQ
                     GGGKVLVGHDARLSSPSLHKAFVEGILASGNDVVDIGLSSTPMAVWHAAHAGMDGGAV
                     ITASHLGKAYNGFKLYAAKAKPLGANNGLGEIRKVVENTVVEDGNSAQTPEPKASAAG
                     KVIPATVTEHYVTSLMEHLKPKRRLKIAIDAGHGAAGPEIIEFAKQARELVEIITLNA
                     EPDGAFPKRTPNPLDEGSLDELIETVKGGNCDFGVSLDGDADRAVFVDELGNLVPTDL
                     MIGLIGGELIKRHGGGSVVYDLRASRAVPEYIAEAGGTPIRTGVGTNFMMNSIKDNNA
                     LFAGELSGHYYYGDMFATDNALRTILEAINVVSGNEAALSSLIQPLCRYALSGEINLK
                     VQEIQSTLQKLETTVTGGTKEHIDGLSINFNEWWFAARGSQTEPVLRLTVGAVSEAIL
                     EDRTKQLIEIIQN"
BASE COUNT          895 a          934 c         1083 g         1085 t
ORIGIN      
        1 cctccaatgc tctccggcag attgccgccg agtacgacgg tgtaaaacta atcgagctct
       61 cccgtaattt cggacaacag gccgctatca cttgtgggtt ggaccacagc gaggggcagg
      121 ttgtggtgaa tattgattcc gacttgcaag atccgcctga gctgatagtg gatatgcttc
      181 agaagtggaa agagggctat gacgtagtct atgcccagcg ctcaactcgt agagatagat
      241 ttctaaagag attcactgcg tatctgttct ataggctgct cggtgctgtt tcttccgttg
      301 aaattccctg ggataccggt gatttccgct tgatggacag gaaggtcgtt ctggcgcttg
      361 cttcgctgcc ggaaaagacc agattcttgc gtggcatgat tccctggttg ggctttaagc
      421 agtgcggtat ttctatcgat cgcggcgcca gagaaatggg tgaaagcact tatacgatca
      481 agaagttgat cagtctggca ctggatggac ttttggcttt gagtatcgcc cccctgtatt
      541 ttatttctgt agcgggcggg gtgataatcg gcctttcggt tctgtcgctg gtggtggctc
      601 ttgccgccgg tagcttcatg agtgccactc tacagtcgca attggtgatc gtttcgtgta
      661 tcgccctctt ctccggggtg cagatttttt gcaccggtgt ggttgctgcc tatatggcaa
      721 aagttctgga tgaaacccgg gcacgcccca cttacatagt tggtaaccgc ctgggtcaag
      781 gcttcgagtc cggcagtcgt agcaaggttg ctactgtcag tggccgacag atggaaaaag
      841 cggagtttgc agccggtcat cgatgagatt gattggtttt cagatttttc caagtaattt
      901 ctaaaggtcg tctggactta gtgcagtcaa ccctgtgctg ccctttgacc gtattcgggc
      961 gcctgccggt ccaggagtag agataaatta ccgttgtcgt gagaagagtg ggagagttag
     1021 caactacctc tcgctgtttc tatgatgctg gggttgattt agaaaaacag cggaaaatct
     1081 aaaataggcg ccaggtggct ctgcggttgg cgcctatctt agtccggtac ctggtgtggt
     1141 ggatgagcta aggtgaattt agaaaaacag cagaaaaact aaattcgagt gttggtcctg
     1201 gtgttgtacc aaaagggttg gagaggagtc cacatcaatc agggaccttc tttagccagt
     1261 ttgcgatgtt gcgattgatt tagaaaaaca gcggaaaatc taaattcgat tgtcgggcct
     1321 ggtgttgtac caaaagggtt ggagaggagc ccacatcaat cagggattgt ttagccagtt
     1381 tgcgatgttg aggttgattt agaaaaacag cggaaaatct aaaattggcg ccaggtgtct
     1441 ctgccgttgg agaaaaatct gaatccgata cccggtgtgg ctaagtcgcg agggtgaatt
     1501 aagaaaagca gcggaataac taaattcgtt tatcgtcctg gcgctgtgaa gatacggtta
     1561 gaagggagtc caggtcaatc cagttggctg agcactgtgt tttagaaaaa cagtagaaga
     1621 tctgaaccgc ccccaatagt ttttaacttc tccgtttgaa ccaaatgtga tctgctgccg
     1681 ttttagtagg taggagaatt tggcacgggg ggcgcattat gagcgttaaa gcaatgttgc
     1741 gtgaatacat aggcgattta cccgaagacc agctgttttg tacgagggat ctgctggggc
     1801 tttgcagccg acagtcactt gatgtagctc tttccgacct cgtcaaggaa gaattccttg
     1861 aacggctcgc acagggagtc tttcgcttga gaaaggcaga gaatgcaccg gtcagcccac
     1921 tggtggtggc agaagtgaaa cttaaagcct tcggcaagag tatgtttgat ttgcctgaag
     1981 agcgccgcag tcctgtaagc agaaagctag tatcagccga aactgagagc agcctggtga
     2041 tagtgggcac agacggtaag tccagttctt ttcgctatca gggcaaaaaa attgtatgcc
     2101 tctcccttgg cccgcgtaag tgccgcctgg gcaatggtct cttcggttcg actctgcgcc
     2161 gactctggct cctgggaagt ggagaaattc ccaaagagca agcacagccg gcaatagata
     2221 acttactgat gaaccagcaa ctttggaata agttcgtgaa gttcttgccc gattggctct
     2281 gtagacagct atctatggca ggtctggcgt atcgtccggc tcaagttgtc tgagttagtt
     2341 ctgaataatt tcaatcagtt gctttgtgcg gtcttccaga attgcttccg ataccgctcc
     2401 caccgtcagt cttaatacag gttctgtctg ggagccgcgg gctgcaaacc accattcgtt
     2461 gaagttgatg gaaagaccgt cgatgtgctc ttttgtgccg ccggtgacgg tggtttcgag
     2521 cttctgcaaa gtgctttgaa tttcctgcac tttcaggttt atctctccgc tcaaggcgta
     2581 acggcagagc ggctgaatca aactagaaag ggctgcctcg ttgccggaaa caacattgat
     2641 tgcttccagt atggttctga gcgcgttgtc ggtggcgaac atgtcgccgt aatagtagtg
     2701 acccgacagt tctccagcaa agagagcgtt gttgtccttg atgctgttca tcatgaagtt
     2761 ggtaccaaca ccggtgcgaa tcggtgtgcc gccggcctcg gcgatgtact ccggcacggc
     2821 ccgactggct cgcaggtcgt aaactacgct gcctccgccg tggcgtttga taagttcgcc
     2881 gccaatcaga ccgatcatca aatcggtggg caccagattg cccagttcgt ctacgaatac
     2941 ggctctgtct gcgtctccat cgagggatac cccgaagtca cagtttccac cttttacagt
     3001 ttcaattagt tcatcaaggc tgccttcatc caggggatta ggcgtacgtt tggggaaagc
     3061 gccgtccggt tcggcattga gggtgattat ctcaacaagc tctctggcct gtttggcgaa
     3121 ctctataatt tccggtccgg cagcaccatg tccggcatcg atggcgattt ttagtctgcg
     3181 cttgggtttc aaatgttcca tcaggcttgt cacgtaatgt tccgtgactg tcgccgggat
     3241 tacttttccc gccgcacttg ctttgggctc cggagtttga gcagaattgc cgtcttcgac
     3301 tactgtattt tctacaactt ttcgaatttc acccagtccg ttattggctc cgaggggctt
     3361 agcctttgct gcatagagct tgaagccgtt gtaggctttg ccgagatgag aagccgttat
     3421 tacggcaccg ccgtccatac cggcatgggc cgcatgccag acagccatgg gagtggaaga
     3481 aagtccgatg tctactacat catttccact tgcgagaatg ccttctacaa atgctttgtg
     3541 tagggatggc gaagaaagtc gggcgtcgtg accgacaagt accttgccgc caccctgcgg
     3601 tttgagatag ctaccgtagg ccttgcccac cttcctggcg aagacctcgt ctaaaccgtc
     3661 tccgtatatg cctctgatat cgtacgcgcc aaaaagtttt ttcttgtcgc cgtgatttcc
     3721 actcattagt aacagctctc cgccatcagt ttgcctggga tttcctggtt ggataggcag
     3781 tttatatgcc tctgtgccgg tctggatact tttgttatag cgcttccggc ttcacttctt
     3841 tactcgattg ataatacaac tttgccggtc ttaagtgctg tacctctaca ttacgtcagc
     3901 gaagctcttc ttggttttat caaagaattt gaaaccaaga atagcaacaa caacagctag
     3961 agccgtgtaa cttgcatagt gaggccagtc cggcggg
//
LOCUS       DAOQNZ010000169         3996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10323_length_3996_cov_3.640700, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000169 DAOQNZ010000000
VERSION     DAOQNZ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10323_length_3996_cov_3.640700"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..730)
                     /locus_tag="PKW73_04260"
     CDS             complement(116..730)
                     /locus_tag="PKW73_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72524.1"
                     /translation="MQTIQQSISDSMRVLEKWTEAQNSNTTAVSTEEMHATMKELLAN
                     DLAARGFMAALSTSPIDLSGNFLKALRQAVQEHPAVSHDLIVKNIIMSATASIQHKNS
                     GKTEQALASQAVSERNCRLAQLLSNEDLDATLKAASKAIDSFEMTRADTATAASAAGA
                     SNQTVATSATGQTDDYWHNFFRRWAYTRELLLEVRSRYSSSFSL"
     gene            complement(743..1669)
                     /locus_tag="PKW73_04265"
     CDS             complement(743..1669)
                     /locus_tag="PKW73_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72525.1"
                     /translation="MINTRQSEFSTVFFKMRLVLILAIGCILIGATYAWSNNLGGQQT
                     EPDPLAQPLPAAVRKAKNRLPAVLGGMDTALRAQATPAELVPALAVANADGIVSRKPE
                     QLAQSEVRGPETVTSIESARQSAKAALGIRDPFEPLGQLQSFPRLPAGAENQPTVKDK
                     NGKLVKTAIPGKNVPQLELVPPPPTGSGLEQDMSAMPGPHNGYMPPPLPGGMSGGGLP
                     SGLALSDLPMPPEKPSVLRYLRLSGIIGDKAIFQVKDPLVRRANHWPEQITLAPGESF
                     GTAHLVSVSARDEKAVMEEHGQMEELDLPSIR"
     gene            complement(1680..2420)
                     /locus_tag="PKW73_04270"
     CDS             complement(1680..2420)
                     /locus_tag="PKW73_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72526.1"
                     /translation="MNRRTRMLIVFGPLAVSATGALALTYPAFLDSQHEHEELAKQEI
                     ELQEVKGKLTDKASADASHKALQEDINSLRNEVPHAPYLDLLLLDLERMALASRVDII
                     SVEKQDEKGANNQTPADGADLEVLEAAGMKDRKVNLPAPLQNLSNQVQGQGQNKAKDE
                     NKGPEQNNMGVKQLSRRVYITGDYNSLIDFMKRMEAYQRVLSVNSLSIALASSPNGQN
                     KSAAGEKAQKLKLSQPVLSFMLNIYYLP"
     gene            complement(2417..>3996)
                     /gene="pilM"
                     /locus_tag="PKW73_04275"
     CDS             complement(2417..>3996)
                     /gene="pilM"
                     /locus_tag="PKW73_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNA72527.1"
                     /translation="SGVPTGGQSPSVNICVPAQGVVIRLMPVPVGMPPEELADVVTQE
                     ATNHVPFPISEANLDWCQMNATERTDPDGVRRVDVILAAIQHSIVESYWRMADAAGVK
                     LGRVEVSSLSVIRALALAGYLGSSGHLSMVVNIRQDATDINIVRSAMPLFGRSIILGV
                     EALTESVARSLDISFDQAMELLPEIPVFNITPTDARMGQAGQVARTIFSDIGDEVMRS
                     LEFYKSQVGDVKIDQVLLTGPGCMLPNMQEYFSSKLRMKTILGDPMRDMTVDDRFISS
                     RMRPILTALVGSSIEPTWNPSITVDLDLNKEGRIPLSLDENRTQNIAEEIFREEVPRW
                     FKPGLAAAALAMVATFGAYLYLTAFDIPGTRQAAEKAAQDIVQGKRDIANLKKTRQEN
                     DILARRKALLTKLVGGVKHFSRSMEVVRDNTPKGIQLCEVTISDGELKLDGSAVDFAS
                     VGNLSVNLGSHPTVSQSNIEYALRPDKHPEVVAFSLKAQINSENRSENKTDKSPINST
                     PNHTALNPAGTSGGLTR"
BASE COUNT          754 a         1031 c         1192 g         1019 t
ORIGIN      
        1 tcactactca tagccgtgaa ctggcggatg ccatattgga taagagcggt tatgaccatc
       61 tggaactgaa gaaagaaaaa ggcgaaacca aactggttgg tgtcggtttg ggcggctaca
      121 gggaaaaaga tgaggagtag cggctccgca cctccagtag tagctcccgg gtgtaagccc
      181 agcgcctgaa gaagttatgc cagtagtcat ctgtctgacc ggtcgcgctt gtggcaacgg
      241 tttggttgga tgcaccggct gcgcttgctg cagtggccgt atcagccctt gtcatttcga
      301 agctgtctat ggctttgctc gctgctttca aggtggcgtc aaggtcttca ttactcaata
      361 gttgcgccag tcgacaattc cgctcgctca ccgcctgtga tgccagagct tgttcggtct
      421 tgccgctatt cttgtgctga atggaagcag ttgccgacat gattatgttt ttcacaatca
      481 aatcgtgact gacggccggg tgctcttgca cggcttgcct taaggctttg agaaagttgc
      541 cgctcaaatc tattggactg gtggaaaggg ctgccataaa accccgggcg gcaagatcgt
      601 tggccagcag ttccttcatg gtggcatgca tttcttcggt gctaactgca gtggtgttgc
      661 tgttttgcgc ctccgtccat ttctcgagta cacgcatgga gtctgagatg gactgttgaa
      721 tggtttgcat aattgtccct gtctagcgga ttgaaggcag atcaagctct tccatctgtc
      781 cgtgttcttc cattacggct ttctcgtctc tggctgagac cgagaccaga tgggcggtac
      841 cgaaactctc tcccggagcg agggtgattt gttcgggcca gtggttggcc cgcctgacca
      901 gcggatcttt cacttgaaaa atggccttat cgccgatgat gccgcttagt ctcaggtatc
      961 tcaatacgga aggcttttcc ggcggcatgg gcaggtcgga tagggcaaga ccactgggca
     1021 gtccgccgcc ggacatcccg cctggcaggg gtggcggcat atagccgtta tgtggtccgg
     1081 gcatggcgct catatcttgt tcgaggccgc tacccgtggg gggcggcggc accagttcga
     1141 gctggggaac gttcttgcct ggtattgcgg tcttaacgag cttgccgttc ttatctttca
     1201 ccgttggctg attctcggcg cctgccggta gacggggaaa ggactgcaat tgccctaagg
     1261 gctcaaaggg atctcttatt cccagggcgg cttttgcaga ttgccgtgcc gactcgatac
     1321 tggtgacggt ttccggacct ctcacctcac tttgcgcaag ctgctccggc ttgcggctga
     1381 cgatgccgtc ggcattggct actgccaggg ccggcaccag ttccgccggt gtggcctggg
     1441 ctctcagggc cgtgtccatg cccccgagaa cggcgggcag ccgattttta gctttcctga
     1501 ccgccgcagg caacggttgg gcgagcggat caggctctgt ctgctgaccg ccgaggttgt
     1561 tactccaggc ataggtagcg cctatgagaa tacagccaat tgccaatatg agaacgagac
     1621 gcattttgaa aaacactgtg gaaaattcgg actgccttgt atttatcatg ccccaattct
     1681 cagggaaggt agtaaatgtt gagcataaaa ctgagcaccg gttgacttaa tttcaacttt
     1741 tgcgcctttt ccccggcggc actcttattt tgaccgttgg gagaactggc cagggcgatg
     1801 gaaagggaat ttacggagag cactcgctgg taggcttcca tgcgtttcat gaagtctatc
     1861 aggctattgt aatcgccggt tatataaacc ctgcgactga gctgcttgac gcccatgttg
     1921 ttctgttcag ggcctttgtt ctcgtctttt gctttgttct gtccctgtcc ttgcacctga
     1981 ttggacaggt tttgcagagg agccggcagg tttaccttgc ggtctttcat cccggctgct
     2041 tccagcactt ccaggtcagc gccatcggcg ggagtctgat tattagcgcc cttctcgtct
     2101 tgtttttcca cactgattat gtcgactctt gaggccagcg ccatgcgttc caggtcgagc
     2161 aagagcaggt cgaggtaggg tgcgtggggg acttcgttgc gaagactgtt tatgtcttcc
     2221 tgcagcgctt tgtggctggc atcggcggag gctttgtcag tcagcttgcc tttcacttct
     2281 tgcaattcga tttcttgttt agccagctct tcatgttcgt gctgactatc taaaaatgcc
     2341 ggataggtca gggctagagc cccagtggcg gaaactgcca ggggaccaaa gacaatcagc
     2401 attctggtgc ggcggttcat ctggttagac ccccgcttgt gccggctgga ttgagagctg
     2461 tgtgattggg ggttgagttg atgggactct tatcggtttt gttctcgctt cggttttcgg
     2521 aattgatctg cgctttcagg ctgaaggcaa ctacttccgg gtgtttatcc gggcgcaggg
     2581 catattcgat attcgattga gaaaccgtag ggtggctgcc caggttgact gaaaggttgc
     2641 cgacgctggc aaagtccaca gccgagccgt ccaatttcaa ttcgccgtcg ctaatggtaa
     2701 cttcgcagag ctggatgcct tttggggtgt tgtctcttac cacttccatc gatctggaga
     2761 agtgtttgac gccccccacc agtttggtca acaaggcttt gcggcgggct agaatatcat
     2821 tttcttgtct ggtttttttc aagttggcaa tgtcgcgttt tccctgcacg atgtcctggg
     2881 cagccttttc tgccgcctgt ctggtgccgg gaatgtcaaa agccgtcagg tagaggtagg
     2941 cgccgaaagt tgccaccatg gccagtgcgg cggcggcaag acctggtttg aaccagcgcg
     3001 gtacttcttc gcgaaagatc tcttccgcta tgttctgggt gcggttttcg tccagagaga
     3061 ggggaatgcg accttctttg ttgaggtcaa gatcgacggt gatgctcggg ttccaggtcg
     3121 gctcaatgga ggagcccact agggccgtta ggatggggcg catgcgcgaa gagatgaagc
     3181 ggtcgtccac ggtcatatcc cgcatggggt cgccgagtat tgtcttcatg cgcagtttgc
     3241 tgctgaaata ttcctgcata ttgggcaaca tgcaaccggg gccggttagc agtacctggt
     3301 cgatttttac gtcgcccacc tgtgatttgt aaaactccag ggagcgcatt acttcgtcgc
     3361 cgatgtcact gaaaatggtg cgggccacct gtcccgcttg tcccatgcgg gcgtcggtgg
     3421 gcgtgatgtt gaagaccgga atttcaggca gcagttccat ggcctgatca aaggaaatgt
     3481 ccaggctgcg tgctaccgat tcggtcaggg cttccacccc caggatgatg gagcgaccga
     3541 aaaggggcat ggcgctgcgt acgatattga tgtcggtggc gtcctgcctg atgttaacca
     3601 ccatagaaag gtgtccggag gagcccagat aaccggccag agccagggcg cgaatcaccg
     3661 aaagggagga tacttccacc cgaccgagtt tgacgccggc ggcatcggcc atgcgccagt
     3721 aagactccac gatggaatgt tggatggcgg ccagaatcac atcgacgcgg cgcaccccgt
     3781 ccgggtctgt acgctcggtg gcattcatct ggcaccagtc cagattagcc tcgctgatgg
     3841 gaaagggaac gtggtttgtc gcttcctggg tgaccacatc ggccagttct tcgggcggca
     3901 tgccgaccgg tacgggcatc aggcggatca ctaccccttg ggccggcacg cagatattga
     3961 cggagggcga ctgcccgccg gtgggaacgc cgctct
//
LOCUS       DAOQNZ010000170         3996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10324_length_3996_cov_3.224309, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000170 DAOQNZ010000000
VERSION     DAOQNZ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10324_length_3996_cov_3.224309"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PKW73_04280"
     CDS             complement(<1..587)
                     /locus_tag="PKW73_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA72528.1"
                     /translation="MTRASHPYYQPGVIVSGNENLLELRDVAKTYGEHQVQALKHVNL
                     VVRKGSFACLMGQSGCGKSTLLNIIAGLDSPSSGSVVFDGIDMSALKDEEVTRIRGEK
                     IGFVFQFFNLLSTLTVEENVSLPLELTTNLSHKARRAKVLEMLERVGMQNRIDFFPAQ
                     LSGGEMQRVAIARALVHSPRLLVADEPTGNLDSANG"
     gene            953..>3996
                     /locus_tag="PKW73_04285"
     CDS             953..>3996
                     /locus_tag="PKW73_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNA72529.1"
                     /translation="MQTTEDDEPLLSHLSFRYPLRRYQTEILDLVDEKLKNGEREIHI
                     VAPPGAGKTIIGLQLIANFKRPALILAPNTTIQSQWGQKLDLFLPPDQVDFGCQDIIG
                     THEDKPLKPVTVMTYQVLSTPGKEQEYLTKLSHRSWVDELVKGRGLSIGEAELRILEL
                     LQNNPKAHQKEMSRHGSRLRKKLADIMDLNEVLHPNAVGLLQALRRQKFKLILFDECH
                     HLTDYWAAIMLHLIKYLDDPLVIGLTGTPPEKKSSSQEGRYVSLVGAIDYQVPTPALV
                     KEGGLAPFQDLVYFVEPTAKEFDFLEEQHEEFHKLLEELCGADWLMGMGRIPQAGQEV
                     KISEGRAMVVAQETTIPADLPPLTAYVIEQAVKIEKEIGFDKFLKESPALAQAYLRAL
                     WKLKLPRPRKIEFSEELTQSPVIDDWMIIIDDFAAHKLKLSSSKEHHDLFERIKQALR
                     KLGFGITEQGLRKQASPVDRVLAFSQAKSKAVTEILALEYRVLDDKLRACVVTDFERM
                     SATAVKNLEGVLNEESGGALAVMRTLLLSPIALYVNPCLVTGSLLLIDQRVAAQFQEA
                     AEKYLTENGYDFKLNVEEHAGGFSQVSANTGAWESRLYVAMATSIFERGITKCLIGTR
                     GLFGEGWDSQSLNTLIDLTTTTAPVSVKQLRGRSIRIQTEGAFGARKVANNWDVVCIA
                     PSLEKGLNDYQRFVRKHDGFFGICDDGQIECGVGHVHPSFSELTGSDVFASVDVFNRE
                     MMERALVRDKIYDHWKIGEPYNNRLVPCLEVSKLRNMALTPPHIRKNQKYKEHAGQMR
                     SALNGVYFEYLSLGSVAALVTLGMTFSLGALGFTAVLPVMAASILARLKANKLKDKFF
                     HEICRLPGPDQNPEQEENLNFRRRQGLTDIACAVLTALSQTKHLPPIKKESLQVTVRS
                     DGSYRVFLDDVEPRASKVFNQALKEVLAPIGNQPFLIPKYEFPLSEGQSEAESRRFFN
                     KYLSGRAEPRVAAYHAVPALLARSEKGREAFQESWNKYVSPG"
BASE COUNT         1017 a         1089 c         1033 g          857 t
ORIGIN      
        1 ccgttggcgg agtctagatt gccggtgggc tcatcggcca ccagcaggcg ggggctgtgc
       61 accagggctc gggctatggc caccctttgc atttctccac cggaaagctg ggccgggaag
      121 aaatcaattc tattttgcat gcccactcgc tcaagcatct ccagcacctt ggcccggcgc
      181 gctttgtgac tgagattggt ggtcaattca aggggcaggg acacattttc ttccacggtc
      241 aaagtggaaa gcagattgaa aaactgaaag acaaatccga ttttctctcc tctgatgcgg
      301 gtaacttcct catctttgag agcactcatg tcaataccgt cgaagaccac ggacccggaa
      361 gagggactgt ccagaccggc aatgatattg aggagggtgc ttttaccgca accactctgc
      421 cccattaagc aagcaaaact acccttgcgc accaccaggt tgacatgctt gagggcctgt
      481 acctgatgct caccgtatgt cttagcgaca tctctcagtt caagaagatt ctcgttgccg
      541 gacacgatga ccccgggttg gtagtaggga tgagaagcac gcgtcaagag tatagctgct
      601 aatgaaccgg gcaagaactg acgagcctgt aataaacctg acctagctta atttaaacag
      661 gggcttgtga acggaaccgg aacgcccgcc atattctgcc gtgttggacc tgaaaaaatc
      721 accttgcagt gaccctcgaa aacggtatga tctcactgtt attcctcaaa gaatgccaaa
      781 tgaatagaca aggcaccatc agcattgcca tgcatcacaa agctagaggg gcaatttata
      841 caaagtgaag aacgcaggaa cgaccccggg accctcaggc ttacttcaaa atttcgcttt
      901 atcggcaaag ccgaaggcag acactaactt taagcccgag gcaattagca acttgcaaac
      961 aacggaagac gacgaacccc tcctcagtca tctcagcttt cgttatcctc tcagacggta
     1021 tcagactgaa attcttgacc tggtggatga aaagctcaaa aacggagagc gggaaattca
     1081 catagttgct ccccccggag ccggtaagac catcatcggt ctgcagctta tcgccaattt
     1141 caagcgcccg gccttgattc tggcgcccaa tacgactatt caatctcaat ggggtcaaaa
     1201 gctcgatctc tttctgccgc ccgatcaagt cgacttcggc tgtcaggata tcatcggcac
     1261 ccatgaagac aagccactca agcctgtcac cgtcatgacc tatcaggtgc tctccacccc
     1321 gggcaaagag caggagtatc taacaaaact ctcacaccgc tcctgggtag acgaactggt
     1381 caaagggcgg gggctttcca taggggaagc agaactgaga attctggagt tgctgcagaa
     1441 caaccccaaa gcgcatcaga aggaaatgtc tcgccacgga agccgcctgc gcaagaagct
     1501 ggccgacatc atggacctaa atgaagtttt gcaccccaat gccgtaggtc tcttgcaagc
     1561 cctcaggcgg cagaaattca aactgattct ctttgacgaa tgtcatcacc tcactgacta
     1621 ctgggcagct atcatgctgc acctgatcaa atatcttgac gacccattag tcataggctt
     1681 gaccggcaca ccgcccgaga agaagagttc cagccaggag ggacggtacg tctctctggt
     1741 gggcgcaatc gactaccagg tgcccacccc ggccctggtc aaggaaggag gactggcgcc
     1801 ttttcaagat ctggtctatt ttgtcgagcc tacggcaaaa gaattcgact ttctggaaga
     1861 acagcatgaa gaattccata aattgctgga ggaactctgc ggcgccgatt ggcttatggg
     1921 catggggcgg attcctcaag ccggacaaga ggtcaagata agcgaagggc gggctatggt
     1981 ggttgcgcaa gaaaccacta ttccggcaga tcttccgccc ctgactgcat atgtcatcga
     2041 gcaggccgtc aaaatcgaga aagaaatagg tttcgataag tttctcaagg aatcgcccgc
     2101 cctggcccag gcttacctgc gcgccctgtg gaaattgaag ctgcccagac ctcgcaaaat
     2161 tgaattttcg gaagagttga cccagtctcc ggtcatcgac gactggatga ttatcatcga
     2221 cgacttcgcc gcccacaaac tcaagctcag ttccagtaaa gaacaccacg acctctttga
     2281 acgaatcaag caagccttgc gtaaactggg cttcggcatc accgaacagg gtctgcgcaa
     2341 gcaggcttcg ccggttgaca gagtgctggc tttttctcaa gccaagtcaa aagcagtgac
     2401 tgaaattctt gccctggaat acagggtgct cgatgacaaa ctgcgggctt gcgtggtaac
     2461 agactttgag cgtatgtcgg ctactgccgt caaaaatctg gaaggtgtgc taaacgaaga
     2521 gtcaggcggc gcactggccg tgatgcgcac cctgctttta agcccgattg cgctctatgt
     2581 caatccctgc ctcgtgacag gttctctgct cttgatcgat caaagagtgg cggcacagtt
     2641 tcaagaagca gccgaaaaat acctgacgga aaatggttat gatttcaaac taaatgttga
     2701 ggagcacgcg ggcggcttct cgcaagtgag tgccaatacc ggtgcctggg aatcgagact
     2761 ctatgtggct atggccacca gcattttcga gcgcggcatc acaaaatgct tgatcggcac
     2821 ccgcggtctc tttggtgaag gatgggacag ccagtctctc aatacgctca tcgatctgac
     2881 taccacaacc gcgccggtct ccgtcaagca attgcgcggc cgctccattc gtatccagac
     2941 ggaaggtgcc ttcggcgcca gaaaagtagc caacaattgg gacgtggtct gcatcgcacc
     3001 cagcctggaa aagggactca acgattacca gcgctttgtg cggaagcatg acggcttctt
     3061 cggcatctgc gacgacggcc aaattgaatg cggcgtcggg cacgtgcacc ccagtttttc
     3121 cgaattgacc ggtagcgatg tctttgcttc ggtggacgtc ttcaaccgcg aaatgatgga
     3181 acgggctctc gttcgggaca agatttatga ccactggaag ataggcgaac cttacaacaa
     3241 ccgactggtg ccctgcctgg aggtgtccaa gttgcgcaac atggcgctga caccacccca
     3301 catcagaaaa aatcagaaat acaaagagca tgccggacaa atgcgtagcg ccttaaacgg
     3361 ggtctacttc gagtaccttt cacttggctc ggtggctgcc cttgtcaccc tgggcatgac
     3421 ctttagcctg ggagctctgg gcttcactgc ggttctaccg gtgatggcag catcaattct
     3481 ggcgcgcttg aaagcaaaca agttgaaaga caagttcttc catgaaatct gtcgcctgcc
     3541 aggtcccgat cagaatcccg aacaggaaga gaatctcaat tttcgccgac gccaggggct
     3601 caccgacatt gcttgcgccg ttttgacggc cctttctcag acaaaacatt tgccgccgat
     3661 caagaaggaa agtctgcaag taacggtgcg cagcgacggc agctaccgag tctttctcga
     3721 tgacgtggag ccgcgcgcct ctaaggtttt caatcaggcc ctcaaggaag tactggcccc
     3781 tatcggcaat cagcccttct tgattcccaa gtatgaattc cccttgagcg aaggtcaaag
     3841 cgaagcggaa agtcggagat ttttcaacaa gtatctctcc ggccgagctg agccccgtgt
     3901 ggcggcctac catgcagtgc cggccttgct ggctcgctcg gaaaagggac gcgaagcctt
     3961 ccaggagagc tggaataaat atgtaagccc cggctt
//
LOCUS       DAOQNZ010000171         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10330_length_3994_cov_3.573496, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000171 DAOQNZ010000000
VERSION     DAOQNZ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10330_length_3994_cov_3.573496"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..566
                     /locus_tag="PKW73_04290"
     CDS             129..566
                     /locus_tag="PKW73_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA72530.1"
                     /translation="MGRNYEQACPVAKALELLGERWTLLIVRDLILGRSKFNELQESL
                     PGITASVLSERLKNLEKEGVVDSRLYSEHPPRLSYHLTKKGRDLGKIVGALYDWGQKY
                     TGVGNRLVGRKCGHSLEVDYFCPVCKSRAMPQNPLAAAGDYQD"
     gene            690..1193
                     /locus_tag="PKW73_04295"
     CDS             690..1193
                     /locus_tag="PKW73_04295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNA72531.1"
                     /translation="MSAHTNTETQPSQVREQLERSGKLSQGTNYSAAHLGALEQLTEY
                     PLFMPALNKTVKGKVFLKELIGTNGSEISLNKLPAGYTIPFSHAHKQNEEVYIFLKGQ
                     GQLAVDDDIVPVSEGSCVRLAPAALRCLRNSGQEELYFIVIQAREGSLGQYTFDDGIK
                     LEHNINW"
     gene            1156..2196
                     /locus_tag="PKW73_04300"
     CDS             1156..2196
                     /locus_tag="PKW73_04300"
                     /inference="COORDINATES: protein motif:HMM:NF021832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HNA72532.1"
                     /translation="MTESNSSTTSTGSNADERGAHPILAPMCRFVQTQTFVEIDEGAL
                     KYLASELAAAYGGPPVKVLHWLAAAPASVQVFINQAHLQEAVSFLTLFHAIGFCYWGE
                     PRWTFTSYGTTYNGSLALLHSLRENLYHLKNLAANQTTTPEQFSAGLGGVNRLCLLPE
                     RLEFFRDLTKRLQEGELTRLLQEEDCLTKAFYIEASYSGFQDTARIDTPQGKVILPFL
                     KRAQLLVSDIDYLIRLKGGSGLPEADRLTAFADYKVPQLLREAGALIYDPELSEKVDN
                     KIELPEDSLEELSIRASTIVAVDKLKRLIAQRGVSLSAAEVDNLLWLKSQKEAQAPSA
                     IRPYHRTMTCRY"
     gene            complement(2232..2828)
                     /locus_tag="PKW73_04305"
     CDS             complement(2232..2828)
                     /locus_tag="PKW73_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72533.1"
                     /translation="MSVPNGKTNCLSAKGRLCFQKLDECAKRNKGRDPFPAVMRDWQA
                     TKVYYLEFEQALSSVREESLSESEASGDKAGYLYLKASSVWQSLVETSSALSKVSLAS
                     KSNREAQVLEFERSMGNLDERIKDYQTKVRKLGLEDETTKRMASAFQVRAEGLRQVFR
                     ELNTERKDGGTRVLRLGSSELSSMLDSMRGRAMLDLQK"
     gene            complement(3104..>3994)
                     /locus_tag="PKW73_04310"
     CDS             complement(3104..>3994)
                     /locus_tag="PKW73_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72534.1"
                     /translation="LEGKLAQEPQDEHSRFTLIGYYCKRAIWKRVDRERVMNHELWFI
                     RNKPDHPELRFVVGSIVACWDSDLYELGKAAWLEHVHEGCTDVAILMNAASFFSMWDS
                     KMAIRLVERARVLEPSNRKLLHRLAHIHDLALLRNPDDTLLRRAYEVRKELAEAEPEA
                     IDQLVKFAEVALRCGDFSAARVAAQKVLEDKSSESQSIRSEAHSILGRIYLREGDLKA
                     ARVELLNCAANSEYELENEFVAIGEREIVCEHLWRSLPAWKFGRIQLLMWIFQLRLGG
                     SPTLKRTWLLSYGIGGYAGQ"
BASE COUNT         1072 a         1172 c          887 g          863 t
ORIGIN      
        1 cgcatctgct ctaccttgag gtatcatcat aagtaaatct gcgtttcaac cgacgaccga
       61 gaggtaagat atctggtcgc cgtagatata gcaagcatta cctgaagacc aataaggata
      121 taaggcggtt gggtaggaac tacgagcaag cttgtccggt agcaaaagcg ctggagctat
      181 tgggcgaaag atggacccta ctcatagtca gggacctgat tctaggacgc tctaaattca
      241 atgaattaca agaatcactg ccgggcataa ccgccagcgt gctttccgaa cgcctgaaga
      301 atctggagaa agagggcgtg gtcgactccc gcctctacag cgaacaccct cccagactct
      361 cctatcacct gaccaaaaag ggtcgcgacc tgggcaaaat agtgggcgcc ctctacgact
      421 ggggtcaaaa atacaccggc gtgggcaacc gactggtggg acgaaaatgc ggtcacagcc
      481 tcgaggtcga ctacttctgc cccgtctgca aaagccgggc tatgccccag aaccctctgg
      541 cggcggcagg cgactaccag gactgaacca ttcagcctcc caaccacttg aaaatattta
      601 ttgccaagtt acttgttttt tcctactcac taggtgttta atatctacag taaggttcgc
      661 aattgaaaca ctcataagag gtaaaacaaa tgagcgccca cacaaacacc gaaactcagc
      721 cctcccaagt gcgggaacag cttgagcgca gcggcaaact gtcccaggga acgaactaca
      781 gcgccgccca cctgggtgcc ctggagcaat tgaccgaata tcccctgttc atgcccgccc
      841 tcaacaaaac cgtcaaaggc aaggtcttcc tgaaagaact cataggcaca aacggcagcg
      901 aaatatcttt gaacaaactg ccggctggat ataccatccc cttcagccac gcccacaagc
      961 aaaacgaaga ggtctatatc ttcctaaaag gccagggtca actggccgta gatgacgaca
     1021 tcgtccccgt cagtgaaggc agttgcgtgc gcctggctcc cgccgccctg cgctgcctgc
     1081 gaaattccgg tcaagaagag ctatacttca tcgtcatcca ggccagggaa ggttcactcg
     1141 gtcaatatac attcgatgac ggaatcaaac tcgagcacaa catcaactgg tagcaacgcg
     1201 gatgagcgcg gcgctcatcc catattggca ccaatgtgcc gtttcgtgca gacacagact
     1261 ttcgtagaaa ttgatgaagg cgctctcaag tacctggcat ccgaactggc agccgcctac
     1321 ggcggtccac ccgtcaaggt tctacactgg ctggcggcag cgccggccag tgttcaggtc
     1381 ttcatcaacc aggcccatct gcaagaagcc gtgagcttcc tcaccctttt ccacgctatc
     1441 ggtttttgct actggggcga accacgctgg accttcacca gctacggcac cacatacaat
     1501 ggcagcctgg cccttctaca cagcctgcgc gaaaatctct accacctgaa aaacctggca
     1561 gcaaaccaga ctaccacccc cgagcaattt agtgccgggc tcggcggcgt caatcgttta
     1621 tgcctcttac ccgagcgact agaattcttc agagacctga caaagagact gcaagaaggc
     1681 gaactgactc ggctcttgca agaagaagac tgcctcacca aagcatttta catcgaagcc
     1741 tcttacagcg gctttcagga caccgcccgc atcgacacac cacaaggaaa ggtaatcttg
     1801 ccgttcctca agagggcaca acttctggta agcgacatag actatttgat cagactcaag
     1861 ggcggcagcg gtctacctga agcagatagg ctcacagcct ttgccgacta caaagtgccg
     1921 caactcttga gagaggcagg cgccctaatt tacgaccctg aactgagcga gaaagtagac
     1981 aacaaaatcg aactgccgga agacagcctg gaagagctga gcattagagc ctctacaatt
     2041 gtggcagtag acaaactcaa acgcctgatc gcccagcgcg gcgttagcct cagtgccgcc
     2101 gaagtggata atctactctg gctcaaatca caaaaagaag cccaagctcc aagcgccatt
     2161 cgaccttatc accgcacaat gacttgtagg tattgattgc ctggggcgga tggcaaacgc
     2221 agcagcgaga actacttctg caaatccaac attgctcgcc ctctcataga atccaacata
     2281 gacgaaagct cggaactgcc aagccgcaag acacgggtac caccatcctt tctttcggta
     2341 ttcagctccc tgaaaacctg cctcaatccc tcggcacgca cttgaaacgc ggaagccatg
     2401 cgcttcgtcg tctcatcttc cagccccaac tttctgactt ttgtttgata gtccttaatt
     2461 ctctcatcca aattccccat agaccgctcg aactccaata cctgcgcttc tctattagat
     2521 ttcgaagcca gactaacttt ggagagagct gacgaagttt ccaccagact ctgccagacg
     2581 gaactagcct taaggtataa gtagccggct ttgtctccac tagcctcact ctcagataaa
     2641 ctttcttctc tcacactgga gagagcttgt tcaaactcca aatagtaaac cttggtagct
     2701 tgccagtcac gcatgactgc aggaaagggg tcgcgaccct tgttcctctt tgcgcactca
     2761 tccaacttct gaaaacagag gcgccccttt gcagataggc agttcgtctt tccattcgga
     2821 acagacaatt tgctctccag agcctgtagc ctgcctagcg actgctgaat ctcagattca
     2881 ttgacttgcg ccccccttga agaaactacc ccgcgaagca tcaacaacaa atcatctcca
     2941 tataggaggt attgaactcg cttgcgatgc aaactaccat ctaatgagtt ctcgccacca
     3001 acactaaagg caaaacacaa agccaggagt agggagctac atgaggctaa agcaattttc
     3061 caacttctag aacccgagtt cacgcctttc tcctcctggc agcttattgc ccagcatagc
     3121 cgccgatacc gtacgaaaga agccaagttc gcttcaaagt tggactaccg cccaggcgca
     3181 gttgaaaaat ccacatcaat agctgaatcc gtccaaattt ccaggctggc aaactgcgcc
     3241 acaagtgctc gcaaacgatt tctctctctc cgatggctac aaactcgttc tctaattcgt
     3301 attcggaatt ggcggcacaa tttagcaact ctactctggc agccttcaaa tctccttctc
     3361 tcaaatagat tcttcccaga atggagtggg cttcacttcg gatggactga ctttcagatg
     3421 atttatcctc cagcaccttt tgggcagcaa ccctggctgc gctgaaatct ccgcaccgca
     3481 gggcaacttc cgcaaactta actagctggt ctatagcttc aggctcagcc tccgccaact
     3541 ccttacgcac ctcgtaggct cgacgaagca aggtatcatc aggattccgg agtaatgcaa
     3601 ggtcgtgaat atgagcaagc cggtgaagaa gcttgcggtt ggagggctct aaaactcttg
     3661 cccgctctac cagccgtatc gccatcttac tatcccacat ggagaaaaaa ctagcagcgt
     3721 tcataagaat agcaacatcg gtgcaaccct catgaacatg ttccaaccag gccgccttgc
     3781 ctaactcgta caggtcgctg tcccaacagg ctacaatact accgacaaca aagcgcagtt
     3841 ccggatgatc aggcttgttc cgaatgaacc acagttcgtg gttcattacc ctttctcggt
     3901 ccactctctt ccatatagca cgcttacagt aatatccaat caaggtgaaa cgcgagtgct
     3961 catcctgagg ctcttgagca agttttcctt caag
//
LOCUS       DAOQNZ010000172         3993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10333_length_3993_cov_3.948451, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000172 DAOQNZ010000000
VERSION     DAOQNZ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10333_length_3993_cov_3.948451"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(600..675)
                     /locus_tag="PKW73_04315"
     tRNA            complement(600..675)
                     /locus_tag="PKW73_04315"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:His,seq:gtg)
     gene            complement(753..2900)
                     /locus_tag="PKW73_04320"
     CDS             complement(753..2900)
                     /locus_tag="PKW73_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNA72535.1"
                     /translation="MASGKSDKESSTGTQVSSAGAAIEATRGGLAVQSSTSQENQVRN
                     IVLAGNPNVGKSVIFNALTGANADVSNYPGTTIDIAKGKLGKDDLADTPGVYGVSSFN
                     DEERTASKMILNADLVINVVSALTLERDLFLTLQLIEMGKPLLLVVNQSDEARARGLK
                     LDLAVLEATLGVKVIPCVAVENQGIDDIKHSLADATTGNACGKVLEILEDLAPYWKDE
                     HLLSQGEALLLAEGDQTVQESVKTRIPQALSHATASGARKAEIYSLRRHRVNELTERI
                     VERTRGTQMLSSKIGRTLLHPFWGTIISISVCYLIFYQMLGVWIAGNLVDLTEKKTMK
                     VYYEPFVRRLAASVFPTTITVNGKAYSFEEGTTREPQKARELDQAIKTVPADEVEYNF
                     WSKKNLMSVLGNIFVGDYGLATLTVTYLLGLLMPLVIGFYLGLSLMEDSGYLPRLAVL
                     VDRLMNKIGLNGRAIIPLILGLGCVTMATITTRLLTSTREKIIATALLGVAIPCSAQL
                     GVVSGTLARAGGAAAWAVYLIMVFSILAITGLLLNMVLPGKSTGLMIDLPPMRLPRLD
                     NVLRKTWKKSWNFLVDATPMFFLAGFAVSLAQMSGVLQLFMDALQPIVVNWLNLPADP
                     RIPTTFILGIVRRDFASFGLSDVPLTPVQAVTSMIVITLFVPCIATVGVMIKERGMKI
                     AMTIWLGSWLAAFAIGGLIARVLPFVFNPLGIR"
     gene            3195..>3993
                     /locus_tag="PKW73_04325"
     CDS             3195..>3993
                     /locus_tag="PKW73_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNA72536.1"
                     /translation="MRRVAISLMLVLLNSGALPGALAASIEEPTAASVAAEGKPGQVQ
                     EFTLANGLKVLLMEDHSFPVFSSMVFYRVGSRNENLGETGLSHLMEHLLFQRVGKLRK
                     GELGALIARNGGMFNGFTSDDFTVFFETMAPSKLELALRLESIRMQSATFTEDDLNAE
                     IKRIEKELELEAKDQVNLLVKEVKSAAFTRHPYKNPTIGWNTDVPKLTLDDVKRHYRE
                     YYQPGNATLVLVGDFQSRKAMELVRKYFEPLPKGQLPPAVRVVEPPQR"
BASE COUNT          935 a          988 c         1108 g          962 t
ORIGIN      
        1 cctactctat gggacgaatg gcgcctttgc tacgatgcca gttcccgaga cagtctttag
       61 ccttcccttt gtctcaaccg aagattggca agccgataga gagaagcaag ccagatatgt
      121 atttggcaac gctcttaagc aatggtttga tgtgctgctt tctcagattt atcgaactcc
      181 gatttggtgc cggtcagctg agtccaatga gtattccctg gttaagtcct tcagacttga
      241 tacgccttgg agcctaatgt gtatggagct tcataaaagc tatcttggca tgtcgaaata
      301 ttcctattgc gctacctgtc gtgagcctta ccttagaggc cctaaagcaa agggctgtag
      361 cgatgcatgt gcgcagaaac gatacagaga acgccgtaaa gcacggaagg agggcaacta
      421 gaaatgggcg tatacgaaga taaacaaagc ggcaaatacg gcattcgcat tgaatgtcat
      481 taccaaaata gtgtcttggt ttctaaagat gggtatgtct tcaccacgaa aagtggttag
      541 gccgatagac aaacatcctg accgcatctg ggctagattg agacaatagt aaaagaaaat
      601 ggggcagacg aggggactcg aacccccgca taccggaacc acaatccggg gccttaacca
      661 cttggcgacg cctgccatat gcgcaaaatt tatattaaca tgctcttccg gcagttcgtt
      721 tcaggggatg gctgtcccaa tattttgtaa ctttagcgaa ttccaagagg attgaagaca
      781 aagggcaaaa ccctggcaat gagaccgcca atggcgaatg cggccagcca ggagcccagc
      841 cagattgtca tggcaatctt catgccccgt tctttaatca tcacccctac tgtggcgatg
      901 cagggcacga agagggtgat gacaatcatt gatgtaacag cctgaaccgg cgtcagggga
      961 acgtccgata agccgaaaga ggcaaagtcg cggcgcacga taccgagaat aaaggtggtt
     1021 ggaatgcgcg ggtcggccgg caaattcagc cagttgacca caatcggctg caaggcatcc
     1081 ataaagagct gtaatactcc gctcatctgc gccaggctga cggcaaaacc tgccaggaag
     1141 aacatgggcg tggcgtcaac tagaaagttc caggatttct tccaggtttt tctcagcaca
     1201 ttatcaaggc gcggcaggcg catgggcggc agatcgatca tcagaccggt ggatttgccc
     1261 ggcagcacca tgttcaaaag tagccccgtg atagccagta ttgagaaaac catgatcaaa
     1321 tagaccgccc aggcggcggc gccgccggct ctggccaggg tgccggagac cacgccaagc
     1381 tgggcggagc atggaattgc gactcccaga agggccgtgg caatgatttt ttctcttgtg
     1441 ctggtcagca gtcgggttgt gattgtggcc atggtcacac agcccagacc taaaatgagt
     1501 ggaattatgg ctctaccgtt caagccgatt ttgttcatga gccggtctac cagaacagcc
     1561 agacgcggca ggtagcctga gtcttccatg agcgagagac ccaggtagaa gcctattacc
     1621 agaggcatca agagtcccag caggtaagtg actgtgagtg tggccagacc ataatcgcct
     1681 acgaagatat tgcccagaac cgacatcagg tttttcttag accagaagtt gtattccact
     1741 tcgtcggccg gcacggtttt gatggcctgg tccagttccc gggctttttg cggttccctg
     1801 gtggtgccct cttcgaagct gtaggctttg ccgttcacgg ttatggtggt aggaaaaacc
     1861 gaggctgcca gcctgcgaac aaagggttca tagtagacct tcatggtttt cttttccgtg
     1921 aggtctacta gattgccggc aatccacacg cccagcatct gatagaagat caggtagcaa
     1981 acagaaattg aaatgatggt gccccagaag gggtgcagaa gggtgcggcc gatcttggag
     2041 gagagcatct gtgtgccccg ggtgcgctcc acaatgcgct cggttagttc attgactcta
     2101 tggcgcctga ggctgtatat ctctgctttt ctggcaccgc tggcagtggc gtgagaaagg
     2161 gcttgcggta ttcgcgtttt gaccgattct tgtacggtct ggtcaccctc ggccagcaga
     2221 agcgcctctc cctggctgag gagatgctcg tctttccaat agggggcaag atcttcgaga
     2281 atctccagca ctttgccgca ggcattaccg gtagtggcat ccgcaaggct gtgtttaata
     2341 tcgtcgatgc cctggttctc aacggctacg cagggaataa ctttgacgcc gagggtagct
     2401 tctaggacgg ccaggtctag tttgagcccg cgggctctgg cttcgtcact ctggtttacc
     2461 accagaagca ggggtttgcc catttcaatc agttgcaggg tcaaaaacag atcccgctcc
     2521 agggtcaggg ctgagaccac atttatgacc agatcggcgt tgagaatcat ttttgaggcg
     2581 gtgcgttctt cgtcgttgaa gctgcttacg ccgtagacgc caggtgtgtc cgccagatca
     2641 tcctttccca gcttgccttt ggctatatcg atggtggtgc ctggatagtt ggaaacgtcg
     2701 gcattggctc cagtgagagc gttgaagatg actgatttgc ccacattggg attgccggcc
     2761 agcacgatat tgcgaacctg gttctcttga ctagtggagc tttgcaccgc cagtccgcct
     2821 cttgtggctt ctatggcggc gccggcgctg gaaacttgcg tgccagtgct gctttccttg
     2881 tcgctcttgc cggatgccat gaattgacgc cttgtgctcc aggttattga gaaattttat
     2941 caacagatgc atttcttcga tatgttcttg ctctcataac ctcatgagac ttaacggaag
     3001 tcctcaggct atggtacctt tcaccttggc cgggattgcg ggtgttccgc gttcgagttg
     3061 gtttgttgaa gcaatctgca gccacccgag cgatcctgaa acgcaaatta gaaaaaccaa
     3121 tcgagataga gccaaccttg aatagccagc aaatttttga agcctgccgg atgaaactta
     3181 aggcgccgtt cggtatgaga cgagtcgcta tcagcctgat gctggtgctg ctcaatagcg
     3241 gggctctgcc gggagccctg gcggcatcca ttgaagagcc gactgccgcc tctgttgccg
     3301 ccgaaggtaa gcccggtcaa gtacaggagt ttaccctggc gaacgggctc aaagttttac
     3361 tgatggaaga ccactccttt ccagtctttt cctccatggt tttctatcga gtcggttccc
     3421 gcaacgaaaa tctgggtgag acagggcttt ctcatcttat ggagcatctg ctctttcaac
     3481 gggtaggcaa attgcgcaag ggcgaactgg gtgcgttgat cgccagaaac ggcggcatgt
     3541 tcaacggttt taccagtgat gactttactg ttttcttcga gactatggcg ccctccaagc
     3601 ttgaattggc tttgcgcctg gagtcgattc gcatgcagtc ggccacgttt actgaagatg
     3661 atttgaacgc tgagatcaag cggattgaaa aagagcttga actggaagca aaggaccagg
     3721 tgaatctgct ggtgaaagag gtgaaatccg ccgctttcac caggcatccc tataagaatc
     3781 ccaccattgg ttggaatacg gatgtgccta agcttactct cgatgatgtg aaaaggcact
     3841 atcgggaata ctaccagcca ggtaatgcca ccctcgtttt ggtgggtgat tttcagtcgc
     3901 gcaaggctat ggagttggtg cgcaagtatt ttgagccctt gcccaaaggg cagttgccgc
     3961 ctgctgtgag ggtggtggag ccgccacaga ggg
//
LOCUS       DAOQNZ010000173         3980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10392_length_3980_cov_3.190318, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000173 DAOQNZ010000000
VERSION     DAOQNZ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10392_length_3980_cov_3.190318"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKW73_04330"
     CDS             complement(<1..186)
                     /locus_tag="PKW73_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72537.1"
                     /translation="MSKLKDLVDDKLQKFFAPPTFVVQDIVPADDVSKSIIEHQKYRY
                     QLASLTPLAVGPHGGRIL"
     gene            359..1759
                     /locus_tag="PKW73_04335"
     CDS             359..1759
                     /locus_tag="PKW73_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA72538.1"
                     /translation="MLPPRLLSKALALTLAISAFPTDYVKGSPGHGKPEPIKLLPQIP
                     SLDVLPQPKRLEGRVGESLILRPKVNALGYATDAPNPPETVTYVQPDSLAWKAGLANG
                     DKILSRAARKPYAALIVMRGGKKYLCMIDLRAAAPAQAPSAAAATKKTPAQILHERSL
                     VMVIDKSASMDTKDCPGGKSRWQWCKQHLEDIYRAENGLLQKEISIITFDSQPRSYAN
                     CSPAQLPSVFESTSPDGETNMAPALEDAFQIVRRSLEGGKPALLAIISDGRPTDAEAL
                     KKAIINRVNTLKQPKLLSIVFIEVGAPEKYLKELDTELVKQGAAQDVVSVVPLETAHK
                     DGWTNTLSRAVPQEDTISGGGAGVNKAKEALSAHIYTYDGKSQETTKGKDQVSQTQTV
                     KKQEPVKAHPTGTPAGTPVGTGSGAGQKPQTPAPVVKAHPTGTQAGSGAPAVAQPIEV
                     NEKETVLKRNANKVYK"
     gene            1756..2673
                     /locus_tag="PKW73_04340"
     CDS             1756..2673
                     /locus_tag="PKW73_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72539.1"
                     /translation="MKGQSIVKLTSLILALSCCRVSAQVPDTTKMLSLARKLDSQGRS
                     SDAVKLYDRILSQNMNSIPLLYESIEVKERAHRHGNLEDLNRIVASDPQAKKYPSVYN
                     FRAAALLSDNQEDAALKDCLTSRKLGRDTGLTSFVLCRIYQSRGEHEKAAHEMDFAMK
                     QYKLANLAYPHTLKALNLEALKKDKEALDEWNIACKLATKPEEGDLTNRARLCERLGK
                     YPQAIADYTSLITLNADNEFALEKRGRLYFKIGKYKECVADLSKSLKLDEDAAPSTAE
                     LLAAAYEKLGMKKEAAQELARAKKIRARM"
     gene            2780..2989
                     /locus_tag="PKW73_04345"
     CDS             2780..2989
                     /locus_tag="PKW73_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72540.1"
                     /translation="MDFWTVILILVAMSGLMGWFIVPFTSAVTWYCDWRDGNDINWGM
                     DTAFTIAVPFAWLFSIFEIMLLYTP"
     gene            complement(3005..3580)
                     /locus_tag="PKW73_04350"
     CDS             complement(3005..3580)
                     /locus_tag="PKW73_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA72541.1"
                     /translation="MPKIVDHDEYRRKIIDASFEIIAKRGYAAVSMRDLARTLRISTG
                     TLYYYFPNKLGLFEAILRKYGVDDMKKQAPDNINSPSFEDGVELLIEGMKRQEALWAK
                     LLLVQVDAYREPEGKLLSAFNTVFTSENTRDYLSNLLHIKNTDVLDTIRALMLGLIIQ
                     RVIGKRKVDWQVQKSVILETFAVHASVRSGG"
     gene            3786..>3980
                     /locus_tag="PKW73_04355"
     CDS             3786..>3980
                     /locus_tag="PKW73_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HNA72542.1"
                     /translation="MAQLDAFQMPGLVLEMYPEDHSPPHFHIIKDNWNIRIKFNLSII
                     KREIVWDSKYPESLKECPLTG"
BASE COUNT         1092 a         1104 c          959 g          825 t
ORIGIN      
        1 aagtatgcgt ccgccgtgag gacccacagc tagaggagta aggctggcta gctggtagcg
       61 atacttttgg tgctcaataa tagacttgga aacatcgtcg gcaggtacga tgtcctgcac
      121 cacgaaagta ggtggagcaa aaaacttctg caatttatcg tctacaagat ctttaagctt
      181 ggacatgact tgacttccct ccgggacacc cctgtgcaaa gagcaatagt aaccaaagga
      241 agcacaattt tgatcaaagt ttgccggaat gttggagcac gttgtcagtt ataatccgcg
      301 gcagctatca tgccggtaat tagcatggct gctattaccg agaatttaag gaaacaatat
      361 gctaccgccc cggcttctta gcaaagcact ggctctaacc ctggcaatca gcgcctttcc
      421 aaccgactat gtgaagggca gccccggcca tggcaagcca gaaccaatca agctcttgcc
      481 gcaaattccc agcctggatg tactacctca gcccaagcgc ctggagggaa gagtcggcga
      541 aagcctcatt ttgagaccca aagtgaacgc tcttggttac gctaccgacg cccccaatcc
      601 ccccgaaacg gtcacctacg tgcagcccga ttcactggcc tggaaggccg gtctggcaaa
      661 cggcgacaag atcctctcca gagcagcaag aaaaccctat gctgccctta tcgtcatgag
      721 aggcggcaag aagtatctct gtatgattga cttgagagcg gcggcaccgg cacaagcccc
      781 ttcagccgcc gctgcgacca agaaaacccc ggcccagatc ctgcacgagc gcagcctggt
      841 catggtcata gacaaatcgg cttccatgga tacaaaagat tgcccgggcg gcaaaagccg
      901 ctggcaatgg tgcaagcaac acctggagga catctaccgg gctgaaaacg gtctattgca
      961 aaaagaaatc agcatcatca ccttcgattc gcagccgcgt tcttatgcca actgctcccc
     1021 ggcacagtta ccgtccgtct tcgaaagcac ttcgcccgat ggtgaaacca atatggcacc
     1081 ggctctggaa gacgcttttc agatcgtgag gcgctccctg gaaggcggca agcccgccct
     1141 tcttgccatc atatctgatg gcagaccgac cgatgccgaa gccctcaaga aagcaatcat
     1201 aaatcgcgtc aataccttaa aacaaccgaa gttactttcc atcgtcttta tcgaagtagg
     1261 ggcgccggaa aagtatctca aagaattaga tacggaattg gtgaaacagg gtgcagccca
     1321 ggatgtagtc tcagtcgtgc ccctggaaac cgcccataaa gacggctgga ccaataccct
     1381 atcgagggct gtgccgcagg aagataccat ctccggtggc ggtgccgggg tcaacaaagc
     1441 gaaagaagca ctttcagccc atatttatac ttatgatgga aagtctcaag aaacaaccaa
     1501 aggaaaggac caggtaagtc aaactcaaac agtcaagaaa caggaaccgg taaaagccca
     1561 tcctacaggt actcctgccg gcactccagt cggcaccggc agcggtgccg gacaaaagcc
     1621 acagacgccg gcacctgtcg tcaaagctca tcctaccggt acacaggccg gaagcggcgc
     1681 tccggcggtt gctcaaccta ttgaggtaaa tgaaaaagag acggttctga agcgcaacgc
     1741 caacaaggtc tacaaatgaa aggtcaaagc atagtcaaat tgactagcct aatacttgcc
     1801 ctctcctgct gccgggtctc agcccaggtg ccagacacca ccaagatgct cagcctggca
     1861 cgcaaactcg atagccaggg gcgctccagc gacgccgtca aactctatga ccgcattctg
     1921 tcgcaaaata tgaacagcat cccccttctc tacgaaagca tcgaagttaa ggagagagcg
     1981 caccgacacg gcaatctgga agatctcaat cggattgtcg caagcgatcc gcaagctaag
     2041 aagtacccat ccgtatataa cttcagggcg gctgccctgc tcagcgacaa ccaggaagat
     2101 gccgccctga aagactgtct taccagtcgc aaactaggca gagacaccgg tctcacatct
     2161 tttgtgctct gtcgtatcta ccagagccgg ggcgaacacg aaaaggccgc ccatgagatg
     2221 gattttgcca tgaagcagta caaactcgcc aacttagcct acccccacac cttgaaggcg
     2281 ctcaacctgg aagccctgaa gaaagacaaa gaggccctgg acgaatggaa tatagcctgc
     2341 aaactggcca caaaaccgga agagggagat ctcaccaacc gggctcggct ctgtgagcgc
     2401 ctgggcaagt atccgcaggc catcgccgat tacaccagtc ttataacact caatgccgac
     2461 aatgaatttg ccctggaaaa gcgaggcagg ctctacttca aaattggtaa gtacaaagaa
     2521 tgcgtcgccg acctcagtaa atcgctcaag ctcgacgaag acgccgcccc ttccaccgcc
     2581 gagctattag ccgccgccta cgagaagctg ggcatgaaga aagaagcagc ccaggaacta
     2641 gctcgggcca agaaaatcag agcccgcatg taagaaattc cagcgcgaat ttaccttcgc
     2701 ctgcagtggg ggccttccca ttgccagatt tttttttgaa cactagcatc cgccggtcca
     2761 aaagctggag gtttgtttta tggatttctg gaccgttatt ttgattctgg tggctatgtc
     2821 cggtctaatg ggctggttca ttgtgccttt caccagcgcc gtcacctggt actgcgattg
     2881 gagagacggc aacgacataa actggggaat ggacacggcc ttcaccattg cagttccatt
     2941 tgcctggcta ttttcaattt tcgaaattat gcttctctat acgccctagc cgacaaaagc
     3001 ctcgctaacc ccctgagcgc acacttgcat gaacagcaaa agtctcaaga atgacagact
     3061 tttgtacctg ccaatcaacc tttcgtttgc caatcaccct ctgaatgata agccccagca
     3121 tcaaggccct gatagtgtcc aggacatcgg tgttcttgat gtgcagaaga ttggaaagat
     3181 agtctcgggt attctcggaa gtgaaaaccg tattgaaggc cgaaagcagt ttcccctcgg
     3241 gctctcggta ggcgtcaacc tgcacgagca agagcttagc ccagagggct tcctgccgct
     3301 tcattccttc aatgagaagt tccaccccgt cttcgaagga gggcgaattg atattatcgg
     3361 gggcttgctt cttcatatcg tcgacgccgt acttgcgcag tatggcttcg aaaagtccca
     3421 acttgttggg gaagtaatag tagagcgtac ctgtagaaat tctcaaagtg cgagccagat
     3481 cacgcatgct cacagcggcg tagccccttt tggcaattat ctcgaagctg gcgtctatta
     3541 ttttgcgcct gtattcatca tgatcaacaa tcttaggcat ggcaaagatt ataacaaaac
     3601 actcgttata aatagatgcg cgggtagggg aacagtcggc gcccaagacc caaaagtttc
     3661 tgacactcga aacaagctca atccgacctg gcgccccaaa tcggacatct acctcacccc
     3721 cttgcgcaaa tgtccgattt tagtataata cttgatagtc gggcgtgaat ctagcaggaa
     3781 cttcaatggc acagcttgac gcatttcaaa tgcccggctt agtcttagag atgtatccgg
     3841 aagaccactc cccacctcac tttcacataa tcaaggacaa ttggaacata cgcatcaagt
     3901 tcaacctcag cattatcaaa cgtgaaatcg tatgggactc taaataccca gagagcctga
     3961 aagagtgccc gttgacaggc
//
LOCUS       DAOQNZ010000174         3976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10410_length_3976_cov_3.139505, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000174 DAOQNZ010000000
VERSION     DAOQNZ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10410_length_3976_cov_3.139505"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKW73_04360"
     CDS             <1..356
                     /locus_tag="PKW73_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72543.1"
                     /translation="GTRCASICGGFIALYDALSKLRKSGVFDDLPITEHMAAVSVVQI
                     NGQLLIDPNYSEDSQAEMDANIVLTESGKILELQMTSEAKPYDPTKLADLMSLANKGI
                     KEIISLQTETLNKRS"
     gene            537..2084
                     /locus_tag="PKW73_04365"
     CDS             537..2084
                     /locus_tag="PKW73_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNA72544.1"
                     /translation="MDENLDFNPIRESFRDVTAWKLFMSTVAVMTRIYRLQGFKAAYR
                     VLPIVWTIIWSTRGFSRYHVEEFERAKESMGEAVDKEEAALSSEDYEQYRLLGRWLTR
                     RLHDLGPTFIKIGQTLSTRADLLPLPAMLELAKLQENVDPFPYEIAQEVIERELGASP
                     ETLYKEFDKVAIAAASLSQAYKATLFDGREVVVKVQRPDLTKIIVVDIQIIAAIADEV
                     MKYPSLCRHTDWPGVVEEFARTILEEVDYIREGRNADTFRSNYRNLDRIYIPRIIWKL
                     TGRRVLTIEYVSGLRITDVEGIKAMGLDPEEITRTGANFYLRQLLEDGFFHADPHPGN
                     MRVMADGRVGIFDFGMVGRLSPELKQHLVNAFLHTVQREYRLLVDDFVGMGFLNADAD
                     RDALCRDLTPIIDARFADGLTRVRFRKMLFDFSEVCFDYPFRLPSEFTYVMRALLTLE
                     GIALTINPKFNFVDAAMPFAHRLVMKNNQVLSQTIFKEVFNDGKFNRQAAIKLIKTAA
                     ALTGSLR"
     gene            2300..2935
                     /locus_tag="PKW73_04370"
     CDS             2300..2935
                     /locus_tag="PKW73_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNA72545.1"
                     /translation="MLTGELTNSNFASQPLNSITPQVVGCSRCRSRIETTAKFCGNCG
                     APQAQKTLPPGSGRSLAEDLSQVSLKLSESDIEASNRLNKEQAQKAKAQAMPSFARKR
                     SRRIAPELREEMNTIYMMMIREKFFLAMHCVIFLVINLIGFFIAFKCYFEYIGDEVTK
                     IMVATTPLLFINLVALTCLVPIKGTKRELARLKERETYIKLRLEYDSVMEV"
     gene            complement(2996..>3976)
                     /gene="gcvPB"
                     /locus_tag="PKW73_04375"
     CDS             complement(2996..>3976)
                     /gene="gcvPB"
                     /locus_tag="PKW73_04375"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNA72546.1"
                     /translation="EILVPDAAHGTNPATAIMCGFTVREIPTDAQGDVDLAALREAVG
                     PQTAGLMLTNPSTLGVFERNISEIAAIVHAAGGLLYYDGANLNAILGKARPGDMGFDV
                     CHLNLHKTFSTPHGGGGPGGCAVACRDLLEPFLPKPVIKKETNGFKWDFDRPQSIGKV
                     KGFYGNFGILVRAYAYILAHGGNGLHQVSKDAILNANYLKVKLSRNFVVAHSQPCMHE
                     FVLSGVKQKERGVATLNLAKRLLDYGVHAPTVYFPLVVPEAMMIEPTETENKETLDRF
                     AEIMVTIDKEIDENKELVMSAPLKTPVGRLDEATAARKPNLRWTPNPQAV"
BASE COUNT          965 a         1127 c         1015 g          869 t
ORIGIN      
        1 ccggcacccg ctgtgccagt atctgcggcg gcttcatcgc cctctacgat gccctttcca
       61 agttgagaaa gtcgggcgtc ttcgatgatc tgcccataac tgagcacatg gcagcggtat
      121 ccgtggtgca aatcaacggt cagcttttga tcgaccccaa ctactcggaa gattctcagg
      181 ctgaaatgga tgccaacata gtcctcaccg agtccggcaa aatcctggaa ttgcaaatga
      241 cctcggaagc caagccttat gaccccacaa aactggcgga tctcatgagc ctggccaata
      301 agggcataaa ggaaatcatc tctctgcaaa ccgagaccct gaacaagcgc tcctgaaaaa
      361 cgggcaaacc aaaagccaag gtcaggtaat gatttaagtt ctgtcactag aaacttaaat
      421 ttatgctttt tcctcgtgac aacatcaaat aagtctatac acttataggg tccggtgaat
      481 tttcgcagcc gccaaaattc acccctggcg caagagtggc atagacggaa gtgacgttgg
      541 acgagaatct agatttcaat cccatcaggg aatcattccg ggacgtcacc gcctggaaac
      601 tcttcatgtc caccgtggcg gtcatgaccc gcatatatag actgcaaggg ttcaaggctg
      661 cttaccgtgt gctgcccata gtctggacaa tcatctggtc tacccgaggc ttttctcgct
      721 accacgtgga agagttcgaa cgggccaagg aatctatggg agaggccgtc gacaaggaag
      781 aagccgccct atcgagcgaa gattatgaac aataccggct tttgggtcgc tggctgaccc
      841 gaagactgca cgatctgggc ccgaccttca tcaagattgg acagaccctc tccacccggg
      901 ccgacctttt gcccctgccg gccatgcttg aactggccaa actgcaggag aacgtcgatc
      961 ccttccccta tgaaatcgcc caggaagtca tagaaagaga actgggcgcc agtcccgaga
     1021 ccctctacaa agaattcgac aaggttgcca tcgctgcagc cagtctctct caggcttaca
     1081 aagctactct cttcgacggc agggaagtag tagttaaggt gcaaagaccg gacctgacca
     1141 aaatcatcgt ggtggatatc cagatcatcg ccgccattgc cgatgaagta atgaagtacc
     1201 ccagcctctg ccgacacacg gattggccag gagtagtaga ggaatttgcc cgcactatcc
     1261 tggaagaagt cgactacatc agagaaggcc gcaacgccga caccttcaga agcaactacc
     1321 gcaatctgga tcgtatctac attccccgca tcatatggaa gctgaccgga cggcgcgttc
     1381 tcaccatcga gtatgtttcc ggtctgcgta ttaccgatgt ggaaggcatt aaagccatgg
     1441 gtcttgatcc cgaggaaatc acccgcaccg gcgccaactt ttacttgaga cagctcctgg
     1501 aagacggctt cttccacgcc gacccgcatc cgggcaatat gcgcgtcatg gccgacggcc
     1561 gggtgggcat cttcgacttc ggcatggtgg gcagactctc tccggaactg aaacaacacc
     1621 tggtcaacgc cttcctgcac acggtgcaga gagaataccg ccttctggtg gatgactttg
     1681 tcggtatggg cttcctcaat gctgatgccg accgggacgc gctctgccgc gatctcaccc
     1741 ccataatcga tgctcgcttc gccgacgggc ttacccgcgt gcgttttcgc aaaatgcttt
     1801 tcgacttctc agaagtctgc ttcgactatc ccttccggct gccttccgag ttcacttatg
     1861 ttatgcgcgc cctcctcacc ctggaaggca ttgcactcac catcaatccc aagttcaact
     1921 tcgtggatgc ggccatgccc ttcgctcatc gtctggtcat gaagaacaac caggttttga
     1981 gccagaccat cttcaaagaa gtcttcaacg acggcaagtt caatcgtcag gccgccatca
     2041 agttgatcaa gacggcagca gctctgaccg gctctctcag atagccactt tacaattcag
     2101 gcatttcaag ggcgtttttg cccggtttat cccactaaaa gccccggaaa cgcttaaaag
     2161 tttctagggt gccccaaatt tgggcatcca ggctgcaaaa tatcaatgca aagaggtacg
     2221 gagctgctac tgcaccaaaa cagccaggca gttctaacga acaaactttc cgacaagctc
     2281 acccctaaag gtacgtgcct tgcttaccgg cgaactgacc aattccaatt ttgccagcca
     2341 acccctgaat tcaatcaccc cccaagtggt gggatgcagc cgttgccgca gcagaataga
     2401 gactaccgct aaattttgcg gcaattgtgg cgccccgcaa gctcaaaaaa ctctgcctcc
     2461 cggctcgggg agaagtcttg cggaagacct ctcgcaggta tctctcaagc tctcagaatc
     2521 ggatatcgaa gcctctaaca gactgaacaa agaacaggcg cagaaagcga aagcgcaggc
     2581 catgcccagt ttcgcccgca agagaagccg ccgtattgcc ccggagttga gggaagagat
     2641 gaacaccatc tacatgatga tgattcgcga aaaattcttc ctggccatgc actgcgtcat
     2701 ttttctggtt attaacctga ttggcttctt cattgccttc aaatgctatt tcgagtacat
     2761 cggcgatgaa gtgacaaaga taatggtagc caccactccc cttctcttta tcaacctggt
     2821 ggcactcacc tgcctggttc ccatcaaggg caccaagagg gaactggcaa ggctcaaaga
     2881 acgcgaaacc tatatcaagc taagactcga gtacgacagc gtcatggaag tctaaaaaga
     2941 aagagagcgg cacttttcag tccgctctct cctttttatt tgtccaagaa cgctcttaga
     3001 ctgcctgcgg gttcggagtc cagcgcaaat taggtttacg ggcagccgtc gcttcatcaa
     3061 gacgaccgac cggtgtcttg agaggagcgc tcatgaccag ctctttattc tcatcgattt
     3121 ccttatcgat ggtgaccatg atctcggcaa agcgatcgag ggtttctttg ttctcggtct
     3181 cggtaggctc aatcatcata gcctcgggta ctaccagcgg gaagtaaacc gtgggggcat
     3241 gtacgccgta atcgagcaga cgtttggcca gattgagagt ggcaacccct ctttccttct
     3301 gcttcacccc ggagagaacg aattcatgca tgcagggctg gctgtgggcc accacaaagt
     3361 ttctggagag tttgaccttg aggtagttgg cgttcaagat ggcatctttg gaaacctgat
     3421 ggagaccatt gccgccgtgg gccaggatgt aagcgtaggc ccgcactaga atgccgaagt
     3481 taccatagaa gccttttact tttccgatgg actgaggacg gtcgaaatcc cacttgaagc
     3541 cgtttgtttc ctttttgatg accggcttgg gcaggaaggg ttccaaaagg tcacggcagg
     3601 ccacggcaca accgcccggt ccaccgccgc catggggagt ggaaaaagtc ttgtgcagat
     3661 tcaaatggca gacatcgaag cccatatcgc cgggacgggc cttgccgagg atggcgttga
     3721 gattggcgcc gtcgtagtag agcaggccgc cggcagcgtg aacgatcgcg gcgatctcgc
     3781 tgatgttgcg ctcgaacacg cccagggtgg agggattggt cagcatcagc ccagcggtct
     3841 gcggcccgac cgcctcgcgc agcgcggcta gatcaacatc gccctgggca tcggtgggga
     3901 tttcccgcac ggtgaagcca cacatgatcg ccgtggccgg gttggtgccg tgtgccgcgt
     3961 ccggcaccag gatctc
//
LOCUS       DAOQNZ010000175         3957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10497_length_3957_cov_3.897745, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000175 DAOQNZ010000000
VERSION     DAOQNZ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10497_length_3957_cov_3.897745"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKW73_04380"
     CDS             complement(<1..212)
                     /locus_tag="PKW73_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72547.1"
                     /translation="MLRSLRIFRRVCIETLLGLRASGWSNWLVVSILAVALTIFGGVL
                     QMTMTLKNLVNAWGNQIQISAYLPDSA"
     gene            complement(216..902)
                     /gene="ftsE"
                     /locus_tag="PKW73_04385"
     CDS             complement(216..902)
                     /gene="ftsE"
                     /locus_tag="PKW73_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNA72548.1"
                     /translation="MIRLQNVCKNYGGSPALSNINLHIGLGEFAFLVGPSGAGKSTLM
                     RLLYREEVPTSGNVLISGVNLNRLSKGQIPLLRRRLGIIFQDFKLLNSQTVFNNVAYV
                     LRALGVDEREVNKRVSSALAVVGLEHKRDSYPTELSGGEQQRVGIARAIVNGPPVLLA
                     DEPTGNLDPATSLEIVQLLERISQRGTTVLISTHDQPIVNAMRRRVIALKGGELVADV
                     DNGFYELEMA"
     gene            complement(1084..1821)
                     /locus_tag="PKW73_04390"
     CDS             complement(1084..1821)
                     /locus_tag="PKW73_04390"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNA72549.1"
                     /translation="MDKPPFNKKPNGKEGPSSKEAAPPRPAFKRSKANLLQLSSVLPK
                     VVRALGIDRRLKEHTFMNLWPHVVPEPFASRARPLFIDAEGNLVVAVKDAAVGQEFSF
                     ARQEMLKRLQTAARGVGMNISGLRFDMKRFYEGKLEAAQEAMAAVRLPDPSAEDLAAV
                     VLTEEELEPTRYLGENVIDDAALVQRMRGLYEKELRLKRWREANGYPHCNYCGEVTSR
                     LHGTDLICALCFASGMSNKEPRGSGSI"
     gene            1950..2282
                     /locus_tag="PKW73_04395"
     CDS             1950..2282
                     /locus_tag="PKW73_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNA72550.1"
                     /translation="MLAQETIINELKKRFKPEAARDITANYLINIKGAGGGAWLTKID
                     SGTLSFEPYQDGLSPAYDCKVSIDAEDLAMIIDGKMSAMTAALSGVLSIEGELGMAMK
                     LVPVFFEG"
     gene            complement(2283..3182)
                     /locus_tag="PKW73_04400"
     CDS             complement(2283..3182)
                     /locus_tag="PKW73_04400"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNA72551.1"
                     /translation="MTFLRLGLIGNPLGHSLSPQIHGAFLRQFSLPGRYDLRAGANAA
                     SLLDQAIEDGYTGLNVTIPHKVAVIKSLPALSEEARLVGAVNTIRLKWDEDADDTECG
                     IMGFNTDIAGLSRTLRQAPAKFPGQKAILLGGGGAARACLVAMQRLSMSGCHILCRNP
                     QNVFSMLKELALSGFDPVFLQEINVLTIDELPGKLDQLADAKLFVNATPLGQTKDELP
                     FWLTPLLSALIRDAFIFDLVYSAGGLTPVITLAAKLGFALHCDGREMLVEQARQAFKI
                     WTGLTPSYQVGLEALEKALKSRV"
     gene            complement(3194..3622)
                     /locus_tag="PKW73_04405"
     CDS             complement(3194..3622)
                     /locus_tag="PKW73_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNA72552.1"
                     /translation="MSMGAVGDQFTDINVTPLTDVFLVLLVIMILIAPLIDKSDLKIK
                     PPETKNAKKDEATKGISIDIDKDGQLAINGKYVRDHNVDTIKAMILELKNAAQPGTDL
                     HLTLNADGDAKQKDVVEVMSAAAAAGITKMRVATQQQTNY"
     gene            complement(3660..>3957)
                     /locus_tag="PKW73_04410"
     CDS             complement(3660..>3957)
                     /locus_tag="PKW73_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNA72553.1"
                     /translation="AVLGTIGATCPFLGLFGTVVGVVFTLDQLGAQGGGTPVVVTGVA
                     KALIATGYGLIVAILAVVFNNTYTNTVKRFNDDFLLLKLLFLSFVGGESTAARG"
BASE COUNT         1015 a         1063 c         1028 g          851 t
ORIGIN      
        1 gcactatcag gcaggtaagc agatatttga atttgattgc cccaggcatt aactagattt
       61 ttcaaggtca tggtcatctg caagacgcca ccaaatatgg tgagagccac ggccaggata
      121 ctgactacca gccagttgga ccaaccacta gctctcaggc cgagcaaggt ctcaatacaa
      181 actctgcgaa atattctcag ggaacgcagc ataagctatg ccatctccaa ctcgtagaaa
      241 ccattgtcca catcggcgac cagctcgccc cctttcaggg caatcacccg tctgcgcatg
      301 gcattgacga tgggttgatc gtgggtggaa atcaaaactg tagtgccgcg ctggctgatg
      361 cgctcgagca attgcacaat ctcaagagat gtggccgggt cgaggttacc ggtgggctca
      421 tcggcaagca agaccggcgg accattgact atggcccggg cgatacccac tcgctgttgt
      481 tcaccacccg ataattcggt ggggtaggag tcgcgcttgt gctccaaacc gacaacggcc
      541 agggcactgg agacacgctt atttacttct ctttcgtcaa caccaagggc tctcaacaca
      601 taggccacgt tgttgaaaac ggtctgactg ttgagcagtt tgaaatcctg gaaaatgatg
      661 ccgagacggc gtcggagcag gggaatctgc cctttggaaa gacggttcaa gttgacgcct
      721 gatatgagca cattgcccga tgtcggcact tcctcacgat aaagcaggcg catcaaagta
      781 gatttgccgg caccgcttgg accaaccaga aaggcgaact cgccaaggcc tatgtgcaaa
      841 ttgatattgg atagggcggg gctaccgccg taatttttgc aaacgttttg cagtcgaatc
      901 atatcgaagt gctttacctg gtctccggga gcctcatgat aacacagtag tcacgaggta
      961 aatccagggt atgagaaagc ctagtataga ttaaggaaac cggctcagga gcaccttaac
     1021 agcgagcaga ccctggttga aagagggacg gtgagcctac caccggcctg ccccatatat
     1081 gagctaaata cttccacttc cccttggttc cttattggac ataccggaag caaagcaaag
     1141 ggcgcatatt agatctgtcc catgtaagcg gctcgtcacc tcaccgcagt aattgcagtg
     1201 gggatagcca ttagcctcac gccagcgctt caatctgagt tccttctcat agagcccacg
     1261 cattcgctgc acaagagcgg catcgtctat gacgttttca cccagatacc gggtcggctc
     1321 cagttcctcc tcggtcagta ctacggcagc cagatcctcg gcacttggat caggcaaacg
     1381 cacggccgcc atagcctcct gagccgcctc taatttccct tcatagaagc gcttcatgtc
     1441 aaaacgcaaa ccggaaatat tcataccaac gccgcgggca gcagtctgca gccgtttcag
     1501 catctcctgc ctggcaaagc tgaactcctg accgacggcg gcatctttca cggctaccac
     1561 cagattgccc tcagcgtcta tgaaaagtgg tctggctctg gaagcaaacg gttccggcac
     1621 cacgtggggc cagaggttca taaaagtatg ctctttcaag cgccggtcta tacccagcgc
     1681 ccgcacgacc ttgggcagta cggaagagag ttgcagcagg ttggctttgc tgcgcttgaa
     1741 ggctggacga ggcggcgccg cctcctttga ggagggacct tctttgccat tgggcttctt
     1801 attaaacggc ggcttatcca aaataccgaa ctccctgagc accccaggct atcacaaaaa
     1861 tttcctcata taagccgacc tggagtttca ggctatgcac gaaggttata atcaaaaccg
     1921 atactaaaca taattattag gtacagactg tgctcgcgca agaaacgata atcaacgaac
     1981 tgaaaaaacg cttcaaacct gaagcggcca gagacatcac cgccaattat ttgatcaata
     2041 tcaaaggtgc aggcggcggc gcctggttga ccaaaatcga tagcggcact ctcagctttg
     2101 aaccctacca ggatggtttg tcaccggctt atgactgcaa ggtgtcgatt gatgccgaag
     2161 atctggccat gatcatcgac ggcaaaatgt cggccatgac cgctgccctc tcgggagtgc
     2221 tttccatcga aggcgaactg ggcatggcta tgaagctggt gccggtgttt ttcgaaggct
     2281 aactaaacgc ggctcttaag agctttctcc agagcctcca aaccaacctg gtaggaagga
     2341 gtcaagccgg tccagatctt aaaggcttga cgggcttgct caaccagcat ttcacgccca
     2401 tcacagtgca gggcaaaacc cagtttggca gccagtgtta tcaccggtgt caaaccgccg
     2461 gcactgtaga ccagatcgaa aataaaggca tcacgaataa gggccgaaag cagaggcgta
     2521 agccagaacg gcagctcatc tttagtttgc cctaagggcg tagcattgac gaaaagtttg
     2581 gcatcggcaa gttgatccag cttgcctggt aattcgtcga tggtgagcac atttatttct
     2641 tgcaaaaaga caggatcaaa gcctgataga gccagctctt tcagcatgct gaagacattc
     2701 tgcgggttgc gacagagaat atggcagccg ctcatagata acctctgcat ggctaccaga
     2761 caggctctgg ccgcaccacc cccgcccagg aggatagcct tttgaccggg aaacttagcg
     2821 ggagcctggc gcagggtgcg gctcagaccg gcgatgtcgg tattgaaacc cataataccg
     2881 cattcagtat catcagcatc ctcatcccac ttgagccgaa tagtattcac agccccaacc
     2941 agtctggctt cctccgaaag ggccggcaga gacttgatca ccgctacctt atggggaatg
     3001 gtgacgttca gaccggtata accgtcctca atagcttgat cgagaaggga agcggcattg
     3061 gcaccggcgc gcaggtcata tctgccgggc aaggaaaact gacgcaaaaa cgccccatga
     3121 atctggggag aaagggaatg cccaagaggg tttccaatta gaccaaggcg aaggaaagtc
     3181 acagccgagc tagctagtag ttggtctgct gctgagtggc aacacgcatt ttggtgatac
     3241 cggcggcggc ggcggcagac attacttcca ccacgtcttt ttgcttggca tcaccatcgg
     3301 cgttgagagt caggtgcagg tcggtaccag gctgggcggc gttcttcaat tcgagaatca
     3361 tagccttgat ggtatcgaca ttgtgatcgc gcacatattt gccgttgatg gccaactgcc
     3421 cgtctttatc tatatcgata ctgataccct tggtggcttc gtctttcttg gcgttcttgg
     3481 tctcgggcgg cttgatcttc aaatcggatt tgtcgatcag gggagcaatc aagatcatga
     3541 ttacgagcaa gacaaggaaa acgtctgtca aaggagtgac gttgatatca gtaaactggt
     3601 cgccgaccgc tcccatcgac atggcgcaaa acctcctggt ggctgcttct tgctaaagac
     3661 taacctctag cggctgtaga ctcaccacca acgaagctga ggaacaacag cttgagcaag
     3721 aggaagtcat cgttgaaacg cttcacagta ttagtgtaag tgttgttgaa gacaactgcc
     3781 aggatagcca cgatcagacc gtaacctgta gcaataagag ctttagccac accggtcaca
     3841 accaccggtg taccaccgcc ctgggcgccg agctggtcaa gggtgaacac aaccccgacc
     3901 acggttccga aaagaccaag gaagggacag gtggcaccaa tggtacccag taccgcc
//
LOCUS       DAOQNZ010000176         3938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10585_length_3938_cov_2.799639, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000176 DAOQNZ010000000
VERSION     DAOQNZ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10585_length_3938_cov_2.799639"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKW73_04415"
     CDS             complement(<1..614)
                     /locus_tag="PKW73_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72554.1"
                     /translation="MLERNRRLSSMMLAITVVSAGTLTTSLQAQAAEKKGAKSKPAEK
                     AAPKGKTNPEAFALLKEGIRLSGEGKGQEAEAKFNEAIKLHPGYARAYANRSNIHMMR
                     KHYRRAIADCDKALSLDDKLGMAYINRSWAELALGQVQKALDDASKGIELEPKMAIAY
                     VNRSHAYNRLGKTKEAMADANKALELSPNLAVALVNRAHTYVLEG"
     gene            complement(669..2252)
                     /locus_tag="PKW73_04420"
     CDS             complement(669..2252)
                     /locus_tag="PKW73_04420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA72555.1"
                     /translation="MTGKVSNLEDGKIAGYPSKSREQSRLCRPQTAGGKFTLVTLAIC
                     AGSLLLCGCNSKQETSGGEKTGEQTSSTGSGTTTEPAKSEDKTGQASQTNTPDDATAK
                     EPAVTGGNVKLPNAVNLATLPPTMVICTVDGTPVTISQFKREYREAIGSLQAVLGMQP
                     MQVQELVKQATAAGVSLTAEEKKKLLEAARKPQSLEGKSLDKFLKEKKLTEAEFDKQV
                     IDLGLAFKVGAKLIEQQLLTELINRELLLAEAKAQNFYKPAYNNYLKVKDLPKYKKML
                     EMSDQTPEEIKEDLIQGEMLIMLMQKIARESVASDKELLAFYQKNKEAFKHGEKVRLS
                     HIVLAAPRVDAPPMESIKTQLKKQNPTMSDADLDKEVLVVKQQKMRLAQELLARANKG
                     EDFKSLANDNTEDMQARQAKNGGDLGYIDVVAAQAGPDQKALLDAVKYLKPGQLALTP
                     VETNFGYHVIKLTEKVPAGTFSFDEIKDQLKEKFVAQNMEAAKTRWLVNKRKSAKIVF
                     SDEFRKASSTASVPQANVK"
     gene            complement(2263..>3938)
                     /locus_tag="PKW73_04425"
     CDS             complement(2263..>3938)
                     /locus_tag="PKW73_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNA72556.1"
                     /translation="EETPDQWQAIQDVKNDLESSKPMDRLICGDVGFGKTEIAIRGVF
                     KTVMSGKQVAVLVPTTILAQQHYNNMADRFAPYPVKVGLLSRFRTAKEQREVAKRLTS
                     GECDVVIGTHRMLQKDIAFKDLGLVVIDEEQRFGVAHKERLKQLRVMVDVMTMSATPI
                     PRTLYMALSGARDMSIINTPPTNRAPIKTFVGEYKLPLVRTAILHEMERGGQVYFVHN
                     RVENIEQIAAEVKMLVPEARIAIGHGQMGERDLENVMLAFLAHEYDVLVCTTIIESGL
                     DIPNVNTIIINDADKLGLAQMYQLRGRVGRSEVQAYAYCLYKPSKVLSEQAQGRLKAI
                     REFTSLGSGYQIALRDMEIRGVGNILGAEQHGHMISVGFDLYCKLLEESVAELKGEAV
                     AQDYDTAVDINVTAYIPESYIQDNEQRLVEYKRLADVKTERELSMLMTEWKDRFGNIP
                     KETEQLVKVVRLRLLAAAANVQSIKPDMQGMRLYVPFRLQQWMPLQARLPKHLGSRTT
                     YKPGIAGGQGSSPYILVKAQGDEPEEQLNLLEDLLQSMATSVEVNTRPA"
BASE COUNT          726 a         1016 c         1062 g         1134 t
ORIGIN      
        1 ccttccagca cataagtgtg ggcccggttc accagggcca ctgccagatt agggctcaat
       61 tccagagctt tgttggcgtc tgccatggct tctttagttt tgcccaggcg gttataggca
      121 tgggagcggt tcacgtaggc gatagccatt ttgggttcca gctcaattcc tttgctggca
      181 tcatcgaggg ccttttgcac ctgcccgagg gcaagttccg cccaggagcg gttgatataa
      241 gccataccca gtttgtcatc gaggcttaag gctttatcgc agtcggcaat ggcccggcga
      301 tagtgtttgc gcatcatatg aatattggag cgattggcat aggctctggc ataaccggga
      361 tgaagtttaa tcgcctcatt gaacttggct tctgcttcct gtcccttgcc ctctcctgac
      421 agccggatgc cttccttgag aagggcaaaa gcttcgggat tggtcttgcc cttcggggcg
      481 gctttctctg ccggcttaga cttggcgcct ttcttttccg ctgcttgtgc ttgtaaagaa
      541 gtggtgagcg ttcccgcaga aactacagtg atagccaaca tcattgagct cagtcttcta
      601 tttctttcga gcattttgcc tctcctattg ccgcgtggtt attatctcac agccataccg
      661 gcctaaaact atttgacatt tgcctggggc acactggctg tgcttgaggc tttgcggaat
      721 tcgtcggaaa agacgatttt ggccgacttg cgcttgttaa ccagccagcg ggttttggct
      781 gcttccatat tttgggcgac gaatttttcc ttgagctgat ctttgatttc atcgaagctg
      841 aaggtgccgg ccggcacttt ctccgtcaat ttgatgacgt ggtaaccgaa gttggtttca
      901 acgggagtaa gagccagttg gccgggtttg aggtacttca cggcatctag caaagctttc
      961 tgatccggtc ctgcctgggc ggcaactacg tctatataac ctagatcgcc gccgtttttg
     1021 gcttgtctgg cttgcatatc ttccgtgttg tcgttggcca gggatttgaa gtcttcacct
     1081 ttattggctc tggctagaag ttcctgggcc agtcgcatct tctgttgctt caccaccagc
     1141 acttccttgt ccagatcggc gtcggacatg gttggatttt gtttcttcaa ctgggtcttg
     1201 atgctctcca tgggtggtgc atcgaccctg ggggcagcca gaacaatatg ggagagtctc
     1261 accttctcac cgtgtttgaa ggcttctttg ttcttttgat agaaagctaa tagctccttg
     1321 tctgaggcca cgctttccct ggcgatcttt tgcatgagca taatcagcat ttcgccctgg
     1381 atgagatctt cctttatctc ttccggagtc tgatcgctca tctccagcat cttcttatat
     1441 tttggtaggt cttttacctt caaataattg ttgtaagccg gcttgtagaa gttctgtgct
     1501 ttggcttcgg caagcaatag ctcacggttg ataagctcgg tgagcaattg ctgctcaatc
     1561 aacttggcac caactttaaa agcgagacca agatcgatta cttgcttgtc gaattcagct
     1621 tccgtcagtt tcttctcttt caggaatttg tcgagactct tgccttccag tgattgcggt
     1681 ttgcgtgccg cctccagaag ctttttcttc tcttcggcgg tgaggcttac accggctgcc
     1741 gtagcctgtt tcaccagctc ttgcacctgc atgggttgca tgcccagcac ggcttgcagg
     1801 gagcctatgg cttctctgta ttcccttttg aactgagaaa tggtaaccgg cgtaccgtcc
     1861 acggtgcaaa tcaccatagt gggcggtaag gtggccaggt tcacggcatt gggaagtttg
     1921 acattgccgc cggtgacagc cggttcctta gcggtagcat catccggtgt attggtctga
     1981 gaagcctgtc cggtcttgtc ttcgcttttg gccggttcgg tcgtggtacc gctgccggtg
     2041 ctgctcgttt gctcacctgt tttttcaccg ccgctggttt cctgcttgct attgcaaccg
     2101 caaagcagca gcgaaccggc gcaaatggcc agtgtcacaa gggtgaattt gccgcctgcc
     2161 gtctgtggac ggcatagcct ggactgttct ctgcttttgg atgggtagcc tgcaattttt
     2221 ccatcttcca aattgcttac ttttccggtc acttgtttta cctcaggcgg gtcttgtgtt
     2281 tacttcaacg ctggttgcca tggattgcaa aagatcttcc agcagattga gctgttcttc
     2341 cggctcatcg ccctgcgctt tcaccagaat gtaggggctg gagccctgtc cgccggcgat
     2401 gccgggtttg taggtggttc ttgaaccgag gtgtttaggc agtctggctt gcagaggcat
     2461 ccactgctgc aggcggaaag gaacatagag gcgcataccc tgcatatcgg gcttgatgga
     2521 ttgtacgttg gcggcggcgg ccaaaagacg caagcgcacc actttcacca gttgctcggt
     2581 ctctttgggg atgttgccga aacgatcttt ccactcggtc atgagcatag acaactctcg
     2641 ctcggttttg acgtcggcca gtcgtttgta ttccacaagg cgttgttcgt tgtcctggat
     2701 gtagctctcc ggtatgtagg ccgtgacgtt gatatcaacg gcggtatcat aatcctgagc
     2761 aacggcttcg cccttcagtt ctgccacgga ttcttccagg agcttgcaat agaggtcgaa
     2821 gcccaccgag atcatatgtc cgtgctgttc ggcaccgagg atgttgccta cacctcttat
     2881 ttccatgtct cgcagggcta tttgatagcc ggaaccaagg gaggtaaatt cgcgtatggc
     2941 cttcagtcgt ccctgggcct gttccgacaa gaccttggat ggtttgtaga ggcagtaggc
     3001 ataagcctgt acttccgagc ggccgacccg acctctgagc tggtacatct gggccagacc
     3061 gagtttgtcg gcgtcgttga tgataatggt attgacgttg ggaatatcga gacccgattc
     3121 gataatggtg gtgcaaacca gcacgtcata ttcgtgggcc aggaaggcca gcatgacatt
     3181 ttccagatct cgttcaccca tctgtccatg accgatggcg atacgggctt ccggcaccag
     3241 catcttcact tcggcggcga tctgttcgat attttctaca cggttatgaa cgaagtagac
     3301 ctgaccgcct ctttccattt cgtgcagaat ggccgtgcgc accagaggca atttgtattc
     3361 gcccacaaag gtcttgattg gtgcccggtt ggtgggcggc gtgttgatga tggacatatc
     3421 cctggcgccg gatagggcca tgtagagagt cctcgggatg ggcgtggcgg acatggtcat
     3481 tacatccacc atgacgcgca attgcttcag tctttctttg tgtgccacgc cgaatcgctg
     3541 ctcttcgtct atgactacaa gaccgaggtc tttgaaggca atgtcttttt gcagcatgcg
     3601 gtgggtacct atcaccacgt cgcattcacc cgatgtgagg cgtttggcta cttccctttg
     3661 ttctttggcg gtgcgaaaac ggctcaaaag accaactttg accgggtagg gagcaaagcg
     3721 atcggccata ttgttgtagt gctgctgggc caaaatggtg gtgggcacga ggaccgccac
     3781 ctgcttgccc gacattacgg ttttgaagac gccgcggatg gctatttccg ttttaccgaa
     3841 gcctacgtct ccgcagatga ggcggtccat gggtttgctc gattccagat cgttcttcac
     3901 gtcctggatt gcctgccatt ggtccggcgt ttcctcgt
//
LOCUS       DAOQNZ010000177         3931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10623_length_3931_cov_3.917699, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000177 DAOQNZ010000000
VERSION     DAOQNZ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10623_length_3931_cov_3.917699"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2658)
                     /locus_tag="PKW73_04430"
     CDS             complement(<1..2658)
                     /locus_tag="PKW73_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72557.1"
                     /translation="MVNFKCGLTLEELERETGKLVSDLAFYARRPVNIQLVAGKHIAF
                     TYGTDKLKKPIPVVMNPQVLENIRNKERALKVWRGIGFHELSHHLWPADEQYKAANTE
                     GFQHLFNLVDDEQNERRGRALDPSWGACFQSVCAHIFPTRDNARVSTGLTDGGVEEKK
                     PWGVAADKVYSKRWGTFAYHFRRHIPGCTDPVVAKALALIPADFKELPKEELFDLTRK
                     IHQTLCTGIELSEQAPPVEPEQQPEEEEKKEDEQEQETQEDKPIFPSPGWNIKRLLKS
                     KWMLVPLGLFIVGWSALFLQKDLDFWLQVALAGGMALIGITVFLFLRRSLIKSRLAAL
                     KAKGAAADKRPIKKAFIGLLCTLILSICAYLYFVKGLRLPIEWLYTGGTVALFAGLLH
                     IGNKLSKHADESGKPLSWLTYLLMTPGLLISLAGLFFVIRMYGLNWYWTGAIVSAAGF
                     IALCLLSIMTGSGKSTERNRTYSLSERVHRGSGPLMRLSLRVLSFFSHILWLLVGKPL
                     FLVLSFLYKVIARCLLYLWSLVTRFFRFIWHYIRRAFWKLKPYLVRWWRNPYFRLAVI
                     AMPIAAIGIIIYALVVKAASLSWWLLTLLILLLLLLLALLYFFRRQISRFILNELFMP
                     MPDLMQMFIQPPLDMTTDWFVQIDDVAEVEADQQVVDEILPETFALASQLRKYLVECG
                     SALVDRDDQPDGHDLIDEAELALLGEPNIFVDDDRYTRTSVHLEVALDCSGSMASPTS
                     SLPAGEKFRLGKLFAMVIEQAVVNLPGVSARFWGFTSDTIFDCGNAGERKISGLKCGG
                     GNNDAAMLWKMSQSARQSGKEVKILLMLSDGQPSECSWLSLRNLVLKLEQEGMIPWNF
                     ALDVIDTPAFERFFTDLVGQSKDEAVLTMG"
     gene            complement(2701..3801)
                     /locus_tag="PKW73_04435"
     CDS             complement(2701..3801)
                     /locus_tag="PKW73_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA72558.1"
                     /translation="MSEVISTGDTLVVHWETNPVSQSPNAVKSNKTDSGRTKKIILVA
                     DPSKGVQPQPGEAWICSVDRISNPKSESHGAIFVRPVSRKVEYHFKGVYIDPVKAQLI
                     ATILQDPRRNLMLEGDQGVGKSTIASAIAKTLGWEYRKVSGGLIKKFVFMLGRYIPTS
                     DSSTLNFRWADSKLVASLREAENNPRRKYLLMIDEFTRIDEDARDALLDIIEGSVRVL
                     RLPTGEEISVGDNVVFMAAGNVGEGFTLRREDAAAKDRWAIVKILVMPQEEELAHCMR
                     LYPSCNRAELDRALTIVNQVRTARRDTKMRLSKTVSTRGAEALAMFLNSGFPIEVAME
                     TAVVNQYSGSFDDPNSEAGRVYKLVMDALSKK"
BASE COUNT          919 a         1004 c         1109 g          899 t
ORIGIN      
        1 gcccatggtc agcacggctt cgtctttgga ttgcccgacc aggtcggtga agaagcgctc
       61 aaaggccggg gtgtcgataa cgtccagagc aaagttccag ggaatcatgc cttcctgctc
      121 cagtttgagt acaaggttgc gcagtgacaa ccaggagcat tccgagggtt gtccgtcgga
      181 gagcatgagc aggatcttca cttccttgcc gctttgcctg gccgactgag acattttcca
      241 gagcatggcg gcgtcattat tgcccccacc gcatttgaga ccggaaatct tcctttcacc
      301 cgcgtttccg cagtcgaaaa tggtgtctga ggtgaagccc cagaaacgag ccgatactcc
      361 cggtagattt accaccgctt gttcgatgac catggcgaag agtttgccca gacgaaattt
      421 ctcgcctgcc ggtagagaac tggtggggct ggccatggag ccggaacagt ccagagctac
      481 ttccaggtgt acggaagtgc gcgtgtaacg gtcatcatcc acaaagatgt tcggctcacc
      541 cagaagagca agctcggctt cgtcaattag atcgtgcccg tccggttgat catcccggtc
      601 gacaagggcg ctgccgcatt caaccaggta tttgcgcaac tgactggcga gagcgaaagt
      661 ctcaggcaag atttcgtcta cgacttgctg atcggcttcc acttcggcaa catcgtcaat
      721 ttgcacgaac cagtcggtgg tcatatcgag gggcggctgg ataaacatct gcatcagatc
      781 cggcatgggc ataaaaagct cgttcaaaat gaagcggctg atttgccggc gaaagaagta
      841 gagcagagcc agaagcagaa gcaggagcag tatcagcagt gtcaatagcc accaactgag
      901 ggatgctgct ttgacgacga gagcgtagat gattatgcca atggcggcaa tcggcatggc
      961 aattacagcc aggcgaaaat agggattgcg ccaccatctg accagatagg gtttaagctt
     1021 ccagaaagca cgccttatgt aatgccagat gaaacgaaag aatcgcgtca ccaggctcca
     1081 caggtagagc aggcagcgtg cgattacctt gtagagaaag ctgagcacca ggaagagcgg
     1141 cttgcccact agtaaccaga gaatatggct gaagaaggaa agcactcgga gcgacagcct
     1201 catcagcggt ccggagccgc ggtgcactct ttcactgagg ctgtaagtgc ggttcctttc
     1261 ggtgctcttg cctgaccccg tcatgatgga aagcaggcag agggcgatga agccggccgc
     1321 cgagacgatt gcaccggtcc aataccaatt cagaccgtac attcggatta cgaagaagag
     1381 cccggcaagc gagatgagca agcccggcgt cattagtaag taggttagcc agctaagcgg
     1441 cttgccgctt tcatcggcat gtttggaaag cttgttgccg atatgcagaa gaccggcgaa
     1501 tagtgccact gttccgccag tgtaaagcca ttcaatgggc aggcgcagtc ccttcacgaa
     1561 gtacaggtag gcgcaaatgg agaggatgag agtgcagaga agaccaatga aagctttttt
     1621 gatcggacgt ttgtcggcgg cggcaccttt tgcttttagc gccgccagtc ttgattttat
     1681 tagtgagcgg cgcaggaaca ggaagacggt gatgcctatc agagccattc ctccagccag
     1741 cgctacctgc aaccagaagt ccaggtcctt ttgcaaaaat agggccgacc aacccactat
     1801 gaagagcccg agcggcacaa gcatccactt tgacttgagt agtcgcttga tattccaacc
     1861 aggcgacgga aatatgggct tatcttcctg ggtctcttgt tcttgctcgt cctctttctt
     1921 ctcctcttct tccggctgct gttctggttc caccggtggt gcttgctccg agagttcaat
     1981 gccggtgcaa agtgtctgat gaatcttgcg agtcaaatca aacagttctt ctttcggcaa
     2041 ctccttgaag tcggcaggaa ttagagccag cgcttttgcc accaccgggt cggtgcaacc
     2101 gggaatgtga cggcggaaat ggtaggcaaa ggttccccag cgtttggagt agaccttgtc
     2161 tgctgctaca ccccagggct tcttctcttc cacgccgccg tcggtaagtc cggtagaaac
     2221 cctggcatta tcgcgggtgg ggaagatatg agcgcagaca ctctggaaac aagctcccca
     2281 tgagggatca agcgctcttc cccttctttc gttctgttcg tcatcgacca gattgaagag
     2341 atgctggaag ccttcggtgt ttgcggcttt atattgttca tcggccggcc acaaatggtg
     2401 ggatagctcg tggaagccga tgccgcgcca gactttaaga gcgcgctcct tgttgcgaat
     2461 attttccagt acctggggat tcataacgac cggaatcggc ttcttgagct tgtcggtgcc
     2521 gtaagtaaaa gcaatgtgtt tgccggcaac tagttgtatg ttgaccggtc tcctggcata
     2581 gaaagccagg tctgatacaa gtttgcctgt ttcccgctcg agttcttcga gggtcaggcc
     2641 gcatttaaag ttgaccatgg ctgttcctga aagagagcgc agagcgcgtt atagataggc
     2701 ttactttttg gacagagcgt ccataaccag cttgtaaacg cggccggctt cggaattggg
     2761 gtcatcgaag gagccggaat actgattgac cacggcggtt tccatggcta cttctatggg
     2821 aaagccgctg ttgagaaaca tggctagtgc ttcggcgccg cgtgtggaaa ccgttttgga
     2881 aagacgcatc ttggtgtccc gacgcgcggt gcgcacctgg ttgactatgg tgagagcacg
     2941 gtccaactcc gctctgttgc aggaaggata aagccgcatg caatgggcaa gttcttcctc
     3001 ttgcggcatg accaggatct tgacgatggc ccagcggtct ttggcggcag cgtcttcacg
     3061 gcgaagggtg aaaccttccc cgacgttgcc ggcagccatg aagaccacat tgtcaccgac
     3121 ggaaatttct tcgccggtag gtaacctcaa tacacgcaca ctgccttcga tgatgtcgag
     3181 cagagcgtcg cgggcgtctt catcgatacg agtaaactca tcaatcatga gcagatattt
     3241 gcggcgcggg ttgttctctg cctcgcgcag ggaagcaacc aatttggagt cagcccagcg
     3301 gaagttcaaa gtgctgctgt cggaggtagg aatgtaacgt ccgagcataa agacgaattt
     3361 cttgatgaga ccaccgctca ctttgcgata ttcccaaccg agagttttgg cgatggccga
     3421 cgcgatggta gacttcccaa cgccctgatc accttccagc atgaggtttc ggcgtgggtc
     3481 ttgcaagata gtggcaatca attgcgcttt cacgggatcg atatagacgc ctttgaagtg
     3541 gtactccacc ttgcgagaaa ccggacgcac aaagatggca ccatggctct cggatttagg
     3601 attgctgatg cggtctacgc tgcagatcca ggcttctccc ggttgcggct gcacaccttt
     3661 gctggggtcg gctaccagga tgattttctt ggtgcgacca ctatctgttt tgtttgattt
     3721 gacggcgttt ggagactggc ttaccgggtt ggtttcccag tgaacgacca gggtgtcgcc
     3781 ggttgaaatt acctcagaca taaagaaaac tcctctgaaa tttagataag tcgagccagg
     3841 caaagacgag agagacaggc ttccaccggt gcaactggaa agatgcaacc gctaaatcgg
     3901 aacagcgctc actaccaaat ttgaatggcg g
//
LOCUS       DAOQNZ010000178         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10681_length_3918_cov_3.794978, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000178 DAOQNZ010000000
VERSION     DAOQNZ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10681_length_3918_cov_3.794978"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1335)
                     /gene="mqnE"
                     /locus_tag="PKW73_04440"
     CDS             complement(136..1335)
                     /gene="mqnE"
                     /locus_tag="PKW73_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNA72559.1"
                     /translation="MSSLSFPAGISELAVDSGLQDIAEKVYAGQRLTREDAIRLYQSD
                     DLLTVGALAEYARRRTAGAGKERYVYYIHNMHLNPTNFCVETCRFCSYANPAERGRAY
                     TWSVDQVLAEAGKGAALGINEIHMVGGLNPACDLAYYEDILRAMRAKYPQIHMKAFTA
                     VEIEYLANLEGLSYEAVLKRLVEAGLGSMPGGGAEIFDDQVRERMAVKKTPGPVYLEI
                     HGIAHGLGLKSNATMLTGIGESIENKVDHMLMLRGQQDLSGGFQCFIPLKCYYEGTDI
                     EAEVKEPTAADLLKDVAVSRLLLDNFPHIKAYWIQLGVELAQLALSFGADDMDGTIGQ
                     EKITHAAGAKTPLQLAKERMEELISTSGYQAVERDTIYNIKAVARTEPRLVETPMSER
                     LSAILAV"
     gene            complement(1484..2071)
                     /locus_tag="PKW73_04445"
     CDS             complement(1484..2071)
                     /locus_tag="PKW73_04445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA72560.1"
                     /translation="MQDISPKLVRPVPKLNTGHCVLRLPEPAESPAVVAFLKKNRDHL
                     ANAGPAWPDDYLTEAYWVNQLTTNLLEFEEERSVRFFIFERSNPEAVVGYANISNIVK
                     GAFHAGILGYGIDKDKEGRSMMKEALQSVITYGFSALNLHRIMANYQPVNERSGGLLR
                     SLGFTVEGYARDYLLINGRWRDHVLTSITNPNWMP"
     gene            complement(2301..2954)
                     /locus_tag="PKW73_04450"
     CDS             complement(2301..2954)
                     /locus_tag="PKW73_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72561.1"
                     /translation="MIVISNVLLFVRSLSELVRPEVLAEVNSGKMPMPNLGEIAGNQD
                     KMVSLKDKFACDHGANLSNLKRRATGEIATPDGLSNMERHRKAMLRWFSSERESPEQA
                     LNERMKQVEQRRLNSLPSLPSLPSSEDSFLAWTSEDEYLFDRPVKQQWKDYDDSLNQL
                     ATVVEYINEGPSAFDAQPAQKFEEGSDCDLEDMLSKIDVGTAYHASSSLMMPNYWRD"
     gene            complement(3190..>3918)
                     /locus_tag="PKW73_04455"
     CDS             complement(3190..>3918)
                     /locus_tag="PKW73_04455"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HNA72562.1"
                     /translation="VELAEEVLALSGGAESETGAGTVLAMVDGSLIPWSLEKMPRPYM
                     EDYLTRLEVLMCRLRLKGIAVVGYVSHSRSADLINALRVTICPYALSHCQTNCGHLNE
                     ESFPCSAIWPLSDRLLYQSILKPGETSSVFESAASVVKLMPEADATCFAYFRNEMEVA
                     RLEFPRWIYQDSALFEFLLAGVKAQCEKGFGYPVVLAEAHHQAVIRGPEREQFFSLIA
                     QRLLTIGARTAVSPKEANKRRGFI"
BASE COUNT          927 a         1092 c         1009 g          890 t
ORIGIN      
        1 cgacatcatc aaggccgtag acggagtggt gaccgacaac gtcaacacct tcaatcaaat
       61 gctcaagcgc aagcagccga acgaaggcgt gcaaatgatc attcagcgcg gcgctaaaat
      121 cggtcagttt gtactctaaa ccgcgagaat ggcgctcagt cgctcggaca ttggcgtttc
      181 caccaggcga ggctcggtgc gagccacggc tttgatattg tatatggtgt cacgttccac
      241 agcctgataa ccgctggtgg aaatcaactc ttccatacgt tccttagcca gttgcagagg
      301 agttttggct ccggcggcgt gggtgatttt ttcctggccg atggtgccgt ccatgtcatc
      361 agccccgaag gaaagggcta gctgggccaa ctctactccc aattggatcc aataggcttt
      421 gatatgtgga aaattatcga ggagcaagcg ggagacggcc acatctttga gcaagtcggc
      481 ggcagtcggc tccttcactt ccgcttcgat atcggtaccc tcgtaataac acttgagggg
      541 aatgaaacac tggaaaccac cgctcaagtc ttgctgcccg cgcagcatca gcatatggtc
      601 tactttattt tcgatggatt caccgatacc ggtgagcatt gtggcattag acttgagccc
      661 aagcccatga gcgattccgt gtatctcaag ataaaccgga ccgggagttt tcttaacagc
      721 catgcgctcg cgtacttgat catcgaagat ttcagcaccg ccgccgggca tggaacccag
      781 accggcctcc accaggcgct tgaggacggc ttcgtagctc agcccttcca ggtttgccag
      841 gtattcaatc tctacggccg taaaggcctt catgtgtatt tgcgggtact tggcccgcat
      901 ggcgcgcaga atatcttcgt agtaggccag atcacaggca gggttcaggc cgcccaccat
      961 gtgaatctca ttgattccca gcgccgcccc tttgcccgcc tcggccagca cttgatccac
     1021 gctccaggta taggcgcggc cgcgctcggc aggattggca taagaacaaa agcggcaggt
     1081 ctccacacaa aaattggtgg ggttcaagtg catgttgtga atgtaataaa cgtaacgctc
     1141 tttaccggca ccggcagtac ggcgacgggc gtactcagcc agggcgccca cggtcagtaa
     1201 atcatccgat tgataaaggc gaatagcgtc ttccctggtc aatctttgac cggcatagac
     1261 cttttctgca atatcctgca gccccgaatc gacggcaagc tcactgattc cggcagggaa
     1321 ggataagctg gacatagaca ccccttgata tctctcaaac atctctcaaa aacggatgcc
     1381 taaatcctag cactaaagca ggcagattgc cccctggagc cctttcctga gaggtagtca
     1441 gcgcaacaat ttaaagccct ttaagctgcc tggagcagaa agcctatggc atccagttgg
     1501 ggttggtgat ggaggtgagg acatgatccc gccagcggcc gttgatgagc aggtagtccc
     1561 gggcataacc ttccaccgtg aagccgaggc tgcgcaacaa gccgccgctg cgttcattca
     1621 ccggttgata attggccata atgcgatgca gattgagggc tgaaaaacca tatgtgatga
     1681 ctgattgcag ggcttccttc atcatggaac gcccctcttt gtctttgtct atgccgtagc
     1741 ccaaaatacc ggcatggaag gcacccttga ctatgttgct gatattggca tagcccacta
     1801 ccgcttcagg attggagcgc tcgaatataa agaagcgtac ggagcgctcc tcttcaaatt
     1861 caagcaaatt tgtagttagc tggttgaccc agtaagcttc ggtcaggtag tcatctggcc
     1921 aggccggacc ggcattggcc agatggtccc ggttcttctt gagaaaggct actacggcag
     1981 gagactcagc cggttcaggc aggcgcaaaa cacaatgccc cgtattcaac ttgggtaccg
     2041 gtcttaccaa tttcggcgaa atatcctgca tttatctccc cccaagtctt tttaagaggc
     2101 tggctgaaca ctgaggatta tacccacagc tgtaaagcaa atactggcag gaagtgtgtg
     2161 gactgaaaga aagactcgtc taattttgcc cgcaccgata ctaccggcgg tgcaattggc
     2221 gacgcgccct gtctgggctg taaccaccgg cagtgccgac agtaacaacc ggcagcgccg
     2281 gcagaaagct gtctagagag ttagtctctc cagtaattcg gcatcatgag gctgctggaa
     2341 gcatgatagg cagtgccgac atcgatcttg gagagcatat cttccaggtc gcagtcggag
     2401 ccttcctcaa acttttgagc gggctgagca tcgaaggcac tcggtccttc atttatgtac
     2461 tcgaccacgg tggccaattg attgagacta tcgtcgtagt ctttccactg ctgtttgacc
     2521 ggtcggtcaa agagatactc gtcttcggaa gtccaggcca ggaagctatc ttcgctcgat
     2581 ggcagggaag gaagggaggg caggctgttc aagcgcctct gctcgacctg cttcattctc
     2641 tcgttcaaag cttgctcggg actttctctt tcggaagaga accaacgcag catggctttg
     2701 cggtgccgct ccatgttgct caagccgtcg ggagtggcta tttcgccagt agcgcgccgc
     2761 ttcaagttgc tcaaattggc accgtggtca caggcaaact tatctttgag gctgaccatc
     2821 ttgtcttgat tgccggcaat ctcacccaga ttcggcatgg gcattttgcc gctgtttact
     2881 tccgccagga cctcaggtct tacaagttcg ctcagacttc tgacaaaaag caaaacatta
     2941 gaaatcacaa tcacttacct cgccatttcg gcgccttatt gcgcctaccg cctcgccctc
     3001 aaagatttga gttggacgaa gattaagtca ttctcacacc cttgtcaagc gacgctagcg
     3061 tatttccacg ctaccttttc gcctctcagt tgcaagggca acggcagact acttccatgg
     3121 agttgagaat atagcaaagt catatacagg gcacataaga atcatccaac ccttaccggc
     3181 aatcgcttcc tatataaagc cccggcgctt gttggcttcc ttggggctga ccgcagttct
     3241 ggcccctatg gttaagaggc gctgggcaat caagctgaaa aactgctccc tttccggtcc
     3301 cctgatgaca gcctgatggt gtgcttccgc caacaccacc ggatagccga aacctttttc
     3361 acactgggct tttaccccgg ccaagagaaa ctcaaaaagg gcgctgtcct gatagatcca
     3421 gcgaggaaac tccagccggg ctacttccat ctcatttcgg aagtacgcaa aacaggtggc
     3481 atccgcctcc ggcattagtt tgaccaccga ggcggcactt tcaaagacac tggaagtctc
     3541 tcccggcttc agtatggact gatagagcag gcggtcgctc aaaggccata tggccgaaca
     3601 gggaaaactc tcctcattga gatgaccgca attagtctga caatgactca gggcataggg
     3661 gcagatagtt acccgcaagg cattgatcaa atcagcgctg cggctgtggg agacataacc
     3721 gaccacggca atacccttga gacggaggcg gcacatcagc acttccaacc gggtcagata
     3781 atcctccatg taagggcggg gcattttctc cagcgaccag ggaatgagcg aaccgtccac
     3841 catggccaaa acggtcccgg ccccggtctc gctctccgca ccgccactga gggccagcac
     3901 ctcttccgcc agttctac
//
LOCUS       DAOQNZ010000179         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10682_length_3918_cov_3.669169, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000179 DAOQNZ010000000
VERSION     DAOQNZ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10682_length_3918_cov_3.669169"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="PKW73_04460"
     CDS             <1..1780
                     /locus_tag="PKW73_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72563.1"
                     /translation="YADTVVPVTSVAPVFQSFRPLSLSGYSDASALAAAKSSDSALLG
                     APGSRAEVSPVLDKNRRVTDLIQAGVMRRIESTRPPLAEPLKEGEVPQVLITYADTAQ
                     DQSRTPDFIVRKDGRIEAAGDFDSTARKQVVIQVERQAGSTANPEQAQQKSIDELVQY
                     VHGRMSANSEVARRGLDINDEYGLMSDSLTKKIAAGQLKAADSAVNANADRSGYSPES
                     QRMMSDMNRFTPGSSGSMSPREVGDYFPRRDVGQQRDETNKVAHAKNAVAGMFNPDKT
                     APYETVRVLPPPMGLAIGRYGLTHRNFSTWFSLEGEDEYNEGNIAGIMAKLAKKGKVS
                     KEFAAKFQDKNFAAKFVGAMKRMEEGKSLTGEEMRTLFPKELQERVATDTVDKMLKDA
                     GGDVGKTALALHLGKEPGQLTGEDLNNKANKDYMSGAVKLAQLSEMRRELGQGDRVDW
                     QVSPEGNMMKAKIVKAGLDVARNMGTEGRCAEGVQVALSKVGMNEFMGSGDGWAMRHA
                     FLKSDEWRVTNDPTRATAFVRSWAPSVRAEYGGRNLGHVGLLHMENGRLIESSDHKTE
                     HLVINSRYNQTIYFEYVGKKPRTMEA"
     gene            complement(1810..3156)
                     /gene="hisS"
                     /locus_tag="PKW73_04465"
     CDS             complement(1810..3156)
                     /gene="hisS"
                     /locus_tag="PKW73_04465"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNA72564.1"
                     /translation="MAVDSQISSAPQSGMRDFLPREVRLRDWATAIITATYEQFGFTK
                     IETPCLENIALLKRGDGGENLQLIFEVLKRGEKLDRVLGSIKDSENNGGESRHSKSLK
                     EVRQDLSDMGLRFDLTVPLVRFYSHNQQNLPSPFKSIQIGSVWRAESAQQGRFRQFTQ
                     CDIDIFGVKSELAEIELLQATSEALVKLGFEGFTICINDRRMLSSLARYCGFEEERFE
                     SVFIALDKLDKIGLDGVGHELAGQGHKEEAIANLRSLLVTMAEATGTSEQQLETLAGK
                     IAADSAVSQALKNIMSVVMKNADGRFNVQFEPSLVRGMGYYTGPIFEIKYPGYNYSVA
                     GGGRYDKMVGKMSGRDVPACGFSIGFERIIGILMDEGFEPPAAMERVALIFDENRDQL
                     PDVMAAAANLRARGMAVVVQSKKKDMRKQIDQLPAQGIAKLCLFKGDVENLEIKPL"
     gene            complement(3476..3730)
                     /locus_tag="PKW73_04470"
     CDS             complement(3476..3730)
                     /locus_tag="PKW73_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72565.1"
                     /translation="MNQSMKAGNVSPEGTGATSATVAAGDCHCQKDKNPVCPTCSTVL
                     KGGLKYENICTTCNPQFLNRCGGCQRLLYDCMCIRPHTWE"
BASE COUNT          838 a         1044 c         1121 g          915 t
ORIGIN      
        1 ctatgccgac acggtcgtgc ccgtaacgtc ggttgctcca gtatttcaga gtttcagacc
       61 gctttccttg agcggctatt ccgatgcttc cgcactggct gcggccaaat ccagcgactc
      121 ggcactgctt ggtgctcccg gcagccgagc tgaggtctcg ccggtgcttg ataagaaccg
      181 ccgcgtcacc gaccttattc aagccggtgt gatgcgtcgt attgagtcta cccgtcctcc
      241 tctggccgaa cctcttaaag agggcgaagt accgcaagta cttatcactt atgccgacac
      301 tgcccaagat caaagccgca cccctgattt tattgtgcgc aaagacggac gtattgaagc
      361 cgccggagat tttgacagca ctgccaggaa gcaggtagtg atccaggtgg aaaggcaagc
      421 cggcagtacc gctaatcctg agcaggcaca gcagaagagc attgatgaac tggtgcagta
      481 tgtgcatgga cgcatgagcg ctaattcgga agtggctcgt cgcggtctgg atatcaatga
      541 tgaatatggt ttgatgtccg attctctcac taaaaagatt gccgccggtc agctcaaggc
      601 tgccgattca gccgtcaatg ctaatgccga tcggtccggc tatagccctg agagccagcg
      661 catgatgtcc gatatgaatc gcttcacccc tggttccagc ggctccatga gccccagaga
      721 ggtaggcgac tatttcccca ggcgcgatgt gggccagcag cgcgatgaaa ccaacaaggt
      781 tgcccatgcc aaaaatgccg tcgccggtat gttcaatcct gataaaactg ctccctacga
      841 gactgtgcgt gttttgccgc ctcccatggg gcttgccatc ggtcgttatg gcttgactca
      901 cagaaatttc tctacctggt tttccctgga aggtgaggac gagtacaacg agggcaatat
      961 cgccggcatc atggctaaac tggccaagaa aggcaaagta tccaaagagt ttgccgccaa
     1021 gttccaggat aagaacttcg cagccaagtt tgttggtgcc atgaagcgca tggaagaagg
     1081 taagtccctc accggagagg aaatgcgcac cctctttccc aaggaattac aggagcgcgt
     1141 agccaccgat accgtcgata agatgctgaa agacgccggt ggtgacgtgg gcaagacggc
     1201 tctcgctttg cacctgggca aagaacccgg gcaacttacc ggcgaagatc taaacaacaa
     1261 ggccaacaaa gactacatgt ccggtgccgt caaattggca cagttatcgg aaatgcgccg
     1321 ggaactggga cagggagatc gtgtagactg gcaagtttcg ccggaaggca acatgatgaa
     1381 ggcaaagatc gtcaaagccg gtctggatgt ggcacgaaat atgggcaccg aaggcagatg
     1441 cgccgaaggc gtgcaggttg ctttgagcaa ggtcggcatg aatgagttca tgggctcggg
     1501 cgatggttgg gccatgcgcc atgccttcct caagtctgat gaatggcggg tcaccaacga
     1561 tcctactcgt gctaccgctt ttgttcgctc ctgggctccc tccgtccgtg ccgaatacgg
     1621 tggtcgcaat cttggtcacg tgggtctttt gcacatggaa aacggtcgcc tgatcgaatc
     1681 atccgatcac aaaaccgagc atctggtgat caattcccgc tacaaccaga ctatctattt
     1741 tgaatacgtg ggcaaaaagc cacgcaccat ggaagcctga gtagccgctg tcgcagcgta
     1801 gagccgtctc tagaggggct tgatctccag gttttccaca tcgcctttga ataggcagag
     1861 cttggctatg ccctgggccg gtagctggtc gatttgtttg cgcatgtctt ttttcttgct
     1921 ctgcactacc actgccattc cacgagcgcg caggttggcg gcggcagcca tgacgtcggg
     1981 caactgatcg cgattttcgt cgaatatgag cgccactctc tccatagcgg cgggcggctc
     2041 aaaaccttcg tccatcaaga tgccgatgat gcgctcgaag ccaattgaga agccgcaggc
     2101 cggtacgtct ctgccggaca ttttgcccac cattttgtcg tagcgcccgc ctccggcgac
     2161 cgagtagttg tagcccgggt atttgatttc gaagataggg ccggtgtaat agcccatgcc
     2221 gcgcaccagg gagggttcga actgaacgtt gaaacggccg tcggcatttt tcatgactac
     2281 cgacatgatg tttttcagcg cctggctgac ggcgctgtcg gcggcgatct tgccggctaa
     2341 tgtttccagt tgctgctcgc ttgtgccggt ggcttcggcc atggttacaa gcagtgatcg
     2401 caggttggcg atggcttctt ccttgtgtcc ctgaccggct aattcgtgac cgactccatc
     2461 gagacctatt ttgtccagtt tgtcgagggc gatgaagacg ctttcgaagc gctcttcctc
     2521 aaagccgcag tagcgtgcca ggcttgaaag catacgcctg tcgttgatgc agatggtgaa
     2581 gccctcaaaa cccaatttga cgagagcctc ggaagtggct tgcagcagtt cgatttccgc
     2641 cagttcgctt ttgacgccga agatgtcgat gtcgcactgg gtaaactggc gaaaacgccc
     2701 ttgctgagcg ctttctgccc gccagaccga gccgatttgg attgatttga aggggctggg
     2761 aagattctgc tggttgtgac tgtagaagcg caccaggggc acggtcaggt cgaagcgcag
     2821 acccatgtcg ctcaagtcct ggcgcacttc tttcaagctt ttgctgtgcc ggctttcccc
     2881 gccgttattt tcagaatctt tgatggagcc caagacacgg tcaagcttct cgcctcgctt
     2941 caagacttcg aagatcagtt gcagattctc tccgccgtcg cctctcttga ggagggctat
     3001 gttctcaagg cagggagtct cgatttttgt gaaaccaaac tgttcatagg tggcagtgat
     3061 gatggctgta gcccagtctc tcagccgtac ctcgcgcggt aaaaaatcgc gcatgccact
     3121 ttgcggtgct gaactgattt gcgaatccac ggccataaaa cgttcctgtg atggtgggtg
     3181 cctttcctgt ttcccccggc atcagccttg aaaggggtcc gttgaatagg atacgacaga
     3241 tctgccgggg ctttccaatt agcgaactta tgcaaacaag cggtctctcc ctgaacgcct
     3301 gtgctactaa aagaaataat agaaccggca aaaattaata gaggcgagaa acgtttgccg
     3361 gagctgcttt tgagtgttgc aggggttagc tccgcggggg ctggaaagaa aagacgggcc
     3421 ccatcgccat acttgtggtg cgacgatggg accgcgtcag gtcagggctg ggaagttact
     3481 cccaggtgtg gggacgaatg cacatgcagt cgtagagcag tctctggcag ccgccgcagc
     3541 ggttcagaaa ctgtggattg caggttgtgc aaatgttttc gtacttgagc ccgcctttca
     3601 ggacggtgct gcaagtgggg cagacagggt tcttgtcttt ctggcagtgg cagtcgcctg
     3661 cggcaaccgt agcgcttgtg gcgccggtgc cttcagggct gacattgccg gctttcatcg
     3721 attgattcat ggtttacctc gtttcccgtg tcagggcttg gttttcgaca aaaggctgga
     3781 aaagaggcgc tttccatgaa agcgccttga gagtaccagg gctagtccta cgacttggac
     3841 ttggcgaccg gcttggactt gcccttttca ctaggcttgc cggttgccgg agcggcgtcg
     3901 ctcttgtcgc ccccctgg
//
LOCUS       DAOQNZ010000180         3911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10717_length_3911_cov_3.845695, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000180 DAOQNZ010000000
VERSION     DAOQNZ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10717_length_3911_cov_3.845695"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PKW73_04475"
     CDS             complement(<1..735)
                     /locus_tag="PKW73_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNA72566.1"
                     /translation="MFLTVKLGIQCHNWLMETSAEPENSLKQAEQGSPTTSVHTAVRL
                     ALASSAIFSLMFALIKLLGKAYPAGEILFCRSFFALIPLLPAIIANGGLKVFQTQKPL
                     MHLTRSTMGLVSAFLCIEALKLLPLSEATSFFYSAPLITTVFSIFLLKETISFKKLAA
                     VMLGFGGVLYMLQPHVNHSLSGALIALGSALGAGFVAIELRRMSETEKSITIVSYFMT
                     ACTLVGLLTMPFQFTMPGPTDAAILIG"
     gene            977..1306
                     /locus_tag="PKW73_04480"
     CDS             977..1306
                     /locus_tag="PKW73_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNA72567.1"
                     /translation="MADFDRGRKTGSGTAVLEETRTKPQKPPMFKVLLHNDDYTTMEF
                     VVYVLKNVFYKSPAEAERIMMNVHRQGVGVAGLYPYEIAEAKVDKVTKLARAREFPLR
                     LTIEPEN"
     gene            1370..3859
                     /locus_tag="PKW73_04485"
     CDS             1370..3859
                     /locus_tag="PKW73_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA72568.1"
                     /translation="MLSRELQNTISLASLDAQKRRHEFVTLEHVLLAMTREKTAAEVL
                     TACGADPGYLARQLESYLDQNVERIPEGFSPEEEEFGGEEGLADLKPQITGAFERVLE
                     RAAMQAFSAQRSSIDSGAVLAAMFYERRSHAVYLLEQEGVSRLDVLNFISHGVGKYGA
                     GSARDGVGTDGIDIDAGGLTEPGAEKRKVSALEEFCVDLLEQAKANRIDPLIGREAEV
                     ARTVQVLCRRRKNNPVYVGEPGVGKTAIAEGLALKIARGEVPKALKASHVYALDMGAL
                     VAGARYRGDFEDRLKAVIKELKNLPGSIVFIDEIHTIVRAGAVEGGAMDASNILKPAL
                     ASGELRCIGSTTHNEYKASFEKDKGLARRFQKIDVLEPSISDTIKILQGLKPHYENYH
                     GVHYPDVTLEAAAELSSRHINDRFLPDKAVDVMDEAGALVKLKVEAEQAEVAPDSTAK
                     AEVTPQDVELTVASMAKIPPRTVSGSDKDRLQNLEKELKQVIFGQDHAIETMVKALKL
                     SRAGLGHPDKPIGSFLCSGPTGVGKTELAKQLARIMGVNFLRFDMSEYMEKHTVSRLI
                     GAPPGYVGFDQGGLLTDAVGKTPHCVVLMDEIEKAHPEVFNILLQVMDHGSLTDNNGK
                     KADFRNVILIMSTNAGARDMMGDTIGFQAASGKGSLSEHLAQEAEKAKREREQDKSAS
                     SSSGSYGFGLGKGRGAIERTFSPEFRNRLDAWIPFNPLTFANILLVVDKFVGEVKERL
                     LERSVQITVTPAAREWLAEHGYDRMYGARPMARLIKEKVSEPLAEEVLFGKLEHGGSV
                     LVDVEPAEGGAAEKLKLDLREALAKPETTAE"
BASE COUNT          931 a          983 c         1157 g          840 t
ORIGIN      
        1 gccgatgaga atggcggcat cggtgggtcc cggcatggtg aactgaaagg gcatggtgag
       61 aagaccaacc agggtacagg cggtcataaa ataagaaaca atagtgatgc tcttttccgt
      121 ttcgctcatg cgcctcagct caatggccac gaaaccggca cccagggctg aaccaagtgc
      181 aatgagggcg ccgctcaaac tgtggttgac atgcggttgc agcatataga gcacgccccc
      241 gaaaccaagc atgacggcgg ccagtttctt gaatgagatg gtttctttca gcaggaagat
      301 ggaaaagacc gtggtgatta gcggcgccga gtaaaagaaa ctggtggctt ccgaaagcgg
      361 caaaagtttg agcgcttcaa tgcagagaaa ggccgagacc agacccatgg tggagcgggt
      421 caggtgcatg aggggctttt gggtctgaaa gactttcaag ccgccgttgg caataatggc
      481 cggcaagagc ggaatgaggg caaagaaact gcgacaaaag aggatttcgc cggccggata
      541 ggccttgccc aggagcttta tgagggcaaa catgagggag aagatggcgg aactggccag
      601 ggccaggcgc actgccgtgt gcaccgaggt ggtggggctg ccctgttcgg cttgcttgag
      661 actgttttcc ggttccgcgc tggtttccat gagccaatta tggcactgaa tacccagttt
      721 taccgttaaa aacattaagg agagacgttc ccaccaacgt ctctccttaa tttatgactt
      781 acgggcgttg caccgagctt tgcaatgccg tagtgccttt cgacatagct aatctacaac
      841 agcatctgtg tcgaaataat gacattgtcg tgtcggcgct gtgacaaaag caattttttt
      901 tcagtgcttt tcaagtctgc tcgggtaagc gccccggaga ttgttaaaat agaagcccta
      961 ggggttgagg gaagccatgg ccgattttga tagagggcgc aaaacaggca gcggcacggc
     1021 ggtgctggag gagactcgca ctaagccgca aaagccgccg atgttcaagg tcttgctgca
     1081 caatgacgat tacaccacca tggagttcgt ggtttatgtg ctcaagaacg tcttttacaa
     1141 gtctccggcc gaggctgaac gcatcatgat gaacgtgcac aggcagggcg tcggtgtagc
     1201 cggtctttat ccctacgaaa ttgcggaagc caaggtagac aaggtaacca agctggcccg
     1261 ggccagggaa ttccccctgc gcctgacaat cgagccggag aattaaagct cctgagagtt
     1321 agacttatag attagaacag catggatcat atggaaacgc ggttattaaa tgctcagtcg
     1381 tgaattgcag aatactatct ctcttgccag cctggacgct caaaagcgcc ggcatgagtt
     1441 tgtcactcta gagcatgtgc ttctggctat gaccagggag aagactgctg ccgaggtttt
     1501 gacggcttgc ggggccgacc ccggctatct ggccaggcaa ctggagtctt atctggacca
     1561 gaacgtggag aggattcctg agggcttcag ccccgaggag gaagagtttg gcggcgagga
     1621 gggtctcgca gatttgaagc cccagattac cggcgccttc gaacgagtgc tggagagagc
     1681 ggccatgcag gcattttcgg ctcagcgttc ctccatcgat agtggcgcgg tactggcggc
     1741 catgttttat gaaaggcgct cccatgccgt ctacctgctt gagcaagagg gtgtcagtcg
     1801 tttggacgtg ctcaacttta tttcgcacgg agtcggcaag tacggcgccg gcagtgcccg
     1861 agacggcgtc ggcacggacg gaatcgatat agatgccggc ggacttaccg agccgggcgc
     1921 tgaaaagcgc aaagtcagtg ctcttgagga gttttgtgta gaccttttgg aacaggcgaa
     1981 agccaaccgc atcgaccccc ttattggtcg agaagcggaa gttgcccgca ctgtgcaggt
     2041 gctttgccgg cgacgcaaga ataatcccgt ttatgtgggt gagcccggcg tgggcaagac
     2101 agccatcgcc gaggggttgg ctctcaagat tgctcgcggt gaggtgccga aagcgctcaa
     2161 ggcttctcat gtgtatgccc ttgatatggg tgcccttgtg gccggcgccc gttaccgggg
     2221 cgattttgaa gaccgcctga aagcggtgat caaggaactg aagaacttgc cgggttccat
     2281 tgtctttatc gacgaaattc acaccatcgt ccgggccgga gcggtggaag gcggcgccat
     2341 ggatgcttcc aatatcttga agccggcgct ggcttccgga gaattgcgct gcattggttc
     2401 caccacccac aatgagtaca aggcttcctt cgagaaagat aaaggattgg ctcggcgctt
     2461 tcagaagatt gatgtgcttg agcccagcat ttccgacact atcaagatct tgcagggctt
     2521 gaagccccac tacgaaaact accatggtgt tcattatccg gatgtgaccc tggaagccgc
     2581 cgccgagctt tcctctcggc atattaacga caggttcttg cccgacaagg cagttgatgt
     2641 aatggacgaa gccggtgccc tggtcaagtt gaaggtggaa gccgaacagg cggaggtggc
     2701 gccggattct acggccaagg cggaggtgac tccccaggac gtggaattaa ccgtggcatc
     2761 catggctaaa attccgcctc gcacggtttc cggctccgac aaggatcgcc tgcagaatct
     2821 ggagaaggaa ctgaaacagg ttatcttcgg gcaagaccat gccatcgaaa ccatggtcaa
     2881 ggccctgaag ctctcccgtg ccggtcttgg tcatcccgat aaaccaatcg gctccttcct
     2941 ctgctccgga cctaccggcg tgggcaagac cgaattagcc aagcagttgg ccagaattat
     3001 gggtgtcaat ttcctgcgct tcgatatgag cgagtacatg gaaaagcaca cagtctcgcg
     3061 ccttatcggt gcgcctcccg gctatgtcgg cttcgaccag ggcggcttac tcaccgatgc
     3121 agtcggcaag actccgcact gcgtggtctt gatggacgag atcgagaagg ctcatccgga
     3181 agtcttcaat atcttgttgc aagttatgga tcacggctcc ctcacggaca ataacggtaa
     3241 gaaagccgac tttcgcaatg tcattctcat catgagcacc aatgccggtg cccgtgacat
     3301 gatgggtgat actatcggct tccaggcggc ttcaggcaaa ggcagccttt ccgaacatct
     3361 ggctcaagaa gcggaaaaag caaaaagaga aagggagcag gacaaatccg cttcttcatc
     3421 ttcgggcagc tatggcttcg ggcttggaaa gggacgcggt gccattgagc gcactttctc
     3481 gccggagttc cgtaacaggc tcgatgcctg gattcccttc aatcctctta cttttgccaa
     3541 tattctgctt gtggtagaca aatttgtggg cgaagtgaaa gaacgacttt tggagcgcag
     3601 tgtgcaaatt actgttactc ctgccgccag agagtggctg gcggaacacg gttatgaccg
     3661 tatgtacggc gcgcgcccca tggcccggct tatcaaagag aaagtgagcg agcctctggc
     3721 ggaagaagtg cttttcggca aactggaaca tggcggctcg gtgctggtgg atgtcgaacc
     3781 ggctgaaggc ggcgcggcgg agaaactaaa attggacctg cgggaagccc tggccaagcc
     3841 tgaaaccact gcggaatgat gaaaccatga acgcccaaat gcagcttctc aatcagatta
     3901 atcaaattct c
//
LOCUS       DAOQNZ010000181         3886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10833_length_3886_cov_3.537197, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000181 DAOQNZ010000000
VERSION     DAOQNZ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10833_length_3886_cov_3.537197"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKW73_04490"
     CDS             <1..519
                     /locus_tag="PKW73_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNA72569.1"
                     /translation="MELIRQPIDYLGINWYLRQVVTGKGRVIRVPGAKETLMGWEIHA
                     PALTAMLLNMKSEYRQLPPLYITENGAALKDERTASGVHDLGRMTYTYDHISALEMAS
                     LQGVDIRGYFAWSLMDNLEWSLGYSKTFGLVHVDRETQVRTVKDTGLWYSAVIKANSR
                     GKAASRKGSGRR"
     gene            628..3690
                     /locus_tag="PKW73_04495"
     CDS             628..3690
                     /locus_tag="PKW73_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72570.1"
                     /translation="MQIELEASKCRLVATDGVKASLHQEMHRGQKALRLDFQFSGSGY
                     ALLRIPVNIRLHPNYVFQFQVAGQCPDNTLEFKLLDASTDNVWWVNRPNFKFTTTFQT
                     VVNKKRHLSYAWGPVKEPLQEVAFLDLTITNANGGQGSVIFKGLTYEERPLGSASRQA
                     PEVWASSNRKNAQRVLDEDPYTRWESQGKGELESLIFRFREGQELSALVLEWGEHPVQ
                     DFQIDIKEGNGPWRCTDTIRGATAMRQYLLLPDCDISAVRLTMRKQAAINLPLCAPRQ
                     VSLRSVTFHDRSYAPCHNSLWKRIAKTFPRGYFPRYHQDEQSFWTVVGASGSRQEAII
                     NEEGMLEVGRQTFSLEPFLFNDRRLLTWACGDHNQSLADGYLPIAQVERRHNQTLYQD
                     VRGEDEGPVLKITAFAQGGQEDSTLYARYTIKNESERTIQRGRFYLALRQFQVNPPWQ
                     FLNTPGGFTAIHSLERSGKEVLVNGRLKVVELTPADGFGATTIATDDVVEHLNYGLLP
                     EAAAVYDERGFASGALMWHYEIQPGEEKHFDIAVPYTDRSQVLHLERALEETRSYWLS
                     QLEKVQVEIAAAPDLVRTIKSQLAYILINRDGDAIQPGSRCYRRTWIRDGSLTSAALL
                     QNGFEKEVRAFIEWFAPYQYENGKIPCCVDKRGSDPVPEHDSHGEFIYLVMEYYRFTR
                     DLDFLRALFPRLLKAVAYIQELRSQCLTEDFQAGGNRAHLYGILPPSISHEGYSAHPA
                     YSNWDNLFAMKGLDDAAQAAAILGEKEEAERLRSLAEEFRHCLLASIQASMSLHGIDY
                     IPGANDLGDFDATSTTIGLDPANTLLELPKALEATFERYIEFFRARRDGKPFVDYTPY
                     ELRTVGTFVRLSQADRAHEALDYFMQDRRPAGWNHWAEVVYKNPLTPRFIGDAPHGWV
                     GSDFLRSVRNLFVYETAESLVVGAGIKPQWLEQGVKVVNLPTHFGPLTFSARRQTVDG
                     KTLTTYEVDGPITAPVELHVLVGGKTGASKTLKVTLSQLPCRVQISCP"
BASE COUNT          899 a         1198 c         1037 g          752 t
ORIGIN      
        1 atggaactga tccggcagcc catcgattac ctgggcatca actggtattt gcgccaggtg
       61 gtgaccggca aaggcagggt catccgcgtg ccgggcgcaa aggaaaccct catgggttgg
      121 gaaatccatg ccccggccct gactgccatg ctgctcaaca tgaagagcga ataccggcaa
      181 ctgccgcccc tctacatcac cgaaaacggt gccgccctca aggacgagcg caccgcttcc
      241 ggcgtgcacg atctggggcg catgacctac acctacgacc atatcagcgc cctggaaatg
      301 gcgtccctgc agggagtaga catcaggggt tacttcgcct ggagcttgat ggacaatctg
      361 gagtggtccc tgggctacag caaaaccttt ggtctcgtcc atgtggacag agaaactcag
      421 gtgcgcacgg tcaaggatac aggactctgg tactcagccg tcatcaaagc aaactcccgg
      481 ggcaaagccg ccagccgcaa aggtagtggt cggcgctgaa cagtttttcc tcagccagtt
      541 tttcatcagc cggttcctca gccagcaagt gcgcgcaggc aggtcgccac cagcctgcac
      601 gcctccaacc gtagagatca aaagcacatg caaatagaac tagaggcaag caaatgtcgt
      661 ctcgtggcca cggacggtgt caaagccagc ctgcatcagg agatgcaccg gggtcaaaag
      721 gctctcaggc tggactttca gttttccggc tcgggctatg ccctgctgcg cattccggtc
      781 aacatcagac tgcaccccaa ctacgtcttt caattccagg tggccggtca gtgtccggac
      841 aacaccctgg agttcaagct cttggatgca tccaccgaca atgtctggtg ggtgaacaga
      901 cccaatttca agttcactac gacctttcag accgtcgtca acaagaagcg ccacctgagc
      961 tatgcctggg gtccggtgaa agagccgctg caagaagtcg cttttctgga cctcactatc
     1021 accaatgcca acggcggtca gggctcggtt atcttcaagg gtctcaccta tgaggagcgg
     1081 cccctcggct cggcatccag gcaggctcct gaggtctggg cttcttccaa tcgcaaaaat
     1141 gctcagcgcg tcctcgacga agatccttac accagatggg aaagccaagg caaaggcgag
     1201 ctggaaagcc tcattttccg cttccgggaa gggcaagaac tttcagctct ggtgcttgaa
     1261 tggggtgaac atcccgtgca ggacttccag atagacataa aggaaggcaa cggtccctgg
     1321 cgctgtaccg ataccatccg cggcgccact gccatgcgcc agtacctctt actccccgac
     1381 tgcgatataa gtgccgtccg gctcaccatg cgcaagcagg ccgcaatcaa tctgcccctc
     1441 tgcgcaccca ggcaggtatc gctcaggtca gtcactttcc atgatcgcag ctatgcacct
     1501 tgccataatt ccctctggaa gcgcatagca aagacctttc cgcgcggcta ttttccccgc
     1561 taccatcagg acgaacagtc tttctggacc gtggtgggtg cctcaggcag ccgccaggaa
     1621 gcgatcatca atgaagaggg catgctggaa gtcggccgcc agaccttctc tctcgaaccc
     1681 ttcctcttca acgacagacg cctgctcacc tgggcttgcg gtgatcacaa ccagagtctg
     1741 gcggacggct acctgcccat cgctcaagta gagcggcggc acaaccagac tctctatcag
     1801 gacgtgagag gcgaagatga aggccctgta ctcaagataa ccgcctttgc ccagggcggt
     1861 caggaagact ccactctcta cgctcgctac accatcaaaa acgagagcga gagaaccatc
     1921 cagcgcgggc gcttctatct tgctctgagg caattccagg tcaatccgcc ctggcaattc
     1981 ctcaatactc cgggcggctt cacagccata cacagcctgg aaagaagcgg caaggaagtg
     2041 ctggtaaacg gccggctcaa ggtagtggag ttaaccccgg ccgacggttt cggagccact
     2101 accatcgcca ccgacgatgt ggtagaacac ctcaactacg ggctcttgcc ggaggccgcc
     2161 gccgtctatg acgagcgcgg attcgcctcc ggcgcactca tgtggcacta tgaaatccag
     2221 cccggcgaag agaaacactt cgatattgca gtaccataca ccgaccgttc acaggtcttg
     2281 cacctggaaa gagcccttga ggaaacccgc agttactggc ttagccaact tgaaaaggta
     2341 caggtggaga ttgccgctgc accggatctg gtgcggacca tcaaaagcca gctagcctat
     2401 atcctcatca accgagacgg cgatgccatc caacccggct cccgctgcta ccgccgcacc
     2461 tggattcgag acggctcact gaccagcgcc gccctcttgc agaacggctt tgaaaaagaa
     2521 gtgcgcgctt tcatcgaatg gttcgcgccc tatcaatacg aaaacggcaa gatcccatgc
     2581 tgcgtggaca aacgaggctc agacccggta cccgaacatg acagccatgg agagttcatc
     2641 tacctggtca tggagtacta ccgcttcacc agagacctgg actttctgcg cgctcttttc
     2701 ccgcgccttc tcaaagccgt ggcctacatt caggagttgc gcagtcagtg cctgacggaa
     2761 gacttccagg cggggggcaa ccgtgcacac ttgtacggta tcctgccgcc ttccataagt
     2821 catgaaggat attcggctca tccggcctat tccaactggg acaatctctt cgccatgaaa
     2881 ggcctggacg atgccgccca ggccgccgcc atcctgggtg agaaagagga agccgaacga
     2941 ctgcgttctc tggctgagga gttccggcac tgcctgctgg cttccattca ggcttccatg
     3001 agcttgcatg gcatcgacta cattccaggc gccaatgacc tgggcgactt tgatgccact
     3061 tccaccacca tcggtcttga tccggctaat accttgctgg agctgccgaa ggctctggaa
     3121 gccactttcg agcgctacat cgaattcttc cgggcccgcc gcgacggcaa accttttgtg
     3181 gactacacac cctacgaact gcgcaccgtc ggcacctttg tgcggcttag ccaggctgac
     3241 cgagcccatg aagcactgga ttacttcatg caagatcgcc gcccggccgg ttggaatcac
     3301 tgggcggaag tggtctacaa aaacccgctc acaccgcgct tcatcggtga cgcaccccat
     3361 ggctgggtgg gctccgactt cttgcgttcg gtgcgcaatc tctttgtcta cgaaaccgcc
     3421 gaaagcctgg tggttggtgc cggtatcaaa ccccagtggc ttgagcaggg cgtcaaggtg
     3481 gtcaacctgc ccacccactt cggaccgctc accttcagcg cccggcggca aaccgtagac
     3541 ggcaagaccc tgaccaccta cgaggtggac ggaccgatca cggcaccggt ggagctgcac
     3601 gttctggtgg gaggcaaaac aggggcaagc aaaactctga aagtcactct cagtcagctg
     3661 ccctgccgcg tgcagatctc ctgtccctaa gttttgacga tagttttgga gagagttggc
     3721 cctgaggcca actctctttt tgacccaatg cagccgaccg gtctactatc tttcggcaaa
     3781 acaaaccagg ccggcaattc tggaaatcga actcaaggaa ctcaaagagc gagaaggagc
     3841 aagaaggaac aagaagtagc aagaataagc aacagaccct tcaatg
//
LOCUS       DAOQNZ010000182         3886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10834_length_3886_cov_3.382407, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000182 DAOQNZ010000000
VERSION     DAOQNZ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10834_length_3886_cov_3.382407"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKW73_04500"
     CDS             complement(<1..272)
                     /locus_tag="PKW73_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72571.1"
                     /translation="MNTPVLIQGGMGVGVSNWRLAKAVSMEGQLGVVSGTMLDTVFAR
                     RLQDGDPEGALKRACEAFPLPEIADRLWNHFFIPAGKADEAPYRPVP"
     gene            660..1109
                     /locus_tag="PKW73_04505"
     CDS             660..1109
                     /locus_tag="PKW73_04505"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNA72572.1"
                     /translation="MKEAESTTKAKGTATKRAGTKKTTVESAAVETQVSPKEAVAVET
                     QVSPKAAAATETQVSPKEAAAPKKKAAAPAVSVLEVVPAVCEQPSENPKQACEQPAAS
                     ESAKEVCFEQVAKRAYELWLSKDCQHGTDVENWLEAEIQVKAEATKK"
     gene            1695..2426
                     /locus_tag="PKW73_04510"
     CDS             1695..2426
                     /locus_tag="PKW73_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72573.1"
                     /translation="MHKLVFSAPLFAFLIASFIWPAAWAQVASELAILSSWKANAKTS
                     HLGNQLLTLLDATNRAIAVNPNDKGALYRRGYLYSTVGCTQSAISDLSRVVQIDPYFA
                     AAYTERGICYLDGKNYDRSLSDLNRALQLNPYSGDAVFARGRLWLAQGKAQLALSDFR
                     YCQQSNVKFAPVLPGEFPANQYDAPNYYIGCCYEAMNKPDEAVKFYKASLKTQPLGSV
                     GYLHRYADQPLDTKYRVSSLEHYGY"
     gene            2457..2729
                     /locus_tag="PKW73_04515"
     CDS             2457..2729
                     /locus_tag="PKW73_04515"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNA72574.1"
                     /translation="MTDSLLAGALSQCSGDRLRQLAVSDTGFVFDPQTGQSFTVNATG
                     RLVLDCLKRSDGLEEAAQCLAEDCDVPLDLALSSVEAFLMQLGRYL"
     gene            2726..3808
                     /locus_tag="PKW73_04520"
     CDS             2726..3808
                     /locus_tag="PKW73_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNA72575.1"
                     /translation="MRNWRHWKVAVTGINAHAENPGPGLAVARCLVEHREFRGSVLGL
                     AYDALDAGLYDHENCQSSYLIPYPSAGDEALLERIKVIHALEGIDAVIPCLDSELQNF
                     ISLRSELAGLGIKMLIPSRDQFNLRAKNHLVDLCSKIGVAVPRSKMLTDPGFFDRVAR
                     EGFEYPLVVKGVFYDAAIVYNAFEAKAAFSRLVGKWGYPCLVQEVVNGDELNLAGVGD
                     GQGNLSGYVMMRKRAVTDKGKAWAGVTVVDEALHATASKLVRALAWRGPLEVEVIKGT
                     DGKIYLIEINPRFPSWIYLSHAVDRNLPVAVLRMLADEKDVQLTQHEPGTFFIRHARE
                     LIVNLKDFESVLVNGYCGGDVVDRSA"
BASE COUNT          875 a          994 c         1036 g          981 t
ORIGIN      
        1 ggaaccggcc tgtacggggc ttcatctgcc ttgcccgccg gaataaagaa gtgattccat
       61 agccggtctg caatttcggg tagaggaaag gcctcacaag ccctctttag tgctccttcc
      121 gggtcaccgt cctgaagtcg gcgagcaaag accgtgtcga gcatggtacc ggatacgaca
      181 ccaagctgcc cttccattga tactgctttg gccagccgcc agttggaaac gccgacgccc
      241 atgccacctt gaattagtac cggagtattc actgctcttt cctctcgccg attttgcttc
      301 caagtccgga ttgttctccg gcggcgagtg tatcaatgac aggccggtga aagtagtggc
      361 cacctatttg tgcccgtgcc gaccgagctt gccggggctc ctttcagggc tttgtaaacc
      421 tggtgccaac ccgctcaggt caagactttc ctttggtaag cgttgtttta cgtgacatag
      481 tgtgtcgaaa ggtttctatc cggccgtttg tatatgcaac agccgtgtgc cgccgccggt
      541 aaactatccg gcgaattttg gggttttggg aggtgatatt gttttatcgg tagcccttcg
      601 agtgcatacc tttagcgagt aaatagccga cggatggttt ggctagtggg agtgtggaaa
      661 tgaaagaagc tgaatcgaca actaaagcaa aaggcaccgc tactaagcgc gccggtacca
      721 agaaaaccac agttgagtct gctgccgtag agactcaggt atctcctaaa gaagctgttg
      781 ccgtagagac tcaggtatcc cctaaagcgg ctgctgccac tgagacccag gtatctccaa
      841 aagaagctgc cgcccctaag aaaaaggctg ctgcaccggc tgtatctgta ttagaggtag
      901 tgcctgctgt ttgtgagcag ccttccgaaa atcccaagca agcttgtgaa cagcccgctg
      961 cttccgaaag cgccaaagaa gtatgtttcg aacaagtggc caaacgcgct tatgagctct
     1021 ggttgagtaa agattgccag cacggcaccg atgttgaaaa ctggctggaa gcggaaattc
     1081 aggtaaaggc cgaagctact aagaaataga tttgggccga tatcgggcac tattcatgtc
     1141 ggtcagatat tagtcaacag cgctgccggg acgaggtggc taagatctgg tatcaagaag
     1201 ttgtagtttg gtgctgtttt ttgtctgacg atttctacaa aaaccttctt tcaaaagagg
     1261 caccaggggt ctctcggaaa aacacgagca aatgtaacaa tagtgaaatt gcgaaatatt
     1321 acatcacggt tttgggactt tttgctaatt gaaacccttg ctgtaactgc attcccgccg
     1381 tcaagcagaa ttaattttgg ctgttacatg agtttagggt tttcgccctt tgttttttaa
     1441 agacatgggt tcggtctgct tttgtcctct cactcggtgc cttaactcgg tcgttccagc
     1501 tcggtcggcc caaagtagtc ggttaaatcg gtcgccccag gcagcccccc taggcagccc
     1561 ccccccaatc agtcaatcca aatcagcccc tccatttttt ctccacaatt tctccattcc
     1621 ttttccacag gctctctcta gcatgatttg tgtagcgcct cccggcatgc cgcctcattt
     1681 agaggagagt caaaatgcac aaattagttt tctcggcacc gcttttcgcc ttccttattg
     1741 cctcgttcat atggcctgcc gcctgggctc aggttgcctc tgaacttgcc attctcagta
     1801 gttggaaagc caatgccaaa accagtcatc taggcaatca gcttctcact ttgctcgatg
     1861 ctactaaccg ggcaattgcc gtcaatccca acgacaaggg tgccctctac cggcgcggct
     1921 atctttacag cacggtgggt tgcactcagt ccgccatcag cgatcttagc cgggtggtgc
     1981 aaattgatcc ctattttgct gctgcttata cggagcgcgg catctgctac ctggacggca
     2041 aaaactacga ccgctccctc tccgatttga accgggcctt gcagttaaat ccttattccg
     2101 gcgatgccgt ttttgccagg ggccggctct ggttggctca gggtaaggcg cagttggccc
     2161 tcagtgactt tcgttattgc cagcaaagca atgtcaaatt tgctccggta ttgccggggg
     2221 agtttccggc taatcaatat gatgcgccca actattacat aggttgctgc tatgaagcca
     2281 tgaacaaacc ggatgaagcg gtgaagttct acaaggcttc cctgaaaacc cagcccctgg
     2341 gttcggtcgg ctatctccat cgctacgccg atcagcccct cgataccaag tatcgggtga
     2401 gcagccttga gcactacggt tactgaacta cattgaaagt tgagaggaat ttgcatatga
     2461 ccgatagtct actggccgga gccctttccc agtgcagcgg cgacaggctc aggcaactgg
     2521 ccgtcagcga tacgggcttt gtctttgatc cgcagaccgg acaaagcttc accgtcaatg
     2581 ccaccgggcg attggttcta gattgtctca aacgcagtga cggtcttgag gaagcggctc
     2641 agtgtctggc tgaggattgt gacgtgcctc tggatttggc tttaagtagc gtggaggctt
     2701 tcttgatgca attggggagg tatctatgag aaactggcgg cactggaaag ttgctgttac
     2761 aggcatcaat gctcatgctg agaaccctgg tccgggcctg gccgtggccc gctgtctggt
     2821 tgaacaccgg gaatttcgcg gctcggtgct cggtttggct tacgacgccc ttgatgccgg
     2881 tttgtatgac cacgagaatt gtcagagcag ctacttgata ccttatcctt ccgccggcga
     2941 tgaagcctta ttggagcgaa tcaaggtaat tcacgctctg gaaggaattg atgccgtcat
     3001 tccctgcctc gattcggaat tgcagaattt catcagcttg cgcagcgaac ttgccggtct
     3061 gggaatcaaa atgctcattc ccagtcgaga tcagttcaat ttacgggcca agaatcatct
     3121 tgtggatctc tgctccaaaa tcggtgtagc cgtgcccagg agcaaaatgc tcaccgatcc
     3181 aggctttttt gatcgcgtgg ccagggaagg ctttgagtat cccctggtgg tcaaaggcgt
     3241 tttctatgat gccgctattg tttacaacgc cttcgaggct aaagcggctt tcagccgcct
     3301 ggttggtaag tggggctatc cctgtctggt gcaggaagtg gtcaatggcg atgagctaaa
     3361 tctggccgga gtcggtgacg gacagggcaa cttgagcggt tatgtgatga tgcgcaagcg
     3421 cgccgtcacc gataaaggca aagcctgggc cggggtgacc gttgtggatg aggccctgca
     3481 tgccactgca tccaaattgg tacgggcgct agcctggcgc ggaccgctgg aagtggaagt
     3541 aatcaaaggg acggacggca agatctattt gatagagatc aatccgcgct ttccttcctg
     3601 gatttatttg agtcatgccg tggatagaaa cttgcctgtg gcagtgctgc gcatgctcgc
     3661 cgacgagaaa gatgtgcaac tgactcagca cgagcccggc acttttttca ttcgccatgc
     3721 ccgggaacta atagtcaact tgaaagactt cgaatcggtg ctggtgaatg gctattgcgg
     3781 cggcgacgta gtcgatcgaa gcgcctgatt ttccttgaga caaaattatt tggagcaaaa
     3841 cctatgcctg agaatagcag tccgaaacga ccaaagtttg cctggg
//
LOCUS       DAOQNZ010000183         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10876_length_3878_cov_3.897463, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000183 DAOQNZ010000000
VERSION     DAOQNZ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10876_length_3878_cov_3.897463"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKW73_04525"
     CDS             <1..428
                     /locus_tag="PKW73_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNA72576.1"
                     /translation="KTFIFYRDDSQTKSRLKTFAQVDGDLAEFVGVEGGTDNALDLRH
                     CLELLGGRGLRRLLCEGGGRLAGSLLAADLVDEVHWFVAPKLLCDASAPLAVASPLER
                     PLSAVLQLEEMSVTEVLPDFLLHGWMPGRSAFLSGRTET"
     gene            518..1867
                     /gene="miaB"
                     /locus_tag="PKW73_04530"
     CDS             518..1867
                     /gene="miaB"
                     /locus_tag="PKW73_04530"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNA72577.1"
                     /translation="MTATTAEKKKVFVETFGCQMNKSDSEHMLGLLDEIGYEPTPDIK
                     GADLMILNTCAIREGAEDKVYSYLGAWRGYKEARPGSLIAVGGCVAQDAGEAMRKRAP
                     YVDLVFGTHNLHRLPELVMKASSGEPVVEIYQELPEDLPETPVHRLNDISAWVSIIYG
                     CDYNCTYCIVPYVRGREKSRSPQSIFEEVKSLAESGYKEVTLLGQNVTAYGHDLEPKL
                     HLGDLLRQLAVPELEAIKRLRFVTGHPRDLNVEIIDAVRDLPQACEYFHIPIQAGDDR
                     TLRRMARGYNVDFYRRKVDEIRSRIPDAAITTDLIVGFPGESEEEFMNTVRLVEEICF
                     DVANTAAYSPRQHTPSANWDGQVPEAEKYERLRYLNTVVGDVSHRLNKRLLGRKVEIL
                     VEGRSDRGMNRLMGRTRTNKIVNFEGPDALIGQLVDVEVTAANPWALRGALKVGLTV"
     gene            1957..2991
                     /locus_tag="PKW73_04535"
     CDS             1957..2991
                     /locus_tag="PKW73_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72578.1"
                     /translation="MKGESFLKQGDFQRALTKAETAYRNAKITRLEYLKLKGAVYNGL
                     ENNTALAELCRDALTLYPKEGEFYYLRACTYKDTQNPKEALADLNKAIALSPNSAKAY
                     LKRARILFVLTENLSGGDYLKQARADLKRSIELDGTQAEAFDYQGIWLSTEGKYQEAI
                     SSFSKAISLNNNDYRYFSHRAGAYRTVNELEKALSDMDRLVVLRPNVSQGWITRAETL
                     EQMKRYDDALASYERAVKLDQNKGDSRKARARLNLRIGKYKEAIVALDFLVKLSPADD
                     EHYWNRGLAWQGLKQYDRALKDFNIAIDLNPQLPSYYESRAKLNNLRGEKNQAAQDKR
                     RADKLRQERI"
     gene            complement(3074..>3878)
                     /locus_tag="PKW73_04540"
     CDS             complement(3074..>3878)
                     /locus_tag="PKW73_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72579.1"
                     /translation="EAGAAGVHFEDQLASEKKCGHLGGKVLVPTSTFIRTLSAARLAA
                     DVLGVPTIITARTDALDASLITSDICDIDKPFIVGERTPEGYFRVKQGIEPVIARGLA
                     YAPYADVLWFESSKPDLEEARVFAEAIHARFPGKLLAYNCSPSFNWKQHLDDKTIRSF
                     NEELGKMGFKYQFITLAGWHATNLSAFQLAKDYAKEGMAAYVRLQEEEFASQSEGYTA
                     VRHQREVGTGYFDKVLVTASGGEASTQALKGSTEEDQFQQPVKQLVSVN"
BASE COUNT          867 a          958 c         1090 g          963 t
ORIGIN      
        1 gcaagacttt tatcttttac agagatgact ctcaaacaaa gagccggctg aaaaccttcg
       61 cccaagtgga cggagatctt gctgagtttg tgggcgtgga gggtggtaca gataacgccc
      121 tcgacttgcg ccattgtctt gaacttctgg gcgggcgcgg cttgcgccgc ctcctttgtg
      181 aaggcggcgg ccggctggcc ggcagtttgc ttgccgcgga tctggttgat gaagtgcact
      241 ggtttgtggc ccccaagttg ctttgcgatg cttcggcacc cctggcggtg gcatcaccgc
      301 ttgagcgacc gctttcggcg gttttgcaat tggaagagat gtctgtgacc gaggtgcttc
      361 ctgactttct tttacatggt tggatgcccg gccggagcgc ttttctctcc ggtcggactg
      421 agacttaggc ctgtctaacc ttagaagcgc ctgactactg ttatgattcc agtgtctata
      481 tttaagctat gcaatatgtt tatcgggtgt cagtttgatg acggctacca ctgctgaaaa
      541 gaaaaaggtc tttgttgaaa ccttcgggtg tcagatgaat aaatccgact ccgaacacat
      601 gctggggctg cttgatgaga ttggttatga gccgaccccg gatatcaaag gcgcagatct
      661 gatgatcttg aatacttgtg ctattcgcga gggtgcggaa gacaaggttt acagttatct
      721 tggcgcctgg cgcggttaca aggaagcgag accgggttcg ctcattgccg tgggtggttg
      781 cgtggcacag gatgccgggg aagccatgcg caagcgcgct ccatacgtgg atcttgtttt
      841 cggaacccac aatctgcacc gcctgccgga acttgtcatg aaagcttcca gcggcgagcc
      901 ggtggtggaa atttatcaag aattgcctga agacttgccc gagacgccgg tgcatcgtct
      961 caatgacata agcgcctggg tttccattat ttatggctgc gattataact gcacctattg
     1021 catcgttcct tatgtgcgtg gtcgcgaaaa gagccgttca ccccagtcta tttttgaaga
     1081 agtgaagagc ctggctgaaa gcggttacaa ggaagtgacc ctccttggtc agaacgtaac
     1141 tgcctatggt cacgatttgg agccgaagct tcaccttggt gatttgcttc ggcaattggc
     1201 cgtaccggaa cttgaggcca tcaaacgctt gcgttttgtt accggtcatc cccgcgatct
     1261 aaatgtagaa attatcgatg ccgtcagaga tttgcctcag gcctgcgagt atttccatat
     1321 tcctattcaa gccggtgacg atcgtacttt gcgccgtatg gccaggggct acaacgttga
     1381 tttctatcgt cgtaaagtcg atgaaattcg ctcgcgcata cctgatgccg ccatcaccac
     1441 cgatctgatc gtcgggtttc ccggtgagag cgaagaagag tttatgaaca cggtgcgcct
     1501 ggtggaagag atttgctttg acgtggccaa tactgccgct tattctcccc ggcagcacac
     1561 cccctccgcc aactgggatg gacaggtgcc ggaggctgaa aagtacgaac ggctgcgtta
     1621 tctcaacacg gttgtgggcg atgtttccca ccgtctcaac aagagattgc tcgggcggaa
     1681 ggtggaaatt ctcgtagaag gccgttccga ccgcggtatg aaccggctca tgggccgcac
     1741 ccgcaccaac aaaattgtca atttcgaagg accggatgct ttgatcggtc agcttgtgga
     1801 tgtagaggtg acggccgcta atccatgggc tttgcgcggc gcccttaagg ttggtttgac
     1861 tgtttgaagt accgaagttt caagcgaatt ttcgcttgcc tttgtttgct gttttcgatt
     1921 tggcctgctc aggccggcga acccagccct gcttttttga aaggggagag ttttctcaag
     1981 caaggtgatt ttcagcgcgc actaacaaaa gccgagaccg cctatcgcaa cgcaaaaatc
     2041 accaggctgg agtatctcaa attaaaaggg gcggtctaca acggcttaga gaacaacacg
     2101 gcgctggcag agctatgccg ggatgcgctc actctctatc cgaaggaagg cgagttttat
     2161 tatctgcgag cctgtaccta caaggatact caaaatccta aagaggcact ggctgacttg
     2221 aacaaagcca tagctctcag tccgaattcg gcaaaagcct atctgaagag agcgcgtatt
     2281 ctgtttgtgt tgaccgagaa tcttagcggt ggcgattatc tgaaacaggc gcgagccgat
     2341 ctcaaacgat caatcgaact ggatggtacc caggctgaag ctttcgatta tcagggcatc
     2401 tggctatcca cagagggcaa gtatcaggag gctataagtt ccttcagcaa agccatttct
     2461 ttgaacaata acgactatcg ttatttctct cacagagccg gtgcctatcg caccgtcaac
     2521 gaattagaaa aggcgctctc tgacatggat cgccttgtgg tcttgcgtcc taacgttagc
     2581 cagggctgga ttacccgggc tgaaaccctt gagcagatga agcgctatga cgatgccctt
     2641 gcttcttatg agcgggcagt taagttggac caaaataagg gagacagtcg aaaagcgcga
     2701 gcgcggctaa atttgcggat tggaaaatac aaagaggcaa tcgtcgccct cgatttcctg
     2761 gtcaaattgt cgcccgccga tgacgaacac tattggaacc gaggtctggc ctggcagggt
     2821 ctcaaacagt atgaccgtgc cctcaaggac ttcaacattg ctatagatct taatccgcaa
     2881 ctgccctctt actacgagtc gagagcaaaa ctgaataacc tgcgtgggga gaagaatcag
     2941 gcggcgcagg ataagcgcag ggccgataag ttgcgccagg aaagaatcta ggtcaacctt
     3001 attctaatcc aaatgaaaaa cagcccctgg tgcgagggct gttttccatt tttggctggg
     3061 gaaaatttgg tcgctagtta accgacacca gttgtttgac cggttgctgg aactggtctt
     3121 cttccgtgga gcctttcagg gcctgggtgg atgcttctcc gccactggct gtgaccagta
     3181 ctttatcaaa gtagccggtg ccaacttctc tctggtgccg cacggcggtg tagccctcac
     3241 tctggctggc gaattcctct tcttgcagtc ttacataggc agccatgcct tccttggcat
     3301 agtctttagc caattggaag gcactgagat tggtggcatg ccaaccggcc agggtgatga
     3361 actgatattt gaaacccatt ttgcccagtt cttcattgaa tgagcggatg gttttatcgt
     3421 ccaggtgctg cttccagttg aaggatggtg agcagttgta ggctagcaac ttgccgggga
     3481 agcgggcatg aatggcctcg gcgaagacgc gggcttcttc cagatctggt ttggagcttt
     3541 cgaaccagag cacatcggca tagggagcgt aggccagccc tctggcgata accggctcga
     3601 tgccttgttt gacgcggaag tagccttccg gggtgcgttc tcccacgatg aagggtttgt
     3661 caatgtcgca gatgtcggaa gtaatgagac tggcatccag ggcatcggtg cgggctgtga
     3721 tgatggtcgg cacgcccagc acatcggccg ccaggcgggc ggcgctcaag gtgcggatga
     3781 aagtagaggt tggtacgagt accttgcctc ccaggtgacc gcatttcttt tcagaggcca
     3841 actggtcttc gaagtgtact ccggcggctc ctgcttca
//
LOCUS       DAOQNZ010000184         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10911_length_3872_cov_4.728583, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000184 DAOQNZ010000000
VERSION     DAOQNZ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10911_length_3872_cov_4.728583"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..2079
                     /locus_tag="PKW73_04545"
     CDS             439..2079
                     /locus_tag="PKW73_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HNA72580.1"
                     /translation="MEELGVLTLSESDWAEAKHRASVIGPLAELSTVGRVAAEEAAAK
                     LGISTRMVYALVKRCRASNGLVTDLASGCSSGGKGKQRTTPEVNCIIREVIQTHYLSR
                     QKKSAAVIIREVRMRCRQAGYKLPAGNTIRARLRSLDPLVVAQKREGFEATRNLRPAA
                     GKAPEPEKPLDIVQMDHTPVDVIVVDDGSRESIGRPYLTLAIDTLTRCIVGMLLSLEA
                     PSATSVGLCLAHTVSDKRAWLDRLSLTDISWSMSGKPVLVYTDNAKEFKSEALKRGCE
                     QHGINLDYRPGGQPHFGGIIERVIGTAMKMAHELPGTTFSNIQERGKYDSEARAIFTL
                     SELEQWLALAICTYHESIHNGLWESPSACWKRHTETVKPKTVKNERAFLIDFLPVIRR
                     SISRTGFVIDHIGYYADVLKPWIARRENLEKFIIRRDPRDLSRIWVLEPGSNQYLELP
                     YRSISNPAVTLWEHKKAVEKLREKGRAQINELELFRMITLMRKIEESASKETKKARRD
                     KMRRSHLEDPAKPTLQAPPDRPEREVCQVNPFSDIEEW"
     gene            2084..2992
                     /locus_tag="PKW73_04550"
     CDS             2084..2992
                     /locus_tag="PKW73_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HNA72581.1"
                     /translation="MGGKTIKYLTPAAQAATLLPDEERIQHLRADRWIGYTKARSALQ
                     RLEELFNWPSKLRMPNLLIIGPTNNGKSMIIEKFKRDHLWTAVREQADHEVIPVVVVQ
                     TPSEPAVARFYAMLLASIGAPIRPKARIAELEQLTLRLLKSVQAKILVIDELHNLLAG
                     TTDVRQEFLNLLRFLGNELRIPIVGVGTRDAYLAIRSDAQLENRFEPLLLPVWEEGDE
                     LLALLASFSAVLPLKRVSDIANEATARYILARTEGTIGEIAKLLTAAAIVAIETGEES
                     VNQRTLGQAAYLSPTERRQTFERALL"
     gene            3067..>3872
                     /locus_tag="PKW73_04555"
     CDS             3067..>3872
                     /locus_tag="PKW73_04555"
                     /inference="COORDINATES: protein motif:HMM:NF018259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HNA72582.1"
                     /translation="MVYGLSLKDLIEHDLGFCRRQQQRHWLDMAPPAKLLTAVAARTG
                     IPLERIQQSTIAALRPVLYAYFDRKFSACSTVLMAQPKAPLHLNLQKWILRHELVNEF
                     TACRRCLDNYPNAAVLLPWRLRIITSCPEHGVMLEAVRITGDTVEWRKDAEELAPALV
                     SRLDLRTWQALTTGSVNLTGGSVPAGLWFRLLRTIWDELNRPLEECHDKAVLAVWTGI
                     TRLPLVTKDFWHLRSDRKHAICVGTAIDMLETGAAVPEGIDGRYFAGSRRG"
BASE COUNT         1049 a          932 c         1050 g          841 t
ORIGIN      
        1 aactccagac cgtagtcgtg tacacgagca cttaccatat tgccctcctt tgcgttgata
       61 aggcaacgac gctgcctccc ggagggcgac cggctgggct ctgccgtcaa ggcggcaaga
      121 gctaactaca aggacaaaag aaaaaccgaa cggcttgacg gtggtgggcg ctcagccggt
      181 aaacttgaag aacagcggag agagccgcga agtcaggctc acaacgctta atcgatcgga
      241 aaatgagtga aatctgatgc ggcatcgctg tcagattcag aagtcgactt cacaacggaa
      301 gtgaatatac gggctttgtg caagcgcctt ttaatacatg acaatggact ttctttcaga
      361 agacctcttc actgttaagc ttttagtcag aagtgctgtt cactggatgc accagaaccc
      421 acgatttaaa gaggacatat ggaagagctg ggggttctaa ccctatctga gagtgattgg
      481 gctgaggcaa aacatcgagc ctcggtaatt ggtccgctag ccgaattgtc taccgtgggc
      541 agagttgcag cagaggaagc cgcggcaaaa ctaggcatct ccacccgaat ggtctatgcc
      601 ctggtaaaac gttgccgggc aagcaatgga ctggtgacgg atcttgcatc cgggtgttca
      661 agtggcggca agggaaaaca gcgaacgact ccggaagtca actgcattat cagggaagta
      721 attcaaacgc attacctgtc aaggcagaaa aaatctgcag ctgtgattat cagggaggtc
      781 agaatgcgtt gtcggcaagc tgggtataag cttccggcag ggaataccat acgggctcga
      841 cttcgttcgc tcgatccact agtagttgcg caaaagaggg aaggtttcga ggcaacaaga
      901 aaccttagac cggcagcagg aaaggcgcca gagccagaaa agccgcttga tatcgttcag
      961 atggatcaca ccccggtgga tgtgatagta gttgacgatg gttcccgaga atcaattggt
     1021 cggccatacc tcactctggc tatcgacacc ttgactcgat gcatagtagg catgctgctt
     1081 agcttggaag ccccgtcagc cacatctgtt ggtctatgcc ttgcccacac tgtcagcgac
     1141 aaaagagctt ggctagatcg gcttagccta actgatattt catggtcaat gagtggaaaa
     1201 ccggtgctgg tctacaccga caacgccaaa gaatttaaga gcgaagcact caagcgaggc
     1261 tgcgagcaac acggaattaa tctcgattac agacctggag ggcaaccgca ttttggcggc
     1321 atcatcgaac gcgtcatcgg aacggcaatg aagatggctc atgagttgcc tggaacaaca
     1381 ttttcaaaca tacaagagcg cggcaaatac gattcagaag cacgagcaat ttttacactc
     1441 tcagagcttg agcagtggct agcgctggca atatgtacct atcacgaaag tattcataac
     1501 ggactttggg agagtccatc ggcctgctgg aagagacata cagaaacggt caagccgaaa
     1561 acagtcaaaa acgagagagc ctttctaata gattttctcc ctgtaattcg gcgctctata
     1621 agtcgcacag gctttgtgat tgatcacatc ggttattatg ccgatgtcct taaaccatgg
     1681 atagcgcggc gagagaatct ggagaagttc ataattcgcc gcgatccccg agacctcagc
     1741 cggatctggg ttttggaacc aggaagcaat cagtacctgg agctgccata ccggtcgatt
     1801 tcgaacccgg ctgttaccct atgggagcac aaaaaggcag ttgagaagct ccgggagaag
     1861 ggccgagcac agataaacga attggaactg tttcgcatga tcacgttgat gcgaaaaatc
     1921 gaggagagtg cctctaaaga gacaaaaaaa gcacgacggg acaaaatgcg gagaagtcat
     1981 ttggaagatc cggcgaaacc gactctgcag gcaccgccag atcggccaga gagagaggtt
     2041 tgccaagtca acccattcag cgacatagag gaatggtgag ctgatgggtg gtaagacaat
     2101 taaatactta actccggcag cccaagctgc aacgctgcta cccgacgaag agcggataca
     2161 gcatttacgc gccgatcgct ggattggcta cacaaaagca cggtctgcgc tgcagcgcct
     2221 tgaagaattg tttaactggc caagcaagct cagaatgccg aaccttctga ttattggtcc
     2281 caccaataat ggaaagtcca tgatcataga gaagttcaaa agagaccatc tatggacagc
     2341 cgtgcgcgaa caagcagacc acgaggtgat tccagtggtg gtggtacaga caccatcaga
     2401 gcccgctgtt gcaaggttct atgcaatgct tctggccagc ataggtgccc caattcgtcc
     2461 gaaagccaga attgctgaat tggagcagtt gacccttagg ctgctcaaaa gtgtgcaagc
     2521 caaaatatta gtgattgatg aactgcacaa tttactggca ggcacaacag acgtgcggca
     2581 agagtttctc aatttgcttc gattcttagg caacgagctt cggataccaa tagtcggcgt
     2641 cggcaccaga gatgcttatt tggcaattcg gtctgatgct cagctggaga acagatttga
     2701 gccgctcctc ttgcctgttt gggaagaagg cgatgagcta cttgccttgc ttgctagctt
     2761 cagtgcagtg ttgccactta agcgagtatc tgatattgcg aacgaagcca cggctaggta
     2821 tatcctggcg agaacagaag gcactattgg tgaaatcgcg aaactactga cggcggctgc
     2881 gatagtcgct attgaaactg gcgaggaatc agttaatcaa aggacgctcg gacaagccgc
     2941 ttatctgtca ccaaccgagc ggcgacaaac atttgagcgg gcgctattgt gatccccact
     3001 cgctggccgg tacacccagg accaaaagag gccgaaagcc tatcttcttg gttgcgaaga
     3061 ctggccatgg tttacggcct tagcttgaag gatttaattg aacacgacct tggtttctgt
     3121 cggcgacagc agcaacggca ttggctcgat atggcacctc cggctaagct tctgacggcg
     3181 gtggctgcac gtacaggtat ccctttagaa agaatccagc aatcgacaat agctgcactg
     3241 aggcccgtcc tctatgccta ctttgataga aagttttcag cttgcagcac agtgctcatg
     3301 gcacagccaa aggctccgct gcatctcaac ttgcaaaaat ggattctgcg ccacgaactg
     3361 gtgaatgaat tcacggcttg tcgccggtgt ctcgacaact acccaaatgc cgccgtattg
     3421 ctgccttggc ggcttcggat tataacctca tgcccagagc acggcgtaat gctggaagca
     3481 gtgcgaataa ctggagatac tgtggaatgg cgcaaagatg cggaggaatt ggcgcctgcc
     3541 cttgtaagtc ggcttgatct cagaacctgg caagcattga ccaccggtag cgtaaatttg
     3601 actggaggct cagttccggc tggcctctgg tttcgactct tacgaaccat ttgggacgag
     3661 ctgaatagac cccttgaaga atgccacgat aaggcagttt tggctgtttg gaccgggatt
     3721 actagacttc ccttagtgac caaagacttc tggcacttgc gttcggacag gaagcacgcc
     3781 atctgcgtcg gcactgcaat cgatatgcta gagactggtg cagcggtgcc ggaaggaata
     3841 gatggaaggt attttgctgg cagccggagg gg
//
LOCUS       DAOQNZ010000185         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10975_length_3858_cov_4.533000, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000185 DAOQNZ010000000
VERSION     DAOQNZ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10975_length_3858_cov_4.533000"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKW73_04560"
     CDS             <1..512
                     /locus_tag="PKW73_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72583.1"
                     /translation="VENIRWAVGEAQLGLYQFWVENYGYHERDHGPIPFRAELDIHGQ
                     IQRVEGVIRAGATGSASKQELFSFVYNGADVTEVDNHAAYQDEVILEKWSRYIPAVNI
                     LRVEDAASSVEVMIGFMALQSGAMTLDQFVADMKSRRVPEARQKDATLALTQFAKQGI
                     FQEVSPELF"
     gene            595..1926
                     /locus_tag="PKW73_04565"
     CDS             595..1926
                     /locus_tag="PKW73_04565"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNA72584.1"
                     /translation="MSNISEQVLSSAEPQAHIVLGLGYGDEGKGSFVDHLVRKSGARF
                     IVRFNGGAQAAHHVVTEDGREHCFRQFGSGMFVAGTSTILSRYVLIDPQYLLEEAEEL
                     AGKGVCYPLERMIVSADAPIITPFNRLLNQMMEVARSGNRHGSCGFGVGLTQRDVEEL
                     GSKALYVRDLATVELGSKLFQLWQKSLREAEQVACQVNSALYETLKNVDIDYYEQLFR
                     HFYHRVRVLEDEALLALIRENGAIFEGAQGLMLDQNYGTFPHCTRSNCTFENALDLLK
                     DSGFAGEIRRIGLLRGYATRHGAGPLVTVCPDLDLSSCHNGSNPWQGQFRLGWFDAVA
                     ARYALAVVGGVDLLVLTNLDRMATLKEVKIATAYKCADPRFFSESGEMLLPDKGRLDY
                     LAARTEALSGIVPSYMQMPFQLESYLKALEQLLKHPIGAISLSPMHDKSYR"
     gene            2091..2360
                     /locus_tag="PKW73_04570"
     CDS             2091..2360
                     /locus_tag="PKW73_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72585.1"
                     /translation="MTLISILIVVLAIVTGTSAITILGTIYLANRLGNYERALPICFS
                     IGLAAVVAILSFQPSFAAVLLAAYWILGVSAALILGWLVLILSCR"
     gene            complement(2616..2915)
                     /locus_tag="PKW73_04575"
     CDS             complement(2616..2915)
                     /locus_tag="PKW73_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72586.1"
                     /translation="MQAPSGKTPTIFLMTAIHSQADARVFGWQESLEKARQAAQENSL
                     DMSEENYYTHLVIEEMSPGIHCQVQNEWWYSWLDGHWQACPKPDWAQHTTNWSIG"
     gene            complement(2962..3768)
                     /locus_tag="PKW73_04580"
     CDS             complement(2962..3768)
                     /locus_tag="PKW73_04580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNA72587.1"
                     /translation="MNRDKFKTMLLGSAQLKNSKIGARISRITLKLEKFNHLPQWFAF
                     AWAAVYLAIILAIVKGIFPSSIPFTYFELWHTTGNLSDWCTTSWPILAWAIGLTFLVS
                     ILTRNSDEENIYAEYLLRNGFWVSLRAGLMEEILFRWLLLMLWIPMVKVINFIFLGFI
                     DLGLIEIIHLHIIAPIANFFTMDILKEQVGNPNNWSLGAAIIIANSKFRDGHKYLGLI
                     GFVNSWFIGMFMFYLLFKYGLVAAIVVHFLYDLFIYLVRYLDSIFERSRT"
BASE COUNT          848 a          898 c         1098 g         1014 t
ORIGIN      
        1 cggtggaaaa tattcgctgg gctgtagggg aggcccaact ggggctttat cagttctggg
       61 tggaaaacta tggctatcac gaacgtgatc acgggccgat tcccttccgt gccgagctgg
      121 atatccacgg tcaaattcag agggtggagg gtgttattcg cgccggtgcc accggtagcg
      181 caagcaaaca agagctgttc agctttgtct ataacggtgc cgatgtgacg gaagtggaca
      241 accatgccgc ctatcaggac gaagtaattc ttgagaagtg gagccgctac atcccagctg
      301 tcaacatttt gcgagtggag gacgcggcct cttccgtgga ggtcatgatt ggtttcatgg
      361 ctctgcagag tggcgccatg accctcgatc agttcgtggc cgacatgaag agccgtcgag
      421 tgccggaggc caggcagaag gacgctaccc tggcgctcac gcagtttgcc aagcagggta
      481 ttttccagga ggtttctccc gaactgttct aagtttagat agctaagtat ggagcggtcg
      541 ttgtttttcg gcgcggccgc tccgttttcc atgtcgtcaa accgggagga tttgatgtct
      601 aatatttccg aacaagtatt atcctctgcc gagccgcagg cgcacattgt gcttggtctg
      661 ggttacggtg atgaagggaa gggttctttt gtggaccatc tggtgcgcaa atctggtgcc
      721 cggttcattg tgcgcttcaa tggcggtgcc caggccgctc accatgtggt gactgaagac
      781 gggcgcgaac attgtttccg gcaattcggc tccggcatgt ttgtagctgg tacatctaca
      841 attttgtccc ggtatgtgct gattgatccg cagtatctgc tggaagaagc cgaggaactt
      901 gccggcaaag gtgtttgcta tcctctggaa aggatgattg ttagtgccga cgcgcccata
      961 attacgcctt tcaatcgcct cctcaatcag atgatggagg tggcccgtag cggcaaccgc
     1021 catggttcct gcggctttgg tgtgggtctg acccagagag acgtggaaga gttgggttct
     1081 aaggctcttt atgtcagaga tctggccacg gtagaactgg gaagtaaatt gtttcagctc
     1141 tggcaaaaga gcctgcggga agcggagcaa gtagcctgcc aggtgaattc ggcactgtat
     1201 gaaaccctga agaatgtgga tatcgattac tacgagcagt tgtttcgtca tttctatcac
     1261 cgcgtcaggg ttctggaaga cgaggctcta cttgcattga taagggagaa cggcgctata
     1321 ttcgaagggg cccagggtct tatgctcgat caaaactacg gcacgtttcc acattgcaca
     1381 aggtctaact gtacatttga aaacgcgctg gacctgctga aggacagtgg cttcgctggt
     1441 gaaatccggc ggattggtct tttgcggggc tatgccacca ggcacggtgc cggtcctctg
     1501 gtaacggttt gcccggacct tgacctttcc tcctgccata acggcagtaa tccctggcag
     1561 ggccaattcc gcctcggttg gtttgatgcc gttgccgctc gctatgctct agctgtagtc
     1621 ggtggtgtgg atctgctagt gctcactaac ctggaccgca tggctacact caaggaggtc
     1681 aagattgcta ccgcctacaa atgtgccgac ccgcgatttt tcagcgaaag tggtgaaatg
     1741 cttttgcctg ataaggggcg gttggactat cttgctgccc gtacggaagc cctgagtggg
     1801 attgtgccgt cttatatgca gatgcccttt cagttggagt cctatctgaa agccttggag
     1861 cagttgctga aacatcctat tggcgccatt tctttaagtc cgatgcacga caagagttat
     1921 cgttagaagc ggtgcctgtt ctgccacgag gcaagcactt caggtgtttt cttgcgtgcc
     1981 ggcgccgcta ttttattgcc aatttcggct gagcgtaatt ttgaaagctt acgcagccgg
     2041 ttcattaacc aaacagggaa gaccattccc cactagaaca ggagttctcc atgacgctca
     2101 taagcatctt aattgttgtt cttgccatag ttaccggtac ctcggccatc accattttgg
     2161 gtaccatcta cctggctaat cgccttggta attacgagag agcactgcca atctgctttt
     2221 ccataggcct tgccgccgtg gttgcaatcc tctccttcca gccttccttt gccgccgtgc
     2281 ttcttgccgc ttattggata ctcggtgtat cggcagcttt gattctgggc tggctggttc
     2341 tgattctcag ctgtcgctga aggctgactt agccgggttt gggagtcgtt taggtaaggc
     2401 tgcttagata gcgcctgact aaaatacggc tcccaagcca ctttttttgt tcttttgcat
     2461 tttgagtact aaagaagaca gtaggctcgg cgaaaagcga aaatgaggga gcttgcggga
     2521 gcctgaggca aatggcgata aattgtgcca accaggtgct gattaagctg aacctggttg
     2581 gccggggtta aggttaaagc gctcctagag ggccatcaac cgatggacca gttggttgtg
     2641 tgttgagccc aatcgggttt ggggcaagct tgccagtgtc cgtccagcca ggagtaccac
     2701 cattcgtttt gcacctggca gtgaatgcct ggggacattt cttcaatgac caggtgggta
     2761 tagtagttct cttcgctcat atccaggctg ttttcttgtg ctgcttgcct ggccttttcc
     2821 agagattcct gccagccgaa aactcttgcg tcggcttgac tgtggatggc tgtcataagg
     2881 aaaattgtcg gggtttttcc gctaggtgct tgcatagatt ccttgtcttt gattcagcgg
     2941 tgaatgagca cgctgatgcg gttaggttct ggatctttcg aagatgctgt ccagataacg
     3001 caccaggtag ataaagaggt cgtagagaaa gtgcacgacg atggccgcta ccagcccgta
     3061 tttgaagagc aggtagaaca tgaacatacc gatgaaccag ctatttacaa agccgatcaa
     3121 gcccaggtat ttatgcccgt ctctgaattt ggaattggca atgataatgg ccgcacccag
     3181 ggaccagttg ttggggttgc cgacctgttc cttgaggatg tccatggtga agaagttggc
     3241 tatgggcgcg atgatatgca aatggattat ttctattagc cccaagtcaa tgaaacccag
     3301 gaagataaag ttgatgactt tgaccatggg aatccagagc atgagaagca gccagcgaaa
     3361 gaggatttct tccatcagac cggcacgcaa gctcacccag aagccattgc gcaagagata
     3421 ctcggcatag atgttttcct cgtcactgtt gcgggtgaga atgctgacaa ggaaggtgag
     3481 acctatggcc caggctagaa tcggccagga tgtggtgcac caatcgctga ggttgcctgt
     3541 ggtgtgccag agttcaaaat aggtgaaggg tatgcttgag gggaagatgc ccttcacaat
     3601 agccaggatg atggccaggt aaactgccgc ccaggcgaag gcaaaccact ggggtagatg
     3661 gttgaacttt tccagcttta aggtaatgcg agagattctt gctccgattt tgctgttttt
     3721 gagttgagct gaaccaagca gcatggtttt aaatttgtct ctattcatgg cgtttcgttt
     3781 ggatagggtt aaaaagacag gggcttaaac gattgcttga gcccctgatg tatggtgtcc
     3841 gttgagaagc ggctactt
//
LOCUS       DAOQNZ010000186         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_10988_length_3856_cov_3.804262, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000186 DAOQNZ010000000
VERSION     DAOQNZ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10988_length_3856_cov_3.804262"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..822
                     /locus_tag="PKW73_04585"
     CDS             157..822
                     /locus_tag="PKW73_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72588.1"
                     /translation="MASTFSQIKRYFSSLPPGTLISTRELLPFGKRGSVDWATHTLVN
                     RQIIERLAQGVFRLRLPGNKPVSVAKIAEFKARIFGRVLVSFGTVLVEDIKHMMPGLE
                     KDTVVFAISGRTSAFRTSKNRVVLLGMSKRKIALGNSEIGKAMRDLWFVGKRNRPSRA
                     TFEDIEPLVEKHKRALPWYKPLMPQWQTDRYLYKQFLHPLGTIPKQFHLDLWSVKEPL
                     STL"
     gene            954..2072
                     /locus_tag="PKW73_04590"
     CDS             954..2072
                     /locus_tag="PKW73_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA72589.1"
                     /translation="MSLNHYVTLGHSGLRVSPLCLGAMTFGSEWGFGSSEKESHEILD
                     LFFEHGGNFIDTANVYTKGHSELILGNYLNKPGFRRDRAVVATKFFGTLHPGDPNAGG
                     ANRKAIFAALEASLRRLNTDYIDLYWMHCWDKFTPIEETMQALDDLVKQGKVRYIGFS
                     DTPAWKTAQAQVTAKMRGWAPLIALQIEYSLLERTVEGELLPMAKEMGLGVTPWSPLK
                     FGILTGKYSRENKGKVDPARGDWVLHHLGKEKTYEVIDALINVAQEVGSTPARVALAW
                     VQARSGVTSSIIGARTKEQLLDNLGCLEINLSQAQIQQLDAVSAPTLNFPHDFLKGAG
                     VFMAAETTINGETNGESKLAPKSDAERFDRLEKQASKV"
     gene            2411..3622
                     /locus_tag="PKW73_04595"
     CDS             2411..3622
                     /locus_tag="PKW73_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA72590.1"
                     /translation="MTTSFNRNRPDNLLVHNRENRLHNLDTFAPLSGPYVMHNGRSLI
                     SFASNDWLGLSSHPCLKENAIRFVTRYGVNNASSHLSEGNIDGYDRVEKKLADLTGFE
                     TALLFQSEAKLLEVLFSELFSSRSVIAVDRLMPRSTAQTLAKHSAETRAKWFSYRHWQ
                     HEDARVRLCSEIYDDCQEKWLLNQSLYALDGSVESVPALEKMHRNFGIKVLLDDSNAA
                     GVLGVNGMGLASYIAGNGLAFGSFARGLGCAGAYLLCSHEVKSQLLDICKASIYAVPM
                     QPSALGAIDAALDIVPGMHKERLQLLQMAEDFRKVVLKMGFETGPSSTQIISILAGTD
                     SQAIRIAEHLYDNGIFAAPIRPSNLAQGNARLRISISLKHEKEHIFRLLRCLSNFRKP
                     PISEKTSGTCS"
     gene            complement(3629..>3856)
                     /locus_tag="PKW73_04600"
     CDS             complement(3629..>3856)
                     /locus_tag="PKW73_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72591.1"
                     /translation="GSASKNYAAGRPVEDDEQFSKKKGFVMGVGRGLPGGAIIQGVAT
                     SGKDLELHKGDILTVSLREDLEVLEKRPHGR"
BASE COUNT         1043 a         1015 c          925 g          873 t
ORIGIN      
        1 ccaggtatcc ttcggcaaca actccggaag cactaagaaa tttggagaaa aatcttagtg
       61 cttccttcaa agaaaaaaga ctatatcgga tttgaacttt ttctcttctg ccaccgtttt
      121 ctaaataagc ggaggaaaac aaaaggaggg aaaagtatgg ccagcacttt ctcgcagata
      181 aaaaggtatt tcagctcact gccgccaggc actctgatta gcaccagaga gctactgccc
      241 tttggcaaaa gaggtagcgt agactgggcg acccacaccc ttgtcaacag acagattatc
      301 gagagactgg cacaaggtgt ttttcgcctg cgcttgccgg gcaataaacc tgtcagcgta
      361 gccaagattg cggaattcaa agcccggata tttgggcgcg tactggtttc cttcggcacg
      421 gtcctggtcg aggacataaa gcacatgatg cctggtctgg agaaggacac tgtagttttc
      481 gccatatcag gacgaaccag tgccttccga accagcaaga atcgcgtagt acttttgggt
      541 atgtccaaac gaaagatagc attgggaaat tcagaaatag gaaaggcaat gcgcgatctg
      601 tggttcgtag gcaaacggaa ccgcccgtct agagccactt tcgaagacat cgagccgctg
      661 gtggaaaagc acaaaagagc actaccctgg tacaaaccgc tgatgcctca atggcaaaca
      721 gatcgctacc tgtataagca attcttgcat ccgctcggta ccattccaaa acaatttcac
      781 ttagacctct ggtcggtgaa agagcctctc agcacgctct gaaatcctcc cggcagacgc
      841 aggaaaagta gagcaagcat aaagctaatc tgacaattca atgcctctgg caagaacttg
      901 gttagaattg ccctcgggca atcagaactc tagtcgaggt acggagacgg aagatgtcat
      961 tgaatcacta tgtcaccctg ggtcattccg gtctgagagt cagtcctctc tgcctgggtg
     1021 caatgacatt cggttctgaa tggggcttcg gttccagtga aaaggaaagc cacgaaattc
     1081 tcgatctctt ttttgaacat ggcggcaact tcatagacac cgccaatgtc tacacaaagg
     1141 ggcattcaga actaattcta ggaaattact tgaacaagcc cggctttcgc agagatcgtg
     1201 cggtagttgc caccaagttc ttcggcactc ttcacccagg tgatcctaat gccggcggag
     1261 ccaatcgcaa ggcaattttt gccgctctgg aagcctcact gcgcagactt aataccgact
     1321 atatagatct atactggatg cattgctggg acaaattcac ccccatagaa gaaaccatgc
     1381 aggccctcga cgatctggtc aagcaaggga aagtacgata catcggattc tccgatactc
     1441 ccgcctggaa gacggcgcag gctcaggtga cggctaaaat gagaggctgg gcaccactta
     1501 tcgctttgca aattgaatat tcccttcttg aaagaacagt agagggggaa cttctaccaa
     1561 tggcgaagga aatgggcctt ggagtaacgc cctggtcccc gctgaaattc ggaattctga
     1621 ctggcaagta cagccgcgag aataaaggca aagtagatcc ggcaaggggc gactgggttt
     1681 tgcaccatct gggcaaagaa aagacctacg aggtaataga cgcccttata aatgttgccc
     1741 aggaagtagg ctcaacaccg gctcgggtag ccctggcttg ggtgcaagcg cgcagtggag
     1801 taacatcttc aatcatcgga gcccgaacaa aagagcagtt gctagacaat ctgggctgcc
     1861 ttgaaatcaa tttgagccag gcacaaatac agcagttaga tgcagtttct gctcctactc
     1921 tcaattttcc ccacgacttc ttgaaaggtg ccggtgtgtt tatggcagct gaaaccacca
     1981 tcaatgggga aacaaatggg gagagcaagc tcgcaccaaa atccgacgcg gagagattcg
     2041 atcggcttga gaagcaggca tcaaaagttt aagaaaacaa gacaaccact ctgttcaaac
     2101 tttgcctaag tgcatcagag aagcaagagg taagcgtccg aaattcagcg aaagaggcac
     2161 tgccactgaa tgcagtgcct ccttgttgag cgcctctgca agcggaatcc ggctctgtaa
     2221 cttttgttgt tcatgtaaca atttctcgtt cacatcgact gataaaaaca gacagagacc
     2281 agaaaccaag gcgagcagcg attttaccgg caccactagc ggtattaatc caggaaatct
     2341 ttttagtcgg caagtagtct cggaccggtc gaatgaatat acaatcgact cactcgagaa
     2401 aggagcagct ttgactacca gtttcaatag aaaccgtcct gacaatcttt tagtacacaa
     2461 tcgagaaaac agacttcaca acctcgatac cttcgccccc ctgagcggcc cctatgtcat
     2521 gcacaacggc cgttctctca tttctttcgc cagcaacgac tggttgggtc tttcctctca
     2581 tccctgcctg aaagaaaacg ccatcagatt cgtcacccga tatggtgtaa acaacgcgtc
     2641 ttcacatttg agcgaaggca acattgacgg ctacgacaga gtggaaaaga aactcgccga
     2701 cctgactggc tttgagacag ccttgctctt tcagagcgag gcaaagcttc tggaagtact
     2761 attctccgaa cttttctcca gccgctctgt aatcgccgta gatagactga tgcctagatc
     2821 cacggcccag acattagcaa agcacagcgc cgaaaccaga gcaaagtggt tcagttaccg
     2881 acactggcaa cacgaagatg ctcgggtgag gctgtgttca gagatctatg atgattgcca
     2941 ggaaaaatgg ctcctcaatc aatctcttta cgcccttgat ggttcagttg aaagtgttcc
     3001 ggctctggaa aagatgcacc gtaacttcgg catcaaggtt ctccttgacg attccaatgc
     3061 cgccggcgta ttgggcgtga acggcatggg tctggcctcc tacattgccg gtaatggtct
     3121 ggcgttcggc agctttgctc gcggtctggg ctgcgccggc gcttacctcc tctgctccca
     3181 tgaagttaaa agccaacttc tagatatctg taaagcaagt atctacgccg tgcctatgca
     3241 gccatccgcc ctcggagcca ttgacgccgc cttagatatt gtgccgggca tgcacaaaga
     3301 aagactgcaa ctgctgcaaa tggccgaaga cttccgcaaa gtagtcttga aaatggggtt
     3361 tgaaacaggt ccctcgtcca cacaaattat ttccattctg gcaggcaccg actcgcaagc
     3421 cattcgcatt gccgagcatc tctacgacaa cggcattttt gccgccccca ttagaccaag
     3481 caacctggcg caaggcaacg ccaggctgag aatctctatt tccctgaagc atgagaagga
     3541 gcatattttc agactcctgc gttgcctctc caatttccgc aagcccccga tctcagagaa
     3601 gacctcaggt acctgtagct aatggctctc aacggccatg cggacgtttc tccagtactt
     3661 ccagatcttc ccgcaaactg acggtgagga tatcgccctt gtgcaattcc agatctttac
     3721 cgctggtagc cacgccttga atgatggcac cgccgggtaa acccctaccc actcccatga
     3781 caaagccctt cttcttcgag aactgctcgt catcctccac aggtcttccg gcggcgtagt
     3841 tcttgctggc gctacc
//
LOCUS       DAOQNZ010000187         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11028_length_3849_cov_3.294939, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000187 DAOQNZ010000000
VERSION     DAOQNZ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11028_length_3849_cov_3.294939"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKW73_04605"
     CDS             complement(<1..603)
                     /locus_tag="PKW73_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA72592.1"
                     /translation="MEVSHRLKSIPPYVFAEIDKKRQAAVAAGVDVINLGIGDPDQPT
                     PRHIVEAMHDALENPANHHYPPFGGTKEYKQAISQWMKKRFGVEVNPDKEITSLIGSK
                     EGLHHTIMAFVDKGDINIIPDPAYPVYRTSTILAGGEPYYMPLTPENNFIPDLEAIPE
                     AVLKKAKLLMLNYPGNPTAGIADLAFFEKAVAFCKKHNILL"
     gene            914..2623
                     /locus_tag="PKW73_04610"
     CDS             914..2623
                     /locus_tag="PKW73_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA72593.1"
                     /translation="MSSTDNSSASPPDSPLRLPVLLAVLVVGAAVIFAQLGKFPLFNP
                     DEALYAEPAREMLEIKEYITTYLNYVVRFTKPPLVIWAMALSMNLFGVNEFAARFFGA
                     ATGVALIGVTYLFASRYISTTAAFLAGFSLIMAPLYLGTAREAITDMPLAFFMASAQL
                     AFFHAFFQKRKVYALLGFAMVGLSIMTKGPVGMVLPLGILFAFHLLRGDLKEAWKAYS
                     PVLGLILTAVIAVPWFAVEIYVTKGAYFQEFIMRENFQRFTAVVDAHKQPVWYHLAAM
                     VVGFMPFSVFLPGLVKDYLTRLTLTLKTPSESIWERYRHLASSLRNLGTAESLMVYSA
                     LWAFITLAFYSASVSKLLPYTLPAFPALALLVSHYIDRQIKRERFLPLALPLVALVAL
                     YGGVGLVAPHLTGRLRDCPAELPPLVIGFAGFGAGVTFLSILALRLKKMRLGMCFFAV
                     GTTLGLAYYAVRILPVVSAKWEGGLPELSAFAGQSSLPIIVYDMRKPGVPFYARRQVE
                     NINNHDVFLVRLSQLEKAYVLTRTKRLEALSSIAGLKPVGSQGSFTLLLWGQVKESQP
                     VNK"
     gene            2632..3435
                     /locus_tag="PKW73_04615"
     CDS             2632..3435
                     /locus_tag="PKW73_04615"
                     /inference="COORDINATES: protein motif:HMM:NF025428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HNA72594.1"
                     /translation="MLGSGIPPNTRQLLLEQLKRFQVDVSQLKLVDKDLPMGFYAVPV
                     LAEQATGAWHALRALHKESGYWPILVQTESLLDELLSTLERHDDDFDEYMRDTIAEGL
                     TIDFPNWLRNQEPDPRDNDELMGEWPEDAEPAHVLVQLLDLKVDDEGRFSMEQLSEVL
                     ILLCPLKEPWHLPALLKLGGWNACPDAAHHLAAFKYWFDKHGAEPAFFSMDTFEFIVT
                     NPPTTEEEATSLAIEQYFYCFDLVAQGCGSILELAGNLVDSTVWYFWWD"
     gene            3446..>3849
                     /locus_tag="PKW73_04620"
     CDS             3446..>3849
                     /locus_tag="PKW73_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72595.1"
                     /translation="MEPQLEQAFKNSGSWFFWIAGCTLVNVSMTLTNGNFGLALGVSL
                     VDVFPALLKAFSAPNEVFLISSLCLAAVFIGLFGLLGKFASEGKSWAFIVGMLVFGVD
                     TAICGLLQDWMGLGVHLFALYSLFIGLKLALST"
BASE COUNT          738 a          960 c         1053 g         1098 t
ORIGIN      
        1 caggaggatg ttgtgctttt tgcagaaagc tactgctttc tcgaagaaag ccagatcagc
       61 tatgccggct gtggggttgc cggggtagtt gagcatcaag agcttggcct tcttaagcac
      121 cgcttccggt atcgcttcca gatcgggaat gaagttgttt tccggcgtca ggggcatgta
      181 atagggttcg ccgccggcca gaatggtgga agtgcggtaa accgggtatg ccggatcggg
      241 aattatgttg atatcgcctt tgtcgacaaa ggccataatg gtgtggtgca gaccttcttt
      301 tgagccaatc aaagaggtga tttctttgtc gggattgact tccacgccga agcgtttctt
      361 catccactgg gagatggcct gtttatattc tttggtgccg ccgaaaggtg ggtagtggtg
      421 attggcggga ttttccaggg cgtcgtgcat agcttccact atgtggcgcg gcgtgggttg
      481 atccgggtcg ccaatgccca ggttgattac gtctacacct gctgctactg ctgcttgccg
      541 tttcttatcg atttcggcga agacgtaggg agggatggac ttaagtctat gagaaacttc
      601 cataattacc gacctttagt tcttagaaca tctgacatta tcatagcttc acatggaaag
      661 ccgccatccg tcccaactag gaattagagt taacgttctt cttaatatgc cttagaatct
      721 gcatcagaac gggcgccggg cttcctcttt gcgaccccgc cgcgagcctt cgccggtgca
      781 gtacagcccg agggctcccg ggggcggcca tgaaatttat ttattggtct tgctagagtt
      841 ttgaaacttc cccggtcatg gtctaacctg tttggatcag ttgctgccaa gccgacagaa
      901 acaggaatcg aaaatgtcat ccacggataa tagctccgca tccccgccgg actctccctt
      961 gcggctgcct gtgctgttgg cggtccttgt agttggcgcc gccgtcattt ttgcccaatt
     1021 gggcaagttc ccgcttttca atcccgatga ggctctctat gccgaaccgg caagggaaat
     1081 gctggaaatc aaggagtaca taaccactta tttgaattat gtggttcggt tcaccaagcc
     1141 gcccctggtc atctgggcca tggctctcag catgaacttg ttcggggtta acgagtttgc
     1201 cgctcgcttc ttcggtgcgg cgaccggggt ggctttgatt ggcgttactt acctctttgc
     1261 cagtcgctat atctccacca cggctgcttt cctggccggt ttcagtttga taatggcgcc
     1321 cctctatctg ggcacggcgc gggaagcaat tactgatatg cccctggctt tctttatggc
     1381 ctcggcacaa ctcgccttct ttcatgcctt ttttcagaag cgcaaagtct atgccctgtt
     1441 gggtttcgcc atggtaggtt tgtccatcat gaccaaggga ccggtgggaa tggtgctgcc
     1501 actgggcatt ttgttcgcct ttcacctgct gcgcggtgat ttgaaagagg cctggaaggc
     1561 ttacagcccg gttcttggtc tcattttgac ggccgttatt gccgtaccct ggtttgccgt
     1621 ggaaatttat gtgaccaagg gggcttattt tcaggaattc atcatgcgtg agaattttca
     1681 gcgcttcact gccgtggtgg atgcccataa gcagccggtc tggtaccacc ttgcggccat
     1741 ggtggtgggt tttatgccct tttctgtctt cctgccggga cttgtcaaag actatttgac
     1801 cagactgact ctaaccctga aaacccccag tgagagcatt tgggagcgtt atcgccacct
     1861 tgcctccagt ttgcgaaacc tggggacggc tgagagcttg atggtttatt cggcgctctg
     1921 ggccttcatc acccttgcct tttattccgc ctcggtgtcc aagcttttgc cttacactct
     1981 gccggccttc ccggcattgg cccttttggt ttctcattac atcgacagac agatcaaaag
     2041 agaaaggttt ctacccctgg ctctgccgtt agtggcactg gtcgctctct acggcggtgt
     2101 cggtctggtg gcgccccatt tgaccggtcg cctgcgcgat tgcccggctg agcttccgcc
     2161 tcttgttatt ggttttgccg gtttcggagc cggtgttact tttctttcaa ttctggcttt
     2221 gcgtctcaag aaaatgcgtc tgggcatgtg tttctttgcc gtgggcacta cacttggtct
     2281 ggcctattat gccgttagaa ttttgcctgt ggtttcagct aagtgggaag gcgggctgcc
     2341 cgagctttcc gcttttgccg gccagtcttc tctgcccatt attgtttacg acatgcgtaa
     2401 gccgggagtg cccttctacg cccgcaggca ggttgagaat atcaacaatc atgatgtttt
     2461 cctggtgcgg cttagccagc ttgaaaaggc ctatgttctc accaggacaa aacggctgga
     2521 agcactgtcc tctatagccg ggttaaagcc ggtaggaagt caggggagtt tcactctctt
     2581 gctttggggg caagtgaagg aaagtcagcc cgtaaataag tagacccgtt tatgctcgga
     2641 agcggcatcc cgccgaatac acgccaactt ttgctcgaac agttgaagcg ctttcaagtc
     2701 gatgtttctc agctaaagct ggtggacaag gatctgccta tgggatttta tgcggtgccg
     2761 gtactggctg agcaggccac cggtgcctgg cacgcattga gggctttgca taaagagagc
     2821 ggctactggc ccattctggt tcaaaccgaa agccttctgg atgagttgct ttccactcta
     2881 gagcgacatg atgacgattt tgacgaatac atgcgggaca ctattgccga aggactgacc
     2941 atagattttc ccaattggtt gagaaatcag gagccggatc ctagagataa cgatgaactg
     3001 atgggcgaat ggcccgagga tgccgagccc gctcatgtgc tggtgcaact gctggatttg
     3061 aaagtggacg acgaggggcg tttttccatg gagcagttga gcgaagttct catcttgctt
     3121 tgtcccttga aagagccctg gcacttgccg gccttactca aattgggtgg ttggaatgcc
     3181 tgtcccgatg ccgcgcacca cctggcggcc ttcaaatact ggtttgacaa acacggcgcg
     3241 gagccggcat ttttctccat ggatactttc gagttcatcg tcaccaaccc tcccaccact
     3301 gaggaggagg ctacttcgct ggcaatcgaa cagtatttct actgctttga tctggtagcc
     3361 cagggttgtg gttcaattct tgagctggcc ggtaacctgg ttgactctac ggtctggtat
     3421 ttctggtggg attaaggaga gtaagatgga gccgcaactt gagcaagcct ttaaaaattc
     3481 tggtagttgg ttcttctgga tcgccggctg caccctggtc aatgtctcca tgactctgac
     3541 caacggcaat ttcggtcttg ccctgggggt atcgctggtg gatgtctttc ctgctttgct
     3601 gaaggccttc agtgctccca atgaagtgtt tctgatttca tctctttgtt tggcagccgt
     3661 cttcatcggc ttattcggac ttcttggaaa atttgcctcc gaaggtaaga gttgggcttt
     3721 tatcgtcgga atgttagtgt ttggtgttga taccgcaatt tgtggtctgc ttcaggattg
     3781 gatgggactg ggggtgcatc ttttcgcact ctatagtctt ttcataggct tgaaattggc
     3841 tctctcaac
//
LOCUS       DAOQNZ010000188         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11104_length_3836_cov_4.065856, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000188 DAOQNZ010000000
VERSION     DAOQNZ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11104_length_3836_cov_4.065856"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1185
                     /locus_tag="PKW73_04625"
     CDS             151..1185
                     /locus_tag="PKW73_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA72596.1"
                     /translation="MEFRQLGNCGLKVPVLSFGAATFGGGTEFFKEWGATDVKEATRM
                     VDLCLDAGCNFFDTADIYSRGLAEEILGEAVAGRRQNVLISTKATFAMSDEPNNVGSS
                     RYHLIKACEDSLRRLKTDYIDVYFMHGFDALTPVEEVLSSLDQLVRSGKVRYIGCSNF
                     SGWHLMKSLSISKQYGWSGYVAHQAYYSLIGREYEWELMPLAKDQNLGTMVWSPLGWG
                     RLTGKLGRGKDLPQQSRLHKTAEKGPQVDNDYLYRVTDTLEEIAEETGKTVAQIALNW
                     LLTRPTVTNVVMGARNEEQLRQNLGAVGWSLSTEQIAKLDEASALTPIYPYWHQWQFQ
                     ERNPLPFKTP"
     gene            1186..2565
                     /locus_tag="PKW73_04630"
     CDS             1186..2565
                     /locus_tag="PKW73_04630"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HNA72597.1"
                     /translation="MKLKAAARQWSNYLLAEINPAGLVSFRIVFGLIMSLDMLLYLLM
                     NRVHSKYIDPQFLFLYIHSMARPAPALLEALFAALAVAALFMAIGLFYRFSAWFFCLG
                     LTYIFLLDRAQYLNHMYLMCLLSLLLAVSQAGHCFSLDNYLRKNPNLPVPRWNHFILR
                     FQLFVVYLYGAIWKLNPDWLRGEPQRFWFTNRAAEPFIGGIVSQEWFVWLVTYGGILT
                     DFTLPFLLIFPRTFWYGVAIATFFHTTNSLLFDIGVFPWLMLGTIVLFPPPDWPYKAA
                     SRLSVWLKGPVILPEVSVQEGGQALKTNWKQLAALVFVHVYMIFHVVFPLRHFLYPGN
                     VDWNEEGYYFAWRMMLHHKEAQLVIYKEDPASGDISVVDTHDLLTRRQRMIMKQRPEL
                     IHQFCVWLADREEKRTGKRPRITVRSTASLNKHQWQELIDPKVDLGAEKFSLDHKSWI
                     VDMKEEGKP"
     gene            3389..3736
                     /locus_tag="PKW73_04635"
     CDS             3389..3736
                     /locus_tag="PKW73_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72598.1"
                     /translation="MTFNPNKYSSIQTLAIALTAAICVTALSLMTASYWLLPVAAWIG
                     FASYSNVYTGSMKDSLYWTAAVLTMLSVMLISGAGAFSTVLILIGGGIASAIAGAAFA
                     LGHKTGSDLAFTS"
BASE COUNT          938 a         1073 c          929 g          896 t
ORIGIN      
        1 cgaattgaaa gtcagcaccc cgaattggaa ttctgatccc cggattggac cggaggcggg
       61 tggggaggag ccgcgacctc cacgagaaaa ggaaacgctt ccatacgcga gaaccggata
      121 aaataagcaa aactctcatg gaggcccata atggaattca gacagcttgg caattgcgga
      181 ctgaaagtgc cggttctaag ttttggcgct gccacttttg gcggtggcac cgagttcttc
      241 aaggaatggg gcgccacaga cgtgaaggag gccaccagga tggtggatct ctgcctggac
      301 gccggctgca atttcttcga tactgccgat atttattccc gtggtctggc ggaagagatt
      361 ctgggagaag ccgtggccgg acgccgccag aacgttctca tttccaccaa ggctaccttc
      421 gccatgtccg acgaacccaa taatgtcggc tcttcccgct accacctgat caaagcctgt
      481 gaagacagtc tgcgccgcct caaaacagac tatatcgatg tctacttcat gcacggcttc
      541 gacgccctca cccctgtaga agaagtcctc tccagcctcg atcaactggt gagaagcggc
      601 aaagtacgct acataggctg ctccaacttt tccggctggc atttgatgaa atcactttct
      661 atcagtaaac agtacggctg gagcggttat gtggcacacc aggcgtatta cagcctgatt
      721 ggtcgcgaat acgaatggga acttatgccc ctggcaaaag atcagaacct gggcaccatg
      781 gtctggagcc ccctgggttg gggacgcctg accggaaaac tgggcagagg caaggacctg
      841 ccccagcaaa gccgcctgca caaaaccgcc gagaaaggtc cccaggtaga taacgattat
      901 ctctacagag tcactgatac actggaagag atagccgaag aaacaggcaa aacagtagcc
      961 cagattgctc tcaattggct acttacccgg cccaccgtca ccaatgtggt gatgggagct
     1021 cgcaatgaag aacaactccg gcaaaatctc ggcgctgtcg gttggagttt gagcactgaa
     1081 caaatcgcta aattggacga ggccagcgcc ctcacaccga tttatcctta ctggcatcaa
     1141 tggcaattcc aggaacgcaa tcccctgccc ttcaaaaccc cctaaatgaa gctaaaagca
     1201 gccgcccgcc aatggtccaa ctatctgctc gccgagataa acccggccgg tctggtcagc
     1261 tttcgcattg tatttggctt gatcatgagc ctggatatgc ttctctacct gctcatgaac
     1321 cgggtgcact ccaaatatat cgatccgcag tttctctttc tctacattca ctccatggcc
     1381 agaccggctc ccgccctgct ggaagccctc ttcgcggccc tggccgtagc cgcgcttttc
     1441 atggccatcg gtctctttta ccgcttcagc gcctggtttt tctgcctcgg tctgacctac
     1501 atttttctgc tcgatcgcgc ccagtattta aatcacatgt atttgatgtg cctgctctcg
     1561 ctgctgcttg ccgtgagtca agccggccac tgcttcagcc tcgacaatta cctcaggaag
     1621 aacccgaatc tgcccgtacc gcgctggaat cactttatct tgcgcttcca gctttttgtg
     1681 gtctacctct acggagcgat ctggaagctc aatccagact ggttaagagg tgagccccaa
     1741 cgcttctggt tcacaaaccg agcagctgag ccgttcattg gcggcatagt cagtcaggaa
     1801 tggtttgtct ggttggtcac ctacggcggc attctcaccg actttaccct accttttctg
     1861 cttatttttc cgcgcacatt ttggtacggt gtggccatcg ccactttctt ccataccact
     1921 aactcccttc tcttcgatat cggcgtcttc ccctggttga tgctaggcac cattgtgctc
     1981 tttcctccgc ctgactggcc ctacaaagca gcaagccggc tttcagtctg gctgaagggg
     2041 ccggtgatac taccggaggt gtcggttcag gagggcgggc aagcgctcaa aacaaattgg
     2101 aaacaattag cagccctcgt tttcgtgcac gtttacatga tattccatgt agtatttccg
     2161 ctgcgtcact tcctctaccc cggcaatgta gactggaacg aagaagggta ttacttcgcc
     2221 tggcgcatga tgttgcacca taaggaagcc caactggtca tatacaagga agatccggct
     2281 agcggcgata tttccgttgt cgacacccat gacttgctga ccagaagaca gcgcatgatt
     2341 atgaaacaaa gaccggagct aatccatcaa ttttgtgtct ggctggccga ccgagaagag
     2401 aaacggacgg gtaaaagacc acgcattacg gtgcgttcca ctgcttccct caacaagcac
     2461 cagtggcaag aattaatcga tcccaaggta gatctgggag cagaaaaatt cagtctcgat
     2521 cataaaagct ggatcgtgga catgaaagag gaaggtaagc catagttacc gcccagacgg
     2581 caatcacctc tgtgatagaa ggttaagcct gggcgggccg cttgttggca gtagcgcggg
     2641 actatttgcg tggggcgacc atcccccatg gacttgtgga aggaaggcaa gaatcgagag
     2701 tgctcacact gtcgcgtggc ggcaaaaggt cttgccgcgt acataaatcc aggcacatga
     2761 atttacaggc cggtaaaagc tttacggtgg agacagcaag aggatgccaa gcgcagccgg
     2821 caccaaaaat ccagtatgtg caagggttta cgggcgcccg ctcctgtgac gactcgctaa
     2881 ttggtcccaa attacattgt acttagctag gcgcgcactg ataccactgg aagtgccata
     2941 aaacagacac aagtgcacac aagcgcgtca cggcgccgct ttcagagtca gatcaaccta
     3001 tgcctgcgcc agttttatca acttgccata gactatcgct ttctataaag cttgggtatt
     3061 ccccttcttc attcgtttaa aagaaaaccg ataagcaaca ttttcgctgc acaagcaagt
     3121 tcccttagcg caattcatat gcgctgggcg gtcatcgagg ccaacgacaa cactaaaaac
     3181 gacaccgatg tactgcacca ggagcttatc ttcggcttgc ggacgcccta tattatggcg
     3241 accgtcttcc agtagccccg gttcaacttg taagccccaa agaaaatatc attgcctgga
     3301 agcggtgaaa agcaatgcac cggtcaaaag cagcgggaaa gctgctttta ccgttcacgt
     3361 tcacattcat tcaatttgag gaatcaccat gactttcaat ccaaacaaat actcttccat
     3421 ccaaacactg gccattgctc ttacagccgc catctgcgtt acagcacttt cactgatgac
     3481 cgccagttac tggcttttgc cggtagcagc ttggatcggc tttgcttctt acagcaatgt
     3541 ctacaccggc agtatgaaag actcccttta ctggactgcg gcagtactaa ccatgctgtc
     3601 cgtcatgctc atttccggag ctggtgcatt ctccactgtt ttgatactga taggcggcgg
     3661 tattgccagc gccatcgccg gtgccgcctt cgctctgggc cacaaaaccg gctcagacct
     3721 ggcttttacc tcctgacact agaagcggcc gttcagtaca agtcactgtc cagaatagtc
     3781 ctccgaaaaa acttcttccg aatttcacat agtcacttgc aattactact taagga
//
LOCUS       DAOQNZ010000189         3824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11165_length_3824_cov_3.550279, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000189 DAOQNZ010000000
VERSION     DAOQNZ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11165_length_3824_cov_3.550279"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PKW73_04640"
     CDS             <1..891
                     /locus_tag="PKW73_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNA72599.1"
                     /translation="LRNQSGAMASVLLLLTCLGLLIVGSIGIDVSHAFYVRAQLQTVA
                     DNSALTGAYYLCSLMPGQADVKRSEEMARKMAARSIVDGRYFEDDGENSTLSYASEVK
                     CGSAPQVCRISMTRPVPTSFGRLVGCHTIPVSVSSQAGAFCGTRTVAANWLTNLAISH
                     KVQKENKLDLDPSSEEHNAWFIQEWSGQSNPVVKMGSTSVSRGPVNLASLEKGRVYNV
                     AIVKGEGAKGENRLPPSSEVIGTTSIIVIATESATSARIKVLPGGLIKGQPGSYLVPG
                     STGDVQFVLRNGHWRVALMK"
     gene            1268..1732
                     /locus_tag="PKW73_04645"
     CDS             1268..1732
                     /locus_tag="PKW73_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72600.1"
                     /translation="MSSGFGPTSITSASGRLFEIRSELYIDNVDETSFAFFIDEPKFE
                     KERAYYVIGIGRTILRDWQIQDKLTVSKEVGLALLNYYNRKGMDIDKLVWNQYPGPDD
                     DNRRLLQVAEVPETLQQFFDQLLSTEWSDKFVEQGELSQDIRRGRPQSALYR"
     gene            1875..2738
                     /locus_tag="PKW73_04650"
     CDS             1875..2738
                     /locus_tag="PKW73_04650"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNA72601.1"
                     /translation="MQEFLLAFFLSYVVHTLGLTIGYHRLLSHRSFHCSKAVEYFFVA
                     SGYLAFQTSPIWWATLHRAHHRYSDTAKDPHALMYGFRRCVYGWIFDEKYPDHIDPEV
                     MSKDLIKDPVYRFLDQGGNVQRGHILNGFLCFLFRVPILMIWGLPAALGSLAAGLIIQ
                     QVTLYFNLLSHIPKIGYRNFATTDDSVNNFWLALITMGEGWHNNHHGCPGSAKAGMRV
                     EEIDPAWYTIAFLRKIGLVSWANDTAFEQHCEKLARKALGEVLNISVVPSLVESPVEP
                     IPVPVSASSAK"
     gene            complement(2812..3132)
                     /locus_tag="PKW73_04655"
     CDS             complement(2812..3132)
                     /locus_tag="PKW73_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72602.1"
                     /translation="MAKTYTPEEKKQNFVNYFSYTVGIQVLVFTGCILANYLKVNLTV
                     LQAMYGLLMMTFSLYAAIPFCLSLYFLTWNFRSGKALGLFTASIVCLLVSATGCSLGN
                     KLLM"
     gene            complement(3134..>3824)
                     /locus_tag="PKW73_04660"
     CDS             complement(3134..>3824)
                     /locus_tag="PKW73_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72603.1"
                     /translation="VTLQKGLNDQEIGEIIDFALKEPCVRGVTFQPVQSAGRLETFNP
                     AKDRLTLSEVRAQILKQSPIFSDADILPVPCHPDCIAMAYALKLEDKVVPLSGLISPE
                     ELLKGRNTIQYEQDPLMKEKLFQLFSLNQAEKNTSHAVGDLLCCLPKLEFPTKLSYRN
                     IFRVIIIEFLDRYNFDVRSVKRSCVHIMHPDGRVIPFDTYNMFYRENLEVGQIARNGI
                     QKAEVKLDRIN"
BASE COUNT          879 a          927 c          988 g         1030 t
ORIGIN      
        1 ttgcgcaatc aatcgggggc tatggcttcc gtattactgc ttttgacctg cctcggttta
       61 ctgattgtgg gaagcatcgg cattgatgtc agtcatgctt tctatgtgcg cgctcaactg
      121 caaaccgtcg ccgacaattc ggccctcacc ggagcttact atctttgcag tttgatgccg
      181 ggacaagccg acgttaagcg ctccgaagaa atggctcgaa aaatggcggc tcgcagtata
      241 gttgacggtc ggtacttcga ggatgatggt gagaacagca ctctctccta cgcttccgag
      301 gtgaagtgcg gctccgcccc tcaagtttgt cggatctcca tgaccagacc ggtccccaca
      361 agtttcggtc gtctggttgg ttgccatact attccggttt ccgtgtcctc gcaggccggt
      421 gctttttgcg gtacgcgcac agttgctgcc aactggctga caaacctggc gatttctcac
      481 aaggtgcaga aggaaaacaa attagacctc gaccccagca gtgaagaaca caatgcctgg
      541 tttatccaag aatggagcgg gcagtcaaat ccggtcgtaa agatgggttc caccagtgtg
      601 agcaggggcc ctgtcaatct cgcttcttta gaaaagggca gggtttacaa cgtggccatt
      661 gtgaaaggag aaggggccaa aggtgaaaat cgattgccgc cttcatctga ggtgattggt
      721 accaccagta tcattgtcat tgcaaccgaa tccgctacca gcgctcgcat taaggttttg
      781 cccggtggtt tgatcaaggg gcagcccggc agctacctgg tgcccggcag taccggcgat
      841 gtgcagtttg tgcttcgaaa tggtcactgg cgcgtagcct tgatgaagta gccggcacat
      901 ggcacttgcc ggtggccagg cctctgttta agaccttgtg taaagcagcc tctttgttgg
      961 aagtgcgccc tgctcagcct tcgcggctgt gttgcaggtt taatgtgctt ccaatctcgg
     1021 atctccttta aaggctccac gaggttgcga cagccgtgat atgatagcga gtaactacaa
     1081 tttgtattca catgactaca aattgtaatt tgtgagtaaa cgagtaaaga tgaccagaga
     1141 taaggttaag accactaaca tcagcctgcg tatacccgag gaatatcgta agcagctgca
     1201 acttcaggca gacaaaaaag gagtgagttt caatgctcac ttgctgcgtg tcctggagtt
     1261 acatttgatg agctcaggat ttggtccgac ctccataaca tccgcctcgg gaagattgtt
     1321 tgagattaga agtgagctct acatagataa cgtcgatgag accagttttg ccttcttcat
     1381 cgatgagcct aagtttgaga aagagcgcgc ttattatgtc atcggcatcg gccgcactat
     1441 cttgcgtgac tggcagattc aggataagtt gaccgtttct aaggaagtcg gtctggctct
     1501 tctgaattac tacaaccgca agggtatgga tatagataag ttggtctgga atcaatatcc
     1561 gggcccggat gatgataatc gaagactgct ccaggttgct gaagttcctg aaacactcca
     1621 gcagttcttt gatcagttgc tgtctactga gtggtctgac aagtttgttg aacagggtga
     1681 attgagtcaa gacattcgca gaggccgtcc gcagtcggct ctctatcgct gattctccta
     1741 ttttattcgc gcttcctgtt catatttaag tggcaatgcc ggtgccagtg cttgaccgga
     1801 attgtcatgc tgactgattt atcgattagc cttttcaccc tgttttggcg tttgtcgccg
     1861 ggagatccaa gtacatgcaa gagttcctcc tcgctttctt cctttcttac gtggtccata
     1921 ctctcggatt gaccatcggc tatcacagac tcctctccca tcgctctttc cattgcagca
     1981 aggcggtgga gtatttcttc gtagcttccg gctatcttgc ctttcagact tcgccaatat
     2041 ggtgggccac cctgcaccgg gcccatcacc gctactccga tacggcaaaa gacccgcacg
     2101 ctctcatgta cggtttcaga cgttgcgttt acggctggat ctttgatgag aaatatcctg
     2161 atcatataga ccctgaagtg atgagcaaag acctcatcaa agatcctgtg tacagatttc
     2221 tggaccaggg cggcaatgtg caacgcggtc acatcctgaa cggttttctt tgtttcctct
     2281 ttcgcgtacc gatcttgatg atctggggac tgccggctgc tcttggtagt ttggcggcag
     2341 ggctgattat tcagcaagtt acgctttact tcaacttgct atcccatatt cccaaaatcg
     2401 gctaccgcaa ttttgccacc accgatgata gcgtcaataa tttctggctg gctctaatca
     2461 cgatgggcga aggctggcat aataatcacc acggatgccc gggctctgct aaagccggta
     2521 tgcgggttga ggagatagat ccggcctggt ataccattgc cttcttgcgc aagattggtc
     2581 tggtgagctg ggctaatgac acggcttttg agcagcactg tgagaagctg gctcgcaagg
     2641 ctctgggcga agttctcaat atttccgtgg ttccatctct ggtggagtcg ccggtggagc
     2701 ccattccagt tcctgtttca gcgagctctg ccaaataaaa actctcattt gatctgcaac
     2761 taaaaaaaat ccgacgggat tggcaaggct ttgtcaatcc cgcttcttaa tttacatcaa
     2821 gagcttgttg cccagagaac agcccgttgc cgatacgagc aggcaaacga tggaggcggt
     2881 gaaaagcccc agggcttttc cgcttctgaa gttccaggtg aggaagtaga gggagaggca
     2941 aaagggaatg gcggcataga ggctaaaagt catcatcagc aagccgtaca ttgcctgtaa
     3001 gaccgttaga ttgaccttca aatagttggc cagaatacaa cctgtaaaca ccagcacctg
     3061 aatgcccact gtgtaggaga agtaattgac gaagttctgc ttcttttctt ccggggtgta
     3121 ggttttcgcc atttcaattg atcctgtcca gttttacttc agctttttgt atgccgtttc
     3181 tggctatttg cccgacctct agattctctc ggtagaacat attgtaggtg tcgaaaggaa
     3241 tgacccgtcc atccgggtgc atgatatgta cgcaggagcg ttttactgat cttacatcga
     3301 aattgtagcg gtcaagaaat tcgataatta tcactcgaaa aatattgcgg taggacagtt
     3361 ttgtcggaaa ttccagcttc ggcaagcagc acaacaggtc gcccacggca tgagaggtgt
     3421 ttttctcggc ctgattgaga gaaaagagct ggaagagttt ttccttcata agcggatctt
     3481 gctcatattg aatggtattt cgccctttga gcaattcttc cggggaaatg agccccgaaa
     3541 gcggcacgac tttgtcttcc agtttgagag cgtaggccat ggctatacaa tcaggatggc
     3601 agggcactgg cagtatatcc gcatcggaga atatagggct ttgcttcagt atttgcgccc
     3661 gtacttccga gagtgtaagg cggtctttcg ccgggttgaa ggtctccagt cgtcccgccg
     3721 attgcaccgg ctgaaaggtt actccgcgaa cgcagggctc tttcagggcg aagtcgatga
     3781 tctcgcctat ttcttgatcg ttgagaccct tctgcagtgt tacc
//
LOCUS       DAOQNZ010000190         3823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11169_length_3823_cov_4.227442, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000190 DAOQNZ010000000
VERSION     DAOQNZ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11169_length_3823_cov_4.227442"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1440
                     /locus_tag="PKW73_04665"
     CDS             103..1440
                     /locus_tag="PKW73_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNA72604.1"
                     /translation="MNSKPNLEIRVGLFFVIAMFMLTIGWSWLKGISIAHPPQRFSVV
                     FSDIAGLNNNAPVNINGVRVGTVEKIELLQPDLAGKSPPSDLPKDDLSRQSATTQEEN
                     SRAMVNCQLKINTEAVTIPVGSTITIQTQGLVGAKYIEITLPEFIPGKEVRAISAGET
                     VRGQDPIRTELVLNRIATKLNDIVTSVGSEEVGVSLADALKHSGEAVSAFNQAAKKLD
                     KNMDRFEKAADSFTDTTKKVGEVAQEAKSAVKGASGFFHKGEEALGSMGSLARDVKVT
                     NSKVGKILDTPGLSADLKDTVNLAKQTADSIGVTIHELNGTLANQPLRQDLITMLTRL
                     NESVNSIERSVKTVDKLAEDQELRSDIKKVVSDARLAMDKVENLVNEPDFKSDLKGTI
                     ARVKDAAGNVDTAAQQLQQVLDKPAPLMHMMFGRPGKLAPKSQSAGNNTYKTR"
     gene            1466..2911
                     /gene="gatB"
                     /locus_tag="PKW73_04670"
     CDS             1466..2911
                     /gene="gatB"
                     /locus_tag="PKW73_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNA72605.1"
                     /translation="MALEAVIGLEVHAQLKTNTKIFCSCPTAFAASENEQVCPVCLGM
                     PGVLPVLNKKAVEFGIRTGLALNSKIAETCRFDRKNYFYADLPKGYQISQYEKPICLG
                     GYIEVNGRKVQIHRAHLEEDAGKLVHAGAAGLHGSDYSKVDFNRSSMPLLEIVSEPDL
                     TSPEEARLYLTELRNILRYIEVCDGNLEEGSFRCDANVSLRPVGQKELGTKTEIKNMN
                     SFRAVQRAIQSEIERQTALIEKGERIVQETRLWNEAEGKTHPMRSKEEAHDYRYFPEP
                     DLMPLTISRQWVEEVRKSLPELPAQRRQRYQSDLGLSSEDAAVLTETKELSDFFESSL
                     ALNAPAKAVANWLIGPATAYLKENKKEFCQTALTPQNMADLCQAVSSGKVSSTIAKQL
                     LLDLMSEGGDVAKLIESKGLAQVSDEGPIKAILQEVLDKSPTQLADYASGKVKLRQYF
                     FGEAMKALKGKANPQVINKLLDEMLPPVAAD"
     gene            2988..3671
                     /locus_tag="PKW73_04675"
     CDS             2988..3671
                     /locus_tag="PKW73_04675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNA72606.1"
                     /translation="MRFKVLTVLAALSLGSAAPAYNFKGYYAPAVLESPMVDNVTYTP
                     VGMGIVTSKAGFRVHPVTGRGDFHNGVDLSAKLNDRVYCLLDGVVTRVGWRGNLGVAV
                     EVYHPYPNVRTIVGHLNAYSVMPGMPIRRGRVIGYAGSTGRSTGVHVHYTVVKEDTHE
                     YIEPMQFINQVPQYVVALRSAQAQIAYSQNLKSFRKIDTKLIDVETTEDLPEGKKGKE
                     ADPPASMEP"
BASE COUNT         1007 a         1072 c         1004 g          740 t
ORIGIN      
        1 ccgaagaggg cgttagctca gaagaccctt atatgaaaca atttgctcat gccactcgtg
       61 aaggaccgat gttggcaggg gcgtttgagg gctgaacatt taatgaactc caaacccaat
      121 ttagaaatca gagtcggact cttcttcgtc atcgccatgt ttatgttgac gatcggttgg
      181 tcgtggctga aaggcatttc cattgcccac ccgccccaga gattcagcgt ggtgttcagc
      241 gacattgccg gtctcaacaa taatgcaccg gtcaacatca acggcgtacg agtgggaacg
      301 gtagaaaaaa tcgaactctt gcaaccggat ttggccggca aaagcccgcc ttcggatttg
      361 cccaaagacg acctgagccg gcagagtgct actactcaag aagaaaattc aagagccatg
      421 gtcaattgcc agctcaaaat caacaccgaa gcggtgacca ttcccgtggg ttcgacgatc
      481 accatccaga cccagggtct ggtgggtgcc aagtacattg aaatcaccct gcccgagttt
      541 atccccggta aggaagtgcg cgctatctcc gccggtgaga ccgtgcgcgg tcaggacccc
      601 attcgcaccg agcttgttct caacagaatc gccaccaagt tgaacgacat tgtcacctcg
      661 gtgggctccg aagaagtggg agtgagcctg gccgatgccc tcaagcattc gggagaagcg
      721 gtgagcgcct tcaaccaggc cgccaagaag cttgacaaga acatggatcg ctttgagaaa
      781 gcagccgaca gcttcaccga tactactaag aaggtagggg aagtggcaca ggaagccaag
      841 agcgccgtca aaggcgccag cggattcttc cacaaaggcg aggaagccct gggctccatg
      901 ggcagcctgg ctagagatgt aaaagtgacc aatagcaagg taggcaagat tcttgatacc
      961 ccggggcttt ctgccgacct caaggacacc gtcaatctgg ccaaacaaac cgccgattcc
     1021 ataggcgtga ccattcacga actgaacggt accctggcta atcagcctct gcgccaagac
     1081 ctgatcacca tgctcacccg cctcaatgaa agcgtcaact ccatcgaacg ctccgtcaaa
     1141 accgtagata agctggctga agaccaggag cttcgctccg acatcaaaaa ggtagtttcc
     1201 gatgccaggc tggccatgga taaggtagaa aacttggtta acgagccgga cttcaaatcg
     1261 gatctgaaag gtacaatcgc cagagtcaaa gatgccgcag gcaacgttga cacagccgca
     1321 cagcagcttc agcaagtgct cgacaaaccg gctccgctca tgcacatgat gtttggaaga
     1381 ccgggcaagc tagcccccaa gagccagagt gccggcaaca acacctacaa aacccgctag
     1441 agacgcagtc acaaggagcg aataaatggc actggaagcc gtcataggtc tggaggtgca
     1501 cgcgcagctc aagacgaata ccaagatttt ctgcagttgc cccaccgcct ttgctgcctc
     1561 tgaaaacgaa caagtttgcc ctgtttgcct gggcatgcct ggtgttctgc cggttctaaa
     1621 caagaaagca gtggaattcg ggattcgcac cggtctggct ttgaacagca aaatcgccga
     1681 gacctgccgc ttcgaccgca agaactattt ttatgccgac ttgcccaagg gctatcaaat
     1741 ttcccagtac gaaaaaccca tctgcctggg gggttatatt gaggtgaacg gcagaaaggt
     1801 acagatccac agagcccacc tggaagaaga cgccggcaaa ttggtacacg ccggtgctgc
     1861 cggtctgcac ggttccgact acagcaaagt agacttcaac cgctccagca tgccgcttct
     1921 ggaaattgtc agtgaaccgg atctgaccag ccccgaagaa gctcgcctct acctgacgga
     1981 actgcgcaac attctgcgct acatcgaagt atgcgacggc aacctggaag agggttcttt
     2041 ccgttgcgat gccaacgtct ccctcagacc ggtgggtcag aaggaacttg gcaccaagac
     2101 cgaaatcaaa aacatgaaca gctttcgggc agtgcaacgg gccattcaaa gtgaaattga
     2161 aaggcaaacc gccctcatcg aaaaaggcga gcgcatcgta caggaaacca gactctggaa
     2221 cgaggcggaa ggcaaaaccc atcccatgcg ctccaaggaa gaagcccacg actaccgcta
     2281 cttccccgaa cctgatctga tgcccctgac catcagccgc cagtgggtgg aagaggtgag
     2341 aaaatccctg cccgaactac cggcccagcg ccgacaacgc tatcaaagcg acctcggtct
     2401 ctcaagcgag gacgctgccg ttctcaccga aaccaaagaa ctgagcgatt tcttcgaaag
     2461 ctcactggcc cttaatgctc ccgctaaggc cgtggccaac tggttgattg gtcctgctac
     2521 cgcttacctg aaagagaaca agaaggaatt ctgccagacc gccctcacgc ctcaaaacat
     2581 ggcggatctc tgccaggcag tcagctccgg taaagtcagc tccaccatcg ccaagcaact
     2641 cctcctcgac ctcatgagcg aaggcggcga cgtcgctaag ctgattgaaa gcaagggact
     2701 ggcccaggtc tcggacgaag gcccgattaa ggcgattctg caggaagtac tggataaaag
     2761 tcccactcag ttggccgact atgcctccgg caaggtcaag ctcagacagt atttcttcgg
     2821 ggaagccatg aaagccctca agggcaaggc caatcctcag gtgataaata aactgctgga
     2881 cgaaatgctg ccgccggttg cggccgatta gactaaagca gcctgctttg aaaaaggcat
     2941 aatagtggta ttaatatagg aaaccctgga gtttcgccgt gctcgctatg cgattcaaag
     3001 ttttgactgt gcttgccgcc ctatccctgg gcagtgctgc tccagcctat aacttcaaag
     3061 gctattacgc gccggccgtt cttgagtcgc ccatggtcga caatgtcacc tatacgccgg
     3121 tgggcatggg tattgtcaca tccaaagccg gcttccgcgt acatcctgtt accgggcggg
     3181 gcgattttca caacggcgtc gatttgagcg ccaagttgaa cgatagagtt tactgtttgc
     3241 tggacggcgt cgtgaccagg gtaggctggc gaggcaacct gggagtagca gtggaggttt
     3301 accaccctta tcccaatgtt cgcaccattg tcggtcacct gaacgcctat tcggtcatgc
     3361 cgggcatgcc gattcgcagg ggcagggtga taggctatgc cggttccaca gggcgctcca
     3421 cgggcgtaca cgtgcactat acggtagtga aagaagacac ccacgagtac atcgagccca
     3481 tgcagttcat caaccaggta cctcagtatg tggtagctct gcgcagcgcc caggcccaaa
     3541 ttgcctactc gcaaaatctc aaaagtttcc gcaaaatcga caccaaattg attgatgtgg
     3601 aaaccaccga agatcttccg gaagggaaaa aaggcaaaga agcagacccc ccggccagca
     3661 tggaaccctg aatcccaggc tcagggcggc aactgcagcc gggtcaggat tctaatttat
     3721 aagatagaga ttgccgtcct ggctgccgat ccaaacaccg ccattggtga gagccggtga
     3781 actgactatg ggcctttgag tcttcagctt ccatttaata ggg
//
LOCUS       DAOQNZ010000191         3820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11187_length_3820_cov_3.844887, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000191 DAOQNZ010000000
VERSION     DAOQNZ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11187_length_3820_cov_3.844887"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1060)
                     /locus_tag="PKW73_04680"
     CDS             complement(23..1060)
                     /locus_tag="PKW73_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72607.1"
                     /translation="MFRFWKTAKAIANLFMLFLTWRWYYHGFGFEFSVGCLVLSGLWL
                     VLSFAQMGHLMRSYATALSRLQMLAPIWLGFALNTAAFFGTGDLAVRAFALAGFCAWG
                     YVYLRYRQERKNFFARGYGVLPRGIMVNPPAAMLPPGSILLMTGIPEYIGEAVGHGEI
                     VIPDAEGKKWAFSTYMKYGVVLNPLEEVLTRRARRGITVALAPSVPWTEEQCKMSFMV
                     ARGMLRQNKEWVVRERARQERLLAFLHLPEFVRRAWSKVFPVTGYDWFGLVLGRTKMG
                     RYTCVGSVQELLWRMGITLDRYFGTGLLGLGTTVLDPIMPARFFDLKCYRMVTEADHQ
                     AWLAAAQAAPK"
     gene            complement(1063..2184)
                     /locus_tag="PKW73_04685"
     CDS             complement(1063..2184)
                     /locus_tag="PKW73_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72608.1"
                     /translation="MRPVRKAFTFLSHCLFYGLATYFMLGHTVYGLDTAGRLLLLSGI
                     FLVVVYGLFNHILRSYGNIFSRLLAREAMLFGFPLSLAALLLQIGSGGGALIFSLAVF
                     SLAAWSFFAWRFVENRKRYEKQGAGPVPVDTCIKIPAAIVRPGDAIGTGGAMARLLRQ
                     GVAHTECVIPWQGKLMLFSSWFEEGAFLNEVERILKPDPKYPNYLLQRRPPLNEEQIE
                     LMEYVVRAMREQNLQYRERLTRQRERLPGWLKNYLDKKFPVTGYDWLGRYTGRRAPDR
                     WTCNVAYLEVMRRSCVDVVRLGHGAAGLRTGWLDFPNTEDLRGDPALHLVRNAELPAL
                     MADGPPRVNLNPDIPDFEAQVEALMAESINIFDRAKARS"
     gene            complement(2204..2944)
                     /locus_tag="PKW73_04690"
     CDS             complement(2204..2944)
                     /locus_tag="PKW73_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72609.1"
                     /translation="MAENNNKPEVKTYDRWAENYMLTGFALSFLLFFGIFLASESRDA
                     NTFWPAAGITLLVCAALPIAFNVLGRKGLARTIKCLTICLLFSTMVLGMFGCILGMGV
                     ADSYLQVDKVRAFAQEEFEMVDRGGDGLVDTGEVDAVLKEQDEISRGLKSLERIEQDL
                     QTLPVSKVAQELAVFGLLPAGAELKLLQLPPESLARVQAARQALYQIGKPAGNGFAAG
                     SAEFSDYKAKLERRYPLWIWVLREVGAL"
     gene            3476..>3820
                     /locus_tag="PKW73_04695"
     CDS             3476..>3820
                     /locus_tag="PKW73_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72610.1"
                     /translation="MQDDDLSFEQNYARLEAGLKPGFIKTDKTFGKNNLQGRGFLHED
                     AHDLAWLSGTVLCNPLVLALLSTILIGLGMSIFTSNNDKSALMTALAHFATRPAEGLA
                     IAGCFLASVLLLT"
BASE COUNT          940 a         1151 c         1001 g          728 t
ORIGIN      
        1 ctccttacct gtttcggcag ttttatttcg gcgccgcctg agcagccgca agccaggcct
       61 ggtgatcagc ttcggtcacc atgcggtagc acttcaggtc gaagaagcgc gccggcataa
      121 tcgggtccag caccgtggtg ccaagaccga gtagacccgt gccgaagtag cgatcgaggg
      181 tgatgcccat gcgccagagc agttcctgca ccgaaccgac acaggtataa cgccccatct
      241 tggtgcgccc cagcacaagc ccgaaccagt catagcctgt gacgggaaac accttactcc
      301 aggcccgtcg aacaaattcc ggtagatgca ggaaagccag cagtctctct tgcctggccc
      361 gctctcgcac cacccactct ttgttctgac gcagcatgcc cctggccacc atgaacgaca
      421 tcttgcattg ttcctccgtc cagggcacag aaggcgccag ggccaccgtg ataccgcggc
      481 gagcccgacg agtcagcact tcctccagag gattaagcac cacaccatat ttcatatagg
      541 tgctgaaggc ccacttcttg ccctctgcat ccggtatgac gatctcgccg tggccgacgg
      601 cttcgccgat atactccggt atgccggtca tgagtaagat ggagcccggc ggcaacatgg
      661 cagcgggcgg attgaccata atgccccggg gcagaacacc ataacccctg gcaaagaagt
      721 ttttccgctc ctgccggtag cgcagataga catagcccca ggcgcaaaag cctgccaggg
      781 caaaagccct gacggccaag tcaccggtgc cgaaaaatgc cgctgtgttg agagcgaagc
      841 ccagccagat cggcgccagc atctgcaggc gagacaacgc cgtagcataa cttctcatca
      901 gatgccccat ctgggcaaag gaaagaacca gccacaagcc gctcagcacc agacagccaa
      961 cggaaaactc aaacccgaag ccatggtagt accatctcca ggtgaggaag agcataaaca
     1021 agttggcgat tgccttcgcc gttttccaga aacgaaacat agtcagctcc tcgctttggc
     1081 gcggtcgaag atattgatac tttcagccat cagggcttcc acctgcgctt cgaaatcagg
     1141 aatatccggg ttgagattca cccggggagg accatccgcc atcagcgcag gcaactccgc
     1201 attgcgcacc agatgcagtg ccggatcgcc gcgcaagtct tcggtgttgg gaaagtccag
     1261 ccaaccggtg cgaaggccgg cagcaccgtg gccaaggcgc accacgtcca cgcaggaacg
     1321 tcgcatcacc tccagatagg ccacattgca ggtccagcga tcgggcgctc ttctgccggt
     1381 ataacggccg agccagtcat aaccggtgac cggaaacttc ttgtccagat agtttttcaa
     1441 ccagcctggc aagcgctcac gctgtcgtgt caaacgttcc cgatattgca ggttctgttc
     1501 ccgcatggcc cgcaccacat actccatcag ctcgatttgt tcctcattga ggggcggacg
     1561 gcgctgcagc aggtagttcg gatacttggg atcgggcttg agaatgcgtt ccacctcgtt
     1621 caagaacgcg ccttcctcaa accaggaaga gaagagcatc agcttgccct gccagggtat
     1681 gacgcattcc gtatgggcca ccccctgacg caatagccgg gccatggcgc cgccggtacc
     1741 gatggcgtca cccggtctga caatggctgc cgggatcttg atgcaggtat cgaccggcac
     1801 cggaccagcc ccctgctttt cataacgctt ccggttctcc acaaagcgcc aggcaaagaa
     1861 agaccaggcc gcgaggctga aaacagcaag tgagaaaatc agagcaccac cgccggagcc
     1921 aatctgcaaa agtagcgccg ccagcgagag tggaaaaccg aaaagcatgg cttcccgcgc
     1981 cagaagacgc gagaagatat tgccgtagct cctcagtatg tgattgaaaa ggccatagac
     2041 caccaccaga aagatgccgc tcagaagcag caaccgcccg gctgtgtcca gtccgtaaac
     2101 ggtgtgaccg agcatgaaat acgttgccag gccgtagaac aaacagtgcg acagaaatgt
     2161 gaaggctttc cgcaccggtc tcatgaacac ctcctgaagt tgatcagagg gcgccgactt
     2221 ccctcaggac ccagatccag aggggatagc ggcgttccag tttggcttta tagtcactga
     2281 attcggccga cccggcggca aaaccattac cggcgggctt gccgatctgg tagagggcct
     2341 gcctggcagc ctggacacga gcaaggctct caggcggcaa ttgcagaagt ttcagttccg
     2401 cccccgccgg caaaagtccg aacacagcca gctcctgcgc aactttgctg accggcaagg
     2461 tctgcaagtc ttgctcgatg cgttccagac ttttcagtcc gcgtgagatc tcgtcctgct
     2521 ccttgagcac cgcatccacc tcaccggtgt cgaccagacc gtcgccgcct ctgtccacca
     2581 tttcaaactc ctcctgagca aaggcgcgca ccttgtccac ctgcaggtaa gagtcggcca
     2641 cgcccatgcc cagaatgcag ccgaacatgc cgagcaccat ggtggaaaag agcaggcata
     2701 tggtcaaaca cttgatggtg cgagccaggc ccttccgacc caacacgttg aaagcgatag
     2761 gcagtgcggc acagaccaga agagtgatgc cggcggcggg ccagaacgta ttggcatccc
     2821 ggctttcgct ggccaggaag atgccgaaga aaagcagaaa ggacagggcg aagcctgtca
     2881 gcatgtagtt ttcagcccag cggtcataag tcttgacctc gggcttgttg ttgttttcag
     2941 ccatagagtt cctctttggg tgaagttgag aaaagctcca gccggcagtc accgacggag
     3001 catacagaga atattcaact tgaccgctca gccccggacg actgttcgtc tgaaaaatcc
     3061 gggcagaaag ttggtagaaa tatcggcttg agtctaaaag ttttgagctg agggtctgaa
     3121 gaagtagaaa ccaataacta acaaactcag atacttcaaa tagttaaaat ctatagttac
     3181 acaccccttg atagtccgta acaatattca aactacagaa ctggccgaac ttcagctaaa
     3241 tgacacggcg ctaagatggc ataccattaa tgcccgacca atgccgaacg gctagcctag
     3301 cgccgcgagg tttgaaaaac ctgagctact ttccgcccgc catataacaa ccacaaactc
     3361 tcgccactgc cacctccggc gacaccacca ccggcccgag gcaattttct cagtttcgaa
     3421 ctgttacgaa cgtggcatat tgccacgtaa caccgttgat ttgagctgcg caacaatgca
     3481 agacgacgat ctgagtttcg aacagaacta cgccagactt gaggccggct taaagccagg
     3541 tttcatcaag acggacaaaa ctttcggcaa gaacaattta cagggcaggg gctttctgca
     3601 cgaagatgcc catgatctgg cctggctgag cggcacagtc ctgtgtaatc cactggtgct
     3661 tgccctcctg tccacaattc ttatagggct tggaatgagc attttcacct ctaacaatga
     3721 caagtccgca ctgatgacgg cccttgccca ttttgcaacc agacccgcag aggggctggc
     3781 tatagccggc tgcttcctgg cttccgttct gttattgacc
//
LOCUS       DAOQNZ010000192         3807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11246_length_3807_cov_4.291311, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000192 DAOQNZ010000000
VERSION     DAOQNZ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11246_length_3807_cov_4.291311"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKW73_04700"
     CDS             complement(<1..230)
                     /locus_tag="PKW73_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNA72611.1"
                     /translation="MAKKNKILPLFPLPDVVLFPGSPLPLHIFEPRYRQMINTIMEGD
                     KTFGVLLYEPEHSHARVIGSSAVVTEMERLPDG"
     gene            complement(268..1335)
                     /locus_tag="PKW73_04705"
     CDS             complement(268..1335)
                     /locus_tag="PKW73_04705"
                     /inference="COORDINATES: protein motif:HMM:NF000689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNA72612.1"
                     /translation="MGRDYDAILFLSFGGPEGMDDVMPFLANVLRGKNVPESRMKEVA
                     HHYEMFGGVSPINAQNRKMIARLEEELERRQIKLPVYFGNRNWHPMLADTLKQMEADG
                     VKRALAFVTSAYSSYSGCRQYLEDIQRALSENQQNDRLHIDKLRVFFDHPLFLEANLE
                     CIEEALQQFDNLENLHLTFTAHSIPISMAQNCQYENQLLFVCRQICRRLQEKYNWPGG
                     EPQWQLVYQSRSGPKSQPWLEPDILEHIENLKNGGVSKILVHPVGFVSDHMEIIYDLD
                     TEARQLAESLQLQFVRSLSSGNSEHFGQLIGALIEERLKSKAGQECQIACLTGAPLPD
                     VCPADCCAYTPTRPVQTAGSH"
     gene            complement(1346..1576)
                     /locus_tag="PKW73_04710"
     CDS             complement(1346..1576)
                     /locus_tag="PKW73_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1541..1543),aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HNA72613.1"
                     /translation="MQELSITYCGSUGYKSRAVSLAAELQRLTGVTAKLHQSYGGVFE
                     IEVDGDLVFSKSKTGRFPMEGEVTSLLQAKVS"
     gene            complement(1636..2076)
                     /locus_tag="PKW73_04715"
     CDS             complement(1636..2076)
                     /locus_tag="PKW73_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNA72614.1"
                     /translation="METKVDKKLVIAALNQALEGELAAMVRYLHHSFIVLGPGRGPLV
                     TLFRTLAQDSMAHATMLGEKIVALGGHPSVAMDEVEEPGEQSLLQMLEEDIAVEEKHI
                     EMYERHLNQFQKDIVLRLMYEQIILEEVTHLEQLQLYVRHNDFN"
     gene            2224..3600
                     /locus_tag="PKW73_04720"
     CDS             2224..3600
                     /locus_tag="PKW73_04720"
                     /inference="COORDINATES: protein motif:HMM:NF023278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3370 family protein"
                     /protein_id="HNA72615.1"
                     /translation="MKIPTLLLASLSLMPPGLCAEGQESKPVNVNVNVPAQLRSLQGH
                     LDKSALFNSNSPEIVPEFSPAGILLSTLSPEGKASPDAHLNYRFHGRFGVFIHHINKQ
                     TRSQPEVLQLRLLAFNPGKKVASLHRLARSTYLSQPDAPFKPLPALSAHGGELYAGPG
                     DRVTLDFLTGKAFQVDGSPLLVGAGQWLLLDNLEVPVHGLSPALNGRSYLSWFKTSSP
                     LCLFLQASFQASWATAGSAEPFANALAKDQDLVRPRESSLKFPSFPGAKPIIYGRVSG
                     VSRGLTFTGRGHADIEAHKPLSLAFPISTLEGGTFGTKVQAAPMLRRYGDSAFAAHGN
                     YGLGYKIRLRVVNHDRLARTVKVSFNCPLKETGAAGKVRQLVYLEPPGKPVFYRGTVK
                     LTDYLSENSPFQGLFHLVLHRGERGEPVYKMNLEPFENRELEFELFYPPDATPPQVLL
                     LESETPEP"
     gene            complement(3597..>3807)
                     /locus_tag="PKW73_04725"
     CDS             complement(3597..>3807)
                     /locus_tag="PKW73_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72616.1"
                     /translation="NFMRGLKGNNPSITALLQENPSAGKDPPLYLRARLQQYQFDSPS
                     QILNSGIYWRIKPEAAKANYYNLGP"
BASE COUNT          819 a         1035 c          992 g          961 t
ORIGIN      
        1 ccatccggca ggcgttccat ttccgtaacg acggccgagg acccaatcac cctggcatgg
       61 gaatgttcag gctcgtaaag caaaacacca aaggtcttgt cgccttccat gatggtattg
      121 atcatctggc ggtagcgagg ctcgaaaata tgcagaggaa ggggactgcc cgggaagaga
      181 accacatcgg ggagtggaaa gaggggcaat attttgttct tcttagccac ggaacctcct
      241 cgaaagagtt tggtgttgca gtgggcgcta gtgagagccg gcagtctgca ccggtctggt
      301 cggtgtgtag gcgcagcagt cggcgggaca gacgtccggc agcggtgcac cggtcaggca
      361 cgctatctgg cattcttgcc ctgcctttga ttttagcctt tcttcgatga gagcaccaat
      421 caactgcccg aagtgttcac tgttgccgct gctcaggctg cgcacaaatt gaagctggag
      481 cgactcggca agttgtctgg cttccgtatc gaggtcataa atgatctcca tgtgatcgct
      541 gacaaagccg accggatgca caagaatctt gcttacgccg ccgtttttca ggttctctat
      601 gtgttccaat atgtcaggct ccagccaggg ctgggacttc ggaccgctgc ggctctggta
      661 gaccagttgc cattgcggct caccgcccgg ccagttgtat ttctcttgca ggcgtcggca
      721 aatctgccgg cagacaaaca gcaactgatt ttcgtactgg cagttctggg ccatggaaat
      781 tgggatgcta tgggcagtaa aggtcagatg cagattttcc aggttatcaa actgctggag
      841 cgcctcttcg atacactcca ggttcgcttc cagaaagaga ggatggtcga agaacaccct
      901 tagcttgtcg atgtgcagtc tgtcattttg ctgattttcg ctcagtgctc tttggatgtc
      961 ttccaggtac tggcgacaac ctgaatagga agagtaggcg gaggtgacaa aagcgagagc
     1021 ccttttgacg ccgtccgctt ccatttgttt gagggtgtcg gccagcatag gatgccagtt
     1081 tcggttgccg aaatagaccg gcagtttgat ctggcggcgt tccagttcct cttccaaacg
     1141 ggcgatcatc ttgcgattct gggcattgat gggagagact ccgccgaaca tctcataatg
     1201 atgagccact tctttcatgc gtgactcagg gacattctta cctctcaaga cgttggccag
     1261 gaatggcatg acatcatcca tgccctccgg tccaccgaaa gataagaaca aaatggcgtc
     1321 gtagtctcta cccacaataa cctcgtcagc ttacttttgc ttgtagaagg gaagtcactt
     1381 cgccctccat ggggaagcgt ccggtttttg atttagagaa gaccaggtct ccatctacct
     1441 caatttcaaa gactccacca tatgattgat gcagctttgc cgttactccc gtcaatcttt
     1501 gcagttcagc cgccaaactg acggctcgcg atttgtagcc tcaagaaccg cagtaagtaa
     1561 tgcttagttc ttgcacctgc aatctgtttt cgctcattgt ttttcctcgg ttaacagtac
     1621 tagttatctt gggtcttaat taaaatcgtt gtgtctgaca taaagctgca gttgctccag
     1681 gtgtgtaact tcttccagaa ttatctgttc gtacatgagc ctgagcacga tatctttctg
     1741 gaattgattg agatgccgtt cgtacatctc gatgtgcttt tcttccacgg cgatatcttc
     1801 ttccagcatc tgcaaaagcg attgctcacc aggttcttcc acttcatcca tggctacgga
     1861 agggtgcccg cccagggcta cgattttttc tcccagcatt gtggcatgag ccatggagtc
     1921 ttgagcgaga gtgcgaaaga gggtaaccag gggcccgcgg cccggaccaa gcacaataaa
     1981 agaatgatgc aaatatcgca ccatggccgc cagctcgccc tccagcgcct gattaagagc
     2041 ggcgattacc agtttcttat caactttcgt ttccataact tacttccgta gcatcattga
     2101 ataatttagc cttgccagct taagatgacc cttaatttta gtagatctta gccgccctgt
     2161 cctcaatctc ttgctcttta tggtctgggg cggcatacag cagagttacc agaagcgaaa
     2221 taaatgaaaa taccgaccct ccttctagcc tccctgtcac tgatgccgcc cgggctttgt
     2281 gccgaaggtc aggaaagcaa acctgtgaac gtaaacgtga acgtgcctgc gcaactgcgg
     2341 agcctccagg gacaccttga taagtcggct ctcttcaata gcaattcccc cgagatcgta
     2401 cctgagttct cgccggccgg aattttgctc tccaccctca gtcccgaggg caaagccagt
     2461 ccggatgccc atttgaacta tcgctttcat ggtcgctttg gcgttttcat acatcacatc
     2521 aacaagcaaa cccgctccca accggaggtt ctccaactga gattgctggc tttcaacccc
     2581 ggcaaaaaag tagccagcct tcaccgtctg gccaggagca cttatttaag tcagccggat
     2641 gcgcccttta agccactgcc tgccctctct gcccatggcg gcgaactcta tgccggtccc
     2701 ggcgaccgtg ttaccctgga cttcctcacg ggcaaagcct ttcaggtaga cggttctccc
     2761 ttgctggtgg gcgccggaca gtggcttctt ctggacaatc tggaagtgcc ggtgcatgga
     2821 ctctccccgg ccttgaacgg gcgctcctat ctgtcctggt ttaagaccag tagccctctt
     2881 tgtcttttcc tccaggcgag tttccaagct agctgggcga cggcgggtag tgccgagccc
     2941 ttcgccaatg ccctggctaa agaccaggat ctggtccgcc cccgcgagtc ttccctcaaa
     3001 ttcccgagct ttcccggcgc caagccgatc atctacggca gggtttcggg ggtttcccgg
     3061 ggtctgacct tcacaggacg cggtcatgcg gatattgagg ctcataagcc cctcagcctg
     3121 gcctttccca tctccacgct ggaaggaggc acctttggaa cgaaagtaca ggccgctccc
     3181 atgctcaggc gttacggcga ttcggccttt gccgcccatg gcaattacgg actcggctac
     3241 aagattagat tgagggtagt caatcacgat cggctggcca ggacggtgaa ggtcagtttc
     3301 aactgtcctt tgaaagagac cggtgcagcc ggtaaggtcc ggcagctggt ctatctggag
     3361 ccaccgggca aaccggtctt ttatagaggt acggtcaagt tgaccgacta cttgagcgag
     3421 aacagtccct tccaggggct tttccacctg gttctgcacc ggggcgagcg aggcgagccg
     3481 gtctataaaa tgaatcttga accctttgaa aaccgcgaac tggaatttga actcttctat
     3541 cctcccgatg ctactccgcc tcaggtgctg cttctggaaa gcgagacgcc tgagccttaa
     3601 ggtccgaggt tgtaataatt tgcttttgcc gcttccggct tgatgcgcca gtatatgccg
     3661 ctattgagaa tctgtgaggg tgaatcgaac tggtattgct gcagtctggc acgcagatat
     3721 agtggcggat cttttcccgc ggagggattt tcctgcagga gggctgtgat ggaaggattg
     3781 ttgcctttca gacctcgcat gaaattg
//
LOCUS       DAOQNZ010000193         3801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11267_length_3801_cov_4.608115, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000193 DAOQNZ010000000
VERSION     DAOQNZ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11267_length_3801_cov_4.608115"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKW73_04730"
     CDS             <1..451
                     /locus_tag="PKW73_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72617.1"
                     /translation="PVCGINLASLSKFSPARPATAEEKRRVETPSMQELVLDMVARQA
                     LSGADWQKVCRGAMEMYSISPNDVRGELFKRGYKFDEDGQPIKITGKARLHGLGENLV
                     PLKARKKGEDEISVKSRLKELLEEALLAADGPCREKLQEACRLLQSL"
     gene            560..2257
                     /gene="recN"
                     /locus_tag="PKW73_04735"
     CDS             560..2257
                     /gene="recN"
                     /locus_tag="PKW73_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNA72618.1"
                     /translation="MLKSIHIRDFALIENTLVEWTSGLNVLTGETGAGKSILMDALSA
                     VLGGKVAPSIIRPGKEKAAIEACFLAGPHVLAYLKQEELLADDESEELVVAREISKSG
                     SKVRVNGTLVNSAIVSELRGMLMTVHAQHEARTLMTPQSQLELLDGLGGDAHQKVVDK
                     VRTLYLRHKDLSAELNGLTMSEEDRLKSLDFANFQLKELTEAELLSDTEDEELEREIS
                     VLVNAIDLKSQALTAQELLTGGDITDGLSALDMVQKALLEVEKSLRFDPSLESLVESL
                     RSGLANIEEASLGLRRYGDSLDTDPGTLSELEARAALLSSVKRKYGPRLADARHKELE
                     LAELVEKLENSSLETEKIKDELTRLEKELLSQCAGLSDKRKKLAQSLSKQVESHLKDL
                     GMDKCRFEIAFQELPASGPNGTDRLEFLISPNPGQPPMPLSKIASGGELSRIMLAIKT
                     IFASADHVSTVIFDEIDTGLSGRVLNAVRDKLSVLSKSHQILCITHQPIVAAVADNYL
                     EVKKEHKKDETVVTVSRLDDEMRLRSLASMASGNASEESLTFARSLLEQANQARGQI"
     gene            2373..2444
                     /locus_tag="PKW73_04740"
     tRNA            2373..2444
                     /locus_tag="PKW73_04740"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2405..2407,aa:Gln,seq:ttg)
     gene            2533..3117
                     /locus_tag="PKW73_04745"
     CDS             2533..3117
                     /locus_tag="PKW73_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNA72619.1"
                     /translation="MEKIKLAVSKREAKTPNQLRREGFIPGTLYGAGVASENVQLCAK
                     EFSRLPQAAYSHMIELDYEGTTVNGLIRQVQRKSTKDFVYTVELYRVNLDKKLTVSVP
                     LKYVGASAAIHAGGQAVENYQEAELECLPSEIPDFVEVDMSMIENMEGAIHFGDIKLP
                     PTIKILNPLDEIVVKVVAPKAAPTPKEAAAAGKK"
     gene            complement(3181..>3801)
                     /locus_tag="PKW73_04750"
     CDS             complement(3181..>3801)
                     /locus_tag="PKW73_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72620.1"
                     /translation="ADFFQGKTLRSMLAEGLDSLLPLVECLKALTRLSKQEGFKYHGD
                     LKPDNILVASDNSILLIDPGHFDDLRSVHGNTAPMVVTTPAYYPGLLPNDLLAMGIIL
                     HETLTGYNPFAERRSLDEIENARLSDELRQRILMNEMTGNFFYSSLATLLPPSRGNPA
                     ITDSLESIILKGLGVKFQPDGTLDCQPGYGDFLAFAAALMNFIEGL"
BASE COUNT         1017 a         1031 c          978 g          775 t
ORIGIN      
        1 ccccgtttgc ggcatcaatt tagcctccct ctccaagttc tccccagccc gaccggctac
       61 ggcggaagaa aaacggcgag tggaaactcc ttccatgcag gagttggtac tggatatggt
      121 agcaaggcag gccctctcgg gtgcggactg gcaaaaggtc tgccgcggtg ccatggagat
      181 gtacagcatc tccccaaacg atgtgcgggg agaactcttc aagcgcggct acaagtttga
      241 cgaggacggt cagcccatca agattaccgg caaagccaga ctgcacgggc ttggtgaaaa
      301 tctggtgccg ctcaaagcca ggaaaaaagg cgaagacgaa atcagcgtca agagccggct
      361 gaaagaactg ctggaagaag ccctgcttgc tgcagatggt ccttgccgag aaaagctgca
      421 agaagcctgc cgacttctgc aaagcctata agcagggtaa acagggtctt tacgtccggc
      481 caaatcaagt tattatagga accagtcgct gtatttttgc cgactacagc cggttcccta
      541 ttgaccctat atacggtgca tgctcaaaag tatacacatc agagacttcg ctctcattga
      601 aaataccctg gtggaatgga ccagcggatt aaatgtgctc accggcgaaa ccggtgccgg
      661 caaatcaatt cttatggatg cgctcagtgc cgttctcggc ggcaaggtag cgccatccat
      721 tattcgtccc ggcaaagaga aggccgccat cgaagcctgc tttctggccg gtcctcatgt
      781 tctggcctat ctcaaacaag aagaactact agccgatgat gaaagcgaag aactggtagt
      841 cgcgcgtgaa ataagcaaga gcggcagtaa ggtgcgggta aacggcaccc tcgtgaacag
      901 cgccattgtc tctgagctga gaggcatgct catgaccgtg cacgcccaac acgaagcgcg
      961 caccctcatg acgcctcagt ctcaactgga gcttctggat gggctgggtg gtgatgccca
     1021 tcagaaagta gtagataaag ttcgcacgct ctacttgaga cacaaagatc tttcggccga
     1081 actgaatgga ctgaccatgt cggaagaaga ccgtctcaag tctctcgact tcgccaattt
     1141 tcagttgaaa gaactgaccg aagccgaact cctttccgat acggaagacg aagagctgga
     1201 gcgggagata agcgttctgg tcaatgccat agatcttaag agccaggccc tcaccgccca
     1261 ggaacttctc accggcggcg atatcacaga cggtctcagt gcgctggata tggtgcaaaa
     1321 agccctctta gaagttgaga aatccctgcg tttcgatccc agcctggagt cactggtaga
     1381 gagcctgcgc agcggtctgg ccaatatcga agaagccagc ctcggtctga ggcgctacgg
     1441 cgacagcctc gacaccgatc ccggtacctt gagcgagctg gaagccagag cggcgttatt
     1501 atcaagcgtc aaacgcaaat acgggccgcg cctggccgat gccagacata aagaactgga
     1561 actggccgag ctagtggaaa agcttgaaaa cagcagcctg gaaacggaaa aaatcaaaga
     1621 cgaacttact cgcctcgaaa aagaattact gagccagtgc gccggtctga gcgataagcg
     1681 taaaaagctg gcccagagcc tctcgaaaca ggtagagagt catctcaaag acctgggtat
     1741 ggataagtgc cgcttcgaaa tcgccttcca ggagctaccg gcaagcggac caaacggcac
     1801 ggaccgcctg gagtttctca tttctcccaa ccccgggcag ccacccatgc ctctttcgaa
     1861 aattgcctcg ggcggtgaat tgtcgcgtat catgctggcc atcaagacca tctttgcttc
     1921 ggccgatcac gtctccaccg tcatcttcga cgaaatcgac accggtctgt caggccgcgt
     1981 cttgaatgcc gtaagagaca agctgtccgt tctctccaaa tcccatcaaa ttctctgcat
     2041 cacccaccag cccatcgtag cggcagtggc cgacaactac ctggaagtca agaaagagca
     2101 caagaaagac gaaacggtgg tgacggtgtc ccggctggat gatgaaatgc gcctaagatc
     2161 tcttgcttcc atggccagcg gcaatgctag tgaagagtct ttaaccttcg ccaggtccct
     2221 gctggagcaa gccaatcaag cccgggggca aatctaagcg ctagtttcgt ttaacattgc
     2281 ggaatagaag cgcttattac attgacacga atgtatatca ttcaatagaa tgcatcgtta
     2341 ccttctggct tcagggcctt gagggtctct attggggcgt cgccaagtgg taaggcacct
     2401 ggttttggtc caggcactcc gaggttcgaa tccttgcgcc ccagttctgc ttttccggcc
     2461 aagcctgaag agccagagtt caatcacgtt atcgccaagt aaaaacacct ttgataggtt
     2521 aggaaaatag tcatggagaa aatcaaactg gcagtctcga aacgcgaggc taagacaccg
     2581 aaccagttga gacgcgaagg tttcatcccc ggtaccctct acggagccgg tgtggcatct
     2641 gagaacgtgc agctctgcgc caaagagttc agccgcttgc cgcaagctgc ctattcacac
     2701 atgatcgagc tcgattatga aggcaccaca gtcaatggac tgatccgcca ggtgcagcgc
     2761 aagtcaacca aagacttcgt ctacaccgtt gagctctaca gagtcaacct cgacaagaaa
     2821 cttacagttt ccgttcctct caaatatgtg ggcgccagtg ccgctattca tgccggcggt
     2881 caggccgtag aaaactacca ggaagcagaa ctggaatgtc tccccagtga aattcctgat
     2941 ttcgtagaag tagatatgtc tatgatcgaa aacatggaag gcgccatcca cttcggcgac
     3001 atcaaattgc ctcctaccat caagatcttg aatcccctcg atgaaatcgt ggtcaaggtc
     3061 gtagccccca aagctgctcc cacaccgaaa gaagcagccg cagccggcaa gaaataagcg
     3121 aaatcaaagg caaaagctaa gcaagagagc cgctccctgt ggggcggctt tcttttcgtc
     3181 tcacagcccc tcgatgaaat tcatcaaggc ggcggcaaaa gccaggaagt caccgtaacc
     3241 cggctgacaa tcgagggttc cgtccggctg aaacttcaca cccagcccct tcaaaatgat
     3301 gctttccaga gagtccgtga tggccggatt gcccctggag ggaggcaaga gagtagccag
     3361 ggaagaatag aaaaagtttc cggtcatctc gttcataaga atacgctgcc tgagttcatc
     3421 actgagcctg gcgttctcga tttcatcaag agaacggcgc tcggcaaagg gattgtaacc
     3481 agtcaaagtc tcatgcaaaa ttatacccat cgccagaaga tcgttgggca aaaggcctgg
     3541 ataataagca ggcgttgtca ccaccattgg tgcggtattg ccatgcacgc tacgcagatc
     3601 atcgaaatgc cccggatcaa tgagcaagat ggaattatct gaagcaacca ggatattatc
     3661 cggtttcaaa tctccgtgat atttaaagcc ttcctgcttg ctcagtcgag tcaaagcctt
     3721 caggcactcc accagcggca aaagcgaatc caagccctct gccagcattg atcggagcgt
     3781 cttgccctga aagaaatcag c
//
LOCUS       DAOQNZ010000194         3797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11295_length_3797_cov_3.550508, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000194 DAOQNZ010000000
VERSION     DAOQNZ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11295_length_3797_cov_3.550508"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKW73_04755"
     CDS             complement(<1..516)
                     /locus_tag="PKW73_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNA72621.1"
                     /translation="MSKPIVVIVGRPNVGKSTFFNRCIQEKHAIVDDTPGITRDRIYR
                     ECDWSGRDFILVDTGGILPDEGKSFEPMAKEVLEQVKQALLQADVIIFMVDGKEGITG
                     ADEEVANIIRRVKKPVILAVNKIDSLKETNAIMEFYGLGLGEPMPLSAMTGSGSVGDM
                     LDAIISHFPDNG"
     gene            complement(528..2549)
                     /locus_tag="PKW73_04760"
     CDS             complement(528..2549)
                     /locus_tag="PKW73_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72622.1"
                     /translation="MAPEEDKRQEEVELSLEVVDAAIKAVKGDDLDINPFTLGRHLGV
                     APELLFESRELMGRIIEARRKTQGDSATFSMDSELLLKVRGLEEELAKLAEVNQELYD
                     RGMAIEEQLLAAEAKVTDLEQEAETLAMQLQNSWHLGYNKGLADGQQKAGKEEAAPHT
                     TQENTGDNPAVGHTTVEHEVVEAEAPESDSQRDPQRDTLPPPQSEPISEAAPADTGNG
                     SRQSEEENVTDTGEFSSPEPSYNEKVFNAAHNSFVPAEGKDNYGNFSWREIETVYQYS
                     AVAGRPNINLYFEPFVQAPVMPEADEEDAVVIADRKQFQTGGMPAIKPEKPPQPETPD
                     RPNQNETQPIPGAAAFFQAQAKAEEAARQEEASRQKKKKTEERHIRNYMDMDDMNSGS
                     LTMGGGLYENPEAASTSPFARPINAGAPLADSLDEEKKPPELSTYDALNAVKIEDTHR
                     DELDLDKLDIFEGLEDIEELSQIEVIEDVVLPQEAADESEPAAKPAASQDLRELVKNR
                     IKQAEYETQTAEKAPPETVQTGGNNPRIDAESLKAGVRNKFVGGKASAGAPEQQAAPV
                     SAPRVVPPEIRKACMILGVRPEELTVKLVVDAWKREMSQPGVHPDTGGDTETAIYLNN
                     AKVELVKWIEAQTPKLGKKFGGQTGAKPQESGKPFIMGQKPPDKGQDKK"
     gene            2843..3160
                     /locus_tag="PKW73_04765"
     CDS             2843..3160
                     /locus_tag="PKW73_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNA72623.1"
                     /translation="MEKKTVYFKVTGRVQGVFYRQSTRYTAQNLGLTGFVRNLADGSV
                     EGLAHGDGQSLAVLMDYCRKGPPNAQVEQLVERFLEEAELSNWLANFSAAELPAAFLV
                     LRD"
     gene            complement(3183..>3797)
                     /locus_tag="PKW73_04770"
     CDS             complement(3183..>3797)
                     /locus_tag="PKW73_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA72624.1"
                     /translation="LIESGELWTNSLVTISEALAGFLLGTTIGAVLGLALWYSRLVAQ
                     ISRPYITALSAVPIFALSPIIISWFGIEITSKIMLAFLSTVCMALVQSYMGAMSVEKR
                     YLKLMQVVGAKRSQTFRLVVVPSSLIWVLNAMKINIGLALMGAFIGEFISSEQGLGCM
                     IVKASALYDMATVFVGIFALIVIALVFTFIIEKLENRLMVWKKL"
BASE COUNT          707 a         1027 c         1043 g         1020 t
ORIGIN      
        1 gccgttatcg ggaaagtgag aaatgatggc gtcgagcatg tcgcccactg aaccggagcc
       61 ggtcatggcg gaaagtggca tgggctcgcc cagacctaag ccataaaatt ccatgatggc
      121 attggtttct ttcagggagt cgattttgtt gactgccaga atcaccggtt tcttgacgcg
      181 ccggataata ttagctactt cctcatcggc gccggttatg ccttccttgc cgtccaccat
      241 gaagataatt acatcggcct gcagcaaggc ttgctttacc tgctccagta cttccttggc
      301 cattggttcg aagctcttgc cttcatccgg cagaataccg ccggtgtcga ccagaatgaa
      361 atcacgaccg gaccagtcac attcacggta gatgcgatcg cgggtgatac cgggggtatc
      421 atcgacgatg gcgtgcttct cttgaatgca acggttgaag aatgtggatt tgcccacatt
      481 ggggcgtcct actatgacga cgattggttt agacatgggt gaaaacttta cttcttatcc
      541 tggcctttat caggcggctt ctgccccatg ataaagggct tgcctgattc ttgcggctta
      601 gcgcctgttt gcccgccaaa cttcttcccg agcttgggtg tttgggcctc tatccattta
      661 accagctcaa ccttggcgtt gttcaggtaa atagccgtct cggtgtcgcc gccggtatcg
      721 gggtgaacgc ccggctgcga catctccctc ttccaggcgt cgacaaccaa tttgactgtc
      781 agttcttccg gtcttactcc caggatcatg caagccttgc gaatttccgg cggcaccact
      841 cgtggtgcgc tgacgggggc ggcttgctgt tccggggctc cggcggaggc tttccctccc
      901 acgaatttat tgcgcactcc tgctttcagg ctttcggcat cgatgcgggg gttgttgccg
      961 ccagtctgca cggtttccgg aggtgctttc tcggcggtct gcgtttcgta ttccgcttgc
     1021 ttgatgcgat tttttaccag ttctctcaag tcttgagagg cagccggctt ggcggccggt
     1081 tcgctttcat cagcggcttc ttgcggtagc acaacgtctt ctatcacttc tatctgagaa
     1141 agctcctcga tgtcttccag gccctcgaag atatccagct tatcgaggtc gagctcgtcg
     1201 cggtgggtat cctctatttt gacggcattg agcgcgtcgt atgtcgatag ttccggtggt
     1261 ttcttttcct cgtccaggga atcagccagc ggtgctccgg cattgatagg tcttgcgaag
     1321 ggagacgtgc ttgcggcttc ggggttctcg tatagccccc cgcccatggt cagactgccg
     1381 ctgttcatgt cgtccatgtc catatagttg cggatatggc gttcttctgt ttttttcttt
     1441 ttctgtctcg aggcttcttc ctgccgcgcc gcttcctcag ctttggcctg ggcctggaag
     1501 aaagcagcgg cgcccggtat gggttgtgtc tcgttctgat tgggtctatc cggtgtctcg
     1561 ggctggggcg gtttttccgg cttgatggcc ggcatgccgc cggtctggaa ctgtttgcga
     1621 tcggctatta caactgcatc ttcttcgtcc gcctccggca tcaccggtgc ttgcacgaaa
     1681 ggttcgaagt agaggttgat gttgggtcga ccggcaacgg cgctgtactg atagacggtt
     1741 tcaatttccc gccaggaaaa attgccgtaa ttgtctttgc cctcggcggg aacaaaggag
     1801 ttgtgggcgg cgttgaagac tttttcgttg taagagggct ccggcgacga aaactcaccg
     1861 gtatcggtaa cgttctcctc ttcactctgc ctggagccgt tgccggtgtc ggcaggcgcc
     1921 gcttccgaaa tcggctcact ttgcgggggc ggcaaagtat ctctttgggg atctctctgg
     1981 gagtcgcttt ccggggcttc cgcttccacc acttcatgtt cgactgttgt atgaccgacg
     2041 gcaggattat cgccggtgtt ttcctgcgtg gtgtgcgggg cggcctcttc cttaccggct
     2101 ttctgctgtc cgtccgccag acctttgtta tagcccaggt gccaggagtt ttgtagttgc
     2161 atggccagcg tctcggcttc ctgttccagg tcggtaacct tggcttctgc cgccagcagt
     2221 tgttcttcga tggccattcc gcggtcgtaa agttcctggt tgacttcggc cagcttggca
     2281 agctcctctt ccagacctct cactttcaaa agcagttcgc tgtccataga gaaagtggcg
     2341 ctgtcaccct gggtttttcg gcgcgcttca ataattctgc ccatcaattc gcggctttca
     2401 aagagcaatt ccggggccac gcccaggtgt ctgcccaggg tgaaggggtt gatgtccaag
     2461 tcatcaccct tgactgcctt gatggccgca tctacgactt ccaggctaag ttccacttct
     2521 tcctgtcgct tgtcttcttc tggcgccatg tagtgtcttg acttcctcag cctgtcagct
     2581 tttttcagcc tgactcgtgc ccttatacta atcaatcaga ttaaccggtc aaccggtcaa
     2641 ccggcgaacc tttgcctgat gaaccacctt tgctaaactc acagtgatgg tgccaggcaa
     2701 ttggtttctt cattgtagcc gcttgaccat ctttctatat tgattttatt aaagtctgag
     2761 tgttttagga aattcagacc atccccttta attcatggac gatcgctccg gttaccgaca
     2821 ggggtgggca agggaaggaa ttttggagaa aaagaccgtc tatttcaaag taaccgggcg
     2881 cgttcagggt gtcttttacc ggcagagcac tcgctatacc gcccagaatc ttggtcttac
     2941 cggctttgtg cgcaatttag cggacggctc tgtggagggt ctggcccatg gggacgggca
     3001 gtcccttgcc gtgcttatgg actactgccg caagggaccg cctaatgctc aggtcgagca
     3061 actggtggag cgttttctgg aagaggcgga gctttctaac tggctggcaa atttttccgc
     3121 cgccgaactg cccgctgcct ttcttgtctt gagagactga aggggcgctg actgctggag
     3181 ttttagagtt ttttccacac catcaagcgg ttttccagct tctcaataat gaaggtgaag
     3241 accagggcaa tcactatcag ggcgaagata cctacaaaga ccgtggccat atcgtaaaga
     3301 gcggaagcct tgacgatcat gcatcccagt ccctgctcgg aagagatgaa ttcgccaatg
     3361 aaggccccca tgagggccag accgatgttg attttcatgg cgttcaagac ccagatcaag
     3421 gaggagggca ccaccaccag tcggaaagtc tggctgcgtt tggctcccac cacctgcatc
     3481 agtttaaggt aacgcttttc caccgacatg gctcccatgt aactctgcac cagggccatg
     3541 cagacggtgg agagaaaggc cagcatgatt tttgaagtta tctcgatgcc gaaccaggaa
     3601 ataatgatcg gcgaaagcgc aaaaatcggt acggccgaaa gggcggttat ataagggcga
     3661 gaaatttgtg ccaccaggcg ggaataccac aatgccagtc cgagcactgc cccgatcgta
     3721 gtgccgagca ggaagccggc cagggcttcg ctgatggtca ccaggctgtt tgtccagagt
     3781 tcgcctgact ctatgag
//
LOCUS       DAOQNZ010000195         3792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11323_length_3792_cov_2.724913, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000195 DAOQNZ010000000
VERSION     DAOQNZ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11323_length_3792_cov_2.724913"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKW73_04775"
     CDS             complement(<1..231)
                     /locus_tag="PKW73_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNA72625.1"
                     /translation="MIKRLIIVNRGEIAVRIIRTCRDLGITSIAVYDESDVGSLHVLL
                     ADESVKLDSPKTFTDPKQLLELARLRKADAIHP"
     gene            complement(287..1822)
                     /locus_tag="PKW73_04780"
     CDS             complement(287..1822)
                     /locus_tag="PKW73_04780"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNA72626.1"
                     /translation="MKSYIDVLRAKKSAGRGTAAQAEQQHKKGRLTARERLDLLFDSG
                     TFQEIDSLVLPRYERYLGGKSSQFGDGVVTGFGLIAGRHAFAAAQDASVMGGSLGEMH
                     ANKIVKAMKMALSFGCPFIAMNDSGGARIQEGVDSLGGYARLFDANCEASGVIPQLSL
                     IMGPCAGGAVYSPALTDFVFMTANGYMFITGPDVVKAVMQEEVSQEHLGGGLVHSQES
                     GVSHFLAKDDTECIEQARELLSYLPSNNLDDPPYVKPNDDPDRRCPELEEIVPTDPSK
                     PYDVRTVIASVFDDGRFFEVHEEYAQNIVVGFARLNGHVVGVVANQPKVLAGTIDIKA
                     SVKGCHFIRICDAYNIPIVTLQDVPGFLPGTSQEYGGIIRNGARMIYAYSEATVPKLM
                     LILRKSYGGAYCVMSSKGLRGDLIYAWPSAELAVMGAAGAVNILFRKEIAEAADPVAK
                     RRDLEDDYKEKFSNSYVAASRGLIDDVIEPAESRRVLIRALELTLLKRERHTKRKHGV
                     SPM"
     gene            complement(2179..2394)
                     /locus_tag="PKW73_04785"
     CDS             complement(2179..2394)
                     /locus_tag="PKW73_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72627.1"
                     /translation="MNFKEHVSLASEQLPVLTGLSSCANLDQAFRFLESVGFAVAKME
                     LLSQDEFTLDLVFPFDDSNRCVVLSAS"
     gene            2505..3293
                     /locus_tag="PKW73_04790"
     CDS             2505..3293
                     /locus_tag="PKW73_04790"
                     /inference="COORDINATES: protein motif:HMM:NF021584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HNA72628.1"
                     /translation="MINKQRTAADLLTVSVLAAGVSLSFVLAGCSKPLEIFESESDVA
                     DTVHYPKNSEDLKLAAAEARRKWSFFEKEFIKKGSDKNISFLVKFPVSQGQTTEHLWI
                     NVTAINGPGISGTYDSDADFLPNIHRGDSAGILLKDMEDWMIDDGVTAWGGFSLQKLT
                     PKFIREIYSLNEEESDRSRRIEDLCKYWQKLADDAEKGIETEVSQQDMSYLSTLLRQQ
                     RMQKGNAGHEANDAELAKLLDLPEKMIQSSNKFWKLQQHTFKTR"
     gene            complement(3301..>3792)
                     /locus_tag="PKW73_04795"
     CDS             complement(3301..>3792)
                     /locus_tag="PKW73_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72629.1"
                     /translation="YLARLEIAESNRSYKALKKAKEKGANASEIATLQKSVEEFQNLA
                     RVHADQAVETKIRQVGSVHGYVENSWRTKGQVLYALHLYPEAQEAFTKALSIAEKLKI
                     PDAEAGRCLNIAKCLEAQGKFEEAKTFYERSIRVSKELFGPQHQEVKEVVDEYNEFMK
                     RHY"
BASE COUNT          943 a          996 c          949 g          904 t
ORIGIN      
        1 ggggtgaatg gcgtctgcct ttctcagcct ggccagttcc agcagttgct tggggtcggt
       61 gaaggtcttg ggtgaatcca gcttgactga ctcgtctgcc agcaacacgt gcagggaacc
      121 cacatcagac tcgtcgtaca cggctataga tgtaatcccc aggtctctac aggtgcgaat
      181 gatcctgact gcaatttcgc cccggtttac tattattagt ctcttgatca tttccctgcc
      241 aaattatgct gaaggctgtc ctactgtcgt gaagcgccag gcgccctcac attggtgaaa
      301 cgccgtgttt gcgcttggta tggcgctctc gtttgagcag ggtcaattcc agggcgcgaa
      361 taagaacccg acgcgattcg gccggctcga ttacgtcatc aatgagaccg cgagaggcag
      421 ccacataact gttgctgaac ttctctttgt agtcgtcttc cagatcgcgg cgcttagcca
      481 ccgggtcggc agcctcggca atttctttgc ggaagagaat gttcaccgct ccggcggccc
      541 ccatcacggc aagctcggca ctgggccagg cgtaaatgag gtcgccgcgc aagcccttac
      601 tgctcatgac gcagtaggcg ccgccgtaac tcttgcgcaa tatgagcatc aacttgggaa
      661 cggtggcttc gctataggcg tagatcatgc gggcgccatt acggatgata ccgccgtatt
      721 cctggcttgt tcccggtagg aagcccggca catcttgcag agtaacaatc ggaatattgt
      781 aagcatcgca gatgcgaatg aagtggcacc ccttaactga tgcttttatg tcgatggtgc
      841 ctgccagcac cttgggctgg tttgccacca ctcccaccac gtgcccgttc agtctggcaa
      901 aaccgacaac tatattctgg gcatactctt cgtgcacttc aaagaatcta ccgtcatcaa
      961 agacactggc aatgacggtg cgaacgtcat aaggcttgga gggatcggtg ggtacgattt
     1021 cttccagctc cgggcagcgg cggtcgggat catcgttggg cttcacgtaa ggtggatcat
     1081 cgagattatt cgaaggcaag tagctgagca gttcccttgc ctgttcgatg cactcggtat
     1141 cgtccttagc caggaaatgg ctgacaccgc tttcctgact gtgcaccaga ccaccaccca
     1201 agtgctcctg gcttacttct tcctgcatca cagccttaac tacatcagga ccggtgatga
     1261 acatgtagcc gttggcggtc ataaaaacga agtcggtcag tgccggcgag taaacggcgc
     1321 cgccggcgca aggtcccata atcagcgaca actgcggaat tacaccggag gcttcgcagt
     1381 tggcgtcaaa gaggcgggcg taaccgccaa gactatccac gccttcctga attctggcgc
     1441 caccggagtc gttcatggcg atgaaggggc agccgaagga aagggccatc ttcattgctt
     1501 tcacgatttt attagcgtgc atctcgccaa gggaaccacc cataaccgat gcgtcttgcg
     1561 cagcggcgaa ggcatggcga ccggcaatca gcccaaagcc ggtgacgact ccgtctccaa
     1621 actgcgaaga ttttccaccc aggtaacgct cgtagcgcgg cagcacaaga ctatcgatct
     1681 cttgaaacgt gccgctgtcg aagagcagat ctaagcgctc tctggctgtc agacgtccct
     1741 tcttgtgttg ttgctccgct tgtgcggcag tgcctcgtcc ggcactcttc ttagctctaa
     1801 ggacgtcaat ataggatttc atgaatgaaa aagacactcg caatttacta acgccgcgaa
     1861 ttatagcgct tttcctgatg aacattgctg ccacagccag ggtcttcaat tttggtctgt
     1921 catatgtagt ggtggcaaaa tacttacgtt tctgaaacta gccatccttt ccgaaaacgc
     1981 atttaatatc gaatcccgct tggcagccgg gtctctagct cagtcagttt tgaagcggca
     2041 tgcagcatag ccaagtatcc ttgggccgtc ttgcagttca gaaacctggg cagtccaacc
     2101 catctgcagg tgccagttca atagttcatc attcgtcggt ttcgcatcga agacggcgac
     2161 cgccgtcaaa tgccccactc aactggcgga aagcaccaca caccgattag aatcatcgaa
     2221 tggaaaaacc agatcgaggg taaactcatc ctgggatagc agttccattt tggccacagc
     2281 aaagcccaca ctctctagaa agcgaaaggc ttgatcgagg ttggcacagg aactcagccc
     2341 tgtgagaacg ggtaattgct cggaagccaa gcttacatgt tctttgaagt tcatagtact
     2401 ctccctctgc ccttctacca tttaagcagt gagcagcaaa ggcgtacatt cccgaattcg
     2461 ggtaaaattg aaaatttgga aaggtataaa cgagtttccc aaagttgatc aataaacaaa
     2521 gaactgccgc ggacttactc acagttagcg ttctcgctgc cggtgtgtct ctcagttttg
     2581 tacttgcagg ctgctccaag ccattagaga tattcgagag tgaatctgac gtggccgata
     2641 cggtccacta tccgaaaaac tctgaagacc tgaaattggc agcggcagaa gccagacgaa
     2701 agtggtcttt ttttgaaaag gagttcatca aaaaaggatc cgacaaaaat attagtttcc
     2761 tggtcaaatt tccagtcagc caggggcaga caaccgagca cctatggatc aatgtcacgg
     2821 cgataaacgg accaggcata agcggtactt atgacagtga cgctgacttt cttcctaaca
     2881 ttcacagagg agatagcgcc ggtattctcc tgaaagacat ggaagactgg atgatcgacg
     2941 atggtgtgac ggcctggggc ggattcagcc tacagaaatt gaccccaaaa tttataaggg
     3001 agatttactc tcttaacgaa gaagaatccg ataggagccg cagaattgaa gacctttgca
     3061 agtactggca gaagctagcc gatgacgccg aaaaaggcat agagacggag gttagccagc
     3121 aagatatgag ttacttaagc actcttttgc gtcaacaaag aatgcagaaa ggcaacgccg
     3181 gacacgaggc taatgatgct gaactggcta aacttctgga cctaccagaa aagatgattc
     3241 aatcaagcaa caaattctgg aaactgcaac aacacacatt caaaacacgt tgaggcactg
     3301 ctagtagtgg cgcttcataa actcgttgta ctcatcaact acctctttca cttcttgatg
     3361 ctgcgggccg aaaagctctt tggatactct tatggagcgc tcatagaagg tcttcgcttc
     3421 ttcaaacttt ccctgggctt ccaggcattt ggcaatattc agacagcgcc ctgcttccgc
     3481 atcaggaatc ttcaattttt cggcgatgga gagagccttg gtgaacgcct cttgcgcctc
     3541 tggataaaga tgcaaggcat aaagtacctg ccccttggta cgccaggagt tttctacata
     3601 tccatgcacg gatcctactt gccgaatctt ggtctcaaca gcctgatcgg catggactct
     3661 ggcgagattt tgaaattctt ccaccgactt ctgaagagta gctatttcag aagcattagc
     3721 ccccttttcc ttcgccttct tgagcgcctt gtagctacgg ttagactctg cgatttccaa
     3781 gcgggccagg ta
//
LOCUS       DAOQNZ010000196         3790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11334_length_3790_cov_3.732262, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000196 DAOQNZ010000000
VERSION     DAOQNZ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11334_length_3790_cov_3.732262"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /gene="dnaX"
                     /locus_tag="PKW73_04800"
     CDS             complement(<1..1238)
                     /gene="dnaX"
                     /locus_tag="PKW73_04800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNA72630.1"
                     /translation="MEPYLPLYLRHRPQTLTQLVGQRSVVKTLTNAIDNDRIAHAYLF
                     TGPRGTGKTSSARILAKSLNCEKGPTAEPCMVCPMCVEIKQGISPAVLEIDAASNNSV
                     DDARVLIERAPLVAQGGRFKLYIIDECHMLTKEAFNALLKTIEEPPPKVIFVLATTEE
                     HKVPPTIISRCQRLMFRLVNHEDLTKHLRNVADQEDIHIEDSALELIARRSGGGLRDA
                     MGLLDQASLLATKEKPVAVADLLTLLGAIDEDVLLDISKGMRDRDGEQVLTAVHGLLS
                     QGREPSLVALELSKHFLNMTKALHLGKKKSSGGDSPLAQVISGSAGYIEAITDLAPAF
                     EGYELTQMVEHLDRLEQTLRRSTQPALSLEVGLLSLCYRQEINLVKDLVKRIEHLEAL
                     MTDGGGVITPSHRSSSPAQAP"
     gene            complement(1361..2386)
                     /locus_tag="PKW73_04805"
     CDS             complement(1361..2386)
                     /locus_tag="PKW73_04805"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNA72631.1"
                     /translation="MQAIFSDIEKLRKLPKAELHLHIEGTFEPELIFELAAKNRVALN
                     YKSVEELRQAYQFSDLQSFLNIYYAGMSVLKDEHDFEKLAESYFERAAADGVVHAELF
                     FDPQAHTGRGIKFDTVINGLHRACKNSASKYGLTSALILCFLRDLDSESAMATLEEAL
                     KQREKFIAVGLDSAEVGNPPSKFEKVFARARAEGLLTVAHAGEEGPPPYVWEALDLLK
                     VSRIDHGVRSLEDEKLTKRLKAEEIPLTVCPLSNVKLKVFKDLKEHNLKKMLDLGLKA
                     TINSDDPAYFGGYVLENYNQCRLALSLSDEDLIQLAKNSITASFLPENEKRKHLQVIE
                     NQFNLTT"
     gene            complement(2455..3330)
                     /locus_tag="PKW73_04810"
     CDS             complement(2455..3330)
                     /locus_tag="PKW73_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72632.1"
                     /translation="MTQIEQWASGDGKQDKILAFQGGENHGLWMKDLANFQGNGERLE
                     PQFKRPQDNLVLVQNTADEPEEETEAHAPESMLALLRAEDPANLGKGLRSMQGLAEEF
                     ATAKDKPKALNQLREGFEGAVKQTDTDFEVVKANFEAERIKLKPEMEPRLKAMEQAGK
                     EMGEAMGKLPKEEQERMKWAMAGYKNGSNSQEFDQAIAKEIGKHPGLLPALEAGLKAS
                     NDLAPYMEKAQALKQGMENAMAERIAGRQAYSEVLKEGGDDKRAQRMDLEARAIMMGI
                     PLENIDAIEKRQQGK"
     gene            complement(3459..>3790)
                     /locus_tag="PKW73_04815"
     CDS             complement(3459..>3790)
                     /locus_tag="PKW73_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72633.1"
                     /translation="SAQGNVALQAYLRSGKLEALRVYNADYLPASLGVSIESELSTMK
                     SKFGEPSFVLDEPPANHTLNGKPNKGTAATAKNYVYPLSQVGFQLARPSAGASPQVMS
                     VLFFRYL"
BASE COUNT          736 a          975 c         1054 g         1025 t
ORIGIN      
        1 ggagcttggg ctggtgaact gctgcggtgt gatggcgtga tgacaccgcc gccatcggtc
       61 atcagtgctt ccagatgttc gatgcgtttg accagatcct tgacgagatt gatttcctgt
      121 ctgtagcaga gggaaagcag tcctacttcc agagagaggg ctggttgggt ggagcggcgc
      181 aaggtctgtt ccagtcgatc caggtgttcc accatctggg tcagttcata gccctcaaag
      241 gcgggagcca ggtcggtaat ggcttcgata taaccggccg aaccggagat cacctgggcc
      301 agtggcgaat cgccgccact acttttcttc ttgcccagat gcagagcttt ggtcatgttg
      361 agaaagtgct tgctcaattc cagtgccacc agggatggtt cgcgcccctg gctcaagagt
      421 ccgtgcacgg cggtgagaac ctgctctcca tcacgatctc tcatgccttt gctaatatcg
      481 agaagcacgt cttcgtcgat ggcacccaga agagtaagca agtcggccac ggccaccggt
      541 ttttctttgg tggcaagcag ggaggcctgg tctaagaggc ccatggcgtc tctcagtccg
      601 cccccggagc ggcgggctat cagttccagg gcgctatctt cgatatgaat atcttcctgg
      661 tcggccacat tgcgaagatg cttggtgagg tcttcgtgat tgaccaggcg gaacatgagg
      721 cgctggcagc gactgatgat agtcggcgga actttatgtt cttccgtggt ggccagtaca
      781 aaaatcacct tgggcggcgg ctcttcaatg gtcttcagga gagcattgaa agcttccttg
      841 gtgagcatgt ggcattcgtc tatgatgtag agcttgaagc gcccgccctg ggccacaagt
      901 ggtgctcttt cgatcagtac acgggcgtcg tccacggaat tattggaagc ggcgtcgatt
      961 tccagtaccg ccggcgatat accttgtttg atttccacgc acatggggca aaccatgcag
     1021 ggctcagcgg tgggaccctt ttcgcagttg agggatttag ccaggatgcg ggcgctggag
     1081 gttttaccgg tgccgcgggg acctgtaaat agataagcat gagcaatgcg atcgttgtcg
     1141 atggcattgg ttaatgtctt gactacggag cgctgaccca ccagttgtgt gagggtctgg
     1201 gggcgatggc gcaagtaaag tggtaggtat ggctccaagg ttcttcagcc ttatttattg
     1261 caacaggccg gatgcttggc ctgagcagga aagaagcggc gtttgaattt gccaagctta
     1321 ttttaaccga tcaactgctc cgggctctaa atcttgcttg tcaggttgtc agattgaatt
     1381 ggttctctat gacctggagg tgcttgcgct tttcattttc aggaaggaag gaggctgtaa
     1441 tggagttttt cgccagttga atgagatctt cgtcgctcaa tgagagggcc agtcggcact
     1501 ggttgtagtt ttccagcaca tagccgccga agtaagccgg gtcgtcggaa ttgattgtgg
     1561 ctttaagtcc gaggtcgagc atcttcttga gattgtgctc tttaagatct ttgaaaacct
     1621 tcagttttac attcgatagg gggcagaccg taaggggaat ctcttccgcc ttcagccttt
     1681 tggtaagttt ctcgtcctcc aggcttctga cgccgtggtc gatacggctc actttcagca
     1741 aatcgagggc ttcccagaca tagggcggcg gtccttcttc gccggcgtgg gccactgtca
     1801 ggagtccttc ggcgcgggct cgggcaaaga ctttctcgaa cttgctcggg ggattgccga
     1861 cttccgccga atcgagaccg acggcgataa acttctctct ctgcttgagc gcttcctcca
     1921 acgtggccat ggcactttcg ctgtctaggt ctcgcaagaa gcagagtatc agtgcactgg
     1981 tcaggccata cttgctggcg gaatttttgc aggcgcggtg aagcccgttt atgacggtat
     2041 cgaatttgat gcctctacca gtatgggcct ggggatcgaa aaagagttcg gcatgaacaa
     2101 cgccgtctgc cgccgctctc tcaaaataac tttcggccaa cttttcaaag tcgtgctcgt
     2161 ctttgagtac ggacataccg gcatagtaga tattcaagaa ggattgcaga tcgctgaact
     2221 ggtaggcctg cctcagttct tccacacttt tgtagttgag ggccacgcgg ttttttgccg
     2281 ccagctcaaa tatcagctcc ggttcgaagg tgccctcaat gtgcaggtgc aactccgctt
     2341 tgggaagttt ccttagcttc tcgatatcac tgaaaattgc ttgcataaac tccgttaccc
     2401 tggcacgggg agaccgatgg gttccccagc tgatgcacaa gatttgagtc tacactattt
     2461 cccttgctgg cgtttctcaa tagcgtcgat attttccagg ggaataccca tcattatggc
     2521 ccgcgcttcc agatccatgc gttgtgccct tttatcgtct ccgccttcct tcagcacttc
     2581 actgtaagcc tggcgcccgg caattctttc agccatggca ttttccatgc cctgcttgag
     2641 agcctgggcc ttttccatat aaggagccag gtcattgctt gctttcagtc cggcttccag
     2701 ggccggtaag agtcccggat gtttgcctat ttctttggcg atggcctggt cgaattcctg
     2761 tgaattggat ccgtttttgt aaccggccat ggcccatttc atgcgctctt gctcttcctt
     2821 gggcagtttg cccatggcct cgcccatctc tttgccggcc tgttccatgg ccttgagtcg
     2881 gggttccatt tccggtttca gtttaattct ctcggcttcg aagttggctt tcactacttc
     2941 gaaatccgta tcggtctgct tcacggctcc ttcgaagcct tccctgagct ggttgagagc
     3001 cttgggttta tccttggcgg tggcgaattc ctctgccagt ccttgcattg atctgagacc
     3061 tttgccgaga ttggccgggt cttcagcacg caagagagca agcatgctct ccggtgcatg
     3121 ggcttccgtt tcttcttccg gttcatcggc cgtgttctgc accagaacca ggttatcctg
     3181 cgggcgtttg aattgcggtt ctaatctttc gccattgccc tggaaattag ccaggtcttt
     3241 catccataaa ccgtgattct ctccgccttg aaatgccagt atcttgtctt gcttcccatc
     3301 gccggatgcc cactgttcga tttgcgtcat caacaacacc tgtgcctgaa ttagattatc
     3361 tacgggggta tttacgccag ttctcgggga gcgtaacacc aaagccgaca gttgtcaggt
     3421 gggcgaaata cgaagaaggc ggaatttaag gaaggctctt aaaggtagcg gaaaaatagt
     3481 acgctcatca cctgcgggct ggcgccggct gaaggtcttg ccagttggaa gccgacctgg
     3541 ctgaggggat agacatagtt tttagcggtc gctgctgtcc ctttatttgg cttgccgtta
     3601 agggtgtgat tggccggcgg ctcatccaga acaaaagaag gttcgccgaa tttgcttttc
     3661 atggtgctca gctcgctttc tatggagaca cccaggctgg ccggcaggta gtcggcatta
     3721 tagactctca gggcttccag ttttccgctg cgaagatagg cttgcagagc cacattaccc
     3781 tgagcgctac
//
LOCUS       DAOQNZ010000197         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11406_length_3779_cov_3.837003, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000197 DAOQNZ010000000
VERSION     DAOQNZ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11406_length_3779_cov_3.837003"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..895
                     /locus_tag="PKW73_04820"
     CDS             221..895
                     /locus_tag="PKW73_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72634.1"
                     /translation="MAPSEENQKVRKSNFQFFTSLLVTEKEWARVRKPVISAFILFHG
                     MLNFCYMFNHHPYTQALNYFFHNYYFFLGLDQDFGVFAPVPRNSNPHLVAIVRYKDGT
                     TRIWNSPRMERLGFLEKIPKERYRKFFDDNCVWDKVPETWPDIARYVARASMVEKSNP
                     PETVSLIRFSSKIPPPPMPDDLPPPQGQDRKKQEKTKWEANPPHYESKLLITLKIHPQ
                     DLEGVK"
     gene            896..1852
                     /locus_tag="PKW73_04825"
     CDS             896..1852
                     /locus_tag="PKW73_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HNA72635.1"
                     /translation="MSIKTLFKLWDEFWFTETSPLAVAVFRILMGIIMILFVLLLTQD
                     MLVWFGQYGLISNQTERNFAQVVGLNVLNLARDNDTFLLAFFVVFFAASLCMTIGYKT
                     RLSVLVVFLCLNSLYHRNAFLFNSGDTYMRVMSFWLFFSASGNALSIDHLLKLKSKRA
                     PESPEEAEKFNYKCVSKWPVRLLQLQLALVYFHTFVAKFWGDTWVNGTSVYYSSRIED
                     LVRVKLPYVFDNLWTCQLLTWGTLVIELALFTLIWVKEFRYYVIALAIVFHLTIDLHM
                     NIPLFEWIMMFSYVLFIDNQDLQKGLLKIKQKFPALFPVLKD"
     gene            complement(1842..2582)
                     /locus_tag="PKW73_04830"
     CDS             complement(1842..2582)
                     /locus_tag="PKW73_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72636.1"
                     /translation="MATVGKIDAKRKLFARVFAWMASLAFIASISTTLYMVSYGGLVA
                     YDNETLQSERLKLAETRAREEVRFNEESGKSALEKARAMKELAIYYQESRNFAAARDT
                     LLSAEKLVALEYDDVDHLLLRIDLKRQAALVMCDCTLFQEAEPLFRQAGRIVSRLEAI
                     DKDAAGIAKAKLLNDEGVMYYLWAGSTRELAERGQRYEVAADCFLKAGKILTRSSKEN
                     ASLKVAIADNLRQLRDDQKYEKLTYLSP"
     gene            2759..3517
                     /locus_tag="PKW73_04835"
     CDS             2759..3517
                     /locus_tag="PKW73_04835"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72637.1"
                     /translation="MSNGHSLNLKMMLAVLAAFLVTGSASAQNGAPTPGAAKDSHKKE
                     EAKTTASSEQNPALKPVNEKILPLLNNGYELLSKGQYDKATQVLEKAVKADGESISAR
                     RYLAFAQVRNGDNLKALSNLQALSRLVTPGALDWYVFGEAYMGAGGLQHAKSCFNQAL
                     TFSPDYAAARGGLVKSQIRLKEFDEALSTVQDGINKTRDPQIRKYFSALYQNAAAQKE
                     AARRALITSPVPESSTTATQEETGPVLIKPSNDS"
BASE COUNT          991 a         1003 c          871 g          914 t
ORIGIN      
        1 cctggcggaa actgagtgga gaaagtgcgt caacatttcc gccggcgcgc ccctcaagtt
       61 aattccaaag gaatggcgga aaatttcttt gcaacaattg agtattgccc tcaaaggcca
      121 gggtaagacg gaacaagcga agcaagttga cgagcaagcc aaaaagatct agctaaggcc
      181 ggcagttgct tcagatttcc ggctaaaagg ggaataaacc ttggccccat ctgaggaaaa
      241 ccaaaaagta cgcaagagta actttcaatt tttcaccagc cttctggtaa cagagaagga
      301 atgggcgaga gttaggaagc ctgtcatttc ggctttcata ctttttcacg gtatgctcaa
      361 cttctgctac atgttcaatc accatcctta cacccaggca ctcaactatt tttttcacaa
      421 ctactatttc ttcttaggtc tcgatcaaga tttcggagtc tttgccccgg tacccagaaa
      481 cagtaatccg cacttggtgg ccattgtgcg ctacaaagac ggcacaacca gaatctggaa
      541 ctcaccacgc atggaaagac ttggttttct ggaaaaaatt cccaaagaac gctaccgcaa
      601 attttttgat gataattgcg tctgggacaa ggtgccggaa acctggccag acattgcccg
      661 ctatgtggcc agagcctcta tggtggaaaa aagtaatcca cccgaaacgg tttccttgat
      721 tcgcttttcc agcaaaattc cgccgccgcc tatgcccgac gatttgccgc cgcctcaagg
      781 ccaggaccgg aagaaacaag aaaaaacaaa atgggaagcc aatccccctc actacgaaag
      841 taaactgctc atcaccctca aaattcatcc gcaagatctg gagggggtta agtaattgtc
      901 catcaaaact ctctttaagc tctgggacga gttctggttc accgagactt caccactggc
      961 ggtggcagtc tttcgtatct tgatgggcat catcatgata cttttcgtat tgctgctcac
     1021 ccaggacatg ttggtctggt tcggacagta tggactgata tccaatcaga cagagcgcaa
     1081 cttcgcccag gtagtcggtt tgaatgtcct caacctggcc agagacaacg atacttttct
     1141 gctggccttc ttcgtcgtat tttttgcggc cagcctgtgc atgaccatag gctacaaaac
     1201 cagactgtcg gtgttggtgg tctttctttg cctgaactct ctttatcatc gaaacgcctt
     1261 tttgtttaat tccggcgata cttatatgag ggtcatgtcg ttctggcttt tcttttcggc
     1321 cagcggcaat gccctatcaa ttgatcatct tctcaaactg aagagcaagc gcgcgcctga
     1381 aagcccggaa gaggcggaga agttcaacta caagtgtgtc agcaagtggc ccgtgcgcct
     1441 cttgcaatta caactggctc ttgtttactt ccataccttt gtggccaagt tctggggaga
     1501 cacctgggta aacggcacct ccgtttacta cagttcccgc atagaagacc tggtcagggt
     1561 aaaactgccc tacgtcttcg acaatctctg gacttgccag ttgcttacct ggggcacact
     1621 ggtcattgaa ctggctctct tcaccttaat ctgggtcaag gaatttcgtt actatgtaat
     1681 agccctggcc atagtcttcc atctcaccat cgatttgcac atgaacattc cgctcttcga
     1741 atggatcatg atgttctcct acgttctctt tatagataac caggatctgc aaaagggctt
     1801 gctcaaaatc aagcagaaat ttcccgcact cttcccagtg ctcaaggact gagataggtc
     1861 agcttctcat acttttgatc atctcttagc tgtctgagat tatcagcaat agccaccttg
     1921 aggctggcat tttcttttga agagcgggtg agaatcttac cggccttgag gaaacagtcg
     1981 gcagccacct cgtatcgttg accgcgctct gccagttccc gggtggagcc ggcccaaagg
     2041 taatacataa cgccttcatc attgagcaac ttggccttgg caatgccggc tgcgtcttta
     2101 tcaatggctt ccaggcgaga aacaatgcga ccggcctgac gaaagagcgg ttccgcttcc
     2161 tggaaaagcg tgcagtcaca cattacaagt gcagcctgcc gcttgaggtc aatgcgcagg
     2221 agcaaatggt ctacatcatc atactcaagg gccaccaact tctcggcact gagaagagta
     2281 tctctggcgg cagcaaaatt acggctctct tgataataga ttgccagttc cttcatggcg
     2341 cgggctttct caagagctga tttgccgctt tcttcgttga atcgcacctc ctccctggct
     2401 ctggtttcag ccaacttaag acgctcagat tgaagcgtct cattgtcata ggccaccaga
     2461 ccaccgtagg aaaccatgta aagcgtggta gatatagatg cgataaaggc caggctggcc
     2521 atccaggcga acaccctggc aaagagcttt ctctttgcat caattttgcc gactgtcgcc
     2581 aaaatcaacc accatagcta ataaaaagca gcgccacaaa tatattagcg cctcagattt
     2641 catgccccga aaaactgcgc cacaaacgta ggcaattgca gccttgaccg acccagatta
     2701 atgttaaaat caaaaagttt tttaaggcaa ccatcgtcac ccaagcaagg agtaatgaat
     2761 gtctaacgga catagcctga atctcaaaat gatgttagca gttctggctg catttttagt
     2821 gaccggctca gcttcggctc aaaatggtgc ccccacccct ggtgccgcca aggacagtca
     2881 caaaaaggaa gaagcaaaga caacggcttc ctccgagcaa aatcctgcct tgaagccggt
     2941 caatgagaaa atcttacccc tcctgaataa tggctatgaa ctcctcagca aagggcagta
     3001 cgataaggcc acccaggttc tggaaaaggc agtcaaggcc gacggcgaat caatttccgc
     3061 cagaagatac ctggctttcg cccaggtaag aaacggcgac aacctgaaag ccctttccaa
     3121 tctccaggcc ctttcccgct tggtcacccc cggcgccctc gattggtacg tcttcggcga
     3181 agcttatatg ggtgccggcg gtcttcaaca cgccaagtcc tgcttcaacc aggccctcac
     3241 cttcagtccc gactatgccg ccgcccgggg cggactggtg aagagccaga tcagactgaa
     3301 ggagtttgac gaagctttga gcacggttca ggacggtatc aataaaaccc gcgaccctca
     3361 aattcgtaaa tatttcagcg ccctctatca aaatgcggcg gcccagaagg aagccgccag
     3421 acgggccctc atcacctctc cggtgccgga atccagcacc accgccaccc aggaagagac
     3481 cggaccggtt ctgattaagc catccaatga cagctagttt gtagattatt tatttgcaga
     3541 ccgggcggac acgacccgcg cactgttgac aacacttttt ttgccgtgat aggtttgtaa
     3601 atagattaaa ctcgccctct tacgtcaact tttccaggtt gcgtaacagg tgttactaca
     3661 ttgctcgaat cgggaagaat cggtaaagat tctagttggg atagggaaag acatcactga
     3721 tggtgaatgg gaatgtttaa atacagacgc ggcactcgaa aaacgaaagg ggccatggc
//
LOCUS       DAOQNZ010000198         3768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11454_length_3768_cov_3.843523, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000198 DAOQNZ010000000
VERSION     DAOQNZ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11454_length_3768_cov_3.843523"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKW73_04840"
     CDS             complement(<1..397)
                     /locus_tag="PKW73_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNA72638.1"
                     /translation="MQSGNISNSEEKAAPAGLIKTLSEWAEMIKIEHTVFALPFALSG
                     LILSLPGKGFSSLPSVATVFWTVLAFASARCAAMTLNRLIDAGIDAKNPRTRDRAIPA
                     RRISRKTALLISVLSFTVMLLAAGNLPPIC"
     gene            477..986
                     /locus_tag="PKW73_04845"
     CDS             477..986
                     /locus_tag="PKW73_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72639.1"
                     /translation="MNFDGTGKTIDLQGVASFASLVDLLSQELGVPAGKMLGQLPNGP
                     DTFNQLLAEIDINSQGFLRKLTLTCQPGGFLSLRLATQWGSERNSSHTSGVTSDYHLT
                     ATVEQILPQARALARHIEDIFGVQDRMVTLGHSDKLAAASVSASQIEAFERQEGSSVV
                     TAHVPRGRE"
     gene            complement(914..2497)
                     /locus_tag="PKW73_04850"
     CDS             complement(914..2497)
                     /locus_tag="PKW73_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA72640.1"
                     /translation="MTNCNIALILEENASAFGTRTAIVDEEGRLLSFHGLNEGVKQQA
                     AALLAEGLKREQIVLLAIAPGIDFFVALLAILRVGARVLVIEPGSVKRVLSCLDAVRP
                     DFVLTVPWLVPLFACRYRTFTLSNRKSDQAPAAAFVDSKDGALITFTSGTTGSVKAIE
                     RSHGFLVQQAEVIDKSLALSTSPCTLTTLPVFTLAHLFYGVTTILVGKKSCRKALKLV
                     ERHQPKKILCAPAFLTGLLEGSAGQDLSCLDEVLVGGAPIFPSLVESVLARLPQSTVT
                     SVYGASEAEPICHFTVCSSHGAGYRRLHELAAEGQGLYLGKCEDRSVNLLMLSEADLA
                     RLLNQSRVTEEEISRLSAAEGHLLVAGKHVNQGYFNEQKGTNKLRVLQKDGTIKVYHA
                     TGDMCKMLAGELVLTGRAGYESKCKKGRYPLMVEAFAKTHDGIGEAAYVELDGQPLLA
                     VTLKGREANLEPELEAYCKKEFIKLVVMKKLPLDARHGSKVLYEKLKEELRLLGYSRP
                     RGTWAVTTEEPSCLSKASI"
     gene            complement(2500..3330)
                     /locus_tag="PKW73_04855"
     CDS             complement(2500..3330)
                     /locus_tag="PKW73_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72641.1"
                     /translation="MQSTTTDLMSLLSKSPDFLRSGRMYFADPGQACPEALLPLLEKT
                     ARECGATELVGPLAGNTFYRYRTVIEDSGALPFLCEPYNSNWSGLVDCLEQRGYRQLA
                     TYASKSVPLTKSPQLFAEKPAPVEFESFRVEAFEEELSAIYELTEKAFAANFLYSAIS
                     KEAFISLYEPLRATMDGELVLMARASATGKLVGFCLALRDTRAPRLILKTIARDPDPA
                     YRHLGLSMVCRMLELGRAKNFQEAIFALYKEGGFSSYMPDLFGGKTIRRYAVYARELS
                     "
     gene            complement(3366..>3768)
                     /locus_tag="PKW73_04860"
     CDS             complement(3366..>3768)
                     /locus_tag="PKW73_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72642.1"
                     /translation="AALLFLPGTTFLVDSNTLLLAYGMAFAIHGSIIASAHIRLVTQD
                     QRQLRKDRLVVLSINALKSWCLFFLPCVFMGGKNLSPWFTLLAFLYVLLFSLIFALRR
                     SPEESRTAARRWFRQGTLASLGSLGFLVCLA"
BASE COUNT          936 a         1008 c         1023 g          801 t
ORIGIN      
        1 gacaaatggg cggcaggttg ccggccgcca agagcatcac agtaaaggaa agaacactaa
       61 tcaatagtgc ggttttgcgg ctgatacgcc gggcaggtat ggctctatcc ctggttcgag
      121 gatttttggc atcgataccg gcgtcaatca gccggttcaa agtcatggct gcacaacggg
      181 cactggcaaa ggccagcacc gtccagaaaa cagtggcgac gctgggcagc gagctgaagc
      241 ctttgcccgg cagagagaga atcaagccgg agagggcaaa aggcagagca aaaaccgtat
      301 gctcgatctt gatcatctcg gcccactcgc tgagagtctt aatcagaccg gcaggtgccg
      361 ccttttcttc cgaattggat atatttccac tttgcatata cctaactgta cttgaagaat
      421 ttgccgacta ctggcatcat ttggaaggtt taagaatagc ctgagagaaa atgtatatga
      481 acttcgacgg aactggaaaa acaattgatt tgcaaggcgt cgcttccttt gcttccctgg
      541 tagacctgct ttcccaggaa ctgggcgtgc cggcgggcaa gatgctcgga caactgccga
      601 acggaccgga tacattcaac cagcttcttg ctgaaattga tatcaacagc cagggcttcc
      661 tgcgcaaatt gaccctcacc tgccagcccg gtggttttct cagcctgaga ctggctaccc
      721 agtggggcag tgagcgcaac agctcccata cctcgggcgt cacttccgac tatcatctga
      781 cagccaccgt ggagcaaatt ctgccccagg ccagagccct ggccagacat atcgaagata
      841 tattcggcgt gcaagatcgc atggtcaccc tcggtcattc cgataaactg gctgcggcaa
      901 gcgtttcagc ttctcagata gaagccttcg aaagacagga aggctcttcc gtcgtcactg
      961 cccatgtgcc ccgtggacgg gaatagccga gaaggcgcag ttcctccttg agtttttcat
     1021 agagcacctt ggagccatgt ctggcatcca ggggcagttt tttcatgacc acaagcttga
     1081 tgaattcctt tttacaatag gcttctagct ctggctccag gttagcctcc cgccctttca
     1141 gggtgacggc taacaaaggc tgcccatcaa gctccacata agcagcctcg ccgatgccat
     1201 catgagtttt ggcaaaggct tctaccataa gcggataacg ccctttcttg catttacttt
     1261 catagccggc tctgccggtc agaaccagct caccggcgag cattttgcac atatcgccgg
     1321 tggcatgata aaccttgatg gtaccgtcct tttgcagcac cctcagtttg tttgtgccct
     1381 tctgctcatt aaaatagccc tggttcacat gcttgccggc caccaataag tgcccctcgg
     1441 cggcactgag cctggagatt tcctcctcgg tgacccggga ctgattcagg agccttgcca
     1501 ggtcggcttc cgaaagcatg agcaagttta cggagcggtc ctcacacttg cccaggtaga
     1561 gtccctggcc ctcggcggcc aattcatgca ggcggcggta accggcacca tggctggaac
     1621 agacagtaaa atggcatatg ggctcggctt cgctggctcc gtatacggaa gtgacggtag
     1681 actggggcag gcgtgcgagc acggattcaa ccagagaggg aaagatgggt gcgccgccca
     1741 ccagaacttc atcgagacaa ctcaagtctt gtccggcact tccttccaga agcccggtta
     1801 gaaaagcggg ggcacagaga atcttcttag gctgatggcg ttcaacaagc ttgagagcct
     1861 tccggcaaga cttcttgccc accagaatag tggtaacgcc atagaacaaa tgggccaggg
     1921 taaagaccgg caaggtggtc aatgtgcaag gagaagtgga gagagcaagg cttttgtcta
     1981 tgacctcggc ctgttgaact agaaagccgt ggcttctttc aatggctttg acgctgccgg
     2041 tggtgccgga tgtaaaagta atgagggcgc cgtctttgct atcgacaaaa gcagcagccg
     2101 gtgcctggtc gctcttccta ttacttagag tgaaggtgcg atagcggcag gcaaataaag
     2161 gcacaagcca gggaaccgtc aaaacaaaat cgggacgcac cgcgtcaagg caggaaagta
     2221 cacgtttgac actgcccggc tcgatcacta gaaccctggc gccgacacgc agaatggcca
     2281 gtaacgctac aaagaagtcg ataccggggg caatggccag gagcacaatt tgctctctct
     2341 tgagaccctc ggcaagaagg gcggcggcct gctgctttac cccttcgttc aagccatgga
     2401 aagagaggag ccgtccctct tcatccacaa tggctgttcg cgtcccgaag gcagaggcgt
     2461 tttcttccag aatcagggca atattgcaat tggtcatcac taacttagct ccctggcata
     2521 aacggcatag cgccgtatgg tcttgccgcc gaacagatcc ggcatataag aggaaaagcc
     2581 gccctcttta tagagggcaa agatagcttc ttgaaagttt ttcgcccggc ccagttcgag
     2641 catgcggcag accatactta atcctaagtg acggtaagcg ggatcaggat ctctggcgat
     2701 agtcttgaga atcagtcttg gggcccgggt atctctgagc gccagacaga agcccaccag
     2761 ttttccggtt gccgaagctc tggccatcag caccagctca ccgtccatgg tggctctaag
     2821 gggctcatag agcgagatga aggcttcttt tgatatggca ctatacaaga aattggcggc
     2881 aaaggccttt tcggtcaact cataaatagc tgacagttcc tcttcgaaag cttccacccg
     2941 aaagctctca aattcgactg gcgccggctt ctctgcaaac aattgaggag atttagtgag
     3001 aggcacgctc ttggaagcat aggtggccag ttgtctatag cccctctgct caagacaatc
     3061 gactaacccg gaccagttcg aattatatgg ttcacacaaa aacggcaatg cgccgctgtc
     3121 ttcgatgact gtgcgatagc ggtagaaagt attgccggcc agaggaccca ccagttcggt
     3181 ggcaccacat tctctcgccg tcttctccag aagcggcaga agcgcctcgg gacaagcctg
     3241 cccgggatcg gcgaagtaca tacgaccgct ccttaaaaag tcggggctct ttgatagaag
     3301 gctcatcaaa tcggtggtag tgctctgcat ttttatcatc cctatttact agaacgtatg
     3361 cgcttctaag ccagacagac caggaaaccg agggagccca gggaggccag ggtaccctgc
     3421 ctgaaccagc gtctagcggc agtgcggctc tcctccggcg accggcgcaa ggcgaagatg
     3481 aggctgaaaa gtaaaacata gagaaacgcc agcaaggtaa accagggcga aaggtttttg
     3541 ccgcccataa agacacaggg cagaaagaag aggcaccagc tcttgagggc atttatggag
     3601 agtaccacca ggcgatcttt gcgcagttgc ctttgatcct gcgtgaccaa gcggatatga
     3661 gcactggcaa taatggaacc atgaatggca aaggccatgc cgtaggcaag caagagggtg
     3721 ttcgaatcaa ccaggaatgt ggtgcccggc aggaatagca gggcagcc
//
LOCUS       DAOQNZ010000199         3767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11460_length_3767_cov_3.252694, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000199 DAOQNZ010000000
VERSION     DAOQNZ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11460_length_3767_cov_3.252694"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..2101
                     /locus_tag="PKW73_04865"
     CDS             392..2101
                     /locus_tag="PKW73_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72643.1"
                     /translation="MLAVRTLLYYGMPVFQGSFFGYFEVLLVVLLSSNAVAWLLKMNP
                     GNIKYLLLCYLGLFIIPVSIHLMTANWGKTNSKRYADLPNIKQVAETETLPPTDPSHM
                     VLVNRDIAVFQGAKILQGEISSRYTIDKDSYTLQSIAGHRYWVAPLTLINTGDTLNWS
                     NPESPGYVVVDAENPEADPELKTGFHIKLFNDQSWALFVKRHAYQHGFSDMTLGKAFF
                     EVDDDWQPYWTFAYIKKPFGGMNGKVVDKIVMVNVAEAEPQVTSVNPKDKPDWLDRVI
                     DQELVFDYATDWGLYGRGHSKDYWPIFFNWNREGTLQPADVELNYTSDADNVWVVPMT
                     ATTGSHAVQGVLVFDTNRNDAKYYPGLNNFYIGESVRSTMFSVKENVMRYTVQSVQLY
                     SIYGELTWVGIYVAPQADGGSFAGIGLLHAHSQNPADVIFSTNKTTALSRYANQLANR
                     KQSGQIVSTAAKASKFITATIGRIAALPGSEVPTYLFVVPGDKNTYRITRETFSRIPL
                     IKEGDVVKFSYMDTNEEEEAVGSFSCIKCAGLDSSLPAEVQDSKIETIVPAPAAVEKQ
                     QSD"
     gene            2125..2463
                     /locus_tag="PKW73_04870"
     CDS             2125..2463
                     /locus_tag="PKW73_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72644.1"
                     /translation="MNNPNQKPKFPLTLEQERLCSWMLIWQGGVLLALILLANFLPVN
                     FGWIVVGMVPVMVDLYFTGGRTMAAAVGLSLLTALSVTVVLPLYTGLVFLGLGLALSI
                     HADKRSGKRS"
     gene            2483..2914
                     /locus_tag="PKW73_04875"
     CDS             2483..2914
                     /locus_tag="PKW73_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72645.1"
                     /translation="MMQTLVSEIASKIEKEERIMAGAVLGGLTSDQRLVKLSLYAYLL
                     ELARSLLLVPEERVREAGFELPLFGPICSPAIWGFVFAELQTLVQNPQSVECEIDLSG
                     PNVLEFRGLSYVELLGLANRLVDREVTLRTDKSDAFLEQAH"
     gene            3060..3698
                     /locus_tag="PKW73_04880"
     CDS             3060..3698
                     /locus_tag="PKW73_04880"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HNA72646.1"
                     /translation="MKKILFLLVLILLARVAVVFSPELSIFRLSSAIRHRDVQLFEKY
                     ADVHSVAQNLVHDQIAGPITGVFGSDGVGGWLTNGINSAVGPALVTGLEKEIRSAVFD
                     GAFNPDHPDDNSLVTVYRFQKELGFDQFAYSGMKYGDRQVLGEKAQLSLSYVRAADGY
                     ELVVKVELIKAPGEYIWKVSRILNPELVMEELRNHPAPASAATAVDHTYLPG"
BASE COUNT          878 a          934 c          967 g          988 t
ORIGIN      
        1 aaagaccaga aggaaagggc tgccgtagcc ggcagccctt tccttctgat atgcgaaatt
       61 cttttgccgc ccaatttgtt gcgcttttat gccgccgtca acactgagcc tcactgaata
      121 ccgtttcctt tcacggggaa gctgcgctgc attgcggcgt atgcggaagt tagcggtgtt
      181 ttcttccaat ccgaaacgag agaccaaaat gatattcgat tcacacttct ggatcttttc
      241 gctcttcgct tcctaagttg gtgctgcttt tacggcattt cttatctgga atccagagaa
      301 gctgggctgc cgaatttccg aaccgcatac ctggacccgc aagaacctgg ccagcatcga
      361 gtttgcagcc gttctttcct ttgtggtcac tttgctggcc gtaaggacct tgctttacta
      421 cggtatgcct gtctttcaag gcagtttctt tggatacttt gaagttctct tagtagtgct
      481 tctgtcgtcc aatgccgtgg cctggctgtt gaaaatgaac cctggcaaca tcaagtattt
      541 gcttctgtgc tatttgggtc tgtttattat cccagtgagc attcacctga tgactgctaa
      601 ttggggaaag accaattcca agagatatgc agacctgcct aacatcaaac aggttgctga
      661 aactgagacc ctgccgccga ccgatccctc tcatatggtt ctggtcaacc gtgacatcgc
      721 ggtttttcaa ggagccaaga ttctgcaagg tgagatttcc agccgctaca ccatcgataa
      781 agacagctac acgctgcaat ctattgccgg gcaccgttac tgggtagcac cgctgaccct
      841 tatcaacact ggtgatacgc tcaactggag caatcctgag agtccgggct atgtggtggt
      901 tgatgctgag aatcccgaag ccgatccaga gcttaaaacg ggttttcaca tcaagctttt
      961 caatgatcaa agctgggctc tgttcgtaaa gcgccatgcc tatcaacatg gtttcagtga
     1021 tatgacactt ggtaaagctt tcttcgaagt cgatgacgat tggcagccct attggacttt
     1081 cgcctatatc aagaagcctt ttggcggtat gaacggtaag gttgtcgaca aaatcgtcat
     1141 ggtaaatgtt gccgaagctg aacctcaggt caccagcgtc aatccaaagg acaagcccga
     1201 ttggttggac cgcgtcatag atcaggaact ggtctttgac tatgcaaccg actgggggct
     1261 ttatggtcgt ggtcattcga aggattactg gcccatcttt ttcaactgga atcgggaagg
     1321 tacgttgcag ccggccgatg ttgagttgaa ctacaccagc gatgcggaca atgtctgggt
     1381 tgtgcccatg acggccacta ccggtagtca tgccgtgcag ggcgtgttgg tcttcgatac
     1441 caatcgcaat gacgccaaat actatcccgg tttgaacaac ttctacatcg gagaaagcgt
     1501 cagaagtacg atgtttagcg ttaaggaaaa cgtcatgcgc tacacggtgc agagcgtgca
     1561 actttactct atttatggtg aactgacctg ggtcggtatc tatgtggcac cccaggctga
     1621 cggcggctcc ttcgccggta ttggactttt gcatgcccac agccagaatc cggccgatgt
     1681 cattttctca accaataaga caactgccct ttcgcgctat gccaaccagt tggcgaaccg
     1741 caagcaaagt ggacagatcg tcagcaccgc tgccaaggcc agcaaattta tcacagctac
     1801 gatcggacgc atcgccgccc ttcccggcag cgaagtaccg acttatctgt ttgttgttcc
     1861 tggcgacaaa aacacttatc ggatcactcg cgagaccttc agccgcattc cgctcatcaa
     1921 agagggcgat gtcgtcaagt tcagctacat ggatacaaac gaggaggaag aagccgtcgg
     1981 ctccttctcc tgtatcaaat gcgccggtct tgatagttct ttgcctgcgg aagtacagga
     2041 ctcgaagatt gaaacgattg ttccggcccc tgctgctgtg gaaaaacagc agtctgactg
     2101 acatttcaat agaacggaga atttatgaac aatcccaatc aaaaaccgaa atttcccctt
     2161 acactcgaac aggaacgcct ttgctcctgg atgctaatct ggcagggcgg agtcttgctg
     2221 gcactgattc ttctggctaa cttcctgcct gtcaattttg gctggatagt agtcggtatg
     2281 gtgccggtca tggtggatct ctactttacc ggtgggcgca ctatggccgc ggcggttgga
     2341 ctctcattgc tgacagcttt gagcgtcacc gttgttttgc ctctctatac ggggctggtc
     2401 ttcctgggtc ttggtctggc actgtctata catgccgata agagatccgg taagcgatct
     2461 tagctatcgg caagtggaga gtatgatgca aacacttgta agcgaaatcg caagcaaaat
     2521 tgagaaagaa gagcgtatca tggccggtgc ggtcttgggc ggtctcacct cggaccaacg
     2581 tctggtaaag ctgtctcttt atgcctatct gctggaattg gcccgtagcc tgcttttggt
     2641 tccagaggaa agagtgcggg aggcaggctt tgagttaccg ctcttcgggc caatctgttc
     2701 accggctatc tggggctttg tctttgcgga actgcaaaca ctggtgcaaa atccccagag
     2761 cgtagaatgc gaaatcgatc tgtccggacc caatgtgctt gagttccgtg gtctttcata
     2821 cgtggaacta ttgggcctgg ccaaccggct ggtggaccgt gaagtaaccc tgcgcaccga
     2881 taaaagcgac gcttttctgg agcaggctca ctgattgaat ccaaagtcgg tgccgttgta
     2941 tcgactgtgg aaagggtggc tatggtaggc cgccctgaga gtgcttcttt caaaccgtgc
     3001 tggagtgccg gtttgagagc gttgaaacag accatcaata aattttcggg agcaccatta
     3061 tgaaaaaaat cctcttcttg ttagtcctta ttcttcttgc aagagtggca gtagtctttt
     3121 cgccggagct ttccatattt cgcttgagta gcgctatcag acatcgcgat gtgcaattgt
     3181 ttgaaaagta tgccgatgtt cattctgttg ctcagaacct cgtacatgac cagattgccg
     3241 gccccataac cggtgtgttc ggaagtgacg gtgtcggcgg ctggctcact aacggcatta
     3301 atagcgcagt tggaccggct cttgtcacag gtctggagaa agaaattcgc tccgctgtct
     3361 ttgatggagc attcaacccg gaccatccgg acgacaattc actggttacg gtctatcgct
     3421 ttcagaaaga gttaggcttc gatcaatttg catacagcgg catgaaatat ggcgaccgtc
     3481 aggtgctggg ggagaaagcg cagctttctt tgagttatgt aagagctgcc gacggctatg
     3541 aactcgtcgt gaaggtggaa ttgatcaaag ctcctggaga atatatctgg aaagtttcaa
     3601 ggatattgaa tccggagttg gtcatggaag agttgcgtaa tcatccggcc ccggcctctg
     3661 cagccactgc ggtggatcac acttacctac ctgggtaatt cattttgtcg aaggtacaac
     3721 tcccggcaga cgcccttttt ctctgacacg acaaagcaca aacaaca
//
LOCUS       DAOQNZ010000200         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11519_length_3755_cov_3.103784, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000200 DAOQNZ010000000
VERSION     DAOQNZ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11519_length_3755_cov_3.103784"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKW73_04885"
     CDS             <1..509
                     /locus_tag="PKW73_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72647.1"
                     /translation="SPYPIKMPESLVNMAKTLTRCETDLVGPGFAPVPAGDPWGQEWL
                     IEHIRTYEKGGKCTLNMLPSTKELSVHTMTVAFLLANYPISISTFRRFTLNGDVFTCT
                     QGDIDYFDWYLVKDGFNGKNLADQESEKAYKALETLLATSPSFKLIGQKTLPDSSTIK
                     LYRRIKRP"
     gene            complement(525..3206)
                     /locus_tag="PKW73_04890"
     CDS             complement(525..3206)
                     /locus_tag="PKW73_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72648.1"
                     /translation="MGISLKGKVGPELGAEAQYAEPAPAQSRHLRAAAAVLFALVLAA
                     HLIHHFLFDHALVMFAFDGKHYLETTRLLTEAGQLLVSNPAAFNAAISAPHFISYMLW
                     DGPAIPGLPALVFLALGRLPTANDWMVYCLLLSSLAAGSAVLVMLICKDLYSAAASKS
                     RSSPALKSLTLPFLAGLAYGLYPSTLVATGVNRSETLATFLVMVFIYAGCRLLESRSA
                     LIVTGITGGLLTLTKPVLIPVLALLYAYFAVVPVSLSAVFTGSTGERLRIFSASFLRL
                     LLLGVVTIATISPWCLYSGVKAGKVAITAERGPSYNAGVGTDLEMDGWCTTPESNHVN
                     LMKVSDSPGSVLAAIWVKQPMELTALTLRRVARLWCDPWNDFREVVFGLSPQGQRYVH
                     VLMLFLGLGGGYLHLINLFRRKTASRSEESGSGSSFPALSSRQSLLLDLLVLFVLSHS
                     AFAVFQAMARYAFTAMAALYCLAFFLLLMAFRLKDGALRAYLLPSLLLSAGTLLALYS
                     IEPLSTAGRLQEEPVELSAGESLYRYLDITGTSARGAQALLLVDSDGACEGLKARVNG
                     VELSGSFRPFNQFSSSHYFLFDTMAEHGAQMRTAVQDFRQWRALPVNGALLKDGRNVL
                     QVTNGAGVPLTIYGDRPGAGAFCSSPQMSWRLPSPDYFGSDRMLVSVTSLESRIASLR
                     PAFGFKAQGKEVQPRLLLALSSSPRFGASPVSTQLRVPFQSVSSVTLPLDCKRFHWFS
                     RGEEDTLVVNKDVLKAVGVLTTQLPCRLTSDGNAPLTQVRLTLRGQARCLSGPGRLGV
                     DVAVKSPSRMQGLGCVPDFISIGKDWTDFSVSDMVPLAFMGGNIDGVSVGFAPCPRLE
                     TQYGPGRHCSNLRLRNLQVTIEGVASYDLGYGKIRYY"
     gene            complement(3221..>3755)
                     /locus_tag="PKW73_04895"
     CDS             complement(3221..>3755)
                     /locus_tag="PKW73_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72649.1"
                     /translation="VIEHVKNPEEFAESLHRACKGNWNIKLVISTGNIGFIIPRLMHL
                     FGQFNYGKKGILDLTHTRLFTFDSLRRLLEESGFVVLSEKGIPAPFPHAASNRKLADF
                     LLNLNDKFISLWKELFSYQIFVVVKPKPSLPSLLKDAVEHSRLKGLALDAVVPDRALS
                     GTTHSLERELSASASTV"
BASE COUNT         1053 a         1007 c         1029 g          666 t
ORIGIN      
        1 tcagccctta tccgatcaag atgccggaaa gtctggttaa catggcaaaa actctgactc
       61 gctgtgagac agacctggtg ggtcccggtt ttgcaccggt gccggccggc gacccctggg
      121 gacaagaatg gctaatcgag cacattcgca cctatgaaaa aggcggcaag tgtaccctca
      181 acatgctgcc cagtactaaa gaactgagcg tacacaccat gacggtagcc tttctgcttg
      241 ccaattatcc catcagcatc tccaccttcc gccgctttac tctcaacggt gatgtcttta
      301 cgtgcaccca gggtgacata gattactttg actggtacct ggtcaaagac ggcttcaacg
      361 gcaagaatct ggccgaccaa gaaagtgaga aagcctacaa agcattagaa acactgctag
      421 ctacaagccc atcttttaag ctgattggac agaagacctt accggacagc agcacaatca
      481 agctctaccg gcgcatcaaa cgtccctgaa aacgaataac aaacttagta gtaacgaatc
      541 ttgccataac cgaggtcgta agatgctaca ccttctatgg tcacctgcag gttgcgaagg
      601 cgcaagttgg agcagtgccg acccggacca tattgagtct ccaaccgcgg acaaggcgcg
      661 aagcccacag aaacgccgtc tatattgccg cccatgaaag ccaggggcac catgtcgctg
      721 acggaaaaat cagtccagtc tttgccgata gagataaaat caggcacaca gccaagcccc
      781 tgcatgcgag agggggattt gacggcgacg tcaacaccaa gccgacccgg accggaaaga
      841 caacgggcct gtcctcgcaa agtgaggcga acctgagtga ggggagcgtt cccgtcggag
      901 gtgagacggc aaggcagttg agtagtaaga actcccacgg ccttgaggac atccttattg
      961 accaccaggg tatcttcttc gccgcggcta aaccagtgga aacgcttgca gtcaagggga
     1021 agtgtaacac tgctgacgct ctggaagggc accctcagct gagtggaaac agggctcgct
     1081 ccgaagcgcg gactactgga aagagccagt aaaagacgag gttgcacttc ctttccctgc
     1141 gccttaaagc caaaggcagg gcgcaaggaa gcaatgcggg attccagaga ggtcacggaa
     1201 acaagcatcc gatcggagcc gaaataatca ggcgaaggca ggcgccagga catttgcgga
     1261 gaggagcaga atgcaccggc accgggtcgg tcaccgtata tggtgagggg tacgccggca
     1321 ccattggtta cttgcagaac gtttcgtcca tctttcaaga gggcgccatt gaccggtaag
     1381 gcacgccact gacgaaaatc ttgcacggcg gtccgcattt gggctccatg ttcggccatg
     1441 gtatcaaaaa ggaagtagtg ggaggaacta aactgattga atggtcggaa gctaccactt
     1501 agctcaacgc cattgactcg agccttcagc ccctcacagg ctccgtcact gtcaacaaga
     1561 aggagagcct gagcgccccg ggccgaggtc cccgttatgt ccagatagcg ataaagactt
     1621 tcaccggcgg aaagctccac cggctcttct tgcaatcgtc cggcggtgga gagaggttcg
     1681 atactgtaga gagccagaag tgtaccagca gacaggagca gggacggcag taagtaagcc
     1741 cgcagcgctc catccttgag cctgaaggcc attagcagaa ggaaaaaggc caggcaataa
     1801 agggccgcca tagccgtgaa tgcatagcga gccatggcct gaaaaacggc aaaagcgcta
     1861 tgactcaaaa caaacaagac cagtaaatca aggagcaaac tctgcctcga gctgagtgcc
     1921 ggaaaggatg acccggagcc gctttcttca gaccgagacg cggtctttcg tctgaacagg
     1981 tttatgagat gaaggtaccc gccgccaaga ccaagaaaga gcatgagcac gtgaacataa
     2041 cgctgccctt gaggggagag accaaaaacc acttcccgga agtcattcca tggatcgcac
     2101 cagagcctgg ccacccggcg cagagtcagc gcagtcaact ccatgggctg cttcacccaa
     2161 atggctgcca acacggagcc gggactatcg gagactttca tcaaattcac gtggttggac
     2221 tcaggcgtgg tgcaccagcc atccatctcc aaatccgttc ccacaccggc attgtaagag
     2281 ggtcctcttt ctgcggttat cgccactttc ccagccttca cccctgagta aagacaccag
     2341 ggagaaatcg tagcaattgt cacaacccct aaaaggagaa ggcgcagaaa ggaagccgaa
     2401 aaaattcgaa gtcgctctcc tgtggagccg gtaaaaacag cggaaagaga aaccggaacc
     2461 acggcaaagt aagcataaag caaggccaat accggaatca aaaccggctt agtcaaggtc
     2521 aacaaaccac cggtgatgcc ggtaacaata agggccgaac ggctttctaa aagacggcaa
     2581 ccggcataaa taaagaccat aaccaggaaa gtagccagcg tctccgagcg attcacaccg
     2641 gtagcaacga gagtagaagg atagagaccg taggcgagcc cggctaaaaa aggcaaggta
     2701 agagacttca gcgccggaga ggaccggctt tttgaggcag cggcgctgta aaggtctttg
     2761 caaatcagca tcaccagaac cgccgaacca gcggccaggc tggacagtaa caggcagtag
     2821 accatccagt cattggcagt cggcaaccgg cccagggcaa ggaaaactaa agccggcaga
     2881 cccgggatag ccggtccatc ccagagcata tagctgatga aatggggcgc cgatatggca
     2941 gcgttgaagg cggccgggtt ggaaacgagc agttgcccgg cttcggttag caagcgggtg
     3001 gtctccagat aatgcttacc gtcaaaggcg aacatgacca gtgcatggtc aaagagaaaa
     3061 tgatggataa ggtgtgcggc cagaaccagt gcgaacaaaa ccgcagcggc cgcccgtaga
     3121 tgccgagatt gagccggcgc aggctcggcg tactgcgctt cagcccccag ttccggaccg
     3181 accttgccct tcagcgaaat gcccaagtca tcacctgctg tcaaacagta gaagcgcttg
     3241 cactgagttc gcgctccaga gagtgggtcg taccagacaa tgctctatcc ggcacaaccg
     3301 catcaagggc cagccccttc aaacgactgt gttcaacagc atccttgagc agagaaggca
     3361 gcgacggctt tggttttacc accacaaaaa tctgatagga gaatagctct ttccagaggg
     3421 agataaactt gtcgttcaaa ttgagcaaga aatcagccaa cttgcggtta gaagctgcat
     3481 gggggaatgg tgccggtata cccttctccg aaagaacaac gaagccggac tcttccaaga
     3541 ggcgccgcaa cgagtcaaaa gtgaatagtc tggtgtgggt gagatccagg atgcccttct
     3601 tcccgtaatt gaactgaccg aagaggtgca tcaagcgggg aattataaag ccgatgttgc
     3661 cggtggaaat gaccagtttg atgttccaat tacctttgca agcccggtgc agactttcgg
     3721 caaactcctc gggattcttg acgtgctcga tcaca
//
LOCUS       DAOQNZ010000201         3743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11576_length_3743_cov_4.200380, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000201 DAOQNZ010000000
VERSION     DAOQNZ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11576_length_3743_cov_4.200380"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            478..2523
                     /locus_tag="PKW73_04900"
     CDS             478..2523
                     /locus_tag="PKW73_04900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA72650.1"
                     /translation="MNHATESKENSSNTTSPPADGVDLIKDALIKAGKNEEEISSVTT
                     VDAAQTAVDQGRGKPGVLQQALLQKQLSLDHFGCLAASDQLTSGKAAETLKLSLEAIQ
                     NHVAAGTILGEDGKVSAALIDDLAKAGYWGLPIPEEWGGSGASKYFCGKAMIEMGSKG
                     AEVVGGLLSIERLIGAAGPLIWKGTREQKQEFLTAMAAGHVRSGFGGTEPSAGCNITS
                     ILTHGVDDGDDILVYGEKLFISNAWYGHLIALLIRYEDKLRVLLVPLPMQDSDEFNIV
                     HYGIHALRQIHNKGLRFNGLRVPKKNLLPGDGLSIIFHDLDDGRFAVAATAIARMRRI
                     LASGLPWVYTRETFGEKLKERQYIRYLMALQAAYITSAEALVDWSASLIDEGYQGDVS
                     SMIVKTRVTDWLRQTATELGMFMHGGRFVLHNHTIGDNLADDMVSSVYEGPNPMLGKA
                     AIKAMVKPFGQEYLQPLMDNMKASGVNTQALRFGGFKQIMGTLGHLWSVKGGLFKNRG
                     KLTRDVFKLMGFLRMLSKKSKPDVGTASVLKDLPKSFTGHMNFANKAWRVWRKNFILQ
                     VLKYQERLVDEDLIMLEEIYEPLTQITTMLVSIKAAQAAAKRGDAAAVAALNLTCLEM
                     RVKLGGEKRTSVAYRKSVKEVSEHVLTGTFMPVQGVPTGAILQPYSVPAATAKTGKK"
     gene            2735..>3743
                     /locus_tag="PKW73_04905"
     CDS             2735..>3743
                     /locus_tag="PKW73_04905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNA72651.1"
                     /translation="MNTSDTSSNAGNTGSARKVLIGAYVRTPIGKVGGKLSRLEASDL
                     AAHALRSAVKRAGIKEAGLIDLVVAGHAVQSYFEPNTARIAMQKAGLPDSISAYTIQQ
                     QCASGMNAAHAVFAAIKNGEAELGLAVGVESMSLPPLIVSGHARYKGMNRWLTTKAPK
                     FLRKFFKTYGPLPFFGLADSGLGPLKQAKDPSVLNMIKTAQIVADLCGITREQADYFA
                     NRSQSLALTAIKNGRLAREIEPLVIPGVGVVATDEFPRKTDMTALSGLPEQARSRVVT
                     AGNASGMGDGAGALVLCTEEMAARLGIVPLAELVDVTFSARHAVSMGLGPVGAVNDLL
                     AR"
BASE COUNT          869 a         1166 c          980 g          728 t
ORIGIN      
        1 ccacggacgg ttgccgacct tccggcgtga cagtttcagc ccggtcaatt ccgtatcaag
       61 ggaagtagca cgaaaacacc gcgctgtagc attttgactg ttctagatag tgaattcatg
      121 ggcctcgatc tgctaggaaa ggttgtccct ttttcctttc ctttcttgag caagtcaaac
      181 tctttagttt cttccatcac ccaacacatg agcccaaacc ctgcccgcta gacgattagc
      241 actcgggtga aagaagctca cttttccctc agagatggaa gttccccggt tctgccacca
      301 ccgcctcaag aaacgaatat ccaaccggcc caaacagtcg ttcccctcgg ggctccgcct
      361 gaacggccga actcggctcc actagcgatg ccacctgcca ccgctccacc agcggtgcat
      421 tcaccggcac ggctcctgct gtgctctctc aaaccaatca acctgacagg aatttctatg
      481 aaccacgcaa ctgagtcaaa agaaaatagt tctaatacca ccagtccacc ggctgatgga
      541 gtcgatctaa ttaaggacgc tctcatcaag gctggtaaaa acgaggaaga aattagcagc
      601 gttactaccg tcgacgccgc acaaaccgcc gtcgatcaag gacgcggcaa accaggcgtt
      661 ttgcagcaag cccttttgca aaagcagctt agcctcgacc acttcggctg cctggccgct
      721 tcagaccaac tcaccagcgg caaagcggct gaaactctca aattgagcct ggaagccata
      781 cagaatcacg tagcggccgg cactatcctg ggcgaagacg gcaaagtcag cgccgctctc
      841 atcgacgacc tggcaaaagc cggctactgg ggtctgccca ttcctgaaga atggggtggt
      901 tccggcgctt ccaaatactt ctgcggcaaa gccatgattg agatgggctc caaaggcgcc
      961 gaagtagtag gcggtctctt gtctatcgaa cgccttattg gcgccgccgg accactgatc
     1021 tggaaaggca ccagggaaca gaaacaagag ttcctcaccg caatggccgc agggcatgtg
     1081 cgctccggct tcggcggcac cgaaccaagc gcaggctgca acatcacttc gattctcacc
     1141 cacggtgtag acgacggtga tgatattctc gtctacggcg agaagctgtt catctccaac
     1201 gcctggtacg gtcatctgat cgcactcttg attcgctacg aagacaagct ccgggtcttg
     1261 ctggtgcctc tgcccatgca ggacagcgac gagttcaaca tcgtgcacta cggcatccac
     1321 gccctcagac agattcacaa caaaggcttg cgcttcaacg gtctgcgcgt gcccaagaag
     1381 aatctcctgc ccggcgacgg actgtccatc atctttcacg acctcgacga tggtcgtttc
     1441 gctgtagctg ccactgccat tgcccgtatg cgccgcattc tggccagcgg tctgccctgg
     1501 gtctataccc gcgaaacctt cggcgaaaag ctgaaagaaa ggcagtacat ccgctacttg
     1561 atggccttgc aagccgccta catcaccagc gcggaagccc tggttgactg gtcggccagc
     1621 ctcatcgacg aaggttacca gggcgacgta tcttccatga tcgtcaaaac ccgggtcacc
     1681 gactggctga gacagaccgc caccgaactg ggcatgttca tgcacggcgg tcgcttcgtc
     1741 ttgcacaatc acaccatcgg cgacaacctt gccgacgaca tggtctccag cgtctatgag
     1801 ggtcctaacc cgatgctggg taaggccgcc atcaaagcca tggtcaaacc cttcgggcaa
     1861 gagtatttgc agcccctcat ggacaacatg aaggccagcg gcgtcaacac ccaggcgctc
     1921 agattcggcg gcttcaagca aatcatgggc acccttggtc acctgtggtc ggtcaaaggc
     1981 gggctcttca aaaaccgcgg caagctcacc agagacgtat tcaagctaat gggcttcctg
     2041 cgcatgctgt ctaagaagag caagcccgat gtcggcaccg caagcgtgct gaaagacctg
     2101 ccgaagagct ttacagggca catgaacttc gccaacaaag cctggcgggt ctggcgcaaa
     2161 aacttcatcc tgcaagtgct caagtatcaa gagcggcttg tggacgaaga cttgatcatg
     2221 ctggaagaaa tctatgagcc cctcactcag attactacca tgctggtctc catcaaagcc
     2281 gcccaggccg ctgccaaaag aggcgacgcc gctgctgttg ccgcgctcaa cctgacctgc
     2341 ctggaaatgc gggtgaaatt gggcggtgaa aagcgcacca gcgtcgccta cagaaagtcc
     2401 gtcaaggaag tttccgagca tgtcctcacc ggtactttca tgccggtgca aggcgtaccg
     2461 acgggtgcca tcctgcaacc ttacagtgtg ccggcggcaa ccgccaagac cggcaagaaa
     2521 taagccctct gccgctctcg ctcttggcgc cggcccacgg tcgacgccag ttgcagagag
     2581 cacacaccgc acctgcagac tgaagggccg gcaatggcat agccatgctc ctgccgcagt
     2641 ctgcagcaac cgaagagagc tcaatctctc ctgggatgtc ccgggaagcg agcgagctct
     2701 cttttctaac cgacaaaacc tgacgaggtt ccaaatgaat acttcagaca cttcatcaaa
     2761 tgccggcaat accggctcag cacgcaaagt cctaatcggc gcctatgtgc gcacccccat
     2821 cggtaaagtc ggcggcaaac tttcccgctt ggaagcatca gatctggcag cccatgccct
     2881 gagaagcgcc gtcaaacgcg ccggcatcaa agaagccgga ttaattgacc tggtggtagc
     2941 cggccacgcc gtgcaaagct atttcgagcc caatacggct cgtatcgcca tgcagaaagc
     3001 cggtctgccg gacagcatca gcgcttacac cattcaacag caatgcgcat cgggcatgaa
     3061 cgccgcccat gccgtctttg ccgccatcaa aaacggcgaa gcggaactgg gtctggctgt
     3121 gggcgtcgag tccatgtctt tgccgcccct gattgtctcc ggtcatgccc gctataaagg
     3181 catgaaccgc tggttgacca cgaaagcacc gaagttcttg cgcaagttct tcaagaccta
     3241 cggtccactg ccgttcttcg gtctggccga cagtggtctg ggaccgctca aacaagccaa
     3301 agatccctcc gttttgaaca tgatcaaaac ggcccagatc gtagccgacc tgtgcggcat
     3361 caccagagag caagcggatt atttcgcaaa ccgctcacag tctctggctc tcactgccat
     3421 caagaacggt cgcctggccc gggaaatcga acccctggtc attcccggcg tgggcgtggt
     3481 ggccaccgac gaattccctc gcaaaaccga catgacggcc ctgtcaggct tgcctgagca
     3541 agccagaagc cgcgtggtca ccgccggcaa cgccagcggt atgggcgacg gtgccggtgc
     3601 gctggtgctc tgcacggaag aaatggcggc aagactgggt atcgtgcctc tggctgagct
     3661 ggtggacgtt accttctcgg ctcgccacgc cgtttccatg ggtcttggtc ctgtcggcgc
     3721 cgtcaatgac ttgctggcaa gac
//
LOCUS       DAOQNZ010000202         3742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11585_length_3742_cov_3.218606, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000202 DAOQNZ010000000
VERSION     DAOQNZ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11585_length_3742_cov_3.218606"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PKW73_04910"
     CDS             complement(<1..1109)
                     /locus_tag="PKW73_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72652.1"
                     /translation="MPISRGLAAFLCLTALLLLGFGNTLTPYFHCDDFLHTAYLYRVF
                     YSDFWLLGTNFFSSWLQDRSFYNFFRPLTEISLALDYGLYEGQARGYHLTNLLLHLVN
                     CVLVFKLTATLAARYELAPGKTCFLNAFFAAALFATLGTHSEAVAWILSRSDLVSTAF
                     YLASLLCFLKSESADSHKQLFQLLAGAAFGLALLTKEMAVSLPFLITLLSFTSPAQEQ
                     KQTVVWKTILPYFLLLGFYLVWRSLSIGTIYGSYAGSLGEIMRASMWERWFLSDELLR
                     FFHPFNIDLIDENSPLRGALRLVLAGCGLLVLLIQLSCRNSRNLRQETQLSNRGRLIF
                     TLLAFTLLAALPSLQILGVTANMTGGRIAYLPSVPA"
     gene            complement(1096..2040)
                     /locus_tag="PKW73_04915"
     CDS             complement(1096..2040)
                     /locus_tag="PKW73_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72653.1"
                     /translation="MSIQDTEGKASPSQSTSERRHGTVSNTIMLVLAAGLVALAMSYP
                     KHTARGGPYGQPDPTPPPPPVITPVIPPVERPPIVVPTDVTNINLVNPIVVPNLQVSP
                     ALSGVQLLPDSQFPPLDKVQQNEDWITILSVEGSVFTKPNGYTVDLKSGEIIVSVKSP
                     SKIAFVITPFGTLAMSANSDVLVSFKDGVLRVMNFDGDGMALKAQLDKGPFAGPADPT
                     VTLACGYELVAANEKITRAELRPKDGITRRFAKVLENGHLAVSEFSVESALKNCAMLV
                     DLSQKVSGVKERRMLSDMSKMAAVLNYKHGTEGFSADK"
     gene            2305..>3742
                     /locus_tag="PKW73_04920"
     CDS             2305..>3742
                     /locus_tag="PKW73_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNA72654.1"
                     /translation="MQDFEKLGVFYLGRRYDLKTESASQDLVLYDSADLVTHAVCIGM
                     TGSGKTGLCLTLLEEAAMDGIPVIAIDPKGDIGNLLLTFPDLDAQSFAPWIDEDEARR
                     LGISVQELANRESSRWRQGLQETGQDGERIARLRENCRFAIYTPGGTAGRSISITHFL
                     SKPDEETLEDGELFNERVASAATAVLSLVGIEGDILTSREHILLSSIFASVWQSGQDI
                     ELASLIALIQNPPLTRVGAIDLESFFPQKERFALSLKLNNLFASPGFAAWLNGEPLDV
                     DRLLYSEEGKPRISIFSISHLSDEQRMFFVTLMANQMLGWMRRKSGTKSLRAIFYMDE
                     MLGYFPPVANPPSKTPILTLLKQARAFGLGLVLATQNPVDLDYKGLANTGTWFIGRLQ
                     TEQDKMRVIDGLEAATAGSGQVFSRADLLANLSRLGKRVFLMNNIHEDQPVIFQTRMT
                     MSYLKGPLTRQEIKSLSADKSDRAQGVAA"
BASE COUNT          845 a          942 c         1099 g          856 t
ORIGIN      
        1 gccggtactg aaggcaaata ggcgatgcga ccgccggtca tattggccgt gacgccgagt
       61 atctgcaggc tgggcagcgc tgctagaaga gtaaaagcta aaagagtaaa aatcagtctg
      121 ccacggttac tgagctgcgt ttcctggcgt aagtttcgtg agtttctgca cgaaagttgg
      181 atcagcaaga ctaaaagccc gcaaccggcc aggacgaggc gcagggcccc gcgcagaggg
      241 ctgttctcat cgattaagtc gatattgaag gggtggaaga atcgcaaaag ctcatcgctc
      301 aggaaccagc gttcccacat ggaagcgcgc atgatttctc ccagggagcc ggcgtagcta
      361 ccatatatgg tgccgatact gaggctgcgc cagaccaggt aaaagccaag cagaaggaaa
      421 tagggcagga ttgttttcca gaccacagtc tgtttttgct cttgcgccgg gctagtgaag
      481 ctgagcagtg tgattagaaa gggcagggat acggccattt ccttggtcag gagagccaga
      541 ccgaaggctg ccccggccag aagctgaaat agctgcttgt gggaatctgc gctttcgctc
      601 ttcaaaaagc aaagcaggct tgccaggtag aaggcggtag agacgagatc cgagcgcgat
      661 aaaatccagg ccaccgcttc gctatgggta ccgagagtgg cgaagagagc agcggcgaag
      721 aaggcgttga ggaagcaagt cttgcctggt gccagttcat aacgtgcagc cagtgtggcg
      781 gttagcttga agaccaggac gcaattgacc aggtgcaaga gcagattggt cagatggtag
      841 ccccgggcct gcccttcata gagaccataa tcgagagcca gactgatttc ggtcagaggg
      901 cgaaaaaaat tgtagaagga gcgatcttgc aaccaggagc tgaagaaatt tgttcccaga
      961 agccagaaat ccgagtaaaa gacgcggtag agataagccg tatgaagaaa atcatcgcag
     1021 tgaaagtaag gcgtcagtgt gttgccgaag cccagaagca atagggcggt taggcaaaga
     1081 aaggcggcta ggcctctact tatcggcact gaagccttcg gtaccatgtt tgtaattgag
     1141 caccgccgcc atcttggaca tgtcggagag catgcgccgc tctttcacgc cgctcacttt
     1201 ctggctcaaa tccacaagca tggcgcagtt tttcaaggcg ctttctactg aaaactcgga
     1261 gactgccaga tgcccgttct caagtacttt ggcgaaccgc ctggtgatgc cgtctttagg
     1321 acgcagttcg gctctggtga ttttttcgtt ggcagccacc aattcgtagc cgcaggccag
     1381 cgtcaccgtg ggatcggcgg gaccggcgaa aggacctttg tcgagctgag ctttgagggc
     1441 catgccgtct ccgtcaaagt tcatcactct cagcacgccg tctttgaagc tcaccagcac
     1501 gtcggagttg gcgctcatag ccagggttcc gaagggcgtg atgacgaagg cgattttgga
     1561 aggactctta accgaaacta taatttcgcc cgatttcagg tcgacggtgt agccgttcgg
     1621 tttggtgaag accgagcctt ctaccgataa aatggtgatc cagtcttcat tttgctgcac
     1681 tttatcgagg ggcggaaact gactgtcggg caatagttgc acacccgaca gcgccggact
     1741 cacttgaaga ttgggcacca ctatggggtt gaccagattg atgttggtga cgtcggtggg
     1801 caccacaatc ggcggccgct ctactggtgg tatcaccggc gtgattaccg gcggaggggg
     1861 gggggtgggg tcgggctgtc catagggacc gccccgggcc gtgtgcttgg gataggacat
     1921 cgccagggcc accaagcccg cagccaggac gagcattatg gtattggata cagtgccgtg
     1981 cctgcgctca gaggtgctct gggacggact tgcttttcct tcggtgtcct gaattgacat
     2041 ggcttttgtc ctgaatttgg ctggttataa tctcgctgga gatatatcta gttttctacc
     2101 tgtttggaaa ttatagccca agacggcggc atctttaaaa gctggtttac aagaagcggc
     2161 ttgccggtgg agctagcggt cgcctggagc ggtagcatca ttaagcctta ccggggtccg
     2221 tccctatttt gggggcgggg ctgctatttt aatagtcggc cgggggcttt ccagcaggtt
     2281 aaagggaatt caaacagggg ttttatgcaa gatttcgaga aactgggtgt gttctatctt
     2341 ggtcggcgtt acgatttgaa gactgagagt gcctcgcaag atctcgtcct ttacgatagc
     2401 gccgacctgg ttacccacgc cgtctgtatc ggcatgaccg gtagcggcaa gaccggtctt
     2461 tgcctcactc ttttggaaga ggcggccatg gacgggatac ccgtcatagc catcgatccc
     2521 aagggggata taggcaattt gctcctcacc tttccggatc tggacgccca atcttttgct
     2581 ccctggatag atgaagatga ggcccgccgt cttggtattt cggtgcagga attggccaat
     2641 cgagagtctt ctcgctggcg tcaaggtctt caggaaaccg ggcaggacgg agagcgaatt
     2701 gcccggctga gggagaattg ccggttcgcc atttacactc cgggcggtac ggccggtcgc
     2761 tccatctcca tcacccactt cctctcaaag cctgatgagg aaaccctgga agacggggaa
     2821 ctctttaatg agcgggtggc cagtgccgct accgccgtac tttccctggt cggtatagag
     2881 ggggatattc tcaccagtcg tgagcatatt ttgctttcct ctatttttgc ttcagtctgg
     2941 cagagcggcc aggacataga actggcttct cttattgccc tgattcaaaa tcctcctctg
     3001 acaagggtcg gggccatcga tctggagtcc ttctttcccc agaaagagcg ctttgcactc
     3061 tcgctcaagc tgaacaatct cttcgcctca ccgggctttg ccgcctggtt gaacggcgag
     3121 ccgctggatg tagaccgctt gctctatagc gaagagggta agccccgtat ttcaattttc
     3181 tccatcagcc acttgagtga tgaacagcgc atgttctttg tgacgctcat ggccaaccag
     3241 atgttgggat ggatgcgtcg caagtctggg accaagagcc tgcgggcaat tttctacatg
     3301 gacgagatgc tgggttattt ccccccggtg gctaatccgc cttccaagac acctattttg
     3361 accttgctta aacaggccag ggcttttgga ctgggactgg tgctggccac gcagaaccct
     3421 gtagacctgg actacaaggg gttggccaac acgggcacct ggtttatcgg gcgattgcaa
     3481 accgagcaag acaagatgcg agtcatagac ggattagaag cagccacggc cggttccggc
     3541 caggtcttca gccgtgccga tttgcttgct aacttatctc ggcttggcaa gcgggtcttc
     3601 ctcatgaaca atatacatga ggaccagccg gtgatttttc aaacccgcat gaccatgtct
     3661 tatttaaaag gacctctgac ccgccaggaa ataaagagtc tatctgccga caagtcagac
     3721 cgggcgcagg gggtggctgc tg
//
LOCUS       DAOQNZ010000203         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11608_length_3738_cov_3.750204, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000203 DAOQNZ010000000
VERSION     DAOQNZ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11608_length_3738_cov_3.750204"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PKW73_04925"
     CDS             complement(<1..964)
                     /locus_tag="PKW73_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72655.1"
                     /translation="MTDSRANKRKPSRLSSIIFGLILILGLSQPASVLAQNNEAGEQD
                     PGLDQFQTLSPSSREMAIDLGIQDDLTALFSLRARRNKRLAAQGQGTTGNDTTKVGSL
                     AADDPDALQIVLLRQEINETILSAVLELRVVISKIDNEITAYNAVRSYLEDRRDRSIR
                     LNNISNFIVSGALNMISNGLEIPPGETPETVGAMIGVLGGATSTGISAWSLKIAGGEK
                     RTATIAPNMLAKVFGLPTGAEHDYPRQIWRFFTNIPPGSTAKESRKERLMRRWLELKR
                     IDGLNTAEQKKRVALLCSTTPVVKGVDIDVLEDREAMLSDVKATL"
     gene            complement(918..1898)
                     /gene="cdaA"
                     /locus_tag="PKW73_04930"
     CDS             complement(918..1898)
                     /gene="cdaA"
                     /locus_tag="PKW73_04930"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNA72656.1"
                     /translation="MSNFADFLNQISWLTLAKVMVQLFVICYGVIWLWRRIAGTHAER
                     LVKGMLVLIFIAVLSWALQLTIITSMLQHVIPVAAMALVIVFQPEIRRGLGYLGRVQT
                     FKVDLSLLHTNQASISNHINEIIKAVRELSRNKIGALIVIEPPEGERDYVSPGTPVNA
                     DISATLLLTIFFPKSPLHDGAVVIRKAKIVAAGVILPMTDNQKLSYKYGTRHRAAIGL
                     SEIYDGLCIVVSEETGAISAASRGMLARYNSADDLIEPLGYLYNTGDKGGSGPLNSFL
                     SLFGRGGKNEVQELSLGTRDEPRVSNAGNSEPEIKHDRLTRKQAEAEQTL"
     gene            complement(1922..3259)
                     /gene="lysA"
                     /locus_tag="PKW73_04935"
     CDS             complement(1922..3259)
                     /gene="lysA"
                     /locus_tag="PKW73_04935"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNA72657.1"
                     /translation="MKSSNSPNQNIMPVSARVSKGNVLTLGGVSTEVLARDFGTPLWI
                     MCQDTIEAAAREIAAGLQHYPAGALPCYAGKAFLCTAMVRLVDKLGFGLDVVSKGEIE
                     TALAANFKGSIYLHGNNKTDEELALAVKHNLKVVADNQQDLERLVQLHSKTERTTPTE
                     VLLRIIPGIDLDTHDHIKTGHDTSKFGIPLSQLNDTIAYCLSEKSIKLMGLHAHIGSQ
                     AMDLEPFQQVIEVFADIYEEVSKRFSYVLPHLDIGGGLGIAYVASDKPIALEEWTRTI
                     SNKMVEAFQEKRGLPLPSLSIEPGRCLVGTAGVTLYRVGRMKQLEQINYLSVDGGMAD
                     NPRPITYQAAYTAMVANRMEPREEHLKTWTIVGRYCESGDIIVEEAQLDAAPGDIIAI
                     FATGAYNYSMASNYNRTARPACVLVKDGHSELMIAPETEEDLLRLDRIPSWLK"
     gene            complement(3295..>3738)
                     /locus_tag="PKW73_04940"
     CDS             complement(3295..>3738)
                     /locus_tag="PKW73_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72658.1"
                     /translation="PGTIILNEGIDNAALIYIFKGKIHGVFSFEEGWLENSRESIEKI
                     YRKLAHPDVQASKLLACNIWELKQCTFSVTGIEETPLSASQHTSLSLNYDELDHIAHD
                     KKDGGLSSSFKHKEELPKFVAGGSSQRVSQSYWKNKHENLSPDRC"
BASE COUNT          737 a          941 c         1069 g          991 t
ORIGIN      
        1 agagtgtggc tttgacgtcg gatagcattg cttccctgtc ttccagcaca tcgatgtcca
       61 cacccttcac cacaggcgtt gtggagcaga gcagggccac cctcttcttc tgctccgccg
      121 tattcagtcc gtcgatgcgc ttcagttcca gccagcgtct catcaagcgc tctttgcgcg
      181 attctttagc tgtcgagccg ggtggaatat tggtaaagaa gcgccagatt tgcctgggat
      241 agtcatgttc ggccccggtt ggcaaaccga atactttggc cagcatattg ggggcaatag
      301 tggccgtgcg cttctcgccg ccggcaattt ttagagacca ggcggaaatg ccggtggaag
      361 tggctcctcc caggaccccg atcatggcgc cgactgtttc cggagtttct cccgggggga
      421 tttccagccc gtttgaaatc atattgaggg cgccgctcac gataaagttg gaaatgttgt
      481 tcagtctgat ggagcggtct cgcctgtctt ccaggtagga ccggacggca ttgtaggccg
      541 ttatttcatt gtcgattttg ctgattacca ccctcagctc gagcacggcc gaaagtatgg
      601 tttcgttgat ttcctggcgg agcaaaacaa tttgcagggc atccggatca tcggccgcca
      661 gggatccgac ttttgtggtg tcattaccgg tggtgccctg accttgggct gccagtcgct
      721 tgtttctcct tgcccgcaag gagaaaaggg ccgtcaggtc gtcttgaatg cccagatcga
      781 tagccatttc tctggaactg ggagagagag tctggaactg gtcgagtccc gggtcttgct
      841 cgccggcttc gttgttttgc gccagcaccg atgccggctg gcttaagccc agaatcagga
      901 tgaggccgaa gataatgcta gagagtctgc tcggcttccg cttgtttgcg cgtgagtctg
      961 tcatgtttga tttcaggctc ggaatttccg gcattgctta ctcttggctc gtctctggtc
     1021 cctaatgata gctcctgtac ctcatttttt cctcccctgc cgaagaggga gaggaaggaa
     1081 ttaaggggac cggacccgcc tttgtcgccg gtattgtaga gataaccgag gggctcgatc
     1141 aggtcgtcgg ccgaattgta ccgggccagc atgccccggc tggcagcgga aatcgctcct
     1201 gtttcctcgg atactacgat gcagagtccg tcgtatatct ctgaaagacc gatggccgcc
     1261 cggtgcctgg tgccgtattt gtagctcagt ttttgattgt cggtcatggg cagaattact
     1321 ccggccgcca caatcttggc tttgcgaatg acaacagctc cgtcatggag aggggatttg
     1381 gggaagaaga ttgtcagtag taaggtggcg gaaatgtcgg cgttgaccgg ggttcccggg
     1441 ctgacgtagt cccgttcgcc ttcgggcggt tcgataacaa taagggcgcc gattttattt
     1501 cttgaaagct ctcttacggc tttgattatt tcgtttatat ggttggaaat gctggcctga
     1561 ttggtatgca gcaaagatag gtcgaccttg aaagtctgca ctcgacccag ataaccaagt
     1621 ccgcgtctga tctcgggttg aaagacgatg accagggcca tggctgccac cgggatgacg
     1681 tgctgcagca tggaggtgat tatggtcagt tgcagggccc aggagaggac ggcaatgaag
     1741 atgagtacca gcatgccctt cacgaggcgc tccgcatggg tgccggcgat acgtcgccag
     1801 agccagatga cgccatagca gatgacgaag agctggacca tgactttagc cagggttagc
     1861 caacttattt ggtttaagaa gtcagcgaag ttactcatgg ccggtatttc cagttgctat
     1921 ctcattttag ccagctcggg atgcggtcca gcctcaataa gtcttcttcg gtctccgggg
     1981 cgatcattag ttcagagtgt ccgtctttta caaggacgca agccggtctg gccgtgcgat
     2041 tgtaattgga ggccatgctg tagttgtagg cgccggtagc aaatatcgcg atgatatccc
     2101 ccggtgccgc gtccagttgg gcttcctcta cgattatatc ccctgactca cagtacctgc
     2161 cgactatggt ccaggttttc agatgctctt ccctaggttc catgcggttt gccaccatgg
     2221 ccgtataggc tgcctgataa gttatggggc gtggattatc ggccattcct ccatccacac
     2281 tcaggtaatt aatctgttcc aattgcttca ttcgtccgac cctgtagagg gtaactccgg
     2341 ccgtgcccac caggcaacgc cccggctcaa tggaaagaga aggcaggggg agaccgcgtt
     2401 tttcctgaaa ggcttccacc attttattgg agatggtacg ggtccactct tccagggcta
     2461 tgggtttgtc gctcgccaca taggcaatgc ccagtccgcc gccgatatcc agatggggca
     2521 ggacatagct gaaacgcttc gaaacttcct cgtatatgtc ggcaaagact tctattacct
     2581 gctggaaggg ttccaggtcc atggcctgac tgccgatgtg ggcgtgcagt cccattagtt
     2641 tgatggattt ttcgctcagg caataagcga tggtgtcgtt caactgcgag agcggaatac
     2701 cgaacttact ggtgtcgtga ccggtcttga tgtgatcatg ggtatcgagg tcgatgcccg
     2761 gtatgatgcg cagcaagacc tcggttggag tagttctttc cgttttgctg tgcaactgca
     2821 ccagtctttc cagatcttgc tggttgtctg ccactacctt caaattgtgt ttgacggcca
     2881 gagctaattc ttcgtcggtt ttgttattgc cgtgcaagta gatgctgccc ttgaaatttg
     2941 ctgccagggc cgtttctatc tcgccctttg agaccacatc gaggccgaag ccaagtttgt
     3001 ccacaagcct gaccatggct gtacagagaa aagctttacc ggcgtagcag ggcagtgctc
     3061 ccgccgggta gtgctgaagt ccggccgcta tctctcgggc ggcagcttca attgtgtcct
     3121 ggcacatgat ccagaggggg gtgccgaagt cccgggccag gacctcggtg ctgacgccgc
     3181 ccagggtgag cacgtttccc ttggacaccc tggccgatac gggcatgatg ttttgattgg
     3241 ggctgtttga agatttcaat tttctttgag tttccagaga tggcaggctc tagcctagca
     3301 tctatcaggg ctcaggttct cgtgtttgtt cttccagtag ctctggctca ccctttgtga
     3361 tgagccaccg gcaacgaatt tgggcagttc ttctttgtgc ttaaaggagc ttgatagacc
     3421 gccgtctttc ttgtcatggg caatgtgatc cagttcgtcg tagttgaggc tgagggaggt
     3481 gtgttggctt gccgaaagtg gtgtttcttc aatgccggtg actgagaaag tacattgctt
     3541 caattcccag atattgcagg ccagaagttt ggaggcttgc acatcggggt gagccagttt
     3601 gcggtaaatt ttttcgatgc tttcacggct gttttccaac cagccttctt caaaagaaaa
     3661 gacgccgtga attttgcctt tgaaaatata aatgagggcg gcattgtcga tgccttcatt
     3721 gagaatgatg gtgccggg
//
LOCUS       DAOQNZ010000204         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11621_length_3735_cov_4.239130, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000204 DAOQNZ010000000
VERSION     DAOQNZ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11621_length_3735_cov_4.239130"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PKW73_04945"
     CDS             <1..1296
                     /locus_tag="PKW73_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72659.1"
                     /translation="QKPATQPEKPLHLEPSDFAPQPIETTWETPKKPVAVPLEKPAAA
                     TPEKPAAVGPQNQAAAPSEKPAPAKPVETANLPPQANEKPAEISDKERAEAEKRIIAE
                     VADQIRDGRIKTFDRFYENESIRSERASRTLDQTGTSQDKAREMLGYDDMNLINFNSF
                     KKQVEDQYGFWNNFNSRSYIKSKEDVVSNSRDEQWANLKKLAADQNDPNYKHKQVLLA
                     EIARGMHLPPKEGERVSYYHDDRMATGNQIIAPRAAWQKQAAETLAAIAADPKADHEV
                     VARVLPEIMANPFIPAETRIAAMKGMVALAGDTTSSFMDVRTKAEKQAGNNLAVVGTP
                     REVAIASILKAMHRDNNHYTGKPNAEVQKAGMEALLQLKASETLMQLHSLGKRSPSPE
                     VRQAAEDYSKKIATASQPDLLKLAEMELRRQMQPQAPGK"
     gene            1391..2302
                     /locus_tag="PKW73_04950"
     CDS             1391..2302
                     /locus_tag="PKW73_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72660.1"
                     /translation="MSKCRFRLSFVSALLALACGSNGALAQPADFLDDYYENRLKPAE
                     FKRTIKDLNAQVEKAPQDFNLRMKRGYVQAFARHWEAALGDFTAANKISPSRYEPIRA
                     QAWLMASMNRLKNALTLYNQAIEMAPKNGKLYFERGLVQANIPNFPASIEDSDRAIAL
                     GYKERNVYIQKAKSTFYEGDAEKAVKILDSIPGGKQPQKREYDLKTDFLLVLGRYDEA
                     LKTAEKRLAIPPANVGALLKVAECQIKLKRYKDALANLTKAIALHPTTADPFIRRAAV
                     YRKLGMNSQAEADQKMVEKINSKRHDL"
     gene            2938..3531
                     /locus_tag="PKW73_04955"
     CDS             2938..3531
                     /locus_tag="PKW73_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNA72661.1"
                     /translation="MTTPQTQPETAPNPPPQPEKTKLLDRMIFETRWVLYPMNAGLMV
                     ALTIYLLKFLYQVFILLGHAPALITTTSAEHDHELLVIIVSLLDQAMICSLLIMTIVG
                     GHQIYVRRFQEQLSNQGPYWLRRVDTIVLKVKLGLAFTGVSSVVILKDCISSAVVPTQ
                     VWVTHVVIHAVFLGTTLVTAIVWRIMHPHDSQDKSAK"
BASE COUNT         1041 a         1081 c          895 g          718 t
ORIGIN      
        1 caaaagcccg ccacccaacc ggaaaagcct ctccatctag agccctccga cttcgcgccg
       61 caaccgattg agacaacctg ggagacaccc aagaaaccgg tagccgtgcc actggaaaaa
      121 cctgccgccg ccacccctga aaagccggcc gcagtcgggc cccagaacca ggctgccgcg
      181 ccttctgaaa agcctgcccc ggccaaaccg gtggaaactg ccaatttgcc gccgcaagca
      241 aacgaaaagc cggcggaaat ttccgataag gagcgagctg aagccgaaaa gcgcatcatc
      301 gccgaagtag cggatcagat cagagatgga cgcataaaga ccttcgaccg cttctacgaa
      361 aacgagagca tccgtagcga aagagcctcc cgcaccctgg accagaccgg tacaagccag
      421 gataaagcaa gagagatgct cggctatgac gacatgaacc tgatcaactt caattccttc
      481 aaaaagcaag ttgaggacca gtacggattc tggaacaact tcaacagcag aagttacatc
      541 aaaagcaaag aggacgtagt atccaactcc agagatgagc agtgggccaa cctgaagaaa
      601 ttggcagcag accagaacga tcccaattac aaacacaagc aggtgctctt ggcggagatc
      661 gctcgcggta tgcatctacc tccgaaagag ggagaaagag tgagctacta ccatgacgac
      721 agaatggcta cgggcaatca aatcattgct ccccgcgccg cctggcaaaa acaagcagct
      781 gaaaccctgg ccgccatagc ggccgacccc aaagccgatc atgaagttgt ggcccgcgtc
      841 ttgcccgaga tcatggctaa tcccttcata ccggcggaaa ccagaattgc tgctatgaag
      901 ggcatggtag ctctcgctgg tgatactacc agcagtttca tggacgtgcg cactaaggct
      961 gaaaaacaag ccggaaacaa cctggcagtg gtgggtactc ccagagaagt agcaatcgca
     1021 tccattctca aagccatgca ccgggataac aatcattaca ccggcaaacc caatgcggaa
     1081 gtgcaaaaag ccggcatgga agcgctcttg caactgaagg ccagcgaaac tctcatgcaa
     1141 ctgcacagcc tgggcaagcg ctctccttcc ccggaagtga gacaggcagc cgaggactac
     1201 agcaagaaaa ttgctaccgc ctcccaaccg gacttgctaa aactggcgga aatggaactc
     1261 agaagacaaa tgcagccaca ggcaccgggc aagtaatcgg tagatacatc gcttgcatct
     1321 cgtcgaacgt cacacttggc ttccagtgac ggcacttgcc aggccgcttg ctttgggaga
     1381 gactaatgcc atgtcgaaat gtcgcttccg tctttccttt gtttccgccc tgctggccct
     1441 ggcctgtggc agcaacggcg ccctggctca gcctgccgat tttctggacg attattacga
     1501 gaacagactg aagccggccg aattcaaacg caccatcaaa gatctcaacg cccaggtaga
     1561 aaaagcaccg caagatttca acctgcgcat gaaacgcggc tatgtgcaag cctttgccag
     1621 acactgggag gctgccctcg gcgatttcac cgccgccaat aaaatttcac ccagccgcta
     1681 tgaaccgata agagcgcaag cctggctaat ggccagtatg aaccgcctca aaaacgctct
     1741 cactctctac aatcaagcca tcgagatggc ccctaagaac ggcaaactct actttgagcg
     1801 cggtctggtg caagccaaca taccgaattt tccagcctcc attgaagact ccgatagagc
     1861 catagccctg ggctacaaag aacgaaacgt ctacattcag aaggccaagt ccactttcta
     1921 tgaaggtgac gccgagaaag ccgtcaaaat tcttgattcc attccaggcg gaaaacaacc
     1981 acaaaaacgc gagtacgact taaagaccga ctttcttctg gtgctgggtc gctacgatga
     2041 ggctctcaaa acagcagaga aacgcctggc cattccacct gcgaatgttg gcgccctgct
     2101 gaaagtagcg gagtgccaga tcaaactgaa gcgctacaaa gacgccctgg caaatctcac
     2161 aaaggcaatc gccttgcatc ccaccaccgc cgaccccttt atacgaaggg ccgccgttta
     2221 tcgcaaattg ggcatgaata gccaggccga agccgatcaa aaaatggtgg aaaaaataaa
     2281 tagtaaacgt catgaccttt gatgctgcaa gaaagttgca acaatcaagc cttactatgt
     2341 agccatgaag agaaacgcac accgctcttc aaatgttttt ctgagggggt agctgaaggg
     2401 gagtagtcct tatccttagg gataacggct ctcctctttc agtcatttaa gggcttgcaa
     2461 gcaatacgtg ccacggaaag tatgagacga gtattacaaa tacagccgcc ccgaccgcct
     2521 catgttcaca tgctattcac agaaatctcg ccggaaaaga cagaaaaaca gtaaccggct
     2581 gacagagggc aatgccagtg ccagcgggca cttacagctt tccaaaagct gcgctaacta
     2641 gcgtgatatg agtttggcga agttgccttc acaaatgatg caaatacttc ttttagaggc
     2701 gaatcgacgc tagattcggg aattgtcttt caccttcatt ttctcccgga atccccaacc
     2761 aaaatactaa aaacccccgt ccttccacct tcagccttgc ttcccagagc catcacgtca
     2821 caagtaacaa gcgacgacga tgtccggagc atcctaactt cctcgaactg ccctccagcg
     2881 gatgagccaa gaccatcggc tggatgcagt tcacctattg atgaaatgga gagcactatg
     2941 accactcccc aaacccagcc cgaaacggca ccaaacccgc cgcctcagcc tgagaagacg
     3001 aagctcctgg acaggatgat cttcgagact cgctgggtgc tctatcccat gaacgccggt
     3061 ctgatggtgg cattgaccat ctacctgctc aagttcctct accaggtgtt catccttctc
     3121 ggtcatgcac cggcattgat caccactact tccgccgaac acgatcacga actactggtg
     3181 atcatcgtca gtcttctgga ccaggccatg atctgctccc tgctgatcat gaccatcgtg
     3241 ggcggtcacc agatatatgt acgtcgcttc caggagcaac tgtccaatca gggaccttac
     3301 tggctgcgcc gcgtcgacac cattgtgctg aaggttaagc tggggcttgc cttcaccggt
     3361 gtctccagtg tggtgatctt gaaagactgc atcagttccg cggtcgtacc cacgcaagtc
     3421 tgggtgacac acgtggtcat ccacgccgtc ttcctcggta ccacgcttgt cactgccatc
     3481 gtctggcgca tcatgcaccc ccacgacagc caggataaga gcgctaagta acggtccggt
     3541 gaaagacaac tagcaatttt gagagcggac cggccttccc ggctggtccg ctccttttgg
     3601 gaaattccga ggcatgcaag aaattatgga taatttcgtt caaaccttgc aatgcggccg
     3661 agaatgaatg aaaaacgaga gaacgtattg cgtcaaatta aatccgtacc cgaggccaac
     3721 tgttgcgtca gaaag
//
LOCUS       DAOQNZ010000205         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11625_length_3735_cov_2.971739, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000205 DAOQNZ010000000
VERSION     DAOQNZ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11625_length_3735_cov_2.971739"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..541
                     /locus_tag="PKW73_04960"
     CDS             50..541
                     /locus_tag="PKW73_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72662.1"
                     /translation="MPNYNTSSLALARLAALAVAFPALAGAVALSPALAADPVILPAG
                     TEVARAKDPLAVYKEAGIDLEQERKIRKFAKEFEDQQRVRLKLMANLLKEMRQLELLP
                     DPDEKKALDKQVEINKVQNEISIERIKLLLRIRNVMTFEQKERLVSLIQKNLSGAAPT
                     AVK"
     gene            complement(550..1191)
                     /locus_tag="PKW73_04965"
     CDS             complement(550..1191)
                     /locus_tag="PKW73_04965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNA72663.1"
                     /translation="MAEELHPLEEVTTIYFVRHGHTMATEMGLLYSDPSLEITEKGKL
                     QAQAAARYIAAHRPELLLRGKAVRVAQSAQPIEEILDLQAETIEGFEEWNVGNWEGRT
                     YLDIKKNDPDQYHQWCADPIENAPPEGESIAQLAGRIGEAMQAVSGNTRYAGKKICMV
                     THSGIIRSALVHALEMPVRNFWRFSVPTGSITRIDFSKNFATVQFASVRPELT"
     gene            complement(1233..1934)
                     /locus_tag="PKW73_04970"
     CDS             complement(1233..1934)
                     /locus_tag="PKW73_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNA72664.1"
                     /translation="MKPLNEYKARAELIEVARSAYDRGYICGTEGNFSIRLDSRRILT
                     TPSGACKARLSPSDLLVTNLEGELEPESLKQSKKPSTELRMHLQVYELRADVRAIVHA
                     HPTTAVAFTVAGETLDLPILPEGILALGAVPVAPYATPSTEEVPESIREAARTSNCLM
                     LDHHGALTMGASIHEAYYLLETLEHYAKTLLTAKQLGGARPLTKEQVEKLFAICHVYG
                     IKPPSSAQTILNAAK"
     gene            2073..>3735
                     /locus_tag="PKW73_04975"
     CDS             2073..>3735
                     /locus_tag="PKW73_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNA72665.1"
                     /translation="MRCPECTSRNSVAALKCTQCGARFKRKPLPMKLIGIAGAVVVLA
                     VVVLIGLAMMPAMMNPESNLQKVAKHVANGPKSAQDAERIRAELEDSVKRYLEKNTEL
                     STKEIGDKLRGIFPSAAYEINCFDLPRNLKLVEIDTVLQPSNYLISGKHVTVLRGFEV
                     FDGAKAIEDPTGPVLAILGHKNVQSGRHPQLRLFALLPDGVKERPSDTVPSFTGEGSA
                     AFAKNGKDVELELSVMSRAAEEGLFTMSALASSGLKDEFVKARLNFVGGKYEFVDNNG
                     KSALAALRAAAFAVADPREKGRFSGYLSPTALTAIDQVGKLRTCPPEFELKRLGATAA
                     VTQTAAKPQQQSRRSRRRSRRHRNEDAPAQTQPAPIVVSGPGNRFLMANTDDALEVTV
                     APAGGRFQVTALKRSKSTAAVVATSAASDPGQAVPYEDKTSNLVDKLLSAPDPVLPSA
                     QQMKHTAVLPAVGSAPAPVVQSDEPKVVEKQIAGEAAAIDTSSPSVKVRRGPSTGYRS
                     ITEIKKGQNVEVIGKQDGWYKVRVNGKEGFVYGAFVNCKTSDAYTT"
BASE COUNT          837 a         1034 c          998 g          866 t
ORIGIN      
        1 gaagtcttga gattaagtga agagaagcaa gctcaaatag gtcagcgccg tgccaaacta
       61 caatacctcc tcccttgccc ttgcccgcct ggcggcgctg gctgtcgcat tcccggctct
      121 agccggtgca gttgccttga gccctgctct ggcggcagac ccggttatct tgcctgccgg
      181 caccgaagtg gctcgcgcta aagacccttt ggccgtctac aaagaagccg gtatcgatct
      241 tgaacaagag cgcaaaattc gcaagtttgc aaaagagttt gaagaccagc agcgtgtcag
      301 gctcaaactc atggccaatc tgctcaaaga aatgcgccaa cttgaactgc ttcccgaccc
      361 tgacgagaag aaagctcttg ataagcaggt ggagataaac aaagttcaaa atgagatcag
      421 catcgagcgc atcaaattgc ttctgcgcat tcgcaatgtc atgacttttg aacaaaagga
      481 acgtctggtt tccttgatcc agaaaaacct gtccggggcc gctccgacag ccgttaagta
      541 aggctttgcc taagtcagct ctggtctcac cgaggcgaat tgcacggtgg caaagttctt
      601 actgaagtca attcgtgtaa ttgaaccggt gggtacgctg aaacgccaga aatttctcac
      661 cggcatctcc agggcatgta ccagagccga acgaattatg cccgaatgag tgaccataca
      721 aattttcttg ccggcatagc gagtattgcc gctcacggct tgcatggctt ccccaatgcg
      781 accggctagc tgcgcaatcg actcgccttc aggcggggcg ttttcaattg gatcggcgca
      841 ccactggtga tactggtccg ggtcgttttt cttgatgtcc agatacgtgc gaccttccca
      901 gttgccgaca ttccattctt cgaaaccttc gatggtctca gcctgcaggt ctaaaatttc
      961 ttcgataggc tgagctgatt gagccaccct tacagccttg cctctaagta agagttccgg
     1021 tctgtgggcg gcaatgtaac gggccgccgc ttgtgcttgc agcttgccct tctctgtaat
     1081 ttccaggctc gggtcgctat agagtagccc catctccgtg gccatggtgt ggccgtggcg
     1141 cacaaaataa atggtagtta cttcttctaa tggatgtaat tcttcggcca tttttgaggc
     1201 aggtcctgct ttgtttgttt gactacgatc gactactttg cggcattgag gatcgtttgc
     1261 gccgaactcg gcggtttgat tccgtagaca tggcagatag caaagagctt ttcaacctgt
     1321 tcttttgtga gcggtctggc gccacccaat tgttttgccg tcaatagagt tttagcatag
     1381 tgctccagcg tttccagtaa ataataagcc tcgtggatgc tcgctcccat tgtcagagcg
     1441 ccgtggtgat caagcatcag gcaattggat gttctggccg cctcacggat actctccggt
     1501 acttcctcgg tacttggtgt tgcatatggt gctacaggca cggcgccaag ggcgagaatg
     1561 ccctccggca aaatgggtaa atcgagggtc tcacctgcca cggtaaaggc tacggccgtg
     1621 gtgggatggg catgcacaat ggccctgaca tcagctctca gctcataaac ctgcaggtgc
     1681 attcgcaatt cggtggaagg ctttttactt tgcttgagac tctcgggctc aagttctcct
     1741 tcaagatttg ttaccagtaa atcagaggga cttaatctag ccttgcaggc accactcggt
     1801 gttgtcagta tacgtctgga gtcaagcctg atgctgaaat ttccctctgt gccacagatg
     1861 tagccgcggt cataggccga ccgggccacc tctatcagct cggctctagc cttatattcg
     1921 tttaatggct tcaatgaatt catcttttaa atccgctatt catgagtatt atatacagtc
     1981 gcccagtcat caagcggcgt ccgccaatgc catagaccga gccgacaggc ggtaagtcaa
     2041 aacaggaaat cgtaaacggt cgaacttcaa ctatgcgttg tcccgaatgt acttctagaa
     2101 actcagtcgc tgccctcaag tgtacccaat gcggcgcccg cttcaagcgt aaacctctgc
     2161 ccatgaagct tatcggcatc gctggggcag tggtcgtatt ggccgtggtg gttctgattg
     2221 gtctggccat gatgcctgcc atgatgaatc ccgagtccaa tttgcaaaaa gtagccaagc
     2281 acgtggctaa cggtcccaag tcagcccagg atgccgagcg cattcgtgcc gagttggaag
     2341 actccgtcaa gcgctatctg gagaagaaca ccgagctttc caccaaggaa ataggcgata
     2401 aactgcgggg catcttcccc agtgccgcat atgaaatcaa ctgtttcgat ctgccgcgca
     2461 atctgaagct tgtggaaatc gacacggtac tgcaaccgtc caattattta atcagcggca
     2521 agcatgtcac ggtgctgcgt ggtttcgagg tcttcgacgg ggccaaggct atcgaggatc
     2581 ccaccggacc ggtgctggcc atcctcggac acaagaatgt tcagagcggt cgtcatccgc
     2641 aattgcgtct ctttgccttg ttgcctgatg gtgtgaaaga gcgcccgtcc gacacggtac
     2701 cctccttcac cggggaaggt tcagccgcct tcgccaagaa cggtaaggac gtggagcttg
     2761 agctttctgt catgtccaga gccgctgaag aagggctatt caccatgtcc gcccttgctt
     2821 cttccggtct gaaggacgag tttgtcaaag cccgactgaa ctttgtgggc ggcaaatatg
     2881 agtttgtgga taataacggt aaatccgccc tggctgccct gcgagccgct gcctttgcgg
     2941 tggctgaccc gcgtgagaag gggcgctttt ccggctacct gagtcctacc gccctgaccg
     3001 ccatagacca ggtcggcaag ctgagaactt gtcctcccga gttcgagctg aagcggctcg
     3061 gcgctaccgc tgctgttact cagaccgctg ccaagccgca gcagcagagc cgtcgcagtc
     3121 gacgcagaag tcgccgccat cgcaacgagg acgcaccggc acagacgcag cctgcaccga
     3181 tagtggtatc aggaccgggc aatcgtttct tgatggccaa caccgacgat gcattggaag
     3241 tgaccgtggc cccagcgggc ggacgtttcc aggtcaccgc cctgaagcgc tccaagtcca
     3301 ccgccgctgt agtagccacc agtgccgcca gcgatcccgg tcaggccgtt ccctatgaag
     3361 acaaaacttc caacctggtg gataagctgc tctcggctcc ggaccctgtt ctaccttccg
     3421 cccagcagat gaagcatacg gcagtgcttc ccgccgtggg ctcggcaccg gctccggtgg
     3481 ttcaatcgga tgagcccaag gtagtggaaa agcagatagc cggcgaagct gccgctatcg
     3541 acacaagcag cccctctgtg aaagtacggc gtggacccag cacaggctac cgctctataa
     3601 ccgagattaa gaaaggtcaa aacgtcgagg tgataggcaa gcaggacggc tggtataaag
     3661 tgcgcgtaaa cggcaaggag ggcttcgtat acggggcttt tgtcaactgt aagacctccg
     3721 atgcttacac caccg
//
LOCUS       DAOQNZ010000206         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11643_length_3731_cov_3.506257, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000206 DAOQNZ010000000
VERSION     DAOQNZ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11643_length_3731_cov_3.506257"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1289
                     /locus_tag="PKW73_04980"
     CDS             252..1289
                     /locus_tag="PKW73_04980"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA72666.1"
                     /translation="MAPENIADARVLVTGCAGFLGPWVCELLLERGATVVGLDRQYPQ
                     SSRIHELSEKYASSPERNGSGSGKLKLVNVDIEHYQKVFDVIKDNDIQVIFHLAAQAI
                     VGVANENPVPTFASNIQGTWNILEAVRLLRQAGQSIESIVVASSDKAYGDQEVLPYLE
                     DAPMQGRFPYDVSKSCADLITRSYFHSFALPVAVTRCGNLYGGGDLNFSRIVPGTIQS
                     VLAGQAPLIRSDGSPVRDYIYVQDAARANLAIAERLLQDKSIAGQAFNISNDAPVSVL
                     TMVDEILKNMQRSDLKPVVEGRATREIQAQYLSSERIRGLGWSPRYSLAEGLAEAIAW
                     YREHLSVGAQV"
     gene            1296..2285
                     /locus_tag="PKW73_04985"
     CDS             1296..2285
                     /locus_tag="PKW73_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNA72667.1"
                     /translation="MAGRPCFSSFRIIRFLLQAELWLAALLLFVACTPASCAGESYQI
                     RFQHQLTNKENRAVSDLTVVLALPASCESQQIDELKPESDCRIVPQVLQDQYGQSVHV
                     YRVPRIEPGQTLSFGYFCRARFLDLPNRFQASLGQSKGSVAVPESVRSVYTADDERIY
                     NYRSPQIKALAGQFLSRYPDPLERVKAIHYYVASNLKYSMEDNWDSAPTVLARGSGSC
                     SEFSYLFCALCRAGGLPTRFAAGSRLRKALPYEDRVGHRWSEVYLPSLGWVPFDPTLD
                     GRSASKLDYAGRFFQPSLITFHGGGGSNLLGNAYNSTNTKKELLQRSRIFYWR"
     gene            2354..>3731
                     /locus_tag="PKW73_04990"
     CDS             2354..>3731
                     /locus_tag="PKW73_04990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72668.1"
                     /translation="MKEQAKICSKCRRSIISEERRGSLTSFLLSDLSCCCGQRNASGG
                     RLAGDSNAGICRRCGKVVPALSRPGSMTAFFFKDMRCQCKSPLVDGAGRSGDGLNKGA
                     KGLPAGSLATRYGGGELLQKRTAARTAMRTRRAGAVEAAVLLAPGAVIGGCYKLVELA
                     GQGGMGSVYRARHTVLGRQCAVKFLAPSMVSEQSWRMFQNEAKIISALQHHTICQVYD
                     LGLHEGSLPYYAMDFIHGETLAETIDRQGPLSVGAALELFVKVCEGLSYAHRRGVVHK
                     DLKPANIMLTVDGGDEIEVKILDFGISDLGEAWQSRREHSRVKAGAERLSAPDLSEVI
                     GSAAYMSPEQFRGRDIDRRSDIYNLGCALFETLSGVPPFTGDGFEELEAAHRKAEPPS
                     LQSVTGIRFPSGVEAILSKCLEKSPERRYQSVNELSIDMERVLAVKPLQFARGKESEF
                     LAAQVAGEK"
BASE COUNT          761 a          954 c         1109 g          907 t
ORIGIN      
        1 catgaacaac tggcccagca gttacgccaa gatgctcaaa aaaataagac cgcgtttgac
       61 cttgcgtcgg cagactatga ccgacttcga gttgggtgtg ccgctcagca gttttgtggg
      121 caactgattt gtgggcaatt gatccattgt gcctggcacc cgggctcttg ccgtggtggt
      181 ctccccgggg ctggctcggg gctcctcaaa tatggcataa tgcccattgt tgagtgacat
      241 tttgtgagat tatggcgcct gaaaatatag cggatgcacg tgtgttggta accggttgtg
      301 ccggcttcct cggaccctgg gtatgtgagt tgcttctgga gagaggtgcg acggtcgtag
      361 ggcttgatcg tcagtatccg cagtcctctc gtattcatga actgtctgag aagtatgcct
      421 cttcaccaga gcgtaacggc agcggcagcg gcaagctcaa actcgtgaat gtagacattg
      481 aacattacca aaaagtattc gacgtcatca aagacaatga cattcaagtg attttccacc
      541 tggcggccca ggccattgtc ggggtggcca atgagaatcc cgtccccacc ttcgcttcca
      601 acattcaggg tacctggaat attttggagg ccgtgaggct cctgcgccag gccgggcagt
      661 ccatcgagtc catcgtggtg gcttcttccg ataaagctta tggcgaccag gaagtcttgc
      721 cctaccttga agatgcgccc atgcaaggtc gcttccccta tgatgtttcg aagagctgtg
      781 ccgatctgat cacaagatct tattttcatt cctttgctct gcctgttgca gtgacccggt
      841 gcggaaactt gtatggcggc ggggacctta atttcagtcg tattgttcca ggcacgattc
      901 agtcagtgct ggccgggcag gcgccgctca ttcgctccga tggatcgccg gtgcgcgatt
      961 atatctatgt gcaagatgca gccagagcca atctggcaat cgccgagcgg cttttgcaag
     1021 ataagagtat agccgggcag gctttcaaca ttagcaatga cgcgccggtg tcggtgctga
     1081 ccatggtgga tgagatcttg aagaatatgc agcgcagcga cctcaagccg gttgtggaag
     1141 gtcgtgccac cagggagatt caagctcagt atttgagttc ggagcgcatt cgcggtctgg
     1201 gttggtcacc ccgttacagc cttgccgagg gacttgccga ggcgatcgcc tggtacagag
     1261 agcatctcag tgtcggagcc caggtttaag tctgaatggc tggtcgcccc tgcttttcga
     1321 gcttccgcat aattcgcttt cttttacagg cagaactctg gctggccgct ctcttgctct
     1381 ttgtggcttg tactccggct tcttgcgccg gggagagcta tcaaattcgc ttccagcacc
     1441 agttgaccaa taaagaaaat agagccgtca gtgatctgac cgtggtgctt gcccttcctg
     1501 ccagctgtga aagccagcaa attgacgaac tcaaaccgga gagcgattgc cgaatagtcc
     1561 ctcaagtttt gcaagatcag tatggtcaat cagtgcatgt atatcgggtg ccgcgcatag
     1621 agccgggaca gaccctctct ttcggttatt tctgccgggc ccgctttctg gacttaccga
     1681 atcgtttcca agcttctctc gggcaaagta aaggctccgt cgccgttcct gagtcggtgc
     1741 gttccgtgta caccgcggat gatgagcgca tttataacta ccgttcgcca cagattaaag
     1801 ccctggccgg tcagtttctc tcaaggtatc cagatccgct ggagcgggtg aaggcaattc
     1861 attattatgt ggccagcaat ctcaaataca gtatggaaga caactgggac agcgccccga
     1921 ctgtactggc tcggggctcg ggcagctgtt cggagttcag ctatttgttt tgcgcgctct
     1981 gtcgggctgg tgggctgccc acacgttttg ccgccggctc caggctgaga aaggcgctgc
     2041 cctacgagga tagagtgggg caccgctgga gtgaggtata tcttccctcc ctgggttggg
     2101 tgcccttcga tccgacactg gatggtcgca gcgccagcaa gcttgactat gccgggcgat
     2161 tttttcagcc ttccctcatt acctttcacg gaggcggcgg tagtaatttg ctcgggaacg
     2221 cctacaacag cacaaacact aagaaggagc tcttgcagcg cagtcgtatt ttttactggc
     2281 gctgacatgc agcgcaggcc gatggtcttg cgctggactt cgccggactg attaggttgt
     2341 atgattagat tgcatgaaag agcaggcaaa aatttgttca aagtgccgca gaagcataat
     2401 ttctgaggag cggcgtggtt cgcttacctc atttttgctc tccgacttga gctgttgttg
     2461 cggtcagaga aatgcctccg gcggtcgcct ggccggcgat tccaacgccg gtatctgtcg
     2521 gcgatgtggc aaagtggtac cggccttgag ccgccccggc agcatgacgg catttttctt
     2581 caaagatatg cgctgtcagt gcaagtcgcc gctggtggac ggtgccggtc gtagtggtga
     2641 cgggcttaat aagggcgcta aagggctgcc cgctgggtcg ctggccaccc gctatggcgg
     2701 cggcgaactt ttacagaagc gcactgccgc ccgcacggcc atgcgaactc gccgggcggg
     2761 ggccgtggag gctgctgtct tgctggcgcc aggcgcggta attggcggct gctacaagct
     2821 tgtggaactg gccgggcagg gtggtatggg ttcggtttat cgggccaggc acacggtatt
     2881 aggccgccag tgtgccgtta agttccttgc accgtcgatg gtgtcggagc agagctggcg
     2941 aatgttccag aacgaggcaa aaataatctc cgccctgcag caccatacta tttgccaggt
     3001 ctatgatctg ggattgcatg aaggaagtct gccttactat gccatggatt tcatccacgg
     3061 tgaaactctg gctgaaacca tagacaggca ggggccgctt tcggtaggtg cggccctgga
     3121 gcttttcgtc aaggtctgcg aggggctttc ttatgcccac agaagaggcg tggtgcacaa
     3181 agatttgaag ccggccaata taatgctcac cgtggatggt ggcgatgaga tcgaggtgaa
     3241 aattcttgat tttggaattt ccgatctggg agaagcctgg cagagtcgtc gcgaacattc
     3301 tagagtgaaa gcgggggctg aaaggctttc ggcgccggat ttgagtgaag taatcggtag
     3361 tgccgcttac atgagccccg agcagtttcg agggcgcgac attgaccgcc gctcggatat
     3421 ttataatttg ggctgtgctc tctttgagac cctgagcgga gttccaccct ttaccggcga
     3481 cggttttgaa gaactggagg ccgcccatcg caaggcggag ccgcccagtc tgcagtctgt
     3541 aaccggcatc aggtttccct ccggtgtgga agcaattctc agtaagtgtc tggaaaaatc
     3601 gcccgagcga agatatcaga gtgtcaatga gctttccatc gacatggagc gtgttctggc
     3661 tgttaagcca ttgcagtttg cccgcggtaa ggagagcgag tttctcgctg cccaggttgc
     3721 cggggagaag g
//
LOCUS       DAOQNZ010000207         3729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11658_length_3729_cov_2.864181, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000207 DAOQNZ010000000
VERSION     DAOQNZ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11658_length_3729_cov_2.864181"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PKW73_04995"
     CDS             <1..938
                     /locus_tag="PKW73_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNA72669.1"
                     /translation="KGGLIGVGNRFYRGAILVRVNKRANATILSAINIVDLEDYLLSV
                     VPSEVPSRWPQEVLKAQAIAARSYAVANLNKHKAEGYDVKDTVDDQVYLGVQSESEET
                     NQAVSATSGLVLKHNQNVISAFFHSTSGGTTELAETVWGKSLPYLKSVVDYDDESPHF
                     NWKREVKADKLSSLFGAAPKSAPLVSDGAPVRKERIVALLVVDRHGGDGRRVKNLLVV
                     GEEKIRLLTGSEARRLYNLPSTQFSVFQNGDTYIFSGRGFGHGLGLSQWGAKALADRG
                     YNAGQILSYYYKDVTIESLAPKELNDARALQNGAI"
     gene            960..2114
                     /locus_tag="PKW73_05000"
     CDS             960..2114
                     /locus_tag="PKW73_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA72670.1"
                     /translation="MFEQFLFILLMLGVLSVLIIVHECGHYLVARFFGFQTPVFGIGL
                     PFGPHVVVGQRWGTEFRIYACLLGGFVAIPELGDETNAGASSEAFGVDLNPFKKFPIW
                     QRALVAVAGVTFNVIFAYFLMVVMLLALGRPIDMTYVRQLDPGNPIARDAGVLANDEI
                     LAIDNHPVTNAEEIIAYLGSKKSTQVTLNLRRDGQPVSLTMTTSPRGKVGMVLDAKAT
                     QYEKVEGNFLDVCVLGGQELTNRTRLMVVKIVELVQNLLPDFGPKQPGAAPKASIKDL
                     HGVLAVIKIGADMANVDWRTLFMFTILISMDLAIVNLLPWPALDGGHLAFMLVEAIRG
                     KPIEERAHGEMVRWGFLSLIALMVIVMVNDVSALMSGQLDLKPHLKQKGK"
     gene            2128..2379
                     /locus_tag="PKW73_05005"
     CDS             2128..2379
                     /locus_tag="PKW73_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNA72671.1"
                     /translation="MPSYDYRCQGCDSNFTVERSMTASTETSCPQCQSEKVSRIWNMN
                     FRTGGCTPDVGQGTTKSSSSSGGGCGSCSSHSCGSGSCH"
     gene            complement(2489..3376)
                     /gene="panB"
                     /locus_tag="PKW73_05010"
     CDS             complement(2489..3376)
                     /gene="panB"
                     /locus_tag="PKW73_05010"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNA72672.1"
                     /translation="MSKAVSTLTLQKYKKDGRKIVALTAYDYSMAKILDRAGVDLILV
                     GDSLAMVALGHRTTHAVTMEEMLHHTRAVTRGVSTAMVVADLPFMSYQVDVTQAIMNA
                     GRFVKEAQAHAVKLEGATRLNLEIIERLVGMGIPVMGHLGYTPQAIHGLGGARVQGRS
                     ADQAIKLLDDARALEMAGCFSVVLEMVPTAVAKMVTEKLNIPTVGIGAGNACDGQILV
                     TDDLLGKFTDFLPRFVRRYADLSTVCQEAVASYAADVKSGDFPNASESFLLPKDEEAA
                     LNKQLQLDKSVPAGSTEIC"
     gene            complement(3376..>3729)
                     /locus_tag="PKW73_05015"
     CDS             complement(3376..>3729)
                     /locus_tag="PKW73_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72673.1"
                     /translation="EHGEVDSAAQSFAKAAELDPRCFVAFQNWGAVLEAQGDLRGASE
                     KYQAALDLKPKYDGLYYNMAYLLEKLNMRSEAGRWYKRFHELAGKYPYDPKHIVSLQQ
                     EEARERARSAASRKN"
BASE COUNT          806 a          990 c         1015 g          918 t
ORIGIN      
        1 agaagggcgg gctgattggc gtgggcaatc gtttctaccg cggcgccatt cttgtgcggg
       61 tgaacaaacg agccaatgca acgattttga gcgccatcaa tatcgttgat ctggaagact
      121 atttgctttc ggtagtaccg tcggaagtgc ccagccgctg gccccaggaa gtgctgaaag
      181 cccaggccat agcggctcgc tcttatgccg tcgccaattt gaataagcac aaagccgagg
      241 gctacgacgt caaggacact gtggatgatc aggtctacct gggggtgcag tcggaaagcg
      301 aagaaaccaa ccaggcggtg tccgctacct cgggtcttgt gctcaaacat aatcaaaatg
      361 tcatcagcgc tttctttcat agcactagtg gtggtactac cgaactggcc gaaacagtct
      421 ggggtaagag tttgccgtac ttgaaatcgg tggtcgatta tgacgatgaa tcaccccatt
      481 tcaactggaa aagagaagtt aaagccgata agttgtcttc cctcttcggg gcggctccca
      541 agtcggcgcc gctggtttcc gatggcgctc ccgttcgtaa agagcgaatt gtcgcccttt
      601 tggtggtaga ccggcacggg ggcgacggca ggcgcgtcaa gaacctcctg gttgtagggg
      661 aggagaagat ccgtttactc accggcagcg aggccagaag gctctataac cttcccagca
      721 cacagttttc agtctttcag aacggcgata cctatatttt cagcggtcgc ggctttgggc
      781 acggtcttgg tctttcgcaa tggggagcca aggccctagc cgatagaggc tataatgcag
      841 gacagattct ttcgtattac tataaagatg tgacgattga gtctcttgcg ccgaaggaac
      901 taaatgacgc aagggcgctc caaaacgggg cgatttagcg gtttagtgag gttatctcgt
      961 tgttcgaaca gttcttgttt atcctcttga tgcttggcgt tctgagtgtc ctcatcatcg
     1021 tgcacgaatg cggccactat ctggtggccc gcttcttcgg ctttcagacc ccagttttcg
     1081 gtatcggtct accctttggt ccccatgtgg tggtgggcca gcgctggggt acggagtttc
     1141 gcatctatgc ctgcctgttg gggggcttcg tagccattcc cgaactgggt gatgaaacca
     1201 atgccggagc ctcttccgag gcattcggcg ttgatttgaa tccgttcaag aaatttccca
     1261 tctggcaacg agccctggtg gctgtggctg gtgttacttt taatgtcatt tttgcttatt
     1321 ttctaatggt cgtcatgctc ctggccctgg ggcgcccgat tgacatgacc tatgttcgcc
     1381 aactcgatcc aggcaatccc attgccaggg atgccggtgt gctggccaat gacgaaatcc
     1441 tggccattga taatcaccct gtcaccaatg ccgaagagat cattgcttat ctgggttcca
     1501 agaaatccac ccaggtgacg ttgaacctgc gccgcgacgg ccagccagtc agtttgacca
     1561 tgaccaccag ccccagaggc aaagttggta tggtgcttga tgccaaggct acgcaatacg
     1621 agaaggtcga aggtaatttc ctcgatgttt gtgtgctggg cggtcaggaa ctgaccaatc
     1681 gcacccgttt gatggtagtg aagatagtag agctggtgca gaacctgctc cccgattttg
     1741 gaccgaagca gccgggggca gcgcccaaag ccagtatcaa agaccttcac ggtgttctgg
     1801 cagtaattaa gatcggtgcc gatatggcca atgtggactg gcgcaccctc tttatgttca
     1861 ctattctcat ttccatggac ctggccatag tcaacctttt accctggccg gctctggatg
     1921 gcggtcacct ggcctttatg ctggtggaag caattcgggg taaacccata gaagaaaggg
     1981 cccacggaga gatggtgcgc tggggcttcc tcagtctgat cgctctcatg gtcatagtta
     2041 tggttaatga tgtttccgct cttatgagcg ggcaactcga cttgaaaccc catctcaaac
     2101 agaaaggcaa ataggaagga taggaagatg cccagttatg attaccgctg ccagggatgc
     2161 gacagtaact tcaccgtgga acgctccatg actgcttcca ccgaaaccag ttgcccccag
     2221 tgccagagcg agaaagtctc acgtatctgg aacatgaatt tccgcaccgg cggttgcacc
     2281 cctgacgttg gtcagggcac cactaagtcg agcagctctt cgggcggtgg ctgtggctct
     2341 tgcagcagcc attcctgcgg ctccggttcc tgtcactgat actgaagtcc gccggtttct
     2401 cccgataata ctgaagtccg ccggtttctg tcactgatac tgctagaggc agcccacttc
     2461 tgtcacagat actgaaggca ggccgcttct aacagatttc cgtgctgccg gccggtacag
     2521 atttatccag ttgcagttgc ttattgagcg ctgcttcctc gtctttaggc aagagaaaag
     2581 actctgaggc gttagggaag tcacccgact tcacatcggc ggcatagctt gctacggctt
     2641 cctgacagac cgtagaaaga tcggcgtagc gcctgacaaa gcgcggcagg aagtcggtga
     2701 acttgcccag aagatcgtcg gtgaccagga tctgaccgtc gcaagcattg cctgctccaa
     2761 taccgacggt agggatgttc aatttttccg tcaccatttt ggccacggcc gtaggcacca
     2821 tttccagtac caccgagaaa catccggcca tctccagagc acgggcgtca tcgagaagct
     2881 tgatggcttg gtcggctgag cgtccctgta ctcgggctcc acctaaacca tggatagcct
     2941 ggggcgtata gcccaggtga cccataaccg gaatgcccat gcccacaagt ctttctatta
     3001 tttccagatt gagcctggtg gctccttcca acttgacggc gtgggcctgg gcttctttca
     3061 cgaaacgacc ggcgttcata atggcctggg tgacatcaac ctggtagctc atgaagggaa
     3121 gatcggctac aaccatggct gtgctcacac ctctggtcac ggctctggtg tggtgcaaca
     3181 tctcttccat ggtcacggca tgagtagtgc ggtgtcccag agccaccata gccagactgt
     3241 cgccaaccag aatcagatca acaccggctc ggtcaagaat tttcgccatg gaataatcgt
     3301 aggctgtgag agcaactatt ttgcgcccgt ccttcttgta tttctgcagg gtgagagtgg
     3361 atacggcttt agacattagt tcttcctgct tgcggcactt ctagctcttt ctctggcttc
     3421 ttcctgctgt aaagagacta tgtgtttggg atcgtagggg tacttgccgg ctaattcatg
     3481 gaagcgcttg taccaccggc cggcttcact gcgcatattt agcttttcca gaaggtaagc
     3541 catattgtaa tagagaccat cgtacttggg cttcaagtct aatgcagctt gatacttttc
     3601 tgaggctcct ctcaagtcgc cctgggcttc cagcaccgct ccccaattct ggaaggccac
     3661 gaagcacctg ggatcaagct cggcggcttt agcgaagctc tgagcggcgc tatcaacctc
     3721 gccgtgctc
//
LOCUS       DAOQNZ010000208         3722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11692_length_3722_cov_4.424870, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000208 DAOQNZ010000000
VERSION     DAOQNZ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11692_length_3722_cov_4.424870"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1076)
                     /locus_tag="PKW73_05020"
     CDS             complement(132..1076)
                     /locus_tag="PKW73_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA72674.1"
                     /translation="MEDVGAAEPLYRVIRKIRRPQSLRELMAACLRSIYMGDPFTRES
                     DALPELPERLRKAVTLEECLIAGIRCQVYRPTGRDEPLPLLIYMHGGGFVIGCSEDTD
                     YITRMLCDLSQVVVVSINYRLAPETVFPGAILDCERVLESVLNESLKFSVAADCVYLA
                     GDSAGANLALALWARLGKRQNQVKGMVLLAPWLDMEVEKYESYNRLAPEGIVFDAAFI
                     GYARAAYVGFEKWRHPQVSPIYSPLLELPPTIVLIGEEDPFIDQALAFKERARVVGSQ
                     KLEVKVFAGMPHCFYSFPGLFEEEKACYTAIAEFLARA"
     gene            complement(1094..2734)
                     /locus_tag="PKW73_05025"
     CDS             complement(1094..2734)
                     /locus_tag="PKW73_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72675.1"
                     /translation="MLTDVLIDLAGRIFSGWEGRVVLALSLTLTVPAFAASPTTDAVL
                     KPIDPSAAQLDTPPAPEPAPGSTGGANPFNSNIQGQPLGRGDKAGFGGSGLNMETPQE
                     DASPQDELRIAGLEQKAFGTTYNEHEVVDRLDHLEKEVFGSAKTGSLAERLGRLETKL
                     LGGSAFGAGAGRPMQPPPPVQGFPPQQLPPQGGSGMNQGQYRPPVNSPPPYREPQPYN
                     QGSQGNQGNYQPPNQRPSYPPSGYQQPGFPQGNFPQGNFPPQNQGGNNSQYQNYGQPP
                     VSYQNQIPPPVQRPPANFAPFPPGNPQGNPPGNQMGFPPGAPQAVGGFSLDASMVIAG
                     LPYDPVVGDYLPAIRKFPGAGGGISYAQWRKFPVRVRVPQGSPDSWNRSIEAVIARWG
                     QYVPVKIALPKEDANAEIIWVNTLPKGLLGVTRFGIKDGQMRTWVYLLRPSFYPPEIP
                     ERSLSPVFMREMGHVLGLLGKSDKNSDIMYQAESTAAKAPSKTAPPAGARAQASLVNL
                     KIAPISTRDINTLKRIYQSPPLPDNLTLNEPMEWATSY"
     gene            complement(2728..>3722)
                     /locus_tag="PKW73_05030"
     CDS             complement(2728..>3722)
                     /locus_tag="PKW73_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72676.1"
                     /translation="EGGPTGDLYYNEIQYLVINGRVSTAEHELVGIMDDNLNLAVRDH
                     YTRTPFRRVDENNMLSFYFDGVTSAGKAIKKDFLKPLYRRDKPYFENEIIRYFQDFDK
                     LGVQQKKYVLDSLGIWAKSGLLQVVRKSEGNCAMGNVKHGAAGVTRVRTGMVDLDAAE
                     FDRDIDLFKRFGALASVSSPFPPHVEVRVNLVVSHEFGHQLQFCLSQASQDRIAELYQ
                     GRLTRSRRTNPAPHGYEGRSELLRPENVMSRLFISGYSATSKEEYWAEAVAAFSVPES
                     RKILNDMDPAICQILTEILYHPEQHLSAVLVEQILDLQASLRAGKALPEDLLTC"
BASE COUNT          891 a         1028 c          987 g          816 t
ORIGIN      
        1 aaaactacgg ctgcgtatct cttcatacga tcacaaaaat agcagccgta cttcaagcag
       61 aggatttgac ttagctttgc caactcacta agctatgcgg caaactaacc gcaatctatc
      121 taaacaggcc gtcatgcccg cgcaagaaac tcggcgatgg ctgtataaca tgctttctct
      181 tcctcaaata atccagggaa agagtagaag cagtggggca tgcccgcaaa caccttcacc
      241 tcaagctttt gagagccgac cacccgggct ctttccttga aggcaagagc ctgatctatg
      301 aatgggtctt cttcgccaat cagtactatg gtcggtggaa gttcaagtaa tggggaataa
      361 attgggctca cctgcgggtg gcgccatttc tcaaaaccca catatgcagc ccttgcatac
      421 cctataaaag cagcatcaaa gacaatccct tccggtgcca accggttgta ggactcgtat
      481 ttttccactt ccatatccaa ccagggagcc agcaagacca tgcccttaac ttggttctgc
      541 cgttttccca gtcgggccca cagggctagg gcaaggttag ctccggccga atcaccggca
      601 aggtaaacac agtctgcagc aacactaaat ttgaggctct cgttcaaaac actttccagg
      661 acacgctcac agtcaagaat cgcacccgga aaaaccgttt cgggcgccaa tcgataattg
      721 atactgacta caactacctg acttagatcg cacaacattc gcgttatgta atcagtgtcc
      781 tcggaacacc caattacaaa ccctccccca tgcatgtata tcagcagtgg cagtggctca
      841 tcacgaccgg ttggacgata gacctgacag cgaattcccg cgatcaggca ttcctctaga
      901 gtaactgctt tccgcagccg ctcaggaagc tcaggcagtg cgtcactttc cctggtaaaa
      961 ggatctccca tgtaaatact gcgcaagcaa gcagccatca gttcgcgcag tgactgggga
     1021 cggcgtatct tgcgaataac tctgtacagg ggctccgccg caccgacatc ttccatcaaa
     1081 gccaaaatgc tccctaataa gaagtagccc attccatggg ttcgttcaaa gtcaggttgt
     1141 ccggcagagg cggcgactga tagatgcgct tgagcgtgtt tatgtctctg gtactgattg
     1201 gcgcaatttt caaatttacc agagacgctt gagcacgcgc accggctggc ggtgcggtct
     1261 tgctcggcgc tttagcggcg gtgctttcag cctgatacat gatatcgcta ttcttgtcgc
     1321 tcttcccgag caaaccaagc acatgcccca tttcccgcat aaatacgggc gacaaagagc
     1381 gctccggaat ctcgggcggg taaaaactgg ggcgcagcaa ataaacccag gtgcgcatct
     1441 gaccgtcttt tataccgaag cgagtcacac caagaagccc tttgggcaag gtatttaccc
     1501 agataatttc agcattggca tcttccttag gcagggcaat tttgaccggc acatattgcc
     1561 cccagcgcgc tatcaccgct tctatggaac ggttccagct atcgggagac ccctggggta
     1621 cgcgcacgcg caccgggaac ttacgccact gggcataact gatgccgccg cccgcaccag
     1681 gaaacttacg aatggcaggc aggtaatcac ccaccacggg atcataaggc aaaccggcta
     1741 ttaccatgga ggcatctaaa ctgaagccgc ccactgcctg gggcgctccc ggcggaaagc
     1801 ccatttgatt tcccggcgga ttgccttgag gatttcccgg cggaaagggg gcaaaattgg
     1861 caggcggtct ttgcaccggc ggtggtatct gattttgata gctgaccggt ggctgcccgt
     1921 agttttgata ctgtgaatta ttaccgccct gattttgagg agggaaattg ccctggggaa
     1981 aattaccctg aggaaagcca ggttgctggt agccgctggg tggataactg ggtctctggt
     2041 tcgggggctg atagttgccc tgattaccct ggctgccctg attatatggc tggggctcac
     2101 ggtaaggggg cggagaattc acaggcgggc ggtattgccc ctgattcata ccgcttccac
     2161 cctggggcgg caattgttga ggtgggaaac cctgcaccgg cggcggtggc tgcatcggtc
     2221 ggccggctcc agcgccgaaa gcgctaccgc ccaataactt cgtttcgagg cgacccaatc
     2281 gttcagccag agaaccagtc ttggcagaac caaagacctc tttctccagg tgatcaagac
     2341 ggtctaccac ctcgtgttca ttgtaggtgg taccgaaggc tttctgttca agcccggcga
     2401 tacgcagttc atcctgagga gaggcatctt cctggggcgt ttccatgttg agaccggagc
     2461 cgccaaaacc ggctttgtcc ccacgaccaa gaggctgtcc ctggatattg gaattgaagg
     2521 ggttagcacc gccggtagag ccaggcgccg gttcgggagc cgggggcgta tccagttgag
     2581 cggcgctggg gtctatgggc ttgagcacgg catcggtggt gggcgaggca gcaaaagcag
     2641 gcacagtcaa cgtaagagaa agagccagga ccacccgccc ttcccaacct gaaaaaatcc
     2701 gtccggccaa atctataagt acatcagtca gcaagtcagc aaatcctcgg gtaacgcctt
     2761 gccggctcgc agtgatgcct gcaggtctaa aatttgttcc acaagaacgg cggagagatg
     2821 ctgctcggga tgatagagaa tctcagtcag aatctgacag atagccgggt ccatatcgtt
     2881 caaaatcttg cggctttccg gcacggaaaa ggccgccacc gcttccgccc agtactcttc
     2941 cttactggtg gcggaataac cggaaataaa tagtcggctc atgacgtttt ccggtcgcaa
     3001 aagttcggag cgcccttcgt agccgtgggg cgccggattg gtgcgccggc ttctggtcag
     3061 ccgtccctga taaagttcgg caatgcggtc ctgggaagcc tgggaaagac agaattgcag
     3121 ctggtgcccg aattcatggg aaaccaccag gttgactcgc acttccacat ggggcgggaa
     3181 aggagaagag acgctggcca gggcaccaaa tcgcttgaag agatcaatat cgcgatcaaa
     3241 ttcagcggca tccagatcga ccatgccggt tcgaacccgc gtgacaccgg cggcaccatg
     3301 cttgacattg cccatggcac agttgccttc gctcttgcgc actacctgca aaagaccact
     3361 cttggcccag attcccagac tatcgagcac gtactttttc tgctgcacgc ccagcttatc
     3421 gaagtcttga aaatacctga tgatctcatt ttcaaaataa ggtttgtcgc gccgatagag
     3481 cggcttgaga aaatctttct tgatggcttt accggctgaa gtcacaccgt caaaatagaa
     3541 ggaaagcata ttattttcgt cgacgcgccg aaagggcgtg cgcgtgtaat ggtcgcgcac
     3601 cgccagattg aggttatcgt ccataatgcc gaccagttca tgttcggcgg tggagacgcg
     3661 cccattaatg accaggtact gaatctcgtt atagtaaagg tcgcctgttg gcccgccttc
     3721 ct
//
LOCUS       DAOQNZ010000209         3712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11744_length_3712_cov_3.370796, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000209 DAOQNZ010000000
VERSION     DAOQNZ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11744_length_3712_cov_3.370796"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="PKW73_05035"
     CDS             <1..1226
                     /locus_tag="PKW73_05035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNA72677.1"
                     /translation="QGGTVVYTSGFKLDQTPVDGRMLDIGRLASIGDEGVMLLISCSA
                     NVEEGGYSASEKVVASTMERISSSASGRLLVVMPGTNTHRLQVLFDVAQKTGRKLFLQ
                     GETLHKVAVCAAISGHLQYDRAIEGLPAAMHKTADRQVLLLESSKEADPIDVLEAIAY
                     GEHKTVSLKEGDTLVFSSDVVPGEARRMANILDELMANSVHCYFGTRQGVHVSRYGAR
                     EELKLMLSVTKPRYFAPAMGESRHLMQHGQLGEFFDLSEEEIFNLSNGDVLELRNGKA
                     RLYGHVEAEAVLFNRDQGERVTTASVKERQALSLEGVLTVGLTVGESGKLVSGPNLAC
                     GAAGFLLTDDWQEVQKQIAEVVEGTVDRIASERQGDERDSGPYMRSAIRESVVKVLRN
                     RLGAKPTVQILIHQV"
     gene            complement(1238..2119)
                     /locus_tag="PKW73_05040"
     CDS             complement(1238..2119)
                     /locus_tag="PKW73_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72678.1"
                     /translation="MVSKRSVRTSTLALSLSVFLVSFLSLPGLVNQALAAGSSDNAGE
                     FFTEAESAQTKAAAGSNPVAPPPEGAPTRVNAVIKMKVIVPQMKQAQTKFTAFPFTVK
                     PPKLAVPITPLPTEDKGLESMILGSGYASKLSLEKPYPDGGWRWQYAYRNGLKRSGQT
                     PPKVMIGIYNWSRSLVKYVKPECERINEIEAKRMERYKKLLQDYEDNHKDEEMEALRH
                     GHEPLPIKLRPIRRNNQLVSYEASYELPPGEWWITGEHKVPGLVYYWEYPMQIKAGEN
                     INLVMDDANAVLIQGGW"
     gene            complement(2221..3669)
                     /locus_tag="PKW73_05045"
     CDS             complement(2221..3669)
                     /locus_tag="PKW73_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNA72679.1"
                     /translation="MVKGAHDGHAHGQEPAAPASTGEKTAASAVVDLKQMEEELNKRE
                     IGLIEREIKALRLQEELDRLRPLSGLYRVVKAMATERKLDSLLETITRETQAIVKCDR
                     CSVFVLDQDKGELWTQVAQGLVGVRTIRIPLSGTAIVSHCARSGRIINIPDAYQDERF
                     DPAVDKQTGYKTRSVLCVPMRNREGTVIGVFQVLNKLSGPFTSEDEDWLEALTAVAAG
                     LIEQAQAYAEIERFVDKTLEVLAQTIDKRDPLTAGHSVRVTNYSLILGTAMDVVSQEL
                     DVLRYSAMMHDYGKIGVPEEILWKDGRLTPDEYAKVQKHASITYELLINLPFTKRLQA
                     VPYVASCHHEKLDGTGYYRGLKGEEIPFLSRIIAVADVFDALTSVRHYRNRMPVEKVA
                     EILESGKGNHFDGRCVDTFNRMPSDRVLMVMESERDRKASEDLSIFKHVSWQRLVELV
                     SGSKPKRGEDGVRESFDRLYNFGLPEGYKALD"
BASE COUNT          749 a          983 c         1085 g          895 t
ORIGIN      
        1 gccagggggg aacggttgtt tatacctccg gcttcaagct ggatcaaacg ccggtggatg
       61 gtcgcatgct cgatatcggt cgcctggcgt ccatcggtga tgaaggcgtg atgctgctca
      121 tctcctgttc ggccaatgtg gaggaaggcg gttattctgc ttccgagaag gtggtggcaa
      181 gcaccatgga gcgcatttct tccagtgctt cgggtcggct gctggtagtc atgccgggca
      241 ccaataccca ccgcttgcaa gtacttttcg atgtggccca gaagaccggt cgcaagctct
      301 ttttgcaggg ggaaaccctt cataaggtag cggtctgcgc cgccatctca ggacatttgc
      361 agtatgaccg tgccatagaa gggctgcctg ccgccatgca caagaccgcc gatcggcagg
      421 tcttactgct tgaaagcagt aaggaagccg atcctattga tgtgctggaa gccattgctt
      481 acggcgagca caagacagtt tctctcaagg aaggcgatac ccttgtcttc agcagcgatg
      541 tggtgccggg cgaggccagg cgcatggcca atatactcga tgaacttatg gccaattccg
      601 tgcactgtta tttcggtacc aggcagggcg tgcatgtctc cagatatggt gccagggaag
      661 agttgaaact gatgctctcg gtcaccaagc ctcgctattt cgcgccggcc atgggcgagt
      721 ccaggcactt gatgcagcac ggccaactgg gagagttctt cgatctttct gaggaagaaa
      781 ttttcaacct cagcaatggc gacgtgttgg agttgcgcaa tggtaaagcc aggctctatg
      841 gccatgtgga agcggaggcg gtgctgttca atcgtgacca gggtgaaaga gtgaccacgg
      901 cttccgtgaa ggagcggcag gcgttgtctc tggaaggcgt tcttaccgtc ggtctgaccg
      961 ttggtgaaag cggcaaactg gtaagtggtc ccaatctggc ttgtggagcc gccggtttcc
     1021 ttctgactga tgactggcag gaagtgcaga agcaaatcgc cgaagtagtt gagggaacgg
     1081 tggaccgtat tgcctcagaa cgtcagggcg atgagcgaga cagtggtcct tatatgcgct
     1141 ccgccatccg ggaatcggtt gtgaaagtat tgcgcaaccg cctgggggct aagcccaccg
     1201 tgcagatact aattcatcaa gtttagaaga gctggcgcta ccagccaccc tggatcaaga
     1261 cagcgttggc gtcatccatg accaggttga tgttttcgcc ggccttgatt tgcatggggt
     1321 attcccagta atagacgagc cccggaactt tgtgctcccc ggtgatccac cattcccccg
     1381 gcggcaactc gtaggaagcc tcgtaagaaa caagctggtt atttcttctg atggggcgaa
     1441 gtttgatggg cagcggttcg tgtccgtgcc tgagggcttc catctcttcg tctttgtggt
     1501 tgtcttcgta atcttgaagc agcttcttat agcgttccat gcgcttggct tcaatttcat
     1561 tgatgcgttc gcactcgggt ttgacgtatt tgacaaggct tctggaccag ttgtagatac
     1621 cgatcatgac cttgggcggc gtctgtccgg agcgcttcaa gccgttgcga taagcatatt
     1681 gccagcgcca gccgccgtcc gggtagggtt tttccagaga gagcttactg gcgtagccgg
     1741 agcccaggat catgctttcc agacccttgt cttcggtggg caggggcgtg atgggaactg
     1801 ccagtttggg cggctttacg gtgaagggaa aggccgtgaa cttagtctgg gcctgcttca
     1861 tctgcggcac tatcaccttc atcttaatga cggcattgac cctggtcggt gccccttccg
     1921 gcggcggcgc caccggattg ctaccggcag ccgctttggt ttgagcgctc tccgcttccg
     1981 tgaaaaactc gccggcatta tcggaagaac cggctgccag agcctggtta accagcccgg
     2041 gcaggctcaa gaacgacact agaaagacgg acagggaaag agctaatgtg cttgtgcgga
     2101 ctgagcgttt agagaccatg gaaattcagt gctcatttgg aatactgcag gtattctagg
     2161 gcagaccgcc cattcctgcc tgagcataga taaatgcacc gggaccgatc tgtaagagcc
     2221 ctaatccaga gctttgtagc cttccggcag accgaaattg taaaggcggt cgaaactttc
     2281 tcgcacaccg tcttcgccgc gttttggttt ggaacctgag accagttcta ccaggcgctg
     2341 ccagctcaca tgcttgaaga tgctcaagtc ttcgctggct ttgcgatccc tttcgctttc
     2401 catgaccatg agcacgcggt cactgggcat gcgattgaag gtatctacgc agcgtccgtc
     2461 aaagtgattg cctttgcctg attccagaat ttcagccact ttctcaacgg gcatgcggtt
     2521 tctgtaatgg cgcaccgagg taagagcgtc aaagacgtcg gctacggcga tgatacgaga
     2581 gaggaaggga atttcttcgc ctttcagacc gcggtaatag cctgttccgt caagcttttc
     2641 gtgatggcaa ctggccacat agggcacggc ttgcagtcgc ttggtgaagg gcaggttgat
     2701 cagaagctcg taggtgattg aggcatgttt ttgcactttg gcgtattcat caggggtcaa
     2761 ccgaccgtcc ttccagagga tttcttccgg tacgcctatc ttgccgtagt cgtgcatcat
     2821 ggctgaatac ctgagcacat caagctcttg cgagaccacg tccatggccg tgcccaggat
     2881 gagactgtaa ttggtgaccc gcaccgaatg tccagccgtc aggggatcgc gtttatcgat
     2941 ggtctgggcc agcacttcca gggtcttatc gacgaagcgc tcgatttccg cataggcctg
     3001 ggcctgctcg atgagtccgg ccgccaccgc cgtcagcgct tccagccagt cttcgtcttc
     3061 ggaggtgaag ggccccgata acttattcaa aacctgaaat acaccgatga cagtgccttc
     3121 ccggttgcgc atcggtacgc agagaacgct tctggtctta tagccggttt gtttgtctac
     3181 tgccggatcg aagcgctcgt cctggtaggc gtcgggaatg ttgatgatgc gtccggagcg
     3241 ggcgcagtgg ctgacaatgg ctgttcccga gaggggaatg cggatggtgc gtacgcccac
     3301 cagcccctgg gctacctggg tccagagttc tcctttgtcc tgatcaagca caaagactga
     3361 acagcgatcg catttgacga tggcctgggt ttctctggtg atggtctcca gaagagaatc
     3421 gagcttgcgc tcggtggcca tggccttcac tacccggtag agaccggaga gcggcctgag
     3481 acgatccagt tcttcctgta acctgagcgc tttgatctca cgctcgatca ggccgatctc
     3541 tcgtttgttc agttcttctt ccatctgctt caggtctaca acagccgaag cggccgtctt
     3601 ttcgcctgtg gaagccggcg ccgccggctc ctgaccgtgt gcatgcccgt cgtgggctcc
     3661 tttgaccata ttattcgctc cgccaagaca tatagaatct taccaaacgg tg
//
LOCUS       DAOQNZ010000210         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11759_length_3710_cov_3.580301, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000210 DAOQNZ010000000
VERSION     DAOQNZ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11759_length_3710_cov_3.580301"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1042)
                     /locus_tag="PKW73_05050"
     CDS             complement(242..1042)
                     /locus_tag="PKW73_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72680.1"
                     /translation="MNNSFVLTPPPSLYRSEPEQAVAPLNLPPQEHHQTEPAQGPQPQ
                     SASHIASALHSLIEYKKGYEKTPPLKRQEVAHTLPFGPVRTIFLGKHWQENQFAGKIY
                     RRNQFEIVGDSSTRLTVRLSRLALSEKTRRNLISVVEQGRFRPNNHILFADSIEKLSE
                     FFGSATIGRNQYTCPAGQDNSAQPKLFHFDAATLIWVKGQAAILVEGKFQNESGNPSR
                     YFVGAFIPYETERGTMVMELALTAATREAYLLHKAELKQVLATLVPAA"
     gene            complement(1107..1814)
                     /locus_tag="PKW73_05055"
     CDS             complement(1107..1814)
                     /locus_tag="PKW73_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNA72681.1"
                     /translation="MAEARAFNFNDVKAPEDNDFKAAGKLLSNEAFTPPVVKPEQARA
                     DDNKIKTAPEQGREKTDTAVLTNPDKDKAPAILPNPEKDKAAEKHLPDLKIVDKDGKP
                     VKHALDKGHFSLAPGQCVEGIGSHYGRGDNTYGRRTASGEIFRAGKATVALPNFNNQK
                     EKPFDVTVQDMRTGKMIEARVNDMGPHKRLNRGVDIESATFKRLLGDSGLAHVRVCRP
                     SDKYSRNSHTLPGIIEL"
     gene            complement(2109..2873)
                     /locus_tag="PKW73_05060"
     CDS             complement(2109..2873)
                     /locus_tag="PKW73_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72682.1"
                     /translation="MGHSFTGGGGGDGGNGHGHGGHGHGGNGHGGHGHQSMGGHPDQG
                     ALWNSALQGVGGKGKPLTRVPARTFLPIIVAIVAFLAMMIPFAINWDEVEAVRREEEL
                     KAQQGAGQGATPDGQSPQSAQQAYGQIPGQAQYGQMPGQQYGQGSEMQGQMPNQAMMA
                     PTAGQLATQPAMQSPAMQAPAAPMANYAYAPGQTYGQPAYAPGQYYGQQPYAAPYDAS
                     GQLSALSGHSNRLESSGHMSPHNHLAQPKFKVFVAR"
     gene            complement(2993..3679)
                     /locus_tag="PKW73_05065"
     CDS             complement(2993..3679)
                     /locus_tag="PKW73_05065"
                     /inference="COORDINATES: protein motif:HMM:NF006743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HNA72683.1"
                     /translation="MSETAGPKLKDQEQVLSYIDSLAEELLAKIADGKRYILGITGAP
                     AAGKSTVSSLLKERINHLFGAVVAEVAPMDGFHKTNAELMQLGLWELKGIPTTFDAEG
                     FLEKLQAIQDDTASVGWPTFDRSIEEPTPDGVSINASHKLVLVEGNYLLLDTEPWSQV
                     SQYLDEIWYVDGDEETILPRLLERHMAGGKSPEAARAKVECTDLPNALLILQTKSSCD
                     RIICLPLGPA"
BASE COUNT          677 a          919 c         1095 g         1019 t
ORIGIN      
        1 gcttgtggcc gtggtcatgg cggcgctgtc tccctgcgcc tgtgccggca gtgccggtgt
       61 gaagggtagc aggctccagg atagaagcag gagcgaggcc cagtgggaaa cctttgaggt
      121 cttgaccatg ctggttggaa ttgtttttgt cacttgttag ccgccagagt aagcatttgc
      181 cctgcctgtg accgcaaggg tcaaggctga ggcatgattt taaccctaat gacagggtgc
      241 ttcaagcggc cggcacgagg gtggccagca cctgcttgag ttctgctttg tgcagcaaat
      301 aggcttctcg ggtggcggcc gtcagtgcca gttccataac catagtgccg cgctcggttt
      361 cgtaaggtat aaaggcgccg acaaagtagc gcgaggggtt gccgctttca ttttgaaatt
      421 tgccttccac cagtatggcg gcctgcccct tcacccagat taaggtagca gcatcgaagt
      481 gaaagagttt gggctgggcg gagttgtctt ggccggccgg gcaggtgtat tgattgcggc
      541 cgatggtggc gctgccgaag aattccgaga gcttttcgat gctgtcggca aagagaatgt
      601 ggttgttggg gcgaaatcta ccctgttcca ccacgcttat gagattacgg cgggtttttt
      661 ccgagagggc caggcggctc agtctgactg tgaggcgggt gctgctgtcg ccaactattt
      721 caaattgatt gcgtctgtag attttgccgg cgaactggtt ttcttgccag tgtttgccca
      781 ggaatatggt tctcaccggt ccaaaaggga gagtgtgtgc tacttcctgc cgcttgagcg
      841 ggggagtctt ttcgtagccc tttttgtact caatgagaga gtgcagggct gaggctatgt
      901 gcgaagcgga ttgaggttga gggccctgtg ccggctcggt ctgatgatgc tcctgcggtg
      961 gcaggttgag cggtgcaaca gcttgttcgg gctcgctgcg ataaagagag gggggtggcg
     1021 tgagtacaaa tgagttgttc attctagact ctctgcgttt gcttgaattt tgtagtaagg
     1081 aaggggtagt tcagccttga atttgcttag agttctatga tgcctggtag ggtgtgagaa
     1141 ttccgtgaat atttgtcgct tggtcgacaa accctcacat gggccagtcc gctgtcgccc
     1201 aggaggcgtt tgaaggtggc gctctctatg tcgactcccc ggttgaggcg tttgtgaggg
     1261 cccatgtcgt tgacgcgggc ttcgatcatt ttgccggtgc gcatgtcttg cactgtgaca
     1321 tcgaagggct tttctttctg gttgttgaag tttggtaagg cgactgtggc tttgcctgct
     1381 ctgaaaatct cgccgctggc ggttcgacgt ccgtaggtgt tgtctcctcg tccgtagtgt
     1441 gagccgatgc cctccacaca ttgtccaggt gccagtgaga aatgtccttt gtccagcgcg
     1501 tgtttgacgg gcttgccgtc tttgtcgaca attttcaggt cgggtagatg tttttcagct
     1561 gctttgtctt tttcaggatt tggcaagatt gccggcgcct tgtctttatc cggattggtt
     1621 aagactgccg tgtccgtctt ctctcttccc tgctccggtg ccgtcttgat tttgttatcg
     1681 tctgccctgg cctgttccgg cttgaccacc ggtggggtaa aggcttcgtt tgacaaaagt
     1741 tttccggccg ctttgaagtc attatcttca ggggctttta catcattgaa gttaaaggcg
     1801 cgagcttcag ccatgcactg tctccttcgg gggacaaagc gcaccaagaa aagtagatag
     1861 gtttttcgtc tggtgttaag gtggggtagg gtatacggct attgtgcata tggcttgtaa
     1921 gaaaattgtg aattgccttt cgcgcatccc ttcagttggt gcaaaaagaa aaccgatttg
     1981 cctggggcaa accggttttg ctaaagaaaa aagttatgga gcttggctgc aagcgatagt
     2041 ctcgcctcat ctttatgcca cagcgcagat aactttaaac tgttctatat ctgagtttct
     2101 gtttttatct atctggctac gaatacctta aatttcggct gcgccagatg attgtgcggc
     2161 gacatgtgac cgctggattc gagccggttg gagtgcccgg acagggcgct cagttggccg
     2221 gaggcgtcgt aaggcgctgc gtaaggttgt tgcccgtaat actgtcctgg agcatatgcc
     2281 ggttgtccgt aggtctgacc cggtgcgtag gcgtaattgg ccattggagc cgccggtgcc
     2341 tgcatagctg gtgattgcat agccggttgg gtggccagtt gaccggcagt gggtgccatc
     2401 atagcctggt tgggcatttg cccctgcatc tcactaccct gaccatattg ctgtccgggc
     2461 atctgaccat attgggcctg ccctggaatc tgtccgtaag cctgttgagc cgactggggg
     2521 ctttgtccgt ccggcgttgc gccctgtccg gcaccttgct gggccttcaa ttcttcctct
     2581 cgccgcacag cttccacttc gtcccagttt atggcgaagg gaatcatcat ggccaggaag
     2641 gctacgatgg ccacaatgat cggtaaaaat gttcttgccg gcactctggt caggggttta
     2701 cctttgccgc ccactccttg cagagcggaa ttccataacg cgccctggtc cgggtgtccg
     2761 cccatggact gatgcccgtg accgccatgg ccgttgccgc cgtgcccatg gccgccgtgt
     2821 ccgtgaccgt tgccgccatc gccgccgccg ccgcctgtga agctatgccc catatgtcgc
     2881 cccttgatgc tgccaagccg ttttcatttt atgtgcatgt ggtcactttt caaagtgaat
     2941 cagtggggaa atcaccagag cagcaatgaa aattgttggt aagtaacttg cttcaggctg
     3001 gaccgagcgg caggcaaatg atgcggtcgc agcttgattt tgtctgtaga attagcaggg
     3061 cgttgggaag atcggtgcat tccactttcg ctctggctgc ttccggcgat ttgccgccgg
     3121 ccatgtgtct ttccagaagc ctgggcaaaa tggtctcttc atcaccgtct acataccaga
     3181 tctcatctaa gtactggctc acctggctcc atggttctgt gtcaagcagc aggtaattgc
     3241 cttccacaag caccaattta tgactggcgt tgatgctgac accatccggc gtgggttctt
     3301 ctatggagcg gtcgaatgtc ggccaaccca cggaggcggt atcgtcctga atagcctgca
     3361 gtttttccaa aaagccttcg gcatcaaaag tggtgggaat tcctttcagc tcccagagcc
     3421 ctagttgcat cagttcggca ttggtcttgt gaaagccgtc catgggtgca acctcggcta
     3481 cgaccgcgcc gaacaaatgg ttgattcgtt ccttcagcag gcttgacaca gtgctctttc
     3541 ctgctgccgg ggcgccggtg atgccgagga tatagcgctt gccgtcggca atctttgcca
     3601 gcaactcctc agccaggctg tctatgtagg agagaacttg ttcctggtct ttgagcttgg
     3661 gaccggctgt ttctgacatt gattctaaag acctgattgg aggaatttat
//
LOCUS       DAOQNZ010000211         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11797_length_3705_cov_3.402740, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000211 DAOQNZ010000000
VERSION     DAOQNZ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11797_length_3705_cov_3.402740"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..872)
                     /locus_tag="PKW73_05070"
     CDS             complement(54..872)
                     /locus_tag="PKW73_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNA72684.1"
                     /translation="MGCENFSDCQKSAGDDNQGKGLSAATILDLLQQRGEKTGQHLNN
                     AGRKGDEALQELDKAAQEAQKAVETGLKDAAEQLPPAVEQARRDLGTLGRSLGNILDQ
                     GINALTPTDTKSLVSRLTDKFDDIDTDHDGFISRKELGESKNNLALKLTMGGTLNAAE
                     KHFDNLQGLNRDSLIWDNNGISRKDLQVLDSARRNGTGLGEAFANAPTLDLKQHVPSA
                     GLGFLAATASKAGTPGKALMLGATAFVAAEAAIGTMSYKLFTAPKANAIMEDLK"
     gene            1011..1703
                     /locus_tag="PKW73_05075"
     CDS             1011..1703
                     /locus_tag="PKW73_05075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HNA72685.1"
                     /translation="MSQSKPPERRFEVFADFDGTITTRDTLDVIMKELADPSYFVLEE
                     QWVRGEIGSRACLEKQVPLLRGGWPAMEEMLSEMQIDPTFPDFSLWLKEMAVPLRVVS
                     DGIDKIILHMLERAGLMQNGLIEAVVSNCLHVQDDGRLSIEFPNSRAESGCWSGTCKC
                     FALGKSAVAPAESLCVVIGDGLSDRCWAKNADILFAKGRLLHYCRENQIPCHPFQDFR
                     DVRAGLEKLFDL"
     gene            complement(1732..>3705)
                     /locus_tag="PKW73_05080"
     CDS             complement(1732..>3705)
                     /locus_tag="PKW73_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72686.1"
                     /translation="HYERALAALSVPSRQTAMKALVLRQKLAIYLAAGKLDKAQQTVD
                     TLSREFPDDWQTFTSRARLLKAKKKDEEALNELDKAIKLDIKNVDLLELKAHYLSDMK
                     EPTAALAYINTALIILSKTRHDATLDRETYSRCFYLRGLYLSELGQEQAAIGSFTQAV
                     QKNPRNAAALVERGQAFMRLDQLQNAIGDYRSALKIDADNWSALSNLADCYKSLHNYK
                     EAVKYYDLALAANPRWREAYYARANCRFMLKDYKGALADYNLALKHEGRLQEIYINRG
                     GTLGAMGDYQGLLRNLETAKTLGKPTRLMLTNEAIALNHLNRRKEALEKFQAAIELEP
                     KRIQSYLDLATFYAGTKEELPKAFAAIGSAMALEPDNPAALKAKFKLELRSKNFREAL
                     KTVEKYLSLCPQDLPMQVEAIRAELTIGEKGKALSRTEALLAERPKWIDGWFELGNCY
                     FELGRTEEALQAFQKVVALDKTDQEAMRRVAQCQRQLGDYESALKWLEQARASSGDSD
                     DLALQEAITLLSSARLSESKTILNRLARKSPQWALPHLYLGQCLILQGKYDDALSALR
                     RCTSRDPELAAALSSSALAQAKAGQLEASKESIARAVALGANDSLYYAACALINHLTG
                     KEAQALSDLNKAESLNKRDWSVMEVKKTLEQTD"
BASE COUNT          718 a          932 c         1021 g         1034 t
ORIGIN      
        1 cccggcacaa cttggtgtcc tgcctgccgc catattttta tttgattggc tccctatttc
       61 agatcttcca tgatggcatt tgctttcggt gctgtaaaaa gtttgtagct catggtgccg
      121 attgctgctt ctgctgcaac aaaagctgtt gctccgagca ttagcgcctt gccgggagta
      181 cctgcttttg aggcggtcgc cgctaagaag cccagaccgg cactgggaac atgctgtttc
      241 aagtccaggg tgggagcatt ggcaaaggct tctcccaatc cggtaccgtt tcttctagct
      301 gagtctagaa cttgcagatc tttgcggctg ataccgttgt tgtcccagat caagctgtct
      361 ctattcagtc cctgcaagtt atcgaaatgt ttttccgcgg cattcagggt gccgcccata
      421 gtcagcttga gagccagatt gttcttactc tcccctagtt cctttctgct gatgaagccg
      481 tcgtggtcgg tgtcgatgtc atcgaatttg tcggtcaacc gactgaccag actcttggtg
      541 tcggtggggg tgagggcgtt tataccctgg tcgagaatgt ttcctaaact tcgtcccaat
      601 gtgcccagat ctcgtctggc ctgttcaaca gccgggggca gctgttcggc ggcatctttc
      661 agaccagttt ccactgcctt ttgagcttcc tgcgcagctt tgtccagctc ttgcagggct
      721 tcgtctcctt tgcgaccggc attattaagg tgttgcccgg tcttttctcc ccgttgctgg
      781 agtaggtcaa ggatggtggc tgctgaaagt ccttttccct ggttgtcatc tcctgcagat
      841 ttctggcaat ctgaaaaatt ttcacaaccc atgtggacac ctcaaaagaa actatggcat
      901 taagataggt gagcttttgg aggctggcaa ttggggtaat tacgcttccg gcaaagttag
      961 cgtaaactct aatctgatga ggtttctaag tgcatcggga tgacctgaaa atgtcacaaa
     1021 gtaagccgcc cgaaaggcga tttgaggtgt ttgccgattt cgacggcaca atcaccacca
     1081 gagacactct tgatgtaatt atgaaggagt tggctgatcc ttcttatttt gtacttgagg
     1141 aacagtgggt gcgcggagaa atcggctctc gcgcctgcct ggagaagcaa gttccattgc
     1201 tgcggggcgg ctggccggcc atggaagaga tgttgtccga aatgcaaata gatcctactt
     1261 tcccggattt cagtctgtgg ctcaaggaga tggctgtgcc actgagagtg gtgagcgacg
     1321 gcatcgacaa aattattttg cacatgctcg aaagagcggg cttaatgcag aatggattga
     1381 tagaagccgt ggtcagcaat tgcttacacg tgcaggatga cgggcggctc tccattgaat
     1441 ttcctaacag tcgcgccgag tccggttgct ggtctggtac ttgtaagtgt tttgccctgg
     1501 gtaagagcgc ggtagcgcct gccgaaagcc tctgtgtagt tattggtgat ggactttccg
     1561 atcgttgttg ggccaaaaac gccgatattc tttttgccaa ggggcggctg ttgcactatt
     1621 gtcgagaaaa tcaaattccc tgccatcctt ttcaagattt tagagatgtg cgtgccggtc
     1681 tggaaaagct gttcgacctt tgagtttgtg agagcacttg ccggagccgg ttcagtcagt
     1741 ttgctcgagg gtctttttca cttccatgac cgaccagtct cttttgttta gtgactcggc
     1801 tttattcaag tcggataagg cctgagcttc tttgccggtc aaatgattga tcagtgcgca
     1861 ggctgcataa tataagctgt cgttggctcc cagtgcgaca gctctggcga tgctctcctt
     1921 cgaggcctcc agttgtccgg ctttagcctg ggccagggcc gaactgctca gggccgctgc
     1981 cagttccgga tctcgacttg tgcagcggcg caaagcactt aaagcatcat cgtacttgcc
     2041 ctgtagaatc aggcattgac cgagatagag atggggcagg gcccattgcg gagattttcg
     2101 cgccaggcgg ttgagaatgg tcttactctc gctcaggcgg gcgctcgaca gcagggtgat
     2161 ggcttcctgc agggccagat cgtcgctgtc gccactgcta gcgcgtgcct gttccagcca
     2221 tttgagggca ctttcgtagt cccccagttg cctctggcac tgggccaccc gtcgcattgc
     2281 ctcctggtcg gttttatcca gcgccaccac tttctgaaag gcctggagag cctcttcggt
     2341 tcggcccagt tcaaaatagc aatttcccag ctcgaaccag ccatcgatcc actttggtct
     2401 ttcagccaga agggcttcag tacggctcag ggcttttcct ttttcgccga tggtcaattc
     2461 cgctctgatt gcttctacct gcatgggcag gtcctggggg cagaggctca gatatttctc
     2521 cactgtcttg agggcctctc gaaagttttt gctgcgcaat tccagcttga atttggcttt
     2581 cagcgcggcc ggattgtctg gctccagggc catggctgag ccgatggctg caaaggcttt
     2641 aggcagctct tcttttgtcc cggcgtaaaa ggtcgccagg tcaagataac tttgaatgcg
     2701 cttgggttca agctcaatgg ccgcctggaa tttttccagg gcctctttgc gcctgttcaa
     2761 gtggttgagc gcgatggcct cgttcgtaag catgagcctt gtgggcttgc cgagggtttt
     2821 tgcggtttcc agattgcgca aaagcccctg gtagtctccc atggctccca gggtgccgcc
     2881 tctattgatg tatatttcct gcaatctgcc ttcatgcttg agggcgagat tgtaatcagc
     2941 gagggcgcct ttgtagtctt tgagcatgaa acgacagttg gccctggcat agtaagcttc
     3001 acgccatctg ggattggcag ccagagcaag atcgtagtat ttaaccgctt ccttatagtt
     3061 gtgcagtgac ttatagcaat cggccagatt actgagagcc gaccagttgt cggcatcgat
     3121 cttcaaggcg ctacggtaat cgccgattgc gttttgtagc tgatccagcc gcataaaggc
     3181 ctgtccgcgc tctaccagtg ctgctgcatt gcgggggttt ttctgaacgg cctgggtgaa
     3241 acttcctatg gccgcttgtt cctgccccag ttcgctcagg tagagccccc gcagatagaa
     3301 acaacgggaa taggtctccc ggtcaagtgt ggcatcatgt ctagttttac tgaggattat
     3361 tagagctgtg ttgatataag cgagcgcggc tgtgggttcc ttcatgtcgc tcagataatg
     3421 agctttcagt tccagaaggt cgacgttttt gatgtcgagc ttgatggctt tgtccagctc
     3481 attaagagct tcttcgtctt tcttcttggc cttcaaaagg cgagctcgag aagtaaaagt
     3541 ctgccagtca tcaggaaact cacggctcaa agtgtcaact gtttgttgtg ccttgtccag
     3601 tttgccggcg gccaggtata tggccagctt ttgcctgagc accagggctt tcatggccgt
     3661 ttgccgggaa ggtacgctga gggcggcaag ggccctctca tagtg
//
LOCUS       DAOQNZ010000212         3698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11833_length_3698_cov_3.773538, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000212 DAOQNZ010000000
VERSION     DAOQNZ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11833_length_3698_cov_3.773538"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PKW73_05085"
     CDS             complement(<1..1117)
                     /locus_tag="PKW73_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72687.1"
                     /translation="MTALAVTVALNCAPASAQQVSPGLPGGAISPIRLVDAQRIAAQP
                     TLPSLVPTDVTPIGLGRERELLKPGPTFYLLQKLPSRMWLNGSVEVSQRGETNVFFTN
                     SNKKADYVFRVLPNLSVGYRILPRTSIYTNYFVLKDVFADHGRLSYPTTQSLAWGIRH
                     EIPIRTRTNLQFDLQAREIWQASHFRQFDFLPGVTLTHVLNPSTIAFGNIQLQLRGKN
                     YFCAPTREIDPFYTVGVLHRRGMWTFSAVDTFVCNFRNGNAIPSQSNLSMIAQLEIAR
                     QVTKKVPGLVTFVRAEPVFNWASRGLPGISGFDFRIFGGLRYSFNKAATNVAMDQLRK
                     QLKESSLPPAMAPSDSKTPTDETASPLKTMEGEPAEAP"
     gene            complement(1309..2148)
                     /locus_tag="PKW73_05090"
     CDS             complement(1309..2148)
                     /locus_tag="PKW73_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72688.1"
                     /translation="MFTLKSTLTLPVILLALLTPARAAETQSDFVGESDPDVINALKF
                     SGQKRSIKDSLRLLDQAMAKHPERLYLHSWKARLLAEIYESEAAIKEADIVLAREANN
                     VLALGVKARALANLGQPEASLALFQKALTLKPENSYLLSQRGRLLRGEKRYGEALKDF
                     DRLVKQDEGDSINRSERAYCLMGLGGRANMEKACRDLEIINQMTRLKNYNSISRLGRI
                     YTELGRFDKAEETLKAGIAKHPVIVQLQADLLKVYESSGQTEKARQQKIKLTRIEEEL
                     GLR"
     gene            complement(2179..3171)
                     /gene="ilvC"
                     /locus_tag="PKW73_05095"
     CDS             complement(2179..3171)
                     /gene="ilvC"
                     /locus_tag="PKW73_05095"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNA72689.1"
                     /translation="MQKTAQIYYDKDVQLSPLTTSTIAVVGYGNQGRAHALNLKESGL
                     KVIAAARPGGKAWQQAEADGFSPMPIDEAVRAADVIALLLPDEVMPSVFRNNIKGNLQ
                     ESKTFVFAHGFAVTNRTIELPATADVILVAPTGPGRQVRSLYTEGKGLPALIAVEMDN
                     SGKALAKALAYAKAIGSTRAGAILTTFKEETVTDLFCEQAVLCGGMPELIKASFTTLV
                     ERGYQPELAYISCLKEVKLIADLLFTQGIDGMRSAISNTAKYGSAVAGPAIINSDAKR
                     RLGSLLNDIEDGKFARGFVREAEEGMPATREFVVQEANSKLAKVGKSMKETLSF"
BASE COUNT          751 a          910 c         1078 g          959 t
ORIGIN      
        1 gcggcgcctc cgccggctca ccttccatgg ttttgagcgg agaagcggtt tcgtccgtgg
       61 gcgtcttgct gtcggacggt gccatcgccg gtggtagcga agactctttt agttgcttgc
      121 gcaattggtc catcgccaca ttggtggccg ccttattgaa ggaatagcgc agtccgccga
      181 agatacggaa atcgaagccg cttattccgg gcagaccacg actggcccag ttgaagaccg
      241 gctcggctcg cacaaaagta accagccccg gcactttctt tgtgacctgc ctggctattt
      301 ccaactgggc aatcatagag aggttgctct ggctgggaat agcgttgccg ttacggaagt
      361 tgcacacgaa ggtatccacg gccgagaaag tccacatgcc gcgtctatgg agaacgccca
      421 cggtgtaaaa gggatctatc tcgcgagtgg gcgcgcagaa gtagtttttg ccgcgcaact
      481 ggagttgtat attgccgaaa gcaattgtgc tcggattaag cacatgggtg agtgtcacac
      541 cgggaagaaa gtcgaactgg cgaaaatggc tggcttgcca gatttcacgg gcttgcaaat
      601 caaactgtaa attggtgcga gtgcgaattg gaatttcgtg cctgatgccc caggcaagag
      661 attgagtagt tgggtagctg aggcgtccat ggtcggcaaa gacgtctttc agtacgaagt
      721 aattggtgta gatactggta cgcggtaaga tgcggtagcc cacggaaaga ttgggcagta
      781 cacgaaagac gtagtcggct ttcttgttgg agttggtgaa gaatacgttt gtttctccgc
      841 gctggctcac ttccaccgag ccgtttaacc acatgcggct cggtaatttt tgcagtagat
      901 agaaggttgg tcccggcttg agaagttccc gctcgcgccc caggccgatc ggtgtgacat
      961 cggtcggtac gagactgggt agggttggtt gggcagcaat tctctgagcg tcgaccaatc
     1021 ttatgggcga gatggcgccg cccggcaaac cgggactgac ttgctgggca gaggcaggag
     1081 cacagtttag agccactgtc acagccagtg ctgtcaaagt ctttccggtg aaagagactg
     1141 ttgccgcagc gatttccgta aggagcgggg tcgaaatttt cctgctgaaa gaaatgcgcc
     1201 ctggttgggt ggtaatcaag tggatgcctc ggtaatgcct tcagtgctgc aagactgaaa
     1261 gatgccatat tatacgtcaa agaggcagct ttaccgaagc ctcggcgctt atcgtaagcc
     1321 tagctcttct tctattctgg ttaatttgat cttttgctgt ctggcctttt ccgtttgtcc
     1381 gctgctttcg tagaccttga gcaggtctgc ctgcaattgc acaataacag gatgtttggc
     1441 gatacctgct ttgagggtct cttccgcctt gtcgaagcga ccaagctcgg tgtagatgcg
     1501 tcccaggcgt gagatgctgt tgtagttctt taacctggtc atttgattga tgatttccag
     1561 gtctcgacaa gctttttcca tattggctct accccccaga cccatgaggc agtaagcccg
     1621 ttcgcttcta ttgatggagt cgccctcatc ttgcttgacg agccgatcga agtctttcaa
     1681 ggcttcgccg tagcgctttt cgccgcgcag gagccgaccc ctctggctga gcaaataaga
     1741 attttcgggc ttcagcgtga gtgctttttg gaagagggcc agactggctt cgggctggcc
     1801 caggttagcc agggctctgg ctttgacgcc aagggccagc acattgtttg cttcccgggc
     1861 aaggacgata tcggcttcct tgatggcggc ttcgctttca tatatttccg ccagaagacg
     1921 cgctttccaa ctgtgcagat agagcctttc cgggtgcttg gccatagcct ggtcaagtaa
     1981 acggagagaa tctttgatgc tgcgtttttg gcctgagaac ttgagggcat ttattacatc
     2041 cggatcgctc tcaccgacaa aatcggattg agtttccgca gcccttgccg gtgtcagcaa
     2101 ggcgagcaat atcaccggca gtgtcaaggt acttttgaga gtgaacaaca aggatggccg
     2161 cctcggcgaa cctttcgcct agaagctgag tgtttctttc atgcttttgc ccactttggc
     2221 cagctttgaa ttggcttcct gaacgacaaa ttctcttgtg gccggcatgc cttcctcggc
     2281 ttcacgcacg aaaccgcggg caaacttgcc gtcttctata tcgttcaaaa gactgcccag
     2341 tctccttttt gcgtcactgt tgatgatagc cgggccggct acggcggaac catatttggc
     2401 cgtattggaa atagcactgc gcatgccgtc gataccctga gtgaacagca ggtcggcgat
     2461 tagtttcact tcctttaaac aggagatgta ggccagctcc ggctggtagc cgcgctcaac
     2521 gagggtggtg aaactggctt tgatcagttc gggcatgccg ccgcaaagta ctgcttgctc
     2581 gcagaagaga tctgtaaccg tttcttcttt gaaggttgtc aagatggcgc ctgctctggt
     2641 gctgccgatg gctttggcgt atgccagagc tttagccagg gccttgccgg aattgtccat
     2701 ttcgacggcg ataagggccg gcagtccttt gccttctgta taaagggaac gtacctggcg
     2761 accggggccg gtaggagcta caaggatgac atcggcggtg gccggcagtt ctatggttct
     2821 gttggtgacg gcgaagccgt gagcgaagac aaaagttttg ctctcttgca aattgccctt
     2881 gatgttgttg cgaaagacgg agggcatgac ttcgtcgggc aggagcagcg cgatcacatc
     2941 ggcagcccgg acggcttcat cgataggcat ggggctgaag ccgtcggctt ccgcttgttg
     3001 ccaggccttg ccgcctggtc tggcagcggc gattactttc agaccgctct cttttagatt
     3061 gagggcatgg gctcggccct ggttgccgta gccgacgacg gcaatcgtcg aggttgtaag
     3121 ggggctcaac tgtacgtctt tgtcgtaata gatctgggcc gttttttgca tggctatttc
     3181 atttctgttg tggcttgaat ttgttgactg ttttgttcga gcgccaggag gcgccgctac
     3241 aggcgcgatt gtacagcaca tgcttgagca cctggttgta atcggcctca ggcccaggcg
     3301 gtggagttgt gacgatgcca gcggtagtgt cactaaatgt ttccggttgc cgcagtctca
     3361 cattaaatga cgaaaagcgg accgcatggc tacttgggga aagattaaat gcctgggctc
     3421 acggccttgc ttggaaattc aggttaaacc ttcctggatg gtcttgtctt cctgctcgga
     3481 tgtgactatg ctttacgagt cgtttgaaac tttgctaatt ttcgttcttt ctcgcactta
     3541 gtatgagtga acgaggcagg gtctcgggtt ttctagacga ggggtgccgg ttctgaattt
     3601 cctagataac actcttgctg tagattcgcc tgctgggcaa gacaaaggtc gtttagaaaa
     3661 tcgcaccgga gaactgggtt caggctccgc cgccatgc
//
LOCUS       DAOQNZ010000213         3698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_11834_length_3698_cov_3.303047, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000213 DAOQNZ010000000
VERSION     DAOQNZ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11834_length_3698_cov_3.303047"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..724)
                     /locus_tag="PKW73_05100"
     CDS             complement(170..724)
                     /locus_tag="PKW73_05100"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNA72690.1"
                     /translation="MPGNLIRVPLVRQSTDYTCGVAALQSLLGYFAIEEREDKLAAEL
                     RSNRKQGTAFERIVKAAQKRGLSAQVQVGMTIPELKRCIDSRKPVLCLVQAWAEARNG
                     QSVNYEERWQDGHYVVAVGYDDDCFYFMDPSTLGHFAYIPRGEFEKRWHDTDGSRKLE
                     HFGMVFSNVKNVPKYEPEIATPME"
     gene            complement(977..1708)
                     /locus_tag="PKW73_05105"
     CDS             complement(977..1708)
                     /locus_tag="PKW73_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72691.1"
                     /translation="MAFHACKALSQSSKFSAEYLLATMMGSSQKSSAQDKLKIAALQD
                     SIRNTCGKDDEEIVTALKLSLQQYGPEHQTTVDIFNCNVHHMKSFSKAKQMADMMIQS
                     CKSSYGENSLKTIKWESELARLYREAKQDDYGNELYKKCLAKLEHSGDQSNEALHEKC
                     SLNGLIGLHYAQKKDYALADHYLQPAAEAAENYSNVSFRVCIIEGYLETERVLGKTEK
                     VKWAEAELKDAMSKLPVLETFPGCK"
     gene            complement(2096..2464)
                     /locus_tag="PKW73_05110"
     CDS             complement(2096..2464)
                     /locus_tag="PKW73_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72692.1"
                     /translation="MKASLKAIFAMKKLHHLVFALIASCFMDLPAFGQTAVGEYNKAI
                     ARAGEAATRHDMPGMERALQEAIQCCGGNASYQTRAEFALGDLYRDWQRYGDAYKHYQ
                     NAYVKSPNLSVKQLAASLID"
     gene            2697..>3698
                     /locus_tag="PKW73_05115"
     CDS             2697..>3698
                     /locus_tag="PKW73_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72693.1"
                     /translation="MTETFHAQEMPRQAQASRINWLENGSHKDSETQPEKQSGQTLTR
                     PDQAHFENGLLNSAYLKTAENTARESTPGVVKFSDAIKNKDENPKDGNSYQLPKLILD
                     GSIITSDPRILSQASKEAQKAQAKELPPPAKTESPPQLSEKPQTAERNRPKEVRESPD
                     KPPKAIDTRDLKEYLHGNGNGNGDGRSLPPIAADFQRLGLATLAEQEKADKLKPQIYV
                     SQKAGDPRQPLGSKDNPFQTIQQAVDKAPPLSVINVGPGIYKEKVKVGRSDLTIQTDS
                     GQPAVIEPGKGAGGRGDAAFSIGSDINNVAIKNFEIRNFTGTEAGIKVDGKNISNVTL
                     "
BASE COUNT          995 a         1025 c          837 g          841 t
ORIGIN      
        1 ccagcacccg acccaggggc aagccggtat cgctggaaat ggccagcgac tggaccactt
       61 gctccgatga aactacctcc agctgcaccg ccagatcgcc gagaaggtaa gtattgctta
      121 tggcgaccat caagactccc gagcaggact aaaagaggtg tgtaatacct tattccatgg
      181 gtgtagcaat ttccggctca tatttcggaa cattttttac attactgaag accataccaa
      241 aatgttcaag cttgcgggag ccatcggtgt cgtgccagcg cttctcgaat tccccccggg
      301 gtatataggc aaaatgcccc agggtggagg ggtccataaa ataaaagcag tcatcgtcat
      361 agcccacggc tactacataa tgaccatcct gccagcgctc ttcataatta accgactgcc
      421 cgttcctggc ttcggcccag gcctgcacca ggcaaagcac cggcttcctt gaatcgatac
      481 agcgtttcag ttccgggatg gtcatgccca cctgcacttg agccgaaagc cccctcttct
      541 gagccgcctt aacgatgcgc tcaaacgcag taccctgctt gcggttagaa cgcaattcgg
      601 cagccagctt atcttcacgc tcttcaatgg caaaataacc aagcaacgac tgcaaagccg
      661 ccacaccgca ggtgtaatcg gtggactgcc tgaccagagg cacccttatt aaattcccgg
      721 gcaacgggcg cccgctacct gccgcctccc ccggcaaaag ccagaagagt aagcaaagca
      781 gacagggaag cagagctgga agcagaagaa agcatctcga aatcgaaagc cttcgactga
      841 cacgtgaggc aattttgctt tgctcattgc atgccgccat catggaaaaa gccctcgtgc
      901 caaaatgtcc acccctgaaa aaccgttttg ggtttagctt atcaactaca actattcgaa
      961 ttttgcaaca gcgccgctac ttgcacccgg ggaaggtttc taagactgga agtttcgaca
     1021 ttgcatcttt caattctgct tcagcccatt tgactttctc tgttttcccc aacactcttt
     1081 ctgtttccaa atatccctca atgatacaaa cccgaaagga gacattgctg tagttttcag
     1141 ccgcttcagc agccggttgt agatagtgat cggcaagagc gtaatccttc ttctgtgcat
     1201 aatgtaggcc gatcagtccg ttcaaagagc acttttcatg cagggcttca ttgctctgat
     1261 ctcctgaatg ctcaagcttt gccaggcact tcttgtacaa ctcattgccg taatcatctt
     1321 gttttgcttc cctgtagagt cttgccaact ctgactccca tttgattgtt ttaaggctgt
     1381 tttcgccata actcgactta caagactgaa tcatcatgtc agccatttgt tttgccttgg
     1441 aaaacgactt catatgatgt acattgcagt tgaatatatc aaccgtagtc tgatgctctg
     1501 ggccgtattg ctgcaggctc aatttcaaag ccgtaacaat ctcttcatcg tcctttccgc
     1561 aggtattcct tattgagtct tgcagagcgg caatcttaag cttgtcttgg gcagaactct
     1621 tttgggaact tcccatcatc gtcgccaata aatattcagc gctaaatttg ctggactgac
     1681 tgagagcttt gcaggcgtgg aaggccaaat cgtttgcttc cttggaagcg gctccctttc
     1741 gcctcttttc gtcttcaaac agttttagat agacaagagc agcctcctgc gagaaatagc
     1801 ccctatcgag acattcggca cactgtagta aatttgcggg tttaccgtcg gtatagtaat
     1861 tacgactatt gcaagtcgac gaaactactt caaaagcctg cctttgtctg ccgcagaaat
     1921 aatcatttag ggcgccgacc aatttagcct cagtagctat ttggctatct ctgcatccag
     1981 ccaatagcgc caagcatttc tgggaagctc cgtactgccc acaggcaaac tggcaacgtg
     2041 ccatatttaa agctgccagc tgcttcactg atagattacg cgtatgtcaa atcacctaat
     2101 ctatcagtga agctgccagc tgcttcactg atagattagg tgatttgaca tacgcgttct
     2161 gataatgctt gtaagcatct ccatagcgct gccaatcccg gtacaggtct cccagggcaa
     2221 attcagctcg ggtctgatag gaggcattac caccgcagca ctgaattgcc tcttgcaatg
     2281 ccctttccat accaggcatg tcatggcgag ttgccgcttc accggccctg gcaattgctt
     2341 tgttgtattc ccccacggcg gtttgtccga aagctggcaa gtccataaaa catgaagcga
     2401 ttaaggcaaa aactaagtga tggagctttt tcatagcaaa gatggccttc agggaagctt
     2461 tcattttacg ctctacatgg ccgtgttgca aagtcggagg tcaaggctga agtcagcagg
     2521 aaagagaata tatctgccct tgccgcctct tccgttgtcg ataaagttct tgcatccggc
     2581 taggtcaacg gccctgagtc gaatgatgaa ttaccagaac cccatcgcca atttcatgag
     2641 tttttcacat agcttcccaa taataaatgg aattccaagc gcacttacca aaatacatga
     2701 cagaaacctt tcacgctcag gaaatgccgc ggcaggctca ggcaagccga ataaactggc
     2761 ttgagaacgg cagccacaaa gattccgaaa cccaaccgga gaaacagtcc ggacaaactc
     2821 tcaccagacc tgaccaggcc cacttcgaaa acggactgct caattcagcc tatctgaaga
     2881 cagcggaaaa cactgccaga gagtcgacac cgggagtggt taaattttcc gacgccatca
     2941 aaaacaaaga tgaaaaccct aaggacggca atagctacca gttaccgaaa ctaattctgg
     3001 acggaagcat catcacctca gatccacgca ttctcagcca ggcgtcgaaa gaggctcaaa
     3061 aagcacaggc gaaagagcta ccgcccccgg cgaagacaga gtcgccgccc cagctcagcg
     3121 aaaagccgca aacggcagaa aggaaccgtc ccaaagaagt gcgtgaaagc cccgacaagc
     3181 cgcctaaagc aattgatact agagacttga aagaatactt acacggcaac ggaaatggaa
     3241 acggcgacgg cagatcgctg ccgcccatcg cggcagactt tcagcgcctg ggtctggcca
     3301 cccttgccga gcaggaaaag gcagacaagc tcaagcctca aatctatgtc agccagaaag
     3361 ccggcgaccc gcgccagccc ctgggctcaa aagataatcc cttccagacc atccaacaag
     3421 ccgtagacaa ggctccaccc ctgagcgtga tcaatgtcgg tccaggcatc tacaaagaga
     3481 aggtgaaagt aggtcgctcc gacctgacca tccagaccga cagtgggcag ccggcagtaa
     3541 tagaacccgg caaaggcgcc ggcggacgcg gagacgccgc cttttccatc ggctccgaca
     3601 tcaacaacgt agccatcaag aatttcgaaa ttcgcaattt taccggcact gaagccggta
     3661 tcaaagtgga cggcaaaaac ataagcaatg ttaccctg
//
LOCUS       DAOQNZ010000214         3660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12036_length_3660_cov_3.335090, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000214 DAOQNZ010000000
VERSION     DAOQNZ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12036_length_3660_cov_3.335090"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PKW73_05120"
     CDS             complement(<1..218)
                     /locus_tag="PKW73_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72694.1"
                     /translation="MGEGNEDFSEGMSRRRFVKMAGGLFLALSAGGGALEAKQQDKSL
                     KQGSQASRGGGAAATGRRKSGFELGDWRG"
     gene            380..1945
                     /locus_tag="PKW73_05125"
     CDS             380..1945
                     /locus_tag="PKW73_05125"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNA72695.1"
                     /translation="MDATVKEGNSAVETKRENLIDAIERIKKGGAVKYHQKLKEEGKL
                     FVRDRLRLLLDPGFEVEDARFANCRDMELPADGVVTGIGQIEGKTVCFMANDSTVKAG
                     SWGWRTVEKIIRIQETAAKLRVPLIYLVDSAGARITDQVEMFPGRRGAGKIFHNQVAL
                     SGYIPQICLLFGPSAAGGAYIPAFCDVIIMNEGKASMYLGSPRMAEMVIGEKVTLEEM
                     GGAKMHCSVSGVGDVLVKSEEEAIHLCKTYLRFMPTNCDHQVPNLASLEPKAFEKNME
                     QVIPEKENIPFDMHQVIERLIDEGSWFEMKKLFAAEIITGFGRIGGRAVGIVANQPRV
                     KGGVLFVDSADKAARFVWLCDAFNIPLLFLADVPGFMIGTKVERDGIIRAGAKMVSAV
                     SSATVPKISVVVRKAYGAGLYAMCGPAFDPDACLALPTAWIAVMGPEAAVNAVYYNKI
                     MELPEADRPAFVEKKRSEYREDVDLYKLAAELVVDDVVHPLALRQELINRYNVYATKE
                     QKGYKKKRSVLPV"
     gene            complement(2020..>3660)
                     /locus_tag="PKW73_05130"
     CDS             complement(2020..>3660)
                     /locus_tag="PKW73_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72696.1"
                     /translation="PTESGSAQDEKPILGGTFIFKAANSTDLRSKFLAHTNQQTVNWH
                     DLDKEAVTTLLSQMSTRYPLLFRAVERLKPISLYRVWTLKWSSADGRRDPYLVSGPDR
                     IIFSDSFFKLSDKRHSLIHELAHLLDGSGEQSYSETWIRRMAPAMFRTKLLVQFLSLE
                     SYIEFTQSIESETGLPSIYAINNFKELLAEEVASKLKGMNEEHFLIFSNHWFGGLIQP
                     TRAQRQRNAAFKESMILYKKNARREAIPFLMQAKTTSSSSLMANVYLAECYFNIGEPE
                     RCIEESNQALKKLSNLGLKEFDGLKLDVLTTKAKALLDSNQYVLAKSTLNEALLGEPA
                     SEYYLRSRAHCNFKLGLEAEGLLDLYRAKGLDNLLCNAIGCEGYDPELTIRLLDSAIE
                     KKKSFESLCIRAATFQRLAIKSKNKLKTAKLIERAIEDLKMALNQSSLNAAEIQAQCG
                     LLYLDRGSVNQAAMHLQQALELDQENIPAMIGEAKILELSGNTTESHRRYTNIRRKLE
                     EMESKNITPLSTRGLHTLSKAEGLSIVHFERKPDDLEK"
BASE COUNT          822 a          933 c          907 g          998 t
ORIGIN      
        1 ccccgccaat cgccgagttc aaacccgctc ttgcggcgcc cggttgcggc agcaccacca
       61 ccgcggctgg cctgacttcc ttgcttaaga ctcttgtctt gctgctttgc ctcaagggct
      121 ccgcctccgg cactgagggc caggaaaagc cctcctgcca ttttgacaaa acggcggcgg
      181 ctcatgcctt ctgaaaagtc ttcgttccct tcgcccattc caagtttttc ccgaccttcg
      241 gtgcgaggtc tgccctgggt aatcttgaga attttatctc cgacttccca tatattgaat
      301 caacctttag attgagccgc ttggcaacat ctgtggttac aatttgaaaa ccaagtagcg
      361 aactcaacag tgaggcaata tggacgcgac cgttaaggaa ggaaattccg ctgtggaaac
      421 aaaacgtgaa aacttgatcg atgccattga gcgcatcaag aagggcggtg ctgtcaagta
      481 tcaccagaag ctgaaggaag aaggcaagct tttcgtgcgt gataggttga ggcttttgct
      541 cgaccctggt tttgaagtgg aagacgctcg ctttgccaat tgccgtgata tggaattgcc
      601 tgctgacggc gtagtaaccg ggattggtca aattgaaggc aaaacggttt gcttcatggc
      661 caacgattcc acagtcaaag ccggttcctg gggctggcgc accgttgaga aaattatccg
      721 cattcaagaa acggcagcta aactgcgcgt tcctctcatt tacctggtgg actccgccgg
      781 tgcacgtatc accgaccagg tggaaatgtt ccccggtcgt cgcggcgccg gaaaaatctt
      841 ccacaatcaa gtggcccttt ccggctacat ccctcagatc tgcctgctct tcggaccttc
      901 ggcagccggt ggcgcctata taccggcttt ctgcgacgtg atcatcatga acgaaggcaa
      961 agcctccatg tatctgggct ctccccgcat ggccgaaatg gtcatcggtg agaaggtgac
     1021 gctggaagaa atgggtggtg ccaaaatgca ttgctccgtt agcggtgtcg gcgacgtgct
     1081 ggtcaagtcg gaagaagaag caattcatct gtgcaagacc tatttgcgct tcatgcccac
     1141 caactgcgac catcaggtgc cgaacctggc ctctcttgaa cctaaagcat tcgaaaagaa
     1201 catggagcag gtgattcccg agaaagagaa tattcctttt gatatgcatc aggtgatcga
     1261 gcgcctcatt gacgaaggct cctggttcga aatgaagaaa ctctttgccg ctgaaatcat
     1321 caccggtttc ggtcgcattg gcggccgcgc cgtcggtatc gtggccaacc agccccgagt
     1381 caaaggtgga gtgctctttg tcgactctgc cgataaggcc gctcgctttg tctggctttg
     1441 tgatgctttc aatattcccc tgctcttcct cgccgatgtg cctggcttta tgatcggtac
     1501 caaggtagaa agagacggca tcattcgagc cggtgccaaa atggtttcag ccgtttcttc
     1561 cgccactgtg cccaagatct cggtggtagt gcgcaaggct tacggcgctg gtctctacgc
     1621 catgtgcgga ccggctttcg accccgatgc ctgcctggca ctacccactg cctggattgc
     1681 tgtcatggga ccggaagctg ccgttaacgc cgtctactac aacaagatta tggaactgcc
     1741 ggaagcggat cgccctgctt ttgttgagaa gaaacgctcc gagtacagag aagacgttga
     1801 cctctacaaa ctggctgccg agctggttgt ggacgatgtg gtgcatcccc tggctctgcg
     1861 ccaggagctt atcaatcgct acaatgtcta cgccaccaaa gagcaaaagg gctataagaa
     1921 aaagcgcagc gtgctgccgg tttaagtctg gtttccgaag cgattagctc aggcttgaag
     1981 agtgaaagag gcaggtagcg atacttgcct ctttaccttt tacttttcaa gatcgtccgg
     2041 ttttctctca aaatgcacaa ttgaaagacc ctctgccttg gagagcgtat gtaacccgcg
     2101 ggtggaaaga ggtgtgatgt ttttgctctc catttcttcc aacttacgcc ttatgttcgt
     2161 gtatcttctg tggctctcag tagtgtttcc ggataattcc agaattttgg cttcccctat
     2221 catcgccggt atgttttcct ggtcaagttc cagtgcctgt tgcagatgca tcgccgcctg
     2281 gtttacgcta cctctatcca gatatagcaa tccacattgg gcctgaattt ccgcggcatt
     2341 gaggctgctt tgatttagag ccatctttaa gtcttctatg gccctctcga tgagtttcgc
     2401 tgttttcaat ttgtttttgc tctttatcgc aagtctttga aaggtcgcag ctcttataca
     2461 cagcgattcg aaggatttct tcttttcgat agccgaatca agcaatcgta ttgttagttc
     2521 aggatcgtag ccttcgcaac cgatggcatt gcatagaagg ttgtccaaac ccttggcccg
     2581 gtaaaggtcg agaagcccct ctgcctccag acccagtttg aagttacagt gcgctctgct
     2641 tctaaggtaa tattcacttg caggctctcc caggagcgcc tcgttcagag tcgattttgc
     2701 aagcacatat tgattcgaat ccagcaacgc cttggctttt gttgttagaa catctaattt
     2761 gagtccatca aattctttca gaccaaggtt tgaaagtttc ttcagtgcct ggttgctttc
     2821 ttctatgcat cgctccggtt caccgatgtt gaaatagcat tcggctaaat acacatttgc
     2881 catgagcgaa gaagatgaag tagtcttggc ctgcattaga aacgggatgg cttctctacg
     2941 ggcattcttc ttgtagagta tcatggactc tttaaaggct gcatttctct gcctttgagc
     3001 tctcgttggc tgaatcagcc ctccgaacca gtggtttgaa aaaatcagga aatgctcctc
     3061 attcattcct ttgagcttgc tggccacctc ttctgctaat aactctttga aattatttat
     3121 tgcataaatg cttggcagcc ctgtctcact ctcaatgctt tgagtgaatt ctatatagga
     3181 ctcaaggctc aagaattgaa ccaagagctt tgtcctgaac atggcaggtg ccattctcct
     3241 tatccatgtt tcagaatagg actgctcacc gcttccgtcg agcaaatgcg ctaattcatg
     3301 aatcagtgaa tgtcgcttat ctgagagttt gaagaaacta tctgagaaga ttatgcggtc
     3361 cggaccgctc acgagatagg gatctcttct gccgtcagcg cttgaccact ttaatgtcca
     3421 gactctgtag agggaaatgg gtttcagtct ctcaaccgcc ctgaataaca gcggatagcg
     3481 tgtgctcatc tgagagagga gggtagtaac ggcttcctta tccaggtcgt gccaatttac
     3541 ggtttgttgg ttagtgtgag cgaggaattt actccttagg tccgtggaat tggcggcttt
     3601 gaaaataaat gttccgccta gaattggctt ctcgtcttgt gccgaaccgg attccgtcgg
//
LOCUS       DAOQNZ010000215         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12046_length_3659_cov_4.151221, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000215 DAOQNZ010000000
VERSION     DAOQNZ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12046_length_3659_cov_4.151221"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2956)
                     /locus_tag="PKW73_05135"
     CDS             complement(<1..2956)
                     /locus_tag="PKW73_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNA72697.1"
                     /translation="MRERSVELVTGPYRAGKTMWLLRELVDYLLRSPLTCQPVFLIVP
                     SHRYKGLVEERVDGILKELGAPGLIGLTILPFYDLCHSLLRRAGVSFRLLGDGLRAAV
                     LQKALGKVQGEGRLNSLASISEFAGTHAQMLELIDELERAGLSPRDVLSTLSRSAASD
                     SRYIELAGVYQAYWAELEALDVYDERKLAYKAREVARAGSSSLAPGIFAIDGFDRFNP
                     LQLSVLSAVSELSERTLISFDYVDAASAEKYGEYAWKDKSIKGLREAFQGAQGKFSER
                     FQGQDSARGEGVERLLSRTLDRAMEMEEVARSVKECLHAGTDTSQIIVVARSLKDYLP
                     AIKAAFERAGLEYYLDEAVPVASLPVIKYLLRLLSLVTNDFSRLEVIRTLRSPFCNLV
                     WLDLTSALVERLDRLSLSGMVVAGRADWCFQLKGADRDLEPAQAAVARLIERLEMKES
                     GTLSYFVAVVEDIIEDLLILPSDEEYADPLMAWEEHQGLLEFRRILSDMVFEDELLTP
                     VYGESVLTYQEFLKRLERAFENSNFRRPRSGSRAVTICSADLVPNRRFEVVFLTGLNE
                     GEFPRRGERSGFLSRDEVRKWAGLGIDIENPRQHESFEISLFTSLLERATSRLYLSAP
                     VYELSGEELVPSFFLTEGEESKVKSMQFAVPRQKSLLAPVSPMDYTLGFLFASEASAQ
                     TSTALKLSEALQQALPFAYQEEGETCARNLAEPLTLVQSRTSPGAMGRPGLTNGDMQE
                     QVGLGLIGVDLPEFWSVSRLSDYGKCPFKFWVSHILKVSPELEPEAGLDARLLGEVYH
                     KCMELFYQGLKVRGLNLLSEAQVVDGFFDDCIADALVWLLQERQVKKTEFWDYEKKEV
                     YFRLRRFLTKEKARALKDGGEFQPVAFEKAFGFEGAQGEVSAPPLVVQALPRGGSEVS
                     TVRLRGRIDRIDRSHDGRIKVVDYKAGSKRITIDDARRGSDLQMPVYALAVANMEEGQ
                     GPVCEGLFLSISQ"
     gene            complement(3003..3076)
                     /locus_tag="PKW73_05140"
     tRNA            complement(3003..3076)
                     /locus_tag="PKW73_05140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3040..3042),aa:Arg,seq:ccg)
     gene            complement(3160..>3659)
                     /locus_tag="PKW73_05145"
     CDS             complement(3160..>3659)
                     /locus_tag="PKW73_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72698.1"
                     /translation="DGKYLAVLCQVTAMEQGRCQVDVLNAADGKVLATYSKRWPYGDL
                     RWASDSSRFGFFTVEGRLLILGLNKSGGAAAGSPFTLEELAYLPRGDAFAFSADGKRV
                     VLTSKDEVAVIVEVAGGQVVKRYLVDFPAAVKGIKLRPVAWSKDGALIAVGGEEYSVL
                     FWNVK"
BASE COUNT          921 a         1137 c          882 g          719 t
ORIGIN      
        1 cctgagaaat gctcaggaag agtccttcac aaaccggtcc ctgcccctcc tccatattgg
       61 ccaccgccag agcataaaca ggcatctgca aatcactgcc gcgtctggca tcatcaatgg
      121 tgatccgctt ggaaccggct ttgtaatcca caactttgat gcgcccatca tgagagcggt
      181 cgatacggtc aatacgtcct cgcaacctca ccgttgagac ttcacttccc cctcggggca
      241 gagcctgcac cactaagggc ggcgccgaga cttctccctg ggccccttca aaaccaaagg
      301 ccttttcaaa agccacaggc tgaaactccc cgccatcctt taaagcccgc gccttttcct
      361 tagttaagaa ccgacgtaag cggaagtaaa cttccttctt ttcgtagtcc cagaactcgg
      421 ttttcttgac ctgcctttct tgcagcagcc agacaagagc atctgcaata cagtcatcaa
      481 agaaaccgtc caccacctga gcttcagaaa gcagattgag ccctctaacc ttcaagccct
      541 ggtaaaaaag ctccatgcac ttgtgataga cctcaccgag aagacgagca tccagcccgg
      601 cttccggctc cagctccgga ctgactttca aaatatggga gacccaaaat ttaaaaggac
      661 acttaccata atcggagagc cggctgacgc tccagaactc aggcaaatcc acgccaatca
      721 gtcccagccc cacttgctcc tgcatgtcgc cattggtcag ccccggccgc cccatggcac
      781 cgggagaggt gcgcgactgc accagggtga ggggctcggc cagattgcgg gcacaggtct
      841 caccctcctc ctgatatgca aacggcaggg cctgctgcag agcttccgac aacttaagcg
      901 ctgtagaggt ttgagcagag gcctcactgg cgaagagaaa gcccagggta tagtccatgg
      961 gcgacactgg agcaagcaaa gacttctgcc tgggtacggc aaattgcatc gacttgactt
     1021 tgctctcttc cccctcggtc aggaagaagg atggcaccag ctcttcacca ctcagttcat
     1081 aaaccggtgc tgagaggtag agcctggaag tggctcgctc cagaagcgag gtaaagagag
     1141 aaatttcaaa tgattcatgc tgccggggat tctcgatatc gatgcccagc ccggcccatt
     1201 tgcggacttc gtcgcgactg agaaaaccgc tacgctcgcc acgccgggga aactcgcctt
     1261 cgttcagacc ggtcaaaaaa accacttcga agcgccgatt gggaaccaga tcggcactac
     1321 agatggtgac ggcccgggaa ccgctacggg gtctgcggaa attgctattt tcgaaagctc
     1381 tctccagccg cttgagaaac tcctgataag tcagaacact ctcaccataa acaggcgtca
     1441 atagctcatc ttcaaaaacc atatcggaca gaatgcggcg aaactctaaa agcccctgat
     1501 gttcctccca ggccatgagg ggatcggcat actcctcgtc ggaaggcaaa atcaacaggt
     1561 cttcgatgat atcttccacc acggccacaa agtaactgag ggtaccgctc tccttcatct
     1621 ccaggcgctc gatcaaacga gctactgcag cctgggccgg ctccagatca cggtcggcac
     1681 ctttcaactg aaagcaccaa tcagccctgc cggccaccac catgcccgag agagagagcc
     1741 ggtccaggcg ctccaccaaa gccgacgtca aatccaacca gaccaggttg caaaagggac
     1801 tgcgcaaggt gcgtatcact tccaggcgcg agaaatcatt ggtgacaaga gagagcaagc
     1861 gcagcaaata cttaatcacc ggtaagctgg ctaccggtac ggcttcatcc agatagtatt
     1921 ccaaaccggc ccgctcaaag gccgccttga tagccggcaa atagtctttc agtgaacgcg
     1981 ccaccacaat aatctgactg gtgtcggtgc cggcatgaag acattccttg accgagcgag
     2041 ccacctcttc catctccatg gcccggtcga gagtacgtga gagaagccgc tccacaccct
     2101 cgcccctggc agaatcctgc ccctgaaaac gttcggagaa ttttccttgc gccccttgaa
     2161 aggcttcccg caagcctttt atactcttat ccttccaggc atattcgcca tatttctcag
     2221 cacttgcagc atccacataa tcgaaactaa tgagggtgcg ctcggaaagc tcgctgacgg
     2281 cgctcaacac tgaaagctgc aaggggttga agcgatcgaa gccatcaatg gcgaaaatac
     2341 ccggtgcaag ggagctgctg ccggcgcgag ccacttcccg cgccttatac gccaatttcc
     2401 gttcatcgta gacatccagc gcttccagtt cggcccagta agcctgatag acaccagcca
     2461 actcgatata gcgagaatcg ctggcggcag acctggacaa agtagagagc acatcgcggg
     2521 gcgacaaacc ggcccgctcc agttcgtcga taagctccag catctgcgca tgggtaccgg
     2581 caaactcaga aatactggcc aggctgttca gccgtccctc tccttgcacc ttgccgagag
     2641 ccttttgcaa gacagccgcc ctcagaccat ctcccaggag gcggaagctc accccggccc
     2701 gcctgagaag gctgtggcag agatcgtaga aaggcaatat cgtaagccca atcagaccag
     2761 gcgcgcccag ctcttttaga attccgtcga cacgctcctc caccagcccc ttgtagcgat
     2821 gggaaggtac gattaagaag acaggttggc atgtcagggg agaacgcagc aaatagtcca
     2881 ccagctcccg cagaagccac atggtcttgc cggcccggta gggtcctgtg actaattcga
     2941 cgcttctttc gcgcacaaaa ctcgcctgaa atgcctgact tgaatttaaa ttgttgaaaa
     3001 accgtgcccg tcgagattcg aactcgagac cttcggcttc ggagaccgac gctctatcca
     3061 gctgagctac gggcacatgg ctaacgccgc agtctatcta taaaaataca tctatagaca
     3121 acggctctca ttatactgga atagttgggt catggcgccc tattttacat tccagaaaag
     3181 caccgaatat tcctcgccgc ctacggcaat cagcgctcca tccttcgacc aggctaccgg
     3241 tctgagctta atacccttaa cagcggcagg aaagtcaacc agatatctct tcacaacctg
     3301 cccgccggcc acctcgacaa ttacggctac ttcatctttg ctggtaagca ccaccctttt
     3361 gccgtcggcg ctaaaggcaa aggcgtcacc tctgggcaaa taggccagtt cctccagagt
     3421 aaatgggctc cccgcagcgg caccaccgct cttattgagt cctagaatta ggagccgtcc
     3481 ctccacggta aagaaaccaa atctggagct atccgaggcc cagcgcaggt ctccataggg
     3541 ccaacgctta gaataagtag ccagcacctt accgtcggca gcattgagaa catccacctg
     3601 gcagcggccc tgctccatgg ccgtcacctg acagagcacg gccaggtact taccatccg
//
LOCUS       DAOQNZ010000216         3645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12140_length_3645_cov_3.570195, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000216 DAOQNZ010000000
VERSION     DAOQNZ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12140_length_3645_cov_3.570195"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1464
                     /locus_tag="PKW73_05150"
     CDS             85..1464
                     /locus_tag="PKW73_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72699.1"
                     /translation="MGTPRRFSFRDQSATVYRKDSSVFRFISRDYEPTYKHLMDSGLY
                     TELVRRELLVEHEETTAPTFVSDLDNCARVIKPVRIPFVSYPYEWSFSQLQDAALLTL
                     KVQKLALQYGMTLKDANAWNVQFCQGKPVFIDTSSFEMYKPGRPWGAYGQFCRFFLMP
                     LVLMARREIRLNRLLLIYPDGIPLDLGVSLLPWTVWTDLSLAAHLLATAVSHGFYKTS
                     GKKSDSTVSISGSALPNIIEHLEDTVAALRLKDKNSVWSDYYKDNTYSDKACASKQKI
                     VEDVLSAGERKTVWDLGANTGVYSRIAARHARQVLSMDYDALCVERNYRALKSEAVTN
                     ILPLLMDCASPSPAIGWALSERLSLPDRGPCDMLLALALIHHLSVGNSIDFEQVSDYF
                     AAMAREVLVEFVPREDEQVVRLMKQRTNYCLSYTRENFERAFLRNFRLVACHQVEDSN
                     RTLYHFERL"
     gene            1464..3128
                     /locus_tag="PKW73_05155"
     CDS             1464..3128
                     /locus_tag="PKW73_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72700.1"
                     /translation="MRDEALLSDGSARVQGAATTGPCPGKKPGLAAGLALYPLYYAVL
                     PVLISFNVRAANSVPLLNLLLPLVVCLLLGLTVLVVSRVLLRGRQESALLAFSIINAF
                     YAITYLLIAFEFALTVFHVGLAAHLAQYALLAIWLGLLLIGLTAVLKPVMVLPVVPIL
                     AACGRFLFVLQILVLLYHEGTYQLLVRPAVQARQAQIRQQACSRLSTNPAITGSPDIY
                     YIILDEMAGASVLSRVFGYDNSSFRSQLAERGFVIAGSSRSNFPLTRLSISSSLNLSN
                     LEFLPDLAGRGSEDYSVTNDLLQNSVVSRILADRGYSYVHIGSEMPPTDYSPFAKVSS
                     HPDLDEFAIRFIESTSLGFLPDLSQYLADSRRRTRLASLRALSESGELQGQAPSPRFV
                     FCHLLSPHEPFIFNHDGSSRTALMPVRRDGLWDAAARRAYVEQVRFMEARIVTCIDDI
                     LRQSKERPIIILQGDHGTYAEDELAREDPREALFKERYDILNAYLVPEKIKPSIYSSI
                     SPVNSFRLILSQFGYNLPLLPDRCFYSTYGRPFKIRDVTQRVLGRP"
     gene            complement(3208..>3645)
                     /locus_tag="PKW73_05160"
     CDS             complement(3208..>3645)
                     /locus_tag="PKW73_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNA72701.1"
                     /translation="MFSAAVGSSTSGAFIESAAGIREGARTGLAAIVTGLLFLASFFF
                     IPLLEPLQHLKYAYGPALICVGLMMLNSVKEIDVEDATEAFPAFATIAMMIFTYSIAN
                     GLTAGLVLYALMKLLTGRAGELKGGSIILAFMCLVYFVFGLHH"
BASE COUNT          754 a          958 c          981 g          952 t
ORIGIN      
        1 cggggcagtt ttaacggtct tgatgcctct gcaaaggtgt ttgcgtagaa tttccgtgcg
       61 cgggcgccgc tccggtgatt cctgatgggt acgccgcgcc gcttttcctt ccgagatcaa
      121 agtgccacgg tttatcgcaa agactccagt gtttttcgtt tcatttcccg ggattacgag
      181 cccacctaca aacacttgat ggatagcggt ttgtacactg agctggtgcg cagggaactg
      241 ctggtggaac atgaggagac aacggcgcct accttcgttt cggatctcga taactgtgct
      301 cgtgtaatca aaccggtacg cattcccttt gtgtcttatc cctatgagtg gtccttcagc
      361 caactccagg atgcagcctt gcttactttg aaggtgcaga agttagcttt gcagtacggt
      421 atgactctca aggatgccaa tgcctggaac gtacagttct gccaggggaa accggttttc
      481 atcgatacct ccagctttga gatgtacaaa ccaggtcggc cctggggggc ttatggtcag
      541 ttctgccgct ttttcttgat gcctcttgtg ctcatggctc gccgcgaaat aagactgaac
      601 cgtcttttac taatttatcc ggacggcatc cccttggatc tgggtgtcag tcttctgccc
      661 tggaccgtct ggacggatct ctccctggcg gctcatttac tggcgacggc ggtgtctcat
      721 ggtttctaca agactagcgg caagaaaagc gatagcaccg taagcatttc cggttcggct
      781 ctgcccaaca tcattgagca cctggaagac actgtggccg ctctcaggct caaggacaag
      841 aattcggtct ggagcgatta ctacaaagac aacacttaca gtgataaggc ctgcgcttcc
      901 aagcagaaga ttgtggagga cgtgctcagt gccggcgagc ggaaaactgt ctgggatctg
      961 ggtgcaaata ccggggtcta cagccgtatt gccgctcgtc atgccagaca ggtgctctct
     1021 atggactacg acgccctgtg cgtggagcgt aactaccggg cgctcaagag tgaagccgtc
     1081 accaatatct tgcccttgct catggactgt gcttcgcctt ctccggctat cggctgggct
     1141 ttgagcgaaa gattgagcct gcccgatcgg ggaccctgcg atatgcttct ggcgctggct
     1201 ttgattcacc atctgtcggt gggaaacagc atcgattttg agcaggtctc ggattatttt
     1261 gcggccatgg ccagggaagt gctggtggaa ttcgtgccga gggaagacga gcaagtggtg
     1321 cggcttatga aacagcgcac taactattgc ctttcctaca caagggagaa ttttgaaagg
     1381 gccttcctgc gcaatttcag actggtggcg tgccaccagg tggaagactc aaatcgcaca
     1441 ctttatcact tcgagcgttt gtgatgagag acgaggctct acttagcgac ggaagcgcca
     1501 gagtgcaggg ggcggcgaca accggcccct gccccggcaa aaagccgggg cttgccgccg
     1561 gacttgccct ctaccctctt tactatgcgg tgctgccggt gctcatctct ttcaatgtgc
     1621 gagccgccaa ttccgttccc cttttaaatt tgcttttgcc tcttgtcgtc tgcctattgc
     1681 tcggtctcac tgttttagtc gtttcgcgtg tattgcttcg cggccgtcag gaaagcgcac
     1741 ttctggcttt tagcatcatc aatgctttct atgccatcac ctatttgttg atcgctttcg
     1801 agtttgcgct aacggttttt catgttggtc ttgccgctca cctggcgcag tatgccctgc
     1861 tcgcaatctg gttgggactg cttctcattg gtttgactgc cgttctgaag ccggtaatgg
     1921 tattgccagt agtgccgatt ctggcggcct gcgggcgttt tctttttgtc ttgcagatac
     1981 tggtgctgct ataccacgaa gggacttacc agttgcttgt gcgtccggct gttcaagctc
     2041 ggcaggcgca gataaggcag caagcctgca gccgcttaag cactaaccct gccatcacag
     2101 gcagtcccga tatctattac attattcttg atgagatggc cggtgcttcg gtgctttccc
     2161 gggtctttgg ctacgataat tcttcctttc gcagccaact ggctgaaaga ggttttgtaa
     2221 tagctggtag tagccgcagt aatttccccc tcacccgcct ctctatttct tccagtctca
     2281 atctgtccaa tctggaattt ttgccggacc tggccggtag gggctcggaa gactatagcg
     2341 ttaccaatga cttgcttcag aacagcgttg tttccagaat cttagccgat cgcggttaca
     2401 gctatgtgca cattggttcg gagatgcccc ccaccgacta ttctcccttt gcaaaggtaa
     2461 gctctcaccc tgatctggac gaatttgcca tcaggtttat tgaatctacc tctttgggct
     2521 ttctgcctga tctttctcag tatctggccg atagtcgcag gcgtaccagg ctggcttcgc
     2581 tgcgggccct ctctgagagc ggcgaacttc aggggcaagc gccgtcgcct cgtttcgtat
     2641 tttgtcattt gctttctccc catgagccct ttatcttcaa tcacgacggc agcagtcgaa
     2701 cagccttgat gcccgtgcgc cgggatggac tctgggatgc ggcggcgcgc cgggcttatg
     2761 tggagcaggt gcggtttatg gaagcgcgca ttgtgacctg catcgacgac attctcaggc
     2821 agtcgaaaga gagaccaatc attattttgc agggcgatca cggcacctat gccgaggatg
     2881 aattggcgag agaagatccc cgggaagctt tatttaagga gcgctacgac attcttaacg
     2941 cctacctggt acctgagaag attaagcctt ccatctacag cagtatcagt ccggtgaata
     3001 gctttcgcct tatattgagt cagtttggct acaatctgcc gctgttgccg gaccgctgct
     3061 tctattccac ctatgggcga ccctttaaga ttcgcgatgt gacgcaacgg gtgttggggc
     3121 gtccctgact ctgcagcaac gccatcattg gtttttgaaa ctgtctggtg actacagtgg
     3181 ggttgtttta caaacgcgca gacaaaatca atggtgcaag ccaaagacga aatagaccag
     3241 gcacatgaag gccagaatta ttgaaccgcc tttcaattcg ccggcccgac cagtgagaag
     3301 tttcatgaga gcatagagca ccagtccggc cgtcagcccg ttggcgatgc tataggtgaa
     3361 gatcatcatg gcgatggtgg caaaagctgg aaaggcttcc gtggcatcct ccacgtcaat
     3421 ttccttgacg gaattgagca tcattaagcc cacgcagata agcgccggtc cataggcata
     3481 cttcaagtgt tgcaggggtt ctaagagcgg aataaagaag aaggaagcca gaaaaagtaa
     3541 tccagtaaca atggctgcca gtccggttct agcgccttct cttataccgg ctgccgattc
     3601 gatgaaggcg ccgctggtgg aggaaccgac ggcggcggag aacat
//
LOCUS       DAOQNZ010000217         3639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12179_length_3639_cov_3.310547, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000217 DAOQNZ010000000
VERSION     DAOQNZ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12179_length_3639_cov_3.310547"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKW73_05165"
     CDS             <1..217
                     /locus_tag="PKW73_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72702.1"
                     /translation="VSAPGLFSVLSIVGSKIPLVEILEAGMLLMGFSAFAGRSLYCFV
                     QPIKCATNALAERLPELFDGWESISQN"
     gene            complement(506..1393)
                     /locus_tag="PKW73_05170"
     CDS             complement(506..1393)
                     /locus_tag="PKW73_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA72703.1"
                     /translation="MSGSSQLLIACIIAAGCTLPIFIPVCGRWLTARLCGMSTPILSF
                     GVGTTKWTRVLGKFWNTEFRLAPLLFVGYVSIPGLYYMPDKARENAPAAGTEKEAELR
                     LYPLWKKLLVTTSAVLSCLLCAWMFLFIAYSGYGAPGFKNTTGQGATIERSFQPLSVT
                     QSATLASRSGKDQISTVVRTFAEVFDLLQAENQKQKLEVPYGIAAFADHEGMQVNPNG
                     FDVLWVVAVMLVVGAFLNSLPLVGLDGGYFLLLIWQGLTGRALTSRVISVHAHISTLI
                     LVCLMLWKVYYAFNHPVIL"
     gene            complement(1403..1588)
                     /locus_tag="PKW73_05175"
     CDS             complement(1403..1588)
                     /locus_tag="PKW73_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72704.1"
                     /translation="MKFKVTAITMHDGEVRELAALGLDIETKNGEQTKTEFTVELTEA
                     QAAGLKAHPAVISVTAI"
     gene            complement(1650..1910)
                     /locus_tag="PKW73_05180"
     CDS             complement(1650..1910)
                     /locus_tag="PKW73_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72705.1"
                     /translation="MISPDHYGKSIACALLMVEQLKQQLTDAKIDFVPAATETASIEI
                     GCTEFDENKMRVKLEISLAVSPEKAEQLLNAAGFSQGFPDCY"
     gene            complement(2059..2547)
                     /locus_tag="PKW73_05185"
     CDS             complement(2059..2547)
                     /locus_tag="PKW73_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72706.1"
                     /translation="MNATKTDFRRELYNLAKAKRTIYASNSAWYHKLAPCDPGTDLYN
                     LALQVRNCQSLVDTEILPTFFEKEAVELLLSLPNLYWFWRIVESSIIKAGNGRHVIGV
                     DAVRGAIAEERNSAARAYDLKKDISALNQIETALWVAAGILVVQSSLLSAPALLRNPV
                     YL"
     gene            complement(3307..>3639)
                     /locus_tag="PKW73_05190"
     CDS             complement(3307..>3639)
                     /locus_tag="PKW73_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72707.1"
                     /translation="KEAYILRWVEELDGEGKKTVRYQFDDKGRLQDLELHKTAGRPRR
                     VDYRFENGVLVKRTNFNEAGTVTSTVEKPNETVSWSNKAAWWVIPVKPEAPADAINVD
                     AEVSHGPY"
BASE COUNT          774 a          900 c         1002 g          963 t
ORIGIN      
        1 ggtctcagcg cccggcctct tctctgtgtt aagcatcgtc gggtcgaaaa ttcctttggt
       61 tgaaatttta gaggcaggga tgcttctcat gggtttctcc gcgtttgctg gaaggagcct
      121 ttattgtttt gtgcagccaa taaagtgtgc aacaaatgcc cttgctgagc ggttgcctga
      181 acttttcgat ggttgggagt cgatatccca aaactagctt gtttctgtgt tagtgttgtg
      241 gcgatattga gcgggaatta cgtgcttttg catgtatttg agagaagtgc ggcgctgcta
      301 tttttctgga tggggttgct tcttgagcta tgaaagaata tagtaataac cgcgaaaaat
      361 gtgaattgcg gaacctcgag ccttcccaaa tgcggtgcaa gatgaggcat tgcgcctccc
      421 ctgcaccgtt tcttgtgcaa gccgctgctg ccgaagtcca tctcaacttt ctagataggc
      481 tcggcagttc cggatggtcg gcaggttaaa ggatgaccgg atgattgaag gcgtagtaaa
      541 ccttccaaag catcaggcag acgagaatca gggtagaaat gtgcgcatgt accgatatca
      601 cgcgggacgt taaggctctg cccgttaatc cctgccagat gagcaatagg aaatagccgc
      661 catccaaccc gaccagaggt aggctgttta gaaaggctcc gactacgagc atgaccgcaa
      721 ccacccataa gacatcgaag ccgttcggat tcacctgcat tccttcatga tctgcaaatg
      781 ccgctattcc gtatggaact tcaagttttt gcttctggtt ctcggcctgc agcaggtcga
      841 aaacttcggc aaaggttcgc accacagtac tgatctggtc tttgccggaa cggcttgcca
      901 gtgttgcaga ctgggtaaca ctcaggggtt ggaagctacg ttctatggtt gcgccctggc
      961 ctgtggtgtt cttgaagccg ggtgctccat agccggaata ggctatgaac aagaacatcc
     1021 aggcgcaaag caagcaagac agtacggcag acgtggtaac caggagtttc ttccagagcg
     1081 gatagaggcg aagttccgct tctttctccg ttcccgcagc cggtgcgttt tctctcgctt
     1141 tatcgggcat ataatataac cccggaatgg agacgtaccc gacgaagaga agcggcgcca
     1201 ggcggaattc agtgttccag aatttgccca gtaccctggt ccattttgtc gttcctacac
     1261 cgaaggacaa gatcggtgtg ctcatgccgc aaagtcttgc cgtcaaccag cggccgcaca
     1321 ccggaatgaa aatgggcaag gtacagccgg cggctattat gcaagctatt agcaactgtg
     1381 aactaccaga catgacggcc gctcagattg cagtgactga tatgacggca ggatgagctt
     1441 tcaatcccgc agcttgcgct tcggtcagct ctaccgtgaa ctccgtcttt gtttgctcac
     1501 cgttcttggt ctctatatcc agacccagtg cggccagttc tcttacttcg ccgtcgtgca
     1561 tggtgattgc tgtgacttta aatttcatat tgttctccgg gaatttgatt gtgttattga
     1621 ctggggtcct ggctgtttcc gtcgaatgct cagtagcagt caggaaaacc ctggctgaaa
     1681 ccggctgcgt ttagcagttg ctccgctttt tccggtgaga cggcaaggct tatttccagc
     1741 ttgacgcgca ttttgttctc gtcgaactcc gtgcagccga tttcaatgga agcggtttca
     1801 gtggctgccg gaacgaagtc gatttttgcg tcagtcagtt gttgttttaa ctgctcaacc
     1861 atcagaagcg cgcaggcaat gcttttgccg taatgatccg gtgaaatcat gacgacgctt
     1921 ttggagccgg tgtcagtttg tttaggtggt gtgaattaga atctggacat agtctttacc
     1981 tcgaaataaa cagttgattg ttagttcgcg atgaacaccc gccggtaatc atcgcggcag
     2041 agatagcttt tttccggctt agagatagac gggattgcgt agaagcgccg gtgcactcag
     2101 aagcgaggac tgcactacca ggatgccggc agccacccag agtgcggttt caatctggtt
     2161 caaggcggaa atgtctttct tgaggtcata ggctctggca gcactgttac gctcctcggc
     2221 tatggcgcct cgaaccgcat ccactccaat aacatgacgg ccgtttccgg ctttgatgat
     2281 ggagctttct acgatgcgcc agaaccagta gagattcggc aagctcagaa gcagttccac
     2341 tgcctctttc tcgaagaagg tgggcaggat ttctgtatcg accagggact ggcaatttct
     2401 cacttgcagg gccagattgt agaggtctgt tcctgggtcg cagggagcca atttgtgata
     2461 ccaggcggag ttggatgcgt agattgttct tttggcttta gccagattgt acaattcgcg
     2521 gcgaaagtct gttttggttg cgttcatggt gtactccaga aaaattcaaa gtagaagttt
     2581 gttggtgtga gccctgctgt tgattgcggt ttactgaggc ggcttacggt tgagaaggct
     2641 ccttaaaagg gcgttctgga ccagaacaga gaactgaaca aaaggcgttt tttgtatgtc
     2701 ttgccgtttg gctcttttct tagttggaag atgctccagt gaatagacaa aagtcagggt
     2761 gcttaaaccc taactttctg ttggaagctc tcctataggg tctgctcctg acttggaacc
     2821 aggcagcgcc tttctattgc gctgagagaa cgtgataaag agcggccttt cgggtgatat
     2881 ttgcggttgc atgacagaga ttttgacgcc tgcgcctgcg tagattccgc aggcttcaga
     2941 ctcgctagac agattacgcg ccgctcactg tctcgtggac tgccttgaga gcgtcctacc
     3001 cttgctcaca gattcgacac agaggattgg gcgtctgaac cacgggcgga gtttctggag
     3061 agtggttttc aggttgggac caaccattac agtgggactt tcactgcctc tccctctgat
     3121 ttaatactat atgccgaaat agaggcgctc gaaaaaaatc accgacgcgg cggccaggcc
     3181 taaaagcccg ttaccagtcc gcgtcggctt agctttgtcg gctcttgcga ttgccatgga
     3241 agctgccttc agaggggagc tacggcgtca attgccggcg ccgtagttcc ttgcatgcct
     3301 ctgtcgtcag tagggtccgt ggctcacttc cgcgtcgacg ttgatggcat cggccggcgc
     3361 ttccggtttg accggaatca cccaccatgc cgccttgttc gaccaggaga cggtctcgtt
     3421 cggcttttcc accgtgcttg tcaccgtgcc ggcctcgttg aaattggtgc gcttgaccag
     3481 cacgccgttt tcgaagcgat agtcgacgcg acggggacgg ccggccgtct tgtgcagttc
     3541 caggtcttgc aggcgacctt tgtcgtcgaa ctgatagcgg acggtcttct tgccttcgcc
     3601 gtccagttcc tccacccagc gaaggatgta ggcctcttt
//
LOCUS       DAOQNZ010000218         3628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12247_length_3628_cov_3.017632, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000218 DAOQNZ010000000
VERSION     DAOQNZ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12247_length_3628_cov_3.017632"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /gene="lptB"
                     /locus_tag="PKW73_05195"
     CDS             complement(<1..579)
                     /gene="lptB"
                     /locus_tag="PKW73_05195"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNA72708.1"
                     /translation="MTASLTPREKSSGVKTLVIENLQKSYGGRRVVDGVSFHVSRGEV
                     VGLLGRNGAGKTTSFDMVLGLVKPEKGRITLGDRNIAQLPIHERSRLGVGYLPQEASI
                     FRKLSVVDNIKIILELKNIPRREFNSRIQTLLEQFSLDHLGQTIAVLLSGGERRRLEI
                     ARCLASEPEFILLDEPFTGVDPISIADIQGLIR"
     gene            complement(576..2018)
                     /locus_tag="PKW73_05200"
     CDS             complement(576..2018)
                     /locus_tag="PKW73_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNA72709.1"
                     /translation="MAAKRIFPKTLSLFSLMLISGSMPLSIFGITFDTTLPAVAQAPA
                     LPGAGDPGSSGPIDIVANEQEFAGDHVIARGNVRVLFKDSVIVAPLATLYRDPLTGQP
                     QRAIFTGHPRLTQGVNKIDADTLTFEMATSKIVADGHAHSEVINQSNAQAAPATAKAK
                     TEKDKPVKVAQADPEAGEGESEADPDAAETAPKVATTGSGSAAASEPAPTKIITDADK
                     QVYDKATGQFEAYGKVRVRAGDINVTADALKMAYGVDTKPEAAIFTGHVSATQGTNNT
                     QADRMTYFLNTKRLQADGHVRSKVIQQGRGTSQPAPVQPTTQEPTSEPIVNGATGKGT
                     LMQAQPLVNGGSKVGFSFSEGESTAPIIIVSDSQDYSKETGRLTAFGNVKIFYQDTVG
                     IGPKVILVRNVEGQAERVYFVGRSQITQPGKRWIADKIVMTVADRKVLAEGNTRAIIL
                     PKKQAEPASNFQLAQSQKKQLSSSRVEMAQ"
     gene            complement(2074..2667)
                     /gene="lptC"
                     /locus_tag="PKW73_05205"
     CDS             complement(2074..2667)
                     /gene="lptC"
                     /locus_tag="PKW73_05205"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNA72710.1"
                     /translation="MTWLFRSEYFKLFLALGILGTLAGLMFYAQYQADKEEKEYQANR
                     AQNPTAERVSVDNYELKEVDDSNRLRWKLSAKSGIFEKDKKIHLTDICMQYFDGEKVK
                     MKVIAPTGLANETNRQVELTSSDKQRVVAEGEEGKSRLETSRLELTKKNGFKATGGVN
                     IVWSGVAKVTGDYATGVFGKKELEDLKIIGNTHAIIQ"
     gene            complement(2678..>3628)
                     /locus_tag="PKW73_05210"
     CDS             complement(2678..>3628)
                     /locus_tag="PKW73_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72711.1"
                     /translation="PETTGTVSAPEAAKPTGNAAEQATSQPAEAKAGQEASSQTARVL
                     LSPGQTLSVDAGFEDIVCTNDVNLARKQRESFPDSAEASFIYAVALSRTCAVEDALKE
                     VRRARRLAEKEGGPAYFDRMIESYEKMLSYYPDDNQVRYHLAWAYYMKAYVLAKYSQP
                     KQGSPAYQEWVKAAANPQVRKEESEEDKAGTSSQSKTAVLNDMGHIRRTVESASPQAI
                     PQIQTYYKMAMEKLDQVLAANPTDIWARIYRAHLAYEAGGPMDASLKEWLKARDQNPD
                     NPAPYFFLGEGFLKQGNLKECLLNVSKAVALRSDKAKGGQ"
BASE COUNT          694 a          936 c         1011 g          987 t
ORIGIN      
        1 tctaatcaag ccctgaatgt cggcgatgga aatggggtcg acgccggtga aaggttcgtc
       61 aagcaagatg aattccggct ccgaagccag gcaacgggca atttccagac gacggcgttc
      121 accgccggag agcagcacgg caatggtctg acccaggtga tcgaggctga attgttccaa
      181 aagggtttga atacgggagt tgaattcccg gcggggaatg ttcttcagct ccagaataat
      241 tttgatattg tccaccacgg acagttttcg gaagatggag gcctcctgcg gcaggtagcc
      301 cacgcccaac cggcttcttt cgtggatggg caattgggcg atattgcggt cgcccagggt
      361 gatccgtccc ttttcaggtt tgaccagacc gagcaccatg tcgaaggaag tagttttacc
      421 ggcgccgttt cggccaagca gaccgaccac ttcgccgcgg ctgacgtgga aactgacccc
      481 gtcaactact cttctgccgc cataggattt ttgtagattt tcgattacaa gtgtttttac
      541 gccggatgac ttttctctgg gcgttaacga tgccgtcatt gtgccatttc taccctggat
      601 gaacttaact gttttttctg gctctgggct agctgaaagt tggaagccgg ctcagcttgc
      661 ttcttaggca gaataatcgc ccttgtgttt ccttccgcaa gaaccttgcg atcggctacc
      721 gtcataacta ttttgtcggc aatccagcgc ttaccgggtt gggtaatctg gcttctgccc
      781 acgaagtaca ctctctcggc ctgaccttcc acgtttctga cgagaatgac tttgggaccg
      841 ataccgacgg tgtcttgata gaagatcttg acgttgccga aggccgtgag gcgaccggtt
      901 tctttgctgt agtcctgact gtcggatacg atgatgatgg gagcggtgga ctcgccttca
      961 ctgaagctga aaccgacttt ggagccgcca ttgaccagag gctgtgcctg catgagggtt
     1021 cccttaccgg tggctccgtt tacgattggt tcggaagtgg gttcctgggt ggtgggctgc
     1081 acgggcgccg gctgactggt gccccgtccc tgctgaatca ctttcgagcg aacatgaccg
     1141 tcggcctgca ggcgtttggt gttgaggaag taggtcatgc gatcggcctg ggtattattg
     1201 gtaccctggg tagctgagac atggccggtg aaaattgctg cttccggttt ggtatcgaca
     1261 ccataggcca ttttgagggc atcggctgtt acgttgatat ctcccgctct cacccgcact
     1321 ttaccgtagg cttcgaattg accggtggcc ttgtcgtaga cctgcttatc ggcatcggtg
     1381 atgattttgg tgggcgccgg ttcggaggcg gcggcgctgc cactaccggt agtggccact
     1441 ttcggggctg tctcggcggc atccgggtcg gcttcgcttt cgccttcacc tgcttcagga
     1501 tcggcctgcg ctaccttgac gggcttgtct ttctcggtct tggctttggc tgtagccggt
     1561 gctgcctggg catttgactg gttgattact tcagagtggg cgtgaccgtc agccacaatt
     1621 ttggaagtgg ccatttcaaa agtgagggta tcggcatcaa ttttgttgac gccctgggtc
     1681 agacgcgggt gacccgtgaa gatggctctt tgcggttgtc cggtgagagg atctcggtag
     1741 agagtagcta agggagcaac gatgacgctg tctttgaaga gcactcgtac attgccccgg
     1801 gcgatcacat gatcgcctgc gaactcttgt tcgttggcga caatatcaat gggacctgat
     1861 gagccggggt cgccagcacc gggcagagcc ggtgcttgtg ccactgctgg tagtgtggtg
     1921 tcgaatgtga tgccgaatat ggacaacggc atactgccgc tgatcagcat cagggaaaac
     1981 agggataggg ttttcggaaa tattcttttg gctgccatag tttctgtccg gtttttgtgg
     2041 ctctctgaga aaatgtacct tagtatgtaa tgcttactga atgatggcgt gggtattgcc
     2101 gattatcttc aagtcttcca gttctttttt gccgaaaacg cccgtggcat agtctccggt
     2161 caccttggct accccggacc agacgatgtt aacacccccg gttgccttaa acccattttt
     2221 tttcgttaat tccaggcggc ttgtttccaa tcttgacttc ccttcctcgc cttctgctac
     2281 gactctctgt ttgtctgaag aggtaagttc tacctggcgg ttagtttcat tggccagacc
     2341 ggtgggggca atcaccttca tcttcacctt ttcgccatcg aaatattgca tgcatatatc
     2401 ggtcaggtga attttcttat ccttttcaaa gatgcctgac ttggcggaaa gcttccagcg
     2461 caaacgattg gagtcgtcga cttctttcag ctcgtagttg tcaacagata ctctctctgc
     2521 cgtgggattc tgggcccggt tagcctggta ctccttctct tccttatcag cctggtattg
     2581 ggcataaaac ataagcccgg ccagtgtgcc aagaattccc agagccagga aaagtttgaa
     2641 gtattcgctg cggaatagcc acgtcatagt actggaacta ctgtcctccc tttgctttgt
     2701 cgctgcgcag ggcaacggct tttgagacat tcaaaagaca ctctttcaaa ttcccctgct
     2761 ttaaaaaacc ctctcccagg aaaaaatagg gagccggatt atccggattt tgatctctgg
     2821 ctttaagcca ttcctttaaa gatgcgtcca tgggaccgcc ggcttcatag gctagatgtg
     2881 ctctgtatat acgagcccat atatcagtgg gatttgctgc cagtacctgg tcgagctttt
     2941 ccatggccat tttgtagtag gtctgaatct gcggtatcgc ctgcggtgag gccgattcaa
     3001 cagtgcggcg aatgtgcccc atatcgttca agacggctgt cttagattgg ctgctcgtgc
     3061 cggctttatc ttcttcgctt tcttccttcc tgacctgcgg attggccgcg gccttgaccc
     3121 actcttgata ggcggggctg ccttgcttgg gttgggaata tttggccagt acataggctt
     3181 tcatgtaata agcccaggcc aggtgatagc gcacctgatt gtcatcgggg taatagctga
     3241 gcatcttttc gtaagactcg atcatcctat cgaaataagc aggaccgcct tccttttccg
     3301 ccaggcgacg cgcgcgccgc acttccttga gggcatcttc cacggcacag gtgcggctca
     3361 gggccacggc atagatgaag cttgcttcag cactatcagg aaatgattcg cgctgctttc
     3421 tggccaggtt tacatcattt gtacaaacta tatcttcgaa gccggcgtca acagaaagcg
     3481 tctgaccggg gctcaggagc accctggcgg tctggctgga agcctcttga ccggcttttg
     3541 cctcggccgg ctgagaggtt gcctgctctg ccgcgttgcc ggtgggcttt gccgcttccg
     3601 gggcagagac tgtgccggta gtctccgg
//
LOCUS       DAOQNZ010000219         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12320_length_3614_cov_3.752459, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000219 DAOQNZ010000000
VERSION     DAOQNZ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12320_length_3614_cov_3.752459"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PKW73_05215"
     CDS             complement(<1..1156)
                     /locus_tag="PKW73_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72712.1"
                     /translation="MKDPRPNVNLILVRTAVASAQAALTRFNWLPGGSVFLTPVFASA
                     GGKAKGLTGKRLAQAQIEGFKAKPGQTISNMLSGCENSGFNISFGLGEADAFTPSIWQ
                     NALIGAFKKARELETNEAVVVIPRSVVRRFKKQKTASLYDFGRVTASTIARVLYQADD
                     YKTAPAEPRRRIANISVAFDGLSVPELNEVEQGLTHGQILGKAVNLSRDTANLPPNYC
                     TPSYLVKLAETVAWGSDGTIEVEVFDADQCAEFGMNAFLAVARGSQEPPKFIVMTYTP
                     KVNPQTTEVLALVGKGITFDAGGLDLKPADGMRDMKYDMCGGASVIASMQAIAAMNVP
                     VRVKAFIAATENMTGGAAYKPGDVYIGMDGKSYEIDNTDAEGRLTLVDAIS"
     gene            complement(1288..2160)
                     /locus_tag="PKW73_05220"
     CDS             complement(1288..2160)
                     /locus_tag="PKW73_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNA72713.1"
                     /translation="MTNLQGAKFALIATVFFTVLNALVKEIGQTLPLEETVFFRFLIA
                     YLVILPLTPKSAHLEFFKKDTPRHLHLFRAGLGVTAILCCFFGMTRLPLSEAMSLTYT
                     APLLVAILGQYFLRERVPPLRWLAIACGAAGVLIICRPKMGAELMPVAVMLAGSLARA
                     GSDILARALSSRASSQCLVVSFLGLGSIMIAPFAIANWQTPTSEQWLVLLLLGVSGGF
                     FQIFLTRAFQLSPASALAPFFYTSLLWAVLLGALIWNEVPGQNEILGSALIVLDGFLA
                     LWTLRTKVAKPLAG"
     gene            complement(2228..3058)
                     /locus_tag="PKW73_05225"
     CDS             complement(2228..3058)
                     /locus_tag="PKW73_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA72714.1"
                     /translation="MSETILTAVWAGAAIILAIGIFLYCRYWSKQGANLSSQSGYVTP
                     YVSVLSRLVLYLLSRLGAPYFFGRLYISGLQNMAFGNEKQRYIICPNHQFEHDVAVVS
                     YLIGARRWRFLADIEQVRGWRAPWMAWLGVIPVETENLRARVASMNSVVTALEREPGT
                     SFIIFPQGRLVKDNSLARKDFNTGPVIIAGRAAKATGDNFALLPMYIEYVTDPAKASL
                     GQRLLAFCGIKREFCGRTVYGCRVQIAKPIPVKADEERSKDEIVDELFQAMTALQNGT
                     "
     gene            complement(3082..>3614)
                     /locus_tag="PKW73_05230"
     CDS             complement(3082..>3614)
                     /locus_tag="PKW73_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA72715.1"
                     /translation="TSGEFIAGRGSEHQGEFWVKGKQRMDGYHGNQEATEKAIVDGWY
                     RTGDIGYVDEDGFLYITDRLARFAKVSGEMVPMGLVETLVRQLTGLDELSIHVTCIPD
                     ASKGERLVAFYTGLGSLAEAGVSGLIKQLSDQIDNLWIPKVCDFIQLSEMPIGATGKL
                     DMQALKATARDLTANS"
BASE COUNT          782 a          947 c         1028 g          857 t
ORIGIN      
        1 aggatattgc atctaccagg gtcagccggc cttccgcatc ggtgttgtcg atttcgtaag
       61 acttgccgtc cataccgatg tagacatcgc cgggcttgta ggctgcgccg ccggtcatgt
      121 tttccgtggc agctatgaag gcttttactc tcaccggcac attcatggcg gcaattgctt
      181 gcatggaagc gatgaccgag gcgccgccgc acatgtcgta tttcatatca cgcatgccgt
      241 cggccggctt aagatctaag ccgccggcat cgaaggtaat gcctttacct acgagggcaa
      301 gcacttccgt agtttgtggg ttcactttcg gtgtgtaagt catgacgata aacttgggcg
      361 gttcttgaga gccgcgtgcc acagccagga aggcattcat tccaaactca gcacactggt
      421 cggcgtcaaa aacttccact tcgatggtgc cgtcggagcc ccaggctacc gtttcagcca
      481 gtttgaccag ataagagggc gtgcagtaat taggtggaag gttggccgtg tctctggaaa
      541 gattgacggc tttaccaagt atctgaccat gggtaagtcc ttgttctact tcgtttagct
      601 caggcactga gagcccgtcg aaggctaccg atatgtttgc aatgcggcgg cgcggctcag
      661 ctggtgccgt tttgtagtca tctgcctggt aaagcacgcg tgcgatagtg gaggcggtta
      721 ctcttccgaa atcatataat gatgctgttt tttgcttctt gaagcggcgt acgactgagc
      781 gggggatcac cactaccgct tcattggtct ccaattctcg cgcctttttg aaggcaccaa
      841 tgagggcatt ttgccagatg gatggtgtaa aagcgtctgc ttcccccagt ccgaaagaga
      901 tgttgaaacc gctgttttca caaccggaga gcatgttgga tattgtctgg ccaggcttgg
      961 ccttgaagcc ttcaatctga gcctgagcca gtcgctttcc agtcagaccc ttggctttgc
     1021 ctccggcaga ggcaaagacc ggtgtaagga aaacgctacc gccgggtaac cagttgaacc
     1081 tggtcagggc ggcttgagcc gatgctacgg ctgttcttac tagaatcaga ttgacgttgg
     1141 gtcttgggtc tttcatgttg taactccaat attcgaattt aagatggtca ggcaaagtaa
     1201 caactgctgt cactttgtct gaccggtttt ctttcagtca ggtccaaatc gggtcgctga
     1261 aagatcaatt tgccgactgc tggttcttta gccggcaagc ggttttgcca ccttcgtccg
     1321 caaggtccag agcgccagga agccatccag cacaattaag gcgctgccca ggatttcatt
     1381 ttgtcccggc acttcattcc agatcagcgc accgagcaga acagcccaga gcaaagatgt
     1441 atagaagaag ggtgccagtg ccgaagctgg tgaaagctga aaggctctgg ttaagaagat
     1501 ctggaagaaa ccgccgctta cgccgaggag cagcagaact aaccattgtt ccgaggtggg
     1561 tgtttgccag ttggcaatgg caaagggcgc aatcattatt gagccgagcc ccaggaaaga
     1621 gaccaccagg cattgactgg aagctctgct actgagggcg cgcgccagta tatcactgcc
     1681 ggctcgggcc aggctgcctg ccagcatgac tgctacgggc ataagctctg ctcccatttt
     1741 gggccggcag ataatgagga caccggctgc accgcaggca attgccagcc agcgcagggg
     1801 aggtactcgt tccctgagaa aatactgacc gagaattgct accagtagtg gtgctgtgta
     1861 cgtcagtgac atggcttccg agaggggcaa ccgggtcatg ccgaagaaac agcagagaat
     1921 ggctgtcact cccagtccgg ctctgaaaag gtgcaggtgt ctgggggtat ccttcttgaa
     1981 aaattccagg tgggcagact ttggtgtcag cggtagtatc accaggtaag caatcaggaa
     2041 gcggaaaaac acggtttctt caagcggtaa ggtttgtccg atttccttca ccagggcatt
     2101 tagaacggtg aagaaaactg tagcgatgag agcgaatttc gctccttgca agttagtcat
     2161 acacgcctcc tccgctgagc ggaagtgaga tggacccgtc cctgggtctt gttgaaaagg
     2221 aatgtggtca ggtgccgttt tgcagggccg tcatagcctg gaagagttcg tcgacgattt
     2281 cgtctttgct tcgctcttca tcggccttga cagggatagg tttggctatt tgcacccggc
     2341 agccgtagac tgtgcgaccg caaaattctc ttttgatgcc gcagaaagcc aggagccttt
     2401 gccccaggga tgccttggcg gggtcagtga cgtactcgat gtacatgggc aaaagcgcaa
     2461 agttatcacc ggtggccttg gcggcacgac cggcgataat caccggtccg gtattgaagt
     2521 ctttacgtgc caggctgttg tctttcacca gtcttccctg tggaaagatg atgaagcttg
     2581 tgccgggctc cctttcgagg gccgttacca cggaattcat ggatgcgacg cgggcgcgca
     2641 gattttctgt ttccaccggg atgacgccca gccaggccat ccatggggcc cgccagccgc
     2701 gcacctgctc gatgtcagcc agaaagcgcc accgtcttgc cccaatcaga tagcttacga
     2761 cggcaacgtc gtgctcgaac tggtggtttg gacagatgat gtagcgctgt ttttcattgc
     2821 cgaaagccat gttttgcagg ccgcttatgt acaggcgacc aaagaaatat ggcgcaccca
     2881 gcctggacag caggtagaga acaagcctgg acagcaccga cacatacggg gtgacatagc
     2941 cgctctggct ggacaagttt gcgccctgct tgctccagta gcgacagtag aggaaaatgc
     3001 cgatagcaag aattattgcc gctcctgccc agacggcggt taaaatggtt tcgctcataa
     3061 tttgttccgc aggcttaagt ttcaggaatt ggccgtcagg tctctggctg ttgctttcag
     3121 ggcttgcata tccagtttgc cggtggcgcc gatgggcatt tccgacaact gaatgaagtc
     3181 gcagaccttg ggaatccaga ggttatctat ctgatcggac agttgcttga ttagcccgga
     3241 aacgccggct tccgccaggc ttcccagacc ggtatagaag gccaccagcc gctctccctt
     3301 gctcgcgtcg ggaatgcaag tgacgtgaat ggaaagttcg tccagtccag tgagctggcg
     3361 caccagggtt tccaccagac ccatgggcac catttcaccg cttactttgg cgaagcgggc
     3421 caggcggtcg gtgatgtaca ggaagccgtc ttcgtccaca tagccgatgt cgccggtgcg
     3481 ataccagcca tccacgatgg ctttctcggt cgcttcttga ttgccgtgat agccgtccat
     3541 gcgttgtttg cctttcaccc agaactctcc ctggtgctcg ctgccgcgcc cggctatgaa
     3601 ttcaccgcta gtgg
//
LOCUS       DAOQNZ010000220         3604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12369_length_3604_cov_3.609749, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000220 DAOQNZ010000000
VERSION     DAOQNZ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12369_length_3604_cov_3.609749"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..468
                     /locus_tag="PKW73_05235"
     CDS             70..468
                     /locus_tag="PKW73_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72716.1"
                     /translation="MNFDPASQDSSDSFIEESHGCSFCDSEEFLDLDIYSTSSQPTEI
                     EYTPPISLVEATVVDAIIMGSGGPENGPITDAGLRFVRHIISQSRASNAGFLPNLKDM
                     NCIKELARGYIAGCGSGTGALETMYTVMHL"
     gene            complement(479..1246)
                     /locus_tag="PKW73_05240"
     CDS             complement(479..1246)
                     /locus_tag="PKW73_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNA72717.1"
                     /translation="MGLLEGKRGIVFGVANDHSLAWYCAQQMHAQGAQLAFTYQAENL
                     ERRLRPLAESVGSTFIRQCDLSKDEEIEKVMGELGKEWDSLDFIVHAVAFANKADLEG
                     HFVDTSREGFRLALDVSAYTLTAVAQKAMPLMKNGGSIVTLTYYGAEKVVPHYNVMGV
                     AKAALEASVQYMAADLGEKGIRVNAISAGPVRTLAAMGIAGFREMLKVVEQKAPLKRN
                     VQAEEVGKTALYLASDLSSGVTGEVIHVDCGYSIMGM"
     gene            complement(1359..1940)
                     /locus_tag="PKW73_05245"
     CDS             complement(1359..1940)
                     /locus_tag="PKW73_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNA72718.1"
                     /translation="MKSQKIALAAVVAGSVLFLGLTSAILTFPGNEIRFSPEPAFAAG
                     GEGENGEKSKSDIQSDKAIKELKWYHSFAEALAEAKSQNKLVFADVYTDWCGWCKRLD
                     RDTFSDPSMVAYLSEKYICVKVNAENGKENIKLAEDAEVTGFPTGLVFKSGGELLGKV
                     SGYKDAFGYKAALESLEQGNSEPGGAASPDTSK"
     gene            complement(2185..>3604)
                     /locus_tag="PKW73_05250"
     CDS             complement(2185..>3604)
                     /locus_tag="PKW73_05250"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNA72719.1"
                     /translation="QEELRWLERDPDRYSSLIADSVFSLAKRNFAPASERLTSVCARL
                     EKAPALIDAARRNLSTRPQEVPKIYAEVAVEQLAGTVSMLKTTIPEAFASVKDEGLKA
                     RFSLAQKNVLASLAEYEGFLKEKVLPVASGSYAIGEKLYSRKLSLDEMEDEKLSLLLE
                     RGYKELERLQSRFVSLGRSIDASRPAGAVFEDIAREHPTADELVAATQGCLDRLAKFC
                     QEESIVTIPSEDRVTVAESPSYLRALTFASMDTPGPYENKAKEAFYYVTPAEKNWDPK
                     RIEEHLRFFSHADIINTSVHEAYPGHYVQFLWVKHAPSKVRKLLGCSSNAEGWAHYCE
                     EMMVQEGLVQPGTVGPDHKLAMVQVHDALLRVCRYIVGIEMHTRGLSYDKAVAFFMKE
                     GYMEKANAERETKRGTKDPTYLVYTLGKLKILAIRDEYKAKMGAKFSLKNFHDAFLKC
                     GFPPLKIVRAELLDLPVPEAGR"
BASE COUNT          899 a          968 c          845 g          892 t
ORIGIN      
        1 gaccctaaag tggggtcacg tctcaacaat atctttattt ttcatggcgt tttcagggga
       61 tattaatcga tgaactttga tccggcatct caagatagta gcgactcttt tattgaagaa
      121 tcgcacggtt gtagtttctg cgacagcgaa gaatttcttg acctggacat ttattcaact
      181 tcgagccagc caaccgagat tgaatacaca ccgcctattt ctctagttga agcgactgtt
      241 gtggatgcca tcatcatggg cagcggcgga ccggagaacg gtccaatcac cgacgccggg
      301 ctgcgctttg tcaggcacat catctctcaa tcaagagcaa gcaacgcagg cttcctgcct
      361 aacttgaagg acatgaactg catcaaagaa ttagcgcggg gctacattgc cggctgcgga
      421 agcggaaccg gcgccctgga aaccatgtat acagtgatgc atctgtagag ggaactctct
      481 acatacccat aatgctatat ccgcagtcaa cgtgaatgac ttcaccggta acccctgaag
      541 acaggtcact ggccaggtaa agagctgtct taccgacctc ttccgcctgc acattacgct
      601 tgagaggcgc cttctgttca accactttga gcatctccct gaagccggcg atgcccatcg
      661 ctgcgagggt gcgaaccggt ccggcagata ttgcgttgac gcgaatacct ttctcgccga
      721 ggtcggcagc catgtattgc acgctggctt ccaatgcggc tttggcaacg cccatcacgt
      781 tgtagtgggg aaccaccttt tcagcgccgt aataagtaag agtgacgatg gagccgccat
      841 ttttcatcaa cggcatggct ttctgggcaa ctgccgtaag agtgtaagca cttacatcca
      901 gagcgaggcg aaagccttcc cggctggtgt ccacaaagtg accttccagg tctgctttgt
      961 tggcaaaggc tacggcgtgc acgataaagt ccaggctgtc ccactctttg ccgagttcgc
     1021 ccatgacttt ctctatctct tcgtctttgg aaagatcaca ttgtctgatg aaagtagaac
     1081 ctaccgactc agctagcgga cgcaagcgcc tttccagatt ttcagcttga taggtaaaag
     1141 ccaactgggc cccttgagcg tgcatctgtt gggcacagta ccaggccagg ctgtggtcgt
     1201 tggccacgcc aaatacaatt cctcttttac cttcgagcaa acccatcttt tttctgtcct
     1261 tattaatact ggtaggtcaa ataaaacaaa gtgaccttga ttatggtaca ggaaaaagac
     1321 agcaaagctt gatgagctta agcaaagctc aggtttgtct atttggaagt atcaggagaa
     1381 gctgcgccgc caggctccga attgccctgc tctagactct ccaatgccgc tttgtagcca
     1441 aaagcatctt tgtatccgga gacttttcca agcaactcgc caccactctt gaaaaccaac
     1501 ccggtgggga aacccgttac ctctgcgtct tcagccagct tgatattctc tttgccattt
     1561 tcagcattta cctttacaca aatatacttt tcagaaagat aagcaaccat ggacggatcg
     1621 ctgaaagtgt ctctatcaag acgcttgcac cagccacacc aatctgtata aacatcggcg
     1681 aaaaccagtt tgttctgcga cttggcttcg gcaagggcct cggcaaaact gtgataccac
     1741 ttgagttcct taatagcctt atcgctctgg atatcggact tactcttctc gccgttctcg
     1801 ccctcccctc cggcagcaaa ggccggctcc ggcgaaaatc gaatttcatt tcccgggaaa
     1861 gtcaaaatag cggacgtcag acccaggaac aggacagaac ctgcaactac tgcagccaga
     1921 gcaatttttt gcgacttcat gaaaacatcc ccataaaaat gagctccaga ttgaattacc
     1981 ttcttgaccc tgaatcaggg aaatttaggg acaacgaaag catttaggcc cggctctcag
     2041 aatctaaaga cggggcagtt cccggaaagt taccgtttct aatgacaatc tcgaaattct
     2101 taacatatca agtgtgaact ccgaacctgg cttatttcgt tcaagtcagg tcatgggaaa
     2161 gttcaagcga tatcgcttta gcttttaacg accagcctca ggcaccggta aatcaaggag
     2221 ttcagcccgc acgattttta agggaggaaa gccacacttg agaaaagcat cgtggaagtt
     2281 tttgagagaa aactttgctc ccatttttgc cttgtactca tcccttatgg caagaatttt
     2341 caacttgccc agggtgtaca ccaggtaagt agggtccttg gttcctcgtt ttgtttcacg
     2401 ttcggcgttg gctttctcca tatagccctc cttcatgaaa aatgccactg ctttgtcata
     2461 agaaagcccg cgcgtatgca tttcaatgcc gacgatataa cgacagactc tcagaagcgc
     2521 gtcgtgaacc tggaccatag ccagcttatg gtctgggcca acggtgccgg gctggacgag
     2581 cccttcctgc accatcattt cttcgcaata gtgagcccaa ccttccgcat tggaactgca
     2641 ccccagaagt tttcgcacct tagaaggggc gtgcttgacc cagagaaatt gcacgtagtg
     2701 accgggatat gcctcatgca cactggtatt aatgatgtcc gcgtggctga agaaacgcag
     2761 atgctcttca attctttttg ggtcccagtt cttctcggca ggcgtcacgt agtagaaggc
     2821 ctcctttgct ttattttcgt aggggccagg cgtatccatg gaggcaaatg taagggctcg
     2881 cagataagaa gggctctcgg ctacggtcac acggtcttca gagggaatag tgacaatact
     2941 ttcctcctgg caaaacttcg ccagtcgatc cagacaaccc tgcgtggcag ccaccagttc
     3001 atcggcagtg ggatgctccc tggcaatgtc ctcaaaaacg gctccggcag gacggctggc
     3061 atcaatgctc cggcccagag agacaaaccg ggactgcaag cgctccagtt ccttgtagcc
     3121 gcgctccaaa agcagcgaca gtttctcatc ctccatttca tcgagactta atttgcgact
     3181 gtagagcttc tcaccaatgg cataagaacc actggccacc ggcaatactt tctctttcag
     3241 gaaaccctca tattcagcca gtgaagcaag tacattcttc tgggccaggc tgaaacgcgc
     3301 cttcaaccct tcatccttaa ctgaggcaaa ggcttccgga atagtagttt tcagcattga
     3361 aacggtgccg gcaagttgct caaccgccac ctcggcgtag atctttggca cttcttgcgg
     3421 tctcgttgat aggttccgtc tggccgcatc aattaaagca ggcgcctttt caagtctggc
     3481 gcacacgctg gtgaggcgct ccgaagccgg cgcaaagttt cttttagcca gagaaaaaac
     3541 agaatcggca atcaaagagc tgtatcgatc cggatctcgc tcaagccagc gcaattcctc
     3601 ttga
//
LOCUS       DAOQNZ010000221         3597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12402_length_3597_cov_3.709204, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000221 DAOQNZ010000000
VERSION     DAOQNZ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12402_length_3597_cov_3.709204"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PKW73_05255"
     CDS             <1..1037
                     /locus_tag="PKW73_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNA72720.1"
                     /translation="MVGAGMAQIGEFSFILLSMGRDLKMINDYMYNLFFAGAVVSLIA
                     SPALFAASPKLSHWLRFLGAFFKKNQNSEEEEGHDASRLAGRLKDHVIICGFGRVGRN
                     LGTVLSSYGIPYLVIELNAGIIDDLAGRGIPHLYGDATSNMIMIKANLKEAACLVLTM
                     PDPSAITAITRFVRSRNETISIIARAHRQADIAAFTACGVSAIVQPEFEASIEITRLA
                     LFSRRRAPEEIEAALEKVRAMRYPHPSPDFEAMDRAPLYMMLEEEQMGRWFLNVDDNM
                     TGKSPKDLNVRGLTGVTITAIKRQEKTHTYPSPDFPFSPGDQIYAVGKKEELVRFQEH
                     FKMRQFSPVP"
     gene            complement(1222..1899)
                     /locus_tag="PKW73_05260"
     CDS             complement(1222..1899)
                     /locus_tag="PKW73_05260"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNA72721.1"
                     /translation="MKKLITGLHHFQSHVFQSQKELFERLAQGQTPDALFITCSDSRI
                     NPNLITNTQPGDLFILRNAGNIIPPYGAANGGEGGTIEFAVSALGVKDIIICGHSACG
                     AMKGLLYPEQLDELPVVKAWLSHADATRRIVKENYELDKLEDDEVLSIAIQENVLVQI
                     ENLKTHPSVAAGLARGQLNLHGWAYKIQTGEVFTFNPKEGQFLPLTEVNYVREKRRPK
                     LNSGVSI"
     gene            2588..>3597
                     /locus_tag="PKW73_05265"
     CDS             2588..>3597
                     /locus_tag="PKW73_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72722.1"
                     /translation="MSSSKDPCAVLNAAEQAVTNRRFKTAVRKYAEYLKLTEPDPEML
                     LKIADVCQKADDNLQAIEYLEWASRLKPDDPKPLMDIARLCNSELKHNKALTALEAAL
                     AIAPPDKRILLQTLFTLREISVTSNRELSTEETYQKLIQAATLLIEIDPQCCKAYCYR
                     ALGRMRLNSQDLRGAQRDLDVALKLDSSEPLAHYYKALLHIEDGEFPAAFNLLERSFA
                     GFQKEGSDNFCGEISSLYHHYQDKAIWAKRQQYRGLPIEKQMQVLSECGIRILQNANP
                     EAILSEQRIRDFFEDEDPFHHLLIALVGAGLVSNGVQFDTECIYATGDYARFVKCFRE
                     LA"
BASE COUNT          971 a          953 c          860 g          813 t
ORIGIN      
        1 tgatggtggg agcgggtatg gcccagatcg gagaattctc gttcatcttg ctcagcatgg
       61 gccgggatct caaaatgata aacgactaca tgtacaacct cttctttgca ggagcggttg
      121 tgagcctgat tgccagcccg gcactatttg ccgccagtcc caagttgagt cactggctga
      181 gatttttggg cgcgtttttc aagaagaacc aaaacagtga ggaagaggaa ggccatgacg
      241 caagccgctt ggccggaaga ctcaaggatc atgtaattat ctgcggcttc ggcagagtcg
      301 gccgcaacct cggcacagtc ctttccagct acggcatacc ttacctggtc atcgaattga
      361 acgccggcat tatcgatgat ctggccggtc gtggtattcc gcatctttat ggggatgcca
      421 ctagcaacat gattatgatc aaagccaatc tcaaggaagc ggcctgcctg gtcttgacca
      481 tgccggatcc atcggcaatc acagccatta ctcgcttcgt gcgcagccgc aacgaaacca
      541 tcagtattat tgcccgcgca caccgccagg ccgacatcgc cgccttcacc gcctgtggtg
      601 tcagcgccat cgtgcaaccg gaattcgaag ccagtatcga aattaccaga ttggccctct
      661 tcagcaggcg ccgggctccg gaagaaatcg aagcggccct ggaaaaggta agagccatgc
      721 gctatcccca tcccagtccg gacttcgaag ccatggaccg ggcaccgctc tatatgatgt
      781 tggaagaaga acagatggga cgctggttcc tgaacgtgga cgacaatatg accgggaaaa
      841 gccctaaaga cttgaacgtg agaggactga ccggggtaac cattaccgct atcaagcggc
      901 aggaaaaaac acatacctat ccctctcccg acttcccatt cagcccggga gatcaaattt
      961 atgcagtcgg caaaaaagaa gagctagtgc gctttcagga gcacttcaag atgcgccaat
     1021 tttcgccggt accttagacc ggcgctcctt cagtcaaata gagacgccgc tataaaacac
     1081 atacctatcc ctctcccgac ttcccattca gcccgggaga tcaaatttat gcagtcggca
     1141 aaaaagaaga gctagtgcgc tttcaggagc acttcaagat gcgccaattt tcgccggtac
     1201 cttagaccgg cgctccttca gtcaaataga gacgccgcta ttaagcttag gacgacgctt
     1261 ttctctcaca tagttaactt cggtaagggg caggaattga ccttccttgg gattgaaggt
     1321 aaagacttcg cccgtctgaa tcttgtaagc ccagccgtgc agattcagct gcccgcgagc
     1381 cagcccggcg gccaccgacg gatgagtctt caaattctca atttgcacca gcacgttctc
     1441 ctggatggcg atactgagca cttcatcgtc ttccaacttg tccagttcgt agttctcttt
     1501 gacgatgcgc cgtgtggcat cggcgtggga aagccaggct ttcaccactg gcaattcatc
     1561 taactgttcg ggatagagca agcccttcat ggctccgcaa gctgaatgtc cgcaaataat
     1621 aatgtctttc acaccgaggg cgctgacggc aaattcgatg gtgccgccct ctccgccatt
     1681 ggcagcgccg tatggcggaa tgatattgcc ggcattgcgc agaatgaaaa ggtcgccggg
     1741 ctgagtgttg gttatcaaat taggattgat tctggagtcg gaacaggtaa tgaatagagc
     1801 atcaggggtc tgcccctgag ccagtctctc aaaaagctcc ttttgcgatt gaaatacatg
     1861 agactgaaag tgatgcagtc cggtaatcaa tttcttcatc aatcactcct taaagtgcct
     1921 gcgctcttgg ctggttctga aagaggagcc tgacttcaat ctgccgaggc caatgaagtc
     1981 tgatgcaaga ccgtcggagc caatcaatcg acatattcaa aatcttgcat atatgcatct
     2041 taacacaaga atgccccggg gagcaaggtg tgggcttgca gcgccggggc aattcagcac
     2101 ctcttccggc agacctacgg cgaggggcaa gctattttca aagtatcccg ccaggggtaa
     2161 agtcctggct gcggcttcaa ccagtcaggc caattgatct aaagccccga aaactcttgc
     2221 agttcttcaa atgggcagaa atgacatgca tcatccaaat acaaattgaa aaacttgatg
     2281 cgtttatcga gccatgtttc aaacattgcc aatgatattt aatgatcccc aagctttgcc
     2341 gaagcgtaaa agacccgacc tccggatcgg taggctcgcg ttcaccaacc acctacagtc
     2401 acaagagaat gctggagact ttgcctaacc tatctctgaa cgggctatag agcggcgccg
     2461 acacctattg acatcccaaa tgcagtcagc tatttctgac ttgtccttca agtcaatctg
     2521 cgccgtcaga gcagcgaaac aaattccagc ataagtaaac cctcaaatca ctctccgagg
     2581 taaccagatg tcgagctcaa aagacccatg cgctgtactc aatgcagccg aacaagccgt
     2641 gaccaataga agattcaaaa cagcagtaag aaagtacgcc gaatacttga aactcaccga
     2701 acctgatcca gaaatgctgc tgaagatcgc agatgtttgc caaaaggctg atgataatct
     2761 tcaggcaatt gaatacctag agtgggcaag cagactcaaa ccagacgacc ccaaaccgtt
     2821 aatggatata gccaggctct gcaattcaga actcaaacat aacaaggctc ttaccgcgct
     2881 tgaggccgcc ctcgcgattg ctccccctga caaacgaata cttcttcaaa cgctttttac
     2941 tctgagagag atctcggtta cgtcaaacag agaattatca acagaagaaa catatcaaaa
     3001 gcttattcaa gccgccacac ttctgattga aattgatcct caatgctgca aagcctactg
     3061 ttaccgagcg ctcggaagaa tgcgactcaa ttctcaggac ctcaggggcg ctcagcgcga
     3121 cctggacgtg gctctaaagc tagactcaag cgaaccactt gcccattatt ataaagccct
     3181 acttcacata gaggatggcg agtttccggc agctttcaac ttgctggaac gctcctttgc
     3241 cggcttccaa aaggaaggtt cggataattt ctgtggtgag ataagcagcc tctatcacca
     3301 ctatcaagac aaagcaatct gggccaaacg acagcaatat cgaggtctgc ctatcgagaa
     3361 acagatgcag gttcttagtg agtgtggaat tagaattcta cagaatgcaa accccgaagc
     3421 cattttaagt gaacaaagaa ttcgggactt tttcgaagac gaagatccat tccaccattt
     3481 gttgatcgct ctcgtcggcg cggggctggt atcgaatggg gtacagttcg acactgagtg
     3541 catctacgct actggcgact atgctcgttt cgtcaaatgt tttcgcgaat tagcaag
//
LOCUS       DAOQNZ010000222         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12425_length_3592_cov_4.036472, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000222 DAOQNZ010000000
VERSION     DAOQNZ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12425_length_3592_cov_4.036472"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PKW73_05270"
     CDS             complement(<1..824)
                     /locus_tag="PKW73_05270"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72723.1"
                     /translation="MPSSKMKSRPFADFAGFTVMAGLAGNAALSRVVALSLMLSLGFP
                     LAGLAYQDTGFDQGRARMMTGDYDGAVAAFGESLGLNSNNSRALVLRGECFFKMGNYK
                     LAIQDLTSALQYAPNNIRALILRGISHCSLGKDGMAILDFQSAIKLNGPLADKFFNRI
                     TGGGIAALERLEEQRKNDPSGAREEDSYDVDDESGFNVHAIKDYREALISLKEGRNST
                     IAPDDSITAQPTGGSHGGNSAVGTSNIGSASASGDGGSNPGRRRHPDWHKNAIAGTG"
     gene            complement(917..1411)
                     /locus_tag="PKW73_05275"
     CDS             complement(917..1411)
                     /locus_tag="PKW73_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNA72724.1"
                     /translation="MIKIARIVRLVALAILFGGGTGIVFAAITLVHAAKAQGIPVAEA
                     AATNAPIFLEFAKVAAAFAIALVVAEAIEIKGDLKNLEKRSKWPMARYFASIVAAVAA
                     FIFAFGIAPPMKDLQPKIKADQAAHAEFTRLHEVSRGVFSAMIVFAMASMIIPVLGPG
                     KHME"
     gene            complement(1572..2102)
                     /locus_tag="PKW73_05280"
     CDS             complement(1572..2102)
                     /locus_tag="PKW73_05280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNA72725.1"
                     /translation="MVALIEGSKAPDFQLKTSRDKVQSLGQALARFPLVLITIFKVHC
                     PVCQLTLPYIQRLHAGLKAAGAPDLPEEASPLPEESPLLVVCQDSQDETREFIEQYGL
                     TMPVLVDDNLDVTLAYGLTNVPSLFLVRADYTIESTIVGFDKSALISAAGRLAGDDFA
                     LFTSEENTTLPAFRPG"
     gene            2220..>3592
                     /gene="glgP"
                     /locus_tag="PKW73_05285"
     CDS             2220..>3592
                     /gene="glgP"
                     /locus_tag="PKW73_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNA72726.1"
                     /translation="MKPIRTIEVTPFLPPELECLRELAYNLRWTWDHETIKLFRRLDR
                     DLWDATGHNPVLLLGMVSQERLNEAAQDDGFVNQLNRICQKHYDYMKGEGTWYEKNFK
                     KSNAGVLAYFSAEFGLSDALPIYSGGLGILAGDHVKAASELGLPLVGVGIVYQQGYFR
                     QYLNADGWQQERYPINDFYNLPIIPVKEKDGSPVLVSVDFPGRKVYMRIWKAQVGRVP
                     LYLLDTNIPENSKQDEDITDALYKADREIRIQQEIILGVGGMRALKAVGIEPTICHMN
                     EGHSAFLGLERIAILMREKGLTFAEAKEVAAAGQIFTTHTAVPAGIDKFDPELVDKYW
                     SSYYHQVGLNRKDFFALGRTEPSDETELFSMANLAINLSSRTNAVSKLHGVVSRELFK
                     DNWKDVPEHEVPITHITNGVHTRSWISKEMADLFDRYLGPKWSEDISDQNIWKKVADI
                     PDEELWR"
BASE COUNT          760 a          935 c         1031 g          866 t
ORIGIN      
        1 ccggtgccgg cgatagcatt cttatgccag tcgggatgtc tgcgtctgcc ggggttactg
       61 ccgccgtcgc cggaggcgct tgcggagccg atgttggagg tgccgaccgc gctgttgccg
      121 ccgtggctac cgcccgtggg ctgtgccgtg atgctgtcgt cgggcgcgat ggtgctattt
      181 ctgccttctt tcaaacttat cagcgcttct ctgtagtctt tgatggcatg cacgttgaag
      241 cccgactcgt cgtcaacatc gtaagagtct tcttctctgg ctccgctggg gtcattcttg
      301 cgctgttctt ccaggcgttc gagggcggcg atgccaccac cggtaatgcg gttgaaaaac
      361 ttgtcggcca gggggccgtt cagcttgatg gccgactgaa agtcaagtat ggccatgccg
      421 tctttgccaa ggctacagtg gctgatgccg cgcagaatga gggcgcggat attattggga
      481 gcgtattgca gggcgctggt caggtcttga atagccagtt tgtagttgcc cattttgaag
      541 aagcactcac cccgcaagac cagggctctg gagttattgg agttgagtcc gagggattcg
      601 ccaaaagcag ccacggcgcc gtcatagtcg ccggtcatca tgcgggcgcg cccttgatcg
      661 aagccggtgt cctggtaggc aagaccggcc agaggaaagc ccagggagag catcaggctc
      721 agcgcaacca ctcggctcaa agccgcattt ccggcaaggc cggccataac ggtaaaaccc
      781 gcaaaatctg caaaaggtct ggatttcatc ttgctgcttg gcatttaatc tctctcgact
      841 gttcttggga actgactaca actcttcaca ggcttatttt cccggttttt caattacttt
      901 gcaccggtcg gggaaattat tccatgtgtt tgcccggccc cagaacggga attatcatgg
      961 aagccatggc gaagacaatc atggcgctga agacaccccg ggagacttca tgcaggcggg
     1021 tgaattcggc gtgggcggcc tggtcggcct tgattttggg ttgcaggtct ttcatgggcg
     1081 gggcgatgcc gaaggcgaag atgaaggcgg ccaccgctgc cacgatgctg gcgaagtagc
     1141 gggccatggg ccatttgctg cgtttttcca ggtttttcaa atcgcctttg atttcaatag
     1201 cctctgctac caccagggcg atggcgaagg cggcggctac tttggcgaac tccagaaaga
     1261 tgggggcatt ggtggcggct gcctcggcca cggggatgcc ctgggccttt gccgcatgta
     1321 ccagggtgat ggcggcgaag actatgcccg tgccgcctcc gaataggatg gccagggcta
     1381 ccagtcggac tatgcgggcg attttaatca tggttttttc cgttgtctta gtaagcggac
     1441 attttaaact gcgttgggac tacctggata tgcgttggat atgccggtcg actgccttgg
     1501 tctgttttga gtggtttcca gtggttggtg atttggatgg gctctaaacc ttagaacctg
     1561 acattggttc ctcagccggg gcggaaagcc ggcagggtgg tattttcttc gctggtgaag
     1621 agggcgaaat cgtcaccggc gaggcgcccg gccgcgctta tcagggcact tttgtcaaaa
     1681 ccgacaattg tgctctcaat agtgtagtcg gctctgacta gaaagaggct gggaacattg
     1741 gtgagaccgt aggctagcgt aacatccagg ttatcgtcca cgagaaccgg catggttagc
     1801 ccgtactgct caatgaattc cctggtttca tcttgcgaat cctggcagac taccagtagc
     1861 gggctttcct ccggaagcgg gcttgcttcc tcgggcaggt caggagcccc ggcggctttc
     1921 agcccggcgt gtaggcgttg tatataagga agtgtgagct ggcatacagg gcagtggact
     1981 ttgaagattg ttatgagcac aaggggaaat cgcgcaagtg cctgacccag tgactgtacc
     2041 ttgtctctgg atgttttgag ctgaaaatca ggcgctttag agccctcaat aagtgctacc
     2101 atttcaaagc cctcccgagg aaagatttta tcctcttggc agcgaggggt tgatgcggct
     2161 caaataggct gtatctgctc cttttattat gattgctatt ttgcagcgag gctacttagt
     2221 tgaaaccgat ccgcaccatc gaagtcactc ccttcctgcc gcccgagttg gaatgcctga
     2281 gagaactggc gtacaacctg agatggacct gggaccacga aaccatcaag cttttccgtc
     2341 gccttgaccg cgacctctgg gatgccaccg gtcataaccc ggttctgctt ctgggcatgg
     2401 tcagccagga gcgcctcaat gaagcagctc aagacgacgg ctttgtcaat caactcaatc
     2461 gcatttgcca gaagcactac gattatatga agggcgaagg cacctggtac gagaagaact
     2521 tcaagaagag taatgccggt gtgctggctt atttctccgc cgagttcggt ctttccgatg
     2581 cgctgcctat ttattccggc ggtctgggca tcttggccgg cgaccacgtg aaagccgctt
     2641 ccgaactggg tctgcccctg gttggtgtgg gtatcgttta tcagcagggc tatttccgtc
     2701 agtatcttaa tgctgatggt tggcagcaag agcgttatcc catcaacgat ttttataact
     2761 tgccgattat ccccgtgaaa gaaaaagacg gcagccccgt gctggtgtcg gttgatttcc
     2821 ccggacgcaa agtttatatg cgcatatgga aagcacaagt cggacgtgtg ccgctctatt
     2881 tgctcgacac caatattccc gagaactcca agcaagacga agacattacc gacgcgctct
     2941 acaaagccga tcgcgaaatt cgtatccagc aggaaatcat tctcggcgtc ggtggtatgc
     3001 gcgccttgaa ggctgtgggt atcgagccca ccatctgcca tatgaatgaa gggcacagtg
     3061 ccttcctcgg tcttgagcgc attgctattt tgatgagaga gaaaggtcta acctttgccg
     3121 aagcaaaaga agttgccgcc gccggtcaga tcttcaccac tcacaccgcc gtgcctgccg
     3181 gtatcgataa gttcgatccc gaactggtcg ataaatactg gagctcctat tatcatcagg
     3241 tcggcttgaa ccgcaaagat ttcttcgccc tcggacgcac cgagcccagt gacgaaactg
     3301 agctgttctc catggccaac ctggcaatca atctttcttc ccgcaccaat gccgtgagca
     3361 aactgcacgg tgtggtatcg cgggagcttt tcaaagacaa ctggaaagat gtgcccgagc
     3421 acgaagtgcc catcacccat atcacaaatg gcgtgcacac tcgctcctgg atctccaagg
     3481 aaatggccga tctcttcgac cgttatctgg ggcccaagtg gtcggaagat atctccgatc
     3541 agaacatctg gaagaaagtt gcggatattc ccgatgaaga gctgtggaga at
//
LOCUS       DAOQNZ010000223         3587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12454_length_3587_cov_3.578709, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000223 DAOQNZ010000000
VERSION     DAOQNZ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12454_length_3587_cov_3.578709"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1010
                     /gene="lipA"
                     /locus_tag="PKW73_05290"
     CDS             102..1010
                     /gene="lipA"
                     /locus_tag="PKW73_05290"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNA72727.1"
                     /translation="MNNKGDGVVEVEREEGQNPLPKPPWLKVKLPTGEPYWRVKNLID
                     EHKLHTVCESAACPNRGECWSRATATFMILGNVCTRSCGFCNVITGKPTELDLEEPER
                     VAEAVGRMNLNYTVITSVNRDELKDGGAAVWAATIEAVRQKSQCKIEVLIPDFMGNWD
                     NLEIVIRARPDVLNHNIETVPRLYLPVRPQGNYERSLVLLRRCKDAGMTTKSGLMVGL
                     GEEDEEVLSVLKDLKESGVDIVTIGQYMQPTPKHLPVARFVEPSRFAEYAAFGKELGF
                     ASVFSGPLVRSSYHADEVAREVGVTF"
     gene            complement(1043..2182)
                     /locus_tag="PKW73_05295"
     CDS             complement(1043..2182)
                     /locus_tag="PKW73_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNA72728.1"
                     /translation="MALKTPVKQGGTTSSKAGQTKANPGTQMKQGKVQAQTTASGKSI
                     FDAIPISLVLYLAKPYLAGETKEEALAIAHALYQENKYSSTLDILGEDASQDSDCDAS
                     VQHYKELIKLIADKPLALPESEARRQPTVSFKPSMFATKAPSVGMVRSAELDKAYNRI
                     ETLVGFAEGLGVRVTLEAEDHRWTDFHLETYFSLIESGYKNVGTVIQTRLFRTQKDLQ
                     RFDERMRTRVVIGIYNEPGTIAHTEKPIMKDLLVKYSRELLARGTYVEVATHDTQCLA
                     NFFAATVTDRVEPHRFETQYLQGVPRKRFQQALVSGQLFAGEEYKKVQEAQQFLANLA
                     EQGVLVRMYLPFGEGKVAGAYCRRRLKENPNMITYGIKNLLKLDS"
     gene            complement(2393..>3587)
                     /locus_tag="PKW73_05300"
     CDS             complement(2393..>3587)
                     /locus_tag="PKW73_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72729.1"
                     /translation="FTIENNTGKNLVGAPIQLQAHFRVLAEGILNIYRWQTYFDTNGG
                     QQLVNTEAHGKRPFELPMDQSEWPMIECKILCKLADGSPSQNLLVARVQPVAMTDDDA
                     RTQLNFLLGRTRLAKARMEAQKKKDQLQAAAAPKAKPKPEPPKQTPTGAGAANTTGSG
                     NNLSQPHPEPEKPLTAVAASMGNPLPLKGSELQALDLLVKGAATQAGLGDDFYHFEKA
                     MGMPVETDLQDKNWIWAQYRKQPAMRLYAGSKGRTGKADTIICILKQDTLSDSQIGSA
                     VKALAGKFKSEKINAAEHSVRYVDNSLSSSGRLEFVVINGQSFRGLSFKLNDTDGARA
                     TAVVVSRLPGALGEQLKEEARKTNILGFLLTGLGESAQRQPSTDGDRARRSGPTYGGN
                     DDSDQ"
BASE COUNT          722 a          917 c         1038 g          910 t
ORIGIN      
        1 ccagggaaag tatgtcatat attgatgccg tgatcttgtt ttgcgctgtc taaattcttg
       61 ttaggcgcgg tctaaaaccc agttgtgggg cgaaaagctg aatgaataat aaaggcgatg
      121 gtgtggtcga agtagaacga gaagaggggc aaaacccgct tcccaagccg ccctggctca
      181 aggtgaagtt gcccacgggt gagccctact ggcgggtcaa aaatctcatt gacgagcata
      241 aactgcacac tgtgtgcgag tcggccgcct gtccgaaccg gggcgaatgt tggtcgcggg
      301 ctacggccac ttttatgatc ctcggtaatg tctgcactcg cagttgcggc ttttgcaatg
      361 taattaccgg caagcccacc gagcttgatc tggaagaacc tgagcgagtg gctgaggccg
      421 tcggccgcat gaacctcaat tacacggtta tcacttccgt caatcgcgac gaattgaaag
      481 acggcggtgc ggccgtctgg gccgccacca tcgaagctgt caggcagaag tcccagtgca
      541 aaattgaagt cttgattcca gacttcatgg gcaactggga caacctggaa atagtgataa
      601 gagcccgtcc cgatgtactc aatcacaaca ttgaaaccgt gcccaggctc tatttgccgg
      661 tgcgtcccca gggcaattat gagcgttccc ttgtcctttt gcggcgctgc aaagacgccg
      721 gcatgaccac caagtcgggg ctcatggtcg gtctcggcga ggaagatgag gaagtgcttt
      781 cagtcctgaa agatctgaaa gagtccggcg tggatatcgt caccatcggc cagtacatgc
      841 agccaacgcc caaacacttg ccggtggctc gttttgttga accatcccgt ttcgccgaat
      901 acgccgcctt cggaaaagaa ctgggttttg cctcagtctt ttccggtccg ctggtgcgca
      961 gtagttacca cgccgatgag gtggcgcggg aagtgggtgt aaccttctga tttaagaccg
     1021 gagggaactt tgccgaaata aactaggaat ctagtttgag caggttcttg atgccgtagg
     1081 taatcatgtt cggattttcc ttcaggcggc ggcggcaata agctccggcc accttgcctt
     1141 cgccgaaggg cagatacatg cgaaccagca ctccttgctc ggccagattg gcaagaaatt
     1201 gctgggcttc ctgcactttc ttatactctt cgccggcaaa tagttgacca ctgaccaggg
     1261 cttgctggaa acgcttacgc ggcacacctt gcaaatactg cgtctcgaag cggtggggtt
     1321 ccacacggtc ggtgactgtg gcggcgaaga aattggccag gcattgtgtg tcgtgggtgg
     1381 ctacttccac ataggttccc cgggccagta gttcgcggct gtacttgacc agcaggtctt
     1441 tcattattgg tttttcagta tgggcgatgg tgcctggttc gttatagatg cctataacca
     1501 cccgggtgcg catgcgttcg tcgaagcgct gtaaatcttt ttgagtgcgg aagagcctgg
     1561 tttgtatgac ggtgccgacg tttttatagc ccgattcaat cagtgagaaa taagtctcaa
     1621 ggtgaaaatc ggtccagcga tggtcctcgg cttccagcgt cactctcacg ccgaggccct
     1681 cggcaaaacc caccagggtt tcgatgcgat tgtaggcttt gtccaattcg gcgctgcgca
     1741 ccatacccac gctcggggct ttggtggcga acatggaggg cttgaaggag acagtcggct
     1801 ggcgcctggc ttcgctctcg ggcagtgcga ggggcttgtc ggctatgagt ttgatcagct
     1861 ctttgtagtg ctgcaccgaa gcatcgcagt cactgtcctg ggaggcgtct tccccgagta
     1921 tgtcgagggt ggaggaatac ttgttttcct ggtagagggc atgggctatg gccagggctt
     1981 cttcctttgt ttctccagcc agataaggtt tagccaggta gagaaccagt gaaatgggga
     2041 tggcgtcgaa gatagatttg ccgcttgccg tagtctgcgc ctggactttc ccttgcttca
     2101 tttgagttcc cggatttgcc ttcgtctggc cggctttcga acttgtggtg ccgccttgtt
     2161 tgactggtgt tttgagagcc ataaagtggt cttgaatccc ccgaggagta aattccgact
     2221 ggttttcaac agggattata gaaccaatgt cagtcaaaaa tataaacaaa aatatagaca
     2281 atgtcacaat gacctttgtc ctgaattttc taatttgccg tctattggcg gcagctatag
     2341 aaaaacgccg ttttctcggg agcggcggcg acttaatttc ttatcagaat gctcactgat
     2401 cggaatcgtc gttgccgccg tatgtagggc cggaacggcg agccctatcg ccatcggtgg
     2461 agggctgacg ctgcgcgctc tcgcccagac cggtgagaag aaagcccagg atattggttt
     2521 ttcttgcctc ttccttgagc tgttcgccca gggctccggg caggcgactg accactacgg
     2581 ccgtggctct ggctccgtct gtgtcgttca gtttgaagct cagaccccgg aaactctggc
     2641 cgttgatcac gacgaactcc agacggccgc tgctggataa actgttatcc acgtagcgca
     2701 cggaatgttc ggcggcattg attttttcgc ttttgaactt gccggcaagc gccttcacgg
     2761 ctgaaccaat ctggctgtcg ctcaaagtat cttgtttgag aatgcaaatg atggtatcgg
     2821 ctttaccggt acgtcccttt gatccggcat agaggcgcat ggccggttgc ttacggtact
     2881 gggcccagat ccagttcttg tcttgcagat cggtctccac aggcatgccc atagcctttt
     2941 caaagtggta gaagtcatct cccagaccgg cctgagtggc ggctcctttc accagtaaat
     3001 cgagggcctg caactcgctg cccttgaggg gcagggggtt acccatgctg gccgccaccg
     3061 ctgttaatgg cttctccggt tcggggtgag gctggctgag gttattgcca ctgccggtgg
     3121 tgttggcggc gccggcccct gtgggcgttt gtttcggcgg ctccggtttg ggtttggctt
     3181 tgggggcagc ggccgcttgc aactggtctt ttttcttctg ggcttccatt ctggcctttg
     3241 ccaggcgggt gcgccccagt aagaagttaa gctgggtgcg ggcgtcgtca tcggtcatgg
     3301 ctaccggctg cactcttgct accagcaaat tctggctggg ggagccgtcc gccagcttac
     3361 acaaaatctt gcattcaatc atgggccatt cgctttgatc catgggcaat tcgaaaggtc
     3421 gcttgccgtg ggcttccgta tttaccagct gctgtccgcc attggtgtcg aaataggtct
     3481 gccagcggta aatgttgaga atgccttcgg ccagtacacg aaaatgagct tgcagttgaa
     3541 tcggtgcacc aacaagattt ttgccggtgt tgttttcgat agtgaag
//
LOCUS       DAOQNZ010000224         3581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12487_length_3581_cov_3.344867, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000224 DAOQNZ010000000
VERSION     DAOQNZ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12487_length_3581_cov_3.344867"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PKW73_05305"
     CDS             <1..617
                     /locus_tag="PKW73_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72730.1"
                     /translation="ELSEKSLMKASRQGIESMDLHWQLAHLYRAQGKDRQALAEINRA
                     LSLSPDSIELYNDRSSIYKDRGEHLNAANDCSKAISIMESQHLPPAAMFIMKDRRAGE
                     YIKAGLYDKAIVDLEFLLAKEKKNEGRRQNLIGECLVAQGKLKPALSRFDAAIKGSPL
                     YLDARRNRLKVLNMLKDEKGAAKERRQIENIEKDFSPFDSMKPQ"
     gene            complement(693..2348)
                     /locus_tag="PKW73_05310"
     CDS             complement(693..2348)
                     /locus_tag="PKW73_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72731.1"
                     /translation="MGKEVEEVKPKSKAQEAADKTVDETKKQLETTPGGDTHGAYNKL
                     AESVRQTHQELDKGDKTGKTSKEYEEAVVKKLQEEKLLPVLALEYGQRAFEKIDHKSA
                     DSGTGNGHLTKEELKAFKEAGRPGVFGKAMVDSLIDQSSSLEAKPAGGRGVKPAGISQ
                     VDLADGVIKHENAREAKISNEKKAEEEKVVHGEAKQLQSYLFEGKDGKPSLFAQADFA
                     GSSDIKNRSDGKVGKHDFEALATDPRLSEQEKKELKEKVLDKWDDPKFQSRYLENGYM
                     SADTFKKATGEEQAAKEQEAQKQAEAKNKELENRKKTEEEQAAKTKTEASGLQDLLSK
                     KIGNANVHEAADYAGSDNKDARSDKLVNKNDLKALLSSPDVDPALAKEIRENIVDKMD
                     SADFKKKYMSGDYLDLEKIKKATGVQDAVEAEDQKWKDIAELGKETKQREESDAKARQ
                     IALEREQAVQLATELVRNDGSGSLHARADFVGSSDAKNRTDKLVGKNDLEALVADPSL
                     SEEKRKFIQDNFLGDKWNSPAVKRMRQGNYISPESLKALLPNS"
     gene            complement(2610..3473)
                     /locus_tag="PKW73_05315"
     CDS             complement(2610..3473)
                     /locus_tag="PKW73_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA72732.1"
                     /translation="MPQVLFRSFLESYPELLAALALALFLAVKSAFAKNRDNSGPIYI
                     PVYLVSPAAEILKFFPNYLQGLQLKLNYCGLRGNTQLGVLAAAKVYPALLTPLAGMFI
                     NHPLILTGLAAFLFFAPDLVLTMLARRRQASIRESLPELLDLMVLCVDAGLGLDATLS
                     RVAQDPASGNSALLDELLTLNRDILLGMSRERAFQELYKRTGVEELGAFAGSLNQTSQ
                     LGLSISKTLRNQAEFLRSKLSQKAEEKAARLPIYMAFPLWFCIMPALMVLVLAPSLLI
                     FFEQVKPGFGL"
BASE COUNT          731 a          900 c          928 g         1022 t
ORIGIN      
        1 tggagctttc ggagaagtcg ctcatgaaag ccagtcggca gggtatagag agcatggatc
       61 tacactggca gttggcccat ttgtacagag cccagggtaa ggatcgtcag gcccttgccg
      121 aaatcaaccg ggccctttca ttgagcccgg attccattga gctttacaac gatcgttcct
      181 caatctataa agatcggggt gagcacctca atgccgccaa tgactgcagc aaggctatta
      241 gcatcatgga aagccagcac ctgccgccgg ccgccatgtt catcatgaaa gaccggcggg
      301 ccggtgaata tatcaaggcc ggtctctatg acaaggctat tgtagattta gaatttttgc
      361 ttgccaagga aaagaaaaat gagggaagaa ggcaaaacct gattggagaa tgtttggtgg
      421 ctcagggtaa actcaagccg gcgctttccc gttttgatgc cgctatcaaa ggctcacctc
      481 tttatctgga cgcccgaagg aaccgcctta aggttttaaa catgctcaag gatgagaagg
      541 gtgcggcaaa agaacgtagg caaattgaga atatcgaaaa ggatttcagc ccttttgaca
      601 gtatgaagcc ccagtgaata gaccttagta aggtacctcg ccaggaggaa atcttgtggc
      661 gaggtacctg gttgttaggg gcgaccctat gcttagctgt tcggtagcag cgctttcagc
      721 gactcaggcg agatgtagtt gccctgtctc attcttttca ctgccggcga attccacttg
      781 tctcccagga aattgtcctg gatgaatttg cgcttttctt cgctcaagct gggatcggca
      841 accagagcct ccaggtcgtt tttgcccacc aatttgtcgg tgcgattttt tgcatctgaa
      901 gatccgacga aatcggctct ggcgtggagc gatccgctgc catcgtttct gaccaactct
      961 gtggccaatt gcacggcttg ctctctttca agggcaattt gcctggcttt ggcatcgctt
     1021 tcctctcttt gtttggtctc cttgccaagc tcggctatgt ctttccactt ctggtcttca
     1081 gcttctacag cgtcttgaac acctgttgct ttcttgattt tctccagatc caggtagtcg
     1141 ccgctcatgt attttttctt gaagtcggcg ctgtccattt tgtctacaat gttctcccgg
     1201 atctctttgg ccagggccgg atctacatcc ggtgaggata gcagcgcctt caggtcattt
     1261 ttgttgacta gcttgtcgct gcgtgcatct ttgttgtctg aaccggcgta gtcggccgct
     1321 tcatgcacat tggcattgcc tattttcttg ctcaacagat cttgcaaacc ggaggcttcg
     1381 gttttggtct tagcggcctg ttcttcttcg gttttcttcc tgttctctaa ttctttgttc
     1441 ttcgcctcgg cttgtttttg agcttcctgc tctttagccg cttgctcctc tccggtcgct
     1501 ttcttgaagg tatcggcgct catatagcca ttttccagat agcgactctg gaatttagga
     1561 tcatcccatt tgtccaggac tttctctttc agttcttttt tctcttgctc tgagagtctg
     1621 ggatcggtgg ccagggcttc aaagtcatgc ttgcctactt ttccgtcgct cctgttcttt
     1681 atgtcactgg agccggcaaa gtcggcctgg gcaaagagag agggttttcc gtcttttcct
     1741 tcaaatagat agctctgtag ttgcttggct tctccatgta ctactttctc ttcttctgct
     1801 ttcttctcat tgcttatctt tgcttctctg gcattctcat gtttgatcac gccgtcggcc
     1861 agatctacct gcgaaatacc tgctggcttg actcccctgc cgcctgccgg ttttgcctcc
     1921 agggaactgg attgatcgat gagagagtca accatggctt tgccgaaaac ccccggtctg
     1981 cctgcctctt tgaaggcttt cagctcttct ttagtgaggt ggccgttgcc cgtgccgctg
     2041 tcggcgctct tgtggtcgat tttttcgaaa gctctctgtc catactccag agcaagaaca
     2101 ggcagcagtt tctcttcttg cagcttcttc accactgctt cttcatactc tttagaggtt
     2161 ttgccggttt tatcgccctt gtccagttcc tggtgggttt gccgaaccga ctcggccagc
     2221 ttattgtagg ccccatgggt gtctccgccc ggtgttgtct caagctgttt cttggtttca
     2281 tctaccgtct tatcggcggc ttcttgagcc ttgctcttgg gcttcacttc ttctacttct
     2341 ttacccatga cttttcctcc tgcctagaaa aggctagcga aatgttcgct tgtttctgaa
     2401 tgtgttaatt ggttttgggg tttttaacca gggcaaaata ggctccctct caagaggaag
     2461 acgctcgtgc accaggcatt ggatcttggg aatttagggg tggctgtgag cttgttgcgt
     2521 tgctcacatt gccaagatag tttttcttct gtgacaactg aatacaattg ccatgggagg
     2581 attcccatag ccagagctgt ttcggttaat taaagcccga agcctggttt tacttgttcg
     2641 aagaaaatca gtagagacgg agctaagacc agcaccatca gggccggcat aatgcaaaac
     2701 cagagcggaa aggccatata gataggtagg cgcgctgctt tctcttccgc tttctggctt
     2761 agtttggagc gtaggaattc ggcctggttg cgcagggttt tggaaatact cagtcctagc
     2821 tggctggtct gattgaggga gccggcgaag gcacccagct cttccacgcc ggtgcgtttg
     2881 tagagttctt gaaaggcgcg ttctcggctc atgccgagca agatatcgcg gtttaatgtc
     2941 agtaattcat cgagtagggc ggaattgccg ctggccggat cttgagcgac ccggctcaaa
     3001 gtggcatcca gaccgagacc ggcatccaca caaaggacca tcaaatccag cagttccggt
     3061 agggactctc tgatggaagc ctgccttcgt ctggccaaca tggtcagcac cagatccggt
     3121 gcaaagaaga gaaaggcggc cagcccggtg agaataagag gatggttgat aaacatgccg
     3181 gctagaggag tcaggagggc tggatagact ttggctgccg ccaagacacc gagctgggta
     3241 ttgccgcgta gtccgcagta gtttaatttg agttgcaaac cttgcagata attggggaaa
     3301 aacttgagaa tttccgccgc cggactgacc aggtagaccg gtatgtagat tggcccggag
     3361 ttgtcgcggt tctttgcgaa agccgacttg acggctagaa agagcgccag ggcaagggct
     3421 gctagaagtt ccggatagct ctccagaaag gagcggaaaa gtacttgagg catcaggctt
     3481 ttacctccgt caacttcttc atgagaaaca tgcccagcac ctggagaaag gcagccagca
     3541 tgagcagcag tttgccggta tccttctgaa agagagggcg c
//
LOCUS       DAOQNZ010000225         3572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12530_length_3572_cov_4.344043, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000225 DAOQNZ010000000
VERSION     DAOQNZ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12530_length_3572_cov_4.344043"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..2403
                     /locus_tag="PKW73_05320"
     CDS             136..2403
                     /locus_tag="PKW73_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72733.1"
                     /translation="MAREDKGSAAGSYQDTASGKSGEQDTGSLAARRARLRGTLSKQI
                     TPPDPYQPSPSETYQPGSTSSLSTEAKESEDPSTAGADSEESIAEGHSNGGNGNLATD
                     LAEAPSAEVGAAESPFFAAGSGKSMDFDTSRLFKDDDDDLKDAGSTAIPVKEAKDQTQ
                     NDEKQGENLAQKQAPIPEKVDSPTSAAPVPVLMAPTVVNVDNPQVVELLNNLDQQLGI
                     CSVNLSQIAKAANEQLDLVRNLSETIQHQAFGEIGLSLNSLSESLSAALEPMKAVGEL
                     VPAIDTLVTTIGSNAEADRRKAEEVKEDTRHTPEMLVTSLADQLASGRIDPWTFKCAY
                     MAVFPTEHPADLLRKLVDLLGTQKLSGDLFRAAYDAVQAPDPPKPVYNYQQAAGQNPE
                     GVTEVRYVQDEAVLAKLEELRKANEELKGRMDQRELEFSELLQSKEQEVAETQEMLNS
                     RWEDFSGRYEQLSEMVRERNEELQKKETELARIQAEKDLELARLQSDRDLEMSRVQSE
                     IANKDTEISQLKSQLEEMRDQTKDMVADLQRQLTSTKLAVEEAARLSANPPPKPQPQP
                     GFFESVASQSNTGNNSNPAASLFNSEPAAGAMFNASESGSVAAAPQAVPAPVPVPVPV
                     PVPVPVPVPTPAVSASVGAPSQALVGSASPMVSAPDPIVPSPNPAVAAAAAAAAAGQA
                     IPRPQVAAPTTPFPGSPGSYGSGVRAQVFEVIVRQALAGAPWREICAGPMTVNGISPD
                     EVEAEVKRRQALLKK"
     gene            complement(2410..2652)
                     /locus_tag="PKW73_05325"
     CDS             complement(2410..2652)
                     /locus_tag="PKW73_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72734.1"
                     /translation="METSQLEAKIDQLSKRVDEQMRISRNLTALCTTVIIGVMFFIFT
                     QTIQHLPAIIVFHYMSNLDAIVKEWHQAERALKLTN"
     gene            complement(2784..>3572)
                     /locus_tag="PKW73_05330"
     CDS             complement(2784..>3572)
                     /locus_tag="PKW73_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72735.1"
                     /translation="GEQTGMLLKCHRSNYQIVGFTEETEAAELSALGKEMSVPVVFDL
                     GGGSFVDLRQFDLKPEPTVMETLADGIGLVLFSGDKLLGGPQAGIICGKAELVARLRK
                     CPIYRALRADKVVLALIEAALSQYLYNNGYQALPVFQFACRSVESLQTRAEQIVAGLQ
                     KPGLQALQVAVCPMQSTMGGGSLPGEVQDSRGLCLTQSGKSKKESKSAEAIASFLRGY
                     SVPIISTIAKEAVYLDLRAVFQQEDQIIEAALKALDEHLQEPPH"
BASE COUNT          804 a          975 c         1000 g          793 t
ORIGIN      
        1 actggaagag tggggttcac tgcggcgagc gtggggttcg gcaatgaacc ggcagatcca
       61 gcctcgagtg tgaactaggg cggttctggg tacatatttg acagtgaaca ggggacattg
      121 ggaagggttt actagatggc tcgcgaagat aaggggagcg ccgccggatc ttatcaagac
      181 accgcttctg gaaagtctgg cgaacaggat accggctctc ttgccgctag acgggctcgc
      241 cttagaggca ctctttccaa gcaaataacc cctccagatc cctaccagcc ttctccttcc
      301 gagacttatc agcctggcag cacttcctct ttgagcactg aagccaagga aagtgaggac
      361 ccttctactg ccggtgcaga ttcggaggag tctattgctg aaggacattc caacggcggc
      421 aacggcaacc tcgccacaga cctggcggaa gcgccttcag ccgaagtcgg tgccgctgag
      481 agcccgtttt ttgccgcagg ttcaggcaag tcaatggatt ttgatacctc tcgtcttttc
      541 aaggacgacg atgatgattt gaaggatgcc ggctccactg cgattcctgt taaagaagca
      601 aaagaccaga cgcagaacga cgaaaagcaa ggtgagaatt tggctcaaaa gcaggctcca
      661 attcctgaga aggtcgacag cccgaccagc gccgctccgg taccggtgct catggcgccc
      721 actgtggtga atgtggacaa cccgcaggtg gtagagctac tgaacaatct tgatcagcag
      781 cttggtatct gttcggtaaa tctttctcaa attgccaaag ccgccaatga acagcttgat
      841 ctggtgcgca atttatccga aaccattcag catcaggcct ttggtgagat tggtttgagt
      901 ttgaacagtt tgagcgaatc gctttcagcc gcccttgagc ccatgaaagc tgtcggcgaa
      961 ttggtgccgg ctatcgatac acttgtgacc accatcggtt ctaatgccga agctgatcgc
     1021 cgcaaggcgg aagaggtgaa agaggatacc cgtcacacgc ccgagatgct tgtcacctcg
     1081 ctggccgatc agttagcgag cgggcgcatt gatccctgga ctttcaaatg cgcttacatg
     1141 gcagttttcc cgactgagca tccggccgat ctcttgcgta agttggttga cttgctcggt
     1201 acccagaagc tttcaggcga tctattccgg gcggcctacg atgccgtgca ggcacccgat
     1261 cctcccaagc ctgtctataa ctaccagcag gcagcaggtc aaaatcctga aggcgtcact
     1321 gaagttcgct atgtacagga tgaggcggtg ttagccaagc tcgaggaatt gcgcaaggcc
     1381 aatgaagagc tgaaaggtcg catggaccag cgagagcttg agttttccga actcttgcag
     1441 tccaaagagc aagaggttgc cgagacccaa gaaatgctca attcgcgctg ggaagatttc
     1501 agtgggcgct acgagcaact ctcggaaatg gtgcgtgagc gcaacgagga gttgcagaag
     1561 aaggaaacgg aactggctcg cattcaggct gaaaaagatc ttgaactggc tcgtctgcag
     1621 tctgaccgag atctcgagat gtcccgggtg caaagtgaga tagccaataa agacaccgag
     1681 attagtcaac tcaaatcgca acttgaagag atgcgggatc aaaccaaaga catggttgca
     1741 gatctgcaaa gacagctgac cagcaccaag ctggccgtgg aggaagcagc cagactgagc
     1801 gccaatcctc cgcccaagcc tcagccgcag cccggtttct ttgagtcggt ggccagtcag
     1861 tctaatacgg gcaacaacag caaccctgcc gcatcgctgt tcaatagcga accggcggct
     1921 ggagccatgt ttaatgcctc tgaaagcggc agtgtcgccg ctgcaccgca ggcggtgccg
     1981 gctccggttc ctgttcctgt tcctgttccc gtaccagtgc cggtaccagt ccccactcct
     2041 gccgtcagtg cctcggtcgg agctccgtct caggcgcttg tcggcagcgc ttcccctatg
     2101 gtgagtgctc ccgatcccat tgtgccttct cctaatccgg ctgtagcagc tgctgccgca
     2161 gcagctgccg ccggtcaggc catcccccgt ccgcaagtgg ccgcaccgac tactcccttc
     2221 ccaggcagtc ccggtagtta tggcagcggt gtgagagcgc aggtctttga ggtgatcgtg
     2281 cgtcaggcct tagccggtgc cccctggcga gagatctgtg ccggcccgat gacggtgaac
     2341 ggcatctctc ccgatgaggt ggaggcggaa gtgaagcgcc gacaggcgct gctcaaaaag
     2401 tagagtaaat cagttggtta gcttgagggc tctctcagcc tgatgccact ctttcactat
     2461 ggcatcgaga ttgctcatgt agtgaaagac tattatggcc ggcaggtgct gaatagtctg
     2521 ggtgaagatg aagaacatga ctccgataat gaccgtggtg cagagcgccg tcagattgcg
     2581 actgatgcgc atctgttcgt cgactcgctt gctcagttga tcaattttgg cttccagctg
     2641 tgaagtttcc acatttatca ccatagtcgg caagtttggt cggcggttgc aatattgcct
     2701 gagggagcgg aagtcaaaaa tagcacatct atataagagt tgcttttaat gacaaccggg
     2761 cgctacagtc tctgatggcg gggctagtga ggcggttcct gcaaatgctc gtccagagct
     2821 ttcaaggctg cttctatgat ctggtcttcc tgctggaaaa cggctcgcag gtctaagtag
     2881 accgcttctt tggcaatggt tgagataatc ggtacgctgt atccgcgcag gaaggatgct
     2941 attgcttccg ccgatttgct ctctttctta gatttgccgc tctgggtcag gcaaagtcct
     3001 ctactgtcct gtacctcgcc aggcaggctg ccgcctccca tggtactttg catggggcag
     3061 acagccacct ggagagcctg caagccgggc ttttgtagtc ccgctacgat ctgttcggct
     3121 cttgtctgaa ggctctcgac actgcggcag gcaaactgaa agaccggtaa agcctgatag
     3181 ccgttgttat acaaatattg actcagcgcc gcttcgatca aggcgagcac tactttgtcg
     3241 gcgcgcagcg ctctatatat aggacatttc cgcagccttg ccaccaattc ggctttgccg
     3301 cagatgattc cggcctgggg accgcccaag agtttgtccc cggagaaaag caccagacca
     3361 atgccgtcgg ccagggtttc catcacggtt ggttccggct tcaaatcgaa ctgtctcagg
     3421 tctacaaagg aaccgccgcc caggtcgaag accacaggca cggacatctc tttgccaagc
     3481 gccgaaagct ccgcagcttc cgtttcctcc gtaaagccca cgatctgata attggaacgg
     3541 tgacacttga gcagcatgcc ggtttgctcg cc
//
LOCUS       DAOQNZ010000226         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12588_length_3564_cov_3.255343, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000226 DAOQNZ010000000
VERSION     DAOQNZ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12588_length_3564_cov_3.255343"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKW73_05335"
     CDS             <1..969
                     /locus_tag="PKW73_05335"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNA72736.1"
                     /translation="PLLQRPPIRAGRGPGRREIGHGALAERAIIPALPSHEEFPYTIR
                     VVSEVLESNGSTSMASTCGSSLALMDAGVHLKTTIGGIAMGLILEGDRFAILSDIQGL
                     EDFLGDMDFKVTGSRDGITALQMDIKIEGISIEIMRVALEQAKRGRIHIIDKMEAALP
                     GPRKELSTWAPRIITINIPQEDIGTVIGPGGKMIRRIIEETGATIDIEDDGTVLIGSV
                     DSAGGEAARDWIVRLVKKVQVGGVYIGKVTRIIPAGAFVEILPGKEGLVHISQLENRR
                     VEKVEDVVSIGAKVIVKVKEIDEKNRVNLTMKGVSPEEREACEKNG"
     gene            953..1768
                     /locus_tag="PKW73_05340"
     CDS             953..1768
                     /locus_tag="PKW73_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72737.1"
                     /translation="MRKTAKFRTLSLLLALVMAFAAITDCHARSSSKARGTGARKTTA
                     RRSASGRKKVSTSRGKKYARARVHRKGASATALNSRKRRFVPEKPSQRIEPVDTVSDS
                     EPRRRNYSLLSQAYQLYDQALSQEIIGNYGASLDKLTEALSLLDQARSHQRQGVPSTM
                     EAMGFFELGRVAEADGDFRLARDSYSHCWKASPQFVEAYVCGSLLQARLGNLELARAL
                     ALEGKNSVGADERLETLLKSLNARLGPLPASQPAGVDGGAAGGEGEIQPQFEP"
     gene            complement(1883..2164)
                     /locus_tag="PKW73_05345"
     CDS             complement(1883..2164)
                     /locus_tag="PKW73_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNA72738.1"
                     /translation="MNKAELAAEIAARTNTTKKSVEEMLAAFTDVVTEVVAKGERVTL
                     VGFGTFLRRDRQARETNNPQKPGEKIKVPAKRVPAFTPGKEFKDIVDHK"
     gene            2531..2959
                     /gene="rsfS"
                     /locus_tag="PKW73_05350"
     CDS             2531..2959
                     /gene="rsfS"
                     /locus_tag="PKW73_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNA72739.1"
                     /translation="MEVGTNKESDLAGVLLSSSTNTPVVDSQAAAYLAANACESKKAK
                     STTVLDVSKVTLLADYFVIAGAESAAQVRAIVDAVDDTLRQAGFKPKSIEGKTEARWV
                     LLDFGHVIVHVLQEKERNYYKIEQFWNHALIVDREEWVQE"
     gene            3015..>3564
                     /locus_tag="PKW73_05355"
     CDS             3015..>3564
                     /locus_tag="PKW73_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72740.1"
                     /translation="MSFRTRLQGFVDSRQKIQIWYGMGCHNTVTGRVLTVHPDHIDVE
                     SYSHESDGQIHVRRILIPLHLILHIDITSAEISEEDETNFSTEKDGKSGAPTNVIDLP
                     DAPRGYAVRVLSQLNSAFSNRFSRMTAGPGGIYFSCDGSVWFIDGNGALSEYARIRGN
                     STISGLRFDRKGVLYVATIQGTV"
BASE COUNT          821 a          922 c          973 g          848 t
ORIGIN      
        1 ccccttctgc aacgcccgcc aatccgcgcc ggccgcggtc ccggtcgccg ggaaatcggc
       61 catggtgccc tggctgaaag agctatcatt ccggctctgc ccagccatga agaatttccc
      121 tacacaatcc gcgtggtttc ggaagtgctc gagtctaacg gctctacttc tatggcttcc
      181 acctgcggtt cttccctggc tctcatggat gccggtgtgc acctgaaaac aaccatcggc
      241 ggtatcgcca tgggtctcat tctggaaggg gaccgctttg ccatcctctc cgatattcaa
      301 ggtctcgagg acttcctcgg cgacatggac ttcaaggtga ccggttccag agacggaatt
      361 accgccttgc agatggatat caaaatagag ggtatttcca tagaaatcat gcgggtggcc
      421 cttgagcagg ccaagcgcgg tcgtattcat attatcgaca agatggaagc ggctctgccc
      481 ggacctcgta aagagctgtc cacctgggct cctcgcatca taaccatcaa cattccgcag
      541 gaagatatcg gtaccgtaat tggaccgggc ggtaagatga tccgtcgcat cattgaagaa
      601 accggcgcca ccatcgacat tgaagatgac ggtacggtct tgattggttc tgtggattca
      661 gccggcggtg aagctgcccg tgattggatc gtgcgcctgg taaagaaagt tcaggtggga
      721 ggcgtttaca tcggtaaggt gacgcgcatc attcccgctg gtgctttcgt ggagatcctg
      781 cccggtaagg aaggcttggt acatatttct caattggaaa accgcagggt ggaaaaagtt
      841 gaggatgttg tatccattgg cgccaaggta atcgtcaagg tcaaggaaat tgacgagaaa
      901 aatcgcgtca acctgaccat gaaaggtgtc agcccggaag aacgcgaagc ctgtgagaaa
      961 aacggctaaa ttcagaacct taagcctgct tctggcatta gtgatggcgt ttgccgccat
     1021 cactgattgc catgcacgta gctcaagcaa ggccaggggc actggcgcca ggaagacaac
     1081 ggcaaggcgc tcggcaagcg gccggaagaa agtttcgacc agccgcggca agaagtacgc
     1141 ccgcgcccgt gtgcatcgca agggagcaag cgctaccgcg ctcaattccc gcaagcgccg
     1201 ctttgtaccg gagaaaccct ctcaaagaat tgaaccggtg gatactgtca gcgacagcga
     1261 accgagacgg cgcaattatt ccttgctttc gcaagcctac caactctacg atcaagccct
     1321 cagtcaggag atcataggta actatggcgc ctctcttgat aagcttacgg aggccctcag
     1381 cctgctcgac caggcccggt cccaccagcg ccagggcgtg ccctccacta tggaagccat
     1441 gggctttttt gaacttggcc gggtggcgga ggccgacggc gatttccgtc tggccaggga
     1501 tagctactct cactgctgga aagcttcgcc gcaatttgtg gaggcctatg tttgcggctc
     1561 cctcttgcag gctagattgg gcaatttaga actggcccga gccctggctc tggaagggaa
     1621 aaatagtgtc ggcgccgatg agcgcctgga aaccctgctc aagagcttga atgcccgtct
     1681 tggtcccctt cctgccagcc agcctgcagg ggttgatggg ggtgccgccg gcggtgaggg
     1741 agaaattcag ccgcagttcg agccgtagtg cgagtgcctg gtttgccgtg ggacgggctg
     1801 tttgaagata tctaaaagaa agagaggagc tcttttggga gcacctctct ttcttttggg
     1861 ttcttagttt gcgaccggca aattatttgt ggtcgacaat atccttgaac tctttaccag
     1921 gtgtgaaagc cggtacgcgc ttggcgggta ctttgatttt ctcaccgggt ttttgagggt
     1981 tgtttgtctc gcgggcttgt ctgtcgcggc gcaagaaggt gccgaagcca actagagtga
     2041 cgcgttcacc tttagctact acttcagtaa ctacatcggt gaaggcggcc agcatttctt
     2101 caacagactt cttggttgta ttggtacgtg cagcgatttc ggcagccagt tctgctttat
     2161 tcacaggggt tcctcctact caggtcctaa attcatgaga acaacatgct ttgtgtattc
     2221 ttggcggtct aataaccacg tgagaggcaa gaatacagca ccagcagggc tgaaacaaga
     2281 cacttggcat aaaaagtggt tcttgataat tcttcttttc tgcattcctt gtggcaagtg
     2341 cctcttgaag gcctcttctc gcccctcttt cttgtcactt ggaaattagc gaaagtggct
     2401 ccagagaaca atttttgtca gattgcccaa gttctcagaa agcgggaggg tagcaaagga
     2461 tttctttgga atataattca agaagggtaa ttaatgagct taaaagatac agagagggcg
     2521 tgagatcagc atggaagtgg gaacaaataa ggagtcggat ctcgctggtg tcctcttgag
     2581 ctcatccaca aacacacccg ttgtcgattc tcaggcggca gcctatcttg ctgccaatgc
     2641 ttgcgagagc aagaaagcta agtccaccac tgtccttgat gtcagtaagg tgaccctgct
     2701 tgccgattac tttgtcattg ccggtgccga gtctgccgcg caggtgcgag ccatcgtcga
     2761 cgccgttgac gatacactca ggcaggccgg tttcaaaccc aagtccatcg agggcaaaac
     2821 agaggcccgc tgggtgcttc tagacttcgg gcatgtgatt gttcacgtat tgcaagaaaa
     2881 agagcgcaat tactacaaaa ttgaacaatt ttggaatcat gccttgattg ttgataggga
     2941 agaatgggta caagagtaaa agcagaaacg tttttactct gactttcgga aaaggttatc
     3001 gagggccatt tgctatgagt ttcaggacca gattacaggg ctttgttgat tctagacaga
     3061 agattcaaat ctggtacggc atgggttgtc acaataccgt gaccgggcgg gttctcaccg
     3121 tccaccccga ccatatcgac gtagagtcct attcccatga aagtgacggt cagattcatg
     3181 tccgccgtat cctcattccc ctgcacctga ttctgcatat agacattacc tcagccgaaa
     3241 tttccgagga ggacgagacc aatttctcca ccgagaagga cggtaagtcc ggcgcgccga
     3301 ccaatgtcat agacctgccc gacgcccctc gcggttacgc ggtgagagta ctgtcccagc
     3361 ttaattctgc cttttccaat cgcttttcac ggatgactgc cggtcccggc ggtatttact
     3421 tctcctgcga cggcagtgtc tggttcatag acggaaacgg cgctctcagt gaatatgcgc
     3481 gtatccgcgg caacagcact atttccggcc tgcggtttga ccgcaagggt gtgctctacg
     3541 tggctaccat ccagggcacc gtct
//
LOCUS       DAOQNZ010000227         3560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12605_length_3560_cov_3.740086, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000227 DAOQNZ010000000
VERSION     DAOQNZ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12605_length_3560_cov_3.740086"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKW73_05360"
     CDS             <1..296
                     /locus_tag="PKW73_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72741.1"
                     /translation="QEALRHLSNNSQPCPLSAAGFTLRKTAFAPGIDDAISVFTASVK
                     VSLPLPSSTLIVLAPLERALEGNFEQQSPLIRYNRSYRILAGEFAEAEDAYPV"
     gene            280..1167
                     /locus_tag="PKW73_05365"
     CDS             280..1167
                     /locus_tag="PKW73_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72742.1"
                     /translation="MLTRSENTRNRQTAKFSSRSGNGGPCSLLPALFLSVVTAAPGSA
                     QNQIMNQPPPNLYAPAPPPTSPQTTSQAQPGLPALKGNQGARDNLDILYLGRGHYAGG
                     RLPEALAAFQEFARLKPNNLAAHFWLGTVYDELGRSREALDEYSQALRLAGSVGMDSP
                     ELRINLGNRLAREGYLKESLFDYQRSLVIDKRYALAYLGVAKCLLAQGDNQGALKALD
                     QYQQAGGNDVNAILLRGLTLASLGRGLEAQYHLRLFLKQAIPGLENTPGRASTAGGVN
                     TSNEAVELARKTLSTISDL"
     gene            1315..2541
                     /locus_tag="PKW73_05370"
     CDS             1315..2541
                     /locus_tag="PKW73_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72743.1"
                     /translation="MKKPELRIEADELGEMEVPMHSLWGVRTARWLSRSRAGAMQYQE
                     TRLHPLMVEALATIYKAQLAVARTVHSGLDGSDAAAIQAATTLKGQVLDEILNGQLKD
                     QLLVDLRLSPLGEAVFDNLNEVVEGRARELLGLKAHEGRQGAQIKSKPLATASGKAAS
                     RLPLTGASYFLAAVRLAANLALRDLEATLLDLERLVRRQSLTLEKSLRQMGRQSREEL
                     APATVFSLFGNAAERNLKRLKETRNKLLELALDAPQNYQYAFALELAALTGMPFREAE
                     QTNSPVEKEKSGDTLSRQAVMDLQQVAAMLKELSIDISHMCQVLSGLSESFTDSTVES
                     PDRLTSHSSSLSLACLRVQAEEMQVSLALQADINRSACPAISLAAHGILSSSDALKSA
                     LKSFNQGYLSRLKLRN"
     gene            2932..>3560
                     /locus_tag="PKW73_05375"
     CDS             2932..>3560
                     /locus_tag="PKW73_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72744.1"
                     /translation="MAFLADSSLETIAFMFSGYRFEMIVGCMSAGKSSELIRRIERAR
                     LAYLPTIILRPATDTRSKPNHVESRNGMTSQAIVVEDVKDVLKYSSHAVVIGLDECQF
                     FGDGVIDVIQTLLRQKKKIIASGLDLDYRGKPFGPVPTLLAMADRVDKLLAVCRKCGS
                     DFACRTQRLVHSSEQILVGDAQYEARCIHCFEPPGEYQLRLDLPKVEQAV"
BASE COUNT          869 a         1044 c          922 g          725 t
ORIGIN      
        1 atcaagaggc cctcaggcac ctcagcaaca attctcagcc ttgcccattg tcggccgccg
       61 gctttaccct cagaaaaaca gcctttgcac ccggtataga tgacgcaata tcagtcttta
      121 cagcctctgt aaaagtaagc ctgcccctgc cctcaagcac tttgattgtc ctggcgccct
      181 tggaaagagc cctggagggc aacttcgagc agcaaagtcc tttgatccgc tacaatcgca
      241 gctatagaat tttggcgggc gaatttgcag aggctgagga tgcttacccg gtctgaaaat
      301 acaagaaacc ggcagacggc taaattcagt tcccggtccg gaaacggcgg tccttgcagc
      361 ctgcttccgg cgcttttcct cagtgtcgtc acggcggcac caggttcggc ccagaatcaa
      421 atcatgaatc agccgccgcc caatctctat gctccggcac caccgccgac atcgccccaa
      481 acaacatcgc aagcccagcc ggggctgcct gctctgaaag gaaaccaggg cgcaagggat
      541 aatctggaca ttctttatct gggtcgcggt cactatgccg gcggcaggct accggaagcc
      601 ctggccgcct ttcaggaatt tgcccggctc aagcccaaca acctggccgc tcacttctgg
      661 ttaggcaccg tatatgatga attgggccgc tctcgcgaag ccctcgatga atactcccag
      721 gcgctccgtc tggccggttc tgtaggcatg gactccccgg aactgcgcat caatctgggc
      781 aaccgcctgg cccgggaagg ttatttgaaa gaatcactct tcgactacca gcgcagtctg
      841 gttatcgaca agcgctatgc cctggcttat ctgggtgtgg ctaaatgtct tctggctcaa
      901 ggcgacaatc aaggcgcgct caaagccctc gatcaatacc aacaggccgg cggcaacgac
      961 gtcaacgcca tacttctgcg agggttgacc ctggccagcc tgggccgagg actggaagca
     1021 caatatcact tacggctctt tctcaagcaa gccattcccg gtttggaaaa cacgccgggc
     1081 agagctagca ctgccggcgg tgtcaatacc agcaatgaag cggtggaact ggcgcgcaag
     1141 accttgagta cgatttccga cttgtaacgt ttcttcggcc agtgacctta agtgacggca
     1201 agggtcaacc acccttcctt aattgagctt gaagtaattt cccgggcaat gtgcataaaa
     1261 tagcccctca cagcaatttc aaccaagggc ccgagcccct caagaagaaa agccttgaaa
     1321 aaaccagaac tgcgtattga agcagacgaa ttaggcgaaa tggaagtacc gatgcattcc
     1381 ctctggggag tgcgcacggc gcgctggctg agtcgctccc gggccggcgc catgcaatat
     1441 caagagacca ggctacaccc cctcatggta gaagccctgg ccacaatata caaagcacag
     1501 ctggccgtgg cccgcaccgt ccatagcgga ttagacggct cagacgccgc cgcgattcaa
     1561 gcggcaacaa ctctgaaagg gcaagtactg gatgaaatct taaatggtca gcttaaagat
     1621 caactgctgg tagacctgcg cctctcgccc ctgggagaag ctgttttcga caacctcaat
     1681 gaagtcgttg aggggagagc cagagagtta ctcggactga aagcccatga aggccggcaa
     1741 ggagcgcaaa tcaaaagcaa gcctttggca acagccagcg gcaaagccgc gtcgcgcttg
     1801 cccctcaccg gcgcttccta tttcctggcg gcagtaagac tggcagccaa tctggccctg
     1861 cgcgatctgg aggccacttt gctcgatctg gagcggttag tgcgcagaca gtctctcacc
     1921 ctggagaaga gcctgcgcca gatggggcgc cagagccggg aagaattagc accagcaacc
     1981 gtcttcagcc ttttcggtaa tgccgccgag cgaaatctta aaagactgaa agaaacccgc
     2041 aacaaactcc tggagctggc cctggatgct ccccagaact atcagtacgc cttcgctctg
     2101 gaactggcgg ctctgaccgg catgcctttc cgggaggccg agcagacaaa ctcgccggta
     2161 gagaaagaga aaagcggcga caccctctcg cgccaggcgg tgatggattt gcagcaagtg
     2221 gcggccatgc tgaaagaact atctatagat atctctcaca tgtgccaggt tttgagcgga
     2281 ctgagcgaaa gcttcaccga cagcactgtt gaaagcccgg accggcttac ttcccattcc
     2341 agcagcctca gtctggcctg cttgagagtg caagctgagg aaatgcaagt cagcctggcc
     2401 ctgcaggccg acataaatcg cagcgcctgt ccggccataa gcctggcggc ccacggcatc
     2461 ttaagcagca gcgacgccct caaaagcgcc cttaagagtt tcaatcaagg ctatctgagt
     2521 cgcctcaaac taagaaacta gatgagaatt tgaacaggcg cagttagtaa tttgcccgcg
     2581 ccactttaca agccggttta catttaaatt tgccccgggg cgctgcctgt agtaccgccg
     2641 gtagtatcag ccaccaaagc cggcaagacc ccgaggttgt cgtccaatat attttatctt
     2701 ggcgccggtc cctgcatttc tgcccttttc cagcgtagcg aatatcgaca aaatatcgaa
     2761 accataacgc cagacaataa caagtaatac aaaaaggtcc gagagcccgg caattatgaa
     2821 agcgatttga tctggcaaat cgttcaaatt tggttaccat tagtacccgc atggcatctt
     2881 gctcactgaa accggcccat gcatctatat aagacgggtg agcagcgcta tttggcgttt
     2941 ttggcagatt cttccctgga gacaatagcc tttatgttca gtggttaccg tttcgaaatg
     3001 attgtcggct gtatgagcgc cggcaaatcc agcgaactga tcagacgaat cgaaagagct
     3061 cggttggctt atctaccgac catcatcctg agaccggcca ccgatacgcg ttccaagccc
     3121 aatcatgtgg aatcgcgcaa cggcatgacc tcccaggcca tagtcgtgga agacgtcaaa
     3181 gatgtgctca agtattcctc ccacgccgtg gtaatcggtc tcgatgaatg ccagttcttc
     3241 ggcgacggcg ttatagacgt aattcaaacc ctgctgcgcc agaagaagaa aatcattgcc
     3301 tccggtcttg acctcgacta ccgcggcaag cctttcggcc cggtaccaac ccttctagcc
     3361 atggccgacc gcgtcgacaa gctcctggca gtctgccgca agtgtggttc cgacttcgcc
     3421 tgccgcaccc agcgcctggt gcactcaagt gaacaaattc tggtcggcga cgcccaatac
     3481 gaagcccgct gcattcactg ttttgaaccg ccgggtgagt accagctgcg gctggatttg
     3541 cctaaagtag agcaggcagt
//
LOCUS       DAOQNZ010000228         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12613_length_3558_cov_4.068227, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000228 DAOQNZ010000000
VERSION     DAOQNZ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12613_length_3558_cov_4.068227"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKW73_05380"
     CDS             complement(<1..916)
                     /locus_tag="PKW73_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA72745.1"
                     /translation="MRIKEIELDNFKSFGKQTLVPLLDGFTTISGPNGSGKSNIIDSL
                     LFALGLSSTRTMRAERLPDLLNNLSGRNEARVRVKFTNDEGHELEVIRRIRVKDTGYT
                     STYILNGKVATLTEVHDELIKYNVSPTGYNVIMQGDVTGIVTMSAGERRKIIDELAGV
                     AEFDRRIDQAQNELSSVGEKIELQKIILTEILARLEILKTDRDQALKYLDLKTQKETV
                     ERDLVFVRAAELEEKASLELKEIEKLNSKEEALNEKLDKTEVALVSLRAELGRIEQEI
                     REKGGNEQLLLRQDLENKRGELTREENKL"
     gene            1325..2512
                     /locus_tag="PKW73_05385"
     CDS             1325..2512
                     /locus_tag="PKW73_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72746.1"
                     /translation="MSQHTSSQIGDLLSGAGLVQRENIEAALSMSATRRQPLGKILID
                     SGLMTEMELYGTLQAQNLIREHFLQPDLALTMLSRIRETGRSFLENLIESGANIEVID
                     FGLSLGELFIDAGAINRADLMTAMETSIVSGLPLVRVLILQNSLDEKSAYSGLTAKLL
                     VKEGRIARGEAVGALKLSHMHGDHIEEILEFGGFKKFREQNFVRLGELLVLSELISEL
                     DLLGCVEKSMSAAQPLGQVLVDEGILAEQLVQSALRAQKYIEAGTVDALRASQMLRHC
                     ARTGQLLEFSLEELASIKPKVLMLEKPQSLAALLEILGLVPLSELEQIELVTIQESDE
                     EKVKEFILNRQILTRECLEAVLYGYELVKSGRIATEQLVFAVHVWLWNRGDFRALITT
                     LGW"
     gene            complement(2493..>3558)
                     /locus_tag="PKW73_05390"
     CDS             complement(2493..>3558)
                     /locus_tag="PKW73_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72747.1"
                     /translation="LIAAGQTLESSPRHLLGDPVVLLGSSLVMSPLWAADVRLGHFLQ
                     DVNMHHRSLHMKEEMDKFGYHKDAVSLATPGQFVSDAYLIVDKHLKDKNRPEILIYGI
                     APRDFMDSTTSGLVTSVFNQLVAITDMPKIKDLFLESFDDHMNFVLERTVFLYHKRTR
                     YQTKFEEAVEKLTCRLDKYLAPKTESAAAPFLMGTDHDAIWKQSTEEYARRYKTANEP
                     LFQKQTKCLRALMQLCKERNIKLYIVAMPLSKTNMALMPPGLYQHYMDTLKTETGKAD
                     VPLIDLAADGNYGDDDFYDTVHLNQTGGERFIKQMTALVRANSQGIAFTPELLKNVNV
                     ESPEPVKPQLATPALTSPAL"
BASE COUNT          796 a          873 c          957 g          932 t
ORIGIN      
        1 agagcttgtt ctcttccctg gtcaattcgc ctcgtttgtt ttcaaggtcc tgccgcaaga
       61 ggagctgctc gttgccgcct ttctcgcgaa tctcttgctc aatgcgaccg agttcagccc
      121 tcaggctgac cagagcaact tcggttttgt ccagtttttc gttgagggcc tcttctttgc
      181 tgttgagctt ttcaatttct ttcagctcta agcttgcttt ctcttccaat tcggcggcgc
      241 gcacgaagac cagatcgcgc tccacggttt ctttctgggt cttgaggtcg agatacttga
      301 gggcctggtc gcgatcggtt ttgagaattt caagccgtgc caggatctca gtgagaataa
      361 ttttctgcag ttcaatcttc tcgcccacgg aagaaagttc gttctgggcc tgatcgattc
      421 ggcggtcgaa ttctgctacc cccgccaact catcgatgat cttgcgcctt tccccggcac
      481 tcatagtgac gatgcccgtg acgtcgccct gcatgatcac gttgtagccg gtggggctaa
      541 cgttgtactt aattagctcg tcgtgtactt ctgtaagagt ggcaacctta ccattgagaa
      601 tataagttga ggtatagcca gtatccttga cgcgtatgcg gcggataact tccagctcgt
      661 ggccttcatc gttggtgaac ttgactctca ccctcgcttc gttgcgaccg ctcaggttgt
      721 tgagcaagtc aggaagccgc tcagctctca tggtcctggt agaggacaga ccaagggcga
      781 agagcaaact atcaatgata tttgatttgc ctgacccgtt cggaccggat atggtcgtga
      841 atccgtctag aagcgggacc agagtctgct tgccgaaaga cttgaaatta tcaagctcga
      901 tttctttgat tcgcaaaaga gactcccaga gttggaaaac gctattggct cgtcagtgta
      961 cagccgaatt agactacaca ctgcatttgg tgtcaagcat agaaccctat tttagctcgc
     1021 caggggcagt atgtcatcca tttggggctg gggcggtgag gaagaattgc atgattgggc
     1081 tctgtatata ggctgcttat gcttggtcta tctgatttac cgtgcctttt ctttagtatc
     1141 accggcggta taagtatata ctatggtaag taacatgatc gtgctacttt ggcatgtacc
     1201 ttcaatttcc acctcctgaa aggtcccgaa ccgtaggcaa aattcctcat ctcctgcagg
     1261 aattttgccg ctctttttat atcaagagga tatactttag cagtagcatt ttagaggtaa
     1321 acccttgtca cagcatactt ccagtcagat aggcgatctc ttgagcggcg ccggactggt
     1381 gcaaagagag aacattgaag cggctttgag tatgtccgcg acacggcggc aaccccttgg
     1441 aaagatttta atcgactccg gactgatgac ggaaatggag ctctacggta ccctgcaggc
     1501 tcaaaacctg atcagggaac acttcttgca gccggacctg gccctgacca tgctgtcccg
     1561 catacgggaa accggccggt ccttcctgga gaatttaatt gaaagcggtg caaacattga
     1621 agtgattgat tttggtcttt cgctgggaga gctctttata gatgccggcg ccatcaatcg
     1681 cgccgatttg atgacggcta tggagaccag catcgtctcg ggcctgcccc tggtgagagt
     1741 actaattttg cagaattccc tggatgagaa gtcggcttat tcggggctca cggctaagct
     1801 cctggttaag gaggggcgca ttgctcgcgg cgaagccgtc ggcgccctca agctctcaca
     1861 tatgcacggt gatcacatag aggaaattct ggaattcggc ggctttaaga agtttcgcga
     1921 gcagaatttt gtcagacttg gcgaattgct cgtgctctcg gagctcatca gtgaacttga
     1981 tctgctcggc tgtgtggaaa agagtatgag cgcagcccag cctctggggc aggtgctggt
     2041 ggacgagggc atactcgcgg agcaactggt tcagtccgcc ctgcgtgccc aaaagtatat
     2101 tgaggccggt accgtggatg ccttgcgggc cagtcagatg ctacggcact gtgcccgcac
     2161 cggccagctt ctggagttca gcttggagga attggcttcc atcaaaccca aggtcttgat
     2221 gctggaaaaa ccgcagtcgc tggcagccct cttagagata ctgggactgg tgccactttc
     2281 ggagcttgag caaatcgagc ttgttaccat tcaggaaagc gatgaagaga aagtgaaaga
     2341 gttcatcctg aacaggcaaa tattgacgag agaatgcctg gaggctgtac tctacggcta
     2401 tgaactggtg aagagcgggc gcattgccac cgagcaactg gttttcgccg tacatgtctg
     2461 gctctggaat cgaggcgact tccgggcact gattacaacg ctgggctggt gagggccggc
     2521 gtggccagct gaggtttgac cggttccggg ctttctacat ttacgttttt cagaagttcg
     2581 ggagtgaagg caatgccctg agagttggct ctcacgaggg ccgtcatttg tttgataaag
     2641 cgctccccgc cggtttggtt caagtgcacg gtgtcataaa agtcatcgtc gccatagtta
     2701 ccgtcggcgg ccaggtcaat caaaggtaca tcggccttgc ccgtttctgt tttgagagtg
     2761 tccatgtaat gctggtagag cccaggcggc atcaaagcca tattcgtctt gctcaatggc
     2821 atagccacta tatatagttt gatattgcgt tctttacaga gttgcatcag tgccctcaag
     2881 cacttggtct gtttctggaa aagcggctca ttggctgttt tatagcgtct ggcgtattcc
     2941 tcggtactct gtttccagat ggcatcgtgg tcggtgccca tcaaaaatgg cgcagcggcg
     3001 ctttcagttt tgggcgctaa atacttatcg aggcggcaag taagtttctc tactgcttcc
     3061 tcaaacttag tctggtagcg ggttcgctta tggtaaagaa agaccgtgcg ctccagcacg
     3121 aaattcatat ggtcgtcgaa gctctccaga aaaagatctt tgatcttcgg catatcggtg
     3181 attgctacca gttgattgaa gaccgaagtg accagaccgg aagtagtact atccataaaa
     3241 tcccggggcg caattccata tataaggatt tccggacgat ttttatcctt gaggtgcttg
     3301 tcaactatca ggtaggcatc ggaaacgaac tggccgggcg tggccagaga gacggcatcc
     3361 ttgtggtagc caaatttatc catctcttcc ttcatgtgca ggctgcgatg gtgcatattc
     3421 acatcctgca agaaatgccc caggcgcaca tcggcagccc agagcggcga cattacaaga
     3481 gatgaaccca gcaaaacgac gggatcgccc agaagatgac ggggtgatga ttcaagcgtc
     3541 tgaccggcag caatcaag
//
LOCUS       DAOQNZ010000229         3549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12679_length_3549_cov_3.783629, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000229 DAOQNZ010000000
VERSION     DAOQNZ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12679_length_3549_cov_3.783629"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2632
                     /locus_tag="PKW73_05395"
     CDS             <1..2632
                     /locus_tag="PKW73_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72748.1"
                     /translation="HYICAAGAAEAPYVKQLERGLRKLKKGDYEDAVSCFTSLLKDYP
                     TVYEAYLKRAGARAELKDLDGALKDYEQAIKTNPNATESYLERGALYLKLGDRAAAIA
                     DYSEALRLSPRDGKARLLRAQVLHKNGDYAGAVQDLDQVLKVEPGNVVARKERADCKS
                     HLELYDEAIADYEYLLKKNGSKVFPVHYELGEALQLKGDKKAGSEHFNLVVQHYTREL
                     SRSSKKGGDYIYRGMAYYQLGEKEKALRDLESGVALLTDDAQANYQLGHLKYRLGDNE
                     GALKVLDRALKLDPKLHPALVDRAEIYSSQGDFGAALTDLNQALALSRSPEELSDRAM
                     VRLSIGDVGGGVQDILEASKLSPTFIGRKQKEVAEKLEAASGKPESARLQAGTLLQIA
                     LLDLACGNIDSAESTARQAISILDKLTREKKLSASDPQNAYALLLLGNVYLKKRSLVE
                     AESLFRIALNKLRNTASGEQKYAVFSLEECAKMLIAASSMEEAGSILSDTRLVRATTG
                     LTENAFSSDMSRKAEQAVEAYKQKRKYDRQEDLVRRAASGSASAGEEGTGGVEEQIQI
                     NRPIRDKWALIVGTGRFKDSRINLKYAAKDAEDFANFLIKEKEFAPDHIQLLTNEAAT
                     RANILSLLGSKWLPRVAERDDLVVIYFSSHGSPSSLDVGGVNYLVAHDTDPNDLYATG
                     IAMQDLARIIKGRVHCDRIMLVLDACHSGSAGPSTAAGKGMERAANVNVEQVMQGTGQ
                     LVISSSKPDQRSWESSRYEGSVFTKYLIEGLRRNGKLTKLGDAYAYLESEVQREVLRD
                     RGVLQTPVMRSKWRGTDLIIGVTPAQPSPGLGDIELPDAVDSKLAKGSGKSATISGGS
                     ASAGVGHSAGGRGRTRGKRR"
     gene            2696..>3549
                     /locus_tag="PKW73_05400"
     CDS             2696..>3549
                     /locus_tag="PKW73_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72749.1"
                     /translation="MNTSKKQSEGRKAGGSGIPRLKSVLIALTLGLLLAPPVSCQQMI
                     WQAFNDSGSKFYDEHDYERAEKSLLNAVKEAEKLSKDGPELVASLKLLRKVYLALDKQ
                     ALAAQVAERLSTLGASESAGETSTGTSGEVSTRSENAAPKADTASRPADNPSSARTEA
                     ASKSENADVAPALDGSVDSGDKTLRRDGGSAAPSNSASSSAPATSVAGSGTASAPVIA
                     SSRGIDGELRVGQGVKKAVELLKLSGHISWEKSVAISSDGLRAISGSDDDTVRLWDLQ
                     TGKELKRF"
BASE COUNT          814 a          922 c         1045 g          768 t
ORIGIN      
        1 ccattatatc tgcgcggccg gcgcggctga ggctccttat gtcaagcagt tggagcgcgg
       61 tctgagaaag ctcaagaagg gcgactacga agatgccgtg agctgtttta ccagtctatt
      121 gaaggactat ccgactgttt atgaagctta tttgaagcgg gccggcgccc gggcggagct
      181 gaaagacttg gacggcgccc tgaaagatta tgagcaggcc ataaagacca atcccaatgc
      241 cactgagtct tacctggaaa gaggcgctct gtacctgaaa ttgggtgatc gagcggctgc
      301 cattgccgac tattcggagg cgctgcgcct cagcccccgg gatggtaagg ctcgtcttct
      361 aagggcgcag gttcttcaca aaaacggcga ttatgccggg gcagttcaag acttagacca
      421 ggttttgaaa gtggagccgg gcaatgtggt ggcccgcaaa gagcgggcgg attgtaagtc
      481 gcacctggag ttatacgacg aggccatcgc cgactatgaa tatctcctca agaaaaatgg
      541 ttccaaagtc tttcccgtgc actatgagct gggagaggcg ctgcaactga aaggtgacaa
      601 aaaagccggc agcgagcact ttaatctggt ggtgcagcat tacacccgtg agctctctcg
      661 ttcttccaag aagggcggtg attacattta tcggggcatg gcctattacc agttgggcga
      721 aaaggaaaaa gctttgcgtg acctggaaag cggcgtggcg cttctgaccg acgacgccca
      781 ggccaattac cagttggggc atctaaagta ccgcctggga gacaatgaag gggccttgaa
      841 ggttctggac cgggccctca agctcgatcc caagttgcac ccagcccttg tggacagggc
      901 cgagatctat agcagccagg gtgatttcgg tgcggccctc accgatttaa atcaggctct
      961 ggctctttct cgcagcccgg aagagctctc tgatcgggct atggtaaggc tctccatcgg
     1021 tgacgtcggt ggcggtgtac aggatatttt ggaagccagt aaacttagcc ccactttcat
     1081 cgggagaaag cagaaagagg tggcggaaaa actggaagca gcctcaggca aacccgaatc
     1141 ggctagattg caggcaggca cgctgttgca gattgccctg ctcgatctcg cttgcggcaa
     1201 tatcgattcg gctgagagca cggccaggca ggcgatttct attcttgata aactaaccag
     1261 ggagaaaaag ctttccgcct ctgacccgca gaatgcctat gcgctcctgc ttttgggtaa
     1321 tgtgtatctg aagaagcgct ctctggttga agctgagtcg ctttttcgca tcgccctcaa
     1381 caagctgcgt aataccgcca gtggtgagca aaaatatgcc gtgttttccc tggaagagtg
     1441 tgccaaaatg ctcatcgccg cctccagtat ggaagaagcc ggttcgattt tgtcggatac
     1501 acgcctggtg cgggctacaa ccggtttaac cgagaatgct ttttcttccg acatgtcgcg
     1561 caaagcggag caggcggtgg aagcctataa acaaaagcga aaatatgatc gacaggaaga
     1621 tctggtgcgc cgggctgctt caggcagtgc atccgccggg gaagaaggta cgggcggcgt
     1681 agaggaacag attcagatta accggcccat tcgcgataaa tgggctttga tagtcggtac
     1741 agggcgcttc aaagattctc gtatcaatct caagtatgcg gccaaggatg ctgaagactt
     1801 cgccaatttc ctcatcaagg agaaagaatt cgctcccgac catattcagc ttctcaccaa
     1861 cgaagctgct acccgggcca acattctttc gcttctgggc agcaagtggt tgccgcgggt
     1921 ggccgagcgc gacgatctgg tagtaattta tttctccagt catggcagcc cctccagtct
     1981 tgatgtgggc ggcgttaatt atcttgtcgc tcatgatacc gatccaaatg acctctacgc
     2041 caccggcatc gccatgcaag atctggcccg catcatcaaa gggcgggtgc attgcgatcg
     2101 catcatgctt gtgcttgatg cctgtcacag cggttcggcc ggcccctcca cggctgccgg
     2161 caaaggtatg gagcgtgctg ctaacgtcaa tgtggaacag gtgatgcagg gcaccgggca
     2221 actggtgatt tcctccagta agcccgacca gagatcctgg gaaagcagtc gctatgaggg
     2281 cagcgtcttt accaagtacc tgatagaggg attgcgtcgc aacggtaagc tgaccaaact
     2341 tggtgatgcc tatgcttatt tagagtcgga agtgcagcgg gaggtgctgc gagatcgcgg
     2401 cgtcctgcag acgcctgtca tgcgcagcaa atggcgcggt accgatttga tcatcggcgt
     2461 gacgccggct cagccttccc ccggtttggg tgatatagaa ctgcccgatg cggtggattc
     2521 taagttggca aaaggcagcg gaaagtctgc taccataagc ggcggcagtg cttctgccgg
     2581 ggtgggccac agtgccggtg gtcgcggccg cacccggggc aagcgccgct gacggcattc
     2641 aagaaagtct ttctgaaagc catctctttg cactaaaacc agtatgggaa agctaatgaa
     2701 cacttccaag aagcagtccg aaggcagaaa ggctggaggc tccggcatac cacggcttaa
     2761 gagcgttttg attgccctca ctcttggcct gctcctggcg ccgcctgttt cttgccagca
     2821 gatgatctgg caggctttca atgatagcgg cagcaagttc tacgatgaac acgattatga
     2881 acgggccgag aagagcctgc tcaacgccgt taaggaagct gagaaattga gcaaggacgg
     2941 accggagctg gtggccagct tgaaactcct gcgcaaagtc tatctggctc tcgataagca
     3001 ggctctggct gctcaagtgg cggagcgact ttccactctg ggcgcctctg aaagtgctgg
     3061 cgagacctcc actggtacaa gcggcgaagt gagtacaagg tctgagaatg cggcgcctaa
     3121 ggctgatact gcctccagac cagccgataa cccttcttct gcccggacgg aagctgcatc
     3181 caagtctgaa aatgccgatg tcgctcctgc tctcgatggt tccgttgact ccggggacaa
     3241 gaccctgcgc cgagacggcg gcagtgctgc cccgtcaaat agtgcttcca gcagtgcccc
     3301 tgctacttct gttgccggta gcggcactgc ttctgcgccc gtgattgcct ccagtcgagg
     3361 tatagacggt gaactacggg ttggtcaggg agtgaaaaag gccgttgagc ttttgaaact
     3421 tagcggtcat atcagttggg agaaatccgt ggccatctcc agtgacggtc tccgggctat
     3481 ttccggcagc gatgacgaca cggtgcggct ctgggattta cagaccggca aagagttgaa
     3541 acgcttcga
//
LOCUS       DAOQNZ010000230         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12691_length_3547_cov_3.783219, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000230 DAOQNZ010000000
VERSION     DAOQNZ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12691_length_3547_cov_3.783219"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="PKW73_05405"
     CDS             <1..1332
                     /locus_tag="PKW73_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNA72750.1"
                     /translation="ALTATATPKVQKDIITNLGLNMEVVKGDFDRPNLILEVEKCDNP
                     ADKDRHLFARLKHDPAGHGGSAIVYVSSRKEAEALSKRLNGEGIKAAFYHAGLPAPMR
                     KATQKRFEDDLVKVIVCTVAFGMGVDKANIRKVVHYNLPPSLENYYQEAGRAGRDGQP
                     ATCTLLYQPKDIYTQRWLTKMNYPTEKQIFEVYEFLRLSQGSPVRPSAILEAVPDIKE
                     TPLHSTLDLFNQLGLLTLSPGGITLKTIPSYLDTSILTRRKEQDEDRLERMISYALTP
                     LCRRRTILGYFGQALVNDCSGCDVCHPHLRMTFSPTPSRAHRAVNPERETIKKARQAL
                     AARQFDQDGFISAADAANIKSQAGKGDNLSQAIVSLTTELKGQVGRTTVASILAGSKA
                     KKLADKGFDKLSLYGACAHRNQDTILEAIDALIEEGHLRVIPGMYPKLTIA"
     gene            1724..1990
                     /locus_tag="PKW73_05410"
     CDS             1724..1990
                     /locus_tag="PKW73_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72751.1"
                     /translation="MPEDQNRNNEPKLPSVLYMWTPVLAFVVIGIYSALDFQARTNLL
                     DAHIKTVETVAAFFWAGILGALMTAFALLFVELTVRDTFRLLHK"
     gene            2121..2756
                     /locus_tag="PKW73_05415"
     CDS             2121..2756
                     /locus_tag="PKW73_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72752.1"
                     /translation="MASQESDSAKMARLAYEEMERKFQDLLSPENQERARVHLEGARQ
                     SLNSLAQKGKEGLQLAIDLVSELTRELTEVEEFNVKPKSKSESTQNAELEIDIKIKKE
                     KELPIKTVVAPLVEMEAVIVGKRIHFQSIIDKSKNGLRCNFNEGFALRLKTPLGKHTI
                     AIKGTAILRRDENKQIVLETPLTIPGTQTEVSVSIPIKQLLHEAGKKSWLG"
     gene            2744..>3547
                     /locus_tag="PKW73_05420"
     CDS             2744..>3547
                     /locus_tag="PKW73_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72753.1"
                     /translation="MAGLRIALLASVVVLTLTSCTTGGAPLELSVDGETVSQENSGSR
                     DRFKPRTEAADSLWLADGAVETFPYLPETDEHEHLHELAEKQLNADPFAADGHRAVAW
                     IYYNETNFKEMADCAARAVVLSPASKINRLCLSLAMYGRGAHQQAFEMASQALVLPPS
                     VNVPQNDCRLYLCRAASALNANRYQASLADADLALKISPDHIYANLLRGEALTGLERN
                     QEALTCFRKSLNYINTKDTAPYTLLKAETLLARAKAYLLLGEKDAARADL"
BASE COUNT          968 a         1030 c          848 g          701 t
ORIGIN      
        1 gctctgacag ccactgccac acccaaggtg cagaaggaca tcatcaccaa ccttggtctc
       61 aatatggaag tggtgaaagg tgacttcgat cgccctaact taattttgga agtggaaaaa
      121 tgcgacaatc cggccgacaa agacaggcat ctcttcgccc gactgaagca tgatccggcc
      181 ggtcatggcg gcagcgccat cgtctacgtc agcagccgca aagaagcaga agcactctct
      241 aagcgcctca acggcgaagg catcaaagcc gccttttacc atgccggctt accggcaccg
      301 atgcgcaaag ctacccagaa gcgttttgaa gatgatctag tgaaagtgat tgtctgcaca
      361 gtggccttcg gcatgggcgt agacaaagcc aatattcgca aagtagtgca ctacaatttg
      421 cccccttccc tggaaaacta ctatcaggaa gccggcaggg ccgggcgcga cggacaacca
      481 gccacctgca ccttacttta ccaacccaag gacatctaca cccagcgctg gctgacgaag
      541 atgaactatc ctaccgaaaa gcaaatcttc gaagtctacg aatttctgcg cctcagccag
      601 ggtagcccgg tcagaccatc cgccatactg gaggcggtgc ccgacatcaa agaaacaccg
      661 ctgcactcca ccctcgatct cttcaaccag ttgggcttgc taaccctcag cccgggcggc
      721 attacactca aaaccattcc ttcctacttg gatacatcca tccttacccg tcgcaaggaa
      781 caagacgaag acaggctaga gcgcatgatt tcctacgccc tgacgccgct ctgccgaagg
      841 cgcaccatcc tgggatattt cggacaggca ctggtaaacg actgcagcgg ctgcgatgtc
      901 tgccacccgc atttgagaat gaccttcagc cccaccccca gccgtgccca ccgggccgtc
      961 aacccggaaa gagaaaccat caagaaagcc agacaggccc ttgctgccag acagttcgac
     1021 caggatggtt tcataagcgc ggccgatgcc gccaatatca aaagtcaggc cggcaaaggc
     1081 gataatctca gccaggccat agtcagcctc accacagaat tgaaaggtca ggtaggacgc
     1141 accacggtag cctcaattct ggctggaagc aaagccaaga aacttgccga caagggcttc
     1201 gacaaactct cgctctatgg cgcctgcgcc catcgcaatc aggacacgat cttggaagcc
     1261 atcgatgcac tcatcgagga agggcatcta cgcgtcatcc ccggcatgta ccccaagctg
     1321 accattgcct gagagcgctt tcccgaccag agacttaagc ctcaggctaa ttgtcccggc
     1381 aatggagcct taagcctcag gctaattgtc ccggcaatgg agccttacaa acacaattga
     1441 gccgtaaggc aattaagcca aaagtatgat tactgacagg aagcaaacct tcccaatggt
     1501 aaatattgac aaccagggct gcttcacgct ttcgagccgg agccaggcgc ggaccggtga
     1561 ccggctgcct aagcccttcg gcaaggccta agcggcaact ggtatacaaa aatactccac
     1621 cttgagatgg attacgcctt cgggaagccg tagatatcat cgaaatatcg tttccaatta
     1681 cgacgctact ttgtccctta cttccaaccg gatggtggat actatgcccg aagaccaaaa
     1741 ccgaaacaat gagccgaagc tcccttccgt cctctatatg tggactccag tgctggcatt
     1801 tgtggtaatc ggcatctatt ccgccctgga ctttcaagct cgaaccaacc tgcttgatgc
     1861 ccatatcaag accgtagaaa cagtagcggc gtttttctgg gccggtatcc tgggagcgct
     1921 catgaccgcc ttcgctctac tgtttgtaga actgacggtt cgcgatacct tccgactcct
     1981 ccacaagtaa atcaccagta gaccaccagt aaatcacaag tagatcttaa gcacgctaaa
     2041 ctcctcaccg ccgccttgtt tgcaaggtgg cggtgttatc attagagaac caaacagtct
     2101 caggttccgg agaatagaaa ttggcgtctc aagaaagcga ttcggcaaaa atggccaggc
     2161 tggcctatga agaaatggaa cgtaagtttc aggacttact gagtccggaa aaccaggaac
     2221 gcgcaagagt tcatttggaa ggcgcccgtc aatctttaaa cagtcttgcc cagaagggca
     2281 aagaaggact gcaactagca atagatctgg tcagcgaact gacccgcgaa ctcaccgaag
     2341 tggaagagtt caacgtcaaa cccaaatcca aatccgaatc cacacaaaac gccgaacttg
     2401 aaatcgacat caagatcaaa aaagaaaagg aacttcccat caaaaccgtg gtggcaccgc
     2461 tggtagaaat ggaagccgta attgttggaa aacgcattca ctttcaatca atcatagaca
     2521 agagtaagaa cggactgcgt tgcaatttca atgagggctt cgccctgcgt ctgaaaaccc
     2581 cgctcggcaa gcacactatt gccatcaaag gcactgccat tctccggcga gacgaaaaca
     2641 aacagattgt attggaaact ccactgacaa ttcccggcac tcagacggaa gtttcggtca
     2701 gtatcccgat caagcaactc ttgcatgaag ccggcaagaa atcatggctg ggctgaggat
     2761 cgcactgctg gcctcagtgg tggtgctcac ccttacttct tgcactaccg gcggtgcgcc
     2821 tctcgaacta agtgtcgacg gtgaaacagt ctcgcaggaa aactccggca gtcgcgacag
     2881 gttcaaaccc agaacggaag cggcagactc actctggctc gccgacggtg cagtagagac
     2941 ctttccctac ctaccggaaa ccgatgaaca cgaacacttg catgaactcg ccgaaaagca
     3001 attgaacgca gatccctttg ccgccgatgg gcaccgcgcc gtggcctgga tttactacaa
     3061 cgaaaccaat tttaaagaga tggccgactg tgcggcccgg gctgttgttc taagtcccgc
     3121 cagcaaaatc aaccgcctct gcctgagcct ggccatgtat gggagaggtg ctcaccaaca
     3181 ggcttttgaa atggcttcgc aggctctggt cttgcctcct tccgtcaatg tacctcagaa
     3241 cgactgtcgc ctctacctgt gccgggccgc cagtgcactc aatgccaata gataccaggc
     3301 aagtctggca gatgccgatc tggctctcaa aatatcacct gatcatattt acgccaatct
     3361 cttgcgaggc gaggccctga ccggtcttga gcgcaaccaa gaagcactga cctgcttccg
     3421 gaagagtctc aactatataa atactaaaga cacagcgcct tacactttac tgaaggcgga
     3481 aactttgcta gcacgagcca aagcgtactt gctgctaggt gagaaggatg ccgcaagagc
     3541 cgacctg
//
LOCUS       DAOQNZ010000231         3546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12697_length_3546_cov_4.321398, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000231 DAOQNZ010000000
VERSION     DAOQNZ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12697_length_3546_cov_4.321398"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PKW73_05425"
     CDS             complement(<1..1097)
                     /locus_tag="PKW73_05425"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNA72754.1"
                     /translation="MFFVWAGAALADEIVSLDTEVEAQESATCHVRETISVKLDNGSR
                     YFERRIPIAYTGLSQDGVKDASLYHLGFRLIKVSQGQGRPVYFQLKQGAKEVVLRIST
                     RGSGRQGSDTMAEFKVEYLLRRAFTFDKENSQLKLSLTGPDCPYNANYVSMRVTFPRA
                     LQKEAGKLKVFLLDGREQIVASEKQIGISGMRLDKYNNLAMVSVANLARQTPVEAVFQ
                     LPAGIVERPGFLRLAGQFMVDWYPLFLLPALTIFALLSFRFNYKVQPLPGLVNPEAAA
                     TNPEAAATHPETEWGNSEKLPSPVIVSAVLTGHLSGKAEQDSLPLAALELACRGYLKI
                     LVTEGEGGERRLRFHKGACDPQYQLLTGFER"
     gene            1299..1751
                     /locus_tag="PKW73_05430"
     CDS             1299..1751
                     /locus_tag="PKW73_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNA72755.1"
                     /translation="MTVDCLICRKAGDEENRIHYLPHGLYLYESKYFRVRHSDETKIA
                     GYLIIESKRHFLDLSEATEEEAAEFGVELARVVRAVRRVTHPKRVYTFSLAEAVPHFH
                     VHVIPRDENLPARYVGRGILSYPLQPSLNESTRDEVSQALRRVFKALS"
     gene            1878..3125
                     /locus_tag="PKW73_05435"
     CDS             1878..3125
                     /locus_tag="PKW73_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72756.1"
                     /translation="MSAESEKMNLGSENVAMLGELFYRSGLVSIDQLCHALYVAHKNS
                     LPLGRVLVMLGMTKTRILEGALTLQNLVRQNLLTPALAVHCLQILGTNKVRVEEVLSE
                     MGWEGNPHKDEGNVGEILVEAKILTRKSIDDALGLSRHIGLPLPRILYLQGMVSSKLL
                     NATMKCQDVIRSGRVQKEEAIQALASVASMLEFEKACENALETKQPFIKLEELLLLAR
                     LLSQRDLQNFEEQMRIVGVKNLGQYIVECGFFSSTLIASAVRLQSMIARFELTPLSAA
                     GALRLIFSRNLSLADALEEIGKERPPYDGEISLYHLLRLSGLVTAFDVRRLGHLPGTA
                     GPQLTDPIEERLFKAGILSKASIDAAKRCQLLMREGFLTAEQALIVLQHWSWSGEGLT
                     RVLEKLHWGPGNMPTLHAQAFRS"
     gene            complement(3126..>3546)
                     /locus_tag="PKW73_05440"
     CDS             complement(3126..>3546)
                     /locus_tag="PKW73_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72757.1"
                     /translation="VQQLRAKYAPKLQQLEEKVRLAQQKLEAEAAEAKRQDMESALSI
                     GATVLGAFVGRKMGLGRAGTAARGVNRAAKQREDVERAKENLQVLHEKLERLNAEFAS
                     EAALAQSGSGAEISVREVCAKKSNISLQLLGVGWVRG"
BASE COUNT          873 a         1019 c          880 g          774 t
ORIGIN      
        1 cttcgttcaa atccggtaag caactggtac tgggggtcac aggcaccttt gtggaagcgc
       61 agccggcgct cgccgccttc cccttccgta accaaaattt tcaaatagcc acggcaggcc
      121 agctcaaggg cggccagagg cagactgtct tgctcagcct tgcctgacaa atgcccggtc
      181 aagaccgccg aaactataac cggcgagggc agcttctcgg aatttcccca ctcggtctca
      241 gggtgtgtcg ccgctgcctc agggtttgtg gccgctgcct caggattcac aagcccgggc
      301 aaaggttgca ccttgtaatt gaaacgaaag ctcagaaggg caaaaattgt cagggccggc
      361 agcaggaaga gcggatacca atccaccatg aactgccccg ccagacgcag gaagccggga
      421 cgctccacaa taccggcggg aagctgaaag accgcctcca caggagtctg tctcgccagg
      481 ttggcaacgc ttaccatggc taaattgttg tacttatcca ggcgcatgcc ggagattcca
      541 atctgcttct cactggctac gatctgctca cggccgtcca gcaagaagac cttcaacttg
      601 ccggcttcct tttgcagtgc ccgggggaag gtaacgcgca tggaaacata gttggcgtta
      661 taagggcaat caggccctgt gagactgagt ttgagctgac tgttttcctt gtcgaaagtg
      721 aaggcccgtc gaagcagata ttccactttg aactcagcca tggtatcgct gccctgacgg
      781 ccgctacccc gggtggagat gcgcagaacc acctctttgg ccccttgctt caattgaaaa
      841 taaaccggcc gcccctgacc ctggctgacc ttgatcaggc gaaagcccag atgatagagg
      901 ctggcatctt taacaccatc ctgactgaga ccggtatagg caatgggaat gcgccgctcg
      961 aaataacgac taccgttatc caacttgacc gaaatggtct cccgcacatg acaggttgca
     1021 ctttcctgcg cttccacttc agtatcgagc gatactatct catcggctaa agccgccccg
     1081 gcccatacaa aaaacaagac caaactgaaa cacagaccta atccggagac tcgcaaccaa
     1141 tcagtttgct tatttgacaa cttccaaagc cccataaaag acatggcaca attatataat
     1201 tccctctgcc gacacctgca caattgaaca accttggcag gacgacagag aaaaatagtg
     1261 taagagaatt cgctaaatca ataaggataa agggaccaat gacagtggac tgcctgattt
     1321 gccgcaaagc cggggatgaa gaaaatcgca ttcactatct gccccacggt ctctatcttt
     1381 atgaaagtaa gtattttcgg gtcaggcatt ccgacgaaac caaaatcgcc ggctatttaa
     1441 ttatcgaatc aaagcgccac ttcctggatc tttcggaagc caccgaagag gaagcggcgg
     1501 aattcggagt ggaactggcc cgggtggtgc gcgccgtgcg ccgcgtcaca catcccaaaa
     1561 gggtttacac cttcagcctg gctgaagcag tgccccattt tcacgtgcat gtcataccta
     1621 gagatgagaa tttaccggcc cgctatgtgg gcaggggcat tctctcctat ccgcttcagc
     1681 cttccctcaa tgaaagcacc agggacgaag tgagccaggc gctgcgcagg gttttcaaag
     1741 ccctgtcctg atctattagg caaaccccaa taaaaatata gtctagccct caacccgggt
     1801 tgccggaagt caaggcatta ttaatggtgt gtagctctgt tgtttcacac ctgtgctcaa
     1861 ctggggaaaa gacgttgatg tctgcggaat ctgaaaaaat gaatctgggt agtgagaacg
     1921 tggccatgct aggcgaactg ttctatagaa gcgggctagt gtccatcgac cagctctgcc
     1981 acgccctcta tgtcgctcat aagaatagtc tacctctagg tcgtgtgctg gtgatgctgg
     2041 gcatgaccaa aacacgcatc ttggaaggtg ctctcactct acaaaatctc gtcaggcaaa
     2101 atcttttgac accggccctg gccgttcact gcctgcaaat cctcggcacc aataaagtaa
     2161 gagtagaaga agtcctctct gaaatgggat gggaaggcaa tccccacaaa gacgaaggca
     2221 atgtgggcga aattctggtg gaagccaaaa ttctgaccag aaagagcatc gacgatgctc
     2281 tcggactgag ccgccatatt ggtctgcccc tgccccgcat cctctacctg caaggcatgg
     2341 tctcaagcaa acttctcaat gccaccatga agtgccagga tgtgatccgt tccggtcgcg
     2401 tgcagaaaga agaagcaatt caagccctgg cttcggtggc tagcatgctg gaattcgaga
     2461 aagcctgcga gaacgccctt gagaccaagc aaccttttat aaaactagaa gagttgctgc
     2521 ttctggccag gctcttatcc caaagagatc tgcaaaactt tgaagagcag atgaggattg
     2581 tgggagtcaa aaatctggga cagtacattg tcgaatgcgg ctttttctca agcactctca
     2641 tagccagcgc cgtgcgcttg cagtccatga ttgcccgttt tgagttgacg ccgctatccg
     2701 ccgccggagc gctgcgtcta attttcagcc gcaatctcag cctggctgat gcccttgagg
     2761 aaatcggcaa ggaaagaccg ccttacgacg gggaaatcag cctctatcac ctgctgaggc
     2821 tctccggctt ggtcaccgcc tttgatgtca gacgactggg gcatctaccc ggtacggccg
     2881 gtccacaact gaccgaccct atcgaagagc gcctcttcaa agccggcata ctgagcaaag
     2941 cctcaatcga cgccgccaag cgctgccagc ttctcatgag agaaggcttc ctcactgccg
     3001 aacaagcact gattgtattg cagcactggt cctggagcgg tgaaggactg accagggtgc
     3061 tggagaaatt gcactgggga ccgggcaata tgcccacctt gcatgcccag gcctttcgca
     3121 gttaactagc ctctcaccca gcccacaccc agtagctgca agcttatatt gctcttcttg
     3181 gcgcacactt cgcgaacgct aatctcggct ccactgcctg actgagccag agctgcctcc
     3241 gaggcaaatt ccgcattcaa gcgctccagt ttttcatgca gcacttgcaa gttttctttg
     3301 gcccgttcca catcttcccg ctgcttggcg gcccggttta cgcctctggc cgcagtaccg
     3361 gctctaccaa gccccatttt acgaccgaca aacgcaccaa gtacagtggc accgatggaa
     3421 agcgccgact ccatatcctg acgctttgcc tccgccgctt ccgcttctaa tttctgctga
     3481 gccagacgca ctttctcttc caattgctgt agtttcggcg cgtacttagc acgcaactgc
     3541 tgcacc
//
LOCUS       DAOQNZ010000232         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12722_length_3544_cov_3.732875, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000232 DAOQNZ010000000
VERSION     DAOQNZ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12722_length_3544_cov_3.732875"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..977
                     /locus_tag="PKW73_05445"
     CDS             285..977
                     /locus_tag="PKW73_05445"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNA72758.1"
                     /translation="MRLHNIFGKRVGLTIATTLAVLGSLLPFCTAPVLAEDAKAAAPA
                     GQTNPKKAALLKELCTLLAPGKSAESTMLAAFAEQEKQTPEIIDAIISQRAPKDATPE
                     KIAEVKKAMVETQLKVLRRTHELFSKQIDMNGLCMSIYTTVYDKYFTEPQLKQLIAFY
                     KSSAGKRLVEVQPQIATEAIRMTNKEIEGKIMSISQQVAQEMAGKAAEKSQEAPTTAP
                     SAPATAPAPEQK"
     gene            1216..1749
                     /locus_tag="PKW73_05450"
     CDS             1216..1749
                     /locus_tag="PKW73_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72759.1"
                     /translation="MKVAFTKVIGLAALGALMGVYQLAASLPAYAGGGNSRPVVSNYY
                     VAGSTAVAGWEQGLVAREPNLAKWHWSSISSSIHARNVNGGAVSVRSNPYHYSKPKVL
                     ALPVNSSIRSENQRLDGRLRQRDNGLLADARPVRSTQLSTYGDVNGRLRNSQSNFETG
                     VNGSMVDKQLTGRVLSY"
     gene            complement(1743..>3544)
                     /locus_tag="PKW73_05455"
     CDS             complement(1743..>3544)
                     /locus_tag="PKW73_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA72760.1"
                     /translation="RMRQSIYAVASFWLTAWGYRLFGLNEMAARLPFALLSSLLVFAA
                     YYTIRVVSNKKAALIAGLATASAPLLMLISKTSCIDAAFTTFLNLAVFATILALFARR
                     PVFAPVIYLSLALAVLTKGPAGLILFAMGTVVFAFLQKPKLKEIGYWIIDSRPIVGIP
                     LFLMTVLPWYYLVTKATKGLFLQVFLLYENLARFAGKTNYHKGSPLYYLPVLAYGLAP
                     WFLLLPQALKQNWFHPLWDKWFSGKLIFGFKTSYRPHARKMLNPEGSGERPQLSPEEL
                     EEKSLFYLATWALSTMAFFSLSKTQLDTYLLPAIAPFACCLAVTMTRLCQPPPQQGED
                     SLRFDRLWLLIVLGIVATFTLLAGVALVVLSLMAGKLVPTNSLSSNHFEPFLLFAAVL
                     SIILAVVQFHFLWKKAWQAQLTAAVISLATLLALLHPVGMQLYCHRNQDNMVRLALKL
                     KGSPDEVAVYGPFRPSAMSYMGRPIDTISSVDSFVKVDNLVKDGFAWGRTPSGKRQLI
                     MAADKNAADFKKRPDLTFIELTREGDWACYELLNGYAERCKTLEEAFKLYLYTKTPFT
                     VDEGRGPMTVPLGGGDRYWYKHRIKNTNTNTNQ"
BASE COUNT          823 a          882 c         1021 g          818 t
ORIGIN      
        1 cccgaatgtc tcaaatagtc gttgaagagc cgggtcttgg cctggatgta gcggtctttg
       61 cgaaagccgc gccagcctct gagccgttcc agcatctcgt ggaggctttc tgcaagcttt
      121 ccgggttgtt caatttgttg cgtcaatggc ggttttccca aagtgctctt tcatttagcc
      181 gggtcttgct ctcagtttgc caaattggtt ctatcataag ggctgtgaca gttaaatgca
      241 gactgcgtaa accagggttc gccagattat ttgggggtaa tgaagtgaga ttgcacaata
      301 tcttcggcaa aagggtcggt ttgaccattg ccacaacact tgccgttttg ggctccctgc
      361 ttcctttctg cactgctccc gtgctggcgg aagacgctaa ggcggcagcc ccggccgggc
      421 agaccaaccc caagaaggcg gctctcttga aggaattgtg caccttgctg gcgcctggca
      481 agagcgccga gtcgactatg ctggcggcct ttgccgagca ggaaaagcag accccggaaa
      541 tcatcgatgc cataatcagt cagagggctc ctaaagacgc cacacccgag aagattgccg
      601 aagtgaagaa agccatggtg gagacccagc ttaaggtttt gcgccgcacc catgaactct
      661 tttccaagca aatagacatg aacggactgt gcatgtctat ttacaccacc gtttatgaca
      721 agtacttcac cgagccccag ctcaagcaat tgattgcctt ttacaagtct tccgccggca
      781 agcgcctggt ggaggttcag ccccagattg ccaccgaagc cattcgcatg accaataagg
      841 agattgaggg caagatcatg agcatttccc agcaggtggc ccaggaaatg gccggtaagg
      901 ccgccgagaa gtctcaggaa gccccgacga cggcgccttc tgccccggca actgcgccag
      961 cgcccgaaca gaaataaatt cgccggactt tccagtcttt ttcacgctga ccgacctatt
     1021 ttatgtaggt cggtcttagc ttttagggac gatttgtaag gcaggcgctg ctttaacagg
     1081 tttgaaacaa tcgtcgattt taattagtgc attcgatgca agctatttca gcccttacta
     1141 tccaaaaact gctcaacccg tatatatagc gtcagttgcc actgccctac ttttcttcaa
     1201 tttctggagc aatctatgaa agtcgccttt actaaagtga tcggtcttgc cgccctggga
     1261 gccttgatgg gtgtttacca gttagctgcc tcgctgccgg cttatgcagg cggtggtaac
     1321 agccgtccgg tcgtctccaa ctactatgtg gccggttcca cggccgtggc cggctgggag
     1381 cagggcctgg tggcccgcga gcccaatctg gctaagtggc actggagttc gatttccagt
     1441 tccattcatg cccgcaatgt gaacggtgga gcggtcagtg tcaggtctaa tccttaccac
     1501 tattccaaac ccaaagtatt ggctctgccc gtcaacagtt ctatccgcag tgaaaatcag
     1561 agattagacg ggcgtttgcg ccagcgtgac aacggcctgc tggctgatgc cagaccggtg
     1621 agaagcacgc aactgtctac ctatggcgat gtaaatggcc gcctgagaaa cagccagtct
     1681 aattttgaga ccggtgtgaa tgggtctatg gtggataagc aattgaccgg tagagtgctc
     1741 agctattgat tggtattagt gttagtgttt ttgatgcgat gcttgtacca gtagcgatcg
     1801 ccgccgccca agggaacggt catggggcct cggccttcgt ctaccgtaaa aggagtttta
     1861 gtgtagagat agagtttgaa ggcttcttcc agggtcttgc agcgttcggc atagccgttc
     1921 aaaagttcgt agcaggccca atcgccttca cgcgtcaatt ctatgaaagt caagtcgggg
     1981 cgcttcttga agtcggcggc gtttttgtcg gcggccatta tcaattgtct tttccctgat
     2041 ggggtgcgtc cccaggcgaa gccgtctttc accaggttgt ctactttgac gaaggagtcc
     2101 acactggata tggtatcaat gggacgaccc atatagctca tagctgatgg tcggaaaggt
     2161 ccatagactg ccacctcgtc cgggctgcct ttgagtttca gggccagccg caccatattg
     2221 tcctgattcc tgtggcagta gagttgcatg cccaccggat gcaggagggc tagtagtgta
     2281 gccaggctta tcactgcagc ggtgagctgg gcttgccagg cttttttcca aaggaagtga
     2341 aattgcacca ctgccaggat aatgctcaaa actgccgcaa agagcaggaa gggctcgaag
     2401 tggttgctgg agaggctgtt cgtaggcacc agcttgccgg ccatgaggct gaggacgacc
     2461 agcgccactc ccgccaggag agtgaaagtg gcaactatgc cgagcacaat gagaagccag
     2521 agtctgtcga agcgaaggct gtcttctcct tgttgcggcg gcggctggca gagcctggtc
     2581 atggtcaccg ccaggcagca agcgaagggg gcgatagccg gcagcaagta agtatccaac
     2641 tgggttttag agaggctgaa gaaagccatg gtggaaagag cccaggtggc caggtagaaa
     2701 agacttttct cttccagctc ttccgggctt agctgcggtc tttcgccgct tccttccggg
     2761 ttgagcattt tgcgggcgtg ggggcggtaa ctggttttga agccgaatat gagtttgccc
     2821 gaaaaccact tatcccagag ggggtggaac cagttctgtt tgagggcctg gggcagaagc
     2881 aggaaccatg gtgccagtcc gtaagccagt acaggcagat agtagagggg tgagcctttg
     2941 tggtaattgg tttttccggc aaagcgagcc aggttttcat agagaaggaa gacttgcaga
     3001 aagagtccct tggttgcttt tgttaccagg tagtaccagg gtaagaccgt catcaggaat
     3061 agcgggatac cgactatagg acggctgtct atgatccagt aaccgatttc cttcagtttt
     3121 ggtttttgca agaaggcgaa aactactgtg cccatggcaa acaaaatcag accggccgga
     3181 cctttggtaa gtacagccag ggccaggctc aggtaaatga ccggggcaaa aacgggacgg
     3241 cgagcaaaca gtgccaggat ggtagcgaag acggccaggt tgaggaaggt agtaaaagcg
     3301 gcatcaatac aggaggtttt gctgatcagc atcaagagcg gtgccgatgc cgtggccagt
     3361 ccggcgatca aagcggcttt cttattggat acaactctga tggtgtagta agcggcgaag
     3421 acaagcaggc tggacaggag agcaaaaggc aggcgggctg ccatttcatt cagcccgaag
     3481 aggcgataac cccaggccgt cagccagaaa gaagccacgg cgtaaatgga ttgacgcata
     3541 cgcc
//
LOCUS       DAOQNZ010000233         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12783_length_3534_cov_3.772636, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000233 DAOQNZ010000000
VERSION     DAOQNZ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12783_length_3534_cov_3.772636"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKW73_05460"
     CDS             <1..668
                     /locus_tag="PKW73_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72761.1"
                     /translation="KDVGPLLQDLPEKATAFKETIAAFLSGIETIDKKGDTVTILRNS
                     QVKHNLGGVDLVLNPCLTLKFSPSNRAPAIDKLGGVTVMLPVPGELGLGDNLPVPIDS
                     LRLSPKYGGQRDLTLGTGGIVADYVKVKVDGNLQAVTDASGNYLVKTRLMNPLGGQKD
                     KLNLNLRFDQQGRLNMLPSELLDIVSAASAQGADLSLSGAGKVLLSGGTKLAATAGWL
                     FGY"
     gene            complement(774..1898)
                     /gene="hppD"
                     /locus_tag="PKW73_05465"
     CDS             complement(774..1898)
                     /gene="hppD"
                     /locus_tag="PKW73_05465"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNA72762.1"
                     /translation="MVKEATKLKEANPVEEINIDGFDYVEFYVGNALQACYYYNRGFG
                     FDLVGYRGLETGSRDCVSYVMEQDHVKIVLTGAMQAKHEVAEHVKQHGDGVKSIGMRV
                     RDVKACYDTAIARGAKSVYEPKTVEDEHGTYISAAVATYGDTIHTFIDRSNYKGFAPG
                     FKTILGKPGNSVGLVHIDHIVGNVEADNLQKWVDFYSQIFGFHIFQYFDAEDISTKYS
                     ALVSKVMANKSGTIKMPINEPYEAGLRKSQIQEYIDYYNAPGVQHIAITTRDIIATVG
                     ELRKRGIEFLSVPQTYYDTLTERVGKIDEDINALAKLGILVDRESEGYLLQIFTKPVQ
                     DRPTLFFEIIQRKGATGFGKGNFMALFESIEREQAQRGNL"
     gene            complement(2156..2806)
                     /locus_tag="PKW73_05470"
     CDS             complement(2156..2806)
                     /locus_tag="PKW73_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72763.1"
                     /translation="MALLLTGSLSACSGRVDTGGMNLSRVEKGEGLQVAGGKEVSTSQ
                     VDANGSFKFDMAAEEQVATQSVAAWRKALAGDEKGAVAQLEALEKRFPQVLTIQMMLG
                     QVKDHFGKKEEALEHFRRAARGNQFSTMASYKLAEALRETGRHDEAIPHYRRLIKGAT
                     NYDVSLREPFDFGLAYCLLRKDPHSQEGLKLLDDCLKARPDHKDALALKAELAGKH"
     gene            complement(2859..>3534)
                     /locus_tag="PKW73_05475"
     CDS             complement(2859..>3534)
                     /locus_tag="PKW73_05475"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72764.1"
                     /translation="LLLACLASTHSVAFAQGAGSGHKAVSHHQGLSEHVEDIGALKMK
                     VEAEPGDAKLRYSLAEALRKAGQNKEAAREYLQVTESEPTFYHAYHQISVISSDKHIL
                     DEALGRLNFLKDERPKDLLLRVALSEIYEKKGDYFNASKVLIDLVYLNEVPEKYAQKV
                     NNRIRLMQAKAKDSHSLDKTYVGDEQMENAPPPLPEATLGRDLSISKVKEPRVMQGFG
                     HATLLP"
BASE COUNT          842 a          967 c          891 g          834 t
ORIGIN      
        1 caaaggacgt ggggcccctg ctgcaagacc tgccggaaaa agctacggct ttcaaagaaa
       61 ccatcgccgc tttcctttca ggcatcgaaa caattgataa gaaaggggac acggtgacaa
      121 tactgagaaa cagtcaggtt aagcacaacc tgggtggagt agacctggtg ctcaatccct
      181 gcctgaccct taagttcagc ccctccaacc gggctccggc catagacaaa ctgggaggcg
      241 tcacggttat gctgccggtt cccggcgagc ttggtctggg tgataacttg cctgtgccaa
      301 ttgactccct gcgcctcagc cccaagtacg gcgggcaaag agatctgact ctgggcacag
      361 gcggtatagt tgccgactat gtgaaagtga aagtagacgg caatctgcag gcagtaaccg
      421 atgcaagcgg caactacctg gtgaaaacgc gcctcatgaa cccgctgggt ggtcaaaagg
      481 acaagctcaa tctcaatctg cgcttcgacc aacaaggaag gctcaatatg ttgccctcgg
      541 agcttctcga cattgtctcg gcggcatcgg cgcaaggagc ggatttgagc ctgagcggcg
      601 ccggcaaagt gctcctttcc ggcggcacca aactagcagc cacagccggc tggcttttcg
      661 gctactagca aaagttatgg tcgccctcat ggcactgccg gtcaatgaca gaccaacaaa
      721 aaagcaggtc gccctgcttt tttgccttta gactacgctt gccaaagaca accttagaga
      781 ttgcctcttt gagcttgctc cctttcgatt gattcaaaca gggccatgaa gttgccttta
      841 ccgaaaccgg tggcaccttt gcgctggatg atttcgaaga agagagtcgg tctatcctgc
      901 accggcttgg tgaagatctg cagcaagtag ccttccgact cccggtcaac cagaataccg
      961 agcttggcca gggcattgat gtcttcatca attttgccca cccgttcagt caaggtatcg
     1021 taataggttt gcggcactga cagaaattct atgccgcgtt tgcgcaactc accgaccgtg
     1081 gcgatgatat cgcgggtggt gatagcaata tgctgcacgc ccggggcatt gtaatagtcg
     1141 atgtattctt gaatttgcga cttgcgcaga ccggcttcgt aaggctcgtt gatgggcatc
     1201 ttaatggtgc cgctcttatt agccataact ttggaaacca gagccgaata cttggtgctg
     1261 atatcttcgg catcaaagta ctggaagata tggaagccga agatctggct gtaaaaatca
     1321 acccatttct gcaaattgtc ggcttcgacg ttgcctacga tatggtcgat atgcaccaga
     1381 ccaacgctgt tgccgggctt gccaagaatg gtcttgaagc cgggagcaaa gcccttgtaa
     1441 ttgctgcgat ctataaaggt atgaatagtg tcgccatagg ttgctacggc agcagagatg
     1501 taggtaccgt gttcgtcctc aaccgtctta ggctcataga cgctcttggc accacgggcg
     1561 atagcagtat cgtaacaagc tttcacatcg cgcacgcgca tgccgatgct cttcactccg
     1621 tcgccgtgct gcttcacatg ctcggccact tcgtgcttgg cctgcatggc accggtcaat
     1681 acgattttga catggtcttg ttccataacg taagaaacgc aatcgcggct gccggtctcc
     1741 agcccgcgat acccgaccag atcaaagcca aagccgcgat tgtagtagta acatgcttgc
     1801 agagcgttgc ccacatagaa ctctacatag tcgaaaccat caatattgat ctcttccacg
     1861 ggattagctt ctttgagttt ggtagcttct tttaccatcg gaatctcctc gcattgactc
     1921 ctttccagaa gagaatttct cctgaatata ggatagcgat attgtacttc agcattgctt
     1981 aagaattagc ctggcaagca gatcttagtc aaaactaagg agctgagcca ggctttaagt
     2041 acaggcagaa gccgccctcg acgcccttca acagcaagcg tccaaaaact aacgcagtag
     2101 aaagcgcctt accgcctgaa taccgggcac aaaaaggtaa ccggtttttc ctcactcagt
     2161 gcttgccggc tagctccgct ttcaaagcca aggcatcttt atgatcggga cgagctttga
     2221 gacagtcgtc cagaagcttc aaaccttcct gcgaatgcgg gtctttacgc aacaggcagt
     2281 aggcaagacc aaaatcaaag ggctcacgca aggacacatc atagttggtc gcccccttga
     2341 tcaaacggcg ataatgggga atcgcttcgt cgtggcgacc ggtttcacgc aacgcttccg
     2401 ccagcttata agaagccatg gtgctgaatt gattgcctct cgccgcccgg cgaaagtgtt
     2461 ccagggcctc ttccttctta ccaaaatgat ctttcacctg accgagcatc atttgaatgg
     2521 tcagcacttg tggaaagcgc ttttcaagcg cttctaattg tgcaacggca cccttctcgt
     2581 ctccagccag agccttgcgc caggccgcca ccgactgagt agccacctgt tcttccgccg
     2641 ccatatcaaa cttgaaactg ccgttagcgt ccacctggct ggtagacact tccttgcctc
     2701 cggccacttg cagcccctcg cccttttcca cccgagacag attcataccg ccggtatcaa
     2761 cccgtcccga acaggcgctc aaactgcccg tcagaagcag cgccacagac cccgcaagaa
     2821 gaaaacgaac agacttgatt gaatttgcca gagcggcact agggcaataa tgtggcatga
     2881 ccgaatcctt gcataactct tggttccttc accttggaaa tggacaggtc acgaccgagg
     2941 gtcgcttccg gcaagggcgg cggcgcattt tccatctgct cgtcaccgac ataggtcttg
     3001 tcgagggagt gagaatcctt ggctttagcc tgcatcaagc ggatgcgatt gttgactttc
     3061 tgggcatact tttcgggcac ttcattgaga taaacaagat cgatcaagac cttggaagca
     3121 ttgaagtaat cccctttctt ctcataaatt tcagagagcg ccacgcgcaa gagcaagtct
     3181 ttcggtcttt cgtctttgag gaaattgagg cgcccgaggg cttcatcgag gatgtgtttg
     3241 tcgctggaga tgaccgaaat ctggtgatag gcgtgataga aagtcggctc gctctcggtg
     3301 acttgcaaat attcccgggc tgcttctttg ttttgtccgg ctttgcgcaa agcttccgcc
     3361 aggctgtagc gcaattttgc gtcaccgggc tcggcttcta ccttcatctt caaggcaccg
     3421 atgtcttcaa catgctcact gagaccctga tgatgactga ccgctttatg gccgctgccg
     3481 gcgccctggg caaaagcgac actatgggtg gaagccagac aggctagaag cagt
//
LOCUS       DAOQNZ010000234         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12794_length_3533_cov_3.215929, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000234 DAOQNZ010000000
VERSION     DAOQNZ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12794_length_3533_cov_3.215929"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            535..2235
                     /locus_tag="PKW73_05480"
     CDS             535..2235
                     /locus_tag="PKW73_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNA72765.1"
                     /translation="MSLDKQLPVENVDALKGVDASAVKVDVQAQLSKPADISAANQFF
                     KGNLSPSADKFLPSLDTALPVGADAGLGAAPGLTPGAEQMSPLIQMIMKMPGHISLVG
                     SFFEVLGAFFAPMQEALTNVFDPSMFNIDAGGMLDGDNMAFLDADGGDFSAALDALPA
                     DAPILQGLPGGEGPFGQLASAIAPRDLGHSLFHGSSLSDSFMNAPSLEVSGPMSPGKS
                     LFEGANNLNFAPTSGEFGAYTAMGPQVDPSGNFGSTVGNSNGYNYQQNMPMQQQGQPS
                     PYQNYYQNGAGQMPQDGMNQAGSGQEQMPQGEAQQPGEQQAQEASESTTPYTVKSGDN
                     LWDIAREHLGDGTRWPEIYKLNEGIIGDNPRLIMPGAELQLPGGASDLAQADYTVKPG
                     DNLWDISKEHLGGGEHWPELYKENTGTIGSNPDLIHPGDKLHMPAGDSLAHNQSPVHT
                     TAAAPHHQASSAVGNHHPGAAGHHANHVAHKPEAMHQAGHGQEAAGTALKHATPHPVA
                     SATPPTAVPHAASAPAAAAELPPRQASLPADSSPIIAPQDNLENLSLSAKAQSLESGT
                     "
     gene            complement(2239..2643)
                     /locus_tag="PKW73_05485"
     CDS             complement(2239..2643)
                     /locus_tag="PKW73_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNA72766.1"
                     /translation="MVTAIAEADKQEVQEAIESTRAGRVFVPRADIFESQDKLVVLAD
                     MPGVDESSVEITVENNILTLQGFVKAPVRDGYRPVLTEYGVGDFRRSFTLSNAIDREG
                     IEAACKNGVLTLTLPKSKSLLPKKVTVKAVSD"
     gene            complement(2719..3186)
                     /locus_tag="PKW73_05490"
     CDS             complement(2719..3186)
                     /locus_tag="PKW73_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNA72767.1"
                     /translation="MLLQRISDGRFADNSLSNFWRSLSELDAARHSLNRLLTGDSAAG
                     TSSEFPALNVWLSEDDALVEAELAGIEAEDIDISVTNDSLTLRGSRPAYALAEGERFH
                     RQERGSGQFSRSLQLPFRIDADNVEANLSKGVLQIRVPRAESDKLKKIMVRPA"
BASE COUNT          844 a         1039 c          920 g          730 t
ORIGIN      
        1 ggaactattt cgcgaagtct tggcctgctt ctccgggaca gcgtactggg catctgctgc
       61 taacgcttgc gcttccaaca tagcaagcga cgctgggacc atcgtaggta tgtaaggcgg
      121 aggcgggggg acaagataat ttttggctgg gggggaactg tttcgcaagg ttttcgcctc
      181 cacagactgc gtgcccaggg tgaacgaccc gctgaaagcg acggctagcg aaatgagtgc
      241 tacaagtttc ctgatcaaat gaaagtcccc cggaagatgg ccgctttggt tatgcagtgg
      301 cgcgcgagcc caggccacac agttcataaa ccattatgca gagcgcttgt gatggtggcg
      361 tgatcagata agatgcgagt ctcgacattt ttgcgggaga gccaagattt agccagaaag
      421 gcttaactaa caatgatttc gggacccgtt caagatttca cttgacatcg ccccggcacc
      481 ggctcaataa tggggggctt cctgttttca gctcccgctt cgaacttact attaatgtca
      541 ctagacaagc agctacccgt ggaaaacgtg gacgctctca agggcgttga tgcttctgct
      601 gtaaaagtag acgtgcaagc acagttatcg aagccggccg atatctcagc cgctaatcaa
      661 ttctttaagg gcaacctctc acccagcgcc gacaagtttc tgccctccct ggataccgcc
      721 ctgccggtgg gagcggacgc cggtctcggt gccgccccgg gtctgacacc gggagctgag
      781 cagatgtccc cgcttattca gatgatcatg aagatgcccg gtcacatcag tctggtgggc
      841 agcttcttcg aggtcctggg cgcctttttt gcccccatgc aggaagctct caccaatgtc
      901 ttcgacccct cgatgttcaa cattgatgcc ggcggcatgc tggacggcga caatatggct
      961 tttctggacg ctgatggcgg tgactttagc gccgcccttg atgccctgcc ggcagacgcg
     1021 cccatcttac aaggtctacc aggtggcgaa gggcccttcg ggcaactggc ctcggctatc
     1081 gcccccagag acctggggca ttcgctcttc cacggctcaa gcctctccga cagcttcatg
     1141 aatgctccca gcctggaagt gtcaggaccg atgagcccag gcaaatcact gtttgaaggg
     1201 gcaaacaacc tcaacttcgc tcccacgtcg ggagaattcg gagcatacac agccatgggc
     1261 ccgcaggtgg atcccagcgg caacttcggc tctacggtag gaaactcaaa cggctacaac
     1321 taccagcaaa atatgcccat gcaacagcag ggtcagccct ccccatacca gaactactac
     1381 cagaacggcg ccggtcagat gccgcaagac ggcatgaacc aggccggttc cggccaggaa
     1441 cagatgcctc agggcgaagc ccaacagcca ggcgagcaac aggcgcaaga agcaagcgag
     1501 agcaccacac cctacacggt caaatccggc gacaatctct gggatatagc ccgcgaacat
     1561 ctaggtgacg gcacgcgctg gcctgaaatc tacaaattga acgaaggcat tatcggtgac
     1621 aatcctcgct tgatcatgcc gggcgcagaa ctgcaattac caggcggcgc aagcgacctg
     1681 gcccaggcag actacaccgt caagccgggc gacaacctct gggacatctc caaagagcat
     1741 ttaggcggcg gcgaacactg gccagaactc tacaaagaaa ataccggcac catcggctcc
     1801 aaccccgatc tgattcatcc cggcgacaag ttgcacatgc cggcaggcga ttccctggcc
     1861 cacaaccaga gccctgtgca cactaccgcc gcggcaccgc accatcaagc cagcagcgcc
     1921 gtcggcaacc atcatcccgg tgccgccgga caccatgcca accatgtagc ccataagccg
     1981 gaagcaatgc atcaagctgg tcatggtcag gaagcggccg gaaccgctct caaacatgcc
     2041 acaccgcatc cagtggcatc ggcaactccg ccgacagccg tgccccatgc tgcatcagca
     2101 ccggcagcag cggcagaact gccgccgcga caagcatccc tgcctgcgga cagcagcccc
     2161 atcatcgcgc cgcaagacaa tcttgagaat ctcagtctca gcgccaaggc tcaatcgctg
     2221 gaaagcggca cctgacgctt agtcagaaac agccttgacg gtcacctttt tgggcagaag
     2281 tgacttgctc ttcggtaggg taagagtcag gacgccattt ttacatgcgg cttctatgcc
     2341 ctcgcgatca atggcattgg aaagggtgaa agagcgcctg aaatctccca ctccgtattc
     2401 cgtcagtacc gggcggtaac cgtctctgac aggagctttg acaaagccct gcagggtgag
     2461 aatattgttt tccacggtaa tctcgacaga gctttcatcg accccgggca tgtcagccag
     2521 caccaccagc ttgtcttgag attcaaagat atcggcgcgc ggcacgaaaa ctcgcccggc
     2581 gcgggtgctt tcaatcgctt cctgcacttc ttgtttatcg gcttcggcaa tagcagtaac
     2641 catgatgttt actccttctg tgcttctttc gaacaaccaa tcaattcact tttttgagac
     2701 tacgggcggt gcgcttcctc aagccggccg taccataatt ttcttaagtt tgtccgactc
     2761 ggcccggggc acgcgaatct gcaaaacccc cttgctcaag ttggcttcta cattgtcggc
     2821 atcaattcta aaaggcagtt gcagactgcg actgaactga ccgctgcccc gctcctgacg
     2881 atggaacctt tctccttcag cgagcgcata ggcaggtctt gagccccgca aggtcaaact
     2941 gtcattggta acggagatgt cgatatcttc agcttcgata ccggcaagtt cagcttccac
     3001 cagggcatca tcttcggaaa gccagacatt gagagccggg aactccgaag aggtgccggc
     3061 agccgagtca ccggtcagca agcggtttaa actgtgcctg gcggcatcca gctcactgag
     3121 tgacctccag aagtttgaga gactgttgtc ggcaaatctt ccatcagaaa ttctttgcag
     3181 caacatagca gtattcctcc atcagcttta atccaaggga aatattgttt ttgatatgtc
     3241 agcgccgccc tttaaggctc taagctcttg caagggatgg agcgaacaat tatttttggc
     3301 attggtgcta tcaaatcggg gcaccgtttt gaatataccg aaggggccga aactgctccg
     3361 gcaaaaatag tcttccctta ataattacct taagggccgt ttgcaagggc ttggcaaaag
     3421 ctctaattgc cgacacaata gagatcttca attctatggg ccaggttaag caatgccgga
     3481 gagctgggaa ctacacaaac tgttcgccca taacgcagag tccaacggtg act
//
LOCUS       DAOQNZ010000235         3524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12851_length_3524_cov_3.404728, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000235 DAOQNZ010000000
VERSION     DAOQNZ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12851_length_3524_cov_3.404728"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PKW73_05495"
     CDS             <1..1281
                     /locus_tag="PKW73_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA72768.1"
                     /translation="HQDRAYEEQSLHYALMNTRAGSKAKRLFLKLACQNLLRKPTRTM
                     LLIAAVAMVTGSVFASTIVGRGVQASMERSFAKMGADLIVVPAQALVNITSALLTVQP
                     TEATLDTTLAEKIKTIPGVEKVATQTIYQIPMMVHMPEHKANLIAFAPESDFTILPWL
                     KDKPDRPLRKGDLISGSRREETLGEELEPCGRAAKIYGRLGISGVGPLDDNLFASYET
                     AQYLAEGTVEGKQAIANFDPKRCSAILVKLSTGTTPEQVRFAVSQLNDVKVVMGTNIV
                     TSTRQTTSALLLGLLGLALMLLLGASIVLSLLFSAIISERKREIGLLSAIGSRRSNII
                     GMFLAEACLTTGLGAIGGIGLGGALIMVFQRSLIYYLETLQIEFYWFSAAEIAQIALL
                     CMGLTILCGLLGALVPAWKVSREEAYSLIQSEGG"
     gene            1285..2712
                     /locus_tag="PKW73_05500"
     CDS             1285..2712
                     /locus_tag="PKW73_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA72769.1"
                     /translation="MEVQVSQLSKSYALENTGRSGAGGKTATLTKTDAVQEVNFTIAS
                     GTFLAIVGRSGSGKSTLLAMLGGICRPSSGNIKLGDKSLWEESESKRADLRNKEIGYV
                     FQFASVMPSLRAIDNVALPALVSGLLSEKAAYARARALLIQVGLNERIDSYPGELSGG
                     EQRRVAIARALINAPKLLLADEPTADLDEETEAEILNLLIEIKQAHNLTMILVTHNND
                     IAAHADRVLSMKKGRATMSDQQSAAELNASRERLENIFAISSKQVETEKVSLGLGIDR
                     YVGRFVLVLIPVLSLIYIINLALAGYQQSLATARQEEQQAMEDLAMSDLKAGVKAIKM
                     PGNDSYELEVFLRNSRGDKPMFVLKPAVRVFVQLSNNWLETTAEAIDPPGTDVMQIKG
                     EQLFRYKIKPTVAGYTQLIPYYMHVRITNDMLVSGSKTPKKDLIERKDSYYVYLKPYG
                     VADEAIAAKLKFAGAPPVYLPMPPH"
     gene            complement(2743..>3524)
                     /locus_tag="PKW73_05505"
     CDS             complement(2743..>3524)
                     /locus_tag="PKW73_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72770.1"
                     /translation="GYLKFVPATESTSQTPVTQLSYLVDSALTLRRESSVVMGDPNKK
                     KAIPLLERQLAILEQANGKHGPKLQGILAQLAACYVSVGDDASAMKTYKRYFEIVDKD
                     GDYWSDQVVTVAYCDLLTRNHRAKEVPAFLRSRQLTPETSSKVPSSPLYILMIEAMAR
                     QNMKETAAEHLRVWFKAPEYPVWDAEIVAQQHSQQPYTFEGHYYVLPPVSAPPAQKPE
                     VEDAFGNYPVMTEVSGSKKLLQACAEPTGLKSKAPTSFSGF"
BASE COUNT          904 a          940 c          922 g          758 t
ORIGIN      
        1 caccaagacc gagcatatga agaacagtcg ctgcactacg ctctaatgaa cactcgcgcc
       61 ggaagcaaag caaagagact ctttctcaaa ttggcctgcc agaatttgct gcgcaaacca
      121 acccgcacca tgctcttgat cgcagcagta gccatggtga cagggtccgt ctttgcatcc
      181 accatcgtcg gacgcggcgt gcaggcttcc atggagagaa gctttgccaa aatgggagcg
      241 gatctaattg tggtgcctgc ccaggcgctg gtaaatatca ccagcgcact actgacggtg
      301 cagcccacgg aggccactct cgacaccact ctggcggaga aaatcaagac cataccgggt
      361 gtggaaaagg tagccacgca aactatctat cagataccaa tgatggtgca catgccggag
      421 cacaaggcaa acctgatagc ctttgctccg gagagcgact ttaccatttt gccatggctc
      481 aaggataagc cggacagacc actgcgcaaa ggtgacctga taagcggttc caggcgcgag
      541 gagacactcg gggaagaact ggagccctgc ggcagggccg caaaaatata cggcagactt
      601 ggcataagcg gcgtgggccc cctcgacgac aatttgttcg ccagctatga aaccgctcaa
      661 tatctggcag agggcacggt agaaggcaag caggccattg ccaacttcga cccgaaaaga
      721 tgttcggcca tactggtaaa gctatcaacc ggcaccacgc ccgagcaggt cagatttgcc
      781 gtctcgcaac tcaatgatgt gaaagtggtt atgggtacca acatagtgac ttccaccaga
      841 caaactactt ccgccctgct gctcgggcta ctgggactgg ctctcatgct cttgcttggt
      901 gcatcaattg tgctgagctt gcttttctcc gccattattt ccgaacgcaa gagagaaata
      961 gggctacttt ccgccatcgg ttcaaggcgc tcgaacataa tcggcatgtt tttagcggag
     1021 gcttgtctga ccacagggct gggagccatt ggtggtatcg gtcttggtgg cgcgctgata
     1081 atggtctttc aaagatctct aatttactat ctggaaacct tgcaaataga attttactgg
     1141 tttagtgccg cagaaattgc ccaaatagct ttactttgca tgggtctgac catactttgc
     1201 gggctactag gggcccttgt gccggcctgg aaagtgagcc gcgaagaagc ttacagcctc
     1261 atccagagcg agggaggcta gaccatggaa gtacaggtct cacaactctc caaatcttac
     1321 gccctcgaaa acaccggcag aagcggtgcc ggcggtaaaa ccgccacact gaccaagacc
     1381 gatgcagtgc aagaggtgaa tttcacaatc gccagtggaa ccttcctggc tatcgtcggc
     1441 cgctccggct ccggcaaatc taccttgctg gccatgctcg gcggcatctg ccggccaagt
     1501 agcggcaata tcaaactggg cgacaagagt ctctgggaag agagcgaaag taagcgcgcc
     1561 gacctgcgca ataaagaaat cggctacgtc tttcagtttg ccagcgtcat gccttctctc
     1621 agggccatag ataacgtggc actgccggcg ctggtttccg gtctgctcag cgagaaggcg
     1681 gcctatgcca gagccagggc cctgcttatt caagtaggtc tgaatgagcg catcgactcc
     1741 tacccgggtg agctatcagg tggagagcaa agacgtgtgg ccatagcccg ggcgctcatc
     1801 aacgcaccaa aactactgct tgccgatgaa cctaccgccg acctcgatga agagacggaa
     1861 gcggagattc tcaatctgct cattgagata aaacaggctc acaatttgac catgattctg
     1921 gtcacccaca acaatgacat tgccgcccat gccgaccgcg tgctgagcat gaagaaaggc
     1981 cgggcaacaa tgagcgacca gcagtctgcc gccgaactga acgcaagcag agagaggctg
     2041 gagaatattt ttgccatctc ttccaagcag gtagaaaccg aaaaagtaag cctggggctt
     2101 ggcatcgatc gctatgtggg gcgctttgta ctggtattga taccggtgct ctctcttatc
     2161 tacattatca atctggccct ggccggctac cagcaaagcc tcgccactgc caggcaggaa
     2221 gaacagcagg ccatggaaga cctggccatg agtgatttga aggcaggagt gaaggcaatt
     2281 aaaatgccgg ggaacgactc ttatgagttg gaagttttct tgcgcaattc caggggcgat
     2341 aagcccatgt ttgtactcaa gcccgctgtc agagtctttg tgcaactatc aaacaactgg
     2401 ctggaaacga cagccgaagc catagaccct cccgggactg atgtaatgca gatcaagggc
     2461 gaacaactat ttagatacaa aataaaaccc acggtggccg gctatactca gttgattccc
     2521 tattacatgc acgtgcgcat caccaacgac atgctcgtga gcggcagtaa aacaccgaaa
     2581 aaagatctaa tcgaacgcaa agacagttac tatgtctatc ttaagcccta tggcgtagcc
     2641 gacgaagcca tcgccgccaa gctaaagttt gccggtgcac cgcctgtata cttaccgatg
     2701 ccgccccatt gaaagaagga agcatcagca atgtaaggcg gattaaaagc cggaaaatga
     2761 agttggtgct ttcgacttaa gaccggttgg ctcggcacaa gcttgcagaa gtttcttgct
     2821 gcctgaaact tccgtcatca ccggatagtt cccaaaggca tcttcgacct ccggcttctg
     2881 tgcaggaggg gcggaaaccg gcggcaagac ataataatga ccttcaaaag tgtacggctg
     2941 ctgagagtgt tgctgagcaa ctatctcggc atcccaaaca gggtactccg gggccttgaa
     3001 ccagacccgc agatgttccg ccgctgtctc cttcatgttt tgtctggcca tggcttctat
     3061 cattaaaata tacagcggcg aactgggaac tttactactc gtttccggag tgagctgccg
     3121 tgatcttaga aaggctggca cttccttagc acggtggttt ctggtcaata agtcacaata
     3181 ggccacagtc accacctggt cagaccagta atctccatct ttatcgacaa tttcaaagta
     3241 tcgcttgtaa gttttcatgg cgctggcatc atcaccgacc gaaacataac aagctgccag
     3301 ttgcgccagg attccttgca atttaggtcc gtgcttaccg ttggcttgct ccagaatggc
     3361 cagttgccgc tcgagcagag gaatagcttt cttcttgttc ggatcgccca tcaccacaga
     3421 cgactcccgc ctgagagtaa gggctgaatc caccaaataa gagagttgcg ttacgggagt
     3481 ttgcgaggtg gattccgtgg cgggtacgaa ctttaagtag ccgg
//
LOCUS       DAOQNZ010000236         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12876_length_3520_cov_2.996825, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000236 DAOQNZ010000000
VERSION     DAOQNZ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12876_length_3520_cov_2.996825"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="PKW73_05510"
     CDS             complement(<1..1598)
                     /locus_tag="PKW73_05510"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNA72771.1"
                     /translation="MAEKQVRGRGHGFDEHIVVRGARQHNLKNIDLKIPRGNLVVVTG
                     VSGSGKSSLAFDTIFAEGQRRYVESLSAYARQFLGQLDKPDVDGIDGLSPAISIDQKS
                     TSHNPRSTVGTVTEIYDYLRLLFARVGTPHCPKCDRLINPQTIDQIVDQVLSLDEGSK
                     IQILAPLIAGKKGEYQSLFQELRKEGFVRCRIDGEVVELEDEEQIKLDKNKKHNIDLV
                     VDRLVIKKGIQSRLADSLSLALKWGKQNALVDIVGGSTTKKGDLLFSENFACAACGVS
                     FAELTPRIFSFNSPYGACDECHGLGCTYEVDPALVVPDGKKTLEEGAIYPWAKTNNPY
                     YDQLIASVAKHYKFSVKVPFEKLTKAQQGIILFGSGAERIKLGHDSFDGREFWEYKKS
                     FEGVINNLKRRHEESQSDRIKQDIENYMTQMPCASCNGSRLKQESLAVKVQSMSIAQV
                     SALSVEKAVTYFQSVPESLSEKQRTIAHQVLLEINARLKFLTDVGLTYLTLDRQAGTL
                     SGGEAQRIRLATQIGSGLSGVLYVL"
     gene            1859..>3520
                     /locus_tag="PKW73_05515"
     CDS             1859..>3520
                     /locus_tag="PKW73_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72772.1"
                     /translation="MWPGSKGKGQKGKDGSPKDGDLKKARATKAKLKAMTKEALEAEQ
                     EAAFRERAQEKSAQSFNDSEDLDEKDEVDGEVNESEVGLNYHQETREAEGGNFIRLPA
                     SAGEHSNEKVSGVWKNRVLGTGRPAAQDSQSQTGPRSVVDQNERIRQNAQHEAMVNLV
                     DRMFDYFQNLAYDFNQMNPAPELELSWIRPTLTRENISSWHQSAQYISVFTGRISTRF
                     WTLVVRGTYEAVDSYIIPADKLLSFTTAPTSHNCALSILPAFDGSRVFYYAHDHLLDE
                     DTLHLCYRALLDTLITVAQDDQPLRGSLDLDALGVFGSVSHAGPTASASAPVGREAEM
                     EIDYSALYRQHMAAQGQSQPQASPQKTFSQADAGAPVSGEFYGGQGQSTPPQTYPPEF
                     STSAPPTAHAAPPVGQNVSGSQNSLQSQAPAAGSGRDDDDWKPVVRSVRSASQQMPIN
                     PFNTATPPEAPKPASSGAGEWRDVSSSGSRPAVDPNASGFNLQARPDALSSSGSRPAV
                     DPNASGFNLQARPDAPRPGFSAQALFQSASAGMPQTPVQPASPPAA"
BASE COUNT          747 a          960 c          974 g          839 t
ORIGIN      
        1 agcacataga gcacgccgga aaggccggag cctatctggg ttgccagccg aatgcgctgg
       61 gcttcgccgc cggaaagagt accggcttga cgatccaggg tgagataagt gagtcccaca
      121 tcggtgagga atttcaaacg agcgttgatc tcaagcaaga cctgatgggc tatggtgcgc
      181 tgcttttcgc tcaaactttc cggcacggac tgaaaatagg tgacggcttt ttctacggaa
      241 agagctgaga cctgggcaat ggacatactc tgcaccttga cggccaggct ttcctgcttg
      301 aggcggctgc cgttgcaact ggcgcacggc atctgcgtca tgtaattttc gatgtcttgc
      361 tttatgcggt ctgattgcga ttcttcatgg cgtcgcttga ggttgttgat tacaccttcg
      421 aaggattttt tgtattccca gaattctcgg ccgtcgaaag agtcatgacc gagtttgatg
      481 cgttctgcgc cggagccgaa gagaatgatg ccctgctggg cttttgtcaa tttttcgaaa
      541 ggtactttga ctgagaattt gtagtgtttg gctacggagg cgatgagttg atcgtaatag
      601 ggattattgg tcttggccca ggggtagatg gctccctctt ccagggtctt cttaccgtcc
      661 ggcactacca gcgccgggtc tacctcgtat gtgcagccca gtccgtggca ttcatcacag
      721 gcgccgtaag ggctgttgaa ggaaaagatt cgcggggtca attcggcaaa gctgactcca
      781 caggcggcac aagcaaagtt ctcggaaaag agaagatctc ctttcttggt ggtgctaccg
      841 ccgacgatat caaccagggc attctgtttt ccccatttta gggcgaggga aaggctatcg
      901 gccagcctgg actggatgcc tttcttaatc accagtctgt ccaccaccag gtcgatgttg
      961 tgtttcttgt tcttgtcgag ctttatttgc tcttcatctt ccagttccac aacttcgccg
     1021 tcgatgcggc atctgacaaa gccttccttg cgcaattctt gaaagagcga ctggtattct
     1081 cccttcttgc cggcaatcaa tggggcaagt atttgaatct tgctgccttc gtcgagagaa
     1141 agcacctggt cgacaatctg gtcgatggtc tggggattga tcaggcggtc gcacttggga
     1201 caatgggggg ttcctacccg ggcgaataag agcctcaggt aatcatatat ttccgtaacg
     1261 gtgccgactg tggaacgggg attatggctg gtggatttct ggtcaatgga gatagccggt
     1321 gagagcccgt cgatgccgtc cacgtccggt ttgtccagct gtcccaggaa ttgtctggcg
     1381 taggctgaga gtgactccac atagcgtctc tgtccctcgg cgaaaatagt gtcgaaagcc
     1441 agggacgatt taccggagcc gctcacacct gtgacaacca ctaaattgcc ccggggaatt
     1501 tttaagtcga tgttcttaag attgtgctgt ctggctcccc gcactacgat gtgctcgtca
     1561 aagccatgac ctctgcctcg aacttgcttc tccgccacgc tgacacccct gaaaatgatg
     1621 ttacggttaa atattatccg ccattgcagc tgttttcctc accaagttgc ggcggctaga
     1681 cgggcttgtg aataaagaga agcattaaaa ataacgtgct tttggggtgg ggcattatta
     1741 attagggtta tatgggcgct cggcaagggt agatcctggt taggcgggct tggcggcgct
     1801 gacggaggct accactggcg tcatccgtcc agcacttttc tcgggaggca accaggatat
     1861 gtggccaggt tctaagggta agggccaaaa aggaaaagat ggctcaccca aggacggtga
     1921 tctcaaaaag gcaagggcta ccaaagctaa gctgaaggcc atgaccaagg aggcgctgga
     1981 ggctgagcag gaggctgcct ttcgcgagcg tgcccaggaa aagagcgctc aatctttcaa
     2041 cgattcggaa gatctggatg agaaagacga ggtggatggt gaagttaatg agtccgaagt
     2101 cggcttgaat tatcaccagg agaccagaga agccgagggc ggcaatttca tacgactgcc
     2161 tgcttcggcc ggcgagcaca gtaacgagaa ggtctcaggc gtctggaaaa atagagtgct
     2221 gggcaccggc agacctgcgg cccaggattc tcaatctcaa accggtccgc gatcggtggt
     2281 tgaccagaac gagcgcatca gacagaacgc tcagcacgaa gccatggtta atctggtgga
     2341 tcgcatgttc gattacttcc agaatctggc ttacgatttc aaccagatga atccggctcc
     2401 cgaattggaa ttgagctgga tccgaccgac tctgacccga gagaacatca gcagttggca
     2461 ccagagtgcc cagtacattt ccgttttcac cggccgtatc tccactcgct tctggaccct
     2521 ggtggtgcgc ggtacctatg aggcggtaga ttcgtacata attcctgccg acaaattgct
     2581 ctctttcacc acggctccca ccagtcataa ttgcgctctt tctatcttgc cggcatttga
     2641 cggttcgcgc gttttttatt acgcccacga tcacctgctt gatgaagaca ccctgcacct
     2701 ttgctaccgc gccttgcttg acaccttgat cactgtcgct caggacgacc agcccctgag
     2761 aggctctcta gatctcgatg ctctcggggt gtttggctcc gtctctcacg ccggtcccac
     2821 cgcctcagcc agtgctcctg tgggccggga ggcggaaatg gaaatcgatt attctgctct
     2881 ttacaggcag cacatggcgg ctcagggaca gagtcagcca caagcgagcc cgcaaaagac
     2941 cttcagccag gctgacgctg gggcaccagt gagcggggag ttctatggcg ggcagggaca
     3001 gtctactcct ccccagacat atcctcccga gttttccacc tcggctcctc caactgctca
     3061 tgcggcacca cccgttgggc agaatgtctc cggctcccaa aattctttgc agtcgcaggc
     3121 accggccgcc ggctccggtc gcgacgatga tgattggaaa ccggttgtac gctcggtgcg
     3181 cagtgcttcc cagcaaatgc ccatcaatcc ttttaatacc gccactcctc ctgaagcgcc
     3241 caagcctgcc agttccggcg ccggtgaatg gcgagatgta tcttcatcag gttccagacc
     3301 ggctgttgac cccaatgcct ccggcttcaa tctgcaggcg cgtccggacg ctttatcttc
     3361 atcaggttcc agaccggctg tggatcccaa tgcctccggc ttcaatcttc aggctcgtcc
     3421 ggacgctccc cgtcctggtt tttccgcgca ggctctcttc cagagcgcca gcgccggcat
     3481 gcctcaaact ccggtacagc ctgcgagccc accggcggcc
//
LOCUS       DAOQNZ010000237         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12882_length_3519_cov_2.937644, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000237 DAOQNZ010000000
VERSION     DAOQNZ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12882_length_3519_cov_2.937644"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PKW73_05520"
     CDS             complement(<1..1007)
                     /locus_tag="PKW73_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72773.1"
                     /translation="MSMAACIHSLEGNAKNFIEELEQAMSNLYKTAASSRQYDGDLAV
                     ATLALSFLLTATQIGFVADGLMMVSGQGWFLSLLFAIVIATGLLHSQFLMNRLFEKSL
                     PRRYAWLFGGLTAAITGLMSVWGMTRLSEAWTYKWAMGIFLGTLVPLQTTLLANMTTG
                     MFALAGRNWYKGSLCEKFFAVVSTSRAEVAQAAEARPAQAPEETLGASSVDTAAASLA
                     PPPIAAPEPVPSPAPLQASPREERLAEEPQPTLSSAPPSQDQSRYHERSHNSPILNSE
                     SRIASFSFSEERVSIRIKSHLSKRQWKDLHSRLRRVASITWDDVEKTTSQDGQRERIL
                     TG"
     gene            complement(1542..1931)
                     /locus_tag="PKW73_05525"
     CDS             complement(1542..1931)
                     /locus_tag="PKW73_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72774.1"
                     /translation="MAESRQAEFDRAKEKFEQQCPSSIAVEAWSADCKAISNKNARDT
                     ADNFTAVFPDGASLMKEFGKNPNDVITPKEMAAKIDELTNSKNDASAFQKADVVSLLS
                     IAVGQTQNPNLTAERYDKYIANLRKGI"
     gene            complement(2053..2439)
                     /locus_tag="PKW73_05530"
     CDS             complement(2053..2439)
                     /locus_tag="PKW73_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72775.1"
                     /translation="MPSRVANTSSVRAMALPMPSSCDLIEAAVIGLSLSMIAIVSGNK
                     LTDWLGSAAVFITFLHGQLSFAMQESQGKMAQPTVENYHWSGRLFVTKEILWIATFAL
                     AGCWPLCAGSVLFATYPHWRALLRRS"
     gene            complement(2633..>3519)
                     /locus_tag="PKW73_05535"
     CDS             complement(2633..>3519)
                     /locus_tag="PKW73_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA72776.1"
                     /translation="SGNYPYTNYLGTTPMTSVILLLALAAITWLALSPRLCPKFYHRF
                     LFHPHKELGEASEIETLVQTYGGTEYFFRTKAGHTLHAFSLGELAEKRPVLFYSMGKN
                     GDIPRRAEILAQLLKLPLTVFIYEYRGFGLSQGQPNLPGLVEDARESYDFLCQTLKVE
                     AERIVLFGESLGGAVTAQLAGSRKAAAIITKSTFASLNQIARHQFPLLRLYPERLMLC
                     PDWHSAQALARVNIPTLIVHGNKDRMIPVDHAHQIHQICSHCSRLLLLPEARHSFMTD
                     ADNESFAAGLTAFLNDLK"
BASE COUNT          771 a          823 c         1050 g          875 t
ORIGIN      
        1 ccggtgagaa tgcgctcgcg ttgtccatct tgggaggtag tcttttcgac gtcgtcccag
       61 gtgatagagg caactcggcg taaccgggag tgtaagtcct tccactggcg cttggacaga
      121 tgactcttga tgcgaatcga cacacgctcc tcgctgaagg agaaggaggc gattcggctt
      181 tcggagttaa ggatgggact gttatgcgac cgttcatggt accggctctg gtcttggctt
      241 ggcggcgcac tgctgagggt aggttgcggt tcttcagcca gcctctcctc cctggggctt
      301 gcctgaagcg gagccggtga aggcaccggt tccggagcgg caatcggggg tggggccagg
      361 gaagcggcag cggtatctac cgaagacgct cccagtgtct cttccggcgc ctgtgcaggc
      421 ctggcttcgg cagcttgcgc tacctctgcc cgggaggttg acaccacggc aaagaacttt
      481 tcgcagaggc tacctttgta ccagttgcgg cccgctagcg cgaacatacc ggtagtcatg
      541 ttggccagaa gggtagtttg cagaggcacc agggtgccca gaaagatacc catagcccat
      601 ttatatgtcc aggcttcgct caagcgggtc ataccccaga ccgacatcag accggtaatg
      661 gcggctgtca gaccaccaaa gagccaggca taacgtcggg gcagagattt ttcgaagagc
      721 ctgttcataa ggaactggct atgcaaaaga cccgtggcga tgacgatggc gaagagaagg
      781 gagagaaacc aaccttgtcc gcttaccatc atcagaccgt ctgctacgaa accaatttgt
      841 gtagcagtaa gtagaaaact tagcgcgagg gtggccacgg ccaggtcacc atcgtactgc
      901 ctgcttgagg cagcagtctt atagaggttg gacatggctt gctccaattc ttctataaag
      961 tttttcgcgt tgccttccag agagtgaatg caagcggcca tactcacgga tacttcctgg
     1021 tccttcttta gtgaagggga gtcgtgagta tggcggccgg cacgcagtta aactggtaag
     1081 gtgaatactt taataacggc tatccatggc tagacactta tggtcttgcg aaattgctga
     1141 agctagcgat ggtggctgga atagtttggt tggttgggtt ctgctcttgg gggtgacgga
     1201 aaacgatatc tgcaatctgc cccatcggcg gtgccggagt caaggtgatt ggcgtttcat
     1261 ttttggcggt cgggcaagaa tttattttgc gaacgtacct tagtatggaa ccgtcgagca
     1321 agccggctgc cggagccctt cgtgggctgg cctagcttcg cttggttggc gcagctggcg
     1381 ctagaaatgg taccgggggt ggtggcaaaa acggcaaagc gagctacgtc gacatggtcg
     1441 acggctcgca actgcttttc gattgacagg ctctattagt gcgcaatttg cgtgaaaaag
     1501 atgtgaaaga caaaggtgcg aatgagccta ttcgaacttt cttaaatgcc cttgcgtaaa
     1561 ttggctatgt atttgtcgta gcgttctgca gttagattgg gattttgtgt ttgaccaact
     1621 gcaatggaga gcaagctgac tacatctgcc ttttgaaatg cgctggcgtc atttttagag
     1681 tttgtcagct catctatctt ggctgccatt tctttggggg tgatgacatc gttcgggttc
     1741 ttgccgaatt ctttcatgag gctggcgccg tccgggaaga ctgccgtaaa gttgtcggct
     1801 gtgtctctag catttttgtt ggagatggct ttgcagtccg cactccaggc ttccactgct
     1861 atgctggagg ggcactgttg ttcgaatttc tctttcgctc tatcaaattc tgcttgtctg
     1921 ctttctgcca tgattgatac tccggggtag gttgagttca ccttaccggt tgtctgtggc
     1981 gaagagtggg cgggctagtg tcggcgccgt gactgcggct gcggacttaa tgccaggagt
     2041 ttcttgtgct agtcaggaac ggcgcaagag tgcccgccag tggggatagg tggcgaagag
     2101 cacactgccg gcgcagagag gccagcatcc tgccagggcg aaagtggcaa tccagagaat
     2161 ttctttggtg acgaagagtc tgcccgacca gtgatagttt tcgacggtgg gctgtgccat
     2221 tttgccctgt gattcctgca tggcaaaaga aagctgaccg tgcaggaagg tgatgaagac
     2281 agcggcgctg ccaagccagt cggttagctt gttgccggag acaatggcaa tcatggaaag
     2341 ggagagtcct atcaccgctg cttcaatcag gtcgcaggag gagggcatag gtagcgccat
     2401 ggctctaact gaacttgtgt tggcgacgcg gctcggcatg gcaaaatcct cccagtgtgg
     2461 gctgggtagc agtctggtaa aaaagaacta gttagaacat gccacagggt gactgtggtt
     2521 aaacacttga tcccggcaaa tagtagaccg gtcagttaat tagtcgcaaa aagagataga
     2581 gagcagatct cgccggcttg ggcagatctg ctctactgat tggagggtgt tgttatttga
     2641 ggtcgttcag aaatgcagtc agaccggcgg caaagctctc attgtctgca tccgtcataa
     2701 agctgtgtcg agcttccggg agcagtaaga gtcttgagca gtggctgcag atctggtgaa
     2761 tttggtgggc atggtcaact ggaatcatgc ggtctttgtt gccgtgcaca atcaaagtcg
     2821 ggatattgac ccgggccagt gcttgagcgc tgtgccaatc cgggcagagc atcagtctct
     2881 ccgggtagag acgcagcagc ggaaactgat gtctggctat ctggttcaag ctggcaaagg
     2941 tagactttgt aattatggcg gcggcctttc tgcttcctgc cagttgggct gtcaccgcac
     3001 cccctagtga ttcgccgaat aagacaatgc gctcagcttc gacttttagg gtttggcaaa
     3061 ggaagtcgta actttcccga gcgtcttcca caagaccagg caaatttggc tgaccctgac
     3121 tcagtccgaa accgcggtat tcgtaaatga agaccgtcaa tggcagcttc aacaactgtg
     3181 ccagtatttc cgctcttctt ggtatgtcgc cgtttttgcc catgctgtag aagagtactg
     3241 gtctcttttc tgccaattct cccagactga aagcgtgcag agtatgtccg gctttggttc
     3301 gaaagaagta ctcggtgccg ccgtaagttt gaaccaacgt ctcaatctcc gaggcttcgc
     3361 ccaattcctt atgtggatga aagaggaagc ggtgataaaa cttcgggcag agccttggtg
     3421 acagggcaag ccatgtaatt gccgcaaggg caagcaataa aataactgat gtcattggtg
     3481 tggtcccaag atagtttgta tatgggtagt taccggacc
//
LOCUS       DAOQNZ010000238         3512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12935_length_3512_cov_4.018802, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000238 DAOQNZ010000000
VERSION     DAOQNZ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12935_length_3512_cov_4.018802"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKW73_05540"
     CDS             <1..817
                     /locus_tag="PKW73_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNA72777.1"
                     /translation="RQVGVPHIVVFLNKCDMVDDPELLDLVEMEARELLDKYNFPGDK
                     IPFIRGSALKCLEGDASAEKAVLELMKAVDEYIPTPERDIDKPFLLAVEDVFSITGRG
                     TVATGRIERGQIKVGEEVEIVGLQETRKTTVTGVEMYQKTLDSGIAGDNVGILLRGID
                     KTMIERGQVLAKPGSIKPHTKFKAEVYVLSKEEGGRHTPFFPGYRPQFYIRTTDVTGS
                     ITLPAGVEMVMPGDNVAMDVELIQPVAVEEGMRFAIREGGRTVGAGVVASIIA"
     gene            complement(826..1089)
                     /locus_tag="PKW73_05545"
     CDS             complement(826..1089)
                     /locus_tag="PKW73_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72778.1"
                     /translation="MSTSKKTGENESHKERGCFSFNFLLGGVGMGNHDEACISNHPST
                     EAAKLKITAIFKVAKRTADNRHMSHFQAQKKGWSIGPTLFIAF"
     gene            1094..1756
                     /locus_tag="PKW73_05550"
     CDS             1094..1756
                     /locus_tag="PKW73_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72779.1"
                     /translation="MLATFSTDVLMHPNLHPRLFGFFHYLTHYHPRLCRLLVYGLFSA
                     YLGLVFLAPLVPRLSSWRMRKNGLLSDEKLRLTTYLNCPRLEKYPLVEPELWQRLGKV
                     LPGLVIKLPDLRAAHWQMKAVDFSRKEFFLELWYVPNPLVIMVSRLYPRKLKCRARVI
                     SKGTSSAVEFCFSADSAMDYENVRGIIGSTMVQLRERLEATEELSSVESLGVSLFPVF
                     TR"
     gene            complement(1949..>3512)
                     /locus_tag="PKW73_05555"
     CDS             complement(1949..>3512)
                     /locus_tag="PKW73_05555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72780.1"
                     /translation="IDRCMNRGEILGRALKKTAKEKPRTIAYRPLAGLEKWLRARFAN
                     NPRRSNWKLILLLVVTLTLILLDPGKQMSDLIVALTATGAAPQGYGTAAGAVAAAGSI
                     DWKHLFVSTFACTAVGTLLAYLTMLLRFPRALLSCSQGLGYRFSRLSGKKTKFLPWTQ
                     ITHIGIEKKKGSTSHKDDNLIFKTGKKTLSLKLSGFETADDREALLAEIEKYAPRVPR
                     DPEVAEYLSKPPDRSYTELWLQALSAPPKRERLKPLFKDALLQNGRFQVREELGSGGQ
                     GFAYLATDRDGRHVVLKEFVLPIYVDIQARKKALERFENEARLLSKLDHPQVVKLQHF
                     FVEDHRAYLVLEHIDGKNLRQIVNEKGAFSQAEVQELALKMCEILKYLHGLTPPLVHR
                     DFTPDNLILDREGNLKLIDFNVAQEMQEATTGTVVGKQSYLPPEQFRGQACPASDLYA
                     LGASIFFILTGKEPTPISTIKLQEEAKEGQSFQEIFCALLEQLTDMEPSRRPSSEAVS
                     ELLAASEVEGSR"
BASE COUNT          753 a          929 c          923 g          907 t
ORIGIN      
        1 ccgtcaggta ggcgtacccc acatcgttgt attcctgaac aagtgcgaca tggttgacga
       61 tccggaactt ctggacctcg tcgaaatgga agctcgcgaa cttctcgaca aatacaactt
      121 ccccggcgac aagattcctt tcatccgcgg ttccgctctc aagtgtctcg aaggagacgc
      181 ttctgctgag aaagccgttc tcgaactgat gaaagccgta gacgaataca tccccacccc
      241 cgagcgcgat atcgacaaac cgttcttgct cgctgttgaa gacgtattct ccatcaccgg
      301 tcgtggtacc gttgccaccg gccgtatcga gcgcggacaa atcaaagttg gcgaagaagt
      361 agaaatcgtt ggtctgcaag agacccgcaa aaccaccgtt accggtgttg agatgtacca
      421 gaagacattg gattccggta tcgccggcga caacgtcggt atcctcctgc gcggtatcga
      481 taagaccatg atcgagcgcg gtcaagtgct cgctaagcct ggttccatca aaccccacac
      541 caaattcaag gctgaggtat acgtcctctc caaagaagaa ggtggtcgtc acactccgtt
      601 cttccctggt tacagaccgc agttctacat ccgtacaact gacgtgaccg gctccatcac
      661 tttgcctgcc ggcgttgaga tggttatgcc tggtgacaac gttgctatgg acgtcgagct
      721 catccagccc gtagccgttg aagaaggtat gagattcgct atccgcgaag gcggtcgtac
      781 cgtaggcgcc ggtgttgtag ccagcatcat cgcctagtag gcagcttaaa aagcaatgaa
      841 aagggttggt ccaatggacc agcccttttt ttgtgcctga aaatggctca tgtggcgatt
      901 gtcagcggtc ctcttggcaa ccttaaaaat agcagtaatt ttcagctttg ccgcttccgt
      961 cgagggatga ttcgaaatac atgcttcatc gtgattgccc atgccgaccc caccaagcag
     1021 aaaattgaaa ctaaagcagc ctcgctcttt gtggctctcg ttttcacctg ttttcttgct
     1081 ggtgctcatt attttgctgg ccactttcag caccgatgtg ctcatgcatc ccaatctgca
     1141 tccgcgcctc tttggttttt ttcactattt aactcactat cacccgcgcc tgtgccggct
     1201 actggtttac ggtctctttt cggcctacct gggcctggtc ttcctggctc cgctggtgcc
     1261 ccggctttca tcctggcgca tgagaaaaaa tggtttgctt agcgacgaga agcttagatt
     1321 gacgacttat ttaaattgcc ctcgcctgga gaagtatccg ctagttgagc ccgaactctg
     1381 gcagaggctt ggtaaagtac tgccgggact tgtgataaag cttcccgatc tgcgggctgc
     1441 ccactggcag atgaaggcgg tcgatttcag ccgcaaagag tttttcctgg aactctggta
     1501 cgtaccaaac ccactggtga taatggtctc ccgcctctat ccgcgcaagc tgaagtgccg
     1561 ggccagggtc atctcaaaag gaacctccag tgccgttgaa ttttgctttt ctgcagatag
     1621 cgctatggat tacgaaaatg tccgtggcat cattggcagt accatggttc agttgcgaga
     1681 gcgattagaa gcgacggagg agttgagcag tgttgagtct cttggagtta gtcttttccc
     1741 tgttttcaca agatagggct gaaagaaagg acagtaatta ctttcttgtg atggtttctg
     1801 tgtttattgt cattggcctc gtcgcggtcg ctgcgacagt atatgccaat cgcgccggtc
     1861 tgtctttgct tccctccgca gccctgggca aactcgccca atgagggtga gctggttggc
     1921 gcaactccac agttcatctt gcccgatttc accgacttcc ctctacttcg gaggcagcaa
     1981 gcagttcgct tacggcttca ctgctgggtc ttctgctcgg ctccatgtcg gtcaactgtt
     2041 ccagtagggc gcagaatatt tcctgaaagc tttgcccctc ttttgcctcc tcctggagct
     2101 ttatggtgga tattggtgtg ggctctttac cggtcagtat gaagaaaatg ctggctccca
     2161 gggcataaag atccgaggcc gggcaagcct gtccgcgaaa ctgttcgggc ggcaggtaag
     2221 actgcttgcc cacaacggta ccggtggtag cctcctgcat ttcctgggct acgttgaaat
     2281 ctattagttt gagattgcct tctctgtcta agatgagatt gtccggcgtg aaatcgcggt
     2341 gcaccagggg cggtgtaaga ccatgcaggt atttgagaat ttcgcacatc ttcaaggcca
     2401 gttcttgcac ttccgcctgg ctgaaggcac ccttttcatt cacgatctgt cgtagattct
     2461 tgccgtcgat gtgttcgagc accaggtagg cccggtggtc ttccacgaag aagtgctgta
     2521 gtttcaccac ctgtggatgg tccaacttcg ataaaagccg ggcttcgttt tcaaagcgct
     2581 ccagggcctt ctttctggct tgaatatcta cataaatagg caaaacaaat tcctttagaa
     2641 ctacatggcg cccatctcta tcagttgcca ggtaggcaaa gccctgaccg cctgaaccaa
     2701 gttcctctct tacttgaaag cggccgtttt gcaataaggc atctttgaaa agcggcttca
     2761 atcgctctcg cttgggcggc gccgagagcg cttgcagcca gagttccgta taacttcggt
     2821 ccgggggctt agacaagtat tccgctactt ccggatctct tggtacacgg ggggcgtact
     2881 tctcgatttc ggcaagcaag gcttctctgt catcggccgt ttcaaagcca ctaagcttta
     2941 aagagagggt ctttttgcca gtcttaaaaa tcaggttgtc gtccttgtgg gaagtgctgc
     3001 ccttcttctt ctctatgcct atatgggtga tctgagtcca cggtaggaat ttggttttct
     3061 taccgctcaa tctggagaag cgatagccga gaccctggct gcaacttaga agcgcccggg
     3121 gaaagcgcag taacatggtc aggtaagcaa gtaaggtacc gacagcggtg caggcaaaag
     3181 ttgagacaaa gaggtgcttc cagtctatgg agccggctgc agcgaccgca ccggcagcgg
     3241 tgccatagcc ctgaggggcg gcaccggttg ccgtcaaagc tacgatcagg tcgctcatct
     3301 gcttgcctgg atcgaggaga atgagagtga gggtgacaac cagcaaaaga atgagcttcc
     3361 agttgctgcg cctggggtta ttggcgaagc gcgctctcag ccatttctca agaccggcca
     3421 gggggcggta ggcgatggtc cgcggtttct ctttagctgt ttttttgagg gcgcgaccca
     3481 gtatctcacc gcgattcatg caccggtcta tt
//
LOCUS       DAOQNZ010000239         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12951_length_3510_cov_3.018813, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000239 DAOQNZ010000000
VERSION     DAOQNZ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12951_length_3510_cov_3.018813"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKW73_05560"
     CDS             complement(<1..583)
                     /locus_tag="PKW73_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNA72781.1"
                     /translation="MTEQIEHKIQVFNTLTGKKEDFVPLSGKTVKIYACGPTVYDSSH
                     LGHARKEIVWDTVQRYLRFLGYDVIYVRNITDVDDKIINRAKERGIGPDRLAREYTYA
                     FWRDMDALNVASPDFEPRATEFISQMIAFVQDLIKKGHAYESGGDVYFDVASFKDYGK
                     LGKQNLEQLLVGARDQVIAQETLSQLKKSPVDFA"
     gene            complement(624..2135)
                     /gene="gltX"
                     /locus_tag="PKW73_05565"
     CDS             complement(624..2135)
                     /gene="gltX"
                     /locus_tag="PKW73_05565"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNA72782.1"
                     /translation="MSTANPQSTAHLEEASKGAGKVRVRIAPSPTGHLHVGTARTALY
                     NYLYAKRHNGTFVLRLEDTDEVRSRDEFTHDIIDGLKWLGLSWDEGPDIGGPYPPYKQ
                     TEKVDHYEHFADKLIRDGRAYLCYATAEELALLKEDQKETGQAPRYDNRGRDLTDAAR
                     TKYEAEGRIPSVRFIVDEPRMVSWQDSIKGEISIDTSDLGGDMVIIKSTGTAIYNFAV
                     VIDDIDMKMTHVIRGEDHIHNTAKQLLIYEALAINPPVFAHAPLIFDLERQKLSKRKH
                     GETVHIDYYRKMGYMPEAIVNYLAQMSWTHPEGKEIYTLDEVAPLFDLDKVSKSPAVF
                     DVARLNWFNGHYIRSLALSLITERAKPFLSQAGFNLSAYEGEQIEQIVGSVREGLTTL
                     WELPDAVRFFFAEKPELSEEVKTTVLAGEKPAQVLKTLKDKLADMPWGDAPALKKAVD
                     AMGKELALKGKDLYWPIRAALSGKTQGPDLGVTLAILGRERVLVRLESALSHC"
     gene            complement(2300..3142)
                     /locus_tag="PKW73_05570"
     CDS             complement(2300..3142)
                     /locus_tag="PKW73_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNA72783.1"
                     /translation="MKNKFATTTLMALCLSMTLSVSQAKALPANLEPEVESSVWEEND
                     QECASVTLNGKELIAFRAVGNSESLEDQAENLADKLQELLKDDKVDASRLVPAQEGNL
                     AAIHLDGNTVLTFTPPAGQDIQPIEHSFKLVNAMRNVLGIQAIPQSFLKMAESQGADQ
                     AYVALSRSTVAAPVNFSADFSGQASWYGGKFHGRRTSNGDVFDQDGFTAAHRTLPFGT
                     KLLVMNRRTGDSCVVEVNDRGPFCGERVIDLSRGAANKLNMLSSGVATVDCMVLPVEK
                     IASK"
BASE COUNT          703 a          897 c          992 g          918 t
ORIGIN      
        1 gggcgaagtc caccggactt ttcttcagtt gagagagcgt ctcctgggca attacctgat
       61 ctcgggcgcc gaccagaagc tgctccaggt tttgcttgcc cagtttgccg tagtctttga
      121 aagaagctac gtcaaaataa acgtcgcctc ctgattcgta agcatgacct ttcttgatca
      181 ggtcttgcac gaaggcgatc atctggctta tgaactcggt ggctctcggc tcgaagtcag
      241 gggaagccac attgagggcg tccatatccc gccagaaggc ataggtgtat tctctggcca
      301 gacgatccgg cccgatgccc cgctctttgg cgcggttgat aatcttgtcg tctacgtctg
      361 tgatgttgcg cacgtagatg acgtcgtaac cgagaaagcg cagatagcgc tgcactgtgt
      421 cccagactat ctctttacgg gcatggccta agtgactgga gtcgtatacc gtaggtccgc
      481 aagcgtagat ttttacggtc ttgcctgaaa gcggcacgaa gtcttccttc ttgccggtga
      541 gggtgttgaa tacctgaatt ttgtgttcta tttgttcagt cactggaata tctctgggtt
      601 gtcggtagct ctcaattgcc ccttcaacag tggctcagcg ccgactccag tctgacgagc
      661 acgcgctccc gtcccagtat ggcgagagtt acacccaggt cagggccctg ggtcttgccc
      721 gagagggctg cacgaatcgg ccagtaaaga tctttgccct tcaaggcaag ctctttaccc
      781 atggcgtcta cggctttctt cagcgccgga gcgtcgcccc agggcatgtc cgccagcttg
      841 tctttcaagg ttttgagaac ctgggcaggt ttttcaccgg ctagcactgt ggtttttact
      901 tcttcgctaa gctcgggttt ttcagcaaag aaaaagcgca cggcatcggg cagttcccag
      961 agagtggtga gaccctctct cactgaaccg acgatttgtt cgatttgctc gccttcgtat
     1021 gccgagagat tgaagccggc ctggctgaga aagggtttgg ctctctctgt aatcaggctc
     1081 agggctagac tgcgaatata gtgtccgttg aaccagttca agcgtgccac gtcgaagacg
     1141 gccgggctct ttgagacctt gtcgagatcg aagaggggag ctacttcatc cagtgtgtag
     1201 atttccttgc cttccggatg tgtccagctc atctgggcca ggtagttgac tatggcctcg
     1261 ggcatataac ccattttgcg atagtagtcg atgtgcaccg tttccccatg cttacgcttg
     1321 gagagttttt gcctttccag atcgaagatg aggggggcat gagcgaagac cggggggttg
     1381 atggccagtg cttcgtatat gagcagttgt ttggccgtat tgtgaatgtg atcttcaccg
     1441 cgaatcacat gggtcatttt catgtcgatg tcgtcgatga ccacggcaaa gttgtaaatt
     1501 gccgtgccgg tagatttgat aatgaccatg tcgccgccca ggtctgaggt atcgatggag
     1561 atttcaccct tgatactgtc ctgccatgac accatgcgtg gttcgtcgac gatgaagcgc
     1621 accgaaggaa tgcgaccttc cgcttcgtat ttggtgcggg ctgcatcggt gagatcgcga
     1681 ccgcgattgt cgtagcgtgg cgcctgccct gtttctttct ggtcttcttt caagagagcc
     1741 agttcttccg ctgtggcgta gcagaggtag gcacgcccgt ctctgattag tttgtcggca
     1801 aaatgctcgt agtggtctac cttttctgtc tgcttgtaag gcgggtaggg tccgccgata
     1861 tccggacctt catcccagga cagaccaagc cacttgaggc cgtcgattat gtcatgggta
     1921 aattcgtccc gtgagcgcac ctcatcggtg tcctcaagac gcaacacaaa agtgccgttg
     1981 tgacgtttgg catagagata gttgtaaaga gcagtgcggg cagttcccac atgcaaatgg
     2041 ccggtaggac tgggcgcgat acgcacgcgc accttaccgg cgcctttgct ggcttcctca
     2101 aggtgggctg tgctttgtgg attggctgta ctcatgcttg acctgtcttg cccggtgctt
     2161 gccctgaagc cgccgggggc tttggggaga ccccaggcag acattctaca gcaagcaagc
     2221 accttgttga tggcgccgag gcttccacct tcaccattag ttgcgtgtca tcacgtttgt
     2281 taacgaaaga gagggctctt tacttagagg cgattttctc taccggcaag accatgcagt
     2341 ctacggtagc cacgccggaa gaaagcatat tgagcttgtt ggcagcgcct ctggaaaggt
     2401 cgatgactcg ctctccgcag aaaggaccgc ggtcgttaac ttccacgacg caggagtcgc
     2461 cggtgcgtct gttcatgacc aaaagtttgg tgccgaaggg cagggttctg tgtgctgctg
     2521 taaagccgtc ttgatcgaag acatcaccat tggaggtgcg tctgccgtgg aacttgccac
     2581 cgtaccagga agcctgaccg ctgaagtcgg ctgagaagtt aaccggtgcc gcaacggttg
     2641 agcgggaaag ggctacgtaa gcctgatcgg cgccctggct ttcggccatc ttcaggaaag
     2701 attgagggat ggcctggata ccaagcacat tgcgcatggc attgaccaat ttgaaactgt
     2761 gctcgatggg ctgaatgtct tgtccggcag gaggggtgaa agtcaaaacg gtattgccgt
     2821 ccagatgaat ggctgccaga ttgccttcct gggcaggtac caggcggctg gcgtccacct
     2881 tgtcgtcttt cagtaattct tgcagcttgt cggccaggtt ctcggcctgg tcttccaggg
     2941 attcactgtt accgacggct ctgaaagcaa tcagttcttt accgttcaat gtaacggaag
     3001 cgcactcctg gtcgttttct tcccatacgg aagattccac ttccggttcc aggttggccg
     3061 gtagagcttt tgcctgactg acggataggg tcatagacag acacaaagcc atcaatgttg
     3121 ttgttgcaaa cttgtttttc attcggtgtc ctaactcttc ttaagtagct gtcgccctca
     3181 gtgccgggca gcaagcgttc taattgagtt tttctccttc tagtgaggta tcgcaccggt
     3241 ttcagatatt tggtggatag cctcttggcg gcttgtccga agcaggcggt aaggtaacag
     3301 aagcgccgct ctccagtcaa agatctcatg gcaagctgtc ttcccccgcc ctgttgcata
     3361 cacgcaggct ttatgggaga aacttaaggg tcttggcctt gacagtaaga taactgtcga
     3421 taagcccttg gcaagcgagc agatcttgtg ctcggtgttt tgttcccgct gatgagtcgg
     3481 atttaatttt gtgacaccac cggcgatgtc
//
LOCUS       DAOQNZ010000240         3509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12956_length_3509_cov_3.404169, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000240 DAOQNZ010000000
VERSION     DAOQNZ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12956_length_3509_cov_3.404169"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PKW73_05575"
     CDS             <1..943
                     /locus_tag="PKW73_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNA72784.1"
                     /translation="GSGKPGANPLSLPGRFWTYLQERFPLPSFVLMSMVFSLAGLSLS
                     SSLRLGSCRLSLFHCLAAMLTTVFLFFQLRVIDEHKDRLNDARYLPERPVPRGLISLK
                     ELSNLALALMSLQLAFALLAGKVVVLCLLASWVYMWLMGREFFLSSYLKERPILYMLS
                     HMLIMLFVDLLITSYDFGPSNGFATGLLWFFAASFMSGMVVEFGRKIFAPECEREGIV
                     SYSGYLGLKQAALWLALFIIAAQVFLTVCLSHLGQPLAAGLAFVIYDLLCFSALFLGF
                     KKGGPTVSKVFAALSNMSVLFGYLVVLLNCTMHSGVN"
     gene            946..3372
                     /locus_tag="PKW73_05580"
     CDS             946..3372
                     /locus_tag="PKW73_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNA72785.1"
                     /translation="MIAEIYFADGANCDICGGKARGLSRLKHISGSAGARFQVPPFFV
                     IAAGEVAVAKLQGALTTIKAESGCRSFAVRSSCIEEDGPTNSFAGQFTSYLFVEESQV
                     GLHVEKVRHSVKSKSIETYRKLKGLDGSGPVAVLVQAMVDASVSGVMFSLDPVTQKAC
                     PVISMVEGAGDKLMAGTVSGETLYIAGSTPAPGSARPSYLTEAMLAQLLRALGAIEKH
                     FGRPMDVEWCFDREGRLFILQARPITTVDPQSTVFDASNIQESYPGKTSPLTFSFARK
                     CYTEVYKSFVLLMGVKPSVVESRSDLFEYMIGYHEGRIYYNLGNWHRLIQLLPLYKSN
                     KGFMEQMMGLKEPLPQEPQAAPSNFFGKLGGLFCLAKILFAALTLPLLKRSFEKRLNL
                     ALNIDRQAVKNLSLTELASLYLSLESRLISRWDAPVVNDFFAMIAYGALRKSLSGNPK
                     RMECLPFYLKCKSAVVSGQPPLLLKELAGLISADRELIRLMESGKTGAARLRLKQHPE
                     AERLYCLYLEKFADRSLGELKLESACLEDDPSLLFSTLAALARAEKKAAVETLPPPAL
                     EPLPWPLALLASLTASLLAGRENLRFARTRVFGLVRLIFKTMGKRLAASGHIEQPQDI
                     YFLTVDEILGFIGGTGVSLNLAGLVSLRKREYEGASESPLRLTFSGAYGDRKELPRAV
                     PEEAVKASPHTSVLVGQAASAGICRGRVRVIRNPESEALQEGEILVAERTDPGWIVHF
                     SLAAGLVVSHGSLLSHTAIVARELSLPCVVAVENATTRLKTGDLVEVDGRNGKVTILE
                     EAVSNLFACA"
BASE COUNT          707 a          927 c          989 g          886 t
ORIGIN      
        1 aggcagtggc aagcccgggg ctaaccccct gtccttgccc gggcgcttct ggacttatct
       61 acaagaacgc tttcccctgc cctccttcgt gctcatgtcc atggtattta gcctggcggg
      121 gctcagtctc agtagttcat tgcgcctggg ttcatgccga ttgagcctct ttcattgcct
      181 ggccgccatg ctcacaactg tctttctttt cttccaactg cgggttatcg acgagcacaa
      241 agaccgcctc aacgacgccc gctatcttcc ggaaagaccg gtgcccaggg gtctcatttc
      301 cctgaaggaa ctctccaatc tggcccttgc tcttatgagc ttgcaattgg cttttgccct
      361 tctggccggc aaggtggtgg tgctttgcct tttggctagt tgggtctata tgtggctcat
      421 gggcagggaa ttctttctgt cgagctattt gaaagagcgg ccgatcttgt acatgctgag
      481 ccatatgttg attatgctct ttgtcgactt gctcatcacc agttacgatt ttggtccgag
      541 caatggcttt gccacaggct tactctggtt ctttgccgcg agcttcatga gcggcatggt
      601 ggtggagttc gggcgcaaaa tatttgcccc cgagtgcgag cgagaaggta tcgtcagcta
      661 cagcggctac cttggcttaa agcaagcggc gctctggctc gcactcttta taattgccgc
      721 ccaggtcttt ctcaccgttt gcctctccca tctggggcag ccgcttgcgg ccgggctggc
      781 atttgtcatt tatgacctgc tttgtttttc cgctcttttt ctaggtttta agaagggcgg
      841 tccgacagtt tcgaaagtgt ttgctgctct ctctaatatg tccgttctct ttggttacct
      901 ggtggtgctg ttgaactgca ccatgcattc gggagtgaat taaaaatgat tgctgagatt
      961 tattttgccg acggcgccaa ctgcgacatt tgcggcggta aggcacgggg actttcgaga
     1021 ctcaagcata taagcggtag cgccggcgcc agatttcagg ttccgccctt ttttgtgatc
     1081 gctgcggggg aagtggccgt ggcaaagttg caaggggcgc ttaccacaat taaagccgaa
     1141 tcgggctgcc ggagcttcgc cgtgcggtca tcttgtattg aggaggacgg tccgaccaat
     1201 tcatttgccg ggcagttcac atcttatttg tttgtagaag agtctcaggt gggactccat
     1261 gtagagaaag tacggcattc ggtcaaaagt aagtctattg agacgtacag gaagctcaag
     1321 ggtctcgatg gcagcggccc cgtggcggtt cttgtgcaag ccatggttga tgccagcgtt
     1381 tccggtgtca tgttcagcct cgatcctgtt acacagaaag cctgtcctgt aatctcaatg
     1441 gtggagggcg ccggcgacaa actgatggcc ggcaccgtca gcggtgagac cctttatatt
     1501 gccggtagta cgcctgctcc aggctcggcg cgccccagtt acttgactga agcaatgctg
     1561 gcgcagctct tgcgggccct tggcgccatc gaaaagcatt tcggtcgtcc catggatgta
     1621 gagtggtgtt tcgaccgaga ggggcgcctt ttcatcttgc aggccaggcc catcaccacg
     1681 gtggaccccc agagcaccgt attcgatgcc agtaatattc aggagagtta ccccggcaag
     1741 acttcacccc tgaccttctc atttgcccgc aagtgttata cggaagtcta taaaagtttt
     1801 gtcttgctca tgggggtgaa gccctctgtg gttgagagcc gcagcgatct gttcgagtat
     1861 atgatcggtt accacgaagg cagaatttat tacaacctgg gcaactggca tcgcttgatt
     1921 caacttttgc cgctttataa aagcaataaa ggttttatgg agcagatgat gggcttgaaa
     1981 gagcccctgc cccaggagcc gcaagcagcg ccctctaatt tcttcggtaa actcggcggg
     2041 ctcttttgcc tggcaaaaat tctctttgcc gcacttactt tgccgctact caaaaggtct
     2101 tttgaaaagc gtcttaacct ggccctcaac atcgataggc aggcggtcaa gaatctctct
     2161 ttgactgagc ttgcaagcct ttatttaagc ctggagagta ggcttatttc ccgctgggat
     2221 gcccctgtag taaacgattt cttcgccatg atcgcctacg gagccctgcg caagagtttg
     2281 agcggaaacc caaagaggat ggaatgcttg ccgttctatc ttaaatgtaa gagcgctgtt
     2341 gtgtcggggc agccgcccct gcttctgaaa gagctggccg ggcttatctc cgctgaccgc
     2401 gagcttatca ggcttatgga aagcggcaag accggtgctg ccagactgag gctcaaacaa
     2461 catccggaag ctgaaagact ctattgtctc tatctggaaa agtttgccga ccgcagtctt
     2521 ggtgaattga agttggaaag cgcctgcctg gaggatgatc cttccctact cttttccacc
     2581 ctggctgccc ttgctagagc tgaaaagaaa gctgcggtgg aaacgctgcc gcccccggca
     2641 ttggagcccc tgccctggcc tctagcgctt ctggcttctc ttacggcttc cttgctggcc
     2701 ggaagggaaa atctgcgttt tgcccgcacc cgggtctttg gcctggtgcg cctcattttc
     2761 aaaaccatgg gcaaaagact ggcggcctca ggacatattg agcagccgca ggatatttac
     2821 ttcctgaccg tagacgagat cctcggtttc atcggaggca cgggcgtcag cctcaaccta
     2881 gccgggcttg tgtccttgcg caaacgggag tatgaagggg cgagtgaatc gcccctgcga
     2941 ctcaccttca gcggtgccta cggcgatcgc aaggagttgc cgagagcggt gccggaggaa
     3001 gcggttaaag cttcccctca cacctcggtg ctggttggtc aggccgccag tgccggcatc
     3061 tgccgggggc gggtgcgcgt catcaggaac cctgaaagtg aagctctcca ggaaggcgag
     3121 atactggtag ccgaacgcac cgatcccggc tggattgtgc acttcagcct ggcggccgga
     3181 ctggtggtca gccatggcag cttgctttct catacggcca tcgtggccag ggaattatct
     3241 ctgccctgtg tggtggccgt ggagaacgcc accacccgcc tgaaaaccgg cgatctggtg
     3301 gaagtggacg gacgaaacgg caaggtcacc attttggaag aagcagtttc gaatctgttt
     3361 gcttgtgcct gaaaagtcaa tgccttgagc gcacaatgaa ggtgttgaga gcaacgagga
     3421 agagtggtat gagagccgga gccattgaaa gggtcaagtt gaacagcgcc ctgatcaggt
     3481 attcgcagtg ctgggaagac agtcaactt
//
LOCUS       DAOQNZ010000241         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_12983_length_3506_cov_3.215879, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000241 DAOQNZ010000000
VERSION     DAOQNZ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12983_length_3506_cov_3.215879"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PKW73_05585"
     CDS             complement(<1..1176)
                     /locus_tag="PKW73_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72786.1"
                     /translation="MSTAFLALLVSFGVPCRAQTADLSSEDLARNNSTIKRWLEKEKR
                     IFLNPISPDEVEVVLTRRTEPLCFLFIMGRYKQSGTNSAAQAEPKPPAAKVKEKDRLF
                     KEVQKASADLLKDSTRSVVLLDEKGEPPPPAYATELDEKSLNSAFTLISLGEYGRARK
                     LLQANQARLSDSMLINLAMLEAINGNIKKAEEILSKFSQDNPANATAQFNLAYLRLTQ
                     GKTKFAAALLKPLTEGESKGRLKLLSLLSLARLSLFEGKREEAVRYSERAHSDFPDSL
                     DSLDTAGDIALACKDFKKAIQYLTPVAQARKSETRYLLKLAEAHQELGETDRAIRLLN
                     QACDIEPESVANHVHLGKTYLANKDFLAARLQFERALELKPDLDEKRKIFAPFIKVLN
                     "
     gene            1291..2487
                     /gene="tgt"
                     /locus_tag="PKW73_05590"
     CDS             1291..2487
                     /gene="tgt"
                     /locus_tag="PKW73_05590"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNA72787.1"
                     /translation="MATSSNSSNNPAQTTVSSVSKFDSPALSYEVQAVCPWSGARAGL
                     LTTPHGVVETPVFMPVGTNAALRALTWEQTADCGAQIVLSNSYHLYLRPGHELIKEAG
                     GLHKFMHWDKPILTDSGGFQVFSLDALRKITEEGVYFKDHKGGKTHFIGPKESMTIQN
                     AIGPDIMMAFDECVKNPATEEEARAAMERTHRWLAVCQQEHKRKHDQSLFGIVQGSMY
                     EHLREESVQAVTAYDLPGYAVGGVAVGEDRRTIERIVLHTTPLLPREKPRYLMGVGTP
                     WDLVYAIRCGIDMFDCVSPTRLARHGTVFSSRGRLAVKNAGFRKDFGPIDETCPCSTC
                     KNHSLAYLHHLVRQGEMAGLMLLAIHNVTFLIEEAQNCRKAILSGTFEDYFSRWSSAR
                     GPLEPQ"
     gene            complement(2444..3217)
                     /locus_tag="PKW73_05595"
     CDS             complement(2444..3217)
                     /locus_tag="PKW73_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNA72788.1"
                     /translation="MLDRLVQVIKAAFNALLGKVEDPQMMLEQSYNELQSNVISVRQA
                     VAQAIATEKQLERELQKNKDQAATWQNRAAMAVQQNSDELARQALQRKQQYAQAAADL
                     ETQLKTQKEATAGLRQRLGELENELQKANTKKQVLIARDKAAKATIKANEILSKTNTS
                     GALSVMERMESKVQEKEAKALALGELSGDSLEKQFKSFEGKSDIELELLALKGEMGKT
                     AEPKLINTDKQDAVLLERQLDNVDSSIEVQEVRELKTND"
BASE COUNT          736 a          929 c          933 g          908 t
ORIGIN      
        1 gttcagcacc tttatgaatg gtgcaaatat tttccttttt tcatcaagat caggcttcaa
       61 ttccagtgct cgttcgaact gcaagcgggc tgccaggaag tccttattgg caagataggt
      121 cttgcctaga tgcacgtgat tagccacgct ttccggttca atatcgcagg cctgattgag
      181 aagtctgata gccctatccg tctcgccaag ttcctggtgc gcctctgcca acttaagcag
      241 gtagcgggtt tccgacttgc gggcctgggc taccggagtc aggtactgaa tagctttctt
      301 gaaatctttg caggccaggg cgatatctcc ggcggtatca aggctgtcga gactatcggg
      361 aaaatcggag tgcgctcgtt ctgaataacg caccgcctcc tcgcgcttgc cctcgaaaag
      421 agaaagcctg gccaggctca gtaagctaag gagcttcagt cggcctttgc tttccccctc
      481 cgtgagaggc ttcaagaggg cggcggcaaa cttggtttta ccctgggtga ggcgcaagta
      541 ggccagattg aactgggcag ttgcattagc cggattgtcc tggctgaact tagagagaat
      601 ctcttccgcc ttttttatat tgccgttaat ggcttccaac atggccagat taataagcat
      661 agaatctgaa agcctggctt gattggcttg tagaagtttg cgcgctcggc cgtactctcc
      721 caggctgatc aatgtaaagg cgctatttaa gctcttctca tccaattcgg tagcataggc
      781 gggagggggc ggctctcctt tttcatcgag aagcacgaca ctgcgggtgg agtcttttaa
      841 gagatctgct gaagcctttt gcacttcctt gaagaggcgg tccttctctt ttacttttgc
      901 cgcaggcggt ttgggttcag cctgggctgc cgaattcgtc cctgattgtt tatagcggcc
      961 catgatgaat agaaaacaaa gtggctcagt tcgcctggtt agcactactt ccacctcatc
     1021 cggggaaata ggattgagaa agatgcgctt ctccttttcc agccagcgct taatggtgga
     1081 attgttacga gccaggtctt ccgaacttaa gtcggcggtt tgagcccggc agggcacgcc
     1141 gaagctcact aaaagggcaa ggaaagccgt gctcaagctt ccatgtctag atcttctggt
     1201 gtgggtcttc atgtgcggca ttattacaca ggtgcttgat agcgcgcccg ggaattactc
     1261 tttgggcctc attcgctata atcagagctt atggcaacct caagcaatag ctctaataat
     1321 ccggctcaga ccacggtcag ctcggtgagc aaattcgatt ctcccgctct ttcctatgaa
     1381 gttcaggcgg tttgtccctg gtccggggcc agggccggac tgctcactac tccccatggg
     1441 gtggtggaaa cgcctgtttt catgccggtc ggcaccaatg ccgccttgcg tgctctcacc
     1501 tgggagcaga cggccgattg cggcgcccag attgtgcttt ccaattccta tcatctttac
     1561 ctgagacccg gtcacgaact tatcaaagaa gcaggcggac tgcataagtt tatgcactgg
     1621 gataagccca tcttgaccga ttccggcggt ttccaggttt tcagcctgga cgccctgcgc
     1681 aagataaccg aagagggggt ctactttaaa gaccacaagg gcggaaagac gcactttatc
     1741 ggaccgaaag agtctatgac catccagaac gccatcggcc cggacatcat gatggccttt
     1801 gacgaatgtg tgaagaatcc ggccaccgaa gaagaggccc gtgcggccat ggagcgcacc
     1861 caccgctggc ttgcggtttg ccagcaggaa cataagcgca agcacgacca gtctctattc
     1921 ggcatcgtcc agggcagcat gtatgagcac ctgcgggaag aatcggtgca ggccgtcact
     1981 gcctatgatc tgccgggcta tgccgtgggc ggtgtggcgg tgggggaaga ccggcgcacc
     2041 atcgagcgta ttgtgctcca caccactccc cttctgcccc gggaaaagcc ccgttactta
     2101 atgggagtag gtaccccctg ggatctggta tatgccatca gatgtggtat cgatatgttc
     2161 gactgcgtgt ctcccacaag gctggccagg catggcacgg tcttttccag caggggacga
     2221 ctggctgtga agaatgccgg tttccgcaaa gatttcggac ccatagacga gacctgccct
     2281 tgctccacct gcaagaatca cagcctggct tatctccatc atctggttcg tcagggagaa
     2341 atggccggtc taatgctttt agccatacac aatgtcacct tcctcattga ggaggcgcaa
     2401 aattgtcgca aagcaatcct ctcgggtact ttcgaggatt acttcagtcg ttggtcttca
     2461 gctcgcggac ctcttgaacc tcaatagagg aatcgacatt atctagctga cgctccagaa
     2521 gcacggcgtc ctgcttgtcg gtattgatta gcttgggttc ggctgttttg cccatttcgc
     2581 ccttgagggc aagtaattcc agttcgatat cgcttttgcc ttcaaagctc ttaaactgct
     2641 tctccaggga gtctcccgat agttctccca gggcaagggc ctttgcttcc ttctcctgca
     2701 ccttggattc catccgctcc attactgaaa gagcgccgga ggtattggtt ttggagagaa
     2761 tctcattggc tttgatggtg gctttagccg ccttatcgcg ggcaatgagc acttgcttct
     2821 tggtattggc tttctgcagc tcattttcca attcacccag gcgctgcctg agaccggccg
     2881 tagcctcttt ctgggtcttg agttgggtct ccaggtcggc tgccgcctgg gcatactgtt
     2941 gttttctctg cagggcttgc ctggccagct catcgctgtt ctgctgcacg gccatggcgg
     3001 cgcggttctg ccaggtggcg gcctggtctt tattcttttg taactcgcgc tccaactgct
     3061 tctcagtggc aatggcttga gctacggcct gtctcactga aattacgttg ctttgcaact
     3121 cgttgtatga ctgctcgagc atcatctgcg ggtcttcaac cttacctaaa agggcgttga
     3181 atgcagcctt gatgacttgt actaagcggt ccagcatctg agagaaactc cttccattaa
     3241 ggacgggcga tttaatcaat atctagtcat agcatgaatt tgctcttaac atgatagtag
     3301 acaaacctgg ttgtaactct tatgcccgct ttttacatcg tcaaccccgc ccgacgggga
     3361 taaatcccac tctaagttga agacattatg aagaatgaaa gctccaagaa cgacagaagc
     3421 gaaggcggca gcctcggcgc ccagattatg atggcttctg aaatgggggc ggccatagtc
     3481 tttttgagcc tggccggcta cctggg
//
LOCUS       DAOQNZ010000242         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13064_length_3494_cov_2.576912, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000242 DAOQNZ010000000
VERSION     DAOQNZ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13064_length_3494_cov_2.576912"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKW73_05600"
     CDS             complement(<1..398)
                     /locus_tag="PKW73_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72789.1"
                     /translation="MKLAKALSGILLLAALQGASANAPSFAEWYDSDRSCLERARTLF
                     GTGQFDEAQKALDKGISLNPRNAELFYMRGVLYSTLKRFDLALQDMDQALQLNPNSAN
                     YFIARGTLYSNMTKYDLAIKELKKAYLLKR"
     gene            complement(622..1281)
                     /locus_tag="PKW73_05605"
     CDS             complement(622..1281)
                     /locus_tag="PKW73_05605"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNA72790.1"
                     /translation="MNLLEKLPVALSGLFFAALCNGPALAQEVDLPDGVADGPPPMEG
                     MVEISLDSDGDPEFTLAMDPNTIQMGAPGGPPPRMMMRRGWGGRSAMGCGGGGGVGCP
                     VLGALDLTDDQYEKFYALKNSFLDKVGPKMLEIRTHERHLRDLMTQTNVDAKAARNLQ
                     DKINGLKADLANMKLDNRLAMHDVLNEEQRKKIRDWVVKGGRGMHHRGGPRMKMEKGD
                     G"
     gene            1557..2948
                     /locus_tag="PKW73_05610"
     CDS             1557..2948
                     /locus_tag="PKW73_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA72791.1"
                     /translation="MMRGTQEKPVGTMENSGSTAQGNPKVSPASAKVGNYRWFIIALG
                     FIITLINYLDRSALAYTRKALIGEFGINDADFGMILSAFAITYMVMTTVGGIMVDKWG
                     AHKVWPTAAILWSAVTAMMGLASGMWVLFAFRLFLGVVEGPHFPALTRAVADWLPTNE
                     RARATAIGLAAVPFAHVVGGPLITSLIGSLGWKGMFVVLGALGVVWSVAWVFLFRDYP
                     ESCPFVSDAELNHIREGKSQNREISDVERRSHHLAAGKTTWKFMLLNPSLMSNNIAFF
                     SFGYLLFFSMNWLPSYLEKTYHMNLHQVGWFVVAPWLTAAILLAAAGFVSDLVMRKTG
                     SARLARTHMIWVCQLISALSFIPLMFTQDLNVAMVMMSLGIGIGLMPNSCFYALNTDL
                     AKDRAATSLGIMDSFFALGGILAPLVTGQILQRTGSFQLAIGLVVFFSLMSVIGIILF
                     QKPDRDMEAVKGA"
     gene            3092..>3494
                     /locus_tag="PKW73_05615"
     CDS             3092..>3494
                     /locus_tag="PKW73_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72792.1"
                     /translation="MPASKTELKAALLAVSLIFSLPPAAFSEETTEAISQTADLVEKT
                     NLEELGFKEYKPAKTEQSAKPAAKPIWVQKADAASKTDCAKKQSSVPVNSDQKADAGQ
                     KADFVGKAAWYSSDLHGKKTASGQPYDEGKLT"
BASE COUNT          831 a          926 c          902 g          835 t
ORIGIN      
        1 atgcgcttga gcaggtaggc cttcttcagc tctttgatgg ccaggtcgta cttggtcatg
       61 ttggaataaa gggtgccgcg cgcaatgaaa taattggcgg aattgggatt gagctgaaga
      121 gcctgatcca tgtcttgcag agccagatca aaacgcttca gcgtgctgta gagaacgcca
      181 cgcatgtaga acagttcagc gttgcgaggg ttgagagaaa ttcctttatc gagggctttc
      241 tgcgcctcgt caaattgacc ggtaccgaaa agcgtgcgag ctctctccag gcacgaacga
      301 tcgctgtcgt accattccgc aaaactcggc gcattcgccg atgctccctg caaagccgcc
      361 aggagcagaa ttccgcttag tgcttttgct aacttcatat caacgcacca ttagtaaatg
      421 aggggtgggt ggcgaattaa aagcgcgtgg gcctttaact gaaatattaa tacgtccctg
      481 ccgttgaaaa ttaggggagg aacgatagat taagcagatt gccaacaaaa gagggagtcg
      541 caaaagactc cctctctgtg aaaactccga aggactggga gttttcggtg tttgctccga
      601 gatcaggcgg acggcgactg cctaaccatc gcccttttcc atcttcatcc ggggcccgcc
      661 gcggtgatgc attccgcgcc cacccttaac gacccagtcg cgaatttttt tcctctgctc
      721 ttcattaagg acatcatgca ttgccaggcg attgtcaagc ttcatattgg ccaggtcggc
      781 tttcagtccg tttattttgt cctgtagatt acgagctgct ttggcatcta cattcgtctg
      841 agtcatcaaa tctctcagat gcctttcatg ggttcggatt tccagcatct tgggcccgac
      901 cttgtccagg aaagaattct tgagagcgta gaacttctcg tactggtcat cggtcaggtc
      961 gagcgctccc aagacggggc aaccgactcc gccgcctccg ccgcagccca tggcgctgcg
     1021 tccgccccag ccgcggcgca tcatcatacg ggggggcgga ccaccagggg cacccatctg
     1081 gatagtgtta gggtccatag ccagagtaaa ttcagggtcg ccatcagagt cgaggctgat
     1141 ttccaccatt ccttccatag gaggcggtcc gtctgcaacg ccatcaggca gatcaacctc
     1201 ctgcgccaat gccgggccat tgcacaatgc cgcaaagaac aagccggata gggcgaccgg
     1261 cagtttttcc aatagattca tttagtactt ctcctggaga tatcccgaaa agggaggcct
     1321 gactgggcca ttactaggta aagacgagat ttgccggaaa aaagttcaaa gccgaagata
     1381 aaatctaaca aatagctcgc ccgctgatat cttcttcccc ttcaagctgc caacaagaaa
     1441 cggtttatcc gttaaacttc tcagttcaat caccgagtga ttttgctttt cctctgccga
     1501 gccaggcccg gcaatcggag caaaattcgt gtccgactgc tggaagccca ggaggcatga
     1561 tgagaggaac ccaggaaaaa ccggtaggca ccatggaaaa cagcggttca accgcacaag
     1621 gaaacccgaa ggtcagtcca gcaagcgcta aagtcggcaa ctatcgctgg tttatcatcg
     1681 ctctgggttt cataatcact ctcattaact atttggaccg ctccgccctt gcttatacgc
     1741 gcaaggctct gattggagaa ttcggcatca acgacgctga tttcggaatg attctgtccg
     1801 cctttgcaat cacttatatg gtgatgacta cagtgggcgg catcatggtc gataaatggg
     1861 gtgctcacaa agtgtggccg acagccgcca ttctctggtc ggcagtgacg gcgatgatgg
     1921 gtctggcgtc aggaatgtgg gtcctgtttg ccttccggct ctttcttgga gtcgtcgaag
     1981 gtcctcactt tcccgctctc acacgcgccg tggccgactg gttgcccacc aatgagcgcg
     2041 ctcgagccac cgccatcggt cttgccgccg ttcccttcgc gcacgtagtg ggcgggccgt
     2101 tgattacatc cttgataggc agtctcggct ggaaaggaat gttcgtcgta ctgggcgccc
     2161 tgggcgtagt ctggtcggtc gcctgggttt tcctcttccg tgattatcct gagtcttgcc
     2221 cgttcgtttc cgatgcagag cttaaccata tacgcgaggg caaatcgcaa aatagagaaa
     2281 tcagcgatgt agagagaaga tcgcatcatc ttgccgccgg caagaccacc tggaaattca
     2341 tgcttctgaa tccgtcgctg atgtccaata acatcgcctt cttctcattc ggttatctgc
     2401 tcttcttttc gatgaactgg ctgcccagtt atctggaaaa gacctaccat atgaatcttc
     2461 accaggtggg ctggtttgtc gtagcgccct ggctgacggc agctattttg ttggcagcag
     2521 ccggcttcgt ttccgacctg gtaatgagaa agacaggaag cgcccgtctt gctcgcacgc
     2581 acatgatctg ggtttgccag ctgatttcgg cgctcagctt cattcctcta atgtttacgc
     2641 aggatctcaa cgtagccatg gtgatgatgt ccctgggcat cggcattggc ttgatgccga
     2701 attcatgctt ctatgccctt aacacagacc tggcaaaaga cagggctgcg accagccttg
     2761 gcattatgga cagcttcttc gccctgggcg gaattcttgc ccctctggtc actggtcaaa
     2821 tcctgcaacg caccggcagc tttcaacttg ctatcggtct ggtcgtgttt ttcagcttga
     2881 tgtcggtgat cggcataatc ctgttccaga agccggatcg agacatggaa gctgtcaagg
     2941 gcgcctaatc tctaatgcaa gttcttggaa attagggaca attctcaagg aagtctcgaa
     3001 cgccgaaata tatcaattcg tcaccctttc ctgctgcgct ccggcgccat tgcaaaggga
     3061 ggtcgtttgg atgctactaa gggagattgc aatgcccgcc tcgaaaacag aattaaaggc
     3121 agcactactg gcagtatcac tgattttctc tctcccccct gcagctttta gtgaagaaac
     3181 aactgaagct atctcgcaaa ctgcagatct tgtagagaag acaaatctcg aagaactggg
     3241 cttcaaagaa tacaaaccag ccaagacaga acaaagcgcc aagccggcgg caaagcctat
     3301 ctgggtgcaa aaggccgatg cagcgagcaa aacagactgt gcaaaaaaac aatcctcggt
     3361 gccagtaaat tccgatcaga aagcagatgc tggtcaaaaa gcggacttcg taggcaaagc
     3421 agcctggtat agcagcgatt tgcatggaaa gaaaacggca tcgggacagc cttatgacga
     3481 aggtaaactg actg
//
LOCUS       DAOQNZ010000243         3476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13180_length_3476_cov_3.639287, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000243 DAOQNZ010000000
VERSION     DAOQNZ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13180_length_3476_cov_3.639287"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="PKW73_05620"
     CDS             <1..1660
                     /locus_tag="PKW73_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA72793.1"
                     /translation="QKNTANHGSGVGILLEFRGLIEVIEVQGEWQKPLEKLSQELDDL
                     RKTLKSIDESKDQLRPELKRATGEVATLVKSKDSHIDPLYELPRILTAILENPKIGAL
                     VFGTRGERLLYNKRAQDLLGNLIDSTECGGEGARQEHHSGFFTPGQEERPLQAGELPW
                     NFTNTDGGAGQRESELLVKHPGQENVWLRVTVSPLSSSQPGKEPGGVIAFLADITEHV
                     KVVNELSNICRDTEKRLDEFSKGIEDLANLANILAKAKKTEAPQQERQTTEVSGETSK
                     PASLGAGLKVLVVDDVAVNQKLLKLQLEKLGFAVELAGDGREACEKVKDSKPAFDLIL
                     MDCDMPKMDGYEATQLIRAQEGEKAVPIIAVTAYDREGDREKCLEAGMSDYITKGSPE
                     KELQKLLSKWLKRETGEAEEEGKSGSIARSLLFDEGEILGENFKQNQLDFAELSRIYG
                     QEEALEIIKLFLGVTGTFTECIDLALTSKDIDAVNHFAYSIKGPFSSLQLTALSDLAS
                     KLPKLAAKHKWKDAKKVYGQLLELYKPIKSQMEEEIQKSTSTKN"
     gene            1672..>3476
                     /locus_tag="PKW73_05625"
     CDS             1672..>3476
                     /locus_tag="PKW73_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA72794.1"
                     /translation="MPQDEEFLKRLRAAFLEEGGEILAEISKRLADLENTADFPEQVT
                     LLGKVAAQLHSLKGSARAVNEDDLVGICQGLETVFLHLKKTDQDNLPRHLLVAQPVLF
                     EIVDTMNEFFDTQAGITSEGLTPVEKSGLKAAVDIKLSMLNHRLGKSEAQVPEPPAKP
                     VSKPVQEVRQTSEQMQTGTFNVTELRAMMLPLESETGERAKNAAAKTNPARGDGNETV
                     RVPVDRLDRLLTESEELLLLKGQMQDRLGLISNLRLLLEDMAREKQSQPAGAELKEAR
                     RQVNQIYRLLLKDTSNASHMLTRFLDTAKNLTMQPMNSTLEVFPRLVRDLARELRKEA
                     ELQIDGGHLEIDRRILEKIKDPLTHVLRNALDHGIESPEAREQSGKPRKARLKLAVKQ
                     LSVENLEITLEDDGAGVNAERVKAKAVEKGIISQEEAKNLSYGQALGLIFRPDFSTRD
                     EVSELSGRGLGLAIVKEKIEELSGRLEIVSTEGSGTTFKIVLPTKLAAFSGIKVQSQG
                     QFFVIPVAGLVRAMRVHPLAMEITGGRRTFFVDGKLIPVATLAKTLSIPEKDSGAERS
                     THFRYLELLVLQSREKTAGLIVDQVLDQGEFLVKKL"
BASE COUNT         1001 a          903 c          938 g          634 t
ORIGIN      
        1 ccagaaaaat accgccaacc atgggagcgg ggtcggcatt ttgttagaat tcagaggtct
       61 catagaggtg atagaagtgc aaggtgaatg gcagaaacca ctggaaaagc tctcgcaaga
      121 actggacgac ttgcgcaaga cgcttaaatc gattgatgaa agcaaagacc agttgcgccc
      181 cgagctgaag cgagccacag gcgaggtggc caccctggtc aaaagtaagg acagccatat
      241 tgaccctctt tacgagctac ccagaatatt gacggcgatc ctcgaaaatc cgaaaatcgg
      301 tgccctggtc ttcggcacca ggggcgaaag attgctctat aacaaacggg ctcaggactt
      361 actgggcaac ctgattgaca gtaccgaatg cggtggcgag ggcgccaggc aagagcacca
      421 ctccggcttc tttactcccg gtcaggaaga gcgccccttg caggcgggag aactgccctg
      481 gaatttcacc aacaccgatg gcggtgccgg gcaaagagaa tcggaattgc tggtcaaaca
      541 cccggggcag gagaacgtct ggctgagagt caccgtcagc cctctttcaa gcagtcaacc
      601 aggtaaagag cctggtggtg tgatcgcctt cctggccgat atcacggagc acgtcaaggt
      661 agtaaacgaa ctcagcaaca tatgccgcga tacggaaaag agactggatg aattcagtaa
      721 aggcatcgaa gacttagcca acctggcaaa tatactggcc aaggcaaaaa agactgaagc
      781 cccgcaacag gaaaggcaga cgaccgaggt ctccggcgag acctccaaac ctgccagcct
      841 gggtgcgggg ctaaaagttt tggtggtaga tgatgttgcc gtcaaccaga agctgctcaa
      901 gctccagctc gaaaaactgg gtttcgccgt agaactggcc ggcgacggca gagaagcctg
      961 tgagaaagtc aaggacagta agcctgcctt cgacttaatc ttgatggact gcgacatgcc
     1021 aaaaatggac ggctacgagg ccacccaatt aatccgggcc caggagggag agaaagcagt
     1081 tcccatcata gcggtcaccg cttacgaccg ggaaggcgat cgggaaaagt gcctggaagc
     1141 cggcatgagc gactacatca ccaaaggctc tcccgaaaaa gagctgcaaa aactgctgag
     1201 caaatggttg aagcgggaaa ccggggaagc tgaagaagaa ggcaaatcag gcagtattgc
     1261 ccgttcactc ttgtttgacg aaggtgaaat actgggcgag aacttcaaac aaaaccagct
     1321 cgattttgcc gagctttcca gaatatacgg acaagaggaa gccctcgaaa tcatcaagct
     1381 ttttctggga gtgaccggca catttaccga gtgcatcgat ctcgcgctca cctccaaaga
     1441 catcgacgcc gtcaatcact ttgcctactc catcaagggt cctttttcct ccctgcaact
     1501 gactgcccta tcggacctgg catcaaaact gcccaaacta gcggctaagc acaaatggaa
     1561 agacgccaag aaggtctacg gacaattact ggagctctac aaaccgatca agagtcagat
     1621 ggaagaagag attcagaaga gcacctcgac taagaactga gttttccaac tttgccacaa
     1681 gacgaagagt ttctaaagcg cctgagagca gcctttcttg aagagggtgg agaaattctg
     1741 gcggaaataa gcaagcgtct tgcagacttg gaaaatacgg cagactttcc tgagcaagtg
     1801 actttgctcg gcaaggtagc agcgcaacta cacagcctca aaggttcagc ccgggcagta
     1861 aacgaagacg acctggtagg catctgccag ggacttgaga cggtctttct gcacctcaag
     1921 aaaaccgatc aagataatct gccgcgccat cttctggtag cgcagccggt gctcttcgaa
     1981 attgtggata ccatgaacga gttcttcgac actcaagccg gtatcacatc agaagggctc
     2041 actcccgtgg aaaagagcgg cttaaaagca gccgtagata ttaagttgag catgctcaat
     2101 catcgcctcg gcaaaagtga agcacaagta ccggagccac cggcaaagcc agtaagtaag
     2161 ccggtgcagg aggtcaggca aacatcggag caaatgcaaa ccggcacctt caacgtcacc
     2221 gaactaaggg ccatgatgct gcctctggaa tcggaaaccg gcgagagagc caaaaatgcc
     2281 gccgccaaaa ccaacccggc aaggggcgac ggcaacgaga ccgtgcgtgt gccggtggac
     2341 aggctggata gattgctcac cgagtcggaa gagctactgc ttttgaaggg acaaatgcag
     2401 gaccgcctcg gtcttatctc aaacctgcga ctcctgctgg aagatatggc cagagaaaag
     2461 caaagccagc cggccggtgc cgagctaaaa gaagcacgcc gccaggtaaa ccagatatac
     2521 aggctcctgc tcaaggatac ctccaacgcc tcccacatgc tgacacgctt tctggacaca
     2581 gccaagaatc tcaccatgca gccgatgaac tcgaccctgg aggtttttcc aaggctggta
     2641 agagatctgg cgcgggaact tagaaaggaa gcggagctac aaatcgatgg cggtcatcta
     2701 gaaatagaca gacgtatact cgaaaagatc aaggatcctc tcacccatgt actgcgcaat
     2761 gccctcgacc atggcatcga aagccccgag gcgcgagaac agtcagggaa gccccgcaag
     2821 gccaggctaa aactagccgt caagcaactt tcagtagaaa atctggaaat caccctggaa
     2881 gatgacggcg ccggcgtcaa tgccgaaagg gtgaaagcca aggccgtcga gaagggtata
     2941 atcagccagg aagaagctaa aaatctctct tacggtcagg ccctcggtct gattttccgc
     3001 cctgattttt ccacaagaga cgaggtatca gaactatcgg gacgcgggct gggtctggcc
     3061 atagtgaaag agaaaatcga agagctctcc ggtcgcctgg aaattgtcag cactgaaggt
     3121 agcggcacca ccttcaagat cgtcttaccc actaaactgg cggccttcag cggcatcaaa
     3181 gtgcagagcc agggacaatt cttcgtcata cctgtggctg gtctggtcag ggccatgcgc
     3241 gtgcatccac tggccatgga gataaccggc ggaagacgta ctttctttgt ggacggtaaa
     3301 ctgataccgg tagccaccct ggctaaaaca ctttccattc cggaaaaaga ctcaggcgcg
     3361 gaacgctcca cgcactttcg ttatcttgag ctattggtct tgcaatccag agagaaaaca
     3421 gcaggtctga tagttgatca ggtgctggat caaggagagt ttctcgtcaa gaaact
//
LOCUS       DAOQNZ010000244         3474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13195_length_3474_cov_4.795262, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000244 DAOQNZ010000000
VERSION     DAOQNZ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13195_length_3474_cov_4.795262"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PKW73_05630"
     CDS             complement(<1..828)
                     /locus_tag="PKW73_05630"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HNA72795.1"
                     /translation="MKGLILSGGKGTRMRPITHTAAKQLLPVANKPILFYGIEALIDA
                     GIKEIGIIISPETGNDVKECVGDGSRWGIKIDYILQAEPLGLAHAVKTARPFLQDSPF
                     VMYLGDNLIKDGVGPLVERFKEGGADAFILLKEVANPSSFGVALLDPDGRINCLEEKP
                     AKPKSNLALVGVYLFNPKIHVAIDQIKPSRRGELEITDAIQQLISTGNRVDSHILTSW
                     WLDTGKKDDMLEANRVVLDEMTEVSMNGDMDDSSRVSGRVQIGKGSKLVNCTVRGPAV
                     "
     gene            complement(936..1577)
                     /locus_tag="PKW73_05635"
     CDS             complement(936..1577)
                     /locus_tag="PKW73_05635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNA72796.1"
                     /translation="MNDHLLKYATDASRLRNEAQKSKVEAMLAKRLTDIFISVSALLG
                     FAPLLAIIAVLIKLDSNGPVIFKQRRVGQDGEFFEIYKFRTMRIGTPDLPTDQMLKLE
                     SPITRVGSFLRKTSLDELPQLFNVLAGQMSLVGPRPALYNQVELTEKRKSEGVLRFPP
                     GITGWAQVNGRDELPDDVKVKADKWYCEHWNYWLDWRIIFLTFGAVFSKKGAN"
     gene            1648..2535
                     /locus_tag="PKW73_05640"
     CDS             1648..2535
                     /locus_tag="PKW73_05640"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA72797.1"
                     /translation="MLLSVVIVSWNTRDLLRACLVSLKEEIATMPNPADVEVFLVDNA
                     SADGSAKMAQEEHPWVHLIANSDNKGFAKANNQAFALAGGRFILLLNPDTEIKPGALS
                     TLLAFFDKHPRAGIVAPQLLNTDGTVQRSCREFPTFVGMLYELIGLSRLFPANSSYGK
                     EFRRYKMLDWNHDDERQVDQPEGACLLVKREVLEKVGTLDEGFFMLFEEVDWCFRIIK
                     AGYEIWFTPRAQVVHHYGQSIKQVKVPMILSSHRGLYRFWYKHYRGERWWLDGFAYIS
                     LMSLAYLRIASYRLRSLFS"
     gene            2710..>3474
                     /locus_tag="PKW73_05645"
     CDS             2710..>3474
                     /locus_tag="PKW73_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72798.1"
                     /translation="MLFTSLQYLVFLPVIVLLFWVLPRGWRLPMLLVASYYFYASFIW
                     QFLALILPMTLFNWLWGKLLVKDHSNKKILGVGIAVNLFILAVFKYANFFADSLGAIF
                     PLVGAANPHWTFNIILPLGISFFTFEFIHYLFEIYRGKKPIDSFVLFALFAAFFPTQI
                     AGPIKRYPDFVAQMQADNKLKLSDFDEGVPLIIIGLAKKLLLADNLSIFVQMGMDHPE
                     LYSAPELWLFAYAFAFQIYFDFSGYTDVGRGSALLFG"
BASE COUNT          722 a          889 c          879 g          984 t
ORIGIN      
        1 tactgccggt ccacgcacgg tgcagttgac cagtttgctg cccttgccga tttgcacgcg
       61 gccggaaact ctggaactgt cgtccatgtc tccgttcatg gaaacttcgg tcatctcgtc
      121 caggaccacg cggttggctt ccagcatgtc gtcttttttc ccggtgtcca gccaccagga
      181 agtaagaatg tggctgtcta cccgattgcc ggtggaaatc aattgctgaa tggcgtcggt
      241 aatttccagt tcgccacggc ggctgggctt gatttgatcg atggcaacat gaatcttagg
      301 attaaagagg taaaccccaa ccagggccag gttgctcttt ggcttggccg gtttttcttc
      361 caggcaatta atgcgtccgt ccggatcaag gagagcaacg ccgaaggaac tgggattggc
      421 tacttctttg aggaggatga aggcatcggc gccgccctct ttgaagcgct ccaccagcgg
      481 tccgacgccg tctttgatga ggttgtcgcc caggtacatg acgaagggcg aatcttgcag
      541 gaaggggcgg gcggtcttaa ctgcatgcgc cagtccgagg ggttccgctt gcaggatgta
      601 gtcgattttg atgccccagc ggctgccgtc gccaacgcat tctttgacat cgttgccggt
      661 ttcggggctg atgatgatgc ctatctcttt gatgccggcg tcaatcaggg cttctatacc
      721 gtaaaacagg ataggtttgt tagccacagg cagaagctgt ttggcggctg tatgggtgat
      781 ggggcgcatt cttgtgcctt ttccgccgct tagaattaaa cctttcattt cgtctctcct
      841 gggcttgtcc atttatctgt gcgctattag ctggttgctc taaagttgtc tcaaaactca
      901 aaaatgttta acttgggttt aaggcttgat ctgtctcagt ttgcaccctt tttactgaag
      961 acggcgccga aggtcaggaa gatgatgcgc caatcgagcc agtaattcca gtgctcacag
     1021 taccacttgt ccgctttcac tttcacatcg tcgggtagtt cgtcgcggcc gttcacttga
     1081 gcccagcctg tgatgccggg tgggaaacgc aggacacctt cgcttttgcg tttttccgtc
     1141 agttcgactt gattatacag agccggtctg ggtcctacta aactcatctg ccccgccagt
     1201 acgttgaaaa gctgcggaag ctcatccaga ctggtctttc gcagaaagct gccgaccctg
     1261 gtaatggggc tttccagctt caacatctgg tctgtaggca ggtccggtgt gccgattcgc
     1321 attgtgcgga atttataaat ttcaaagaat tcgccatcct gaccgactcg ccgctgcttg
     1381 aagataacgg gaccgtttga gtcgagtttg atcaaaacgg caatgattgc cagaagcggg
     1441 gcaaatccca gcagggcgga aaccgagatg aaaatatcgg tcagtctctt ggcaagcatg
     1501 gcttcgactt tactcttttg agcttcgttt ctgagtcttg aggcgtcagt cgcgtatttt
     1561 agaagatggt cgttcatcct ttcctgagcg ccgttataag attgccctaa attatttggg
     1621 caccaggcaa gctacacttt tcgttctatg cttttatctg ttgtcatcgt tagctggaat
     1681 accagagatc tcctgcgtgc ttgcctggtt tccctcaagg aagagattgc cacaatgccc
     1741 aatccggcag atgtggaagt ctttctggtg gacaacgctt ccgccgatgg ttcggcgaaa
     1801 atggctcagg aggaacatcc ctgggtgcat ctgatcgcca atagcgacaa caaaggtttc
     1861 gccaaagcta ataatcaggc ctttgccctg gccggcggcc gcttcatcct tttgctcaat
     1921 cctgataccg aaatcaagcc cggtgcgctt tctacactgt tggctttctt cgataagcat
     1981 ccccgggccg gcattgtggc gccccaattg ctcaataccg atggcaccgt acagcgctcc
     2041 tgtcgcgagt ttccgacctt cgtgggcatg ctctacgaat tgatcggact ttcacgcttg
     2101 tttccggcca acagcagcta cggcaaagag tttcgccgct acaagatgct tgattggaat
     2161 cacgatgatg aaaggcaggt agatcagccg gaaggggctt gccttctggt taagagagaa
     2221 gtactagaga aggtgggcac ccttgatgaa ggctttttta tgctcttcga ggaagtggac
     2281 tggtgtttcc gcatcatcaa agccggctat gaaatctggt ttactccaag ggctcaggtg
     2341 gtgcaccatt acggtcaatc catcaagcaa gtgaaggtgc cgatgattct ctcgtcccat
     2401 cgaggtctct accgtttctg gtacaagcat tatcgtggtg agcgctggtg gctggacggt
     2461 ttcgcctata tttccttgat gagtctggct tatttgcgca ttgcatcata tagattgcgt
     2521 agtcttttct cctgataaga gacgacaagt cctatgaaaa aagatttaga gattgtttct
     2581 ctcatcaggt caactgctgc ttctattaaa ctggaatagg acttgaaaag aggatgaatg
     2641 atagaatcgc agcgtttcat ctcttgataa tttgataaac acctgtccag tccaactcgg
     2701 ggaaaccaga tgctgttcac cagccttcag tatttagtat ttttgccggt gatagtactt
     2761 ctgttctggg ttctgccgcg gggctggcgt ttgcctatgc tgcttgtggc cagttactac
     2821 ttttatgcca gttttatctg gcaattcctg gctctcatcc tgcccatgac cctcttcaac
     2881 tggctgtggg gcaagctctt agtcaaagac cacagcaaca agaagattct gggtgtaggc
     2941 attgccgtca acctttttat tctggccgtc ttcaagtatg ccaacttctt tgccgattct
     3001 ctgggcgcca tctttcccct ggtgggtgcg gcgaacccgc attggacctt caacataatc
     3061 ttgcctctgg gcatttcttt ctttactttc gagttcatcc attacttatt tgagatttac
     3121 cgcggcaaaa agcctatcga ttcttttgtt ctctttgctc tctttgctgc cttcttcccc
     3181 acccagattg caggtccgat caagcgctat cctgatttcg tggcgcaaat gcaggccgac
     3241 aacaagctca aactctccga cttcgatgaa ggggtgccgc tcatcatcat cggtctggca
     3301 aagaaattgc ttctggccga caacctgtcc atctttgtgc aaatgggcat ggaccatccc
     3361 gaactctaca gcgctcccga actctggctg tttgcctacg catttgcctt ccagatctat
     3421 tttgatttct ccggttatac cgatgttggc cgcggttccg cacttttatt cggc
//
LOCUS       DAOQNZ010000245         3466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13239_length_3466_cov_3.873351, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000245 DAOQNZ010000000
VERSION     DAOQNZ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13239_length_3466_cov_3.873351"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKW73_05650"
     CDS             complement(<1..186)
                     /locus_tag="PKW73_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72799.1"
                     /translation="MARDKAEPQKDVCSLDTVVSQGAKGQDLSDRFNKECPLETRKDV
                     FKQIMSAPDAGISTHGTD"
     gene            complement(249..1634)
                     /locus_tag="PKW73_05655"
     CDS             complement(249..1634)
                     /locus_tag="PKW73_05655"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNA72800.1"
                     /translation="MLKAKLPFYFAVACLLLGALLLPISNFLPRKIQNDRVGDGDFAS
                     FTKVMQESCADCHSSSSLAADPIYAHFPLASTLIARDRKEAQTVLNLSQAQLTGTEPI
                     SPPDLYRIASSVSNGSMPPGRYLALHWKGVSGLTEGDKRAVLRYVQAVSTRTQCRPLP
                     VSPASEPSLRGQLALREELGRKLFFEKRLSAHEKLSCAGCHAPDKGGADGLPIAIGAD
                     PSSGALNTPTVFNAALNFRQYWDGRASDLKQQALVALSSAGEMGGLAEDRLAELRSDS
                     AYSGLVERAYGVPLSPDVIADAIAVFEQTLITPGCKFDRYLKGEKTALSGQALKGYFL
                     FQEKGCVSCHSGMNLGGMTFEKMGIRGDYFAHRLKNGGKKVNEADLGRFNVTRLEADR
                     YRFKVPALRNVALTAPYFHDGSVSSLEEAVQDMARFQQGQELSREEVTCLVEFLKSLT
                     GKLNSQEQPEP"
     gene            1803..2420
                     /gene="folK"
                     /locus_tag="PKW73_05660"
     CDS             1803..2420
                     /gene="folK"
                     /locus_tag="PKW73_05660"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNA72801.1"
                     /translation="MSDPKSGSENSKGDALPEPGKKTGERKLRGNKRRVRSYIGLGSN
                     EGDRLGYVQQALQLLKDVKGIKVLETSSLYEAEPIGDVYTNWFVNAVVCIDTELSPFE
                     LLDVLKDIENRLYALHNKESGKVKERVIDLDILFYGQEVLDSVDLKIPHPRLLQRAFA
                     LVPLLEIAPDIRYPGLLKSVAEIHEELPEPEQVYLFGTRECDERD"
     gene            2430..2831
                     /gene="acpS"
                     /locus_tag="PKW73_05665"
     CDS             2430..2831
                     /gene="acpS"
                     /locus_tag="PKW73_05665"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNA72802.1"
                     /translation="MHFKIGSDICSVTRVEETLAKFGERFLDRVLTENEKAYVLSVAV
                     KDRASKVAARFAAKEATAKTLQTGFKGLSFKEIEVVRNSAGAPSLKLSGRASALAAKL
                     NLSSFELTISHEREYAIAFVLGYGETPNRSR"
     gene            2835..>3466
                     /locus_tag="PKW73_05670"
     CDS             2835..>3466
                     /locus_tag="PKW73_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNA72803.1"
                     /translation="MKTYGPVITFTSDFGTNDGYTGAVKGVILSRCLDARIVDICHQI
                     EPWNVASAAWIIASAYKYFPQGTIHIVVVDPQVGSSQRRILVVGDSELFLAPDNGILT
                     LVMKEQANWRAYELNKKEYWLPYVSNTFHTRDIFAPVAAHIASGIKPSEVGTEIPIES
                     LARLENQEVSTVGGVINGRVVHIDRFGNLITNISNDRVKPKANCYLDDLKV"
BASE COUNT          894 a          920 c          891 g          761 t
ORIGIN      
        1 atcagtgccg tgggtagaga tgccggcatc aggggcggac attatctgct tgaacacgtc
       61 ttttcgggtc tcaagcgggc attccttgtt gaagcgatcg ctcaggtcct gccctttagc
      121 accctgactt acgacagtat cgagagagca aacatctttc tggggttccg ccttatctct
      181 agccatgagc ttatgcctca agggaacatt ctattattcc cggcagaggc aactttcaag
      241 caaagacctc agggctccgg ctgttcctgg ctattcaact ttccagtcag gctcttcaaa
      301 aattccacca ggcaggtcac ttcctcccgg ctcagctctt gcccttgctg aaatctggcc
      361 atatcttgca cggcctcttc cagactggat acggaaccgt catggaaata aggcgccgtc
      421 agggcgacgt tgcgcagagc aggcactttg aacctatacc gatctgcttc cagccgggtg
      481 acgttgaaac gtccgagatc cgcttcattt accttcttac cgccgttttt cagcctgtgg
      541 gcgaagtagt cgcccctgat gcccattttc tcaaaagtca ttccacccaa attcataccg
      601 gaatggcagg aaacgcagcc cttctcctga aacaaaaaat agccctttaa cgcctgaccg
      661 gaaagcgcag tcttctcacc tttcagataa cgatcaaact tacaaccagg agtaatgaga
      721 gtctgttcaa agacggctat ggcatcggca attacatccg gggaaagagg aacaccgtag
      781 gctctctcaa ccagaccgga ataagcactg tcggaccgca gttcggcaag ccggtcttcc
      841 gccaggccgc ccatctcacc tgcgctagag agagcgacaa gggcctgctg tttcaagtct
      901 gaggcccgac catcccagta ctgcctgaag tttaaggcag cattgaagac cgtaggtgta
      961 ttgagcgctc cacttgaggg atccgcacca atagcgatgg gcaaaccatc ggcgccgccc
     1021 ttgtcaggag catgacagcc ggcgcaggag agtttctcgt gggcggatag gcgcttttcg
     1081 aagaagagtt ttctgccaag ttcttcccgg agagcaagtt gtccccgcag ggaaggttct
     1141 gaggctggag agaccggaag gggacgacat tgagttcgcg tcgaaaccgc ctgaacatac
     1201 ctcaatacgg ctctcttatc tccctcggta agccctgaaa cccccttcca gtgcaaggcg
     1261 aggtagcgtc cgggcggcat actgccattg ctcacggagg aagcgatccg gtagaggtcc
     1321 ggcggcgata tgggttccgt tcccgtcagc tgcgcctgag agagattgag cacggtctgg
     1381 gcctcctttc tatctcgagc gataagtgtc gaggccagtg gaaagtgcgc gtaaatcgga
     1441 tcggcggcaa gactactgct gctatgacag tccgcgcagc tttcctgcat tactttcgtg
     1501 aaagaggcaa agtccccgtc cccgactcgg tcgttttgaa tcttgcgagg caagaagttg
     1561 gaaatcggca aaagaagcgc gcccaggagc aggcaagcga ctgcaaaata aaaaggaagc
     1621 ttcgctttca acaagcacgt gcctcaaaga gtgccaagcc agtacctgtc gaatccggcc
     1681 agcgcttata ctaactctaa gtcttgattg actccaagaa ttgagagcag acactgctaa
     1741 aataacgctt actttcactt tcgaagccct gtttacagcc caccatgcac aaggaaagtc
     1801 aattgtccga cccaaaatca ggttctgaaa attccaaagg ggatgcgctc ccggagcctg
     1861 gcaaaaaaac cggggagcgc aagctcagag gtaacaaaag acgtgtaagg tcttatatcg
     1921 gcttaggttc caatgaaggt gacaggctgg gctatgtgca acaggccctg caattgctca
     1981 aagacgtgaa aggcatcaaa gtactggaaa ccagcagtct atatgaagcc gaaccgattg
     2041 gtgatgtcta caccaactgg tttgtcaacg ccgtggtttg cattgatacc gaactgagcc
     2101 cctttgaact tctggacgtg ctcaaggata tagagaaccg cctctacgcc ctgcacaata
     2161 aagaaagcgg taaagtgaaa gagcgcgtaa tcgacctgga catcctcttc tacggacagg
     2221 aagttctcga tagcgtcgat ctgaagatcc cgcaccccag gcttctacaa cgagcctttg
     2281 ccctggtgcc gctgttagaa atcgcaccgg atattcgcta tccgggtctt ttgaagtcgg
     2341 tagcagaaat tcatgaggaa ttgcccgagc cggagcaggt ctatctcttc ggtaccagag
     2401 aatgtgacga gagagactga tagcaaatta tgcattttaa aattggctcc gatatttgtt
     2461 cggtgacaag agtggaagaa accctggcta aattcgggga acgtttcctg gaccgagtct
     2521 tgaccgaaaa tgagaaagcc tatgttctct ctgtagcagt aaaagatagg gccagcaaag
     2581 tagctgcccg ttttgccgcc aaggaagcaa cagccaagac tttgcaaaca ggctttaaag
     2641 ggttatcgtt caaggaaata gaagttgtca gaaacagtgc cggtgcccca tcgcttaaat
     2701 taagcgggag ggcctcagcc ctggctgcaa aactcaattt aagcagtttc gagctgacca
     2761 tttcacatga acgggaatat gcgattgcct tcgtgctagg ctatggcgaa acaccaaaca
     2821 gaagcaggtg agctgtgaaa acttacggac cggtcattac tttcacatcg gatttcggaa
     2881 ccaatgatgg ttacacagga gcagtcaagg gagtaattct ttcacgctgc ctggacgccc
     2941 gtatagtcga catctgccat caaatcgaac cctggaacgt ggcttccgca gcctggatca
     3001 ttgccagcgc ctacaagtac ttcccgcaag gcaccatcca tattgtggtc gtcgatccgc
     3061 aggtgggctc cagccagaga cgaatcctgg tagtaggcga cagcgagcta ttcctggccc
     3121 cggacaacgg catcctcacc ctggtcatga aggaacaggc gaactggcgt gcctacgagc
     3181 taaataagaa agagtactgg ctgccttatg tctccaatac ctttcacaca agagacattt
     3241 tcgctccggt agccgcgcat atagcaagcg gcatcaaacc ttctgaagtg ggcacggaaa
     3301 ttcctatcga atctctggcg cgcctggaga atcaggaagt cagcaccgta ggcggtgtca
     3361 tcaacggccg ggtcgtgcac atcgatcgat ttggcaactt gattaccaat atctccaacg
     3421 accgagtcaa acccaaggcc aattgttatc tcgacgactt gaaggt
//
LOCUS       DAOQNZ010000246         3466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13240_length_3466_cov_3.196130, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000246 DAOQNZ010000000
VERSION     DAOQNZ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13240_length_3466_cov_3.196130"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKW73_05675"
     CDS             complement(<1..539)
                     /locus_tag="PKW73_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72804.1"
                     /translation="MRPAALTLLGLQFVGLMLAMVASCQAAFAQGPVRANGTGTNPGI
                     PQNGTESTAWANRQRYFDRNPWTKGSHDDDRTEDVPGTKNQKAVDAIPAFPMPVTFNV
                     ATPVDQSGQRWYMQMQSGVLPTKIQQRDIGNITRNYTTPGVFITDQSFAIHANQALSK
                     FAENQMDPERLNWNVATAAG"
     gene            complement(728..2773)
                     /locus_tag="PKW73_05680"
     CDS             complement(728..2773)
                     /locus_tag="PKW73_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72805.1"
                     /translation="MVNVLIALIVLIASTTILSGCSGVGGGLGRSDNAAMVPSGGGAL
                     GKLRPKVDPRYVEMAQQEVLADQEQQALRAQQAQSAQAQSGGGSFGGFSFDGLGRLLP
                     KVSREPISGAQPESAVAGSMNPLGQMGNSNGLPTGVTSAGSEAQAGGNNNTFGAAQEV
                     KPTELATYGNSYPGANPYGSSGTGVVPPPPPGSLVGGGALSPPPPAVTLSTRADVAYN
                     GADPNAAPNPYANPYANPYANPYANPYANPYLNPYAVPYQGGYPPQMAPQPERPAGLF
                     GAGQGQPRRSSSQEDEPVERKKSSFVPIIPRGMDARSPYQQRDDLKVLWKGALAGGAI
                     KNVVSKDGKLTETLSRVDVGLPADATRGSFNVNPRQVDAIFKPVAMDKKAYATVRRMQ
                     SDLVQSYYRYLYAYHKFALAQQTLSARKQEVEVAGSDSEKQRAAADVAQSQNDADSAK
                     EDLKAAQMELASTAGTNAARSIIGKVSGITPSSESLAVAEPEPPAPAENKGVFGFGSL
                     FGFGGKPAAEQPKEQPREKAVKVAAAPVAKAGKDKKVEGNGKKGAKTAAAAQPEGDLK
                     PAPAPVAASAEPAPQSTSGGISMELKGVNVTARKSVLTVAIRNNSENDFSFDPEAFAV
                     SENNHKLAEAALRADFETTLVQPNQEVKGTITIFGRPWNDRLSVSLSDGSRNVQLRR"
     gene            complement(2819..3445)
                     /locus_tag="PKW73_05685"
     CDS             complement(2819..3445)
                     /locus_tag="PKW73_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72806.1"
                     /translation="MNPNFLKMKYLPVLGAALALVLSCGSEAKAQGSVNLKKGQLQQA
                     GWHKSPLQVQILDDGPVVKDYRTAPANDQGFNIPIAPVGNGGGRIPEGGLPFQTGSGP
                     VMPRMQQSSLPQAGFGTNIPARGMGPAMALPQAKMGGLGNQYANQQKAATNVAARPVG
                     LGKPASGMRAAAAPASYGSSYGGSGGGGSYSGGGSSSSTSVRAKLLGK"
BASE COUNT          722 a          998 c          947 g          799 t
ORIGIN      
        1 ccggcggcag tggctacgtt ccagttcaag cgctcagggt ccatctggtt ttcagcaaac
       61 ttactgagag cctggttggc gtgaatggcg aaagattggt cggtaatgaa gacaccagga
      121 gtcgtgtagt ttctggtgat gttaccgatg tctctctgct gaatcttggt gggcagtacg
      181 ccggactgca tctgcatgta ccaacgttgg ccggactgat caaccggtgt tgctacgttg
      241 aaggtcacag gcattgggaa ggccggaatg gcatcgacag ccttctggtt cttggtaccg
      301 ggaacgtctt cagttctatc gtcgtcgtga ctgcccttgg tccagggatt gcggtcgaag
      361 tatctctgac ggttagccca ggccgtgctc tccgtaccat tttgaggaat accggggtta
      421 gtaccggtac cattggctcg cacagggccc tgagcgaaag cagcctgaca tgaagcaacc
      481 atggccagca tcaagccgac gaattgtaag ccgagaagcg tcagagcagc gggtctcata
      541 gccagcttca aagaagagcc agcgaatctg cattcactct tgctttgcgt atgctttgac
      601 ttggttgaaa aggctatttt catcattctt acctgatcta aatcttgctc tgacttttct
      661 ggtatctaca tttactaagt gtttaaaaaa aatccgaatc cgttaactgt cacccttctt
      721 tcatctgtca tcgcctcagt tgtacgttac gacttccatc cgacaatgac accgacaatc
      781 tatcattcca ggggcgacca aaaatggtta ttgttccttt cacttcctga ttgggctgca
      841 ccagagttgt ttcaaaatct gccctcaagg cagcctctgc taatttatga ttgttctcgg
      901 agacggcaaa tgcctccggg tcaaagctga aatcattctc gctgttgttg cgaatggcca
      961 cggtcaatac cgacttgcgg gcagtaacat tgacgccctt gagttccatg gagataccac
     1021 cgctggtgct ctgcggtgcc ggttcggcac tggcggcaac aggagccgga gccggcttca
     1081 gatcgccttc aggttgagca gccgctgccg ttttggcgcc tttcttacca ttgccttcta
     1141 cctttttatc ttttccggct ttagcaaccg gcgctgcggc aacctttacc gccttttcgc
     1201 gaggctgttc cttcggctgc tcagcggcag gcttgccgcc gaaaccaaac aatgagccga
     1261 agccgaatac acctttgttc tcagccggcg ccggcggctc cggctcagcc acggccaggg
     1321 actcagaaga gggagtgatg ccggacactt tacctataat ggaacgagcg gcattggttc
     1381 ccgccgtgga ggcaagctcc atctgagcgg ctttgagatc ttccttggcg ctgtcggcat
     1441 cgttttgaga ctgggccaca tcagccgccg ctctctgttt ttcagagtcg gaaccggcca
     1501 cttccacttc ctgcttacga gcagagagag tctgttgagc cagggcgaac ttatgataag
     1561 cgtagaggta tctgtaatac gactgcacca gatcactctg catgcggcgc accgtagcat
     1621 acgctttctt gtccatggct accggtttaa agatggcatc gacctgacgc gggttgacgt
     1681 tgaagctacc ccgtgtggca tcggcaggca aaccgacatc gactctcgaa agagtctcgg
     1741 tcaacttacc atccttagag accacattct taatggcacc accggcaagg gcacccttcc
     1801 agagaacttt caaatcatcg cgttgttggt aaggtgatct ggcatccatg ccccggggaa
     1861 tgatcggtac gaagcttgat ttctttcgct ccaccggctc atcctcctgg gaggaactgc
     1921 gacgcggctg gccctgcccg gccccgaaaa gccccgccgg tctctccggc tgcggcgcca
     1981 tctgaggcgg atagccgccc tgataaggaa cggcataggg atttagataa ggattggcgt
     2041 atgggttagc gtaaggattc gcataagggt tagcataagg attggcatat ggatttggcg
     2101 cggcgttagg atcggcacca ttgtatgcca catcggcgcg agtgctcagg gtcactgccg
     2161 gcggtggcgg agacagggcg ccgcctccca caagcgaacc gggcgggggt ggaggcacca
     2221 caccggtccc actggaaccg taagggttgg caccaggata gctgtttccg taggtagcca
     2281 gttccgtggg cttcacttcc tgggcggcac cgaaagtatt gttattaccg cctgcttgcg
     2341 cttccgaacc ggcactggta acaccagtcg gcaagccgtt gctgttgccc atctgaccaa
     2401 gcgggttcat ggatccggcc acggcactct ccggctgggc accggaaata ggctctctgg
     2461 agaccttagg aaggagtcga ccaagaccat cgaagctgaa accgccgaag ctgccgccgc
     2521 cactctgggc ttgcgcactc tgggcctgct gggcgcgcaa ggcctgctgc tcctgatctg
     2581 ccagcacttc ttgctgagcc atctccacgt agcggggatc caccttcggt ctcaatttac
     2641 cgagagcgcc tccaccgctg ggaaccatgg cagcgttgtc gctacgcccc aatccaccac
     2701 caactccact acaaccgctg aggattgtag tggaggcaat gagtacgatt agagctatta
     2761 ggacgtttac catgtcttgg ttgaaagacc tcctgagtct ttgaaactac ctgcctcgtt
     2821 acttccctag caacttggct cgaaccgatg tggatgaact ggaaccgcca ccggagtagc
     2881 tgccgccgcc gccactgccg ccgtaactac tgccgtagct ggctggggct gccgcagccc
     2941 gcatacccga agcaggcttg cccagcccga caggacgggc agccacattg gtggcggctt
     3001 tctgctgatt ggcatattga ttaccaagcc cgcccatttt ggcttgcggc aaggccattg
     3061 ccggtcccat accgcgagcc gggatattgg ttccgaaacc ggcctggggc aggctgctct
     3121 gctgcatgcg cggcatcacc gggcccgaac cagtctggaa aggcagtccg ccttcgggaa
     3181 tacgaccgcc gccattgccg acgggagcaa tgggaatgtt gaaaccctga tcgttagcgg
     3241 gagcagtccg atagtcctta accaccggac catcatcgag aatctgcacc tgcaagggcg
     3301 acttgtgcca gccggcttgc tgcaactgac cctttttcaa attgacggac ccctgagctt
     3361 tcgcctcgga accgcacgag agaaccagcg ccagtgccgc tcccaacact ggtaaatact
     3421 tcatttttag aaaattcgga ttcataactc taacctctta aatccc
//
LOCUS       DAOQNZ010000247         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13273_length_3461_cov_3.368174, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000247 DAOQNZ010000000
VERSION     DAOQNZ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13273_length_3461_cov_3.368174"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="PKW73_05690"
     CDS             <1..1581
                     /locus_tag="PKW73_05690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72807.1"
                     /translation="WLASSMTLFTMLHDYEDTVKNQQFYAFLASCLRFLDTYPQMLWQ
                     TFNSSQLLFASAIGVVGIMVIWVLAVFFLFLALKIMISPNRLAINREGIELDFWTDIM
                     STETDRVKFSDVEKVELVGASDSANPEKLEILVTTKPWETPLGPLASVLTLKLSAIRK
                     ADREKFLKALEEFAPARATSTEVVEALKEGAENSYTELWLQSLNESAIRKNIQPLCEG
                     DLLQEGRYQIVRRLGIGGEGTAYEAFDRSNLAHGKVERIVLKETIMPPYSDRSQEQES
                     LSRIKKESEILSSLNSPLVVACHGFFLEDRRSYLVLEYIEGENLRELVRKQLVKQQGA
                     MEQEQVIELALIMCDILTVLHDNQIIHRDFTPDNLIYKAERTNEGSVGQLKLIDFAVA
                     TQDKGGTTGTIVGKHSYVPPEQFRGHAQYRSDIYSMAATIYYLLTGQDPEPITQANLD
                     HRKDLCPALIELIKAATSQNPEERPESARQFKEMLLNIKTQIADENQNSTSEVDQEPL
                     KIKIGEREKEELRWQNSA"
     gene            1563..2960
                     /locus_tag="PKW73_05695"
     CDS             1563..2960
                     /locus_tag="PKW73_05695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72808.1"
                     /translation="MAELCIKVHYQDPVRDGIVTTFKLLFPFWGILPFVFALMGLSMG
                     LVSLGAALTHVMLWLLIPLLSLIFLRLISSHQILMDKRGIEIPGDFTQGIVKSRYIPW
                     AEVSKVFVSGDNQKTVVLMTSKDNLAVKLPANRMDVAELEKLLVTIDLLLEKGKDESL
                     KELALTLKQNKDENLGLSYTDIWEDELKRRFRPASFMPLEKDVLVRNGSLKIVRPLAS
                     GGLSAVYLASTQSGSLVVLKESVIPEGFDSQLQEKAREMFAREAKILMQLSCDSIVKV
                     LDYFTEGGRSYLMLESVTGPDLRQYVKQHGKVRESLVLDLGLQMAEILCFLHAQNPPV
                     IHRDFTPDNLVLQNDGKLIAIDFGAANEFIGTATGTFVGKHAYIAPEQFRGKATPQSD
                     LYAMGGTLFYLLTGEEPEALSASDPAVKVPVSSQWSSLILDMTNPDAAARVTTAEVVK
                     ARLTLIKEGDAAKMH"
     gene            complement(3044..>3461)
                     /locus_tag="PKW73_05700"
     CDS             complement(3044..>3461)
                     /locus_tag="PKW73_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72809.1"
                     /translation="PVTGFVTLERRLAGPLSLMGRVAASFGSTTASADSVVVDATSTG
                     ASVMGALGLKVSFGSRDTLEVSPFALLDGAYGWAGGAAAHAFVSSLVVAVKEATGKGS
                     FCAFLSCYMKLFIGQLLTPFIVAFLNFAQDKLPLKA"
BASE COUNT          875 a          903 c          921 g          762 t
ORIGIN      
        1 tggctggcta gcagcatgac gctttttacc atgttgcacg actacgagga taccgtcaaa
       61 aaccaacagt tttatgcgtt tctcgcatcc tgcctgcgtt tcctggatac ttatccgcag
      121 atgctctggc agactttcaa tagttcccag cttctctttg cctctgcaat tggagttgtg
      181 ggcattatgg tgatctgggt gctggcagtc tttttcctct tcctggccct caagattatg
      241 atcagcccca atcgactcgc gattaacagg gaaggcattg agctggattt ctggacggac
      301 attatgtcca ctgaaacaga cagggttaaa ttcagtgatg tggagaaggt agaactcgtg
      361 ggggcaagtg attcggccaa cccggaaaag ctggagattc ttgtgaccac aaaaccctgg
      421 gagactcccc tgggacctct ggcgagcgtc ctgaccttga aactgtctgc tattagaaaa
      481 gccgaccggg aaaaatttct taaggctctg gaggaatttg ccccggccag ggctacctca
      541 accgaggtgg tggaagccct gaaggaagga gcagagaatt cttacaccga gctctggttg
      601 cagagcctga acgaatctgc cataagaaag aacatccagc ctctgtgtga gggagatttg
      661 cttcaagagg gtcgttatca aattgtcaga cgtctgggca taggcggtga aggcaccgct
      721 tacgaagctt ttgatcgaag caatctggcg cacgggaagg tggaaaggat tgtcttgaaa
      781 gagaccatca tgccgcccta ttcagaccgc tcccaggagc aggagtccct cagtcgcatt
      841 aagaaagagt cggagatact cagtagcttg aacagccctc tggtagtggc ttgccatggc
      901 ttcttcctgg aagaccggcg ctcttatctg gtgctcgagt atattgaagg cgaaaacctg
      961 cgcgaattag tgaggaagca actagttaag cagcagggtg cgatggagca agagcaggtc
     1021 atagagctgg ctctcatcat gtgcgacatt ttgactgttt tgcacgacaa ccagattatt
     1081 caccgggatt tcacacccga taatctcatc tacaaagctg agagaactaa tgaaggttcg
     1141 gtaggtcaac tcaagttgat cgacttcgcc gtggccacgc aagacaaggg cggcaccacc
     1201 ggtactatcg tgggcaaaca ctcttatgtg ccgccggagc aattccgcgg ccacgcgcag
     1261 tatcgaagcg acatttattc catggccgcc accatttatt atctgcttac cggtcaagac
     1321 ccggaaccca tcacccaggc aaacctggac cacaggaaag acctctgtcc ggcattaatt
     1381 gagctaataa aagcagccac cagtcaaaat ccggaagaga ggccggagag cgcccggcag
     1441 ttcaaggaaa tgcttttgaa cattaaaacg cagattgcag acgaaaacca aaactccact
     1501 tcggaagtag accaggagcc cctcaaaata aagatcggcg aaagagagaa agaagaattg
     1561 cgatggcaga actctgcata aaagtccact accaagatcc tgtcagagac ggtatcgtca
     1621 cgacctttaa actgctcttt cccttctggg gtatcttgcc ctttgtcttc gccctcatgg
     1681 gactttccat gggcttagtg tcactgggcg cggctcttac tcatgtgatg ctctggttac
     1741 tgatcccgct tctttccttg atttttttgc gcctcatctc gtctcaccag attctcatgg
     1801 ataaacgcgg cattgaaatt cccggcgact tcacccaggg tattgtcaaa agccgctaca
     1861 taccctgggc ggaagtttcc aaagtttttg tcagcggcga caaccagaag accgtggtgc
     1921 tcatgaccag caaggacaat ctggcggtga agttgccggc aaacagaatg gacgtggcag
     1981 agttggaaaa gctactggtc accattgacc ttcttctgga aaagggaaaa gacgagagct
     2041 tgaaagaatt ggccctgaca ttgaaacaga acaaggatga aaatctcggt ctcagctaca
     2101 cggacatctg ggaagacgag ctaaaaagac gctttcgccc ggcttctttt atgcccctgg
     2161 aaaaagatgt acttgtgcgc aacggcagct tgaaaattgt cagacccctg gcctcgggcg
     2221 ggctgtcggc agtctatctg gcttccaccc agagcggcag cctggtggtt ttgaaagaat
     2281 cggtgatacc ggaaggcttc gattcgcagt tgcaagagaa agccagagag atgtttgccc
     2341 gcgaagcaaa gatactgatg cagttgtctt gcgattcaat tgtgaaagta ctggattact
     2401 ttaccgaagg cggccgttct tacctgatgc tggagagcgt gaccggacca gatttgaggc
     2461 aatacgtcaa gcaacatgga aaggtgcgcg agtctctggt gctcgacctg ggtctgcaga
     2521 tggcggaaat tctctgtttc ttacacgccc aaaacccgcc tgttattcac cgcgacttca
     2581 ctcccgacaa cctggttctg caaaatgacg gcaagctcat tgccatcgac tttggtgccg
     2641 ccaatgaatt tatcggcacc gccaccggta cctttgtggg caagcatgct tatatcgccc
     2701 cggaacaatt tcggggcaaa gccacgcccc agagcgattt atacgccatg ggcggcacac
     2761 tcttttacct tctcaccggc gaagaaccgg aagcactcag tgcctcggac cctgccgtga
     2821 aagtgccggt gagcagccag tggagcagcc tcatactgga catgaccaat cctgatgccg
     2881 ctgcccgcgt taccactgcc gaggttgtga aggctagatt gaccttaatc aaggaagggg
     2941 acgctgccaa gatgcactaa ggtgaatccg ctgccccgtg gtaaggctca ctggctgcca
     3001 tttttacggt tatggtcccc aagagcaagg caaacctcta taattatgct ttcagaggta
     3061 acttgtcttg agcaaaattc aaaaaagcga cgatgaatgg cgtaagcaac tgtccgatga
     3121 agagtttcat gtagcaagaa agaaaggcac agaaagagcc tttaccggtc gcttctttga
     3181 ctgccacgac aagggaactt acaaatgcgt gtgctgcggc gccgcccgcc catccatagg
     3241 ccccgtccaa cagcgcgaac ggcgagacct cgagcgtgtc cctcgacccg aacgagacct
     3301 tgaggccgag cgcgcccatc acggaggctc cggtcgacgt ggcgtcgacc acgactgaat
     3361 ccgcggacgc ggtcgtgctc ccgaagctcg ccgcgacgcg acccatgagg gagagcggac
     3421 ccgcgagcct gcgctcgagc gtgacgaatc cggtcacggg g
//
LOCUS       DAOQNZ010000248         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13281_length_3460_cov_4.611454, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000248 DAOQNZ010000000
VERSION     DAOQNZ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13281_length_3460_cov_4.611454"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKW73_05705"
     CDS             complement(<1..440)
                     /locus_tag="PKW73_05705"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA72810.1"
                     /translation="MIFPILKRLFMAVPVLLIVASLTFLLVRIVPGGPFDSEKSLPPE
                     IVANLNAKYHLDKPVHEQYVLYIGRLCRGDLGVSYKYVNRTVNDILFDAFPVSCQLGF
                     VALVLAVAFGVPLGAIAALGRGGRQDFLAMFVSTLGVSVPGFVIG"
     gene            complement(437..1978)
                     /locus_tag="PKW73_05710"
     CDS             complement(437..1978)
                     /locus_tag="PKW73_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNA72811.1"
                     /translation="MRKPYEDGVLRINLGTEPPGLDWHTSTDSTSFDVVSNLMVGLTQ
                     YTNELEAKPACAASWEVLDGGKRYLFHLRRDVFWTDGKPVTAYDFEFAWRRLLAPETA
                     AQYAFFLYDIVGARAFNTGASKDVNTVGLKALDPYTFEVKLAKPAAYFIYLTAFCPSF
                     PQRKDIVERYKDRWTEPENLVTNGPFKLSYWQHEYKIELTANEKFFEGAPKLKRIKMF
                     MVPEASTAFALYENNELDFVDNRSFSTPDVERFRHSPQYKNFPLLRAYYLAFNVSKKP
                     FDNADVRRAFALSIDRKVFPKILRRGERPTSSWIPPALGGGGSDSGLDFNPEEGRRLL
                     ARAGYPGGKGFPETKLLYPNREDVRLVVEATQDEIKRNLGVKVELVNQEWKVYLATTR
                     KDPPQMFRDSWGADFPDPETFMNLFTSYNGNNHTRWHSAEYDRLIEAAEGEQDKNKRA
                     ELYRRADVMLCREAVPIVPAYLATQNIMVKPWVKGIAINPLDFQFFKTVTVGDTAAGE
                     VSKSP"
     gene            2401..2568
                     /gene="rpmG"
                     /locus_tag="PKW73_05715"
     CDS             2401..2568
                     /gene="rpmG"
                     /locus_tag="PKW73_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNA72812.1"
                     /translation="MAKKDDRIIITLACGECKRRNYTTMKNKRNDPERLNLNKYCKFC
                     RRTVEHKETKK"
     gene            2612..2682
                     /locus_tag="PKW73_05720"
     tRNA            2612..2682
                     /locus_tag="PKW73_05720"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2644..2646,aa:Trp,seq:cca)
     gene            2709..3068
                     /gene="secE"
                     /locus_tag="PKW73_05725"
     CDS             2709..3068
                     /gene="secE"
                     /locus_tag="PKW73_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNA72813.1"
                     /translation="MATTNEDDGEQVGTQTKAGGVKMVESKSEEQDKQPKKNPTEKEP
                     SGFAGAAQFLREVAIEHRKITWPDRRQIVRETWSVIFLVSAITVMVLGIDWVLGHVIF
                     GPLEHWARIHGAGVGRG"
     gene            3085..>3460
                     /locus_tag="PKW73_05730"
     CDS             3085..>3460
                     /locus_tag="PKW73_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HNA72814.1"
                     /translation="MGFIKTDGQRRWYAVQTASGHENKVKEHIEKRILTMGAQDRIFG
                     VIVPEKMVTKVKDGKRVEKREREYPGYVFVEMILDDDSWRVVREAPGVTKYVGAGKKP
                     TPVQDSEIRKILRRQMAMTGMKG"
BASE COUNT          898 a          881 c          956 g          725 t
ORIGIN      
        1 cctattacaa acccgggtac ggacactccc agggtggaga cgaacatggc tagaaaatcc
       61 tgccgcccgc cccgaccaag agcggctatt gcacccaggg gcacaccgaa agccacagcc
      121 agaactagcg cgacaaaacc gagctgacag gaaacgggaa aggcatcaaa gagaatgtcg
      181 ttgacggtgc ggttgacata cttgtacgag acccccagat cgccgcggca gagacgcccg
      241 atatagagca catactgctc atgcaccggc ttgtccaggt gatatttggc attcaggttg
      301 gcaactattt cagggggcaa actcttttcc gaatcgaagg gaccaccggg cacgattcgc
      361 accagaagga aagtaaggga agcgacaatc aagagcaccg gcacggccat gaagagtctt
      421 ttgagaatgg gaaagatcat ggactctttg agacctcccc ggccgcggta tcacccacgg
      481 tgacagtctt gaagaactgg aaatcgagag gattgatggc tatgcctttt acccagggct
      541 tgaccattat gttctgcgta gccagatagg ccggcacaat aggcaccgct tcccggcaga
      601 gcattacatc cgcccgtctg tacagttcag cccgcttgtt cttatcctgc tccccttccg
      661 ccgcttcgat gagccggtcg tactcggcgc tgtgccagcg tgtgtgattg ttgccgttat
      721 agctggtgaa gagattcata aaggtctcag ggtcagggaa atcggcaccc caggagtctc
      781 tgaacatctg tgggggatcc ttgcgggtag tagcaagata aaccttccat tcctgattga
      841 ccagttccac cttaacgccc agattgcgct tgatctcatc ctgagtggct tccaccacca
      901 ggcgcacatc ttcgcgattg ggataaagaa gtttggtctc aggaaaaccc ttgccgcctg
      961 gatagccggc ccgggccagt aagcggcgac cttcctcggg attgaaatcc agaccggagt
     1021 cactgccgcc gccgcccaga gccggcggaa tccagctact ggtcggtctc tcgccccgac
     1081 gcaagatctt gggaaagacc ttgcggtcta tactgagggc aaaggcgcgc ctgacatcgg
     1141 cattgtcgaa aggcttttta ctgacgttga aagccagata gtaggcccgc aggagcggga
     1201 aatttttata ctgcggggaa tggcgaaagc gctccacatc aggggtcgag aagctccgat
     1261 tgtccacgaa atcgagttcg ttattctcgt aaagagcaaa ggcggtggaa gcctccggca
     1321 ccatgaacat cttgatgcgc ttgagtttgg gagccccttc gaagaatttt tcattggccg
     1381 tcaactcaat cttatattcg tgctgccagt aagaaagctt gaaaggaccg ttggtaacca
     1441 ggttctccgg ctcggtccaa cgatctttat agcgctccac aatatccttg cgctggggaa
     1501 aactgggaca gaatgccgtc agataaatga agtaggcggc aggcttggcc agcttcactt
     1561 caaaggtata ggggtccagg gctttcagac cgaccgtgtt cacatccttg ctcgcaccgg
     1621 tgttaaaagc ccgggcaccg acaatgtcgt aaaggaagaa ggcgtattgg gccgccgttt
     1681 ccggagccag gagacgacgc caggcaaatt cgaaatcgta ggcggtgaca ggcttaccgt
     1741 ctgtccaaaa gacatcgcgg cgcaggtgaa agagatagcg cttgccgccg tccagaactt
     1801 cccaactggc tgcgcaggct ggttttgcct ccaactcatt ggtgtactgg gtgagcccga
     1861 ccatcaaatt ggaaaccaca tcgaaagaag tggagtcggt ggaagtatgc cagtcgagac
     1921 ccggaggctc ggtgcccagg tttatgcgca gaaccccgtc ttcatagggt tttcgcaaat
     1981 gggacagctc ttcggcgcag cccccctgaa taaaacaagt acatataaat agaacgaaga
     2041 gggtcaatga tgagagccct ctagtaatga cggaaccgtc tccaagaggt agttttcgcc
     2101 ccctggcaac aagagttgcg agacgattct taacgaagca aattaaatgc ctgccatctt
     2161 cagcccctga attgagtttt ttcgccctca tgagcctcaa agtagaaaat ctcgctgaca
     2221 aagttaccgc ttgctaattt tggtaagggt tggccctgaa aatagatatt ctagctttgt
     2281 tgacagtttt tcgggctctt ggatatactg acttgacgcc tccgaggagg cctttttggt
     2341 ttgcgttctt tttttgagaa cgctttatac cgtagtgaac tagtccgtgg gagtttagtc
     2401 atggctaaga aagatgatcg gatcatcatc accctggctt gtggagagtg caaaagacgc
     2461 aactacacaa ccatgaaaaa taagagaaac gatcccgaaa gactcaacct caacaagtac
     2521 tgcaaattct gccgtcgcac tgttgagcat aaagagacca agaaataaac accttacaaa
     2581 ataaggtagg acccgcttgt cgggtctcag tgcgccctta gtttaacggt agaacggggg
     2641 tctccaaaac ctttgatgag ggttcgattc cttcagggcg cgaattatat agtggataag
     2701 ctaacaagat ggcaacgaca aacgaagatg acggcgaaca agtcggcacc caaaccaaag
     2761 ctggtggcgt gaagatggtt gagtcgaagt ccgaagagca agacaagcaa cccaagaaaa
     2821 atccgacgga aaaagagccg tccggttttg caggggccgc ccagttcttg cgcgaagtag
     2881 ccatcgagca ccgcaaaatc acctggcctg accgccgtca aattgtgcgc gaaacctgga
     2941 gcgtaatctt cctggtctcc gcgatcacag taatggtcct ggggattgac tgggttctgg
     3001 gacacgtgat cttcggacca cttgagcact gggctcgcat ccacggcgcc ggcgtgggca
     3061 ggggttaagg agcaaattca aacaatgggc tttatcaaga ctgacggtca gagacgatgg
     3121 tatgcggtgc aaaccgcttc cggtcacgaa aacaaagtga aggaacacat cgagaagcgc
     3181 atcctgacaa tgggtgccca ggaccgtatc ttcggcgtga ttgtgcctga gaagatggtc
     3241 acaaaggtga aggacggcaa gcgcgttgag aagcgcgagc gtgagtaccc cggctacgtc
     3301 ttcgtggaaa tgatccttga tgatgactcc tggagagtgg tgcgggaagc ccctggtgta
     3361 acgaaatatg ttggcgccgg caagaaaccg acaccggtac aagactccga aattcgcaag
     3421 atactgcggc gccagatggc catgaccggc atgaagggca
//
LOCUS       DAOQNZ010000249         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13283_length_3460_cov_4.180617, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000249 DAOQNZ010000000
VERSION     DAOQNZ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13283_length_3460_cov_4.180617"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKW73_05735"
     CDS             complement(<1..560)
                     /locus_tag="PKW73_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72815.1"
                     /translation="MFVWSERKSQLDDKERLEQIIGSADGLNQRAMGLFPHPREEQPR
                     RVDFASQIKCRQLLVNIEGQEETCVLRLISQRNKSRAAVLILRGRVLGCVYGRKEHDG
                     ELLQLMGEEAFQRVGLEILSSDTIIDTYKIDDKTAIAASAIFHGEPYQAPSTQPVKER
                     LDFALKHLMDSLMPGTIILNEGIDNAA"
     gene            complement(689..1189)
                     /locus_tag="PKW73_05740"
     CDS             complement(689..1189)
                     /locus_tag="PKW73_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNA72816.1"
                     /translation="MKETAIAKNRRQSLGKLGEELAAQHLSGLGLEILARNFHHGREG
                     EIDIIALEPESDSASEKDCLVFIEVKTRRIDSPTSAFEHTGQMSVDRRKRQKIIRAAF
                     AYLSSARERLSTLSSWGSGRMPMRFDLILIDIALTRSQLKAYVDAMDQPALAKNSRIQ
                     HFKELF"
     gene            1261..2544
                     /gene="serS"
                     /locus_tag="PKW73_05745"
     CDS             1261..2544
                     /gene="serS"
                     /locus_tag="PKW73_05745"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNA72817.1"
                     /translation="MLDLKLIREKADFVEAQLARRHGGFSIKPIVELDGRYRKAQAEW
                     ENLNRRRNEISESFKTGKLPKEELEALRKEASSAKEKQDQVNEERVNLEQELNQMLLS
                     IPNLPAEEVPDGEDENGNKEISTWGTIPSIKNPRHHYEIGTDLGIFDFERGVKIAESR
                     FTVLMNWGAKIERALMNLMLDTHAGKGYVEVFPPILVNRDCLVGTGQLPKFEGDYYKL
                     ADDDLYLVPTAEVPVTNLYREEVLEEEKLPILHCAYTPNFRREAGSAGKDTRGYIRQH
                     QFNKIELVKFTTPEASEEEHEKLTRDAETILEMLQLPYRRVLLCSGDIGFCARKCYDL
                     EVWFPAQDKYREISSCSNFGDFQARRAGLKYRPKDGGKPRFLHTINGSGLAIGRTLAA
                     ILENYQNEDGSVTVPEVLQSYLGGIKRIEPQLTHA"
     gene            2537..3331
                     /locus_tag="PKW73_05750"
     CDS             2537..3331
                     /locus_tag="PKW73_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72818.1"
                     /translation="MPEFSQASALSSAPSPMRRHRFVQAACLFSAVAHVAATLICVYT
                     FKEGNFLQFSPVKLMQFVPEHIWLWRSSCLLVMLSSVSTLVFILAMREVLEEKYRFLV
                     GIAVCFSLIACSQDLAAIARMMVLFSDVCLQGVLSGSLANVRADYAQSGWFILNESIT
                     QSFLLASFLYGMGGLCIALCLTRTKILESRLAFAHLPVWLVMIASSVTTFFGLLPASV
                     VLLFLANLGISLISAFSGVATDAVLKSPLARAPGEAAVDLDNSGFF"
BASE COUNT          729 a          911 c          930 g          890 t
ORIGIN      
        1 gcggcattgt ctatgccttc attgaggatg atggtgccgg gcatgaggct gtccatcaag
       61 tgcttgaggg caaaatcgag gcgttctttg acgggctggg tgctgggggc ttgatagggc
      121 tcgccgtgga agatagccga agcggcaatg gctgttttgt cgtcaatttt gtaagtgtcg
      181 atgatggtgt cggaggagag aatttccaga ccgaccctct gaaaagcttc ttcgcccatg
      241 agctgcagca gctcgccgtc gtgttctttg cgcccgtaga cgcaaccaag aacgcgaccc
      301 ctcaaaatca ataccgcggc ccgggactta ttgcgctggg agatgagcct cagaacgcag
      361 gtctcttcct gtccttcgat gttgaccagt aattgtctgc atttgatctg gctggcgaag
      421 tctacgcgcc ggggctgctc ttccctcgga tgggggaaaa gacccatggc cctctggttg
      481 agaccgtcgg cactgccaat tatttgttcc aggcgctctt tatcgtcgag ctgacttttt
      541 ctttcagacc agacaaacat atccttcctc tccctgccct gttgatattt tatccaggcc
      601 cgggctgttt ccacttacaa ccgccgggag aaactgcttc aacttgggtt aaagatattt
      661 caatcggcgc caggcagaga cgacgagctt agaaaagctc tttgaaatgt tgtatgcggg
      721 agtttttagc cagggccggc tgatccatgg cgtcgacgta ggcttttagc tgtgaccttg
      781 tcagggcaat gtcgataaga atcagatcga agcgcatggg catgcggccg ctgccccaac
      841 tgctcagggt ggaaagccgc tctcttgccg aactcagata ggcaaaggcc gccctgataa
      901 ttttctgcct cttgcgcctg tctacggaca tctgccctgt atgttcgaaa gcactggtgg
      961 gagaatcgat gcgcctggtt ttcacctcga tgaaaaccag acaatctttt tcagaggcag
     1021 agtcagattc aggttcgagg gcaattatgt ctatttcacc ctctctgccg tggtggaaat
     1081 ttctggctag aatctcaagg ccaaggcctg acaggtgctg ggctgccagt tcttccccca
     1141 gtttgcccag gctttgtcta cggttcttag caatagctgt ctctttcatg caggtgaccc
     1201 tcctgagtgg ctataatcga ctttccttta ataacgtccc gcagccctga gaagttcaaa
     1261 atgctcgacc tgaaacttat ccgtgaaaaa gccgattttg tagaagccca attggccaga
     1321 cgacatggcg gcttttccat caagcccata gtcgaactgg atggacgcta ccgcaaggcg
     1381 caagccgagt gggagaatct caaccgccgt cgcaatgaga tttctgagtc gtttaagacc
     1441 ggcaaattgc ccaaggaaga attggaagct ttgcgcaagg aagccagtag cgccaaggaa
     1501 aagcaggacc aggttaacga agagagagtc aatctggagc aggaactgaa tcagatgttg
     1561 ctttccattc ccaacctgcc ggcggaagaa gtgcctgacg gtgaggatga gaacggcaac
     1621 aaggaaattt ctacctgggg caccatcccc agtatcaaaa atcctcgcca tcattatgaa
     1681 atcggtaccg acctgggtat cttcgacttt gagcgcggag tcaaaattgc cgagtcgcgt
     1741 ttcacggtgc tgatgaactg gggcgccaaa atcgagcggg ctctcatgaa tctgatgctc
     1801 gatacccatg ccggcaaagg ctatgtggag gtctttccgc ctatcctggt gaaccgtgac
     1861 tgcctggttg gtaccgggca attgcccaag ttcgaaggcg attattacaa gcttgccgac
     1921 gatgatctct atctggttcc cacggcggaa gtgccggtca ccaacctcta tagagaagaa
     1981 gtgctggagg aagaaaagct tcccattttg cactgcgcct atacgccgaa cttcagaaga
     2041 gaagccggtt ctgccggaaa ggacaccagg ggctatattc gtcagcatca gttcaataag
     2101 attgaactgg ttaagttcac taccccggaa gcttccgaag aagagcatga aaagctgact
     2161 cgcgatgctg agaccattct ggaaatgttg cagcttccct atcgccgtgt cttgctttgt
     2221 tcgggcgaca ttggattctg tgctcgcaaa tgctatgacc tggaggtctg gttcccggcc
     2281 caggacaagt acagggaaat cagctcctgc tccaactttg gagacttcca ggctcgtcgt
     2341 gccggtctca agtaccgccc caaggatggt ggcaagcctc gtttccttca caccataaac
     2401 ggttccggtc tggccatcgg tcgcacttta gcggccatac tggagaacta tcaaaacgaa
     2461 gacggttcgg tgactgtgcc tgaggtactg cagtcttatc tgggcggcat aaagcgcatt
     2521 gagcctcagc ttacccatgc ctgagttcag ccaggcaagc gccctttcgt cggctccgtc
     2581 gccaatgcgc cgacatcgct tcgtgcaggc tgcttgtttg ttttctgctg tggcccatgt
     2641 ggctgccact ctcatttgtg tctacacatt caaggaaggc aacttcctgc aattctctcc
     2701 cgtcaagctc atgcaattcg tgcctgagca tatctggctc tggcgctctt cctgtcttct
     2761 cgtaatgctt tcctcggttt ccaccctggt tttcattctg gccatgaggg aagttctgga
     2821 agagaagtat cgctttctgg taggtatcgc cgtctgtttc tccctgattg cctgcagtca
     2881 ggatctggct gccatagcca gaatgatggt gcttttctcc gatgtttgct tgcagggagt
     2941 gctgagcggc tctcttgcta atgtgcgggc cgactacgcc cagagcggct ggttcattct
     3001 caacgagtcc attacccaga gttttttact ggcgtctttc ctttatggca tgggtgggct
     3061 ctgcatcgca ctttgcctga cccgcaccaa aattctggaa agtcgcctgg ccttcgctca
     3121 cttgccggtc tggctggtga tgattgccag ttcggtgaca accttcttcg gtcttttgcc
     3181 ggcctctgtg gtgcttctat ttctagccaa tctcggtatc tctttgatca gtgccttttc
     3241 cggcgtggct accgatgccg tattgaagtc gcctctggcc agggctcccg gagaggctgc
     3301 cgtggatctc gataattcag gtttcttttg atgcaagttt tggcgaatgc ggactctgca
     3361 tgggcgcccg cgcctagccg tagctctctt tgcggaatca gtgcaaatgt aacagcgatt
     3421 ccgcagagtg gctgttgaac cgggcttaga cgcagtggca
//
LOCUS       DAOQNZ010000250         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13342_length_3451_cov_3.175501, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000250 DAOQNZ010000000
VERSION     DAOQNZ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13342_length_3451_cov_3.175501"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PKW73_05755"
     CDS             complement(<1..342)
                     /locus_tag="PKW73_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA72819.1"
                     /translation="MKKVNVLIVEDNELMRQGLRSVLERTEEFCVVGEAEDGEEAVRL
                     AQTLKPDVIVMDIGLPYKNGIEATRDIKQNLPSVNIVMLTSHDNDDEIFAALSAGAQG
                     YCLKDCASERIK"
     gene            493..2586
                     /locus_tag="PKW73_05760"
     CDS             493..2586
                     /locus_tag="PKW73_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNA72820.1"
                     /translation="MSDLRKLTGRTHFRDTLTSLFHITKALIQLLLVFGTLGGTVIGL
                     LVVQELMRIPDLSFLKDYKPVDAISIYDKNDELIETIDAGIPRTVIAYKDIPRVLILA
                     VMAAEDKHFFEHHGVSPQGILRATLVNARSMQMKEGGSTITQQLAKNLFFLDGKRTGI
                     VKLAEMFVAGRLEEKFSKEEILSLYLTEIYFGEGARGIEQAAQTYFGKSARNLNLEEA
                     AYLAGIIRSPSYLGSAKHRDAALERQRQVLRSMQESNYITMEAEMRAEATPLNFRKTA
                     LKRQKTYPFTRFPYFTSYVLELVRNSYSDMTIGMNGMKIYTTLDPVAQSLAERTLVSE
                     IRRAPVGIEEEALVALSVKDNAIRALVGGAHNYWDNQWNCAVNPHTAGSSIKPFVYLT
                     AFMAGVATPESTVLDAPFELKEANGEKWTPKNFDGKYLGNITIRDALMQSRNMCTIRV
                     TEQVGINSIISTMNSAGIRTKMAPTLALALGSSAVTPLELASAYGTLARGGLATPAWT
                     IRRIEDARGKILSYYQPSICRVFPQEQVTWLTDILQDVVARGTGTQARLTRPTAGKTG
                     TADKAKDIWFVGFTPDMVTCVWGGGDEKKVVAKNVTGGTVMARIFRSFNQQYYSAVNT
                     PAGELLSSRYNDMDSPHEPVKPISHSNDVPLSEIDPSYAVSQSSAQQVRRRADPGQSA
                     VIRSQKGVTEYNWEP"
     gene            complement(2681..>3451)
                     /locus_tag="PKW73_05765"
     CDS             complement(2681..>3451)
                     /locus_tag="PKW73_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72821.1"
                     /translation="WLVIVLLCNSGRFSTVRESVENIAAGTMFGVQSRNYMDYLSHRF
                     VYDLNAPMTQEQAKVRVAQAEALLSLQVRYLGLRDYETARTMLNTAVLASRAGDVQKT
                     QEYVNAWLKLPVKRAERMLRKDEFLSVAQGKSAQLVAARLLVQAGSIDDGISQAQVCK
                     DSAAQKDDYKRYADSCEFLANEYEAKGDLANMLAQKRDLFRCKEVERKIYADDKGERA
                     ASDMFDADLRQLTLNIYDLAARVVTERQAQQQTKTTGR"
BASE COUNT          890 a         1004 c          825 g          732 t
ORIGIN      
        1 cttgatgcgc tccgaggcgc aatccttcaa gcaatatccc tgagccccgg ctgacaaagc
       61 cgcaaaaatc tcatcgtcat tgtcatggga ggtgagcatg acaatattca cagaaggcaa
      121 gttttgcttg atatctcggg tagcctcgat accgttctta taaggtagac cgatgtccat
      181 gacaattaca tcgggcttga gagtctgggc aagacgcacg gcttcctcac cgtcttccgc
      241 ctcaccgacc acgcaaaatt cctcagtgcg ctccaggaca gatctgagcc cctgccgcat
      301 cagttcgttg tcttcaacta tgagaacatt tacttttttc aaggctgcaa cctaccaaga
      361 ccacctaatt atagaacaaa catagaccct gtcgaaatgc cggcacaatt tgacagcccc
      421 tggagcctgt gccaggatag cccttaagga tagagcatgt cctgaattca ggcaggtgaa
      481 aactcaagaa aaatgtcgga tctgcgaaag ctgactggta gaacccattt cagggatact
      541 ctcacctctt tgtttcatat aacgaaagcg cttatccagc tgcttctggt tttcggaaca
      601 ctgggcggca cagtcatagg gctactggta gtgcaggaat tgatgcgcat accggatctc
      661 agcttcctaa aagactataa gcccgtcgac gccatttcta tctatgacaa aaatgacgaa
      721 ctcatagaaa ccatcgatgc cggcatccca cgcactgtga tcgcctacaa agatattccc
      781 cgagtactga tactggcagt gatggcagct gaggacaagc acttcttcga gcaccacggc
      841 gtcagccccc agggcatact gcgcgccacc ctggtaaatg cccgctcaat gcagatgaaa
      901 gagggcggtt cgaccatcac tcaacagttg gccaaaaacc tcttctttct agacggcaag
      961 cgcaccggta tcgtcaagct ggctgagatg ttcgtggccg gccgcctgga agagaagttt
     1021 tccaaagaag agatactctc gctttatttg acggaaattt actttgggga aggtgctcgc
     1081 ggaatagaac aagcagccca gacctatttc ggcaaatcag cccgtaacct caacctggag
     1141 gaagcagcat atctcgccgg tataattcgc tccccttcct atctgggctc ggccaagcac
     1201 cgtgacgccg ccttagaacg gcagcggcaa gtcttaagat ctatgcagga aagtaattac
     1261 atcaccatgg aagcggagat gagggccgag gccactcccc tcaactttcg caaaaccgcc
     1321 ctaaaacggc agaaaacata tcctttcaca cgctttccct actttacctc ttatgtgttg
     1381 gaactggttc gcaactccta cagcgacatg accatcggca tgaacggcat gaaaatttac
     1441 acgacccttg acccggtcgc tcaaagcctg gctgaaagaa ccctggtctc agagataaga
     1501 cgagcccctg tcggtataga agaagaagct ctggtagcac tctcggtcaa ggacaatgcc
     1561 attcgtgccc tcgtgggcgg cgcccacaat tactgggaca atcaatggaa ctgtgccgtc
     1621 aacccgcata cagccggctc gtcaatcaag ccctttgttt atctaactgc cttcatggcc
     1681 ggtgtagcca cgcccgagag cacggttctc gatgctccct tcgaactgaa ggaggcaaac
     1741 ggtgagaaat ggacccccaa gaactttgac ggcaagtacc tgggcaatat caccatccgg
     1801 gacgccctca tgcagtcgcg caatatgtgc accattcgcg tcaccgagca ggtaggaatc
     1861 aattccatca tttccaccat gaactcggcg ggcattcgca ccaagatggc tcccaccctg
     1921 gcactggccc tgggcagttc cgccgtcacc cccctggaac tggcctcagc ctacggcact
     1981 ctcgccagag gcggtctggc tactcccgcc tggaccattc gtcgtataga agatgcccgc
     2041 ggcaaaatcc tcagttacta tcagccgtcc atttgccgag tctttcccca ggaacaagtc
     2101 acctggctca cggacatttt gcaagacgtg gtggccaggg gcactggcac ccaggccaga
     2161 ctgacaagac cgacagcagg caaaaccggc accgccgaca aagcaaaaga catatggttt
     2221 gtgggcttca caccggatat ggtgacctgc gtctggggcg gcggcgacga gaagaaagtg
     2281 gtggctaaga atgtcaccgg cggtacagta atggcccgca ttttcagaag cttcaaccag
     2341 cagtattaca gtgcggtgaa cacaccggcc ggcgaactac tgagttctcg ctacaacgac
     2401 atggacagcc cccatgaacc ggtaaaacca atcagtcaca gcaatgacgt acccttgagt
     2461 gaaatagacc ccagctacgc agtcagccag agctcggcac agcaggtgag gcgtagagcc
     2521 gacccgggac aaagcgccgt gatacgcagc cagaaaggtg tcaccgaata caactgggaa
     2581 ccgtgagtag accggctggc aggcaggcaa gtcgagaaaa gtcacaacta ctgaaaccaa
     2641 gtgttcgtat tgccccagag cctgaacaaa acgataaaga ctaccgaccg gtggttttcg
     2701 tttgctgctg cgcctgcctt tcagtcacca ctctggcagc cagatcatag atattcagag
     2761 tcaactggcg caagtctgca tcaaacatat ctgaagcagc tcgctcgccc ttgtcgtcag
     2821 cgtaaatctt ccgctccact tccttacagc gaaacaggtc gcgcttctgc gccagcatgt
     2881 tagccagatc gcctttagcc tcatactcat tagcaagaaa ttcacagcta tcggcatagc
     2941 gcttataatc gtccttctgc gccgcactat ccttgcatac ctgagcctga cttatcccat
     3001 catcgatact accggcctgc accagaagtc tagcggcgac cagctgcgca gacttgcctt
     3061 gcgccacaga gagaaactcg tctttgcgaa gcatgcgctc ggctcttttt acgggcagtt
     3121 tcaaccaggc attaacatac tcttgggtct tttgcacgtc tcccgcccgt gaggctaata
     3181 ccgctgtgtt aagcatggtc ctcgccgtct cataatcgcg caaccccaga taacgcacct
     3241 gtagcgaaag caaggcttcc gcctgggcaa ctctcacttt cgcctgttcc tgggtcatcg
     3301 gtgcattcaa gtcgtaaaca aaacgatggg aaagatagtc catgtagttt cgtgattgca
     3361 caccaaacat agttccagca gcaatgttct cgactgattc tcgcaccgtc gagaaacgac
     3421 cactgttgca gagtaaaaca attaccagcc a
//
LOCUS       DAOQNZ010000251         3450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13348_length_3450_cov_3.673049, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000251 DAOQNZ010000000
VERSION     DAOQNZ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13348_length_3450_cov_3.673049"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKW73_05770"
     CDS             <1..251
                     /locus_tag="PKW73_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72822.1"
                     /translation="MLKSRVKAYKNTAGRGTYKGVVMLEEAEFSRLCLCADQALARPT
                     DMKAASKAVNDVHGRLRSLEQWEAEDKGSNQTSKSESV"
     gene            248..2641
                     /locus_tag="PKW73_05775"
     CDS             248..2641
                     /locus_tag="PKW73_05775"
                     /inference="COORDINATES: protein motif:HMM:NF028228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72823.1"
                     /translation="MTMQENICSCGTEYGADGICPDCGNIKPEAVKALDASQGNLVPD
                     VLSVHSGHTKNTGLSSRTSKSSRARQLALSLRTQVTARPVTVVAIVDESGKADGKVRK
                     RRHEFSTRTSTRTTRGIAAGLVELETLPPVDAQSLIREGLGISKEQRVCGNPECQHLQ
                     AHGEPRNLYREKNGVPIDKGLCPSCRAPFNFSPIPKETVISGQYSVLGPIAVGGFGFI
                     YLAWDMNVGRYVVLKGLLNSRDPEQQILAVQERRFLADLQSPEIVNIVNFVTHSEHHD
                     ESQDGQEVDMQTFIVMEFIDGQTLKSIKKKWMQENANAPLPVTDAIYFMLGILPAFTY
                     LHKQGVYYCDFKMENAMLQGDRLRLIDLGGARKASEVGGSCFLTRGYAAPEAGGDNPE
                     PSVSSDLYTVGRTLAILCTNFDWLEEFEFDLPTPDREPLFARYESFYRFLLRACHRDP
                     DQRFQSALEMSEQLLGVLAEIVSIDTGNARPVFASPEFALDVLREVGENSKPSYLSLT
                     DLKPDRTDSANALIETALAENDLAQQAKLLKQAMEQFPRSAEAPLRLAMVATALKDYS
                     WAETIIAERIVVDPYDFRNIWVKGAWLMAQGRYADAFKHFDAVYSEVPGELAPKLALA
                     LCAELNGDTDVALRYYELVSGIDPSFVTAVFGLARCLYTKDAVAAADAFDRVSPLSAS
                     FQQALASKMQLLIKGKPNAELLTMAVATMKRINLDGYAYHQLRFQLFEALLAAVEDNR
                     LPVGEVANLVQFAGAGTDYLRLAMEQEARFCAHSCGEQELSIQWIDRANAVRPLTRF"
     gene            2655..>3450
                     /locus_tag="PKW73_05780"
     CDS             2655..>3450
                     /locus_tag="PKW73_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72824.1"
                     /translation="MNCPKCLEPVEEGDLFCVCGADLACASEKSETKNGASAAKGDAA
                     KSGSAGGLFKPPGMGAAAKNTGKKKGGLFSVPGAAVPVSRSVEKPVKSTAVKVKGRPC
                     PDCGADSEQVSSDGICTNCGTQVVFSQRDDFTVAASNELAVRSFLGRCHHRNEDYALA
                     ADIVLEPSGKTYTVILVSDGVSSSECAHLASEAACTRGMEFLAQALKSGVKADCDLLA
                     EAICAAQEAVLAVKGNGETDKLGRAKVPPEATFLAILLDGEKAYLGW"
BASE COUNT          743 a          919 c          993 g          795 t
ORIGIN      
        1 aaatgctcaa gagtcgcgtc aaagcttaca aaaatacagc cggtcgaggc acctacaaag
       61 gtgtggtcat gctggaggaa gctgaattta gcaggctttg cctgtgcgcc gatcaagctc
      121 tggctcgccc caccgacatg aaggcggcca gcaaagccgt caacgacgta cacggtcgcc
      181 tgcgcagcct ggagcaatgg gaggcggagg acaaaggtag taaccaaact tcaaagagtg
      241 agagcgtatg actatgcaag aaaatatttg ttcctgtggt accgaatatg gtgcagatgg
      301 tatctgcccg gattgcggta atatcaagcc ggaggccgtt aaagcccttg atgcctctca
      361 gggcaacctg gtcccagacg tgctttccgt tcattccggt catactaaaa ataccgggct
      421 ttcctcacgt acaagcaagt catcccgggc aaggcagctg gctctttcgc tgcgtactca
      481 ggtgacggcg cgtccggtca ccgtggtggc cattgtggac gagagcggca aagccgacgg
      541 aaaggttcgt aagcggaggc atgaattctc cacccgtact tccactcgta ccacccgcgg
      601 tattgccgcc ggtctggtgg aattggaaac tttgccgccg gttgacgcac agagcctgat
      661 tcgagaaggt ctggggatca gcaaggagca gcgggtctgt ggtaatcccg agtgtcagca
      721 tctgcaagct cacggtgagc ctcgcaatct ctatcgcgag aagaatggcg ttcccatcga
      781 taaagggctc tgtccttcct gccgcgctcc cttcaatttc tcgccgattc ccaaggaaac
      841 ggtcatttcc ggtcaatact cggttctcgg accgatagcg gtaggaggtt tcggcttcat
      901 ctatctggcc tgggatatga acgtggggcg ctacgtcgtc ttaaaaggac tgctcaattc
      961 cagagacccg gagcagcaga ttctggctgt gcaggaaagg cgattcctgg ctgatttaca
     1021 gtcaccggag atagtcaaca ttgtcaactt cgtcacccat tccgagcatc acgatgagag
     1081 ccaggatggt caggaagttg atatgcagac ctttatagtc atggaattca ttgacggaca
     1141 gaccctcaag tcaatcaaga aaaagtggat gcaggagaat gccaatgcgc cgctgcctgt
     1201 caccgatgcc atttacttca tgctcgggat tctgccggcc tttacctact tgcataagca
     1261 gggtgtttac tactgcgatt tcaagatgga aaatgccatg ttgcagggtg accgtttgcg
     1321 gctcatcgac cttggtggtg ccagaaaggc ttcggaagtg ggcggcagtt gcttcctcac
     1381 tcgcggctat gcggcaccgg aagcgggcgg cgataatcct gaaccgtcgg tttcctccga
     1441 cctctacacc gtaggacgca ctctggccat cctctgtacc aattttgact ggctggagga
     1501 attcgaattc gatttgccca cgccggatcg agaaccgctc tttgcgcgct acgagtcgtt
     1561 ctatcgcttc ttgctgcgcg cctgccaccg cgacccggac cagcgttttc agtcggctct
     1621 ggagatgtcc gagcaacttc tgggagtgct ggctgagatt gtttccatcg acaccggcaa
     1681 tgcccgtccg gtttttgcca gccccgagtt tgctctcgat gtattgcgcg aagtgggtga
     1741 aaacagtaag ccgagctatc tgtctttaac tgatttgaag ccggaccgga ctgattcagc
     1801 caacgcgctc attgaaacgg ctctggccga aaacgatctg gcgcaacagg ctaaacttct
     1861 caagcaggct atggagcaat tcccccgttc agcggaagcg cctttgcgtc tggccatggt
     1921 ggctaccgcc ttgaaagact atagttgggc ggagacaatc attgccgagc gcattgtggt
     1981 tgacccttat gacttccgca atatctgggt taagggtgcc tggcttatgg ctcagggccg
     2041 ctacgcggat gccttcaaac acttcgatgc cgtttattcc gaggttccgg gcgaactggc
     2101 tcccaagctg gctcttgctc tctgtgccga gctgaacggt gatacggatg tggctttgcg
     2161 ctactacgaa ctggtgtccg gcattgaccc cagctttgtc acagccgtat tcggtctggc
     2221 tcgctgtctc tataccaaag atgcagtggc ggctgccgat gcctttgatc gcgtttcacc
     2281 cctttccgct tccttccagc aagctctggc cagcaagatg cagctcctca tcaagggcaa
     2341 gccgaacgcc gagcttttga cgatggcggt tgccaccatg aagcgcatca atctggatgg
     2401 ctacgcttat catcaattgc gcttccagct cttcgaggcc cttctggcag cggttgaaga
     2461 taaccgcctg ccggtgggcg aagtggccaa cctcgtgcaa tttgcaggcg ccggcaccga
     2521 ctatttgcgt ctggcaatgg agcaggaagc tcgcttttgt gctcatagct gcggtgagca
     2581 agaactttcc attcagtgga tcgacagggc taatgccgtg cggccgctga cccgtttcta
     2641 aatggggagt aaacatgaac tgtccaaaat gtttggagcc cgtcgaagag ggcgatttgt
     2701 tttgtgtctg cggtgccgat ctggcttgcg cctctgagaa gagcgaaacc aagaacggtg
     2761 cttccgccgc taagggcgat gccgctaaaa gtgggtctgc cggcggcctg tttaaaccac
     2821 ccggcatggg tgctgcagcc aagaatacag gcaagaaaaa gggcggcttg ttcagcgttc
     2881 ccggtgcggc ggtgccggtc tccaggtctg tggaaaaacc ggttaagagt accgccgtta
     2941 aggtcaaagg gagaccctgc ccggattgcg gtgccgattc ggagcaagtt tccagtgatg
     3001 gaatatgcac caattgcggt acccaggtgg tgttcagtca gcgtgacgac tttacggtgg
     3061 ccgcgtcaaa cgagcttgct gtgagaagtt ttctggggcg ttgccatcat cgaaacgaag
     3121 actatgctct ggccgcagat attgtgcttg agcccagcgg taagacatat acggtgatac
     3181 tcgtctccga cggtgtatca tcttccgaat gtgctcatct ggcctcggaa gcggcttgca
     3241 ccagaggcat ggagtttctg gcgcaggccc ttaaaagtgg tgtaaaggcc gactgtgatt
     3301 tgttggctga agccatttgt gccgcccagg aggccgtact cgccgtcaaa ggaaatggtg
     3361 aaaccgacaa actcggccgc gccaaagtgc cgccggaagc cacttttctg gcaattctgt
     3421 tggacggaga gaaagcctat ctgggctggg
//
LOCUS       DAOQNZ010000252         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13370_length_3448_cov_3.723843, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000252 DAOQNZ010000000
VERSION     DAOQNZ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13370_length_3448_cov_3.723843"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKW73_05785"
     CDS             complement(<1..454)
                     /locus_tag="PKW73_05785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA72825.1"
                     /translation="MKQEPQVKEVTPYNSEQAKFEFLVERISTRVRGKIFESFKRLVP
                     PTRELKVLDVGVTCDRRADSNFFELHYPYKDRITAVGLENAAFLEEIYPGLTYVKADA
                     LDLPFEDDSFDVVVSWAVIEHVGSRERQRRFINELSRVGKKIFVTPPNR"
     gene            990..2192
                     /locus_tag="PKW73_05790"
     CDS             990..2192
                     /locus_tag="PKW73_05790"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA72826.1"
                     /translation="MLAPSRPTLEKDSIKRVRRSSRKTGRRLLAFTASLLWLGSLSQW
                     APAAASDDDGADPRWGYISPSGDYAIKPRFMAAGEFREGLAAVKVNVSAKAKAAAGGE
                     QSRDDQDEQSEKSEEADNGEAKWGFIDSSGKLVIQPQFEEVLAFSEGLAAARQGKWGF
                     IDKSGKFVVEPQFEDVSSFSEGLAPASRSSLKWGFIDRTGTWKIEPNFQTVCRFSDNR
                     AAVMTGDGFGSYQFKVSEDRYEVRGAHWSYIDKSGKTVIDSQFEAAGLFSQNLAPVAL
                     GYKQGVNRPDRWGFLNTDGKLSIKPQFQDVHAPSQDTAAVQFGTWQNIGKGYRSWIPG
                     KWGYIKLTGKTLIPPQFDAADRFSEGLASVYLSGKWGYIDKAGKLVIAPKFKYTGEFH
                     EHLAPVCP"
     gene            2402..2944
                     /locus_tag="PKW73_05795"
     CDS             2402..2944
                     /locus_tag="PKW73_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72827.1"
                     /translation="MASELEKEDDPLFAMPMLFNCPMKWQKMSGDERIRFCQSCRQNV
                     YNLSAMTKEEALALIEEKEGKLCVRFYRRKDGMVITKDCASYWGKSIRERNSALAVIN
                     ATLAFMANILAPILGPSQITLVVGNSPLMARQSPEEKLAEQLAEQREANRKQAEEAAQ
                     AAQELQELQERKQRAVWPPR"
     gene            3204..>3448
                     /locus_tag="PKW73_05800"
     CDS             3204..>3448
                     /locus_tag="PKW73_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72828.1"
                     /translation="MMSRFNCRLVLLTLVSLTSLSSAEAQNAQSLAYLAPNSSLGSTT
                     TLDTERSKAQNLYQSGQFAQALLLYHQICSGRSNPPTA"
BASE COUNT          934 a          942 c          869 g          703 t
ORIGIN      
        1 agcggttggg gggggtgaca aagatcttct tacccacccg ggaaagctca ttgataaagc
       61 gcctttgccg ttctcttgag cccacatgct caataaccgc ccagcttaca actacatcga
      121 aagaatcatc ttcaaagggt aaatccaggg catcggcttt gacataggtc aggccgggat
      181 agatttcctc caggaaggcc gcattctcaa gcccaacggc ggtaatgcga tccttataag
      241 gatagtgcaa ttcgaaaaaa ttagaatcgg ctcgacggtc acaagttacg cccacgtcca
      301 gcacctttaa ctcgcgggta ggcggcacca ggcgcttgaa agactcaaaa atcttgcccc
      361 gcaccctggt agaaattcgt tccaccagga attcaaactt ggcctgttcc gagttatagg
      421 gcgtaacctc tttcacttga ggctcttgct tcatgtccac caaccgacca tcctcacacg
      481 tccgcaatta ttatcgcaat tcacatcgca actgactgtc cgtaccaggg cagcgacaag
      541 aatacgccgc cgctccgggg ccggtcccta tagttttcca caactgaacg ccaatagcac
      601 acttggcagc caatgagagc ccgagaagct tgattttctc atggaggcta ataaaaacaa
      661 cctggttcgg ttggtgggct gtcgctctta ctacttttta tagttctatc ttagccagta
      721 gtacagaggc gagcgcgcga gcctgggagg cggcctcaca agcccagaga tgtgccggaa
      781 ataccagaat ccgcaaaagc ctgtcaaagt ccgcaagtaa ctgccagtaa acaccgggaa
      841 atctcaagta gccataaaac cgcctaaatc gatcggctcg gcagtggagc aagagctaga
      901 ctccacagag ccggccaata actccaagaa tgaagaagta agtaaagcaa gctcactgaa
      961 actgcactga aaggacaagg aatttcaaga tgctcgctcc ctccagacca accctagaga
     1021 aagactcaat aaagcgcgtg cgccgctcca gccgcaaaac cggcaggcgg ctgcttgcct
     1081 ttacagcttc cctgctatgg ttgggtagtc tcagccaatg ggcaccggcc gcggcctcgg
     1141 atgatgatgg tgcagacccg cgctggggct atatctctcc cagcggcgac tatgccatca
     1201 agccccggtt catggcggcg ggagagttcc gtgaaggtct ggcagccgta aaagtaaatg
     1261 tctcagcgaa ggcaaaagca gccgccggcg gagagcaatc cagggacgat caagatgagc
     1321 agtcagaaaa gagcgaagaa gccgacaacg gcgaagcgaa gtggggcttt atagatagct
     1381 caggtaaact cgtcattcaa ccacagtttg aagaagtgct ggccttttct gaaggactgg
     1441 cagcagccag acaaggcaaa tggggcttca tcgacaagag cggtaaattc gtcgttgaac
     1501 cacagttcga agacgtttcc agtttctcgg aagggctggc accggcaagc cggtccagcc
     1561 tcaagtgggg ctttatcgat cgcaccggca cctggaagat tgaaccgaac tttcaaactg
     1621 tctgtcgctt cagcgacaac agagcagccg taatgacagg cgacggcttc ggttcttacc
     1681 agttcaaagt gagtgaagac cgttacgaag tgcgcggcgc gcactggagt tatatcgaca
     1741 aaagcggcaa gaccgttatc gatagtcaat tcgaagcagc cggtctattc tcgcaaaatc
     1801 tagcgccggt ggccctgggt tacaaacagg gtgtaaatcg cccggaccgc tggggctttc
     1861 tgaacactga cggcaaactc tctatcaaac cgcaattcca agatgtgcac gctccatcgc
     1921 aagacacagc agccgtgcag ttcggaacct ggcaaaatat cggtaaaggc taccgttcgt
     1981 ggattcccgg caaatggggc tatatcaaac tgaccggcaa gacactgatt ccaccgcaat
     2041 tcgacgctgc cgaccgtttc tcggaaggac tggccagtgt gtacctatca ggcaaatggg
     2101 gctacatcga caaagccggc aagcttgtca ttgctcccaa attcaaatac acaggcgaat
     2161 ttcacgagca tctggcgccg gtctgcccct gatcaatctg cttctaaatg agactgttta
     2221 taaactagat gggcacgcaa cgcggcctcc atatcacttt gtaaatttcg aaagctcgtc
     2281 ggttagccat cttgaaacac cgggcaaaac aaactaggtt ttttgaactg ctattcaggc
     2341 acgcctgaat tcttctgcgc cgggccccgg aaacccttct gagactggga tgaggttgac
     2401 tatggcatcg gaattggaga aggaagatga ccctcttttt gcgatgccga tgctcttcaa
     2461 ttgccccatg aaatggcaaa aaatgagcgg cgatgagcga attcgattct gccagtcctg
     2521 ccgacaaaat gtctacaacc tttcggcaat gacaaaggaa gaggccctcg ctctcataga
     2581 agagaaagaa ggcaagctat gcgtgcgctt ctaccggcgc aaagacggaa tggtcataac
     2641 gaaagactgt gcatcatact ggggcaaaag tatcagggaa agaaacagcg cccttgccgt
     2701 gatcaatgcc acgctcgctt tcatggccaa tatactggcc cccatcctcg gtcccagcca
     2761 gattacactc gttgtgggca actctccact tatggctcgt caaagcccgg aagaaaagct
     2821 ggctgagcag ctggcagagc agcgggaagc caacagaaaa caggcggaag aagcggcgca
     2881 agccgctcaa gaattgcaag agctgcaaga aaggaaacaa cgagcagtct ggccgcccag
     2941 ataaaagtca gagcaccttc ccctcttgac acgaagggct tgccagcagc gtatttcccg
     3001 caggaattgt ccgcttttgg tcacgcgtct gtaagaagat ttgcagcgaa aagtactgca
     3061 cttctaaagt gcaatatgcc attgggctga aattaaggga acaaggtaaa ccgcttacaa
     3121 ccgagttgga agccgggcgg tacaaccgtg accgcaattg tttcttctag ccctggcaaa
     3181 ccataaagca gggagtcatt cttatgatga gcagatttaa ctgccgcctc gtcttactta
     3241 cacttgtcag cctgacctcc ctgtcgtccg ctgaagccca aaatgcgcag tctcttgctt
     3301 atcttgcccc aaacagcagc ctcggcagta ccaccacact cgataccgaa cgcagcaaag
     3361 cgcaaaacct ctatcagagc gggcaattcg ctcaagctct ccttctttat caccagatct
     3421 gctcggggcg ctccaacccg cctacagc
//
LOCUS       DAOQNZ010000253         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13397_length_3444_cov_3.154618, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000253 DAOQNZ010000000
VERSION     DAOQNZ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13397_length_3444_cov_3.154618"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKW73_05805"
     CDS             <1..354
                     /locus_tag="PKW73_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72829.1"
                     /translation="DPHALKPGMVLKIEGTRTGRHKDENGCEQRNYESTHGQKVAGAC
                     DTVTALPGAKVVLHQGSEATVTRGATGFGFPGSKARVLAGGRFIAAGGEVQVEDGAMV
                     MQAAPSEIVAMKTPN"
     gene            380..1210
                     /locus_tag="PKW73_05810"
     CDS             380..1210
                     /locus_tag="PKW73_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72830.1"
                     /translation="MSSLEHSEIDSKAGSPAQWTQDAAHQAWSGDQNRNTGKSLLQEP
                     QSLDFTPVANGGKGSVEPHACDTPEKPPEKPPEKPPTEPPPEKPPEKPPTEPPPEKPP
                     EKPPTEPHCTVDCGPPPPPPPPPEKPQEPSRNVNTANADANANAAGGKVNMVTRQGAA
                     TMPVLTSTSECAEARAFGINLFVIGVGAGEQWLNQTCEQIKTVKAVCGIANENTSLAI
                     EALKIAGSVNENRFEQRLATRISHDKAWDGRRVNANCAVEKTKLENMLAEQREKTEED
                     "
     gene            1367..2047
                     /locus_tag="PKW73_05815"
     CDS             1367..2047
                     /locus_tag="PKW73_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA72831.1"
                     /translation="MSKILLVEDDLDLAKSVAIWFEDEDHIVEHVSDGKEAINRICTA
                     SYDAIILDWELPGTSGLEVCRNIRDQGINVPIIMVTGHGEIDSRITGLDVGVDDYLTK
                     PFSLKELAARIRALIRRSAGSASPVLKAGNIELDPRSHLVTKSGAPIQLMPREFAVLE
                     FFLRHKNQIFSTEALLQRLWHTDSDSSPEAVRACVKRLRKKIDDEGLEDSKSMIETIP
                     KLGYRLNG"
     gene            2096..3079
                     /locus_tag="PKW73_05820"
     CDS             2096..3079
                     /locus_tag="PKW73_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNA72832.1"
                     /translation="MKALCLDKPGPIESLYLTDREEPTPKSDEIRVQVRAVGLNPVDY
                     KIARTGHPAWQFPFILGLDVAGIIDSVGENVRELSPGQRVVYHGDLSKPGGFAQYAAT
                     NARAVSVIPEQLSFTDAAALPCAGYTAFQAIHKKLKVVSGKTILIHGGAGGVGGFALQ
                     LSRLANLKVFTTCSRRNFEYVMSLGADQAIDYNTEDVAAQIKAATGGRGVDYIVDTAG
                     PESATASLAMLPFGGALVCCAGFPDFSKIIPFKSALSIHEVALGGAHLSGDHQAQHEL
                     AHIGTKMLELAVSGSLNCLVEEIISLEEIPVGLKKLAERHARGKIVATVGD"
BASE COUNT          941 a          950 c          897 g          656 t
ORIGIN      
        1 gatccccatg ccctcaagcc tggcatggta ttgaaaatcg aaggcaccag aaccggccgc
       61 cacaaagacg aaaatggctg cgaacagagg aactacgaaa gcacccacgg tcaaaaagtt
      121 gccggtgcct gcgataccgt cacagcactg ccgggagcaa aagtggtgct gcaccagggc
      181 tcagaagcca cagtcacccg tggtgcaacg ggcttcggct ttccaggcag caaagcacga
      241 gtgcttgccg gcggcaggtt tattgcagcc ggaggagagg tgcaagtgga agatggtgcc
      301 atggtcatgc aagcggcacc gtcagagatc gtggcaatga aaacccctaa ttaagacaaa
      361 gcgcccgagg gaccctcata tgagttcttt agaacattca gagattgatt cgaaagcagg
      421 aagccctgcc caatggacac aagacgccgc ccatcaagcg tggtccggcg atcagaaccg
      481 aaacaccggc aaaagcctgc ttcaagagcc tcagtcccta gactttacac ctgtggcaaa
      541 cggcgggaag ggctctgttg agccacacgc ttgcgataca ccggaaaaac cgccggaaaa
      601 gcccccggag aaacctccta ctgagcctcc gccggaaaag cctccggaga aacctccgac
      661 cgagcctccg ccggaaaagc ccccggagaa acctcccacc gagcctcact gcacggtgga
      721 ttgcggacca ccgccaccac caccgccgcc gcctgaaaag ccacaggaac catccagaaa
      781 cgtcaacact gcaaacgccg atgccaacgc caatgccgcg ggcggcaaag taaacatggt
      841 cacgaggcaa ggtgcggcca cgatgccggt cttgaccagc acaagcgagt gtgcagaagc
      901 tagagcattt ggcatcaatc tgtttgtcat cggagtagga gcgggagaac aatggctcaa
      961 tcaaacctgc gaacagatca aaaccgtaaa ggcggtttgc ggcatcgcca atgagaatac
     1021 ctctcttgca attgaagccc tgaaaatagc cggctctgtc aatgaaaatc gcttcgaaca
     1081 gagactagcc accagaatat cccatgataa ggcctgggat ggacgtaggg tcaacgccaa
     1141 ctgcgcagtg gaaaaaacca aacttgaaaa catgctggca gagcaaagag aaaagactga
     1201 ggaggactga gactaataag gaatggacag cggttaacaa gatgcgacga cttaaacaga
     1261 cgtaacggta agtcacatcc gttgacaatg atgcatcttg attgccactg actcaatgcc
     1321 tcagtagaat agaggagaag acccagcgga aatatcctcg gcattcatgt ccaaaatcct
     1381 attagtcgaa gacgatttag accttgccaa atcggtggca atctggtttg aagacgagga
     1441 tcatatagtt gaacacgtta gtgacggaaa agaagccatc aaccgcattt gcactgccag
     1501 ctacgacgcc atcattctcg actgggaatt acccggcaca agcggtctgg aagtgtgccg
     1561 caatattcgc gaccagggca tcaacgtacc gattatcatg gttaccggtc atggcgaaat
     1621 agacagccgt atcaccggac tcgatgtggg agtggacgac tacctgacca agcctttcag
     1681 tttgaaagag ttggcggctc gcatccgggc cctgatccgc cgctctgccg gcagcgccag
     1741 cccagtcttg aaagcgggca atatagaact cgatccgcgc agccacctcg tcaccaaatc
     1801 cggtgcacca atccagctca tgcccagaga atttgccgtg cttgagtttt tcctcagaca
     1861 caaaaatcaa atcttcagca ccgaagctct gctacaaaga ctctggcata ctgactccga
     1921 ttcttcaccg gaagcagtga gagcctgcgt caaacgtctg cgcaagaaaa tcgacgatga
     1981 aggtctggaa gattctaagt ctatgattga aacgattccc aagcttggtt atcgtctcaa
     2041 tggttgaagc cggtataaat tctctgcctt gactataaag taggaaggaa acaagttgaa
     2101 agctctttgc ctcgacaagc cgggccctat cgaatcgctc tacctgactg accgggaaga
     2161 gccgacacca aagtccgacg aaattcgagt ccaggtgaga gcagtcggac tcaatcccgt
     2221 cgactacaaa attgctcgta caggtcatcc cgcctggcaa tttcccttta tattaggtct
     2281 cgatgtagcg ggcataatcg acagcgtcgg cgaaaatgtc agagaactca gccccggtca
     2341 gcgagtggtc taccacggtg acctttcaaa accagggggc tttgcccaat acgccgcgac
     2401 aaacgcccgc gccgtttcgg ttatacccga acaattgtcc ttcaccgatg ccgccgccct
     2461 accttgcgca ggttatacgg cttttcaagc cattcacaaa aaattgaagg tggtgagcgg
     2521 caagactatt ctcattcatg gtggcgccgg cggtgtgggc ggcttcgctc tgcaactgtc
     2581 cagactggcc aatttgaaag ttttcaccac ttgctccagg cgcaattttg aatacgtcat
     2641 gagcctgggt gccgaccagg ccatagacta caacacggaa gacgtagcgg ctcagatcaa
     2701 agcagctacc ggcggcaggg gggtggatta tatagtggat acagccggtc cagaaagcgc
     2761 cacagcctca ctggcaatgc tgccttttgg cggtgctcta gtgtgctgtg ccgggtttcc
     2821 cgatttcagc aaaattatcc ccttcaagtc ggctttatct attcacgaag tagccctggg
     2881 cggcgcccat ttgagcggcg atcaccaggc tcagcatgag ctggcacaca taggcacgaa
     2941 aatgctggaa cttgcagtat caggcagttt gaactgcctg gtagaagaga taatcagcct
     3001 tgaggaaatc ccggtcggtc tcaagaagct ggcagagcgc catgcccgcg gcaaaattgt
     3061 agccacagtg ggagattaat acggcgcggg aacattaact caagccagat gtctaactta
     3121 aagtagccga aaacggcaag cagtatacat ggctttgaag aggatgtgac cgtgagtgga
     3181 aacgtcagga ctactgcctt aaagccaaac cccaagagtt atgcggcctt gctcgcagcg
     3241 ccggccatct tggtgccgct ctgctcagtt tctccctgcg aagcgcagct tcaatcggac
     3301 gccccatact ttacagatct ccctctgccc ggtcgacacg accttatttg gaactacgac
     3361 ccaagtaaac cgctgctcgg gccaaacagt gacatagaca aacttatgag agacgggggg
     3421 catcgcccct atccggcgac cagg
//
LOCUS       DAOQNZ010000254         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13484_length_3428_cov_4.184702, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000254 DAOQNZ010000000
VERSION     DAOQNZ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13484_length_3428_cov_4.184702"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKW73_05825"
     CDS             <1..773
                     /locus_tag="PKW73_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72833.1"
                     /translation="ALTAQVLVRDGKISPEQAIQALQSSRFRRVNIEVSLMDLGFYKP
                     PPRQRVKIGELLVLSGLVNESDLMTALELGLIREAPVGQMLVEGGFISKRILDAALKL
                     QELVSGHSLSALKASEALRRVAVNHVSLARAISELDLESSERKERVRLGEMLKACGIV
                     GDTDIKKALKDASRNSALLGKVLLVTGVIDEHTLHAALRLLLLLRSEKLTMEQAIMAL
                     NHARKHKLTVDEALQELKWSVPEEADIENEPEPEAGST"
     gene            complement(864..>3428)
                     /locus_tag="PKW73_05830"
     CDS             complement(864..>3428)
                     /locus_tag="PKW73_05830"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNA72834.1"
                     /translation="QYGIVLTIPIGNYVVWQRLKQRNLANVLRIGLLNGLSAGAAGLV
                     LVGCVAACALGYPAIDSNLISHQSEYAICAMLTFCSLVASLRLMQMLCLIWETDGART
                     SQLIYSLAGVMLSLLAVGASEARQVAVRVNEKAALTEDSDKRTEALRNLRNPLLDSTQ
                     ELKRDIAAPRTGGLSGLFLGVNDSSSRQLYFALTSQPYEAITDLAVDDQSNDTLTSSS
                     YDNFLARQVVGEAIKGFSLKRSQLSGLINARSLTANMSWTFVLKNASSTAQEARAEIA
                     LPPGAVVSNMTLWIDGKPVDATITSTSQARGAYTEVVTGRRDPALVTYLGKGRVLVQC
                     YPVMPGKETKVSLSMAAPLKLDAGTKSSLTLPRLVASNFAAGKSHSLRFNSPEELSLA
                     AAGLKEMKSLSSSHLYVGEMKEQDQKNTGLSLSASRPMTLGAFAAQDKVYGGYAVETV
                     KEVANQAPKNLVVVIDGSSKVKEYKQDLTEMLKRMAKMMPTTVLLADDDKLEGPEAVS
                     VENAIKKLNESSFEGGHDNLPAVVKAAELAGDQERPAVLWVHGPQPAFNEEIYITTQG
                     ASKPAYYELALDDGWTNSSQFFKNHRDIGPMTPVLRSGNLASDLNRFLDQWQPGGHHY
                     SVQLFKMNEKPACNILTGQEAQDLSTLVAAQTCKQLLTNRNLSAAGTLAVNYHIVSPL
                     TSGVVLENQSDYQRWGIQQNGPGRQNRQNNEAARLANGDSLSGFGTAEQTATGAIAIT
                     TSGDAFGSSPVTQGATNGTIAPQGVESSVVQGVNTAGTVRVNNLADIEALLNVLANGI
                     AIIGSILGLTTIVKACLRGFQSKSFVMGLSMIVIGLASPGVINWLVATARDSALFN"
BASE COUNT          684 a          914 c          981 g          849 t
ORIGIN      
        1 ccgcactcac cgcgcaggtg ctggtacggg acggcaaaat ctcaccggag caagccattc
       61 aggctttgca gagcagccgc ttccggcgcg taaatatcga agtttccctg atggacctgg
      121 gcttctacaa gccgccgccc cgtcagagag tcaaaattgg agaacttctg gttttatcag
      181 gtctcgtcaa tgaatcggat ttgatgacgg cccttgaact gggacttata agggaagcgc
      241 cggtgggtca gatgctcgta gagggaggct ttatctccaa gcgtatattg gacgccgcct
      301 tgaaactgca agaactggtt tccggtcatt ccttgagtgc cctgaaagca tcggaagcct
      361 tgaggcgggt ggcggtcaac cacgtatcgc tggcccgggc catatccgag ttagacctgg
      421 aatcgagcga gaggaaggaa cgggttcgtc tgggtgagat gctcaaagcc tgcggtatcg
      481 ttggtgatac cgatatcaag aaagccctca aagatgccag tcgtaattcc gccctactgg
      541 gcaaagtttt gctggtcaca ggcgtcatcg acgaacacac cctgcatgcc gctctcagac
      601 tcttgcttct tctgcgcagc gaaaagctga ccatggaaca ggccattatg gctctcaatc
      661 atgcccgcaa gcacaagttg actgtcgatg aggctctgca ggagctgaag tggagcgtac
      721 cggaggaggc tgatattgag aatgagccgg agcctgaagc tggttcgacc tagcagcata
      781 tttagagtca tctgaaagca aaagaccgcc gcggttatcc gggcggtctt tcacttcctt
      841 tttgtgattt tgtttcggcg ctcttagttg aacagggctg aatcacgggc ggtggctacc
      901 aaccagttga tcactcccgg tgaagccaga ccaatgacaa tcatggaaag acccatcacg
      961 aaggacttcg attggaagcc gcgcaggcag gcctttacta ttgtggtcag tcccagaatc
     1021 gaaccaatta tggctatacc attggccagt acgttcaaga gtgcttcaat atcagccagg
     1081 ttgttgaccc tgaccgtacc ggccgtgtta acgccctgca ctacactact ttccacgccc
     1141 tgaggcgcaa tggtgccgtt tgtggcgccc tgcgtaacag gagaggagcc gaaggcgtca
     1201 ccggacgtgg ttatggcgat agctccagtt gcagtctgtt cggccgtgcc aaagccgctc
     1261 aaggaatctc cgtttgccag tctggccgct tcgttgttct gtctgttctg cctgcccggc
     1321 ccgttctgct ggatgcccca gcgctgatag tcgctctgat tttccagtac cactccggaa
     1381 gttagcggtg aaactatatg gtagttcacg gccagtgttc ctgctgccga caaattgcga
     1441 ttggtcagca gttgtttgca ggtttgggcc gccaccagtg tggataaatc ctgggcttcc
     1501 tgtccggtga gaatgttgca ggccggcttt tcgttcattt tgaagagctg gactgaataa
     1561 tgatgcccac cgggttgcca ctgatccagg aagcgattga ggtcgcttgc cagattgccg
     1621 ctgcgcagca ccggcgtcat gggacctatg tctctgtggt tcttgaagaa ctggctgcta
     1681 ttggtccagc cgtcgtccag ggccagctca tagtaggccg gtttactggc tccctgggtg
     1741 gttatataaa tctcctcgtt gaaggccggc tgtggtccat gcacccagag tacggccggt
     1801 ctttcttgat cccctgcgag ttcggctgct ttcaccactg ccggcagatt gtcgtgtccg
     1861 ccttcgaagc tgctttcatt gagctttttg atggcatttt cgaccgatac agcttccgga
     1921 ccctcgagct tatcgtcgtc ggccaagagt actgtggtgg gcatcatctt ggccatgcgc
     1981 ttgagcattt cggtgaggtc ttgcttgtat tcctttacct tactggagcc gtcgattacc
     2041 accaccagat tcttgggggc ctggttggct acctctttca ccgtttccac ggcatagccg
     2101 ccgtaaactt tgtcttgcgc ggcaaaggct cccagggtca tgggtcttga ggcagagagg
     2161 gaaagtccgg tgtttttttg atcctgctct ttcatttctc ccacatagag atggctgctg
     2221 ctgaggctct tcatttcctt tagacctgcg gctgccagac tcaactcttc cggggagttg
     2281 aagcgaagac tgtggctctt gccggcagcg aagttgctgg ccacaaggcg gggcagggtg
     2341 agggaagatt tggttccggc gtccagtttg agcggtgcgg ccatagacag actgaccttg
     2401 gtctctttgc ccggcatgac cggataacat tgaaccagga ctctcccctt acctagatag
     2461 gtgactaggg ccggatctcg tcttccggtc accacttccg tataggctcc tcgggcctgg
     2521 ctggtactgg tgatggtggc atcgacgggt ttgccgtcta tccacagggt catatttgat
     2581 actaccgctc ctggtggcag agcaatttcc gcccgagcct cctgagctgt gctgctggcg
     2641 tttttcagta caaaggtcca gctcatgttg gcagtcaggc tgcgggcatt gatcagaccg
     2701 gacagttgtg accgcttgag ggaaaagcct ttgatggcct cccccactac ctgcctggct
     2761 agaaagttat cgtagctgct ggaggtaagc gtatcgtttg actggtcgtc aacggccagg
     2821 tctgtaatgg cttcataagg ttgggatgtg agggcgaaat aaagttgcct gctgcttgag
     2881 tcgttgacac caaggaagag tccggagagt ccgccggtgc gcggcgccgc tatgtctcgc
     2941 tttagctcct gagtactgtc cagtagaggg tttctcaagt tccttagtgc ttctgttctc
     3001 ttgtcggaat cttccgtgag cgccgccttt tcgttgactc ttacggctac ctgcctggct
     3061 tccgaagcac ccactgccag aagagacagc attactcccg ccagactgta gattaactgg
     3121 ctggtgcgag cgccatcggt ttcccagatc aggcatagca tttgcatcaa ccgcaggctt
     3181 gctaccaggg agcagaatgt gagcatggcg caaatggcgt attcgctctg gtgactgatg
     3241 agattgctgt cgatagccgg ataacccagg gcgcaggcgg cgacgcagcc gaccagaaca
     3301 aggccggctg cccctgccga aagaccgtta aggagcccga tgcggagtac attggccagg
     3361 ttgcgctgtt taaggcgctg ccagaccaca taattgccta tcggtatggt caggacaatc
     3421 ccatactg
//
LOCUS       DAOQNZ010000255         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13486_length_3428_cov_3.452416, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000255 DAOQNZ010000000
VERSION     DAOQNZ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13486_length_3428_cov_3.452416"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKW73_05835"
     CDS             complement(<1..809)
                     /locus_tag="PKW73_05835"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF016553.2"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single strand break in
                     duplex DNA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNA72835.1"
                     /translation="MRRFAALFESLDSSNSQEAKLKSLLEYFREGEAEDLAWATYFLS
                     GRKIKRVLSTTHLRSFCLSECGIADWLFQECYDNVGDLAETVALLLPEGDSQVPANSL
                     SAVVKDLQRLKNLNPTELNECLRSYYGQMNTTEKLVFGKLLTGGFRVGVSAKLTAKAI
                     ARLSGLSENIVQERLMGDFKPSAEFYSGLISEEGTAGDAAIPYPFFLAHPLHSIEEGA
                     AALDISSCSLEWKWDGIRSQLIKRGGQVFVWSRGEELITERFPEVALAAARL"
     gene            complement(809..1834)
                     /locus_tag="PKW73_05840"
     CDS             complement(809..1834)
                     /locus_tag="PKW73_05840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HNA72836.1"
                     /translation="MQDLVVVRENGLYCPSGDFYIDPHRPVARALITHGHSDHARAGS
                     QTYIAERSGLSILRHRLGDDAHSRLQGVAYGETLFFGETRVSFHPAGHVLGSAQIRIE
                     HEGHVTVVSGDYKTVFDPTCVPLEVVPCDTFITESTFGLPIYRFPDSEKVAGEILQWW
                     EVCRSNGKIATLFAYSLGKAQRILKTLSLLGAFPAPIYTHPAVEAINELYRQEGVNLP
                     DTRLLTSEHKAQTLRQEGALIIAPPGLAENSFGRRFEGESAFLSGWMQVRGIRRRRAL
                     DKGFIVSDHADFQGLIKTVEGTGARRVLVTHGSAQALVRYLQERGLQAFDLKDLYSHH
                     EQYAGEE"
     gene            2431..>3428
                     /locus_tag="PKW73_05845"
     CDS             2431..>3428
                     /locus_tag="PKW73_05845"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNA72837.1"
                     /translation="MNTGVIEKRAVLSKPGYPVPSKKLVDWVEEVATLCQPERVHWCD
                     GSQEEYDRLCNEMVENGMLLRLNPELRPNSFLARSHPSDVARVEESTFICTTHEVDAG
                     PTNNWMPPTRAKEILRGLFDGCMRGRTMYVIPFCMGPLGSEIAQIGVEITDSPYVVAS
                     MRIMARMGKAVVDLLGSEGKFVPCLHTVGKPLAPGETDNEWPCNKETKYICHFPETRE
                     IWSYGSGYGGNALLGKKCFALRIASSMAREEGWLAEHMMILGLESPRGQKVYVAAAFP
                     SACGKTNLAMLEPTAEFADWKLTTIGDDIAWLKPGKDGRLYAINPEAGYFGVAPGTS"
BASE COUNT          884 a          964 c          892 g          688 t
ORIGIN      
        1 agtctcgccg ccgccaaggc cacctccgga aagcgctcgg tgataagctc ctcgcccctg
       61 gaccagacaa aaacctgccc gccccgtttg ataagctgcg accttatacc atcccacttc
      121 cactccaggc tgcaggaact gatatcgagg gctgccgccc cctcttctat ggaatggagc
      181 ggatgggcca gaaaaaacgg gtagggaata gccgcatcgc cggcagtacc ttcctcactg
      241 atgagaccgc tatagaattc ggcgcttggc ttgaagtcgc ccatcaggcg ctcttgaacg
      301 atgttttcgg atagaccgct caacctggca atggccttcg ccgtaagctt agcggaaaca
      361 ccgacacgaa atccaccggt gagaagtttg ccgaagacca gcttctcggt agtattcatc
      421 tgaccgtagt aagagcgcag gcactcgttc aattcggtag ggttcaaatt cttgagacgt
      481 tgcagatcct taaccacagc cgataagcta tttgcaggca cctgactgtc tccctcaggc
      541 aagagcaagg ccacagtctc agccagatct ccgacgttgt cataacattc ctgaaaaagc
      601 cagtccgcaa ttccacactc ggaaaggcaa aagctgcgta agtgcgtggt ggagagcaca
      661 cgcttgatct tccgcccgct caagaagtaa gttgcccagg ccaggtcttc cgcctctccc
      721 tcgcgaaagt actcaagcag actcttcaac ttagcttcct ggctgttgga agaatccaga
      781 ctctcaaaca aagcagcgaa gcggcgcatt actcttcccc tgcatactgt tcgtgatgac
      841 tataaagatc tttgagatcg aaggcttgca agcctctctc ctgcaagtag cgcaccagtg
      901 cctgggcact gccatgggta acaagaacac ggcgagcacc ggtgccttcc acggttttga
      961 tgagaccctg aaagtcggca tgatcggaga cgataaagcc tttatcaaga gctcgacgcc
     1021 tgcgtatgcc cctcacctgc atccatcctg aaaggaaggc gctttcccct tcaaacctgc
     1081 gcccgaaact attctccgcc aggcccggcg gggcgataat gagagcccct tcctgcctca
     1141 atgtctgggc tttatgctct gaggtcaaca atcgcgtatc aggaagattc acgccttcct
     1201 gccgataaag ttcattgatc gcttccacgg ccggatgagt gtagatggga gcaggaaacg
     1261 cacccaagag gcttagagtt ttaagaatgc gctgggcttt accgagactg taggcaaaca
     1321 gggtggcgat ttttccatta ctacggcaga cttcccacca ctgcaaaatc tcgccggcca
     1381 ccttctcgct gtcaggaaac cggtaaatcg gcagaccgaa ggtagactca gtgataaagg
     1441 tatcgcaagg caccacctca agcggcacac aggtagggtc gaagacggtc ttgtaatcac
     1501 cggacaccac ggtcacatga ccctcatgct cgatgcgaat ttgcgcagag ccgagcacat
     1561 gaccggccgg atggaaagaa accctggtct ctccgaagaa aagtgtttca ccataagcca
     1621 ccccctgcag gcgcgagtgc gcgtcgtcac ccaggcggtg acgcaaaata gagagcccgc
     1681 tgcgctcggc tatataggtc tgagaaccgg cgcgagcatg gtcggagtgg ccatgggtaa
     1741 tgagagccct ggcaaccggc cgatggggat ctatatagaa gtcaccggag gggcaataaa
     1801 gcccgttttc tcgaacaacc accagatctt gcacaatttc tacctgggcg cttaaccctt
     1861 taattgtggc aaaaacaaag aagattgcac cttagaattt gacaaaaact tagcagccgg
     1921 ctccgtcaag gcgcagacaa agtgattcca ccgacggctt cccgatgcga gagatccatc
     1981 catcagaacc caaaccaact ttgccaaagt ctgttcaagc acctgaactc tccgaaaacc
     2041 cgcctaccga cgacatctac accgggttgc attgccgaca ttgcttcaca gaccgcaaac
     2101 tttcagcctc tttgtgaaga cgttcaaagt tatgcaaacg aacgtgacag tcagacgccc
     2161 ttttgtgaag acaggcaaac ttttgcaacc cggaacgcaa caaatgcaca agcttgtatt
     2221 acgtgttcaa attagctcca gcgcccgcat tggagacacg agccggaaga tggctgatgc
     2281 cactgcgtct tgaggatgag gacaatcatc cttctggagc ctaatataga cgtcactgca
     2341 acagtgatgg caccggtggt gcaactcaaa tagagaaaaa gacgaaaaga gatgaccaga
     2401 gcgtgccggt tggaaattca ggagtcaaat atgaatacgg gagtaataga aaagcgggca
     2461 gtgctgagca agcccggcta cccagtgcca tctaaaaaat tggtcgactg ggtggaagaa
     2521 gtggctacac tttgtcagcc cgagcgagtt cactggtgcg acggctcgca ggaagaatac
     2581 gacaggttat gcaatgagat ggtagaaaac gggatgctgc tgcgcctcaa cccggaattg
     2641 cgccccaata gctttctggc gcgttcgcat ccatcggacg tagcccgcgt agaggaaagc
     2701 acctttatat gtactacaca tgaggtagac gccggaccga cgaataactg gatgccgccg
     2761 accagggcca aggaaattct gcggggtctc tttgacggct gtatgcgcgg tcgtactatg
     2821 tatgtgattc ctttctgtat gggccccctg ggctcggaaa tcgcgcaaat aggagtggaa
     2881 ataaccgatt ccccttatgt cgttgccagc atgcgcatca tggctcgcat gggcaaagcc
     2941 gtagtagatt tactgggcag cgaaggcaaa ttcgtgccct gcctgcacac ggtgggcaag
     3001 ccactcgccc ccggagagac cgacaatgaa tggccctgca ataaggagac caaatacata
     3061 tgtcacttcc cggagacacg cgaaatttgg tcttacggct cgggatacgg cggcaacgcc
     3121 ctgctcggca agaaatgctt tgccctgaga atcgcctcca gcatggcccg agaagaaggc
     3181 tggttggccg agcacatgat gattctcggt ttggaatcac cccggggtca aaaggtctat
     3241 gtagcagcag ccttccccag tgcctgcggc aagaccaacc tggccatgct cgaaccgaca
     3301 gccgaatttg ccgactggaa actgaccacc atcggtgatg acattgcctg gctgaaaccc
     3361 ggcaaagacg gacgcctcta tgccatcaat ccggaagccg gatacttcgg cgtagcgccc
     3421 ggcacatc
//
LOCUS       DAOQNZ010000256         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13554_length_3419_cov_2.745244, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000256 DAOQNZ010000000
VERSION     DAOQNZ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13554_length_3419_cov_2.745244"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..753)
                     /locus_tag="PKW73_05850"
     CDS             complement(268..753)
                     /locus_tag="PKW73_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72838.1"
                     /translation="MENGVDKFNLSEVLQQVGNAAYSISCHIIFDLAVHGLAIALFML
                     TIGLILASMRHRLSGPFLVVGRKLGLVCSIGAVPGVVTLISSHSLPPVGVYNINSLGY
                     ICLWSLITAHMLGEETNYQWTVKKDGPVKPAEQGSGDPAEAETSLAAQPSEGENVPVG
                     R"
     gene            complement(850..2337)
                     /locus_tag="PKW73_05855"
     CDS             complement(850..2337)
                     /locus_tag="PKW73_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72839.1"
                     /translation="MAFTKQYASLDGNRRGRGDGGIHSYLPRRGACLSLSNVFLKVKR
                     PSLATLSVLALSFLLPLSAAAQNDSSGFALTTPISDQPARSTSDLNLETVSSHDLFIS
                     ASADAAAGRLESAVLKYKEVVRREPTNVSARNNLAVCLKLQGNKEAALQEFEKTLAQS
                     PNQPELYNNLATSQIALGKYDQALDSLYKALRLKPDFSDAHRNLAHVLSLKGDNEGAV
                     TAYQEAIQEAGAKWQKAPPSDQVNGEGEKSAEPDADVKLPLDAAARAQLGDLHYELGD
                     CLRLVHRFEQALLEYKTTLKLCSHSSDDFKESVKLKCAKCYEGMGRYEEARQILNELL
                     EKSPDDTDALNCLGVVLWQMKQLPESVYVLERALKLEPAYPQARNNLGIALYELKRYD
                     EAVNVWRQALSLKPDYPEAHYNMGVALYQSGLYDQAVEAYKECLTYAPNDANAHNNLG
                     LALLRLGNKAEALAQWRKAIECDANCAEAYTNLGKLLKETAVQPQ"
     gene            2381..2818
                     /locus_tag="PKW73_05860"
     CDS             2381..2818
                     /locus_tag="PKW73_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72840.1"
                     /translation="MAQITIKVSTLIKVIGTTFAFSFLLGLLTAKMPLAAAQETFLGQ
                     KFTREETMLLNLSHGKGSAGFFLLESERLQKDITHNLERAMSQLEQVEKAYARSKGKP
                     DDKYLASTQLKLVNARQRSEELSEHISNTFRDMKRTVKDVLLR"
     gene            complement(2824..2895)
                     /locus_tag="PKW73_05865"
     tRNA            complement(2824..2895)
                     /locus_tag="PKW73_05865"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2861..2863),aa:Ile,seq:cat)
     gene            complement(2914..>3419)
                     /locus_tag="PKW73_05870"
     CDS             complement(2914..>3419)
                     /locus_tag="PKW73_05870"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014804.2"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNA72841.1"
                     /translation="EMNTRIQVEHPVTEMITGFDLIKEQIRVASGEPLSLRQEDVTFN
                     GHAIECRINAEDPARNFLPSPGRVDAYIAPGGPGVRIDSHCYPGYVIPPYYDSLVSKL
                     IAWGRDRDEAIQRMQRALDEYAVTGIKTTIPFHQEVLAHPVFQAGDVTTDFIEKHMTP
                     ERSKITL"
BASE COUNT          829 a          893 c          893 g          804 t
ORIGIN      
        1 aggggtaagc tgggcgcaga cggctgcccg gtctgccatt gagccggcgc cggcgcggga
       61 ttgaagctga gaagcccggt cagcaaggtg gaggcaagga gccatgaata ttgacgttgt
      121 agggtcatag cattcgcctc catgaggaat tttgccggtg tctttgcagg ctctgtcgcc
      181 gtttttgcaa ctcatctgtg tctgctaccg gctatctaca gtttagctca agaacttagg
      241 aagagctaaa tgttgaaata atgcagccta gcgacccacc ggcacatttt caccttcaga
      301 cggctgggcg gcaagggaag tttccgcctc cgcaggatca ccgctgcctt gttcggcagg
      361 cttgaccgga ccgtccttct tgaccgtcca ctgataattg gtctcttcgc ccagcatatg
      421 tgcggtaatg aggctccaga gacagatgta gccaagcgaa ttgatgttat agacaccaac
      481 ggggggcaaa ctgtgactgc tgatcagggt aactacgccg ggtacggcac cgatggagca
      541 gaccagacca agtttgcgac cgacaaccag aaacggcccg ctcagcctgt gccgcatgga
      601 ggcgaggata agcccgattg taagcatgaa gagggcaata gccaggccgt gcacagccag
      661 atcgaagatg atgtgacagg agatactgta ggcagcgtta ccgacctgct gcaagacttc
      721 actcaagttg aatttatcga caccgttttc catattgagc ctgctctatt tgattcccct
      781 tttcactcca cccaccggca actgacttaa atgccgctca caagtctcac atctcatgat
      841 cacttgtcat tactgaggtt gtaccgcggt ttcctttaga agtttgccta aattcgtata
      901 agcttccgcg caattagcat cgcactcaat ggctttacgc cactgggcaa gcgcctccgc
      961 cttgtttccc aggcgtaaaa gagcaagacc taaattatta tgagcgtttg cgtcattggg
     1021 ggcataggtc aaacattcct tataagcctc cacggcctgg tcgtatagcc ccgattgata
     1081 gagagccacg cccatattgt aatgagcttc gggataatca ggtttgaggc tgagagcctg
     1141 cctccagaca tttaccgctt cgtcatagcg tttcagctca taaagggcta tgcccagatt
     1201 gtttcgcgct tgcggatagg cgggctccag tttgagggct cgctccagca cataaacaga
     1261 ctcgggcaac tgcttcatct gccagagcac cacccccagg cagttaaggg catcggtgtc
     1321 atcgggcgat ttctccaata gttcattgag tatctgcctg gcttcctcat agcgccccat
     1381 accctcatag catttggcac atttgagttt gaccgactct ttaaaatcat ccgatgagtg
     1441 actacaaagt ttgagcgtgg ttttgtattc aagaagcgcc tgttcaaagc ggtgcaccag
     1501 gcgcaggcaa tctcccaatt catagtgcaa atcgccaagc tgggctctgg cagcggcatc
     1561 tagtggaagc tttacatctg catcgggctc cgcagacttt tccccttcac cattaacctg
     1621 gtcggagggc ggtgccttct gccatttggc accggcttcc tgaattgcct cctgataggc
     1681 agtcaccgcc ccctcattgt cgcccttgag ggaaagcaca tgagccagat tgcggtgcgc
     1741 atcggaaaaa tccggcttca gtcgcaaagc cttatagagc gaatcgagag cctgatcata
     1801 tttaccaaga gcaatctgac tggtggccag gttgttgtag agttccggct gattgggact
     1861 ctgggccagg gtcttctcaa attcctgcaa agccgcttcc ttgttgccct gtagcttgag
     1921 acaaacggcg agattattgc gagcggaaac attggttggt tcccgacgca ccacttcttt
     1981 gtacttgagc acggccgact ccaggcgacc ggcggcggca tcggcagagg cactgataaa
     2041 gagatcgtga ctgctcaccg tctctaaatt gaggtcggaa gtggagcggg ccggctgatc
     2101 ggagatgggg gtggtgaggg caaaaccact gctgtcattt tgagcggccg ccgacagcgg
     2161 caagagaaaa gagagggcaa gcacggacaa agtagcaagg gaaggccgtt tcactttcag
     2221 aaatacatta ctgaggctta agcaggctcc tcgcctcggc aaatagctat gaatgccgcc
     2281 gtcgccacga ccgcgtcggt tgccatcaag actcgcatac tgttttgtaa aagccatgac
     2341 gtatatacta ctgcttgagg accagtgtga gggcggaaaa gtggcacaaa ttacgatcaa
     2401 agtatcgact ctgatcaaag ttattggtac gacttttgcc ttttcctttc tattgggtct
     2461 tctgacggca aaaatgcccc tggctgcagc ccaggaaacc ttcctgggac agaaatttac
     2521 cagggaagag acaatgcttc taaacctttc tcacggcaaa ggctctgccg gcttcttctt
     2581 gcttgagtct gagcgtctgc aaaaggatat tactcacaac ctggaaaggg ccatgagcca
     2641 gcttgaacag gtggagaaag cctatgctcg ttccaaaggc aaacccgacg acaaatacct
     2701 ggccagcacc cagttgaaac tggtcaatgc cagacaacgc agcgaagaac tgagcgagca
     2761 catctcgaat actttcagag atatgaaacg cacggtcaaa gatgttcttc taagataaga
     2821 atttggctcc gactggattc gaaccagtaa ccaggcgatt atgagtcgca cgctccacca
     2881 ttgagctacg gagccccaca ggcaagctct attctacaac gtaattttgc ttctttcagg
     2941 ggtcatatgt ttttcgatga agtcggtggt tacatcaccg gcctgaaaaa ccggatgcgc
     3001 cagaacttcc tgatgaaaag gtatggttgt cttaataccc gtcacggcat actcatcaag
     3061 agccctttgc atgcgttgaa tggcttcgtc gcgatcacgc ccccaggcta tcaacttact
     3121 gaccagagag tcgtaataag gcggaattac ataaccggga tagcagtggc tatcgatgcg
     3181 cactccggga ccgccgggag ctatgtaagc gtctacccgt cccggtgaag gcaggaagtt
     3241 gcgagccgga tcttcggcat tgatgcggca ttctatggca tgaccattga aggtgacatc
     3301 ttcctgccgc aaggaaagtg gctcgccgga ggctactcga atctgctctt tgatcaagtc
     3361 aaaacctgta atcatctcgg taacagggtg ttcaacctgg atcctggtgt tcatttcca
//
LOCUS       DAOQNZ010000257         3413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13596_length_3413_cov_3.246575, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000257 DAOQNZ010000000
VERSION     DAOQNZ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13596_length_3413_cov_3.246575"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PKW73_05875"
     CDS             complement(<1..737)
                     /locus_tag="PKW73_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72842.1"
                     /translation="MTIVMGLAAFLAALLCISAAIDGAHVYLVQQELIRVTDSAALAG
                     AARLTGESATVISAVSDVAAANRVDGKPIIDNPPRTTLAVTINNPAGQNQGTVTVATS
                     TTTRHIFMPLLGRHEDHVYAQSTAGAGAPVTALARNKVFPLAVSYDAKPSDDPQARAL
                     KDLQLGDITTLYLSSQQFKNAAFTSLGPESTSASAVKSLIDGHLKQSNTEESVQVGDS
                     IDMGNGVMGLKYLANSPYREILLGKECL"
     gene            complement(832..1263)
                     /locus_tag="PKW73_05880"
     CDS             complement(832..1263)
                     /locus_tag="PKW73_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72843.1"
                     /translation="MMRHHHGTNLAEATAAIVLFLPLFMLMIVVTASVSTACLLQNAL
                     SASAHRAARSLALACLDNPDVENNRSQQEALVFDQIRIPGIIVDSRQFEASFDNQSQT
                     QSVTVTVSYSPGQYGLYSGPNTFALTPLPLNLKASATHGLR"
     gene            complement(1516..2271)
                     /locus_tag="PKW73_05885"
     CDS             complement(1516..2271)
                     /locus_tag="PKW73_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNA72844.1"
                     /translation="MLAPERQKQILQKLESRGQVLVQDLVRELGVSEDTIRRDLKELA
                     SAGLLKKVHGGAIAASTVPYAHAERRRLNREAKAAMALKATQFIQNGMLIFMDGGTTT
                     LEVAPHLKARSLENVTVVTHSPVMAQSLSEIAGLEVFLLGGKLIKDLLIVVGPNSLKE
                     ASRFRPDLSIVSAHGMSTAAGATVESGEDAAVKRQFIENSAETLVLAGHEKIGFVASF
                     QIAALDEISYLITDEVKDLSLFNHGNLTVLACE"
     gene            2404..2946
                     /locus_tag="PKW73_05890"
     CDS             2404..2946
                     /locus_tag="PKW73_05890"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72845.1"
                     /translation="MARIAVDMDEVLADALGEHLSRYNNLFGANLSREDMIGRALRHA
                     VPLEHAHHAEALVHNEDFFADLAIFPHAQEVLARLSENHEIYIATAAMEVPLSFRSKY
                     DWLLRHFPFISPMNFVFCGDKGIIDADYLIDDNARHFSRFKGTGLLFDAPHNQHVRGF
                     KRVHNWLEVEEFFVREAALV"
     gene            complement(3088..>3413)
                     /locus_tag="PKW73_05895"
     CDS             complement(3088..>3413)
                     /locus_tag="PKW73_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNA72846.1"
                     /translation="ADIQPGDIIQKIDGKAVLSPRDVQTTVRAHRVKDKLNFLLLRDG
                     AIKTAEVTIGAYPDYIDLKERSEGLNKSTPQTSPGQTADDNKNPPKATIPQGKPQVKK
                     PAPFR"
BASE COUNT          649 a          868 c          974 g          922 t
ORIGIN      
        1 aggcattcct tgcccaggag tatctcccgg tatgggctat tggccaggta cttcagaccc
       61 atgacgccgt tacccatgtc gatgctgtcg cccacttgca ccgattcttc ggtattgctt
      121 tgtttgagat gtccgtctat caatgacttt accgccgagg cactggttga ctcgggaccg
      181 agggaagtga aagcggcgtt cttaaattgc tgcgagctca gatagagagt agtgatatca
      241 cccagttgca ggtctttgag ggctctggct tgcgggtcgt cggatggttt ggcatcataa
      301 ctaaccgcca gcggaaagac tttgtttcta gccagagctg tgacgggtgc tcccgctcca
      361 gcagtgctct gggcgtaaac atgatcctca tgccgcccca gtaggggcat gaaaatatgc
      421 ctggtagtag tggatgtggc tacggttacc gttccctgat tttgtccggc gggattattt
      481 atggttaccg ccagtgttgt acgcggcgga ttgtcaatga ttggtttgcc gtctacccga
      541 ttggctgcgg ctacgtcgct tacggcgcta atgacggtgg cgctctcgcc tgtgagacga
      601 gcggcgccgg ccagggcggc gctgtctgtc acgcgtatca gttcttgctg aaccaggtag
      661 acatgggcgc catcaattgc cgccgatatg cagaggaggg ctgctagaaa agctgccagc
      721 cccataacaa tagtcatctg gcctcgctgg ttttgcacag ggcgcttttt catctttgtc
      781 tggttctgca tcatcttttt tcctgctgtg ataaggcatt gggtcaggtt cttatctcag
      841 tccgtgagtg gcactggctt ttaagttcag aggcaacggc gtcagggcaa aggtattcgg
      901 tccgctgtaa agtccatatt gaccggggct gtaggaaacc gttaccgtca ctgactgcgt
      961 ctgtgactgg ttgtcgaagc tggcttcaaa ttgcctggag tcgacgataa taccggggat
     1021 gcgaatttga tcgaagacca gcgcctcttg ctggctgcga ttgttctcta cgtcggggtt
     1081 gtcgaggcaa gccagggcca gggagcgggc tgctctatgg gccgaggctg agagcgcatt
     1141 ttgcagcaag caggccgtac tgaccgaggc cgtcaccacg atcatgagca tgaagagcgg
     1201 caaaaagagc acaattgccg ccgtagcctc ggccagattt gtaccgtggt gatgtctcat
     1261 catttacgca aatgcccgcc agctctgccc gtattgtcaa tgaccgggat tgccaattag
     1321 ccagtttgtt ttcctgaacc ctgaaggtgc cttgttcgct ttaggaagca tgaaaatctc
     1381 aagtgcttca gttctatcat tgattagttc ccagcttgct gcgtcgcccc gggggctgag
     1441 ttatccgcta atcataaagt ttccagcctg tcccctgagt gggggctgct tcctgggttt
     1501 ccgcgtgccg acgctctatt cacaggctag cacagttaaa ttgccgtggt tgaaaaggct
     1561 gagatctttg acttcatctg taatcaaata ggatatttca tccagagctg caatttgaaa
     1621 ggaggctaca aagcctattt tttcgtgtcc ggccagcacc agagtctcgg cgctattttc
     1681 gatgaattgt cttttgacgg cggcgtcttc cccgctttct accgtggctc cggctgcggt
     1741 actcattccg tgtgcgctga cgattgatag gtcggggcga aaacggctgg cctctttcag
     1801 ggagtttgga ccgaccacga tcagtagatc ttttattagt ttgccgccca gtaggaagac
     1861 ctcaagaccg gctatttcac taaggctctg ggccatgacc gggctgtggg ttacaaccgt
     1921 gacgttttcc aggctgcggg cttttaaatg cggggccact tccagagtgg tggtgccgcc
     1981 atccatgaat atgagcatgc cgttttgaat gaactgagta gccttgaggg ccatggctgc
     2041 ttttgcttct ctattgaggc ggcgtctttc ggcatgggca taaggcacgg tggaggcggc
     2101 aattgcaccg ccgtgtacct tcttcaataa gcccgccgaa gccagttcct tcaggtccct
     2161 cctgatcgta tcttccgata cccccaattc ccgcaccaga tcctgtacca gcacctggcc
     2221 acggctctct agtttttgca ggatttgctt ctgtctttcc ggggccagca ttgtcttcgc
     2281 cttttattcc agtcggcagt cggggatgga ggtgcaggca cttgacctgc gggaatattc
     2341 ggtattatac tggattgtgc tggaattggc aggtatttgc aggaatctgc ccgaaggagg
     2401 cccatggcta gaatcgcagt ggatatggac gaagtgctgg ctgatgctct gggcgagcat
     2461 ctcagccgtt acaacaacct gtttggcgcc aatctcagtc gggaagacat gattggtcgg
     2521 gctctcaggc atgccgtgcc cctggagcac gctcaccacg ccgaagcgct ggtgcacaat
     2581 gaggacttct ttgccgacct ggctattttt ccccatgccc aggaagtact agcgcgattg
     2641 agcgagaacc atgaaattta tatcgccacc gctgccatgg aagtgcctct atcgttccgc
     2701 tccaagtatg actggctact gcgtcacttc ccctttatct ctcccatgaa ctttgttttt
     2761 tgcggagaca agggcataat cgatgccgac tatctgatcg acgacaatgc caggcacttc
     2821 agccggttca agggcacggg actgctcttc gatgcacctc ataatcagca tgtgcgaggc
     2881 ttcaagcgcg tgcataactg gctggaagtg gaagagttct ttgtgcgaga agcagctcta
     2941 gtttaggttt aggtttggat tcagcttgag gatcaggatc aggtttcagt tctgatcctg
     3001 attccgcttc cggaacctgt tagtttctgc ttcggtgaaa gcggcgtctt gctctacatc
     3061 tgcgcgagct tggtggcggc tagtgcttca acggaaaggg gctggtttct tcacctgcgg
     3121 tttgccctgg gggatggtgg cttttggcgg gtttttgtta tcgtcggcgg tctgacctgg
     3181 actggtctgg ggtgtggatt tgttcagtcc ctccgaccgt tcctttagat cgatatagtc
     3241 gggataggct ccaatcgtta cttccgccgt cttgattgct ccatcgcgca gaagcaggaa
     3301 gttgagcttg tctttgacgc ggtgtgctct gaccgtggtt tgcacgtctc taggcgaaag
     3361 cacggctttg ccgtcgatct tttgaatgat gtcgccgggc tggatgtctg cgt
//
LOCUS       DAOQNZ010000258         3407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13640_length_3407_cov_3.203461, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000258 DAOQNZ010000000
VERSION     DAOQNZ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13640_length_3407_cov_3.203461"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKW73_05900"
     CDS             <1..224
                     /locus_tag="PKW73_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72847.1"
                     /translation="SIAGGLASIVDLPVTEVSYDNDASNVYVTVTTHVTVKPFLTIPF
                     FTVIPGLGAPATYTISSSRMLENPRFLAY"
     gene            239..676
                     /locus_tag="PKW73_05905"
     CDS             239..676
                     /locus_tag="PKW73_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72848.1"
                     /translation="MIKCNFNKRKQRKAGGRDKSGQTLIEALTGFIVIIPLGLMAVNL
                     VAVVSTSQSNEQWAELAARAAAAQANEQNARKAAENSLARAKLTSIIKSVTVSKVDYD
                     LARGQVTIGTTMQVAMPVPMPFLSELECHADSIQPIVATPAPI"
     gene            complement(691..927)
                     /locus_tag="PKW73_05910"
     CDS             complement(691..927)
                     /locus_tag="PKW73_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72849.1"
                     /translation="MEKTEDAGDEQEGTDYNETEGGTPRLSRPFLLLSLVLLLSLVLL
                     HPLSLLHPLSLLHPLSLLHLLVLLVPLVRHVLFN"
     gene            940..2394
                     /locus_tag="PKW73_05915"
     CDS             940..2394
                     /locus_tag="PKW73_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72850.1"
                     /translation="MGGSRQVRNAVDAGVLNISKRIVEVKIPVTDKFKDVGDSTGSVS
                     LSNVNRIWGKAYLINANAEAMKADGQANSDTQAGADGAYALARTLNDDLFNQVSSDNV
                     LNMYFQQLAHQREARMLGEGTKVERSKVETCSIAMVDRGAESNLSFSGGQMPGSVSPK
                     SRNFGGKSFFSGYVPMKANEKDFCFTSFRQGEMPHLIDDAYFESNSAVKPTGSSYSPL
                     PNAFKRYGEVEAPAGKLTAVACAVANPQRSYNVAIPYSFVTIQVGNTAKWYVEGKKIK
                     ETTYGFKTEEQKEIKDFPLSTGGKLYGNASLGNEYSAASSLLDVINAVPGNHNSAFKK
                     LVQRVQEIDPAFSMEKLMKLLESQVFTKTSADGSLPPSKYFIYPTYSSPDKTDPTIKI
                     GSSKGDLPSWLNPDNPPDGMEKLVMQEDKMRDKPNFCWCYVVGGSSPTVKHYTDVYGN
                     IMWQPGTGYGQHLGELRFARLTEIYFGEQNQAAP"
     gene            2397..3338
                     /locus_tag="PKW73_05920"
     CDS             2397..3338
                     /locus_tag="PKW73_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72851.1"
                     /translation="MKKGLSSRKLGDNQINGDQDKNTVKRAKTQWQQKDAPAKVRWFS
                     LALALTSCLCGQTAAVALSPDLKEGIAAYNTGDYKEAVSHLSGALSQDFNNAVLHYYL
                     ANSFVHLGQRDAAIREFRIAYALEPEKEAGKLSKLALKYMGVETGAGEAEQKPLPPPL
                     PPPKSQTELRKEKTAASLKEQVDRERQLNERIAAELADSTARRGADLVSRQKNELLDA
                     MKYIRRGRVHQLPLPADSLKQLEAIKSFYDGQSSMHKLSAGKRTSELQKSAENLQDLM
                     SEKNCKKGPKLIPEGSNLYVRSYHNSTDASKESTSHK"
BASE COUNT          940 a          975 c          858 g          634 t
ORIGIN      
        1 gctccatcgc cggcggtctg gccagcattg tcgacctgcc ggtcacggaa gtcagctacg
       61 acaacgacgc cagtaatgta tatgtcaccg tcaccaccca tgtgacggtc aagccctttt
      121 tgaccattcc ctttttcacc gtcatccccg ggctgggggc acctgccaca tataccatca
      181 gcagttccag aatgctggaa aaccctcgct tcttagctta ctagccgcag atgtcgccat
      241 gatcaagtgt aatttcaaca aacgaaaaca gagaaaagcc gggggaaggg acaagagcgg
      301 tcagactctc atcgaagctc tcaccggctt catcgtcatt attcctctgg gactgatggc
      361 cgtgaacctg gtagcagtag tgtcgacctc acaatcaaac gaacagtggg ccgaacttgc
      421 cgcccgagcg gcagccgctc aggcaaacga acagaatgcc aggaaggcag ccgagaattc
      481 cctggccagg gccaaattaa cttccatcat caagagtgtc acagtcagca aggtcgacta
      541 cgatctagcc cgggggcaag tcaccatcgg taccaccatg caagtagcga tgccggtgcc
      601 gatgcccttt ctttccgagc tggaatgcca cgccgattcc attcaaccca tagtcgccac
      661 ccctgctccc atatgaacaa ttttccacat tcaattaaac agaacatgcc gcaccagggg
      721 aaccagcagc accagcagat gcagcagcga cagcggatgc agcagcgaca gcggatgcag
      781 cagcgacagc ggatgcagca gcaccagcga aagcagcaaa accagcgaaa gcagcaaaaa
      841 gggtctagac aggcgcgggg tgccgccctc ggtctcgtta tagtcagtgc cttcctgctc
      901 gtcgcctgca tcttcggtct tttccaactg agcatgctca tgggcggctc cagacaggtg
      961 cgcaatgccg tagatgccgg ggtgctcaat atatccaagc gtatcgtcga agtaaaaata
     1021 ccggtcacgg ataaattcaa agacgttggt gactctaccg gtagtgtcag cctcagcaat
     1081 gtcaatcgca tctggggcaa agcctatctc atcaatgcca atgctgaagc catgaaagcc
     1141 gacgggcagg caaactccga cacccaggcc ggggccgacg gcgcctacgc gctggctcgc
     1201 actctcaacg acgatctctt caatcaggtc tcaagcgaca atgtgctcaa tatgtatttt
     1261 cagcaactgg cccatcaaag agaagctcgc atgctgggcg aaggcacaaa agtagagcgc
     1321 tcgaaagtag aaacttgctc catcgccatg gtagaccggg gcgccgaaag caatctatcc
     1381 ttcagcggcg ggcaaatgcc cggcagtgtc tcgccaaaat ccagaaactt cggcggcaaa
     1441 tcattcttca gcggctatgt tccgatgaaa gccaacgaaa aagatttctg cttcacaagc
     1501 ttccgtcagg gagaaatgcc ccacctgatt gacgatgctt atttcgagag caatagcgcc
     1561 gtcaagccta ccggttcgag ctattcaccc ttgcccaatg ccttcaagcg ctacggagaa
     1621 gtggaggcac cggcgggcaa actgacggca gtggcctgtg ccgtagccaa tccgcaacgg
     1681 tcatacaatg tggccattcc ctactctttc gtcaccatcc aggtaggcaa taccgccaaa
     1741 tggtacgtgg aaggaaagaa aatcaaagaa accacctacg gcttcaaaac cgaagagcag
     1801 aaagagatca aagactttcc tctctccaca ggcggcaagc tttacggcaa cgccagcctg
     1861 ggcaatgaat acagcgccgc ctccagcctt ctcgatgtaa tcaacgccgt gcccggcaat
     1921 cacaatagcg ccttcaagaa actagtgcaa agggtgcaag agatagatcc cgccttttcc
     1981 atggaaaagc tcatgaaact gctggaaagt caggtcttta ccaaaaccag cgccgatggc
     2041 tccctgcccc ccagtaagta ttttatttat cccacttaca gttcaccgga caaaaccgac
     2101 cccaccatca agataggctc atccaaaggc gacctgccat cctggctcaa tccagacaat
     2161 ccaccggacg gcatggaaaa gctggtgatg caggaagaca aaatgagaga caagcccaac
     2221 ttttgctggt gctatgtggt gggcggcagt tcacccacgg tgaaacacta taccgatgtc
     2281 tacggcaata tcatgtggca gccgggcaca ggctacgggc agcatctggg cgaattgcgc
     2341 ttcgccagac tgactgagat ctactttggg gaacagaacc aggcggcccc ttaaatgtga
     2401 aaaaaggcct gagttcaagg aaattagggg ataatcaaat taatggtgat caggacaaaa
     2461 acacggtgaa gagagctaag actcaatggc agcaaaagga tgcgcccgct aaggtccgct
     2521 ggttctcctt ggctctggct cttacctctt gcctgtgcgg tcagactgcg gcagtggcct
     2581 taagccccga cctgaaagaa ggcattgccg cctacaacac aggcgactac aaagaggcag
     2641 tcagccattt gagcggtgct ctctcccaag acttcaacaa tgcggtgctg cactattatc
     2701 tggctaattc ctttgtgcac cttgggcaaa gagatgccgc cataagagag tttcgcattg
     2761 cctacgccct cgaacccgag aaggaagccg gcaagctctc caagctcgcg ctcaagtaca
     2821 tgggagtaga aacaggcgcc ggcgaagcag agcaaaagcc gctgccgccg cctctaccgc
     2881 cgcctaaatc tcagaccgag ctgcgcaaag agaagactgc cgccagcctg aaagagcaag
     2941 ttgatagaga gcggcaatta aatgagcgca tcgccgccga actggcagat agcaccgccc
     3001 gccgcggagc cgacttggtc tcccggcaga aaaacgaact cctggacgca atgaagtaca
     3061 tcagacgcgg gcgggtgcat caattacctt tgcccgccga cagcctcaaa cagctggaag
     3121 ccattaagtc gttttatgac ggtcagtctt ccatgcacaa gttaagcgca ggcaaaagaa
     3181 ccagtgaact gcaaaaatcg gcagaaaatt tgcaagacct gatgagtgaa aagaactgca
     3241 agaaaggtcc gaagcttata ccggaaggct ccaatctcta cgtgcgcagc tatcacaaca
     3301 gcaccgatgc aagcaaggaa agcacaagcc acaaatagtg ccgcgcccct taactgaaca
     3361 ggtctatatt actggtgtcg cccatgagca aaaatgtgat tatgcca
//
LOCUS       DAOQNZ010000259         3407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13641_length_3407_cov_3.145585, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000259 DAOQNZ010000000
VERSION     DAOQNZ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13641_length_3407_cov_3.145585"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PKW73_05925"
     CDS             complement(<1..1111)
                     /locus_tag="PKW73_05925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNA72852.1"
                     /translation="MTESQPPEAFKPNKVVISLVLAFALVAQLLTFFLLYRALVNIHW
                     YFTGPRLDLTDFSQFYQAGQLIISPHSHRVYDPDVQEWWCLGLIYPQIPIKGLFYNQS
                     VPFLYLFCIPYGLLPYNLAYVVWCLTTGLMALSSLFLLLKESLKGLKTGLFIPLFLVL
                     AWSSIPAYLTVWHGQTTFLLVTCMSLSMCAFLRGRDILSGVFLSLSTVKPQYILLPLA
                     TMFGLRKWRLLAALFATEAALFLLAGCFMGFDTILNYPRVLVSAESSTRFVGVNPHVM
                     ASMRGLLSVLLKHKTAMLITTLTLFASLIPVAWLAFKTPPSNLPESLSPNRSVTHRFL
                     LAFIFTLSLVLSPHSHYFDCLLLTVPAALTLRGMLL"
     gene            complement(1292..2140)
                     /locus_tag="PKW73_05930"
     CDS             complement(1292..2140)
                     /locus_tag="PKW73_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNA72853.1"
                     /translation="MGLEIIIKDTHRGLWYEDGVLTKTLEAGRYKLPKHFDFLGLRNK
                     KVEVTLVDMRERELTIKGQEILTADKVAIRVSIVVQFRVMDPRAATHEVEKYEDRLYT
                     DVQLAARRSLAAMNLEEILTNRNRLSEDILHDVKEQATKYGVNILRADVKDLIFPGNL
                     QEIMNKVLAAERMAEAALIEARTKAEQQKIESETKAHNEKMMSKTLAESKRTMAESEA
                     EVKRIRTTADTESMRLQAQAAAAYADHPALLRLRELETMAELGKNANARLYVGFEKNS
                     RSAGEE"
     gene            complement(2312..3097)
                     /locus_tag="PKW73_05935"
     CDS             complement(2312..3097)
                     /locus_tag="PKW73_05935"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA72854.1"
                     /translation="MATKSVRILRHNKRAMLPLSDDNSDRRLTPWVNYTLIGINIFVF
                     VFLQGMGSNEVFTNSFAAVPQEILSGQDIAGQLPLAGPHGSTTVIQLGETPIPVYLTL
                     FTSMFMHGGFAHIAGNMLYLNIFGDNLEDSMGHGKYLIFYLFCGVVAGLSHVFCTVAM
                     GGNALIPSLGASGAISGVLGGYMVLFPHKSITVLFLRQVMRVPAFACLGMWILFQLAD
                     SSGVLGGSPDAAGVAYAAHIGGFIAGLALVRLFAGEKRNQSYV"
BASE COUNT          805 a          805 c         1012 g          785 t
ORIGIN      
        1 caagcagcat acctctgagg gtgagtgcag ctggaaccgt cagaagcagg cagtcgaagt
       61 agtgagaatg aggactgagt accagggaga gagtaaaaat gaaggctaga agaaagcgat
      121 gggtgacgct tctgtttggg ctcaatgatt caggaagatt gcttggtggt gttttgaagg
      181 caagccaggc taccggaatg agcgaagcaa agagagtgag ggtggtgata agcatggctg
      241 ttttgtgttt caggagcaca ctcaagagcc ctcgcatgct ggccatgaca tgggggttga
      301 ctcccacgaa acgggtagaa ctctcagcgc tgaccagcac ccggggataa ttcaaaatgg
      361 tgtcgaagcc catgaagcag cctgccagaa ggaagagcgc cgcttcggtg gcaaagagag
      421 cggcaagcaa gcgccatttc ctgagaccga acatggtagc cagtggcaat aagatgtact
      481 ggggtttgac tgtggaaagg gaaagaaaga cgcctgagag gatatccctg ccccttagaa
      541 aggcgcacat cgaaagactc atacaggtaa ccagaagaaa agtagtctga ccatgccaga
      601 ccgtcaggta ggctggaatc gaggaccagg ccaggaccag aaagagtgga ataaaaagcc
      661 cggttttgag gcctttcaag ctttctttca gaagtagaaa gagtgaagaa agggccatca
      721 agccggtggt cagacaccag accacatagg ccaggttgta aggcaagagc ccgtagggaa
      781 tgcaaaagag gtagaggaag ggcacggact gattgtagaa cagccccttg attggaatct
      841 gcgggtaaat gaggcccagg caccaccact cctgcacgtc gggatcgtag acccggtgcg
      901 agtgggggct aatgatcagt tggccggcct gataaaactg agaaaaatca gtcaggtcga
      961 ggcgagggcc ggtgaagtac cagtgaatat ttaccagcgc tctgtataaa aggaagaaag
     1021 tgagaagctg ggcaaccagg gcgaaggcca gaaccaaact gatgactacc ttgtttggtt
     1081 tgaatgcttc tggcggttgg ctttctgtca tgggttggct gctttcttct gcggctccac
     1141 gggcatgccg atgacggctg tttcagtgat tatagaggta gggctgaagc ttagccaatg
     1201 gtaaggaagc aaagcgggag cggctccgct tctcaggacc gtccggctga agctttgctt
     1261 gtcaggagat cgggcgggca gcgtgtctgg gttattcttc gccggcgctc cgggaatttt
     1321 tctcaaagcc gacgtaaagc ctggcgttag cgtttttgcc aagttcggcc atggtctcca
     1381 gttcccgcag tctcaaaagg gccggatgat cggcataggc ggcagcggcc tgcgcctgca
     1441 agcgcattga ttcggtatcg gcagtggtgc gtatgcgctt tacttccgct tccgactcgg
     1501 ccatggtgcg tttggattcg gccagggttt tgctcatcat cttttcgttg tgagctttgg
     1561 tctccgactc tattttttgt tgctcggcct tggttcttgc ttcaatcagg gctgcttccg
     1621 ccattctttc tgccgccaga actttgttca tgatttcttg caagttgcct gggaagataa
     1681 ggtcttttac atcggctctc aagatattta caccgtattt ggtggcttgc tccttgacgt
     1741 cgtgaagaat atcttcgctg aggcgattgc ggttggtgag aatctcttcc aggttcatgg
     1801 ccgccagtga gcgtcttgct gccagttgaa cgtctgtgta gagccggtct tcgtactttt
     1861 ccacttcgtg ggtagcggca cggggatcca tgacgcgaaa ttgcacgaca atgctgacgc
     1921 ggatggccac tttgtcggcg gtaagtattt cctgcccctt gatggtcagt tcgcgttcgc
     1981 gcatatccac cagggtgacc tccactttct tgtttcgcaa acccagaaag tcgaagtgtt
     2041 tgggcagctt gtaccttcct gcttccaggg ttttggtcaa gacaccgtct tcgtaccaca
     2101 agcctcggtg ggtatctttg ataataattt caagacccat gtgattctcc gcaattattg
     2161 aggcggcccc gcagcaaagc tgatttggaa gatgtcaaaa cagatgagat agcttgatcg
     2221 cttgcacaaa gtgtttggtt atgtacctca tgtctcgatg gttcctaacc ctgccgggac
     2281 ctatcgagac cttaccactg cgcggaacgg cctaaacata tgactggttg cgcttttcgc
     2341 cggcgaagag tcgaaccagg gccagaccgg cgataaaacc gcctatatgg gcggcatagg
     2401 ctacaccggc ggcatccggg gagccgccta aaacgccgga actgtcagcc agttggaaga
     2461 gtatccacat tcccagacag gcaaaggccg gtactcgcat gacttgcctc agaaaaagta
     2521 cggtgatgct cttatgggga aagagcacca tgtaaccgcc gagtaccccg gaaatggctc
     2581 cggatgctcc cagtgaggga atgagcgcat tgccgcccat ggctaccgtg caaaacacgt
     2641 gagaaagccc ggccacaacg ccgcagaaga gataaaagat gaggtacttg ccgtgaccca
     2701 tggagtcttc caggttgtct ccaaaaatat tgaggtagag catattacct gcgatgtgag
     2761 caaagccgcc atgcatgaac atagacgtga agagagtcag gtaaaccgga atgggtgttt
     2821 cgcccagttg gatgacggtg gtgctaccgt ggggaccggc caggggcagt tgcccggcta
     2881 tatcttgtcc gctcaatatt tcctgcggaa cagcagcgaa ggaattggtg aaaacttcgt
     2941 tggagcccat gccttgcagg aagacaaaaa caaaaatatt gatgccgatg agagtgtagt
     3001 tgacccaggg ggtgaggcgt ctgtcggagt tgtcatcgga taaaggaagc atggctcttt
     3061 tgttgtggcg caggatgcgg acggacttcg ttgccaatac tttagcagtt aatcggcacc
     3121 ttggctgccg caccgtccgc tggaagcgcc cggtcgagcg gtgtgccgcg gttgtggttg
     3181 tcgagggcaa gggctagaat agtcctgagg caatcctttt tgaatggtag tgtttaaccc
     3241 tggcggatcg gaaatcgagc aatgtttgcc gacaatggaa gaatgtccct gataattctt
     3301 tccctgttca cgtcagtgtt catgccggtg gtgtgtgcct ggcattctca ttcctgggct
     3361 cgccctgaaa gtggcgctgc ctccaagctg atcccggccc tcaagct
//
LOCUS       DAOQNZ010000260         3406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13643_length_3406_cov_3.457177, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000260 DAOQNZ010000000
VERSION     DAOQNZ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13643_length_3406_cov_3.457177"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKW73_05940"
     CDS             <1..319
                     /locus_tag="PKW73_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72855.1"
                     /translation="RAGIYRQYFIKWLAATNLIHVALLILILSRLDIALFLRPEYMLQ
                     IAAAVTLASQQIIAFMVLSKKTPLAKEAQTAAFTAISGNEKAAPSIKTGSLKTRRTEK
                     IEI"
     gene            complement(507..893)
                     /locus_tag="PKW73_05945"
     CDS             complement(507..893)
                     /locus_tag="PKW73_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNA72856.1"
                     /translation="MSEKKVITLICERAADLDSVCSAQGEVKDIDGLHVVKLPCSGMI
                     QPLMIEAALKAGAAGVIVTGCQLGDCYYREGNRMIRERLLGERPPGLKKTVDRRRVLA
                     LWLARPQKDRFMSEAKEFVGYVRGLE"
     gene            complement(940..2097)
                     /locus_tag="PKW73_05950"
     CDS             complement(940..2097)
                     /locus_tag="PKW73_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNA72857.1"
                     /translation="MYSSIPKGMTDLTLAIEKATNAVLTNKYNPFYYHGALPQFYLWV
                     LFLSGLLLFAYYIPTIDNAYFSENLVNAWTSVDYITDTLPFGAVVRGIHRYAGDAMVI
                     TIVLHALRVWITDRYRQYRWTQWVTGIVLLLMVLFIGQTGYYLVWDERSLVLTRMTSS
                     ALEALPFVGPGLKAWFLNGAAITNLTLSNFLFIHIGLSFSLLFGLWLHYVRMTRPVIT
                     PPPAINYILTAVLLVFVFILPITSGKMADINAQPTSFEIDWFFLWPYSILASWGTSAF
                     WFVLIGGSVALCLVPFPSLYKAAHPVEAAEVVLNKCTGCSFCSKDCPYQAIEMVPAPA
                     GSRFKLLATVKAYRCSGCGVCVGACAFDAIDLPNLLDTDVTAKIKALAETK"
     gene            complement(2125..2478)
                     /locus_tag="PKW73_05955"
     CDS             complement(2125..2478)
                     /locus_tag="PKW73_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72858.1"
                     /translation="MGKGLNSILAIAGGLLCIVITLALPWAELGMDASFQNWPEWLKW
                     LSINLNVPLPDYSNLANYASEWTAHLKKAGAESRFGDYAYALVMGLAMIASAVGIAAI
                     GREDGEEKTVLPVKK"
     gene            complement(2471..2866)
                     /locus_tag="PKW73_05960"
     CDS             complement(2471..2866)
                     /locus_tag="PKW73_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72859.1"
                     /translation="MEPEKEPDEEMKLVLSANSNSAAEAGAPRGSASEDKPAFKVKLS
                     LKLAVFVMATITLSWFFEGWRQVSEAPTLAGLAYLFGGTTLTIVLVSLYGFFMYAEER
                     SKGGRTRNIPLFDRWYRHLTKQGDKTNRG"
     gene            complement(2866..>3406)
                     /locus_tag="PKW73_05965"
     CDS             complement(2866..>3406)
                     /locus_tag="PKW73_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72860.1"
                     /translation="HPTTGEKPVNQEYWNLHVLLDPGYVARIGEPAAKQDLEELRKLC
                     VETKTPLDFVVKAIPTFETPMGQNGAYLIEQNGKKVSFLSDKEKGAHPTYHGLVDFMV
                     NNRWAWDYCTLVDRLKPGVVKGYPMSVPEVDWYWMIGGALSAAYALVVAMQCKQAQTA
                     EPPKPVASADTKPAETTGS"
BASE COUNT          835 a          872 c          945 g          754 t
ORIGIN      
        1 gagagccggc atctaccggc agtattttat taagtggctg gctgcgacca atcttattca
       61 tgtcgcactt ctgattttga tactctctag actggacatc gctctcttcc ttagacctga
      121 atacatgctt cagattgcgg cggccgtaac tttagcaagc caacaaatca tagctttcat
      181 ggttctcagc aaaaaaacgc cgctagcaaa agaggcccaa accgccgcct tcactgccat
      241 atcgggcaat gaaaaagctg ccccatcgat taagacgggc agcttaaaga ctcggcgcac
      301 cgagaagatc gagatttaga actcggcgac gagtccgaaa caggcgatca atagaatgaa
      361 agcgaagtag agtacgaaga accacgccca tctgtagatt acatctactt ctgtaagttt
      421 ggttttttct gccattttaa gtaatcctcg agaaactcaa cgatagttca agccgcttaa
      481 gctgaactca agccaattta tctattctat tcaagtccgc gcacgtagcc cacgaactct
      541 ttcgcttcag acatgaatct gtctttctgg ggacgagcca accagagtgc cagtacgcgt
      601 cttctgtcga cggtcttctt aagaccggga gggcgttcac caaggaggcg ctctctgatc
      661 atgcggttac cttcgcggta atagcagtca ccaagctggc aaccggtgac aatcacaccg
      721 gcggcgccag ccttgagagc cgcttcaatc atcaaaggct gaatcatgcc gctgcagggc
      781 agcttcacga cgtgcagacc atcgatgtct ttcacttcgc cctgggcgct gcacacagaa
      841 tcaaggtctg cggctcgctc acatatgagg gtgattacct tcttttcgga cataacatta
      901 accttcaggt gtttaccgta aattgaacag aactacttat tacttggttt cggccagggc
      961 tttgattttt gccgtcacgt cggtgtcgag caagttaggc aagtcgatgg cgtcgaaggc
     1021 gcaggcgccc acgcagactc cgcaaccgga gcagcggtag gctttgaccg tagccaggag
     1081 tttgaagcgg gaaccagccg gagcgggcac catttcaatt gcctggtagg ggcagtcttt
     1141 ggagcagaag gaacagccgg tgcacttgtt caggactact tcggcagctt ccacggggtg
     1201 ggcagctttg tagaggctgg ggaaaggcac cagacagaga gccaccgagc cgccaatcag
     1261 cacaaaccag aaggcggaag tgccccagga agccagtatc gagtaaggcc agaggaagaa
     1321 ccagtcgatt tcaaagctgg tgggctgagc attgatgtcg gccattttgc ccgaggtgat
     1381 aggcagaatg aacacgaaga ccaaaagcac ggcagtcagg atgtagttga tagccggggg
     1441 cggtgtgatc accggtctgg tcatacgcac gtagtgcaac cagagaccga agagcaggga
     1501 gaaggaaaga ccgatgtgga tgaaaaggaa gttggacagg gtcaggttgg tgatggcggc
     1561 accgttcagg aaccatgctt tcaaaccagg tcccacaaag ggcagagctt ccagagcgct
     1621 ggaggtcata cgggtcaata cgagggaacg ctcgtcccaa accaggtaat agccggtctg
     1681 accaatgaag agaaccatca agagaaggac gataccggtc acccactggg tccagcggta
     1741 ctgacgataa cggtcggtga tccagacgcg cagggcgtgc agcacgatgg tgatgaccat
     1801 ggcgtcaccg gcgtagcggt ggatgccgcg caccacggca ccgaagggca gggtgtcggt
     1861 gatgtagtct accgaggtcc aggcgttcac caggttctca gagaagtaag cgttgtcgat
     1921 ggtcggaatg tagtaggcaa acagcaaaag cccggacaag aacagaaccc acagataaaa
     1981 ctgaggcaga gccccgtggt aataaaaggg gttgtactta ttggttagaa cggcattggt
     2041 cgctttctct attgctagag tcaggtcggt catgcccttc ggtatcgacg agtacatctt
     2101 tgaggatctc ccaactttaa ctagttactt cttcaccggt aaaactgttt tttcttcgcc
     2161 gtcttcgcgc ccgatggcag cgatgccgac ggcggaggca atcatggcca gacccatcac
     2221 cagagcgtaa gcataatcgc cgaatctaga ttcagcgccg gctttcttca ggtgcgcggt
     2281 ccactcgctg gcgtaatttg ccagattcga gtagtccgga aggggcacgt tgaggttgat
     2341 ggatagccac ttcaaccatt cgggccaatt ctgaaatgag gcatccatac caagctcagc
     2401 ccaaggcaaa gccagtgtga tgactataca aagcaaacca ccagctatcg ctagaatgct
     2461 gtttaatccc ttacccacgg ttcgttttgt ctccctgttt ggttaagtgc ctgtaccaac
     2521 gatcaaatag aggaatgttg cgggtgcgac cgcccttgga gcgttcttca gcgtacataa
     2581 agaagccgta gagactgacc agaacgatag tcagtgttgt accgccgaag agataggcca
     2641 gaccggcaag ggttggagct tcgctaacct gtctccaacc ttcgaaaaac cagcttagcg
     2701 tgatggtggc catcacgaaa actgctagtt tgagcgagag cttgactttg aaagcaggct
     2761 tgtcttctga agcggaaccc cttggggctc cggcttcagc cgcactattt gagttagcgc
     2821 tcaagacaag tttcatttct tcgtcaggtt ccttttctgg ctccatcagc ttccggtagt
     2881 ttcagccggc ttggtgtcgg cggaagcaac cggcttgggc ggctcagctg tctgggcttg
     2941 cttgcactgc atggctacca cgagagcgta ggcagcagac aatgcaccgc cgatcatcca
     3001 gtaccaatca acttcaggca ctgacatcgg gtagcctttg accacaccgg gcttcagtct
     3061 atccaccaga gtgcagtagt cccaggccca gcggttgttg accatgaagt cgaccaagcc
     3121 gtgataggtg gggtgggcac ccttctcttt gtcggagagg aagctgactt tcttgccgtt
     3181 ttgttcaatc aggtaggcac cgttttgacc catgggggtc tcgaaggtgg ggatggcttt
     3241 gacgacaaaa tcaagaggtg tcttggtttc gacgcagagc ttccgtagtt cttccagatc
     3301 ttgcttggcg gctggctcac cgatacgagc cacgtaacca ggatctaaga gcacatggag
     3361 gttccagtac tcctggttga ccggcttctc gccggtggtg ggatgc
//
LOCUS       DAOQNZ010000261         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13702_length_3397_cov_3.479653, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000261 DAOQNZ010000000
VERSION     DAOQNZ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13702_length_3397_cov_3.479653"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PKW73_05970"
     CDS             complement(<1..851)
                     /locus_tag="PKW73_05970"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA72861.1"
                     /translation="MKIDIHTHLLPENLPDWKSEFGYGGFIRLEHHKPCCARMMRDDG
                     KFFREVEENLWSGAARVKDMEQTGVAKQVLSTVPVMFNYWAKPKDGLNSSRFLNDHLA
                     EVVAQNPDRFYGLGTLPLQDTDMACAELVRCVKELGMQGVQIGSHVEDMNLSDERLFP
                     VFAEAEKLGAAIFVHPWDMMGEARMTKYWLPWLVGMPAEVSLAISSMIFGGVFERLPK
                     LRVAFAHGGGAFPFTLGRIEHGFEARPDLCAVDNPHPPSHYVGKFFVDSLVHDGRALK
                     YILDLFGP"
     gene            complement(890..1405)
                     /gene="nbaC"
                     /locus_tag="PKW73_05975"
     CDS             complement(890..1405)
                     /gene="nbaC"
                     /locus_tag="PKW73_05975"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HNA72862.1"
                     /translation="MGRLSTLNFKKWIDDNRHLLKPPVGNKLIWEDREFIVMVVGGPN
                     KRTDYHVNEGEEFFYQVEGDMVLKVFDEGKLQDVPIKEGEIFLLPPGVPHSPQRPEKT
                     VGLVIEKKRQEGEQDGFVWFCQNCGEKLYEEYFHLTNIVTQFQPIFERFYASPEHTTC
                     KKCGTRTTKPV"
     gene            complement(1778..>3397)
                     /locus_tag="PKW73_05980"
     CDS             complement(1778..>3397)
                     /locus_tag="PKW73_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72863.1"
                     /translation="PAFALSACLLMGDSSSTLAQTGAASVSGAAAQTGSKSVNLAGLY
                     AQGNYTGALAAARSELAAAASNRTTRGKALSNLSAVLRFTGSPQEALDAALEAHELCP
                     NDAVVLANLATLTCAYGDRDQALPLYQECLRLSPRDWLSHMGLAQALAFSYSADLTDG
                     IKAPDQSVSQLQQARGELEKAQSLLAEIKDAPGNYWLQLGDSNLLLRQYSRALFCYEQ
                     GQRSPGRAEERSLCLEKALQSRLARGDGAQTAALAARVLQNPVVDAETYKLILVRLVP
                     SLPTDAGGRLLTAVEKRLRSDPLREEDAALKLSLGRRLEESGRLGEARKYFLAAYKSR
                     PENLSFALALSRCLFLDGERQRSQQIVVDLFKSVRNKEMDAGNLVALRAAGAALELTR
                     ADSGDLAYSLRQALSAATAGGGSAGGGNSGNSGKLYVSRATVGDWQCACNMNSIRYVL
                     LKRSGVVYAHLQPASPGKAMVLLRLPKPPGDAARLWGGLEKMVKVKSISPLTTLNDFP
                     NFAKTILDAEEAYYEPVVSVYNFAMPPMRIQ"
BASE COUNT          810 a          945 c          925 g          717 t
ORIGIN      
        1 ggaccaaaga gatcaaggat gtatttgaga gcacgcccgt cgtgcacgag gctgtccaca
       61 aagaatttgc ccacataatg gctgggtggg tgaggattgt ccacggcaca gagatcaggt
      121 ctggcctcga agccatgctc gatacgacca agggtaaagg ggaaggcacc gccaccatgg
      181 gcgaaggcaa ccctcagctt gggcaagcgc tcaaatacac cgccgaaaat catggaggaa
      241 atggccaggg aaacttcggc aggcatgcca accagccagg gtagccagta tttggtcatg
      301 cgtgcttcac ccatcatgtc ccagggatgc acgaaaattg ccgcccccag tttctcggct
      361 tcggcaaaaa cgggaaagag gcgctcatcg gaaaggttca tatcttccac atgggagcct
      421 atttgtaccc cctgcatgcc cagttctttg acgcagcgca ccagttcggc acaagccata
      481 tcggtgtctt gcaacggcaa ggtgccaagc ccgtaaaagc gatcgggatt ttgagcgaca
      541 acctcagcca gatggtcgtt gagaaagcgc gatgagttaa gaccgtcttt gggtttagcc
      601 cagtaattga acataacagg aacggtagaa agcacctgct tagccacccc ggtttgctcc
      661 atatccttga cacgggcggc gccgctccag agattctctt ccacttcccg aaaaaactta
      721 ccgtcatcac gcatcatgcg ggcgcaacat ggtttgtgat gctctaggcg aataaagccg
      781 ccataaccga attcactttt ccagtcaggc aaattttccg gcagcagatg ggtgtgaata
      841 tcgattttca aaactggtca gcctccgagt gtcgctcagc gaaccgcatt tataccggct
      901 ttgtagtacg ggtgccgcat ttcttacagg tcgtatgctc aggtgaggca tagaaccttt
      961 caaaaatggg ctgaaactga gtgacgatgt tggttagatg gaagtactct tcatagagtt
     1021 tctcgccgca gttctggcag aaccagacga acccgtcctg ttccccttcc tgacgcttct
     1081 tctcgatcac cagaccgaca gttttttcag ggcgctgggg gctgtgcggc acaccaggcg
     1141 gcaaaaggaa gatctcgcct tctttgatag gaacatcttg cagtttgcct tcatcgaaga
     1201 ccttcaaaac catgtcgcct tcgacctggt agaagaattc ctcgccctca ttcacatggt
     1261 agtcggtacg cttgttgggt ccgcccacta ccatgacaat aaattcgcgg tcttcccaga
     1321 ttagcttgtt gcccacaggc ggtttcaaga ggtgccgatt gtcatcaatc catttcttga
     1381 agttgagggt tgaaagtcgt cccacaaata cctcccgcag ccagcactta accaagacga
     1441 tgttaaggga aacaaagttc aggaaaagag gcgtcccaag cgatgaattt tcttgtggaa
     1501 acagccccaa gacctcacgc tgtaagtcta cagagggaaa gtgcggagtg gggataaatc
     1561 agataagctt tatatgcaca aaaggtggta gtgacggcac tgaagatggc atttttcaaa
     1621 aaacgtccca gatccgctct gcaaattcgg cttgttcgag agttttcagg ccactatgac
     1681 ggaaaagcct gaacagcacc gattcagatt gcaaatctct aatatgccat ggtttccagt
     1741 ttgccccctt gcattccccg gcgttaaaag cggcttttta ctgaatgcgc atgggcggca
     1801 tggcaaagtt atagacactt acaaccggct catagtaagc ctcttccgcg tccagaatgg
     1861 ttttggcaaa gttggggaaa tcgttcagcg tggtcaaggg actgatggac ttgaccttca
     1921 ccatcttttc aagcccgccc catagtctag ccgcgtcgcc ggggggcttg ggcaagcgaa
     1981 gcagaaccat ggccttgccc ggagaggccg gttgcaagtg ggcatagacg acgccgctgc
     2041 gcttgagcag cacatatcgg atactgttca tattgcaggc gcattgccaa tcgcctactg
     2101 tggctcggct gacatataat ttaccactgt taccactgtt accgccgccg gcactgccac
     2161 caccggcagt agcagcactg agagcctgac ggagtgaata tgccaggtcg ccgctatcgg
     2221 ctctggtcag ctccagagca gctccggctg ccctgagcgc cactaaattg ccggcatcca
     2281 tctctttatt gcgcaccgac ttgaagagat ccaccacaat ctgctgcgag cgctgccgtt
     2341 cgccatcaag aaagaggcag cgggagaggg ctagagcaaa ggagagattt tcaggacgac
     2401 ttttgtatgc tgccagaaag tactttctcg cctcgccgag ccgacccgac tcttccagcc
     2461 gcctgcccag ggagagcttg agtgccgcat cttcttcacg gagcggatca gaccgcaatc
     2521 gcttttcaac ggcggtgaga aggcgcccgc ccgcgtcggt cggaagcgag ggcaccagtc
     2581 gaaccaggat aagtttgtag gtttccgcgt ccaccacagg attctgcaat acccgagccg
     2641 ccagagcagc ggtctgggcg ccatcacccc gagccagcct ggactgcagc gccttctcca
     2701 ggcaaaggct gcgctcctcg gctctgccgg gcgaccgctg cccctgctca taacagaaca
     2761 gggcacggct atattgcctg agcaggaggt tactgtcccc cagttgcagc cagtagttgc
     2821 cgggagcgtc cttaatttcc gcaagtagac tctgcgcttt ctccagttcc cccctggcct
     2881 gctgcagctg gctgacagac tggtcgggcg ccttgattcc atcggtcaaa tcagccgaat
     2941 agctgaaagc tagcgcctga gccagaccca tatgggacag ccagtcacgg ggagaaagcc
     3001 tgaggcattc ctgatagagc ggcaatgcct gatcgcgatc accataagca caggtgagag
     3061 tggccagatt agcgagaacg acggcgtcat tgggacagag ttcgtgggcc tcaagggcgg
     3121 catcaagggc ctcctgagga ctaccggtga agcgcaacac cgcagaaaga ttgctgagag
     3181 ccttgccccg cgtggttctg ttggatgcgg cagcggcaag ttcggacctg gccgcggcaa
     3241 gagcaccggt ataattcccc tgagcgtaaa gcccggccag atttacactt ttcgaaccgg
     3301 tttgggccgc cgccccggag acacttgccg ccccggtctg agcaagggtt gatgaactat
     3361 cacccatcaa aaggcaggcg gagagagcaa aagcagg
//
LOCUS       DAOQNZ010000262         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13713_length_3396_cov_3.787189, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000262 DAOQNZ010000000
VERSION     DAOQNZ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13713_length_3396_cov_3.787189"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKW73_05985"
     CDS             complement(<1..404)
                     /locus_tag="PKW73_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNA72864.1"
                     /translation="MNSQESRSGAQTTQGNWRERLYNPYTVMWLVVLMYTIKVATKLS
                     AGYLTNSPVFISDGYHNLSDILQAFAVMVVVYMARRPPNTDYPLGKSNIEFFSSLVIG
                     LSLLVLGAQFALQSLVGLLYYLPQADQAVRAY"
     gene            complement(401..1168)
                     /locus_tag="PKW73_05990"
     CDS             complement(401..1168)
                     /locus_tag="PKW73_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA72865.1"
                     /translation="MNPKQTKLIIGVDYFRNIDRVPNLAFARALMQYLDVELIHVRAT
                     APGADMTHLAEEQATRIRQDVSRLTQVPEDCVVIVPDFPPGALIIDNFPDRNRQNLIW
                     LAPMDERGLTTRHRGSANDASIMVPFGNGNSCLKALQLAAPIARGSGAALLFYHTTWK
                     IPGLASVKADDHMCREAIAIRKALEEEATAQSLPFRTLIETADDVADGIVQAAVLQRS
                     RLIVMPRSQTVEVGDYCLRVFDKSPVPVITIGKEVTS"
     gene            complement(1582..2007)
                     /locus_tag="PKW73_05995"
     CDS             complement(1582..2007)
                     /locus_tag="PKW73_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72866.1"
                     /translation="MNQNKQNGNPYPLLPLILISLSLLTIVGVGLFSQAPGQASIPPA
                     CQVSVTITPQLDNVFVIEASGMLEAPDVVDIGGEAPGLTLVSGNDAFGLSFTEKPALA
                     STASQMVGKCVKVSGVLKEVGGVERPPRKVIDVTSLTSN"
     gene            complement(2122..2610)
                     /locus_tag="PKW73_06000"
     CDS             complement(2122..2610)
                     /locus_tag="PKW73_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72867.1"
                     /translation="MKNTTVALPVNAVDGNWNTLTNWGLTQVGEPNSLTVMTELPAGW
                     KLSERNSVEAQSAPTGQQMQYDLIDEYGRKRADVVIITAPREKAFISLCRRYVIAPTP
                     ESDTTNVGFRVLDGGSEVFRTAQVACSTTENDKRNQIRAVASQWLSEHYPDYHNTAAY
                     WD"
     gene            complement(2640..2984)
                     /locus_tag="PKW73_06005"
     CDS             complement(2640..2984)
                     /locus_tag="PKW73_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72868.1"
                     /translation="MKAQINLDADDVIELIKAVLLRKDCRVTADPILRGINSRNMRIT
                     VSFIQNGALSTTTLDKDELKNMIADDFRLRGYSVMRPFVDLLHGNRQSFPPGMVGAFC
                     AIVNINLPYQLN"
     gene            complement(3022..>3396)
                     /locus_tag="PKW73_06010"
     CDS             complement(3022..>3396)
                     /locus_tag="PKW73_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72869.1"
                     /translation="SYSAHTWAVVLGLSSAAATGYYSYQVSDSVVTSVVLAAAVADIA
                     AYVVAPLSYLAVRDVTAGVTPALAKFARDSFDAIWSIYVRIWAAIGTRFAIVMTILAP
                     IFASIINACRAVSATVSRIFGH"
BASE COUNT          727 a          830 c         1027 g          812 t
ORIGIN      
        1 atgtaggcgc gcacggcctg gtcggcttgc ggcaggtaat agagcagacc caccagcgac
       61 tgcagggcga attgcgcgcc cagaaccagt aagctcagcc cgatcaccag ggatgagaag
      121 aattcgatat tgctcttgcc cagggggtag tcggtgttgg gtggacggcg tgccatgtag
      181 acaaccacca tgactgcgaa agcctggagg atgtcggaga ggttgtgata gccgtcggag
      241 atgaagaccg gtgagttggt cagatagccg gcggaaagtt tggtggccac cttgatggta
      301 tacatcaaga ccaccagcca catgaccgtg taggggttgt agaggcgctc acgccagttt
      361 ccctgtgtgg tttgagcgcc gcttcgggat tcttggctat tcatgatgtg acctccttgc
      421 cgatggtgat aaccggcacc ggcgacttgt cgaagacgcg caagcagtag tcgcctactt
      481 ccacagtctg gctgcggggc atgacgatca gccggctgcg ctgcaagacg gctgcttgca
      541 cgatgccgtc ggccacatca tcggcggttt caatcagggt gcggaaggga agggactggg
      601 ctgtcgcttc ctcctccagt gccttgcgga tggcgattgc ttcccggcac atgtggtcgt
      661 ctgctttgac cgaggcgaga ccgggaattt tccaggtagt gtggtagaag agcagtgcgg
      721 cgccactacc cctggcaatg ggagccgcca gttgcagcgc cttcaggcag gagttgccgt
      781 tgccgaaggg caccatgatg gaagcatcgt tggcgctgcc ccggtggcgt gtggtcagtc
      841 cccgctcgtc catgggagcc agccagatca ggttctggcg attgcggtcc ggaaagttgt
      901 cgataatgag ggcaccgggc gggaagtccg gcacgatcac cacgcagtct tccggtactt
      961 gagtgagacg cgacacgtcc tggcggatgc gtgtggcttg ttcttcagcc aggtgagtca
     1021 tgtctgcccc tggcgcggtg gcgcggacat ggatgagttc cacatcgagg tactgcatga
     1081 gcgctcgggc aaaggccaga ttcggcacac ggtcgatgtt gcggaagtag tccacgccga
     1141 taatcagctt ggtttgtttt gggttcatag taaactccca gaagagttag acaatcaaag
     1201 actgccgccg ctgaagcggg gctgagtgga gatgaattag tagcgttagt ggcgtatggt
     1261 cggtttttcg aagggtggcc tttgactttc aagcaatatg gatggaactt tctcaatcta
     1321 gaaagttgtt gaaagctatt gccattaaga aaaaggcaaa gtgtctgtcg aaaagggcca
     1381 aaaagttaaa aaagaacctg aggatggtgt cttgcttagc ttcggtaaag ttggttccag
     1441 agaagatggg gcggcgttta gcagttctcc ggtcctatca ggtgtggttt ctcaagtgct
     1501 tcaaagctta agaacagcct aaacgcctct tctaaattgc cgctcatgta cctcgctcta
     1561 gctggtgcaa gctcgctcag cttagttgct ggtcaggcta gtgacatcta taacttttct
     1621 gggcggtctt tcgacgcctc ccacttcttt caggacgccg ctaactttta cgcattttcc
     1681 gaccatttgg ctagccgtgg aagcgagagc gggcttttcg gtgaaactaa gaccgaaggc
     1741 atcgttgccg ctcacgagag tcagacccgg tgcttcaccg ccaatgtcga ctacgtccgg
     1801 tgcttccagc atacctgaag cttcaatgac aaagacattg tccaactgcg gcgtaattgt
     1861 taccgaaacc tggcatgccg gtggaatgga ggcttgaccg ggtgcctggg agaagagacc
     1921 gacgccaact atggttagca agctgagtga aatcaggatg agtggaagaa gtggataggg
     1981 attgccgttc tgtttgtttt ggttcatgga ggttgctctt ttgagttggg aaggagcgac
     2041 accggacctc tcctgggtgt cgctcctggt gacagactag tcttgccctt gagcaagatc
     2101 agtgctgcgg ttcgccgtca atcagtccca gtaggcggcg gtgttatggt agtccggata
     2161 atgttcggac agccactggc ttgcaactgc gcgaatctga ttgcgtttgt cattttcggt
     2221 agtgctgcaa gcaacctggg cggtgcgaaa gacttcgctg ccgccatcga gaactcggaa
     2281 accaacgttg gtggtgtccg attccggggt gggagcaatc acatagcgac ggcagaggct
     2341 gatgaatgcc ttctcccgcg gtgccgtgat gatcaccaca tcggcgcgct tgcgaccgta
     2401 ttcgtcgatc aggtcatact gcatttgctg accagtggga gcgctctggg cttcaacgct
     2461 gtttctttcg ctgagcttcc aaccagccgg cagctcggtc ataacggtaa gcgaattagg
     2521 ctcgcccact tgagtcagac cccagtttgt gagggtattc cagttgccgt cgacagcatt
     2581 tacaggtagg gctacggttg tgtttttcat aatactattt ctcctgaatt tacttcggat
     2641 tagttgagtt ggtaaggcag gttgatgttg acgatggcgc agaatgcacc aaccatgcct
     2701 ggtggaaagc tctgtctatt gccgtgaagc aggtcgacaa aggggcgcat cacagaatag
     2761 ccgcgtagac ggaagtcatc ggctatcatg ttcttgagct catctttatc cagagtggtc
     2821 gtgcttaagg caccattttg aatgaacgaa acggtgatgc gcatgtttct ggaattgata
     2881 ccccttaaga ttgggtcagc cgttacccgg caatcttttc tcagcaagac ggctttgatc
     2941 agttcgatca catcgtctgc gtctagatta atttgagcct tcaatgggtc tccctcgctg
     3001 gcggttatat agatgctcgt tttagtgtcc gaaaattctg cttaccgtgg cgctcactgc
     3061 tcggcaggca ttgatgatgc ttgcgaagat aggcgccagt atcgtcatca caatggcaaa
     3121 acgtgtgccg attgctgccc agattctgac gtagatggac cagatggcat cgaagctatc
     3181 tctggcaaat ttggccaggg cgggagtgac tccagcggtg acgtcgcgca cggccaggta
     3241 agagagtggt gctaccacat aagcggcgat gtctgccact gctgcagcta gaactacgga
     3301 cgtgacaacg ctgtcgctga cctgataaga gtagtagcct gtggcggcag cggaactcag
     3361 tcccagaaca acggcccagg tgtgggcgct atagct
//
LOCUS       DAOQNZ010000263         3383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13797_length_3383_cov_4.154748, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000263 DAOQNZ010000000
VERSION     DAOQNZ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13797_length_3383_cov_4.154748"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PKW73_06015"
     CDS             complement(<1..1177)
                     /locus_tag="PKW73_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72870.1"
                     /translation="MIIRWLSSEIRTFAYFKNVPLMACLFGLGVGLALSKSPRDWQKW
                     VPWSLLAVVSLICFADPLKLVHITIVDPTEFYIVGPIAWKAMDATRPLDYVLQTVKGY
                     GVLLGVYYLLVLTFVGIGQRIGSLFDKLKPLQAYTVDIAASLIGVLAFSLMSFFSLPP
                     AWWMLTVAVMGAVIFRKPAQIAALVLATVIGFATTNPEVTWSPYYRISIRDGIIAADG
                     DSPEFFYGYNIDVNHDGIMGAYDNRPESLKRLTPNQREKVLQYYDMLYALIGDKPRTA
                     MIIAAGAGNDLACGLRHGLTAIDAVEIDRTFVAKGKALHPEKPYDNPAVTVHIDDARA
                     YMQRCAKTYDLVDFAYLDAHTAFSSMSSIRLDNYIYTEESFRNAKRLLNERGILAVTF
                     "
     gene            complement(1429..1932)
                     /locus_tag="PKW73_06020"
     CDS             complement(1429..1932)
                     /locus_tag="PKW73_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72871.1"
                     /translation="MKSIAVVVVLLLGFAAVPAALGQKPTGPAANPPSPEERAAQIAK
                     IRREYQTPGVKVASTQPSNFPLPPYTSNVTDKGFVHSTKGTPAAAVTICTKDAPDVVY
                     KWYLDACKRAQLSTSTPTAQAMDKMGRQGDLFLITAENPKQKMNITCVRNAKDNGTII
                     SINWFLK"
     gene            2245..>3383
                     /gene="pyk"
                     /locus_tag="PKW73_06025"
     CDS             2245..>3383
                     /gene="pyk"
                     /locus_tag="PKW73_06025"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNA72872.1"
                     /translation="MRNTRTKIIATIGPSCRDPQVIGEMVNAGMDVARINCSHASAEF
                     IQDVVADIRELSSRMEHPIGILLDLSGPKLRTSKLKNNEPVMLEKGKTFTLTNRKVEG
                     TNEIVGTNYAPLSQEVKPGDSILLDDGLIELKVKSTNDTDVECEIIIGGSLKNHQGIN
                     IPGVRLSIPALTEKDRQDLKIGLACEVDFVALSFVRDAQDILELKEEIGDHWPPVMVI
                     AKLEKPEAVENLEEILNVTDAVMIARGDLGVELPPEKVPPAQKLITRRANAKGKPVIT
                     ATQMLDSMANNPRPTRAEASDVANAIFDGTDAVMLSEETAMGAYPVEAVHMMDKIAFE
                     AENSQDRSKLHEHELYSSAHAVAHAACAMAVDMDAKAIATFTKSGS"
BASE COUNT          910 a          909 c          854 g          710 t
ORIGIN      
        1 agaaggtgac tgctagaatc cctctttcat tgagcagtcg cttggcattt cggaagctct
       61 cctcggtata aatgtagttg tctaggcgaa tggaagacat ggaagagaag gccgtgtgag
      121 catctagata agcgaaatca accaaatcat aggtcttcgc gcaacgctgc atgtacgcac
      181 gtgcatcatc tatatgaacg gtcacggctg gattgtcata cggtttttcc ggatgcaaag
      241 ccttgccttt ggcaacaaag gttctatcta tttcaacagc atctatagcg gtcaaaccgt
      301 gcctgagacc gcaagcgagg tcattgccgg cgccagctgc aatgatcata gctgtgcgag
      361 gcttgtcgcc gattagggca taaagcatat catagtactg gagcaccttt tccctttgat
      421 tcggcgttag acgcttcaaa gactccggtc tattgtcata ggctcccatt atgccgtcat
      481 gattgacgtc gatgttgtat ccataaaaga actccgggct atcgccgtca gcggcaatga
      541 taccgtctct gatactgata cgatagtaag gagaccaggt aacttccggg ttagtcgtag
      601 caaaaccaat cactgtagcc aagactagag cagcaatttg tgccggtttg cgaaaaatga
      661 cagcacccat gactgcaacg gtaagcatcc accaggcagg cggtagagag aagaaggaca
      721 taagcgagaa agccaaaacg ccgatgaggc tggcagctat gtctaccgtg taggcctgta
      781 gcggcttgag cttatcaaaa agtgagccta ttcgctgacc aatgccgaca aaggtaagca
      841 ccaaaaggta gtagacaccc aaaagtactc catagccctt tacagtctgc aaaacatagt
      901 ccagcggtct cgtagcatcc atagccttcc aagctatagg accgacaata taaaattctg
      961 ttggatctac gattgtgatg tgaacaagtt ttagaggatc ggcaaaacag atcaacgaca
     1021 caacggcgag aagcgaccag ggaacccact tttgccaatc tctcggagac ttgcttaaag
     1081 ccagtcccac tcccaaacca aacaagcatg ccatgagcgg cacattcttg aagtaagcga
     1141 aggtgcgtat ctcggaggaa agccaacgta taatcatcag ctctagaaaa agcgagaaga
     1201 agcaaatccc cgcaagtgcc agcaacgttt ttcgaactgt agacatctat gaataaaaac
     1261 cgtgaacatt tggatgagac atgaaacccc gggagtaggt tatcattact attggtaaaa
     1321 ttcaaaggta tgaaagggca aaatttctct tggtctgatc cgcgtcaagc gctggacgga
     1381 agtgcaggtt cccggtatgt cggtcttccc ataaggctgg agcggtagct acttaaggaa
     1441 ccaattgata ctaattatgg tgccattgtc tttggcattg cgcacacagg tgatattcat
     1501 tttctgcttg gggttttcgg ccgtaatcaa gaaaagatcc ccttgtctgc ccatcttgtc
     1561 catagcctga gcggtaggcg tgctggtgga aagctgagcc cttttgcaag catccaggta
     1621 ccacttgtac accacatccg gtgcatcctt ggtgcaaata gtgacggccg ccgccggtgt
     1681 ccctttcgtg gaatgaacaa agcccttgtc agtgacatta ctggtgtaag gaggcagggg
     1741 gaaattggag ggctgggtgg aggccacctt cactccgggg gtctggtact cccttctgat
     1801 tttggcaatc tgcgcggccc gctcttccgg cgagggcggg ttggcggccg gtccggtggg
     1861 tttctgcccc agggcggcag gtacggcggc aaaacccaga agaagaacaa caaccacggc
     1921 gatagatttc ataaagcacc catgaaagca actgcttgcc aatgatagca agccaccgca
     1981 acgggaaggg accccggcaa ggaaagcagt gacaatcgcc tgttgacaag ccggggcgca
     2041 attatgaaat tgcctgatta ccaccttaaa accttgatat ttcttcaacc aaggctcctg
     2101 cccctttcaa cctgcctgcg ccctgttgta cctttgacct ctgagccaga cttagaacat
     2161 aagagcattt gcatgaaccc taccggactt cagtcggaat tgaaccgacc agcacaacct
     2221 tcacatagga cgagccattc caccatgcgg aacacccgta ccaagatcat cgccaccatc
     2281 ggcccctcct gccgggatcc ccaggtcata ggcgagatgg tcaatgccgg aatggacgtg
     2341 gctcgcatca actgctccca tgccagcgcc gagttcattc aagacgtggt tgccgacatt
     2401 cgcgagttga gcagccgcat ggaacacccc atcggcattt tgctcgatct ctccggtccc
     2461 aaactgcgca ccagcaagct caaaaacaac gaaccggtga tgctggagaa aggcaagaca
     2521 ttcaccctca ccaaccgcaa ggtagaaggc acaaacgaaa tcgtaggcac caactacgcc
     2581 cccctttccc aggaagtgaa gccaggcgac agcatcctgc tcgatgacgg tcttattgaa
     2641 ctgaaagtta agtcaaccaa tgacaccgac gtggaatgcg aaatcatcat cggcggtagc
     2701 ctgaaaaacc atcagggcat caatattccg ggcgttcgcc tctcgattcc ggccctgact
     2761 gaaaaagaca ggcaagattt gaaaatagga ctggcctgcg aagtagactt cgtagcgctc
     2821 tctttcgtga gagacgctca ggacatcctc gaactgaaag aagagattgg cgaccactgg
     2881 ccgccggtca tggtcatagc caaactggaa aagcccgagg ccgtcgaaaa tctggaagaa
     2941 attctcaatg tcaccgacgc cgtcatgatc gcccgcggcg atctgggcgt ggagttgccg
     3001 ccggagaaag tgcccccggc tcagaaactc attacaagaa gggccaacgc caagggtaag
     3061 ccggtcatca cggccaccca gatgctcgat tctatggcta acaaccctcg ccccacccgc
     3121 gccgaagcct ccgacgtagc caacgccatc ttcgacggca cagacgccgt catgctttcg
     3181 gaagaaacgg ccatgggcgc ttatccggtg gaagcagtgc acatgatgga taagatcgct
     3241 tttgaagcgg aaaacagcca ggaccgctcc aagttacacg agcatgaact ctattcttcc
     3301 gcccatgccg tcgcccacgc cgcctgcgcc atggctgtag acatggatgc taaagccatc
     3361 gctactttca ccaagtccgg ctc
//
LOCUS       DAOQNZ010000264         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13807_length_3382_cov_3.733694, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000264 DAOQNZ010000000
VERSION     DAOQNZ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13807_length_3382_cov_3.733694"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKW73_06030"
     CDS             complement(<1..442)
                     /locus_tag="PKW73_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72873.1"
                     /translation="MKSSQDSEKKEQSKENEQGELKQQEKPPAPIDSSALEDKTSPTG
                     TMPADIEAEKLATAKKLEESNKKDASLGTAPIEIKECESATVSDNRPLTPRRQDVGDR
                     LEEETRSKQAQELFAGKGTFFTRNLSERERLIAVRDSYDRIAQIL"
     gene            565..1065
                     /locus_tag="PKW73_06035"
     CDS             565..1065
                     /locus_tag="PKW73_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72874.1"
                     /translation="MVDVFTAVSTGSESYYPSCRQPQFSLPCLVLPPPQSCLEASAAN
                     LELTAADILSVLALELEGTFTGAEILNTELVLALALRLPTGVDVEKSVGLSLDCLYWL
                     HSRAFKLLALSCSCRLDAYALEFLSDSGLAVLDGLDLSNLWPGSLGVLTWGTLGDTPV
                     FVQDGD"
     gene            complement(1072..1290)
                     /locus_tag="PKW73_06040"
     CDS             complement(1072..1290)
                     /locus_tag="PKW73_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72875.1"
                     /translation="MNLTSYSFPPLNQQGASERDSGALLKMTADAYNTTRPLNADRFV
                     SSEAEDILKGFALDVNYADSAQVVFAVL"
     gene            complement(1388..>3382)
                     /locus_tag="PKW73_06045"
     CDS             complement(1388..>3382)
                     /locus_tag="PKW73_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72876.1"
                     /translation="TSRQALGKYDQGQAEQLEEFIVASIEPRLKDPNFTGAASADYYV
                     TRALMKGDNLGSADASALNYALKNVADEPQFRQALFKELAARGKEGTLPEKTAQLWFN
                     EIGERLKANPKERTQAYQDFLDNTPAEIKNKITARPEAQEALRKAGLLEMPESTAVVE
                     SRHWSQEELPQTVRHLEETKADIIRKPEFRQTHSMGERSQLAVWNFLTSEKDGILPRL
                     KQQNKISQDWQIYPSEAGSPLDRAGADYILVNKKTGDVHFLDATANSDKKNVFKIRED
                     GVIHIDNEIFDVSGALKVDDLDVDLAARARDFKPELEQQILSLVQSPSALRLGADGPP
                     LPSLTRASDVDATRQLNALIDWAEQKSKLADMEADKALYHDMAATLGRARNYSNMKAT
                     EHASPTLKEQLDSAARGELLRYAVARIKNGTYGKSAQTAPTADVQLHKDGRIVLKTTE
                     GAYYHGGALAESVEGARRLFLDRSALARSLSNKQIEDLGGNAKALKTLSGAERQAAIE
                     KEMKQNATFRRNVEQASQVMISDRSIISTGLAQGSGAAVLNDNIIGRLRTRKDGDLLG
                     QTSAPLKPKAAETAVSEPLDKTMPKAAQILREWREPPGTPEQASANIAEILDLVAGES
                     KALPAAEHASLVKLRDAYKDPNHPDHARAVKQVHDLMLAD"
BASE COUNT          557 a          901 c          955 g          969 t
ORIGIN      
        1 gaagaatctg ggcaattcga tcgtaagagt cgcgcaccgc tattagtctt tccctttcac
       61 tgaggtttct ggtgaaaaag gtgcctttac cggcaaacag ttcttgcgct tgcttgcttc
      121 tggtttcctc ctccagtctg tcgcccacat cctggcggcg cggcgtcagt ggtctgttgt
      181 cgctaacggt ggcgctttcg cactctttga tttcaatggg ggcggtaccg agagaggcat
      241 ccttcttatt ggattcttcc agcttcttgg ccgttgccag cttttctgcc tctatgtcgg
      301 caggcatggt gcctgtagga cttgttttgt cttccagggc cgagctgtct attggtgcgg
      361 gcggtttttc ttgctgcttc agctcgccct gctcgttttc cttagactgc tctttcttct
      421 ctgagtcctg actgcttttc atttatagac ttgagcccaa ttgtgttgcc ttaatctgct
      481 tgtttgccac ccatttgtag aattccaccg ggcagtggga atgaaacttt tacgggtgat
      541 ttcggggcga cttacgaggc ttccatggta gatgtcttta ctgctgtttc cactggttca
      601 gagagctatt atccctcttg ccggcagccc cagttttctt tgccctgcct tgttctgccg
      661 ccgcctcaat cttgtctgga agcctccgct gccaatctcg aattgaccgc cgccgacata
      721 ctttctgtgt tggccctgga actggagggt actttcaccg gtgccgagat cttgaacact
      781 gaattggttc tggccctggc tctcagattg cctaccggtg tagatgtaga aaagagtgtc
      841 ggtttgagtc tggattgtct ttactggtta cacagccgtg cctttaaact attggcgctc
      901 tcctgtagtt gtcgccttga tgcttatgca ctggagtttc tcagtgactc cggtcttgcc
      961 gtcctcgatg gtttggactt atccaacctc tggcccggca gtctgggcgt gctgacctgg
     1021 ggtacgctcg gtgacactcc ggtgttcgtg caagacggcg actgatgggt tttaaagtac
     1081 ggcaaagact acctgtgcgg agtctgcata atttacgtct agagcgaagc ccttcagtat
     1141 gtcttcggct tcactggaaa cgaatctatc ggcattgagc ggtctggtcg tgttgtaagc
     1201 gtcggcggtc atttttagaa gagcgccgct atcacgttct gaagcgcctt gctggttaag
     1261 aggcggaaaa ctgtaggagg taaggttcat gtgcggtccc tcagttaaga aaatgccttc
     1321 aggtgtttct cctgcctggc tcctctaatc taagggggca ttgtttcaaa gctattgcaa
     1381 agtcagttca gtctgccagc atgaggtcat gcacttgttt gactgcccgg gcatgatcag
     1441 gatgattggg atctttataa gcgtctctca gtttgaccag actggcgtgt tctgcggcag
     1501 gaagggcctt gctttctccg gcaacaagat ccagtatctc ggcgatattg gcggaggctt
     1561 gctccggcgt gccgggcggc tcgcgccatt ctctcaaaat ctgggcggcc ttaggcatgg
     1621 tcttgtccag cggctcgctc accgcggttt ccgctgcctt gggcttgagc ggtgcgctcg
     1681 tttgacccag taaatcaccg tcttttctgg tgcgcaggcg tccgatgatg ttgtcgttca
     1741 agaccgcagc tccggagccc tgggccagac ccgtggaaat aatggatcga tcgctaatca
     1801 tgacttgcga agcctgttct acgtttcttc tgaaggtggc gttttgcttc atctcttttt
     1861 ctatggctgc ttgcctttcg gctccgctca gagtcttcag ggctttggcg ttgcccccca
     1921 ggtcttcaat ttgcttgttg ctgagagagc gagccagggc cgatctgtcc aggaagagcc
     1981 ggcgggcgcc ctccaccgac tcggccagag cgccgccgtg ataataggcc ccctcggtgg
     2041 tcttgagcac aatgcggccg tctttatgca actgcacgtc ggcggttggg gctgtctgag
     2101 cgcttttgcc gtaggtgccg tttttgattc tggctacggc atagcgcaat agctctcctc
     2161 tggccgccga gtccaactgt tctttcaggg tgggggaggc atgttcggtg gccttcatat
     2221 tggaataatt gcgggcccgt cccagggtgg cggccatgtc gtgatagagc gccttatctg
     2281 cttccatatc ggccagtttc gatttctgct cggcccagtc gatgagagca ttcaattgcc
     2341 tggtggcgtc tacgtcgctc gccctggtga ggctcggcag gggcggtcca tcggcaccga
     2401 gcctgagggc agagggactc tgcaccaatg agagaatctg ctgttccaat tccggtttga
     2461 agtccctggc ccgggccgct aaatctacat ctagatcgtc tactttcagc gccccggata
     2521 catcgaagat ttcgttgtcg atatggatga cgccatcctc tcgaatttta aagacgttct
     2581 tcttatcaga gttggctgtg gcatcaagga agtgcacatc accggttttc ttgttgacca
     2641 ggatgtagtc agcaccggct ctatccaggg gagagccggc ttcgctcggg taaatctgcc
     2701 agtcctggct aattttattc tgttgtttga gacggggcag gatgccgtct ttctcgctgg
     2761 tgagaaaatt ccagacagcc agttggctgc gttcacccat ggaatgagtc tggcgaaatt
     2821 caggtttgcg gatgatatcg gctttggtct cttccaggtg gcgtacggtc tggggcaatt
     2881 cctcctgtga ccagtgccga ctctctacta ctgccgtact ttccggcatt tccagtaagc
     2941 cggccttgcg cagggcctcc tgcgcttccg gcctggccgt gatcttattc ttgatttccg
     3001 ccggggtgtt gtcgaggaag tcttgatagg cctgggttcg ttccttggga ttggccttca
     3061 gccgctcacc gatttcgttg aaccagagct gagccgtctt ctccggcagg gtgccctctt
     3121 tacccctggc ggctagctcc ttaaataggg cctggcgaaa ttgcggctcg tcggctacat
     3181 ttttgagggc ataattgagg gcgctggcat cggccgagcc gaggttgtcg cctttcatca
     3241 aggcccttgt cacatagtag tcggcgctgg cggcgccagt gaagttcggg tccttgagcc
     3301 tgggttcgat gcttgccacg ataaactctt ccagttgctc ggcctggccc tggtcatact
     3361 tgcccagtgc ctgcctggaa gt
//
LOCUS       DAOQNZ010000265         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13837_length_3377_cov_3.816376, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000265 DAOQNZ010000000
VERSION     DAOQNZ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13837_length_3377_cov_3.816376"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PKW73_06050"
     CDS             <1..793
                     /locus_tag="PKW73_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA72877.1"
                     /translation="DLSYAGSNNPAQTLDLYLPTGGKPNKLLPLVVFIHGGAWLQGDK
                     ADSPGLIMAQHGFALASINYRLTNEAIFPAQIEDCRQAISYLRKNADKWGIDPSRIGV
                     WGVSAGGHLASLLGTSGSREKPEEQVQAVLNWCGLSDLMTVNEQSGSRTKIDYEDENG
                     PVAKFLGGRETKKPELAREASPVTYVSKDDPPFLIVHGDIDDVVPYAQSQELYEKLKK
                     AGVPCRLITIKGGGHMFGNEEEYKHAMQFFQSTIVQGRRDFVLED"
     gene            complement(804..1994)
                     /locus_tag="PKW73_06055"
     CDS             complement(804..1994)
                     /locus_tag="PKW73_06055"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNA72878.1"
                     /translation="MSVTLNTGETLVLPRSRTNSLFAVGLKCVKEGNYQKALQCFEAA
                     IAADKRNAYAYYYSALCLAKLGDRARSLAYLQLTSRNFAGSDAGRAAARTLKEIGTQN
                     ANYSALSSWSGRGNLPKETWIPFTVEGSSMVVDGIVNGVPVKMIFDTGAESCIFSLKH
                     LKQFGLSTPQGKPSTLVVGVGKDEPIPAWIVRVNLKVGRIEKYDFPVMVADMPVRHPL
                     LGQQFFNDFAYSVDNTAATISFKSRTEGHSDGKKPVQPAMTVNNSGKYVYSVPFRKDG
                     QALLVTCDVNGRKTEMVFDTGAELTLFSEKRATALALEVDRRRQVAIGGVAGATSANV
                     AVVRRLKLGPVERIDMPVLVTGKVSTRDVDGGEKGIALLGQDFFSGWHYKIDERLGTI
                     SFIR"
     gene            complement(2083..2376)
                     /locus_tag="PKW73_06060"
     CDS             complement(2083..2376)
                     /locus_tag="PKW73_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72879.1"
                     /translation="MKKQTLTEEGTAATQVDALERIARALELVVEKLAGLENKIEDLT
                     YEIQSHGSVIGDSLAHMVNEGEESLPFHMEADFEGGCGSGGCGSGGCGGSGSN"
     gene            2440..3372
                     /locus_tag="PKW73_06065"
     CDS             2440..3372
                     /locus_tag="PKW73_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72880.1"
                     /translation="MIEDKVGSAETRLQMILLKSQRPGKKLNRGRRMLVLLCFCLLVS
                     AAAVLKTAAKEPSKAPNSAPSGSNSLNKNASKLRVSADQCLRLKNYQEAERLYKEACE
                     AGDVRAATRLGAILEEGLSGKYDLDGAEKWYRLAARAGDPEAACRLAGILDYKQPQMA
                     KDDGSKPLTSTESLKWLKFAADGGYLDAQRRLATLYSRGKPSPERQALSFKYRMMAAK
                     QGDAKNNFKVGNYLINGIGCKKNAAAAVPFLKYAAETDPNACRLLASLYASGAPGLAQ
                     NQKEARLWQDRYRSLTKRNSQIDGEIIDSFNKKD"
BASE COUNT          847 a          961 c          883 g          686 t
ORIGIN      
        1 agatctgagc tacgccggca gcaataatcc cgctcagacc ctcgaccttt acctgcctac
       61 cggcggaaag cccaacaagc ttttaccgct ggtagtcttc attcacggcg gcgcctggtt
      121 gcagggcgac aaagccgaca gccccgggct aattatggcc cagcatggct tcgctctcgc
      181 cagcataaac tacagactga ccaatgaagc catatttccg gcacaaatag aagactgccg
      241 gcaagccata agctacctgc gcaaaaacgc cgacaagtgg ggcatagacc ccagccgtat
      301 aggagtctgg ggagtttccg ccggcggaca tctggcttct ttgctgggta ccagcggcag
      361 ccgggagaag ccggaggagc aagtacaggc agtcctgaat tggtgcggac tgagtgattt
      421 gatgacggtg aacgaacaat cgggctctcg cacaaaaatc gattacgaag atgaaaacgg
      481 tccggtagca aagtttctcg gcggacgtga gacaaagaaa cctgagttgg cccgagaagc
      541 cagcccggtt acctatgtca gcaaggatga tccgcccttt ctcattgtgc acggcgatat
      601 cgacgatgtg gtgccctacg cccagagcca ggaactctat gaaaagctca agaaagccgg
      661 agttccctgc cgtctgatta ccattaaggg cggcggtcac atgttcggca acgaagaaga
      721 atacaaacac gccatgcaat ttttccagag caccattgtg caaggccgcc gcgactttgt
      781 gctggaagac tgaagagttc gcttcagcga atgaaactga tcgtgcccag gcgttcgtct
      841 attttgtaat gccagccgga aaagaaatcc tgaccaagca gggcgatacc cttctcaccg
      901 ccgtcgacat cacgagttga gactttgcct gtgaccagca ctggcatgtc gattctctcc
      961 accggtccaa gtttgaggcg ccgcaccacg gcaacgttgg cgctggtggc cccggccacc
     1021 ccgccgatgg ccacctggcg ccggcgatcc acctccagag ccagagctgt agcccgcttc
     1081 tcactgaaga gggtcaattc agcaccggta tcaaaaacca tctcagtctt tcttccattc
     1141 acgtcacaag taaccaaaag cgcctgaccg tccttgcgaa aaggcacgga atagacgtat
     1201 ttccccgagt tattgacggt catggccggc tgcaccggct tcttgccgtc agagtgtcct
     1261 tccgtacggc ttttgaagga aatggtagcg gccgtattat cgacgctgta agcaaaatca
     1321 ttgaagaact gctgccctaa cagggggtga cgcacaggca tatccgccac catcaccggg
     1381 aagtcgtact tttcgatgcg ccccactttc aagttgaccc gcacaatcca ggccggtatg
     1441 ggctcatcct tgccgacgcc cacaaccagt gtggacggtt ttccctgggg cgtggacaaa
     1501 ccaaattgct tcaagtgctt gaggctgaag atacaggatt cggcgccggt gtcgaaaatc
     1561 atttttaccg gcacaccatt gacgatacca tccacaacca tggaactgcc ctctacagta
     1621 aagggaatcc aggtctcctt gggcaggttc ccacgaccac tccaactgga aagggccgaa
     1681 tagtttgcat tttgggtgcc gatctctttg agggttcgag cggcggctct accggcatca
     1741 ctgccggcga aattacggga cgtaagctgc agataggcga gactacgggc tctatctccc
     1801 agcttagcca gacagagggc actgtaataa taggcgtagg cattgcgctt atcggcggca
     1861 attgcagcct caaagcactg cagggctttt tgatagttac cctctttcac gcacttgaga
     1921 ccgacggcaa agagtgagtt agtgcgacta cgcggtaaaa ccagagtttc accagtgttc
     1981 agcgtgacac tcaaggaatt cggagactgc gcgcttgccg gcaaatagaa ggaagctgct
     2041 agtaacgctg cgaaatagaa ttggcgccgg gaaaacacag cattaattac tgccgctgcc
     2101 gccgcagcca ccgctcccgc atccgcccga gccacaacca ccttcaaaat cggcttccat
     2161 atgaaacggc aggctttcct ccccctcatt aaccatgtga gccagactgt cgccgataac
     2221 tgaaccgtgg gactgaatct cgtatgtcag atcttcgatt ttgttttcca gaccggcaag
     2281 cttctcgacc acaagttcaa gggcccgggc aatgcgctcc agtgcgtcaa cttgtgtcgc
     2341 ggctgtgcct tcttcggtca aggtctgttt tttcatttaa cgctccttca atcttgctcc
     2401 tattgtaaac caagtcgatg gcaatttcga atctatgaga tgattgaaga caaggtcggc
     2461 agcgcagaaa cccgtctcca gatgatctta ttgaaatctc aaagaccagg caagaagctc
     2521 aacaggggca ggcgaatgct tgtgctgctc tgcttctgcc tgcttgtaag cgctgcggcg
     2581 gtcctaaaga cagcggcaaa agagccgtca aaggcgccca attcggcgcc gtccggcagc
     2641 aatagtctta acaaaaacgc ttcaaagtta agggtaagcg ccgatcaatg cctccgcttg
     2701 aaaaactacc aggaagccga acgcctctac aaagaagctt gcgaagccgg tgacgtgcgg
     2761 gccgccaccc gcctgggcgc tattctggaa gaggggctga gcggtaagta cgatctagac
     2821 ggagcagaga aatggtaccg gcttgccgcc cgggcaggcg atccggaagc cgcctgccgg
     2881 ctggccggca tcctcgatta caagcagccg cagatggcaa aggatgacgg ctccaagcca
     2941 ttaacttcca cggagtcgct caagtggctg aagtttgccg cagacggcgg ctatctggat
     3001 gcacaacgca gattggcaac gctctattca agaggcaagc cctcgccgga acgccaggcc
     3061 ctctccttta aataccgcat gatggctgca aaacaaggag atgccaaaaa caacttcaaa
     3121 gtcggaaact atctcattaa cggcatcggc tgcaagaaaa atgctgccgc tgctgttcca
     3181 ttcctgaaat acgcagccga gactgatcca aatgcctgcc gcctgctggc ctcgctctat
     3241 gcaagcggcg cccccggtct tgcccaaaat caaaaggagg ctcgactctg gcaagatcgt
     3301 taccgcagcc tcactaaaag gaactctcag atagacggcg agataataga ttccttcaac
     3361 aagaaagact gaatgct
//
LOCUS       DAOQNZ010000266         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13843_length_3376_cov_4.175851, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000266 DAOQNZ010000000
VERSION     DAOQNZ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13843_length_3376_cov_4.175851"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKW73_06070"
     CDS             complement(<1..247)
                     /locus_tag="PKW73_06070"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNA72881.1"
                     /translation="MISGKAEERTRTEDTMHIGLGKKTRRAYGFDEIALVPGSKTVDM
                     ELCDISCSIGGIKFANPIIASAMDSVVDSHFAAEMGRL"
     gene            572..1777
                     /locus_tag="PKW73_06075"
     CDS             572..1777
                     /locus_tag="PKW73_06075"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNA72882.1"
                     /translation="MSALGHVLKSMNVLGLNSGTSMDAIDAGLFRIGPQLEGAIGGGL
                     CPPLKVEFLFSELVPFEPEFKGQLESFVARGESDLRTMCLLNSSLGEVFAAAALKVVR
                     QVDEKGLSVDLIGSHGQTIWHEPRPSQFWGLSAAGTLQLGDPAYVAARTGVTTVGDFR
                     TYDMAFGGQGAPLVSFADEVLFGHSGEALAVLNLGGIANITVLSPSGEAIMAFDTGPA
                     NVLIDEAMRILFGREFDEGGAVAGAGTVNEAVLSDLLKEEYFHLAPPKTTGRELFGRK
                     RASELVERLRAESIGPEDIVATLTALTARSIADAYKNFVASRVKITKLVLGGGGAENP
                     TLTSLLQKYWDGELTLYRHEDYGISTKFKEALLFALLAYTTYFSIPNNVPSCTGASRR
                     VCLGKIVKA"
     gene            1823..3070
                     /locus_tag="PKW73_06080"
     CDS             1823..3070
                     /locus_tag="PKW73_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNA72883.1"
                     /translation="MSGSESSSNSLIIPEGINYECMGCGKCCSGWSVPLTAPDYERIS
                     KVDWAAKNDKFEGMELFRELKDYEKKGSPYTHAIKHGDDGFCPFLVNNLCYIHSTFES
                     KTKPSICQLFPYNFNETPSGFVSTVSFVSVGAVKNSGKALTEQRDYLETKLGEFKALY
                     PDHHPNWSKIELVKEVPLTWNEYLEIEARLLAILSDGTKTLSLRLAHMSLAIVEVYLG
                     KRGSSLPESQKQIELNRHSLKKSDHVLLRELYRLYFPERLLNRADMNFSASKYMLSSF
                     ASKLGIGSPLSLKVPGDSYSIESVKEVTFPSGEVLEGILYRFIYQRIFGKLYFGAGYG
                     QLSLMVGFHHLVAAYVLLKLQARAVSRSRGFKAVQEADLISCVRMLEKRLGDSSLDGY
                     AAAVVELELQSHGRCLRLLSIAD"
BASE COUNT          720 a          832 c          923 g          901 t
ORIGIN      
        1 ccaggcgacc catttcggcg gcgaagtggc tgtctaccac ggagtccatg gcggaagcaa
       61 taattgggtt ggcaaacttg atgccgccta tgctgcagga gatgtcacag agttccatat
      121 ctactgtttt ggagccgggt accagcgcta tctcgtcgaa gccgtaggcg cggcgcgtct
      181 ttttgccaag tccgatatgc atggtgtcct ctgttctggt gcgctcttct gccttgcccg
      241 aaatcatagc ttgaataccc gcctttcagt ttaagtgatg tttccgcccg gactaagttg
      301 aaacccggtt gttgactgca tatcttagca ctgccatggg tccgaggtcg tggatgtcaa
      361 cgggcggatc tattcattcg cggctcgggg gcgggtgttt gtcgcccggc gcggcagttg
      421 gctggatttg aaaagtcgtt cacatttaga tatttgtgcg tacaattaag ctggatctaa
      481 cttaagcccc ctctccaatc aatgatgaga aaagaccggt ctgccgttaa attcacttac
      541 caccaatcct gacaggttag cgaggtagca gatgtccgcc ttaggtcatg tcctgaagag
      601 tatgaatgtg cttggactga acagtggcac ttccatggac gccatcgacg ccggtctctt
      661 tcgcatcggt ccgcagctgg aaggtgctat cggcggcggc ctgtgtcctc cccttaaggt
      721 cgagttcctc tttagtgaac ttgtgccgtt cgagcctgaa ttcaagggac agctggagag
      781 ttttgtagcc cggggcgaga gcgatctgcg taccatgtgt ttgcttaaca gcagtctggg
      841 ggaagttttc gccgctgccg ccctgaaggt cgtcaggcaa gtagacgaaa agggcttgtc
      901 cgtcgatctg atcggctccc atggacagac gatctggcat gagcctcgac cctctcagtt
      961 ctggggtttg tctgcggccg gcaccttgca attgggtgat cccgcttacg ttgccgctcg
     1021 caccggtgtc accactgttg gtgacttccg cacttacgat atggccttcg gcggtcaggg
     1081 cgcacccctg gtttcttttg ccgatgaggt gcttttcggc cacagcggcg aggccctggc
     1141 tgtgctcaat cttgggggga ttgccaatat taccgtactg tctccgagcg gtgaggccat
     1201 aatggccttc gacaccggtc cggctaatgt tttgatcgat gaggcgatgc gtatcctttt
     1261 cggccgggaa tttgacgaag ggggggcagt ggctggcgcc ggtaccgtca atgaagcggt
     1321 attatccgat ctcctcaaag aagagtattt tcacctggct cctcctaaaa caaccggaag
     1381 agagctattt ggtcgtaagc gcgccagcga gcttgtagag aggctccggg ccgaaagtat
     1441 tggaccggag gatatcgtcg ccacccttac ggctcttacg gcgcgttcaa ttgctgatgc
     1501 ttacaaaaat tttgtcgcat cccgggtgaa aattacgaaa ttggttctgg gcggcggtgg
     1561 ggccgagaat cccaccctca cttcgctttt gcaaaaatat tgggacggtg aactgacgct
     1621 ttatcgccat gaggattacg gcatttccac taagttcaag gaagccttgc tctttgcgct
     1681 tctggcttat actacttatt tttctattcc gaacaatgtg ccatcctgca caggagccag
     1741 tcgtagagtc tgtctcggca agatagtaaa agcttgagaa cggtcggcgt agttagagtt
     1801 atcggagaag atggagaaca atatgagtgg ctcagagtcg agttcgaact ccctgattat
     1861 tcccgaagga ataaattatg aatgtatggg ctgcggcaag tgttgcagtg gttggtctgt
     1921 accgctcact gctcccgact acgagcgaat ctctaaagta gattgggccg ccaagaatga
     1981 taaattcgag ggtatggagc tcttccgcga gttgaaggac tacgagaaga aaggcagtcc
     2041 ttacacccac gccatcaagc atggtgacga tggcttttgt ccgtttctcg tcaacaattt
     2101 gtgctacatc cacagcacct ttgagagtaa gactaagcct tccatatgcc agcttttccc
     2161 ttacaacttc aatgaaacgc cttcgggttt cgtttctacg gtcagttttg tcagtgtggg
     2221 ggcggtgaaa aattccggca aggccctgac tgaacagcgc gactacctgg agaccaagct
     2281 gggagagttc aaggctctct atcccgatca tcatcctaac tggagcaaga ttgagctggt
     2341 caaggaagta cctttgactt ggaatgagta cctggaaatc gaagccaggc ttctggcgat
     2401 tctttctgat gggaccaaaa ctcttagttt gaggttggcc cacatgtccc ttgccattgt
     2461 cgaagtctat ctaggaaagc ggggctccag cctgccggag agccagaagc agatcgagct
     2521 caatcggcat tcgcttaaga aaagcgatca tgtgcttttg cgtgaactct atcggcttta
     2581 ctttcctgag cgcttgttga accgagcgga catgaatttc tccgcctcca agtatatgct
     2641 ttcgtctttt gcatccaaac taggcatcgg ctcgcccctt tccctcaagg tccccggcga
     2701 ctcttattcg atagagtcgg tcaaggaagt aacttttcct tctggagaag ttctggaagg
     2761 aattctttat aggtttattt atcagcgcat tttcggcaag ctttatttcg gtgccggcta
     2821 tggtcagctc agtctgatgg ttggcttcca ccatctagtg gccgcctatg tccttttgaa
     2881 gcttcaggcc cgggccgtgt cccgtagccg cggattcaag gcggttcagg aagcggactt
     2941 gatcagttgc gtgcgcatgc ttgagaaacg gctgggggat agtagcctgg acggttatgc
     3001 cgctgcggtg gttgaattgg agcttcaaag tcatgggcgt tgtcttagac tcttatctat
     3061 agctgattaa gaaaacatgg ctttcaactt ggggagcaaa ttgtattgca ggcaacaaaa
     3121 acgctgttga cttaaggtcg gcatttgatt acattgtttg cacgtttcca agcggtacgc
     3181 cgcggagcga agttactaga aaaactggtt tgaaccgcag agcattagct ctgggtatcc
     3241 ggttagtgtt acctcaggag gtaattaact tatgcgtatg ctctccaaac ttactatggc
     3301 tttctctgct cttaccgttg caggcgctct ttcactcggc gctccggctc tagcccaaga
     3361 tgctttcggt tccggc
//
LOCUS       DAOQNZ010000267         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13898_length_3368_cov_3.008753, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000267 DAOQNZ010000000
VERSION     DAOQNZ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13898_length_3368_cov_3.008753"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(496..990)
                     /locus_tag="PKW73_06085"
     CDS             complement(496..990)
                     /locus_tag="PKW73_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72884.1"
                     /translation="MNNQSNLNQKTSASKPARARAGRGRKGQGLTEYAAMLAFAGIVA
                     AMAMGMVGNQAGALSSFGNACASQLDKVSSAGASAYSGSSGSSGTSSSSGSGTSSSSG
                     SGTSSGSGSGTPASSGSSGNGNSGNGNGNGNGNGNSGNNGNSGNGNSGNNGNRGNGNA
                     WGRG"
     gene            complement(1195..2685)
                     /gene="proS"
                     /locus_tag="PKW73_06090"
     CDS             complement(1195..2685)
                     /gene="proS"
                     /locus_tag="PKW73_06090"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNA72885.1"
                     /translation="MASDAKAQTEVKEPELDLDIDGDEGSVEGNSRWYTDTVTGAELA
                     DYAPVKGCMVIRPYGYALWENIKEALDGMIKETGHSNAYFPLFIPESFLKKEAEHVEG
                     FAPECAVVTHGGGKKLEEPLIVRPTSETIINYMFAKWVHSWRDLPLLINQWANVVRWE
                     MRTRLFLRTAEFLWQEGHTAHATEEEAEAEAKTMLECYRKLAEDWLALPVLTGEKTER
                     ERFAGAVRTYCIEAMMRDGKALQAGTSHFLGQNFAKAFDIKFQSQAGQLEYAWQTSWG
                     VSTRLIGAVVLGHGDEKGLILPPRIAPIQTVVVPIYKTDAEKETVLSSCRELETALKA
                     AGIRVHVDDRDNHTPGFKFNHWELRGVPLRINIGPRDVAGNVVEIARRDNQEKMRGVA
                     REGLPATIKDLLETVQKAIFERALKFRRDNTVKATTYEEMKAILKDKSVFVETHWDGT
                     SEDEGKIKEECKATIRCLPFAFEPVEGTCMYTGRKTTRRVIFAKAY"
     gene            2840..>3368
                     /locus_tag="PKW73_06095"
     CDS             2840..>3368
                     /locus_tag="PKW73_06095"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA72886.1"
                     /translation="MQLSGELAKVSFANLLQLVRSGGLAGKLTLSQGARLGAILIEKG
                     LPVHAEIEGLSGKEALFEFFLWLSGTFAFSEGAFEGLNKTLPRTIKFNNSDDTFERLL
                     KDGIAYLEAKQYLDSLGVGPESVLKAADQKGDYARTLMQNPGLERIDGVRTLADALSA
                     LNLTGREFTLVVADWF"
BASE COUNT          625 a          914 c          975 g          854 t
ORIGIN      
        1 ggaagccccg acggcaaagt cgggggcgcc ctcacgcgcc tgcgatttag aggttttggt
       61 catatgacac tccatagaga cgtttagatg tgcggctgcc gtcagagccc cgtagaggcc
      121 tctggctgag ctggaacatt gagcttcgag agctcagctt ggttttcttt tcacgagcgt
      181 cgatagcttt catgccgccg gtatagcgat gagttgcttc catcaccggc tgaggtgact
      241 gaggaaggct cgggggcggc agtgggggtg ttgcagctat ggatgggggg tgaaataagg
      301 aaacaaaaga gatgccagga gtctagaaac tcctggcatt accggcctac tttctttggg
      361 ttagattgag atctaaatgg tcctcctttg cttttatgtt cctcatcttg ccgtagggtg
      421 gggcttgtag ttaatggccc ggctcgcgct agagcgcctc tttgaaactt gcggtgtgtc
      481 tatctttctt ctggattaac cgcgacccca ggcattgccg ttgcctctgt tgccgttgtt
      541 tccgctattg ccgttgccgc tgttgccatt gtttccgcta ttgccattgc cattgccatt
      601 gccattgcca ttgccgctgt tgccgttacc gctggaaccg ctgctggcgg gggtgccact
      661 gccgctgccg gaggaagtac cgctgccgct gctggaggaa gtaccgctgc cgctgctgga
      721 agaagtaccg ctgctaccgg aagaaccgct gtaggcgctg gccccagccg aggacacttt
      781 gtcgagctga ctggcacagg catttccaaa gctggagagc gcaccggctt gattgccgac
      841 cattcccatg gccattgctg caacaattcc ggcgaaggcc aacatggctg catattcagt
      901 caggccctga ccctttctgc cccgtccggc gcgggctctg gcgggcttgc tggcggaagt
      961 tttttggttg aggttagact gattgttcat gatgtttatc tctcatgtgt agcgatttag
     1021 ttggcgggac tcgctgcccg gtgatttcat cttacgtccg gccggggtgc tgaaacatcg
     1081 gcgcattgcc cggttctcct atcggtcaag gtaccgatat ttgtctttga gagaattgat
     1141 ggcaaaagaa aaccccctgc cggggcaggg ggcgtccata tcaaaagcct ttcattagta
     1201 ggcttttgcg aagatcaccc ggcgggtagt tttgcgaccg gtgtacatac aagtaccttc
     1261 caccggctca aaggcgaaag gcaggcaacg aatggtagcc ttgcactctt ccttgatctt
     1321 gccttcgtct tcgctggtgc cgtcccaatg ggtttcaacg aaaacgctct tgtctttgag
     1381 gatggctttc atctcttcgt aggttgtagc tttcaccgtg ttgtcccggc ggaacttgag
     1441 ggctctttcg aagatggctt tctgcactgt ttcgagcaga tccttgatgg tggcaggcag
     1501 tccctctctg gctactcccc tcattttttc ctggttgtcc cggcgggcaa tttcaactac
     1561 gttgccggcc acgtcgcggg gaccgatgtt gattctcaga ggcacgccac gcagctccca
     1621 gtggttgaat ttgaagccgg gggtatgatt gtcccggtca tccacatgga cacggattcc
     1681 agcggctttg agagccgtct ccagttcccg gcagctggag agtactgttt ctttctcagc
     1741 gtcggtcttg tagatgggta ctaccacggt ctggatgggg gcgatgcggg gcggtagaat
     1801 caagcccttt tcgtctccat gacctaagac cacggctccg atcaagcgag tggaaacccc
     1861 ccaacttgtc tgccaggcat actccagttg accggcctga ctctggaatt tgatatcgaa
     1921 ggccttagcg aaattctgac cgaggaagtg cgaagtgcct gcctgtaggg ccttgccgtc
     1981 gcgcatcatg gcttcgatgc agtaggtgcg cacggcgccg gcgaaacgtt cccgttcggt
     2041 cttctctccg gtgagaaccg gcagagccag ccaatcttcc gccagcttgc gatagcactc
     2101 cagcatggtt ttggcttccg cttccgcttc ctcttccgtg gcgtgggcgg tgtgcccttc
     2161 ctgccagaga aactcagcgg tgcgcaggaa gaggcgggtg cgcatttccc agcgcactac
     2221 gttagcccac tggttgatga gcaggggcag gtcgcgccag gaatgaaccc atttggcgaa
     2281 catgtagttg atgatggttt ccgaggtggg gcggacaatg agcggctctt ccagtttctt
     2341 gccgccgcca tgggtgacca cggcgcactc gggagcgaag ccctccacgt gctcggcttc
     2401 tttcttgagg aaggactccg gtatgaataa aggaaagtag gcgttggaat ggcctgtctc
     2461 tttgatcatg ccgtccaggg cttccttaat gttttcccag agagcgtagc cgtaagggcg
     2521 tatgaccata caacccttga ccggggcata gtctgccagt tccgctcccg tgacggtgtc
     2581 ggtgtaccag cgggaattgc cttccacgct gccctcatcg ccgtctatgt ccaggtccag
     2641 ttccggctcc ttgacttcag tttgcgcctt tgcatcagag gccatttgct ttatctccct
     2701 tgaggttttg acagtataga ccaagccgct ccgccctgaa gccatcgccg ggcagtgttt
     2761 gcctagtgtt aaggctcgcg gctataattt acgaagcagg ggctgagaaa ttcagccgtt
     2821 ttaaataatt cggagtccta tgcagctttc cggcgaatta gccaaagtta gttttgccaa
     2881 tcttttgcag cttgtcagga gtggcgggct ggcaggcaag ctcaccctct cgcaaggggc
     2941 gcgcttgggc gccatcctga ttgaaaaagg gctgcctgtt catgccgaga tcgagggact
     3001 atcaggaaag gaagcactct ttgaattctt cctctggttg agcggcacct ttgccttctc
     3061 cgaaggcgcc ttcgagggtc tcaacaagac gctgcccaga acaatcaagt ttaataactc
     3121 cgacgacact tttgagcgtc ttctcaaaga cggcattgcc tatctggaag ccaaacagta
     3181 tcttgattct ctcggtgtgg gtccggaatc ggttttgaag gcggcggacc agaaaggtga
     3241 ctatgcccgc accctcatgc agaatcccgg gctggagcgc atcgacggtg ttcgcaccct
     3301 ggctgatgcc ctgtcggccc tgaatctgac cggtcgagaa tttaccctgg tagtggccga
     3361 ctggttct
//
LOCUS       DAOQNZ010000268         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13903_length_3367_cov_2.985507, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000268 DAOQNZ010000000
VERSION     DAOQNZ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13903_length_3367_cov_2.985507"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PKW73_06100"
     CDS             <1..849
                     /locus_tag="PKW73_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72887.1"
                     /translation="GISKSQKILLEELWSTAYHEAGHTIVTYFKGKKELIQVVTIVPT
                     GRALGFMWAVRKDDPSHHAPTREELLTEIEISLGGYVAESFYMETTTAGVSQDLVNVA
                     QTASAMVRNYGMGSFVFNTDRSLGDRINAGGRYGKASEETEAQIDREIKKIVDDCVES
                     TRNLIKSKRAELDKIARALVEKETLYYRDLVAILEPTRSQEDIEREINALAERKQVGT
                     PAVISLDLLPGLTGISTGNGKNGKKNGNGSHSDSSDKPDATGAAGTSQDKPADGKDGE
                     NQPQQN"
     gene            821..1975
                     /locus_tag="PKW73_06105"
     CDS             821..1975
                     /locus_tag="PKW73_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HNA72888.1"
                     /translation="MARISLSKTKSLKRGFGARAIAMAVALAASVPVLAQAPPGAPQK
                     PKAPPEVLSCRPDPSLQRRIDSKVGFCGGDAAYSIPLSNGSTVWLFGDSFRGKIVGGK
                     RTGCRMERNCVAVDKPRQAGGKLQYCFGKRDFFICREEDGTNYYWPGDGIEIDGRLYL
                     FQHTVKTDRQLPAPFQFKMRDNHIIVVLNPKDSPDKWVQRVFRLKQSAGIALLSVACY
                     QEGDYLYMFNSYTGMAIGTVRNPTALSRIKLSDLAQVNMKEMEWWSGKWVKARELPEI
                     LFEDGASEMSVVRIEGLKGLYCFYIPSDRSAIMVRTAMRPEGPWSPRKEVYKFPKDPE
                     LFYYSVKAHPEYKAGPGKIKLTYNVNSLHLKRLMEDDQVYFPKALEVTLK"
     gene            complement(2031..2660)
                     /locus_tag="PKW73_06110"
     CDS             complement(2031..2660)
                     /locus_tag="PKW73_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72889.1"
                     /translation="MKPERSQFTRTELRIIDQYRTPYQVQRYLKTLSYNHDHTIWSFR
                     EVVKRKTAHCLEGALTAAAILEQHGYPPLLMSLESVDNLDHVLFVFRASTGWGAVGRS
                     RDTGLHGRRPVFRSARDLALSYFDPYVDNSGRITAYGVVNLNDLSRCNWRFSESKLTR
                     VENYLIECDHTDIVSSERRYQRLLKRFKEFKAKYPNRQVTYFDNKDTWM"
     gene            complement(2832..>3367)
                     /locus_tag="PKW73_06115"
     CDS             complement(2832..>3367)
                     /locus_tag="PKW73_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72890.1"
                     /translation="TWTFYMCSVSPNIILWASDRRYLHELLARRKSKIKRQAFLSSSE
                     LWRNLDVRSDFFALRDFRKCKSPTDSLSTFFLLEPSPERLYIDTGLVGYRVSYKYSTT
                     TLEETYISYDWKAQEIREYHETHSKSFRDELVPCKMAYRRGTWLNVSTDVSKGSSDAH
                     FIFLFGALSLLGHTLVV"
BASE COUNT          928 a          909 c          841 g          689 t
ORIGIN      
        1 ggcatttcca aaagccaaaa aatcttgctg gaagaactct ggagcacagc ctatcacgaa
       61 gctggacata caatcgtcac ttacttcaaa ggcaagaaag agctgatcca ggtggtgacc
      121 attgttccca ctggcagagc cctgggcttc atgtgggcgg tgcgcaaaga cgatccctcc
      181 caccatgccc ccacccgcga agaactgctc accgaaatcg aaatcagctt gggcggctat
      241 gtcgccgaaa gcttctatat ggagaccacc accgcgggcg tctcacaaga ccttgtcaat
      301 gtggcgcaaa cggcatcagc catggtgcgc aattatggta tgggatcttt tgttttcaac
      361 accgatcgca gcctcggcga cagaattaac gccggcggcc gctacggcaa agcctcagaa
      421 gaaacggaag ctcagattga tcgcgaaatc aagaaaatag tcgacgactg cgtggagagt
      481 acccgcaacc tgatcaaatc aaaacgggct gaactggaca aaattgccag agctctggtt
      541 gagaaagaga ccctctacta ccgcgacctg gtggccattc tcgaacccac tcgcagccag
      601 gaagacattg aaagagaaat caatgctcta gccgagcgca agcaggtggg cacgccggct
      661 gtcattagcc tcgaccttct acctggactg actggtatta gcaccggcaa tggcaagaac
      721 ggcaagaaaa acggcaacgg cagccactct gattcgtcag ataaacctga cgccacaggc
      781 gcagccggaa ccagccagga caagccagcc gacggtaaag atggcgagaa tcagcctcag
      841 caaaactaag agtctgaaaa gaggcttcgg tgcaagggca attgccatgg cagttgccct
      901 tgccgctagc gtgccggtgc tagcccaggc tccgcccggg gcaccacaaa agccgaaagc
      961 cccgcctgag gtgctgagtt gccgccccga cccatccttg caaaggcgca tcgatagtaa
     1021 ggtcggtttc tgcggcggcg acgccgccta ttccatacca ctttcaaatg gaagcacagt
     1081 ctggctcttc ggagacagct ttcgcggcaa gattgtcggg gggaagcgca ccggctgccg
     1141 catggagcgc aactgtgtag cagtggataa gccacgacaa gctggcggca aactccagta
     1201 ttgtttcggc aaacgcgact tttttatttg ccgggaagaa gacggcacca actactactg
     1261 gcccggcgac ggcattgaaa tcgacggacg cctctatctc tttcagcata cggtcaagac
     1321 cgaccgacag ttgccagcac cctttcagtt caagatgcgc gacaatcaca taatcgtcgt
     1381 actaaacccc aaagacagcc ccgacaagtg ggtccagcgc gtcttcagat tgaagcaaag
     1441 cgccggcatc gccttactct cagtggcttg ctaccaagaa ggcgattatc tctatatgtt
     1501 caactcctac acaggcatgg ctattggcac agtgcgaaat cccacggcgc tttcccgcat
     1561 caaactgagc gacctcgctc aggtcaacat gaaagaaatg gagtggtggt cgggcaaatg
     1621 ggtgaaagcc agagagttgc ctgagattct gtttgaagac ggggcttcgg aaatgtccgt
     1681 tgtaagaatt gagggcttaa agggtctcta ctgcttctat attccttcag acaggtccgc
     1741 tatcatggtg cgcacggcca tgcgcccgga aggaccctgg agcccccgca aagaggtcta
     1801 caagtttccc aaagaccccg aacttttcta ctacagcgtc aaagcccacc ccgaatacaa
     1861 agccggaccg ggcaagatca agctcaccta taacgtcaac agcctgcatc tcaagcgcct
     1921 catggaagat gatcaggttt acttcccgaa ggcgctggaa gtaaccctca agtaagtccc
     1981 gactgcatca atgaagcata tatcagcgct gggctatagt tccggcagtc ttacatccag
     2041 gtatctttat tatcgaagta cgtcacctgt cgattgggat acttggcctt gaactccttg
     2101 aaacgcttca gtaggcgctg atagcgccgc tccgacgaaa caatgtcggt atggtcgcat
     2161 tcaatgagat agttctccac cctagtcagc ttagactccg aaaatctcca attacagcgg
     2221 ctgagatcat tcaaattgac gacaccatag gcggtgatac gtccgctatt atcgacatag
     2281 gggtcaaaat agctgagagc aagatctcgt gcgctgcgaa aaacgggacg gcgcccgtgc
     2341 aagccggtat cgcgcgagcg accgactgcc ccccagccgg tggaagcacg aaagacgaac
     2401 aaaacgtggt cgagattatc tactgattca agactcatga gcaaaggcgg atagccgtgt
     2461 tgctccagaa tggcagccgc agtcaacgcc ccttccaggc aatgagccgt ctttcgcttt
     2521 accacttcgc gaaaggacca tatcgtatgg tcatgattgt aggaaagcgt tttcagatat
     2581 cgctgcacct ggtagggcgt gcggtactgg tcgattatcc tcaattcggt gcgggtaaat
     2641 tggctgcgct ctggtttcat gctgccatta taaacaaatg agacccatca gcccacaagg
     2701 gcaagcaaaa gagtaatcta tcaagtaagg cttggttatt ttcaaactca cgaaccttag
     2761 gcaatcactg gcgctagtaa ctccaacccg gcagaaacag caaactcgct gtccaagcag
     2821 caccaaccga ctcaaacaac caaggtgtgc cctaaaagag aaagggcacc aaatagaaaa
     2881 ataaagtgag catcactcga ccccttcgat acatcagtag aaacattcaa ccatgtcccc
     2941 ctacggtaag ccattttaca gggaacaagt tcatcgcgga aagactttga atgagtttca
     3001 tggtactctc gtatctcctg cgctttccaa tcataagaaa tgtaggtctc ttcaagagta
     3061 gtcgtactgt acttgtatga aaccctgtag ccaaccagtc ccgtatcaat gtatagacgc
     3121 tcaggggagg gctcaaggag aaaaaacgtg cttaaggaat ctgttggact tttgcacttt
     3181 ctaaaatctc tcagggcaaa gaagtcagaa cgaacatcta aatttctcca aagctcactg
     3241 gatgaaagaa aagcctggcg tttgattttc gacttgcggc gagccaaaag ctcatgtaga
     3301 tagcgcctgt cgctagccca aaggataata ttgggcgaca ccgagcacat atagaacgtc
     3361 catgtgt
//
LOCUS       DAOQNZ010000269         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_13950_length_3362_cov_2.504385, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000269 DAOQNZ010000000
VERSION     DAOQNZ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13950_length_3362_cov_2.504385"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKW73_06120"
     CDS             complement(<1..481)
                     /locus_tag="PKW73_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNA72891.1"
                     /translation="MNSAKTEQALKIEDFEYDLPPELIAQKPLDKKDQSRLFVVDRGR
                     EAFAHKHFFELPTLLKSGDLLVVNDTRVLPSRLVAQRHSGGTIKILLLKPLPTNTAIW
                     ESMVTPIKRLKPGEKLTVKRDDGIESEITVHDIVNGEDGFKRLLVDLGAKEAVYELLS
                     "
     gene            complement(462..1337)
                     /locus_tag="PKW73_06125"
     CDS             complement(462..1337)
                     /locus_tag="PKW73_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72892.1"
                     /translation="MKAQLQKSATLLLTLLMLSLPLPALSKAYFAGRKEMVEKADLIA
                     LVDVAAVEKCERKGEPFSYSQKAPARVLQVYKGKCGDNLTIYGGENFICARLNIEPGK
                     ALAFLRKEKEEGAYTGSNWHLSLLPVTANGKQVKWFKAPQERELSELSLAAAKQDIKD
                     DLECRTILEQSPAFIKTIYGAQRLAGSAVGEGAEKSPLYLAYLEALKNAAKYKKELTM
                     TARYGSPAGRLYAVSALAAGQAEAARKLLTQMSNCKDNVYYQSGCKGTNELLGTISQE
                     LVKKGRYLDFELGKD"
     gene            1416..2570
                     /gene="queG"
                     /locus_tag="PKW73_06130"
     CDS             1416..2570
                     /gene="queG"
                     /locus_tag="PKW73_06130"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNA72893.1"
                     /translation="MKERIRELALSLGFNRLVVASPAPMPEALDTYSAWLERGFSADM
                     DYLKRDPQRRVTPAMTFAPARSILIVSVNYYNSPPPPPENMVWGRVAGYAVGLDYHNV
                     LRAKLRDLFALIEKEVGHPLSRRAVTDDAALFEQALAARHGLGFVGKHTLIIGPKLSG
                     SYNLIGELFLDLELEPDEPYVGTCGACFRCGTACPTGAIVPATEGSGYVVDANACLSY
                     LTIENKGGIAPAMRAGLGDWVFGCDICQDVCPYNSKPVASPWPEFKPERGAGHYLDLL
                     GLFDAVSEEEFRARFLNSPLRRPKLRGLRRNALVVLGNKLRGGKVELAYLRHRLVAYL
                     EREEDEMLLEHAAWALCQGRDKPVALVERFLPRIKSTESRQAVVGYLEGE"
     gene            2606..2980
                     /gene="crcB"
                     /locus_tag="PKW73_06135"
     CDS             2606..2980
                     /gene="crcB"
                     /locus_tag="PKW73_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNA72894.1"
                     /translation="MQFLIVFLGAGLGGVCRYLSSTTLQKLLPTLSFPVGTYFVNMLG
                     CLLIGMIAQLAETRIALQGDTRLFLTVGFLGGFTTFSSFGYETVQLIKDGEFLLAAFN
                     AVSQVVLGLLFVWLGMVLAKLV"
     gene            3027..3362
                     /locus_tag="PKW73_06140"
     CDS             3027..3362
                     /locus_tag="PKW73_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNA72895.1"
                     /translation="MATTGKGLLLKVFVGETDRKGKIPMYEWLVKEAKSYGIAGATVF
                     RGLMGFGSHSTLHTAKILDLSTNLPMVIEFMDTDEKIRGFLAVIEENVQDGMATLQEI
                     EMHVYSSKK"
BASE COUNT          659 a          862 c          933 g          908 t
ORIGIN      
        1 ggctcagtaa ttcataaact gcctcttttg caccaaggtc taccagtagt ctcttaaagc
       61 cgtcctcacc gttcactatg tcatgcacgg taatttcact ttcaataccg tcatcgcgtt
      121 ttacggtcaa tttttcgccg ggcttaaggc gcttgatggg cgttaccatg ctttcccaga
      181 tggcggtatt ggtgggcaga ggcttgagca aaagtatttt gatggtgccg cctgaatgtc
      241 tctgagctac cagcctggaa ggcagtacgc gggtgtcgtt caccaccagc agatcgccgc
      301 ttttgagcaa ggtcggcagt tcgaaaaaat gcttgtgggc aaaggcttcc cgccctctat
      361 ccacaacaaa aagccttgac tggtctttct tatccagggg cttctgggcg ataagctcgg
      421 gcggcaggtc gtattcgaag tcttctatct tcaaggcttg ttcagtcttt gccgagttca
      481 aaatctagat accgtccctt tttcaccagc tcctggctaa ttgtaccaag cagctcattt
      541 gtccctttgc agccgctttg atagtaaaca ttatccttac agttgctcat ctgcgtgagg
      601 agcttgcggg ccgcctcggc ctgaccggcg gccagggccg agaccgcata aagcctgccg
      661 gccggcgagc cgtagcgtgc cgtcatggtc agttctttct tgtacttggc ggcatttttc
      721 agggcttcca gataagccag gtagagcggc gatttttcag ccccttcacc gacggcgcta
      781 ccggccagcc gctgtgcccc gtaaatggtt ttgataaaag ccggggactg ctcaagaatg
      841 gtccggcatt ccaggtcgtc ttttatatct tgcttagcgg cagccagact aagctctgag
      901 agttctcttt cttgcggtgc tttgaaccac ttcacttgct taccgtttgc agtcactggt
      961 aagagcgaaa gatgccagtt ggagcctgtg taggcacctt cttctttctc ttttctgaga
     1021 aaggccaggg ctttgcccgg ttcgatgttg agcctggcgc aaatgaagtt ttcgccacca
     1081 tatatggtta gattgtcgcc gcacttccct ttataaactt gcagcacccg ggccggggcc
     1141 ttttgtgaat aactgaaagg ttcgcctttg cgctcgcatt tttccacggc cgctacatct
     1201 accagtgcaa taaggtcggc tttttccacc atttctttgc gaccggcgaa gtaagctttg
     1261 ctgagcgccg gcaggggcag gcttagcata agcagggtga gaaggagtgt ggctgacttt
     1321 tggagctgtg ctttcatttt gcatttcctc tgccacatta taaaacgcct tccggctttg
     1381 ctactattta agcaacattt tggggctgac gctgattgaa ggaaagaatt agagaactgg
     1441 ccctctccct gggtttcaac cgcctggtgg tggcatcacc ggcgcctatg cccgaggcgc
     1501 tggatactta cagtgcctgg ctggagcgcg gcttcagtgc cgatatggat tatctgaagc
     1561 gagacccgca aaggcgagtc acacccgcaa tgacctttgc ccccgcccgt tcgattctta
     1621 tagtatccgt caattattac aacagcccgc caccgccgcc cgagaacatg gtctgggggc
     1681 gtgtggccgg ttatgcagtg gggcttgatt atcacaatgt gttgcgggcc aagctgcgag
     1741 atctattcgc cctcatcgaa aaggaagtgg ggcatcccct ttcccggcgc gccgtcaccg
     1801 atgacgccgc cctcttcgag caagcgctgg ccgcccggca tgggcttggt tttgtgggca
     1861 agcacaccct catcatcggc cccaagctgt cgggcagcta taacctcata ggcgaactct
     1921 tccttgatct cgaactggag ccggacgaac cctatgtcgg cacctgcggt gcttgctttc
     1981 gctgtggcac ggcctgtccc accggtgcca tagtgcctgc caccgagggt agtggctatg
     2041 tggtggacgc caatgcctgc ctctcctacc tgacgattga gaataagggc gggattgctc
     2101 cggcaatgcg cgccggtctg ggcgattggg tctttggctg tgatatctgc caggatgtct
     2161 gcccctataa cagtaagcca gttgcctcac cctggccgga attcaaaccg gagcgcgggg
     2221 ccggccatta tcttgatctg ttaggacttt tcgatgctgt ttccgaagag gagtttcggg
     2281 cgcgctttct taattcgccc ttgcgtcgcc ccaagctgag aggcttacgt cgcaatgctc
     2341 tggtggttct cggcaataaa ctgaggggcg gcaaggtaga gcttgcttat cttcgccaca
     2401 ggcttgttgc ttatctggag cgcgaggagg atgaaatgtt gcttgagcat gcggcctggg
     2461 ccctctgtca gggccgtgat aagccagtgg ctctggtgga gcgctttctg ccccgcatca
     2521 agagcactga gtcgaggcag gctgtggttg gttatttaga gggtgaatga agctataata
     2581 gcggcagttt aatgcggatt gtgccatgca gtttttgatt gtctttcttg gagcgggtct
     2641 tggtggtgtc tgtcgctacc tgtcatcaac taccttgcag aagcttctgc ccaccttgag
     2701 ttttcccgtg ggcacctatt ttgtaaatat gttgggttgc cttttaattg gcatgattgc
     2761 ccagttggct gaaacaagaa ttgccctgca aggcgacacc cgcttatttc ttacggtggg
     2821 cttcctgggc ggttttacaa ctttttccag cttcggttat gagaccgtgc aactcattaa
     2881 agacggggag tttctgctgg cggccttcaa tgctgtttcg caagtggtgc tgggtttact
     2941 cttcgtctgg ttgggcatgg tgctggcgaa actcgtttag tttaatttag tttggcttag
     3001 tttggttttg caatgggggg acgccggtgg caacaacagg taaggggctt ttactgaagg
     3061 tctttgtcgg tgaaaccgac cgcaagggca agatacccat gtatgaatgg ctggtcaaag
     3121 aggcgaagag ctatggtatc gccggtgcta ccgtgtttcg cggtctgatg ggttttggtt
     3181 cgcactccac cttgcatacg gctaaaattc tcgacctgtc caccaattta cccatggtga
     3241 ttgagttcat ggatacggat gagaagattc gcggctttct agctgtgatt gaagagaacg
     3301 tgcaagatgg catggctacc ctgcaagaaa tcgagatgca cgtatatagc agcaaaaagt
     3361 ag
//
LOCUS       DAOQNZ010000270         3332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14164_length_3332_cov_3.074153, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000270 DAOQNZ010000000
VERSION     DAOQNZ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14164_length_3332_cov_3.074153"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1293)
                     /locus_tag="PKW73_06145"
     CDS             complement(250..1293)
                     /locus_tag="PKW73_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72896.1"
                     /translation="MTPTTLEKNQLDFLNETVKAPAFSFFFRLHSGSEKQLHDTGHIR
                     SRCHEVFKASAVDAEVKERLMRECDALIEALDWQSGAKSLGLYVADGAAFARMFHVHL
                     PEVYYMGDNFSVYETLYAFEACAPYLLLLFTPGGLEIYRGQGSYLETLPKTKEVVHLL
                     SVYAHRATRHADKDGKGRKGDEIDHKWKDDLFRAWQDVAAAEGLPMYAAGLEQIGAGA
                     EEVKARGLNLFYGSESTWTRTSPEELKPLVKDLRNEYRKYLAAQYVTRIEAAFGAHKM
                     VSSLDEIVDCARAGKAEVLLLEDPQWTTEVEAKFTRLHTAIAETLSRGGRVEFLPEGS
                     LSKWQGAALILRY"
     gene            complement(1462..2523)
                     /locus_tag="PKW73_06150"
     CDS             complement(1462..2523)
                     /locus_tag="PKW73_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72897.1"
                     /translation="MPKNAAVISREILDSKEVLLGVYMNDVRDFILVSNFGLHWIYGE
                     VSRFVPYSSLKSFELPQELEEGTIDLLLKDGSALTIPVCNETEGSPDLLSFQLYLQAV
                     IDYLEPPEEALIRQIAGPEQLASYLLSAGGPFARLYGEPLARALTSRADLAEKFMSFG
                     IDPGLLKQEQFFRAIALLTSLRSRDSLEVELVRQWYLLDQKGGDSRFSDGLSEKEELG
                     FPEQKVMDCLDRECLRVFSLALEEVESMGGTCLGAEFLLLALVRNHFNEAQAVFGVES
                     LSLDSLRLAVRRERGPAPGTPAGTLPFSAQTVSILDAAFEEAHASGDGTIRPLNLLSQ
                     FHRLETGVAASVLRQINRV"
     gene            complement(2647..>3332)
                     /locus_tag="PKW73_06155"
     CDS             complement(2647..>3332)
                     /locus_tag="PKW73_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA72898.1"
                     /translation="AMAKVLLVEDDLVLAHSIQLWLKGEKHVVEHVAEGNQALELITF
                     SSYEVIILDWELPGLSGIDICRRLRMAQNKVPILMLTGRDSEEHKVTGLDCGADDYLT
                     KPFSLKELSARIRAIVRRSAGVACNVLQAGDIVLDAESHRVTRGGEDIELMPREFAVL
                     EFLMRNKNQVFSSEALLQKLWHTDSDSSREAVRACIKRLRQRIDQSEDEGSSFIETVP
                     KIGYRIRRG"
BASE COUNT          851 a          963 c          791 g          727 t
ORIGIN      
        1 agatcgagag cctgggctat ggcgattttc gaacaattgg agccgtcaat ggcaaagaga
       61 atcttcataa aagtgaaccc gagagggaaa tacaaattca gcagctcaaa tttagccact
      121 tgtctgaaga agttcaatca ccctaaagac gggtttaatc gtaaccttag tcatcagagc
      181 agctacttta ccttgcagaa aggggaggct tgcgcctccc ctccttaacg aacggctctc
      241 tgcccggcgt tagtagcgca gaatcaaagc ggcaccctgc cacttgctca aactgccctc
      301 aggcagaaat tcaacccgac cgccccggga gagggtctcg gcaatagccg tatgcaacct
      361 ggtgaattta gcctctactt ccgttgtcca ctgcgggtct tccagaagta gaacttcagc
      421 ttttccggct ctggcacagt cgacaatctc atcgagactg gagaccattt tgtgagcacc
      481 aaaggcggct tcaatacgag tgacatattg agcagccaga tacttgcggt attcgttgcg
      541 caaatccttg accagaggct tgagttcctc aggactggtc ctggtccagg tgctctcgct
      601 gccgtaaaac agattcaggc ctctggcttt cacttcctca gctccggcac cgatttgctc
      661 cagaccggct gcatacatgg gcagcccctc ggcggcagcc acatcctgcc aggcgcggaa
      721 gagatcatct ttccacttgt gatctatttc atcacctttg cgacccttgc cgtctttatc
      781 tgcatgcctg gtggcgcggt gggcataaac cgacaagaga tgcactactt ccttggtttt
      841 gggcaaggtt tccagataac ttccctgacc cctgtagatt tcaaggccgc ccggcgtgaa
      901 tagcaagagc aggtaggggg cgcaagcctc aaaggcgtag agggtttcgt agacggagaa
      961 gttatcaccc atgtaataaa cttccggcag gtgcacatgg aacatacgag caaaggctgc
     1021 gccgtcggca acatataaac ccagagactt agcaccgctc tgccaatcaa gagcctcgat
     1081 caaggcgtcg cattcgcgca tcaggcgctc tttcacctcg gcatcaacag cgctcgcctt
     1141 aaacacttca tgacaacgac tgcgaatatg accggtatca tgcaactgtt tctcagaacc
     1201 ggaatgaaga cgaaagaaga agctaaaagc aggcgctttc actgtctcgt tcaggaaatc
     1261 caattgattc ttttctagtg tcgtcggtgt catttgcata cctacttaat ttgatttcaa
     1321 gatgtaattt aagggttttc caacgcgcta attctagatc cctccatgag ggaatttatc
     1381 cgccacatgg cttactgaca ggatgagaac cggcgtctgc ctaccgcagg ggtcaatctt
     1441 tcgactgacg ctgcccgtac cctaaactcg attgatctgc ctgagcaccg acgccgccac
     1501 accggtctcc agacgatgaa actgcgatag caaattcaag ggtctgattg tgccgtcacc
     1561 actggcatga gcttcctcga atgccgcgtc caaaatactg acggtctgtg cgctgaaagg
     1621 caaagtaccg gctggagtgc ctggcgcggg accgcgttcc cgcctgacag ccaggcgcag
     1681 actgtcgaga cttaatgact ccaccccgaa cacggcttga gcctcattaa aatgattccg
     1741 caccagggct agaagcagaa actccgcccc cagacaggtc ccgcccatgg actccacttc
     1801 ctccagggcc agggagaaca ctcgcaagca ttcccgatcc aggcagtcca ttaccttctg
     1861 ctcggggaaa cccaactctt ccttttcaga caggccatca ctgaagcgag aatcaccgcc
     1921 cttttgatcg agcaaatacc actgccgcac cagctccact tccagactgt ctctggagcg
     1981 taaggatgta agcagagcaa tagccctgaa gaactgctcc tgcttgagca agcctggatc
     2041 tatgccgaag gacatgaact tctcagccag atctgccctg gaagtgagcg ccctcgccag
     2101 aggctcgcca tagagccggg caaaggggcc gccagcagaa agcaggtaag aagccaactg
     2161 ctcaggtccg gctatctgcc ggatcaaggc ttcctccggc ggttccagat aatcgatcac
     2221 agcctgcaaa tacaactgaa acgatagtaa gtccggagag ccctcagttt cattacatac
     2281 aggaatggtg agagcgctgc cgtccttaag caaaaggtcg atggtaccct cctccaactc
     2341 ctgcggcagt tcaaaactct tgagagagga ataggggacg aaacggctga cctcaccata
     2401 aatccagtgc aagccaaaat tgctgaccag aatgaagtcg cgcacatcgt tcatgtagac
     2461 gcccagcagt acttccttac tgtcaagaat ctcgcgactg atcacagccg cgttcttagg
     2521 catgacaaaa ctgtcaagaa gcgagagctg aaatagcttt tctcgtggag aagcgtatga
     2581 catttgccac cagagtcact gaaacagcaa attctagcag atagaagcat atgcccatca
     2641 gaaggctcaa ccgcgccgga tgcgatagcc gattttcggt actgtttcaa tgaaggaact
     2701 gccctcatcc tcgctttgat caattctctg ccgcagccgc ttgatacagg cccgcaccgc
     2761 ctctctggaa gagtcggaat cggtgtgcca gagcttctgc aagagcgctt cactactgaa
     2821 cacctggttc ttattgcgca tcaaaaattc gagcacggca aactcacggg gcataagctc
     2881 aatgtcttca ccacccctcg tgacacggtg gctctcagca tctaacacaa tatcgccggc
     2941 ctgcaaaacg ttgcaagcca ccccggccga acgccgcaca atcgcccgaa tgcgggcgct
     3001 caactctttc aaactgaaag gcttggtcag atagtcgtcg gcaccgcaat caagaccggt
     3061 aaccttatgt tcttcgctgt cccgcccggt caacatcaag atgggcactt tattctgagc
     3121 catgcgcaag cgcctgcaga tatcgatacc gctaagaccc ggcaattccc aatctagaat
     3181 gattacctca taactactga aggtaattag ctccagagcc tgattgcctt ccgccacgtg
     3241 ctccactaca tgcttttcgc ctttgagcca caactgtata gaatgggcca gcaccaagtc
     3301 atcttccaca agtaatactt tagccattgc aa
//
LOCUS       DAOQNZ010000271         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14174_length_3331_cov_3.821123, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000271 DAOQNZ010000000
VERSION     DAOQNZ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14174_length_3331_cov_3.821123"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKW73_06160"
     CDS             <1..329
                     /locus_tag="PKW73_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72899.1"
                     /translation="LAALEGKFTAWQEQNQQLVDNCDLLLGASGNSVFPEIPLSLENL
                     TMGGDWLKMAMEAGRNGKLRESRLYLKAASKALNQAEKVLAKKVRLNRRSALIKPPHY
                     LKSIAN"
     gene            complement(357..587)
                     /locus_tag="PKW73_06165"
     CDS             complement(357..587)
                     /locus_tag="PKW73_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72900.1"
                     /translation="MFTRKENNLDGQGLESGEDTGTGTLLWVLLWALALIVVQQWYAR
                     THNYGIEQPVGLFGLAEYFFRHPEKLICLLRY"
     gene            complement(703..1797)
                     /locus_tag="PKW73_06170"
     CDS             complement(703..1797)
                     /locus_tag="PKW73_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72901.1"
                     /translation="MLTPEYRLCEEGLKQSRMNDSDKALQLFTKAISCNSGYARAYLN
                     RGCTFRLAKQDMPARQDFKRVTQLINAPRSCADHFMLGTALQELGKHEAAISHLQAAT
                     ALKPLTLLDRMCRSRSYIALGDLENANLAISDYLQENPQSAPAYVLRADISTLTEARL
                     EQAVQDLSRAIALEPAYRTALTKRAALYNRMGKYSSGFEDGKTASLLEPDNPDVLLSL
                     GISHQGLGEPQAAMAAFKKVISLDETWNVPYLYLADLNSLQGHDGIALSLATRAIELE
                     PQDPNGYFCRARILHKANRLTEALQDVNQGIQRDNHRGLRGSNLRDKIEKALDEQSRL
                     KAILLLFLNGAVLFGAFSGRREQTTPALSN"
     gene            complement(2059..2208)
                     /locus_tag="PKW73_06175"
     CDS             complement(2059..2208)
                     /locus_tag="PKW73_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72902.1"
                     /translation="MHPLDFPLISYFVDWKNVTGTYILGALGVLACLITCVICWYQIF
                     NGAAD"
     gene            complement(2324..2836)
                     /locus_tag="PKW73_06180"
     CDS             complement(2324..2836)
                     /locus_tag="PKW73_06180"
                     /inference="COORDINATES: protein motif:HMM:NF020395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family c-type lysozyme inhibitor"
                     /protein_id="HNA72903.1"
                     /translation="MLDYGSIVKSDTFAHEVAVKTSANKPVRLIVLALTTSSILCLSG
                     VASAREMKETVYDLVSKAQYKPAWNTLFVGSKNLPGWIKKGEGVSSPCEEIKAAGGTY
                     IVGWMCEPHNCGNHQFAVAFSKDLKKAWGMRYVLEDVPPGKQAPAPKTTWFGNPSPEI
                     KKLLEQKLSQ"
BASE COUNT          747 a          797 c          891 g          896 t
ORIGIN      
        1 gattggctgc tctggagggc aagtttacag cctggcagga gcagaaccaa caacttgtgg
       61 ataactgtga tttgttactg ggagccagtg gtaattccgt ctttccggag atcccgcttt
      121 cactggaaaa tctgaccatg ggcggtgatt ggctaaaaat ggcaatggaa gctgggagga
      181 acggcaaact gcgcgaaagc aggctctatc tgaaggccgc gagcaaagcc ttaaatcagg
      241 cggagaaggt gctcgctaaa aaggttcgac tgaacaggcg ctcagcccta atcaagccgc
      301 cgcattacct aaagtcgatc gcgaattaga gcctatcctc aagatgtttc ctctctctag
      361 tatcgcaaga ggcatatgag tttttccgga tgacgaaaaa aatattcggc caggccaaag
      421 agacccacgg gctgttcgat gccgtagttg tgcgtgcgag cataccactg ctgaaccact
      481 atcaaggcta aggcccatag aagcacccac agtagtgtgc ctgttcccgt atcttctcca
      541 gattccaggc cctggccatc gaggttgttt tcttttctcg tgaacatgct acttccctgc
      601 gtccttgtgc gctgttgtct ttattagaca atcagggcgc aggattcagc gaggtagtgc
      661 cacagtttcc caactgttca gagtgattga ccgtttggta tctcaattgg atagagcggg
      721 agtggtttgt tctctcctgc ctgaaaaagc accgaagaga acggcgccat tcagaaacag
      781 tagcaaaata gctttaaggc ggctctgttc atcgagagcc ttttcaattt tgtccctcag
      841 gtttgacccg cgcaagccac ggtgattatc ccgttgtata ccctggttca cgtcctgaag
      901 cgcttcggtg agacgattcg ccttgtgcaa aatgcgggct cggcagaaat aaccattggg
      961 gtcctgcggt tccagttcga ttgcccgtgt tgccagactg agcgcaattc cgtcgtgacc
     1021 ttgcaggcta ttcagatctg ccagatagag gtagggaacg ttccaggtct catctaagga
     1081 gattaccttt ttgaaggccg ccatggcggc ttgcggctct cccagtcctt gatgggaaat
     1141 tcccaggcta agcaaaacat cagggttatc tggttccagt agggaggccg tttttccatc
     1201 ctcaaaacca ctggaatatt tacccattcg attgtagagg gcggctcttt tggtaagggc
     1261 cgttcgataa gccggttcca gggcgatagc cctgctcagg tcttggacgg cttgctcaag
     1321 tctggcttcc gttagtgtac ttatgtctgc cctcagtacg taggccggcg cgctctgcgg
     1381 attttcttgc aggtaatcgg aaattgccag gttggcattt tctagatcgc ccagggcaat
     1441 gtaggagcgg gaacggcaca tgcgatccag caaggtaaga ggtttaagag cagttgctgc
     1501 ttgcaggtga ctgatggcgg cctcatgctt gcccagttcc tgcaacgccg ttccgagcat
     1561 gaaatgatcg gcacaactcc ttggagcgtt gataagttgc gtgactcgtt tgaaatcctg
     1621 cctggccggc atgtcttgtt tggcaagcct gaaggtgcag cctctgttaa ggtaagctct
     1681 ggcgtagcct gaattgcagg aaatggcttt ggtgaagagt tgcagggcct tgtccgagtc
     1741 attcatgcgg gattgtttaa gcccttcttc gcaaaggcga tattcgggcg tgagcatgtc
     1801 attgcctgct ggtcttcctt ctccggtagc ggcaacattt ccagcactga ctgccgggat
     1861 gcaggttagt gccagaatta caattgaagt tgcggacaag gtttttgttt tcatgaaaca
     1921 gtccacgtcg gttcattagg ctaaggctga gttccttgcc ggatttataa atcttggcga
     1981 gttctatatc ggtcttaccg ccttttctgt ctgtccttct ttctgtctct gtaactatcc
     2041 taccggaagc tacatgcttc agtcggcggc gccattaaag atctggtacc agcagatgac
     2101 acaagttata aggcaggcaa gaacaccgag tgcccccagt atgtaggtac cggtgacgtt
     2161 cttccagtcg acaaagtatg aaatgagagg aaagtccagc ggatgcattt gcgggtagac
     2221 caattcacgg aatagacaaa acagccctga ccgtttagtt tgccacagtc tgccttgctt
     2281 gaaaatggta gagttacgct gaaactggag cccggtgtgg tttctactgg ctcagttttt
     2341 gctcgaggag cttcttgatt tcaggactgg gattgccgaa ccaggtggtt ttcggtgctg
     2401 gtgcttgttt cccgggtgga acatcctcca ggacgtagcg catcccccat gcttttttga
     2461 gatccttact gaaagctaca gcgaactggt ggttgccgca gttgtggggt tcgcacatcc
     2521 agccgacaat ataggtgccg cccgccgctt taatctcttc gcaggggcta ctcactccct
     2581 cacccttctt tatccagccg ggaagatttt tgcttccgac aaagagggta ttccatgccg
     2641 gcttgtactg tgcttttgaa accaggtcat agacggtttc tttcatttct ctggcggagg
     2701 cgacgccgct caaacacaga atcgagctcg tggtgagtgc aaggactatt aatctgaccg
     2761 gtttatttgc agatgttttc acggcaacct cgtgggcgaa tgtgtctgat ttgacgatgc
     2821 tgccataatc taacacagtt accggcgtag acagcgggaa atcctgcgga cggttgccgc
     2881 ctttgagagt agacttatcg acctataaac atctgggtcc tggtgtctct tttaaacaag
     2941 caggcaaatg taacagcgat tccgcagagt cgctgccagc acaggacgtc aatccggtat
     3001 ctggtatctg cagagaatag ctatatacga gtgctccccg ctacaaagtg ctgaaattgc
     3061 tgtcagggac ggcttgggta gtcattttgc ggaatcgctg caaatgtaac aatagtgatt
     3121 ttgcgaaata ttacatcagg gttttcagtc tttttgagat ttgaaaccgt tgcagcataa
     3181 gccttttggc tgtcaaacag atttaatttg cggctttaca tgagtttagc gcttttggga
     3241 agatttttcg caggacatac ctgcggtctg caggtcggtc attttcggca ccggcttcaa
     3301 atttggtcga aacgcttttg gatagggcgc t
//
LOCUS       DAOQNZ010000272         3328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14200_length_3328_cov_3.150626, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000272 DAOQNZ010000000
VERSION     DAOQNZ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14200_length_3328_cov_3.150626"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..259)
                     /locus_tag="PKW73_06185"
     CDS             complement(23..259)
                     /locus_tag="PKW73_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72904.1"
                     /translation="MEPKNFKEPSEKIAEEAPRLREVKDESPQLPAQKNTSRSLADTL
                     RLTKSRILPPVDAPPRTLLPRRLAAHAIDACEKC"
     gene            460..1809
                     /locus_tag="PKW73_06190"
     CDS             460..1809
                     /locus_tag="PKW73_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNA72905.1"
                     /translation="MYQGLIPGHPPGLSVLFFTELWERFSYYGMRAILVLYMTASLAN
                     GGLGFSVEKSSEIYGNYTASVYLVSIFGGLLADHFLGPRRAILIGGIIIALGHFTMAF
                     PSTYAFFTGLVLIVMGTGFLKPNISTMVGKLYPPGDPRRDSGFSIFYMGINIGATLAP
                     FACGYLAQSDHFKGVLKSFGLSPELSWHFGFAAAGVGMCLGLFHLLIQYKILDAVGER
                     SGKAEESSQEPAAKIKLTVDEWKKLGAIACLFVFNVLFWSIFEQGGSSLNLFAERLTN
                     NTFLGFKFEASYLQSLQAADVIILAPIFSYIWIKLGTKAPSSPAKFALGLMFLGLGIA
                     FMVPAAMLTAQGKVSPLFLVAVYFTETLGELCLSPVGLSTVTKLAPRAFQSLTMGAWF
                     ISTAIGNKLAGHYSGHFKEDPQSMIFLFGAMALAALAASAVLALLTPSIKKLMGDVK"
     gene            complement(1813..>3328)
                     /locus_tag="PKW73_06195"
     CDS             complement(1813..>3328)
                     /locus_tag="PKW73_06195"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HNA72906.1"
                     /translation="KSSGFTDVLLLGMGGSSLAPEVFSVTYGPAAGYPRLHILDSTDP
                     QQIAHVQAGLETKKTLFVVASKSGSTLEPSIFMKHFLERAKGELGDNFGAHFVAITDP
                     GSKLEAEARALNFRNIFHGLPGVGGRYSALSPFGVVPMCLLGMDVRQFLQIAADMAEA
                     CKLAGADNPGLHLGAVMGEANLVGRDKLTIFTSPSIYDFGAWLEQLVAESTGKVGKAI
                     IPVDQELPGAMELYGKDRLFVFVKLAGDKTDHGLQNEEVEKFLSTAEAAGHPVITITL
                     KDKLHLAGEFFRFEMATAVAGAVMGINPFDQPDVEASKIETRELTDAYEKTGKLPAEE
                     PFFTAGQIELYSSVENAEAITGGAKQQTLSGMLASHLQRLNSGDYFALLAYLFMDHKE
                     IESTLFKLRMAVRDKKQVATCLGFGPRFLHSTGQAYKGGPNSGVFLQVTADDAEDLPV
                     PGARYTFGAVKAAQARGDFRVLLDRKRRALRVHIKGDLKTGLESLKTAMLEALS"
BASE COUNT          748 a          927 c          840 g          813 t
ORIGIN      
        1 tgagcctttt aaaattgctc cattaacact tttcacaggc atcaatggca tgggcagcga
       61 gacgcctcgg caggagagtg cggggcggcg catcaacagg cggtaagatg cgacttttcg
      121 tgagccgaag cgtatcagcc agggaacgac tggtgttctt ctgtgccggc aattgcgggc
      181 tctcgtcctt gacttcacgc aggcgcggcg cttcctccgc gatcttttca gagggctcct
      241 taaagttctt cggctccata tccatatagc tgctttaatc ctaactggct ggccgacaat
      301 ctttcatttt gcacccggat tgacttaatt gcctaccgga atctggtaaa acttaggggc
      361 attctatgat cacaaccaga ttgtagtact taagtcattc ggctaagtga tagaaaagca
      421 gtcttttggt taagcaacca attttcaaga aggagtcaaa tgtaccaggg gcttattcca
      481 ggtcatccac cgggtctttc cgtactgttc ttcaccgaac tctgggaacg tttcagctac
      541 tacggtatgc gtgccatcct ggtgctttac atgactgcca gcctggctaa tggtggtctt
      601 ggcttctccg tagaaaagtc ttccgagatc tacggcaact atacggccag cgtctatctg
      661 gtttccattt tcggtggtct gctggccgat cactttcttg gtccccgccg ggcaattcta
      721 atcggcggca tcatcatcgc cctcggtcat ttcaccatgg cctttccttc cacctatgcc
      781 tttttcacag ggctggtctt gattgtgatg ggaacaggct ttctaaaacc aaatatctca
      841 accatggtag gaaaactcta cccaccggga gacccgcgcc gagattcagg cttttccatc
      901 ttctatatgg gcatcaatat cggcgccact cttgctccgt ttgcttgcgg ttacctggcc
      961 caatcagacc acttcaaagg cgtgctcaaa tcctttggac tctccccgga gctaagctgg
     1021 catttcggct tcgccgcagc cggtgtgggt atgtgcctgg ggctatttca tctactaatt
     1081 caatataaga ttctcgatgc cgtgggcgaa cgcagcggca aggctgagga aagctcgcaa
     1141 gaaccggccg caaagatcaa attgaccgta gatgaatgga agaaactggg cgccattgcc
     1201 tgcctcttcg tcttcaacgt actcttctgg tcaattttcg agcagggcgg ctcatctctc
     1261 aatttgtttg cggagcgcct caccaataac accttcctcg gtttcaaatt cgaagccagc
     1321 tacctgcaat ctctgcaagc tgccgatgta attattctgg ctcccatatt cagctatatc
     1381 tggattaaat tgggcacgaa ggccccttcc agcccggcca aatttgccct gggtctgatg
     1441 tttctcggac tgggtatcgc ctttatggtg ccggcagcca tgctaacggc tcagggtaag
     1501 gtcagccccc tcttcctggt ggctgtctat ttcactgaaa ctcttggcga actctgcctc
     1561 tcgccggtgg ggctttccac cgtgaccaag cttgctccca gggccttcca gagtttaacc
     1621 atgggagcct ggttcatttc cacggccatc ggcaacaaac tggccggaca ttactccggc
     1681 cacttcaagg aagatcctca atctatgatc tttctcttcg gcgccatggc cctggcagcc
     1741 ctggccgcca gtgccgtctt agcgttactt acgccatcca tcaagaaact aatgggcgat
     1801 gtcaaataag ttctaggaca gagcttcgag catcgcggtc ttgagggact caagcccggt
     1861 tttcagatcg cccttgatgt gaacgcgcaa ggcacgacgc ttgcgatcga gaagtactct
     1921 gaaatctccc cttgcctgag cagctttgac cgcgccgaaa gtatagcggg ctcccggtac
     1981 aggcaagtct tcggcatcat cggcggtgac ttgcaggaag acgccgctgt tgggtccacc
     2041 tttataggcc tggcctgtgg aatgcagaaa gcgaggacca aagccgaggc aggtcgccac
     2101 ctgtttctta tcacgaacgg ccatacgcag cttaaagagc gtcgattcaa tttctttgtg
     2161 atccataaac agataggcga gcaaggcgaa atagtcaccg ctattcaaac gctgcaaatg
     2221 agacgccagc atgccgctca gagtttgctg cttggcaccg ccggtaatgg cctcggcgtt
     2281 ctcaacggag ctatagagct caatttgacc ggccgtgaag aagggctcct ccgcaggcaa
     2341 tttgccggtc ttctcatagg catcggtaag ctctctggtt tctattttgc tggcttccac
     2401 atcgggttga tcgaaggggt tgatgcccat gacggctccg gcaacggcag tggccatctc
     2461 gaagcggaag aactctccgg ccaggtgcaa tttatctttc aatgtaatgg ttatgacagg
     2521 atgaccggca gcttcagcag ttgaaagaaa cttctcgact tcctcgtttt gcaaaccatg
     2581 gtctgtttta tcaccggcca gtttcacaaa cacaaacaag cggtccttgc cgtaaagttc
     2641 catagcaccg ggcaattcct ggtcaaccgg aataatagcc ttgccgactt tgccggtcga
     2701 ctcggccacc agttgttcca gccaggcacc gaaatcgtaa atagacggac tggtgaaaat
     2761 cgtcagctta tctctgccga ccagattggc ttcgcccatg acggcaccca gatgaagacc
     2821 ggggttatcc gcgccggcaa gcttgcaagc ttcagccatg tccgccgcta tctgcaggaa
     2881 ctgccgaaca tccatgccga gcaggcacat aggcaccacg ccgaagggtg aaagggctga
     2941 atagcgtccg cccactcccg gcaagccatg gaagatattt ctgaaattaa gagcgcgggc
     3001 ttccgcttcc agcttggagc caggatcggt aatagccaca aaatgcgcgc cgaaattgtc
     3061 gcctagctcg cccttcgccc tctccagaaa atgcttcatg aaaatagaag gctccagcgt
     3121 gctgcccgac ttgctggcca ccacaaataa agtttttttc gtctcaaggc cagcctgcac
     3181 atgggcaatt tgctgagggt cggtggaatc aaggatatgc aggcgtggat agccggcggc
     3241 tggtccgtaa gtaacggaaa agacttccgg cgccaggctg gaaccaccca tccctagaag
     3301 caaaacatcg gtaaatccgg aggattta
//
LOCUS       DAOQNZ010000273         3328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14201_length_3328_cov_2.804461, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000273 DAOQNZ010000000
VERSION     DAOQNZ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14201_length_3328_cov_2.804461"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKW73_06200"
     CDS             complement(<1..463)
                     /locus_tag="PKW73_06200"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNA72907.1"
                     /translation="MSLLSYLRLAAAYIKLNWRMQMEYKSAFASQFLAMVLNNSTWLA
                     FWLIFFDRFPVVRGFGQADVVTLWAIASAGFGLSAACCFNLHNLAALIARGQLDTWML
                     YPRALVPHLALGKLSPTAMGDLAFGYAAYLLLVKPDLEPFLMYVFLTVAVAV"
     gene            complement(665..1474)
                     /locus_tag="PKW73_06205"
     CDS             complement(665..1474)
                     /locus_tag="PKW73_06205"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNA72908.1"
                     /translation="MLKTAQKLIAVAKISARANLAYPGEVLGRGTFLLVVLYIFLKLW
                     QATYSESGQSKLAGLSLAEMLWYLTITESMWMSAPKLAQQVDEDVRTGSLAVKLIRPL
                     SYPLYCLSANMGERLVRFSFNLALGACIALALVGPIHLRAEALLMLALSVCLAFLLDG
                     LGHLIVGLSAFWLEDTSGLYLIYTRLTMILGGMLIPLDLFPGWLKNLVVYLPFPYLVW
                     GPVHLLSTPQTELAASFLELAIRQLACLVALSALVALLWQRALRRVSAHGG"
     gene            complement(1485..2291)
                     /locus_tag="PKW73_06210"
     CDS             complement(1485..2291)
                     /locus_tag="PKW73_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA72909.1"
                     /translation="MFAVHTEDLRKEFSLKKGLFERRTAVAVDNISLTVKEGQAVAFI
                     GPNGAGKSTTIKMLTGILKPSSGKAQVLGLDPWRQRSRLVRQIGAVFGQRSQLWLHLP
                     PKDSFELLAEIYSLDKKSKQYKSRLEMLIERFELAAFLNTPVRKLSLGERMRAEIAAS
                     LLHRPRLLLLDEPTIGIDVVARQRLRELLAQWNREEGLTVFLPSHDAGDIEAVADRVI
                     LINHGRVIIDDTIDGVRHYGAPKSADTGEPPDMQTLPALEKVLSDLFTAK"
     gene            2441..2581
                     /locus_tag="PKW73_06215"
     CDS             2441..2581
                     /locus_tag="PKW73_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72910.1"
                     /translation="MFSILSAPVSYDLLAVAKAGLGWYNHDLFNDGKDFSHGVHASEI
                     KG"
     gene            2790..>3328
                     /locus_tag="PKW73_06220"
     CDS             2790..>3328
                     /locus_tag="PKW73_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72911.1"
                     /translation="MCCSVSLAASGSLPVVQASPEPAPSRAEPVPSRSELARLSQAEL
                     IRLNNPFDKQYFQLIDALVLKYPHSPDLHLQRGLALADCSRWSESLKDLNEALVLDKS
                     LSGSAALHFQSGNCYAGLKQYEKAAREYELSLAKYGTAGADKAEMQNCLLRLAKTYFQ
                     LGKREACAKAYYKAAGLTDP"
BASE COUNT          758 a          845 c          946 g          779 t
ORIGIN      
        1 gtactgccac tgctactgtc agaaagacat acatgagaaa gggctccagg tccggtttaa
       61 ccagcaagag ataggctgca tagccgaagg ccaggtcacc catggccgtg gggctgagct
      121 tgcccagggc cagatgcggc accagggctc tggggtagag catccaggtg tcgagctgac
      181 ctcgggcaat tagcgcggct agattgtgca ggttgaagca acaggccgcc gatagtccga
      241 agccagccga ggcgatggcc cagagggtca ctacatcggc ttgaccgaag cctctcacaa
      301 ccggaaagcg gtcgaagaag ataagccaga aggcaagcca ggtggagttg ttcaagacca
      361 tggccagaaa ctggctggcg aaggcacttt tgtattccat ttgcatgcgc cagttgagtt
      421 tgatatatgc agccgccagg cgcaggtaag acagcaatga catgattttc cccctttgtg
      481 tggagcggat agtcgtttgg acctgagcat cctttgtgtt tggtgtttga tttgagtgtt
      541 cgtttagacg agactcagtg ttcgtttaga cgagactcag tgttcgttta gacgagactc
      601 agcgttggtt tcgacgagac tcagcgttcg tttagacgag actcagcgtt ggtttcgacg
      661 agactcagcc cccgtgggcg gaaacgcgcc gcagcgctct ttgccagagc agggccacca
      721 gggcggagag tgctaccagg caggcaagtt gccgaatggc aagctccagg aaggaggccg
      781 ccagttccgt ctgcggtgtt gaaagcagat gcaccggccc ccagaccaga taaggaaagg
      841 gcaggtagac aaccagattc ttcaaccagc caggaaagag atccaggggg atgagcatcc
      901 cacccagtat catggtcagt ctcgtgtaga tcaaatagag accggaggta tcttccaacc
      961 agaaggctga tagacctaca atcaagtgac caagcccatc taagaggaag gccaggcaca
     1021 ccgataaggc aagcatcaaa agcgcctccg ctctcaaatg aattggaccg accagggcca
     1081 gcgcaataca ggctcccaga gccagattga aggagaatcg caccaggcgc tcacccatgt
     1141 tggcgctaag acagtagagc ggatagctga gtgggcgaat cagcttcaca gccagactac
     1201 cggtgcgcac gtcttcgtcc acctgctgag ccagctttgg cgccgacatc cacatggatt
     1261 ccgttatggt caggtaccag agcatctccg ccaggctgag cccggccagt ttagactgcc
     1321 cgctttcact gtaggtagcc tgccagagct tgaggaagat gtacaggaca accaggagga
     1381 aggtgccccg accgagcacc tcgcctggat aggcgaggtt ggctcgggcc gagatcttgg
     1441 ccacggcaat tagtttttgt gctgttttta acatgactac cttcctattt ggcagtgaac
     1501 agatctgaga gcaccttctc cagagctgga agagtctgca tatcgggcgg ctcaccggta
     1561 tcggcacttt tgggtgcacc atagtggcgc acaccgtcta tggtgtcatc aatgatgacc
     1621 cgtccgtggt ttatgaggat gacccggtct gctaccgctt cgatgtcgcc ggcgtcgtgg
     1681 ctggggagaa agacggtcag tccttcttcc cggttccact gagcgagaag ctctctcagc
     1741 ctttgcctgg caaccacgtc tatgccgatg gtgggttcgt ctaagagcag taaccgcggg
     1801 cggtgcagca ggctggcggc gatttcggca cgcatgcgct ctcccagaga gagtttgcgc
     1861 acaggcgtgt tgagaaaggc agccagttcg aagcgctcga ttagcatctc cagtctggat
     1921 ttgtactgct tcgatttctt gtcgaggctg tagatttcgg ctagaagttc gaagctgtcc
     1981 ttgggaggca ggtgcaacca gagctgcgag cgctgtccga agacggcccc tatctgcctt
     2041 accagtcggc tgcgctggcg ccagggatca agtcccagta cctgcgcttt tccggagctt
     2101 ggtttgagta tgccggtaag catcttgata gtggtggatt tgccggcgcc gttaggaccg
     2161 ataaaggcca cagcctgacc ctctttcacc gtcaatgaga tgttgtctac ggctaccgct
     2221 gtgcggcgct cgaagagtcc cttcttgagg ctaaattctt ttctcaagtc ttcggtgtga
     2281 acggcgaaca tggtcttcct tcctttctgt ttttggtatc tgcgtttctt cgttttgagt
     2341 tttgcgactg tcttacgggc atagcagtag ccgtctcatg ggcctcagac caatgagcat
     2401 aaaaaaggct cccgtaagtg ggagccgcaa tcagttcagg atgttttcta tcctaagcgc
     2461 tcccgtttcc tatgacctgc tcgccgtagc aaaagccggt ctgggctggt acaaccatga
     2521 tttgtttaac gatggcaagg acttcagtca tggtgttcac gcttctgaga taaaaggttg
     2581 atttgttgat tggacgcttt tctattatgt tgaaactgta gctcaggtca atgctttgta
     2641 aagttactgg tttccaaaat ctgaaagtct tggtacaggc gagttttccc tgaccagttg
     2701 agcaaaggtg tcataattag tccatgatca ggctcaaggc gagacttctt aaaaggatag
     2761 gcttaccaga aggttggctc aagacgctct tgtgttgttc cgtcagtctg gcggcatcag
     2821 gctctttacc ggtagttcaa gcttcgcctg agcctgcacc aagtcgggct gagcctgtac
     2881 caagccgcag cgaacttgcc agattgtctc aggctgagct gattcggtta aacaatccat
     2941 ttgacaaaca atattttcaa ttgattgacg cgcttgtgct taagtatcct cactcgcctg
     3001 acctacattt gcaacggggt cttgctctgg cagactgctc ccgctggagc gagtctttga
     3061 aagacttgaa tgaggcgctg gtattggata agtcactgtc tggttcggct gccttgcact
     3121 ttcaatcggg taattgttat gcaggtctca aacaatacga aaaagctgcc agggagtacg
     3181 aactgagtct ggcaaaatac ggcaccgccg gcgcagacaa agcagagatg cagaactgct
     3241 tgttgcggct ggcaaagaca tactttcagc tcggtaaacg ggaagcctgc gctaaagcct
     3301 attacaaggc agccggcttg actgatcc
//
LOCUS       DAOQNZ010000274         3327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14210_length_3327_cov_3.527812, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000274 DAOQNZ010000000
VERSION     DAOQNZ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14210_length_3327_cov_3.527812"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PKW73_06225"
     CDS             <1..1056
                     /locus_tag="PKW73_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72912.1"
                     /translation="TLPGFRKSFQAQFLRAQCSHAAGDHQEAVRDLEAALKIKKDDGA
                     RELLAISYAHLGKAGAARELLAVQLKSDAPSVQTLLAAARVEQVLGQELSAIDFYSRV
                     LLRKPRQVEALVGRADCYLARKQYASAQADLSLAVEEGRGDKKTVENLLFCQSVLKKQ
                     AHIAIDLPKSRSFNFKSVDSVALFDKALKAYRQNDADLCIAACYELTGREPAHSGAWR
                     LLAHTLQECSRNQEAIDAFGNLAALENPTAADKLKLARALSATQQHEQAIAILTEQHK
                     LDRANGELTAELAKIYAASDRKDEAMKLCRQAIEGGKGSDQALVQIKNLYQSLLAEKE
                     RANGANDGNNVVQDPQG"
     gene            1053..1718
                     /locus_tag="PKW73_06230"
     CDS             1053..1718
                     /locus_tag="PKW73_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72913.1"
                     /translation="MNMKPAIGHSYLKEQPGEPLGAGISGRLAESKLGLSRLLGRLPR
                     GFSTEVALSLAILTYLFYQITWLCEDSELKKRILEPVRQTWLFWGLDENWRLFSPVIK
                     DINFHTLAVITFEDGTKKLWELPRMKRLNYIERFKDEKYRKWCVDSLPWPDYKEFYPD
                     FARYVGRKFYNPANKPASLTLLLYWIEIPDPKTKFTPVAALPEHSKMNTVFYYRYKPE
                     DLP"
     gene            1715..2656
                     /locus_tag="PKW73_06235"
     CDS             1715..2656
                     /locus_tag="PKW73_06235"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HNA72914.1"
                     /translation="MNVSNLWRIWRGFWFEPSGTETISVYRILFGLLVMQTSIVHLSG
                     RFSEWYSDKAMITLPTVINHFWFREPRFDLMLLFPQQEPTFTIFYYVLVFFSLALTFG
                     FHGRFSAMMVWLLLLSMHHHNPYNINGGDAFLRVVAPFLALSPCSDHYSIDAWLRSRR
                     GETVPELKMPWAQRLIQVSIALAYWQTFWCKISGNQWLDGTAVYYATRLDDMLRFPAP
                     WISDNMLVLQVLNWFTLIIEFLGWNAIFIKEFRYYVVVGLVFLHLGIDYMINLPVFEW
                     AFIFTLFTFVEPAHVRLAISKLKELMPPVRRPVPTGS"
     gene            2956..>3327
                     /locus_tag="PKW73_06240"
     CDS             2956..>3327
                     /locus_tag="PKW73_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72915.1"
                     /translation="MSEKPAEVPAKKAPEVAKVGDHNSGDGHKGAGLHQEAATVLSSQ
                     GGNDAHKAAQAKEGSGVGHDVTIVENGKPVAKGGTVDGAADLKPAHKAAEHHVVHKEA
                     HKPASAEAIVALEPDLVLAAEI"
BASE COUNT          721 a          912 c          881 g          813 t
ORIGIN      
        1 actctgcctg gcttccgcaa gtcttttcag gcacagttcc tgcgggcgca gtgctcgcat
       61 gccgctggcg accatcaaga agccgtgcgt gatctggaag cggctttgaa aatcaagaaa
      121 gacgacggcg cccgggagct actggccata agctacgctc acctgggcaa agccggagcc
      181 gctcgggaac tgcttgctgt tcaactcaaa tccgatgcgc cttccgtcca gaccttactg
      241 gctgccgcca gagtggagca ggtgctcggc caggaactct ccgccattga tttctacagc
      301 cgggtgctgc tccgcaaacc ccgccaggtg gaagccctgg tgggccgtgc cgattgctat
      361 cttgcccgca agcaatacgc cagcgcccag gctgacctca gtctggccgt agaggaaggt
      421 cgcggtgata aaaagactgt ggagaacctg cttttctgcc agtctgttct gaagaagcaa
      481 gcgcatattg ctatcgattt accaaagagc cgttctttca attttaaatc ggtagattcc
      541 gtggcgctct ttgataaagc cttgaaagcc tacagacaaa atgatgccga cctgtgtatt
      601 gccgcctgct acgagttgac cggtcgcgag ccggcccata gcggcgcctg gcgcctgctt
      661 gcccacaccc tgcaagaatg tagccgcaat caggaagcaa tcgatgcctt cggcaattta
      721 gccgctcttg agaatcccac cgctgccgac aagctcaagc tggcacgggc tctcagtgcc
      781 acgcagcaac acgagcaagc catcgccatt ttgactgaac aacataaact tgacagagcc
      841 aacggcgaac tgacggcaga attggctaaa atatatgctg cttcagacag aaaggatgag
      901 gccatgaaac tttgccgcca ggccattgaa ggtgggaaag gctccgatca ggctctggtt
      961 cagatcaaga acctttacca gtcgctgctt gccgaaaagg agagagcgaa cggtgctaat
     1021 gacggtaata acgtagtgca ggacccacaa ggatgaacat gaagccggcc attggccata
     1081 gctaccttaa ggaacagcct ggtgagcctc ttggtgccgg gatcagcggc cgattggcgg
     1141 agtccaagct gggtctcagt cgtctacttg gtcgactgcc ccgaggcttt tccaccgaag
     1201 tggcactcag cctcgctatt ctcacttatc ttttctatca gatcacctgg ctctgtgaag
     1261 acagtgaact gaagaagcgc attcttgagc cggtgcggca gacctggttg ttctgggggc
     1321 tcgatgaaaa ctggcgtctt ttttcgccgg tcatcaaaga tatcaatttc cacactctcg
     1381 ccgttatcac ctttgaagac ggcactaaga agctctggga actgccccgc atgaagcgcc
     1441 tcaattatat tgagcgcttc aaagacgaga agtatcgcaa gtggtgtgtt gattcccttc
     1501 cctggcctga ctacaaagag ttctaccctg actttgccag atacgtggga cgcaaattct
     1561 ataacccagc caataagcct gcttctctca ccctgcttct ctactggatt gaaattcccg
     1621 accctaaaac caagtttacg ccggtggccg ccctgcccga gcatagcaaa atgaatacag
     1681 ttttttacta ccgctacaag ccggaggatc tgccatgaat gtgagtaatc tctggcgcat
     1741 ctggcgcggt ttctggtttg agcccagcgg tactgagacc atcagtgtct accgcattct
     1801 ctttggtcta ctggtgatgc agaccagcat tgttcattta tccgggcgct tcagcgaatg
     1861 gtattcggat aaggcgatga ttaccttgcc cacggtcatt aatcacttct ggttcaggga
     1921 accacgtttc gatttgatgc tgctcttccc gcagcaggag ccgactttca caatcttcta
     1981 ttatgtgctg gtcttctttt ctctggctct tacttttggc tttcacggtc gcttcagtgc
     2041 catgatggtc tggctcctgt tgctatccat gcatcaccat aatccttaca atattaacgg
     2101 tggtgatgcc ttcttacggg tagtggcgcc attcctggct ctttctccct gcagcgatca
     2161 ttattccatc gatgcctggt tgcgcagccg tcgcggcgaa actgtgcctg agttgaagat
     2221 gccctgggct cagcgcctca ttcaagtatc aattgctcta gcctactggc agactttctg
     2281 gtgcaagatc tccggcaatc aatggcttga tggaacggcg gtttactacg ctaccaggct
     2341 tgatgatatg ctgcgcttcc cggcaccctg gatttccgac aatatgttgg ttctacaggt
     2401 cttgaactgg tttaccttga tcatcgaatt ccttggctgg aacgccattt tcatcaagga
     2461 gtttcgctac tatgtggtgg tgggtctggt cttcttgcac ctgggcatcg actatatgat
     2521 caatctgccg gttttcgaat gggcatttat ttttacgctc ttcacctttg tggagccggc
     2581 tcatgtgcgc ctggctattt ctaaattgaa agagctgatg cccccggtga gacgcccggt
     2641 gccgacaggt agctgacgct gggccggtaa gctgtggctg ctcccttcgt caaatttaat
     2701 ctaccgtagc agtggggcct catgagcggg tggcgggctg ttgggcgcaa ccctgactgc
     2761 ctggcatttt cccgtggtag cctggattta gcggtcgccg cttctttcgt aatcgccgca
     2821 ttgaccgggg gcttgtctgg cattatcttt ggaacacccc gtggttatgt tcaggtccct
     2881 taaggtatcc tgcggcatgc cgcagaatta ccagcccctg tacctgaatg gtttgctaaa
     2941 ccacgaggaa tggtcatgtc tgaaaagccg gcagaggttc ctgccaagaa agctcctgaa
     3001 gtcgccaagg taggcgatca taatagtggt gatggtcata aaggcgccgg tttgcaccag
     3061 gaagcggcga cggtcctttc cagtcagggc ggcaacgatg cccataaagc cgctcaggcg
     3121 aaagaaggtt ccggcgtcgg ccatgatgtg accatagtcg aaaacggcaa gcccgttgct
     3181 aagggcggca ccgttgacgg ggctgccgac ctcaagccgg cacataaggc agccgaacat
     3241 catgtggtgc acaaggaggc acataagcct gccagcgccg aagccattgt tgccctcgaa
     3301 cctgatctgg tgctcgccgc tgaaatc
//
LOCUS       DAOQNZ010000275         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14320_length_3312_cov_3.673319, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000275 DAOQNZ010000000
VERSION     DAOQNZ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14320_length_3312_cov_3.673319"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..384
                     /locus_tag="PKW73_06245"
     CDS             184..384
                     /locus_tag="PKW73_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNA72916.1"
                     /translation="MEEFYTVKPGDCVWNIAREYLKRNGTEAPSDKEIMAETNRLVLL
                     NKLDEGGRNRDLIYPGENLKLN"
     gene            446..1693
                     /locus_tag="PKW73_06250"
     CDS             446..1693
                     /locus_tag="PKW73_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72917.1"
                     /translation="MQTISLKEKLDELKRLRRLGQWQAAEAVCEELLHEQVPPLDSPS
                     ISEESLNSALAVLRERACLYEQSSTNNRLWLESLQLIQALEKRACAPVSQQARTLWKA
                     HLCAKRMDYVAYHLQGEPHLHEALALFKDPSLLTDPTYLNLLGWHTAFRAGDLNRGVA
                     LMESALERYSQIKEERSEDMCFTYQLLSLAYDQNGCGDKLRLFLERGRALAQAMKEPA
                     ELADLLTFAAARQAVEPEFALDGLKWIEKNYGERHPKLLVLRTAIAGRLEAEAMDEAE
                     TYWLESLSKADEPPMGHAIYALTGYGGYLERQSRLSEAEDTYNRAMDIIESYFKPEPL
                     FFIGPQPALALAELLGNQGRLEESAELLRNFIARQEKDDYISALDVEYALETLINVLH
                     RLGRSHETEALMRKLNKMQGTES"
     gene            1786..2106
                     /locus_tag="PKW73_06255"
     CDS             1786..2106
                     /locus_tag="PKW73_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNA72918.1"
                     /translation="MAITDESKDKLKARLSKAIGHLNAVYKMVDEKKYCIDILNQLKA
                     VQSALDKTAEIMLKDHLNTCVVEAVQNNDSQRVMEELWQLLRHSEPETPEEKSTETSG
                     CCGQ"
     gene            2241..2876
                     /locus_tag="PKW73_06260"
     CDS             2241..2876
                     /locus_tag="PKW73_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72919.1"
                     /translation="MFKQSLALIQPKPLQTALPVCLALLSGLTGLFAEPAQAQTVVNI
                     KRDQLEQVKPFQARRQIYITDETPKVSDHRHPPEASPNYAIFIPPLASNAGPGQTILL
                     TPQPNGLPYAGFESNIGAGQKFARALPQVNIGGLSPNPAVRPVGAPGRTGSSRALTNT
                     QARPVGKPVTSSNQASQYQPYTPGNAAGSSINSYSSSAVSKVSLTGRLLRK"
BASE COUNT          911 a          936 c          792 g          673 t
ORIGIN      
        1 ctaaactgcc tagtctgttc cgaatcaatt tccttcaagg tcagtattcg gaaggcgaaa
       61 ttaaagcact gagactggct tatccaggcg ttcaactcaa tatcatagac ctgaatgata
      121 aagccgatcc cacagttttt gcaccacttc actgatccag aacaaactga cggagaatca
      181 agaatggaag aattctacac ggtcaaacca ggcgattgcg tctggaacat agccagagaa
      241 tacctcaagc gaaacggcac agaagccccc agcgacaaag aaataatggc agaaaccaac
      301 cgccttgtct tgctcaataa gctggacgaa ggcggtcgca accgcgatct catttatccg
      361 ggggaaaatc tcaagctgaa ttaaaagcgc aagagatgta taaaactggg catgatccgt
      421 gatattcact tcgagaagtg cggcaatgca aaccatcagc ctcaaggaaa agttggatga
      481 attgaagcga ctgcggcgcc tgggtcagtg gcaagcagct gaagccgtct gcgaagagct
      541 tctgcacgag caagtgccgc ccctggatag tccatcaatc tcggaagaat ccctcaattc
      601 ggctctggca gtgctgagag aaagggcctg cctctacgaa cagagcagca ccaacaatcg
      661 cctctggctc gagtcgctgc aacttataca ggctctggag aaacgtgctt gcgccccggt
      721 cagccagcaa gcccgcaccc tctggaaggc ccatctttgc gccaaacgca tggactatgt
      781 cgcttatcat ttgcagggcg agccgcatct gcacgaggct ctggccctat ttaaagaccc
      841 cagcctgctc actgacccca catatttgaa cttacttggc tggcacacag cttttcgcgc
      901 tggtgattta aaccggggcg tagcgcttat ggaaagtgcc ctggagcgct attcgcaaat
      961 caaggaagag cgctccgagg acatgtgctt cacctaccaa ttgctaagcc tggcctacga
     1021 ccaaaacggc tgcggcgata agttgagact cttcctggaa agaggacgag ccctggctca
     1081 agccatgaaa gagccggccg aactggctga ccttttgact tttgcggcgg ccagacaggc
     1141 agtagagcct gagtttgctc tcgatggctt gaagtggatt gaaaaaaact acggagaacg
     1201 ccatcccaag ctgctggtgc tcagaacagc cattgccggc cgtctggaag cggaagccat
     1261 ggacgaggcc gaaacctact ggctggagtc gctttccaaa gccgatgaac cgcccatggg
     1321 tcatgctatt tacgctctca ccggctacgg aggctacctg gagcgccaga gccggctttc
     1381 ggaagccgag gatacctaca accgagccat ggacataatc gaatcatatt tcaagccgga
     1441 gcccctcttt ttcatcggcc cgcaaccggc ccttgctctg gcggagttgc ttggtaatca
     1501 gggccgtctg gaagagtcag ccgaactatt gcgaaatttc atagcccggc aggaaaaaga
     1561 tgactatatt tcagctctgg acgtggaata cgccctggaa acactgatca atgtgctgca
     1621 ccggctgggc agaagccatg aaaccgaggc tttgatgcgc aagctgaata agatgcaagg
     1681 cacagaatcg taaagctcgg tttctcaagc tcagattagc ttttcagcct ctcaagagaa
     1741 tttattaact tcaaaagcaa gccataaagg gtaaactcct ttcttatggc aatcaccgac
     1801 gaatccaaag acaaactcaa agccaggctc tccaaagcca ttggtcatct caacgctgtc
     1861 tataagatgg tggatgagaa aaagtattgc atcgacattc tcaatcaact gaaagccgtg
     1921 cagtcggctc tcgataagac ggccgagata atgctcaaag atcacctgaa cacctgtgtg
     1981 gtggaagcgg tgcagaacaa tgactcccag agggtgatgg aagaactatg gcagttgctc
     2041 cggcacagcg agccggaaac gccggaagaa aaatccacgg aaacctccgg ctgctgcggc
     2101 caatagcccg ctcccgccag tagaaaaaca agccgggtga gagcttcagg cattacccag
     2161 gtttcatgtc agggtaaatc ccagagcagc cgcctgacaa ctttcataca atgcggacac
     2221 tcaatcctgg gggttcggca atgttcaagc aatcactggc actaattcaa ccaaaaccgc
     2281 ttcagacagc cctaccggtc tgcctcgctc tcctttccgg tttgaccggc ctctttgccg
     2341 agcctgctca ggcccagact gtcgtaaaca tcaaaagaga ccagttggaa caagttaagc
     2401 cattccaagc ccgcaggcaa atatacatta ccgacgaaac cccgaaggtc tccgatcatc
     2461 gtcacccgcc cgaggcctcg cccaattacg caattttcat tccgcctctt gcgtcaaacg
     2521 ccggacccgg gcaaaccatc ctccttaccc ctcagcctaa cggcttgcct tacgccggct
     2581 tcgaaagcaa tatcggtgcc ggtcagaaat tcgccagagc cctgccccaa gtcaacatag
     2641 gtggtctcag ccccaacccc gccgtcagac cggtaggagc accaggccgg accggttcat
     2701 cgcgcgccct gacaaacacc caggcccgac cggtcggtaa accagtcacg agcagcaatc
     2761 aggccagcca atatcagccc tatacaccgg gcaatgcagc gggaagcagc ataaacagct
     2821 actcttcctc agcagtatca aaagtatccc tgaccggcag attgctccga aaatagaaag
     2881 cagtctttat caagtagaaa gtattttgca atagccaagt agaacaagct gaaaccctta
     2941 tcatgagcct taattagccc agggtaattt gcagatgcca acagaggcaa ctattaacgc
     3001 cagcaaatgt gataccagac taaccttatc agacgcatat ttatcagcac cacttcccat
     3061 acagaaagct gcattagacg ccgttataca tcaaactcgg tattatcagg caccgcccat
     3121 ggcactacgc ccgagtttat atctgcgcca ccacaaccaa caccacggtt gcatatacct
     3181 cacgcatatc atttatcgga tatcagtatt catgggcatt tccgggcacg ccaagaggac
     3241 gctccgggta ttgccacact atatatgggg tctgccctgg tgtaaggata cccccctgct
     3301 ttcggatttt at
//
LOCUS       DAOQNZ010000276         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14344_length_3308_cov_3.825699, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000276 DAOQNZ010000000
VERSION     DAOQNZ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14344_length_3308_cov_3.825699"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKW73_06265"
     CDS             <1..785
                     /locus_tag="PKW73_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72920.1"
                     /translation="ALTSFSPPGRVSRAGASDYMKLALLIAVLALVSIFGVTAYDAIK
                     DITCVAGDTAVRKARAYLSKGEDENAINILSRALEEDKSGRHKESIQGLLDQSLYERG
                     LKLVQAGQYRDAVTCFSRISKDYVGREEVNKLIADNSDKALPREFSQVMLQTAEAGSR
                     DRREELTRMDKAVMTVVPSTAHLERAGKPVVETSGGRPSVKLLAPVENGVDVSRLQQM
                     ARYNELLAGFFKRRTGPSAQVAQTSAEPPTYEEWVQGGSKEF"
     gene            815..3124
                     /locus_tag="PKW73_06270"
     CDS             815..3124
                     /locus_tag="PKW73_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72921.1"
                     /translation="MIFRRDKKPPKTDAVNASWRVRIQPAIYAAILLAVVFLSHAATL
                     WNGFVLDDKFNVRPFGAVSIAKEKWEPVWYEIYTDAFQKPLSEPLQRLSLVIDYQSGH
                     LNSPGVYHATNLLLAAFGVLLAFFLLNKLCDLLSAHGHKRISYALPLFACALFAAHPL
                     GSESVAYISGRSPLICFVIYMGTCLLFLKGFTARTVGGAVCFSLTSYLGIVLCLFSSC
                     QALTLPWTILILALMTRPKNSTWKDFLYERSYELGSMLLVALALPFVLLFSNQVPELY
                     LSGVPVPDAFPYYCSQLKALVIYYLRVFFLPFGLSIHPNWLTSETSPDLLAALGGLLL
                     AAACGGLYILRRRPLSLLGLTCFLFGLVPSLFPSGIAAHGARFYFSCFGLCLLLSDLV
                     FHLCRHFKLDQVELAHRGYSRKAAIYMALPLLVLAGLSNWRDHAYAGNESLIGGSIRL
                     NKDDSYMQALLSLHLTLTGGDKLERGMKEAEAAIRKNQELALGYLAMAYFNEAKRSYD
                     SSLFHFERALLLTQKYRLGDRLAGFARGGIARSMAAMDMLSTANEVKKVHDFAKEGIK
                     YEPTRARNYLALGRALFAEDKPESAELACRQYDKGRRFDLNDPDFARPVAEAALKTGY
                     RERMEQAYGSAKLVNRLNNNAYTRLLYARAALESGRLHKGFAIMSDYCDNTPKPSAEA
                     LLLLSALEKQSNRKALSDWYLAGARALDKDIEKKVRLHLVVPPKPTRDKDNEGPERLS
                     PTKARVKPDGALELPSTSPPAPSPTPSHR"
     gene            complement(3081..>3308)
                     /locus_tag="PKW73_06275"
     CDS             complement(3081..>3308)
                     /locus_tag="PKW73_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72922.1"
                     /translation="LPEVPQEEELAPLFQIYKNYSPGKVVAVGREKEVTQFLFKDRPA
                     LGKTTIYICRNFSCQKPISDEKELEKELEDW"
BASE COUNT          667 a          917 c          855 g          869 t
ORIGIN      
        1 ccgctcttac gtcgttttcg ccgccagggc gggtgagccg ggccggtgca tccgactata
       61 tgaaattggc gcttcttata gccgttttag ccctggtatc aatcttcggc gtcaccgcct
      121 acgatgccat caaggacatc acttgcgtgg ccggtgatac cgctgtgagg aaggcgagag
      181 cctatctcag caaaggtgag gatgagaatg ccatcaacat tctttcccgc gccctggagg
      241 aagataagtc gggacgtcat aaggaatcca tacagggtct cttagaccag agcctttacg
      301 agcgcggact caaacttgtg caggccgggc aataccgtga tgccgtcacc tgcttcagtc
      361 gtatatcgaa agactatgtg gggcgagagg aagtcaataa gcttatcgcc gataattcgg
      421 ataaagcctt gcccagggag ttttcccagg tgatgttgca gaccgccgag gcgggcagcc
      481 gagaccgccg ggaagaactg acccgcatgg ataaggctgt tatgactgtg gtgccttcca
      541 ccgctcatct ggaacgtgcc ggtaaacctg tggttgagac tagtgggggc aggccttcag
      601 ttaagttgct cgcccctgtg gaaaatggcg tcgatgtatc tcgcttgcaa cagatggctc
      661 gttacaacga gcttctggcc ggtttcttca agcggcgtac cggtccctcc gcccaagttg
      721 cccagaccag tgccgagcct cccacctatg aagagtgggt gcagggggga agcaaagaat
      781 tttgatgggc cctcatgctg aactggcaaa ctaaatgatt tttcgccgcg acaagaagcc
      841 gcctaaaacc gatgccgtca atgccagctg gagggttcgc attcaaccgg ccatttatgc
      901 tgccatcctt ttggcagtag tttttctaag ccatgccgct accctctgga acggttttgt
      961 actggacgac aaattcaatg tcagaccctt tggggcagtc tcaattgcca aagaaaagtg
     1021 ggaaccggtc tggtatgaaa tctatactga tgcctttcag aagcctctct cggagcctct
     1081 gcagcgactg tctctggtca tcgactatca aagcgggcat ttgaattcgc ccggtgtcta
     1141 tcacgctacc aatctcctgc ttgcggcctt tggtgtgctt cttgcttttt tcttgctcaa
     1201 caagctctgt gatttgcttt ccgcccacgg gcataagcgc atttcctatg ccttgccgct
     1261 ctttgcctgt gccctcttcg ccgcccatcc cctgggcagc gagtcggtgg cgtatatttc
     1321 aggacgttca ccgttaatct gttttgttat ctatatgggc acctgtttgc tctttctcaa
     1381 aggttttaca gctcgcaccg taggtggtgc cgtgtgtttc agcctgacca gctatctggg
     1441 cattgtgctt tgcctctttt cctcctgcca ggcccttact ctgccctgga ccattttaat
     1501 tctggctctt atgaccagac ccaaaaactc cacctggaaa gactttctct acgagcgcag
     1561 ctacgaactg ggcagtatgc ttctggtggc actagcactg ccgtttgtcc ttctgttttc
     1621 caatcaagtg ccggaactct atttgagcgg cgtgcctgtt cccgatgcgt ttccctatta
     1681 ttgcagccag ttgaaagcgc tggttatcta ttacctgcgc gtcttcttct taccctttgg
     1741 tctttccatt catccgaact ggctcacctc agagacaagt cctgatctgc ttgccgctct
     1801 cgggggcttg ctcctggctg cagcctgtgg cggtctttac atactgcgcc gaagaccttt
     1861 aagtcttctt ggtttgacct gcttcctttt cggcctggtg ccttcgcttt tcccatccgg
     1921 tattgccgct cacggtgcgc gtttctattt ctcctgtttt ggtctttgcc tgctcctgag
     1981 tgatctggtc tttcacctct gccgccactt caagcttgat caagtggaac tggctcatcg
     2041 cggttacagc cgtaaggccg ctatttatat ggctttgcct cttttagtgc tggctggtct
     2101 ttccaactgg cgcgaccacg cttatgccgg caatgagagt ttgattggcg gtagcattcg
     2161 cctcaacaaa gatgattcct acatgcaggc cttattgtct ctgcatctga ccctgaccgg
     2221 tggcgacaag ttggagcggg ggatgaagga agccgaagcg gccattagaa agaatcagga
     2281 actggctctt ggatatctgg ctatggccta ttttaacgag gctaagcgca gctacgattc
     2341 cagtcttttt cactttgagc gcgctctttt acttacccaa aagtatcggc tgggcgatcg
     2401 tctggccggc tttgcgcgcg gcggtattgc tcgttctatg gcggccatgg atatgctgag
     2461 caccgctaac gaagtgaaga aagtacatga ttttgccaag gaaggcatca agtatgagcc
     2521 caccagggcc aggaattatc tggccctggg ccgggccctc tttgccgagg acaagccgga
     2581 gtctgccgaa ctggcctgtc gccagtacga caaagggcgg cgtttcgatt tgaatgatcc
     2641 cgattttgcc agaccggtgg ccgaagcggc cctcaagacc ggatacagag agcgcatgga
     2701 gcaagcttac ggctcagcca agttggtcaa tcggctcaac aacaacgcct acacaagatt
     2761 actttatgcc cgtgccgctc ttgaatcggg gcgcttgcac aagggctttg ccatcatgtc
     2821 cgactattgc gacaacacgc ccaagccttc agcggaagcc ctcttactgc tctcggccct
     2881 ggaaaagcaa agcaacagaa aggctctctc ggactggtat ttagccggtg ccagggccct
     2941 tgataaagac atcgagaaga aagtaaggct gcacctggtg gtgccgccca aacccacccg
     3001 tgataaagac aacgagggac cggagcgttt gagtcccact aaagccaggg tgaagcccga
     3061 tggtgctctg gaattgccct ctaccagtcc tccagctcct tctccaactc cttctcatcg
     3121 ctgattggtt tttgacagct gaagttccgg caaatatata ttgtggtttt gcccagtgcc
     3181 ggtctgtctt taaagagaaa ctgcgtcact tccttttccc gtcctaccgc tactaccttg
     3241 cccggactat agttcttgta gatttggaag agtggcgcca actcctcttc ctgtggcacc
     3301 tccggtag
//
LOCUS       DAOQNZ010000277         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14346_length_3308_cov_3.568706, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000277 DAOQNZ010000000
VERSION     DAOQNZ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14346_length_3308_cov_3.568706"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKW73_06280"
     CDS             <1..335
                     /locus_tag="PKW73_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72923.1"
                     /translation="YLTEKCLQIVIGGGVGEVAHEKLVIHIYYLMYFLPGSSKRSSAD
                     FCNSGPCIACTYGWDLRSILVLYPGLNRLCFSNRAQKFVQRNCKMRTWPSIQPFNDRT
                     ELHLFGKV"
     gene            615..2540
                     /locus_tag="PKW73_06285"
     CDS             615..2540
                     /locus_tag="PKW73_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNA72924.1"
                     /translation="MGFALGTGTLALQGLTAALCLSFLLPLAPPPLSMGPVPAALAEG
                     KTLVRFRSFDNSIFAEAKRDNKFVLLDLEAVWCHWCHVMDEKTYGNEAVADLLNSRFI
                     CVKVDQDAQPDLSVKYEDYGWPATIIFDGDGREIVKRSGYINPEKMKALLEAIIKDPS
                     PEEAQPPSASLSPASAAEPNADAESKNRGDSDAKSDAKDGLSETLRQDLLDKHLQGYD
                     AKYGAWGTYQKFLDWDSVEYSMLIGLGKDQSDARSYQERARKTLDGELNLLDPAFGGV
                     YQYSTDGDWKHAHFEKIMQTQAENLRIYALGSILYGDKRYLQAARAIAAYLQEFLCSP
                     QGAFYTSQDADLVPGVHSGGYFQLSKEARLKKGSPRVDKHIYARENGWAICALAHLYG
                     ATGDGAYLDQALKALTWIEANRSLGDGGFSHDQKDASGPYLGDTLAMGRAYLALYGVT
                     GRRNYLQAAERCADFMEAQFRLKNGPGYGSARDSGGPIKPVPLLDENVMLSRFCNLLY
                     RYSGEPRYRSMSADALRYLAQPEAALKRKILVAGILLADAEFRNEPTHITVVGPRGDE
                     LSQMLFAAANKLPLVYKRIEWWDASEGPMPNKDTELPQLSQPAAFACSDGRCSRPAHS
                     IVELTAIFEKLTGRKGK"
     gene            2840..>3308
                     /locus_tag="PKW73_06290"
     CDS             2840..>3308
                     /locus_tag="PKW73_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72925.1"
                     /translation="MLASTRAMRHSAMVLTPNFEELRPVMELAAIWRGLVSRSTRILF
                     AGIVLTALATENSAVQAAQDTATVVGTDTAGGEKAALAKPSRAGKIKRRFDQTYKLWN
                     SELDRYVVDGNVNYGLWQKHQEGLKRFLKQVETLSESEYESFSRHEKLAFWLNV"
BASE COUNT          731 a          853 c          926 g          798 t
ORIGIN      
        1 cctacctgac cgaaaagtgt ttgcaaatcg tcatcggtgg tggagtagga gaggttgccc
       61 acgaaaagct tgttattcat atttattacc ttatgtattt ccttccaggt tcatctaaaa
      121 ggagcagtgc tgatttctgt aactcgggac cctgtattgc ttgcacttac ggttgggatc
      181 taaggagcat tttggtgctt tacccagggc tcaaccggtt gtgcttttcg aaccgtgctc
      241 agaaatttgt gcagcgaaac tgcaagatga gaacttggcc ttcaatacaa cctttcaatg
      301 atcgcacaga attgcattta tttggcaaag tttgaaatta atgtgagtat taagattgcg
      361 gctgctgtcc cgaaagctgg cagggggcac cttcccggca aacggatggg ggtttttcgg
      421 gggctaattc aaattcatca aactcaaaat tcgggttgat tcggccctcg cgtagcggtt
      481 gcagcggctc tcaggtccat gggctagaat atcgatcttc ctaaattgtc ggcatttttt
      541 gccgctgaca tcatctccga ggcgccccca taatgaaatc gattcggagc cgatctctaa
      601 agcggagcct gggtttgggc ttcgcgctcg gtaccggtac cctcgccctg cagggcttga
      661 ctgccgctct ttgcctctcc tttttattgc cgctggcgcc gccgccgctc tcaatggggc
      721 cggtgccggc tgcgctggct gaaggcaaga ccctggtgcg gtttcgttct tttgacaaca
      781 gtatctttgc cgaagccaaa cgagacaata aatttgtttt gctcgatctt gaggctgtct
      841 ggtgccactg gtgccatgtg atggatgaga agacctatgg caatgaagcg gtggccgact
      901 tgctcaattc ccgcttcatt tgtgtcaagg tggatcagga tgctcagcct gacctatctg
      961 taaagtacga ggattatggc tggccggcca ccattatttt cgacggggat ggtcgggaga
     1021 tagtaaagcg ctcgggctat atcaatcctg aaaagatgaa ggctctgctg gaagccatta
     1081 tcaaggaccc gagccccgag gaagcacaac cgccgtccgc aagtctcagt cctgcttccg
     1141 ctgcggaacc taatgccgat gccgagtcaa agaatcgggg cgattccgat gctaagagcg
     1201 atgccaaaga cggtctttct gagaccttga gacaggatct tctggacaag cacttgcaag
     1261 gctatgacgc caaatacggt gcctggggca cctaccagaa attcctggac tgggacagtg
     1321 tggagtattc catgctgatt gggctgggga aagaccagtc tgatgccagg tcttaccagg
     1381 aacgtgcacg caaaaccctg gacggggaac tgaatttgct cgatccggct tttggcggcg
     1441 tttaccagta ttccaccgac ggagactgga agcatgccca ttttgagaaa attatgcaga
     1501 cccaggctga gaatctgcgc atctacgccc tcggttccat cctgtacggt gataaacgct
     1561 acctgcaggc ggcacgagcc atagccgctt atctgcaaga gtttttatgc tccccgcagg
     1621 gtgcgttcta tactagtcag gatgcggatc tggttcccgg cgttcacagc ggcggctatt
     1681 ttcaactgag caaggaggcg cgcctgaaaa aaggttcgcc ccgtgtcgat aaacacattt
     1741 atgcccggga gaatggctgg gccatttgcg ctcttgctca cctttacgga gccacaggcg
     1801 atggagctta tctggaccag gctctgaaag cgctcacctg gatcgaagcg aaccgcagcc
     1861 tcggtgacgg cggtttcagc cacgatcaaa aggatgcctc cggaccctac ctgggtgaca
     1921 ctctggccat ggggcgggct tatctggctc tctacggtgt caccggtcgg cgtaattatt
     1981 tgcaggcggc cgagcgctgc gctgatttca tggaagctca gtttcgtctc aagaacggcc
     2041 cgggttacgg cagcgcccga gacagcggcg gccccattaa gcccgtaccc ttgctggatg
     2101 agaacgtgat gctgagccgt ttttgcaatc tgctctaccg ttacagcggg gagcctcgtt
     2161 accgttccat gagtgccgat gccctgcgct atctggctca accggaggcc gccttgaagc
     2221 ggaagattct ggtagccggt attttgctcg ccgatgccga gtttagaaac gagcccacgc
     2281 atattacagt ggtgggtcct cggggcgatg aactgagtca gatgctcttt gctgccgcca
     2341 acaaattgcc tctggtttac aagcgaatcg agtggtggga cgcttcggaa ggtcccatgc
     2401 ccaataaaga cactgagttg ccgcaactgt cacaaccggc tgcctttgct tgcagtgatg
     2461 ggcgctgctc tcgaccggcc cattcgattg tggaattgac ggcgattttt gagaaactga
     2521 ccgggcgtaa gggtaaataa ctcagtttgc tcccctggct ttcctgtcgg cactctccgg
     2581 cagacccggc gcttcaggca agttagactc atctatgaga caattatcgg ccagttaatg
     2641 gaaagggaat gtcccatgga tgatgcgacc ccagttatcg ccgaccggaa gccctgcatt
     2701 ctagagctgg agccgggtga gtattactac tgcacctgcg gccgttcaaa ggggcaaccc
     2761 ttttgcgacg ggtcccatgc cggtactagt ttctctcctc ttcagtttac ggtgactgag
     2821 aaaaagcgga tggcgctttg tgcttgcaag cacacgggcc atgcgccaca gtgcgatggt
     2881 actcactcca aactttgagg agttgcgacc ggtgatggaa ctagcagcta tatggcgagg
     2941 gcttgtctct agaagtacca ggatactctt tgccggtatc gtattgaccg ctctcgccac
     3001 tgaaaacagt gccgtccagg ctgcccagga tactgccacg gttgtcggta ctgatactgc
     3061 cggcggtgaa aaggcggctt tggcgaagcc gagccgtgcg ggtaaaataa aacgtcgctt
     3121 cgaccaaact tataagctct ggaacagcga gctagaccgc tatgtggttg acggcaacgt
     3181 aaactatgga ctctggcaga agcatcaaga agggctgaag cgctttctca aacaagtgga
     3241 gacgctttct gaaagtgagt atgaaagctt cagtcgccat gagaagttag cattctggct
     3301 caatgtct
//
LOCUS       DAOQNZ010000278         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14361_length_3306_cov_3.615503, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000278 DAOQNZ010000000
VERSION     DAOQNZ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14361_length_3306_cov_3.615503"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1986
                     /locus_tag="PKW73_06295"
     CDS             94..1986
                     /locus_tag="PKW73_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72926.1"
                     /translation="MTSKDLRSKTSSKFRPAYVASAFSLVLSGVSAVALADSAFAAKP
                     FQVRITREVVRADQLMLQGKYDEAADLYKGEMGRQPKSVAATVGYGMALAKQFKLDAA
                     EEHFNKVLSMDPTNAMAHAGRAMITLNRLTSSSNTVIKNREALLRNAEAECKQALNID
                     PAMPEGHVTLGNIYKEQGRMDEAINEYKEAIRLDPKYSEGYSQLGIAQLNKEQYADAV
                     SAFKSAISYNSGNSQAHYGLGRAYYKQGLLDEALKELNTSLYQFRNSAPVHQTLGDVY
                     TAQGNTVAGIQQYQESIRIKPENPDCYLRIADIREGRGDLEMSIAELRSGLEMMPNNP
                     DLHLRVADQSLKLEKLDDAIKEYKTVLDANPQNSPAAKGLTRAYYLKANKEATGAFFV
                     SNEFESAQRMIDQAVRMNPNDMELRLAQAKLRSMAGVPVDLASIGTPTNDGERIAYAE
                     ALLAQNNFKDEDSQMNIVISNAADAKQTFAVADLALMIKDLKNAEAAYKKASSYPGAE
                     ERAKRGLNLVMKAKDTARQDLTLADDLARKNQLPSAIDKYHASIYGNPKVADTRLGIG
                     KALERSRPETSKDLKEAVVQYKAFMALSPDMPPKEVEKMNKKIENLSNKAYKLEMKEK
                     ERARGI"
     gene            2034..>3306
                     /gene="murD"
                     /locus_tag="PKW73_06300"
     CDS             2034..>3306
                     /gene="murD"
                     /locus_tag="PKW73_06300"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNA72927.1"
                     /translation="MAEWKNRRVSILGVGRSGVATAAYLTARGAQVLVSESQPKEKVK
                     PDLLASLEKLTAGKTSVECEFGGHTERAVSWPEIIITSPGIPPRADVIVRARALRKEV
                     ISDVELAYRETRGDADTKPLPIIAITGTNGKSTTTSLISYILEFAGRNAPACGNIGVP
                     VLSLLDGKPDFLVMEVSSYQLEYAPTFAPHIGVWLNLTPDHVDWHGNLDAYIEAKRSM
                     FKHQSLSDYAVLNMDDSVVASTINAGEFFPFSVLGECERAIQSAYMQDGFFCANFGSR
                     SRVVCHESEVKIIGRHNQENCLAAISAALLAGLSEKEIKAGLTTFTALEHRLEYVATI
                     DGVPYYNDSKATNTDSTIKALESFPNNKVVLIAGGKDKGTSLNELVQVVKRYASGVIL
                     IGEARERFERALRNGGFNDVYPVATLEEAVNL"
BASE COUNT          859 a          891 c          826 g          730 t
ORIGIN      
        1 taactcactg cttctgaaag acatctatat aagtagagtt tttccggaag cggttgccgg
       61 tagccaatag taaacccagt gatggagaaa tttatgacca gtaaagatct gcgcagtaag
      121 acaagttcca aattcagacc tgcgtatgtg gccagtgcct tcagtctcgt cctttccggt
      181 gtttccgccg tggctttagc cgattctgct tttgctgcca aaccgtttca ggtgcggatc
      241 accagagaag tagtaagagc cgatcagttg atgctgcaag gcaaatacga tgaagctgcc
      301 gatctctata aaggcgagat gggccgtcaa ccgaagagcg tggctgccac cgtcggctac
      361 ggaatggctc tggctaagca gttcaaactc gatgccgcgg aagagcattt caacaaagtc
      421 ctctccatgg atccgaccaa cgctatggcc catgcaggta gagccatgat caccctgaac
      481 aggctcactt cctcttccaa tacggtcatc aaaaaccggg aagcactttt gcgcaacgcc
      541 gaagctgagt gcaaacaagc tttgaacata gatccggcca tgccggaagg tcatgtcacc
      601 ctgggtaata tctataaaga acagggtcgc atggatgaag cgatcaatga gtacaaggaa
      661 gcgattcgcc tcgatcccaa atactccgaa ggctattctc agctcggtat agcccagctc
      721 aataaagaac aatatgccga cgccgtttct gccttcaaga gcgccatttc atacaacagc
      781 ggcaactctc aagctcacta cggactcggt cgtgcatatt acaagcaagg tttgcttgat
      841 gaggccctga aggagttgaa tacctccctc tatcaattcc gtaattccgc tcccgtgcac
      901 cagactcttg gtgatgtcta caccgcccag ggcaacaccg tggccggtat ccagcagtat
      961 caggaatcaa tccgcatcaa gccggagaat ccagattgct acctgcgcat cgccgatatc
     1021 cgcgaaggtc gcggcgatct ggagatgtcc attgccgagt tgcgctccgg tctggagatg
     1081 atgcccaaca accccgatct gcatttgcga gttgccgatc agagtttgaa actggagaaa
     1141 ctggatgatg ccatcaaaga gtacaaaacc gtactcgatg ccaatccgca aaactccccg
     1201 gcggcaaaag gcctgacccg cgcctattac ttgaaggcca acaaagaagc caccggtgct
     1261 ttctttgttt ccaacgagtt tgaatcagcc cagcgcatga tcgatcaggc tgtgcgcatg
     1321 aaccccaacg acatggaatt gcgtctggct caagctaaac tgcgctccat ggccggcgtt
     1381 cctgttgatc tggccagtat cggtacgccc accaatgatg gtgagcgtat tgcctatgcg
     1441 gaagcgctgc ttgctcaaaa taacttcaaa gatgaagact ctcagatgaa tatcgtcata
     1501 tcaaatgccg ccgacgccaa acaaaccttt gccgttgccg acctggcttt gatgatcaaa
     1561 gatctgaaga acgctgaagc agcctacaaa aaagcatctt cttatccggg tgcggaagag
     1621 cgggctaagc gcggcttaaa tctagttatg aaagccaaag atactgcccg tcaggacctc
     1681 accctggctg acgacctggc tcgcaagaac cagttgccca gcgctataga taaatatcat
     1741 gcttctatat acggcaaccc caaggtggcc gataccagat tgggtattgg taaggctctg
     1801 gagcgttcgc gccccgagac ttccaaagac ctgaaagaag cagtagtgca gtacaaggct
     1861 ttcatggctc tttcaccgga catgcctcct aaagaagttg agaagatgaa caagaaaatt
     1921 gagaatctct caaacaaggc atacaagctg gaaatgaaag agaaagagcg ggccagaggt
     1981 atttaaaggc ttctccttct tttcaagccg cattcacctg ttacaatttg cctgtggcag
     2041 agtggaaaaa tcgacgagtc agtatccttg gagttgggcg aagtggcgta gccactgccg
     2101 cctatctgac ggcgcgtggc gcccaggtgc tggtctccga atcacagccg aaagagaagg
     2161 ttaaaccgga cttactggcc agcctggaaa agctgactgc aggaaaaacg tcggttgaat
     2221 gcgagtttgg tggtcatacc gagcgcgccg tcagctggcc tgaaataatt atcaccagcc
     2281 cgggtattcc accccgggct gacgtgattg tcagggcccg tgctctgcgc aaagaagtaa
     2341 tatccgatgt ggaactggct tatcgggaaa cccgcggcga cgccgacacc aaaccgcttc
     2401 ccattattgc cataaccggc acaaacggta agtccactac caccagtttg atttcttata
     2461 tcctcgaatt tgccgggcgc aacgcgccgg cttgcggcaa cattggtgtg cctgttttga
     2521 gcttgctcga cggcaaaccc gacttcctgg tcatggaagt cagttcctac caactggagt
     2581 acgctcccac cttcgcgcct catatcggcg tctggctcaa cctcacccca gaccatgtgg
     2641 actggcacgg caacctcgat gcctatatcg aggccaagcg ttccatgttc aaacaccaga
     2701 gcctcagcga ttatgccgtc ctcaatatgg atgactcagt ggtggcatcc accatcaatg
     2761 ccggcgaatt cttccctttc tccgtactgg gtgagtgtga gcgcgctata cagagtgcat
     2821 atatgcagga tggattcttc tgcgccaact tcggcagtcg tagccgtgtt gtctgccatg
     2881 aaagcgaagt caaaatcatc ggcagacaca atcaggaaaa ctgcctggct gcaatttccg
     2941 cggcgctttt ggccgggctc tccgagaagg aaatcaaagc cggtctgacc accttcacgg
     3001 ctctcgagca ccgcctggag tatgttgcca ctatcgacgg tgtaccttac tataacgatt
     3061 ctaaggccac caataccgac tccaccatca aagctctgga atccttcccg aacaacaaag
     3121 tcgttctgat tgccggtggc aaagacaaag gtacctccct gaatgaattg gttcaggtcg
     3181 tcaaaaggta cgccagcggt gtaatcttga taggcgaagc gcgagaaaga tttgaaagag
     3241 ccttgcgcaa cggtggtttt aacgatgtat atccggttgc taccctggag gaagcagtta
     3301 acctgg
//
LOCUS       DAOQNZ010000279         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14436_length_3296_cov_3.059550, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000279 DAOQNZ010000000
VERSION     DAOQNZ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14436_length_3296_cov_3.059550"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PKW73_06305"
     CDS             complement(<1..938)
                     /locus_tag="PKW73_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72928.1"
                     /translation="MSFVPKATTTIIMIKRIMSKRRNSLPKLLPIFSLLVLMLVFAEP
                     ALATKLSPALTRDVKTVFGPETKIRLDGSLETKSGEHYLPALPPGFNTRTAPGKVLSV
                     HPARGLPDLVVFEGGFCYVRVIKRPGLRTLPPLSGLDEKSRKYLTACRLVADLIVPEE
                     FVLAGEYKPLVGDLAITVGQEPPASPLTTHVKDDHKKSLGGIYVTSPATGSIILVDEK
                     SLKKSSEFPTEGTPLGMACVGNRLYIADQSKARLLIIDTFKREFVKRQIDLPPGSAPK
                     AVAPLPSGQLLYVTESATGLVDCIETDTGKVLVRTKV"
     gene            complement(868..2175)
                     /gene="dnaJ"
                     /locus_tag="PKW73_06310"
     CDS             complement(868..2175)
                     /gene="dnaJ"
                     /locus_tag="PKW73_06310"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNA72929.1"
                     /translation="MTKRDYYEVLGVSRSASGDEIKKSFRNRARQLHPDNKESGDEAA
                     FKELAEAYEVLSDEQKKAAYDRYGHEGVKGSTRDFDNVDFSSFQGFGFEDIIDALFGG
                     GGGFRSGGFSSSRTGPRQGAHLKYDLEIEFLDAVFGVDKKVSVKRLEDCASCEGSGAA
                     PGSQLNTCATCNGQGQVRQMVNMLFMQSYQVISCPDCSGIGKKIDKPCKDCKGAGQTR
                     KTREFDVKVPAGIDSGTRLRLSQAGDKGARGGPYGDLFVIISVREHKQFIRDGQTIHL
                     RQNLSFSQAALGCELKVPTVEGTKSLKIPSGIQSGSQLAMPGLGVPQIGNPNRRGEQI
                     VHIFVETPTKLSSEEKKLFERLAQLRGEKLSAEADKEGDKPESKTEENGRKDKKKNGK
                     KEDGHGHKPKEEENIFEKIVDVFRPKGDHDHHHDQENHEQEKK"
     gene            complement(2332..>3296)
                     /locus_tag="PKW73_06315"
     CDS             complement(2332..>3296)
                     /locus_tag="PKW73_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNA72930.1"
                     /translation="EDIESIILVGGSTRIPAVQDMIRRFFQKEPSKSVNPDEAVALGA
                     AIQASILAGDVQDVLLLDVIPLSLGIETAGGLFTRIIERNTTIPTSRTMTFTTTEDGQ
                     TSVEVHALQGERDIAAANKSLAKFHLTGIPPAPRGIPKIEVTFDIDADGIVHCSARDF
                     GSGIKHSVTIQRSTGLSPEEVESYKREAEEFAEQDKAVKERIAARVQAESLCAEAERT
                     VTKYGERVEKVHVDKVLRAVEAVKEALETDNDEGLRPLIAGLDVSLLDLGRAIHSGNR
                     KVDKAPESPRSTVKEKSMKSEPLVESFAGEDEDVPELDLDLSKD"
BASE COUNT          714 a          938 c          834 g          810 t
ORIGIN      
        1 accttggtgc gcaccagcac cttacccgtg tccgtttcaa tgcaatccac cagaccggtg
       61 gcgctttcag ttacatatag caattgcccg ctcggcagtg gcgccaccgc ttttggtgca
      121 gaacccggcg gcagatcaat ttgtcttttt acaaattcgc gcttgaaagt atctatgatg
      181 agcaagcgcg ctttactctg atcggcgata taaagcctgt ttcccacgca agccataccc
      241 agaggagtgc cttccgtggg gaactcactg ctcttcttga gactcttctc gtcgaccaag
      301 atgatggacc cggtggcggg agaagtaaca taaatgccgc ccaggctctt tttgtgatca
      361 tcttttacat gcgtagtaag tggactggcc gggggctcct gcccgacagt aatggccagg
      421 tctcccacca gaggcttata ctcgccggca agtacaaatt cttccggcac gatcaagtca
      481 gccaccaagc ggcatgcggt tagatacttg cgcgactttt catccaggcc ggataggggc
      541 ggcagggttc tcagacccgg tcttttgatg acgcgcacat aacaaaagcc gccctcgaat
      601 accactaaat cgggcagacc gcgggcagga tggacggaca gtactttgcc gggggcggtg
      661 cgagtattga agcctggcgg tagagccggc aaatagtgct ctccgctctt ggtctccagt
      721 gagccgtcca ggcggatctt ggtctcaggc ccgaagacgg tcttaacatc tctggtcaaa
      781 gccggggaga gcttagtggc cagcgccggc tccgcaaaaa ccagcattag caccagcaaa
      841 gaaaagatgg gaagcagctt tgggaggcta tttcttctct tgctcatgat tctcttgatc
      901 atgatgatgg tcgtggtcgc ctttgggacg aaagacatcg acaatcttct cgaagatgtt
      961 ttcttcttct ttaggcttat gaccatgtcc atcttctttt ttgccgtttt tcttcttgtc
     1021 tttgcgaccg ttttcttcgg tcttactttc cggcttgtcg ccttccttat cagcctcggc
     1081 tgaaagtttc tcaccacgca actgcgccag tctttcaaaa agtttctttt cttccgaact
     1141 gagcttggtc ggtgtctcga cgaagatgtg cacaatttgc tcgccgcggc gattgggatt
     1201 gcctatctgc ggcacgccca gcccgggcat ggccaactga ctgcctgact gaattcccga
     1261 cgggatcttc aatgactttg ttccctctac ggtgggaacc ttgagttcac agccaagagc
     1321 ggcctgagaa aaactcaagt tctgccgcaa gtgaatggtc tgaccatctc tgataaattg
     1381 cttgtgttcc ctgaccgaga tgatgacgaa aaggtcacca tagggaccgc cgcgagcccc
     1441 cttgtcaccg gcctgcgaaa ggcgcaatct ggtaccgcta tcgataccgg cgggaacctt
     1501 cacatcgaat tctctggtct ttctggtctg cccggcgccc ttacaatctt tgcaaggctt
     1561 atcaattttc ttaccgatgc cggaacaatc aggacaagaa ataacctgat agctctgcat
     1621 gaaaagcatg tttaccatct gacgaacctg gccctgacca ttgcaggtgg cacaggtatt
     1681 taactgactg cccggagccg caccggaacc ttcgcaactg gcacagtctt ccaggcgctt
     1741 gacgcttact ttcttgtcta cgccgaagac ggcatcgaga aactcgattt ccaggtcata
     1801 cttgagatgc gccccctgcc tcggaccagt gcgcgaagag gagaagccgc cgcttctaaa
     1861 gccgccgccg ccgccaaaga gagcgtcgat aatgtcttca aaaccaaagc cctggaagga
     1921 gctgaaatcg acgttgtcga agtctcgagt ggagcctttg acaccctcat gaccataacg
     1981 gtcataagcc gctttcttct gctcgtccga gagcacctca taagcttcgg ccagctcctt
     2041 gaaagcagct tcatcgccgc tttccttatt gtccgggtgc aattgccggg cgcggttgcg
     2101 aaaagacttc ttaatctcat cgccgctggc actgcggctg acaccaagaa cttcgtagta
     2161 atcgcgttta gtcatagtat ccaagagacc ttgctccatg ttgatccgct ctgtatttta
     2221 ttaaaagtca agagccgcaa ccagtcccat aatgtttgta ttgctttaat gaagttgaag
     2281 ggagccgcca cggctcctgt tgcatcgggc ttacgttacc tttgtgaagc gtcagtcctt
     2341 gcttaagtcc agatccagtt ccggcacatc ctcatcctcc ccggcaaagg actccaccag
     2401 aggctcactc ttcatggatt tttccttaac tgtcgaccga ggagactccg gcgccttatc
     2461 tactttgcgg ttgccggagt gaatggcacg acccaaatcc agcaaagaca catcaagacc
     2521 ggcaatcagg gggcgcaaac cttcatcatt atcggtttca agggcttcct tcaccgcttc
     2581 cacggcccgc aagaccttat ctacatggac cttttccact cgctcgccgt acttggtgac
     2641 ggtgcgctcc gcttccgcac agagggattc agcctgaacc cgggcggcaa ttctttcctt
     2701 aacggctttg tcctgttcgg caaactcttc cgcttcccgc ttgtaagact ccacttcctc
     2761 ggggctcaaa cctgtggagc gctgaatcgt aaccgaatgc ttgatgccgg aaccaaagtc
     2821 acgagcggaa cagtgcacga tgccgtcggc atcgatgtcg aaggtcactt cgattttcgg
     2881 aatgcccctg ggtgccggcg gaatgcctgt taaatggaac ttagccaggc tcttattagc
     2941 ggccgcaata tccctttccc cttgcagggc atgcacttcc accgaggtct gaccatcttc
     3001 cgtggtggta aaggtcatgg tccggctcgt ggggatggtc gtgttccgtt cgatgatgcg
     3061 agtgaagaga ccgcccgctg tctcgattcc aagtgaaagg gggatgacgt ccagaagcaa
     3121 aacatcttgc acatcgccgg ccagaatgga agcctggatg gcggcaccta aggctacggc
     3181 ttcgtcggga ttgaccgact tggatggctc cttctgaaag aaacgccgga tcatgtcttg
     3241 tacagccggt attctggtag agccccccac cagaataatg ctctcaatgt cctcgg
//
LOCUS       DAOQNZ010000280         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14442_length_3295_cov_3.925617, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000280 DAOQNZ010000000
VERSION     DAOQNZ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14442_length_3295_cov_3.925617"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKW73_06320"
     CDS             complement(<1..219)
                     /locus_tag="PKW73_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72931.1"
                     /translation="MDFLMGKIFFGRVGSSSSVLSFCYFLAVLFLLALSAQNAGAQGF
                     YNGGARRLPPVNMDSFVQQAGGAAEAIYG"
     gene            360..695
                     /locus_tag="PKW73_06325"
     CDS             360..695
                     /locus_tag="PKW73_06325"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNA72932.1"
                     /translation="MIIYEVNLEVDESIKFKYAGWLPSHIEKMMTMPGFMNAYWFFRE
                     PQFEGKDPKKTLWTIQYFLKDQESLDHYLNGKAKEMRQEALDLFGSEHFKAERRVLYP
                     LAGAGEVNT"
     gene            692..1939
                     /locus_tag="PKW73_06330"
     CDS             692..1939
                     /locus_tag="PKW73_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNA72933.1"
                     /translation="MKKAGRQERAILALLLACLCESASAGAAEAADVFSRLSLSQAKE
                     QAGQDKKLLLLDFTASWCPPCQKMERTTWEDQEVKDWIKQNALAVQIDVDQNSSDTRA
                     LQVEAMPTLILFTPAKGGAEFGRKVGYQSPAELLRWLEGARSGKSKEELEKETMDGSD
                     NAVFEGLGRARQCQTAGQYDEALNEYIRLWQQTAGGNKVLSELRTGLIAHELKQLVKS
                     HPPARARLVDLRKSAEAADNRLDVVMLNGILDENARTLEWFDQVKDKPEARPLIARLS
                     NQLVQVLYSACRWSDAATYLYGDPIKQINEYYKQAEAMKKPAPHTEVSKDFDPFPSMV
                     LMLYGSYVGAGKEAQAQAIKDECLRLDDTQTMHEALDNMAQGMRQARKAAAKSNAAIA
                     GAKPEATKRPPAAPIKGKSGTRH"
     gene            complement(1967..2896)
                     /locus_tag="PKW73_06335"
     CDS             complement(1967..2896)
                     /locus_tag="PKW73_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72934.1"
                     /translation="MRALLLLPLFLLPLSLLFGPRAVPYAMGKPPAGVRLEDWCNKHF
                     LQDPADITHDPVFKQIQRFRKARRIDQALSYTDGLVKSHPTSGNCLRHRAYVYALLTE
                     DDKAMKDLAAACRDKKLTGDDWYAIGRLYSKLDADKEALNAFGQAVRLSSNPYNALRM
                     KAKEELKMNLLDAAEKDLSRCVELKAGSSRNLIDLAKVHTLLKKHTLVIEDVKKAEET
                     GRLNEREEVKAMRMRADAFVALKKWQEALKDLNRGLAIAPLTRDLYARRLKIYELTGN
                     KTAAAKESALLVKLDGSLKSAPGVDEDRPVEAD"
BASE COUNT          850 a          926 c          873 g          646 t
ORIGIN      
        1 accatagatg gcctctgcgg caccgcccgc ttgttgcaca aagctatcca tattgacagg
       61 cggcaggcgt cgggcaccgc cattgtaaaa gccctgggca ccggcgtttt gtgctgacag
      121 agccagcaaa aacagcaccg ccaggaaata gcaaaatgaa agcacgctgc tgctagagcc
      181 gacccggccg aagaaaattt tgcccataag aaaatccatc ctccggacct caggtccgag
      241 tattggtgaa gactttagac cggtctggcg ccggtaccgg tggtagtaat ctgcatcagt
      301 tataatgcct gtcacataaa atttccgtga aatagaaagc gagcaaggtc aagcaaagca
      361 tgattattta cgaagtaaat ctggaagtag atgaaagcat caaattcaaa tatgccggct
      421 ggctgccctc tcatattgag aaaatgatga ctatgcctgg ttttatgaat gcctactggt
      481 tcttccgcga accgcaattc gaaggcaaag atccaaagaa aacgctctgg acaattcagt
      541 attttctaaa agatcaggag agtctcgacc attacctcaa tggcaaagcc aaagagatgc
      601 gccaggaagc tctcgatctt ttcggctccg agcacttcaa ggcagaaagg cgtgtactct
      661 atcccctggc cggtgcaggc gaggtcaaca catgaaaaaa gcggggcgtc aagaaagggc
      721 gatactggct ttacttctag cctgcctgtg cgaaagcgca agcgccggag cggccgaggc
      781 cgccgatgta ttttccaggc tttccctctc tcaagctaaa gaacaggcag gccaggacaa
      841 aaaacttctg cttctggatt tcacggcaag ctggtgtcca ccctgccaga agatggaaag
      901 aaccacctgg gaagaccagg aggtgaaaga ctggatcaag caaaatgctc ttgccgtgca
      961 aatagatgtc gatcaaaaca gcagcgatac ccgggccttg caagtggaag ccatgcccac
     1021 cctgatcctc ttcacaccgg caaaaggcgg agcggaattc gggcgcaagg tcggctacca
     1081 gagcccggcg gagctactgc gctggctgga aggggccaga agcggtaagt caaaagagga
     1141 gctggagaaa gaaaccatgg acggcagcga caatgccgtt ttcgaagggc ttggtcgagc
     1201 caggcagtgc cagaccgccg gtcagtatga cgaagcactt aatgaataca tccggctctg
     1261 gcagcagacg gccggcggca ataaagttct ttccgaactg cgcaccgggc ttatcgccca
     1321 tgagttgaag caacttgtta aaagccaccc gcccgccaga gcccggcttg tggatttacg
     1381 gaagagcgcc gaagcggcgg acaacaggct ggatgtggtg atgctgaacg gcattcttga
     1441 tgagaacgcc cgtactctgg aatggttcga ccaggtaaaa gacaagccgg aagcacgccc
     1501 tttaattgcg cgtctgagca atcaactagt gcaagtgctg tattcggcct gccgctggtc
     1561 cgacgccgcc acctatcttt atggcgatcc catcaagcag atcaacgagt attacaagca
     1621 agcggaagcc atgaagaaac cggcaccgca caccgaagta tccaaagact tcgacccttt
     1681 cccgagcatg gtcctcatgc tttacggctc ctatgtgggg gccggcaaag aagcccaggc
     1741 gcaagcgatc aaagacgagt gcctgcgtct ggatgacacc cagaccatgc atgaagctct
     1801 ggacaacatg gcccagggta tgcgccaggc aagaaaggca gcggccaaga gcaatgccgc
     1861 tatcgccgga gcaaaaccgg aggcaaccaa acgccctccg gcagccccga tcaaaggcaa
     1921 aagtggaacc aggcactgac cctcaagtgc agtccacaaa ttcgcctcaa tccgcctcga
     1981 cgggccggtc ctcatccact cccggagccg atttcaaact gccgtccaat ttgaccagaa
     2041 gcgcgctttc ttttgccgcc gcggtcttat tgcccgtaag ctcatagatt ttcaggcgcc
     2101 tggcatagag atcacgggtg aggggggcaa tggccagccc tctgttcaag tctttcagag
     2161 cctcctgcca ctttttcaaa gccacaaagg cgtcggcgcg catacgcatc gccttaacct
     2221 cctcacgctc attgaggcga ccggtttcct ccgctttctt cacgtcttcg atgacgaggg
     2281 tgtgcttctt gagcagggtg tgtactttcg ccagatcaat gaggttacga ctggagccgg
     2341 ctttcagttc cacacagcgg ctcagatcct tttcagcggc atcgagcaag ttcatcttca
     2401 actcttcctt ggctttcata cgcaaggcat tgtagggatt gcttgaaagc ctgacggcct
     2461 gcccaaaggc attcaaggct tccttgtccg cgtccagctt ggaatagagc cggccgatgg
     2521 cataccagtc atcgcccgtc agcttcttat cccggcaggc ggcagccaga tctttcatag
     2581 ccttatcgtc ttccgtcagc aaggcataca cataagcccg gtgccgcagg caattaccgc
     2641 tggtcgggtg gctcttcacc agtccatctg tgtaagaaag agcctgatca attctgcgcg
     2701 ccttgcggaa acgctgaatt tgcttaaaaa ccggatcatg ggtgatgtcg gcaggatcct
     2761 gcaaaaaatg cttattgcac cagtcttcca ggcggacacc ggcaggcggc ttgcccatag
     2821 catagggtac agcccgcggc ccgaataaaa gagacagcgg caaaagaaac agcggcagaa
     2881 gcagcaaagc tctcaaagca aagccctttt ggaattgccg ctctcgagta gcgcgcggca
     2941 aatggttata gcttgactcc gtttgggccc tggcatagac agcagcggca aggctaaagc
     3001 cattatacag cgccctcagc cccaggcggc gccgtcttca tgcgtacttt cccccttttc
     3061 cagaaaggct gccggcctta ttctcgctct actggtcgcc tatcgaccag gcaaaagagc
     3121 gaatcactaa tgtccgacat tccaccagga caaccgaaaa tacatgggct gattcaaaac
     3181 ctggctacca gccttacgga ttcaatcagc ggcgccaccc agagctggaa aaccaagccc
     3241 tggaacgtac ttggcgtggg acgcaaacca acggatgaag agcgcagact gagcg
//
LOCUS       DAOQNZ010000281         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14450_length_3294_cov_3.726150, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000281 DAOQNZ010000000
VERSION     DAOQNZ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14450_length_3294_cov_3.726150"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKW73_06340"
     CDS             <1..642
                     /locus_tag="PKW73_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNA72935.1"
                     /translation="NQLTKLPQTTVSIVSARSAERLKKDYYSAEAILAGNYGLEVYFP
                     DATAVIQKEALEALPLLRSLRQLIADELESTLPICLDDHVLSLCLHYHLVPEHEQQIV
                     HQKVAAFAKQFTGLQFRALPTSYEVLPPFDWSKGHSLSVIAQRLGLDDGKTCFLFIGD
                     SEADEAAFQWCNEAGGISILVNSRVVEGSAAKYRVHSPGQVLAFLQYLTGSRN"
     gene            complement(668..1042)
                     /locus_tag="PKW73_06345"
     CDS             complement(668..1042)
                     /locus_tag="PKW73_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNA72936.1"
                     /translation="MMKLTGSYLLAGASQTSANELHKLTTHTDKNVRRRVADRKRVGP
                     DLLNRLAEDPSAEVRAAVAANPQTNLGTLLKLADDPNPDVRFSMAEDHNLPKAILKLL
                     ANDKNTLVQWRANMTLEHLWNC"
     gene            1218..2501
                     /locus_tag="PKW73_06350"
     CDS             1218..2501
                     /locus_tag="PKW73_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HNA72937.1"
                     /translation="MAPRFFRHVDETILSRLQQRLEQNGRLSRDFLVLLAGATAIATL
                     GLFQNSPAVIIGAMIIAPLMRPLTCLSLAMITADARQLVKSVLTLLVGSVLGIIISTT
                     MAWLLRALELTPEILARTHPTLLDLGVALAAGAVGAYCQADEKLSDTMAGVAIAVALV
                     PPLSVVGIGLAFDSPSVSFGAALLYATNLVGITIAGSLVFLLQGFSPLKLARRGLAIS
                     GVCILMLGVPLALSMRELILENQISAKVKTVLKEKTVTFRDAQLREVKVTRFKVPMTV
                     KATVLSSGDTITPRQVTLVQDLLIKEIGLPLKFALRIIPATEVTALDVSPGGETRVMT
                     PLYNSADFGSDIDLPAAEQAGPNPEPNSENSGPQEKSATGAPELKTSEPTEVKGSQNK
                     EGLDSVTVDPAAEEADRLAPAPQKESAVEAHGIKK"
     gene            complement(2531..>3294)
                     /locus_tag="PKW73_06355"
     CDS             complement(2531..>3294)
                     /locus_tag="PKW73_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA72938.1"
                     /translation="PATRMAVNSFWEYLAFLVNSLIFLLIGLQVKFSLLLKYGVQIAV
                     GVLVVLLARLVVVYGLTPVIGLVEHKLPWKWRHLLFWGALRGSVCMALALSLPETFPY
                     KEMITITTFGVVLFTLLIPGLTIEPLVTILKMNAKDPKHEKYRTLRAQLITKKKALPF
                     LADQLKTGSITALAHDRLHSELNATKEVLTKQISDLQMEDASIKSLEEEELRHRIYVL
                     EKDSLKELVKDGVLAEESLQKLEIECQYFSDETTQ"
BASE COUNT          750 a          792 c          852 g          900 t
ORIGIN      
        1 aatcaattga ctaaattgcc tcagaccacg gtttcaattg tcagtgctcg cagtgccgag
       61 cgtctcaaaa aagactacta ctcggcagag gctatactgg ccggcaatta tggtttggaa
      121 gtctatttcc ctgatgctac cgccgttatt cagaaggaag cacttgaagc gctgccgctt
      181 ttgcgcagtc tgaggcagct tattgccgat gaactggagt cgactttgcc gatatgcctt
      241 gatgatcacg tgctctcttt gtgtttgcat tatcacctgg tgccggagca cgaacaacag
      301 atcgttcacc agaaggtcgc cgcatttgcc aagcaattta ccggcttgca gttccgggct
      361 ctgcccacca gctacgaagt attaccgcct ttcgattgga gcaagggcca ctcgctctct
      421 gtgattgcac aaagactggg gcttgacgat ggtaaaacgt gctttctgtt tatcggtgat
      481 tccgaagcgg atgaagctgc ctttcagtgg tgcaacgaag ccggcggcat ttcaattctg
      541 gtgaatagcc gagtggtgga gggtagcgct gcaaagtacc gggtgcactc gcccggacaa
      601 gtactggctt tcttgcagta cttgactgga agtcgtaatt aaaagcaaga gcggctctca
      661 ggatggctca gcagttccac aggtgctcta aagtcatatt tgctctccat tggaccaaag
      721 tgtttttgtc gttggctaaa agtttcaata tggctttagg gaggttatga tcttcggcca
      781 tggagaatct cacgtccggg ttaggatcat ctgccagttt aagcagagtt cccagattgg
      841 tttgcggatt ggcggctacc gctgctctta cttccgcgga gggatcctcc gcgagcctat
      901 tgagaaggtc cggtcctact ctttttctat cagcaactct tcgtctgaca tttttatcgg
      961 tatgtgtggt cagcttgtgc aattcattgg cggatgtttg gcttgcgccg gctaataagt
     1021 agcttcctgt taacttcatc atgacttctc ctctggcagc actgagcgac cggcttgaac
     1081 gccggtgggc tgcaacgatg tttctattat agcacaatta gttcgtttga caaacaaacg
     1141 caaaacctgg ggattaagat atgatggatt ccaaacagac tgaacaaagg cctgtgggca
     1201 aggtagcaat aaattaaatg gcgccgcgat tttttcgtca cgttgatgaa acgatattga
     1261 gcaggctcca gcagcggctg gagcagaatg gcaggctctc aagggatttt cttgtgctgc
     1321 ttgccggagc taccgcaatt gccactctcg gtttgtttca gaacagtccg gcagtaatta
     1381 tcggtgccat gataatcgcc cctttgatgc gtccgcttac ttgcctttct ctggctatga
     1441 ttactgccga tgccaggcag cttgtaaagt ctgtgctcac gcttctggtg ggatcagtat
     1501 tgggaataat catttccacg acgatggcct ggctcttgcg cgcgttggaa ctcactccgg
     1561 agatactggc tcgcactcat cccacattgc ttgatcttgg tgtggcgcta gcagctggag
     1621 ctgtcggagc atattgccag gccgatgaga aactgtctga tactatggcc ggtgtggcca
     1681 ttgccgttgc tcttgtaccg cctctcagcg tcgtggggat aggtctggcc tttgattcgc
     1741 cttctgtatc tttcggggcc gccttgcttt atgcgaccaa cctggttggt atcaccatcg
     1801 ccggctcttt agttttcctt ttacagggtt tcagcccgtt gaaactggct cgcagagggc
     1861 tggcaatttc cggcgtctgt atcctgatgc tcggcgtgcc tttagccctg agtatgcgtg
     1921 agctgattct tgagaatcag attagcgcca aagtaaagac ggtcctgaag gagaagacag
     1981 tgacttttcg cgatgctcaa ttgcgtgagg tgaaggtcac tcgcttcaaa gtaccgatga
     2041 ctgtgaaagc tacggtactg agctccggtg acacgattac tccccggcag gtgaccctgg
     2101 tgcaagatct tcttataaag gaaattggac tgcctttgaa gttcgctttg agaatcattc
     2161 ctgctacgga agtgactgct cttgatgtct cgccgggtgg tgaaacgcgt gtcatgacgc
     2221 ctctttacaa ttccgctgac ttcggctcag acattgattt gccggcggct gagcaggcgg
     2281 gtcccaatcc ggaaccgaat agcgagaatt ccggaccgca agaaaaatct gctactggag
     2341 cgccggaatt gaaaacatcg gagcccaccg aagttaaagg ctcgcagaat aaagaaggct
     2401 tggactcggt caccgtcgac cctgctgccg aagaggctga tcggttggcg ccggctcctc
     2461 agaaagaatc agcagtagaa gcgcacggta tcaaaaagta gttgcagttt tggctttgta
     2521 ataccaattc tcactgtgta gtttcatctg aaaagtactg acattcaatt tctagttttt
     2581 gcaggctttc ctctgctaat acgccatctt ttacaagttc tttcagggaa tctttttcca
     2641 gtacataaat tctgtgcctc aattcttctt cttccagtga tttaatggat gcgtcttcca
     2701 tctgtaaatc gcttatctgc tttgtcagga cttcttttgt ggcgttcaat tccgaatgaa
     2761 ggcggtcgtg agccagggct gttatggagc ctgttttaag ttggtctgct aggaaaggca
     2821 gcgctttctt cttggttatg agctgtgctc tcaacgttcg atacttttcg tgcttgggat
     2881 ccttagcatt catttttaga atggtcacaa gcggttctat ggtcagacct ggaatcaata
     2941 gagtaaatag caccacgccg aaggtggtta tagtaatcat ctctttgtac gggaaagtct
     3001 cgggtaggct gagcgccagc gccatacaga cggaacctct gagcgcaccc cagaatagta
     3061 aatgccgcca cttccaggga agcttatgtt ccacaagtcc aataacgggt gtcaagccat
     3121 aaacgacaac caatctggca agcagcacta ccagcacacc tacggcaatc tgcacgccgt
     3181 atttgaggag caaggagaac ttcacctgca gtccgattaa aagaaaaatt agtgagttga
     3241 ccagaaaagc caggtattcc cagaaggaat taaccgccat tcgtgttgcc gggg
//
LOCUS       DAOQNZ010000282         3290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14490_length_3290_cov_4.390108, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000282 DAOQNZ010000000
VERSION     DAOQNZ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14490_length_3290_cov_4.390108"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKW73_06360"
     CDS             complement(<1..234)
                     /locus_tag="PKW73_06360"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HNA72939.1"
                     /translation="MERAIFASGCFWKPQFIFSQIPGVLSTAVGYCGGNVDNPHYRQV
                     CTDTTGHAEAVLVEFDPSVVSYKELLAKFFSIHD"
     gene            complement(326..>1683)
                     /locus_tag="PKW73_06365"
     CDS             complement(326..>1683)
                     /locus_tag="PKW73_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72940.1"
                     /translation="GIYHPSEHASRHLDEGWAVAMGEEIMAPDPDMLFVYASKAPVRT
                     MVLSAALESGIKSGGKPVNPVAEALLRRAQYLDEAVRPYANEVLAERALTGTPAQKGD
                     AIELLTLFGGKEMVGKLREIALDRRSKVVIPPEGTSQTVSHSELPTGFARPANDNSLP
                     GLGGRNMQGRPQERTVQQAAFDAMVKLSGGSATDKIQFALQEGLQHPELRGLAREHVF
                     RQNDGRAPFYRRILEFEGQAFETAPLIKQMANRMFADQFGREMAYSELMRARTNNPSG
                     QIEISAEVLTKVPSLSNKVFDQFEKMMKNGLNPKDLRRINQIMEESTAHLTQTERHRA
                     QEIGSMALRYRLVDDAMRSVSNPQQDQMKALESLAAVKDQKAIKPLLEMAISGRPDVE
                     RQAIAALGHYSPNIIKHFARSMKPGMANDPVRIRRLDQLLQGRYAVSFYAAPDDGSTR
                     H"
     assembly_gap    1684..1783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1784..>3290)
                     /locus_tag="PKW73_06370"
     CDS             complement(<1784..>3290)
                     /locus_tag="PKW73_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72941.1"
                     /translation="KTAFQSSTYKDAEGNFSAGSTITNFAVGTGLGGTLSVPAGFLGL
                     RVGRASSVLLGSAENSVTQTVVKNVVTGAAGGGAGGAVFGGVEAAREKMSISHIMNGV
                     MEGGLIGMGTGALMGGAEKVHRPLSQKSAPHEQAVGKPVDVPETAPGKGLKADGPDAL
                     ARTTSASDAETSVRERRRMTLEERIAYLWSDEGRMQAYEGLSFKPFEAVSIKELQPKL
                     KVVGTESRNEFELKADVSAKHEDLVKLIKPPGAKEASWTEFNKLLEFKPRDHVVYQVD
                     KLSTRISVPQEYAVQLNEVRALRLRAEMPESYRNLPESIKSKLEAQAAQGDARGFAEV
                     LPPNEVTKVVAMFEARKALAVHPLRNRVLPEDVIPLLEALPQSQKITEVTLSDKPSFI
                     DAFNRVEYDSPRFSSAARVGQDGHVIFSQAELNSSFTTYFSHELSHLTKWGSPTFSKL
                     FDKATSVDRVENNVNHVKSRKARESGRVGDVEEPGIYHPSEHASRHLDEGWA"
BASE COUNT          696 a          915 c          837 g          742 t
ORIGIN      
        1 atcgtgaatg gaaaagaact tggccaaaag ttccttatat gagacgacgg acggatcaaa
       61 ctccaccagc accgcctcgg catgtcccgt ggtgtcggtg caaacctgcc tgtaatgggg
      121 attatctaca ttgccgccac aatacccgac agcggtgctg agcacacccg gaatctgact
      181 gaagataaac tgcggcttcc agaagcaacc gctagcaaat attgctcttt ccacgtcgac
      241 ctcctgccgg ggaaatgccc ggttacgaca gcaattttaa ccacactgat aatatacagc
      301 gtccagcacc tgaaagttcc tgatcttaat gcctggttga accatcatcg ggggcggcat
      361 aaaaactaac ggcatagcgc ccctgcaaga gttgatccag cctgcgaatt cgtactggat
      421 cattagccat gccgggtttc atagacctgg caaagtgctt gatgatattg ggagaataat
      481 gaccgagggc cgcaattgcc tgccgctcta catcagggcg accggaaata gccatttcca
      541 gaaggggttt gatggctttc tggtctttga cagcagccag actctcaagg gctttcattt
      601 gatcttgctg gggattgctg accgagcgca tggcatcatc cacaaggcga tacctgaggg
      661 ccatagagcc aatctcttgc gctcgatgac gctcggtttg ggtgagatga gcagtacttt
      721 cttccatgat ctggttaatg cggcgcagat cctttggatt gaggccattt ttcatcatct
      781 tttcaaactg atcaaagacc ttgttactca aagacggcac ttttgtgagc acctcagcgc
      841 ttatctctat ttgccccgag ggattattgg tacgagcgcg catcaattcg gaatatgcca
      901 tttcccgacc aaactggtct gcaaacatgc gattggccat ttgcttaatt agtggtgccg
      961 tctcaaaagc ctgcccttca aattccagaa tgcgacgata aaagggggct ctgccgtcgt
     1021 tttgtctgaa tacatgctcc ctggccaggc ctctcaactc gggatgctgc aaaccttctt
     1081 gcagggcaaa ctgaatcttg tcggtggcgc tgcccccgga gagtttgacc atggcgtcaa
     1141 aggccgcttg ctgcacggtg cgctcctgcg gccgcccctg catattgcga ccgcctaaac
     1201 cgggcaggga attatcattg gccggtctgg caaagcccgt cggcaattcc gaatgactga
     1261 cggtctgact ggtaccctct ggcggaatca ccaccttact gcgacgatcc agggctattt
     1321 ccctcagctt gcccaccatt tccttgccgc cgaatagagt caaaagctct atggcatcac
     1381 ctttctgggc aggcgtacca gtgagcgctc tttcggccag cacctcattg gcatagggac
     1441 gcacggcttc atccagatac tgggcgcgtc tcagcagagc ttcagccaca gggttaaccg
     1501 gcttgccacc agacttgata ccactttcca gggccgcact cagcaccatg gtacgcaccg
     1561 gcgccttaga ggcataaacg aagagcatat cagggtcggg agccataatt tcctcgccca
     1621 tggccacggc ccaaccttca tcgagatggc gggaagcatg ctcggatgga tggtaaatac
     1681 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcccaac cttcatcgag
     1801 atggcgggaa gcatgctcgg atggatggta aataccgggc tcctccacat cgcccacacg
     1861 gccgctctcg cgggccttac gcgacttgac gtggttgaca ttattctcaa cgcgatccac
     1921 cgaagtggcc ttatcaaaga gcttgctgaa agtggggctg ccccacttgg tcaggtgtga
     1981 gagctcatgg gagaagtagg tcgtaaaaga gctgttcaac tcagcctggg agaatataac
     2041 atgaccgtcc tggcctactc tggcagcgga actgaacctg ggtgagtcat actccacccg
     2101 gttgaaggcg tcgatgaagg atggcttgtc gctcaaagtt acttctgtga tcttctggct
     2161 ctggggcagg gcttccaaaa gcggtatcac atcttcgggt agcaccctgt ttcggagcgg
     2221 atgcacagct aatgcctttc tcgcttcgaa catggccaca actttggtca cctcgttagg
     2281 cggcaagact tcagcgaagc ccctggcgtc accctgcgca gcctgagctt ccaacttgct
     2341 cttgatcgac tcgggcaagt tacgatagct ctccggcatt tcggcacgca gccgcaaggc
     2401 gcgtacttca ttgagctgga cggcatattc ttgcggcacc gatatgcggg tggaaagctt
     2461 atccacctga taaaccacgt ggtcgcgggg cttgaactca agcaatttat tgaactccgt
     2521 ccaggaggcc tctttggctc caggaggctt gatcagcttc accagatcct cgtgtttggc
     2581 ggaaacatcc gccttcaatt cgaattcatt gcggctctca gtgcccacca ccttcagctt
     2641 aggttgcaat tctttgatag aaacagcttc aaagggttta aaggaaagcc cctcgtatgc
     2701 ctgcatgcgc ccctcatccg accagaggta agcgatgcgc tcttcaaggg tcatgcggcg
     2761 ccgctctctc acgctggttt cggcatcact ggcagaagtg gtgcgagcga gggcgtcggg
     2821 gccgtcagcc ttgagacctt tgcccggggc tgtttccggt acatccactg gcttgccgac
     2881 agcttgttca tggggcgctg atttttgcga aaggggtcgg tgtaccttct cggcaccacc
     2941 catgagggca ccggttccca taccgatcaa gccgccttcc atgacgccgt tcatgatatg
     3001 gcttatgctc atcttctccc ttgccgcttc cacgccgccg aatacggcac caccggcacc
     3061 accgccggct gctcccgtaa ccacattttt caccacggtc tgggtgacgc tgttttcggc
     3121 gctgcctaaa agaacagagg atgcccgccc tactctcaaa ccaagaaatc cggcgggcac
     3181 actgagggtg ccgcccaatc cggtgcccac ggcaaaatta gtgatggtgc tgccggctga
     3241 gaaattacct tccgcgtctt tgtaggtgct gctctgaaag gctgtcttca
//
LOCUS       DAOQNZ010000283         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14518_length_3288_cov_3.391277, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000283 DAOQNZ010000000
VERSION     DAOQNZ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14518_length_3288_cov_3.391277"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKW73_06375"
     CDS             <1..457
                     /locus_tag="PKW73_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72942.1"
                     /translation="ASTPVVQSETPPVSTPVVQSETPPASTPVVQSETSPTSNSAGQT
                     FTSEESKPTFKSETGAQDKAADRAARQEEERARREGERKRRAEEHARHDEEHRQEHLR
                     HDKDHDDDDDDKRDMEREKEREAERERERKKEREKELLRESEKRDDDKD"
     gene            complement(509..1690)
                     /locus_tag="PKW73_06380"
     CDS             complement(509..1690)
                     /locus_tag="PKW73_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72943.1"
                     /translation="MTRESKVEEQATDYSFLAGGLVYRLLNRVGLMKPDLRGAWGRVL
                     VMVLITYVPLLALACVQGVGFGSAVKIPFFHDLSEVCRFLLVPPLLILAEFVVEPWLL
                     QVVKHARENLIDASELEKLDKFLSSSLRWRNSNLVELALLIFTFGSVWFEAQVMPHST
                     VSSWRQLPNTDAPTLAWYWYANFAKPLLRFIWLRWLWRYLLWSFFLFRLSRLSLSIRP
                     THPDQKGGLAFIATGHARFAIIAFAFGVQSTGILADQILWYGRTLFSFKYEIAGALMF
                     MLFIFLTPLLAFTGKLLDGKRIGLFQYGALADEYTAAFHEKWIRCERGNEPLLGTGDI
                     QSLADLGNSYSVVREMQVCLIDKGNVMTFAAATLTPFVPLLLTVYPFDELVKNLLKAI
                     L"
     gene            complement(1747..2961)
                     /locus_tag="PKW73_06385"
     CDS             complement(1747..2961)
                     /locus_tag="PKW73_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNA72944.1"
                     /translation="MTDCSQLSDGARAILPGSVIDKADIRLEVLLDHFLEPLSLDGQR
                     WEFLVSQSEASGFMQSLYWARLKQAQGMRVTQLILRSGGDIIGGALLYTAPRNNGAGI
                     LSAPDGPVLPWADAALAGRGLSLIMEAAESLASKLGVMSLRIEPRLPLPVPKLLREFG
                     RSLSDQNPQETLFVSLAGQVEDILSTMRHKGRYNIAQSAKRGVRVLEEPSGSPESLRR
                     FYGLLSEAGVRNGFLVEPFEFFEDLVEAFSEGDMPSDLPSGLSGSPLTGEGDHSLPAV
                     GKTSSCSPQIRFFFAELDGDTVGALLMVVYGRRATYLYGGVSGRRREAMCGYALQWAA
                     MQSARALGCVEYDMYGYDQFRSPDNEYARFSQFKEQFGGEVRRFIGAQEHYFVEDLAG
                     AVIKAIKQANLN"
     gene            complement(2986..>3288)
                     /locus_tag="PKW73_06390"
     CDS             complement(2986..>3288)
                     /locus_tag="PKW73_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72945.1"
                     /translation="QQELSDYQSSAADSGNQGPGGQGLASQGAGQTAGMSGGDGQGGG
                     DMVQFGSRMIPRKEAIRRTSIAIKRTQKKIDSINSQLSSLPAESLAASKMLSALKV"
BASE COUNT          900 a          922 c          851 g          615 t
ORIGIN      
        1 agcatctacc ccggttgtac aatccgagac accaccagta tctactccgg ttgtacagtc
       61 tgagacacca ccggcatcta ccccggttgt tcagtctgag accagcccca catccaattc
      121 cgccggtcaa accttcacca gcgaggaatc caagccaacc ttcaaaagcg agaccggcgc
      181 gcaagacaaa gccgcagata gagccgccag gcaggaagaa gaaagagccc gccgcgaagg
      241 cgagcgcaag cggcgggcgg aggaacatgc caggcacgat gaagagcaca ggcaggagca
      301 tttgcgccac gacaaagacc acgatgacga tgacgacgat aagcgcgaca tggaacgcga
      361 aaaggaacgt gaagccgagc gcgaaagaga gcgtaagaaa gagcgcgaaa aagaacttct
      421 gagagagtca gaaaagcgcg acgacgataa agactgaaga cttaagattg gaaactgaag
      481 actgcttgcg gtgcagtgcg tctgaaatct agagaatggc cttgagcaga tttttgacaa
      541 gctcgtcaaa gggataaaca gtcaatagaa gcggcacaaa gggagtcagg gtggcagccg
      601 caaaagtcat gacattgccc ttgtcgatga ggcagacctg catttcgcgc accacactgt
      661 aactattgcc caggtcggca agagactgta tatcgccagt accaagcagc ggctcattac
      721 ctcgctcgca tcttatccac ttttcgtgaa aggcggccgt gtactcatcg gccagggctc
      781 cgtattggaa gagccctatg cgtttgccat cgagcaactt accggtgaag gccagaagcg
      841 gcgtcaaaaa gatgaagagc atgaacatga gagcgccggc tatctcatat ttaaatgaga
      901 acaaagtacg cccgtaccat aagatttggt cggcgagaat gcctgttgac tgcacaccga
      961 aggcaaaggc aattatggca aatcgcgcat gcccggtagc aatgaaggcc aacccgccct
     1021 tttgatcagg atgggtgggt cggatgctga gtgaaagtct cgacaatctg aagaggaaga
     1081 aactccagag caaatagcgc cagagccaac gcagccagat gaagcgaagc agcggcttgg
     1141 caaaattggc gtaccagtac caggccagtg tgggggcatc ggtattaggc aattgccgcc
     1201 atgaactgac agtcgaatgt ggcatcacct gcgcctcgaa ccagactgaa ccaaaggtga
     1261 agataagcag tgccagctcg actaaattcg aattacgcca gcgcaaagaa gagcttagaa
     1321 acttatccag cttctccagc tcggaagcat cgatcaaatt ttccctggca tgttttacca
     1381 cttgcaagag ccagggctct accacaaact ctgccagaat gagcagaggc ggcaccaata
     1441 gaaatcggca gacctcgctc agatcgtgaa aaaagggaat cttgacggca gagccaaagc
     1501 ccacaccctg aacacaggcc agtgcaagaa gcggcacata ggtaattaag accattacca
     1561 gcacccggcc ccaggcaccg cgcagatcag gtttcataag cccgacccgg ttcagaaggc
     1621 gatagaccag tcctccagcc aggaaggaat aatcagttgc ttgctcttca actttacttt
     1681 ctctggtcac acaaaggttc cttttcttcc attacatata gacgccaggg tccgagaacc
     1741 aattggtcag ttcaagttgg cctgcttgat ggctttgata acagccccgg ccaggtcttc
     1801 aacaaagtaa tgctcctggg ctccaatgaa gcgcctgacc tcgccgccga actgctcctt
     1861 gaactgtgaa aaacgagcgt attcattatc aggggatcta aactgatcgt agccgtacat
     1921 gtcgtattca acacagccca gtgcccgggc gctctgcatg gcagcccact gcagggcgta
     1981 accgcacatt gcttcccgcc tgcgcccgct cacaccgcca tataagtaag tcgcacgcct
     2041 gccgtacacc accattagta gtgccccgac cgtgtctcca tccaattcgg cgaagaaaaa
     2101 tctgatttga ggggagcatg acgaagtctt gccgacggca ggcaaagaat ggtctccttc
     2161 gccggttaac ggggaacctg acaaaccgga cggcaaatcg gacggcatat caccctccga
     2221 aaatgcttcc accaggtctt caaaaaactc aaagggttca acaagaaatc cgtttcgcac
     2281 tcccgcttcc gaaagcaagc catagaagcg gcgtaagctc tcgggagacc cggacggctc
     2341 ctcaagcacc cgcacacccc gtttggcgga ctgcgcgata ttgtagcggc ccttatgccg
     2401 catagtgctc aagatgtcct caacctgacc ggccaagcta acgaagagcg tctcttgcgg
     2461 attttgatcg ctgagggaac ggccaaactc tctgagcagt ttcggcaccg gcagcggtag
     2521 tcgcggttcg atgcgcaaag acataacacc cagtttagag gcaagcgact ccgccgcctc
     2581 cataataagg ctgagaccac gcccggcaag cgcggcatca gcccagggca aaaccggtcc
     2641 atcgggtgca ctgaggatgc cggcaccatt gtttctaggg gcggtataga gcaaggcacc
     2701 accgataata tcaccgccgc ttcgcaaaat gagttgcgtc actctcatac cctgagcctg
     2761 tttcaagcga gcccaatata gactctgcat aaagccgctt gcttcagact gactgaccag
     2821 gaactcccaa cgctgtccgt caagagagag aggctcaaga aagtggtcta acagcacttc
     2881 cagccggata tcggctttgt cgatgacaga gcccggcaaa atagcacggg caccatcgct
     2941 taattgagag caatcagtca ttacgcctgt tctcactcct taaccttaaa ccttgagagc
     3001 cgacaacatt ttgctcgcag ccagactttc agcaggcagg gaagaaagtt gactgtttat
     3061 agaatcaatt ttcttctggg tgcgcttgat ggcaatggaa gtacggcgaa tcgcttcttt
     3121 gcgcgggatc atgcgactgc cgaattgcac catatcgcca ccaccctgac cgtcaccgcc
     3181 ggacatgccg gcagtttgac cggcaccctg gctagcaaga ccctggccgc ccggaccctg
     3241 attgccacta tcggcggcac tgctttgata gtcggaaagt tcctgctg
//
LOCUS       DAOQNZ010000284         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14640_length_3270_cov_3.515086, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000284 DAOQNZ010000000
VERSION     DAOQNZ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14640_length_3270_cov_3.515086"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKW73_06395"
     CDS             <1..183
                     /locus_tag="PKW73_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72946.1"
                     /translation="ELKEEVAPVLDSRYVSMEGSFMRLSDKPMDADELNYLFPKLPDR
                     VLNIETASGLFRKSLS"
     gene            343..1233
                     /locus_tag="PKW73_06400"
     CDS             343..1233
                     /locus_tag="PKW73_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNA72947.1"
                     /translation="MSESIAEPLFITEETSPFASADAGKEAQQLLEDGRQFADGSVER
                     WLVRLYERNAGGITRVLDGSGAVIGTGFVVGTGLIATDLSVARILGEEPAHLRFISGC
                     RQRARVLAFDSRAEVALLQAAELPADGIVALSLSKPTTLKSDKVACIGFHRANDFQEP
                     VVSSGILRGIKFDKRVELISRISGAPDCCGSPILDRQGRVIGLYNKRKVGLDKGAGAE
                     HLRAMVRAVRLVGVPEGKVLQVVSRVGQEPHTVAIGKRDYPSSLLSEDLQKIRASLEY
                     RRHVENCLTVSVVSAKPVNF"
     gene            1336..2928
                     /locus_tag="PKW73_06405"
     CDS             1336..2928
                     /locus_tag="PKW73_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNA72948.1"
                     /translation="MINQSGKAHPELTILIASMCAFAGVVAFCQFIGRPLPQNRQTMA
                     ESTSRDDPGDAGHVPTSGRYYRNNQLVDNHARPAPADTDQPAADRPPDAALNAEKVSP
                     AAMADQSAQALAGEGSGLAPQPLLPVDLGNQSLTAPAAQGGVKADGESEVSPESAAAV
                     GEPVRLSMAPGGILHPGLKFGPASLPEGAPSASGGTSVSAESSAPGSAQGSAGMVGGK
                     SGHAAQNSPLYPSQAPASSMDGAPAPTNHESDKNSAAALVGPSLVPQGLNTVAGSRFA
                     RLASARGGLPAPTNDALYAVVMRNYGATFVARPGLKLPEQALFDKPAQVRAYQKSLPL
                     KQARMGAYRVTLQKDALEALLAARKDAEQLHLKISPVGDIASLRTYEDTERIWRKYLE
                     KGLGYWVGRKKMTAAEAAAILASPPRKQLNTVLALEQKGYYLHANRMHSLLNLAAPPG
                     GSQHISGLALDVEEHKNPKVRAILNRHGFYQTVLHDTPHFIYLGRSEKELPDLGLTLV
                     VSDGRQFWVPDIPPVETIISAK"
     gene            3063..>3270
                     /locus_tag="PKW73_06410"
     CDS             3063..>3270
                     /locus_tag="PKW73_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72949.1"
                     /translation="MEKIKKKKKKKKKRKRKMSINPFEWYGPPYVVFYAITGCSAAIV
                     GWAVKQKVLGKLAADAVITRNEAES"
BASE COUNT          703 a          850 c          952 g          765 t
ORIGIN      
        1 gaactgaaag aagaagtagc tccggtgctg gacagtcgct atgtttccat ggaaggtagt
       61 tttatgaggc tttccgataa gcccatggat gccgatgaac tcaactatct ctttcccaag
      121 ctgcctgaca gagtgctgaa tattgagacg gcctcgggac tcttccgcaa gtcgctgtca
      181 tagacgttgc ttctgagcat ctttaaccga taggctcgca agaagccctg ccgctgggct
      241 tgtctcactt ttcagtgccc gccgtttgcc acaatttagc gctagactgg ttgttataaa
      301 gcctgattgc aggcggtctt caacggaatc tcgcttgtcc ccatgtctga atcaatcgcg
      361 gaaccactat ttataaccga agagacctcg ccatttgcct ctgccgatgc gggaaaggag
      421 gctcagcagc ttcttgagga tggacggcag tttgccgatg gttcggtgga gcgctggcta
      481 gttcgcctct atgagagaaa tgccggcggc attactcgcg ttctggacgg cagcggtgcg
      541 gtaatcggca ccggctttgt ggtgggcacc ggattgattg ctactgattt gagcgtggcg
      601 cgcatcctgg gtgaagaacc ggcccatctt agatttatca gtggttgccg gcaaagggcc
      661 agggttctgg cctttgatag ccgagctgag gtagcgctgt tacaggcggc tgaactgcct
      721 gccgacggta ttgtcgcact gtcgctctcc aagccgacga cgttgaaatc cgacaaggtg
      781 gcttgcatcg gttttcatcg cgccaatgac ttccaagagc cggtggtctc ttcgggaatt
      841 ttgaggggca tcaaatttga taagagagtg gagctgatca gtcgtatctc cggtgctcct
      901 gactgctgtg gcagcccaat actcgatagg caggggcgtg tcattggtct ttataacaag
      961 cgtaaggtgg ggcttgataa gggtgccggc gccgaacacc tgcgagccat ggtgcgagcg
     1021 gtgcgcctgg tgggagtgcc ggaagggaag gtgcttcagg ttgtttcgcg agtcgggcag
     1081 gagccgcaca cagttgccat tggtaagcgt gactatccct ccagcctgct cagtgaagac
     1141 cttcagaaga tcagggcatc ccttgaatac agacggcatg tggagaactg cctgactgta
     1201 tcagtggttt cggctaaacc ggtcaacttc tgagcgtctg atatccgcct aatattaggt
     1261 tgggtggtgt ttataggacc ttttgctatt tgctcgatag ccttgctgcg gttaaactgt
     1321 ggtgggggtc gcaatttgat aaaccagagc ggtaaggcgc atccggaact gacaatttta
     1381 atagcctcaa tgtgtgcatt tgctggggtg gtggcctttt gtcagtttat cggtagacct
     1441 ttgcctcaaa accgccagac catggctgaa tccacttccc gggatgaccc tggtgatgcc
     1501 ggtcatgtgc ccaccagtgg gcgctactac cgcaacaatc agttggttga caaccatgcc
     1561 cgcccggccc cggctgatac cgatcaacct gctgccgacc ggccgccgga tgccgcgctg
     1621 aatgcggaaa aggtttcgcc tgccgcaatg gcggaccagt cggctcaggc tttggccggt
     1681 gagggaagcg gcctggcgcc acagccctta ctgcctgtgg atctcggcaa tcaaagtttg
     1741 acagcccctg ccgctcaggg cggcgtcaag gctgatgggg agagtgaagt tagcccggaa
     1801 tcggccgctg ctgtcggtga accggtgcgt ttgagcatgg ccccaggcgg tatcttgcat
     1861 ccgggtttga aatttggacc cgcttccctg ccggaaggcg cccccagtgc ttccggagga
     1921 acatcagtta gcgcggaaag ttctgcgcca ggttctgcac agggctctgc cggaatggtc
     1981 ggcggtaagt ccggtcatgc cgctcaaaac tcaccgctct atccttctca ggcacctgcc
     2041 agttccatgg acggagcgcc ggcccccacc aaccatgaaa gcgataagaa cagtgccgct
     2101 gccctcgtgg gtccctccct tgtgcctcag gggctgaaca ccgtagccgg ttcacgtttc
     2161 gcacgcctgg ccagcgctcg cggtggtttg ccggcgccca ccaatgatgc actttatgcc
     2221 gtggtgatgc gcaactacgg tgctactttt gtggccagac cgggtctaaa attgcctgag
     2281 caagccttgt ttgataaacc tgcccaggtg cgggcctatc aaaagagtct gccgctcaag
     2341 caagcgagaa tgggagccta tcgagttact ctacaaaaag atgctctgga ggcgcttttg
     2401 gcggcacgca aagatgccga gcaattgcat ctcaagatta gccctgtagg tgacatagcc
     2461 agcttgcgca catatgagga caccgagagg atctggcgca agtacctgga gaagggtctg
     2521 ggctattggg tcggtcgcaa gaaaatgacc gcggcggaag cggccgccat tctcgccagc
     2581 ccgcctcgca agcagctcaa tacagtcttg gctctggagc agaaaggcta ttatctgcac
     2641 gccaatcgca tgcactctct tctcaatctg gcggcgccgc cgggagggtc tcagcatatt
     2701 tccggactgg ctctggatgt ggaagagcat aaaaatccga aagtaagagc cattctcaat
     2761 cggcatggct tctatcaaac cgttctgcac gacactcccc attttattta tttagggcgc
     2821 agcgagaagg aactgcctga tcttggtttg accctggtgg taagcgacgg cagacaattc
     2881 tgggtgccgg acattccgcc ggtggaaacc atcatctccg ctaaatgagc gccggctctc
     2941 gatctctgcc tggttctcga tacagctttg cgagccggtt ttttcgccgc ctgagataag
     3001 ggttttatct attcttaatt ccggtttaac ttctccttta accagcagaa gctaggctgg
     3061 tagtggaaaa aataaagaag aagaaaaaga aaaagaagaa gagaaaaaga aaaatgagta
     3121 taaatccttt tgaatggtac ggtcctcctt atgtagtgtt ttacgccatc accggttgca
     3181 gtgcggccat cgtgggttgg gcggtgaagc agaaagttct gggaaagctg gctgccgatg
     3241 cagttatcac cagaaatgag gctgagtctc
//
LOCUS       DAOQNZ010000285         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14664_length_3267_cov_3.829390, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000285 DAOQNZ010000000
VERSION     DAOQNZ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14664_length_3267_cov_3.829390"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PKW73_06415"
     CDS             <1..860
                     /locus_tag="PKW73_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA72950.1"
                     /translation="LDLKCPYGTELKVVSVLDFFEPLPAVEEIKEREIAAARALVDET
                     VEKLKNTHKEAVVTGEVLDGYVVDEILHVSQEWGADLIIMGSHGRTGMAEFWMGSVSR
                     SVLLHARSAVRIVRDKSGDDQPSGGNVLLAVDDSEHCDHLIEHVIAMPWTAKTTFRLV
                     HVVPSINPDMLLDSDTGYANTLSQYCDAKVAGAEIWVRELVGKLNAIYGDNVAESRIV
                     IGDAREEILKQANEWPADLVMLGSHGRRGFERLLMGSVSEAVATHAKCSVEVTRMPAR
                     RKPKLHVVK"
     gene            870..>3267
                     /locus_tag="PKW73_06420"
     CDS             870..>3267
                     /locus_tag="PKW73_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72951.1"
                     /translation="MTIHHIRCLSLSLCLSLGLSLPVYEGVFFSPAFAVEPIYSGYFD
                     AGKKFFKEKRYQDALRQFEMALSNSEHLKPGSDGMIALYDLLGQAYCHCGNQRKAEEY
                     LSKCLAMRETSPKKNELEMFGTVMLLGTVYRNENKFAESETLYKRGLSLFQGSGPIKY
                     LCQATVLYALGSLYLERAKGPESQTALEQALKLRDKTILKSALSEQGIYDALGRSYQM
                     QGRMSDAETMLKRALELAVKAKDLDSTAYSEANLAGVYIELSRYDEARKLLESSIKLI
                     EKTHGGDSTHIATLYSNLAEVAINQDRYEEAEDLLNKALAIHQKHLGPRHTSVGYDLS
                     KLISVLRNQGKYQKAEELGRQCSDIFITALGADNQTTAQSYKLMSGVLLDQNRLPEAA
                     RYADDAMKIFENLGLTQEQAEAMVSLAEIKALSNDKSAARTLFETACGLMEKNKDADP
                     SYLARTYSDLAQIHTQAGELAAAEQDMRRALALREKIYGNESARVVSDLRALVKNLNA
                     QKKVDEANQVQVRIDALTKKMPDLTVKATPNLTAPIAEVAGVVAPRLLNKWALVIGIS
                     NFADPDINLRYAAKDAVDFSNYLIQDAHFPAGNVRLLTDKDATRDNIIKHLGPEWLGK
                     RARSDDLVVVYVSSHGSTAKSEAGGVNFLVAHDTQPDALLSSGIPMQWLSQIIKEQVH
                     SRRVVLILDVCHSGSASAGEKGLRRENNFNVGAVPIGEGQAIICSSAPEQVSWESRTY
                     PNSVFTRRLIEALKQNDGKVNLNQAFSYLRDSVESEVLRDRGKLQTPVYFSKSWQGPP
                     P"
BASE COUNT          786 a          837 c          887 g          757 t
ORIGIN      
        1 ctctcgatct gaagtgccct tacggcacgg aactgaaggt ggtttcagtg ctcgatttct
       61 ttgagccgct gccggccgtg gaagaaatca aggagcgtga aattgccgct gctcgcgccc
      121 tcgtggatga aaccgtggag aagctcaaaa atacccataa agaagccgtc gtgaccggcg
      181 aagttctgga cggctatgtg gtggatgaaa tcttgcatgt cagccaggaa tggggcgccg
      241 acttaatcat catgggttcc catggtcgca ccggtatggc tgagttctgg atgggcagcg
      301 tctctcgttc tgtgctcctg catgcccgct cggcagtgcg tattgtgcgc gataagtccg
      361 gtgacgatca gccttccggc ggcaatgtct tgcttgccgt cgatgattcc gagcactgtg
      421 atcacttgat cgaacatgtg attgccatgc cctggaccgc caaaacaacc ttcaggttag
      481 tgcatgtggt gccttccatc aatcccgaca tgcttctcga ttccgatacc ggctatgcca
      541 ataccctttc ccagtattgc gatgccaagg tggcaggggc ggagatttgg gtgcgagagc
      601 tggtgggcaa actcaatgcc atatacggcg ataacgtagc cgagtcgcgc attgtcatag
      661 gtgatgcgcg ggaagaaatt ctcaaacaag ctaatgaatg gcctgccgat ctggttatgc
      721 ttggctcaca tggcagacgc ggctttgagc gtctgctcat gggcagtgtt tcggaggcgg
      781 ttgctaccca tgccaaatgt tcagtggaag taacgcgcat gccggcccgc cgcaagccca
      841 aactgcatgt ggtcaagtaa caggcttcaa tgacgataca tcatatacgg tgcctgtccc
      901 tctcgctttg cctcagtctg gggctttcct tgcccgtcta tgaaggcgtt ttcttcagcc
      961 ctgcttttgc cgttgagcct atttactccg gttatttcga tgccggcaag aagtttttca
     1021 aagaaaaacg ctatcaagat gctttgcggc agtttgagat ggctctcagt aatagtgagc
     1081 atctcaaacc aggtagtgac gggatgattg ccctttatga cttgctcggt caggcctatt
     1141 gtcattgcgg caatcagcgc aaggcggaag agtatctctc aaagtgtctg gccatgagag
     1201 aaaccagccc gaagaaaaat gagctggaaa tgttcggcac ggtcatgttg cttggtaccg
     1261 tctatcgcaa cgaaaacaag tttgccgagt cggagactct atataagcga ggactgtcgc
     1321 tgtttcaggg ctccggtcca atcaaatatc tttgccaggc caccgtactt tatgctcttg
     1381 gttccctcta tttagaaagg gctaagggac cggagtccca gaccgctctt gagcaagcct
     1441 tgaaactgcg cgacaaaacg atactcaagt ctgccttgag cgaacagggc atctacgatg
     1501 cgcttggacg cagctatcaa atgcaggggc gtatgagcga tgccgagaca atgctcaaaa
     1561 gggctctcga actggccgtg aaggcaaaag atctggatag taccgcctac agtgaggcta
     1621 atctggccgg tgtttatatt gagctttccc gctatgatga agccagaaag ttactggagt
     1681 cgtccatcaa gctcatagaa aagactcatg gcggcgacag tactcatatt gccaccctgt
     1741 attccaatct agccgaggtg gccatcaatc aagatcgcta cgaagaggcg gaagatctgc
     1801 tgaataaggc ccttgccatt caccagaaac acctcggacc caggcatact tccgtgggct
     1861 acgatctaag caagcttatc tcagtgctgc gcaatcaagg caagtatcag aaagccgagg
     1921 aactcggccg gcagtgcagt gatattttca tcaccgccct tggtgccgat aatcaaacca
     1981 cggcgcagtc ttacaaactg atgtcgggcg tgctacttga tcaaaatcgc ctgccggaag
     2041 cggccaggta tgccgatgac gccatgaaga tctttgagaa tctcggtctg acacaggagc
     2101 aggccgaagc catggtctct ctggccgaga taaaggctct cagtaacgat aagtctgccg
     2161 ctcgcacgct ttttgaaact gcctgcgggc tgatggaaaa aaataaggac gcagacccct
     2221 cttatctggc ccgcacctac agcgacctgg ctcaaattca tactcaagcc ggagaactgg
     2281 ctgccgccga gcaggatatg cgaagggccc tggccttgag ggagaagatt tacggtaacg
     2341 aatcggctcg tgtggtgagc gatttgcgcg ccctggtgaa gaacttaaac gctcagaaga
     2401 aagttgatga agccaatcag gtgcaagtgc gcatcgatgc tctcaccaag aaaatgcctg
     2461 atttaaccgt caaggctact cctaacttga cggctcccat tgccgaagta gccggtgtgg
     2521 tggcgccccg gttgctcaat aaatgggccc tggtaattgg catcagcaat tttgccgatc
     2581 ctgatataaa tttgcgttat gccgccaagg atgccgttga tttttcaaat tatttgattc
     2641 aagatgccca cttccctgcc ggcaatgtca ggctccttac cgataaggat gccaccaggg
     2701 acaatatcat caagcatctc ggaccggaat ggctggggaa aagagcgcgc agcgatgatc
     2761 ttgttgtagt gtatgtctcc agccatggca gcacggccaa gagcgaagcc ggtggcgtca
     2821 acttcctggt tgcccatgat acgcaaccgg atgcgcttct atcgagcggt attcccatgc
     2881 aatggctctc tcagatcatc aaagagcagg ttcattcgcg ccgggttgta ctgattctgg
     2941 atgtctgcca cagcggctct gcttctgccg gagaaaaagg actgaggcgg gaaaataact
     3001 tcaatgtggg cgccgtgccg attggtgaag ggcaggccat aatttgctcc agcgccccgg
     3061 agcaagtctc ctgggaatca agaacctatc ctaattcggt tttcaccagg cgactgatcg
     3121 aagccctcaa gcagaatgac ggcaaggtta atcttaatca ggcattctcc tatttacgcg
     3181 acagtgtgga gtcggaggta ttgcgtgatc gcggtaaact gcaaacaccg gtatacttca
     3241 gcaaaagctg gcagggaccg ccgcccg
//
LOCUS       DAOQNZ010000286         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14677_length_3265_cov_3.496262, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000286 DAOQNZ010000000
VERSION     DAOQNZ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14677_length_3265_cov_3.496262"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1351)
                     /locus_tag="PKW73_06425"
     CDS             complement(233..1351)
                     /locus_tag="PKW73_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72952.1"
                     /translation="MKSQAVKALLLVSTLLATSIVIEQSAEARGGGGHGGGGGRGGRA
                     MGGRGLRGNLGGGHEGGLQGLRSRFGGGEGQTGGEGLGRFAGQGERSANRSQAGQSWR
                     GRFGQGNGSNSTTGSHSSLSESGLAPAGTRQATVRDYGSAGNNARTSSHEGTTPNGGD
                     YSRDRSVSYSKDTGLTKTVSQQGTTAKGGEYDASKTMTYSKSDGFSKDTSVQGTTAAG
                     GTYDRSKQVTAGQGQGYDKTVNMTGTTAAGGTYDRNKQVTAGQGEGYDKSVNTTGTTS
                     SGGTYDRNKQVTAGQGEGYDKSVNMTGTTSSGGTYDHNKEVSAGQGEGYSKAFNTTGT
                     TASGKNYSRSRGRGRGYRPPEEVSESENSDKANAGASK"
     gene            1593..2663
                     /locus_tag="PKW73_06430"
     CDS             1593..2663
                     /locus_tag="PKW73_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72953.1"
                     /translation="MGIGKFQHSKLMVGFLACTLLLSAGAAGRCENFSGILKSRLARG
                     NAALPAAVSSSKCQTLYDDWRDKERNRTVPVKIYLPLGAASRQVAPMPVVIFSHGLGG
                     SREAALYLGESLAEHGYVAVHLQHPGSDESLWKSSGARDVNAIRGELSKGANYSNLKL
                     RIEDVKFVLDELARRNQSDSRLAGKLDLGRIGLAGHSFGAGTTMALAGQNYGAGRFNM
                     NFADARVKAALYLSPPVNLRGRKPEEVYGKIKIPGMLMTGTEDNSPIGDTTAQERLLP
                     FSGIQAPNQYLVNFKDGDHGIFGGRRRKGKDEDGGFQTMISRLSIAFFDAYLKGDQQQ
                     LQWLKDDAAGYLGGRAVFEKKG"
     gene            2709..3200
                     /locus_tag="PKW73_06435"
     CDS             2709..3200
                     /locus_tag="PKW73_06435"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNA72954.1"
                     /translation="MPPERSASRKAAKSKKALRSSAGAPEKESQPGCEKPVFHQVYEL
                     VQRIPVGRVLTYGLISHKLNKRLSAQGVGWALNALPPAARPQGGKSGAAEAAFHSGNV
                     PWHRVVNARGGLSTHKIADIPPDMQMQLLMAEGVVFQDELLDLERYLWMEGLHVPDSG
                     RNS"
BASE COUNT          622 a          898 c          926 g          819 t
ORIGIN      
        1 cccttcggcg cattggaatc ggaggcaatg gaatgatcga tttcggcatg atgaaatggc
       61 atagaaaatg ggctcctgtc atatctttgg taagcgcaaa agacgaagca ggtgttttgc
      121 ttgtccgttg ctgtggacag gctaatatct gcttcgtcaa attgacgtca aattagtcga
      181 cgctgacggg cggttcagag atggtcagac ctggttgtat cgatgccgtc ccttacttgc
      241 ttgcaccggc attggcctta tcgctgtttt cgctctcgct gacctcttcc ggcggccggt
      301 aaccgcggcc gcgaccgcgg gaacggctgt agtttttgcc gcttgccgtt gtgcccgtgg
      361 tgttgaaggc tttgctataa ccttcgccct ggccggcgct cacttctttg ttgtggtcat
      421 aggtgccgcc actgctggta gtgccggtca tattgacgga cttgtcgtag ccctcgcctt
      481 gtccggctgt gacttgttta ttgcggtcgt aggtgccgcc actgctggta gtgccggtgg
      541 tgttgacgga tttgtcgtag ccctcgcctt gtccggcagt gacttgcttg ttgcggtcgt
      601 aggtgccgcc ggcggcagtg gtgccggtca tgttgacggt tttgtcgtag ccctgaccct
      661 gaccggcggt gacttgcttg ctgcggtcgt aggtgccgcc ggcggcagtg gtgccctgta
      721 cgctcgtgtc tttggagaag ccgtcggact tgctgtaagt catggtttta gaagcatcat
      781 actcaccacc cttggcggtt gtgccctgtt gtgagacagt tttggtgaga ccggtgtctt
      841 tgctgtaaga gactgatctg tcgcggctgt aatcgccgcc gtttggggtg gttccttcat
      901 gactggaagt gcgggcattg ttgcctgccg aaccgtagtc tcttacggta gcctgtcttg
      961 tgcctgccgg tgccagacca ctctctgaaa gggagctatg gctgcctgtg gtgctgttgc
     1021 tgccattgcc ctgaccgaag cgcccgcgcc aactctgacc cgcttgtgaa cggttggcgg
     1081 agcgttcgcc ttgcccggcg aagcggccca gaccctcgcc gcctgtttgc ccttcaccgc
     1141 cgccgaagcg actacgcaga ccctgcaagc ccccctcatg accaccacct agattgcctc
     1201 tcagccctcg gccgcccatg gcccgtcctc ctctgccgcc gccgcccccg tgaccgccgc
     1261 cccctcgagc ttccgcactt tgttctatga caatagaggt agccagtaag gttgatacca
     1321 gaagtaaggc ttttactgct tggcttttca tggaatttct ccgaaactcg ggggtagttt
     1381 atgaggtctt ggacggcgca cgtctctctg gttaatcaaa cgtttcggct tggcaaaaag
     1441 ttcaattcct cgggaacatt taatctgccc atgcactcat ctgccttttt tgaagatgaa
     1501 tttgcagaat taaggagttt ttgttgaact tttgtgggtc gttttccgta ttaaggaaag
     1561 taccgctaca ttttgacaga gggccagcga aaatgggaat cggcaaattt caacatagta
     1621 aattgatggt ggggttcctg gcctgcaccc tgcttttgag cgccggtgcg gcagggcgct
     1681 gtgagaattt ctcgggtatt ctcaagtcgc gtctggctcg cggcaacgcc gccctcccgg
     1741 ctgcggtttc ctcgtcaaag tgccagacct tatatgatga ttggcgcgac aaagaacgca
     1801 atcgcaccgt gcccgtaaaa atatacctgc cccttggcgc cgcctcccgt caggtggcac
     1861 ccatgccggt ggttattttc tcccatggtt taggtggttc ccgcgaggcc gctctttatc
     1921 tgggtgaaag cctggccgag cacggctatg tggccgtcca cctgcaacat ccgggcagcg
     1981 atgaaagtct gtggaagtcc agcggcgccc gcgatgtaaa tgccattcgc ggcgagcttt
     2041 ccaaaggggc caactacagc aatctgaagt tacgcattga agatgtgaag ttcgtactcg
     2101 atgagctggc caggcgcaac cagagtgatt ctcgcctggc cggtaagctt gatctcggtc
     2161 gcattggcct tgccggccac tccttcggtg ccggtaccac catggccctt gccggtcaaa
     2221 actacggggc gggcagattc aatatgaact ttgccgatgc cagagtgaaa gccgctctct
     2281 atctcagtcc ccccgtcaac ttgcgcggtc gcaagccgga agaagtctat ggaaagatca
     2341 aaattcccgg catgctcatg accggcacgg aagataactc gcccataggt gataccaccg
     2401 cccaggaaag gttgcttccc ttcagcggca tccaggctcc caatcaatat ctcgtcaatt
     2461 tcaaagacgg cgaccacggc attttcggtg ggcgcaggcg caagggtaaa gatgaagacg
     2521 gcggctttca aactatgatc tccaggctgt ccattgcttt cttcgatgcc tatttgaaag
     2581 gcgatcagca gcaactgcag tggcttaaag acgatgcggc cggttatctt ggcggcaggg
     2641 ccgtatttga aaagaagggt taaactaggg tgccgcttat ctgaatgtag ttgttgaagg
     2701 gtaccaattt gcctcctgaa cggtccgcct cccgcaaggc tgctaaatcg aaaaaggctt
     2761 tacgctcaag cgccggggct ccagaaaaag agtcacagcc aggttgcgag aagccggtct
     2821 tccaccaggt ttacgagctg gtgcagcgca ttccggtagg gcgcgttctc acctatggat
     2881 tgatttccca taaattgaac aagaggcttt cggctcaggg agttggttgg gctctcaatg
     2941 cattgccacc ggctgcccgc cctcaaggcg gcaaatccgg ggcagccgag gccgcttttc
     3001 attccggcaa tgtaccctgg caccgggttg taaacgccag gggcgggctc agcacccata
     3061 aaattgccga tattccaccg gacatgcaga tgcaactctt gatggccgag ggggtggttt
     3121 tccaagacga actgctcgat ttagagcgct atctctggat ggaagggctt catgttcccg
     3181 attccggtcg aaattcatga ttatttctct cccatgcttt tcatagtctg aaatggcatg
     3241 cttaagctgg cttcctgcgc accag
//
LOCUS       DAOQNZ010000287         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14817_length_3250_cov_3.677934, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000287 DAOQNZ010000000
VERSION     DAOQNZ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14817_length_3250_cov_3.677934"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKW73_06440"
     CDS             <1..288
                     /locus_tag="PKW73_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72955.1"
                     /translation="DRAVFHFLTADQDRKAYVRQALRAVKPGGHVIVATFGPDGPKQC
                     SGLNVVNYDSQSLHDQFGIKFKLVQADTELHGTPFGTTQQFLYCFCKVSTD"
     gene            393..1493
                     /locus_tag="PKW73_06445"
     CDS             393..1493
                     /locus_tag="PKW73_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72956.1"
                     /translation="MSARGTHPTNELTAASAQDSNPESRFNLSKDTQASFNSLELYRG
                     LSDLPKHSDQGSATLPRVDVMDTGNSLKREPIILAQAQERGSSRDFQGDRPSQTKPEG
                     EAPARAWEMNLKHNSDRHGHPDALVYLPRGFDPGKPVNLMIYNHGWNTTAGKSYRDAK
                     LGEQMAGAPPNTVLIVPEWQQSPGAESGRQGNLSKPGMYAGMLQEILDKTPGLQGKKL
                     KDIENIGIISHSAGYNPTEKMLADGTLGPKVKSLTLLDSAYNPAGMEGWLRANAPALA
                     RGEKRFTNIFNDTTGESQMQLKTIKAILGERGLPQEGLLVDQGNAKRPLQPEQLANKS
                     IVFKRSDVTDGKQGPHGSMPGLYVKTIASSLR"
     gene            1576..>3250
                     /locus_tag="PKW73_06450"
     CDS             1576..>3250
                     /locus_tag="PKW73_06450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA72957.1"
                     /translation="MKICPTCLTNFADDISKCPKDDADLILDENQPKSTLGPGHIFAG
                     QYEILSILGKGGMSVVYRARHRLMDRIVAIKVLQGDAEAIAIERFKQEAKSASLLNHP
                     NIISIYDFGIFGEQAYLVMDCLEGKTLADVLETEGHLNADRAVKLFRQACLALENAHK
                     HGVIHRDLKPSNICLVPDENGHEHLKIVDFGIAKLMNKTGLQQLQLTQTGEVFGSPLY
                     MSPEQCTGKSLDNRSDIYSLGCLMYESLTGKPPIVGSTAYETMALHVSGKPEPFSKAA
                     PELKIDPSIEALVFRCLEKRPIDRYQSAAEILSDLPTILPESGSMKIKQVEHPTKQKR
                     EMKRLRYAFWTLFVLLAAVLSYMIVDDGADNDKGTVLEKTIWNAETTMAQTCMDWHWY
                     ELSRAILLHTEHTATTKFSNRARLLTCLNLEYNLYKKARMFEDLERVSDLIALTHEEI
                     IVNMCDRLEKDLDELEAGDSNATSSVNRVIAPYVIDRLRHAVNGLASQSKDQRAEVIL
                     LRAKQIMSNLLGKQDSLIADLNVMLAECYHRQERQNKVRPLLQEALKIYE"
BASE COUNT          868 a          923 c          828 g          631 t
ORIGIN      
        1 gaccgagctg tctttcactt tctgacggca gatcaagatc gaaaggccta tgtcagacag
       61 gccctgcgtg ccgtcaaacc cggcggacac gtgattgtag ccaccttcgg ccctgatggt
      121 ccgaagcaat gttccggctt gaacgtggtc aactatgaca gtcaatccct gcatgatcag
      181 tttgggatca agttcaagct ggtgcaagcc gacacggaat tgcacggcac tcctttcggc
      241 accactcaac aatttctcta ttgcttctgc aaagtcagca cggactgata aggctggcgg
      301 caaggtccgg ggaatttcac gaatttttca ttagctgaga cctactctct agtagctggg
      361 ctaattcaaa tttcgaattc tcggagctta ccatgtccgc cagaggcacc catccaacca
      421 atgaactgac tgccgcttcc gctcaagaca gcaaccctga gtcgcgcttc aacttgagca
      481 aagacactca ggcttccttt aacagtcttg agctctatcg gggcttgagc gacttgccga
      541 agcactcgga ccagggctcc gctactcttc cccgagtaga tgttatggac acaggcaata
      601 gcctgaagag agagccaatc atcctggccc aggcacagga acgcggaagc agcagggatt
      661 ttcagggaga ccgaccctct cagacgaagc cggaaggaga agctccggca agagcctggg
      721 aaatgaactt aaagcataat tccgaccggc acggacaccc cgatgccctg gtctatctac
      781 cccggggctt tgaccccggc aaaccggtca atctaatgat ctacaaccac ggttggaaca
      841 ccacagccgg aaaatcttac agagacgcca aactgggcga acaaatggcc ggtgcgccgc
      901 ccaataccgt cttgatcgtg ccggaatggc aacaatcgcc aggggctgaa agcggcagac
      961 aagggaattt gagcaagccg ggcatgtatg cgggcatgct gcaggaaata ttggacaaga
     1021 caccggggct acagggcaag aaactgaaag acatagagaa catcggcata atctcccatt
     1081 ccgccggtta caatcccacc gagaaaatgc tggccgatgg caccctggga ccgaaagtaa
     1141 aaagcctcac attgctcgat tcagcctaca accccgccgg catggaaggc tggctcaggg
     1201 caaatgcacc agcgctcgcc cgaggcgaga agcgcttcac caacattttt aacgacacca
     1261 ccggggaaag ccagatgcag cttaaaacca tcaaagccat actgggcgaa cgggggctac
     1321 cgcaagaggg attactggtg gaccagggaa atgctaagcg ccctctacag ccggagcaac
     1381 tggctaataa atccatagtt ttcaaacgca gcgatgtgac ggacggcaag cagggccccc
     1441 acggcagcat gcccggtctc tatgtgaaaa caatcgcctc ctcgcttcga taggaaagga
     1501 tgcgtgcgtg gaatatcctg ggcagctata aatagccgtt aactgttaat tattcgatta
     1561 gcgccccggt gaagcttgaa aatctgcccc acctgcctta ctaacttcgc tgacgatatc
     1621 agcaaatgtc ccaaggacga cgctgacctg attctggatg agaaccagcc caaaagtacc
     1681 ctcggccccg gccatatttt cgccggacag tacgagatct tgtcgattct aggcaaaggc
     1741 ggcatgagcg ttgtctaccg ggcccgtcat cgcctcatgg atcgcatcgt ggccatcaag
     1801 gtcttacagg gcgatgcgga agccatcgcc atcgagcgct ttaaacagga agcaaaatcg
     1861 gccagcctgc tcaatcatcc aaatattatc agcatctacg acttcggcat ttttggcgag
     1921 caggcttatc tggtcatgga ctgcctggag ggcaagaccc tcgctgacgt gctagaaacg
     1981 gaaggtcatt tgaacgccga ccgagccgtg aaacttttcc ggcaggcttg cctggccctg
     2041 gaaaacgccc acaaacacgg cgtcatccac cgggacttga agcccagcaa tatttgcctg
     2101 gtacctgacg aaaacggaca tgaacacttg aagatagtcg acttcggcat cgccaagctc
     2161 atgaataaaa ccggcttgca gcaactacag ctaactcaga ccggcgaggt cttcggctca
     2221 cctctttata tgagccctga gcagtgcacc ggcaaaagcc tggacaaccg ctcggacatt
     2281 tattccctgg gctgcctcat gtatgagagc ctgaccggca agccgcccat tgtgggcagc
     2341 accgcctatg aaaccatggc tctgcatgtc tcgggaaagc cagagccctt cagcaaagcc
     2401 gcccctgaac tgaagatcga tcccagcatt gaagccctgg tcttccgctg tctggaaaag
     2461 cgccccattg acagatacca gtctgccgca gaaatacttt ccgatcttcc caccattttg
     2521 cctgaatcag gctcaatgaa aataaagcaa gtggagcacc ccaccaagca aaagcgagag
     2581 atgaagcgac ttcgctatgc cttctggacg cttttcgtgc tgcttgctgc ggtgctctct
     2641 tacatgattg tcgatgacgg cgccgacaac gacaagggca ccgtgctgga aaaaaccatc
     2701 tggaacgccg aaaccactat ggcccagacc tgtatggatt ggcactggta cgaactgtcc
     2761 cgggccatcc tgctgcacac ggagcacacg gccacaacca agttcagcaa cagggcaaga
     2821 ctgctcacct gtctcaatct tgaatacaat ctctacaaaa aagcacgtat gttcgaagac
     2881 ctggagcggg ttagtgatct cattgccctc acccatgaag agatcatagt aaatatgtgc
     2941 gacagattgg aaaaggatct ggacgaactg gaagccggcg acagtaatgc caccagtagt
     3001 gtcaaccgtg tgattgcccc ctatgtaatc gaccggctca gacatgccgt gaatggactg
     3061 gccagccagt ctaaggacca gcgagcagaa gtaatattgc tgcgcgccaa gcaaatcatg
     3121 agcaacctac tgggcaagca agactcactg atagccgact tgaatgtcat gctggccgaa
     3181 tgctaccacc ggcaggaacg gcagaataag gtgcgccccc tattgcaaga agcgctaaaa
     3241 atctacgagg
//
LOCUS       DAOQNZ010000288         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14835_length_3248_cov_3.208268, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000288 DAOQNZ010000000
VERSION     DAOQNZ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14835_length_3248_cov_3.208268"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PKW73_06455"
     CDS             complement(<1..810)
                     /locus_tag="PKW73_06455"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNA72958.1"
                     /translation="MNVADRIDFDVETEASSSCGPPSHWGFAFKLPAWLGFLDSDHFP
                     SGRSEVLNLPVKSRASRWFPFIFLHFFALLALFHKPSPFAICLAVFLYFFRMFAITAF
                     FHRYFSHRTFKTSRAAQFLFAFWGGLAVQRGALWWASHHRNHHRYSDRDGDIHSPIKS
                     GFIWSHIGWITADCNMPTRYEIIGDFSRYPELVFLNRFDWLPPLFLGLALYGLGCFLE
                     LHYPSLSTSGNELVLWGFFVSTVVLFHGVCSINSLSHIWGSRPYRTDDSSKN"
     gene            complement(888..2126)
                     /locus_tag="PKW73_06460"
     CDS             complement(888..2126)
                     /locus_tag="PKW73_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HNA72959.1"
                     /translation="MSGSDEKYLVGAQAAEFLGVKPATLYAYASRGQIESVPGATGRE
                     RCYRLSDLIRLRQSARGVKSAKDSEPAVWTGPAIKSAITEIREDGHRYRGQSAIELAL
                     QRTSFEDTAELLWDTGGSAALWAGLEPFECNSFKGLVSPDTECLELLKLLVVGLEMSD
                     PVARKLNRDDVYDTARRLILTMAMSIGLPAGRSQYVIDDQLDVNNFVGCILARALSES
                     PCREQSEMINLALVLCADHELNASALASRIAASCDASLYSCLLSALGSFSGSLHGSAS
                     RRAEDIVTASLGFSSARAWLKDYLRQNDTIAGFGTELYSKGDPRARVLIDCALSASAA
                     DERLIRLKEIVECVAEELSLFPNLDVGLAAVTYALNLPQGAGSAIFAVSRSAGWIAHA
                     IEQRLYGGVIRPRARYIGKC"
     gene            2308..>3248
                     /locus_tag="PKW73_06465"
     CDS             2308..>3248
                     /locus_tag="PKW73_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72960.1"
                     /translation="MNNLVQRIEVALPHVETILNPAAQDFIRALEQRFRPELDELLKK
                     RQDRQKQIDQGIFPDFLPHTEHIKSGTWQVAPVPADLEDRRVEITGPCERKMMINALN
                     SGARVFMADLEDSLTPTWANIISAQMNLIEAVRGTLSFTSAEGKEYKLQMDRRLATLV
                     VRPRGLHLLERHLLIDEKPVSASLFDFGLYFFHNAHELIKRGSAPYFYLPKLESHEEA
                     RLWNDVFNFSQDYLQIERGTIRATVLIETILAAFEMEEILYQLKEHAAGLNAGRWDYI
                     FSLIKKTRNHDFTLPDRAKVTMVVPFMRAYAQLLVAT"
BASE COUNT          883 a          876 c          853 g          636 t
ORIGIN      
        1 attcttgctg gaatcatcag tgcgataagg tcggctgccc catatgtgag atagagaatt
       61 gatggagcag acgccgtgga agagcaccac ggtggagacg aagaagcccc acaagacaag
      121 ctcattgccg ctggtggaaa gggaagggta gtgtaattca aggaaacaac ccagtccgta
      181 aagagccaag cccagaaaga gaggcggcag ccaatcgaag cgattgagaa agaccagttc
      241 cggataacgg ctgaagtcgc caataatttc atagcgcgtg ggcatgttgc agtcggcagt
      301 gatccagccg atatgggacc atatgaaacc gcttttaatc ggtgaatgaa tatcgccgtc
      361 gcgatcggaa tagcggtgat ggttgcgatg atggctggcc caccacaaag cgccgcgctg
      421 caccgccaac ccaccccaga aggcgaagag aaactgagcg gcgcggcttg tcttaaaagt
      481 gcggtgggaa aagtagcgat gaaagaatgc ggttatggcg aacatgcgga agaaataaag
      541 aaaaaccgcc agacagatag caaagggact gggcttatga aaaagagcca gaagggcaaa
      601 gaagtgcagg aaaatgaagg gaaaccaacg gctggcccgc gactttacag gaaggttcaa
      661 cacctcactg cggccggacg gaaagtggtc actatctaag aaacccagcc aggccggcag
      721 tttgaatgcg aagccccagt gagagggagg cccgcaagaa gaggaggctt cggtctctac
      781 atcaaaatca atacggtcgg cgacgttcaa gtcgatgcct cattcacgaa gaactcctac
      841 tgatagcaga ttagcatggt ctatgtgaac gcaaactgaa cgagtctcta acacttaccg
      901 atgtatcgag cccttggacg aatgacgccg ccataaagtc tctgttctat ggcatgggca
      961 atccaaccgg cggatcggct cacggcaaag atagcagaac cggcgccctg aggcaggttc
     1021 agcgcatagg tcacagcagc aagaccgaca tcaaggttag ggaaaagcga aagctcctca
     1081 gccacacatt ccactatttc cttcaagcgt ataagccttt cgtcagcagc ggaagccgag
     1141 agggcgcagt ctatcaggac tctcgcccgg ggatcgcctt tgctgtaaag ctcggtaccg
     1201 aagcctgcaa tagtgtcatt ttgccgcaag taatccttca accatgccct tgccgaggaa
     1261 aaaccaaggg aagcagtgac tatgtcttcc gcccgccgac tggcagagcc gtgcaagcta
     1321 ccactaaagg aacccagcgc gctcaacaga caactgtaga gggaggcatc acaagaggcc
     1381 gcaatgcggg aggcaagtgc cgatgcattc aattcgtgat cggcacaaag caccagggct
     1441 aaattgatca tttcggactg ttcgcggcaa ggagattcgg acagagcccg cgccaggata
     1501 cagcccacaa aattgttcac atccagttga tcatctataa cgtactggct gcgaccagcc
     1561 ggcagaccaa tactcatagc catggtcaga atcaaacgcc tggccgtgtc gtaaacatca
     1621 tcgcggttga gtttgcgggc taccggatcg gacatctcaa gtcccaccac cagaagcttg
     1681 agcagttcaa gacactccgt atccggagag accagtccct taaaagaatt gcattcgaag
     1741 ggctcaagcc cggcccagag ggcagcgctg ccaccggtat cccagagaag ttcggccgta
     1801 tcctcaaaac tggtcctctg caaggccagc tcgatggcgc tctggcctct gtaacgatgc
     1861 ccgtcctctc tgatttcagt tatggccgat ttaatggccg gaccggtcca gacggccggt
     1921 tcggagtctt tggccgactt tacacctctg gccgactgcc ggaggcgaat caagtcactg
     1981 agccgataac aacgttcccg gccggtggcc ccgggcacgc tttctatttg cccgcggctg
     2041 gcataggcat agagagtggc cggcttaaca ccgagaaact ccgccgcttg ggcaccgaca
     2101 aggtattttt catcagatcc agacatggca gctatgatac cagccacctg tcccgaggaa
     2161 ttgcagggcg cttctatata gatggaaatt ctccgaatta tgcaaagatt aaattagtga
     2221 tttattgatt gcaatcgatc aatatatcaa tataattacc ataacaaagc tctacataaa
     2281 ccgtcaaaag tcccggagga tttctggatg aacaatctag tacaacggat tgaggtggcc
     2341 ctgccgcacg tggaaaccat tttgaatccg gccgcccagg acttcatccg ggctctggaa
     2401 caacgattcc gccccgagct tgacgaactt ctcaagaaac gccaggacag acagaagcaa
     2461 attgatcagg gcatcttccc cgacttcctg ccccacaccg agcacattaa aagcggcacc
     2521 tggcaggtgg ctccggttcc cgccgacctt gaggacaggc gcgtagagat taccggaccc
     2581 tgtgagcgaa aaatgatgat caatgccttg aactcgggcg cccgagtttt catggccgat
     2641 ctggaagact ccctcactcc cacctgggct aacataattt ccgcccagat gaatctgatc
     2701 gaggcagtga gaggtaccct cagcttcaca tctgcggaag gcaaggaata caaactgcag
     2761 atggaccgga ggcttgccac tctggtggta agaccgcgcg gtctgcacct gctggagagg
     2821 cacctgctca tagacgagaa accggtttca gccagtcttt tcgacttcgg actctacttc
     2881 ttccataatg cccatgaatt gatcaaacgg ggcagcgctc cctacttcta cctgcccaaa
     2941 ctggaaagtc atgaggaagc caggctctgg aacgatgttt tcaatttttc ccaggactat
     3001 ctacagattg aaaggggcac catccgcgct accgtcctaa tcgagacgat tctagcagcc
     3061 tttgaaatgg aagagattct ctaccagtta aaagagcacg ccgccggtct gaacgccggt
     3121 cgctgggact atatcttcag cctcatcaaa aaaaccagaa accatgattt caccttaccg
     3181 gatcgcgcta aagtgaccat ggtcgttccc tttatgagag cctatgccca actcctggtg
     3241 gccacctg
//
LOCUS       DAOQNZ010000289         3247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14846_length_3247_cov_4.071429, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000289 DAOQNZ010000000
VERSION     DAOQNZ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14846_length_3247_cov_4.071429"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PKW73_06470"
     CDS             complement(<1..1004)
                     /locus_tag="PKW73_06470"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNA72961.1"
                     /translation="MNGKDVSIFWFRQDLRLEDNPALTAALASEHCYLVYIWAPEEES
                     PWEPGGASRVFLHESLSDLERQIAAAGGRLHFFKAGRGQTFSSSLSALQAIAENCGAR
                     SIYWNRRYEPEVIRRDSEIKAALKASGLTVETFNGALLKEPWQVKTKEGKPFQVFTPF
                     WRACLNELDLEPPLPVPADLHRAGNLGQALPDTVSVPDLALLPSISWHKSIVASYAAG
                     EREAHQALEHFLKNALANYSIDRDKPHRQGVSRLSTYLHFGQISPRTVWHRTKAYSAR
                     SKNCDEAAAAFLRELGWREFSHHLLYHFPETTQAPLRKDFLNFPWQKDEQLLKAWQKG
                     L"
     gene            1279..1827
                     /locus_tag="PKW73_06475"
     CDS             1279..1827
                     /locus_tag="PKW73_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72962.1"
                     /translation="MAEKTIRVGELLTRAGILRKQDLDEACNIAQDTGQMIGKVLIMS
                     GFVTKDDLQAAVEAQSLVRDGHLELELSFMAVSAASRGRITLEQALDQLGWQPQTSMP
                     SARLGELLLSSGVISPEQLNQALNEVRESITPLGAVLIAMGAITENVLRDALAVQSEI
                     RQGKRTKPDGVALLSKFARAQA"
     gene            complement(1862..2920)
                     /locus_tag="PKW73_06480"
     CDS             complement(1862..2920)
                     /locus_tag="PKW73_06480"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNA72963.1"
                     /translation="MASPKTGHVNGNITAERQFLPCLSLVLSLCLSLSPTALAKGSKQ
                     PKGPVYGGAGPQAQADMPAGLIPVVIPADGVMREFFVHIPPILQESINAGQPPPSLPM
                     VMVFHGGLGLASRMDTMAGFDPIADREGFITVYPQAINRHWHDGRNIEGQDKYDDVAF
                     VNAIIDYMQRRYRIDHQRVYACGISNGGFFCQYLALHMPDKLAAIASVAATLPSIVAS
                     ARPALKPMSVLFILGMNDPLMPFKGGPLAFANFKDRGMVVSAADAAQFWVKANRGNKV
                     PTSVDLPDTDPSDNCRVKVANFSGGLKGSEVLVYGIEGGGHTWPGAAEVLPKNLVGET
                     CRDISASEVIWDFFKRHM"
     gene            complement(2941..>3247)
                     /locus_tag="PKW73_06485"
     CDS             complement(2941..>3247)
                     /locus_tag="PKW73_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72964.1"
                     /translation="PTGEHRQITSAKSPSRRYNENRWRQIKFIVQMTVGLGLCFLIMN
                     NSSTMWETTVKIGKSFGCDLENNALFVGFQNSMVNWGKQKAEAIKQQKKGSKGKNSH"
BASE COUNT          657 a          834 c          974 g          782 t
ORIGIN      
        1 agacccttct gccaggcttt cagaagttgc tcgtctttct gccaggggaa gttgaggaag
       61 tctttgcgaa gtggggcctg ggtggtttcc ggaaagtggt agagcaggtg gtgggaaaac
      121 tcgcgccagc ccagttctct aagaaaggcg gcggctgctt cgtcgcagtt cttgctgcgg
      181 gcgctgtagg ccttggtccg gtgccagaca gtcctgggac tgatctggcc gaaatgcaga
      241 taagttgaga gccttgatac gccctgccgg tgcggcttat cgcggtcgat actgtaattg
      301 gccagggcat tcttgagaaa gtgttccagg gcctggtgtg cctctctttc ccctgccgca
      361 taactggcga cgatgctctt gtgccagctg atcgaaggca gaagcgccag gtccggcact
      421 gataccgtgt ccggtagtgc ttgaccgagg ttgccggcgc gatgcaggtc ggccggcacc
      481 ggcaggggcg gctctagatc cagttcattc aggcaagccc gccaaaaggg ggtgaaaacc
      541 tggaagggtt tgccctcctt tgtcttgacc tgccagggtt ctttcaggag ggcgccgttg
      601 aaggtttcca ccgtcagccc tgaggctttc agggcagcct tgatttcact gtcgcgcctg
      661 attacctccg gttcgtagcg gcgattccag taaatgctgc gggcaccaca gttttcggcg
      721 atggcttgca gcgcagaaag gctggaggag aaggtctgac ctcgaccggc cttgaagaag
      781 tgcaggcgcc ctcctgcggc ggcaatctgc cgctccaggt ctgagaggct ctcatgtagg
      841 aagacccgac tggcaccgcc cggctcccag gggctctctt cctcaggggc ccagatatag
      901 accagatagc agtgctcgct ggccagagcg gcggtcaggg ccgggttgtc ctccagtctc
      961 aggtcttgtc tgaaccagaa tatgcttacg tctttaccgt tcatatattt ctccacaccc
     1021 ttaaactttc ctctttatct gggaactaag cgtgaacgcc aatttgaata tgaactgaga
     1081 tagggaagaa atgttgccgg gtgcagggtc tcaggctgcc gtgccggtgc attttggaaa
     1141 cattacttta ttatcggttt aacatatgcc cggtccgaga attatgttta tgcggggtat
     1201 tcttaggggg gcgtctggcg ctgaggtggc aaagttgcca gggggcatgt tcaggcaggc
     1261 gcggcagagg gcagcaagat ggcggaaaag acaatccggg ttggtgaatt gctaaccaga
     1321 gccggcattc tgcgaaagca ggatctcgac gaagcgtgca acattgctca agacaccggt
     1381 cagatgatcg gtaaagtgct tatcatgtca ggctttgtca ccaaggatga cttgcaggcc
     1441 gcggtggaag ctcagtctct cgtgcgcgac ggacatttgg agctggagtt gagtttcatg
     1501 gccgtatcgg cagcatctcg cgggcgcatc accctggaac aggccctgga tcagctgggt
     1561 tggcaaccgc agaccagtat gccgtctgcc aggctcggtg agcttttgct gtcgagcggc
     1621 gttatttccc cggagcaatt gaatcaggct ctcaatgagg tgcgcgaatc tattacgccc
     1681 cttggagctg tgcttattgc catgggggcc attaccgaga atgtactgcg cgatgccctt
     1741 gccgtgcagt ctgaaattcg tcagggcaag cgaaccaaac ctgacggcgt ggcgcttctc
     1801 tccaagtttg ccagagccca ggcctgagct tggtttgcta cactccaggc tttcagccat
     1861 tttacatgtg gcgtttgaag aaatcccaaa ttacttctga ggcgctgata tctcggcagg
     1921 tttcccctac gagatttttc ggcaagacct ccgctgctcc cggccaggta tgaccgccgc
     1981 cctcaatgcc gtagacaagc acttccgaac ctttcagacc gcctgaaaaa ttagctactt
     2041 tcacccggca attatccgaa gggtcggtat cgggaagatc cacggaagta ggcaccttat
     2101 tgccgcgatt ggccttcacc cagaactgcg ccgcgtcggc tgccgagacc accatgcccc
     2161 gatctttgaa gttggcaaag gcaagcggac cacctttgaa aggcatgaga ggatcattca
     2221 tacccaggat gaagagaacg ctcattggtt tcagtgccgg cctggcgctg gccacgatgg
     2281 atggtaaggt ggcggctact gaggctattg cagctaattt gtcgggcatg tgcagggcca
     2341 gatactggca gaagaagcca ccattggaga taccacaagc gtagactcgc tggtggtcga
     2401 tgcggtagcg cctctgcatg taatcgatga tggcattgac aaaggcaaca tcatcgtact
     2461 tgtcttgtcc ttcgatattg cggccgtcat gccagtgtct gttgatggcc tggggataaa
     2521 cggtgatgaa gccctcccgg tcggctatgg ggtcgaaacc ggccatggtg tccatgcgtg
     2581 aagcgagacc taagccgcca tgaaaaacca tgaccatggg caggctgggg ggcggctgtc
     2641 cggcgtttat ggattcctgt aagatgggtg gaatgtgtac gaaaaactcc cgcatgacgc
     2701 cgtcggccgg gatcaccacc ggtatgagtc cggctggcat gtctgcctgg gcctgtggac
     2761 cggcgccgcc atagacaggg cctttcggct gtttgctgcc tttggcaagg gctgtgggag
     2821 acaggctcaa gcagagactc aagacgagac tgaggcaggg gaggaactgt ctttcggcag
     2881 ttatatttcc gtttacgtgt ccggtttttg gggatgccaa cttgagtgct tccttctggt
     2941 ttagtggcta tttttgccct tgctaccttt cttttgttgc tttatagctt ctgctttttg
     3001 tttgccccag ttgaccatgg aattttgaaa accaacaaag agggcgttgt tttccagatc
     3061 acagccaaag ctctttccta ttttgaccgt tgtttcccac atggtgctgc tgttattcat
     3121 gatcagaaag cagagaccca gacccacggt catttgcact ataaatttga tttggcgcca
     3181 gcggttttca ttgtagcgtc tggagggaga cttggctgaa gtaatctggc ggtgctcgcc
     3241 ggtgggg
//
LOCUS       DAOQNZ010000290         3245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14859_length_3245_cov_3.054859, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000290 DAOQNZ010000000
VERSION     DAOQNZ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14859_length_3245_cov_3.054859"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKW73_06490"
     CDS             <1..304
                     /locus_tag="PKW73_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNA72965.1"
                     /translation="DGGATKNEFLVQFQADVLKVPVLRASNVESTAWGVAALAGMTAG
                     LIKNEAELESLWQKDLKLSPAEDKAEQYGGWQAALKAAFACAEAQTPCKEKACSKS"
     gene            complement(309..851)
                     /locus_tag="PKW73_06495"
     CDS             complement(309..851)
                     /locus_tag="PKW73_06495"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HNA72966.1"
                     /translation="MNLSGKIRIDAYDPRWPSFYEVERAGIAASLAPWLAAIEHIGST
                     SVPGLAAKPIIDIMPGLRPDAGKDELDSCVQPMIDLGFTYIGQYESELPERRFFRRTA
                     TDGGAGADAGRQAVNVHLVAVGSGFWQRHLKFRDILRGDAQVCRRYEKLKLELAAVHS
                     VVNDYAEAKTDFIREVLERY"
     gene            1069..1353
                     /locus_tag="PKW73_06500"
     CDS             1069..1353
                     /locus_tag="PKW73_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72967.1"
                     /translation="MQAHKCQETIHLNLEKNKAIVLLGLIASFNNDGALRLKHPAEKK
                     VLWDLECILHGMLSDVSGQAFENKVQQAREITWPTSHSRITHNLTVPSTK"
     gene            complement(1453..2739)
                     /locus_tag="PKW73_06505"
     CDS             complement(1453..2739)
                     /locus_tag="PKW73_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA72968.1"
                     /translation="MHNDNIRQAVKAIALTMVSLTACQSVWAGNTMPPAAPSRLKRPV
                     NSAGDSVLSGSVTQTTLSQPRLSRPDSGLAPAVNQPVGRVGSTYDASTAGNTTDGAQG
                     SLFSSGDKSAFAGAQAPAASSAPAPSSNYPSSFKITEADKSSELFSRGLKAVTELGRA
                     MSQSPQLQEKAKSILKGLASGSGSTAGKAAAGTALKAGTAAGASSAALSAVRIPIVGK
                     VLNKQELNLLANYEIAIVIDKSGSMNETDCPQGLSRWEWCRQQLLSFTSQISPVFKSG
                     ITVALFSSNAQVLRNVSFSLVPDIFSKNVPNGGTYMAKPLSEILDEYFERRDRNPASV
                     RKLLIEVITDGEPSDKGELISVISRASRKMTRADEVRIDFLQIGNEEDGIEALRKLDT
                     KLVGESGAQFDIVTVEPFSAVVAEGLPRALLDTASH"
     gene            2900..>3245
                     /locus_tag="PKW73_06510"
     CDS             2900..>3245
                     /locus_tag="PKW73_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72969.1"
                     /translation="MMSVGSLTDKGVSALELAFDLQGPAAILKACKSVKFVLAGSALL
                     LSWMPLLAAPKAFAQNADLQPALSEQSGSLPILRGPKTGLEKQVERRAAKATRAKVDD
                     SPRPGKVRHSGFS"
BASE COUNT          801 a          884 c          885 g          675 t
ORIGIN      
        1 agacggcgga gccaccaaga acgaatttct agtacagttc caagccgacg tgctgaaagt
       61 gcccgtgctg agagcctcca atgtagagtc tacagcctgg ggcgtagcgg ctctggctgg
      121 aatgaccgcc ggtctcatca aaaatgaggc tgaactggaa agcctctggc agaaagacct
      181 gaaactcagc cctgctgaag ataaagctga gcaatatggc ggctggcaag ccgctctcaa
      241 ggcagctttt gcttgcgccg aagcacaaac accctgcaaa gaaaaagcct gcagcaaaag
      301 ctgagacatc aatagcgctc cagcacctct cttatgaagt cagttttggc ttccgcatag
      361 tcgttcacga cgctgtgaac agccgccagc tcaagcttta gcttttcgta gcggcggcag
      421 acctgagcat cgcctctgag aatatcgcga aacttgagat ggcgctgcca gaaaccacta
      481 cccaccgcca ccaggtgaac attgacggcc tgtctgccgg catcggcacc agccccacca
      541 tcagtggcag tacggcggaa gaagcgacgc tcaggcagtt cgctttcgta ctggccaatg
      601 taagtaaaac ccagatcgat cattggttgg acacaactat ccagttcgtc tttgccggca
      661 tcgggacgga gaccgggcat tatatcgatt attggtttgg cggctaatcc gggcacggaa
      721 gtactgccaa tatgctcgat ggccgcaagc cagggagcca gagaggcggc aatcccggcc
      781 cgctccacct catagaagga aggccagcga ggatcgtaag catcgatgcg aatcttgccg
      841 ctcaaattca taaagccgcc tccctaaaaa ctggcaaggt gccgtcctca ctttcagtgg
      901 gcaaattagc gccccgggag gccagggctc caattttagc tctatttgca atttaatctt
      961 tggtctcccc ctatttttct ccggtccccc gttttcacct ctgtcaggac agctcacccc
     1021 cgagcaacta atcggcaatt caaaacaatc aacaaacgag ggttacaaat gcaagctcat
     1081 aagtgccagg aaaccatcca cctgaacctt gagaagaaca aagcgatcgt actcttagga
     1141 ttaatagcca gtttcaacaa tgacggagca ttaaggctca agcacccggc cgagaaaaag
     1201 gtgctctggg atctggaatg catcctgcac ggcatgctct ctgatgtgag cgggcaggcc
     1261 ttcgaaaaca aagtgcagca agccagggaa ataacctggc ccaccagcca tagccgcatc
     1321 acccataatc tgaccgtgcc cagcaccaag tagaagtttt ttcaacgccg acctgataca
     1381 gaaagacctc cctttagggg aggtctttct gcgtgtcttc gtcaagcggg gggaaaatca
     1441 atttaaacga ggctaatggc tggcagtatc aagcagggcc cgaggcagac cttccgctac
     1501 tacggcggaa aagggctcga cggtaactat gtcgaactgc gcaccacttt cgcccacgag
     1561 ctttgtatca agtttgcgca gcgcttcgat gccatcttct tcgtttccta tctggagaaa
     1621 gtcgattcga acttcatcgg ctctcgtcat tttgcgggag gcgcgactga tcacagagat
     1681 caactcgcct ttgtcgctgg gctcaccatc ggtgatcact tcgatgagta attttcgaac
     1741 actggcgggg ttgcgatcgc ggcgctcaaa atactcatct agaatttcag acagcggctt
     1801 ggccatataa gttcccccat tgggaacatt tttagaaaaa atatccggca ccagggaaaa
     1861 gctcacattc ctgagaacct gggcgtttga tgagaagaga gcgacagtta tgccgctctt
     1921 gaatacaggc gaaatctgac tggtgaaact gagcagttgc tggcgacacc actcccagcg
     1981 gctcaagccc tgcggacaat cggtctcgtt catggaaccg gacttgtcaa ttactatggc
     2041 gatttcatag ttggcaagta gattcaattc ttgcttattc aaaaccttgc ccactatggg
     2101 tatgcgcacc gcgctcaaag cggcagagga agcgccggca gcagtgccgg ccttcaaagc
     2161 tgtgccggcg gcggccttgc cggcagtgct gccgctaccg ctggccaatc ccttcaatat
     2221 agatttggcc ttctcttgca actggggaga ctgactcatg gcccgcccca actcagtgac
     2281 ggctttaagc ccccttgaga agagttcgga gcttttatcg gcttcggtaa tcttgaaact
     2341 ggaggggtaa ttggaagagg gcgccggagc cgaactggcg gcaggggcct gggctccggc
     2401 aaaggcactc ttatcgccgc tggaaaagag ggaaccctgg gcgccgtcag tggtattacc
     2461 agcggtactg gcatcataag tcgagccgac tctcccgacc ggctggttga cggccggcgc
     2521 caaaccgctg tcaggtcgac tgaggcgggg ctggctgagg gtggtctggg tgaccgaacc
     2581 tgaaagaacg ctgtcgccgg cggaattgac cgggcgcttg agacggctgg gtgcggcagg
     2641 cggcatggta ttaccagccc agaccgactg acaagcagta agggaaacca tggtcagggc
     2701 aattgccttg acagcctgcc tgatattgtc gttgtgcatt ttgccagacc tgtttggaag
     2761 ataaagagct tgacggaagt cggtaaagaa agcataccga agacatcgag ggaagagtac
     2821 cgctttacac cggcagatag acgatggtaa gccgcgatgg ttcctgaaga gcctttggca
     2881 gcggagcgga actaaaacta tgatgtcggt tggaagttta accgataaag gggtttccgc
     2941 tttagaatta gcttttgatc tgcaaggacc ggccgccatt ttgaaagcct gcaaaagtgt
     3001 caaatttgtc ctggccggaa gcgccctgct cctgagctgg atgccgctct tggcagcacc
     3061 caaggctttc gcccagaatg ccgacctgca accggcgctc tcagagcagt caggttcgct
     3121 gccgatttta cgtggaccaa agaccggcct ggaaaagcaa gtagagagaa gggccgcaaa
     3181 agctacccgc gcaaaagtcg atgacagccc tcgccccggc aaggttcggc attccggctt
     3241 ctccc
//
LOCUS       DAOQNZ010000291         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14875_length_3244_cov_2.731891, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000291 DAOQNZ010000000
VERSION     DAOQNZ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14875_length_3244_cov_2.731891"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKW73_06515"
     CDS             <1..245
                     /locus_tag="PKW73_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72970.1"
                     /translation="MTVSPDGSLVSLKPKAGGSIDVYPWVKLVAVEFPDGHKQTFYAS
                     DREVGLLKKNPVGFYVNWLPADKQGRHFEWGTLELG"
     gene            326..1153
                     /locus_tag="PKW73_06520"
     CDS             326..1153
                     /locus_tag="PKW73_06520"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNA72971.1"
                     /translation="MHTEPGPSSVVCRRKLFSTWLSVVCLALSGSSAQAQTAADTGAS
                     SQSTQLPSIKLETSSSSSSPSTTQNGNTDHAFQMWTPIFIDVPLSRSRKVRGYFEASP
                     RLGDNLKGMNQLLLRSALGYNFNPHLSGYVGHVWVGNFQPQFLKENRVYQQLGFGHML
                     GKRLQVLHRTRLEERFIQDTGGDCSVRARYLLRLALPLKKRFYAVVSDELFVNLNTVG
                     NGPVSGIDQNRIYAGIGRQVNNNLRLEVGYQQQYVNRTDPTDDKANHILVTSVFVNL"
     gene            complement(1229..>3244)
                     /locus_tag="PKW73_06525"
     CDS             complement(1229..>3244)
                     /locus_tag="PKW73_06525"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNA72972.1"
                     /translation="FRNMLYGTILGDVKRNLQNVSSVVLDECHYMNDSERGTVWEESI
                     IYAPKNIQLVALSATIANARDLTTWIDETHGPTELVVSDYRPIPLRFHYFGERRLYPL
                     LAPGGATNSFLKSRFGKKKPVIRGDKRRRPIGGLYTHPADVLASLSGRNMLPAIYFLF
                     SRRGCEEAMKMARGIPLLLPEEQKELRLRVEEFTRDNPNLASHPHIPYLLEGMSVHHA
                     GMLPSWKGMVEKLFQKGLLKVVFATETLAAGINMPARSTVISAIHKRSDEGHRELMAS
                     EFLQMSGRAGRRGMDEVGHVVVLHNPIESVEDAGKLATAKADPLSSRFTPSYGMVLNL
                     LERHTIDDARDLIERSFGQFLINHQLEPLFTQRLSWEAELPRLQAPLCPAEPGDLNLY
                     AKRLEAIRAKHKQLKQMEKGLKTTAVKARQDAVAALADMKIGIQDLVKEAYAMPCHGC
                     PVQKPCSKQAERVEQLERRIRDLDKRVKRETTYYWRTFEALANILRSEGYIFGNRPTD
                     LGRTAAGIRGQNELFLTSMAMSGLFETLEPTELAAVITALVGDDSRANENMRIRVSPQ
                     VDQALLEVQKTMRRILRLQKEHDIDMPITIGTHLCGLTQMWAEGADWDAIRNATPYDE
                     GDVVRGIRRTLDLARQYVRAPGMPDRVADLCQRVELLLNRDEVSEDF"
BASE COUNT          744 a          914 c          891 g          695 t
ORIGIN      
        1 ccatgaccgt ttccccggac ggtagcctgg taagcctcaa gcccaaagcc ggtggttcca
       61 tcgacgtata tccctgggtg aagttggtgg cggtggaatt tcccgacggt cataagcaaa
      121 ccttctacgc cagcgaccga gaagtcggcc tgctcaagaa aaatccggtc ggtttctacg
      181 tcaactggct gccggcagat aaacagggtc gccattttga gtggggtaca cttgagcttg
      241 gctaaaagcc cgttcagcgt ggaactctct cgaacaattg aatcagccgt taatctctct
      301 ctggaagcag gaaacgcgcc cttcagtgca cactgaacca gggccgtcat ccgtggtctg
      361 ccgccgcaaa ctgttttcga cctggctctc tgtggtttgc ctggccctct caggctcctc
      421 cgcccaggct caaacagccg ccgacaccgg cgcatccagc cagagcaccc agcttcccag
      481 catcaaactg gaaaccagct catcttccag ctctcccagc accacccaaa acggcaacac
      541 cgatcatgcc tttcaaatgt ggacgcccat attcatagac gtgcctctct cccgcagccg
      601 caaggtgcgc ggttactttg aggccagtcc gcgcctgggc gacaacttaa aaggcatgaa
      661 ccaacttctt ttgcgctcag cccttggtta taacttcaat ccccacctga gcggctatgt
      721 cggtcatgtc tgggtgggca attttcagcc gcaattcctc aaggaaaatc gcgtctacca
      781 gcaactgggc ttcgggcata tgctgggcaa acgcttgcag gttctgcacc gcacccgact
      841 ggaagaacgt tttattcaag acaccggcgg cgactgctcg gtgagagctc gctatctttt
      901 gagactggct ctgcctctca aaaagcggtt ctatgccgtg gtctccgatg agctattcgt
      961 caatttgaac accgtaggaa acggccccgt ttcaggtatc gatcaaaacc gaatttacgc
     1021 cggcataggc agacaagtaa ataacaattt gcgcctggaa gtgggctacc agcaacagta
     1081 cgtcaacaga accgacccca ccgacgataa ggccaaccat attctagtta ccagcgtttt
     1141 tgtcaatctc taaagaaagc agccaggcac gcgccgagtt agtgcaccaa agagggcggc
     1201 agcactagag aagcaaaaga cggcaaaact agaaatcttc cgagacttca tcgcggttca
     1261 agagcaactc cacgcgctgg cagagatcgg caacccggtc gggcataccg ggtgcgcgca
     1321 catactgtct ggccagatcg agagtgcgcc tgattccacg caccacatcg ccttcatcgt
     1381 agggcgtggc attgcgaata gcatcccaat cggcgccctc ggcccacatc tgggtgagcc
     1441 cgcatagatg ggtgccgatg gtgatcggca tgtcgatgtc gtgctctttt tgcaagcgca
     1501 aaatgcgacg catggtcttc tgcacttcca gaagggcttg atcaacctgg gggctgaccc
     1561 tgatgcgcat attctcatta gcccgggaat cgtcaccaac gagggcggtg atcaccgccg
     1621 ctagttcggt aggctcaagg gtctcaaaaa gaccgctcat ggccatagag gtaaggaaga
     1681 gttcgttctg ccccctgata ccggcggcgg tgcgacccaa gtcggtgggg cggttgccga
     1741 agatatagcc ttccgacctt aagatgttgg ccagcgcttc aaaagtgcgc cagtagtaag
     1801 tagtttctcg cttaacgcgc ttatcgagat cgcgaatacg cctctccaac tgctccaccc
     1861 gttcagcctg cttgctgcag ggcttctgca ctgggcagcc gtggcagggc atggcatagg
     1921 cttctttgac cagatcttga atgcctattt tcatatcggc cagggctgct acggcatcct
     1981 ggcgagcttt gacggcggtg gtcttgagac ccttttccat ctgcttgagc tgcttatgtt
     2041 tggctcgaat agcctccagc cgcttggcat aaagattcag atcgccgggc tcggccgggc
     2101 aaaggggcgc ctgcaaacgg ggcagctccg cttcccagga aagtcgctgg gtgaagagag
     2161 gctccagttg atggttgata agaaattgtc cgaagctgcg ttcaatcaaa tcgcgagcat
     2221 catctatggt gtggcgctcc agcaagttga gcaccatgcc gtaagaagga gtgaagcggc
     2281 tggagagcgg gtcggctttg gcggtggcca acttaccggc gtcctccaca gactcaatgg
     2341 gattatggag taccaccaca tgaccgactt cgtccatgcc gcgacgaccg gcccgaccgg
     2401 acatctgcaa aaattcggaa gccatcagtt cccggtgccc ttcgtcggag cgcttgtgaa
     2461 tggcgctaat gaccgtggaa cgagccggca tattgatacc ggccgccagg gtctcggtgg
     2521 cgaaaaccac tttcaaaagg cctttctgaa agagcttttc gaccatgcct ttccaactgg
     2581 gcagcatgcc ggcatggtgc accgacatac cttcaagcaa gtacggaata tggggatggg
     2641 aagccagatt gggattgtcc cgggtaaatt cttccaccct cagacgcaat tccttttgtt
     2701 cttccggcaa gagaagcggt atgccgcgag ccattttcat ggcttcttcg cagccgcgac
     2761 gagagaagag gaagtaaatg gcaggcaaca tattgcgccc cgacaaactg gccagcacgt
     2821 cggcgggatg ggtgtacaag ccgcctatgg ggcggcgtct tttgtcgcct ctgataacgg
     2881 gcttcttctt gccgaagcgc gatttgagga aggaattggt agcaccgccg ggagccagaa
     2941 gcgggtaaag ccgacgctca ccgaagtagt gaaagcgcaa gggaatcggc ctgtaatcgg
     3001 aaaccacaag ttcggtaggg ccgtgggttt catctatcca ggtggtgagg tcgcgggcat
     3061 tggcaatggt agccgaaagc gccacaagct gaatattctt gggcgcatag ataattgatt
     3121 cttcccatac ggtaccgcgc tcgctgtcat tcatatagtg acattcgtcg agcaccacac
     3181 tggaaacatt ctgcaagttg cgcttcacgt caccaaggat ggtgccataa agcatgttgc
     3241 gaaa
//
LOCUS       DAOQNZ010000292         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14883_length_3243_cov_3.048306, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000292 DAOQNZ010000000
VERSION     DAOQNZ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14883_length_3243_cov_3.048306"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKW73_06530"
     CDS             complement(<1..702)
                     /locus_tag="PKW73_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNA72973.1"
                     /translation="MSQIEKKERGQVLIIGSGAGGTTTAITLAEAGFDVVVLEEGARH
                     PLTSYGQPPTKAMNQLYRNRGMMPIMGSVPIGYVEGRCLGGSTEINSGFWHRLPPEFL
                     LRWQARFGLQDFSLEALDGHFAWAEDKLRVQDRNGDWPKSTQVFQRGIEAMQWSAQEV
                     RRAASGCVNTNACAAGCPKGAKQGMSVKLVPEAESLGARFITGARAKLIIKHGNKVTG
                     VLADITKPDGSSDLVR"
     gene            complement(703..2340)
                     /locus_tag="PKW73_06535"
     CDS             complement(703..2340)
                     /locus_tag="PKW73_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNA72974.1"
                     /translation="MNSTLKIIKSRGAIVFAGLAISVFLLVMTGRTIKWEVVTNAFKS
                     AVWLPWVPLAVFTYMVGMLLRGLRLQQLVGNEANLTVATASNIIAVGYAVNNILPARL
                     GEFARAGMLAERTGLPYALSLTVTFLERLLDGLTILSLFVIASFITPTQDWMRNASQI
                     AALAFAMVIPCVMMLALFPQMSLSLISQLSHPFGRKLHHKALAITTQVTRGFSCLRDA
                     GSAMVVLFLSFLIWGVEGLMFALVMPCFLMLFSPVKALAVMSFTNLGILVPSTPGYLA
                     VYHRCCSEALQAVTSQQGAGMLTPFLPADVLRVIMPVSVNMVDPSTAISYAVVVHLIF
                     YATVTIWGVIAMARYGMELGTTAALAWEAKPLPLQELEAQAHNFALITSTPVVKNEVL
                     CFKKTPFWRSLTEAMVLPAEEHQLSLAEEERARALEDATDFLCREISSLPPKLAGMLK
                     IGTIGFRILAALISFNTFENLSLKRRSAMVNAWSYGKVSLTRKLFKPLRSLALLAFFE
                     NEAVQAALEKATGDCANPPLKATEEGAIAVEKSEATT"
     gene            2494..>3243
                     /locus_tag="PKW73_06540"
     CDS             2494..>3243
                     /locus_tag="PKW73_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72975.1"
                     /translation="MDNSSDQVRAIDESSLRQYFRRVLEVSPGSGRKQIRQSRDRISG
                     RLRERLLLAQGEERERLERAMVLVNTAFDCLSNNERFTDYFNRVNSGEIDVFTPQEMP
                     PELGPPIKPPPRKQAERRQAHTVERMERIASLIADAVRSAGAEEAHRQTKTDGKLPNA
                     DVFYETIYDVARNAGRRVKKEELAEAEKSGKYNLDDECINELDAVIEDTAEIVAHKEY
                     DRLEYMAAQLPPPQAKNKVTALILAGLILFLV"
BASE COUNT          702 a          857 c          966 g          718 t
ORIGIN      
        1 gcgcaccagg tcggaggagc cgtccggttt ggttatgtcg gccagtactc cggttacttt
       61 gttgccgtgt ttgattatga gcttggcgcg ggcgccggtt atgaacctgg cccccaggct
      121 ttccgcttcc ggaacaagtt taacgctcat accttgtttg gcgcccttcg ggcagccggc
      181 ggcgcaggca ttggtattga cgcagccgga ggctgccctg cgcacttcct gggccgacca
      241 ttgcatggct tcgatgcccc gttggaagac ctgggtagac ttgggccagt caccgtttct
      301 gtcctgcacc cgtagcttgt cttcggccca ggcgaaatgt ccgtccaggg cttccaggct
      361 gaaatcttgc aagccaaagc gcgcctgcca gcgcagcaga aactccggtg gcagccggtg
      421 ccagaagccg ctgttgatct cggtgctgcc tcccaggcag cgaccctcca cgtaaccgat
      481 gggcaccgag cccatgatgg gcatcatgcc tcggttgcgg tagagctggt tcatagcttt
      541 ggtagggggt tgtccgtagc tcgtgagggg atggcgggct ccttcttcca gtacgactac
      601 atcgaaaccg gcttccgcca gggtgatggc ggtagtggta ccgccggcgc cactgccaat
      661 aatgagaacc tgtccccgct ctttcttttc aatctggctc atttaggtgg ttgcctcgct
      721 tttttccacc gctattgcac cttcctcggt agctttcagt ggcgggttcg cacagtcgcc
      781 tgtcgctttt tcaagggctg cctgtaccgc ttcattttcg aagaaggcca gaagagcgag
      841 gctgcgcagg ggcttgaaga gtttgcgggt cagtgagacc ttgccgtaag accaggcgtt
      901 gaccatggcc gagcgccttt tgaggctgag attttcaaaa gtattgaagc ttatcaatgc
      961 cgctaaaatt ctaaagccga tggtgccaat cttgagcata cctgccagtt ttggcggcag
     1021 acttgaaatc tcccggcaga gaaaatctgt ggcatcttca agagcccggg ctctctcctc
     1081 ttcagcgagg gacagttgat gctcttccgc cggcaagacc atagcctccg tcaggctcct
     1141 ccagaaggga gttttcttga agcagagaac ctcatttttc accaccggtg tagaggtgat
     1201 gagagcgaag ttatgggctt gtgcttccag ttcttgcagt ggcagtggtt tcgcttccca
     1261 ggccagagcg gcggttgttc ccaactccat gccgtaacgg gccatggcga tgacacccca
     1321 gatggtgacg gtggcgtaga agatgaggtg caccactacc gcataggaga tggctgtaga
     1381 tgggtcgacc atgttgaccg agaccggcat gattactctc agtacatctg ccggtagaaa
     1441 gggcgtgagc atgccggcgc cctgctggct tgtcactgcc tgtagtgctt ccgagcaaca
     1501 acggtggtag acggccagat agcccggcgt ggacggcacc aggatgccca gattggtgaa
     1561 agacatgacg gcgagcgcct ttacggggct gaacagcatc aggaagcagg gcataacaag
     1621 ggcaaacatg agcccttcta caccccagat gagaaaactg aggaagagca ctaccatggc
     1681 cgagccggcg tcacgcaggc agctgaagcc tcttgtgacc tgggtggtga tagccagcgc
     1741 cttgtggtgc agtttgcgcc caaatggatg gcttaactgg gagatgagcg agagggacat
     1801 ttgcgggaac agggccagca tcattacaca gggaatgacc atggcgaagg ccagggcggc
     1861 aatctgtgag gcattgcgca tccagtcctg ggtgggtgtg atgaagctgg caatgacgaa
     1921 gagtgacaag atggtaagcc cgtccagcag tctttctaga aaggtgaccg tgagagagag
     1981 ggcgtagggc agaccggttc gctcggccag cataccggcg cgggcgaatt ctcccaggcg
     2041 tgcgggcaaa atattgttga cggcgtagcc caccgcaata atattactgg cggtggccac
     2101 ggttagattg gcttcgttgc ccacgagttg ctgcaatctc agacctctga gcagcattcc
     2161 aaccatgtaa gtgaagacgg ccaggggcac ccagggcaac cagacggcgc ttttgaaggc
     2221 attggttact acttcccatt tgatggtgcg cccggtcatg acaagcagga aaacgcttat
     2281 ggcaagtccg gcaaatacaa tggcgccgcg acttttaata atcttgaggg tggagttcat
     2341 tcagcttcct gtcaagtcag gtaagatctt ccctggcaac cactatttaa ctctcttgaa
     2401 aagcaatatt ggaactattt cttattactt accgcccctg cttcataccc tactgtgtgg
     2461 tgagtgccga gatcatgagg aagaaagcta ctaatggata attcttcgga ccaggtccgc
     2521 gccatagacg agtcgtctct caggcaatat ttccggcggg tccttgaggt cagtcccggg
     2581 agcggacgca agcaaatccg ccagtctcgc gatcgtattt ccggtcggtt gcgagagcgt
     2641 cttttgcttg cccaggggga ggagcgggaa cgcctggagc gggccatggt tctggtcaac
     2701 acggctttcg attgtctctc caataatgag cgctttaccg attatttcaa cagagtcaat
     2761 tccggcgaaa tagatgtttt tactccccag gaaatgccgc cggaattggg gccgcccatc
     2821 aagccgccgc cccgtaagca ggcagagcgc agacaggctc atactgtgga gcgcatggaa
     2881 cgcatcgctt cgctaattgc cgatgccgtg cgcagcgccg gtgccgaaga agcccatcgg
     2941 cagaccaaga ccgacggcaa actgcctaac gccgatgtct tttatgaaac catttacgat
     3001 gtggctagaa acgccgggcg tcgcgtcaag aaagaagagc tggcggaagc cgagaagtct
     3061 ggcaaatata accttgatga tgagtgcatc aatgaactgg atgcagtgat tgaggatacg
     3121 gcggagatag tcgcccataa ggaatacgat cgactggagt acatggcggc gcaattgccg
     3181 ccgccccagg ctaagaacaa agtgacggcc ttgattttgg ccggtttgat tcttttcctg
     3241 gtg
//
LOCUS       DAOQNZ010000293         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14895_length_3241_cov_4.111739, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000293 DAOQNZ010000000
VERSION     DAOQNZ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14895_length_3241_cov_4.111739"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /gene="rpsD"
                     /locus_tag="PKW73_06545"
     CDS             <1..362
                     /gene="rpsD"
                     /locus_tag="PKW73_06545"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNA72976.1"
                     /translation="TGLKLLQLLEARLDNLVHRSGIVPSRAQARQLIRHGHFTVNGKV
                     VSIPSYQVRAGEVIAVCEKSKKLVKGILENSPAAAHPNWMDVSIENLTASLVNAPSRE
                     DLDQSIKEALIVEYYSR"
     gene            437..1384
                     /locus_tag="PKW73_06550"
     CDS             437..1384
                     /locus_tag="PKW73_06550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNA72977.1"
                     /translation="MEPLRIKCLENKTGTDGSIYGKFVIEPLEKGYGTTLGNALRRVL
                     LSSLDGSAVSAVRIEGVTHEFTTVPGVVEDVIDVMLNLKGLVVKTFSNDPQYLHLDID
                     RQGPVTGRDIMCPADATIINPDWQLCTLAEGGRLRAEITVERGRGYVPADSHSHFKQA
                     IDVLPIDAVFMPIRKVAYNVEPTRVGESTDFDKLTIELWSNGSIDATEAISQAARQIV
                     EHLLPIAELSGKPMVPAATQPQQQDGKHSSISIEELELSVRAYNCLKRANINSLGELL
                     KLTYDDLMNIKNFGKKSADEVIERLRQFGLTLADTPKDK"
     gene            1422..1847
                     /gene="rplQ"
                     /locus_tag="PKW73_06555"
     CDS             1422..1847
                     /gene="rplQ"
                     /locus_tag="PKW73_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNA72978.1"
                     /translation="MRHRVPGNQLGRPADQRKAVLRALATELLRRDEIVTTLAKAKAV
                     RSCAERLITKGKKGQFGDYKALHEAAKKGDAEAAKKVARVVHLRRQVGAFVYDKEVVT
                     RVFDELAPRYKDRQGGYTRIIKAGPRRGDCTEMAIIQLV"
     gene            1840..2652
                     /gene="truA"
                     /locus_tag="PKW73_06560"
     CDS             1840..2652
                     /gene="truA"
                     /locus_tag="PKW73_06560"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNA72979.1"
                     /translation="MSSSEALSKEKNRIALLIEYTGKKFHGSQYQEGVLTVQQELERA
                     LTILWGEPVRAIFSGRTDTGVSARGQVVHVDLPAFRPPLSEPEIFDLLWRVNGIVIKE
                     LSVVSMVNAPENFHARYSACEREYVYRILNRSQRSSLLKDTHYFVRQPLDLKAMSCAA
                     QALLGEHDFGSFKSSNSDTSASICRVRRAELLNLREGQLEFWIAADHFVYNMVRIIVG
                     TLIDIGLGKRGVESVSEALAKQDRALTGPTAPPWGLTLNAVRYPDHFRLFQL"
     gene            2698..3147
                     /gene="rplM"
                     /locus_tag="PKW73_06565"
     CDS             2698..3147
                     /gene="rplM"
                     /locus_tag="PKW73_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNA72980.1"
                     /translation="MKTYCPKPEEVTREWLVVDAEGKTLGRLAVEVANLLRGKHKPEF
                     TPHVDCGDFVVVLNAEKIQVSGKKETQKLYRHHSGFPGGFKEENLAHLRARRPVAIIE
                     KAVRGMLPHSRLGDQQFTKLKVYVGGKHPHAAQKPAEHELPKHALAN"
BASE COUNT          807 a          875 c          830 g          729 t
ORIGIN      
        1 ctaccggcct caagctcctg cagcttttgg aagctcgtct ggacaatctg gtgcaccgct
       61 ccggtattgt tccctccaga gctcaagcca gacagcttat ccgccacggt catttcaccg
      121 tcaacggtaa ggtcgtatct attccctcct accaggttcg cgccggcgaa gtgatcgccg
      181 tttgtgagaa gtccaagaaa cttgtcaaag gtatcctgga aaactcccca gccgccgctc
      241 atcccaactg gatggacgtc agcatcgaaa acctgaccgc atctctggtt aacgcgccat
      301 cccgcgaaga cctggatcaa agcatcaaag aagcgctaat cgtcgaatac tactcacgct
      361 aatccttagt tagttagata gttagctgcg gatattcgca ttcgcttcac ggaacacatt
      421 cattcacgga gagaatatgg aacctctaag aatcaagtgc ctagagaaca aaaccggaac
      481 cgatggttca atttacggaa aatttgtcat tgagccacta gaaaagggat atggaacaac
      541 cctgggcaat gccctcagac gcgttcttct ttcctctctt gatgggtcgg ctgtaagcgc
      601 cgttcgcatc gaaggcgtca cccacgagtt caccacagta cctggtgttg tagaagatgt
      661 catcgacgtc atgctcaacc tcaaaggtct tgtggttaaa actttcagca acgatccgca
      721 gtacttgcac ctggacatcg atcgccaggg accggtcacc ggccgcgata tcatgtgccc
      781 ggctgatgcc accatcatca accccgactg gcaactctgc accctcgccg agggtggtcg
      841 tctgagagct gaaattactg tagagcgcgg tcgcggttat gtaccggccg attcgcacag
      901 tcattttaaa caagccatcg acgtgctccc catcgacgcc gttttcatgc ccatccgcaa
      961 ggtggcatac aacgtcgaac ctacccgcgt aggagaatct accgacttcg ataagctcac
     1021 catcgagctc tggtccaatg gttctatcga cgccacggaa gctatttctc aagctgctcg
     1081 tcagattgtg gagcacttgc tccccatcgc cgagctttcc ggtaagccca tggtgccggc
     1141 tgccacccag cctcagcagc aagacggcaa acatagctcc atctccatcg aagagctgga
     1201 actttccgtc agagcttata actgcttgaa gagagccaat atcaactccc tgggtgaact
     1261 cctcaagctc acctacgacg atttgatgaa tatcaaaaac ttcggtaaga agtcagccga
     1321 cgaagtgatc gagcgcctca ggcagtttgg tctcacgctt gctgatacac cgaaggataa
     1381 gtagaacagt aagaccataa ggtaagcaaa aggatagagt tatgcgtcac agagtacccg
     1441 gcaatcaatt gggacgtccc gccgatcagc gtaaggccgt tctcagagca ctggccaccg
     1501 agctgcttcg tcgcgacgag attgtcacca ctctcgccaa ggcaaaagct gtgcgttctt
     1561 gcgccgagcg tttgattacg aaaggcaaga aaggtcagtt cggcgattac aaagcgctcc
     1621 atgaggccgc caaaaagggc gatgctgaag ccgccaagaa agtggctcgt gtcgtacatt
     1681 tgcgtcgcca ggttggtgcc tttgtctatg acaaagaagt agtcaccaga gtctttgacg
     1741 aactcgctcc ccgctacaaa gatcgccagg gcggatacac ccgcattatc aaggctggtc
     1801 cccgtcgcgg cgattgcacc gagatggcta tcattcagct tgtctagttc ggaagcgctg
     1861 agcaaagaaa aaaatcgaat cgcccttttg atcgagtata ccgggaagaa atttcacggt
     1921 tctcagtatc aagagggcgt tctgactgtg cagcaggagc tggagcgtgc cctcaccata
     1981 ctctggggtg agccggtgcg ggccattttc agtggtcgca ccgacactgg tgtcagtgcc
     2041 cgggggcagg tggtgcatgt agatctgccg gctttcaggc cgcctttgag cgagcctgag
     2101 atttttgatt tgctatggcg agtcaacggt atcgtcatca aagaattgtc tgttgtcagc
     2161 atggttaatg cccctgagaa tttccacgct cgctattcag cctgcgaaag ggagtatgtg
     2221 tatcggattt tgaacagatc ccagagatct tcgctcttaa aagatactca ctatttcgtg
     2281 cgccaaccgc tcgacctgaa agccatgtcc tgtgcggctc aagcgctttt aggcgaacac
     2341 gacttcggct ctttcaagtc ttccaattcc gataccagtg cttcaatctg ccgtgtaagg
     2401 cgggctgaat tgttgaattt acgtgaaggg cagcttgaat tctggattgc ggccgatcac
     2461 ttcgtgtaca atatggtccg cattattgtc gggactctta tcgatatagg tctcggcaag
     2521 aggggcgtgg aaagcgtgag cgaggcactg gccaagcaag atagagcgct aaccggcccc
     2581 actgctcccc cctgggggtt gacgctaaat gcggtcaggt accctgatca tttccggctc
     2641 tttcagcttt gactttgagc tttgaaatga agggctgacc atcgaggagt aaagagagtg
     2701 aaaacctatt gcccgaaacc agaggaagtc acaagagagt ggctcgtcgt agatgccgaa
     2761 ggaaagaccc tcggacgcct ggccgttgaa gtggcgaatc ttttgagagg caaacataag
     2821 cctgagttca cacctcacgt tgactgcggc gactttgtcg ttgttctcaa tgctgagaag
     2881 attcaagtct ccggcaagaa agaaacccag aagctttata gacaccattc cggtttcccg
     2941 ggtggtttca aagaagagaa tctggcgcac ttgagagcaa gacgccccgt tgccatcatc
     3001 gagaaagctg tgcgcggcat gttgccccat agccgtcttg gtgaccagca gttcaccaaa
     3061 ctgaaagttt atgtgggcgg caaacacccc cacgcggctc aaaagcctgc cgagcatgag
     3121 cttcccaagc atgctctcgc caactagtaa tcagcaatta tcaggaaaaa gaggcaaatg
     3181 tctagcgtaa ttcagtacat cggcaccggc agaagaaagt ccgccaccgc cagagtgcgt
     3241 c
//
LOCUS       DAOQNZ010000294         3240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14904_length_3240_cov_3.918053, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000294 DAOQNZ010000000
VERSION     DAOQNZ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14904_length_3240_cov_3.918053"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKW73_06570"
     CDS             <1..978
                     /locus_tag="PKW73_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72981.1"
                     /translation="RGNNLNFHWDAKVLSVSRRAEKFAVRYDRYRKPGEEKEEVFDYC
                     IVSVGRGGAHWLTQQEFYKALDIYPGQVDIGIRVETSCYFTEEVDKRFYEPKLYYKSR
                     HSGDVVRNFCSNPKGFVTPENHREYVLVNGHSKMYEKSENNNFAVLVSKTFTRPFKDP
                     QMYSQTIAKIVNMLAGGGPLVQRLGDLKAGRRTKSLDNNLIKPTLEAECGDISLAYPH
                     RILQGIVEYLEALDKICPGVAGEHTLLYAPECKSYPDSISVNKNMETNIPNLFIIGDS
                     SSWTRGVGQAATSGILAGEGIKHKLKINVPASDKTTVTATAKEAVSGRK"
     gene            complement(1005..2465)
                     /locus_tag="PKW73_06575"
     CDS             complement(1005..2465)
                     /locus_tag="PKW73_06575"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72982.1"
                     /translation="MDAAKESISWETYLTAGVKAYDLSRLEDAEKLFQKALIEARREL
                     ARLRGDEMDGVVVAVSEELESSSPQVASGKKHSGGLAQASAEDDEQSAKPGLARRVKA
                     PSDNSPEQMRVEDGLAQIFAGMAELYRVMGNLKEAESFAKQALALCESMYGADSMRAA
                     QCLTKLGAVYRAQGRYSSVEPLLRRALTIYENEDLDHGEPLELAQMLETLADFLRAHG
                     KYAQAEPLFQRALAKREACLGAEHPSIGNNLYNLAMLYHAEGKYEQAEPLYERAVRLF
                     EEEWGPEHPSLATVLNYQAELYRSLGDFPRAEKIHWRALKIRQSVLGLEHPSTAQTLS
                     NLALLFHVQCKYDQAEPLYKTLLEIRQKDWGPKHMHVAEVLNSLAEVYHDQGKFSEAE
                     PLYWRAVEIIQENMGHEHPTVAKALRRLAQLFEEQENFDEADRLYRWGLDVSEKSLGA
                     EHPEVAELVSRYAALQRKIIAEDDDDGETTEILWRD"
     gene            2720..>3240
                     /locus_tag="PKW73_06580"
     CDS             2720..>3240
                     /locus_tag="PKW73_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72983.1"
                     /translation="MTALTKKWKLWPLTFDKKAFTPRRIMIDLVKLYLFYVFLSMLII
                     MGLKDRLILFPMQDTSWKPGLKGMDEALKCTESKEVSFKTADGKTLSGYLVKNKNAAA
                     KLVIINHGNAGNIGHRMGIAATLAAAGASALAYDYQGYGESQGDPKLANLIPDATAAY
                     DFATKELGYKADD"
BASE COUNT          799 a         1001 c          779 g          661 t
ORIGIN      
        1 agaggcaaca atctcaactt ccactgggac gcgaaagtgc tctcggtctc gcgccgcgcc
       61 gaaaaatttg ccgtgcgcta tgaccgctac cgcaaacccg gcgaagagaa ggaagaagtc
      121 ttcgactact gcatagtttc cgtcggcaga ggcggcgccc actggctcac ccagcaggaa
      181 ttctacaagg ccctggatat ctatcccggt caggtggaca tcggtattcg cgtcgagacc
      241 agctgctact tcactgaaga agtagataag cgcttctacg agcccaaact ctattacaag
      301 agcaggcatt cgggcgacgt tgtacgtaac ttctgctcca accccaaagg tttcgtcacc
      361 ccggagaacc accgcgaata cgtgctcgtg aacgggcact ccaaaatgta cgaaaagtct
      421 gagaacaaca acttcgccgt gctggtctcc aagaccttta cgcgcccctt caaagatccg
      481 cagatgtact cccagaccat cgccaagata gtcaacatgc ttgccggcgg cggtccgctc
      541 gtgcaaaggc tgggtgatct gaaagcggga cggcgcacca agtcgctcga taacaaccta
      601 atcaaaccca ctctggaagc ggagtgcggc gacatatcac tggcctaccc gcaccgcata
      661 ttgcagggca ttgtggaata tctggaagcc ctggataaaa tctgcccggg tgtggccggc
      721 gaacacacct tgctctatgc tcccgaatgc aagagctatc cggattccat atcggtgaac
      781 aagaacatgg agaccaatat ccccaacctg ttcatcatag gcgactccag ctcctggacc
      841 aggggcgtcg gtcaggcagc cacaagcggc attctggccg gagaaggaat aaagcacaag
      901 ctcaaaataa atgtgccggc ttcagacaag accacggtca cagccacggc caaagaagca
      961 gtgagcggaa gaaaatagtt cgccgctccg accgggagaa attctcagtc tcgccagaga
     1021 atctctgtag tctcaccgtc gtcatcatct tcggcgatta tcttgcgctg caaggccgcg
     1081 tagcgcgaaa ccaactctgc cacctccgga tgctccgccc ctagagactt ttcgctcaca
     1141 tccagacccc agcggtaaag cctgtcggct tcgtcgaaat tctcctgttc ctcgaacaat
     1201 tgcgccaggc gccgcagagc tttggccacg gtgggatgct cgtgacccat gttctcctga
     1261 ataatctcca ccgcccgcca gtagagaggc tccgcctctg aaaacttgcc ctggtcatga
     1321 tagacctcgg ccagtgaatt gagaacttca gccacatgca tatgcttggg accccagtcc
     1381 ttctgcctta tctccagcaa agtcttgtag agcggctcgg cctggtcgta cttgcattgc
     1441 acatgaaaga gcagcgccag attactcaaa gtctgagccg tgctgggatg ctccagcccc
     1501 aggacggact gccttatctt caaagcccgc cagtgaattt tttcagcccg gggaaagtct
     1561 cccaggctcc ggtacaactc cgcctgataa ttgagcaccg tggccagaga aggatgctcc
     1621 ggtccccact cctcctcgaa caaacgcacg gctctttcat agagcggttc agcctgctca
     1681 tacttaccct cggcatgata gagcatggcc aggttatata ggttgttgcc gatggaggga
     1741 tgttccgccc ccagacaggc ttcacgctta gccagagccc tctgaaatag gggctcggcc
     1801 tgtgcatatt tgccgtgggc ccggagaaaa tccgcgagag tctccagcat ctgtgccaac
     1861 tccagaggct ccccgtgatc tagatcttca ttttcataaa tagtaagagc gcgccgcaaa
     1921 agcggctcca cagaggaata acgcccctga gcccggtaga cggcaccaag cttggtcaaa
     1981 cactgggccg cccgcatgga atcagcccca tacatactct cacacagggc aagagcctgt
     2041 ttggcaaagc tctccgcctc tttcaagttg cccatcaccc ggtaaagttc agccataccg
     2101 gcaaaaatct gagccagacc gtcttccacc cgcatctgct cgggactatt atcagacgga
     2161 gccttgaccc gcctggccag tccgggcttg gccgactgtt catcatcttc agcgcttgcc
     2221 tgggcaagcc cgcccgaatg cttctttcca gaagcgacct ggggggaact gctctccaac
     2281 tcctctgaaa cagccaccac cacgccgtcc atctcatcgc cgcgcaagcg ggccagttcc
     2341 cgcctggctt cgatcaaagc cttttgaaag agcttttccg cgtcttccag ccgggaaagg
     2401 tcataagcct ttactcccgc cgttaaatag gtctcccagc tgatgctctc ttttgccgca
     2461 tccatctcca tctctcttct tctttaagcc ccccggcaca atagaaaaat accaccggcg
     2521 gactatattt atattcagca catcctaact ctgtcatgcc cggctcgccc cccaaatatc
     2581 agagtaaaac taagataggc aaaccaacac ttcacaatcc gtccgccaac cctatccgat
     2641 tcaaggaagt cgccgatatg ctgggtatgg aagccaggga acggtttcca aagagattta
     2701 tggtgaacga ggtaagaaaa tgacggcact gacaaagaag tggaaactct ggcccctgac
     2761 cttcgacaag aaggccttca cccccagacg cattatgatc gacctggtca aactctacct
     2821 cttctatgtg ttcctatcta tgctcatcat catgggcttg aaagaccgcc tcattctctt
     2881 ccccatgcaa gataccagtt ggaagcccgg tctcaaaggt atggacgaag cactcaaatg
     2941 caccgagtcc aaagaagtaa gcttcaaaac agccgacggc aagaccctga gcggctacct
     3001 ggtgaagaac aaaaatgccg ccgccaagtt ggtcatcata aatcacggca atgccggcaa
     3061 tatcggtcac cgcatgggta ttgccgctac cctggcagca gcaggtgcct cagccctggc
     3121 ttacgattat cagggctatg gcgaatccca gggtgacccc aagctggcta atctaatacc
     3181 cgacgccacc gccgcctacg atttcgccac caaggaactg ggctacaaag ccgacgatat
//
LOCUS       DAOQNZ010000295         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14922_length_3238_cov_3.713164, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000295 DAOQNZ010000000
VERSION     DAOQNZ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14922_length_3238_cov_3.713164"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1685
                     /locus_tag="PKW73_06585"
     CDS             333..1685
                     /locus_tag="PKW73_06585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA72984.1"
                     /translation="MSKPELELAEGQPKTLPTTERTAYRWVILGFAFFITLVNYLDRT
                     AISYAMEPMKAEFGFNHEDFGKITAAFGVGYMIMTTGGGIMVDKWGARKVWAGAAIVW
                     SLCTAFIGKASTLPLFCGLRTCLGLSEGPHFPSLTRVVADWLPTQERARSTAIGLAAV
                     PMASAIGAPLLTAMMGTYGWKEMFVILGVVGIVWAVVWWIVFRDYPEHSRFVSDSELA
                     HIRDGATVDRTREHKEIRAHEISLGKTTWKFMLTNKSLVANNFAFFAFGYLLFFAVHW
                     LPEYLCYTFHVKMATVGKTLWIPWLVAASMLSIAGYISDLLWVKTGSMRIARSHMIWI
                     CQLLSAISFVPILYSPSFEWAIFFLSLGLGFGLMPNASFYAINTDLAKDKAATSLGLM
                     DSFLALAGIIAPYMTGLLVEKTKSFNSPFMLLIFFTLAGVLAVAVMQNPDRDRKLKVS
                     "
     gene            1731..>3238
                     /locus_tag="PKW73_06590"
     CDS             1731..>3238
                     /locus_tag="PKW73_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNA72985.1"
                     /translation="MTASDAERLFQPLTGKQAKIQVESLSLLENGFSNSNYRVELKSE
                     IFGRRHFVLRLLEHGTLSDLRKEEKLNQMLKATVPMPEFYLASDDNDVFSSVPYIIME
                     YVQGESLDQVNNLGQDETLALAENLGQTLAALHQMRFASQGFLDENLLVDKVVEINGQ
                     GLRRFAENILLEKGRTALLGKSLSRRFLAFLEQEADLLDDYKDLPCLCHSDFGSSNLL
                     ISGGPSPRVVAVLDFEFAFSGTPYVDLGNLLRPPFSQEKAVREAVFQGYRAGGGKLSN
                     QELYQKCLLTDLFAWLEFLGRAEVPEAVLASVRSRIISTMTDWLFLKGVLELSRLRCS
                     RERNHVTNIAHAGTKLHGTLQPKTETGMRNGAETTQIKVPPVFSRIEMAIEHAPLQHI
                     KALFPLTAADDLTHFRHQHVHGSYRLIIVIETHVEGFDVFGIVINNDRLLELLLGQIA
                     LMFRLQIDSPGNGKLEGLAALEEDIDGLGIRNSLKGTAHNTLQSLYHGFVYPL"
BASE COUNT          765 a          891 c          827 g          755 t
ORIGIN      
        1 cccggaggca gatcaagagc caggggcggc aattcctgat cgaaggcagc cccttcctgg
       61 gcaagaacag gctgattgat agcaagaggc aaaaccagag ccacagctcc ggcaaatgaa
      121 aatagggatt tgcggctgat actcattgtt aattcactcc cggcgataaa attgggctac
      181 caaatataga aacgtcacca aggtccaaaa agttcaattt ttcagaaaaa aatttttgca
      241 caggaaagag ggcgcttaca taggataagc ttatgtcccg caccggtttc ccaccgggcg
      301 agtaaaaccg cgcatcatat taaggttatc taatgagcaa acctgagctt gagctggccg
      361 aaggccagcc aaaaactttg cccaccacgg aaagaactgc ctaccgctgg gtcattctcg
      421 gttttgcctt ctttataact ctggtcaact accttgaccg cacagccatc tcctatgcca
      481 tggaaccaat gaaagccgaa ttcggcttca accatgaaga tttcggcaag attacagccg
      541 ccttcggtgt gggctacatg attatgacca caggcggcgg catcatggtg gacaaatggg
      601 gggcgagaaa ggtctgggcc ggagcggcca tagtctggtc cttatgcacg gccttcatcg
      661 gcaaggcttc cacattgccg ctcttctgcg ggctgcgcac ctgcctgggt ctttccgaag
      721 gtccccattt tccctcgctc acaagggtgg tggcagactg gctgccgacc caggaacggg
      781 cccgttccac agccatcggt ttggcggcgg tgccgatggc atcggccata ggcgcaccgc
      841 ttctgacagc catgatgggc acttacggct ggaaagaaat gttcgtgatt ctgggggtgg
      901 tgggtatcgt ctgggccgtt gtctggtgga ttgtctttag agattatccc gaacattctc
      961 gctttgtctc tgacagtgag ctggctcata tcagagacgg cgccaccgtg gatagaactc
     1021 gggaacacaa agaaataaga gcccatgaga tttccctcgg aaagaccacc tggaagttta
     1081 tgctcaccaa taagtctctg gtggccaata actttgcctt tttcgccttc ggctatctcc
     1141 tcttttttgc cgtacactgg ctgcctgagt atctttgcta taccttccac gtaaaaatgg
     1201 ccacagtcgg caagacgctc tggataccgt ggttggtggc ggcatccatg ctttccatcg
     1261 ccggctatat ctcagaccta ctctgggtta aaacaggcag tatgcgtatt gcacgttcgc
     1321 acatgatctg gatctgccag cttctttcgg caatttcttt tgtgccgatc ctctatagcc
     1381 cctccttcga atgggccatc ttctttctct ccctcggcct tggcttcgga ctcatgccca
     1441 acgcctcctt ttatgccatc aataccgatc tagccaaaga caaagccgct accagtctgg
     1501 gcttgatgga cagcttcctg gcgctggccg gcattattgc accttacatg accgggcttc
     1561 tggtagaaaa aaccaagagc ttcaactcgc cattcatgtt gctcatattc ttcaccctgg
     1621 ccggagtact ggctgtggcc gtgatgcaaa acccggaccg cgacagaaag ctgaaggtaa
     1681 gctgattgaa agaagggtgg agcagatcaa gcctgacgcc aaggttgtcc ttgactgcaa
     1741 gcgatgcgga aagactcttt caaccactga caggcaagca ggcgaaaatt caggtagaaa
     1801 gcctttctct tttagagaac ggtttttcca actccaacta tcgagtagaa cttaagagcg
     1861 aaatctttgg acgccgtcac tttgtgctgc ggctgcttga acacggcact ttgagcgact
     1921 tacggaaaga agagaagctc aatcagatgc tcaaggccac cgtacccatg cccgagttct
     1981 acctggccag cgacgacaat gatgtgttca gcagtgtgcc ttacatcatc atggaatacg
     2041 tacaaggcga gagcctggac caggtgaaca acctggggca ggacgagact cttgccctgg
     2101 ccgagaatct cggtcagacc ctggccgctt tgcaccaaat gcgttttgcc agccagggct
     2161 tcctggacga gaacctgctc gtagacaaag tggtggagat aaacggacag ggactgcggc
     2221 gctttgccga gaacattctt ctggagaaag gtcgaaccgc tctcctgggc aagtccttga
     2281 gccggcgctt cctggccttt ttggagcagg aagccgatct tctcgatgac tataaagatt
     2341 taccctgcct ctgccacagc gacttcggca gctccaacct gctcatctcc ggcggacctt
     2401 caccacgggt agtggcggta ctggactttg aattcgcctt cagcggcaca ccctatgtag
     2461 acttaggcaa tctactgcgc cccccgttct cccaggagaa agcagtgaga gaagctgttt
     2521 ttcagggcta ccgggcaggc ggcggcaagc tcagcaatca ggagctttat caaaagtgcc
     2581 tgctcactga tctctttgcc tggctggaat ttctcggccg cgcggaagta ccggaagcgg
     2641 ttctggcctc agttcgaagc cgcatcatta gcaccatgac cgactggctc ttcctcaaag
     2701 gagttttaga actcagccgt cttaggtgct ctcgggaacg gaatcacgtc acgaatattg
     2761 cccatgccgg tacaaaattg cacggtacgc tccagcccaa gaccgaaacc ggcatgagga
     2821 acggtgccga aacgacgcag atcaaggtac caccagtatt cagccggatc gagatggcta
     2881 tcgagcatgc gccgctccag cacatcaagg cgctcttccc tctgactgcc gccgatgatc
     2941 tcacccactt tcggcaccag cacgtccatg gctcttaccg tcttattatc gtcattgaga
     3001 cgcatgtaga aggctttgat gtctttggga tagtcataaa caatgacagg cttcttgaac
     3061 ttcttctcgg tcagatagcg ctcatgttca gattgcagat cgattcccca ggaaacggga
     3121 aactcgaagg tctcgccgct cttgaggagg atatcgacgg cctcggtata agaaactctc
     3181 tcaaaggaac tgctcacaat accctgcaga gtctctatca cggttttgtc tatccgct
//
LOCUS       DAOQNZ010000296         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14933_length_3237_cov_3.302011, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000296 DAOQNZ010000000
VERSION     DAOQNZ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14933_length_3237_cov_3.302011"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKW73_06595"
     CDS             complement(<1..454)
                     /locus_tag="PKW73_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HNA72986.1"
                     /translation="MHDDFSRTPVASDHLRPWWYLLIIEMGVMISIPTFVLGGQLGLG
                     MSFSDLIPATFCGALILGVLGACTAHLGAVTRCSTALISRLTFGARGSAAIALLLALG
                     ITGWWGVQVEMFADAVVQLARNGFSLSLPRELVILAGGMAMITTAALGI"
     gene            complement(571..1008)
                     /locus_tag="PKW73_06600"
     CDS             complement(571..1008)
                     /locus_tag="PKW73_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72987.1"
                     /translation="MQLCDESTTFEGTVDTVWQLLVDWQNMPSWDIFMESLDFDGPLK
                     MGSRGLLKMKNGPTAPLLVTAFNPPYSYTDELTFWFTRLIFHHELTELEAGKIRVRFV
                     VEGEGLLPLFVGSLMKNDMRKKMPVLLDNFQKQYEIRRQKHQI"
     gene            1237..1755
                     /locus_tag="PKW73_06605"
     CDS             1237..1755
                     /locus_tag="PKW73_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72988.1"
                     /translation="MNKVLSFIKNNASLTVMASLVVICVFSMRFQETKPTAKNLEVSA
                     ILLGVDGTTANFELTVKNADTSPFIYKKAIEATGMPSRHFKAEIATGNVVRTSLNKGW
                     DELPFKIPEDQIFMTSKINDGLGNSNQVEIAPGATAGSTISLENMPHGGSTVVFTVDG
                     GKKSQPLSVSVP"
     gene            complement(1774..3018)
                     /locus_tag="PKW73_06610"
     CDS             complement(1774..3018)
                     /locus_tag="PKW73_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA72989.1"
                     /translation="MLLRTPILRSNLTLINRSLTVLVLAVLNGLPTMAPAAANPDLTP
                     DQREEVKLKSEKWGILRQKAEKLSRENKKEEALVIYEQLLAERKALGLDLAAEYALLG
                     CVLTELGRQEEAVKVYKDMLAARESLNGKDDPQVIYALDEYAKSLEHFGKKGEAGQLR
                     SRIARIQKETAARPVFGKITSAPGSAARLKEAEQVNSRGEQLVKADLQDKAYLYFDRA
                     VQLNPSDPRFLRNRAETLSWKQDFAKAMADLNKAIKLKPDFARAYVDRAFVHQNLKQY
                     KQAIADFEKAYTLDGKDIDAMGSRAKLLDEMGKHEEAVAGYTRVIQTDPSQYWPYIQR
                     SVAYASMKRFKEAEDDLTVLINRAPGDTDYYEYRAGVYIKSGDLKKALNDYNKMIELS
                     PQNSYFKEKRAALTKQLNGQTK"
BASE COUNT          728 a          835 c          834 g          840 t
ORIGIN      
        1 tgatgcccag ggcggcagtg gtgatcatgg ccatgccgcc tgctaagata accagctccc
       61 tcggtaggga gagcgaaaac ccattacggg ccaactgcac gactgcatcg gcaaacatct
      121 caacctgcac tccccaccag ccggtgatgc ccagggccaa aagcaaggcg atagcggcag
      181 agcctctagc gccaaaggtg agacgcgaaa ttagagcggt cgaacatctg gtcaccgctc
      241 ccaggtgggc ggtgcaggcg cccagaacac ccaggatcag cgctccgcaa aaggtggccg
      301 gaatcaggtc ggagaagctc atacccagac cgagctgtcc acccagtaca aatgtgggaa
      361 tgcttatcat cacacccatt tcaataatga gtagatacca ccagggtctc agatgatcac
      421 ttgctaccgg cgttctggaa aaatcgtcgt gcattcctct tgctccccct ctttctgtgt
      481 gccgagggta acatggtctc aacgagctgg gaatgctaaa aatacgctgg ctgtgccggt
      541 aaacactatc cttctgtggc ggaactgtgg ctaaatctgg tgcttctgcc ggcgaatttc
      601 gtactgcttc tggaagttat cgagcaacac aggcatcttc tttcgcatat cattcttcat
      661 caaggaacca acaaatagag gcaggaggcc ttctccttcc accacaaatc taactctaat
      721 tttccccgcc tcaagctcgg tcagctcatg atggaaaatg aggcgagtga accagaatgt
      781 cagttcgtct gtgtatgaat agggtgggtt gaatgcagtc accagaagtg gagccgtcgg
      841 tccgttcttc atcttgagaa gaccgcgact gcccatctta agtggaccgt caaagtcaag
      901 actttccata aagatgtccc aggatggcat gttctgccag tccaccagaa gttgccagac
      961 ggtatcgacg gtgccttcga atgtagtgct ttcatcacaa agttgcatag aggataattc
     1021 tacctgacaa gaccgcaggc ttgacttcta aaatgggaaa acgtagccgg tgcattgctt
     1081 tgtagttcaa gaaggtaacc gtctcccggg taagcattac acttgccgct tgcctcctcc
     1141 acaagtgaga ggctcgctct ctagtcaggg cttgtcaatc tggctatagt agttagtgtt
     1201 tgtctgtaag tacagcgctt ccaggggatg tctttcatga acaaggttct cagtttcatc
     1261 aagaataatg cgtcgttgac ggttatggca tcactggtgg ttatatgcgt tttttccatg
     1321 cgctttcaag aaacaaaacc caccgctaag aatcttgagg taagcgcgat tctcctgggc
     1381 gtggatggta cgaccgctaa tttcgagctg actgtcaaga atgccgacac atcccctttt
     1441 atctataaaa aagcaatcga ggccaccggt atgccgtccc gccattttaa ggcggagatt
     1501 gctaccggta atgtcgtccg cacctccctc aataagggct gggatgagtt acccttcaag
     1561 attcctgaag atcaaatttt catgaccagt aaaatcaacg acggactggg taattcaaat
     1621 caagttgaga tagcgcctgg tgctaccgcc ggtagtacca tatctcttga aaatatgccc
     1681 catggcggct cgacggtggt cttcaccgtc gacggcggca agaagtctca accgctatcg
     1741 gtcagtgttc cctgaagcaa cctgcgcacg tgactacttg gtctggccgt tcagttgttt
     1801 tgtcagggcg gcgcgtttct ctttaaagta tgagttctgc gggctcagtt caatcatttt
     1861 gttgtagtcg ttcaacgcct tcttcaaatc accggatttg atataaaccc cggcccgata
     1921 ttcatagtag tcggtatcgc cgggggcgcg attgattaga acggtgaggt cgtcttccgc
     1981 ttctttgaag cgtttcatcg aggcatatgc cacggatctt tgaatgtaag gccagtactg
     2041 gcttggatca gtctggatga ctctggtgta gccggccacc gcttcttcat gtttgcccat
     2101 ttcatccaaa agctttgctc ttgagcccat ggcatcaata tctttgccgt ctagggtgta
     2161 ggctttttcg aaatcagcaa tggcctgctt gtactgtttg agattctggt gcacaaaggc
     2221 tctatccaca taggctctgg cgaaatcagg tttgagtttg atggctttat tgaggtcggc
     2281 catggctttt gcaaagtctt gcttccagga gagagtttcg gcccgatttc taaggaaacg
     2341 cgggtcactg gggtttaact gcacggccct atcaaagtag aggtaggctt tgtcctgcaa
     2401 gtcggctttc accaactgct ccccacggct gtttacttgc tcggcctcct tcaaacgtgc
     2461 cgccgatccc ggcgcgctgg tgattttgcc gaatacgggg cgggcggcag tctctttttg
     2521 aattctggca atgcggctgc ggagctggcc ggcttcccct ttcttgccga aatgctcaag
     2581 tgatttggcg tattcatcca gggcatagat gacctgcggg tcgtctttgc cgttcaggct
     2641 ttccctggcg gcgagcatgt ctttataaac cttgactgct tcttcttgtc ggccaagctc
     2701 ggtcaatacg cagcccagaa gggcgtattc agcggccagg tcgagaccga gggctttgcg
     2761 ctcagccaga agttgttcat aaatgaccag ggcctcttcc ttcttgtttt cgcggctgag
     2821 cttctcggcc ttctgtctga gaattcccca cttttcactc ttaagcttta cttcttcccg
     2881 ttgatcaggg gtcaggtcag ggttagcggc ggccggagcc atggtcggaa gaccattcag
     2941 gactgccagg accaggaccg taaggcttct gtttatgagt gttaaattgc ttcgcaagat
     3001 gggggtcctt aataacatgt actcgctcca gggcattttg gcatagaata agatgagtcg
     3061 ggcaaattgg tagcttaaac catgcaatca gcgcaatctc ctctccatat ggtcatacag
     3121 gagcctgtgc tgcaccagtt tatcgccttt ctggccgcca aaaaggctga acaggaggcc
     3181 agtccgggtt ctcatgtgcg gattgaactg cccagagatt ttatcatccg cgtccgg
//
LOCUS       DAOQNZ010000297         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14935_length_3237_cov_3.263042, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000297 DAOQNZ010000000
VERSION     DAOQNZ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14935_length_3237_cov_3.263042"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..2100
                     /locus_tag="PKW73_06615"
     CDS             118..2100
                     /locus_tag="PKW73_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA72990.1"
                     /translation="MAKGVAPYLLLACAVALSLSGCIAPPPEPGTRISDNLRVAELKH
                     PRTEQENSVQNSAPKATLSSDNIAADAEFPNPGPFKLVTDKDGNELWQARGELGKFGG
                     TLKLTSFGDGPKTFNCWDASDVESHGIGLMQFDSLVDIDPWTGKATPKLCKTVDVSPD
                     GRVVTFTLRKGLKWSDGKPLTSDDVVFTFDTIVKDGWGEGSSKDTISIPTDYPVIKKV
                     DDLTTTFTFKKPFSPFLLNLNAVTIAPRHVLLPVTKKGKAAFRPFWNVNVDPKTIVGS
                     GPYILSQYIPAQRIEFKRNPNYHMVDKEGRRLPYLDKIVISIVKEQPQMILKFLGGEV
                     DFLDARSVRGMDASVLKQKEKEGNFTLRNLGPDDGTVFLMFNMCRRKDPKTGKFYVDP
                     VKQDWFNSLNFRKAVSHSIDRPSIVNNILRSVGYPLYTCQTTAGVYHNTDLKPITYSL
                     EEAKKLLKEDGFVLKGDELFDKKGNRVEFDLITNAGNTIRDAVCIHIKEQLKLLGIKV
                     NYAPIEFNAMINRTHTTLDWQAMMLGLSGSRLEPYSGANIWKSDGRMHEFDQRLPDEK
                     DQLKVTDARPWELELDKCLDTAAGSFDEKVRAENYKRAEAIAYEQEPLIFIYTNAVLT
                     AARNTMGNYKPTPLAIYYTPKGSLHNLEEIYLKEGK"
     gene            2100..3068
                     /locus_tag="PKW73_06620"
     CDS             2100..3068
                     /locus_tag="PKW73_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA72991.1"
                     /translation="MSTFLYIIKRFIQAVPLLFIISIIAFTLLKCTPIDPLASMRANP
                     SVSRSAIKAEEERLGLNKPAPVQYFYWAGNALQGNLGISSTGAPVIERLTAHAGNTLM
                     LNILVILLTWTIALPSGIYAAVHRAQFIDKVFGILSAIGMSMPAFLLAFLLMVLALVT
                     GLLPIGGLTSADFGELSFFDQVIDIAHHLIIPVTVLTCISLAGIQRQVRSNLLDVLRA
                     DYVRTARAKGLPENRVIYKHALRNAINPLITILGFEFAGLLSGAALTEMVLSYPGLGK
                     LTLDAMLTKDMNMVMASIMMGAIMLVTGNLFADVLLKFTDPRISHD"
BASE COUNT          798 a          923 c          782 g          734 t
ORIGIN      
        1 actgccgcgc ccaagcaaat agtcggtgcc agaaacactc tgcacaacta cgatccgacc
       61 ccgctttcac agctcggcgt aggtttgcat aacttgccgg agatctgggt caagtaaatg
      121 gcaaaaggcg tcgctcccta tcttttgctg gcctgcgcgg tggctctcag cttgagcggc
      181 tgcatcgcac caccacctga gccgggcact agaattagcg acaacttgcg ggtggctgag
      241 ctgaagcacc ccaggactga gcaagaaaat tccgtacaaa attccgcccc caaagccacg
      301 ctttcatcgg acaacatagc tgccgatgcg gaatttccta atccgggacc atttaaattg
      361 gtcacagaca aagacggcaa tgagctctgg caagcccgag gagagctggg aaaattcggc
      421 ggcaccttga agctgacttc ctttggcgac gggcctaaga cttttaactg ctgggacgcc
      481 agcgacgtgg aaagccacgg catcggactg atgcaattcg attctctcgt ggacatcgac
      541 ccctggaccg gcaaagccac acccaaactc tgcaaaaccg tggatgtcag cccggacgga
      601 cgggtagtca ccttcactct gcgcaagggg ctgaagtggt ccgacggtaa gccgctcact
      661 tcggacgatg tggtttttac ttttgatact atcgtcaaag acggctgggg ggaaggcagc
      721 agcaaagata ctatttccat ccccacagac tatccggtga tcaagaaggt ggacgatctg
      781 accaccacct tcactttcaa aaaacctttc tcaccctttc ttctaaatct aaacgccgtc
      841 accatcgccc ccaggcatgt gctcttaccg gttacgaaaa agggtaaggc agccttcagg
      901 cctttctgga acgtcaatgt agatcctaaa acgattgtgg gctccggtcc ctatatactc
      961 tcgcagtata ttcccgccca gcgcatagaa ttcaaacgca atcccaacta ccacatggtc
     1021 gacaaagaag ggcgaaggct gccctacctg gataagattg tcatctccat cgtgaaagaa
     1081 cagccccaga tgatcctcaa gtttctcggg ggtgaggtgg attttcttga cgcgcgttcc
     1141 gtcagaggca tggatgcttc cgtcttgaag cagaaggaaa aggaaggcaa cttcaccctg
     1201 cgcaatttgg gacccgatga cgggacggtt tttctgatgt tcaatatgtg ccgtcgcaaa
     1261 gatcctaaga ccggaaaatt ttacgtggac ccggttaaac aggactggtt caacagcctc
     1321 aattttcgca aggcggtcag ccattccatc gatagaccga gcatcgtcaa caatatcttg
     1381 cgttcggtgg ggtaccccct ctatacctgt cagaccaccg ccggtgtcta tcataatacc
     1441 gacttgaaac cgattactta cagtcttgag gaagcgaaga aactgctcaa ggaagacggt
     1501 ttcgtcctta agggcgacga gctcttcgac aagaaaggca accgggtgga gttcgatctc
     1561 atcaccaatg ccggtaatac catccgcgat gcggtctgca tacacatcaa agaacaattg
     1621 aagttgctcg gcattaaggt caattatgcc cccattgaat tcaatgccat gattaaccgt
     1681 acccatacca cgttagactg gcaagctatg atgcttggtc tttccggcag tcgcctggag
     1741 ccctattcgg gcgccaatat ctggaagtct gatggacgca tgcatgaatt cgaccaacgc
     1801 ctgcccgatg aaaaagatca gctcaaagta accgatgccc gcccctggga gttggaacta
     1861 gacaaatgcc ttgataccgc cgccggtagc ttcgacgaaa aggtgcgagc cgaaaactac
     1921 aaacgagccg aagccattgc ttacgagcaa gaacccctga tattcattta tactaatgcc
     1981 gttttgacgg cggccagaaa caccatgggc aactacaaac cgacaccgct ggcaatttat
     2041 tacacgccca agggcagctt gcacaacctg gaagagatct atttgaagga ggggaaataa
     2101 tgtccacctt cctctatatc atcaagcgct tcatccaggc cgtgccactc ctgttcataa
     2161 tttccatcat tgcctttact ctgctcaagt gtacgcctat cgatccactg gccagcatgc
     2221 gcgccaatcc ctcggtttct cgctcggcaa tcaaggcgga agaagaacgc ctcggtctca
     2281 ataaaccggc tcccgtgcag tacttttact gggccggcaa cgcgctccag ggcaacctcg
     2341 gtatttccag caccggcgcg ccggttatag aaagactgac cgcccatgcc ggcaacactc
     2401 tcatgctcaa tattctggtg attctgctca cctggactat tgccttacca tccggaatct
     2461 acgcagccgt gcacagggct cagtttatag ataaagtctt tggtattttg agcgccatcg
     2521 gtatgtctat gccggctttc ctactggcct tcttgctcat ggtgctggcc ctggtcaccg
     2581 ggcttttgcc tataggcggc ttgaccagcg cggattttgg cgaattgagc ttcttcgatc
     2641 aggtgatcga tatcgcccac cacctgatca taccggtgac ggtgctgacc tgcatttccc
     2701 tggccggcat acaaagacag gtacgcagta acttgctcga tgtactcagg gccgactacg
     2761 tgcgcacggc cagggccaaa ggtctgcctg aaaatcgggt tatatataaa cacgccctgc
     2821 gcaatgccat caaccccctc attaccattc tcggttttga atttgccggg cttttgagcg
     2881 gagcggccct caccgagatg gtgctctctt atccgggtct cggtaagctt accctcgacg
     2941 ccatgctcac caaagacatg aatatggtca tggcttctat catgatggga gccatcatgc
     3001 ttgtcaccgg caacctcttc gccgatgtct tactcaaatt caccgacccg cgcatttccc
     3061 acgactgagg atagatggca atggccgccc aagcaccagc tctagcatct acaaaagtca
     3121 gtcgccgcac tgtcttcaaa gggctatgga aagacaatgt agccagagca gcgcttatag
     3181 tgctggcctg tttttatgcc gcagccgcac ttgcagatct tatcaccccc tatggca
//
LOCUS       DAOQNZ010000298         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14947_length_3235_cov_3.847484, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000298 DAOQNZ010000000
VERSION     DAOQNZ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14947_length_3235_cov_3.847484"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="PKW73_06625"
     CDS             <1..1400
                     /locus_tag="PKW73_06625"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNA72992.1"
                     /translation="LPSGGYARINLLDVDMSRSPVKIRPVTGSYAFAKLKDVREHCRD
                     SGALAAINANYFKTTNGIPLGTLIEDGDWLAGPLYDRVTLGFTEGGYARIDRVSLHGL
                     LYTDNTENPVLWINNVNQPRRTGSHCILYTRHWGGSASLPYPGVLVGVDASGRVVSTD
                     DLKLTIPYGGFVLADSKGSPISKLQEGEQVNIRWQIHPGDWADVTEAVSGGPLLLKNG
                     KLDLDLKREKFPASFVGSHIHSRTACGITADDHLLLATFEGPHSMYDIAKFFLKRGCT
                     EAMNLDGGGSTTMVVDGKTVTANANHAQRRVAVALGLFSPEKARALSTVLGGSYRPTQ
                     EMASFVGTSIVPYEKVYKEAIRQPEADPLMTSLMSFDLRQQSDEVRSAAVSQPQPLDD
                     AAVLEQSLAQQEPQAAVSGSTFPISEPSPTRSAGTGQLSQVESKMPAGSAKLKSVKPR
                     FGKAPVSLLKGLWNR"
     gene            1439..2395
                     /locus_tag="PKW73_06630"
     CDS             1439..2395
                     /locus_tag="PKW73_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNA72993.1"
                     /translation="MGDKFAVGVDLGGTKILGSVVNLNTGRMVSTSKKKTRVVNEGLD
                     LVKRISLVVADAIDESGMDPSKLAGIGIGAAGMVDREKGILLAAANLGANDVVLAEPL
                     KAQFGLPVKVGNDVEVATLGELNFGAGRECNNFVCIFVGTGIGSGIVHEGRILRGGSG
                     TAGEVGHIIVDNDGLVCGCGGYGCLETYASRTAVAKSIVYDLGRGMDSVLRDKIDPQK
                     GILRSKAISQAVECGDRVALRAIHKAARYMGLGLATVINFYNPKRIIIGGGLVEAVPL
                     FLELAEEEARRRCLRIPAKKTEIVQAELGDFAGTIGAALLVR"
     gene            complement(2388..>3235)
                     /locus_tag="PKW73_06635"
     CDS             complement(2388..>3235)
                     /locus_tag="PKW73_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNA72994.1"
                     /translation="GFTCPNRDGTRLFGGCTFCDERGSGAPTIKTALSIRNQLQSGMA
                     RIRKRFKAGKFLAYFQAFTNTYAPEGVLKELYDVALEPEDVVGLAVGTRPDCLDDNIL
                     ALLKTYHEKTFLFLEVGVQTIHNRTLEAINRGHTGEEFFDAVERAKKHGLRLATHLIF
                     GLPGESEADMMETVRRVAPLGLTAIKIHQLCIYKGTPMEEDYRAGRLQTMEEDTYVRL
                     VADALEMLPPDLVIMRLVAEGSQDEIIAPSWAFEKERVMQKIEAELNRRGSKQGSRFA
                     GAALI"
BASE COUNT          674 a          869 c          912 g          780 t
ORIGIN      
        1 gcttgccttc gggcggctat gccagaatca atttgctcga tgtcgatatg tcccgctcgc
       61 cggtgaaaat tcgtccagtt actggttctt acgcctttgc caaactcaag gatgtgcgcg
      121 agcactgccg tgacagtggt gctcttgctg cgatcaatgc caattatttt aaaactacca
      181 acggcattcc tctgggcact ctcattgaag acggtgattg gcttgccgga ccgctttatg
      241 acagagttac tctcggcttt accgagggcg gctatgcgcg catcgataga gtttcattgc
      301 acggtctgct ctataccgat aacaccgaaa atccggttct ctggataaac aacgtcaatc
      361 agccgcgccg caccggttcc cattgcatcc tttacacgcg ccattggggc ggcagtgcca
      421 gtttgcctta tcccggagtg ctcgtcgggg tggatgcttc ggggcgggtc gtatctactg
      481 atgacttgaa gttgacgatt ccttatggcg gttttgtgct ggccgattcc aaaggctcgc
      541 ccatcagtaa gctgcaagaa ggggagcagg tcaatattcg ctggcagatt catcccggtg
      601 attgggctga cgtaaccgag gcggtgagcg gtggtccgct tctgctcaaa aacggcaaac
      661 tcgatctcga tttaaagaga gaaaaatttc ccgcttcctt tgtcggcagt catatccatt
      721 cccgcactgc ctgtggtatc accgccgacg accatttgct gctcgctact ttcgaaggtc
      781 ctcacagtat gtacgacatc gccaagtttt tcttgaagcg tggttgtacc gaagctatga
      841 atcttgacgg tggaggttcc accactatgg tggtagacgg caagactgtg actgccaatg
      901 ccaaccatgc tcaaaggcgc gtcgccgttg cccttggttt gttctcaccg gagaaagccc
      961 gcgccctctc tactgttctg ggaggctcct atagacctac ccaggagatg gccagctttg
     1021 taggtacgag tatcgtgcct tacgagaaag tctacaaaga agcaatccga caacctgaag
     1081 ccgacccgct tatgacctca cttatgtcct tcgacctcag gcagcaatcc gatgaggtgc
     1141 gcagcgctgc cgtcagtcaa ccgcagcccc tcgatgatgc ggctgtgctt gagcagtctt
     1201 tggctcagca agagccccag gcggctgtct ccggctccac attccccata agcgagccct
     1261 cgccgacgcg gagcgcaggc accgggcaat taagccaggt cgagagcaaa atgccggccg
     1321 gatctgctaa attgaagtct gtcaaaccca ggtttggaaa ggctccggtc agccttctca
     1381 aggggctgtg gaaccgctag cctgagtttg ctggtacatt tgcataggcg gactggtctt
     1441 gggcgataaa tttgcagttg gcgtagatct tggcggaacc aagattcttg gttcagtagt
     1501 caatctcaat acgggccgta tggtctccac ctccaagaaa aagacgcggg tggtgaatga
     1561 aggactggat ctagtcaagc gtatttccct ggttgtggcg gatgccatcg atgaatcagg
     1621 catggaccct agcaaattgg ctggtatcgg tattggtgcg gcgggcatgg tcgaccgcga
     1681 aaagggtatt ctcctggcgg cggccaattt gggcgccaat gatgtcgttt tggcggagcc
     1741 gctcaaagcg caattcggtc tacctgtgaa ggtgggcaat gatgtggaag tggccaccct
     1801 gggtgaactc aatttcggtg ccggccggga gtgcaataac tttgtctgta tctttgttgg
     1861 aaccggcatc ggctccggca ttgtgcatga ggggcggata ttgcgcggcg gcagcggtac
     1921 tgccggcgag gtcgggcata tcattgtaga taacgatgga ctcgtctgcg gctgcggtgg
     1981 ctatggctgc ctggagacct atgcgtccag aacggctgtg gctaagtcta tcgtctacga
     2041 tctggggcgc ggcatggatt ccgtcttgcg cgataagatc gatccgcaga agggtatctt
     2101 gcgctccaaa gctatttcgc aggctgtcga gtgcggtgac cgcgtggccc tgcgagccat
     2161 acacaaggct gcccgctata tggggcttgg tctagccacc gtaatcaatt tctataatcc
     2221 caaacgcatc attattggtg gcggtctggt ggaggctgtt ccactcttcc tggaattggc
     2281 ggaagaagag gcgcgccgcc gttgcctgcg cataccggct aagaaaactg aaatagtaca
     2341 agccgaactg ggcgatttcg ccggtactat tggcgccgcc ctcctggtta gataagggcg
     2401 gctccggcga agcgcgagcc ttgcttgctg ccgcggcgat ttagttcagc ttcaattttt
     2461 tgcatcaccc gctctttttc aaaggcccag gagggtgcaa tgatttcatc ctgggaacct
     2521 tccgccacca ggcgcatgat caccaggtcc ggcggcagca tttccagggc gtcggctacc
     2581 agtctcacat aagtatcttc ttccatggtt tgaaggcgtc cggcccggta atcttcttcc
     2641 atgggtgtgc ctttatagat gcacaactgg tgaatcttta tggcagtcag ccccagcggc
     2701 gccacgcggc gcacggtctc catcatgtcc gcttcgcttt cgcctggtag accaaaaatc
     2761 aggtgggttg ccagtctcag accatgtttc tttgcccgtt ccacagcatc aaaaaactct
     2821 tcaccggtat gacccctgtt gattgcctcc agggtgcggt tgtggatggt ctgaaccccc
     2881 acctccagaa acaagaaggt cttttcatgg taggtcttga gaagagccag aatattgtca
     2941 tccaggcaat cgggcctggt gcccacggcc agacccacca catcctcagg ttccagagcc
     3001 acgtcgtaaa gttccttcag gaccccttcc ggcgcgtaag tatttgtaaa ggcttgaaag
     3061 taggccagaa atttgccggc tttaaaacgc ttgcggatgc gagccatgcc tgattgcagt
     3121 tgattcctta tagaaagagc ggtcttgatg gtgggtgcac cggagcccct ttcatcgcaa
     3181 aatgtacagc cgccaaagag cctggttcca tcgcgattcg ggcaggtgaa gccgg
//
LOCUS       DAOQNZ010000299         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14956_length_3234_cov_4.016357, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000299 DAOQNZ010000000
VERSION     DAOQNZ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14956_length_3234_cov_4.016357"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PKW73_06640"
     CDS             complement(<1..485)
                     /locus_tag="PKW73_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA72995.1"
                     /translation="MSEPDATYNEAPDLKERPPELVEGKEAAPPQLVETTAEAEQNHA
                     GETSETDLPQFPQTAFKIVETLGQGGMGTVYRVRDEDLQVDFAVKVLRPELAKDRNLV
                     SRFKKEAEAAGELSHANLASIYKTGECLDGRPYLVMDYVPGPSLAAVLQKEGHLQAER
                     AL"
     gene            764..1606
                     /locus_tag="PKW73_06645"
     CDS             764..1606
                     /locus_tag="PKW73_06645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNA72996.1"
                     /translation="MVPLFLSFTLASLSLTASFSLTAASPVFAADSKETFKKKLGRFF
                     SFKLSQTQEEALGKEMDVNVRKQFKVSTDSLASQAVQTLMGKLAASAHPAFPIKITVL
                     EEPEIVNAFAIPGGYIYVTRGMLNLVDNDAQLAGVLGHELAHVTQNHVSQRVSNQTTA
                     NLAVRLMSRLSEKDLEKSRVTNLVKLVVFQKFSRDAEYEADRVGLDYMAQAGYNPLAM
                     AQVKDKFLAITNDKMSIEFLSSHPNTSKRKAEILALIKEKNLVKPGQITETRDFLTAL
                     GKSR"
     gene            1808..>3234
                     /locus_tag="PKW73_06650"
     CDS             1808..>3234
                     /locus_tag="PKW73_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72997.1"
                     /translation="MRIRIDRRSDQSCALFIDGDLQFDSRDERIYHECLALPALALVE
                     KRCLKPLSVLIVGGGDGLTAREVLKSSSVVRIDLVDYSQEVVSLGKEAFAALNEGSLQ
                     AKRLHLHIEDAWTYVERKLAEQATYDLIICDLTVPADLEGARFHTIEWYERLSKLAGE
                     CGLLAVNGVSPWATARAFWSIFNSMAAANLNVLPLKVQIPSFARLGYGPDWSFYLASR
                     AAIAPHEFSHPLPLATPRSVLLDGEYLHRLFRFQFPEDVLGLQSSAHPAALGSDILLH
                     YFRNGEEHMSANLFVWDALNDERCDFSSPAPDCGDSILPPEIKDKLQELLGRSDIGNV
                     SAQPVALNGSGFDTTDLDSKNAAGVRADGPISAEELFRGVTSLMPSLHRYQTKQAIAE
                     FLSRPLQFLQSIDLPALVAALLRRAAELPERLVQELRHLKERLVDFCGDYEALMQTGH
                     RVLTVVALVVIVGNLMHPEAVYGKG"
BASE COUNT          676 a          854 c          886 g          818 t
ORIGIN      
        1 agggcccgct ccgcctgcag gtgcccttcc ttttgcagaa ccgccgcaag actggggccg
       61 ggcacatagt ccatgaccag gtaggggcgg ccgtccaggc attcgccggt cttgtatatg
      121 ctggctagat tggcatgact gagttctccg gcggcttccg cctctttctt gaagcgactg
      181 accagattgc gatcctttgc cagttccggc cgcaacactt taacggcgaa gtccacctgc
      241 aggtcttcat cgcgcacacg gtaaacggtg cccatacctc cctgaccgag ggtttccact
      301 attttgaagg cagtctgggg aaattggggc aggtcggttt cggaagtttc gccggcatga
      361 ttttgctctg cctccgccgt ggtttctacc agttgaggcg gtgctgcttc cttgccttcc
      421 accagttctg ggggacgctc cttgaggtct ggcgcttcat tgtaggtggc atccggttca
      481 gacaaggtga attcctcaac ctgcgggcta catacactta taccctggtg attcatcctc
      541 aacattgctg ccatgagctg agaaaggcaa gattaacagg gaaatagcgg ggctcttaca
      601 aggtggttcc gcatacaatt tttttctttt ttattcgact tttgagttat gcttttcctg
      661 tcgcctctgt aagtcaacca gggcggcaca tcactgccag ggaggaatga gtttgagttt
      721 gtccaggcta agcactaagg ggtcggcgcg gttacggttg cctgtggtcc cgcttttctt
      781 gtcttttacc ctggcatcgc tttctcttac tgcatcattt tctcttactg ctgcgagccc
      841 tgtctttgcc gccgattcaa aggagacctt caagaaaaag ctgggcaggt ttttcagctt
      901 caagctcagc cagacccagg aggaagctct gggaaaggag atggatgtga atgtgcgcaa
      961 gcagtttaaa gtctccaccg atagtctggc atcacaggcg gtgcaaaccc tcatgggtaa
     1021 attggcggct tccgctcatc cggctttccc cataaaaatc accgtgctgg aagagccgga
     1081 gatagtaaac gcctttgcca tccccggtgg ttacatctat gtgacgaggg gtatgctcaa
     1141 cctggtggac aatgacgcgc agctcgcggg agtacttggt cacgaactag cccacgtgac
     1201 gcagaatcac gtctctcaac gggtgagcaa tcagactacc gccaatctgg cggtgcgact
     1261 catgtcccgc ctcagtgaga aagacctgga aaaatccagg gtaaccaacc tcgttaaact
     1321 cgtggttttt caaaagttca gtcgcgatgc cgagtatgaa gcagatcggg tcggactcga
     1381 ttacatggct caagccggct acaatcctct ggccatggcc caggtcaagg acaagtttct
     1441 ggcaatcaca aatgacaaga tgagcattga atttctcagc agccacccaa ataccagtaa
     1501 gcgcaaagct gagattctgg cactcatcaa agagaagaat ctggtaaaac cggggcagat
     1561 tacggagacc agggattttc tgacggccct gggaaaaagc cgctagacag gggacggttt
     1621 ttcaaaccag gggtctgtta acagttgtta atgatgctag tgcggggagc gactgaaccg
     1681 gtcgcagggg gcgctttgtc tgtctttcct tggcaacctc ctgattttat ctggcttctg
     1741 caccggcttg attgaaacgc cgctctgttt gtagcaaact tctttctctg acccaagtgg
     1801 agaagctatg cgtattcgaa ttgacagaag aagtgatcag tcttgcgccc tctttataga
     1861 tggggatttg cagttcgata gtcgagatga gcgcatttac catgaatgtc tggctttgcc
     1921 ggcccttgct ctggttgaga agcgttgcct gaagccgctt tccgtcttaa tcgtaggtgg
     1981 cggcgacggc ttgacggcga gagaagtgct caagtcttct tccgtggtta gaattgacct
     2041 ggtggattat agtcaagaag tagtgtccct tggtaaagag gcctttgctg ctttgaacga
     2101 gggcagtctt caggccaagc gtctgcacct ccacattgaa gatgcctgga cctatgtgga
     2161 aagaaagctt gccgagcagg ctacctatga cttgattatc tgcgacctga ctgttccagc
     2221 ggatcttgaa ggggctcgct ttcataccat cgagtggtac gagcgcttga gtaagcttgc
     2281 cggggaatgt ggacttctag ccgtaaacgg ggtctcaccc tgggctacgg cacgagcctt
     2341 ctggtccatt tttaacagta tggctgccgc gaacctcaat gtcttaccgc taaaagtgca
     2401 aattccaagc tttgccaggc tcggttacgg tcctgattgg tccttctacc tggcttccag
     2461 agcggccatt gcgccgcatg aattctctca ccccctgcct ctggccacgc cccgctccgt
     2521 tttactggat ggggagtatt tgcacaggct cttccgcttc cagtttcccg aggatgtgct
     2581 cggtttgcaa agcagcgccc acccggctgc tcttggtagc gacattttgc ttcactactt
     2641 ccgcaacggc gaagaacata tgtcggccaa tctctttgtc tgggatgccc tcaatgatga
     2701 gcgctgcgac ttttcctcgc cggcgcctga ttgcggtgat tcgatcctgc ctccggaaat
     2761 caaagataaa ctgcaggaat tactcggtcg gtccgatatc ggcaatgtct ctgctcagcc
     2821 agtcgctttg aacggaagtg gcttcgatac gaccgatcta gatagcaaaa atgcagctgg
     2881 ggtgcgggcg gatggtccaa tcagcgctga ggaactcttc cgcggtgtca ccagcttgat
     2941 gccctctttg caccgctatc agaccaagca agcgattgct gaatttttga gtcgccccct
     3001 gcaatttctt caatccattg atttgcccgc cctggtggcc gccctcttgc ggcgcgctgc
     3061 cgaactaccg gagcggctgg tgcaagagtt gcgccatctg aaggagcgcc tggtagattt
     3121 ttgcggcgac tacgaggccc ttatgcagac cgggcaccgg gtcttgactg tggtggcgct
     3181 ggtggtgata gtcggtaacc tcatgcatcc cgaagcggtg tacggcaagg gtga
//
LOCUS       DAOQNZ010000300         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_14984_length_3231_cov_2.994647, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000300 DAOQNZ010000000
VERSION     DAOQNZ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14984_length_3231_cov_2.994647"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKW73_06655"
     CDS             <1..464
                     /locus_tag="PKW73_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA72998.1"
                     /translation="LAGQRAFAEFAAAAKDEGWLSAGSWFYRLAALGEYQNKLALNVP
                     EKTAFRLPLPLALPSLSDQDESCLRIEMVKTTGAGDGDVNLLILNKEGQVVETLALAG
                     SNGEKTCRLSGLTCLARPDVADGGKAALYFEPGNYLLKGIYLLKQKGTDKP"
     gene            461..1516
                     /locus_tag="PKW73_06660"
     CDS             461..1516
                     /locus_tag="PKW73_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNA72999.1"
                     /translation="MSKNSEFWKPAYLVFWLGLAIGSFFILKELIMMVICLVAAITLA
                     AALSPLARYLEARKVPRLITVISVYLFVAALYFIVGANLTPVIKEQAVALVSTVRDKL
                     QPWIVENIHELAPSILPDTAHGPSLSDDMKTQLMGKAGGIAAGALKLTGNVMTFVVNI
                     LFVLFLTSYFVVESKSLLESLLSWLPPKPRKVVRELLPGLEGRLGGYVRGQLLVSLAV
                     GTIIGIGLFLVGIKEALVLGVVAGFMNLVPFVGSLATAIFAILIGFNQSLTTGCLVIL
                     VFACEQWLESNFIVPQLLGKQVDMHPLVVLFSVLIGGSLLGLSGALIAVPLATASLYL
                     AEELYLKKMEPENSPTA"
     gene            complement(1479..2606)
                     /locus_tag="PKW73_06665"
     CDS             complement(1479..2606)
                     /locus_tag="PKW73_06665"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNA73000.1"
                     /translation="MDHWLLASYRQFLRPWLFLMDAEDAHNLGHKLLSAGAGVMESAA
                     VYLGVENEVGTAALPSSWSNLSLAGRPLHNPLGLAAGFDKNGKLLDGLASLGFGFVEL
                     GSVTALATSGNPRPRLFRLPADQALINRLGLNGDGAVNVASRLKHYQGQGFPLPVALN
                     IAKSNKPWVVQEKAAEDVAFTFKNLRHLPFTYVVINTSCPNTHEGASFAASELTMILK
                     AVQGLNHEAARPLPIFLKLSPDSPESFLDILMPLARSFEVRGFITGNTTVSRSGLRTD
                     AERLATIGNGGLSGAPLLTGTQTMVKEIVLRKEKEQEVIACGGISQADDVETMLKEGA
                     KACQLYSALVYQGPFLVLALLQELKMRNQLRQSANSPVPFS"
     gene            2725..>3231
                     /locus_tag="PKW73_06670"
     CDS             2725..>3231
                     /locus_tag="PKW73_06670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA73001.1"
                     /translation="MSDKPESGRNDPSGKEASFDFRPAKDDETIPLDSAFNRQNPGDG
                     SLIGQVVAGHYEILEVIGEGGMSVVYRARHQHLNRQVAIKFLHGHLVNSRNNLERFKQ
                     EARSVSQLEHRGVVRVYDFGLFQGSRPYIVMDLLKGRSLSQLIRDTVAETGAALPPAR
                     ALPIFIQIA"
BASE COUNT          752 a          889 c          860 g          730 t
ORIGIN      
        1 atttggccgg acagcgcgct ttcgcggagt ttgcggcggc ggccaaggac gagggttggc
       61 tctcggcggg cagttggttc taccggttgg ccgccctcgg cgaataccaa aacaagctgg
      121 ccctgaacgt gccggagaaa accgcttttc gcctgccgct gccgcttgct ctaccctccc
      181 tctccgatca agacgaaagc tgcttgcgga ttgaaatggt caaaacaacc ggtgccggag
      241 acggcgatgt gaacttgctt attctcaaca aagaggggca agtagtggag acccttgccc
      301 tggcaggtag taacggggaa aagacctgcc ggctttcagg tctcacctgt ctggcccgcc
      361 cggatgtagc agacggcggc aaggcggcat tatactttga gccgggcaat tacctgctca
      421 aaggcatcta tcttctgaaa cagaaaggca cagacaagcc atgagcaaaa acagcgaatt
      481 ttggaagccc gcctacctgg tcttctggct tggtctggcc ataggatcat ttttcattct
      541 caaagaactg attatgatgg ttatctgcct ggttgccgct attaccctgg cagcagcact
      601 ttctcccctg gcacgctatc tggaagcccg gaaggttccg agacttatca ccgtcatttc
      661 cgtttatctg tttgtagccg cactgtattt catcgtcggc gccaatttaa cccccgtgat
      721 caaggaacag gccgtggccc tggtctccac ggtgcgggac aagttacaac cctggatcgt
      781 agaaaacata cacgaactgg ctcccagcat cctgcccgat actgctcatg gaccgtcttt
      841 atcagatgac atgaagactc agctcatggg taaagccggt ggaattgcag ccggagctct
      901 caagttgacc ggcaatgtca tgacctttgt ggtaaacatc ctctttgtgc tcttcttgac
      961 cagctacttt gttgtagagt cgaaaagcct gctggaaagc ttgctttcct ggctgcctcc
     1021 caagccacgg aaagtagtaa gagaacttct tcccggtctg gaagggcgcc tgggcggtta
     1081 tgtcagagga cagcttctgg tcagcctggc cgtgggcacc ataattggta ttggtctttt
     1141 cctggttggc atcaaagaag ccctggtact tggagttgtg gccggcttca tgaacctggt
     1201 gccctttgtc ggctcactgg ccacggccat tttcgccatc ttgattggtt tcaatcagtc
     1261 cctcactacc ggttgtctgg tgatcctagt ttttgcctgt gagcaatggc tggaatccaa
     1321 tttcatcgtg ccgcaactac tcgggaagca agtggacatg catccgctag tggtactatt
     1381 ctcggtgctg atcggcggca gcttactggg actctccggc gccctgattg cggtaccact
     1441 ggccacggca tccctctatc tggcggaaga actttatctc aagaaaatgg aaccggagaa
     1501 ttcgccgact gcctgagctg gtttctcatt ttcagctctt gcaaaagagc aagcaccaga
     1561 aacggacctt gatagacaag ggcgctatat aactgacagg ccttagcgcc ctctttcagc
     1621 atggtctcca catcatcggc ctgactgatg ccgccgcaag caattacttc ctgctccttt
     1681 tccttgcgca gaacaatttc tttcaccatg gtttgcgtgc ctgtcaaaag aggcgcgccc
     1741 gataagccgc cattgccaat ggtggcaagc cgctcagcat cagtgcgcaa gccgctgcga
     1801 ctgactgtgg tattaccggt gatgaatcct ctcacctcaa aactcctggc caggggcata
     1861 agaatatcaa gaaaactttc aggactatca ggggacaatt ttagaaaaat aggcaggggt
     1921 cttgctgctt catgatttaa gccctgaacg gctttgagaa tcatagtcag ttcgctggcg
     1981 gcgaaggagg ctccttcgtg ggtatttgga caactggtgt tgatcaccac ataagtgaaa
     2041 ggcagatgcc tgagattctt gaaggtaaaa gcaacatcct cggcggcctt ctcttgcacc
     2101 acccagggct tgttactctt ggcaatatta agggcaaccg gcaggggaaa gccctggccc
     2161 tggtaatgct taaggcgcga agccacattg acggcgccgt cgccattcaa accaaggcga
     2221 ttgatgagag cctgatcggc cggcaagcgg aaaaggcggg gacgaggatt accacttgtg
     2281 gcaagagccg taaccgagcc caattccaca aaaccgaagc ccagagaagc aagcccatcg
     2341 agcaacttgc cgttcttatc gaagccggcc gccagaccta gggggttgtg caatgggcgc
     2401 ccggccagag aaagatttga ccaggaggaa ggtaaggcag cggtacctac ctcgttttcc
     2461 actcccaggt aaacagcggc ggactccata acacccgccc cggctgacaa gagcttgtgc
     2521 cccaaattat gagcgtcttc cgcatccatg agaaagagcc agggacgaag aaattgtctg
     2581 tagcttgcaa gtaaccagtg atccatagtc tttacacaat cattaaatct gccgacagtg
     2641 tttcagtatg ccacgcggat gagcaaattt gtggtgcaat cagggcaaag tgaatataac
     2701 ccctgtgaaa ggccgatgcc tgaaatgtcc gataagccag aaagcggacg aaacgacccc
     2761 tccggtaagg aagcgtcctt cgattttcgc ccggctaaag acgatgaaac cattcccctg
     2821 gattctgcct tcaacagaca gaaccccggc gacgggtcgc tcatagggca agttgtagcc
     2881 ggtcattacg agattctgga ggtcataggc gagggcggca tgtcggtggt ctaccgggcc
     2941 cgccaccagc acttgaaccg gcaggtagcc atcaaattcc tgcacgggca cctggtaaac
     3001 agccgcaaca atctggagcg cttcaaacaa gaagcccgct ccgtcagcca gcttgaacac
     3061 cgtggcgtgg tgcgtgttta cgactttgga ttgttccagg gcagtcgccc ctatattgtc
     3121 atggatctgc tcaaggggcg ctccctcagc cagttgatca gggacaccgt tgccgaaacc
     3181 ggtgccgccc tccccccagc cagagccctg cccattttta tacagattgc c
//
LOCUS       DAOQNZ010000301         3226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15016_length_3226_cov_3.039420, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000301 DAOQNZ010000000
VERSION     DAOQNZ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15016_length_3226_cov_3.039420"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(573..1604)
                     /locus_tag="PKW73_06675"
     CDS             complement(573..1604)
                     /locus_tag="PKW73_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73002.1"
                     /translation="MNYLKFSSNQELRAYIEGLAASNPTEFCMKESPAVTELPPLPAS
                     ITHLEIEDCPALWLLPKLPKSLSFLSLYKCGSLTELPRLPASLVELCIRKCDGIRYLR
                     YLPTALRILRIGCARMVQSPRLPASLQVLELDGCDWLSAVPKLPAGLLALEVRACPNL
                     SRIPALPESLELLSIIACSQVIGLPALPAGVRRFELRQCAQVRSLPEWPAALRSLDLF
                     ACPALVDLSEFPEGLSELTLLDCPAVAVLPALPSSLKSCILRACRAPKLFSTLPQSLT
                     SLLVIDSAIAELPALPAALKSMLVEKCPNLSRLPKLPDSLQSLIIRDCAGLYPNQKLI
                     LGSLEIRLE"
     gene            complement(2204..3088)
                     /locus_tag="PKW73_06680"
     CDS             complement(2204..3088)
                     /locus_tag="PKW73_06680"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HNA73003.1"
                     /translation="MSTNSETTTVTPAAGAEAPAIATPDADKPKGKTLDEQLKELQIA
                     EARLRVEKARTDKTTAAQEAGLKLDLTRTQLRSAQLDLEKKEREAAREKASADENLTY
                     TFYDGVTDESIKPSLQKLGDWNRRFPGKPITVIVNSPGGSVLAGLALYDYLQKLRADG
                     HHITVICFGMAASMGGILLQAGDKRMLGANAKVLIHAVSGGTIGTVHAMEDRVAFSRN
                     LWEGLKKILATRSVLNEAEIEERAHRKDWWLDADEAIALGFADEIVPAPAFAGAKPKA
                     QVKVQSGKSRRRRGRSGK"
BASE COUNT          843 a          881 c          870 g          632 t
ORIGIN      
        1 agccccacgg tcggaaaacg aaaggcaggt caggttggtc tccagacaaa gcgcggaccg
       61 tcaccggcag cttagaactg cacgagaacg atagagacaa tgaaggagaa cgtttttgaa
      121 ttgcttttac aaagcttgga aaattaggga tatacaaatg ctcgtagcaa agcccggtta
      181 tcaagagcaa taagataatg aacagatctc tgcttattta gcagcactgt aatagcgctc
      241 atgaaattgt ctcagaccaa aatgcgcaac cggcccgcac aacaacagag tttcgccaca
      301 cttgccgacc atttgcggtc atcaaaacgg cgtctgagat ccacatagaa gcgaactcgc
      361 cgaccggtct actacttcct ggcaaaaaaa ccacaggcag ctgttgggga ctcattttca
      421 acatcctgtg gtagccctgc cctgatcaaa tagcagaagc gaggccgaga agaattgaca
      481 ccggtagtca ggactggagt ttccaacctg gccaccggcg tcacactaat tactggtcca
      541 cgctatgagc aagtcggccg ccatccaaca cgctattcaa ggcgaatttc aagacttcct
      601 aaaatcagtt tctggttcgg gtagagcccg gcacagtctc tgatgatcag gctttgcagt
      661 gaatccggca gtttcggcaa gcgcgataga ttgggacatt tttctaccag catcgactta
      721 agagcggctg gcagtgcggg caattctgct atggcactgt ctatcaccag caaggaagta
      781 agagactgtg gcagggtcga aaacaatttc ggcgcccggc aagctcgtaa aatacaagac
      841 ttgagagaag acggcagtgc gggaagtacg gccacggcag gacagtcaag aagagttaac
      901 tcggacaaac cctcaggaaa ttcagacaaa tccacaagag ccgggcaagc aaaaagatcg
      961 agtgaccgca atgccgcagg ccattcgggc aaagaccgca cttgagcgca ttgcctcagc
     1021 tcaaagcgcc gcactccggc tggcaaggca ggcaagccaa tgacttgaga acatgcaatt
     1081 atcgatagaa gctcaagaga ctcaggtagc gcaggtattc tgctcaagtt cgggcaagcc
     1141 cttacctcaa gcgcaagcaa gccggcaggc agttttggga cggcagaaag ccaatcacag
     1201 ccatctaact ccaatacctg aagagacgcc ggcaacctcg gcgactgcac catcctggca
     1261 caaccaatgc gcaatatcct gagagcggtc ggcaagtatc taagatacct gattccatcg
     1321 cactttctaa tacaaagctc caccagcgat gccggtagtc tcggcagttc ggtgagactg
     1381 ccgcatttgt acaaactgag aaacgaaagg gacttaggca attttggcag aagccagagc
     1441 gccggacagt cctcaatttc aaggtgtgtg atggaagccg gcaggggagg caactccgtt
     1501 acagccgggc tctccttcat acaaaactct gtgggattgg aagcggcaag gccttcgatg
     1561 taagctctta actcttgatt agaactaaac ttcaagtaat tcatatacgc accatagtaa
     1621 aaacgagaat taaattgtct ggttgcaacc gttccgcagg gctgacaacc ctccgaagcc
     1681 gcccggccgg tgaaccatct ggaacctttt ttgactgacg atcttgttgc ctgaaagaga
     1741 ccttgcagga agaatgaggc gcgcacaact ccagaaagct ggttcttgct caaagagggt
     1801 tgatttctag cgtctgcttg gctctagttg caaattctac tgactgactc cggcgaaagt
     1861 aaaaacaagg tttcacccag tacactatgg tgcaagagaa gcagaaacaa cgcatttggt
     1921 cgaagttttg cggcaaacac caccattcca aaagaaatca gttcggccga catgtcaaag
     1981 tctcccgacc gagcggagat tcggcagatc gagcgccacg gtaaagtcac gataaaacaa
     2041 acggaagatg aaaaaaaaca gcaggattat gccactgcca gaggtgccct tttcagactc
     2101 cactatattt actaataatt ccgaccaaaa agggggtgcg cctgtgaaag cgcacccccc
     2161 cggaacaaag ttcaaggaga gctcagttag agccctcccc gaattactta ccgctgcgtc
     2221 cgcgacggcg acggctcttg ccggactgaa ccttgacctg agccttcggc ttggcaccgg
     2281 caaaagcggg tgccggcacg atctcgtcgg caaagcccag ggcaatagcc tcgtcggcat
     2341 cgagccacca gtccttacgg tgagcgcgtt cctcgatctc ggcctcgttc aagaccgagc
     2401 gggtggccag aatcttcttc agaccctccc agaggttacg cgagaaggcc acccggtctt
     2461 ccatggcgtg aacggtaccg atggtaccgc ccgacacggc atgaatgagg accttggcgt
     2521 tggcaccgag catgcgcttg tcgccggcct gcaggagaat gccgcccatg gaggcagcca
     2581 tgccgaagca gatcaccgtg atgtggtgac cgtcagcgcg cagcttctgc aggtagtcgt
     2641 agagcgccag accggccaac accgacccgc cgggcgagtt gacgatgacg gtgatgggct
     2701 tgccggggaa gcgccgattc cagtcaccaa gcttctgcaa ggaaggcttg atgctctcgt
     2761 cggtgacgcc gtcatagaag gtgtaagtga ggttctcgtc ggccgaagcc ttctcccgag
     2821 cggcctcacg ctccttcttt tccagatcga gctgggcgct gcgcaactgc gtgcgagtca
     2881 aatccagctt caatccggct tcctgagcgg cggtggtctt gtcggtgcgt gccttctcga
     2941 cacgcaaacg agcctcggcg atctgcaact ccttgagctg ctcgtccagg gtcttaccct
     3001 tgggcttgtc agcatcggga gtggcgatag cgggcgcctc agcgcctgcc gccggggtga
     3061 cagtggtagt ttcagagtta gtactcatga tttgtctctc gattctgcac cacctcagcg
     3121 ggcggtgcgg ttaaactccg ataaattgca ggatgaaagg gcaagcacca aaagcggtgc
     3181 tcacccccgg gaaagagcgg caaccgcgcc gacaccgctc ggagga
//
LOCUS       DAOQNZ010000302         3226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15018_length_3226_cov_2.877641, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000302 DAOQNZ010000000
VERSION     DAOQNZ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15018_length_3226_cov_2.877641"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKW73_06685"
     CDS             complement(<1..255)
                     /locus_tag="PKW73_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73004.1"
                     /translation="MAVAFDTNAIKFSSGPYKMRPGLVPLCVALAMGYVYVGIWFTVA
                     CFVFSENLIWSILIMCSSLGFAVFLSIMTFCLYRDSQRRYE"
     gene            complement(316..1851)
                     /locus_tag="PKW73_06690"
     CDS             complement(316..1851)
                     /locus_tag="PKW73_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNA73005.1"
                     /translation="MELFKNINLSLQALVLITALAAYHVKEARLKTIAWRCLLLACTA
                     LVIVVMGHTALNEARQDKAAANMDVGQVEFLMRLHNAFKNMSSIDLPLPAGSPLDSES
                     GKVLSSARKSMAAAVEKEPQSFSIRMKEAILLGELNAPVKASLESLAKNKDPRAAQAI
                     KVLEAFYVNKRLPKEELNATLAFLDDAFPRGWFRDVSHIEALKLAGNKKAADEALVRY
                     QDSSTQLVWRLLIIMVVAAFAAVTGVIVLLTQLFLLPRSLSKDASHEEIKAPRDWGYD
                     KVYGVFVGWLSLECMISPLLVKVAQPLKALGLGPNNVAIITIVIYLLTNLPALLFAWF
                     FVVRPSGENFFAATKVRLKTPRRGWFGLVMAGVLTWFASVPVVIVGSFLAKGIFNSQG
                     SSNPILAVVMDAARNGDALTVVLFVIALGVLPGICEELLFRGFLYTFLRRRMGALLSM
                     VISATVFAGVHLDKGAFLQLFILGFAFAFVFERTRSLIPCMVAHCMWNSGTFFLINAI
                     FGS"
     gene            complement(1946..2323)
                     /locus_tag="PKW73_06695"
     CDS             complement(1946..2323)
                     /locus_tag="PKW73_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73006.1"
                     /translation="MQHPSGLCLPVVGIENMQFWNIAIFEFFFVLFVMIGLGFITVPR
                     FLMRRRLMKGFPWGYYADQVPQGDPSLFPGNDKDFLHDTGTAFGGLEGFGEYGEFSGF
                     GQLGEYGGGFFSGGGDGGGGGVE"
     gene            complement(2471..2683)
                     /locus_tag="PKW73_06700"
     CDS             complement(2471..2683)
                     /locus_tag="PKW73_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNA73007.1"
                     /translation="MPKKVLVALPPGLLEQIDFVASVEHRTRSDLIREALRRYIDGFK
                     RNQGPRMQVSQVGNQKMAILSDSELA"
BASE COUNT          936 a          870 c          790 g          630 t
ORIGIN      
        1 ctcataacgc ctctggctgt cccgatagag gcaaaaggtc atgatgctca agaatacggc
       61 aaaccccagc gaggagcaca taatgagaat ggaccagatg aggttctcgg aaaatacaaa
      121 acaggccacg gtgaaccaga ttcccacata gacatagccc atggccaggg caacgcacag
      181 gggaacgagc cccggtctca ttttgtaagg accggaacta aattttatgg cattggtatc
      241 aaaggctaca gccataagcc ctcagcaagc caccttaata gttatgccca gagggagggc
      301 aaaactaaag caaggctagc tgccaaaaat ggcgttaatc aggaagaaag tgccgctatt
      361 ccacatgcaa tgggccacca tgcagggaat caaggagcgc gtccgttcaa aaacaaaggc
      421 aaaggcgaag cctaaaatga aaagctgcaa gaaagcacct ttgtccaggt gcactccggc
      481 aaagaccgta gccgaaatca ccatggaaag cagggcaccc atacgccggc gcaagaacgt
      541 ataaaggaag ccccgaaaga gcagttcttc gcaaataccg ggcagtaccc cgagggcgat
      601 gacaaagagc actaccgtaa gggcatcgcc gttacgagca gcatccatga ccaccgccag
      661 aatcggattg gaagaaccct gggagttgaa gattcccttg gccagaaacg aacctacaat
      721 caccacaggc acgctggcaa accaggttaa gacaccggcc ataacgagcc cgaaccagcc
      781 ccgcctcggc gtcttcagcc ttactttcgt ggctgcgaaa aaattctcgc cgcttggcct
      841 gacgacgaaa aaccaggcaa acaaaagggc cggcaaattg gtcaaaaggt aaataacgat
      901 agtaatgata gctacattgt taggtcccag gccaagagcc ttaagcggct gggctacttt
      961 caccagcaag ggtgagatca tgcactccag ggacaaccag cccacaaaca caccgtaaac
     1021 tttgtcatac ccccaatctc tgggagcctt gatttcctca tgggaagcat ctttggagag
     1081 ggaacgaggc aaaagaaaga gttgggtcaa taggacaatt acaccggtga cggcggcaaa
     1141 ggccgctacc accataataa tgagcaaacg ccagaccagt tgagtagagc tgtcctggta
     1201 acgaaccagc gcctcatcgg cggccttctt attgcctgcc agtttcaggg cctcgatatg
     1261 gctgacgtca cgaaaccagc cccggggaaa agcatcatcc agaaaagcca gggtggcgtt
     1321 gagttcctct ttcggcaagc gtttgtttac gtagaaagcc tccagcactt tgatggcttg
     1381 ggccgcccgg gggtctttgt tcttagccag actttccaga ctggctttca ccggggcatt
     1441 aagttccccc agaagaatgg cttctttcat gcgaattgaa aaagattggg gctctttctc
     1501 cacagccgcc gccatggact tgcgggcgga ggagagaacc ttaccggact cgctatccag
     1561 ggggctcccg gccggtagag gcaggtcgat ggaggacata ttcttgaaag cgttgtgaag
     1621 ccgcatcagg aactccacct gaccgacatc catgttggcc gcggccttat cctgcctggc
     1681 ctcgtttaag gccgtatgac ccatgaccac aatgacaagg gctgtgcagg ccagtaacag
     1741 gcaacgccag gcaattgtct ttaagcgtgc ttccttgacg tggtaggcag ccagggccgt
     1801 aatgagcacc agggcctgca aggacaggtt tatatttttg aaaagctcca aagcaaacta
     1861 cccgagcccg atgccgtagc gaacaaagct aggatagcaa atcgtaaaag gttttgttat
     1921 ggaagcactc gcttgagggt tgcccctact ctacgccccc accgccgccg tcgccgccgc
     1981 cggagaagaa gccgccgccg tattcaccca actgaccgaa gccgctgaat tcgccgtact
     2041 ctccaaaacc ttccagacca ccaaaggctg taccggtgtc atggaggaag tctttatcat
     2101 tgcccgggaa gaggctgggg tcgccttgcg gaacctggtc ggcatagtaa ccccagggaa
     2161 agcccttcat cagtcgccgc cgcatcaaga agcggggtac cgtgataaaa cccagcccga
     2221 tcatcacgaa caggacgaaa aagaactcaa agatggctat attccagaac tgcatattct
     2281 cgattcccac caccggcagg catagccccg aggggtgctg cattgcagat tgccggccca
     2341 ttttgctgga accaacaatt tgtcacaatg gatattagaa ggtttcagac tccctgacaa
     2401 gggcaaaatt gcccagccag tggggaaact actaactttt ccggaacaat tatctaataa
     2461 ggcttgaaga ctaggccagt tcgctgtcgg acaggatggc catcttttga tttccgacct
     2521 ggctgacttg cattctcggt ccctgatttc tcttgaaacc atctatataa cggcgcagag
     2581 cctctcttat caggtcggag cgagtgcggt gctccaccga ggccacaaaa tcgatttgct
     2641 caagcaaacc cgggggcaga gcaactagta ctttcttagg catgcacgtt cctttttgta
     2701 aaatccaaaa aagtatcaac caaaatttac gaaatcagcc aagttacaac aggccatggc
     2761 caggtaaact agacacaaca agatggacgg acgcaagctc cccgtcttta ttgggcgtga
     2821 ccgccgattt ccactagtaa atttacactc tacaggcacc tttttaaaga ggcgcaaagc
     2881 ccataaacag tagagaataa acaaactaat ctaacggact tctcctgtca cccaaaacaa
     2941 ctcccggaca acatgcatca aatgcggtag caaagccatt ttcagcacct tgtccgacaa
     3001 cgaaaacaaa atccgcgcag tagttattca ggaaaaattt taagcaacca ctacaaaagc
     3061 tagctttgat gcggatattc agagaacata cactagtcca aatatatgtc caacaataat
     3121 ccaaaagacc tatcatcgaa cgacaagcgc ttgacgagta atgtcaaagc tgccggttgt
     3181 gcggcaaaaa tgagttcagc cgaactcaaa caagtgcttg ccggac
//
LOCUS       DAOQNZ010000303         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15036_length_3224_cov_3.852950, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000303 DAOQNZ010000000
VERSION     DAOQNZ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15036_length_3224_cov_3.852950"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2341)
                     /locus_tag="PKW73_06705"
     CDS             complement(<1..2341)
                     /locus_tag="PKW73_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNA73008.1"
                     /translation="MKAVIMAGGSGTRLRPLTCNLPKPMVPVAGKPMAEHIVNLLKEH
                     GFDDIVFTLHYLPDAIRDHFGDGSEFGVKISYSVEEGKPLGTAGCVKAIQDHLDSTFI
                     VISGDSLTDMDLGKALAFHKEKKSKATLILKRVENPLDYGVVITDNEGRIQRFVEKPG
                     ASEIFSDTVNTGTYILEPEMMLYVVMGREQDFSNDLFPLLLLRNEPMFGYVADGYWCD
                     IGNLAVYRQAHRDVLDGLVNIKMDLPQIEPGIFVGHGTQIDSSVTLEAPVMIGKNCRI
                     GRETVISQYTTIGDNVVIQEKASLKQPVIWSNSYIGNNAQLRACVVCNNATIHNSAEL
                     LEGAIIGNNSVVGQEARISPDVRIWPDKNIDSGAKVLTSVIWGTRAPRTLFGAHGVRG
                     LANVDITPEFAVNLAAAYGATLKGGPVLVSRDYWKVSQMISRAMVSGLVSVGIEVQNL
                     ESMSLPISRYYVKTQRAAGLVHVRVSQREIDKVTIEFFDSQGVAITKSMERKIETTFF
                     KEDFPRCAPSDVGTISFPSRVREYYADEFLNHVKGQVFEEDKVPFCIVPGSNYTRKTK
                     VGGLSTHAPKVVIDYAMAETGVLLPDLLGQLGIETVVLNSSIRNSPPRQEERITMRKQ
                     LADVVKALGADLGVQIGRNGEQMTLVDETGQIIRGELLLATVADIMLRDKPGRSIVVP
                     VNASSVVERIAARNGCKVVRCKASETEIGSTTARLPEAVLGGSANGCFIFPEFQNGYD
                     AMFAVGQVLEHLTYQGRTLQQAINELPPLYYQVDSVHCPW"
     gene            complement(2350..>3224)
                     /locus_tag="PKW73_06710"
     CDS             complement(2350..>3224)
                     /locus_tag="PKW73_06710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA73009.1"
                     /translation="TEAGRMHGIHNEMQRYIHQMEWRLTFEAWEVIVNSHAMHGELQA
                     LFGLPANKIAVVPNGTDPSVFDFHYDARQTRSRYVSEHQQIVLYVGRMVREKGVHVLL
                     ESVPRVISERPGTQFLFVGTGYYLDDLRRQAQYMGISHCTHFLGYVGDEELKHLYKSV
                     DAVCIPSLYEPFGIVALEGMAAQVPVVTSDVGGLKDFVEHMETGVTTYAGDAGSLAWG
                     LLQVLRNPELSNYLRETAYDKVQHIYNWKVIARRTREVYQKVLRESEIVASGLNNVVE
                     SEAVKTVAAKGKGR"
BASE COUNT          752 a          898 c          859 g          715 t
ORIGIN      
        1 cccaggggca atgaaccgag tctacctggt agtagagcgg cggcagttcg tttatggctt
       61 gctgcaaggt acgcccctga taagtgaggt gctccaatac ctgtcctacg gcgaacatgg
      121 catcgtagcc gttctggaac tcggggaaga taaagcagcc gttggcgctt ccacccagaa
      181 ccgcctccgg cagacgagcc gtggtgctac cgatctcggt ctcggaagct ttgcagcgaa
      241 ccaccttgca gccgttccgg gccgcaatgc gctccactac ggagctggcg ttcaccggca
      301 ccacgatgga acgtcccggt ttgtctctca acatgatgtc ggcaacggta gccagaagca
      361 gctcgcctcg aataatctgg ccggtttcat ccaccagggt catctgttcg ccgtttcgtc
      421 cgatctgtac acccagatcg gcgcccagag ccttaaccac atcagctaat tgcttgcgca
      481 tggtgatgcg ttcttcctgc cggggcggcg aattacgaat ggatgagttg agcaccacgg
      541 tctcgatgcc caattgcccg agcagatcgg gcaaaagcac accggtctca gccatggcat
      601 agtcgatcac taccttggga gcatgggtgg acagtcctcc caccttggtc ttacgggtgt
      661 aattggagcc tggcacgata cagaagggca ctttgtcctc ttcgaaaacc tgccccttca
      721 catggttgag aaactcatcg gcataatatt cccgcacacg gctggggaag gaaattgtgc
      781 ccacgtctga aggggcgcaa cggggaaagt cttccttgaa gaaggtggtt tcgatcttgc
      841 gctccatcga tttggtgata gccacaccct ggctgtcgaa gaattcaatg gtgaccttgt
      901 ctatctctcg ctgtgatacc cgcacatgca caagaccggc cgctcgctga gtcttcacgt
      961 aataacggga aataggcaga gacatagact ccaggttctg tacctcgatg cccaccgaga
     1021 ccaagccgga gaccatggcc cggctgatca tctgagaaac cttccagtaa tcacggctga
     1081 ccagcaccgg accacctttg agagtggcac cataggcggc ggcaagattg acggcaaatt
     1141 caggtgtgat atccacatta gccagaccgc gcacgccatg ggcaccaaat aaggtgcggg
     1201 gtgcacgggt gccccaaatc accgaggtga gcactttcgc gccgctgtcg atgttcttgt
     1261 caggccaaat gcgcacatcg gggctgatgc gagcttcctg tccgaccacc gaattgttgc
     1321 cgatgatcgc cccttccaaa agttcggcgg aattatggat ggtggcattg ttgcaaacca
     1381 cacaagctct caactgggcg ttattaccga tataagagtt ggaccagatg accggctgct
     1441 tgagagaggc tttttcttga atgaccacat tatcgccgat ggtcgtatac tgcgaaatca
     1501 cagtctctct gccgatgcga cagttcttgc cgatcatgac gggcgcttcc agagtgacgg
     1561 aagaatcaat ctgggtaccg tgaccaacaa aaatgccggg ctcgatctgg ggcagatcca
     1621 tcttgatgtt gaccagccca tcgagcacat cccggtgcgc ctgtctgtag acggccaggt
     1681 taccgatgtc gcaccagtag ccgtcggcca cataaccgaa catgggctca ttgcgcagaa
     1741 gcaagagcgg gaaaagatca ttggagaagt cttgctcccg gcccatgacc acataaagca
     1801 tcatttccgg ctcgagaata taggtaccgg tattgacggt gtccgagaaa atctcggagg
     1861 cgcccggttt ctccacgaag cgctgaatgc gaccttcgtt gtcggtaata accacaccat
     1921 agtcgagggg gttttccacc cgcttcaaaa ttagtgtggc cttggatttt ttctctttgt
     1981 gaaaagctag agccttgccg agatccatat cggtgagact gtcaccggaa atgacgataa
     2041 aagttgaatc aaggtgatct tgaatggctt tgacacaacc ggcggtgccc agtggtttgc
     2101 cttcttccac ggaataactt attttgactc cgaattccga gccgtcgccg aagtgatcgc
     2161 ggatggcatc aggcagatag tgcagggtga aaacgatgtc gtcaaagccg tgctctttca
     2221 agagattgac gatgtgttca gccatgggtt taccggccac aggcaccatc ggcttgggca
     2281 ggttacaggt aagcggtctg agcctcgtgc cggaaccacc ggccatgatc acagctttca
     2341 tgctcttact tacctcccct tgcccttggc ggcaactgtc ttgacggctt cgctctccac
     2401 tacattgttc aggcctgaag ctactatttc agactctcgc aatacctttt gatagacttc
     2461 ccgggtgcgt ctggcaatca ctttccagtt gtagatgtgc tgcaccttgt cgtaggcagt
     2521 ctcacgcagg taattggaaa gctcgggatt acgcaagacc tgaagcaagc cccaggccag
     2581 agagccggca tcaccggcat aggtggtgac gccggtttcc atatgttcca caaagtcttt
     2641 cagaccaccc acgtcggatg tgaccaccgg cacctgggcc gccattcctt ccagagccac
     2701 aataccgaaa ggctcataaa gactggggat acatactgca tccaccgact tatagagatg
     2761 cttgagttcc tcatcaccca catatccaag gaaatgagtg cagtgcgaaa tgcccatata
     2821 ctgcgcctgt cgccgcaagt catccagata gtagccggtg ccgacaaaga ggaactgcgt
     2881 acccggtctt tccgaaatca cacgcggcac tgattcgagc agcacatgca cgcctttctc
     2941 acgcaccatg cggcccacat aaagaacgat ctgctgatgc tcggagacat agcgactgcg
     3001 ggtctgacgg gcgtcataat ggaaatcgaa taccgaagga tcggtgccgt ttggaaccac
     3061 ggcaatcttg ttggcgggca aaccgaagag cgcttgcaat tcaccgtgca tggcatggga
     3121 attgacgatt acttcccagg cttcaaaagt aagacgccat tccatctggt gaatatagcg
     3181 ctgcatctcg ttatgaatgc cgtgcatacg tccggcctcg gtgg
//
LOCUS       DAOQNZ010000304         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15098_length_3216_cov_4.253717, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000304 DAOQNZ010000000
VERSION     DAOQNZ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15098_length_3216_cov_4.253717"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PKW73_06715"
     CDS             <1..905
                     /locus_tag="PKW73_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73010.1"
                     /translation="TNHLDRQLNIRGAIPTMQTTDDYLWFDNRTDEKLVRERIEEQAK
                     TIKRLTISNQAFLQNGLFHGLLLPELQELVIPSLFYFDNSKGWQFLHGLPKLEILVAE
                     YTEAKDSILEGLSTAPWWESLTQLELKLANLKETTKWSTLWGEKNFQLKILCLFYLDP
                     EKAEQVLTGTMDKLEYLIMGTDLGRDFLLKLAKKALPALADLDLSHSSMQLEETLKFL
                     REPRPGLPKLERVRKQIASEERVEYYDWNGAAVDWGYRELSDSEIQKKYFKKTGLKIL
                     PANEQLASRTAMMSSALRKLERPK"
     gene            complement(940..1686)
                     /locus_tag="PKW73_06720"
     CDS             complement(940..1686)
                     /locus_tag="PKW73_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73011.1"
                     /translation="MNNRTKPGVACSVGKLILNSGIALLGFLISAPTAIAGDFEKGTG
                     LYNARDYAGAAQAFQRVVKGSPTNWQAHYYLGHVNMALGNFAAARKAYEACLKYTTDG
                     SVKGQCLTSIERCKAAVQAGASSTVPPSKAVSPSRPQSGTSAPGKAASAADSSNPVSP
                     TAVVNDANVQQMRAHINQEAQAAVARIRAEAREQIKHEKEKSNQEFRYKDGSTGYDIS
                     DEREQEILRDAEAECAKVMKDAEHRLKFLK"
     gene            complement(1834..2598)
                     /locus_tag="PKW73_06725"
     CDS             complement(1834..2598)
                     /locus_tag="PKW73_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73012.1"
                     /translation="MRRFCRYLKLLTVALISGQCIAPALAGDFDEGVKLYQSSNFRGA
                     LPYFEKAARDFPTSWQVRFYLGHTYFALGKMTAARSQYEYVQNSGADAGAKTQAQDAL
                     NKVCKYLGVAAPAPLAVSGAAGAADKPVAGARPAASSGAAGKGKSDAEEDEEEEDEPD
                     QQHHSSGNDDRSKRLQALRESILKVAQSEVAKIKEETKEKLEHEKTQANQYWRFSDGS
                     IRLDISSEREAEINRECTQRCKQVMDEAERRIKNLK"
     gene            2884..>3216
                     /locus_tag="PKW73_06730"
     CDS             2884..>3216
                     /locus_tag="PKW73_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73013.1"
                     /translation="MKQPKKSRNPNRTELVRILALIEENLDEIDREHEAASAYHRSEL
                     VEVSRDNAQAALKEAVQFLALEDFPKAEREATAAWFYTDFARNILDAEFTEHHLGEDI
                     FLDLVPTRE"
BASE COUNT          849 a          884 c          735 g          748 t
ORIGIN      
        1 aaacgaacca tttagaccgc cagctcaaca ttcgcggagc gattccaacc atgcagacca
       61 ccgacgatta cctgtggttc gacaatagaa ccgacgaaaa actcgtcaga gaaagaatcg
      121 aggaacaggc aaagacaatc aagcgcctca ctatttcaaa tcaagccttc ctacaaaatg
      181 gactatttca tggactgctt ctacctgaat tgcaagagct agtaatacca agcctattct
      241 atttcgacaa ttctaaaggt tggcaatttc tgcacggttt accgaaacta gagattctgg
      301 tggcagagta taccgaggcc aaagactcca tcctggaggg gctgtccaca gcgccctggt
      361 gggaaagtct aactcaactg gaattaaagc tcgccaacct gaaagaaacc acaaaatggt
      421 caacgctttg gggcgagaaa aacttccagc taaagattct ctgtctgttc tatcttgacc
      481 ctgaaaaggc ggagcaggtg ctaaccggca ctatggataa gctcgaatat ctcattatgg
      541 gcaccgacct gggccgagac tttctgctca aactggcaaa gaaagcgctt ccggccctgg
      601 cagatctgga tttaagccac tcaagcatgc aactggaaga aacactcaaa tttctgagag
      661 aaccaagacc aggtttacca aagctagagc gggttcgcaa gcaaatagcc agcgaagaac
      721 gcgtcgaata ttacgactgg aatggtgcgg ccgtagactg gggttataga gagctatcag
      781 acagtgaaat ccaaaaaaaa tacttcaaaa agaccggctt aaaaatactc ccggccaatg
      841 aacaactggc atccagaacg gcaatgatgt ccagcgccct gagaaagctg gaacggccca
      901 aataaccagt cagacccctc gagaggctag cggcaacatt tacttgagaa atttgagtcg
      961 gtgctcagcg tctttcatga ctttcgcaca ttcagcttca gcgtccctga ggatctcttg
     1021 ctcacgctca tctgaaatat cgtaaccagt gctgccgtct ttataacgaa actcttgatt
     1081 cgacttctct ttctcgtgct tgatttgctc tctggcttcc gctcgaatac gcgccactgc
     1141 agcttgtgct tcttgattga tgtgcgctcg catctgctgc acattagcat cattaactac
     1201 agccgtcgga ctaaccgggt tgctgctgtc ggcagcggaa gcagcttttc caggagctga
     1261 ggtaccgctc tggggacggg acggcgacac cgcctttgat ggcggtactg tggaggaagc
     1321 accagcttgc acggctgcct tgcagcgctc aatgctagta aggcattgcc ctttcaccga
     1381 tccatccgtg gtgtacttca agcaggcctc atatgctttc cgagcggcag caaaatttcc
     1441 gagagccata tttacatgcc caagataata gtgagcctgc cagttggtag ggctgccctt
     1501 caccactcgc tgaaaagctt gtgcagctcc ggcgtagtcg cgagcgttgt ataaaccggt
     1561 gcccttctca aaatctcccg ctatggccgt cggtgccgat atcagaaagc ccaaaagtgc
     1621 aatcccggaa ttcaatatca acttgcccac actgcaggcc acacctggtt tagttctatt
     1681 attcacgaac actccttctt ctcgaacact cgttgctttc cacgaacgct gtttcatctc
     1741 gaaagcgcgt tcttcaacaa agctccctct tcgcgtatat ggctttctgc tgagaccaca
     1801 acccggtaag actcatcata aaatgaagca ttcctacttt agattcttta tgcgccgctc
     1861 agcctcatcc atcacctgct tgcaacgctg agtacattcc cggtttatct ctgcttcacg
     1921 ctcgctactg atatccaggc gaatactgcc atcggaaaaa cgccagtact ggttagcctg
     1981 ggtcttttca tgctctagct tttccttggt ttcttcctta atcttggcca cttccgactg
     2041 ggcaaccttg agaatactct ccctcagagc ctgcaggcgc ttgcttctgt catcgttgcc
     2101 gctggaatga tgttgttgat ccggctcgtc ctcttcttct tcatcctctt cggcatcgct
     2161 tttacctttg ccggctgcac cagaacttgc ggcgggtctt gcgcctgcaa caggcttgtc
     2221 ggcggcaccg gccgcgccgg aaactgccaa cggagcaggg gcagcaacac caaggtactt
     2281 acaaactttg ttgagagcgt cctgcgcctg tgttttagcc cccgcatcgg caccggaatt
     2341 ctgcacatac tcatactgac tgcgcgcagc cgtcatttta ccaagggcaa aataagtatg
     2401 ccccagatag aaacgaacct gccatgaagt cggaaaatcg cgggcagcct tttcaaagta
     2461 aggcaaggca cccctaaaat tactgctctg atagagcttc acaccttcgt caaagtcacc
     2521 ggctaaggcc ggcgcaatac attgcccaga gataagggca acagtgagca atttcaggta
     2581 tcgacaaaaa cgtcgcatca ggaaatctcc cagtgatgct cgcaaacatt caggaaacca
     2641 tgatacctca gtaaactgcg tttgaccatt ttttacacat ttgacttgcc gagcccgaca
     2701 gctcttaagg aatgaacaaa aaccctccac atacctgctt ttcaggctgc cctgaacatc
     2761 caaataagct atagcaggcc actttcaatc gaaatttcga catgggttct accaaattta
     2821 aggccgaatt cttgctttgg gaagagacgg gcataatatg aaatgtagac cctgaggata
     2881 gcagtgaagc aacccaagaa gagccgaaac cctaaccgca ccgaactcgt gcgcatattg
     2941 gcattgattg aggaaaacct cgatgaaata gacagagagc acgaagcggc ctcggcctat
     3001 catcgcagcg agctggtgga agtctccagg gacaatgccc aggccgcatt gaaagaagcc
     3061 gtgcaatttc ttgccctgga ggacttccca aaagccgaaa gagaagcaac cgccgcctgg
     3121 ttctacacag attttgcccg caacatcctg gacgcggaat tcaccgagca tcacttaggg
     3181 gaagacattt tccttgacct ggtgcccacc cgagag
//
LOCUS       DAOQNZ010000305         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15112_length_3215_cov_2.820570, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000305 DAOQNZ010000000
VERSION     DAOQNZ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15112_length_3215_cov_2.820570"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKW73_06735"
     CDS             complement(<1..456)
                     /locus_tag="PKW73_06735"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73014.1"
                     /translation="MFSREIQFTPISLTPKAGWLESARQSIKLPCAFAMGAVLGFATP
                     GFDQDYLAWFALAPLLVLIRGCQNGFQATLTGFVFGAGYYAIALSFFTGLSPLRWMQI
                     PDILGYQLVFLAWALETCHLALLFAAFALMVYHLPTRPGFLPHFRRPFYP"
     gene            524..1027
                     /gene="ruvC"
                     /locus_tag="PKW73_06740"
     CDS             524..1027
                     /gene="ruvC"
                     /locus_tag="PKW73_06740"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNA73015.1"
                     /translation="MKPEEKTRRILGIDPGTATVGFGVVEVSGINKFAYIASGVISTP
                     KTLPTGARLKMIREDILSIYDEYLPREAAVEALFFFKNAKTLVPVAQARGVILEALSC
                     RNQIPGEYTPMQVKLQISGYGKAEKHEVQWAVARLLNLNEIVKPDDASDALAIAVCHA
                     RMASLPV"
     gene            complement(1028..1708)
                     /locus_tag="PKW73_06745"
     CDS             complement(1028..1708)
                     /locus_tag="PKW73_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73016.1"
                     /translation="MSPEEESNTKSKWKPLQYLAASGLVPLLSALHEPSYLPPLLYTL
                     FVAVYLFHNLKPPEKAEKPVPPLWLCLHFCFLFTACGITACYLSRIISQTALVVMVVY
                     FLTQALCFLFLRSCFPLRLWQAFLLMTISDPLMPLEGDFLAQAKAFDLDTLGRLVNLI
                     MTAGAVAGLPLTVLAEPLKSLPVKSFPPAGKGSSFLRRIMLPALAALSVIVAPKVVAL
                     CTLGELYR"
     gene            complement(1716..2660)
                     /locus_tag="PKW73_06750"
     CDS             complement(1716..2660)
                     /locus_tag="PKW73_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNA73017.1"
                     /translation="MTAPRIVSLIASSTEIIHALGLGHYMVGRSHECDFPASVKSLPV
                     CTAPKFNVDGSSKEIDARVKEILEKSLSVYLVDARKLDELAPTHVLTQAQCEVCAVSL
                     KDVQAAACELIHSRPEIISLSPNCLEDYFLDLEKVGASLQVEAAAQTLIEVSRKRLAE
                     VKEKARRYLCESGSAPLKVAVIEWIEPLMAAGNWMPELVEMAGGINLFGEAGRHSPWM
                     DFASLQSREPDVIIVTPCGFDNERTLKEMPALCNIPGFHDLQAVKNERLYIADGNQYF
                     NRPGPRLMDSLEILAEIFYPQAFPARYQESWLNYSRVS"
     gene            complement(2657..>3215)
                     /locus_tag="PKW73_06755"
     CDS             complement(2657..>3215)
                     /locus_tag="PKW73_06755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNA73018.1"
                     /translation="LERFFLQDISGHCGQNALAEYIASLYDLSLSFADLEWIVAEAGL
                     PVLVKGILRGDDARKAVAAGAAGIIVSNHGGRQLDTAVSSIEALEEICRAVPDTTVLM
                     DGGIRRGTDILKALALGARAVLVGRPVLWGLAWQGESGVRSVLSTLQEELLLSMQLAG
                     VASLKDVERDLVKSPFFAGGQGERL"
BASE COUNT          720 a          857 c          941 g          697 t
ORIGIN      
        1 aggatagaac ggtcgcctga agtgaggcag aaagccgggt ctggtcggca agtgatagac
       61 catgagggcg aaggcggcga agagcaaggc caggtggcag gtctccaggg cccaggccag
      121 aaagaccagt tggtagccga ggatgtcggg gatctgcatc catctgagcg gactcaaacc
      181 ggtgaagaaa ctgagggcga ttgcatagta accggccccg aagacgaagc cggtaagcgt
      241 ggcttgaaag ccgttctggc agccgcgaat cagcaccaaa agtggtgcca gggcgaacca
      301 ggccaggtaa tcctggtcga aaccgggagt ggcgaaaccg agaactgccc ccatggcaaa
      361 ggcacagggc aatttgattg attgccttgc cgattcaagc cagcccgctt tcggggtgag
      421 gcttatgggg gtgaattgaa tttcccgact gaacatcacc ttcaaatgtt acacttaaat
      481 gagcttttca ttgcttttcc gggcgttata tttcggcctt aatttgaaac cagaagagaa
      541 aaccagacgc atcctgggaa tcgacccggg caccgccacc gtgggctttg gagtggtgga
      601 agtgagcggc atcaataagt ttgcctatat cgcttcgggg gtaatttcca ctcccaagac
      661 attgccgacc ggggcgcgct tgaaaatgat ccgagaagat attctttcga tttacgatga
      721 atatctacca cgggaagctg ccgtggaagc gcttttcttc ttcaagaatg ccaagaccct
      781 ggtgccggtt gcccaggccc ggggagttat tctggaagcc ctctcctgcc gcaatcaaat
      841 tccaggagaa tatacgccca tgcaggtgaa gctgcaaatt tccggttatg gcaaagccga
      901 gaaacacgag gtgcagtggg ccgtggccag actattgaac ttgaacgaaa tagtaaagcc
      961 cgatgatgct tccgacgcgc tcgctatcgc tgtttgtcat gcccgtatgg cttctttgcc
     1021 ggtttaatta gcggtagagt tcgcctaagg tacagagtgc caccaccttt ggggcgacta
     1081 ttaccgacag ggcggccagg gcaggcagca ttattcttct taagaaggag ctgcctttgc
     1141 cggctggcgg aaagcttttc accggaaggc ttttcaaagg ttccgccagt accgtcaggg
     1201 gcaagccggc cacggccccc gccgtcatga ttagattgac caagcgtccc agggtgtcca
     1261 ggtcgaaagc ctttgcctgg gcgagaaaat ctccctccag gggcatgagc ggatctgaga
     1321 tggtcatgag caagaatgcc tgccagagcc gcaaaggaaa gcagctccgc agaaagagaa
     1381 agcagagcgc ttgggtgagg aagtagacga ccattaccac cagtgcagtc tggcttatga
     1441 tgcggctcaa gtagcaagcg gtgatgccgc aggcggtaaa gagaaagcag aagtgcaggc
     1501 agagccagag aggcggtact ggcttttccg ctttctccgg gggcttcaga ttgtgaaaga
     1561 gataaacggc cacgaagagc gtataaagca ggggcggtag ataggagggt tcgtgcagag
     1621 cgctcaagag cggtaccaga cctgatgccg ccaggtattg cagcggtttc catttgctct
     1681 ttgtatttga ctcttcttca gggctcattt ccatctcaac ttacccggct gtaattcagc
     1741 cacgactcct ggtacctggc aggaaaagcc tgcgggtaga aaatttcggc aaggatctcc
     1801 agggagtcca taagccttgg tccggggcga ttgaagtact ggttgccgtc ggcaatatag
     1861 agcctctcgt ttttcactgc ttgcaggtca tgaaagcccg gtatattgca taaggcaggc
     1921 atttctttca gggtgcgctc gttatcgaaa ccgcagggag tgacgatgat tacatcgggc
     1981 tcccggcttt gcagcgaggc aaagtccatc cagggggaat gccgacctgc ttcgccgaag
     2041 aggtttatgc cccccgccat ttccaccagc tccggcatcc agttgcctgc tgccatcaaa
     2101 ggctctatcc attcgatcac tgctaccttg agcggtgcgc tgccgctttc gcacagatag
     2161 cgccgcgctt tctcttttac ttctgccagg cgcttgcgac tgacttcaat cagtgtttgt
     2221 gctgccgctt ctacctgtaa gcttgccccc accttttcca ggtcaagaaa gtagtcttcc
     2281 aggcaattag gcgaaaggga gattatttcg gggcggctgt ggataagctc gcaagcggct
     2341 gcttgcacat ccttgaggct gacggcgcaa acctcgcact gggcctgggt gagtacatgg
     2401 gtcggtgcca gttcgtccag ctttctggcg tccaccaggt agacgcttag cgatttctcg
     2461 agaatttctt tcacccgggc atcgatttct ttgctgctgc cgtccacgtt gaattttggt
     2521 gcagtgcata cgggcagtga ttttacggaa gccgggaagt cgcattcgtg ggagcgcccc
     2581 accatgtagt gaccaagtcc gagggcatga attatctcgg tggaagaggc aatcagtgat
     2641 actatccgtg gtgcggtcat agtctctctc cctgcccgcc ggcgaagaat gggctcttta
     2701 ccaggtctct ttctacatcc ttcaaactgg caactccagc cagttgcata gacagtaaca
     2761 gttcctcttg cagggtggaa agtaccgaac gcaccccgct ttccccctgc caggccagcc
     2821 cccagaggac cggacgaccg accaggacgg ctctggcacc gagagccagg gcctttagaa
     2881 tgtcggtgcc gcggcgaatg ccgccgtcca tcaagaccgt agtgtccggc acggcccggc
     2941 agatttcctc cagggcctcg atactggaga cggctgtgtc aagctgcctg ccgccgtgat
     3001 tggagacgat gatgccggct gctccggcgg ctaccgcctt gcgggcgtcg tcgcccctga
     3061 gaataccttt tacgaggacc ggaagcccgg cctctgccac aatccattcc agatcggcaa
     3121 agctgaggct caagtcgtag agggaggcaa tgtactcggc gagggcattt tgtccgcagt
     3181 ggccgctgat atcttgcagg aaaaagcgct ccagg
//
LOCUS       DAOQNZ010000306         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15133_length_3212_cov_4.027875, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000306 DAOQNZ010000000
VERSION     DAOQNZ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15133_length_3212_cov_4.027875"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PKW73_06760"
     CDS             <1..753
                     /locus_tag="PKW73_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73019.1"
                     /translation="LVLGTIEETMDAHRAVVEAVKSGRLSEARILASIKRLQKLFPER
                     LRTFNLDEEETRLKLFAREVENATASLVEISARAVVQHRQGFIRPEKNGLSQENTASP
                     LHATGTWHVLAPRHPRYPMNLADEIQRLEPRMQLSDWRYDLATDLEQFSNRLSSLEGE
                     KQVIFLTYRSFINKEQCKMMQDLLASCRVIHVAVDSPYDLVLSENSRETPSYALMDPS
                     NLAIQGLARVLTGRAKAEGRLNLDLSPVELPV"
     gene            832..1116
                     /locus_tag="PKW73_06765"
     CDS             832..1116
                     /locus_tag="PKW73_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73020.1"
                     /translation="MDQDSEAEKNTGSQNPQAPLSPEPAPAAASAPDSERERENDLLT
                     AGASLLEHPKVKTYIGRMSKIMVLFAILGCLMMIAAVTAITSLFNSIVPN"
     gene            complement(1163..2773)
                     /locus_tag="PKW73_06770"
     CDS             complement(1163..2773)
                     /locus_tag="PKW73_06770"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA73021.1"
                     /translation="MLRRTGPIPKSLQKLPAAPGLSEIHHYLSAAMEHRGKQVEISWT
                     TPDRLVDFTLNIHCPWRGGDTEWKLFVGKNMRLVWDYKSCDVLLVYNLIISSCGEVHQ
                     SIQADGALSVSEAFRDSSKKRDTYYMNSSNTVEESDPSSVAKVGTSDNAWSRSTLPPN
                     GDLSLVEISRLLQSVLLARMTGRLDIDHGSTHAKIFFVDGEPQFADVGGIAGDEGILD
                     VLTWREGKYKFEPRMRVTERNVGQPIETLVIQGVQLVDKVNFIKNVGLLPDSVLIRKH
                     QQLATTDLDNLSARDQTVDGALMRRFYQAIDDKTAIKVMVESLRLSRSQWVPIVASLL
                     GGDLVTFTNDFVGTREDKPSIAPKAIDKSKIHSVMMSLRRPETGMFTYPAFLYFLEQE
                     YFRGYRSGSPLSVMVMEMRVVSGPPHLSREPLDVPAVAEVVRRISRLKRHIDLLAHYD
                     QYDYAMLLPNTKTSGANIFAQRVARAIWSSPLAYGVDSANLSLAFGVACIPDDCLDLG
                     VLLAAAEAAKAQALRAAVPIALYRDLKG"
     gene            3019..>3212
                     /locus_tag="PKW73_06775"
     CDS             3019..>3212
                     /locus_tag="PKW73_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73022.1"
                     /translation="MSKADSSESFTFSAAVWIRDGVLIDRMPLNALAFAYCYQAILLK
                     QIHTNPHHTGLRTIPSISTLT"
BASE COUNT          836 a          903 c          801 g          672 t
ORIGIN      
        1 ctcgtgctgg gcaccataga agaaacaatg gacgcgcaca gggcagtggt agaagcagtc
       61 aaaagcggac gcctatcgga agcccgtatt cttgcttcca tcaagcgtct tcagaagttg
      121 tttccggaga ggctccgcac ctttaacctc gacgaggaag aaactcgact gaaactgttc
      181 gcacgggaag tcgaaaacgc cacagccagt cttgtggaaa ttagtgccag agcggttgta
      241 caacaccgcc agggatttat aagaccggaa aagaacgggc tatcacaaga aaataccgcc
      301 tcgcctttgc atgctaccgg cacctggcat gttctggcgc ctaggcatcc ccgctatccc
      361 atgaatttgg ccgacgaaat tcagagactg gagccgcgca tgcaattgtc ggactggcgc
      421 tatgaccttg ctaccgatct tgaacagttt tccaatcggc taagctccct ggaaggggag
      481 aagcaggtga tcttcctcac ctaccgttcc ttcatcaaca aagagcagtg caagatgatg
      541 caggacttgc tcgcctcctg ccgggtcatt catgtggcag tagatagtcc ttatgacctg
      601 gttctatccg aaaactcccg agagaccccg tcctacgccc tcatggatcc ctccaattta
      661 gccattcaag gtctggccag ggtcctgacc ggaagggcaa aggcggaagg acggctcaat
      721 ttagacctct ctcctgtaga gttgccggtt tagtcgacgg gcaagcctta tcttcgcctg
      781 gaagcacctg aaagaaagca ctataatgga tgcaccagcg ggagaagtgt aatggatcaa
      841 gattccgaag cagaaaagaa caccggctcc cagaatccgc aagcacctct ttctccggag
      901 ccggcgccgg ctgccgcaag cgcaccggac agtgaaagag agagggaaaa cgatcttttg
      961 acggccggag ccagcttact ggaacacccc aaagtaaaaa cctatatcgg ccgcatgtcg
     1021 aaaataatgg ttctctttgc catactgggc tgcctgatga tgattgcagc agtaacagcc
     1081 atcaccagtc tattcaattc catagtgccc aattaaagaa gccaggaaag gccggcacca
     1141 ccagcccctc cagcggtttc ccttaacctt tcaaatcgcg ataaagggca attggtacgg
     1201 ccgctctcaa agcctgagcc ttagccgcct ctgctgcggc caaaagtacg ccgagatcga
     1261 gacagtcatc gggaatacaa gccactccaa aggccagaga caggttggca ctatctacac
     1321 cataggccag aggcgaactc cagatggccc ttgccacacg ctgcgcaaat atattggcgc
     1381 ctgaagtctt ggtattgggc aagagcatgg catagtcgta ctgatcgtag tgggccagca
     1441 aatcaatgtg gcgcttcagt cggctgatgc gccgcaccac ctcggccacc gccggcacat
     1501 ccaacggctc tctggataga tgaggcgggc cggagaccac acgcatctcc atcaccatca
     1561 cggaaagcgg gctaccggag cgataccccc gaaaatactc ctgctcaagg aaatagagga
     1621 aggccggata ggtaaacata ccggtctccg ggcgccgcaa gctcatcatc acggagtgaa
     1681 tcttagactt atcgatggcc ttgggcgcaa tagatggctt gtcctcccgc gtgccgacga
     1741 agtcattggt gaaggtaaca agatcgccgc ctagcaaaga tgccacgata ggcacccact
     1801 ggctgcgact caaacgaaga ctctccacca tcactttgat agcagtctta tcatcgatag
     1861 cctgatagaa gcgcctcatc aaggcaccat ctacggtttg atcacgggca gaaagattat
     1921 cgaggtcggt tgtagccaac tgctggtgct tgcgaatcaa gacactgtca ggcagtaagc
     1981 ctacgttctt aatgaagttg accttgtcga caagttgcac gccctgtatg acgagagttt
     2041 caataggctg gccgacattg cgctcagtca cgcgcatgcg aggctcgaac ttgtactttc
     2101 cttctcgcca ggtaagtacg tccagtatgc cttcatcacc ggcgatacca ccgacatcgg
     2161 caaactgagg ctcaccatct acgaaaaata tcttggcgtg ggtgctgcca tggtcgatat
     2221 cgaggcgacc agtcatgcgc gccaatagaa ccgactgcaa tagtcttgaa atttccacaa
     2281 gagaaagatc gccatttggc ggcagcgtac tgcgactcca ggcattatcg ctggtgccta
     2341 ccttggctac ggaactgggg tccgactcct ccactgtatt ggaagaattc atataataag
     2401 tgtcgcgctt cttagatgag tcgcggaagg cttcggacac cgagagagca ccatcagcct
     2461 gaatgctctg atgtacttct ccgcacgagg aaataatcaa gttgtaaacc agcaagacat
     2521 cgcagctctt gtagtcccag accagccgca tgttcttgcc tacgaaaagc ttccactcag
     2581 tgtctccgcc acgccaggga caatgaatat tgagagtgaa atccacgagt cgatcaggtg
     2641 tggtccagga tatttctacc tgcttgcccc ggtgctccat ggcagcggaa aggtagtggt
     2701 gaatctccga cagacccggc gccgccggca acttctgcaa agacttgggg atcggaccag
     2761 ttctcctgag catacatacc atccttttca gcctgtatct acagcaatag gaaagggaac
     2821 acccacccta actcgataca gttaacgcct tactcgcctt ttattcccag accccaggaa
     2881 aactaacctg agacccggcg cagtcaagag attagtctat tatcagccac cacaatcagc
     2941 ctgcaagagt gctgcaaacc tggtagacaa taagcctgag gcgaggttcg aaagaccatg
     3001 cgggagcatt attaaccaat gagcaaggcc gattcaagcg aatcatttac attttcggcg
     3061 gctgtctgga ttcgggatgg ggtgcttatt gatcgcatgc ctctcaatgc cctggctttc
     3121 gcctactgct atcaggcgat cctcttaaaa caaatacata caaatccgca ccacactggt
     3181 ctccgcacca tcccgtccat cagcaccctg ac
//
LOCUS       DAOQNZ010000307         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15149_length_3210_cov_3.833914, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000307 DAOQNZ010000000
VERSION     DAOQNZ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15149_length_3210_cov_3.833914"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="eno"
                     /locus_tag="PKW73_06780"
     CDS             complement(<1..737)
                     /gene="eno"
                     /locus_tag="PKW73_06780"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNA73023.1"
                     /translation="MTHIEEISAMEILDSRGNPTVEVTVALTSGAMGRAAVPSGASTG
                     SFEAVELRDEDKARYHGKGVLKAIENIHDVIAENLIGMDAVDQTAIDHELLRLDGTDN
                     KAKLGANATLGVSLAVAKAAAEALGLPLFRYVGGVSANILPVPMMNILNGGKHAQDGV
                     DFQEFMIVPFGAKSFSECLRWGAEIYQALKAVLHKKGLGTGVGDEGGFAPSLKTNEAA
                     LELIMEAIEKAHLKPGSQIGLALDPAST"
     gene            1110..1478
                     /locus_tag="PKW73_06785"
     CDS             1110..1478
                     /locus_tag="PKW73_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73024.1"
                     /translation="MGNLHGGSFQAIQEYFQQKECRFCSHPFKADGIQLLREEPGVLV
                     VRVTCSTCGHPLGVAIVGTTPKTAKAQSTHPRDWTKRDRDRLVGLPPITYDDVLNAHQ
                     FFKSLDTDWRKHLPRVGKAS"
     gene            1621..>3210
                     /locus_tag="PKW73_06790"
     CDS             1621..>3210
                     /locus_tag="PKW73_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNA73025.1"
                     /translation="MAQTFNTETRLAERIRKEDPDLFVTWYFPNTYEIGMSGLGYQLV
                     WWLLEQDPALIVRRGFTDAEEPWQGLCDLLGFTVSWELDFINIISLLRKNDVSLTAKA
                     RLEESRERESRKDPPLPLVFGGGPVLTANPEPFHELFDVILLGDAETSVPSLVEAYRN
                     IKKEVGGDREQMLYLLSQVPGLYVPSQYTVEYSGQDNAPIKSISPIRAGVPPVVQRQN
                     FAPPSEYATHTQILSSATTWSERFLIEVVRSCPQECRFCLASYLTRPFRATPVDTLMT
                     KIEEALKHTSRIGLLGPSVTEHPLFDELAKRLAHRSDIDISIASVRADSLNENILATL
                     KALGQKTLTIAIESGSERLRTVMKKNLSEAEIFQAVELIYQAGFEGIKFYGIAGLPHE
                     RQEDLEQTVALMQRLKKKYRSLRFVFGVSSFVPKAQTPFQWHGRDRQSGKKLEYLRKN
                     LARLGIEVRPESHNWSDIQALISRGDRRLAPVLESVSGLGENLGAWKKALRNLPTGVP
                     NLDYYAFREIPWRQDGAILDNR"
BASE COUNT          823 a          899 c          781 g          707 t
ORIGIN      
        1 gtagaagcag gatcaagggc cagaccgatc tgggatcccg gcttcagatg agccttttca
       61 atggcttcca taatcagttc aagagcagcc tcgttagttt tgagcgacgg tgcgaaacca
      121 ccctcgtcac caaccccggt gccaagacct tttttgtgaa gcactgcctt tagtgcctga
      181 taaatctcgg cgccccagcg cagacactca gagaagctct tggcgccgaa gggaacgatc
      241 ataaactcct ggaaatccac accatcttga gcgtgtttgc cgccattgag aatattcatc
      301 atcggcacgg gaaggatatt ggcggagaca ccgccaacat atctaaagag cggcagtccg
      361 agagcctcag cggctgcctt tgcaaccgcg agactgacac caagggtggc attagcgcca
      421 agcttagctt tattatcagt accatccaat ctcaagagtt catggtcgat tgccgtttga
      481 tctacagcat ccataccaat gaggttctcg gcgatcacgt catgtatatt ttcgatagct
      541 ttgagtacgc ccttgccgtg atagcgggct ttatcctcgt cacgcaattc aaccgcctcg
      601 aagcttccgg tggatgcacc agatggaaca gcggcacggc ccatggcacc actggtgagg
      661 gcaaccgtaa cttcaacagt cggattcccg cgtgaatcca ggatttccat tgccgatatt
      721 tcttcaatat gcgtcattat ctttgcgctc ctgtttgctg acttaaattt ctcttctctc
      781 caaaatagct tggggagccg cggaacaggg aattatcgcc ctgcactgag cggtttatgt
      841 aaatcggtgt ccgcttttat caaagaaagt ttctcggaaa tgcgaatttc attgctgcga
      901 tttacaaagg ttcaagtata atcctttcac tagatttcca atttagatgc actaggaaag
      961 atcatcattg acgaaaatca ttgacaagat ccaaccgctc tggggctaag tctcaactaa
     1021 atcatctaat tgatcgcaat atagtcatcg tccttttgtg tccttctcag caattgttct
     1081 tcaaaagaac ctagaattgg cggaaaaaca tgggcaacct gcatggtgga agtttccagg
     1141 ctatccagga atatttccaa cagaaagagt gccgattttg ttcgcacccc tttaaagccg
     1201 acggcatcca actactgagg gaggagccgg gcgtgcttgt agtcagggta acctgcagca
     1261 cctgcgggca tcccctcggc gtggcaattg tcggtaccac acccaaaaca gcaaaagccc
     1321 agagcaccca tccccgcgac tggaccaagc gcgacaggga ccggttggtg gggcttccgc
     1381 ctatcaccta cgatgacgtt ctcaacgccc atcagttttt caagagcctc gacacagatt
     1441 ggcgcaagca tctgccgcga gtaggaaaag ccagctaggg caatcgtcaa gcaagacaag
     1501 aggaagaccg gaccaccagt ccggtctttt gctttctatg aaggatcgcc tctgaaatat
     1561 cagccccttg gcattttccg tccccgaggg gctatactca aggcaaaggc agcaaagtag
     1621 atggcgcaga ctttcaacac agaaaccaga ctggcggaac gaataagaaa ggaagacccc
     1681 gatcttttcg tgacctggta tttccccaac acctacgaga taggcatgtc cggactcggc
     1741 taccagcttg tctggtggct tctggagcaa gacccggctc tcatagtgag gcgcggcttc
     1801 acagacgccg aagaaccctg gcaaggtctt tgcgatttac tcggtttcac ggtttcctgg
     1861 gaactggact tcatcaacat aatttccctc ttgcgcaaaa acgacgtttc tctaactgct
     1921 aaggccagat tggaagagtc cagagagaga gaaagtcgca aagaccctcc cttacccctt
     1981 gtcttcggcg gcggcccggt gttgacagcc aatcccgagc cctttcacga gctcttcgac
     2041 gtcatccttc tcggtgatgc agaaacttca gtgccatcac tggtagaggc ataccgaaat
     2101 atcaaaaaag aagtaggcgg agatagagag caaatgctct atctactctc tcaggtaccc
     2161 ggcctatatg taccaagcca gtacactgtc gaatacagcg gtcaagacaa cgctcccata
     2221 aaatcgatct ctcccatacg cgccggagta ccaccggtgg tgcaacggca aaactttgca
     2281 ccaccaagcg aatatgcaac tcatacacag attctcagtt ctgccaccac ctggagtgaa
     2341 cgctttctga tagaggtggt gcgctcctgc ccccaggagt gccgattttg cctggccagc
     2401 tatctgacca ggcccttccg cgccacgccg gtggacacac tcatgactaa aatagaggaa
     2461 gcgctcaagc acaccagccg catcggactt ttgggtccca gcgtcaccga gcatcccctc
     2521 ttcgacgaac tggccaaacg ccttgctcat agaagcgaca ttgatatctc catcgcctcg
     2581 gtgagagccg acagtctgaa tgagaacatt ctcgccactc tcaaagcgct cgggcaaaag
     2641 accctgacca tcgccataga atcaggctcg gaaaggctca ggaccgtaat gaaaaagaac
     2701 ctgagcgaag ccgagatctt tcaagccgtg gagcttatct atcaggccgg atttgaaggc
     2761 atcaagtttt acggcatcgc cggccttccc catgaaagac aggaagacct ggagcaaacc
     2821 gtagccttaa tgcaacgcct caagaaaaaa taccgctccc tgcgctttgt cttcggcgtt
     2881 tcctccttcg tgcccaaagc gcagacccct ttccagtggc acggcagaga ccggcaaagc
     2941 ggcaagaaac tggagtacct gcgcaagaat ctagcccggc tgggtatcga ggtcagaccg
     3001 gagagtcata actggagcga tatacaggct ctcatttcca gaggcgacag acgcctggcc
     3061 cctgtgcttg agagcgtctc gggtctaggg gaaaatctcg gcgcctggaa gaaagccctg
     3121 cgcaacctgc ccaccggcgt accgaatctt gactactatg ctttccgaga gattccctgg
     3181 cgacaggatg gagctatcct ggataaccgt
//
LOCUS       DAOQNZ010000308         3206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15181_length_3206_cov_4.454142, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000308 DAOQNZ010000000
VERSION     DAOQNZ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15181_length_3206_cov_4.454142"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKW73_06795"
     CDS             <1..441
                     /locus_tag="PKW73_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73026.1"
                     /translation="IAQHGLEQSVVFNARPFSEIAMQLTRLLEGKQVLSWDLSSVQIL
                     FDELCVEYGQPPLVFSGHSLRDEYARFVGEVDAGGKNYKLQELKTNGPSAVAQCRALL
                     DTLYRMASSSQKTNNAETGNLGWTGQFYKPKLSPRDKIKDFLGF"
     gene            complement(451..1029)
                     /locus_tag="PKW73_06800"
     CDS             complement(451..1029)
                     /locus_tag="PKW73_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNA73027.1"
                     /translation="MKKILMIVGDFVEDYEAMVPFQILSMFGYQVDVVSPGKKSGEKV
                     KTAVHDFEGDQTYTEKRGHNFAVTANFSRLSADNYAGLVLPGGRAPEFLRLNEEVLDI
                     VRAFFQAQKPVAAICHGLQILTAAGVLKGRSCTAYPSVKPEITAAGGIFVAVNEKADN
                     SYVCGNLVSAPAWPAHPRWMKDFLTLLGHKIG"
     gene            1071..2237
                     /locus_tag="PKW73_06805"
     CDS             1071..2237
                     /locus_tag="PKW73_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNA73028.1"
                     /translation="MKRVETAIVIGGGIAGLTTALALGRLGIDVKVFEQADSLREVGA
                     GLTVWSNGMYALDELGAAHDVLKAGQVVESFRFLDEAGQVLGAVDLARLERLVGQPSV
                     TIHRRALLHALAQVLERTVSGCSIHLSEKFLRYRQEGGQVAAYFESGSVHEADILIGC
                     DGFNSLVRKQVLGDTDRNYSGYTCFRGVTEFPEAEIPRGAVWHTNGVGTQFGLLHTGA
                     GQMAWYATANMPSGVIEDAGERKDYLLDRFGSFHELVPRLLGATDSSAILKNDIYDRS
                     PIYKWSDASVLLVGDAAHPTTPNLGQGACMALEDAAVLGRAVKGGGSVEDIFDRFCKR
                     RMHRTSFVTRSSRRAGAFNQSENQWLLKCRDKLTAALFKGGSMPTIDKIMGYKI"
     gene            complement(2234..2971)
                     /locus_tag="PKW73_06810"
     CDS             complement(2234..2971)
                     /locus_tag="PKW73_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73029.1"
                     /translation="MTNFSEGQENILDQVFLASPCEARWEDMAGDDKVRHCELCSLNV
                     YNIEAMTTREAQEFFAAGKDGRRICARFYRRQDGTIITSDCPRGLKAIRDGYQKVYRR
                     VAAFLTLFLGLGGSAIAQNQRREMPVKPGKIMMVQPWNATESSTPAERALQEKLASQM
                     EKEKTIFSKERVNLLLQLAQVYRQEKAATKDESQQCHYKGYAQICFNSALSIAQNLKD
                     QALTKQVESKMKEANAADESSAGNKKD"
BASE COUNT          688 a          824 c          867 g          827 t
ORIGIN      
        1 attgcccagc atggtcttga gcagtctgtg gttttcaatg ccagaccgtt ttcggaaatc
       61 gccatgcaac tgactcgcct tttagagggt aaacaggttc tttcctggga tttaagctcc
      121 gtgcagatcc tatttgacga actttgtgtg gagtacggac aaccgcctct ggtcttcagc
      181 ggacacagtc tcagagatga gtacgcgcgc ttcgtaggtg aagtagatgc cggcggcaaa
      241 aactataaat tgcaagagtt gaagacaaac ggtcccagtg ctgtggcgca gtgccgggcc
      301 ttgctggaca cactttatcg catggcttcc tcttctcaga agaccaataa tgctgagacc
      361 ggcaacctgg gctggaccgg acagttttac aagcccaagc tatcaccccg ggacaagatt
      421 aaagacttcc tgggctttta agtattgcct tcagccgatt ttgtgcccca ataaggtgag
      481 gaagtccttc atccagcgag ggtgggcagg ccaggccgga gcactgacca ggttgccgca
      541 gacatagctg ttgtcggctt tctcattgac tgccacaaag atgccgccgg ctgctgttat
      601 ttcaggtttt acggacggat aggccgtgca acttctgcct ttgagaacac cggcggcagt
      661 tagaatctgt aagccatggc aaatggctgc caccggtttt tgcgcctgga agaaggctct
      721 cactatgtcc agtacctctt cattcaagcg caggaattcc ggtgcccgcc ctccgggcag
      781 aaccaatccc gcataattgt cggcggatag cctggaaaaa ttggcggtaa cggcgaagtt
      841 atgaccgcgc ttttcggtgt aggtctggtc gccttcgaag tcatgcaccg ctgtcttcac
      901 cttctcgccg cttttcttgc cgggtgagac cacatcaact tgatagccga acatggaaag
      961 aatttggaaa ggcaccatgg cttcatagtc ttctacaaag tcgcccacaa tcatcagtat
     1021 cttcttcata tttctctacc tttgctgccc tatggaagga taatatcccc atgaaaaggg
     1081 tggaaacggc aattgttatc ggcggcggta ttgcggggtt gacaacagcc ctcgctcttg
     1141 gccgactggg cattgatgtg aaggtgttcg agcaggccga tagtttgagg gaagtgggag
     1201 ccggattgac ggtctggtcc aacggtatgt atgcccttga tgaacttggt gcggctcacg
     1261 atgtgctaaa ggccgggcag gttgtggaga gctttcgctt tctggacgag gctgggcaag
     1321 tgctgggggc cgtggatctg gccaggctgg aacgtcttgt ggggcagccc tcggtcacca
     1381 tacaccggcg ggcgctgcta catgcactcg ctcaggtact ggagcgaaca gtttccggct
     1441 gcagcatcca tctttcagaa aaattcttgc gctacaggca ggaaggtggg caggtcgctg
     1501 cttactttga aagcggtagc gttcatgagg ccgatatcct gattggttgc gacggcttca
     1561 attcccttgt gcgcaagcag gtcttgggag acaccgatcg caactatagc ggctacactt
     1621 gctttcgcgg cgtcaccgaa tttcccgaag cagagattcc ccgcggcgca gtctggcaca
     1681 ccaatggcgt cggtacgcag tttggactct tgcacaccgg agccgggcaa atggcctggt
     1741 atgccacggc taacatgccc agcggtgtaa tagaagatgc aggcgaaaga aaagactact
     1801 tgctggatcg ctttggttcc tttcatgagc tggtgccgcg ccttttaggc gccaccgata
     1861 gtagtgccat actgaaaaat gatatttacg atcgctcccc tatctataaa tggagcgatg
     1921 cttctgtttt gctggtaggg gatgccgccc atcccaccac tccaaacctt ggccagggcg
     1981 cctgtatggc cctggaagac gcagccgtcc tggggcgggc tgttaagggc ggcggaagcg
     2041 ttgaggatat ttttgaccgg ttttgcaagc ggcgcatgca ccgcacttcc tttgtcactc
     2101 gaagttccag acgagccggt gccttcaacc agagtgagaa tcagtggttg cttaagtgtc
     2161 gcgacaagtt gacggcagct ctcttcaaag gcggtagtat gcccaccatc gataagatta
     2221 tgggctacaa gatctagtct ttcttgtttc cggcactgct ttcgtcggcg gcattggcct
     2281 ctttcatctt gctttccact tgcttggtaa gagcctggtc tttaagattt tgagcgatag
     2341 atagagccga attgaagcag atctgggcat accctttgta atggcattgt tggctctcgt
     2401 ccttggtggc tgccttctcc tggcggtaga cctgggctaa ctgcaagagc aaattgacgc
     2461 gctctttaga aaaaatggtt ttctcttttt ccatctgact tgccagtttc tcttgtaagg
     2521 cccgttcagc cggggtagag ctttctgtcg cattccatgg ttgcaccatc atgattttgc
     2581 ctggtttaac cggcatctct cttctctggt tctgggctat tgccgaaccg cctagaccga
     2641 gaaagagggt gagaaaagct gccacgcgcc ggtacacttt ctgataacca tccctgatgg
     2701 ctttcagccc cctggggcag tcactggtga tgattgtgcc gtcctggcgc ctgtagaagc
     2761 gagcgcagat gcgcctgccg tctttaccgg ccgcgaaaaa ctcctgggct tccctggtgg
     2821 tcatggcttc aatgttatag acattgagag agcaaagttc gcaatgcctg actttgtcat
     2881 cgcctgccat atcttcccag cgagcctcac aggggctggc tagaaaaacc tggtccaaaa
     2941 tattctcttg cccttccgaa aaattagtca taaggcaata cttccttttc tttgctcagg
     3001 accggcagat tatagctgat aactccttcc taattttgaa aaaatcgagg aagaagtaag
     3061 tgcatcacaa tgactccagg cagaggctcc ccttgttcaa gaaattgctc gctacccttg
     3121 ccgttttcgg cttttccagt accgctcttg cctttgctta tgtgagcgat tatagcccat
     3181 tgtcattaaa agggctgtgg cggtat
//
LOCUS       DAOQNZ010000309         3200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15243_length_3200_cov_3.212083, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000309 DAOQNZ010000000
VERSION     DAOQNZ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15243_length_3200_cov_3.212083"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKW73_06815"
     CDS             complement(<1..246)
                     /locus_tag="PKW73_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73030.1"
                     /translation="MPGFETDDKSKPRKVEPSPFEAVLNGGEKLNGPTGPQQPLDANK
                     KQEPAPVAKQGDGVTAPAKPPGEADKTATTPDKTATTP"
     gene            complement(438..2852)
                     /locus_tag="PKW73_06820"
     CDS             complement(438..2852)
                     /locus_tag="PKW73_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNA73031.1"
                     /translation="MNIQERSLRSLEWDRLIQELSLLAETDAGRLRLLALAPEQESVP
                     LAVARVLLSETTEAKAIISGRGEPALGGLNTSFESLDSLAVGARLSAPELINLEKLLR
                     ISRLTRSSFNLLAPESFPCLEPIAELLFARPVLEKDITLALEPDGTVKDSASHLLQRL
                     RQDVRGLEERVKEELRSLIHRLAGSRALTDELYTMRHGRYVLPVNASMRYQVEGIVHD
                     ASGSGLTVYVEPLSVMELSNQIRLKQSEIEAEIERILAALSDSLRPFANNLRESLNSL
                     IELDCIFARGRLAVKMQGESVELTTEPVLELKQARHPLLVLQGGDRQVVANDVSLGGD
                     RRALIITGPNTGGKTVLIKLVGLSALMVGFGLLPPLGRGSRLGLFDQVLSDIGDDQSI
                     QESLSTFSSHMKNMVEILAAAHSGKRILVLLDEIGAGTDPREGAALSQAILEELQTAS
                     ATVIASTHLGSLKTLAFRDNSFINGSFEFDEENLKPTYRLKLGVPGQSKATEVAGRLG
                     LTAPVMARAQELLRSEKDGLDKVVEELSLKLSSVKQHELDLQDLRAELEQRLAGLSAR
                     EKELDKSRRAVQEEERARLLADFEAARELVRQTVAQLQRQPDLKKAEQARRDLEGIKK
                     ALKWQEREPLPETAAQIAAGTRVRHLVLNRTGVVLELVGETCLLQLGNMKVKARLGEI
                     ELLDKTVSAGRQANAGQARGQKHKPAFQAAEPAGVSVFVRTMANTLDLRGKRVDEALG
                     FLDSFVDGCVLGRISPFMIIHGHGTGAVKSAVRDFLKAARYAIKFRPGEQYEGGDGVT
                     VVEITD"
     gene            complement(2854..>3200)
                     /locus_tag="PKW73_06825"
     CDS             complement(2854..>3200)
                     /locus_tag="PKW73_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73032.1"
                     /translation="LSPGCFTRSFTMPVFEQPMRKLLGTLSPTTTWLNEKGIVDVSAV
                     PEDHLEAIKQLERIGVVHKRNNRSYELQKIRLKKLLGNLGIESLASDYPAAAVLPTQT
                     TGHNEAHLTELN"
BASE COUNT          682 a          940 c          864 g          714 t
ORIGIN      
        1 cggtgtggtg gcagtcttgt ccggtgtggt ggcagtcttg tcggcctcac ctggcggctt
       61 ggccggtgcg gtcacgccat cgccctgttt tgctaccggt gccggctctt gtttcttgtt
      121 ggcatcaagg ggttgctgcg gaccggttgg cccattcagc ttctcgccgc cgttaagtac
      181 agcctcaaag ggagaaggtt ccaccttgcg cggcttgctt ttatcgtccg tttcaaaacc
      241 tggcatgcct gaaagacctc aagaaactag ttacaccaac gcattgatta atttattcca
      301 gtaaagccag tctttgaaac tggggaaact acgtaccagt gatgtcaagc ggactttttt
      361 aataacatct cggtcgcctt aaatttcaaa atgcgctttc ggttatatac ggagccgccg
      421 ttttctggac aataaattca gtcggtgatc tccaccacag tcacgccgtc cccaccctca
      481 tattgttcgc cgggtcgaaa cttgatggca taacgggcgg ccttcaaaaa gtctctcact
      541 gccgatttaa cagcccctgt gccatgacca tggatgatca tgaaggggct tatgcggcca
      601 agcacacagc catccacgaa actgtccaaa aaaccgagcg cttcatccac tcgcttgccg
      661 cgcagatcaa gggtgttggc catggtgcgc acaaagaccg acactccggc cggctccgcc
      721 gcctgaaagg caggtttatg cttttgaccc cgagcctgac cggcattagc ctgtcgcccg
      781 gcgctcacag tcttgtcgag aagctcaatt tctccaagcc tggccttcac tttcatgttg
      841 cccagctgca aaagacaggt ctcaccaacc agctcaagaa ccacaccggt gcggtttaag
      901 acaagatgcc tcaccctggt gccggcagca atctgggcgg cagtctcggg caaaggctct
      961 cgctcctgcc acttgagggc ttttttaatt ccttccaggt ctcgcctggc ctgttcagcc
     1021 ttcttaagat cgggctgtct ctgtaactgc gctacggtct gtcgcaccag ttcccgggct
     1081 gcttcaaaat cggccagaag cctggctctt tcttcctctt gcacggcccg ccgcgactta
     1141 tccagctctt tttcccgggc ggaaagaccg gccagtctct gctccagttc cgctctcaag
     1201 tcctgcaggt ccaattcatg ctgcttgacc gaggataact taaggctcag ttcctccacc
     1261 accttatcga gcccgtcctt ctcactgcgc aaaagttcct gtgccctggc catcaccggg
     1321 gcagttaaac caaggcgacc ggccacttcg gtagccttgg actggccggg cacgccgagc
     1381 ttaagacggt aggtgggctt gaggttctct tcatcgaact cgaaactgcc attgatgaaa
     1441 gagttgtcgc gaaaagcaag ggtcttgagg ctacccagat gggtggaagc aatgacggtg
     1501 gcagaggcgg tctgcaactc ttccagaata gcctgggaga gggctgcgcc ttcacgggga
     1561 tcggtgccgg ctccgatttc gtccaacagc accaggatgc gcttgccgga gtgggcggcg
     1621 gcaagaatct ctaccatgtt cttcatatgg gaagaaaagg tggaaaggct ttcctgaatg
     1681 gactggtcat caccaatatc cgagagcacc tggtcgaaca gccccaggcg ggagccccgc
     1741 cctagaggcg gtagaagacc aaagcccacc atcaaagcgg aaagccccac cagcttaata
     1801 agcacggtct tgccgcccgt attggggccg gtgataatga gagcccgccg gtccccgccc
     1861 aggctgacat cgtttgccac cacctgacga tcgccgccct gcaacaccag gagcggatgg
     1921 cgcgcctgct tcaattccag cacgggctcc gtggtcaact ccacagattc gccctgcatt
     1981 ttcacagcca ggcgcccgcg ggcgaagatg caatcaagct ctataaggct gttcaaactt
     2041 tcccggagat tattggcaaa cggtctcaag gagtcgctga gagcagccag aatacgctct
     2101 atttctgctt cgatttcgct ttgtttgagc ctgatttgat tggaaagttc catcaccgaa
     2161 agcggttcta catagacggt gagaccgctg cccgaggcat catgcactat gccttccacc
     2221 tggtagcgca tggaggcatt gaccggcagc acataacggc cgtggcgcat ggtatagagt
     2281 tcatcggtga gggcgcggct accggccagc cgatgaatca aggaacgcag ttcttccttg
     2341 accctctctt ccagaccgcg cacgtcctgg cgcagcctct gcaggagatg actggccgaa
     2401 tccttgaccg taccgtccgg ttcaagggca agcgtaatat ctttctccag cacggggcgg
     2461 gcaaaaagca actcggctat gggctccaag caggggaaac tttccggcgc cagaagattg
     2521 aaactggatc tggtcagacg cgagatgcgc agaagctttt ccaggttgat aagctccggg
     2581 gcggacagtc tcgcccccac agccagactg tccaggcttt caaaagaggt gttcagtcca
     2641 cctaaagccg gttcgccccg accactgatg atcgctttcg cttccgtggt ttcggaaagc
     2701 aagacccggg ccactgccag gggcacgctc tcctgctcgg gagcaagagc caaaagtcgc
     2761 aagcgaccgg catcggtctc agccagaagc gaaagctctt gtattagcct gtcccattcc
     2821 agagacctga ggctgcgctc ctggatattc aaactagttg agctcggtca gatgagcctc
     2881 gttatgcccc gtggtctgag tcggcagaac cgctgcagcc ggataatcgg aggccaggct
     2941 ctctatgccg agattcccga gtagtttctt gaggcgaatt ttttgcagtt cgtagctgcg
     3001 attgttgcgc ttatgcacga cacctatgcg ctccagttgc ttgatggctt ccaggtggtc
     3061 ttccggaaca gctgagacat cgacgatgcc cttctcgttg agccaggtgg tggtgggaga
     3121 cagggttccc agtaatttgc gcatcggctg ttcgaaaacc ggcatagtaa aactcctcgt
     3181 aaagcaacca ggcgaaagcc
//
LOCUS       DAOQNZ010000310         3195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15290_length_3195_cov_3.433439, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000310 DAOQNZ010000000
VERSION     DAOQNZ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15290_length_3195_cov_3.433439"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKW73_06830"
     CDS             complement(<1..545)
                     /locus_tag="PKW73_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73033.1"
                     /translation="MQGTLEKGDWRADYERKKCSAEEAVRVIQSGDHVYVHSNAAVPE
                     CLVDAMVGRASELRDVTVLHLLTLGKAEYAQEQYQDSFRVKALFIGKNIRDAVNEGRA
                     EYMPIFLSEIPRLFESGTVPVDVCLIQVSPPDAHGFCSYGVSVDCTIAARKKARFVLA
                     EVNKQMPRTLGRSFVHVSRLDR"
     gene            complement(559..1875)
                     /locus_tag="PKW73_06835"
     CDS             complement(559..1875)
                     /locus_tag="PKW73_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNA73034.1"
                     /translation="MTEEKETRRIVIPEVTRVEGHSAVTVDIKDGAVVDVKLDVFEGT
                     RFFERIVVGHKADEMAHLTSRICAICSTGHVLASIRALEATVQYKPSVRLNLFRELMH
                     LGMIIESHATHLCALVLPDFLGTKDLLTFADKFKEEFALWTTLRSLGCAIQTLVGGRP
                     FHPVNLHVFKLSHYPSRESLESLLPDLKSGADLALKLAHTFLSLKLPNIPSLEPNYLC
                     LEQDGVYGYFGDHIRSSEGFRRPISEYKEYLSEYAVTYSHAKKSRSNGKPYMVGAIAR
                     LHFYHELLGSTARSIYMASDLARGSCNSMLNNLAQAIELVQAIERVQTIIALLSELSH
                     EPEVEVPEPRLVGSGVGAVECPRGTLYHSYEVDEQGVVIAADMITPSAQNTERIERDI
                     RNTVSHRLAENGAGKDENLECDLETLIRSYDPCNTCATHAVRLRYV"
     gene            complement(1877..2680)
                     /locus_tag="PKW73_06840"
     CDS             complement(1877..2680)
                     /locus_tag="PKW73_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73035.1"
                     /translation="MNLESKHNTLPTIAVEGLTGCAGELLVILENLDKLLPFVRVVSF
                     PFAQSSNQGGPVDITFVEGSVSQKHDLEKLERIRAASRWLVAVGNCAVWGCVQKGGCT
                     NCSAAEKMLQVYGKDADFDVLEAAPLEKHVHVDVRLSGCPINATQFLSVTASLLQDHL
                     PYITSKPVCLECKLKENPCVLIEDNLPCLGPVTAAGCGALCPSMGAACYGCWGPCQSP
                     QMDAMAQVLTEKGYALEEVISRLGSFGAPAELFKRWKPFNENGAAVKGV"
     gene            complement(2690..>3195)
                     /locus_tag="PKW73_06845"
     CDS             complement(2690..>3195)
                     /locus_tag="PKW73_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73036.1"
                     /translation="GLGVAPIRSVWQYALDHREDFGKLILIYGMRHTTDILFRQELKF
                     LLRRRDIDIFIGAEEVMGPELPPIAAQLGRVTDMLRLADISPDYCVAVCGPPVMYKYV
                     IAGLKQKGVPDEQIWLSLERHMKCGIGKCGHCFIGGKFNCKAGPVFALPEIKHAPEAI
                     ECANGEY"
BASE COUNT          767 a          876 c          867 g          685 t
ORIGIN      
        1 cgatcgagac ggctcacatg cacaaaagaa cggccaagag tgcggggcat ctgcttgttg
       61 acttcagcca ggacaaagcg ggctttcttg cgcgcggcga tggtgcagtc tacggagacg
      121 ccgtagctac agaagccatg agcatccggg ggcgaaacct gaatcaaaca gacatccacg
      181 ggcacggtgc ccgattcgaa caggcgagga atttcgctca agaagatggg catgtattcg
      241 gcccgcccct cattgacggc gtcgcgaata tttttgccga tgaagagagc tttgacgcga
      301 aaagagtctt gatactgctc ctgggcgtat tcggccttac ccagggtcaa aagatgcaaa
      361 acggtgacat cgcgcagttc ggaagcacga cccaccatgg catcgaccag gcattcgggc
      421 acggccgcat tggaatggac ataaacatga tcgccgctct gaataaccct tacggcttct
      481 tccgccgagc acttcttcct ttcatagtcg gcgcgccaat cgcctttctc aagagtacct
      541 tgcataactt accttcaatt agacgtaacg cagccgcact gcatgtgtag cacaagtatt
      601 acacggatcg taagagcgga tcaaagtttc cagatcgcac tccaggttct cgtccttgcc
      661 ggctccattt tccgccaacc ggtggctgac cgtattacgg atatcacgtt cgatacgctc
      721 ggtattctgg gcggaagggg taatcatgtc ggcagcgatt actacaccct gctcatccac
      781 ctcataggaa tgataaagcg tgccccgggg acactccacc gcccccacgc cgcttccgac
      841 caggcggggc tcaggcactt ccacttcggg ctcatggctc aattccgaca agagggcgat
      901 tatggtctgc actctttcta tagcttgcac aagttcgatg gcctgagcca gattattgag
      961 catggaattg caggagccac gcgccagatc ggaagccata tagatagatc gggccgtgct
     1021 gcccagaagc tcatgataaa aatgcagccg ggctatggca ccaaccatgt agggtttgcc
     1081 gttggagcgc gattttttag catggctata ggtgacggcg tactcgctca aatactcttt
     1141 gtactcggaa atggggcgac ggaagccctc cgaggagcgt atatgatcgc cgaaatagcc
     1201 atagacgccg tcttgctcca ggcagagata gttgggttca aggctgggaa tattcggcag
     1261 tttcagactt aaaaaagtat gggccagctt caaagccagg tcggcaccgc tcttcaagtc
     1321 gggaagcaaa ctctccagag actctctcga tggataatga gagagcttga agacatgcaa
     1381 gttgacaggg tggaaagggc gaccacccac cagggtttga atggcgcaac ccagtgagcg
     1441 cagggtcgtc cagagggcaa actcttcttt gaacttatcg gcgaaggtaa gcaggtcttt
     1501 tgtaccgaga aagtcgggca agaccagggc gcaaagatgg gtggcatgag attcgatgat
     1561 catgcccagg tgcataagtt cgcggaaaag gttgaggcgc accgaaggtt tgtactgcac
     1621 cgtcgcttcc aaagcacgaa tggaagcgag gacgtgaccg gtcgagcaga tggcgcagat
     1681 gcgggaggta agatgggcca tttcgtcggc tttgtgccct acgacaatgc gctcgaagaa
     1741 acgagtaccc tcgaagacat ccagtttgac atcaactacg gcaccgtctt tgatatcaac
     1801 ggtgacggcc gaatgtcctt ccactcgggt tacttccggt atgactatgc ggcgtgtttc
     1861 tttctcttca gtcacctcag acccccttta cggctgcgcc gttctcattg aaaggtttcc
     1921 agcgcttgaa gagttcggcg ggcgcgccga aacttcccag tcgcgaaatc acttcctcaa
     1981 gagcgtatcc tttctcggtt aagacctggg ccatggcatc catttgggga gattgacagg
     2041 ggccccagca gccatagcaa gccgcaccca tactggggca gagggcgcca caaccagcag
     2101 cggtaaccgg accgaggcag ggcaaattgt cttcgatgag aacacagggg ttctctttca
     2161 gtttgcactc caggcagacc ggcttactgg ttatataagg taagtgatct tgcagcagac
     2221 tggctgtcac cgagagaaat tgggtggcat tgatggggca gccggagagg cgcacatcca
     2281 catgcacgtg cttttccaga ggggctgctt ccaatacatc gaagtcggca tctttaccgt
     2341 agacctgtag catcttctcg gcagcactgc aattggtgca accacctttc tgcacacagc
     2401 cccagacggc gcagttgcct actgccacca accagcgact ggcggcgcga atacgctcca
     2461 acttttccag gtcatgcttc tgactgacgc tgccttctac aaaggtgata tccaccgggc
     2521 cgccctggtt ggaagactgg gcgaaaggaa aactaaccac cctcacaaag ggcagaagct
     2581 tgtccagatt ctccagaata acgagcagtt caccggcgca gccagtcaag ccttccaccg
     2641 ctatggtggg cagagtattg tgtttacttt ccagattcat actcacggct caatattctc
     2701 catttgcgca ttcaatggct tcgggcgcat gtttgatttc aggcagggca aaaaccggtc
     2761 ccgccttgca attgaatttg ccgccgataa aacaatgacc gcacttgccg ataccgcact
     2821 tcatatggcg ttccagtgag agccagatct gctcatcagg aacgcctttc tgcttgagtc
     2881 ctgcaatcac atatttgtac ataaccggcg gaccgcagac ggctacgcag taatcggggc
     2941 ttatgtcggc caacctgagc atatcggtga ccctgccaag ctgggcggct attggcggca
     3001 gttccggccc catcacttcc tcggcaccga tgaaaatgtc gatgtcccgg cggcgcaaga
     3061 gaaacttcaa ttcttgccga aaaagtatat cggtggtgtg gcgcatacca tagatcaaaa
     3121 tgagcttgcc gaaatcctcc cggtgatcga gggcgtattg ccagaccgaa cgaatcggcg
     3181 ccaccccgag accgc
//
LOCUS       DAOQNZ010000311         3190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15332_length_3190_cov_3.808293, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000311 DAOQNZ010000000
VERSION     DAOQNZ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15332_length_3190_cov_3.808293"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /gene="nadE"
                     /locus_tag="PKW73_06850"
     CDS             <1..1666
                     /gene="nadE"
                     /locus_tag="PKW73_06850"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNA73037.1"
                     /translation="DSALTLALALHAAREPDSPVKRVHAALLPYFIEAGTSRQDDATK
                     LGQEVLKGFNHPKLTSSLIDLSESHGVLKKAVDKALDIPGDAWAAGQLVSYLRTPALF
                     YITSLLTQSGYPSILVGTTNRDEGGYIGYFGKAADAMVDLQLISDLHKSEVYELARAF
                     EIPAQVLQEAPTGDIYDGRTDEELIGVPYDFIELYSLYLAQPDRRAVSYLLENLDREG
                     QEQFEFLSKRLNKLNSQNAHKYASGSQSIHMDVMERAVPGGWRQDIDQEALKAGMTGG
                     NFVNLFQLPEQFLSPLQKRVWQVPTTRAEPLQRENIADYEDSAFILKQVMNQAECAAF
                     LHELDQLEWVPVGLNGMRKDYKEGDKIGSYRASLFSEPLAHCLFERIASAFPLLRIMD
                     PFTPADFDGAPVWRACGINPLMRFIKYEVGGSLVPHYDAPFDYYDGKRTIMSMVLYLT
                     QSKPEDGGATRFILEPQVNIPPEERNYEDWKREAGPEEIVAIASPGAGDALFLDHRVL
                     HDGQLLNAGRKIIMRTDVIFERCGLPPRRKPGVSKPLGMPERQGYD"
     gene            1659..>3190
                     /locus_tag="PKW73_06855"
     CDS             1659..>3190
                     /locus_tag="PKW73_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73038.1"
                     /translation="MTEEFNEEKARRLIEKALQDPIYSRALPYLRGADDLQTARALDL
                     AGFFDDGGEPEDRHFRADENWIMTPLSKLIPPLERFLKVAKANLEEPPPPPVVLLSTG
                     AFCPIHSGHLEMMEIARRSLEARGRTVIGGYLSPSHATYVDPKCGSETLSSSHRLMLC
                     QEAVKDSDWLMACGWEALGVERMLNFTDVIIRLERYLENQLAKSDLARRADKSRTNRT
                     TYGPAPVVYYVFGSDNARFTRGFLPDGKCVCVLRPGSEEPYHEFACAPLVQANSEIIF
                     AGEASHDLSSQSIRRRRSAQTSFHSAPASYFHWQTQSEGDQPAATIYLRDEGLWSLSS
                     FPLPEETLSTALDKFKEGLLALMIECHERASWPDTKRQLQVELLSLSEQRQAAQKLAR
                     GHSIISLDPCIEGDFNLEISRCYPLNEPLATPTMVARPGAASIVKQVGLIPPGQYILF
                     DDDIFSGSTVRQVQDLLPDSIAIRAVCALTIRGTSKHTVLDILDLRDFLAGARLAGLV
                     MST"
BASE COUNT          738 a         1008 c          814 g          630 t
ORIGIN      
        1 agactcagcg ctaaccctgg cccttgctct acatgccgcc cgggagccgg attcaccagt
       61 caagcgcgtc cacgccgcct tgctgcccta tttcatcgag gccggtacca gcaggcagga
      121 cgacgccaca aaactcggtc aggaggttct gaagggcttc aatcacccca agttgaccag
      181 ttctctcatt gacctcagtg aaagccatgg ggtcttaaaa aaagccgtgg ataaggccct
      241 cgacataccc ggagacgcct gggcggccgg tcaattggtt tcctacctgc gtaccccggc
      301 ccttttctac atcaccagtc tcctcaccca gagcggctat ccgtccatcc tggtcggcac
      361 caccaaccgc gatgaaggcg gttacatcgg ctatttcggc aaagccgccg atgccatggt
      421 agatctgcaa ctgatttccg acctgcacaa aagtgaggtc tacgaactgg ccagagcctt
      481 cgaaattccc gcccaggttc tgcaagaagc acccacaggc gacatttacg atggtcgcac
      541 cgacgaagaa ctaattggcg ttccctacga cttcatcgaa ctatacagcc tctacctggc
      601 tcagcctgac cggcgggccg tttcctacct gctagaaaac ctcgaccggg aagggcaaga
      661 acagtttgag ttcctctcaa aacggctcaa caagctcaat tcacaaaacg cccacaagta
      721 tgcatcgggc tcccaatcca tccacatgga cgtaatggaa agggcagttc ccggcggctg
      781 gcggcaagac atcgaccagg aagcgctaaa ggcaggtatg accggcggca acttcgtcaa
      841 tctcttccaa ttgccggagc aatttttgag ccccctgcaa aagcgcgttt ggcaggtccc
      901 aaccacgcgg gcagaaccct tgcagcgaga gaatattgcc gactacgaag actccgcctt
      961 catattgaaa caagtaatga accaagccga gtgcgccgcc ttcctccatg agctggatca
     1021 gcttgaatgg gtgcctgtag gcttgaacgg catgcgcaaa gactacaaag aaggcgataa
     1081 aatcgggagc taccgcgcct ccctttttag cgagcccctg gcccattgcc tctttgagcg
     1141 catcgccagc gcctttcccc tgctgcgcat catggaccct ttcaccccgg ccgactttga
     1201 tggagccccg gtctggcgcg cttgcggcat caatcctctg atgcgcttta tcaaatacga
     1261 agtgggcggc agcctggtac ctcactacga tgcccctttc gactactatg acggcaagcg
     1321 caccatcatg agtatggtgc tctaccttac ccagtcgaag cccgaagatg gcggcgccac
     1381 ccgcttcata ctggagcccc aggtcaatat cccacccgaa gagcgcaact atgaagactg
     1441 gaagcgcgag gccggaccgg aagaaattgt agccatcgcc tccccgggag cgggggatgc
     1501 cctcttcctc gatcaccggg tactgcatga tggacaactg ttgaatgccg gtagaaaaat
     1561 catcatgcgc accgatgtca tttttgaacg ctgcggtctg ccgccccggc gaaaaccggg
     1621 tgtctccaag ccgctgggta tgccggagcg ccagggctat gactgaagaa ttcaatgaag
     1681 aaaaagccag gcgactcatt gaaaaagcgc tgcaagatcc aatttacagc cgcgccctgc
     1741 cttatttgag aggcgcggat gacctgcaga ccgcccgcgc ccttgatcta gccggttttt
     1801 tcgacgacgg cggcgagccc gaagaccggc atttccgggc cgacgaaaac tggatcatga
     1861 cgccgctttc caaactgata ccacccctgg agcgtttcct caaggttgcc aaagccaatt
     1921 tagaagagcc gccgccacct cccgtggtgc tactgagcac cggcgccttc tgcccaattc
     1981 atagcgggca tctggaaatg atggaaattg ccaggcgcag tctggaagcg cggggccgca
     2041 ccgtaatagg cggctacctc tccccatccc atgccaccta tgtcgaccca aaatgcggca
     2101 gcgaaacgct ctcctcctcg caccgcctca tgctctgcca ggaagccgta aaggatagcg
     2161 actggctcat ggcctgcggc tgggaagcac tgggagtgga gcgcatgctc aacttcaccg
     2221 atgtgattat caggctggaa cgttatctgg agaaccaact tgccaaatca gacctggcaa
     2281 gacgtgcaga taagagccga accaaccgaa caacctatgg tccggcaccg gttgtttact
     2341 atgtcttcgg cagcgacaat gcacgcttca cccgcggttt tctccctgac ggtaaatgtg
     2401 tctgcgtgct gcgccccggc tcggaggaac cctaccacga atttgcttgc gcccctctgg
     2461 tacaagcaaa cagcgagata attttcgccg gggaagccag ccatgatctc tcctcccagt
     2521 ccatccgccg ccgccgctcc gcccagactt ccttccacag tgcaccggcc agctattttc
     2581 actggcaaac acagagcgaa ggagaccaac cagctgccac catctatttg cgtgatgaag
     2641 gactctggtc cctatcttct tttcccttac cggaagaaac actgagtacc gctctcgaca
     2701 aattcaaaga agggcttcta gccttgatga ttgaatgcca tgaaagagca agttggcccg
     2761 ataccaaaag gcaactgcag gtggagctac tttcactttc ggagcagcgc caggccgctc
     2821 agaagctggc tcgcgggcac agcatcatca gcctggatcc ctgtattgaa ggcgacttca
     2881 atctggaaat ctcaagatgc tatcccctca atgagcccct cgccacaccg accatggtgg
     2941 cacgcccggg ggccgcttcc atcgtcaagc aagtggggct gattccgccc ggtcaataca
     3001 ttctcttcga cgacgacatc ttcagcggct ccaccgtgcg tcaggtacaa gaccttctgc
     3061 ctgactccat agccattaga gcagtctgcg ccctcactat caggggcacc agtaaacaca
     3121 ccgtgctcga catcctcgac ttgcgcgact tcctggccgg cgccaggctg gccgggcttg
     3181 tgatgagcac
//
LOCUS       DAOQNZ010000312         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15340_length_3189_cov_3.881940, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000312 DAOQNZ010000000
VERSION     DAOQNZ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15340_length_3189_cov_3.881940"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(981..>3189)
                     /locus_tag="PKW73_06860"
     CDS             complement(981..>3189)
                     /locus_tag="PKW73_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73039.1"
                     /translation="VAGVDGKAHGYSQTLDLMELGLDPRKVSSDDAARLLASRTGKGS
                     EPRNIIGNKDHFKGEEGDHVPGSKPEPGDAAKVPDATKPGDVPVVVEAAPAVGKYVTK
                     AEQELNVNTVSKLAEMNKQDMAGWTDEAVMVQDALGQMIGPMHAATTPSYKPLDPGYI
                     AVRNAMVQIGNQVKGQADLQHVYPLFNRAWMAANQHIGMGLSEVNGWTHEFNLFGREI
                     HSSLMANMKRHGIDTDTVLRSKNPPLFSISADGGAGPHTMRQIDGVWPLDHVLYPRNM
                     VGTRSVTASGIYGHEIGHDQYGGILKFDESIREQVIKDAVKNGLLALEKKLYGTETGK
                     IAAEKVAVPGHGEMTKADLYENIFKAQADENTADIWGAAWTGHNGGGALGILLQSLRS
                     GGKLETRNVYGKEFVDAVENPLGFEVHAFDALRPKIVAETMRARANGDKRVLDHADAL
                     ERYGHEASRPGDYVFANIDAPTDRIVIPRQELEAVVPELIKAQMNTPLPALQGKTFGD
                     VLPDLPSHMAKMDTLAELMADAVLKGKKPSEIPFDTNQYTINQVFGAGMPAALKLVAG
                     GMDAVKANAEVNRMSDFLRSQFHANDPHISPLRPTSLQAIRLNSPRSFVEGGRILGSE
                     AKHAASRILRANPAIRDYIGQRAPIFGGAEAAITAERLMGDNDKTKETATVMDMTPKP
                     PFAPMQATGKTDWLSGKDSTLRNILFMQKASRDMITTAQAEAATPQPENLTGK"
BASE COUNT          665 a          822 c          899 g          803 t
ORIGIN      
        1 ctttctgacg caacagttgg cctcgggtac ggatttaatt tgacgcaata cgttccgtcg
       61 aaaatcgctc acttatgaaa attcgggctt aaatccaacc aaaggcaagc taatgagcga
      121 aattatggat aatttcactc acgccccaat ttctgaaaag acaggggtgg gtcttgccta
      181 accggaatat aattgcgcgc cctattctgc ataagattcc aggtagccgc agccagtgca
      241 gcgataggtt gatgccagtc ccattttgtc cttgtgaacg tccggccatt cacccacttt
      301 catgcccaga acgcgaacct cggactcctt gggggcggtg tgacaattgg aattggctta
      361 caggcagtgc tcacgaactt tcttcgaaaa gagggaagca aaccattccc agaagatcgg
      421 gacagttcga catgcaagct tctaagcaaa agactgcgtt gggtctggtt gaagcccgac
      481 ctccaaatgg aatgagtgtg gcatgcaagt aacagggagt ctcttctttc gccgaaagta
      541 gtatcgaccg ggccctgttt tccaaactgg agatggagcc atagctgcct tctataaatc
      601 gaagctgacg caggacttca gtcgttattg caggcaaagt aatcggatct ttctggctcc
      661 tgaagataaa cctgaaaaaa gcgtgtgact caaaatactc cctggaagca cgttcaagga
      721 agtcgtcctt ttcaaggtct ataccattgt cgtgacagag actaagcact tgagaaagga
      781 cgttgtcgta gacctttgat tcaatagtct gatggcacaa ccacggaagc tgctggtagc
      841 gaacattggc cagcgcatgt gtgatagcac cgtcggaagg agatagcatt cgttaacctt
      901 gatttcggct tgaacaaccg cgagacgccc atttagtatg aagcgtcatt ttaaggctaa
      961 ccgacaatcc atttactttc ttattttcca gtcaaattct ccggttgagg tgttgcagcc
     1021 tccgcttgag cggtggtgat catgtcacgg ctggcttttt gcatgaagag aatgttccgt
     1081 aaggtactgt ctttaccgga aagccagtcg gtcttgcccg tagcttgcat gggggcaaag
     1141 ggcggcttgg gagtcatatc cataactgtg gcagtttctt tcgtcttgtc attgtcgccc
     1201 atgagacgct cggcggttat agcagcttcc gcaccgccga agatcggcgc tctctgtccg
     1261 atatagtcgc gtatagcggg attggctctg agaatgcggc tggccgcgtg cttggcttcg
     1321 ctgcccagga tacgaccacc ttccacgaag gagcgagggg agttgagtct gatcgcttgc
     1381 agggaagttg gtctcaaggg tgaaatgtga gggtcgttgg cgtggaattg cgagcgcagg
     1441 aagtcggaca tgcggttcac ttccgcattg gccttgaccg catccatgcc acctgccacc
     1501 agtttgaggg cagcgggcat gccggcgccg aaaacctggt tgatggtgta ctgattggtg
     1561 tcgaaaggaa tctcgctcgg tttcttgcct ttcagtacgg catcggccat aagttctgcc
     1621 agagtgtcca ttttggccat gtggctgggc agatccggca gcacatcacc gaaggttttg
     1681 ccctgcaagg ccggcagagg tgtattcatc tgggccttga tcagttcggg cacaaccgct
     1741 tccagttcct ggcggggaat gacgatgcgg tcggtgggcg catcaatgtt agcgaaaaca
     1801 taatcgcccg gtctggaagc ttcgtgacca tatctttcca gagcatcggc atgatcaagc
     1861 acgcgcttgt cgccattggc cctggcccgc atagtttcag ctactatctt cggtctcaag
     1921 gcatcgaagg cgtgtacctc gaaaccaagt gggttctcca cggcgtcgac gaattcctta
     1981 ccgtatacgt tacgggtctc gagcttgccg cctgatctca gtgactgcaa gagtatgccg
     2041 agcgctccgc cgccgttgtg accggtccag gctgcgcccc agatatcggc cgtgttttcg
     2101 tcggcttgtg ctttaaagat gttctcgtag aggtcggctt tggtcatttc accatgaccg
     2161 ggaactgcta ccttctcggc ggcaattttg ccggtttcag tgccgtagag cttcttctca
     2221 agggccaaga gaccattctt gacggcgtct ttgatcacct gttcgcgaat tgactcatcg
     2281 aacttgagga taccgccgta ctggtcgtgt ccgatttcat gaccgtagat accgctggcg
     2341 gtgacacttc tggttcccac catgttgcgc gggtagagca cgtggtccag gggccagaca
     2401 ccatcgatct gacgcatggt gtgcggtccg gcgccgccgt cggcggagat ggagaagagt
     2461 ggtgggttct tggagcgcag aacggtgtcc gtgtcgatgc catggcgctt catgttggcc
     2521 atgagagagg aatgaatctc tcgaccgaag agattgaatt cgtgggtcca gccgtttact
     2581 tccgaaagac ccatgcctat atgctggttg gcggccatcc aggcccggtt gaagagtggg
     2641 tagacgtgtt gcaggtcagc ctgtcctttg acctgattgc cgatctgaac catggcgttg
     2701 cgcacggcta tgtagccagg atcaagcggc ttgtagctgg gggtggtagc tgcatgcatg
     2761 ggaccaatca tctgaccgag agcatcctgc accataaccg cttcatcggt ccaaccggcc
     2821 atgtcctgct tgttcatctc ggccagtttg ctgacggtgt taacgttcag ctcttgctct
     2881 gccttggtga cgtatttccc tacggctggt gcagcttcca ctactaccgg cacatcgccc
     2941 ggttttgttg catcaggaac cttggcggca tcgccgggct ccggtttgga gccgggcaca
     3001 tgatcgcctt cctcaccttt gaaatggtct ttgttgccga ttatgtttct tggttcgctg
     3061 cccttgccgg tgcgactggc gagcagtctg gcggcatcgt cggaggagac cttgcgggga
     3121 tccagaccca gttccatcaa atcaagagtc tgggagtaac cgtgggcttt gccgtctact
     3181 ccggctact
//
LOCUS       DAOQNZ010000313         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15368_length_3186_cov_3.486745, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000313 DAOQNZ010000000
VERSION     DAOQNZ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15368_length_3186_cov_3.486745"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2758)
                     /gene="dnaE"
                     /locus_tag="PKW73_06865"
     CDS             complement(<1..2758)
                     /gene="dnaE"
                     /locus_tag="PKW73_06865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNA73040.1"
                     /translation="MSPRPYVPLHLHTEYSLLDGATMIKDLVKKAKAQNMPAVAITDH
                     GVMYGAIELTKKCHEVGGIKPIIGCEAYIIDGDFTDKTTKQPLYHLTMIARNKEGYKN
                     LVRLNSRAHIQGYYYKPRINKELLAAHSEGLIVLSGCLGAELSQYLLKDNYEKARDAA
                     AWYKDVFKENYYIEIQDHGYPEDRRVNKHLITIARELNIKLCATNDSHFTNKADAKAH
                     DCLLCIQMGKNVTEPNRMKFSGWEYIKNGDEMACLFRDHLDLEYIEESIYSTLEIADK
                     VEPVKLASDPRLPVFEVPQGHTAESFLHHLVMEGLKQKFGGIDKPVEDRAFRELKVIE
                     DMNFASYFLIVSDFINYAREKGIPVGPGRGSAAGSIVAYALGITNIDPLKYNLLFERF
                     LNPERKSMPDIDTDFCIERRGEVIKYCAEKYGEDRVAQIITFNRMTSKAVIKDVARVL
                     EFPFGEAGKLAKMVPVVRGKPTPLDEMVEDHPEFKKVYQTSDEARQVIDLARKLEGVN
                     KTFGMHAAGVVISDVPLEEIVPVQRNNDGTVICQYYMEDVAYAGLVKMDFLGLRNLTM
                     IDKAVKILKKTRGIEIDVDLIPMDDEKTYQTISNGDLAGIFQLETSSGMKQVARDMRP
                     SNMEDISALIALYRPGPLDTGMIDKFIDCKHGKMKITYLTPLLEGILKDTYGQIVYQE
                     QVMQIAQVLGGYSLGQADLLRRAMGKKKPEEMEKQRELFVGGCAKNAVSKEIADQLFD
                     MMVQFAEYCFNKSHSAAYGVLTYQTAYLKTHYPVEYMTALLSSVTGDQEKLQGYIAEC
                     QSMNIEILPPDVNGSGSDFTASGNTIRFGLSAIKGLGEGAVQTIVNEREKGGNFETIQ
                     SFLSRLDLRVVNKKALESLIRSGALLSTGVTRKQALTNLDSLVESALRKQNQEATGQI
                     SLFSL"
     gene            complement(2990..>3186)
                     /locus_tag="PKW73_06870"
     CDS             complement(2990..>3186)
                     /locus_tag="PKW73_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73041.1"
                     /translation="LSADERRCLALWFRQAIAKLELEEVTQVMLKEFPPEGETSAHRS
                     AVARRRATRGRVSTQAKQLV"
BASE COUNT          726 a          813 c          818 g          829 t
ORIGIN      
        1 ccaggctgaa gagactaatc tgcccggtgg cctcctgatt ttgcttgcgc agggcacttt
       61 ccaccaggga gtcgaggttg gtgagggcct gcttgcgcgt cacccccgta gacaaaaggg
      121 caccggaacg aatcagcgat tcaagagcct ttttattgac cactcgcagg tctaggcgag
      181 agaggaaact ctgaattgtt tcaaagttgc cgcccttctc acgctcattg acaatggtct
      241 gcacggctcc ttcacccaga cctttgatgg cggaaagccc gaatcgaata gtattgccgc
      301 tggctgtaaa gtcgctaccg gagccgttta catcaggagg cagaatttca atgttcatgc
      361 tctgacattc ggcgatatag ccctgtaatt tttcttgatc accggtaact gacgacaaca
      421 atgccgtcat gtattcaacg ggataatgag tttttagata ggccgtttga tatgtgagca
      481 caccataggc ggcactatgg gatttgttga aacagtactc ggcaaactgc accatcatgt
      541 cgaaaagctg gtcggcaatc tctttggaga ctgcattttt ggcgcagcct cccacaaaga
      601 gttccctctg cttttccatc tcttcgggct ttttcttacc catagcccgg cgcaacaggt
      661 cggcttgccc gaggctgtag ccgcccagca cctgggctat ctgcataacc tgctcttggt
      721 agactatctg cccgtaggta tctttgagga tgccttccag aagcggcgtc agataggtaa
      781 tcttcatttt gccgtgctta caatcgataa acttatcgat catgcccgta tcaagcggac
      841 ccggacgata aagagcaatg agagcggaaa tgtcttccat gttggatgga cgcatatcgc
      901 gggccacctg cttcatgccg gaagacgttt caagctggaa gatgccggcc aggtcgccgt
      961 tggaaatcgt ctgataggtc ttctcgtcgt ccatcgggat gagatcgacg tctatttcga
     1021 tgcctctggt tttcttgaga attttgacgg ccttatcgat catggtcagg ttgcgcagac
     1081 ctagaaagtc catcttaacc aggccggcat aggcaacgtc ttccatgtag tactggcaga
     1141 tgacggtgcc gtcattgttt ctttgcaccg gcacaatttc ttctaagggc acatcggaaa
     1201 taaccacgcc ggcagcgtgc atgccaaagg ttttgttgac cccttccagt ttgcgagcca
     1261 gatcaataac ctgcctggct tcatcggagg tctgatagac cttcttgaac tcggggtggt
     1321 cttccaccat ttcgtcgagg ggagtaggtt tgccccgcac taccggcacc attttggcaa
     1381 gcttacccgc ctccccaaag gggaactcaa gcacccttgc cacatctttg atgacggcct
     1441 tactggtcat gcggttgaaa gtaatgatct gcgccactct gtcttcgcca tacttctcgg
     1501 cgcagtattt gatcacctcg ccgcgcctct cgatgcagaa gtcggtatcg atatcgggca
     1561 tggacttacg ctcggggttc aagaagcgct caaagagtaa gttgtacttg agaggatcga
     1621 tgtttgtaat accaagagca taggcaacta tggaaccagc ggcggagcca cgccccggcc
     1681 ctacgggaat gcccttttcg cgagcataat tgatgaagtc ggaaacgata agaaagtaag
     1741 aagcgaagtt catgtcttcg attactttca actcgcgaaa ggctctgtct tcaaccggtt
     1801 tatcaatgcc gccgaacttc tgcttgagcc cttccataac cagatggtgc aaaaagctct
     1861 cagccgtatg cccctgcggc acttcaaaca caggcagacg ggggtcgctg gccagtttga
     1921 ccggctctac cttgtcggca atttcaaggg tgctgtatat cgactcttcg atgtattcga
     1981 gatcaaggtg atcgcgaaac aagcaagcca tctcgtcgcc gtttttgatg tattcccagc
     2041 cgctgaactt catgcgattg ggttcggtga cgtttttgcc catctggatg caaagcaagc
     2101 aatcatgcgc tttggcatcg gccttattgg taaagtggct gtcgttggtg gcacaaagtt
     2161 tgatattcag ttcgcgggca atcgtgatga gatgcttatt gacgcgccgg tcttccggat
     2221 agccgtggtc ttgaatttct atgtaatagt tttctttgaa gacgtctttg taccaggcag
     2281 cagcatcacg tgccttttcg taattgtctt tgagaagata ttgagaaagc tcggctccca
     2341 ggcagcccga aagaacaatc agcccctcac tatgggcggc taaaagctct ttgttgatac
     2401 gcggtttgta gtaatagccc tgaatgtggg cccgcgaatt gagacggacc aggtttttat
     2461 agccctcttt attgcgggcg atcattgtca ggtgatagag gggctgtttg gtggtcttgt
     2521 cggtaaaatc accatcaata atgtaggcct cgcagccgat aatgggcttg atacctccca
     2581 cttcatggca tttcttggtt aattcgatgg ctccgtacat gacgccgtgg tcggtgatag
     2641 ccacagccgg catgttctga gccttggcct tcttgaccag gtctttgatc atggtggcac
     2701 catcgagcag gctgtactcg gtatgcaaat gtagcggaac atatggacgc ggactcatga
     2761 tcaccccgaa tgccaaaaga atgaggactt attatctgca ctaaagagat aattccccct
     2821 agatctataa ctgtaatctc tttactgcca agacttaacc ccatctctat agagctgact
     2881 aatagagaag acggctgttt gccttagaaa gttcaatttt tgccagaaaa aaagtggacc
     2941 ccccggggga gcccacctta atgaattttc gaaccgcctc gcctgaggac tagaccagtt
     3001 gcttggcctg ggtggaaact ctgccgcggg tagcgcggcg acgagccaca gcgcttctgt
     3061 gagccgacgt ttcgccttcg ggcgggaact ctttgagcat tacctgggtg acttcttcaa
     3121 gttccagttt agctatggct tgtctgaacc acaaggccag gcaacgtctc tcgtcggcag
     3181 acaggg
//
LOCUS       DAOQNZ010000314         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15371_length_3186_cov_2.972533, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000314 DAOQNZ010000000
VERSION     DAOQNZ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15371_length_3186_cov_2.972533"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKW73_06875"
     CDS             complement(<1..626)
                     /locus_tag="PKW73_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNA73042.1"
                     /translation="MRVLRAAVVGAGAMGSGIACVLSQCGIEVLLKDVEQQAVDRGLG
                     NIKRMYDSRVKKGVNTQEEADKLLALVKGTTSYDDFKNVDLVIEAAFEEISVKLDIFK
                     TLDEICPKKAILATNTSALSVSEIAAATSRPEKVLGMHFFNPAQFMKLVEVIPGVHTS
                     EETVKTAMDLCQEMGKTPVKVEECPGFLVNRVLFTYMNEALYMLQEGTA"
     gene            717..2132
                     /locus_tag="PKW73_06880"
     CDS             717..2132
                     /locus_tag="PKW73_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNA73043.1"
                     /translation="MSLLTTLMRRTTDADFGAAADTPKHLGWLMLTALGIGATIGAGI
                     FAMPGIIAGKAGPAGILSFVITGIVIMIVAICYEKFSVKVPHGVSAYSYVYYSIGEVM
                     AWIVAFGLFLEYSFGASAVSIAWGEYLKNATGLKIDPFWCGPVIDHAGQFHFGVNIIA
                     IGVIFLVSTILLLGGVSKSAKLNFFLVLLKMTLLVMFLGFGIRHVNPQNWFPFMPHGF
                     EGVLKGAATAVFPYVGFDALYTFARESKSLKDTRMATYWCVGIVAFLYISVMAVATGL
                     APCTINGHPNELFVGTEAAAPLAKLLASVGEGWTSVFISFGAVLGIFNVLLVFCMGGP
                     RIFKNMAEDGLLPPVFQKTSKGNPVLGIILNGIIVAAIAGFVPFGSIADMMVLGTLVA
                     FVLVCLGALRLKLVHPIIAILGAVGCSILACNLEPMVLKVYSITLPVGLLIYFFYGFK
                     NSKLGKSRAAARASEAEPVNS"
     gene            2239..2802
                     /locus_tag="PKW73_06885"
     CDS             2239..2802
                     /locus_tag="PKW73_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73044.1"
                     /translation="MHANGQELNSGRRLADKLYRRLVFLRRDLQMHPAIVESDLFREY
                     LACAAPWLRASYCLMDLIERVKNFGLTGFFLDTGWWQQYETTEALALVGAEVREQFEL
                     SRQSFDAGLDPDVGLDKERFCQSILEALCLHLSSESEAAYDWLVLMNRYGALGSPADS
                     GTAISPDVNVVMARGSAISPYGVQVFS"
     gene            complement(2814..>3186)
                     /locus_tag="PKW73_06890"
     CDS             complement(2814..>3186)
                     /locus_tag="PKW73_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNA73045.1"
                     /translation="IEEAREHDYAKTIWGRKRYFTHLHDRNDNVRKADERAACNAPIQ
                     GSAADLVKLAMIKLDQELSKSGLQAKLIMQVHDELILEVPDQELDATKALLGKAMQLD
                     QPLKVPLKVDFGVAKNWMDAK"
BASE COUNT          604 a          814 c          885 g          883 t
ORIGIN      
        1 gccgtgcctt cctgcagcat gtagagggct tcgttcatat aagtgaagag cacgcggttg
       61 accaggaagc ccgggcattc ttcaaccttg acgggagttt tgcccatttc ctggcagagg
      121 tccatagccg tcttgaccgt ctcttcagag gtatgtacgc cggggatgac ctcgaccagc
      181 ttcatgaact gggccgggtt gaaaaagtgc atgcccagca ctttctcggg acgactggtg
      241 gcggcggcta tttccgagac ggaaagagca gaggtattgg tggccaggat ggcttttttg
      301 gggcagattt cgtcaagggt cttaaatatg tccagcttga ctgaaatttc ttcgaaggcc
      361 gcttcgatga ccaggtctac gttcttaaaa tcatcataag aagtggtccc cttcaccagg
      421 gccaggagct tgtcagcctc ttcctgggta ttgacgccct ttttgacacg ggagtcgtac
      481 attcttttga tattgccaag accacggtct acagcttgct gctcgacatc tttgagcaaa
      541 acttcgatac cgcactggct gagaacgcat gcaatgccgc tgcccatggc tccggcgcct
      601 acaactgctg ctctgagtac tctcatttaa aagcctcact gctataaaag aaggaaattt
      661 ggcgctatgc tagcattttt gtttcgcagg ctcgccaaat cgtaaggaat tagtatatgt
      721 cgctgttgac aacgctgatg cgccgtacca cagatgctga tttcggtgcc gccgccgata
      781 cgccgaagca cctgggttgg ctcatgctca cggcccttgg cattggtgcc accatcggtg
      841 ccggtatctt cgccatgccc ggcattatcg ccggcaaagc agggccggcg ggcattctat
      901 ccttcgtgat taccggcatt gtgattatga tcgtggccat ctgctacgag aaattctcgg
      961 tcaaggtgcc ccatggtgtt tccgcctaca gctatgttta ttattccatc ggcgaagtga
     1021 tggcctggat cgtggctttc ggtctctttc ttgaatacag ctttggtgct tccgccgtct
     1081 ccattgcctg gggcgaatac ctgaagaatg ccacgggctt gaaaattgat cccttctggt
     1141 gtggacccgt aatcgatcat gccgggcagt ttcacttcgg ggtcaatatc attgccatcg
     1201 gcgttatctt cctggtttct acaattttgc ttttaggggg cgtcagcaag tccgccaagc
     1261 tcaatttctt cctggtgctg ttgaaaatga ctttgctggt catgtttctt gggtttggta
     1321 tcaggcatgt caatccgcag aactggttcc ctttcatgcc tcatggtttc gaaggcgtgt
     1381 tgaaaggggc tgctacggca gttttccctt atgtagggtt tgatgcgctc tatacttttg
     1441 cccgcgaatc aaagagtctt aaagacacga gaatggccac ctactggtgc gtgggcatag
     1501 tggctttcct atatatatca gtcatggccg tagcaaccgg tcttgccccc tgcaccataa
     1561 acgggcatcc caacgaactc tttgtgggaa cggaagcggc tgcgcccctg gctaagcttc
     1621 tggccagcgt gggcgagggt tggacttcgg tcttcatctc ttttggtgcc gtgcttggaa
     1681 ttttcaacgt gcttctggtc ttctgtatgg gtgggcctcg tatcttcaag aatatggccg
     1741 aagacggtct ccttcctcct gtctttcaga agacttccaa gggtaatccg gtgctgggca
     1801 ttatcttgaa cggtattatc gtggctgcca ttgccggctt tgtgcccttc ggcagtattg
     1861 ccgacatgat ggtgctgggt accctggtgg cctttgtgct ggtctgcctc ggtgcactcc
     1921 gcttgaagct ggttcatccc attatcgcca ttttgggcgc ggtgggttgt tccattctgg
     1981 cttgcaatct ggagcccatg gtgctcaagg tatacagtat taccctgccg gttggtcttt
     2041 tgatctactt cttctacggc ttcaagaata gcaaactggg caaatcccgt gctgccgcca
     2101 gggcttccga ggcagaaccg gtcaattcct gagagtgcca gactttcaga cctcctcgta
     2161 tttcccgcaa tacaatgcca cgctttagtc acgtctggcg ccgcataatg gcgctggtca
     2221 atatggcggg aatattttat gcatgcaaac gggcaagagt tgaattccgg taggcgtctg
     2281 gcagacaagt tgtatcgacg gctggtcttt ttaagacggg atttgcaaat gcacccggcc
     2341 attgtggaga gcgatctatt ccgggagtat ctggcctgtg cggcgccctg gttgcgggcc
     2401 agctattgcc tgatggattt gattgagcgg gtaaaaaact tcggtctgac cggcttcttt
     2461 ctcgataccg gttggtggca gcaatatgag actaccgagg ctctagccct ggtgggcgcc
     2521 gaggtgagag agcagtttga gctttccagg cagagcttcg acgcggggct tgatcctgat
     2581 gtcggtcttg ataaagagcg cttctgccag tcgatactgg aggccctgtg cctgcatttg
     2641 agcagtgaga gcgaagccgc ctatgactgg ctggtgctta tgaatcgata tggtgccctg
     2701 ggctcgcctg ccgactcggg tacggcgatc agccctgatg taaatgtggt tatggctaga
     2761 ggcagtgcca tcagccccta tggcgtgcag gtgtttagct gaatgggcag ccgctattta
     2821 gcgtccatcc agtttttcgc cacgccgaag tcgactttga gcggcacctt gaggggttga
     2881 tccaactgca tggctttgcc cagcagagct ttggttgcat ccagctcttg atccggcact
     2941 tccagaatca gttcatcgtg cacctgcatg atcaatttgg cctggagacc ggatttgctc
     3001 agttcctggt ctaacttgat catggccagt ttgaccaggt cggcggcgct gccctggatg
     3061 ggcgcattac aggctgctct ttcatcggct ttgcgtacgt tgtcgtttct atcgtgcaga
     3121 tgggtgaaat aacgcttgcg accccatatg gtcttggcgt agtcgtgctc ccttgcttct
     3181 tcgatg
//
LOCUS       DAOQNZ010000315         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15380_length_3185_cov_3.512460, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000315 DAOQNZ010000000
VERSION     DAOQNZ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15380_length_3185_cov_3.512460"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..1244)
                     /locus_tag="PKW73_06895"
     CDS             complement(276..1244)
                     /locus_tag="PKW73_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73046.1"
                     /translation="MAASRRLIPKVIGSVIALGALLSTAVANAPGALAARGDRLDRDT
                     SALVDAVRASLTAQGQWPPANGSVQMNLCQALEVFSRTVKESPDLSRSSNFSGNSEVY
                     MAAQRLQAAAAAVDPLIGLASSGNVGGLWLQIKNEISAQNSFAAVASGMGGGSMFMPG
                     AYYPNQAFAPSINGFPAGGTFPGAINTNSTYSPYASQFGNGYYGGVNPASLQDAANRY
                     NGELNSFINEAQKNLGRGGFQTADPGLIMNMSTALSFLQQTSRSVTRALSNNGNFAAR
                     QAYIGQILSASDRFESAFKAASPSIPLQMRFASVKQQLTFLVQLSR"
     gene            complement(1391..1738)
                     /locus_tag="PKW73_06900"
     CDS             complement(1391..1738)
                     /locus_tag="PKW73_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73047.1"
                     /translation="MSDQLEAIRKQADAGNGCEVADALHAMNTQTLHEEFQKLSKLDG
                     AGSSSSVFSENKLVSGRTQFIVFDTLQLSGRFDTKDQHIIFERLVGSDGGKMSEVCRS
                     PGPQAPFTNLDQY"
     gene            complement(1942..>3185)
                     /locus_tag="PKW73_06905"
     CDS             complement(1942..>3185)
                     /locus_tag="PKW73_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73048.1"
                     /translation="DTTIVGQGAEVKTAGTDQKSAGADGEAAKDKVQPAADATGNVLP
                     PVDIYDDKRNNPDGGDKGAAPKSGDAAPKDPELNDEQRKALDEHMTKYKQELDSKNFT
                     LDPIQKGWGPYQSLESMVNSGKIQMTRAELKEESYRIRDRDFADMGRYYYKVGEAPVR
                     WNEAELTKKMETERAEVKQQMIKAEEERKAKEAEEAKRKEEEARVQAEQVAKAVDSNV
                     PKVEIIAEAQKAAGMAGDPGQLRDRIKEHVTKEVNAGTLTPEDLAKPMPRVGAIYVGS
                     GVLTGEELQAGLAEQAKRKEEAAKTGAEGPKLGDVLKDMHKDNPEKVAGIDQASKLYE
                     SMKVQAEKQRADDLAKAEADAKAKADADAKAKTEADAKAKAEADAKAKAEADAKAKTG
                     KQPDPVPVPQQKIEQEPRKPI"
BASE COUNT          674 a          935 c          790 g          786 t
ORIGIN      
        1 ccccgtgaac gccgccgaaa acacagtcgc cgacaatgaa cgtgagcgaa tctgcactcg
       61 aggacagaaa agaatccgac ttccatccgg ctccggcagt tcaggagcaa gcagcatccc
      121 actagtcgcg cttaacttga gggcccgaac cacttccctg gagattcctg caccatcaac
      181 cgctctacgg caagaaccgc aacctcaaac gttactcttg tcgggagaca ttatcttctg
      241 caaaatcctc tccaatcaac ggtcaagacc tcattctaac ggctcagctg caccaggaat
      301 gtgagctgct gcttgaccga agcaaagcgc atctgcagag gaatggaggg agaggccgct
      361 ttgaaagccg actcaaaccg atcagaggca ctcagtatct gccctatata agcttgccgg
      421 gcggcgaagt taccgttatt actcagggct ctagtgacgg aacgacttgt ctgttgtaag
      481 aaactcaagg ccgtactcat attcataatc agtcccggat cggcagtttg aaaaccgccc
      541 cgccccaaat tcttctgagc ttcgttgata aagctgttca gttcgccgtt gtatctattg
      601 gcggcatcct gaagcgaggc cggattgacg ccgccgtaat agccattgcc aaactggctt
      661 gcgtagggac tgtaagtact attggtattg atagcacccg ggaaagtacc accggcagga
      721 aacccgttga tggatggggc aaaagcctga ttggggtaat aggcgccagg cataaacata
      781 ctaccgccgc ccatgccgct ggcgacagcg gcgaagctat tctgggcgct aatctcgttc
      841 ttgatttgaa gccagagccc tccgacatta ccactggagg caagacctat caacggatct
      901 acagcggcag ccgccgcttg cagacgctgt gccgccatgt acacttcgct atttccggaa
      961 aaattgctgg agcggctcaa atcaggactt tctttcacgg tgcgggagaa aacttccagc
     1021 gcctgacaaa gattcatttg cacagagcca ttggcagggg gccactgacc ctgggcagtc
     1081 agactggccc gcaccgcatc taccaaggcc gaagtgtcgc ggtcaaggcg gtcgccacga
     1141 gcggctaagg cacctggtgc gttcgccact gccgtggaca gtagagcacc gagcgctata
     1201 acggaaccaa taacttttgg tatcaaccgg cgagaggctg ccataatcca ttcctaacct
     1261 gatgagtgct tattccggcg ccaaccttta accagcggat gtttaataga ttgagcctaa
     1321 aagagtttag cacctcaggt atgcaagcct aaaaggcctt gcccaagcag acaccgcgac
     1381 aggtcggaaa tcaatattgg tccagatttg tgaatggcgc ctgtggtccg ggcgagcgac
     1441 agacttcgga catcttaccg ccatcgcttc ccacaagccg ttcaaaaatt atgtgctggt
     1501 ccttggtgtc aaagcgaccg gagagttgta acgtatcaaa aacaataaac tgggttctgc
     1561 cgctaacaag cttgttttca ctaaaaacac tagacgagga gcctgctcca tccagtttgc
     1621 tcaacttctg gaattcctcg tgcagggtct gagtattcat ggcatgcaac gcatccgcca
     1681 cctcgcaccc attgccggca tccgcctgtt ttcgaatagc ctcaagttga tcggacatgg
     1741 tgcatacctc gtggttctac aagctaaatt ctatctattg cctgtgtcct agcagtgtca
     1801 ggaagagcgg aaaacacgtg tccatctcca ggcaccagat gcagattcgg ctccagtacc
     1861 tcaatgcacc agctcatccg actcgcaaca aaaagccggc actgaatgta gtgccggctc
     1921 ttcatcaaac tgttatggaa cttatatcgg cttgcggggc tcctgctcaa tcttctgctg
     1981 tggcacgggc accggatccg gctgcttacc agtcttggct ttagcatcgg cttccgcctt
     2041 ggctttagca tcggcttccg ccttcgcttt agcatcggct tccgtcttcg ctttagcgtc
     2101 ggcatccgcc ttcgctttag cgtcggcttc cgccttggcc aggtcatcgg ctctttgctt
     2161 ttccgcctga actttcatgc tctcgtagag cttgctcgcc tggtcaatgc cggccacctt
     2221 ctcggggtta tctttgtgca tatccttgag cacatcccct agtttgggac cttcagctcc
     2281 ggtcttggcg gcctcttcct tgcgtttagc ctgttcggca agtccggcct gcaactcctc
     2341 accggtcagc acgccggaac cgacatagat ggctcccact cgcggcatcg gcttggccag
     2401 gtcctcggga gtgagagtgc cggcattaac ttccttggtt acatgctctt taatacgatc
     2461 tctcaactgt ccggggtcgc cggccatgcc ggcagccttc tgagcttcgg caataatttc
     2521 gaccttaggc acattactgt cgacggcctt agccacttgc tcggcctgca cccgggcttc
     2581 ttcttcctta cgtttagctt cttccgcttc cttagctttg cgctcctctt ccgccttaat
     2641 catctgttgt ttgacttcag cgcgctcggt ctccatcttc ttagtcaatt ccgcttcatt
     2701 ccaacgaaca ggtgcctcgc ccactttgta gtagtagcgg cccatgtcgg cgaagtcgcg
     2761 gtcgcgaata cgataggact cttctttcag ctcggccctg gtcatctgaa ttttgcccga
     2821 gttcaccatc gactcaagag actgataggg tccccagcct ttctgaatgg gatcaagggt
     2881 gaaattcttg gagtcgagtt cttgtttgta cttggtcatg tgctcatcaa gagccttgcg
     2941 ctgctcgtcg ttgagctccg ggtctttggg agcagcatca ccactcttgg gagcagcgcc
     3001 tttatccccg ccatcagggt tattgcgttt atcgtcatat atgtccaccg gcggcaacac
     3061 attaccagtg gcatccgcgg ccggttgaac cttatcttta gccgcttcgc cgtcggctcc
     3121 ggccgacttc tggtctgtgc cggcagtttt cacttccgct ccctgaccta ctattgtagt
     3181 gtcgg
//
LOCUS       DAOQNZ010000316         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15386_length_3184_cov_4.631831, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000316 DAOQNZ010000000
VERSION     DAOQNZ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15386_length_3184_cov_4.631831"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..981)
                     /locus_tag="PKW73_06910"
     CDS             complement(202..981)
                     /locus_tag="PKW73_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73049.1"
                     /translation="MQTNIPKVTLAANSLEKAVSSWLAVILGLVVPLVTLLVCTGIKG
                     TITTDTMLISANLHMVVLALIPLIAIRSGVDFQLRLNAWRHEALMGTIFWLSLTVQAI
                     SWMTASLNNADKMELFVSKFSSQQCSIGVPCPVQGGVVNIPYVASLMRAQETLRNEIG
                     TLNKILSYRTVAAETRPNNLEQLDGFIEPMNQLGKLKDSLQALTSPDYALFKAVVEEG
                     GAVCFERLEQCPALDLSQYKRKLTGQNPLSSAVVRAFGLTD"
     gene            complement(1006..1578)
                     /locus_tag="PKW73_06915"
     CDS             complement(1006..1578)
                     /locus_tag="PKW73_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73050.1"
                     /translation="MNTEAICTQPESLMQFLGLYGPAGLIMLLGLAVIAALFRPALEW
                     LEEALSYLLWQLCGAPVHENMSSAILARCKTRIANLLGDRAMMSDSGLNKAEPYFEQY
                     SQFVDQMTELLKFGTSLHWSGAYKLSFDNSRNILKLVDAFEQVANSRASSSAQEAWLK
                     FNLDALKTITDRSAALNKDLLSIMADGRLV"
     gene            2689..>3184
                     /locus_tag="PKW73_06920"
     CDS             2689..>3184
                     /locus_tag="PKW73_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA73051.1"
                     /translation="MSAQIATSVSGRRRRLNPKVLPDAVIGFKEHLDANLFGQQEGKD
                     LLTFAFTAAHNPLRNRNKPIAFIINAGKSRTGKTHSVELLAERIHGNPKAYLRIDMAE
                     YMDKYSISNLKGSGRGYINNQSARDEGYNAVAKDVKHDYAEFTNHNLEWSKRGSTVPV
                     SIVLL"
BASE COUNT          698 a          839 c          846 g          801 t
ORIGIN      
        1 cggttcttct ttctccattc tgccctgccg ctcagatttt ccaggccatt tctttgaatt
       61 gatagatact atatcgctta gcaaatgccc gaaaaataga aagccttagg ccttgaagta
      121 gacctttccc ctgttttact cgcgggtgaa gagaccatcc ctggtcactt tcgcttcgag
      181 tttgatggaa gattgaagga actagtcagt taatccaaat gcccgcacca ctgcgctgga
      241 aaggggattc tgtccggtta gctttcgctt gtattggctc aaatccaggg ccggacattg
      301 ttccaatcgc tcgaaacaga cggctccgcc ttcttccacc actgccttga aaagggcgta
      361 gtcgggtgaa gtcagcgctt gcaggctgtc tttcagcttt ccgagctgat tcatgggctc
      421 aatgaagccg tcaagctgct ccaggttatt cggtctcgtc tcggctgcga cagttctgta
      481 agagagaatc ttgttcagtg tgccgatttc gttccggaga gtttcctggg cccgcatcaa
      541 agatgccacg tagggtatgt ttacgacgcc gccttgcacc gggcagggta cgccgatgga
      601 acactgctgc gagctgaatt tgcttacgaa gagctccatc ttgtcggcat tgttgaggct
      661 tgccgtcatc cagcttatgg cttgcactgt caggctcaac cagaagatag tgcccatcag
      721 ggcttcatgg cgccaggcgt tcaaacgaag ttggaagtcc acaccggagc ggatggcaat
      781 cagcgggata agcgccagca caaccatgtg caaattggcg ctgatcagca tggtatccgt
      841 ggtaatggtt cccttgatgc cggtgcatac gagcaaagtt accagtggta ctaccaggcc
      901 gaggatgact gccagccagc ttgagacggc tttctcaaga ctgtttgctg ccagggttac
      961 tttagggatg tttgtttgca tgagaattct ccctgccagt tagagttaaa ccaacctacc
     1021 gtcggccatg atggagagca gatctttgtt gagagccgca ctacggtctg tgattgtctt
     1081 gagcgcatcc agattgaatt tcagccacgc ttcttgggca gacgagcttg ctctgctgtt
     1141 tgccacctgt tcgaaggcat caaccaattt gagtatgttg cgtgaattgt caaagctgag
     1201 tttgtaagct ccgctccagt gcaaggaagt cccaaatttc agcagttctg tcatttggtc
     1261 cacgaactga ctgtattgct cgaagtaggg ttcagccttg ttcagaccgg agtccgacat
     1321 catggctctg tcacccagta gattggcaat tcttgtcttg cagcgggcca gtatggcgga
     1381 ggacatgttt tcatgcactg gcgcaccgca cagttgccag agcaggtagg acagtgcctc
     1441 ttccagccat tccagagcgg gtcgaaaaag ggcagcgatg accgccaacc ccagcagcat
     1501 gatgagacca gccggtccgt acagaccaag aaattgcatt aggctttccg gttgggtgca
     1561 aattgcttca gtgttcataa atttctcccg atgaaggttg aaagctgcgc cagctattaa
     1621 tcgacaggat tgtcgccagg ctcagcttgt tttgaaaaat agattttgga aataccgccc
     1681 tttcttcttc ttttgaccat cacacaagct cagtccggta ggaaaggcaa ttacaggcga
     1741 tttgggccgg gcagatgtct gctcgggaga aaatcgctgt aaaggctgcc gtaaggcttt
     1801 ttgttattga gctgaggttc cggataaaag gtgaagatag ggtgctttca ccttacgctc
     1861 ttacatctcc agttccccaa gcgcttaaag ctgcatcaat ctggtgtttg ggcgctctat
     1921 ccagggcgga atggttgccc gctctgtatt ttgggggatt ctgggtgtga acggctatct
     1981 tcgttctttc tgccggcagc gtatattccg cagtagcagc caggctacgc agattacatg
     2041 ctactcaccg tctcttcgcg cggcatgttt ggcgtcctga tcataaacac agaatagaca
     2101 caacgcgact ttcccctgaa aacctgcatt tcccggccgg agcggaccaa tggtgccagc
     2161 ctcgcttggt aatcattagc gcagcttaat gaggtgcgcc agggggctgc ttcggtaggc
     2221 ggaaggaccg atcttaacta actggcgaag ctatgctggg taaggctttt ggggagctgg
     2281 ctatggtttt tcgggaacgc ttaagggttt gacggtggga tgcgctggcc gggccagggt
     2341 ctttacagga agttagaaat ttatgcctgg gaattctccc aatgagccta aacatttcct
     2401 ttatttttgg ttcttgaaaa cgttaagtat gggtatctct cgtcacattt atctccagga
     2461 catctcactc aactccaagc cttctcaggt ctagcaccta agcttcaaaa gcatccccct
     2521 cgggacgccc ggagttgaac tgaatgcttt ctgcacttgt tctggtggtc gatgtgtcac
     2581 cggtgcggct tttgcgagct gtgccagcgg cgggtcgaga gtgtatcgga gaggcctcgg
     2641 tggtctgtcc aactctttct ttatcaacct gaaaccaagg agtctgtcat gagcgctcaa
     2701 atcgctacgt cagtgtctgg tcgtcgtcgt cggttgaacc ccaaagttct gcccgacgcg
     2761 gtcatcggct tcaaggaaca ccttgacgcc aacctcttcg ggcagcagga aggcaaggat
     2821 cttctgacct tcgctttcac cgctgctcac aacccgctgc gcaaccgcaa caagcccatc
     2881 gcgttcatca tcaacgccgg caagtcgcgt accggtaaga cccactccgt ggaactgctc
     2941 gccgagcgca tccacgggaa ccccaaggct tacctgcgca tcgacatggc cgagtacatg
     3001 gacaagtact cgatctccaa cctcaagggc tccggccgcg gctacatcaa caaccagagt
     3061 gcccgcgacg aaggctacaa cgccgtggcc aaggacgtga agcacgacta cgccgagttc
     3121 accaaccaca acctggagtg gagcaagcgt ggttcgaccg tgccggtgtc catcgttctc
     3181 ctgg
//
LOCUS       DAOQNZ010000317         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15500_length_3171_cov_3.214698, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000317 DAOQNZ010000000
VERSION     DAOQNZ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15500_length_3171_cov_3.214698"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..904
                     /locus_tag="PKW73_06925"
     CDS             221..904
                     /locus_tag="PKW73_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73052.1"
                     /translation="MKFHSDLFGWEYVWRNFADEKGGAALTDSGEEEGKLLCLSVPVG
                     AAGARALFVPEAHGQKMTAGTVLLAYEPADDFAFSIFQEKMHHQLSKALGMQDLKVQD
                     ELFDSKFMIQGNDAVKVQEIFLDVQLRELVLLQPPATLHIDKKAGKTFPALALTDGQH
                     AVVAKYDGAMDKLHQLQTALDVVVGVLEQLTQIGSVAGVQAAKVAAEPEAAQSDTQSK
                     RLRSPLLDR"
     gene            1147..3009
                     /locus_tag="PKW73_06930"
     CDS             1147..3009
                     /locus_tag="PKW73_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNA73053.1"
                     /translation="MKNSAIIAGEKAAASCYGQNIEQAALIVLGDYGADGRVLVRKVL
                     KGDASLLGKPLTLPGPLTMGCRMQPVPPLKNVMLLLRSDHSLVEIYDLPEPIGYVSVL
                     VPIYGKTQERERLLSLLSLYKNHGKVGTTYNGEYKKEFLYAIRQMKEPANFEIVKNVY
                     LDEKVDAKDRLSLQEWLAETQDPRALPLLIGALDSHDKWLVNDAASKLICYFPGKETD
                     AAFAARLKTLPDDLRPMVVRTLLGRGVKLTGSVKLPETTLFQRAEALDKEGKKKEAGA
                     LYLQILESSESNGYILRIAALKCLDENSQAVRERIIKSRLDWLTEDAARGNYLEVTDT
                     ARILRKLKDERCLKGLMAILPRRDFIFARANLLASLAIAELGKDSAGQAASALLREMN
                     LSHKSELSTRSDEQVRLLLELGFLRQASAITEAQEFCLNPKNSNWASSYKSLEPVLKA
                     LQARDEGLSLTNLLKMQGSQKLPQQAVEYIIYRLGELKDARSTDVLLALFAARPAYSP
                     QTVNEALQSIGGPEIVSHMEATALDGKSPVQAEAIEVLSNMAPTRALPVLRKLVKNGA
                     IEVRVRALAGLSRLGNCDDYKMLAPLADYWKGDRPVHYWVLQARYNISERCRCH"
BASE COUNT          731 a          793 c          870 g          777 t
ORIGIN      
        1 ccaagcataa ccctcttaag ggcttgattt caggggctgg ccgtttctgc tttttgcttt
       61 taatgccggg gcgcttgtac ggaagccccc ggcgtctagc tttgcctgta aaggcttctg
      121 gcggtgaata tgaaacgaat aggcttgcaa agtgttgccg ggtctgtcta aacttcttcg
      181 gactaaagac ctgctcgtat cacgattttg gagaactgac atgaagtttc acagcgatct
      241 cttcggctgg gagtatgttt ggagaaattt cgccgatgaa aagggcggtg cggccctcac
      301 cgattctgga gaagaagagg gcaagcttct ctgtctttcg gtacccgtag gcgcagcagg
      361 cgccagggcc ctgtttgtgc cggaggcaca tggtcaaaag atgacggctg gaaccgtgct
      421 gcttgcttac gaaccggccg atgacttcgc attttccatc tttcaagaaa aaatgcacca
      481 tcaattgagc aaagcccttg gtatgcaaga cttgaaggtg caagatgagc tgttcgacag
      541 caagttcatg attcagggca acgatgccgt caaagtgcag gaaatttttc tagacgttca
      601 actgcgggaa ctggttttac tacagccgcc cgccacgctg cacatcgaca agaaagccgg
      661 caagactttc cccgctctgg cactgaccga cggacagcat gccgttgtcg ccaaatatga
      721 cggcgcgatg gacaaattgc accaattgca gacagctctg gatgtagtgg tgggagtgct
      781 ggagcaactg acacagattg gctcagtggc cggtgtgcaa gcggcgaagg tggcggctga
      841 gcctgaggcg gcccagagcg atactcagtc taaacgactg cgcagcccct tacttgatcg
      901 ctagtttggg cggaagataa tttactgggg tttttactgg tcgaccgacc tggtgcttgt
      961 gctaagctcg aaaaaatcag ccgtattaga gtttgccaga cgatgatatt cccagctagt
     1021 aaccggtccc agaaagcggc ttacatcttc gctcgaagct ttgctcttgg ccttaccctg
     1081 ccagttgtgc tgctttccat tgccctgccg tattctttgc ctgtaagttt tgccaaggcc
     1141 gctcccatga aaaattcagc catcattgcc ggagagaaag ccgccgcctc ctgttatggt
     1201 cagaacatcg agcaggctgc tttgatcgta ttgggcgact atggggccga tggccgggtg
     1261 ctggtgcgga aggtgctcaa gggcgacgct tcccttttgg gtaagccgct taccctgcct
     1321 ggaccgctca ccatgggctg tcgaatgcag ccggtaccgc ctctcaaaaa tgtaatgcta
     1381 ctcttgcgct ccgaccacag cctggtggaa atctatgact tgcccgagcc cataggctat
     1441 gtgagcgttc tggtgcccat ttacggcaag actcaggaga gggaaagact tctgtcgctg
     1501 ctttcgctct acaagaacca cggcaaggtc ggcaccacat ataatggcga atacaaaaag
     1561 gaatttcttt atgccatcag gcagatgaag gagccggcca attttgagat tgtcaaaaac
     1621 gtatatcttg acgagaaagt tgatgcaaag gacagattgt ctttacaaga atggctggct
     1681 gagactcagg acccgcgggc acttccgctt ctaatcgggg ctctggattc tcacgataaa
     1741 tggcttgtga atgacgctgc ctctaagctt atctgttatt tccccggtaa ggaaacggat
     1801 gctgcctttg cggcgcgtct taaaaccttg cccgatgact tgcgacccat ggtggttcga
     1861 actcttcttg gccggggagt gaagctcact ggcagtgtaa aactgccgga aaccaccctc
     1921 tttcagcggg ccgaggccct cgataaggaa ggtaagaaga aagaagccgg tgcgctctat
     1981 ttgcagattc tcgagtctag tgaaagcaat ggctatatat tgcgcattgc cgccctcaaa
     2041 tgtctcgatg aaaactcgca ggcagtcagg gagcgcatca tcaaaagtcg cctcgactgg
     2101 ctgacggaag atgccgctcg gggcaattat ctggaagtta ccgatacggc acgaatcttg
     2161 cgcaagctaa aggatgagcg ttgtttgaag gggctcatgg ccattttgcc ccgccgtgat
     2221 tttatcttcg ccagggctaa tctgcttgcc tctctggcta ttgccgaact cgggaaagat
     2281 tcagccgggc aagccgccag tgcgctcttg cgagaaatga acctttcaca caagagcgag
     2341 cttagcacta gaagcgatga gcaggtgcga ctgcttttgg aactgggctt tctcaggcaa
     2401 gcttcagcta taaccgaagc tcaggaattt tgtcttaacc ccaagaactc caactgggca
     2461 tcgtcttata agagcctgga accagtgctg aaagctttgc aggccaggga tgaaggactg
     2521 tcccttacaa atttgctgaa gatgcaaggc agccagaaac tgccccagca ggctgttgag
     2581 tacatcatat accgcctcgg ggaactgaaa gatgctcgca gcaccgacgt cttgcttgct
     2641 ctttttgcgg ccagacccgc atacagtcct caaacagtga atgaagcctt gcagtcaatc
     2701 ggcggcccgg aaattgtatc tcatatggaa gccaccgccc ttgatggcaa gtcgccggtg
     2761 caggctgagg ccatagaagt tctcagcaat atggcgccga ctagggcgct gcctgtttta
     2821 cgcaagcttg tgaaaaacgg tgccatcgaa gtgcgagtgc gggccttggc agggctctct
     2881 cgcctgggta attgcgacga ctacaaaatg ctggctcccc tggccgatta ctggaagggc
     2941 gatcgtccgg ttcattactg ggtgctgcag gctcgttaca acataagcga gcgctgccgc
     3001 tgtcactagg cggtttcttt gtgattgcta cttgtctccg tagggattgg tgaagtctcg
     3061 ggtgaaaacg gaattttatt agcagatgct cataatttcg cgtgttgttt tgcttatttg
     3121 tgattatact tgattgcaat tgattgtctt cttgcgtaac tgtctttgag c
//
LOCUS       DAOQNZ010000318         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15532_length_3167_cov_3.106362, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000318 DAOQNZ010000000
VERSION     DAOQNZ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15532_length_3167_cov_3.106362"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PKW73_06935"
     CDS             <1..617
                     /locus_tag="PKW73_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNA73054.1"
                     /translation="FAADGNLLTSDITLLKLEPKRSPDEIEVGLIKSGSAIAGDTDKL
                     ERLRQQVQSYLGSSVQVLNGPTFAEREKTYWAGSTGVGFIFGLGVLVGFIVGVVIVYQ
                     VLSSDVVDHLPEYATLKAMGYSDRYLFGIIMQEALILSVAGFLPGVLASAGIYEIAHA
                     ATLYPLSMTPERIALVFVMTFLMCAISGFIAARKLHSADPAELF"
     gene            639..1340
                     /locus_tag="PKW73_06940"
     CDS             639..1340
                     /locus_tag="PKW73_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA73055.1"
                     /translation="MQAEREEVIAVKDLNFSFRESGEKKSASLKQILFNIDLQIGAGE
                     IVIMRGPSGSGKTTLLTLMGALRTGATGRMCVLGENLIGASQETQMAVRRQIGFIFQA
                     HNLLSYLTVAQNVQMALQLARTFQAERQADREIGKVLKTVGLLDKLNTFPENLSGGQK
                     QRVAVARALVNKPRLVLADEPTAALDKSSGKEVVELMKALAKDRGTAVVLVTHDDRIL
                     DIADRIIYMEDGAIQ"
     gene            1414..2409
                     /locus_tag="PKW73_06945"
     CDS             1414..2409
                     /locus_tag="PKW73_06945"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA73056.1"
                     /translation="MPRTVLSTFLCLLLAFGLPFAAQSKELPLKPITIAQFGHVFLYM
                     PLYVAMRKGFFKEEGLDVRLVSTGGDEKTFTAVVTGNAQFGVSDPTFTAIARERGQGG
                     KVVAGIVRGVPFWVVAFDPSIKNFDNPEAFKGKRVATYTAPSTSYAVMKKVLQNNGHP
                     VEAKIVQGAFGTLLAMLKARHADMALDIEPIVSIATAEGARVVYSPAGKLGDFAFTGL
                     TVSDDYLKKNPETVQKAVNAITRAMAYIRKDYEGTVKIACADFPEVKEQVVREALKRL
                     LSQGTIPKDTLLPEAAWQNAVNLRREIGDLKSAGSFKENVDMSFARKALSVAGTK"
     gene            2406..2867
                     /locus_tag="PKW73_06950"
     CDS             2406..2867
                     /locus_tag="PKW73_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA73057.1"
                     /translation="MTALSIEALQFGFADADKVVNDFHLQAESGEIICLLGASGCGKT
                     TVLNLIAGLLKPSGGKIELSTRKSAAETGIDVGYIFQNDALFPWRTVEGNLLLASDLN
                     PILSKAQALDKMAQYLDVFHLSKSVLKKLFELYLMALSYSFSIFSLASFLS"
     gene            complement(2768..>3167)
                     /locus_tag="PKW73_06955"
     CDS             complement(2768..>3167)
                     /locus_tag="PKW73_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73058.1"
                     /translation="AKAMGYKTYYFDGQVSQLWNGKNSDISDYGEWITSKDWKSPNKF
                     DIDAEIAEKINEITTASTGNFIWINKVGVHKPYKNSYPNTETIWQPVSSDEDFTVIYD
                     KNEAKEKIENEYDNAIKYNSNSFFRTLFER"
BASE COUNT          795 a          830 c          800 g          742 t
ORIGIN      
        1 cctttgccgc cgacggcaat ctgcttacct ccgacataac ccttctgaaa ctggagccga
       61 agcgaagccc cgatgaaatt gaagtgggct tgatcaaatc cggctctgcc attgccggtg
      121 ataccgacaa attggaaaga ctgagacagc aagtacagtc gtatctgggc agttcggtgc
      181 aagtgctgaa cggaccgact ttcgccgagc gcgaaaagac atattgggcc ggcagcacag
      241 gcgtgggctt tatattcgga ctgggcgtat tggtaggttt catcgtcgga gtggtgatcg
      301 tctaccaggt gctttcttcg gatgtggtgg atcacctgcc ggaatacgcc acactgaaag
      361 ccatgggcta cagcgaccgg tatctcttcg gcatcatcat gcaagaagct ctgatcctgt
      421 ccgtagcggg cttccttcca ggcgttctcg cttccgccgg catttacgaa attgcccatg
      481 ccgccactct ataccctctc agcatgaccc cggagcgaat cgccctggtc ttcgttatga
      541 ctttcctgat gtgtgcgatt tccggattca tagcggcccg caagctacac tccgccgatc
      601 cggccgaact attctagaaa gctcaggagg caaaacttat gcaggcagag cgggaagaag
      661 tcatcgccgt caaggacctc aacttttcct tccgggaaag tggggaaaag aagagcgctt
      721 ccctcaagca aattctcttc aacatcgacc tccagatcgg cgccggcgag attgtaatca
      781 tgcgcggacc atcaggctcg ggcaagacca cgctgctcac cttgatggga gccctgcgta
      841 cgggagccac cggccggatg tgtgttctcg gtgaaaatct gatcggggca agccaggaaa
      901 cgcagatggc agtgcgcagg caaatcggct ttatctttca ggctcacaac ctgctttcct
      961 acctgaccgt ggcccagaat gtacagatgg cgctgcagtt agcgcggact ttccaagctg
     1021 agcggcaggc agatcgggaa atcgggaagg tcctcaaaac agtaggtctg cttgataaac
     1081 tcaacacctt cccggaaaat ctctccggtg ggcaaaaaca aagagtagcc gtggccagag
     1141 ccctggtgaa caaaccccgt ctggtgctgg ccgacgagcc taccgccgcc ctcgataaaa
     1201 gctccggcaa agaagttgtg gaactgatga aggccctggc taaagatcga ggcacggcag
     1261 tggtcctggt cactcatgac gaccgcatcc tggacatagc cgaccgcatc atctatatgg
     1321 aagacggagc gattcaatga ccggaccggg aagcttaggt ctctccataa tgtgcgaaaa
     1381 ttgcggctat tccgacaaca gtgaggtcca ggaatgccaa gaaccgttct gtccactttc
     1441 ttatgcctgc ttctcgcctt cggtctgcct tttgccgcgc aaagtaaaga gttgcccctt
     1501 aaaccgatta ccatcgccca gttcggtcat gtcttcctct atatgccact ctatgtggcc
     1561 atgagaaaag gtttcttcaa ggaggagggt ctggatgtac gcctggtcag caccggtggt
     1621 gacgaaaaga ctttcacggc agtggttacg ggcaatgctc aatttggtgt ttccgatccc
     1681 accttcacgg caatcgccag ggagcgggga cagggaggca aggtagtagc gggcatcgtg
     1741 cgcggggttc cattttgggt ggtggctttt gatccgtcca tcaaaaactt cgacaaccct
     1801 gaagcgttca aaggcaagcg agtagcaacc tacacagccc cttccaccag ctacgcagtg
     1861 atgaagaaag tgctgcagaa caatggtcac ccggtggaag ccaaaattgt gcagggcgct
     1921 ttcggcaccc ttctggccat gctgaaagcc aggcacgcgg atatggctct cgatattgaa
     1981 ccgattgtct ccattgccac cgccgaaggt gcgcgagtag tctatagccc ggccggcaaa
     2041 ttaggcgact ttgcttttac agggctgacg gtttccgatg actacctcaa gaaaaatccg
     2101 gaaactgtgc aaaaggcagt caatgccatt acccgagcca tggcttatat ccgcaaagac
     2161 tatgaaggca cggtgaaaat agcctgcgcc gactttcccg aagtcaagga acaggtggtg
     2221 agggaagcgc tcaaacgttt gctgagccag ggcactatcc cgaaagacac cctcttgcct
     2281 gaagctgcct ggcaaaatgc cgtcaatctg cgcagagaaa taggcgactt gaagagcgcc
     2341 ggcagtttca aagaaaacgt ggatatgagc ttcgcccgca aggcgctatc cgttgccggc
     2401 accaaatgac cgccctatcc atcgaagcac tgcaatttgg ttttgccgat gccgataaag
     2461 tggtgaacga ctttcacctg caagcagaaa gtggtgaaat catctgcctg ttgggtgcca
     2521 gcggttgcgg caagaccacg gtcttaaatc tcatcgccgg cttgctaaaa ccaagcggcg
     2581 gcaaaattga attatcgaca agaaaatccg ctgctgaaac aggcatcgat gtaggctaca
     2641 ttttccagaa cgatgccctc tttccctggc gcaccgtaga aggaaatctc cttctggcaa
     2701 gcgatttgaa ccctatcctg agcaaagcac aggcattgga caaaatggcc cagtatctgg
     2761 atgtctttca cctctcaaaa agcgttctga aaaaactgtt tgaattgtat ttaatggcat
     2821 tatcatattc attttcaatc ttttctttag cctcgttttt atcataaata acagtaaaat
     2881 cctcgtccga cgaaacaggc tgccaaatcg tttcagtatt tggatatgaa tttttatacg
     2941 gcttatgcac tccaacttta ttgatccaga taaaattccc ggttgaagca gtagtaattt
     3001 catttatctt ttcagcaatt tcggcatcaa tatcaaattt gtttgggctt ttccaatctt
     3061 ttgaggtgat ccattctcca taatctgaaa tatcagagtt tttcccattc caaagttgtg
     3121 atacttgtcc atcaaaataa taggtcttat atcccatagc tttagca
//
LOCUS       DAOQNZ010000319         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15603_length_3159_cov_3.560245, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000319 DAOQNZ010000000
VERSION     DAOQNZ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15603_length_3159_cov_3.560245"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1094
                     /locus_tag="PKW73_06960"
     CDS             123..1094
                     /locus_tag="PKW73_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA73059.1"
                     /translation="MTNKLNLPVVLSRSEILGPLAALLVVYLVFCWLSPSSFAGAANL
                     ELILRQSTIVGLVSLGMTIVIIAGGIDLSIGSVVALSTVVIASLLNAGFGPVLAATGG
                     IAVGALSGLANGFMVARFKVVPFIATLVSLLVLRGIAKGLAHEQKVDAPLTWLNELLA
                     RLDSTSAWQLVPGGVWLFLVMAVVVTVLLRYSRLGLHVFAVGSNVKTALLCGINVERV
                     ELWVYSLCGAFAGLAGLMQFSRLTVGDPTVASGLELDAIAAVVIGGASLKGGRGSVAG
                     TIIGALLMAVLRAGCTQIGLANWIQEIVTGLIILAAMGVDRLRYRQA"
     gene            1311..1865
                     /locus_tag="PKW73_06965"
     CDS             1311..1865
                     /locus_tag="PKW73_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73060.1"
                     /translation="MIDESVFSEQMQDQSRPIGYPLAVPAYAALEVFTLGIYTLYWSF
                     QHFSRAFHGTKAKVPRALICCFFLPLSLHSLMKYYDRCARSAGDRFRLPILPTALLFF
                     FNHLVSTFGSKYLPDYAPLINLVVAVTILGFIQNEVNRINREMRPDLLSSQVMTGRLW
                     IGLAVGFVVFFIIVGLFFVLALLR"
     gene            complement(1887..2849)
                     /locus_tag="PKW73_06970"
     CDS             complement(1887..2849)
                     /locus_tag="PKW73_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNA73061.1"
                     /translation="MSSYQNPAPDTVATKCRLVTRSDFDGLVCAVLLKHLNMIDNILF
                     VHPKDMQDGKVEVTDRDITTNLPYVEGVHLAFDHHLSETIRNVNKKNNHIIDPNAKSA
                     ARVVYDHFGGAKTFPARWNTMMEEVDKADAAQFSVEEILEPKGWVLLNYLMDPRTGLG
                     RFRNFRISNYNLMMDLIDYCKDHDIDDIMKMPDVVERVELYHDQTERFKEQVKRCSSV
                     HKNLIILNLKGEDVIYAGNRFMIYALFPQCNISMHVLWGLKQQNTVFAIGKSIIDRSS
                     KTKIGELCLKYGGGGHDNAGTCQIENERADAVMKELIEKINADG"
BASE COUNT          562 a          781 c          901 g          915 t
ORIGIN      
        1 ttccggccgg tgagcgaagt gagcgagttt tccgtgatgt ccgaagccac ggctcctgta
       61 cctggcttgg aaggtcagtc tgaatctatg acggagacaa aactatgcgc gatgtttcgc
      121 caatgacaaa caaacttaac ttacctgtgg ttcttagccg gagcgagata cttggacctc
      181 tggcggcgct cttagtcgtc taccttgtct tttgctggct cagtccgagc agttttgccg
      241 gagctgccaa tctagagctt atccttcgtc agagcaccat tgtcggactt gtttcactgg
      301 gcatgaccat agtcatcatt gccggcggta tcgacctttc tatcggttcg gtggtggcgc
      361 tttctacggt ggtcatagct tcgctcttga acgccggctt cggccctgta ctcgctgcta
      421 caggtggtat cgccgtggga gcgctctcgg ggcttgctaa cggcttcatg gtcgcccgct
      481 tcaaggtggt gccttttatt gccaccctgg tcagcttgct ggtcttgcgc ggcatcgcca
      541 aaggcctggc ccatgaacag aaagtggatg ctcctttgac ctggctcaat gagcttctgg
      601 cccgtcttga tagtacctcc gcctggcagc ttgtgcctgg cggggtctgg ctttttctcg
      661 tcatggctgt ggtggtgacg gtgcttttgc gctactccag attggggctg catgtctttg
      721 ccgtcggctc caatgtgaaa acggctttgc tctgcggtat caacgtagag cgggtggaac
      781 tctgggtcta tagcctatgt ggtgctttcg ccggtctggc cggattgatg caattttcgc
      841 ggctcaccgt cggcgatcct acagttgcct ccggtctgga actggatgcc attgccgctg
      901 tggtgattgg cggagccagt ctcaagggtg ggcgcggctc ggtggccggc actatcatcg
      961 gtgcactctt gatggccgtc ttacgggccg gttgtaccca gattggtctg gctaactgga
     1021 ttcaggagat agtgacgggg ctcataatat tagcggcaat gggtgtcgat cgtctgcgct
     1081 atcggcaagc atgagactga caagtgatta gctgaggacc aaggccgggt tggaaggaaa
     1141 gctctcaccc ggctttgcgt tttttttgtt ctttttgcta ctttttgacc tttttggcag
     1201 ccttttccct gccggtttgt tgatctccca tgcagtcctg ggatggccat cgggaattat
     1261 ggtagattct tggtaaaagt gacggcacca ggaagaaggg ctagaagatt ttgatcgacg
     1321 agagtgtttt cagcgagcag atgcaggatc aatcgaggcc tatcggttat cccctggcgg
     1381 ttccggccta tgcggccctc gaagtcttta cgctcgggat ctacaccctg tattggtcct
     1441 ttcaacattt cagtcgcgcc tttcatggta ctaaagcgaa ggtacccaga gctctgattt
     1501 gttgtttctt tttgccgctc tctctccata gcttgatgaa gtactacgat cgttgtgcca
     1561 ggagcgccgg cgatcgcttt agattaccta tactgcccac ggcacttttg tttttcttca
     1621 atcatttggt ttctaccttc ggcagcaagt acttgcctga ctatgccccg ctaatcaact
     1681 tagtcgtggc tgtgaccatt cttggtttta ttcagaacga ggtcaataga atcaatcgag
     1741 agatgcgccc agacttactt tccagtcagg tgatgacagg gaggctgtgg attggcctcg
     1801 ctgtcggctt tgtggttttt ttcatcattg tcggtctgtt ttttgtgctc gcattactgc
     1861 ggtagcagct tggcggctgt agcttcctaa ccgtcggcgt tgatcttctc gatcaactct
     1921 ttcatgacgg cgtcggcccg ctcgttttca atctggcagg tgccggcatt gtcgtggccg
     1981 ccgccgccgt acttgaggca gagttcgccg attttagttt tggaactgcg atcgatgatg
     2041 gatttgccga tggcgaagac cgtgttttgc tgcttcagac cccagagtac gtgcatggag
     2101 atattgcact gagggaagag ggcgtagatc atgaagcggt taccggcgta gattacgtct
     2161 tcgcctttga ggttgagaat gatcaggttt ttgtgcacgc tggaacagcg cttcacctgt
     2221 tctttaaagc gttcggtttg atcgtgatag agctctactc tttccaccac atcgggcatc
     2281 ttcatgatgt cgtcgatgtc gtggtctttg cagtagtcga taagatccat catcaagttg
     2341 tagttagaga tgcggaaatt gcggaagcgg ccaagaccgg tgcgggggtc cattaagtaa
     2401 ttgagcaaga cccagccttt cggttcgagt atctcttcta ccgagaactg ggcggcgtcg
     2461 gctttgtcta cttcttccat catggtgttc cagcgcgccg ggaaagtttt ggcgccgccg
     2521 aaatggtcgt aaaccactcg tgctgccgat ttggcgttgg ggtcgatgat gtggttgttt
     2581 ttcttgttga cgtttctgat ggtttcgctc aagtggtggt cgaaagccag gtgtacacct
     2641 tccacatagg gcaggttggt ggtgatgtcg cggtcggtta cttccacctt gccgtcttgc
     2701 atgtccttgg ggtgcacaaa gagaatgttg tcgatcatat tcaggtgctt gagcagcacg
     2761 gcacagacca gaccatcgaa gtcactgcgg gtaaccagtc tgcacttggt ggcgacggta
     2821 tccggggctg gattctgata gcttgacatg ggtttctccg cgagacatat gagacggttc
     2881 ttaattatac cttgccgata ttagcctgtg tatctccttg agatggcccg cctccgatgc
     2941 tgccggttat caggcgccgg gaaaaggctt agacaatggc tttccatgca attttgccgg
     3001 ggcttgctgc tttcttcaaa ttctcaactt ttgccattcc agccccttgc ctgccgcatg
     3061 gttgccaatt gttcaggggc gtgatctcaa gccccgacca gtgggacggt gattgagatc
     3121 acgtgtgtgg ttcgcgtctc gccgggtgtg catcttcag
//
LOCUS       DAOQNZ010000320         3151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15685_length_3151_cov_3.696059, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000320 DAOQNZ010000000
VERSION     DAOQNZ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15685_length_3151_cov_3.696059"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKW73_06975"
     CDS             <1..551
                     /locus_tag="PKW73_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73062.1"
                     /translation="ILFAFFGPYLNPRGFTFRIAILFMICGLSVTGTTEWMRELLRKP
                     YVVYNYMYSNGIRKTEVPDITSAGFLNKGKWASALANTPECKADPGEVIYRYQCMSCH
                     TVDGYRSMKRMIGDRDEESVVGFLNMLKEKDPEKNPYHGIMPPLAANDTELKSLAHYL
                     AGLNGKADGSAPKISHSAQSVH"
     gene            complement(570..1130)
                     /locus_tag="PKW73_06980"
     CDS             complement(570..1130)
                     /locus_tag="PKW73_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73063.1"
                     /translation="MKQPKQRTLMLCLLSGLISLTWTPCGKTPATAAGAEEKGRPAKA
                     AKPGASINFATGLLSSAQAQQILGGDVLPSPSNTYRDAKTGNATWVSNASFLLKENPA
                     VSLGLLIRHEESAASARARYEKALKDSKGSAVNGLGAPAYRTSKPPELNLLKGANWIV
                     ITAGTSEKANNGAEEKLAKAVLPKIK"
     gene            complement(1196..2635)
                     /locus_tag="PKW73_06985"
     CDS             complement(1196..2635)
                     /locus_tag="PKW73_06985"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA73064.1"
                     /translation="MAEFQIAIKYQPPALHRSFLAMCFLLPVWSILLPLGFGIATTWF
                     FLHLSEPVAPVAFVFSLIFLFLYIIGYLASAASEDKFIHVQKEGVAVPLFMLPYLSFK
                     RLQPWQELRAAHLVKTGDKQVLMLTFQDDKTIKLNTRHLDNSKLESFLLALELWGKSC
                     NRSPELLAFQSELQANASGTGELSYTHIWQDELSRRFASTSFVPLEPDQTLQQGKLKI
                     VRQLAFGGFSAVYLAQRNGHEQVVLKEAVVPENADPREKEVAENHLKRESLMLFAVKH
                     ESIARVEDYFVEDGRYYLVMEYVSGQDLRQLVRQHGPQSEEQVLNWAIQTASVLDYLH
                     KLDPPLIHRDLSPDNLVLSNEGAIKVIDFGASNEFVGTATGTLIGKQAYMAPEQIRGK
                     ASPASDVYALGATIFYLLTGKEPKPLTPARPRQFNDKVSEDLDNLVARCTAYEMEERI
                     ASASTLLDMLRALAGDTASTATNTVTNKV"
     gene            complement(2663..>3151)
                     /locus_tag="PKW73_06990"
     CDS             complement(2663..>3151)
                     /locus_tag="PKW73_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA73065.1"
                     /translation="LGKTFSIAEVKRLALEMCNILQYLHEQTPPVVHRDFTPDNLMLT
                     KAGALKLIDFNVANQQEETAAGTVVGKQCYMPLEQFQGNPSPQSDIYALGATLYYLLT
                     GLEPEPLTSSHPRESLVSSGLPEEAIDAIDALVAKCTAKNLSERFQNIEDLREALNRL
                     ND"
BASE COUNT          662 a          800 c          892 g          797 t
ORIGIN      
        1 ttattctctt tgccttcttt ggtccctatc tcaatccccg ggggttcacc ttccgcatcg
       61 ctattttgtt catgatatgc ggactctctg tgaccggaac cacggagtgg atgcgtgaac
      121 tattgcgtaa accttatgtg gtctacaact atatgtattc caacggtatt cgcaaaaccg
      181 aagtgccgga tattaccagt gccggtttct tgaataaggg caagtgggcc tctgctctgg
      241 ccaatactcc cgagtgcaag gccgaccccg gagaagtgat ttaccgctat caatgtatga
      301 gctgccacac cgtcgatggt tatcgcagca tgaagcgtat gatcggcgac cgggatgagg
      361 agagcgtagt gggcttcctc aacatgctca aagaaaaaga cccggaaaag aatccttatc
      421 acggcatcat gccgcctctg gctgctaatg atacggaact gaagagtctg gctcattatc
      481 tggccggttt gaacggcaaa gcggacggtt cggcgccgaa gatctcccat agcgctcaaa
      541 gcgtgcactg aatgttttaa gaaggtcagc tacttgattt tcggtagcac cgctttagcc
      601 aatttctctt ctgcgccgtt attggccttt tcactggtgc cggctgtgat gacaatccag
      661 ttggcacctt tcagcaaatt tagttccggc ggtttacttg ttcggtaagc cggtgctccc
      721 agtccgttca cggcgctgcc tttactatct ttgagggcct tctcatagcg agcccgggca
      781 ctggcggcag actcttcgtg cctgattaga aggccgagac tgacggcggg attttctttc
      841 aggaggaagc tggcattact aacccaggta gcattgccgg ttttggcgtc tcgataagta
      901 ttggacgggc tgggaagcac atcgccccct agaatttgct gagcctgggc cgagctaagc
      961 agtccggtgg caaaattgat gctggcacca ggcttggctg ctttcgccgg gcgtcctttc
     1021 tcttcggcgc cagcggcggt ggccggagtt ttgccgcagg gggtccatgt aaggctgatg
     1081 agacctgaaa gtaagcacaa catcagggtt ctttgctttg gttgcttcat tttgccggtt
     1141 tccataagtc tattttgtcg ggggctaatt tcagtttgcc tggataaggg caaacctaaa
     1201 ccttattagt cactgtattt gtcgcagtgg acgccgtgtc tcctgccagt gctctcaaca
     1261 tgtccaggag ggtggaagca gaggcaatgc gctcttccat ttcataggcc gtgcagcggg
     1321 ccaccaggtt gtccaggtct tcactgacct tgtcgttgaa ctggcgaggt cttgccggtg
     1381 taaggggttt cggttctttg cccgtcagta aataaaaaat ggtggcaccc agggcgtaga
     1441 cgtcgctggc cggactggcc ttgccgcgaa tctgttccgg tgccatataa gcctgcttac
     1501 ctattagcgt accggtggct gtgccgacga attcgttgga agcaccaaag tcgatgacct
     1561 tgatggctcc ttcattactg agcaccagat tgtccgggct caagtcccgg tgaatcaagg
     1621 gtgggtccag tttgtgtaga tagtccagca cactagccgt ctgaatggcc cagttgagca
     1681 cttgttcttc gctctggggt ccatgttggc gcaccagttg cctgaggtcc tgtccgctga
     1741 cgtattccat gaccaggtag tagcgtccgt cctccacaaa gtaatcttcc acccgggcaa
     1801 tactctcgtg tttgacggca aagagcatga gggattcccg cttgaggtgg ttctcggcca
     1861 cttctttctc tcgtggatca gcgttttccg gcaccacagc ctctttcaga acgacctgct
     1921 catgcccgtt cctctgggcc aggtaaacgg cggagaagcc gccaaaggca agttgcctga
     1981 ctattttcag tttgccctgc tggagcgtct ggtccggctc caggggcaca aaactggtgc
     2041 tggcaaagcg tcttgatagc tcatcctgcc agatatgggt ataggatagt tcgcccgtgc
     2101 cggaggcatt ggcttgcaat tccgactgaa aggccagtag ttccggcgat cggttgcaac
     2161 ttttgcccca cagttcaagg gccagaagga aactctccag cttggaatta tccagatgcc
     2221 tggtattgag tttgatggtc ttgtcgtctt gaaaggtaag catcagcact tgtttgtcgc
     2281 cggtcttgac caggtgagcg gcccgcaatt cctgccaggg ttgcaggcgt ttgaaagaga
     2341 gatagggcag cataaagagc ggtacggcca cgccctcctt ttgcacatgg ataaacttgt
     2401 cttcgctggc agccgaagcc aggtagccga taatgtagag gaagagaaat atcaaagaga
     2461 agacgaaggc caccggcgcc accggttctg atagatgcag aaagaaccag gtggtagcaa
     2521 tcccgaagcc cagtggcaat agaatgctcc agaccggcaa gagaaagcac atggccagaa
     2581 aggagcggtg cagtgccggg ggctggtact tgatggcaat ttgaaattcg gccatgcttg
     2641 atcctcgaat tggcgtttac ttttaatcgt tcaaccggtt cagggcctct ctcaggtcct
     2701 ctatgttctg gaagcgttcg ctcaggttct tggcggtgca ctttgccacc agagcatcaa
     2761 tggcatcaat ggcttcttcg ggaagaccgg aagagaccag gctctccctg ggatgggaag
     2821 aggtgagggg ctccggttcc agacctgtga gcaagtagta gagtgtagcg ccgagggcgt
     2881 agatatcgct ctgagggctg ggatttccct ggaactgctc caggggcatg tagcattgtt
     2941 tacccaccac cgtaccggcg gcagtttctt cctgctgatt ggccacgttg aagtcgatga
     3001 gcttgagggc accggctttt gtaagcatga gattgtccgg tgtaaaatcg cggtgcacta
     3061 ccggtggtgt ctgctcatgc aggtattgga gaatattaca catctccagc gccaatcgct
     3121 taacctctgc gatgctgaag gttttgccta g
//
LOCUS       DAOQNZ010000321         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15770_length_3144_cov_2.727744, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000321 DAOQNZ010000000
VERSION     DAOQNZ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15770_length_3144_cov_2.727744"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKW73_06995"
     CDS             <1..249
                     /locus_tag="PKW73_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73066.1"
                     /translation="GVTTSTPTAVSSRLIEMVSNTFSSCWVLVERMVKGQKGQTVHRV
                     WCCGTVVPPELGLKAGRVLAPQKAQKVKKVLKACGSWR"
     gene            complement(364..1026)
                     /locus_tag="PKW73_07000"
     CDS             complement(364..1026)
                     /locus_tag="PKW73_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNA73067.1"
                     /translation="MNTSKLSQSRLKNYIVKGALYLVPIALTLVILKVVLGLAYSIVG
                     PFLDAFSGLLLPKNADFMHGLFVGPIQSVLLLLAALTLLGIMASSNFGAKWLKSIDSL
                     FLRIPGVATLHSAGRRVSDLLMLQNNGAALRRVVTVQAVGNLRSIAFVTGETIDTNSG
                     KTFLHVILPFPPNPVMGLPGFIPAEEAIDCDMTVEQGLQYVISLGMAPVPNLKLSQAS
                     NQ"
     gene            complement(1131..2567)
                     /locus_tag="PKW73_07005"
     CDS             complement(1131..2567)
                     /locus_tag="PKW73_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73068.1"
                     /translation="MSFKTRASSIWSSVKRAWVNTFKFIGRGIKLSFSLTLVYVLVVP
                     VIQPALAMAWAHLPATIIAAAALVLVYLALRRKRYLLSALTLIAGLSTSAALVYLYHS
                     GWFIENEMASALNPRTLTELPDTINNRLLGRATALQYAGNANHDNRLRAGSPHLTASP
                     TGDKVSWQVPLHYTVWYGAALGSTGGLITIDAGQTSQKVDSIDHSFFPFGDESWLTEA
                     AFRLLHPLSRPGNVVHWRQDNGQWCILISYLSYKPTLGGGMIPYLAGVMQVNSSGTIA
                     HHSVDEAARLFPGVPLYPAPLARQYAEAYADYKLGVTNYLVTQKDLLRISEDPKLSDD
                     DTAFNRLPYYQVFQGLGLELVVPLEPNGKADNTALKEILFFDGSTGQAHSYQVSNNPP
                     VVGPKQALRNVVNADSQSDWGQFLPIESKLVVQGSKRYWLYTVVNRTTFAHVMLVLVD
                     GFTLEAHKFSSTDELHDFISRQGPNSNR"
     gene            complement(2810..>3144)
                     /locus_tag="PKW73_07010"
     CDS             complement(2810..>3144)
                     /locus_tag="PKW73_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73069.1"
                     /translation="LSQLFLYAFFVDWHGQWCWGTRYLTEVTPMVALMVLPVLSSALH
                     RWGFPIRFQGHGKRLQQKGSSTQSLTLPLWHSRSFNHENIAGLASNCKSQPIQTCRLF
                     AGNFAETG"
BASE COUNT          703 a          752 c          968 g          721 t
ORIGIN      
        1 ggtgtgacta ccagcacgcc cacagcagtg agcagcaggc taatagagat ggtttccaat
       61 acgttttcct cgtgttgggt tcttgtggag agaatggtta aaggtcaaaa aggtcagaca
      121 gtgcatagag tctggtgttg tggaacagtt gtacctcccg aattggggct taaagctgga
      181 agagttttgg cgccgcaaaa agcacaaaaa gtaaaaaaag tgctgaaagc ttgtggctct
      241 tggcgttaga gggatgagct cgttttgttt ggcagcacct ccctgcaagt gttaaggaag
      301 cacggaatta ctggatacgg aggtccgagt ccacgagact cagacctctg tatcctgcgt
      361 cggttattgg tttgaggctt gggagagctt caagttaggc actggtgcca ttccaaggga
      421 gatcacatac tgcagaccct gctctaccgt catgtcgcag tcaatcgcct cttctgccgg
      481 gatgaagcct ggcaggccca tgactggatt gggcggaaag ggcaggatta catgcaggaa
      541 ggtcttgccg gagtttgtgt ctatggtctc acctgtgacg aaggcaatgc ttctcaggtt
      601 gcccactgcc tgcaccgtaa cgactcgcct taatgcggcg ccgttgttct gcagcatcaa
      661 tagatcagaa acccttcgcc cggctgaatg cagggtggct actccgggga tgcgcaggaa
      721 aagtgagtcg atggacttca gccatttggc accgaagttg gagctggcca tgatgcccag
      781 caaggttagt gcggccagca gtaagagcac gctttgtatc ggccctacga aaagcccgtg
      841 catgaagtcg gcgttcttgg gcagaagcaa gcctgagaag gcatcgagaa aaggtcctac
      901 aatggaatag gccagcccca ggacgacctt aaggatcacc agagtgaggg cgattggtac
      961 cagatatagt gctcctttca ctatatagtt tttcaggcgc gattgagaca gtttggaagt
     1021 attcacagtc agaaaatctc tctggctttt gcagatgaat gatcgtcctt gatcgtgggg
     1081 cggtccttct tcagaccttt ttatgaaggg cgctccggcc tccggcttgc ttacctgtta
     1141 ctgtttggtc cttgccggga gataaagtca tgcagttcat cggtactgct gaacttatgg
     1201 gcttccaggg tgaaaccgtc taccagtacc agcatgacgt gggcaaaggt agtgcgattg
     1261 actacggtgt atagccagta gcgtttggag ccctgcacca cgagcttaga ctcgatgggt
     1321 aagaactgtc cccagtcgga ttgagaatcg gcgttgacaa cattgcgcag tgcctgcttg
     1381 ggccccacca caggtggatt gttggatacc tggtagctgt gagcctggcc ggtgctgccg
     1441 tcgaagaaga gaatctcttt cagggcggtg ttgtcggctt tgccgttagg ttccagcggc
     1501 accaccagct ccagaccgag accctggaaa acctggtagt agggcaggcg gttgaaggcc
     1561 gtgtcgtcgt cggagagctt gggatcctcc gatatgcgca agaggtcttt ctgggtgacc
     1621 aggtaattgg tcacgcccag cttgtagtcg gcgtaggctt cagcatattg tctggccagg
     1681 ggcgccggat agaggggaac gcccgggaag agcctggcgg cttcgtcgac ggaatggtgg
     1741 gcgatggtac ctgaggagtt tacctgcatg acaccggcca gatagggaat catacctccg
     1801 cccagggtgg gcttgtagct caggtaggag atgaggatgc accattgacc gttgtcttgc
     1861 cgccagtgga cgacattgcc tggtcttgag aggggatgga gcagacggaa ggccgcttct
     1921 gtcagccagc tttcatcacc gaaggggaag aagctgtgat cgatgctatc caccttctga
     1981 gatgtctgcc cggcatcgat tgtgataagt ccgccagtgg agccgagcgc ggcaccatac
     2041 cagacggtgt aatgcagagg cacctgccag gagactttgt cgccagtggg cgaggctgtc
     2101 aggtgggggc ttcccgctcg caggcgattg tcgtggttgg cgttgccggc atattgcaag
     2161 gctgtggccc gtccgagcag ccggttgttg atggtgtccg gaagttcagt cagggttcga
     2221 gggttgagag ccgatgccat ttcgttttcg atgaaccagc cgctgtggta gaggtagact
     2281 agagcggcgg aagtgctcag accggcgatc aaagtgagag ccgagagcag gtagcgcttg
     2341 cgcctcagag ccaggtacac gagtaccagg gctgctgcgg ctatgatcgt cgctggcagg
     2401 tgtgcccagg ccatggccag cgccggctga ataaccggca cgacgagcac gtatacgagg
     2461 gtcaggctga aggacagttt gattcctcgc ccgatgaatt tgaaggtgtt gacccaggct
     2521 cttttgactg agctccagat cgagcttgct cgggttttaa aactcacggt ttttgcctcg
     2581 tgggtttggt gactgggaaa actggcagat cactagcgag taggcctcac cgatgcagca
     2641 gatctgctct ggaagggttg ccgctgtggc ttgctgtttg aaaatctggt aagtggttgc
     2701 ctcgaactgg ttgggatgaa aattcatcta atgacccgta ttagcaagct gggctgggaa
     2761 aaaacaaatc aaaatttgtc tcgctttgcc ctttcgaatt ggtaaagaat caaccggttt
     2821 cggcaaagtt gcctgcaaag agccgacagg tttggattgg ctggctcttg caattgctcg
     2881 ctagccccgc tatgttttca tggttgaaac tgcgagaatg ccagagcggc agtgtcaggc
     2941 tctgcgtcga agaccctttt tgctggagtc gtttcccatg accctggaat cgaatcggaa
     3001 agccccaccg gtgaagcgca gacgacaaaa ccggcagcac catgagtgct accatcggag
     3061 tgacctcggt gaggtagcgt gtgccccaac accactgtcc atgccaatcg acaaaaaacg
     3121 cgtataaaaa aagctgcgaa agaa
//
LOCUS       DAOQNZ010000322         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15771_length_3144_cov_2.638718, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000322 DAOQNZ010000000
VERSION     DAOQNZ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15771_length_3144_cov_2.638718"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..630)
                     /locus_tag="PKW73_07015"
     CDS             complement(277..630)
                     /locus_tag="PKW73_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73070.1"
                     /translation="MKSTLLKGINSALAGFLISASLVVFPGVLFMRPSRQAGPAQMVL
                     ALNMTLEQQYWDKGLKTPKVLQDLMQQGFLLALARNEVDCLPFLFVGTCLGPLLMWIK
                     CQQPVKSEISDETNT"
     gene            806..1120
                     /locus_tag="PKW73_07020"
     CDS             806..1120
                     /locus_tag="PKW73_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73071.1"
                     /translation="MGSFFKKRRRVIIALAAVSLLCSFLTPYPMKVLRRVKRMVVSVV
                     ERKPVVEPPVIYTAEDQIATYGAAARARLQPKFQAKGVAYPPQRLTMVVIKSTKQLML
                     YA"
     gene            1178..1579
                     /locus_tag="PKW73_07025"
     CDS             1178..1579
                     /locus_tag="PKW73_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73072.1"
                     /translation="MLGPKLREGDCQVPEGLYNLTLEPNTPYHLALRLNYPNPTDWAH
                     AKEDGREQPGSDILVHGKECSIGCIAMGDPVSEDLFVAAYDTADKNLPLIIAPVDFRT
                     TAIPAASATDPKWLPELYGQIKAALAELPVN"
     gene            complement(2158..2715)
                     /locus_tag="PKW73_07030"
     CDS             complement(2158..2715)
                     /locus_tag="PKW73_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73073.1"
                     /translation="MYRVKTVSKNVYRSLHMGRAWLVCAGLLLTSPPAFAGGLTTAQL
                     NNVTLDAPGWEGDGVHHLKFTSGEYATESANGRILKTAVGDLDGDGQADGAVVYYENY
                     GGSGAFMRMAVFICKDGKPVQVGMRSLGDRSETKNLTIKNKALVLDIMTHRPNDSAPG
                     PTKHKVVNFKLKQGKLVGPEQVDWN"
     gene            complement(2748..>3144)
                     /locus_tag="PKW73_07035"
     CDS             complement(2748..>3144)
                     /locus_tag="PKW73_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA73074.1"
                     /translation="HSERVLVVLDTCHAGGASESKGLTRNANADALSFSQGTGQMVVC
                     SSDKNQVSWEGKNMQNSVFTRNLIDAFQEKGAQSPISEVFKTVKEKVEEQVVRERGVL
                     QTPVVEASKWSGNEIILGCRPLKPRPGKL"
BASE COUNT          731 a          796 c          769 g          848 t
ORIGIN      
        1 cgtaatgaga atcaagggca gaaccgcacc gaaagcagca gctccaacga tcgccctcag
       61 gcacctgccg agcaagccga ccatgaagag ggcgaaaata gataagcaat atcactgccg
      121 gttttcaaac ggggggaaga ggggccaaaa gcccctcttc gacttgcgtg cgagttgagg
      181 aggctacatc atggtaattt ggctaatgag aagcaggcgt gctaaaggtg gacttgccgc
      241 tgtggcgtaa gagtccgtcg gctttatttt agagtgttag gtgttggttt cgtcggatat
      301 ttctgatttc actggctgtt ggcactttat ccacattagc aaaggaccca ggcaggttcc
      361 gacgaagagg aatggtaaac aatcaacttc atttcttgca agtgccaaca gaaatccttg
      421 ctgcatcaaa tcttgtagaa cctttggtgt tttaagcccc ttgtcccaat attgctgctc
      481 taaagtcata ttcagggcaa gaaccatctg ggcaggaccc gcttgcctac tgggtctcat
      541 gaagagaact cccggaaaaa caaccagtga agcactgatc agaaaaccgg cgagcgctga
      601 attaatacct ttgagtagag tggatttcac gattctaaag caaccattac ccagtaaacg
      661 cgacctttgt gcgttctaca ccatcatagc aagttacgct acaagagcat tttggtgact
      721 gctctccttg ccagaccggc tggtgcaagc ttagaattca cttattgctg agttgtttgc
      781 tgagtttttt aaggattatt ggttgatggg aagttttttc aagaagcgac gccgagttat
      841 cattgctttg gcggctgtgt cgcttctttg ttccttcctg actccctatc ctatgaaggt
      901 gcttcgtcgc gtcaagcgga tggtagtgag tgtggtggag cgtaagccgg ttgttgaacc
      961 accagttatc tacaccgctg aagatcagat tgccacttat ggagccgcgg cccgagcaag
     1021 actgcaaccg aaatttcaag caaaaggggt agcctatccg cctcagcggc tgacaatggt
     1081 agtgattaag tccaccaagc agctcatgct ctatgcttga gccgctgatt cttcttacaa
     1141 atacatatgc tcttatcccg ttcttgcagc cagcggtgtg ctcggtccca aactcagaga
     1201 aggtgactgc caggtgccgg aggggttgta taaccttact cttgaaccca atacccctta
     1261 ccatttagcc cttcgcttga actacccaaa ccccaccgat tgggcccatg ccaaagaaga
     1321 cggtcgcgaa cagcctggta gcgacattct ggtgcacggc aaggaatgtt ctattggctg
     1381 catagccatg ggtgatcctg tcagtgagga tttgtttgtg gcggcttatg atactgccga
     1441 caagaatttg ccgctgatta tcgcgcctgt ggattttcgc accactgcca ttccggccgc
     1501 tagtgcaact gatccgaaat ggttaccgga actctatggt cagattaagg cggcattggc
     1561 ggagttgccg gtaaactagg gttttgtttt gtgcggaggg ctcctcaagc atcgccgtgc
     1621 tcaagtttgc gatcggtcta ccggtcctga tttgtccatc tccgaccata agacctggag
     1681 gtttatagtg agaggaattt tgatagttga tccagatgcc ttcgcagtgt tactttgcag
     1741 gattcgtcaa tgtatatgta caggtaattg tcgaatgcgc gtacgtctct gaattcctct
     1801 gtgtgaacgc cgccgtactg gtcataaaac gctatgtcaa ttggagtaaa ttctccggag
     1861 tgcgctagaa tcgtgatttt cttattcgcg ccgagttctc gaatagtagt tttacctaaa
     1921 ccataaccga gtgtgactct ctcaatagtt gatgggctgc agttctctat ttctaccagg
     1981 cagtctggag ggaaaagtat agtcccggta taactcattc caattagaag tgccgcaaat
     2041 gaacaaaaac cccaaatgaa gcccttctga aattgattcg ttgcaacgcc cattcctaac
     2101 tccttgagct ttatctaaat tacgtattga aataaaattc gccgctactc aagtacctca
     2161 gttccaatcg acttgctcgg gtcccaccag cttgccctgc ttgagtttga aatttactac
     2221 cttgtgcttg gtgggaccag gcgcactgtc atttggtcta tgcgtcatga tatcgagcac
     2281 cagggcttta tttttgatgg tcaggttttt cgtctcactg cggtctccga gagagcgcat
     2341 ccctacttgc accggcttgc catccttgca gataaacacc gccatgcgca taaaggcacc
     2401 agagccgccg tagttctcgt agtagaccac tgcgccgtca gcctgtccat caccatcaag
     2461 gtcacccact gccgtcttca gtattctgcc gttagcagac tcggttgcat actccccgct
     2521 agtaaatttc agatggtgca cgccgtcccc ttcccaaccc ggggcgtcta aagtgacatt
     2581 gttcagttgc gcggtagtca gcccgccagc aaatgcaggt ggtgaggtaa gcaatagacc
     2641 ggcgcagacg agccaggcac ggcccatgtg tagacttcgg tagacgtttt tagacactgt
     2701 ttttaccctg tacataagtc ctgccaatca tatcaattta gcactaccta aagcttgccc
     2761 ggtcttggct tgagcgggcg acagccgagg ataatctcat taccggacca cttgctggct
     2821 tccaccacag gtgtttgcag cacccctctt tccctcacca cctgttcttc cactttctct
     2881 tttactgttt tgaatacttc cgagattggg ctctgagcgc ccttttcctg gaaggcgtcg
     2941 atgaggtttc ttgtgaagac ggagttttgc atatttttcc cttcccagga aacttgattt
     3001 ttatcgctgg agcagaccac catttgaccg gtgccctgtg agaaactgag agcatcggcg
     3061 ttggcatttc tggttaagcc tttgctctca gatgcgccac cggcatggca ggtgtccagc
     3121 accaccagta ccctttcgga gtgt
//
LOCUS       DAOQNZ010000323         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15827_length_3139_cov_3.036316, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000323 DAOQNZ010000000
VERSION     DAOQNZ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15827_length_3139_cov_3.036316"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1072
                     /locus_tag="PKW73_07040"
     CDS             161..1072
                     /locus_tag="PKW73_07040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA73075.1"
                     /translation="MSAGEKAYQFNVSIVIVNWKTPLLLARCLDSLKEDPQYDTFELI
                     VVDNNSGDESVPMLKEKYPFVRLIENKDNAGFSRGCNQAIEIAGGRNVLLLNPDSVSV
                     KDAVSTMFKYLDDHPEVGAVGPRVLNPDGSLQLACRRAFPSVQASFFRLTYLSKLFPN
                     HPAVSRYNMTFADPDQFLDVDALSGSCMMLRRQVIDKIGLLDEDIFMFGEDIDWCWRV
                     KEAGYRVMYIPTASVFHIHGAASRKRPVGTTINLHKGMEVFYRKHLAKNYWAPFNFLV
                     YAAIWMRAAIFIVVNVLKGMLIKERSL"
     gene            1181..2194
                     /locus_tag="PKW73_07045"
     CDS             1181..2194
                     /locus_tag="PKW73_07045"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA73076.1"
                     /translation="MTSGGLKKILVTGGSGMIGSAVLSKLLERKGSLLKTQVRDRLAA
                     RAKLGKAMDITMVEFEQADFTRVGAQEMQALTKDCDTVIHCAGLVHNEGAAYQEYEVV
                     NVRATQLLAEACMATGVRTLIFLSTSAVYGKGPFKNIEESTPPQAKTPYAVSKVTSEN
                     YLQSLNGRIPRVVVLRPSLVFGEGDRGNMLSLIKQIQKEEYVHVSGGVTPKSLIYCQD
                     LADAILLAEAKAPAGFNIYNIANPSPVSTKELVDTIAEVLGVTKKFASVPEGLLKIGA
                     GIAKTFMPGKAPVTPEQIEKLATETTCSIDKFVQTTGFTPRTALKKGIANEVEWARAG
                     ELI"
     gene            2297..>3139
                     /locus_tag="PKW73_07050"
     CDS             2297..>3139
                     /locus_tag="PKW73_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNA73077.1"
                     /translation="MKTEILGGLTTFMTMAYIIVVNPAILAFAGIPVGPSTVATISTA
                     VFGCLLMGFFANRPLAVAPYMGENAFIAFGLAALGIGWQQRLGAVFISGLLFLAMALF
                     KIRPWLANSISLSMKHSFAAGIGMFLALIGLYETGIVESAVQGMPAQALLMPDGIKLG
                     NPPVPLKIGNLHDIKVLIAVFGTILTATLLCRKVRGAMLIGIAGSAILGYLLGVGEAP
                     KAIFSLPFTGDYSIAPIFMKMDVRDCLKLTFLPILLTLFLMSFLDTLSTIVGVGANCG
                     MLDKD"
BASE COUNT          751 a          927 c          775 g          686 t
ORIGIN      
        1 cccgcctctc taaagccgca caggaaagcg ataatctggt gcccatcatc tgcgaagccg
       61 tggaagccta tgcctcggtg ggtgaaatca ccggttctct cagacaggcc ttcggcaagt
      121 tccgccccat ggtcacaatc taaatagagg caagcctaaa gtgagtgcag gcgaaaaggc
      181 ctaccaattc aacgtcagca tagtcatcgt caactggaag acccccttgc ttctggcccg
      241 ttgtctggat tccctcaagg aagatcccca gtacgacact ttcgaactaa tagtggtgga
      301 caacaattcc ggcgacgaat cagtacccat gctgaaagag aagtatccct tcgtgcgcct
      361 gatcgaaaac aaggacaacg ccggtttttc ccggggctgc aaccaggcca ttgaaatagc
      421 cgggggacgc aacgttttgc tcctcaaccc tgactccgtc tcggtcaagg acgccgtttc
      481 caccatgttc aaataccttg acgaccatcc ggaagtaggc gccgtcggac cacgggtact
      541 caacccggac gggagcctgc aactggcctg tcgccgggcc tttccctcgg tgcaggcctc
      601 cttcttccgc ctcacctacc tatccaagct cttccccaac cacccggccg tgtcccgtta
      661 caacatgacc tttgccgacc cggaccagtt tcttgatgtg gacgcccttt ccggctcctg
      721 tatgatgctg cgtcgccaag tgatcgacaa aattgggctg ctcgatgaag acatctttat
      781 gtttggcgaa gacatagact ggtgctggcg tgtgaaagaa gccggctacc gggtcatgta
      841 tattccgacc gcctcggttt tccacattca cggagcggcc agccgcaaac ggccggtggg
      901 gaccaccata aaccttcaca aaggcatgga agttttctac aggaaacact tggcaaaaaa
      961 ctactgggcc ccattcaatt tcctggtgta tgctgccatc tggatgaggg cggcaatttt
     1021 tattgttgtc aacgtgctga aaggaatgct cattaaagag cgttccctat aggctgatat
     1081 ctgcatagcc gggcataatt caaaatagac agagaaaata gactatccaa actaattcaa
     1141 aattcggttg gcaatcaaaa aaggtaactg gcagcaaaat atgacttcgg gcggcttaaa
     1201 aaaaatcctg gtaaccggcg gcagcggcat gattggctca gcagtattga gcaagctctt
     1261 ggagcgaaag ggctccctcc tcaagaccca ggtaagagac agactggcag cccgcgccaa
     1321 gcttggcaaa gctatggaca ttaccatggt ggaattcgag caagccgact tcacccgggt
     1381 gggtgcccag gaaatgcaag ccctcaccaa agactgcgac accgtcatac attgtgccgg
     1441 tctggttcat aacgaagggg ccgcttatca ggaatacgaa gtcgtgaatg tgcgcgccac
     1501 ccagcttctg gccgaagcat gtatggcaac cggcgtcaga acattgatct tcctttccac
     1561 ctcagcggtt tacggcaaag gaccattcaa gaatatcgaa gagtcgactc cgccccaggc
     1621 caagacacca tatgcagtat caaaggtaac aagcgaaaac tacttgcagt ctctgaacgg
     1681 acgcataccg agagtggttg tcctgcgccc ctctcttgta ttcggggaag gagaccgtgg
     1741 caatatgctt tccctgatca aacaaattca gaaagaagaa tatgtacacg tttcgggtgg
     1801 tgtcacgccc aagagcctga tctactgcca ggatctcgcc gacgccatct tgctggccga
     1861 agccaaggcc ccggctggtt tcaacatata caacatagcc aatcccagtc ctgtatccac
     1921 aaaagaactg gtagatacca tcgccgaagt gcttggtgtc accaagaaat ttgcctcggt
     1981 accggaaggt cttcttaaaa tcggcgccgg tatagccaag accttcatgc ccggcaaagc
     2041 cccggtcacc cccgagcaaa tcgaaaagct ggccaccgag accacctgtt ccatcgataa
     2101 atttgtgcag accacaggct tcacgccccg caccgccctc aagaagggca ttgccaacga
     2161 agtagaatgg gcccgggccg gcgagctgat ctaattgaac gaacggctat aaagatcggc
     2221 ggcatccatt agcaccggca attgaaaggc tgaggtcatg gaaaaattct tccagttaag
     2281 cgcccggagc acttctgtga aaacagagat cctgggcggt ctgaccacct ttatgaccat
     2341 ggcttatatc atcgtcgtca atccggctat cctcgctttt gccggtattc cggttgggcc
     2401 cagtacggtg gccaccatca gcaccgctgt tttcggctgc ctgctcatgg gcttcttcgc
     2461 caatcgcccc ctggcggtag caccttacat gggcgaaaac gcctttatcg ccttcggtct
     2521 tgccgccctg ggcatcggct ggcaacagcg tttgggagcc gtcttcattt ccggtctcct
     2581 ctttctagcc atggccctct ttaaaatccg cccctggctg gccaattcca tttccctttc
     2641 catgaagcac agcttcgccg ccggcatcgg catgttcctg gccctgattg ggctttacga
     2701 aaccggcatc gtggaaagcg ctgtacaggg catgcccgcc caggccctgc tcatgccgga
     2761 cggcatcaaa ctgggtaatc cgcccgtgcc ccttaaaatc ggcaacttgc acgatatcaa
     2821 agttttgatt gccgtcttcg gcactattct cacggccacc ctgctctgcc gcaaggtcag
     2881 gggagccatg ctcatcggta tcgccggcag tgccatactc ggctacctgc taggcgtggg
     2941 cgaggcaccc aaagccatat tctccctgcc cttcacaggc gactacagca tcgcccccat
     3001 cttcatgaaa atggatgtgc gcgactgcct gaagctaacc ttcctgccca ttttgctaac
     3061 tcttttcctc atgagtttcc tcgacaccct ttcgaccata gtaggggtag gtgccaattg
     3121 cggcatgctc gacaaagac
//
LOCUS       DAOQNZ010000324         3126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15944_length_3126_cov_3.052426, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000324 DAOQNZ010000000
VERSION     DAOQNZ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15944_length_3126_cov_3.052426"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PKW73_07055"
     CDS             <1..646
                     /locus_tag="PKW73_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73078.1"
                     /translation="QLHEIEDCIPIYRQVLALQPQEPAASYNLGMWLLEKKDEEGIEH
                     IRAAIRARYALLPDAYPLWHSYLTEQGRVAEAEKLKQELDIYYKCADLARKERNNLNN
                     DSPIQAYELEQSHLEYLQEVFRALPAVHEVYLVQRILVHMPEYPYLVLGIEMPSGAFS
                     GTDDSDKVEMARALLDNLQMPAEFCVTVFDLGTIKLKNRMKAIEGSLIYRKAAK"
     gene            complement(621..1541)
                     /locus_tag="PKW73_07060"
     CDS             complement(621..1541)
                     /locus_tag="PKW73_07060"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA73079.1"
                     /translation="MEKPSLPHWYMRAVRFSAFALVCLYVGLSPYVLFQRFEFFPVKV
                     TAYKVPVAICDLTREEVSFEAGNGCKLRGWYLPRAKAKYTVILHHGQGANIAFDGYAE
                     TAAVLVKAGASVLLYDFEGFGRSDGSPSNQALSRDALAAYKFLCSRGVKGESIVHFGL
                     SLGTGAASQVASSKPCAGLILVSPYMSLSRVAAHHLPYLKFYPDWLYPQPNLGGQCLN
                     SFKQPVMIFHAAGDRLLPVGQADELAQKLLKQGCRVEYFRIEQDGHLGALSGAHSDIA
                     GGVVDICRRFLKHLENSTSEPSLTSLPCGK"
     gene            complement(1638..>3126)
                     /locus_tag="PKW73_07065"
     CDS             complement(1638..>3126)
                     /locus_tag="PKW73_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73080.1"
                     /translation="KQPNERYQDMNQLKHDFERILAGKEIFAPQTGSRVTTELKKPSG
                     SFTFGLPTMEFPVNAAGFAAPTSDLPARQQIAEGGTARILDRKLTTSEASVEELPART
                     NAGLLGVGLKVAVLMALVLGAAAALTYAYLRKPTEPERILAGQIKRDLVEINTPIGVG
                     VKPYAMNSIFKSPRQVAGGGLDLAGATEAEFDKVDTYYFIRYKPAEQAFRFALQKFLA
                     QKTQRYYLKSRGVFKFPDQFILGEISVAGRKPVHALGTVSAPPGSDVVFYLNAGTREY
                     PQVIYGFGPDDLTGLEMQTEEPRRILTEVKSWRRLKHLSFFNSLTKVWPGFERYDESP
                     ISDRFLGEINSLSSLTSLGLCGPKVTGKAILQMSLLKQLRCLQLKRIADAEPLLASLE
                     KYPNLKEIWLVAMDTTDDDLEYLVKMKNLETLRIRRGHITPSSLGYFKRMSKLKRLYI
                     DKHWTESDLSAFKKALPFCQFESIYDVSYMELLPKQETSRIPEDY"
BASE COUNT          827 a          820 c          769 g          710 t
ORIGIN      
        1 ccagttgcat gaaatcgaag actgcattcc aatttacaga caggtgctgg ctttgcaacc
       61 acaggagccg gccgccagct ataacctggg tatgtggctg ctggagaaaa aagatgagga
      121 aggcattgag catatccgcg ccgccattag ggcccgctat gcgcttctgc ccgacgccta
      181 tccgctctgg cacagctatc tgaccgaaca gggaagagta gccgaagccg agaaacttaa
      241 gcaagaactg gacatttatt acaaatgcgc cgacctggcc agaaaggagc gcaacaacct
      301 gaacaacgac tctccaattc aggcttacga actggagcaa tcccatttgg aatatctgca
      361 agaggtattc cgcgcattac cggcggtgca tgaggtctac ctggtgcaac gcattctcgt
      421 acacatgccc gaatatccat acctggttct gggtattgag atgccttccg gtgccttctc
      481 cggcacagac gacagcgaca aggttgaaat ggcacgggcc cttctggata atttacagat
      541 gcctgcggag ttctgtgtaa cagtctttga tcttggcacc atcaaactca aaaatcgaat
      601 gaaggccatc gaaggttccc tcatttaccg caaggcagcg aagtaagaga aggttcactg
      661 gtggaatttt ctagatgttt caagaatctc cggcaaatat caacaacacc gccggcgata
      721 tccgaatgcg caccggagag agcgcctaga tgcccatctt gttctatacg aaaatattcg
      781 acacggcagc cctgcttcag taacttttga gcaagctcat cggcttgccc gacaggcaat
      841 aagcgatctc cggcggcatg aaagatcatt accggttgct tgaaggaatt gagacactgc
      901 ccacctaaat tgggctgagg atacagccag tccggataga acttcagata cggcaagtga
      961 tgggcggcca ctctgcttaa ggacatatag ggactgacca ggattaaacc ggcacagggc
     1021 ttggaagaag caacttgact ggcagcgccc gttccaagcg ataatccaaa atgaacaata
     1081 gattcgccct tgacgccacg actacaaagg aatttgtagg cggctagagc atcacgactg
     1141 agagcctgat tggatggact accgtcactt cgtccaaagc cttcaaaatc gtagagaagc
     1201 acggaggctc cggccttcac caggacggcc gccgtctcgg cataaccgtc aaaggcaata
     1261 ttggcgccct gcccatgatg caatataacg gtatattttg cttttgctcg gggtaaatac
     1321 cagcccctca gcttgcaccc gttaccggct tcaaaactga cttcctcgcg ggtcagatcg
     1381 cagatggcaa caggcacttt gtaggcagtt accttaacgg gaaaaaactc aaaccgttga
     1441 aagagaacat aagggctcaa acccacgtaa aggcaaacca gggcaaaagc tgaaaaccga
     1501 accgctctca tgtaccaatg cggtagcgat ggtttttcca tgctgttttc cccgaccaag
     1561 attcgtcttt ataaaggcat tgtccggggg ctggctgtta cctgagagca atgaccaaca
     1621 ctctattaag tgttgcctca atagtcttct ggaatccggc tggtctcctg cttcggcaga
     1681 agctccatat aggaaacatc atagattgac tcgaactggc aaaacggcag agccttctta
     1741 aaggcagaca gatcagattc agtccagtgt ttatcaatat aaagccgctt tagcttgctc
     1801 atgcgcttaa aatagccaag ggaggaggga gttatatgcc ctctgcgaat acgcagggtc
     1861 tccagattct tcattttgac cagatattcc aggtcatcat cagtggtgtc catggctaca
     1921 agccagattt ctttcagatt gggatacttc tctaaagagg cgagaagcgg ctcggcatcg
     1981 gcaatacgtt tcaactgcag gcatcgcaat tgcttcaata aagacatttg caaaatggct
     2041 ttcccggtca ccttcgggcc gcagagtccc aacgaagtca ggctggatag actatttatt
     2101 tcccccaaga accgatctga gatggggctt tcatcatagc gctcaaagcc aggccagacc
     2161 ttggtcaagg aattaaagaa ggagagatgc ttgagccgtc tccagctttt cacctcggtc
     2221 agaatgcgcc tcggttcttc cgtttgcatt tccagaccag tcaggtcgtc tggtccgaaa
     2281 ccgtatatta cctggggata ctccctggtg cccgcattta gatagaaaac cacgtcgctg
     2341 ccgggaggcg ccgaaactgt tcccagagca tgaaccggtt tgcgcccagc cactgaaatc
     2401 tcacccagaa taaactgatc cggaaatttg aaaacccctc tggatttcaa ataatagcgc
     2461 tgggtcttct gagccagaaa cttttgcagc gcaaaccgaa aggcctgctc ggccggtttg
     2521 taacgaataa aatagtaggt atcgacctta tcgaactctg cttcagtggc accggcaagg
     2581 tcaagtccgc ctccggcaac ttgacgcggt gacttaaata tagagttcat ggcatagggc
     2641 tttactccaa cgccaattgg agtattaatc tccactagat cgcgcttgat ctgtcctgcc
     2701 aatatccgct ccggctctgt tggctttctc aaatacgcat aagtaagggc agcagcggca
     2761 ccaagcacta aagccataag caccgccacc ttgagtccaa caccaagcag cccggcattg
     2821 gtccgggcag gcaactcctc aactgaagcc tcggaggtag tgagctttct gtccaaaatc
     2881 ctggcggtgc cgccctcggc gatttgctgc ctggcaggca aatcgcttgt gggagcggca
     2941 aaacctgccg cattgactgg aaattccata gtgggcagtc cgaaggtaaa gctgccgctc
     3001 ggctttttca gctcagtggt aactctagag ccggtttgag gggcaaaaat ctccttgcct
     3061 gcaagaattc gctcaaagtc gtgcttcaac tggttcatgt cctgataacg ctcattaggc
     3121 tgttta
//
LOCUS       DAOQNZ010000325         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_15958_length_3124_cov_4.195829, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000325 DAOQNZ010000000
VERSION     DAOQNZ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15958_length_3124_cov_4.195829"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKW73_07070"
     CDS             <1..509
                     /locus_tag="PKW73_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73081.1"
                     /translation="PLSDKKGVQDYFRNCARRSGGGLVSVDMVKVKEVPCIMTIVKVP
                     QKPSGMTYIGSITIPLAEGSYVIRVQCIETGITGSRESAVLQNLTTSGKVGIDGHGNL
                     TNWQQDPYDRSLTAPDLILRNQSDDEKYDKVFPTHPLTKVRFGLRTILRTLELDKSVL
                     LAPPFLGP"
     gene            complement(513..1367)
                     /locus_tag="PKW73_07075"
     CDS             complement(513..1367)
                     /locus_tag="PKW73_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA73082.1"
                     /translation="MRAKIRDTQIYFDVEGAGLVPDGPRMNEKPVLFAVHGGPGVDHT
                     GYKPSLSPLSRHVQIVYFDHRGQGRSARGDIASYTLENNVADMDALRDYLGLEKIAVL
                     GGSYGGIVAQAYAAQYPDRVSHLILSVTASEGLCLTRAREILNERGTPEQIQWGERLF
                     QGAFESDEQVKEFFQVMGTMYSVKFDAEQAKLKRERSIVSKDAINFAFGGFLRQLSLT
                     ASLPRIKARTLVIGARHDWICAPEFSELVASKIDGAMLHIFEESGHWVAADQQQAYLE
                     LITNFLTS"
     gene            complement(1432..2070)
                     /locus_tag="PKW73_07080"
     CDS             complement(1432..2070)
                     /locus_tag="PKW73_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73083.1"
                     /translation="MGTAGAKEIILPLSGPYKTIDTKLATATIKLLQSKSKASQDKAI
                     KAIEAAPDRYAPPVLYQLSATLFQRGDKDKAAFWFYAGQLRGRYDANRCGDLSARSAI
                     AVMNDQFGQPINQYMFSNLSKLEKVVPEVLAWDKKTAHNYDQRWINLHGMGSMLTSLG
                     DKDQSKKDPGEVTMSLPQTEWPQIEEETRKNYLKGFQETLSEAKLLAKNKKQ"
     gene            2397..>3124
                     /locus_tag="PKW73_07085"
     CDS             2397..>3124
                     /locus_tag="PKW73_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA73084.1"
                     /translation="MGSRVLVPVDNQECSEAALLSVMSRVWEPDTSFLLCKVVEDFRA
                     ILGEGQVEHESALNAEQEEYRYNAGLWLKQATRTFAEVLPRVEACLEFGQVVEKLSDV
                     AYSWGADYIVLGSQDLGLSTRCALGSVASSLLRQAPCTVEAVRSSRLKHLLHAQGSVS
                     THEISQIITAPPRKIIIATDLSSGADAAAEWVAEMNWSPDAEFLVATVNTPSHKDSHS
                     HLLSMGAAYTREKYHLLQVESLLK"
BASE COUNT          664 a          806 c          861 g          793 t
ORIGIN      
        1 gccccttaag cgacaagaaa ggcgtgcaag actattttcg caattgtgcc agacggagcg
       61 ggggcggtct ggtgtcggtg gatatggtca aggtcaagga agtgccctgt atcatgacca
      121 tagtcaaggt tccccagaag cccagcggta tgacttacat aggctccatc accattccat
      181 tggccgaggg cagttatgtt atccgggtgc agtgtattga aaccggtata accggcagcc
      241 gtgaatcagc cgtgctgcag aatctcacca ccagcggtaa ggtcggcatc gatggtcacg
      301 gtaacttgac gaattggcag caagatccct atgaccgtag tttaaccgct cctgatctga
      361 ttttgcgcaa tcagtccgac gatgagaagt atgacaaagt tttcccgact catcccctca
      421 ccaaggtgcg cttcggtttg cgtaccatat tgcgcactct ggaactcgac aagtccgtgc
      481 ttctggcgcc gcctttcctt ggtccctgag cgctagctgg tcagaaagtt tgttataagt
      541 tccaagtagg cctgctgctg atccgcggcc acccagtggc cgctttcctc gaagatgtgc
      601 agcatggcac cgtcaatctt agaggccacc agctccgaaa actccggggc gcagatccag
      661 tcatggcgag ctccgatcac cagagtgcgg gctttgatgc ggggcaacga agcagtcagg
      721 gaaagctgtc tcaaaaaacc accaaaggca aagttgatgg catcctttga aacgatgctg
      781 cgctctcgtt ttagctttgc ctgctcggca tcaaacttga ccgagtacat ggtacccatc
      841 acctggaaga actcttttac ctgttcgtca ctttcaaacg ccccttgaaa gaggcgctcg
      901 ccccactgga tttgctcagg tgtgccgcgt tcattgagaa tttcccgtgc tcttgtgagg
      961 caaagaccct cggaggcggt caccgataaa atcaggtgcg aaactctgtc gggatattga
     1021 gcggcatagg cctgggccac gataccgccg taggaaccgc ccaggacggc gattttctcc
     1081 agtcccagat agtccctgag ggcatccatg tcggccacgt tgttttcaag cgtataagag
     1141 gcgatgtcgc ctcgggcaga gcgaccctgt ccgcgatggt cgaagtagac aatttgcaca
     1201 tgtcggctga gggggctcaa gcttggcttg tagccggtat gatcgacgcc gggaccgcca
     1261 tgaacggcaa aaagcacggg tttttcattc atacgaggtc cgtccggtac cagcccggct
     1321 ccctctacgt cgaagtaaat ctgggtgtca cgtattttgg cgcgcacggc tgcctgtcct
     1381 ctaatgttgt atttacaaag cctgcctgtc tcggtttctg cccgggcagg cttactgttt
     1441 cttgtttttg gcgagcaact tggcttcact gagggtctcc tggaaaccct tcagatagtt
     1501 ttttctggtt tcttcttcta tttgcggcca ctccgtctgg ggcaggctca tagttacttc
     1561 cccgggatcc tttttgctct ggtccttgtc gcccaggctg gtcagcatcg atcccatccc
     1621 gtgtaagtta atccagcgtt gatcatagtt atgggcggtt tttttatccc aggccagtac
     1681 ttccggcaca accttctcca acttgcttag attcgagaac atgtactggt taataggctg
     1741 gccgaactga tcgttcatga cggcgatggc tgacctggcc gagaggtcgc cgcagcgatt
     1801 ggcatcgtat ctacctctca actggccggc gtagaaccag aaggcggctt tgtctttatc
     1861 gccgcgctgg aaaagggtgg ccgacaattg ataaaggacc ggcggtgcgt agcgatcagg
     1921 ggcggcttct atggctttta tggccttatc ctggctggct ttagacttgg attgcaagag
     1981 cttgattgta gctgtggcca gttttgtgtc gatggtcttg tatggtccgg aaaggggcaa
     2041 aattatctct tttgctcccg ctgtgcccat cgctgaaaaa atgctggtcg atatggcaca
     2101 cgcaagcaag attgacttga tttctgatct cattctattc aaccgattgg cagcttaaca
     2161 gccgtgatta tacaagagcg tgagcggctg cgtttggctc ttgctgtctc taatttgacg
     2221 ggaggcattc tgattgtttg ctccgactgt gagcgaaacc ggtttccttc ggggaacctg
     2281 agcttcccgg cagagagcaa cttggggaca aatcagcctc ttgatcagtc tccggtttga
     2341 tcaattgtgg agtggttcgg gctatcattg agacgatggt ggtgaaggct gttgaaatgg
     2401 gatcccgagt tttagttcca gttgataatc aggagtgctc cgaagcagca ctgctctcgg
     2461 tgatgtctag agtctgggag ccggatacaa gttttcttct ctgtaaggtg gtggaagatt
     2521 ttcgggccat actgggagaa ggacaggtag agcacgaaag cgccctgaat gccgagcagg
     2581 aagagtatcg ctataacgcc ggactctggc tcaagcaggc cacaagaacc tttgccgagg
     2641 tgttgccgcg ggtggaagct tgcctggaat tcggtcaggt agtggaaaag ctctctgatg
     2701 tagcttacag ctggggtgcc gattacatcg tcctcggttc gcaagacctg ggactttcca
     2761 ctcgctgtgc tcttggtagt gtcgcctcat ccttactgcg gcaggctcct tgcacagtgg
     2821 aggctgtgcg tagcagtcgt ctcaaacatc tgctgcacgc tcagggaagt gtcagcaccc
     2881 atgagatcag tcagatcata actgctccac cccgcaaaat tatcattgcc actgatcttt
     2941 ccagtggtgc cgatgccgca gcggaatggg tggctgaaat gaattggagt cctgatgccg
     3001 aatttcttgt ggctaccgtc aataccccat cgcacaaaga ttcccattcc catctgctca
     3061 gtatgggagc tgcctacacc cgcgaaaagt atcacctgtt gcaggtggaa agcctgctta
     3121 agaa
//
LOCUS       DAOQNZ010000326         3117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16028_length_3117_cov_3.566623, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000326 DAOQNZ010000000
VERSION     DAOQNZ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16028_length_3117_cov_3.566623"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="PKW73_07090"
     CDS             <1..1853
                     /locus_tag="PKW73_07090"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNA73085.1"
                     /translation="MLRIRGGGGSYDRRWHRPLSVGFMPPMGAGILSVALSLSLGFVG
                     AIWVAPTACAEDGAASDSATAEPENAESKSIVLTAMEAELERSMRKLKNAAAAPLYYL
                     SYSVYDVQTLDLGASYGALGYDDDSRSRNLYVELRVGDKTLDNTHQFRSQAPAGSPLA
                     FGRGYSGGGSFPLEDDRDAIRTRLWDATDGAFKQAQIDYMRVKTNRDVRVAEEDASSD
                     FSAATAVVHSEPASRIEVDRKLWADRLRKASAIFKEFAEINDSSVTFNVEQVRRFLVN
                     SEGSRIEDNRDSLRITAEASAIAKDGMVVRLYDGIEVPRQADLPSQEKIEEMVRKLAR
                     AVVDLRNAPPAQPFVGPAILNSKAAGVFFHEVLGHRVEGHRQKDETEGRTFAQKVGEQ
                     IMPAFMSVCDDPTCTSFGAKPLVGWYRFDNEGVPAQRVQLVERGVLKGFLMGRSPIRN
                     FESSNGHGRGAPGASPVARQGNLIIDSAKRVPYGKLKAMLIQEAKAQRKPYGLIIDEI
                     AGGFTITQTFQPQSYSLLPLRVTRVYVDGRPDELTRGVNIVGTPLSSLEKIVCAARDD
                     DTFNGRCGAESGWVPVSATAPSLLVKNIELEKEYKQQDQPPILPPPAEGK"
     gene            1987..>3117
                     /locus_tag="PKW73_07095"
     CDS             1987..>3117
                     /locus_tag="PKW73_07095"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNA73086.1"
                     /translation="MDSPASAALGAASASEGERSDVVLKAMGDELARTMKSLKLPAYP
                     LPYFGSFDVTDEQSMQITASFGALSSRDVLRSRQSDITLRVGDKRLDNSSMGAGLGML
                     SQRGSLVLEDNYDALRRDLWMQADRTYKQAVESLTSAKAQLKYVNIEDRPDSFSDAKP
                     VVSIGKGVTMDADLEQWSKRIRALSSVFKEFRTIRESSVVLNVRARTKRLVNSEGTIV
                     KTGETGALVFVSAAAQSKDGMTISDGEVFAADKDSELPSQEAMESSIRRLCQRIEAMT
                     AAPRAADYQGPVLFEKQAAGELVATLLPSVVCARGDTGFGSEEEQKLGKPVLASSISV
                     LDDPIVKSYKGKTLKSGWSIDYEGVEARPLRLVEKGVLKEVCS"
BASE COUNT          684 a          737 c          945 g          751 t
ORIGIN      
        1 taatgttaag aattagaggg ggcggtggaa gctatgaccg ccgctggcac cgcccattgt
       61 ctgtcggatt tatgccgcct atgggagctg gaattttatc agttgctctc agtctttctc
      121 ttgggtttgt gggggcgatc tgggttgcac ccactgcctg cgccgaggac ggcgcggcat
      181 cagatagtgc caccgccgag cctgagaatg ctgaaagcaa gtctatcgta cttactgcca
      241 tggaagcgga gttggagcgg tccatgcgca agttgaaaaa tgctgccgcc gctccgctct
      301 actacttgag ttacagtgtc tacgatgtgc aaactttaga tcttggtgct tcctatggtg
      361 cccttggata tgacgacgat agccgcagcc ggaacctcta cgtagaactg agagtcggcg
      421 ataaaactct cgataatacc catcagttcc gctcgcaggc gccggctggt tcacccctgg
      481 catttggacg cggctattcc gggggcggta gtttcccgct ggaggatgac cgggatgcta
      541 ttcgtaccag gctctgggat gcaacggatg gtgccttcaa gcaagcccag atcgattata
      601 tgagggtaaa gaccaatcgg gatgtgcgtg tggcggagga agacgccagt agcgacttct
      661 cagcggccac tgcggtcgta catagtgagc cggcttcccg aattgaggtg gatagaaaat
      721 tatgggcgga tagattgcgt aaggcttctg ccatcttcaa agagtttgcg gagataaacg
      781 attcttcggt taccttcaat gtcgagcagg tgcggcgttt tctggtcaat tccgagggta
      841 gccgaataga agacaatcgc gatagcttgc ggatcacggc tgaagccagt gccattgcca
      901 aagacggtat ggttgttcgt ctttatgacg ggatagaggt gcccaggcaa gccgatttac
      961 ccagccagga aaagatcgag gaaatggtgc gcaagcttgc cagggccgtg gttgatctgc
     1021 gcaatgcacc gcctgcacaa ccctttgttg gtcctgccat cctcaactcc aaagcggcgg
     1081 gagtattctt tcacgaagtg ctcgggcacc gagtcgaggg gcacaggcag aaggatgaga
     1141 ctgaaggtcg taccttcgcc cagaaagtgg gtgagcagat catgcctgcc tttatgtcgg
     1201 tctgcgatga tccgacctgc acttcctttg gtgccaaacc cctggtgggt tggtatcgat
     1261 ttgacaacga gggggtgccg gcacaaaggg tgcagctggt cgaaagagga gtcttgaagg
     1321 gctttttgat ggggcgcagt ccgattcgca atttcgaaag ttcaaatggt catggtaggg
     1381 gtgctcccgg agcttctccg gtagccaggc agggcaatct gatcatcgat tcagccaagc
     1441 gagtgcctta cggcaagttg aaagcaatgc tcatccagga ggcgaaagca cagcgcaagc
     1501 cttatggcct gatcatcgat gagatagccg gcggcttcac tatcacacaa actttccaac
     1561 ctcagtctta ttcgctttta ccgttgcggg ttacgcgtgt ttatgtggat ggtcgtcccg
     1621 atgaacttac tcgcggcgtc aatatagtcg gaactccctt gagttcgttg gagaaaattg
     1681 tttgtgcagc ccgggatgac gatacattca acgggcgttg cggggccgag tcgggctggg
     1741 tgccggtttc tgccaccgct cccagtctcc tagtgaagaa catcgagctg gaaaaggaat
     1801 acaagcaaca ggatcaaccg ccaattttgc ctccacctgc tgagggtaag tagattattc
     1861 atgttttttg atagccgcta ttttagaaga agatctgttc ttaccggtgt tttggccggc
     1921 agtgtgtcat tagttgccgg tttacttgcc tcagtttcgg ttggattagc tcaggcgcca
     1981 ttggcggtgg actcgccggc gtcggctgcc ctgggagccg cctcggcaag cgagggagag
     2041 cggtcggatg tggtgctgaa ggccatgggt gatgaactgg ctcggaccat gaaaagtctc
     2101 aagttgcctg cttatccgct cccttatttt gggtcctttg atgttaccga tgagcagtct
     2161 atgcagatta ctgccagttt cggggctctt tccagccgcg atgtgttgcg cagtcggcag
     2221 tcggacatca cgctgcgagt tggtgataaa cgcctcgata actcttccat gggcgccggc
     2281 ctgggcatgc tatcccaacg gggtagtctg gtgctggaag ataattatga tgctctccgc
     2341 cgcgatttgt ggatgcaggc agatcgtact tataaacaag cggtggagtc tctcaccagc
     2401 gccaaagcgc agttgaaata tgtgaatatc gaagaccgtc cggattcctt ttctgatgcc
     2461 aagccggttg ttagcatcgg aaaaggggtg acaatggacg ccgatcttga gcaatggtcg
     2521 aagcggatca gggcgctttc cagtgttttt aaagagtttc ggacaattag agaatcgtcg
     2581 gttgtattga atgtacgggc tcgtacaaag cgccttgtga atagtgaggg gaccatcgtt
     2641 aagaccggag aaaccggggc gctggtcttt gtcagtgcag cggcgcagag caaagacggc
     2701 atgaccatct ccgacggaga agtctttgct gccgacaagg atagtgaatt gccctctcag
     2761 gaggcaatgg agtcttcaat acgccggctt tgccagcgta ttgaggccat gaccgcggca
     2821 ccccgtgccg ctgactatca ggggccggtc cttttcgaga aacaggcggc cggtgaactg
     2881 gttgctactc ttttgccgtc tgtggtttgt gctcgcggtg acaccggctt cggttccgaa
     2941 gaagaacaga aactgggaaa gccggtgctg gcaagttcga tcagcgtatt ggatgatcca
     3001 atcgtgaagt cttacaaggg taagaccttg aagagcggtt ggagtattga ttatgaaggg
     3061 gtggaggcca gacccttgag actggtcgaa aagggtgtat tgaaggaagt atgcagt
//
LOCUS       DAOQNZ010000327         3116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16036_length_3116_cov_3.473701, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000327 DAOQNZ010000000
VERSION     DAOQNZ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16036_length_3116_cov_3.473701"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKW73_07100"
     CDS             <1..692
                     /locus_tag="PKW73_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73087.1"
                     /translation="GYYPAHYHLANILVQLGEYAEARTQYQAVLSLKPDKTTAGHCQN
                     ALARLAYMPAYGEPPAKAKQLNPAVAQQSIFEDMASRAQALDANRSAQMQTERARILA
                     DADKQADQVLDDAQKRISNLSSTGNWRVYYTNEKHEPTGEAGVGVPDRVANSILEPAR
                     EEANRIREAAKQRVARMSGGGGSMADTVMGLSKQAQSESGPRLSLDSNLHVRNYSHTS
                     NMVAGKSSPTR"
     gene            complement(697..1095)
                     /locus_tag="PKW73_07105"
     CDS             complement(697..1095)
                     /locus_tag="PKW73_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73088.1"
                     /translation="MKSSIIVYAALAMPLLAAVGIPFSPSCLAQNQPGGLIPMGNTNM
                     PAGQYMMTNMNSGQSYYVLVNGTGQLLVQDPRALQFNVQPLAAATTAGATSTAQPAQP
                     NAPATPAGGFSSILKQGLDTFLQTRTQPAQ"
     gene            complement(1184..2899)
                     /locus_tag="PKW73_07110"
     CDS             complement(1184..2899)
                     /locus_tag="PKW73_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNA73089.1"
                     /translation="MSLKPNADKAKSPKGATSSGPVSLPSERKGSWVKVKGGPVVAVV
                     DMGTNSFHMIVCQASPEKDHFEVLAKVKEAVPFFRRSLSAHYIDEQALRSALRILRDM
                     LKNAREHGASYTMAVATSAVRESKNGEECLRRIRAELKMDARMISGKEEARLIYLGFL
                     FFLTQFMPHMKGRFCIVDIGGGSTELIVGDEKRIYFAESYKLGAARLTQRFFKKGEVT
                     PQTVQDLHNEVRGVLRPAASGISVAGGFDTLVGTSGTIQALAKLDRVSSGNPHAELAG
                     WTIPLVRLEKLVDYIKSFALKGEKPKGVSADRNETILAGAIVLMETMKALGAGELKVS
                     PHALREGVVVDRFLQTGWLDAGLTMHRDPRSMSVLNLLEKYQGSFEHAQQVAFLSQLL
                     FTQTRGILHNHPEEVGHLLWSASMLHDLGMFVSRNGHHKHSYYLIKNGGLLGHSEEEV
                     AIIAAIARYHRGSEPKVTHEAWYTLDLEGRKLVHDLAAFLRIAEALDRSHRQVVRDLR
                     VMVNASHRDNKVLNLLLSLHEAETAQSEIWALGEKKAFFEMHFQVSLDVLVQADALVS
                     APPND"
BASE COUNT          763 a          869 c          838 g          646 t
ORIGIN      
        1 aaggctatta tccggcccat tatcatttag ccaatatcct ggtgcagttg ggcgaatacg
       61 cggaagcaag aactcaatat caagccgtgc tatcgcttaa acccgacaaa accacggccg
      121 ggcattgcca gaacgccctg gccagactag cctatatgcc ggcctatggt gaaccaccgg
      181 ccaaggccaa gcagctcaac cctgccgtgg cgcagcaaag tatctttgaa gacatggcca
      241 gccgggccca ggccctcgat gccaaccggt ccgcgcaaat gcagacggaa agggcgcgca
      301 ttctcgccga tgccgacaag caagccgatc aagtgttgga cgacgcccag aaaagaattt
      361 ccaatctaag cagcacgggc aactggcgag tttattacac gaacgaaaag catgagccta
      421 ctggtgaagc cggtgtaggt gtacccgatc gggtggccaa ctccattttg gaaccagcca
      481 gagaagaggc caatcgcata agagaagcag ccaaacaaag agtagcgcgc atgtccggag
      541 gcggcggcag tatggccgac actgtcatgg gcttgagcaa gcaggcccag agtgagagcg
      601 gacccagact cagcctcgac tccaacctgc atgtaagaaa ctacagccac acaagcaata
      661 tggtggcggg caagagcagc cccaccagat aagcgtctat tgggcaggtt gggttctggt
      721 ctgcaagaaa gtgtccagac cttgctttaa gatgctgctg aagccacctg cgggagtggc
      781 cggcgcattg ggctgggccg gttgagcggt gctagtcgca ccagcagtag tggcagcagc
      841 caggggctgc acgttgaatt gcagggcgcg gggatcttga acaagaagct gaccagtgcc
      901 gttcaccagc acatagtaag actgccctga attcatattg gtcatcatgt actgcccggc
      961 aggcatattg gtattaccca taggaatgag tccgccgggc tgattctgag ccaggcaaga
     1021 aggggaaaat ggaataccga cagcggctaa aagtggcatg gccagggcgg cataaacaat
     1081 gatcgatgat ttcatgtgct cttcctcgaa gtagaattag tttcaacagt ttgcctgcgc
     1141 taccggactt ttgccgctag caccagatac actaccagca gactcaatca ttcggcggcg
     1201 cgctcacgag agcatcagcc tgcaccagaa catcaagact gacctgaaaa tgcatttcga
     1261 agaaggcctt cttctcgccc agagcccaga tttcagactg ggctgtttcg gcttcatgca
     1321 aagacaatag caaattcaga actttattgt cacgatggga agcattgacc atcacccgca
     1381 agtcgcgcac cacttgccgg tgtgatcgat caagggcttc ggcgatgcgc aggaaagcgg
     1441 ccaggtcatg gaccaatttg cgcccttcca ggtcgagggt gtaccaggct tcgtgggtca
     1501 ccttaggttc agagcctctg tgatagcggg cgatggccgc aataatcgcc acttcttcct
     1561 cggaatgtcc tagaagcccg ccgttcttga tcaaatagta agagtgcttg tggtgcccat
     1621 tgcgactgac aaacattccc agatcatgca gcattgaggc agaccagagc aaatgtccga
     1681 cttcttccgg gtgattgtgc aaaatgcccc tggtttgagt gaacagaagc tggctcagaa
     1741 aggctacctg ttgggcatgc tcgaagctac cctgatactt ctccaaaaga ttcagcacac
     1801 tcatggagcg aggatccctg tgcatggtca gaccggcatc cagccagcct gtctgcaaga
     1861 agcgatcgac cacaacccct tcccgcaaag catgggggga aaccttgagt tctccggcgc
     1921 ccagagcctt catggtctcc atcagcacaa tggcgccggc cagtatggtc tcgttgcgat
     1981 cggcgctcac acccttgggt ttttcaccct tgagggcaaa agacttgatg tagtccacaa
     2041 gcttttccag gcgcacaagg ggtatggtcc agccggctaa ttcggcatgg ggattgccgc
     2101 tggatactct gtccagtttg gccagagcct gaatagtgcc cgaggtcccc accagggtat
     2161 cgaagcctcc ggccacgctg atgccgctgg cagcaggacg caaaactccg cgcacctcgt
     2221 tatgcagatc ttgcacggtt tgaggggtca cctcaccttt tttgaaaaac cgctgggtaa
     2281 ggcgagccgc acccagtttg tatgactccg caaaataaat gcgcttttcg tctcccacta
     2341 taagctcggt actaccgccg cctatatcca cgatacaaaa gcgccctttc atatggggca
     2401 taaactgtgt aaggaaaaag aggaagccca gataaatcaa gcgcgcttct tctttaccgg
     2461 aaatcatgcg ggcatccatc ttcagctcgg cccgaatgcg gcgaaggcat tcctcgccat
     2521 tcttcgactc ccttaccgcc gaagttgcca ccgccatggt ataggaagcg ccatgctcac
     2581 gggcgttctt gagcatatcc cgcagtatgc gcagagccga acggagggcc tgctcatcaa
     2641 tataatgagc gctcagggac cggcggaaaa acggtactgc ctccttaact ttagccagca
     2701 cctcaaaatg gtctttttcg ggagaagcct ggcagacgat catatggaag gagttggtgc
     2761 ccatgtccac cacggccacc accgggccgc ccttcacctt tacccaggaa cctttccgtt
     2821 cggatggcag gcttaccggt cccgatgaag tggcgccttt tgggctcttg gctttatcgg
     2881 cattaggctt gagactcatt aaagctggtt cttccacagg atcttggacg ggatcttaca
     2941 ttagaatcac ggacctaata ttaatacacg ctctcggcaa gctcggccat tagttttttt
     3001 gcgcacaggt ttgcgctgtt aacacaatcc ggaatgccga cgccccggta ggaagcaccg
     3061 gtcaaataga gtccgggctg gtcggcgaca gtttcctcga tctcagccaa tagagc
//
LOCUS       DAOQNZ010000328         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16060_length_3114_cov_3.438379, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000328 DAOQNZ010000000
VERSION     DAOQNZ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16060_length_3114_cov_3.438379"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKW73_07115"
     CDS             <1..1154
                     /locus_tag="PKW73_07115"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA73090.1"
                     /translation="DLPIYIKFSGTKYPTYKTREIMNGAIDPRVFNGKVVIIGTNFSQ
                     KVDNTPRLRTPLNVKAPNIYIQAEAISTLTKNEAIRSFPRTILHHLLLLIGGALGALS
                     STLPMGRRTTAFLTFAMLLVAMTQIAFQYLNLHVPVLPLLAVLMLTYIFGTLIYLDTD
                     LRLRNKELALAREAMQVRAEEERQRIAEDLHDETLPALSAVARMADRLTHEMDGSDTP
                     RQMREKLDGAISEMRRVINDLHPSVLETMGFKPALENLLAILERETNIHTDFIDGDAD
                     PSAEYNLNDFTRLQLYRIVQESLNNVQKHSKASEVKVLIKQEDGLQIDIIDNGKGIDL
                     EQVRLTNKTSHGLVNIRQRAQLIGAQVDWKKPLEYPTGTQVIIRMPLTN"
     assembly_gap    1303..1402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1403..1777
                     /locus_tag="PKW73_07120"
     CDS             <1403..1777
                     /locus_tag="PKW73_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNA73091.1"
                     /translation="VVEAARAVLSGDCWIHPGIARVIQRTQNRATSLTAAEYEALVCI
                     ALGMTDHAIAKKLYLTEKAVQSRLKSLYAKLGIPGRSEAHDTEFNQRCRAVYVSNRRG
                     LINQSELDEWEAKLQDSKADAK"
     gene            1791..>3114
                     /locus_tag="PKW73_07125"
     CDS             1791..>3114
                     /locus_tag="PKW73_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73092.1"
                     /translation="MRARAEKQLATQAGLGALPQTLAGGRADSKRSLLCLSLSFGLSM
                     LMVMAGDVGQCAPLLASAPDQGQNPGKGGLERRDLTPYCQAEAGVKLLMGIFSRLGNE
                     PQLALKNEMQNRKADLRQEPVPASLLAGAGNISDVSQGQLSKNYTDPALAIRPQQTSV
                     RRLTVGRAVINKVVPIDGMTIAMAPRGSVNAPAPAMQALPSGAGGAAGDSLAPAAPPA
                     SFQSQAQGLGASTRNMRPSFQGALPRAEGQRYAQQAGGYSSNSDALQEKVATNKLLDL
                     KSQLNQGGGASKPVNSIPYLAGSLHSTMSPMQDKSRLKEFNSESKRTSYGGASEGLNR
                     GRRIEENLSQASYDEGRVVQTPAAEPSVNGIIRPAEQPGLFKYVREHRLELPKDAPRE
                     IASRESRLDTKDSFRESEREQSKSVGQEELSYGAVSEAGKARAVLAKQV"
BASE COUNT          811 a          861 c          785 g          557 t
ORIGIN      
        1 aagatctacc tatttatatc aagttctccg gcaccaagta tcccacctac aagacacggg
       61 aaatcatgaa cggcgccatt gatccccggg tattcaacgg caaagtagta ataataggta
      121 ccaatttcag ccagaaagtt gataacacac caagactgcg cacgccgctc aatgtgaagg
      181 cgcccaacat ctatatacaa gcggaagcaa tcagcactct gacaaaaaac gaggcgattc
      241 gctcctttcc ccgcaccatt ttgcatcact tacttttgct tataggcgga gcgctggggg
      301 ccctttcttc cactctgccc atgggacgtc gcaccacagc ctttcttact tttgccatgc
      361 tgcttgtggc catgacccaa atcgcctttc agtatctgaa cttacatgtg ccggtgctgc
      421 ctcttctggc ggtactgatg ctcacctata tcttcggcac tctcatctat cttgataccg
      481 atttgcgcct gcgcaacaaa gaactggctc tggcccggga agccatgcag gtgcgcgccg
      541 aagaagaaag acagcgcatc gccgaagatc tgcacgatga aaccctacct gcactgtcgg
      601 cagtggcacg gatggccgac cgcctcaccc atgaaatgga cggcagcgac acaccgaggc
      661 agatgcgcga gaaattggac ggcgccattt ccgaaatgcg ccgggtgata aatgatttac
      721 acccatcggt tttggaaacc atgggcttca agcccgccct tgagaatctg ctggccattc
      781 tggagagaga aaccaatatc cataccgact tcattgacgg cgacgccgat ccctcggccg
      841 agtacaactt aaacgacttc acccgcctgc agctctaccg aatcgtgcag gaatccctca
      901 ataatgtaca aaagcactcc aaagccagtg aggttaaagt cttaattaag caagaggacg
      961 gcttgcaaat tgatatcatc gacaacggta aggggataga cttggagcag gtgcgcctta
     1021 caaacaagac atcccatggt ctggtcaata ttcgtcagcg tgcacaactc ataggtgccc
     1081 aggtagactg gaagaagccg ctagaatatc caacaggcac acaggtcata atcagaatgc
     1141 ccctgacaaa ttagagtcaa ctactgacgg aaaagccgcc aaccccaagg aaagtaaagg
     1201 tcttaaaagc atgagtatcc ttatcgtcga tgatgaagaa agagacagaa cctggctcaa
     1261 gaatctaatc accaacaatc tttccggcga attgcccctg acnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nngtagtgga agcagcccgg gccgtattat cgggagattg
     1441 ctggatccat cccggtatcg cccgcgtcat ccagcgcacc cagaaccgcg ccacctcttt
     1501 gacggccgcc gaatacgaag ccctggtctg catagcactg ggcatgactg accacgctat
     1561 tgccaaaaag ctctatctga ctgaaaaagc cgtgcaatca agactgaagt cgctctacgc
     1621 caaactgggc attcctggtc gcagcgaagc ccacgatacc gaattcaatc agcgctgccg
     1681 tgccgtctat gtttccaatc ggcgcggtct gatcaaccaa tcggaactgg atgaatggga
     1741 agccaaactg caagactcga aagcagacgc caaataaaga ggacacaaaa gtgagagccc
     1801 gggctgaaaa acaattagcg acccaagcgg gtctgggggc actgcctcag actctcgcgg
     1861 gaggaagggc ggattccaaa cggtccttgc tctgtctcag tttgagcttt ggtctttcca
     1921 tgctaatggt catggccggt gatgtgggac agtgtgcgcc gcttctagcc tcggctcccg
     1981 atcagggaca gaaccccggc aaaggcggtc tggagagacg cgacctgacg ccctactgcc
     2041 aggcggaagc cggcgtcaaa ctgctcatgg gtatattcag ccgactgggt aatgaaccgc
     2101 aactggcgct caaaaacgaa atgcaaaatc gcaaggccga cctcaggcaa gagcctgtac
     2161 cggcaagctt gctggccggc gccggcaaca tatcggacgt atcacagggt cagctttcca
     2221 aaaactatac cgatccggct ctggcgatca gaccacagca aacatccgtg cggcgattga
     2281 cggtcgggcg agcggtaatc aataaagtag taccgataga cggcatgact atcgccatgg
     2341 ccccccgcgg tagtgtcaat gcaccggctc cggccatgca agccctgcct tccggagcag
     2401 gcggtgctgc cggcgatagc ttggctccgg ccgcaccacc agctagtttc cagagtcagg
     2461 cccaggggct gggcgcctcc acgagaaata tgcgtcccag ctttcagggt gccctgccca
     2521 gagcggaagg acagcgctat gcgcaacaag ccgggggcta ctcaagcaac agcgatgccc
     2581 tgcaagagaa agtagcaaca aacaaactcc tcgatttgaa gagccaactc aatcaaggag
     2641 gcggcgcctc caagccggta aattccattc cctacctggc cggcagcctg cacagcacca
     2701 tgagccccat gcaggataaa agcaggctga aggaattcaa cagcgaaagc aaacgcacct
     2761 cctatggtgg cgcctccgag ggtctgaacc gcggcaggcg aattgaagaa aacctcagcc
     2821 aggcgagcta cgatgaagga cgggtggtgc agactccggc ggcagaacct tccgtgaacg
     2881 gcattatcag gccggccgaa cagcccggtc tgttcaaata tgttagagaa cacaggctcg
     2941 aactgcccaa agatgccccc cgggaaatag ccagtcgaga aagtcgcctc gatacaaaag
     3001 acagtttcag agaatcggag cgggagcaaa gcaaatccgt cggacaagaa gaactaagtt
     3061 acggtgcagt atcggaggcc ggtaaggcca gggccgtcct tgctaagcag gtgc
//
LOCUS       DAOQNZ010000329         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16263_length_3093_cov_3.512508, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000329 DAOQNZ010000000
VERSION     DAOQNZ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16263_length_3093_cov_3.512508"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1189
                     /locus_tag="PKW73_07130"
     CDS             161..1189
                     /locus_tag="PKW73_07130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNA73093.1"
                     /translation="MKVLCLGKLPPPLQERLKTEAKIESVTYVDRAAPLPDPELTAVL
                     SGIDIVLSEPQDNLSENILSRCQALKMVAQRAVGYDNVDLDYCNRSGILLSNTPGVLD
                     NATADLAFSLLLCAARRIVEADRYVRAGLWHGFESDLLLGREISGKTLGIVGLGRIGE
                     AMARRAWGFGLKIIYCRSDHKNSGTTEFELDEKDRRLAQSLGARRVSMEDLLRESDFI
                     SLHCPLKKETTNLIGAAELALMKNGCILVNTARGKVVEEKALVEAVTKGKIIAALDVF
                     AQEPTVPPPLLNSPNTVLAPHIGSATDETRFAMAELAVNAVLQVLQGGLPANLVNPQI
                     FESTVNKL"
     gene            1186..2484
                     /locus_tag="PKW73_07135"
     CDS             1186..2484
                     /locus_tag="PKW73_07135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNA73094.1"
                     /translation="MTAKFSLSEKNRTRLWLIYFAVLFFWGLNACFFLGKTYAKGEMF
                     AYEIDKHPYVSDFINSYSAGVLCSRCLKDRDNPPDIYNSKVQADLQNELIAPIKPENP
                     FYNQYPPVMFLFFLPLSLLSMGQAWIAYCVFAFLLNAALLYYLVLPAYKTVQEKVFLI
                     TAIFSSYPFWFAFRLGQPALLLMPTILVFFHCLKNGNMLLAGLTSSLVLLKLQYLPVV
                     VAIGFGAGRLRYLAGFTGGALVLMLLSGLAVGFKNLVNYPLALFSGEAGRSTTGVAPE
                     MMQNLRGCLVLLSGQDSRAIHITAVAVCLTTALALFIIWFQYTRKKDQSKEPDKFNLL
                     SAASLLTMLITSVHTHIQDYVLAVRAFEMTYQANRSHKTFLTYLALFFPLISWPLAML
                     LQFIQIAKIQPYAIYALIAVIALVLEMKKRKAAKETAPSA"
     gene            complement(2537..>3093)
                     /locus_tag="PKW73_07140"
     CDS             complement(2537..>3093)
                     /locus_tag="PKW73_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73095.1"
                     /translation="ASELYRLRLRGDPPTTLTWVADTPGFNLYDFLHPEPKDVAFQFP
                     EIEALKQSCRFTDCLHLVESGCAVLLALQKSEDEDGEAPALPDGPSPSETISLSRYAS
                     YVTLVQESLARKEESRSVSKKVESTVKSVGGEAKAKSIPRLNQRYRFTSRRKEKQDLR
                     NICKTDKERQGGDQAGDESEGDHD"
BASE COUNT          703 a          893 c          767 g          730 t
ORIGIN      
        1 gcaccataag cagtgcctgc aggcagcgcc caaagtagcc aggatggtgg ccgaaacccg
       61 aggctgggat aacgctcgta tagccagtga aatagccaat ctggaactaa atctcggccc
      121 gcaattggcg ccggcgctct aatcgatacg gtatgaaaaa gtgaaagtac tctgcctggg
      181 caagctccct ccaccactgc aagaaagact caagactgaa gcgaagattg agtcggtcac
      241 ttacgtggat agggccgccc cgctgcctga tcctgagctt acggcggttt tgagcggcat
      301 agatattgtg ctttcagaac cccaggacaa tctaagtgag aacatattga gccgctgcca
      361 ggcactgaaa atggtggcac aaagagcggt tggctacgat aatgtcgatc tggactattg
      421 caatcgttcc ggcatcttgc tgtcaaacac gccgggcgtt ctagacaacg ccactgccga
      481 tctggctttc agtttgctct tgtgcgccgc cagaagaatc gtcgaggccg atcgctatgt
      541 gagagcaggg ctctggcatg gctttgaatc agacctgctc ttaggccgtg agataagcgg
      601 caagacattg ggtatagtcg gtctcgggcg cataggtgaa gccatggcca gaagagcctg
      661 gggcttcgga ctgaaaataa tctattgccg ctccgaccac aagaattccg gtaccactga
      721 attcgagctg gatgaaaaag accggcgcct ggcccagagc ctgggcgccc ggcgagtctc
      781 catggaagat ttgctcaggg aaagcgactt catctctttg cactgcccgc tcaagaagga
      841 aaccacaaac ctcattggag cagccgaatt ggcccttatg aaaaacggct gcatcctggt
      901 gaacacggct cgcggtaaag tggtggaaga aaaagccctg gtagaagcgg tcaccaaagg
      961 caagatcata gccgccctcg atgtcttcgc ccaggaaccc accgtgccgc cgccactatt
     1021 gaactcacca aatacggtgc tggcgccgca catcggttcg gccaccgacg aaacacgttt
     1081 tgccatggcc gaactggccg tgaacgccgt actgcaagta ttgcagggcg ggttaccggc
     1141 caacctggtc aatcctcaga tattcgaaag cacggtcaat aaactatgac ggcaaaattc
     1201 agcctcagcg aaaaaaaccg caccagacta tggctcattt acttcgccgt cttgtttttc
     1261 tggggtctta acgcttgctt tttcctgggc aaaacctatg caaagggcga aatgttcgct
     1321 tatgaaatcg acaagcaccc ttatgtaagc gacttcatca attcttacag cgccggtgtg
     1381 ctctgtagcc gctgcctcaa agatagagac aatcccccgg atatctataa ctccaaagtg
     1441 caggccgact tacaaaatga gcttatcgct cccattaagc cggagaaccc tttctacaat
     1501 cagtacccgc cggtgatgtt tctcttcttc ctgcccctgt cgcttttatc tatgggtcag
     1561 gcctggatag cttattgcgt ctttgccttc ctattaaatg ccgccttgct ttactatctg
     1621 gtcttaccgg cttataagac cgtccaggaa aaagtcttcc tcattacggc tattttttcc
     1681 tcctatcctt tctggttcgc cttccgcctg ggacaaccgg ctcttttgct gatgcctacc
     1741 atcctggtct tcttccactg cctcaagaac ggcaacatgc tactagcggg tcttacctcc
     1801 agcctggttt tactgaagct gcaatatcta cctgtggtgg tggccatcgg ctttggtgcc
     1861 gggcgcctgc gctatctggc cggcttcacc ggcggtgccc tggtactgat gctcttatca
     1921 ggtctagccg tgggcttcaa gaacctggtc aactaccccc tggcgctttt ctcgggcgaa
     1981 gcgggtcgct ccaccaccgg cgtcgccccg gagatgatgc aaaacctgcg cggctgcctg
     2041 gtcttgctct caggacagga tagccgagcc attcatataa cggccgttgc agtctgcctg
     2101 accacggccc ttgccctgtt cataatctgg tttcaataca caaggaagaa agaccagtca
     2161 aaagaaccgg ataaattcaa cctgctctcg gctgccagcc tgcttaccat gctcatcacc
     2221 agcgtgcaca cccatattca agactatgtt ctggctgtgc gcgcctttga aatgacctac
     2281 caggcaaacc gcagccacaa aacctttctt acttatctgg ctctcttttt cccgctcata
     2341 agctggccgc tagccatgct cctgcaattc attcaaattg ccaaaattca gccctatgcc
     2401 atctacgctc tcattgccgt tattgccctg gtcttggaaa tgaagaagcg aaaggcggca
     2461 aaagaaacag cgccttcagc ctgatcaggt taaaagacca gctctagttc tgttctagtt
     2521 ccgttctagt tccgttctaa tcatgatcgc cttcgctctc atcgccagcc tggtccccgc
     2581 cttgccgctc tttgtcggtc ttacagatat tgcgcaggtc ctgtttctct ttgcgtctgg
     2641 aagtgaagcg atagcgctgg ttgagcctgg gaatgctctt agccttagcc tcgccgccca
     2701 ccgatttgac cgtagactcc accttcttcg atacgctgcg gctttcttcc ttgcgagcaa
     2761 gcgattcctg caccagtgtc acatagctgg cataccggct caagctgatg gtctcgctcg
     2821 gcgaaggccc atccggcaaa gcgggcgcct cgccatcctc atcctcactc ttttgcagag
     2881 caagaagcac cgcgcagccg ctttccacca ggtgcagaca atcagtaaag cgacaactct
     2941 gtttgagcgc ttcaatctcg ggaaattgaa aggcaacgtc cttgggctcg ggatgcagaa
     3001 agtcatatag gttgaaaccg ggagtgtcgg ccacccaggt caaagtggtg ggcgggtcgc
     3061 ctctcagacg cagacgatac agttcactgg cgg
//
LOCUS       DAOQNZ010000330         3078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16423_length_3078_cov_2.980483, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000330 DAOQNZ010000000
VERSION     DAOQNZ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16423_length_3078_cov_2.980483"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="PKW73_07145"
     CDS             <1..1458
                     /locus_tag="PKW73_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNA73096.1"
                     /translation="VFPASLVAFILTGICLDRLIFPLLEDHKIFWGAAWSDDEKKLFK
                     LNCLRNWASSQDPLFRNHLIQPLAEKERRGPRILVMGDSFVWGDGYRNINDLWWRQLS
                     RQLSDRGFSNVEVLAAGYPGFSTALETKAARRLIRSLKPDLIVFGYTENDADEGLVKQ
                     NAAASISRASGLFKRILPNLCIYIDRQIFSRWKSFSSLETANEDRKDQQWEFQLLDGE
                     NFKRFKGTVADMGQLLKESGTPGFVVSLPKSPDAQYYEACFEPVKPLFSEAAVPFLDL
                     AQPLKGWYGEALPTVLALNPLALVNPGSFLQISPANSHPSAALTYFYGRQAADFIEKN
                     YRHILGAKQPNASAPALSINDCMPIFAGGNRTGSGVEFAYPDPSLLLKMPGRKPFVQF
                     NLENPLPLKKILVKPAGKLPTACTFYVTTINAAVGFDTGKTMIFEGRPGAAGQFEVDL
                     SAVKEAVNTVGISANYAGAPGAKAAERMLQLELAR"
     gene            complement(1460..2281)
                     /locus_tag="PKW73_07150"
     CDS             complement(1460..2281)
                     /locus_tag="PKW73_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNA73097.1"
                     /translation="MEGMLGFLVFLLAYTVSALKVIKEYEKIVVFTLGRADGVKGPGL
                     QFVWPIFQQSKKVDLRIMTMPIPMQEIITKDNVSVKTAAVCFFQVDDAFKAVTKIEDP
                     LAATMQIAQTTLRSILGQHELDELLTERDAINAKLSAIIDRQTEGWGIKVSAVEVKDV
                     EIPVSMQRAMAKQAEAERERRAKIVAAEGEHQAAEKLAQAAEVISGTPGAMQLRQLQT
                     MVEVSAEKNSTLIFPIPIEFMDLAKGASKLLNRQEESQPGETIKIKEKAAELDKA"
     gene            complement(2320..>3078)
                     /locus_tag="PKW73_07155"
     CDS             complement(2320..>3078)
                     /locus_tag="PKW73_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA73098.1"
                     /translation="SRGKDRPKLEEFPGFAEGIFTVQDEAAAFVSQVVQAAPGETVID
                     LCAAPGGKTMNMAEHMVGKGRIIAVDIHDSRLKLLRETRQRLGLRNIETRQADGRFFK
                     FEGGADRVLVDAPCSGTGVIAKRSDLRLNRLESDLEKLTRLQTALLNNAAKLVRPGGL
                     LVYSTCSIEKEEGPQIVAQFLAEHSDFQLEDLSEAFPPDLVNKWNLAEEAHSGQVMFL
                     PSRHAPQGFYIARLRKSADLPEAEHEQEPGGDII"
BASE COUNT          610 a          806 c          831 g          831 t
ORIGIN      
        1 gtgtttcctg ccagtctggt ggcctttatt ttgaccggga tttgtctcga taggcttatc
       61 tttccgcttc tggaagatca caaaatattc tggggagcag cctggtctga tgacgagaag
      121 aaacttttca agctcaattg tctgaggaac tgggcgtcca gtcaggaccc acttttccgt
      181 aatcatttga ttcagcccct ggcggagaaa gaacgtcgcg gaccgcgcat tctggtcatg
      241 ggtgattctt tcgtctgggg cgatggttat cgaaacatca acgatctgtg gtggcggcag
      301 ctcagtcggc agctttcgga ccgcggtttc agtaatgtgg aagtgctggc ggctggttat
      361 cccggctttt ctacggccct ggaaacgaag gcggcgcgcc ggttaattag aagtctcaaa
      421 ccagatttga tcgtttttgg ttatacggaa aacgatgctg atgagggtct tgtgaagcag
      481 aatgccgccg cctccatctc acgagcttcc ggcttgttca agcgtatctt gccgaatctc
      541 tgtatttata ttgaccgaca gatcttctct cgctggaaga gcttttccag tctggagacc
      601 gccaacgagg acaggaaaga tcaacagtgg gagtttcaac ttctagacgg tgagaatttt
      661 aagcgcttca aaggcaccgt ggccgacatg ggtcagcttt tgaaagaaag cggtacacct
      721 ggttttgtcg tcagtctgcc taagtccccg gatgctcaat attatgaggc ttgtttcgag
      781 ccggttaaac cgctcttctc ggaagcagcc gtgcctttcc tcgatcttgc tcagcctctg
      841 aaaggttggt acggggaagc tttgccaacg gtgcttgccc ttaatcccct ggctctggtc
      901 aatccgggta gtttcctgca gattagccct gccaattccc atcccagtgc tgcccttact
      961 tatttttacg ggcggcaggc ggctgatttt attgaaaaga attaccggca tatcttaggt
     1021 gcgaagcaac caaatgcttc tgctccggct ctctccatca atgattgcat gccgattttc
     1081 gccggcggta accgcaccgg ttccggtgtg gaattcgctt atcctgatcc ttccctgctt
     1141 ctaaagatgc caggacgcaa gccctttgtg cagttcaatc tggaaaatcc actgcctttg
     1201 aagaagattc tagtaaagcc ggcaggcaag ttacctaccg cctgcacttt ctatgtcact
     1261 actattaatg ccgctgtggg cttcgacacc ggcaagacca tgatctttga aggaagacca
     1321 ggggccgccg ggcagtttga agtggacttg agtgccgtca aggaagccgt caatacggtg
     1381 ggcattagtg ccaattatgc cggagcaccg ggcgctaaag cggcagagcg gatgctgcaa
     1441 ttggaacttg ccaggtaagt caggctttgt ccagttccgc cgctttttct ttgattttga
     1501 tggtctcccc aggttggctc tcttcctggc ggttcaagag tttggatgcg cctttggcca
     1561 ggtccatgaa ctctattgga atggggaaga tcaaggtgga gtttttctcg gcagagactt
     1621 ctaccatggt ttgcagttgt ctcagttgca tggcgcccgg tgtgcctgag ataacttccg
     1681 ctgcctgagc cagtttctcc gcggcctggt gttcaccttc ggcggctacg attttggctc
     1741 ttctttcccg ctccgcttcc gcctgcttgg ccatggctct ttgcatggaa acaggaattt
     1801 ccacatcttt cacttctacg gcgctgactt taatgcccca gccttccgtc tgtctgtcga
     1861 tgatggccga gagcttggca ttgatggcat ccctctctgt taaaagctca tctaattcgt
     1921 gttgaccgag gatgctgcgc agcgtggtct gggcaatctg catggtggca gccagcggat
     1981 cttcgatttt ggttacggct ttgaaggcgt catctacctg gaagaagcag acggctgcgg
     2041 ttttgacgga gacgttgtct ttggtgatga tttcctgcat gggaataggc atagtcataa
     2101 tgcgcaggtc gactttttta gattgctgaa agatgggcca gacaaactgc agccccggac
     2161 ctttgacgcc gtctgcacgg ccgagggtga agacaactat cttttcgtac tctttgatta
     2221 ccttcagagc gctgacggtg taggccaaca ggaaaaccag gaaacccagc atgccttcca
     2281 tattatttac cttccttaga tttgctcccg caactttctc tagattatat cgcccccggg
     2341 ctcttgctca tgctcggctt cggggaggtc ggccgacttt cgcagtctgg caatgtaaaa
     2401 gccctgcggg gcatgtctgg agggcaggaa catcacctgg ccgctgtggg cttcctctgc
     2461 cagattccac ttgttaacca ggtctggagg gaaggcctct gaaaggtctt ccaactggaa
     2521 gtcgctatgc tctgccagga actgcgccac aatctgcgga ccttcttcct tctcgatgga
     2581 gcaggtggaa tagaccagta agccgcccgg tctaaccagt ttggcagcgt tattgaggag
     2641 cgcggtctgc aacctggtca gtttctccag atccgattcg aggcggttca gtctcaagtc
     2701 ggaacgcttg gcgatgacgc cggtgccgga gcacggcgca tccaccagca ctctgtcggc
     2761 tcctccttcg aacttgaaga agcgaccgtc tgcctgtctg gtttctatat tgcgaagacc
     2821 cagtctctgt ctggtttcac gcaagagctt cagtctgctg tcgtgaatgt ctacggcaat
     2881 gatgcgccct tttcctacca tatgttcggc catattcatt gtcttgccgc ccggtgcggc
     2941 gcagagatcg atgacggttt caccgggggc tgcttgcacc acctgggaaa caaaggcggc
     3001 tgcttcgtct tgcacggtga agataccttc ggcgaagcct ggaaattctt ccagcttggg
     3061 tctgtccttg cccctgct
//
LOCUS       DAOQNZ010000331         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16433_length_3077_cov_3.650893, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000331 DAOQNZ010000000
VERSION     DAOQNZ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16433_length_3077_cov_3.650893"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PKW73_07160"
     CDS             <1..440
                     /locus_tag="PKW73_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73099.1"
                     /translation="RSHRPIQDEQMSEVSHSIAGNWFGKYGYEDETEGATFEAVFLES
                     RGYIEGSMLDDCALGEAFVSGTFVYPTISFTKKYRNARLETIEYRGAMSEDGQSLLGV
                     WWIKAELPGHIDTRGTWTAFREGSDSALPIRALRLELVAAGGK"
     gene            535..858
                     /locus_tag="PKW73_07165"
     CDS             535..858
                     /locus_tag="PKW73_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM14 family protein"
                     /protein_id="HNA73100.1"
                     /translation="MLEVAKISVLALAIIVFAGGIMGFVKAKSKASLISGVISGIALA
                     ACFAISLNEIRQGLMGALVVSGLLTVVFIIRYIKTKKLMPAGLMLLCCIGSLGLLAKA
                     LFDPN"
     gene            931..2442
                     /locus_tag="PKW73_07170"
     CDS             931..2442
                     /locus_tag="PKW73_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA73101.1"
                     /translation="MKLVHKGIALALIPLLFELSLIATLAWQLNTLEKERQLAGQGRD
                     MISLLTNWSRSMADAAIYLIVSRYHESPSEEKRFKDTLQAMEQQAKALAGSAEALPEL
                     KADIAALKARSDKLAAQLSYQLRPGDSLGGNTIFSSNLDMRNQLEPQIVQFIRDCDRL
                     SNRVKTLTHAENPVSLEHIWQWMAANGIGSLLISLVMVWFFFGSIARRILKITEDTKY
                     FKTNRKLPPIQANRGGQDEIYELEREFQDMARAVQEAGARREQFTSMISHDLRSPLAA
                     INITLAMVQTGAYGNLNAVGQERINSAEKSIQRLLNLINQLLDTEKLEAGLFQLQISR
                     VGLLSVVYPAVEALKQLADEKQIELSFRCQDYEVEVDLERMSQVVQNLLSNAIKFTPK
                     WGKITLKSEETEDSYIVSVSDTGVGIKEEFLERIFDRFEQISEHDMPRQAIRQGQAKN
                     RLSTADRLAGSGLGLSICKALVEAHGGEIGVHSLEGKGSTFWFSLPKLEPGKR"
     gene            2439..>3077
                     /locus_tag="PKW73_07175"
     CDS             2439..>3077
                     /locus_tag="PKW73_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73102.1"
                     /translation="MSKDKITIGSPECDRKRGELRKGERSTHTERSTHTESSTHTECR
                     RWGKVLLLLATLSGSGAAQARVDDISSMKALANYAQAGKFVDLHEPSKQLHYLKLALK
                     EAPDSAPLLTDLASAYFELKQPGTAKNYLEKALRAAPQCREALSRRAFIALLSNNLKP
                     AIADLEKCLAVNKIEPQYNDEGSDAANLLTASKHRTACTEAKQQQLEKHYGGL"
BASE COUNT          829 a          791 c          824 g          633 t
ORIGIN      
        1 ggcgaagtca tcgaccaatt caagatgagc aaatgagcga agtttcccat agcattgccg
       61 gcaactggtt cggtaagtac ggttatgaag acgagacgga aggcgccacc ttcgaagccg
      121 tatttctgga gagccgcggc tatattgaag gcagcatgct ggatgattgt gccctgggcg
      181 aggcctttgt ttcaggcacc ttcgtctatc ccaccatcag cttcaccaaa aagtatcgca
      241 acgcccgcct ggagaccatt gaataccggg gagccatgag cgaagacggg cagtcccttc
      301 tgggggtctg gtggatcaag gcggaattgc caggacacat agacactcgc ggcacctgga
      361 cggccttccg cgaaggttcg gacagcgcct tgcccattcg cgccctcagg ctggagcttg
      421 tggcagccgg cggcaaataa agtagaataa gcgagttcgc tcgtaagcta aatagagcca
      481 ggtggtgaaa gatgcccgat tgaaggcaga cctggcgata agaaggtaaa gtaagtgctg
      541 gaagtcgcaa aaatatccgt cctcgccctg gcaattatcg tctttgccgg cggcatcatg
      601 ggctttgtca aggccaagag caaagcttcc cttatctccg gcgtcataag cggcatagcc
      661 ctggctgcct gtttcgcaat ttccctcaat gaaatcagac agggtctgat gggtgccctt
      721 gtggtgtccg ggctcctgac cgtggttttc ataattcgct atatcaaaac caaaaagcta
      781 atgccggcag gtctgatgct cctgtgttgt atcggctcac tggggcttct ggccaaagca
      841 ctctttgatc ctaactgagc gggcgacagt gccgtttctc tcaagcggca cgagtgtcag
      901 aaagtatgca atttctaaaa ggtcaagaag atgaaacttg tccacaaagg catagcactg
      961 gccctcattc ccttactgtt tgaactttcc ttgattgcca ccctggcctg gcagttgaac
     1021 accctggaga aagagcggca attggccggc cagggccggg acatgatctc gcttctgacc
     1081 aactggtctc gctcgatggc ggatgcggca atctacttga ttgtctcgcg ctatcacgaa
     1141 agccccagcg aagagaaacg cttcaaagat acactgcaag ccatggagca gcaggccaaa
     1201 gcgctggccg gtagtgccga agccctgccc gagttgaaag ccgacatagc agcgctaaaa
     1261 gcccgcagcg ataagttagc ggcccagttg tcttaccagc tgaggcccgg cgatagcctg
     1321 ggcggcaaca cgattttctc cagcaatctg gatatgcgca atcaactgga gccccagata
     1381 gttcaattca tccgagactg cgatcgctta tcaaaccggg tgaaaaccct gacccacgct
     1441 gagaaccccg tcagcctgga gcatatctgg caatggatgg cggcaaatgg tataggcagt
     1501 ttgctgataa gcttggttat ggtctggttt ttcttcggca gtattgccag acgaattctc
     1561 aaaataaccg aagacacaaa gtatttcaaa accaatcgca agcttccccc catacaagca
     1621 aaccgtggcg ggcaggacga aatttacgaa ttggagaggg aatttcagga tatggccaga
     1681 gccgtgcaag aagccggagc cagaagagaa cagtttactt ctatgatttc ccacgattta
     1741 cgtagcccgc tggcggcaat caatatcacc ctggccatgg ttcaaaccgg tgcttacggc
     1801 aatctcaacg ccgtcggaca ggagcgcatc aacagtgccg aaaagagcat tcaaaggctg
     1861 ctcaatttga tcaatcagtt actcgatacg gagaaactgg aagcaggact ctttcaatta
     1921 caaatcagcc gagtgggact tttgtcggtg gtctatcctg ccgtggaagc cttgaagcaa
     1981 ctggctgatg agaagcaaat cgaacttagc tttcgctgtc aagactatga agtggaagta
     2041 gatcttgaac gaatgagtca ggtagtgcaa aaccttttga gcaacgccat caagttcaca
     2101 ccaaagtggg gcaagatcac acttaaaagt gaggaaaccg aggatagcta tattgtctcc
     2161 gtcagtgata ccggtgtggg aatcaaagaa gaatttctag agcgcatctt cgatcgcttt
     2221 gaacagatca gtgaacatga catgccgcgc caggcaatta gacaaggaca agccaagaat
     2281 cgactcagca ctgccgacag actggctgga agcgggctgg gcctctctat ttgcaaagcc
     2341 ctggtggagg cccatggtgg tgaaatcggg gtgcacagtc tggaaggtaa aggttcgaca
     2401 ttctggttca gcctgccgaa actggagccc ggaaaacgtt gagcaaggac aaaatcacta
     2461 tcggcagccc ggaatgcgat cgaaagaggg gcgaattaag gaagggcgaa cgaagcactc
     2521 atacggaaag aagcacccat accgaaagca gcacccatac cgaatgcagg cgctggggca
     2581 aggtgctgct gcttctcgct accttaagtg gcagcggcgc agcccaggcg agagtcgatg
     2641 acatttcctc catgaaagcg ctcgccaatt atgcccaggc cggaaaattt gtggacttgc
     2701 atgagcccag caagcaactg cactatctta aactagctct aaaagaagca ccggattcag
     2761 caccgctact cacggacctg gcctcagcct attttgaact gaagcagcca ggtactgcta
     2821 aaaactatct ggaaaaggcc ttgagggccg cgccgcaatg ccgggaagcc ctcagtcgca
     2881 gagccttcat tgcacttctc agcaacaacc tcaagccggc catagccgat cttgagaagt
     2941 gcctggcggt gaataaaatt gagcctcaat ataatgacga aggcagtgat gccgccaacc
     3001 ttcttacagc cagtaagcat cgaacagcct gcaccgaagc aaaacaacag cagctggaaa
     3061 agcattatgg cgggctt
//
LOCUS       DAOQNZ010000332         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16435_length_3077_cov_3.254798, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000332 DAOQNZ010000000
VERSION     DAOQNZ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16435_length_3077_cov_3.254798"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKW73_07180"
     CDS             <1..288
                     /locus_tag="PKW73_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73103.1"
                     /translation="MYSSGAGYENGKRIWYIEHNSERGLEDLITEGRLPACFDEIYST
                     RLGLLKSGADCGNTLMGVPVAVIQTFIGINCSHDCWPGEMGFPVYVPLEDA"
     gene            354..1139
                     /locus_tag="PKW73_07185"
     CDS             354..1139
                     /locus_tag="PKW73_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73104.1"
                     /translation="MSPPVTVPSRYFRMVFPRKDFVLECPALRECAMKITPFALLLAL
                     SAVLPLQSAFALDSDLTNLSQLSAYQMRAGAQQRRLPPTVLDSFVYNAGANAENIYGD
                     EGVNGLPPIDNFKKCNRIDAGITGIRDLGLTTGHGSVMPDAWGADEFISVGEWTQSGA
                     KGNMANPVSISATNLDSRVSNRGGSGGSIGSDENIPPPPGPGYQPVYTHGVFDGYLTP
                     EEADLWNNGNPAAAWKSFANRRPGGPTENDIFIITREMGGTYP"
     gene            complement(1398..1985)
                     /locus_tag="PKW73_07190"
     CDS             complement(1398..1985)
                     /locus_tag="PKW73_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73105.1"
                     /translation="MKRSILLPLLMAPLFLTGAVSPLPVLAQNAQFDLSSFDGGSFNG
                     GASSGSWPQNYSNQGFTPATQATLPNNYLPQAANAILSTGRTMLPVVSTGSVDFNTVD
                     LPFIRSNGGQGAFSGGPGGGGGTGNGSSQVPPDLPPGYAAVMCHGIYAGAIPPGGNIV
                     DFWNGAYGFAGDAQQQAALLEEGKWLRENGQLDGL"
     gene            2178..>3077
                     /locus_tag="PKW73_07195"
     CDS             2178..>3077
                     /locus_tag="PKW73_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNA73106.1"
                     /translation="MAVSLKLSQKGAILVAVPLFFELLFVFILVMLLQQADAEAQQQA
                     RSRAIIYQADDLSRIFHEANLTLLGYSSTRSPLFYDRFEELTGKIPAEIKELKELLGG
                     SEESRSELLADTQKGLDALCNARKFLVENSGNLADFSARHMFSAIASSTNELETKLKA
                     FIHEQESKQHEHEVAQMLARTRLKWLIAVGVLFNILISLALTVFFVRGIGRRVDVLRD
                     NAYRLAAGQKLNAPAFGDDEISELDGVFHQMARSLREAAQRESAIFENAMDVICSIDQ
                     NGRFATVSPASLKLLGFDPEDLIG"
BASE COUNT          697 a          765 c          866 g          749 t
ORIGIN      
        1 atgtatagtt ccggagccgg ttacgaaaac ggtaagcgta tctggtacat cgagcacaac
       61 agcgagcggg gcttggaaga tctcataacg gaaggacggc tacctgcctg cttcgatgag
      121 atatacagca cgaggctggg tctactcaag tcaggcgccg attgcggcaa taccttgatg
      181 ggcgttccgg ttgcagtgat ccaaacattt attggtatca actgctccca tgattgttgg
      241 ccgggtgaga tgggttttcc tgtttatgtg cctttagaag atgcttagtg ctcgccaggt
      301 tgtcgagccg ctgaatttac catagtgcgg cgctaaagct tgcagctact gcagtgtctc
      361 cacctgtcac cgtgccgtca cgttattttc gtatggtctt tccacgcaaa gacttcgtgc
      421 ttgaatgccc cgccttacga gagtgcgcca tgaaaataac accgtttgct ttactattag
      481 ctctttctgc tgttcttccc ctgcagagtg ccttcgccct agatagtgac cttaccaacc
      541 tttctcagtt aagcgcttat caaatgcgtg cgggtgctca acagcggcgc ctgcctccga
      601 ccgttctgga cagctttgtc tacaatgccg gtgccaatgc ggaaaacatc tatggtgatg
      661 aaggcgtcaa tggtctaccg cccatcgata acttcaaaaa gtgcaataga atcgacgccg
      721 gcataacggg cattcgagat ctcgggctca ctaccggtca cggcagcgtc atgcccgatg
      781 cctggggggc agacgaattt atcagtgtcg gagagtggac tcaatcaggg gccaagggca
      841 atatggctaa tcccgtttcg atttcagcta ccaatctaga ttctagagtc agtaaccgtg
      901 gcggcagtgg tggcagtatc ggttccgatg aaaatatccc gccgccgccc ggacccggct
      961 atcagccggt ctatacccat ggtgtctttg acggctacct gactcctgaa gaagctgact
     1021 tgtggaataa cggcaaccct gctgctgcct ggaagagttt tgccaacagg cgtcccggtg
     1081 gacccaccga gaacgacatc ttcattatta ccagggagat gggtggaacc tatccctagg
     1141 tccgacaaga ttggaagtga agtgcgtgtt gatagtgagg acttgagctg aggccgggtc
     1201 ctcattctat ttcctgacct ggggactttt agcggtcttg aatgagattg aaaagtctct
     1261 gcttcgaaga ggcgttggtc gcgtctggat tggctaccat cccgacgatt tgcagtgcaa
     1321 cctggcctga agagtaaaag caccgccagg gcggtgcttt ttaaaaggta ttcggaaact
     1381 ggtgcggtgg tcccgaatta gagaccatcc agttgtccat tctctctcag ccacttacct
     1441 tcttccagga gggcggcctg ttgctgcgca tcaccggcaa agccataggc gccgttccag
     1501 aaatctacga tgtttccacc gggtggtatg gcaccggcgt agataccgtg acacattacg
     1561 gcagcatagc ctggcggcag gtcgggcggc acttgcgagg aaccgttgcc ggtgccgcca
     1621 ccgcctcctg gaccgcctga gaaagcgccc tgaccgccat tgctgcggat aaagggaagg
     1681 tctactgtat tgaaatcgac tgagccggtt gagaccacgg gcagcatggt gcgccccgtg
     1741 gagagtatgg cgttagctgc ctgcggtaag taattgtttg gtaaggtcgc ctgtgtggcg
     1801 ggggtgaatc cctgattcga atagttctgg ggccaggagc cgctagaagc gcctccgtta
     1861 aaggaccctc cgtcaaagga gctcagatcg aactgggcgt tttgagcgag tacaggtaaa
     1921 ggactgaccg cgccggtgag aaacagcggc gccattagta agggaagtag aatgcttctt
     1981 ttcacggacg ggagcctcat ttttaagtaa agagcctttt ccatgggcta aatctagatt
     2041 tatatcgtga cgctaacgtg acgacccgcc cgaactggca agggctgcca tcgctccatg
     2101 cttgaggctt catctggtca cagatcgggc aataagctat aaatgtagat tcgcgacaac
     2161 cgggagtcgg tacgtaaatg gcagtaagcc tcaaattaag tcaaaaggga gccattctgg
     2221 tcgccgtgcc ccttttcttt gagcttctct ttgttttcat tttggtaatg ctcttgcagc
     2281 aggctgatgc ggaagctcag cagcaggctc gctccagggc cattatttat caggcggatg
     2341 acctgtccag gatctttcat gaagctaatt tgacattact tggttacagc agcacccgca
     2401 gcccgctgtt ttacgatcgc ttcgaggagc tgaccggcaa aattcctgct gagattaagg
     2461 agctgaagga actcctgggc ggaagtgagg aaagtcggag cgaacttctg gcggataccc
     2521 agaagggcct tgacgctctc tgcaatgcca gaaagtttct tgttgaaaac agcggtaacc
     2581 tggctgattt tagcgccagg cacatgttta gtgcgatcgc ttccagtact aatgagctgg
     2641 agaccaagtt gaaggctttc atacacgagc aggaaagcaa gcagcatgaa cacgaagtgg
     2701 cacagatgtt ggccagaacc aggcttaagt ggctgattgc tgttggagtg cttttcaaca
     2761 ttttgatttc cctggccctt acggttttct tcgtcagagg cataggcagg cgcgtggacg
     2821 ttttaagaga taatgcctac agactggcgg ccgggcagaa actcaatgcc ccggccttcg
     2881 gcgatgatga gatatctgag ttggatggcg tctttcacca gatggcccgc tccctgaggg
     2941 aggcggccca gagggaaagt gcaatattcg aaaatgctat ggatgtgatt tgttctatcg
     3001 accagaacgg acgttttgct acggtcagtc cggcttcgct caaacttctg ggctttgatc
     3061 ccgaagattt gatcggt
//
LOCUS       DAOQNZ010000333         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16492_length_3071_cov_3.920756, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000333 DAOQNZ010000000
VERSION     DAOQNZ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16492_length_3071_cov_3.920756"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..377)
                     /locus_tag="PKW73_07200"
     CDS             complement(153..377)
                     /locus_tag="PKW73_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73107.1"
                     /translation="MALRIEKYIYYLRIGDALLMIVLEILSTLMEFLTECHHDSDGGS
                     SSTTHSGFCMPLLWTLFATYMGIVVYNFFT"
     gene            complement(452..2137)
                     /locus_tag="PKW73_07205"
     CDS             complement(452..2137)
                     /locus_tag="PKW73_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73108.1"
                     /translation="MAKAIFGKGNIRQRQQMVRQKARQIAVKSTQRAPIALLLRQSVY
                     ALLIPSLLSLLACGQTAARAEGDQSQTESPKSATEESTLNSDVVVEKPTPGTNLNIEK
                     EGDYSQKLYLEGEKALSERRYETAEAYFKASLKELYRSTNRLHLQELELSMARLDLAM
                     QRNDQAYRALTKLAHGIKVDQHGNVNKSKGALLLSELAEAELRLSRFDEADKHIRQAM
                     RLMQKGYQGLPALKERGIAEGRLARVLSHRGLNQDAKEHFQAAVDLLEQEPGYKQLDL
                     ADLLRQEALFYRDIGARKTAAQIFERACAIKASASNSQKTRSLTGEIDYVWEPGSPHS
                     HEIIDQDFPLRYIEVNNTRVAVTVIDLWELAGLLICVTNTDEHRHVFGLGEVKFYKVV
                     KDPSSGVARSYEQIPQVDHSRIDRIRRERNIWDLTQNRPWLANIQKTRNFRGLVPPNG
                     HDLFRGPNVFGVWGEWPGISHVVPTRVAILPSRENVFYVDEAEEEGSRESGLIRSEGI
                     RQMGLLPISMEPLESRTGELFYLYPRNEDVEVRVTVGNTTYSFPFRCRKRRIN"
     gene            2408..>3071
                     /locus_tag="PKW73_07210"
     CDS             2408..>3071
                     /locus_tag="PKW73_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73109.1"
                     /translation="MFSSLCALLTLLALIPVISIEASAAPAAPATTNSAATARPGSKV
                     VASRHKKRSKRRGNAAFGHKSVAKSGPGKPLVLQKPQLDSSLTTTNEIKPVISDPESD
                     RLLRLGALKHREGQLSEAEENFRQVLGRDPQNVDAFFNLGALAEGRGDLITALGHYRA
                     AHALSPRDVQIGEAVQSLEGQLKRNPSLSVAPPVAGAVALPVANPGFRAVDRTVAVQP
                     FTL"
BASE COUNT          646 a          773 c          837 g          815 t
ORIGIN      
        1 ccgcagggtt ttgcgagacc tgggagtgga gttgagtttc ccctcttatg tggaaggcta
       61 tacggactgt ttgaaaaaat atcgctctgc ttccagcgtg ctgcgttaat tagagtctgg
      121 tgcggctgct ttcttctgtg ccggcgacga ggctaagtga agaagttata gactacgatg
      181 cccatgtagg tggcgaataa cgtccagagg agcggcatgc agaaaccgga gtgcgtggtg
      241 gagctgctgc cgccatcact gtcatggtga cactcagtga gaaattccat aagggttgaa
      301 agaatctcaa ggacgatcat caaaagggcg tctccaattc tcaggtagta aatgtatttt
      361 tctatcctaa gcgccacccc ttggcaggtg cctttaatta agcccttctt tagatttgag
      421 gtcctgattg aggctcagac tcagaccggc atcaattaat gcgtcttttg cgacagcgaa
      481 acggaaaact gtatgtggta ttaccgacgg tgacacgcac ttccacatct tcattgcgag
      541 ggtagagata gaataactct cctgtgcgtg attccagcgg ttccatagaa atcggcagga
      601 gacccatttg tcttatgccc tcggagcgaa tcaaaccgga ttcccggctg ccttcttctt
      661 cagcttcatc gacgtaaaag acattctctc ggctgggcaa gatggctact ctcgttggca
      721 ccacatggga aatacccggc cattcacccc ataccccaaa gacattggga ccacgaaaga
      781 ggtcatggcc gttgggtggt accagtccgc ggaaatttct tgttttctgg atgttggcca
      841 accaggggcg attttgagtg agatcccaga tattgcgctc acggcgaatg cgatcgatgc
      901 ggctgtgatc tacctgggga atttgttcat aggagcgggc cacaccggaa cttggatctt
      961 ttacgacttt gtagaatttt acttctccca gaccaaagac atgccggtgc tcatcggtat
     1021 tggttacgca aatgagtaaa ccagccagtt cccagaggtc tatgactgta accgcgaccc
     1081 tcgtgttgtt tacttctata tagcgcaatg ggaaatcttg atcgattatt tcatgggaat
     1141 gcggggagcc cggttcccag acatagtcaa tttcaccggt gagagacctg gtcttctgtg
     1201 aattactggc actggccttt atggcgcagg cgcgctcgaa gatttgagca gcggtttttc
     1261 tagccccgat gtctcggtag aagagcgctt cctgcctcaa taagtcggct agatccaact
     1321 gtttatagcc gggttcttgc tctaataaat cgacggcggc ctgaaagtgc tctttggcat
     1381 cttgattgag accgcggtga ctgagaacgc gagccaggcg accttctgcg atgcctcgtt
     1441 ctttcagggc gggtagacct tgatagccct tctgcatcag tctcatggcc tgtcttatgt
     1501 gtttgtccgc ttcatcaaag cggctcagtc tcagttcggc ttcagccagt tccgagagta
     1561 gtaaggcccc tttgctcttg ttgacgttgc cgtgttggtc gacctttatg ccgtgggcaa
     1621 gcttggtgag ggcgcgatag gcctgatcgt ttctctgcat ggccaggtcg agtctggcca
     1681 tgctcaactc cagttcttgc aggtgcaggc gattggtgct gcgataaagt tccttgaggc
     1741 tggctttgaa gtaggcttcc gcggtttcgt agcgcctttc ggaaagggcc ttttctccct
     1801 ctaagtatag cttctgactg tagtctcctt ccttctcaat gttcaagtta gtgcccggcg
     1861 tgggtttttc cactactaca tcgctattga gggtgctttc ttccgttgcc gattttgggg
     1921 actctgtttg ggactgatcg ccctcagccc gggccgcggt ttgaccgcag gcaagcagag
     1981 aaagcaaaga aggtatcagc agggcgtaaa ccgactgccg caacaacaag gctatcggcg
     2041 cccgttgcgt gctctttacg gctatttgcc tcgctttttg cctgaccatt tgctgccttt
     2101 gccgaatatt gcctttgccg aatattgcct ttgccaaatg ttgccctgga tagaccatta
     2161 actagctctc tagttcggcg gttcctattt tatagtgtac gctcgtttat acttccgtaa
     2221 ccagttaccc attacttggc tgagcggcaa attagagagt atctgcaaac ggcttgagca
     2281 aggggcgaag cgggaataat gaatgcaggc acaaattttg agaagttacg tcagatactt
     2341 tgtcgactcg tcgattgatt gtcttgtttg tctgagaggg gttcttcttg tgagaagaag
     2401 cggcactttg tttagctccc tgtgtgcgct tctgacgctt ctggccctga ttccggttat
     2461 atctatagag gcttcggcag ctccggccgc tccggccact acaaattcgg ccgctacagc
     2521 tcgacccggg tcgaaagtgg ttgcctcgag gcataagaag agatcgaaaa gaaggggcaa
     2581 tgccgctttc ggtcataagt ccgtcgctaa gtccggcccg ggcaagcccc tggtgctgca
     2641 aaagccccaa cttgattctt ctctaacgac gaccaatgag atcaagcctg tcatttcaga
     2701 tcctgaaagc gatcgcttgc ttcgcctggg ggctttgaag caccgggaag ggcagctatc
     2761 ggaagcggaa gagaactttc gccaggtact gggacgggac ccgcagaatg tggatgcctt
     2821 tttcaatctt ggtgccctgg ctgaaggtcg cggtgatttg atcacagccc tggggcatta
     2881 ccgtgcagcc catgcgctct cgccccggga cgtacagata ggcgaggccg tgcagtctct
     2941 ggaaggtcag ttgaaaagaa atcccagcct cagtgtggcg cccccggttg ccggtgcagt
     3001 tgctttgccg gtggcaaacc ccggctttag agccgtggat agaactgtgg ccgttcagcc
     3061 cttcaccttg c
//
LOCUS       DAOQNZ010000334         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16494_length_3071_cov_3.524204, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000334 DAOQNZ010000000
VERSION     DAOQNZ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16494_length_3071_cov_3.524204"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PKW73_07215"
     CDS             complement(<1..961)
                     /locus_tag="PKW73_07215"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNA73110.1"
                     /translation="MLRGAVMAGYSQYTEPGSEPEQDSEQERQLSLAVDFLKQGLLVA
                     FPTETVYGLGADAASEEALARLYAVKGRPTSHPVIVHLSRMEELEQWSRGPLELAFRL
                     AEQFWPGPMTLILPRAANVSLSLTGGQDSVGLRIPSHPLALALLSRSGPLAAPSANKF
                     GKLSPTTAEAVRQGLGTEVAAVLDGGPCQVGIESTIISLLHEVPHILRPGMLLPEDVA
                     TACGLEVVLSYLPDHSSPVVKAPGTLPSHYSPDTPVVLLAQEDLQSTLEELTLAGLSI
                     SLLVFQQNAALADCGCVAESLVVGPFSEPYARLLYARLKSLDQP"
     gene            complement(974..2365)
                     /locus_tag="PKW73_07220"
     CDS             complement(974..2365)
                     /locus_tag="PKW73_07220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNA73111.1"
                     /translation="MSSTVEVDKSLKPIKPAPAAAKLKGFAGLIGAALSQMPYGQLTM
                     VMPDGGRHVFGSGGKSPQGHYVEALIVVRNAGLFRRLVFFGHIGFAEAFMDGDWISDD
                     LASVFAWFLLNLPHCRLLEGTDKKNLLVNSLGFLNQCGHLLRGNNKVNSRKNISYHYD
                     LSNDLFQIFLDETMTYSSAYFGTSLDDTEWKLSLKDAQEAKFEALCRKLNLHEGDHLL
                     EIGCGWGGFAVYAVSKYGCRYTGITISKEQLKFAQERVDKLPSIKDKIEFRLVDYRNM
                     ARPAGGFSKIVSIEMIEAVGHEYYDVYFQKIDQLLGEDGLAALQMITCPDSRYDLIRE
                     NTDFIQKHIFPGSLLPCLARLHKATATTALSLHELTDMGNDYARTLMVWQERFESHLS
                     EVFALGFDQVFVRKWRYYLSYCAAAFAMRNISVVQAVYTRPNNLTLSHRQFLSRFAST
                     KDDRQACDFGALE"
     gene            complement(2362..>3071)
                     /locus_tag="PKW73_07225"
     CDS             complement(2362..>3071)
                     /locus_tag="PKW73_07225"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNA73112.1"
                     /translation="ESKSRVFALNRPALVSFYEADHLLHSGTGSLLERVHSCLAEHGF
                     REPVRRIELLTYPRILGYVFNPVSFYLIYGHGQEQEVPLAVLVEVSNTFKEMKVYLAK
                     ENVASGCLGERPVFRSVVPKHFYVSPFGKLDDCFDFIVPFPDERLKICIDTLDGVSGD
                     KVLVSSLSGKSLLFSDRNLLVEFFRHPLMTFKVIAMIHFQALLLLLKRTPFYTKEEKP
                     HLQTDVINPRRVREKRV"
BASE COUNT          829 a          832 c          766 g          644 t
ORIGIN      
        1 caggttgatc aagactcttc aaacgagcat acagaagcct ggcatagggc tcggaaaaag
       61 gccccaccac aagactctca gccacgcagc cgcaatcggc aagggctgca ttttgctgaa
      121 agactaagag agaaatagac aaacccgcca gggtcaattc ttcaagagta gactgcaaat
      181 cctcctgcgc caaaagcacc actggagtgt ccggagaata atggctgggc agggtacctg
      241 gcgccttcac cacaggggaa gagtgatcgg gcaggtaaga aagcaccact tccagaccgc
      301 atgccgttgc cacatcttca ggcaagagca tgcccggtct caaaatgtga ggcacttcgt
      361 gcagtaaaga aatgatggtc gactcgatac caacctgaca tggtccacca tccaaaaccg
      421 ccgccacttc cgttcccagc ccctgcctga cggcttccgc cgtggtggga ctgagtttgc
      481 cgaacttatt ggccgaggga gcagccagcg gaccggagcg cgagagcaag gcaagagcca
      541 gagggtgaga gggaattcgc agcccgactg agtcttgtcc tccagtcaga gagagagaaa
      601 cattggcagc ccggggcaga atcaaagtca tgggtccagg ccagaattgc tcagccagtc
      661 ggaaagccag ttcaagcggt cctctcgacc actgctccag ttcctccatg cggctcaaat
      721 gaacgataac cgggtgactg gtgggtcggc ccttaacagc ataaagtcga gccagcgctt
      781 cttcgctggc cgcatcagcg ccgagaccat aaacagtctc agtgggaaag gccaccaaaa
      841 ggccctgctt gagaaagtca acggccaggg aaagctgcct ctcctgctca gaatcttgtt
      901 ctggttccga acccggctcc gtatattgac tgtaacctgc catcaccgct cccctcagca
      961 taagtttgac ttcctattct agcgcgccga aatcacaagc ctggcggtca tccttggtag
     1021 aagcaaacct gcttaagaac tgacggtgag aaagagttaa attatttggt cgggtataga
     1081 cggcctgaac cacgctgata ttacgcatgg caaaagcggc ggcacagtag gagagataat
     1141 atcgccactt gcgcacaaag acctggtcga agcccagagc aaaaacttca gagaggtgcg
     1201 actcaaaccg ctcttgccag accatcaaag tgcgggcata gtcattaccc atgtcggtca
     1261 gctcatgcaa agaaagtgct gtggtggcgg tggctttgtg aaggcgcgcc aggcagggaa
     1321 gcagggaacc ggggaaaata tgtttttgaa tgaagtctgt attttcacgg atcaaatcat
     1381 aacgactgtc cgggcaggtg atcatttgca aggccgccag cccatcttct cccagcagtt
     1441 gatctatctt ttgaaaatag acatcgtaat attcatggcc gaccgcttcg atcatttcta
     1501 tggaaacaat cttactgaaa ccacctgccg gtctggccat attgcgataa tcgacgagcc
     1561 tgaattcaat cttgtcctta atggaaggca gcttatccac tcgctcttgg gcaaacttga
     1621 gttgctcctt cgaaatagta atgcccgtat agcggcatcc gtacttgctc acggcataaa
     1681 cggcaaagcc tccccaaccg cagcctatct ccagtaaatg gtcgccctcg tgcaaattca
     1741 gtttgcgaca gagagcctcg aacttagctt cctgggcgtc cttgaggctg agcttccatt
     1801 cggtatcgtc aaggctcgta ccaaagtagg cacttgaata agtcatggtc tcatccagaa
     1861 agatctgaaa aaggtcatta ctgagatcat agtgatagga aatattcttg cggctgttta
     1921 ccttgttgtt acccctcagt aaatgcccac actgattcaa aaagccaaga gaattgacaa
     1981 gcaggttctt cttatcggtg ccttcaagca agcgacagtg cggaagattg agcaaaaacc
     2041 aggcaaagac ggaagccaga tcatcactga tccaatctcc atccatgaag gcttcagcaa
     2101 aaccgatatg accaaagaac accagtcggc gaaacaagcc ggcattgcgt actacaatta
     2161 gagcctcgac atagtgcccc tgtggactct tgccgccgct accaaagaca tgcctgccgc
     2221 cgtccggcat aaccatggtc agttgcccgt atggcatttg actgagagcc gcaccgatca
     2281 agccggcaaa tcccttcagc ttcgccgctg caggtgcagg cttgatgggc ttcaatgact
     2341 tatcaacttc aacagtgctg ctcataccct tttctccctg acacggcgag gattgatcac
     2401 atcggtctgc aaatgcggct tctcttcctt ggtataaaac ggcgttctct tgaggaggag
     2461 cagaagagcc tgaaaatgta tcatggcgat taccttaaaa gtcatgagtg gatggcgaaa
     2521 gaattccaca agcaagttcc ggtcgctgaa gagcaagctc ttaccgctca agctcgatac
     2581 cagcacttta tcgccactta ccccatcgag ggtatcgatg caaattttta gtcgctcatc
     2641 cgggaagggc actataaaat cgaaacaatc atcaagcttg ccgaagggtg aaacataaaa
     2701 atgtttaggc accaccgagc gaaaaacagg gcgctccccc agacaaccgg aggcgacgtt
     2761 ctccttggcc aggtagacct tcatttcctt gaaggtgttg ctgacttcca caagcaccgc
     2821 aaggggaacc tcttgctcct ggccatgacc atagatcaag taaaaggaaa ccggattaaa
     2881 gacatagccc aggatgcgcg gataagtgag tagctcgatg cgcctcacag gctctctaaa
     2941 cccgtgttcg gccaggcagc tatgcacccg ctccagcagg cttcccgtac cggagtgcaa
     3001 taaatggtca gcctcataaa agctgacaag cgctgggcgg ttcaaggcaa acaccctgga
     3061 tttgctctcc a
//
LOCUS       DAOQNZ010000335         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16508_length_3070_cov_2.935987, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000335 DAOQNZ010000000
VERSION     DAOQNZ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16508_length_3070_cov_2.935987"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKW73_07230"
     CDS             <1..189
                     /locus_tag="PKW73_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73113.1"
                     /translation="EGMLKLANFYERHDRKADAGELIEQLRTLEKHQDKYGYCWRIAR
                     NFTGGYKFERVDANNLGR"
     gene            552..2477
                     /locus_tag="PKW73_07235"
     CDS             552..2477
                     /locus_tag="PKW73_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73114.1"
                     /translation="MSDQKESDKSEHLPKAPEVVLRNPDAQNARNSGKADIVSAERLR
                     TNQSDGSLRRYSDIDLSDQEQSVEISYGEKSTSRTNKLTEAELLQSQRKAALEAQSET
                     IEEMREAVKTTPALEPILALREHAQSLPPGQDKDKYRDLALSQAREVSPDMAASIRRR
                     EENLTSAGAGDMLKGNVSETHLGSDLEQAFSQLGKLPLDKQISVLCAGFAAGFEQYRH
                     DENEKSWGRIIGSVEGIGSAFTSVATVVDFGGAVLWNDKKVAGEIADKFGKDLATATF
                     EGIRLYAAAEKCLFDTGASGDFTLPFRQITAAGAALNEQWAALPPREQERIKYRLITE
                     CAADAAMGAGGAQAIGKAKTFTGVLDAMAEHAIKHGPKAFDRSKKALAGSIQEFFTPD
                     YAYAGIGKVKLLQEQAGDLADAFAMERRAYVSKLDPTHRLRPIEAARENARLKGGRWD
                     EDAWKKLTPEQKQDALVKDGYEVLIDPEPRQPIDSTELADAFRGDKTPFSPLDERGRR
                     KSFINENGDLVPADREGVFKGVDVDPAEHIVASSYPGAKQHSPFTSVGTNGVVWKYGD
                     GAGLKVNIQGLRKAIADGQVSGVSIIEHQDLIAAIERSNLSAFDKTMALRFAKHDNEF
                     LIRGVVPKRFLEHIGEN"
     gene            2478..2768
                     /locus_tag="PKW73_07240"
     CDS             2478..2768
                     /locus_tag="PKW73_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73115.1"
                     /translation="MLKLYHHQTFIGTVSGVCANGFDMHGLIDFGPEAAPFKPIFEYF
                     QKRERPSSEEPPFDEDLLWNWSMTDEAGKRQEVGLPGVFEESDGIHVMWRWL"
BASE COUNT          807 a          687 c          810 g          766 t
ORIGIN      
        1 gagggaatgt tgaagctcgc taatttctac gagagacatg acagaaaagc cgacgcaggc
       61 gagcttatag agcagctgcg aacactggaa aagcatcaag ataaatatgg ttattgctgg
      121 cggattgcgc gaaatttcac cggtggctat aaatttgaac gtgtggatgc gaataattta
      181 ggcagataat aaatgttagg tgaaccgacg ggacctcccg tctgcatagt gctctgataa
      241 catatggttt cattgtcttg aaaaagcttt gaaacgatga cttgggaagg gttttgggaa
      301 atgacttttc cagccctccc cccttttgcc ttttcttttt tttggtaccc gacttctggc
      361 gctttcaggg gtgagcgcgg ctagcaccct cattatcagt ctgtcgctgc tttttagtat
      421 cagtcgccac agtcgaagta cagggcttgc ctcgctattc gacctttaag accaggcaaa
      481 tcctcgaatc tctagccctg tttgaccttt aagccgcttg ctacaatgga agaacgtcaa
      541 aagggcctgt catgtccgac cagaaagaga gcgacaaaag cgagcattta cctaaggccc
      601 cagaagtggt gctgcgaaat cctgatgccc agaacgctag aaattctggc aaggctgaca
      661 ttgtatcggc tgaaagactg cgcacgaacc agtcggacgg ctcactcagg cgctattctg
      721 atatcgatct gtccgaccaa gagcagagtg tcgaaatttc ctatggtgaa aaatccactt
      781 caagaacaaa caagctaacg gaagccgagc ttttacaaag tcaaagaaaa gccgctcttg
      841 aggctcagtc tgagactatc gaagagatgc gagaagcggt taaaacaact ccagctcttg
      901 aacctatttt agctttaaga gagcacgcgc agtcacttcc gcctggtcaa gataaagaca
      961 agtacagaga tttagcctta tcccaggcaa gagaagtgtc accggatatg gcggcatcta
     1021 tccgccggcg ggaagaaaat ttgacctctg ccggcgccgg cgacatgtta aaaggtaatg
     1081 tcagcgaaac ccatctggga agcgatttag agcaagcatt tagtcagttg gggaagctgc
     1141 ctttagataa acagatctcg gtcttatgtg ctggattcgc cgctggattt gaacagtatc
     1201 ggcatgatga gaatgaaaag tcctgggggc ggataattgg ctctgtagag ggcattggtt
     1261 ccgcttttac tagtgttgcc actgtggttg attttggcgg tgccgttctc tggaacgaca
     1321 aaaaagtcgc tggagagatt gccgacaaat ttggcaaaga tctagccaca gccacctttg
     1381 aaggcatcag gttgtatgcc gccgccgaaa agtgtctttt tgatactggt gcttctggag
     1441 actttacttt accatttcgg cagataacgg ctgccggggc agcgctcaat gaacagtggg
     1501 cagccttacc tccaagagaa caggagcgca ttaagtaccg ccttattacc gagtgcgcag
     1561 cagacgccgc catgggggcc ggtggcgctc aggcaatagg taaggcaaaa accttcaccg
     1621 gcgtcctcga tgccatggct gaacatgcca tcaagcatgg tccaaaagct tttgatcgtt
     1681 ctaagaaggc tctagcgggc tctatacagg agttttttac cccggactac gcatatgcag
     1741 gcattggtaa ggtaaagctc ttgcaggagc aagccggtga cctggctgat gcttttgcaa
     1801 tggagcgccg cgcttatgtc tctaagctcg accctacaca ccgcttaagg ccaatcgaag
     1861 ctgcaaggga aaatgccagg ctgaaaggcg gacgatggga tgaggatgct tggaagaagc
     1921 tcactccgga gcaaaaacaa gatgctctgg tgaaggatgg ttatgaggtc ctgatagatc
     1981 cagagcccag gcaaccaatc gactcgacag agcttgccga tgccttcaga ggagacaaaa
     2041 cgccattttc gccgcttgat gaacgcggca ggcgaaagtc ttttatcaat gaaaacggcg
     2101 accttgtgcc tgccgacaga gagggcgttt ttaagggagt cgatgtagac cccgccgagc
     2161 atattgttgc ttcaagctat ccgggagcta agcagcatag cccttttacc agtgtgggta
     2221 caaacggcgt tgtttggaaa tatggcgatg gcgctgggct taaagtgaac atccaggggc
     2281 ttcgcaaggc aatagccgac gggcaagttt cgggtgtttc tattattgag catcaggact
     2341 taattgccgc gattgaacgt agcaatttat cagcatttga taaaacgatg gctttgcgct
     2401 tcgctaagca tgataacgaa tttttgatcc gaggggtagt acccaagcga ttcttagaac
     2461 atattggaga aaactaaatg ttgaagcttt accatcatca aacctttata ggcaccgtaa
     2521 gtggtgtttg tgccaacggt tttgacatgc atggcttaat cgactttggt cctgaagcag
     2581 ccccgttcaa gcccatattt gaatacttcc agaagagaga acgaccttct tctgaggagc
     2641 cgccgtttga tgaagatttg ctttggaatt ggagtatgac cgatgaagca ggcaagaggc
     2701 aggaagttgg cctgcctggt gtttttgagg agtcggacgg cattcacgta atgtggcgct
     2761 ggctatgata aagagagccc cgagagttta gccgggtggc tgtctcgggg cttcagtgcg
     2821 atcaagaaaa tagattgatt attatcggat tgtcagtcag ataactttct gttggatttt
     2881 agcgctgcta agcgatgact gtcgtaacag cttttctgct cacccttagc caacttacca
     2941 gaattccttg ctaacgctta cggatatttc gccattcctt gttccgccag attgagttat
     3001 tttcgagtta atggcagctt ttaaggcatt ttccgcttct gaacttgaag tatagataaa
     3061 cgaaacctgg
//
LOCUS       DAOQNZ010000336         3065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16548_length_3065_cov_4.398007, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000336 DAOQNZ010000000
VERSION     DAOQNZ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16548_length_3065_cov_4.398007"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /gene="proS"
                     /locus_tag="PKW73_07245"
     CDS             complement(<1..1111)
                     /gene="proS"
                     /locus_tag="PKW73_07245"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNA73116.1"
                     /translation="MRWSKLFAPTLKEDPANAESLSHRLLLRGGYIRPLGSGIYSLLP
                     LAQRVRNKVIDLIRFHLDNIGAQEFVLPALHPQEIWEQSGRLSAMGEIMFRLKDRKGA
                     PYVLGTTHEEIFTGIAVNELNSYKQLPQMWYQFQTKFRDEPRPRGGLLRVREFTMKDS
                     YSFDLDQEGLDKSFELHRQAYCKIFAALHLPYLMVEASSGSMGGSQSVEFMLVSKSGE
                     DTLVTCAACGYGANSEKACAAISYGGSMPSRIDAEVEKFPTPEIKTIKQLEEFAGGAP
                     AQDQIKTLVYAVDGLIKVVLMRGDHELNLAKLTDLFGATAIRPCQDEEIVAALGAHPG
                     SLGAVGVTSENHHLVSEIVADESLRGRFAMVTGANQ"
     gene            complement(1310..>3065)
                     /locus_tag="PKW73_07250"
     CDS             complement(1310..>3065)
                     /locus_tag="PKW73_07250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNA73117.1"
                     /translation="SSGAVNDYSQEQLLKELEQEKVQVIVQAPSGFSASLEGFAAGAP
                     GQTVKPELKILYTGRNPESSYAGIRMLSVLNSSKEKFMDERIKVAGIRVEEAPVLDMK
                     VVSPPADGPFASPFLARVLSSILVFVAFIGAIYPAIDLLTGERERGTLEPLVITPVSR
                     RCLFAGKLVSVTCISYMFVIANLIGFYVSQFFQPPLLDILPFPLAAKLPLLCAVWAAL
                     LMLPLCLSLAVSMLALAGMARTVQQAQGYFTSLMFVAMLPMGLGLSGDVHLNLPLSLV
                     PFLGSLVTINDILEARYNPALMGLSIVVSLLFTVLLVLVAAPTMEREDLMFGVEEAPA
                     RRYEAGKFGRELFLLCSAVFLLMFYLSQSLVVMHHIWGLLLTQIMVVFAPAFMLVYFW
                     LKLPVKTVFGLEKPSGGFVSCLGAALTAPLTFVAALAVAHLQSQFLPSSKALEKLMEQ
                     ILGLGHEPLWLLLLIVALSPGICEELLFRGLIFSLLRKRLRQGQLILAVGALFGLFHM
                     SVMRFLPTAILGMLLTFIRLRSRSIYPCMCLHAAHNGMAVLLASHFKADLPAHYLVIA
                     GLTGLCGLSIFLWSTRKA"
BASE COUNT          826 a          824 c          819 g          596 t
ORIGIN      
        1 cctgattggc gcccgtaacc atggcaaagc gtccgcgcaa gctttcatcg gccacaattt
       61 cagacaccag atgatgattt tcgctggtga cgccgacagc gccgagggaa ccggggtgcg
      121 caccaagagc ggcaacaatt tcctcatcct ggcagggtct tatggcggtg gcaccgaaca
      181 gatctgtgag tttggccaga ttcagttcat ggtcgccgcg catcaagact actttgatca
      241 gaccgtcaac agcgtaaaca agtgtcttga tctggtcctg tgcaggagca ccaccggcaa
      301 attcttccag ctgtttgatg gtcttgatct cgggggttgg aaatttttcc acttccgcat
      361 caatccggga cggcatgctg ccgccgtaag aaatggcggc acaggctttc tcggagttag
      421 ctccgtagcc gcaagccgca caagtgacga gagtgtcttc cccacttttg gagaccaaca
      481 taaactccac cgactgactg ccgcccatgg agccggaact ggcttccacc atgagatagg
      541 gcagatgcag agcggcgaaa attttgcagt aggcctgcct gtgcaactcg aaagacttgt
      601 cgagcccctc ttgatctaga tcgaatgaat aggagtcttt catggtgaat tcgcgcacgc
      661 gcaaaagccc accgcgcggt cttggttcat cgcgaaactt ggtctgaaac tgataccaca
      721 tttgcggcag ttgcttgtag ctattcagct cgttgacggc aatcccagtg aagatttcct
      781 cgtgggtggt gcccagaaca tagggcgccc cctttctgtc tttcaaacga aacataatct
      841 cgcccatggc ggaaaggcgt ccgctctgct cccagatctc ttgcggatgc agcgccggta
      901 agacaaattc ctgggcaccg atattatcca agtggaagcg aatcagatct atcactttat
      961 tgcgtacacg ctgggccaga ggcaacaaac tgtagatgcc cgaacccagt gggcgaatgt
     1021 agccgccccg gagtaaaagc ctgtggctca gactctcggc attagccggg tcttccttca
     1081 gggtgggagc aaacaacttg gaccagcgca taattttttc cttgtaggta aataaaacaa
     1141 aagcccctta gcggggctca tgaccaatct aattgatgtc aggtgtcagc cccaaagtga
     1201 ttttggagtg actggtggtc ttctatctga ggcattcatc atggctctat tatggcaagg
     1261 ccttagcccc tgtcaaccaa gctgagtgtt tatttacaaa gggacggagc taggctttgc
     1321 gagtgctcca gagaaagata gagagcccgc aaagcccggt gagcccggca attacaagat
     1381 aatgagcggg cagatctgct ttaaaatggg aagcaagcag cacggccatt ccgttatggg
     1441 cagcgtgcaa gcacatgcag ggataaatgg agcggctgcg cagacgtata aaggtaagca
     1501 gcatgcccaa tatcgctgta ggcaagaaac gcattacact catatgaaag agaccaaaga
     1561 gggcaccaac agccagaatc aactggccct gccggaggcg ctttctcagc aaagagaaaa
     1621 tgagaccgcg aaagagcagt tcttcgcaaa tgccgggact gagggcaacg attagaagaa
     1681 gcaaccagag cggctcatga ccaagaccga gaatctgctc catcaatttc tcaagggcct
     1741 tgctgctggg aagaaactgc gactgcaagt gggcaacagc aagggccgcc acaaaggtaa
     1801 ggggcgccgt cagggccgct cccagacagg aaacgaaacc acctgaaggc ttttccagac
     1861 cgaatacggt cttaaccgga agtttgagcc agaaataaac aagcatgaag gcaggagcaa
     1921 agaccaccat aatctgggtg agcaaaagcc cccagatatg atgcataacc acaagggact
     1981 gggagaggta aaacatcaag agaaaaactg ctgagcaaag caagaaaagc tcacgtccaa
     2041 actttccggc ttcataacgg cgcgccggtg cctcctccac cccgaacatc aggtcttccc
     2101 tctccatggt gggcgccgcc accagcacca aaagcacggt aaagagcagc gaaacaacga
     2161 tggacaagcc cattagagcc ggattataac gggcctccag gatgtcgtta atagttacaa
     2221 gactgcccag gaaaggcacc agcgaaagtg gcagattcaa atgcacgtca ccgctgaggc
     2281 caagccccat gggtaacatg gccacaaaca tcaatgaggt gaaatagccc tgagcctgct
     2341 gcaccgtgcg cgccatgccg gccagagcca gcatggaaac agccagactc aagcagagtg
     2401 gcaacatcaa aagggccgcc cagaccgcgc aaagcagcgg caatttagca gccagcggaa
     2461 aaggcaggat gtccagcaag ggcggctgga agaactgcga aacatagaag ccaatcaggt
     2521 tggcgatgac gaacatataa ctgatgcagg taacgctcac tagctttccg gcaaagaggc
     2581 agcgacgact tacgggagtt atgaccagtg gctccagggt gccgcgctcc cgctcgcccg
     2641 tgagaagatc gatggccgga taaattgcac caataaaggc cacgaagacg aggatggagg
     2701 aaagtactct ggccaggaaa gggctggcaa acggtccatc ggctggaggc gagaccacct
     2761 tcatatccag caccggcgct tcttccaccc ttatgccggc caccttaata cgctcatcca
     2821 tgaatttttc tttgctgctg ttcaagaccg aaagcatacg aatgccggca taactactct
     2881 ccggattgcg tcctgtgtag agaatcttca gttcaggctt cacggtctgt ccgggcgctc
     2941 cggcggcaaa gccctcaaga cttgccgaaa aacccgatgg agcctgcact attacctgaa
     3001 ccttctcttg ctccagttct ttcagtaact gttcctggct gtaatcgttc acagcgccgg
     3061 atgag
//
LOCUS       DAOQNZ010000337         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16578_length_3062_cov_3.352843, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000337 DAOQNZ010000000
VERSION     DAOQNZ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16578_length_3062_cov_3.352843"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..1340
                     /locus_tag="PKW73_07255"
     CDS             396..1340
                     /locus_tag="PKW73_07255"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNA73118.1"
                     /translation="MSDKSGPINPNLIIPSGTQVVTLVEVRRRDGEPMRRAGSVGKIV
                     KAPTDASHAYLVLFPDGEQASFHRQEIAIRKHVQSLNFDRPQSVLGDRDLYEYVIYRC
                     VIGSRAYGLDVEGSDIDRRGIYLPPADLHWSLYGVPEQIEDVPAQECYWELQKCMMLA
                     LKANPNVLECLYSPLVELATPLAQELLDQRARFLSKLVYQTYNGYVLSQFKKLEQDLR
                     TTGELKWKHVMHLTRLLISGITILKEGQVPVRVVEHRDKFLAIRKGEVDWLEINAWRL
                     ELHKEFDQAYEHTKLPERPDYEWANEFLVSARGRMVSR"
     gene            complement(1371..2144)
                     /locus_tag="PKW73_07260"
     CDS             complement(1371..2144)
                     /locus_tag="PKW73_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73119.1"
                     /translation="MTEMDSLKRWRARIAVAHMAYDAGYYNQAIRHFRLALELAQTAE
                     LDETLKARSQSGLAKSLAALGKFEEAERLIRSALQIDESVSEAIVEQAEDYHQLALLC
                     WRSGRNSESMSFAQKAFDLAQKAGTAAPDDLKAKLLKHFAVLAEQSGDFMETERCLNR
                     AVEFIEGSNQLGKNSMIYGDVLLVKVLFLAEQGRFDEAAELYPYAMQIVALTRGVSHP
                     RVEEAMKIFQEMDSKDTHPNDEKLMKMASERDSSNHGIL"
     gene            complement(2354..>3062)
                     /locus_tag="PKW73_07265"
     CDS             complement(2354..>3062)
                     /locus_tag="PKW73_07265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA73120.1"
                     /translation="LGKSALETGLGQTALAEIKEGAAVVAAKAGVKLSQEAMEIGAME
                     IGAGTASKGTIQTMSDLFRRHPGEVVTAVPRRYLGGGGDGIKAHTLSIDILHPDELFT
                     RVATQRQNANLFGKQLENGIETRRLHYLNVNEAREQAHVTLSLGNRIAGIGGVRSNRM
                     DPTELWVDHISVEAKHQGKGYARHIIESIYDYAQRRNQQVVPSGFTTMGQRLKPIFME
                     MNERYPSAASPLPFRNF"
BASE COUNT          631 a          780 c          819 g          832 t
ORIGIN      
        1 gtgacaagtg aaacgattgc ggtgctgggt tcttgggatt gtgccttcgg aaaaaggtgc
       61 tagagtggct ctggctgcgc ttcgcggcgc ctctcggcgg ggcgttcgtt gccgatttca
      121 ggggtatccc tgaggcacat tcattatttt tactgacatg ctttcagggt aaatcccagt
      181 cgtatctttt tcaccaaacc ttgcctttcg aaatatccga attgcagttg ttccttcttg
      241 attctcagtc ttggtagggg caacatgcaa ttatgctatt ctcgtgcttg gctacagttg
      301 tgaatatccc tcccgctgcg tgttggaaag tctttctgta tccacaaagc ccccggctat
      361 atggcaatat ctcgcatcca accgaaggca gacttgtgag cgacaaatct ggacccatca
      421 atcccaacct tattattcca tcgggcaccc aggtggttac ccttgtggaa gtgcggcggc
      481 gggatggaga gccgatgcgg cgggccggca gtgtcggcaa aattgtcaaa gcgccgaccg
      541 atgcctcgca tgcctattta gtgcttttcc ccgacggcga acaagctagt tttcaccgtc
      601 aggaaattgc tattcgcaag catgtgcaga gtttgaactt tgaccgcccc cagtcggtcc
      661 ttggtgatcg agacctctat gaatatgtga tttaccgttg cgtcataggt tcgcgggctt
      721 acggactgga tgtggagggt tccgatatag ataggcgcgg catctacctt ccgcccgccg
      781 atttgcactg gtcgctatac ggcgtgcccg agcaaataga agatgtgccc gcccaggagt
      841 gctactggga attacagaaa tgtatgatgc tggctttgaa agccaatccc aatgttctag
      901 agtgtctcta ctcgccgctg gtggaattgg cgactcccct ggcgcaggaa ctactcgacc
      961 agcgtgcccg ttttttgtcg aagcttgtgt accagaccta caacggttat gtgctttctc
     1021 agttcaaaaa gcttgagcaa gatttgcgca ccaccggtga gttaaagtgg aagcatgtga
     1081 tgcaccttac gcgcttgctc atatccggta tcacgattct gaaggaaggt caggtaccgg
     1141 tgcgggttgt cgagcaccgt gataagtttt tggcgattcg aaagggtgag gtagactggt
     1201 tagagatcaa tgcctggcgg cttgagctgc acaaagagtt tgatcaagcc tacgagcata
     1261 ctaagctgcc tgagcgcccc gactacgaat gggcaaacga gtttcttgtc tctgctagag
     1321 ggcggatggt tagtcgttag aggcgccagt ggctgagctg aaggtgcgag ctaaagaatc
     1381 ccgtggttgc tggaatctct ttcgctggcc atcttcatga gtttttcatc gttcggatgg
     1441 gtatcttttg aatccatttc ctggaagatt ttcatggctt cttccaccct cgggtgtgag
     1501 acgcctctag tgagggccac aatctgcatg gcatagggat aaagctccgc cgcctcatca
     1561 aagcgcccct gctcggccag aaagagcact ttcaccagaa gtacgtcacc gtagatcatg
     1621 ctgtttttgc cgagctggtt gcttccttct atgaactcta cagcccgatt caggcagcgt
     1681 tctgtttcca taaaatcgcc cgattgttca gcaagaactg cgaagtgttt caatagcttt
     1741 gctttcagat cgtcaggggc agcggttcct gccttctgag ccagatcaaa ggctttctgg
     1801 gcaaacgaca ttgattcgct gtttcgccca cttctccagc aaagaagtgc aagttggtga
     1861 tagtcttcgg cctgctccac aattgcttcg ctgacgcttt catcgatttg taatgccgag
     1921 cgtatcaggc gctccgcttc ttcgaatttt cccagagcgg ccagtgattt agccagaccg
     1981 gattggcttc tagcttttag tgtttcgtcc agttcggctg tttgagccag ttccagtgcc
     2041 agtctaaagt gtctgatcgc ctggttgtaa taaccggcat catatgccat atgcgcgacg
     2101 gcaatgcgtg cccgccaacg tttcagagag tccatctcag tcatgattta tgccctcttt
     2161 tgcaaagcaa gccccggctg tctatttcta ccgccaggta agttgtcacc ggcagtttga
     2221 gcttctctgc gagcctgcaa gctatagatt gtatgcaata ttgagcggtt tgcctatagc
     2281 cggaacgatt gcatagtctc ttggcaggtg caaaagcctg tcggcgtaat ctcgctaaac
     2341 ttgaacagtt gtcctagaaa ttacggaagg gcaaaggtga tgcggcactg gggtagcgtt
     2401 cgttcatttc cataaagata ggcttcagtc tctgacccat ggtggtgaac ccgcttggca
     2461 ctacttgctg attccggcgc tgggcgtagt cataaattga ttcaatgata tgcctggcat
     2521 agcctttgcc ctgatgttta gcttccaccg aaatgtggtc gacccagagt tcggtcggat
     2581 ccatgcggtt ggagcgcacc cctccgattc cggcaatgcg attgcccagg gacagagtta
     2641 catgggcctg ttcgcgcgcc tcatttacgt tgaggtagtg cagacggcgg gtttcgatgc
     2701 cgttttccaa ttgctttcca aacaaattgg cgttttgcct ctgggtggcg acgcgggtga
     2761 aaagctcatc gggatgcaaa atgtcgattg atagagtgtg ggctttgatg ccgtctcctc
     2821 cgccgccaag gtaccggcgc ggcaccgccg ttaccacttc acctggatgg cgccggaaga
     2881 gatcggacat ggtttggatt gtgcctttcg aggccgtacc ggcaccaatt tccatggcac
     2941 caatttccat tgcttcttgc gataatttca cgccggcttt ggctgctacg actgctgcgc
     3001 cttccttgat ttcagccagt gccgtttgac ccagccctgt ttccagtgct gatttgccta
     3061 gg
//
LOCUS       DAOQNZ010000338         3060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16599_length_3060_cov_2.805324, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000338 DAOQNZ010000000
VERSION     DAOQNZ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16599_length_3060_cov_2.805324"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKW73_07270"
     CDS             <1..485
                     /locus_tag="PKW73_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73121.1"
                     /translation="SEGINPNPKVESPGLGDINSSVQAHSRWREPVAILCDYPDSAYP
                     LLFNLDRTPGTYLINGRPVRHLMRLEQLSMLTGHWKELKDTILRNLEAEFSSSRAELV
                     LVSGCHLQDYVQQNGLLDLLRENYDQGAPCIFLSDNRQPREFIGFYYGYDTWLRRKSK
                     "
     gene            515..2380
                     /locus_tag="PKW73_07275"
     CDS             515..2380
                     /locus_tag="PKW73_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73122.1"
                     /translation="MVALGLLLLSGANFLYWSMHPLSISADQSVYVQSGLLLLQGKVP
                     YLDFFDFNPPLIVYLNVIPVLVARALALPLPMGLNMTVMTLNVISTLFSAFILYRYAA
                     QCRPAVRLVEMLPVLAMYAAFTQALVTDFGQREHLFTIAFMPFFVLRGLRHAGASPSR
                     LISCLSGLLAGLLMSLKPQFFLIWFFSELGFLCQGVQSFRFPSTVKPAIAPELLALLL
                     PAFVYALSFFFLPRQALKVFFDVALPIYLFGNNWGARSFMPMLVGGDLFAWPFYQFLA
                     GALIYTVLRRESIWLSPLLMICLASLFNYLSGGQAWTYRLLPFSLYSKLLLALEVSVL
                     LRFLYDRFKEGFRQTGALASAVFLLCSGYLSSRFIAESVEELVGAEGEAFDLKVIGYS
                     GTNIRSELDPTFFCMVENSHLGDKVLYIGSGIRPGYPAQLQCGRRPGSRYLYGYPLTM
                     LDQARENRPDLTARFKALEARVVAELGEDISKNKPLLVFVQRCPTGLLLAPYHFISRY
                     LQNYSLIGEVDGAGVYKLTGSKVDLKKYSVGEREAMVLQVICGQKTTSEVATEKGLPV
                     RLVEDWVERARSGMRSNLDDRTLDERGLLKLKIEELNSEIWQLNRQVDKLRNGGR"
     gene            2462..>3060
                     /locus_tag="PKW73_07280"
     CDS             2462..>3060
                     /locus_tag="PKW73_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73123.1"
                     /translation="MNKTPALLLALASALISAPVFADAVSWERLSKEALAAREGKELD
                     KAVGLYRQAVEDARSSGLEPKFIALSLNDLGLCLLAAGQKGEAEKYLLEAIAVREKAY
                     GAQEPTLVPTLGNLGQYYKGEKQWDKARQSYERAIAIIEKNSSDKDVKLIPLVNALAA
                     LSMEAGANQQALDQLGRVKELAAKSGDKGAQIIALNNMAV"
BASE COUNT          631 a          696 c          844 g          889 t
ORIGIN      
        1 atagcgaggg aattaaccca aatcctaagg tggagtctcc cggtcttggt gatatcaatt
       61 catccgtgca agcccattcc cgttggcggg agccagtagc cattctttgt gactatccag
      121 attctgctta tccgcttctc ttcaatcttg atcgtacccc aggcacttac ttgataaatg
      181 gtagacctgt gcggcatttg atgcgccttg aacagctcag catgttgact ggccactgga
      241 aggagctgaa ggataccatt ttgcgtaatt tggaggctga gttctccagt tcaagggccg
      301 agctggtgct ggtgagcggc tgtcatttgc aagattatgt acagcagaac ggattgcttg
      361 atcttttgcg tgaaaattac gatcagggcg ctccctgtat tttccttagt gataataggc
      421 agccgagaga gttcataggt ttctattatg ggtatgatac ctggctccgg aggaagtcta
      481 agtgatttct cttaggaatc agataccagt ctttatggtt gcattgggct tgcttttgtt
      541 gagtggagcc aacttcttat actggtccat gcatccgcta tccatcagcg ccgaccaatc
      601 tgtgtatgtt cagagtggtc ttttgctctt gcagggaaaa gtgccatatt tggatttctt
      661 tgattttaac ccacccctga ttgtatattt gaatgtgata ccggtgctgg tggcgagggc
      721 tttagccctg cccctgccca tggggttgaa catgactgtc atgaccctta acgtcatcag
      781 cacactcttc tccgctttca tattatacag gtatgccgct cagtgccgac cggcggtgag
      841 gttggtggaa atgctgcctg tgttggctat gtatgctgcc tttacgcagg ctctagtgac
      901 cgattttgga caaagggagc atctatttac tatcgccttc atgcctttct ttgtgctgcg
      961 tggattgcgc catgctggag ccagtccgag tcggctcatc agttgcctgt ccgggcttct
     1021 agctggcttg ttgatgtctc tgaagccgca atttttcttg atttggttct tttctgagtt
     1081 gggtttcctc tgccagggtg ttcagagttt tcgttttcct tcgaccgtta aaccagcaat
     1141 tgctcccgaa ttgttggccc ttcttttgcc agcatttgtt tatgctttga gctttttttt
     1201 cttgccaaga caggcgctga aagtcttctt cgatgtggct ttgcctatat atttattcgg
     1261 caacaactgg ggagcgcgaa gctttatgcc catgctggta gggggagatc ttttcgcctg
     1321 gcccttctat cagttcttgg ccggggcttt gatttacacg gtcttgcgtc gggaaagcat
     1381 ctggctttcg ccgctactaa tgatttgtct ggctagttta tttaactact tgagtggtgg
     1441 ccaggcttgg acctacaggc ttctgccctt ctcgctctat agtaagctct tgctggctct
     1501 ggaagtaagt gtcctcttgc gttttctgta tgatcggttc aaagagggct tccgccagac
     1561 tggcgctctc gcatcagctg tttttttgct ttgctccggc tatttgtctt cccgttttat
     1621 tgcggagtcc gttgaagaat tggtgggtgc ggagggggag gcttttgatc tcaaggtaat
     1681 tggttattcc ggcaccaata ttcgtagtga attggatcct acctttttct gcatggtgga
     1741 gaacagtcat ttaggcgaca aggtactgta tattggttcc ggcataaggc cgggctatcc
     1801 tgcgcaactg caatgcggtc gtcgccccgg cagccgttat ttgtacgggt atcctctcac
     1861 catgcttgac caggcaagag agaaccgacc cgatttaaca gcacgcttta aggctcttga
     1921 ggcacgtgtc gttgccgaac tgggtgaaga tatcagcaag aataaaccct tgctggtttt
     1981 cgtacaacgt tgccccaccg gccttttgtt ggcgccttat cattttatct ctcgctattt
     2041 gcagaactat tctttgattg gagaggtgga tggagcaggc gtctataagc ttaccggtag
     2101 caaggtcgac ttaaagaagt attcggtggg cgagagagaa gcgatggtat tgcaggtcat
     2161 ctgcgggcaa aagacgacta gtgaagtcgc taccgagaag ggtttaccgg ttcgcctcgt
     2221 ggaagactgg gtggagcgcg ccagaagcgg catgcgcagc aacctggacg atcgcactct
     2281 tgatgaaaga ggtttgctca agctcaagat tgaagagctg aatagtgaaa tctggcagtt
     2341 gaatcggcag gtggataaac tccggaacgg cgggcgctga ttgctagtta aatcgtgttt
     2401 gaggtgtttg tcttgcgatt ttgctatagt ctgagttgtt caccggtaga gataaaaagt
     2461 cttgaataaa acccctgcgc ttctgttggc cctggccagt gctctgattt ccgccccggt
     2521 cttcgccgat gccgtcagtt gggagaggct gtccaaggaa gctctagctg cccgggaggg
     2581 caaagagctt gataaagccg tgggtttgta ccggcaggca gtagaagatg cccgcagcag
     2641 tggactggag cctaagttta ttgctctttc cttgaacgac ctggggctct gtttgcttgc
     2701 cgccgggcag aagggtgaag ctgagaaata tctacttgag gccattgctg ttcgggagaa
     2761 ggcttacggt gcccaggagc ctactctggt gccgactctg ggcaatctcg gtcagtatta
     2821 caagggcgag aagcaatggg acaaggctcg tcagtcatat gagcgggcca ttgctatcat
     2881 tgagaagaat agttcagata aggacgttaa gcttattcct ctggttaacg ccctggccgc
     2941 tctttcaatg gaagccggag ccaaccagca agccctcgat cagttagggc gggtgaagga
     3001 gttggcggcg aaaagcggcg acaaaggcgc gcagataatc gccctgaata atatggctgt
//
LOCUS       DAOQNZ010000339         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16616_length_3058_cov_3.928072, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000339 DAOQNZ010000000
VERSION     DAOQNZ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16616_length_3058_cov_3.928072"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PKW73_07285"
     CDS             complement(<1..1030)
                     /locus_tag="PKW73_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA73124.1"
                     /translation="MFSSRQSQQGKGNAPIPRGFRLPKVSVLPSYTDIQFLHQEAVKV
                     PGRLVEQPFSVTEGSQEYLLAVKFEQEIGLPVWTLTQVSSQGHQVIFQHAESDVSLVA
                     NIVMSGCGCDPLGDSLAADTFMGTDSMSNLKPIGNTSAEVPTITGTESAILFDAPRPG
                     MKATLEGDLTKMQAPNLLQSITMSKMTGRLDVKDKGETAKVYFVDGTPMHAELKEVGG
                     DDAVIELITWTTGEFRFWPDETSPHQTVKRRLDAMLMEGVTLLDQSKYLESANLKMES
                     ILVKRMNIISEQEFASKLQKGAPLPIKPQMELFEQVDGRSTFYDILRKKPMKKADWVP
                     ILFNLISCG"
     gene            complement(1257..2540)
                     /locus_tag="PKW73_07290"
     CDS             complement(1257..2540)
                     /locus_tag="PKW73_07290"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNA73125.1"
                     /translation="MVAVTSADKPTMECETEHFLGAQRQLDEIAEEMGLDRELHERLR
                     YPKRALIVTVPVRMDDGSVKSFTGYRVHHDVTLGPAKGGIRFHPEVNLGEVSCLAMLM
                     TWKCALMGLPYGGAKGGIRVEPWNLTPGENERLTRRYTSEIINLLGPDKDIPAPDMYT
                     NEQTMAWIMDTYSINVGHTVPSVVTGKPVSIGGSFGRTEATGRGVAYCVRRAVNHYGI
                     KGDAPSVVVQGFGNVGSVTAKLLHQAGFKVVGVSDVYGAIYNPKGLDIPRLIAYVSEM
                     GKVQGFNGSQDLDNKALLELPCDVLVPAALGGQITNLNADKLQCKIIVEGANGPTTPE
                     ADVVLHDKGVIVVPDILANAGGVTVSYFEWVQGMMHLFWTEDEVNQRLEQIMGRACDQ
                     VLELSTKSKLRPRMAALRIGVSRIAEAKRLRGLYP"
BASE COUNT          639 a          791 c          862 g          766 t
ORIGIN      
        1 gaccgcaact gatcaagttg aacaagatcg gcacccagtc ggcttttttc atgggcttct
       61 tgcgcaagat atcgtagaaa gtgctacgcc cgtctacctg ctcgaataat tccatctgcg
      121 gcttgatcgg cagcggcgcg cctttctgca atttgctggc aaattcctgc tcggagatga
      181 tgttcattct cttcacgaga atggactcca tcttcaggtt ggccgattcc agatacttgc
      241 tctggtcgag caaggtgacc ccttccatga gcatggcgtc cagacggcgt ttgaccgtct
      301 ggtgcgggct ggtttcatcg ggccagaagc gaaactcgcc ggtggtccag gtaatcagtt
      361 cgatcacggc atcgtcgccg cccacttctt tcagctcggc gtgcatgggc gtgccgtcta
      421 cgaagtagac tttggctgtc tctcccttgt ctttcacatc gaggcgcccg gtcatcttgg
      481 acatggtgat ggattgcagt aggttgggcg cctgcatctt ggttaaatcg ccttccagcg
      541 tagctttcat acccggtctt ggcgcgtcga agagaatggc gctttcggtg ccggtgatgg
      601 tcggcacttc cgccgaggtg ttgccaatgg gcttgaggtt ggacatggag tcggtgccca
      661 tgaaggtatc ggcagccaga ctgtcgccaa gcggatcgca accgcagccg ctcatgacga
      721 tgttggctac gaggctgaca tcgctttcgg cgtgttgaaa aatgacttga tgcccctgac
      781 tggatacctg ggtcagggtc cagacaggca gcccgatttc ctgctcgaat ttgactgcaa
      841 gcagatactc ctgtgaacct tccgtcaccg agaagggttg ttccaccagc ctgccgggta
      901 ccttgactgc ttcctggtgc agaaattgga tgtcggtgta ggatggtaaa acggaaactt
      961 tgggtaggcg gaaaccgcgc ggaattggcg cgttgccttt gccctgttgt gattgcctag
     1021 atgaaaacat gccgatacca gaaaagccat cagtacatca gtacagaaac tgtttcacgc
     1081 ctgacctgaa agtctgacca aaaagcctga cactaattat tatcagtttg ccacatatat
     1141 gagtctatga aacaaaaata gaggtagaaa agagacaccc ccccgcaaga aacgaggggt
     1201 gtctttctct tggcattgcc ggcagcttgt gccggcgatt gctacttact tcgggcttat
     1261 gggtagagac cacgcaggcg cttggcttcg gcgattctgg aaacgccgat gcggagagcg
     1321 gccatacggg ggcgcagttt gctcttggtg gagagctcaa gcacctggtc gcaggcgcgt
     1381 cccataattt gttcgaggcg ctgattgact tcatcttcgg tccagaagag atgcatcatg
     1441 ccctgtaccc actcgaagta gctgacggtg acgccgccgg cattggccag gatgtcgggt
     1501 acgacaatca cgcctttgtc gtgcagaact acgtcggctt ccggggtagt ggggccgttg
     1561 gcgccttcca caatgatttt gcattgcagt ttgtcggcgt tcaagttggt gatttgacca
     1621 ccgagagcgg caggcaccag cacgtcacag ggcaactcta agagcgcctt gttgtcgaga
     1681 tcttgggagc cgttgaagcc ctgtactttg cccatttcgg atacgtaggc gatcaagcgc
     1741 gggatgtcga gccctttggg attgtagatg gcgccgtaaa catcggaaac gccgactact
     1801 ttgaaaccgg cttggtgcag gagtttagcg gtgacggagc cgacgttgcc gaagccctgt
     1861 actaccacgg aaggcgcatc acctttgata ccgtagtggt tgacggcgcg gcgcacgcag
     1921 taggcaacac cgcgaccagt tgcttcggta cgtccgaagg aaccgccgat ggaaaccggc
     1981 ttgccggtga ccacggaagg cacggtatga ccgacgttga tactgtaggt atccatgatc
     2041 caggccatgg tctgctcgtt ggtgtacatg tccggggccg gaatgtcttt gtcgggtccg
     2101 agcaggttga tgatttcgga agtgtagcgg cgcgtcaggc gctcgttttc accgggtgtc
     2161 aggttccaag gctctacgcg gatgccgcct ttggcgccgc cgtaaggcag acccataagg
     2221 gcgcatttcc aggtcatgag catggccagg caggagactt cacccaggtt cacctcaggg
     2281 tggaagcgga tgccgccttt ggcgggtccg agggtgacgt cgtggtgcac tctgtaaccg
     2341 gtgaaggact tgacggagcc gtcatccatg cgcaccggca cggtgacgat gagagcccgt
     2401 ttgggataac gcaagcgttc gtgcagctct ctgtctagac ccatctcctc ggcgatttcg
     2461 tcgagctggc gttgagcacc caaaaagtgc tcggtttcac attccatagt cggtttatca
     2521 gccgacgtga ccgctaccat gcagagttcc tccagttaga gccaaaattc taaaggttaa
     2581 acgcctgaaa tattgaaagg tccatctata cattcagatt gtccgaagtt gcttccggac
     2641 ctacgacctt tcaaagacgt ttcaatatgt taacgcttat atgcaggcga tatggccata
     2701 cttaccgaaa ggcaagcact taattttttc tcagtccttg tccttaccgg ctttagaccg
     2761 cttggcctgc ctgtcccggt cgaaaattgg ccgccgggag gactaattaa atgtttatcg
     2821 ggtaagagta gagttatcct ctcttataga tgtggatggc cgataattaa gtcggctgat
     2881 ttttccttga tggtatcgtc tgtggtgcga tatcggccgg ctcctgattt atttcaacct
     2941 gattcatttc aattagcttc gctcagatcc ttaccattcc tttgaattat cgatgaaact
     3001 gtctttcgag cacattctca acgtcttttt agtcttcatt ccagtgccta ttcttggc
//
LOCUS       DAOQNZ010000340         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16636_length_3056_cov_4.257914, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000340 DAOQNZ010000000
VERSION     DAOQNZ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16636_length_3056_cov_4.257914"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKW73_07295"
     CDS             <1..344
                     /locus_tag="PKW73_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HNA73126.1"
                     /translation="CKPPRFKEGASVTVVAVNEEYPRSGSKDVPIEIGPMPEGVALFH
                     CGTAVKDGQVVTNGGRILAPTAVAATLDEARQLALQALAPERISFKGLAFRRDIACVA
                     KAVGEKICQST"
     gene            329..985
                     /gene="purN"
                     /locus_tag="PKW73_07300"
     CDS             329..985
                     /gene="purN"
                     /locus_tag="PKW73_07300"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNA73127.1"
                     /translation="MSIDIVLLASGRGSNVEAIADSILAGRLDARISCVISNNADAGV
                     LDVARRKGLTAHVLPSQGLKKAEHEEALLALLAEHNYAPDFVVLCGYMRILTGRFLRA
                     FLSPAGFFRVINIHPSLLPSFPGATAYADAYKAGVAVSGITVHLVDEQVDHGPILAQV
                     SFPRHESDSFEDFSLRGLALEHKLLPEVLARIERQGGIDLSRLPFDMEELLAQCGGKQ
                     "
     gene            982..>3056
                     /locus_tag="PKW73_07305"
     CDS             982..>3056
                     /locus_tag="PKW73_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNA73128.1"
                     /translation="MNLKARELESKDDKAGQDPTLKQFAALVLPSHGGSSTASRLACP
                     LYWFALEKPSSEVEARLEKMSGELLCDSVDEVFCFAPLDKVSDFAGHIDGGFKFAAVR
                     QLKSGVTDNTANSVAQALAIMGLGVEVQVSAGTLFLSVDSLENVAHNSLTEKVKHFQL
                     GGQMALPPIPERRHSVCGGYEVVDINLADQELVDLSKERLLSLNLEEMQTVKGYFADQ
                     AFQNERKHHGLPSSPTDVELEIIAQTWSEHCKHKIFNATIEHTDENGRTEIIKSLYKS
                     YIKAATEKLKSERPDLLSVFEDNAGVVAFDEEYAVCFKVETHNSPSALEPYGGALTGI
                     LGVNRDILGTGLGAKPIFNTDVFCLPHPDSPFVAGLKHDKLLSAKEIVCGVRKGVQDG
                     GNKSGIPTVNGAVFFDDSYRAKPLVYCGTGGLLPLEAAGRDAVKKHTAKGDYIVMAGG
                     RVGRDGVHGATFSSLALDDNTGSDVVQIGDPFTQKRLLDFVLQARDGGLVTGLTDNGA
                     GGLSSSVGEMASITGGALLEIDSVPLKYPGLFDWQIVVSESQERMTFSTDKPDELLAL
                     AAKLDVEATVVGRFTDDGYFRVVRGGATVALLNLDFLHGGAPGLKLKSRWQPPAINAA
                     KPEVAATGRQLLSLLANPNICSREPVIRQYDHEVQGGSVIKPLMGPRQISPCDAGVLL
                     PRPSEKKGIA"
BASE COUNT          636 a          848 c          868 g          704 t
ORIGIN      
        1 agtgcaagcc gcctcgcttc aaagaggggg ccagcgttac cgtggtggcc gtcaatgaag
       61 aatacccgcg cagcggttct aaggatgtac ctatcgaaat cggcccaatg cctgaaggcg
      121 tcgccctctt tcactgcggt acggccgtca aggacggtca ggtggtgacc aacggcggcc
      181 gtattttggc accaactgca gtagcagcca cgttggacga ggccagacag cttgccctgc
      241 aagccctggc cccagaaaga attagtttca agggactggc cttcaggcgc gatatcgcct
      301 gcgtggcaaa agcggtgggg gaaaaaattt gtcaatcgac atagtgcttc tagcctccgg
      361 gcgcggctcc aatgtggagg ccattgccga cagcatactg gccgggcggt tggatgcgcg
      421 cattagttgt gtgatcagca ataacgctga tgccggtgtg ctggatgtag cccggcgtaa
      481 aggtctgact gcccatgtgc ttccttccca gggcttgaaa aaggctgaac atgaagaagc
      541 gcttctggcg cttcttgcgg agcataatta tgcccccgac ttcgtcgtcc tgtgtggtta
      601 tatgcgcata ttgaccggac gcttcctgcg ggccttcctt tctcccgccg ggttctttcg
      661 cgtcatcaat attcatcctt ccctgctacc tagttttccc ggcgccaccg cttatgccga
      721 tgcttacaag gccggcgtcg ccgtatcggg tatcacagtg catctggttg atgagcaagt
      781 tgatcacgga ccgatcttgg cgcaagtgtc tttccctcgc catgaaagcg acagcttcga
      841 ggacttttcc ttgcgcggct tagctctcga gcataagctt ttgccggaag tgctggcacg
      901 cattgagcgg cagggaggca tagacctctc ccgcttgcct tttgatatgg aagaactgct
      961 cgcccagtgc ggaggaaagc aatgaatttg aaagccaggg aattagagag caaagacgac
     1021 aaagccgggc aagacccaac tctcaagcaa tttgcggccc tggtgttacc ctcgcatggt
     1081 ggttcttcta cggcttccag gcttgcctgt cctctctact ggttcgccct ggaaaagccc
     1141 agtagcgaag ttgaagccag actggagaaa atgtccggag agttgctctg cgattcggta
     1201 gacgaagtct tttgtttcgc accactggat aaggtatctg actttgccgg tcatattgat
     1261 ggcggcttta agttcgccgc tgtgcgccaa ctcaagagcg gtgttaccga caatactgcc
     1321 aattcagtcg cacaagccct ggccattatg gggctgggag tggaagtgca ggtttccgcc
     1381 ggcactttgt tcttgagtgt ggattccctg gaaaatgtcg cccacaacag cctcaccgag
     1441 aaggtgaaac actttcaatt gggcggccaa atggccctgc ctcctattcc ggagcggcgt
     1501 catagtgttt gcggcggcta tgaagtggtg gacatcaatc ttgccgatca ggaactggtt
     1561 gatctcagta aggaacggtt gctttccctc aatctcgagg agatgcagac cgtcaagggc
     1621 tactttgccg accaggcttt tcaaaacgaa cgcaagcacc atggcttgcc gtcctcgcca
     1681 accgatgtgg aactggagat aattgcccag acctggtctg aacattgcaa gcataaaatc
     1741 ttcaatgcca ccattgagca tactgatgaa aacggccgca ccgaaatcat caaaagtctc
     1801 tacaagagtt atatcaaggc tgccactgaa aagctcaaga gtgagcgtcc cgacttgctc
     1861 tcggtctttg aagacaatgc cggcgtggtg gccttcgatg aagagtatgc ggtctgcttt
     1921 aaagtggaaa cccataactc accctctgcg cttgagccat atggtggcgc cctcaccggt
     1981 attctggggg ttaatcgcga tatcctcggt accggccttg gtgccaaacc catcttcaat
     2041 actgatgtct tctgcttgcc tcacccggat tctccctttg tggccggtct taagcacgat
     2101 aaacttttga gcgctaagga aattgtctgc ggtgtgcgca aaggagtgca ggacggcggc
     2161 aacaagagcg gtattcctac cgtcaatgga gcggtcttct tcgacgattc ctaccgggcc
     2221 aagccgctgg tctactgcgg cacgggcggt ctcttgcccc tggaagcggc agggcgagac
     2281 gccgtcaaaa agcatacggc taaaggagac tatattgtaa tggccggcgg cagggtcggt
     2341 cgtgacgggg tgcacggcgc taccttctct tcccttgctc tcgatgataa taccggttcc
     2401 gatgtggtgc agataggtga tcccttcacc cagaagcgtc tgctcgactt tgtgctacag
     2461 gcccgcgacg gcggtcttgt caccggtctt accgacaacg gcgccggcgg gttgtcctca
     2521 tccgtcggtg aaatggccag tatcacaggt ggtgcgctgc tggagattga ttccgttccc
     2581 ctcaagtatc cggggctctt cgactggcaa atcgtggttt ccgagtcgca agagcgcatg
     2641 actttctcca ccgacaaacc cgacgagcta ttggctctgg ccgccaagct ggatgtggaa
     2701 gctaccgtgg tggggcgctt caccgatgac ggctacttcc gggtggtgcg gggcggtgct
     2761 accgtggccc ttctcaatct ggatttcctc catggcggtg cgcccggtct caaactgaag
     2821 tcgcgctggc agccgccggc tatcaatgcc gctaagccag aagtggcagc gaccggtcgg
     2881 caactcctct ccttactggc caatcccaat atttgcagcc gcgagcctgt tattcggcag
     2941 tacgaccatg aagtgcaggg cggtagcgtg atcaagccgc tcatgggccc ccgacagatt
     3001 tctccctgtg acgccggtgt gctcttgccg cgcccgtccg agaaaaaggg catagc
//
LOCUS       DAOQNZ010000341         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16677_length_3052_cov_3.975642, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000341 DAOQNZ010000000
VERSION     DAOQNZ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16677_length_3052_cov_3.975642"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PKW73_07310"
     CDS             complement(<1..1378)
                     /locus_tag="PKW73_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73129.1"
                     /translation="MTEEEYRPDDNLEAPEKNAEEPQPRQKTRRQSYAPSLNKFEKLF
                     SVTGFPPPRDNLDPNKKKITQLEWLMPDYESESAGEAKEIKGLAEELESAAGVQKRQE
                     AEKPAPKPIDPTHGYLPAPKPVPPPAPPPAPMPPSEIPELPRSLHVEIQRPAPEKPAE
                     PVPRQSGERPAQDPSGEGYSREALAKIPTPKNTGEAPKPSTRTRSEVPKQQPVEAPPE
                     PRRVSRLPKSFAAPEEDSDTAMDQNKRKLSAARASRPPSRFSHLLESHNGKIAAIAGG
                     SILAGFTIGIIAFLIMPKKESVESTSPAKPVQTVKKPALTLPFVKPQYKTTEEAEAAA
                     DTALLSGNLQEAMDALNQGLKINAGSKSLMQKRGLTYLLQKQYTEAQHDLAEVLTASP
                     RFLPALFDRAALSFAQEHYKEAQSDYNLILAIEPGNADALLGLSLCKFKQKDSEAGLT
                     TLSRITTRE"
     gene            complement(1418..1831)
                     /locus_tag="PKW73_07315"
     CDS             complement(1418..1831)
                     /locus_tag="PKW73_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73130.1"
                     /translation="MTTKSLATIVFIICLTFATCGQEASARRAYSSYGDVDEKVLDEI
                     LHGKKPAAQKKTTRPRSKSKPNQAPNTTGGAITIAPIEASASNGRRNSGRTTPTASGP
                     GEAQRKSLLSKLSPVPEMAPPTSLDMVKARQTKHQ"
     gene            complement(2018..>3052)
                     /locus_tag="PKW73_07320"
     CDS             complement(2018..>3052)
                     /locus_tag="PKW73_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73131.1"
                     /translation="PEQCLEQEVEPSSDIYALGCIMYETLTGKPALAARTIFETMNKH
                     LTTMPEPLAQVRPDLTFPDGLEKIIFKAMAKNPRNRFQSATELQAALKKLTLKVSSRH
                     DSKKAATEKAAVRESTTPPSSTEAALVLNFEPQPDITSLQSQAGDRLERPYIIHNPST
                     TAIPLTFFDLFTIIASLWFLFRIIFRGDSFNDLAPCLLMAACAPRLIYYAFILMMRSF
                     YHRRRLTNLASSLPVENIALLSRKTLRSQIFWAGTSQLPMSSASAPEIGLRVRTTEGP
                     REKYAMVITASEQHRDFWQSLTGRTGDTKHGKSPLPLGADIILDPQGFQIGIQYGQYF
                     AKIIKSYKLP"
BASE COUNT          543 a          717 c          936 g          856 t
ORIGIN      
        1 gctctcttgt ggtgatgcga ctgagggtag ttagaccggc ttcgctgtct ttttgcttga
       61 acttgcagag actgagacca agcaaggcat cggcgttgcc cggttcaata gccaggatca
      121 agttgtagtc gctttgagct tccttgtaat gctcctgggc aaaggagagc gccgctcggt
      181 cgaagagcgc cggcagaaat cttggactgg cagttagtac ctcagccaga tcgtgctgag
      241 cttcggtgta ttgcttttgc aaaagatagg tcaagccgcg cttctgcatg aggctcttgc
      301 tgccggcatt tatctttagc ccctgattga gcgcgtccat cgcttcttgt agattgccgg
      361 aaaggagcgc tgtgtcggcg gctgcttccg cctcttccgt ggtcttgtac tgaggtttga
      421 caaagggcag ggtgagggct ggtttcttga cggtttgcac tggttttgcc ggactggtac
      481 tttcgacgga ttctttcttg ggcatgatca ggaaggcaat tatgccgatg gtgaagcctg
      541 ccagtatgct accgccggcg atggcggcaa ttttgccgtt gtggctttcc aggaggtgtg
      601 agaaccgcga tggcgggcgg cttgctcttg ctgccgaaag ttttcttttg ttttgatcca
      661 ttgctgtatc gctgtcttct tctggggcgg caaaagactt aggtaaacgg ctgacccttc
      721 gcggttccgg tggggcttcc acaggctgtt gtttaggcac ttcgctgcgg gtgcgggtag
      781 atggtttggg ggcctccccg gtatttttag gagtgggaat cttagccaga gcttcacggg
      841 agtagccttc tccggaaggg tcctgcgccg gtctctcgcc tgactggcgg ggcacgggtt
      901 cggctggctt ttcaggagcc ggtctttgta tttccacatg cagggaacgt ggtaactccg
      961 gaatttcgga cgggggcatg ggcgccggtg gtggtgccgg cggtggcacc ggttttgggg
     1021 cggggagata accgtgggtg ggatcgatgg gtttgggcgc cggtttctct gcttcctgac
     1081 gtttttgcac gccggcggcc gattctaact cttcggccag ccctttgatt tctttcgctt
     1141 cgccggccga ctcactctca tagtctggca tgagccattc caactgagtg atttttttct
     1201 tgtttggatc taggttatct ctgggcggag gaaaaccggt aacggaaaat agtttttcga
     1261 acttattgag gctgggggca tagctctggc gtcttgtctt ctggcgcggc tgaggctctt
     1321 cggcgttttt ttccggagcc tcaagattgt cgtcgggtct gtattcttct tctgtcacgc
     1381 ttgcctgatt cgtttgctaa ctgaaagtgt ttgggcgtca ttggtgtttt gtctgtctgg
     1441 cttttaccat gtccagggaa gtcggcggtg ccatttccgg tacgggagaa agctttgata
     1501 gcaggctttt gcgctgggct tcaccaggtc cgctggcggt cggtgtggtc ctgccggaat
     1561 tcctgcggcc gttcgatgct gatgcctcaa tgggggcgat ggtaattgca ccgcccgtgg
     1621 tattgggtgc ttggtttggc ttgctcttgc tgcgggggcg ggttgtcttt ttctgcgccg
     1681 ccggtttctt gccgtgcaag atctcgtcta gaactttctc gtcaacatca ccgtagctgg
     1741 aataggcgcg ccgcgccgac gcctcttgcc cgcaagttgc gaaggtgagg cagatgatga
     1801 aaactattgt ggctaggctc ttggttgtca cttttgtcga tgtctgtcag gcgagaatag
     1861 cacgggccta ttagttttac ccagttagcc ccaaaacgtt gcacaattat cagacctcca
     1921 agtctggtgc atcgctttcg gctaatggta tttatcgaca gaactgcccc ccccggacaa
     1981 tattcccatt ctgcttgatt ctgagccaat gggccagtta tggcagtttg tacgacttga
     2041 tgattttggc gaagtattga ccgtattgaa tgccgatttg gaaaccttgg gggtccagaa
     2101 taatgtcggc accaagagga agtggtgatt tgccgtgttt tgtatcgcct gtccggccgg
     2161 tcaggctctg ccagaaatct ctgtgctgtt cactggctgt gatgaccatg gcgtatttct
     2221 ctctgggccc ttccgtagtt cttacacgca gtccaatctc cggcgcactg gcggaggaca
     2281 tgggcagctg gcttgttccg gcccagaaga tctgactgcg cagagtcttt ctcgagagca
     2341 gggctatatt ctctacgggc agtgaggaag ccagattggt gagacgtcgg cggtggtaaa
     2401 agctgcgcat cataagaatg aaggcatagt agattagccg cggtgcacag gccgccatca
     2461 acaagcaggg cgccaggtca ttgaaactgt cgcccctgaa gataattcta aaaaggaacc
     2521 agagggaggc aatgatcgtg aagagatcaa agaaggtaag cggaattgct gtcgtgctcg
     2581 ggttgtggat tatgtaaggc ctttccaggc gatcgccggc ttgtgattgc agcgaagtaa
     2641 tgtccggctg tggctcgaaa tttagtacca gggcggcttc ggtggaagag ggcggcgttg
     2701 tggattctcg caccgctgct ttttctgtgg ctgccttctt cgaatcatgc cgagaagata
     2761 ctttcagggt gagctttttc aaggctgctt gcagttcggt agcgctttga aaacgattgc
     2821 gagggttttt agccatggcc ttgaagatga ttttttcaag cccatccgga aaagttagat
     2881 ccggtctcac ctgggccagg ggctcaggca ttgtagtgag atgtttattc atggtttcga
     2941 aaatggttcg ggctgccagc gccggtttgc ctgtcaatgt ttcatacatg atgcagccga
     3001 gagcgtaaat gtcggagctt ggctctacct cttgctccaa acactgttcg gg
//
LOCUS       DAOQNZ010000342         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16700_length_3050_cov_3.840735, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000342 DAOQNZ010000000
VERSION     DAOQNZ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16700_length_3050_cov_3.840735"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="PKW73_07325"
     CDS             <1..1722
                     /locus_tag="PKW73_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNA73132.1"
                     /translation="AVRKSGITLAPEAGSEKLRAVINKGLKHDQIINAIESAYESGWQ
                     SVKLYFMCGLPFEDDEDLAGIIQILKEATNHARALRRSKPERYRRDIELTCTISNFVP
                     KPFTPFQWFPQVAPEEFARKHQVLRDKLRASGLRNVQLNVTGPQISLLEAVISRGDRR
                     IGEMILEAYKRGAVFDAWDEHFKPELWHQVAEDMGTTLLAEAAADRPVGSMQPYDIVH
                     IGLHHWWLVREWEKAVKEVETAACTENTCHACGICTELETNHVLASPLPAALKKNPFV
                     KELNANKSDPDSHPSLFFLAPKVPVGAVQAEDAAASPETANLRFRLVFQKVGDLKFIG
                     HLDLQNLFIRAFRRAGLEMSYTQGFNPSPKLSFAAPLPLFQEGLKELADIELASPVDA
                     HSLTFKLNRELPPEVQILECHELKEVAGGKKSLASLLGRAKYMALPQFDREMNEADRA
                     LTVSEMTAKFESAIEKIMGQEHLLAQMSAPPGKVGSTKDLRPLIHAISVHRQAVDFMS
                     AENAPWLELTLATGSNGHLKPQDLLAIISPDVTFRVIRLELLSQSGESLVPESAKENL
                     ELVRGSV"
     gene            1719..>3050
                     /locus_tag="PKW73_07330"
     CDS             1719..>3050
                     /locus_tag="PKW73_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNA73133.1"
                     /translation="MRKSIVISEQDNIAALLENDRVVEFFVNRGELLLGDIYTASVEN
                     ILPGIDAAFVNLGKDKMGFLHANDVPGQGPLKERLVPKQKLLVQITKEPTGHKGPRVS
                     TAISLPGRFLVLVPEERGVSISRRISEARERARLKAVVNLMKPPGVGLIIRTEAKGQG
                     DQELFEDFEQLWDRWQTIVSEAEASIGPTLIYRDQDLLFRVIRDAYSHEVTEIIADSP
                     EGQRRAQEYLAGWAHKQTKVIFHTADEPLLLAKGIDKEIEAALSNRAELPSGGHLVIQ
                     PTEALTVIDVNSGRFTSSRTQAETVRRTNLEAAQEIPRQLRLRNIGGMVIVDFIDMDS
                     RKDQQQVLEAFQRSLEEDRAKPQIGQLSDLGLVELTRRRQGQSLRELFTRPCTSCHTH
                     GVIPALELGPPEDRRIIAFRSKEEESVSRKNRGRNRSQPLKSATAVSGRVMP"
BASE COUNT          722 a          810 c          821 g          697 t
ORIGIN      
        1 gctgtccgta agagcggcat taccctggct cccgaagctg gttcagagaa gctgcgcgcc
       61 gtcatcaata agggtctcaa gcacgatcag ataatcaacg ccatcgagtc cgcttatgaa
      121 tctggctggc agtcggtcaa gctttacttt atgtgcggtc ttcccttcga agacgacgaa
      181 gacctggccg gcatcattca aatattgaag gaagccacca atcatgccag ggccctgaga
      241 cgcagcaagc cggagcgtta tcgtcgggat atcgagctga cctgtacgat ttccaatttc
      301 gtaccgaagc cctttacgcc cttccagtgg ttcccgcagg tggcaccgga ggaatttgcc
      361 cgcaaacacc aggttttacg cgataagctg agagcttcag gcttgcgcaa tgtgcaattg
      421 aatgtcacag gaccgcagat cagtttgctg gaagcggtta tctcccgcgg cgacaggcgc
      481 attggtgaaa tgatcctgga agcctataag cgcggcgccg tatttgatgc ctgggacgag
      541 cactttaaac ccgagctctg gcatcaggtg gctgaggaca tgggcaccac gcttttggcg
      601 gaagcagcgg ccgataggcc cgtgggcagt atgcagcctt atgacatagt gcatatcggg
      661 ctccaccact ggtggcttgt cagagagtgg gaaaaggccg ttaaagaggt tgaaaccgcc
      721 gcctgcacag agaatacctg ccatgcttgc ggcatttgca ccgaacttga aaccaaccat
      781 gtgctggcca gtcctttgcc tgccgccttg aagaaaaatc cttttgtgaa agagttgaat
      841 gctaataaga gtgatcccga ctctcatcct tcgctcttct tcctggcacc gaaagtgcct
      901 gtaggtgctg ttcaagcgga agatgccgcc gcttcgccgg agaccgccaa tcttcgcttc
      961 cgcctggtct ttcagaaagt gggtgacctg aaattcatcg gtcaccttga tctgcaaaat
     1021 ttattcattc gcgcttttcg cagagcggga ttggaaatga gttatacaca agggtttaat
     1081 ccctccccca aactttcttt cgctgcccca cttcccctgt ttcaggaagg gctgaaagag
     1141 cttgccgata ttgaattggc gagtccggtt gatgcgcact ccctcacgtt caagttaaac
     1201 cgtgagttac cgccggaagt tcagattctt gagtgccacg aattgaaaga ggtagcagga
     1261 ggcaaaaagt ctcttgcatc acttctgggc cgggctaagt atatggctct gccacagttt
     1321 gatagagaaa tgaacgaggc tgaccgagct ttgacggtat ctgaaatgac cgcgaagttt
     1381 gagtcggcga tagagaagat aatggggcag gagcatctac ttgcacagat gtccgctcct
     1441 ccgggaaaag ttggttctac taaggacttg cgtcccctca ttcacgctat ttcagttcat
     1501 cgtcaagctg ttgattttat gtccgctgaa aatgctccct ggctggagct aactctggcg
     1561 acaggctcga atggacattt gaagccgcag gatttgctgg ctatcatcag tcccgatgtc
     1621 accttcaggg tgatcagact tgaactttta tcccagtctg gtgagtctct cgttcccgaa
     1681 tcggccaaag aaaaccttga attagttaga ggatccgtat gagaaagtca attgtaattt
     1741 ctgagcagga caatattgcc gccttgctgg aaaacgatcg agtagtggag tttttcgtca
     1801 acagaggcga actcttgctc ggtgacatct atactgcttc ggtggaaaac attctgcccg
     1861 gtatcgatgc tgccttcgtt aatctgggca aagacaagat gggcttcctg cacgccaacg
     1921 acgtgcccgg acagggaccg ctcaaagaaa ggcttgtgcc caagcagaaa ctgcttgtgc
     1981 aaatcactaa agagcccacc ggacacaaag gtccccgggt atcgacagcc ataagtttgc
     2041 ccggtcgctt tctggtgctt gtgccggaag agcgcggcgt cagtatttcg cgccgtattt
     2101 cggaagctag agaaagagcc cgcctcaagg ccgtggtcaa tctcatgaag cctcccggcg
     2161 tcggtctcat catcagaacc gaagccaaag gacagggcga ccaggaactc ttcgaagatt
     2221 tcgaacagct ctgggatcgc tggcagacca ttgtttcgga agccgaggct tcgattggac
     2281 cgactcttat atatcgcgac caggatctgc tcttccgtgt catccgtgat gcttattccc
     2341 acgaagtaac tgagattatc gccgacagtc cggaaggcca gagaagagcc caggaatacc
     2401 tggccggttg ggcccacaaa cagaccaagg tgattttcca tactgccgat gagccgcttt
     2461 tgctggctaa gggcatcgat aaggaaatcg aggcggccct ctccaaccgt gctgagttgc
     2521 catccggtgg acacctggtt atccagccca cggaagcgct gacagttatc gacgtcaact
     2581 ccggtcgttt caccagttcc cgcactcaag ctgaaacggt acggcgcacc aatttggaag
     2641 cggctcagga aattccgcgc cagctcagac tgcgcaacat cggcggcatg gtcattgtgg
     2701 acttcatcga tatggacagc cgcaaggatc agcagcaggt gctggaagcc ttccagagat
     2761 ctctggaaga agaccgggct aagcctcaga ttggtcagct ttccgacctg ggtctggtgg
     2821 aattgaccag acgcaggcag ggtcagagtt tacgtgaact gttcacgaga ccctgcacaa
     2881 gctgccacac tcatggcgtc attccggccc ttgaactggg accgccggaa gatcgtcgca
     2941 tcattgcctt ccgttccaag gaagaagagt cagtctcgcg caagaatcgc ggtaggaatc
     3001 gctcccaacc ccttaaatcg gctaccgccg tcagtggtcg ggtgatgccc
//
LOCUS       DAOQNZ010000343         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16705_length_3050_cov_3.008681, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000343 DAOQNZ010000000
VERSION     DAOQNZ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16705_length_3050_cov_3.008681"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PKW73_07335"
     CDS             complement(<1..1289)
                     /locus_tag="PKW73_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA73134.1"
                     /translation="MSDLLELRAAFDKGLVSDGGGSVRYLLIEAKAREPLDLSCYDGD
                     SPCDMNMKAGAALNLVLVMDASGSMSGARIAEAKRAASQIVKGLGSRDYISVVSFAED
                     VIVHVDGRLCDSNGKRAVLSAIKDIEIRGSTNLCEGWLTGARCAGRVMEKRSGLKSHV
                     LVLSDGRANFGTTDPGVLAVHAQQLQIRGLTTSAIGIGEDYSTEQLYTMAVHGGGVVH
                     HTPNSDELVAVVCGELEGLRRRAVENLELTISYPQEVTVELLSFLPCEFANKGKLMVN
                     LGGISSQACRSLVLRVKVAEQYDEQRGLIKFGIVGHYRQSNGEEAELSTRPLQVSLRY
                     ADYDLSSTQAVNKTEALKIAEIWQSWLIWETCEMNRRRNYQKIDRFLGREERYMKRFC
                     GSLAEAQPLLKEVKELRRQACIDWDEASRKEIQSSSYR"
     gene            complement(1286..2008)
                     /locus_tag="PKW73_07340"
     CDS             complement(1286..2008)
                     /locus_tag="PKW73_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA73135.1"
                     /translation="MSEATFTLEELAEESGFDKRVIRSFIENGLLLGPDSRGRYARYG
                     PGHLTRLKAIRNLRERQSLSFNEVRQALMGMSDEDIRTLAEPPVSPRFRGNRRSSVID
                     YLDGVRAGLSGQTRDYVACTADDDDDQSGTLRGAPPREQDATLAGTSRKVEPEHGGED
                     EPPPEARNVSGSPVDRLLTALSSLTGSSGGKGQSRSEVLHRFSVTPDIEISIRNLSDA
                     SQIERWGRICDSLREILLGGGQ"
     gene            complement(2209..>3050)
                     /locus_tag="PKW73_07345"
     CDS             complement(2209..>3050)
                     /locus_tag="PKW73_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNA73136.1"
                     /translation="YVDFNGNRSFAAASMIKVPVLVALFSAIDRGDVDPKKILEIKTE
                     HVAGGSGYLQYRPVGTKLPLMQVAELMIIISDNTATNMIIDLLGGKEQVNKKFTAWGL
                     KNTVINNPLADLTGTNRTSPYDLVYLLARVERGDLMSDTWRQRMRKIMERTKTRTLLN
                     QGLPPGAKLAHKTGDIGSMVGDTGVVTTVDGRKYIVAVQVQRPHNDRRANELIRKLSG
                     IIYTAFASQPAALKPQPAALAQPSQLGQGEPAAGKVEGGATAGVSEGAVSPVSEPTHD
                     MSH"
BASE COUNT          712 a          901 c          796 g          641 t
ORIGIN      
        1 cgataggagg aagactgaat ctccttgcgg ctggcttcat cccaatcaat acaggcctgg
       61 cgtcgcaatt ccttcacttc cttgagcaag ggctgggctt cagccaggct gccgcagaaa
      121 cgcttcatat aacgctcttc ccgtcccaga aagcggtcaa ttttttggta attgcgccga
      181 cggttcatct cgcaagtttc ccaaataagc caggactgcc agatttcagc aattttaagg
      241 gcttccgtct tattcaccgc ctgggtggaa gaaagatcgt aatcggcata gcgaaggctg
      301 acttgcaggg gtctggtgct cagctccgct tcctcaccgt tgctctgccg ataatggccg
      361 acaataccaa atttgataag ccctctttgc tcgtcatatt gctccgccac tttcacccgt
      421 aaaaccagag aacggcaggc ctggctagag attccgccca ggttgaccat cagcttgccc
      481 ttatttgcaa actcacaggg caggaaagac agcaattcaa ccgtcacttc ttgcgggtag
      541 gagattgtca gctccaaatt ctccacggcc cggcgcctca gcccctccag ctcaccgcac
      601 accacagcca ccagttcatc ggaattgggc gtatgatgaa ccactccgcc accatgaacg
      661 gccatagtgt aaagctgctc cgtagaataa tcctcgccaa tccctatggc cgatgtggtc
      721 aacccgcgaa tctgtagttg ctgggcatgc accgccagca ctcccgggtc ggtggtacca
      781 aaattggctc taccgtcgga aagcaccagc acatgactct tcaatccgct tcgcttttcc
      841 ataacccgac cggcacagcg ggctcccgtc agccagccct cacagagatt ggtggagccg
      901 cgaatttcaa tgtctttaat tgccgagagc acggcccgtt tcccgtttga atcacaaaga
      961 cgaccgtcca catgaacgat cacatcttcg gcaaaggaga ccacggaaat gtagtcacgg
     1021 ctgcccagac ctttcacaat ctgggaggcg gcgcgcttgg cctcggcgat gcgcgcaccg
     1081 ctcatggaac cggaagcatc catgaccagc accagattca gtgcggcacc ggccttcata
     1141 ttcatatcgc agggggaatc gccgtcatag caagacaaat ccaggggctc ccgggccttg
     1201 gcctcgataa gaaggtagcg caccgacccg ccgccatcgg aaaccaagcc tttgtcgaat
     1261 gcggctctca attctagtaa gtcgctcact gtccacctcc aagtagaatt tcccgcaaag
     1321 aatcgcaaat acggccccac cgctctatct gagaagcatc agagagattg cgaatggaaa
     1381 tttcgatatc gggagttaca ctgaatctat gcaaaacttc gctgcgagac tgccctttac
     1441 ccccggaaga accggtgagg gaactaagcg ctgttaagag tcgatccacc ggtgagccgc
     1501 tcacatttcg cgcttccgga ggcggctcat cttcgccgcc gtgttcgggc tcgactttgc
     1561 ggctggtacc ggctaaggtg gcatcctgct cccgcggcgg ggctcctctc agagtgccag
     1621 actgatcatc gtcgtcatcc gcagtacagg cgacatagtc tctggtctgg ccggaaaggc
     1681 cggcccgcac accgtccaga taatcaatga cggaagagcg acggttgccc ctaaatctgg
     1741 gtgaaaccgg cggttcggcc agggtgcgaa tgtcttcgtc ggacataccc atgagggcct
     1801 ggcgtacttc gttaaaactc aaggactgcc gctctcgcag attgcgaata gccttgagcc
     1861 tggtcagatg acccggccca taacgggcat agcgaccgcg cgaatcagga ccgaggagca
     1921 agccgttttc aataaagctt cggatgacac gcttatcaaa accgctctct tctgccagtt
     1981 cttccagggt aaaggtagct tcgctcaagg ctagggcctc ataaataagg agagaaaaca
     2041 caacaagatc tatgcttgcg cccgcgacac aaacaataag agcacaaagc agcagccccc
     2101 gaggcaacgg cccgggcgca cactaaatag gaaggaaaga aaccacagtc gaactatcgg
     2161 actacttcta acacagtagc accttcaatt cgccatgtca aattgacttc aatgagacat
     2221 gtcatgagtc ggttcggaca ccggactgac cgccccttcg cttacaccgg cagtggcacc
     2281 gccctctact tttccggcag caggctcgcc ctgaccaagc tggctgggtt gagccagggc
     2341 ggcaggctga ggcttgaggg ccgccggctg ggaagcaaaa gccgtatata tgatgccgga
     2401 gagcttgcga atcaattcgt tggcacggcg atcattgtga gggcgctgca cctgcaccgc
     2461 cactatatat ttacgcccat ccaccgtggt caccaccccg gtatcaccca ccatagaacc
     2521 aatatcgccg gttttgtgag ccagtttggc ccccggaggc agaccctggt tcaggagggt
     2581 acgagttttg gtgcgctcca taatcttgcg catgcgctgg cgccaggtat cggacatgag
     2641 atcgccgcgc tccacccggg caagcaagta gaccaggtcg taagggctgg tgcgattggt
     2701 gccggtgagg tcggccaggg gattattgat gaccgtattt ttcaatcccc aggccgtgaa
     2761 cttcttattt acctgttctt tgccgcccag caaatcgatg atcatattgg tggcggtatt
     2821 gtcggaaata atgatcatca gttcggcaac ctgcatcaag ggcagcttgg tgcccaccgg
     2881 acgatactgc agatagccgg agccgccggc cacatgctcg gtcttaattt ccagaatttt
     2941 cttaggatcg acgtcgcccc tgtcaatggc actgaagagt gccaccagga cgggcacttt
     3001 gatcatactg gcggcggcaa agctgcgatt gccgttaaag tctacgtagg
//
LOCUS       DAOQNZ010000344         3049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16718_length_3049_cov_3.690381, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000344 DAOQNZ010000000
VERSION     DAOQNZ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16718_length_3049_cov_3.690381"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3049)
                     /locus_tag="PKW73_07350"
     CDS             complement(<1..>3049)
                     /locus_tag="PKW73_07350"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNA73137.1"
                     /translation="ALVAVMLETPTGSIAGTIALEKKQFGVYSYNLKENKVYALVNGP
                     RNGPQVERGVWVNDDGSFRIDQLPVGEYILEVRAPGFARELVRGLYIEDGKVAKLPKP
                     VKLSLLSPSVNIASNSRVFTTKDKPNFWVNATGAAEAEVKVYKGDLLALLDAKTSQKW
                     GISLASDMSLYVDSSKKFENPFESASSGNRTEPVKVFKRKLVQDQSDSSHAEFAFDET
                     LPPGDYFTVCEVKDLFGKEGAKAITWFSVSDIGLVIKHAPGEAVVRAVDLTNLKGASG
                     VNVALFERVEGTSPQARVKAGTGADGIAHLPLTEELRKKLGFDVIVTGVKGAHRAYGG
                     YNYWRADSDLHKTYFYTDRPVYRLGQTVMFKGIARLMGENGLSNAGAGQNLAITVEDP
                     DNNAIQTLNLKTSKHGTFSGLIDIPADGKTGGYQVQIAYGDGHTSYQSFEVAQYRKPE
                     YQVEVEPLSDRVIMGEKGRAKVRATYYFGGPVAGARVKYSIYSSTDWASRYKLMDRPE
                     YCGFFDDWEGSDYESSSGDFLKEGFAVTDENGEAIVEFETTAPTMPASGPIGSIFQDK
                     KLKIEAEVTDISRLSVVSSGNVPESSGNFVLLVNPDSWVVRAGEALSVNIRALTYDSK
                     AVANKPVKVKVMRFPYDRIKEEYKPDQVVVEQTVTTDSAGRAHLQAAIGNQLPSDSYY
                     VVAEAQDDGNHKVVDTTSIWVAAGEKPFFFSEKEADKQPLTVKLDKKVYKAGDKARLV
                     ISGPFTGKEGMEALVGVEGYKLHELKVVPLTSSAQVVEVDIKPNYAPNFYVTVSVVGK
                     KRQFYSQEEPVMVSPDNHFLKLSIETDKERYKPGDDAVYTIRALTADGKPAAGAEVSL
                     GVVDESIYAIRPETAEDIRKFFYSRISNWVTTLCSFPEQYSGGPDKLEPRVRKDFKDT
                     AAWFPNLVTDAEGKAVVKFKLPDNLTTWRATVRGITAGCDVGASIAKVISTQDIIARL
                     ALPRFFAQGDQGLVTAVVHNYTKKSQTVKINLTMGSNLKTTLPL"
BASE COUNT          584 a          802 c          849 g          814 t
ORIGIN      
        1 caggggcagg gtggtcttaa gattgctgcc catggtcaga ttgattttga cggtctgcga
       61 tttcttcgtg tagttgtgga ccacggccgt gaccagaccc tggtcgccct gggcgaagaa
      121 acgcggcaga gccagtctgg caattatgtc ctgggtagag atcaccttgg ctatggaggc
      181 tcccacatcg caaccggcag tgataccacg cacggtggcc cgccaggtgg tcaaattgtc
      241 cggcagtttg aatttaacta cggctttgcc ttcggcgtcg gtgaccaggt tggggaacca
      301 ggcggcagta tctttgaaat ctttgcgcac ccgcggttcc agcttgtctg gtccacccga
      361 atactgttcg gggaaggagc agagggtggt tacccagttg gaaatgcggc tgtagaagaa
      421 tttccggatg tcttcagcgg tttccgggcg gatggcatag atgctttcat ctaccacgcc
      481 cagggacacc tccgctcccg ctgccggctt gccatcggcg gtcagggccc ggatggtata
      541 gacggcatcg tcgcctggtt tgtatctttc tttgtcggtc tcaatggaga gcttgaggaa
      601 gtgattgtca gggctgacca tgactggttc ttcctgtgaa taaaactggc gtttcttgcc
      661 taccaccgac acggttacgt agaagttggg ggcgtaattc ggcttgatgt ccacttctac
      721 tacttgcgcc gaagatgtta gcggcactac tttcagctca tgcagtttgt agccttccac
      781 gcccaccaat gcttccatgc cttccttacc ggtgaagggt ccgcttatta caaggcgagc
      841 cttatcaccg gctttgtaga ctttcttatc gagcttgact gtgagtggtt gtttgtcggc
      901 ttctttctcg gaaaagaaga acggtttctc accggcggct acccagatcg aggtggtatc
      961 gacgactttg tggttgccgt cgtcctgcgc ttctgccact acgtagtagc tgtccgaagg
     1021 cagttgattg ccgatggctg cttgtagatg ggcgcgtccg gcactgtcgg tggtgacagt
     1081 ttgctcaacc accacctgat ccggtttgta ttcttcctta atgcggtcgt agggaaagcg
     1141 catcactttc actttgaccg gtttgttggc aacagctttg ctgtcatagg tgagggcgcg
     1201 aatgtttacg gaaagtgcct ccccggctct cactacccag gagtccggat tgacgagaag
     1261 cacaaagttg cctgatgatt ccggcacatt gccgctggag accacggaaa ggcggcttat
     1321 atccgttact tccgcctcga ttttcagttt cttgtcctgg aagatcgaac cgatggggcc
     1381 gctggccggc atggtgggag cggtggtctc gaattcaaca attgcttcgc cgttttcatc
     1441 ggtaacggca aagccttctt tcagaaaatc accggaagag ctttcgtaat cgctgccttc
     1501 ccaatcatca aagaagccgc agtactccgg tctatccatg agcttgtagc gcgaggccca
     1561 gtcggtggat gaataaatgc tgtacttgac tctggctccg gccaccggtc cgccgaagta
     1621 ataagtggct cgcactttcg cccggccctt ttcacccatg atgactcggt ctgagagcgg
     1681 ttctacttcc acctgatatt ccggtttgcg gtactgagca acttcaaaag attgatagct
     1741 ggtatgtccg tccccgtagg caatctgtac ctgatatccg ccggtcttgc cgtctgccgg
     1801 aatgtctatc agtcctgaaa aagtgccgtg tttgctggtt ttcaggttga gggtctggat
     1861 ggcattatta tcgggatctt ccactgtaat ggccaggttt tgtccggctc cggcattgct
     1921 caatccgttc tcgcccatca atctggcgat gcctttgaac ataactgtct gacccagcct
     1981 gtagaccggt ctgtcggtat agaaataagt cttgtgtagg tcgctgtcgg cgcgccagta
     2041 gttgtagccg ccgtatgctc gatgggcacc tttgacgccg gtgacgatca catcaaagcc
     2101 cagtttcttt ctcaattctt ccgtcagggg caggtgggca atgccgtccg cgcccgtacc
     2161 ggccttgact cttgcctgag gggatgtacc ttccactctt tcgaagagtg ctacatttac
     2221 tcccgaggcg cctttcaagt tggtgaggtc caccgctctc accacggctt ctccgggtgc
     2281 gtgcttgatc accagaccga tgtcgctgac tgagaaccag gtaatggctt tggcgccttc
     2341 cttaccaaag aggtctttta cttcgcaaac tgtgaagtag tcaccgggcg gtaaggtttc
     2401 atcgaaggcg aattctgcat gggaggagtc gctctggtct tgcaccaatt tgcgcttgaa
     2461 gaccttcacc ggctcagtgc ggttaccgct gcttgcactt tcaaagggat tctcaaattt
     2521 cttggaggag tctacataga gggacatgtc ggaggcgaga gagatgcccc atttctggct
     2581 ggttttagca tcaagaaggg cgagcaagtc acctttgtag acttttacct cggcttctgc
     2641 cgcaccggtg gcgttcaccc agaaattagg cttgtccttg gtggtgaaga ccctgctgtt
     2701 ggaagcgata ttgacactgg gagaaaggag gctcagtttg acgggtttcg gtaacttcgc
     2761 tacctttccg tcttctatat atagtccgcg caccagctct ctggcaaagc cgggtgctct
     2821 tacttccagt atatattcgc ccacgggcag ttggtctatg cgaaagctgc cgtcgtcgtt
     2881 cacccagacg ccgcgttcta cctgggggcc gttacgggga ccgtttacca gagcataaac
     2941 tttgttttct ttcaggttgt aactgtagac cccgaactgt tttttctcca gggctatggt
     3001 gccggcaatg gagccggtgg gcgtttccag catcacggcg acgagagcg
//
LOCUS       DAOQNZ010000345         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16753_length_3046_cov_4.196590, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000345 DAOQNZ010000000
VERSION     DAOQNZ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16753_length_3046_cov_4.196590"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PKW73_07355"
     CDS             complement(<1..1182)
                     /locus_tag="PKW73_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNA73138.1"
                     /translation="MPKLSCGLLLSLILLAPVVLLQGCQAGSEGQEARKDRQRQFNEA
                     AHGTAVRRYLRNVQEHVKAQWKPSEEEASKTGVVFFKIDKEGKVKDLKVKVSTGDALL
                     DQRMLNAVKEASPLPKPPQILENEISLDFSFDMRILSSTSIFDDIDELKSMVIKATRS
                     LEKNPDDTASLKQRALACLAMSDVLSARSAVKDLTRLLEFEREDAELYLLRAQAFKLL
                     REKANFLSDARKAAELKPEVETRLTLVEALSENGDLTEALAKCEEALTFSPNDADALS
                     TKAFCHLQMHDYQAGIADAEKALAIEPEHGAAYAYRGDCYEALKQYDKALKDYTKSIK
                     FNPDDEYSYLRRARLYLQLSKFGPAVVDATSALEIEPELAEAYYYRACGNKALGLAAQ
                     AQ"
     gene            complement(1250..2926)
                     /locus_tag="PKW73_07360"
     CDS             complement(1250..2926)
                     /locus_tag="PKW73_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNA73139.1"
                     /translation="MVISNLRVPTTKQIRALESAWIKSVEETAATNWGQVLMEVAGRA
                     ASHCALSIWNENPGHVAIFAGTGNNGGDGMVVARYLNLWGIPASVFIVGEGEMTTAEA
                     LTNKKLLERLDIEIVQLSGQEDLEDALESIASGATVIVDAIFGTGLSRDVGGIHRTVI
                     DAINNSARPVLSIDIPSGINSDNGRIMGAAVRADQTVTFGYLKAGLLTFPGAEYAGQL
                     SLVDIGLPDFSETFVQQLNLDKDSADNGSQIWLTTCGAVQGIMPFRPTNSHKGTFGQL
                     LTIAGSLGMSGAGVMAAASALKIGAGLSYLSTARSLIVGLPTHEIVYKPLSETTAQSI
                     SQEAVAEVLPLIDGVQAVVLGPGLSQNHDTVSFVLSLIDIISKPCVIDADGLNALAQN
                     PEVLLGQAPRRFVLTPHPRELSRLTGQSVEVIQSDRIGAALAAARKFGSVVVLKGHYS
                     VIASPTGQVFINPTGNSGMATAGAGDVLSGMIGGLMAQGLPPFDAAVAGVYLHGKAGD
                     LAANRPELAGAQASLVATDILAHIPFALASLQAGDVSYLEERLLQSVSSL"
BASE COUNT          617 a          830 c          858 g          741 t
ORIGIN      
        1 ttgcgcctgg gccgcaagac caagggcttt gttgccgcag gctcggtaat aatatgcttc
       61 agccagttcc ggctcaattt ccagggcact ggtggcatcc accacggccg gtccgaattt
      121 tgacagttgc agatagagcc tcgctcttcg caggtaagaa tattcgtcgt ccggattgaa
      181 cttgatgctt tttgtgtagt ctttcagggc tttgtcatat tgcttcagcg cctcgtagca
      241 atcaccgcga taggcatagg cggcgccgtg ctccggttct attgccagtg ctttttcggc
      301 gtcggctata cccgcctggt agtcgtgcat ctgcaggtga cagaaggctt tggtgctgag
      361 ggcatcggca tcattgggtg aaaatgtaag agcttcctcg catttggcca gggcttccgt
      421 cagatcacca ttttcgctca gtgcttccac cagggtcagg cgggtttcta cttccggctt
      481 gagctcagcc gctttgcgag catcagatag aaaatttgct ttctccctca gtaatttgaa
      541 ggcctgggcg cgcagtaaat agagctcagc atcttctctt tcaaactcca gtagtctggt
      601 cagatctttc actgccgagc gggctgaaag cacgtcgctc atagccaggc aagccagggc
      661 ccgctgcttt agagaggcag tgtcgtccgg atttttttcc aggctgcggg tggcctttat
      721 caccatactc ttcaattcat cgatgtcgtc gaagatactg gtggacgaaa gtatcctcat
      781 gtcgaagctg aaatcgagac tgatctcgtt ctctaaaatt tggggaggct tcggcagagg
      841 cgatgcctct tttacggcgt tcaacatgcg ctggtcgagc aaggcatcgc ctgtgctcac
      901 ttttactttc agatctttga ccttgccttc tttgtcgatt ttgaaaaaga cgacgccggt
      961 cttgctggcc tcttcttcag agggcttcca ctgcgccttc acatgttcct gcacattgcg
     1021 caaatagcgg cgcacggcag ttccgtgtgc cgcctcattg aactgtctct gccggtcctt
     1081 gcgtgcttcc tgcccttcgc tgccggcctg gcagccctgc aacagcacaa ccggggcgag
     1141 caaaatcagg gacagtaaaa gcccgcaact aagtttcggc aaccactgcc tgcgaggttt
     1201 ccggcgcatt tcataaatgt gaaggggcgg cgctttcaag ggggtcagtc tataagctgg
     1261 agacggattg caagagtcgt tcttccaaat aggatacatc gccggcctgt aagcttgcca
     1321 gcgcaaaggg aatatgggcc agaatgtcgg tggccactag tgaagcctgg gctccggcca
     1381 gttccggccg attggcggcc aggtcgccgg ctttgccgtg caggtagact cccgccaccg
     1441 ccgcgtcgaa gggcggtaga ccctgggcca tgagaccgcc gatcatgcct gaaagaacat
     1501 cgccggctcc ggccgtggcc atgccggaat tgcccgtagg attgatgaag acttgtccgg
     1561 tgggggaggc aatgacggaa taatgccctt tcaaaactac taccgaaccg aactttcttg
     1621 cggcggcaag agcggcgccg atgcggtcgc tctgtataac ctctactgac tggccggtaa
     1681 ggcggctcag ttctctggga tgaggggtga ggacgaaacg gcgcggcgcc tgtccgagca
     1741 agacctccgg attttgagcg agggcgttca gcccgtcggc atcaataaca cagggcttgg
     1801 aaataatgtc gatgaggctt aaaacaaagc tgaccgtgtc gtgattctgg cttaaaccgg
     1861 ggccaagcac cacggcttgc acgccgtcaa tgaggggcaa gacctcggcc acggcctcct
     1921 ggctgatgga ctgcgcagtg gtttcagaga gaggcttgta tacgatttca tgggtgggca
     1981 gcccgacgat gagagagcgg gctgtgctca aataagagag cccggcgccg atctttagtg
     2041 cgctcgccgc cgccatcaca ccggcaccgc tcatccccag cgaaccggcg attgtgagca
     2101 attgcccaaa ggtgcctttg tgactgttgg tgggtcgaaa gggcataatg ccctgcaccg
     2161 caccgcaggt agtgagccag atttgagagc cgttgtcggc gctgtcctta tcgagattta
     2221 gctgttgcac aaaggtttcg ctgaagtcgg gcagaccgat atccacgagg ctgagctgcc
     2281 cggcatattc ggcacccgga aaagtaagca aaccggcctt cagatagccg aaggtgacgg
     2341 tctgatctgc tcttaccgcc gcccccatga tgcgaccatt gtcggagttg atgccggaag
     2401 ggatatctat agatagaaca gggcgggcac tgttattgat ggcatcgatt acggtgcgat
     2461 gaatgccgcc gacatctctg gacagtccgg tgccaaaaat ggcgtctaca attaccgttg
     2521 ccccggaagc gattgattca agggcatctt ccagatcttc ttgaccggag agttgcacaa
     2581 tttcgatatc caatctctcc agcagcttct tgttggtgag tgcttccgcc gtggtcattt
     2641 ccccttcacc aactatgaaa acgctggccg gtatgcccca gagattgaga taacgggcca
     2701 ctaccatgcc gtcgccgccg ttattgcctg tgccggcaaa aattgccacg tgcccggggt
     2761 tttcattcca gatggaaagg gcgcaatggg aagcggctcg cccggctact tccatcagta
     2821 cctgtcccca gttagttgct gccgtctctt cgacgctttt gatccaggcg ctttccaggg
     2881 ctctaatctg ttttgtggtg ggcactctca gattgctaat taccattggc ttacctttct
     2941 ttggatttga taaattgatg cttgccccgg ttttggccgg tgatttttat tctttttcca
     3001 ccaggaagca gcgcaccaca tgccccttgg acttttcttc cagggt
//
LOCUS       DAOQNZ010000346         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16756_length_3046_cov_3.367770, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000346 DAOQNZ010000000
VERSION     DAOQNZ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16756_length_3046_cov_3.367770"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="PKW73_07365"
     CDS             <1..1599
                     /locus_tag="PKW73_07365"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNA73140.1"
                     /translation="ELIAILSVALAGISILFSEPGLSGDISFLILAVSASILGNTQGW
                     RRLFSFAMAICYFGHYYSSSILLGSAARAEDRLFSALMVSFIWLSFSMGIGYYIRGSA
                     ADRSYSTTISYWNAACFSFGLFIFCKGLDPNILETILAAAGAVYMGMARWLRKREESV
                     LSTVHFLLGLSLINGAKAMRFSGLEVLTLDVMQIWLLMVLGLKYDIRAFRLVGAYLSC
                     TFIFEWLGDFNRTEYGALAIPAFGFRSIAHTKLALCALAAFSHIAVVAHKVSRTYEKF
                     SYGAANILYSLASIQIESSSNASLPLILQSCVNLVLGLKLSEALYSGLGFVLFCGAWS
                     VTLCSTGTWSTAAIGLAVVVFYAMFAIARSDFRSTKARVAGDLMLLAAYSGTTLLTLL
                     LWEKMNSTYLSLAFGLEGLLLLSAAFLLKELTFRTQAFGVIAILVGKLLLFDFLNRGT
                     VERIVSFLGAGVTLLLSSYVYGLGTNAFAVRPNVVKSEDETEIEAGPDVLEPAVLEPA
                     DTESGTTLDTSRVEADLLKDVNRA"
     gene            1864..2805
                     /locus_tag="PKW73_07370"
     CDS             1864..2805
                     /locus_tag="PKW73_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNA73141.1"
                     /translation="MTEKQNITEKPARAEESRSDSTSRILFDDIGSGKTIGKFTHADA
                     QACKTNPEEGAVPTLLPGHRSNGCEFKGMKDLPKDLKHLDFPDIYSKAAESTVRIDAT
                     MTDPHDKRGRFKQEGPAGTGAIIGKDTEKGECLVATANHVVNGGKEVKISDIKAVSAD
                     GKKYETETRYSEPKKDTAVIAVKTGADTEKVCKPFTPVQDFNDEAGKGKNLVALGFPN
                     GSRALYASPGKSEGITPMRQDMSPGNIRELGLEPNASQLKVINHIKGGQSGGPVVTPE
                     GRLAGLNQSGPDGYTRGSYAVPLDQKRVDELLAKAGK"
BASE COUNT          679 a          766 c          793 g          808 t
ORIGIN      
        1 gagttgattg ctatattgtc ggtggctctg gccggtatat caattctctt cagcgagcct
       61 ggtttgagcg gcgacatttc ctttctcatt ctggcagtct cggcttccat attaggtaat
      121 actcagggct ggcgcaggct tttcagcttc gctatggcca tctgctattt cgggcactat
      181 tacagcagta gcatcttgct gggctctgct gccagagctg aagacagact tttctcggct
      241 ctcatggtgt ccttcatctg gctctccttc agcatgggaa taggttatta cattagaggc
      301 agtgccgctg atcgttccta ttccaccacc atctcttact ggaatgccgc ttgcttttcc
      361 ttcgggctgt tcatcttttg caagggactc gatcccaata ttctggagac catccttgct
      421 gccgccggtg ctgtctacat gggcatggcc cgctggctca gaaagcgcga agagtcggtg
      481 ctttccaccg tacacttctt gcttggtctt tctttgatca atggagccaa ggctatgcgc
      541 ttcagcggtc tggaagtgct tactctggat gtgatgcaaa tctggctctt gatggttctt
      601 ggtctcaagt acgatatcag ggcctttcgt ctggtagggg cctatctctc ttgtactttt
      661 atcttcgagt ggttgggtga tttcaataga actgaatacg gtgccctggc cattcctgca
      721 ttcggcttca gaagtattgc tcataccaag ctggctcttt gtgccctggc tgccttcagt
      781 catattgccg tggttgctca taaggtcagt cggacctacg aaaagttctc ctatggcgcc
      841 gccaatattc tttatagtct ggcgtccatt cagattgaga gtagcagcaa tgcctctttg
      901 cccctgattt tgcagagctg cgtcaattta gtgcttggtt tgaaactctc cgaggctctc
      961 tattcaggtc tgggctttgt tctattctgc ggcgcctgga gtgtcaccct ctgtagcacc
     1021 ggtacctgga gtactgccgc catcggctta gccgtagtcg tcttttacgc aatgtttgcc
     1081 atcgccagaa gcgatttccg tagcacgaag gcacgggttg ccggcgacct gatgcttctg
     1141 gccgcctatt ccggaacgac cttgctgact ctgcttctct gggaaaagat gaacagtact
     1201 tatctttcac tggccttcgg tctggaggga ctgcttctcc tttctgccgc cttcttgttg
     1261 aaggaactga cttttcgcac ccaggctttt ggtgttatcg ccatcctggt aggcaaactg
     1321 ctcctgtttg attttctcaa tcgcggcacg gtggagcgca ttgtttcctt tctgggagcc
     1381 ggtgtgacgc ttttgctttc ctcatacgta tacgggcttg gcactaatgc ctttgctgta
     1441 agaccgaatg tagttaagtc tgaggatgag actgagattg aggcaggacc tgatgtcttg
     1501 gagcctgctg tcttggaacc tgccgacact gaaagcggca ccacacttga tacttccagg
     1561 gttgaggcgg atttgctgaa ggatgtgaac cgtgcctgaa attagtgttg atttcgcaag
     1621 aagctctggg tgcaggttca gtcagggcgc tactggtgaa ttgatgattt gatgaattgg
     1681 tgaattagcc tgcgttctga aaggaaagtg tcgctagaag tctacttttg ttgttaacga
     1741 ctcgattaca aaatctgact attctatgca ctaccacctc aatgcagttt tctacaccca
     1801 taagctccca gaataacccc gcacaggggt tggttcaggc tgagcattta ccaggttatc
     1861 catatgaccg aaaaacaaaa cattaccgag aaaccggccc gtgctgaaga gtctcgctct
     1921 gacagtactt cacgtattct ctttgacgac ataggtagcg gcaagacaat aggcaagttc
     1981 acccatgccg atgcccaggc ctgtaaaacc aatcccgaag aaggcgcggt tccgactttg
     2041 ctgcccgggc acaggagcaa cggttgcgaa ttcaaaggca tgaaagattt gcctaaagat
     2101 ctcaaacatc tggacttccc cgacatctat agtaaagccg ctgagagtac tgttcgcatt
     2161 gacgcgacca tgaccgaccc ccatgataaa agagggcgtt tcaaacagga gggaccggcc
     2221 ggcaccggtg caatcatcgg taaagatact gagaaaggag aatgccttgt cgctactgct
     2281 aatcatgtag tcaacggagg caaggaagtg aagatttccg atatcaaagc cgtcagcgcc
     2341 gacggcaaga agtatgaaac cgagacccga tactccgagc ccaagaagga cacagccgtt
     2401 atcgccgtga agacgggtgc cgacaccgaa aaggtctgca aaccatttac gccggtgcaa
     2461 gacttcaatg acgaagcagg gaaaggtaag aacttagttg cgcttggctt tccgaatgga
     2521 tctcgtgccc tttacgcctc accaggcaaa tcggagggta tcacaccgat gcgccaggat
     2581 atgagtcctg gaaatataag agagctgggg ctggagccaa atgccagcca gcttaaggtc
     2641 atcaatcaca taaagggcgg acagtccggt ggtccggtgg tcaccccgga aggacggcta
     2701 gccggtctca atcagtcggg accggatggc tacactagag gaagctatgc tgtgccgctg
     2761 gatcaaaagc gtgttgatga gctattggca aaagccggca aatagaaaat acataacgcc
     2821 tgtttgacta tttcaaaaag ctgtcataat tagtcctgat gaaactatct ggagtccccc
     2881 ggtgtctctg cgcggtctgg cgattgcctt gtgcctgttg gttttgcaac tgagttgcac
     2941 accccaggct gctcgcgccg gcgagaatgt cacagccaga attagagctt taagccatat
     3001 caaccgtccg gctcgggtgc tgagcttccc ggcggataag tcttgc
//
LOCUS       DAOQNZ010000347         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16812_length_3041_cov_4.145680, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000347 DAOQNZ010000000
VERSION     DAOQNZ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16812_length_3041_cov_4.145680"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKW73_07375"
     CDS             <1..471
                     /locus_tag="PKW73_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73142.1"
                     /translation="LLGIWEGSGNVICLDVLRAVAREPETLDAVTAELKLEVGKNRQY
                     DRFVGALEKSIAAFKKALASQSAGSTKSAGAGSKKKPSKKAMESAFATEAGARRLVEH
                     LALALQARMMLEQSTRESADAFIASRLGRSGYAFGTLDPARVDVKAIVDQAWLS"
     gene            1630..2931
                     /locus_tag="PKW73_07380"
     CDS             1630..2931
                     /locus_tag="PKW73_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA73143.1"
                     /translation="MFWKTFQLAVLALLLTLPATGRTAQTCDKPPPQAAPPPTYSGQP
                     ANDGYQIMYHSMWEFIRDSYFDPGRLKDWTKYEHAFDDKLGTIADIEIALKALAQATG
                     DKWTTYITAQDMRDHAAMEQQGLVTGGIMLYHRGQHYKLDVIHYGSAGYHTTLRERDD
                     ILCLNGVALDTLAPEQVAALLRGKSGDKLKVKAISSEDGQEFEVELTLAAIPAPAVQA
                     KLLQNGIAYLTMPSFAGEHFITEFVEQYSALVSQNGGKVNGLILDLRYNHGGELPAAT
                     KFSSLFLDESKVVTQSVLRDKPIKLIHANKADEISIKGKVVDATLVAQLRKLPLVILA
                     NGSSASAAEVTLAALKDNKRGVIVGDTSFGKGVGYKTQRGPVGGLMSLTAFKYLSPNG
                     NEVHEIGITPDFPVAVPRSETRDVQLETAIIRLEEKLQNSP"
BASE COUNT          758 a          899 c          731 g          653 t
ORIGIN      
        1 cttctgggca tctgggaagg ctctggcaat gtcatctgcc tggatgtgct gcgcgccgtt
       61 gcccgcgagc cggaaacact ggacgcggta acggccgaac tgaagctgga agtaggcaag
      121 aatcgccagt acgaccgctt cgtcggtgcc ctggaaaaga gcatcgccgc cttcaagaaa
      181 gcccttgcca gccagagcgc cggatcaaca aagagtgccg gcgccgggtc aaagaagaag
      241 cccagcaaga aggccatgga aagcgccttt gccaccgaag ccggtgcccg ccgcctggtg
      301 gaacacctgg ctctggccct gcaagcgcgc atgatgctgg agcaatccac cagggaaagc
      361 gccgacgcct tcatcgcctc gcgcctgggc cgttccggct atgccttcgg caccctggac
      421 ccggccagag tggacgtgaa agccatcgtc gaccaggcct ggctgagcta gcgaactgac
      481 ggcaggcaag aacagtttcg attaagagga ccaggtggtt gccacctggt cctctccttt
      541 tttcggcaac taccggcaaa ccgtgccata gtcatatttt ccgaacttgc aaaaaacgcg
      601 cctgctacta tttctcttag tatttctcat cttgccgcaa cggtgagccg atggcagcgc
      661 cagtactttt gtggcgccca ggcgttagca ccgtcagttc ttttgcggcg tccaggcatt
      721 agcaccgtca gttcttttgc cgcgactaga caggagtaac gccctttctt ctgccggggc
      781 aagaagctcg taccatcctt ctttttcaaa tcacccggga aagcactaaa ctcatgtaac
      841 gagaaaaatt aaatcagctt gacggctaaa tgccagtccc tgtgctgatt tcattttgca
      901 aaaaacctcg aaaccctgat gcaatatttc gcaatttcac tattgttaca ttggcaacgg
      961 ttccgcaaag caactaccca agccggcttt ggcaacactt tcgttaatct gaggcggcga
     1021 gcaaaagcat cgactattca gcacagatac tcgacatctc tttgagcatc attcgcctct
     1081 gactctcgat aacgccatcc aacacgactt ctgaacgctt tcccgctaca tccatgagac
     1141 atccgtagcg tcgcaaactg cgaccggtct atcaccgaga cagaaagtac acctaacccg
     1201 taagcacatc ctgcccgatt gggaacctgt gggaaaacag ccgctaccca ctagcatcga
     1261 aggattccaa gcgacttgtg gcagcttcaa ggactttgct aaagcaggtt gtatatttct
     1321 tcttttctat ttctgaaggt cttccacccc aaatctttcg gcttcctcca tctccacaaa
     1381 tccggcatag aacatctcgc tgtctcctgt cagatagctt gatccagcct tagaaatcga
     1441 gatagccgac gagaaaatcc ttcactgcca ttctggtaat ccgcagagag agtttgaaag
     1501 atatcccggg agatttttga gagaacttct gggagcgctt cctgagaggg cttccgggtg
     1561 aactttcaga gagcctcccg tgcagcctca gcaattacca gcccctcact ttctgacatg
     1621 gagacaatca tgttttggaa aacatttcag cttgccgttc tggccttgct gcttacctta
     1681 cccgccaccg gccgtaccgc tcaaacttgc gacaaaccac cgcctcaagc ggcgccaccg
     1741 cccacttatt cgggtcaacc ggctaacgac ggttatcaaa tcatgtatca cagcatgtgg
     1801 gaattcatcc gcgatagcta cttcgacccc ggtcgactga aagattggac caaatacgaa
     1861 catgcatttg acgacaaatt aggcaccatc gccgatatag aaatcgctct caaagcactg
     1921 gcacaggcca ccggcgacaa gtggaccacc tacatcaccg cccaggatat gcgtgaccat
     1981 gccgccatgg aacagcaagg actggtaacg ggcggcatca tgctctacca ccgcggtcag
     2041 cactacaaac tggacgtaat ccattacggt agcgccggtt accataccac cctgcgggaa
     2101 cgagacgaca tcctgtgttt gaacggagtc gctctcgata ccctggcgcc tgaacaagtg
     2161 gctgccctcc tgcgcggcaa gagcggcgac aagctgaagg tgaaagccat ctccagcgaa
     2221 gacggtcagg agttcgaagt ggaattgacc ttggccgcta ttcccgcccc cgccgtacag
     2281 gctaaactgc tgcaaaacgg catcgcctat ttgactatgc cctcctttgc cggtgaacat
     2341 ttcatcaccg agttcgtgga gcaatactct gccctggtca gccaaaacgg cggcaaggtg
     2401 aacggtctca tcctcgatct cagatacaat cacggcggag aactgccggc cgccacaaaa
     2461 ttctcgtctc tcttcctgga tgaaagtaag gtggtgaccc aatcggtgct gcgcgacaaa
     2521 ccaatcaaac taattcacgc caataaggca gacgagatta gcatcaaggg caaggtagta
     2581 gatgccaccc ttgtagcaca attgagaaaa ttaccgctgg taatcctggc taacggctct
     2641 tcggctagtg cggcagaagt gacactggca gccttgaaag acaacaagcg cggcgtcatt
     2701 gtcggcgaca ccagcttcgg aaaaggcgtt ggttacaaga ctcagcgcgg accggtcggc
     2761 ggtttgatga gcctgaccgc tttcaaatac ctttccccga acggcaacga agtgcacgaa
     2821 atcggcatca ctcccgactt tccggtagcg gttccccgta gtgaaaccag ggatgtgcaa
     2881 ctggaaacag ccatcatcag actggaagaa aagcttcaaa acagtccgta attacaagac
     2941 agcacttgcc ctcccacagg caagtgctgc catacttctc gaagatttcc ttttcagtca
     3001 atctactatc ccttttcgta ctcccaactc gctccatttc c
//
LOCUS       DAOQNZ010000348         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16866_length_3037_cov_3.788062, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000348 DAOQNZ010000000
VERSION     DAOQNZ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16866_length_3037_cov_3.788062"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="PKW73_07385"
     CDS             <1..1455
                     /locus_tag="PKW73_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNA73144.1"
                     /translation="TTTIDVLRLACAINGGDVSLAKPTRFAQLKRPHRRLMLKALESC
                     SNLEEDLRRYKGRFLRLAERLHPGEFARLYPKTFAAFQGLRHNKKLETFNQQVELRFT
                     EGNLEQLLQVLKSRPGLFARRLDHLLRRFPEQNTTVIDAFEAVSDGITTAMLLQLRTH
                     FGERCKKDKAEKRVFLPKGQVTKAMAVDNQLADLDESVCLRLLVLCEKLLKARFAKLP
                     PLGRCYLSPALVNFNLPFALRSASKSFRTMARGSRLALPDKDTLRFFLFWRNGRGRAD
                     VDLSAVFFDKDFNYLDAVTYYNLKSYGGVHSGDIVDAPQGASEFIDVSISLLMEKKVR
                     YVVPVIKAFTHEPFCDLPECFTGWMARQKPGSGEIYEPRLVEDRLDITSDSRIAIPAV
                     FDLRERQLIWADLALTRWPLWITTVAANLWGIQLMLKSMVMLKKPNVYDLLKLHIEAR
                     GSFCDSRDGADHCFSVEDGTPFDLAKLAGEFMQN"
     gene            1486..>3037
                     /locus_tag="PKW73_07390"
     CDS             1486..>3037
                     /locus_tag="PKW73_07390"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73145.1"
                     /translation="MQRYFERSPVRRKGFALSSLFVLMCLAVSQSSALEPSQSSVVAK
                     CKEISKLNKAKHSREAERLADSLLRQYPQENRYRHEKIEILHDRLNYDAALKEIDVCL
                     ASNPRDFVAYYWQADILSHQEDFQAARIAVNKCIESNPRYAPGYELKSTFEYRERKHR
                     HALSYASLALKYDAKDAGAWNSRGLAYLGLGDVQAAIRDFRKAVELSPGATLYLNNLA
                     EALRDSGRVSEALEKLNQALKLEPDNYYILQKRARAYGDMGSIKLALNDINRVIAARP
                     DKVADDYVCRAYIYIRQARYADAVVDAKHALKVNPQFASAYYVIGVALYCQHKYKDAI
                     PYFTKVLELDQYIGLAHKVRGLCYKAIGEDEKALADLEQYTSLFHDEESYRARAAIAA
                     KQGYFEQAADDLAALTPFAGGLKTGKKDYNRLISLYSRMAKAGLGGKEVYFDRGMVYF
                     VAGDTELACRDFEQYLRQSGAKGRNALPAAVWAMLGYRKMKKEPQVVAVLDYLKGANP
                     GLLSSGELL"
BASE COUNT          707 a          760 c          826 g          744 t
ORIGIN      
        1 accaccacca ttgatgtttt gcgtctggct tgtgccatca atggcggtga cgtatctttg
       61 gctaagccga cacgttttgc ccagctcaag cgcccccaca ggcgactgat gctgaaagcc
      121 ctggagtctt gctccaatct tgaagaagat ctgcgcagat ataaaggtcg cttcttgagg
      181 cttgccgaga gattgcaccc gggagaattt gcccggcttt atccgaagac ctttgccgct
      241 tttcaaggtt tgcgccacaa caaaaagctt gagaccttca atcagcaagt ggaactgcgc
      301 ttcaccgaag gcaacctgga gcagttattg caagtattga aaagtagacc gggactgttt
      361 gccagaaggc tcgatcactt gttgcggcgc ttccccgagc aaaatactac cgtcatcgat
      421 gcttttgagg cggtttccga cggcattacc accgccatgc ttttgcaact gcggacgcat
      481 tttggggaac gctgcaagaa agacaaggct gaaaagcgtg tcttcctgcc caaagggcag
      541 gtgacaaaag ccatggccgt ggataatcaa ctggccgatt tggatgagtc tgtctgcctg
      601 cgtctactgg tgctttgcga gaagcttttg aaagctcgct ttgctaaact gccgccactg
      661 ggtagatgct atttgtcgcc ggcactcgtc aatttcaatt tgcctttcgc gctgcgctcc
      721 gcctcgaaat cctttcgtac catggctcgc ggcagccgcc tcgctctgcc ggacaaagat
      781 actctacgct tctttctttt ctggagaaat ggccgcggca gagcggacgt ggatctgtcg
      841 gcggtttttt tcgacaaaga cttcaactat ttagatgccg tcacctatta caatttgaag
      901 agctacggcg gggttcatag cggcgacatt gttgatgcac cccagggcgc cagcgagttt
      961 attgatgtat ccatcagtct attgatggag aagaaagttc gttatgtggt gcccgtgatc
     1021 aaagctttta cccatgagcc gttttgtgat ctgcccgaat gcttcaccgg ttggatggca
     1081 cggcaaaagc ctggttcggg cgaaatttat gaaccgcgcc tggtggaaga ccgccttgat
     1141 ataacttccg acagtcgcat tgccataccg gcagtctttg atctgcggga acggcaactg
     1201 atctgggcgg acctggctct gacgcgctgg ccgctctgga taaccacggt ggctgccaat
     1261 ctctggggca ttcaactgat gctcaaatct atggtaatgc tcaagaaacc caacgtttat
     1321 gatctcttga agttgcatat cgaagcccgg ggaagttttt gtgatagccg agatggtgcc
     1381 gaccattgct tcagcgtcga agacggcacg cccttcgacc tggccaaact tgccggcgaa
     1441 tttatgcaaa actagggctg tgccgtattc acgatggtat ctataatgca aagatacttt
     1501 gaacgttctc ccgtaagaag aaagggtttt gctctttctt ccttgtttgt gctcatgtgc
     1561 ttagctgtca gtcagtcttc agctctggaa cccagtcagt cgtctgtcgt tgctaagtgc
     1621 aaagagataa gcaagctcaa caaagcaaag cacagccgag aggcggagcg actggccgat
     1681 agcctgcttc gtcagtatcc gcaggagaat cgctacaggc atgagaagat cgaaattctg
     1741 cacgaccgcc taaattatga tgcggccctg aaggagatcg atgtttgtct ggccagtaat
     1801 ccccgggatt ttgtcgcgta ttactggcag gcggatatcc tcagtcacca ggaagacttt
     1861 caggcggcgc gcattgcggt caataagtgt attgagagta acccgcgcta cgctcccggt
     1921 tatgaattga agagcacctt cgagtacaga gaacgcaaac atcgccatgc cttgtcctat
     1981 gcctccctgg ctctcaagta cgatgcaaag gatgccggtg cctggaattc cagagggctg
     2041 gcttatcttg gtctgggaga tgtgcaagcc gccataagag acttccgaaa agcagtggag
     2101 ttgtcgccgg gtgcaaccct ttatctgaat aatctggctg aggccctgag agattcaggg
     2161 cgagtttctg aggctctgga aaaattgaac caggctctca aactggagcc cgacaattat
     2221 tacattctgc agaaacgggc acgggcctat ggagacatgg gttccatcaa gctggctctc
     2281 aacgacatca atcgggtcat tgccgctcgc cctgataaag ttgccgatga ctatgtttgc
     2341 cgcgcctaca tctacatccg ccaggctcgt tatgctgatg ccgttgtcga tgccaagcat
     2401 gccttgaaag tcaatccgca gtttgcaagc gcatattatg taataggcgt ggcgctctat
     2461 tgccagcata agtacaaaga cgcgattccc tatttcacaa aggtgctgga actggatcaa
     2521 tacatcggtc tggcccataa agtgaggggg ctttgctata aagccatcgg cgaagatgag
     2581 aaagccctcg ccgaccttga acaatatacg agcctctttc acgatgaaga gtcttaccgg
     2641 gcgcgagcgg caattgccgc caagcagggt tattttgaac aggccgccga tgatctggct
     2701 gccctgacgc catttgcggg cggtctaaaa acaggtaaaa aggactacaa caggctcatt
     2761 agtctttata gccgcatggc taaggccggt ctgggcggta aagaggtcta cttcgaccgg
     2821 ggcatggttt attttgtggc cggtgatacg gagcttgcct gccgtgattt cgagcaatat
     2881 ttgcggcaga gcggtgctaa ggggagaaac gctctgccgg cggctgtatg ggctatgctc
     2941 ggctatcgca agatgaagaa ggaaccgcag gtagtggcgg tgcttgatta cttgaagggg
     3001 gcaaaccctg gtctgctatc cagtggggag ctactgt
//
LOCUS       DAOQNZ010000349         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16872_length_3036_cov_4.547467, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000349 DAOQNZ010000000
VERSION     DAOQNZ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16872_length_3036_cov_4.547467"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKW73_07395"
     CDS             complement(<1..351)
                     /locus_tag="PKW73_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73146.1"
                     /translation="MKSENRPSVKTSPAGTKAKTWLKVSCSLLSLACLTVMIGVPVGA
                     SGPDAEKKQSASYRLQEQELLRLIGADDQSSLAATRAAMTLPDLDKLQNKDKTQPLTT
                     FEFYGQNLLALIDAP"
     gene            complement(502..1857)
                     /locus_tag="PKW73_07400"
     CDS             complement(502..1857)
                     /locus_tag="PKW73_07400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNA73147.1"
                     /translation="MLPIEQSCYWLATRPQSAQGEALIGAQRAQVVIVGAGFTGLWTA
                     HFLKSLKPDLDLAIVEQGVTGYGATGRNAGMISNCIDHTHSLAIAHFGQEEARELAAI
                     GLANINEMESYATDCDLERTGQLFVALKEKHLHDLHSLIEDAERLDIKGYQLFDQKEI
                     QTKLNSPRYAGGAHVPGGGILNPIKLVDKLVSDLKRSGVRIFEKTRVDSVHQGQIKAG
                     DASIQADKIILATDAYTHHLLPGLIWRFIPLYDYILVSEPLETSELEKLGWQGREGIV
                     DGRTFFNYYRLTADNRVLWGTSEAKYYAPNQVNERFDHSHEHYQSLKESFSWHFPQLK
                     PMQWPYAWGGPIASTTRLTPFFGTAHNGSVLYALGYTGHGIGSTRLAGKILAHMALEQ
                     PSTLLKLKMVTDKPMPYPPEPLRSLSVNMVTSSLRQVDQGAKPNLLLKILDALGIGFS
                     S"
     gene            complement(1920..2162)
                     /locus_tag="PKW73_07405"
     CDS             complement(1920..2162)
                     /locus_tag="PKW73_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73148.1"
                     /translation="MQETKYKTLASNTSNAHTAYCSLSLNKGAQALVRKGGMRFVREL
                     DLVLEEEKEMTIKNISIKSQAPYSTAPAFISSHEGA"
     gene            2221..>3036
                     /locus_tag="PKW73_07410"
     CDS             2221..>3036
                     /locus_tag="PKW73_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HNA73149.1"
                     /translation="MKRRKKQRKNVIAKQAVTCLVTGFDAFGGQKANPSSELVEAMPP
                     GLFYRQAPSLKKPQDVASVQPAQGKPFGKICSQTSAVRIKFAKKVLPTASRSGWRALK
                     QAIDTTLAGTDEPLVLVMTGLAASRDRLSLERFALNVRDYGMPDNEGELAVDTFVDPE
                     QPDLLRTMLPLPPIVRAVEQAGYPCQVSNHAGTYICNELYFKALSYCRRNSRVKAVVF
                     VHIPPLDKFAATAELYGSLAVSRQAFNAVANSSNSHLHQLDLMRHALETIVKAV"
BASE COUNT          608 a          772 c          856 g          800 t
ORIGIN      
        1 tggtgcgtcg atgagagcaa gcaggttctg accgtaaaat tcgaaggtgg taagaggctg
       61 ggtcttgtct ttattttgca gtttatccag gtccggtaga gtcattgctg ctcttgtggc
      121 cgccagactg gattggtcgt ccgcaccgat cagtcgcagc aactcctgtt cttgcagccg
      181 gtaactggca ctttgttttt tttcagcgtc agggccggac gctccgaccg ggacaccgat
      241 catgaccgtc aggcaagcca gagaaagcag gctgcaggaa actttcagcc aggtttttgc
      301 tttcgtgccg gcgggggacg tttttactga aggacggttc tcgcttttca tttttagctt
      361 tctctgggta gtaagttcct tgaccggaag gcggcttggc attttgcttt tcaccgcggt
      421 tttgcacagc ttgccttact tttgccgggt atatttttag aggcgttttg aacagttccg
      481 caagtgtttg ccaattcggc cttagctgga aaagccaatt cccagcgcgt cgagtatctt
      541 aagtaagagg ttgggttttg cgccctggtc tacctgtctg agggagcttg tcaccatgtt
      601 taccgagagg gaacgcaggg gttccggcgg gtagggcatg ggcttgtcgg taaccatttt
      661 tagcttgagc agggtgcttg gctgttcgag agccatatgg gccagaatct tgccggccag
      721 tctggtggag ccgattccgt gtcctgtata gccgagagca taaagaacgc tgccattatg
      781 ggcggtgccg aagaagggag taaggcgggt ggtagaggct atgggaccgc cccaggcgta
      841 gggccattgc atgggtttta actgcgggaa gtgccagctg aaactttctt tcaaggactg
      901 gtaatgttcg tgggaatgat cgaagcgctc gttgacctga ttgggtgcgt aatatttggc
      961 ttcgctggtg ccccagagca cccgattgtc agcagtgagg cggtagtaat tgaaaaaggt
     1021 gcgaccgtcc actatgcctt cccgcccctg ccagcctagc ttttccagct cggacgtttc
     1081 gaggggctcg gaaaccagaa tataatcgta gaggggaatg aaacgccaga tcagcccggg
     1141 tagcagatgg tgcgtatagg catcggtggc cagtattatt ttgtcggcct ggatggaggc
     1201 gtcgcctgcc ttgatttgac cctggtggac gctatctact ctggtcttct cgaaaatgcg
     1261 aacgccgctt ctttttagat cggaaaccag tttgtcgacc agtttgattg gattcaagat
     1321 accgccgcca ggcacatgag cgccgcctgc atagcgggga gagttcagct ttgtctgaat
     1381 ctctttttga tcgaagagtt ggtagccctt gatgtctagc cgctcggcat cttcaatgag
     1441 ggaatgcaaa tcatgcagat gtttttcttt caaggcgacg aaaagttggc cggtgcgctc
     1501 cagatcgcaa tcagtggcat agctttccat ttcattgata ttggccagtc cgatagcggc
     1561 tagctctctt gcttcttctt gaccaaaatg ggcgatggcc aggctgtggg tatgatcaat
     1621 gcaattgctg atcataccgg cattgcggcc ggtggcgccg tagcctgtga cgccctgttc
     1681 gacaatggcc agatcgagat cgggcttgag gctctttaga aagtgcgccg tccacagccc
     1741 cgtgaagcct gcccctacta tcactacctg ggccctttgc gcgcctatca aagcttcccc
     1801 ctgggcgctc tgcgggcggg tggctagcca atagcaagac tgttcgatgg gcagcatagg
     1861 caaagtctag cactgttagt agagcttgat ttccttaacc cctccggtag tggttattgt
     1921 caagcgcctt cgtgagagct aataaatgcc ggcgctgttg aatatggagc ttgggacttg
     1981 atagagatgt tctttattgt catctctttt tcttcctcta aaaccaggtc aagctctctt
     2041 acaaatctca tccccccttt ccttaccaat gcttgggcgc ctttattgag gctcaggctg
     2101 cagtatgcgg tatgtgcatt ggatgtgttt gaagcaagag ttttgtactt ggtttcttgc
     2161 aatcgctgtt cttgccgcct ctggcgcaag tcggctcata gcgcccagga gactttcata
     2221 atgaaacgta gaaagaagca aagaaagaat gtgattgcca agcaggcagt cacctgtctt
     2281 gttactgggt ttgatgcttt tggcgggcaa aaagccaatc ccagctccga attggtcgag
     2341 gcaatgccgc cggggctctt ttatcggcaa gctccgtccc tgaagaagcc gcaagatgtt
     2401 gcctccgtcc aaccggctca gggcaagccc ttcggcaaga tttgttcgca aacctctgcg
     2461 gtgcgcatca agttcgccaa gaaggttttg ccgactgcct cacgcagtgg ctggcgagcc
     2521 ctcaagcaag caatcgacac cactctggcg ggcacggacg aacccctggt gcttgtcatg
     2581 acggggcttg ccgcctctcg agaccgtttg agtctggagc gctttgctct caatgttcgc
     2641 gactacggta tgccggacaa tgaaggcgag ttggccgtgg acaccttcgt ggatcctgag
     2701 cagcccgacc tgcttcgcac catgttgccg cttccgccca tagttcgggc tgtggagcaa
     2761 gccggctatc cctgtcaggt gtccaaccat gccggcactt atatctgcaa cgagctttac
     2821 ttcaaggctc tttcttactg tcgccgcaac agccgggtga aagcagtagt cttcgtgcat
     2881 attccgcccc tggacaaatt tgccgctacg gctgaactct atggctccct tgccgtttcg
     2941 agacaggcat tcaatgccgt cgccaattct tctaattctc atcttcatca gcttgatctg
     3001 atgcggcatg ccctggagac gatcgtgaag gcggtg
//
LOCUS       DAOQNZ010000350         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16874_length_3036_cov_3.838980, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000350 DAOQNZ010000000
VERSION     DAOQNZ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16874_length_3036_cov_3.838980"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKW73_07415"
     CDS             <1..614
                     /locus_tag="PKW73_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73150.1"
                     /translation="NHLPTAFALAESAMLVEGGQVMLPDDASGLDCFYVDWRKNVSEI
                     LCAFLNIPDEQTQIVADFAPRPLSRAAWLVEVLKPDLLVLTAEGYQYDRLSGEHGEPA
                     SVMRELVGAIEKISPLNRDFQFGKNLQGGGQISGAPESTSVQEAQGKQLSDGLENATP
                     AARLSTRRYPMAVATEQELASALGLKFICGPEDELVRQQLGLN"
     gene            complement(754..1146)
                     /locus_tag="PKW73_07420"
     CDS             complement(754..1146)
                     /locus_tag="PKW73_07420"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNA73151.1"
                     /translation="MNMTTQCAPESLSLTIVCDERCTVVRSLAALLRVWDHGRRFTFV
                     DRESPSPIAAKLMSELDNSRWSLFLIDEQNEHWHGPDAIPMILKNLPFGKFAAVFYIL
                     PGTMWLTRNFYMVVSNLRFFLSGKRAAA"
     gene            1536..1931
                     /locus_tag="PKW73_07425"
     CDS             1536..1931
                     /locus_tag="PKW73_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73152.1"
                     /translation="MFVDEYTLLTQTIMPFLRDRWNEIDLVEGQSGERDGRLTKAELE
                     AARERFTAAGDEDAAAIIGELLMRYEAICAAFPDSAGEQEEESLGISREDVSVYAQLW
                     DPEYRKREGKPAPENWMEADSGPEKYWKP"
     gene            1928..2680
                     /locus_tag="PKW73_07430"
     CDS             1928..2680
                     /locus_tag="PKW73_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNA73153.1"
                     /translation="MKFEKKSLAVALGLIFLMIGGLLVYFLPQKMRPVLKLDATSRDL
                     GQVEEGKDLVASFLFSNEGFKPLEIFDVKTDCGCTDLSWKSVRIEPGKSSKLTVHIDT
                     AMKQGLVTKQIRFASNDPDMSFGSLNMQVEVENLHENLTVGGRAKIFSDEKCAHCHVD
                     MGVGAFGKELYDADCAMCHRPGKESKILVGPELEKGDFSDADYLKHVRQVISFGSKTH
                     KSMPGFLDAAGGPLAKEQVDSLVHYLKGLKKP"
     gene            2782..>3036
                     /locus_tag="PKW73_07435"
     CDS             2782..>3036
                     /locus_tag="PKW73_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73154.1"
                     /translation="MRKLGAIILLSAISSGFQPAFSRDFEVNPHAPNGYNASMAAYHY
                     HGGAPSEHNGYAASSIRSRHGMDSLMVPTSESGGGRFGGGG"
BASE COUNT          700 a          715 c          899 g          722 t
ORIGIN      
        1 ccaatcattt gccgacggct ttcgcccttg cagagagtgc catgttggtg gagggtgggc
       61 aagtgatgtt gcccgatgat gcctccgggc tggattgctt ttatgtagac tggcggaaaa
      121 atgtctcgga gattttgtgc gccttcctca acatcccgga tgaacagact cagattgtgg
      181 ctgactttgc gccgcgcccc ctgagccggg ccgcctggct ggtggaggtg ctcaagccag
      241 acttactggt tcttacagct gaaggctatc aatatgaccg cttgagtggc gaacatggcg
      301 aaccggctag cgtcatgcgg gagcttgtcg gtgccatcga gaagatttca ccgctcaaca
      361 gagattttca gtttggcaag aatttacaag gcggcggtca gatttccggt gctcctgaga
      421 gcacgagcgt gcaagaggcc cagggcaaac aattatcgga cggcttggaa aacgccactc
      481 cggcagccag gctttccaca cgtcgttatc ccatggccgt tgccacggag caagaattag
      541 cctcggctct ggggcttaag ttcatttgcg gtccggaaga cgaattagtt cggcaacagc
      601 tcggcttgaa ttagcagcgc ctgcttatag cggactgtat ctgaatgaac tggtgagaag
      661 agacttctac cggggaaact tgcaggaagc gctgagaaga ggcttctacc agggaaactt
      721 tctgcctttg gtttgccttc gaagcactgg ctttcaggcg gctgctcttt tgccgctcag
      781 gaagaaacgc aggttggaga cgaccatata aaaattgcga gtcagccaca tggtgccagg
      841 caaaatatag aagacggcag caaatttgcc gaagggcaga tttttgagaa tcatgggaat
      901 ggcatcggga ccgtgccagt gctcgttctg ctcgtctatg aggaagaggc tccagcgaga
      961 attatcgagc tcgctcatga gttttgctgc tatggggctc ggtgattcgc gatcgacaaa
     1021 agtgaaacga cggccgtggt cccagacccg gagaagggcc gccagagacc ttacgacggt
     1081 gcatctctcg tcgcatacga tagtgagtga cagggattcc ggggcacatt gtgttgtcat
     1141 gttcatacaa cgcccacctt acgataatag ttttttagag tgagtttagt ttagcagaag
     1201 ttcagagagt gtcacaccct atttggtggc aatttgaccg acttcttcag tttttaagtt
     1261 tgagcacttt ccagtttcaa cttgctcaag ggcaagtgat gaacaccgac gatgtcaagc
     1321 cgaaaatggt tccccggtcg gctcttgttt gatggtgtgt gcctgactgg ctgcctggga
     1381 ccggctaaac taaataggac cggctaaatc gggctggttg gcttgtaggc ggttgtgccg
     1441 agactccagg ttgtagaatg agtaaaggct tggaacaatt gtgtaagcaa gcttgcagca
     1501 cataaagcgg tcccctggag tagcaaattg gttctatgtt cgtagacgag tacacgcttc
     1561 taacgcaaac aattatgccc tttctgcgag atcgctggaa cgaaattgat ctggtggaag
     1621 gacagagcgg cgagcgtgat gggcggctga caaaagcgga gctggaagct gctcgggaga
     1681 ggttcaccgc tgccggcgac gaggacgccg ccgccatcat tggtgaattg ctcatgcgct
     1741 acgaagctat ctgtgcagcg ttcccggata gtgccggcga gcaagaggaa gagtccctgg
     1801 gcatctccag ggaagacgtc tcggtctatg cccaactctg ggaccctgaa taccgaaagc
     1861 gtgaagggaa gccggcgccg gagaactgga tggaagccga cagcggcccg gaaaaatatt
     1921 ggaagccatg aagtttgaga agaagtcttt agccgttgcg ctggggctga tcttccttat
     1981 gattggcggt cttctggttt atttcctgcc gcagaaaatg cgcccggtgc tgaagcttga
     2041 tgctaccagc cgcgaccttg gtcaggtaga ggaaggcaaa gacctggtgg ccagttttct
     2101 cttctcgaat gaaggcttca agcctctgga gattttcgat gttaagaccg attgtggctg
     2161 taccgatcta tcctggaagt cggtgcgcat tgaaccgggt aagtcttcca agttgaccgt
     2221 gcacatcgat actgccatga aacagggcct tgtcaccaag cagattcgct ttgcttccaa
     2281 tgatcccgat atgagtttcg gctcgctcaa catgcaggtt gaagtggaga acctgcatga
     2341 aaacttgact gtgggtggac gagccaagat ctttagcgat gaaaaatgtg ctcactgcca
     2401 tgtggatatg ggcgttggtg ccttcgggaa ggaactttac gatgccgact gcgccatgtg
     2461 tcatcgcccg ggtaaggaga gcaagatact ggtggggccg gagcttgaaa aaggcgattt
     2521 cagcgacgcc gattatctca agcatgttcg ccaggtgatt tctttcggta gcaaaaccca
     2581 taagtctatg cctggctttc tcgatgcagc gggcggacca ctggcaaagg agcaggtaga
     2641 ttcgctggtg cattatttga aggggctcaa gaagccttag ttttgccttg aatttggtag
     2701 tggcctgtgg ggctcgtgat tctcgatcga cccggcaggt ttatgatgta cataagcaac
     2761 tggtaaaggc gaaattaaag tatgagaaag ctcggtgcca taattctatt gagtgctatc
     2821 tcctcggggt tccagcctgc cttcagccgg gattttgaag tcaatcccca tgcgccgaat
     2881 ggatacaacg catcgatggc tgcctaccac tatcatggcg gcgcccccag tgagcataat
     2941 ggctatgctg caagctccat acgcagccga catggcatgg attctttgat ggtgccgacc
     3001 agtgaatccg ggggcggtcg cttcggcggt ggcggc
//
LOCUS       DAOQNZ010000351         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16880_length_3036_cov_2.662529, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000351 DAOQNZ010000000
VERSION     DAOQNZ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16880_length_3036_cov_2.662529"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1086
                     /locus_tag="PKW73_07440"
     CDS             85..1086
                     /locus_tag="PKW73_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73155.1"
                     /translation="MPDAGDTAFQNGDFSAAEVYFQEQLQTASQRDDTDRRNSLIQRL
                     ARTLICQRKYAEAEQLFESFPQPASLVHAIHVSILGETVGAGNFQKACTLFKNVIGEE
                     PVTNTLNMLNARAANHEALTVKCELYTELFNWNWFDPMAGQFFQHFATILANRLRKEE
                     HYQTAAEIFEELSLLLPGNPFALACLLEVQMEAGDYSKAMSTLNNLKHRLKANNEADA
                     TFFEIVGLCYEAQLSAVQGDERLANKLFVNAINMAKIPSFNAWLIQDWSNEIEAMAAG
                     KEGKPWSTKAPESFNYAAALHCLISYRNFHNNYGISSYSRQIEQVAEHIKQFIPSSS"
     gene            complement(1158..2270)
                     /locus_tag="PKW73_07445"
     CDS             complement(1158..2270)
                     /locus_tag="PKW73_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73156.1"
                     /translation="MKFQRFGVLPICASLLFLVAARAEASPQSHSSWPKLDSKYSLPE
                     CNQVLNAARLLFNSTAPEFSEDCQPVGKAKLGVILGPLPNYRDCESDCAFWFDSTYIE
                     ADNPAGERHRIFKQKDAESGSRFVVGQKPMNWQGDWFDLYCVDANMPVNKVEALVNES
                     GDSGQVENKKMAGAYIVYRDLWHRPWLFRNPTNKHVVAVNPRSDWLQRNEKVIPDWAI
                     YRASASGQAELIGIIRFSAEAKPLALLPNGPLHEMFALLDNIIGIPSQSEGTLQPTAR
                     LRGETRVAVGNLMLRPWALGQPKNSKEEVEKGLQRWSKGSVVYRKQYEQLKLLYPRAL
                     DALTVYYQKTFKEPPAKARALAVRSLDLAYRSHFAF"
     gene            2630..>3036
                     /locus_tag="PKW73_07450"
     CDS             2630..>3036
                     /locus_tag="PKW73_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNA73157.1"
                     /translation="MTLFDRYVVRETLQVLLIGTLAIIGIFFGTAEFTNVLNLMNETG
                     LPLHTVFSVMLLQLPTGLIFCMPAGVVVAVMLVLQRQNRDSEVVALQLQGIPLRRVLM
                     PFLALGLISTSIAFAVSENLAPQSRDLSRRLVTL"
BASE COUNT          819 a          810 c          697 g          710 t
ORIGIN      
        1 aacagagtaa gtggaataac tagtgagtct ctctaactag aagccgcttc cgccggagaa
       61 aacacagtgc ccgagacaaa caatatgcca gatgctggcg acacggcttt tcaaaacggt
      121 gacttttcag ccgccgaagt ctactttcaa gaacaattgc aaacggcaag ccagagggat
      181 gacacagacc ggagaaatag cctcatccag cgcctggctc gcactttaat ctgccagcgc
      241 aaatacgcag aagcggagca gctttttgag tcgtttccac agccggcgtc ccttgttcat
      301 gccattcacg tttccatatt gggcgaaact gtcggtgccg ggaattttca gaaagcctgt
      361 acgctcttta agaacgtaat cggggaagag ccagtaacaa acacgttgaa catgctcaac
      421 gccagggcgg ccaaccacga agctctcaca gtcaaatgcg agctctatac agagctattc
      481 aattggaatt ggtttgatcc gatggccgga caattcttcc aacatttcgc gactatcctg
      541 gctaacaggc tacgtaaaga agagcattac caaaccgctg ccgaaatttt cgaggaactg
      601 agcctgcttc ttccaggaaa tccatttgct ctagcctgct tgttagaagt acagatggag
      661 gcaggcgatt actcaaaagc aatgagtact ctaaacaatc taaaacacag actgaaagcg
      721 aataacgaag ccgacgccac cttctttgaa attgtagggc tttgctacga agcccaacta
      781 tcggcagtgc aaggcgatga gaggcttgcc aacaaactat ttgtgaatgc aataaacatg
      841 gcgaagatac ctagcttcaa tgcctggctt attcaagact ggagtaacga aattgaggcc
      901 atggcagcag gcaaagaagg aaaaccatgg agcactaaag ctccggaatc attcaactac
      961 gctgccgctt tgcactgcct cataagctac agaaactttc acaacaacta cggaatatca
     1021 tcatactcac gccaaatcga acaggtggct gagcacatca agcagtttat acctagctct
     1081 agttaaattc caggtagcgc ctgtgcagaa caatcgagaa cctatctaat gcagctgacc
     1141 aaattcgagg cagttgctca aaaggcaaaa tgactgcggt aagccaaatc gagtgatctg
     1201 acggcaaggg cacgggcttt agcaggcggc tctttgaacg ttttctggta gtagacagta
     1261 agcgcatcaa gggccctggg gtaaagaagc ttcaactgct catactgctt cctgtaaaca
     1321 acacttccct tactccatct ctgcaagccc ttctcgactt cttctttgct gtttttaggc
     1381 tgccctaagg cccacggtct tagcatgaga ttgccaactg ccacccgcgt ctcgcctctc
     1441 aatctggctg tcggctgcag agtaccttca ctctggctgg gaatgccgat gatattgtcg
     1501 agtaaggcaa acatttcgtg caggggtcca ttaggtaaaa gtgcaagcgg cttagcttca
     1561 gccgagaacc tgatgatacc gataagctca gcctgaccgg aagcagaagc tctgtaaata
     1621 gcccaatcgg gtatgacttt ctcattccgt tgcagccaat cagagcgagg attaacagcc
     1681 accacatgct tattggtagg attgcgaaat aaccacgggc ggtgccacaa atctcgatag
     1741 acaatgtaag caccggccat cttcttattc tctacctggc cgctatcacc tgattcattc
     1801 accagggctt ctactttgtt gaccggcata ttcgcgtcta cacaatagag gtcaaaccaa
     1861 tcgccctgcc agttcatggg tttctgcccc accacgaaac gacttccgct ctctgcgtct
     1921 ttctgcttga atatgcggtg acgctcaccg gccggattat cagcttcaat ataggtagaa
     1981 tcgaaccaga aggcacagtc actctcgcag tcacggtagt taggaagagg accaagaatg
     2041 acaccgagct tggctttccc tacaggctgg cagtcctcac tgaactccgg ggcagtgctg
     2101 ttaaagagca gcctggcagc attcagcacc tgattgcatt ccggcaaaga atatttagaa
     2161 tcaagcttgg gccaggatga atgagactgg gggctggctt ctgccctggc agccactaga
     2221 aaaagcaaac ttgcacaaat cggcagaact ccaaatctct gaaatttcat aagcctccaa
     2281 caacattctg gcgacagaac catacacatg aggggaagtc ccctaccggt agcccaaggc
     2341 ctgggcacag acacaggatc gccttcttcc ttaactattg gctactggaa agcatccggc
     2401 agataagcta ggctagagcg gttccaatga ctatctccat ggcgacctcc actatctttg
     2461 acatcatttt gctttcagac accacggcaa agcaccattt ttcacactgg tgccggatgc
     2521 acctccattg gcggcaccca agcatggaga gagaagagcg gaaacgtcaa atccccgggc
     2581 gcgcacaact atctaccgcc caggtcattt ttttagtcgg gtgcttgcaa tgactttgtt
     2641 cgaccgttat gtcgtcagag aaaccttgca ggtacttttg atcggtaccc ttgccataat
     2701 cggtatcttt tttggcactg ccgaatttac caatgttctc aatttgatga acgagaccgg
     2761 tttgcccctg catacagtct tctccgttat gctcttacaa ctgcctaccg gccttatctt
     2821 ctgtatgccg gccggagtag tggtggcagt catgctcgta cttcagcgcc agaacagaga
     2881 ctctgaagtg gtggcattac agcttcaagg cattccactc agaagagtac tgatgccatt
     2941 tctcgccctt ggcctcattt ctacaagcat tgcctttgcc gtaagcgaaa atctcgcacc
     3001 acaatcacga gacctatcac ggcggctcgt caccct
//
LOCUS       DAOQNZ010000352         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_16980_length_3027_cov_4.028600, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000352 DAOQNZ010000000
VERSION     DAOQNZ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16980_length_3027_cov_4.028600"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKW73_07455"
     CDS             <1..493
                     /locus_tag="PKW73_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73158.1"
                     /translation="LVLASAYLGLAKYCWTPLFLANDKRLFYSVEGFFLSIALLAILI
                     GLRPFLTPSSLQLSHYGIKYRGPYWPQRKSVNWSQIRKVYLSPELIIVLYHPQPGKKR
                     LWPLIIPSIYLADRDRISQVFTRYSPIKAINLSSPAPISRIVMVLLFLGAVIWLLEML
                     ITQ"
     gene            complement(516..824)
                     /locus_tag="PKW73_07460"
     CDS             complement(516..824)
                     /locus_tag="PKW73_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNA73159.1"
                     /translation="MSEEMKRIDQVKKAQRERAMEGSTNLTGEQIFIRSCNTCHPSAK
                     AGMGPSLEAMNAKFPEDQALKQFIRKGKGMMPGQSVERINNDELDNLITYLRALEFSK
                     "
     gene            1351..2661
                     /gene="murA"
                     /locus_tag="PKW73_07465"
     CDS             1351..2661
                     /gene="murA"
                     /locus_tag="PKW73_07465"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNA73160.1"
                     /translation="MTQVTRLDPPAKQGPSEAEERIIIRGGKPLHGQVRVFGAKNAVL
                     KMMAAALLAKGVTVLRNVPNLTDVHMMAEVLRHLGAKVEVGNDEVKIDATDLTEIEAP
                     YELVSQLRASFVVLGPLLARCKEAKVSLPGGCAIGERRIDLHERGLKLLGAEVGIDHG
                     YVYASAKKLVGTRVYLDRPSNGATENIMLAAVLAEGQTIIENAAQDPEIVNLADCVNA
                     MGGKITGAGTYQIVIDGVPLEEMHGAVVDTIPDRLEAGTFLYACMAAGGEVIIDGVNA
                     HHIYAVISKIHEMGAECSIYAPDTIKIKSTGRLKPTDITTVPYPGFPTDLQAPLMAVL
                     AVAEGTSIVTETIYENRFMQVGELRRMGADIQLKANSAIVKGVPKLMGAEVKSSDLRA
                     AASLIIAGLGADGVTEVTGLSHLDRGYDRLEERLRALGADIWRR"
     gene            2740..>3027
                     /locus_tag="PKW73_07470"
     CDS             2740..>3027
                     /locus_tag="PKW73_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73161.1"
                     /translation="MIVMKFGGTSVGSKERFEQVYGLIERARQRSAPPLVVVSAMSGV
                     TNALIESANLAVRRDLEGALNQIEMVRNKHIDAVKGLLSKEKADLLLADLNG"
BASE COUNT          740 a          893 c          721 g          673 t
ORIGIN      
        1 cctggtgctg gcctcggctt atcttggtct ggccaagtac tgctggacac cgctctttct
       61 cgccaacgac aaacggctct tctacagcgt cgaaggtttc tttctctcca tcgcgctctt
      121 agccatcctg atcggactga ggcccttcct gacaccatcc agcctgcagc taagccatta
      181 cggtatcaaa taccgtggtc cttactggcc tcagcgcaaa tccgtcaact ggagccagat
      241 tcgcaaggtc tacctgagcc ccgagttgat catcgtgctc taccaccccc agccgggcaa
      301 gaaacggctc tggcccttga ttatcccctc tatctacctg gccgaccgcg accgcatctc
      361 ccaggtcttc acccgctatt cgcccatcaa agccatcaac ctgtcgagcc cggcgcccat
      421 ctccagaatc gtcatggtgc tcctcttcct gggagccgtt atctggcttt tggaaatgct
      481 tataacccag tagcgatgcc gcttgttcag gccaattact tgctgaactc gagagcgcgc
      541 agataagtaa tcagattatc aagctcgtcg ttattgatac gctcaacgct ctggccgggc
      601 atcataccct tgcctttgcg aataaactgc ttgagggcct ggtcttccgg aaacttggcg
      661 ttcatcgctt ccagactagg ccccatgccg gctttggctg agggatggca ggtattgcaa
      721 ctgcgtataa aaatctgctc gcccgtcaaa ttggtggagc cttccatagc ccgctcccgc
      781 tgagccttct tcacttgatc gatgcgcttc atctcttccg acataacaca tccggacaat
      841 gcggaagagc cggtaaacat tgcaagtagc gccaggaaga acaggccggc tgtaggccgg
      901 ggtgagcgcc cggcgccccc cttctgcgaa gcgaccgagc aggccgcatc agggcgaaac
      961 ctattagaca tattgcaaaa tcctcacccg aaaacctctt gacagccaga gtttaccgca
     1021 cttttacatc ctactttata actgttttca atatctcaaa tcaaaagtag gtcaggaagc
     1081 cgggcctatc ttaggacgtc aaaaccctaa tcaaaaatat gcttaacatt gagtttacct
     1141 ttacgttgta gagtaagtac agaggcgaaa tgtagtttct ttcaaggcaa acgacaggca
     1201 gcagcaatac agatcacctg gctatccagc caatatcctt cgtgattttt tcggtatcaa
     1261 aacaaaatct gctcctaatc catttcttct ttcagtaagc gataaaacaa gcttctcagt
     1321 acaacttcct aatagaaaag tggcgacaat atgacacaag taaccagact cgatcccccc
     1381 gccaagcaag gccccagcga agcagaagaa cgcataatta ttcgtggcgg caaaccgctg
     1441 catggtcagg ttagagtttt cggtgctaaa aatgccgtcc tcaaaatgat ggctgccgcc
     1501 cttcttgcca agggagtaac ggttctgaga aatgtgccca atctcaccga tgtgcacatg
     1561 atggctgaag tgctcagaca ccttggcgcc aaagtagaag tcggcaacga tgaagtcaaa
     1621 atcgacgcca ccgatcttac cgaaatcgaa gccccctacg aactggtcag ccagttgcgc
     1681 gcctccttcg tagttctcgg gcccctcctg gctcgctgca aagaagccaa agtttccctc
     1741 cccggcggct gcgccatcgg cgagcgccgc atcgacctgc acgagcgcgg tctcaaactt
     1801 ctcggtgccg aagtgggcat cgaccacggt tatgtttatg cctccgcaaa aaaactagta
     1861 ggcacacgtg tttacctcga tcgcccctcc aacggtgcca ccgaaaacat tatgctcgct
     1921 gctgttctgg ctgaaggtca gaccatcatc gaaaacgccg ctcaagatcc tgaaatcgtc
     1981 aacctggcag actgcgtcaa cgccatgggc ggcaaaatca caggcgccgg cacctaccag
     2041 atcgtcatcg acggcgtacc gctggaagaa atgcatggtg ccgtagttga taccattccc
     2101 gacagactgg aagccggtac tttcctctat gcctgtatgg ctgccggtgg cgaagtaata
     2161 atcgacggcg tgaacgctca tcacatctac gctgtaatct ctaagattca cgaaatgggc
     2221 gctgaatgtt ctatttacgc ccccgacacc atcaagatca agagcaccgg cagactcaag
     2281 cctaccgaca tcacaaccgt gccttatcca ggcttcccca ccgacctgca agcgcctctc
     2341 atggctgttc ttgccgtcgc tgaaggtaca tcaatcgtta ccgaaacaat ctacgaaaat
     2401 cgcttcatgc aagttggtga attgcgtaga atgggagccg acatccagct caaagccaat
     2461 tcggccatag tcaaaggtgt gcccaaactg atgggcgccg aggtcaaatc aagcgacctg
     2521 cgcgccgccg cctctttgat catcgccggt cttggtgccg acggcgttac cgaagtaacc
     2581 ggtctcagcc acctcgacag aggttatgac cgcctcgaag agcgtctgcg ggctctgggt
     2641 gccgatatct ggagacgcta gaaatcttcc ggcatctgct tcagatttct gaccgttgac
     2701 cgtaaagtaa gtcagttctc aacgctcaag taatcgacaa tgatcgtaat gaaattcggt
     2761 ggcacttccg ttggctcgaa agagcgcttc gagcaagtgt acggactaat cgaaagagcc
     2821 cgccaaaggt cggcaccgcc cctggtggta gtgtcggcca tgtccggcgt caccaatgcc
     2881 ctgatcgaat cggccaacct ggccgtcaga cgagatctgg aaggcgccct caatcagatt
     2941 gagatggtgc gcaataagca tatcgacgcc gtcaagggtc tcttgagcaa agagaaagca
     3001 gatctgctct tagccgacct gaacgga
//
LOCUS       DAOQNZ010000353         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17011_length_3024_cov_3.843045, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000353 DAOQNZ010000000
VERSION     DAOQNZ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17011_length_3024_cov_3.843045"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="PKW73_07475"
     CDS             <1..1447
                     /locus_tag="PKW73_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA73162.1"
                     /translation="RADELAQLISEEVGKPLAEAYLSELTGPLDTCVWMVENAEKLLA
                     DQSISLSNPLLSSKQSLVAFEPLGVVGIIAPWNYPFSIPMMATIMSIMVGNTVIIKPS
                     EKSPLIGIKIGEICQEAGLPHGVVEIVTGDRETGKYLSEAALNKLIFTGSVQGGQKVM
                     AQCADNLTPLTLELGGKDAAIVLPDAPIDWTCRGITWGAFTNAGQACASVERVYLIKG
                     KNTEKIISKLTKLASGLKLGPGTSDSTDVGPLVDEVQLAKVKDQVEDALSKGATILTG
                     GKAREDLGGFFFEPTVLTNVDHTMKVMTEETFGPLLPIMVVETEDKAVELANDSQFGL
                     CATIWAGKLERAECVARDLNVGTALINDCLFSHATPQLPWGGIKKSGFGRSHSYFGLY
                     DLVNIKHISIDSAGGSHRVWWYPYGKAKVKMARGGIQMLHGKMTERPVGLVHFVGNMF
                     VGPQSENPTGKAAIKPESEAAKTDANPDKPQ"
     gene            complement(1467..2654)
                     /locus_tag="PKW73_07480"
     CDS             complement(1467..2654)
                     /locus_tag="PKW73_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNA73163.1"
                     /translation="MEALIYLDNRATTRVRPEVLEAMLPYLSENFGNPSSIHTQGRKA
                     REAVEQARQEVADLLGCQPREVYFSSCGTLSNNVALLGRARFVEANGLNKHLVVSRIE
                     HPSVMGPAQYLESQGFRVTYLPVDREGVIRLEALETALRQGASMVSIMWANNEIGVLQ
                     PVEAITELVEKVAAEQGREIYQHCDAVQVIGKLPVDLEKLKLSSLSFSGHKFGAPKGI
                     GALFLRRLVNVMPIIFGGGQEMGLMPGTEPLANIVALGVASRLARLEQEELQKRLLGY
                     QEKLIGALLQLPGVELTGPSSAHRRLPGHVSFCAGGIEGEALVLKSDLRGMAISSGSA
                     CHQGIIEPSAVLRAIGLSPEKALGSVRITFGRDSSDADVEKACAILQKLLTGVLPGAE
                     RGG"
     gene            2752..>3024
                     /locus_tag="PKW73_07485"
     CDS             2752..>3024
                     /locus_tag="PKW73_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNA73164.1"
                     /translation="MYLRLTILLLLLLAAFTANLCLGEVNLSPLQAWPLLYGQGDEAN
                     LQVIREIRLPRLLSAALIGLALSTAGYLLQKLSRNQLADPYLTGVSS"
BASE COUNT          778 a          840 c          774 g          632 t
ORIGIN      
        1 ccgtgccgat gagctggcgc aattgatcag cgaagaagtg ggtaagcccc tggccgaagc
       61 ctatctttca gaactcaccg gtcccctcga tacctgtgtg tggatggtgg agaatgcgga
      121 aaaacttctg gcggatcaaa gtatctccct ttccaatccc ttgctttcca gcaagcaaag
      181 cctggtggcc ttcgagcccc tgggagtagt ggggataatc gccccatgga attatccctt
      241 ttcgattccc atgatggcca ccataatgtc cataatggtg ggcaatacgg tcatcatcaa
      301 gccctccgaa aagtcgcccc tgattggcat caagattgga gaaatctgcc aggaagccgg
      361 tttacctcac ggtgttgtgg aaatagttac gggcgacaga gaaacaggca agtacctgag
      421 cgaagccgct ctgaacaaat tgatatttac aggctccgtg cagggcggtc agaaagtgat
      481 ggctcaatgc gccgacaatc tcactccgct caccctggag ctgggcggta aagacgctgc
      541 catagtactg cctgatgccc ccatagattg gacctgtcgt ggtatcacct ggggtgcctt
      601 caccaatgcc ggtcaggcct gcgcatcggt cgaaagagtc tacttaatca agggcaagaa
      661 tacagaaaaa atcatctcaa aactaacaaa attggcttcc ggactaaagc ttggaccggg
      721 gaccagtgac agcaccgatg tcggtcctct ggtggacgaa gtgcaattag ccaaggtgaa
      781 agaccaggtg gaagacgcgc tcagcaaagg tgccaccata ctgaccggtg gaaaagccag
      841 agaggatctg ggcggcttct tctttgaacc tactgttttg acaaacgtag atcacaccat
      901 gaaggtcatg accgaagaaa ccttcggacc gctcttgccg ataatggttg tcgaaacgga
      961 agacaaagct gtggaactgg ctaacgatag ccaatttggc ctctgcgcca caatctgggc
     1021 cgggaaactg gaacgtgcag aatgcgtggc aagagatctt aacgtcggca cggccctcat
     1081 aaacgactgc cttttcagtc acgccacacc acagctcccc tggggcggca taaagaaaag
     1141 cggcttcgga agaagtcatt cttactttgg cttgtacgac ctggtcaata tcaaacacat
     1201 cagcattgat tcagccggcg gatcgcaccg agtatggtgg tacccatacg gcaaagcgaa
     1261 agtaaaaatg gccagaggcg gcatccagat gctgcacgga aaaatgaccg agagaccggt
     1321 tggcctggta cattttgtcg gcaatatgtt tgtcggtccg caatcagaaa accccaccgg
     1381 caaggccgca atcaagccgg agagcgaagc agccaaaaca gacgccaacc ctgacaaacc
     1441 tcaataacag agccgaaaag aaaagtttag ccgccgcgtt cagcccctgg cagcaccccg
     1501 gtcaataact tctgcaagat tgcgcaggcc ttttccacat cggcgtcgct tgaatctcga
     1561 ccaaaggtaa tgcgcaccga acctaaagct ttctcaggtg aaagaccgat ggccctcaat
     1621 accgccgaag gttcgatgat gccctggtgg caggcactgc cactggatat ggccatgccc
     1681 ctcagatcac tcttcaagac aagcgcttca ccttcgatac cgccggcgca aaaactgaca
     1741 tggccgggaa gacgccggtg cgcggaactt ggtccggtca gttctacgcc gggcaactgc
     1801 aagagagccc caatcaactt ttcctgatag ccgagcaggc gcttctgtag ctcctcttgc
     1861 tcaaggcgag ccaggcggga ggcaacaccc agagccacaa tgttagccag aggttcggta
     1921 ccgggcatca agcccatctc ctgtccaccg ccaaagatga taggcatgac attcacgagg
     1981 cgccgcaaga ataaggcacc gattcctttg ggagcaccga atttatgccc cgagaaagag
     2041 agactggaga gtttcaactt ctcaaggtca acaggcagtt tgccgattac ttgcacggcg
     2101 tcgcaatgct ggtaaatttc acgcccctgc tccgcggcta ctttctcgac cagctcagta
     2161 atggcctcga caggctgcaa tacgccaatt tcattgttgg cccacataat cgaaaccatg
     2221 gaagccccct gcctgagagc agtctccagc gcctccaggc ggataacccc ctctctgtcc
     2281 acaggcagat aagtgacgcg aaagccctga ctctccagat actgtgccgg tcccattaca
     2341 gaaggatgct ctatgcgaga aaccacaagg tgtttgttta agccgttagc ttctacgaag
     2401 cgagcccgcc ccaggagggc tacattgttg gacagtgttc cacagctgga aaaataaact
     2461 tcccggggtt ggcaaccgag taaatcggca acttcctgcc tggcctgttc gacggcttcc
     2521 ctcgccttgc gcccctgagt gtgaatcgaa ctgggattgc cgaaattttc cgaaagatag
     2581 ggcagcatgg cctccagaac ttccggacgc acacgggttg ttgctctgtt atcgagataa
     2641 atcaaggctt ccatcaaccg gtaccattta gtaagacggc tggcaacatg gtaacatccc
     2701 ctcacaaagc aatttcttat ttgtaatgta agtgattcaa gaattagtta agtgtatctg
     2761 cgactgacaa tcttactact cttgctcctg gcagcattca cagccaatct ctgcctgggt
     2821 gaagtaaact tgagcccgct ccaggcctgg cccttacttt acgggcaagg agatgaggcc
     2881 aacctacaag tgatacgaga gattcggctg ccgcggctac tgagcgcagc gctgattggc
     2941 ctggctcttt ccacggccgg ctacttactg caaaaactct cccgcaacca actggcagat
     3001 ccttatttga ccggtgtttc cagt
//
LOCUS       DAOQNZ010000354         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17012_length_3024_cov_3.718761, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000354 DAOQNZ010000000
VERSION     DAOQNZ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17012_length_3024_cov_3.718761"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKW73_07490"
     CDS             complement(<1..375)
                     /locus_tag="PKW73_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73165.1"
                     /translation="MSKNEKEIEQKKYAYRWFIALVCVLVLSIILLLAIQYHWGGWFP
                     DRKPESYDPIDPLTWVLAALIGAALYLMGQIATYYPKIKEKLDPNYQNDFVGSTYWYA
                     STLIRAPVLTVVILWLLTNLSVD"
     gene            complement(410..925)
                     /locus_tag="PKW73_07495"
     CDS             complement(410..925)
                     /locus_tag="PKW73_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73166.1"
                     /translation="MPTKPKDRSGLLWLALLPILSILIDHYLNSAAVWRYAAVCLPWL
                     VTGAVFKSVAREDLPEDAKSAAGRLLAGMALLMVLWYLPAWLPFLRSSDYGMALNLRE
                     SPVVVWMGAAFVLGFYRKLITKSFAVLGNGIKNYFIQKTGLELPEDETLKPAEVWKGL
                     KSLFKDLTRRS"
     gene            1169..1798
                     /locus_tag="PKW73_07500"
     CDS             1169..1798
                     /locus_tag="PKW73_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNA73167.1"
                     /translation="MPKPVPPRHFHVSDLETLRAISDPLRIQILELLEGAPLTVKQVA
                     GKLGLAPSKLYYHFGALEKLNLIEVAETRMVANMVEKTYINSASTLTVDPALFQFSKE
                     GDNESINILLASTIDATREDILRSLQARQFQLHQGSAEHERRMIINRVVSRVPESRIA
                     EFQQRLVQLVQEFGAEEKTAKASDQLYALSVAFYPSFYFDSKPKKKRSK"
     gene            1795..2727
                     /locus_tag="PKW73_07505"
     CDS             1795..2727
                     /locus_tag="PKW73_07505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNA73168.1"
                     /translation="MSAFVVGLYLVLYLAALALAFRSRKTFPLSESIMVMLILGLGFT
                     SLVYVVAPRIADFTPAPVPASELAFVLAYVGLVSVLLVVVNPVPPALRADFLKSKLIS
                     LAYKLTLFVIIPLTSLRLFWNVSWEALGFTVQDLPGQWVAALILSLCFGGFNLVAGSG
                     AKPLRAGAFTPAQVLLGFPAVFFWNVLEVGLVEEFFFRAFLQGTLMQALGSSLAGICA
                     ASLLFGLAHAPGLYLRRADKDGPLGERPTLLNAILYAVLVLSPAGWFTGLLYLRTGSL
                     LVPVLVHAAIDTVAHVSAAITAFLPASRAGAQSA"
BASE COUNT          583 a          988 c          725 g          728 t
ORIGIN      
        1 gtcaaccgat aggttggtca gcagccatag gatcaccacc gttagcaccg gcgctctgat
       61 cagggtgctt gcataccaat aggtgctgcc gacaaaatcg ttctggtagt ttgggtccag
      121 cttttctttt atcttcgggt aatacgtcgc gatctgcccc atcaggtaca gcgccgctcc
      181 gatcagcgct gccagtaccc aggtcaacgg gtcgatcggg tcatacgatt ccggctttcg
      241 atccgggaac cagcctcccc agtgatattg gatcgccagc aacaagatga tacttagcac
      301 caatacgcag acgagggcaa tgaaccaccg atatgcatat ttcttttgct ctatttcttt
      361 ttcgttcttg ctcatgattc tctccttttg tctctgagag gcggcccaat taggaacgtc
      421 tcgtcagatc cttgaacagg cttttcaatc ccttccatac ctcagcgggt ttgagggttt
      481 catcctcggg caattccagc ccggtcttct ggataaagta attcttgatt ccgtttccca
      541 gcactgcaaa agatttggtg atcagtttcc tgtaaaaccc caatacaaaa gccgctccca
      601 tccagaccac aaccgggctt tcccgtaggt tgagagccat cccgtaatcg ctcgatctta
      661 aaaaaggcag ccacgcgggc aggtaccaca acaccatcag cagggccatc ccggccagca
      721 ggcgtcctgc ggcgctcttc gcatcttccg gcaggtcttc cctggccacc gacttgaaga
      781 ccgcccccgt taccagccag ggcaggcaca cagccgcata ccgccatacg gctgcgctgt
      841 tcaggtaatg gtcgatcaaa atggagagga tggggagcag ggccagccac aacaggccgg
      901 accggtcttt tggttttgtt ggcatctcat tccttctggc tcgttcaaag caacccggag
      961 aacgctcaac agcaccgtta aagcagggga gttatgctta ccgattaaac cctactattc
     1021 atcatagtga atttatgtac gttgtcaact ttgtctgtat tttcacaaac cctcttggag
     1081 cacttcctcc cggcccttga ctctttcctg attccttata aaatgtacct tatcgttaaa
     1141 aataatttta gcgataggag aaccttttat gcccaagccc gttccgcctc gtcacttcca
     1201 cgtttccgac ctcgagaccc tgcgcgccat ctccgacccc ctgcgcatcc agatcctcga
     1261 gctgctcgaa ggcgcgcccc tcaccgtcaa acaggtcgcc gggaagctcg gcctggcccc
     1321 cagcaaactg tactatcact tcggcgcgtt ggagaaactc aacctcattg aagtcgccga
     1381 gacgcgcatg gtcgccaaca tggtcgagaa gacctacatc aactctgctt ctaccctcac
     1441 ggtcgacccg gcccttttcc agttctcaaa agaaggcgac aacgaatcca tcaacatcct
     1501 gctcgcctcc accatcgacg ccacccgtga agatatcctc cgcagcctgc aggcgcgcca
     1561 gttccagttg catcagggca gcgccgagca cgaacgccgt atgatcatca accgtgtcgt
     1621 cagccgtgtg ccggagagcc gcatcgctga gtttcaacaa cgcctcgtcc aacttgtgca
     1681 agagttcgga gccgaagaaa agaccgccaa agccagcgac cagctctacg ccctgtcggt
     1741 ggccttctat cccagtttct actttgattc aaaaccgaag aagaagaggt ccaaatgagc
     1801 gcgttcgtgg tcggtctcta cctggtcctg tatcttgccg cgctggctct ggccttccgg
     1861 agccgcaaga ccttccccct ctctgagtcc atcatggtca tgctgatcct cggcctgggc
     1921 ttcacctctc tggtctatgt ggtcgccccg cggattgcag acttcacccc cgcgccggtc
     1981 ccggcctctg aactggcctt cgttcttgct tatgtcggcc tggtctcggt cctgttggtt
     2041 gtcgtcaacc cggtaccgcc tgccttgcgc gcagacttcc tcaagagcaa gctcatcagc
     2101 ctcgcctaca aactgactct ctttgtcatt atccccctga cgtccctgcg cctcttctgg
     2161 aacgtcagtt gggaagcgct tggtttcacc gtgcaagacc ttccgggtca gtgggtcgcg
     2221 gccctcatcc tctccctgtg cttcggcgga ttcaatctcg tagcgggcag cggtgccaaa
     2281 cccttgcgag ccggtgcttt caccccggct caggtactgc tcggctttcc ggccgtcttc
     2341 ttctggaatg tgctcgaggt cggtttggtg gaggagttct tcttccgcgc ctttcttcag
     2401 ggcactctga tgcaagccct cggcagttcg ctggcgggta tctgcgccgc ctccctcctg
     2461 tttggtttgg ctcatgctcc cggcctctac ctccgccgcg ccgacaagga cggtccgctc
     2521 ggtgaacgtc ccaccctgtt aaatgccatt ctctatgccg tgctcgtgct cagcccggcg
     2581 ggctggttca ctggtttgct ctacctccgc accggaagcc tgctcgtgcc tgtccttgtc
     2641 catgccgcca tcgataccgt cgcgcatgtc agcgctgcca tcaccgcctt cctgcctgcc
     2701 tcccgcgccg gagcccagtc tgcataatcc ataccctccc tatgtcattg cgagggttga
     2761 cggtcgagta gagcggtgtt cttccgctca tatcgagacc ctccccgaag cgatccttta
     2821 tttatccttg taatccttaa gtgccttacc catagtagga gaacccatga ccaccctcga
     2881 aacgaccccg cttgaaacac cgcgcgacat gaagaccttc ttcaccatct ggagcgggca
     2941 gttggtctcg atgctcggct ccggtctgac cagcttcgcc atcggcgtct ggatctacga
     3001 ccagagcaaa caagccacgc cctt
//
LOCUS       DAOQNZ010000355         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17059_length_3020_cov_3.681956, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000355 DAOQNZ010000000
VERSION     DAOQNZ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17059_length_3020_cov_3.681956"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKW73_07510"
     CDS             complement(<1..366)
                     /locus_tag="PKW73_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73169.1"
                     /translation="MKNKAIAGSRAERTRTFPGLPLVALAFTVIANQSVSAAPQWQAM
                     NSSPGTIGAIKTEQKETGLTTLRQAIKDSQVSLSVQGDGYTTSSVRLLLTNKTKLPLK
                     LVIPANEVLHPNTSTVQTMM"
     gene            718..1473
                     /locus_tag="PKW73_07515"
     CDS             718..1473
                     /locus_tag="PKW73_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73170.1"
                     /translation="MKFLASLMGFLLAFQMTCLPLPADAAVDYKQSSGMTGQVEVNKV
                     GALLLAGGGLATLAGTAIEISTKGSTSSPTLKGQLFNMSQSGSSISGHAFFDEGSGLS
                     QLSPDGCSEFVKLIDGSKVNGPISSVTLDAVTCAGQSIPCSRIASISSARVFKFTTKI
                     GESPRMTFQPSCVKLAGASKTYEAPTWKKIVIVGACVTALACAITLPIVIPLATRGNN
                     HHNDLQNAYIYNTYLNASRVQPPPQPVRSGSGP"
     gene            complement(1541..>3020)
                     /locus_tag="PKW73_07520"
     CDS             complement(1541..>3020)
                     /locus_tag="PKW73_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA73171.1"
                     /translation="VTGATGYVGARLIPLLLEAGYLVRAAGRSLAKLKARPFAGQEGV
                     ELCSLNVLERADLKAALKDVYAVYYLVHSMNSKSRDFAEADRLAAKNMAAEAEEAGVR
                     RIIYLGGLGEDSPELSKHLRSRAETGEILKSGKVPVTILRAGMILGSGSTSFEILRYL
                     VERLPFMITPRWVKTPSQPIAIRNVLNYLVGCLGIEATTGETYDIGGPEVITYMELMQ
                     TYARRAGLPRRVILPVPVFTPRISSYWIHFVTPVPAYIARPLAEGLRNPVVCKNNEIL
                     KLIPQDLLDADTAIGIALERMRSQTVESSWTDAGSMPADEWLAPGDPGWAGGTYYEDS
                     RIITVSDTTKEVWQRLVRLGGKTGWYYGNWLWKLRGFMDRMLGGVGLNRGRRNENELG
                     VGDALDFWRVVEVEEEKHLLLLAEMKLPGEAILEFQICPQGTHTQVRQIARFVPHGLL
                     GLLYWWAVTPLHEFVFSGMLKGVAAKSPATIVEGPHKQPGKS"
BASE COUNT          616 a          816 c          818 g          770 t
ORIGIN      
        1 catcatggtt tgcactgtgg atgtattggg atgcaatact tcgttggcag gaatgaccag
       61 cttgagcggc agcttggttt tatttgtcaa aagcagtcgc actgacgatg ttgtatatcc
      121 gtcgccctgc acgctcagtg aaacctgact atctttaatg gcttgccgta aggtggtcag
      181 cccggtttcc ttctgctcgg ttttgatggc gccgatggtg ccgggcgaag aattcatcgc
      241 ctgccattga ggcgccgcgc ttaccgattg gttggctata acggtgaatg ccagggctac
      301 taagggtaaa ccgggaaagg ttcgggtgcg ttcggccctt gagcccgcta tcgctttatt
      361 cttcatctcc actctcttgg ttgcaatgct agccgcttgt ttccatagcc ggtcttgttc
      421 aggggattta gaggattgta gcccaccttg gtcccaggta aattactgaa atgagagact
      481 gaaacgaaag cagaattgtg ttttgttccc tccttttggt taaaaggttg ctaaatcttt
      541 tgatgttcag ggctctgacc cgggggaaaa tattttgtgc ttgttcgctt tccagactaa
      601 tttagttgtc agttaggggt ttccgtcagg ttgacttcac tttatcgtag agccaataat
      661 ggcggacgat tatcaggaat catatgtttt atcggcttaa tttgacggag cgaagtggtg
      721 aaatttcttg ctagtttgat ggggttcttg cttgccttcc agatgacctg tctgcccttg
      781 ccggcagatg cggccgtcga ctacaagcag tctagcggta tgaccggaca ggtggaagtc
      841 aataaagtcg gcgcgctgct tctggctgga ggcgggttgg ctacgttggc cggtaccgct
      901 atcgagatca gtaccaaagg tagtaccagt agcccgaccc tcaaggggca actcttcaat
      961 atgagccagt ccggttcatc cataagcggg cacgctttct tcgacgaagg ctccggtctc
     1021 tcgcaactta gtcccgacgg ctgttcggag tttgtcaagt tgatcgatgg ttccaaggtt
     1081 aacggaccca tttcttccgt caccctcgat gccgttactt gcgccgggca gtctattccc
     1141 tgcagcagaa ttgcctcgat ttcctcggcg cgggtcttta agttcactac caaaatcggt
     1201 gagagcccgc gcatgacctt ccagcccagt tgtgtaaaac tggccggagc ctccaagact
     1261 tatgaagctc ctacctggaa gaagattgtt atcgtcggcg cctgcgtcac agctctagct
     1321 tgtgccatca ctctgccgat tgtgattccg ctggctaccc gcggtaataa ccatcacaat
     1381 gatttgcaaa atgcctatat ctataatact tacctgaatg cttcccgcgt ccaaccgccg
     1441 ccgcagccgg tgagaagcgg cagcggaccg taagtatcca caaatcaatc aaagcgggaa
     1501 aggggcggtg gcaaactgcc cctttcctgt ttcttactac tcaggacttg ccgggctgtt
     1561 tatgcggacc ttccactatg gtggcggggc tcttcgccgc tacccctttg agcatgccgc
     1621 tgaagacgaa ttcgtgcaag ggggttaccg cccaccagta gagcaagcca agcagtccgt
     1681 ggggcacgaa gcgggcaatt tgcctgacct gggtgtgtgt gccctggggg cagatttgaa
     1741 attccaagat ggcttcaccg gggagtttca tctctgccaa caagagcagg tgcttctcct
     1801 cctctacttc tacgacccgc cagaagtcga gggcgtcacc tacgcccagt tcattttcgt
     1861 tgcgcctgcc ccggtttaaa ccgaccccac ccagcatcct atccatgaag cctctcagct
     1921 tccagagcca attgccgtaa taccaaccgg ttttgccgcc gaggcggacc agtctttgcc
     1981 agacttcttt tgtggtatca ctgacggtga tgatgcgact gtcctcgtaa taagtgccgc
     2041 cggcccaacc gggatcaccg ggtgccaacc attcgtctgc aggcatgctg ccggcatcgg
     2101 tccaactgct ttctacagtc tggctgcgca ttctttccag ggctatgccg atagccgtgt
     2161 cggcatccag taaatcttgt ggtatcagct ttagtatctc attgtttttg catactaccg
     2221 ggttgcgcag tccttctgcc agcggacgag cgatgtaggc tggtaccggc gtcacaaagt
     2281 ggatccagta ggaactgatt ctcggggtga aaacaggcac tggcaaaatc actcgtctgg
     2341 gcaaaccggc gcgtcgggca taggtttgca tcaattccat gtaagtgatc acttccggac
     2401 cgccgatgtc gtaagtctca ccggtggtag cttcgatgcc caggcaaccc accagatagt
     2461 tgaggacgtt tcgaatggcg attggttggg agggagtctt cacccagcgg ggagtgatca
     2521 tgaagggtag tcgctccacc aggtagcgca gaatctcgaa ggaagtgctg ccggagccga
     2581 gaatcatgcc tgcccgcagg atggtgacag gcactttgcc gctctttaga atctctccgg
     2641 tctcggccct tgagcgcaag tgcttgctca gttcggggct gtcttccccc agcccgccca
     2701 ggtagataat gcgccgcact ccagcttctt ccgcttctgc tgccatgttt ttggcagcca
     2761 gtcggtctgc ttccgcaaaa tcgcggcttt tggaattcat cgaatgaacg agatagtaaa
     2821 cggcatagac gtccttgagg gcagctttca agtctgctct ttcgagcaca ttgagtgagc
     2881 agagttcaac tccctcttgt ccggcaaagg gtctggcctt gagcttggcc agggagcggc
     2941 cggcggccct gaccagatac ccggcttcca gcagaagtgg gatcaacctt gcacccacgt
     3001 aacccgtggc accggtcact
//
LOCUS       DAOQNZ010000356         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17065_length_3020_cov_3.306239, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000356 DAOQNZ010000000
VERSION     DAOQNZ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17065_length_3020_cov_3.306239"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PKW73_07525"
     CDS             <1..1047
                     /locus_tag="PKW73_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73172.1"
                     /translation="GGKKPDYKGRFIQDLLAAANFKVDPQNIGLVGWDNGGNQALVTL
                     AKFPSELDFISWLGFYESPVGSMFNPPNLGSQQDLLLNKHYREGSASTGNLVVDYRKL
                     KWLAQGFRNPNRMSGKKRGTPGFKGVLYFDENDNGQWEESREFAFNSALDVSMTKQFF
                     PPQVVAACERLKIFGDEWPANVATVSEAEEFFQDRDGSLYVSQVASIYPKLLVGIFAS
                     SVDHAQQQIDHPHITFLYNLFLTNKVRWLRLNPDPQYVAAISSMNYANFVGNRPNTSI
                     DSSTDTGGLLSHLEPEGVVPDYVYMQALIAEMSDRLKTRKLKEFLRAPLVNYSNGAAE
                     PLVPTTPAGRRLQK"
     gene            1137..1424
                     /gene="tatA"
                     /locus_tag="PKW73_07530"
     CDS             1137..1424
                     /gene="tatA"
                     /locus_tag="PKW73_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNA73173.1"
                     /translation="MIFNSPVDVAIVLGVALLVFGPKKLPELGKSLGQGLGNFKKALT
                     DAQEDVTNTVKATPHVESVTLEDRSEKVTDVSAVTSPVAGGGDKQGNPFSG"
     gene            1455..>3020
                     /gene="uvrB"
                     /locus_tag="PKW73_07535"
     CDS             1455..>3020
                     /gene="uvrB"
                     /locus_tag="PKW73_07535"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNA73174.1"
                     /translation="MPEFSVVSSFQPAGDQPRAISELSHGLREGAPFQTLLGVTGSGK
                     TMTMASIIAETQMPALVLAHNKTLAAQLCNEMKEFFPNNAVEYFISYYDYYQPESYIP
                     STDTFIEKEASINEEIDRLRHSTTRSLWERDDVVVVGSVSTIYGLGVPERYLSAAIEL
                     SVGMEIDRHELLKKLVAIHYDRNDLVVERTKFRARGEILEIYPSYEERIIKIAFFGDE
                     VERISFINPVTGEVEEIVNEVRIYPAKHYVADENEIETSVQQIEIELEERIGELIGQG
                     KLVEAQRLKQRTRFDIEMLKEVGYCNGIENYSRILEGRQPGEPPKTLVDYFVRKYGRD
                     GFITFIDESHVTVPQLRGMFHGDRSRKDTLIDYGFRLPCARDNRPLTPDEFFERVGKV
                     VFVSATPGDWELSVSTQVVEQIIRPTGLVDPVVEIRPIDGQIDDLIGEIKERAARDER
                     VLVTTLTKRMAEDLTDYLSQLGIKVKWLHSDIKALERVELLRDLRLGVFDVLVGVNLL
                     REGLDLPEVTLVAI"
BASE COUNT          721 a          771 c          809 g          719 t
ORIGIN      
        1 ggcggtaaaa aacctgacta caaaggtcgc ttcatccagg acttgctggc tgccgctaat
       61 tttaaagttg acccgcagaa tatagggctg gtgggttggg ataacggcgg caaccaggct
      121 cttgtcaccc tggcaaagtt cccatctgag cttgatttca tctcctggct cggcttctat
      181 gaaagcccgg tcggttctat gttcaaccca ccaaatctgg gctctcagca agatctgctc
      241 ctcaataaac actatcggga gggttctgct tcaacaggaa acctggtggt ggactacagg
      301 aagctcaaat ggctggccca gggtttccgc aaccccaatc gtatgtccgg caagaagcgt
      361 ggcactccag gcttcaaagg cgtgctctat tttgacgaga acgataacgg ccagtgggaa
      421 gaatcgagag aatttgcctt caattcggct ctcgacgtct ccatgaccaa gcagttcttc
      481 ccacctcagg tggtggcagc ctgcgaacgc ctgaagatat ttggagatga gtggccggcc
      541 aatgttgcca cggtttccga ggcggaagag tttttccagg accgcgacgg ttccctttac
      601 gtaagccagg ttgcttccat atatcccaag cttctggtgg gcatttttgc ttccagtgtg
      661 gatcacgccc aacagcaaat cgatcatccc cacatcacat ttctctacaa tctcttcctt
      721 accaataagg tgcgctggct gagattgaat ccggatccgc agtatgtagc ggccatttct
      781 tccatgaact atgccaactt tgtgggtaac cgacccaata cttccatcga ctcttctacc
      841 gataccggtg ggcttctcag ccatctggaa ccggaaggag tcgtgcctga ttatgtctac
      901 atgcaggccc tcatagccga gatgtccgat agactgaaga cacgcaaact gaaggagttc
      961 ctcagggctc ccctggtcaa ctattcaaat ggtgctgccg agcccctggt ccccacgacg
     1021 ccggccgggc ggcgcctgca aaaatgaagc ggtcctgtgc tagtatctat taagaacggc
     1081 attaacgatg cgcaccataa gattgcgcta atgccatcgg ttgagaggtt ttgtctatga
     1141 ttttcaacag tcctgttgat gtggctatag tactcggagt agcgcttctg gtcttcggac
     1201 cgaagaaatt gcccgagttg ggtaagtcac ttggtcaggg tctgggtaat ttcaaaaaag
     1261 ccctcaccga cgcccaggaa gatgtaacca acacagtcaa agctactccc catgtagagt
     1321 ctgtcaccct ggaagaccgc agcgagaagg tcaccgatgt aagcgctgtt acaagccccg
     1381 tggccggcgg tggtgacaaa caggggaatc ccttctccgg ctaatctgcc ggcgatttaa
     1441 agtattcaat aagtatgcct gaattcagtg tagtctcgag ctttcagcca gccggagatc
     1501 agcctcgggc tatatcagag ctttctcatg gactgagaga gggagccccc ttccagaccc
     1561 ttctgggtgt gaccggctcc ggtaagacca tgaccatggc cagtatcatc gctgaaactc
     1621 agatgccggc gctggttctg gcccacaaca aaaccctggc cgcccagctc tgcaacgaga
     1681 tgaaagagtt cttccccaat aatgcagtgg agtacttcat ctcttattac gactactatc
     1741 agccggaatc ctatattcct tccaccgata cttttatcga gaaggaagcc agcattaacg
     1801 aagagattga cagactgagg cattccacca cgcgttcgct ctgggaacgg gatgacgtgg
     1861 tggtggtcgg ttcggtttcc acaatttatg gtctgggtgt gccggaacgc tatttgtcgg
     1921 cggccataga gctttccgtg ggcatggaaa tagatcgcca cgaacttctg aagaagcttg
     1981 tggctattca ttacgaccgc aatgatctgg tggtggagcg cactaagttt agggccagag
     2041 gcgaaattct ggaaatctat ccctcatatg aagaacgcat tatcaaaatt gctttctttg
     2101 gtgatgaagt cgagcgaatt tcctttatca atccggtcac cggcgaggtg gaggaaatag
     2161 tcaatgaagt gcgcatctat ccggccaagc actatgtggc ggacgaaaac gaaatcgaaa
     2221 cttccgtgca gcaaattgaa attgaattgg aagagcgcat cggtgaactc attggtcagg
     2281 gcaaactggt ggaagctcag aggctgaagc agcgcaccag gtttgatatc gagatgctga
     2341 aagaagtggg ctattgcaac ggcattgaaa actactctcg catcctggag ggacggcagc
     2401 ccggtgagcc gcccaagact ctggtggact acttcgttcg caaatacggc cgggacggtt
     2461 tcattacatt tatagatgag tcccacgtga cggtgccgca gttgcgcggt atgttccatg
     2521 gggaccgcag ccgcaaagac actcttattg attatggttt tcgtctaccc tgcgccagag
     2581 acaatcgccc cctcacaccc gatgagttct ttgagcgggt aggcaaagtg gtgtttgttt
     2641 cagctacacc gggcgactgg gaattgtccg tctccaccca ggtggtggaa cagatcattc
     2701 gtcccaccgg tctggttgat ccggtggttg aaattcggcc cattgacggc cagattgatg
     2761 atctgatcgg tgaaataaaa gagagagcgg cccgtgatga aagggtgctg gtcactactc
     2821 tcaccaagcg catggcggaa gatctgaccg attacttgag ccaactgggc atcaaggtga
     2881 agtggctgca ctccgatatc aaggcccttg agcgggtgga actcttacga gacctcaggc
     2941 ttggtgtttt cgacgtgctg gttggtgtaa acctcttgcg agaaggtctg gatctgccgg
     3001 aagtgaccct ggtggccatc
//
LOCUS       DAOQNZ010000357         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17092_length_3017_cov_4.013842, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000357 DAOQNZ010000000
VERSION     DAOQNZ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17092_length_3017_cov_4.013842"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="PKW73_07540"
     CDS             complement(<1..1821)
                     /locus_tag="PKW73_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNA73175.1"
                     /translation="MKASEKGKTTSHPTRKPNHLVNETSTYLQQHAYNPVQWYPWGEE
                     ALKRARAENKPILLSVGYAACHWCHVMEHESFEDDTTAALMNEGFINIKVDREERTDI
                     DEIYMKAVQLMTGHGGWPMTVFLTPDLKPFFGGTYFPPLDHHGMPSFKRVLKQIQLAW
                     QNNQDDLMASAGELTEHIQLMERVKADKDSVEESSGADYLNYGTISSALEKLLRNFDS
                     TYGGFGGAPKFPHTFALELCLRAQDRASQVQDSRKADCLNLVKTSLNGMAIGGIHDHL
                     GGGFARYSVDRKWLVPHFEKMLYDNALLARTYLDGYLVTGDKYWLRVARGILSFTKRE
                     LETEAGAFYSSLDADSEGVEGKYYVFTRREIIDILGERDGLWFADIMGVSEAGNFEHG
                     TSVLHLGGHPEKLAKERDLTTEAFLERLEKLSQVVLSAREKRVRPGRDEKVLTCWNSL
                     MLSAFVEAYQVTGEKTYLNTALKGMNFILENLKEGNRLKRVWGKTLDGKGLAKLDGYL
                     DDYACTIQALLDLVSVDAQPRWLNTAIELADSMLTLFQDDKEGGFYYTASDHETLIVR
                     PRSHFDGSVPSGTSSAAMSLLRLFHLTGMERFGKAAQAVLN"
     gene            complement(1818..>3017)
                     /locus_tag="PKW73_07545"
     CDS             complement(1818..>3017)
                     /locus_tag="PKW73_07545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA73176.1"
                     /translation="SKTDFQQPGLTVLTVPKTGSEQLKLAMVVRMFDPAGGGLELYAF
                     KLVQALLQKGHKVTVLCQESKTDFQQPGLTVLTVPKTEKKLSKVERLNYNYEELSKLL
                     AAHRFDLVHSQHCPVKGADLVTFHQHTVKRFSISGDPLERLFNEVKLQLAKPYKLRLK
                     QDEELLKSAKLRIFVSQICRRDYYETYGIKDEVRFPYLIVPPGAERQSPVKTEEEPTT
                     FTFLFIGKGYRKKGLDTLYKACALLKKDGLKFKLLVVGMKDSPGNRMTARLHNLSDEV
                     ELLGFQRDLENIKDRSQVIVVPSKCEAFGMSPIEAMLKGVPAIVSSMAGVTEVLEDGV
                     DSLILHEHLDHHELARHMRKLMEDKRFLSSLKQKAMEKAATVTWENTVELTERAYRQV
                     LKQGAAF"
BASE COUNT          605 a          792 c          815 g          805 t
ORIGIN      
        1 gttcagaaca gcctgggcgg ctttgccgaa gcgctccata ccggtcaggt gaaagagcct
       61 caaaagactc atggcggcag aagacgtgcc ggatggcacg gagccgtcga aatgacttct
      121 gggtctgacg atgagggttt cgtggtcgct ggctgtgtaa tagaaaccgc cctctttatc
      181 gtcctgaaag agagtcaaca tggaatcagc cagttctatg gccgtattca gccacctggg
      241 ctgggcgtct accgaaacca ggtccaggag ggcctggatg gtgcaggcat aatcgtccag
      301 gtagccgtcc agtttggcca gtcctttgcc atcgagagtt tttccccaga cccgtttgag
      361 tcggtttccc tcttttagat tttccaggat gaagttcatg cccttgaggg cggtattcaa
      421 ataggtcttt tcacccgtca cctggtacgc ctctacgaaa gccgagagca tgaggctgtt
      481 ccagcaagtg agaaccttct cgtctcgacc cggtcggaca cgtttctctc tggcgctaag
      541 gacgacctga gagagtttct ccagcctttc cagaaaagcc tctgtggtca aatcccgctc
      601 ttttgccagt ttttccgggt gcccgcccag gtgcaagacc gaggtgccgt gttcgaaatt
      661 accggcctcg ctgacgccca tgatatctgc aaaccagaga ccgtctcgct ctcccaggat
      721 atctataatt tctcttctgg tgaagacata gtatttgcct tctacgccct ccgagtcggc
      781 atccaggctg gagtagaaag cgccggcttc ggtctccagc tctctttttg taaagctcag
      841 aatgccgcgg gcgacgcgca gccagtattt gtcgccggtg accagatagc cgtccagata
      901 ggttctagct agaagggcat tgtcataaag catcttttcg aagtgcggta ccagccactt
      961 acggtctacg gagtaacggg caaagccacc gcccagatgg tcgtggatgc cgccaatagc
     1021 catgccgttt aagctggttt tgactagatt gaggcagtcg gctttgcgag aatcctgcac
     1081 ctgtgaggcc cgatcttgcg ctctcaggca aagctccagg gcgaaggtat gggggaactt
     1141 gggcgcaccg ccgaagcctc cgtatgtaga gtcgaagttg cgcagtaatt tctccagcgc
     1201 actggatatg gtgccataat ttagataatc ggcgccgctg ctttcttcga ccgagtcctt
     1261 atcggctttc acccgctcca tgagttggat gtgttcggtt agttccccgg ccgaggccat
     1321 aaggtcatcc tggttgttct gccaggccag ttgaatctgc ttgagcaccc gtttgaagga
     1381 gggcatgccg tgatgatcca ggggcgggaa ataggtgccg ccgaagaagg gcttcaaatc
     1441 tggcgtgaga aagacggtca tgggccagcc gccgtgtccg gtcatcaact gtacggcttt
     1501 catgtagatt tcatcgatgt cggtacgctc ttcccggtct acctttatgt tgataaaacc
     1561 ttcgttcatg agagcggccg tagtatcgtc ttcgaaactt tcatgttcca ttacatggca
     1621 ccagtggcag gcggcatagc cgaccgaaag taagatcggc ttgttttccg cccgggccct
     1681 tttgagcgct tcttcgcccc agggatacca ttgcacggga ttataggcgt gctgctgcaa
     1741 ataggtgctg gtttcattta caaggtgatt gggcttcctt gtgggatggc ttgttgtctt
     1801 gcccttttcg cttgctttca aaatgccgct ccttgtttca gtacttgtct ataggctctc
     1861 tccgtcaact ccacggtgtt ttcccaggtg acggtggcgg ccttttccat agccttttgc
     1921 ttgagagaag agaggaagcg cttgtcttcc ataagtttcc gcatgtgcct ggccagctca
     1981 tgatgatcta gatgctcatg caagatgaga gaatcgacgc cgtcttccag tacctcggtg
     2041 acgcctgcca tgctgctgac gatggccggc acgcctttga gcatggcttc tatgggcgac
     2101 atgccgaagg cttcgcattt gcttggtacg acgatgacct ggctgcgatc cttgatgttc
     2161 tccagatctc tttgaaaacc gagcaattcc acttcgtcgc tcaggttatg caggcgagcc
     2221 gtcattctat tgccggggct gtccttcatg cccaccacaa gcaacttgaa tttgagcccg
     2281 tcctttttca gtaaggcgca ggctttatag agggtgtcga gacctttttt gcgatagcct
     2341 ttgcctatga ataaaaatgt aaatgttgtc ggctcctctt ccgtctttac cgggctttgc
     2401 cgctctgctc ccggcggcac tatcaagtaa ggaaagcgga cctcgtcctt aattccatag
     2461 gtttcataat aatcgcggcg gcagatctgg gaaacgaaaa tgcgcaactt cgcgcttttc
     2521 aaaagttctt catcttgctt gaggcgaagc ttataaggtt tggccagttg cagttttact
     2581 tcattgaaga ggcgctccag ggggtcgccg ctgatggaaa aacgtttgac ggtgtgttga
     2641 tggaaggtga ctagatccgc tcctttgacg gggcagtgtt gcgagtgcac caggtcgaag
     2701 cgatgtgccg ccaatagttt gctcagctct tcatagttgt aattgagacg ctctactttg
     2761 gagagctttt tctctgtctt cggcactgtt aaaaccgtga gccccggctg ttggaagtca
     2821 gttttgctct cctggcataa gaccgtcact ttgtgccctt tttgcagcag ggcttgaacc
     2881 agcttgaagg catagagttc cagcccaccc cctgcggggt cgaacattct cactaccatg
     2941 gccagcttta gttgttctga ccctgtcttc ggcactgtta aaaccgtgag ccccggctgt
     3001 tggaagtcag ttttgct
//
LOCUS       DAOQNZ010000358         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17093_length_3017_cov_3.574612, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000358 DAOQNZ010000000
VERSION     DAOQNZ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17093_length_3017_cov_3.574612"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PKW73_07550"
     CDS             <1..661
                     /locus_tag="PKW73_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73177.1"
                     /translation="EEVSGLSKLADREKFIAVYPDGNPKSSYSWNNGQWFFSGRDDKA
                     LTSGIMDTLKEKLKVDENRMYIIGYSQGGSFSHRVANELSERVAAVVENGGWMTGKEK
                     QPHKPFPIMSIQSKTDGTVPLDGNPWYSITMKPEVYTQDFYKRAHGIEGAPEVAKRTG
                     KDGTEVEELVWKAPSGAEVKTMLLNNQGHLWYGGKGAEVAPINTTEEAWNFLKRFSRH
                     Q"
     gene            706..1254
                     /locus_tag="PKW73_07555"
     CDS             706..1254
                     /locus_tag="PKW73_07555"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA73178.1"
                     /translation="MALFTRAHLVETRRVHEGDIISLRIDSLEAEDGLSVTREVVEHN
                     GGVVIACEPEPGRVLLVRQYRYSIDKDLLELPAGRIEKDENPLPAAQRELTEETGFGA
                     SHWQELARLYSAPGFCNEVLYLYHATGVTFLGKNLDFDEETEVLDLSLAEAWSLVSQG
                     KIQDAKTVAGLGLMSNRSQSPH"
     gene            1418..1666
                     /locus_tag="PKW73_07560"
     CDS             1418..1666
                     /locus_tag="PKW73_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73179.1"
                     /translation="MANKSDKCCSSLPVAAMFYMAVFSALIVGGSFEVIKYWPLPVSV
                     VPVMGFKGGAIWGALAGGLFGLILGFVTDEKHFEESNS"
     gene            complement(1741..2121)
                     /locus_tag="PKW73_07565"
     CDS             complement(1741..2121)
                     /locus_tag="PKW73_07565"
                     /inference="COORDINATES: protein motif:HMM:NF041106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family (seleno)protein"
                     /protein_id="HNA73180.1"
                     /translation="MTTKQTGKTTIDWRYHRNSCSTCEKAGLYLASHNVEILESVDAR
                     KVRMGRDEALKLAGSVDHIYASRGTKSVHLDLKADKPSDDELIALLIGPTGNLRAPTL
                     KIGRQLLVGFDKVGYDLVFAGSSK"
     gene            2237..>3017
                     /locus_tag="PKW73_07570"
     CDS             2237..>3017
                     /locus_tag="PKW73_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73181.1"
                     /translation="MPSTDGPANLEQGRALCQQGLFLDAVPYFEAALSATERTFGESA
                     PELAEYMQDLGDVYAAVERPADSLRVFDRLYRLGESILGSNHPQIVTVLWKMSILAER
                     LGLVEDSFEMCNQALSRARLCLRPDEELAKAVVERYSYLSNLMREMDEMRRRGEDPLN
                     LQQIENTLEAPRSYQTDLQEPPPAPFRQEERPYEGAVENLGNQYQNVERMPFSPSLND
                     ALGSASVVNLESINRTKALDADLMAQNARAPQQGHLTGSSVF"
BASE COUNT          779 a          780 c          820 g          638 t
ORIGIN      
        1 ggaagaagtg agtggtttga gcaagctggc ggaccgggaa aaattcattg ccgtttatcc
       61 cgacggcaat cccaaaagct cttattcatg gaataacggt cagtggtttt tctcgggacg
      121 agacgacaaa gccctgacca gcggcataat ggataccctg aaagagaagt tgaaggtgga
      181 cgaaaaccgc atgtacatca tcggctattc acaagggggc agcttcagcc accgggtcgc
      241 caacgaactc tcggaaagag tagcggccgt agttgaaaac ggcggctgga tgacaggcaa
      301 ggaaaaacag ccgcacaagc cgtttcccat catgtccatc caatccaaaa ccgacggcac
      361 cgtgcccctg gacggcaatc cctggtacag catcaccatg aaaccggaag tttacacgca
      421 ggacttctac aaacgagccc acggcataga gggcgcgccg gaagtagcca aacgcacagg
      481 caaagacggt acggaagtag aagagctggt ctggaaagca ccgtccggcg ccgaagttaa
      541 gaccatgctg ctgaacaatc aagggcacct ctggtacggc ggcaaagggg ctgaagtagc
      601 gcctatcaat accacggaag aagcctggaa ctttctcaag cgcttctcca gacaccagta
      661 attccagtaa aatttgacaa tctagaaaca gattgaggca ataacttggc attattcaca
      721 agggcacatc tggtagaaac cagaagggtg catgaaggcg acatcatatc tctgcgcatc
      781 gattccctgg aagcagaaga tggtctttcc gtgacgcgcg aggtggtgga gcacaacggc
      841 ggcgtggtca ttgcctgtga accggaacca gggcgcgtct tattggtcag gcaataccgc
      901 tactccatcg acaaagactt gcttgagttg ccggccggtc gtatcgagaa ggatgaaaat
      961 ccactgccgg ccgcccagag agaactgacc gaggagaccg gatttggagc cagccactgg
     1021 caggaactgg ccagactcta ttcggcacct ggattttgta acgaagtgct ctacctctat
     1081 cacgctaccg gggtgacctt cctgggcaag aacctcgatt tcgacgaaga gacggaagta
     1141 cttgacttga gcctggccga ggcctggagt ttagtcagcc agggtaagat tcaagatgcc
     1201 aagaccgtag ccggactcgg tctgatgagc aaccgcagcc agtcgcctca ctgaccggtg
     1261 cgccgaaaag ccgagcttaa aaaactttga tactcgtcct gcccgaaaga agcggcagag
     1321 gcgtacccgg tagcaccccc cttggtgata atataggtga cgattcaatt ccagattcga
     1381 ttctagaaaa taattctggt tctgctagag gtttctcatg gccaataaga gcgataaatg
     1441 ctgttcttcc ctgcccgtag cggcaatgtt ctatatggcg gttttttccg cgctcatcgt
     1501 gggcggttct tttgaagtaa tcaagtactg gcctctgccg gtgagcgtgg ttccggtgat
     1561 gggtttcaaa ggtggagcta tctggggagc ccttgccggt ggtctatttg gactgatcct
     1621 gggcttcgtc actgacgaaa aacacttcga agaatccaac tcctgagcgc ttatacaaaa
     1681 aagctttaga gaggaccctc tatatggacg gtcctctctt tctttgcaat cactagcgct
     1741 ctatttgctg ctgccggcaa aaaccaggtc atagccgacc ttgtcgaagc ccaccaggag
     1801 ctgcctgcca atcttgaggg tgggcgcacg tagattgcct gtgggaccga ttaacaaagc
     1861 gatgagctcg tcatcggagg gtttatcagc ctttaaatcc agatgaacgc tctttgtgcc
     1921 ccgggaggca taaatatggt ccacgctgcc ggccagtttg agagcctcat ccctacccat
     1981 acgcacttta cgcgcatcta cgctttctag aatttcaaca ttatgagacg caagataaag
     2041 accggccttc tcgcaggtgc tgcaagagtt tcgatgatag cgccagtcga tagtagtctt
     2101 gccggtttgc tttgtggtca tagccaaatt cctcaattgc catatataga ctaactagaa
     2161 gtatagacag caacaaactg ggtatacgct aagaagaatg aaaggtttgt ggtgaatgca
     2221 aagcggaaac ttcaggatgc cctctacgga cgggccggct aatctggagc aaggacgggc
     2281 actttgccag caaggacttt ttctcgatgc ggtgccctat tttgaggcgg ctttaagcgc
     2341 tacagaacgc acattcggcg aaagtgcgcc ggagcttgcc gaatacatgc aagatctggg
     2401 tgacgtctat gcagccgtgg aaagaccggc cgactctctg agagttttcg ataggctcta
     2461 tcgactggga gagagcatcc tgggctccaa tcatccgcaa atcgtgactg tgctctggaa
     2521 gatgtccatc ctggccgaac gccttggtct ggtggaagat tccttcgaga tgtgcaatca
     2581 agctctaagt agagccaggc tctgcctgcg tcccgacgag gaactggcca aagctgtggt
     2641 ggagagatac agctacttga gcaatttgat gcgcgagatg gatgagatgc ggcgccgggg
     2701 ggaagatccg ctcaacctgc agcaaataga aaacaccctg gaagcaccgc gcagttacca
     2761 gacagacttg caagagcctc ctccggctcc cttccgccag gaagagcgcc cctatgaagg
     2821 agcggtggag aatctcggca atcagtatca aaacgtagag cgcatgccct tcagtccttc
     2881 cctcaacgat gccctgggta gcgcctccgt agtaaatttg gaaagcatca acagaaccaa
     2941 agcccttgat gccgacctca tggcccagaa tgccagggct ccacaacagg gacatctgac
     3001 cggctcttcc gtattcg
//
LOCUS       DAOQNZ010000359         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17107_length_3016_cov_3.398176, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000359 DAOQNZ010000000
VERSION     DAOQNZ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17107_length_3016_cov_3.398176"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKW73_07575"
     CDS             complement(<1..1005)
                     /locus_tag="PKW73_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73182.1"
                     /translation="MLGVAPAFGEAAEGNVSAELEQIEGSLKRHESKRLEASDYKSLL
                     ERLKVILSESTLEPNQKAKALYLRAMVEFENRDMPGARADIDESLKLSPKNTEALSYR
                     GLINTLTGKPEQALADFTAAIEAGANEPYVFSNRGSVYSQMGKSKEALADFDQAIELS
                     KDDTQTTFARAMRANVLDDLKEYDKAIADCDYVLTRPMPQELLDGIHKRKGGCLFFQN
                     KFKESIEELTLAIEKSSDAKLKASAVFMRGAAYDRLGEKEKALSDLFLARKLGFVTSN
                     PPPDRVVEESPELKAKLVPLTAKARKSLPQVKARFLKGLPSGSFLAVTTTLSGPEGKE
                     R"
     gene            complement(1123..2256)
                     /locus_tag="PKW73_07580"
     CDS             complement(1123..2256)
                     /locus_tag="PKW73_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73183.1"
                     /translation="MDLPHFLMLWLGGTTLAYLLQRAYHEFRWGAFNRANAHLERGCF
                     EEALVAAEAASKRFPKDWRFHFTAAKACIEMQDPEEALAYFRKAEELVAETPIELLIN
                     RAIAKSMLLDYEGALGDINRALQSKQQRLWALLARSYIYHDEYNYQAALDDCNEALNL
                     RATCEAYICRCGVYIGLNNLEAARADLQSLSEHLSKDARPSLVALSLHLQASLLSFDG
                     LFVEAAEEQSKAIELAPQDPFHYINRAYFLSAARQLEEALSDLESADKLAKTKLAHSY
                     IASNRARISIMRDDLDTALQLANEAVAIYERPGILCTRAAIFLLTGALKEAERDLERA
                     QQLNPFDMEIYWWKGRLMEALGREIEAQQNKEKALEGHYKPYF"
     gene            2635..>3016
                     /locus_tag="PKW73_07585"
     CDS             2635..>3016
                     /locus_tag="PKW73_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNA73184.1"
                     /translation="MRELLTPKAKEVIELAEGEAVRFNSRGLSAAHILLGLLLEGTNL
                     GAKALRILGVSVERIHELVEKQDVDHGKSRSLSELIGACSELSGTSGHNYVGPEHLLL
                     FLATDQSSQAFLNTLGIQSDLLRAQ"
BASE COUNT          681 a          719 c          754 g          862 t
ORIGIN      
        1 tctttctttg ccttccggtc cgctcaaggt agttgttact gccagaaaac tacctgaagg
       61 caagcctttg agaaatcgag cctttacctg gggcaggctc tttctagcct tagcggtgag
      121 tggcacaagc ttggctttca gctcagggct ttcttccaca actctatcgg ggggcggatt
      181 gcttgtgaca aaacccagtt tgcgagcgag aaataagtca gatagtgctt tctctttctc
      241 gcccaggcgg tcataggcag caccgcgcat gaagacggct gaagccttca atttggcatc
      301 gctgcttttc tcaatggcca gtgtcagctc ttcaattgac tctttgaact tgttttggaa
      361 gaaaaggcaa ccaccctttc ttttgtggat accgtccaat agctcctggg gcatgggtcg
      421 ggtcagcaca taatcacagt cggcaatagc tttatcgtat tctttcaagt catccagcac
      481 attagctctc atggctctgg caaaggtagt ctgggtgtcg tccttagaaa gttcgatggc
      541 ttggtcgaaa tcggccaggg cttctttgct cttgcccatc tgactgtaaa cggagccgcg
      601 attgctgaag acatagggct catttgcccc ggcttcaatg gctgcggtga agtcggcaag
      661 ggcctgctcc ggtttgccgg tgagggtatt tatcagcccc ctgtagctca aagcttccgt
      721 attcttgggc gagagcttca ggctctcgtc gatatcggca cgagcccccg gcatgtcacg
      781 gttttcaaat tcaaccatgg ctcttaaata gagggcttta gctttttggt tcggctccag
      841 agtggattcc gaaagaatta ccttgaggcg ctccaatagg ctcttgtaat cgcttgcttc
      901 cagtctctta ctttcatgtc ttttgaggct gccctcgatt tgctcgagtt cggccgaaac
      961 gttgccttca gccgcttcac caaacgccgg cgcgacacca agcaaaaaaa ggcatgtgaa
     1021 aatgggtaga actattctca tcgctatatt ttacacttat tcaaatggtg ggcgcggcag
     1081 gtaagatagt tgattgtccg taagacttgc tcaagggcta ttttaaaagt atggcttgta
     1141 atgcccctct aacgccttct ccttgttttg ctgggcctcg atttctctac ctagagcttc
     1201 cattagtcga cctttccacc agtatatttc catgtcaaac ggattaagtt gttgagccct
     1261 ttccagatct ctctcagctt ctttaagcgc accggtcaat agaaaaatag ctgctctggt
     1321 gcagaggatg cccggtctct cgtatattgc gactgcttca ttcgcgagtt gaagggccgt
     1381 gtccagatca tctctcataa tagagatgcg cgctctgtta gaggcaatat agctatgtgc
     1441 caatttcgtt ttggccagtt tgtctgccga ttccaggtct gaaagggcct cctccagttg
     1501 tcttgctgcg gataagaaat aggccctatt tatgtagtga aacggatctt ggggagcaag
     1561 ttcgatggct ttagactgct cttctgccgc ctcaacaaag agtccatcaa aagacaggag
     1621 agaggcttgc aagtgcaggc ttaaagcaac cagagacgga cgggcgtctt tggatagatg
     1681 ttcagataat gattgtaaat ctgctcgtgc tgcttccaga ttgtttaaac caatgtatac
     1741 gccgcatcga caaatgtagg cttcacatgt tgctctcagg ttcaaggctt cgttacaatc
     1801 gtccagcgcc gcttgatagt tgtattcatc gtggtagata tagctcctcg ccagcaaagc
     1861 ccatagtctt tgctgcttgc tttgtaaggc gcgattgata tctcctagtg ctccctcgta
     1921 atcaagaagc attgattttg caatagcccg atttataagc agttcgattg gagtttcggc
     1981 aaccaactct tccgcttttc tgaaatatgc aagagcttct tcaggatctt gcatttcgat
     2041 gcatgctttt gccgctgtga aatgaaagcg ccagtctttc ggaaaccgct ttgaagctgc
     2101 ctcagcagct actaatgctt cctcgaaaca gcctctttct agatgcgcat tggctcggtt
     2161 gaatgcgccc caacgaaatt catggtaggc ccgctggagc aggtaggcta aggtggttcc
     2221 accaagccat aacatcagaa agtgtggaag gtccattcgt gcttactctg cctttgctcg
     2281 gtgtgataaa tgctagttgc acagctccta gtatgttgga caccgctgcg agagcagtct
     2341 tcggatctta gcagtcgctt ttgtggctgt catccatcgg agagaatgcc gggtgcaaaa
     2401 tctccgtggt tttgctgccg gagaggactt ctttggatgg ggtaacactg agaaacaggc
     2461 ggttagggtg aaactctaag aactcgattg gctgcttgaa ttatcggact acccgttgcc
     2521 tctgctttct tctctcggaa ccgggcgaag acttctagcc ttaaagagtg ttcgataaaa
     2581 cgatacactt gttaagtatg cttcaagctc catgttcttt tggaaggtaa taagttgaga
     2641 gagcttttga cgcctaaagc taaggaggta attgagcttg ccgaaggtga ggccgtgaga
     2701 ttcaattctc gtgggttgag tgccgctcac attttgcttg gtcttttact ggagggaact
     2761 aacttgggtg cgaaggcact cagaattctc ggtgtaagcg ttgaacggat tcatgagctt
     2821 gttgagaaac aagatgtgga ccatggtaaa agccggtctc tatcggaatt gataggcgca
     2881 tgttctgaat tgtccggaac ctctggccac aattatgtcg gccctgagca tttactgctg
     2941 tttcttgcta ccgaccaatc cagtcaggcg ttcctaaata ctctcggcat acaatccgac
     3001 ttgctgagag cgcagg
//
LOCUS       DAOQNZ010000360         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17120_length_3014_cov_3.586685, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000360 DAOQNZ010000000
VERSION     DAOQNZ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17120_length_3014_cov_3.586685"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="PKW73_07590"
     CDS             complement(<1..1668)
                     /locus_tag="PKW73_07590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA73185.1"
                     /translation="MELGAEIKSARAILAPGSYIQGKYKIESMIGEGGMGQVFKGVHE
                     ALDKSFAIKVLRSEVVANDKAIRRFDQEAKATSQLSHQNLCQIFDYGLTDGGMPYLVM
                     DLLDGKDLRDVIAEQGPLEPGRVIDLFLAVTDGIDHAHSMGVVHRDLKPSNIIVITTA
                     TKGEAVKVVDFGIAKVMPPPGTESNQLTQTGEIFGSPLYMSPEQCQGLKVDGRSDIYS
                     LGCVMYECLTGKPPFEGANPVQTILKHVNEELKPFDAGLEIPQGLQTIVTRALAKDPD
                     ERYQTVRELHADLQRLRAGEAPSQSARRDSAPMQYLRSRKKMLLLAASIFMLSTVVIV
                     GSAFVQYFQTLSRLSQGANTFASDEPWRLTFEQGKSDQANRNDQYAEEKFRRALEQAK
                     TSSNPELARLQILQRLGDLYIECAEKGQDSYPEARKCFTEAVSLALKSGTLFDKAHTL
                     ERLAEVEYKLKDYSDAEKHFQQALEFREKEPSPIPMSSTLMKQGNNFKRLKKWEAAHQ
                     AYVRAAEYLAKVPGQELFIGNAYKGIAQTNNMMGRYSESDGAFKQAEE"
     gene            complement(1859..2728)
                     /locus_tag="PKW73_07595"
     CDS             complement(1859..2728)
                     /locus_tag="PKW73_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73186.1"
                     /translation="MTDIESDEIIERWLAHNFRPDRHYGAFLTGSTACGASQKGSDVD
                     VVFLTRADAIVDSDSSYHLSYFGGMLFSFTETTQEEALSFCTDPEKAVKYYVRGLQEA
                     QILRDSNRGALERIQKAAQGFYWSDALAAKADERALFHLAGCAEEAQKLTRYLTPAKV
                     RETSTLYLSLLALAINLPIAIALQQRLLCTGEYNLFEQVYGVMGADSLWSRAHRICLG
                     DVSFLSELELPAVLTSKSRARTGLKLYMETARALAPLVEGEEFNLVHAETRKFNAVIE
                     ETMRQLEMLGIWH"
BASE COUNT          746 a          816 c          692 g          760 t
ORIGIN      
        1 ctcttcagct tgcttgaagg ccccatccga ttcggaatat ctacccatca tattgttggt
       61 ctgggcaata cctttatacg cgttgccaat gaaaagctcc tgtcccggta ctttcgccag
      121 atactctgca gctctaacgt acgcctggtg ggcggcttcc catttcttca gtcttttgaa
      181 attattacct tgtttcatca aggtgctcga catcggaatt ggagagggct ctttctctct
      241 gaattccagt gcctgctgga aatgcttctc ggcatcgctg taatctttca acttatactc
      301 aacctctgcg aggcgctcca gggtgtgcgc cttatcgaat aacgtgccgc tcttgagggc
      361 aagggagact gcctctgtaa aacattttct tgcttccggg tatgagtcct gacccttttc
      421 agcacattcg atatacagat cgcccagtcg ctgcaggatt tgcagacggg ctagctccgg
      481 attactgcta gtcttcgctt gctccagggc gcgcctaaat ttttcctcgg catattgatc
      541 attgcgattt gcctgatcag actttccctg ttcaaaggtg aggcgccagg gctcgtcact
      601 ggcaaatgtg ttcgctccct gagataaacg tgagagggtc tgaaaatact gaacaaatgc
      661 tgagccgaca atcaccacgg tggagagcat aaatatactc gcagccaata gcagcatctt
      721 ctttctagac ctgagatatt gcatcggcgc actgtctcgc ctggcagact gagaaggtgc
      781 ctcaccggcg cgcaatctct gcagatcggc atgcaactct ctgactgttt gatagcgctc
      841 gtccggatct ttcgccagtg cgcgagtgac aatcgtttgc aaaccctgcg gaatttccag
      901 accggcatca aagggcttca gctcttcatt cacatgcttg agtatggttt gcacgggatt
      961 ggcgccttcg aatggaggtt taccggtaag gcactcatac atgacacaac cgagagaata
     1021 aatatccgag cgtccgtcca ctttaaggcc ctggcactgc tccgggctca tatatagtgg
     1081 actgccgaat atctccccgg tctgggtcag ctgattgctc tcagtaccgg gaggcggcat
     1141 taccttagca atgccaaaat cgacgacctt taccgcctcg cccttggtgg cagtagttat
     1201 gacaattata ttgctgggct tcaagtcacg gtgaactact cccatgctgt gggcatggtc
     1261 tatgccatcg gtgaccgcca aaaacagatc tatgactctg cctggttcga gaggcccttg
     1321 ctcggcgata acgtcccgca ggtcttttcc atcgagaaga tccatgacca gataaggcat
     1381 gccaccatct gtgagaccat agtcaaatat ctggcagagg ttttggtgag acaattggct
     1441 tgttgctttt gcttcctggt caaaacgtct gatggcttta tcattggcca ccacctccga
     1501 gcgcaaaact tttatggcaa aggacttatc aagcgcctcg tgaacacctt taaaaacctg
     1561 acccataccg ccttcgccta tcatgctctc gattttgtac ttgccctgta tgtaggagcc
     1621 gggagcaaga atcgcccgcg cagatttaat ttccgcccct aattccattg agccgccaaa
     1681 gtcctcctct ttagttcgct caagcccgga ggttgcacaa atacactgcg ccacctgttg
     1741 ttcacaggcg ccacaaacct cattcagccg atttttttct acctgtcggg gctcaaaatc
     1801 caaaataaga acctataaaa gtctctaccc aattgtccca tgtcccttca ggttttgact
     1861 aatgccaaat gccgagcatc tccaactgtc tcatggtttc ctctatgaca gcgttgaact
     1921 tgcgagtttc agcatgcaca aggttgaatt cctcaccttc cacaagaggg gcaagggcgc
     1981 gggccgtttc catataaagc ttaagccctg tcctggcccg acttttactt gtgaggacgg
     2041 caggcaattc caattcagaa aggaaggaaa catcacccag acaaattctg tgggcacgac
     2101 tccagagcga atctgcgccc atcacaccat aaacttgctc aaatagattg tattcccccg
     2161 tgcaaagaag tctttgctgg agagcaatag ctatgggcaa gttgatagcc agcgccagca
     2221 gagacaaata gagggtgctt gtttcacgga ccttagcagg agtcagatat ctagtcagct
     2281 tctgagcctc ttcagcacaa ccggcaagat ggaaaagggc acgctcatct gctttggcgg
     2341 caagagcgtc gctccaatag aacccttgag ccgccttctg aattcgctcc agcgcccctc
     2401 gattggagtc cctgagaatc tgcgcctctt gcagcccccg aacataatat ttgacggcct
     2461 tttccggatc ggtgcagaag ctcaatgcct cttcctgagt agtttctgta aatgagaaga
     2521 gcataccgcc gaaatacgag agatgatagc tagaatcact atcaacgatg gcatccgctc
     2581 tcgtcaaaaa gaccacatct acgtcactgc ccttttgcga cgcaccacaa gcggtgctac
     2641 cggtaaggaa agcgccgtaa tgacggtccg gtctaaaatt gtgcgccaac cagcgttcaa
     2701 taatttcatc cgattcaata tccgtcattg tgctttactt aaaactggtg gtcgaaagta
     2761 gtctaaaata tgcaaggaat actcagcctc caacatagca ccagcccgag gagagaggga
     2821 cagaaccact acttcaaaag tgaatccgct ggactaaatt atatcccaac agtcactccc
     2881 acagccaaga ataaaatcca ggcagcggca ggcggctgca acgtaaacaa tcacaaaagg
     2941 ctattcaaga acctttgaag cgtaatttat gggcattatt cattgcactg acggttatat
     3001 gtagtaccgc cggg
//
LOCUS       DAOQNZ010000361         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17139_length_3012_cov_3.898884, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000361 DAOQNZ010000000
VERSION     DAOQNZ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17139_length_3012_cov_3.898884"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..870)
                     /gene="eutC"
                     /locus_tag="PKW73_07600"
     CDS             complement(136..870)
                     /gene="eutC"
                     /locus_tag="PKW73_07600"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HNA73187.1"
                     /translation="MKDREKDEQLSEHLSRFTPARINVGKAGNRPRTSTWLQFRADHA
                     LAKDAVSSNFSERFLKTFAAQYELPVMETLAKSREEFLLNPPLGKKTSNDILDEIVKI
                     LPTGWDVLIVISDGLSSHAVEENLPDLYPMLLDGFDQAGISTSKGLLVKQGRVAIADQ
                     VAHALGARVALNLIGERPGLSTASSLSAYITYMPGPQTISSDRTVISNIHKGGTPAVE
                     AGAFIVKTIARILEKKASGVRLQALG"
     gene            995..2365
                     /locus_tag="PKW73_07605"
     CDS             995..2365
                     /locus_tag="PKW73_07605"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNA73188.1"
                     /translation="MNLSVKLGGRQFHFHSVKEVLAKANEEKSGDRLAGIAAQSELER
                     VAAKNCLSSLTLEELRANPVLPAEDELTKLVDAALDEAVFLGIKNWTVAELREWLLNE
                     ATGAADIAAIRPALNGEMAAAVTKLMSNMDLVLAARKATVVVKAKTTLGLPGRLSARL
                     QPNHPSDSIEGIRASIFEGLSYGAGDAVIGINPVFDTADTVARLLDDTATLIEKLAVP
                     TQNCVLSHITTQIKAIERGARAGLIFQSIAGSEAGNRAFGIDAALIQEAYELGSTQHK
                     DDELFMYFETGQGSELSSRTHNDADQVTLEARCYAFCRLYNPFLINDVVGFIGPEYLA
                     SGKQMIRAGLEDHFMGKLLGLPMGVDACYTNHMDSDQNDLENLTLLLTAAGVNYFMGV
                     PMGDDVMLSYQSTSFHDIASLREVFNLRPAPEFEAWLENVGLMKNGRLTKHGGNPSYL
                     LQGSFA"
     gene            complement(2377..>3012)
                     /locus_tag="PKW73_07610"
     CDS             complement(2377..>3012)
                     /locus_tag="PKW73_07610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA73189.1"
                     /translation="LAAPEALAQGRGRDPVVLLSTSKGPIAIRVFAGMVPRTASNFLD
                     LVNRGYYDGKIFHRVENWVVQGGCPYGNGRGNFVDPDTGETRFIPLEINRNLGHGQAG
                     MVAMARSADPNSASCQFYILKQPMPRLNGQYAVFGMVVGGLQNIYRIGIGDRILSARL
                     DAPAESQSESNGGSEPAGTGRGTTRETSESTSPSSGQGKSGGASGGSESGF"
BASE COUNT          648 a          763 c          836 g          765 t
ORIGIN      
        1 ttgttattga gctattgggc aggcgattga agatttgctg attgtccacc ttcagcctgg
       61 ttaagaccat gtgagtggtg gtggtaccaa catcgatgcc gaccgataaa agcgagttgc
      121 cgtctttatt ttgcatcagc cgagggcttg caatctcacg ccggacgctt ttttctctaa
      181 aatgcgtgcg atggttttga caatgaaggc gccggcttcc actgccggtg ttccgccctt
      241 gtgaatattt gatatgacgg tgcggtccga acttatggtt tgaggaccgg gcatgtaagt
      301 gatgtaggct gacaagctgg aagccgtgct cagacccggt ctttctccaa tcaaattgag
      361 tgctacccgg gctcctaagg catgggctac ttgatcggcg atggccacgc ggccctgttt
      421 tacaagcagt cctttgctgg tgcttatgcc ggcctggtca aaaccatcta gcagcatggg
      481 atagaggtcg ggcagattct cttccactgc atgggaggag agaccgtcgc tgattacaat
      541 cagtacgtcc cagccggtcg gcaaaatctt gacgatttca tcgagaatgt catttgatgt
      601 ctttttgcca agcggcggat tgagcagaaa ttcttctctt gatttagcca gtgtctccat
      661 tacgggcagc tcatactgtg cggcaaaagt tttcagaaaa cgctcactaa aattgctgga
      721 tactgcatct ttggccaggg catggtcggc acgaaactga agccaggtgc ttgtgcgtgg
      781 tcggtttccc gcctttccca cattgattct ggctggtgta aatctgctca ggtgttcgga
      841 gagttgttcg tctttttctc tgtctttcat ggcttccggc atttctattc gacctgagtc
      901 gatctcagtt gggctgagta aagcaacagt aacgatgcaa taggttacac tatcgtgtcg
      961 ttctttatct aagtcgaaag caaatggtaa tagcgtgaat ctttcagtca agcttggcgg
     1021 caggcaattt cactttcact ctgtgaaaga agtgctggct aaggccaatg aggagaagtc
     1081 aggagacagg ttggcaggca ttgccgctca gtcggaattg gagcgagtgg ccgccaagaa
     1141 ctgtctttca tcgctaacct tggaagaact gcgggccaat cctgttttgc cggctgagga
     1201 cgaactgaca aagttggtag atgccgccct tgatgaagca gtctttctcg gcataaaaaa
     1261 ctggacggtt gccgagctga gagagtggtt gttgaatgag gctaccggtg cagccgatat
     1321 cgccgccatc agaccggcct tgaacgggga gatggccgct gccgtgacca agctcatgtc
     1381 caacatggat ctggtgctgg ctgcccgcaa agcgaccgta gtggtgaaag ccaagaccac
     1441 actgggcttg cccgggcgac tttccgcccg tctgcaacct aatcatccct ccgattctat
     1501 agagggaatt cgcgccagta ttttcgaagg gctctcatat ggtgccggag acgcggtgat
     1561 cggtataaat ccggtttttg atacggctga taccgtcgcc aggttgctcg acgacacggc
     1621 gaccttgatt gagaagttgg ctgtgccgac acaaaactgt gttctctctc atatcaccac
     1681 gcagattaaa gctatcgaaa ggggcgccag ggccggtctc atttttcaga gcattgccgg
     1741 tagcgaggcc ggaaaccgtg ctttcggtat agatgccgcc ttgattcagg aagcttatga
     1801 gcttggttcg actcagcaca aggacgacga actttttatg tatttcgaaa ccggtcaggg
     1861 ttccgaatta tcttcccgca cacataacga cgcagaccag gtgacgctgg aggccaggtg
     1921 ctatgccttc tgccgcctat acaatccctt cctgataaat gatgtggttg gttttatcgg
     1981 tcccgagtat ctggccagcg gcaagcagat gataagagcc ggactggaag atcatttcat
     2041 gggcaaactt ctgggcctgc ccatgggtgt cgacgcttgc tacaccaatc atatggattc
     2101 ggatcaaaac gatctggaga acctgacgct cttgcttacc gctgccggcg tcaattactt
     2161 catgggtgta ccgatgggtg atgatgtgat gctttcttat cagtcaacca gctttcacga
     2221 catcgcttcc ctgagggaag ttttcaattt gagaccggcg cctgagtttg aagcgtggtt
     2281 ggaaaacgtt ggtttgatga aaaatggacg cctgaccaag catggaggta atccctctta
     2341 ccttctccag ggcagtttcg cctgagtaaa tcgaattcag aagccgcttt cggagccgcc
     2401 gctggcaccg ccgcttttgc cctgaccgct ggaaggggag gtgctttcgc tagtctctct
     2461 agtcgttcct ctgccggttc cagccggctc ggatccgccg ttgctttcgc tctggctttc
     2521 cgcaggggcg tccagtcgcg ctgaaagaat acgatcgcct atgcctatcc ggtagatgtt
     2581 ttgcagtccg cccacgacca tgccaaacac cgcatattga ccatttagcc gcggcattgg
     2641 ttgtttgaga atgtaaaact ggcagctggc ggaattggga tcggccgaac gggccatggc
     2701 caccatgccg gcctgtccgt gaccaagatt acggttgatc tccagaggaa tgaaacgggt
     2761 ttctccggta tccgggtcga cgaagttgcc tcgaccgttg ccgtaggggc agcccccttg
     2821 cactacccag ttttccaccc ggtgaaaaat cttaccgtcg tagtaaccgc ggttgaccag
     2881 gtcgagaaaa ttggacgctg tgcgtggaac catgcctgcg aagacacgaa tggcgattgg
     2941 tcccttgctg gtactcagta agaccaccgg gtctcttcct cgtccctggg ccagggcctc
     3001 gggagcggcc ag
//
LOCUS       DAOQNZ010000362         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17141_length_3012_cov_3.195807, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000362 DAOQNZ010000000
VERSION     DAOQNZ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17141_length_3012_cov_3.195807"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1252)
                     /locus_tag="PKW73_07615"
     CDS             complement(44..1252)
                     /locus_tag="PKW73_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNA73190.1"
                     /translation="MSGNDQLPLPYGALSSYPLSGCEDGPLKGLTFAVKDVFALRGHV
                     NSYGCPDWKLSHDPSPFTQAAVLRLLETGADLRYVALTDELAFSLDGINAHYGTPLNP
                     ACPERIPGGSSSGSASLTAGGFVDFALGTDTAGSVRVPASYCGLYGLRPTHGLIELEG
                     VLPLGPSFDTVGVLSSTLPVLSAVAEVLLVASGISDVSDDGGAAAAKLFIDERARAYL
                     PPSLRTVFDAEIEGIAKAFKVVERGEGPFDMAELSRHFAAVRGYEAWLQHRQWYETVH
                     PRLSEVVQERLIKCSQVEESDCLASQAYRQDLRLRLQHWFSDGRVLVLPTTCAPAPLL
                     VASSEQLLENRLKNLDLNALASVAGLPQLTLPRLHVSAGDGNRAPLGLSLVGGVGQDL
                     NLLAIAAELP"
     gene            1608..2624
                     /gene="argF"
                     /locus_tag="PKW73_07620"
     CDS             1608..2624
                     /gene="argF"
                     /locus_tag="PKW73_07620"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNA73191.1"
                     /translation="MSLTTKPLALNMNKPQRMAKPSGTSPLAFVLEEHSLRGKDITGI
                     ENLSISELNLILDVAAKLKENKFDETQTLFGKGQTLAMLFEKPSLRTRVTFEAGMTQL
                     GGHAIYLEGKLGERESVNDIGKNLERWVDGIMARTFEHETVVALAANANVPVINGLSN
                     KEHPCQALADFQTLIEHKGNIKGLKMAYVGDANNVSNSLLLLAASLGTDFAMACPHKY
                     EPQASIWMKALELAKISGANLTLVEDPKLAVEGADAVYTDVWTSMGQEAEAKEREKIF
                     APYQLNGNLLKQAKEDALVMHCLPAHRGLEITDEVIDGPNSVVFDQAENRLHAQKAIM
                     ALVL"
     gene            2680..>3012
                     /locus_tag="PKW73_07625"
     CDS             2680..>3012
                     /locus_tag="PKW73_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNA73192.1"
                     /translation="MKKILLAYSGGLDTSCILTWLKEKYQVPVVAYCANVGQEEDFEQ
                     VHAKALKTGAEKCIIDDLKAEFVKDFIFPSIQANAVYETVYLMGTSLARPCIAKGMVE
                     AALREGCDF"
BASE COUNT          822 a          858 c          751 g          581 t
ORIGIN      
        1 ttgagtcgtt tctaaagacg agtagaccgc gaaagccgcc gggttagggc agttccgcag
       61 caatggcaag caaattgaga tcttgcccga caccccccac tagtgaaaga ccaagcgggg
      121 cacgattgcc gtcaccggct gaaacatgca agcgcggcag tgtcaattgc ggcagtccgg
      181 caacggaagc aagcgcattc agatcaaggt tcttcagtct gttctcaagc agttgctccg
      241 aagacgccac taatagcggt gcgggcgcgc aggtagtcgg cagaaccaaa acgcgcccat
      301 cggagaacca gtgctgcagc cttaagcgca agtcctggcg ataagcctga ctggccagac
      361 aatcgctttc ctcgacctgg gagcatttaa tcagtcgttc ttgcaccacc tcagacaagc
      421 gcggatgcac agtctcatac cactggcggt gctgcaacca ggcctcatag cctcttactg
      481 cggcgaagtg ccgggacaat tcagccatat caaaaggacc ttcgccccgc tcaaccacct
      541 taaaagcttt tgcaattcct tcaatttccg catcaaaaac agtgcgaagc gaaggcggca
      601 agtaagcccg tgctctctcg tctataaata acttggcggc ggcagcacca ccgtcatcac
      661 ttacgtcact aatcccggaa gcaacaagca agacctctgc caccgcactt aaaaccggca
      721 aagtggaaga caagacgcct accgtatcga agctcggacc aagaggtaag acaccttcca
      781 gctcaatcaa cccatgggtg gggcgcagac catagagtcc gcaataggaa gccggcaccc
      841 gcacagagcc tgccgtatcc gtaccgaggg cgaagtctac gaaaccaccg gcagtcaggg
      901 aagccgagcc actggaagat ccgcccggga tgcgctcggg gcatgccgga ttgagcggtg
      961 tgccatagtg agcattgatg ccgtccagac tgaaggccag ctcatcggta agggccacat
     1021 accggaggtc ggcaccggtc tctaagagcc taagcaccgc tgcctgggtg aaaggagatg
     1081 gatcatggct gagcttccag tcaggacaac cgtaactatt aacatgtccc cttagagcga
     1141 aaacatcttt gacggcaaag gtaagaccct taagcggccc gtcctcacaa ccagagagcg
     1201 gataggagga cagagcacca tacggcaaag gcaattgatc atttccagac aaaggcaatg
     1261 ctcccgacaa taaatcacaa gcaactccta cataactatg gcagaatttt tcgtgtgaag
     1321 catcctgcaa catttgctgc cgatggggca aattttcgtt gacaaagctt tcgttcgact
     1381 gtaactttca atggtcgatg tttttcagca agccgttttg cggcaaggat ctgcactcta
     1441 atgcgcgtca actccaaagc attaatagcc gtatccagcc gccgccgccg ccagttctca
     1501 acgaaccggg gcataatctt gtaagcgcaa ctgcaaaacc aagacttgag catccccggc
     1561 aaacaccggg gatttttttt acaccaggaa ataaggagag agactagatg agcctgacca
     1621 caaagccctt agcgctgaac atgaacaaac cacagcgcat ggccaagccc tcgggcacat
     1681 cgcctttagc ctttgtgctg gaagaacaca gcctgcgcgg caaagacatc actggcatcg
     1741 agaacctctc aattagcgag ctcaatctca ttctcgatgt tgccgccaaa ctgaaagaaa
     1801 acaaattcga tgaaacccag accctcttcg gtaagggaca gaccctggct atgctctttg
     1861 aaaagccctc actgcgcacc cgagttactt tcgaagcggg aatgacccaa ctgggaggtc
     1921 atgccattta cctggaaggc aaactgggag aaagggaatc cgttaacgac atcggcaaaa
     1981 atctggagcg ctgggtggac ggcatcatgg cccgcacctt cgagcacgaa accgtggtgg
     2041 ccctggccgc taatgccaat gtgccggtga tcaacggtct ttccaacaaa gagcatccct
     2101 gccaggccct ggccgacttt caaaccctta ttgaacacaa aggcaatatc aaaggtctca
     2161 agatggctta tgtcggagac gccaacaatg tctccaattc cttgctctta ctggccgcca
     2221 gcctgggcac agacttcgcc atggcctgcc cgcacaagta cgagccccag gcatccatct
     2281 ggatgaaagc cctggagttg gcaaaaatct ccggagcaaa cctcacccta gtggaagatc
     2341 ccaaactggc agtggagggt gccgatgccg tctacaccga tgtctggacc tctatgggtc
     2401 aggaagcgga agcgaaagaa agagaaaaga tcttcgcgcc ttatcaactg aacggcaacc
     2461 tgctcaagca ggccaaagaa gatgccctgg tcatgcactg cctacccgcc caccgtggtc
     2521 tggaaatcac ggacgaagtg attgacggac ccaattctgt tgtctttgat caagcggaaa
     2581 accgcctgca cgctcaaaag gccattatgg ctctggtcct ctaaagcgat tcagactgac
     2641 tttcattcaa actctccgac ctcagtaagg aaccaaaaca tgaagaaaat tctcctagct
     2701 tattcaggcg gtctcgatac ctcctgtatc ttgacctggc tcaaagaaaa atatcaggta
     2761 ccggtagtag cctactgcgc caacgtgggg caggaagaag acttcgaaca ggtacacgcc
     2821 aaagctttga aaaccggagc cgagaaatgc attatcgatg atctgaaagc ggaattcgtc
     2881 aaagacttca ttttcccgtc cattcaagcc aacgccgtct atgaaaccgt ctacctgatg
     2941 ggcacctccc tggcccgtcc ctgtatcgcc aaaggcatgg tggaggcggc ccttagagaa
     3001 ggttgcgact tc
//
LOCUS       DAOQNZ010000363         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17213_length_3005_cov_3.755932, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000363 DAOQNZ010000000
VERSION     DAOQNZ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17213_length_3005_cov_3.755932"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2120
                     /locus_tag="PKW73_07630"
     CDS             <1..2120
                     /locus_tag="PKW73_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73193.1"
                     /translation="PEGKSVSLWKCYFLGLALLLSALFVFYQPVLLGGRDYFVSDHTY
                     FFEPFARFIEEGLRRGRLPSWNPYLYCGMSQLANPSPGIFYFPTALFAFLPYSQSMAL
                     TLALSQVVAYGAGFLMAVELSLPAAAACALGLMLALNGYMFSLSANYSLPATAAWGFL
                     TVSLFLIMMRSDGRKRLTYMALSALTCHLTIMAGRPEIFIPIFSVLAVVALAPLRRLK
                     QENMGRVLTAFLFPAASFALAILMSMVSIAPGYEWTKLSPRASGLALSQVFLWSANWY
                     DFICVFTGQPLGDLQHALSRFLSVVTSRKGYFPFLPTAHVGSVVLLLACLSLAYPGHK
                     LRRLLLLAGFLIAACLAAGDNTPLSRLVLAAVPPLAVLRYPVKLLVFSNYFLCLLAAH
                     GFAQTVSDSYSSELKRLIKLLCAVFLSLSLLFGLAYLLRDFYGGQTFKIDALAVKEFT
                     LSPPAFFAMLFKSLFLSAFIAFLLSLVLAFSERLKLGSREKTALIMGALLGSILTPSY
                     QMPPRTVAADFYRNVPLADKLEALSQEPGSESFAVKGRGRYLTCYFDPLVVAKGYVPR
                     QPNQFGETYMQYCRELLLPNTNVSLNYPVTFGYEAAETKDYHKAFLDCLHKSSVDVKG
                     ASDLPLYRFCLITSTRFLGTQIKGKKGPSNLMNPIFFELIEENKAYNRRLYKVRQAGD
                     RYYVSKRYREAKSVAETTAWLLS"
     gene            2136..2705
                     /locus_tag="PKW73_07635"
     CDS             2136..2705
                     /locus_tag="PKW73_07635"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNA73194.1"
                     /translation="MTPEAVHDSFVDLIETAPDIGSVFEQDNFFYRDMEDEKDLERLQ
                     MASLNRSVTGLTPGRRPLVSSAKLLTDEDNHLTFSVSMEKDGYFVLNDRFYPGWHVKV
                     DSLPAKLLRANGFMRAVHLNAGSHLVEFDFRPKSFSFGLFLTAVGFALTLVCLLVGLA
                     PLIWQGILFLSYGSSRGKAATASQGVQEQ"
     gene            2765..2841
                     /locus_tag="PKW73_07640"
     tRNA            2765..2841
                     /locus_tag="PKW73_07640"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2799..2801,aa:Arg,seq:cct)
BASE COUNT          575 a          860 c          759 g          811 t
ORIGIN      
        1 accccgaggg gaagtcggtt tccctctgga agtgctactt ccttggactg gcgctgctcc
       61 tttcggccct tttcgtcttc tatcagccgg tcctacttgg cggcagagac tatttcgtct
      121 ccgaccacac ctatttcttc gagcccttcg cccgctttat cgaagagggg ctgaggcgag
      181 gtcgtctacc ctcctggaat ccctatctct actgcggcat gtcccagttg gccaaccctt
      241 cgccgggaat cttctacttc cccaccgctc tctttgcctt cctgccctac agtcagtcca
      301 tggctctgac cctggccttg agccaggttg tggcttacgg tgccggtttt ctcatggcgg
      361 tggaattgtc tttgccggca gcggccgcct gcgccctggg tttgatgctg gccttgaacg
      421 gctatatgtt ctccctgagc gctaattaca gccttcctgc caccgccgcc tggggctttt
      481 tgacggtctc cctcttcctc atcatgatgc gctccgatgg gcgcaagcgc ctcacctata
      541 tggcactttc cgctctcacc tgccacctga ccataatggc cggacgaccg gagatcttca
      601 ttcccatctt cagcgtgctg gctgtagtag ccctcgcccc cctgcgacgg ctcaagcaag
      661 agaacatggg cagggtactt acggcctttc tttttccggc tgcctccttt gccctggcaa
      721 tactcatgag tatggtctcc attgcccccg gctatgaatg gaccaaactc tctccccgcg
      781 ccagtggact ggccctcagt caggtttttc tatggagcgc caactggtac gacttcattt
      841 gcgtattcac aggccagccc ctcggcgacc tgcaacatgc cttgagccgc ttcctttcgg
      901 tggtaaccag ccgcaaaggc tatttcccgt ttctgcccac ggcccatgtg ggctcggttg
      961 ttcttttgct tgcttgtttg tcgctcgctt atcccggaca caagctgcgg cgccttttgc
     1021 ttctagccgg ttttttgatt gctgcctgtc tggctgccgg cgacaacacc cccttgagcc
     1081 gactggtgct ggcagccgta ccaccactgg cagtgctgcg ctacccggtc aagctacttg
     1141 tcttttccaa ttactttctt tgcttgcttg ccgcccacgg ttttgcccag accgtttcgg
     1201 atagctattc cagtgagcta aagcggctga taaagctgct ttgcgcagtc tttctttccc
     1261 tcagcctgct ctttggtctg gcctatttgc tgagagattt ttacggtggt caaacattca
     1321 aaatagatgc cctggcagtg aaggaattta ccttaagccc gcccgccttt tttgccatgc
     1381 tcttcaaatc gcttttcttg agtgccttta tcgccttctt actgagtctg gtactggctt
     1441 tcagcgagcg cctcaaactg ggcagtcggg aaaaaacggc tctcatcatg ggggccctgc
     1501 tcggctccat tcttacaccc agctatcaga tgccgccgcg gacggtggca gccgatttct
     1561 atcgaaacgt acctctagcc gacaagttgg aggcgctcag tcaggagcca ggcagcgaga
     1621 gctttgccgt gaaagggcga ggccgctacc tgacctgcta tttcgacccc ctggtggtgg
     1681 ccaaaggtta tgtgccgagg cagcccaatc agttcggcga aacctatatg caatactgcc
     1741 gggaactact tttgccaaat actaatgtct cgcttaatta cccggttacc tttggttatg
     1801 aggcggctga aacaaaggac taccacaagg cttttctcga ttgcctgcac aagagttcgg
     1861 ttgatgtaaa aggggcttcc gaccttcctc tttaccgctt ttgtctaatc acttccaccc
     1921 gctttctggg cacccagatc aagggcaaga agggtccgtc taatcttatg aatcccatat
     1981 tcttcgagct aatcgaggag aacaaagcct ataaccgcag gctctataaa gtgcgtcagg
     2041 ctggtgaccg ctattatgtc tcgaagcgct atcgggaagc taaaagtgtg gctgagacta
     2101 ccgcctggct cttgagttag caaccgcaag aggctatgac tccggaggct gtacacgata
     2161 gttttgtgga tttgattgaa acggcgccgg acatcggcag tgtttttgag caggataatt
     2221 tcttctaccg ggatatggaa gacgagaagg acctcgaacg tctccagatg gcttcgctga
     2281 accgctccgt caccgggctt acacccggac gtcgacccct tgtttcatcg gcgaagctcc
     2341 tgaccgatga ggacaatcac ctgaccttca gtgtcagcat ggaaaaggac ggctatttcg
     2401 tcctgaatga ccggttttat cccggctggc atgtgaaagt cgatagtctg ccggcaaaac
     2461 ttctaagggc caatggcttc atgcgggccg tgcacctcaa tgccgggtcg catctggtgg
     2521 aatttgattt tagacccaag tctttctcat tcgggttgtt cttgactgca gtgggctttg
     2581 ccctcaccct ggtctgcctt ctggtggggc ttgcgccgct aatctggcag ggcattctct
     2641 ttctttccta tggttccagc aggggcaagg cagcgacagc ttctcaaggg gttcaagaac
     2701 agtaacttaa ccaaaggagc cggggctaat tctagtattt ccttggtaga ctatctacta
     2761 tcatgccctc gtagttcagt tggatagaac acctccgtcc taaggagggt gtcgggagtt
     2821 cgagtctctc cgagggtgcc aattctgttt gtcttccaag acttctagcc aattgtcgat
     2881 tttcttgtaa gtgcggggta agtgcacttg aaattttagg aggcgagttt tgtacagcca
     2941 ggcatattcg gaaaccgatt accaagatga actgaaaagt ggaaagattc cagatgaata
     3001 cgccc
//
LOCUS       DAOQNZ010000364         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17258_length_3001_cov_3.876782, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000364 DAOQNZ010000000
VERSION     DAOQNZ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17258_length_3001_cov_3.876782"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PKW73_07645"
     CDS             complement(<1..1009)
                     /locus_tag="PKW73_07645"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HNA73195.1"
                     /translation="MSNPHGIKHNFLDLNAKPGEDFFRFANGAFIDSAQLPPGHARWC
                     MFDEVNEQAQTAIKAIMTEAAELGEQGSIEQRQIGDFYLSGMNLEQIEAEGLTALAPL
                     LSMINKSGTPRTFARALGHMHMVGMAPFFGFGSMQSFADSKQQIAATGQAGLSLPDRS
                     YYLKKTKKNLELMRQFEQHVVNMFVLMGESKPRAMRFAAAVCRLETTLARASMPQEDM
                     RNPNNINHVMTVDELKALVPHFDFDTYFQTIGAPAFSSLNVMQPKFFKAFSRVLSKLT
                     KLERIAYLKWHLISTAAPKLGSAFVDEDFAFFGKIMQGKTSQLPRWKRVVATVNGLLG
                     EA"
     gene            complement(1080..2090)
                     /locus_tag="PKW73_07650"
     CDS             complement(1080..2090)
                     /locus_tag="PKW73_07650"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNA73196.1"
                     /translation="MKLATVKNGTRDGMLVLVNRTMTKALPIPQIAATLQYFLDNFQQ
                     LEKQAKQEFERLEAGQAAGAIDFEPGNCLSPLPRAYQWLDGSAYLNHVELVRKARGAE
                     MPESFLTDPLMYQGGSDAFLAPLEAFPLASEAYGADMEAEVAVIVDDVPMGISAEAAA
                     AHIKLVMIVNDMSLRELIPSELAKGFGFVHGKPSSAFAPVAVTPDELGQAWKDAKLHL
                     PLLTYLNGQLIGSPNAGCDMNFSMAELIAHAAKTRALSAGTIIGSGTVSNRDRKTGVS
                     CLAEKRMIEKIESGAPVTPFMKFSDVVRIEMLDQNGKSIFGAIEQKIVAATPAPTLLT
                     TK"
BASE COUNT          631 a          729 c          852 g          789 t
ORIGIN      
        1 ccgcttcccc cagcaagccg ttcacggtag caacaactct cttccagcgc ggcaactggc
       61 tggtctttcc ttgcataatc ttgccgaaga aggcgaagtc ttcgtctacg aaggcgctgc
      121 ccaacttggg cgctgccgtg ctgatcaagt gccatttcaa gtaggcgatg cgttccagct
      181 tggtcagctt ggagagcacc ctggagaagg ctttgaagaa ctttggctgc atgacgttca
      241 agctagaaaa agccggtgcg ccaatggttt ggaagtaagt gtcgaagtcg aaatggggca
      301 ccagtgcttt cagctcatca accgtcatga catggttgat gttgtttgga ttgcgcatgt
      361 cttcctgggg catagaggct ctggccagtg tcgtctccag cctgcagacg gcggcagcaa
      421 agcgcatggc gcggggcttt gattcgccca tgagaacgaa catattgacc acgtgttgtt
      481 cgaactggcg catcagctcc aggttcttct tggttttctt caggtagtag gagcggtccg
      541 gtaggctcaa tccggcctgc ccggtggcgg caatctgttg cttgctatcg gcaaaggatt
      601 gcattgagcc gaagccgaag aagggggcca tgccgaccat gtgcatatgt ccgagggcac
      661 gggcgaaggt tctaggcgtg ccgctcttgt taatcatgct cagtagcggc gccagggccg
      721 tcagaccttc cgcctctatt tgttccaggt tcatgcccga aagatagaaa tcgccaattt
      781 gtctttgctc gatactgccc tgttcaccaa gttctgccgc ttcggtcatg atggctttaa
      841 ttgcggtctg ggcctgttcg tttacctcgt cgaacataca ccagcgggca tgaccgggag
      901 gcagttgagc gctgtcgata aaggcgccgt tggcgaagcg aaagaagtct tcaccaggct
      961 tggcattcag gtctagaaag ttatgtttga ttccgtgagg gttgctcata tctattactc
     1021 caaaaaggaa gacccggcag cattagcggc cgggtctaag ggttaagtgg gtgagggcgt
     1081 tatttggtgg tgagcaaagt tggggcaggt gtggctgcca caatcttttg ttcgatggca
     1141 ccaaagatgc tcttaccgtt ttggtcgagc atttcaatgc gaaccacgtc cgagaatttc
     1201 ataaacggtg ttaccggggc accgctctcg attttttcaa tcatgcgttt ttcggccagg
     1261 cagctcacgc cggtctttct gtctcggttc gatacagtgc ccgaaccgat gatggtacct
     1321 gcactgagcg ccctggtttt ggcggcatgg gcgattaact cggccatact gaagttcatg
     1381 tcgcaaccgg cgttgggact gccgattagc tgaccgttga gataggtaag caaaggcagg
     1441 tgcagttttg cgtctttcca ggcttggccc agttcgtccg gggtgactgc caccggtgca
     1501 aaagcgctgg aaggtttgcc atgcacaaaa ccgaatccct ttgccaactc cgaggggata
     1561 agctctctca aagacatatc gttgacaatc atgaccagtt tgatgtgtgc ggctgccgct
     1621 tcggcactta tacccatggg tacgtcgtcg acgattaccg ctacttctgc ttccatgtcg
     1681 gcgccgtagg cttccgaagc aagaggaaag gcttccagcg gcgcgaggaa ggcatcgctg
     1741 ccgccctggt acatgagtgg atcggtcagg aatgattcag gcatttcggc gccgcgggct
     1801 tttctgacca gttccacgtg gttgaggtaa gcactgccgt cgagccattg gtaggcacgc
     1861 ggcagagggg aaaggcagtt gcccggttca aaatcaatag caccggcggc ttgaccggct
     1921 tcgaggcgct cgaactcttg ctttgcctgt ttttccaatt gctggaaatt atcaagaaaa
     1981 tattgcagag tggctgcaat ttgcggaatg ggtagagctt tggtcatggt ccggtttacg
     2041 agcacaagca tgccgtctct ggtgccgttt ttcacggttg caagtttcat tcatgatcct
     2101 ccgattttga ttctgtttat gctaacacgc cgccctgaaa gccgttcctt accggggata
     2161 ttccggctca ggtcgcgttt ggtgaatgaa agcagatgct tttgctttcg gaagggttag
     2221 gcagtcgcgc tgctccagat tttgccttta tggaatttgc cataactttc attggctcat
     2281 gggtgttctg ctcggcgcaa gtcacggcgc tgatgaaagg gcaatactaa tgtagcttca
     2341 atctgagagt tcgctttgcc tgaaacctgg ttgaggtaaa aatgtctata accacatact
     2401 tatcaagagg tagtgcttgt atgcaagaaa aatgttgccg cttgcgggcc gctttgttgg
     2461 gcgattttga gactcttgct gcttgaccac tgctctgtgg cttatgctga ctgcctcggt
     2521 caggccattg aggcttgcca tcacgggagt tacagtaaag acgtaaaggc cgctatggtg
     2581 cgcagggtgt gggacggtgc gcgattgcca agagtgcttg caaccaggta cgcagtcctg
     2641 gcctgtattt cggagacagg tgcaatttcg gcttcgcaat tgtctgcata actaaatttc
     2701 tctcaagctt tacctcaagc aaaaagtgtt ggtaccaccg gaagtgccgg tcttgccggt
     2761 agatggtgta agagccgaaa gttatgccga ttgtctgtgt ttcttcggga gagaatagtg
     2821 ttttcaggaa atttgcaaat ggccatgagt atttacgaag cttggttatt ccccgtcttt
     2881 ctacattgaa aaacctgacc taagctcttt tgcaacacaa ataatcgtcg ttccgtgcac
     2941 cagccgtgca ctgagcctgt tgacggcgtt ttagaagcgc gtatttattg acggcatgcc
     3001 g
//
LOCUS       DAOQNZ010000365         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17259_length_3001_cov_3.633401, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000365 DAOQNZ010000000
VERSION     DAOQNZ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17259_length_3001_cov_3.633401"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PKW73_07655"
     CDS             <1..1104
                     /locus_tag="PKW73_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNA73197.1"
                     /translation="GGLNITHSEPYGSFVGRYGDRRKEMQSLLDRLPPDALREWVHGL
                     GIKTFVGSSGRVFPSEMKAAPLLRAWLQRLRHCENGSVRLHVSTPWLGFNDDGSLRMS
                     NSAGEFSLRPAATILALGGASWPQLGSTGAWVPWLAERGVKIAPWQSANCGFDVAWSA
                     HLREKFAGAPLKAVALTFVDVNGRKETKEGELLVSDYGIEGSLIYTFSRRLRDYINVH
                     GQATFTLDLLPSRSAERVKADLARPRGSRSLSRHLQSTLGISGLKAALLYEVLSRDEM
                     NDSVALARAVKALPIKLLSARPLAESISTAGGVRFANLDENLMLKALPGIFVAGEMLD
                     WEAPTGGYLLSGCFASGYCAGQGVKRWLESAGH"
     gene            1273..2910
                     /locus_tag="PKW73_07660"
     CDS             1273..2910
                     /locus_tag="PKW73_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73198.1"
                     /translation="MSVLLAASVLIGVAVPSALGQAGAASPELNSMWSRLNQIYVQSN
                     MLNTDDASEVEIMLKAAIEKSGVLYGADSEEVLQATLELARLYRMQSRYGELKLLLPR
                     AVAIYEKLPQAARRPFSGSMLEAACDLVNRSKVKEANLLLAPVLENLKADRAQLDDQA
                     FLSRLTSLAQSLKSRREFAACEPIYRALLFAIENKQPVNQVTLADARTQLAEVLKETG
                     NTSTAMELATLALAEQEKAGTAGLSSMVPSLSLLTYLELASGKTESAARSAVKLDSAL
                     AKAVVCDRGGVFRSLLELCKQFAQVNDSQSVALLFGRSLAYADQYSYSYYDNSLREIS
                     RYLIASGNSSRAEALYGQLLQWVERPGSNVDAGYLPNVISMQARFFIQSGQFDKLDKV
                     MTRLAAFDSEHQTRNLRNLQSEVEQSGSLSAREKLYSLLIAGEASSARDRLQLVNYRN
                     KLAQMYVQSGRTGEAKSQWFEITTVLEKNVPEDCRDIGAEVRTVLDAYITEKRYQEAQ
                     QLVNNMVTYGFNRDIQVSVANGFTALATHHEQLGNLD"
BASE COUNT          616 a          717 c          893 g          775 t
ORIGIN      
        1 gggggcttga atatcaccca tagcgaacct tatggttcct ttgtcggtcg ctacggcgac
       61 aggcgcaaag aaatgcagtc tctgcttgat cggctgccgc ctgatgcttt gcgagagtgg
      121 gtgcacggac tgggcatcaa gacttttgtg ggtagttccg gtcgggtttt tccctcagag
      181 atgaaagcgg caccgctttt gcgggcctgg ttgcagcgcc tgcgtcattg tgaaaatggt
      241 tcggtgcgtt tgcatgtgag cacaccctgg ctcggcttta atgacgatgg ttctttgcgc
      301 atgtctaatt ccgcaggcga attctccctc agaccggcgg ccaccatttt ggccctgggc
      361 ggtgccagtt ggccccagct gggttcgacc ggcgcctggg taccctggct agcggagcgg
      421 ggggtgaaga ttgcaccctg gcagagtgct aactgcggtt ttgatgtggc ctggagcgca
      481 catttgcgcg agaaatttgc cggtgcgccg ctcaaggcgg tggctctcac ttttgttgat
      541 gtgaatggtc gcaaggagac gaaagaaggc gagctgcttg tcagtgatta tggcattgaa
      601 ggcagtctca tttatacttt ctccaggcgg ctgcgcgatt atataaatgt gcacgggcag
      661 gctactttta ctcttgatct cttgccctcc cgcagtgccg agcgtgttaa ggccgatctg
      721 gctcgcccgc gcggctcgcg ctctctttct cgccatttgc aaagcacgct tggtataagc
      781 ggtctgaagg cggcgctgct ctatgaagtg ttgagccggg atgagatgaa tgatagtgtt
      841 gctctggcta gagcggtaaa agctttgcct ataaagctgc tttcggccag accgctggct
      901 gagtccatta gcaccgccgg tggtgtaaga ttcgctaatc tggatgaaaa tctcatgctc
      961 aaagctcttc ccggcatctt tgtggccggt gagatgcttg attgggaagc tcccactggt
     1021 ggatatctgc tttcgggctg ctttgcctcc ggctattgtg ccgggcaggg ggtgaagcgg
     1081 tggctggaga gcgcagggca ttagtgcccc ggccgggcgg gcaaggggat agagaatttt
     1141 ccagggaaaa cccagttatg ctctaactgt ggctgtggta aaactggtga tattgatact
     1201 ttaatgtaga tgcaaaatta aactgtctat gtgggtgaat ttatgcctcg aagcagcaga
     1261 agcacaaggt gtttgagcgt tttactggca gcatcggtct tgattggggt tgccgtgcct
     1321 tccgccctgg ggcaggccgg tgccgcctct cctgagctta attccatgtg gtcgcggctc
     1381 aatcagatat atgtgcagtc gaacatgctc aatactgatg acgcgtccga ggttgagatc
     1441 atgctcaaag ccgccattga gaaaagtggt gtcttatatg gtgctgattc cgaagaggtg
     1501 ctgcaagcca cccttgagct tgccaggctt tatcgtatgc agtctcgcta tggtgaattg
     1561 aagctactgc tcccaagagc ggtagccatt tacgagaagt tgccccaggc ggcccggcgg
     1621 ccgttttcag gctctatgct tgaggcggct tgcgatcttg tcaatcgcag caaggtgaag
     1681 gaagccaatt tattgcttgc gccggtgctt gagaatctca aagcggacag ggctcagctt
     1741 gatgatcaag ccttcttgag caggctgact tcccttgccc agagccttaa gagccgtcgc
     1801 gaattcgccg cctgcgagcc tatctaccgc gctctcttgt tcgccattga aaataagcag
     1861 ccggtcaacc aggtcactct tgccgatgca cgtacacagc tagccgaggt tttgaaagag
     1921 actggcaata cctctaccgc tatggagctg gctacgctgg ctcttgctga acaggagaag
     1981 gcgggaacgg caggactgag tagtatggtg ccgtcgctca gtttgttgac ctacttagag
     2041 ctggccagcg gtaagacaga gtcggctgcc aggtctgcag tcaagcttga tagcgctctt
     2101 gccaaggctg tggtgtgtga tcggggcggt gtttttcgtt ctttacttga gctttgcaaa
     2161 cagttcgccc aggtcaatga tagccaatct gtggcattgc tatttgggcg gtcgctcgct
     2221 tatgccgatc agtatagcta ttcatattac gataatagct tacgtgagat ttccaggtat
     2281 ttaatcgcct ccggcaatag ttccagggcg gaagctctct atgggcagtt attgcagtgg
     2341 gtagagcgcc cggggtccaa cgttgatgcc ggttatttgc ctaacgttat ttccatgcag
     2401 gctcgctttt tcatacagag cgggcagttt gacaagctcg ataaggtgat gaccagactg
     2461 gctgcttttg atagtgagca ccagacgcgc aatttgcgaa atttgcaatc tgaagttgag
     2521 cagtcgggaa gtttgtctgc acgtgagaag ttatactcgc tcttgattgc cggcgaggcc
     2581 tcaagtgccc gagatcgtct tcagctggta aattatcgca acaagctggc gcagatgtat
     2641 gtgcagagcg gccgcactgg tgaggctaag agtcagtggt ttgagattac caccgtgctt
     2701 gagaagaatg tgccggaaga ctgccgtgat atcggcgccg aggtgcgcac cgtgcttgat
     2761 gcttatataa ccgagaagcg ctatcaagaa gctcagcagc tagtaaataa catggttacc
     2821 tatggtttta accgtgatat tcaggtgagc gtggccaatg gctttacggc tctggcaacc
     2881 catcacgagc agttgggaaa tctggattaa gcactgtccc tggtgcagcg ctccctgttg
     2941 gtggctcaga agtatggcgg caaggacacc tataattttg ccgatgccct tggtcagtat
     3001 g
//
LOCUS       DAOQNZ010000366         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17286_length_2999_cov_4.204823, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000366 DAOQNZ010000000
VERSION     DAOQNZ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17286_length_2999_cov_4.204823"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKW73_07665"
     CDS             complement(<1..753)
                     /locus_tag="PKW73_07665"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73199.1"
                     /translation="MPALVLAGEEEFLLYRKLEELKEKLLDPAFASFNYQRLDGAVSG
                     SDVVDLAASIPFGPGNKVIIFDRCDWFAKKRAGKSDDKPKTGKAGKASGKDSVDEDKL
                     AEALAGLHPNTYLIFVATQNFDSTLRLSKLVAQHAKVEKFDKFKHWAGGEASTELKNF
                     VQKEVHHFGATIDEDAAYYLIDGLEANLRHIHKEIEKAATYILPQTHITLDVVKRLSP
                     HNSQIFAVTEYWLAGKHEQTLNSLRELLSKQNA"
     gene            complement(807..1451)
                     /locus_tag="PKW73_07670"
     CDS             complement(807..1451)
                     /locus_tag="PKW73_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HNA73200.1"
                     /translation="MKGESITLIKEEVLTLGRLVETTVYEVTRMLKGDKKADLALVER
                     QEEEINRLCLEIEEKCVDLLIAREVTHGEREIRALVSIPIIAAKLERLADHANRVARF
                     ATWAVEDQIEIPEELPKMAASVYHMVQELLLCFLTDSPSKAKEILASDSNVDYLHDLL
                     SKRLLSDLGEQESARAQMKAQFLFCARFLERMGDACTSIAKRVYFLSTGERISH"
     gene            complement(1480..2199)
                     /locus_tag="PKW73_07675"
     CDS             complement(1480..2199)
                     /locus_tag="PKW73_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA73201.1"
                     /translation="MAVKTRIMVVDDDETIIDTLTFNFKRAGYEVISFNRAMEALAGF
                     NDAKPDLVMLDWMMPDMKGPDLCALLREQAPEMPILMLTGRSTPNDIAHGLSSGADDY
                     LVKPFSLVELMARIEALLRRSGKGRKFKPLSSGPIVLGTLTLDDEARRVTYEGRNIDL
                     SPREFNLLRVLMLNVGKVLTTESLLDKVWGTDFDGDVKTVAVHMRWLRQKLESDPKCP
                     KLLETVHRSGYRLNNPADMAK"
     gene            complement(2210..>2999)
                     /locus_tag="PKW73_07680"
     CDS             complement(2210..>2999)
                     /locus_tag="PKW73_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA73202.1"
                     /translation="LLLTCRSASPIAYFFLRQLVSNMVPDKHNLPSSRDDHSNWIAEV
                     SHELRLPLANIKLLVETLLSGAVDEPATARRMLARADEEVSRLQNLVSDLLSHEKLSK
                     SGSTTGTMVLVLHKVEAALETTLPLARAKNITVRVEVEEGTQFCVDGDQLHQVLVNLL
                     ENAIKFTGENGLVTVRASRTPLKLEVEDTGIGIAESEIPKIFQRFYRVDRSKTRGSTG
                     LGLTIVKHIVDLHGAKISVNSREGEGTCFIIEFPQTLSESGIGG"
BASE COUNT          711 a          798 c          726 g          764 t
ORIGIN      
        1 ggcattctgt tttgaaagca gttctctcag actgttgagc gtctgctcat gcttgccggc
       61 cagccagtat tccgtaacgg cgaaaatctg agaattatgg gggctcaagc gtttgacaac
      121 atcgagagta atatgagtct ggggaagaat ataggttgcc gctttttcaa tttccttgtg
      181 aatatggcgc aaattggctt ccagtccgtc tatcaaatag taggcagcat cttcatctat
      241 ggtggcgcca aaatgatgca cttccttctg cacaaaattt ttcagttccg tactggcctc
      301 gcctccggcc cagtgcttga atttgtcaaa cttttctact ttggcatgct gggcaaccag
      361 ctttgaaagt cgcaaggtag agtcgaagtt ttgagttgcc acgaatatca aataggtatt
      421 cggatgcaag ccggccagag cttcggccaa cttatcctca tcgacggaat ctttaccgga
      481 agcttttccg gctttgccgg tcttgggttt gtcgtcggat tttccggctc gcttcttggc
      541 gaaccaatcg cagcgatcaa aaataattac cttattgccc ggtccaaaag gaatggaggc
      601 agccaggtct acgacatcac tgccgctcac ggctccatcc agcctttgat agttgaagct
      661 ggcaaaagcc gggtcgagga gtttttcctt caactcctcg agctttctat atagaagaaa
      721 ttcttcctct ccagctaata ctaatgccgg caatgaaacc tcaactgacc cttaaaaatt
      781 gaaccttctg tgacttgcag agactttcag tgactaattc tctcaccagt ggagagaaaa
      841 tatacgcgct tggcaatcga agtgcaggcg tcgcccattc tctcaagaaa gcgggcacag
      901 aagagaaatt gcgctttcat ctgggcccgc gcactctcct gttcacctag atccgaaagc
      961 agacgcttgg ataagagatc atgcaagtaa tcgacgttgc tgtccgaagc tagaatttcc
     1021 ttagccttgg agggcgagtc ggtaaggaag caaagcaaga gttcttgcac catgtggtag
     1081 acactggctg ccatcttcgg cagttcttcc ggaatttcaa tttgatcttc caccgcccat
     1141 gtggcgaagc gagcaacgcg gttggcatga tcagccagtc gctccaactt ggcggcaatt
     1201 ataggaatgg aaaccagggc tcgaatctct ctttccccgt gggtgacctc cctggcaatc
     1261 agcaagtcaa cacatttctc ttcgatttcc agacagagtc ggttaatttc ctcctcctgc
     1321 ctctccacca gagccaggtc ggccttcttg tctcctttga gcatgcgtgt cacttcgtag
     1381 acggtggttt ccaccaaccg tcccaaggtg agaacctctt cttttattag agttatcgat
     1441 tcacccttca ctttacttac tcccgagatc tcatctctat catttggcca tgtcagccgg
     1501 attattcaga cggtaaccgg agcggtgcac ggtctcaagt aacttcggac acttgggatc
     1561 gctctccagt ttctgcctga gccaacgcat atgtacggcg acggttttta catcgccatc
     1621 gaaatcggtg ccccagactt tatcaagtag agactcggtg gtcagcactt tgcctacgtt
     1681 gagcatcaag acccgcaaaa ggttgaactc tctgggggac aaatcgatat ttcgaccttc
     1741 gtaggttacc cgccgcgcct catcatccag agtcaaagtg ccaagcacga tgggtccgct
     1801 gctcaagggt ttgaacttgc gccctttgcc gctgcgtcgc aacaaggctt caatacgggc
     1861 catcaattcc accagggaaa aaggcttgac cagataatcg tcggcgccgg aggagagacc
     1921 atgggcaata tcattgggag tggaacggcc ggtgagcatc aagatcggca tctcaggtgc
     1981 ctgctccctc aaaagggcgc agagatccgg tcccttcata tcgggcatca tccaatcaag
     2041 catgaccaga tccggcttgg catcattgaa tccggccagg gcctccatgg cccggttgaa
     2101 ggaaatcacc tcatagccgg ctcgcttgaa gttgaaggtg agggtatcga tgatggtttc
     2161 atcatcgtct acgaccataa tgcgagtctt cactgccatt ctcttctatt cagcccccga
     2221 tgcctgactc tgacagagtt tgaggaaatt cgatgatgaa gcaggtgccc tctccctctc
     2281 tggaattaac tgaaattttg gcgccgtgta agtcgacaat gtgtttgacg atagtcaggc
     2341 caagtccggt ggagccccgg gtcttgctcc tatcaacgcg gtagaagcgc tgaaatattt
     2401 taggaatctc gctttcagca atgccgatgc cggtgtcctc cacttccagt ttaagaggcg
     2461 tgcgcgaagc gcgcacagtc accagcccgt tctcaccagt gaacttgatg gcattttcca
     2521 ggagattgac cagtacctgg tgcaattgat ctccgtcaac gcaaaactgg gtaccctctt
     2581 ctacttccac ccgtaccgta atattcttag ccctggccag gggcagagta gtctccagcg
     2641 ccgcttcaac tttatgcaac accagaacca tggtgccggt tgtggaaccg ctcttgctca
     2701 gtttttcatg agacagcagg tcggagacta aattctgcag acgacttact tcttcatcgg
     2761 cgcgagccag catgcgcctg gccgtagccg gctcgtcaac cgcgccactt agaagcgttt
     2821 caacaagcag cttgatatta gccagcggca gtctcaactc atgagaaact tcggcaatcc
     2881 aatttgaatg atcatccctg gagcttggca aattgtgctt gtcgggcacc atatttgata
     2941 ctaattgcct gagaaagaaa taagcgatcg gcgatgccga tctgcaggta agcaaaagt
//
LOCUS       DAOQNZ010000367         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17302_length_2998_cov_2.831125, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000367 DAOQNZ010000000
VERSION     DAOQNZ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17302_length_2998_cov_2.831125"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..994)
                     /locus_tag="PKW73_07685"
     CDS             complement(50..994)
                     /locus_tag="PKW73_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNA73203.1"
                     /translation="MSMGRLSLGKREHLRASGTVILVAAFMLGSPALAQGQAEPLLQK
                     LYMPQEQTEKAAPTSLQPRPITSASTARRFKTIKIAGFCSFVLPGDKLFMGGNPVLGL
                     EADARLSEVLPVLENLKNHPITINVHSDNMGFSHFNDRLTEKRGELLRDWFLKHGHFD
                     PEGITVVGHGGSRPLEPNQLPGGKDNPAGRAANRRVEIVVETLAGFVSSKEKEARRLA
                     AAAAEEESSGSDKDKSAKQPEVDVSKLPPELQELHQIMNPDKTAAGEAEPEPEPPVSD
                     HTADGRRKIPELSAAEKEKLKKETEWARKEFGLFQPLQ"
     gene            1096..1899
                     /locus_tag="PKW73_07690"
     CDS             1096..1899
                     /locus_tag="PKW73_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73204.1"
                     /translation="MKSGSFTTDQFLASLSGRFLPALMLGLVLSGAALSAPGPTPARS
                     NSQEKVKLQVIRIESSANGPYKILVNKDYLRVDSSSFGLTIYAARPDWQVTAVRAKQR
                     ELASASYQQWQKTIVPSFTMAGICQELKAPTASIKGKDGSTRNVFMHKGKSETFFQTR
                     EERTEIKGVEIDTVPVDTDPHALAIINHFLNLPSLPGVPVALNVLYENGKKGWQIRRI
                     GATSTELSRSEILPPKEGFQNTGRISKEFLARSAMTTLDGVSELLSEPK"
BASE COUNT          707 a          826 c          772 g          693 t
ORIGIN      
        1 tcgcctggaa atttaattaa ggaccagtct cagtaaccca ctccaacagc tactgcaaag
       61 gctgaaagag accgaactct tttctggccc attccgtttc tttcttcagc ttttcttttt
      121 cagcggcgga aagctcggga attttgcgcc ggccgtcggc cgtatgatcg ctcacgggcg
      181 gctccggctc cggttcagct tccccggcag cagtcttgtc gggattcatg atctgatgca
      241 attcctgcaa ctcagggggc agcttgctta catctacttc gggctgcttg gcagacttgt
      301 ctttgtcgct gccgcttgac tcttcttcgg cagcggccgc agccaatcgc cgggcttcct
      361 tctctttgct ggagacgaat ccggccaggg tctcaaccac aatctccacc cgcctgttgg
      421 cggctcggcc ggcgggatta tccttaccgc cgggcaattg attgggctcc agcggtcgac
      481 tgccgccgtg acccacaacc gttatgcctt ccggatcaaa atgcccgtgt ttgaggaacc
      541 agtcgcgcaa aagttcgcct cgcttctccg tcaatctgtc gttgaagtga ctgaagccca
      601 tattgtccga gtggacgttg atggtgatcg gatggttctt gaggttttcc aacaccggca
      661 agacttcgga aagcctggca tcggcttcta aacccaagac cgggttaccg cccatgaaga
      721 gcttgtcgcc cggcagaaca aaactgcaaa aaccggctat cttaatggtt ttgaagcgcc
      781 gcgccgtact ggcggaagtg attggtcggg gctgaagact ggtcggagcg gctttttcag
      841 tttgctcctg gggcatatag agcttttgta aaagcggctc agcctggccc tgggccaaag
      901 ccgggctgcc gagcatgaac gccgccacta aaataacagt gcctgaagcc ctcaaatgtt
      961 cgcgcttacc gagagaaaga cgacccatag acataaagcg acctgtttca ctgcccgaaa
     1021 catcataagg cctgcctgaa gtttctacac tagctgggca taatgtattt acggcgactt
     1081 ttagtgcaag ttctcatgaa atcaggctct tttaccacag accagtttct tgccagtctg
     1141 agcggcaggt tcctgccggc tctgatgcta ggtctggtct tgagtggcgc ggccttgagc
     1201 gcaccaggcc ctaccccagc cagaagtaac agccaggaaa aggtcaaatt gcaggtgatt
     1261 cgcatcgaat caagcgccaa tggtccctat aaaattctgg tcaataaaga ctacctgcgt
     1321 gtggactcca gttccttcgg actgaccatt tatgccgccc gacccgactg gcaggtgacg
     1381 gccgtccgtg ccaagcaaag ggagctggcc agtgccagct accaacagtg gcagaagacc
     1441 attgtgcctt ctttcacaat ggccggcatc tgccaggaac taaaagcacc gacggcatcc
     1501 atcaagggca aagacggcag caccaggaac gtgttcatgc acaagggcaa atcggaaaca
     1561 ttctttcaaa ctcgcgaaga aagaactgaa atcaaaggcg tagagataga caccgtgccg
     1621 gtcgataccg atccccatgc cctggcaatc atcaatcatt ttctcaattt gcctagcctg
     1681 ccgggtgtgc cggtggctct caacgtgctc tacgaaaacg gcaaaaaggg ctggcaaatc
     1741 agacgcatcg gcgctacctc gacggagctg agccggagtg agattctgcc gccgaaagaa
     1801 ggtttccaaa acacaggccg aatatccaag gaatttctag cccgcagcgc catgactaca
     1861 ctggatggcg tctcggaatt gctctcggaa ccgaagtaac gttttgtgct gacgccccgt
     1921 acatctagcg ctattgcacc tctttcagga gatggcgcta gcgacgctag ccaccagccc
     1981 aaatttataa tcttccattt gcttcagaat gaatcttcgc gctaaccagg ccagatcttc
     2041 atgacatcca ccagaaggct tataccgtca ttgttgcgag ggatttacaa gaaccccatt
     2101 taaaaatcag aaacacttct ctattggagc caaagattct ttgagctagc ttcgaatagt
     2161 cctctaattc acttcacccc gagaatctct caagactcca tcttcagcca tatccgtcca
     2221 ccccccctgc ttacacctgc ggtgtcggta tgaggctgtg tacaggagca aggcgaagac
     2281 tttgaggcgg aaatcgaaag attacatctc ggtcgaaggt cgaagagaga ttctccccga
     2341 ggagatgctg aggaaaacgc tgcactccaa ctcaaggaag tgcttgtgat gggtccaaac
     2401 cactcggagg catccatgtg ctggccttaa ataacacctc gcagctgccg gtagtggtct
     2461 gagtgtcacc tcagacgtag aaccggcaaa gcctgattta gaccagtgac gaatttcggg
     2521 tcggtcaggt cgggcctggt tcgggtccgg gtgaagccaa tatcacaggt ttcgccgcag
     2581 gctgcagatg agacctaccc tcggaaaggt ttcagcttca gtcgaaccct actgctttga
     2641 ctcgtcgaac aaagcaaggc cattattccg gagggtctag ggtcctttct ctgccggaat
     2701 tatggataac gacgcgtaac cgaaagccac ccggacgggg cttttatggg gcgtactaaa
     2761 aaccgtcaag ctcctgagag gcggaatacc gtgaggtcgg attctgcctg gagcggggac
     2821 ggcaattctc cattttttta tatcggggaa ttgccgttat gtttattttt attatgaagt
     2881 cgctaagctc aatcctcgga gcaggagcgg cacgaaggtg cgcttgtccg gcgtactttc
     2941 gcgagaggca caacctcaaa attctatttt tggaattttg aaaccgttgc ctcgcccc
//
LOCUS       DAOQNZ010000368         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17370_length_2990_cov_3.769336, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000368 DAOQNZ010000000
VERSION     DAOQNZ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17370_length_2990_cov_3.769336"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2615)
                     /locus_tag="PKW73_07695"
     CDS             complement(<1..2615)
                     /locus_tag="PKW73_07695"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNA73205.1"
                     /translation="MVNTVDKKSENLPDAPRKISESKGVTEFRLANNLRVLISENHSA
                     PVSTLLVVYRVGSRNEGVGYTGSTHFLEHMLFKGTEEHNAEKGNGLDDLLTQIGAYWN
                     ATTWFDRTCYYEVVPSEFFELCVKLEADRMRNLRLRQEDHDSEMSVVRNELERGENQP
                     EEAMEKELYAIAFREHPYHHPTIGWRSDVEGVPMDRLRQFYNTFYWPDNATVIALGDF
                     ATDEALNCINKYFGAISAAPHPIPTVYTVEPPQEGERRFEISRAGDLPRVWVGYHVPE
                     AAHKDNYAVSAVRQILGSTYERSSRLYKSLIDTSLAADVFARHDDLRDPGLFIVGAVV
                     NPGVDAREVEAILLAEMEKFAAAPVTPDELSRIKSANRKGTILAKADPSSMAFMLGEA
                     ESRADYNWLLDYDEHFDAVSAEDIQRVAGLYFKRSNRTVGYFYPREGEEFEDASLEGE
                     EEELQEMEEAPEEETSVQAQPLYKESEMKTLMALNSSPVTIRYEKAKKSDYLTRVEKV
                     VLKNGLTVLLMKNPGTESVGVTVNLKAGRYFTSRDEGSVSELVGDLLSRGSRSYDKIK
                     IAENLEEMGIPGGLEFYVDNYRVGLGTHLVAGDLSRYLDMLVDILRYPSLSEEELTKI
                     KIEWNSRFVESKMSTKSMAWNKLRQSLYPADHVFYDKDFDKQIEELAAVTRADLVAAH
                     GRLYSPSATIITIVGDIEPKEALKLVEQRFGDWQDEKLTLKPAERQIEIPLVSPPEKA
                     FTVEVFLPEKPSLDIVMAHPCAVRRDSPDFYAARIANAALGQDTITSRLGQVVRDRAG
                     LTYGIYSGFSDTAYGGAPWTVSLTTNPKNVARALQLTRATLKDYIDRGIDKNDLEKES
                     GRALGSFKVGLSSSLG"
BASE COUNT          633 a          788 c          809 g          760 t
ORIGIN      
        1 ccaagagagg aagagagtcc gactttgaaa gaaccaaggg cgcgaccgct ttctttttcc
       61 agatcattct tatcgatacc tctatctata tagtctttca aggttgctct ggttagttgc
      121 aaggcgcggg ctacgttctt cggattggtg gttagcgata ccgtccacgg tgcaccgccg
      181 taggccgtat cggagaagcc tgaatagatg ccgtaggtaa gaccggccct gtctctcact
      241 acttgtccga ggcgggaggt gatggtgtcc tggccgagcg ccgcattggc aatgcgtgcc
      301 gcatagaaat ccgggctgtc gcgccgcact gcacaggggt gggccatgac tatatccagg
      361 ctgggtttct caggcaggaa cacttccact gtgaaagctt tttccggtgg gcttaccaat
      421 ggaatttcaa tctggcgttc agccggcttg agggtgagct tctcatcctg ccagtcaccg
      481 aaacgttgtt ccaccagttt gagggcctct ttgggttcaa tgtcaccgac gatggtgatt
      541 atggtggcac tgggactgta aagacgccca tgggctgcca ccagatctgc tctggtaacg
      601 gcggctaatt cttctatttg tttgtcgaag tctttatcgt aaaaaacatg gtcggcggga
      661 taaagggact gtctgagctt attccaggcc atcgacttgg tggacatctt tgattccacg
      721 aagcgggagt tccactcaat tttgatcttg gtcagctctt cttcggaaag tgacggatag
      781 cgcaagatgt ccacgagcat atcgagatag cggctcaagt caccggccac cagatgagta
      841 cctagaccaa cacggtagtt gtccacataa aactccagac cgcctggaat acccatctct
      901 tcgagatttt cagcaatttt gatcttgtcg taggagcgcg agccccggga gagcaaatct
      961 ccaactagtt ccgagaccga cccttcatcc cggctggtga agtaacggcc ggctttcaga
     1021 ttgactgtaa cgcccactga ctccgtaccg ggatttttca tgagcagcac ggtgagaccg
     1081 tttttcagta ctaccttctc aaccctggtg aggtaatcgc tctttttagc cttttcatat
     1141 cggatggtga cggggctgct attgagggcc atgagagtct tcatttcaga ctctttgtaa
     1201 agcggttgag cttgtacgga agtctcttcc tccggtgcct cctccatttc ttgcagttct
     1261 tcctcctctc cttccaggct tgcgtcttcg aactcttccc cttctctggg atagaagtag
     1321 ccgaccgtgc gattggaacg tttgaagtag agaccggcca ccctctgtat atcttcggca
     1381 ctgacggcgt caaagtgctc gtcataatcc agaagccagt tgtagtcggc tctcgattct
     1441 gcctcgccga gcatgaaagc catactggaa ggatcggcct tggccaggat ggtacctttg
     1501 cgattggcgc ttttgatgcg gctcagttcg tcgggggtga ccggagcagc ggcaaatttt
     1561 tccatttcgg caagcaagat tgcctctact tctctggcat ccacaccggg attgaccacg
     1621 gcgccgacga taaagagacc gggatctctc aagtcatcgt ggcgggcaaa gacatcggcg
     1681 gccagggagg tgtcgatcag gctcttatag aggcggctac tgcgctcgta ggtgctgccc
     1741 aaaatctgcc gtacggctga gacggcataa ttgtccttat gggcagcttc cgggacatgg
     1801 tagcccaccc agacccgggg taaatcgccg gctcgggaga tttcgaaacg gcgctctcct
     1861 tcctggggcg gttccaccgt gtagacagtg ggaatgggat ggggagcggc actgatcgca
     1921 ccaaaatatt tgtttatgca gttcagtgct tcatcggtag caaaatcacc cagggcaatg
     1981 accgtggcat tgtccggcca gtaaaaggtg ttgtagaact gcctgagtct atccatgggc
     2041 acgccttcca cgtcggagcg ccagccaata gtgggatgat gataagggtg ctcgcggaaa
     2101 gcgatggcat agagttcctt ctccatagcc tcttccggct gattttcacc tctttccagt
     2161 tcattgcgca ccacgctcat ttcactgtca tggtcttcct ggcgcagccg aagattgcgc
     2221 atgcggtctg cttccagctt tacgcaaagt tcaaaaaatt cgcttggcac cacttcgtag
     2281 tagcaggtgc ggtcgaacca ggtggtggca ttccaatagg caccgatttg tgtgaggagg
     2341 tcgtcaaggc cattgccctt ctcggcattg tgttcttccg tacccttgaa gagcatgtgt
     2401 tcaagaaagt gagtggagcc ggtgtagccc accccttcat tgcggctacc gacccgataa
     2461 accaccagca aggtagaaac cggcgcactg tgattttccg agatgagcac cctcaaatta
     2521 tttgccagcc tgaactcggt aacacctttg ctctcggaaa tttttctggg ggcatcaggt
     2581 aggttttcag actttttgtc cacggtgttt accactctcg gttcctcttt tatgcgcatc
     2641 gggccttaag tgcttaaaga taactcaaat ccctcccatc cgccctaaat tgaaagtggc
     2701 aatccactgc taaaagcaca ttgcgcgcta ctccggcgta tgacaaccaa ttcatgccgg
     2761 tgcgtagcaa taaaaaggtt gttaggggta aaataggggc ttgtgaagag gtcccccaag
     2821 tgttcccccc taaggcttac tgcgcctgtc ttttgcttgt cttgacctgg ctctcggcag
     2881 ccggtgtcat ggctctggat ggcgctgcct gggccaggct cacccgtgag ggctcgaaag
     2941 cctatgagag cggcaatttt ggtgaagcag agcggctgtt caagcaggcg
//
LOCUS       DAOQNZ010000369         2989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17379_length_2989_cov_4.091002, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000369 DAOQNZ010000000
VERSION     DAOQNZ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17379_length_2989_cov_4.091002"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PKW73_07700"
     CDS             <1..828
                     /locus_tag="PKW73_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNA73206.1"
                     /translation="TGSPSKKRQAKAEADKIAGKINELYRSFMQSYVRSINESTFKVT
                     IGGVRIGNAKYSRLAQINLKSRIITFSRYAIENVPERGRRYLVLHELAHVLEASHNKH
                     FWNLVEFHEPQYKEIGLALDRAFKKNVQKHERLEKQNAEQTLENAVRDLNLAAAKDLI
                     WTPDHGFIESGDRNFGLHRDQLRLFTSSADGRHIDEGFNEYLALKNEDDDELEPNLIL
                     DPHERALAEWENFMKKDPQDDQDSYEIGSESGQGGCFENSDDYFDDDYLYGTMSGGD"
     gene            1088..2368
                     /gene="fabF"
                     /locus_tag="PKW73_07705"
     CDS             1088..2368
                     /gene="fabF"
                     /locus_tag="PKW73_07705"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNA73207.1"
                     /translation="MNAIDKKERRVVVTGIGMISPVGQSAKECWEALLSGTSGVGSLT
                     LFDPAAHGIETRIAAEVKNFNIQDFYPDKRKAGNFLKEMDRVTLFAMAAAKLAVDDAA
                     LKIDEGNAERVATFIGTGVGGLTTTLQDYQRLLEGGPRKVGVRSVIKLMPNAPSGQVA
                     IEFGAKGRAKGDATACASGLDSLLDAFMYIQDNRADVVIAGGAEAAINAIGVTSFGNM
                     MALSKRNDEPTKASRPFSADRDGFVIGEGAAVLILESLEHARARNAKIYAEIKGGAAS
                     CDAHHIVAPNQSGEGAARAIAEALKDAGLEPTEIDYINAHGTSTPLNDACETKAIKTV
                     FGDYAHKLAISSTKSMIGHLLGGAGAMGAAVTALSIKDSKVHPTINYDNPDPECDLDY
                     VPNVSRQLNVRNALVEALGFGGHNTVLVMGQLKD"
     gene            complement(2377..>2989)
                     /locus_tag="PKW73_07710"
     CDS             complement(2377..>2989)
                     /locus_tag="PKW73_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73208.1"
                     /translation="QSALPSAVDAVPIARAVSFPVTFTYNGDGRLDSRLDKPASSQFV
                     IPSILNATDARLLNLSFSGRRDPYSSNSTALGSGQGIVSASGTSKLVNDGHLYNSLIA
                     SEAEGGNGHDARKLAGMEEAEDDVDKAGSGVVASANNGNQDDDDEDGQQSAAPVQVSS
                     GSNVPPLPASVLTGNAVVADAGKAKEAEKEKIPGPPRKKTITA"
BASE COUNT          794 a          779 c          785 g          631 t
ORIGIN      
        1 accggctcgc cgtcaaagaa aaggcaagcg aaggcggaag cggacaagat cgcaggcaag
       61 ataaatgaat tatatcgatc gtttatgcaa tcctatgtca gaagcataaa cgagagcact
      121 ttcaaagtaa ctatcggcgg cgtcagaatc ggcaatgcaa aatattccag actggcccag
      181 atcaatctaa aaagtcgcat catcactttc tcgcgctacg ccatcgaaaa tgtgccggag
      241 cgcggccggc gttatctagt gctgcacgag ctggcccatg tgttggaagc ctctcacaat
      301 aagcatttct ggaacctggt ggaattccac gagccccaat ataaagagat cgggctggcg
      361 ctggatcgcg cctttaagaa aaacgtgcaa aagcatgagc gcctggaaaa gcaaaacgcc
      421 gagcaaacgc tggaaaatgc cgttagagac ttaaatctgg ctgccgccaa agacctgata
      481 tggactccgg atcacggatt tatcgaaagc ggcgatcgca atttcgggct gcacagagac
      541 caactgaggc tatttaccag cagtgccgat ggtagacata tagatgaggg cttcaacgaa
      601 tatctcgcac tcaaaaatga agatgatgac gagttagaac caaatctcat tctcgatccc
      661 catgaaagag ccctggccga atgggaaaac ttcatgaaaa aggatccgca ggacgaccag
      721 gacagttatg aaatagggtc tgagagcggt cagggcggct gcttcgaaaa ctccgacgac
      781 tattttgacg atgattatct ttacggcacc atgagtggtg gggattaaca gtccgagaca
      841 ggcatctccg tgcgaggccg gctgcaaaac cggcatttaa cgcccatcga cgttttcaag
      901 cggcatcaaa ttaatatatt ttccttctga tttgatggat ctaaaatatt tagcgacact
      961 caagctctag aatcaatgca attagggcaa atcgcataat attaagcggt atgggcccgg
     1021 ctggagggat tgcctgcaat cttcgccggt tcatagagtc aattaatgga tgcagtgaga
     1081 agtcgatatg aacgccatcg ataagaaaga acgtagagta gttgttaccg gtataggcat
     1141 gatctcgccg gttggtcaaa gcgcaaaaga atgctgggaa gctctcttat cgggcacttc
     1201 cggcgtaggc tctctgacgt tgtttgatcc ggcagcacac ggcattgaaa caagaatcgc
     1261 ggctgaagtg aagaacttca acattcaaga tttctacccg gacaagcgta aagctggtaa
     1321 ctttctcaag gaaatggaca gggtaacctt gtttgccatg gccgcagcca aactggcagt
     1381 cgacgacgcc gcactcaaaa tcgatgaggg caacgccgag cgggtagcta ccttcatcgg
     1441 taccggggtc ggggggctca ccacaacgct tcaagactac cagaggctct tggagggagg
     1501 tcctcgcaag gtaggcgtgc gctcagtgat caagttaatg ccgaatgcac catccggcca
     1561 ggttgccatc gaatttggtg ctaaaggacg agccaaaggc gatgccacgg cctgcgcatc
     1621 cgggcttgac tcgctactcg atgcctttat gtatatccag gacaatcgcg ccgacgttgt
     1681 catcgccggt ggtgcagaag cggccatcaa tgctatcggt gtcacctcct tcggcaatat
     1741 gatggccctt tccaagcgca atgacgagcc tacgaaggca agccgcccct tcagcgccga
     1801 ccgagacggt ttcgttatcg gcgaaggagc agcggttctc attcttgaat ccctggagca
     1861 tgccagagct cgcaacgcaa agatctacgc cgagataaaa ggcggtgccg ccagttgtga
     1921 tgcccaccat atagtggctc ccaatcaatc aggcgaagga gccgccagag ccatagcgga
     1981 agctctgaaa gatgccggtc tcgaacccac cgaaatagac tacatcaatg cccacggcac
     2041 ctccactccc ctcaatgacg cttgcgaaac caaagccata aagacggttt tcggtgacta
     2101 cgcgcacaaa ctggccatct catccacaaa gtccatgatc ggtcaccttc tgggcggagc
     2161 cggagccatg ggagcggcag taacagcact ctccataaaa gattccaaag tgcatccgac
     2221 catcaactac gacaatccag atccggaatg cgatctagat tatgtgccca atgtcagtcg
     2281 ccagttgaat gtcagaaacg ccctggtgga agccctgggc ttcggcggac acaataccgt
     2341 actggtcatg ggtcaactga aagactgaac gctaagctag gcagtaatag tcttcttgcg
     2401 gggcggtccg ggaatctttt ccttctcggc ttccttagcc ttgccggcgt cagctactac
     2461 cgcatttccc gtcaagacgg aagcgggaag cggaggcaca ttgctgccgg aagacacttg
     2521 cacaggtgca gcgctctgtt gtccgtcttc atcgtcatca tcctggttgc cgttattggc
     2581 tgaagccaca acgccgcttc cggccttatc cacatcatcc tcagcctctt ccataccggc
     2641 cagttttctt gcatcatgac cattgccgcc ctctgcttcc gaggctatga gcgaattgta
     2701 gaggtgacca tcattgacaa gcttagaagt accggaggca ctgacgatgc cctgaccgga
     2761 gccaagtgct gtgctgttgc tggaataagg atctcttctg ccgctgaagc tcaagttgag
     2821 gagccgggca tcggtagcat tcaaaatcga gggtatgacg aattgactgc tggcaggttt
     2881 atccagccgc gaatccaaac gaccgtcacc attgtatgtg aatgtgacgg gaaaactcac
     2941 ggcacgagca attgggacgg catcaacggc actgggtaga gcactctgt
//
LOCUS       DAOQNZ010000370         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17489_length_2980_cov_4.019145, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000370 DAOQNZ010000000
VERSION     DAOQNZ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17489_length_2980_cov_4.019145"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKW73_07715"
     CDS             complement(<1..336)
                     /locus_tag="PKW73_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA73209.1"
                     /translation="MQYRKVGKWGVKVSEVALGSWLTYGGTVEEKNAVKQIHKAFELG
                     INFFDTANVYAQGQSEVVVGKALKTMKREACFVATKVFFPMGEGPNDKGLSRKHVFEQ
                     CNASLKRLGQ"
     gene            complement(405..1670)
                     /locus_tag="PKW73_07720"
     CDS             complement(405..1670)
                     /locus_tag="PKW73_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA73210.1"
                     /translation="MTNKPSLYIPTLYFIEGLPYAIVCSVSIIVFQNLDYSKDFMAQV
                     TSLLALPWTLKFAWAPLVDMLGLKRQWILVSHVVLAVCAVLLALSLQLEGGINFIILA
                     FALMAFTSATQDVAMDGYYLEVLDKEQQSVFVGVRNAAYRMAMLFGQGFLVYLAGTLS
                     HQYGIRAGWGAAFALSAVIFALAFALHAWLLPKPTGAASTASSKVDGGEFLTVFGSFF
                     RRPGIFVIVFYMMFFRLGDALMLKMAQPFLLDKAGAGGLQIATETVGLIYGGVGVGFL
                     LLGGIVGGYVVSKLGLKRTLMPTALIQNSAILLYYILSILKPGVVLVAVFNAFEQFAY
                     GLGLTAYTVFLLSLASPRYRSGHYAIATAFMALGLLLPGYVSGKLCTMLGYQNFFLLS
                     FLLSIPGMIAILFLPLGSLSQEAAKDKQD"
     gene            1735..>2980
                     /locus_tag="PKW73_07725"
     CDS             1735..>2980
                     /locus_tag="PKW73_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA73211.1"
                     /translation="MHSKNVAVLLTLSSFLLAAPALAEKRKPVLNSTLLEKRDFREPE
                     ITPESKQLNRAKEVDRQVRKLFFDQDKLEKIWLQDRSSLFKAASLVRSTRAIKPIINF
                     YLSKLGTSHCLFTTDEDESFYYLESLFGALQKKGNTKEYGVPGFVTGGAGFPEKTVRY
                     VLAGSAADKAGIEVGDEITSVNGKTEYTYKDCLAAAGTELTISYKRQGRDKTARLKPK
                     QMDHFKAYLQAMEDSLKTIIREVEIGGKKREVTLTYIHMYSGGPQVRETLESLLNDKS
                     TSDALILDLRDGYGGASLTDIDCLFRNPANYPVFEQRAAGDAKYVQNLCFTRPVVALI
                     NSGSRSGKELLAYTLKKTGRATLVGECTAGYVVAGCFQNIDEHCALYIACADCTIDGK
                     RLEGVGVEPDIKILNDSRDAKGY"
BASE COUNT          870 a          765 c          729 g          616 t
ORIGIN      
        1 ctgccccagc cttttcaaac tggcattgca ctgctcaaaa acatgcttgc gagaaagccc
       61 cttgtcgttc ggtccttcgc ccatggggaa aaagactttg gtggccacga aacaggcttc
      121 tcttttcatg gtcttgaggg ccttgcccac taccacttcc gactgaccct gcgcataaac
      181 gttggccgta tcaaagaaat tgatgcccag ttcaaatgct ttgtgaattt gcttgacggc
      241 attcttctct tcaaccgttc caccataggt gagccaacta ccaagggcca cttccgaaac
      301 tttcacgccc cacttgccga cttttcggta ctgcactgaa gaatcctctg aactttagag
      361 atggtctttt actaaatcaa agcaacaaac taaaagacct tatctcagtc ttgtttgtcc
      421 ttagccgcct cctgggataa cgagcccaga ggcagaaaca atatagcgat cataccaggg
      481 atggatagaa gaaagcttag gagaaagaaa ttctgatatc caagcatggt acacagcttg
      541 ccgcttacat agccgggcaa caaaagcccc agggccataa atgccgtagc gatggcgtaa
      601 tgcccgctgc gataacgggg tgatgccaag cttaaaagaa agacagtgta tgcggtcaaa
      661 ccaagtccgt aggcaaactg ttcaaaagcg ttgaaaacag ccacaagtac tacgcccggt
      721 ttaagaattg aaagaatgta ataaagcaaa atggcgctgt tttgaatcag ggccgtcggc
      781 attaaagtgc gcttaagtcc cagtttgctg acaacataac cacctacgat accacccaat
      841 aaaaggaagc ccactccgac gccgccatag ataagcccca cggtctcagt agctatttgc
      901 aaaccgcctg cccctgcctt atcaagcaaa aacggctggg ccatcttgag catgagggca
      961 tcgcccaggc gaaaaaacat catgtaaaag acaattacaa aaatgccggg tcgccgaaaa
     1021 aagctgccga aaactgttaa gaactcacca ccatcgacct tgctggaagc cgtactggct
     1081 gctccggtgg gcttgggcag aagccaggca tgcagtgcga aggcaagcgc gaagatgacg
     1141 gctgatagag cgaaggccgc cccccagccg gctcttatcc catactgatg agaaagggta
     1201 ccggctagat agacgagaaa gccctggcca aagagcatgg ccattcgata agcagcattg
     1261 cgcacaccca caaagacaga ctgctgctct ttatcgagta cttccagata gtagccgtcc
     1321 atggccacgt cctgtgtagc ggaagtaaaa gccatgaggg caaaggccag aatgatgaaa
     1381 ttaatgccgc cttcaagctg aagactcaaa gccaaaagca cggcacaaac agccagcacc
     1441 acatgcgaca ccagaatcca ttgtcgcttg agaccgagca tatccaccag aggcgcccag
     1501 gcgaatttta aagtccatgg caaggccagg agacttgtga cctgagccat gaagtctttg
     1561 gaataatcaa ggttttggaa gacgatgatg gagaccgaac aaactatggc gtagggtagg
     1621 ccctcgataa aatacagagt cggtatatat aaggacggct tgttggtcat ttatcttctt
     1681 gggtagagta aaggtttatt ctactgataa ttccgcttcc ctggatatcc tcatatgcat
     1741 tccaaaaacg tagctgtttt acttaccctc tcaagttttc ttttggctgc tccggcactg
     1801 gctgagaaac gaaaaccggt ccttaactcg actctattgg aaaagcgaga cttccgtgag
     1861 cctgagataa cacctgagag caagcaactt aacagggcta aagaggtaga tcgccaagta
     1921 agaaagctct tctttgacca agacaagtta gagaaaatct ggttgcaaga cagaagcagt
     1981 ctgttcaaag ctgcgagtct agtcagaagc actagagcaa tcaaacccat catcaatttc
     2041 tacctttcaa agctgggcac aagccattgc cttttcacca ccgatgaaga cgaaagcttc
     2101 tattacctag agtccctctt cggagcgctg cagaagaaag gaaatacaaa agaatacgga
     2161 gtacctggtt ttgtcaccgg cggggctggc tttccggaaa aaaccgtacg ctacgtgctg
     2221 gccggcagcg ccgccgacaa agccggaatt gaggtaggtg acgaaatcac ctcagttaac
     2281 ggtaaaactg aatatacata taaagattgc ctggcggctg ccggtacgga actgaccatc
     2341 tcttacaaga ggcagggtcg agataaaacg gccaggctca aacccaaaca aatggatcat
     2401 ttcaaggcct acctgcaagc catggaagac agcctcaaga caatcatcag agaagtagag
     2461 ataggcggca agaaaagaga ggtcaccctc acttatatcc atatgtacag cggtggtccc
     2521 caggtgaggg aaaccctgga gtctttgctt aatgacaaaa gcacaagcga tgccctcata
     2581 ctggacctgc gagacggcta tggtggtgcc agcctcactg atatcgactg cctcttccgc
     2641 aacccagcca actacccggt ttttgaacag cgggccgcag gcgatgcaaa gtatgtgcaa
     2701 aacctctgct tcaccagacc ggtggtggca ctgatcaatt caggcagcag gagcggcaaa
     2761 gagcttctgg cttacacact gaagaaaacc gggcgggcca ccctggtggg agagtgcaca
     2821 gccggttatg tggtagcggg ctgtttccag aatatcgacg agcactgtgc cctttatatc
     2881 gcctgcgccg actgcaccat cgacggcaag cgcctggagg gcgtaggagt agaacccgac
     2941 atcaagatcc tgaacgatag ccgggatgca aagggctatc
//
LOCUS       DAOQNZ010000371         2972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17590_length_2972_cov_2.882071, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000371 DAOQNZ010000000
VERSION     DAOQNZ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17590_length_2972_cov_2.882071"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..546
                     /locus_tag="PKW73_07730"
     CDS             214..546
                     /locus_tag="PKW73_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNA73212.1"
                     /translation="MFKPSERQGKKGITLDLKVSPGAKTDEFSGVHGGRLKVKVAAKP
                     LEGAANEALLRFIATSFAVPLASVQLIAGKSARMKTVFVASTAGDAGGQEKDQKRFVH
                     IAEKLAGH"
     gene            complement(552..866)
                     /locus_tag="PKW73_07735"
     CDS             complement(552..866)
                     /locus_tag="PKW73_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73213.1"
                     /translation="MSEEENPNPPSEQAAAKAPRRVLAPKPKPVAGTEKKEDNKVVKL
                     LEKNPLGMTLPEMAGGTRQLKKIRTLRPQLAEALNPGLVMPISQRAGHTVYKLVKHMG
                     PR"
     gene            1228..2856
                     /locus_tag="PKW73_07740"
     CDS             1228..2856
                     /locus_tag="PKW73_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNA73214.1"
                     /translation="MLHSHLPYYRKAGMWPFGEESVYECIAESYIPLLNAIGDLADEG
                     IQAKLTIGITPVLAEQLSDEHFKAGFDKFIADKIEAAQKDEKLYSVRGENSNPNKLLL
                     ARFYVNLFQHIHRDWNERWNRDLIGAFKRFQDSGCIEVATSAATHCFSPLLETDSAIF
                     AQYKTGVETYKRHFGRDPKGFWLPECAYRPASGKRPPLEKWLHELGLQYFFTESFVIK
                     GGQSAEMRRVIGPYGSIEYIPAAPRPETGLDTFEAFWLKEYPVAVMGRHEEAGYLVWS
                     ADQGYPGDGNYREFHKKDDNSGLHYWKLTSKTTDLGAKQLYNPEAAMARLNENSDHYV
                     GFLQQCLTDHLKKTGEPGLVMASFDTELFGHWWFEGVTWIKEVVKKLRQYTAVTMRTA
                     SEYLETQAPTKAIELPESSWGSGGHWQVWLNNETEWMWPIISECEHTTETLCQMFENT
                     NNDLARRAIKQLCRENLLIQSSDWPFLVTTGQAKQYAVERFNGHYERFKELAKMLREN
                     NIDGGKLSEIESIDNLFPDVSPSWFLSQQVSTPA"
BASE COUNT          769 a          753 c          754 g          696 t
ORIGIN      
        1 cccatctcga aatcagcgat ttttatcgac acactaaatc ctccaaagct gggctcattc
       61 taccaagtgc aatcatcaag ccactgtatc tgagaggctt tgaatcggga gtttgattta
      121 agataggcat atttggtcct ccataatcgc ctctcgattc ttcaaataca ggccctggca
      181 agggtttcgc ccacaccttc aggaaaacga aatatgttca aaccatctga gcgccaaggt
      241 aagaagggta taacactgga cctcaaagtc tcgccgggcg ccaaaacaga cgaattttcc
      301 ggtgttcatg gcggcagact aaaagtgaaa gtggcggcta agccgctaga gggcgctgcc
      361 aatgaagctt tgcttcgttt catcgcgaca agcttcgcgg ttcctctcgc cagcgtccag
      421 ttgattgccg gcaaaagcgc gcggatgaag actgttttcg tcgccagcac ggcaggcgat
      481 gccggcgggc aagaaaagga tcagaaaagg ttcgttcaca tagctgaaaa acttgccggc
      541 cactgatggt tttaccgcgg ccccatgtgc ttgaccagct tgtaaaccgt gtgaccggca
      601 cgctggctga tcggcatcac caatccggga ttgagcgctt cggccaactg cggcctgagc
      661 gtacgaatct tcttgagttg ccgggttccc cctgccatct caggaagcgt catcccgagg
      721 gggtttttct cgagaagctt gacgacctta ttgtcttcct tcttctctgt tccggcgacc
      781 ggcttgggct tgggagccaa aactcgccga ggtgctttgg cagcagcctg ctcgctaggt
      841 ggattcggat tttcttcttc tgacatcaag gtatccctat tgtcttcggt cgtctttcgt
      901 tcaaggtcgc cctgccatgc tgaggactgg atctgagcca atcatcaaat agtgcgaaca
      961 tcggcacatc cgcttgagca ctcaaataca aggataccgc ttcttttcaa gaatagttac
     1021 ggattaccag ttttttgatg acacgcttac tctcttattg atatatttcg gcctgttcct
     1081 atattgcaac aaccagccta tccgtgtgtt gcaactcgtt tgctattgga tgtctgaggc
     1141 gggcttccgc catggcaaag tctccgaatt acaggactaa caggacctga gaggtatatt
     1201 tgagtgagtg tcggttcatt tgtcttcatg ctgcatagcc accttccgta ttacaggaaa
     1261 gcaggaatgt ggccttttgg tgaagaaagt gtctacgagt gcatcgcgga aagctacatc
     1321 ccgctgctca atgccatagg agacctggca gatgaaggca ttcaagcaaa actgacaatc
     1381 ggcatcactc cggttctggc agagcagctt tcggacgaac acttcaaggc cggtttcgac
     1441 aaatttattg cagacaaaat cgaagcagcc cagaaggacg aaaaacttta cagcgtacgc
     1501 ggcgagaatt cgaatccaaa caaactcctt ctggcccgct tttacgtcaa tcttttccag
     1561 catatccaca gggactggaa cgagcggtgg aacagagatc tgatcggagc tttcaagcgc
     1621 tttcaagatt ccggctgcat cgaggtggca acttccgcag ccacacactg cttttcacct
     1681 ctgctggaaa ccgattcggc aattttcgcc cagtacaaga cgggcgtaga aacctacaaa
     1741 cgccacttcg gccgcgaccc caaaggtttc tggttaccgg aatgcgctta cagaccagcc
     1801 tcaggcaaac gaccgccgct ggaaaagtgg ctgcatgagc tgggtctgca gtatttcttt
     1861 accgagtcct ttgttatcaa aggcgggcag agcgccgaga tgcgcagagt aatcggacct
     1921 tacggttcaa tcgaatatat tccggccgcg cccagaccag agactggact ggatactttc
     1981 gaagctttct ggctgaaaga atatccggtt gccgtcatgg gcaggcatga agaagcaggt
     2041 tatctggtct ggtcagcaga tcagggttat ccgggcgacg gcaattaccg agaatttcac
     2101 aagaaagatg acaattccgg tttgcattac tggaagctga cttccaaaac caccgatctg
     2161 ggagccaagc agctctacaa tccagaggcg gccatggccc gcttgaatga aaattcagat
     2221 cattatgtcg gcttcttgca gcaatgcctc accgaccact tgaagaagac tggcgagcca
     2281 gggctggtaa tggcaagttt cgacacagag ctattcggac actggtggtt cgaaggtgtg
     2341 acctggatca aagaagtagt caaaaagctt cgccagtaca cggcagtgac aatgcgcacg
     2401 gcatcggaat atctggaaac acaggcaccg acgaaagcta ttgaattacc cgaatcgtca
     2461 tggggttcag gaggtcactg gcaagtctgg ctgaacaatg aaaccgagtg gatgtggccg
     2521 attatttcag agtgcgagca caccacagaa acactctgcc agatgttcga aaacaccaac
     2581 aatgacctgg cgaggcgcgc tataaaacag ctttgccgcg agaatcttct gattcaatca
     2641 agcgattggc ccttccttgt cactaccggt caagcgaaac aatatgccgt cgagcgcttc
     2701 aacggacatt acgagcgctt caaagagctg gctaagatgc tcagagaaaa caatatagac
     2761 ggcggcaaac tgtctgaaat tgagtcaatc gataacctgt tccctgatgt ttctcccagc
     2821 tggttcctgt cgcagcaagt ctcgacaccg gcgtagcggc atagcaaata gatcaggaca
     2881 gttagaacga ttttctgttg caaaaaagag agaaaacaat tcgaagaaga tgatgatgat
     2941 tgccaggagg aggactctgt ttggtgatcc tt
//
LOCUS       DAOQNZ010000372         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17663_length_2965_cov_5.414433, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000372 DAOQNZ010000000
VERSION     DAOQNZ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17663_length_2965_cov_5.414433"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKW73_07745"
     CDS             <1..643
                     /locus_tag="PKW73_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73215.1"
                     /translation="GTPYIQCVSIEYCKPLPRGHYTAFSQKGKVFLHCSSRIVARIPR
                     EPGNASSHCLETPSGMEISVNNRFGTLEIFAAEFSSPLLELFETGWKLFPAGDPLPDI
                     HPGCPSNRLAGDGSSALYFFRSGGGSVSMPLENFPTPSRVGWKIAVGYTAASVLCPSG
                     VFGLVMLLGFFEPFLACFSWSGFGVSMVWKFVSIPAGKTSSRFRHLICYRGAK"
     gene            1011..2831
                     /locus_tag="PKW73_07750"
     CDS             1011..2831
                     /locus_tag="PKW73_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73216.1"
                     /translation="MPTINGLAQARLASATAPTRFDRLQYFPKGWETCVAQFLELFHR
                     FGYIYKPLSGGSWYSANENWKLPDSEILKAIACAHPKFYIGCRAAKTTRFAVLDIDQN
                     SRYHNKQSLEKLLQCLSQAGLSRSSLYRSSYSGGWHLYLFFEEPIASLELRRQLFKLL
                     TLNDFQVGKGQLEIFPHPGGEGSMGLGLRLPMQPGFAWLDKKTLEIEHERNELDATYA
                     LELFLDVLDGDANSFEDFRRLKAHVQELEKRKASASVHGRGERVNNVVPIRAGMKAVP
                     ESEFSDFVRGVFHRLPPGIIVENWYKGRLYHLNGLNGPSQRAEAIECVGHYFFYGDPS
                     RDLPALGYGYEQERQWAIDEFLHTRNNGQSEDINRGRADAYAQVERAASWRPPHKQST
                     EPVKYAPERPISWIRENANRKTDARKRISDALEGLKKMQRSFTTVELQKAAGCSRETL
                     YNHADIWRQDYEDLAEGFFAICTDEYNDVVGAGSTQTQPPTTLSNPDMPPGRLAARRI
                     IYEISMRSQREQRQAEKTAHASQKASESSWLAEVTSLTKNDPSTLSVEEIKVLLVILL
                     GYLALAPDYDSQNTLQIYISALKEQLSSVLNGPQLVVRPP"
BASE COUNT          675 a          752 c          757 g          781 t
ORIGIN      
        1 cggcactcct tatatccaat gcgtatctat tgaatattgc aaacccttac cccgtgggca
       61 ttatacggct ttttcacaga aaggaaaagt atttcttcat tgctcaagcc gcattgtggc
      121 tcggattccg agggagcctg gaaacgcttc tagccactgt ctcgaaactc cttccggtat
      181 ggaaatctca gtcaataacc gattcgggac tctcgaaatt tttgccgccg aattttccag
      241 cccacttctt gagcttttcg agaccggttg gaaattattt ccagccggag atcccctccc
      301 tgatattcat ccggggtgtc cttcgaatcg cttggcagga gatggaagta gcgctctcta
      361 tttctttcga tctgggggtg gaagcgtttc gatgcccctg gaaaatttcc cgactccttc
      421 tagggtgggt tggaaaattg ctgttggata tactgcggct tctgttttgt gcccgtccgg
      481 tgtgttcgga ttagtgatgc ttttggggtt tttcgagccg tttctagcct gcttttcttg
      541 gtctggtttt ggcgtttcta tggtctggaa gtttgtttcg ataccggctg gaaaaacttc
      601 tagccgattc cggcacctaa tttgctatcg tggtgccaaa taatcgcaag cggcaataaa
      661 tgccgtcaaa ccatttctag gcttaagtat cgaaattttc cagccttcgg aaagccgccg
      721 ccattcgatc tgttcgggta gtaaagagtt gatcggtttt aacgcaaata atatagtgtc
      781 atcggtcgat gccaccaaaa gtggtgcata tcgaacggat atatagaggc tctagcctcc
      841 tagaaaggac aaagttaccg ataaaaaaac atcgcttggg gcgatgtctt ttttgagacc
      901 ttttgcggat ctcgttattc agttgttgtc gcgtttttgg aagtttcgcg aattgacttc
      961 aggatagacg aacggtccgg ataggtcaac tcatccctaa ttaactttgg gtgccgacta
     1021 tcaacggact ggctcaagcg cgcttagcat cagctaccgc gccgactaga tttgatcgac
     1081 tgcagtactt cccgaaaggg tgggagactt gcgtcgccca gtttcttgag ctgttccacc
     1141 gctttggtta catctacaaa ccgctctccg gtggctcctg gtactcggct aacgagaact
     1201 ggaagttacc tgattcggag atactcaagg ccatcgcctg cgcccatccc aagttctaca
     1261 tcggctgtcg tgccgcaaaa acaacccggt ttgcagtcct agatattgac cagaacagtc
     1321 gctaccacaa caagcaatcc cttgagaagt tgctacagtg cttgtctcaa gctggtcttt
     1381 ctcgatcttc actttatcgc tcaagttaca gtggtggctg gcatttgtac cttttcttcg
     1441 aggagcccat tgccagtctc gaactccggc ggcagctatt caaactactt acattgaatg
     1501 acttccaggt cggaaaagga cagctcgaga ttttccctca tccgggtggc gaaggctcta
     1561 tgggcttggg gctgaggttg ccgatgcagc ctggttttgc ctggcttgat aaaaagactt
     1621 tggagataga gcatgagcga aatgagcttg atgctaccta tgcgctggaa ttatttctcg
     1681 acgtactaga cggcgacgct aattcctttg aggatttccg aaggctcaag gcgcacgtcc
     1741 aggagctgga gaaacgcaag gcttccgctt ccgtccacgg tcgcggtgag cgcgtcaaca
     1801 acgtggtgcc tatacgtgct ggcatgaagg ccgttccaga gtcggaattt tccgattttg
     1861 ttcgaggggt gttccaccga ctgccgccag gcatcatcgt agagaactgg tacaaaggac
     1921 ggctttatca tctcaacggc ttgaatggtc catcccagcg cgctgaagct attgaatgtg
     1981 tcgggcatta tttcttctac ggcgacccga gccgtgacct gccagctttg ggatatggat
     2041 acgaacaaga gcgccaatgg gctattgacg agttcttaca caccagaaac aacgggcaaa
     2101 gtgaagacat taaccgtggc cgggctgatg cttacgccca ggtagagcgg gctgctagtt
     2161 ggcgtccacc acacaagcag tctaccgaac ccgttaagta cgcccccgag cgccccatct
     2221 cctggataag ggagaacgcc aatcgcaaga ccgacgcccg caagcgcata tcagatgccc
     2281 tcgaggggct caagaaaatg cagcgctcct tcaccaccgt ggagttgcag aaggccgccg
     2341 ggtgctccag ggagacgctg tataaccacg ctgatatctg gcgccaggac tatgaggacc
     2401 ttgctgaagg gttctttgca atctgtacgg acgaatataa cgatgtggtg ggggcgggtt
     2461 ctactcaaac ccagccccct accactcttt ctaatccaga tatgccgccg ggtcgcttgg
     2521 cagctcgccg gattatctat gagatatcca tgcgatcgca aagagagcag cgccaggcag
     2581 agaaaaccgc tcatgcctcc cagaaggctt ctgaatcatc ttggttggca gaagtaacca
     2641 gtcttaccaa gaacgatcct tcgacccttt ccgttgagga aatcaaagta ctcctggtca
     2701 ttttgttggg gtatctagcc ctggctcctg actatgacag ccagaacaca ttgcagattt
     2761 atatatccgc cctgaaagaa cagttgtcct ctgttctaaa tggtccgcag ctggtggttc
     2821 gtccgcccta actggggttc tttttcgaat ttcgttctat ttgatctgtc gatctgttat
     2881 gatgatgcca ctggatgtgg tgctcgatga cagtatcgcc agggagttct ctgaagtcgc
     2941 aagaagcgct tagtgagccc gataa
//
LOCUS       DAOQNZ010000373         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17671_length_2964_cov_3.578893, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000373 DAOQNZ010000000
VERSION     DAOQNZ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17671_length_2964_cov_3.578893"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKW73_07755"
     CDS             complement(<1..1144)
                     /locus_tag="PKW73_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73217.1"
                     /translation="MADEKDEQDKENLNQGNRPDLFAGIPGYTPHGFGQPTPAASEGI
                     NPNVEPAAAGPAAPLQPAQPQSNGENNYPQLKLPQSPQGATPVFPQLQLPPAPNPPAE
                     PRRELKPAPYVRRSDSTGTNMQALSPEQLQNMQAAADAAARSAPPQNPGNRPDPRQNI
                     SRVPLRPDVVMGEQSEAIPPRGSLEELHMINQQFISNEYVPKTPPVIVPHQDPDKPWA
                     AGAEPQFEFFAEEYEEALRKATRAREKKPDKDAPPTKPSVWQETFANVIQSQTESLQA
                     PQPAAKTVEPAQQPIPQGLNGEKSALSETFTKAPPKEISRGAAALRAAGFYDRNSTNQ
                     DTNPQPDAGFRMNRQDLVGILAFNLLTAKNILQSPRVFFSKMPTDGT"
     gene            1787..2170
                     /locus_tag="PKW73_07760"
     CDS             1787..2170
                     /locus_tag="PKW73_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73218.1"
                     /translation="MSTKALMRATDPSIGTNIGKTAVIKVKFSGDERGPVDPPQKVDS
                     SISAGYRSLMRDDTLAGRQHSRNFRNDERSESNSRLRMVSNLRQKESWLIWREFVRDL
                     DRSFGTMLQRFSPTMWLKDGKASYR"
     gene            complement(2252..>2964)
                     /locus_tag="PKW73_07765"
     CDS             complement(2252..>2964)
                     /locus_tag="PKW73_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNA73219.1"
                     /translation="LERLKQCHEVGGYLLSVVGTMYAVLLGLVVVDAMTKFQTARNVV
                     ENEANSLADVFLLAESMPKAKATAVRQLCEKYVSEVVEKEWPDMAKGQINKTARRHAI
                     NLMREVNAFEPVTENQKAIYPLLVQESCQVWDNRRARTNLASFGVPAVEWFVLILGGV
                     VTVVFTYFFGVESLLAQVLMTSMVSLLISLNMYLVVLFGAPFSGDLQVSSDALMVDKM
                     IFENQLGLRQDPEFSPKL"
BASE COUNT          627 a          779 c          800 g          758 t
ORIGIN      
        1 aggtgccgtc ggtcggcatt ttcgagaaga aaacgcgagg cgattggaga atgttcttcg
       61 ccgtcagaag gttgaaggcc aatatcccca ccagatcctg gcggttcatg cgaaagccgg
      121 cgtccggctg tgggttggtg tcttggttcg tggagttgcg gtcgtaaaat ccggccgctc
      181 tcagtgccgc agccccgcgt gaaatctctt tgggtggagc cttggtgaat gtctcgctga
      241 gcgccgactt ctctccgttt agaccctggg ggataggctg ttgagccggc tcgacggttt
      301 tagcggccgg ctgcggtgcc tgcaaggatt ccgtttgact ctggataaca ttggcaaaag
      361 tctcttgcca gaccgagggc ttggtgggag gcgcgtcctt gtccggcttc ttttccctgg
      421 ctcttgtagc cttgcgcaga gcctcttcat attcttccgc gaagaattca aactgtggtt
      481 cggcaccggc cgcccagggc ttatccgggt cttgatgcgg gactattaca ggcggcgtct
      541 tcggcacgta ttcattggaa atgaactgct gattaatcat gtgcagttct tcgagactac
      601 cgcgcggcgg aatcgcctcg ctttgctcgc ccatgaccac gtctggtctg agcggcactc
      661 gcgaaatatt ctgcctgggg tctggacgat tgccagggtt ctgcggcggg gcagagcggg
      721 ctgccgcatc ggcagcggcc tgcatgtttt gcagctgttc ggggctgagc gcttgcatgt
      781 ttgtgccggt gctgtcgctg cgtctgacat agggtgcagg cttcagttcc cgcctcggtt
      841 ctgccggcgg attgggtgca ggaggcaact gtaactgagg aaagacgggt gtagctccct
      901 ggggactttg aggaagcttc aactgcggat aattgttttc accattgctt tgtggctgtg
      961 ccggttgcaa cggggcggcc ggtccagccg ctgccggctc gacgttagga tttatgcctt
     1021 ctgatgcagc cggcgtcggc tgcccgaaac cgtgcggggt gtagcccgga ataccagcaa
     1081 ataaatcagg gcggttgcct tgatttagat tctctttgtc ctgctcgtcc ttttcgtctg
     1141 ccactggttt ggctgtttcc cagataagag gttcccgttt ggcgcggtct gaacatcgtc
     1201 gtcgcatttt ggaagcacga tgacagagcc tgcaatcgcc gcttctatca agcctagatg
     1261 aaatcccctg gttattctag tgggttgtca atgatttcat gtttgccatg gaaagccttc
     1321 ctgcccagaa gcgcctgaaa gccgtattga accggtagta accgagtgaa gaaattctct
     1381 caagtaaggg gctctttgaa tttggtatcg cgggtgctac caggacgaat ttgtgcttat
     1441 gcaaactgat cttctacgcg ggggcaccac ttgtcagcct cggcttctga actacttgta
     1501 tatattaatt ctcgatgtgc ctgtagtagt ctttctgcgg ggcggaacct tttacctgag
     1561 atgaaagtct gtgcattctg tgagggatac atgcagtttt ctttgggctt tcaagaggtt
     1621 gttgtaagta atgaagcccg ctccataaat ggacatcatc cttttatggt attgctctaa
     1681 gaatacttgt catgcataat agcaacgtgc caaaaccggc tctgtaagtg cgaaacttcg
     1741 ctttatccct cgcatatata ccggcgccaa tcaagaggta gcaaagatgt caaccaaggc
     1801 ccttatgaga gccactgacc cttccatcgg tacaaatatc ggcaaaaccg cagtcatcaa
     1861 agtaaagttc tccggtgacg aacgtggtcc cgtcgatccg cctcaaaaag ttgattcctc
     1921 aatctccgcc ggttatcgct ctctcatgag agatgacacc ctggccggta ggcagcactc
     1981 ccgcaatttc cgcaacgacg aaagaagcga gtccaattcg cggctgcgca tggtttcgaa
     2041 cctccgccag aaagaatcct ggctcatctg gcgcgagttt gtgagagacc tcgataggtc
     2101 cttcggcaca atgctgcagc gcttttctcc caccatgtgg ttgaaagacg gtaaagctag
     2161 ttacagatag tccatacata agatttgaaa acgccctgct cacaagcggg gcgtttctct
     2221 ttgctattcg gcaaacgaag accggtaaaa gctacaactt ggggctaaat tcgggatcct
     2281 gcctcaagcc aagctggttc tcgaagatca ttttgtccac catgagcgcg tcgctactga
     2341 cctgtagatc tccagaaaat ggtgcgccga agagcacaac cagatacatg ttcagtgata
     2401 tcaagagaga taccatggaa gtcatcagta cttgtgccag gagactctct acaccgaaga
     2461 agtaggtgaa caccacagtc accactccgc ccagaatcaa gacaaaccat tccaccgccg
     2521 gtacgccgaa gctggctaga ttagtacggg cgcgacggtt gtcccagacc tggcaagact
     2581 cctgaaccag cagcggatag atggcctttt gattctccgt caccggttca aaggcgttca
     2641 cctcgcgcat gagatttata gcgtgccgac gggccgtctt gttaatttga cctttggcca
     2701 tgtccggcca ttccttttcc actacctcgg aaacgtactt ttcacatagt tgacggaccg
     2761 ccgttgcctt ggccttaggc atgctttcgg ctagaaggaa gacatcggcc agactgtttg
     2821 cttcgttttc tactacgttt ctggctgttt ggaacttggt catagcatct accaccacca
     2881 gtccaagcaa aaccgcgtac atggttccca ccactgagag gagataacct cccacttcat
     2941 ggcactgctt gagtctttcc aggt
//
LOCUS       DAOQNZ010000374         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17693_length_2962_cov_4.148607, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000374 DAOQNZ010000000
VERSION     DAOQNZ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17693_length_2962_cov_4.148607"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PKW73_07770"
     CDS             <1..459
                     /locus_tag="PKW73_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNA73220.1"
                     /translation="FTRLADKIKAMVRRNFGEPLVLAFDGANLTCVKYAGFLHRLDIF
                     GRLLLLDSTSEENKAYVEENMAKLKLQDQVYVQGQNVGEAPTWLSNFKAFRRAAICTP
                     LLFIVGVLAYIPVLEFLIQGIYMLFEKNASFWLGPSIKPAKPMVSEGAAS"
     gene            456..1199
                     /locus_tag="PKW73_07775"
     CDS             456..1199
                     /locus_tag="PKW73_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73221.1"
                     /translation="MRKIFIVKALLIVFVFAIVIGGTFPVRQAAVENEKLNHYDTQIE
                     QNLELARVRYNRLLANPGANPDDLLNARSELATALWAKQEFQEAVQLYTMQMAATWGL
                     KPNAYNEKWVNACRQLADIHRDANQMEAALICYKSVYDHDSKYLPADDLRVVRDLNNL
                     AMAYYMRGTGFEEKDKRHKDFAQAETYLKQVLTLLEKAGLGKSSRAATAYWNLYLVVR
                     DQDRALDAESYRKLAEGLDKSMNRLCREP"
     gene            1248..2381
                     /locus_tag="PKW73_07780"
     CDS             1248..2381
                     /locus_tag="PKW73_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73222.1"
                     /translation="MQDIASSSGNDTSQIIEGEVTEACLPGSSPWILNPLLDYLFVAG
                     GMLWILWGLSALGTATKNDPTSMVYGAILFCGSLLISDVHGPATLARVYTSATTPNKT
                     RRLVTLAAVIFIALGLPCLFNPGLAAIFTKITLLWGVQHYTAQTYGVVMIYSMKRKFN
                     LAPFERLVVQNLLRAQILFVWTRACTYKEYGSLNFMGLDIPFWGPLPIGFLIFSQFAL
                     VLMMIAFVLTFAYRFYSERRLLPLPAILSIVSVITLTFLARDNFNYLIGVIFYHASQY
                     LAITYSYYLRELAIKRAQANPSTAPPTLKEVFFSRPSLLYYASILLVGLFITFLLPKG
                     IAFFGISFTLALCVVYSVFNCHHYLADSFIWRIRDEKVRQLLV"
     gene            2596..>2962
                     /locus_tag="PKW73_07785"
     CDS             2596..>2962
                     /locus_tag="PKW73_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA73223.1"
                     /translation="MSSTDTISVLIAEDEAVTRLGLKFALNTFPDLRLVSECADGIAA
                     ILQALAKHPRVILMDLGLPKVDGITASRKIKEALPQTKIIALTCAEDEHIILNALNAG
                     VDGYCLKKISGERLHEVITA"
BASE COUNT          693 a          835 c          715 g          719 t
ORIGIN      
        1 ttcacccgcc tggcggataa aataaaagcc atggtacgcc gcaactttgg tgaacctcta
       61 gttttggctt tcgacggcgc caatttaacc tgcgtcaaat acgccggttt cctgcaccgc
      121 ctggacattt tcgggcgcct gctcttgctg gacagcacca gtgaagaaaa caaagcctac
      181 gtggaagaaa atatggcaaa actgaagctg caagaccagg tttacgtgca aggtcagaac
      241 gtcggcgagg ctcccacctg gctctccaat ttcaaggctt tccgcagagc agcaatctgc
      301 accccgctgt tatttatcgt cggtgtgctt gcctacattc cggtattgga atttctcatt
      361 cagggcatct acatgctctt cgagaaaaat gcgagcttct ggctgggtcc gagcattaaa
      421 ccggccaagc ctatggtcag tgaaggagca gcctcttgag aaagatcttt attgtcaaag
      481 ctttgctgat tgtctttgtc tttgccatcg tcattggtgg cacattcccg gtacgtcagg
      541 ccgccgtgga aaacgaaaag ctcaatcact acgacaccca gattgagcaa aatctcgaac
      601 tggcgcgggt gcgttataac agactgcttg ccaatcccgg tgccaaccca gatgatctct
      661 tgaacgccag aagcgagctg gccacggccc tctgggccaa gcaggagttt caggaagcag
      721 tgcaacttta taccatgcaa atggccgcca cctggggtct caagcccaat gcctacaatg
      781 aaaaatgggt caatgcctgc cgccaacttg ccgacataca ccgcgatgcc aaccagatgg
      841 aagcagctct catctgttac aaaagcgtat acgaccatga ctcgaaatat ctgccggccg
      901 atgatttgcg ggtggtgcga gacctgaaca acctggccat ggcttactac atgaggggca
      961 cgggctttga ggaaaaggac aagcgccata aggactttgc ccaggccgaa acctatttga
     1021 agcaagtgct aacccttttg gaaaaggccg gtctcggaaa gagcagccgc gccgccacag
     1081 cttactggaa cctttatctg gtggtaagag accaggatcg agctctggat gcggaatcct
     1141 atcgaaaact ggctgagggt ctcgacaaat ccatgaaccg gctctgccgc gaaccctgat
     1201 gctataatgc aagaggctca ggctgagaga ttgggagagt tcattccttg caggacatcg
     1261 cctcttcttc cggaaacgac acttcccaga tcatagaagg ggaagtgacc gaggcctgcc
     1321 ttccaggctc ttccccatgg atcttgaatc cgcttctgga ttatctcttt gtggccggag
     1381 gcatgctctg gattctctgg ggcttatcgg cccttggtac agcaaccaag aacgatccta
     1441 cctcaatggt ctacggcgcc attctcttct gcggcagcct gctcatctcc gatgtgcacg
     1501 gaccggctac cctggccagg gtttatacat ccgccaccac ccccaataag acgcgccggc
     1561 tggtaaccct ggctgcggtt atctttattg ccctgggtct gccctgcctg ttcaatccgg
     1621 gcttagcggc catattcacc aaaataaccc tgctctgggg cgtacagcac tacacggcac
     1681 agacttacgg agtggtgatg atctactcca tgaagcggaa gttcaatctt gccccttttg
     1741 aaaggctggt ggtgcaaaat cttctgcggg ctcagattct ctttgtctgg accagggcct
     1801 gcacctacaa agaatacggc tctctcaact tcatggggct cgatatacca ttctgggggc
     1861 ccttgcccat aggtttcctg attttcagcc agtttgctct ggtcttgatg atgatagctt
     1921 ttgtgcttac ctttgcttat cgattttaca gcgagcgccg gcttttgcca ctaccggcga
     1981 tactaagcat tgtcagtgtc attaccctca ccttcttagc cagagataat ttcaattacc
     2041 tgatcggcgt cattttctat catgccagcc aatatctggc aatcacctat tcttactatt
     2101 tgcgcgaact ggccatcaag cgggcccagg ccaatccctc aactgcacca cccactctca
     2161 aggaagtatt cttctcgcgc ccttccctcc tttactatgc ctccatcctc ctggtggggc
     2221 ttttcatcac cttccttctg cccaagggca tagccttctt tggcatctcc ttcaccctgg
     2281 ccctttgcgt ggtttattcg gtcttcaact gccatcatta cctggctgac tccttcatct
     2341 ggcgtatacg tgacgaaaaa gtcaggcaac ttttggtctg aaacagcctc gcagaccgtc
     2401 acggctatga aacctaagat taaaagagtg tgtaagccct aaaacacctg ttccacaagc
     2461 agtgtagctg tcagaatgaa atagtcggat cgatttagtc cctccttctg gaaattggga
     2521 cggcacggaa caggcacaga gcaattggca ggttaacggc tggtggatgc ccacaggccg
     2581 acccgggaag gtactatgtc ttcaacagac actatatccg tgctcattgc cgaagatgag
     2641 gctgtaaccc gtcttggtct gaagttcgcc cttaacacct tccccgatct gcgcctggta
     2701 tccgaatgcg ccgacggcat tgccgccatt ttgcaagctc tggccaagca tccccgggtc
     2761 attcttatgg acctgggact gcccaaggtg gacggcataa ccgcttcacg caaaatcaaa
     2821 gaagcgctgc cccagacaaa aattattgct cttacctgtg ccgaagatga acacatcatt
     2881 cttaatgccc tcaatgccgg agtagacggc tattgcctca agaaaatctc aggagagcgg
     2941 ctgcacgaag tgattacggc gg
//
LOCUS       DAOQNZ010000375         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17694_length_2962_cov_3.898177, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000375 DAOQNZ010000000
VERSION     DAOQNZ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17694_length_2962_cov_3.898177"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKW73_07790"
     CDS             complement(<1..370)
                     /locus_tag="PKW73_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73224.1"
                     /translation="MLSTNEVENQLVQWETDIRQAVANVTELENLSTYQIICQRPVPA
                     ENLTGVSRAKVEPLVADMKQMWPHLSLVISTVQEARKLKNSSRRLSDRDLNEIDRLLN
                     GQSIKLPDTIVPLQKRGLVSA"
     gene            complement(389..1087)
                     /locus_tag="PKW73_07795"
     CDS             complement(389..1087)
                     /locus_tag="PKW73_07795"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA73225.1"
                     /translation="MTDADVSKGVQIFTLPELLQYFQAQPKSRRLIVMAGPQGSGKSY
                     LAKRLKRLRSDDCGYRLFKRLSADKLKKRSPWLTDAEAEREYMTALKRSLENAHNIVD
                     DNLNASDWVRKKVIDLARSHGYEHISIVYIDLPLARCLSRNAARSRPTADWIVRKVWE
                     KFYRDGLPQACEAEVIRIKPLDDRVSYELSVTEYKPTSTVAPSVKSPKVGLSVAIFQR
                     VLRFWRALTKGSNR"
     gene            complement(1113..2315)
                     /locus_tag="PKW73_07800"
     CDS             complement(1113..2315)
                     /locus_tag="PKW73_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNA73226.1"
                     /translation="MTNDTRDSNPRKGRSFLLLTGQMVLVIFLAIFVLAAAMIYGPSL
                     LLSFERLPLVMQIGVLCIGLAIAFDFVNGFHDTANAVATVIYSGALPAWLAILMSAVL
                     NYAGAMTVGTSVAEFIASTIPRDVVSGPMILATLLAGLTWNLLTWWKGLPVSSTHCLL
                     GSLAGAGFAVAGLNGFSHPAVLKALAALIVSPIIGFVLAFIFAKCMDFLVRHLSGKTT
                     SNGVEIARQSKVWRLIFKGLQIATSASVSFSHGANDGQKTMGLITLILGTIFVSYGYS
                     VGIVPDWVKISAALAIGLGTAIGGGRVIRTVGEKLSARAIDPVQGCAAEFTTAMTVLY
                     ASKLGVPASTTHVLTSSVVGSSISLHGKQFANWSTLKKVGLAWLVTLPFTALLAGGFY
                     FLLSSVLA"
     gene            complement(2404..>2962)
                     /locus_tag="PKW73_07805"
     CDS             complement(2404..>2962)
                     /locus_tag="PKW73_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73227.1"
                     /translation="RGAGLFLTVGEDCIMNTSSVLSVELSVVAFVVVALCLFGQRMKS
                     VHIWKAEHLWELAGKVGVSGFLGLVLSYLFSFNGADAGYGLASSALFMVWLLPFVLHD
                     FVAEWPSDKLYWQEKATKASALRSLLVLAFSLVLLSGGVYFGALNSSWSSSAGSITLV
                     MLAVVAAAVIWLSAHFEKVSEELWG"
BASE COUNT          763 a          834 c          765 g          600 t
ORIGIN      
        1 cagccgagac aagccccctc ttctggagcg gcacaatggt gtcgggcaac ttgatggact
       61 gcccgttcaa cagccggtct atttcattca aatccctgtc actgaggcgc cggctgctgt
      121 tcttcagctt gcgtgcttct tgcacggtgc tgatcaccag gctcaagtgc ggccacatct
      181 gtttcatgtc ggcaacaagg ggttctacct tagcccggct cacaccagtc aaattttcag
      241 ctggcaccgg gcgctggcaa ataatctggt aggtagacag attttccaac tccgtgacat
      301 ttgccacggc ctgcctgata tccgtctccc actgtacaag ttggttttca acttcgtttg
      361 tagataacat aatctgctct tctaagaatt agcggttcga gccttttgtt aaggcgcgcc
      421 agaaccgtaa tacacgttga aagatggcaa cggaaagacc gaccttcggc gacttaacgg
      481 aaggtgcaac cgttgacgtt ggtttatact ccgtgacgga aagctcgtag ctaacccggt
      541 catccagagg cttgatgcga attacctcag cctcacaagc ttgcggcaat ccatcccggt
      601 aaaacttttc ccacaccttc ctgactatcc agtcggcggt cggtctactc cgtgccgcat
      661 ttctagacag gcagcgagcc aggggaagat cgatgtagac gatggaaata tgctcatagc
      721 catgactgcg ggccaggtca atgaccttct tgcgcaccca gtcactggca tttagattgt
      781 cgtccacaat attgtgagcg ttctccagac tacgtttgag cgcagtcatg tactcacgct
      841 ccgcctcagc atcggtgagc cagggcgaac gtttcttgag cttatcggcg ctcaagcgtt
      901 tgaagagacg atagccgcaa tcatcactgc gcaaccgctt caagcgcttg gccagatagc
      961 tcttgccgga accctggggt ccggccatta caatcaagcg gcggctcttg ggttgcgcct
     1021 gaaaatactg caaaagttcc ggcaaagtga agatttgcac tcctttgctc acatctgcgt
     1081 cagtcatacg aaatcctcct caagagagcg gctcaggcga gcacgctgga aaggagaaaa
     1141 taaaatccac cagccagtaa agccgtgaag ggtaaagtga caagccaggc cagaccgact
     1201 ttcttcaggg tgctccaatt ggcgaattgc ttgccgtgca ggctgatgct gctgcccacc
     1261 acactggagg tcagcacatg ggtagtactg gcgggcacac ccagtttgga ggcatagagc
     1321 acggtcattg ccgtagtgaa ctcggcggca caaccctgaa caggatcaat ggctcgagcc
     1381 gagagtttct cacccacggt gcgaatcacc cgaccaccac caatggcagt gccaagtccg
     1441 atggccagtg ctgccgatat ctttacccaa tcaggcacaa tccctaccga atagccgtag
     1501 gacacgaaaa tggtgccgag aatgagggta atgagcccca tcgttttctg cccgtcattg
     1561 gcgccgtggg aaaagctgac gctggcggaa gtagcaatct gcaagccctt gaagatcagg
     1621 cgccagactt tcgattgccg cgcgatctcc acaccgttac tggttgtctt gccggacaga
     1681 tgcctgacca ggaagtccat gcatttggca aaaatgaaag ccaacacaaa gccgatgata
     1741 ggcgaaacaa tcaaggctgc aagcgctttc aagaccgccg gatgactgaa accattcagc
     1801 cctgcaacgg cgaaaccagc tccagccaag cttcctaaga ggcagtgggt gctggagacc
     1861 ggcaagcctt tccaccaggt gagtaaattc caggtgagac cggctagtag cgttgccaga
     1921 atcatcggcc cactgaccac atctctggga atggtggagg cgatgaactc ggccacggaa
     1981 gtgccgacag tcatggcacc ggcataattg agcacggcac tcatcaggat agccagccag
     2041 gccggtagcg cccccgaata gattactgtg gctacggcat tcgccgtgtc gtgaaatccg
     2101 ttcacgaaat cgaaggcgat ggccaatcca atgcaaagca ccccgatctg catcaccagc
     2161 ggtaagcgct cgaagctgag caggagggac ggtccgtaga tcattgccgc cgccagaaca
     2221 aatatggcca gaaaaataac cagcaccatc tgtccagtca gaagaagaaa ggagcgtccc
     2281 tttcgggggt ttgaatctct tgtgtcattt gtcattgctt attttctctt gcttggttaa
     2341 aagaagacgg ctgcctcaaa agtgcagccg tcagtcaggt taaaattcag agcgaatggg
     2401 tctttagccc cacagttctt cgctcacctt ctcgaaatga gcggacagcc agatcacggc
     2461 agctgccaca acagcgagca taaccagggt aatggagccg gcagaggatg accaggatga
     2521 gttgagggca ccgaagtaca caccgccgct cagcaaaacc aggctgaaag cgagaaccaa
     2581 aagggaccgc aaggcagaag ccttagtcgc tttttcttgc cagtagagct tgtcgcttgg
     2641 ccactccgct acgaagtcat gcaacacaaa gggcaacaac cagaccatga acaaggcgct
     2701 actagccaga ccatagcccg catcagcccc gttgaagctg aagagatagc tgagcaccag
     2761 acccagaaag cccgagacgc ctaccttgcc ggccaattcc cagaggtgct ccgctttcca
     2821 gatgtgcacc gacttcatgc gttgcccaaa caagcacaga gcgactacga caaaggcgac
     2881 aaccgacagt tcgactgaca atactgaaga agtgttcata atacaatcct caccgacagt
     2941 tagaaaaaga ccagccccac gg
//
LOCUS       DAOQNZ010000376         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17695_length_2962_cov_3.765050, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000376 DAOQNZ010000000
VERSION     DAOQNZ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17695_length_2962_cov_3.765050"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..893)
                     /locus_tag="PKW73_07810"
     CDS             complement(45..893)
                     /locus_tag="PKW73_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA73228.1"
                     /translation="MNGPSYLLTIDGSAESRSAAYFAWELARQTEARVVAQHVVDTRA
                     IWQFLSYDLPGFIGSGVYLDAKERITEVMKSVAESLMLAYTSQVSELSDNHETYIDEG
                     SPAEEIARRAREHDLVIVGYHGRGPSGRRRRFFETLAAKCPAPILVVRKVSKPWTKMQ
                     ILVSNKMSPDKDLADIYKMGARLGLPIEVIIEEGCGKAEEERFQIGGWVNSYGIIDIR
                     QKNLQEAITKAPADAVLVVDAEALTETDELFAARVRSYLEDSPHRALMIWRDHQAEIR
                     SEPLAS"
     gene            1258..2058
                     /locus_tag="PKW73_07815"
     CDS             1258..2058
                     /locus_tag="PKW73_07815"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNA73229.1"
                     /translation="MTTFFIAFALFYVYHAQGITLGYHRLLSHKSLQVPKWLEYLVVS
                     GGYLTLEGSPIFWVTTHRLHHRYSDHAGDPHAPKDGLWHAFVSWMWKPKVLISAEESQ
                     KIAPDLYRDPVYKFLHCNHTHWDGYLCLTFAVLFRLALYFAFGPVVLAANLLATAMSF
                     LGPLLVNSVGHLRQFGYETYPCGDDSRNVWFVAMLSMGEGWHNNHHAFPQSARHGLTW
                     KEPDPTWMTISVLKLLGIAKEIRLPKGQSHYAVAPKKEPVLAADAPAK"
     gene            complement(2200..>2962)
                     /locus_tag="PKW73_07820"
     CDS             complement(2200..>2962)
                     /locus_tag="PKW73_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA73230.1"
                     /translation="PTENAPRNRFTVVIGLSNFRDEKIPRLRYSGKDAGDVANFLKAE
                     AGFAESNIKLLLDDAATRRTILDTISDWLPARAGAGDIVFIFISSHGTPTYGEIGALN
                     SVVTYDTNLGELELFSTSLPMQKLLREVRAKLKKQRTFIVLDTCYAGGLGAPDGATSA
                     NIDPDLIVNSNLQLLLSSSETNERSWESKRYPNSVFTRQLIEALRGNLRYQDFHDIFA
                     SVRKNVQDEVASDRNSKQTPAMAGKWTGQGLARDK"
BASE COUNT          591 a          764 c          805 g          802 t
ORIGIN      
        1 ggttatagcc aggctgagat ggactgcccg gctcgccggg aagcctaaga tgccagaggt
       61 tcgctgcgaa tttcggcttg gtggtcgcgc cagatcatga gggcacggtg cggcgagtct
      121 tccaggtagg aacgcactcg tgccgcaaag agttcgtcgg tttcggtgag cgcttcggca
      181 tccacaacca gtaccgcatc tgctggtgct ttcgtgattg cttcttgcag gtttttctgg
      241 cgaatgtcga tgatgccgta ggaatttacc cagccaccta tttggaagcg ttcttcttcc
      301 gctttgccgc agccctcttc gatgattact tctatgggca agcccagtct ggctcccatt
      361 ttatagatat cagccaggtc tttgtccggg ctcattttat tgctcacgag tatctgcatt
      421 tttgtccatg gttttgatac ttttcgaacc acgagaatcg gtgccgggca ttttgctgcc
      481 agggtttcga agaagcggcg gcgtctgccg gatggacccc ggccgtggta gcctacaatg
      541 accaggtcgt gttccctggc ccgtctggcg atttcttcgg cgggactgcc ttcgtctatg
      601 tatgtttcat ggttgtcgga aagctcgctt acctgacttg tgtaagcgag catgagggat
      661 tcggctaccg acttcattac ttcagtgatg cgctctttgg catctaaata gacgccgctg
      721 ccgatgaagc cgggcagatc gtaggagaga aattgccata ttgccctggt gtccaccaca
      781 tgctgggcaa ctactctggc ttcggtttgt ctggctagtt cccaggcaaa ataggcggcg
      841 gagcgcgact cggcggagcc gtcaatggtc aataaatagg atgggccgtt catggtaaaa
      901 acctcgcttt tatatagtcg attgagccaa tggttaccta gcccccgacg ccgcatcagt
      961 gtcagtgatt gtcgtccgtc aaacaagaaa gcagcgcaaa atacacggag cgcacaagtc
     1021 gctccggtgc ctgtcttctt ttcgcatggt aaggccgggg gttgatttgt aaatccatcc
     1081 aagtagcgat tcttgacggt aaccggttac aaaaaggtgg ccgctgtaaa gtgcggcggc
     1141 ggcaagctaa gctaggtctt caatctggag tttgcatggc gcagggctga tggccggtaa
     1201 cctccggagg ttcgaaccga tcgatgaaca cttagatgaa tcagcatgga tgaaattatg
     1261 accacgttct ttatagcctt tgcccttttc tatgtttacc atgctcaggg tattaccctt
     1321 ggctatcacc gtttgctttc tcataagtct ttgcaggtgc ctaaatggct ggagtacctg
     1381 gtagtctccg gaggctattt gaccctggaa ggatctccca ttttctgggt gacaacccat
     1441 cgcttgcatc accgctactc tgatcatgcg ggtgaccccc atgcccctaa agatggcttg
     1501 tggcatgcct ttgtctcctg gatgtggaag ccgaaggtgc tcataagcgc tgaggaaagc
     1561 cagaaaattg ctcctgatct atatcgcgat ccggtctaca agtttttgca ttgcaatcac
     1621 actcactggg acggttatct atgtttgacc tttgccgtcc tctttcgttt ggcgctctat
     1681 tttgcctttg gtcccgttgt tttagctgcc aatctcctgg ccaccgccat gtctttcctc
     1741 ggtccgttac tggtaaattc tgtagggcat ttgcgtcagt tcggctatga gacctaccct
     1801 tgcggagatg acagccgtaa tgtctggttt gtagccatgc tctctatggg cgaaggttgg
     1861 cataataacc atcatgcctt tccgcagtcg gccaggcacg gtctcacctg gaaggagccg
     1921 gaccctacct ggatgactat ttccgtactc aaactgcttg gaattgccaa agagattcgc
     1981 ctgcccaagg gacagtcgca ctatgcagtg gcacccaaga aagagccggt gttggctgcc
     2041 gatgctcctg cgaaataaag ttttccaccc gtaacaaatc agggcaaggg cggtaccgaa
     2101 tgcggtatcg ccttttcctt tctccggacc agcttgaaag agcctgggtc tgagtctggg
     2161 tctgggtttg gcttgtccac tctctttccc tatttatccc tatttatccc tggccagtcc
     2221 ctgaccggtc catttgccgg ccatggccgg tgtctgtttg ctgttccggt cggaggccac
     2281 ttcatcctgc acgttttttc gcaccgaggc aaagatgtcg tgaaaatcct ggtagcgcag
     2341 gttgcccctc agggcttcga taagctgtct ggtgaagacg ctgttcgggt agcgctttga
     2401 ttcccaggac ctttcgttgg tttcactgga actcaaaagc agttgcaaat tggaattgac
     2461 gatcaggtcc ggatcgatat tggcgctggt ggcaccatcg ggggcgccca gtccacctgc
     2521 gtagcaggtg tcgagaacta tgaaggtacg ctgtttcttg agcttagccc ttacttcgcg
     2581 caggagcttc tgcatgggca ggctggtgga aaagagttcc agctcaccca ggttggtatc
     2641 gtaggttacc actgagttga gggcgccaat ttctccatag gtaggcgtgc catgggagga
     2701 aatgaatatg aatacgatgt cgcctgcccc ggccctggcc ggcagccagt cgcttatggt
     2761 atcgagaatg gtgcggcgtg tggcggcatc atccagcaag agtttgatat tgctctctgc
     2821 aaaaccggct tctgctttca aaaagttggc tacatcaccg gcatctttgc ctgaatatct
     2881 aagcctgggt attttctcgt ccctgaagtt cgaaagaccg atcactacgg taaatctatt
     2941 ccggggggca ttctctgtag gc
//
LOCUS       DAOQNZ010000377         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17713_length_2961_cov_3.156917, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000377 DAOQNZ010000000
VERSION     DAOQNZ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17713_length_2961_cov_3.156917"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="PKW73_07825"
     CDS             complement(<1..1741)
                     /locus_tag="PKW73_07825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA73231.1"
                     /translation="MSSDKVIKDGALIFLLALALRLTYLFAIEHLVVQEGDAFYFLSG
                     GSELLNFAHSILSGKLNSLEAALAALGQVGATDAQVMSSAKLADRFMNDGPVITTYMA
                     VVNLLAGVPPGSKDFNAHIWNLGTIISIVSSLSCVCTYLLARRFFGRLTAILAALFLA
                     FYPSACLNVQSCYSEIFCSALLPLWLLFVDRLKERRSRAPLDGPVVFLGFLTAALMLS
                     KPLFYLLPPAVALAYLLTGRNGLRSGLKTMSLKALPFCLGLALVFAPWLFITRAIAGK
                     PLLTVTRSPAFNLFLGSDLERDGWRAYPFQRDIPTVPADAINRLFLSFKQEPLAVASM
                     EVRKVPRLLAGSWNEFRYKVFGLDSAQQDFIHRLLLLLGLSGLMLTLSARDKNQCRFA
                     LLSLLVLAAHLSYCAFEPISRYNYTAIPILAILAAHCLVRLGPTTALPALLSLSSLLV
                     LILSSYRTLAVLMASPSVLGNGELELITASICALSVLAFAGFLCRTSAGTARPFKATL
                     AALTIFGSAAVCTFFCVLNDRELYTVSHSLLRPVQTNLALSDLPAGDKVDLLLDLESP
                     VSPLSLLFAVNGRV"
     gene            complement(1748..>2961)
                     /locus_tag="PKW73_07830"
     CDS             complement(1748..>2961)
                     /locus_tag="PKW73_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNA73232.1"
                     /translation="KHMTNKSITWRKEGFSRSYFDGAGLREPHLKKEKAPGIYRIALL
                     GDSMVEGLQVPIEDTFGRLLGKRLNEKLKQAPRPAITGVQVLNFGTSGYSTVQELLQL
                     EKQVLDYQPDLVIVGYNSRDIFENWAAPDEVLANVRPLALKIPGKELVVESGSVTCWM
                     RTPRAKILSALSFIRNHSRIWGVISYYENEASFHNPFYKLCLKFINQPGKTLRTIVQD
                     IGKDLGKAKPMQTTVPTTAPTNAPNVAAATQVATPAQKVPETTAKPEQSQDKPAPRVT
                     VDKGAEKFIALMKDTLEAIYLRMDRDLQARGGRLMVLVLPSRATLSPIGGMDKPLFNV
                     DYKGEGQIVKEICRKNHIACVDALTPATAFSLDKQCDLFYAAHLTRGGHKYVENLLAA
                     PVTQIVEEKRN"
BASE COUNT          690 a          745 c          884 g          642 t
ORIGIN      
        1 ccacccggcc gtttactgca aacaatagac tgaggggact gaccggactt tccagatcta
       61 gtaagaggtc aactttgtcg cctgccggta agtcggacaa agccaggttg gtttgcaccg
      121 gtctgagaag gctgtggcta accgtgtaga gttcgcgatc attgagtacg caaaagaaag
      181 tgcagaccgc agcgctgccg aagattgtga gagcggccag ggtcgctttg aaagggcgtg
      241 ccgtgccggc actggtgcgg cagagaaagc cggcaaaagc aagcacggaa agcgcacaga
      301 tcgatgccgt tatcaattcc agttcaccgt tacccagcac cgagggtgat gccatcaaga
      361 ccgccagggt tcgataactg cttagaatta gcaccaggag actactcaaa ctgagaagag
      421 ccggcagtgc cgtggttggt cccaggcgca ccaggcagtg agcggctaga atggctagaa
      481 tgggaatggc cgtgtagttg tagcgggaaa tcggctcgaa ggcgcagtag ctgaggtggg
      541 ccgctaggac caggagtgag agcagggcaa agcggcactg atttttgtct ctcgccgaaa
      601 gggtgagcat cagacctgac aaaccaagta gtaagagcag gcggtgtata aagtcttgct
      661 gggcggaatc gagcccgaag accttgtagc ggaattcatt ccaggaaccg gccaggagcc
      721 ggggcacctt tcttacttcc atcgaagcca ccgccaatgg ttcctgctta aatgacagga
      781 aaagtcgatt gatggcgtcg gccggcactg ttggaatatc tcgctggaaa ggataggctc
      841 gccagccgtc ccgttccaga tcggaaccga ggaagagatt aaaagccggt gaacgcgtta
      901 cggtcaaaag tggcttgccg gcaattgccc tggtaatgaa gagccagggg gcaaagacta
      961 aggccagccc aaggcagaag ggcagcgctt tcaggctcat ggttttcaac ccggagcgca
     1021 agccgttgcg cccggtcagc aagtaggcga gtgccactgc cggcggtagt aagtaaaaga
     1081 gcggcttaga cagcatgaga gcggccgtga gaaaacccag gaaaaccacc ggaccgtcca
     1141 ggggggctct acttctgcgc tctttcaaac ggtctacaaa taagagccag aggggcaaga
     1201 gcgcgctgca gaatatttcc gaataacatg actgcacatt caaacaggcg gacggataga
     1261 aggccagaaa aagggccgcc aggatagcgg tgagacgtcc gaaaaagcgt cttgccagta
     1321 agtaggtgca tacgcaactg aggctggaga caatactgat tatggtgccg agattccaaa
     1381 tgtgggcatt gaagtctttc gagccgggcg gtactccggc cagtagattg accacggcca
     1441 tataagtagt aatgacggga ccgtcattca taaagcggtc cgccagtttt gccgatgaca
     1501 taacctgggc gtcggtggcg cccacctgac cgagggccgc aagggccgct tccaaactat
     1561 tgagcttgcc gctcagtatg gagtgcgcga aattgagaag ttcgctgccg cctgagagaa
     1621 aatagaaggc atcaccttcc tgcaccacca ggtgttctat ggcgaataga taggtgagcc
     1681 tgagcgcaag ggccagaaga aaaatcagtg ccccgtcttt gatcaccttg tcggaagaca
     1741 tttcacccta atttcttttc tcttcgacga tttgagtcac cggcgcggcc agaaggtttt
     1801 ccacgtattt gtgaccgccc ctggtcaggt gcgccgcata aaacagatcg cactgcttgt
     1861 ccaagctgaa ggctgtagcc ggagttagag catctacgca ggcaatatgg tttttgcggc
     1921 agatctcttt cacaatttgc ccttcgcctt tgtagtcgac attgaagagg ggcttgtcca
     1981 taccgccaat cgggctgagg gtggcacggg agggcaagac cagcaccatg aggcgaccgc
     2041 cgcgggcttg caaatccctg tccatacgca aatagatggc ttccagcgtg tctttcatga
     2101 gagcgatgaa tttttccgcg cctttgtcca cagtcactct tggtgccggt ttatcctgtg
     2161 actgttccgg tttggcagtg gtttccggca ccttttgtgc gggggtggcg acctgtgtcg
     2221 ccgcagctac attcggtgcg tttgtcggtg ccgtggtcgg caccgtggtc tgcataggct
     2281 tggccttgcc gagatccttc cctatgtcct ggactatggt gcgcagggtt ttgcctggct
     2341 gatttataaa tttgaggcag agtttataga aagggttgtg aaaactggct tcgttctcgt
     2401 aataggagat cactccccag atgcggctgt ggttcctgat gaaactaagg gcgctgagaa
     2461 ttttggctct gggagtacgc atccagcagg ttaccgagcc tgattccacc accagctcct
     2521 tgccgggaat tttcagggcc agggggcgca cgttggcgag cacttcgtca ggggctgccc
     2581 agttttcaaa aatgtctcgg ctgttgtagc cgactatgac caggtcgggc tggtagtcca
     2641 gaacttgctt ctccagttgc aaaagctctt gcacggtgga atagccggag gtgccgaaat
     2701 tcaaaacttg cacgccggtg atagccggcc tcggcgcctg cttcagtttt tcattcaatc
     2761 ttttgccgag caggcgacca aaagtgtctt caattggcac ttgcaagcct tctaccatgg
     2821 aatcgcccag tagggcaatg cggtatatac caggcgcttt ttcctttttc aaatggggtt
     2881 cgcgcagacc ggcgccgtcg aagtacgagc gggagaagcc ctccttgcgc caggtgatgc
     2941 tcttattagt catgtgcttg g
//
LOCUS       DAOQNZ010000378         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17768_length_2956_cov_4.460876, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000378 DAOQNZ010000000
VERSION     DAOQNZ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17768_length_2956_cov_4.460876"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..512
                     /locus_tag="PKW73_07835"
     CDS             108..512
                     /locus_tag="PKW73_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73233.1"
                     /translation="MANGFDKVDQVEKAINSGDAKVADGVLKTIEDPTSQAIFKNIIT
                     QDCNAGNVLPPCTIDEGGIHFGGDHKMTMTDENGKLGMRDDQQSAFDKFKQGVSDTVD
                     GISKTIKEMNPEDWLGGLAGGAADKIRDNPHR"
     gene            841..1284
                     /locus_tag="PKW73_07840"
     CDS             841..1284
                     /locus_tag="PKW73_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73234.1"
                     /translation="MDGYERLNTAEKQINNGDVKGLRATLDTLPSQEDRQRFMRMMKD
                     RCVYNSTSPDCEIVGSDEGISQISYKGTHGLEIKAEETGKLKARLTNPTVMDLIDEYV
                     YEEKQKAAEEAKVAQKAKEECKKTDDSIAEKAFKAQYKQSTGEEL"
     gene            1516..2076
                     /locus_tag="PKW73_07845"
     CDS             1516..2076
                     /locus_tag="PKW73_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HNA73235.1"
                     /translation="MNTLADYTKEWLLESINEGASYQFHLFYGHKESPDGTVTDSCFS
                     QWFPADFEIEGINYKTAEHFMMAEKARLFQDQEALSDILACATPREAKALGRKVRGFD
                     DQVWHERRFGIVVRANQAKFSQNEGFKQRLLSTAPKVLVEASPRDRIWGIGMGQSNID
                     AHNPQKWRGRNLLGFALTKVRDEITP"
     gene            complement(2146..2568)
                     /locus_tag="PKW73_07850"
     CDS             complement(2146..2568)
                     /locus_tag="PKW73_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA73236.1"
                     /translation="MNNCLVFAFTSMLFAGFTSVIAKKGLTGISGDLGVTVRTCFVFL
                     FMLGFAWSAVPQKDWAELKPNNWLWLSASALTTTLSWIFYYRAIKLGEVSTVALIDKG
                     SVVVSVVLACWLLHEALTVPKLAGALLICAGLVVISKG"
BASE COUNT          922 a          716 c          719 g          599 t
ORIGIN      
        1 ctatcaaaag accgctgcaa tgagtcaatg acaaattgac caatgagcaa aactcgcttt
       61 actaacgccc agcatttgca agtggcgctt ttcttgagga acttaccatg gcaaacggat
      121 ttgataaagt agatcaagta gagaaggcaa tcaattcagg tgacgccaaa gttgccgacg
      181 gcgtgctaaa aaccattgaa gatccaactt ctcaggcgat tttcaaaaat ataattacgc
      241 aagattgcaa tgccggcaac gtgctaccgc cctgcaccat cgatgaaggc ggtatccact
      301 tcggcggcga tcacaaaatg accatgaccg atgaaaatgg aaaactcggc atgagagacg
      361 atcaacagag tgctttcgac aaatttaaac agggggtctc agatactgtc gacggcatct
      421 caaaaaccat caaagaaatg aatccggaag actggttggg tggcttagcc ggcggcgccg
      481 ccgacaaaat aagagacaat ccgcaccgct gacatcatcg gacaaaaaca agtttacatc
      541 gggagacggg tagagaacgt tgctgccgcc tgcttattta tcactgccgg cggcggcaac
      601 tctcttaaca gtcccgggat gtatagccaa tcgagcgaaa agatcggctc gcctcaggca
      661 actgcaacca cacttggtac tgcagttgtt cggatttagt cactgcaaaa aattatgatt
      721 taaaggtacc tggcaataca tgattctgac cggccgagtc gaaagactct gcgatcgcca
      781 ctcgacctgg aatctatctg gcatcaccgg caccaccagt gaactaaagc gaggaataag
      841 atggacggat atgaaagact aaatactgct gagaagcaaa tcaataatgg agacgtcaag
      901 ggactgagag ctaccctgga cactcttccc agccaagaag acagacagcg cttcatgcgc
      961 atgatgaaag atcgttgcgt gtacaattca acatctcccg actgcgagat agtaggctcc
     1021 gatgagggca tctcacagat tagttacaag ggtacccatg gccttgaaat aaaggcggaa
     1081 gaaactggaa aactgaaggc aaggctaaca aatcctactg tcatggacct tatagatgaa
     1141 tacgtctacg aggaaaagca gaaggctgca gaagaagcta aagtggcaca aaaggcgaaa
     1201 gaggaatgca agaaaacgga tgactcgatt gcagagaaag ctttcaaagc ccaatacaag
     1261 cagtccacag gtgaagaact ctagctgaaa tttcagaaat cgaagtatgc ggacgtaaca
     1321 ggacggagca cacgaaaagt cgctgagccc ctctgtcgca tcaatcttgc gacagagcca
     1381 ggttagagaa gcagtccggc tagagggaac ctccaaaaat ctcagactgc tgcggcaaag
     1441 aatgggaggc gctgacaaag ttggatgcta ccggctaaaa tatatgctca aatcagaaaa
     1501 ttgatggagc aagccgtgaa cacactagcc gactacacaa aagaatggct cctggagagt
     1561 atcaacgagg gcgcttccta tcagttccat ctattctacg ggcacaaaga aagccctgac
     1621 ggcaccgtca ctgatagctg tttcagtcag tggtttccgg cagattttga aattgaaggc
     1681 atcaactaca aaactgccga acactttatg atggcggaaa aggcgcgact ctttcaggac
     1741 caggaggcgc tttccgacat tcttgcttgt gctactccga gagaggcaaa ggcacttggt
     1801 cgaaaagtcc gcggattcga tgaccaggtc tggcatgaaa gacgctttgg catcgtcgtg
     1861 cgagccaatc aagcaaagtt cagccagaat gaaggtttca agcaaaggct cctatcgacg
     1921 gcaccgaaag tgctggtaga agccagtccc cgagacagaa tctggggtat cggcatggga
     1981 caaagcaata ttgatgccca taacccgcaa aaatggcgag gcaggaattt gcttggcttt
     2041 gctctcacga aggtaagaga tgagataaca ccataatcga agactctctc aatagaacag
     2101 aaaggtctgc acggctaata tagcaaccta tctaagcaga aagtctcagc ccttcgaaat
     2161 tacaaccagt ccggcacaga tcaagagtgc accggcgagt ttcggcacgg tcagggcttc
     2221 atgcaaaagc cagcaggcta gcacaaccga cacaaccacg ctgcccttat caataagcgc
     2281 cacggtagac acttcaccca attttatggc tcgatagtaa aaaatccatg acagcgttgt
     2341 tgtcagcgct gaagccgata gccagagcca attattcggt ttaagctcag cccaatcttt
     2401 ttgaggcacg gcagaccagg caaagccaag catgaacaaa aacacgaaac aggttctcac
     2461 ggtcacaccc agatcaccgg agatgcctgt cagtcccttc tttgcgataa cggaggtaaa
     2521 cccggcaaat aacatggaag taaatgcaaa gaccaaacag ttattcattt cttctcttca
     2581 gctccgtttt tcggccggcg gttcccataa ggggcagaga accataacac atattagctc
     2641 gactgtgcgc ggttccccgc atagatgatc aacggagtca tcgcctcttg tcggaacctt
     2701 tcaatagtgc gccccttgaa tcccgttcgc aggcgcggga caattacctc acaaaaatga
     2761 cgcagaagag cggtagcaat ttacatgaag cgttttcaga aatcactgct tgcagcagtt
     2821 tatttaacag ctaccggctg cgctcagatg gcattcggtg gcgaaattca taagactccc
     2881 attgagatat tgaaggcttt accgacggaa tccggttcgg cacaagacga gaagccaatt
     2941 ctaggcggaa cattta
//
LOCUS       DAOQNZ010000379         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17782_length_2955_cov_3.276897, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000379 DAOQNZ010000000
VERSION     DAOQNZ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17782_length_2955_cov_3.276897"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKW73_07855"
     CDS             complement(<1..339)
                     /locus_tag="PKW73_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNA73237.1"
                     /translation="MATKSETYMWDRERETISRSDLKNLQLARLKQVLERVWHNVPTL
                     KKRFQEAGIDETTISNLQDVEALKDFPFMKKSDLRDNYPFGLFASPMKEVNRLHCSSG
                     TKGKPTVVGYT"
     gene            1572..2606
                     /locus_tag="PKW73_07860"
     CDS             1572..2606
                     /locus_tag="PKW73_07860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNA73238.1"
                     /translation="MLFGGLLFGGADVVYRHFYPGRVALENFLKPAGFFTREGSKALY
                     IGYLVALVHLGWIVLYYLLGEKFSFWCPSGIDSVEVLSATVPFYSAVSLGLHAASQEE
                     TVARIVGLSLAWKATGRFWLANLFQAVIWAFMHSSYPQQPCFARGVELTVVGLFYGFV
                     LRRYGLLPCFIGHYLLDAFLDVKPLLSVGPGQLWLWLSSFIPLVPFLLLFFLSLFVTR
                     LRADSPQVDGETSVHETGDAALTNEAVSKSFGTTVATATPVGDSVQDVYVYNGFSRRF
                     RLIAGITSLVLLAITFFIHLPTCGDDARCQIGREQAVTRARLILQKAGINVKNHFNLG
                     TSAGGWLNAH"
     gene            complement(2601..2948)
                     /locus_tag="PKW73_07865"
                     /pseudo
     CDS             complement(2601..2948)
                     /locus_tag="PKW73_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
BASE COUNT          575 a          759 c          813 g          808 t
ORIGIN      
        1 ggtgtaaccc accaccgtcg gtttgccttt ggtgccggag gaacagtgca gtcgattgac
       61 ttctttcatg gggctggcga agagaccaaa aggatagttg tccctcaggt cggacttctt
      121 catgaagggg aagtctttaa gcgcctcgac gtcttgcaag ttgcttatgg ttgtttcatc
      181 aataccggct tcttgaaagc gttttttgag agttggcaca ttgtgccaga ctctttccaa
      241 tacctgcttc agtcttgcca gttgcaagtt cttcaggtct gagcgggaga tcgtctcgcg
      301 ctccctgtcc cacatataag tttcggactt tgtagccacg gcgaaatcgg tgcctcacaa
      361 gcacttgtaa caaaacgtcc atattacccc ctcggcatct tgtttgtcga taatcgtcgc
      421 cggggctagc tgatggagtc cgctaccggc gccacttctc ggggcgttgg cggggactga
      481 gttgggaaat ttcaggtttt gttaatgtct gtgagcctgg tattatcaaa tcgaacagtc
      541 aaagaatcaa cgtgaaagag atggaaaccc cggggcctga agaaactggg agcaagcggg
      601 acagggttgc tctggttttg ctcattctgg ctcttctggg tgggctttta tacgcccggt
      661 tgcaaagcaa tgtattcccc acggcttccc tatcgctgcc cctcagtcgc agtgaaattg
      721 tgcagcgagc tcgcagtttt gccggtcagc aaggttacga tggacgcctc tggccccttg
      781 ataaagcgga tggggccagg aaaaaagaga ttgagtccat aactttcacc agcttcgatg
      841 aggctaaaac ctttctcgaa tacgaactcg gtctttctca ggccggagcc atcatgcgtg
      901 acaaggtgcc ggtctgggcc tggtctgtgc gcttttgccg ggagtttacc attgagcaat
      961 gccgggtctg gctttcgccg gacggcgccc tggtgggtta tttgcgcacc ctggaggaag
     1021 agcggcgtct gcccagtctg agtcatcaag aggcgctcta ccttgcttcc gattttctca
     1081 agctggaagg gcgagtgcag gaaggcacgc tcaagttggt gcgagacgag accaatgcca
     1141 gggtgcatcg aaccgatcat tcttttactt ttgaggataa gcgggaggat tttcacggcg
     1201 gcaagctgag gcatttcgtg gaagtctcag gcaatatggt caccggttac aaccgctact
     1261 tgttcgtgcc cgacagttgg agccgcaagt acaacaacat gcggtcatac aatgaactct
     1321 tgcagcgcat tgccaatatt ttctacagca ctctccagat aatctctgct ctggttgtgc
     1381 cctgggccct ttctcgcaag cagatgcgct ggcggttcgc cgtattcggc ggcttgacca
     1441 tggccctgac gggctttctt gactgcgtca atgacttcga atcgattcag gccgcttacg
     1501 atactacttc ctcttataac gattacttgc tcgcctattt tggtcgctcc ggggcctcgg
     1561 ctctcgggtc gatgcttttt ggcggactgc ttttcggcgg tgccgatgtg gtttacaggc
     1621 acttttaccc cggccgggtg gccctggaga atttcctcaa accggccggt tttttcacca
     1681 gggaaggttc aaaggcgctc tacataggct acctggtggc cctggtgcac ctgggctgga
     1741 ttgttcttta ttacctgctg ggtgaaaaat tcagtttctg gtgcccgagc ggcatagaca
     1801 gtgtggaggt gttgagcgct acggtgccgt tttattcggc cgtttctctt gggttgcatg
     1861 ctgcttccca ggaagaaaca gtggcaagaa ttgtgggttt gtccctggcc tggaaagcca
     1921 ccggtcgctt ctggctggct aatctttttc aggcggtcat ctgggccttt atgcacagtt
     1981 cttatcccca gcaaccttgc tttgcccgcg gtgtggagct gaccgtagtc ggtctttttt
     2041 acggctttgt tttgcggcgc tacgggcttc tgccttgctt catcgggcac tacctcttag
     2101 atgcctttct tgatgtcaaa ccgctcctct cggttggccc gggtcaactc tggctctggc
     2161 tgtcttcctt cattcctctt gtgccgtttc tgctgctctt ctttctttca ctcttcgtca
     2221 ccaggctgcg ggctgactcg ccgcaggtgg atggagaaac atcggtgcat gagaccggag
     2281 acgctgcact caccaatgag gccgtaagca agtcattcgg cactactgtc gcgacggcaa
     2341 cgcctgtagg cgacagtgtg caagatgtct acgtgtacaa tggattctcc aggcggtttc
     2401 gattgatcgc cggcattacc tcgctggttc tcttggccat tactttcttc attcatttgc
     2461 ccacttgtgg tgatgatgcc cgttgccaga tcgggcggga acaggcggtg actcgggcgc
     2521 ggctcatttt gcagaaagcc ggcatcaatg tcaaaaatca cttcaatttg gggacgtccg
     2581 cggggggctg gcttaatgcc cactaaatca aattgaccca gtaatttgtt gttggcggcc
     2641 atttctcgtt ctccttggaa aacccgaatg gtcacggcag tttgattgtc ttctgctgtg
     2701 gagaaaacct gggatttttt tgtaggaatg gttgtgttac ggtcaataag gcgggtgaaa
     2761 acgccgccca tagtttcaat acccaaggac aaaggtgtga catccaacaa caagagatct
     2821 ttgttgcctt ccccttgaag aacaccagct tgaacggcgg caccaagggc cacaacttca
     2881 tcagggttca caccacggtg gggttcttta ccaaagaatt ctttcacggt ttcaatgatt
     2941 ttgggcatgc gggtc
//
LOCUS       DAOQNZ010000380         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17785_length_2954_cov_4.153156, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000380 DAOQNZ010000000
VERSION     DAOQNZ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17785_length_2954_cov_4.153156"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PKW73_07870"
     CDS             complement(<1..802)
                     /locus_tag="PKW73_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73239.1"
                     /translation="MVHSHNQPNNSDNSEELSAKTQADEELLKANERLAPEAREPGSH
                     KLSGNLQVEASGSGLKEESEKRAFLTRKIETGGLSRHIEPEKKDEIRTRKEFTLAGND
                     SDLNPSANPGLFFPIGLRQHFQLRKSEFEGEKSELRLNNLKPQITDLILQTHITDDDK
                     LTSAERAGLARARENLTNSLSPTDALSNALHLARLYQHLRYIEEAKKSTLFALGIDPD
                     NQLGKQLFKELERVHPTDLGVKSAVPMMSPLSRNRLKTRIINLSGGAVT"
     gene            867..1400
                     /locus_tag="PKW73_07875"
     CDS             867..1400
                     /locus_tag="PKW73_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73240.1"
                     /translation="MTKVLYREFFALVALLWSACPLVLAEEPQLKPAIKNSAAGGAQP
                     IALSASHVRKDVGAAKKMAALRWFDKMALADPQLVESITAYSSSASILARHPHLDKIA
                     EADPYLCRRLTRWRGAARVLARNPKAYKVVARDPEGIYRAIKNDRSYAKLLSKNPYFN
                     QMVVENPELGRVLSRYM"
     gene            1400..2194
                     /locus_tag="PKW73_07880"
     CDS             1400..2194
                     /locus_tag="PKW73_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA73241.1"
                     /translation="MAEAFSQSLWAPLLIGLATGLLSGTFGVGGGIVATPLLRLCLGL
                     SPQVAVGTTLAVILPTSLTGVINYYRKSFVDTRLALRLALPSALAGYVGARLTDAVSG
                     SFLMVAFALLIALAGFDLLTGFSEKLKKKAAGETYESPHWSKVLPVGFLAGFLSGFFG
                     VGGGFVMIPIFIAYFSMPLKLALGTSLLTVAFIAVPGILTHFMLNHVRLDLTLLLVSG
                     AVPGSYLGSRLAIIIKDNLLRKAFAFIMLLIAGQMVVSEIPKLGTH"
     gene            2196..2801
                     /locus_tag="PKW73_07885"
     CDS             2196..2801
                     /locus_tag="PKW73_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73242.1"
                     /translation="MLHIRLIEMEAEKLAREEAERALKVGEKQLPHECELIKCHIEEE
                     VGLKLAQSCASDFRGWLTYRRLNGDLVIIRGRAFQEASSFMFGAGKWKAGLDVPFGLD
                     TVRERQRGFDVSPPQAVDHLMVNFGMGKCRRVFLWYGENIAPYRLIFEEELQEIQDLP
                     VGESPQKNLMEAMSEGMGWILNKPRELTLPEYIKQLKPKPC"
BASE COUNT          586 a          724 c          816 g          828 t
ORIGIN      
        1 ccgtcactgc gccgccgctc aagttgataa tgcgggtttt cagacggttt ctggaaagcg
       61 ggctcatcat tggaaccgcg gattttacgc ctaaatcagt tggatgcact ctctccaatt
      121 ccttaaatag ctgcttgccg agctggttgt cagggtctat gcccagggca aagagggtag
      181 acttctttgc ttcctcaata tagcgtaaat gttggtagag acgtgctaga tgaagcgcat
      241 ttgaaagagc gtcggtgggt gaaagggaat tagtgagatt ttctctggcc ctggcaagac
      301 cggccctttc agcgcttgtg agcttgtcgt cgtcggttat atgggtctga agaatcaaat
      361 cggtaatttg aggtttcaaa ttatttagtc tcagttcgct cttttcacct tcaaattccg
      421 attttctaag ctgaaaatgc tgcctcaaac cgatggggaa aaagagaccg ggattggcgg
      481 aaggattcag gtcgctgtca ttgccggcca gggtgaactc tttgcgggtc ctgatttcat
      541 ctttcttttc cggttcgatg tggcgactga gtccgccggt ttcaattttg cgagtgagaa
      601 aggctctctt ttcgctttcc tctttcagac cactgccgct ggcctccacc tgcaagttgc
      661 cggagagctt gtgcgaaccg ggctctctcg cttcgggcgc caggcgttca ttggccttga
      721 gaagctcctc gtccgcctgt gttttagcgg acagctcttc ggagttatcg ctattgtttg
      781 gctggttgtg ggaatggacc atgggttttc aagaatcgct ttctacatag gcattataag
      841 tggcggagcg gtgctaaaat agccccatga ctaaagtctt gtatcgtgag ttttttgccc
      901 ttgtggcgct tctctggtcg gcctgtcctc tggttttagc ggaagagccg cagttgaagc
      961 cggctatcaa aaattctgcc gctggtggcg cccaaccgat cgctctcagc gccagtcatg
     1021 ttcgtaaaga tgtgggtgcc gctaagaaaa tggcggcttt gcgatggttc gacaaaatgg
     1081 cgctggctga tccgcaactt gtggagtcaa tcaccgccta ttcatcttct gcctcaatac
     1141 tggctcgtca tcctcattta gataaaattg ccgaagccga tccttatctc tgccgccgat
     1201 tgactcggtg gcgtggtgcc gcccgagttc tggccaggaa cccgaaggcc tacaaagtgg
     1261 tggcaagaga tcccgagggt atatatcggg ccataaaaaa tgacagatct tatgccaagt
     1321 tgctctctaa aaacccctat ttcaatcaga tggtggtaga aaacccggag ctgggcagag
     1381 tattgtcgcg ctacatgtga tggcggaagc attcagccag agcctctggg ctcccctttt
     1441 gattggtctg gccactggtc ttctgtcggg tacctttggt gtcggcggcg gtatcgtggc
     1501 tacgcccctt ctgaggcttt gtctgggact ttccccccag gtggcggtgg gaacaaccct
     1561 ggccgtgatt ttgcccacct ccctcactgg tgtaatcaac tattatcgta aaagcttcgt
     1621 ggacacgcga ctggctttac ggctggcttt gccttcggcc ctggccggtt atgtgggcgc
     1681 ccgcttgacc gatgccgtaa gcggctcttt tctgatggtg gcttttgcct tgcttatcgc
     1741 cctggccggc tttgatcttt tgacgggctt tagcgagaaa ctgaagaaga aggccgccgg
     1801 cgagacctac gaaagcccgc actggtcaaa ggttttgccg gtgggatttt tggccggttt
     1861 cctctccggc ttttttggtg tgggcggggg atttgtcatg attcctattt ttattgctta
     1921 tttttccatg cccctgaaac tggcccttgg tacttcgctt ttgaccgtgg cttttattgc
     1981 cgtgcccggc atcctcactc actttatgct caatcatgtg cgtcttgatc tgaccctgct
     2041 tctggtctcg ggggcggtgc caggctccta tcttggcagt cgcctggcta taattattaa
     2101 agacaatcta ctgcgcaaag cttttgcctt tattatgctt ttgatcgccg ggcagatggt
     2161 tgttagcgaa attccaaaac tggggacaca ttgacttgct tcatattcga ctgattgaga
     2221 tggaagcgga aaagctagcc cgagaggagg ctgaacgtgc cctcaaggtc ggcgagaagc
     2281 aacttccaca tgaatgcgag cttattaagt gtcacatcga agaagaggtg ggattgaagt
     2341 tggctcagtc ctgcgcctca gacttccgcg gttggctgac ctaccggcgt ctcaacggtg
     2401 atctggtgat cattcgcggg cgagcctttc aggaagcttc ctctttcatg ttcggcgctg
     2461 gtaagtggaa agccggtctg gatgtaccct tcggtcttga taccgtgcgg gagcgccagc
     2521 gcggctttga tgtctcaccg ccccaggctg ttgatcacct catggtcaat ttcggcatgg
     2581 gcaagtgccg cagggttttt ctctggtacg gcgaaaatat tgcgccttac agactgattt
     2641 ttgaggaaga gctgcaggag atacaggatc ttcccgtggg cgaaagcccc cagaagaatc
     2701 ttatggaagc catgagcgaa ggcatgggtt ggattttgaa caagcccagg gaattgaccc
     2761 tgcccgagta cataaagcaa ctgaagccga agccctgcta agtcttgatt ttgttccggt
     2821 ttatgggtga taagctgatc tgagccttaa ttcaggtttc aaagctgttt ctcaattatc
     2881 atgctcccat gggtggaaag ttctttagct tcatgagcct caagggtgcc ggtagggcag
     2941 tgtttcctgc cagt
//
LOCUS       DAOQNZ010000381         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17949_length_2940_cov_3.725477, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000381 DAOQNZ010000000
VERSION     DAOQNZ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17949_length_2940_cov_3.725477"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKW73_07890"
     CDS             complement(<1..458)
                     /locus_tag="PKW73_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA73243.1"
                     /translation="MAVVQEKELLCKEFLSRPKQLLIDGKWVNSVSGKTFETLNPATE
                     EVLCRVAEGGKEDVDKAVKAARRAFETGPWRKMSTAERARIMLRLADLIEKHADELAQ
                     IETLDNGKPINESRYVDVPQTVETFRYYAGWATKLEGETINANTNFFTYT"
     gene            complement(592..1443)
                     /locus_tag="PKW73_07895"
     CDS             complement(592..1443)
                     /locus_tag="PKW73_07895"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNA73244.1"
                     /translation="MVNAFSKTALESEPDLAGRQDHNGITFADVNAFQPAVLKGDQQN
                     SLQLVAAGGEVPKTKTVYVDIGHCTSGERNPLGVGGSDQGFAGPGYSECDINKAVGKE
                     LMKELKNMGLYVVPTWDVNNPPPPVPKSQDLDRRIDTVNRDVAKYGDDAVYVSIHHDS
                     DTTKQGGQCVFYAATKREESQVLARSIQANAWQVREREGVKDCINPDTATGKGYLDGL
                     RRTNAIGVLIEGANVQNPRDRALMPQSRFQQLEAKGIAQGIFNYFNLRPGQERPYSSA
                     IRQRFGL"
     gene            complement(1536..>2940)
                     /gene="ftsH"
                     /locus_tag="PKW73_07900"
     CDS             complement(1536..>2940)
                     /gene="ftsH"
                     /locus_tag="PKW73_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNA73245.1"
                     /translation="GVLLVGSPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGA
                     SRVRDLFDQAKKQAPCIVFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFEGS
                     TGIIVVAATNRPDILDSALLRPGRFDRQVVIDRPDVSGREQILNVHGKGKPLAKDVDL
                     KILARRTPGFTGADLSNLINEAALIAARADKREIEMTDLELAIDKVIAGPEKRSRLIP
                     PKEKEMTAYHEVGHALMNVLLPHAHPLHKVTIIPRGFALGLTMAVPEEDVLSMTKAMI
                     VDQIGVCLGGRVAEEVVYGDVTTGAQNDLEKSTGLARRMVTEFGMSERLGPMTFGKRH
                     EQVFLGRDFGHERDYGENIATAIDEEVSEIISGQYARVKELLLTHRPHMDALVKALLE
                     KETLDKKEVDAILEEVNRRLALGEDPTSPSSSDNDRQEPPSAVIVTREEEKQPVMKES
                     QNDSGGDASPGLTPKFA"
BASE COUNT          639 a          815 c          794 g          692 t
ORIGIN      
        1 aaggtgtagg tgaagaaatt ggtgttggca ttgatggttt cgccttccag tttggtcgcc
       61 caaccggcgt agtaccggaa ggtctcaact gtctgcggaa catccacgta gcgagattca
      121 ttaatcggct taccattgtc gagggtctca atctgagcca actcgtcggc atgcttttca
      181 atcaggtcgg ccaatctaag catgatgcgc gcccgctcgg cggtgctcat cttgcgccag
      241 ggaccggttt caaaggctct acgagcagct ttgacggcct tatccacatc ttccttgccg
      301 ccttcggcaa cacggcaaag tacctcttcc gttgccggat tcagtgtctc aaaagttttt
      361 cccgatactg aattaaccca cttcccatca atgagaagct gtttggggcg ggaaaggaat
      421 tccttgcaaa gtagttcttt ttcttgaacg acggccacgc taagaccctc cgaaactgac
      481 agttgtacgg actctggaca actaactatt agctggctag attatcacga accgttaagt
      541 aactgtctga ctcaagaaaa ctcttaagta tggcctaatt atttacaagc ctcaaagacc
      601 gaagcgctgg cgaatagcgg acgaataagg ccgctcctgc cccggacgga gattgaagta
      661 attgaaaatg ccctgagcga taccctttgc ctctagctgt tgaaaacgac tctggggcat
      721 aagggcccgg tcgcgaggat tttgaacgtt ggcgccttca atgagcactc caatagcgtt
      781 agtgcgacgc aagccatcca gataaccctt gccggtggcc gtatcggggt tgatgcaatc
      841 cttcacccct tcacgttccc gcacttgcca ggcgtttgct tgaatggagc gggccagcac
      901 ctggctttcc tcacgcttgg tggcggcgta aaagacacac tgcccacctt gcttggtggt
      961 atcggaatcg tgatgaatac tgacatagac agcatcatcg ccatatttgg ctacatcccg
     1021 attgacagta tcgatacggc gatcgagatc ctgactttta ggcaccggcg gcggcggatt
     1081 attcacatcc caggtaggaa caacataaag tcccatgttc ttgagctctt tcatgagctc
     1141 tttgcctacg gccttgttga tatcgcattc ggaatagccg ggaccggcaa agccctgatc
     1201 gctgccgccc acccctaaag gattccgctc gccgctagtg caatgaccga tatcgacgta
     1261 gacggttttg gtcttcggca cctcaccacc ggcagccaca agttgcaggg agttttgctg
     1321 gtcgcctttc aaaaccgccg gctgaaaagc atttacgtca gcaaaagtaa tgccgttatg
     1381 gtcttgccga ccggcaagat caggctccga ttccagcgcc gttttggaaa aagcgtttac
     1441 caaagcaaca acctctaagt tttgggggtg aacccagttt acttagaggt tgtgaaagtt
     1501 tcgtaaaagt gggccagtcc gcaattacaa caggatcagg cgaacttggg agtcaagccg
     1561 gggctggcgt cgccgccgct gtcgttctga ctctctttca taaccggttg cttctcttct
     1621 tcgcgggtga caatcaccgc cgaaggaggc tcttgccgat cattgtcgga agaactgggg
     1681 cttgtcggat cttcaccgag ggcaaggcga cgattcacct cttccagaat ggcgtcgact
     1741 tcttttttgt ccagggtttc cttctctaag agtgccttca caagcgcatc catgtggggg
     1801 cgatgggtca ggagtaactc cttcacccgg gcgtactgtc cggaaataat ttccgaaact
     1861 tcttcgtcga tggcagtggc gatgttctca ccgtaatcac gctcatgacc gaaatcacga
     1921 ccgaggaaga cctgttcatg gcgcttgccg aaagtcatcg gtcccagtct ttcgctcatg
     1981 ccgaactcgg taaccatgcg ccgggccagc ccggtgctct tctcgaggtc gttctgagcg
     2041 cccgtggtga catcaccgta aactacttcc tcagccactc taccgcccag acatacacca
     2101 atctgatcaa cgatcatggc cttggtcatg gagagcacat cttcctccgg cacagccatg
     2161 gtcagaccaa gggcaaagcc ccggggaatg attgtcactt tgtgcagggg atgggcgtgg
     2221 gggaggagga cattcatgag cgcatgaccg acttcatggt aggcggtcat ctctttctct
     2281 ttcggaggga taaggcggga gcgcttctcc ggtccggcaa tgaccttgtc gatggccagt
     2341 tccagatcgg tcatttcaat ttcacgcttg tcggcccggg cggcaatcaa agcagcttcg
     2401 ttgatgaggt tgctcaggtc ggcgccggtg aagccggggg tgcgccgggc caaaatcttc
     2461 aggtctacat ctttggccag gggtttgccc ttgccgtgca cgttgagaat ttgttctcgc
     2521 ccgctcacat cgggtctgtc aatcaccacc tggcggtcga agcgacccgg acgcaggagg
     2581 gcggagtcga gaatgtcggg gcggttggtg gcagctacaa cgatgatgcc ggtgctgcct
     2641 tcaaaaccgt ccatctccac caggagctgg ttgagggtct gttctctttc atcatgaccg
     2701 ccgccgaggc cggcgccgcg ctgtctgcct acggcatcga tttcatctac gaagacgatg
     2761 cagggtgcct gtttcttggc ctggtcgaag aggtctctca cacgggaagc accaacaccg
     2821 acaaacattt ctacgaaatc agagccggag atgctaaaga agggtacgcc tgcctccccg
     2881 gcaacggctt tggcaagcag ggtcttgccg gtaccaggcg aaccgaccag gagcacgccc
//
LOCUS       DAOQNZ010000382         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17969_length_2939_cov_3.476768, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000382 DAOQNZ010000000
VERSION     DAOQNZ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17969_length_2939_cov_3.476768"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="PKW73_07905"
     CDS             <1..1862
                     /locus_tag="PKW73_07905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA73246.1"
                     /translation="DYQLESLNESDHYGRLLSRIGCNKRVLELGSSTGYLSQAMTERG
                     CSVTGVEIDPDAAEVARARGFEVHVLDLDFIDLSKHFAGRSFDAVLLADVLEHLRSPE
                     QTLKQLATLLAPDGLIVASIPHGTHGDIRMAALLGRLPFRPMGLLDHTHVRFFTRSLV
                     EKLFVESGYDIVQLERNRWSTGYTEVSFEAPRTLPPDLAGLKHLLDCDPEADTYQFIV
                     QAKLSQPLTENLPAVEVAVIDTPLQRADDLYHQALKLMKYDQSKLRFWFCSGTAAEDK
                     IEIADRPGKETPKLADSDFRSFRFLDRKGVDTIPEHASQCRLQVADSQWGSPATNNIG
                     KILKRIALGTNAPYIFVLTADTLPAATCLQKLVTEAEQLDCGKERILLTARAEIALAC
                     EEEGGSACLTGCKDSDGNLSWHEFAGMLIPRELLLTADLSAPGRSAPQEKGTAASPDG
                     ATISTGPGTPCALSQAQGDYAPKENLKEFRKREYGLPTDGDSLLDSSFMTNLAQAQDF
                     CFRLWQCGARVKEGSGTYFANGPRLPQGSAEERFFDGLRLRRRWGTNRQFLSFARYII
                     GRAWREGGAWQSLKFLNHTLAAALLEPPSNLPQTSPAKELVGFHGPYARHTGA"
     gene            complement(1881..>2939)
                     /locus_tag="PKW73_07910"
     CDS             complement(1881..>2939)
                     /locus_tag="PKW73_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA73247.1"
                     /translation="EIVSTALAGREVTEVVEGTKRFGLLVRFPMDFRDTQEDIESILV
                     DTPNGGKVPLKQLAEITNTSGTVMINREDGQRRTAVLSNVRGRDLGSFVEEVQARVNK
                     EVEIPRGYRIVWGGQFENQQRAMARLALVVPVVLLLIFILLFASFNSLRNAGLIMLNV
                     PFAIIGGIIALLISRQPISVPAIIGFIALFGVAVQNGVIMVSYIMQLEKKGVPVLEAT
                     IEGVKVRMRPVIMTALVAMVGFLPLIFSHGTGAEVQKPLATVVLGGLFTSTILTLIVL
                     PTLYTLINKDPDVAPTAGRNGGYLPPPLESSGHVSSTAGGGATAKDGNGSGIAASSSG
                     GKESLVAEHEGDSVSPQQ"
BASE COUNT          676 a          884 c          794 g          585 t
ORIGIN      
        1 atgactacca gctagagagt ctcaacgaaa gcgaccacta cgggcggctt ttaagccgca
       61 taggctgcaa taagagggtg ctggaattgg gctccagcac cggctatctc tcccaagcca
      121 tgacggagcg cggttgtagc gtcaccggtg tagaaataga cccggatgca gcggaagtag
      181 ccagggcacg cggctttgaa gtgcatgtct tagacctgga cttcatcgat ttgtccaaac
      241 actttgccgg acgcagcttc gatgccgtgc ttctggctga tgtgctcgaa catctgcgca
      301 gcccggaaca aaccctcaag caactagcga ccctactggc accggatggt ctgatcgtgg
      361 catccatccc ccacggcact cacggcgaca tcagaatggc agccctcctt ggtcgactgc
      421 ctttccgccc catgggcctg ctggatcaca cccatgtacg ctttttcaca cgctctctgg
      481 tggagaaact cttcgtagag agcggttacg acattgtgca attggagcga aatcgctgga
      541 gtaccggcta cacggaagtc agcttcgaag cgccccgaac tctgccgccc gacctggccg
      601 gtctcaaaca cctgctcgat tgcgacccgg aagccgacac ctaccagttc atcgtgcagg
      661 caaaattgtc ccagcccctg actgagaact tgccggcggt ggaagtggct gtaatcgaca
      721 cgcccttaca aagggccgat gacctctatc accaggccct caaacttatg aagtatgacc
      781 agagtaaact gcgattctgg ttttgctccg gcactgccgc cgaggacaaa attgaaattg
      841 ccgaccggcc tggtaaagag acccctaaat tggccgacag tgacttccgc agcttccgtt
      901 ttctcgatcg aaaaggagtt gataccattc cggaacatgc cagccaatgt cggctgcaag
      961 ttgccgacag tcagtggggc agccccgcta cgaacaacat aggcaaaatt ctcaaacgca
     1021 ttgccctggg cacaaatgct ccttacattt ttgtgctgac ggccgatacg ctgccggcag
     1081 ccacttgctt gcagaagctg gtaacagaag ctgagcagct cgattgcggc aaggagcgta
     1141 ttctgctaac cgccagagcc gagatcgccc tggcctgcga ggaggaaggt ggcagcgcct
     1201 gcctcaccgg ctgcaaagac agtgacggga atctgtcctg gcatgaattt gcaggcatgc
     1261 tcattccacg agaactgctg ctcaccgctg acctgagcgc cccaggccgg tctgccccgc
     1321 aagagaaagg cacagccgcc agcccggatg gggcaacaat ttcaaccggg ccgggaaccc
     1381 cttgtgccct ctcgcaggcg caaggagatt atgctcccaa agaaaacctg aaagaattca
     1441 ggaagcgcga atacggtctt ccgacagatg gcgacagcct tttggattcc agcttcatga
     1501 ccaatctggc tcaggctcag gacttttgct ttcgactctg gcaatgcggc gcccgggtca
     1561 aggaaggctc cggcacctat ttcgccaatg gtccgcgtct acctcaggga agtgcggaag
     1621 agcggttctt tgacggcttg cgcctgcgca gacgctgggg caccaatcgc cagttcctct
     1681 ccttcgctcg ctatatcatc ggtagagcct ggcgagaagg cggagcctgg caaagtctga
     1741 agttcttaaa tcacacgctg gcagccgccc tactggagcc gccttcgaat ctaccccaga
     1801 cgagcccggc gaaagaactg gtcggttttc acggacctta cgcccgccac acaggcgcct
     1861 gaagaagtca cttaatcggc ttactgttgc ggcgagacgc tgtcgccttc gtgctcggca
     1921 accagacttt ctttgccgcc gctagaagat gcggcaatgc ctgagccgtt gccgtcctta
     1981 gcagtagcac cgccaccggc tgtactgctc acgtgtccgg acgattcaag gggaggcggc
     2041 agatatccac cgttcctacc ggctgtgggt gccacgtccg gatctttgtt aatgagcgta
     2101 tagagcgtcg gcaaaactat cagagttaag atggtggaag taaacagtcc acctagaacc
     2161 acagtggcca ggggcttctg cacttcggcg ccagtgccgt gggagaaaat caagggcagg
     2221 aaacctacca tggccacaag ggccgtcatg ataacggggc gcattcttac tttcaccccc
     2281 tcaatggtag cctcaaggac aggcacgcct ttcttctcca gttgcatgat atagctgacc
     2341 atgatgacgc cgttttgcac cgccactcca aagagagcaa tgaagccgat gatagccggc
     2401 accgaaatgg gctgacggga gataagcagg gcgatgatgc cgccgataat ggcgaagggc
     2461 acgttcaaca taatcagacc ggcattacgc aagctgttga aggaggcaaa gagcaaaata
     2521 aagatgagca ataagaccac cggcaccacc agcgccaggc gagccatggc ccgctgctgg
     2581 ttctcgaact gcccgcccca gactatgcgg tatccccgcg gtatctcgac ttccttattc
     2641 accctggcct gcacttcttc cacaaagcta cccaggtcgc gaccgcgcac gttggagaga
     2701 acagcggtac ggcgctgtcc gtcttctctg ttgatcatga cggtaccact cgtattggtt
     2761 atctccgcca actgcttgag gggcactttg ccaccattgg gggtatcaac caggatagac
     2821 tcgatgtctt cctgagtatc cctaaaatcc atggggaagc gcaccaggag cccgaagcgc
     2881 ttcgtaccct ccaccacttc cgtcacttcc cgcccggcca gcgccgtaga aacaatctc
//
LOCUS       DAOQNZ010000383         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_17971_length_2939_cov_3.398752, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000383 DAOQNZ010000000
VERSION     DAOQNZ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17971_length_2939_cov_3.398752"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKW73_07915"
     CDS             <1..381
                     /locus_tag="PKW73_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73248.1"
                     /translation="SLTLENLAEIYWRRRKYDKAEPLCKQILQIAESLLGPEHSDVGL
                     AANNLAILCERQGKHSDAAILYQQALTIAENILGNSHPDTEILRESHARARFQALKQV
                     EQKLEESHKGSRWSKSGWWKAYQQ"
     gene            complement(402..1064)
                     /locus_tag="PKW73_07920"
     CDS             complement(402..1064)
                     /locus_tag="PKW73_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73249.1"
                     /translation="MSGKNNVVIIARSISKPERHVGSAQRSYIAELSYQVVKGQWTGL
                     LQGSRKQYVKRLCELHLARATRTLVQDLLRIYKTDDLLELIDLVAYQTEVDYASLAAF
                     ILDWKMPTVATLEKIARRSKLKIALDVVPLKELPIAVEPPVYAFGTGDLAGKLGEVSR
                     FYLGYFIPRKDLRKASFLTGSQLEAALSGRCHKLLTVQCLLHVRRLKLRLRFVEIDGG
                     LY"
     gene            1487..2032
                     /locus_tag="PKW73_07925"
     CDS             1487..2032
                     /locus_tag="PKW73_07925"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNA73250.1"
                     /translation="MTRKNTIIDCGDFTIRSLVKADADSLSANANDYDIWLNLRDRFP
                     HPYTRKHALEWIDLVGKPGTRENPQVHFVIDVEGEAAGGIGLLMQTDVSRVGGELGYW
                     LGKRFWGRGIMTRAIAGMTDWYFQNLPITRIYALVFDYNQPSARVLEKCGYTLEGRLK
                     KAAIKEGKVIDELLYARVIPD"
     gene            2203..2760
                     /locus_tag="PKW73_07930"
     CDS             2203..2760
                     /locus_tag="PKW73_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73251.1"
                     /translation="MAEKGDRLQVAGEHVNTAGKGDRLDHKTTADDGALQCLQGDCNL
                     AQAGNSTLAKPKEACAKGEKPVVDIKAAYGSDEGFLRKSPQEMRFNAGLKPNASSEEL
                     FNAMGKQVADFYTNAKPGDKALMQQNYGLADKDFTPAKVAASIVDSHRKEFNMPGASA
                     SEIEKALHKSILHKINTCQMEIDPD"
BASE COUNT          792 a          810 c          766 g          571 t
ORIGIN      
        1 agcctcaccc tggaaaatct agccgagatt tactggcggc gccgcaagta cgacaaagcc
       61 gaacccctct gcaagcaaat cctgcaaatc gccgaaagcc tgcttggtcc agagcacagc
      121 gatgttggtc tggcagccaa taatctagct attctctgcg aaagacaggg caagcactcc
      181 gatgccgcca ttctctatca gcaggccctg actattgccg agaacattct cggcaacagt
      241 catccggaca cggaaattct cagagagagc catgcccggg cccgcttcca ggctctcaaa
      301 caggtggaac aaaagctgga agaatcccac aaaggcagcc gctggagcaa atcaggctgg
      361 tggaaagcct accaacaata gattcctgct tgataaatgc ctcaatataa gcctccgtcg
      421 atttcaacga aacgcaggcg cagcttgagg cggcgcacat ggagcaaaca ctgcacggtg
      481 aggagcttat ggcaccgacc ggacagagcc gcttccaact ggctgccagt caagaaagaa
      541 gccttgcgca agtccttcct gggaatgaaa taaccaagat agaaacgcga gacttcaccc
      601 agcttgccgg ccagatctcc tgtgccaaat gcgtagacgg gaggctccac agcaatgggc
      661 agttccttga gaggcaccac gtccagtgca attttcaact tactcctcct ggctatcttc
      721 tccagggtgg ccaccgtagg catcttccaa tcgagtatga aagctgccag agaggcataa
      781 tctacctcgg tctgataagc cacaagatca atgagctcga gaagatcgtc ggtcttatag
      841 atgcgtaaaa gatcctgcac cagtgttctg gtggcgcgag ccagatgcag ctcgcacaac
      901 cgtttaacgt attgtttgcg gctgccttgc aagagaccgg tccattgacc tttcactacc
      961 tgatagctca gctcggcgat atagcttctt tgagccgagc cgacatggcg ctccggcttg
     1021 gaaatagatc ttgcgattat gactacattg ttttttccag acataaaaca tacctcgtga
     1081 aactgggtgc tcatgtcgcg gcaaagaaag cggccggtac tgccgactga acggcggcac
     1141 cggcaaaaga accgtgcctc caggcaactg ctcaagaacc tcctgagacg gcagcaaaag
     1201 acccgccagc cttggatgct cgcgggggaa ttcaaaagtg ctgctcaatc aattctcttg
     1261 ggggctgtcc ctgaaatttc gctccggttc cggcctgtct ttctgcgact gataacgcca
     1321 acctagaaaa tcgccgctgc cccagtacag tcatttcggc aggccggggc aaaatttccc
     1381 tggggctgtc accatcagca ccttcgttag acggctgtga gagccgcccc agcctttctc
     1441 agctagcgcc cttgaagaaa gtcgcgcaag ccctataatc tttgccatga cacgtaaaaa
     1501 cacgattatc gattgcggcg atttcactat tcgcagcctg gtcaaagctg atgccgattc
     1561 gctcagtgcc aatgccaacg actacgacat ctggctcaat ctgagagacc gctttcccca
     1621 cccatacact cgtaagcatg ccctggagtg gatcgacctg gtgggcaagc cgggcaccag
     1681 ggaaaacccc caggtacact tcgtcataga tgtggaaggt gaggcagccg ggggcatcgg
     1741 cttgctcatg cagaccgatg tcagccgggt aggaggcgaa ctgggttact ggctgggcaa
     1801 acgcttctgg gggcgcggca taatgaccag agccatagcc ggcatgaccg actggtactt
     1861 tcaaaatctg ccgattaccc gcatctatgc cctggtgttc gattataacc agccttccgc
     1921 ccgagttctt gagaagtgcg gttataccct ggagggaaga ttgaagaagg cagccattaa
     1981 agagggcaaa gtaatcgatg agttgctcta tgccagggtc ataccggact gacgtaaggc
     2041 tctaacctga aaagggtcgc tatagcccga gaacaggcgc gcaagctatc ccttgaggat
     2101 tagggatggg gagcgggggg tatgaagaga gggcgggtaa ctaccagtat ggcgactatg
     2161 ccggaatgac aaaccaccgg caaaaacaca ggggacaata tcatggccga gaaaggtgac
     2221 agacttcagg tagcaggcga gcatgtgaac accgccggta aaggcgacag gctcgaccac
     2281 aagacaacag ctgatgacgg cgccttacaa tgcctgcagg gcgactgcaa cctggcccag
     2341 gccggcaatt ccaccctggc aaaaccaaaa gaagcctgcg ccaaaggtga aaaacctgtg
     2401 gtagacatca aggcggccta cggctccgat gaaggcttcc tcagaaaaag cccacaagaa
     2461 atgcgattca atgcgggatt gaagccaaat gccagctctg aagagttatt caatgccatg
     2521 ggtaagcaag ttgctgattt ctacaccaac gccaaaccgg gcgataaagc tctcatgcaa
     2581 cagaattacg ggctggcgga caaagacttt acacctgcca aagtagcggc cagcatagta
     2641 gactcgcacc ggaaagaatt caatatgcct ggcgccagtg cctcagaaat tgaaaaagct
     2701 ctgcataaga gcatcttgca taaaatcaac acctgccaga tggaaattga cccggactga
     2761 cagtgaccaa gctcaaagtt gccgccatag tatcaacggt tgccctggca ctctctttag
     2821 gaattgccct ctactggccc cagcttcaaa aaggcttgcc gcaaaaggag caggctgatc
     2881 ccactctggt agaaatgcca ccgccagcta gagaaagaga agaccaccag ctcttttat
//
LOCUS       DAOQNZ010000384         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18036_length_2933_cov_3.351981, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000384 DAOQNZ010000000
VERSION     DAOQNZ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18036_length_2933_cov_3.351981"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /gene="glgP"
                     /locus_tag="PKW73_07935"
     CDS             complement(<1..1678)
                     /gene="glgP"
                     /locus_tag="PKW73_07935"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HNA73252.1"
                     /translation="MEERPKRKRGADTVEMNALNTNEAVRKHLLENLRSDQGKFAQVA
                     STNDYYMALARVVRDRLMRRWTSTARTYFEKSSRTVVYLSAEYLIGPQLGNNLINLGI
                     YDDTETTLLGLGLSLDQLMEREPEPGLGNGGLGRLAACYMDSLATLQIPAIGYGIRYE
                     FGIFSQAIRDGWQTEMTDKWLKYGNPWEIANADVAFDVYLGGHTVACEGGSGVRWVPE
                     RVVTGVAYDTPVLGYGVNTANLLRLWKAEASESFDFQAFNVGDYYRAVDAKVYSENIT
                     KVLYPNDEPLAGKQLRLEQQYFFVTCSLQDMIRIYLQREKNFDRFHEKYAVQMNDTHP
                     ALAVAELMRLLVDVHGVPWEKAWFITTNTMSYTNHTLLPEALETYSLPIFQRLLPRHL
                     EIIYEINARFLGEVGLRHPGDPALLSRLSLIGETGEKQVRMANLACVGSHAINGVAKL
                     HSDLLKSTVLKDFYLLFPDKFTNVTNGVTPRRFMLLSNPELSDLISEAIGDSWPRHLD
                     QLRQLEPLAEDSSFCTKWATVKLKRKQILAARIKEVTGLEANCWSLFDVQT"
     gene            1941..>2933
                     /locus_tag="PKW73_07940"
     CDS             1941..>2933
                     /locus_tag="PKW73_07940"
                     /inference="COORDINATES: protein motif:HMM:NF020921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73253.1"
                     /translation="MTVASEDRKTTELAVDQICAYGIARSTVKVDAANPPLGQEELRN
                     TDAYWRACNYLAAGMIYLRDNPLLKQPLKVEHIKNRLLGHWGSSPGLSFVYVHLNRLI
                     KKYDLDMIYMAGPGHGAPGVLAPVYLEGTYSEIYPDKSEDEEGMAAFFKQFSFPGGIG
                     SHCTPETPGSIHEGGELGYSVSHAYGSVFDNPDLITAVVVGDGEAETGPLATSWHSNK
                     FINPARDGAVLPILHLNGYKINNPTILARVDHEELEALFHGYGYTPYFVEGSDTDSMH
                     QAMAATLEKCITEIRAIQKEARENAANNLPVKRPRWPMIVLRSPKGWTGPKELSG"
BASE COUNT          735 a          814 c          740 g          644 t
ORIGIN      
        1 tggtctgcac atcaaaaaga gaccagcaat tagcttccag tccggtcact tctttaatgc
       61 gagcagccag aatttgcttg cgtttcaact tcaccgtcgc ccacttggtg cagaaactgc
      121 tgtcttcagc caggggctcc aactgcctca attgatcaag gtgacggggc caactatcac
      181 caatggcctc gctgattaga tcgctcaatt cgggattgga gagcagcata aaacggcgcg
      241 gcgtcacgcc gttggtgaca ttggtgaact tatcaggaaa gagcaaataa aagtctttca
      301 gcacagtgct cttaagcaaa tcgctgtgca acttggccac accgttgatg gcatgactgc
      361 ccacacaggc caggttggcc atgcgcacct gcttctctcc cgtctcgccg atgagagaga
      421 gccgcgagag cagtgccgga tcaccggggt gacgcaagcc cacttcgcca aggaacctgg
      481 cattgatttc atagataatt tccagatgtc tgggcagaag cctctgaaaa ataggtagac
      541 tgtatgtttc cagggcttca ggcaaaagcg tatggttggt gtaagacatg gtattggtgg
      601 tgatgaacca ggctttctcc cagggcaccc catgcacatc gacaagaaga cgcatcagct
      661 ccgccaccgc taaagccgga tgggtatcat tcatctgcac cgcatacttt tcatggaagc
      721 ggtcgaaatt cttctcgcgc tgcaagtaaa tgcgaatcat gtcctgcaga gaacaggtga
      781 caaagaaata ctgttgctcc agacgcagtt gtttgccggc cagcggctcg tcattgggat
      841 agagaacctt ggtgatattt tcggaataaa ctttagcgtc cacggcgcgg tagtagtcac
      901 cgacgttgaa tgcctggaag tcgaaggatt cggaggcttc cgccttccat aatctcagta
      961 gatttgcggt gttgacgccg taacccaaga ccggcgtgtc ataagcaaca ccggtaacta
     1021 ctcgctccgg cacccacctg acaccactgc cgccttcgca agcaacagtg tgaccgccca
     1081 gatagacatc gaaagccaca tcggcgttgg cgatttccca gggattgcca tacttgagcc
     1141 acttatccgt catctcggtt tgccagccgt ctcgaatggc ctgactgaaa ataccgaatt
     1201 cgtatctgat accgtatccg atggcaggta tctgcagagt ggccagagaa tccatatagc
     1261 aggcagccag ccgtcccaaa ccgccattac ccagacccgg ctcgggctct cgttccataa
     1321 gctggtcgag actcaaacca agacccagca gggtcgtctc ggtatcgtca taaataccca
     1381 ggttgataag attgttgcca agctgcggac cgattaaata ctcggctgaa agataaacaa
     1441 cggtgcggct gctcttttca aaataggtac gagcagtgct ggtccagcgc cgcatcaagc
     1501 gatcgcgcac taccctggcc agggccatgt aatagtcatt agtggatgcc acctgagcaa
     1561 acttgccctg gtctgaacgg agattttcaa gcaggtgctt gcgaaccgcc tcgttggtgt
     1621 tgagcgcatt catttcaacg gtatcggcac cgcgcttgcg cttagggcgt tcttccacaa
     1681 ctcctcctcc gggccggggc ccgagatcca ggtctcattg ctagttcttt acaaatagct
     1741 actttaaaag ccttcctgca accagctatt ttagaccggg cggacaattc taccctcatt
     1801 cccaagcact atatacaaac tcatcctccg gaacgagatt aatatcggtc cggggcggta
     1861 agcttacaca ttaaaaccag tgcgccaggc aattcatttc gcccggccgt ggatagatgc
     1921 cctcaccgga gtaataagag atgacagttg cgtctgaaga tcgaaaaact acggaactcg
     1981 ctgtagatca aatttgtgcc tatggtattg ctcgctcaac agtgaaagtg gatgcagcca
     2041 acccgccatt agggcaggag gaacttcgca acaccgatgc gtactggcgc gcctgcaatt
     2101 atctggcagc cggcatgatt tatctgaggg acaacccact gctcaaacag cctttgaaag
     2161 tcgaacatat taagaaccga ctgcttggac actggggttc cagcccgggt ctcagctttg
     2221 tctatgtgca cctgaaccgt ctcatcaaaa agtacgatct ggacatgatt tatatggccg
     2281 gaccaggaca tggtgcgccg ggagttctgg cacctgtcta cctcgagggc acctattccg
     2341 aaatttatcc cgataaaagt gaagacgaag aaggtatggc cgctttcttc aaacaattct
     2401 cttttccggg cggtatcggc agccattgca cgccggaaac accaggctcc atccacgaag
     2461 gcggcgaact gggctacagc gtgtcccatg cttatggctc ggtcttcgac aatcccgact
     2521 tgatcacggc tgtcgtagtg ggtgacggtg aagcggaaac cggtcccctg gcaacctcct
     2581 ggcattccaa caagttcatc aacccggcta gagacggagc tgtgctgccg attttgcact
     2641 tgaacggcta caaaataaac aaccccacca tcctggccag ggtagatcac gaggagctgg
     2701 aagccctctt tcacggctac ggctacacac cctatttcgt ggaaggtagc gacaccgaca
     2761 gcatgcacca ggccatggcc gccactttgg aaaaatgcat caccgaaatt cgcgccattc
     2821 aaaaagaagc ccgggaaaac gctgccaaca atcttcctgt taaaagaccg cgctggccga
     2881 tgatcgtcct gcgcagcccg aaaggctgga ccggaccgaa agaactctcg ggt
//
LOCUS       DAOQNZ010000385         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18040_length_2932_cov_3.773375, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000385 DAOQNZ010000000
VERSION     DAOQNZ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18040_length_2932_cov_3.773375"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PKW73_07945"
     CDS             <1..1047
                     /locus_tag="PKW73_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNA73254.1"
                     /translation="LGLALAAVASLTAHFAMGYFSQSKEVLDSGRAYLAAYSFVLLPF
                     SVTVIANAAFRAIGDSKTPLYIVSAMTAVNIAGDYLTVIYNWPVPGLGIRGMAYAALL
                     GSGLGAVLALYFLHRSQLKPALKALFPPDKKMLRRICNIGIPSAMQRLFWALSVFVIF
                     FILMGCPHSVQALASWTIGMRVESLVFMPLMALSLSVSSIVGQNLGAGETARAYKAGW
                     RVTGIGILMMVAAALTLYFGAEQIAGSMTREPEALKYTSDYLRINALAEPFLALAMIL
                     AGALQGAGETRTPMLLTIFTNWLIRIPLAYVLALPLKLGVTGVWWAMTASIVIHGILM
                     TLRYQAKDWIKEKI"
     gene            1051..>2932
                     /locus_tag="PKW73_07950"
     CDS             1051..>2932
                     /locus_tag="PKW73_07950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNA73255.1"
                     /translation="MTEANLEQGASKTGKRKGDKFLFCLLSILACIGLFGYLKFQDSV
                     FPSASIKFAVSKAEIMQISDRWKADLKIPGDYPITSCVFNLDEGAKNFLDFKLGTREA
                     NRLMKDQAPVFYWDISYAKPMNQETVQAQISPDGKLVAFVFYIDKDRKLPSLSRDEAL
                     SMARRFVEAQSGFKEALWDIEKEETRPQSGRVDHRFVFQHKEIDYAGAKLRIAVSFSG
                     NLMTEYNSMVHLPDSWSQEYGKMRSRNELLQSIATIFYVILHPLAFYIAFSQWQKGNV
                     RIRFALFAAAVLTVIFLANNYNDFPLRLASYSSEKSYQSFLIQTLSTPLLNAIMVFCL
                     SAVLAAAGEIIYRKTFPEKIALEDSLSLKGLASEEGLKGLYLGIIWCAVSLGYQIAYY
                     WIGRKVGYWCPIQLDNYQVLGSYLPWIGAVSLGVFASTSEEILYRVLFLGLCKPIVKN
                     FWLANFLQAAAWGFMHSSYEQQPCYARGLELTIEGMLDGFILRRFGLLPCIVSHYLFD
                     AFCCVTPLFKAPPSLMVSAAVPFAPVLVLLFLGLWQRGKLAAPLKNSDLPVKEPQHTA
                     EEEEEAHIHYEPLKKGRRWQLALVIALGLSLAVFADRKIAVLGENLKPLKIERHKAIE
                     TAR"
BASE COUNT          667 a          836 c          738 g          691 t
ORIGIN      
        1 ctgggtctgg ctctggcggc agtggccagt ttgacggcgc atttcgccat gggctatttc
       61 agccagtcaa aagaagtgct cgactccggc cgggcttacc tggcggcata cagctttgtg
      121 cttttaccct tcagcgttac cgtcatagcc aatgccgcct ttcgtgccat tggcgacagc
      181 aaaacaccac tttatatagt ctcggccatg acggcggtca atatagcagg cgactacctg
      241 accgtcatct ataactggcc ggtgcccgga ctgggtatcc ggggcatggc ctacgcggcc
      301 cttctaggca gcggactggg cgcggttctc gctctttact ttttgcaccg ctcccagttg
      361 aaaccggctc tcaaagcgct cttcccccca gacaagaaaa tgctgaggcg gatctgcaat
      421 atcggcatcc cctcggccat gcaaagactc ttctgggcgc tgtccgtatt tgtcatcttc
      481 tttattctca tgggctgtcc ccactcggta caggccctgg cctcctggac tatcggcatg
      541 cgcgtggaat cgcttgtctt tatgccgctc atggcccttt ccctctctgt atcttccata
      601 gtcggtcaaa atctaggagc cggagaaaca gccagagcct acaaggccgg ttggcgagtc
      661 accggcatcg gcattttgat gatggtggca gcagccctga ccttatattt cggagccgag
      721 caaatagccg gcagcatgac cagagaaccg gaagccctca aatatacttc cgattatttg
      781 cgcatcaatg ccctggccga acccttcctg gccctggcca tgatcctggc aggagccctg
      841 cagggtgcgg gggaaactcg tactccaatg ctcctgacca ttttcaccaa ctggttgata
      901 cgcatccccc tggcctatgt actggccctt cccctcaaac taggtgtcac cggagtctgg
      961 tgggcaatga ctgcctctat agtcattcac ggaattttga tgaccctcag gtaccaagca
     1021 aaagactgga tcaaggaaaa gatataggcc atgacagagg caaatttaga gcaaggcgca
     1081 agcaaaaccg gcaagcggaa aggcgacaag ttcctcttct gcctgctctc gatactggct
     1141 tgcatcggac tattcggtta tctgaaattc caggactcag tttttccttc cgcctccata
     1201 aaatttgccg tcagcaaagc ggaaatcatg caaatttccg accgctggaa agcagatcta
     1261 aagatccccg gcgactatcc catcacctcc tgtgttttca atctcgacga aggcgccaag
     1321 aacttccttg atttcaaact gggcacccgg gaagccaatc gcctcatgaa agaccaggcc
     1381 cccgtcttct actgggatat cagctacgcc aagcccatga atcaggaaac ggtgcaggcg
     1441 caaatttcgc cggacggaaa actggtggcc tttgtcttct atatagataa agatcgtaag
     1501 ttgcccagcc tcagccggga cgaagcactc tccatggcca ggcgttttgt ggaagcacag
     1561 agcggcttca aagaggcgct ctgggatata gagaaggaag aaacccgccc gcaaagcggt
     1621 cgcgtcgatc accgctttgt cttccagcat aaagaaattg attatgccgg cgccaagctg
     1681 agaatagcag tcagcttcag cggcaacctc atgaccgaat acaacagcat ggtgcatctg
     1741 cccgacagtt ggagtcaaga gtacggcaag atgcgctccc gcaatgaatt actgcagtcg
     1801 atcgccacaa ttttctacgt aatcctgcat ccgctggcct tctatattgc cttcagtcag
     1861 tggcagaaag gcaatgttcg catccgcttt gccttgtttg cagcggcagt cttgaccgtc
     1921 atcttcctgg ccaataatta caacgacttc ccgctgcgat tggcctccta cagcagcgaa
     1981 aaatcttacc agtcctttct tattcagacc ctttcaacac cgctcttgaa tgccattatg
     2041 gtcttctgcc tttcggcggt actggcagca gccggcgaaa ttatctaccg aaaaaccttc
     2101 cccgaaaaaa ttgccctcga ggacagtctc agcctcaaag gtctggccag tgaggagggt
     2161 ctcaagggac tctacctggg cattatctgg tgcgccgttt ccctcggcta tcaaattgcc
     2221 tattactgga tcggcagaaa agtcggctac tggtgcccga ttcaactgga taattaccag
     2281 gtgctgggtt cctacctgcc ctggattgga gcagtaagcc ttggtgtctt tgcctcgaca
     2341 tcggaggaaa tactctatcg ggtgctcttc ctcggtctgt gtaagccgat cgtgaagaat
     2401 ttctggctgg cgaacttctt acaggctgcc gcctggggct ttatgcatag tagttatgaa
     2461 cagcagcctt gctatgccag aggactggag ttgaccatcg aaggcatgct cgacggcttc
     2521 atcttaagac gtttcggact tttgccctgt atagtctccc actacctctt cgatgccttt
     2581 tgttgcgtca cgccgctatt caaagcccca ccctccttga tggtatcggc ggcagtgcct
     2641 tttgcaccgg tacttgtact gcttttcctg gggctctggc agcgagggaa actggctgcc
     2701 cccctcaaga atagcgatct accggtgaaa gagccgcagc atacggcgga agaagaagag
     2761 gaagcacata tccactatga gccgctcaaa aaagggaggc gctggcaact ggccctggtc
     2821 attgccctgg gtctgagcct ggccgtcttc gccgaccgga aaattgccgt gctgggagag
     2881 aatctgaaac cgctcaagat tgagcgccac aaagccatag aaacggcaag ag
//
LOCUS       DAOQNZ010000386         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18042_length_2932_cov_3.496698, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000386 DAOQNZ010000000
VERSION     DAOQNZ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18042_length_2932_cov_3.496698"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKW73_07955"
     CDS             <1..489
                     /locus_tag="PKW73_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73256.1"
                     /translation="LVAVGHTANYIAAQRKKGMQFYLAIFTCEEQSACYPAPAHWANV
                     LLECQKDYPQLARDMDSALADLKKLTFAQLEKECGFSLADVHSLGKEDHRYIDLERYL
                     ASDRSTAMLRRFLYHCCRLTELYSGDGWTYTVDGQACLTEFVMPNLELTQLSDLHLYH
                     LQ"
     gene            712..1938
                     /locus_tag="PKW73_07960"
     CDS             712..1938
                     /locus_tag="PKW73_07960"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNA73257.1"
                     /translation="MKENEFEAPQQRLDPTADGKLQETAYAFGREVKMISLGVGEAVK
                     DTLEHPQSKLNELATSAAVGTGLGVMSRLGAPGRIIALGAGTAMTTKFAYDELTGERW
                     SKFGAAVKDTWQSNENMDKNIALTRDSLGSFLVDTGVGLAAKGLSSRVTARLAPPSLL
                     VDYAIRKADSDGGKALLALQNRWEKTYNLSNYRDGKLQISGFTQSAVPGQARGDLLRV
                     GKTPDGKVILSAMDVEGHGIGAAKKAVTVHAAMDAVLPKAKGKDASEILALVDKKLDA
                     SDERAITAALSTFDPVTNKLDTATASSQFAFLIRKNGRVHQLDAKGGGLGLGNDMYSL
                     MKPGHEVVRLNHGDTVVMASDGVYDRFGYGNLRAFKNFLRSVGPNPDKLRRLILNKPV
                     AETGADDTSFLIFNRP"
     gene            complement(2271..2798)
                     /locus_tag="PKW73_07965"
     CDS             complement(2271..2798)
                     /locus_tag="PKW73_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73258.1"
                     /translation="MNTLNFLDPLLSVASHGETRDSHRRSFWTGIKSEYLLPDFFSLA
                     GKAVPGANTGLEQALSAFFKPEVVRFLLQLPNTYWFYRLLRESVMVRAASPVSVDVAD
                     DRIDGAMMNLAVQRLRAKHVDGFDLSMPLLSLSPAELCARSVRGLILLRPRSDGKTEQ
                     IEVNPLLAAAAPVVA"
BASE COUNT          750 a          818 c          800 g          564 t
ORIGIN      
        1 ctggtagcgg tcggtcatac cgccaactac attgccgccc agcgcaagaa agggatgcag
       61 ttttacctgg ccatctttac ttgcgaagag cagtcagcct gttatccggc tcccgctcac
      121 tgggccaacg tactgctcga gtgccaaaaa gattacccgc agctggcccg ggatatggac
      181 tccgccttag ccgacctcaa aaagctcacc ttcgcccaac tggagaagga gtgcggcttc
      241 agcctggcag atgtccatag cctcggcaag gaagaccatc gctatatcga cctggagcgc
      301 tacctggctt cagacagatc gactgcgatg ttgcgccgtt ttctctatca ctgctgccgt
      361 cttaccgaac tttacagcgg agacggctgg acatacacag ttgacggcca ggcatgcctg
      421 acggaattcg tcatgcccaa tttggaactg acgcaattga gcgacctgca cttgtatcat
      481 ctccagtaac ttttaaacca aagaggaaag cgacctgcta acaacaggcc gcttttcgtc
      541 tttaaaaaat ttcgccattt tactctattg cgtagtatta cgcaaacagc ggagccctgc
      601 atttttaacc atttggacaa atagtcacac cttcaacaca gttgggtcgt atcatgcttt
      661 cagtctttca atttaaggag tcactggaac gcccccgcga ggtcatcctg tatgaaagag
      721 aatgaattcg aggcgccgca gcagaggctc gaccccactg ccgacggcaa gttgcaagag
      781 accgcttacg ctttcggtcg cgaagttaaa atgatttctc taggggtggg tgaggccgta
      841 aaagataccc tcgaacatcc ccagagcaaa ctgaacgaac tagccacatc agccgctgtc
      901 ggcaccggtc ttggagtaat gagccggttg ggagcgccgg gtcgcatcat cgcactgggc
      961 gcgggtaccg ccatgaccac taagtttgcc tacgatgaat tgaccggtga gcgttggagc
     1021 aagtttggag cagcagtaaa agacacctgg caatctaacg agaacatgga caaaaacatt
     1081 gccttgacca gagacagcct gggctccttc ctggtggaca ccggcgtcgg gctggcggct
     1141 aaaggtctga gcagcagagt cacagcccgt ctggcgccgc cttccctgct ggtggattat
     1201 gccatccgca aagccgattc cgatggcggc aaagcgcttc tcgccctgca aaaccgctgg
     1261 gagaagacct acaatttgtc caactatcga gacgggaaat tgcaaatctc cggcttcacg
     1321 cagtcggccg tacccggcca ggccagaggc gacttgctaa gagtggggaa aactccggac
     1381 ggcaaggtga ttttgagcgc catggacgtg gaaggtcacg gcatcggcgc agccaagaaa
     1441 gcagtcaccg tgcacgccgc catggacgcc gtgctgccga aagcaaaagg caaagatgcc
     1501 agtgagatac tagccctcgt cgacaaaaag ctggacgcaa gcgacgaacg cgcaattaca
     1561 gcggcgctca gtacctttga tccggtgact aataaactgg acaccgccac tgccagttcg
     1621 cagtttgcct ttctaattcg caaaaacggt cgggtgcacc aacttgatgc caaaggcggc
     1681 ggtctgggtc tgggtaatga tatgtactcg ctcatgaagc ccggccatga agtggtgcgt
     1741 ctcaaccacg gtgatacggt agttatggcc agtgatggag tctatgaccg atttggttac
     1801 ggcaacctgc gcgccttcaa aaacttcctg cgctcagtcg gtcccaatcc agacaaactg
     1861 cgccgcctca ttctcaacaa gcctgtggcg gaaaccggtg cagacgacac cagtttcctt
     1921 attttcaacc gaccttaaaa cctttcctcc cacagcagca gaaacaaaca gcagcctcta
     1981 cgacctgcta tcgtaaccca aaccagtcac gcaaaaaaaa tgctttccct ggcgaggcaa
     2041 ggctttcagt gcatactaca ttttttagta tctggctgca aaagagaaat agaaagagaa
     2101 gtgaaggaag gaaggaagga aagcaagcaa acaagagaga gagaagagga tgtccatgaa
     2161 tgaacaaagg cagggaagaa gcgtgggctt cctccctgcc tggccggcgt gtccgtcaac
     2221 tagccgaaag actgccggaa gaagtcatca gaaccttgcc cgcggcgact tcaagccacc
     2281 accggtgccg ccgcagccag aagcggattc acttcgatct gctcggtctt gccgtcgctc
     2341 ctggggcgca gcaggatcag accgcgcacc gagcgtgcgc aaagctcagc gggactgagg
     2401 gaaaggagcg gcatgctcaa atcgaaacca tccacatgct tggcacgcag gcgctgcacc
     2461 gccaggttca tcatggcccc atcgatgcgg tcatcggcga catccaccga caccggcgaa
     2521 gcggcgcgca ccatgacgga ctcacgcaga agtcggtaga accagtaggt attgggcagt
     2581 tgcagcagaa agcgaaccac ctcgggcttg aagaaggcag aaagcgcttg ctccaggccg
     2641 gtgttcgctc cgggcacggc cttgccggcc agggagaaga agtcaggcaa gaggtactcg
     2701 ctcttgatgc ccgtccagaa ggagcgccga tgggaatccc gggtctcgcc atgggaagcc
     2761 acggacagga gcggatcgag aaagttgagg gtgttcaaat cgcttctcca gtgaaattac
     2821 tttgccatgc cgggggctta cacgctgtca tcccgacaga aactaaagag cctgaaacaa
     2881 gtaggttagt ggtgctggtt gcttcctctc aaaaataaaa gccgaaaccg gc
//
LOCUS       DAOQNZ010000387         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18074_length_2929_cov_3.216423, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000387 DAOQNZ010000000
VERSION     DAOQNZ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18074_length_2929_cov_3.216423"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKW73_07970"
     CDS             complement(<1..317)
                     /locus_tag="PKW73_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73259.1"
                     /translation="MAVTQVPIVLPAEKEWYELEPEYFTRRISTVCGMLPGYTWAKGL
                     KIDRVVSGSRNFRQPTNMGMSLFDGCSVLVISEDSPISGRQIMQEASINCDFKERESG
                     LEIL"
     gene            complement(598..1098)
                     /locus_tag="PKW73_07975"
     CDS             complement(598..1098)
                     /locus_tag="PKW73_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73260.1"
                     /translation="MIEEEGSRRLGKTMAGLPACLLSAVVWNFLGTGFLYASCGVCFQ
                     VIRLEVPYEKELFRFFIPFLIGLLLSRVHFDYVRLAVKHGAELNNFARASRLKTMCVA
                     GALTWYGTACNCALLLFWMMHFDYELVDFAIVALIATVGLAIGTQYYCDALGNPIVSL
                     KTPGEH"
     gene            complement(1166..1918)
                     /locus_tag="PKW73_07980"
     CDS             complement(1166..1918)
                     /locus_tag="PKW73_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73261.1"
                     /translation="MQNQASELIETLPEGSLRFGLRVSPLLIRALTSIAAIATNVLNL
                     AVCFYVYVFILFATSFSQPFSYLTAPLAACLIGILSTRINKSYIESSHKLIGRDASPD
                     HDLIQMRFLTVVGFFCSTMFTLSILTPALSWPFATVLLLSSWIGILGVRNCLNKTLSL
                     AGNSSSALPAVPKFADDLEVMLVSLCKADSSQMANALARECLNSLKRKVSGDTPGDEV
                     HTLVNELMKRRRHEDADMISAHYLKLLEAEGE"
     gene            complement(2047..2466)
                     /locus_tag="PKW73_07985"
     CDS             complement(2047..2466)
                     /locus_tag="PKW73_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73262.1"
                     /translation="MTQSLDISSLPLARISELAEFLDVSAANAEAIFRKLERAGDGFL
                     PEDDAATDDERSGRTEGVGRVSRLAQTLAAEVEKVYRREMDKLTACASTLERWRKSSP
                     SATEAGEIERLGWELTQLQSKYLRVIEFASRLSSAKL"
     gene            complement(2542..2793)
                     /locus_tag="PKW73_07990"
     CDS             complement(2542..2793)
                     /locus_tag="PKW73_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73263.1"
                     /translation="MKSFTDQKVLIARQKAKRLRMVFLVFALVTMCDILTIALLQYAG
                     IITERLKLLVFLVLVGLASPLGVVLAIVCARAESRAKNR"
BASE COUNT          828 a          784 c          665 g          652 t
ORIGIN      
        1 agaatctcta agccggattc tctttccttg aaatcacaat tgatagaagc ctcctgcatg
       61 atctgccggc cacttattgg tgaatcctcg gaaatcacca gcacggagca accatcaaat
      121 aaagacattc ccatgtttgt aggttgtcta aagttgcgag aaccagatac cacccgatct
      181 atctttagcc cctttgccca ggtatatcca ggcaacattc cacaaacggt gcttatccgc
      241 ctggtaaagt actctggctc aagctcatac cattcctttt cagcaggcaa aacaatgggc
      301 acttgagtaa cagccacagt ttccgtgtcg gccggtagcc atcgcaaaaa ctcaagtatt
      361 tgcggtgaaa gcgaaactgt ggatacgggt tccaggtctc gtgctctcgc tgctgccgga
      421 ttccagcaac tactgactag gaaaactgcc agagcaaagc tcagccatgt cggtacaatt
      481 actctcaatc tccataagcc cttaaactgg tgactggaat aagcatagac aaccagagat
      541 gaatagcgcc gcagaagaaa tcactttcgc aactgatctc tcctgtggca agcagactca
      601 atgctcgcct ggcgtcttca aactaacgat cgggttgccc agagcatcgc aatagtattg
      661 cgtaccaatt gccaggccta ccgtggcaat caatgcgact atggcaaagt ccacgagttc
      721 atagtcaaaa tgcatcatcc agaacaataa cagagcgcaa ttgcaagccg tcccatacca
      781 ggtaagagcc ccggccacac acatcgtttt aagccggctg gctctggcga agttattgag
      841 ttcagctcca tgcttaaccg cgagcctgac atagtcaaaa tgaacccgag aaagcagcaa
      901 ccctatcaag aacggtataa agaagcggaa tagctccttt tcgtacggaa cctccaaacg
      961 aattacttga aagcatactc cacaagatgc atacaagaac cccgtaccta agaaattcca
     1021 gacgacggca ctaagcagac aagccggtag tccggccata gtttttccca agcgccggga
     1081 gccttcttct tcaatcactg gattaacccg ccgcactcag attcgactaa caactctcaa
     1141 aagctcaggt caccaaaaga ctacgtcact ctccttccgc ttccaaaagt ttgaggtaat
     1201 gagcggaaat catatcggca tcctcatggc gcctacgttt cattagctca ttgacgagag
     1261 tatgcacttc atcaccaggg gtatcgccgc tcacttttcg cttcaaacta ttgaggcatt
     1321 cccgggccag cgcgttagcc atctggcttg aatcggcctt acatagactg accagcatca
     1381 cttccagatc atctgcaaat ttcggtactg ccggcagcgc ggagctagaa tttccagcaa
     1441 gggagagggt cttgttcaag caatttctta cacctaaaat gcctatccaa ctgctaagca
     1501 gcaagaccgt ggcaaagggc caggataaag ccggtgtgag aatactgagg gtgaacatag
     1561 tgctgcaaaa gaaacccacc accgtcaaga accgcatctg aatcagatcg tgatcggggc
     1621 ttgcatcacg gccaataagc ttgtgcgaag attcaatgta gctcttgttg atgcgagtag
     1681 acagaatacc aatcaaacaa gccgcaaggg gagcagtgag gtatgagaaa ggctgactaa
     1741 agctggtcgc aaacaggata aaaacataaa catagaagca caccgccagg ttcaatacat
     1801 tggtggcgat ggccgcaatc gaagtgagag cacgaatcag gagtggcgac actctcagac
     1861 cgaacctcaa tgaaccttca ggaagagtct cgattaattc ggaagcttgg ttttgcattt
     1921 acttgttcac cctggtcgaa gacaaggaca ctgatctcag tttagtcgcg agcaactgag
     1981 ttcgccagat aaaactgcat ctggtgtaaa cattgtcaag ccagggatca aaatgtaagg
     2041 tgcaagttat agtttggctg aagaaagtct ggaagcaaac tcaatcactc tcagatactt
     2101 gctctgcaac tgagtcagtt cccaacccag tctctcaatt tcaccagcct cagtagcact
     2161 ggggctactc ttacgccaac gctcaagcgt agaagcgcag gctgtcagct tatccatttc
     2221 acggcggtac accttctcaa cttcagcagc cagagtctgc gccaggcggc tgacccgtcc
     2281 tacgccctcg gttctaccgc tgcgttcatc atcggttgcg gcgtcatctt caggcagaaa
     2341 gccgtcaccg gctcgctcca acttgcggaa gatggcttcg gcattggccg cggatacatc
     2401 gagaaattca gccagttcac tgattctggc aagcggtaaa ctcgaaatgt cgagactttg
     2461 ggtcatgagg ctttccctaa tttattacat ttggaaacta gagactttat gcccggcggg
     2521 agcaaatatc gccgccctaa ttcacctgtt tttggcccta ctttccgcac gggcgcaaac
     2581 aatggccaga accacaccca gagggctggc aagaccgaca agaaccaaaa atacaaggag
     2641 tttcagacgc tctgtgatga tgccggcata ctgcaaaagc gcaatggtca aaatgtcgca
     2701 catggtgacc agagcaaaga ccaggaagac catacggagg cgtttggctt tctgccgggc
     2761 aatcaaaact ttctggtcag tgaaggattt cataaatgct tcccgcgaca gaacctaact
     2821 ttcatatgcc acgggaagcc tgccctgctt cattatgggg acaacaccag cccttactgg
     2881 ggctttcccc agaaatttta tcccctgaaa ctgcaaaatt gcggcccta
//
LOCUS       DAOQNZ010000388         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18119_length_2925_cov_4.185714, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000388 DAOQNZ010000000
VERSION     DAOQNZ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18119_length_2925_cov_4.185714"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PKW73_07995"
     CDS             complement(<1..1060)
                     /locus_tag="PKW73_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNA73264.1"
                     /translation="MMQIDRLVFPEHFKTNVPVARQIAEVMKEGILQGLVSPGIILPS
                     VRDLAVQLKVSRSTAARAIEELAAQGYVVAEMGSGTRVAPRLPGQLAGGPGLGQASIA
                     DKDAPLKEVELSRFAQRLVEREKLGAISATVKYTGPSLKEMPLSIWRDLIVRHCRAGA
                     EDLISYSPEPFGFPPLREAYASYLIRARAVKVTKERLITFASRNLRLDLLSRLLLQEG
                     DLVAMEEPGFAAAREQLLAAGCRLIPIPVDSQGLKVDQLDALQEPVKLVYVTPSHQSP
                     TGAVMSMIRRRQLLDYAARTGAYIVEDDYDSEFRYDGRPLPSLQGMDTNDRTIYLSCL
                     WNVLAPLTRLGFMVLPSAL"
     gene            complement(1091..2581)
                     /locus_tag="PKW73_08000"
     CDS             complement(1091..2581)
                     /locus_tag="PKW73_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNA73265.1"
                     /translation="MAIAINLEYKPYVALYRRLSDGLRKAILEGRLQLGEPMPSVREL
                     SESLKLSRATVLKAYEDLRTQGLVVSLSGSGTFVATTLPGDLGDLLQERFREPEPRLL
                     SAPDLHLSERGRQLYDVGYGQLQSHVHVSELNYGGTPHELSPAKEWKALMLKHCAIDQ
                     EDKVAEMVAPMGLMSLRQALAQYVMRRRSISLPDGPESICVVGSKQLRLELIGRMLLN
                     SGDVVAYEEPGYPENRNTLMAQGAVIAPIPVDEEGLSVKALRQLPTPPKLIYVTPSHQ
                     DPLAMVMSMPRRQELLEYSAAVGAFILEDDFDSEFRYGKPVLPALKALDHHDCVIYMS
                     SFWKVLYQSLRLTYLIFPRCLTKPGQLAKAYLERHLPLIDQLALADLINDGHLEKHLK
                     RCQRLLAHRRQSLILAITRYLRDYIEPFGEGGGTHLILNFKGGFSEDQIEAAAGRSGF
                     VIYNTNSYYLGQAPEAQFIAPFGSVDPDTIDGIVKSFAVNLATGAH"
     gene            2580..2717
                     /locus_tag="PKW73_08005"
     CDS             2580..2717
                     /locus_tag="PKW73_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73266.1"
                     /translation="MGLRCFDVEEAEGKSYEEKSYEEKSYKDSRDTLDMSLNRMSLND
                     K"
BASE COUNT          765 a          816 c          743 g          601 t
ORIGIN      
        1 ccagggccga gggcaaaacc atgaacccaa ggcgtgtcaa aggagcgaga acgttccaga
       61 gacaggagag atagatggtg cggtcatttg tatccatgcc ctgcaaagaa ggcagagggc
      121 gaccgtcata gcggaattcg ctgtcatagt catcttccac tatgtaagca ccggtgcgcg
      181 ctgcataatc gagcaactgc cgccgcctga tcatagacat caccgctccc gtgggagact
      241 ggtgagaagg tgtgacatag accagtttaa ccggttcttg cagggcgtct aactgatcca
      301 ctttcagccc ctggctatcc acaggaatcg gtatcaagcg gcagccggcg gcgagcaatt
      361 gctcgcgggc agcggcaaag cccggctcct ccatcgctac cagatcgcct tcctgcaaca
      421 acagccgcga tagcaggtcc agtcgcaaat tgcgcgaggc aaacgtaatc agacgctcct
      481 tggtgacctt gacggctctg gcgcgaatca aatacgaggc ataagcttcc cttaaaggtg
      541 gaaaaccaaa gggttcgggg ctgtaactga tgagatcttc ggcgcctgcc cgacagtgcc
      601 tgacaatcaa atcgcgccag atggaaaggg gcatttcctt caagctgggg cctgtatatt
      661 tgacggtagc gctgatcgct cccaattttt ctcgttccac caggcgctga gcgaatctgg
      721 aaagttccac ttccttcaag ggcgcatcct tatcggcaat ggaagcctgc ccgagacccg
      781 gaccgccggc caactgaccg ggcaagcgcg gtgccaccct ggtaccggag cccatctccg
      841 ccaccacata accctgagca gccaactctt ctatggctct ggcagccgtg gaacgcgaca
      901 ctttcaactg cacagccaga tcgcgcaccg aaggcaaaat aataccgggg ctaacgagac
      961 cttgcagaat tccctctttc atgacttcgg caatctgtct ggctaccgga acattagtct
     1021 tgaaatgctc gggaaaaacc agcctatcta tctgcatcat ccaaacctca agctatatcc
     1081 tacgtaaact ctaatgcgcg ccagtggcca agttgaccgc aaaactctta acgataccgt
     1141 caatggtatc agggtcgaca gaaccgaagg gagcaataaa ttgcgcctcc ggagcctgac
     1201 caagatagta agaattggtg ttatagataa cgaaacctga gcgtccggct gccgcctcga
     1261 tctggtcttc gctaaaacca cccttaaagt taagaatgag gtgggtgccg ccgccctctc
     1321 caaaaggctc gatatagtcg cgcaaatagc gggtaatagc cagaatcaag gactgcctgc
     1381 ggtgagccag caaacgctga caacgtttca aatgtttctc caggtgaccg tcattaatca
     1441 aatcagccag cgccaactga tcaatcaagg gcaaatgtct ctccagataa gccttggcca
     1501 gttgaccggg cttggtcaga cagcgcggaa agatcaagta ggtcagacgc agcgactggt
     1561 agagcacttt ccagaaggaa gacatataaa tgacacagtc gtgatgatcg agcgctttca
     1621 aggccggcag caccggctta ccgtaacgaa actcactgtc gaaatcatct tccagaatga
     1681 aggcgcccac cgctgctgaa tactccagca gttcctgccg cctgggcatg ctcatgacca
     1741 tggccagcgg atcttgatga gaaggagtaa cgtagatcag tttaggcggc gtgggcaact
     1801 gcctgagcgc cttgacactg agaccctctt catcgacggg aatgggtgcg atgactgccc
     1861 cttgcgccat aagagtatta cggttttcag gataacccgg ctcttcatag gccaccacat
     1921 cgccgctatt gagcaacatg cgcccgataa gctccaggcg caactgcttg gaaccgacaa
     1981 cacaaatcga ctcgggacca tcaggcaggg aaatggaacg ccgtctcata acatactgag
     2041 ccagagcctg cctcaggctc ataaggccca tcggtgccac catctcagcc actttatctt
     2101 cctgatcgat ggcgcaatgt ttgagcatca aagccttcca ctctttagct ggtgaaagct
     2161 catgaggcgt gccgccatag ttcagttccg aaacatgcac atggctctgc aactgtccgt
     2221 aacccacatc ataaagctgc cgaccgcgct cggaaagatg aagatccggc gccgaaagca
     2281 atctcggttc cggctcccga aaacgttcct gtaatagatc gcccaggtct ccgggcaagg
     2341 tagtggccac gaatgtaccg gagccggaaa ggctgactac cagcccctga gttctcaagt
     2401 cctcgtaagc tttcaagacc gtcgcccgcg acaacttcaa actttcggac agctcccgca
     2461 ccgagggcat cggctcgccg agctgcaacc gtccctctaa aatagccttg cgcaggccgt
     2521 ccgacagtcg tctataaagt gcgacataag gtttgtattc cagattgatt gcgattgcca
     2581 tgggattgcg ttgctttgac gttgaggaag ccgaagggaa gagctacgaa gagaagagct
     2641 acgaagagaa aagctacaaa gattcgaggg ataccctgga catgtcgtta aacagaatgt
     2701 ctcttaacga taagtgataa gaggccctta cacgatacca gatcaccgag cagttagaga
     2761 ctcttaccga tatgcggaaa ttgacgtgca ctactttttg ttttcctctt tgattctgcc
     2821 ggtagtgatt tcgggacaat ctgagacatt tctaaaaggc tcaaagaatc tacccgcaag
     2881 acgaaatgct tttaccaatc acggcgatac aaaagcaggt ttgcc
//
LOCUS       DAOQNZ010000389         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18121_length_2925_cov_3.700348, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000389 DAOQNZ010000000
VERSION     DAOQNZ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18121_length_2925_cov_3.700348"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKW73_08010"
     CDS             <1..399
                     /locus_tag="PKW73_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNA73267.1"
                     /translation="DGGAAYGAANVKEVHKLIAEADLLIVGFPVYNFSYSAALKNLVE
                     LTGKAWENKVVGFVSAAGGKSSYMSVMSIANALMLDFRCLVVPRFVYCDGDAFAGDEL
                     KDEAVARRLQELAGAASLLAETQSKFNRVL"
     gene            complement(396..1073)
                     /locus_tag="PKW73_08015"
     CDS             complement(396..1073)
                     /locus_tag="PKW73_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73268.1"
                     /translation="MSTSFGRSAPLILVLAFILGMYGFSQASRKADPKFSRDLKTTYP
                     RQAYQAMFYQKSDKGEGRVILSSDGKGHVRLKEVASVMRFSQPFKNSVGSERTENLFL
                     SVDRITLLDYLQGKKYIVFQHDKTIQESPLTNLGVGIFDEEMYKSSDAKRLEDTVIDG
                     TPVRGWLTTFPLTDAEQVEAWFSKQTGCLVFARGAGVTTNLLKYQKGSLPARDFLPPE
                     GYKKTKI"
     gene            complement(1084..1977)
                     /locus_tag="PKW73_08020"
     CDS             complement(1084..1977)
                     /locus_tag="PKW73_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73269.1"
                     /translation="MNAPALTLITRGGFPQFKISRGPAMVLAILISLNLAVGAQAQGF
                     GADRGETFGREPAPFGGEPGTFGRESAPFGREPDTFGRKTDTFGRESNTFGREPINQE
                     ETSQVVPTGVDQGNQVKPEQAGKEDETSAKSGKDEKKQGKWAIIQEGAGRFRYQMPNG
                     WALTRNPFVATDVLVKEAGDLNKSIVFEVRRGKPNVKARKVLEKQMQEKLKTSKVLEN
                     LVVTLGSDKKAGARLSLEGETKDFHVWQTAYLIDLGRGSYLVMTGSTPLTADKELADT
                     LARMADTIEATHQKISKIVRK"
     gene            2140..>2925
                     /locus_tag="PKW73_08025"
     CDS             2140..>2925
                     /locus_tag="PKW73_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA73270.1"
                     /translation="MRDVNAAAVQAGGSTCQPESASGSGIEVVNLCKTYKGGRVKAID
                     GLSLTVRPGEALGLVGPNGAGKTTLLGCLMGFLNYQSGTISIDGLAVDDLVTRSNVGY
                     LPERLTFDRWMTGRDFLHYHHELSGRPHKLQEAETEELLRRVDLEPSAWSKPVRKYSR
                     GMLQRLGFAQALIGKPRYLLLDEPASGMDPHGVLVVQELLQSLRAEGLTFLLNSHQLD
                     QIERSCDRVAFIQKGKVIKEENLRNRESSLSHTLIIKWLLDGAP"
BASE COUNT          603 a          753 c          809 g          760 t
ORIGIN      
        1 gatggtggtg ccgcctatgg tgccgccaat gtgaaagaag tgcataagct cattgcggaa
       61 gcagacctgc taattgtcgg ctttcctgtc tataacttta gctattcggc cgctttgaaa
      121 aacctggtgg agttgaccgg aaaggcctgg gagaacaaag tggtgggctt tgtctccgct
      181 gccggcggca agtctagcta tatgtcggtg atgagtatag ccaatgcctt gatgcttgat
      241 tttcgctgtc tggttgtacc gcgttttgtc tactgtgatg gggacgcctt tgccggtgac
      301 gaactgaaag acgaagccgt cgctcgtcgc ttgcaggaac tggccggcgc cgcttcttta
      361 ttggcggaga cccagagcaa gttcaaccgg gtgctctaga tcttcgtctt cttgtagcct
      421 tccggcggca gaaaatcgcg agccggcaag ctgcctttct ggtatttgag caagttggtg
      481 gtgacgcccg ctccacgagc gaagaccagg caaccggttt gtttggaaaa ccaggcttct
      541 acttgctcgg catcggtgag gggaaaagtc gtcaaccaac ctcgcactgg tgtgccgtca
      601 atgaccgtgt cttccaggcg tttggcgtcg gaagacttgt acatttcctc gtcgaaaatg
      661 cccactccca ggttggtgag agggctctcc tgaatcgtct tatcatgttg gaagacaatg
      721 tatttcttgc cctgcaggta gtcgagcaaa gtgattctat ctacggagag gaagagattt
      781 tcggtgcgct cgctgccgac actgttcttg aaaggctggg aaaaacgcat caccgatgct
      841 acttccttca agcgcacatg acctttgccg tcggaagaga gaatcactcg cccctcgcct
      901 ttgtccgact tctgatagaa catcgcttga taggcttgcc tggggtaagt ggttttcaag
      961 tcgcgggaga acttgggatc ggctttgcgc gatgcctggc tgaagccgta catgccgaga
     1021 atgaaggcaa gtaccagaat aagtggtgca gagcgcccga aagatgtgga catagtattc
     1081 ggcttacttc ctgactattt tcgagatttt ctggtgggtg gcttcgatgg tatcggccat
     1141 tctagccaga gtatcggcta attctttgtc cgcggtcaag ggtgtggagc cggtcatgac
     1201 caggtaagag cctcgaccca ggtcgataag atatgccgtt tgccagacat ggaaatcttt
     1261 ggtttcgcct tccagtgaga gtcttgctcc agccttttta tcgctaccga gggtgaccac
     1321 aagattttcc agaacttttg aagttttcaa cttctcctgc atctgttttt ccagaacctt
     1381 gcgcgcttta acattgggct tgccgcggcg cacttcaaaa actatcgatt tgttcaggtc
     1441 tcccgcttct ttcaccagaa cgtctgtggc aacgaaggga tttctggtca gggcccagcc
     1501 attgggcatc tggtagcgga accgaccggc tccttcctgg ataatggccc acttgccctg
     1561 ctttttctcg tccttcccgg atttggcgct ggtttcgtct tccttaccgg cttgctctgg
     1621 ttttacctgg ttgccctggt ctaccccggt aggtacgact tgtgaagttt cttcctggtt
     1681 tatgggctct ctaccaaaag tattcgactc tctgccgaaa gtatccgtct ttctaccaaa
     1741 agtatccggc tctctgccga aaggagcgga ctctctgcca aaagtgcccg gctctccacc
     1801 aaaaggagcc ggctctcttc caaaagtttc cccccggtcg gcgccaaaac cctgagcttg
     1861 agctcccacg gctaaattga ggctgattaa aatggccaga accatggccg ggccgcggct
     1921 gattttaaac tggggaaaac cccctctggt gatcagggtg agtgcaggag cgttcatgct
     1981 tcacttgtaa cataatcagg gtgatcctag actaaatttg gcctgaactt agttgttcct
     2041 agttgttcta gcaatttgga gcaggcgaca ttagaatgga aagactgcca gttgactacc
     2101 aggtaacaag tttttaccct tcctgcgagg gctcaattct tgagagatgt taatgctgcc
     2161 gccgttcaag ccggtggttc tacttgtcag cctgaaagtg ctagcggtag cggcattgag
     2221 gttgtaaacc tgtgcaagac ctacaagggt ggccgggtta aggccatcga tgggctcagt
     2281 ttaaccgtgc gacctggcga agccctcggt ctggtggggc caaacggtgc cggcaagacg
     2341 actttgcttg gctgtttaat gggtttcctc aattatcaaa gcggcaccat ttccatagac
     2401 ggtctggccg tggacgatct ggtcacccgg tccaatgtag gctatttgcc cgagcgcctc
     2461 acctttgatc gctggatgac cgggcgcgac ttcctgcact atcaccatga gctgagcggg
     2521 cgtccccaca aactgcaaga ggcggaaacc gaggaactct tgcgccgggt cgacctggag
     2581 ccttcggcct ggtctaagcc ggtgcgcaag tactcgcgcg gcatgctgca gcgccttggc
     2641 tttgcccagg ccttgattgg taaacctcgc tatttgcttc tggatgaacc ggcatccggc
     2701 atggatcccc atggcgtgct tgttgtgcag gaacttttgc agagtctgcg ggcggaaggt
     2761 cttacctttt tgctcaattc gcaccagctc gaccagattg agcggagctg cgatcgcgtc
     2821 gcctttattc agaaagggaa agtgatcaag gaagagaatt tgcgcaaccg ggaaagcagc
     2881 ttgagccaca ccctcattat taagtggctt ttggacgggg ctcct
//
LOCUS       DAOQNZ010000390         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18130_length_2924_cov_5.004531, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000390 DAOQNZ010000000
VERSION     DAOQNZ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18130_length_2924_cov_5.004531"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1285)
                     /locus_tag="PKW73_08030"
     CDS             complement(146..1285)
                     /locus_tag="PKW73_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA73271.1"
                     /translation="MKNKAAQSLPAKHYPGLAGKAYFNFGGQGVMSQPSLDAIFEAYQ
                     YVQREGPFSSKMFSWIGQQLNDTKELLAAVFGGSAHCFALTQNVTEAVNIVLWGLNWQ
                     AGDSIMITDCEHPGVVDAVNNLVRRLKLNLIEVAVQSLDGQELVDSIEKQISKDGRTR
                     LFVFSHVLWNSGKVLPLKEINNLCRQYGVLALADGAQSAGVLPLSLRDYDSDFYAFTG
                     QKWLCGPEGTGALYVKESLLGKLEPTYMGWRHHLHGSERGAEKYEIATSSFPLLAGLR
                     TALKCHQEAGDDRSRYVKIMANLNYLKSSLEELGVQTVAGAGASGLLSFKVPGKNHNQ
                     LVTDLESDGVYVRSIPEPDCLRASIHYLTESDDVDRLLAAVKKRL"
     gene            1427..1783
                     /locus_tag="PKW73_08035"
     CDS             1427..1783
                     /locus_tag="PKW73_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA73272.1"
                     /translation="MTHFGDYIRNKREQLQNRDAAAYSLRRVAAQIEIEPSYLSKIER
                     GLQPPPGEGTIIALARVLEEDPDVLLALGGKISEDLKRIIKQRPSLFAQLIRELKDMP
                     DHAVLHLVREVRDGNW"
     gene            1795..2853
                     /locus_tag="PKW73_08040"
     CDS             1795..2853
                     /locus_tag="PKW73_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73273.1"
                     /translation="MITLNQNQLVVSFPEVHEDAVLSLNFQRTLRIPDNDKVYPLPPG
                     LGNFPLVHVDDYRDEVPKSWLEHGGIIMPMYQSEAMWILFSASFSALRSSAYPMAIKI
                     STGKIDAVTGAPHTNGLHPHPQDYLVVPGQPWLDGYCVKKGMIRQFVAMPLGEGYTAE
                     EQITHKAEHGGVQIVVYPMKAEAYERLFPKVEREIWGSRSTGGWGAPGSMPPPPGSAK
                     MMMAAGGQAQSMGLAPGGRMKQEIYKDRFDINDWDTSKCSRCFVHIANSLTWREITGA
                     NPPHRPPTAVQYADYGLPWFDYYDENLKALSGSSKLANLKSVQALSQLKGEPIDNKPV
                     NTGSTVKLGPGQVREGDF"
BASE COUNT          645 a          763 c          821 g          695 t
ORIGIN      
        1 gggtggatca ggtgcgccac ggctttcaac agaactttta tgggggcatc ggcccggctg
       61 attggataga gttggctcgg gaaatcgctg cacagcttga agggaagcag cccatcacca
      121 acgctctggt cctcaagcac ttttgctaga gtcgtttctt gactgcggcc agcaggcggt
      181 ctacatcatc gctttcagtt aaatagtgaa tggatgcgcg caagcagtcg ggctctggaa
      241 tgcttctgac atatacaccg tcgctttcca gatcggtcac gagctgattg tgatttttgc
      301 cgggaacctt gaagcttaga agtccagagg cgccagcacc ggctacagtt tgcaccccca
      361 gttcttccag gctcgatttc agataattga gattagccat gatcttcacg tagcggcttc
      421 gatcatcgcc agcttcctga tggcacttga gggctgtgcg cagaccggcc agaaggggga
      481 aagaagaggt ggctatctca tatttttctg cgccgcgctc actgccgtgt aaatggtggc
      541 gccaacccat ataagttggt tctagcttgc cgagcaggct ttcttttacg tagagggcgc
      601 cggttccctc cgggccgcag agccattttt gaccagtgaa ggcgtaaaaa tcgctgtcgt
      661 agtcgcgcag gctgagagga agcactccgg ctgattgggc gccgtcggcg agagccagaa
      721 ctccatactg tctgcagagg ttgtttattt ctttgagcgg taagaccttg ccgctattcc
      781 agagcacatg gctgaaaaca aagagccgtg tgcgcccatc tttgctgatc tgcttttcaa
      841 tgctgtctac aagctcttgc ccgtccagac tttgtaccgc tacctcgatg agattgagct
      901 tcagccgccg cactagatta tttaccgcat ccaccacgcc ggggtgttca cagtcggtga
      961 tcataatgct gtcgccggcc tgccagttta gtccccagag tacgatattc actgcttcgg
     1021 tgacgttttg ggtgagggca aagcaatggg ccgacccgcc gaagaccgca gcaagaagct
     1081 ctttggtgtc gttcagctgc tgccctatcc agctgaacat tttgcttgag aagggaccct
     1141 cgcgctgtac atattgatag gcctcaaaaa tggcgtcgag gcttggctgg ctcatgacac
     1201 cctgcccacc aaagttgaag taggcttttc ccgccagacc gggatagtgt ttcgctggta
     1261 aggattgtgc tgctttgttt ttcataagtc cccgagagta tgaatcttac cgcatggcgg
     1321 tgatttgagg gccataactt gcggttgcgg ggcattaacg gggcgttcgc ctgaaccctc
     1381 agggggtggc ttgacaagtg tgtagtgtgt agtagataat acacacatga ctcatttcgg
     1441 cgactacatt cgaaacaaga gagagcagct ccagaacaga gacgccgctg cttattctct
     1501 gcgtcgcgtg gctgctcaga ttgaaatcga gccatcttat ttgagcaaaa ttgaaagagg
     1561 tttgcagcct ccgccgggtg agggcaccat cattgctcta gcccgagtgc tcgaagaaga
     1621 tcccgatgtt ttactggctc tggggggaaa gatttcagaa gacctgaagc gaatcattaa
     1681 gcagcgcccc agtctttttg ctcaactgat tcgcgaatta aaggatatgc cggaccatgc
     1741 agtgttgcat ctggtgcgtg aagtacgcga cggcaattgg taaggagaag atggatgata
     1801 actctcaatc agaatcaact ggtggtgtca tttcccgaag tgcatgaaga tgccgtgctc
     1861 agcctcaatt ttcagcgtac cctgagaatt cccgataacg acaaggtcta tcctctgccg
     1921 cccggtctcg gcaacttccc ccttgtgcat gttgatgatt atagagatga ggtgcccaaa
     1981 agctggcttg agcacggcgg catcatcatg cccatgtatc agtccgaggc catgtggatt
     2041 ctcttttctg cttccttctc cgctttgaga agttccgcct atcccatggc cataaagatt
     2101 tccaccggca aaattgatgc ggtgaccggc gcccctcaca caaacgggct gcatccccat
     2161 ccgcaagact atctggtggt gcccgggcag ccctggctgg acggctactg tgttaagaag
     2221 gggatgattc gtcagtttgt ggctatgcct ctgggagagg gctatacggc tgaggagcag
     2281 attacacaca aagccgagca tggcggtgtg caaattgtcg tctatcccat gaaggcagag
     2341 gcgtacgaaa ggctgtttcc caaagtcgag cgagagatct gggggagcag gtctactggt
     2401 ggttggggcg ctcccggctc aatgccgcct ccccccggca gtgcgaaaat gatgatggcg
     2461 gccggtggtc aggcgcagtc catggggctg gctcccggtg gtcgaatgaa gcaagagatc
     2521 tataaagacc gatttgacat aaacgattgg gatacgagta agtgcagtcg ctgctttgtt
     2581 cacatcgcca attccctcac ctggcgcgag attaccggcg ccaatccccc gcacagaccg
     2641 cccacggctg tgcaatatgc cgactacggg ctgccctggt tcgattatta tgacgagaat
     2701 ctcaaggccc tctccggctc aagcaagctg gccaatctca aaagcgtgca agctctatct
     2761 caacttaagg gagagcccat cgacaataag ccggtaaata caggcagtac ggttaaactc
     2821 gggcccggac aggtgcgaga aggcgatttc taaagccttt agctcaggtg tttgcaggcg
     2881 tcggtcggct ctcagtcggc cggcgcctgc ggcgctgccg tctg
//
LOCUS       DAOQNZ010000391         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18147_length_2923_cov_3.115411, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000391 DAOQNZ010000000
VERSION     DAOQNZ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18147_length_2923_cov_3.115411"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKW73_08045"
     CDS             complement(<1..517)
                     /locus_tag="PKW73_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73274.1"
                     /translation="MTQLPVVRDKFSVEKCLSDGWKGMISHFLPLFGVMVVSFLVMAM
                     ATLPTAVVIVCHIMKLPLPAIADVFLSVLGMVAGFVVGPMMQMGMLRACLKVIDNEMP
                     SPKDLFVCWPYFWQFFSAGFLMKCMRFPAYLCFIFPGIWLDLSFRFYEYFVVDRGMGP
                     IQAFRASNQVTK"
     gene            complement(526..2634)
                     /locus_tag="PKW73_08050"
     CDS             complement(526..2634)
                     /locus_tag="PKW73_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA73275.1"
                     /translation="MTLHSEKKTIFSLAVAFLLLAVASLLSMMGFGQFHGDRWLETRQ
                     SSVKELQSIGSAYSMVLVNSQRYVVSGEGQHLSLFNQSLEDLKLHMGSLQALDGGQYG
                     RTERVRRLSNELALSLGEIDLDVKLRQDKGSSPAQSLLLTNREMRDADGILKRIVAME
                     QEESAAIQAFFSGDVKARGNSLPLVAFVFACLSFVVALVELFGNRRDFEFDIADEARL
                     PGLDAVRPLSPAYSGGASGAYSSGDNGNGNGNGNGYYGNGTGELSQDDLTGLRRDLAG
                     ALSQLERLANVDYLTDVLNVRGLEQVLRIEDNRSGRSGEPLIAMLINCDNLKKVNEGL
                     GHGTGDVILKETAKRILGILRPSDHVARVGGDEFLVLLPDTSLPYGMRVAERIRSGIS
                     DAPMQGANDVVPVTASIGVVSLPAKIASVQEAVNLARAALKRSKAAGKNKVTVAEESP
                     LTKGSRVTQRDIVEQLLDAGEFRTVYQPIIALASEKVEGYEVLSRGPDGAFESPADFF
                     RICVENNILTTVDLQCLRLCLAAPRDLEENARFHVNLFPSTLLETPVDKLIELFPADR
                     SHIRYCVEISEQEFISEPAYLRDHVNALKHAGILVAIDDVGFGRSSLETLILLEPDLV
                     KVDRKYVAGLSNEPAKARLLRRLANVAKSLGAQIIAEGIENREDLPLLMEMGIDYGQG
                     FLWGELLGKLPAREDMVKKG"
BASE COUNT          779 a          820 c          729 g          595 t
ORIGIN      
        1 ccttggtcac ctgattgctg gctctgaagg cttgaatcgg ccccatgccc cggtctacaa
       61 caaaatactc atagaagcga aaggacaggt ctaaccagat gcccggaaaa atgaaacaaa
      121 gataggccgg gaatcgcatg cacttcatca ggaagccagc cgagaagaac tgccagaaat
      181 atggccagca gacaaataga tctttcgggg aaggcatctc attatctatc accttcaaac
      241 aggcgcgcag catccccatc tgcatcatgg gtccaaccac aaaaccggcc accataccaa
      301 gcaccgaaag aaagacatcg gcaatggcag gcaagggtaa cttcatgatg tggcaaacga
      361 taaccacagc cgtaggcaaa gtggccatag ccatgaccaa gaaggagaca accatgacgc
      421 caaagagagg taggaaatga gaaatcatac ctttccagcc gtcggaaagg cacttctcaa
      481 ccgagaactt atcccgcaca actggtagtt gcgtcatgtt tcgagctacc ccttcttgac
      541 catatcttcg cgtgccggca gtttaccgag caattcaccc cagaggaagc cctgaccgta
      601 atcgatgccc atctccatca agagcggcaa atcttctcta ttctctattc cttcagcaat
      661 aatctgcgct cccaggctct tggcaacgtt ggcaaggcgc ctgagaagtc gggctttagc
      721 cggctcattg gagagaccgg ctacatactt tctatccacc ttcaccagat caggctccaa
      781 caaaatcaat gtctctaagg agctgcgtcc gaagccgaca tcatcgattg ccacgagtat
      841 accggcatgc ttgagagcat ttacatggtc gcgcaagtaa gccggttcac tgatgaattc
      901 ctgctcggag atctcgacac agtagcggat atgggaacgg tcggccggga agagctcaat
      961 gagcttatct acaggcgttt caagcaaggt cgaggggaag aggttgacat ggaaacgagc
     1021 attttcctct aaatctctcg gtgctgccag acagaggcgc agacactgca aatctactgt
     1081 ggtgagaata ttgttctcca cgcaaatacg gaagaaatcg gcagggctct cgaaggctcc
     1141 atccggccca cgactcaaga cttcatagcc ttctactttc tcgcttgcaa gagcaataat
     1201 gggctgatag acggtacgaa actcgccagc atccaagagc tgttccacaa tgtcgcgctg
     1261 ggtaacgcgt gaacctttgg tgaggggaga ttcttccgcc accgtaactt tattcttgcc
     1321 ggcggctttg ctgcgcttaa gagcagcccg ggctagattg acggcttcct gcacggatgc
     1381 aattttggca ggtagactga cgactccaat ggaagcggtg accggtacca catcgttggc
     1441 gccctgcatg ggggcatcgg aaatgccgct gcgaatacgc tcagccaccc tcatgccata
     1501 aggcaggctg gtatcaggca gaagcacaag aaactcgtca ccgcccactc tggccacatg
     1561 atcggaaggg cgcaaaatac caagaatgcg cttggccgtc tctttcaaaa tgacgtcgcc
     1621 ggtgccgtgg ccaagccctt cattgacctt cttgaggttg tcgcagttga tgagcatggc
     1681 gatgagtggc tccccggaac ggccggagcg gttgtcctcg attcgcaaaa cctgttccag
     1741 accacgcacg ttcaagacat cggtaagata atctacatta gccaggcgct ccagttggga
     1801 aagcgcaccg gccaaatctc ggcgcaatcc ggtcaggtca tcctggctga gttcaccggt
     1861 accattgccg taatagccat tgccgttacc attgccgttg ccgttatcgc cagagctgta
     1921 ggcaccggaa gcgcctccac tgtaggcagg cgagagcgga cgcacggcat ctaaaccggg
     1981 cagacgggcc tcatcggcaa tatcaaattc aaagtcgcgc cggttgccga agagttcaac
     2041 cagagctacc acaaaactga gacaggcaaa aacaaaagcc accagaggca aactattccc
     2101 cctggctttt acatccccgg agaagaaggc ctggatagca gcgctctcct cctgctccat
     2161 agccacgatt ctcttgagaa taccgtcggc atcgcgcatc tcccggtttg tcagaagcaa
     2221 actctgggca ggactggaac ctttatcctg gcgcaattta acatcgagat caatctcccc
     2281 gagactgaga gccagctcat tggaaaggcg acgtacccgt tcggtgcgtc cgtactggcc
     2341 gccatctaga gcctgcaagc tgcccatatg cagcttcaag tcttccaggg actgattaaa
     2401 gagactcagg tgttgccctt cgccactgac cacatagcgc tgactgttaa ccagaaccat
     2461 actgtaagcc gaaccgatgg actgcagttc cttcacggag ctctgcctgg tctccagcca
     2521 tcgatcaccg tggaattggc caaaacccat catggacaac aaggaagcca ctgccagaag
     2581 taaaaaggcg acggcaaggc tgaatattgt cttcttttca gagtgtaagg tcataagaaa
     2641 gcgccgtaag gggcgaccat gtggggatac cgtccgataa acaaagagcc gggaagccac
     2701 ctatctcata cacaaaaacg agcagatcta atcataaata gatggattga ccctatagcc
     2761 gttacacgga cgcaccaaac ggctagttgc caaaagccct gtagaatccg ccaaacggcg
     2821 agagctcaac cttaataccg ggattatcct tcttgaactg ttcttcagcc tcagccgaga
     2881 cgttgcaacc ggcgatattg atagtcttaa gctgcttgcg ccc
//
LOCUS       DAOQNZ010000392         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18210_length_2917_cov_4.067785, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000392 DAOQNZ010000000
VERSION     DAOQNZ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18210_length_2917_cov_4.067785"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PKW73_08055"
     CDS             <1..607
                     /locus_tag="PKW73_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNA73276.1"
                     /translation="STAEPNPPGARLRRGFSGKAKAAACMWAALVLAGGAGWLASEAK
                     ERTPDGECRSLGDELGGDPEFEKALVKHMEKKFFARINATPEQRSKIATLVESKMDAN
                     RGKREALRSGLKSLVELVRNPQAKDEEITARAHELRNMHQALMDDRLNTFLAVRALLD
                     DEQKSKLGKRIAKVMEGQGRPRLGLLKQINLKEAVDSDEEG"
     gene            623..1225
                     /locus_tag="PKW73_08060"
     CDS             623..1225
                     /locus_tag="PKW73_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA73277.1"
                     /translation="MSEINTERDRELVQKSLKGDAKAFEALVRHYQVLVLTVVHRTLQ
                     DQEAARDITQETFVKAFRALKTFDGKRSFKTWLLAIASNACIDYLRSRSSKGALSLEK
                     ILEEEPYLEQSFATAHKTSPEEEAEMNLFITKLSWALSLIPLRYRQSFILRYQFDLTY
                     EEISQVMNENENTVRTLLHRAKERLRKLILETKNPFPNLE"
     gene            1233..1739
                     /locus_tag="PKW73_08065"
     CDS             1233..1739
                     /locus_tag="PKW73_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73278.1"
                     /translation="MNNQSEKCLLVQNMLDSFSEKLVDSRIKLRQKEGSQAPMLEDEA
                     QEHLALCEDCLNYKIANAALMETASALPMLPYDESLTLSIMKDLAPDFHLQSTAENLS
                     APTTSQDKSIMLLLLSFAVFALLLPLEAGDSPWSILSWSLALSLVILLKPLLKKHAEN
                     ENLPYARV"
     gene            1726..2487
                     /locus_tag="PKW73_08070"
     CDS             1726..2487
                     /locus_tag="PKW73_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73279.1"
                     /translation="MPEFRRHGAIMALMVAAFLALPLWLTPCADGVAGHTQPAWKSAL
                     YSTREALQNSEPILQSKEIRFPFIYFRQALNHKWQPRYFFPFLVFAALSFSFVAPGSL
                     KAAINNCRSNLPGCLAFSFIFTVLTMILAKAAFAAPALEPLGVVVMGLVELAYILGLA
                     VGTQSLAESLTEKCSARLVGNTASEPDGKGGRQAILKKDRLINVAVVILVAALLSTLT
                     LIPDLGRLPRLGNRLLVVVAMLGLGGLLKAKYRQN"
BASE COUNT          780 a          773 c          751 g          613 t
ORIGIN      
        1 gtcaacggca gagccgaacc cgccaggcgc ccggctacgg cgcggcttct ccggcaaagc
       61 gaaagcggcc gcctgcatgt gggcagccct ggtgctggcc ggcggagccg gctggctggc
      121 ctccgaagcg aaggagcgca cgccggatgg ggaatgccga agcctgggcg atgagcttgg
      181 cggcgacccg gaattcgaaa aagccctggt taagcacatg gagaagaaat tctttgccag
      241 aatcaatgct acgcctgagc aaagaagcaa aattgcaacc ctggtggaaa gcaaaatgga
      301 tgccaatcgc ggcaaaaggg aagccctgag aagcggtctc aaatcgctgg ttgagctggt
      361 cagaaatcca caggcaaagg acgaagaaat aacagcccgg gcccacgaac tgagaaatat
      421 gcatcaggcc ctcatggacg acagattgaa caccttcctg gccgtgcgag cgctgctcga
      481 tgatgagcaa aagagcaaac tgggcaagag gatagcaaag gtcatggaag gacagggtag
      541 acccaggctt ggcttgctca agcagatcaa tctgaaagaa gctgtagact cagacgaaga
      601 aggctaagtc agagtagaga cgatgtctga aatcaatacg gaaagagaca gagaactggt
      661 tcagaagagc ctcaaaggtg atgcaaaagc ctttgaggct ctggtcaggc actatcaggt
      721 cctggttctg accgtagtgc accgcacctt gcaggatcaa gaagcggcca gggacatcac
      781 ccaggagacc ttcgtaaaag cctttcgcgc cttgaaaacc tttgacggca aacgctcctt
      841 caagacctgg cttttagcca tcgccagtaa tgcttgcata gactatctca gatccagaag
      901 cagcaaaggt gctctatccc tggagaaaat tctagaagaa gaaccatatc tggagcaaag
      961 ttttgccacc gcccacaaga cttccccgga agaagaagcc gagatgaacc tcttcatcac
     1021 aaagctcagc tgggccctct ctttgatccc cttacgctac agacagtcct tcatcttgcg
     1081 ctaccagttc gacctcacct atgaagagat aagccaggtc atgaatgaaa atgagaatac
     1141 ggtgcgcaca ctgttgcata gagcaaaaga gagactgcgc aagctaattc ttgaaacaaa
     1201 aaaccccttc cccaatttag aatgatagag ccatgaataa ccaatctgaa aagtgtctgc
     1261 ttgtacaaaa tatgctcgat agctttagtg aaaagctggt cgacagccgc atcaagctgc
     1321 gtcagaagga aggctcccag gcgcccatgt tggaggatga ggcccaggaa catctggccc
     1381 tttgcgaaga ctgcctcaat tacaagattg ccaacgccgc cctgatggaa acagccagcg
     1441 cactgccgat gctaccatat gatgaaagcc tcactctcag tatcatgaaa gacctggccc
     1501 cggattttca cttacagtcc actgcggaaa atctctctgc cccgacaacc agccaagaca
     1561 aatccatcat gctcctctta ttgtcctttg cggtttttgc ccttctactg cccctggaag
     1621 ccggtgattc gccctggagc atcctcagtt ggagcctggc tctaagcctg gtcatcctgc
     1681 tcaaacctct tttgaagaaa catgcggaaa acgagaacct accctatgcc agagtttaga
     1741 cgtcacggag caattatggc tttgatggtg gccgctttcc tggctctacc gctctggctt
     1801 acgccctgtg ccgatggtgt tgccggacac actcaaccgg cctggaaaag cgctctatac
     1861 tccacccggg aggccctgca aaactcggaa ccaattttgc aaagcaagga aatccgcttt
     1921 cccttcattt attttcgcca ggccttgaac cacaaatggc agccgcgcta tttctttcct
     1981 ttcctggttt tcgctgccct ctccttcagc tttgtcgctc ccggcagctt gaaagcagcc
     2041 attaacaact gccgcagcaa tctgcccggc tgcctggcct tctccttcat ttttacggtt
     2101 ttgaccatga ttctggccaa agccgcattt gcggcgccag ctctggaacc cctgggggtg
     2161 gtagttatgg gtctggtgga attggcttat attctcggtc tggcagtggg cactcaaagc
     2221 ctggctgaaa gcctgacgga aaaatgctcc gcccgcctgg tggggaacac ggcctcagag
     2281 ccggacggca aaggcgggag gcaagccata ctgaagaaag accgtctgat aaatgtcgca
     2341 gttgtgatcc tggtggcagc cttgctttcc acccttaccc tcatccccga tctgggacga
     2401 ctaccgaggc tgggaaaccg cctcctggtg gtggtggcga tgctcgggct gggtgggctg
     2461 ctgaaagcaa aatacaggca aaactaaccg ccggcatagg tgcaaccact gcctttggaa
     2521 aataatcagc taagcgacgc tcaggactcg tcgggaccgg tatagggctc aaagaaagcc
     2581 tttttggcgc ctagtcccaa gaagattaat aagctaaccc cgaggacgat tcctacaaag
     2641 acttcgggag gggtgtcaaa ggttggttgc ataacggttg agcatgctcc ggcaaggaat
     2701 agtcaagttc agtcaaattt gcaagttgaa aacactatct gcaattaata tatctaagtt
     2761 agcacgattt tctcaataga gacctatatt gagctgctta atactcaaag tagtagcaaa
     2821 aagcaggttc gagagagtca aaccgctgcc ttgagggaca gatgataaac atttgacctg
     2881 aaagttatga ggtttctaag aaacggctca ccccttg
//
LOCUS       DAOQNZ010000393         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18302_length_2908_cov_3.471434, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000393 DAOQNZ010000000
VERSION     DAOQNZ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18302_length_2908_cov_3.471434"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKW73_08075"
     CDS             complement(<1..234)
                     /locus_tag="PKW73_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73280.1"
                     /translation="MLRKVRNKKGQGAAELVGAIVCLVPVILVVIDLGVVAIGAGIND
                     AVCRDAARAAASGPPSELTLAENRVVTAGKAPHE"
     gene            414..983
                     /locus_tag="PKW73_08080"
     CDS             414..983
                     /locus_tag="PKW73_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73281.1"
                     /translation="MKVSQKPGPSKGARNRSGHAMSEFAAALIVLISFIFMPLLNISI
                     IPVRYLMAQGVLNELSHRVVLCDKRSEASAMFAGDGSVWSKSLLSKCGVTVSNPKLTL
                     TIFGKEPADKLVVLAGDTIPAEWLPDGAKAPNLYSMELSADCAIAPFVECTPGLPGFT
                     SPITMRLKAASPWENFGRDPATQEYYVNE"
     gene            1044..1826
                     /locus_tag="PKW73_08085"
     CDS             1044..1826
                     /locus_tag="PKW73_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73282.1"
                     /translation="MIGYSYGGLFFEHNRLQASANEIALAGARKFNDMDRIGQMNNMI
                     ARNRQLVFSGRHQLDECVSKAPGLKDLATQLLDESRDSAKTLESQRLQLAVVARKEAK
                     DAVLNKFVQVQGSYAMFLPWMKVEAPSLSGWQCGRAAKVDSNVEALMAVDDLVDHDKA
                     QDFIVGSGMKLYKHDIDAHLPGSDNDLHFRLSSLPAPVKKTISPARIILNGNFETIAA
                     DELPSVCRVKLWLNVGTGLGPKAEGTMSCVGTASATGGMSQQ"
     gene            1874..2230
                     /gene="arsC"
                     /locus_tag="PKW73_08090"
     CDS             1874..2230
                     /gene="arsC"
                     /locus_tag="PKW73_08090"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNA73283.1"
                     /translation="MAKDTITVYEKPTCSKCREAAKLLRDNGVEFQKVNYYTQPLTEA
                     KLTELLAKMKLKPRDVMRTGEAVYKELKLSGSSHSDSELVKLMVKHPDLLQRPIVEKG
                     SRAVLGRPTENIEALL"
     gene            complement(2234..>2908)
                     /locus_tag="PKW73_08095"
     CDS             complement(2234..>2908)
                     /locus_tag="PKW73_08095"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNA73284.1"
                     /translation="LDAGHGGNDTGAVVQGVKESEVTQALTKDLAQALRERNVSVELS
                     NPNGLQGGPKMPLASRVFFGNSQFKTNEETRLERANREVGGIQGRDQDKACYADAFVS
                     VHANFEGGKGKGDGSQRGFATYMYKGKEKDGTRDLANSIQGAVVEGRDRGLDVVDGRV
                     NDKQAFKVMREAQAPAVLLETGYLTNNRDRANLADPEYRKRLAGHMADGIARFLNGRP
                     ERCFNK"
BASE COUNT          589 a          770 c          812 g          737 t
ORIGIN      
        1 ctcatggggg gctttgccgg ctgtcaccac tcgattttcc gccagggtca gttcgctggg
       61 ggggcctgag gccgctgctc tggcggcatc gcggcatacg gcgtcgttga tgccggcacc
      121 gatagccacc actcccagat ctataactac cagtattaca ggtaccaggc aaactatggc
      181 tccgaccagt tcggccgctc cctgtccttt cttgttgcgt acttttctta gcatggtttt
      241 ctccccgtta tcctgcctcc acggggacgg cgctgttttt aggggagttg cccggggttt
      301 tgcctgaagt tttgcggaat tagtgcggtt ttatctcttt tgtggcggct taaggcggag
      361 tggtcctaaa aatttcgcca tggcggtcag gtgaatatca gcgaggaacg catatgaaag
      421 taagtcaaaa accaggacca agcaagggcg ccagaaatcg aagcggccat gccatgagcg
      481 agtttgccgc ggcactgatc gttctcattt ccttcatttt tatgcccctg ctcaatatta
      541 gtattattcc ggtacgctac ttgatggctc agggtgtttt aaatgagctt tcccacaggg
      601 tggtgctctg cgacaagcgc tcggaggctt ccgccatgtt tgccggcgac ggttcggtct
      661 ggtccaagtc acttctttct aagtgcggcg tcactgtctc caatccgaag cttactctca
      721 ccattttcgg taaggagcct gcggataaac tggtggtttt agccggtgat accattcctg
      781 ccgaatggtt gccggacgga gccaaggcac ccaatcttta tagtatggaa ttgtcggccg
      841 attgtgccat cgctcctttt gtggagtgca caccaggact gcccggtttt accagtccca
      901 tcaccatgag actgaaagcc gcttctccct gggaaaattt tggtcgcgat cctgccactc
      961 aggagtatta cgtcaatgaa taggaagcgg ggctcggtct tagttctggg ttcggtggtc
     1021 atgggtcttc tggctttagc tgtttgatcg gctattcata tggaggtctg ttctttgagc
     1081 ataaccgcct gcaggcttcc gccaatgaga ttgctctggc cggtgcgcgc aagttcaacg
     1141 acatggatcg catcgggcag atgaataata tgattgccag aaaccgacaa ctggtatttt
     1201 ccggtcgcca ccaacttgat gagtgcgtta gcaaagcgcc cggactaaag gatcttgcca
     1261 ctcagttact ggatgaatca agagattcgg ccaagaccct ggagagccag cgtctgcaac
     1321 tggcggtggt ggcacgcaaa gaagccaagg acgccgtgct taataagttt gtgcaggtgc
     1381 agggcagcta tgccatgttc ttaccctgga tgaaggtgga ggctccctcc ctttccggct
     1441 ggcagtgtgg tagagccgct aaggttgatt ccaacgtgga agcgctcatg gctgtcgatg
     1501 atcttgttga tcatgataag gctcaggatt ttatcgtcgg cagcggtatg aaactctaca
     1561 agcatgatat tgatgcacat ctgcccggca gtgataacga cctgcatttt cgcctcagca
     1621 gtttgccggc gccggtgaag aagaccatta gccccgcccg cataattctg aacggtaact
     1681 tcgagaccat tgccgctgat gaattgccct cggtatgccg ggtgaagctc tggctcaatg
     1741 tcggtaccgg ccttggtccc aaagcggaag gcaccatgag ctgtgtcggt acggcaagtg
     1801 ccaccggcgg tatgagccag caataaattc ggcttgcggc tggtaaaata gcctgcaagt
     1861 tcgagaggtt gccatggcta aagatacgat taccgtttac gagaaaccca cctgctcaaa
     1921 atgcagagaa gccgccaagt tgctgcgcga caacggtgtg gaattccaga aagtaaacta
     1981 ttacacccaa ccattgactg aagctaagct gacggaattg cttgccaaga tgaagcttaa
     2041 accacgggac gtaatgcgca ccggtgaggc tgtttacaag gaattgaaac tttccggtag
     2101 tagccacagt gatagtgaac tggtgaagct tatggtcaaa caccccgatc tgctgcagcg
     2161 ccccattgtc gagaaaggca gccgtgctgt gctggggcgg cccaccgaaa atatcgaagc
     2221 gctcttgtaa gggctattta ttgaagcagc gttccggtct gccgttcaag aagcgggcga
     2281 tgccgtcggc catgtggccg gccaggcgct tgcggtactc ggggtcggcc agattggcgc
     2341 ggtcgcggtt attggtcagg tagcctgttt ccaacagtac cgctggtgcc tgggcttcgc
     2401 gcattacctt gaaggcttgc ttgtcgttta ctctgccgtc caccacatct aagccgcggt
     2461 cccggccttc caccactgct ccttgaatgc tattggccag atctctggtg ccgtcttttt
     2521 ccttgccttt atacatgtag gtggcaaagc ctctctggct gccgtcacct ttgcccttgc
     2581 cgccctcaaa attagcgtgc actgagacaa aggcgtcggc atagcaggcc ttgtcctgat
     2641 ctctaccctg gatgccgcct acttcccggt tggctctttc caatctggtt tcttcattgg
     2701 ttttgaactg gctgttgccg aagaaaacgc gggacgctaa gggcatcttc ggtccacctt
     2761 gcagtccgtt ggggttggac aattccaccg atacgttgcg ctcgcgcagg gcttgtgcca
     2821 ggtcttttgt gagagcctgg gtgacttcgc tttccttgac gccttgcacc acggcgccgg
     2881 tgtcgttgcc gccgtgtccg gcatcgag
//
LOCUS       DAOQNZ010000394         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18330_length_2906_cov_3.566819, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000394 DAOQNZ010000000
VERSION     DAOQNZ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18330_length_2906_cov_3.566819"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKW73_08100"
     CDS             <1..285
                     /locus_tag="PKW73_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73285.1"
                     /translation="PIFMQLLSETHTDSLVPLAKRIDTWSKVLDQEADRLSRQNTQDA
                     NELMADECNSGGFTLADESHRKKWGRNNILVAVWLFRNAELYADEPSSAL"
     gene            complement(282..1604)
                     /locus_tag="PKW73_08105"
     CDS             complement(282..1604)
                     /locus_tag="PKW73_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA73286.1"
                     /translation="MDTRQKKIAIIGAGVAGMVAALELSKGGAKVVVFEKSSKAGGRA
                     QTTHKEGYYLNFGPHALYAGGLAHRYLQNNGIDYRGAAPPLNRENLAIFAGEVYELPV
                     NLSSLFRATYLNWSEKIELARFFRRLPHLDLDKIMSVNLAQWLKLNVKRTRVRQTIET
                     FVRLSTYANSPQELSAGAAFAQVILANQGVLYLDHGWQTIVESIQRKLNSESTSFRYG
                     AQVQSVKQLADAVEILVDEETFLFDAVLLCIPPQQVEKLLPDALSGQALSTCRAACLD
                     VCLKRLPRPKHTFALGVDEPLYYSVHSTTAKLADGEGVLIHLAYYLEPQDNRDDHEER
                     LLRLLEQLQPGYEQELVYKRYLPHMVASYGLPRADHAGAAGLKSPRLKGYPSIFVCGD
                     YVGSDWQLVDCAVNSSLEAVQTVLASRDEGVYGRWQENAASATVESQG"
     gene            1706..2194
                     /locus_tag="PKW73_08110"
     CDS             1706..2194
                     /locus_tag="PKW73_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNA73287.1"
                     /translation="MSQGIEWSIHCCALLALLPPKRTLRIEKIAQFYDLPVPYLRKHF
                     QDLSRAGILKTTSGPKGGYRLAREAKDVNLLHIYEAIEGPEHAFRCNEIRQNGPNTCG
                     QKVYPAACNIATSMWKAEEAFRRELEKVSLEDIVKTAALQISPERRQKSAQWLEEALS
                     NQ"
     gene            complement(2265..2759)
                     /locus_tag="PKW73_08115"
     CDS             complement(2265..2759)
                     /locus_tag="PKW73_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73288.1"
                     /translation="MAQCEFCGKPPIGETKHCVRCSMLQVVLKQACALGLPWRKVCAP
                     LMAAQKVTVSDVEAQMRLHVDKCSLEISADGKSVESYKKRGILYCQLDEFDRAWDDAE
                     AAMAIDEREPYIYLIRSVVYSSRKQYPEAVEECNKAIALDPSLEYAKILKQGASEAQQ
                     SSLS"
BASE COUNT          734 a          816 c          680 g          676 t
ORIGIN      
        1 ccaatattca tgcaattgct ctccgagact cacacagatt ccctggtacc actcgccaag
       61 agaattgata cctggagcaa ggttctggat caagaagcag accgactctc caggcaaaac
      121 acccaagatg ctaacgaact aatggcagat gaatgtaatt ccggcgggtt tactctggct
      181 gacgaatccc acagaaaaaa atgggggcgc aacaacatac tcgtagctgt ctggctgttt
      241 agaaatgcag aactttacgc tgatgagccg tccagcgccc tctagccttg tgactccact
      301 gtcgcggagg cggcattttc ctgccacctt ccataaactc cttcatctct tgaagcgagc
      361 actgtttgca ccgcctccag actgctattg acggcgcagt ctacaagttg ccagtcgcta
      421 ccgacataat ccccacaaac aaaaatggaa ggatagcctt tcagcctggg actctttaga
      481 ccggcagcac cagcatggtc ggcacgaggc aagccatagc tggctaccat gtgcggcaaa
      541 taccgtttat atacaagttc ttgctcataa cccggctgca actgctccag caaacgaagc
      601 aatctttcct catggtcatc tctattatct tgaggctcca gatagtaggc aagatgaatg
      661 agcacccctt caccatccgc cagttttgca gtggtggaat gtacggaata atagagcggc
      721 tcgtctacac ccagggcgaa ggtatgcttg ggtcgaggca gacgcttcaa gcaaacatcc
      781 aggcaggcag cgcgacaggt agaaagagct tgcccactga gtgcatccgg tagcagtttc
      841 tccacttgct gaggaggaat acacagcaat accgcatcaa acaaaaacgt ctcttcgtct
      901 accaaaatct ccaccgcatc ggccagttgt ttgacagact gaacctgagc gccatatcgg
      961 aaactggttg actcggaatt gagcttccgc tgaatagatt caactatagt ctgccaaccg
     1021 tgatctagat aaagtacacc ttgattggcc agtatcacct gagcaaaggc ggctccggca
     1081 gacaattcct gagggctgtt ggcatatgtg gaaagcctga caaaggtttc aattgtctgg
     1141 cgcacccgag ttctcttcac gttcaacttc agccattgcg caaggttgac tgacataatc
     1201 ttgtcgagat ctaaatgcgg cagcctgcgg aaaaatcgtg ccagctctat tttctcggac
     1261 caattcaggt aggttgccct gaatagactc gagagattga ccggcagttc atacacttca
     1321 cctgcgaata tggccaggtt ttcccggttc aggggcggtg cggcgccgcg gtagtcgatg
     1381 ccgttatttt gcaggtagcg atgggccaat ccacctgcat acagagcgtg gggaccgaaa
     1441 tttaggtagt atccctcttt gtgagtcgtc tgcgctctcc caccggcttt actgctcttc
     1501 tcaaatacaa ccaccttcgc cccgccctta ctcagctcca gtgccgctac catgccggcc
     1561 acaccggcgc cgataatcgc tatcttcttt tgcctggtgt ccataacttt tacctcgcca
     1621 catcgtggat attttctatc cacgattatg acaccaagac ttgccagcaa ccataaattt
     1681 tagataaaat atatccacga tgaaaatgag tcagggaatc gaatggtcaa ttcactgctg
     1741 cgccttgttg gcgctgctgc cgcccaagcg caccctgcgc atcgaaaaaa ttgcccagtt
     1801 ctatgacctg ccggtgccat acctgagaaa gcacttccag gatttatccc gggcaggcat
     1861 cctcaaaacc acttccggac ccaaaggcgg ctatcgcctg gccagagaag ccaaagacgt
     1921 gaatctgctc cacatatacg aagcaatcga gggtcccgag cacgcttttc gctgcaacga
     1981 aattcgccaa aatggtccca acacctgcgg tcagaaggtc tacccggctg cctgcaatat
     2041 agccacttct atgtggaagg cggaagaggc cttccgccgg gaactggaaa aggtttcgct
     2101 ggaagacatc gtgaaaacgg cagccctgca gataagcccg gagcgtcggc agaaatcggc
     2161 acaatggctg gaagaagcgc tatcgaatca gtaaccggca caacactgat tgagcccttc
     2221 gcaagagaag cgcctatgcg tattgttgaa ctaaggtcat tttctcacga caaactcgat
     2281 tgttgcgctt cagaggcgcc ctgcttgaga attttggcat actcaaggga aggatcgagg
     2341 gctatcgcct tgttacactc ttccaccgcc tctgggtatt gcttgcgcga tgaatagacg
     2401 acactgcgaa tcaaataaat gtatggctcc ctttcgtcta ttgccatcgc agcctcggca
     2461 tcatcccagg cgcggtcgaa ttcgtccaac tggcaataga gaattcctcg cttcttatac
     2521 gactctacgc tcttgccgtc agcactaatc tccagcgagc atttgtctac atgtagccgc
     2581 atttgcgcct ctacatcgct gacagtgact ttctgggccg ccataagtgg cgcgcagacc
     2641 tttcgccagg gcaaaccgag agcacaagcc tgtttcaaga ccacttgcaa cattgagcat
     2701 cgcacgcagt gtttggtctc tccaattgga ggcttgccgc aaaattcaca ttgagccatt
     2761 tagcattcct ttcgcattat ttcaaagtct tcaggctccc agagcagcat cgccatcaag
     2821 ccgctgccgt ttaaaagcat ctttcccacg aacaagcact tcgaagctct tgcttgggct
     2881 caaagtgata tcggtcatcg caagca
//
LOCUS       DAOQNZ010000395         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18338_length_2905_cov_3.973333, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000395 DAOQNZ010000000
VERSION     DAOQNZ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18338_length_2905_cov_3.973333"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKW73_08120"
     CDS             complement(<1..683)
                     /locus_tag="PKW73_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73289.1"
                     /translation="MNEFGSLLFLPSIAAILSLVIQNLAVTRKFCAIAAWIQLVIVLS
                     MLSQILTGHVPNLPFVSGLAVDRLGACFIVLTTFVVASCLTHADYFFQAAANRGDEYS
                     ANRVKVFYASTNIFLMAMTTVFCCDSLGYLWIAIEATTLSSAPLVYFERNKYALEATW
                     KYLIVCSVGIAFALLGTVFIFAASQRGDIQAATLNVSELIAGAKALNFPLLRLGFIFC
                     LLGYGTKAG"
     gene            complement(704..1354)
                     /locus_tag="PKW73_08125"
     CDS             complement(704..1354)
                     /locus_tag="PKW73_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73290.1"
                     /translation="MTLDTFEALSVLSAILGILILGTTLLRTNLSFYRMHTLAIAAVT
                     AWIAHITKEDHLFVVALGVFLIKVIFIPNFLNWVMKKVDVGSDKQAFIAAPLSMHLGA
                     LMLGLSFLLSKQIPGLVGGHDSHLGSTAAMSILFTGVLLMLTRRAALSQIVGFLVIEN
                     GIYLFAITQTHGMPMLVEMGLLLEVLVGVMISGLLLFNIKKSFEHIDVSQLTELKD"
     gene            complement(1375..2337)
                     /locus_tag="PKW73_08130"
     CDS             complement(1375..2337)
                     /locus_tag="PKW73_08130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNA73291.1"
                     /translation="MESLLTDLNRQEWLRAGAFFLSLIFMPPLTVGIIRKTKARFQNR
                     IGAPIFQPFYDLAKLLKKDETISNHVSWIFRSAPSINLSLLFIVACLAPWTTLHPFGL
                     PGDLFLLIYLLAFSRFFTVLSALDAGSAFGGFGASREVTLALLVEPAIMLSLASLGVT
                     AGTSDLTTVFSFENVKLQSQTGLWFIAGCGLFLSSLVELSRMPADDPTTHLELTMVHE
                     AMILENSATNLALAELTHFVKIVVLLGISSQCFLHSLPWVWHTSEITRALASIGGVFF
                     MAAFIAVIESVAVKLRWTKLPEFIAYSVAMSIFCVLIALSRSTN"
     gene            complement(2337..>2905)
                     /locus_tag="PKW73_08135"
     CDS             complement(2337..>2905)
                     /locus_tag="PKW73_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73292.1"
                     /translation="LAAAQILLSCLCILLGTSAGISLSAFSPALTLLTQCSNDTGSFF
                     PLAIGQLSLVGVFLTAAIYLIILGPKTKDIKTYITWDCGYGPLSARAEETGSSFSHPI
                     GRIFSQILQLNVETEIQGKDRRHFPEAIRVETKMSPFIETKAYRRFLDVVNWLSLTLT
                     RLQTGSIHVHLLYLFLTMIVLVLLGTTL"
BASE COUNT          737 a          646 c          924 g          598 t
ORIGIN      
        1 ataccggctt tggtgccgta gcccagcagg cagaagatga agcccaggcg cagcaggggg
       61 aagttaagcg cttttgctcc ggctattagc tccgatacat tcaaagtagc ggcctgaatg
      121 tcgccccttt gactggctgc gaaaatgaag accgtgccga ggagggcgaa ggcaataccg
      181 accgagcata caatcaggta tttccaggtg gcctcgaggg cgtacttgtt gcgctcgaag
      241 tagactagcg gggctgatga aagtgtggtg gcttcaatag cgatccagag gtagccgagg
      301 ctgtcgcagc agaaaacggt ggtcatggcc atgaggaaga tgttggtgct ggcgtagaaa
      361 actttgacgc gattggcgct gtattcatcg ccccgattgg ctgccgcctg gaagaagtaa
      421 tcggcatggg tgaggcaact ggctactaca aaggtagtca gcacgatgaa acaggctccc
      481 agcctgtcca cggcaagacc gcttacgaag ggcaaattgg gaacgtggcc ggtgagaatt
      541 tgggagagca tggaaaggac gatgacgagc tgaatccagg cggcgatggc gcagaacttt
      601 ctggttacgg ccagattctg aatcaccaga ctgagtatgg ctgctattga cggtaagaat
      661 aagagggagc cgaattcgtt cataacttct tccttgaatc ccgtcagtct ttcagttcgg
      721 taagttggga aacgtcgatg tgttcgaagc tcttcttgat gttgaagagc aaaagcccgg
      781 atatcatcac gcccaccagt acttccagca aaagccccat ctccaccaac atgggcatgc
      841 cgtgggtctg ggtgatggca aaaagataga tgccgttttc aattacgagg aagcccacaa
      901 tctggctcaa ggcggcgcgt ctggtcagca ttaaaagtac gccggtaaag aggatggaca
      961 tggcggcggt ggagcccaag tgagagtcat gtcctcctac gagcccaggg atttgtttgc
     1021 tcaagagaaa ggagagcccc agcatcaatg cgcccagatg catggaaagc ggtgcggcaa
     1081 tgaaggcctg cttgtcgctg ccgacatcaa cctttttcat aacccagttg agaaagttgg
     1141 gaatgaaaat gactttgatc aggaaaactc ccagtgccac cacaaagaga tggtcttctt
     1201 ttgtgatgtg ggcgatccag gcggtgacgg cagcaatcgc cagggtgtgc atgcgataga
     1261 aggacagatt ggtgcgcaaa agggtggtgc cgaggatgag aatacccaga atggctgaga
     1321 gaacagagag tgcttcgaag gtgtccaggg tcattggtta ttctccggtt tgcgtcagtt
     1381 ggtgctgcga gaaagtgcaa ttaagacgca gaagatactc atggccactg agtaggcgat
     1441 gaattcaggt agcttggtcc agcgcagttt gacggctacg ctttctatca cggcgatgaa
     1501 ggcggccatg aaaaagacgc cgccgatgga ggcaagggct cttgtgattt cggaagtgtg
     1561 ccagacccag ggtagggaat gcaggaagca ctggctggag atgcccagta atactacgat
     1621 tttgacgaag tgcgtcagtt ctgccagggc caggttggtg gccgagtttt ccaggatcat
     1681 ggcttcatgc accatggtca attccaggtg ggtggtggga tcgtcggcgg gcatgcgcga
     1741 gagctccacc aggcttgaga ggaaaagacc gcagccggcg atgaaccaga ggcccgtttg
     1801 agactgcaat ttcacatttt caaaggagaa gaccgtggtc agatcgctgg taccggcggt
     1861 gacgccaagg gaggccaggc tgagcatgat ggccggctct accaacaggg ccagggtgac
     1921 ttcgcggctg gcgccaaaac cgccgaaggc cgagccggcg tcgagggctg agagcactgt
     1981 aaaaaaccgg ctgaaggcta agagataaat gagaaggaag aggtcgccgg gaagcccgaa
     2041 gggatgcagc gtggtccagg gagccaggca ggccacgatg aagagtaggg atagattgat
     2101 agacggggca ctgcgaaaga tccaactgac atgattgctt atggtttcgt ctttcttgag
     2161 taatttggcc aggtcgtaga agggctgaaa aatgggtgcg ccgatgcggt tttggaaacg
     2221 ggccttggtc tttcgaatta tgcccacggt caggggcggc ataaagataa gcgagaggaa
     2281 gaaggcaccg gctcgcagcc attcttgtct atttagatca gtgagaaggc tttccattac
     2341 aaggtagtcc ccaacaaaac cagaacaatc atggtcaaaa agaggtagag caagtgtacg
     2401 tgtatgctgc ctgtctgcaa gcgggtgagg gtcagggaaa gccagttgac cacatccaga
     2461 aaacgcctgt aggctttggt ttcgatgaat ggtgacattt ttgtttccac tcgaatggct
     2521 tccgggaagt gccggcggtc tttgccttga atttccgttt caacattgag ctgcagaatt
     2581 tgagagaaga ttctgccgat gggatgagag aagctggagc cggtttcctc ggcccgggcg
     2641 gataggggac cgtagccgca gtcccaggtg atataggttt ttatgtcttt ggttttggga
     2701 ccgaggataa tcagataaat cgccgctgtg aggaagacgc cgaccaggct gagctgacca
     2761 atggccaggg gaaagaagct accggtgtca ttactgcact gggtcaagag ggtgagggcg
     2821 gggctgaagg cgctcaagct gataccggcg gaggtgccca gcaagatgca cagacaggaa
     2881 agtaagattt gcgccgccgc caaat
//
LOCUS       DAOQNZ010000396         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18342_length_2905_cov_3.023509, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000396 DAOQNZ010000000
VERSION     DAOQNZ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18342_length_2905_cov_3.023509"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..2520)
                     /locus_tag="PKW73_08140"
     CDS             complement(160..2520)
                     /locus_tag="PKW73_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNA73293.1"
                     /translation="MAKRRSQARKKNQGALGRISGPLKKITIFGVVVGCLGLGYLGAS
                     FWKQLQDIPDVTMVERYEPIEAIQIFDGQDHLICTVEGDEDRRVVPLNQISNHMQQAI
                     LAAEDHHFYEHNGINFFSIIRAFLANMQAGHVVEGGSTITQQLAKNLFFSDAGRTVDR
                     KLKEAYVAWDLERRYSKERILNMYLNQVYFGNNAYGIERAASRYFDKTATRLNIAESA
                     FLAGLVKAPSELGTVKGRDQAIARQREIIAKMVEYGYITDKQAKDAKAQKLAFKKGIN
                     PLQKYPYYISYVLEVLRERFSQAEMRRQGLRVYTNLDTTAQELAEQNLNEGIKKAPKG
                     VTQGALVSVRVNDGAIIALVGGVGDFWKNQFNRATNPHTVGSSFKPFVYLTAFSKNAY
                     NPDSIIDDSPLVIHQPWGLPDYAPKNFDRKFYGRITVRRALALSRNVPAVKIGKEVGM
                     ESVIETARLAGLTAKLDPNLSLALGSSAASPLEMAGAYSTFARFGVTIKPQVIRKIEN
                     NRGQVIEVFEPKVDRAFQVEPIARLVDCMQDVVRWGTGTQAKLADRPVAGKTGTADEG
                     KDIWFIGFTPDLVTAVWGGNDENHSIEGKNVTGGVVMAKIWHNYNEAYYKAHPTPPGN
                     FMVPSPISPDESKPAGSGEQLARSSDTAKEDTATAGTSETGSTTGSTDGSSTAAETGS
                     STDTTAQTPGTAPTPGSDKTPPTAPPVTTPAETPPTAPTPGGGNEGPQGKLPQGGDRT
                     LSEKLPEPAPARTAAKPVQSKNAEGVVMRLYPYNPGPQYLPAEKTR"
     gene            complement(2747..>2905)
                     /locus_tag="PKW73_08145"
     CDS             complement(2747..>2905)
                     /locus_tag="PKW73_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA73294.1"
                     /translation="RHTFATHLLEGGADLRVVQDLLGHTTINTTQIYTHVSLERLRRV
                     YMNTHPRA"
BASE COUNT          533 a          723 c          885 g          764 t
ORIGIN      
        1 gctccgacgg cttcggaatc accgctcaaa aagaaatttg tattgcggcg cccattgcct
       61 cttgtttgga caaagagcac aagcaacaca cttactacca cggccagcca gatcgctggt
      121 gcattgttcc ttctggcaat tagcataggg cgcccgacct tatctagtct tttcggccgg
      181 cagatactgc gggccgggat tgtaaggata aagtctcatg accacacctt cggcattttt
      241 actttgcacc ggtttggcgg cggttctagc tggagccggt tccggcaatt tttcgcttag
      301 ggttctgtcg ccgccctggg gcaacttgcc ctgtggtcct tcgttgccgc ctcccggtgt
      361 gggagctgtg ggcggtgtct cagccggggt ggtaacggga ggagcggtgg gcggtgtctt
      421 atcggaacct ggtgtggggg ccgtgcctgg tgtttgtgct gtggtatcgg tgctgctacc
      481 ggtctcggcc gccgtgctgc tgccgtcagt actgccggtg gtgctgcctg tttcgcttgt
      541 gccggcggta gcggtatctt cttttgcagt gtcgctggag cgtgccagtt gttcaccgct
      601 accggctggt ttggattcat cgggacttat tggtgagggc accatgaaat tgccgggtgg
      661 agtggggtgg gctttatagt aagcctcgtt gtagttgtgc cagatcttgg ccatcacaac
      721 gccgccggtg acgttcttgc cctcgatgga gtgattttcg tcgttaccgc cccagacggc
      781 tgtgaccagg tccggggtga agccgatgaa ccagatgtct ttgccttcat cggcggtgcc
      841 ggttttgccg gccacgggac ggtcggccag cttggcctgg gtgccggtac cccatctgac
      901 tacatcttgc atgcagtcta ccaggcgggc aattggttca acctggaagg ctcggtccac
      961 cttgggctcg aagacttcaa tcacctgacc gcggttgttt tcgattttgc gaatcacctg
     1021 cggtttgatg gtgacgccga atctagcgaa agtggagtag gcaccggcca tttccagggg
     1081 tgaggcggcg gaactgccca gggcaagaga aaggttagga tcaagcttgg cggtaagacc
     1141 ggccagtctg gctgtttcaa tgacgctctc catgcccact tccttaccga ttttgacggc
     1201 cggtacgttg cgtgacagag ccagggcccg gcgcactgtt atgcgtccgt aaaatttgcg
     1261 gtcaaagttt ttgggtgcat agtcgggcaa accccagggc tggtggatga ccaggggcga
     1321 gtcgtcgatg atgctgtcag ggttgtaggc gtttttggaa aaggccgtca ggtagacgaa
     1381 gggcttgaag gaggagccga cagtgtgggg attggtggcc cggttgaact ggttcttcca
     1441 gaaatcgcct acgccgccca ccagggcaat gatcgctccg tcgtttactc ttacggaaac
     1501 gagagcgccc tgggtgaccc ccttcggtgc tttcttgatg ccttcattga gattttgttc
     1561 ggccagttcc tgggcggtgg tatcaaggtt ggtgtagacc ctcagaccct ggcggcgcat
     1621 ctctgcttgt gagaagcgtt cccgcaaaac ctccagcaca taacttatat agtacgggta
     1681 cttttgcagg gggttgatgc cctttttgaa agccaatttt tgcgctttgg catctttggc
     1741 ctgtttgtcg gtgatgtagc cgtattccac cattttggca atgatttctc gctgtctggc
     1801 aatagcctgg tcgcggcctt tgactgtgcc cagttcgctt ggtgccttga ccagaccggc
     1861 tagaaaggct gattcggcga tgttcaagcg ggtagctgtc ttatcgaagt agcgggaagc
     1921 ggcccgttcg ataccgtagg cattgttgcc gaagtagacc tggttcaaat acatattgag
     1981 gatgcgctct ttgctatagc ggcgctccag gtcccaggct acgtaagctt ccttcagttt
     2041 gcgatcgacg gtacgaccgg cgtcggagaa gaagaggttt ttggccaact gctgagtgat
     2101 ggtggaaccg ccttccacca cgtgtccggc ttgcatgttg gccaggaagg cacgaatgat
     2161 actgaagaaa ttgataccgt tatgttcgta gaagtggtga tcttcagcgg ccaggatggc
     2221 ctgctgcatg tggttggaaa tttgattgag aggcaccacg cgcctgtctt cgtcaccctc
     2281 gacagtacaa atcaggtggt cttgcccgtc gaagatctga atggcttcaa tgggttcata
     2341 gcgctcgacc atggtcacat caggaatatc ctgcaattgt ttccagaagg aagcacccag
     2401 gtagcccagt ccgagacagc ccaccaccac accaaaaatg gtgattttct tcaagggccc
     2461 ggaaatgcga ccgagcgcgc cctgattttt ctttctggcc tgtgatctgc gttttgccac
     2521 ttttgcctga gccggtcgtt ttctatatcc gtgagagttt tcgaagatgt cagttagtac
     2581 gtttcatcgg cattataact tagttgtgca ggtgtgcctg ttagcgtcgc ttcatggtaa
     2641 aagccgctgt taagggtggg taagaattgt gcctgacaca ggcgcgatcc aagcaaaaaa
     2701 gaggggtttc cccctctttt gagtgttgct taatgtttgc gttatatcaa gcgcgcggat
     2761 gggtgttcat atagaccctt ctcagtcttt ccaggctgac atgggtgtat atctgggtgg
     2821 tgttgatggt cgtgtgaccg agcaggtctt gaactacgcg caggtcggcg ccgccctcca
     2881 ggaggtgggt ggcgaaggtg tgtct
//
LOCUS       DAOQNZ010000397         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18426_length_2898_cov_2.931059, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000397 DAOQNZ010000000
VERSION     DAOQNZ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18426_length_2898_cov_2.931059"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /locus_tag="PKW73_08150"
     CDS             complement(<1..1647)
                     /locus_tag="PKW73_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNA73295.1"
                     /translation="MTNLGRVLPLSLSLQLAFTGSGFSPLLAQAPPPSPLPAGKPVSP
                     PLPPALPKSSDSPRRITRPAQSQPAAALPTRIPRRAHTTDSRPDSTLKPQVPSVDVIP
                     NTVPSSGQAQAPAPAPAPVAPPAYQTSQAGNAVEQYPTIGRLEEVTLGRSQPGAPIGE
                     RLSTLETVVFKRNFNDDSLFDRTERLKKTILGGMEAERADLNGNNAPPVYNNNDLENL
                     PTLETVREVNYLDELALRPENLQPSTPEQVGQFAVEIINTERRNYGLGALSPDTLAEK
                     LAREQAGYQGRRSLISHFNEAGLGPDRRYTLEEGSDALTECIASVKTAEVGASKVCKA
                     GAVKLLKLLLSRQDDRDAILSPDATHIGFAICENAGGDRFIGTCEILNRHSLMGPLPS
                     QAALGEKVEVKGVFHSPYVFDRVTLAFEALASPNSSYGAADEGEEALPYFPPLDYVAY
                     SNRSERDYSKAVTAIKIGGMVAAVAGGMFFPPIALAAPLIAVAGSPGEPRPVSDIPVK
                     GGVKIDGAAFSVKVPLNNENKEGLYYITVWGTLGKGQKSIP"
     gene            complement(1772..2656)
                     /gene="pgeF"
                     /locus_tag="PKW73_08155"
     CDS             complement(1772..2656)
                     /gene="pgeF"
                     /locus_tag="PKW73_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNA73296.1"
                     /translation="MEAGSDNDKNQSFQERLQRSLSFSEWQVKELDGLKLTTSPLLSR
                     QEGLVHAFTTRHGGSCPSPFDNFNLGRSVGDEKVKESALKNRQRLCRALKVDHDRLHV
                     PGQVHSGHVVKVDGSMKPDLAGVDGLATASKNLPVLLHFADCVPVMIYEPELRLLSIV
                     HAGWRGTAQSIAVMGVERLKELGGDPRRMLAAVGPAIGTCCYPTGLDVVEKLMATVNP
                     SGSPAVDKLARKGLVEERQGQPRPNLKAINALQLLNQGLAQVDVSEFCTSCRPELFYS
                     HRQSGGITGRQGAVGCLV"
     gene            complement(2702..>2898)
                     /locus_tag="PKW73_08160"
     CDS             complement(2702..>2898)
                     /locus_tag="PKW73_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA73297.1"
                     /translation="EDPHWQALNGLPADAGMELTRLSVPQERFLEPEEVASLVLYLAS
                     PMARGITGQAINICGGLSLH"
BASE COUNT          653 a          819 c          817 g          609 t
ORIGIN      
        1 aggaatagac ttctgcccct tcccaagtgt tccccagacc gtaatgtaat agagcccttc
       61 tttgttttca ttgttgaggg gcaccttaac ggagaaagcg gctccatcga ttttgactcc
      121 ccctttgaca ggaatgtccg aaaccggtct cggttcacca ggagagccgg ccaccgcgat
      181 caaaggcgcc gccagggcaa tgggcggaaa gaacatgccg ccggccactg ccgccaccat
      241 accgcctatt ttgatagcgg tcaccgcctt actgtagtca cgctcggaac gattggaata
      301 ggccacataa tcgaggggtg ggaagtatgg cagggcctct tctccttcat cggcggcacc
      361 ataagagcta ttgggagagg ccagggcctc gaaggccagg gttacccggt caaagacata
      421 gggactgtga aagactccct ttacctctac tttttcacca agggccgcct gactgggcag
      481 aggccccatg agcgaatgtc gattcaaaat ttcacaggtg ccgataaagc ggtccccgcc
      541 cgcattttca cagatagcaa aaccaatatg ggtagcatcc ggtgaaagga tggcatcacg
      601 atcgtcctgc ctggagagaa gcagtttgag gagtttgacg gcaccggctt tacagacctt
      661 gctcgcacca acctcggcgg tcttgacaga agcgatgcat tcggtgaggg catcggagcc
      721 ttcctcgagg gtatagcggc gatcaggacc caaaccggct tcgttgaaat gagaaatgag
      781 cgagcgccgc ccctggtagc cggcttgctc tcgtgccagc ttctcggcca gggtatcggg
      841 cgaaagggca cccagaccat agttgcgtcg ttcggtgtta attatctcta cggcaaactg
      901 accgacttgc tccggggtag agggctgtaa gttctcaggt ctgagggcca gctcgtccag
      961 ataatttact tccctgacgg tttccagggt gggcagattt tccagatcat tattgttgta
     1021 gacgggcggc gcattattgc cgttcaaatc ggctcgctcc gcttccatac cgccaagaat
     1081 tgtctttttg agcctctcgg tacgatcgaa aagagaatcg tcgttgaagt tacgcttgaa
     1141 gaccactgtt tccagggtgg aaagccgctc tccaatggga gcgccaggct gactgcgccc
     1201 cagggtcact tcttccagac ggccgatggt ggggtactgc tcaacggcat tgcctgcctg
     1261 ggaagtttga taggcaggag gcgccaccgg cgccggagcc ggtgcaggag cctgagcttg
     1321 accgctggat ggcaccgtat tcggtattac atccacggaa ggcacctggg gcttgagcgt
     1381 cgaatccggc ctgctgtcgg tggtatgggc ccggcgtggg attcgcgtcg gtaaggcggc
     1441 ggcaggttgg ctctgagcag ggcgcgtgat gcggcgaggc gagtcggaac tcttgggtag
     1501 agccggtggt aagggcggtg aaaccggctt accggccggc agcggactgg gaggcggcgc
     1561 ctgggccagg agcggagaaa agccggagcc cgtgaaagcc aattgcaaac tgagggaaag
     1621 aggcaagact cttcccaaat tcgtcaaaac ggctctcatt cctgccactg cccaccccga
     1681 gtgcattaga tatattttgc acaatcccgg caatattaca aatcgtcccc agaaattaga
     1741 agagataaat tttaaatgcc cggctgcctc cctagaccag gcaaccgacg gcgccctgcc
     1801 ggccggtgat gcccccggat tgacgatggg aatagaaaag ctcgggacgg caacttgtgc
     1861 aaaattccga gacatccacc tgggccagtc cctgattaag caactgcagg gcattgatag
     1921 ccttcaggtt gggtcttggt tgaccctgcc tttcttccac caggcccttt ctggccaact
     1981 tatccacggc cggcgaaccg ctcggattga cagtagccat gagcttttct actacatcta
     2041 aaccggtcgg ataacaacaa gtaccaatgg ccggaccgac cgccgccagc atgcggcggg
     2101 gatcgccgcc cagctccttc agcctttcca cgcccatcac ggcaatagac tgggccgtgc
     2161 cccgccaacc ggcgtgcacg atggaaagca gtcttaattc aggctcgtaa atcatgaccg
     2221 gcacacagtc tgcaaaatgc aagagaacag gcaaattctt gctggcagtg gccagaccat
     2281 caactccggc caggtcaggt ttcatgctgc cgtccacttt caccacatga cccgagtgga
     2341 cttgccccgg tacatgcaaa cggtcgtggt caaccttaag ggcccggcac aacctctgac
     2401 gattcttaag agcgctttct ttgacctttt catcacccac ggaacgaccg agattgaaat
     2461 tatcaaacgg gctggggcag ctaccgccgt gccgggtagt gaaagcatgc accagtcctt
     2521 cctgccgaga cagcaagggc gatgtggtga gctttaagcc gtccagttcc tttacctgcc
     2581 attcactgaa ggaaagtgac cgctgtaacc gctcctggaa agactgattc ttgtcgttgt
     2641 cgctgcctgc ttccatgtcc gccttctata tattggatat acgtctactc aaacgggagg
     2701 gctaatgcaa ggaaagtccc ccacatatat ttatagcctg tcctgtgatt ccgcgcgcca
     2761 ttggcgaagc aagatagagc accagggaag ccacttcctc cggctcaagg aaacgctcct
     2821 ggggcacgga aagtctggtc agttccatac cggcatcagc aggcaagcca ttgagagcct
     2881 gccagtgcgg atcttcta
//
LOCUS       DAOQNZ010000398         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18441_length_2896_cov_5.412531, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000398 DAOQNZ010000000
VERSION     DAOQNZ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18441_length_2896_cov_5.412531"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PKW73_08165"
     CDS             complement(<1..690)
                     /locus_tag="PKW73_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA73298.1"
                     /translation="MPRQISTQHTKNGAHPGTINQGTRAAEPNQTRISLIMSQNNNNN
                     NGPVPGPNSPTNTKPDDSNLTEEILKKGGKGKDEVEKMGKIDRADEVVEESFDAKFRT
                     ANSPVHRAVWDKTPIELFRANDVTGAAPTYFQAMDQCVAIVVRHRLAGTLFGDDQKVT
                     ATVMQELAEAGYWGMLIDKKYGGQGVTITHFMQFLTRMAAEGDPTVAGMASIHGCIGA
                     VDPMCAYGTEAQ"
     gene            complement(1140..>2896)
                     /locus_tag="PKW73_08170"
     CDS             complement(1140..>2896)
                     /locus_tag="PKW73_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73299.1"
                     /translation="IRKRALSQAEQPVYFPYADVLSVGLLYNKTETNRHLTYRDLDEL
                     ALDTDTAIDAAVSNLEAFSKERFRLVQSGFYTSPWKDNFDGARLLLTDLFKELEVEGD
                     IVALTPAADALFVTGSRDADGLKLLTVLSAAVMRGANPLAALPLVLRNNLWQPFLPEP
                     ENEAFFEIYRYRLTVLQHHYEQQSRLLADTDHLAFSNDRQGGTSANFIYTPPFLIEED
                     QRTGSCYSKTSVLEGSPANIPTAEVVEFYRKDRSGEKQCVARAPFHLLQEELGNKLKP
                     DNYFKPERWFVSEFPDSQELSALGTMAAGKPPGRWFQAPANQLSELSRRFGINIPDTA
                     ELADSLEVNPDSIVQVFRLPGTVSQLQERQYAKGMCAILCPELAPEKQLLRLEKVDAC
                     SSRTVFFARSKHQGMAVLKLLKRENYDTMAIRNAFIKQSRELSALEERFGLSLPEKTS
                     IFGSLQQNQNYLRQDFRLTAAWPDLLDFYQAQLMGDHLQVLVPGPKAASNLWDASLRN
                     LQSRFSRQGQAERQDNQAEIFLINESRGLAVSVTLAEPDPAMLSTFEDVRANLSTQAR
                     RPQELSFSLTRKILPVQP"
BASE COUNT          576 a          724 c          877 g          719 t
ORIGIN      
        1 ctgggcctcg gtgccgtaag cgcacatggg gtcgacggcg ccgatgcagc catggatgga
       61 agccatgccc gccacggtgg ggtcgccctc agccgccatg cgagtgagga actgcatgaa
      121 gtgggtgatg gtgacgccct gaccgccgta cttcttgtcg atgagcatgc cccagtagcc
      181 ggcctcggcc agctcctgca tgacggtggc ggtgaccttc tggtcgtccc cgaagagggt
      241 gccggccagg cggtggcgca cgacgatggc cacgcactga tccatggcct ggaagtaggt
      301 aggggcagcg cctgtgacat cgttggctcg gaaaagctcg atcggggtct tgtcccagac
      361 ggcgcggtgt acggggctgt tggcggtgcg gaacttggcg tcgaagctct cctcgaccac
      421 ctcgtcggcg cggtcgatct tgcccatctt ctccacctca tccttgccct tgccgccctt
      481 cttgaggatc tcctcagtca ggttggaatc gtcgggcttg gtgttggtgg gggaattcgg
      541 accagggaca gggccattat tgttgttatt gttctggctc atgattagcg aaatccttgt
      601 ttggtttggc tcagccgctc ttgttccctg gttgatggtg ccagggtgag cgccgttttt
      661 ggtgtgctgc gtagagattt ggcgaggcaa aatctcactc gcggcagaaa gaagatcggt
      721 tatcaacccg acctgataag agagcgccac cgtggcgaac tcttgtgttt cccggcccgg
      781 caagtgtcgt cttcaaccgt gttcctcaac agcattcttt cgatacggaa gtctgtgcga
      841 ctacggcaag gctgagctgg tcttgcggca gtaacggcag tgaaagggct tgtgtttctt
      901 ggaggttgaa gaagtcaggt taaagggatg ggaaatatga acgagtctaa cgactcgtaa
      961 aggactgttt caactgcggg agaatgagat gtatgccaca ttgtttgatt ttgagtactt
     1021 gtcaagaaag cgcggctggc gcaatcacta aaacggttaa gagattttgg ttgtcatgat
     1081 gaagcggcgc tcagggctga accggttaag agattttgtt tgtcatggtg gggtggcgtt
     1141 cagggctgaa ccggaagaat tttcctggtc agggagaaac tcagctcctg gggtcttctt
     1201 gcctgggtgc tcagatttgc tcttacatct tcaaaggtgc tgagcatagc cgggtcaggt
     1261 tcggcaaggg tgacagacac agccagccct cgcgattcat taataaggaa aatttccgcc
     1321 tgattgtctt gtcgttctgc ttgcccctgc cgggagaatc ggctttgcag attcctgagg
     1381 gaagcgtccc acagattaga tgctgccttg ggccccggta ccagcacttg caagtggtcg
     1441 cccatcagct gggcctggta aaagtcgagc aaatcgggcc aggctgccgt gaggcggaag
     1501 tcttgccgga ggtagttctg gttctgttga agactaccga aaatgctggt cttctcaggc
     1561 aatgacagac cgaagcgctc ctctagagcg gaaagctctc tgctttgttt gatgaaggca
     1621 tttctgattg ccatggtgtc atagttctcg cgtttgagca gtttcaaaac agccattccc
     1681 tggtgtttgc tgcgggcgaa aaacactgtc ctgctggagc aggcgtccac tttctccagt
     1741 cgcagtaact gtttctctgg tgccagttcg gggcagagta tggcacacat gccttttgca
     1801 tattgccttt cctgaagttg agagaccgtg ccaggcaggc gaaaaacctg cacaatgctg
     1861 tccggattga cttccaggct gtcagccagt tctgccgtat cgggaatatt gataccgaag
     1921 cgccgggata actcggacaa ctgatttgcc ggtgcctgaa accacctccc tggtggtttt
     1981 cctgctgcca tggtacccag ggctgagagt tcctgggaat ccggaaattc gctcacaaac
     2041 cagcgttccg gcttgaagta gttgtccggc ttgagcttgt tgcctagctc ttcttgcaga
     2101 aggtggaatg gtgctctggc tacgcattgt ttttcgccgg agcggtcttt gcgatagaat
     2161 tccaccactt cagcggtggg aatgttggcg ggcgagcctt ccagcacact ggttttgctg
     2221 tagcagctcc cggttctttg atcttcttcg attaagaagg gcggtgtgta gatgaaattg
     2281 gcagaggtgc cgccctgccg gtcatttgag aaggccaggt ggtcggtatc ggccaggagt
     2341 ctgctctgtt gttcatagtg atgttgcaga acggtcaaac gatagcgata aatctcaaag
     2401 aaggcttcat tctcaggctc gggcaggaag ggctgccaga gattgtttct caggaccagg
     2461 ggcagggcgg ctagaggatt ggctcccctc ataacggctg cgctcagcac ggtgagcagc
     2521 ttcagaccgt ctgcgtcccg agatccggtg acaaagagcg catccgcggc cggggtcagg
     2581 gctacgatgt cgccctctac ctcaagttct ttgaaaagat ccgtcaataa aagacgcgca
     2641 ccatcgaagt tgtctttcca gggggaagta tagaagccgc tctgcaccag gcgaaacctc
     2701 tctttgctaa aggcttccag gttgcttacg gccgcatcga tggcggtgtc cgtgtccagt
     2761 gccagctcgt ccagatctcg gtaagttaag tgtctgtttg tctcggtctt gttatagaga
     2821 agtcctaccg aaagcacatc agcgtaggga aagtagaccg gttgctcagc ctggctcagt
     2881 gccctttttc gtataa
//
LOCUS       DAOQNZ010000399         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18445_length_2896_cov_3.541711, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000399 DAOQNZ010000000
VERSION     DAOQNZ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18445_length_2896_cov_3.541711"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PKW73_08175"
     CDS             <1..434
                     /locus_tag="PKW73_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73300.1"
                     /translation="LSLAASICKGDSWNLIHMVESLPFSFKSFDEVRHQLEIDDLEQF
                     GSLVKRILSAPESFNCQSLNIRLTGGDFASGVASVNESELSGTAAKILQLGEALGFRA
                     YLSCQDNGAYTRKGYGRCEGVWDYPRDLLPEYEEWIQLVIR"
     gene            892..1779
                     /locus_tag="PKW73_08180"
     CDS             892..1779
                     /locus_tag="PKW73_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNA73301.1"
                     /translation="MNTSLDKLLAVLLFILGLLFLLSGNSSGGTTANLTPCRLSSVEE
                     VKNCLLTNRRDATDFSSLLQGESVLIFGETYLSVDNRFELMTALPALKNLGFTRLALE
                     AMPSSRQSLVNEYKQGRVPRQEIKAAIKSIWGHSPESYMQVIDAALDQGIEVVFLDRD
                     YERVDLHADNWQELEKQARDRREKHWLNVLTRQLEQHPGARMLVLVGSDHISPQAATM
                     PLQCRLRQSGIAAASIAIAGGGVFYDDVLTRAARQGRLERSKFFVRLAPIKGAWQGMD
                     FYLHLPQTSEKRKIFSYSE"
     gene            1822..2514
                     /locus_tag="PKW73_08185"
     CDS             1822..2514
                     /locus_tag="PKW73_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73302.1"
                     /translation="MNANVEPEVSAISAEQSTALPESEFLEAPPTATESKPTFSAELL
                     EPAEADNGRGTLVVVDMQAPFVSACKDPLLVASVVEQVELAVSRGWAVVLVEVKPWAY
                     GATLAPILDLLKDKYEHYSACQKEGDDGSAPVLETCERLHFSEQFFRVTGVLIGACVL
                     STARGLVHRRQDCLVRVMKEACGTSLEDTALAWSNFPQGERLVVSSKAIDGAGSTDVV
                     EPDSQAESLMET"
BASE COUNT          640 a          701 c          782 g          773 t
ORIGIN      
        1 cacttagtct tgccgcttcc atttgcaaag gtgattcttg gaaccttatt catatggtcg
       61 aaagccttcc tttttcgttc aaaagtttcg atgaagtcag gcatcagttg gaaatcgatg
      121 acttggagca gttcggttct ctggttaagc ggattctctc cgcgcctgaa tctttcaact
      181 gccagtcctt gaacatcagg ttgactggtg gtgatttcgc ctctggtgtg gcttcggtca
      241 atgaaagtga actatcggga actgccgcga agatcctcca gcttggtgaa gctcttggtt
      301 tcagagccta cttgagctgt caggacaacg gcgcttacac ccgcaaaggc tacggtcgct
      361 gtgaaggagt ctgggactat ccgcgagact tgttgcctga atatgaggaa tggatacagt
      421 tggtaattcg ctagcataca gggtgttata cgtggaccat ggatggtccg tcctcagtta
      481 tttccgcatt ttaaaaatgt gcggatattc actaaggcat ataagaggag gttgtagttg
      541 gttagggagc gctgtgtgca aaattgccgg gccactatca actggcccag ttcggcagat
      601 ttttttgtag ttgaggctgc cgggcatggt ggtttgatga cggttcacca ttagtggtgc
      661 catagaaagc ctttgacaag cctttgaccg ggcttgagcg cagctgttta ccgctgcaat
      721 tgctttgtga ataccgcttg gtttcaggtt gccgctcgtc tgactcttgc tccttgcttc
      781 atggctctgt tagaaacaat tgcttattca atacttttct caaaggtgtt taaaccctta
      841 ccaatttttc ttccaaatct cccgaaaacc tgaaactagc ctggagtctc gatgaatacg
      901 agtctagata agctattagc tgtcctcctg ttcattttag ggctgctctt tttgctgagc
      961 ggcaattctt ccggcggtac caccgccaac cttacaccct gccgcctttc gagtgtggaa
     1021 gaggtgaaaa actgcttgct gactaacagg cgggatgcaa ctgatttttc cagtttgctg
     1081 caaggagagt ccgttctcat cttcggagag acgtatctca gtgtggacaa tcgttttgaa
     1141 ctgatgaccg ctttgccggc tctcaagaac cttggtttta ccaggcttgc tcttgaagca
     1201 atgccttctt cccggcaatc cctggtaaat gaatacaagc agggcagagt gccccgccaa
     1261 gaaataaagg cggctattaa gtcgatctgg ggacacagtc ccgaatcgta catgcaagtt
     1321 attgatgcgg ccctggatca gggtattgaa gtggtcttct tagatcgcga ctacgagcgt
     1381 gtcgacctcc atgccgacaa ctggcaggaa cttgaaaaac aagcccggga taggcgggag
     1441 aaacactggc ttaatgtgct taccaggcaa ttagagcagc atcctggcgc tcgcatgctt
     1501 gtactcgtag gcagtgacca tatttctccc caggcggcca ccatgccctt gcaatgccgc
     1561 ttgcggcagt ccggcatcgc tgccgcctcc attgctattg ccggtggtgg cgttttctat
     1621 gacgacgttc tcacccgagc tgcccgccag ggaaggcttg aaaggagcaa attctttgtg
     1681 cgtcttgccc cgatcaaagg tgcctggcaa ggtatggatt tctatctgca tctgcctcaa
     1741 accagcgaaa agaggaagat tttttcttat tccgaataag cactttcaaa tagagaggag
     1801 acataaaaat gtctatccgt tatgaatgcg aatgttgagc cggaagtttc ggcaatatcg
     1861 gcagagcagt ctactgcgct gccggaatca gagtttttgg aggcgccccc gaccgccacc
     1921 gagagtaagc cgaccttcag tgccgagcta ttggagccgg cggaagctga taacggtcgt
     1981 ggcactctgg tggtagtaga tatgcaagcg ccctttgtca gtgcctgcaa agatccgctc
     2041 ctggttgcct ctgtggtaga gcaggtggag ctggcagtat ctcgcggctg ggccgttgtt
     2101 ctggtggaag tgaagccctg ggcttatggt gccaccctgg cgccgattct agacctgttg
     2161 aaagacaagt acgagcacta cagtgcttgc cagaaggaag gtgatgatgg ctcggcgccg
     2221 gtgcttgaga cttgtgaaag acttcacttc agtgaacaat tctttcgcgt cacaggggtt
     2281 ttaattgggg cttgtgttct ctccactgct cgtggtctgg tgcatcgtcg gcaggactgt
     2341 ctggtgcggg ttatgaagga agcctgcggc accagtctgg aagacacggc cctggcctgg
     2401 agcaactttc ctcaagggga gcggcttgtg gtcagcagca aagccattga tggtgctgga
     2461 agcactgatg ttgtcgaacc tgattcccag gctgagtctt tgatggaaac ctagagcgga
     2521 agcggaaatt tctttgcaag ggagcgctct ttgcctgact ttgcctatat gcaggcatgt
     2581 aacgaaaccg caaggatgta aagtgatgga attcacatgt tcttgcaggc tgttggtctg
     2641 gaacccggct ggaagccttg gttccagccg ctaacgcaca gccctgaagg cttttaacca
     2701 tctcatcctg agcagttcgg atatttatgg ggcagtctag cgaaccctcg cgaggaagcc
     2761 ccttgtaaac atgctgtttg cagcctgtac ctggaattgc cgctgggata gggtgagatg
     2821 tcttcttcct tccccaaatt caaccgagtt tcaatctctt ttaccacctg atcaagctgc
     2881 tatcgcacat gcaccg
//
LOCUS       DAOQNZ010000400         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18524_length_2889_cov_3.369442, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000400 DAOQNZ010000000
VERSION     DAOQNZ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18524_length_2889_cov_3.369442"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..647
                     /locus_tag="PKW73_08190"
     CDS             177..647
                     /locus_tag="PKW73_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73303.1"
                     /translation="MESDGNKTCFITNLKCAWNKLLEKFFRKGGESQSPAESRELSPV
                     LHEGLKETGRAMSQSISRLSQNRLHAMEGDRSNQLGKLRAAARHEVEVDGKDAWFGKE
                     VVGLAAEGPVRARANVQARAQARMGSRVTEARLTNTRLRALAEALEPPEEKHDE"
     gene            complement(661..1263)
                     /locus_tag="PKW73_08195"
     CDS             complement(661..1263)
                     /locus_tag="PKW73_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNA73304.1"
                     /translation="MMTGNYLESIARLLQEYLQHWSEDQPRLEVLLKQVEEEDRELTN
                     RKNMVGHLTGSALVVDDQNRVLLIKHNMLKRWLQPGGHLDFEEWPADGSLRELVEETG
                     ISEDGHGLKLHRLHGKFGEGLPIDVDSHKIPENPGKGEGPHLHHDFQYVYELSEGAHK
                     LNLQEEEVSQFRWLPLEELLSGAYGVRLQRVAGKILTFSR"
     gene            complement(1269..1826)
                     /locus_tag="PKW73_08200"
     CDS             complement(1269..1826)
                     /locus_tag="PKW73_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73305.1"
                     /translation="MTSVEDKQKNFATIVDGKVVDDCERVAVCIKGSVLGFPATIEAI
                     KPDFPFGTSFFLETDVLHEHQGTKPSFTIVVTPKVAKGLSGFLGRLLLIDAHERPTGI
                     KVFDSELLAHSEDLDICQRFIGYPGMAEKLRDLQRFNGFREFHIQGGKGLCLICPTNF
                     KGLSYDVARETLQVMATIAQVLFEF"
     gene            complement(1833..>2889)
                     /locus_tag="PKW73_08205"
     CDS             complement(1833..>2889)
                     /locus_tag="PKW73_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73306.1"
                     /translation="RLDMAPAQNIKTLNQYKRKNALSLTALNVLRLLTLCLVLAQALP
                     AQAEGGDEKPAKKGYNREAVARYNQGREFYKTGYFNKAVEAYKQAIALDDRMEQAYCN
                     LGLTYIQQKNYAKAQEALLKAVALKPDRASSLNGLASVLFFRGKKEEAIEKWQEAVKV
                     DPKFASAYLNMGKAMESLDQLDDAQKAFSTALKLQPDLAQAYYSLGLLMLKRNHDGQA
                     HYLLSRAVELEPESEYARDARKQVAALTDKFAKEAKASGETEEEPMKVVGANIDHELK
                     NHTKAHALSEPNVKVTHDSQKAEKPAKRGFNLLKRKDGKEGKEKAEAVKMKLYHPSGD
                     KNQTDLKARPPVEPGFE"
BASE COUNT          589 a          748 c          805 g          747 t
ORIGIN      
        1 acaattgttt cgtctttgca actattgaaa ggcagatctt agccccgtcg tcgtcgttgt
       61 ctgagagtgt aacaaaaagc caatttaccg gactctatcc cccctctgga gggatgtcag
      121 gggttataac ggtataaggg gctcccgtag ttgccgcgtt ttctttggtg aatcagatgg
      181 aatcagacgg aaataagact tgctttatca ctaatctcaa atgcgcctgg aataagttac
      241 tggagaagtt cttcagaaaa ggaggggagt ctcaaagccc cgctgagagc cgagaattga
      301 gcccagtctt acacgaaggt ctcaaggaaa caggtcgggc tatgtctcag agcatcagtc
      361 gcctttctca aaatcgactg catgccatgg aaggcgatcg ttcaaaccag ttaggtaagc
      421 tgcgggctgc cgcccgccat gaagtggaag tggacggcaa ggacgcctgg ttcggtaaag
      481 aggtggtggg actggccgcc gagggaccgg tgcgggccag agccaatgtg caggcccggg
      541 cccaggcacg catgggctct cgggtgacgg aagcgcggct gaccaatact cgtttgcgag
      601 ccctggccga agctcttgag cccccggaag aaaaacacga cgagtagacc aaacgaaaaa
      661 ttagcggctg aaggttagaa tcttgccggc cactcgttgc agtctgactc cataggctcc
      721 gctaagcaac tcctctaaag gcagccagcg aaactggctc acttcctctt cttgcaggtt
      781 gagtttgtgg gcgccttctg aaagttcgta tacgtactga aagtcgtggt gaaggtgcgg
      841 tccctcgcct ttgcccgggt tctcaggaat tttatgggaa tcaacgtcga tgggcagtcc
      901 ctcaccgaat ttgccgtgta ggcggtgcaa cttcagcccg tgcccgtcct cgctgatgcc
      961 ggtttcttcc accagttctc gcaaggagcc gtccgcgggc cattcctcaa aatccagatg
     1021 accaccgggt tgcagccagc gcttcaacat attgtgtttg atcaggagga cgcgattttg
     1081 atcatccacc accagcgcgc tgccggtgag atgaccgacc atgtttttgc gattggtcag
     1141 ttcgcgatct tcttcttcca cttgcttgag cagtacttca agacgcggtt ggtcttccga
     1201 ccagtgctgc aaatactctt gcaaaaggcg ggcaattgat tctaggtagt ttcccgtcat
     1261 catggttctc aaaactcaaa caagacctgg gctattgttg ccatcacctg tagtgtctct
     1321 ctcgccacgt cgtaactgag acctttgaag ttggtcggac aaatgagaca aagtcccttg
     1381 ccgccctgaa tgtggaattc gcgaaagccg ttaaaacgct gcaagtctct cagtttctcg
     1441 gccatacccg gatagccgat gaagcgctgg caaatgtcca ggtcttccga atgggccagt
     1501 agttcgctgt caaagacttt tatgccagtg ggcctttcgt gagcgtcaat gagcaagagc
     1561 cgccccagga agccggataa gcctttcgct actttgggtg tcaccacaat agtgaagctg
     1621 ggttttgtgc cttgatgctc gtgcagcaca tcggtttcca gaaagaagct ggtgccgaag
     1681 ggaaaatcgg gttttatcgc ttcgattgtg gcgggaaagc ccagcacgga acctttgatg
     1741 cagacggcga cgcgctcgca gtcgtccacc actttcccat ccacgatggt ggcgaagttc
     1801 ttttgtttgt cttctacgct tgtcactgtt ttctattcga aaccgggctc gaccgggggt
     1861 ctggctttca agtcggtctg attcttatcg cccgagggat gatagagttt catttttacc
     1921 gcctcggctt tttctttgcc ttccttgcca tccttacgct tcaataagtt gaagccgcgc
     1981 ttggctggtt tttcagcctt ttgtgagtca tgggtgactt tcacattggg ctcggagagg
     2041 gcatgggctt tggtatggtt cttcagttcg tggtcgatat tggctcccac cactttcatg
     2101 ggctcctctt cggtttcgcc cgaggctttc gcctctttgg caaacttgtc ggtgagggcg
     2161 gccacttgct tgcgagcgtc tctggcgtac tcggattccg gctccaactc tacggcgcgg
     2221 gaaagcaggt agtgggcctg tccatcgtgg ttgcgcttga gcatcaacaa gcccagggaa
     2281 taataagcct gggccaggtc cggttgtagt ttgagcgccg tggaaaaggc cttctgggca
     2341 tcatcgagct ggtccaggct ttccatggct ttgcccatgt tgagataggc ggaggcgaat
     2401 ttggggtcta ccttgacggc ttcctgccac ttttcgatag cttcttcctt cttgccccgg
     2461 aagaagagca ccgaggccag accattgagg gaggaggccc ggtccggctt gagggcaaca
     2521 gccttgagca gcgcttcttg agcttttgcg taatttttct gttgaatata ggtgagaccg
     2581 aggttgcagt aggcctgttc catacggtca tcgagggcaa ttgcctgttt ataggcttcc
     2641 acggctttat tgaagtaacc ggtcttgtag aactcccgcc cctggttgta gcgggccacg
     2701 gcttcccggt tgtagccttt cttagctggt ttttcgtcac cgccctcagc ctgcgccggc
     2761 aacgcctgcg ctagaaccag gcatagggtt agaagccgga gaacattgag cgccgtgagg
     2821 ctcagtgcat tttttctttt gtactggttc aaggttttaa tgttctgcgc cggagccata
     2881 tctaatcgt
//
LOCUS       DAOQNZ010000401         2888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18533_length_2888_cov_3.546770, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000401 DAOQNZ010000000
VERSION     DAOQNZ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18533_length_2888_cov_3.546770"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PKW73_08210"
     CDS             <1..655
                     /locus_tag="PKW73_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNA73307.1"
                     /translation="LFGLIGSMVVLVPQACGAAEPKFKEPPPPDPSRFVISGRIVDRR
                     GEAVKDCTVRLWEPKGKVSLETRCKPSGEFSIPHNESGALTLEVLPPEASGYAQSLVE
                     KLPGNESRKLIVRLRPGHLVTGRILDCDGKRGLKGLIIKVEPLDKGEDGQAHLHGSGG
                     CETLKKGVFSFRLTEGKKKMLIINELYSSVPPVSAKEFQVSGDTHLGAIVLSAAHKD"
     gene            675..1991
                     /gene="larC"
                     /locus_tag="PKW73_08215"
     CDS             675..1991
                     /gene="larC"
                     /locus_tag="PKW73_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNA73308.1"
                     /translation="MKIAYFDCSFGAAGDMLLGAFLDAGFSLAVLQEELSKLHLPQGE
                     FQLALSKVHRTSLLASYLQVQIEPVSHEEASNHEHEHVGQPHDHHHHHEHEHHEHRHT
                     HAGGERHLSEILSLIENSTLAAPVKSTATRVFQRLGQAESKVHGVPVEDIHFHEVGAT
                     DAIVDIVGFAVAFHGLGIEKAYVSALPLGSGTVKTSHGIFPVPGPATLNLLVDAGAPT
                     REMNVGYECLTPTGAAILTTVADAFAVVPAFSRISACGYGAGTLNPASHPNVVRIMLG
                     ESGINANESTAGNSCNSEIVACLETNIDDCSPQVLAFAAEALLTKGALDVSLTACTMK
                     KGRPGHLLTVLSRPEEAQALAEIIMKETTALGVRSFLCERLTLDRDFVSVNVMGEKIK
                     VKVARDKMGKLINVQPEFDDCKRLSLASGMALKEVIAECLRLCALS"
     gene            complement(2049..>2888)
                     /locus_tag="PKW73_08220"
     CDS             complement(2049..>2888)
                     /locus_tag="PKW73_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73309.1"
                     /translation="PERVVENQIKNPEHFVFRLDFEAKDRGRDTFLVLKSSTLNKFAD
                     GLIEETLKLSNLRVVFVRMDTNADVFFDKVQELDTPELLRKLFRVTSIEQINRILHAW
                     CDKRAESSIASAYVESDELVVQACDLKHYRVKFAEFVGLAELPTRERERFQIDEFGNH
                     VFWPSQNVSIDLDVVRYKADDQFRHAKDMDALSDYKDLLGKAILEVMSKHKLTQTTLK
                     QRGGPAERHLYRIGRSAQELTPTMIDRLSKAHGLSSDKYVEELIMACDEIAEMEAQNI
                     CAE"
BASE COUNT          654 a          755 c          756 g          723 t
ORIGIN      
        1 cttgttcggt ttaattggca gtatggtggt gctagtgcct caagcttgcg gggcggccga
       61 gcccaagttc aaagagccgc cgccaccaga tcccagccgg tttgtcatat ccggtcgcat
      121 tgtcgatcgc cggggcgagg ccgtcaaaga ctgcacggtg cggctctggg agccgaaagg
      181 caaggtttct ctggagactc gctgtaaacc gagcggtgag ttttccatac ctcataatga
      241 atcgggtgcc ttgaccctgg aagtgcttcc tccggaagct tcaggctatg ctcaatcgct
      301 ggtggaaaag ctgccgggca atgaaagtcg caagcttatc gtgcggctgc gccccggtca
      361 tctggtcacg ggacggattc tcgattgcga cggcaagcgt ggcttgaagg gcttgatcat
      421 aaaagtggaa ccgctcgata agggcgagga cggtcaggct cacctgcatg gcagcggggg
      481 ctgcgagacc ctcaagaaag gagtattctc tttcagattg acggaaggga agaagaaaat
      541 gctgatcatc aacgagctct attcttccgt gcctcccgtc agcgccaagg agtttcaggt
      601 gtcgggcgac acccatcttg gtgccatcgt cctcagtgcc gcccataaag actgaaattt
      661 gcgcgggagt tagcatgaaa attgcctatt tcgactgtag tttcggcgcg gccggagaca
      721 tgctgctcgg cgcttttctg gatgccggtt tcagcctggc ggttttgcag gaggaactct
      781 ccaaactgca cttgccccag ggggagtttc aactggcact ctccaaggtg catcgcacaa
      841 gccttttggc atcttacctg caagtgcaaa tagaacctgt cagtcatgaa gaagcttcga
      901 atcatgaaca tgaacatgtc ggtcagcccc atgaccacca tcatcaccat gagcatgaac
      961 atcatgagca caggcatacc catgccgggg gtgagcggca cttaagtgag attctttctc
     1021 taatagaaaa ttcgactttg gccgcgccgg tcaaaagtac cgccacccgc gtctttcagc
     1081 gcctggggca ggcggaaagc aaagtgcacg gagtgccggt ggaagatatt cactttcatg
     1141 aagtcggtgc caccgacgcc attgtggaca tcgtcggttt tgccgttgcc tttcacgggc
     1201 tcggtatcga gaaagcctat gtctcagccc tgcccctagg cagcggcacg gtcaagacca
     1261 gtcatggaat ttttccggtg cccggccccg ctaccctcaa cctgcttgtt gatgccggtg
     1321 cccccacaag ggagatgaac gtcggctatg aatgtcttac accaaccggt gcagcgatac
     1381 taaccacggt ggcggatgct tttgccgtgg tgccggcctt ttcccgcatc agtgcttgtg
     1441 gctacggggc cggtaccttg aatcctgcca gccatccaaa tgtggtacgc atcatgcttg
     1501 gtgaaagcgg cattaatgca aatgaaagta ccgccggcaa ttcttgcaat tccgagattg
     1561 tggcctgcct ggagaccaat atcgacgatt gttcgcccca ggtcttggcc tttgctgccg
     1621 aggctcttct tactaaaggt gctctcgatg tgagcctcac ggcctgcacc atgaaaaagg
     1681 gaagaccggg gcatttactt acggtcttaa gccgaccgga agaagcccag gcactggcag
     1741 agataatcat gaaagaaacc accgctctgg gtgtacgttc ctttctttgc gagaggctta
     1801 ccctcgatag agattttgtt tcagtaaacg ttatgggcga gaaaatcaaa gtgaaggtag
     1861 cccgcgacaa aatgggcaag ctcatcaacg tgcaacccga gttcgatgac tgtaaacgcc
     1921 tttcgctcgc gagcggcatg gccctgaaag aagtcatcgc tgaatgtttg aggctctgcg
     1981 cgctttcgta gaggagttgg acacggcaag agtgaccgcc agaggttcta gcgcttgtgc
     2041 catgcgtttc attcagcgca tatgttttgg gcttccattt cggcaatctc gtcgcaggcc
     2101 ataatcagtt cttcaacgta cttatctgaa gacagtccat gcgcttttga cagtcgatct
     2161 atcatagtgg gtgtaagttc ttgcgcgctg cgcccgattc gatatagatg acgctctgcc
     2221 ggaccgcctc tttgtttgag agttgtctgc gtgagtttat gctttgacat aacctccagt
     2281 atggccttcc caagtaaatc tttgtagtct gacaaagcat ccatgtcttt tgcatgtcta
     2341 aactggtcgt cagccttata gcggacaacg tctaaatcta tcgacacgtt ttggctaggc
     2401 caaaatacat ggttcccaaa ttcatcgatt tgaaaccgtt ctcgttcacg tgtcggcaat
     2461 tcagccagtc ctacaaactc agcgaatttg actcggtaat gcttcaagtc acaagcttgc
     2521 acgacaagct catcgctctc tacgtaggcg cttgcaatac tgctttcagc gcgcttgtca
     2581 caccatgcgt gtaagattct gttgatttgc tcgatggatg ttactctaaa tagctttcga
     2641 agcaactccg gcgtatccag ttcctgaact ttgtcgaaga acacatcagc attcgtatcc
     2701 atgcgtacga agaccactct gagattactc aatttcaggg tttcttctat aagtccgtct
     2761 gcgaacttat tcaaggtgct cgatttgaga actagaaatg tatcccgccc tctgtccttt
     2821 gcttcgaaat caaggcgaaa gacgaaatgc tctggatttt tgatttgatt ttccacgacc
     2881 ctttccgg
//
LOCUS       DAOQNZ010000402         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18573_length_2885_cov_3.019788, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000402 DAOQNZ010000000
VERSION     DAOQNZ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18573_length_2885_cov_3.019788"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKW73_08225"
     CDS             complement(<1..453)
                     /locus_tag="PKW73_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73310.1"
                     /translation="MAAKVELFPFISFMIPYIEAITPSSLFITACVIWSSGAVLTVVS
                     DLTKLRLEDRWMTGLSIAGSLATIVGSIKSYTQTQAFGFSGFPNFGLAPLTCLTDSLS
                     VPFTLLLGLVSLAAALATPSFPHTLATKSQHNHFFRLTFLMLSGILMVL"
     gene            624..2330
                     /locus_tag="PKW73_08230"
     CDS             624..2330
                     /locus_tag="PKW73_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNA73311.1"
                     /translation="MPARKTWESIKATITGQFQALTHPLSLPDAPQRTVPYDGLRGLV
                     QNFRYDSLAGFIIFLIAMPLSLGIAMASGLPPVAGLISAAVGGILVSQISGSHVTING
                     PAAGLIVVVVGAVDALGGGNEGYRNTLAAIVVSGLTLFALGRLKCGELGKFFPASVVH
                     GMLASIGLIIILKQLPVMLGVQLKVREPFEIVMHLPHVFTHLNPYVALIGIVSLLILI
                     VHPKLPLAALKKIPAPIMVIAAGILMSVLFQMNESHDYTFFKQVFSVNPGKLLVQVPT
                     DFSKLLTFPDFGALRMPRFWPAVFSITLVQGIETVLSCAAVDKLDPFKRKSNLSKDLS
                     AVGFGSAVSGAIGGLPMIAEIVRSTANIASGARTRYANFFHGVFLLVCVATCAALLNR
                     IPLAALAALLVFTGYRLASPRHFKEMHEIGAEQSLIFVSTILITLATDLLIGVFSGVA
                     IKLFLHVLRGVKPAELFRGAVDVQEGGENDIYISPRGACVFSNYLTLKHALDSLQPGR
                     NVTIQFDKTTFLDHTVMEHLDDFRTDYEHLGGKVGFIGLDRLKCISEHPQCSRRLERQ
                     RL"
     gene            2439..>2885
                     /locus_tag="PKW73_08235"
     CDS             2439..>2885
                     /locus_tag="PKW73_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA73312.1"
                     /translation="MAKILVVEDETDLVGLVKGWLEREHHLVETACDGLEALIRMETN
                     KYDVVLLDMMLPSLDGLEILKRYRRGGGAAGVIMITARSHVDDKELGLDAGADDYLTK
                     PFQLKELAARVRAVLRRNHVPTSDVLRFRDLVIDPHEFKVLKGGKDV"
BASE COUNT          594 a          699 c          817 g          775 t
ORIGIN      
        1 aagtaccatc aagatgccgg agagcatcaa aaaggtgagg cggaagaagt gattgtgttg
       61 tgacttggtg gccagagtat gggggaaact gggggtggcg agagcagccg ccaggctcac
      121 cagtccaagc agtaaagtga aaggcacact gaggctgtca gtcaggcagg tgagcggtgc
      181 cagtccgaaa ttgggaaagc cgctgaagcc gaaggcctgg gtctgcgtat agctctttat
      241 tgacccgact atggtagcca gactgcccgc tatgctcaaa cctgtcatcc agcgatcttc
      301 cagtctcagt ttggtcaggt ccgagactac ggtgagcacc gcaccagagc tccagatgac
      361 gcaggctgtg atgaagaggc ttgagggcgt gatggcttct atataaggga tcataaatga
      421 aataaagggg aataattcga ccttagcagc catttgtgat cggactgtga tcaaccgcca
      481 aacttttgta aacccaccac tgtccggtac tgctggggat gactgactcc ctctcgggtt
      541 tttatggtga tcacaagttg atcatatttg cttgctaggg ttgctttata aagtcgtact
      601 atcagcataa atggtcgcta tctatgcccg ctcgtaagac ctgggagtca atcaaggcta
      661 ccattacagg tcaattccaa gctctcacgc acccgctctc ccttcccgat gcgcctcagc
      721 gcacggttcc ctatgatggg ttgcgagggc tggtgcagaa ttttcgctat gacagtctgg
      781 ccggtttcat catatttctc attgccatgc ctctttctct tggcattgcc atggcttccg
      841 gattgccgcc ggtggccggt ctgatcagtg cggcggtggg cggcattctg gtgtcgcaga
      901 tcagtggttc tcacgtcacc atcaatggtc cggcggccgg cttgatcgtt gtcgttgtgg
      961 gggccgtaga tgccctgggc ggcggcaacg aaggttatcg caataccctt gccgccattg
     1021 tggtgtcggg gctcactctt tttgctctcg ggcgtttgaa atgcggagaa ctgggcaaat
     1081 ttttcccggc ttcggtggtg cacggcatgc ttgcttccat cgggctgatt atcatcctca
     1141 agcaattgcc ggtgatgctg ggggtgcaat taaaggtgcg cgagcctttt gagattgtca
     1201 tgcatttgcc ccatgttttc acacatttaa atccatatgt tgctttgata ggcattgtca
     1261 gtttactcat tttgatagtg catccaaaat tgcctctggc ggccttgaag aagatacccg
     1321 caccaattat ggtgatagca gcgggtattc tcatgtcggt gctctttcaa atgaatgaga
     1381 gccacgacta cactttcttc aagcaggtgt tctctgtcaa tccgggcaaa ctgctggtac
     1441 aggtgcccac cgactttagt aagctcctta ctttccctga tttcggggcg ttgcgcatgc
     1501 cgcgattctg gccggccgtc ttcagtatta cgctcgtgca gggcattgaa actgttctct
     1561 cttgcgcggc agtggataag ctggatccgt tcaagcgtaa gtctaatctg agcaaagacc
     1621 tgtctgccgt cggattcggc agtgctgttt ccggtgccat cggcgggctg cccatgatcg
     1681 ccgaaattgt gcgaagcacg gctaatattg ccagtggtgc ccgaactaga tacgccaatt
     1741 tctttcacgg cgtcttcctg ctcgtctgtg tggctacctg tgccgccctc ctcaatcgta
     1801 ttccgctggc ggcattggcg gctcttctag tgtttacggg ctatagattg gcctctccca
     1861 ggcacttcaa ggagatgcac gagataggcg ccgagcagag cctgattttt gtcagtacta
     1921 ttttgataac tttagctacc gacctgctaa tcggtgtctt tagcggtgtg gccatcaaac
     1981 tcttcctgca tgttttgcga ggcgtcaaac ctgccgagtt gtttcggggc gcggtggatg
     2041 tacaggaggg cggtgagaat gatatctaca ttagcccccg cggcgcttgc gtgttttcca
     2101 attatctcac tctcaagcat gccctggata gtttgcagcc cggtcgaaac gtcaccattc
     2161 agtttgataa gactactttt ttggaccaca cggtcatgga gcacctggat gactttcgta
     2221 ccgactatga gcatctgggc ggcaaggttg gtttcatcgg gctggatcgg ctcaagtgta
     2281 tatccgagca tccgcagtgc agcaggcgtc tggagaggca gcgtctttag gcacagtcga
     2341 gaattgactc ttgcatatat gttcttgtat gcgatggagc ggcattttgc ctggtccggc
     2401 ggcagctatt ttccggtctg aagagttaga attaccctat ggctaagatt ctcgtagttg
     2461 aagacgaaac agacctggtc ggtcttgtaa agggctggct ggaaagagag catcatctgg
     2521 tggagaccgc ttgcgatggt cttgaggccc tgattcgcat ggaaacgaat aagtatgatg
     2581 tggtcttgct tgatatgatg ttgccgtctt tggacggcct tgagatcttg aagcgatatc
     2641 gccgtggcgg cggcgctgca ggcgtaataa tgattaccgc acgctcccat gtggatgata
     2701 aggagcttgg tctggatgcc ggtgcggatg actatttgac caagcctttt cagctaaagg
     2761 aattggctgc cagggtgcgg gccgtgctgc ggcgcaatca tgtgccgact tcggatgttt
     2821 tgcgctttcg tgatctggtt attgatcccc atgaattcaa ggtgctcaaa ggcggtaaag
     2881 atgta
//
LOCUS       DAOQNZ010000403         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18600_length_2883_cov_3.532178, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000403 DAOQNZ010000000
VERSION     DAOQNZ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18600_length_2883_cov_3.532178"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKW73_08240"
     CDS             <1..332
                     /locus_tag="PKW73_08240"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNA73313.1"
                     /translation="GGETSLDDMIASVGADQEAILLTRVWYVREVDPATKLLTGMTRD
                     GTFLVRDGKIARPIKNLRFNVSLFDLLNNIVALGESVGAAGEEGIPAVVPPMLVKDFN
                     FTEVTKF"
     gene            544..1422
                     /locus_tag="PKW73_08245"
     CDS             544..1422
                     /locus_tag="PKW73_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNA73314.1"
                     /translation="MKFHPKGVEVVLGAGGAKGPGHTGVLKAIEELKIPVSKVTGVSV
                     GSLVAALYANGLSPEQIFQELVQGLKNRKDPTLLLQALTPADPISLAVGGPIDLVPAM
                     REMVARLNLEARDELKIVACDILRHEPVVFEGSQYDLAKALTASCALPTVLRPVWHQD
                     GMTPRLLVDGAVYHYNPTDFCQDTAIVSSFKPATEFPQEFETVFDLYFHMREMYAPIA
                     GHRRYVDPHKHVVLEIGLADVAGLNFGISVEKARAMEEDGYQTAMKMLKEAIAAGRIH
                     TEESLAAALSASATSA"
     gene            1538..2635
                     /locus_tag="PKW73_08250"
     CDS             1538..2635
                     /locus_tag="PKW73_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73315.1"
                     /translation="MLARIKKAAGSLAYLAMLYLSWKWFVGQLDYSFNLSCVILSGSW
                     LALTAHGLSENFRTYFDILSRLKVRVPMIFGLSLSALVLFTPWDPAATVRHFLTPDGL
                     SSHLLSPLPLVAAIELFFWLLVYGAYRRNASRFKKQGHGPLPKGAWVNPPREALQEGD
                     LILTSGRIAKRLRESVGHGEVVVDLRKEELYTLTSYMERGAMTQPLSVMTDQKLEFGH
                     YIALRLRKPLTAEQRALIPGLVGIMLEQNRRYIEAAKARRLAVYKWLHLPAFITGLLD
                     KTVPVSGYDWWGLFTGRRAQDRWTCVGACLELYHRIGVETKTYGTGLFGLGTGVLDPI
                     MPTRFLADPAFRLLSESDRKAFAAGNSKAVN"
BASE COUNT          594 a          920 c          783 g          586 t
ORIGIN      
        1 aaggcggcga aaccagcctt gacgacatga tcgcctccgt cggtgccgac caggaagcaa
       61 ttctgctaac aagggtctgg tatgtgcggg aggtggatcc agccactaaa ctactgacag
      121 gcatgacgcg ggacggcacc ttcctggtgc gggacggtaa aatcgccagg ccgatcaaga
      181 acctgcgctt caacgtcagc ctcttcgacc tgctcaacaa catcgttgct ctcggtgaaa
      241 gcgtcggcgc cgccggcgaa gaaggtatac cggcagtggt tccccccatg ctggtaaaag
      301 atttcaattt cacggaagtg acaaagttct agattgcaac aagcctgttt tctgaaagcc
      361 gatgcgtccg ccagccgggg cgtcgttttc cagaagtcag gccaatagcc ggctgcaaga
      421 gtcggctgcc ataggccggt acgctttcga gccggtcact tcacggtcag gtgcctccac
      481 tggtggtacc tgctcgtctt cattccactt ctctctcaac ccctctattt gacggaggtt
      541 accatgaaat ttcaccccaa gggggtcgaa gtcgttcttg gtgccggtgg cgccaaggga
      601 cccggtcata ccggcgtcct caaggccatc gaagagctga agatcccggt gagcaaagtc
      661 accggcgtct ccgtgggttc tctggtggcg gctctttacg ccaacggact cagccccgag
      721 cagatctttc aggaactcgt gcaaggtctc aagaaccgca aagatcccac tctccttctc
      781 caggcactca ctccggctga ccctatcagc ctggcagtgg gtggccccat cgacctcgtt
      841 ccggccatgc gcgagatggt ggcacgcctc aacctcgagg cccgcgacga actcaagatc
      901 gtcgcctgcg acatcctgcg acacgagccg gtagttttcg agggcagcca gtacgacctg
      961 gccaaggccc tgaccgcttc ctgcgccctg cctacggttc tgcgcccggt ctggcaccag
     1021 gacggcatga caccgcgcct gcttgtggac ggcgccgtct atcactacaa cccgacggac
     1081 ttctgccagg acacggccat cgtcagctcc ttcaagccgg ccaccgaatt cccgcaggaa
     1141 ttcgaaacgg tcttcgatct ctatttccac atgcgtgaaa tgtacgctcc cattgccggt
     1201 catcgccgct atgtggaccc gcacaagcac gtggttctgg agatcggact ggccgacgta
     1261 gccggtctca acttcggcat cagcgtcgaa aaggcgcgtg ccatggaaga ggacggctac
     1321 caaacggcca tgaagatgct caaggaggcc atcgccgccg gccgcatcca cacggaagag
     1381 agcctcgctg ctgctctttc ggcaagcgcc acaagcgcct gacacacccc tcccaactgc
     1441 aaaagcggct ggcggaagca aaccataaca agggaaaaga gcgcctcgca atcggcgctc
     1501 ttttccacat aaaccacatg tctatttcga ggttcctatg ctggcaagaa taaagaaagc
     1561 ggctggctct ctggcttacc tggccatgct ctacctgagc tggaaatggt tcgtcggtca
     1621 gctcgactac agtttcaatc tctcctgtgt aatactctcc gggtcctggc ttgctctcac
     1681 ggcccacggc ctgagcgaga acttccgtac ctacttcgac attctctccc gcctgaaagt
     1741 gcgggtgccc atgattttcg ggctctccct ctccgccctg gtgcttttca cgccctggga
     1801 tccggctgcc acggtcagac atttcctgac cccggacggg ctttcctccc acctgctctc
     1861 gccacttccc ctggtggcag caattgaact cttcttctgg cttctggtgt acggcgccta
     1921 ccgccgcaat gccagccgct tcaagaaaca gggacacggc ccgctgccga aaggggcctg
     1981 ggtcaatccg ccccgagaag cactgcagga aggagacctg attctgacca gcggccgcat
     2041 cgccaagcgc ctgcgtgaaa gcgtagggca tggggaagtg gtggtggatc tgagaaagga
     2101 agagttgtac accctcacct cctacatgga gcggggtgcc atgactcaac ctctttcagt
     2161 gatgaccgac cagaaactgg aattcggaca ctacatcgcc ctgcgcctca ggaaaccgct
     2221 taccgccgag caaagagcgc tcattcccgg gctggtgggc atcatgctgg agcagaatcg
     2281 gcgctacatt gaagcggcca aggccaggcg cctggctgtc tacaaatggc tccatctgcc
     2341 ggctttcatc accggcttgc tggacaagac cgtaccggtt tcgggctacg actggtgggg
     2401 actctttacc ggtcgccgcg cccaggaccg ctggacctgc gtgggtgctt gcctggaact
     2461 ctatcaccgg atcggtgttg agaccaaaac ttacggcacc gggctcttcg gtctcggcac
     2521 cggcgtgctc gatcccatca tgcccactcg ctttctggct gatccggcct ttcggctttt
     2581 gagcgaaagc gatcgcaaag cctttgccgc aggcaattca aaggcagtaa actagctgtc
     2641 tcaggctgcc aggcagggct gaacaggcac aaatttctca agggggaacc aactcaaaag
     2701 gctggttccc cctttttccc agcccctcct atcttctata gtttaattcg gtctgcagcc
     2761 ttgttgcggc cagcgccagg gctgatgcag acagatctat cagggtgatg cagcacagcg
     2821 gccaacctcc ttgaaaaact tgcagcgggc tctgcctcca gccaaccagc ccaacctagc
     2881 tca
//
LOCUS       DAOQNZ010000404         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18657_length_2877_cov_4.790220, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000404 DAOQNZ010000000
VERSION     DAOQNZ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18657_length_2877_cov_4.790220"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKW73_08255"
     CDS             complement(<1..329)
                     /locus_tag="PKW73_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73316.1"
                     /translation="MHAGNLHDGLDQKICLECNRQFTGVVKACPHDGAALVALARDPM
                     IGTCLMGKYDIIDVIGHGGMGVVYKGRQVLMERTVAIKMLQSQHIADSQSVQRFLREG
                     KASCRMN"
     gene            complement(478..1671)
                     /gene="recF"
                     /locus_tag="PKW73_08260"
     CDS             complement(478..1671)
                     /gene="recF"
                     /locus_tag="PKW73_08260"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNA73317.1"
                     /translation="MFVSRLTVKNFRNYQDASLSLHPVRNLLIGDNAQGKTNLLEAIE
                     LLCNIKSGRATSDRDLILAGAESTLIEITYVCQGIEETVSLILSLKPRLSRVLKVNGM
                     KVSRSELGGLSRLTAVSFSTRDLALVRGGPKERRDWLDQVLVKLRPGFAEVVSQYEKI
                     VQQRNKLLRLCFERGQAPDEEEIKVWDIQLARVGARLVKARAAVLEKLLPQAEANLTR
                     VSGLKERLSAVYLFRVKQRDVEEDGEEDANLRLAELLAMKEEDLAKRIYLNLKDRRAE
                     EVVRKQTLTGPHRDDIVFAINDFAAHAFASQGQQRSLVLALKLAELKLVEEALSEPPL
                     LLLDDVLAELDLSRQGMLMELVKADMQTLITTTHLDGFRPDWLVGAQITSVEAGVLSS
                     VGEIG"
     gene            complement(1675..2661)
                     /locus_tag="PKW73_08265"
     CDS             complement(1675..2661)
                     /locus_tag="PKW73_08265"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNA73318.1"
                     /translation="MKYYEDILEVFYEPQPGLIDRSAIALGFFDGVHPGHQVVISRAV
                     SEASRLKARSGVVTFKDHPRGLTRGQAPLLLSVIEERLSLFDQMGVDFALVLTFSEEL
                     CRLSPSQYVETMLVECLGARSISVGHNHHFGRDREGDAKLLERIGKEKDFVVHAADMV
                     YVDGLEVSSSRIRDLVIGKNLDLAARLLSRPFSLRGTVVRGDMRGRTLGFPTANVQHA
                     FNQLVPPCGVYAGFAQVLDEPGEKRLCVINIGVRPTFKNPELKISMGTTEPILTVEAH
                     LLDFDGDLYDKPMKLEFHKHLRDERKFDNLDALKAQIGEDCSMAKNSLPCLI"
BASE COUNT          702 a          807 c          717 g          651 t
ORIGIN      
        1 tgattcatac ggcaggaggc tttgccttcc cgcaagaaac gttgcaccga ctgagaatcg
       61 gcaatatgct gagactgcaa catcttgatg gcgacggtgc gctccatcaa gacctggcgt
      121 cctttataga cgacacccat gccgccgtga ccgataacat cgataatgtc gtacttgccc
      181 atcaggcaag taccgatcat aggatctctg gccagtgcaa caagagccgc cccgtcatgg
      241 gggcaggctt tgacgacgcc tgtgaactgt ctgttgcatt ccaggcagat cttttgatct
      301 agaccatcat gaagatttcc ggcgtgcact tgatcccttc gatttacttg caaaaagttg
      361 actgactaaa attgtattgt ggaacttgcg ccaatttgag tttcggggga gaaaaggcgg
      421 cgatatttga aaaaagccca ccttaactca tactaatgta ccccatacta tctctatcta
      481 accaatttcg ccaactgaag atagcacccc tgcctccacg ctggtaattt gagcaccaac
      541 aagccagtcc ggacggaagc catccagatg ggtagtggta attaatgttt gcatgtcagc
      601 cttgacaagc tccataagca ttccctgcct ggataaatcc aactcggcca gcacatcgtc
      661 cagaagaagc agtggcggct cgctcaaagc ttcttccacg agcttcaatt cggccagttt
      721 gagagccagc acaagagacc gttgctggcc ctggctggcg aaggcatggg cggcaaagtc
      781 attgatggca aagacgatat cgtctcgatg agggcctgtg agagtctgct tacgcaccac
      841 ttcctcggcg cgcctgtcct tcaaattcaa ataaattcgt ttggccaggt cctcttcttt
      901 catagccagc agttcagcca gacgcagatt ggcatcctcc tccccgtctt cttccacatc
      961 tctttgcttg accctgaaga gatagacggc ggaaagcctc tccttcagtc cggaaaccct
     1021 ggtcaaatta gcttcggcct ggggcaagag cttctccagc acggcggcac gcgccttcac
     1081 cagacgagcg ccgacccgag ccagttgtat atcccagact ttgatctctt cttcatccgg
     1141 ggcctgtcct ctttcaaaac aaagacgcag aagcttgttt ctttgctgca ctatcttttc
     1201 gtactggctt accacttccg caaaaccggg acggagcttg accagcactt gatccagcca
     1261 gtcgcgccgc tcctttggtc cgccccgtac cagggccaaa tcccgggtgc tgaagctgac
     1321 ggcggtaaga cgactcaagc cgcccagttc ggaacggctt accttcatgc cgttcacctt
     1381 caagaccctg ctcaggcgtg gcttcaagga gagtatcaag gagactgtct cctcgatacc
     1441 ctggcagaca taggtgattt caatgagggt gctctcggcg ccggcgagaa tgagatctcg
     1501 gtcggaagtg gcacggccgc tcttgatatt acagagtagc tcgatggctt ccagaagatt
     1561 ggtctttccc tgggcattgt cgccgatgag cagattgcgc accggatgca aggaaagact
     1621 ggcatcctgg tagtttctga aattcttgac agtaagccgg gagacaaaca cagtctaaat
     1681 cagacagggc aagctgttct tagccatact gcaatcctcg ccgatctggg ctttgagggc
     1741 gtcaagattg tcgaatttgc gctcatcacg caaatgcttg tggaactcca gcttcatcgg
     1801 cttgtcataa agatcgccgt cgaaatcgag caggtgcgcc tccacggtca aaatcggctc
     1861 cgtggtgccc atggaaattt tcaattcggg atttttaaag gtaggacgca cgcctatgtt
     1921 gattacacac aaacgttttt caccgggctc atccaggacc tgggcaaagc cggcgtaaac
     1981 accgcagggg ggcactaact gattaaaggc atgctgcaca ttggcggtag gaaagccgag
     2041 agttctgccc cgcatatcgc ctctgacaac ggtgcctctc agactgaacg ggcgggaaag
     2101 caagcgggca gccagatcca ggttcttgcc gatgaccaga tcacggatgc ggctgctgga
     2161 cacctccaga ccatcaacat agaccatgtc ggcggcatgg accacaaagt ctttttcttt
     2221 gcctatgcgc tcaagcagct tggcatcacc ttcccggtcc cgtccgaaat gatggttatg
     2281 ccccacggaa atcgacctgg cacccagaca ttccaccagc atagtctcca catactggct
     2341 aggagaaagg cggcagagct cttccgagaa ggtgagcacc agcgcaaaat ccactcccat
     2401 ctggtcgaat aaactcaatc tttcctcaat gacagaaagc aggaggggtg cctggcctct
     2461 ggtcagtccc cggggatgat ccttaaaagt gaccacgccg gagcgggcct tcagtcggga
     2521 agcctcactg acagcccggg atatcaccac ctggtgacca ggatgaactc cgtcaaaaaa
     2581 tcccagagca atggcggaac ggtcaatcaa tccaggctgg ggctcgtaga aaacttccaa
     2641 gatatcctcg taatacttca tgggggaaag atccccttgg tctttacaaa ttcaaagggc
     2701 ggaccctctg aatcataact gggattttag cccctaaccc cccttctcgt cactgtaaaa
     2761 acggcgtgag ataggcttct gtgctaaaat ttgcctgtta agaggagtcc tatctaacag
     2821 ggactgaaat tacctccacg gcggatattc agaagatatc ccccgacggg cattttc
//
LOCUS       DAOQNZ010000405         2876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18674_length_2876_cov_2.967387, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000405 DAOQNZ010000000
VERSION     DAOQNZ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18674_length_2876_cov_2.967387"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..689
                     /locus_tag="PKW73_08270"
     CDS             207..689
                     /locus_tag="PKW73_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73319.1"
                     /translation="MQTGDTAAEDGGGAGGTAGNLSSLSLIKDASSSCSYLIRGLYKV
                     ARGWQERGELNAARRIYKFILKIKERSQVNCLEVVMSQANLALIYLKEEAYPEAEEFF
                     KRALSACYELLGEESEVFNTLLSDYAALLRKMGLPEQAHYLEARNWSKPGGLPFLLSS
                     "
     gene            complement(679..>2876)
                     /locus_tag="PKW73_08275"
     CDS             complement(679..>2876)
                     /locus_tag="PKW73_08275"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNA73320.1"
                     /translation="LGRPLTLAEKVLTAHLDHYPEGELKRGESYALLRPDRVAMQDAT
                     AQMALLQFMQAQLKKVAVPSTVHCDHLIQARDGADKDMERACDENKEVYDFLASASNK
                     HGIGFWQPGAGIIHQVVLENYAFPGGMMIGTDSHTPNAGGLGMIAIGVGGADAVDVMA
                     GEAWGVRWPKLIGVHLKGKLNGWTSPKDVILKLAGILTVAGGTGAIIEYFGEGTKSIS
                     CTGKGTITNMGAELGATTSIFPYDERMAAYLKITGREEIAKLADAHKDELTADAEVAK
                     EPNKFFDQVVEIDLDTLEPYVVGPHTPDLARPISAFAAEIKEKGYPDKLSYALIGSCT
                     NSSYEDMSRAAHVAKQGVEAGIKSTCGFLVTPGSEQIYETIQRDGQMKTLNDIGAVVM
                     ANACGPCIGQWKREDIKTGEKNSIITSFNRNFQKRNDGNAETLAFIGSPEIVTAFALA
                     GSLSFNPITDTITTESGKKVKLAPPTADELPGKGFVCENKGYIAPAADGDKVSVIVSK
                     DSERLQLLEPFAAWNGKDYKQLPVLMKALGKCTTDHISAAGPWLRYRGHLDRISDNMF
                     IGATNAFTKETGVGIDVLSGEKGSYPQVARHYKAEGMGWVAIGDTNYGEGSSREHAAM
                     SPRFLGCKAVIVKSFARIHETNLKKQGILALTFVNPSDYDKIQEKDRISLLGLSALAP
                     GKTVEMLIEHEGGKSETIEVKHTMTEEQIGWFKAGSALNLIREKQMAKS"
BASE COUNT          595 a          756 c          834 g          691 t
ORIGIN      
        1 gtcgtagagg aaggtctcca taacgatctg gtagcaaatc accccaagct catgaccgat
       61 gctttaaacc ggttactggc aagaacccgg tcgggcaaag atatggggta gccccggata
      121 agttatctgc tcacccctct tggcaagaat cgaatttggc cttttactta agatgtccgt
      181 tcttatgggc aggccgggag ggttatatgc aaactggaga cacggcggct gaagacggcg
      241 gcggtgccgg tgggaccgct ggtaatttga gcagcctcag tctgatcaaa gacgccagtt
      301 cgtcttgttc ttacctgatc cgaggccttt ataaggtggc tcgcggctgg caggagcgcg
      361 gcgagttaaa cgccgccagg cgcatctaca agtttatttt gaaaatcaaa gagcgctccc
      421 aagtcaattg cctggaagtg gtgatgagtc aggctaactt ggctcttatc tatcttaaag
      481 aagaagccta tcccgaggcg gaagaatttt tcaaaagagc gctttcagcc tgctatgagc
      541 ttctgggcga agaaagcgaa gtcttcaata cgcttttgag cgactatgca gcgctcctta
      601 gaaaaatggg cctgccggag caggcccatt acctggaagc gcgcaactgg tctaaaccag
      661 gcgggctgcc gtttcttctc agctcttagc catctgtttc tcacgaatga ggttgagagc
      721 cgaaccggct ttgaaccagc cgatctgctc ttccgtcatg gtgtgcttca cttcgatggt
      781 ctcgctcttg ccgccttcgt gctcgatgag catttccaca gtcttgccag gggcgagagc
      841 tgaaagccca agcaagctga tgcggtcttt ctcttgaatt ttgtcgtagt cggaaggatt
      901 gacaaaagta agagcgagga tgccctgctt cttcaggttg gtttcgtgga tgcgggcaaa
      961 gctcttcacg atgacggcct tgcaaccaag gaagcggggc gacatggccg catgctcacg
     1021 actggagcct tcgccgtagt tggtgtcgcc gatagccacc cagcccatgc cctcggcttt
     1081 gtagtggcga gccacttgcg ggtaagagcc cttttcgccg gacaagacat cgataccgac
     1141 gccggtctct tttgtgaagg cattggtggc accgatgaac atattgtcag agatgcggtc
     1201 caggtgacca cggtaacgca gccagggacc ggcggcggaa atatggtcgg tggtgcactt
     1261 gcccagggct ttcataagca ccggtagttg cttgtagtct ttaccgttcc aggcagcgaa
     1321 aggttccagc aactgcagtc tttcagagtc tttgctgacg atgaccgaga ctttgtcgcc
     1381 gtcggcagcg ggagcaatat agcctttgtt ctcacaaaca aagcccttgc ccggcagttc
     1441 gtcggcggtg ggaggcgcca acttgacctt cttgccgctt tcggtggtga ttgtgtcggt
     1501 gatgggattg aaagaaaggg aaccggccag agcgaaagcc gtgactatct cggggctgcc
     1561 gatgaaggcc agggtttcgg cattgccgtc gttacgcttc tggaagttgc ggttgaagga
     1621 agtgatgatg gagttcttct ctccggtctt gatgtcttca cgcttccact gaccgatgca
     1681 tggtccgcag gcattggcca tgaccacggc gccgatgtcg ttcagggtct tcatctgacc
     1741 atcacgctga atggtctcgt aaatctgctc ggaaccgggt gtgaccagaa aaccgcaagt
     1801 gctcttgatg cccgcttcaa ccccttgctt ggccacatgg gccgctctgc tcatgtcttc
     1861 ataggaagag tttgtgcaac tgcctatcaa ggcgtaggag agtttgtccg gatagccttt
     1921 ctctttgatc tcggcagcga aagccgaaat aggacgggcc aggtcggggg tgtgaggacc
     1981 cactacatag ggttccagag tgtccaggtc gatctccaca acttgatcga agaacttgtt
     2041 gggctctttg gctacttccg catcggcagt caattcgtct ttgtgagcgt cggccagttt
     2101 ggcgatttcc tcacggccgg taatcttcag ataagcggcc atgcgctcgt cataagggaa
     2161 gatagaggtg gtagcaccca gttcggcacc catattggtg attgtgcctt tgccggtgca
     2221 ggaaatagat ttggtgcctt cgccgaagta ttcgatgata gcccctgtgc caccagctac
     2281 ggtgaggatg ccggccagct tgagaatgac gtctttaggg gaagtccagc cgttcaattt
     2341 gcctttcagg tgtacgccaa tgagcttggg ccatctcacg ccccaggctt ctccggccat
     2401 cacatctaca gcgtcggcgc cgcccacccc gatggcaatc atccccagac caccggcatt
     2461 aggggtgtga gagtcggtac cgatcatcat gccgcccggg aaagcgtagt tttccagcac
     2521 tacctggtgg ataatgcccg ctcccggctg ccagaaaccg atgccatgtt tgttggaggc
     2581 ggaagccagg aagtcgtaga cttctttatt ctcgtcgcag gcccgctcca tgtctttgtc
     2641 ggcgccgtct ctggcctgaa tcaagtggtc gcagtgtacg gtgctgggta cggcaacttt
     2701 cttcaactgg gcttgcatga actgcagaag ggccatttga gcagtggcat cttgcatggc
     2761 gacgcggtcg ggacgcaaga gggcatagct ttcaccccgc ttcaattcgc cttccggata
     2821 gtgatccagg tgggccgtca gtactttctc cgccagagtc agggggcgac ccagtt
//
LOCUS       DAOQNZ010000406         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18696_length_2874_cov_3.718340, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000406 DAOQNZ010000000
VERSION     DAOQNZ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18696_length_2874_cov_3.718340"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKW73_08280"
     CDS             complement(<1..220)
                     /locus_tag="PKW73_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73321.1"
                     /translation="MKTERHQQFKLVMGLLAVVAVVGAAIFAPAWFAAKSNAPPVIIE
                     QSMEPQSAATPEKSHAPEVAAAVTRVEKV"
     gene            complement(247..1080)
                     /locus_tag="PKW73_08285"
     CDS             complement(247..1080)
                     /locus_tag="PKW73_08285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNA73322.1"
                     /translation="MDNPQPDSDPDSLTFPLGPVSPGLLVGALLGAFAVTFAGAALLE
                     PLIVWFNFSDWMLAPALNQLLNLLVTAMPAVFLYLVILPQVPHLNSGMQIPSRSEDWF
                     RILSFSLLGIGLVLLADFVTSGFGTSTNSALAVPYGGMEGQVEELRQYQQGHGGAPLP
                     AHYASFFGLNELVLSAILVLGSPLIEEYICRGVFLPLFHGFFDGRLTSFGAYARPFNC
                     LFSVVLSALLFALPHAEAVFSQFLFGLIVGWLYLRSGNIWSSVLVHVMNNALVAVVYF
                     A"
     gene            complement(1196..1510)
                     /locus_tag="PKW73_08290"
     CDS             complement(1196..1510)
                     /locus_tag="PKW73_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73323.1"
                     /translation="MKALFTSASFLSAFQGFRSCFKWLLAFLLFFSLSGCAGLGVSYY
                     PGGGYYRGGGYYRGGGYYNGGGGYYNGGGYRQQQHCYQSLRPYYDRWGNYLGQRYVTV
                     CN"
     gene            complement(1516..2217)
                     /locus_tag="PKW73_08295"
     CDS             complement(1516..2217)
                     /locus_tag="PKW73_08295"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA73324.1"
                     /translation="MSSPNTDDTVKFPIATAYIVVVTFVFHLLVQAGIISGQLLALIP
                     LRFQDMQLDYGSIIAFLFTLPNLATYALVHSGWGHLLGNLAFLIVLGPSLERALGART
                     YMAIYVFGSVVAGLFALAGDYVYSVGADGTTTAVIIVGASASISAVMGGVLLKTPMEI
                     IGVLPFVNFVIRAWMAVVMFIAEQLVVINTAGMGNAPIGSATLHLSGIGAGMFFIMIM
                     PTALSAYEKFKNSRS"
     gene            complement(2241..>2874)
                     /locus_tag="PKW73_08300"
     CDS             complement(2241..>2874)
                     /locus_tag="PKW73_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73325.1"
                     /translation="GEDRLAIDIGSFDSYSVRLQYMPTENWALGTSYAHVRNPEASHP
                     GDVDRFTAFALYSKRFDQGYGSVSAVFGRNLEQHGTLTGGLVEGTLNFEDRHYLYGRV
                     EYVDKPGLTGVNIFGQPGLSSIVAAEHDEHLEGGHGAAEGNTEHGAAGISLVGAFTFG
                     YAYDVYASSALRIGLGADVTFYDVPTQLKEVYGDPVSFMLNLRVRPGRSF"
BASE COUNT          795 a          775 c          775 g          529 t
ORIGIN      
        1 gtaccttctc cacacgagtt actgcggcag ccacctcagg agcatggctc ttttcaggcg
       61 tggcagcgct ctgcggctcc atcgactgtt cgatgatgac gggaggagcg ttgctcttag
      121 cagcaaacca ggcaggcgca aaaatagcag cccccaccac ggcaacaact gcaagcagcc
      181 ccatcaccag tttgaattgc tgatgtcgtt cagttttcat agttctgtga acctcttaag
      241 tttgcatcag gcgaagtaaa ccaccgccac cagtgcgttg ttcatcacat gcacgaggac
      301 ggaagaccag atattgccgc tgcgcagata aagccagccc acaatcagac caaagaggaa
      361 ctgactgaag acggcctctg catgcggcaa ggcgaacagc agtgccgata gcaccaccga
      421 gaacaggcaa ttgaacggtc tggcataagc accgaagctc gtcagccgac cgtcgaagaa
      481 gccatgaaag agaggcaaga aaacaccccg acagatatat tcctcgatca aaggtgagcc
      541 cagcaccaaa atggcagaga gcacaagctc attcaaaccg aagaatgagg cgtaatgtgc
      601 cggcagaggc gctccaccat gaccctgctg atactgcctc agttcttcca cctgtccttc
      661 catgcctcca tagggcacgg ccagggcgga attggtcgag gtaccgaagc cgctggtgac
      721 aaaatcagcc agcaagacca ggccgatgcc taacaggcta aagctcagaa ttctgaacca
      781 gtcctcactt ctactgggta tttgcatacc gctattgaga tgaggtacct ggggcaaaat
      841 gacgaggtat aagaagaccg ccggcattgc tgttaccagc aagttgagca gttggttgag
      901 ggcgggcgcc aacatccagt ctgagaagtt gaaccagaca atcagcggtt caagcaaagc
      961 agcaccggcg aaggttacgg caaaagcacc cagcaaggcg cctacaagca agcctggaga
     1021 caccggacca agggggaaag ttaagctatc aggatcagaa tcgggttgag gattatccac
     1081 taaaaacctc caggtcagtt agacagcttt atgaatggat ggatggattg aatgattaga
     1141 ctgactgtcc aggcggagaa gcccgtgctt ctccgcccga gaacaacgtc ggcagttaat
     1201 tgcaaactgt gacgtagcgt tgccccaggt aattgcccca ccggtcatag taaggacgca
     1261 gagactggta gcaatgctgc tgctggcgat atccgccgcc gttgtagtag ccgccaccac
     1321 cgttgtagta gccgccgcca cggtaataac caccaccgcg gtagtagccg ccaccgggat
     1381 agtagctgac gcccagaccg gcacagccgc tcaagctgaa gaagagcaga aaggccagca
     1441 gccatttgaa gcaactgcgg aagccttgaa aggcgctaag gaaagaagca ctggtaaata
     1501 atgctttcat gagatttaac tccgtgagtt tttgaatttt tcgtaggcac tgagggcggt
     1561 gggcatgatc ataatgaaga acatgccagc gccaatgccg gacaggtgca gggtggccga
     1621 accaatcggg gcgttaccca taccggcggt attgatcacc accaattgct cggcaatgaa
     1681 catgactact gccatccagg cccgaatgac aaagttgaca aagggcagaa cgccgattat
     1741 ctccatggga gttttcagca aaaccccgcc cataactgct gaaatcgaag cagaggcccc
     1801 gactatgatg acggcggtgg tagtaccatc ggctccgacc gaatagacat agtcaccggc
     1861 gagagcgaat aagccggcta ccacggagcc gaagacataa atcgccatat aggttctagc
     1921 ccccagagca cgctccagcg acgggccaag tacgatcaag aaagccaggt ttcctaaaag
     1981 gtgtccccag cccgagtgca ccagagcata ggtagccagg ttaggcaggg tgaagaggaa
     2041 agcgatgatc gagccgtaat ccagctgcat atcctggaac cggagtggta tcagcgccag
     2101 aagctgtccg ctgataatgc cggcctggac aagaaggtga aaaacaaagg tcactaccac
     2161 gatgtacgca gtagcaattg ggaatttgac tgtgtcgtca gtgttaggag aagacataaa
     2221 gcctccttaa aaaattactg ttaaaaactt cgacccggac gtacacgcag attgagcatg
     2281 aacgaaaccg gatcaccgta tacttctttg agctgggtcg gcacatcgta gaaggtgaca
     2341 tcagccccca gaccaattct taatgcgctc gatgcataga catcgtaggc atagccgaag
     2401 gtgaaggcac ccactagaga gattccagcg gcaccatgct cggtattacc ttcagcagcg
     2461 ccgtgaccgc cctccagatg ctcatcgtgc tcggcagcca cgatagagga aagaccaggc
     2521 tgtccgaata tgtttacgcc ggtcagacca ggcttatcca catactcaac ccgtccgtag
     2581 agataatggc ggtcctcaaa gttcaaagtg ccctccacca gtccgcctgt gagagtgccg
     2641 tgctgttcga gattgcgccc gaagacggca gagaccgagc cgtagccctg atcaaagcgc
     2701 ttgctgtaga gagcaaaggc ggtgaatctg tccacatcgc cgggatgact ggcttcggga
     2761 ttgcgcacat gtgcgtaaga tgtcccgagc gcccagttct cagtcggcat gtattgcaga
     2821 cgcaccgaat agctatcgaa agagccgata tcaatagcca ggcggtcttc accc
//
LOCUS       DAOQNZ010000407         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18743_length_2871_cov_3.954190, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000407 DAOQNZ010000000
VERSION     DAOQNZ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18743_length_2871_cov_3.954190"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PKW73_08305"
     CDS             complement(<1..1041)
                     /locus_tag="PKW73_08305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNA73326.1"
                     /translation="MTEVVDSVYSAAIDLWIEVGSAYENPENSGVSHCIEHMLFKGTK
                     NRTSERLMEEIEDVGGMLSASTSRELTKLYGHVLGESLPVAVDIILDMAKSPLMAAED
                     LELERKVILDEIEMYEDDPGDVAQELVYSRMWEGSPQAMPITGDARAVKKISVEMLQE
                     HFQKYFRPERMIVSVAGSFDEDATIAALSQGLGSLEGSASKAYERPTVPEVKAFKELI
                     DWDTEMAQLIMAMPGLSTLHRLQPALLVLDLCLTTSASSRLFKEVREKRGLAYNIGSL
                     QHSYRDSGLYGVAAAVSPAKAEAVLELVLAELALLKKEGLSEREILRAKSQLRTDLLM
                     DKESMSARSTGNA"
     gene            complement(1632..2324)
                     /locus_tag="PKW73_08310"
     CDS             complement(1632..2324)
                     /locus_tag="PKW73_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73327.1"
                     /translation="MSDNKDGLKGETGGQAHKSSQEKSGAATQEVKPDWMQVVQKGTK
                     AALKDLIYDANDMLEPVAKAMRSLQHKDAGVDALKLAGWAQVDLKLETSPLHKLLENK
                     PVDSYNCKYYVKGYIEGRLPPGDGRREEMDPEYLKSKGYEPVKDHRFQPGDIVMVRTL
                     AGNSAVRYAHVALVEKVAPVTGNILSFIQKPDVEHPVSRCSKAAFDAAYKVDGVNAAC
                     EIYRKYDSNGHK"
     gene            2449..2521
                     /locus_tag="PKW73_08315"
     tRNA            2449..2521
                     /locus_tag="PKW73_08315"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2482..2484,aa:Thr,seq:cgt)
     gene            complement(2543..>2871)
                     /locus_tag="PKW73_08320"
     CDS             complement(2543..>2871)
                     /locus_tag="PKW73_08320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNA73328.1"
                     /translation="RSGLKFKDLKQGDGARPQTGKTVVVNYTGWLTNGQKFDSSLDRG
                     EPFTFAIGKGNVIKGWDEGVKSMHIGGKRRLVIAPQLGYGASGAGEAIPPNATLVFEV
                     ELLGIK"
BASE COUNT          634 a          821 c          669 g          747 t
ORIGIN      
        1 ggcattaccc gtgctgcgcg cactcatcga ttctttgtcc ataagcaaat cggtgcgcaa
       61 ttgagacttg gctctcaata tctcacgctc actgagcccc tctttcttca gtaaagcgag
      121 ctcagccaga accagttcaa gcaccgcctc ggccttagcc ggactgacgg cggcagccac
      181 gccgtataaa ccggaatcac ggtaagaatg ttgcagcgag cctatgttat aagccagtcc
      241 ccgcttctct ctcacttctt tgaaaagccg cgaagaggct gatgtagtaa gacataaatc
      301 gagcaccagt aaagctggtt gcagtctgtg caaagtagac agtccgggca tggccattat
      361 caactgggcc atttcggtgt cccaatcgat cagttccttg aaagccttaa cttccggcac
      421 ggtcggtcgt tcatatgctt ttgaggcact gccttccaga ctgcctagcc cctgggagag
      481 agccgcaatg gtggcatctt cgtcaaaact gccggccacc gatacaatca tgcgctcagg
      541 tctgaaatac ttctggaagt gttcctgcaa catttccacc gagatcttct taacggcccg
      601 ggcatcgccg gtaatgggca tggcctgagg cgatccctcc cacatgcgag aataaacaag
      661 ctcctgggca acgtcgcctg gatcgtcttc gtacatctca atttcatcga gaatgacttt
      721 ccgttccagt tccagatctt ctgccgccat aagcggtgac ttggccatgt ccagaatgat
      781 atccacagcc acaggcagag actctcccag cacatggccg tacaacttgg tcaactctct
      841 ggaagtagaa gcgctgagca taccgcccac atcttcaatt tcttccatga gcctttcaga
      901 ggtgcggttt tttgtgccct taaagagcat gtgctcaata cagtgagaaa ccccgctatt
      961 ttcagggttc tcataggctg aacctacttc tatccagaga tcgatggcgg ccgaatagac
     1021 cgaatcaacg acctcggtca ctattcttgc cccgttgggg agggttgttg tccgaatcat
     1081 tttatgcttt tacaggaagc tcatattcta gcacctgccg gaccttgagc cagctttata
     1141 gcgcttaagg taccaatttg agtttggctt tcggtttgaa aagctatttt ggaacttcct
     1201 tgcgtgcctg ccaacaaaaa caccgcccgg ctgagaccgc ggcggcgttt ttgctactgt
     1261 ctggctgccc ttgcggggca cagtatttca ttgcgctgga gccagtctct cacgcaatgc
     1321 gggctatctg cttttgtttc cgacttggcg cctatctggc gtttccgcac tgcccctcag
     1381 ctttcgcggt cacttcgctt aaccactcac cgttagtatt tctactctag ctctgccggc
     1441 cggggctggg aaggtttgag aagctttagt ttaagactag atcacattac gcaatgccac
     1501 agcccgtgcc cattacaggc gaaattgccg tttttcgaaa cacaagctta acgggagaag
     1561 accgcgctcc tatccgttat tttaccgttt gccaacaatc taacagagag caaacatcaa
     1621 agctaaagcc tttatttatg cccattagaa tcatattttc tatatatctc gcaagcggca
     1681 ttcaccccgt caaccttgta ggcggcgtca aaggcagcct tactgcagcg gctaacagga
     1741 tgctcgacat ctggcttttg aatgaagctg aggatgttgc ccgtcaccgg cgccaccttc
     1801 tctaccagcg ccacatgggc atatctgacg gcagaattgc cggccagggt tctaaccata
     1861 acaatatcgc cgggctggaa gcggtggtct tttaccggct catagccttt actcttgagg
     1921 tattccgggt ccatttcctc ccggcgaccg tctccaggcg gcaggcgccc ctctatatag
     1981 cctttaacgt aatacttgca gttgtaactg tccacaggct tgttttccag aagcttatgg
     2041 aggggcgagg tttccagctt caggtcaacc tgcgcccagc cggcaagctt gagggcatcc
     2101 accccggcat ccttatgttg taaggagcgc atagccttag ccaccggctc aagcatgtcg
     2161 ttggcgtcgt aaatcaagtc tttcagggca gcctttgtgc ctttttgcac tacctgcatc
     2221 cagtccggct tgacctcctg ggtagccgcc ccagacttct cttgggaact cttgtgtgcc
     2281 tgtccgcccg tttcaccctt aagcccgtct ttgttgtcgc tcatagctgc gttaacccac
     2341 agtaagtgcc ttcttctcta actttaccgc ctggagatac cgaagtatgt agagatccgt
     2401 agattttcat ggagatcccg agagctatct tttataattg gaattcctgc cgatgtagct
     2461 cagctggtag agcaacggtt tcgtaaaccg taggtcgtgg gttcgaatcc cgtcatcggc
     2521 taagttttaa aattgatacc gtttacttga taccaagcaa ctcgacttca aagacaaggg
     2581 ttgcattggg aggaattgcc tcgccggcgc cacttgcccc gtagcctagc tggggcgcaa
     2641 tcaccaatcg ccgcttccca ccgatgtgca tactcttcac accttcgtcc cagcccttaa
     2701 tgacgttgcc cttaccaatc gcaaaagtaa acggctcccc gcgatctagc gaactatcga
     2761 acttttgccc gttagtaagc caaccggtgt aattaacaac aacagtcttc cccgtttgcg
     2821 gacgagcgcc atcgccctgt ttcaagtctt tgaatttcaa accgcttctg g
//
LOCUS       DAOQNZ010000408         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18768_length_2870_cov_3.316874, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000408 DAOQNZ010000000
VERSION     DAOQNZ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18768_length_2870_cov_3.316874"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKW73_08325"
     CDS             complement(<1..568)
                     /locus_tag="PKW73_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73329.1"
                     /translation="MLFAETMAIRWLGIEIPVLRAFPNLILMLVFIGASAGIAQPDRK
                     VSKVKLTCALLCLIGSVIIVPITGLKDLSLRIEENNSTAAVIAGLTLISLNALSLLTI
                     FKEIGTTVGEGFKNRPPLSAYSINLLGSMFGVLAFAACSFLNTPPWVWLLIAGLGTFA
                     YNRSRALLLFLAPLVVASYFSAQGAFWSP"
     gene            complement(600..1937)
                     /locus_tag="PKW73_08330"
     CDS             complement(600..1937)
                     /locus_tag="PKW73_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNA73330.1"
                     /translation="MSASQRLYLLAILAIVPMAAAAWSAELVKIGLALDLMLLLAFLI
                     DRQITPPPSTIDARRDMDDRLSIGRDNDVVLTISYKGGIPISCLVKDDSPEGIEKSAS
                     LLSVQLEPGQETRAAYRLSPRRRGSYQFGQINLRYRSRLGLFYYERKIQAQAKIKVYS
                     DLKALHDLSIKLSHSSELGELHQRKRGQGTDFASLKEYSSGDDCKAIDWRATARRDRP
                     VIRTYEAEQEQRLLILVDAGRMMVSDLEGLSRFDRALNAALCLALTGLSHNDQVGIGI
                     FADKPLLYLPPKRGKPYMHRILEGTFAVEPRMVEPDYAGILSYFASAQKGRSLMVVLT
                     DLTDPTGSQALLSGLANLAPRHLPFCVTLKDRQVQKFASQDLVAAEKSSAHKGDIINK
                     IYQKAVALDLTSQRELALSVLTRRGCLVLDAAPQELSDRLVEKYIEVKSRGRL"
     gene            complement(1934..>2870)
                     /locus_tag="PKW73_08335"
     CDS             complement(1934..>2870)
                     /locus_tag="PKW73_08335"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNA73331.1"
                     /translation="TRATFARLKDALNETIVGQPMVIDQLLTAILADGHVLLEGVPGT
                     AKTLLVKSIASLVGADFGRIQLTPDMLPSDIVGTSVYDLNARTFSLRKGPIFTSLLLA
                     DEINRTPPKTQSALLEAMEERQVTLDGHTEKLPELFLVVATQNPVEFEGTYPLPEAQL
                     DRFMLKVLIGYPGAAAEKQMLSNWQEGKYRRFTKPLTAVTSAEEILQCRQELLKVKVE
                     ASIMDYLVELVQKSRSISDLQLGASPRAALAWLAAAKAHAAIEGRDFVTPDNIKFVAE
                     PVLRHRLIMTAEAELDGVTVSQVVANLLRQVTVPR"
BASE COUNT          610 a          744 c          861 g          655 t
ORIGIN      
        1 agggtgacca gaaggctccc tgggcgctga agtaacttgc tactactagt ggtgccagga
       61 aaagtagcag ggcgcggctg cgattgtagg caaatgtgcc cagtccggct atcaaaagcc
      121 agacccaggg cggtgtgttc aggaaggagc aggcggcaaa ggccagcact ccgaacatgg
      181 agccgagcaa attgatggaa taggctgaga ggggcgggcg atttttgaaa ccttctccga
      241 ccgtggtgcc aatctccttg aagattgtca gtaagcttaa ggcgttcagt gaaatgagcg
      301 tcagtccggc tatgacggct gccgttgaat tattttcttc gatgcgcaga gagaggtctt
      361 tgagacccgt tatgggcacg attatcaccg agccgattaa gcaaagcagg gcgcaggtaa
      421 gtttcacttt ggaaactttt ctatcaggct gtgcaatgcc ggcggaagcg ccgatgaaga
      481 ccagcatcaa gattaagtta gggaaggctc gtaaaaccgg aatctctatg cccaaccagc
      541 gaatcgccat ggtctcagca aagagcatca cgaaacttgc gaagaacagg ttactcagct
      601 tacaatctcc cccttgattt gacctctata tatttttcca caagtcgatc cgataattcc
      661 tgtggagcgg cgtccagcac caggcagcct cgccgcgtca gtacgcttaa agctagctcc
      721 cgctgactgg ttaggtctag tgctactgcc ttttgataaa tcttgttaat gatgtcgcct
      781 ttgtgcgccg atgatttctc tgccgccacc agatcttgcg aggcgaattt ttgaacctgt
      841 ctgtccttga gtgtcacgca gaagggcagg tgcctggggg cgagattggc caggccgctc
      901 aggagggctt gggagccggt ggggtctgtc aggtcggtga gcaccaccat cagggagcga
      961 cccttctggg cggaggcgaa ataggagagg ataccggcgt agtctggttc gaccatgcgc
     1021 ggttccactg caaaagtacc ctccagaatg cggtgcatgt aaggcttgcc gcgcttgggc
     1081 ggcaggtaga gcaggggttt gtcggcgaag atgcctatgc ctacctggtc gttgtggctg
     1141 agtcctgtaa gcgcgaggca gagagcggca ttgagcgccc tatcgaagcg gcttaaacct
     1201 tccagatcgg aaaccatcat tctgccggca tccaccagga taagcaggcg ctgttcctgc
     1261 tcggcttcgt aggtgcgaat caccggccga tcacgcctgg ccgtggcgcg ccagtcgatg
     1321 gccttgcaat cgtctccgga gctgtattcc ttcagggagg cgaagtcggt gccctgcccg
     1381 cgcttgcgct ggtgcagttc gcccagttcg gaggaatgcg agagtttgat ggataaatcg
     1441 tgcagcgcct tcagatcgga ataaactttg atcttggctt gcgcctggat ttttcgctca
     1501 tagtaaaaaa gtcccagtcg gctgcggtag cgaagattga tctgaccaaa ttgatagctg
     1561 cctctgcgcc tggggctcag tctgtatgct gcccgcgttt cttgaccggg ttccagttga
     1621 accgacaaga gcgaggctga cttttcaatg ccctccgggc tgtcatcttt taccagacaa
     1681 gagataggta tgccgccttt gtagctgatg gtgagcacca catcgttatc ccgaccgatg
     1741 gacagcctat cgtccatgtc gcggcgggcg tctatggtac ttgggggcgg cgttatctgt
     1801 ctgtcgatga gaaaagctag aagcaacatc agatcgaggg ccagtccgat tttgaccagc
     1861 tcggccgacc aggctgccgc agccattggc acaatcgcca ggattgccag aagatagagt
     1921 ctctggctgg cgctcatcgc ggcactgtca cctggcgcag caggttagcc actacttgtg
     1981 ataccgttac accgtccaat tccgcttctg ccgtcatgat caagcggtgc cgcaaaacag
     2041 gctctgccac aaactttatg ttgtcgggag tgacaaaatc tcgtccctct atggcggcgt
     2101 gggctttggc cgccgccagc caggccaggg cagcgcgggg gcttgcaccc aattgcaggt
     2161 cggagataga acgagacttc tgcaccaact caaccaggta atccatgatg ctggcttcta
     2221 ctttgacctt gagcagttcc tggcggcact gaagaatttc ttccgccgag gtcacggctg
     2281 tcagaggttt agtgaagcgc cggtacttgc cctcctgcca gtttgagagc atttgctttt
     2341 ctgcggcggc gccgggatag ccgatcaaca ctttgagcat gaagcggtcc agttgggctt
     2401 ccggcagtgg ataggtgcct tcgaactcga ccgggttttg tgtggctacc acaagaaaca
     2461 attcgggcag tttctcagta tggccgtcca gggtcacctg tctttcttcc atggcctcca
     2521 gaagagctga ctgggtcttg ggcggggtcc ggttgatctc atcagccagc agtaaggagg
     2581 taaaaatggg acctttgcgg aggctgaatg tgcgggcatt caagtcataa acgctggtgc
     2641 cgactatatc ggagggcaac atgtcgggag tcaattgaat gcggccgaag tcggctccca
     2701 ccaggctggc aatgctcttc accagaaggg ttttggccgt gccaggcact ccctccagaa
     2761 gcacgtgacc gtcggcgagg atagccgtta gtagctggtc tatgaccatg ggttggccga
     2821 cgatagtttc gttgagggca tccttgaggc gggcaaaggt agcgcgtgtg
//
LOCUS       DAOQNZ010000409         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18779_length_2869_cov_4.639303, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000409 DAOQNZ010000000
VERSION     DAOQNZ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18779_length_2869_cov_4.639303"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PKW73_08340"
     CDS             complement(<1..902)
                     /locus_tag="PKW73_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA73332.1"
                     /translation="MRLKVDSGGGLQAVIYTLKKALEVGPLKLWQRMRSKNACKTCAL
                     GMGGMKGGMVNEAGHFPEVCKKSLQAQAADMQGAIAGDFFDKTGLSSLLQLTPKQAED
                     LGRLSYPIMHKAGSTHFQPITWEFAFEYIARQLKEAPTPDHVGFYASGRSSNETGFLL
                     SSFARIYGTNNVMNCSYYCHQASGVALKMTIGTGTATIALDDIDHCDMVFLIGANPAS
                     NHPRLMSKLTDLKERGGKVLVVNPVRELGLEKFRVPSKPKSMLLGTRIADLYLKPLAG
                     GDIALLTGILKTLLEEGKVQMDYL"
     gene            1078..>2869
                     /locus_tag="PKW73_08345"
     CDS             1078..>2869
                     /locus_tag="PKW73_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73333.1"
                     /translation="MKRKRVATMAKRLAVFSMLSTLYPLGLPAGAADVNSLLASVSSI
                     SDVDARQASIEKRLQDAYNAGRLTQAQYEGFKQALARVADQEAVFRASDSSLSLWESL
                     RLQFDIDRIVKEMEASLADRQVGFVDVFARRDEITKRLGVAYSDGRLTSQEYAQIQME
                     LENINKTIQVQSAANGHLSVADSIKLVLELDRLSQKLTRTVHDRQVSLTAIDVRQSEI
                     EKKIEGGVASGKLTDREAADLRGEFKRIAEREESLRKLGRPLSAEEQLTLALDLEKLN
                     GQVDAQVFDNEVQDNSAITMDKRRTDVDKNLAAALSAGLITLSEAQVYKDELDAVVKK
                     EESFKTASGGQLTLVQAQNLLIDLERLNGKIDRAVFGRKPVWVGIEGSIDDIRRRIAD
                     AKVGQRLAEVDEVELKSDLDKVIANKATYAASSAGIDSQEAISLAVDLTKLNGKMFKL
                     IKDREISVLPDLVKRKDEINKRMSDGTLSGKLTSDETRSLLEDFDRISAREAAFRATD
                     GILDEREKLSLALDLERLAARLEKEIRDNPYVSLPLSQKKTQADQIISTGTLTGKLSA
                     QEVQNLRAEYNRITAFEAASLASGSTHDAKE"
BASE COUNT          706 a          718 c          794 g          651 t
ORIGIN      
        1 ctcaggtaat ccatttgcac cttgccttct tccagcaagg tcttcaagat gccggtcagg
       61 agagcaatgt ctcccccggc tagaggtttc aagtagaggt cggcaatcct ggtgcccaga
      121 agcattgact tgggcttgga cggcacccga aatttctcca gccccaactc tcgcaccgga
      181 tttacaacca gcactttgcc gccgcgctct tttagatcgg tgagtttgga cattaagcgc
      241 gggtgattgc tggccggatt ggcgccgatc aaaaagacca tgtcgcagtg gtcgatgtca
      301 tccagtgcga ttgtggcggt gcctgtgccg atggtcattt tcagggcaac gccggaggct
      361 tgatggcaat agtaagagca gttcatgaca ttgttggtgc cataaatgcg ggcaaaggag
      421 gagagcagaa aaccggtttc gtttgatgag cgtcccgagg cataaaagcc cacatggtcg
      481 ggggtagggg cttctttcag ttgcctggcg atatattcga aggcaaactc ccaggtgatc
      541 ggttgaaaat gggtggaccc cgctttgtgc atgatcggat aactgaggcg ccccaggtct
      601 tcggcctgct tgggtgtgag ttgtagcaaa ctggaaagcc cggtcttgtc gaagaagtcg
      661 ccggcgatgg ccccctgcat gtcggcagcc tgggcttgta agctcttctt gcaaacttca
      721 gggaagtgtc cggcttcatt gaccatgccg cccttcatgc cgcccatgcc aagtgcgcag
      781 gtcttgcagg cgtttttgga gcgcatgcgc tgccatagct tgagcggccc tacttccagg
      841 gcttttttca gggtgtaaat gacggcttgc aagccgccgc cgctatctac tttcagtcgc
      901 atctagtacc caccggtaaa gccgaaccag taaaccggac cagtaaatat gattataagg
      961 tggcgcaatg atgtaaggcg gaacaaacca tttagaatat tcatcttata aagactcggc
     1021 agtcctggct gttgtcatga taacactcgg gaccttcgga actaattgga ggaatttatg
     1081 aagcgtaagc gcgtggcaac catggcaaag aggttggctg tattttccat gcttagcacc
     1141 ctctatccgc tggggcttcc agccggtgct gccgatgtga atagtttgct tgcctcggtt
     1201 tcttccatct ccgatgtgga tgccagacaa gccagtatcg aaaaacgttt gcaagatgcc
     1261 tacaatgccg ggcgcctcac ccaggcccag tacgagggct ttaagcaagc cctggcccgg
     1321 gttgccgacc aggaagcggt ctttcgcgct tccgacagca gcctcagcct ctgggaaagt
     1381 ttgcgattgc agtttgatat tgatcgcatc gtcaaagaaa tggaagccag tctcgccgat
     1441 aggcaggtgg gcttcgtcga tgttttcgcc cgccgcgatg aaatcaccaa acggcttggt
     1501 gtggcttatt ccgatggtcg tttgacctca caggaatatg ctcagattca gatggagctg
     1561 gaaaacatca acaaaaccat ccaggtccag agtgcagcta acggacattt gagcgtagct
     1621 gattccatca agttggtact ggaactagac cggctcagcc agaagctcac ccgcacggtg
     1681 cacgatcgtc aggtcagttt gacggctatt gatgtgcgtc aatctgagat cgagaagaaa
     1741 attgaaggcg gcgtggctag cggcaaacta accgatagag aagctgccga tctgagaggc
     1801 gagttcaagc gcatcgccga aagagaagaa agtctgcgta aattgggccg ccccctcagt
     1861 gctgaagagc agctaactct tgctcttgac cttgagaagc tgaacggcca ggttgatgca
     1921 caggtgtttg acaatgaggt gcaagacaac agcgccatca ccatggacaa gcgccgcacc
     1981 gatgtagata aaaacctggc cgccgccctt tcagccggtc tgattacatt gagcgaagcg
     2041 caagtgtata aggacgaact ggatgctgtc gttaagaagg aagagagctt caagaccgct
     2101 tccggtggac agttgaccct ggtgcaagcg cagaatctgc ttatagatct tgagcgcttg
     2161 aacggcaaaa tcgaccgtgc cgtcttcggt agaaaaccag tatgggtagg catcgaaggt
     2221 agtatcgatg atattcgtcg tcgtattgcc gatgctaaag tgggacaacg cctggccgaa
     2281 gttgatgaag ttgaactgaa gagcgatctc gataaagtga ttgccaacaa agcaacctat
     2341 gctgcttctt ctgccggtat cgacagccag gaggcaatca gcctggccgt tgatctaacc
     2401 aagctgaacg gcaagatgtt caaactgatc aaagatcgcg aaatttccgt actgccagat
     2461 ctggttaagc gcaaagacga aatcaataag cgcatgtccg acggcacatt aagcggcaag
     2521 ctgaccagtg atgaaacccg ttctctgctt gaagattttg atagaatttc agccagggaa
     2581 gcggccttcc gggccaccga cggtattctg gatgagcggg aaaaacttag cctggctctt
     2641 gatcttgagc gcctggctgc ccgtctggaa aaagaaattc gcgataatcc gtatgtgtcc
     2701 ttgcctctca gtcagaaaaa gactcaagcc gatcagatta tttccaccgg tacgctgacc
     2761 ggcaagttat ctgctcaaga agtgcagaat ttgagagccg aatacaatcg catcacggct
     2821 ttcgaagccg ccagtctggc atcgggctcc acccacgatg ccaaagagg
//
LOCUS       DAOQNZ010000410         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18801_length_2867_cov_5.005334, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000410 DAOQNZ010000000
VERSION     DAOQNZ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18801_length_2867_cov_5.005334"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PKW73_08350"
     CDS             <1..1343
                     /locus_tag="PKW73_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HNA73334.1"
                     /translation="RERFAEAEPFFDKAVGILRKCGCDVSHLPAFCEASGVSVYSGAA
                     DCKAKLGKIEEACELIQIAQEVAFATLPENHPLRMQTVLEAGVLQFAAKRKTTGGADA
                     GNLAHQSLLAGAEDDLVKALTALSQAMSESSGADSASACVESSAKGSKGAPKGTPKGT
                     PKGTPSNVVPLFSLGSKPIAITFGPDDLDEACDDDDDDELIDEDASAYQLKVTLKSVS
                     PPVWRRVVVPSSFTLFDLHNVIQRAMGWSRCHLHEFEVFGTTYSDGECRETFASSFFD
                     EDEDESAVCLARLGLTEGSKFLYRYDFGDGWEHSIQVEKVFPLDADIEEVQFVKAKGP
                     CPPEDCGGPHFYSHIMRVIKENAIKRKDLPWWVEDFDPTSVPIMFQTEDAGGSAKKKA
                     SSRKNKVEGVSAKGSSKNGASQKDISEKGVSGAHTRASAKRAVRQKSLSEPGAK"
     gene            1652..2212
                     /locus_tag="PKW73_08355"
     CDS             1652..2212
                     /locus_tag="PKW73_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73335.1"
                     /translation="MRKMHPLRVWLDVDNKYLFRFRTAAGDKAFEFSVVNNGSAIEGI
                     SAEFSLLADNDMAERCIYGCILALNHARVAGGVRFGQLMEPCEPVAIEFLGKSAAETS
                     KYVVDLRDPNGAYFREEVLVSHKEPAKQVLWSGGQCSFGYDQFARLAVLEPKPSHYKI
                     IDAILQFDNSRYMISYPDESPMEIDL"
     gene            2321..2770
                     /locus_tag="PKW73_08360"
     CDS             2321..2770
                     /locus_tag="PKW73_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73336.1"
                     /translation="MTGVPKETRDPQIIIVREKADGADYKLIAIVALLLASPVILFFG
                     LPFMAVAFVTMESWLPFFLVGLLAVSLVPVLRERLNNQVLVKDLEGRIKKLEAELSEA
                     RHALILMDEANEFHSKLQLEAKKQPEQNAQSSAPSESNDAGCTDLHS"
BASE COUNT          691 a          629 c          789 g          758 t
ORIGIN      
        1 atcgcgagcg gtttgccgag gctgagccct tcttcgacaa ggctgttggt attttgcgca
       61 aatgtggttg tgacgtcagt cacctgcctg ccttttgtga ggcttccgga gttagtgttt
      121 attctggtgc cgctgactgc aaggctaagc ttggcaaaat cgaagaggct tgcgaactca
      181 tccagattgc tcaggaggtg gcgtttgcca ccttgcctga gaaccatccg ctcagaatgc
      241 agactgtttt agaggccggt gtgcttcaat ttgccgcaaa gagaaagacc actggcggcg
      301 ctgatgccgg caacctggcc catcagtctt tgcttgccgg cgctgaagat gatttggtta
      361 aggctttgac ggctctttcg caagccatgt ctgaatcttc cggtgctgat tcagcttcag
      421 cctgcgttga atcctcggct aagggaagca agggcgcacc aaagggaacg ccaaagggaa
      481 ccccaaaggg aacgccatcc aatgtggtgc cactctttag tttgggttca aaacccattg
      541 ccatcacatt tggtcctgat gatcttgacg aggcttgtga cgatgatgat gatgatgagt
      601 tgatcgatga agatgcctct gcctatcagt taaaggttac tttaaagtcg gttagcccgc
      661 ctgtctggcg cagagttgtg gtgccttcca gctttactct ctttgatttg cacaacgtaa
      721 ttcaaagagc aatggggtgg agtcgttgcc acctgcatga gtttgaggta tttggcacta
      781 cctatagcga cggcgaatgt cgcgagactt ttgcaagttc cttctttgac gaagacgagg
      841 atgaaagcgc agtctgcctg gcgagattgg gacttactga aggttcgaag ttcctctatc
      901 gctacgactt tggcgatggc tgggaacatt caattcaggt agagaaagtc tttccgttag
      961 atgcagacat tgaggaagtg caatttgtaa aagcaaaagg accctgcccg cccgaagatt
     1021 gtggcggccc tcacttctat tcacacatta tgcgggtgat taaagaaaac gccataaaga
     1081 ggaaagatct gccctggtgg gttgaagatt ttgatccaac ctctgtgccc attatgttcc
     1141 aaaccgagga tgccggcggg agcgctaaga aaaaggcgtc ctcaaggaag aacaaggtag
     1201 aaggagtttc tgcaaaaggc tccagcaaga atggtgcttc gcagaaggat atttctgaga
     1261 aaggtgtttc aggagcgcat acccgtgcct ctgctaaaag ggcggtcagg cagaagtcgc
     1321 tctctgagcc aggcgccaag tgactcacca gcatcaatcc gattttgtaa ggtgcgccgc
     1381 tctttgcttg tgtaatcgat cagttcatca gtgcgcactc cgggtgggtc gctgagataa
     1441 agcttttggg gcacgatgct cacgttgtgt ttgtagatgg atttgactct actggtaatc
     1501 taaggataag agaccctggc gaaggtgtga ggtatgagat gagcgtcagg gatttcgttc
     1561 aatattggaa tgggaattat gtctgcaaat agggtagcgt ttcacagaat gtaagcgacg
     1621 cgggaaaatt ggtcttggag cttggtacta gatgagaaaa atgcatccct tgagagtttg
     1681 gcttgacgtc gataataagt acttgtttcg ttttcgcact gccgccgggg acaaagcttt
     1741 tgagttttcc gttgtaaata acggctctgc aattgaagga attagtgcgg aatttagttt
     1801 gctcgctgat aacgacatgg ccgagcgttg tatttatggt tgcatcttag ccttgaacca
     1861 tgccagagtt gccggtgggg tgagattcgg acaattgatg gagccgtgcg aaccagttgc
     1921 aatcgagttt ctgggaaaat ccgctgctga gacatcaaaa tatgtagttg acctcagaga
     1981 tccaaatggc gcctacttcc gggaggaagt cttggtgtcg cacaaagaac cagccaagca
     2041 agtgctctgg tccggcgggc agtgttcttt cggttatgat cagtttgcga ggctggctgt
     2101 tttggagcct aagccttcgc attacaagat aatcgacgcc attcttcagt ttgataattc
     2161 caggtatatg atcagttatc ctgatgagag tcctatggaa atagacttgt agcgtaacct
     2221 catgtcttag tgtccaaccg gtggctctaa atgcgctaaa atttgcatag cagtctgtga
     2281 atctaatgag acggatgttt gcatggacca ggagccaaaa ttgaccggag taccgaaaga
     2341 gacgcgagac ccgcagatta tcattgtccg cgagaaggct gatggtgccg attataagct
     2401 catcgccata gtcgccctgc tgcttgccag tccggttatt ttgttcttcg ggctaccctt
     2461 tatggccgtg gcttttgtaa cgatggagtc ctggttgccg tttttcctgg tgggtctact
     2521 tgccgtcagc ctggtgccgg tattgcgaga gcggctcaac aatcaagtgc tggtcaaaga
     2581 tctagagggg cgaattaaga agttagaggc agagctttca gaagcccgtc atgcacttat
     2641 cttgatggac gaagccaatg agtttcacag taagctgcag cttgaagcaa agaaacaacc
     2701 tgagcaaaat gctcagtctt cggcgccgtc tgagtcaaat gatgcaggct gcaccgactt
     2761 gcattcataa acgaatgttt tgatgccttt cgactttgag cgaaattccg tcgaatttcg
     2821 ctcacgcctc ggtttttcca aaggcacact gccgaccaac ggttagt
//
LOCUS       DAOQNZ010000411         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18860_length_2862_cov_4.771286, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000411 DAOQNZ010000000
VERSION     DAOQNZ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18860_length_2862_cov_4.771286"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKW73_08365"
     CDS             complement(<1..685)
                     /locus_tag="PKW73_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNA73337.1"
                     /translation="MFVRQLIVVSGYYGFDNLGDEAILEELVRELKSLWPAKDIVILS
                     QNPESTAARFGVRAQNRWKLSEIAALLRHCKLFISGGGGLFQDSESVKSVIYYGGLIS
                     LATLCGAPTLIYAQGLGPLKSALGKALTRFSLVQPKQISVRDETSLNLLKEWGLANVS
                     QRTGDPVWLLPRAELEQDLMEPIENCKRSGDKLVGLSLRDGGGFSLRHIESLAIAMHK
                     QLSPQYRLMP"
     gene            766..2106
                     /locus_tag="PKW73_08370"
     CDS             766..2106
                     /locus_tag="PKW73_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNA73338.1"
                     /translation="MGLEDICNVMERGSAASFAGADTDVNWFLVQPASVDELANLFHI
                     FSEAALVPYYDPKLKSLLATDTVFVDMKKLSAVTENVVSDLVMGVQCGITVFELRRVL
                     SAQKQIFPVSRVYDDLSLAQLMALGDSGACESGYGQLRSNVLGFEFLDTQGHRTKCGG
                     RVVKNVTGYDVSKLFVGSGRLALPYLVYLRLFARAEAAVLFLAQGPVLELLHFSSRLL
                     ASGINPYILELVQGEHGEFSLYIGLDGPTPMVEELKGLLAKLASAEGLLLKQCPGEDV
                     EEVSFEAAALERLEVFLSLEGLAFVLRHLSLIPAGIGLGAEVDSWSVRVRPATGRLII
                     DCSSYSQETNWLAAMEAAEYYLTEALRLWKQEIETFSPARREFFRWENFSIMKRVAGR
                     LIDTTGGETLASPPQKMGASIVGSLEHGLDPRRRFHPFVWAEKSRLSRVGAIEP"
     gene            2103..>2862
                     /locus_tag="PKW73_08375"
     CDS             2103..>2862
                     /locus_tag="PKW73_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNA73339.1"
                     /translation="MKEASDCKLEGACDGPINLFNLQVAPDLLSACIHCGLCLPACPT
                     YLATGHESQSPRGRIYAVMKSQGGQVERALDHLSTCLGCLGCQTACPSGVQYESILEQ
                     VRPALAARRSWPARTAMRLFFRLLGNYGLLRLLFIPVRFVQRLSLDRLPEVLVRLCPF
                     LKGTWLGRLASWRQFTPAVPVHKSLPARLAGQGAEKPVPVQFFQGCVMDIFYNQVNHA
                     AMKALAGAGLTCNLPRQTCCGALAFHAGDVDIARQ"
BASE COUNT          556 a          723 c          833 g          750 t
ORIGIN      
        1 ggggcatcag tctatattgc ggtgaaagct gcttgtgcat ggctatagcc aggctttcta
       61 tatgtcgtaa gctgaagccg ccgccgtctc tcagcgaaag tcccacaagc ttgtcaccgc
      121 ttctcttaca gttctcaatg ggctccatca ggtcttgttc aagctcggcc ctggggagca
      181 gccagacggg atcgccggtg cgctgtgaaa catttgccag cccccattct ttcaggaggt
      241 ttagtgaagt ctcatcacgc accgagattt gctttggttg taccagtgag aagcgggtca
      301 gggcttttcc cagagcgctt ttgagaggac caagaccctg ggcatagatg agtgtggggg
      361 cgccgcagag ggtggccagg gaaataagtc cgccataata aataaccgac ttgactgatt
      421 cactgtcctg gaatagtccg ccgccgccgc ttataaacag tttacagtgc ctgagcaagg
      481 ccgctatttc agatagcttc cagcggtttt gtgctctcac gccaaagcgg gccgcggtgc
      541 tctcaggatt ttgggaaagt atgactatat cctttgccgg ccaaaggctt ttcagttccc
      601 gcaccagttc ttccaagatg gcttcatcgc ctaaattgtc gaacccgtaa tatcctgaga
      661 cgacgattag ttgccgcaca aacactgcca cttctagctt tacttcaggt tgattctagc
      721 agttttcggc taatattagt gctagtgcct gagtggaaat atcttatggg tcttgaagat
      781 atatgcaatg tgatggagcg cggttcggct gcgtcctttg ccggcgccga cactgatgtc
      841 aactggttcc tggtgcagcc ggcatctgtg gatgagctgg ctaatctctt ccacatattc
      901 agtgaggcgg ctctggtgcc ttactacgac ccgaagttga agagcctgct tgccactgac
      961 acagtctttg ttgatatgaa aaagctctcg gccgtaactg agaatgtggt atcggatttg
     1021 gttatgggcg tgcagtgcgg aattacggtt tttgaactga ggcgagttct ttcagcgcaa
     1081 aagcagatct ttccggtatc aagggtttac gacgatctta gtctcgctca actaatggca
     1141 ctgggagact ctggggcttg tgagtctggt tacgggcagt tgcgctccaa tgtcctcggt
     1201 ttcgaatttc tagatactca agggcaccgc actaaatgtg gtggcagggt ggtgaagaac
     1261 gtcaccggct atgacgtaag caaattgttt gtgggctccg gtcggctggc actgccttat
     1321 ctggtttacc tgcggctttt cgccagagcg gaagctgccg ttttattcct ggcccagggc
     1381 ccggtgctgg agcttttgca tttttcctcc aggcttttgg caagcggtat caatccttac
     1441 attttggagt tggtgcaggg tgagcacgga gaattcagcc tctatatcgg attggatgga
     1501 ccgacaccga tggtggaaga actgaaggga ttgctcgcaa agttggcttc agccgagggc
     1561 ttgctcctta aacagtgccc aggtgaagat gtggaagagg tgagcttcga ggctgccgct
     1621 ctggagcgcc tggaggtctt tctttccctg gaggggctgg ctttcgtgct tcgccatctg
     1681 agcctcattc cagcgggaat aggactggga gccgaggttg atagctggtc ggtgcgggtg
     1741 agaccggcca cgggtagact tatcattgat tgtagctctt actcgcaaga gacgaattgg
     1801 cttgccgcca tggaagcagc ggagtattat ttgactgagg ctttgaggct ctggaagcaa
     1861 gaaatagaaa ctttttctcc ggccaggcgc gagttctttc ggtgggagaa tttttccatt
     1921 atgaagcgag tggctggtcg cctcattgat actactggtg gtgaaactct tgcctcgcct
     1981 cctcagaaaa tgggggcgtc cattgtcggt agtctggaac acggacttga tccccggcga
     2041 cgcttccatc cttttgtctg ggcggaaaag tctcgcctta gccgggtggg agccatcgaa
     2101 ccatgaagga agcctccgac tgcaaattgg aaggcgcttg cgacgggccg attaacttgt
     2161 ttaatttgca agtggcaccg gatctactgt ccgcctgcat tcattgtggc ttgtgtctgc
     2221 cggcctgtcc tacctatctg gccaccggac acgaaagtca atcgccccgg ggcagaatct
     2281 atgccgtaat gaagagccag ggcgggcagg tagagcgagc cctcgatcat ctttctacat
     2341 gcctgggctg tctgggctgt cagactgctt gcccctcagg ggtgcaatac gagtcgatac
     2401 tggagcaggt gcggccggct ctggccgcca ggcgcagttg gcctgcccgc acagccatgc
     2461 gccttttctt tcgcctactg ggtaattacg ggctcctgcg cttgctcttc ataccggtgc
     2521 gctttgttca gcgtctttct ttggatcgcc tgccggaagt attggtgagg ctttgtcctt
     2581 tcctcaaggg tacctggctt ggtcgccttg cttcctggcg gcagtttact ccggcagtac
     2641 cggttcacaa gagtttgcct gccaggctgg ccgggcaggg ggcagaaaag cctgtgccgg
     2701 tgcagttctt tcagggctgc gtcatggaca ttttctataa ccaggttaat catgcggcga
     2761 tgaaagccct ggctggcgcc ggtctcactt gtaatctgcc ccggcagact tgctgcggtg
     2821 ccctggcctt ccatgccggc gatgttgata ttgcccgcca gt
//
LOCUS       DAOQNZ010000412         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18864_length_2862_cov_3.982187, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000412 DAOQNZ010000000
VERSION     DAOQNZ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18864_length_2862_cov_3.982187"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..793)
                     /locus_tag="PKW73_08380"
     CDS             complement(89..793)
                     /locus_tag="PKW73_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73340.1"
                     /translation="MESAAGGLYEAWMSREAIRFKSHSFGYTLYARSPDWQVTACRDD
                     CKEKTSTSLKEFWREREGVMGACSVLALQKPLKVNTRQFPFLSFPGIAPRGLKGKEFV
                     FAGEQRANDLFVTAERPKETAAYVTLTADLGLPAEVYSLVSVSLNMPRLDGCLLETSE
                     YFKDGERCWVIRRRGLAPVSVPPDFFEPPRNYKYAGKLKRQFLFQGVSGIMDDVAAGL
                     GPAKEKAAKGAVGAKH"
     gene            1089..>2862
                     /locus_tag="PKW73_08385"
     CDS             1089..>2862
                     /locus_tag="PKW73_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA73341.1"
                     /translation="MLPEDKSSELSRPEKPESISTKGQSAQVDAFRIKVPGKLRSGFD
                     ISQTAVEKPEAASRLAPGMLVDGKYRLLRPLGQGGMGVVWLVEHVMLKRELALKTLVH
                     SRMSQAVYERFAKEIRTTSQLDHPNLVKVFDCGLLPDGTPYYTMEYAAGHSLRQYLDE
                     KGPMPLKQCLEVFVDVAGALSYAHRHNTLHRDIKPDNIVLVRNGDGFRAQVLDFGLAK
                     FVHSGLKPITESTAGDYIGSPPYMSPEQCMGKDLDYRSDVYSLGCTLFQALTGVLPFE
                     SKNHLAIIQMHLSERPPSLQQAANGEQFPEPIEIIVSRMLAKDPKQRCRSMEEVERLL
                     KLYLRGKFTAGNRSGTEQFDADYADAQSHTSGKSDYSGPLLIAAILGIILLGGAGVTY
                     FLLQPPAAKVAEVKPDPKLKFATRLSSPPVAGEQFRRFHFPIEISSGKFEAVNSDFKR
                     EARGETEIPVNALVIFEPSREFLKDPAVLTRFRPDDLVGLVLNKHNKADDDTLLACTA
                     LDGLKHLSITSPKVTDKGLAYLASLNELKWLDVGDADISGKQLSLLPCLPNLNYLRLD
                     TDRSSPDFSDTFKALHGSKNLYTLS"
BASE COUNT          695 a          837 c          711 g          619 t
ORIGIN      
        1 gcatcctcct ttcatacagt ttcatggcgg cggtcagtta gccgttgaga caggcttgat
       61 tatatccagc cgacctgagc agtgcttgct aatgttttgc accgacggca ccttttgccg
      121 ctttctcctt ggccgggccg agccctgccg ccacatcgtc catgatgccg ctcacaccct
      181 gaaagagaaa ctgtcgcttc agtttgccgg catacttgta attgcgcggc ggctcgaaaa
      241 aatcaggcgg aaccgagacc ggcgccaaac ctcgccttct aatcacccag caacgctcgc
      301 cgtctttgaa gtattcgctg gtttcaagca agcagccatc caggcggggc atgttcaaag
      361 ataccgagac caggctgtaa acctccgccg gcagtcccag atcggcagtg agagtgacat
      421 aggccgccgt ttctttcggg cgctcggcgg tgacaaagag atcgttggct ctctgttcac
      481 cagcaaaaac aaattctttg cccttcagcc ccctgggagc aataccggga aaggagagga
      541 agggaaactg cctggtattc actttcaaag gcttctgcaa ggcaagcaca ctgcaagcgc
      601 ccattactcc ttcccgttct cgccagaact ccttcaaaga ggtgctggtc ttctccttgc
      661 aatcgtcgcg gcaagctgtg acctgccagt ccggacttct ggcatataac gtgtaaccaa
      721 aagaatggct tttgaaacga atggcttctc ggctcatcca ggcttcatag agtccgcctg
      781 cagccgactc caactgtaag cgccagactc taggtgccga cgctgccggc ccgggggctg
      841 ctataactgg taacaaagcc agaccgaccg cgaagctaca acagagcaac gccagaccga
      901 ttgcaaacct tctagaactc gggaattgcc tcattaaacc tcattgcctg aaactgaacc
      961 gatccgcatt ttcacatctg caattctatc ccctcaatca atgccgggtg ttaagcccag
     1021 aatccgccac acatagtgtg cataataaat cgataccccc cttgccccaa ttttcttttc
     1081 ttagagccat gttgccagaa gataaaagct ccgagctcag cagaccggaa aagccggaaa
     1141 gtatttcgac aaagggccag tcggcccagg tcgatgcctt ccgcattaag gtacctggca
     1201 agttgcgctc cggtttcgac atttcccaga ccgccgtgga aaagccggaa gcagccagtc
     1261 gactggcacc gggcatgctg gtggacggca agtatcgcct gctccgtcct ctcggccagg
     1321 gcggcatggg tgtggtctgg ctggtagagc atgtaatgct caagcgggaa ctggcgctca
     1381 aaaccctggt tcattccaga atgtcccagg ccgtctacga acgtttcgcc aaagaaattc
     1441 gcaccacctc ccaattggat catcccaatc tagtaaaagt attcgactgc ggtcttttac
     1501 cggatggcac gccttattac accatggaat acgccgccgg acactccctc aggcaatacc
     1561 tggacgagaa gggacccatg cctctcaagc aatgtctgga agtgttcgtg gacgtggccg
     1621 gcgctctcag ctatgcccac cgccacaata ccttgcaccg ggatatcaaa ccggacaaca
     1681 tcgtgctggt caggaacgga gacggcttcc gagcacaagt tctggacttt ggtctggcta
     1741 agtttgtgca cagcgggctc aaacctatca cggaaagcac ggccggcgat tacataggca
     1801 gtccgcccta catgagcccc gaacagtgca tgggcaaaga tctcgactac cgctccgatg
     1861 tttattctct tggctgtact ctctttcagg cactcacggg tgtgctgccc ttcgaatcaa
     1921 agaatcatct ggccattatt cagatgcact tgagtgaaag gccgccctct ctgcagcagg
     1981 ccgctaacgg cgagcagttt cctgagccta tagaaataat cgtcagccga atgctggcta
     2041 aagatcccaa acagcgctgc cgctccatgg aagaagtgga gcgcctcctg aagctctatt
     2101 tgagaggcaa atttacagcc ggtaaccgtt ccggcacaga gcagttcgac gccgactacg
     2161 ccgatgcgca aagccataca tcaggcaagt ccgactatag cggaccgctt ctcatagcag
     2221 caattctggg catcatactg cttggcgggg caggtgtgac ctattttctt ctgcaacccc
     2281 ccgcagcaaa ggttgctgag gtcaagccag atccaaagct aaagttcgct accaggctca
     2341 gttcgccgcc agtggcagga gaacagttca gacgatttca ctttcccatt gagataagct
     2401 ccggcaaatt cgaagccgta aattcagatt ttaaaagaga ggcccggggt gaaacggaga
     2461 taccggtaaa cgccctggtg atttttgaac caagccggga gtttctgaaa gatccagccg
     2521 tgctgacccg cttccgtccg gatgacctgg tgggactcgt gctcaataag cacaacaaag
     2581 cagacgatga cacactttta gcatgcacgg ctcttgacgg gttaaagcac ctgagcatta
     2641 ccagccccaa ggtcacggac aaaggtctgg cttatctagc cagcctcaac gaactaaaat
     2701 ggcttgacgt gggagacgcc gacatctccg gcaagcaact gagtcttttg ccttgcctac
     2761 caaatctcaa ttacctgcgg ctcgatactg acaggtcctc gccggacttt tccgatacct
     2821 tcaaagccct gcacggttcc aagaatcttt atacgctcag tg
//
LOCUS       DAOQNZ010000413         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18895_length_2860_cov_2.853119, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000413 DAOQNZ010000000
VERSION     DAOQNZ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18895_length_2860_cov_2.853119"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1641)
                     /locus_tag="PKW73_08390"
     CDS             complement(271..1641)
                     /locus_tag="PKW73_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMS domain-containing protein"
                     /protein_id="HNA73342.1"
                     /translation="MRIRKLHVLALSMSLSQTLMLQVPVFCQTSESAEDGSSLESMWS
                     EEAGKTDKNKKTESSKKSKSEAKAKMSDLGDAGAVTPADTSKPIPLDTSPAETARVES
                     PLCTIASFKESELVKTSCWPGIGPFQGQNEGRMEDPQENKLHYRQDGDKLTRAELLLV
                     GQNHDAQSLLNLQMATAYMLEALGAKNKRIAEFNAFLEKNKQHLSQKESQASISTTSG
                     PYAINLKNTTSDGAHAYLIEIHKRGEEAVGESLIALSSAGESRHIETEPEKEPQAPAR
                     VKPAPGKTLSTPVKLPPTKTATAKPPTTPVKAAPLEEVRKPETAGQDSLKQELQETLR
                     SWQNHKKDALKERQTEELSKYLSGRALGRQKDGIKWLVDNKKYYDMVPRGVTVDKYQQ
                     LSAAPPRYLVTARVKELSKLVDEATKQVMKESEDSYNVNYTLEKSGNTWTISDSQLIA
                     GAKGKK"
     gene            complement(1740..>2860)
                     /locus_tag="PKW73_08395"
     CDS             complement(1740..>2860)
                     /locus_tag="PKW73_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA73343.1"
                     /translation="LQTSGALKGKTIAWHCHLTLLTELAARAVVEAGARLVLSECNPA
                     TTQMEAVENLEKMGRVFLGQDSAVQALASKPDILADTGFVLIPTYLSSLPPETGADIM
                     GASEITTSGISKLRAYRGSFGFPIFNINDGQIKSLIENFHGVGDGLLEALKLHDALVP
                     QTRVAVVGYGKVGAGCAHYLKANGARVAVVEIDPVAGLVAHFDGYELATLEQALASSH
                     ILVTATGKAGLLGQEHFLQAADGLLIANVGHLNSEVSPQVLESLCEHKVEKSNGLTEY
                     HLKVPSPTGEQCGSRRLALLTGGNPCNVALLTGSDAPTMLHLTTEILTWDYIASQCRP
                     EKEKQLPSGITGERSLPPDVETLAARIALDALGYQFYA"
BASE COUNT          532 a          752 c          794 g          782 t
ORIGIN      
        1 tcctttggct cttccgcctt tttggcgagc aggagttccg gcttgctctt agcctcagcg
       61 cctacgaggg ggcagctcag tgagaggcag agggacatca gtgcgagcac cagggatagc
      121 ttcttgtttc cgggctttgc ggctctgtcc aggttcattt ctttcctctt tcactgttgc
      181 caagaaatat gttttcattc aacaagagga ttttactaaa aaagccgctg cagcgcatag
      241 aatcaggctt tccaagcggc agaaagctgt ttatttcttg cctttggcgc cggcaatgag
      301 ctgcgaatca ctgatagtcc aggtattacc tgatttttcc agggtgtaat tgacgttgta
      361 ggagtcttcc gattctttca tgacctgctt ggtggcttcg tccaccagct tgctcagttc
      421 cttgacgcgg gctgttacca ggtagcgggg aggggcggct gaaagctgct ggtacttatc
      481 tacggtgacg cccctgggta ccatgtcgta atacttcttg ttgtccacta gccatttgat
      541 gccgtctttc tgccttccca gggcgcggcc tgaaagatac ttgctcaact cttccgtctg
      601 cctctctttc aaggcatctt ttttatgatt ttgccaggag cgcagggtct cctgtaactc
      661 ctgcttgagg ctgtcctgac ctgccgtttc gggctttctt acttcttcca ggggagcggc
      721 ttttacagga gtagtcgggg gtttggcagt ggcggttttc gtcggcggca gtttcaccgg
      781 ggtgctcagg gtcttgcctg gcgctggctt taccctggca ggggcttgcg gttctttttc
      841 aggttcggtt tcaatgtgtc tgctttcacc ggccgaactg agggcgatga ggctctcgcc
      901 gacagcttct tcgccgcgct tgtgaatttc tatcaggtag gcatgagcgc cgtcgctggt
      961 agtatttttc aggttgatgg cataaggtcc gctggtggtg ctgatggaag cctggctttc
     1021 tttctgggaa aggtgctgtt tgttcttttc cagaaaagca ttgaactcgg cgatgcgctt
     1081 gttctttgcc ccgagggctt ccagcatata ggcggtggcc atttgcaggt tgagaaggct
     1141 ctgagcatca tggttctgac cgaccaaaag caattcggct cgggtcagtt tgtcgccatc
     1201 ctggcggtaa tgcaatttgt tttcctgggg atcttccatg cgcccttcat tctgcccttg
     1261 aaacggtccg atgccgggcc aacatgaagt tttaaccagt tcgctttctt tgaaactggc
     1321 gatagtacaa agaggggatt ctactctggc ggtctcagcc gggctcgtat caagaggaat
     1381 cggtttgctt gtgtcggctg gggttaccgc tccggcatcc cccaggtcgg acatcttggc
     1441 ttttgcttcc gacttagact tcttgctgct ttccgtcttt ttgtttttat cggtcttgcc
     1501 ggcctcttcc gaccacatag attcaaggga agagccgtct tccgcacttt cacttgtctg
     1561 gcagaagacc gggacttgaa gcatgagggt ctggctgagg ctcatactga gagccaacac
     1621 atgcagtttc cgtattctca tgatctcgtt tagctccatt taacggtctt gctccggaat
     1681 tcggcaaaat taccgcccca tgaggcgtca ctctgcagcc actgccggtg ccagtacatt
     1741 taagcataaa attgatatcc gagggcatct agtgcgattc ttgccgctaa agtctcaaca
     1801 tccggcggca gggagcgttc gcctgtgatt ccacttggca attgcttctc cttctctgga
     1861 cggcattggg aggcaatgta gtcccaggtg agaatctctg tggtcagatg caacatggtg
     1921 ggggcatccg agcctgtgag cagggctaca ttgcaggggt tgcccccggt gagaagtgca
     1981 aggcggcggc tgccgcattg ctcgccagtt ggcgacggca ctttcaaatg atattcagtg
     2041 agaccattac ttttttctac tttatgttca cagaggctct ccagcacttg cggtgacacc
     2101 tcactattga gatgcccgac attggcaatc aggagtccat cggcggcctg gagaaaatgc
     2161 tcttggccga ggagcccggc cttgcctgtg gcggtcacaa gaatgtggct gcttgccagg
     2221 gcttgttcca gggttgccaa ttcgtaacca tcgaaatggg ccacaaggcc ggccaccgga
     2281 tcgatttcca ccaccgccac ccgggcaccg tttgctttca agtagtgggc gcaaccggcg
     2341 ccgaccttgc cgtagcccac cacggctacc cgggtttgtg gcactagcgc atcgtgcaat
     2401 ttgagcgctt ccaaaagccc gtcgcccaca ccgtggaaat tttcaatgag agatttaatc
     2461 tgaccgtcat tgatattgaa gattggaaag ccgaagctgc ccctataagc tcttaactta
     2521 ctgataccgg atgtggtgat ttcgctggct cccattatgt cggcgccggt ctccggcggc
     2581 aagctgctca aataagtggg aatcaagaca aaaccggtgt ctgccagaat gtcaggcttt
     2641 gaggccagag cctggacggc gctgtcttgg cccaggaata cccggcccat tttctcaaga
     2701 ttctcaactg cttccatctg ggtggtggca gggttgcatt cgctcaaaac caagcgggcg
     2761 ccggcctcta caacagctct agctgccagt tccgtcagga gagtcaggtg gcagtgccag
     2821 gcaatagttt ttcccttgag ggcgccgctt gtctggaggg
//
LOCUS       DAOQNZ010000414         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18921_length_2858_cov_3.869782, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000414 DAOQNZ010000000
VERSION     DAOQNZ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18921_length_2858_cov_3.869782"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PKW73_08400"
     CDS             <1..1071
                     /locus_tag="PKW73_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA73344.1"
                     /translation="LDEPTNHLDLESIHWLEEFLANYEGTLIVISHDRHFLNAVTTHI
                     ADIDYDTIIVYTGNYDDMVSQKIQARQTIESANRDKAKKIAQLQEFVARFAAGQRSSQ
                     VQSRKKEMARLAPQELKKSNIQRPFIRFEQEKPSGKEVLIARNVSKGFDGNTLFEGLD
                     LEVLRGDKVAIIGANGVGKTTLLRTLIGELPPDDGTIKWTDSASWSYYPQDYHDEIKP
                     GLTALDWLMQFMVDEGQQYVRGILGRMLFSGDDSLKATEALSGGEAARLLMARMMMLK
                     NPVLIFDEPTNHLDLEAVSALAEGLAQFPGTVFVVTHDRDLVSNVATRILSFKDGKLI
                     NFAGTYEEYLQDHPLKELARKR"
     gene            1079..1879
                     /locus_tag="PKW73_08405"
     CDS             1079..1879
                     /locus_tag="PKW73_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73345.1"
                     /translation="MNPEETNPILSAPEALRLALEALEHPASEIAEKNFKIIVMALEA
                     RHGENQKEVAGELQKLSKEIEAMGSLDSAMEFKQRSTEMMLKLGMERRRARQSGSGMP
                     APAVVAQTQNTATGTASPIAPPRPQAPAPRRPGDSTATRLVRHTHSSNTGLFQRLIFM
                     VHTSTSYDSDLNLYTRTMGGKVDWALDSGGKRYRAVMFAQEPTILLVESDTLPRLLPI
                     FAVTDLDKARQELSERGLVEKETLISPLGTCHIFKANVGISLGIIHQE"
     gene            1886..>2858
                     /locus_tag="PKW73_08410"
     CDS             1886..>2858
                     /locus_tag="PKW73_08410"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HNA73346.1"
                     /translation="MSRNLFRAYCRKNGDATPPVVPAHTLLLAFEESGILHDDKRLSK
                     FFAALKKAPGELTESQFESLLEISPTLVEQVLTKQLAIPQFQDFCGHIADIFAEVEEN
                     RGGTLANYIPQLEKVDPEKFAVSVCTVDGQRFNLGDAEDYFCVQSCSKPITYCLALEA
                     RGEEEVHKYVGAEPSGKTFNELTLNAKSQPHNPMINAGAIMCGALLKSESLDASARFD
                     YVMKQWRECAGQEKVGFDNAVYLSERQTADRNFALAYFMREKKAFPPGVDLLDALEFY
                     FQCCSIEITTRAMSIVAATLANGGNCPLTGQQVFKPNHVKNCLSLMSS"
BASE COUNT          728 a          860 c          718 g          552 t
ORIGIN      
        1 ctggacgagc ccaccaacca cctggatctg gagtccattc actggctgga ggaatttctc
       61 gccaattacg aaggcaccct gatcgtgatt tctcacgacc gccacttcct caatgcagtc
      121 accacccata tcgccgacat cgactatgac accatcatcg tctacaccgg caactacgac
      181 gacatggtaa gccagaagat acaggcccgg caaaccatcg aatcagccaa ccgcgacaag
      241 gccaagaaaa tcgcccagct acaagaattc gtagcgcgct ttgcggccgg tcagcgctct
      301 tcccaggtac agtccaggaa gaaagaaatg gctcgcctgg ccccccagga acttaaaaaa
      361 tccaatattc aaagaccctt cattcgcttc gaacaggaaa agccttccgg caaagaagtg
      421 ctgatcgccc ggaatgtaag caaaggtttc gacggcaaca ccctcttcga aggtctggat
      481 ctggaagttc tcaggggcga taaagtggcc atcatcggcg ccaacggtgt cggtaagact
      541 acgctcttgc gcaccctgat tggagaatta cctcccgatg acggcaccat caagtggacc
      601 gactcagcca gttggagcta ctatccgcag gactaccacg atgaaatcaa gcccgggctc
      661 accgccctgg attggctcat gcagttcatg gtggacgaag ggcagcagta cgtgcggggc
      721 atcctcggac gcatgctctt ctcaggagac gacagcctca aggcaacgga agccctctcg
      781 gggggcgagg ccgcccgcct gctcatggcc cgcatgatga tgctcaagaa tccggttttg
      841 atcttcgacg aacccaccaa ccacctcgac ctggaggccg tgtcggccct ggccgaaggc
      901 ctggcacagt ttccgggtac agtctttgta gttacccatg accgcgacct ggtatcgaat
      961 gtggccacaa gaatactctc cttcaaagac ggcaaattga taaacttcgc cggcacttac
     1021 gaagaatact tgcaggatca tccactgaag gagctggcac gcaagcgcta gactataaat
     1081 gaatccagag gaaaccaatc ccattctcag tgcaccggag gccctcaggc tggcgctgga
     1141 agccctcgag catccagcca gcgagattgc cgagaagaac ttcaagataa tcgtcatggc
     1201 tctggaagct cgtcacggtg aaaatcagaa ggaagtggcc ggggaactgc aaaagctctc
     1261 caaagaaatc gaagccatgg gcagcctcga ctcggccatg gaattcaaac aacgttccac
     1321 cgagatgatg ctcaaactgg ggatggaaag gcgccgcgcc agacaatcag gctcgggcat
     1381 gcccgcaccg gcggtagtgg cgcagacgca gaataccgcc accggtaccg ccagtccaat
     1441 tgcaccgccc agaccccagg caccggcccc cagaagaccg ggagacagta cggccacaag
     1501 actggtgcgc catacccatt cgtcaaatac cgggctcttt caacgtctaa tttttatggt
     1561 gcatacttcc accagttatg acagcgatct caatctctac acccgcacca tgggcggtaa
     1621 ggtagactgg gcactggata gcggcggcaa acggtataga gccgtaatgt tcgcccagga
     1681 gccgactatt ctactggtgg aaagcgacac cctgcccaga ctgcttccca tttttgccgt
     1741 caccgatctg gacaaggcca ggcaggagtt gtccgaacgt ggactggttg aaaaggaaac
     1801 cctgatctcg cccctgggaa cctgccatat tttcaaggcc aatgtcggaa tcagtctggg
     1861 catcatccac caggaataaa tctttatgtc cagaaatctc tttcgcgcct attgccggaa
     1921 aaacggagat gccacaccac cggtagtgcc agcccacacc ctcttgctgg cctttgagga
     1981 gagcggtatt ctccatgacg acaagcgact ctcaaaattc ttcgccgccc tcaaaaaggc
     2041 tccaggggaa ctaaccgaaa gtcagttcga aagcctgctg gaaatttcgc ccaccctggt
     2101 ggaacaggtt ctcaccaagc aactggccat cccccaattt caagatttct gcggtcatat
     2161 cgcggacatc ttcgcggaag tggaagaaaa ccgcggcggc acgctggcca attatattcc
     2221 ccaactggaa aaggtcgacc cggaaaaatt tgccgtctcc gtctgcactg tagacgggca
     2281 gcgcttcaac ctcggtgacg ctgaagacta tttctgcgtg cagtcctgct ctaaacctat
     2341 tacctactgc ctggccctgg aagcccgcgg tgaagaagag gtgcataagt atgtcggcgc
     2401 cgaaccaagc ggcaagacct tcaatgaact gactttgaat gctaagagcc aaccccacaa
     2461 tcccatgatt aacgccggcg ccatcatgtg tggtgcgctg ctgaaaagtg aaagtctcga
     2521 tgcttccgct cgcttcgact atgtcatgaa acaatggcgc gaatgtgccg gacaagagaa
     2581 ggtgggcttc gacaacgccg tctacctctc ggaaagacag accgccgatc gcaacttcgc
     2641 ccttgcttac ttcatgcgcg agaagaaggc cttcccgccc ggcgtagact tattggatgc
     2701 cctggaattc tacttccagt gttgctccat tgaaatcacc accagagcca tgtctattgt
     2761 ggcagccacc ctggccaacg gcggcaactg ccccctcacc gggcaacagg tcttcaagcc
     2821 caaccatgta aaaaactgcc tctccttgat gtcctcct
//
LOCUS       DAOQNZ010000415         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18941_length_2857_cov_3.612063, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000415 DAOQNZ010000000
VERSION     DAOQNZ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18941_length_2857_cov_3.612063"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKW73_08415"
     CDS             <1..653
                     /locus_tag="PKW73_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73347.1"
                     /translation="AITKVLAIQPGAPETYVLAGQLRNELGEFSQAVEAFNRALALAP
                     GHVNALCGRAYSKDLLNDFAAAVVDFDSAIEYEPRNHFLWIDKGYTLLKWRRCKSALA
                     AFSVAVRLKPEIGAIYAARALAYEMTGDYDAAISDLNKSIELRPRSWFPYIYRAKFFL
                     RTRSYNLAMQDYLKAFSFNKRETLERMWEDVSQHLVRCKSTPARKTSTGSANSRKS"
     gene            complement(655..2172)
                     /locus_tag="PKW73_08420"
     CDS             complement(655..2172)
                     /locus_tag="PKW73_08420"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNA73348.1"
                     /translation="MADLESNAQSAPEGNTEHQGSCAPANGKHNDCEADASGIKLFVP
                     EGVRYNCQGCGRCCSGWSVGMTEEDYGRIKDIDWQSLHPELAGKELFFHREEEFKAGL
                     AGHPHYTKPRADGTCPFLINKLCFIHGHLGEDQKPVTCRLFPYSFVETPSGVYTGVVY
                     NSMAAAKNQGDLLTDQKDALLDYLALTRKYATALNKTAAAMEVKDKPKSLETGALVDA
                     PVESNVPFQTVELTLGTVVTWEEFLEVDNKLMDLMLNRKDLNIFQVLPAGSEILQKAI
                     RLKRAGSPMTELRDFDPVVASDADMTPGAVEEMTLRTMFYRFFIYPMIRVDEKGLWQM
                     QRRNILNPVNAFMVARSFSRYTFSALGAILFKHAKVPGAGNMNLEAAAKKHFEPLSKE
                     LDDYFKRWLYLKLFAKTYFGPAAAGFGVVSGYNCLMASIIAVMIFAKCCATSRKEKAL
                     NIDDIYEAYWRLDRELLTMGQVSKQESVAFNFAFATPRLFHKMLFELQQGFKGGS"
     gene            complement(2208..2447)
                     /locus_tag="PKW73_08425"
     CDS             complement(2208..2447)
                     /locus_tag="PKW73_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73349.1"
                     /translation="MPKIPLENQFIYLMCLVIFLGLVQQYQEGYFHRPLPSKAAIKDA
                     QAGNLAIRIFRQMDLARNMERLELERHLLLYGRVE"
     gene            complement(2459..>2857)
                     /locus_tag="PKW73_08430"
     CDS             complement(2459..>2857)
                     /locus_tag="PKW73_08430"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNA73350.1"
                     /translation="HLFFLQQSTLFGVLELKTWLVLSLFSWLYRAGLQASPMQATPGE
                     FLLGLKITTKEGKRLSLKQATLRHFARFLCPLSLGAAYFLPLINQQQKSIEDIVTGTK
                     VSSVDQPRLEEELDLLPSALKQQPFPYTSM"
BASE COUNT          628 a          659 c          825 g          745 t
ORIGIN      
        1 cggcaataac aaaagtgctg gccattcagc caggtgctcc ggagacttat gtgcttgcag
       61 gacaattgcg taatgagttg ggtgagtttt cacaagccgt agaagcgttc aatcgtgctc
      121 tggcgctggc gccagggcat gtgaatgctc tatgtgggcg agcttattca aaggacttgt
      181 taaatgactt cgctgcagcg gttgttgact ttgattccgc aatcgagtat gaacctcgga
      241 accactttct ctggattgat aaaggataca cactgcttaa atggaggcgt tgtaagagtg
      301 ccctggcggc ttttagtgtg gcggttagac taaagcccga gattggagct atctatgcgg
      361 caagagccct ggcctatgag atgactggtg actacgatgc ggccatttct gatctgaaca
      421 aatctattga gctgcgaccg cgatcatggt ttccatacat atacagagcc aagttttttc
      481 ttagaacccg cagctacaat cttgccatgc aagattatct gaaggcattt agctttaaca
      541 aacgtgagac cttagaacgg atgtgggaag atgtttctca gcatttagtc agatgcaaat
      601 ctacgccggc gcgcaagacc agcactggtt cagctaattc ccgcaaaagc tagcttaact
      661 accgcccttg aagccctgct gcaattcgaa gagcattttg tggaagagcc tgggagtggc
      721 gaaagcgaag ttgaaggcga cgctctcttg cttggatact tgtcccatgg ttaagagctc
      781 gcggtccaag cgccagtagg cttcgtagat gtcatcgatg ttgagcgctt tttcctttct
      841 ggaggtggcg cagcatttgg cgaagatcat gacggcaatt atggatgcca tcaagcagtt
      901 gtagcctgaa acgacgccga aaccggcggc agccggacca aagtaggtct ttgcgaagag
      961 tttgaggtag agccagcgct tgaagtaatc atccagttct ttgctgagag gctcgaaatg
     1021 cttcttggcc gctgcttcca ggttcatgtt gccggcgccc ggcactttgg catgcttgaa
     1081 gagaatggct cccagtgctg agaaggtata gcggctgaac gagcgcgcca ccatgaaggc
     1141 gttgacaggg ttgagaatat tgcgtcgctg catctgccag agacctttct catccaccct
     1201 gatcatgggg tagatgaaga aacggtagaa cattgtacgc agggtcattt cttccaccgc
     1261 ccctggtgtc atatcggcat cggaggccac caccgggtca aagtctctca gctcggtcat
     1321 ggggctgccg gcgcgtttga gacggatggc tttttgcaga atctcagaac cagccggcaa
     1381 aacctggaaa atgttcaagt ctttgcggtt gagcatgagg tccatgagct tgttgtcgac
     1441 ttccagaaat tcttcccagg tgactaccgt tcccagagtc agctcgacag tctggaacgg
     1501 cacattactt tccacgggcg catcaaccag agcgccagtt tcaagagact tgggtttgtc
     1561 cttcacttcc atggctgccg ccgtcttgtt aagggcggtg gcgtattttc tggtcagggc
     1621 caggtagtcg agcagagcgt ctttctggtc ggtgagcagg tctccctggt ttttggcggc
     1681 ggccatactg ttatagacca cgcctgtgta aacgccgctt ggtgtttcaa caaatgagta
     1741 ggggaaaagt ctgcaggtga cgggcttctg gtcttcgccc aggtgaccgt gaatgaaaca
     1801 caatttgttt atgaggaagg ggcaggtgcc gtctgctctt ggtttggtgt agtgggggtg
     1861 tccggccaga cctgctttga attcctcctc gcggtggaag aaaagctctt tgccggcaag
     1921 ttccgggtgg agggattgcc agtcgatgtc tttgatgcga ccgtagtctt cctcggtcat
     1981 gcccaccgac cagcctgagc aacagcgtcc gcaaccttga cagttgtacc ttacgccttc
     2041 ggggacgaag agcttgatgc cggaggcgtc tgcttcgcaa tcgttatgtt taccgtttgc
     2101 cggtgcgcaa ctgccttgat gctcggtgtt tccttccggt gccgactgag cgtttgattc
     2161 caggtccgcc attatttact ctcatgacaa ttatgtgcct acagagctta ttctaccctg
     2221 ccatacaaga gtaaatgacg ttccagctcc agcctctcca tgttgcgggc caggtccatt
     2281 tggcggaaga tgcgaatggc tagattgccg gcctgggcgt cttttatggc tgctttcgag
     2341 ggtaggggcc ggtgaaagta gccttcctga tactgctgga cgaggccaag aaatatgacc
     2401 aggcacatga ggtagataaa ttggttttcc aggggtattt tgggcatttt tactgacttt
     2461 acattgatgt gtaggggaag ggctgttgtt tgagggcgga aggcagcagg tccagttcct
     2521 cttccaatct tggctggtct acggaggaga cttttgtgcc tgtaactatg tcttcaattg
     2581 acttttgctg ttgatttatt agaggaagaa agtaggcggc tcccaggcta agggggcaaa
     2641 gaaagcgggc aaagtgtctg agggtggctt gtttgaggct cagccttttg ccttctttgg
     2701 tggttatctt tagtcccaag aggaattctc cgggtgtggc ttgcattggt gaggcttgta
     2761 aaccggcgcg atatagccaa ctgaagaggg agagaaccag ccaggttttc agttccagga
     2821 cgccgaagag agtggattgt tgaagaaaga acaagtg
//
LOCUS       DAOQNZ010000416         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_18967_length_2855_cov_3.061786, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000416 DAOQNZ010000000
VERSION     DAOQNZ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18967_length_2855_cov_3.061786"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..737
                     /locus_tag="PKW73_08435"
     CDS             84..737
                     /locus_tag="PKW73_08435"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNA73351.1"
                     /translation="MRVCHAGSCSLLAICLLGAAITAQAAPLDKPPPVPKHGATGRPV
                     EPVDSAPKAKDENNPAFHKALLEIAGQYTGYQLSDDLLRRAPERCALPVQELSQSADG
                     GSHGKKFYYLYVKDLAPYLKHDKVKPGQALVKESYKAIPTKTGPSQKVEYKVGDKYGL
                     FIMLKMPPQTVGTDEGWIYGTVTPDGKTVTGSGRMASCMSCHLSAPHGRLFGVKAAR"
     gene            786..1238
                     /locus_tag="PKW73_08440"
     CDS             786..1238
                     /locus_tag="PKW73_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73352.1"
                     /translation="MNDWKPILEGVSYFLEMLSLATGVPALLLCWLPAILGFKGYTPR
                     VILVSLLTIIYGIAVPGLTILVYQSLKNSNETFNFVCALLGYFAVFAIALTGPLCIAF
                     IPSFIARKHDLRIKKKVFLWNFLGLVVPYMWIGLIIWCGVVHREKDEA"
     gene            1380..2444
                     /locus_tag="PKW73_08445"
     CDS             1380..2444
                     /locus_tag="PKW73_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73353.1"
                     /translation="MKEDFESPSQQPAERVEAPTSAQTEAVGGLDGYNAARDAMKGAD
                     TATVPPLELYDSQAGSDSSGSKSAQKSQADGSSSGDNLGVGSGTESSQSGGDATGGKG
                     AESQAATGDGKSATDSAPSDQTPPSIDSSAGTQSDGKPSSDSQAAGNDGKSATDSAPV
                     DPNNKGIEASSDAENKGVGKEGNDTGKNGPDSDKSGKDEIKSQIDGSTADKFGGGENS
                     KDPSSSKSAEAMSKNPVNDLDDMTTTAPGGDPSMGDFSPPQGESNVNPGAGDGGTNKG
                     DSGSKPPVNENGKPNGDAKNPGAYESDKDPGDSKGDIKSQDGAPDKNGSQISPSQSLP
                     LGPSNTPASTGGSVSQRPQW"
     gene            complement(2466..>2855)
                     /locus_tag="PKW73_08450"
     CDS             complement(2466..>2855)
                     /locus_tag="PKW73_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73354.1"
                     /translation="YDEMSGVFSKEHGAIVAENLYIFGRSGKNHRLQKTLAHEAGHAL
                     DHMLRDASLTVEFIAAYENDRKLMSASQRQARQYFMQEGKRGPREAFADLVAALYGTE
                     DNFLDSKIAKVFPNCAKLIQSYLPAKD"
BASE COUNT          660 a          725 c          770 g          700 t
ORIGIN      
        1 ttctaggttt tctcactctg attcgccaga ctagcttcaa actaggtaga attatccttt
       61 gaaatgactg ccggggagta attatgagag tttgccacgc cgggtcttgc agtcttctgg
      121 caatttgtct tctgggcgcg gcaattactg cccaggctgc ccctctcgac aaaccgccgc
      181 cggtgccaaa acatggggct accggcagac cggtagagcc tgtagatagt gcaccaaagg
      241 caaaagacga gaataatccg gctttccata aagccctgct tgagattgcc gggcagtata
      301 ctggatatca attgtcggat gatctcctga gacgggcgcc tgaacgttgc gcattgccgg
      361 tgcaagagtt gagtcagagc gctgatggcg gtagccacgg caagaagttc tattatcttt
      421 atgtgaaaga ccttgctccc tatctcaagc atgataaggt caagcccggt caggctttgg
      481 taaaagagtc ctataaggct atccccacca agacggggcc ctcgcagaag gtagagtata
      541 aggtcggcga taagtatgga ctctttataa tgctcaagat gccgccgcag acagtcggca
      601 ctgatgaggg ttggatttat ggtaccgtta cgcctgatgg gaagaccgtg accggttcag
      661 ggcggatggc ctcatgcatg agctgtcatt tgtctgcacc ccatggaaga ctctttggtg
      721 tcaaggctgc tcggtagtgg ctgtagaagc ctggctcacg gacgctctca agaaaggagc
      781 aaatcttgaa tgattggaag ccgattttgg aaggcgtatc ctacttcctg gagatgctct
      841 ccctagccac cggcgtgcct gcgctcttgc tttgctggct gccggctatc cttggcttca
      901 agggctatac accgcgagtg attctcgtaa gtttgttgac catcatctat ggtattgctg
      961 ttccagggct gactattctc gtctatcagt ctttgaaaaa ttccaatgag actttcaact
     1021 tcgtttgtgc cttgctcggc tattttgccg ttttcgccat tgctctgacc ggcccgcttt
     1081 gtatcgcctt cattcccagt ttcatcgccc gaaagcacga cttacgcatc aagaagaagg
     1141 tgtttctgtg gaattttctg ggtctggttg tgccctacat gtggattggg ctgatcatct
     1201 ggtgtggcgt tgttcatagg gaaaaggacg aagcttaggc aagtgggact ttcacggtca
     1261 tttcacgtgg tgctgtgtaa cttgtaaggc atcaacctca cgcacagccc cttactagaa
     1321 tttttgcagt atcgcgtgaa atccaccgct ttaccccatt ttctttgaga gtatttacca
     1381 tgaaagaaga ctttgaatca ccttctcagc agccagccga gcgcgtcgag gctcctacct
     1441 ccgctcagac cgaagctgtt ggtggtcttg acggttataa tgccgcacgc gatgccatga
     1501 aaggcgctga taccgccaca gttcctcccc tggaattgta tgattctcag gccggcagtg
     1561 actcaagcgg cagtaagagt gctcagaaat ctcaggctga tggcagctct tctggtgata
     1621 atttaggtgt cggcagtggc actgagagtt cccaatcagg cggtgatgct acaggcggta
     1681 aaggtgcaga atctcaagct gccactggcg acgggaagag cgccactgat tccgctccga
     1741 gcgatcagac ccctccttcc atcgatagtt ccgctggtac tcaatccgat ggcaagcctt
     1801 cctctgactc ccaggcggcc ggtaacgacg gcaagagcgc tactgattcc gctcccgtcg
     1861 atcccaacaa caagggcata gaagcctctt ccgacgcgga aaacaaaggt gtcggcaagg
     1921 aaggtaatga caccggtaag aacggccctg acagtgataa gtctggaaag gacgaaatca
     1981 agtcacagat tgatggttct acagctgaca agttcggtgg tggcgaaaat tccaaagacc
     2041 ccagttccag caagtccgcc gaggcaatgt ctaagaatcc ggtcaatgac ctcgatgata
     2101 tgaccactac ggctcctggc ggtgatccta gcatgggcga tttttcaccc ccgcagggtg
     2161 aaagcaatgt aaatccggga gccggtgacg gcggcaccaa taagggtgat tccggcagca
     2221 agccgccggt taatgaaaat ggcaaaccca atggagatgc caaaaatcct ggagcctatg
     2281 aaagtgacaa agatcccgga gattccaaag gcgatatcaa atctcaagat ggtgccccgg
     2341 acaagaacgg cagtcaaatc tcgccctctc agagcctgcc cttaggacct tccaacaccc
     2401 ctgcgagtac aggcgggtca gttagccagc gtccgcagtg gtaacgagtc gagcggaggc
     2461 tcgtcctaat cctttgccgg caggtaggat tgtatgagtt tggcgcagtt tgggaagact
     2521 tttgctatct tcgagtctag gaaattgtct tcggtgccat agagggctgc aacaaggtcg
     2581 gcaaatgcct ctcttggtcc tcttttccct tcttgcatga agtattgccg agcttgcctt
     2641 tgcgaggcac tcataagctt gcgatcgttt tcgtaggcgg cgataaattc tacagttagc
     2701 gaggcatcgc gaagcatatg gtctagtgcg tgtcctgcct cgtgggcaag ggttttctgg
     2761 aggcggtggt tcttgccgga ccgaccgaag atgtagagat tttctgcaac tattgcgcca
     2821 tgctccttag aaaagacgcc gctcatctcg tcgta
//
LOCUS       DAOQNZ010000417         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19073_length_2847_cov_3.615688, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000417 DAOQNZ010000000
VERSION     DAOQNZ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19073_length_2847_cov_3.615688"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PKW73_08455"
     CDS             complement(<1..1126)
                     /locus_tag="PKW73_08455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA73355.1"
                     /translation="MKTCPECYSEYDDETKTCPVDSTPLKSVEMDPLVGTRLGDRYEI
                     VSVIGRGGMGVVYKARQEQMDKLMAIKMLHSHMVSDSEAVKRFYREAKTVSQVRHHHI
                     VTLYDFGMSSQGQPFLVMDFLEGMSLKDELKHHGPLSFERAERIFSQVVDALASAHAM
                     NVVHRDLKPENILLSTQNQNSDWVTLVDFGLSKLKEPKTQDAYTITKTGDVCGSPPYM
                     SPEQCLAASAVDPRSDIYSLAVVVYECLSGVLPYTAKSAIEMMDCHLYGSPIPFNQSS
                     QDMKVCSELTNFFVRSLQKEPEARQADIIEFGHELKEALSRDSIKLKSYKHRMEKASF
                     KDMESEAEALMSGTYPALGPSEGPRSVLASSRALELAQKTQ"
     gene            complement(1264..2436)
                     /gene="hemW"
                     /locus_tag="PKW73_08460"
     CDS             complement(1264..2436)
                     /gene="hemW"
                     /locus_tag="PKW73_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNA73356.1"
                     /translation="MIASIGKIPRSCYVHIPFCSHKCHFCDFAAFAGLEAREPEYFQV
                     LAREITTRLTDFQKENEAEIVLDTLFVGGGTPGLARIDELVFMKSTLEQFCTLSPDCE
                     TSLETTPHAITEAKALDWLQMGINRLSIGIESFQDSELKAMGRDHSRSQALAGIEAAV
                     RAGHQNISLDFMYGLPEQTLASFKDTLREAVRLAEYLPIQHISAYGLEIAVNAPLLLR
                     YPRDSKAYVDEDGFVAMFETLVETLEEAGFKQYEVSNFAREGFESRHNITYWKNEPYF
                     AFGVGAHRYVNGVRSANTKSFNVYMRDFDEGSTREAIDHRGEEMETLMLALRMRRGLD
                     LEDFQKRFQKDLLRLKGKEIAILKEEGLVQLSDTHLQITRKGLTVMNSIIGQLLTG"
     gene            2480..>2847
                     /locus_tag="PKW73_08465"
     CDS             2480..>2847
                     /locus_tag="PKW73_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73357.1"
                     /translation="MLRRISLPLNLLIALGLTFSFAQAQALTGTQLSLIKLMNTSPVT
                     VDLRSVGDVIIQLRRKVTDNPHDGNLRLRLGTYLYLAGDLQGAASEMKRAVAINPEDY
                     LAHIMLARVLDLSMDEASAEL"
BASE COUNT          598 a          735 c          780 g          734 t
ORIGIN      
        1 cctgggtttt ctgcgccaac tccagagccc gggatgaggc cagcacgctg cgcggtcctt
       61 cgctgggacc gagcgccgga taagtaccgg acatgagggc ttccgcttcg ctctccatgt
      121 ccttgaagga ggccttctcc atgcggtgtt tgtaactttt cagcttgata ctgtcccggc
      181 tgagggcctc tttcagttcg tgcccgaatt cgataatgtc tgcctgcctg gcttccggct
      241 ccttttgcag ggagcgtaca aagaaattgg ttagttcact acagaccttc atgtcctggg
      301 aagactggtt gaagggaatg gggctgccgt agaggtgaca gtccatcatt tcgatggcag
      361 acttggccgt atagggcagg acgccggaga ggcattcgta gaccaccacg gccagtgagt
      421 aaatgtcgga gcggggatcc acagccgaag cggccaggca ctgttccggg ctcatatagg
      481 gagggctgcc gcagacatcg ccggtttttg taatcgtgta agcgtcctga gtttttggtt
      541 ccttcagttt tgaaagtccg aagtccacca gggtgaccca gtcggaattt tgattttgag
      601 tggataagag tatgttctcc ggcttcaagt cccggtgcac tacattcatg gcatgggcgg
      661 aagcaagagc gtctaccacc tgcgagaaga ttctttccgc tctctcgaaa ctgaggggac
      721 catggtgctt gagttcatct ttgaggctca tgccctccag gaagtccatg accaggaagg
      781 gctgcccctg ggagctcata ccgaagtcgt agagggtgac gatatgatgg tggcgcacct
      841 gggatacggt ttttgcttcc cgataaaaac gcttcacggc ttccgaatcg gagaccatgt
      901 gcgagtgcag catcttgatg gccatgagct tgtccatctg ctcctggcgg gctttataaa
      961 cgacacccat gcctccccga ccgatgactg agacgatttc gtagcgatca ccgaggcggg
     1021 tgcccacgag cggatccatc tccaccgatt ttaagggggt ggaatcgacc ggacaggttt
     1081 ttgtctcgtc atcgtattct gagtaacact cagggcaggt cttcaatttg aagcgagctc
     1141 caactgagcc gattggtaaa attcagcttt gtttttagag ataaaggggg gagaggtcgg
     1201 ggagtctata tttatccata tatgggacta tctttctatc tatctattcc ccgttctttc
     1261 gttttatccc gtaagcaatt ggccgattat tgagttcatc acggtcagcc ccttgcgggt
     1321 aatttgtaag tgtgtgtccg ataattgcac caacccctct tccttgagaa tggctatttc
     1381 cttgcccttg agacggagca gatctttctg gaagcgcttt tgaaagtctt ccagatcgag
     1441 cccccggcgc atcctgagtg ccagcatcag tgtttccatc tcctcgcccc ggtggtcgat
     1501 tgcttccctt gtggagccct catcgaagtc tcgcatgtag acattaaacg actttgtatt
     1561 agcgctgcgc acgccgttca catagcggtg ggcgcccact ccgaaagcaa agtagggctc
     1621 gtttttccag taggtgatgt tatggcgact ttcgaagcct tctctggcaa agtttgagac
     1681 ttcgtattgt ttaaaaccgg cttcttccag ggtctccacc agggtttcga acatagccac
     1741 aaagccgtct tcatcgacat aggctttgct gtcgcgggga tagcgcagga gaaggggggc
     1801 gtttacggca atttccagtc cataggcgga aatgtgttga attggtaagt attcggccag
     1861 ccttactgct tccctcagcg tgtctttgaa ggaggccaga gtttgctcgg gcaaaccgta
     1921 catgaagtcc agggaaatat tctgatgccc ggcccttacg gcggcttcaa ttccggccag
     1981 ggcctgtgaa cggctgtgat cccggcccat ggccttaagc tcactgtctt gaaaggattc
     2041 gatgcctatg gagagccgat ttatgcccat ttgcagccag tcgagagcct tagcctcggt
     2101 gatggcgtgg ggggtcgtct ccagggaagt ttcgcaatca gggcttaagg tgcagaactg
     2161 ctccagggtg gacttcatga agacaagctc gtcgattctg gcaagacctg gggtgcctcc
     2221 gcccacaaag agagtatcga ggactatctc tgcctcgttc tccttctgga aatccgtcag
     2281 gcgggtggta atttcccggg ccagaacctg aaaatattcc ggttcccggg cctctagtcc
     2341 ggcaaaagcg gcaaaatcgc agaaatgaca cttatgtgag cagaacggaa tgtgcacata
     2401 acagctccgg ggtattttgc caatgcttgc tatcattgtt tccactctac ttcagtctcc
     2461 gctacaagct aatagttgca tgctccgtcg aatttcatta ccgctcaacc tgctaattgc
     2521 cctgggtctt accttcagtt tcgctcaggc ccaggctcta acgggtacgc agctgtcttt
     2581 aatcaagtta atgaatacca gcccggtgac tgtagatttg cgctctgtcg gcgatgtcat
     2641 cattcagctc cggcgcaagg tcaccgacaa tccccatgac ggaaatttaa gattgcgcct
     2701 tggtacttac ctctacctgg ctggggattt gcagggtgcg gccagtgaaa tgaaaagggc
     2761 ggtggccatc aatccggaag actatctggc tcacattatg ctggcccggg tgcttgatct
     2821 gtctatggac gaggcttccg ccgaact
//
LOCUS       DAOQNZ010000418         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19112_length_2844_cov_3.111510, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000418 DAOQNZ010000000
VERSION     DAOQNZ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19112_length_2844_cov_3.111510"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKW73_08470"
     CDS             <1..204
                     /locus_tag="PKW73_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73358.1"
                     /translation="PGITPGAPNPVPGIAPLAPNPVPGTPTNDGLSPNPVPEAASPSP
                     NPVPGITPGAPNPVPTQVPATNP"
     gene            complement(423..2180)
                     /locus_tag="PKW73_08475"
     CDS             complement(423..2180)
                     /locus_tag="PKW73_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNA73359.1"
                     /translation="MNRIKVGIVARGLIIVAIPLAFTCYFVATLLVLQNQAENAASKA
                     EHDREISTLISELLYDLYMGIGSVKLKADNFSTNNNRKVQILAAKEKVGKLLNLLQNE
                     DPSARNILLSVQADIDKGNQLISRAEKAFSSGNKVELLRLRLEAQALADRLISPELIA
                     LGERREAVSAGDPSIEQELREQMRTQLWASLIISIAATLTLSLITTRALTRRLADLTA
                     NSRRIASGEALVEAIPGSDEIAELDQVFHKMAGEIENLIQRERIILESAGDLILVTDN
                     KLNILKVSPSVENILAIKPNDLLSKSCLDLLAPTGNQVAREKLSKASAGEDTDNFEAR
                     LTKSTGEVIDFLVSLSRVPEEALLILIFHDVSRLKEVERFKQQVVSMVSHDLRSPLTA
                     VGHTLEMFESGMFGDLNQEGQALLRQAEHSTRKMSLLIVDLLDLDKIESGKLKLSIGS
                     VSCRTLMETAQTVSPEARIEIAECTGRVLCDLERTNQVFAGLIKFALKLCAAPASLLL
                     TSQVKSPQIEFSIAFNTSSLPEGQQASLFEKFVLPKEEHDTAGLALTISKALIELQGG
                     SIEYRNKLGFVFTLPEAKS"
     gene            2515..>2844
                     /locus_tag="PKW73_08480"
     CDS             2515..>2844
                     /locus_tag="PKW73_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73360.1"
                     /translation="MSHFENVELGKSAEPSMSDLASQALLADSMPPFKVAFKQEEKPI
                     VLATNDHRSGCSTCSTTDVPPVDSAPSSNTNENTNHIDFKPQMSQEQLQKQGQEQGQQ
                     QSAEAIAK"
BASE COUNT          659 a          712 c          695 g          778 t
ORIGIN      
        1 cctggcatta cgcctggggc gcctaatccg gtgcccggca ttgcgccttt ggcacctaat
       61 ccggtgcccg gcactcccac caatgatggg ctttcgccta atcctgtacc agaagctgcc
      121 tcaccctcac ctaatccggt gcccggcatt acgcctgggg cacctaatcc ggtgccgacg
      181 caagttcctg ctactaatcc ttgaacatga ttggcttgtc tgcttgccga tggtccatct
      241 atcttttgaa aaatccttca atgtaacaaa agtgattttg cgaaatatta catcagggtt
      301 tcgggacttt tcgtctttga aaggcttgct atgactgcgg tttagcggtc aagcagaatt
      361 aacttttccg gttacatgag tttagtgatg ccgccagcgg tatctgtcgc caggtctctt
      421 catcagcttt tagcttccgg cagcgtgaag acaaagccca atttatttct atattcgatg
      481 ctgccgccct gtaattcgat cagcgccttg ctgattgtca gtgccagtcc ggctgtgtca
      541 tgctcttctt ttggcaggac aaacttttcg aagagcgaag cttgctgtcc ctctggtagt
      601 gaggaagtgt tgaaggcaat tgaaaattca atttggggcg actttacctg actggtaaga
      661 agcaaactag ctggggcggc gcacaatttc agagcgaact ttattagacc ggcgaatacc
      721 tggttggtac gctcaagatc acatagtacc cggcctgtgc actcggcaat ttcaattctt
      781 gcttctgggc tcactgtttg cgctgtctcc ataagcgtcc gacaggagac agacccgatc
      841 gatagcttca gcttaccgct ttcgattttg tcgagatcga gtaaatcaac gattagcagc
      901 gacatctttc tggtggaatg ctcagcctgt ctgagaagtg cttgcccttc ctggttcaag
      961 tctccaaaca tgccggactc gaacatctca agggtatgac cgactgcggt caagggtgaa
     1021 cgtaagtcat ggctgaccat actcactact tgctgcttga agcgctcaac ttctttgaga
     1081 cgactgacat catggaagat caaaatcaaa agtgcttctt ccggtacacg gctcagggag
     1141 accagaaaat caataacttc tccggtggac tttgtcagcc gggcttcaaa gttatccgtg
     1201 tcctcgccgg ccgatgcctt gctaagtttc tcccgggcaa cttgattgcc tgttggcgcc
     1261 agcaggtcca gacaactttt agacagaagg tcgtttggct tgatagccag gatattttcc
     1321 actgatggac tgactttcag gatattcagc ttattgtcgg taacaaggat caagtctccg
     1381 gcgctttcaa gaataatacg ttctctttgt atgaggtttt ctatttcacc ggccatttta
     1441 tggaagacct gatcaagttc ggcaatttcg tcgctacctg gaatggcctc taccagagct
     1501 tcaccgctgg ctattcgcct gctgttggcc gtgaggtcgg ccagtcgcct ggtgagggct
     1561 cgggtggtaa tcaacgaaag cgtcagtgtg gccgcaatac taattatgag agatgcccag
     1621 agctgggttc gcatttgctc tctaagctct tgctcgatgg agggatcacc ggcggatacc
     1681 gcctctctgc gctcaccaag ggcaatcagt tctggtgaaa tgagccggtc tgccagtgct
     1741 tgcgcttcca gcctcagtcg cagcagttct actttgtttc ctgatgaaaa agccttttcg
     1801 gctcgactaa taagctggtt gcctttatca atgtctgctt gcactgaaag aaggatattt
     1861 ctcgccgacg gatcttcatt ctgtagaagg ttgagcagtt tccctacttt ttctttggcc
     1921 gccagtattt gaactttgcg attgttgttg gttgagaaat tgtctgcctt gagtttgacg
     1981 gagccgattc ccatatatag gtcgtaaagc agttcgctta tcagggttga aatttcccgg
     2041 tcatgctctg ccttcgaggc ggcattctcc gcctggtttt gcagcaccaa taaggtggct
     2101 acaaagtagc aggtaaaggc aagcggaatg gcaacgataa tgagacccct ggcgacaatt
     2161 ccaactttga tgcggttcaa ttgtttaagc tcacggcctg caggacctgt gcattttagc
     2221 actatcgccg ccgctctttc tttgaccact tttggtcacg catcagtcca taagagtctc
     2281 tcattagatc tatatccatt accagataga taagagatcc ttcccttgca gtgcttgcaa
     2341 acgcgcatgg gctgcgtaga tctacgaaga aaatctccac cggtggtggc aaattgccca
     2401 tttttagtca ttgtgatgaa ttactgttaa tccatcggaa agaccggtgt gtccggtgcg
     2461 aatcccgaga atcttgttcc ccaaataatt tatctagata aggagattgc agtcatgagc
     2521 cattttgaga acgttgagct tggcaaaagc gcagagccca gtatgagtga ccttgccagt
     2581 caggctttac tggctgacag catgcctccc tttaaggtcg ccttcaaaca agaagaaaag
     2641 ccgattgtac ttgctactaa tgatcaccgc tccggttgca gcacctgcag cacaaccgat
     2701 gttcctccgg tggacagtgc gccttcgtca aatacgaatg agaatacaaa tcatattgat
     2761 ttcaaaccgc agatgtcgca ggaacagttg cagaagcagg gtcaggaaca aggacagcag
     2821 cagagtgccg aggccattgc caaa
//
LOCUS       DAOQNZ010000419         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19124_length_2843_cov_3.617288, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000419 DAOQNZ010000000
VERSION     DAOQNZ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19124_length_2843_cov_3.617288"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..825
                     /locus_tag="PKW73_08485"
     CDS             34..825
                     /locus_tag="PKW73_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNA73361.1"
                     /translation="MTITYKNITTAREEAGQAFIGIATLNRPSVLNALNHELMTELVE
                     ALQEFDQDETVRAIVITGSDRAFAAGADIKEMCDETTVSMMLKDRLATWDKIKNIKKP
                     IIAAVSGFALGGGCELSMMCDIIIASETAQFGQPEINIGVIPGAGGTQRLTRVLGKYK
                     AMELTLTGRPFSAKEALELGLVNKVLPVELYLEEAKAMAREIAAKSPIAVRLAKEAVL
                     KSFDGTLDEGLTFERKNFYMLFSSEDQKEGMKAFLEKRKAVFTGR"
     gene            859..1656
                     /locus_tag="PKW73_08490"
     CDS             859..1656
                     /locus_tag="PKW73_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNA73362.1"
                     /translation="MSDEKILLTEKKNGVGIITLNRPDKLNAFNDELTFQLQDALKEM
                     EKDKEVRAIVITGAGRGFCSGQDLQSRSIAQEMGQRPSLGDSIKRRYNPIVIKLRRIE
                     KPIIAAVNGVAAGAGASVAFACDFRIVTEKTNFIQSFTKVGLIPDSGATFVLPRLIGL
                     TKAFELMLSADKLSAQEAQNLGVVNKIVAEEDLMKEAMALAEKLAMGPTKAFGLTKRA
                     VNKAIFDDLEELLEYEAGLQEVAGRSDDFQEGVKAFIEKRQPQYTGK"
     gene            complement(1686..2492)
                     /gene="dapB"
                     /locus_tag="PKW73_08495"
     CDS             complement(1686..2492)
                     /gene="dapB"
                     /locus_tag="PKW73_08495"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNA73363.1"
                     /translation="MTLKICLGGVSGWTGSAVAQAVLAASDMELVSCLSRKYAGQNLS
                     QLPGFAGAPSAVPVYDTVEAALACAPTVYIDYTSAEAVKGNVKAALNQGVHCVVGSSG
                     LSQEAYAELESLALAKGVGLIACGNFSITAALAKHFSLLAAKYLPHREIIDYSHAEKP
                     DAPSGTARELAEALAAVAENQMLFPVSATVGEPGARGAQIKGTPVHSLRLPGYVLAFE
                     SIFSMPEERLIIKHEAGKSAAPYVDGTMLAVRRVTSIKGLVRGLDTLLFS"
BASE COUNT          805 a          828 c          672 g          538 t
ORIGIN      
        1 cgagatgaaa tctaagctgg agaagagatg gtaatgacaa tcacctataa aaacatcacc
       61 acagccagag aagaagccgg acaggctttc atcggcatcg ccacactgaa ccgacccagt
      121 gtcctaaatg ctctcaatca cgaactaatg accgaactgg tggaagccct gcaagaattc
      181 gaccaggacg aaactgtgcg ggccatcgtc atcaccggca gtgaccgcgc ctttgccgcc
      241 ggtgccgaca tcaaagaaat gtgcgacgaa acaacagtgt ccatgatgct caaggaccga
      301 ctggccacct gggacaaaat caagaacatc aagaaaccaa taatcgctgc cgtaagcggc
      361 ttcgccttag gcggcggctg cgaactctcc atgatgtgcg acatcatcat cgcttccgaa
      421 accgcccagt tcggccaacc ggaaatcaat atcggcgtca tccccggagc cggcggcacc
      481 cagcgcctca cccgcgtact gggcaaatac aaagccatgg aattgaccct cacaggccgc
      541 cccttcagcg ccaaagaagc cctggaactt ggtctggtca acaaagtatt gcccgtggaa
      601 ctctacctgg aagaagccaa agccatggcc agagaaattg cggccaaatc tcccattgcc
      661 gtaagactgg ctaaagaagc cgtactaaaa tcctttgacg gcaccctcga tgaaggtctc
      721 acctttgagc gcaaaaactt ctatatgctc ttctcctccg aagatcaaaa agaaggcatg
      781 aaagccttcc tggaaaaacg caaagccgtc ttcaccggtc gctaacgctc aatttcactc
      841 acaaggaaaa gaaaaaaaat gtcggacgaa aagatccttc ttacagaaaa gaaaaacggc
      901 gtcggaatta tcactctcaa tcgccccgat aaactgaacg ccttcaatga cgagctgacc
      961 tttcaattgc aagacgctct caaagaaatg gaaaaagaca aggaagtaag agccatcgtc
     1021 attaccggcg ccggtcgcgg tttctgctca ggtcaggacc tgcaaagccg ttccatcgcc
     1081 caggaaatgg gacagcgccc ttctcttggg gacagcatca agcgccgcta caatcctatc
     1141 gtgatcaagc tgcgccgcat tgaaaaaccc atcatcgccg ccgtcaatgg tgtcgctgcc
     1201 ggtgccgggg cctccgtggc cttcgcctgc gatttccgca tcgtcactga aaaaaccaat
     1261 ttcattcagt ctttcacaaa agtcggtctc atccccgact ccggcgccac ctttgttctg
     1321 ccgcgcctga tcggtctgac caaagccttt gagctaatgt tgagcgcaga caaactctcc
     1381 gcccaggaag ctcagaacct tggcgtggtg aacaaaatcg tcgccgaaga agacctcatg
     1441 aaagaagcca tggctcttgc cgaaaaactg gccatgggtc cgaccaaggc cttcggtctc
     1501 accaaaagag ccgtcaacaa agccatcttc gacgacctgg aagaactctt agaatacgaa
     1561 gccggactgc aagaagttgc cggacgctcc gacgattttc aggaaggcgt caaagccttc
     1621 attgaaaagc gccagcccca gtacacaggc aagtaaccct gagggaagtt cccaggactt
     1681 agcgctcaag aaaacagcag ggtgtcgaga cctctcacaa gacccttgat agaggtgacg
     1741 cgcctgacag ccagcatagt gccgtccaca tagggggcgg cacttttgcc tgcttcatgc
     1801 ttaataatga gcctttcttc cggcatagaa aaaatggact caaaggccag cacataaccg
     1861 ggcaaccgca gcgaatgcac tggcgtaccc tttatctgcg cccctctagc cccaggctcc
     1921 cccactgtgg cagagacagg aaagagcatc tgattctcgg caactgccgc cagggcctca
     1981 gccagttccc gtgcggtgcc gctgggggca tcaggttttt cagcatggga atagtcaata
     2041 atctctctgt ggggcaaata cttggccgcc agaagggaaa agtgttttgc aagagccgcc
     2101 gtaatggaaa aattaccgca ggcaatcaga cccactccct tagccagagc caaactctca
     2161 agctcggcat aagcttcctg actcaagccg gaactgccca ccacgcagtg cacaccctgg
     2221 ttaagagccg ccttcacatt gcctttcacc gcctcggcac tggtgtagtc gatatacacc
     2281 gtcggtgcac aagcaagtgc agcttcgacc gtatcgtata ccggcaccgc ggacggtgcc
     2341 ccagcaaagc cgggcagttg gctcaaattt tgcccggcat acttacgcga gagacaagaa
     2401 accagctcca tatcggatgc agcaagaacc gcctgagcta cggctgaacc ggtccaacca
     2461 ctgacgccac caagacaaat tttcaaagtc acaattttct ccagaagtca caagccggga
     2521 aacgaatcaa tagaatgagc attcagcgta ggatgaggag ggaaacaacg gtcgtttaaa
     2581 ctaacagata cagggcaagc tgattgaaga agtctcccgg acaccgagct aatgcaaatc
     2641 agagcctcac cagactctca ttgccatcta taatacccga caacaatcca gtcagcccaa
     2701 aagatgggag ccacaagaac ctgatgcaat tcaagactta tattaccgct ctggcagtgt
     2761 tttgctcaac agccggcgca tcatatagcc aagaaataca gcgtgattgt cagtcttcgc
     2821 agaactctta tcgagtggtt tac
//
LOCUS       DAOQNZ010000420         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19187_length_2838_cov_3.688825, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000420 DAOQNZ010000000
VERSION     DAOQNZ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19187_length_2838_cov_3.688825"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PKW73_08500"
     CDS             complement(<1..1373)
                     /locus_tag="PKW73_08500"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HNA73364.1"
                     /translation="MKYCENILETIGSTPLVKLNKVTKGLKPTILAKVEVFNPGGSIK
                     DRPSLKMIEEAEKAGLLKPGGTIIEPTSGNTGTGLAQIAAVKGYKCILICPDKVAPEK
                     INLLKAYGAEVVMVPSTVSAGSPESYYSVANRLTMEIPGSFQPNQFANPNNPLAHYQS
                     TGPEIWEQTGGKITCLVAGAGTGGTISGTAKYLKEKNPNIKIVGADPEGSIYSGDTPG
                     SYKVEGIGEDFIPRNADLKIVDHFERISDKESFQMARRLAREEGLLVGGSCGTATCAA
                     LRVAETMTEEDVIVVILPDGGRGYLSKVYSDDWMRDNGFLPASGMSFYAKDLIAEKIK
                     DGRVPPMITVKPDDTVQHAIDLMEKYQIDQLPVITEAGQNVGHINDLVAMQIVFERKH
                     PEATHISSVMGRPYPQVDSDAEIDHVYRQFKLGTSMVILTKDGKAAGVLTKFDIVQHL
                     KAETGAET"
     gene            1429..>2838
                     /locus_tag="PKW73_08505"
     CDS             1429..>2838
                     /locus_tag="PKW73_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNA73365.1"
                     /translation="MTKSKYFFKPDDLSHFRFERDLGQPGAYPFTRGIYPEMYRTRVW
                     TMRQYAGFGDADATNRRFRYLLDKGQTGLSVAFDLPTQMGLDSDDPMALGEVGRTGVA
                     IDTLKDMERLFDQIDLGKVSTSMTINSTSAILLAMYRAVGIKQGVDPRLLSGTIQNDI
                     LKEYEARNTYIFPPRGSMRLITDIFEYCSKELPKWNTISISGYHIREAGATAVQELAF
                     TFANAIEYVRAAISRGLDVDDFAPQLSFFFVAQMNLFEEIAKFRAARRLWAKIMKEKF
                     NAKNDKSMTLRFHVQTAGSSLTLQQPDNNIVRTTIEALAAALGGCQSLHTNSKDEAIA
                     LPTEASALTALRTQQIIAFETGVGSVVDPCGGSYYIENLTSRIEAEVEEYLARIEEMG
                     GAIKAVETGFIQKEIQDSAYEDHQEVESKRKLVVGVNSFIDKEEEEIPVQRISPELES
                     KQVNYLREIRAGRDQEKVNA"
BASE COUNT          687 a          768 c          741 g          642 t
ORIGIN      
        1 gtttcggcac cggtctcggc tttcagatgc tgcacgatgt cgaacttagt gagaacaccg
       61 gcggctttgc cgtccttagt gagaatcacc atggaggtac cgagtttgaa ttgccgatag
      121 acatgatcga tttcagcatc agaatcgacc tgcggataag gtctacccat gaccgagctg
      181 atatgagtgg cttcgggatg cttgcgctcg aagacaatct gcatggcgac aagatcattg
      241 atatggccaa cgttctggcc ggcttcggta attacgggca gctgatcgat ttgatatttt
      301 tccatcaagt caatggcatg ctgcacggta tcgtcgggtt tgacggtaat catgggcggc
      361 acgcgaccat ctttgatttt ctcggcaatc aaatctttgg cgtagaagct catgccggaa
      421 gcaggtagga agccattgtc gcgcatccaa tcatcggaat agactttcga gagatagcct
      481 ctgccgccgt cgggcagaat cacaacgatc acatcttctt ccgtcatggt ctcggctacg
      541 cgcaaggctg cgcaggtagc agtaccgcag gaaccaccca ccaggagacc ttcttccctg
      601 gccaggcggc gcgccatttg gaacgactct ttgtcggaga tgcgctcgaa atgatccaca
      661 attttcaaat cggcatttct gggaatgaag tcttcgccga taccttcaac tttgtaggag
      721 cccggtgtgt cgccggagta aatcgaacct tccgggtcgg caccaacaat tttgatatta
      781 ggattctttt cttttagata cttggcagta ccggatatag tgccaccagt gccggcaccg
      841 gccaccagac aggtaatctt gccgccggtc tgttcccaga tttccggccc ggtcgactga
      901 tagtgcgcaa ggggattatt gggattggcg aactgattgg gctggaagga tccgggaatt
      961 tccatggtca ggcggttagc taccgagtag tagctctcgg gcgagccggc ggaaaccgtg
     1021 gaaggcacca ttacaacttc agcaccgtaa gccttaagca agtttatttt ttccggcgcg
     1081 accttgtcgg gacagatcaa aatgcactta taacccttga cggcggcaat ttgagccaga
     1141 ccggtaccgg tgttgccgga agtcggctcg ataattgtgc cgccgggctt gagcaggcct
     1201 gccttttcgg cttcttcaat cattttcagg gaaggtcggt ccttaatgga accacccgga
     1261 ttgaaaacct caaccttagc caggatggtg ggcttcagtc ctttagtgac cttgttaagc
     1321 tttacaagag gcgttgagcc gattgtttca aggatattct cgcagtactt cacgaggggc
     1381 ggtcctccct tacaatatat ggataaagta acggaatatt ataagctcgt gaccaagtca
     1441 aaatacttct tcaaacctga tgatctctct cactttcgct tcgaaagaga ccttgggcaa
     1501 ccaggtgcct accctttcac ccgcggcata tacccggaga tgtaccgtac acgggtgtgg
     1561 accatgcgtc agtacgccgg cttcggtgac gccgacgcca cgaaccgccg ctttagatat
     1621 ctcttagaca aggggcagac cggtctttcc gtcgccttcg acctgcccac ccagatgggt
     1681 ctcgattccg acgaccccat ggcccttggc gaggtcggac gcaccggcgt tgccatcgat
     1741 accctcaagg acatggaacg cctcttcgac cagatagacc tgggcaaagt ctcgacttcc
     1801 atgaccatca actccacaag cgccatcctg cttgccatgt accgggccgt gggtatcaaa
     1861 caaggtgttg atcccaggct gctttcaggc acaattcaaa acgacatatt aaaagagtac
     1921 gaagccagga acacctatat ttttccgccc cggggctcca tgcgcctcat taccgacatt
     1981 ttcgaatact gctcgaaaga gctgcccaaa tggaacacca tttccatctc cggctaccat
     2041 ataagggaag cgggcgccac ggccgtgcag gaactggcct ttaccttcgc caatgccatc
     2101 gaatacgtgc gggcagccat ctcccggggt cttgatgtag acgacttcgc cccccaactg
     2161 agtttctttt ttgtggctca gatgaatctc tttgaagaaa ttgccaagtt ccgtgcggcc
     2221 cgcaggctct gggccaaaat aatgaaagag aaattcaacg ccaagaacga caagtccatg
     2281 accctgcgct tccacgtgca gacggcaggc tcatctttaa ccctgcaaca gccggacaac
     2341 aacatcgttc gcaccactat tgaagccttg gcagcggcgc tgggcggttg tcagtccctg
     2401 cataccaatt ccaaggacga agccatcgcc ctgcccaccg aggcttcagc cctgacggcg
     2461 cttagaaccc agcaaataat tgccttcgag acgggagtcg gctcggtggt ggatccctgc
     2521 ggcggctcct actatataga aaacctcacc agccggattg aggcggaagt agaggagtac
     2581 ctggcccgca tcgaagagat gggcggcgct atcaaggctg tggaaacagg ctttatccag
     2641 aaagaaattc aagactccgc ctacgaagac catcaagaag tagaatccaa gcgaaaactt
     2701 gtggtgggtg taaattcctt catcgataag gaagaggaag aaattccggt gcagcgaata
     2761 tctcccgagc tggagagcaa acaggtaaac tacctgagag agataagagc cggtcgggac
     2821 caggaaaagg taaacgct
//
LOCUS       DAOQNZ010000421         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19213_length_2836_cov_3.734628, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000421 DAOQNZ010000000
VERSION     DAOQNZ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19213_length_2836_cov_3.734628"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PKW73_08510"
     CDS             <1..579
                     /locus_tag="PKW73_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73366.1"
                     /translation="GVLDARCKMIECADLNNAQQSIHSACRYIEGDGLKMALLRARLR
                     KVVKTRYESELNQLDALLTALKTQTSKQIGAQEITPDGEYRAQLDTILNRVRALARYR
                     STLLFVHRPELYTNSGVSALDLLERESGLALQERIYQLTKLVPPPEQHLLAYWSLVFE
                     LPALTNQGSGASRPDGEVSFKNGGPAQVAAVD"
     gene            complement(558..1268)
                     /locus_tag="PKW73_08515"
     CDS             complement(558..1268)
                     /locus_tag="PKW73_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA73367.1"
                     /translation="MPKILLVDSDSDLLLLASAHLFQEGYLVDTATNEPGAQDLLAYN
                     RYDVIILALDLPGTKGSEICFRLRLGRDTTPVIMLMEGEPVSHESGQQWGADDILHKP
                     FTSRELGRRIQRQLQKADKSARPAETLRCGNLELNCPNGILIKDGMEIHLTPIEFTLL
                     ELFMKHPLQYFSTDSLLTRVWSSQRHASDEAVRSSIKRLRRKIDNAEAEEAQLLIQSN
                     RQVGYRFRPPADQSTAAT"
     gene            1889..2449
                     /locus_tag="PKW73_08520"
     CDS             1889..2449
                     /locus_tag="PKW73_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73368.1"
                     /translation="MNAFEKTKQLLGSYVGEWSLFGVDGNGQPVLVHKWTDNLEVITV
                     EDLGDRVAVTAVDRLDFGNFQGMVTFREGYLKLPDGGCGQRFMEVGGQMIMQTEVSPG
                     VMVYPVSLADAELKSMGLDASKQQCLQHLGVRVVDGDTEFVTRLTFIVGGDREPQSES
                     GSASAPEKITVVPTLQGYHRRIKLSD"
     gene            complement(2494..>2836)
                     /locus_tag="PKW73_08525"
     CDS             complement(2494..>2836)
                     /locus_tag="PKW73_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73369.1"
                     /translation="KRNYLKLLDAGLKTPGNASSTGSQTDLPALLGKHPDHTIGALAS
                     TAAAVGCTALAVTAGAPEVIGLGILGAIGCGGAATANIGKIIEAEGNTRLEAAVNESL
                     RRISRPGAPSW"
BASE COUNT          640 a          668 c          799 g          729 t
ORIGIN      
        1 ggcgttctag acgctcgctg caaaatgatc gaatgcgcag acttgaataa tgcccaacaa
       61 agcatccatt ctgcttgtag gtatatagaa ggcgacggct tgaagatggc tttgctaaga
      121 gcaaggctga ggaaagttgt caaaacgcgc tacgagagtg aactcaatca acttgatgct
      181 ttgttgaccg ctctaaagac acaaacttca aaacaaatag gagctcaaga aattacgcct
      241 gatggtgagt atagagcgca actagataca attttgaacc gagttcgtgc acttgcccgt
      301 tatcgttcaa ccttgctctt cgtccacaga ccggaactgt atactaactc tggtgtcagt
      361 gcacttgatc ttctagaaag ggaaagcggt ttggcactgc aagagcgcat ctaccaactg
      421 actaaactag ttcctcctcc agaacagcat ttgctagcct actggtctct agtattcgag
      481 ttgccggctc taactaatca aggcagcggc gcgtccagac ctgatggaga ggtgtcgttt
      541 aaaaatggcg gccctgctca ggttgctgct gtcgactgat ccgctggtgg gcgaaagcga
      601 tagccgactt ggcgattact ttgaatcagc aactgggctt cctctgcctc ggcattgtct
      661 atttttcttc gcaagcgttt aatggaggat cgcaccgcct cgtcggaagc atggcgctgt
      721 gatgaccaga cgcgggtgag cagtgaatcg gtgctgaagt actgcagcgg gtgtttcatg
      781 aatagctcca gcaaggtaaa ttcaattggg gtgaggtgga tttccatgcc gtccttgatc
      841 agtatgccgt tcgggcagtt cagctcaagg ttgccgcaac gcagagtttc cgccgggcgt
      901 gccgatttgt ctgctttctg cagttgtcgc tggatccttc ttcccagttc tctgcttgta
      961 aaaggtttgt gcaaaatgtc gtcggcaccc cattgctgcc cgctttcatg gctgactggt
     1021 tcaccttcca taagcatgat caccggcgtg gtatcacgtc ccagtctgag gcgaaagcag
     1081 atctctgagc ctttggtacc aggcaagtcc agggccagaa tgatgacatc gtagcggtta
     1141 taggctagta gatcctgggc tcctggctcg tttgtggcgg tatctaccag atacccttcc
     1201 tggaagagat gagcggaggc aagcaatagt aggtccgaat cactgtctac cagtaagatt
     1261 ttgggcatga tcgggcttcc tagtgtaaga gtggttgtaa acacttcttt gactcccatc
     1321 gccttgatgt taaggcaggc gtgatgtgcg ggcttgagtt tgcacgactc agacctggtt
     1381 tgctggaggc ttgatcgtgc attgatgcga ggatagaaaa ttatcgtgac tttagggtgg
     1441 caggtagaaa ttggaaattc attttttgca gattgagctt tcccgactgc cctgcgtgac
     1501 attaatgtgg cgcccgcggt gccgaaatca gaggtgttgc gggaactcta aaaggcaggt
     1561 cagggagatt tgttcatccg gctggaggat tctcaaaaga atgctgtttc ttgcagtagc
     1621 agtgccgtaa tttaacaggt acctgctaat ttttcttcct cgccggaaag cctaacccca
     1681 tcttcagtcc aaacagataa tcgtttcttt gtcccatagg aagcagcacc ttgctgcttt
     1741 gggtttggcc agggcggagt gcgctggaag tttgagaggc aagtgcaaaa tgcagaacgc
     1801 ttgtgcccgg tagttgccga aggcccaagc gaaaccggct tccaatcggg agtcttcatc
     1861 aatttcaaaa atagaaatgg agggcaaaat gaatgccttt gaaaaaacaa aacaactcct
     1921 ggggagttat gtcggtgaat ggagtctatt cggtgtggac ggcaatggtc agcccgtcct
     1981 ggttcataag tggactgaca atttggaggt aataacggtt gaggatctcg gcgacagggt
     2041 ggcagtcacc gccgtggatc ggttggattt tgggaatttc cagggtatgg ttacctttcg
     2101 agagggctac ctgaaactgc ctgatggtgg ttgtggtcag cgctttatgg aagtaggcgg
     2161 ccagatgatc atgcagaccg aggtttcacc gggagtgatg gtttatccag tcagtttggc
     2221 tgatgccgaa ctaaagtcca tgggtcttga tgcttctaaa cagcagtgct tgcagcatct
     2281 gggtgtgagg gtagtggacg gagataccga gtttgttacc cgcctcacct ttattgtcgg
     2341 cggcgaccga gagccgcagt ctgagtcggg ctctgcctcc gcgcctgaaa aaattactgt
     2401 tgtaccaact ctgcagggtt atcatcgccg catcaaattg tcggattgaa cccggctcgg
     2461 gtaaacaatt atccttgctc ggcgtcggcc tttctaccaa ctgggggcgc cggggcggga
     2521 gattcggcga agagactcat tcacggccgc ttccagtctg gtattgccct cggcctctat
     2581 aattttgcct atattcgccg ttgccgcccc gccgcaacct atggctccga gaattcccag
     2641 accaattact tccggggcgc cggcagtaac agccagagct gtgcaaccta ctgctgcggc
     2701 cgtggaagcg agagcgccta tggtgtggtc gggatgcttg cccagaagcg cgggcagatc
     2761 ggtctgggag cctgtactgc tggcattgcc cggggttttt agaccggcat ccagaagttt
     2821 tagatagttt ctcttg
//
LOCUS       DAOQNZ010000422         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19240_length_2834_cov_2.763944, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000422 DAOQNZ010000000
VERSION     DAOQNZ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19240_length_2834_cov_2.763944"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKW73_08530"
     CDS             complement(<1..244)
                     /locus_tag="PKW73_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73370.1"
                     /translation="MTQNNSEEPGRDLIKVLLAEGVISQAQADLAARDAENMSMGLDD
                     VLLARRWVTEETLQRLAPWLFGGTGGSGVGSGAGAGG"
     gene            complement(267..614)
                     /locus_tag="PKW73_08535"
     CDS             complement(267..614)
                     /locus_tag="PKW73_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNA73371.1"
                     /translation="MDEKRSPGKRYGSKPFKKGPRRFGPSSGRVRQPVIQTTAPEDLA
                     EYILKVLAKDPDALQFEREQLNPGRSRINVTCDPLVTGRLIGKDGRTITALRSLIRAA
                     ASRYGKRVDIEVS"
     gene            complement(524..778)
                     /gene="rpsP"
                     /locus_tag="PKW73_08540"
     CDS             complement(524..778)
                     /gene="rpsP"
                     /locus_tag="PKW73_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNA73372.1"
                     /translation="MVKIRLKRVGAKKAPHYRIVAVDSRMRRDGMPIEELGYYNPRSK
                     ELNVNREAVEKWMKNGAQASDTVASLLKRDQGASAQVAGA"
     gene            complement(895..2445)
                     /gene="ffh"
                     /locus_tag="PKW73_08545"
     CDS             complement(895..2445)
                     /gene="ffh"
                     /locus_tag="PKW73_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNA73373.1"
                     /translation="MFDNLTDKLHGAFRSLTGNDKLTAENIEEAIREVRKSLLEADVS
                     LKVVKIFISNVRQKALGADVLTAVRPDQQFVKIVHDELVTILGGEHSPLNHKGNPGIV
                     MMVGLQGAGKTTACGKLANKLKAEGEKPLLVACDVQRPAAIHQLQTLGKQIDVPVFTI
                     EGNMDVQEIAEAAVKEAKEKGLSPVILDTAGRLQVDTPLMAELLIIDRFLKPQEKILV
                     VDSMTGQEAVNVAETFNTQLDLTGLLLTKVDGDARGGAALSVREAVGKPVKFISTGEK
                     LDNLETFYPDRMAGRILDMGDVVSLVERAQKTIDEKEATETIMEMFTKDLNFESFVKM
                     QGMMKGLGNMGDIMKMMGFGGMFGISTDQQKVIATEGEAMMNMYQIAINSMTKEERRN
                     PDLIDYKRRRRIAKGSGLKDNEIGKMLNDFQKMREVFRSFRQMMGGGGGFPGMPGMPG
                     MGGFPGMPGMGGFPGMGGPGMGGGGGRPGMPPMPPGFQGMGQRPAAKSGSGYSGGAFF
                     SKKKKKKK"
     gene            complement(2531..>2834)
                     /locus_tag="PKW73_08550"
     CDS             complement(2531..>2834)
                     /locus_tag="PKW73_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73374.1"
                     /translation="IEAQARQAVASLGYDPEYYVAVESTGFRPYDYYRPEEEHPQTNI
                     MVRTESGEIKELSQLSLAVEALVKGNYESYWLVYPQEAADKIKEIKEPALTHAKVF"
BASE COUNT          502 a          760 c          806 g          766 t
ORIGIN      
        1 ctccaccagc acccgccccc gagccaaccc cggaaccacc agtcccccca aaaagccagg
       61 gagccagccg ctgtaacgtc tcttccgtca cccagcgcct ggctagcaat acatcatcta
      121 gacccatgct catgttttca gcatctcgcg cggcgaggtc tgcttgagcc tgagaaatta
      181 caccttcagc caggagtacc tttatcaggt ctcttcctgg ctcttctgaa ttattttgag
      241 tcactatctg tctcccgtga aggtgttcag gagacttcga tatctactct tttaccgtag
      301 cggctggcgg ccgctctgat gagcgagcgc agcgccgtga tggtacgacc gtctttgccg
      361 atgaggcgtc cggtcaccag cgggtcgcaa gtgacgttga tacggctgcg acccgggttt
      421 agttgttcgc gttcaaattg tagggcgtca ggatctttgg ccagtacctt aagtatgtac
      481 tcggccagat cttccggcgc ggtcgtttgg atgaccggct gtcttacgcg cccgctactt
      541 gggccgaagc gccttggtcc ctttttaaaa ggcttgctac cgtatcgctt gcctgggctc
      601 cgtttttcat ccatttttca actgcttccc tgttgacgtt gagctctttg gaacgggggt
      661 tgtaataacc gagttcttcg atgggcatgc catcgcgtct catacgggag tctacggcga
      721 cgattctata atgcggggct tttttggcgc caacgcgctt aagcctgatt ttaaccactg
      781 actatctttc tccatttaat caaaaagact gcaaatagca attctaccag taggaagcgc
      841 caagccgtat gtccgaacag tatatgttgt taacctagcg caacttgccg acacctattt
      901 cttcttcttt ttcttcttgc tgaagaaggc gccgcctgag tagccgctgc cgcttttggc
      961 ggcggggcgc tggcccatgc cctggaagcc ggggggcatc ggaggcatgc cggggcgtcc
     1021 accgccgccg cccataccgg gaccacccat gccggggaag ccgcccatac cgggcatacc
     1081 ggggaagcca cccatgccgg gcataccggg catgcccggg aaaccaccgc cgccgcccat
     1141 catctggcgg aaggagcgga agacttcccg cattttttga aagtcattta acattttgcc
     1201 gatctcatta tctttgagac cgctgccttt ggcgatgcgg cggcgacgct tgtaatctat
     1261 aaggtcgggg ttgcggcgct cttccttggt catagagttg atggcaatct ggtacatgtt
     1321 catcatggct tcgccttcgg tggcgatgac cttttgctgg tcggtggaaa tgccgaacat
     1381 gccgccgaag cccatcattt tcatgatgtc gcccatgttg ccgaggcctt tcatcatgcc
     1441 ctgcattttc acaaagctct cgaagttgag gtctttggtg aacatctcca tgatggtttc
     1501 ggtggcttct ttttcgtcga tggtcttctg ggcgcgctca acgagggata ccacgtcgcc
     1561 catgtcaagg atgcggccgg ccatgcggtc ggggtagaag gtttccaggt tgtcgagttt
     1621 ttcgccggtg gagatgaact tgaccggttt gcctacggct tcgcgcactg agagggcggc
     1681 gccgcctctg gcgtcaccgt ctactttggt taagagtaag cccgtcaggt cgagctgggt
     1741 gttgaaggtt tcggcgacgt tgacggcttc ctgaccggtc atgctgtcta ccacgagaat
     1801 tttctcttgt ggtttgagga agcggtcgat gatcaaaagc tcggccatta ggggcgtgtc
     1861 tacttgcagg cggccggcgg tgtcgaggat gaccgggctc aggccctttt ctttcgcttc
     1921 tttgacggcg gcttcggcaa tttcttgcac gtccatgttg ccttcgatgg tgaagacagg
     1981 cacgtctatt tgtttgccca gggtttgcaa ctgatggatg gcggcggggc gctgcacgtc
     2041 gcaggcgact aagaggggct tctcgccctc ggctttcagt ttgttagcca gtttgccgca
     2101 ggccgtggtt ttaccggcgc cctgcaaacc gaccatcatg acgatgccgg ggttgccttt
     2161 gtggttgagg gggctgtgct cgccaccgag gatggtgacg agttcgtcgt ggacgatttt
     2221 gacgaactgc tggtcgggtc gcacggcggt gagaacgtcg gcgccaagtg ctttctgacg
     2281 cacgttggaa atgaagattt tgacgacctt caagctgacg tcggcttcca aaagagactt
     2341 tcttacttct ctgatggcct cttcgatgtt ctcggcggtg agcttatcgt taccggtgag
     2401 ggaacgaaag gcgccgtgca gcttgtctgt gagattatcg aacatttgga tgcttcctct
     2461 ttgcgcttct ctttagctta ttttgtcgca gtgtgtctca attttgtctg acgattttcc
     2521 gattttgaat ttagaatact ttggcgtggg tgagagccgg ctctttgatt tccttgattt
     2581 tgtcggctgc ttcttgcggg tagacaagcc agtatgattc gtagttgcct tttacaagcg
     2641 cttctacggc caggctcagt tgcgacaatt ctttgatttc accggattcg gtgcgcacca
     2701 tgatattggt ttgcggatgc tcttcttcgg ggcggtagta atcgtaaggg cggaagcccg
     2761 ttgattctac cgctacataa tattccgggt cgtagccgag agaggctaca gcctgccgcg
     2821 cctgggcttc gatg
//
LOCUS       DAOQNZ010000423         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19265_length_2832_cov_3.304285, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000423 DAOQNZ010000000
VERSION     DAOQNZ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19265_length_2832_cov_3.304285"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PKW73_08555"
     CDS             complement(<1..1190)
                     /locus_tag="PKW73_08555"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA73375.1"
                     /translation="MNTRRRKTILTLVSLMTLTVSLPSLLPEQAIAQSGKRIKSSSME
                     KIPCIRWVDDRVQPKAVLFCVHGLGLHKGVYEELGQTMAPQGIVTYAIDIRGFGSYVQ
                     KDPKAQVDFPGALDDIKRALTEIRRLHPSLPVIVLGESMGGAIAIHAAALNPTLIDGV
                     ISSVPAGDRFSNVDSGLKIGVHALFGGFDKPIDVGPMVIGRATKKEDLRKRWSSDPLA
                     RTKLSPNELIAFQRFMEANFDRAGQIKSCPVLFIQGCKDKLVRPAGTWKLFDSLATPN
                     KNLVLSTSAEHLIFEEGQFSKDDVGFITKWLDKSVINKGAAVADAPDSSEPEKVKQEP
                     EKVALAPAVKVKAEPGKVTPQPTITSDSPAISYWIELYRDGKVYRCNNKLAFKSGDAI
                     RFHV"
     gene            complement(1369..>2832)
                     /gene="dnaK"
                     /locus_tag="PKW73_08560"
     CDS             complement(1369..>2832)
                     /gene="dnaK"
                     /locus_tag="PKW73_08560"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNA73376.1"
                     /translation="TAAALAYGVEKKGKDETILVFDLGGGTFDVSILEVSDGLIEVKS
                     TNGDTHLGGDDFDHKLVQWIGEEFLKAESIDLRKDPQALQRLTEAAEKAKIELSSVTE
                     TNISLPFITANESGPKHLDMKLTRSRFNELTHDLVERCKHPIEQALLDAKMTYKEIDE
                     VVLVGGSTRIPAVKDLVKSLTGGKEPNQSVNPDEVVAVGAALQAGVLAGEVHDLVLLD
                     VTPLSLGIETMGGIFAKLVERNTTIPCHKTQMCSTAEDNQNAVDVYIYQGERSMARDN
                     KKLGNFRLDGIQPAPRGVPRIEVSFDIDANGIVQVSAKDLNTGKEQKVTITASTNLNK
                     EDIDRLVKEAQANAEEDQKVKAMADLRNEVDGLIYTVEKHIRENAEALTEDTKDKAHS
                     IIAKLKQNVGDNDEKEAREAMDQLRGHLVVMDQEANQYRSHKTDEKLKEEAEAKAKEE
                     AEKAEQTGAEGAGEAATDSESEHAEDSARDSEPAGAS"
BASE COUNT          516 a          716 c          820 g          780 t
ORIGIN      
        1 atgacgtgga aacggatggc atccccggac ttgaaggcaa gcttgttgtt gcaacggtaa
       61 actttgccgt cacggtagag ttctatccaa tatgaaatag ccggggagtc gcttgtgatt
      121 gtgggttgcg gcgtcacttt gcccggttca gctttcactt tgacggctgg tgccagggca
      181 accttttcag gctcctgctt cactttttca ggttcgctgc tatccggtgc gtcagctaca
      241 gccgcgcctt tgttgatgac tgatttgtct agccatttgg tgatgaagcc cacatcgtct
      301 ttactgaact gtccttcttc aaagataaga tgctcagctg aggtggatag caccagattt
      361 ttgttgggag tggccaggga atcaaagagt ttccaggtgc cggccggtcg caccagtttg
      421 tccttgcagc cctggatgaa gagcaccggg caggatttga tttgtccggc tcgatcgaag
      481 ttggcttcca tgaagcgctg gaaggcgatc agttcgttag ggctgagttt ggttctggcc
      541 agtggatcgc tggaccagcg tttgcgcaga tcttcttttt tggtggctct gcctatgacc
      601 atggggccaa catcgatagg cttgtcgaag cccccgaaga gagcatgcac gcctattttc
      661 agtccggagt cgacattact gaagcggtcg ccggccggca ctgaagagat aacgccgtct
      721 atgagtgtgg gattcaaggc ggcggcatga atggcgatgg caccacccat agactcgccc
      781 aacacaatca ccggcaggga cggatgcagt cggcgaattt ccgtgagggc ccgcttgatg
      841 tcgtcgaggg cgccggggaa atctacttgc gctttggggt ctttctggac atagctgccg
      901 aagccgcgga tatctatggc ataggtgact atgccttgag gagccatggt ctgaccgagc
      961 tcttcataga cgcctttgtg caaaccaaga ccgtgcacgc agaagagaac agctttaggc
     1021 tgtacccggt cgtccaccca gcggatgcag ggaatcttct ccatactgct ggatttgatg
     1081 cgtttgccgc tttgagcaat tgcttgttca ggcaagagcg aggggagaga aaccgtcaga
     1141 gtcattaaag agacaagtgt aaggatagtc ttacgtctgc gggtattcat gttttcctca
     1201 gtcatttgta cagggtacat tgcagctaat tttgtctgat ggcgccgagc taatagttcg
     1261 actttcttta ttggtccaag tcagacctac ggagaccaga ctgaaattag gcttgcctga
     1321 acaagcatta tattccgaat ccttattatt gcttggctgg tttattgctt agctcgctcc
     1381 ggccggctca gagtctctgg cggagtcttc cgcatgctca gattccgaat cggtggcggc
     1441 ttcgcctgcc ccttctgcac cggtctgctc agccttctcc gcttcttcct tggctttggc
     1501 ttctgcctct tccttcagtt tttcatcggt tttatggctt ctgtactgat tggcttcctg
     1561 gtccatgacg accaggtgtc cacgcagttg atccatggct tcgcgggcct ctttttcatc
     1621 gttgtcgcct acgttctgtt tgagtttggc gatgatgctg tgggctttgt ctttggtgtc
     1681 ttccgtcagg gcttcggcgt tctcgcggat gtgcttttcc acggtgtaga tgagcccgtc
     1741 cacttcgttg cgcaggtcgg ccatggcttt cactttctgg tcttcttcag cgttggcctg
     1801 ggcttccttc acgaggcggt cgatatcttc tttgttcaaa ttggtactgg cagtgatggt
     1861 tactttctgt tccttgccgg tattgaggtc tttggccgat acttgcacga tgccgttggc
     1921 gtcgatgtcg aaggaaactt cgatacgcgg tacgccgcgg ggagccggtt gaattccgtc
     1981 aagacggaag ttgcccagtt ttttgttgtc tctggccatg gagcgctcgc cctgatagat
     2041 gtagacgtct acagcattct ggttgtcttc ggcagtagag cacatctggg ttttgtggca
     2101 gggaatagtg gtattacgct cgaccaactt ggcgaagatg ccgcccatgg tttcgatacc
     2161 gagggagagc ggagtcacgt ccaggagcac caggtcgtgt acttctccgg ccaacacacc
     2221 ggcttgcagg gcagcgccaa cggcaactac ttcgtcgggg ttgacgctct ggttgggctc
     2281 cttgccgccg gtgaggctct taaccaggtc tttgacagcc ggaatacggg tggaaccgcc
     2341 caccagtact acttcgtcta tttctttgta ggtcattttg gcatcgagaa gggcttgctc
     2401 gatggggtgc ttgcaacgct cgaccaggtc atgggtcaat tcgttgaagc gggagcgggt
     2461 caacttcatg tccaggtgct tgggacctga ctcgttggct gtaatgaaag gcagggagat
     2521 attggtctcg gttaccgagg aaagttcgat tttggctttc tctgcggctt cggtcaaacg
     2581 ttgcagggcc tgcggatctt tgcgcaggtc gatactttca gccttgagga actcttcgcc
     2641 gatccattgc accagtttgt ggtcgaagtc gtcgccgccc aggtgggtgt cgccgttggt
     2701 ggacttgacc tcaatgagac cgtcggagac ttccaggatg gatacgtcga aagtgccgcc
     2761 gcccaggtcg aacaccagga tggtctcgtc cttgcctttc ttttccaccc cgtaagccag
     2821 agccgccgcg gt
//
LOCUS       DAOQNZ010000424         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19333_length_2826_cov_3.239986, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000424 DAOQNZ010000000
VERSION     DAOQNZ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19333_length_2826_cov_3.239986"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKW73_08565"
     CDS             <1..516
                     /locus_tag="PKW73_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA73377.1"
                     /translation="KPLIQWMHRKYRHKIPADKLIAISHHETVNFHGVEITAVPAHRP
                     LSRLGKTIMHLFARSSAPGKPVNGYYFEGVYHGGATKYSPTISHALKGKRIHTALLPI
                     GGKYATASPAEALKLAEEIRAERLVPMHWQPLKEQVFFRYQSSDLVKLAKTSGSVVDV
                     RALAIGEVLPG"
     gene            750..2204
                     /locus_tag="PKW73_08570"
     CDS             750..2204
                     /locus_tag="PKW73_08570"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA73378.1"
                     /translation="MPTLKQLQAMLSESLKAVGRTTQLPFGDPPEDYSLAVFKDRGKG
                     DLHWALYRGDGSTSMLVWDQVSMDPGYIHQLITAQFPGFDLKPVALRSQPTFQASSTR
                     ELQTAWDNYEQQSTSMRMRAKATLEGDLKNLQIPNVLQSIGMSKGTGRLEVESPSENA
                     VVYFVDGLPVHCELGNLDGGIAILELVGWEEGEFRFYPEAATEKRTIRKRLDTLLMEG
                     AALDDQYKAIRQKGIKENSFLIRVHDSLTESQFEKLLENGTGCDMNLQKQLYQQIDNK
                     SRLSDILRRFNLTKLDWVPIIFNFATCNMVNFVDRLADSAADDNAVSIDWSQSRAVDR
                     LLMRQDTGLYTYPTLLWMLEREFSRWESFSRPFSLILMEIAIRPTAVNQIIEPLPISG
                     IKEFAERVSRFKRKTDVLAHYETFGYALLLPETEGHLARNMAARLSELLESTPLSGGM
                     SGYPLMVSLGTACLPEDSLTVGSLLGRSRPKRID"
     gene            2640..>2826
                     /locus_tag="PKW73_08575"
     CDS             2640..>2826
                     /locus_tag="PKW73_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNA73379.1"
                     /translation="MGKSVGIDLGTTNSVVAVMEGGQPTVVSNAEGGRTTPSVVAFTK
                     SGERLVGQLARRQAVLNP"
BASE COUNT          665 a          691 c          778 g          692 t
ORIGIN      
        1 aagcccttaa ttcagtggat gcacagaaag tatcgtcaca agataccggc cgacaaatta
       61 attgccattt ctcaccatga aaccgtcaat ttccatggtg tagagattac cgccgtgccc
      121 gcccaccgcc ccttgagtcg tcttggcaag accattatgc acttgtttgc cagaagctcg
      181 gcgccgggca aaccggtgaa cggctattac ttcgaaggcg tctatcacgg cggcgcaacc
      241 aaatatagcc ctaccatctc ccatgccttg aagggcaagc gcattcacac ggctcttttg
      301 cccataggcg gcaaatatgc cacagcttcg ccggcggaag cccttaagct ggctgaggaa
      361 attcgggcgg aaaggcttgt gcccatgcac tggcagcctt tgaaagagca ggttttcttc
      421 cgttatcagt cttccgatct tgtcaagctg gctaagacct ccggttccgt cgtggatgtg
      481 cgggctctgg ccatagggga agtgttgccg ggttagggcg gcaaaatttc ctttggagag
      541 aatgatttaa attcgcctgc cgggcgggta gaagatgtaa ggacgggatt tgactctggt
      601 gaaaatgggg tcaaggccat ttcattgtgt ctattgcaac gccgccgttg gttatcggtt
      661 cgaaatacgc tctttaatac tgcctgatga tgcttggaaa tcgtagaaat gaagacagag
      721 ggttcaatcg gctgccgaag caggctggtt tgcctactct caagcaattg caggcaatgc
      781 tcagcgaatc tctgaaggcg gtggggcgta ccacccagct tccttttggg gatccacccg
      841 aagattattc cctggcggtt ttcaaagacc gtggtaaagg ggatctgcac tgggcccttt
      901 accggggtga tggaagcacc tcgatgttgg tctgggacca ggtaagcatg gaccctggtt
      961 acattcacca gttgatcacc gctcagtttc ccggctttga tttgaagccg gtggctctgc
     1021 gcagccagcc gacttttcag gcttccagca cgagagaact gcagacggcc tgggacaact
     1081 acgagcaaca gtcaacctca atgcgcatgc gggccaaggc caccctggaa ggtgatctca
     1141 agaacctgca aattcccaac gttttgcaat ctataggtat gagtaagggc accggtcgtc
     1201 tggaagtaga atcaccctct gagaacgccg tggtctattt tgtggacggt ttgccggtgc
     1261 attgcgagct tggcaacctg gacggcggca tagccattct cgagcttgtg ggctgggagg
     1321 aaggggagtt tcgcttttat cccgaggcgg ccactgagaa gcgcactatt cgcaagcggc
     1381 tggatacctt gctcatggaa ggggcggccc ttgacgatca atacaaggcg attcgacaga
     1441 aaggcatcaa ggaaaacagt ttcttgattc gcgtgcacga cagtttgact gaaagtcagt
     1501 ttgaaaaact gctggaaaac ggcacaggct gcgatatgaa tttgcaaaag caactctatc
     1561 agcagattga caacaaaagc aggctgtcag acattttgcg ccgcttcaat ttgaccaaac
     1621 ttgactgggt gccgattatc ttcaatttcg ccacttgcaa tatggtcaac ttcgttgacc
     1681 ggctggctga ttcggctgcc gatgacaacg ccgtcagtat cgactggagc cagagccgcg
     1741 ctgtagaccg tttgctgatg cggcaagaca caggcttgta tacctatccc accctgctct
     1801 ggatgctgga gagggaattc tcccgctggg agagtttcag tcgccccttc tccctgatcc
     1861 ttatggagat tgccattaga cccacggccg tcaaccaaat tatcgagcct ctgcccatct
     1921 ccggtatcaa agagtttgcc gagcgggtca gtcgcttcaa gcgcaagacc gacgtgctcg
     1981 cacattatga gaccttcggc tatgccctgc tcttgcccga gacggagggt catctggctc
     2041 gcaacatggc tgcccgtttg agtgaactgc tggagagcac gcccttgagc ggcggcatga
     2101 gcggttatcc cctcatggtt tccctgggaa ccgcctgtct gcctgaagat tccctcactg
     2161 tcggatcttt actggggcgt tccagaccaa agcgaataga ctgaacaaaa ctgggtatta
     2221 ggtaggtagt caataaagtt cttcgggaaa ggtgcttggg aaagttgctc gggggaggtg
     2281 cttggggagg ttgtagggaa gaaaaaattg aaattgaatt tggtttgggc ggaagcattt
     2341 ttactatttt tgcactttag aaccgttgtc gccgtcaagt ggtgaaataa tggtctctga
     2401 taaaattatg aatctttgta taagtttgga tgttcagccc gaccgggttg gcatcatgga
     2461 aaaagaccgg attgtggatg gttcaagcag gccaggggaa ctcaatcccc gaatttcaag
     2521 aagttgccaa tccaaatata tccaaatttt gtttttagct ggaacttatc ccaagtataa
     2581 ttcgtgggtg tctccagcct tagctttcaa agtaggtccg aagggagttt caaagtaata
     2641 tgggtaagtc agtaggcatt gatttgggaa ccaccaactc cgtcgttgcg gtgatggaag
     2701 gcggtcaacc gacagtggta tccaatgccg aaggtgggcg aaccactcct tctgtagtgg
     2761 catttaccaa atcgggcgag cgcctggttg gtcagttagc ccgtcgtcaa gccgttctca
     2821 atcctg
//
LOCUS       DAOQNZ010000425         2818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19458_length_2818_cov_3.144408, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000425 DAOQNZ010000000
VERSION     DAOQNZ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19458_length_2818_cov_3.144408"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1823
                     /locus_tag="PKW73_08580"
     CDS             333..1823
                     /locus_tag="PKW73_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNA73380.1"
                     /translation="MDFPLILDRESQEPLHRQLTAAMKEAITSGRLCAGQGLPSSREL
                     ASALGVSRDTVVRSYQDLTSQGYISLVPNCGTFVSVKPLTSDSKLKTSGLMEASEAEL
                     LSQATSAGLSDFARRLLSSSYAGATAADLPNLNYGAAPRDALPFKIWRQLLSQRSSIN
                     KSDSEFLDFDANEIFGYEPLREQVCRFLNRAKGLHCDPSQVVVFTDSQSGLDLIARVL
                     INPGDLVVLENPGYSGARELFQAYGAEISYVPVDENGLNTALLPVPAQPCKLIYVSPS
                     HQDPTGAVLTLSRRKELLAWAEKHCRFIVEDAFDSDYNYSANSLPALQGMTGSTPVLY
                     LYSFWKLLFPLVSTGCLVLPEPLVSAFGRAKRYVDRNFSLTEHQALSDFLAEGHLERH
                     IYKTRQLYRRRRQALIFALKQILGAAVRIPRESGGLHLSLHLSLPCSQEQVLDSARRV
                     GLMLVSASPYYAGGGPEEEYLVDFAGLPEEQSLAVVSAFAGNLELL"
     gene            complement(1908..2252)
                     /locus_tag="PKW73_08585"
     CDS             complement(1908..2252)
                     /locus_tag="PKW73_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73381.1"
                     /translation="MLIPSQIMVGLIAGGAQFGSHSGGSFEKQTSAQSQSESDNRQGS
                     NQADGAFRKQKRLQPIEKPHKPFTFGSRSIQTIGLRVSEFTGPKQVEEKKRVATHHET
                     SKPWQQATKFTT"
     gene            2251..>2818
                     /locus_tag="PKW73_08590"
     CDS             2251..>2818
                     /locus_tag="PKW73_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73382.1"
                     /translation="MTSAVSSATISTAEFVDAYPELFALYRQLVVSALSFQKAVHGNL
                     MQMIGARALRIEERFARKLKQAKSAYELSISELFRRYQDDERTIKGVLEQTLIDLAAE
                     RKLAQEPFVKEAEEAGVRLHERFGTDPIAKICYHELGLSKRAFDRERDGDSYREASIA
                     ITRHLSEQKLPSEKVYSDIGRLRREDGSR"
BASE COUNT          596 a          690 c          793 g          739 t
ORIGIN      
        1 acggtggtgg acagcgctcg gagaatcgcc gcgaacgcca caaggctcgc caggcggttc
       61 gctccggtca gtacgactga ggggagagcc gccatgacgg cagttcattc gctcagcgac
      121 cacttcagaa aagctctgga tccgcctgga ttcagggctt ttcttttttg gtttataata
      181 cgataagcaa tagtaggaat aatcggttga agagaaagct gatgctcgtg tgctgcagcc
      241 tgggctggat acacggatga tttagtcgct ttcaagcagg caagggtgtt gtgaattgtt
      301 atactgggcg ctcaccttat ctgcgtcgcc caatggattt tcccttaatt cttgaccgag
      361 aaagccagga gcctttgcac aggcagctca cagccgccat gaaagaggca atcaccagtg
      421 gtcgtctctg tgccgggcag gggcttccct cctctcgtga gcttgcttcc gccctgggtg
      481 tttcaagaga tacagtggtg cgcagttatc aggaccttac cagtcaaggt tacatctccc
      541 tcgtgcccaa ttgtggaact ttcgtaagcg tcaagcccct tacttcagac tcgaagctga
      601 agacctctgg gcttatggaa gctagtgaag cagagctttt aagccaggcg acgagcgccg
      661 gattgtcaga ttttgcaagg cgacttctga gttcctcgta tgccggtgcc acagctgccg
      721 atttgccgaa cttgaactac ggcgcggctc ctcgtgatgc gcttcctttc aaaatttggc
      781 ggcagttgct cagtcagcgc agttctatca acaagagcga ttcggagttt ttggactttg
      841 acgctaacga aatctttggc tatgagcctt tgcgagaaca ggtgtgccgt tttcttaatc
      901 gggccaaggg cttgcattgc gatcccagtc aagttgtagt gtttaccgat tctcaaagtg
      961 gattggatct gattgcaaga gtcttgatta atcctggaga tcttgtggtg cttgagaatc
     1021 cgggttattc aggtgctcgc gaattgtttc aggcctacgg cgcggagatt agctacgtgc
     1081 cggtagatga aaatggtttg aacaccgccc tattgcccgt tccggcacaa ccttgcaagt
     1141 tgatttatgt cagtccttcg catcaggatc cgaccggcgc tgtccttacc ctctcgcgca
     1201 gaaaagaact gctggcctgg gccgaaaaac attgccggtt catagtggaa gatgcatttg
     1261 acagtgatta caactatagc gccaattcac ttccggcctt gcagggaatg accggctcca
     1321 ctccggtttt gtatttgtac agcttctgga agcttctctt tcctctggtc agtaccggct
     1381 gtctggtgct gcctgagccg ctggtgtcgg catttggacg agccaagcgc tatgttgata
     1441 ggaatttttc cctgactgag caccaggctc tttcggactt tctggccgag gggcatctgg
     1501 agaggcatat ttataaaacc aggcagcttt acaggcgccg caggcaggcg ctcattttcg
     1561 cattgaagca aattcttggt gccgccgtca ggatccctcg ggaaagcggc ggtcttcatc
     1621 tgagtctgca tctatccctt ccttgttcgc aggagcaggt gctcgattct gctcggaggg
     1681 tgggcttaat gctggtgtct gcctctccat actatgccgg agggggccct gaggaagagt
     1741 atttagttga ttttgccggg ctgccggaag agcaatccct cgcggttgta tctgcctttg
     1801 ccggaaattt ggagctcctg tgatgattca gagatgtggg gggcttgccg tccggtgact
     1861 gtgaaaatta accgtccggt atcaagtttg cgaagtgcat ttttggctta cgtggtgaat
     1921 ttggttgctt gttgccatgg ttttgaagtt tcatggtggg tagcgacccg ttttttctct
     1981 tccacctgct tgggacctgt aaattctgaa acccgcaagc cgatagtttg aattgagcgt
     2041 gagccgaaag tgaacggctt atgtggcttt tcgattggct gcaggcgttt ttgtttgcgg
     2101 aatgcaccgt cagcttggtt gcttccctgc cggttatcgg attcagattg ggactgcgca
     2161 ctagtctgct tttcaaaaga accgccggaa tggctgccga attgggctcc gccggcaatt
     2221 aaaccaacca ttatttgtga aggaattaac atgacttcag cagtatcttc cgcaactatc
     2281 agcacggccg aattcgtaga tgcttacccg gagctatttg ctctctaccg gcagcttgtt
     2341 gtctccgcgc tttcgtttca aaaagctgtg cacgggaatc taatgcaaat gattggtgct
     2401 cgtgctctga gaatcgagga gcgcttcgcc cggaagctca agcaggctaa aagcgcctac
     2461 gagctttcaa taagtgaact tttcaggcgc taccaggatg acgaacggac catcaaaggg
     2521 gttttggagc aaaccctgat tgatctggcc gctgagagga aacttgccca ggagccgttc
     2581 gtaaaggagg ccgaggaggc cggcgtgcgc ttgcatgagc ggtttggcac cgatcccata
     2641 gcgaagatct gctatcacga gcttggtctt tcgaagagag catttgaccg ggagcgggat
     2701 ggagattcat acagggaggc tagcatagcc ataaccaggc accttagtga gcagaagctg
     2761 ccttcagaga aggtctattc cgacatcggt cgcctcaggc gcgaggatgg cagccgcc
//
LOCUS       DAOQNZ010000426         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19538_length_2812_cov_3.815742, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000426 DAOQNZ010000000
VERSION     DAOQNZ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19538_length_2812_cov_3.815742"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKW73_08595"
     CDS             complement(<1..222)
                     /locus_tag="PKW73_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73383.1"
                     /translation="MEFSQLNFLDQKILVPAGCLVLFLLIFFPRLKNMMEITVLKELT
                     AMVGPPTDVNPREVYSKQNYLEAAKSVQRA"
     gene            complement(388..2109)
                     /locus_tag="PKW73_08600"
     CDS             complement(388..2109)
                     /locus_tag="PKW73_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73384.1"
                     /translation="MDNTVTKPESDRSEGQLLKHRRLEPAETVLGELTSAPMFQEGLR
                     HLRNLDAAEKSRPLPEDLGAAVELRKGDGAVLTLQEGLVCGIKNGSNCLSIDYEKQGG
                     KVVFDENGLAKVAKVRALVGGNECEIDAALLRSEGQLSALTVVQKGDGLGSVVLKTGD
                     GKTSTLRSDFCRVEHEQVALADGRSVERVTALHRPNGSQVIYNYDQRQPLKLQQVVER
                     YRTSLGKVVTQYTDRVGESEKFVTHTQVEGEGKDGGQNLKPTWRTDLQMTQDGDLAYK
                     ELNEKFFMGPRNEGDGAARDLQAAREEFLRVASANKVFQGNENTILGWMNGFERRVDG
                     FSRRGWMAPSDLDLADSYMNLSRIFTDSVSGRASVVGAQERRLLVESALKELADPKRY
                     INQGSIGTCSLNSVESNLAGRRPQELTRWLREASTKGYVTSRGVNTRGERVKVQLSEA
                     QLNYEPSWNRSYSNQIFQFASIAALGYRSRGYDYGGTTNDQLNYVSRLACGQNMRILD
                     NWMGRGARKADIVDALKNRGSVAYIVPGHCMAIDDYDPKTDRFYVNNWWGGSRDGWYS
                     ARGLGLR"
     gene            complement(2121..>2812)
                     /locus_tag="PKW73_08605"
     CDS             complement(2121..>2812)
                     /locus_tag="PKW73_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73385.1"
                     /translation="CDRGGGGGGGGGGGELPETDNTDTNDKSINDRRTDVTNNGRFSE
                     LAGKLTDMNPVKYSNWMADVMPDGFMEEMGPPPWDPKKLAAYMAKHAKEIQEKMGKRA
                     SALDKQGDKEGSKAINDFAAGFEKTANNPAALEAFAGTFANFAERAESGKANPADVHA
                     MFDNNSNLEGAIRRSQMLESAKKYDTSAGGDADLSKLSPDNAASLIADLQRAPVPKVK
                     PLEGFSILPQK"
BASE COUNT          604 a          845 c          714 g          649 t
ORIGIN      
        1 agcacgctgc accgacttgg ctgcttccag ataattctgc ttggaataaa cttccctggg
       61 attaacgtcg gtgggcggac cgaccatggc cgtcagttcc ttcaggacag tgatctccat
      121 catgttcttg agacgcggaa agaagatcaa aaggaaaagc accaggcagc cggccggtac
      181 aagtattttc tgatctaaaa aatttagctg ggaaaattcc atagtcgcct ttaagccaca
      241 gttcaaagtc gtcagtcagg gggtagcact gcactcagat tccacatacg ttattcccgg
      301 cccggcaata acttaaaaac cttcctgaat tcaagcttcg agcgcctgat aaatcttggc
      361 ggccaggtct gagcccagac cattaaatca gcgcaaacct aatccccgag ctgaatacca
      421 gccatcccgc gagccgcccc accagttatt gacatagaag cggtcggttt tggggtcgta
      481 atcatcgata gccatgcaat gaccgggcac aatataggca acgctgcctc gattcttgag
      541 ggcatccacg atgtccgcct tcctggcgcc gcgccccatc caattatcga gaatgcgcat
      601 attctgaccg caggccagac ggctcacata gttgagttga tcattggtgg tgccgccata
      661 atcatagcca cgggagcggt aaccgagggc ggcgatggaa gcaaactgga agatctgatt
      721 ggaataagaa cggttccaac tgggttcgta attgagttga gcctcgctca gttgaacctt
      781 gacccgttca ccccgggtat tgacgcccct ggatgtgaca tagcctttgg tgctggcttc
      841 tctcaaccag cgggtcaact cctgggggcg gcgaccggcc aggttggatt ccaccgagtt
      901 gagggagcag gtgccgatac taccctggtt gatgtaacgc ttgggatcgg caagctcttt
      961 caaggcactc tccaccagca agcgccgctc ctgggcgccc actacactgg cccgaccgct
     1021 caccgaatcg gtaaaaatac gactcaaatt catgtacgaa tcagccaggt caaggtccga
     1081 aggagccatc cagccccgcc gactgaaacc atccacgcgc cgctcgaaac cattcatcca
     1141 gcccagaata gtgttttcat tcccttgaaa aaccttgttg gcactggcta cccgcagaaa
     1201 ctcctcccgg gccgcctgca gatctcgggc ggcgccgtca ccctcgttgc gcggtcccat
     1261 gaaaaacttc tcgttcagct ctttgtaggc caaatcaccg tcttgagtca tctgcaagtc
     1321 agtgcgccag gtcggtttta gattttgccc gccatccttg ccctctccct ccacctgggt
     1381 atgggtgaca aatttctcgc tctcgccaac gcgatcggta tattgcgtta ccactttacc
     1441 cagagaggta cggtagcgct ccaccacttg ctggagcttg agcggctgcc tctggtcata
     1501 gttgtagata acctgggatc cgttggggcg atgaagcgcg gtgacacgct ccacgctgcg
     1561 accatcggcc agagccacct gttcgtgctc gacacggcag aaatcactgc gcaaagtaga
     1621 ggttttaccg tcgccggttt taagaacaac agaaccgaga ccgtcgcctt tctgcaccac
     1681 ggtcaacgcg gaaagctgcc cctccgaacg aagcagagca gcatcaatct cgcattcatt
     1741 gccgcctacc agagctcgca ctttcgctac tttagccaga ccattttcat cgaagacgac
     1801 cttaccgcct tgcttctcgt aatcgataga gaggcagttg ctaccgtttt tgataccaca
     1861 gaccagaccc tcctgcaaag taagaactgc accatcaccc ttgcgcagtt ccaccgccgc
     1921 ccctagatcc tccggcaggg ggcggctctt ctcagcagca tccagatttc tcaaatgcct
     1981 cagaccctcc tgaaacatcg gcgcagaggt aagctcgcca agcacggttt cagcaggctc
     2041 cagtcgcctg tgcttgagga gctgcccctc gcttctatca gattcaggtt tggtcaccgt
     2101 gttatccata agtcactcaa ctacttctgc ggcaagatgg aaaaaccttc caggggcttc
     2161 actttcggaa ccggcgcccg ttgcaggtcg gctatcagac tggcggcatt atcgggcgaa
     2221 agtttggaga gatcggcgtc gccaccggca ctggtatcat atttcttggc gctctccagc
     2281 atctgagagc gccttatggc gccttccagg ttactgttgt tgtcgaacat ggcatgtacg
     2341 tcggcgggat tagctttgcc gctctcggca cgctcggcaa aattggcaaa agtgccggca
     2401 aaagcttcca gggcggcagg attgttagcc gtcttctcga agccggcggc aaaatcgttt
     2461 atggctttag agccttcctt gtctccctgc ttgtccaggg ccgaggctct cttgcccatt
     2521 ttctcctgga tttccttggc atgcttggcc atgtaagcgg ccaacttctt ggggtcccag
     2581 ggaggcggtc ccatttcttc catgaagccg tcaggcatca catcggccat ccagttactg
     2641 tacttgaccg gattcatatc cgtcagtttg ccggccagtt cgctgaagcg gccattgtta
     2701 gtgacgtccg tgcggcgatc gttgatgctc ttgtcgttag tgtcggtatt atcggtttcc
     2761 ggcagttcac cgccgccgcc accgccgccg cctccaccgc ctctgtcgca gc
//
LOCUS       DAOQNZ010000427         2810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19572_length_2810_cov_3.242831, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000427 DAOQNZ010000000
VERSION     DAOQNZ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19572_length_2810_cov_3.242831"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="ispG"
                     /locus_tag="PKW73_08610"
                     /gene_synonym="gcpE"
     CDS             complement(<1..1000)
                     /gene="ispG"
                     /locus_tag="PKW73_08610"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNA73386.1"
                     /translation="MSKAAIVRRKTRQVQVGDKYIGSDHAVLVQSMTNTPTEDPAATA
                     EQIIDLYNAGSEIVRITVNTREAAKAVPEIVKLLEKQGVSVPLVGDFHYNGHLLLSDF
                     PDCARLLAKYRINPGNVGRGEKHDDNFKQMIDAAIKWNKPVRIGVNWGSLDQDLLASM
                     MDENAASKEPLDGHAVLIEAIVESALSSARLANSYGLSSDKIILSAKVSEVPDLITVY
                     RKLAKKCDLALHLGLTEAGMGMKGTVSSAAALSILLFEGIGDTIRVSLTPQPGAPRIE
                     EVQVCQQILQSLDIRSFKPQVTACPGCGRTTSTVFQELARDIEAHLTQKRAHWQKSY"
     gene            complement(1183..1716)
                     /locus_tag="PKW73_08615"
     CDS             complement(1183..1716)
                     /locus_tag="PKW73_08615"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNA73387.1"
                     /translation="MLHHLNYKLPVFKLMMVKEEKKQKNPLPSIRSALDAAKLLEPLC
                     FAPEEHFVALHLNSKFEVIGLHEVSHGTLSASLVHPREVFKAALVANSYAILVCHNHP
                     SGAKLSASREDLTTTRTLIDAGKLLGVSVLDHLIVGPQQQSDIVRKKKRESRILPLSI
                     REEHPELWQSDVDCLSA"
     gene            complement(1827..>2810)
                     /locus_tag="PKW73_08620"
     CDS             complement(1827..>2810)
                     /locus_tag="PKW73_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNA73388.1"
                     /translation="QFLSVDLLSQLPFTIVWTAHSMFRAVGNSRVPMLNWLAMAVVIV
                     SLDLFLCLKLHMGIAGIGWAWLGGGMVGTILNLVLLTQSDLKRSLDLAPTLGAIFGGK
                     KEELKRTLDWMKRILNVGIPTCIQDLAWVMGNFALFSIFALTQNPTDSEAAWTIGFRL
                     EEMICTLPLHAIGCSVGTIIGQNLGAGKPERAERAGWQATQLSLLLLVPITIVMYINA
                     EPIARLMSQDREVVMLTADYLRIVGLCQPLVACWIVLFGAMTGAGYTKWPMWIGILCL
                     TVIRLPLSYLLVHNYNMGTNGIWMGISASAAVIGMLAILRFRSGVWKTQKV"
BASE COUNT          611 a          688 c          800 g          711 t
ORIGIN      
        1 ggtaggactt ttgccagtgg gcgcgctttt gcgtaaggtg cgcttcgatg tctctggcta
       61 gttcttgaaa gacggtgctg gtggtgcgtc cacagccagg gcaggctgtc acctggggtt
      121 tgaaggaacg gatatcgagg gattggagaa tttgctggca gacctgtact tcctcgatgc
      181 gaggggcacc gggttgagga gtaagggaga cgcgaatggt atcgccgatg ccttcaaaga
      241 gcagaatgga aagggcggcc gccgatgaaa cagtgccctt catacccata ccggcttccg
      301 tcagacccag gtgcagtgcc agatcgcatt tcttggccaa cttgcggtag accgtgatca
      361 ggtcaggcac ctctgaaacc ttggcgctca ggataatctt gtcgctgctc agtccgtaag
      421 agtttgccag tctggctgaa gaaagcgcac tctctactat ggcttcaatc aagacggcgt
      481 gtccgtccag gggctcttta ctggcggcgt tctcatccat catggaagcc agtagatctt
      541 gatcgagtga gccccagttg actcctattc gtaccggttt gttccatttg atggcggcat
      601 cgatcatttg tttgaaattg tcgtcgtgct tctcgcctct gccgacattg cccggattga
      661 tgcgatattt tgccagtagt ctggcgcagt cgggaaaatc ggagagaagc aaatgaccgt
      721 tgtagtgaaa gtcaccgacc aggggcacgc tcactccctg tttttcaagc agtttgacga
      781 tttccggtac ggctttagcc gcttctctgg tgttgacggt gatgcggaca atttctgaac
      841 cggcattgta gagatcgatt atctgctcgg ccgtagccgc cggatcttcg gtgggcgtgt
      901 tggtcatgga ctggacaagc accgcatggt cggagccgat gtatttgtcg cccacctgga
      961 cctgtctggt tttgcgtctg acaattgccg cttttgacat gagcctctgt attgccttat
     1021 ctgtgtgcag attcttagat gattttaccg ggctgaaggt gcccgccatc tgaatatttc
     1081 acaagaaaat aacaggaaag tcgcctgagc agcttctgtg ccttaagttc gacttatctt
     1141 ggactgcttt tccctggcta gaagccggaa atttggcggc tttcatgcac tgaggcagtc
     1201 gacatcgctt tgccacagtt ccggatgttc ttctctgatg ctcaggggca ggatgcggct
     1261 ttctcgtttt ttctttctta ctatgtcgct ttgttgctgg ggaccgacaa tcaggtgatc
     1321 tagaaccgat acgccgagaa gctttccggc atcgatgagg gtgcgggttg tggtcagatc
     1381 ttccctggag gcgcttaatt tggcgccgga gggatgattg tggcagacta aaatggcgta
     1441 gctgttggca accagggcgg ccttgaaaac ctctcgggga tgcaccagcg aggctgataa
     1501 ggtgccgtgg gagacctcgt gcagaccaat tacttcgaac ttgctgttca gatgcagggc
     1561 cacgaaatgt tcttccgggg caaagcagag aggctcaagc agtttggcgg cgtctaatgc
     1621 cgagcgtatt gaaggaagcg gatttttttg tttcttctct tctttgacca tcattagttt
     1681 gaagaccggt aatttgtaat tgaggtggtg aagcatcttc cctccttgag ttcaggcgac
     1741 agtggatgtt aaataagacg gtgcggccat gaaaaaagtt catgaccaca ccgtcttttt
     1801 atcttcctta gctatggcaa aagtctctag actttctgcg ttttccacac tcccgaacgg
     1861 aatctcagaa tggccagcat gccgattacg gctgcggaag cggagattcc catccatatt
     1921 ccgtttgtgc ccatattata gttgtgcaca agcaggtagg agagcggtag tctgatcacg
     1981 gtcaggcaga gtatgccaat ccacatcggc cactttgtat aaccggcacc ggtcatagct
     2041 ccgaagagta caatccagca ggctaccagc ggttggcaca aaccgactat gcgcagataa
     2101 tcggctgtaa gcattactac ttccctgtct tgtgacatca agcgagcgat tggctcggca
     2161 ttgatgtaca tgacgatggt gattggcacc aggagaagta ggctcagttg tgtggcctgc
     2221 cagcctgctc tttctgcacg ctccggtttg cctgctccca ggttctgtcc aatgatggtt
     2281 cccactgaac agccgatggc atgcaagggc aaagtacaga tcatctcttc caatctgaaa
     2341 cctatggtcc aggctgcctc ggaatcggtg ggattctgag tgagggcaaa gatggagaaa
     2401 agggcaaagt tgcccatgac ccaggccaga tcctgaatgc aggttggtat accgacattc
     2461 aagatgcgct tcatccaatc tagagtgcgc ttgagctctt cttttttgcc cccgaagatg
     2521 gcacctaagg tgggcgccag atccagcgag cgcttcaggt ctgattgagt gagcaggacc
     2581 aggttgagga tagtgccgac catgccgccg cccaaccagg cccaaccgat accggctatg
     2641 cccatatgga gcttgaggca gaggaagagg tcgagtgaaa ctatgaccac tgccatggcc
     2701 agccagttga gcatgggcac tcggctattg cctacggccc ggaacatgga gtgggccgtc
     2761 cagactatgg tgaagggcag ttgcgatagc aggtctaccg agagaaactg
//
LOCUS       DAOQNZ010000428         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19598_length_2808_cov_4.054123, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000428 DAOQNZ010000000
VERSION     DAOQNZ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19598_length_2808_cov_4.054123"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKW73_08625"
     CDS             complement(<1..488)
                     /locus_tag="PKW73_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/calcium exchanger protein"
                     /protein_id="HNA73389.1"
                     /translation="MEQNFVFAEFALCAAVILVTGSKLSKHADVIADATGLGKSWVGL
                     VMLATVTSLPELLTGISAVTLNDLPNLAVSGTLGSCMFNMLVIASLDLFSKHRPVSNM
                     VQQGHQLSAGFGIVLLALAAIDILFGKYLPSIKQLNSIDPLSILFIIVYLVAMRLIYS
                     YE"
     gene            complement(489..2390)
                     /locus_tag="PKW73_08630"
     CDS             complement(489..2390)
                     /locus_tag="PKW73_08630"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA73390.1"
                     /translation="MPESCLRQNGPCKGKDHKTRVFLSLSLAVAGVFTSVALFPGQAL
                     AETHGSPKVEKPSTTANHLATGLNDYEQGNFGAALHAFKAAALDKSLSPLDRIKAQVA
                     AADSAYQCGEYLTALNMHKRNLTVLKAVSQAEPMSTSRKGKTGSAKEILLPDQDREQL
                     LAETYCDIGEALYELDKHDEAARYFALAIDCWKTGSGTQEVLLRSLEGMGASLIKDGF
                     YAKALPFYQEVAFLDRLKYGAESIPYAWSLRVLNDIYLKLEDPITARRCFERSVWNFR
                     NANRERLLQVWTKRLANTENPPCAADLDKLLSDRIFGSLGKISDVDFLKAGAYTGVTE
                     YKLEPSLPNLKGDRTCPEHGMPWSRQRIAVQEPASMFWVDPREKVQGFVVCIPGFGLH
                     RGSFSDLGESLSSKGFVVASYDVRGFGAYTAMKARDRIDLEKSLEDLDDSIIQIRKDF
                     SGVPVFVLGESMGGSLALQFCAKHPELVEGLIAAVPSEQRYRQWLTAGKVILGMLTGS
                     RKQIDVGPVLVNRATQDEGLRQTWTSDPQSRFTATAKELYGFKRFVSRNRDLAKQVKT
                     TPVLMFQGVHDLLIRPEGTISLFKAVASEDKDLMLVGKSEHLVFEEGQFDKALLVSIC
                     DWMKTRGSK"
     gene            2577..>2808
                     /locus_tag="PKW73_08635"
     CDS             2577..>2808
                     /locus_tag="PKW73_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73391.1"
                     /translation="MALGSKELRGVWMLGQRSENRVHSFLAAGLIALSASLALPLSAL
                     AEPGLSYDHEHDVLVLPGCDLAVRYNNKTFVPV"
BASE COUNT          621 a          689 c          791 g          707 t
ORIGIN      
        1 ttttcgtatg aataaatgag gcgcattgcc accagataga cgatgatgaa aagaatgctg
       61 agcggatcta ttgagttgag ctgcttgatg gaaggaaggt atttgccaaa tagaatgtct
      121 atggcggcca gcgccagaag cacaatgccg aaaccggcgg agagttggtg tccctgctgc
      181 accatatttg atactggtct gtgcttgctg aaaaggtcta gagacgcaat gacgagcatg
      241 ttgaacatgc aactgccaag ggtaccgctt acagctagat tggggagatc gttaagagta
      301 actgctgaga ttcccgtcag cagttcaggc agcgaggtaa cagtagcaag cattaccaga
      361 ccaacccagc ttttgccgag tccagtagcg tcggcaatta catcggcatg ctttgacagc
      421 ttgctgcccg tgacaagaat aacggcagca cataaggcaa actctgcaaa tacgaaattc
      481 tgttccaatt acttgctacc tctggttttc atccagtcac agatgctgac cagaagagct
      541 ttatcgaatt gtccttcctc gaagaccagg tgctccgatt tgccgaccag catcagatcc
      601 ttgtcctcgc tggccacggc tttgaagaga ctgattgtgc cttccggtcg gattagcagg
      661 tcgtgtacac cctgaaacat aagcaccggc gtcgtcttta cttgtttagc caggtcgcga
      721 ttgcgcgaca cgaagcgttt gaatccgtag agttctttag ccgttgctgt aaaacggctc
      781 tgggggtcgg atgtccaggt ctggcgcagt ccttcatctt gggtggcgcg gttgaccaga
      841 accggtccca catcgatttg tttacggctg cccgtaagca ttcccaaaat taccttgccg
      901 gccgtcagcc attgccgata gcgctgttcg gagggcacag ctgcaataag cccctccact
      961 agctccgggt gcttggcgca gaattgcagg gccagggagc cgcccattga ttcgcccaac
     1021 acgaagaccg gcaccccgga aaagtctttg cgaatctgaa taattgagtc gtcgagatct
     1081 tcgaggctct tttccagatc gatgcgatct cttgctttca ttgccgtgta ggcgccaaag
     1141 cccctcacgt cataggaggc aactacaaaa cccttggagg agaggctttc gcccaggtcg
     1201 gaaaaggaac cgcgatgcag tccaaagcct ggaatacaga ccacaaagcc ttgtactttt
     1261 tcgcggggat cgacccagaa catgctggct ggttcttgta cggcaattcg ctgacgagac
     1321 cagggcatac catgttcagg acaagttctg tcgcccttca ggttgggaag ggatggttct
     1381 agtttgtact ctgtgacgcc ggtgtaggcc ccggccttga gaaaatccac gtcagagatc
     1441 ttaccgagcg agccgaagat gcggtcactc agcagtttgt ccagatcagc ggcacagggc
     1501 ggattttcgg tgttggccag acgtttggtc caaacttgca gaagtctctc tcgattggca
     1561 ttgcgaaagt tccaaaccga tcgctcgaag catcgccggg ctgtaatggg atcttcaagc
     1621 ttaaggtaga tgtcgttcaa aactctcagg gaccaggcgt agggaatgct ttcggctcca
     1681 tacttgaggc ggtctaaaaa ggcaacttcc tggtagaagg gaagcgcttt ggcatagaag
     1741 ccgtctttga tcaggctggc gcccattcct tccagactgc gcaatagaac ttcctgcgtg
     1801 ccggagccgg ttttccagca atcaatagcc agggcaaaat agcgggcagc ttcgtcatgc
     1861 ttatccagct cgtagagggc ttcgccgata tcgcaataag tctcggctag aagttgttct
     1921 ctatcttgat caggcaagag tatctctttg gcgctgccgg tttttccctt cctggaggta
     1981 ctcatcggtt ccgcctgaga aactgctttc aatacggtga gattgcgctt gtgcatattc
     2041 aatgctgtca ggtattcacc gcattgataa gcgctgtctg cggcagcgac ttgggctttg
     2101 attcggtcaa gcggggagag gcttttgtcc agggctgcag ccttgaaggc gtgcagggct
     2161 gctccgaagt ttccttgttc gtagtcattg aggccggttg ccaggtggtt ggcggtggtt
     2221 gacggttttt ctactttggg gctgccatgg gtctcagcca gggcctggcc gggaaatagg
     2281 gcgacagatg tgaaaactcc agctactgcc agactgaggc tgagaaagac tcgagtttta
     2341 tggtctttcc ctttgcaagg gccgttttgg cgaaggcatg attctggcaa ggcagcggtc
     2401 ctggaggggt gaagcataag ctccttgagc cggttcaaat tgttgtcgga gttagcagga
     2461 tacaagtata tagtcggatt tgctataggt tacggctatt ggcgcaagct gagcaattgt
     2521 ctacctcgat cggttaaaat accctcttta cactacgctg cagtgcggcg gcggccatgg
     2581 cgcttggaag taaagagttg cgaggtgttt ggatgttggg gcaaaggtct gagaaccgag
     2641 ttcattcttt tctggctgcg ggtttaattg ccctgagcgc cagtcttgct ctgcctcttt
     2701 ctgcgctggc cgagcccggc ctcagttatg accatgagca tgatgtgttg gttttgcccg
     2761 gctgcgattt ggccgtgcgt tataacaaca agacttttgt gcctgtca
//
LOCUS       DAOQNZ010000429         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19624_length_2806_cov_3.127953, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000429 DAOQNZ010000000
VERSION     DAOQNZ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19624_length_2806_cov_3.127953"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1517
                     /locus_tag="PKW73_08640"
     CDS             96..1517
                     /locus_tag="PKW73_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNA73392.1"
                     /translation="MKRSFDYRVRSLMLPGLKRYILVILVSICAIVFGVLWMLDKHPV
                     LRIQKFLHEVVSDTAAVLPSKVNGIIAISVGSICLLGAMVRITQTLLGAYLPEDREAI
                     PDVLYRRRYLERGPKVVVVGGGTGLSNLLNGLKRYSNNLTAIVTVGDDGGSSGRLRQE
                     LGVLPPGDIRNCITALADQDKIVTELFRYRFSSGEGLEGHSFGNLFITALCAVTGGDI
                     IQATRTASRVLNSCGQVLPSTLSSLTLTAEYTDGSKVVGESSIVEMGKRIRAITCEPA
                     QPEAVAEAVEAIMEAELIVLGPGSLYTSIIPNLLVPGIVSAIREAQARKIYICNVMTQ
                     PGETTGYSVGDHVEALLAHAGPVRTPAGRLLHSVLVNESEGGHENEVRYDPEKVKELG
                     LTVLRRPLTSATAVSHHDSTRLARAIIMWLFRTGRTRTTRVRSGTAPKGKVKQVDTKE
                     SGLLKSEGGTNSKEVASSTNATL"
     gene            1544..2026
                     /locus_tag="PKW73_08645"
     CDS             1544..2026
                     /locus_tag="PKW73_08645"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNA73393.1"
                     /translation="MTTGVEMKEKVGPALGRIASGVYIVTVGGKEDRDGMLATWICQS
                     GFAPPSVVLVVNKERDILKRLAIGANCAVNVLSKSNMDIFKAFAKPHNEGKFDGLDLV
                     EGTPCPVFSACISYMQVQIKSSSDAGDHVVLVGEVLDGAVLNGDSEPMVHLRKNGFQY
                     "
     gene            complement(2023..>2806)
                     /locus_tag="PKW73_08650"
     CDS             complement(2023..>2806)
                     /locus_tag="PKW73_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNA73394.1"
                     /translation="TSIGGINAAIYAQGDQTRLEELWCHIKSKDVFALPSAHQVGRLV
                     LGHKLGLLDTQGLEEILRREINLQKLKASKTKVGWCTTDLCSLQTKLITIDEIMSTNE
                     LIDVLMATSAIPMAFPPRHIHGKGLWIDGGLVRNTPMETAIHLGADEVYMVLLHPEQI
                     NVCPVNMFEVLVRCLDIVLDASARKEIESAQLYNRLVESGSKESIGRKQVKIKVFQPK
                     KGVNTNLLEINPELSRKLIRQGYDDAREQLALMNEVEMQVTA"
BASE COUNT          596 a          696 c          805 g          709 t
ORIGIN      
        1 agtagggtta acggtttcat cgccatttct gttggttcga cttgcctttt gggcgccatg
       61 gtgcgcctca cgcagatgct gcgcgaggct aactgatgaa gcgctccttt gactatcgcg
      121 taagaagtct gatgctgccg gggttgaagc gctacatcct ggttattctg gtcagcattt
      181 gtgccatcgt atttggtgta ctttggatgc tcgacaagca cccggtattg cgcattcaga
      241 agtttctgca cgaagtggtt tctgatacgg ctgccgtttt accgagtaag gttaacggta
      301 tcatcgccat ttcggttggt tcgatttgcc tcttgggcgc catggtgcgc atcacgcaga
      361 ctctattggg ggcctatttg cccgaagaca gagaggctat tcccgatgtt ttgtatcggc
      421 ggcgctatct ggagcgtgga cccaaggtgg tggtggttgg tggcggcacc ggcctttcca
      481 acttgctgaa cgggctgaag cgttactcga acaatctcac tgccattgtc acggtcggtg
      541 atgatggcgg cagttcaggg cgtctcaggc aggaactggg tgttttgcct ccgggtgaca
      601 taagaaactg cattaccgcg ctcgccgacc aggataagat cgtcaccgag cttttccgtt
      661 atcggttttc ttcaggtgag gggctggaag ggcatagttt cggcaatctt tttattacgg
      721 ctctttgtgc cgtcaccggc ggtgatatca tccaggctac acgtacagcc agtcgggttt
      781 tgaatagttg cgggcaggtc ttgccttcca ccctttccag cttgaccctc actgccgaat
      841 atacagacgg tagtaaggtt gtcggcgagt cttccattgt cgaaatgggt aagcgcattc
      901 gagccattac ctgtgagccg gctcagccgg aggccgtggc cgaagctgtg gaagcaatca
      961 tggaagcgga gctgatcgtg cttggtccgg gtagtctcta cacatccata attcccaatc
     1021 tgctcgtacc cggtattgtg tctgccatta gagaggccca ggcacggaag atttatatct
     1081 gcaacgtcat gacgcagccg ggtgagacaa ccggttactc cgtcggagac catgtggaag
     1141 ccctcctggc tcatgccgga ccggtgagaa cccctgccgg caggttgttg cactcggtac
     1201 tggtaaacga atccgagggc ggccacgaaa acgaagtgcg ctatgacccg gagaaggtca
     1261 aggaactggg cttgaccgtg ctgagacgtc ctttgacaag tgccacggcg gtatcccatc
     1321 acgacagtac ccgcttggca agggcgatta ttatgtggct cttccgcacc ggcagaactc
     1381 gcacgacccg ggttcggtcc ggcacggctc ccaagggcaa ggtcaaacag gttgacacga
     1441 aagagtctgg gctcttgaaa tccgagggcg gtaccaacag caaagaagtt gcctcctcaa
     1501 cgaacgctac tctataattg cctctcactt atggagaatg attatgacaa ccggcgttga
     1561 aatgaaagag aaggttggcc cagctctcgg tcgcattgcc tcaggcgtct atatcgtcac
     1621 tgtaggtgga aaggaagatc gggatggtat gttggccacc tggatctgtc agagtggatt
     1681 cgcgccacca tcggtagtgc tggtggtaaa taaagagaga gacattctca agcgtctcgc
     1741 cattggcgcc aactgtgccg tcaacgtgct ctccaagagc aatatggaca tttttaaagc
     1801 ctttgccaag cctcacaatg aaggcaaatt cgatggtctt gatttagtgg aaggcacacc
     1861 ctgtcctgtg ttttctgctt gcatcagcta tatgcaggtg cagatcaaat ctagttccga
     1921 tgccggtgat catgtggtgc tggttggtga agtgctggac ggcgccgtat tgaacggcga
     1981 cagcgagccc atggtgcacc tgcgaaagaa cggcttccag tactaagctg taacctgcat
     2041 ctcaacttcg ttcatgagcg ccagctgttc gcgggcgtcg tcataaccct gcctgattaa
     2101 cttacgggac agttccggat tgatttccaa aaggttggta ttgacgcctt ttttgggttg
     2161 aaagactttg attttgactt gcttccgtcc tatggattct ttggagcctg attccaccag
     2221 gcggttgtaa agctgggctg attcaatttc tttccgggct gaggcatcaa ggacgatgtc
     2281 caggcagcgg accagcactt cgaacatgtt gaccgggcaa acattgattt gttcggggtg
     2341 taaaaggacc atataaactt catcggcccc gagatggata gcagtttcca tgggcgtatt
     2401 gcgtaccagc ccgccgtcta tccagagccc tttaccgtga atgtggcggg ggggaaaggc
     2461 catgggaatg gctgaagtag ccataagaac gtcgattagt tcattggtgg acatgatctc
     2521 atcgatggtg atgagcttgg tctgcaagct gcacagatcg gtggtgcacc aacctacctt
     2581 ggttttgctg gcttttagtt tctgcaggtt aatttcccgg cgcaaaattt cttccagccc
     2641 ctgggtgtcc aggagaccga gtttgtgacc gagaaccagt cgcccaacct gatgggcgga
     2701 cggcagggca aagacatctt tgctcttaat gtggcaccag agttcttcca ggcgggtctg
     2761 gtctccctga gcgtaaatgg ccgcattgat gccgccgata ctggtt
//
LOCUS       DAOQNZ010000430         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19642_length_2804_cov_3.526737, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000430 DAOQNZ010000000
VERSION     DAOQNZ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19642_length_2804_cov_3.526737"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKW73_08655"
     CDS             <1..704
                     /locus_tag="PKW73_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNA73395.1"
                     /translation="SEAARLLRYERQLGLPGFSQTQQEKLFMGSVLLLGASFVSRTVA
                     MSLAQSGVGTIKLASASLSELSSARQFLSSVETPDSKLSFQLLSEPLMGHLEELMVAY
                     DVIVDGVERWQTKLLASDVAMRLGKPLVHSHSSGLRCQVYCMVPGRSMCLRCLLIQLE
                     MEDFPREEASRFSSLPALESIVGGFQALEVIKLLSGFGVSQGNELMRFDGLSGELEVL
                     RGLDPKSDCPDCGRP"
     gene            complement(701..1501)
                     /locus_tag="PKW73_08660"
     CDS             complement(701..1501)
                     /locus_tag="PKW73_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73396.1"
                     /translation="MKKAKAFTSRGHSQKIYSISRDGLLSVFGTGLALVLSLVCIKEA
                     QAFALKDKDGRSIHGSITREALGNKFSSKNLAVLIESGDQTLPSTGQFFFSKGKLKSS
                     LSFLDREQKKIINYCHDADSNPKDRYRALKHLGALLRASQDFYSHSKYLELESERLKN
                     RKDKTFDPYQIDLVDWSRVTDPDYKALNGSTIDDGGRPMETEEEGAEKLGGTTYFRAA
                     RELAVRETARQWDMLSTLIKRKYKDKSLTILTALREASAPPVDDDDVN"
     gene            1663..2529
                     /locus_tag="PKW73_08665"
     CDS             1663..2529
                     /locus_tag="PKW73_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNA73397.1"
                     /translation="MSLAAVNAQAGIKGPLKELQFVAFDVETTGLSAIACRLVELSGV
                     KFDVWHRDVSTFSSLINPGSPIPPEVSKIHGITDDMVKDAPKAEQVLRDFLHFARGGV
                     WLAHNAPFDVEFLKIELLRQKQPLPEVMVLDTLTLAQVVLSGPENFKLKTLAEYFGFG
                     GAEYHRALADSVYVEKLFGKLLEVSHFTTVEELNGTGALKSFSDWGRPASREHLPESV
                     KSHICLLEAAIESSSTVKLKYQGEFLSTRTVQPEAVIESRGSLYLSAYCLKSRAQRTF
                     RVDRIVEAIINT"
     gene            2543..>2804
                     /locus_tag="PKW73_08670"
     CDS             2543..>2804
                     /locus_tag="PKW73_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73398.1"
                     /translation="MFLAGAVLLSPWLNPFPSESAPFFFRKRSYKLDEKAIQQESEKV
                     ERDLSGSDDEKGKSGRKPEGENPAGREPLDDALALEEGEDKEA"
BASE COUNT          559 a          715 c          782 g          748 t
ORIGIN      
        1 gtagtgaagc ggcgcgctta cttcgctacg aaagacagct tggtcttcct ggtttcagtc
       61 agactcagca agagaagctc tttatgggtt ccgtgctttt gctcggggcc tccttcgtat
      121 ctcgcaccgt ggctatgagt cttgctcaga gcggtgtggg aactatcaag ctggcctccg
      181 cctcactttc tgaattgagc agcgccaggc agtttctctc ttccgtggaa acccctgatt
      241 ccaagctcag ttttcaattg ctttccgaac ctctgatggg gcacctggaa gaattgatgg
      301 tggcttacga tgtaatcgtg gacggagtgg agcgctggca aaccaaacta ttggcttccg
      361 atgtggccat gcgtctcggg aagccgcttg tgcacagcca ttcatcaggt ctgcgctgtc
      421 aggtctattg catggtgccc ggtcgctcca tgtgcttgcg ttgtttgctc attcaactgg
      481 aaatggagga ttttccccgg gaagaagctt cccggttttc ctccttgcct gcgctagaga
      541 gcattgtggg gggctttcag gccctggaag tgataaagct gctctcgggt ttcggcgtca
      601 gtcagggcaa tgaactgatg cgctttgatg ggctgagcgg cgagttggaa gtgctgcgcg
      661 gattggatcc taagtcagat tgccccgact gcggcagacc ttagttgacg tcatcgtcat
      721 ccacgggcgg agcgctggcc tcccgtaagg ctgtcagaat tgtcagcgac ttgtccttat
      781 acttgcgctt aatgagggtg gagagcatat cccactgcct cgccgtttct ctcaccgcca
      841 gttcgcgggc ggctctgaaa taggttgtgc cgcccagttt ttcggcgccc tcttcttcgg
      901 tttccatggg tctgccgcca tcatcgatgg tgctgccgtt cagtgcctta tagtcggggt
      961 cggtgaccct gctccagtcc accaggtcga tttggtaggg atcgaaggtt ttgtccttgc
     1021 ggtttttgag gcgttccgat tccagttcca gatattttga gtgactgtag aagtcctggc
     1081 tggccctcaa tagggctccc aggtgcttga gggcgcggta ccgatctttg ggattgctgt
     1141 ctgcatcatg gcagtagttg atgattttct tttgctctct atcaagaaag ctgagggaac
     1201 tcttcaattt gccttttgaa aaaaagaact gcccggtgga aggcagtgtc tgatcgccgc
     1261 tttcaatcaa gactgccaga ttcttcgagg aaaacttatt gcccagggct tctctggtaa
     1321 tggacccatg gatggagcgc ccatctttgt ctttgagcgc gaaggcctgt gcttccttga
     1381 tgcataccag gctcagtacc aatgccagac cggtgccgaa gaccgaaagc aagccgtccc
     1441 ttgagatgct gtatatcttt tggctatgtc ctctgctcgt gaaggctttt gcttttttca
     1501 tttttccgcg tctttgccgg taaattgcat tattcatcac tatagtgcca atattttccc
     1561 tctgcccgaa cctttcaaga attactctta catacaacca atttccgctt tggcgctttg
     1621 ctgtatggta gctctgtatt tttgaaattt aaggaaggca agatgagttt agcggccgta
     1681 aacgcccagg ctggcattaa aggtcctctc aaggagcttc agttcgttgc ctttgacgtg
     1741 gaaaccaccg gcttgtcggc tattgcctgt cgcctggtgg aactttccgg tgttaagttc
     1801 gatgtctggc atcgggatgt ctccaccttc agctccctta tcaatcccgg ctcgccgatt
     1861 ccgcctgaag tttccaaaat tcacggcatc accgatgaca tggttaagga tgcgccaaaa
     1921 gccgagcagg ttctgagaga cttccttcac tttgccaggg gcggagtctg gctggctcac
     1981 aacgcgccct ttgacgtgga gttcttgaaa atcgaactct tgcgccagaa gcagcctctt
     2041 ccggaggtga tggttttgga cacgctcact ctggcccagg tcgttttaag tggaccggaa
     2101 aacttcaagc tcaaaaccct ggctgaatat tttggcttcg gcggcgctga gtatcaccgc
     2161 gccctggccg atagcgtcta tgtggagaag ctcttcggta agcttttgga agtttctcac
     2221 ttcaccaccg tggaagaact gaacggcacc ggagcgctca agagctttag tgactggggg
     2281 cgtcccgcca gccgagagca tttgcccgag agtgtcaaaa gccatatctg ccttctggag
     2341 gcggctattg aaagcagtag cacggtcaag ctcaagtatc agggtgagtt tctcagcacc
     2401 cgcacggttc agccggaagc cgtcattgaa agccgcggca gtttatactt aagcgcatac
     2461 tgcctgaagt cgagagccca gagaaccttc cgtgtcgata gaattgttga ggcgatcatt
     2521 aatacctaga gccggtttcg gcgtgtttct ggctggggcg gtactgcttt cgccctggct
     2581 caatccgttc ccgtctgagt cggccccttt cttctttcgt aagcgttctt acaagcttga
     2641 tgagaaggct atacagcaag aaagcgaaaa ggtcgagcgc gatttgagcg gtagcgatga
     2701 cgagaaaggt aaatcggggc ggaagccgga gggcgagaat ccggccggtc gagagccact
     2761 tgatgatgcc ctggctctgg aagagggcga ggacaaggaa gcgg
//
LOCUS       DAOQNZ010000431         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19652_length_2803_cov_3.886463, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000431 DAOQNZ010000000
VERSION     DAOQNZ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19652_length_2803_cov_3.886463"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1259
                     /locus_tag="PKW73_08675"
     CDS             75..1259
                     /locus_tag="PKW73_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA73399.1"
                     /translation="MFRLFTFPLLLAAFGLSGGAAYVQDMFAGMGSLPSIIGMILGLC
                     LLITIHELGHWLVARLCGMQTPVFSIGFGKREWSLVLGKFWETEFRIAPILAGGYVSI
                     PELLDETSLNEAAKDGELPKNLRVFPVWQRIAVAAAGVTFNMISAVLMVFVLFTFWGQ
                     PDYKVNSTYIRDLSTQVTVARDAGLQPKDQFVSVDGRQVVSPDDLSAALKANKEKPVQ
                     VVVKRGSGDVTVTVTPTKDGTIGIVLGANAERIYKEMSVGDAAVNSVKVTGKVLDMTF
                     KGFGMMLGLVEKPANISDADMEVRGIVGIVQMGAGALGTGAFDFIWFLMVISINLAVM
                     NILPIPVLDGGHIVFFLIEGVTGKPVNPRTKALLSNIFVMLLLALFALGLFNDLKHIV
                     GG"
     gene            1315..1608
                     /locus_tag="PKW73_08680"
     CDS             1315..1608
                     /locus_tag="PKW73_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73400.1"
                     /translation="MLLLISLALAALAFSCGAYVGKGKALGGMQVVILWSWVVLLTPS
                     TAYLYWVGGPAVILAVFLLFCLAEILALLLFCSRDNSPSKGEKELLERLQKRD"
     gene            1876..2133
                     /locus_tag="PKW73_08685"
     CDS             1876..2133
                     /locus_tag="PKW73_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNA73401.1"
                     /translation="MEKVNIHQAKTQLSRLIEQVLRGEPFIIARAGKPLVKVTRIDAP
                     AAGQSRRLGFLTGQFTVPDDFDRMGSDEIESSFGFSGESAE"
     gene            2137..2526
                     /locus_tag="PKW73_08690"
     CDS             2137..2526
                     /locus_tag="PKW73_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA73402.1"
                     /translation="MKILLDTHLLLWAAAAPERLAPATRSMLEDPSTELLFSAASLWE
                     VAIKQGLGRADFQADARLLRRGLIDNGYLELAISGAHAVFTSSLPPIHKDPFDRILVA
                     QATVEGIVLLTTDEIVARYPGPVRKVE"
BASE COUNT          553 a          751 c          791 g          708 t
ORIGIN      
        1 ctgcgcccgt ctttcaacca aaacacataa ccaagtaggt cgagctgaag agttcgacct
       61 gcggaggatt tactatgttc agactcttca catttcctct gctcctggcc gccttcggac
      121 tttccggcgg cgctgcttat gtccaggata tgttcgccgg gatgggtagc ttgccttcca
      181 tcatcggcat gattctcggg ctctgcctgc tcatcacaat tcacgagctg ggtcactggc
      241 tcgtggcgcg tctttgcggc atgcagacgc cggttttctc aatcggtttc ggcaagcgcg
      301 aatggtcgct ggtgctgggc aagttctggg agacggagtt ccgtatcgct cccatcctcg
      361 ccggtggtta cgtctccatt cccgagctgc tcgacgagac cagcttgaat gaagcggcca
      421 aggacggcga gctgcccaag aacctgcgcg tctttccggt ctggcagcgc attgccgttg
      481 ccgccgccgg tgtgacgttc aacatgattt ccgcggtatt gatggtcttc gtgctgttca
      541 ccttctgggg gcagccggac tacaaggtga actccaccta tatccgcgac ctttccaccc
      601 aggtaacggt ggctcgggat gccggtttgc agcccaagga tcagttcgtt tcggtggacg
      661 gccgccaggt tgtctctccg gacgacctct ccgctgctct caaggccaac aaggagaagc
      721 ccgttcaggt tgtggtgaag cgtggtagcg gcgacgttac cgtcaccgtc acccccacca
      781 aggacggcac cattggtatc gtgcttggtg ccaatgccga gcgcatctac aaggagatga
      841 gtgtcggtga tgctgccgtc aattccgtca aggtgaccgg caaagtgctc gacatgacct
      901 tcaagggctt cggcatgatg cttggtctgg tggagaagcc ggccaatatc tccgacgccg
      961 acatggaagt gcgcggcatc gtcggcattg tgcagatggg tgctggtgct ctcggcaccg
     1021 gtgcttttga cttcatctgg ttcctgatgg tcatttccat caacctggcg gtcatgaaca
     1081 tcctgcccat cccggtgctg gacggcggac acatcgtctt cttcctgatc gagggtgtga
     1141 ccggcaagcc cgtcaatccg cgcaccaagg cactgctctc caacatcttc gtgatgttgc
     1201 ttctggcgct cttcgcgctc ggtctcttca atgacctgaa gcacatcgtc ggcggctgag
     1261 gagcttgctt cacggtgcca acacccaacc ccaactagag gagaacacat attgatgctc
     1321 ctgctaatca gcctggcact ggctgcgctg gcgttttcgt gcggcgcata cgtaggcaag
     1381 ggtaaagccc ttggcggcat gcaggtagtg atactctgga gctgggtggt ccttctgacc
     1441 cccagcacgg cgtatctcta ttgggtgggc ggaccggcgg tcattctggc tgtctttctg
     1501 cttttctgtc ttgccgaaat tctggcgctc cttctctttt gctcgcggga caattccccg
     1561 agcaaaggtg aaaaggaact gctggagcgt ctgcaaaagc gcgactagcg ccggcgcgga
     1621 cgtgaaatca taacaaaaca agcgttttgg gcagggtgtg ttcttcaagt tccctatggg
     1681 agcttgtttg aacgcaccct gctttttttg attggaagta atagaccggt cgactacatt
     1741 ggcgcaaaag aaaaagctgg gctgcccttg cgttcgtgag aggcgtagct ctgctgcaca
     1801 ggcactgaat cccctcggtt ggtggtggcc cttctgtgat aaactaagct aagtaaacta
     1861 agggcgaaaa tgacaatgga aaaggtcaat attcaccagg ccaaaacaca actttctcgt
     1921 ttaattgagc aagtattgcg tggcgaaccg ttcatcattg ccagagctgg aaagccgctg
     1981 gtgaaagtaa ctcgcattga tgcgcccgct gccgggcaga gccgccgcct gggctttttg
     2041 accggacagt tcactgttcc tgatgatttc gatcgtatgg gtagtgatga gatcgagtct
     2101 agttttggct tctctggaga aagtgcggaa tagttagtga agattttgct cgatactcac
     2161 ttgcttctct gggctgctgc cgcaccggaa cgactagcgc ctgcaactcg ttccatgctg
     2221 gaggatccat cgacggaatt gttgttcagc gccgccagtc tctgggaagt ggcaatcaaa
     2281 caaggcctcg gtcgagccga ctttcaggct gatgccagac tcttgcgacg ggggctgata
     2341 gacaacggtt atcttgagct ggcaatttct ggtgctcatg ctgtatttac ttcgagcctg
     2401 ccgccgattc ataaagatcc ctttgatcgc atcctcgtgg ctcaggctac tgttgaaggt
     2461 attgttcttt tgactaccga cgagattgtg gcacgttatc cgggtccggt caggaaagtg
     2521 gagtaggagt acctttccgg ctccttttcg ctgagctggt gttcccttat ttgctttttt
     2581 gcagcggaag aagagaccgg tctattattg ttgttgaaaa aaatcagctt ccttgggcat
     2641 ttaagtgaga tggtgcctgc gccgaactcc gttcagccca caggcaaatt aatgcaacag
     2701 tgccatccgg gcactgtaat ttagccaact tccatccgcg ctagaggcgg atggttaggt
     2761 ttgaatcaag ccttcctgtt gtgagcgcct ctggaagaga tac
//
LOCUS       DAOQNZ010000432         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19733_length_2797_cov_3.150255, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000432 DAOQNZ010000000
VERSION     DAOQNZ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19733_length_2797_cov_3.150255"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PKW73_08695"
     CDS             complement(<1..508)
                     /locus_tag="PKW73_08695"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA73403.1"
                     /translation="MDLYDITIIGGGPTGLFGSFYAGLRGLKVKVIDVLPELGGQLTA
                     LYPEKYVYDVAGHPKILAKDLAKNLATQASLFKPTIVLGEKVLDLKKGEDGNWQIVTD
                     QADNHYSKTVLLALGAGACVPKKLDADYPRELEGECIFYAVKNKERFRGKNVLIIGGG
                     DSAVDWANE"
     gene            624..1613
                     /locus_tag="PKW73_08700"
     CDS             624..1613
                     /locus_tag="PKW73_08700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA73404.1"
                     /translation="MASHKMKYRDFGKTGIKVSEIGFGTWAIGGNSHGMSYGPTDDKA
                     STDAVMRAMDLGLNFFDTADVYGWGHAEELLGKCFRGRRDRIILAAKVGADFYQGTGF
                     QTFTPEYIRFALEKSLARLQTDYLDVYQLHNPPLKLLGNEATYATLKELKKEGKIRAW
                     GVSVFTAVEGITAINVGHPDCLQIAYNIFSFRPAEQLFPLAFEKGCAIIAREPLANGF
                     LTGKYKSGKPFRFAAGDMRKSWPDDYIKARIEAAAQLGFLERVGQSLPQAALRYSLSE
                     ASVSTVIVGIKTPEQADENIEAVQAGLKETELAKIREMQARGFKPEREIWKES"
     gene            complement(1629..2471)
                     /locus_tag="PKW73_08705"
     CDS             complement(1629..2471)
                     /locus_tag="PKW73_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73405.1"
                     /translation="MSSEVVSLSGVFMDGGGADGGVDHGHDHGHDHGHHHHQHGHNSV
                     AQFLGLENDHDHGDHDHGEAPQQTAAWNSALKGLSLKSLTQGLVITPNFLFLMLFLAF
                     TGWLGVVYWVRHNEPFANAVLGSGRAYAPTAHADRRLLDGVREAMPFKTSPNTGKIYT
                     PTPGPGPQPYGYQVPQAAAIGSIASPGLAPAQIPSAPALGHFAAPHSMRSYGNVPQYN
                     PAPAPAPAAGYGWSLAEGGFSQESAHFMSQFGAPQALSPAYSHVYVPVATPTGQRLKV
                     YTTR"
     gene            complement(2594..>2797)
                     /locus_tag="PKW73_08710"
     CDS             complement(2594..>2797)
                     /locus_tag="PKW73_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73406.1"
                     /translation="KIASQLIENQRQLVATQQILIKLMERSIELTRHITSIEERMPAL
                     LEVPRVVESLRQRVAQIEGVEVD"
BASE COUNT          679 a          760 c          781 g          577 t
ORIGIN      
        1 attcattggc ccaatcaact gcggagtcgc cgccgccgat gataagcaca ttcttaccgc
       61 ggaatctttc tttgttcttc acggcataga agatgcattc gccttccagt tcacgcggat
      121 aatcggcatc aagcttcttg ggtacgcagg caccggcacc cagggccagc agcacggttt
      181 ttgaatagtg attgtcagcc tggtctgtaa cgatctgcca gttgccgtcc tcacccttct
      241 tcaggtccag cactttctcg ccgagcacaa tcgtgggctt gaagagagag gcttgtgtag
      301 ccaggttctt agccagatcc ttggccagga tcttggggtg accggcgacg tcataaacgt
      361 acttctccgg gtaaagcgcg gtgagctgcc caccaagttc gggcagaacg tcaatcacct
      421 ttaccttcag gcctctaaga ccggcataga aagaaccaaa taaaccagtc ggcccgccgc
      481 cgataatcgt aatgtcgtat agatccatga gtgaatccaa gtctccagca taatctaata
      541 atcaaggggc tattgtaata cgaagtatcc cctcaaggcg ttgctattca cgaataatga
      601 aggagctgaa acaccttgcc tagttggcct cccacaagat gaagtatcga gacttcggca
      661 agaccggtat caaagtgtcc gaaatcggct tcggtacctg ggctatcggc ggtaactcgc
      721 atggcatgag ttacggtccc accgatgaca aagcttccac cgacgctgtc atgagagcca
      781 tggatctggg cttaaatttc ttcgacactg ccgacgtgta cggctggggt catgccgaag
      841 agctgctcgg caaatgcttc cgaggcaggc gagatcgcat catcctggca gccaaagtag
      901 gtgccgactt ctatcaagga acgggctttc agaccttcac ccccgagtac attcgctttg
      961 ctctggaaaa gagcctggcc aggctacaga cagactatct tgatgtctac cagctacata
     1021 atccgcccct caaactgctt ggcaacgagg ctacctatgc caccctcaag gagcttaaga
     1081 aagaaggcaa gataagagcc tggggtgtct ccgtcttcac ggccgtagag ggaataacgg
     1141 caataaacgt gggtcacccc gattgcctgc agattgctta caacattttt agtttccgtc
     1201 cggcggaaca gttatttcct ctggcctttg aaaaaggctg cgccataatc gccagggaac
     1261 cactggccaa tggcttcctc accggcaaat ataaatcagg taagcctttt cgctttgcgg
     1321 ccggcgacat gcgcaaaagc tggcccgatg attacataaa ggctcgtatt gaggccgcgg
     1381 ctcaattggg cttcctggag agggtcggac agagcctgcc ccaggcggcg ctgcgataca
     1441 gcctcagcga agcttcggtc tccacggtaa tagtaggtat aaaaacaccg gaacaagccg
     1501 atgaaaatat agaagcggtg caggccggtc tcaaggagac ggagctcgct aaaatcagag
     1561 aaatgcaagc caggggcttc aaaccggaaa gagaaatctg gaaagaaagc tagagcccgg
     1621 gcaggcggtc agcgagtggt gtagaccttc aggcgctgac cggtgggcgt tgccaccgga
     1681 acatagacat gagaataggc gggagaaaga gcctgtggcg caccgaactg actcatgaaa
     1741 tgcgcactct cctgagaaaa tccgccttca gccagggacc atccatagcc tgccgccggt
     1801 gccggagcgg gtgcgggatt atactgcggc acattgccgt aagagcgcat ggaatgcgga
     1861 gcagcaaagt gtcccagggc gggggcagag ggaatctgcg cgggagccag gccggggctg
     1921 gcaattgacc cgatggcggc tgcctggggc acctgatagc cgtaaggctg cgggcccggt
     1981 ccaggagtgg gcgtgtaaat cttgccggtg ttgggggaag tcttgaaggg catcgcttcc
     2041 cgcaccccat ccagaaggcg gcggtcggca tgggctgtgg gcgcataggc ccgaccgctg
     2101 cccaaaacgg cattagcaaa gggctcatta tgacggaccc agtaaaccac tcccagccag
     2161 cctgtgaaag ccagaaagag catgaggaag aggaaattgg gagtaatgac cagcccctgg
     2221 gtgagggatt tgagactcaa tcccttcagg gcgctgttcc aggctgcagt ctgctgcggc
     2281 gcttcaccat ggtcatggtc gccgtgatcg tgatcgtttt caagacccag aaattgagcc
     2341 accgaattgt gaccatgctg gtgatgatgg tgaccatggt cgtggccgtg atcatgacca
     2401 tgatccacac ccccgtccgc accgccgcca tccatgaaaa cgccgctcaa ggaaactacc
     2461 tcggaagaca cccctacatc aggtgattct aggcagttgc ccggcgaagt aaatggtaaa
     2521 accacgcata ggacgccagc ttttaaaaat tttagcttcg gtaaagatgg aagagagccg
     2581 cgcccggcct ggtctagtct acttccacgc cttcaatttg agcgacgcgc tgccgcagac
     2641 tctcgaccac ccgcggcacc tccagaaggg ccggcatgcg ctcttcgata ctggtaatgt
     2701 gcctggtcaa ttcgatggaa cgctccatca gcttaatcag gatttgctgg gttgctacta
     2761 actgccgctg attctctatt agttggctgg caatttt
//
LOCUS       DAOQNZ010000433         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19763_length_2795_cov_3.771898, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000433 DAOQNZ010000000
VERSION     DAOQNZ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19763_length_2795_cov_3.771898"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKW73_08715"
     CDS             complement(<1..275)
                     /locus_tag="PKW73_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73407.1"
                     /translation="MSTSTAENQNSTASTAEKLSGQAVENKPLSTQPERETRTDKSSG
                     RGKLRFALEMTALLPLSILALEALLGFMGVGQQEIVRPDPELGCVHLP"
     gene            complement(287..1792)
                     /locus_tag="PKW73_08720"
     CDS             complement(287..1792)
                     /locus_tag="PKW73_08720"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA73408.1"
                     /translation="MLFNSFSYLAFFLLVFTLYWVMPDKHRRLFLLLASYFFYMSWKP
                     EYGLLILALTVVNYWLGLKIGNLLSEQPQKAKNWLSVGLVFNLGCLCFYKYTNFLLQS
                     FFSAITYGGSIAHIKALETAQSPAINIILPLGISFFTFEFIHYIVDVYRGSKPVKSPL
                     DFALFASFFPSQIAGPIKRFQDFMHQLEEKPTLKAEYFNEGLFLILQGFFKKIVLGDN
                     IGLLVGQGFHNLQNLSLCDAWVTAIGFTFQIFFDFSGYTDIGRGSALLLGYKVPENFN
                     LPFLAHNLTNFWHRWHISLSTWLRDYLFIPLGGSRGSAWQTRRNTFLTMVLGGLWHGA
                     DWTFVIWGILHGAGLILSKEWTDFAARTSWLKPLRQTRVWHWCGVAFVCLFICQVSIF
                     MRAPNLDQALCLVGKFFGFGINGITGDVSTALLQSTVVYTLPVYLLYAWLHQRSLTMA
                     KNGVALPSLARLAQSCWQSSKALRLTTSAMLALFISCMAPSQLSPFIYFQF"
     gene            complement(2011..>2795)
                     /locus_tag="PKW73_08725"
     CDS             complement(2011..>2795)
                     /locus_tag="PKW73_08725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNA73409.1"
                     /translation="LERAGIKLNTRLTRNHASLSEQEFEAAVSPLVPLDLDMQKRFYQ
                     LIDNKSALLEILDKGKFNRCHWIPALYNLMSGSLVTAEEEMVEHQNTTGNLTIGIDRS
                     TVEQFAKSHIYSDTGIVTFVALLYFIEQEFYRYECFQSPFSLVMIKPRIVRGGQDFPI
                     SQEQRAAVLAGLSATKRKADLLAHFQQDDFALLLPQTTPEGVRSFAGRLVEALKPGPQ
                     PVGINLQPGEIFSMSVGIASLPDDCQDWVKLVAFLNQNQRRF"
BASE COUNT          685 a          658 c          842 g          610 t
ORIGIN      
        1 ggcagatgaa cgcaacccag ctcaggatca ggtctgacga tttcttgctg gccgacgccc
       61 atgaaaccaa gcagagcttc cagtgccaga atggaaagag gcagaagggc tgtcatttcc
      121 agggcaaagc gcagcttgcc gcgtccggaa cttttatccg tccgtgtttc tctttcgggc
      181 tgcgtgctca gtggcttgtt ctcaacggct tgcccgctga gcttttctgc agtgctggcg
      241 gtgctatttt gattttcagc tgtgctcgta ctcatttttt aagcacctaa aactggaagt
      301 aaatgaaggg ggaaagctga ctgggtgcca tgcacgatat gaaaagagcc agcatggcgc
      361 tggttgtcag tctcagggct ttagatgact gccagcagct ttgggccagg cgggctaggg
      421 acggcagtgc cacaccgttt ttggccatgg tgagggagcg ctggtgcagc caggcataga
      481 gcaagtaaac cggcagcgtg tagacaaccg tggattgcaa aagggcagtg gaaacatcgc
      541 cggtgatacc gtttatgccg aaaccaaaga acttacctac cagacagaga gcctgatcga
      601 gattgggtgc ccgcataaat atggaaacct ggcagatgaa cagacaaacg aaggcgacgc
      661 cgcaccagtg ccagaccctg gtctgacgca ggggtttgag ccagcttgtg cgggcggcaa
      721 aatccgtcca ttctttggaa aggatgagac cggcgccgtg caggatgccc cagatcacaa
      781 aggtccagtc ggcaccgtgc cagagaccgc ccagaaccat ggtcaaaaag gtgttgcgcc
      841 tggtttgcca ggctgaacca cgggagcctc ccagcggaat gaagagatag tctctcagcc
      901 aggtggaaag cgaaatgtgc cagcggtgcc agaagtttgt caggttgtga gccaggaagg
      961 gcagattgaa gttttccgga accttgtagc cgagcaagag ggctgagcct ctgccgatgt
     1021 cggtataacc ggagaagtca aagaaaatct ggaaggtgaa accgatggcc gtcacccagg
     1081 catcacaaag ggagaggttt tgcagattgt gaaagccctg cccgaccaga agaccgatgt
     1141 tatcgcccag aacgatcttc ttgaagaagc cctgcaaaat gagaaagaga ccttcgttga
     1201 agtattccgc tttcagggtg ggcttttctt caagctggtg catgaagtct tgaaagcgct
     1261 tgatcggacc ggcaatctgc gaggggaaga aagaggcaaa gagcgcgaaa tccagtgggc
     1321 ttttgaccgg cttgctgccg cggtaaacat ccactatgta gtggatgaat tcaaaagtga
     1381 agaaggaaat gcccagaggc aaaatgatat tgatagccgg gctttgagcc gtctccaggg
     1441 ctttgatgtg ggcgatgctg ccaccatagg taatggcgga gaaaaaagac tgtaacaaaa
     1501 agtttgtgta cttgtagaaa caaaggcaac ccaggttgaa gaccagacct acgctgagcc
     1561 agttcttagc cttctgtggt tgttccgaga gtagattgcc aatcttgaga ccgagccagt
     1621 agttgaccac ggtcagggcc aggattaaaa gcccgtactc gggcttccag ctcatataga
     1681 aaaagtagct ggctagaagc aggaagagtc tgcggtgctt gtccggcatt acccagtaaa
     1741 gggtgaaaac cagcaggaag aaggccaggt aagaaaaact gttgaatagc atcgggtatc
     1801 tgcagtgggt tcctgggtag agtcctgggt aaattcccgg gtagattcca agcgatgcta
     1861 ccatgccctc caaaggggct acattgatgt aatctaattt acttatatag gcaattcaac
     1921 gccgctccta cggggtcagg tcggacccat cagcaaagga actcaggcgg agaaatcgtg
     1981 aaagaggtct ttcccggcgc ggttcgcccg ttagaaacgg cgttggttct ggttcaggaa
     2041 cgccactagt tttacccagt cctggcagtc gtccggcaag ctggcaatgc ccacgctcat
     2101 agagaagatc tctccgggct gcaggttgat acccaccggt tgggggccgg gcttgagggc
     2161 ctccaccagg cgcccggcaa aggaccgcac accttcggga gtggtttggg gaagtagcag
     2221 ggcaaagtcg tcttgctgga agtgagcgag caggtcggct ttgcgttttg tggcggaaag
     2281 cccggccagc accgccgccc gctgttcctg gctgatgggg aaatcttgac cgcctcttac
     2341 tatgcggggt ttgatcatga ccagggaaaa ggggctctgg aagcattcgt agcggtaaaa
     2401 ctcttgctcg atgaagtaga gcaaggctac gaaggtaacg atgcctgtgt cggagtaaat
     2461 gtggcttttg gcaaattgtt ctacggtgct tctgtcgatg ccgatggtga ggttgccggt
     2521 ggtgttctgg tgctccacca tctcttcctc ggctgtgacc agggagccgc tcatcaaatt
     2581 gtagagggcc ggaatccaat gacagcggtt gaatttgcct ttgtcgagaa tctctaaaag
     2641 ggcgcttttg ttgtcgataa gctggtagaa gcgcttctgc atatctaaat ctagtggtac
     2701 cagaggtgat accgccgctt cgaattcctg ctcgctcagg ctggcgtggt tgcgggtgag
     2761 tctggtatta agtttgatac cagcccgttc cagaa
//
LOCUS       DAOQNZ010000434         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19767_length_2795_cov_3.082482, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000434 DAOQNZ010000000
VERSION     DAOQNZ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19767_length_2795_cov_3.082482"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKW73_08730"
     CDS             <1..624
                     /locus_tag="PKW73_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73410.1"
                     /translation="EPEMSNEAFGTASIKLTGSYQGKPGVAYYLACTKATIGYGTGMW
                     WITLLAGEMSPAEYDQEGLAAITHMLSTFKYDPQWKANSVATAGEVSRQYTAASQAAS
                     RAITQNYWSQQAANERIHSAYWNRQAANDRSNAAYWDRQASQDASADNFSNYIRGVEN
                     VTDTATGSTYQVEYGPRYHWVDPSGSYYTGTDHSAPAPDWRQLMPTR"
     gene            complement(629..835)
                     /locus_tag="PKW73_08735"
     CDS             complement(629..835)
                     /locus_tag="PKW73_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73411.1"
                     /translation="MIDISKGCAGGAPEKGEAELFSSETAAAFIRFVQESWAESKESF
                     SKRLNFDVAEDSLEITGLAGSDKK"
     gene            complement(1531..>2795)
                     /locus_tag="PKW73_08740"
     CDS             complement(1531..>2795)
                     /locus_tag="PKW73_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA73412.1"
                     /translation="LIAGGVTNMLLTLVLGLILTRNIVSRLNVINDNSYRLASDRPLN
                     PPLAGDDEIARVDGIFHQMAGHLKNAAGREKAILESAYDCICSLDSDLRFTSANAASL
                     QFFAVPDDEIVSTKLLDYLDKDCAQSVLAFRDKARQGEMQGALKLSIRRADGEVRDVL
                     WSARWAAEEGKSGELFSIFHDVTEQSRAEQLRQEVVAMVTHDLRTPLMTVQSFLELLS
                     QGFYGEKSEVGPKEDKSHRHLAGARRSCNRMVLLIRDLLDIEKIKSGKMEMNLSNVSA
                     REVLEAAAEEATSFAAEMGVSLKVKPTKSVVRADEEMLQRVISNLVSNAIKFSASGGT
                     VTLGAHNSGQTVTFSVTDEGAGIEPAMLKNVFDRFQQARNQTSRSRGGSGLGLTICKE
                     IVQLHGGKIWVESTLGEGSQFFFEMPKG"
BASE COUNT          686 a          835 c          653 g          621 t
ORIGIN      
        1 gaacccgaaa tgtcaaacga agcgtttgga accgcttcca tcaaactgac cggcagctac
       61 cagggtaagc caggcgtcgc ctattatttg gcctgcacca aagccaccat cggctacggt
      121 acgggcatgt ggtggatcac cttattggcg ggcgagatga gtccggccga atacgaccag
      181 gaaggactag ccgctatcac gcacatgctc tccacattca aatatgatcc tcagtggaag
      241 gccaattccg tcgccaccgc cggtgaggtc tcacggcagt acacagccgc cagccaggct
      301 gcttcccgag ccattactca aaattactgg tcgcagcagg ccgccaacga acgcattcac
      361 agcgcttact ggaacaggca agcggcaaac gacagaagca acgccgccta ctgggacagg
      421 caagcctctc aagacgcctc tgccgacaac ttttccaatt acatacgggg cgtcgaaaac
      481 gtcaccgata cagccacagg ctctacctat caagttgaat acggtccgcg ctaccactgg
      541 gtagatccct ccggcagcta ctacaccggc accgaccaca gcgccccggc tccggactgg
      601 cgacagctca tgcccaccag ataggtactt acttcttatc agaaccggcc aggccagtta
      661 tctctagaga atcttcggcc acatcaaagt tcagccgctt cgagaagctc tctttagatt
      721 cagcccagga ctcttgcaca aagcgaatga atgcagcagc agtttcactg gaaaacaact
      781 ccgcctctcc tttctccgga gcaccaccgg cacagccctt agaaatatca atcatccagg
      841 tatcctcgaa tacgaacctg gtttgagagt acaccccatc tgtgtcgaaa catggtcaaa
      901 gccgacggga aatacgaatt ttgcccaata tgagaacgga cgaattgaat agcagaccaa
      961 tctgagccca ctttgaagcg gcgcacatac cctcctaccc cttccgagcc ccctttgaag
     1021 cggcgcacgg gggttcctgc ccatcgcgag ccaaaagaga gcccctgggg gtctcttcaa
     1081 agaaaaccct gctttgtaac agcgattccg cagagtggtt gccggctcag gacgtcgatc
     1141 cggtatctgg tatctggtat ctgaagtgaa tatctacagt ctagaactcc cgctacaaag
     1201 tgccgaaacc tctgtcggag acggcttggg tattcacttt gtggaaccac tgcaaatgta
     1261 acaatagtga ttttgcgaaa aattacatca gcgttttggg actttttgga aaatgaaacc
     1321 cttgctgcaa ctacttttcc gccgtcaagc agaattaatt ttgactgtta catgagttta
     1381 gccttgtcgc caccattttt taaaagacat ggtctcgtgc tgctctctgc gttttccgat
     1441 aaccgccctc tgcgtttttc gacaaccacg cgattacccc tgtgacagtt cacccatccc
     1501 gcaccgcacg gctccgcacc gcaagatagt tcaccctttc ggcatctcaa agaaaaactg
     1561 cgatccttcg ccaagagttg attccaccca gatctttcca ccatgtaatt gcacaatttc
     1621 tttgcaaatc gtcagcccca gaccagagcc gccccggctg cgcgaagtct gattgcgcgc
     1681 ttgctgaaag cgatcgaata cgtttttgag cattgccggc tcaatgcccg ccccttcgtc
     1741 agtgacgctg aaagtcactg tctgcccgct gttatgggcc cccagagtga cggtacctcc
     1801 actggcggaa aactttatgg cattggaaac cagattggaa attactcgct gcagcatttc
     1861 ctcatcggcg cgcactacgg atttggtggg ctttactttc aagctgacgc ccatctcggc
     1921 agcgaagcta gtggcttctt cggcagcagc ttccaacacc tcccgggcag atacattgct
     1981 cagattcatc tccatttttc cagacttgat tttctcaata tcgagtaagt ctctaattag
     2041 aagcaccatg cgattgcagc ttcgccgggc gccggcaaga tgcctgtgcg atttatcctc
     2101 cttggggccg acctccgact tctcaccata aaatccctga ctgagaagct ctagaaagct
     2161 ctgcaccgtc atgagcggcg tccgcaagtc atgggtgacc attgccacca cttcctgccg
     2221 cagttgctca gcgcgactct gctcagtcac gtcatggaaa atgctgaata gttccccact
     2281 cttcccctct tctgccgccc agcgcgccga ccagagaaca tctcgcactt ctccatcggc
     2341 gcgcctgatg gaaagtttca aggccccctg catctcgccc tggcgagctt tgtcgcgaaa
     2401 ggccagtacg ctctgagcgc agtctttatc aagatagtcc agcaacttgg tagagacaat
     2461 ctcatcatcg ggcacagcaa aaaattgcag actggcggcg ttggccgaag taaagcgcaa
     2521 gtcggaatca agagagcaaa tacaatcgta ggcagactcg agaatggctt tctcacgacc
     2581 agccgcattc ttgagatgac cggccatctg gtgaaaaatt ccatccaccc tggctatctc
     2641 atcatcaccg gctagaggtg gattgagcgg cctgtcactg gccaggcgat aactgttgtc
     2701 gttgataacg ttcagacgac tgacgatgtt tcgggtcagt atcagaccca agaccagcgt
     2761 aagaagcata ttagtgacac caccggcgat gagca
//
LOCUS       DAOQNZ010000435         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19861_length_2788_cov_3.747530, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000435 DAOQNZ010000000
VERSION     DAOQNZ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19861_length_2788_cov_3.747530"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            860..2032
                     /locus_tag="PKW73_08745"
     CDS             860..2032
                     /locus_tag="PKW73_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNA73413.1"
                     /translation="MTTANLLTYCLLLLIFVAANGVFVAFEFALISMRRTRVTELIDD
                     GVLAARAIRRLQNDMDETISGAQVGITFASLVLGWIGEPAVHEILHRAFGLIPAGLIA
                     TIEKFCTSAANFLPEALVSTLQQLPILNGLSFLLSFSLLSLLHVVLGEQVPKGLALRF
                     PERLLLIAAGPFLLYRTMVFPLIFVFSFATKSVLRLMGIKQSANHEPVHSSEELSHLI
                     DGSSVAGEISHAINNVLQKALDLQDLQVSEVMRPLDEVDMVEVNTSLRQLLKVVADCK
                     HSRLPVYKDRRDNIIGVFSTKDLFEIIDDKMSSVALEAALKQLSVDFHLSKHLRPYLR
                     VTDGMQASSLLHMLLIRTMPMAIVINDTEERLCVGISTLEDVLEHLVGEIKDEHDH"
     gene            2119..>2788
                     /locus_tag="PKW73_08750"
     CDS             2119..>2788
                     /locus_tag="PKW73_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA73414.1"
                     /translation="MTETNSKPTLTLPVALPEVQPQWANLALAFVQKAKTCSTRIAVE
                     DDQGSYTYGDLLAKSLAIALILQEQLTDSETVGIILPPSAPAVIANLAISLLGKTPVN
                     VNYGTGNELVNYTLEDAGTDFVISSPSFLQTVKLEPVKTVLDMTELAGRVNQYVLADV
                     AAILAGAEDAKPMPGLAATQASTATLLYTSGSTNRPKGVMLSHESIITNVLAVSERYP
                     TRYTQ"
BASE COUNT          719 a          836 c          635 g          598 t
ORIGIN      
        1 gactatcctg attggtcaag gtaccagatg tcacgacgat cgctcctttg cacattccaa
       61 ccgaagcaaa gcagcagagg gagccccttc tgcttgattg ccagagctta ccactttcct
      121 tggagcggca ttactgcact accggcggta ccgacacttt tgcaacgctt ccttaatcta
      181 atttttctta gtgatggcgg gcaaaaccgc gcccagtata tccatacaaa gacctgacac
      241 ccatacaagg gaaccacaag aagcccccga tttgcaggcc ggttgtaagc caaaagcgat
      301 acccgctacc ggcataaaag atctcaaagc ccagatacaa ggccaggggt tgctacctgc
      361 aagtgtcctt ttaaattgtc gccaaattgt cagccggcag tcaccgattc gacacagacc
      421 ggtcaaaccg gctcccacgc ccacacgaga gcaacagcac ggtcagtgtt aaacgacact
      481 cagaagccga acaggctaaa tacagttacg aaaaggaaga atctcttggc aacaaagacg
      541 gtaaagagct agaacctaat tgcagttaca tcaaccactc tcaaaagagg aaatcattcc
      601 ctaaatcttc aagatcgaac accaacagtg tccagaccat ggagacccca gtcagggagt
      661 ctttctcaga cagacaaact gcaaccctgt caataacaca tacagagttt tcagcactct
      721 tccaggctga aagccggcga accgaatcgg gacacggcca atagaccttg aagtaaagag
      781 aaatcttgca acgctaaaac tgagtccgaa cggtcaccac atcgacgttc gcttccattt
      841 acctacactc catttccata tgaccacagc taacttgctt acatactgcc ttttgttgct
      901 tattttcgtc gctgccaacg gcgttttcgt ggccttcgaa tttgccctca tctcaatgcg
      961 acgcacccgc gtcacggaac taatcgacga cggcgttcta gcggccagag cgatccggcg
     1021 tctacaaaac gacatggatg aaactatatc gggcgcccag gtcggtatca cttttgccag
     1081 cctggtgctg ggctggatcg gtgaacctgc cgtgcatgaa atcctgcacc gggctttcgg
     1141 tttaattccc gctggtctga ttgccaccat tgaaaagttc tgcaccagtg ccgccaactt
     1201 cctgccggaa gcgctggtct ccacactgca gcagttgcca attttaaacg ggctttcctt
     1261 cctgctttca ttcagcttgc tctcgctgct gcacgtagtg ctgggcgaac aagtaccaaa
     1321 agggctggcg ctgcgctttc cggaaagact actgctgatt gcagccggtc cctttctgct
     1381 ctatcgcacc atggtctttc cgctcatctt cgtcttctcc ttcgccacca agtcggtact
     1441 gaggctaatg ggcatcaaac agtcggccaa tcatgaaccg gttcactcca gcgaagagct
     1501 gtcgcacctg atagacggca gcagtgttgc cggagaaatc agtcatgcca tcaacaatgt
     1561 tctgcaaaaa gctctggacc tgcaagactt gcaggtctcc gaggtaatgc gccccctgga
     1621 cgaggtggac atggtggaag taaacacctc cttgagacaa ctcctgaaag ttgtggctga
     1681 ttgcaaacac agcaggttgc cggtttacaa agacaggcgg gacaacatca tcggggtctt
     1741 ttccaccaaa gacctcttcg aaataatcga cgacaaaatg agctctgtgg cgctggaagc
     1801 cgctctcaag cagctcagcg ttgactttca tctttccaaa cacctgcggc cgtatctgcg
     1861 cgtcaccgac ggcatgcagg ccagcagtct attgcacatg ctgctgatca gaacgatgcc
     1921 catggccatt gtcatcaacg acacggaaga aagactctgt gtcggcatca gcaccctcga
     1981 agacgtactg gaacaccttg tcggtgaaat caaggacgag cacgaccatt aaacgctggt
     2041 caccctccat ccggtgcaga caacttctct gcgcccattt atcgccaatt catcgtccat
     2101 tcatcggaga aacgaaacat gaccgaaacc aactctaagc ccactctcac cctgcctgtc
     2161 gccttaccgg aagtgcagcc gcaatgggcg aatctagccc tggccttcgt gcagaaagcc
     2221 aagacctgtt ccactcgcat cgccgtagaa gacgatcagg gttcctacac ctacggtgac
     2281 ttactggcga aatcacttgc catagcccta atcttgcaag agcaactaac ggacagcgaa
     2341 actgtcggca ttatccttcc accttccgcc cccgccgtca ttgccaatct ggccatttca
     2401 ttgctgggca aaacgccggt caacgtcaat tacggcaccg gtaacgaact ggtcaattac
     2461 accctggaag acgccggcac tgacttcgtc atctccagcc cgtccttcct gcaaacagtc
     2521 aagctcgaac ctgttaaaac cgtgctggac atgaccgaac tggcaggacg ggtaaatcaa
     2581 tatgtcctcg ccgatgtagc cgcaatcttg gccggtgccg aagacgccaa gcctatgccc
     2641 ggactggctg cgacgcaagc cagcacagcc accctgctct acacctccgg ttctaccaac
     2701 cggccaaaag gcgttatgtt gagccacgaa agcatcatta ccaatgtgct ggccgtcagc
     2761 gagcgttatc ccactagata cacgcagg
//
LOCUS       DAOQNZ010000436         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19882_length_2787_cov_2.915447, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000436 DAOQNZ010000000
VERSION     DAOQNZ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19882_length_2787_cov_2.915447"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..1475
                     /locus_tag="PKW73_08755"
     CDS             396..1475
                     /locus_tag="PKW73_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73415.1"
                     /translation="MTSSASCQDLTAPPSQHDRMPDPAELLLNAQDAPKITDKLNIFP
                     MEWRVETPRLLDIYESSRDPGWSPGQLPWRTLEVEKMTLDQRYAISYWFALLSVFDAS
                     GPAVFCRAMIHAYETHEEDAIRKCFFSVARDEMNHEEVCGRAITKLTPGGPLGYEPET
                     ELGKLARNNIQWLYHNGARYWNGYKTAVEHYPMAILFSSFLFGEVASSTLFHSMYERT
                     QIPVFKEAFRNIGRDEGRHLSFCLALLKEVLPKLTDEEKSTITKQFRAGFIFLSGILY
                     EPPAEFWQLPPTFMPAQRLLEEKAREAGFGILTLEERRENWRTAVVRLKTIVEPYGVK
                     FPALPEIDVDGEDVAFDCDKIIPIF"
     gene            1560..2153
                     /locus_tag="PKW73_08760"
     CDS             1560..2153
                     /locus_tag="PKW73_08760"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA73416.1"
                     /translation="MVRPRAENYEERRGEILDKAAAMFAERGFDATSMSSIASGLGVS
                     KALVYHYFQSKEELLYQMLHSHCLLLVETAAKAVQSSQKADQQLESLVLELMRLYMSS
                     RDKHVVLMNSLKALSPEQQKEIKALEREIVAHIKTLVQLLRPGLNESEVTSLAMYLMG
                     SINWTYTWFKVSGSISPDKYAEMAAQLFLNGLKGAAL"
     gene            complement(2221..2622)
                     /locus_tag="PKW73_08765"
     CDS             complement(2221..2622)
                     /locus_tag="PKW73_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73417.1"
                     /translation="MLDQRQTATQEKGRTPLHLKQTCAELDTLPLWRKIACALGLSRL
                     SGTKSETTREFLPEPSSELSQNSSPAQARIAESEAVPAVPGVAAESGTPRAKLTYARL
                     ESLDAYLRDKTKREANSLLSSPAPELSSPGA"
BASE COUNT          601 a          734 c          751 g          701 t
ORIGIN      
        1 cgggtctgtc cgtactttcg atgtccggcg gcggcgagca ctctcatcgg atataaccac
       61 tgaatgcaag atcgagcttt tcagttttct tctttcgatc tgccagtctg cgcctcaaaa
      121 tcggaacaga cccagaagca atttccggac agattaaaac tatcccaacc ggttacaaaa
      181 aggttaccag cctgtatata tgctgctaag atattcatta accggccggt tggtcaatga
      241 atgattttcc caatcgagtt ggtctgagag tttttgagga gctagatagg gcagtgacta
      301 caactacttt ggagtgtact caagagatgg ctacaaaaga gaaatcggca aagtcttcgg
      361 catccaagtc atgtgcggag aaagctacga caaaggtgac cagctcagcc tcctgccagg
      421 atttgacggc gccgcccagt cagcacgatc gcatgcctga cccggctgag cttctgctca
      481 acgctcagga tgctcccaaa atcactgata agctcaatat ttttccaatg gaatggcggg
      541 tagaaacacc cagactgttg gacatttacg agagttccag agacccgggt tggtctcccg
      601 gtcagttgcc atggcgcact ctggaagtag aaaaaatgac cctcgaccag cgctatgcca
      661 tttcttactg gtttgctctg ctttcggtct ttgatgcttc cggacctgcc gtcttttgcc
      721 gggcaatgat tcatgcctat gagacccacg aagaagacgc cattcgtaag tgtttcttct
      781 cggtggccag agacgaaatg aaccacgagg aagtttgcgg cagggcaatt acaaagctca
      841 cccccggcgg tccgctcggc tatgagccgg agaccgaact gggcaaattg gcccgtaaca
      901 acattcagtg gctctatcac aacggtgcca gatactggaa cggctataag acagccgttg
      961 agcactatcc catggctatt ctcttcagct ctttcttgtt cggtgaagtg gcttcgtcca
     1021 cccttttcca cagcatgtac gagcgcactc agattcctgt cttcaaggaa gctttccgca
     1081 acatcggtcg tgacgaaggt cgccacctga gtttctgtct ggcgctcttg aaggaagtgt
     1141 tgcccaagct taccgacgaa gaaaaatcca ccatcaccaa gcagttccgt gccggcttta
     1201 ttttcctctc cggtattctg tatgagccgc cggccgaatt ctggcagttg ccgcctacct
     1261 tcatgcctgc acagcgcttg ctggaagaaa aggcccggga ggccggcttc ggcatcctca
     1321 ccctggaaga gcggcgggag aactggagaa ccgccgtggt gcgcctgaag accattgtcg
     1381 aaccttacgg cgtcaagttc ccggccttgc cggagattga tgtggatggt gaagacgtgg
     1441 cctttgactg cgacaagatc attcctatat tctaggagtg cgtatcctat ttcaggaatg
     1501 cgcagttaat tccggttcag ggacacgatg attaatttca aaagcgcaga gtatgcgcca
     1561 tggtaagacc gcgcgccgag aactatgaag agcggcgagg ggaaatcctg gacaaggcgg
     1621 cggctatgtt tgccgagcgc ggtttcgacg ctacgtcgat gtcatccatt gcttccggtt
     1681 taggcgtttc caaggccctt gtctatcact acttccagtc caaggaagag ttgctttatc
     1741 aaatgctgca cagccactgc cttttgctgg tggagacggc tgctaaagct gtgcaatcct
     1801 cccagaaagc cgatcagcaa ctggagtcgc ttgtgctgga gctgatgcgc ctctatatga
     1861 gctcgcggga taagcatgtg gtcttgatga acagtctgaa ggcgctttcg ccggagcaac
     1921 aaaaggaaat caaggctctg gaaagggaga ttgtcgccca catcaaaacc ctggttcagc
     1981 ttctgcgccc gggtttgaat gagtctgagg tgacatctct ggctatgtat ctgatgggct
     2041 cgattaactg gacctatacc tggtttaagg tgagcggttc catctcccct gataagtatg
     2101 cggaaatggc ggcccagctc tttctaaatg gtctgaaagg ggcagcttta tagctatact
     2161 gtcggtaagc tgtgtccgct tccagcccgg gtgcagaccg cagtgccgtt ctagagccag
     2221 ttaggcgccg ggtgaactca gttccggcgc cggggaactc aaaagtgaat tcgcttctct
     2281 ctttgttttg tctctcaagt aggcatctag cgattccaat ctggcatagg tgagcttggc
     2341 tctgggtgtg ccgctttcgg cggcgactcc tgggaccgct gggaccgcct ccgactctgc
     2401 tatccgcgcc tgcgcggggc tgctattttg cgagagctcc gatgatggtt ccggcagaaa
     2461 ctctctggtt gtttccgatt ttgtgccgga tagccttgat agtcccagcg cacaagcaat
     2521 ctttcgccag agcgggagag tgtccagttc tgcgcatgtt tgcttgaggt gcaagggagt
     2581 tcggcctttc tcttgcgtcg ccgtctgtct ttggtcaagc atgtgtttcc ctcctgccgg
     2641 cctgcctttt gacctttggg cgcaacggta tagcccgact ttcctgtggt aagtcgcaag
     2701 caggctgttt tcaccggcgg tttcgatcgt tgagctgttg ccccctttat tcaggttagc
     2761 caggcttgag aagtctgcat atccccc
//
LOCUS       DAOQNZ010000437         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19915_length_2785_cov_2.812821, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000437 DAOQNZ010000000
VERSION     DAOQNZ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19915_length_2785_cov_2.812821"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKW73_08770"
     CDS             <1..275
                     /locus_tag="PKW73_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73418.1"
                     /translation="LLGHMLSQQQRQREEEEAARRARDNSSSSSGTDSTYIAPVYIPD
                     STPSAPDSTPSGGSDGGSSGGSNCGGGSSCGGGSSCGGGGGCGGGS"
     gene            373..885
                     /locus_tag="PKW73_08775"
     CDS             373..885
                     /locus_tag="PKW73_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73419.1"
                     /translation="MLDFLLRRKRDGQAGRVPAPPPTFPDQNAYERMWEAIQNLPIDD
                     PKAAKPLSFRLAREQGWSREFTLRVMEEYRRFLFLMAVSDHSVTPSITVDEAWHLHLI
                     YSKSYWEDLCLYTVGKPMHHNPSGGSADEESGFKDDYRETLILYRRFFGEPPADIWGP
                     ENGKNVKSKG"
     gene            1039..1566
                     /locus_tag="PKW73_08780"
     CDS             1039..1566
                     /locus_tag="PKW73_08780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNA73420.1"
                     /translation="MENADKQEEDQQQQRQQLQQEVKLRQVDWDRDFPQLLAIERQSF
                     RSPWDSRKFAVYSSLPKNRALVAELTGQEGIVGYMLYELQATSSNYLGSSTHIAHIAV
                     DPRHRRLGIGRLMIGAVTRSDAVTLYARLSNTDAQRLYVSLGFYIHNIVTAHYSDGED
                     AVLMRWGQEGPYAMQ"
     gene            2442..>2785
                     /locus_tag="PKW73_08785"
     CDS             2442..>2785
                     /locus_tag="PKW73_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73421.1"
                     /translation="MKTTFNPSNQMSALSGILMQNQSDHFHFNRNWNQLFPQFAAHLL
                     EETGNFCGTTEQRTLILELCQICHSSTAASKDKMHLRHLGSSLEGEITAILTTGRYEE
                     SSLKALLSAWALT"
BASE COUNT          686 a          896 c          680 g          523 t
ORIGIN      
        1 ccctgctcgg ccacatgctc tcgcaacagc agcgtcagcg ggaagaggaa gaggcggcgc
       61 gccgggctcg cgacaacagt agcagcagca gcggtaccga ctccacctac atagcgccgg
      121 tctacatccc cgactccacg ccctcggccc ccgactccac accctccggc ggcagcgacg
      181 gcggttccag cggtggctcc aactgcggcg gcggctccag ctgcggcggc ggttccagct
      241 gcggcggtgg cggcggctgc ggcggcggtt cctgagagcg gtcttcacgc tcaagaccac
      301 tccgtctaat aacatgcgca gctgttgccg ggcaacagct gcgctttcct aaaacccaac
      361 ggaggagacg acatgctcga ttttctctta cgccgcaagc gggacggaca ggcaggccgc
      421 gtgccggctc cgccacctac cttcccggac cagaacgcct atgaacgcat gtgggaagcc
      481 atccagaatc ttcccatcga cgaccccaag gccgccaagc cgctttcttt ccgcttggct
      541 cgcgaacagg gatggtcaag ggaattcacc ctgcgcgtca tggaagaata ccggcgcttc
      601 ctcttcctga tggctgtgtc cgatcactcg gttacaccct ccatcacggt tgatgaagcc
      661 tggcatctgc acctgattta cagcaagtcc tactgggagg acctctgcct gtacacggta
      721 ggcaagccca tgcaccacaa tccatccggc ggcagcgccg acgaggagag tggcttcaag
      781 gacgactacc gcgaaacact catcctctac cgccgcttct tcggagaacc acccgcagac
      841 atctgggggc ccgagaacgg caagaacgtc aagtccaaag gctgatgcgg tctgggtatc
      901 cagaccgcgc cgctcgcgat atggcaggcg agaaatcgcc tgttcatcaa aggcgcggca
      961 actgggacag gcaccagcaa gccggtcaaa ggcgacagcg cctttgacca caactcaact
     1021 ctatatcgcg agcccctcat ggaaaatgcc gataagcaag aagaagacca acaacagcaa
     1081 cgccaacaac tgcaacagga agtgaaactt cgccaggtcg attgggacag agacttccct
     1141 caactcctgg ccatcgaaag gcaaagcttc aggagcccct gggattcccg caaattcgcc
     1201 gtctactcca gcctgcccaa gaaccgcgcc ctggtggccg aactgaccgg acaggaaggc
     1261 atcgtcggct atatgctcta cgaattgcag gccaccagtt ccaactacct gggcagcagc
     1321 actcacatcg cgcacatcgc cgtggatccc cgccaccgtc gcctcggcat cggccgcctc
     1381 atgatcggcg ccgtcaccag aagcgacgcg gttacgctct acgcccgact ctccaacaca
     1441 gatgctcagc gcctctatgt cagcctcggc ttctatatcc acaacatcgt cacagctcat
     1501 tacagcgacg gtgaagatgc cgtgttgatg cgctggggtc aggaaggccc atatgcgatg
     1561 cagtaacgcc catccatttc tgcggtctcc gcctttgacc cggcggtcga gagcggggac
     1621 agtaccaacc aaaaacaaca ggaggcctcg gtgccatcga aacgctctgc aagcgagtcg
     1681 acagcgccga ggccgtccgc cacccgtctg ctctctggaa aggcaatgct ttctatgacg
     1741 agcagacagc ttcgggcacg gaccttttct tttttcaatt gcttactaca ccttttagta
     1801 tcttccaccg ttttcatctt ctacaagcgg cgccaaccac agcaacactt gctctgggac
     1861 ccgttgccag cccttcccgc ttgtggcgaa acagtgtcgc tcttcagccc ggtttgtcga
     1921 ctttcgctga gacactatgg gctgcgccaa tccctcaagc ccgggtaact ctgcggaaaa
     1981 tacccaggca gcccacttcc ggctgcaatt tactccccta aaatgtttcc gactcacccc
     2041 cgaaaagctt gaggggtagc gccttctcaa gcgcagcagg caatctacct ggacattcta
     2101 gagaccggat ctccagttca agaaatactt tgaacagaca aaccaaaaac ctatcatgaa
     2161 ctcaccctaa cctttgtttt attactggac aactcaaaac ctttagaaac cccaaaagcc
     2221 aaccgctctt ctattcatga aaagtaaaac cgcaccctgg tgccggtcag gctaaataga
     2281 gtttcaacaa aagacgaaaa cctggtcccc aaaacaacgc gctagagaac caatgcaaac
     2341 ctggtcactc aagagagtca ccatgcaaac tatgcgcttc gtgcaaatga ggtgctgtat
     2401 ctaaaatcga aaatcaatat ttgaaaagca ggtatagtgt catgaaaaca acattcaacc
     2461 caagcaatca gatgtccgcc ctgagcggca tactgatgca aaaccagagc gaccatttcc
     2521 atttcaatcg aaactggaat cagctcttcc ctcaattcgc cgcccactta ctggaggaaa
     2581 ccggcaactt ctgcggcacc actgagcaaa gaaccttgat cttggaactt tgccaaatct
     2641 gccattccag cactgccgcc agtaaagaca aaatgcattt gcgtcatttg ggaagcagcc
     2701 tggaaggcga gataactgcc atcctgacca ccggacgcta cgaagaatcc agcctgaaag
     2761 ccttgctttc tgcctgggcc ctgac
//
LOCUS       DAOQNZ010000438         2783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19937_length_2783_cov_3.673754, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000438 DAOQNZ010000000
VERSION     DAOQNZ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19937_length_2783_cov_3.673754"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PKW73_08790"
     CDS             complement(<1..871)
                     /locus_tag="PKW73_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HNA73422.1"
                     /translation="MSNATSQEHTIRPEGGQEKSADGLFSPLKIKSITLKNRIAISPM
                     CQYHSVDGFAEDWHLVHLGSRAVGGAGLVVMEASGVEARGRISPDDLGIYKDEHIEKL
                     KQITTFISSFGAVAGIQIAHAGRKASTRNPWKGGNRHEKEYLTSENGGWEIVGPSPLP
                     FSESSTVPKELTIEEIHSIQDSFAAAAKRAYAAGFKWLEVHAAHGYLLHSFYSPLSNK
                     RSDKYGGSLENRMRNTLETVEKVRTAWPEELPLTVRISASDWAEGGWTPEDSVILARA
                     LKERGVDLIDCSSG"
     gene            1115..2515
                     /locus_tag="PKW73_08795"
     CDS             1115..2515
                     /locus_tag="PKW73_08795"
                     /inference="COORDINATES: protein motif:HMM:NF015012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HNA73423.1"
                     /translation="MAFNRFPSPARKVLSPLRVFGLGALLLGYCAWSGAGYCQSSSLT
                     SELVSDSLPPAPKVESSQASALSSKKAKRVREKKTKVKDTARVQEKVQDVPVLPAGEN
                     PSDLVPSQHFLGKPEVVAVFNDAMPTGVAVSFSGRRFVNFPRWEDNVPFTVGELSHGK
                     VQPYPDAAFNKLDLKAPENHLISVQSVVVDARDRLWLLDTGSVKFGTVIPGGAKLVCV
                     DLSTNQVIKTIRFPADVALPTSYLNDVRFDLRRGKAGMAFITDSSSSGHNALVVVDLA
                     SGESFRRLVGHASTRAVPGFLPIVEGAPLMFVHEDGERTSMKVGADGIAVGADGKHLF
                     YCPLSSRKLYSVNLDALADRNVSDDDVAKTVCEVTEKGASDGLEMDSAGRIYATDYEH
                     NAVHRISLEGKHETLVCDPRVLWPDTLCLAQDGYLYFTANQLHRQPLFHGGRDLREKP
                     YVLFRVKVDAEPVLLR"
     gene            complement(2517..>2783)
                     /locus_tag="PKW73_08800"
     CDS             complement(2517..>2783)
                     /locus_tag="PKW73_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73424.1"
                     /translation="EKQVERRAAKATRAKVDDGPRPGKVRHSSFSRGLLKTLNFVGFP
                     IGDENLTLEKQSEQVVPPEVQEALGSQNNQAPEGATAPIQSSSP"
BASE COUNT          534 a          742 c          790 g          717 t
ORIGIN      
        1 agccgctgct gcaatctatc agatctactc cgcgttcttt caatgccctg gccagtatca
       61 ccgagtcttc cggcgtccaa ccaccctcgg cccagtcaga agctgaaatg cgcacagtca
      121 aaggtagttc ttcgggccag gcggtccgca ctttttccac tgtttccagg gtgttgcgca
      181 tgcgattttc caggctgccg ccgtatttat cgctgcgctt attggagagc ggcgaataga
      241 aactgtgcag caaatagcca tgggcggcgt gcacctccag ccatttgaaa ccggctgcat
      301 aagcgcgttt tgccgcggcg gcaaagctgt cttgaatgga gtggatttct tcgatggtca
      361 gttcttttgg taccgttgat gactccgaga atggaagggg cgatggcccg acaatttccc
      421 agccgccatt ttcactggtc aaatattctt tctcgtggcg attgcctccc ttccagggat
      481 tgcgagtgga ggctttgcgc cccgcatggg caatctgaat tccggccacg gcgccgaagg
      541 agctaataaa ggtagtgatc tgcttgagct tttcaatatg ctcatcttta tagatgccga
      601 gatcatccgg tgagatgcgc ccgcgggctt ccacaccact tgcttccatt accaccagac
      661 cggcgccgcc tacggctctg gagcccagat gcaccaggtg ccagtcctcg gcgaagccat
      721 ccactgagtg gtactggcac attggtgaga tggcaatgcg gttttttaag gtaatcgatt
      781 tgattttcag ggggctgaaa agaccatccg cagacttttc ttgtccgccc tcaggtctga
      841 tggtgtgctc ctggctggtg gcgttcgaca tgcctgttcc tcatttaaat tggcggtatt
      901 ttaagggcgg tgccctgcaa ggtcgctgtt cggaatatcg ttggcgatta acttcttcgc
      961 aatagaccgg tcggctagtt ccgtcaaccc ttgagaaact tggttgaccg agcttaattt
     1021 accgtggcct tttccggtcg cagttctttt gtgtcagtat tttagaacga agattcttct
     1081 atagtatgaa tacttgattt gtcttgagga aattatggct tttaaccgat tccccagtcc
     1141 tgccaggaaa gtgctttccc ccctccgggt ctttggacta ggcgccctgc ttctaggcta
     1201 ttgcgcctgg agtggagctg gctactgcca gagttccagc ctgacgtcgg agttggtaag
     1261 cgactccctg ccaccggctc caaaggtcga gtcaagccag gcttccgcac tttcttccaa
     1321 gaaggcaaag cgggtgcggg agaaaaagac gaaagtgaaa gatactgcca gggtgcagga
     1381 aaaagtgcag gatgtaccgg tcttgccggc cggagagaat cctagcgatc tggttccttc
     1441 tcaacacttt ctgggtaagc ctgaagtagt tgctgttttc aatgatgcca tgcccaccgg
     1501 tgtagccgtt tcgttttccg gtcgccgctt tgtcaatttc ccccgctggg aagacaacgt
     1561 tccctttacg gtcggtgaac tttcccatgg caaggtgcag ccctaccccg atgcggcttt
     1621 caacaagctc gatctgaagg cgccggagaa tcacttgatc tcggtgcaga gtgtggtggt
     1681 ggatgcccgg gaccgactct ggcttctgga taccggctcc gtcaaatttg gcacggtcat
     1741 accgggcggt gccaagctgg tttgtgtgga cctgtccacc aatcaggtca tcaaaacgat
     1801 tcgctttcct gccgatgtgg ctttgcccac cagttatttg aacgatgtgc gctttgactt
     1861 gcgccggggc aaggccggta tggccttcat tactgattcc agctccagcg ggcataatgc
     1921 gctggtggtt gtggatctgg ccagcggcga gagttttcgc cgcctggtag ggcatgcttc
     1981 caccagggcc gtgcccggct ttttgccgat tgtagagggc gcgcctctga tgtttgtgca
     2041 tgaagacggt gagcgcacca gtatgaaggt cggcgctgat ggtatcgccg ttggtgcgga
     2101 cggtaaacat cttttctatt gtcctttgag cagccgtaag ctttacagcg tcaatttaga
     2161 tgcactggcc gaccgcaacg tcagtgatga tgatgtggcc aagacggttt gcgaagtaac
     2221 cgaaaagggc gcctcggacg gactggagat ggactcggcc ggccgcatct atgccaccga
     2281 ttacgagcat aatgccgtgc atcgaatttc tctggaaggc aagcatgaga ccctggtatg
     2341 cgacccgcgc gtgctctggc ccgataccct ctgcctggcg caagacggct atctttattt
     2401 tacggccaac caattgcacc ggcagccgct cttccacggt ggtcgcgatt tgagagaaaa
     2461 gccttatgtg ctcttccgtg tgaaagtgga cgccgagccg gtgctcttgc gctaactcag
     2521 gggctgcttg actgaattgg ggcggtggcg ccctcgggcg cctggttgtt ttgagagcct
     2581 aatgcttctt gcacttccgg cggaactacc tgctcggatt gtttttccag agtcaggttc
     2641 tcatctccga tgggaaagcc cacaaagttc agggttttga gaagccctcg ggagaagctg
     2701 gaatgccgga ccttgccggg gcgagggccg tcatcgactt ttgcgcgggt agcttttgcg
     2761 gcccttctct ctacttgctt ttc
//
LOCUS       DAOQNZ010000439         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19955_length_2782_cov_3.332967, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000439 DAOQNZ010000000
VERSION     DAOQNZ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19955_length_2782_cov_3.332967"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PKW73_08805"
     CDS             <1..755
                     /locus_tag="PKW73_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNA73425.1"
                     /translation="LVVSPLGLDLDFWKSKAGAGVWSVSSPSVELVKSRPFVLSVDRA
                     DYTKGVLERIHAIEKFFEAFPERIEQVSFLQVCQPTRQGLAAFDNYYRQCREVTDYVN
                     GRFRQGDWQPIVWVDEPLPQSALIELYRSAGGMLVNPVRDGLNLTAKEFVGSSAANPG
                     ALLLSPGAGVFAELGGYCSPVDPSSPTQMASAIEGALTMSLPERQERISRMKDILRAH
                     TLEDWWTTFAVSPSDALTDMTCSEDVREAIGF"
     gene            848..>2782
                     /locus_tag="PKW73_08810"
     CDS             848..>2782
                     /locus_tag="PKW73_08810"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HNA73426.1"
                     /translation="MARTKKVSADTDQELPYEVDSRPLCQGMRFVLTDAAHPRKVLKH
                     GSHFLVLDAAGMIPACNTLGYGYYRDDTRYISQWEMALDDAPISLLSSRIEDGFSATL
                     FYTNPQTANLQQQRITLLREVVLGDALYERITLENFHSEEVEFDLTISLQSDFADMFE
                     VRGLNLSQRGERMVPLSGKDGRCLSLAYKGLDGLLQETVIDIDASGAQRASLQDGRFT
                     IRFRLPVRQSRSISLKLLTRLNGKTAPVSHDEVDYASALKLATNRYLNWCKSVVDIQT
                     CQELFDVSLNRSLKDIYILRQPTPKGIGLAAGIPWYCAVFGRDSAITALQLLPFAPEL
                     ARETIEVLAAYQGQKDDLYKAEHPGRIMHELRLGELARRAEIPHSPYYGTVDATQLWL
                     ILYGQYVSWSGDIEFARRLWGSVYLALDWLDRASGSGYITYQRESPHGLENQGWKDSG
                     DSVMHRDGRLANPPIALCEAQAYLYQAWQEVASVAVLLGKSEIADSLVVKARSLKDRF
                     QRDFWMSDENFLALALDRDGCQVGSISSNPGHCLASGILDEDKAHLVADRLMSLELHT
                     SWGIRTLSSGASSFNPISYHNGSIWPHDNSMITEGLRKVGRVEDVHSIMRELFEVMLL
                     QPDFRLPELFCGFDKIDAAAP"
BASE COUNT          587 a          679 c          795 g          721 t
ORIGIN      
        1 aattggtggt atcgccactg gggcttgatc tcgatttctg gaagtctaaa gccggtgccg
       61 gcgtctggtc ggtatcttcc cccagcgtag aactggtgaa gtcgcgcccc tttgttctgt
      121 ctgtggatag agccgactac accaagggtg ttctggagcg cattcatgct attgagaagt
      181 tcttcgaggc ttttcctgaa cgaattgagc aggtttcatt cttgcaagtg tgtcaaccaa
      241 ccaggcaagg cctggctgct ttcgacaatt attaccggca gtgtcgcgaa gtcaccgatt
      301 atgtcaatgg ccgcttccgg cagggtgact ggcagcccat agtctgggtg gacgagcctt
      361 tgcctcagtc ggcgctgatt gagctctacc gcagtgccgg cggtatgttg gtcaatcccg
      421 tgcgggatgg gctgaacctc acggccaagg agtttgtagg tagttccgct gccaatccgg
      481 gtgcactgct cctttcaccg ggcgccggcg tgtttgcaga attgggtggc tattgtagcc
      541 ctgtcgatcc ctcttcgccc acgcaaatgg cttccgccat agagggtgcc ttgaccatgt
      601 ctttgcctga gcggcaggag cgaatctcgc gcatgaagga tatactgaga gcacatacat
      661 tggaagactg gtggacgact tttgcagttt cgccaagcga tgcgcttact gatatgactt
      721 gttcagagga tgtgcgtgag gcgattggat tctaagttgc agcaagggtt tatcccactg
      781 tgcaaaacat acgattcggc tgggaactct cttccgttgt gcggcgagtt tctgggagag
      841 gtatgaaatg gccaggacta agaaagttag tgccgacacc gatcaagaac tgccgtatga
      901 ggtagacagt cgtccgctct gccagggcat gcgctttgtc ttgaccgatg ccgctcatcc
      961 gcgcaaggta ctcaagcacg gcagtcattt tcttgtcctt gatgccgccg gaatgattcc
     1021 agcttgcaac acgctcggct acggctacta ccgtgatgac actcgctata tctctcagtg
     1081 ggagatggct cttgatgacg cgccgatttc tttgctgtcc agtcgtatag aagacggctt
     1141 ttctgccact ttgttttata ccaatccaca gaccgccaat ctgcaacaac agcgcatcac
     1201 tcttttgaga gaggtggtgt tgggagatgc gctctatgaa cgaattacgc ttgagaactt
     1261 tcatagtgaa gaagtggaat ttgatctgac aattagcctg cagtctgatt tcgcagacat
     1321 gttcgaggtt cgaggcctga atctctctca aagaggtgag cgcatggtgc cgctgtccgg
     1381 caaggatggc cgttgccttt cgcttgcgta taaaggtttg gatggtttgt tgcaagaaac
     1441 cgtgattgat attgatgcct ccggagcgca gcgggcctct ctgcaagacg gcagatttac
     1501 gattcgcttc aggttgccgg tcaggcagag ccgtagcatt agtttgaagc tgctgacccg
     1561 gctcaatggc aagactgctc ccgtaagtca tgatgaagtg gattatgcca gcgctctcaa
     1621 gctggcaacc aatagatatc tgaattggtg caagtcggtg gtggatatcc agacttgcca
     1681 ggagcttttt gacgtatctc tcaaccgcag tttgaaagac atatacattt tgcgtcagcc
     1741 gactccaaaa gggatcgggc tggccgcggg gattccctgg tattgcgccg tctttggtcg
     1801 cgattcggcc attacggctc tgcaactgct tccctttgcc cctgagcttg ctcgtgaaac
     1861 tatcgaggtt ctggctgcct atcaagggca gaaagacgat ttgtataagg ccgaacaccc
     1921 cggtcggatc atgcatgagc tgagattggg cgaacttgca aggcgggctg agattcctca
     1981 cagcccctac tacggcacag tggatgccac gcaattgtgg ctcattctct acgggcagta
     2041 cgtatcctgg tctggggata ttgagtttgc tcggcggctg tgggggtctg tgtatttagc
     2101 gctggattgg ttggatagag caagcggctc aggttatata acttaccagc gggagagccc
     2161 acatggtctg gaaaaccagg gctggaaaga ttcgggagat tcagtcatgc accgggacgg
     2221 gcgtttggca aacccgccca tcgctctatg cgaggcgcaa gcctatctct atcaggcctg
     2281 gcaggaagta gcaagcgtgg cggtattgct gggcaaaagc gagatagccg attctctcgt
     2341 cgtcaaagcc aggtctttga aggatagatt tcaaagagac ttttggatga gcgatgaaaa
     2401 ttttctagct cttgctctgg atcgagacgg ctgccaggtt ggttctataa gctcgaaccc
     2461 cgggcattgt cttgcctccg gcatactcga tgaagacaag gcccatcttg tggctgatcg
     2521 cctcatgagt ctggagttgc atacaagttg gggtattcgc acgctttcct ccggggccag
     2581 ttcctttaat ccgatcagct atcacaacgg ctctatctgg cctcacgaca attctatgat
     2641 cactgaaggc ttgcgtaagg tgggtcgggt tgaggatgtt cactcgatta tgagggagct
     2701 atttgaagtg atgctgctgc agcctgattt tcgactgccg gaattattct gtggttttga
     2761 caagattgac gccgccgcac cc
//
LOCUS       DAOQNZ010000440         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19988_length_2779_cov_4.760646, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000440 DAOQNZ010000000
VERSION     DAOQNZ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19988_length_2779_cov_4.760646"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1085
                     /gene="moaA"
                     /locus_tag="PKW73_08815"
     CDS             93..1085
                     /gene="moaA"
                     /locus_tag="PKW73_08815"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNA73427.1"
                     /translation="MSEPVNLLVDSFGRKHTYLRISVTDRCNLRCQYCMPAAGLKWKE
                     KAELLTFEEIEKLTRLFTAMGICKVRLTGGEPLVRKNLLRLVEMLCAVPGLKTLAMTT
                     NATLLAPQAEALKHAGLKHLNISLDSFDRSRFQEITRMDALPEVMAGIESAIAAGFPV
                     LKLNMVPIAGFNDDEILDFAHFAQKHKINVRFIEFMPFKDNQWQADKVVTFAEMKARL
                     EKHFHLEAMDQPQGEVARDYALRGGGTVSFITSMSESFCRSCNRLRLTSDGSMKSCLF
                     FPPEINLRDALRQNAPEEALRGMILACLAGKPEAHPPAEEIAAAENRSMIEIGG"
     gene            1096..2064
                     /gene="moaCB"
                     /locus_tag="PKW73_08820"
     CDS             1096..2064
                     /gene="moaCB"
                     /locus_tag="PKW73_08820"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HNA73428.1"
                     /translation="MRDVSQKVSTLRTAMAKAILRVSPGTIEQIKKGTLPKGNPLEVA
                     RVAAVQAAKNTSQIIPYCHPLPIDYVGVEFHLDEDFIEIETTVKAIYKTGVEMEALTA
                     ASVAALTIYDMAKMVDDLMQIESVTLMSKTGGKSDYQADSAGATRKAAVLTLSDSISR
                     NKGEDVSGKILLAALEEQGFQVIEYAVLPDDESEIVPAVRRICDELKADLLLTTGGTG
                     LSPRDNTPEALKRLIERELPGISEALRAYGQERNSFSMLSRGLAGLRDKTIIVALPGS
                     PSAAQDGVKVLFPVVNHAFKMLAGQGHKHNHSHDKGRGNESVQSKH"
     gene            2039..>2779
                     /locus_tag="PKW73_08825"
     CDS             2039..>2779
                     /locus_tag="PKW73_08825"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73429.1"
                     /translation="MNPYKANTEVAVLTYEEALQAILRESARIERQEELVDLEESAFR
                     VLAKPLTAPFDLPRFNNSAVDGFGVLVEDLNGDAEAVLTLKSTIAAGDGNGHQTLKSG
                     ECIKILTGAPVPDWVEAVVMKEFCQEEEGTIKVKARVRIGENIRMTGEELEEGATVLE
                     AGTLLSPPALGLAATAGAAQVTCFSQPRICIVSSGDELKEPGQALSASQIYNSNSYAV
                     EAALKGLYWRSENIKRLHTADEPQAVKDT"
BASE COUNT          721 a          799 c          748 g          511 t
ORIGIN      
        1 gccatcaagc acagattgcc tatttggaag aaggaagaat acctggacgg cagcaccagc
       61 tgggtcaact gccagtgccg ggaggacagc ccatgagcga gccggtaaac ctactggtag
      121 acagcttcgg tcgcaagcat acttacctgc gcatctccgt caccgaccgt tgcaacctgc
      181 gctgccagta ctgcatgccg gccgccggac tgaagtggaa agaaaaagcg gaattactca
      241 ccttcgagga aatcgaaaag ctgaccagac ttttcacagc catgggaatt tgcaaagttc
      301 gtctcaccgg cggcgaacct ctggtgcgaa aaaatctttt acggctggtg gagatgctct
      361 gcgccgtacc gggtttaaag actctggcca tgaccaccaa tgccaccctt ttggcgcccc
      421 aggcggaagc cctcaagcat gccggtctca agcacctcaa tataagcctg gacagtttcg
      481 accgcagccg ctttcaggag atcacaagaa tggatgccct gccggaagtg atggccggca
      541 tcgaaagcgc cattgccgcc ggttttcccg tgctcaaatt aaatatggtg ccgatagcag
      601 gcttcaatga cgacgaaatt ctcgactttg cccacttcgc tcaaaagcac aaaatcaatg
      661 tgcgcttcat agaattcatg cccttcaagg acaaccagtg gcaggccgac aaagtcgtca
      721 cctttgcaga aatgaaagcc cgactggaaa agcacttcca ccttgaagca atggaccagc
      781 cacaagggga agtagccaga gattacgctc ttagaggcgg cggcacggtc agtttcatta
      841 cctccatgtc cgaaagtttt tgccggagct gtaatcgcct ccgtctgacc agcgacggct
      901 caatgaagag ttgtcttttc ttcccgcctg aaataaatct tcgcgatgcc ctgcgccaaa
      961 atgcaccgga ggaagcgctt cggggtatga tcttggcttg ccttgcaggc aagccggaag
     1021 ctcatccgcc cgcagaagaa attgcggcgg ctgagaatcg ctcgatgatt gaaattggag
     1081 gctaaagcca gaaccatgcg cgacgtttca caaaaagtga gcaccctgag aacggcaatg
     1141 gctaaggcta ttctccgggt cagtcccggt acgatcgagc agattaagaa aggcacgctg
     1201 cccaagggta atccactgga agtggcaagg gtagcggcag tgcaagcggc caagaatacc
     1261 agccagatca ttccctactg ccatcctctg cccatcgact atgtaggcgt cgagtttcac
     1321 ctggacgaag acttcataga aattgagacc accgtcaagg ccatatacaa gaccggcgtg
     1381 gaaatggaag ccctgacagc ggcttcggta gcggctctga ccatttacga tatggccaaa
     1441 atggtggacg atttgatgca aatcgaaagt gtcaccctca tgtccaagac cggcggcaaa
     1501 agcgactacc aggccgattc cgccggcgcc acccgtaaag cagcggtact gactctttcc
     1561 gatagcatct cccgcaacaa aggggaagac gtgagcggca aaattcttct agcggccctg
     1621 gaagaacagg gctttcaagt aatcgaatat gccgtcttgc ccgacgacga atcggagata
     1681 gtaccagccg tcagacgcat ttgcgacgaa ctgaaagccg acctgcttct caccaccggc
     1741 ggtaccggct tgagcccccg agacaataca ccggaagctc tcaagcgcct cattgaaaga
     1801 gagcttccag gcatctccga agccctgaga gcctacggac aggagcgcaa ttctttcagt
     1861 atgctcagcc gaggtctggc cggtctcaga gacaaaacca ttatcgtcgc cctgccaggc
     1921 tccccatcgg cagctcaaga cggcgtgaaa gtgctctttc ccgtggtcaa ccatgccttc
     1981 aaaatgctgg ccggtcaggg gcacaaacac aatcacagtc acgacaaagg tcgtggcaat
     2041 gaatccgtac aaagcaaaca ctgaggtagc cgtgctcact tacgaggaag cgctgcaagc
     2101 aatccttaga gaaagtgccc ggattgagcg gcaggaagag ctggtcgacc tggaagagag
     2161 cgcctttcgg gtactggcca aaccgctgac agcgcccttc gacctgcctc gcttcaacaa
     2221 ttcggctgtg gacggcttcg gtgtattggt ggaagacctg aacggcgacg ctgaagcggt
     2281 cctcaccctc aaatcgacta ttgctgccgg tgatggcaac ggtcaccaga cactgaagag
     2341 cggcgagtgc ataaagattc tcaccggcgc tcccgtaccc gactgggtag aggccgtggt
     2401 catgaaagag ttctgccagg aagaagaagg cacgatcaaa gtcaaagccc gggtgcgcat
     2461 cggcgaaaac atccgcatga ccggcgaaga actggaagaa ggagccacgg tgctggaagc
     2521 cggcaccctc ttaagcccgc cggccctggg actggcggcc acggccggcg cagcgcaagt
     2581 gacctgcttc agccagcccc gcatctgcat agtttcaagc ggcgacgaat tgaaagaacc
     2641 aggtcaggcc ctcagtgcct cccaaattta caactccaat tcctatgccg tggaagccgc
     2701 cctcaagggc ctctactggc gcagcgaaaa cataaagcgc ctgcacactg ccgatgagcc
     2761 ccaggcagtg aaagacacc
//
LOCUS       DAOQNZ010000441         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_19992_length_2779_cov_3.740088, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000441 DAOQNZ010000000
VERSION     DAOQNZ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19992_length_2779_cov_3.740088"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKW73_08830"
     CDS             complement(<1..547)
                     /locus_tag="PKW73_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA73430.1"
                     /translation="MAQARQIDPERIYDAVIIGAGISGLTAAGHLVRNGFQPLILEAE
                     KEPGGRVRTIIQDGYHLDLGFQVFLTAYPEAMRQLDYARLDLCPFQSGALVHYNQAFT
                     RIADPFREPTALFSTLISDIAPMADKLKIAYLRQKLMNMPVDEIFRQPERTIKKALSD
                     YGFSNRIIDRFFRPFFGGITLD"
     gene            complement(525..878)
                     /locus_tag="PKW73_08835"
     CDS             complement(525..878)
                     /locus_tag="PKW73_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA73431.1"
                     /translation="MNRQTELDSIDHIAIQVKDLAKTVDFYKESFNCKVIYQDDTWAF
                     LRFGNIKLAFVVPGQHPPHLAFISEEAESFGELKLHRDGTRSIYIQDPSGNTLEIMAP
                     YDDTEAKDSGTSQAN"
     gene            complement(937..1797)
                     /locus_tag="PKW73_08840"
     CDS             complement(937..1797)
                     /locus_tag="PKW73_08840"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNA73432.1"
                     /translation="MSEKETKVKEVGSYGGIATGGHTPLSYNSYLMVDELKKLQVCQS
                     EPAHHDEPLFIIIHQTYELWFKLIIHELDLVVELMREDKVRRATHFMRRVVSIMRVLV
                     QQIHILETMAPRDFLGFRHKLNPASGFQSSQFREIEFMAGLKEERFLQHFKQDEVAFE
                     ALTRRFNEQSLEDIFYDLLRRKGFKLTMPKAGADETLVRELEQERIKELIALYDEDSK
                     WIDLHDLAEKLIDLDELISLWRTHHVTVVERIIGFKRGTGGSEGVGYLRSTLYKRCFP
                     DLWHLRTALE"
     gene            complement(1836..>2779)
                     /locus_tag="PKW73_08845"
     CDS             complement(1836..>2779)
                     /locus_tag="PKW73_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA73433.1"
                     /translation="QVEGPGRDETHWQLFIDVAQVLNAPWYFIMGEQDISGPIPVDKM
                     RTYGPDLKGRGLNSPDTYWSLDPVSGLHLIGLDTARANSNTGEMRSQQLEWLKEDLKK
                     NSGKFTVVASHHPLLPPPPYDSGPPWEEYIVSQGAQAREILGSSRDVRLVLSGHVGAN
                     KIQKERDIWYVSCPSLAVYPCQFKIFRLSAGGILVETYQITYEALVKKAKKELASSRL
                     AFQYSSRKPLTYLKIVEGEELDRQSYLPLAPGAVPLKPAKKAKKPKSNKKEKPPKAKP
                     EPQEKIMKNSTKPTEPAATPENQVDLKSKMPKQSKDN"
BASE COUNT          596 a          681 c          717 g          785 t
ORIGIN      
        1 catcaagggt gataccaccg aagaaggggc ggaagaaacg atcaatgatg cgattggaga
       61 agccgtaatc ggaaagagct ttcttgattg tgcgctccgg ctgcctgaat atctcgtcta
      121 ccggcatgtt cattaatttt tgccttaaat aggcaatttt caacttgtcc gccatggggg
      181 caatgtcgga aatcagagtg gaaaacaggg ccgtaggctc ccggaaggga tcggcgatgc
      241 gggtgaaggc ttggttgtag tgaacaagcg cgccgctttg aaaggggcaa aggtccaggc
      301 gagcatagtc gagctgacgc atggcttcag gataggctgt taggaagacc tggaaaccca
      361 gatcaaggtg gtagccgtct tgaattatgg ttctcactct accgcccggt tctttctcgg
      421 cttccagtat gagaggctgg aagccgtttc tgaccagatg accggcggcc gtcagaccgg
      481 atatgccggc tccgattatg actgcgtcgt agattctttc aggatcaatt tgcctggctt
      541 gtgccactgt cttttgcctc agtatcatcg tagggagcca tgatctccag agtgttgcca
      601 cttggatcct gtatatagat agatcttgtg ccgtccctgt gcaatttcaa ctctccaaaa
      661 ctttcagctt cttctgaaat gaaagccaga tgcggcggat gctgaccagg aaccacaaag
      721 gccaacttga tattgccgaa acgcaggaat gcccaggtat cgtcctggta aattaccttg
      781 caattgaaag actccttata gaagtccacg gtctttgcca gatccttaac ctgaatggca
      841 atgtggtcaa tggaatccag ttctgtttgc ctgttcatgt cttcttcttc tctttgacct
      901 gactcctgct ctttattcct gtttactttt tcctgtttat tcaagggcgg tgcgcaagtg
      961 ccagaggtct gggaagcaac gcttgtagag tgtgctgcgc agataaccga cgccctcgga
     1021 accccctgtg ccgcgcttaa agccgatgat acgctctacc acggtgacat ggtgtgtgcg
     1081 ccagagagag ataagctcat ccagatcgat aagcttttcg gccaggtcat gcaagtctat
     1141 ccacttgctg tcttcatcat agagagcgat tagttccttg attctctctt gttccaattc
     1201 tctcaccaag gtttcgtcgg ctccagcctt gggcatggtc agcttgaaac cttttcttcg
     1261 caaaaggtcg taaaaaatat cttccaggct ttgctcgttg aatcttctag tgagtgcttc
     1321 gaaagctact tcgtcttgct taaagtgctg aagaaagcgc tcttccttca aaccggccat
     1381 aaattcgatt tcgcgaaact gggatgactg gaagcccgag gccggattca atttgtggcg
     1441 gaaacccaga aaatctctgg gtgccatggt ctcgaggata tgaatttgct gcaccagcac
     1501 tctcatgatg gagactaccc ggcgcataaa atgggtagcc cgacgtacct tatcttctct
     1561 cattagttca acgaccagat ctagctcgtg aattatcaat ttgaaccaga gttcgtaggt
     1621 ctggtgaatg ataatgaaga gcggttcgtc atggtgcgcc ggttctgact ggcaaacctg
     1681 cagttttttg agctcatcga ccatcaggta actattgtag gaaagtggtg tatgaccacc
     1741 agtggcgata ccgccataag aaccgacttc cttcactttg gtctcttttt cgctcattgg
     1801 ttttcttgct cactccactt cttgtttaaa ctagtttagt tgtccttgct ctgcttaggc
     1861 atctttgact taagatctac ctggttctcc ggtgttgccg caggttcggt cggcttggtg
     1921 gagttcttca tgatcttttc ctggggctcc ggttttgcct tgggcggttt ttcttttttg
     1981 ttgcttttgg gtttcttggc cttcttagcc ggcttgagag gtacagcgcc gggcgcaagg
     2041 ggcaagtagc tttgtcgatc caactcttcg ccttctacaa tcttcaagta ggtgagcggc
     2101 ttgcggctgg agtattgaaa ggccagtctg gaagaggcga gttccttctt ggcttttttc
     2161 accagtgctt cataagtgat ttgataagtt tcaaccagga tgccgccggc actgagccgg
     2221 aaaattttga actggcatgg atagacggcc agagaagggc atgagacgta ccagatgtct
     2281 cgctcctttt gaattttgtt ggccccaacg tggccgctca acaccagcct tacgtccctg
     2341 ctcgaaccca gtatttctct agcctgggcg ccttgcgaaa ctatgtattc ctcccagggt
     2401 ggtccactgt catagggagg cggcggcaag aggggatggt ggctggccac aacggtaaac
     2461 ttgccgctgt tctttttcag atcttctttc agccattcca gttgctggct tcgcatctcg
     2521 cccgtattgg aattggctct tgccgtatcc agtccgatca ggtggagccc gcttacggga
     2581 tcgagagacc agtaagtatc ggggctgttg agaccgcggc ctttcagatc gggtccgtag
     2641 gtcctcattt tatctaccgg aatgggaccg gagatatcct gctcgcccat gatgaaatac
     2701 cagggagcat tcaatacttg agctacatct atgaagagtt gccagtgtgt ctcgtctcgt
     2761 cccgggcctt ctacctgat
//
LOCUS       DAOQNZ010000442         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20082_length_2772_cov_3.829960, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000442 DAOQNZ010000000
VERSION     DAOQNZ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20082_length_2772_cov_3.829960"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="PKW73_08850"
     CDS             <1..1177
                     /locus_tag="PKW73_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73434.1"
                     /translation="GKISSGFGTASEGGEDVVSSTMTTSMDSDAPKSSASDKPYPWII
                     SPFIDFFFIIGGGALIFFAVNYLYLGWTIPTNLTDPASFWLITIGFLSQHLFADSHNS
                     ATYLRIWGSAEDQARFKFHRTWLAYSTIPMFILGLSVPGLTGTFVYIYLITVYWHYVA
                     QTFGIALIYCYKRGYFMTSVEKEIFRWFMFSMVGWTTIRFLTFRDLSPRNWFGVDIPF
                     WGPLPIVFHNTSRFLFFFMTVAFVVVLVRKYFKEDKIMPWQAFLLIFTVACLGLSKDV
                     ANTMFWLYVPGFFHGSQYLAVCLAYYLKERGMPEGMNTWEISKAAMGAPGMKYMGLVI
                     LSGVFFYVGIPHFFMQIGYNFSMIGGLVLACVNYHHFITDAAIWRLRDPRCRKILLA"
     gene            1195..2346
                     /locus_tag="PKW73_08855"
     CDS             1195..2346
                     /locus_tag="PKW73_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73435.1"
                     /translation="MVSATFGSDSSQPRPQVMDQAATAPYAWVVGPVFDYLFVVGGFF
                     WLIFALQVGWFHWDAPDPKASGLAGVAAYGLLITSTLGTYIFADAHTIATYMRIYSTA
                     ENRERFKLYAYYLPWMSLLFFSLCLMYPRAAGFCVYLHLMWVFQHYVGQTFGISLVYC
                     YKRGYYFKNWERETYRWFMHSFSAFVITRILCFRTLSPEELWGIKLPFYGLPTWLFYT
                     AEVIFFTMMGAFFVMLLRKAILEKQFLPLPAVCLILTIFGIGLSTGFAHSVLWLYGPP
                     FFHGSQYLAVSLSFFLKEKGLQEGRMAEVSRHILVESFKKNGLFYWGMVVSAGMVIYV
                     CIPQVLVNYGFDFVATATIIQACINFHHFVSDGAIWRLRDKTCREILLA"
     gene            2454..>2772
                     /locus_tag="PKW73_08860"
     CDS             2454..>2772
                     /locus_tag="PKW73_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73436.1"
                     /translation="MSKQVLGIACLVIAALVGMAFYSTNVFTERNRFVPAKIFEAAFD
                     ESVARAEELQGVKDDSTQYDVRLKFKYPHEPKPFHEDEYKTALCYEARNWFNEKYPGE
                     KSLQ"
BASE COUNT          537 a          724 c          694 g          817 t
ORIGIN      
        1 gggtaaaata agtagtggat ttggtacggc aagcgaaggg ggagaagacg tggtaagcag
       61 cactatgacc accagtatgg actcggatgc gcccaagagt tcggcatctg ataaaccata
      121 tccctggatt atttcgcctt tcatcgattt cttcttcata atcggcggcg gtgctctcat
      181 cttctttgcc gtcaactatc tctacctggg ttggactatc cccaccaatt tgactgatcc
      241 ggcttccttc tggctgatta ccataggctt tctctcccag catctctttg ccgattctca
      301 caacagcgcc acctacttgc gcatctgggg ctcggcggaa gaccaggcgc gcttcaagtt
      361 ccatcgtacc tggctggcct attccaccat tcccatgttt attctgggtc tttcggtgcc
      421 gggtctgacg ggcaccttcg tgtatatata cctgatcacg gtctactggc attacgttgc
      481 ccagaccttc ggcatcgctc tcatttattg ctacaagcgc ggctatttca tgaccagtgt
      541 cgagaaagaa atttttcgct ggttcatgtt ttccatggtg ggctggacta ccattcgctt
      601 cctcaccttc cgcgatctca gcccccgcaa ctggttcggc gtggatatac cgttctgggg
      661 acctctgccc atcgtcttcc acaacacttc tcgcttccta ttcttcttta tgaccgtggc
      721 tttcgtagtg gtactggtgc gcaagtactt caaggaagac aaaatcatgc cctggcaggc
      781 cttcctcctg atattcacag tggcttgtct ggggctttcc aaagatgttg ccaatactat
      841 gttctggctc tatgtgccgg gcttcttcca tggcagtcag tatctggctg tttgcctggc
      901 ctattacctg aaggagcggg ggatgcctga aggcatgaac acctgggaga tttccaaggc
      961 ggccatgggc gctcccggca tgaagtacat ggggcttgtg atcctttcag gggttttctt
     1021 ctatgtggga attcctcatt tcttcatgca gattggctat aacttctcca tgatcggcgg
     1081 tctggtactg gcatgcgtca actaccatca cttcattacc gatgctgcca tctggcgtct
     1141 gcgcgaccca cgttgtcgca aaattctgct tgcttgagct gagaggtatt ctgcatggtc
     1201 agcgccacat ttggttccga cagtagtcag ccgcggcccc aggtcatgga ccaggccgcc
     1261 actgctcctt atgcctgggt tgtcgggccg gtatttgact atctcttcgt ggtgggcggc
     1321 ttcttctggc tgatttttgc tttgcaagtg ggctggttcc actgggacgc tccggatccc
     1381 aaggccagcg gactggccgg ggtagcggcc tatggtcttc taataaccag caccctgggt
     1441 acttacatct ttgccgatgc ccacaccatt gccacttata tgcgcatata ttccacggcg
     1501 gagaatcgtg agcgcttcaa gctctatgct tattacctgc cctggatgag cttgttgttc
     1561 ttttccctct gtttgatgta tccccgggct gccgggtttt gtgtttattt gcatttgatg
     1621 tgggttttcc agcactatgt cggacagacc ttcggtattt cacttgtcta ctgctataag
     1681 cgcggttatt acttcaagaa ctgggagagg gaaacctacc gctggttcat gcacagcttt
     1741 tctgcttttg taatcacccg catcctctgt ttccgcaccc tttctccgga agaactctgg
     1801 ggtatcaagc ttccctttta cgggttgccc acctggcttt tctacacggc agaggtgatc
     1861 ttcttcacca tgatgggtgc tttcttcgtc atgctcctgc gcaaggccat cctggaaaaa
     1921 cagttccttc ctctgccggc cgtctgtctc atcttgacta tcttcggcat cggcttatct
     1981 acaggttttg cccattccgt actgtggctc tacggaccgc ccttcttcca cggcagtcaa
     2041 tacctggcgg tttccctcag tttcttcctg aaagaaaagg gtcttcagga gggacgtatg
     2101 gctgaggtca gtcgtcatat cctggtggag agtttcaaga agaacggtct cttttactgg
     2161 ggcatggtgg tgagcgccgg tatggtcatt tacgtctgta ttccacaggt acttgtaaac
     2221 tacggtttcg atttcgtagc cactgcgacc ataattcagg cctgtatcaa ctttcaccac
     2281 tttgtatctg atggtgccat atggcgcttg cgggacaaga cttgccgaga aatcttattg
     2341 gcctgagaat atttggggca ctaaagaggt gggcatttgc aggcagcggc gagtattttc
     2401 gtcgtataat ctgctttatt ggtttcaagt tatctctaaa gtgagtatca agggtgtcta
     2461 agcaagttct cggtattgcc tgtctggtga ttgcagcctt agtcggcatg gctttttatt
     2521 ccaccaatgt cttcactgaa cgtaacaggt ttgtgccggc caaaatattc gaagcggctt
     2581 tcgacgagtc ggttgctcgg gctgaagaac ttcagggcgt taaggacgac agtacgcagt
     2641 acgatgttag gctcaagttt aagtatcccc acgagcccaa gccctttcat gaagacgagt
     2701 ataagaccgc tctttgctat gaggcgcgca actggttcaa tgaaaaatat ccaggtgaaa
     2761 agtcgctgca gg
//
LOCUS       DAOQNZ010000443         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20088_length_2772_cov_3.128450, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000443 DAOQNZ010000000
VERSION     DAOQNZ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20088_length_2772_cov_3.128450"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="PKW73_08865"
     CDS             complement(<1..2157)
                     /locus_tag="PKW73_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73437.1"
                     /translation="MPSKIASCVSLAFLVAAFLSDSALARRAYSTAEAEGEAIGAVKP
                     VSVAPVRKPKRRKARAKLATSGSAEAGADSSAPASDNKHSSGSTEVGSAGINKAASAG
                     GSGSPLAGSPGSGSEGKGAVSLSKLLSEAEEAARAENYDQCTALMNDCLKALTADGSA
                     RGFSPDAAAPAEPRETVDKAGAAGTAETLKQMAAVYSLMERPDAAARCMEEALRLNPR
                     EPALLAQAVECLSNEGQFANACRLAEDYLEGHRNKNLSRALALTQARMGEFEKALATI
                     NSGFGGTGRNNTEVSEVKAEILSAVGDYGEALDVYEGLERNNPEEVFYPTSAAELLRK
                     LGKFDQGLSRCDAALKLAPLDPAALACRVRILLDLRRNEEAVKAVTDLVRVAPDSGNY
                     LLAAEVFDASAAYDRGLKAVEDGIAKLGHLPELELARVKELINLGRGEGAIDDALAEE
                     PGRLSYQLLKLRMLIKSGQLAEAQKLGAKLKASSGWTVSTRLGLAQLAMVQGHYMPAA
                     RALESALRLLPRGDQRRYELELLLAANYRLAGEKALAQKTLSALEQEVESTVEGGYPA
                     SLVASLLAADGTAAAKGEGRLQQVQSDFYRGLRLLADGKTDAGRDLLQSAQKLAAPSS
                     SEAALLPRLLEGPGKASFLTGWLAGVPGWNYLLPVMLTIWSLVGGGIFLYKYRRRDET
                     KRVERSDVRVGRPHPQAVASPSPPKPLQQQPAIETPV"
     gene            complement(2274..>2772)
                     /locus_tag="PKW73_08870"
     CDS             complement(2274..>2772)
                     /locus_tag="PKW73_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA73438.1"
                     /translation="VPLAMLGALLAQWLRHLDNDVFCQIGLVMLVGLASKNAILIVEF
                     ANHLLKTENASLETAIKKACEIRFRPILMTSLAFVMGILPLVFATGAGAHSRHSLGTA
                     VCGGMIVSTVLSLYIVPVIFLVVRGITDRAGRRPAGVSSPDNDSAPDPLAPEPLESKN
                     GDGEV"
BASE COUNT          625 a          845 c          757 g          545 t
ORIGIN      
        1 gaccggtgtt tcaattgcgg gttgctgctg taaaggtttc ggaggtgaag gagaggcaac
       61 tgcctgcgga tgcggtctgc cgacacgcac atccgaccgc tccactctct tggtctcgtc
      121 ccgtcttcgg tacttgtaga gaaaaatgcc gccgcccacc agagaccata tggtcagcat
      181 aaccggcaaa agataattcc agcccggcac cccggcaagc catccagtca ggaaactggc
      241 ttttcccggc ccttccagaa gtctgggtag caaagccgcc tcactggaag atggtgctgc
      301 taacttctga gccgattgca gcaaatcccg gccggcgtcg gttttaccgt cggccagtag
      361 gcgcaaacct ctatagaaat cagactgcac ctgttgcaaa cgcccctcac ctttagcagc
      421 ggcagttccg tcggccgcca gcaaagaagc caccagcgag gctggatagc cgccctccac
      481 cgtggactca acttcttgct ccagggcgct caaagtcttc tgggcaagcg ctttttcacc
      541 tgccagtcga tagtttgccg ccagcaagag ctcaagctcg tagcgcctct ggtcaccgcg
      601 aggcaggagg cgcaaggctg actccagagc cctggcagcg ggcatgtaat gtccctgcac
      661 catggccaac tgtgcaagcc ccagccgcgt ggagaccgtc caaccactgg aggctttcag
      721 tttggcgccg agcttctggg cctcggcaag ttgcccgctc ttaattaaca ttctcagctt
      781 gagcaactga taactgagcc gaccgggctc ctctgcaaga gcgtcatcaa tagcgccttc
      841 accgcgaccc agattaatca attccttgac acgggccagt tcaagttcag gcagatgtcc
      901 cagcttagcg attccgtctt ccacagcctt gagcccccgg tcataggcag cagaagcatc
      961 aaagacttcg gcagcaagca gatagttacc gctatcggga gcaacccgaa ccagatcggt
     1021 gacagctttg accgcctctt cattgcggcg gagatcaagg agaattctca cccgacaggc
     1081 aagagccgca gggtcgaggg gagcaagttt caacgccgca tcacaacggc tcagaccttg
     1141 atcgaacttg cccaattttc taagcaactc tgcggcactg gtcggataga aaacttcctc
     1201 tggattgttg cgctccagcc cttcgtaaac atcgagagct tcaccataat cgcccacggc
     1261 tgagagtatt tcagccttga cctcactcac ttccgtgtta ttgcggccgg tgccgccaaa
     1321 gccggagttg attgtggcaa gcgccttttc aaattcaccc atgcgcgcct gggtaagggc
     1381 cagagcccgc gaaaggttct tgttgcgatg tccctcaaga taatcttcgg ccaggcggca
     1441 ggcattagca aactgtcctt cattgctcaa gcactccacc gcttgggcaa gcaaagccgg
     1501 ctcgcgagga ttaaggcgca gagcctcctc catacagcgg gcggcagcgt cgggacgttc
     1561 catcaaagaa tagacggcag ccatttgttt gagagtttcg gcagtgccgg cggcaccggc
     1621 tttgtcgacg gtttcccgag gctccgccgg ggcggcggcg tcgggagaaa agcctcgggc
     1681 agagccatcc gcggtcagcg ccttgaggca atcgttcata agcgccgtgc actgatcata
     1741 attttcagcc cgggcggctt cctccgcctc gctgagcagt ttggaaagac taacagcgcc
     1801 cttgccctcg ctgcctgaac cggggcttcc agccagaggc gacccggaac caccagccga
     1861 ggcagctttg ttgataccgg ccgagccgac ttcggtcgat cccgaagagt gcttgttatc
     1921 agacgcagga gctgaggagt ccgccccggc ctcggcagac ccggaggtgg ccagtttagc
     1981 tctggctttg cgccgtttag gctttctgac aggagcgaca ctgaccggct ttacggcacc
     2041 gatagcctca ccttccgctt cagccgtgga ataagcccgc ctggccagtg ccgaatcgga
     2101 aaggaaagcg gcaaccagaa atgcaagact gacacatgat gcgatcttgg aaggcaatcc
     2161 catttctcaa cccgattctt agagataccc cagtgccttg ccccagcaga ttcaccaatt
     2221 ttagcagagc cactgcagag ccctgccgct acatccggca gcggcgattt cgctcagact
     2281 tccccgtcgc cgttcttgct ttccaaaggt tcaggggcaa gcggatcggg cgccgaatcg
     2341 ttgtccgggg aggagacccc ggcaggtcgc cgtccggctc tatcggttat gccgcgcacc
     2401 accaggaaga taacaggcac gatgtagagg ctgagcacgg ttgaaacaat catgccgccg
     2461 cacacggccg taccgagaga atgccggctg tgcgcaccgg caccggtggc aaagaccagg
     2521 ggcaatatac ccatgacaaa agcgagagat gtcatcagaa taggacggaa acgaatttca
     2581 caggctttct tgatggccgt ttccagagaa gcattctcag tttttagcaa gtgattggca
     2641 aattccacaa tcaaaatggc gttcttgctg gccagtccaa ccagcataac cagaccgatc
     2701 tgacagaaaa catcgttatc aagatgccgc agccattgag caagcaaggc gccaagcatg
     2761 gccagcggca cc
//
LOCUS       DAOQNZ010000444         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20156_length_2767_cov_2.401917, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000444 DAOQNZ010000000
VERSION     DAOQNZ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20156_length_2767_cov_2.401917"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PKW73_08875"
     CDS             complement(<1..1013)
                     /locus_tag="PKW73_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNA73439.1"
                     /translation="MESQYSPKEFDEKWQKHWEALELYKAHDDSSKPKFYSLVMFPYP
                     SGDLHMGHMRVYTISDVISRQRRMLGYNVLNPMGWDAFGLPAENAAIKRKLHPEKWTK
                     ENIRFMRDEQLKKLGTSYDWQREVTTCDSNYYHWTQWLFLKFYEKGLAVRKEAPVNWC
                     PDCQTVLANEQVEGGACWRHPETPVEQKQMSQWFLRITHYAEELLADLDTLKGWPERV
                     RLMQQNWIGKSQGAELYFTVESKPNVQIKVFTTRPDTVFGVSYVVLAPENPLVNELVS
                     DDCREAVCAYVEEAKRKTELDRMTTEKSKSGVFIGAHVVNPFNGDVVPIYVSDNVLMS
                     YGTG"
     gene            1351..2658
                     /gene="tyrS"
                     /locus_tag="PKW73_08880"
     CDS             1351..2658
                     /gene="tyrS"
                     /locus_tag="PKW73_08880"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNA73440.1"
                     /translation="MNADLNLEKNTAQGAQDGSKQKVDASGQPEIPQDILAEAAELCR
                     GAEVLPDGVNGLARKIWQARQEGRKLRAKLGVDPTSTDLHLGHAVVFRKLRRFQDFGH
                     QVVLIIGGFTAQIGDPTGRNSTRPPLNEQEVNANAQTYLNQMGLILDLDKTEVVNNNN
                     WLAPLTMKEVIKLAASVTANQLLAKEAFGERLDKQQPVAFHELFYPLLQAYDSVAIQA
                     DVELGGTDQRFNILQGRELQPKYGQTPQMAMLLPLLEGTDGVKKMSKSYGNYIGLKEP
                     AEEIFGKCMRITDDLILKYFELTTTLSGEEVDAIERAHLQDGGNPKDAKLKLAQQVVR
                     QYHGAEAAQRALENWQKVHSEGQMPEDMPVFEVAAEEAVFRLLTMSKLVASNGEGKRL
                     VQEGGVKLDGETVKDPNQLIKLAAGQSHVLQVGRRKFVKLLGK"
BASE COUNT          706 a          768 c          730 g          563 t
ORIGIN      
        1 ccggtgccgt agctcatcaa gacattgtcg gagacgtaaa tcggtactac atcaccgtta
       61 aagggattga ccacatgggc accaataaag acgcctgact tggacttctc agtggtcatg
      121 cgatccagct cggttttacg cttagcttct tccacgtaag cgcagacggc ttcccggcaa
      181 tcatcactga ccagttcgtt caccagcgga ttttccggtg ccagcaccac atagctcaca
      241 ccaaatacgg tgtcaggtct ggttgtaaaa accttgatct gcacattggg cttggactcg
      301 accgtaaaat atagttccgc cccttgagac ttgccgatcc agttctgctg catgaggcgc
      361 acccgctcag gccaaccttt gagcgtatcc aggtcggcga gcaattcctc ggcataatga
      421 gtaatgcgca agaaccactg gctcatctgc ttctgttcca ccggcgtttc aggatggcgc
      481 cagcatgcgc cgccttccac ctgctcgtta gccagtacgg tctgacaatc aggacaccag
      541 ttgaccggcg cctctttccg cacggccaga cctttctcgt agaacttcaa gaacaaccac
      601 tgggtccaat gataatagtt gctgtcgcag gtagtcactt cccgctgcca gtcataactg
      661 gtaccgagct tcttaagttg ctcgtcgcgc atgaagcgaa tgttttcctt ggtccatttc
      721 tcagggtgca atttacgctt gatggccgcg ttctccgccg gcagtccaaa agcgtcccac
      781 cccatggggt tcaacacgtt gtaaccaagc atgcgccgct gacggctgat cacatccgag
      841 atagtgtaaa cacgcatgtg ccccatgtga aggtcaccgg acggataggg aaacatgacc
      901 agggaataga atttgggctt agagctatcg tcgtgagcct tgtacagctc gagggcttcc
      961 cagtgcttct gccacttttc gtcgaattcc ttcggactgt attggctctc catctaattc
     1021 aaatccccgc aagctgtaaa atcgacactt gactttgctc agccccgcct gggcatgcga
     1081 caaagtcata ccggtcagga cctaacactg ctgccagtac tcgcaaaaat cttacttgga
     1141 aagcgcaagc gactcaaggg aaagagtaat catgataaga tccttgccgc attgatattg
     1201 gacggcaaaa gcctgccgac cgctgctggt cgaaagaggc tccgaccagc taatgccgtt
     1261 acataaagca acagacgact gtgcccgaga aacgggcagt tgaatgtgct gacggaagct
     1321 tcagacccca gccaagcgaa ggaaatcctc ttgaacgcag acctcaatct agaaaaaaat
     1381 accgcccagg gcgcccagga tggaagtaag cagaaggttg acgcgagcgg tcagccggaa
     1441 attccccagg atattctggc ggaagcggct gaactctgcc gcggggcaga ggttctgccg
     1501 gatggagtga acggtctggc gagaaaaatc tggcaagctc gtcaggaggg acggaaactg
     1561 agagccaagc tgggcgtgga tccgacatcc accgacttgc acctcggtca tgcggtggtc
     1621 ttccggaaac tacgccgctt tcaagacttc gggcaccagg tggtgctgat catcggcggc
     1681 ttcaccgccc aaataggtga ccccaccggt cgcaacagca ccagaccacc gctcaatgag
     1741 caagaagtga atgccaacgc ccagacctac ttaaaccaga tgggtttaat cctggacctg
     1801 gacaagacgg aagtagtgaa caataacaac tggctggctc ctctcaccat gaaggaagtc
     1861 ataaagctgg cggcatccgt cactgccaat caattgcttg ccaaagaggc ttttggtgaa
     1921 agactggata aacagcagcc cgtggcattt catgagctgt tctaccccct cttgcaggcc
     1981 tacgactcgg tggccataca ggccgatgtt gaactgggcg gcaccgacca gcgctttaac
     2041 attttgcagg ggcgcgaatt gcagcccaaa tacggtcaaa cgccgcaaat ggccatgctt
     2101 ttgccccttc tggagggcac cgacggcgtc aagaagatgt ccaagtctta cggcaactac
     2161 atagggctga aggagcccgc cgaagaaatc ttcggcaaat gcatgcgcat caccgatgac
     2221 ctgatcctca agtactttga attgacaacc actttatcag gagaggaagt ggacgccatc
     2281 gagcgcgccc acttgcagga cggtggcaat cccaaagacg ccaagctgaa actggcccag
     2341 caagtggtgc gccagtatca cggcgccgaa gcggctcaaa gagcactgga aaactggcaa
     2401 aaggtacact cggaagggca gatgcctgag gacatgccgg tttttgaagt ggcagcggaa
     2461 gaagctgtct tccggctcct gaccatgagc aagctggtgg cctccaacgg tgagggcaaa
     2521 cgcctggtgc aggaaggggg cgtcaaactg gacggcgaaa ctgtgaaaga tcccaatcaa
     2581 ctgataaagc tggctgccgg gcaaagccat gtactccagg tgggcagacg caaatttgtt
     2641 aaactcttgg gaaaatgaaa tccggcaaat aaagttgggc gaatcatgcc ggttaacttt
     2701 caaaaacccg ccaaccctgg tccggtctcg aattcgtagc atccccacgg cagcttaaaa
     2761 aagccgg
//
LOCUS       DAOQNZ010000445         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20205_length_2764_cov_3.192691, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000445 DAOQNZ010000000
VERSION     DAOQNZ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20205_length_2764_cov_3.192691"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="PKW73_08885"
     CDS             complement(<1..1752)
                     /locus_tag="PKW73_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73441.1"
                     /translation="MTMLAKWIRKATNLLSEPDKNELKADDFAIEHVLFYHGLVGSSP
                     ESGRAFKDVRADFHSAVARNNSQNLCICLRLPPLHWEDKLKKIVDEIKGKSREDLIQT
                     LLPPDYHDRDKESHDPLHHSDWQVRANAALILAHIGAEQAVDRLIASLEDTAINTSPA
                     FCHIARSLAALRTETAHQALISYADHSEPWIRVDAVSALSTWPLEKIKEPLARSFASH
                     HQFFDYQSVVVARHYRPVEFIKGTEQDKINEQTGDIGLEIAIGLVEAFRSTFAGQKSM
                     AAELGLPQVAAVLSQTELSSPLAARTLHQVLTWLEENDDLNDDGIDWPSEAQLKAGQE
                     NLQAEETTQAVLAVLQEALKMAPQTQAESASVSKVRHAVKLAGELKIAAAYDELIQLL
                     DTGPAYRNEAVEALGRLGKAEAATALVKLAGQCVKMEARTGLPLSPQPIQEKDARAAE
                     TYWHILQALGNIPTTEALNLLLEAANDSAPDKRQAALDSAIKLFRKTNGQLARSNEVK
                     TAIIKSLNDPSTAVRTMAAQACAPMNLSDAILPLSKLALAGEISLARAALDSLSELAA
                     AGEKEKVVAALTEAARAE"
     gene            1930..>2764
                     /locus_tag="PKW73_08890"
     CDS             1930..>2764
                     /locus_tag="PKW73_08890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA73442.1"
                     /translation="MNTFLSAAQKTLQEQYVKFVDEVIAPVAAELDKVEGSSCQKEIM
                     SKLGQNGYLGITVAKDLGGQGGTLLDAALFVEALGAQAAGLALAVSSHYSVVELISRF
                     GSETQKSRYLPLLARGECFGAQAFSEEKAGSDLSLIETIAEVNGAEVKLTGVKTWVVS
                     ADLPVLLAVLGKNGEEKGVWLLDGADRSSVELSARKGTMGFRSARLFDVTLKAAKASA
                     DNQLKGADALALASCALDVSKTLLAACALGLCQQGLSLAAQRANTREQFGAPIAKNQG
                     VQ"
BASE COUNT          510 a          701 c          872 g          681 t
ORIGIN      
        1 ttccgccctg gcggcctccg tcagcgctgc cacaaccttt tccttttcgc cggcggcggc
       61 cagttcactg aggctgtcga gggccgcacg agccaggctg atttctccag ccagggctag
      121 tttggaaagg ggaaggatgg cgtcgctgag gttcatggga gcacaggctt gggcggccat
      181 ggtgcgcacg gcggtggaag ggtcgttgag gctctttatg atggcggtct tcacttcgtt
      241 ggagcgggcg agctgtccat ttgttttccg gaaaagtttt atggctgaat ccagagctgc
      301 ttgccgcttg tcgggggcgc tgtcgtttgc ggcctccagt agcaaattga gggcttcggt
      361 ggtggggata ttgcccaggg cctggagaat gtgccagtaa gtttcggctg cccgggcatc
      421 tttttcctgg atgggttgag ggctgagggg cagtcctgtg cgggcttcca ttttgacgca
      481 ctgtccggcc agtttgacca gagctgtagc cgcttcggct ttgcccagcc ggcccagagc
      541 ctccactgct tcattgcggt aggcggggcc tgtgtccaga agctgtatga gttcgtcata
      601 ggcggcggcg attttgagtt ctccggccag tttgacggca tggcgaactt tgctcaccga
      661 ggcgctttcc gcctgggttt gaggcgccat tttgagagct tcctgtaaga ctgccagcac
      721 ggcctgagtg gtttcttccg cctgtagatt ttcttgtccg gccttgagct gagcctcgct
      781 tggccaatca atgccatcgt cattcaagtc atcattttct tccagccagg tgagcacctg
      841 gtgtaaggtt ctggcagcca ggggggagga gagttctgtc tggctcagga cggcggcaac
      901 ctggggcaga cccagctcgg ccgccatgga tttttgtccg gcaaaagtag agcggaaggc
      961 ttccaccagt ccaattgcta tttcaagacc gatatcgcct gtctgttcat tgattttgtc
     1021 ttgctctgtg cctttaatga actcgaccgg gcgatagtgt ctggctacaa ctaccgattg
     1081 gtagtcgaaa aattgatggt ggctggcaaa agacctggcc agcggctcct tgattttctc
     1141 cagcggccag gtggaaagag cgctgacggc atctactcta atccatggtt cgctgtggtc
     1201 ggcatagctg atcagggcct gatgcgcggt ttcggtgcgc aaagcggcca gggaacgggc
     1261 gatgtgacag aaagcggggc tggtattaat ggcggtgtcc tcgaggctgg cgatcaatct
     1321 gtcgacagct tgttcggcgc cgatgtgagc aagaatcaat gcggcattgg ctctcacttg
     1381 ccagtcggag tgatgaaggg gatcatgact ttctttatcg cgatcgtggt agtcgggcgg
     1441 caagagggtt tgaatcagat cttcccggct cttgccctta atctcgtcta cgattttctt
     1501 cagtttgtct tcccagtgaa ggggcggtag ccgcaggcag atgcagagat tctgagagtt
     1561 gttgcgagca actgccgagt ggaagtcggc ccgaacatct ttgaaagcgc ggccgctctc
     1621 cgggctggag cccaccagtc cgtgatagaa gagcacatgc tcgatggcaa agtcgtcagc
     1681 ctttagctca tttttatccg gttcggagag gagatttgtg gctttacgaa tccactttgc
     1741 aagcattgtc atctgttctg cttccgttat tggggagagg ttgtaggttc cgggtgcggt
     1801 cttgtctggc ggattggccg gtttttaagg gggcggcggg attgctacca ggcccacgga
     1861 tagtgctatg atctggggct tatctggctt tcgatgccaa tatcctagta tgaattcgtg
     1921 ggggtttccg tgaacacctt cttaagcgcc gcacaaaaaa cgctgcaaga acagtatgtc
     1981 aagtttgtag atgaagtaat agcgcctgtg gccgctgaac ttgataaggt cgaaggcagc
     2041 tcgtgccaga aagaaattat gagtaagctc ggtcaaaatg gctacctggg cattacggta
     2101 gctaaagatc tgggcggtca gggcgggacc cttctggacg ccgccctttt tgtggaagct
     2161 ctgggcgcac aggccgccgg tctggccctg gcggtgagca gccactacag cgtagtggag
     2221 ttgatcagcc gcttcggcag cgagactcaa aagtcgcgtt atttgcccct tcttgcacgt
     2281 ggtgagtgtt tcggcgctca ggccttttcg gaagagaaag ccggctccga cctcagtttg
     2341 attgagacca ttgccgaagt gaacggcgcc gaggtcaagc tcacgggcgt taagacctgg
     2401 gtggtcagtg ctgatctgcc ggttctattg gctgtgcttg gcaaaaacgg tgaagaaaag
     2461 ggagtctggc tcctggacgg cgccgaccgg agcagcgtgg aactttccgc gcgcaagggc
     2521 accatgggtt tccgctcggc acgcctgttc gatgtgacct tgaaagcggc taaggcgagc
     2581 gccgataatc aattgaaagg ggctgatgcc ctggctcttg cctcttgtgc ccttgatgtc
     2641 tccaagaccc tgttggctgc ctgcgccctc ggtctctgcc agcaaggtct cagtctggcc
     2701 gctcagcgcg ccaacacccg ggaacagttc ggcgcaccca tcgccaaaaa tcagggtgtg
     2761 cagt
//
LOCUS       DAOQNZ010000446         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20258_length_2760_cov_3.876525, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000446 DAOQNZ010000000
VERSION     DAOQNZ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20258_length_2760_cov_3.876525"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..925
                     /locus_tag="PKW73_08895"
     CDS             143..925
                     /locus_tag="PKW73_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73443.1"
                     /translation="MEPVSNLPEWERRLFQPGGGDAFLFYVVFGQFHQPMTISGSHYK
                     TAGLPKQVTLGLYERENEPQTLESFVQGFLAQKLKEEEPEIFAAVTEAPQCLVISGTV
                     EDPDNLNYLRDTLGVISAMLDNGGVAVYDPQKFHWWSPKHWKETIQRPKDPALNQHVS
                     ILISLENEKSGDKNGPVWLHTRGLRKFGRPDLSLRNLDQSRLDAGVDLCNYLIEDQIM
                     GLQLCEGQTLDAPGLPIGITCHPTGNLEDPDFNNVHIEIRLP"
     gene            1111..1632
                     /locus_tag="PKW73_08900"
     CDS             1111..1632
                     /locus_tag="PKW73_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HNA73444.1"
                     /translation="MSTLVVIGYDDIHKAQEMRIHLLKLQRDYLIDLEDAVVVERDAK
                     GKIKLHQMIPMTAMGAMQGGFWGTLIGCLFFAPIFGMAVGAAGGAISGALADVGINDD
                     FMKELGETLTPGKSALCVLIKQMTEDKVLDDLHGTGGKVLKTNLSKEDESKLQEALGT
                     ARKAASETATAAV"
     gene            complement(1743..>2760)
                     /locus_tag="PKW73_08905"
     CDS             complement(1743..>2760)
                     /locus_tag="PKW73_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73445.1"
                     /translation="SILRRCMVLRKVISIAICLGLFFSTLYLLWMTTPLYALQDAAFA
                     AYQHDRENFERRVDIDGFIDDLLADLILRPAESTPGLTSFQVQVSRGAIALVVTGLAR
                     DLKGAVRRSVTLGNLDLPERRLHQLGRVPTGIEFLPIFAPAMAASPAVRPDDVKTLLN
                     VASRAAGSSAERLKRVAHDRMWTYIYKHPETLPGRLLGCHPSQRQEQLRLLLNEYGLT
                     RENFRGLSSCKIFSDDRGGEIARVGFSFFSPKLNGEFVIKLELTKSGMFGDWRLMRIA
                     NVPEIFEQLGLDYYDQIHNLMRYSLAGLSNESVNAEMQGVTEQIKQSDAAKNILQKWK
                     INLK"
BASE COUNT          774 a          731 c          659 g          596 t
ORIGIN      
        1 gttaagcctg cccaggtaac cgaagcaaat ggcaaatctc aagagcaaat tccacaaaac
       61 cttaagctag cccggcgcca gcctctccct gctatattat tgttaaaccg aactagctca
      121 aagaggcctt tagaggaatt gcatggaacc ggtgtccaac ttgccagagt gggagcgcag
      181 actatttcag cccggtggtg gagacgcctt tctcttttac gtagtctttg ggcagttcca
      241 tcagcctatg accatttcgg gaagtcatta caaaacagca ggcttaccca agcaggtgac
      301 actgggactc tatgaaagag agaatgaacc tcaaacgctg gaatccttcg ttcaaggctt
      361 cctggcccag aagctgaagg aagaagaacc ggaaattttt gccgccgtta ccgaggcacc
      421 acaatgcctt gtcatcagtg gtaccgtgga agacccggac aacctcaact acttaagaga
      481 tactctggga gtcatctcag ccatgctgga caatggcgga gtggctgtct atgaccccca
      541 gaaattccac tggtggagtc ctaaacattg gaaagaaacc atccagagac caaaagaccc
      601 agccttaaat caacatgttt ccatcctcat ttctcttgaa aatgagaagt ccggagacaa
      661 aaatggaccg gtctggctcc acacacgagg gctgcgcaaa ttcggcaggc cggacttgag
      721 ccttagaaac ctggaccagt cacgcctgga cgccggtgtg gacctttgca attacttgat
      781 agaagaccag attatgggct tgcaattatg cgagggtcaa acccttgatg ccccgggact
      841 acccatcggt ataacatgcc atccgactgg aaatctggaa gatccggatt tcaacaacgt
      901 gcatattgag atccgattac cttgatccgt tcccatactg aagccgaatt gaacaaagga
      961 ttaaacccgg caagagtata taaaacacac cagaaatcaa cataagacaa ccggtcaagc
     1021 tgatatactc tagccgacac tggtaaaaac tattaagccc tgaccctgag acctttgtct
     1081 ctcggttggc aattaactgg aggcgttttc atgagtaccc tggttgtaat tggctacgac
     1141 gacatacaca aagcccagga gatgcgcata catcttctca agctgcaaag agactatttg
     1201 atcgacctgg aagacgcggt ggtggtggaa cgtgatgcca aaggcaaaat caaactgcac
     1261 cagatgatcc ccatgactgc catgggtgct atgcagggcg gattctgggg cactctcatt
     1321 ggctgcctct tcttcgctcc aattttcggc atggccgtag gtgccgccgg cggtgctatt
     1381 tccggcgctc tcgccgacgt tggcatcaat gatgatttca tgaaggaact gggtgaaacc
     1441 ctgactcccg gcaaatcagc actttgtgtt ctaatcaagc aaatgacgga agacaaagtt
     1501 ctggatgacc tgcatggaac cggcggcaaa gtgctgaaga ccaatctctc aaaagaagac
     1561 gagagcaaac tgcaagaagc tctgggcacc gcccgcaaag ccgcaagcga aacagccacg
     1621 gcagccgtat agatagactc aaaaaaagaa agacgaagtc ggacatgttc cggcttcgtc
     1681 tttctttctg caaccggagc tagaatacag aagagccgac gccaccactt gcggtgccgg
     1741 ctttatttca gattaatttt ccacttctgc aaaatattct tagccgcatc gctttgctta
     1801 atttgctcag tcacaccctg catctccgca ttgacacttt cgttactcaa tccagccagg
     1861 gaatatctca taagattatg tatttgatcg tagtaatcga gaccaagctg ttcaaaaatt
     1921 tccggcacat tggcaatacg catcaagcgc cagtcgccaa acatgcctga cttagtgagc
     1981 tccaacttga tcacgaactc accattcagt ttggggctga aaaatgaaaa acccacccgg
     2041 gcaatttcac caccacgatc atcactaaat atcttacaac tgctcaaacc ccgaaaattt
     2101 tcacgagtca gaccatattc gtttaatagc agacgaagct gttcctggcg ctggctggga
     2161 tgacaaccga gcagtcgccc gggtagcgtc tccggatgct tatagatata ggtccacatc
     2221 cgatcatggg cgactcgctt cagtcgctcg gctgaactgc cggcggcgcg actggcgaca
     2281 ttcagaagag tctttacatc atccggtcga accgcagggg aagccgccat ggccggggca
     2341 aatatgggca aaaactcgat gcctgtcggg acccgaccaa gctgatgtaa ccttctctca
     2401 ggcaaatcca gatttcccaa ggttacagac ctgcgcacag cgcctttgag atcccgggcc
     2461 aaaccggtta ccaccagggc gatggcacca cggctgacct gaacctgaaa acttgtcagg
     2521 ccgggcgtgg attcagccgg tcggagaatc agatcggcga gaaggtcatc tatgaatccg
     2581 tcaatatcaa ccctgcgctc gaaattctcg cggtcgtgct ggtaggcggc aaaagccgcg
     2641 tcctgaagtg catacaaggg agtcgtcatc catagcaaat acagggtcga gaaaaacaaa
     2701 cccaggcata tagctataga aatgactttg cgcaacacca tacaccgacg caagattgag
//
LOCUS       DAOQNZ010000447         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20359_length_2753_cov_2.743884, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000447 DAOQNZ010000000
VERSION     DAOQNZ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20359_length_2753_cov_2.743884"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1563
                     /locus_tag="PKW73_08910"
     CDS             301..1563
                     /locus_tag="PKW73_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNA73446.1"
                     /translation="MELSENNQPASALIAVVGAGTMGASIAVAFAQSGYQVLVKEAND
                     DFLKKGFENIDRLLAGRRKKGLSEEESAEIRARIAGATDYEGFADVELVIEAASENIE
                     IKKAIFTELDEWCNVDAVLASNTSSLSISQIGSFTRRADKVVGMHFFNPAHIMKLVEV
                     IPGLETSHETVRYALELATSIGKLAVRVEECASFLVNRLLGRYMNESLFALTAGIATV
                     EEIDKAVSDYVMPIGPLALRDMNGADIGLAVASYNYNEYGPRFAVPALLSEMVEASVL
                     GQKTGGGFYAYDKETRKRAGVNPALEPLLTKALAKDPTCEVLKEFCNKENLAESLFLS
                     MINEAFLALQERICMPEDLDPALMAGLGMRSGPLELASSMGLPVCLAELERRSTICAD
                     KGAFSAAERFRPAPLLKRFVWAGRSSVF"
     gene            2101..>2753
                     /locus_tag="PKW73_08915"
     CDS             2101..>2753
                     /locus_tag="PKW73_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73447.1"
                     /translation="MTSYRKTRVVQGAVLAFLLSINLPALAIVSTASYDLSSLETTVL
                     GRPQNELSSLKRLEKLELKLFGKTSQGPESERLKAIAGAVSYGKSGGSLTAQAGRLDK
                     VVPEAAPLAEVDDGSEALAEALSLYNQGFKSQAQSKLTALSYSHPGFDVYFNLGVIAE
                     TDGKLNEALNYYERARSFKKDDPEVIEAVVSLKSKLAATPKTTDSNWTAAAPPVAPA"
BASE COUNT          645 a          701 c          703 g          704 t
ORIGIN      
        1 gtgcatttat tttaacgaag tattgcaaaa aagctacaag tctttaataa attccaagat
       61 gtcaagtgat taattgtaga gccctgagac ccttgcctgg tctcacttga gaagattcgg
      121 gtcgatgaaa agggtgggtt ggaaggagca aaaccctata tgtgtcggtg atgaacacgg
      181 gccaatatga gcatattcac agagtcactc cggcttgaaa agcacttagt ctgactcttt
      241 aatttcggac cgagagcaag taactttgca tcatcgacta aatgcctctg ggagaattca
      301 atggagctga gtgagaacaa tcaacccgca tcagccttga tagccgttgt cggcgccggc
      361 actatggggg cttccattgc cgtggccttt gcccaaagcg gctaccaggt gctggtgaag
      421 gaagcaaacg acgacttcct caagaaaggt tttgaaaata tcgatcgtct gctggctgga
      481 cgtcgcaaaa aaggtctctc cgaggaagaa tcagccgaga tccgggcccg catcgccggt
      541 gccaccgact acgaaggttt tgccgatgtg gagcttgtca tcgaagcggc cagcgaaaat
      601 atcgagatca aaaaggcgat cttcaccgaa ctggatgagt ggtgcaatgt ggacgccgtt
      661 ttagcctcca atacttccag tctttccatt tctcagattg gcagcttcac cagaagggcc
      721 gacaaagttg tcggcatgca cttcttcaac ccggctcaca tcatgaagct ggtagaggtc
      781 atacccggtc ttgaaacctc ccacgaaaca gtgcgctatg cccttgaact ggcaacttct
      841 atcggcaaac tggccgtgcg agtggaagaa tgcgcatcct ttctggtgaa cagactgctg
      901 ggacgttaca tgaacgagtc gctcttcgcg ctcactgccg gcattgccac ggtggaagaa
      961 atcgataagg ccgtaagcga ttacgtaatg cccataggcc cgttggctct gcgtgatatg
     1021 aatggcgccg atatcggtct ggctgtggct tcctacaatt acaacgaata cggaccgcgc
     1081 tttgccgtgc cggccctgct cagtgaaatg gtggaagcct ccgtgctcgg tcagaagacc
     1141 ggcggcggtt tctatgccta tgacaaagag acacgaaaac gcgctggagt aaacccggct
     1201 ctggaaccgc ttcttaccaa agctctggcg aaagacccca cctgtgaggt gctgaaagaa
     1261 ttctgcaaca aggaaaatct ggcagagtcg ctcttccttt ccatgatcaa cgaagctttt
     1321 ctggctttgc aggagcgtat ctgtatgccc gaagatctcg acccggcctt gatggctggt
     1381 ctgggcatgc gctccggtcc cctggaactc gcctcttcca tggggttgcc tgtatgcctt
     1441 gctgaactgg agcgacgctc caccatatgc gctgataagg gtgccttctc agccgcagaa
     1501 agattccgac cggccccgct tctcaagcgt ttcgtctggg ctggccgtag ttccgtattt
     1561 taagagagag cgactgcttt cagtttcagt cttctttcag ttcgccgccg accttgaact
     1621 tgtcgttaat ccagatggcg aggatggaac aggctgccgt tagtgataat cctaaagcga
     1681 cttccatgat ttttcttcct tgaattttcc tggaaaattc ggtctttctt actctttgcc
     1741 agctacaaaa gattttgctt ttgggctctt ctatcttttg tatatgtatt gatatcatcc
     1801 accctccccg gcaaaggtgt ggttaaggaa atctagatag attgttaagg gcgcctgctt
     1861 tcaagcttcg cttgacaatt attaatgggg ttttaagcct tgacccgggc aggctgactt
     1921 ctttcttcct gtctttggtg tatctatcct gatttgggaa ctacctgggc tttcgcccgt
     1981 catcagatgc cgtagaggtt gcaattgaag cttgttttac agggtggcag tacctttctg
     2041 ccacggcttc ttttgacata gtttgctcaa gcggaatttt cgttgccaga ggtcaaaatc
     2101 atgaccagtt acagaaaaac tcgagtagtg cagggtgccg tcctggcttt tcttttgtcg
     2161 atcaatttgc cggcgctggc tattgtttct actgcctcct acgacctttc ctcccttgag
     2221 accactgttt tagggcggcc tcaaaatgag ctttccagcc tcaagcgtct tgagaaactg
     2281 gaactgaaac tctttgggaa gacctcgcag ggtcctgaaa gtgagcgcct caaggcaatt
     2341 gccggtgctg tttcctatgg caaaagcggc ggcagtttga cagcccaggc cggtcggctc
     2401 gacaaggtag tgccggaagc agctcccctt gccgaagttg atgatggcag cgaggccctg
     2461 gctgaagctc tttcgctcta caatcagggc ttcaaatcgc aagcgcagag caaactgaca
     2521 gcgttaagct acagccatcc cggttttgac gtttacttca acctcggtgt aattgcagag
     2581 accgacggca aattgaacga agctctaaat tattacgagc gggccagatc tttcaagaaa
     2641 gacgaccccg aggtaattga agccgtggtt agtctcaaat ctaaattggc tgcaacaccc
     2701 aaaacaactg actccaattg gacggctgct gcaccccctg tggcgcccgc cat
//
LOCUS       DAOQNZ010000448         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20385_length_2751_cov_3.770772, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000448 DAOQNZ010000000
VERSION     DAOQNZ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20385_length_2751_cov_3.770772"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKW73_08920"
     CDS             complement(<1..753)
                     /locus_tag="PKW73_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73448.1"
                     /translation="MPVLLLCLLTAVLPMEAKLPPVLQSFTDDDRVKDAPEDKVDEKN
                     PEAEIFKVRTTNDEKLQARSEIYFLTDKITSCRDRSLHIPGLDLSTLPRERRETLEQS
                     LAAIRARQLASLLNRRGTSLAILGDNSRALNDLDEAVNFDAEYAPAYNNRAWLRAQKG
                     ELKEALTDVNKALELAPKMAEAYDTRGAINLVLKKTDDAIEDFNVSINCNPRYAEAYF
                     HRSIAHKLMGNLDKYKEDESRAKELEKQALFET"
     gene            1529..>2751
                     /locus_tag="PKW73_08925"
     CDS             1529..>2751
                     /locus_tag="PKW73_08925"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNA73449.1"
                     /translation="MAASAKASSLNQPPLAERMRPRNLDEYFGQTKLLEEGGVLKTML
                     SQSLSSFILWGPPGVGKTTLARIIAGQTESKWIAFSAVTSGIKEIKTVMAEAEAFMNI
                     EGRRTILFVDEIHRFNKAQQDAFLPFVENGTIVLVGATTENPSFEINSALLSRLKVFV
                     MQELGLDDLLGIMKSALSLPERGLGDYGLTASDEVLKMIAVYASGDARTALNSLELAA
                     KLAKKREKTEITEAEIRSSVQQAILKYDKDGENHYNLISALHKSIRNSDIQASIYWLA
                     RMLEAGEEPLFVARRLVRFASEDIGLAAPQALTQAVAAMQTVQFIGMPEGKLALTQAV
                     VYMAMAPKSNAIYTAYKAAAQDALNMMQHPVPLHLRNAPTRLMEDIGYGKGYKYAHDF
                     EQGVADDMECLPRELA"
BASE COUNT          564 a          684 c          751 g          752 t
ORIGIN      
        1 cgtctcgaac agggcctgtt tctccagttc tttggcgcga ctctcgtctt ctttgtattt
       61 atctaaattg cccattagct tgtgggcgat gcttctgtga aaataggctt ccgcgtacct
      121 ggggttgcag tttatggaga cattgaaatc ttctatggca tcatcggtct tcttcaaaac
      181 cagattgatg gcgcccctgg tgtcgtaggc ttcggccatc ttgggagcca gttccagcgc
      241 tttgttgacg tcggtgaggg cttctttcaa ttcacctttc tgggctctca gccaggcccg
      301 gttgttgtaa gcgggcgcat actcggcgtc gaagttgacg gcttcgtcaa ggtcgttgag
      361 ggcgcggctg ttgtcgccga ggatggccag cgaagtgccg cggcggttca ggaggctggc
      421 cagttgtctg gcccgaatgg ccgccaaaga ctgctccaga gtctcgcgcc tttccctggg
      481 cagggtgctg agatcaagcc cggggatgtg caggctgcga tctctgcaac tggttatctt
      541 gtcggtgaga aagtagattt cgctgcgggc ctgtagtttc tcgtcgttgg tggtgcgcac
      601 tttgaagatc tcggcttcgg gatttttttc gtctaccttg tcttcaggcg cgtctttgac
      661 cctatcgtcg tcggtgaagc tttgcagtac cgggggaagt tttgcttcca tgggcagcac
      721 ggcggtgagg aggcaaagca gaagcaccgg cataaccgct tctggcttag gactgtgttt
      781 ctgtcgcttt ttagcgagca tcattcctcc aaactgtaat gagactgccc tggtccttgt
      841 gcttaccgca caacccacat tttgcgccaa ttaggagcgg cttcgccagt ctctttcatt
      901 agtcctagat ttaatcttag cccctaatct gattattttt acagttaatg ttgggcagaa
      961 aacagaaatt tgctcacttt gccctttccg ggctgagtgc cctgtggaat gatgatatta
     1021 gaaaggactc attcattgta ttgtccctga cagataagag gatccttaat tatggaactt
     1081 cgggcccggc cgcccacagg ctagacggct tcacgccgca cttttatgga tggttgcctc
     1141 tggtcggcgc cggcatttgt gctcgggggt gttgagaagt cgccccgctg gaagtgctca
     1201 tcgcaagttt gagcttcagc taatttagtt tctctgtcaa agcttacgcg cgtacgctct
     1261 gtttgtgggt atgcgtggaa ctcaggtttt acttggtcgc acatcagtaa tgagggcttt
     1321 atcaaccgga ggggttgcta gagccctcat ttcgcttttg ccatgtgatt ttggtagtgg
     1381 taatattcaa tgcttccgcg tttccctggc tataagcagt taaatgactc gatttttcaa
     1441 gaacagcaag caatttatta tctggttgat gattttcatc ttccttggca ctctcattcc
     1501 cagcgcctgg gtcatgttcc agtaagttat ggcagcctct gcgaaagcct cgtccttgaa
     1561 ccagccgcca ctggctgagc gcatgcgtcc tcgcaatctc gatgagtact tcggtcagac
     1621 caagcttctg gaagagggag gcgtgctcaa gaccatgctc tctcagagtc tcagttcctt
     1681 catattgtgg ggacctcccg gtgtaggcaa aaccactctg gcgcgcataa ttgccgggca
     1741 aacagagagt aagtggattg ctttttctgc cgtcacctca ggcataaaag agatcaaaac
     1801 cgtcatggcg gaagcggaag cctttatgaa cattgaaggg cggcgcacca tattgttcgt
     1861 agacgagatt catcgcttca acaaggctca gcaagacgct ttccttccct ttgtggaaaa
     1921 cggcaccatt gtgcttgtgg gtgccaccac cgaaaatcct tcctttgaga ttaactcggc
     1981 gcttctctcc cggctgaaag tttttgtcat gcaggaactc ggtcttgatg atctgttggg
     2041 cataatgaag tcggctctca gtctgcctga gcgtggtctg ggcgattatg gactgactgc
     2101 cagtgatgaa gtgctgaaga tgatcgccgt ctatgcatca ggggatgcgc gcacggcttt
     2161 gaactcgctt gaacttgccg ccaaactggc taagaagcgt gagaaaaccg agattaccga
     2221 agcggaaatc agatcttcgg tgcaacaggc aattctcaaa tacgataaag acggcgagaa
     2281 tcactacaat ttgatctcgg cgctacataa gtcgattcgc aattctgata ttcaagccag
     2341 tatctattgg ttggcgcgga tgctggaagc tggagaggag ccgcttttcg tggccaggcg
     2401 gctggtgcgt ttcgcttccg aggacatcgg tctggctgct ccgcaggcgc ttactcaggc
     2461 tgtagcggcc atgcaaaccg tgcaattcat aggtatgcct gaaggcaagc ttgctctcac
     2521 tcaggctgtc gtctacatgg ctatggctcc caagtccaat gccatttaca ctgcttacaa
     2581 ggcggcggcc caggacgcat tgaatatgat gcagcatccc gtgcccctgc atttgcgcaa
     2641 cgcacccact cgcctcatgg aagatattgg atacggcaag ggctacaagt atgctcatga
     2701 tttcgagcag ggtgtggcgg atgatatgga atgtttgccg cgagagcttg c
//
LOCUS       DAOQNZ010000449         2748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20429_length_2748_cov_2.652804, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000449 DAOQNZ010000000
VERSION     DAOQNZ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20429_length_2748_cov_2.652804"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2685)
                     /locus_tag="PKW73_08930"
     CDS             complement(<1..2685)
                     /locus_tag="PKW73_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA73450.1"
                     /translation="MDLNPVAITMARLNTWLNGTKSTAPGEFSMRLSLAGDPIVSAFK
                     VEVSDLLSAPLARGEKFDHVIGCIPQVLHPKPLVLPGKQSVRRGEESETSVEGFSEED
                     LYDLSNYCFEQGILEDRFGLPLIASALEQSQLLLNQGGKVTLVLGGRPGRQAIESMFE
                     RRGLDARLVWMRRIQQADDTDLASLVELEKSYDIRFHFFMSRESDAGESVSAATAVKL
                     LASGRPVYHDLLVYEAVTRFEPHVFDFVRNLSQLHLTSLRKELDFSRLQEERVSFLSR
                     LSQSMLESRALPYPHERGDMSIRELLARYLNGFCYYPVEAADLFIAPSRAELSSIIFK
                     LCTEKQDRILLSSTLSPVYIDAARQAGLDITVGNDDLSELCQLDDLVAPRLVLISPKQ
                     LAFPSPITIKALAQQAAKHPERIYLIDDSEHFLISSHLGANMTLRLAAQEDLPPNLIL
                     LYGLVKNTISPDLQLSFLINAPQSWLSGLDIGAELSYSRIAYPTQLLYQWLFEDLMTF
                     PFSDNGFEDAGIVKEKKAQQVSKLSPLITEIEQDPVFAPKPVDPESAGIIRLDYGEFE
                     AAPPDILIKSLFKGFIDEETHVLPQIVQERICAYVKFTRHVGVSPRRVVLAQGVFPLF
                     GAFIQAMTARLGRPPLVAVPDGSYGPLYPMLKYYGAEIMELPTSVEKAYLISSRDLQG
                     LSPKPDVLWLTQPNNPSGVFFDPEKLRNIVDVCYNEDIYIFSDEIFFLLSDHRLGRWT
                     PSSLSAGSYSQGHYQSKIFMVDGVAKSFASGGMRLGFLLAPDETWAADIQRYTATPPL
                     AFLRAWDGLYSAFLDKSPNHMLDVGKVFTEVESYLMEKRRSLSQHREQLLTLLRRYNL
                     DDGYDTPYRGGLFLLGKLTDRHEKLARNAGLLTNPGEWS"
BASE COUNT          723 a          684 c          783 g          558 t
ORIGIN      
        1 gctccactca ccgggattgg tgagcaagcc ggcatttctg gccaactttt cgtgccggtc
       61 agtcaactta ccaagcaaaa acaatccgcc gcgataggga gtatcgtatc cgtcgtccaa
      121 attgtagcgg cgcaaaagcg tcaggagctg ttctctatgc tgcgaaagac tcctgcgctt
      181 ctccatcaaa taagattcta cttcggtgaa gaccttgccc acatcgagca tgtggttagg
      241 cgatttatca aggaaggcgg aataaagacc atcccaggct cgcaagaagg ccaggggagg
      301 agtagctgtg tagcgttgaa tatcggcagc ccatgtctca tccggagcaa gcaggaagcc
      361 caatctcata ccgccggagg caaaactttt agcgacgccg tcgaccatga aaatcttgct
      421 ctgatagtgc ccctggctgt aggagccggc ggaaagagaa gacggtgtcc agcgtcccag
      481 cctgtggtcg gatagcagga agaaaatttc gtctgaaaaa atatagatat cttcgttgta
      541 gcaaacgtcg actatattgc gcagcttctc cgggtcaaag aagacgccgc tgggattgtt
      601 aggctgggtc agccagagaa catcgggctt gggactgagc ccctgcaaat cgcgggatga
      661 aatcaggtaa gccttctcaa cactggtggg cagttccatt atctcagcgc cgtagtactt
      721 gagcatggga tataagggtc catagctacc atcaggcaca gcaaccaggg gtgggcggcc
      781 aaggcgcgcc gtcatggctt gaatgaatgc accaaagaga gggaagacac cctgcgccag
      841 tacgacccgg cgaggagaca cgcccacgtg acgagtgaac ttgacgtaag cacaaattcg
      901 ctcctgaacg atttgcggca agacatgagt ctcttcgtcg ataaagcctt tgaataaact
      961 cttgatcaaa atatcgggcg gcgccgcttc aaattcaccg taatcgaggc gaatgatacc
     1021 ggcactttcc gggtccacag gcttgggggc aaagactggg tcttgctcaa tttctgtgat
     1081 aagaggggag agtttgctca cctgttgcgc ctttttctct ttcacgatac cggcatcttc
     1141 aaagccgtta tcggaaaaag gaaaggtcat tagatcttcg aagagccatt gataaagaag
     1201 ctgggtggga taagcaatgc gtgaatagct cagttcggcg cctatatcca aaccggaaag
     1261 ccaggactga ggagcattga tcagaaaact cagctgcagg tcggggctga ttgtgttctt
     1321 gaccaggccg tagagaagaa tgagattagg aggcagatct tcctgggcgg ccaggcgcag
     1381 ggtcatattg gcgcccaggt gcgagctgat caagaaatgc tcggaatcat cgatgagata
     1441 gatgcgctcc ggatgtttgg cggcctgctg ggcaagcgcc ttaatggtta taggagaagg
     1501 aaaggctagc tgcttgggag aaatcagcac caggcgcggt gctaccagat cgtcgagctg
     1561 acagagttcg ctcaggtcat cgttacccac ggtaatatca agtccggctt gcctggctgc
     1621 gtcaatataa accggcgaaa gggtggagga aagcaagatg cggtcttgtt tctcggtgca
     1681 caatttaaag atgatggaac tcagctcagc ccgggagggc gcgatgaaca agtcggcagc
     1741 ttccaccgga taatagcaaa agccgttcag atagcgagcc agaagctcac gaatgctcat
     1801 gtcgccgcgc tcatgcggat aaggcagggc tcttgattcc aacatagact gggaaagtcg
     1861 gctcaagaaa ctgacccgct cctcttgcag tctcgagaaa tccaactctt tgcgcaagct
     1921 ggtcaggtgc agttgagaga ggttccgcac aaagtcgaag acatggggtt caaatcgagt
     1981 cacggcttca taaacaagca ggtcgtgata gacaggacga ccgctggcca gaagcttgac
     2041 ggcagtagca gcagaaacgg attcaccggc atcggactca cgggacatga agaagtggaa
     2101 gcgaatatcg taggacttct ccagttcaac cagggaagcg agatccgtgt catcggcctg
     2161 ttgaatgcgg cgcatccaga ctagacgggc atcaagtccg cgtctttcga acatgctctc
     2221 aatggcctgg cgtcccgggc gtccaccgag cacgagggtt actttgccgc cctgattgag
     2281 taaaagctgc gactgctcaa gggcactggc aatgaggggc aagccgaaac ggtcttccag
     2341 aatgccctgc tcaaaacaat aattggagag atcataaaga tcctcttccg aaaaaccctc
     2401 aaccgaagtt tcactttctt cgccccgacg gacgctctgc ttaccgggca agaccagggg
     2461 cttgggatgg agcacctggg gaatgcagcc aatcacatga tcaaactttt cgccacgggc
     2521 aaggggggcg gaaaggaggt cggaaacttc caccttaaag gcgctgacaa tcgggtctcc
     2581 ggccaggctg aggcgcatgg aaaactcacc gggagcggtg ctcttggtac cgttgagcca
     2641 ggtgttgagt ctggccatcg tgatagccac cggattcaaa tccaaaccca ctacttcctt
     2701 tacatgagta aagaagagca aaagcaaaga aatccagcct gagccggt
//
LOCUS       DAOQNZ010000450         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20451_length_2745_cov_4.303717, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000450 DAOQNZ010000000
VERSION     DAOQNZ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20451_length_2745_cov_4.303717"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /locus_tag="PKW73_08935"
     CDS             <1..1579
                     /locus_tag="PKW73_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNA73451.1"
                     /translation="GRSELPPLWALGHQQSRWSYPDAETVRDLAREFRRRRIPCDTLV
                     LDIDYMDDYRVFTSDKGRFPDFKGLIEELARDNFRMVTIVDPGVKLDKDYKIYQEGLK
                     LELFCRDAKGEVFVDRVWPGRSVFPDFQMEATRKWWAEKLQFYYDNGVSGIWNDMNEP
                     AFFDTRFIPVSSFDELPETGEQPFLQRDDADGEPTTVGHLEVRNLYGSMMCEATYNAA
                     LAARPGERPFVLTRSAFAGIQRHAAVWLGDNKSWWEHLRKSLPMLLNVGLSGVPFAGV
                     DIGGFGETCSPEMLLRWYEVGIFFPFFRNHCWLKGRAQEPFAYGAAVEKKARRLIELR
                     YSLLPYISGLFHEHRQTGAPLMRPLLYHYPQDAMALNCHDQFMFGADILVAPVMERAQ
                     THRYVYLPEAGSQWTALASDKTYEGGRSHYLEMPLGTCPAFVREGAILPFADAMQSTA
                     EYEQAAITFKVYGQQGSAVFYEDDGKSFDYKEGGFAQWQLAYSEGFLSRLMGGHYKTS
                     RRYFLQKPDGSKIPVQL"
     gene            1765..2367
                     /locus_tag="PKW73_08940"
     CDS             1765..2367
                     /locus_tag="PKW73_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73452.1"
                     /translation="MTDGYAGAEKTIAWSERLNRAFSSVDTDGDGFLTKGEMQNCLQK
                     EACGLLTGKPEAEAFNKNFDDIQFASNFSTSFKRGLMGREGVARSDLEELARRARELP
                     EAMEKAKDVEAVLSKYYQGSELRKWRFDSLVNSPRVNEADREVLRYVQSNWRHFKVDI
                     GNQFNSDQALIPSRLPQLAEAQRNKLARQYEPVRKVGESL"
     gene            complement(2484..>2745)
                     /locus_tag="PKW73_08945"
     CDS             complement(2484..>2745)
                     /locus_tag="PKW73_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73453.1"
                     /translation="RKAQMQILDAIEFKSRNLSQLAPTEDPEELAVMIQEIWKARKLI
                     DTSSVQGRRLRKEVDRVVEAWLRIYDFLVEEQAASKAEGTAE"
BASE COUNT          583 a          684 c          792 g          686 t
ORIGIN      
        1 cggacgctcg gagcttcctc ccctctgggc cttagggcac cagcagagcc gctggagtta
       61 tcccgatgcc gagacggtgc gggacctggc ccgggaattt cgccggcgtc gcataccttg
      121 cgatactctt gtgctcgata tcgattacat ggatgactac cgagtcttca ccagtgacaa
      181 ggggcgtttc ccagatttta aggggctcat agaggagttg gcccgggaca atttccggat
      241 ggtgaccatt gttgatcctg gcgtaaaact agataaagac tacaagattt atcaggaagg
      301 tctgaagtta gaactcttct gccgcgacgc caaaggagaa gtctttgtag atagggtctg
      361 gcccggtcgc tctgtcttcc ctgattttca aatggaagca acccgtaagt ggtgggctga
      421 gaaactgcag ttctactacg ataacggtgt gagcggcatc tggaacgaca tgaatgaacc
      481 ggctttcttt gatacgcgct tcattcctgt ttcaagcttt gatgagttac ccgagaccgg
      541 cgagcagcca ttcttgcagc gggatgacgc cgatggcgaa ccgacaactg tcggtcattt
      601 ggaagtgcgc aatctctacg gtagtatgat gtgcgaagct acatataatg cggctcttgc
      661 cgccagaccg ggggagcgcc cgtttgtctt gacgcgttcg gcttttgccg gtatccagcg
      721 ccatgccgcc gtctggctcg gggacaataa atcatggtgg gaacatctgc gcaagtcgct
      781 acccatgctc ttgaacgtcg gcctttcagg agtgcctttt gccggagtgg atatcggcgg
      841 ctttggtgaa acctgcagcc ctgaaatgct tctgcgctgg tatgaagtag gcattttctt
      901 ccccttcttt cgcaatcact gctggcttaa aggcagagcc caggagcctt tcgcttacgg
      961 agcggcggtg gagaagaaag cccgtcgctt gattgaactg cgctacagtc ttctgcccta
     1021 tatcagtggt ctcttccacg aacaccggca aaccggcgcc cctctcatgc gtccactgct
     1081 ctatcattac cctcaagatg ccatggccct caattgccac gatcagttta tgtttggtgc
     1141 cgatattctt gtggcccctg ttatggaacg ggcgcaaacc catcgttatg tctatctgcc
     1201 cgaagccggc agccagtgga ctgctcttgc gagcgacaaa acctatgagg gcggtcgcag
     1261 tcattatctg gaaatgcctc tggggacttg cccggctttc gtaagagaag gggcgatttt
     1321 gcctttcgcc gatgccatgc agagtaccgc cgagtatgag caagccgcta tcacctttaa
     1381 ggtctatgga cagcagggca gcgctgtctt ttatgaggat gacggcaaga gcttcgacta
     1441 taaagagggc ggctttgctc agtggcagct tgcctacagt gagggcttcc tgagccgcct
     1501 tatgggtggt cactacaaga caagccgccg ctacttcctg caaaagccag atggttccaa
     1561 aatacctgtg cagctttaat tggcggtgag ggttaaggtt ttgtctgcga ggtgctgtgc
     1621 gactgacgtt gagccggacc gagagctgaa gtttagtact tgtttgaata ttgggagcgc
     1681 tatgccggat attcaagcaa gtttcacgta gggcgcgata ggctaagctt cggagtcaga
     1741 cttggaacct gggaggtcct atttgtgact gatggctatg ccggggcgga gaaaactatt
     1801 gcctggtcgg agagactcaa tcgcgcattc tcttccgtag ataccgatgg cgacggcttt
     1861 ttgacgaaag gggaaatgca aaactgtttg cagaaggaag cctgtggttt gcttactgga
     1921 aaaccggagg ctgaagcctt caacaagaat tttgatgaca tccagttcgc ttccaatttt
     1981 tccacctcct ttaagcgcgg tctgatgggc cgggaaggtg tagcccggtc ggatctggag
     2041 gaattggcga ggcgggccag ggagctaccg gaagccatgg agaaagctaa ggacgtggag
     2101 gcggtgctgt ctaagtatta tcagggctcg gagctgcgaa agtggcgctt cgacagtctt
     2161 gttaattccc ccagggtgaa tgaggctgac cgcgaggttc tgcgctatgt acagagtaac
     2221 tggcgccact tcaaagtaga catcggcaac cagttcaatt ccgaccaggc gctaattcca
     2281 tcgcggctgc cgcaactggc cgaggcccaa agaaacaagc ttgccaggca gtatgagccg
     2341 gtgcgaaaag tcggggagag cctttaggta gagcggcgtg ctcggttcca ttgggagcag
     2401 ctgtcattgg ttgtaggctt aatcggcaga agccttcctt tgttgtaggc ttcatcggga
     2461 gcagccttca tcgttagatg ccgtcactcg gcggtgcctt ctgctttgct ggcggcttgc
     2521 tcctctacga gaaagtcata gatacgtagc caggcttcca ctactctgtc gacttctttg
     2581 cgcaggcgtc ggccttgtac actggaagta tcgatgagtt tgcgcgcttt ccatatttct
     2641 tgaatcatga cggccagctc ctccggatct tctgttggcg ccagttgtga gagattgcgg
     2701 cttttgaatt caattgcatc aagaatttgc atttgcgctt ttctg
//
LOCUS       DAOQNZ010000451         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20473_length_2744_cov_3.911119, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000451 DAOQNZ010000000
VERSION     DAOQNZ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20473_length_2744_cov_3.911119"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PKW73_08950"
     CDS             <1..566
                     /locus_tag="PKW73_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73454.1"
                     /translation="VLIQTGMIAPGVVDESLRLQEMVSSGELSGLQAADILRQANARK
                     VGIDVIMEERASRKEEVEKINSVLEVILQSGALSGENFNRAQSLARQLNVSIAEVILT
                     KEMVEKRLIMAAYQGQSLVNDGIINGAQCVLAMKMCVKTGKEFHEVLKELPSDTAESS
                     PPADDSAGGDKKSGWLGSLWSKVTKKD"
     gene            575..1972
                     /gene="murF"
                     /locus_tag="PKW73_08955"
     CDS             575..1972
                     /gene="murF"
                     /locus_tag="PKW73_08955"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNA73455.1"
                     /translation="MLTFSLDELTSLAASPLVGTLPDDGGLSGAFRGSVCTDSRQIES
                     GDVYLALKGERFDGHRFVKNALDGGAALAVVEASRVESLLKVCQGPLLPVPDTLKYYQ
                     GLARLARRRQKPFVIGITGSSGKTTTKEMCFAVFSASAGKVVHKSAANENNEIGVPKT
                     ILSMPENTDFLIVEMGMRGLGQIDELASTAEPNLGIITGAGSTHIELLGSRENIAKAK
                     CELFAHIDPSSGFAICGDFSPLLKEAAAGVFTGRVCDFDGEDLQVRRITATGTSFSLG
                     SEEFFVQAHGDFLLRDAWCAVKAGLCAGLTPETIRQGLSNWRAVEGRGNVIATGSGLT
                     LIDESYNANPDSVRCAVESVLACEAYQGKRKIIVLGKMAELGDFTEALHRELGSWLKT
                     KAIDCLVTVGAEAGMIASEAGGAAFEVMPCADQEEAHRRLRETISQGDCIMVKGSHST
                     NLEALVRRLEQVVGD"
     gene            2001..2576
                     /locus_tag="PKW73_08960"
     CDS             2001..2576
                     /locus_tag="PKW73_08960"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNA73456.1"
                     /translation="MKSGFMKLALVALTLTLFMPQVRAENAAPAAPVKAPGKSAVQTA
                     VKIGYFNLNLVKISYPEAAGSEALRAQAECQLRKDVEDANKKISKAQEEKKAPEELQK
                     MAKEVQAEINAKQQALAQLVQSAQSQATEKLYTAVNMVAKEKGLDVIVDGAGVFAGGK
                     MVLENGQDVTLDIITKLSPASALLRPTSASK"
BASE COUNT          630 a          648 c          793 g          673 t
ORIGIN      
        1 aagtcttgat ccagaccggc atgatcgctc cgggtgtggt ggatgagagt ctgcgtttgc
       61 aggaaatggt ttccagcggc gaactgagcg gcttgcaagc tgccgacatt ttgcgtcagg
      121 ctaatgctcg caaagtcggt atagatgtaa tcatggaaga gagagcttct cgcaaagaag
      181 aggttgagaa gattaactcg gtcctggaag tgatcttgca atccggcgcg ctcagcggcg
      241 agaacttcaa ccgggcccag tcgctggctc ggcagttgaa tgtttctatc gccgaggtca
      301 ttctcactaa agagatggta gaaaaacgcc tcattatggc tgcatatcag gggcaatccc
      361 tggtcaatga cggtattatc aatggtgcgc aatgcgtgct ggccatgaaa atgtgcgtca
      421 agaccggcaa ggagttccac gaggttctca aagaattgcc ttccgatacg gctgaatcca
      481 gtcctcctgc cgacgattcc gccggcggag ataagaagag tggttggttg ggaagtctct
      541 ggtccaaagt caccaagaag gactgaagct tactgtgctg accttttcac tggatgagct
      601 tactagcctg gctgcaagcc cgcttgtcgg cacccttccg gatgacggcg gcctgtccgg
      661 cgcctttcgg ggaagcgtat gcaccgatag ccggcaaatc gaaagcggtg atgtttatct
      721 ggctctaaaa ggagagcgtt ttgacgggca ccgcttcgtc aagaatgccc tggatggtgg
      781 tgcggccctt gccgtggtgg aagcctcccg ggtggagtct ttgctaaagg tctgtcaggg
      841 accgcttctg cctgtgccgg atacactcaa gtactatcag ggtctggctc gcctggccag
      901 gcgcaggcag aagccattcg tgatcggcat caccggctct tccggcaaaa ccaccaccaa
      961 ggaaatgtgt tttgctgtct tttctgccag tgccggtaag gttgttcaca agtcggcagc
     1021 caatgaaaat aacgagatcg gcgtgcccaa gaccattctc tctatgcctg aaaataccga
     1081 ctttctgatt gtggaaatgg gcatgcgtgg gctcggacag attgatgaat tggcctctac
     1141 tgccgaaccg aacctcggta tcatcacagg tgcaggttcc acgcatattg aactgttagg
     1201 tagtcgggaa aatattgcaa aagcaaagtg cgaactattt gcccatattg atccatcctc
     1261 tgggtttgct atttgcggcg acttctcgcc gctcctgaag gaggcggctg ctggggtgtt
     1321 taccggtcgg gtttgcgact ttgacggcga agatctgcag gttcgaagaa taaccgcgac
     1381 tggtaccagt ttcagccttg gaagtgagga gtttttcgta caggctcacg gtgattttct
     1441 tttgcgtgat gcctggtgcg cagtcaaagc cggtctgtgt gccggtctta cccctgagac
     1501 catcaggcag ggtctctcga actggcgtgc cgtcgaaggc cgcggtaatg tgattgctac
     1561 cggtagcggt ctcaccttaa tcgacgaatc ttataacgcc aatccggatt cggtgcgctg
     1621 tgcagtcgag tctgtacttg cttgcgaagc ctatcaaggc aagcgcaaaa ttattgttct
     1681 tggtaagatg gccgaacttg gtgatttcac tgaggcgctg caccgggagc ttggttcctg
     1741 gctgaaaacc aaggctattg attgcctggt tacagtgggg gcagaggctg gaatgattgc
     1801 ctctgaagcc ggtggtgccg cctttgaggt catgccctgt gcagatcagg aggaggccca
     1861 taggcggttg cgtgagacta tttctcaagg cgactgcatt atggtcaaag gttcccacag
     1921 tacgaatcta gaagcgcttg tcagacgctt agagcaagtg gtcggtgatt gacggttcag
     1981 caggagattt ttaagtgttg atgaaatccg gttttatgaa gctagcacta gttgccctta
     2041 ccttaaccct cttcatgccg caagttcggg ctgaaaacgc cgctccggct gcgcctgtta
     2101 aggctccggg caagtctgcc gtgcagactg cggtcaaaat tggttacttc aatctcaatc
     2161 tggtcaagat ttcctatccg gaagctgccg gttccgaggc tctgcgggct caggcggaat
     2221 gtcagcttcg caaagatgta gaagacgcca ataagaaaat ttctaaagct caggaagaga
     2281 aaaaggcgcc tgaggaattg cagaaaatgg caaaggaagt gcaggctgag attaatgcca
     2341 agcagcaggc tcttgcccaa ttggtgcagt ctgctcaaag tcaggcaaca gagaaactct
     2401 acacggccgt gaatatggtg gctaaagaga aaggtctcga tgtaattgtt gacggcgccg
     2461 gggtttttgc cggagggaaa atggtactgg agaatggtca ggatgtaact ctggacatta
     2521 ttaccaaact ttctcccgct tccgcgctgc tccgaccgac atcggcaagt aagtaggtta
     2581 cttgagcaag tctttataag caaagccgaa gaaagtaacg ctgtcggttt cctttagttt
     2641 ttcttcctgc cggcttgcca aaaaagcaag agccgattta actgctgtgt cgtccggttt
     2701 gttaagcagg gcccattcaa ggtaggtttg cgctttggca tggt
//
LOCUS       DAOQNZ010000452         2742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20504_length_2742_cov_3.440268, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000452 DAOQNZ010000000
VERSION     DAOQNZ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20504_length_2742_cov_3.440268"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKW73_08965"
     CDS             complement(<1..368)
                     /locus_tag="PKW73_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73457.1"
                     /translation="MSTFKITTLPSREEVNEAHWRYRLEKELALSGLQTIVGVVAFSL
                     ILFIPAGGMTASFLQGLHLPTIVCITIGFFVGGTVLVGPTLLARSFLTAEREDTLFDS
                     NEIYSSPITYEIQENGIHVRA"
     gene            515..910
                     /locus_tag="PKW73_08970"
     CDS             515..910
                     /locus_tag="PKW73_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73458.1"
                     /translation="MSEENTADMSNQAPAPVPRLGFADNVKEAFKHINRSVKAQRGRI
                     RSADYLSIFLVPVTCVALMAASFCASWSRLIVSLIALCSVLFYVLARIGIMRTLNERQ
                     AALIWHILMATFLMGITFAFVFLEILNSL"
     gene            complement(923..>2742)
                     /locus_tag="PKW73_08975"
     CDS             complement(923..>2742)
                     /locus_tag="PKW73_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA73459.1"
                     /translation="LLLFAGYLSAPVHGKTLRLAQSNNSRSGSVANSSNSSSPTKLPD
                     VVPTMSPVPAGEKAPGMSTVVEGEMPKLRGISNENESRRATIDDLSREVSNSTGSIFV
                     DGENVLVKPPLLKSLITLTQTTNPYQLDAASSQEISLRGVLQQSLMQNLPIKVSQAEA
                     QKEKWIYYGALSGFLPSLTNTLSFQGIKGNYVSPAGLAIPIANPFFSTAAGFNQYLFK
                     GGGILYTALQDKHNYKAARAHLKGTVNDILLDTADDYYNLVRSDVLLQIRIKAVEVSR
                     ALLMVNQDLFDNGVNTQLDVLQAKYQLSADRQHLIKQQVERRKHAVKLATVLNADTGV
                     DYSVGARMVSKHRLVDANLKPGDLLKIAIDRRPELKKYDELRMAAKDAVKVARSALLP
                     AVAVTGTVIGTGSRAVNSPLSGASNQQTTLSSNGLSAGAVSSSAGLPLAAPSSNSTGS
                     PHYTTRSLFTIGVGVNWNLGGLGMQEVAQVRASQYVARQAQLEFQRELERITKQVRDA
                     YLSSMSAENLIIETTDAVKYAQEGLRLAELRFQEGVGTYLDVINAQHDYTNSLIDKAN
                     ALIDFNLAQVHLVHAIGLPTVDSLTAAKPLKDAPPKATK"
BASE COUNT          615 a          714 c          733 g          680 t
ORIGIN      
        1 gctctcacat gtattccgtt ttcctgtatt tcataagtaa ttggcgaact atagatttca
       61 ttagagtcga agagggtgtc ttctcgttca gcggtcagaa aggatctggc tagcaaagtc
      121 ggacccacca gcactgttcc tccgacgaaa aaccctatgg ttatacagac gatggtgggc
      181 aaatgtagtc cttgcaagaa ggaagccgtc atgccgcctg ccggaatgaa tagtattaga
      241 gagaaggcaa caacccccac aattgtctgg agtccggaga gtgccagttc tttctccaga
      301 cgataacgcc agtgagcctc atttacctct tctctggaag gtagcgtggt gattttgaat
      361 gtgctcaatt tgaacctcaa tacctttatc tcattgcctc cattttaggt catgctccgg
      421 cggctctgat ttatcagccg gtgggttaac ctggcaatcg cccggggtaa gatagatctg
      481 ccggggcgaa ccgcctcggg cagggagaga actcatgagt gaagaaaata cggccgacat
      541 gagcaatcag gcgccagcgc ctgttcccag gcttggcttt gccgacaacg tcaaagaagc
      601 attcaagcac attaataggt cggttaaggc ccagcgcggc agaatccgca gtgccgatta
      661 tctttccata ttcctggtac cggtaacctg tgtcgccctc atggccgcct cgttttgcgc
      721 cagttggtca aggctgattg tctctctcat cgccctctgc tcggtactct tttatgtgct
      781 ggctcgcatc ggtatcatgc gcaccctcaa cgaaagacag gctgccttga tctggcatat
      841 tctcatggcc accttcttga tgggcataac ctttgccttt gtctttctgg aaattctcaa
      901 ctcactctag tccgagttac tactattttg tagcttttgg cggagcatcc ttgagaggct
      961 tagcagccgt gagagaatcc accgtaggca ggcctatggc atgcaccaga tgtacctgag
     1021 ccaggttgaa atctatgagg gcattggctt tgtctatcaa tgaattggtg tagtcgtgct
     1081 gggcgttgat cacatccaga taggtgccta ccccttcttg aaagcgaagt tccgccagac
     1141 gcaagccttc ctgggcgtat ttgacggcat cggtggtttc tataatgaga ttctccgcgc
     1201 tcatactcga caggtaagca tcccgaacct gcttggtaat gcgctccagt tctctctgaa
     1261 attccaattg cgcctgcctg gccacatact gcgaagctcg cacctgggcc acttcctgca
     1321 tgccaagacc gccgaggttc cagtttacgc ccacaccgat ggtgaaaagt gagcgggtgg
     1381 tgtagtgggg cgagccggta ctgttcgaac tgggagcagc caggggcaag ccggcgctcg
     1441 atgatacggc accggcactg agaccgttgg aactgagggt ggtctgctga ttgctggcgc
     1501 cggaaagggg tgaattgaca gcccgggaac cggttccaat caccgtaccg gtcactgcca
     1561 cagccggcag aagggccgaa cgagccactt tcactgcgtc tttggcagcc attctcagct
     1621 cgtcatattt tttcagctcc ggtcttctat cgatagctat tttgagcagg tccccgggtt
     1681 tcagattggc gtccaccagg cggtgcttcg aaaccatgcg agcaccaacg gaataatcga
     1741 ctccggtatc ggcattcaat accgtagcca gttttaccgc atgtttgcgg cgctccacct
     1801 gctgtttgat tagatgctgg cggtctgcgg aaagctgata tttagcttgc aatacatcaa
     1861 gctgtgtgtt gacgccgttg tcgaaaagat cctggttgac cataagcaac gcccttgaaa
     1921 cctctacggc cttgatgcga atctgcaaaa gcacgtccga acgcaccagg ttgtagtaat
     1981 catcggcggt atcaagcaaa atatcgttga cggtaccttt cagatgggca cgtgccgctt
     2041 tgtaattgtg cttatcttgc agagccgtat agagtatgcc gccgcccttg aagagatact
     2101 gattgaagcc ggccgccgta ctgaaaaacg gattggcgat gggaatggcc aatccggcag
     2161 gtgaaacata attacctttg ataccctgga agctcaaggt attggtcagc gaagggagaa
     2221 aaccgctgag ggcaccgtag tagatccact tttctttctg cgcctcagcc tgagagacct
     2281 tgataggcag gttctgcatg agcgactgct gcaacacgcc acgcagactt atctcctgcg
     2341 aggaagccgc atccagttga tagggattgg tggtttgcgt gagagtgata agcgatttca
     2401 aaaggggagg tttgaccagc acgttctcgc cgtccacgaa aatactgccg gtggaattgc
     2461 tcacttcccg ggagaggtcg tcgatggtgg ctcgacggct ctcgttttca ttgcttattc
     2521 cacgcaattt cggcatttcg ccttccacaa ctgtggacat gccgggcgct ttctctcccg
     2581 ctggcaccgg cgacatagtc ggcaccacgt caggcaattt agtcggcgag ctagagttgg
     2641 aagaattagc gacggagcct gatcgactgt tattgctttg agccaatctc aaggtctttc
     2701 catgcactgg cgccgagaga taaccggcaa agagaagcag gc
//
LOCUS       DAOQNZ010000453         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20553_length_2739_cov_3.494784, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000453 DAOQNZ010000000
VERSION     DAOQNZ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20553_length_2739_cov_3.494784"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PKW73_08980"
     CDS             <1..806
                     /locus_tag="PKW73_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNA73460.1"
                     /translation="IAGPRVLEHMVDVVLQFEGDSLRQLRLLRAVKNRFGSTSELAIF
                     AMMEEGLKEVENPSALFLGDRLSKVGLKQAPSGTAVIAGGEGSRSLLLEVQALTVATP
                     NPSPRRVVNGWDYNRLLQLLAVLEKRAQLSLCRLDVYVNIVGGMDFSDPAGDLGIALA
                     VATSFLDRSIDPGLVAVGEVGLTGEIRPVINLAQRLKEAHRLGFTKALVPRVNLPLSG
                     VPKGLEVIGVESISEALNYAIPSIDKQTLKDKASQEQVLTRELELNKLG"
     gene            834..2711
                     /gene="uvrC"
                     /locus_tag="PKW73_08985"
     CDS             834..2711
                     /gene="uvrC"
                     /locus_tag="PKW73_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNA73461.1"
                     /translation="MRDYQPQELIVRQLSTLPDSPGCYLFKDQDGEILYIGKAINLKS
                     RVRSYFVEASWRERPKLAVMIPKVVSIETILVNSEKEALLLEANLIREKKPRYNVALK
                     DDRRYPWLAITYDVAYPRLVMIRDPAQYKKNNPHSKVFGPYVEAGAMWETVKTLRKVF
                     PMRQRHKPLFKDRPCMNYHIGLCLAPCQKLVQEETYNKMVKQVEMFLSGRQTEVMDEM
                     TREMEALSEALEFEEAALIRDRLYALSTVIEKQQVFFQNRRVCQDIIAQAHTEKLLAL
                     CLMKVREGKLIASETVTLPLLDKTGWDEAFQAYICQYYTACEDIALPAEVLLAHEIED
                     IELLRDFLSQKAGVNVKVLVPQRGGKTDLIEMAQKNAQQALTRVLTEDSENDARVERI
                     LTALKEGLGIDRLPKRIECFDISNIQGTDNVASMVVFEGAKPKKSDYRMFKIKSVEGT
                     PNDFASMKEAVGRRYARILAESKPFPDLIIIDGGKGQLGAAFEALTELGLTGPGFKTE
                     KFAICGLAKRQEEVYFPGNSTPVFLPRRSEALLLLQHVRDEAHRFAVTYHRKLRAKRV
                     VSSKLDSLKGLGAKRRKLLIDHFGSFEKKKKNQKRTRASSVTYLLTYLLLFSFGSSSS
                     F"
BASE COUNT          642 a          676 c          760 g          661 t
ORIGIN      
        1 ccatcgccgg tccgcgcgta cttgaacaca tggtggatgt ggtcctgcag tttgaagggg
       61 acagtttgag gcagcttagg ctcttgcggg ccgtcaagaa ccgtttcggc tccacctcgg
      121 aactggctat tttcgccatg atggaagagg ggctgaaaga ggtggagaat cccagcgcgc
      181 tcttcctggg agacaggctt tccaaagtcg gcctcaagca agcgccctcc ggcaccgccg
      241 tgattgccgg cggcgagggt agtcgcagtc tgcttctgga agtgcaggcg ctcacggtcg
      301 ccacccccaa tccctcgccc aggcgagtag tgaatggttg ggattacaac cgtcttttgc
      361 aacttctggc cgtactggag aaaagagccc agctttcact gtgccgtctg gatgtctatg
      421 tgaatattgt aggcggcatg gattttagcg atccagccgg cgatctcggt attgctctgg
      481 cggttgccac atcatttctt gatcgctcca tcgatccagg cctggtggca gtgggagaag
      541 tgggtctgac cggagaaatt cgaccggtca taaatctggc ccaaagactg aaagaggctc
      601 atcgacttgg ttttaccaag gcccttgtgc cgcgggtcaa cctgcccctg agcggtgttc
      661 caaaaggtct ggaagtaata ggcgtggagt ctatttccga ggccctcaat tatgcaatac
      721 cgagtattga caaacagacc ctcaaagata aagcttccca ggagcaagtc ttgacgcggg
      781 agttggagtt gaacaagcta ggttagtttt tttcggcagc aagatttagg caaatgcgcg
      841 actatcaacc tcaagaactc attgtcagac agctttccac cctgcccgat agtccaggct
      901 gctatctctt caaagaccag gatggcgaga ttctctatat aggcaaggcg atcaacctca
      961 agagccgggt gcgttcttac ttcgtggaag ccagctggcg agaacgtccc aaattggcgg
     1021 tgatgatacc gaaagtggta tcaattgaaa ccattctggt caactccgaa aaagaagcgc
     1081 ttttgctaga ggctaattta attcgagaga agaagccccg ctacaatgtg gccctcaagg
     1141 atgaccggcg ttatccctgg ttggccatca cctatgacgt ggcttatccg cgcctggtaa
     1201 tgatccgtga tccggctcag tacaagaaaa acaatcccca ctcgaaagtc ttcggaccct
     1261 atgtggaagc cggggccatg tgggagactg tgaagacctt gcgcaaggtc tttccgatgc
     1321 gccagaggca taagccgctt ttcaaagatc gtccctgtat gaattaccat atcgggctct
     1381 gtctggcgcc ctgtcagaag ctggtgcagg aggagaccta caacaagatg gtcaagcagg
     1441 tggagatgtt tctctccggc cgccagactg aagtaatgga tgaaatgacg cgggagatgg
     1501 aagcactctc ggaggcgctt gagtttgaag aggctgcctt gataagagat aggctctatg
     1561 ctctttccac ggtgatagag aagcagcagg tattctttca aaatcgacgc gtctgtcagg
     1621 atattattgc ccaggctcat acggagaagc ttttggctct ctgtttgatg aaagtgcggg
     1681 aaggtaagct gattgcatcg gagacggtga ccctgccctt gcttgataag accggctggg
     1741 acgaagcctt tcaggcttat atttgccagt attacacggc ttgcgaagac attgccctgc
     1801 cggcggaagt gctactggcc catgagattg aagatattga gcttttgcgc gacttcctca
     1861 gtcagaaggc cggtgtcaat gtgaaggtac tggtgccgca gcgcggcggc aagacggacc
     1921 tgattgagat ggctcaaaaa aatgcgcagc aagccctcac ccgtgttctc actgaagact
     1981 cggaaaatga tgccagggtg gaacggattt tgaccgctct taaggaaggg cttggtatag
     2041 accggctgcc caagcgcatt gagtgcttcg acatttccaa cattcagggc accgataatg
     2101 tggctagcat ggtggttttc gaaggggcca agccgaaaaa atccgactat cgtatgttca
     2161 aaataaagtc ggtggaagga acccccaatg actttgcctc catgaaggaa gctgtcggca
     2221 ggcgctatgc aaggattctt gccgagtcta agcccttccc cgatttgatc attatcgacg
     2281 gcggtaaggg gcaattgggt gccgccttcg aggcactgac cgaactgggg ttgaccggac
     2341 ccggcttcaa gactgagaaa ttcgccattt gtggtctggc caagcgacag gaagaagtct
     2401 atttccctgg aaattccacc cctgtgtttt tgcccaggcg ttcggaagcc ttgcttttgc
     2461 tccagcatgt acgggatgag gcgcaccgtt ttgccgttac ttatcaccga aaactgcggg
     2521 ccaagcgggt tgtctcttcc aaactggatt cgctaaaagg gcttggtgcc aagcggcgta
     2581 aacttctcat tgatcacttc ggctccttcg aaaagaagaa gaagaaccaa aagagaacaa
     2641 gagctagctc agttacttac ttacttactt acttactctt gttctctttt ggttcttctt
     2701 cttctttttg attggcggcc ggtgtcttct cttttgctg
//
LOCUS       DAOQNZ010000454         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20610_length_2734_cov_4.623367, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000454 DAOQNZ010000000
VERSION     DAOQNZ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20610_length_2734_cov_4.623367"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKW73_08990"
     CDS             complement(<1..237)
                     /locus_tag="PKW73_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73462.1"
                     /translation="MPENTEACPSCGRPTPVGTRCVYCATVTDLDLKSLQLDYLKQSD
                     SKAPEEPIQNTVVVVVQKTGQRYSLKTSKVKIGRD"
     gene            complement(503..847)
                     /locus_tag="PKW73_08995"
     CDS             complement(503..847)
                     /locus_tag="PKW73_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HNA73463.1"
                     /translation="MVFQDKDINCRDCNKTFVFSAGEQEFFHTKGLVNEPKRCPNCRI
                     LMRVQRNGEDPGRTAEVACAECGSPTRVPFQPKGYKPVYCTYCFRTRKGGAEDDAAKE
                     PETETISQTASA"
     gene            complement(1061..2071)
                     /gene="tsaD"
                     /locus_tag="PKW73_09000"
     CDS             complement(1061..2071)
                     /gene="tsaD"
                     /locus_tag="PKW73_09000"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNA73464.1"
                     /translation="MLYLGIETSCDETACALVEDGRKLISGRLISQTEVHKAFGGVVP
                     EVAARRHLEVFNGLLNEMFEEACLKLSLPLRGDNENGLLAQIGAVACTNGPGLIGTLL
                     VGLAGAKTLAWALEKPLLAVDHIQAHVCANYIDSDLEPPFVALVVSGGHTQIMHFSSY
                     KEFAVLGQTLDDASGEAYDKVARLLGLGYPGGPAIDKLAATGNPRAYNFPEGRVEGLN
                     FSFSGLKTAVMRTMENHSGVLNTADVAASFQDAVNRVLFKKTVNCARELGLNRIVLAG
                     GVAANSDLRRRFSELSEAGTYRVYRPPLSLCTDNAAMVASAAFFSGVPAPLSIGAYSR
                     GA"
     gene            2413..>2734
                     /locus_tag="PKW73_09005"
     CDS             2413..>2734
                     /locus_tag="PKW73_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73465.1"
                     /translation="MKNHTFKFPRTNAFLAGAFLSVLAAWASVTTASAQSIDGSFDLG
                     GGATGGVTMGANGPTGVLSNGATGDSIQIGGGGPGFALSSGPASLGDSGTIGRTSFRT
                     GYGNQ"
BASE COUNT          675 a          755 c          702 g          602 t
ORIGIN      
        1 gtcgcgaccg attttgactt tagaagtctt gagcgagtat ctctgcccgg ttttctgaac
       61 caccaccacc acggtgttct ggatcggctc ttcgggagcc ttggaatcag actgcttgag
      121 atagtcgagc tgcaaactct tgagatccag gtcggtgacg gtcgcgcagt agacacagcg
      181 ggtgcctacc ggagtgggac gtccacaact gggacaggct tctgtatttt ctggcatgcg
      241 actcctaacc taaccacaag cgcttggttt ccatgaagcg cttctgagat acagagctat
      301 tatacgcacc cattacggct acaaaataca aggtttgata gatgggaaac aaattcttta
      361 gagaatatag aaaagaagat tccgaattgg gctaatctca ctttgccccg cgccaaccaa
      421 gtggtaacaa ttggcccaaa atttcctcag gcgtttaatc gcgcaagggt aagcgaggca
      481 gcgcagtctg atcagtatcg agtcaggcgc tggcagtctg agaaattgtt tcggtttccg
      541 gttctttggc tgcatcatct tcagcaccgc ctttgcgggt gcggaagcag taagtgcagt
      601 aaaccggctt atagcccttg ggctggaatg gcacccgggt gggcgagccg cattcggcgc
      661 aagctacttc ggcggtgcga ccgggatctt ctccgtttcg ctgcacacgc attaagattc
      721 gacaattggg acaacgcttg ggctcattca ccaagccttt tgtgtggaaa aactcttgct
      781 ccccggcaga gaacacgaaa gtcttattac agtcccgaca atttatgtcc ttatcctgga
      841 aaaccatggc atgccacctg tgatataggg gtttccttag tcaagcgtta aacaggcaga
      901 agcttagtaa aaggctgaca agagttgcga cgataatgcc atggtatcac gaacagattg
      961 gcaccgcata tcacaaaccc acgccctggg gttagcacca tagaaatatg gcaatctcca
     1021 tagcatgatt gcccctgaga agcagtctag aagccgaaac ctaggcgccc cttgagtatg
     1081 caccaataga gagaggagcc ggaacaccgc taaagaatgc ggcactggct accattgccg
     1141 cattgtccgt acaaagggag aggggtggtc gataaactcg ataagtaccg gcctcggaca
     1201 gttcactgaa gcggcgcctc aaatcgctat tggcggcgac cccgcccgcc agaactatgc
     1261 gattcagccc cagttctctg gcacagttca ctgttttctt gaatagcaca cgattgaccg
     1321 catcctggaa ggaagcagcc acatcggctg tattgagcac accgctatgg ttttccatag
     1381 tgcgcattac cgccgtcttc aacccactaa aggaaaagtt gagcccttcc actcggcctt
     1441 ccgggaaatt ataggccctg gggtttcccg tagcagccaa cttgtcaatg gccggtcccc
     1501 ccggataacc cagccccaac aagcgggcca ctttgtcata ggcctccccg gaggcatcgt
     1561 ctagggtctg accgagaacg gcgaactctt tataagagct gaagtgcata atctgcgtat
     1621 gtccgccgct gaccactaag gctacaaaag gcggttccag gtcggaatct atgtaattgg
     1681 cgcagacatg ggcctgaatg tgatcgacgg caagcaaagg cttttccaga gcccaggcca
     1741 gggttttggc acctgccaga cccaccagga gcgtaccaat caaacccggg ccgttggtac
     1801 aggcgacggc accaatttgt gccaataatc cattttcatt atcgccccgc agaggcaaag
     1861 agagcttgag gcaagcttct tcgaacatct cgttcagaag tccattaaaa acttctagat
     1921 gtcgcctggc tgctacttcc ggcaccacac caccgaaggc tttatgcacc tcggtctgcg
     1981 aaatcaggcg tcccgagatg agtttgcggc cgtcctccac cagggcacag gcggtttcat
     2041 cgcagctagt ctcaattcca aggtaaagca aggataactc cccggtcgca cgatcttgac
     2101 tacactagca cagacagccc atccaagccg gtaatttggg gttccggatt taaccttgca
     2161 aacaaaagaa cctattgaca atgaaggggt tgaagcttac tatctttcca attagcgccc
     2221 gattcaggtg cccaatttcc gggcacaatc tataaagggt gtatccaagg aaaatcccaa
     2281 cgggaattat ccccgggctc caatctatcg ttctttggat gctctctgat caggtgctaa
     2341 gggtacggaa gcagttccgt ccttcttact aaacaatcgg cttacagatt caggtcatca
     2401 ggtggccagc caatgaaaaa ccatactttc aagtttccaa gaaccaacgc ctttctggca
     2461 ggtgcctttc tatccgtcct tgcggcatgg gcctcagtga cgacagcctc ggcccaatca
     2521 atagacggct cttttgacct gggcggcggc gccacgggcg gggtgaccat gggagccaac
     2581 ggtcccaccg gtgtactttc aaacggagcg accggcgatt cgattcagat aggcggcggc
     2641 ggaccaggct tcgccctctc cagcggaccg gcttccctgg gagacagcgg cacgatagga
     2701 cgcaccagct tccgcaccgg atacggcaac cagg
//
LOCUS       DAOQNZ010000455         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20768_length_2722_cov_3.989876, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000455 DAOQNZ010000000
VERSION     DAOQNZ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20768_length_2722_cov_3.989876"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..602)
                     /locus_tag="PKW73_09010"
     CDS             complement(69..602)
                     /locus_tag="PKW73_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA73466.1"
                     /translation="MPSLQFQKPSQITMKSCPHCTAMLKVTGIGGKDRLACELCEFVH
                     WDNPKPVTATLVTMDSELVLVKRKFEPFVDDWCLPGGFIEASEDPEESAAREVFEETG
                     LEVRIEKLLGASAPGHGINVIILFYQGTPVGGRLEAGDDASAVGTFKPDSMPKNIAFP
                     LHRKMISQFFQSVKSEA"
     gene            1598..>2722
                     /locus_tag="PKW73_09015"
     CDS             1598..>2722
                     /locus_tag="PKW73_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73467.1"
                     /translation="MSNQKTGVAQTLLNLVLFIPTVLYMAVRESGKWICRRAYSGGFV
                     RAIFVGLAGFGVACVTSISAADYFSGQLGWSPSAWFVSSAVVFILTMTHLWPAIYWGL
                     VRHVWNLGEWILKKVKLFAKEVFKPLTAIVVNAMRQAPGSNYLWELVEGKNGKKWGIR
                     LLQVLLFLGAALLSALAGYVTFQALAGLVPVPMLAALHLEEIVAGVVAFTAFYFSISS
                     LVYLLDEGDAPATAMVYSGLAAYGLTHTSLLAGASLPVVLGACAFTFLAGVTYILPAF
                     IAVLQGGLVERLLKAWGELLDSVYAREDNLEFRRFYHHVMNIVISAAVGCVLYANVGL
                     VNLPPLAGLVLSVLVGVFCYAEGSRDILNRSGGNAMIGILT"
BASE COUNT          511 a          763 c          760 g          688 t
ORIGIN      
        1 cgggaaatgc tgccggctcg aagctgacca gcttccaagc aagagccgcc gatttttcac
       61 tgttagtttc aggcttcgct tttgacggac tggaagaact gactgatcat ttttcggtgc
      121 aggggaaaag cgatattctt aggcatactg tcaggtttga aggtgccgac tgcactggcg
      181 tcgtcgcccg cttccagtct gccgcccacg ggagtgccct ggtagaaaag gataatgacg
      241 ttgataccgt gaccgggtgc actggcaccc agaagtttct caatgcgcac ttccaaaccg
      301 gtttcttcga atacttctcg ggctgccgat tcttccggat cttcactggc ttcgataaag
      361 ccgccgggca ggcaccaatc gtctacgaaa ggctcaaatt tgcgtttgac cagcaccagc
      421 tccgagtcca tggtgaccag ggtggccgtg accggtttgg gattgtccca gtgtacgaat
      481 tcacaaagct cgcaggcaag gcggtctttg cccccgatac cggtgacttt caacatggcc
      541 gtgcagtggg ggcaactctt catggttatc tgggatggtt tctggaactg aagggatggc
      601 aagtgtcccg tgctgctgcc cgaaagcggc ttaagcactt gtcattactc ggattgcatc
      661 cctagaattt tatcagcaat ccgctctttg caatgggaaa ttgggagcca gggctgaatc
      721 tcgtagaata tgcattggta gaaagtctga taaaagttga atattctatg cgtatgctat
      781 ctgctcctgg taacatgcaa aagaccgttt accccacttg gtggtgggga atataagaca
      841 ctggaatccc gtcctcgatc gagtcgaggc tagctaaggt agaaaacaat gagttcggag
      901 tcccttggat actttgctct cgggcagtcg aaatcctcaa gtcagtctgg agaacgtgcc
      961 attgagcgcg accgccagac cgaagcaacg agaaactgca agaacggtgt ttgtgagctt
     1021 tctacctgga agccgagcaa gccgtcagta acccaagacc ctgccgatgg ggttcgtcgc
     1081 aagaacatct aagtgaagct tgcttcaatt tttacttaaa agccgcttat gaatgcggcg
     1141 cggtttatcc gtttcaagtg cttgaaacct gcctttcgaa ggcaggtttt tttgctctcg
     1201 cgagccgcgc tattgtggcg caagttcttg ggagcattac ctttctgtta ttaaggctta
     1261 gaactcgttt ccgaatgcct gaatgactca gaagtaattt atagaagctt ttgtagggct
     1321 cgtcgcttta tctaggctgc tgacatctac ttccacaaat ttcgagatcc gatttccgag
     1381 caaccgctgc aattatcccc cctgcactgc catttccagc ttactccagt ggtttccacc
     1441 ctggtgctga aaacgtctcg cgttctggta gatgacgtag agatggtggc ggatagtttg
     1501 ggtggggctg gctttgctgc ggcccaagac gctcgggcgg atttctaacc cctcctcgca
     1561 acgaggagga ttgcttcccg taaggagaat gcaccaaatg tccaaccaaa agactggagt
     1621 ggctcaaacc ctcctcaacc tcgtgctgtt cattcccact gtcctctaca tggctgtgcg
     1681 tgagagtggc aagtggatct gccgtcgcgc ctactccggt gggttcgtgc gtgcgatttt
     1741 cgtcggtctg gccggtttcg gggtggcctg tgtcacctcc atttccgctg ccgactattt
     1801 ctccggacag ctgggctgga gtccttccgc ctggttcgtc tccagcgccg tggtcttcat
     1861 cctgaccatg acgcacctct ggccggctat ctactggggt ctggttcgcc atgtctggaa
     1921 tctgggcgaa tggattctga agaaggtgaa gctgttcgcc aaggaagtct tcaagccgct
     1981 cacggccatc gttgtgaacg ccatgcgcca ggctcccggc tccaactacc tgtgggagct
     2041 ggtggagggc aagaacggca agaagtgggg catccgcctt ctgcaggtgt tgctcttcct
     2101 gggcgctgct ctgctctcgg cgctggccgg ctacgtcacc ttccaggccc tggccggact
     2161 ggtgcctgtt cccatgctgg ctgccctcca tctggaggaa atcgtggccg gcgtggtggc
     2221 tttcaccgcc ttctacttct ccatctccag cctggtctac ctcctcgacg agggtgatgc
     2281 tccggctacg gccatggtct acagcggcct ggctgcctac ggtctgacgc acacttcgct
     2341 cctggctggt gcctccctgc ctgtggtgct gggcgcttgc gccttcacct tcctggccgg
     2401 cgtcacctac atcctgccgg ctttcatcgc cgttctgcag ggtgggctgg tggagcggct
     2461 gctcaaggcc tggggtgagc ttctggactc ggtctatgcc cgcgaggaca atctcgagtt
     2521 ccgtcgtttc taccaccacg tcatgaacat cgtcatctcc gctgccgtcg gctgcgtcct
     2581 ctatgccaac gtggggctgg tcaacctgcc tcccctggct ggtctggtgc tgtcggtgct
     2641 ggtgggtgtc ttctgctacg ccgaaggcag ccgcgacatt ctcaaccgca gcggcggcaa
     2701 tgccatgatc ggcattctca cc
//
LOCUS       DAOQNZ010000456         2719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20811_length_2719_cov_3.368619, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000456 DAOQNZ010000000
VERSION     DAOQNZ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20811_length_2719_cov_3.368619"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..636)
                     /locus_tag="PKW73_09020"
     CDS             complement(13..636)
                     /locus_tag="PKW73_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73468.1"
                     /translation="MKLFTLTNKGTLFAGATLPLQRTNEGQYYIRGQLKAGPAVAIDL
                     LLSPDAQIIGADFPRGAELIIRRASVEVNSEGHYRLTSERPLTVTQNADGHFLQVLPD
                     APESRPDSVLVWIPDLKEEIEVNQGRVVGGVSKIIENSHGSIQRIGSGGLVRMKPYSS
                     FTLATYHNLSTQENKSRQLSSVAVRYDGNGHFSVAGQFVPDYNLKWS"
     gene            complement(777..1193)
                     /locus_tag="PKW73_09025"
     CDS             complement(777..1193)
                     /locus_tag="PKW73_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73469.1"
                     /translation="MPLLNLLKSYAYVFTATFLLILVSNFFALSAFAIAPWTHLLAMA
                     AFISALAWLIYLALILSVSTVLQLKEPGFALATVFGVVSGATALKITSILFAGAIGLL
                     SLTALAAVALLNTLLVWLLAFATGTLRKELSFLPKR"
     gene            complement(1221..1700)
                     /locus_tag="PKW73_09030"
     CDS             complement(1221..1700)
                     /locus_tag="PKW73_09030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA73470.1"
                     /translation="MNTLIRFRIRKAAMADKAAILKINNAVLDCPFEEDWLDRILAES
                     PDKVLVAVREETVLGFLTFICREEGVYITYMGVDSEIQRCGIGKALLKKVIDALPDHG
                     KNQIRLHVKASNLAAISFYEAAGFRPAAQINNYYGKGKPAFLMRFDTHSLQSLLNGS"
     gene            complement(1785..2276)
                     /locus_tag="PKW73_09035"
     CDS             complement(1785..2276)
                     /locus_tag="PKW73_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73471.1"
                     /translation="MTFTQNTQLKPLSYVLAAALASAMLWTGWFIVVSLYLAPLTLLG
                     KADTLPTNLPHWLALLAGAAVFYRIYQTQSRFLLKEALLSSLALSFILILAFFAASLL
                     ALGAVSAAYLISATTNSATVAVIVCSTAWCLSSIYYWVTVIPWLRQLVAPLTEEGKLR
                     LNF"
     gene            complement(2305..>2719)
                     /locus_tag="PKW73_09040"
     CDS             complement(2305..>2719)
                     /locus_tag="PKW73_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNA73472.1"
                     /translation="PGAEYTQMGWEVHPQALRRLLNRIRKDYPEVPPLYVTENGAAYE
                     DLVTQAGRINDTKRLEYLRRHIEQVALAIEDGVDVRGYFAWSLLDNFEWAEGYDRRFG
                     IVHVDFATQERRIKDSGLWYAETARSNKIVLPRNL"
BASE COUNT          631 a          648 c          805 g          635 t
ORIGIN      
        1 ctcctgccag cactaactcc acttgaggtt gtagtccggt acgaactgtc cagccaccga
       61 gaagtgaccg ttgccgtcgt agcgcacagc caccgaactc agttgtctgg atttgttttc
      121 ctgcgtcgat agattgtggt aggtggctag agtgaaggaa ctatatggct tcattcgcac
      181 tagaccgcct gagccgatgc gctggatgct gccatggctg ttctcgataa tcttggagac
      241 tccgcctact acccgtccct ggtttacttc gatttcctct ttaagatccg gtatccagac
      301 cagaacagag tccggtctac tttccggcgc atcaggcagt acttgcaaga agtgtccgtc
      361 ggcattttgc gtcacggtca gaggtctttc gctggtaagg cgatagtgac cttcgctatt
      421 cacttccacg gaagctcgtc tgatgattag ctcggccccg cgagggaagt cggcgccgat
      481 tatctgcgca tcagggctga gaagcaggtc gattgccacg gcaggaccgg ctttgagctg
      541 tccgcgaata tagtattgac cttcgttggt tctttgcaga ggcagggtgg caccggcgaa
      601 gagggtgcct ttattggtga gggtgaaaag tttcatgtct tgtgctccat ctgtgacggc
      661 atatagccgc acggttaaac cggaaagcat tccggctaag ggtgaccggc agagacaatc
      721 tgccggcagg ataggttttt ctcaatctca agggcgtgct gcgcttggga aatttgttag
      781 cgcttcggaa ggaagctcag ctcttttctc aatgtgcctg tagcgaaggc cagaagccaa
      841 accagcaagg tattaaggag agcaacggcg gcgagagcgg ttaagctgag cagtccgatt
      901 gctcctgcga aaaggatcga ggttattttc agcgccgttg ctcctgatac cacgccaaag
      961 acagtggcca gagcaaagcc gggctctttc aactgaagta cggtagaaac agacaagatg
     1021 agcgccaggt agataagcca ggccagggcg gaaataaaag cagccatagc taacaggtga
     1081 gtccaggggg caatggcgaa ggccgacagg gcgaagaaat tagagacgag gatgagtagg
     1141 aaggtcgccg taaacacata ggcatacgac ttaagtaagt tcaaaagcgg catggggtct
     1201 cctctttgaa aagttgattc tcaggaccca ttcagcagag actgcagaga atgggtatcg
     1261 aagcgcatca ggaaggcggg cttgcctttt ccgtagtaat tgttgatctg ggcggcaggg
     1321 cgaaagccgg ccgcttcgta gaaagaaatc gctgctaagt ttgaggcttt cacatgcagg
     1381 cgaatttgat tcttaccgtg gtccggcagg gcatcgatga ctttcttgag cagggctttg
     1441 ccaatgccgc agcgttggat ttctgaatct acacccatgt atgtaatata gactccttcc
     1501 tctctgcaga tgaaagtgag gaagccgaga acagtttctt cccgtactgc taccagcacc
     1561 ttatcgggac tttcggctag aatcctgtcc agccagtctt cttcaaaggg gcagtccagt
     1621 acggcattgt taatctttag gatggcagct ttgtctgcca tagcagcttt acgaatgcga
     1681 aaacgaatta atgtgttcat atgccttccc aggtgctaat agcggcaccg gcaaaaaggg
     1741 ttgtggtggg atcttgaact tcaagatcgt ggttgtgttt gttgttagaa gttgagcctc
     1801 aacttccctt cttcagtgag cggtgctacc agttgtcgca gccaggggat gaccgttacc
     1861 cagtagtaga tgctggagag gcaccaggct gtgctgcaga cgatgactgc tacggtggca
     1921 ctattggtgg tggcgctgat taagtaggcg gcgctgacgg caccgagggc gagcaggctg
     1981 gcggcaaaga acgccaggat caagatgaag cttagcgcta agctggagag cagcgcttct
     2041 ttcaggagga agcgtgattg agtttggtag atacggtaaa agaccgctgc gccggcaagc
     2101 aaagccagcc agtgcggcag gtttgtcggc aaggtatcgg ctttgccgag cagggtgagg
     2161 ggggcaaggt aaagggagac aactatgaac cagcctgtcc agagcatggc tgatgctaag
     2221 gcggcagcca gaacgtatga cagtggtttc agttgcgtgt tttgagtaaa cgtcataacg
     2281 gtaacctcgg ttgataaata gaaattacag gtttcgcggc agcacaattt tgttcgaccg
     2341 ggcggtttcg gcgtaccaga gtccgctgtc tttaatgcgc cgttcttggg tggcgaaatc
     2401 tacatgcaca ataccgaagc ggcgatcgta gccttcagcc cattcgaaat tgtcgagcag
     2461 tgaccaggcg aagtagccac gcacatcgac gccgtcctcg atcgcgagag cgacttgttc
     2521 gatgtggcgg cgcaggtatt ccaggcgttt ggtgtcgttt atgcgacctg cctgcgtgac
     2581 tagatcctca tatgcagcgc cgttttccgt cacgtagaga ggcggcactt ccggatagtc
     2641 cttacggatg cgattgagca atcttcgcag ggcctgcgga tgaacctccc agcccatctg
     2701 ggtatactcc gcacctggg
//
LOCUS       DAOQNZ010000457         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20824_length_2718_cov_2.844536, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000457 DAOQNZ010000000
VERSION     DAOQNZ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20824_length_2718_cov_2.844536"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PKW73_09045"
     CDS             <1..686
                     /locus_tag="PKW73_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73473.1"
                     /translation="RAQGKQPAKQVSFDDDDEDEDGDTAGGAGAQDSHATIEIRSDVF
                     QPKKALYEERIVETEEESTTDLASDLAKSVSAVESVYQELDLFSAEETEFASATVEAA
                     ADSEVVSEAAAAPITYEVESPYEAEVDPVTGIYRLKTKTAEDEAESGVDASSAISLGL
                     DQGVPVLDTSEAVGFGESNDYTPNFTLAREESAKESVSELTLPAFSSLEASESDFGSQ
                     SSEGREDNE"
     gene            complement(760..2010)
                     /locus_tag="PKW73_09050"
     CDS             complement(760..2010)
                     /locus_tag="PKW73_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73474.1"
                     /translation="MTRPINPWKVTGYKAGQNVVCKVTKAETGGYAVIIPKDNLPGFI
                     QTTAALRPGEEILAQFVCVHNNRILLSPLFSGNRASGASLPTSSQVNWAEHLDNPEQI
                     DEILQQRQSETQNNYQAPAEQENYEQSYAPPREEGQQAHSDSTIYAPDYSQSTNQAYQ
                     APIASPENTGASFTESQWQAPIEQTPTKRFRLRRAIDLVMPPLDQESLTTMKMCDYDL
                     EWLITDLEGGMRTGCMKCTSEQALSRSAVLLYRGKAVGCIYGCKTSPDARPTEESLTS
                     MLTDLEAPDATVMVYDLPEEVTVAMASLFLGFPVERDETLDPKTQFDYFMTYFTQNGN
                     TACLAITLPSTKSTYLVFVHKGKFGGAFFVEEQIYTQDPADIYQLFVTDAGARVEASL
                     LQPDMIAAGSRFGYSLSMAKQKRQ"
     gene            complement(2529..>2718)
                     /locus_tag="PKW73_09055"
     CDS             complement(2529..>2718)
                     /locus_tag="PKW73_09055"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HNA73475.1"
                     /translation="LVVTSTRSEEVTMQAIRFGIGQGAKIDITKNIKGGPVILSCNEM
                     ELAVGRELAFLIDVSEIA"
BASE COUNT          579 a          656 c          807 g          676 t
ORIGIN      
        1 ccagggctca gggaaagcag ccagccaaac aggtctcctt tgatgacgat gatgaggacg
       61 aggatggcga tactgccggt ggtgcaggag cgcaggacag tcacgccacc atcgaaattc
      121 gcagtgatgt tttccaaccc aaaaaagctc tatatgaaga acggattgta gagactgagg
      181 aggaaagcac cacggatctg gcttccgacc tggctaagag tgtttcagcg gtagagagtg
      241 tctaccagga actggacctc ttcagtgctg aggaaaccga gtttgcttcc gccaccgttg
      301 aggctgccgc tgacagcgaa gtcgtcagcg aagcggcggc tgctcccatc acctacgaag
      361 tggagagccc ctatgaagcg gaagtggatc cggtgaccgg catctacaga ttgaaaacca
      421 aaacggcgga agatgaggct gaaagcggtg ttgatgcaag cagcgccatc agtctgggtc
      481 tcgatcaggg cgtgcctgtg ctcgatacat ctgaggctgt aggcttcggt gagagcaatg
      541 actacactcc caacttcact ctcgccagag aagagtcggc gaaagagtca gtcagtgagt
      601 tgactctgcc agctttctct tctttggaag cttctgagag tgacttcggt agccagtctt
      661 ccgaaggtcg tgaggataac gagtaattcg ttgaattgga agaaggagcg cttgcccggc
      721 aagcgctcct ttttgcttga atgaagagaa aacgaaagct tactggcgct tttgtttagc
      781 catggagagg ctatagccga agcgtgagcc ggccgcaatc atgtccggct gcagtagact
      841 ggcttccacc cttgctcctg catcggtgac gaagagctgg tatatatcgg ctggatcctg
      901 ggtgtagatt tgctcttcca cgaagaaagc gccgccgaat ttgcccttgt gcacgaacac
      961 aagataggta gacttggtgc tcggcagggt gatggccagg caggcggtgt tgccgttctg
     1021 ggtgaaatag gtcatgaagt agtcgaactg agttttggga tccagcgtct catctctttc
     1081 tacagggaaa ccgaggaaga gtgaggccat ggctacggtt acttcttcgg gcaagtcgta
     1141 gaccatgact gtggcgtcgg gcgcttccag gtcggtgagc atgctggtga gcgattcttc
     1201 cgtgggtctg gcgtcgggag aagtcttgca gccgtagatg cagcctactg ccttgccgcg
     1261 atagagcagc acggctgagc gtgaaagggc ctgttccgac gtacacttca tgcagcctgt
     1321 gcgcatgccg ccttccaggt cggttataag ccactccaga tcgtaatcac acattttcat
     1381 ggtggttaac gactcttgat ccaggggcgg catgaccagg tcgatggcgc ggcggaggcg
     1441 gaagcgcttg gtgggagtct gctctatggg tgcctgccac tgagattcgg tgaaggaagc
     1501 gcctgtgttc tcgggggagg caatgggagc ttgataagct tgattggtgc tctgactgta
     1561 gtcaggagcg tagatagttg agtcggaatg ggcttgttgc ccttcttccc gcggcggtgc
     1621 ataagattgc tcgtagtttt cttgctcggc cggggcttga tagttgttct gggtctctga
     1681 ctgccgctgt tgcagaattt cgtctatttg ctcggggttg tcgaggtgct cggcccagtt
     1741 gacctgcgaa cttgtgggca gtgatgcgcc ggaggcccga ttacctgaaa agagagggga
     1801 aaggagaatg cggttgttat gaacgcaaac gaactgagca aggatttcct cgcccggccg
     1861 caaagcggca gtcgtttgaa taaagccagg caaattatcc ttggggatga ttactgcata
     1921 gccgccggtc tctgccttgg tgaccttgca gaccacgttt tgaccagctt tgtaccctgt
     1981 caccttccag ggatttattg gtcttgtcat tctggttccc ttgcgcgcac cctatgcgcc
     2041 tttagtcgat aatactattt tatgcccctg gatactcaat tccaagcgtt ttttagtagt
     2101 tccgatattg ttcctcacat gtttttgttt gtcggccgtt ttgctcctga gaatatccct
     2161 ggtggcggaa cttacgactt acttttcagg cgtgagtttt tcaggagcgt cgcaagacgc
     2221 ccccgagaac ttttgccagt ctgttaaaga atggttgcaa aggtcctcga ttgttcctta
     2281 ccccgatttc aacagaaatc tcactacatt ccttggaatt tgctaatgag ttacatgccg
     2341 cttcggtttg gccggcctgt ggcttacagg tcaggcagct gcccgagcag gaattttcca
     2401 gttccgtcca gttattgcag cgggggcaga gagccttatt gcccaccagt tggttggttt
     2461 gggaaaggct gaggggcagt ttgcaagtgg aacactgcgg tgcttgatct gccatgggac
     2521 cggacatctc aggcgatttc cgagacgtcg atgagaaaag ccagttcccg gcccacagcc
     2581 agttccattt cattgcagct aagaattacc gggccgccct tgatgttctt ggtaatgtca
     2641 atcttggcac cctgaccgat gccgaagcga attgcttgca tggtcacttc ttccgagcgg
     2701 gtggaagtga caaccagg
//
LOCUS       DAOQNZ010000458         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20879_length_2715_cov_2.863910, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000458 DAOQNZ010000000
VERSION     DAOQNZ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20879_length_2715_cov_2.863910"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PKW73_09060"
     CDS             <1..423
                     /locus_tag="PKW73_09060"
                     /inference="COORDINATES: protein motif:HMM:NF017775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HNA73476.1"
                     /translation="AGLTAARHNAHEIACGGESSETEELQKAKNNLNSVIETLLGIAL
                     VNRRIQFFTTPFNLFVQLIPIVIVVPSYFAGEIPFGTITQAAMAFGAVFNGATVLIGQ
                     FNGITHYAAITNRLGSLAEAMERSQEMETEKVETAKAV"
     gene            complement(952..2319)
                     /locus_tag="PKW73_09065"
     CDS             complement(952..2319)
                     /locus_tag="PKW73_09065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA73477.1"
                     /translation="MSNTDQDFGLDVTYIKQAMKDWDVPGLSIAVVKDGQVVFADGFG
                     LLKKGDGRPVNRRSIFTIGSCTKAFTTVAMAILAREKKISFDDPVQKYVPEFSLADAA
                     VGRALTIKDVVTHQSGVEDGAITNLPSRTLQEAMRHVAGLQATRPHRGGFSYNNTLLA
                     VLALVVEKVSGMSWEDFLQSRILRPLGMKDSFGSVKASARRRNRAVPHHMPRNRKRPA
                     IMKVKSLDYLASSAALQSSIDDMANWLKFQLGTIGREILDKSDLDEMHREHLQVEPNA
                     FTLMMHPGSTRHGYGMTWFVRDYGGAHLVQHGGYVDGFTSFCVMGPAQGFGVVVLTNM
                     HNSLLPFALAYRVIDAYLAQPQVDWSAYFLQKRADHRNKVSPHNEENEALQRLEEEEA
                     LKACLASEGGDLETCRAKTRRRKPGKPGRSRKPGQHGKAKKSGRSSKTGKPAKSRKPG
                     KRRKS"
BASE COUNT          570 a          681 c          779 g          685 t
ORIGIN      
        1 gctggtctta cagccgctcg ccataacgcc catgagattg cctgtggtgg tgaatcgtca
       61 gaaacggaag agttgcagaa ggccaaaaac aatctcaaca gtgtcatcga aacgctcttg
      121 ggtattgctc tggtcaatcg ccgcattcag ttctttacca ctcccttcaa tctctttgtg
      181 caactcattc ccatcgtcat cgttgtgcct tcctatttcg ccggtgagat tcctttcggc
      241 actatcaccc aggctgccat ggcttttggt gctgtgttca atggagccac cgtgttgatt
      301 ggacagttca acggtatcac ccactatgct gctatcacca atcgcctagg ctccctggct
      361 gaagccatgg aacgcagtca ggaaatggaa actgagaagg ttgagacagc aaaagcggtt
      421 tgatattgag gggggcgtgt gtttctttgc ggagattatg ggtggctgat gcttaatctg
      481 ccgtcgacgc cagtagagta attcttgttt gacaagactt tgctggcgtg acgcaggcgg
      541 catctatgtt gaaatacagt cagtaaattt gttggaggtg cttggttttg gtgtaaaaaa
      601 tttgacccta aaatactaaa ggtaatagta gaaatggtgg ttttttttac catgttgatg
      661 aacttgactg ggtctccggt gttttcgagg cgcgtgcgaa aaaatgtaga atttcgctcg
      721 aaagtcaaaa aaatcgctga gataacccca ccattgagcg attttcgtaa taatttcgcc
      781 tttgcctccc ttctcagaag gagacagtgt cggttcttct tcagttcgaa aaggaaagag
      841 aggcaccaga gccggtagca ccggtgcctc tcttttggtt aacggctata gctcaacatt
      901 gagcggtagc ttatcggaat ggatggggac ggattggagc agaccggtgt ttcaggactt
      961 ccgacgcttg ccgggcttcc tggatttagc gggcttccct gtcttgctgc tccggcctga
     1021 ctttttcgcc ttgccatgtt gaccgggctt cctcgaacgg cctggcttgc ctggttttct
     1081 gcggcgggtt ttggcccggc atgtttccag gtcgccaccc tcgctggcca ggcacgcttt
     1141 cagggcttct tcctcttcca ggcgctgcag ggcttcgttt tcctcgttgt gaggtgaaac
     1201 cttgttgcgg tgatcggcgc gcttctgcag gaagtaggcg ctccagtcca cctgcggctg
     1261 agcgagatag gcgtcgatga cgcggtaagc cagggcaaag ggcagaaggg agttgtgcat
     1321 attggtcagc actactacgc cgaaaccctg agccggaccc atcacgcaga aggaagtgaa
     1381 gccgtcgacg tagccgccgt gttgcacgag atgggcgccg ccgtagtcgc gcacgaacca
     1441 ggtcatgccg tagccgtggc gggttgagcc gggatgcatc atgagggtga aggcgttggg
     1501 ctccacctga agatgctcgc ggtgcatctc gtcgaggtcg ctcttatcga gaatttcccg
     1561 gccgatggta cccagctgga acttcagcca gttggccata tcgtcgatac ttgactgtaa
     1621 ggctgcggaa gaagccaggt agtcgagaga cttcaccttc atgatagccg ggcgcttgcg
     1681 gttgcgcggc atgtggtgag gaacggcgcg gttgcgacgc cttgccgagg ctttcaccga
     1741 accgaagctg tccttcatac ccaggggtcg caggatgcgg ctctgcagaa agtcttccca
     1801 actcatgcct gagaccttct ccaccacaag ggccagcacg gccagaaggg tattgttgta
     1861 gctgaagccg ccccggtgcg gtcttgtcgc ctgcagtccg gccacatgcc gcatggcttc
     1921 ctgcaaggtg cgggagggca gattggtgat ggcgccgtct tccacaccgc tctggtgagt
     1981 gacgacgtcc ttgatggtaa gggctctgcc cactgccgcg tcggccaggg aaaattccgg
     2041 cacatatttt tgcaccggat cgtcaaacga gatcttcttc tcccgggcaa gaatggccat
     2101 ggctaccgtg gtgaaagcct tggtgcagga gccgattgtg aaaatggagc gacggttgac
     2161 gggacgtccg tcgcctttct tgagcaagcc gaagccgtct gcaaagacta cttgtccgtc
     2221 cttgacgaca gcgatggaaa gacccggtac gtcccagtct ttcatggctt gttttatgta
     2281 agttacatcc agaccgaagt cttgatcggt gttcgacata gacatgtcct ttgccaaaga
     2341 taacggccgg tgccttatct ggcgccggct gagagtggtt atttaaaact gcaaggtgag
     2401 cggaaaaacc gtcaggcaca gcggcttcgt tcagcaagtg ggtatgactt gcctaccggc
     2461 tgaactggta gcagcacttg ggggcggttg gtagcgagcg tgtttttccg atactgcgta
     2521 atgaaagaaa gtggaattga tgtgccttag tttctaaatt acatggtcca agctcaaagg
     2581 ctatttatgt aattccggtt catatttatg caaactaatt tgccgaccac cgtttcgttt
     2641 aaactatggc acgcctagcg ctagtattcc gcttgacgac ttgccggcat ggccgttttg
     2701 aaggtcgcat cgaac
//
LOCUS       DAOQNZ010000459         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20916_length_2711_cov_3.816642, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000459 DAOQNZ010000000
VERSION     DAOQNZ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20916_length_2711_cov_3.816642"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PKW73_09070"
     CDS             <1..820
                     /locus_tag="PKW73_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNA73478.1"
                     /translation="FTPDLSLVQDLPLAAKAHIVLAWVLILLVPFSRLIHMFAVPLEY
                     IGRPWQNVVWVSDRRKEAAPQAFVEGEARRYFVKGGLAAVFGLSLLSVGVFDKIFRFF
                     FGPRLTAHQETELMTTRLSRLEATSSQRKLELERQENDYILVGSLAELSSEKGKYFID
                     YSMMPALAFKTAEGLPLLISAKCTHLGCTVGADAVDGKILCPCHISYFSIDTGMPNAD
                     SPAKTPLPHLGWVVMDTRGNQLASFSGNGAVKGTISEAQRTSARVYIAKAHQIG"
     gene            942..1976
                     /locus_tag="PKW73_09075"
     CDS             942..1976
                     /locus_tag="PKW73_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNA73479.1"
                     /translation="MNLEHMLAHKEVPVHKMSWGYYTGGMTMFFFMIQVITGLFLLFY
                     YQPTVSDANLSVEYITKYVSVGGLIRNMHAWSASGMIFFAVVHLITAFAMKSFEKPRE
                     LTWIMGVLLLIITFTFGFTGYLLPWNQIAVNATKVGLQSVEDIGQYLPGMLHDVPRIL
                     RETFQGEASVGQSTLGRFYALHVVLLPLALAGLLGIHLLQVQVHGMSQGVDEKPKKQE
                     RFFPVFLFKDLTLWGVLFFILFTFSLCIPFESFLPFPLLAPYDAMGATPPGIKPEWYF
                     FFVYYPLELLPFWVILLASGLIGAVLIFTPWIFKGSSRKTLGFIATAVSLYLLIMTLF
                     GEQIYHLLKH"
     gene            1986..2600
                     /locus_tag="PKW73_09080"
     CDS             1986..2600
                     /locus_tag="PKW73_09080"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNA73480.1"
                     /translation="MNISFSRTSKTLIAVTPVLIWLFAMPQTQAYPQFQEFSEKNSGR
                     NVNCAMCHVNGDGPTGDGKGQIGGLTKEELDSLMKARSALAPGTLVDSPILNKFGNEI
                     IKDIGKRKFLELRQNPKDLAKALPAQSDLDQDGISDGQEYLDGTDPLNPEHGDPLKLL
                     MANLNKYRLHVILTFIAVMTLNWGIMQFLKGLEILTKPRQIPKA"
BASE COUNT          654 a          775 c          656 g          626 t
ORIGIN      
        1 cttcacaccg gacctgagcc tggtacaaga tctgcccctg gctgccaaag cccatatagt
       61 gctggcctgg gtcttgatct tactggtgcc gttttcccgc ttgatccata tgtttgccgt
      121 gccgcttgag tacatcggtc gcccctggca gaacgttgtc tgggtaagcg accgccgcaa
      181 agaagccgca ccgcaggctt ttgtagaggg tgaggcgcgc cgctattttg tcaagggagg
      241 actggcagct gtgtttggtc tgtcgctcct ttcggtaggc gtcttcgaca aaatcttccg
      301 cttcttcttc ggtccgcgtc tgacggccca tcaagaaaca gaattaatga ccacccgcct
      361 gagccggttg gaagccacct ccagtcaacg caaattggaa ctggaaaggc aagaaaacga
      421 ctacatcctg gtaggcagtc tggccgagtt gagttccgag aaaggcaaat acttcatcga
      481 ctactccatg atgccggctc tcgccttcaa gaccgcagaa ggactgcccc tattgatttc
      541 cgccaagtgc acacacctgg gctgcaccgt gggcgccgat gccgtggacg gcaaaattct
      601 ctgcccctgc catatcagct acttcagcat cgatacgggc atgcccaacg ccgactcccc
      661 ggccaagacc cccctgccgc atctgggctg ggtggtcatg gatacccgag gcaatcagct
      721 ggccagcttc agcggcaatg gagcggtgaa gggaactatc agcgaagccc agcgcaccag
      781 cgccagagtt tacattgcca aggcgcatca aatcggatag gtggaacctt caatgagagc
      841 tagtttcaaa aggacactag aaaatgacaa gggataactc aggtataagc aaaacggcaa
      901 gcgcacttag cttcattgcg gatcgctttc cgcttgagaa aatgaacctt gagcacatgc
      961 tggcccataa agaagtaccg gttcacaaaa tgtcctgggg ttactacacc ggcggcatga
     1021 ccatgttctt ctttatgatt caggtcatta ccgggctctt cctgcttttc tactaccagc
     1081 cgacagtctc cgatgccaat ctttcggtgg agtacatcac caaatatgtc agtgtcggtg
     1141 ggctgattcg aaacatgcat gcctggtccg cttcgggcat gatcttcttt gccgtggtgc
     1201 acttgatcac ggcttttgcc atgaagagct ttgaaaagcc aagagagctg acctggatca
     1261 tgggagtact gctcctgatt atcaccttta ccttcggctt caccggctac ctcttgccct
     1321 ggaatcagat tgccgtcaat gccaccaagg tagggctgca gtcggtggaa gacatcggtc
     1381 aatacctgcc cggcatgttg cacgacgtac cgcgcatctt gcgtgaaact ttccagggtg
     1441 aagccagtgt cggtcaatct accctcggac gcttctacgc actgcatgtg gtgctactgc
     1501 ccctggccct ggccggtctc ctgggcatcc acttattgca ggtgcaggtg catggcatga
     1561 gtcagggcgt cgacgaaaaa ccaaagaaac aagagcgttt ctttcccgta ttccttttca
     1621 aggatctcac cctctggggt gtcctcttct ttattctctt caccttttct ctgtgcatac
     1681 cttttgaatc cttcctaccg tttcctttgc ttgcgcccta tgacgccatg ggtgccactc
     1741 cgcccggtat caagccggaa tggtatttct tcttcgttta ttacccgctg gaactactgc
     1801 ctttctgggt gattcttctg gcttcaggct tgattggcgc cgttctaatt ttcacaccct
     1861 ggattttcaa aggtagcagc cgcaaaaccc tgggcttcat tgccaccgcc gtttctctct
     1921 acttattgat aatgaccctc ttcggtgaac agatctatca ccttctcaag cactgacaaa
     1981 caatcatgaa catcagtttc tcccgaacca gtaaaacact aattgccgtc acgccagtgc
     2041 tgatatggct ttttgccatg ccgcaaactc aggcttatcc gcaattccag gaattctccg
     2101 agaagaactc gggcagaaat gtcaactgcg ccatgtgcca cgtcaatggc gacgggccca
     2161 caggagacgg caaaggacaa ataggtggtc tcaccaaaga agagctggac agcctcatga
     2221 aagcccgctc ggcgctggca ccgggcaccc tggttgatag ccccatcctc aacaagttcg
     2281 gcaacgaaat cataaaagac attggcaaac gaaagtttct ggaactaaga caaaatccaa
     2341 aggacctggc gaaagcactg cctgcgcaat cagatctaga ccaggacggc atcagtgacg
     2401 gccaggagta tctggacggt accgacccct taaaccccga gcatggcgac ccgctcaaac
     2461 tgctaatggc caatctcaac aaatacagac tgcatgtcat cctcactttc atcgccgtaa
     2521 tgacactaaa ctggggcatc atgcaattcc tcaaaggtct ggaaatactg accaaaccgc
     2581 gacaaatacc caaagcttaa gcggacgccc ttaaagatca ctgatggagt ccacagaaaa
     2641 tggcaagcat gactcaagca aaagaaccaa ccggcagttc cgattcaaga accggcgccg
     2701 atttgagagg c
//
LOCUS       DAOQNZ010000460         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_20927_length_2710_cov_3.964595, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000460 DAOQNZ010000000
VERSION     DAOQNZ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20927_length_2710_cov_3.964595"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1391)
                     /locus_tag="PKW73_09085"
     CDS             complement(195..1391)
                     /locus_tag="PKW73_09085"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA73481.1"
                     /translation="MNQSKYESYEVIIIGAGGAGLMCAIEAGKRGRRVLIIDHNREVG
                     RKILISGGGRCNFTNLGANPDCYVSSNKHFVKSALSRYTPQDFIKLVQKHHIRYHEKK
                     LGQLFCDESASQIVDMLVKECRQAGVAFALGEKIRSVKKDPDQGFLIEGETKEWQCLS
                     LVIATGGLSIPQIGATGFGYDIARQFGLKITELAPALDGFDWRQGDLKGFTELAGISL
                     DVEISVNKVSFRENILFTHKGLSGPASLQASLYWKRDNSLSINLLPTMDAGQYLLEQK
                     QKRGKQEVKNVLSDVLPKRFAEYFTEHLHVAGPLASLDGKRLQLLAANLNNWQVKPEK
                     TVGYKKAEVTRGGVDTAALSSTTMETKSVPGLYFIGEVVDVTGRLGGYNFQWAWASGY
                     AAGQSV"
     gene            1508..1927
                     /locus_tag="PKW73_09090"
     CDS             1508..1927
                     /locus_tag="PKW73_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73482.1"
                     /translation="MSQEDTKLESCEGKTTSNPEQVQSLVRGERLLRAQSSLGAFAVY
                     FESGRALVLQGAMSEFGPDIEVELKPSTLVPEQSEAVCSVNWSWIYGRTVKNLKKRTG
                     GMGAAYGLDLGDGLVITISVGLWEGKPFLSFMPYKPQ"
     gene            complement(1931..>2710)
                     /locus_tag="PKW73_09095"
     CDS             complement(1931..>2710)
                     /locus_tag="PKW73_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73483.1"
                     /translation="SQLLQSKFTSHIYGFFSLALALALTDAAAFAYTIEGGVEHVERM
                     PSVAPELRAGADFHEDKLTNGASNRWLKVPNWLAGTWLVKTETAVFQKDYKTGRSSNS
                     QTTYKARHEFTYGYQKDLQGDIWHYIGVPYTSKTSLSEYDEFHKVSLKDFQTVTGNEV
                     RFRTLMTVIRVNRSSDTIITSFQQESITSYRPVEDGVIELTSSTKSFDAAGNPRHETC
                     NTATIKRVKPYIAVNEKDGKDLAALFKDFLVSRGLTNLLPQ"
BASE COUNT          583 a          655 c          771 g          701 t
ORIGIN      
        1 aggcgggtag aactttgggt acaagaaaat gtctgcatga tcacccccgg gggtgattag
       61 tgagaatgga tggataactc tgcgccgttt cctaagatac tttgtagtat cactcagtac
      121 taacatctta gcggaaagtg ccgctttggg atagaaagcc ttgggaaagc cggccggtac
      181 cgggaggcat tttattaaac cgactgcccg gcagcatagc cggaagccca ggcccactgg
      241 aagttatagc ctcccagacg tcctgttacg tctaccactt cgcctatgaa atataggccc
      301 ggtactgatt tggtttccat ggtagtggag gagagggcgg cagtgtctac accgcctctg
      361 gtgacttccg ccttcttgta gcctacggtt ttttcaggct tgacctgcca gttatttaaa
      421 ttggcggcca ggagttgcag gcgcttgccg tccagggaag ctagtggacc ggcaacatgc
      481 aagtgctcgg taaaatattc tgcaaaacgt ttgggtaaga cgtcgctcag tacgttcttc
      541 acctcttgtt tgccccgctt ttgtttctgc tcaagcagat actgcccggc atccatggtc
      601 ggcaaaagat tgattgagag gctattgtcg cgcttccagt agagtgaagc ctgcaaactg
      661 gccgggccgc tcagaccttt atgggtgaag agaatgtttt ctctgaagct gactttattg
      721 acgcttatct ccacatccag ggatataccg gccagttcgg tgaaaccctt caagtcaccc
      781 tgccgccaat cgaagccgtc cagggccgga gccagctccg taatcttcaa gccgaattgg
      841 cgggcgatat cataaccgaa tccggtggca ccgatttgcg gtatggagag tcctcctgtg
      901 gcgatgacaa ggctcaagca ctgccactcc ttagtctcgc cttcaatgag gaagccctgg
      961 tccggatctt tcttgacgga ccttattttt tcaccgagag caaaggctac cccggcctgg
     1021 cgacattctt tcactagcat gtctacaatc tggctggcgc tctcgtcgca gaaaagttga
     1081 cctaatttct tttcgtggta gcggatatga tgcttttgca ccagtttgat gaagtcttgc
     1141 ggtgtatagc gtgaaagagc tgatttgaca aagtgcttgt tgctcgaaac gtagcagtcg
     1201 gggttggcgc cgagattggt gaaattgcag cgtccgccgc cggatatgag aattttccgc
     1261 cctacttctc ggttgtgatc gataatcaaa acgcgacggc cgcgcttacc ggcctctatg
     1321 gcacacatca gaccggcacc gccggctccg atgatgatta cttcgtagct ctcgtactta
     1381 ctctggttca aggttgactt tcgctttttg ataaaaggct ttttattaaa tgcctcaaaa
     1441 ggctgacttc ttacggtcat tctgggctta atatgcacct ttacggataa ggaattttag
     1501 caaagctatg agccaggaag atactaagtt ggaaagctgt gagggaaaga ccacctctaa
     1561 tcctgagcaa gtgcagagtc tggtcagggg tgaaaggctg cttcgcgctc aaagcagcct
     1621 gggggccttt gccgtctact ttgaaagcgg ccgggctctg gtcttgcagg gggccatgag
     1681 tgaattcgga ccggatattg aagtggagct taagccgtct acccttgtgc cggagcagtc
     1741 ggaagcggtt tgttcggtaa actggagctg gatctacggg cgcaccgtga agaacctgaa
     1801 aaagcgcact ggcggcatgg gggcggccta tgggctagat ctcggcgatg gtctggtgat
     1861 caccatcagt gtcggtctct gggagggcaa gcccttcctg tcgtttatgc cttacaagcc
     1921 ccagtagcag ttattgcggt agtaagttgg tgagaccgcg gcttaccagg aagtctttga
     1981 agagggcggc caggtctttg ccgtctttct cattgacggc gatatagggc ttgacccgct
     2041 taatcgttgc cgtattgcag gtctcatggc ggggattgcc ggcggcatcg aaagatttgg
     2101 tggaggaggt cagttcgatg acgccgtctt ccacggggcg gtaactggtg atgctttctt
     2161 gttgaaagct ggtgataatc gtgtcggaac tgcgattgac cctgataacg gtcatgaggg
     2221 tgcgaaagcg cacctcattg ccggtgacgg tctgaaagtc cttgagggaa accttgtgaa
     2281 attcgtcgta ttccgaaagg ctggtcttac ttgtataagg gacgccgatg tagtgccaga
     2341 tgtcgccttg caggtctttc tggtagccgt atgtgaattc atggcgggcc ttgtaggtgg
     2401 tctggctgtt gctgcttctt ccggtcttat aatctttttg aaagacggcg gtttcggttt
     2461 taaccagcca ggttccggcc agccagttcg gcaccttgag ccagcgattg ctggcaccat
     2521 tggttagctt atcttcgtga aagtctgccc cggctcgcag ttccggcgcc acagaaggca
     2581 tgcgttccac atgttccacc ccgccctcga ttgtgtaggc gaaggcggct gcatctgtta
     2641 gagcaagggc aagagccagg ctgaaaaagc cataaatatg actggtaaat tttgactgga
     2701 gcaattgcga
//
LOCUS       DAOQNZ010000461         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21034_length_2704_cov_2.812760, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000461 DAOQNZ010000000
VERSION     DAOQNZ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21034_length_2704_cov_2.812760"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PKW73_09100"
     CDS             complement(<1..1196)
                     /locus_tag="PKW73_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73484.1"
                     /translation="MEQLPQKKGSVFGFLTNLFSGGSGGAKPKQKKAEPQNGKGKVKD
                     KGPDKGKFKGKGKGQESDFEPENDDIDQTPSPVGRGGMKFQRKTKEVDQEEIESQVGG
                     FSRLKSIGTKAQEQENREAQSTTRSLGEKFSMPWAAQSGMDEGPLTLAESIKRKVTES
                     QKLSPLDEDDLSGSQNELTGGGFAAPAQSSSFAPSTAAAFSQPSGFNQPSGFSQPEES
                     GNEAWGAPVNMPNKQPVKLGAPGPSPTPVGFAEPSGFASNQNVPAQTPVGFGAPSDSE
                     HRGSAIGLAPKQQEPQAKAPASDWGGSWAPEGGASVENKANWGGTAGNANASGSGAAI
                     NPPQEAPKADWGSGWNAPTDARQPLKSSESWAIPGSEGAGSWNSTPEPHAREAEASPA
                     PAPAPAP"
     gene            complement(1482..2060)
                     /locus_tag="PKW73_09105"
     CDS             complement(1482..2060)
                     /locus_tag="PKW73_09105"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA73485.1"
                     /translation="MPLLPAKNSTQEFDQSAQAVLADLKKGGSEKRNEFVWIQQERVF
                     AVALLGTGSQESAAQVTISAFNNAFAALNHSLPKNPEMSVWDWLSQYVVEAMGEFHNE
                     FSGAPDDEDADPAQDGSANMDWETTVLLGVQRVKRCINQLPQDQKRVFLLRHSLDLNY
                     DQISIVTNQKVEKCIALLYRARVQVVKCLGRG"
BASE COUNT          534 a          703 c          744 g          723 t
ORIGIN      
        1 ggagcaggcg ccggtgccgg tgcagggctg gcttccgctt ctctggcgtg gggctcggga
       61 gtgctgttcc atgagccggc accctcggaa cctggtatgg cccatgattc tgaagacttg
      121 agaggctgtc tggcatcggt gggggcattc caacctgaac cccagtcagc tttaggggct
      181 tcctgtggcg gattgatcgc cgccccgctg cccgaagcat ttgcattacc ggcggtgccg
      241 ccccagttgg ctttgttttc gaccgaggct cctccttccg gagcccagct tccgccccaa
      301 tcacttgccg gcgctttcgc ttgcggttct tgctgtttgg gggccaggcc aatggcgctg
      361 ccacggtgtt ctgaatcgga gggagcgccg aacccgactg gggtttgagc cggtacattt
      421 tgattggagg cgaagccgct tggttcggca aagccgacgg gagtgggaga tggaccgggg
      481 gcgcccaatt tgacaggctg tttgttcggc atattgaccg gcgcgcccca ggcttcattg
      541 ccgctttctt ccggctggct gaagcctgaa ggttggttga aaccggaggg ctgactgaag
      601 gccgcggccg tactgggagc aaagctgctg ctctgggccg gggcggcaaa accaccgccg
      661 gtgagctcgt tttgagagcc ggagaggtcg tcttcatcga ggggggaaag tttttgagac
      721 tctgtcacct tgcgcttgat ggactcggcc agagtgaggg gaccttcgtc catgccgctt
      781 tgagcggccc agggcataga gaatttttcg cccagggagc gagttgtgga ttgcgcttcc
      841 ctattttcct gttcctgagc ttttgtgcct atggatttaa gcctggagaa accaccgacc
      901 tggctttcga tctcttcttg atccacttct ttggtcttgc gctggaactt catgccaccg
      961 cggccgaccg gactgggagt ttgatcaatg tcgtcgtttt cgggttcaaa gtcagactcc
     1021 tggcctttac ctttgccctt aaatttgcct ttgtccggtc ctttgtcctt gaccttgcct
     1081 ttgccgtttt gaggctcagc ctttttctgt ttcggtttgg caccacctga tccgcctgaa
     1141 aacaggttgg tgaggaaacc gaaaactgaa ccttttttct gaggaagttg ttccatgatc
     1201 attctcgcaa gggcttcggg attctgtgga cggagttcat ctggcagcgg ggtatcttgc
     1261 gctgcttgaa tcatctgatc caattggtgg tgaaatttct ggccggattc tgacccttcc
     1321 aggtgatcaa tgagggcgcg gtgctcttgc ttgctgagct cctcgtcaaa tgaccgttgg
     1381 attagtaatc gaaattgcgc gtcagacatt ccttatttaa acctttttgt ctaggcggac
     1441 actaattctc tgttatcgac tctctgggaa tttgcttgct gtcagcctct tccaaggcat
     1501 ttgactactt gtactctggc tctgtagagc agggctatgc acttttcaac tttctggttg
     1561 gtgactattg aaatctggtc ataattcagg tcaagagagt gacggagcag aaatactctt
     1621 ttctggtcct ggggaagctg gtttatgcag cgcttgactc gctgtacgcc cagcaagaca
     1681 gtggtctccc agtccatatt ggcagagccg tcctgggcgg ggtcggcatc ttcgtcgtcg
     1741 ggggcgccgg aaaattcatt gtgaaactcc cccatggctt ccactacata ctgagaaagc
     1801 cagtcccaga ctgacatttc cggatttttc ggaagggaat ggttgagagc ggcaaaggca
     1861 ttattgaagg ctgaaattgt tacctgggcg gcgctttcct ggctgccggt acccagaagt
     1921 gccacggcaa aaacccgttc ttgttggatc caaacaaatt cgttacgctt ttctgagccg
     1981 cccttcttca ggtcggccaa tactgcttgc gccgattggt cgaattcttg agtactgttt
     2041 ttggctggta gaagaggcat taaatactat tcctgacccg cgaggtcgac gtttggaagt
     2101 aacgaaccca ttttcaggtg tcgccggctt tctgtcaggc tatcttcaca ctcaacgatg
     2161 gtagacaaaa tccgatagat agaaaaatcc ccacctcacc cgcaattaaa ccaaaatgga
     2221 agcgggcgca accaagacgc ctgttatcag tttacctgat atgcggccac tgaggtagag
     2281 ccagtcagac ttttcctgca atttacctcc caatttgtcg tctgctggta cactaactgc
     2341 ctaggccgtt aacatagatg taccacgttt gaccataaag caaaatggca gccgcaatct
     2401 ccgcaatact gataaaaaat cgcgaccctg aagcttacaa tctgggcacc attgtccaga
     2461 atccacgcgg ctgtacgtcg gttgatctga atgcagcctg tagtaatctc gtttttgagc
     2521 cttccggccc gatggcaaac agtgagcaca gtgccatcgt tctcgacctg gtgcgctatt
     2581 tattccagga cttttttaat cgctcccatc aaaccggact ctacaacagg cagatgcgcc
     2641 tctggcaagt cttttccaag gtggagagag tggagtttaa gcaactggag ggcggctttt
     2701 ttac
//
LOCUS       DAOQNZ010000462         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21124_length_2698_cov_2.594400, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000462 DAOQNZ010000000
VERSION     DAOQNZ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21124_length_2698_cov_2.594400"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKW73_09110"
     CDS             complement(<1..514)
                     /locus_tag="PKW73_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73486.1"
                     /translation="MSISTIGFADLDYNSLYAIPLGGQSEMGLVLWAFIHQGKILLVD
                     AGASYPAHDLPGVDLLLPSTTFLEANQEHILGQVLTNGHEEHMGAIAYLIQHVKLPRV
                     LCPKFVANLIEQEVFDLYGTGHIEKSDTTIEVVDQKKEYRVGPFSLEWIECNNSIADA
                     CALRISCQGGT"
     gene            630..2216
                     /locus_tag="PKW73_09115"
     CDS             630..2216
                     /locus_tag="PKW73_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNA73487.1"
                     /translation="MKADSVNVKDGAGAALETLVGALVNPDCKIPHKAVIYTKAGGDV
                     EYSFAEFASLINKAETLLKNLGIKADDPVVFLSSNTPELLAAILATFRLGALAAPVDF
                     RLTQGELVNIARKLKAKVVLSLESLHKDFAALKGELSGSGIALESLNKIESQQESTRN
                     GGNCDYESLAKPAFLILTSGTTGMPKGALHDLASLAANIKELADMAALEPGYKVVLPV
                     PLSHVLGLEVSLAALLKQTTIVMSEMTIEGIIKANNLFKPEFLVGVPTIYGAILTLPE
                     GAIDLQNARVLLCGGAPLPLSLAEEFEKKYQRRLNNGYGSTESKIIAVNLVGPPQSVG
                     KVVPSCQIKILPLTAEDGKPVEPLPEGVEGEIVICGPTLMLGYIGQPEETARVMQDGG
                     YKTGDIGYMKDGYLYVLGRNKEMIIVAGNKVFPAEVEGVLRQCPLVKEVAIIGVAHSK
                     LGQIVKAHIVTTDGDLSQGLEGDEEAKKAAHSKLKEGLREFCQTNLKRELRPMEWVFY
                     SKNSQLPRTNTGKIDKKVLA"
     gene            2301..2669
                     /locus_tag="PKW73_09120"
     CDS             2301..2669
                     /locus_tag="PKW73_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNA73488.1"
                     /translation="MAVKSGSFVQHQWNAQSPVFARAMCMAKGTVFVSGPPDEIDEEY
                     AFERMSKKDPAVYKELAEQDAALAGERGGSLLAVNTEKGNIGGEVKLDSPPVWDGMAI
                     AQGRLYVASQDGKVRCFGRK"
BASE COUNT          679 a          754 c          732 g          533 t
ORIGIN      
        1 ccgttccccc ctggcaacta attctaagag cgcaggcatc ggcgatggaa ttattacatt
       61 cgatccactc cagactgaaa ggtcccaccc ggtactcttt cttctgatcc accacctcga
      121 ttgtggtgtc ggacttttca atatgtccgg tgccgtagag gtcgaagacc tcttgttcga
      181 ttaaattagc cacaaactta ggacagagca cccttggcag tttgacgtgc tggatcagat
      241 aggcgatggc tcccatatgc tcttcatgcc cattggtaag cacctgcccc agaatatgct
      301 cctggttggc ttctagaaaa gtggtactgg gcaaaagcag gtcgacgcct ggcaagtcat
      361 gggccggata gctggctccg gcatccacca ggaggatctt gccttgatga ataaaagccc
      421 acaacactag ccccatctca ctctgcccgc ccagcggtat ggcatatagg ctgttgtagt
      481 caaggtcggc aaagccgata gtagaaatag acaagacggg accccgatga ctggccagga
      541 gttgctaaag tgtttgcatg cccaacaatt ctaaccgcaa tccagttaag atctatgcaa
      601 atcggcaaga gggttgaaag cagttaacta tgaaagcaga tagcgtgaat gtaaaggacg
      661 gcgccggcgc cgcactggaa actcttgtgg gcgccctggt caatcccgac tgcaaaattc
      721 cccacaaagc cgtcatttac acaaaagccg gcggagatgt ggaatacagc tttgccgagt
      781 ttgcaagcct cataaacaag gccgaaactc tcctgaaaaa tctgggcatc aaagcagacg
      841 acccggtggt cttcctttcc agcaacaccc ccgaacttct agcggcaatc ctggcaactt
      901 ttcgcctggg ggccctggct gcgccggttg attttcgcct tactcagggc gagctggtca
      961 atatagcccg caagcttaag gcgaaagtgg tcttgagcct tgagagcctg cataaagatt
     1021 ttgctgctct caaaggcgag ctttcaggat caggcattgc cctggaaagt ctgaacaaaa
     1081 tcgagagcca gcaggaaagc accagaaacg gtggcaactg cgactacgaa agtttagcga
     1141 agccggcctt cctcattctt acatccggca ctacgggcat gcccaaaggt gccctgcacg
     1201 acctggcttc actggcagcc aatatcaaag aactggccga catggccgcc ctcgaacccg
     1261 gctacaaagt agttttgccc gtacccttga gccatgtgct ggggctggaa gtttctttag
     1321 cggcccttct caaacagaca accatagtta tgtcggagat gaccatcgag ggcatcatca
     1381 aagccaataa cttatttaag ccggagtttc tggtgggggt acccaccata tatggtgcca
     1441 ttcttaccct gcccgagggc gccatcgacc tgcaaaatgc ccgggtcttg ctttgcggtg
     1501 gggcgccatt gcctctttct ctggcagaag aattcgagaa gaagtatcag cgccgcttga
     1561 ataacggcta tggctccacc gaatcaaaga tcatcgccgt caacctggtg ggtccgccgc
     1621 agtccgtagg caaagtggtg ccctcctgcc aaattaagat attgcctctg acagccgaag
     1681 atggcaagcc ggtagagcct ctgccggaag gtgtagaagg cgaaatagtt atctgcggtc
     1741 caaccctcat gctcggctat atcggacaac cggaagagac ggccagggtc atgcaggacg
     1801 gcggatacaa aaccggcgac atcggctaca tgaaagatgg ttatctttat gtgcttggcc
     1861 gcaacaaaga aatgattatt gtggccggca acaaagtctt tcccgccgaa gtggaaggcg
     1921 tgctcagaca atgccccctg gtgaaagaag tagccatcat cggtgtggct cacagtaaac
     1981 tcggtcaaat tgtgaaagcc catatcgtca ccaccgacgg tgacctatcg caaggtctgg
     2041 agggcgacga agaagcaaaa aaagctgccc atagcaaatt gaaagaaggt ctgcgcgagt
     2101 tttgccagac caacttgaag cgcgagttgc gccccatgga atgggtcttc tatagcaaga
     2161 acagccagtt gccccgtact aataccggca aaatcgacaa gaaggtgctg gcttaattaa
     2221 agcctcgaca atatgcgcca gccgccggca ttctcgccgg cggcaaaaac gcggctggcg
     2281 acaaagacac cgccgccgcg atggcggtga aatcgggcag cttcgtgcag caccagtgga
     2341 acgcgcaaag ccccgtcttc gcccgcgcga tgtgcatggc caaaggcacc gtcttcgtct
     2401 ccggcccgcc cgatgagatc gacgaggaat acgccttcga gcggatgagc aaaaaagatc
     2461 ccgcggtcta caaagagctc gccgagcaag acgccgctct cgctggcgaa cgcggcggca
     2521 gcctcctcgc ggtgaacacc gaaaagggga acatcggcgg cgaagtgaag ctcgacagcc
     2581 cgcccgtttg ggacggcatg gccatcgccc aaggccgcct ctacgtcgcg agccaggacg
     2641 gcaaagtgcg ctgcttcggc cggaagtagc gcgcggctca gcgcaccgcg gcgagtgt
//
LOCUS       DAOQNZ010000463         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21147_length_2696_cov_3.482014, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000463 DAOQNZ010000000
VERSION     DAOQNZ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21147_length_2696_cov_3.482014"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PKW73_09125"
     CDS             <1..1291
                     /locus_tag="PKW73_09125"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73489.1"
                     /translation="HQTLGQVKIAQDDIEAAVDPLRKACNLAENCVNLTLLAQALLKL
                     DPKDKEADELVTKALQLKSDYPAARLQKAIVLSAQGKNEDAYLELKNIPPEHRNSEWH
                     TVAGNIESRQGDGPSALQSWNKASMEDPRNPEPYKRKAEHYVMRGDGALAIAEYHTGL
                     EILPNDWAMRGELAELALRQDNLDVAETEFKTILATKPDDAKALLGLARVGFRKYRKE
                     GNFPPNFNQLMDRLQNIITEQSVQGKVVKEGTRSLQESIELSEATKAISQNRFREGRQ
                     KFIDVIEKHKEEPYDLVTLGEQCIFEGDYKAAEKAFAYAKEIPEVSTRAEQGMSKITT
                     QRNEASRHVKLGDAAWKIPEIAIDHYKQALNADPQYPGAYFGLFSLYTKSDKQDPNQA
                     VTYAEGFLEVADDVSPQRREVEAGLAKLKKRTGGKGK"
     gene            1332..1601
                     /locus_tag="PKW73_09130"
     CDS             1332..1601
                     /locus_tag="PKW73_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73490.1"
                     /translation="MTAGEKKSVEAEEGKTIRRSYLQILRSSYAAMSSSEQDSLAQNF
                     YLVIAGGMALIALSTCGTDLPRHMMSVIGLPTLIALYFTVSRSKW"
     gene            1621..2043
                     /locus_tag="PKW73_09135"
     CDS             1621..2043
                     /locus_tag="PKW73_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73491.1"
                     /translation="MTAGKRKPGKGRFPSKGGTEDEVFDASPDKNSAPIIADGAKDLR
                     DTEAWKKGKSSVTGAWDRYLKILRDSFQNMDRYDQEVLITRFCYVISIGISGIILSCF
                     YGLLPAMVRVLALPIVLAGGWYVGSKIVAPAMIARIIK"
     gene            2089..2358
                     /locus_tag="PKW73_09140"
     CDS             2089..2358
                     /locus_tag="PKW73_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73492.1"
                     /translation="MTPKTKNILGGLVIMAAASLMLGHIFEYIAGLTRIISVILTVLA
                     VTWLAVFLLSKLSGKGYKDSKNGKSTSKNSTTDDITDAESRTVED"
     gene            2410..>2696
                     /locus_tag="PKW73_09145"
     CDS             2410..>2696
                     /locus_tag="PKW73_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73493.1"
                     /translation="MKFTFSLAANSQSGKNKSRLVALAVTVLVAGSANANWQANAQGD
                     FYKSSGGVSGKTAILPIGTSFEGRIQSTIGSTQSRAGERFAIEISAPVLGNG"
BASE COUNT          740 a          724 c          690 g          542 t
ORIGIN      
        1 ccaccagact ctgggacagg tcaaaattgc ccaggacgat atagaggctg ccgtcgatcc
       61 tctgcgcaaa gcctgcaacc tggcggaaaa ctgcgtcaac ttaactctcc ttgctcaggc
      121 cctgctcaaa ctcgacccca aagacaaaga agctgacgaa ctcgtcacca aggccttgca
      181 attgaagtct gattatcccg ctgctcgcct gcagaaagcc atcgtgctct ccgcccaggg
      241 caaaaatgaa gacgcttacc tggaattgaa aaacataccg cccgagcacc gcaattctga
      301 gtggcatacg gtggccggca atatcgagag tcgccagggt gacggtcctt ccgccctgca
      361 atcctggaat aaagccagta tggaagaccc ccgcaatccc gagccttaca aacgcaaagc
      421 cgaacactat gtcatgcgcg gcgacggcgc cctggccatt gccgaatacc ataccggcct
      481 cgagatattg ccaaacgact gggccatgcg aggggagttg gctgagttgg ccctcaggca
      541 ggacaatctc gatgtagccg aaaccgagtt caaaactatc ctggccacca agcccgacga
      601 cgccaaagcg ctgcttggtc tggctcgggt cggctttcgc aaataccgga aggaaggcaa
      661 cttccccccg aatttcaatc agctcatgga ccgcctacag aacatcatca ccgaacaatc
      721 ggtgcagggc aaagtggtca aggaaggcac ccgcagtctt caggagagca tcgaactctc
      781 agaagcgacc aaagccatct cccaaaatcg cttccgagaa ggtcgccaga aattcatcga
      841 tgtaatcgaa aagcacaaag aagaacccta cgatctggtc accctgggag agcaatgcat
      901 attcgaaggt gactacaaag ccgctgagaa ggcctttgct tacgccaagg aaattccgga
      961 agtgagcacc cgcgccgaac aaggcatgag caaaatcacc acgcaaagga acgaagcttc
     1021 gcgccacgtc aagcttggtg acgccgcctg gaagatcccg gaaatagcca tcgaccacta
     1081 caaacaggca ttaaacgccg acccccagta tccaggcgcc tattttggat tgttcagcct
     1141 ttacacaaag tctgataaac aagaccccaa tcaagccgtc acctacgccg aaggtttcct
     1201 ggaagtcgct gatgacgtca gcccccagcg ccgcgaagtg gaggccggtc ttgccaaact
     1261 caagaaacgc accggcggca aagggaagta gaatagtaag ggctctaatc tcgcgttaag
     1321 gagaaggtgg aatgaccgcc ggagaaaaaa aatcagtaga agcagaggag ggcaagacga
     1381 tccggaggtc ctacctgcaa atcctcaggt catcttatgc tgctatgagt tcgagtgagc
     1441 aagattccct tgcgcagaat ttttatttag ttattgccgg cggcatggcc ttgattgccc
     1501 tcagcacatg cggtaccgac ctaccccgtc atatgatgtc ggtgattggt cttcccacat
     1561 tgatagcgct atattttacg gtgtccaggt ctaaatggta aaagctaagg agaaggtgga
     1621 atgaccgccg gaaaaagaaa accaggcaaa ggaagatttc cctccaaggg tggaactgag
     1681 gacgaagttt ttgacgcctc gcccgataaa aatagcgcgc ccatcatcgc agatggagcc
     1741 aaggacctgc gcgatacgga agcctggaag aaaggaaagt ccagcgtgac gggagcctgg
     1801 gaccggtatc tgaagattct acgagactcc tttcagaaca tggatcgcta tgaccaggaa
     1861 gtcttgatca ctcgtttttg ctatgtcatt agtattggta tctccggcat cattttgagt
     1921 tgcttctatg gactcttgcc cgccatggtg agagtgctgg ctttgccgat tgtacttgcc
     1981 ggcggctggt atgtcggctc caagattgtg gcaccggcaa tgatcgctcg tatcatcaag
     2041 taatttccag gcatacatgt aatcagatta ggatagaaga caatggctat gactcccaag
     2101 actaagaaca tactgggcgg gctggtgata atggcagcag cctcgcttat gcttggacat
     2161 attttcgagt atatagccgg gttaaccaga ataatttcgg ttattctgac agtactggcc
     2221 gtgacatggc tggctgtttt cctcttatcc aaactaagcg gcaagggcta caaagattcc
     2281 aaaaacggca aaagcacctc caagaatagc accaccgatg atatcaccga cgccgaaagc
     2341 cgaaccgtcg aagactgagt caaacagtaa cttttcatcc ttgattccgc acttaccaga
     2401 gaaccgtcca tgaaattcac attctcatta gcagcaaact cccagtcagg gaagaacaaa
     2461 tcgaggctcg tggctctggc ggtcaccgtg ttggtagccg gcagtgccaa tgccaattgg
     2521 caggcgaatg cccagggcga tttttacaaa agctcgggcg gcgtgagcgg caaaacagcc
     2581 atcttgccaa tcggcacctc atttgaagga cgcatccaga gtacaatcgg ctcgacacag
     2641 tcgagagccg gtgaacgctt tgctatcgag atctcggcgc ctgtcttggg taacgg
//
LOCUS       DAOQNZ010000464         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21167_length_2695_cov_2.977273, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000464 DAOQNZ010000000
VERSION     DAOQNZ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21167_length_2695_cov_2.977273"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PKW73_09150"
     CDS             <1..1322
                     /locus_tag="PKW73_09150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73494.1"
                     /translation="QKLATTSDVKNAQEASSAYDEAIKKQQAADYDGAIPLYQKAISL
                     MPKESAYLYALATCYQAKGDFDNAIRTYQDAVSKAPSKDLAGYKQALASAQMAQAQPI
                     MDEAVQKHTAGDLAAAIPLYEKSLAMFPSNAHGWTNLAGAYQSNDDFANARRCYLKAV
                     DLDAKNESDNWYFAGLIDENYKRVPDAVSEYGKYLAAKPTGSYAADARARMQAIKLDP
                     SKCQVLATKAQVQATTAASAAFNEAVQLQTDKKYDEAIAKYQEALKSFPNEASYYYSL
                     GTCYQAKEDYDNAIAQYEKARNLNPKEPAYPQLIKQLKQAKAAPLVNSAIEKQTQKND
                     VPGAIADYEASLKIYDDATTHSYLGTAYQAQNNLQKAMSEYMRALSMDKTLVDTYYYL
                     GTVYEGLKQPAKAVEEYQKFMRSAPAGNANTAAVKERLKLLAPAGRK"
     gene            1467..2027
                     /locus_tag="PKW73_09155"
     CDS             1467..2027
                     /locus_tag="PKW73_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73495.1"
                     /translation="MDFRRDGDSAGEKSTGLNSVDVLKMAVPQDKVATAHEKESSVAT
                     LDFSPLDALSLKKVEPAIQESAEKVGAAACVGAALCAGGMAGIGYRVGVQAALGAAAG
                     AAAGSQMERASELGKAAAAGAFEAVAGGAARVGREILNGKPSDIKGRDAVKAVGEAAA
                     ALSGGVVGGAVVGGAYGALNKLWRDK"
     gene            complement(2142..>2695)
                     /locus_tag="PKW73_09160"
     CDS             complement(2142..>2695)
                     /locus_tag="PKW73_09160"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNA73496.1"
                     /translation="RKPILPYADVMGACLPLAQAIGRWGNFFNSEAYGLPVTADFPLR
                     LYIPPGSRTLQYRLYDFFHPTFLYEAVWDLALFAYLYFYFAKKNRGMPGMTFFLYLSG
                     YSVGRILIEQLRIDSVSYFYSYPVPLLVSAATLVFSLSAMLHIYNKHHNEDVPVPAPA
                     PVPAPTPEPEPETEEPSQPDQMG"
BASE COUNT          669 a          700 c          738 g          588 t
ORIGIN      
        1 cccaaaagct tgccaccaca tccgatgtga agaacgcgca ggaagcttcc tctgcctatg
       61 atgaagcgat taagaagcag caggcggcag attacgatgg tgccattccg ctctatcaga
      121 aagccatctc gcttatgccg aaagaatcgg cttatctcta tgctctggct acttgctatc
      181 aggctaaggg cgactttgat aatgccataa gaacttatca ggacgccgtc agtaaggctc
      241 cctccaaaga tctggccggc tacaagcagg ctctggcctc ggcgcaaatg gcccaggctc
      301 agcccatcat ggatgaagcc gtgcaaaaac atactgccgg cgatctggca gctgccattc
      361 cgctttatga gaagtctctg gctatgttcc cctccaatgc gcatggatgg accaacctgg
      421 ccggcgccta tcaatctaat gacgacttcg ccaatgccag aaggtgttac ttgaaggcag
      481 tggacctcga tgccaaaaac gagtcggaca actggtactt tgccggttta atcgatgaga
      541 actacaaacg tgtaccggac gccgtttctg aatacggcaa gtacctggcc gccaagccca
      601 ccggtagcta tgctgccgat gccagggccc gcatgcaagc cattaagcta gacccctcca
      661 agtgtcaggt gctggctacc aaggcacagg ttcaggctac cacagctgct tccgctgctt
      721 tcaacgaagc cgtgcaattg cagacggaca agaaatacga tgaggctatc gccaaatatc
      781 aggaggctct caagtctttc cccaacgaag ccagctacta ctacagcctc ggcacttgtt
      841 atcaggccaa ggaagactac gataatgcca tcgctcagta cgaaaaggca cgcaatctaa
      901 atcccaaaga gccggcctat ccgcagctta ttaagcagct caagcaggct aaggcggcgc
      961 ctttagttaa ttctgccatt gagaaacaaa cccagaagaa cgatgtgccc ggcgccattg
     1021 ccgattacga ggcctccctc aagatttacg atgatgccac cacccatagt tacctgggca
     1081 cggcctatca ggctcagaat aatctgcaga aagccatgtc ggagtacatg agagccttat
     1141 ctatggataa gaccctggtg gacacctact attatttggg tactgtctac gaaggtctca
     1201 agcaaccggc caaggccgtg gaagagtatc agaaattcat gcgctcggct cctgccggta
     1261 atgccaacac cgctgccgtc aaagagcggc tgaaactgct ggctccagcc ggtcgtaagt
     1321 agtgtcaacc agcctgtacc gcttaaacag gtttgaaaca tttctgacct aacctttcag
     1381 cacgaagcaa tttgtcgccc cggcaaattc acccctgctg acaaatactt ttatgtctgc
     1441 catcctcggt ggcgggaggt tttcttatgg attttagaag agatggcgat agtgccggcg
     1501 aaaagagcac cgggctgaat agtgttgatg ttttgaaaat ggctgtgccg caagataaag
     1561 ttgcaaccgc ccatgaaaaa gaatcgtctg tagccaccct ggatttttct ccccttgatg
     1621 cactttccct caagaaggtg gagcctgcca tccaggaaag cgcagagaag gttggtgctg
     1681 ctgcctgcgt cggtgctgcg ctttgtgccg gaggtatggc tggcattgga tacagagtcg
     1741 gcgtgcaggc ggcgctcgga gcggctgccg gagctgccgc cggttctcaa atggagagag
     1801 cctccgaact aggtaaagca gccgctgctg gtgcattcga agctgtggcc ggtggtgccg
     1861 ctcgcgtggg cagggagatt ttaaacggta aaccttcaga tattaaaggg cgggatgccg
     1921 taaaagctgt cggtgaagcg gctgccgcgc tctccggtgg agtcgtcggt ggagcagttg
     1981 ttggtggcgc ctatggtgcc ttaaataaac tctggcgcga caaatagtcg tgacccaggg
     2041 gcagttttaa cgggcactct ggaattcatg ccctaatgtc ttaccggtgg gtcatattcg
     2101 cagctcggca gcgagtatga cccttccaac tttctcgtcc ttcagcccat ttgatccggc
     2161 tgagaaggtt cttctgtttc aggctccggc tcgggtgttg gcgccggtac cggggctggt
     2221 gctggtactg gtacatcttc gttatgatgc ttgttgtaaa tatgcaacat ggccgaaagg
     2281 gaaaacacga gagtagcggc tgacaccaga agcggtaccg gataagaata gaaataagag
     2341 acgctgtcga ttctcaactg ttcaataaga atgcgtccta cggagtaacc ggagaggtag
     2401 aggaagaaag tcatgcccgg catgccccgg ttcttcttag cgaaataaaa atataggtag
     2461 gcaaagagag ccagatccca caccgcttcg taaagaaaag tggggtggaa gaaatcgtaa
     2521 agccggtact gcagggtgcg gctacccggc ggaatataga gcctgagggg gaagtcggct
     2581 gttaccggca gtccgtaggc ttctgaattg aagaaattgc cccagcgtcc gattgcctgg
     2641 gccaggggca ggcaggctcc cattacatcg gcataaggca gaattggttt cctct
//
LOCUS       DAOQNZ010000465         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21171_length_2694_cov_4.518378, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000465 DAOQNZ010000000
VERSION     DAOQNZ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21171_length_2694_cov_4.518378"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..364
                     /locus_tag="PKW73_09165"
     CDS             239..364
                     /locus_tag="PKW73_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73497.1"
                     /translation="MLVYKLLWRSQGKADWMEECIVTVVESEKDDGTAACDTIAT"
     gene            514..1572
                     /locus_tag="PKW73_09170"
     CDS             514..1572
                     /locus_tag="PKW73_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HNA73498.1"
                     /translation="MKKGLLNQVVVLALAALAPAFSDAGALACTRTVCFGKDDIVVTA
                     RSLDWMEDMHSNLWAFPRGMKRNGAAGKNSLTWVSKYGSIVTSGYDAGTADGLNEKGL
                     AANVLYLVESDYGKGDGGKPAISIGAWAQYVLDNFATTKEVVAALSKEPFRVIAPTLP
                     NGVPANLHLAVSDQSGDSAIFEYIGGTLKIHHDKSYQVMTNSPDFDQQLALNSYWQKL
                     SGQTFLPGTHRAADRFARASFYINSIKESKDEDEAVAQSFSVIRSVSVPLGIATSGQP
                     NISSTIWRVVADHKRLVYFFDSAMSPNVFWVKMADLDLKEGAPVKKLTLTEGKTYSGN
                     AAGFFQKSKPFEFLPADQ"
     gene            complement(1594..>2694)
                     /locus_tag="PKW73_09175"
     CDS             complement(1594..>2694)
                     /locus_tag="PKW73_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73499.1"
                     /translation="LDVNEHKPEQLAEVLEKSSEPAHSFTHVQDLVRDMERCGDVYKL
                     SLAGGEDIIADTVVLALGNLPGRRPNWLHKLPLEDRHYIHDPWSRERWSQVQTAGSDI
                     LLVGTGLTAVDKVLELTKETRSSPRRIVAVSRHGLLPRAHLALPELNVPPLTLPENTA
                     LAALHYLKRQAGQKADWRLAVDGVRACTQEFWKHLPVMQKRSFLRHLQTYWDVHRHRM
                     APSIADYIKGLQGSGRLQVVAGRLEMMKLSADGRRVDVVLAHRGGTKFEPFSVDLVVN
                     CCGPQSTPRAIDCPFLTRLYERGMVVANDTNSGIKVDGLEVLDGAGHSSRNLYALGPL
                     LKGALLESVAVPEIRVQAQELARLICQRAGKN"
BASE COUNT          654 a          756 c          681 g          603 t
ORIGIN      
        1 ctagttaagt ctcgaaagtc cccgtcacca gcacctttca ggaaagtgac cttcgtcctt
       61 ttgtagatgc cgcgtaggca ccgagcaaca aataatttca catctagacc agcctgcgtc
      121 agaaatattt gtaaatacac gacagcaaac acaatagggt ctaaagacaa ctccttaatg
      181 atgttcttga gttaaatgag cactctcttg attttcgatg ccagtgcggt ctgagtgcat
      241 gctcgtgtac aagcttctct ggcgctccca gggcaaggcc gattggatgg aagaatgcat
      301 cgtgaccgtg gtggaatcag aaaaagatga tggcactgca gcttgtgaca ctatcgccac
      361 ctagatttag cctaaacttg gccgataaac cgccgggcgg tgccctgtaa ataagaaagt
      421 tagaccaaac cctgaaagca ccggtgaaaa gatgctaaat ttcacctgtc cggtgttcat
      481 taacacctac tttgaaattc gggggcgttc ggtttgaaaa aaggtctgct taatcaggtg
      541 gttgttcttg ctctggccgc cctggccccg gccttcagtg atgctggagc cctggcctgc
      601 accaggaccg tctgcttcgg caaagacgat atcgtcgtca cggctcgctc ccttgactgg
      661 atggaagaca tgcattccaa tctctgggcc tttccgcggg gcatgaaaag gaacggagca
      721 gccggcaaaa attccctgac ctgggtttcg aaatacggct ccatagtcac ctccggctac
      781 gatgcaggca cggccgacgg tctcaacgaa aagggactgg cggcaaatgt tctctacctg
      841 gtggagtcgg attacggcaa gggcgacggc ggtaaaccgg caatttccat cggtgcctgg
      901 gcccagtatg tgctcgataa ttttgccaca accaaagaag tagtagcagc tctatcaaaa
      961 gaaccctttc gtgtaattgc ccccaccctg ccaaacggtg tgccggccaa cttacacctg
     1021 gccgtctcgg accagtcggg agactccgcc atattcgaat acatcggcgg cactttgaaa
     1081 attcaccacg acaaaagcta tcaagtcatg accaattccc ccgacttcga tcagcaactg
     1141 gccttgaaca gctactggca gaaactgagc ggccagacct ttctacccgg cacccacaga
     1201 gcggcagacc gttttgccag agcctccttc tatataaaca gcatcaaaga atccaaagat
     1261 gaagacgagg cagtcgccca gtccttcagc gtcattcgca gtgtctcagt gccgctgggc
     1321 atagccacca gtggtcaacc gaacatttcg tcgacaatct ggagagttgt ggccgatcat
     1381 aaaaggctcg tttatttctt cgactcggcc atgagtccca atgtcttctg ggtgaagatg
     1441 gccgatcttg atctaaagga aggcgctccc gtgaagaaac ttaccctgac tgagggcaag
     1501 acatattccg gtaatgccgc cggcttcttt cagaagtcta agccctttga gtttttgccg
     1561 gcagaccagt aactgaaccg gcattttcag agcctagttt ttgccggctc tctggcagat
     1621 aagcctggcc agttcttggg cttgcactct gatttccggc acggccacac tttccagaag
     1681 cgctcccttg agcagggggc caagagcata gagattccgg ctggaatggc ctgccccatc
     1741 cagcacttcc agtccatcca ccttgatacc actattggta tcattagcca cgaccattcc
     1801 gcgctcgtaa aggcgcgtca ggaaagggca atctatggca cgcggcgtgc tctggggtcc
     1861 gcagcagtta acgacaagat ccaccgagaa aggttcaaat ttggttccgc cccggtgggc
     1921 aagcaccaca tctacacgcc ttccgtcagc gctcagtttc atcatttcaa ggcgcccggc
     1981 cactacctgc aagcgaccgc tcccttgcaa acctttgata tagtcggcaa tgctgggggc
     2041 catccggtgg cgatgcacat cccaataagt ttgcaggtgc cgcaggaagc tgcgcttttg
     2101 catgaccggc aagtgcttcc agaactcctg ggtacaagcg cgcactccat caacggcaag
     2161 gcgccagtct gccttctgtc cggcctgccg cttcaaatag tgcagggcag caagtgccgt
     2221 attctccggc agcgtcagag gcggtacgtt tagctcagga agagccagat gggcacgtgg
     2281 caatagacca tgacgagaaa cggcaacgat tcggcgaggg ctgctgcgag tctcttttgt
     2341 aagttcgagc actttatcca cagcggtcag accagtgccg accaagagaa tgtctgaacc
     2401 cgctgtttgc acctgagacc agcgctcccg gctccaggga tcatgaatat aatgcctatc
     2461 ctccagaggc agcttgtgaa gccaatttgg acgacgtccc ggtagattac ccagggcaag
     2521 cactacggta tcagcgataa tatcttcacc accggcaaga gagagtttat aaacatctcc
     2581 gcatctctcc atgtctctaa ccagatcttg cacatgggtg aaggaatggg caggttcaga
     2641 gctcttctcc agcacttcag ccaattgctc tggcttgtgc tcgttgacat ccaa
//
LOCUS       DAOQNZ010000466         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21292_length_2687_cov_4.158055, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000466 DAOQNZ010000000
VERSION     DAOQNZ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21292_length_2687_cov_4.158055"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKW73_09180"
     CDS             complement(<1..343)
                     /locus_tag="PKW73_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73500.1"
                     /translation="MTDNEKPPVGIGYPLAMLVKALAGEGEASETKVGKWRNVIWGIL
                     SGNLQVGSRTPVQGTPPWVTLEVVHGGFATGGYMAGGKLQPFELKKLSSVSQALEAST
                     DASTHALASLKK"
     gene            complement(393..464)
                     /locus_tag="PKW73_09185"
     tRNA            complement(393..464)
                     /locus_tag="PKW73_09185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(430..432),aa:Arg,seq:tcg)
     gene            603..>2687
                     /locus_tag="PKW73_09190"
     CDS             603..>2687
                     /locus_tag="PKW73_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HNA73501.1"
                     /translation="MKHPPSSSTTEPQPETIGGSVERVTFHSEESGFCVLRVKVKGHK
                     DLVTVTGSAASISPGEFVECHGHWFNDKTYGMQFKSQRLTVVPPSTLAGIEKYLGSGM
                     IKGIGPHFAKKLVKAFGEAVFEIIEETPERLLELPGIGSKRKEQVERGWSDQKAIREI
                     MVFLQSYGVGTSRAVRIYKTYGKEAIAKVSENPYRLALDIHGIGFKTADTIAQKLGIP
                     KDSLIRAQAGVRHVLQELSGEGHCACESNNLVEHAEKLLEIDAALAAQAIEHEVQEGN
                     LVEDFIEEKRVLYLTALHKAEVGVAASLMRIAAGSAAWSNIDIETALPWVEEQAKIKL
                     SESQKKAVALALTNKLLVITGGPGVGKTTLVNSILRIIAAKQARILLCAPTGRAAKRL
                     SESTSLTAKTIHRLLEFDPKIFGFKRNAENQLEADIVVIDESSMIDIVLMNQLLKAIP
                     DNAALLLVGDVDQLPSVGPGAVLADIIASEAVPVVRLTEIFRQAATSKIIVNAHRINQ
                     GQLPVKEAPPQDGKQAELSDFYFVAAETPEDISQKLMQIVAERIPKRFGLDAIKDVQV
                     LTPMNRGGLGSRSLNAELQKLLNPNSGPQLTKFGWNYGVGDKVLQIINNYDKEVFNGD
                     IGTISSIDMEEAELLIDFDGREVKYDASELDEVSLAYAASIHKSQGSEYPAVVIPLAT
                     QHYMMLERNLLYT"
BASE COUNT          700 a          742 c          695 g          550 t
ORIGIN      
        1 ctttctttaa agacgcgaga gcatgggttg atgcgtcagt ggaagcttcc agagcctgac
       61 tgaccgagga taatttcttc agttcgaagg gctgcaattt accgccggcc atgtagccgc
      121 cggtggcaaa tccaccgtga acaacttcca gggtcaccca gggcggtgta ccttgcaccg
      181 gcgtgcgaga gccaacctgc agattgccgc tgagaatacc ccagattaca ttgcgccact
      241 tgccgacctt agtttcggag gcttcaccct cgccagccag agccttcacc agcatagcca
      301 ggggataacc gatgccgacc ggcggttttt cattgtcagt cattgcttca ttgctcaccg
      361 aaaatgccgc cggctaatcc ggcagaaaac ttacctgaag gcaggaatcg aacctgcgcc
      421 ctaccggtcc gaagccggtc gctcaaccac agagcctctc cagggaatca aagccctaaa
      481 ctggagtcac cttcagcaat ctacctccac ctgaactcgg ttaaactcat aaaacccaat
      541 gacaagacac aagctccgat tagctcatat aatcggaagc ggcgtactta gaccggggaa
      601 ttatgaaaca cccgccatct tcatcgacca cagaacctca accggagaca atcggcggtt
      661 cggtggagcg tgtcactttt cacagcgaag agagcggctt ctgcgtgctg cgcgttaagg
      721 tcaaggggca caaagacctg gtgaccgtta ccggctccgc cgcctccatt tctcccggtg
      781 aatttgtaga gtgccacggg cactggttta acgacaaaac ctatggcatg cagttcaagt
      841 cgcagcgcct caccgtggta ccgcccagca ccctggccgg cattgagaaa tacctgggct
      901 cgggcatgat caaaggcatc ggcccgcatt ttgccaagaa actggtaaaa gcttttgggg
      961 aagcggtctt tgaaattatc gaggagacgc cggaacgcct cttggaatta ccgggaatcg
     1021 gcagtaaacg caaagaacag gtagagcgcg gctggtccga tcaaaaggcc attcgagaaa
     1081 tcatggtctt cctgcaatct tacggtgtgg gcacctccag agccgtgcgc atctacaaga
     1141 cttacggcaa ggaagccatt gccaaagtat cggagaatcc ctaccgactg gccctggata
     1201 ttcacggtat aggtttcaaa actgccgaca ccatcgccca gaaactaggt attcccaaag
     1261 attctctaat aagagcccag gccggagttc ggcatgtttt gcaggaactc tcaggtgagg
     1321 gtcactgcgc ctgcgagagc aacaatctag ttgagcatgc cgaaaaatta ctggagatag
     1381 atgccgcact ggcagcccag gccattgagc atgaagtgca agagggcaat cttgtagaag
     1441 attttatcga agagaaacga gtgctctatc taaccgcctt acacaaagcg gaagtaggcg
     1501 tagccgcaag cctcatgcgc attgccgcag gcagtgccgc ctggagcaat atcgatatag
     1561 aaacggcgct gccctgggtg gaagagcaag ccaagataaa gctttcggag tcacaaaaga
     1621 aagccgtagc ccttgccctc accaataagc tattggtgat aacaggcggg ccgggggtcg
     1681 gaaaaaccac tctcgtcaat agcattttgc gcattatcgc cgctaaacaa gcccgtattc
     1741 ttctttgcgc ccccaccggc cgtgccgcca aaagactctc ggaatcgact tctctaacag
     1801 ccaagacaat tcaccgcctg ctcgaatttg atcctaagat cttcggcttc aagcgcaacg
     1861 ccgaaaatca attggaagca gatatcgtcg tcattgacga atcgtccatg attgatatcg
     1921 tactcatgaa tcaactgctg aaagccattc ccgacaatgc cgccctgctt ctggtcggcg
     1981 atgtggacca gttgccttcg gtggggccgg gagcggtgct cgccgacata atcgcctcgg
     2041 aagctgtacc ggtggtgagg ctgacggaaa ttttcagaca ggccgccact tcgaagataa
     2101 tcgtcaacgc ccacagaatc aaccagggtc aattgcctgt caaagaagca ccgcctcaag
     2161 acggcaaaca agcggagctg tcggactttt attttgtggc ggcagaaacg ccggaagata
     2221 tcagtcagaa gctcatgcaa attgtggcag agcgtatacc aaagcgcttc gggctcgatg
     2281 ccatcaagga tgtgcaggta ctgacgccca tgaaccgggg cgggctgggc agccgctccc
     2341 tcaatgcaga attgcagaag cttctcaacc ccaatagcgg cccgcaactg actaagttcg
     2401 gctggaatta tggagtgggc gacaaagtct tgcaaatcat caacaactac gacaaagaag
     2461 tcttcaacgg cgacattggc accatatcca gtatcgatat ggaagaggcg gagcttttga
     2521 ttgactttga cggccgcgag gtcaagtatg acgcctccga actggacgag gtatcgcttg
     2581 cctatgccgc ctccattcat aaatctcaag gctcggaata tccggcggtt gtaataccgc
     2641 tggctacgca acactatatg atgctggagc gcaatctgct ctacacc
//
LOCUS       DAOQNZ010000467         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21330_length_2685_cov_2.834981, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000467 DAOQNZ010000000
VERSION     DAOQNZ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21330_length_2685_cov_2.834981"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKW73_09195"
     CDS             <1..424
                     /locus_tag="PKW73_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73502.1"
                     /translation="QSAQAQTQAAGLPAAALPSAADILQAPPMVSGDRLLWEIRPGIR
                     LVKSCLRWDPAIGVFRAQLEMLINGVWYPVIFYEVFPQGSVRHEYSPGGKRKSVRIDL
                     PPATVQELADNDLASKWLAYCRQYLTLISAAGGGTAPG"
     gene            743..>2685
                     /locus_tag="PKW73_09200"
     CDS             743..>2685
                     /locus_tag="PKW73_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73503.1"
                     /translation="MADPNKPNEQDQKVEKPQQKVEDQAAAVASNALRAEVFKPPAQA
                     DKGAVGEKTPGVAGSEKDLKDGALIVPVNIADARFKGLGQQAGHEGDGAKTPESAGKD
                     KAASDKDKAASDKDKAEPGTGDKAAVKQEPTASDSIMPDWMKKAGESVSTTAGELADS
                     ALQKASELKDAAVKKVGELADSVVQTASELPADAVNKMKELANSTVEVGNTVIAGVSS
                     AVLGSEAAAATLPQVDAKVGEQTSAGSLVTDKSAGIAAPPLESGAVAVTDANTGMVKP
                     VIQDAQPIQPVVEKPLSQEEKELQEAKKDAGSASNQSGSVVVGAVSQPVKSGSSSAPT
                     GNDLSMPPLSGDKTVPTQVSDTSLPPLTGAGAEQNKVLKSDLPPLSGNVAEPVTAIKS
                     DLPPVAKTVGETAAHEVDLSPKKFSVQSPTIAETVPEKPVPTVAKIEQAQTNFEPSSM
                     ATVGLPPLSDSAKVTPSPVTADQSKVDAAPGATDQSKLVAAPGATDQSKVVPETKPEE
                     PGLIDKTIDAVKSGYKAVVEWFSGDNKIKYGNDEFKKNSDTGTWTGPDGRTYKSATLD
                     DKTGRLVLEGTNGIKEVRTSDGAREVTSKDASGKETVADFGADGKLNALTVGGTSYQF
                     DAQTKSWSDADGKPVKDVSYEPST"
BASE COUNT          619 a          715 c          776 g          575 t
ORIGIN      
        1 gcagtcggct caggcccaga cgcaggccgc tggcttgccg gcagccgccc tgcccagtgc
       61 tgccgatatt ctgcaggccc ctcccatggt gtccggtgat cgattgctct gggagatcag
      121 accgggtatc aggctggtca agtcttgctt acgctgggac ccggctatcg gcgtcttcag
      181 ggctcagttg gaaatgctca tcaacggtgt ctggtacccg gttatcttct acgaagtttt
      241 cccgcaaggt tcggtcaggc acgaatattc gcccggcggc aagcgcaaat cggtgcgcat
      301 agatctgccg cctgccaccg tgcaagaact ggctgataac gacctggcct ctaagtggct
      361 ggcttactgc cggcagtacc tcactctcat atctgctgcc ggtgggggga ctgctccagg
      421 ctgaagtttg ccttgacctc tgcgtttaat attaattttg gggttccggt ctgatttgac
      481 ggtccggatt gggccttttg ttgtccagac aacagtcagc gtttccaatt tgcatatata
      541 ttagtgttct ctaaggagtc gggcttcttc cccgcaccac ggaggtctga cggctcttgg
      601 caggcgatga gaaactggcg tcccttttac tagattagaa cttactttca gccattttct
      661 ttcgtttaaa agaggcctcg gatcgtgaat attggtaagt ggatgaccag ttggggtcat
      721 ccattcgaca gaggtctacg gtttggccga tccgaacaaa cccaatgagc aggaccagaa
      781 ggtcgagaag cctcagcaga aggttgagga tcaggctgcc gcagtggcta gtaacgcatt
      841 gcgggcggaa gttttcaagc cgccggctca ggcggacaaa ggtgcggtcg gggagaagac
      901 tcccggtgta gccggtagcg agaaagattt gaaagacggg gccttgattg tgcccgtcaa
      961 cattgccgat gcccgcttca aagggctggg ccagcaggcc ggtcatgaag gtgatggtgc
     1021 taagactcca gaatctgcgg gcaaagacaa agctgcttcc gacaaagaca aagctgcttc
     1081 cgacaaagac aaagcggaac cgggaactgg tgacaaggcg gcggtcaagc aagagccgac
     1141 ggcgtccgac agcatcatgc ccgattggat gaaaaaggct ggcgaatcgg tctctacaac
     1201 ggctggtgaa ctggccgata gtgcattgca gaaagccagt gaattgaaag atgccgccgt
     1261 caagaaggtc ggtgagttag ctgattcagt tgtccagacc gcctctgaac tgcctgcgga
     1321 tgccgtcaac aagatgaaag aattggcgaa ttctaccgtc gaggtgggca acaccgtcat
     1381 agcaggagtt tccagcgcgg tgcttggctc ggaggccgcc gccgccaccc tcccgcaggt
     1441 tgatgccaag gttggtgaac agacttctgc cggttccctc gtcactgata agtcagcggg
     1501 aatcgctgct cctccgcttg aaagtggggc tgtcgccgtt accgatgcca ataccggcat
     1561 ggtcaagccg gtcatccagg acgcgcagcc gatccagccg gtggtggaaa agcctctctc
     1621 tcaagaagag aaggaattgc aagaggctaa gaaggacgcc ggttcggctt ccaatcaatc
     1681 cggctctgtc gttgtgggag cagtctcaca gccggtcaag tctgggtcta gttctgcgcc
     1741 taccggaaat gacctttcca tgccgcccct atccggtgac aagacggtgc cgactcaggt
     1801 ttccgataca agtttgccgc ctctgaccgg tgctggcgct gagcagaata aggttctgaa
     1861 atctgatttg ccgccccttt caggcaatgt tgccgagcct gttaccgcca tcaagagcga
     1921 tttgccgccg gtggctaaga ccgtcggcga aacagccgca cacgaagtcg atctcagtcc
     1981 caagaaattc tcggtgcagt ctccgacgat agctgaaaca gtgcccgaaa agccggtacc
     2041 gactgttgcc aaaattgaac aggcacaaac aaactttgag ccatccagta tggccacggt
     2101 tggtttgcct cctttgtccg attcagccaa agttactccg tccccggtga cagccgatca
     2161 atctaaggtt gacgctgcgc ctggggcgac cgatcaatct aagcttgtcg ctgcgcctgg
     2221 ggcaaccgat caatctaagg tcgtgccgga gacgaaacct gaagagccag gtttgataga
     2281 caaaactatc gatgccgtaa agtccggtta taaggcggtt gttgagtggt tctcaggcga
     2341 caacaagatc aagtatggga atgacgagtt caaaaagaac tccgatacag gcacttggac
     2401 tggacctgac ggcagaactt acaagagtgc cactcttgac gacaagaccg gcaggctggt
     2461 gcttgagggc accaatggta tcaaggaagt gcgtaccagc gacggtgcca gggaagtcac
     2521 ctccaaggat gcttccggca aggaaaccgt tgcagatttt ggcgccgatg gcaaactcaa
     2581 cgctctaacg gttggcggta catcctatca attcgatgca cagaccaagt cctggtccga
     2641 tgctgatggc aagcccgtta aagacgtcag ctatgagcct tccac
//
LOCUS       DAOQNZ010000468         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21547_length_2670_cov_3.426769, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000468 DAOQNZ010000000
VERSION     DAOQNZ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21547_length_2670_cov_3.426769"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1065)
                     /locus_tag="PKW73_09205"
     CDS             complement(121..1065)
                     /locus_tag="PKW73_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73504.1"
                     /translation="MSKQVKDQSDNSPSAKGLVAKIGLSQSDPTVETVPEMFVKFSLK
                     NPGKVSVNPALTQTELVIDGKPFEQSAQLFGNGPRDARFSSLPAGETIDFSYAIGYYF
                     RKAGEHTLVWRGPSFEPVQIKFWVAPLSGQWKEVARCPCATVAVERALYRKDGSKDFF
                     VHVRIENLLKRPIGFLAGDRYQVFYPNQWADSELPRRQVISEMRRLQKELSTAERQKL
                     LALFTSGPKPVGSNRRVKILPGNSSDYYVSFNAGGRDRVEAITLPYLIVVMDGVIDFS
                     DGKELYRASREMNDSNHGEVALLTPVKLKDVPADALIF"
     gene            1401..>2670
                     /locus_tag="PKW73_09210"
     CDS             1401..>2670
                     /locus_tag="PKW73_09210"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA73505.1"
                     /translation="MSSTTMPVLIKKATSRVRKPHHLEGATALLFACLLALTPAAAQT
                     ARPEAVHSDKPIQDKWALIVGISNFKNSNIPRLNYAAKDAADFYQFLIKDAGFAPDHV
                     RILLDEKASQRRVMSELGSKFLARVAKRDDLVVLFFSTHGSPAQMDLRGDNYLVAHDS
                     DPDDLFASGIEMQRVLDATNKRVLTDRVLLILDACHSGALKPGAKGMKRAANFDAEAL
                     AQGSGQMVICSSSTDEQSFESRRYKNGVFTKHLIDSMHGGDKPKELASAFSELKEKVS
                     AEVLEDRQMRQTPVLNSQWDGGALVLAAAAVNPQQLPAIVKEELEPDSSETLKIAVQS
                     KARSSQESPSSSKENSRGNRAVAANSLSTTSDTSEKLVLSSDYFKEEGDPKQLLRAYN
                     ATIRENPREAEMFYKRACIYIQLKQWIPAHN"
BASE COUNT          719 a          712 c          675 g          564 t
ORIGIN      
        1 accgggtgcg gcaaaagcca gaaattgcag ggcgcagagg gcaatatact taaatttcac
       61 gacgctcaac tgttaaggaa ggaaaggctt aattgtatca aatgggattt actcaccaag
      121 ctagaatatc aaagcatcag cgggcacatc cttcagcttc accggagtca aaagagccac
      181 ttccccgtgg ttcgagtcat tcatctcgcg gctggctcta tacaattcct ttccgtcact
      241 aaaatctatg acaccatcca tgactacaat cagataaggc aatgttattg cttccacccg
      301 atcgcgaccg ccggcattga aggagacata gtaatcagaa ctatttccag gcaggatttt
      361 cacccggcgg ttgcttccga caggctttgg tccagaggta aagagtgcaa gcaatttctg
      421 ccgctcagca gtgctaagtt ccttttgcag gcgacgcatc tcggaaatca cctgccgcct
      481 gggaagttcg gaatcggccc actgattagg atagaaaacc tgatagcggt cgccggccaa
      541 aaagccaatc ggccgcttca ataaattttc aatgcgcacg tgtacgaaga aatctttact
      601 gccgtccttt cggtaaaggg cacgctccac tgccactgtg gcacagggac aacgggccac
      661 ctctttccac tgcccgctca agggcgccac ccaaaactta atctgcaccg gctcaaaact
      721 gggacctcgc cagaccaggg tgtgctcacc agccttacgg aagtagtaac cgatggcata
      781 actgaaatcg atggtttctc ccgcaggcaa actggaaaaa cgggcatcgc gcgggccgtt
      841 tccaaagagc tgggcggact gctcgaaggg cttaccatcg atcaccagct ctgtttgcgt
      901 caaagccgga ttgacgctaa ccttgcccgg gttcttcaga gaaaacttga caaacatttc
      961 aggcacggtt tcaactgtgg gatcgctctg ggagagccct atttttgcaa ccagaccttt
     1021 ggcagaaggg ctgttatcag attggtcttt cacctgctta gacatcgtct ctaatctccc
     1081 gggactaccg ggaaccatag gcgggcaaaa tagaaaagag ccgatcaata ggagagcagc
     1141 gctgcatttc agggcaaaga gcggcgtggt tccaggtaga tggtcgagtt tctttctcat
     1201 gcccccatga tgtcagatcc cctcatgaaa gtccccggtg aaaaccctga cagcaatccg
     1261 gcagcctgct aatattcgca gacaaggaag ccctgtgtaa gataggtaag cctgctctgc
     1321 ctgtcttaca ggaagaccag attaaatcgg agcattcgcc aggcttataa tagaacaggt
     1381 taagtctgcg aggtaggctc atgtcatcga caacaatgcc ggtgttgata aaaaaagcta
     1441 caagtcgggt aaggaaaccg caccaccttg aaggcgctac agcccttctc tttgcatgcc
     1501 tgcttgccct tacaccggca gcggctcaga cagctagacc ggaagctgtt cattcagata
     1561 aaccgattca ggacaaatgg gctctcattg tcggcatcag taatttcaag aactcaaata
     1621 ttccccgtct caattatgcg gccaaggatg cagctgactt ttaccagttt ctcatcaaag
     1681 acgccggctt tgcccccgac catgtgcgca tactcctgga cgagaaggct tcccagcgaa
     1741 gagtcatgtc cgaattgggc agtaagtttc tggcccgcgt agccaagcgg gacgaccttg
     1801 tggtgctctt cttttccacc catggcagcc ccgcccaaat ggatttgagg ggtgacaact
     1861 acctggtggc ccacgactct gatccagacg acctgtttgc atcgggaata gaaatgcaga
     1921 gggttttaga tgctacaaac aagagagtct tgacggaccg cgtgctgcta attctagacg
     1981 cctgtcatag tggtgcgctc aaaccgggag ccaagggcat gaaacgcgcg gccaatttcg
     2041 acgccgaggc cttagcccag gggtccggac agatggtgat ttgttcttcc agcaccgatg
     2101 agcagtcttt tgagtcaaga cgatacaaga acggagtttt cacaaaacat ctcatagaca
     2161 gcatgcatgg cggcgacaaa ccgaaagagc tggcctctgc cttttccgaa ctgaaagaga
     2221 aggtgagcgc ggaggtactg gaagacagac agatgcgcca gaccccggta ctcaacagcc
     2281 agtgggacgg cggtgccctg gtcctggcag cggctgccgt caatccccaa caactgccgg
     2341 ccattgtcaa agaagaactt gagccggaca gttcggaaac tctcaagatt gcagtgcaaa
     2401 gcaaagcaag atcgtcgcag gaatctccct ccagttcaaa ggagaactct agagggaata
     2461 gagcagtagc ggcaaatagt cttagcacca ccagcgacac tagtgaaaag ctagtgctaa
     2521 gcagcgacta tttcaaggaa gaaggcgacc ccaagcagct actaagagca tacaatgcca
     2581 ccattcgtga aaatccgcgc gaagccgaaa tgttctacaa gagggcttgc atctacatac
     2641 agctcaagca gtggattcca gcccataatg
//
LOCUS       DAOQNZ010000469         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21550_length_2670_cov_2.978585, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000469 DAOQNZ010000000
VERSION     DAOQNZ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21550_length_2670_cov_2.978585"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1673)
                     /locus_tag="PKW73_09215"
     CDS             complement(87..1673)
                     /locus_tag="PKW73_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNA73506.1"
                     /translation="MDIMLLGGFGIGFVALIFVCYFFANLYQKCGPNEAMLITGFTPG
                     GPQIVRGGGKIVMPLIHQKNILSLEVMTIEVHSQAPMITKNGIPIYVDGVAQVKVRGD
                     EEAIATAAERFLNKNQDEIATVAHETLVGHLRAILGTMTVEELIQSFDSFACKVQEVS
                     VADLAKMGLTVDSFTIKEIRDTVGYLEALGKKQTAEAKRNAAIGEAEATKETEIARAN
                     ASRDAQIAQAQAAEEGAKAKLAADTRVAESSKNFQVSQAQYQAEVSRTKAASDMMYDI
                     VKAQSQQKLIEETQKIKIIEAQKEVELQQVEVTKRQVALEVEITKPAEAEQLRIRLMA
                     QAEQEKRRMLAEADALSAKLAAQGQAEAMKLKAMAEADAKRAIGMAEAEAERAKGLSE
                     AQVTAAKGQAEADAMAKKAEAYKQYNDAAIANMIVEKLPELVQAAASPLAKVGNITLL
                     SSGGESTGITKISADVMGVAAQSLTMIKGLTGVDLTESLKNKISASTEKGNSGGNSNP
                     GNNSGVPQATVKLPPTQNNG"
     gene            complement(2007..>2670)
                     /locus_tag="PKW73_09220"
     CDS             complement(2007..>2670)
                     /locus_tag="PKW73_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNA73507.1"
                     /translation="FAEHGDSTDPGHLHLHQHGDCGHGHGGHAQITSTMAGHGNATMD
                     GHGHGAGHGGSNHGHNEMEDLKSTGNTVVASVKTSTYFQILGILNPTSISVFTGFVGL
                     TGLVLMKCLPFLGLLTLVPAAVVGLVSVIVMRNVFSYLTAKLNVSNTVKSTDAIGHVA
                     EVSASIGQGGTGEVTYVIGTTRFNAAAKSASSSETIKRGSKVLIVEREGHLVYVEPYQ
                     ES"
BASE COUNT          490 a          702 c          710 g          768 t
ORIGIN      
        1 caccaacaac tatgattctt gtttccttac tgttgatggc tgcctcaatt cagcgtgctt
       61 gttgctgtcc ttccgttttc gtccgctcag ccgttattct gagtgggagg caacttcacg
      121 gtagcttgtg ggacgccgga gttgtttcca ggattggaat tgcctccgga gttgcctttt
      181 tctgtcgaag cgctgatttt gtttttcaga gattcggtca gatctacccc cgttaagcct
      241 ttgatcatgg tcaggctctg cgcggcgaca cccatcacgt ctgctgagat tttggtgatg
      301 ccggttgatt ctccaccgct ggaaaggagg gtgatattgc ccactttggc tagaggagaa
      361 gcagcggctt gcaccagttc aggcagtttt tcaactatca tgttggcgat ggctgcgtcg
      421 ttgtattgct tgtaggcctc tgctttcttg gccatggcat cggcttctgc ctgtcctttg
      481 gccgcagtga cctgagcttc ggatagacct ttggctcttt ccgcttccgc ttccgccata
      541 ccgatggcgc gttttgcgtc ggcttccgcc atggctttca gtttcatggc ttccgcttga
      601 ccctgggcag ccagcttggc agatagagca tcagcttcgg caagcattct gcgtttttcc
      661 tgctcggcct gagccatcaa tctgattctc agctgttcgg cttcggccgg tttggtgatt
      721 tccacttcca gggctacctg gcgcttggtc acttctacct gttgcagttc cacctctttc
      781 tgcgcttcga tgattttgat cttctgcgtt tcttcgatga gcttctgctg cgattgtgct
      841 ttcacgatgt cgtacatcat gtctgatgca gctttcgtgc gagaaacctc tgcttgatac
      901 tgagcttgcg aaacctggaa gtttttggag gattccgcta cgcgagtatc tgcggcgagc
      961 ttggcttttg ccccttcttc agcggcctgt gcttgagcga tttgagcgtc tctggaagca
     1021 ttggcgcggg cgatttctgt ttctttggtg gcttccgctt caccgatggc ggcgttcctt
     1081 ttggcttcag cagtttgctt tttaccaagg gcttctagat agcccacagt gtctcttatc
     1141 tctttgatcg tgaatgaatc aaccgtcagt cccatcttgg ccagatcggc cacggaaact
     1201 tcctgcactt tgcaggcgaa ggaatcgaag ctctggatca gttcttccac ggtcatagtg
     1261 cccagaatgg cccgcaggtg accgacgaga gtttcgtggg caactgtggc tatctcgtcc
     1321 tggtttttat tgaggaagcg ttctgcggct gtagcgatag cttcttcgtc accacgcact
     1381 ttcacctgcg ccacaccgtc tacgtaaatc gggattccat tcttagtgat cattggtgct
     1441 tgcgagtgca cttcaatagt catgacctcc agagaaagga tgtttttctg gtgaatcaag
     1501 ggcatgacta tcttgccgcc acctctgacg atctggggcc cgccgggagt gaaaccggtg
     1561 atgagcattg cttcgttggg accgcatttt tgatataggt tggcgaagaa gtagcagacg
     1621 aaaatcaggg ctacaaaccc aattccaaag ccacccaaga gcatgatgtc cattttttga
     1681 acctcctgtc ggtttggacc cgaccgattt aatttaatct ctagctgcct tattccctaa
     1741 taattgaatt ctctcggcgg ctgtctgata ttgttgcttg acttagttga acctatacta
     1801 aaccgaatat ggtggcgtcg tcgctattaa tgcacattcc tttataatct tgacaaccct
     1861 tcggcacggg catttttctg actttcaggc tggtctgcgg ctgagaaacc gctcccctta
     1921 tctcaggcga ttggagaggc tttgagctgc ccccttttct gtttcctcag tgcaggaccg
     1981 gtcggctttc tcaccggcgg ggctgtttat gattcctgat agggctcaac gtagaccagg
     2041 tgcccttctc tttcaacgat cagcactttg gagccccgtt tgatggtttc gctggaggag
     2101 gcgcttttgg cggcagcgtt gaaacgggtc gtgccgataa cataagtcac ctcgccggta
     2161 ccgccctggc cgatagatgc gcttacttca gctacatgac cgatggcatc ggttgatttg
     2221 acggtattgg aaacgttcaa cttggcggtc aggtaggaaa agacattgcg catgacgatg
     2281 accgaaacca gtcccaccac agctgccggc accagggtga gcagacccag aaagggcagg
     2341 catttcatta gaaccagccc ggtcaacccg acgaagcctg tgaataccga tatggaagta
     2401 gggttgagga taccgaggat ttgaaagtaa gtggaggttt taaccgaggc taccacggtg
     2461 ttgccggtgg acttcaggtc ttccatctcg ttatgaccgt gattgctgcc gccgtgacct
     2521 gcgccatgac cgtgcccatc catggttgcg ttaccatgac cggccatggt tgacgtaatt
     2581 tgcgcatggc cgccgtgacc gtgaccacag tccccgtgct ggtgcaagtg caggtgcccc
     2641 gggtcggtgc tgtctccgtg ctcggcgaag
//
LOCUS       DAOQNZ010000470         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21577_length_2668_cov_3.916954, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000470 DAOQNZ010000000
VERSION     DAOQNZ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21577_length_2668_cov_3.916954"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PKW73_09225"
     CDS             complement(<1..613)
                     /locus_tag="PKW73_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73508.1"
                     /translation="MVWLFASARLPVLAIEKTDPIDTNRPSFCQSGIVVPRGTLQLEN
                     GSQYQHLQHGGDYFDIPETEVRIGATRRLELQMFVPQYVLVHGPSQSFEGASGLNEVG
                     LKTQLIDREHLSASLVTAVNIPSGMRRISGSVAQPVLRLPYSIHLGKRWAVCGMQSLI
                     INGPGGEVQWQPFAMVTRSLSPTSSAFAEYAGFFTQNSHRPSFQ"
     gene            803..1174
                     /locus_tag="PKW73_09230"
     CDS             803..1174
                     /locus_tag="PKW73_09230"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNA73509.1"
                     /translation="MSPSRKPENSPTCCPIQVLLPDVQPLTDEEAGDLEDLFKILAND
                     TRLKLLHHLARVEEMCVCDLSTAMEMNVQAISNQLQKLSDRKIVATRRSGNMIYYRLI
                     DNCLIEILKQAQCLLIDSKTK"
     gene            1501..2217
                     /locus_tag="PKW73_09235"
     CDS             1501..2217
                     /locus_tag="PKW73_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNA73510.1"
                     /translation="MHTQYQDYKDGDLTCEAYIATDENKKGKRPAVLVSHAWGGQSDF
                     ERGKAEKLAQLGYVGIAIDLYGKGNRGNSMEENAKLMQPFMDDRAMLRKRILAALSFA
                     KSLPEVDGDKIGAIGFCFGGLCVLDLARSAAAGVQGVVSFHGLFHAPNIGEQKPITAK
                     ALILHGYDDPMATPEQMVALAAEMTKASADWQIHAYGNTVHAFTNPEANMPENGIVYC
                     EKADKRSWQAMVNFLAEALA"
     gene            complement(2214..>2668)
                     /locus_tag="PKW73_09240"
     CDS             complement(2214..>2668)
                     /locus_tag="PKW73_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73511.1"
                     /translation="LAGPGAPDTYLCQGNMGQALFGLGEFAQAEVLMKEALAGVEQHE
                     GKDSIGAATFCDNLAVLYCRQGEPAKAESMQVRALGIYTRKVGRENPDALICMGNLAL
                     TYERQGKLKEARKTAGEAFEIAVKVLGEAHPVTERLRNILSGLATPAP"
BASE COUNT          692 a          725 c          672 g          579 t
ORIGIN      
        1 cctgaaagga cggtctatga gaattctgag taaagaagcc ggcatattcc gcaaaagcgg
       61 aggaagtggg agagaggctg cgggtcacca tggcaaaagg ctgccactgc acttcaccgc
      121 caggaccatt gatgatcaga gactgcatac cgcaaacggc ccagcgctta cccaggtgta
      181 tgctgtaagg cagtctcagc acaggctgag ctacgctgcc actgatgcgc ctcataccag
      241 agggtatatt caccgccgtt accagtgagg cagagagatg ctcccggtct atgagttgcg
      301 tcttgagccc aacttcattc aaaccgctgg ctccttcaaa agattgagag gggccatgaa
      361 cgagcacgta ctgaggcacg aacatctgta attccaggcg ccgagtggca ccgattctca
      421 cctcggtctc aggtatgtcg aaataatcgc cgccatgctg caagtgttga tactggctac
      481 cattctccag ttgcagggta cccctgggta ccacgatgcc ggactggcaa aagcttgggc
      541 gatttgtatc tatggggtcg gtcttttcga tagcaaggac aggcaacctg gctgatgcaa
      601 aaagccagac caaaagacat aagaacttta cgcgcccgtc attgtccgct cttttaccta
      661 gcctcaaagc atgaacctta gaaacccttc tccagttaag gataggacat cagccccggg
      721 gcgtttggat taattatgaa ttttttggag aaagcattac actgcttcgt gtaatagtgt
      781 aatataaaac tcggaggtag tcatgtcccc cagtcgaaag cccgaaaaca gcccaacctg
      841 ctgcccgatc caggtattgt tgccggacgt gcaacctctc accgacgaag aagccggtga
      901 tctcgaagat cttttcaaaa tcctggccaa cgacaccagg ttgaaactgc tgcaccatct
      961 ggcccgggtg gaagaaatgt gtgtctgcga tctgtccaca gccatggaaa tgaatgtgca
     1021 ggcaatttcc aatcaactgc aaaagctttc cgaccgcaaa attgtcgcca ccaggcgcag
     1081 tggcaatatg atctactacc gactgatcga caattgtttg attgaaatac tcaaacaagc
     1141 tcaatgcttg ctaatagaca gcaagaccaa gtaaataccc aggagggatc gaaacatgga
     1201 aaagacctgt tgtgaatgtt gcgacaaaaa agactgcaaa ccgtgccagg gctgccagtg
     1261 ctgctgctgc tgttgcaaag tatacgaaga ggggaaagct tgcccctgca ccccagagaa
     1321 accctgtaaa tagaaaaaga aatggctttg agaaggcgca gtttcatacc tgcgccttct
     1381 cccgtcccta aacaagtgtt gcgcttgcct taattgcccc ccggggctct ggacaaatcc
     1441 tttgccaagc cctgaaaatt catgcctgta tctactttct aagactcagg ctggtaaaaa
     1501 atgcatacgc aatatcaaga ctacaaagac ggcgatctga cttgcgaagc ctatatcgcc
     1561 acagatgaga acaagaaagg caaacgcccc gcagttctgg taagccatgc ctggggcgga
     1621 caaagcgact ttgaacgggg caaggcggaa aagcttgctc agcttggcta tgtgggcatt
     1681 gccatcgacc tttacggcaa gggcaatcgt ggcaattcca tggaggaaaa tgccaaactc
     1741 atgcagccct tcatggacga ccgcgccatg ctgagaaagc gcattctggc ggccctctcc
     1801 tttgctaaga gcctgccgga agtagatggc gacaaaatag gtgccattgg cttttgcttc
     1861 ggtggccttt gcgtgctcga tctagctaga agtgcggctg ccggcgtgca aggcgtggtg
     1921 agcttccatg gtcttttcca cgcccccaat atcggcgaac agaagccgat tacagcgaaa
     1981 gccttgattt tgcacggcta tgacgacccc atggccacac cggagcagat ggtagccctg
     2041 gccgccgaaa tgacaaaggc ttccgccgac tggcagattc acgcttacgg caatactgtc
     2101 catgccttca ccaatccgga ggccaatatg ccggaaaacg gcatagtcta ttgcgagaaa
     2161 gccgacaaac gctcctggca ggccatggtc aacttcctgg ccgaagccct ggcctagggc
     2221 gcaggtgtag caagccctga aagtatattg cgcaagcgct ctgtgaccgg atgcgcttcg
     2281 ccaagaactt tcactgcaat ttcaaaagct tcacctgctg ttttccgagc ttccttaagt
     2341 ttgccttgcc gttcataagt tagagccagg ttgcccatac agatcagggc gtccggattt
     2401 tcacgcccaa ctttcctggt gtaaataccc agtgctctaa cctgcatgga ctctgccttg
     2461 gcaggttcac cctgcctgca atagagcacg gccagattgt cgcaaaaggt agctgctcca
     2521 atactgtctt ttccctcgtg ctgctcgaca ccggcaaggg cttctttcat caagacttca
     2581 gcctgcgcaa actctcccag tccaaagagc gcctgtccca tattgccctg acagagataa
     2641 gtatcgggag caccgggacc agccagcc
//
LOCUS       DAOQNZ010000471         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21586_length_2667_cov_4.415773, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000471 DAOQNZ010000000
VERSION     DAOQNZ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21586_length_2667_cov_4.415773"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKW73_09245"
     CDS             <1..288
                     /locus_tag="PKW73_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73512.1"
                     /translation="PLIEHHPSIPTAPERGNGSMESMDDAQSGAVGPEPPFRSNYFLP
                     VENTPKNSGAPAQFGPAGPGGPAGVGGPAGPAGPAGPGGPSGPSGAGSIGG"
     gene            372..1265
                     /locus_tag="PKW73_09250"
     CDS             372..1265
                     /locus_tag="PKW73_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73513.1"
                     /translation="MGVDSAMHVSRSAASFRHTESAAFSRKFGRFLSVACVALALGAT
                     SLFSPLQNVPVFAAEKAPVSELAPDLERPVVIYLHSLSSNNGEPFCFPGGKKGFIYHA
                     FKINSPKLEMKSLEYDYGKVFTGDYADLVNEVEACVKEFNIKTRRLILAGTSIGGYAI
                     FPLYAKLPPQTRDLVMGAIAVACPENLEICAKKTRAENMLNIYKPYSEGLPNMSLSRM
                     LANASVPPVVVYMRYHEDLTVPYACSSKLLPFLKKKGAVCREITLEGKHGIPAQINYE
                     VAYQQFKSLVRKSPEEADAVK"
     gene            1457..>2667
                     /locus_tag="PKW73_09255"
     CDS             1457..>2667
                     /locus_tag="PKW73_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73514.1"
                     /translation="MAKAKEVSEEKQQAQPVDSPQLSQEANALGAKTVSQPTEDVSGK
                     VTSQALKTELAKFSPDSALVLDHLRSKSPEELKTIAGNYKSETGRNLGDDLAKSFSGS
                     ERVLVTQALQGNLDDTTRVRANVLALGELSGNSNKNVEKDLRDTFATMNKEQIDKVAA
                     EYKERFGRVLVSDLQNNTSISADSKQALSVYMKGTDNRSSEDVQRLAGLALASKNIDM
                     MRETFRDASPEARHQFLGADGEKKIKAAFGSKEAAIALDYVNLGRLDVATAVKHNSSF
                     AGDNEAAIYFAAASMSKQQKDDYAQGRLNAERDAAGLTPGEQKQVEFYRKVSEAFKDP
                     LSDREMLICEDKILSPGGKSTVSKIAEMGGFIGASGKDVNAALKAMPPAELERLQNDP
                     QFRKNLDSAIN"
BASE COUNT          618 a          669 c          752 g          628 t
ORIGIN      
        1 cccctgattg agcatcatcc atcgattcca actgcccctg agcgaggcaa cgggtctatg
       61 gaatcgatgg atgatgctca atcaggggca gttggtcctg agccgccctt ccggtcgaat
      121 tactttttgc cagtcgagaa tactccgaaa aactcagggg caccggcgca gtttggtccc
      181 gctggaccgg gcggacctgc aggcgtcggt ggtcccgctg gaccagccgg tccggccggc
      241 cctggtggtc cttccggccc ttcgggcgcc ggtagcattg gcggctgaaa acttgcccgt
      301 cctgccggcc tgccgatctg atgcaatggc ggcatctgtt aaactagggt ttttcggagg
      361 tctttcggtc catgggtgtg gattctgcaa tgcatgtttc taggtcagcc gcttcgttta
      421 gacatactga atcagcggct tttagccgta agttcggtcg tttcctctcc gttgcctgtg
      481 tggcgctggc tctgggagcg acgtcgctct tttctccatt gcaaaatgtg cccgtcttcg
      541 ccgcagaaaa agccccggta agtgagctgg ctcccgatct ggagcgcccc gtcgttattt
      601 acctgcacag ccttagcagc aacaacggtg agcctttctg cttccctggc ggcaagaagg
      661 gctttattta ccacgccttt aagataaaca gccccaaact cgagatgaag tcgcttgaat
      721 acgattacgg caaggttttt accggtgact atgctgatct tgtaaacgaa gttgaagcct
      781 gtgtgaaaga attcaacatc aagaccaggc gcttgattct agccggcacc tccattggtg
      841 gctatgccat cttcccgctc tatgccaagt tgccgcccca gacccgagat ctggtcatgg
      901 gagctattgc tgtggcctgt cctgagaatc tggaaatctg cgccaagaag acccgggctg
      961 aaaacatgct caatatttat aaaccctatt ccgaagggtt gccgaatatg agtctttccc
     1021 gcatgctcgc caatgccagt gtgccgcctg tagttgtata catgcgttat cacgaagatc
     1081 tgaccgtgcc ttacgcttgc agtagtaaac tgctgccctt tctgaagaag aagggagcgg
     1141 tttgccgaga aatcacgctg gaaggtaagc atggtattcc agcccagatc aattacgaag
     1201 tggcctatca gcagtttaag tcgcttgtgc gcaaatcccc cgaggaagcc gacgcggtta
     1261 aatagtgatg gcttagactg cggttttgcc cttattgctt ctggaagttc tcgaaaatcc
     1321 tgtaattcat tcggtgaaaa ggcgggagag cttctctgtg cttgaagttt gagcctttgg
     1381 ggaatatgaa tctggtaggt ctggaatagt tcgcgaatcg ggctgaaaat ccgatggtat
     1441 ctgagcggta agtgtcatgg caaaggcgaa ggaagttagt gaggagaagc agcaggctca
     1501 gcctgtggat agtcctcagt tatctcagga agccaatgct ctgggcgcca agacggtcag
     1561 tcagcccacc gaagatgtct cgggcaaggt tacttctcag gcgctgaaaa cggagctggc
     1621 caagttctct ccggacagcg ccctggtgct tgatcacctg cgcagcaagt ctcctgagga
     1681 attaaagact atcgccggta actacaagtc tgagactggt cgcaacttgg gcgatgatct
     1741 ggctaagagc ttttccggtt cagagcgagt tctggtcact caggccttgc agggcaatct
     1801 ggacgacact acccgggtgc gggcaaatgt gttggcgctg ggcgaactga gcggcaacag
     1861 caataaaaac gtcgagaagg atctgcgtga cacctttgcc acgatgaaca aggagcagat
     1921 tgataaagtt gcggcggagt acaaagagcg ctttggaaga gtgcttgtct ccgatttgca
     1981 gaataacacc agtatcagcg ctgatagtaa gcaggcgctt tctgtttaca tgaagggtac
     2041 cgataatcga agcagcgaag atgttcaaag actggccggg ttagcgctgg cttcgaagaa
     2101 catcgacatg atgcgcgaaa cttttcgcga tgcttctcct gaagctcggc accagttctt
     2161 gggcgcagac ggcgaaaaga agatcaaagc cgcttttggt tcaaaggaag ctgctatagc
     2221 actcgattat gtgaacttgg gtcgtcttga tgttgccacg gcagttaagc ataattccag
     2281 ttttgccgga gacaacgagg ccgctatcta ttttgccgct gccagcatgt cgaagcagca
     2341 gaaggatgat tacgcccagg gcagactgaa tgccgagcgc gatgcagccg gtcttacccc
     2401 cggcgagcaa aagcaggtag agttttaccg caaagtcagc gaagccttca aagatccgct
     2461 ttccgaccgg gaaatgctca tctgtgaaga caagattctt tctccgggcg gcaagtcgac
     2521 ggtgtctaag attgctgaaa tgggcggttt catcggtgcc agcggcaaag atgtgaatgc
     2581 cgcgttgaaa gcgatgccgc cggcagagct tgagcggctc cagaatgacc cgcagtttcg
     2641 taaaaacctc gactcggcaa taaatga
//
LOCUS       DAOQNZ010000472         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21597_length_2667_cov_2.967458, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000472 DAOQNZ010000000
VERSION     DAOQNZ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21597_length_2667_cov_2.967458"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1021)
                     /locus_tag="PKW73_09260"
     CDS             complement(2..1021)
                     /locus_tag="PKW73_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73515.1"
                     /translation="MTRLFGLMLAATLFSGLVGVNMAVFGAKPAGVSQPRAGKVVSRA
                     LVFPGQVSLGKLYFYVDAEPGGLPWHCDVVFAKPGSGLGDARGVRTFKLPEHSRIYLF
                     PSYHLLEHPDLFKLLDPNSFDGLSFGKTSLMVPVENCFSQLVHLKGLRRLELEGAELS
                     DQQIVPLKGLVNLESLSLPMNTIDGTCFNQMLSLSKLRELDLSLNKISPDGFRAISKF
                     KNLEVLYLDKSGVDDAALEHLTTLPKLKHIGLGQSKVTARGLSLIKKMKSIEVVDLEG
                     APLAVKDFGILRGSGVNSLSLSGKQFSPAQIKQIGAMLPGVQFTIAPADSANSDHKML
                     FAPLH"
     gene            complement(1578..2531)
                     /locus_tag="PKW73_09265"
     CDS             complement(1578..2531)
                     /locus_tag="PKW73_09265"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA73516.1"
                     /translation="MRKIALEEHFNGPGFEKYLSAVAGEFDPAVLAAVEKLLPEFDEK
                     RLQVMDACDIEIAVLSQTAPGVQQETDTGRATKAACSVNDFLAAQIARHPKRYRGFAC
                     VALQDPVQAARELERCVKDLGFVGALVNGHTCGTYLDHPSFQPFWEKLVDLQVPLYLH
                     PGLLYDEPHMFSGRRELSGATWSWTCETATHALRLVFGGVFEKFPQAKVILGHMGETL
                     PFELWRLDSRAQTTSAGRAMKHPPSEYIRKNIVVTTSGVCANGPLLCSLLELGEDSVM
                     FSTDFPYEDALVAARFIEEAPISREQREKVCFRTAERLLRL"
BASE COUNT          716 a          714 c          648 g          589 t
ORIGIN      
        1 actagtgcaa aggagcaaaa agcatcttat ggtcggagtt ggcgctgtca gcgggcgcaa
       61 tagtgaactg aacgcctggc agcattgccc ctatttgctt gatctgagca ggtgaaaact
      121 gcttgcctga aagactcaaa ctgttcaccc cggagccccg taaaatgccg aaatctttta
      181 cggcgagggg cgccccctcg agatctacca cctcgatgct cttcattttc ttaatcagag
      241 acaagccccg cgccgtcact ttcgactggc caagaccaat atgctttaac ttcggcaagg
      301 tggtcaggtg ctccagagca gcatcgtcga cgccggattt atcgagatag agtacctcca
      361 gattcttgaa cttagagata gcccgaaagc catccgggct gattttattc aaagatagat
      421 caagttcccg caacttcgac aaactcagca tttgattgaa gcaagtacca tcaatcgtgt
      481 tcattggcag gctcagagac tcaagattaa cgaggccctt gaggggcaca atttgctggt
      541 cagaaagttc ggctccttcc aactcaaggc gtcgaagccc cttcaaatgc accaactgtg
      601 agaaacagtt ctcgaccggc accatgagag aggttttgcc aaaggagagc ccatcaaaac
      661 tattgggatc aagcaatttg aacaggtccg ggtgctccag gagatgataa gaagggaaaa
      721 gatagatcct tgagtgttcg ggcaacttga aggttctgac tccgcgtgca tcgccaagcc
      781 cgctgccggg cttggcaaag actacgtcac agtgccaggg taaaccgcct ggttctgcat
      841 cgacatagaa atagagtttg cccagtgaca cttgtccggg aaaaaccagc gcccgactca
      901 ccactttgcc ggctcgaggt tgtgaaacac ctgccggctt ggcgccgaaa acagccatgt
      961 tcacccccac caggccgcta aacagagtcg ccgccaacat aagaccaaac agtctagtca
     1021 tgggcagacc atgaattgag gacaattgcc attgacacca gaaaccacat ctaccgggga
     1081 gcttacttca tagtaaccgg gaacctcccg gaaatcacta cacttatatg cctcctttag
     1141 gaggatagac aacgccttaa cttaactgga ctgtttcaag taatcattcc aattgatgat
     1201 gccgcaggca aaggcgagag ttattataaa aacgttcgaa gcgaggtcat tcacacaaaa
     1261 gagcagaagc accgaacaga acttcaaacg aaatagcaga ccacctgaac ggactcaatc
     1321 ccgccgggaa ctgatttcaa cctcatccaa gtacactctt gttcaatctt cttgtcctct
     1381 ttgaaatgtg gtgttttgat cgacaaattt tagaaacggc cgtcccaagc ataaccctct
     1441 taagggctta atttcgggga ctgccgtttc tttcttttta caatagctga gtgccgcagc
     1501 ggactaacag gcgcttctgc ctgcatcgaa aagcagaact aaagccgcaa gtatagaccg
     1561 gcaattgcga acgccgtcta aagccgtaag agtcgctcgg cagtcctgaa gcaaaccttc
     1621 tcacgttgct ccctacttat cggggcttct tcaatgaatc gcgcggccac cagtgcatct
     1681 tcatagggaa agtctgtcga gaacataacg ctgtcttcac ctagctccag cagagaacaa
     1741 agcaaaggac cgttggcaca gacaccggat gtggtgacga caatattctt gcggatatat
     1801 tccgaaggcg gatgcttcat ggctctgccg gcgctggtag tctgcgctct gctgtcaaga
     1861 cgccagagtt cgaagggcag cgtttcaccc atatgtccga gaattacttt tgcctgcgga
     1921 aatttctcaa agacgcctcc gaagacgagc ctcagcgcat gggtagccgt ttcgcatgtc
     1981 cagctccagg tggcaccgga gagttctctg cgccccgaga acatatgggg ctcgtcatag
     2041 agcaggcctg gatgaagata taaaggcacc tgcaaatcca ccagcttctc ccagaagggc
     2101 tgaaagctgg gatggtccag ataagttcca caggtgtgtc catttaccag ggcgccgaca
     2161 aaacccaggt ctttcacaca acgctctaac tcgcgagcgg cctggacggg atcttgcaaa
     2221 gccacacagg caaagcctcg atagcgcttc ggatggcgag cgatctgagc agcaagaaag
     2281 tcattgacgc tgcaggcggc tttcgtcgcc ctgccagtgt cggtttcctg ctgcacccct
     2341 ggagcggtct gcgagagaac agcaatttca atatcgcaag catccatgac ttgcaggcgt
     2401 ttctcatcga attcaggcaa gagtttttca acggcagcca gaacggccgg atcaaactct
     2461 ccggcaactg cactgagata cttctcaaag ccggggccat tgaaatgctc ttccagagca
     2521 atttttctca tgagtatttc tgtcctttcc ggaattgcct gtcagcgaga atacaagtat
     2581 tgatacgata gcagaaaact cttccataga atttttaaag acgcactacc agaatgccct
     2641 ggtccaaaga atcggagcga cggttcc
//
LOCUS       DAOQNZ010000473         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21611_length_2666_cov_3.517043, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000473 DAOQNZ010000000
VERSION     DAOQNZ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21611_length_2666_cov_3.517043"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /gene="queC"
                     /locus_tag="PKW73_09270"
     CDS             <1..646
                     /gene="queC"
                     /locus_tag="PKW73_09270"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNA73517.1"
                     /translation="WAKARYNKVFAVTFDYAQRHRREIESARKIAEMAAVQHEVIDLG
                     PIFKGLSPLTDHSLAVDHFSDCNTLPGSEPGGLAQTFVPGRNILFFTVAANRAYVLGC
                     DTIVVGVAQQDYGGYPDCREDFVVKLEAALASGLERPLKIETPLMHLTKKATVELAAS
                     LEGCMEALSFSTTCYEGFYPPCGECHSCLLRHKGFSEAGLEDPLLGRALAEARR"
     gene            698..1201
                     /locus_tag="PKW73_09275"
     CDS             698..1201
                     /locus_tag="PKW73_09275"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNA73518.1"
                     /translation="MGEASSLSSGKQDSALTVSAVRRLQFCAGHRVYKHESKCANLHG
                     HNYVLFLHATAEADDASHQDGLDSLGRVVDFGKLKQLFNPWLEDNWDHGFILFKEDEE
                     AVAALSRFPEQKLFLLDTNPTAENMARFILKDLAPRLLEGSGVKLTKVVLWETENCFA
                     EVEYARS"
     gene            1188..1889
                     /locus_tag="PKW73_09280"
     CDS             1188..1889
                     /locus_tag="PKW73_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNA73519.1"
                     /translation="MLGANPIRKQEVGASFDGGELWVQEVFYTLQGEGPFSGHPAVFV
                     RLSGCNLPCYFCDTDFESSTWRPSLNELMSKVQAALGKSCKLVVLTGGEPFRQNIQVL
                     VKALLALDLTVQIETNGTLYVDLPWCENLHVVCSPKTAALNNDLVPHISAYKYVIKAG
                     EVEEEDGLPGGSTRTSGRPDFIARPEGEAPVYVMPMDEQDEKRNAANQAACIESALKF
                     GYRLTLQTHKIIGVR"
     gene            complement(1873..>2666)
                     /locus_tag="PKW73_09285"
     CDS             complement(1873..>2666)
                     /locus_tag="PKW73_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73520.1"
                     /translation="SQFEAEPTAPSDKPDQAPNDSPAQTRVIYSKNRASRMELITTLD
                     SRNILNAISVSWPPYQERTSEMPAPPGENKPLPPSIDKSGQSQADCGKHLQSAKTLIA
                     KRKFKEARKQLLYSILVIEEHPDPAVVRRAYEDTRECYIKLYSSWNRPDVVDYFENTF
                     YKKLRSDAISVDSGRRTDFPGFTDYKNELCRVTGNTKVGYRIEFPADYAGLEIQELPP
                     DSPHFSEIKSYFGDVPPSGRNIDPPPAIWLDDACFAITEPATAPQ"
BASE COUNT          550 a          642 c          772 g          702 t
ORIGIN      
        1 ctgggcaaaa gcccgatata acaaagtttt cgccgtcaca ttcgattacg cccagcggca
       61 tcgtcgtgag attgaaagcg cgcgcaaaat tgcagaaatg gctgctgttc agcatgaggt
      121 catagatctc ggaccgatct tcaaaggtct cagtccgctc accgaccaca gtctggctgt
      181 ggatcatttc tcggattgca ataccctgcc aggctcggag ccaggcgggc tggctcaaac
      241 ttttgtgccg gggcgcaata ttttgttctt tacggtggcg gctaaccggg cttacgtact
      301 gggttgcgac accattgtgg tgggtgtggc tcagcaagat tacggcggtt atcccgactg
      361 ccgcgaagat tttgtcgtca agttggaagc ggctctggcg agcggtcttg agagaccctt
      421 gaagattgag acaccgctta tgcacctgac caaaaaggcg actgtggaac tggctgcttc
      481 tctggagggc tgcatggagg ctctttcctt cagtaccacc tgctatgaag ggttctatcc
      541 gccctgcggt gaatgtcatt cttgcctctt gcgtcataaa gggttctcgg aagccgggtt
      601 ggaagatccg ctattgggac gagccctggc tgaagccagg cgttgaggtg aagggctgat
      661 gggtgcgggg cgtggtagcg caggtggtgc aatggaaatg ggggaagcct caagtctttc
      721 ctccggcaag caagattctg ctttgacggt ttcggctgtg cgcaggttgc aattctgcgc
      781 cggccataga gtttataagc acgagagtaa gtgtgctaat ttgcacggac acaattatgt
      841 gctgtttttg catgccacag cagaagccga cgatgcttct catcaggatg ggctcgattc
      901 tctggggcgg gttgtggatt tcggcaagtt gaagcagctc tttaatccct ggttggaaga
      961 caactgggac catggtttca ttcttttcaa agaggatgag gaagccgtgg cagccctctc
     1021 tcgtttcccc gagcagaagc tcttcctgct cgataccaat cctaccgccg agaacatggc
     1081 tcgcttcatt ttgaaagatc tggctccccg actgcttgaa ggtagcggcg tcaagctgac
     1141 caaggtggtg ctctgggaaa ccgagaattg ctttgccgag gtagagtatg ctcggagcta
     1201 atcccatccg caagcaagaa gtcggtgcct cttttgacgg tggtgagctc tgggtgcagg
     1261 aggtgtttta taccctgcaa ggggaaggcc cattctcggg gcatccggct gtgtttgtgc
     1321 gtctttccgg ctgtaatttg ccctgctatt tttgtgacac tgattttgaa tcatctacct
     1381 ggcgaccttc tttgaatgaa ctaatgtcca aagttcaagc tgccctgggg aagagttgta
     1441 agctggtggt tttaacaggc ggtgagcctt ttcggcagaa catccaggtc ctggtgaaag
     1501 ctttgctggc tcttgatctg actgttcaga tcgaaaccaa cggcactctc tatgtcgatc
     1561 tgccctggtg cgagaatttg catgtggttt gcagccccaa gactgccgcc ctcaataacg
     1621 atcttgtgcc tcatatctct gcctacaagt atgtaatcaa agccggcgag gttgaagaag
     1681 aagacggctt gccgggcggt tctacacgca ctagcggcag accggatttt atcgctcgtc
     1741 cggagggaga ggcccctgtc tatgtgatgc ctatggacga gcaggacgag aaaagaaacg
     1801 ctgccaatca ggctgcctgc attgaatccg cgcttaagtt cggctacagg ctaacgctcc
     1861 agacccataa gattattggg gtgcggtagc gggctctgtg atggcgaagc aagcgtcgtc
     1921 cagccaaatc gccggaggtg gatcgatgtt tctgccggac ggcggaacgt ctccgaagta
     1981 tgactttatt tcagaaaaat gaggcgaatc aggggggagc tcctgaatct ccagaccggc
     2041 atagtctgct ggaaattcaa tccggtagcc gacctttgtg ttgccggtta cgcggcacag
     2101 ttcatttttg tagtctgtaa aacctgggaa gtctgttctt cgtccgctgt ctactgaaat
     2161 ggcatcgctt ctgagcttct tgtaaaaggt gttttcgaaa tagtccacta catccgggcg
     2221 attccaactg ctatagagct ttatgtagca ttctcttgtg tcttcgtagg ctcttcttac
     2281 cacggctgga tcgggatgct cttcaatgac cagtatcgag taaagcagtt gtttgcgcgc
     2341 ttccttgaat ttacgctttg cgatgagagt ctttgctgac tgaaggtgct tcccacagtc
     2401 ggcctggctc tgaccggact tgtctatcga cggagggagc ggcttgtttt ccccaggcgg
     2461 tgccggcatt tcactggttc gctcctggta gggcggccag cttacggaga tggcgtttaa
     2521 gatgttccgc gaatctaaag tagtgattag ttccatgcgc gaggctctgt ttttgctgta
     2581 gataactcgt gtctgcgccg gagagtcatt tggagcttga tccggcttgt ctgaaggtgc
     2641 agttggctct gcttcgaact gcgaag
//
LOCUS       DAOQNZ010000474         2665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21627_length_2665_cov_3.683525, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000474 DAOQNZ010000000
VERSION     DAOQNZ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21627_length_2665_cov_3.683525"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..>2665
                     /gene="ileS"
                     /locus_tag="PKW73_09290"
     CDS             299..>2665
                     /gene="ileS"
                     /locus_tag="PKW73_09290"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNA73521.1"
                     /translation="MKEKSTYSVNLPQTAFPMKANSAVRELEIQKVWDDTGVYEASLK
                     RREAAPKYVLHDGPPYLSSSKIHIGTALNKVLKDIVTKYKGLKGFYSPYVPGYDSHGL
                     PIENAVLKDVKGGRAALTPVELRQRCRAFALSNLKGQEANFRRLGVWGHWQSPYITLD
                     QHFEAKQVRVFGKMAGKGYLYKGLKSVSWCPNCETALAEAEVEYADHTSNSIYVKFAV
                     DAASKSKLPAAVQDKEVAFVIWTTTPWTLPANLGVALHPDFVYEFLQVEDESHVLVVA
                     ESLKNAFLSAAGKAEKAVQVLATALGKELELLECRHPFMDRLSRVINGDHVTAEAGTG
                     CVHTAPGHGPEDFEIGKVYQLGVLSPVDSRGIFTEEAGIFAGQRFDKANPLVVEHLKE
                     KGVLLQHSTFSHSYPHCWRCKKPLIFRATEQWFASVEGFRKRALECIDAVEWLPASGR
                     NRIFNMVENRSDWCISRQRAWGVPIPVFYCKGCHKPLMSEESIEAVAKVFDAEGSDSW
                     WEREPGYFLKDIGCGHCGGKEFDRETDIMDVWFDSGVTHAAVLDERPELRGTPCELYL
                     EGSDQHRGWFQSSLLTSVAVSDRAPYKTVLTHGFVVDESGRKMSKSVGNVVEPDEVIK
                     QYGADVLRLWVASVNYTDDIPIGKNMLAQLADVYRRLRNTARFLLGNLHDFDPATDMV
                     KKESLSSLDRFVLHRLNEVVAEVTRDFDRFEFFKYYQLLQNFCGVDLSAFYFDIVKDR
                     LYTCHPKDESRRAVQTVLTHTLHTLVRLLAPVTPHLAEDIWQHMSEPLR"
BASE COUNT          578 a          718 c          744 g          625 t
ORIGIN      
        1 ggcagggctt tcctttggtg agaaatagac ttgctaccgc tggtgatacc agagcggctg
       61 ccgaaccgtc acagggatga aaagtggttt gtatttacgt tccaagtata tgtttacgct
      121 tcttgcagat ttattgacga ccggggttcc ccggctctat ccttaactgc ggcgaggaga
      181 ttgtgtcagt gcggaatatt attcggacta ttattattga atgctaatca agctggcgta
      241 gtcgtagaaa gacaccgccg gcgccaagcg ctggcgtttt tgttatttgg agtagagcat
      301 gaaagagaag tcgacctata gtgtgaacct gccccaaacg gcatttccca tgaaagccaa
      361 tagcgccgtt cgggaactgg agattcagaa ggtctgggac gacaccggtg tatacgaagc
      421 cagcttgaag cgccgggaag cggcgcccaa gtatgtactc catgatggac cgccttacct
      481 gtcttcgagc aagattcaca ttggtacggc tctcaacaag gtgctcaagg acatcgtcac
      541 caagtacaag ggcttgaagg gcttctactc gccctatgtg ccgggctatg acagccatgg
      601 tctgcccatt gaaaacgcgg ttttgaaaga cgttaaaggc ggtcgcgccg ctttgacgcc
      661 ggtggaactg agacagcgct gccgggcctt tgccctcagt aacttgaaag gacaggaagc
      721 caacttccgc cgtctgggcg tctggggcca ctggcagagc ccttatatca ccctggatca
      781 gcacttcgag gccaagcagg tgcgggtctt cggcaaaatg gcaggcaaag gttacctcta
      841 taaaggtttg aagtccgtct cctggtgtcc taattgcgag accgccctgg ctgaagccga
      901 ggtggagtac gccgaccata cttccaactc catttacgtc aagtttgcgg tggatgcagc
      961 atccaagtct aagttaccgg ctgccgtgca agataaggaa gtggccttcg tcatctggac
     1021 cacaactccc tggactcttc ctgccaacct cggtgtggct ttgcaccccg acttcgtcta
     1081 tgaattcctg caggtggaag acgagtctca cgtactggtg gtggcggaga gcctcaagaa
     1141 tgctttcctg agcgccgccg gcaaggccga gaaagctgtg caggttctgg ctacggctct
     1201 tggaaaagag ctggagttgc tcgaatgtcg ccatcctttc atggaccgcc tttcaagggt
     1261 gataaacggt gatcacgtta ctgccgaagc cggtacgggc tgcgtacaca cggcgcccgg
     1321 tcatggtccc gaggactttg aaattggtaa ggtctaccaa cttggtgtac tttctcctgt
     1381 tgattcccgc ggcatcttca ccgaggaagc cggtattttc gccggtcaac gcttcgacaa
     1441 agccaatcca cttgtggtcg agcacttgaa agagaaaggc gtgctcttac agcattccac
     1501 cttctcccat tcttatccgc actgttggcg gtgcaaaaag cccctcatct ttagagccac
     1561 cgaacagtgg ttcgcttccg ttgagggctt cagaaagaga gccctggagt gtatcgacgc
     1621 cgttgaatgg ttgcctgctt caggccgcaa ccgcatcttc aacatggtgg agaaccgttc
     1681 cgactggtgt atcagccggc agcgggcctg gggcgttccc attcctgtct tctattgcaa
     1741 gggctgccat aagccgctta tgagcgaaga aagtatcgaa gccgtggcca aggtcttcga
     1801 cgccgaggga tcggattcct ggtgggaaag agagcctggc tatttcttga aagatattgg
     1861 ttgcggacac tgcggcggca aagagttcga cagagagacc gacatcatgg atgtctggtt
     1921 cgattccggc gtcacccatg cggctgtgct cgacgaaaga ccggagttgc gcggcacgcc
     1981 ttgcgagctt tatctggaag gttccgatca gcaccgcggc tggttccagt cctccctttt
     2041 gaccagcgtg gctgtttccg accgcgctcc ctacaagacc gtgcttaccc acggtttcgt
     2101 ggtagacgaa agcggccgga aaatgtccaa gtcggtgggc aatgtggtgg agcccgacga
     2161 agtaattaag cagtacggag ccgatgtgtt gcggctctgg gtggcttccg tcaactacac
     2221 cgacgatatt cccatcggca aaaatatgct ggcccagctg gccgatgttt atcgccgctt
     2281 gcgcaacacg gcgcgcttct tgctcggtaa cttgcacgac ttcgatccgg ccacagacat
     2341 ggtcaagaaa gagagcctct cttctctgga tcgttttgtc ttgcaccgcc tcaatgaagt
     2401 ggtagccgag gtgaccagag acttcgatcg ctttgagttc ttcaagtatt atcaactgct
     2461 ccagaatttc tgcggcgtcg atctttccgc cttctacttc gatattgtca aagacaggct
     2521 ctatacctgt catcccaaag atgagtcacg ccgggccgtg cagaccgtgc tcacccacac
     2581 cctgcatact ctggtgcgcc tcctggcgcc tgtgacgccg cacctggccg aagacatctg
     2641 gcagcatatg agcgagcctt tgaga
//
LOCUS       DAOQNZ010000475         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21722_length_2659_cov_3.679724, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000475 DAOQNZ010000000
VERSION     DAOQNZ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21722_length_2659_cov_3.679724"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKW73_09295"
     CDS             <1..496
                     /locus_tag="PKW73_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73522.1"
                     /translation="MGMVAPNQGTYMAQNFAASMLSQLNGAMDKKQLERMSLNQAELI
                     MLYNMVRGTADKLVDNYRCYKKNILHMSRATTDLEELRNMAGMAAKSPAQELEMQYTL
                     RKAQRDIDGLVDDVHRYRQNLIDMAGNDAVSKLDNQVKEEIQKLEDTSSLAGKAKTAD
                     GTNM"
     gene            586..1578
                     /gene="sppA"
                     /locus_tag="PKW73_09300"
     CDS             586..1578
                     /gene="sppA"
                     /locus_tag="PKW73_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNA73523.1"
                     /translation="MRINQRWFVWIILTLCVLAIPVAVLTQGLASAPAAKDGNGSGLM
                     TKFKDRIQVVKLSGMIIDKADSGLLSSSAGSATETLKDLRKAIKNDKVKGVLLRVNSP
                     GGTVPTSQEIYEAVKELKKKNKIVVVSMGDMAASGGYYVSCAADKIVANPGTLTGSIG
                     VIMNLMNFKALADKVGIEPEVVKSGLFKDIASPYHKMTKEERQILTDLIMDSYDQFTQ
                     VVAEGRALPIEEVKRIADGRIYSGRQAKAIKLVDELGTYSDALALLQKLCKERFKNSA
                     DLPVEDVSNDSFLSTLLESAGAEAPPLSGKMELSSYVPVELSPALTRQPLWLYR"
     gene            1593..2444
                     /locus_tag="PKW73_09305"
     CDS             1593..2444
                     /locus_tag="PKW73_09305"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNA73524.1"
                     /translation="MSEAEKPRDEMPEAELEKLPDPEKRIVDSKPFELELAFRQVLRE
                     PEGKVETNTNKSVQLPAEEEAQTQVQALPEPGEGQKKSQYENSFNLFADIFYGVLVAP
                     RQTMLILSDQTKYPCTAGNIAQSGFFVIMVLSMTGWLRFKADSIQASVVSGLMFTLSG
                     ALNWFFMAAVLYYLSIFMRGRLKLGNALIATAWSFLPFIFFAPVLCFRTLLGNFFPLL
                     AMMPALWFLFLLFVTFQASLRTSIPKLSLILIVVPPLFAFVYIFWLVVAFGSLFIQIL
                     PRLLPAA"
     gene            2453..>2659
                     /locus_tag="PKW73_09310"
     CDS             2453..>2659
                     /locus_tag="PKW73_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73525.1"
                     /translation="MEKSETSIVAAKPLVIAIVGPTCTGKTRLAIELGQKLKTLAGKT
                     AEVLACDSRTVYRHMDIGTAKPSLA"
BASE COUNT          668 a          731 c          681 g          579 t
ORIGIN      
        1 catgggcatg gtggcaccca accagggcac ctatatggcc cagaactttg ccgccagcat
       61 gctcagccaa ttgaacggcg ccatggacaa gaaacaactg gaacgcatgt ccttgaatca
      121 agccgagctg atcatgctct ataacatggt gcgcggcaca gccgacaaac ttgtggacaa
      181 ttaccgttgc tacaagaaaa acattttgca catgagccgg gccacaaccg acctggaaga
      241 actgcgcaat atggccggca tggccgccaa gtcgccggcc caggaattgg aaatgcaata
      301 caccctgcgc aaagcccagc gggacatcga cggtctcgtg gatgacgtgc accgctaccg
      361 gcagaatctc atcgacatgg ccggcaacga cgcagtttcc aagcttgaca atcaagtcaa
      421 ggaagaaatt caaaaactgg aagataccag cagcctggcc ggcaaagcca aaacggcaga
      481 cggcactaat atgtaactgc acatattaag ccgatacaca atccatacag aacctcgccc
      541 tatcagctaa tatttgtagt ttagctttag ccgaggtcaa aactggtgcg catcaatcaa
      601 cgctggttcg tctggatcat cctcactctc tgtgtccttg ccattccggt ggcggtgctg
      661 acccagggtc tggccagtgc accggctgcc aaggacggaa acggctccgg actgatgacc
      721 aagtttaagg accgcatcca agtggtcaag ttgtccggca tgatcatcga caaggccgac
      781 tcggggttgc tttcatcttc agccggctcg gccaccgaaa ccttgaaaga cttgcgcaaa
      841 gccattaaga acgacaaagt caaaggggta cttttaaggg tcaattctcc cggcggtacc
      901 gtaccgactt cccaggaaat ttatgaagcc gtcaaagagc tcaagaagaa gaacaaaatt
      961 gtagttgtct ccatgggcga catggcggcc tcaggcggct actatgtttc ctgtgccgcc
     1021 gataaaattg tggccaatcc cggcacattg acgggcagta tcggcgtgat catgaacttg
     1081 atgaatttca aagctctggc cgacaaggtc ggcattgagc cggaagtggt caagtcggga
     1141 ctgttcaaag acatcgcctc tccctatcac aagatgacca aagaagagag gcagattctc
     1201 accgacttga ttatggactc ctacgatcag ttcactcagg tggtagccga aggacgggcc
     1261 ctgcccatcg aagaagtgaa acgcattgcc gacggtcgca tttatagcgg tcgtcaggcc
     1321 aaagccatca aactggtaga cgaactcggc acctacagcg atgccctggc gcttctgcaa
     1381 aagctttgca aagagcgctt caagaactcc gccgacttgc cggtagaaga tgtgagcaat
     1441 gacagcttcc tgtccacctt actggagagc gccggagcgg aagcgccgcc cctttccggc
     1501 aagatggaat tgagttccta tgtgccggtg gaactgtcgc cggctctgac cagacaacca
     1561 ctctggcttt atcgctaaag gcaaaggtac caatgagcga agcagagaag cccagagacg
     1621 aaatgccgga agccgaactg gaaaaattgc cggacccgga aaaacgtatt gttgattcaa
     1681 agccctttga actggagctt gccttccggc aagtcttgag agaaccggaa gggaaagtag
     1741 aaaccaatac aaataagagt gtgcaattac cagcagagga agaggcgcaa acgcaggtcc
     1801 aagcgctccc tgaaccaggg gaagggcaaa agaaaagcca gtacgagaac agcttcaacc
     1861 tctttgccga catattctac ggcgttcttg tggcaccaag acaaaccatg ctcatcctca
     1921 gcgaccagac caagtatccg tgcacggctg gaaacatagc tcagtccgga ttcttcgtca
     1981 ttatggttct ctccatgacc ggctggttgc gctttaaggc cgacagcata caggcctctg
     2041 tggtcagcgg tctcatgttc actctatcgg gcgcgctcaa ctggttcttc atggctgcgg
     2101 tgctctacta cctgagtatc ttcatgcgcg gtcgactgaa actgggaaat gcgctcatag
     2161 ccacggcctg gtccttccta ccctttattt tctttgcacc ggtactgtgt tttcgcacgc
     2221 ttctgggtaa tttctttccg cttctggcca tgatgccggc tctctggttt ctcttcctgc
     2281 tctttgtcac cttccaggcc tcgctgcgca catccattcc caagttgagt ctcattctca
     2341 tcgttgtgcc gccgctcttc gcctttgtct acattttctg gctggtggtg gcattcggtt
     2401 ctctattcat acagattttg ccccgcttgc tgccggctgc ttaggtcaga ttttggaaaa
     2461 gagcgaaact tccattgtcg cggcaaagcc cctcgtgatt gccatagtgg gtcccacttg
     2521 cacaggcaag accagactgg ccatcgagct tggccaaaaa cttaagacac ttgccggtaa
     2581 aacggcggaa gtgctggcct gcgacagtcg cacggtttat cgccatatgg atataggcac
     2641 ggccaagccg agtttggcg
//
LOCUS       DAOQNZ010000476         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21766_length_2657_cov_3.418524, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000476 DAOQNZ010000000
VERSION     DAOQNZ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21766_length_2657_cov_3.418524"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PKW73_09315"
     CDS             complement(<1..471)
                     /locus_tag="PKW73_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73526.1"
                     /translation="MWWKQFLVMPVALSFLLGGSSPQASKDDCLASLSDKDLLAHRLV
                     LRSEVNPQSLYKRVWNLIKDEYYDSKYNNQSWDRWEHRYDGKLRTLDDAHKAVETMVA
                     SLGDRYTRFLDHDAFDDEKSQIDAKLCGIGVQIGLDKSSKRVIVITPIEDTPASD"
     gene            909..1712
                     /locus_tag="PKW73_09320"
     CDS             909..1712
                     /locus_tag="PKW73_09320"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNA73527.1"
                     /translation="MADEVLADIGIFGGSGFYKLFDEYEEKMVETPYGAPAAPVAIGK
                     MGGKRVAFLPRHGEKHQFPPHKVPYRANVYAMKKLGVSRIISPCAAGSLQTDVKPGEF
                     VVCDQFVDRTSGRTDTFYDGPIATHVSAAELYCPEMRALAVAAGEKSGIKMHPKGTVV
                     VIQGPRFSSKAESRWFTSMGWEVINMTQYPEAFLARELEMCVVNVSLITDYDSGLVGN
                     VEPVSHSEVVKVFGQNISKLQTLLKNLIGTIPDGRAKCDCANTLKLARV"
     gene            1727..1921
                     /locus_tag="PKW73_09325"
     CDS             1727..1921
                     /locus_tag="PKW73_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73528.1"
                     /translation="MWRNFTINHVVALLALVLSIASYLVLRRYHFPDKICVGGAILCI
                     LVVAIFWSCVLLGTEEDDEE"
     gene            1953..>2657
                     /locus_tag="PKW73_09330"
     CDS             1953..>2657
                     /locus_tag="PKW73_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNA73529.1"
                     /translation="MFKRTSNLCFSLCSSLAFLALAGTAGAQAQPAADAGKASPITIK
                     KPLIPVTKDYTLKNGLRLIVSEDHSAPVASLAIVYDVGARDEQKGRSGFAHLFEHMMF
                     QGSENVGKVEHFKYIENVGGSLNASTHADFTNYFEKVPSNQIEMCLWLESDRMRSLKV
                     TPDNFKNQLDTVKEEKRMRVDNQPYAPSSVRLEELAFDNWGNGHPTIGYFEDLEASSV
                     KDVKQFFDTYYAPNNAT"
BASE COUNT          663 a          765 c          642 g          587 t
ORIGIN      
        1 atcggaggcc ggcgtatctt caatgggagt aatgacaata acgcgcttgc tggacttatc
       61 gagaccaatt tgcacaccga tgccgcaaag tttggcatca atctgagact tctcatcgtc
      121 gaaggcatcg tggtcgagaa agcgagtata gcgatcgcca aggctggcta ccatggtctc
      181 cacggccttg tgagcatcgt cgagggttct cagcttgccg tcatagcgat gctcccagcg
      241 gtcccaactc tggttgttgt acttgctgtc gtaatattcg tccttaatca ggttccagac
      301 ccgtttgtaa agggactggg gattgacttc gctgcgcagc accaggcgat gggcgagcag
      361 gtctttgtcg gaaagggaag ccaggcaatc atcctttgaa gcttgcggag aggaccctcc
      421 cagtaagaaa gatagagcca ccggcataac caaaaattgt ttccaccaca tgatgcgagt
      481 tcctgcccct gaatctgtat atccgtctga cctatgccgc cggcaccaaa accgaatgaa
      541 tgcgcgcgct gtgaatctct tatacccacc aataccaggc tttacgctct cctggcaaac
      601 atttttccag gcgaattcct gatttcaagc cagggtaaag cctgaatgca ctggaatcaa
      661 tccaggaccg atcaactgaa tgcaagcttt gatgccgctt tcgctcattt ccgacagcaa
      721 gaagcagacc ctatgggcgc cccgccctaa tggcgccgca cggacttcgg tggggaaaat
      781 cccctacaaa agcaaaactg ccggtaaatg ttaggatatg cttttcgacg gcggcgtttt
      841 ccctttgcaa caaaggaaaa ctctgccgtt caggctacac caacaccaat cgcgataaga
      901 ggtacacctt ggcagacgaa gttcttgcag atatcggcat ttttggaggc tccggcttct
      961 acaaactctt cgacgagtac gaagagaaaa tggtggagac accctacgga gcaccggcag
     1021 ccccggtagc tatcggcaaa atgggcggca agcgcgttgc cttcctgccc aggcacggtg
     1081 aaaagcatca atttccgccc cataaagtgc cgtaccgggc taatgtctat gctatgaaaa
     1141 agctcggcgt cagccgcata atctccccct gcgcagccgg ttccttgcaa accgatgtca
     1201 aaccaggtga atttgtggtt tgcgaccagt ttgtagaccg cacctccggt cgcaccgaca
     1261 ctttctatga cggtcccatc gccacccatg tctcggcagc cgagctctat tgcccggaaa
     1321 tgcgggccct ggcagtcgcc gccggcgaaa agtcaggcat caagatgcac ccgaagggca
     1381 ccgtggtagt aatccagggt ccccgtttct cctccaaagc cgaatcccgt tggtttacct
     1441 ccatgggctg ggaagttatt aacatgaccc agtatccgga agccttcctg gccagagaac
     1501 tggagatgtg cgtcgtcaat gtctccctta taaccgatta cgacagcggt ttggtcggca
     1561 acgtggaacc ggtttcccat tcggaagtgg taaaagtctt cggacaaaac atcagcaaac
     1621 tgcagaccct gctgaaaaac ttgatcggta ccatcccgga cggaagagcc aagtgcgact
     1681 gcgccaacac cctcaagctc gcccgcgtat gaggatctga gaaccgatgt ggcgcaactt
     1741 cactatcaat catgtagtag cgctcctggc tctggtactt tccattgcct catacctggt
     1801 attgcgccgt tatcacttcc ccgacaaaat ctgtgtcggg ggagcgatac tgtgcatact
     1861 cgtggtagca atattctggt cctgtgtgct cttaggcaca gaggaagatg acgaagagta
     1921 agcaaaaaac tccccacccc tgaggacagt aagtgttcaa acgcacatca aacctttgtt
     1981 tttccttgtg ttcttcttta gccttcctcg ctctggcagg cactgccggc gcccaggccc
     2041 aacccgcagc cgatgcaggt aaagcctcgc cgatcacaat taagaagccg ctcataccgg
     2101 taacaaaaga ctacaccctc aaaaacggtc tcaggctgat tgtctcggaa gatcactccg
     2161 ctcctgtagc ttctctagcc atcgtctacg acgtcggtgc ccgcgatgaa cagaagggcc
     2221 gctcaggctt cgcccacctc tttgaacaca tgatgttcca gggttcagaa aacgtaggca
     2281 aagtcgagca cttcaaatat atcgaaaacg tgggcggctc actaaatgcc tctacccatg
     2341 ccgacttcac caactatttc gagaaagtgc cctccaatca aattgagatg tgcctctggc
     2401 tggaatctga tcgtatgcgc tccctcaagg tcacccccga caatttcaag aatcagctcg
     2461 ataccgtcaa agaagaaaaa cgcatgcggg tggacaacca gccctatgca ccttcttccg
     2521 taagactgga agaactggcc ttcgacaact ggggcaacgg ccacccgacc attggttact
     2581 tcgaagacct ggaagcctct tccgtcaaag acgtcaaaca attcttcgat acctattacg
     2641 ctcccaataa cgccacc
//
LOCUS       DAOQNZ010000477         2655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21803_length_2655_cov_3.052308, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000477 DAOQNZ010000000
VERSION     DAOQNZ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21803_length_2655_cov_3.052308"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PKW73_09335"
     CDS             complement(<1..1370)
                     /locus_tag="PKW73_09335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA73530.1"
                     /translation="MPAEKEKKKFNAANTKPLTLDADTMKAADESPLALSAGSLLAQV
                     LHEEPLTRKPDADNQQKKQGKNGNSGKLAGKVKSLDNTDETRTKVLDDAFFTEDWQTQ
                     EGDLASSDGSAPATYAREAANKLIGTTVNDQYQIVGAIGYGGMSVVLKAKDLKLGKFV
                     ALKMLLPHLMLQSHSMARFQQEAHSASALNHPNVVSIHNYGETESGQPFLVMDLIEGS
                     SLASLIKREKHLSVDRALNIFIQLSSALCHAHQNGIVHRDLKPSNVLLCEQNGETDVC
                     KIVDFGIAKLLPHEGRDAVSLTQTGDVFGSPLYMSPEQCKGEKLDAKADIYSLGCLMY
                     ETLCGTPPHTGNNMLEVLYRHMNEIPKDFKSLKLDISVPQKLEALIFKTLAKEPSARP
                     ASMQAVHDELKQIFFYRKSSFLGRLVTSWELFVLKRRPRKKSEFIVLGALVVSLIALF
                     AAWAYL"
     gene            1667..>2655
                     /locus_tag="PKW73_09340"
     CDS             1667..>2655
                     /locus_tag="PKW73_09340"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNA73531.1"
                     /translation="MQTVEEQELRKGVRQESMPVSFFYRWMRSVLFEMDPEEAHALAI
                     QLAPVAAAMSSGPFVDPVLGTSLAGIKFANPVGLAAGFDKNGRLTNCLDRFGFGFAEI
                     GSITGQSTAGNPKPRLFRLIEDEAVINRLGLNGDGASVVAQRLSGAGFSLPVGVNIAK
                     TNLPSIKAEKGVADMLHSFECIKDLPLSFVTLNASCPNTHEGIMQETEELDQILSGVQ
                     RLNERKIPVLIKLSPDSTGELLETFVASARKYGINGYVLGNTTTTRDGLKVSAERLAS
                     IGNGGLSGKPLKEKALDLVRRVYKIKAKEQVIIGCGGISTGSDVFEFIVNGANA"
BASE COUNT          605 a          656 c          682 g          712 t
ORIGIN      
        1 atcaaatagg cccaggctgc aaacagagcg attagtgaaa caaccagtgc ccccagaacg
       61 atgaattcgc tcttcttgcg cggtctgcgc ttgagtacaa aaagctccca gctggtcacc
      121 agtcgaccga gaaaggaaga cttgcgatag aaaaatatct gtttcaactc atcatgcact
      181 gcttgcatgc tggcaggacg agctgaaggt tcttttgcca gggtcttgaa tatcagtgct
      241 tccaatttct gcggcacgct gatatccagc ttcaggctct taaagtcctt tggtatttca
      301 ttcatgtgcc tgtagagaac ttccagcata ttgttgccgg tgtgaggcgg tgtgccgcat
      361 agagtctcgt acatgagaca gcccagcgag taaatatcgg cttttgcatc aagcttttcg
      421 cctttgcact gttctggact catatagagt gggctaccga atacgtcgcc agtttgagtc
      481 agacttactg catcgcgccc ttcatgaggc agcagttttg cgattccgaa gtcgacgatc
      541 ttgcaaacat ctgtttcgcc gttctgctcg cacaaaagca cgttgctcgg tttcaggtcg
      601 cggtgaacaa taccattctg gtgggcgtgg cagagcgcag aagacaattg aatgaagata
      661 ttcaaggccc tgtccacgct cagatgtttc tctcttttga tgagagaggc cagcgaactt
      721 ccttcgatca ggtccatgac aagaaatggc tgtccgctct cggtctctcc ataattgtga
      781 atgcttacaa cattcggatg attgagagcg ctagcggaat gagcttcttg ctgaaaccta
      841 gccatactgt gcgactgcag catcaggtgg ggcagcagca tttttaatgc gacgaacttt
      901 ccgagcttca aatctttggc tttcagaacc acgctcatgc caccatagcc gatggcgcct
      961 acgatttgat actggtcatt aaccgttgtg ccaatcagtt tgttggcggc ttctcgtgca
     1021 taggttgccg gagcgcttcc gtcagaagat gccaggtcac cctcttgtgt ttgccagtct
     1081 tctgtgaaaa aggcgtcgtc gagaaccttg gttcgggttt cgtcggtgtt atccaggctt
     1141 tttactttgc ctgccaactt gcctgaattg ccgtttttgc cctgcttttt ctgctggttg
     1201 tcggcgtccg gcttccttgt cagcggttct tcatgaagga cctgagccag gagcgaaccg
     1261 gccgacagag ccagcggcga ctcgtctgct gctttcatcg tgtcagcgtc gagggtcagc
     1321 ggcttggtat tggccgcatt gaactttttc ttctctttct cggcaggcat tgctgccagc
     1381 cttgttccat cgtttggaca gatttttgtg tcagacgaaa agctctgccc gcattctttg
     1441 caggtgtttt caaccgtaat tctgcttgaa tcttctgcca aaatttcaat ccaaactgat
     1501 tgccagtctt ctagttttat ctgccgcaaa ggacacacgg cgcgatcaca tggatatctt
     1561 gcccgcaaaa ccttgctaat taatcattag ctcttactct tagatagtct aaagactatt
     1621 attttgtgtg attctttaag agagccaagc tgagatttca gagaagatgc aaacggttga
     1681 agaacaggag ctgcgaaagg gcgtgcgcca ggaatcaatg cctgtgtcat tcttctaccg
     1741 ctggatgcgt tcagtgctat tcgagatgga tcctgaggaa gcccatgcct tagcgattca
     1801 actggcgcca gtggcggcgg cgatgtcatc cggacccttt gtcgaccctg ttctcggcac
     1861 cagtcttgcg ggaattaaat ttgccaatcc ggtcggtttg gcagccggat tcgacaaaaa
     1921 tgggcggctg accaattgcc tggacagatt tggcttcggt tttgccgaaa tcggttcgat
     1981 taccgggcag tcgaccgccg gaaatcctaa accgcgcctt ttccggctta ttgaggatga
     2041 agctgtcatt aaccgtctgg gcctgaatgg cgatggtgct tctgtcgttg cgcaacgctt
     2101 gtctggcgcc ggtttttctc tgccggtcgg agtcaatata gccaagacaa atctaccgtc
     2161 aataaaagcc gaaaagggcg tagccgatat gcttcactca ttcgaatgca ttaaggacct
     2221 gccgctctcc tttgttactc tcaatgccag ttgtccaaac acccacgaag ggataatgca
     2281 ggagacggag gagctggatc aaattctgtc cggagtgcaa aggctgaatg agcgcaaaat
     2341 tccagtcctg ataaagctct cgcctgatag caccggcgaa cttttggaaa cttttgtggc
     2401 aagcgcccgc aagtatggaa tcaatggcta tgtgctcggt aataccacaa ccactcgcga
     2461 cggcttgaag gtatcggctg agcggcttgc ctccatcgga aacggtggat tgagcggtaa
     2521 accgctcaaa gagaaggctt tggacctggt aagacgggtc tataaaatca aggcaaaaga
     2581 gcaggtcata atcggctgcg gaggaatatc gaccggcagc gatgtttttg aatttattgt
     2641 caatggtgcc aatgc
//
LOCUS       DAOQNZ010000478         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21894_length_2648_cov_4.714231, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000478 DAOQNZ010000000
VERSION     DAOQNZ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21894_length_2648_cov_4.714231"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKW73_09345"
     CDS             complement(<1..547)
                     /locus_tag="PKW73_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73532.1"
                     /translation="MASFSTESSSKPTAKARVTKTKERPVSVAPELSKGVNSEVAQFQ
                     NILETAKSHSSKAQAKSQALTKETTLTFQAVNGTNAPISALIDSDGLVKRINFANGRA
                     IEMTAKMGKQAVYSDTGAPSVRLTNLAIDAVDGSIMYKRAMGKRTATMKETASGAKYA
                     TISMGREKREIQLLDDKRRVYK"
     gene            774..1514
                     /locus_tag="PKW73_09350"
     CDS             774..1514
                     /locus_tag="PKW73_09350"
                     /inference="COORDINATES: protein motif:HMM:NF027222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNA73533.1"
                     /translation="MKSVHEIAQEVFDTYGVRVITDQERTGLPIACRPADERTCLTYL
                     QQVLTRELSIYSPQVIRKSKLNEIILCQKLASEGQRRGGLTCAAFLGSLGLPFIRNKI
                     YLNIKVGGGWQYMRAAFHHELFHMIDYFDDINGYYDQRWKSLNRPGFVYHKDRIDEQR
                     AKPQRGFISEYAMDAVIEDKAEVYSYLLIDHRGTEKLVREDRILARKVVRMKELLHSF
                     ASEFNEDFWSKRDAASISYAKSIVKTRS"
     gene            1678..2295
                     /locus_tag="PKW73_09355"
     CDS             1678..2295
                     /locus_tag="PKW73_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA73534.1"
                     /translation="MRLLIVEDHEVTRDGICLAISREPELEIVGTADNSDAALALARE
                     LRPDVILLDLHLPGSLGPKSMVQAFCEIPDCRIVVFSNEHRQAFIDVVLKLGVAAYLL
                     KSEPSKVVIDTIRGVFNKGTNQVSPLAARPSTLKLTEAERQLLELFAHGLKYQDIADK
                     RFTTQSTVRKQCDLLLEKLGLENREALIAWAAKSGYGSLEEEEGK"
     gene            2300..>2648
                     /locus_tag="PKW73_09360"
     CDS             2300..>2648
                     /locus_tag="PKW73_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73535.1"
                     /translation="MNSLKQLTKAAESLARFLCDGGAGQSCGDDAALALSVVHQFFEE
                     LIQEFNSHPAVGDLQITITKPKSSLSPACESSANASKKVSKKASRTVFRFWIYSSAWA
                     LTVRTSRDYLEVFL"
BASE COUNT          612 a          625 c          707 g          704 t
ORIGIN      
        1 ctttatagac tcgtcttttg tcgtcgagca gttgaatttc tcttttctca cggcccatgc
       61 tgatagtggc gtatttggcg ccgctcgccg tttctttcat ggtggcggtg cgcttgccca
      121 tggctctttt gtacatgata gagccgtcga cggcgtctat agccagattg gtcaatctga
      181 cggaaggggc accggtatcg gaatagacgg cctgcttgcc catttttgca gtcatttcaa
      241 tggctctgcc gttggcgaag ttaatgcgtt tgaccagccc gtcgctgtcg atgagggcag
      301 aaatgggggc attggtgccg ttcacggctt gaaaggtgag ggtggtttct ttagtgagag
      361 cctgactttt agcctgggct ttagaactgt gactcttggc tgtttcaaga atgttctgga
      421 actgcgccac ttcagaattg acgcctttgg agagctcagg agccacgctc acagggcgct
      481 cttttgtctt cgttactcta gctttggctg tcggtttgga agatgattct gtagaaaaac
      541 tggccatttt atgtcacctg ctgaggagcc ggggggtgct cactttacat ctttgttata
      601 gcttgttaag aatgtaccta agaataacct tttggatatg ctcctgtcga ccctaaaatt
      661 tatatgccac tttcccaaaa gattaactcg tggcgttttt ccgagatggc aagcggaact
      721 aatattaaat ctgaggaatt acccccgact caggcttccg ggagaagcag cacgtgaaat
      781 cagtacatga gattgcccag gaggtatttg acacttacgg tgttcgagtt attaccgatc
      841 aagagcggac cggtttgccg attgcatgca gaccggctga tgagagaacc tgtcttactt
      901 atctgcagca agtgctgacc agggaactct ctatttattc tccacaagtt attcgcaaga
      961 gcaaactgaa cgagatcatt ctctgtcaga aactggcatc ggaaggccag cgtagaggcg
     1021 gtcttacttg tgcagccttc ctgggaagtc tgggtctacc cttcattcgc aataaaatct
     1081 acctgaatat caaagtgggc ggtggctggc aatatatgcg ggcggccttt caccatgagc
     1141 tgttccatat gattgactac ttcgatgaca tcaacggcta ctatgatcaa aggtggaagt
     1201 ctttgaaccg accaggcttt gtctaccata aagaccgcat tgacgagcaa agggcgaagc
     1261 ctcaacgggg ctttatctcg gaatacgcca tggatgccgt tatagaagat aaggccgagg
     1321 tctattctta tttgctgatc gatcacaggg gcactgagaa actggtccga gaagatagaa
     1381 ttcttgctcg gaaagtggtg cgcatgaaag aactcttgca ctccttcgcc agtgaattca
     1441 atgaagactt ctggagtaag agagacgcgg catccatatc ttatgccaaa agcattgtga
     1501 agacgaggag ctaggaacct cgccggaccg ggctggagcc ggttgccccc gcgcttggcc
     1561 cctcgttttg cccgagcatt ttgcctgccc tgcttcggta tactcatttt cgcccattta
     1621 cctggggctc tgccctgccc ctataatgta gtatccctgt ttggaatcga gcacagtatg
     1681 cgtttgctta tcgtagaaga ccatgaagtg accagggacg ggatttgcct ggcaatctca
     1741 agggaacctg aactggagat tgtgggtacg gccgacaata gtgatgcggc tcttgcactg
     1801 gcacgagaac tgcgtcctga tgtaatcttg ctcgacctgc atctgcccgg cagccttggt
     1861 cccaagtcaa tggttcaggc tttttgtgaa atccccgatt gccgcattgt ggttttttcc
     1921 aatgagcaca ggcaggcttt catcgacgtg gtgttgaaac tgggggtggc ggcctacctg
     1981 cttaagtcag agccctccaa ggtagtaatt gacacaatca gaggcgtctt taacaagggg
     2041 acgaatcagg tatctccgct tgccgccaga ccttccaccc tcaaactaac tgaggcggaa
     2101 aggcagcttc tggaactgtt tgcccatggc ttaaagtatc aagacattgc cgataagaga
     2161 tttactactc agtctacggt gcgcaaacaa tgcgacttgt tgcttgagaa gctagggctg
     2221 gaaaatcgag aagccttgat agcctgggct gccaaaagtg gttatggcag cctggaagag
     2281 gaagaaggga agtgaagccg tgaacagtct caaacaattg actaaggcgg ctgagagtct
     2341 ggcccgtttc ttgtgtgatg gcggtgccgg ccagtcatgc ggcgatgacg cggctctggc
     2401 actgtcggtg gtgcatcaat tttttgagga gcttattcag gaatttaatt cccatccggc
     2461 cgtaggtgat ctacaaataa ctattactaa gccaaagagt tcgctctcgc ctgcatgcga
     2521 atcttcggcc aatgcttcga aaaaggtttc gaaaaaggct tcgcggacgg ttttccgatt
     2581 ctggatatat tcgtcggcct gggctctcac cgtcagaact agccgcgact atctggaagt
     2641 ctttctgc
//
LOCUS       DAOQNZ010000479         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_21946_length_2645_cov_3.625869, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000479 DAOQNZ010000000
VERSION     DAOQNZ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21946_length_2645_cov_3.625869"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PKW73_09365"
     CDS             complement(<1..868)
                     /locus_tag="PKW73_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNA73536.1"
                     /translation="MPTSTKVQNYFGLTAQQAENDNARAVIIPAPYETLAKGSKDAAK
                     AILEASQELELFDDELWVEPYKIGIHSQSPLKIETVDNESENPFKEVTDAVKPVVEMD
                     RFPVVIGGERSISLGSVNACLDKYPNLSVLMLGSQADLRSEVDGNPYALGTTTYCLHT
                     SLKTPNIVQAGTRNISWEEVAWLEKEQPAINIFWARNQAKWDLNEIVSGLSENVLISI
                     DMSVLDPSIMPAVHHPEPGGWSWYPLLDLLKLVCVRKNVVAAEVTGLTPIKNLAAPNY
                     LAANLIYKLIGYR"
     gene            1211..>1598
                     /locus_tag="PKW73_09370"
     CDS             1211..>1598
                     /locus_tag="PKW73_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73537.1"
                     /translation="MAARRRKFASLFLTLWLGGFAASSPPAFALFYGFNQQAQSYQHE
                     GESLVQSGNYEQAVSAYTQAINLLPYDAVQGRADAYFGRAIANDVAGHFDQATQDYGW
                     SRDYYLKAAQYASYGGNSADSGLNGGY"
     assembly_gap    1599..1608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1609..2190
                     /locus_tag="PKW73_09375"
     CDS             <1609..2190
                     /locus_tag="PKW73_09375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNA73538.1"
                     /translation="QIGQELVNLVRWRATVNPRSSDYMDDAGPMKLWDLRKMPLKVFI
                     DDSQESGWSSDLRDLIWRAINCWTNVSGSPVRFMTWNSASGADYILTRPTAGGQIAVG
                     SGGYTSGVDEPSEPNMLKQSKSLLSCRGYDGNYYSQEDKNLLYNLALHECGHALGMGG
                     HSPSGQDIMYWKAPIFKLSDRDGNTIRRMYQGR"
     gene            2382..>2645
                     /locus_tag="PKW73_09380"
     CDS             2382..>2645
                     /locus_tag="PKW73_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73539.1"
                     /translation="MKLSKRQLDLQAGKLGLALVLAFTVGGASLAAPDMQGAIKKYNA
                     KDYQGALNDLKEFDKAKPNDGLCHYYMALCNQCLARVGEAKLHY"
BASE COUNT          578 a          650 c          763 g          644 t
ORIGIN      
        1 agcggtagcc gatcagcttg tagatcaggt tggcggcaag ataattgggc gccgccaggt
       61 ttttgatggg agtcaaaccg gtgacttcgg cggctaccac gtttttcctg acgcagacca
      121 gcttgagcag gtccaagagg ggataccagg accaaccgcc cggttccgga tggtgcacgg
      181 ccggcatgat ggagggatcg agcacgctca tgtcaatgga aataaggaca ttttcagaga
      241 gcccgctcac gatttcattg agatcccatt ttgcttggtt gcgagcccag aagatgttga
      301 tggccggttg ttctttttcc agccaggcta cttcttccca gctgatatta cgggtgccgg
      361 cttgcacaat gttgggagtt ttgaggctgg tatgcaggca ataggtagtg gtgcccaggg
      421 cataggggtt cccgtccact tccgagcgca ggtcggcctg gctgcccagc atcagcactg
      481 aaagattggg gtatttatcc aggcaggcat tgaccgagcc caggctgatg gaacgctcgc
      541 cgccgatgac aaccgggaag cgatccattt ccaccaccgg tttcacggca tcggtaactt
      601 ctttgaaggg attctcactc tcattgtcga cagtttcgat ttttagcggc gactgggaat
      661 gaatgccgat tttgtatggt tcaacccaga gttcgtcgtc gaaaagctcc agctcctggc
      721 tggcctctaa aatggctttg gcagcgtctt ttgagccttt ggccagggtt tcataagggg
      781 cggggattat taccgcccgt gcgttatcat tttcagcctg ctgcgcggtc agaccgaagt
      841 aattttgcac ttttgttgaa gtcggcatgt gctcccccag tagatttggc gttgaggctg
      901 tttgataggc tgcttggatc ttgtcaggct cttgaaaatg cctgcctgaa ccctttggta
      961 accaggcgtt tatagcatta tttgtcgtct cttggcatta gatagaggtc caatcacccc
     1021 ttctctggct ttgcccaggt ttgacccggc ggtatactat agctcgactt gggagcgcga
     1081 cctgggattt tgacttagta gcaccacctg gtgtcgtgac tgtaagctgg tttcccaagt
     1141 cgaacgggat gatggcgggt atttgatgtt ctcagcgggt aagatgctct caacgtgcaa
     1201 aaagtttggt ttggctgctc ggcggcgcaa gtttgcatct cttttcctca ccctttggct
     1261 gggcgggttt gcggcaagca gcccaccggc gtttgctctt ttctacggtt tcaaccagca
     1321 agctcagtct tatcagcatg agggcgagag cctggtccag agcggtaatt atgaacaggc
     1381 ggtctctgcc tacacccagg ctatcaatct cctgccctat gatgccgtgc aggggcgtgc
     1441 cgatgcatat tttggacggg ctatcgccaa cgacgtggcc ggtcattttg atcaggctac
     1501 tcaagactac ggctggtctc gcgattatta tctgaaagcg gctcagtatg cctcatacgg
     1561 aggcaatagt gccgacagcg gcctgaacgg cggttacgnn nnnnnnnnca gatcgggcag
     1621 gaactggtca acctggttcg ctggcgggct acggtcaatc ctcgcagttc cgactatatg
     1681 gacgatgccg gtcccatgaa gctctgggat ctgcgaaaaa tgcccttgaa ggtctttatc
     1741 gatgacagcc aggagtccgg ctggtcttcc gatttgcgcg atttgatctg gcgggccatc
     1801 aactgctgga ccaatgtctc gggctctccg gtgcgcttca tgacctggaa cagtgccagc
     1861 ggtgccgatt atattctgac cagacccact gccggcgggc agattgccgt tggttcgggt
     1921 ggctatacca gcggcgtgga tgagccttcc gagcccaata tgctcaagca atccaagagc
     1981 ctgctcagtt gtcgcggcta cgacggcaat tattacagtc aagaagacaa aaatcttctc
     2041 tataacctgg ctttgcatga gtgcgggcat gccctgggca tgggtgggca ctcgccttcc
     2101 gggcaggaca taatgtactg gaaggctccc atattcaaac tcagcgaccg ggacggcaac
     2161 acaatcaggc gcatgtacca ggggcgctga gtggtttggt taagcaggtg acgtgagcaa
     2221 caatagggcg gatgggaaca taatagtcga gaaaatcatc aagcagatgc aagtgataag
     2281 ggtccgaggc ataagtgaga gcatgagttt gattttgacg gtcactgacc gtcgctgaag
     2341 cgaaccgaat caagccgaat caagtaaggt acaggtagaa aatgaaattg agcaagcgcc
     2401 aactagattt gcaagcaggg aaactaggac tggcattggt tttggctttt acggtcggcg
     2461 gagcgtccct tgcggcaccg gatatgcagg gagcgataaa gaaatacaac gctaaagact
     2521 atcagggtgc cctcaacgac ttgaaagagt ttgacaaagc caagcccaat gatggtttgt
     2581 gccactatta catggccctg tgcaatcagt gtctggcccg ggtaggcgaa gccaagcttc
     2641 actat
//
LOCUS       DAOQNZ010000480         2641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22006_length_2641_cov_3.845708, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000480 DAOQNZ010000000
VERSION     DAOQNZ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22006_length_2641_cov_3.845708"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..769
                     /locus_tag="PKW73_09385"
     CDS             203..769
                     /locus_tag="PKW73_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA73540.1"
                     /translation="MVRNTYNDSALLLSLAACLLCAWPSVSAAETTEAKGKQLCHVKC
                     TDPHAACEESQKVIDTLNHLVKAMNEGDFSTVGEYMDENVTTFDEDSKKLLCGREAVL
                     AEMKKRYEKSVAQAAGGSVSYHIEQPYAQVSGDRATVTFLLRKTISGKTPVSLESHST
                     DVFVKHEGKWKKLHYRGSWKKVSPRLDS"
     gene            complement(747..1202)
                     /locus_tag="PKW73_09390"
     CDS             complement(747..1202)
                     /locus_tag="PKW73_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA73541.1"
                     /translation="MGIAACRVTVDVVALKAVPDDPALLLIERKHPPHQGCFALPGGF
                     LDDEDESLAQAAKRELLEETNVRVGLEQLSNFGTYGDKGRDPRGRTVTAAFLVELTES
                     QAEAMKAGDDAANAHFYALSALPELAFDHKQILADALKHRALLRSRDGD"
     gene            complement(1220..2065)
                     /locus_tag="PKW73_09395"
     CDS             complement(1220..2065)
                     /locus_tag="PKW73_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HNA73542.1"
                     /translation="MKGLKKQRDVLTSNIQQYRNTTAFGSDSDPNVPEKETFVSSFDG
                     QQGFYALSPPFMPSKAEDATLIVYFHGMGSTYMEPYLVPKDKSIAQVCRDQLRQGAFL
                     SVDYRGKASWLNQAALADVDQNVQAALLRFPARKIILVGTSMGGCSALSYSYLAAPDI
                     KEKIVGVLACEGAGDLVALYKETDSDLVRKGLIEAFGGLPETMGNEYAHHSILSNLAM
                     VNRKVRYVLLSASQDDVVPSALQKSMAEKLKGAVDSVELIEFPDRHGVPEAAVYEKGL
                     QALVR"
     gene            complement(2161..>2641)
                     /locus_tag="PKW73_09400"
     CDS             complement(2161..>2641)
                     /locus_tag="PKW73_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73543.1"
                     /translation="TDADPLNGCMYILPAHQDINYPNNLTTMGVNNLQDVRAIPCKAG
                     SVVMWNEAVYHWGGRSSKYAGHTRLAMALAFQRSDQEPFETPLLKALSLPGFEARLSL
                     IAYQMLRYQAQGALSPVLRELCVQLAALGEPLVVHDQGGPYFYDGMGEYTGSRRTAP"
BASE COUNT          612 a          729 c          712 g          588 t
ORIGIN      
        1 agccgagatg cgttgcttga tcaacgaaaa tatctgcaaa gtgaagctga caggctcagc
       61 caacaggtgc agcttttgca acagaagcta gatcgcgtca acgcctatat gcgcgacaac
      121 gaccgagctt tacgcgatgt ggaaaccgcc atgagacagg cacagtaagt attcacggaa
      181 ctggcattgc cggaggtaga gaatggtaag aaacacatac aacgattcag cacttctgct
      241 cagtctggcg gcctgcctcc tctgtgcctg gccatcggtg tcggcggcgg agaccactga
      301 ggccaagggc aagcaactct gccatgtgaa gtgcacagac ccccatgcgg cctgtgagga
      361 atcccagaag gtaatcgaca ccctcaatca tctggtgaaa gccatgaacg aaggtgattt
      421 ctccaccgtc ggtgaataca tggatgaaaa tgtcaccacc ttcgatgaag atagcaagaa
      481 gcttctatgc ggtagagaag ctgttctggc tgaaatgaag aagcgctatg aaaaatccgt
      541 ggctcaggcc gcgggcggct ccgtttctta tcatatcgaa caaccttatg cccaggtgag
      601 cggcgatagg gccacagtca ccttcctgct gcgtaagacc ataagcggca agacacctgt
      661 ctcacttgaa tcccatagca ccgatgtctt tgtaaaacac gagggtaagt ggaagaaact
      721 ccattaccgg ggcagttgga aaaaggtcag tccccgtctc gactcctgag cagggctctg
      781 tgcttgagcg catctgccaa tatctgtttg tgatcaaagg ctagttccgg tagcgccgac
      841 agggcgtaga agtgcgcatt ggccgcatcg tcaccggctt tcatggcttc cgcctggctt
      901 tcagtcagct ccaccagaaa ggcggcagtc acagtgcgtc cccgcggatc gcgtccttta
      961 tcaccgtaag tgccgaaatt gctcaattgt tcgagcccaa cccgcacatt ggtttcttcc
     1021 agcaattctc ttttggccgc ctgcgccagt gattcgtctt cgtcatcgag gaagccgcct
     1081 ggcagggcga agcagccttg atggggcgga tgtttccttt ctatgagcag tagggccgga
     1141 tcatccggca ctgctttgag agccaccaca tccaccgtta cccggcaagc cgctatgccc
     1201 attcttatct cctctgtact tatctgacca gggcctgcag acccttttcg tagacggccg
     1261 cttccggcac cccatgccgg tccggaaact caattagttc cacggagtca acggctccct
     1321 tcagtttttc cgccatggat ttttgcaggg cgctgggcac cacgtcgtcc tggctggcgg
     1381 aaagcaagac ataacgaact ttgcggttga ccatggccag attgctcaag atagagtggt
     1441 gggcatattc attgcccatg gtctcgggaa gaccgccgaa ggcttcgatc aagccttttc
     1501 tgaccaggtc cgaatcggtc tctttgtaga gagccacaag gtcgcccgct ccctcgcaag
     1561 caagcacgcc cacaatcttt tccttgatgt ccggtgccgc cagataagag taagaaaggg
     1621 ccgagcagcc acccatagag gtgccgacta agatgatttt gcgtgccgga aatcgcagca
     1681 atgccgcctg cacattctgg tccacatcgg caagggcggc ctggttcagc caggaggctt
     1741 tgcctcggta atctactgaa agaaaggctc cctgtctcaa ttgatcccgg caaacctgag
     1801 cgatactctt atcctttggt accagatatg gttccatata tgtggagccc atgccgtgga
     1861 agtaaactat cagggtggca tcttcagcct tactgggcat aaatggcggc gagagcgcat
     1921 aaaagccctg ctgcccatca aagcttgaca caaaggtctc tttctcaggc acattaggat
     1981 cactgtctga gccgaaggca gtggtgttgc gatattgctg gatgttactg gtcagtacgt
     2041 cacgctgttt cttcagaccc ttcatcttcc cggcgctatt caggtagagt aagaagaaaa
     2101 ccagaccggc gacatcgatt aagacagcta caaccagggc aactatgagt attttcttgc
     2161 ctatggtgcc gtcctccggc taccggtgta ttcacccatg ccatcataga agtagggacc
     2221 accttgatcg tggacgacca ggggctcgcc cagggccgcc agttgcacgc ataattctct
     2281 cagcaccggt gaaagcgcac cctgagcctg gtagcgcaac atctggtagg caatcaagga
     2341 aagacgcgct tcgaagccgg gcaggctcag tgcttttagc aagggtgtct caaatggctc
     2401 ctggtcggag cgttgaaagg ccagggccat agccagcctg gtgtggccgg catatttgct
     2461 gctgcggccg ccccagtgat aaacggcctc attccacatc accaccgagc cggccttgca
     2521 ggggatggct cgcacgtctt gcaggttgtt aacccccatg gtagtcagat tgttgggata
     2581 gtttatgtct tgatgggcgg gcaaaatgta catacagccg ttcaggggat cggcatccgt
     2641 g
//
LOCUS       DAOQNZ010000481         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22112_length_2635_cov_2.990698, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000481 DAOQNZ010000000
VERSION     DAOQNZ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22112_length_2635_cov_2.990698"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1325
                     /locus_tag="PKW73_09405"
     CDS             177..1325
                     /locus_tag="PKW73_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNA73544.1"
                     /translation="MTDSLSAEMSSNHCLLTIGELVVDWIATSKGASWSKPDTFLRSP
                     GGNAANVATAYARLGGQSRLVAKVGSDIHGRYLLETLGDSGIDTRFIFETSRYPTAQC
                     YVLTGEDEENIFFNWPKPNACHQLTVKELSPDMFEGAFALHATGISLTVEPRSHFVIA
                     AMRRARELGLLISFDAGFPYGEGELARKLTREAMSLSHMIKVNLPELIYWLGRSKHEV
                     PPADLAKSPTKPGHLDILLSYSRDLMNQTGAHVVLCTLGAHGSIVVTDQKEKYYEPLR
                     VKSLAGVGAGDAYVAAVLYQLSDHYQKLLALPDGPRRHSVLLPAKMQAQAQKAPADTT
                     VRPADILELDWDQVAVFANATGALATRTVSASAGLPNCAEVAELLNKR"
     gene            1470..1610
                     /locus_tag="PKW73_09410"
     CDS             1470..1610
                     /locus_tag="PKW73_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73545.1"
                     /translation="MEAIQNIFGLFHEMALFAVISLVVTGITVTAVLFKVFKSDFAQF
                     DP"
     gene            complement(1622..2251)
                     /gene="yedF"
                     /locus_tag="PKW73_09415"
     CDS             complement(1622..2251)
                     /gene="yedF"
                     /locus_tag="PKW73_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HNA73546.1"
                     /translation="MKKQLDLRGLTCPEPVLKTKKVVDQADVKEVEALVDSEVSVQNL
                     ARLARSQNLHFQSSRENGHHKVLISKDQTGTVQTQSETAAAATRDGAGGTIIFISKDS
                     FGQGNSASPEKDHDFSLNLLNVFLQTVHQSGHEVRAVLLANSGVKLMDPQSPHQQVLS
                     DLARGGVEVLACGLCLDYYGLKEKVKTEQITNMFAICEYLFAADKVLSP"
     gene            complement(2286..>2635)
                     /locus_tag="PKW73_09420"
     CDS             complement(2286..>2635)
                     /locus_tag="PKW73_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNA73547.1"
                     /translation="HIQEMSKASKVSATINIKHLRFLPGVLEFARQGLVPAAAYGNRK
                     SFETNVSYIKDFPLEFTDLLYDPQTAGGLIFAVANEHAEACLKALNGSGVAATIIGQF
                     LEGIPGHIDITMN"
BASE COUNT          574 a          703 c          681 g          677 t
ORIGIN      
        1 gcccttttgc aaggcaagat tgtatctaag ggaccggtca gcaaagccct ggcacttcta
       61 ccggccctta agccggccta tcccatgtat cccaccgtca tcaagaataa cggcttgaaa
      121 gtctctgtct gagctcgggc tctcagattt agtacccctt ttggaaacac tgaacgttga
      181 cagactcttt atccgctgag atgagctcca atcattgctt gctgaccatt ggggaattgg
      241 tggtcgattg gattgccact agtaaaggtg cctcctggtc caagcctgat acctttttga
      301 gatcccccgg cggtaatgcc gccaatgtgg ctacggccta tgccaggttg ggcggacaat
      361 ccaggctggt agccaaagtc ggcagcgata tccatggtcg ctacttgctt gagactcttg
      421 gtgattcggg cattgatacg cgctttattt tcgagaccag tcgctatcct acggctcagt
      481 gctatgtcct gaccggggaa gacgaagaaa acatcttctt caactggccc aagcccaatg
      541 cctgccatca acttaccgtg aaggaacttt caccggatat gtttgaaggg gcttttgcct
      601 tgcacgccac aggcatttca ctgaccgtgg aaccgcgcag ccactttgtc attgcggcta
      661 tgcgccgggc cagagagctg ggtctcctga tttccttcga tgccggcttt ccatacggtg
      721 agggcgaact ggccagaaag ctgaccagag aagccatgtc tctctctcat atgatcaagg
      781 tcaatctacc cgaacttatt tattggctcg gcagaagcaa acatgaagtg ccgccggcag
      841 acctggccaa gtctcccacc aagcccggcc atctggatat tctcctctct tatagtcgcg
      901 acctgatgaa ccagaccggg gctcatgtgg ttttgtgcac ccttggcgcc catggttcca
      961 ttgttgtcac cgaccagaaa gagaagtatt acgaaccgct cagggtgaag tcgctggccg
     1021 gtgttggtgc cggcgatgct tacgtggccg ccgtacttta tcaattgtcc gaccattatc
     1081 aaaagctcct ggctctgcct gacggtccca ggcggcactc ggtgctttta ccggccaaga
     1141 tgcaggccca ggcgcagaag gcaccagccg ataccaccgt gcgtccggcc gatatactgg
     1201 agttggactg ggatcaggtg gctgtctttg ccaatgccac cggagctctg gctactcgca
     1261 cggtcagtgc ttcggccggt ctgccgaact gtgccgaagt ggctgaactc ttgaacaaaa
     1321 ggtagtctgt aatacaaaaa tgcctgattt tcccccgggg agcttcaaat acgtatatac
     1381 cggccttgaa aatgtgccgg aaggtgctaa cctgcaactc tcccctaaac tcatttgagt
     1441 taaatttcgc gaaaattcag gtatctgcca tggaagccat acaaaatata ttcggtcttt
     1501 tccacgagat ggcactgttt gccgttattt ccctggtagt gaccgggatt acggtgacgg
     1561 ctgttttatt taaggtcttc aaaagcgact ttgcccaatt cgacccttaa gttcatgaaa
     1621 ctcaggggct gagaactttg tcggcggcaa agagatattc gcaaatggcg aacatattag
     1681 tgatctgttc ggttttcact ttctctttca gtccgtaata atccaggcac aagccacagg
     1741 ccagaacctc cacccctccc cgggccaggt cgctgagaac ctgttggtgc gggctctggg
     1801 ggtccatgag cttgacgccg ctattggcaa gaagcacggc tcttacttca tgaccgctct
     1861 ggtgcacggt ttgaagaaag acgttgagta gattcaagct gaagtcatga tccttttcgg
     1921 ggctggcgct atttccctga ccgaaactat ctttgctgat gaaaataatg gtgccgcctg
     1981 caccatccct ggtagcagct gcagctgtct cactttgggt ttggacggtt ccggtctgat
     2041 ctttggaaat gagcaccttg tgatgaccgt tctctctgct gctctggaag tgcaggttct
     2101 gggagcgagc cagcctggct aaattctgca cgctcacttc cgagtccacc agcgcttcca
     2161 cttccttgac gtcggcttga tccacaactt tcttggtctt caagaccggc tccgggcagg
     2221 tcaatcctct taagtccagt tgctttttca tttcattctt cctgattgga accgagcgac
     2281 ttgcatcagt tcatggtgat atcgatgtga ccggggatgc cttcaagaaa ttggccgatg
     2341 atggtggcgg ccacgccgct gccgttaagg gccttcaagc aagcctcggc atgttcattg
     2401 gctacagcaa agatgagccc gccggctgtc tgcggatcgt aaaggagatc ggtaaactcg
     2461 agagggaaat cctttatata ggatacgttt gtttcgaagg acttgcgatt accgtaggct
     2521 gccgccggca ccagaccttg cctggcaaat tccagaacgc ccggcaagaa acgcaagtgt
     2581 ttaatattga tggtagcgga tactttgctg gctttggaca tttcttgtat atgtc
//
LOCUS       DAOQNZ010000482         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22122_length_2634_cov_3.281504, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000482 DAOQNZ010000000
VERSION     DAOQNZ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22122_length_2634_cov_3.281504"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1330)
                     /locus_tag="PKW73_09425"
     CDS             complement(125..1330)
                     /locus_tag="PKW73_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA73548.1"
                     /translation="MAAPARPGQRLGEKRARLAPLPLLPLLLQSLMQLCPGTEQTVLA
                     EPVRTSRAPAEKKDASAVKSLKLYGRVDELSSTMHGAGLKIESLTLPSRVVKVRMGSP
                     AYYAGMAEEDLIEKMTLNAGTLQVVFRRNGKIYGANIATAPPAQTNNTNTNTPLRPPA
                     ARSTRTLKTQIADWSYLKDYDICVLIDRSGSMEDEIEGGRQTKWQWMQTIVRDFAFEA
                     KQFAGKSLTVLTFNRQFKVTENVTPASLYQVFNGLTPEGGTDLYSPLAYCLERQAGSN
                     PPKPLLVAVITDGEPDAYEQVAALIRAATARQKRQTDLQITFLGIGENTIGEGVLNYF
                     DDALVSQGARFDVVDSISFEDLKQLGIAAALMEAFRRPRANQARYSDNELARLLKKLQ
                     NQAAREGGH"
     gene            complement(1330..2331)
                     /locus_tag="PKW73_09430"
     CDS             complement(1330..2331)
                     /locus_tag="PKW73_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73549.1"
                     /translation="MPEVDADRIMPTKSTSLSILKPALSCLVCLACALWEPAAGQEAT
                     MQTLEKAYQDGNLKAAAGMAKELIGKNRNNLTARYYLGNIYTKLGLAHEAMSEYAYCV
                     NFGKDSQVAGYAREAMKSIDQVLSDRPPTAVGKQTAGGAGAGNTSKTALQLKTELLTR
                     GSQEVEDLKEKHRQAVAALKKETDAKAAPYLPPSLRNWDKGISPREQLAITDDPNYRH
                     FLELQIELSEKIYKLNQKLNDDIERVNESYKRRVEAIDQYGGTAAAAQAQILPEQGGT
                     LRNYVNYGKDEEIDTIPVTGALTATPASLEQGQKQKPKTPTRASKKQNPTTSRKKAP"
BASE COUNT          436 a          698 c          789 g          711 t
ORIGIN      
        1 cccgctgatc ttttactcgc tcactgaggg tggtgcgctt tgctcagaca agattccagc
       61 ctcagccctt acctctttcc ggtagggatt aagcgtccca catttccgct tgcgccccgg
      121 gcgatcagtg accgccttct ctcgctgcct gattttgtaa cttcttgagc aggcgtgcca
      181 gttcgttatc gctgtagcgg gcttgattgg cacgggggcg ccggaaggct tccatgagag
      241 cggcggcaat gcccagttgt ttgaggtctt cgaagctaat ggagtcgacc acgtcgaacc
      301 tggctccctg actgaccagg gcatcgtcga aatagtttag aacgccttcc ccgattgtgt
      361 tttctcctat gcccaggaag gtaatctgca ggtcggtctg acgtttctgt ctggctgtgg
      421 cggcccttat cagggctgcc acctgctcat aggcgtccgg ttcgccgtcg gtgatgacag
      481 ccaccaacag cggcttgggt ggattactgc cggcctggcg ttccaggcag tatgccaggg
      541 gcgaatagag atcggtgccg ccttccgggg tcagaccgtt gaagacctga taaagagaag
      601 ccggcgtcac attctcggtc actttgaact ggcggttgaa ggtcagaacc gtgaggctct
      661 tgcccgcgaa ctgtttggct tcgaaagcaa aatctcgcac aatggtttgc atccattgcc
      721 atttggtctg gcggccgcct tcaatctcgt cttccatgga gccggagcgg tctatcagta
      781 cgcagatatc gtagtctttc aaataagacc agtcggcaat ctgggtcttg agtgtgcgag
      841 tgcttctagc ggcaggaggg cggagcggcg tgttggtatt ggtattgttg gtctgggcag
      901 gcggggcggt ggcgatattg gccccgtaaa ttttgccgtt gcgcctgaac acaacttgca
      961 gagtgcctgc attgagggtc attttttcga tcaagtcttc ctcggccata ccggcataat
     1021 atgccggaga tcccattctt acttttacta cacggctggg caaggtgagg ctttcgattt
     1081 ttagtcccgc accgtgcatg gtgctactca attcgtccac ccgaccgtag agcttaagcg
     1141 atttgacggc cgaagcgtct ttcttttcgg ctggggcgcg gctcgtcctg accggctcag
     1201 ccaggacggt ctgctcagtg cctggacata gttgcatgag agattgcagc aggagcggta
     1261 gtagtggcag aggcgccagg cgcgcccgtt tctcgccaag cctctggccg ggtcgggcgg
     1321 gggcagccat cagggcgcct tcttccggct tgtggtgggg ttctgtttct tgctggccct
     1381 ggtgggggtt ttcggctttt gcttttgccc ttgttcgagg ctggccggtg tagcagtcag
     1441 tgctcccgtc accggtatgg tgtctatttc ctcgtctttg ccgtagttga cgtagtttct
     1501 gagggtgcct ccctgctccg gcagtatttg agcctgggcg gcagcggcgg tgccgccgta
     1561 ctgatcgata gcctctaccc ggcgcttgta ggattcgttt acccgctcta tgtcatcgtt
     1621 tagcttttga ttgagtttgt agatcttttc cgacagttcg atttgcaact ccaagaagtg
     1681 tctgtagttt gggtcgtccg tgatggccaa ctgttccctg gggctgatgc ccttatccca
     1741 attgcgcagg gagggcggca ggtagggagc ggctttggca tcggtttcct ttttcaaagc
     1801 ggccaccgcc tgccggtgtt tttccttcaa atcttctact tcctgggagc cccgggtcag
     1861 tagttccgtc ttcaattgca aggccgtttt gctggtgttg ccggcgcccg caccgccggc
     1921 tgtctgtttg ccgacggcag tgggcgggcg gtccgacaag acctgatcga tgctcttcat
     1981 ggcctctctg gcatagcctg ctacctggct gtctttgccg aagttgacgc agtaggcgta
     2041 ttcggacatg gcttcatggg cgagacccag ttttgtgtaa atattgccca gatagtacct
     2101 ggccgtcaaa ttgttgcggt tcttgcctat tagttcttta gccatgcccg ctgctgcttt
     2161 cagattgccg tcctggtagg ccttctcgag ggtttgcatg gttgcttctt gcccggcggc
     2221 cggttcccac aaggcacaag caaggcagac cagacaactg agggccggct tgaggatgga
     2281 aaggctggtc gactttgtcg gcattattct gtcggcatct acttcaggca aaaactgggg
     2341 gtctcacggg ttatgcgtca cttaaaacga ctagaagcat tataaacttt ggtgccggtc
     2401 aattgtcggt agttcggggc gaaccgccgc gctggtgtta ccatagaagt ttagatcgcc
     2461 gttagtatca ggattcccgt gtcagtcacg tttgccgtag attgtggtag tttgccgccg
     2521 cccgagcagt ttcttctgga cctggcggag cgtttgcaat ggtcgcctat ttgctgctcc
     2581 ggcttctttg atgaaggtca gatcatgagc aaccgggctc gcagctggca gcag
//
LOCUS       DAOQNZ010000483         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22201_length_2628_cov_4.096386, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000483 DAOQNZ010000000
VERSION     DAOQNZ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22201_length_2628_cov_4.096386"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKW73_09435"
     CDS             complement(<1..285)
                     /locus_tag="PKW73_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73550.1"
                     /translation="MSDRVERAVEAVNSGQNLTRVVNNEMTREEQLQFARQLWENGTN
                     RTPQAPGVWTGTVGGAEVTFRTVNGERQVSDIVLNGRDIYDTPEDVARRQG"
     gene            complement(379..1089)
                     /locus_tag="PKW73_09440"
     CDS             complement(379..1089)
                     /locus_tag="PKW73_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73551.1"
                     /translation="MQEVNRTQEASICDTGAERSLSSKIYDECGSGFLPEANKRVNDN
                     GLGQLGFPSVEFSGLDKGSGGKKDGLKELAERSDRPVDQFSTGEGAGSSAAEQLRRVV
                     EGQMSPQERRQYEKENEAMAKYKQELSKWGLLATIEPSPMPKAPDCPMHETVNKRMRD
                     TERVIEKQVRDGMSPEEQRQLDKEYREYKEALKKDREPGDPLGTGGLTRPGARPGSAV
                     NAYYRRVNEAVDAYARRH"
     gene            complement(1245..1682)
                     /locus_tag="PKW73_09445"
     CDS             complement(1245..1682)
                     /locus_tag="PKW73_09445"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HNA73552.1"
                     /translation="MFVYRICCAPHSALDGEGARLFGGRWNSPGLPAIYTSSHLSLAA
                     LELLVHLNFDNLPADLVWLKIEVPETGSMETFPASSAPSEKESFRFGDAWLSSGRSLA
                     LVVPSAVISVERNLILNPRHPQMSLVKTVELAPFQFDKRLFGS"
     gene            complement(1682..2110)
                     /locus_tag="PKW73_09450"
     CDS             complement(1682..2110)
                     /locus_tag="PKW73_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNA73553.1"
                     /translation="MDRDRVVKVILGCSELPPDVSSDMEMLKLLNEGLPADTLSTIEK
                     LGIIDGAELKSFIPARTLSRRRGAKQRLSIEESDLIARLARTHEFAVEVFGDKEKAKR
                     WLRTANRAIEGRLPLDLLRTDYGARIVDSLLGRIAHGIYS"
     gene            complement(2247..>2628)
                     /locus_tag="PKW73_09455"
     CDS             complement(2247..>2628)
                     /locus_tag="PKW73_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA73554.1"
                     /translation="ESLGGGVAAHVASKRQVGAVILQSTFASLPHVARQKMLLMRLYP
                     DFLFPKNKLDTAGIMAEPHAPLLIVHGKADSIIPFTESELLYKKAVEPKQLVAIESAN
                     HNDMYDTYVGSLEQAVAAFLDRLF"
BASE COUNT          595 a          748 c          663 g          622 t
ORIGIN      
        1 gccctggcgt ctggccacat cttccggcgt atcgtaaatg tctctgccgt ttagaacaat
       61 gtcgctgacc tgtctttcgc cattgactgt gcgaaaggtc acttcggcgc cgcctaccgt
      121 gccggtccat acgccgggag cctggggagt gcgattggtg ccgttttccc agagctggcg
      181 agcgaattgc aattgctcct ctctggtcat ctcattgtta accacgcggg taagattttg
      241 tccgctgttt acagcctcta cggctctttc aacacgatcg ctcatggtaa ttgctccatt
      301 tataaaagaa aatcggggtc ggttctgaat agactttatg cgacccaact tggcagacca
      361 gggtcacggt aattaaagtt agtggcggcg tgcataggca tccacagcct cattcacccg
      421 ccggtaataa gcattgacgg cgctgcccgg tctcgccccc ggtcgagtca agccgccggt
      481 gccgagcggg tcacctggtt cgcgatcttt cttgagagcc tctttgtatt cccgatattc
      541 tttgtcgagc tgccgctgct cttcggggct catgccgtcc cttacctgct tctcgataac
      601 acgctctgtg tcgcgcatgc gcttattcac ggtttcatgc atgggacaat caggggcttt
      661 cggcatgggc gaaggctcta tggtggcaag tagcccccac ttagaaagct cttgcttgta
      721 tttggccata gcctcatttt ctttttcata ctgacggcgc tcctgggggc tcatttgccc
      781 ttccaccacg cgccgcagtt gctctgcggc agagcttccc gccccttcac cggttgagaa
      841 ttgatcgaca gggcggtcgc tgcgctcagc cagttccttc agcccgtctt tcttgccgcc
      901 gctcccttta tctaagccgc taaactcgac actggggaaa cccaattgcc ccagaccgtt
      961 atcattgacg cgcttattgg cctctggcag aaagcccgag ccgcactcat cataaatctt
     1021 gctgctaagc gacctctccg caccggtatc gcagatagaa gcttcttgag tacgattaac
     1081 ttcttgcatg tctggcactc ctggtaagaa aattgccttt cgcgccgacc ggaaacggtc
     1141 ttgctcggca gattcacttt aggaggtgaa gccgtcaaac cagggtcaga aagattaaaa
     1201 cagacacggc ggatgtagcg tggcgctgaa gcgactgctt aggctcaaga accgaagagt
     1261 cgcttatcaa attggaaggg cgcaagctct acagtcttga ccaaagacat ttgaggatgc
     1321 ctgggattga gaatcaggtt cctctccacc gatatcacag ccgaaggcac aaccaatgcc
     1381 aggctgcgcc cgctactcag ccaggcatca ccaaaacgga aagactcttt ctcagatggt
     1441 gcagaggaag cagggaaagt ttccatgctg ccggtttcgg gcacttctat cttcaaccaa
     1501 acaagatctg caggcagatt gtcaaagttc aaatgcacaa gcaattctag agcagcaagg
     1561 gaaagatgcg atgaagtgta gatcgcaggc agaccgggag agttccacct gccgccaaat
     1621 aaccgcgccc cctcaccgtc caatgcgctg tgaggcgcac agcaaattcg atagacaaac
     1681 atcaggaata aatgccatgg gcaattctgc caagcaaaga atccacaatc ctggcaccat
     1741 aatcagtgcg caacaaatcc agcggcaatc tgccttctat agctcggtta gccgtgcgca
     1801 gccacctttt cgctttctct ttatctccga acacctcgac ggcgaattca tgagttctag
     1861 ccagtctggc aatcagatcg gactcttcta tgctcagccg ctgcttggca ccacggcgcc
     1921 gggatagggt tctggcggga atgaaagact tgagttcggc gccgtcaatg attcccaact
     1981 tttcaatagt agagagagta tctgcaggca gcccctcatt caatagtttg agcatctcca
     2041 tatcggagct tacgtccggc ggaagttccg aacatcccaa tataactttg acgactctgt
     2101 ctctatccat gaacttcaca actcttgccc gcagctctgg cgattcgcct tttgccttca
     2161 atcataacgc cacttggcgc gggcgacaag tggtgggctg aaaacaaagc tccagcgggc
     2221 aaagttgtgc cacccggcag cgccgttcag aacagccggt ctaagaaagc cgccaccgct
     2281 tgctcaagag agcccacgta ggtgtcgtac atatcgttgt gattggcaga ctctatggct
     2341 actagctgct ttggctcaac cgcctttttg tatagcaact ccgattcggt gaagggaatg
     2401 atgctgtcgg ctttcccgtg cacaatcagc agtggcgcgt gaggttcggc cattatgccg
     2461 gctgtatcaa gcttgttttt tgggaagaga aaatcagggt aaagccgcat taacagcatc
     2521 ttttggcgag ctacatgggg caaagaggca aaggtcgact gcaagatcac agcccccacc
     2581 tgccgcttcg atgccacatg agcagccacg ccgccaccca gagattcg
//
LOCUS       DAOQNZ010000484         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22217_length_2627_cov_2.931960, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000484 DAOQNZ010000000
VERSION     DAOQNZ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22217_length_2627_cov_2.931960"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PKW73_09460"
     CDS             complement(<1..1146)
                     /locus_tag="PKW73_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73555.1"
                     /translation="MTSIGKWWATMTEPGGSQLQMMLVQQAGPGALPLSILSPAEFLP
                     FIRDVEQLAVAEKLAPVLVYWSRRSAAWLLPTGPRFKELMRDAFCVSIFSEDRQDTPD
                     EWCFLIESRGLCLILYAQQALESPQGEKYQCSGSMNAQIVRQAFNRLLPAWQSIELGE
                     SNRLEDARINLGPCGSANHFVDRIRNLWPVVKSPIQQNLILKPQEVPQQPPIIVDHGK
                     VSPIALESGGFPGSAVVAPPRPLPAQQPGASALVPQAPMMVPGTGSGGDGGNDQSKAQ
                     QVRTQPLPKPSAFELPKPKQLKDQEYLNTAESEFETTGDLTEKLGAVIGPDGQPLPPR
                     PSALRGLRQTLADENQAAQQDDVIIPVPAQAIISDIIGQLRHSSDLTS"
     gene            1382..2074
                     /locus_tag="PKW73_09465"
     CDS             1382..2074
                     /locus_tag="PKW73_09465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA73556.1"
                     /translation="MNVDKAIIEEAPKKASQLRSSQVYKDNLEFWHQAWNMCTKPYTK
                     MPELEYLPWIPERLHAASAKSVLDLGCGSGWLAVYLSREGFRVTGIDVADHALELGKQ
                     WAQMENLEIRFDVGDLGDLPYSDNSFDAVVANSIIEHLPFSLALVAMTKLKSVMRSGG
                     TFIGCFDKVGTGPGEYFKLEDGTQVYTDKSRKGMMLRYFPDEELRELLTGWKIDEFKT
                     LDSGTRFVVAHT"
     gene            2117..>2627
                     /locus_tag="PKW73_09470"
     CDS             2117..>2627
                     /locus_tag="PKW73_09470"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73557.1"
                     /translation="MQGINKSPNEESCREALQDAEARLGGDDAQLVPFLDQLAYAYHS
                     TGKYAEAEECYHRAIKITEKKEGAKSPLLIPRLHNLAVLYRIQEKFPEAEQIYIRTIL
                     LTEKLPPEKILDLATQKNFLAGLYFAWGRLPDAENLVNDSLEIYKKTLGAEHEYASFC
                     LMGFCLIYKQ"
BASE COUNT          652 a          696 c          707 g          572 t
ORIGIN      
        1 ggaagtcaga tcgctcgaat ggcgcaattg accgatgatg tcgctgataa tcgcctgagc
       61 cggcacagga atgatgacat catcctgctg ggcggcttga ttctcgtctg ccagggtctg
      121 tctcaatccg cgcagagccg aaggacgagg aggcagaggc tgaccgtccg gtccgatgac
      181 ggcaccgagc ttttcggtca aatctcctgt agtttcaaat tcagactcag ctgtattgag
      241 gtattcctgg tccttcaatt gcttgggctt gggcaattcg aaagcggaag gcttgggcag
      301 aggctgcgtt cgtacctgct gcgccttgct ctgatcattg ccgccgtctc ctccagagcc
      361 cgtacccggc accatcattg gtgcctgagg gacgagtgcc gaggcaccag gctgttgagc
      421 cggtagaggg cgcggcggcg ccacgacggc tgagccggga aatcctcccg actccagtgc
      481 aatcgggctg acctttccgt gatcgacgat gatcggaggc tgctgcggca cttcttgagg
      541 tttcaaaatc agattttgct ggataggcga tttcaccacc ggccagagat tgcggattct
      601 gtctacaaaa tgattggcac tgccgcaagg gcccaggttg atgcgggcat cctccagtcg
      661 gttactctcg cccagctcga tagactgcca ggcgggcagc agacggttga aagcctggcg
      721 tacgatctgg gcgttcatgg acccagaaca ctgatatttc tcaccctgtg gagactccag
      781 agcttgctgg gcgtaaagaa tcaggcaaag accgcgactt tcaatgagaa aacaccactc
      841 gtcgggagtg tcctgccggt cttccgagaa gatcgaaacg caaaaagcgt cgcgcatgag
      901 ctctttgaac cttggtccag taggcagcaa ccaggcagcc gaacggcgcg accaataaac
      961 caggacgggg gcgagctttt cggctacagc caactgctcg acatcgcgga tgaaaggcaa
     1021 aaattcggct ggcgaaagga tgctaagcgg caaagcgccc ggcccagctt gctgaaccag
     1081 catcatctgc aattgtgaac cgccaggctc cgtcatcgtc gcccaccatt tgccaataga
     1141 tgtcatagga acgaatcctc gacacccgcc atattactaa tacccaaatg atgatcagat
     1201 taatcttgct ggaatttagc attccagccc gaaaacgcct gcctttcccg cttacgtgct
     1261 aggatgacga cgctggttat cggcatgtcg agaagacccc atctttatcg gcaggtgagc
     1321 aggctctcag tgagccccgt atccagcttg acttcgcaca tcacaaaaac acagcgccgg
     1381 tatgaatgta gacaaagcca ttattgaaga ggcccccaag aaggcgagcc aattacgctc
     1441 ttctcaggtc tacaaagaca atttggaatt ttggcatcag gcttggaata tgtgcaccaa
     1501 gccttataca aagatgcccg aactggaata cctgccgtgg attcccgagc gcctgcatgc
     1561 tgcatccgca aaatccgtcc ttgatttggg atgcggaagc ggctggcttg ccgtttacct
     1621 gtcgcgtgaa ggcttccgtg ttacgggtat tgatgtcgcc gaccacgccc tcgagctggg
     1681 aaagcagtgg gcgcagatgg aaaacctgga gataagattc gacgtcgggg acttgggcga
     1741 cctgccctac tcggacaata gcttcgatgc cgttgttgcc aattcgatta tcgaacactt
     1801 gcctttttct ctggctcttg tggcaatgac aaaactcaag tcagtaatgc gcagcggcgg
     1861 gacatttatt ggttgcttcg acaaagtagg cacaggaccc ggagaatatt tcaagctcga
     1921 agacggcacc caggtctaca ctgacaaatc aagaaagggc atgatgctgc gctatttccc
     1981 ggatgaagag ctgagagagc ttttgaccgg ctggaaaatt gatgagttta agactttgga
     2041 ttccggaacc agattcgtag tcgcgcacac ataagcccga tgacttcata tttggtggca
     2101 ttacctgtca aacacaatgc aaggaataaa taagagtcca aacgaagaat catgcagaga
     2161 agcgctgcag gatgccgaag cgcgcctggg aggcgacgat gcgcagctgg tgccctttct
     2221 cgatcagcta gcctacgcct accacagcac ggggaaatac gccgaagcag aggagtgtta
     2281 tcaccgggcg ataaagatca ccgaaaagaa agaaggcgcc aagtcaccac ttttgatacc
     2341 acgtctgcac aatctggcag ttctctatcg catccaggag aagttcccag aagccgagca
     2401 gatctacatt cgcaccattc tgctcacaga aaaacttccg ccggaaaaga tccttgactt
     2461 agccacgcag aaaaactttc tggctggttt gtattttgcc tgggggcgcc ttccggacgc
     2521 ggaaaatctc gttaatgact cgctggaaat ttacaagaaa acacttggcg cagagcacga
     2581 atatgcctct ttctgtttga tgggcttttg tctcatctat aaacagc
//
LOCUS       DAOQNZ010000485         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22250_length_2625_cov_3.251362, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000485 DAOQNZ010000000
VERSION     DAOQNZ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22250_length_2625_cov_3.251362"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKW73_09475"
     CDS             <1..397
                     /locus_tag="PKW73_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73558.1"
                     /translation="RNRLVNLHISGANIAAGELKSFLELPGLRSLTIVGGRIAAGEID
                     RMVACRSSSLTYLNIEFDETSLPYFKPASIAGLTNLKDLRLLFSKRLWDSDPHACLFI
                     VSSLRSEYPGLRVRYQTVQDGPDSIRGGD"
     gene            486..2138
                     /gene="pgm"
                     /locus_tag="PKW73_09480"
     CDS             486..2138
                     /gene="pgm"
                     /locus_tag="PKW73_09480"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HNA73559.1"
                     /translation="MLSPFAGKPAPIDMLVNVPRLVTTYFSEKPDVSIAAERVAFGTS
                     GHRGSAFKRAFNEDHILAITQAICLYRQEQGINGPLYLGIDTHALSEPAFATAMEVLA
                     ANEVNVMVDSQLGFTPTPAISLAILTYNRGRKSGLADGIVITPSHNPPQDGGFKYNPA
                     NGGPADTNITAWVEQKANEFLANKLQGVKRVSFRQAMAASTTHKFDFVDLYVKELKSV
                     VDLDAIASSGLKAGVDPLGGAGVAYWERLAAEHKLPIEVVNHRVDPTFSFMCVDWDGK
                     IRMDCSSPYAMAGLVEMRNRFDIAFANDTDHDRHGIVTPAGLMNPNHFLAVAIDYLFQ
                     NRKQWPLQAAIGKTVVSTGLIDRVAARLNRKLVEVPVGFKWFVDGLLDGSFGFGGEES
                     AGASFLRKDGSVWTTDKDGIILALLSAEITAVTGKDPGKHYEELTKEFGTPCYARIDA
                     PATPEQKSKLKKLAAEDVKASEFAGEKITGKFTSAAGNGEAIGGLKVTTENGWFAARP
                     SGTEDVYKIYAESFKSEAHLKEIQSAAQELVTQVLAPTPQKA"
     gene            2205..>2625
                     /locus_tag="PKW73_09485"
     CDS             2205..>2625
                     /locus_tag="PKW73_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA73560.1"
                     /translation="MSCKVLIPINDQEAALYSLESAMARKWSDGAQFMLMTVVEDLAA
                     AVPASRAVHKEVLAAEQAEYMEFMKGWIERLRDSLSIVFPDTSTEVNSGEICEKILET
                     AAHWGADYIILGSHEFALTSRLALGSIASRILANAPCT"
BASE COUNT          574 a          646 c          744 g          661 t
ORIGIN      
        1 ccgcaataga cttgtaaact tgcatatttc cggtgccaat attgctgccg gtgagttgaa
       61 gtcctttctg gagttaccgg gcttgcgttc tttaactatc gtgggcggta ggattgctgc
      121 gggtgagatc gatagaatgg ttgcttgtcg cagcagtagt cttacctatc tcaatattga
      181 atttgacgaa accagtcttc cgtattttaa gcctgcctca attgccggtt tgaccaattt
      241 gaaagatttg cggctgctat tttctaagag gctttgggat agtgatcctc atgcctgtct
      301 gtttattgtt tcgagcctcc gctctgagta tccggggctg agagtgcgct atcaaactgt
      361 tcaagacggc cctgactcta ttaggggggg tgattgagaa ttgagttggc cgggctttca
      421 agcgtttgtt attgggcgcg gatttcttta gaatagagcc gccttggatg ttcaggagat
      481 ataaaatgct cagtcccttt gctggtaagc ccgcccccat tgatatgctt gtcaatgtgc
      541 cccgcctggt gacgacctat tttagtgaga agcctgatgt ctcaattgcg gccgagcggg
      601 tagccttcgg tacttccggc catagagggt cggcattcaa gcgtgctttc aacgaggatc
      661 atatcctggc tatcactcag gccatttgtc tctatcgtca ggagcagggc atcaacggtc
      721 cgctttatct tggcatcgat acccatgccc tttccgagcc ggcctttgct acagccatgg
      781 aagtgctggc cgccaatgaa gtgaacgtca tggtggacag ccagttgggc ttcacgccga
      841 caccggctat ttctctggct attttgactt ataacagagg acgcaagagc ggtctggctg
      901 acggcatagt catcacgccc tcccacaatc ctccccagga cgggggcttc aagtacaacc
      961 cggccaacgg tggaccggcg gacaccaata tcacggcctg ggtggagcag aaggccaacg
     1021 agtttctggc caacaagttg caaggtgtca agcgcgttag tttcaggcag gcgatggctg
     1081 cttccaccac tcacaagttc gactttgtcg atctctacgt gaaagagctt aagagcgtgg
     1141 tggatcttga tgccattgcc tcttccggtt tgaaagccgg cgtagatccc cttggtggcg
     1201 ccggtgtggc ctactgggag cgtcttgcgg ctgaacacaa gttgcccatc gaggtggtga
     1261 accatcgcgt ggatcctact ttctctttca tgtgcgtgga ttgggacggc aagatccgca
     1321 tggattgctc ctcgccttac gctatggccg ggctggtgga gatgcgcaat cgctttgaca
     1381 ttgcttttgc caacgatacc gaccacgatc gccacggcat cgttacgcct gccggtttga
     1441 tgaatccaaa ccatttcctg gctgtggcca ttgattatct ctttcaaaat cgcaaacagt
     1501 ggccgctcca ggcggcaatc ggcaaaactg ttgtcagcac aggcttgatt gatcgtgtgg
     1561 cggcgcgtct taaccgcaag ctggtagaag tgccggtggg attcaagtgg tttgtggacg
     1621 gcttgctgga cggttccttt ggcttcggcg gtgaagagag cgccggggca tccttcttgc
     1681 gcaaagacgg ctcggtctgg acaacagaca aggacggcat tattctggcc cttttgtctg
     1741 ccgaaattac ggctgtaaca ggtaaggatc ccggcaagca ttacgaggag ttgactaaag
     1801 agttcggtac accctgttac gctcgcatcg atgcgccggc tacacctgag cagaagtcta
     1861 aactgaaaaa gttggcggct gaagatgtga aagcttccga gtttgccggt gagaagataa
     1921 caggcaaatt caccagtgcc gctggaaacg gtgaggctat cggcggactg aaagtgacaa
     1981 cggaaaacgg ctggtttgcc gctcgacctt ccggtaccga ggatgtgtac aagatctatg
     2041 cagagagttt caaaagtgag gcccacctga aagagattca gagtgccgcc caggaactgg
     2101 taacacaagt gctggcgccg acaccgcaaa aggcatagag agtcgaatcc tacagtgctc
     2161 tggtgtttaa ggcttcaacc tgaaagtttt gagggagaga ataaatgtcc tgtaaagtgc
     2221 tcattccaat aaacgaccag gaagcggccc tctattccct tgaatctgca atggccagga
     2281 aatggtctga tggcgcccag ttcatgctga tgactgtggt ggaagatctg gcggcggccg
     2341 tgccggccag ccgggctgtg cataaagagg tgttggcggc tgagcaggct gaatacatgg
     2401 agttcatgaa gggctggatc gagcggcttc gagactcgct atccatagtt tttccagaca
     2461 catcgaccga ggtaaattcg ggcgagattt gcgaaaagat cctggaaact gccgctcact
     2521 ggggcgctga ttatattatt ctcggctccc atgagtttgc tcttaccagt cgtctggccc
     2581 ttggcagtat tgcttctcgc attcttgcaa acgctccttg tacgg
//
LOCUS       DAOQNZ010000486         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22326_length_2620_cov_5.191813, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000486 DAOQNZ010000000
VERSION     DAOQNZ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22326_length_2620_cov_5.191813"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PKW73_09490"
     CDS             complement(<1..909)
                     /locus_tag="PKW73_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73561.1"
                     /translation="MDNFRRRKARGSVAETPLLMLVLFAIFAFPLIDLISLALAYGTV
                     WFVAFQSASVASTQTDFKSSLSALVRKASDFNGNGFTNMLKMTPIAGYNATGTDLYIE
                     AVDFMDASKSETIGPNKPVPPPVDLTNRFYEISAESTYQVKPFIDLGSVPLLSYVQGL
                     GSPITFTAKVKRCAEFPQGLVHGPASPGSTPASAAQPAPTTSFASPISVASQATEPWK
                     RPKLYEEIEASGQKIVDHTVIQVPANNSAWTYSGIDVAPGQQVWIDFRADGVWGDPNI
                     WAAKNLTADGTLSGSINNPQTNGGYPV"
     gene            complement(1151..>2620)
                     /locus_tag="PKW73_09495"
     CDS             complement(1151..>2620)
                     /locus_tag="PKW73_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73562.1"
                     /translation="AQESFLQALSILEKQAEPDAEMTSICARALLEIGQCLPVRDALK
                     AIIDGKSDLHQNAMVRYLYAQALTGSGDKTAAMAQLNRALELDPNSYEANLARASLNL
                     ALNQQTRARTDAQKAVELNSKSMIGYYYLAQAYLNNNEVLKARDAINQSLIIAGKKPL
                     PEAVALSAQIAIRQGDLARSAAEAALAVKLGLEGKSLGLMPLRQITTVDNVTGPLKTV
                     IARPHFLIYGSINRERLEYYGNLAEAFITYVDDNLIKVKGNFPRALFIFPDKLSARAF
                     LTDKMGFKNKVHGVYLTAQNAVVTYDGAGLGTIMHELMHTVLGEYKNLEFWAEEGIPC
                     LFDRCYGYLIAEKDDRQSMKIALGFPEEWHAKWMGGKTRAQLTLADILKHAAHTNADF
                     ENEQRQLALLLCKQGKLKTYLSLIETANKKRAATYLEAALDKPAAQIEALFQEYMQAL
                     EKENDRIRQLPETVIFDDKAEFLKFQKEHETILQPFLLK"
BASE COUNT          557 a          622 c          733 g          708 t
ORIGIN      
        1 gaccgggtag cctccgttag tctgcgggtt attgatcgag ccggagagag taccatcggc
       61 ggttaggttt tttgctgccc aaatgttggg atcaccccag actccgtctg ctctgaagtc
      121 aatccagact tgctgtccag gtgcaacgtc gatgccgcta tatgtccatg ccgaattatt
      181 ggccggtact tgaataacgg tgtggtcaac aatcttttgt ccggaagctt caatttcttc
      241 gtagagttta ggtcgcttcc agggctcggt ggcctgactg gctacggaaa tcgggctggc
      301 aaaggatgta gtaggcgctg gttgggcggc gctggccggc gtgctgcccg ggcttgcagg
      361 accatgaacc agcccttgtg gaaactccgc acagcgcttc accttggcag taaaggtaat
      421 gggacttccc aagccctgaa catagctcaa aagaggcaca gagcctaagt cgatgaaggg
      481 tttcacctga taggtcgact ctgccgagat ttcgtaaaag cggttagtaa gatccaccgg
      541 cggtggaaca ggtttattcg gaccaatagt ttcggatttg ctggcatcca taaagtcaac
      601 ggcttcaata tagaggtctg tgccggttgc gttgtagcct gcaatcggcg tcatttttag
      661 catgttggta aagccgttgc cgttaaagtc ggaagccttt ctcacgagag cggacaatga
      721 gcttttgaag tcggtttggg ttgatgccac tgatgctgac tggaaggcca caaaccagac
      781 tgtgccatag gccagagcca gtgagataag atcgatgagg ggaaaggcaa agatggcaaa
      841 gagaacaagc atgagtaggg gcgtctcggc tacacttcct cttgccttac gccttcgaaa
      901 gttatccatc acttcttatc ccccaagtaa tcagtcgtga tgccggtgtg ttaattcgac
      961 catacccgat tgtaaggttg tgtaaacatg ctctagaggt ttgtccagtc cgcttcttga
     1021 gtagtctgac cctgccaccg attggctgga cagagttaat actctccatt ggaatagcca
     1081 cttttccacg gctcgaggcg cttactcaag aaagtttttc catgcagttt ctatgcctta
     1141 agcagtgccc ttacttgagg agaaatggct gcaaaatggt ttcatgctct ttctgaaatt
     1201 ttaaaaattc tgccttgtcg tcgaagatta cagtttccgg cagctgcctg atgcggtcgt
     1261 tttccttttc gagggcttgc atatactctt gaaagagagc ttcaatttgt gctgccggtt
     1321 tatccagggc tgcttccaga taggtggcgg cacgtttctt attggctgtt tcgataagcg
     1381 aaagataggt tttcagcttg ccttgtttgc agagcaaaag agccagttgc ctctgctcat
     1441 tttcaaagtc ggcgttggta tgggcggcgt gtttgagtat gtcggccaag gtgagctggg
     1501 ctctggtttt gccgcccatc catttggcat gccactcttc cgggaagccc agggcgattt
     1561 tcatgctctg acgatcgtct ttctcggcaa taaggtagcc gtagcaccgg tcgaacaggc
     1621 atggaatgcc ctcttctgcc cagaattcga gatttttgta ttcaccaagc acggtgtgca
     1681 tcagttcatg catgatggtg cccagaccgg caccgtcata ggtaactacg gcattttggg
     1741 ccgtcagata aaccccgtgt accttgttct taaagcccat tttgtccgtc aggaaggcgc
     1801 gggctgagag tttatccgga aaaataaaga gggctctggg gaagttgcct tttaccttga
     1861 tgagattgtc gtccacatag gtgatgaagg cttcggccaa attgccgtag tactcgaggc
     1921 gttcacggtt tatggagcca taaatgagga agtgcggtct ggcaatgacg gttttgaggg
     1981 ggccggtgac gttgtctacc gtggttatct gacgaagagg catgagaccg agagacttgc
     2041 cttccagtcc aagctttacg gcaagggccg cttccgctgc tgatctggcc agatcccctt
     2101 gcctgatggc aatctgggcg ctcagcgcca cggcttccgg caagggcttt ttaccggcaa
     2161 tgatgaggct ttgattgatg gcatctctgg ctttcagcac ttcgttgtta tttaagtaag
     2221 cctgggctag atagtagtag ccgatcatgc ttttgctgtt cagctccacg gctttctggg
     2281 catccgttct ggctcgggtc tgttggttta aggccaggtt gaggcttgcc cgggcaagat
     2341 ttgcctcgta actatttggg tcaagttcca aggcgcgatt gagctgggcc atggctgccg
     2401 ttttatcgcc tgagccggta agagcctggg cgtaaagata gcgaaccatc gcgttttggt
     2461 gcaagtcgga cttgccgtcg attatggctt tcagtgcgtc tctcacaggt aaacactgtc
     2521 ctatctccaa aagagcacgg gcgcagatgg aagtcatctc agcgtccggt tcggcttgtt
     2581 tttcaagaat gctcagggcc tgcaagaagc tctcctgggc
//
LOCUS       DAOQNZ010000487         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22331_length_2620_cov_3.564522, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000487 DAOQNZ010000000
VERSION     DAOQNZ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22331_length_2620_cov_3.564522"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PKW73_09500"
     CDS             complement(<1..1395)
                     /locus_tag="PKW73_09500"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA73563.1"
                     /translation="MYKPSRTSQGTFKLPKLDRRVPNLEIIGQCLMEAQKMMGRTVEQ
                     PFSAGKPVYTYCLSCHYDSPGTAPLWHLHLGEGPTRDTLWSHQTADTSLIASLVSKEC
                     PADGDSRAAQSTTFGPSAQTAAISLTRIQAMNSAAGVQEGQPVQEAAGYFKKSAGERS
                     DPQGGGAIFQNAAMPQVQFQIQNAYQQGARVETKEETEKSNYQRTGEGQGSPAVGQPG
                     YGSQDNGNRPASGSYPKPPTGGLLGTPELGSTSGSRPPVSAWGISPQSGALINKEGAR
                     PEAATNPVAPRPEIKMTYGGDQAETAPYQTTTIPHSFMNPPSYTKRPPTLEGALTDMT
                     PPSLLQSMQIGKMTGVLMVISNEDMVEVYFEDGAPIHAVAPDTKGEAAVMELLTWDVG
                     KFAFYPNERTTERTVRRRMEGMLMESAPLMDQYRYLHEVGLSMDSFIIRRQGPISEPE
                     FEQRMERGARMDMQK"
     gene            1835..2452
                     /locus_tag="PKW73_09505"
     CDS             1835..2452
                     /locus_tag="PKW73_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73564.1"
                     /translation="MTINDDITRGERQTRPGAGLPDVVPESFEAEDVSEVCQSCGVSS
                     SMGRMNAESEADVTQVRPHTIGDSDAAGGSTANQERSGNSVNMSIQTGGLDFKLDTRI
                     DPNQQSIELSAKLELCLSMLAASQNRLEVAFVRIGQLEAELAVLRTIHSRSVSAACEE
                     PRAMDETAARSAAETADKTSYRSSAETSDRSTAESADKTGGSVLG"
BASE COUNT          477 a          678 c          821 g          644 t
ORIGIN      
        1 cttctgcata tccatgcgag caccgcgttc catgcgctgc tcgaactccg gttctgatat
       61 ggggccttgt ctgcgaatga tgaaagagtc catggacagc cccacttcgt gcaggtagcg
      121 gtactgatcc atgagcggtg ccgattccat tagcatgcct tccatgcggc gacgcacggt
      181 gcgctccgtg gtgcgctcgt ttggatagaa ggcgaatttg cccacgtccc aggtgagcaa
      241 ctccatgact gccgcctcgc cttttgtgtc cggcgcgacg gcgtgaatgg gagcaccatc
      301 ttcaaagtag acttccacca tgtcttcgtt ggagatgacc atcaagacgc cggtcatctt
      361 accgatttgc attgattgca agaggctggg cggcgtcatg tcggtgaggg cgccttccag
      421 ggtgggcggg cgcttggtgt aagagggcgg gttcatgaag gaatggggga tggtggtggt
      481 ctggtagggt gccgtttccg cctggtcgcc gccataggtc attttgatct cggggcgggg
      541 cgccaccggg ttggtcgcag cttcggggcg tgcaccttct ttgttgatga gggcgccgga
      601 ttgcggcgaa ataccccagg cgcttacggg cggtctggag ccgctggttg agcccagttc
      661 gggtgtgccg agtaagccgc cggtgggcgg tttgggatag ctgccgctgg ccgggcggtt
      721 gccattgtcc tggctgccgt agccaggttg cccgaccgcg ggagaaccct gaccctcacc
      781 ggtccgctgg taattgcttt tttcggtttc ctctttggtt tccactcttg caccctgttg
      841 atacgcgttt tgaatttgga actggacttg cggcatggcc gcattttgga agattgcccc
      901 acctccctgg gggtcggatc tctcgcctgc cgatttcttg aaatagcccg cagcctcctg
      961 aaccggctga ccctcctgga cgccggcagc ggaattcata gcctgaatac gagtgaggct
     1021 gatggcggca gtttgggcgg agggtccgaa ggttgtgctc tgagcggcgc ggctgtctcc
     1081 gtcggcgggg cattctttgg agacgaggct ggcaatgagg ctggtgtctg ccgtctggtg
     1141 cgaccagaga gtatcccttg tgggaccctc gcccaggtgc agatgccaga gcggggctgt
     1201 acccgggctg tcatagtggc aggagaggca ataggtgtag accggttttc cagcggagaa
     1261 gggctgctca acggtgcgcc ccatcatctt ctgggcttcc atcaggcact gtccgataat
     1321 ctccaggttg ggcacacggc ggtcaagctt gggaagcttg aaagtgccct ggctggtcct
     1381 ggatggtttg tacatgtgcg ctcgagtgta ccgatggtcg ctggtcaggg gccctgcatt
     1441 agcctcgccg tgcggtcttg aatgactcgg caggcagata taagaccctc tttaatagta
     1501 taaaaagaga gatttcctat ttgtgtttta cctgtttgca cgcccgctca aacgcaaact
     1561 ttttagtttt gtcccatcat ttgcgccgcc ttccagcaag gtgcgcttgg caactcggtt
     1621 gcagcctgcg agtctccttt ctctttgtct gataatggac ttttgtccgg ttaaggaagg
     1681 cacaggcaag cgcgccttgc ctcaaggtcg cttgttatgg cgtaggaatt ttgggcagtt
     1741 cgggtattaa tagagataat gagctagtct ttcggtccgt tctctgctca ggaacgttcg
     1801 gtaaggcatt tgagcctgtg gagcaaccgg caagttgacc atcaatgatg acattaccag
     1861 aggtgaacgt cagacccggc ctggtgcagg gttgccggat gttgtgccgg aaagtttcga
     1921 ggcggaagac gtcagtgagg tctgtcagtc ctgtggtgtg tcttcctcca tggggcgcat
     1981 gaacgcagag tctgaggcgg acgtgacaca ggtgcggccc cacacgattg gcgacagtga
     2041 tgccgccggt ggttccactg ccaatcagga gcgcagcggc aatagtgtca acatgtcgat
     2101 tcagaccggc ggtttggatt ttaagctgga cacccgcatc gatcccaatc agcaatctat
     2161 agaattgtcc gccaagctgg agttatgcct ttccatgcta gccgccagcc agaatcgcct
     2221 ggaggtggct tttgtgcgaa tcgggcagtt ggaggcggaa ctggccgttt tgcgtactat
     2281 tcatagtcgg tctgttagtg ccgcttgcga agagccgcgg gctatggatg aaactgccgc
     2341 cagaagcgct gctgaaactg ccgataaaac ttcctacaga agctccgctg aaacttctga
     2401 cagaagcacc gctgaatctg ccgataaaac gggaggaagc gtccttggct gaacgcgaag
     2461 ccctggtttt catcaatttt gagcaggctt tgcgcttcgg tcatgtggcc tgggggtttg
     2521 ccgttgatga cgatgaatat ttctacggtt ccaccgatca tttgttgaag gagccaatgt
     2581 ggtatctacc ggctctctgg cgctacagtc atgtaccgcc
//
LOCUS       DAOQNZ010000488         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22345_length_2619_cov_3.950858, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000488 DAOQNZ010000000
VERSION     DAOQNZ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22345_length_2619_cov_3.950858"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKW73_09510"
     CDS             <1..964
                     /locus_tag="PKW73_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73565.1"
                     /translation="KSLAPSLGEKSDWLKLEAMLALKQYDGVLQECARPGWKDTTTVQ
                     YFKARALLDRPKKDQADKSDAGTASDILSECIKQVPGTAMFHLQRARALQLLEKSDEA
                     ALDQARASELGLKDITPMTGPNLLYLGAYELSHDLLSPIRCSKKRKQMRDFESDEEKV
                     KALHSGAEVFSTFKSLYARHSPAAYGVYQDGARLRTGIQIASSVSWITTSASENRSAG
                     LASAGTMPFERTGWKTEIYGEKVTRSSAEEVEMEFDVKETTVKGSSRTEQISRVRWTI
                     KPNSLGLWKVVAEDSITKVSGSITSGAASGKSGASARKGPRKGK"
     gene            complement(969..1373)
                     /locus_tag="PKW73_09515"
     CDS             complement(969..1373)
                     /locus_tag="PKW73_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HNA73566.1"
                     /translation="MKEAVLTSHHLSAILPLKPEAFSFSAETLLNLLNQAVVDAGLAR
                     MADAVADFSPQGASAVIVLKESHVAVHIWPEHDAATVDIHVCDYMADNLAKARALAEL
                     LSAALSDKHDSDLWQELTVSKKTRARAPLAGK"
     gene            complement(1452..1901)
                     /locus_tag="PKW73_09520"
     CDS             complement(1452..1901)
                     /locus_tag="PKW73_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNA73567.1"
                     /translation="MDDIDLKLLALLQEDAKQKYADLAQRLNLSAPSVHARVKKMEQA
                     GIIESYGLKIDSSKIGLKLCAFVRVTTEGVGSELGKSLYRFKEVEEVHSVAGEECLLL
                     KVRTADTDSLSHFLDEIRRVKGVRKTITSIVLTTLLSRQVLPENSNV"
     gene            2082..>2619
                     /locus_tag="PKW73_09525"
     CDS             2082..>2619
                     /locus_tag="PKW73_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA73568.1"
                     /translation="MEAKFRPGKLQIALAFLSIYIIWGSTYLAIRFAIDTIPPLVMAG
                     ARFLLAGLPLYLVLRLMGTERPSPAEWRTSCVLGVLLLSVGTGGVVLSEKSVPSGLVS
                     LLVAMVPVYVAILEWMVGGFKKVSYLSGLGLLVSTLGIFILVSPSLLGGVGKVDAIGI
                     LLVMFSSFSWALGTVISRK"
BASE COUNT          560 a          635 c          755 g          669 t
ORIGIN      
        1 caagagtttg gcgccgtcac tgggtgagaa gagcgactgg cttaagctgg aggctatgct
       61 ggccctcaag caatacgacg gcgtgctcca ggaatgtgcc agacctggtt ggaaggatac
      121 aaccacggtc cagtacttta aggctcgtgc cttgctggat agacctaaga aagatcaagc
      181 tgataagagc gacgccggga cggcatccga catcctcagc gaatgtatca agcaggtgcc
      241 cggcacggcc atgtttcatt tgcagagagc gagagctttg cagttactgg agaaaagcga
      301 tgaggcggcg ctcgatcaag ccagggcctc cgaacttggt cttaaggata ttactcctat
      361 gaccgggccc aatttgctct atttaggtgc ttacgagctt tctcacgatc tcctcagccc
      421 aatccgctgt tcaaagaagc gcaagcagat gcgagatttt gaaagtgatg aagagaaggt
      481 caaagctctg cattcgggcg ctgaggtttt cagcactttt aagtctctct acgcaagaca
      541 tagtccggct gcctatgggg tctatcagga tggtgcccgc ctgcgcaccg gcattcaaat
      601 tgcttccagt gtttcctgga ttactaccag tgcttccgag aacagaagtg ccggactggc
      661 cagtgccggt accatgccct ttgagagaac cggttggaaa accgagatct atggtgagaa
      721 ggtaacgcgc tccagtgccg aggaagtgga gatggaattc gatgtgaaag agacgacggt
      781 gaagggaagt agccgcactg agcaaatttc gcgtgtgcgt tggaccataa agcctaatag
      841 tctggggctc tggaaagtgg tggcggagga ttccattacc aaggtttccg gcagtatcac
      901 cagtggtgcc gcctctggaa agagcggtgc ttccgcccgg aaaggacctc gtaagggaaa
      961 atagacgatt atttgccggc tagaggcgca cgcgctctcg ttttcttcga tacagtcaac
     1021 tcttgccaga ggtcgctatc gtgcttgtct gaaagggcgg cactgagaag ttctgccagg
     1081 gctctggctt tggctaagtt gtcggccata tagtcgcaaa cgtgaatatc aacggtggcg
     1141 gcatcgtgtt cgggccagat atgtaccgct acatgggatt ctttcaaaac gatgacggct
     1201 gatgccccct gggggctgaa gtcggctacg gcgtcggcca tgcgggccag accggcatcc
     1261 accacggcct ggttgagcaa attgagcagt gtttcggcgc tgaagctgaa ggcttccggc
     1321 ttgagtggca gaatggctga gaggtgatgg gaggtgagaa ctgcttcttt cattgaatcc
     1381 ggcccttgag agtgccttac aaactcgata atagcccaga agcatagcta aaccccggga
     1441 atttatttca tctaaacatt gctattctcg ggcaagacct gccgcgaaag cagggtggtc
     1501 agaacaatgc tggtgatggt cttgcgcacg cctttgacgc ggcgaatctc atcgaggaaa
     1561 tgcgaaagac tgtccgtgtc ggcggtacgt accttaagaa gaaggcattc ttccccggcc
     1621 acggagtgta cctcttctac ttccttgaag cggtaaagtg atttccccag ttccgatcct
     1681 actccttccg ttgttacccg cacgaaggcg cagagcttga gacctatctt gcttgagtct
     1741 attttcagcc cgtaactttc aatgatgccg gcctgttcca tctttttgac gcgggcgtgt
     1801 acagagggag ctgacaaatt gagtctctgg gccagatctg catatttctg tttagcgtct
     1861 tcttgcaaaa gggctaatag ttttaggtca atgtcgtcca tggttttgat ccggttaggt
     1921 ggttttgcca aatgctatta gaaagtttag catattagcg agtttggggt tgcgccccaa
     1981 tgtggttagg cgcattatgt atatagcctg actcaattag ctcattcctc cttcgggcgc
     2041 acttgaggaa ggctgaattc gcttaagaaa ggtagcacgt catggaagcc aaattcagac
     2101 caggaaaatt gcaaatagcc ttagcttttc tctccatcta tatcatctgg gggtcgacct
     2161 acctggctat tcgctttgcc atcgatacta ttcctcctct ggttatggcc ggtgcccgct
     2221 ttctcctggc cggtctgccg ctctatctgg tcttgcgcct aatgggtact gagcggccca
     2281 gcccggccga gtggcgcacc tcctgtgtgc tcggcgtttt gcttttgagc gtgggcacgg
     2341 gcggtgtggt tctctcggag aagtcggtgc cttccggtct ggtcagcctg cttgtggcca
     2401 tggtgccggt ttatgtggca attctggaat ggatggtggg cggcttcaag aaggtcagct
     2461 atttatcggg gcttgggctt ctagtgagta ctctcggtat cttcattctc gtctcgccat
     2521 cgcttctggg aggggtgggt aaggttgacg ctattggcat tctgcttgtg atgttcagct
     2581 cctttagctg ggccctggga acggtcattt cccggaagt
//
LOCUS       DAOQNZ010000489         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22446_length_2613_cov_3.443706, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000489 DAOQNZ010000000
VERSION     DAOQNZ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22446_length_2613_cov_3.443706"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1069
                     /locus_tag="PKW73_09530"
     CDS             326..1069
                     /locus_tag="PKW73_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73569.1"
                     /translation="MARTIVICLIFIVGAGIFCQVLISKGLMEDPFKGNLDSSVIDAE
                     SLEKVPLEPFILPGSQSKASFPGKPHRASNDRKLFPAYAEFGQTYFLADKTRQFSMAE
                     FALPSLDLSAQPNGGDNGLPTAESIGNVFGGTLVSERGAIGRDGVASTASAAADARLV
                     ETALAQFTERWVREYASSLDGKKVICKNRIFYGREITGTMKDQVQKYKLRVFCNFNRT
                     SMVMAAVIGSDKDIKSPVTQKFLDSVELW"
     gene            1128..1805
                     /locus_tag="PKW73_09535"
     CDS             1128..1805
                     /locus_tag="PKW73_09535"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HNA73570.1"
                     /translation="MNQQEGKSPDMFNISDGSPQVIDLAQEHIETFIGEIDVVLHETV
                     SDHLHVDVHVIPATEDRPFLTLVTTGMSDLPMNVPEELDEYKYAELLICLPPDWPLSM
                     EAFQDEKNYWPVRWLKTLARYPYNADTWLGMNHTIPLGDPPEPVCEGSQMIGFMLVPP
                     IFFDEEFGELKVSDEKTVHFYQLLPLHADEMDLKVEEGADALWEKFENNVPIGDLLDP
                     KRKSACA"
     gene            2165..>2613
                     /locus_tag="PKW73_09540"
     CDS             2165..>2613
                     /locus_tag="PKW73_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73571.1"
                     /translation="MAQKLNLKCFAEQRDLITPNGKLAVLCIDMDGTTIECQPYFDEA
                     FEEFALLMSLLGVPHEESTNALKRIYYGSMPHRGFERHRFPEALVETYEDLRKRFNLT
                     QVPDQQAEHFRSLLKGIGSAPFFRRPKLFTDCIQVLTQAHERFLLVAV"
BASE COUNT          658 a          717 c          633 g          605 t
ORIGIN      
        1 ccagagagag aaccacttga agatgccagt gatggggacg cgtttctatt cgaaatatgc
       61 gatacaaaac acggaacaaa ggtaatttca agaaccaaca agaacgagtt ctccgaactt
      121 gtatccgaca taaaagtgct gataggaaac acaaatttgc gattgtggac cgatcagcgg
      181 ggccgaacgc cgacgcccca ggaaaagcaa aggtcaacac aatagcaagc ttttctgaaa
      241 taagaagcgg caaccaggga tcaaatttcc agagtctctc ttcagagaaa atgggataca
      301 cttctccctg gaaaggggag agggcatggc caggaccata gtaatttgct tgatatttat
      361 cgttggggcc ggcatttttt gccaggtgct catctcgaag ggactgatgg aagacccctt
      421 caaaggcaac ctggacagca gcgttatcga tgcagaaagt ctggagaaag taccattaga
      481 acctttcatt ttgccgggca gtcagagcaa agcttccttc cccggcaaac cgcaccgtgc
      541 cagcaacgat cgtaagctct tcccggctta tgctgagttc ggtcagacct attttctggc
      601 cgacaaaacc agacagtttt ccatggctga atttgcgctt ccctctctgg atctaagcgc
      661 tcagccaaac ggcggtgata acggcttacc aactgccgaa tcaatcggca atgtcttcgg
      721 cggcaccctg gtcagtgagc gcggcgccat cggtcgcgac ggcgtagcat caactgcttc
      781 agccgctgcc gatgcgcgtc tggtagaaac agccctggcc caatttaccg aaagatgggt
      841 gcgggagtat gcctcatccc tggacggtaa gaaggtaatc tgcaaaaacc gtatctttta
      901 cggtcgtgaa atcaccggca ccatgaaaga ccaggtgcaa aaatacaagc tgagagtttt
      961 ttgcaatttt aatcgaacct ccatggtcat ggctgccgtt atcggcagcg acaaagatat
     1021 caagtcgccc gtcacccaga aatttctcga ttccgttgaa ctctggtagc taagtcgctt
     1081 gagttttccg ggtataactt caaggtctac cgcaagggtg aatttcgatg aaccaacaag
     1141 agggcaaaag ccctgatatg ttcaacattt cagacggttc cccccaggtt attgacctgg
     1201 cacaagagca catagaaact ttcatcggag aaatcgatgt tgttttgcat gaaacagtct
     1261 ccgatcatct gcacgtggat gttcatgtaa ttccggctac ggaagatcgc ccttttctca
     1321 ccctggttac caccggcatg agcgacctgc ccatgaacgt gccggaggaa ctggatgagt
     1381 acaaatacgc cgaactgctc atctgcttgc cgccggactg gcctttgtcc atggaagcct
     1441 ttcaggacga aaaaaactac tggccggtca ggtggctgaa gaccctggct cgctatccct
     1501 acaacgccga tacctggctg ggcatgaatc atacaattcc cctgggcgac ccgccggagc
     1561 ccgtctgtga aggctctcag atgatcggat tcatgctggt tccgccgatt ttctttgacg
     1621 aagaatttgg tgaactaaag gtctccgacg aaaaaaccgt gcacttctat caactgctgc
     1681 ctcttcatgc cgacgaaatg gacctgaaag tggaggaagg cgccgacgcg ctctgggaaa
     1741 aatttgaaaa caatgtgcct atcggagatt tgctcgatcc caagcgcaag agcgcttgcg
     1801 cttgacatcc acctttgcga aaggtcttca agctagcgaa gttcaactag cctcttataa
     1861 cttgcttcat cgactaaata gtcgttcata aattcttctt tgattcgtcg ttctctttac
     1921 tggcacagtt cagaaccatt agggatcttg gtgtaactaa attacttttc ttacttgaga
     1981 gccctttcga accgatagcc acccccgctc caatctcatc ctcgactgtt ccgagaccct
     2041 cagcaacgct gattcttctc ggctggatga acttgctccg gaaacgccaa tccaacgaca
     2101 gagccctcaa ttcaaccgcg tacgacgctc ctgtgacgtc gacgcctaac aacgtgaggt
     2161 gtgtatggca caaaaactca atcttaagtg tttcgccgag cagcgcgatc tgatcacccc
     2221 caacggcaag ttggctgtgc tctgcatcga catggacgga accaccatcg aatgtcagcc
     2281 gtatttcgac gaagccttcg aggagttcgc cctgctcatg agtcttctgg gtgttcccca
     2341 tgaagagtcg accaacgctc tcaagcgtat ttactacggc agcatgccgc atcgtggttt
     2401 cgaacgccat cgcttcccgg aagccctggt cgagacctac gaagacctgc gcaagcgctt
     2461 caacctgacc caggttcccg accagcaggc cgagcatttc cgctcgctgc tgaagggcat
     2521 cggctctgcg ccgttcttcc gtcgccccaa gctcttcacc gactgcatcc aggtgctcac
     2581 ccaagctcat gagcgcttcc tgcttgtggc ggt
//
LOCUS       DAOQNZ010000490         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22477_length_2611_cov_4.929577, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000490 DAOQNZ010000000
VERSION     DAOQNZ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22477_length_2611_cov_4.929577"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKW73_09545"
     CDS             complement(<1..245)
                     /locus_tag="PKW73_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73572.1"
                     /translation="MSKNALVFLATIPASVEELAITVKKARVRFVSGKGKDLTVKVSD
                     PEQWRFVAEAGVFLQKAGKGGAASNLNVMGDVVVGKGG"
     gene            1460..2110
                     /locus_tag="PKW73_09550"
     CDS             1460..2110
                     /locus_tag="PKW73_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73573.1"
                     /translation="MFNSKDKAATVSLATLSTCLAQYPFKDLTTLPGFEPVQCELELL
                     TKYNAYKELLIRFVEEKEANNQAFSQLRREDQQREANHPSEGDKENGRKRLSYASSRL
                     TLELIYDSLLDSALRLQEDLTNPKDYDELPGYDSWRDDRQLLYKANRIMAAGLLGYFL
                     KIKAWMCSVSSRPKHEGTINGGSLTLPNEVFRLLTADGRDFLYSNYVHEKYVRPAE"
     gene            2192..>2611
                     /locus_tag="PKW73_09555"
     CDS             2192..>2611
                     /locus_tag="PKW73_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73574.1"
                     /translation="MSETQTEQLPVLDSSTQLTSAIAACNQARVLAEQVLQQKLDANQ
                     AAYIQKLAEPCNELNRLEAKAKANCNAETDPIEAQIAELERNRQRFERERLEKLKPLQ
                     NTLDLLKQLKKDLKALPTNLAAAGLKVQPQQISEADIK"
BASE COUNT          805 a          751 c          544 g          511 t
ORIGIN      
        1 ccgcccttgc cgacaacgac atcgcccatg acgttgagat tggaagccgc accacccttt
       61 cctgccttct gcagaaaaac accggcttcc gctacaaagc gccattgctc cgggtcactg
      121 actttcacag tcaaatcttt gcctttgccc gacacgaatc gcaccctggc tttcttgacc
      181 gtgattgcaa gttcttcaac ggaagcggga atggtagcaa ggaaaacgag agcatttttg
      241 ctcataatga tcacctgtat gatctgaacg tgcttaaaag ggaaaccgga tcaaccgact
      301 tccacctgat cgaagactca gtcgctcact ctgaagttaa atgcactggg tccaagatct
      361 ctccactttg cctctaatac gagccccggc ctacaaacaa cagcgcatat tgcttactgc
      421 tcaattaata attccatttg tggcaatgcg ctgatagctg tgactggctt aaaaaggctc
      481 cggaaaagag aaagtgaaga taccaaagcc tatgaaatct catgagaata aggaagatca
      541 aaatatgatc tatagaaaca ccaacaatgg ctccaatagc tacaaacagg ctaaaactca
      601 gtaattgctc tctgcctatg caaatgaaag aaaaccctga gcctgaacaa gagaacatta
      661 cacgcctcta accgtcccgc caagcagccc caaatcgtcc tttccgtaat cacagttccg
      721 ccacataggc tcaaggacac atacaacgac ttccccaatc tctggcattg caaatggccg
      781 gtttcaactt tcccaagcat ctaaatcggc atcatgccct gattcccaga cgactctcaa
      841 tctcgaccgg aaacagccac agtcagaaca gtttcaaacc aaatcgcgag acactaccaa
      901 atattttccc tgcaatcagc cagaagcctt cacctgtgag cctttagcta aacaggcaaa
      961 gataagcccg cgctaattta ctttctggga ggactacgat gcctgctcac tgccaatctg
     1021 ccgtccgctt gccatagaca cccccataaa ccacaccgca tcaaactcca gaccatagac
     1081 agcggcagca aatacctgta acagcaactc ccggattcac ttgcagcaga gatacagtct
     1141 ctagtacgac agaaagtgaa tttcaattgc atcttcctca aaggataaat acctaaacca
     1201 accttgtccc ggccgccacg gaaagcaaaa ctctctcgcc ttgaacgaga gcggcaaaga
     1261 ctgccgaccg acggacaagt gcaattgttc attcatggct aaaagcaata gcaatgctaa
     1321 tcccaaacca atagtgcacc gatgaggcga ctgaccgatc cgtgtcatgc cgctacttcg
     1381 ctgcgccgat gccggtgtct gagcctggac acgagacccg gcatcaatca aatcatccag
     1441 gtcgatacgg agacaaacca tgttcaattc aaaagacaaa gccgccactg tttctctggc
     1501 aacactctca acctgcctgg ctcagtatcc cttcaaagac ctgaccacac tgcccggctt
     1561 tgagccggtt cagtgcgaac tggagcttct gaccaagtac aacgcctaca aggaacttct
     1621 aatacgcttc gtagaagaaa aagaagccaa caatcaggcc ttttcgcaat tgcgccggga
     1681 ggaccagcaa agggaagcca atcacccaag tgagggcgac aaggaaaacg gtagaaaacg
     1741 attgagttac gccagttcaa gactgaccct ggaactcatc tacgactcac tactagattc
     1801 cgcactgcga ctgcaggaag atctaaccaa tccgaaagac tacgacgaac tgcctggcta
     1861 tgacagttgg agagacgata ggcaactgct ctacaaagcc aatcgaataa tggcggcagg
     1921 ccttctcggc tacttcctaa aaatcaaagc ctggatgtgc tcggtaagtt ctcgcccgaa
     1981 acatgaagga acaatcaacg gcggctcact caccttgccg aatgaagtct tcagactgct
     2041 taccgcagat ggcagggatt tcctgtacag caactatgtg cacgaaaaat acgtgcgtcc
     2101 ggctgaatag ccaacatctg cccataccga cgactgtgcc atctgtatca ggcacagtcg
     2161 ccatcattta acagaaaaac aaggaactcc tatgtcagaa acgcaaacag aacaactacc
     2221 ggtgctagac tcatccaccc agctcacctc agcgatagcg gcctgcaatc aagccagggt
     2281 gcttgccgaa caggtgctac agcaaaaact ggatgccaac caggcagctt acatacaaaa
     2341 gctagccgaa ccctgcaacg aactaaaccg tctcgaggca aaagccaaag cgaactgcaa
     2401 tgctgaaaca gacccgatcg aagcccaaat tgctgaactc gaaagaaatc ggcaacgctt
     2461 tgaaagagag cggctggaaa aactgaagcc cctgcaaaac accctggatt tgctgaagca
     2521 gctgaagaaa gacctcaaag ctctgccgac aaacctggcc gccgccggtc tgaaagtgca
     2581 gccgcaacaa atatctgaag ccgacatcaa g
//
LOCUS       DAOQNZ010000491         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22501_length_2610_cov_3.380822, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000491 DAOQNZ010000000
VERSION     DAOQNZ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22501_length_2610_cov_3.380822"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1446)
                     /locus_tag="PKW73_09560"
     CDS             complement(31..1446)
                     /locus_tag="PKW73_09560"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA73575.1"
                     /translation="MVDIRRRFSLRRSCIALSLLFLPVVSVLSLSACGEHSKPVEEKK
                     PEKSSGPKIIPLTEETAKRIDFQTETVTSRAVVVPLHLTGRIEPDYGCAVDVSARIAG
                     RITEIKVKPGEMVSRGQLLAMVDSPPVTDLQGELVEAKSKLAIAEAHAERERQIYEEH
                     LERPKGLLDARALMQNTRVQAELAELEYQRLEGLYREKIAATKDYLAAKAALAKAKVD
                     FEQASTALAREEQLYKNRALMKRDYQLALAEVTRMKQHLNTIVKRLDFLGADHTMTQR
                     VLKTGDINGLVRIVSPITGILSKYEVGVGEMVQPDLSMFRIIDLKTVQASADLPEVDL
                     ERVKLGDTVRIHVPSYPDRKFQGKISFISSDVNPLTRTVPIRARLVNSTGLLKANMYA
                     EIDLEGASRNFLACPKAAIQEREGRKVVFVKHPDGFEERAVKLGVIGEQYIEVQSGLS
                     DGDVVATQGSLMLKTELSYQH"
     gene            1730..2023
                     /locus_tag="PKW73_09565"
     CDS             1730..2023
                     /locus_tag="PKW73_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNA73576.1"
                     /translation="MRLDKWLKVSRLIKRRTVAQMACDQGRVYVNDKTAKSAVELKVG
                     DKVHLELGARAITVTVLAVPLKAVAAQECSSLYEITEEIRRPPEILEWLDEQV"
     gene            complement(2097..>2610)
                     /locus_tag="PKW73_09570"
     CDS             complement(2097..>2610)
                     /locus_tag="PKW73_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA73577.1"
                     /translation="IADQARTIRIPVHMVETINKLKKVTRKLQQDKGRKPTEEEIAET
                     MEISITKLRDIIKVAQEPVSLETPIGKEEDSKLGDFIEDREAETPAASVTQDLLREDI
                     IEVMASLSPRERDVLRLRFGLDDGRQRTLEEVGQLFGVTRERIRQIEAKALRKLRHPN
                     RSRRLREYID"
BASE COUNT          593 a          715 c          683 g          619 t
ORIGIN      
        1 aatcagtttg atcataattg cccttactca ctagtgctga tagctgagct cggtcttgag
       61 catcaaggag ccctgggtgg ctacgacgtc gccgtcagac aagcctgact gcacttcgat
      121 gtattgctca ccaatcacgc ccagcttgac agcacgttct tcgaagccgt ccggatgttt
      181 gacaaatacc actttgcgcc cctctcgctc ctgaatagcc gctttgggac aggccaggaa
      241 attgcgggag gctccctcca ggtcaatttc agcatacatg ttagccttca aaagtcctgt
      301 ggaattaacc aggcgagccc gaatcggcac agtgcgcgtg agcggattta catcggagct
      361 gatgaaacta attttgccct gaaacttacg atcaggataa ctgggcacgt ggatccgcac
      421 cgtgtcgccg agttttactc gctccagatc aacttccggc aagtcggcac tggcttgtac
      481 ggtcttcaaa tcaatgattc ggaacatgga aagatcaggt tgaaccatct caccgactcc
      541 cacttcgtac ttgctcagta tgccggtgat aggcgagaca atgcgcacca gaccattaat
      601 atcaccggtt ttgagcacac gctgcgtcat ggtgtgatcg gcgccgagga agtccaaccg
      661 cttgacaata gtattcagat gctgcttcat cctggtcact tcagccagtg caagctgata
      721 gtcacgtttc atgagagcac ggttcttgta gagctgttcc tcccgggcca gggccgtgct
      781 ggcctgctca aaatccacct tagctttggc aagggcggcc ttggctgcca ggtaatcctt
      841 ggtagcggca atcttttccc ggtataaccc ttccaaccgc tgatattcaa gctcagccag
      901 ctcagcctgc actcttgtgt tttgcatgag agctcgagca tcgagcaagc ctttgggccg
      961 ctccaggtgc tcctcatata tctgcctttc ccgctcagcg tgagcttctg caatggcgag
     1021 cttagattta gcttccacca gctcaccctg caaatccgtc acaggcgggc tatcgaccat
     1081 agccagcagt tgaccacgcg agaccatttc gcccggctta accttgattt cggtgatgcg
     1141 accggcaatt ctggcactaa cgtccacagc acagccgtag tcaggttcta ttcttccggt
     1201 caggtgtaac ggcacaacaa cggcgcggct tgtcactgtt tcagtctgaa aatcgatacg
     1261 cttggcggtt tcctcggtga gaggaataat cttgggaccg gaagactttt caggcttctt
     1321 ttcttccaca ggcttagagt gttcgccgca ggcgctcaaa gaaagtaccg aaacaaccgg
     1381 caaaaacaga agcgagaggg cgatgcagga tctccgcagg gaaaatcgcc tccggatgtc
     1441 aaccatttcg gaccgtgccg tgctatcggc gcctggtttg gttaagtagt tgttgaacaa
     1501 tgcttgttga cttgtacgca atcgtacaga ggggacccct gggtagagaa gagcatacac
     1561 ccagctaaga tatttcgcca aaattcttag tacaacactt gaaaattcgc aatgttgggg
     1621 tccgagatac cgtcccctgc cccaagaggg atggcaaacc ggtcctgccc ggagcaaaca
     1681 cccgccggag caaacacgct atagaatatt gccaaacggg aggcagacta tgagactgga
     1741 caagtggctg aaggtatcgc gcctgatcaa gaggcgcaca gtcgcccaga tggcctgtga
     1801 ccagggtcgg gtctatgtca acgacaaaac cgccaaatcg gcggtggagc tgaaggtggg
     1861 cgataaggtc catctggaac tgggcgcccg ggccattacg gtgactgtcc tggcagtccc
     1921 cctgaaagca gttgctgccc aagagtgctc ttctttatat gagattacgg aagagatccg
     1981 ccgcccgccg gaaatactgg agtggctgga cgaacaagtc tgaagcacag cccgcaacat
     2041 taccgagacc agaacagacc ggcttattgc agcactacat tcattccgag cttaatttag
     2101 tcgatgtatt cgcgcagacg gcggctacgg ttcgggtgcc gcagtttgcg taaagctttg
     2161 gcttcaatct gacgaatacg ttctcttgtt acgccgaata gctgccccac ttcctcaagg
     2221 gtacgctggc gaccgtcatc gagaccgaag cgcagacgca atacatctct ttctctggga
     2281 gaaagggatg ccattacttc aatgatgtct tcccggagca gatcctgggt aacggacgca
     2341 gcaggagtct cggcttcgcg gtcttcgata aaatcaccga gtttgctgtc ttcttcctta
     2401 ccgattggcg tctccaggga aacaggctcc tgcgccacct tgataatgtc ccgcagcttg
     2461 gtgatgctta tctccatggt ctcggcaatt tcttcttccg tgggcttacg gcccttatcc
     2521 tgttgaagct tgcgcgttac cttcttgagc ttgttgatgg tttcaaccat gtgcacgggt
     2581 atgcggatgg tgcgagcctg gtcggcaatg
//
LOCUS       DAOQNZ010000492         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22516_length_2609_cov_3.306578, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000492 DAOQNZ010000000
VERSION     DAOQNZ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22516_length_2609_cov_3.306578"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="cobA"
                     /locus_tag="PKW73_09575"
     CDS             complement(<1..830)
                     /gene="cobA"
                     /locus_tag="PKW73_09575"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNA73578.1"
                     /translation="MDKSASAKNRKYCQPNELLAKQLGVMKPLLERHTNESGEPRAYL
                     IGAGPGDIEYLTLKAIKAIGACDVLLIDELVNRQVLEFAAPDCEIIEVGKRAGKHIMP
                     QMEIEALFVERVKAGAVVGRLKGGDPFVFGRGGEEMLALKTAGIKCAIVSGVTAGIAA
                     AAAADIPVTHRGLAVSATFLTGHLSKETEEGDDSREPNWQALADSRSTLVIYMGLRQL
                     QNISSKLMSCGLSPDTPAAVIENGTTERQKIVRGKLGNIAYKVKQEKLNTPTLIIIGS
                     V"
     gene            complement(830..>2609)
                     /locus_tag="PKW73_09580"
     CDS             complement(830..>2609)
                     /locus_tag="PKW73_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA73579.1"
                     /translation="MCNGSDSAVKAEPTPVPFINLPLGVSEERLLGTLDLDKILKESK
                     KDFQPGLLAACHRGILYIDEVNLLPDHIVDLLLDTVAMGVNIVEREGFSISHPARVSL
                     IGTMNPEEGDLRPQLLDRFGLMVDVKAPSDPKLRAEIVSRRSKFESDPEGFSRLYLAS
                     QEVQRERIKKAGELLAGVQLSESMLLFASTICSDMGLASLRGDIVLGQTARTLAALEG
                     RLHVSLEDLKLAARLALPHRQRTRPGEKPGINQDRLDQLFQNATSDLEEGKEGEEQEE
                     RKQNLPPGGSHTMPGHGAATEAEQDPCLNQENEGEQCEKKNFAIGINPGQITLNIKEN
                     HRPETTSPNQAGKYEAGNNKSSLGVSRTAVLNPEPTNLAAHATIKHALVRTGGKITID
                     RQDLHQKEKVQKRKNLILFVVDCSGSMAARKRMEVVKGTVKDLLDGAYQEREEVSVIT
                     FNESRARLALQPTRSIDQAHRELACMETGGTTPLPHALKLVDDFISGNEQRDPLMVLI
                     SDGRTNVSLEGGDSFQESLALAEILGNRGLTSLVIDVESGFVKLAKARELAEKLQATY
                     LCLENLTSENLTLNVRKQLKSRQKFQ"
BASE COUNT          463 a          696 c          707 g          743 t
ORIGIN      
        1 acagagccga tgataatcag tgttggggtg ttcagttttt cttgtttgac tttgtaggcg
       61 atgttgccga gtttgccccg tactatcttt tgtctttcag tggtgccgtt ttcaataact
      121 gccgccgggg tatcagggga aagcccgcaa ctcatgagct tgctgctgat attctggagt
      181 tgcctcaaac ccatgtatat gaccagggtc gatctgctgt cggccagggc ctgccagttg
      241 ggttctctgc tgtcgtctcc ctcctctgtc tctttgctca ggtgaccggt aagaaaggtg
      301 gcgctgacgg ccagacctcg gtgtgtcacc ggtatgtcgg cggcggccgc tgccgcaata
      361 ccggctgtga caccgctgac aatggcgcac ttgatgccgg cggtttttaa tgccaacatc
      421 tcttcgccgc ctctaccgaa aacaaacggg tcgccgccct tgagtcgtcc caccaccgca
      481 ccagctttga cacgctccac gaagagcgct tcaatttcca tctggggcat gatgtgcttg
      541 ccggcacgct tgcccacttc aataatttcg cagtcagggg cggcaaattc taagacctga
      601 cggttgacca gttcgtcaat tagaagtacg tcgcaagccc ctatagcttt tatggctttg
      661 agtgtgaggt attcgatgtc gccgggaccg gctccgataa gataggccct gggttcgccg
      721 ctctcgttcg tgtgccgctc cagaagcggc ttcatgacgc cgagctgctt cgccagtagt
      781 tcatttggct ggcagtattt tctgtttttt gctgacgccg atttatccat tactgaaatt
      841 tctgcctgct cttgagttgc ttgcgcacgt ttaaagtgag gttctcactg gtgagatttt
      901 ccaggcagag ataagttgcc tgaagtttct ccgccagttc ccttgccttt gccagtttga
      961 cgaaaccgct ctctacatcg attaccagag aggtgagacc gcggttgccg agaatttctg
     1021 ccagagcgag gctctcttga aacgagtctc cgccctctaa actgacattg gttctgccgt
     1081 ctgaaatcag caccatcaag gggtctcgtt gctcattgcc ggagataaag tcatctacca
     1141 gtttgagggc gtgcggcaag ggggttgtgc cgccggtttc catacatgcc agttcccggt
     1201 gagcctggtc gatactgcgt gtcggctgca aggcgaggcg cgcccggctt tcgttgaagg
     1261 taattaccga tacttcttcc cgctcttgat aggcgccgtc cagaaggtct tttactgttc
     1321 ctttcaccac ttccatacgt ttgcgagcgg ccattgaacc ggaacagtca accacgaaga
     1381 gaattaagtt ctttctcttc tgcactttct ctttctggtg aaggtcctgg cggtcgatag
     1441 tgattttacc gccggtgcgc accagggcat gctttatagt ggcatgggcc gccaggttgg
     1501 tgggctcggg attgagcacg gcggtgcgac ttacgcccag gctacttttg ttgttgccgg
     1561 cttcgtactt accagcctga tttgggcttg tcgtctccgg tctatggttc tctttaatgt
     1621 tgagggtgat ttgtccggga tttatcccaa tagcaaagtt ttttttctcg cactgctcac
     1681 cctcgttttc ttgattgaga caggggtctt gttctgcttc cgttgctgcc ccgtgcccag
     1741 gcattgtatg acttccgcca gggggaaggt tttgcttcct ctcttcttgt tcttctcctt
     1801 cttttccttc ttctaaatct gaggtcgcgt tttgaaaaag ctggtcgagt cggtcttgat
     1861 tgatgcccgg tttctctccg ggtctagttc tttgccggtg gggcagtgcc agtctggcag
     1921 ccagcttcag gtcttccagg ctgacatgca gtcgtccttc taaggccgct agagttctag
     1981 cggtttgccc gagcacgatg tcgcccctca gactagcaag acccatgtca ctgcagatgg
     2041 tgctggcgaa gagcagcatg ctttccgata gttgtacgcc ggcaagaagt tcacctgctt
     2101 ttttgatgcg ttccctttga acttcctggc tggccaggta gagtctgctg aagccttccg
     2161 ggtcgctctc gaacttgctt ctacggctga ctatttcggc tcgcaatttg gggtcactcg
     2221 gtgccttgac gtccaccatc agtccgaatc tgtcgaggag ctgggggcgc agatctcctt
     2281 cttccgggtt catagtgccg atgagcgaaa ctctggcggg gtgcgaaatt gaaaagccct
     2341 cgcgctcgac gatgttgacg cccatggcta ctgtgtctaa aagcagatca acaatatgat
     2401 caggcagaag gttgacttcg tctatgtaga gtatgccgcg gtggcaggct gctagtagcc
     2461 cgggctgaaa atctttcttg ctttctttga gaattttatc gagatccagt gtgccaagca
     2521 gcctctcttc actcactcct agcggcaggt tgatgaaggg aacgggagtg ggctccgcct
     2581 ttacggcgct gtcacttccg ttgcacatg
//
LOCUS       DAOQNZ010000493         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22520_length_2609_cov_3.074785, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000493 DAOQNZ010000000
VERSION     DAOQNZ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22520_length_2609_cov_3.074785"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="PKW73_09585"
     CDS             complement(<1..1522)
                     /locus_tag="PKW73_09585"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73580.1"
                     /translation="MSENHSLNVSAVNVSAVEPAKRLVLSLSLSLAMLSGLAVTSLSV
                     CLPAPADAQAGAEQSEGLPIKDIKVDDSGQLVIVFATGGAFPEAPRVAELDSPRRVVM
                     DFQDAALEVGSLPSVDALNARLTGAVPGVKGIRYSMVGGARPIARIVLELDAAAPVKA
                     KVAKVEEGAVTVSLLDAAGGASGATAGGATAASDKDAYDDYYKQLLNQKEADKKASAE
                     WGPRRGSLSEVGGKVAIKPITNVSNWMGGFWGKKDQGDAAASAKKPESKAPGTNAAAE
                     PAERAASNQSAAQTKAPEQSLLSKGLGVGKLWKRGKAASPATQSQPQPAEASTAPAEA
                     PTSATAGTSPTAENADSGAAAKSASGEGGWGEWGDGNKTSQTNPAQEKPVQAEAPAPV
                     TPVESPTAAAPEPTPASETKATGAGEVGNPQTQADASVGNSDAANSETPSSPLESASG
                     ETGASETPRYKAGKLFNKAVHQHLSGNLAEAIKDYQAAVGIDPELGQAYCNLGLAYN"
     gene            1748..2251
                     /locus_tag="PKW73_09590"
     CDS             1748..2251
                     /locus_tag="PKW73_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73581.1"
                     /translation="MKLTLKTKDSRRKSASRLTLLLVLGSTAFLTTPQSGAASNCESG
                     RLCAPQTLAWFRKKQLPPEPPAPMPSPDNLVPPPSEEIYFPVRADGQPFLLNIDTTIN
                     LTDAISSFYYDPKWRGEVWAPFDQVSLIERSTRLMSTWPEYDQFSQYRIFNVINKKLE
                     KYSGFGP"
     gene            2269..>2609
                     /locus_tag="PKW73_09595"
     CDS             2269..>2609
                     /locus_tag="PKW73_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA73582.1"
                     /translation="MSSTEKDTAVASHDESCPGKRLDHVAIAVENLEEALKFYRDGLG
                     LTFLETEVVEEQGVRVAKLSTGNTHIELLEPLSPDTPVGKFIAKNGPGLHHICLGVEN
                     IVDNLALLKEQ"
BASE COUNT          580 a          768 c          712 g          549 t
ORIGIN      
        1 ggttgtaggc cagccccaga ttgcaatagg cttgacccaa ttcgggatcg ataccaacgg
       61 cagcctgata gtctttgatg gcctcggcca gatttccgga gagatgctga tgaacggctt
      121 tgttgaagag cttaccggct ttgtagcgag gagtttcgct ggcgccggtc tcgcctgagg
      181 cggattccag gggcgaactg ggagtttcgg aattggcagc atcactgttt ccgacagagg
      241 catcagcctg agtttgagga ttgccgacct cgccggcgcc tgtagccttt gtctcagagg
      301 caggtgtcgg ctccggcgca gcggcggtag gagactccac cggcgtcaca ggggcaggtg
      361 cttcagcttg aacgggcttt tcttgcgccg ggttagtctg gctcgtcttg ttgccatcgc
      421 cccattcacc ccaaccgcct tcaccagagg cgctcttggc ggcggctcca ctgtccgcat
      481 tctccgccgt gggagaagtg ccggccgtag cgctggtcgg cgcttcggcc ggagcggtgg
      541 acgcttcagc aggctgcggt tgagactgag tggcaggcga agctgcttta ccgcgcttcc
      601 agagttttcc cacacccaga cctttgctca agagtgactg ctccggggcc ttggtctggg
      661 ccgcgctctg gttggaagca gcacgttctg ccggttcggc cgcggcatta gtgccaggag
      721 ccttgctttc cggtttctta gcagaagcag cggcatcgcc ctggtctttc ttgccccaga
      781 atccacccat ccagttggaa acattggtga tgggtttgat ggctactttt ccgcctactt
      841 ccgaaagcga accacgacga ggtccccatt cggcggacgc ctttttgtcc gcttctttct
      901 gattgagcaa ttgcttgtag tagtcatcat aagcatcttt gtcggaagcg gcggtcgcac
      961 cgcctgcagt ggcaccggaa gcgccaccgg cggcgtcgag aagactgaca gtaaccgcgc
     1021 cctcttctac ttttgctact tttgccttga ccggagcggc agcatccaac tccagaacaa
     1081 tacgagcgat tggtctggcg ccccctacca tggaatagcg aattcccttg acgcccggca
     1141 ctgcccctgt cagacgagcg ttgagagcat caacggaggg caaggaaccg acttccagag
     1201 cagcatcctg aaaatccatt accactcgcc ggggtgaatc cagctcggcc acgcgaggtg
     1261 cctccggaaa ggctccgccg gtagcaaaaa cgataacgag ttggccggaa tcatccactt
     1321 ttatatcttt gataggcagc ccttcactct gttcggcacc ggcctgggca tcagctgggg
     1381 caggaagaca aacggacaag gaagttaccg ccagtcccga cagcatcgcc agggaaagag
     1441 agagggacag aaccagtcgc ttggcgggtt ccaccgcaga gacatttacc gcagagacat
     1501 ttaacgaatg attctcagac atacgagcaa aacctcgaac cagggcagaa cctatgacaa
     1561 ttggtccaaa gtgtactaaa gacccgccat agtttttagc caatccgttt aattaacgcc
     1621 gccggtacgg gtctggaatg cacccggtaa cacccgctcc aacagggcaa gagttttctg
     1681 taatgaacaa aagtactctc gactcgtata ataccggtga tgaaagttgt ctcacccgcg
     1741 gtccatcatg aagctgacgc tgaaaactaa agacagtaga aggaaaagcg catcccggct
     1801 cactcttctt ttggtcctcg gctccaccgc cttcttgact acaccccagt ctggcgccgc
     1861 ctctaattgc gaaagcggcc ggctctgcgc tccccaaacc ctggcctggt tcagaaagaa
     1921 gcagttgcca ccggagccgc cggcgcctat gccctcgccg gacaacctgg taccgcctcc
     1981 cagtgaggaa atctatttcc ctgtgcgcgc agacgggcag ccgtttctgc tcaacattga
     2041 taccaccatc aatctcaccg acgccatctc cagcttctac tacgatccca agtggcgagg
     2101 agaagtctgg gctcccttcg atcaggtttc attgattgaa cgctccacca ggctaatgtc
     2161 cacctggcct gagtacgacc agttttctca atatcgcatc ttcaacgtca ttaataaaaa
     2221 gctggaaaaa tattccggat ttggtccctg agcccgggag tataaaactt gagtagcaca
     2281 gagaaagaca ccgccgtggc aagccacgac gaaagctgcc cgggcaaacg cctcgaccac
     2341 gtggccatag ccgtggaaaa tctggaagaa gccctcaagt tttatcggga cgggctcgga
     2401 ctcaccttct tggaaacaga ggtagtggaa gagcagggcg tgcgagttgc caagctcagc
     2461 accggtaaca cccacatcga gctactggag cccctttccc cggacactcc ggtgggcaag
     2521 ttcatcgcca aaaacggtcc cggactacat catatctgcc tgggagtaga gaacatagtc
     2581 gacaacctgg cgctcttgaa agagcagaa
//
LOCUS       DAOQNZ010000494         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22543_length_2607_cov_3.648119, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000494 DAOQNZ010000000
VERSION     DAOQNZ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22543_length_2607_cov_3.648119"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PKW73_09600"
     CDS             complement(<1..578)
                     /locus_tag="PKW73_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73583.1"
                     /translation="MNSNLPHQEQPQTLPKPATPELMVVVGEPSADKHAARVLSRLKE
                     LQPDLHIFGAGGTEMEKAGVELLFNCNNFAVLGIFEVIKSLLFFYNMRQTLLKAVSER
                     KPKAVLLVDMGGFNIQFATALRKAHPKLPIYYFISPQVWASRPWRIDVLGKAITKMLT
                     IFPFEEHLYTDKKIPARFVGHPLLQDLPSPEDL"
     gene            complement(598..1332)
                     /gene="ubiE"
                     /locus_tag="PKW73_09605"
     CDS             complement(598..1332)
                     /gene="ubiE"
                     /locus_tag="PKW73_09605"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNA73584.1"
                     /translation="MSLPTIEEKAAYVHDQFERIAGGYDRANDVISMGMHRAWKMRAV
                     SQLVDVGGPGRFLDVCCGTGDLSILLARMAAPGSTVTGLDFSQNMLDIADIRAHRKLR
                     KIPPQTILDFVRGDAQNLPFERNSFDGAIISFGLRNLTDLQKGLTEMARVVRPGGKVA
                     NLDLGHCQVPVFAPVFSAYFGKVVPVLGGILQNDKKAYTYLPESLSTYPKPEKITEMF
                     ENAGLVDIQHIPLALGTVALHIGTRR"
     gene            complement(1347..1580)
                     /gene="secG"
                     /locus_tag="PKW73_09610"
     CDS             complement(1347..1580)
                     /gene="secG"
                     /locus_tag="PKW73_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNA73585.1"
                     /translation="MSAAYIALLVIGAVLAVSLIGLILLHAPKGDGMAGIGAAATVFS
                     GKRGAEAGLDRLTWIVFGLFMLDCLVVGFGFVK"
     gene            1705..>2607
                     /locus_tag="PKW73_09615"
     CDS             1705..>2607
                     /locus_tag="PKW73_09615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNA73586.1"
                     /translation="MSEKNKSGNHHKVVIVGGGTAGISVAARLMRAIGDVDIALIEPS
                     EKHYYQPLWTLVGGGICDKSESVKDEYSLIPSGVKWIKDKVSDVKPDSNLVKLASGGE
                     VHYDYLILAPGLQIDWHKIPGLTETLGKNGVCSNYSYELVDKTWEFISKFKGGNAVFT
                     QPNTPVKCGGAPQKIAYLAEEAFVNSGVRERTKVIFCSANAGIFSVKKYANTLSKVLE
                     RKQIETRFRTDLIAINGAEHKATFKHLDTGAESTIEFDMIHVTPPMSAPDFIKGSAVA
                     DQTGWVDVDKNTLQHKRYENVFALG"
BASE COUNT          614 a          660 c          730 g          603 t
ORIGIN      
        1 aggtcctcgg ggctaggcag gtcttgcagg agtggatgcc ccacgaagcg cgccggaatt
       61 ttcttgtcgg tatataagtg ttcttcaaat ggaaagatgg tcagcatctt ggttatggcc
      121 ttgcccagca catcgattcg ccaggggcgc gaggcccaga cctggggtga aataaagtaa
      181 taaattggca gcttgggatg ggctttgcgc agggccgtgg caaactgaat attgaagccg
      241 cccatatcta caaggagcac ggctttgggt ttgcgctcgc tgactgcctt gagcaaggtc
      301 tggcgcatgt tgtaaaagaa gagcagagac ttgattactt cgaagattcc caggacggca
      361 aagttattgc aattgaagag cagctctacc ccggcttttt ccatctccgt tccgccggcg
      421 ccgaagatat gaaggtcggg ttgtaactcc ttgaggcgac tcagtaccct ggcggcgtgc
      481 ttgtcagccg atggctctcc caccaccacc attagttccg gggtggcggg ctttggaagg
      541 gtttggggtt gctcctgatg aggcaggttg gaattcacct gcacctcccg ctcgaggtca
      601 gcgtcttgtg ccaatgtgta gtgccaccgt ccccagtgcc aggggaatat gctgaatatc
      661 tacaagaccg gcgttctcga acatttccgt tatcttttcc ggtttaggat aggtgctcaa
      721 agactcgggc aaataggtgt aggctttttt gtcgttttgc aagatgccgc ccagtaccgg
      781 taccactttt ccaaagtagg ccgagaatac gggcgcaaat accggtacct ggcagtgacc
      841 aaggtcaagg tttgctactt tgccgccggg gcgaaccaca cgggccattt ccgtgagacc
      901 cttttgcagg tcggtgaggt ttctcagccc gaagctgata atggcgccgt caaaagaatt
      961 tctctcgaaa ggcaagtttt gagcgtcgcc ccgcacgaag tcgagaatgg tttggggcgg
     1021 tatttttcgc agtttacgat gggctctaat gtcagcaata tcgagcatgt tttgggaaaa
     1081 atcgaggcct gtcacggtac tacctggggc agccatgcgg gccaggagta tggagaggtc
     1141 gcctgtgccg cagcaaacat cgaggaagcg accgggaccg cccacgtcaa ccagttgact
     1201 gacggcgcgc attttccagg ctctgtgcat gcccatgctg attacatcgt tggcccggtc
     1261 gtagccgccg gcgatgcgct cgaactggtc gtgcacgtaa gctgcttttt cttctatggt
     1321 gggaagggac atcaggacac ccggtgttat ttaacgaagc cgaaccctac cacaaggcaa
     1381 tccagcatga ataaaccgaa aactatccag gtcaggcggt caagacctgc ttcggcccct
     1441 cttttacctg aaaataccgt ggcggcggcg ccgattcctg ccattccatc gcccttcggg
     1501 gcatgcagaa gaatcagacc gatcagggag acggccagga cggcaccgat tactagaaga
     1561 gctatgtaag ctgcactcat gagtctttat cacttataaa ctggcaaatg cctttgtagg
     1621 acggtaaaaa ttatatccta tatggaagcc gggcttgccg aaggtaaaga cagctggttc
     1681 acttagccga aatagaggtg ggacgtgtcc gagaagaaca aatcaggtaa ccatcacaag
     1741 gtagtaatag tgggtggcgg caccgccggg atctccgtag cggcaaggct catgcgtgcc
     1801 atcggcgatg tagacattgc cctgatcgag ccctcggaaa agcactatta ccagccgctc
     1861 tggacccttg tgggcggagg tatctgcgac aagtccgagt cagtaaaaga tgaatattca
     1921 ctcattccct caggtgttaa atggatcaaa gataaggtct ccgatgtcaa gccggactcc
     1981 aatctggtta agctggcctc aggtggcgag gttcactacg actatttgat tctcgcgccc
     2041 ggtttgcaaa ttgactggca caaaattccc ggtttgaccg agactcttgg gaagaacggc
     2101 gtttgcagca attatagcta cgagctggtg gataagacct gggagtttat atccaagttc
     2161 aagggcggca atgctgtctt tacccagccc aacacgcctg tgaagtgtgg cggcgcccct
     2221 cagaagattg cttatctggc agaggaagct ttcgtcaata gcggcgtgag agagcgcacc
     2281 aaggtcatat tttgttccgc caatgcgggc atcttctcag tcaaaaagta cgccaacaca
     2341 ctctccaagg tgttggagcg caagcagatt gaaaccaggt tccgtacaga cctgatcgcc
     2401 attaatggag ccgagcacaa ggctactttt aagcatctgg ataccggcgc cgaaagcacc
     2461 attgagttcg acatgatcca cgttacgccg cccatgagcg ctcccgactt tatcaaaggc
     2521 tctgctgttg ccgatcagac cggctgggtg gatgttgaca agaacaccct tcagcataaa
     2581 cgctacgaga atgtttttgc tcttggt
//
LOCUS       DAOQNZ010000495         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22565_length_2606_cov_3.698942, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000495 DAOQNZ010000000
VERSION     DAOQNZ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22565_length_2606_cov_3.698942"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PKW73_09620"
     CDS             <1..801
                     /locus_tag="PKW73_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73587.1"
                     /translation="MYANEAAHLPPLGQTMQQVKEREIERPGFAGAFVGPLLMDSLVD
                     GEISGSGIKLTDWILEIVARRKSDEPYVPFYNGIDFHAHEVLSANPDAVGKLISLGAE
                     SLAAMTATEEGFPIVGMEEHLARLAGSSDDFVCRTCSWHLAYHYRVLNSEGSRRGFVK
                     LVERDDVDVFLVFNPEELKDRPYAATIYPKQGALPDEKAWRWIDRLIPPQVRPPMEAN
                     DWPYQTPQISADSALFVWGAYMVKFHGDCSAKEWQRVWVKWPLPSDEW"
     gene            858..1562
                     /locus_tag="PKW73_09625"
     CDS             858..1562
                     /locus_tag="PKW73_09625"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNA73588.1"
                     /translation="MQEVLDKYRQFERDGFALLQAAVSPAQIEALRDAVETFRRENSV
                     CGAANMRNLLSGCPYVKSFAVAGLAHKIASEILGVSALPVRAILFDKTPAANWYVTWH
                     QDLSIPVKERLEIDGYGPWSTKEGVLHVQPPASVLEGMVALRIHLDPCPESNGAVKFV
                     AGSHLGGVLAPERVSGLREGGRAVVCPAEVGDVIAMRPLVLHASSVSQSPAHRRVLHI
                     EYAGVQLPAGLAWAEA"
     gene            complement(1584..2042)
                     /locus_tag="PKW73_09630"
     CDS             complement(1584..2042)
                     /locus_tag="PKW73_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73589.1"
                     /translation="MLPIFSTLVVVSGCSTKAGDDLAVTKLSESQIQATSFLPLNADK
                     PGTPVDVKDFLVAGKYTVVVYFSPYDGAYGNLEPGLMQLAQVRQDIAVRTINVNRPGV
                     QGIDLQGPIMINAQGQVLPYFQIYEPSRRLRAQARPAYEQVLQWLRDLPR"
     gene            complement(2188..>2606)
                     /locus_tag="PKW73_09635"
     CDS             complement(2188..>2606)
                     /locus_tag="PKW73_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73590.1"
                     /translation="SFYLNEGKPTFTYNWLGQAEYTIAASEALTPGANTIKFDFAYDG
                     GGPGKGGTGTISVNGKEVATGRIDKTQGFIFSADEGADVGADLGTAVSKAYQVPNRFT
                     GKLNSVKVDLLPGSAVDQAHNARAHSEGKMKKMLSD"
BASE COUNT          540 a          639 c          753 g          674 t
ORIGIN      
        1 atgtacgcaa acgaggcggc gcacctgccg ccgctggggc agaccatgca gcaggtaaaa
       61 gagcgggaaa tcgaacgtcc tggttttgcc ggtgcgtttg tcggtccgct actaatggac
      121 agccttgtgg atggcgaaat ctccggaagt ggcatcaagc tcaccgactg gattctagag
      181 attgtagcca ggcgaaagtc cgacgagccg tatgtgccct tttacaacgg cattgatttt
      241 catgcgcatg aagttctcag cgcaaacccc gatgcagtcg gcaaactaat ctcactgggg
      301 gctgagtctc tggctgctat gactgccact gaggaaggct ttccgattgt cggcatggaa
      361 gagcatctgg caaggctggc cggctccagc gatgattttg tctgtcgtac ttgttcatgg
      421 cacctggctt atcactatcg agttctaaat tccgaaggtt cgcgccgggg gttcgtaaag
      481 cttgtggaac gtgatgacgt ggacgttttt ctggttttta atccggaaga acttaaagat
      541 agaccctatg ctgccaccat ctatccaaag caaggcgcat tgcctgatga gaaagcctgg
      601 cggtggatcg atagattgat accgccgcag gtgcggccgc ccatggaggc taatgactgg
      661 ccatatcaaa ctccgcagat tagcgctgat tcagcccttt tcgtttgggg cgcatatatg
      721 gtcaaattcc acggagattg ttctgcgaaa gaatggcagc gggtctgggt caagtggcct
      781 ctccccagcg atgagtggta ggtcggggtt tgtcattacc gggagggttt tccaggcagt
      841 gctaaaatac ttgccatatg caggaagttc tcgataagta ccggcaattt gagagagatg
      901 gctttgcttt gttgcaggct gcggtgtcgc ctgcacagat agaagcattg cgggatgcgg
      961 tggagacctt tcgccgcgaa aactctgttt gtggtgccgc gaacatgcgc aatttgctga
     1021 gcggatgccc ttatgtgaaa tcgtttgcgg tggcgggtct cgctcataag attgcctctg
     1081 aaatattagg agtgtcggcc ttgccggtca gggctattct ctttgataaa acccctgccg
     1141 caaactggta tgtcacctgg catcaagact tgagcattcc cgtaaaagaa cggcttgaga
     1201 tcgacggtta tggaccctgg tctaccaagg aaggggtctt gcatgtgcag ccgcctgcct
     1261 ctgtgcttga gggaatggtg gcactgcgaa tacacctgga tccttgcccg gaatcaaatg
     1321 gtgccgtcaa gtttgttgcc ggttcccact tgggtggtgt attggcgccc gaaagggttt
     1381 ccggtctgcg tgagggcggc agggcagtgg tatgccctgc ggaagtagga gacgttatcg
     1441 ccatgcgacc acttgtactg cacgcctctt cggtttctca gtcgccggcc catcgaaggg
     1501 ttttgcacat tgagtatgcc ggggtccagc ttcctgcggg gcttgcctgg gcagaagctt
     1561 agggcgcaaa ccatgcttct tggttagcgg ggtagatctc tcagccactg taatacctgt
     1621 tcgtaagcag gtctggcctg ggctctcagt cggcgcgaag gctcatagat ttgaaagtaa
     1681 ggaagaacct gcccctgggc gtttatcatt attggccctt gcaggtcgat gccctgcaca
     1741 cccggtctgt ttacgttgat ggtgcgtacg gctatgtctt gtctgacttg tgccagctgc
     1801 attaagccag gttccaggtt gccataggca ccgtcgtaag ggctgaagta gaccacaact
     1861 gtgtacttgc ctgctaccag gaagtctttt acatctaccg gcgtgcccgg cttgtcggcg
     1921 ttcaggggca aaaagcttgt cgcttgtatc tgactttctg acagtttagt gacggccaga
     1981 tcgtcaccgg cttttgtgga acagccggaa acaacaacca gagttgagaa aatcggcaac
     2041 aagaaagtcg ctgattgctt tagcttgcgt tcgctaagtt tgcatggaag gttcataata
     2101 ttggcaattg tacttcctct gccaccgggt ttaccgttcc cgggcggcag aggaaaataa
     2161 aataaacctg gaagtaagct gcctagttta gtcggagagc atcttcttca ttttgccttc
     2221 gctatgagct ctggcattgt gagcttgatc gacggcgctt ccaggcagta agtctacttt
     2281 cacgctgttg agtttaccgg tgaagcgatt gggaacctga taagccttgc tcaccgctgt
     2341 tccaagatcg gcgcccacgt ctgcaccttc atcggctgag aagataaagc cctgcgtttt
     2401 gtcgatgcgt ccggtggcta cctctttgcc gtttacagag attgtgcctg taccgccttt
     2461 gcccggtccg ccgccatcat aggcaaagtc gaatttgatg gtgttggctc cgggggtcag
     2521 ggcttcactt gcggcgattg tgtattccgc ttgtcccagc caattgtagg tgaaggttgg
     2581 tttaccctca ttcaggtaaa agctcc
//
LOCUS       DAOQNZ010000496         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22615_length_2603_cov_3.658163, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000496 DAOQNZ010000000
VERSION     DAOQNZ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22615_length_2603_cov_3.658163"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PKW73_09640"
     CDS             <1..301
                     /locus_tag="PKW73_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73591.1"
                     /translation="TRRLIEGLRRNGVKTKLSEAFSYMQEHVEEEVLRDRAVVQTPVL
                     RKLWSGDDVVLSAMPVSPRAGLSEEPVSLNKLPSVKGSNTTPAKATGAKRGAKAK"
     gene            complement(431..1510)
                     /locus_tag="PKW73_09645"
     CDS             complement(431..1510)
                     /locus_tag="PKW73_09645"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNA73592.1"
                     /translation="MRHFLKPALISTVLVTVLTAGPACADEVSDFTVNARVGNTGNLT
                     VEESILIDFGKNQRHGLLRKIPTYFASDNAELEVLAAESPPGTNCHKVVSQLNQSLQL
                     KLGDPRALISGKHTYRLKYIIKNPAKKSGDSYTYEWSPNGKNWHMPIKTMTVNITGAT
                     AGISKSLAVTRQDSLPTATISSTGSNYTLKAQDLQPAQVVSVKINLPAPANQVTAAVA
                     PAVSEEKPGFLQKLTRPDHNLQVFLWIVGIVGSVLLLTALVKSQSNCSCRCGPVCSCS
                     CGCRKGCPCQSTSFKEEPVRARRSGSGSGYNSYSNNDPYYYNDDYDSGWSSSSSSSSS
                     WSSSDSSSSSSDSGGSGDSGGGDSW"
     gene            1723..2427
                     /locus_tag="PKW73_09650"
     CDS             1723..2427
                     /locus_tag="PKW73_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNA73593.1"
                     /translation="MKRRHVEAHFTAGPLVRDVVIGMSDGLTVPFALAAGLSGAVTAT
                     GLIITAGLAEVAAGAIAMGLGGYLAARGEAEHYVQEKRREEQEVKEIPFQEVLEVRQI
                     FAAYGIDEKALKPIEEAFQANPDKWVDFMMRFELDLAEPEPGRARQSAFTIAFAYALG
                     GMIPLAPYFFIARVGDALIMSVVFTLLALLAFGFVKGKFVGLNPWRSALQALVVGGLA
                     AGVAFGVAKLVNGAGA"
BASE COUNT          536 a          595 c          780 g          692 t
ORIGIN      
        1 cacgcgccgc ctcattgaag gattgcgccg caatggcgtc aagacaaagt tgtcggaagc
       61 cttttcttac atgcaagaac acgtggaaga ggaagtgttg cgtgacagag ctgtggtgca
      121 gacgccggtg ttgaggaagc tctggtcggg ggatgacgtt gtactttcgg ctatgccggt
      181 cagtcctcgt gccggtttgt cggaagaacc cgttagtctg aacaaattgc catccgtaaa
      241 aggaagtaac accacaccag cgaaagctac aggcgctaag cggggcgcca aagctaagta
      301 gccaggggca aagatggaac atccaatatg cggctgtctg caaatgacta gcttggttgg
      361 ttggttgttt ggttggttgg ttgtttggtt ggttgcgcca atagactttc ctgctttagc
      421 aagacagcaa ttaccacgaa tctccgccgc cgctatcgcc ggagccgcct gagtcggaac
      481 ttgaactact ggaatcggaa ctgctccagg aactactgct ggagcttgag ctactccaac
      541 cactgtcgta gtcatcgttg taataatagg gatcgttgtt gctgtagctg ttgtagccgc
      601 tgccggagcc tgatcttcgg gctctgaccg gttcttcctt gaagcttgta ctctggcagg
      661 ggcagccctt gcggcaaccg caagagcaac tgcagaccgg accacagcgg caactgcaat
      721 tggactgcga tttaaccagg gccgtaagca agagtaccga gcccactatg cccacaatcc
      781 atagaaacac ttgcaagttg tgatcgggtc tggtgagctt ttgcagaaaa cccggttttt
      841 cttcgctaac cgcaggtgcg acggcggctg tcacttgatt cgctggcgca ggcaggttta
      901 ttttgactga tactacctgg gccggctgta ggtcttgagc cttgagggtg taattgctgc
      961 cggtactgct gatagtggca gtaggcaggc tgtcttgcct ggttaccgcc agtgacttgc
     1021 ttatgccggc agtggctcca gtgatattga cggtcatggt cttaatcggc atatgccagt
     1081 tcttgccgtt gggagaccat tcgtatgtgt aagagtcgcc gcttttcttg gccgggtttt
     1141 tgatgatgta tttaagtcta tatgtgtgtt tgccggagat aagcgctctg ggatcgccta
     1201 gtttgagttg gagagactgg ttcaattggg agacgacttt gtggcagttt gtgccgggtg
     1261 gagattcagc agccagtacc tctagctcgg cattgtccga ggcaaagtag gtgggtattt
     1321 ttcgcagcag tccgtgtctc tggttcttac cgaagtcaat gagaatgctc tcttccaccg
     1381 tcaggttgcc tgtgtttcct accctggcgt tgaccgtgaa gtcgctcact tcgtcggcgc
     1441 aggcgggacc tgcggttaaa actgtgacaa gtacggtaga gatgagtgca ggttttagaa
     1501 aatggcgcat cgagtatctc ccttaagcga tctcttggca actctagtat tgattcggaa
     1561 cggttaagga gcggttatag gagcatgcat tctgcttcgg tttaagaatg atataagatt
     1621 ttctgagaat ttgtttattc tgctggtagc tcctggagag gaggacaaag tcttgccttg
     1681 cccttacgct aagtgccgga ctcaacaaaa ggctggtata tcttgaagag acgacatgta
     1741 gaagctcatt ttacggcggg gcccctggtt cgcgacgtcg ttatcggtat gtcggacggt
     1801 ttgaccgttc cctttgccct tgccgctggt ctgtccggtg ctgttactgc tactggtttg
     1861 attattactg ccggactggc ggaagtggcg gcgggagcca tcgccatggg cttgggcggc
     1921 tatctggcgg ctcgcgggga agcagagcac tacgtgcagg agaagcggcg agaagagcag
     1981 gaagtaaagg aaattccctt tcaagaagtc ctggaagtgc ggcagatatt cgctgcttat
     2041 ggaatcgacg agaaggctct caaacccatt gaggaggcct ttcaggccaa tcccgataag
     2101 tgggtggatt tcatgatgcg cttcgaactc gatcttgccg aaccggaacc tggacgtgcc
     2161 aggcagagtg cctttaccat tgcctttgct tatgccctgg gaggcatgat cccgctggcg
     2221 ccttatttct ttattgccag agtgggcgat gctctcatca tgtcggttgt ctttacccta
     2281 ttggctctct tagcttttgg ttttgtcaaa ggtaagtttg tcggtctcaa cccctggcgc
     2341 tcggcactgc aggccctggt tgtgggtggt ctggctgccg gtgtggcttt tggggtggca
     2401 aaactggtga atggggcggg ggcttagcat tttaggaatg cctttatgaa ccggtctcaa
     2461 ggtttgattc cggctgacgc aggtaaactt tagtggactc attggtggag gtgttgatgc
     2521 aggaagcaga tatcagaccg gcggagatgt tcaaggagag cctcaggctt gatcaggtag
     2581 atattgctcg ggtacttgct cac
//
LOCUS       DAOQNZ010000497         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22646_length_2601_cov_4.261980, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000497 DAOQNZ010000000
VERSION     DAOQNZ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22646_length_2601_cov_4.261980"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PKW73_09655"
     CDS             complement(<1..558)
                     /locus_tag="PKW73_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNA73594.1"
                     /translation="MILVDKHTKVVVQGATGKMGAAHTKRMLDYGTNIVAGVTPGKGG
                     EKIHNVPVFDTVMEAVKTTGATCSVIFVPPYLALDAGYEALDAGISLLVLITEGIPPQ
                     DTAKLAAAVKAKGAKLIGPNCPGIITPGQSLIGILPGSIFKSGPVGMVSKSGTLTYEI
                     ALALTESGYGQSTCVGTGGDPIKGLD"
     gene            complement(571..1713)
                     /locus_tag="PKW73_09660"
     CDS             complement(571..1713)
                     /locus_tag="PKW73_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNA73595.1"
                     /translation="MNLYEYEAKRIFEAGGIPVPPAVRITKPEELAKVHFGYPLTLKC
                     QVMSGGRGKAGGIKFAKDLEEGKALAKDLLQLVIKGSKTESLLVEPMVSIAKELYLAV
                     TLDRERGCPIFIASAEGGIEIESSHNVLTEPIPFPYHPYVGRNLAAKLGLKGAVMAKV
                     ADLANKLYKVFESKDLDLAEINPLVITKGDDVLALDGKIIINDDSIGRQPAFKGWMEA
                     HLVDMSPRERRAKLTDLNLVELDGNIGILCNGAGLTMATMDMVKAFGGQPGNFLDAGG
                     GSDQKKTLAALELVNENPAVDVILLNILGGITACDEVAGALVEFMKKYPERQMVVRLR
                     GNNQDVAEKMLAASGLKLYPDLEEAVKEAVSRSKNATKKPALAAKA"
     gene            2061..>2601
                     /locus_tag="PKW73_09665"
     CDS             2061..>2601
                     /locus_tag="PKW73_09665"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNA73596.1"
                     /translation="MRIGILTGGGDCPGLNAVLRAVVRRSVKEYGSEVVGYLEGFRGV
                     MEDMIMPLTMHSTGGLIHRGGTILRTSRTNPFKVERGPERIKENMQKHRLDALIVVGG
                     EDTCGVANRLYKEHNLNLVCVPKTIDNDLNATDVTFGFDTAVNIVAEALDRLHTTAES
                     HNRVLVCEVMGRNAGWIACY"
BASE COUNT          527 a          672 c          734 g          668 t
ORIGIN      
        1 gtcgagacct ttgatggggt ctccaccggt gccgacgcag gtagactgac cgtaaccgct
       61 ctcagtgaga gcgagtgcga tttcataggt gagggtgccg gacttgctga ccatgcccac
      121 aggacctgat ttgaaaatgg aaccaggcaa gattccgatc agggactgac cgggggtgat
      181 gattcccggg cagttgggtc cgatcaattt agcgcctttg gctttcactg ccgctgccaa
      241 cttggctgta tcttgcggcg ggatgccttc ggtgatgagc accagcaggc tgattccggc
      301 atcaagtgct tcataaccgg catcgagggc caggtagggc ggcacgaata ttaccgaaca
      361 ggtggcgccg gtggtcttca ccgcttccat gacggtatcg aagaccggta cgttgtggat
      421 tttttctcca cctttaccgg gggtgacgcc ggcaacaata ttggtgccgt agtcaagcat
      481 gcgcttggtg tgagcggcac ccattttgcc ggtggcgccc tgcacgacta cttttgtgtg
      541 tttatcgaca agaatcactt caattctcct ttaggctttg gcggcaagag ccggtttttt
      601 tgtggcgttc tttgagcggc tgaccgcctc tttcactgct tcttccagat cgggatagag
      661 cttcagtccg gatgcggcca gcattttttc cgctacatcc tggttgttgc cgcgcaatct
      721 cactaccatc tggcgctcgg ggtatttttt cataaactcc accagtgctc cggctacttc
      781 atcacaggct gtgattccgc ccaggatatt gaggagaatg acgtccacgg cagggttctc
      841 attcacgagt tcgagggctg ccagggtctt cttctggtcg gagccgccgc cggcatcgag
      901 gaagttaccg ggttggccgc cgaaagcctt gaccatatcc atggttgcca tggtcagacc
      961 ggcgccgttg cagaggatac cgatattgcc gtccaactcc accaggttga gatcggtcaa
     1021 tttggcgcgg cgctcccggg ggctcatgtc taccaggtga gcttccatcc agcctttgaa
     1081 agccggttgg cgtccgatgg aatcatcgtt gatgatgatt ttgccatcga gagccagcac
     1141 atcgtcgccc ttggtgatga ccagagggtt aatctcagcc agatcgaggt ctttgctttc
     1201 gaatacttta tagagtttgt tggccaggtc tgccacttta gccatgaccg cacccttcaa
     1261 accaagtttg gcagccaggt tgcggccgac ataaggatga taggggaagg gaatcggctc
     1321 ggtaagtacg ttgtgggagg attcaatctc aatgccgcct tcagcggagg caatgaagat
     1381 cgggcagcct ctttctctgt ccagggtgac ggccagataa agctctttgg cgatggacac
     1441 catgggctct accagaaggc tctcggtttt ggagcccttg atgaccaact gaagcaggtc
     1501 tttggccagg gccttacctt cttccaggtc tttggcgaat ttaatgccgc cggccttacc
     1561 tcttccacct gacatgacct ggcatttcaa ggtgagtgga tagccgaaat gtactttagc
     1621 taactcttcc ggtttggtga tacgtactgc cggtggtacg ggtatgccgc cagcttcaaa
     1681 gattcgcttg gcttcgtatt cgtagagatt caatttattt cgaccctcac tggttacaag
     1741 gtggcctcaa ggcttataca gacccttaat gtacgacaga aagctcggcc gggctttgac
     1801 ctttaggttt ttatccattc cgccggggga ttttccaagg ggtacaggaa tttcaaccga
     1861 tttggcttga ggttctcaat attccgctta ataatccgac tacaaaggga ggcatgaaca
     1921 gggcgctttc agggtctgca attatactta gggcgttcgg caagtatccg gacctctagt
     1981 atagtccccg gctcttggct tctcgggctg agccaatctt gctcctgcgt ctgacactta
     2041 atggtcttga ggttttttag atgcgcatcg gtatccttac tggcggcggc gattgtcccg
     2101 gtctcaacgc cgtattgagg gcagtggtca ggcgctccgt gaaagagtac ggttcggaag
     2161 tagtgggtta cctcgagggc ttccggggcg tcatggaaga catgattatg cccctgacca
     2221 tgcattccac cggtggtctg attcatagag gcggtaccat cttgcgtacc tcccgtacca
     2281 atcccttcaa agtggaaaga ggaccggagc gaatcaaaga gaatatgcag aagcatcgcc
     2341 tcgacgcctt gattgtagtg ggcggcgagg atacctgcgg cgtggccaat cgcctctata
     2401 aggagcacaa tctcaatctg gtctgcgtgc ctaagaccat agacaatgac ttgaatgcca
     2461 ccgatgtcac cttcggcttt gatacggccg tcaatattgt ggctgaggct ctggaccggt
     2521 tgcacaccac ggccgagtct cataaccgcg ttctcgtttg tgaagtgatg ggacgcaatg
     2581 ccggctggat agcttgttat g
//
LOCUS       DAOQNZ010000498         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22666_length_2600_cov_5.075049, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000498 DAOQNZ010000000
VERSION     DAOQNZ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22666_length_2600_cov_5.075049"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1474
                     /locus_tag="PKW73_09670"
     CDS             212..1474
                     /locus_tag="PKW73_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73597.1"
                     /translation="MNEHSRSHDKASKSSSSRTGGETQVKSGGYNLGSVLLQMSTIIS
                     PEQLGEAQQMARQTGMDVLKMLTMNGYINEKTARLAKDAAFVVRQDVFDEEQVAQALY
                     RAVKEGIELEQALAVMSSAGQVQAVRAKTSYTPLSELLILAGILQSGELKSYQATANE
                     TGLPLGRVITHAGVVNPESVMAALCAQVYEREGYFHKDELVLILTTCYRKSMSFQDAL
                     EFHNKAIPEEALQVRLGELLTQCGLVPRNAVISALELSLLRGTPLGQELVSMGQIKEE
                     ALEKALLIKELVRKGRLADEHVPAIVRRVVINGQDSDVAMGEVAAMVLDDSDFDSLDR
                     VLCYSGITQEALIVPIRLSLSENAPFMEYLAALVDKQIIDEKCALAAARVLYLVRLDA
                     LSGDQAVSLLKYCREYQTSVDQAMDKLH"
     gene            complement(1486..2388)
                     /locus_tag="PKW73_09675"
     CDS             complement(1486..2388)
                     /locus_tag="PKW73_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNA73598.1"
                     /translation="MKDIERAVAYRSVLEVCNSTAGKEGAGFSILRPFPTRTLSYVDP
                     FLLLDHMQPVPLKPGEAKGAPDHPHRGFETVTYLLEGAMEHRDSAGNFGRLTAGDVQW
                     MTAGAGVVHSEMPGPELLQHGGVLHGFQIWVDLPAALKMTAPRYQDLSSAKIPVAVSA
                     DGLTEVKVIAGESLGIKAPTGTHTPITFLHIKMAAGAVFTQPLGSGQNALVYIVSGTA
                     RFGPGAETVEGTGKMVRFALEDRSLYLENAGADDRLDLLVLAGQPLNQPVARYGPFVM
                     NSEAEIEQALEDYRSGRMGQIAGA"
BASE COUNT          633 a          743 c          690 g          534 t
ORIGIN      
        1 aatactgctg cccgaggcta taaagcctga ttatcccaga taaacacgaa cactctccct
       61 tgacaaggtt aagtagcgtg aacattcatt gcagaactta tctgacaaaa tagttcaaac
      121 gtggaacata accatagaga cgctaccccc ctgtcagcac agcatccact cagaagctaa
      181 tgcattcgag ccttttcagt caggccaatt gatgaacgag cattcgcgct cccatgacaa
      241 agcctccaag tcgtcctcca gcaggacggg cggtgagaca caggtaaaat cggggggtta
      301 caatctgggc tcggtgctct tgcaaatgtc cacaattatc agccccgaac agcttggcga
      361 agcgcaacaa atggccaggc aaacaggcat ggacgtgctg aaaatgctga ccatgaacgg
      421 ctacatcaac gagaaaacgg caagactggc aaaagacgcc gcttttgttg tcaggcagga
      481 cgtctttgac gaagagcagg tagcccaggc cctgtaccgg gccgtcaaag aaggcatcga
      541 actggagcag gctctggcag tcatgagttc agccggacag gtccaggccg tgagagccaa
      601 gaccagctac acacctctgt cggaactgct tatcctggcc ggaattttgc aaagcggtga
      661 gctcaaatct tatcaggcca ccgctaacga aaccggtctg cccctcggtc gggtcatcac
      721 ccatgccggt gtggtcaatc ccgagtcggt gatggccgct ctttgcgccc aggtttacga
      781 aagggaaggc tattttcaca aggacgagct ggttttgatt ttgaccactt gctaccgcaa
      841 gagcatgagt tttcaggatg ccctcgagtt ccacaacaaa gcaataccgg aagaagccct
      901 gcaggtaaga ctgggagaac ttttgactca gtgcggtctg gtaccgcgca atgctgttat
      961 ttcagccctg gaattgagcc tgctcagggg cacccccctg ggacaggagc ttgtctccat
     1021 gggacaaatc aaggaagagg ctcttgaaaa agcgcttcta atcaaagagc ttgtcagaaa
     1081 gggccgcctg gcggatgagc atgtgccggc tatagtgcgg cgagttgtaa taaacggcca
     1141 ggattcagat gtagccatgg gcgaagtggc agccatggta ctggatgaca gcgacttcga
     1201 cagcctggac cgcgtgctct gctattcagg cattacccag gaggcgctca tcgtacccat
     1261 ccgcttgtcg ctctccgaga acgcgccctt catggaatac ctcgccgccc tggtggacaa
     1321 acaaatcata gacgaaaagt gcgccctggc agcagcacgg gtgctctacc tggtgcgcct
     1381 ggacgccttg agcggcgacc aggcagtttc ccttctaaaa tattgccggg aatatcagac
     1441 ttccgtagac caggccatgg acaagctcca ttgagcaaag ggaaattaag cgccggcgat
     1501 ctgccccatc cttccacttc ggtaatcctc aagggcctgc tcaatttccg cttcgctgtt
     1561 catgacgaaa ggaccgtaac gagccaccgg ctggttgaga ggctggccgg ccagcaccag
     1621 caaatcgagc ctgtcatccg ccccggcgtt ttccagatac aaactgcggt cctcaagggc
     1681 aaagcgaacc attttgcccg tcccttccac ggtctcagcc cccggaccaa agcgagccgt
     1741 gcctgagact atatagacga gggcattctg tccgctgccg agcggttgag taaaaacagc
     1801 cccggcagcc attttgatat gcaagaaagt aatcggcgta tgggtcccgg tgggagcctt
     1861 aatccccaaa ctttcaccgg cgatgacttt cacttccgtc aaaccatcgg cactgactgc
     1921 cacaggtatc ttagccgaag agaggtcctg atagcggggg gcggtcattt tgagagcggc
     1981 cggcaggtcc acccaaattt gaaagccgtg caagacgccg ccatgctgca agagttcagg
     2041 gcccggcatc tccgagtgca caacaccggc accggccgtc atccactgca cgtcaccggc
     2101 ggtcaatctt ccgaaattcc cggccgaatc gcggtgctcc atggctcctt ccagtaaata
     2161 ggtaaccgtt tcaaagccac gatgaggatg gtccggggcg cctttggctt caccgggctt
     2221 gaggggcacc ggctgcatat gatcaagaag caggaaggga tccacataac tcaaggttct
     2281 cgtagggaaa ggtctcagta tggaaaatcc agctccttcc ttccccgccg tggaattgca
     2341 gacttccagc acagaccgat aggcaaccgc cctctcaata tctttcatag tctattacct
     2401 ctttggtttc cgtaaaacaa gttcagggca ggacatccag agcatcggct cctttgccga
     2461 ccttcttcag cagggtgagg agcgtatctt gctcctcctc cgttaaagca gagacagccc
     2521 ggcgcagatg cctctggtgc tgcttgaatg cctcctttat cagtttttta ccggagcggg
     2581 tgaggcgcac caaccgcacc
//
LOCUS       DAOQNZ010000499         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22671_length_2600_cov_4.101768, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000499 DAOQNZ010000000
VERSION     DAOQNZ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22671_length_2600_cov_4.101768"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKW73_09680"
     CDS             complement(<1..514)
                     /locus_tag="PKW73_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA73599.1"
                     /translation="MSPRKPNEQPVLELKNIHKSFGDRQILKGVSFNVYAGEMLGLIG
                     PSGCGKSTILKIICGLLEPDEGEVIKRSDEIGLVFQGSALLNSLTVRENLAIALQRRK
                     LGKGEENRIISERLKMVGLEGYEDYSPTKLSGGQQKRTSFARAIVNDPKIILYDEPTT
                     GLDPVMSTIIE"
     gene            complement(523..1332)
                     /locus_tag="PKW73_09685"
     CDS             complement(523..1332)
                     /locus_tag="PKW73_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA73600.1"
                     /translation="MTTSAPRDKRLPNPYRMVEDQFAIGVQLLGQVLILWFQTLSYMV
                     RGQIDLKETVRQAYMIGVLSFVVVGITALFTGFAMSLQISRELVQFGADTAIGGVVSL
                     ALVREIGPITAGVIVAGRVGSSVAAELTTMMITEQIDALKVMRVDPVKFLVVPRYTAA
                     LVMIPTLSVYAMFIGLIAGIVIAQGAANVPPDTFMDSVKQTILDRDFSVHFLKSIINA
                     TLIIMFSCAIGLHTRGGAEDVGIATTRTIVWTMISIFILNFVVTSLTYAPR"
     gene            1542..2360
                     /locus_tag="PKW73_09690"
     CDS             1542..2360
                     /locus_tag="PKW73_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA73601.1"
                     /translation="MEEGGHEPGKDSREKKNKGAILGSLGVLGIAAFKFKSFLLVGLS
                     SFKFAVFFKSFFSMFLMLGLYTVTFGWTYAFIVVGILLIHEMGHYIWMKRHGLEPHAP
                     IFVPFLGAAVAMGKMPDNQAVNAWVAMAGPMLGGYTSILFYYIGAQYGLTYLMAAGWT
                     GVMLNLFQLIPVRPFDGGFITGAISKWFMLPGALMIFTLAMLWQTPLMFIIALLAGFS
                     VFRAFTGGGLGDLGVKPATAGQKFAIGATYLVLALSLGYIYSIADANLVSVLRK"
BASE COUNT          570 a          644 c          716 g          670 t
ORIGIN      
        1 cttcaataat tgtcgacata accggatcaa gaccggtggt cggttcgtcg taaagaatga
       61 tcttgggatc attgacgatg gcccgggcaa agctggtgcg cttttgttgt ccaccggaaa
      121 gcttggtggg tgagtaatcc tcatagcctt ccagcccaac cattttcagt ctttcggaga
      181 taatgcgatt ctcctcgcct ttgcccagtt tgcgtctttg caaggcgatg gccaggtttt
      241 ctcgcacggt aagagagttc aacagggcgg aaccctgaaa gaccagaccg atttcgtctg
      301 aacgcttgat cacttcgcct tcatcgggtt ccagcaaacc gcagatgatt ttcaaaatcg
      361 tagacttgcc gcaaccggag ggaccaatca agccgagcat ttcgccggca tagacgttga
      421 agctcacccc tttgagaatc tggcgatcgc caaaactctt gtggatgttt ttcaactcca
      481 gcaccggctg ttcattcggc tttcgagggc tcatggcggc tcctagcgcg gtgcgtaagt
      541 gagggaagta acgacgaaat tgagaatgaa gatggagatc atcgtccaga cgatagtacg
      601 ggttgtggcg atgcccacat cttcagcgcc gcccctggtg tgcagcccga tggcgcaact
      661 gaacataatg atgagggtgg cattaataat ggatttgagg aagtggaccg agaagtcgcg
      721 gtccagaatg gtttgtttga cggagtccat gaaggtgtcg ggcggcacat tggcggcgcc
      781 ctgggcaatg acgataccgg cgatcagacc gatgaacatg gcgtaaacgg aaagggtcgg
      841 gatcatgaca agtgcggcgg tgtagcgcgg caccaccagg aatttgacag ggtctacgcg
      901 catcaccttg agggcgtcga tttgctcggt gatcatcatg gtggtaagtt ctgccgccac
      961 tgatgagcct acgcgaccgg ccactattac gccggctgtg atcggtccga tttctctcac
     1021 cagggctagt gaaacaacgc cgccgatggc agtgtcggcc ccaaactgca caagttctct
     1081 tgatatctgc agggacatgg caaagccggt gaatagtgcc gtaatgccca ccaccacaaa
     1141 ggaaaggacg ccgatcatat aagcctggcg cacagtctct ttcagatcaa tttgcccgcg
     1201 caccatgtaa gaaagggtct gaaaccacag tatgagtacc tgtcccagga gttgcacacc
     1261 gatggcaaat tggtcctcta ccatgcggta ggggttggga aggcgcttat ctcttggggc
     1321 actggttgtc atctttgtaa ttctatttgc taatggctcc taaattagct ctttgcacaa
     1381 cacttaatgt ggtgtgtggc gtttattgag gtaaacggct tgccacaaat atgtgatgac
     1441 tgcagcctgg cctaatagtt tccggtattt ggcgagtttg acgatatatc aatgagatcg
     1501 gttatatgat gtgaagagtc cagctaaaga ggtgtgtact gatggaagag ggcggccacg
     1561 agcctggcaa agattccaga gaaaagaaga acaaaggcgc cattttaggc agtctcggag
     1621 tgcttggcat cgcggccttc aagttcaaga gttttcttct ggttggtctg agctctttca
     1681 agtttgccgt tttcttcaag agcttctttt cgatgttctt gatgcttggt ctttacacgg
     1741 tcacctttgg ttggacctat gccttcattg tggtgggaat tctgctgatt cacgaaatgg
     1801 ggcattatat ctggatgaaa cgccacggac tggagcccca tgccccaata tttgtgccgt
     1861 tcttaggcgc ggccgtagcc atgggaaaga tgccggacaa tcaggccgta aacgcctggg
     1921 tagccatggc cggtcccatg ctggggggct atacctccat tcttttctac tacataggcg
     1981 ctcagtacgg gctgacttat ttgatggctg ccggttggac cggtgtcatg ctcaatttgt
     2041 tccagcttat tccggtgcga ccctttgacg gcggcttcat aaccggggcc atcagcaaat
     2101 ggttcatgtt gccaggcgcc ctcatgattt tcaccctggc catgctctgg cagacgccat
     2161 tgatgtttat cattgccctc ctggcgggtt tttccgtctt ccgggctttc accggaggcg
     2221 gtcttggcga tcttggtgtg aaaccggcaa cggccggcca gaaattcgcc atcggcgcca
     2281 cttacctggt gcttgctctc agtctcggtt atatctattc cattgccgat gccaacctgg
     2341 tcagcgtctt gagaaagtag ttgagaaggt ggtaattcca ccatagccaa agggcagtcg
     2401 tttcgcttca atagaagcct gacgttgatt ttggcttgtt gttggatttt tcctttcaca
     2461 tgcactggaa caggtaaccc atttgaaagc ccagaacccc tatgaaggtt ttttcgagcg
     2521 ttccctggtt gatgccgggc cggaccgcat tgctgcctct cccgaacaga gtttagccag
     2581 ggaattgccc agccttgcct
//
LOCUS       DAOQNZ010000500         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22676_length_2600_cov_3.363458, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000500 DAOQNZ010000000
VERSION     DAOQNZ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22676_length_2600_cov_3.363458"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /locus_tag="PKW73_09695"
     CDS             complement(<1..1405)
                     /locus_tag="PKW73_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA73602.1"
                     /translation="MLERKVVARIGVPLTFCFLFFGLQSLLIAKEKAPSQDQVIVADG
                     GASQPLPFIAQLAKSANSDPQSLYNQSWKLIKDQYYDQKFNGQNWSRWQHHYDTKLKT
                     MDDSHKAIETMLASLADPYTRFLDKEAFTEEKDQIEAHLFGVGMQLGMNFEHKVVVIA
                     PILDTPASQAGMMSGDEIVEVDGKSIKGQSLDQVVKQIRGPIATEVEIGFLRKGERKK
                     VKLKRAEIPIKAVTTAEYLPGNIGYIRLESFISNKATDEIKKALNQLKNADGIILDLR
                     HNPGGLLTNAIEMAGLFLPEKSIIVSTIDADGYKQSQAVVGQPSCTLPLVVLINKGSA
                     SASEILSGALRDNGRAKLVGEKSFGKGLVQAINRLSDGSGINVTIAKYLTPNDTDIHK
                     TGIVPDFQVKVDDDLATDTKDKERKEYKGPWWIDVTMKEFKKRSPNDGKDIQLLKAQD
                     VLREEIAKIHTANPNGPK"
     gene            1919..>2600
                     /locus_tag="PKW73_09700"
     CDS             1919..>2600
                     /locus_tag="PKW73_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNA73603.1"
                     /translation="MSPSPGAGLTTTLEKGGPDITDKVVGQSADHSAALSKEAESPVS
                     GTSISARLSSIKISKSTKLRVKILISLVLFASLFIFGKVDLRKAVEVGLKADKGYLLL
                     AVILSLASTFLNAHRWQLLAAAVGLEKGLLKLVQFCFVGLFFNLFLPSTVGGDFSRCY
                     YLSKGTGKYVNAFYSVLADRTVGIAVLFLFASVGILVGPGGGGLPIQLKLPIWLGTLA
                     IFSIVPFMP"
BASE COUNT          554 a          640 c          659 g          747 t
ORIGIN      
        1 ccttgggacc gtttggattg gcagtatgta ttttggctat ctcttcacgc agtacatctt
       61 gagctttcaa caattggata tctttgccgt cgttggggct gcgttttttg aattccttca
      121 tggtgacgtc gatccaccag ggacctttgt attccttgcg ctccttgtct ttggtatcgg
      181 ttgccagatc gtcatctacc ttgacttgga agtcgggtac gatgccggtt ttatgtatat
      241 cggtatcgtt gggtgtgagg tatttggcaa tggtgacgtt aataccggaa ccatcggaaa
      301 gtctgttgat ggcttgaacg agccccttac cgaaagactt ctcgccgacc agcttggctc
      361 tgccgttgtc gcgcagggct cctgaaagaa tttcactggc cgaagccgaa cctttattga
      421 tcagtaccac caggggcagt gtgcaactcg gctggcccac caccgcctgc gactgcttat
      481 agccgtcggc atcgatggtg ctgacgataa tgctcttctc ggggaggaag agaccggcca
      541 tttcaatggc attggtcagc agtccgccgg gattatgtct caggtcaagg ataataccgt
      601 cggcattttt caattgattg agagctttct tgatctcatc ggtggctttg ttggagatga
      661 aactctccaa cctgatgtat ccgatatttc caggcagata ttcagcagtg gtgacggcct
      721 tgattgggat ctcggctctt ttcagtttca ctttcttgcg ctcgcctttg cgcaagaaac
      781 cgatttccac ttcggtggca atcggtccgc ggatttgttt aaccacttga tcaagggact
      841 gacccttgat tgacttcccg tctacttcaa cgatttcgtc tccggacatc atgccggctt
      901 gtgaagcagg agtatcgagg atgggagcaa tcaccactac tttatgttcg aagttcatgc
      961 ccaattgcat gcccactccg aaaaggtgag cttcgatctg atctttttct tcagtgaaag
     1021 cttctttgtc gaggaagcga gtatagggat cggccagact ggccagcatg gtttcgatag
     1081 ccttatggga atcgtccatg gtctttaact ttgtgtcgta atgatgttgc cagcgcgacc
     1141 agttttgacc gttgaatttt tgatcgtagt actggtcttt gattagtttc caggactggt
     1201 tgtaaaggga ttgtggatcg ctattggcgc tcttggctaa ttgggcgatg aaaggcaggg
     1261 gctgggaggc gccgccgtcg gccacgatca cctgatcttg agaaggggcc ttctctttgg
     1321 cgataagaag gctttgtaag ccaaagaaga ggaagcagaa tgtaagcgga acgcctattc
     1381 gtgcaacaac ttttctctct aacaacttga gtacctctgc ctgctctatt tcttgattta
     1441 actacaactt gcgccactta tgccaatctt agatcactat gccctcgtgg tgccccaagt
     1501 agcgccgaag tcgcgccaat ttgtaataag tgacggtcat taaccaaaga attactaaga
     1561 ataatcgcca ctctttaagc tttcttgaga ttatttaatt gtagccggtt ttgccgcccg
     1621 ggcctgttca acttgcagcc tcggcggtcc accttgagaa gtacggtcta tatgttaaat
     1681 ttccagggtt ttaaatatct tggcatttgc cgtattattt tttaagctcc tggcaaagag
     1741 cctgaatccc tgttcagcgc gcaattccag gaattgatcc cctcccctga aggaataaaa
     1801 tctatacctt ctggagtcac ttccatctct gaaagatatg ctctgctagg gcgcttgact
     1861 ggggttcaga cataaattta gttgcaaggt taccgagaga agcccgagga attcagacat
     1921 gtcgccaagc cctggagcgg gactgactac taccctggaa aagggcggac ctgatataac
     1981 cgacaaggtc gtgggtcagt ccgccgacca cagtgctgcc ctttccaagg aagcggaatc
     2041 tccagtgtcc ggtacatcga tcagcgccag gctcagctct ataaaaattt ccaagagcac
     2101 caagctgcgc gtcaaaattc tcatttcgct ggtgcttttt gccagtctct tcattttcgg
     2161 caaagttgat ttgcgcaagg ctgtggaagt ggggctcaag gctgacaaag gttatttact
     2221 cctggctgta attctttctc tggcatccac ctttttaaat gcgcaccgct ggcagctgtt
     2281 ggctgctgcc gtcgggctgg aaaaaggttt actgaagctg gtgcagttct gcttcgtggg
     2341 gctcttcttc aacttgtttc tgccctcaac ggtaggaggc gacttcagcc gctgctatta
     2401 cctgtccaag ggcaccggca aatatgtgaa cgccttctat tccgtcctgg ccgaccgcac
     2461 agtgggaata gccgtacttt tcctgtttgc ctcggtgggt atcctggttg gaccgggagg
     2521 cggcggactg cccattcaac tgaaactacc catctggctc ggtaccctgg ccatcttttc
     2581 gatagttccg ttcatgccgc
//
LOCUS       DAOQNZ010000501         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22684_length_2599_cov_4.038915, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000501 DAOQNZ010000000
VERSION     DAOQNZ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22684_length_2599_cov_4.038915"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="PKW73_09705"
     CDS             complement(<1..1691)
                     /locus_tag="PKW73_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA73604.1"
                     /translation="MTEQSKHSHLGIIIQGSLSAGLEMRLDASQSVEDLRVGRFVVVE
                     GANTRFFSMVTDVTLSSSNPNILFNPPGTDPFLMAVLSGTSTFGTVKLQPMLMLEKQA
                     LAAFSLGSAATLSEEENEGRGLRPVKTIPSHFSQVFEASAEDFALVFGKEDNTPGNTY
                     FSIGRPLNMEVPVCVNLNRFIERSNGIFGKSGTGKSFLNRIFLSGIISKDLASVLVFD
                     MHNEYGWQAMSESKSAPRVKGLKQLFGHQVVVFSLDAESSKARGIPDARELYIGYNQI
                     EIEDLDLLKNELALSEATLENAYIAREKLGQDWIATLLDMSGEEIEEFTQVHKGHPVA
                     LKTLQRRLNTIVQKTPYLRPRVNHNYIEEILQQIQAGKHIVLEFGRQNNLLSYMLATN
                     IITRRIHAEYVRQSERYICDPERVNPPRKLVIVIEEAHKFLSPQVARQTIFGIIAREM
                     RKYFVTLLIVDQRPSGIDPEILSQIGTRVTALLNDDKDIDAVFTGVSGSQTLRTVLSQ
                     MDPKQQALVLGYAVPMPVVVRTRAFDSEFYKAVAPDGEAHDWRRLARGSREERQIL"
     gene            complement(1709..1978)
                     /locus_tag="PKW73_09710"
     CDS             complement(1709..1978)
                     /locus_tag="PKW73_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73605.1"
                     /translation="MLTQRAQWPHTVSDITKALDGVWGLCGAHGLNGNLYRLERSLHE
                     PIVYTFTEYRGEDESDIVKREEYSLEDRQIAVDQFAKALGFGQSN"
     gene            complement(2111..2190)
                     /locus_tag="PKW73_09715"
     tRNA            complement(2111..2190)
                     /locus_tag="PKW73_09715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2154..2156),aa:Leu,seq:gag)
BASE COUNT          540 a          628 c          749 g          682 t
ORIGIN      
        1 agaatttgcc gctcttcacg gctgcctctg gccaatcttc gccagtcgtg agcttcgccg
       61 tccggtgcca ccgctttgta gaattcacta tcgaaggccc gggtgcgcac caccaccggc
      121 atgggtacgg catagcccag taccagagct tgttgcttgg ggtccatctg tgatagtacg
      181 gtgcgcaggg tttgactgcc tgaaacgccg gtgaatacag catcgatatc cttgtcgtcg
      241 ttcaataagg ccgttacccg ggtgcctatc tggctgagaa tctcggggtc gatgccggag
      301 gggcgttgat cgactatgag cagggtgacg aaatacttgc gcatttctct ggcgataata
      361 ccgaatatgg tttgcctcgc cacttgcgga gagagaaatt tgtgagcctc ttcgatgact
      421 ataaccagct tgcgaggcgg attgacgcgt tccggatcgc aaatatagcg ctcggactgg
      481 cgcacatatt cggcgtgaat gcgtctggta ataatgttgg tggcgagcat gtaggaaagc
      541 aggttgttct gcctgccaaa ctccagcaca atgtgtttgc ccgcttgaat ttgctgcagt
      601 atctcttcga tatagttgtg atttaccctc ggtcttagat agggcgtttt ttgcactata
      661 gtattgagcc ggcgttgcag ggtcttgagt gctaccgggt gacctttgtg cacctgggtg
      721 aattcctcga tttcttcgcc ggacatatca aggagggtgg cgatccagtc ctgtcccagt
      781 ttctccctgg ctatgtaggc gttctccagg gtggcctcgg agagtgccag ttcgtttttg
      841 agcaggtcga ggtcttcgat ttctatctgg ttgtagccga tatatagttc tcgggcatcg
      901 ggaatgccgc gagctttgga ggactcggca tcgagggaga agaccaccac ttgatgcccg
      961 aagagttgct tgaggccttt tacccggggg gcgcttttgc tttccgacat ggcttgccag
     1021 ccgtattcgt tgtgcatgtc gaaaaccagt actgatgcca gatctttaga gattatgccg
     1081 ctcaggaaaa tgcggttgag aaaagatttg ccggtgcctg atttgccgaa gatgccgttt
     1141 gatctttcaa tgaagcgatt gagattgacg cagaccggta cctccatgtt gagagggcgg
     1201 ccgatgctga agtaagtatt gcccggtgtg ttatcttcct tgccgaaaac aagggcaaag
     1261 tcttcggcgc tggcttcaaa gacctgggag aaatggctgg gaatagtttt taccggtcgt
     1321 aagccccttc cctcgttttc ttcttcgctt aaagtggcgg cgcttcccag gctgaatgcc
     1381 gccagtgctt gcttttccag catgagcatg ggctgcagtt tgaccgtgcc gaaagtggag
     1441 gtgcccgaca gaactgccat gaggaagggg tcggtgccag gcgggttgaa gagaatgttg
     1501 ggattggagg aggagagggt gacgtcggtg accatgctga agaagcgcgt attggctccc
     1561 tctaccacca caaagcgccc cacgcgcaga tcctccacgc tctgggaagc atccagcctc
     1621 atttcgagcc ccgccgaaag ggagccctgg atgatgatgc ccagatgtga atgcttgctt
     1681 tgctccgtca tggctcttga gcggctattt aattggactg gccaaagccg agggctttcg
     1741 cgaattgatc tacggctatc tgcctgtctt ccagggagta ctcttcccgc ttgactatgt
     1801 cgctttcgtc ttcgccccgg tattcggtga aggtgtagac gatgggctcg tgcaggctgc
     1861 gctccaggcg gtagaggttg ccgtttaagc cgtgggcgcc gcaaaggccc cagaccccat
     1921 caagggcttt ggtgatatcg ctgactgtgt gtggccactg ggctctttgg gtgagcattg
     1981 cttcagacct caaggcggat gatagagaag gatgccgttt gccatcatag cgtaaggcga
     2041 aggacttatg tccttcgcct gtttttcaag ctataagctt aaagtttgct ttctgattag
     2101 aataaaaaat tgtccggaga gggacttgaa ccctcacggt tgcccacacg cccctcaaac
     2161 gtgcgcgtct accaattccg ccacccggac ttgttcagga ggacattgcg tcagcccacc
     2221 ggttgaaaac caggccgact aacagctatt agtttatgca tgttgactat agtgcgtcaa
     2281 cttgcttaga actaagggtt ttgcccatgc tcgcgctttg tcgggcgaaa gtgcctctat
     2341 tgcctggttt tggggcttcg ggttggggct ttgaaaggct taaaactgcg cgaagccagt
     2401 gtcagcctca gttttgataa gagaatttaa cacaagatgt gtggaaattc gtgcaaagtg
     2461 ggcatattta gaatgatccg agccagggaa gtctttctta atgattgaaa tgtttgtctt
     2521 tggaatagct caagatcaga agacgcacat gcctattctg gtgctgtatg acagcaccaa
     2581 aaggcgggct ctccctatt
//
LOCUS       DAOQNZ010000502         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22689_length_2599_cov_3.478381, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000502 DAOQNZ010000000
VERSION     DAOQNZ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22689_length_2599_cov_3.478381"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1490
                     /locus_tag="PKW73_09720"
     CDS             129..1490
                     /locus_tag="PKW73_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73606.1"
                     /translation="MSSNNANPNNGAGSERVENQIGKAADTAPPQLRAEATTLLNQEK
                     PAPQLESPTVVGADGTKRLDFRPQGQDQVVTAYMNGQSDSQLTQGNGKLEVKAPGATG
                     DGTRTDKADKPQAAGSDKGDRLQDGSDRGNKAASDKAIEDMSRTLREGGMSEAEAAKV
                     SKAIHDRLSNVKDGGGAENAETRLKGTPAEQMERMREGFETILKPGQENFSGYNERQR
                     QNVVKDLAYKVASPDDFVNQGNKMTCALQSLEKQHLSAGDPAKVIENAASVLNKGYAD
                     LPGTNQDGSSMRVHVMKQSLTPDSESARDFNTSDHGTGKGMRGLGGQALDALYGQAHA
                     DNRSIRMGQPTSADGFDKAGNIYITAGAQNPPFNMPGSRDATGEALFTRQPDGGLKML
                     HNSPVIGAFDREWLNQTFGGKPGAIMVHENSVGPNNGPDARLGYPPHLDLRLNTFKSP
                     EEL"
     gene            1557..2114
                     /locus_tag="PKW73_09725"
     CDS             1557..2114
                     /locus_tag="PKW73_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73607.1"
                     /translation="MNGQDKLQGHALHAMTARAVEGGLLMDNQNGRSLDRVMNMRQID
                     IATNNKNWNTGDRPQGPAPAPGSKTDAPPAAPMVDEGPRGYRRTPSGSGSDDAYRANQ
                     PVQRHTDGKDPLQKDQKPEDLLNSPLGKDKDKDKKGAADIPMQQKLKALSDYQKALAD
                     YHEAVAKNPNAKVKPPEFKAYLPTE"
     gene            2167..>2599
                     /locus_tag="PKW73_09730"
     CDS             2167..>2599
                     /locus_tag="PKW73_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73608.1"
                     /translation="MLVKELGAIDKILLPPEFAPSNKEYGGRGDNWLLQFSALSSENP
                     EEDVQICFFYRGRVCDQSDTEALRRVTDRSSYSPGNQNLLEADRQEPADHELDLLFSL
                     IDVLGNVGDNQYTRNYTARATGLSGAGFHLQSLELLTVGTRT"
BASE COUNT          724 a          774 c          692 g          409 t
ORIGIN      
        1 ggtcacaaga ccaaagacac cgatgagtcg tacaagcgta ctcaccaggt ttcgcccccg
       61 tcataaatag cgcccgtgac agccgtcaca aattgcctgc tttgccttgt gaggtcaatt
      121 taccaaccgt gtctagtaat aacgccaatc caaacaatgg tgccggctca gaaagagtcg
      181 aaaatcaaat aggcaaggct gccgatacag ccccgccgca actgcgcgct gaagcaacca
      241 ccctgctcaa tcaggaaaaa ccggcccccc aactggagag ccccaccgtg gtcggagccg
      301 acggcaccaa gcgcttagac ttccgccccc agggccagga tcaggtagta acagcctaca
      361 tgaacggtca gtcggacagc cagctcaccc agggcaacgg caaactggag gtgaaagctc
      421 cgggagcaac cggtgatggc accaggaccg acaaagcgga taaaccacag gcggccggca
      481 gtgataaggg cgaccgcctg caagacggca gcgacagggg caataaagcc gcctccgata
      541 aagccataga agacatgagc cgcaccttga gagaaggcgg catgagcgaa gcggaagccg
      601 ccaaggtttc caaagccata cacgatcgac tctccaacgt caaagacggc ggcggcgcag
      661 agaatgccga aactcggctg aagggcacgc cggctgagca aatggaaagg atgagagaag
      721 gtttcgagac cattctcaaa ccgggccagg aaaacttctc aggctacaac gaaagacaga
      781 gacaaaatgt cgtcaaagac ctggcatata aagtagcctc accggacgat ttcgtaaacc
      841 agggcaataa gatgacttgc gccctgcaat cccttgagaa acagcatctt tccgccggcg
      901 atccggccaa ggtaattgaa aacgccgctt ccgttcttaa taaaggttat gccgacctgc
      961 ctggaactaa ccaggacggc tccagcatgc gcgttcacgt tatgaagcag agcctcacgc
     1021 ccgactcgga gtcggcgcgc gatttcaaca caagcgacca cggtaccggt aaaggtatgc
     1081 gcggcttggg cggacaggcc ctggacgctc tctatggaca ggcccatgcc gacaaccgct
     1141 ccattcgcat ggggcaaccc acttccgccg acggcttcga caaggccggc aacatctaca
     1201 ttacagccgg cgcccaaaat ccgcccttca acatgcccgg ctctagagat gctaccggcg
     1261 aagcgctctt caccagacaa ccggacggcg ggctcaaaat gctccacaac agtcctgtca
     1321 tcggcgcctt cgaccgcgaa tggctgaacc agactttcgg gggcaagcca ggcgccatca
     1381 tggtgcacga aaacagtgtc ggaccgaaca acggaccgga tgccagactg ggctatccgc
     1441 cccacctgga cctgcgtctc aacactttca agagccccga ggaactgtag caaaaagttg
     1501 cccagtggga gaaagaaacc ggtcaaccag cccagctcat ggtgcatgcc gcctttatga
     1561 atggtcaaga caaactccag ggtcacgccc tccatgccat gacggccagg gccgtggaag
     1621 gcggcttgct catggacaac cagaatggca gaagcctgga cagagtcatg aatatgcggc
     1681 aaatagacat tgccacaaat aataaaaact ggaacaccgg cgatcggccc cagggcccgg
     1741 ctcctgcccc aggctcaaag acagatgcgc cgccagccgc ccccatggtg gacgagggtc
     1801 cccgcggtta cagacgcaca ccatccggtt ccggcagcga cgatgcctac cgggccaacc
     1861 agccggtgca acgccacacc gatggtaaag acccgctcca aaaagatcaa aagccggaag
     1921 acctgctcaa ctcaccgcta ggtaaagaca aagacaaaga taagaagggc gcggccgata
     1981 ttcctatgca gcagaagctg aaagctctgt ccgactatca aaaggccctg gccgattatc
     2041 acgaagcagt ggccaagaat cccaacgcta aagtgaaacc gccggagttc aaggcctatc
     2101 tgccgacgga atagatagaa gataatagaa agtccctatc cttgaaagaa gaaagaaaac
     2161 tcgctcatgc ttgtgaaaga actgggtgcc atcgacaaga ttctcttgcc gcccgaattt
     2221 gctccgtcca acaaagagta cggcggtcgg ggtgataact ggctgcttca gttcagcgcc
     2281 ctcagttcgg aaaacccgga agaggacgtc caaatctgct tcttttacag aggtagggtc
     2341 tgcgatcaaa gcgacaccga ggccctgcgc cgagtgacgg acagaagctc ttacagcccg
     2401 ggcaatcaaa atctgcttga ggcagaccgg caagagcctg ctgaccatga actggactta
     2461 ctcttctcct taattgatgt actgggcaat gtgggcgaca atcaatacac gaggaactac
     2521 acggctcgag ccaccggtct tagcggtgcc ggatttcacc tgcaaagtct ggaactactg
     2581 accgtcggca ccaggacaa
//
LOCUS       DAOQNZ010000503         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22706_length_2597_cov_3.902439, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000503 DAOQNZ010000000
VERSION     DAOQNZ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22706_length_2597_cov_3.902439"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKW73_09735"
     CDS             complement(<1..384)
                     /locus_tag="PKW73_09735"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA73609.1"
                     /translation="MPPKTAPKQDTKLVLIEAGINIMMEKGYNNTGIQEVLSSVGVPK
                     GSFYHYFDSKEDFACNIIEHFDQCYSNRILESLRDKTISPVKRLKKYCQAVKANLLAQ
                     ECRKGCLIGNLSQEMADQSETLRLCL"
     gene            complement(586..>2597)
                     /locus_tag="PKW73_09740"
     CDS             complement(586..>2597)
                     /locus_tag="PKW73_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73610.1"
                     /translation="ADSVPDLTALAERANAGSQERPASQDQFPKQDLSYWQQALLNMA
                     PALVKDGAISKAAGSGGAGGGKESESLPASVPSESQELTLVQERLAAEKAAGVYGPAT
                     KTLLGDYVKWLESQAIPLEIARRQSYGLEFYPSPSFTRDQTGKVSLAFNADSPSKESL
                     ERVKGAFQWLSANIEDTDKREALLIEATAKQVETSVRDLGFPKGWSRPASEDSSENAL
                     WLKNSMRLLEASLKVREYASLMDDFQKASAGKFPFDLPPGASVERDARGKIVRYNLDL
                     PQSWQLDAAGEAKLAALEKWTEGKRELLEPIKAQLLQLKTLPELVPSNSDLELKNRGV
                     RLDQNGRILSIGERSSGSGSQPGDSSVTANLAVSRYEIEHKDGKVLMHQTVQMKDIPV
                     WGYLNLAGGRDVGKPMQLTREYDPEDYVVVRDSHGASSEYSIVQAKDLQSDGLWNSAR
                     RLGDKALPAAMDVGLFGLGAVEVYGALRSGKPLAGAQGLQLLRGSLHSSVAAAGMFNN
                     AGARETEWGNNLGTARSAYFVAVAGYATAKGSWSLLKSGATASGLTRQALRQSVVSEA
                     GAASGKLAAEMSPALSGQTARSLAVDTASKSSPQLVTEMMASRAKVAGLADKGLGRSI
                     YTGSSALLVGSGALYSPYIVADLVNQSRRVKDDPSRLIERQARKQ"
BASE COUNT          578 a          746 c          644 g          629 t
ORIGIN      
        1 caagcacaac ctcagggtct cgctttgatc agccatttcc tggctcaaat tgccaattaa
       61 gcagccttta cggcattcct gagcgagcaa attagctttg acggcctggc agtatttctt
      121 cagtcgtttc accggcgaaa tggttttatc ccgcagactc tctaaaattc tattgctgta
      181 gcactgatca aaatgctcga taatgttgca ggcaaagtct tctttgctgt caaaatagtg
      241 atagaagctg cctttgggca ctcctaccga ggacaacact tcttgaatgc cggtgttgtt
      301 gtagcctttt tccatcatta tgttgatacc cgcttctatc aaaacgagct tggtatcttg
      361 cttgggagct gtctttggag gcattgaggt tcttccgcat gaaagttggt caacaataaa
      421 ttctctggcg aaaccgttca caagtttcaa attcgatttc cagagccaat gatagagaat
      481 aagaatacaa cagagactct acaccctaga ttagaccggt cgtctgagtt tcgtcaatag
      541 ttttcttgca agtcccggca agtttctgaa aattccctca aaccgtcact gttttcttgc
      601 ctgtcgctca atcaagcgcg aaggatcatc tttgacacgg cggctctgat tgactaaatc
      661 agccacgatg taagggctgt agagagcccc ggaacccaca agcaaagcgg aactgccggt
      721 gtagatgctc cgccccagac ccttatcggc aaggcctgcc accttcgctc ttgatgccat
      781 catttcggtc accagctggg gagagctctt ggatgccgta tcaactgcca gggaacgggc
      841 cgtttgcccg gagagcgcgg gagacatttc ggcagccaac ttgccgcttg ccgccccggc
      901 ctcggagacg acactttgcc tgagcgcctg acgggtcaga ccgctggccg tggcaccact
      961 cttcaaaagc gaccaggaac ctttggccgt agcatatccg gctactgcca cgaaataggc
     1021 cgagcgcgcc gtccccaggt tgtttcccca ttccgtttcc cgggccccgg cattgttgaa
     1081 catgccggcg gcggcaacag aagaatgcaa tgaaccgcgg agcagttgca agccttgcgc
     1141 accggcaaga ggtttgccac tgcgcaaggc accatagact tcaacggcac caagaccgaa
     1201 gagccccaca tccatagcgg caggaagggc cttatcgccc agccgcctgg ccgaattcca
     1261 cagcccgtcc gactgcaaat ctttggcctg cacaatggaa tattcgctac tggcgccgtg
     1321 gctgtctcga accaccacat aatcttccgg gtcgtattca cgggtaagct gcatgggctt
     1381 gcccacatct cgaccgccgg ccaaattgag atagccccaa accggaatat ctttcatctg
     1441 tacggtctgg tgcatcagca ctttgccgtc cttgtgctcg atttcataac gactgacagc
     1501 cagatttgcc gttacggaag aatctcccgg ctgcgaacca ctgcctgaac tgcgctctcc
     1561 aatggagagt attcggccgt tttgatcgag cctgactcca cgattcttca gctcaagatc
     1621 ggaattactg ggcaccagtt cgggcagggt tttcagctgc aatagctgag ccttgattgg
     1681 ctccagaagc tcccttttac cttccgtcca cttctcgagg gcagccagtt tcgcttcccc
     1741 tgccgcatca agctgccagg actgaggcag gtcaaggttg tagcgaacta ttttgccccg
     1801 ggcatctctc tccacagagg ctcccggagg taaatcgaaa gggaacttac cggcggaagc
     1861 tttctgaaag tcatccatca aactggcata ttcccttact ttcagggaag cctccagcaa
     1921 tctcatggag ttttttagcc agagagcgtt ttcggatgaa tcctcagaag caggtctgga
     1981 ccaacctttt ggaaagccca gatcgcgcac ggaagtttcc acctgtttgg cggtggcctc
     2041 aattaataga gcttctcttt tgtctgtgtc ttcgatattg gccgaaagcc attgaaaggc
     2101 acctttgaca cgctccaggc tctccttgga aggtgaatcg gcattgaaag ccagggagac
     2161 cttgccggtc tgatcacggg tgaaactggg gctcgggtaa aattccaggc catagctctg
     2221 tcgcctggcg atttctagag gaatggcctg actttcaagc cacttcacat aatcaccaag
     2281 caaggttttc gtggcaggtc catagacacc ggcagctttc tcggcggcca atctttcctg
     2341 cacaagcgtc agttcctgac tttcggaagg caccgatgcg ggcagagact ctgattcttt
     2401 tccaccgcca gctccgccgc tcccagccgc tttactgata gcgccgtctt taaccagcgc
     2461 cggtgccata ttgagcagtg cctgctgcca ataacttaga tcctgcttcg gaaactgatc
     2521 ctgacttgca ggccgctcct gactgccggc attggctcgt tccgccaggg ctgtcagatc
     2581 aggcacggag tctgccg
//
LOCUS       DAOQNZ010000504         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22722_length_2596_cov_3.929949, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000504 DAOQNZ010000000
VERSION     DAOQNZ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22722_length_2596_cov_3.929949"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1036)
                     /locus_tag="PKW73_09745"
     CDS             complement(173..1036)
                     /locus_tag="PKW73_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73611.1"
                     /translation="MKLVLLGVGKLALSVARLAHESGVVQSVYGTTRSAKRFAELRDS
                     GIEPLHFYQLFGGGPGGLAREFSAQQVAEFHELFEDACLLISFPPSGDFSYPAAFAQL
                     CRNRARSVIYISSTSVYGSQTGLIADSTDVCADTPLAAARLRAECFFREAGAIVLRAP
                     ALYGFDRGLHLRLLSGSYVLPPLADNIVSRIHQDDLGAIILSAFNKLAAGECYLVGDK
                     LPARHRDVFLWLCERLKLDPAGFAGKNAGGTIRADRSVDGATILKRLEYELRYPDYKM
                     GYEAILSRLGR"
     gene            complement(1033..2052)
                     /gene="cobD"
                     /locus_tag="PKW73_09750"
     CDS             complement(1033..2052)
                     /gene="cobD"
                     /locus_tag="PKW73_09750"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNA73612.1"
                     /translation="MQHGGNLVEAERLFGIDRNQWLDLSTGISPLAYPLSPLPAGLWQ
                     RLPLENSALLSAAAGYYHCSFLLPVSGSQWAIQTLPRLRQGCRVAVAGPTYAEHAQCW
                     QRHGHEVQAVQTVSREQLLTASKSVDVLVLANPNNPTGTLHEKDFLLALYENLREHQG
                     WLLVDEAFMDFEDAQSLLPLVESLQAEGLLVLRSPGKIFGLAGARLGFVFAWPELLHL
                     LSEEQGPWALPAPCLEVFRPAFLDRSWQRHTFAELESLSGRLRALLSSYGLQDSAGTT
                     LFCYVETSLAPVIYREMAAQGVLLRLFESGSPALRIGLPGCEPDWQRLNSALASLTCL
                     QGCVL"
     gene            complement(2027..>2596)
                     /locus_tag="PKW73_09755"
     CDS             complement(2027..>2596)
                     /locus_tag="PKW73_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HNA73613.1"
                     /translation="SRDTASLNEEGVAVAAVESLLENGSDAIFGALFWFALAGAPGAL
                     FYRLVNTLDAMWGYRNERYSDFGWAAARLDDLLNFIPARLTAFTYALCGDSEMSLHCL
                     REQGRTWYSPNAGPVMAAGAGALNLSLGGDAVYGGKVKSRPVLGSGPRPCAEDIKRAV
                     KLVKKSLKVWVCLSVLGGVLAIATRRQSR"
BASE COUNT          673 a          785 c          693 g          445 t
ORIGIN      
        1 ccatgtcccg gccgaatacg aagagatagt cagccattct cttaagcccc ggcgcgaaga
       61 gaaagccgcg aaagtgctgc cggaccatct tcttacggcc gtagccgccc gctatgacga
      121 aatcctaaat cacatttagt tattccggca gcgaccttaa agcgaagttc tactaacggc
      181 caagcctgct caaaatagcc tcgtagccca ttttatagtc tgggtatcga agctcgtatt
      241 ctagtcgctt caaaatagtt gcaccatcca cggagcggtc ggcgcgaata gtgccgcccg
      301 cgtttttacc ggcaaagccg gcaggatcaa gcttgagccg ctcacaaagc cagagaaaca
      361 catcccgatg ccgggccgga agtttatccc cgaccaaata gcattccccg gctgcgagct
      421 tgttaaaagc agagaggata atagccccca gatcgtcttg atgaatacgg gagacaatgt
      481 tgtcggccag aggcggcaag acataggagc cggagagcag ccgcagatga aggcctctat
      541 caaagccgta gagcgcaggc gctctcagca cgatggctcc ggcttctcga aagaagcact
      601 ccgcccgcag gcgcgccgct gcaagtggcg tatcggcaca gacatcggta ctatccgcaa
      661 taagtccggt ctgtgaacca tagacactgg tggaggaaat gtaaataacg cttcgggctc
      721 tattgcggca caattgcgca aaggcagccg gatatgaaaa atcgcccgag ggaggaaagc
      781 tgatcagcag gcaggcatcc tcaaagagtt cgtgaaattc ggcaacctgc tgcgctgaga
      841 actccctcgc caaacccccg gggccacccc cgaacaattg atagaaatgc aaaggctcga
      901 tgccggaatc ccgcagttcg gcaaagcgct tcgcactgcg agtagtacca tacacggatt
      961 gaacgacacc gctctcatgc gcaagccgag ccacgctcag ggccaacttt cctacaccaa
     1021 gcagcaccag cttcacaaga cacagccttg caagcaggta agagaggcaa gcgccgaatt
     1081 taagcgctgc caatcgggct cgcagccggg aagcccaatg cgcaaagccg gagacccgct
     1141 ctcgaataag cgcaataaaa caccttgagc cgccatctcc ctatagataa ccggcgccag
     1201 agaagtttcc acatagcaaa agagagtcgt gcccgcgcta tcttgcaatc cgtaagaaga
     1261 aagcagcgca cgtaggcgtc cactcaagct ctcaagttcg gcaaatgtat ggcgctgcca
     1321 ggagcgatcc agaaaagccg ggcggaatac ttccagacag ggagcgggca gagcccaggg
     1381 tccctgctct tcagacaaca agtgaagcaa ctccggccag gcgaagacaa agcccagtcg
     1441 cgctccggcc agtccgaata tctttcccgg cgaccgcaaa actaaaagtc cttccgcctg
     1501 caaagactcc acaagaggca gaagactctg agcatcttcg aaatccatga aagcttcatc
     1561 cactagcagc cagccctggt gctctctcag attttcatag agcgcgagaa gaaagtcttt
     1621 ctcatgcaag gtgccggtag gattatttgg attggcaagc accagcacat ccacgctttt
     1681 gcttgccgtg agcagttgct cccgacttac agtctgcaca gcctgcactt catgcccatg
     1741 acgctgccag cactgagcat gctctgcata agtaggaccg gccaccgcca cccggcaacc
     1801 ttgacgcaac cggggcaggg tctgtatagc ccactgtgaa ccggacacag gcaataggaa
     1861 agagcaatgg taatagccgg ccgcagcaga cagcagcgcg ctattttcga gcggcaggcg
     1921 ttgccagagc cctgccggaa gcggcgaaag cggataggca agcgggctta tgccggtgga
     1981 gaggtcgagc cactggttgc ggtcgatacc gaaaagtctc tccgcttcaa cgagattgcc
     2041 gccgtgttgc aattgccagc acccctccca gaaccgaaag acagacccag actttaagcg
     2101 acttcttcac cagcttcacc gcccgcttga tgtcctcggc acagggacga ggaccgctac
     2161 cgagcacggg ccggctcttg actttgccac cgtatacggc atcaccaccc aggctcaaat
     2221 taagggcacc ggcgccggca gccataaccg ggccggcatt ggggctgtac catgtgcgcc
     2281 cttgctcccg caagcagtgg agcgacattt cgctgtctcc gcaaagggca taggtaaaag
     2341 cggtcagtct ggccggtata aagttgagca gatcgtcaag cctggccgcc gcccagccaa
     2401 agtccgagta gcgttcattg cgataacccc acatggcatc gagagtattc acgaggcgat
     2461 agaagagcgc gcccggcgca ccggccaacg caaaccagaa caaggcacca aaaatggcat
     2521 cactgccgtt ctcaagaagc gactccacag cggccacggc cacaccctcc tcattgaggg
     2581 aggctgtgtc acggct
//
LOCUS       DAOQNZ010000505         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22784_length_2592_cov_3.470635, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000505 DAOQNZ010000000
VERSION     DAOQNZ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22784_length_2592_cov_3.470635"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PKW73_09760"
     CDS             complement(<1..900)
                     /locus_tag="PKW73_09760"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73614.1"
                     /translation="MFCYQCEQTSQGTGCNTMGVCGKGPECAALQDLMVHICKGISMY
                     AHRARKLGAKDAYIDTKVMEALFMTLTNVNFDTEEHIDYIMDMAATLEKARGLYTEAA
                     RAAGLPVEVLTGPAAYTPVRDRLALLTFAETLNIMKRISKENQDVVSLQELVTYGVKG
                     LAAYAHHAFVLGYVDERVSAFIYEAFDYLSQPEQTVDALLGYALKTGEINLLVMEMLD
                     RAHTETFGHPEPTVVRTTPVKGKCIAVSGHDLNSLHQVLLQTQGKGINVYTHGELLPA
                     LAYPLMKKFPHLVGNYGTAWQNQV"
     gene            complement(1155..1589)
                     /locus_tag="PKW73_09765"
     CDS             complement(1155..1589)
                     /locus_tag="PKW73_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA73615.1"
                     /translation="MKVLIAVDDSKFSQKIGDFVVRQAFPAGTEFVVLSVVENMLVGS
                     YMSVLPSPMLEEIKAAARTTCHDTLEKLAARIAEHYPAAAVKQELLEGFPKEEIIEYA
                     ASWGADLIIVGTHGRKGLSRFVMGSVSQAVCQSAPCSVLLVR"
     gene            1662..>2592
                     /locus_tag="PKW73_09770"
     CDS             1662..>2592
                     /locus_tag="PKW73_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNA73616.1"
                     /translation="MHGLINWLTIAMRIVTMQPTIQVKSPISAPGAMPVQEVLEHFGS
                     DRQSGLTSQKVLEHRQTFGLNAMTKRQGVSAFTLAVRQFQSTVVILLLVASAVSFASK
                     EHIQAWGILIAVLINALVGFATELKAKISLESLEAMAGPTARTVRDGKEIDLPASELV
                     PGDVVILDSGDRVPADLRIIEASSLSADESAMTGESESVYKTEEPGPDNPPESTQLFQ
                     GTLVLSGRGRAIVYATGNNTKLGQLGILLTEAASGRTPLENGLEELGKQLTLLTVVLC
                     TVIAGIGVWHKENIFLMLQTAVALAVAAIPEGMP"
BASE COUNT          660 a          714 c          654 g          564 t
ORIGIN      
        1 cacctgattc tgccaggcag taccatagtt tccaaccaga tggggaaact tcttcatgag
       61 cggataggca agagccggca gaagttcgcc atgggtgtag acattgatgc ccttgccctg
      121 ggtctgcaaa agaacttggt gcagagaatt caaatcgtgg ccggaaacgg caatacactt
      181 acctttgaca ggtgtagtgc gcaccacggt aggctcagga tgtccgaagg tttcggtgtg
      241 ggcccggtcc agcatctcca tgaccagcaa attgatttcg ccggttttga gtgcgtagcc
      301 aagcaaagca tctacggtct gctccggctg actcaagtag tcgaaagctt catagataaa
      361 ggccgatacc cgctcatcta cgtaacccag gacgaaagca tggtgagcat aggcagccag
      421 gcctttcacg ccgtaggtaa ccagttcctg cagagagacg acgtcctgat tctctttgga
      481 gattctcttc attatattga gggtttcagc aaaggtcagc agagccaatc tgtcccgcac
      541 cggagtatag gcagccggtc cggtcaaaac ctcaaccggc agtccggcgg cccgtgccgc
      601 ttccgtatag agaccgcgag ctttttcaag agtggcggcc atatccatga tgtagtcgat
      661 atgctcttcg gtgtcgaagt taacattggt gagagtcatg aagagagctt ccatgacttt
      721 ggtatcgatg taagcatctt tggccccaag cttgcgggct ctatgggcat acattgaaat
      781 gcccttgcag atatggacca tcaaatcctg caaggctgca cattcgggac cttttccgca
      841 aacacccatt gtattgcaac cagttccctg ggaagtttgc tcgcactgat aacaaaacat
      901 actcataact tttcctcagt caccgaataa ccaaaaaact tgctctggaa tgtagcaagt
      961 gcattaacag caaaacaagc atggcagaaa gccttgactc tatcttcagc aaaacgacct
     1021 agtccgaaag gctgagcaag ccccagggaa gtcaggcaga acgagccccc gacaacgagt
     1081 taagcgaact ggaaagtaat ggcgagaaag tagaattaag gatgaattta tccgaattag
     1141 ccggactgca aaaactagcg cactaaaagt acggaacagg gcgcagactg acatacggcc
     1201 tgcgaaacgc tgcccatgac aaaacgactt agccctttgc gcccgtgggt gcctactatg
     1261 attaaatcgg caccccagct tgccgcgtat tcaattatct cttccttcgg gaacccttcc
     1321 aaaagctcct gtttcacagc ggcagccgga taatgctcgg cgatccgggc agccagtttc
     1381 tccagggtgt catggcaagt ggtcctggca gccgccttga tttcctcaag catgggagaa
     1441 ggcagcacac tcatgtaact tcccacgagc atgttctcga cgacgctcaa aactacaaac
     1501 tcagtacctg caggaaatgc ctgcctgacc acaaagtcac caatcttctg agaaaatttg
     1561 ctgtcatcga cggcgatcag tactttcact tgaggctctc tttcccacaa tggagagtta
     1621 cttatctaga ttgattcaca attctagcag ttaagacata attgcatggt ctaattaact
     1681 ggctgaccat agccatgaga attgtaacca tgcaaccgac catccaggtc aaatctccca
     1741 taagcgcccc tggcgctatg cctgtgcaag aggtgctcga acatttcggc agcgaccggc
     1801 aatcgggatt aaccagccag aaggtgctgg aacaccggca gaccttcggc ttaaatgcca
     1861 tgaccaaaag gcaaggggtg agcgccttca ccctggctgt caggcagttc cagtccacag
     1921 ttgtcatact gcttctagtg gcgtcagccg tctcctttgc ctctaaagaa catatacagg
     1981 cctggggtat cctgattgcc gtccttataa acgccctggt gggctttgcc accgaactga
     2041 aagccaagat ttccctggaa agcctggagg ccatggccgg accaaccgcc cgaacagtgc
     2101 gggacggcaa agagatagac ctgccggcct cggagcttgt gcctggtgac gtggtcattc
     2161 tggacagcgg cgatagagtt ccggccgacc tgcgcatcat cgaggcctcc tccctttcgg
     2221 ccgacgaatc ggccatgacc ggtgagagcg aatctgtcta caaaacagaa gagccgggcc
     2281 cggacaatcc ccccgagtcc acccaacttt ttcagggcac cctggtctta tcgggccgcg
     2341 gcagagccat cgtctatgcc accggcaaca ataccaaact tggtcaactg ggtatcctcc
     2401 tgactgaggc agcatccgga cgtacccccc tggaaaacgg actggaggag ctcggtaagc
     2461 aattaacgct cctgactgtg gtgctctgca cagttattgc cggcatcggt gtctggcaca
     2521 aggaaaatat ctttctcatg ctgcaaacgg cggtagccct ggctgtagca gccattcccg
     2581 aaggcatgcc cg
//
LOCUS       DAOQNZ010000506         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22864_length_2587_cov_4.105450, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000506 DAOQNZ010000000
VERSION     DAOQNZ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22864_length_2587_cov_4.105450"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="purB"
                     /locus_tag="PKW73_09775"
     CDS             complement(<1..823)
                     /gene="purB"
                     /locus_tag="PKW73_09775"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNA73617.1"
                     /translation="MIERYTRPQMGDIWSLESKFQTFLEVELAVMEAQCAMGLVPAQA
                     LAEVKEKARFDVKRIDEIEAEVRHDVIAFLTNISENVGESARFVHMGLTSSDVIDTAL
                     ALQLKKASSILLDDLDKLTETVLQQARKHKRTVMVGRSHGIHAEPITFGFKLAVWVEE
                     LRRHRLRLEQAIEMIAVGQMSGPVGTFSNISPEVEERALKILALTPAKISTQVIQRDR
                     HAQFMTTLALIAASLEKFSVEIRHLQRTDVLEAEEPFHAGQKGSSAMPHKRNPVAS"
     gene            1140..2063
                     /locus_tag="PKW73_09780"
     CDS             1140..2063
                     /locus_tag="PKW73_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA73618.1"
                     /translation="MKILLAIDVAQSTNKDEAKAARELVNIKSLAKLLPLDKSQVTLL
                     YVKEELPSFEQVLKAQADFPEDLTHVVERRAGKILDILKKGVEQEGASVNSEIVSGPP
                     AQLIETVARDLGADVVVLAQSKHHDSIFLSRVSHHVARHSPVSTILLREGADEDSLDK
                     TGAKTVVIGLDGSKNCKESVIKALKLLNLDKEKTNLILTYVVNVSKIIASVTPFSFVT
                     AMQENLMMEGEVFLADTQKELSTLGYKKLSIHLKAGRPDAEIIKLLDEKGGNLVVIGA
                     QGGGAIKHFLMGHICERVISQAPVSTLVARG"
     gene            2071..>2587
                     /locus_tag="PKW73_09785"
     CDS             2071..>2587
                     /locus_tag="PKW73_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNA73619.1"
                     /translation="MKHQFLLRSLSLMLIFLPLCQPAARAQFNEVDDLAAEQAKEKLE
                     LEDMRKELGSIKGEVKDALRSMSELTNAANTRLPSNAAVHEIHLFAKEAEVDIGGGQK
                     FRALTYNGQIPGPEIHVREGEPTKIVLHNELGQPTSLHFHGIPLPHGVDGLPRAGKPV
                     SSTRGEEETGPK"
BASE COUNT          667 a          722 c          657 g          541 t
ORIGIN      
        1 cactggccac cggattcctt ttgtgaggca tggcggaaga gcctttctgc ccggcatgga
       61 aaggttcctc tgcctcgaga acatcagtgc gctgcaagtg cctgatttcc acggagaatt
      121 tctccaggga agcggctatc aaggccaggg tggtcatgaa ctgagcatga cggtctcttt
      181 gaatcacctg ggtggaaatt tttgccggcg tcagcgccag tattttcaaa gcccgctctt
      241 ccacttccgg agagatattg gaaaaggttc ccacaggacc gctcatctgc ccaacggcaa
      301 tcatctcgat ggcctgttcc agacgcaggc gatggcgcct cagttcctcc acccagacgg
      361 ccagcttgaa gccgaaggtg atgggctcgg catgaatgcc gtgggagcgg cccaccataa
      421 ccgtacgctt atgtttgcgc gcctgctgca agacagtttc ggtcaactta tccagatcgt
      481 cgaggagtat gctggaagcc ttcttgagct gaagagccag agccgtgtcg atcacatcgg
      541 aactggtcag acccatgtgc acgaagcgcg ccgactcacc gacattctca gaaatattgg
      601 tcagaaaggc gatcacatcg tggcgcactt ccgcctcgat ttcatcgata cgcttaacgt
      661 cgaaacgcgc tttttctttt acttccgcca gagcctgagc aggcacgagg cccatggcac
      721 actgcgcttc cataacagcc aactcaactt ccaaaaaggt ctgaaactta ctttccaggg
      781 accatatgtc gcccatctgc gggcgtgtat atcgttcaat cacggcggac tcctcttttc
      841 cttcgaacta ttgtccctga agatagctat caagcggtgc acccggcaaa gaaaattctc
      901 aaggtttatc tttctccatt tagatgtgag tcaatttaaa taaacctact taaatcaacc
      961 tgccgccccc cccctttgca agctacacaa acgccacccg ctcaaattgt ggcgctcacg
     1021 caccggtagc gatatcaaga acagccccca gaaacgaata agacggggca aagaggttag
     1081 aatataggcg ctgacatatg tgacaaagcg ggaacgacct tgtctcgatc ggggatatca
     1141 tgaaaattct acttgccatc gacgtggctc agagcaccaa taaagatgaa gctaaagccg
     1201 cccgggagct tgtaaatatc aagagcttgg ccaagctttt gcctctcgat aaaagccagg
     1261 tgacgctact ctacgtaaag gaagaattgc cctcctttga gcaagtactg aaagcccaag
     1321 ctgacttccc cgaagatctc acccatgtgg tggagcgccg ggccggcaaa attctggaca
     1381 ttctaaaaaa gggagtcgag caggaagggg cttcggtaaa cagcgaaatc gtctcaggcc
     1441 ccccggcgca attgattgaa actgtagccc gcgacctcgg tgccgatgta gtagtgctgg
     1501 cacaaagcaa gcaccacgac agcatctttc tcagccgcgt cagccaccat gtggccagac
     1561 acagccccgt atcgaccata cttctgcgcg agggcgcaga tgaagacagc ctggacaaaa
     1621 ccggtgctaa aaccgtggtg attggtcttg acggctccaa gaactgcaag gaaagtgtga
     1681 tcaaggcgct taaattgctc aacctggaca aagaaaagac caatttgatt ttgacttatg
     1741 tggtcaacgt ctccaaaatt atcgcctccg tgactccttt ctcctttgtt acggccatgc
     1801 aggaaaacct gatgatggaa ggcgaagtct tcctggccga tacgcagaaa gaactctcta
     1861 ccctgggtta caaaaagctt tccatacatc tgaaggccgg tcgtcccgat gccgaaatta
     1921 tcaaactgct tgatgagaaa ggcggcaatc tggtggtaat cggggcccag ggcggcggcg
     1981 ccatcaaaca cttcctcatg ggtcatatct gcgagcgggt catctcgcaa gcaccggtat
     2041 caaccctcgt ggcaagaggc tgacagcggc atgaagcatc agttcttgct caggtctctc
     2101 agcctgatgc tcatctttct tcccctctgc caaccggctg cccgggctca gttcaacgag
     2161 gttgacgatc tggcggctga gcaggccaaa gaaaaactcg aactggaaga catgcgcaaa
     2221 gaactgggct ccatcaaagg cgaagtcaaa gatgccctgc gctcaatgag cgaactgacc
     2281 aatgccgcca acaccagatt gccttccaat gccgccgtgc acgaaattca cctctttgcc
     2341 aaggaagcgg aagtggatat aggcggcggg caaaagttcc gggcgctgac atacaacggt
     2401 caaattcccg gtcccgaaat tcatgtaagg gaaggagaac ccaccaaaat tgtgctccat
     2461 aacgagctgg gacagccgac ttccctgcat ttccatggaa taccgctgcc ccacggcgta
     2521 gatggtcttc cgagggcggg caaacctgtc agcagtacca gaggcgaaga ggagaccgga
     2581 cccaagc
//
LOCUS       DAOQNZ010000507         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22868_length_2587_cov_3.577409, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000507 DAOQNZ010000000
VERSION     DAOQNZ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22868_length_2587_cov_3.577409"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..469
                     /locus_tag="PKW73_09790"
     tRNA            397..469
                     /locus_tag="PKW73_09790"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:430..432,aa:Ala,seq:cgc)
     gene            complement(548..>2587)
                     /locus_tag="PKW73_09795"
     CDS             complement(548..>2587)
                     /locus_tag="PKW73_09795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA73620.1"
                     /translation="IYQIIDVIGSGGAGIVYRAEHIMLNQQHALKVLLPAAHTAENWR
                     RFEQEARALAKLDHASIVKVFNMGFDSNRYPYFSMELVDGISLSDYLQQHGKLAPEAA
                     IEVFLQLTDALRQAHKLGIVHRDIKPGNIMLLEKGKREGTRQAGFAVKLLDFGIARLV
                     DDRRTQEKTAAGAVFGTPLYMSPEQGRGEPVDSRSDLYSLGCAFFETLTGRPPFHGGS
                     ILETIDLHQRGQLPSLGSAGLVSDLTDELDICMQRLLAKEPQRRYQSAEQFAHDLLRI
                     KDGKSVSPSILGSFDKSLSFLVTGSGDAQGMRVRLITLAVVLVMALGLIASLISSASK
                     TGEPIRAETNLTNPLNLNQSEHLKQSENLNQSEHLKQSENLNQSEHLNQSENLKQSEH
                     LHQSENLNQSESLHQAAVPSRKGRYYKGIVSEGGRKYYHFLFPDNDAYVGKLAVFGGG
                     AAKFPIGEVKLPAEGSLLVWLSNGGEDGLEDARCYTGSPIHGLTVSDVDVARLVEIIR
                     PWKELTYLRIRTAGLTAADVDALSTMTRLEGFSLENLERRKGDVDVAALAKSSLLGRL
                     NVLELSQLQSVQPLLPSLRKSRTIERLHIVGCQLSESDLKEILKLPGLKYLDIDQRKM
                     PAAQTLKALQGTNLKSLRLIVGGWTKADEAALKSINSGYVYRPWKERNDSSSYLN"
BASE COUNT          657 a          703 c          602 g          625 t
ORIGIN      
        1 catggtcaag ataaacaagg cagctagttt ctgtgtaatc ttggaagaca cgttcatgaa
       61 agacacctgt caataaatta gaggttctta tactactgcc agccctcagc ccggtcaaat
      121 ttccttatca tttggctccc cttttagctt gacaagccag tcgaaacagc cccaggcaaa
      181 gaattcccga tcggaaggaa tcctgaccga aagccccccc atccaagaga aactatgacc
      241 taaagaaatc ccaaccgaaa taaacacctc gcgggaggac ttataaactc tctcgggcgc
      301 ggaggttcta agccgggatt tatgagaatt tgagaatttg tagagcctag tctgccctaa
      361 gagtgggtgg tctggaataa gatatatgca tcctgagggg atatagctca gttggtagag
      421 cgcacggatc gcacccgtga ggccaggggt tcgaatcccc ttatctccat ttttttaatg
      481 aagagtctct catcagtgac gtatacaacc taggaccggg ctaaaccgct agcttttcac
      541 cctgctccta gttcaggtaa gatgagctat cattgcgctc cttccagggg cgataaacat
      601 aaccgctgtt tatactcttg agagcggcct cgtctgcttt tgtccagccg ccgacaatca
      661 gtctcaggct cttcaggttg gttccctgca aagccttcaa agtctgagcg gcaggcattt
      721 ttcgctggtc aatatcaaga tatttcagac ctggtaattt caaaatctct ttcagatcgc
      781 tttcagaaag ttgacagccg acaatatgca gacgctcgat ggtcctgctc ttacgcaggg
      841 acggcagcag aggttgcacc gattgcaatt gtgacaactc caatacattc aaacgaccaa
      901 gcaagctcga tttagccaga gcagctacat ccacatcgcc cttccgacgt tcaagatttt
      961 ccagactgaa gccctctaaa cgtgtcatag tggagagcgc atcgacatcc gcagcggtta
     1021 atccggcggt gcggattcgc aaataagtaa gctccttcca gggtctgatg atctccacca
     1081 aacgcgccac atctacatct gaaaccgtca gcccgtgaat gggactaccg gtatagcagc
     1141 gggcatcctc caggccatct tcaccaccat tactcagcca tacaagcagt gagccttcag
     1201 caggcagttt cacctcaccg attggaaatt tagcagcccc gccgccaaat acggccaact
     1261 tgccgacata agcgtcatta tcgggaaaga gaaaatggta atactttcgc ccgccctcgg
     1321 agacgatccc tttatagtat ctcccctttc tggagggtac agcagcttgg tgcaagctct
     1381 ccgattggtt caagttctcc gattggtgca agtgctccga ctgtttcaag ttctccgatt
     1441 ggttcaagtg ctctgactgg ttcaagttct ccgactgttt caagtgctcc gattggttca
     1501 agttctccga ctgtttcaag tgctccgatt ggttcaagtt caatggatta gtcaaattgg
     1561 tttctgctct gataggctca cctgttttag atgctgaaga aatcaagctt gcaatcaagc
     1621 ccagagccat aaccagaaca acagccagcg ttatcagtct gactctcatt ccctgcgcat
     1681 caccactgcc ggtgaccaaa aaagacaagg acttgtcgaa agaaccaaga atggagggcg
     1741 aaacactctt gccgtccttg attcgcaaca aatcatgggc aaactgctcg gctgactgat
     1801 atcgccgctg cggctccttg gccaataggc gctgcatgca aatatccagc tcatcggtca
     1861 gatcactgac aagaccggca ctgcccaggg agggaagctg acctctttga tgaaggtcaa
     1921 tggtctccag aatactaccg ccatgaaagg ggggacggcc tgtgagggtt tcgaagaagg
     1981 cacagcccag ggaatagaga tcgctgcgac tgtctacagg ctcgccgcgc ccctgctccg
     2041 gactcatata gagcggagtg ccaaagactg caccggcagc tgttttctcc tgcgttcttc
     2101 gatcatccac caacctggca ataccaaaat cgagaagctt gaccgcaaaa ccagcttgcc
     2161 gtgtgccttc tctcttacct ttctcaagca gcattatatt gccaggctta atatctcgat
     2221 gcacgatacc tagcttatga gcttgccgga gcgcgtctgt cagttgcaag aacacttcga
     2281 tagcagcctc aggagcaagc tttccgtgct gctgcaaata atcactgagg ctgataccat
     2341 ccaccagctc catggaaaag tacgggtagc gattgctatc gaaacccata ttgaatacct
     2401 tgacgatgct ggcatgatcc agcttggcca gagcccgcgc ttcctgttcg aagcgcctcc
     2461 agttctcggc agtatgcgct gccggcaaga gcactttgag cgcgtgttgc tggttgagca
     2521 taatatgctc ggctctatat acgatgccgg ctccaccact gcctatgaca tcaattatct
     2581 gatagat
//
LOCUS       DAOQNZ010000508         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22924_length_2583_cov_3.650316, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000508 DAOQNZ010000000
VERSION     DAOQNZ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22924_length_2583_cov_3.650316"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /gene="mnmG"
                     /locus_tag="PKW73_09800"
     CDS             complement(<1..1605)
                     /gene="mnmG"
                     /locus_tag="PKW73_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNA73621.1"
                     /translation="MWKTGFKMYFDVVVIGGGHAGCEAANASARLGLSTMLLAVNLDT
                     IGAMPCNPAVGGPGKTHLAREVDALGGVMGEATDATYLQIRMLNSSRGPAVQALRAQS
                     DKREYAIWMKNYMESLPNLTLRQGMAKSLIIEDGKVAGVELNFGDRINCRAVVLCAGT
                     FLEGTIWIGKETMPAGRAGEFPAVGLSAYLRSLGFSTGRLKTGTPPRIDGRTIDYSKL
                     PEVPGDAHLQFFSFLDKRPVLPQIPCHQTRTTERTHELIRNNLHQSPMYSGMIHGIGP
                     RYCPSIEDKIVRFADKDSHSLFLEPEGRSTYEVYLQGCSTSLPVQVQHDIVHSLPGLE
                     NASMVRPAYAVEYDYMPAIQFTHALMAKDLPGFFAGGQILGTSGYEEAAAQGIVAGIN
                     AALYVKGEAPFELPRSSSYAGTLIDDLVTKEIGEPYRMMTSRSEYRLLLRQDNADVRL
                     TPLGREIGLVDDYRWRLFNEKQEAIGAEKTRLKKTRIHPGQGFEEALLPFDESLKQSV
                     TLEELLRRPRVPYSVIEAKAPASEVPF"
     gene            1865..>2583
                     /locus_tag="PKW73_09805"
     CDS             1865..>2583
                     /locus_tag="PKW73_09805"
                     /inference="COORDINATES: protein motif:HMM:NF041379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HNA73622.1"
                     /translation="MLSMPPTREPARRSLRPKQVVSLNLVTLCSLALVLLMALSTVQI
                     ARAESNAASQNATDKPASGSLLIKTVLDPEKYFGKAKLGYMAARLCPEICEKLFCYCG
                     CDVTDEHSTLLDCFTSDHGVDCYICQEEALIALKMKREGKSLAEIQKAIDLQYEKQNP
                     FAPTDKLLRYQKERLWQPEKTSSPGKTKGKQSEKLAGQTTTNANSAKSKRGLPSASSR
                     DSSGTLPSENSQSEKQTQGNSR"
BASE COUNT          666 a          734 c          677 g          506 t
ORIGIN      
        1 aaagggcact tcagaggccg gcgccttggc ctcgatgacc gaatacggca cccggggacg
       61 gcgcaatagc tcttccagag tgactgattg cttgaggctt tcgtcaaagg gcaaaagcgc
      121 ttcctcgaag ccctgaccgg ggtgaatccg ggttttcttc aacctggtct tctcagcgcc
      181 gatggcttcc tgcttttcat tgaatagacg ccatctgtag tcatcaacca gaccaatttc
      241 acgaccgaga ggagtcagtc tgacgtcggc attatcctga cgcaaaagca gacgatactc
      301 agaacgagag gtcatcatgc gataaggctc accaatctcc ttggtgacca gatcatcaat
      361 gagggtgccg gcatagctcg aagaacgagg caactcaaag ggtgcctcac ccttcacata
      421 gagagcggcg ttgatcccgg ccacgatgcc ctgggcggcg gcttcttcat agccggaggt
      481 accgagaatc tggccgccgg caaagaatcc gggcaggtct ttggccatca aggcgtgggt
      541 gaactgaatg gccggcatgt agtcgtactc cacggcataa gccggtctga ccatggaggc
      601 gttctccaaa cccggcagag aatgcacgat atcgtgttgc acctgtacag gcaaactggt
      661 ggaacaaccc tgcaaataca cttcataggt ggaacggcct tccggctcca gaaagagcga
      721 atggctgtcc ttatcggcaa agcgcacgat tttgtcttcg atggaaggac aatagcgcgg
      781 tccgatgccg tgaatcatgc ccgagtacat gggcgactgg tgcaagttat tgcgaataag
      841 ctcatgggtg cgttcggtgg tgcgggtctg atggcaggga atctggggca aaaccggacg
      901 cttgtccagg aaggagaaaa attgcaaatg agcatcgccc ggcacttcgg gcaatttgga
      961 ataatcaatg gtacgaccat caattcgagg cggcgtgccg gttttgagcc gcccggtact
     1021 gaaaccgagg gaacgcaaat aggccgaaag gcccacagcc ggaaattctc cggcccgacc
     1081 ggcgggcata gtttctttgc cgatccagat agttccttcc aggaaggtac cggcgcacaa
     1141 cactacggcg cggcaattga tgcggtcacc gaaattcagt tccacacctg ctactttgcc
     1201 atcttcaatg atcaaacttt tggccatgcc ctgacgcaaa gtcagattcg gcaatgattc
     1261 catgtaattt ttcatccaga tggcatactc gcgcttgtcg gactgggcgc gcaaggcttg
     1321 cacagccggt ccacgactgg aattgagcat gcgaatctgc agatatgtgg cgtccgtggc
     1381 ttcgcccatg acgccgccga gagcatcaac ttcacgggcc agatgcgtct tgcccggtcc
     1441 acccacagcc ggattgcaag gcatagcacc gatggtgtcc agattgacgg ccaggagcat
     1501 agtggaaaga cccagacgcg cactggcatt tgccgcctca cagccggcat gtccgccacc
     1561 gattacaacc acgtcgaaat acatcttgaa acccgttttc cacaagctgg aaaaaccatc
     1621 ataacaaatg ctcttgataa acccctaaac ctctccaaag cctaatcttt tcacttccgg
     1681 ccaggcagtc tgccggccct cattaaaggc gggccgagta ggaccaaatc ttgtggcgga
     1741 gagcatttca gaaactgaaa cgaaaaaccc cgagagcaag ccggggctgc cccggcggtc
     1801 tatactttgt tacatagata agttacctcg ataaagcgcc accgcactac ccgtgagatt
     1861 cccgatgttg tccatgcccc caaccagaga accggcccga agaagcctcc gacccaaaca
     1921 ggtagtttcg ctcaacctgg tcacattgtg cagcctggca ctggtattgc ttatggcctt
     1981 aagcacggtc cagatcgccc gggctgaatc aaacgccgct agtcagaacg cgaccgataa
     2041 gccggcttca ggcagccttc tgatcaagac agtgctcgac cctgaaaagt acttcggtaa
     2101 agcgaaactg ggctatatgg ccgcccgact ctgccctgaa atctgtgaaa agctcttctg
     2161 ctactgcggc tgcgacgtca ccgacgaaca ttccaccctc cttgactgtt tcacctccga
     2221 ccatggcgtc gactgctata tctgtcagga agaagcactc atagctctaa aaatgaagcg
     2281 tgaaggcaag tcccttgccg agattcaaaa ggccatcgac ctgcagtacg agaagcaaaa
     2341 tcctttcgcc cccaccgata agcttctgcg ctaccagaaa gaacggctgt ggcagccgga
     2401 gaagacaagt agccccggca aaacaaaagg taagcagtcc gaaaaattag cggggcaaac
     2461 cacaactaac gctaactccg ctaagagcaa aagaggcttg ccaagcgcat ccagcagaga
     2521 cagtagcggc acgttgccaa gcgaaaattc gcagtcagag aagcaaaccc aaggcaactc
     2581 tcg
//
LOCUS       DAOQNZ010000509         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_22979_length_2580_cov_4.263762, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000509 DAOQNZ010000000
VERSION     DAOQNZ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22979_length_2580_cov_4.263762"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKW73_09810"
     CDS             <1..492
                     /locus_tag="PKW73_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73623.1"
                     /translation="QSNDLASRYQLEYHHSSLWKNTLASIAQRRLLEAANANHAARSR
                     GGQRQLDYYGGRIGHGNEVSDMAMINGYWALSDFAVQSYRARAEAARKAALVAQSAAD
                     LKAQMLQPVSPGGPSLRALGTNLYVRYYGNENSSIVDPPAPDDPVVELAAQAKKLKPA
                     PGK"
     gene            642..1403
                     /locus_tag="PKW73_09815"
     CDS             642..1403
                     /locus_tag="PKW73_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73624.1"
                     /translation="MNAADREKCCPSKTAKEGVAVSTCSQPGRLESARELLAEGKDFK
                     LNASQTSVSMQDAIKKARGLSGQAGTLSTNLKKAPPGALDQYKRDLGAFSSHVGAYKA
                     HLEQVEKELGHCKSSEKAYAEHLRKYSLHTDQFHIPNIPPPHICEELQISEAENARLA
                     NQLKIDRERLAQAEMSLAESEAQLKDSLRQNQHADQALLKRSRMLEEERKLAGEFETL
                     KTELDLLNSQHRALTGGKTTPSLGLGKVSGKVKGK"
     gene            1515..2051
                     /locus_tag="PKW73_09820"
     CDS             1515..2051
                     /locus_tag="PKW73_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73625.1"
                     /translation="MSTTPAALQFLGRHLSVGLLCKSGAALRRNSAMALLLAGALSLP
                     LAEKAFAQSSPYPQALPAVSTGSVDFNTVDCPFTRSAGGNGAFGGSSRPGSGNGGRGG
                     AMPALPPGWIGVQNTHTGEYMGAIPPGGNLVDFFRGAYGIEPGQEAEAAWILENQLPQ
                     AYESGMFDGMGVWGPGGP"
     gene            2205..>2580
                     /locus_tag="PKW73_09825"
     CDS             2205..>2580
                     /locus_tag="PKW73_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73626.1"
                     /translation="MGHQYGRDPVERESNIVADQLDNNDAYGAANRLRQDLNNMYPED
                     FNRLVNRTMQKDEKGYGADLVIVNQSAGRDNCGRQITERVLGVQGYDQWNRAQFYPVT
                     SLGENRSQYRCDGGQYYPPVPLP"
BASE COUNT          614 a          696 c          731 g          539 t
ORIGIN      
        1 caatccaacg acctggcttc acgctatcaa cttgaatatc accattcttc cctctggaaa
       61 aatacactgg ccagcatcgc tcaaaggcgg cttctggaag cggccaacgc caaccatgcg
      121 gcgcgcagcc ggggcggtca gaggcaactt gattattacg gcggccgcat cggccatggt
      181 aatgaagtca gcgatatggc catgataaac ggctactggg ccctttccga ttttgccgtg
      241 caaagctacc gggcccgggc ggaagccgct cgtaaagccg ccctggtagc ccagagtgcc
      301 gccgatttga aagcgcagat gctgcagccg gtgagcccgg gcggtccgag cctgagagcc
      361 cttggtacca acttatatgt gcgctactat ggcaatgaaa attccagcat cgtcgacccg
      421 cctgcgccgg acgatccggt ggtggaactg gctgctcaag ccaagaagct caagccggct
      481 cctggtaagt aacaggcaga taaggatcag gcaaaattgc aaggagaggt aagttgaccc
      541 cggtcaaatc ttggttgaga ttcactttag cattgagcag cactgcattt tcactggctc
      601 tgcagtccac tttcacttct ttagctgcag cggcggaaga cgtgaatgct gcggaccggg
      661 aaaagtgttg cccgtccaag accgccaagg aaggcgtcgc agtctctacc tgctcccagc
      721 ccggtcgtct agaatctgcc cgagagttac tggctgaagg caaagacttt aaattgaatg
      781 ccagccagac cagtgtcagt atgcaggacg ccatcaagaa agcccgcgga ctgtcggggc
      841 aggccggcac tctcagtacc aatttgaaga aagcgccgcc tggtgcactc gatcagtata
      901 agcgtgatct gggagctttc agttcccacg tgggcgccta caaagctcac ctggagcagg
      961 tggagaagga actcgggcat tgcaaatcaa gcgaaaaagc ctacgctgag cacctgcgca
     1021 agtatagttt gcacaccgat cagtttcaca ttccaaatat accgccgccg cacatttgtg
     1081 aagagttgca gatttccgaa gctgaaaacg ccaggctggc caatcagctc aagatagata
     1141 gagaaaggtt ggcccaggcg gagatgagcc tggcagaaag cgaggcccag ttgaaagaca
     1201 gcctcaggca aaaccagcat gccgaccagg ctttgctcaa gcgcagtcgc atgcttgaag
     1261 aagaacgcaa gctggccggt gaatttgaaa ctttgaaaac cgaattagac cttctgaaca
     1321 gccagcaccg tgccctcacc ggtggcaaaa ctacgcccag cctcggtctt ggtaaagtga
     1381 gcggtaaggt aaaaggcaag tagaagggca agcagcatct gaaaagattt gccaaaattt
     1441 tgccaggaat ttgaccgccg gctgccgggg gctgaggcta aattggcttc atcaataaga
     1501 tggagtttga accaatgagt accacccctg ccgcccttca gtttcttggc cgccacttgt
     1561 cggtaggctt gctttgcaaa tcgggtgcgg ctctgcgacg caattccgct atggctttac
     1621 tacttgctgg cgccctttct ctgcccctgg cagaaaaggc ctttgcccag tcttcaccct
     1681 atcctcaggc gctgcctgct gtttccaccg gttcggttga cttcaacacc gtagattgtc
     1741 cttttacacg ctcggccggc ggcaatggtg cttttggcgg ctcttccaga ccaggcagcg
     1801 gcaatggcgg tcgcggcggt gccatgccgg ctctgccgcc gggctggatt ggcgtgcaga
     1861 atacccatac cggtgaatac atgggggcga ttccgcccgg gggcaatctg gtggatttct
     1921 tccgcggcgc atatggcatt gaaccggggc aagaagctga ggctgcctgg attctggaga
     1981 atcagctacc ccaggcctac gaaagcggta tgtttgacgg catgggcgtc tggggaccgg
     2041 gcggaccata gagcctgaga cgagtgtcag cctgcttctt gacaagggat gtcggcttgt
     2101 gatttattca atcttctacg tatttttacc gatgacagag ggtatatata gtggcatcat
     2161 gatcgagcaa gccctcgaaa agtaatttgt tggagtttct aaatatggga catcagtacg
     2221 gccgcgatcc tgtagagcga gagtcaaaca tagtcgccga ccagctcgac aacaacgacg
     2281 cttatggcgc tgccaatcgt ctgcggcaag acctgaacaa tatgtatccg gaagatttca
     2341 atcgcctggt caatcgcacc atgcagaagg acgagaaagg ctacggtgcc gacttagtca
     2401 tagttaacca gtcggccggg cgcgataact gtggtcgaca gatcacagag cgagtgctgg
     2461 gcgtgcaagg ttatgaccag tggaatcggg ctcagtttta tccggtcact tccctgggcg
     2521 agaaccgctc tcagtacaga tgcgacggcg gtcagtatta cccgcccgtg ccgctaccgg
//
LOCUS       DAOQNZ010000510         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23063_length_2576_cov_3.267751, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000510 DAOQNZ010000000
VERSION     DAOQNZ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23063_length_2576_cov_3.267751"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKW73_09830"
     CDS             complement(<1..877)
                     /locus_tag="PKW73_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73627.1"
                     /translation="MPRNDDHYMRNRGVRKPRAPYGDYSSADDQGSEDMEPARAPDNG
                     ARSNRQDSFRASRGAPASFRELDRKAREEAEEEKEKKRKKDRKRLFNLLSNEALCLAI
                     IFAMIATFNYMSSDYTGEYLTSNPQLGLVKLSLLRRAASVEGELCYGRYPNMQMISGV
                     DPGKPNQRMEFSTSQEWQEAGNLPHKAVFLGTISSSAAVGTLVDARGKYKLKLEKNMV
                     ASVFRQFQAHLPVLPTFQPPTWVKTEKPLIAPAKPGFSREEQIRQLQLPALRKQLEEE
                     HLLKETPAKQPGKRGV"
     gene            complement(894..1769)
                     /locus_tag="PKW73_09835"
     CDS             complement(894..1769)
                     /locus_tag="PKW73_09835"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNA73628.1"
                     /translation="MLPAAIKTPYQAGKSSKVGRFFIRLAWLYLALTLVQFAASPALA
                     QRRPAGKPQTQSSGPRPYPEFRSRFGVIRWLSDQMPLKVFVSRGLSLDSIMDPQLGAP
                     VANVDNLAAWPNLVAQIVENPQQWNSLGVAEGYVDGHYQAALEGVSAWKAFEPEGFLS
                     YQITNDPQEADIYVFFTKQFVNKLGMALFANDIRGYTSKTNFPYKAILAGGQAAFKPV
                     VIVLRTTEARGASMPLGKMKAAATHEFGHALGIEGHSTNAADLMSVYYGRGVISPNDA
                     ATLRYLYRLQPDLIP"
     gene            2194..>2576
                     /locus_tag="PKW73_09840"
     CDS             2194..>2576
                     /locus_tag="PKW73_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNA73629.1"
                     /translation="MVAVRAIILAAGQGKRMKSAKPKVLHEVLGMPILSRVVRQCQAL
                     APEKLHVVVGHQAELVRDFVEKTFEDGSCLTHLQEPQLGTGHAVMQVAPTLEGFNGHV
                     IVTVGDAPVIQSATLKHLLLSHEQSG"
BASE COUNT          529 a          661 c          727 g          659 t
ORIGIN      
        1 tgaccccgcg cttgcctggc tgtttggccg gcgtttcttt cagcaagtgc tcttcttcca
       61 attgcttgcg caaagcgggt agttgcagtt gcctgatttg ttcttccctg gagaaaccag
      121 gcttggccgg cgcaatcagc ggtttctcgg tctttaccca ggtggggggc tggaaggtgg
      181 ggaggaccgg cagatgggcc tggaattgcc gaaacaccga tgccaccatg ttcttttcca
      241 gtttgagctt gtacttgccg cgggcgtcta ccagggtgcc tacggcggcg ctactactga
      301 tagtgccaag gaagacggcc ttgtgcggta aattgcctgc ttcctgccat tcttgagagg
      361 tggagaattc catgcgttga tttggtttcc ccgggtctac gccgctaatc atctgcatat
      421 tcggatagcg cccataacag agctctccct ccaccgatgc ggcacgccgc aaaagcgata
      481 gtttaaccag ccccagttgc ggattcgatg tcaggtattc gccggtgtaa tcactgctca
      541 tgtagttgaa ggtggcaatc atggcaaaga taatggccag gcagagggct tcattgctga
      601 gcaggttgaa gagccgcttg cggtcttttt ttcgcttctt ttccttctcc tcttccgctt
      661 cttccctggc ttttcgatct aattcccgaa agcttgcagg agccccgcgc gatgcccgga
      721 agctatcctg gcgattggaa cgtgcgccgt tgtccggtgc ccttgccggt tccatatcct
      781 cgctgccctg gtcgtctgca ctggaataat caccataagg agcccggggc ttgcgcacgc
      841 ctcggtttct catgtagtga tcatcgtttc tgggcatttt tcgcctttgg cactcagggg
      901 attaaatccg gttgcagtcg atagagatag cgcagtgtgg ccgcgtcgtt tggcgagatg
      961 acgcccctgc cgtagtacac gctcatcagg tcggcagcgt tggtgctgtg tccctctatg
     1021 ccgagggcat gaccaaactc gtgagttgca gcggctttca ttttgcccag gggcatagaa
     1081 gcgcctctgg cttcggtagt gcgcaagacg atcaccactg gtttgaaggc ggcctgccca
     1141 ccggcgagaa tggccttgta tgggaaattt gtcttggaag tgtaacccct gatgtcattg
     1201 gcgaaaaggg ccatgcccag tttgttgaca aactgcttgg tgaagaagac gtagatgtca
     1261 gcctcctgcg ggtcattggt gatctggtaa gaaagaaagc cttccggttc aaaggctttc
     1321 caggcgctga ctccttccag ggcggcctga taatgtccgt ccacatagcc ttccgctacc
     1381 cccaggctgt tccattgctg gggattttcc actatttgcg ccaccagatt gggccaggct
     1441 gccaggttgt cgacgttggc aacgggcgct cctaattgcg ggtccatgat ggagtcgagg
     1501 ctcaaaccgc gactgacgaa gactttaaga ggcatctggt cggaaagcca gcgaattact
     1561 ccgaaacggc tgcgaaactc cggatagggt cttggaccgc tcgactgggt ctggggcttg
     1621 ccggccggtc gtctctgggc cagggccggt gaagcggcga actgaaccag agtcaaggcc
     1681 agataaagcc aggccagtct gataaaaaat ctgccgactt tagagctttt tccggcttgg
     1741 tatggcgttt tgatagctgc tggcaacatt ttgctgacct tttcgtctac ttccgatatc
     1801 ttaagcccga gtctctagct atatgccaca tactcactca agagctctga ctaaaagtag
     1861 atgataatct ggtttctatt gttatgcatt ttcgttacag gcaaaataaa cgaaaggtta
     1921 aaaggcaggt gtctcaaggg ctttcaggta agaattagca tgttgatatc gcagtcactc
     1981 cagtagagag gcacaaggga ttgagcctgt tggggcagca ccaaccctgc gagcattttt
     2041 agaggctccc tactcaggga gcctcgtttt ttctatgctt ttaaacatta gagcctgtcc
     2101 agttcttagc acgaggcagg ctcttgtgtt aacctaactt gtcacagacg atagtttttc
     2161 aagagagccg catagcggcg ctcaggagta gttatggtag cggtgagagc aattattttg
     2221 gctgctggac agggcaagcg catgaagtcg gccaagccca aggttttgca tgaggtactc
     2281 ggcatgccga tcttgagccg ggttgtaagg cagtgtcagg ctctcgctcc cgagaaactg
     2341 cacgtggtgg tcggccatca agccgagctg gtgcgcgatt ttgtcgaaaa aacttttgag
     2401 gacggctcct gtctcactca tttacaggaa ccgcagttgg gtaccggcca cgccgttatg
     2461 caagtagcgc ctacccttga gggctttaat ggtcatgtaa ttgtcaccgt gggagatgca
     2521 ccggtcatac agagtgccac gctaaaacac ttgctcctaa gtcatgagca gtccgg
//
LOCUS       DAOQNZ010000511         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23093_length_2574_cov_3.864629, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000511 DAOQNZ010000000
VERSION     DAOQNZ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23093_length_2574_cov_3.864629"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKW73_09845"
     CDS             complement(<1..568)
                     /locus_tag="PKW73_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73630.1"
                     /translation="MYRYYDWLVEFHDDFGLERSRGISKADLTQFKATASVGVEDFAK
                     AKEALAFFKPSAPGNKTPDFAKFDLDGDAALSDEELRKASNVGRSVGDAAMLQYLMKE
                     RQENGALGRAETPTINEAAVEKLAREKVAAFSINSEIDELSRRLDKSLAALKPEATES
                     YSYPDQGQIGDCYLVAAVASIMRYEPALL"
     gene            complement(1048..>2574)
                     /locus_tag="PKW73_09850"
     CDS             complement(1048..>2574)
                     /locus_tag="PKW73_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNA73631.1"
                     /translation="TALIKAMAKDTDLQKQLSGWKTLQLEETLTGRHPNGSPGFADDN
                     MPLTGEAEKVFTIYLREDNNLEDTVRKLDSIISREGVGARTETGATATSTRMGSSNRI
                     TVTRDVLERSPDSTTSHPLALVDTPLIQAIKDRYGVAKNQRLGDTLLRKLEVTCGLRG
                     DSLGYDTQGNLVLKAQVQSDDNYHGGVYLNDTAKHSSSTFGDYTDRQAYYALARSFNL
                     DPINISPRRSVNSGQQETPATKGADTGKRINIPVVTDLTRGAEIRAFAPDNQLVKLYT
                     AYSPSVARIWNQGRTPQSPTINSGSGFFITDSTGRPSRLVATAHHVIKEYSSSNLYIE
                     VADMLNAPPYLDNQRVLKPGQHTARVIKSDPDLDLAILELDQNRAGIGYRSYPAISGD
                     AVREARPQEYIFPFGTIRNFNQVISGVGQVGKVGPLGTLFSNSEMPPSRLYESRQARV
                     MAGETTILKGMSGGPTFSEDGALLGVNSISRTTQDPKFHESVFPTAEQLFKLLDSRQF
                     "
BASE COUNT          528 a          624 c          689 g          733 t
ORIGIN      
        1 ttaacagagc tggttcgtag cgcataattg aggctaccgc cgccaccagg tagcagtcgc
       61 cgatttgtcc ctggtcagga taactatatg attccgtagc ctccggctta agcgccgcca
      121 gtgatttatc cagccttcgc gaaagctcat cgatttcgga gttgatggaa aatgccgcca
      181 ccttctcccg ggccaatttt tccactgctg cctcatttat agttggagtt tcggcccgac
      241 ccaaggcccc attttcttgt cgctccttca tcaagtattg aagcatggcg gcatcgccta
      301 cagaacgtcc gacattggat gccttcctga gttcctcatc tgaaagagcc gcatctccgt
      361 ctagatcgaa cttagcaaag tccggcgttt tgtttcctgg agcggagggt ttaaagaatg
      421 ccagcgcttc ctttgccttc gcgaaatcct caacaccgac tgaggcggta gccttgaatt
      481 gagtcaggtc tgctttgctt atgccgcggc ttctctcaag cccgaagtcg tcatggaact
      541 caaccagcca gtcatagtag cggtacattg cggcaagagc taacgcatct tgccccttga
      601 tattattgtc ttgtaaggca gttgagagtt ctcttttgcc tagaaagtca ttgccgtcac
      661 tgtctagtct ttgaaaaagt tgaatagatc gcagcgcggc cttctcagac gctgtcccct
      721 tcgtctcatc tatttcaatt tgcggcagga cttggctctt ttggagctct ctttgcgcat
      781 tccgggtagc ttcgtcggaa cttgacagat gagctactcc aatgtcggtg tcttggaaga
      841 gttttaattc cgtttggctg aggttatgtt cgcttttctg ctcactgcgg tctggatttt
      901 ccatggtctg gtcttccgat ttcaagaaaa tgaggattgc ttgccgaatt aaaatgatgc
      961 ccggagtaac tgcggaccgg aagtgacctt agtaatttga ttgctgttcg ggcctcttca
     1021 gaagggtgtc ccaaattaca gcgctactca aaactggcgg ctgtccagta gtttgaagag
     1081 ttgctcggca gttggaaaga cggattcatg gaattttgga tcttgcgtag ttcggcttat
     1141 ggagttgacc ccaagtaagg cgccgtcttc actgaatgtc ggtccgccgg acatgccttt
     1201 caagattgtt gtttcgccgg ccattacccg ggcttgacgg ctttcataaa gacgacttgg
     1261 aggcatttcg gaattggaga atagggtgcc cagtggtccg actttaccga cctggccaac
     1321 gccggagatt acctgattaa agtttcgaat ggtgccgaat ggaaaaatgt actcttgtgg
     1381 tctggcttct ctaaccgcat cgcctgagat tgctggatag gagcggtagc ctatcccggc
     1441 tctgttctgg tctagttcca atattgccag atctagatcc gggtctgatt tgatgactct
     1501 ggccgtatgt tggccaggct ttagaacacg ctggttgtcg agataaggcg gcgcgttgag
     1561 catgtccgcc acttcgatgt acaggttgga ggatgaatat tctttgataa cgtggtgcgc
     1621 ggtggcaact agccgagaag gtctgccggt agagtctgta atgaagaagc cgcttcccga
     1681 gttgatagtg ggtgactgcg gggtgcgtcc ttgattccag atgcgggcta cacttggact
     1741 ataagctgta tacagtttga ccagttggtt gtcgggcgca aaagcgcgaa tttccgcccc
     1801 tctggtcaga tctgtgacaa ccggaatgtt gattctcttg ccggtgtcag ccccttttgt
     1861 cgcaggtgtt tcttgctgcc ctgaattaac cgaccggcgt ggtgagatgt tgattggatc
     1921 aagattgaag cttcgagcca gagcgtagta tgcttgccgg tccgtgtagt ctccgaaagt
     1981 actggaggag tgtttggccg tgtcattaag atagactcct ccatggtagt tgtcgtccga
     2041 ctgaacctgt gcttttaaga ctagattacc ttgagtgtcg tatccgagac tatcgcctct
     2101 aagaccacat gtgacttcca gttttctaag cagggtgtca ccgagccttt gattctttgc
     2161 tacaccataa cggtctttga tggcttgaat gaggggcgta tctactagcg ccagcggatg
     2221 agacgtcgtt gagtcgggag agcgctccag cacatcgcga gtaaccgtga tgcgattgct
     2281 gctgcccatt ctggtagagg tggctgtagc tccggtttca gtcctggcac ctactccctc
     2341 acgggagata attgagtcga gctttcttac ggtatcctca aggttgttgt cctctctgag
     2401 ataaatggta aatacttttt ccgcttcgcc tgtaagcggc atgttgtcgt ctgcaaagcc
     2461 cggcgaccca ttaggatggc ggcccgttaa agtctcttcc aattgcaagg ttttccagcc
     2521 ggagagctgc ttttgcaagt cagtgtcttt agccatggcc ttgataaggg ccgt
//
LOCUS       DAOQNZ010000512         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23219_length_2566_cov_3.982079, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000512 DAOQNZ010000000
VERSION     DAOQNZ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23219_length_2566_cov_3.982079"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKW73_09855"
     CDS             complement(<1..252)
                     /locus_tag="PKW73_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73632.1"
                     /translation="MTTCKEAVVSTLGSLAKVFDRTRPFRDASNGGRFWMAGNTFLVA
                     LDAMYAASVRDDYGFAVEALSFFKDSLKDTAPSGWRDDNG"
     gene            complement(667..1269)
                     /locus_tag="PKW73_09860"
     CDS             complement(667..1269)
                     /locus_tag="PKW73_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73633.1"
                     /translation="MSKIENTSLTPTLVEELGNGLLEIYNLPTDEAFLRELFRDLFLN
                     HWQEIQYGILVQGGVLEFEPPCAPTKFGYLDGYLTVEFGQFGHLHLCVGWNVGTGCVP
                     SSEAVSRLRLPSRVELYRKLNSRHEPTYWGLRCFNSREPDAEQTMHIYLPNPLLTKEM
                     QLAAEPDWSRLALWDYLRATYLSLPEPDPKDRLAKRFTHD"
     gene            complement(1418..2464)
                     /locus_tag="PKW73_09865"
     CDS             complement(1418..2464)
                     /locus_tag="PKW73_09865"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HNA73634.1"
                     /translation="MLDSLRELLKSPGLKQRLIFIAALIFAFHAAVIFLLLSHASSYP
                     TLLGLALLAYGLGLRHSVDPDHIAAIDNTTRKLMQEGKKPVGVGFFFALGHSSIVIVM
                     CMLVAVSASFVNDMLPVYKETGVLVSSLASCFFLLAIGFINLFVLVDTFKTWRKLVRG
                     DYPHDLTLEEYLSTGGLLASILKPVLNMVGTSWSMYWVGLLFGLGFDTASEVALLGMS
                     GSTGVSSMPLSVILVIPLAFTVGMTLIDTLDGILMLGAYGWAFLKPARKLYYNVTITF
                     TSVMIALFIGGIEGLQIISKHTGATGWLWDFANGLELENWGFYIIGIFIVSWTASMLL
                     YRLRGYDSLTERKP"
BASE COUNT          713 a          635 c          715 g          503 t
ORIGIN      
        1 accattatcg tcacgccagc cgctgggagc agtgtctttc agactatctt tgaaaaaaga
       61 cagcgcctca acggcaaagc catagtcgtc cctgacagag gcggcgtaca tggcatcgag
      121 ggccaccaga aaagtattgc cagccatcca gaaacgaccg ccgttggaag catcgcgaaa
      181 aggtctggta cggtcaaaga ccttagccag actgcccagg gtggatacta ccgcttcctt
      241 gcatgtggtc attatgtttc ctttcaaata acgggcaagt gcccaatggc aacatgggca
      301 cttatttcat ggatagaatt ctaggagttt caagtactca tttgcaagcc agccgaagga
      361 aatcaagatc aactcagcca ttcaatctgt tcgcttgtat agccgaccgg tctattattc
      421 tgtcgcaaaa gaaaatagtg gcagttgtgg aagcgttcag gagacagcaa aagaacacgg
      481 aagtacagcg agaaagagaa attgaacgtg aaacagaaag aagaagaaga agaagaagaa
      541 gaacacgaga aaaaagagga ggcatccatg cctcctccac aagcgctcta gcaccaatca
      601 caaagtcggc gcagtcgcga ctgactgacc gactgactga ctgagccgac cattagactc
      661 cggtgcttaa tcgtgggtga agcgcttagc gaggcgatct ttgggatcag gctcgggcag
      721 cgacagatag gtggcgcgca gataatccca gagagccagg cgactccaat caggctcggc
      781 agccaactgc atttctttgg tgagcagcgg gttgggcaga taaatgtgca tggtctgctc
      841 ggcatcgggc tcacggctat tgaagcagcg caatccccag taagttggct cgtggcgcga
      901 attgagcttg cggtagagct ctacccgcga aggcagccgc aaacgagaga ccgcctctga
      961 gcttggtacg cagccggtac caacgttcca acccacacat agatgcaggt gtccgaactg
     1021 gccgaattct acggtcaagt agccgtccag atagccaaac ttagtgggcg cgcagggcgg
     1081 ttcaaattcc agtacaccac cttgcacaag gatgccgtac tgaatttcct gccagtgatt
     1141 gaggaagaga tcgcggaaga gttcccgcaa gaatgcttcg tcggtgggca ggttgtagat
     1201 ctccagaaga ccgttgccga gttcttcaac gagggtggga gtgagggaag tattttcgat
     1261 tttgctcatg gttttaccct ggtcaaaagg ttcacaggca ccggatggtg cccgtgtttg
     1321 cggatgaaag aaagaggacg gcgccgcagg actgagcccg ggcgcggttt ctcttaatcc
     1381 agaaagtaag ggtcttcgcc ctcggcgaga tatctgccta aggcttgcgc tcagtcaggc
     1441 tgtcatagcc gcgcaggcgg tagagaagca tggaggcggt ccaactgact atgaaaatgc
     1501 cgatgatgta gaagccccag ttttcaagtt cgagcccgtt ggcaaagtcc cacagccaac
     1561 cggtggcacc ggtatgtttg ctaatgattt gcaagccttc gatgccgcca atgaataacg
     1621 caatcatcac ggaagtaaaa gtgatggtga cgttgtaata gagcttcctg gcaggcttga
     1681 gaaaagccca accgtaggcg cccagcatca gtatgccgtc gagggtgtca atcagagtca
     1741 taccgaccgt gaaggcgagc ggaatcacga gaatgacgga aagcggcata ctgctcacgc
     1801 cggtggaacc ggacatgccg agcaaagcca cttcgcttgc cgtgtcgaag ccaagaccga
     1861 acaagagtcc gacccagtac atgctccaac ttgtgccgac catattcagc accggtttca
     1921 gtatgctcgc cagaagcccg ccggtgctta aatattcctc cagagtcagg tcatgggggt
     1981 agtcgcctcg caccagtttg cgccaggtct tgaaagtgtc caccagcaca aagaggttga
     2041 tgaagccgat ggctagaaga aagaagcagg aggccagcga agaaaccagc acgccggttt
     2101 ctttgtagac aggcagcata tcgttgacga aagaggcgga gacagccacc agcatacaca
     2161 tgacaatgac gatgctggaa tgtccgaggg caaagaaaaa gcccacaccc acaggtttct
     2221 tgccttcctg catgagtttg cgagtggtgt tatctatggc ggcaatgtga tcgggatcca
     2281 ccgaatggcg cagacccaat ccgtaagcaa gaagcgccag accaagcagt gtgggatagc
     2341 ttgaggcatg ggacagaagt aagaaaatga cagctgcatg aaaagcaaag atcaatgcag
     2401 caataaatat caagcgttgc ttgagtccag gtgactttaa gagttcgcgc aaactatcga
     2461 gcataaaggc tccggttcag ttaaatcaaa agtcagtagt caaggcatgc ttaaccggaa
     2521 atcaatgaca agcgcttgca ccctggtcaa ccacagtgtg ccgcaa
//
LOCUS       DAOQNZ010000513         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23220_length_2566_cov_3.888491, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000513 DAOQNZ010000000
VERSION     DAOQNZ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23220_length_2566_cov_3.888491"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKW73_09870"
     CDS             <1..705
                     /locus_tag="PKW73_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73635.1"
                     /translation="RKAHSLNHAGAEKTTSAKAKCTIFLDLNPEYIDMHQGTFTGGWL
                     TPRQLDECRGFVSRLLIELDAAKHPLQLVLHEKIVAELSPVVSKCKNLKIIGHPSLPP
                     AEHHKLRNTADLVLLRASRCVGAAQTALSMVPALHTICPTARDYMGESYSAAIAEDLG
                     IACCLNHEGFSLREGIMDYLDSGKAAQVAQRAQEMAISTWQERGPDFVLNLLYSTPKS
                     ESKHTGRQGWNQEICA"
     gene            783..>2566
                     /gene="sulP"
                     /locus_tag="PKW73_09875"
     CDS             783..>2566
                     /gene="sulP"
                     /locus_tag="PKW73_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HNA73636.1"
                     /translation="MTAPSQNSHLNPFKREAQPHGLGRWVPGLKLLRTYKRRWFLRDL
                     SAGAVLTTILVPVGMGYAEASGLPAICGLYATMTALVTYAIFGRSRILVLGPDSALVA
                     IIAASVLPLAARGSAHAVELAGVLAILAGVMCVAAGLLRLGFITDLLSKPIRLGYLNA
                     IALTVILGQLPTLLGFKVKGLDVLTEASGIVKGIAGNQVNHTAFAIGGTCLLIILACK
                     RWLPKFPGVLLAVFLATVVVSLFNLAHQTGLSVVGEIPRGLPSWRVPEVSEKELNLLF
                     HSAIAIALVSFADMSVVSRTFALRGRYETDDNKELFALGLCNVASGFFQGFSVTGSAS
                     RTPVAEAAGAKTQLTSVTGAICVALLIVIFPSLLMNVPHTALSAVVICACLSLVDISS
                     VIELYGVRKAEFVACLACFLGVAVAGVIPGIFIAVGISLLVFIIRASRPYSAVLGRAK
                     GLKGYHDISRHPEAKQIPGLVLFRWDAPLFFANARLFRECALEAIANAPTAVKRIVIE
                     AEPVTDIDSTAADALRELEHDLNAVSIELGFAQMKGPVKDQLKNYGLFQKIGEENFFP
                     TLGVAVDAYVHTHQIEWHDWDGKKCCKHA"
BASE COUNT          562 a          702 c          709 g          593 t
ORIGIN      
        1 agaaaggcac attctttaaa ccatgctgga gcggaaaaga cgacctctgc taaggctaag
       61 tgcacaattt ttctagacct caatccggaa tatatagaca tgcaccaggg caccttcacc
      121 ggcggctggc tgacacccag gcagcttgat gagtgtagag gctttgtcag tcgattgctg
      181 atcgagttgg acgcagcaaa acatcctctg caactggttt tgcacgagaa aattgtggca
      241 gagttaagcc ccgtggtaag taagtgcaag aacctcaaaa taatcggtca cccttcactg
      301 ccgccggcag agcaccacaa gctgagaaac accgctgacc tggtcttact gagagcgtcg
      361 cgttgcgtgg gagcagcaca aacagcgcta tcgatggtgc cggctctgca tacaatctgc
      421 ccgaccgcca gagactatat gggagagagc tatagtgctg ccattgcaga agacctggga
      481 atcgcctgct gtctaaatca tgaaggtttc agcctgcgcg agggaataat ggattacctt
      541 gactccggaa aagccgcgca agtagcccag agagcacagg aaatggccat cagcacctgg
      601 caggagcgag gaccggactt tgtcctgaat ctcttataca gtactcccaa gtcggagtcg
      661 aagcacactg gaagacaggg ctggaatcaa gagatttgcg cctgaaactg tcaggttttg
      721 cttgacctcc tgtataatgc cacggcttga ggtaattgtt caggcagcaa cttcagggtc
      781 cgatgacagc tcccagtcaa aacagtcacc taaatccctt taaaagggaa gcccagcccc
      841 atggtctggg gcgctgggtg ccggggctga agctcttacg cacctacaaa cggcgctggt
      901 tcttaagaga cctatcggcc ggagcggtgc tcaccactat tcttgtgcct gtaggcatgg
      961 gctacgccga agcatcgggt ctgcccgcta tctgcgggct ctacgccacc atgaccgccc
     1021 tggttactta tgccatattc ggtagaagcc gcattctggt tctgggtccg gattctgccc
     1081 tggtggcaat tatcgcagcc agcgtcttgc ccctggcagc gaggggctcg gcccatgcgg
     1141 tggaacttgc cggggtgctt gccattcttg ccggagtgat gtgcgtagca gccggacttc
     1201 tgaggctggg cttcatcacc gaccttttat caaagcccat tagactcggt tatctcaatg
     1261 ccatcgccct caccgtcatt ctcggtcagc ttccaacact gcttggcttc aaggtgaagg
     1321 gtctggatgt gcttaccgaa gcaagcggca ttgtgaaagg tatcgccggc aatcaagtta
     1381 accatactgc cttcgccatt ggtggcacct gcttacttat tattctggcg tgtaagcgct
     1441 ggctgcccaa gtttccgggg gtgctgctgg cagtctttct ggccactgta gtggtatcac
     1501 tcttcaatct ggcccatcaa accggactct cggtggtggg agaaattccc cgggggctgc
     1561 cctcctggcg cgtgcccgag gtgagcgaaa aggaattgaa tttgctattt cacagcgcca
     1621 tagctattgc gctggtgtca ttcgccgaca tgtcggtggt atcacgcact tttgctctgc
     1681 gtgggcgcta tgaaaccgat gataacaaag agctttttgc ccttggtcta tgcaacgttg
     1741 caagcggctt cttccagggc ttctccgtga cgggaagcgc ctcgcgcaca ccggtggctg
     1801 aagctgccgg agccaagaca caactgacaa gcgtcaccgg cgccatctgc gtggcacttc
     1861 taatcgtcat ttttcccagc ctgctcatga atgtgccgca tacggcacta tcggcggtcg
     1921 ttatttgcgc ctgtctttca ctggttgata ttagcagcgt catcgagcta tacggagtgc
     1981 gcaaagccga gtttgtagcc tgcctggctt gctttctggg tgtggcagta gctggggtta
     2041 tccccggcat attcattgcc gttggtattt cgctcctggt ctttattatc cgcgcctcaa
     2101 gaccctatag cgccgttctt ggcagagcca aagggctcaa gggttaccac gatatcagcc
     2161 ggcaccccga ggccaaacaa ataccgggtc tggtgctctt tcgctgggac gcacctttgt
     2221 ttttcgccaa cgccagactc ttccgggaat gcgccctgga agccattgcc aatgcaccaa
     2281 cggcggtcaa acgaattgtt attgaggccg agcctgtcac cgatatcgac tccacggcag
     2341 cggacgccct gcgggagctt gagcatgact tgaacgcggt ctccattgag ctgggctttg
     2401 cgcaaatgaa ggggccggtg aaagaccagt taaaaaatta cgggctcttc cagaaaatcg
     2461 gcgaagagaa tttcttcccc accctgggcg tggcagttga tgcctacgtg cacacgcacc
     2521 agatagagtg gcacgattgg gacggcaaga agtgctgcaa gcatgc
//
LOCUS       DAOQNZ010000514         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23222_length_2566_cov_3.649542, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000514 DAOQNZ010000000
VERSION     DAOQNZ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23222_length_2566_cov_3.649542"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PKW73_09880"
     CDS             <1..877
                     /locus_tag="PKW73_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73637.1"
                     /translation="SGLERVKVHGAGDYFVLPLQSGFRWMNEAVQPVVARREMSLEVA
                     CNFFLAELLKSTICADLLAENLNQELSALEPPAIGEATLDVDMQDESSTPPMFERAEL
                     PDLAAVCEGELDLPEGQEVFAAELEIITEESATPEALVIADEPEVEAIFQREELPEQE
                     LVEALVEESVEEPVEALVEEVVEEVSSELEVSAAIVERLFQLDPLDEGQSESLPEPAE
                     PGLPTRTVSSDCSIVPAAYGDSDDLQIEADTARPMRYVQLYFPFAEGLVKPTYFEEMP
                     VRRPTKRRGRICLPD"
     gene            complement(899..1093)
                     /locus_tag="PKW73_09885"
     CDS             complement(899..1093)
                     /locus_tag="PKW73_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73638.1"
                     /translation="METLTLPLTLKKLTEIEVEKDGVKNTYWFDQYALDQLGEKLMEL
                     AEAEKESKEEALYKKLNYPL"
     gene            complement(1167..1529)
                     /locus_tag="PKW73_09890"
     CDS             complement(1167..1529)
                     /locus_tag="PKW73_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73639.1"
                     /translation="MEDYREQIIFEDDERRALQNRIDPTRIERYHRMVALLAILIAVV
                     VLRLAVRNSVTDFSIYVPKAFELTILACYPFIFAFVFSSGPPSRLRKFGLFLSVPLGF
                     CMLSYHIHIQVMHGLQIR"
     gene            1672..>2566
                     /locus_tag="PKW73_09895"
     CDS             1672..>2566
                     /locus_tag="PKW73_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73640.1"
                     /translation="MSYIRPRSNTALVFSSVLALFLQTGHSQAAGIGWRAGLEEGDRR
                     MVAKEYPLAEGCFRQALRDVKRDKTSGADDLALCQEKLAAVLYKQDIVDESVPLYLQC
                     LKTLERAHGKESPLLLPRLLELGYIYEFDGNYKQALGVYERAVAIAENAPAGSGGLEL
                     AQCKHRLARALYKSGNFQKAEPLYFSCITLLLGCKTLSTGDLLEACLSDYGDLLEKSY
                     GLGKNLPSEVRGELLRDRLDSLPKKAGVPTSNFSKAVSIKLAEKEDAASAGGTETGAG
                     GDTEDGAHRSVMPPLRGLKTTP"
BASE COUNT          576 a          604 c          751 g          635 t
ORIGIN      
        1 ctcgggcttg gagcgagtga aagtgcacgg tgccggtgac tatttcgttt taccattgca
       61 gagcggcttt cgctggatga acgaggccgt gcaaccggtg gtggcgcggc gggagatgtc
      121 tctggaagta gcctgtaatt tcttcctggc tgaacttctc aagtcgacca tctgtgctga
      181 tcttcttgcc gaaaatttga atcaggaact gagtgccttg gagcctccgg ctatcgggga
      241 agcaacgctt gacgtggata tgcaggacga atcgtcaacg ccccctatgt ttgagcgggc
      301 agagttgccg gatctcgcgg cggtatgcga aggcgagttg gacctgccgg aagggcagga
      361 agtttttgct gccgaattgg aaatcattac ggaagaatca gcgacgcctg aagctcttgt
      421 aattgccgac gaaccggaag tggaggcaat ttttcaaaga gaagaactgc cagagcaaga
      481 attagttgaa gcactggttg aagaatcggt tgaagagccg gtggaagcac tggttgaaga
      541 agtagtggaa gaagtttcct cagaattaga ggtttcggcg gccattgtag agcgcctttt
      601 ccagcttgat ccccttgatg aagggcagtc ggagagctta ccggaaccgg ctgagccagg
      661 acttcccacc cgcactgttt caagcgactg ttctatcgta ccggctgctt acggcgatag
      721 tgatgatctt caaattgaag ctgatactgc gcggcccatg cggtatgtgc agctttactt
      781 tccctttgcc gagggtctgg taaagcccac ctatttcgaa gaaatgccgg tgcgcagacc
      841 cactaagcgc cgggggcgaa tctgcctgcc tgattgaagc aggggctggc tgccgtagtt
      901 aaaggggata gttgagcttt ttatagaggg cttcttcttt actctctttc tctgcctcgg
      961 ccagttccat gagtttttcg cccaattggt cgagtgcgta ttggtcgaac cagtaggtgt
     1021 ttttaacacc gtccttttcc acttctattt ctgtcagttt cttcagtgtc aggggcagag
     1081 tcaaggtttc cataagcttt gatcctcaaa gtggcccaca tggtaataac cgttcggcaa
     1141 agatgaggtt aagaaagtct ggcgagtcag cgaatttgca gaccgtgcat gacttgtata
     1201 tgtatatggt aggacagcat gcaaaagccg agtggaacgc tcaggaagag cccgaatttt
     1261 ctcaagcggc taggaggtcc gctcgaaaat acgaaggcaa agatgaaggg atagcaagcc
     1321 agtatggtta attcgaaagc tttcggcaca tagatgctga agtcggtgac actgtttctc
     1381 acggccaggc gaagaactac caccgctatc agaatggcga ggagggctac catacggtga
     1441 tagcgctcaa ttcttgtggg atcgatccgg ttttgcaggg cccgtctttc atcatcttca
     1501 aaaatgattt gttcgcggta gtcttccatg ggttttccat ttcccctcac ggtgcggcaa
     1561 taacacctta gctagttctt gttagatctg gacttgctcc ggcaagcagc cggttaagat
     1621 tcgggaaaaa tgcagtaaca taaaccaagt tggttttccc gaagagaagc cgtgtcttat
     1681 attcgcccgc ggtcaaatac ggcccttgtt ttcagtagcg tcctggctct gttcttgcag
     1741 accggtcatt cgcaagcggc cggcatcggc tggcgagccg ggctggagga aggcgacagg
     1801 cgcatggttg ccaaagagta ccctctggcg gaaggctgct ttcgccaggc tctacgcgat
     1861 gttaagcgtg acaagaccag tggtgccgat gatctggctc tctgtcaaga gaagctggcg
     1921 gccgttcttt acaagcagga cattgtggat gagtcggtgc ccctctactt gcagtgcttg
     1981 aagacactgg agcgggccca cgggaaggaa agcccgcttt tgctgccgag gcttttggaa
     2041 cttggttata tttatgagtt tgatggtaat tacaaacagg ctctcggtgt ctatgaaaga
     2101 gccgtagcca ttgctgaaaa cgcaccggcc ggtagcggcg ggttggagtt ggctcagtgc
     2161 aagcatcggc tggcgcgtgc cctctacaag agtggaaatt tccagaaggc ggagcctctg
     2221 tatttttcct gcataaccct gcttcttggt tgcaagactc tcagcacggg tgatttgttg
     2281 gaagcctgct tgagcgatta tggcgacctt ctggagaaga gctacggact gggtaagaat
     2341 ctgccttccg aggtgcgcgg cgaacttttg cgcgaccggc tcgacagttt gcctaagaaa
     2401 gccggtgtgc cgacttccaa tttttctaaa gccgtatcga taaagcttgc cgaaaaggaa
     2461 gatgccgctt ctgccggcgg cactgaaaca ggtgctggtg gtgataccga agacggtgcg
     2521 catcgctccg tcatgccgcc cttgcgtgga ctgaagacga cgccgg
//
LOCUS       DAOQNZ010000515         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23301_length_2562_cov_4.008775, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000515 DAOQNZ010000000
VERSION     DAOQNZ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23301_length_2562_cov_4.008775"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKW73_09900"
     CDS             complement(<1..317)
                     /locus_tag="PKW73_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73641.1"
                     /translation="MFHLALAVFCFSLFMAFFVFAFLFLLVWRFFHNRKKRRRLRLVS
                     YQRRLKAIVAELLTDSNELDTASKYVGLDQDAKWVKTYQEALGKLLLSSERLNDCTTF
                     LDAG"
     gene            698..931
                     /locus_tag="PKW73_09905"
     CDS             698..931
                     /locus_tag="PKW73_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73642.1"
                     /translation="MNSPNCSTVKSALTDLREILSRCQINGSAGAPVETARIIANLEA
                     TINCIEEKSLAQGDIPEERGVPIITGMTAPVLG"
     gene            complement(951..2111)
                     /gene="murG"
                     /locus_tag="PKW73_09910"
     CDS             complement(951..2111)
                     /gene="murG"
                     /locus_tag="PKW73_09910"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNA73643.1"
                     /translation="MAEGHRIVLTGGGTGGHIYPALSVAEQLKNDPDVEAILYIGASH
                     HSEERLARENGLDFIGIKVSGMPRRLSPRLLTWPVEMGGAIMEARSILKKFRPTVVLG
                     TGGYASAPPLAAANMLGIPTAVHEPDAHPGLVNRVMGRQAVLISLGMQGAAARMQTKH
                     GRLVFNGNPVRMSFLEERKRDACCAIVGLRSDLKTVLITGGSQGAKALNDAVAGCLSR
                     LLDVEPHLQIVHQCGDKNLNDLKEQLPPGLWESPRYHLRAYFDDLSMAYAICDLAVTR
                     AGAMTVAELAVTGTPAIFVPYPYAAQDHQTHNARFMEAQKAAVVLPQGSLSAEALYEQ
                     IVSLLADDGRLLEMRKHMKEQAKPDAALSLARQLKEISLSYMGATLSAAATR"
     gene            complement(2123..>2562)
                     /locus_tag="PKW73_09915"
     CDS             complement(2123..>2562)
                     /locus_tag="PKW73_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNA73644.1"
                     /translation="QGFGRSLQKLNYLPVQHADFVFAVVAEEFGLIGCSLLIGLFALF
                     GVYGFRTALAARTLFGRFLAIGITSAICTQAIVNMMVTTGLLPVTGITLPFISFGGTS
                     LVITMSMVGVLLSVSRDRGTIHDEDEEEEDDTTPVRPAAATHS"
BASE COUNT          647 a          696 c          697 g          522 t
ORIGIN      
        1 cctgcatcaa ggaaagtagt acagtcattc aggcgctcgg acgagagaag tagcttgccc
       61 agtgcctctt gataggtttt tacccactta gcgtcttgat ccagaccgac atacttactg
      121 gctgtatcaa gctcattact atcggttagc agttcagcta cgatagcctt cagccgacgc
      181 tgataactga ccagacgcaa acgtcgccgt ttcttgcggt tatggaagaa acgccagacc
      241 agaagaaaga ggaaggcgaa gacaaagaac gccatgaaca gcgaaaagca aaatactgct
      301 agagcaaggt gaaacaaacc tgggtcctga aatggcgggt caaagtggga gcggtgagag
      361 gcaacaggca aacctgagat atgccccaat ctgataatac cttgctaaag caaattgctc
      421 agacaaatat tgaatccgcc agagcggacc atgccggaac cgccagatct cttgataact
      481 aaaattaagc tcaaaggtta ttcacgccct gctgcatctt ttcaagcctg tgttacactc
      541 ggaaagttaa tggcaactcc atttgtgccc tccaagtcca ttcagtagat atacagccgt
      601 gggtcaacgc cgaccagacg gatgttaatc aggaatcgct ggcaatccat ccccacgcct
      661 ggcagtcccc gtcgacactg acgagagaga aaaatcaatg aactcgccaa attgttccac
      721 cgttaaatca gccctgacag acctgagaga aatactgagc cgctgccaaa tcaatggaag
      781 tgcaggcgcc ccggtggaaa ccgccagaat catcgctaac ttagaagcaa ccatcaactg
      841 tatcgaagaa aaatctctgg cccagggtga tatcccggaa gagcgcggcg tgcccatcat
      901 caccggcatg actgcaccgg tattgggcta agcttccagc caagtatagt tcagcgcgta
      961 gcagcagccg acagcgtagc acccatatag ctgaggctga tttccttcag ttgcctggcc
     1021 aggctgaggg cggcatcagg ctttgcctgt tccttcatat gtttgcgcat ttccagcaag
     1081 cgcccatcat cagccagcag actcactatt tgctcataga gagcctcagc gctcagtgag
     1141 ccctgaggca agaccacggc tgccttctgc gcttccataa agcgggcatt gtgggtttga
     1201 tggtcctggg cggcataggg ataaggcacg aagatagcag gagtgccggt tacggccagt
     1261 tccgccacag tcatggcacc ggcccgagtc acggccagat cgcaaatggc ataggccatc
     1321 gagagatcgt cgaagtaagc gcgcaggtga tagcgggggc tttcccagag accgggcggc
     1381 aactgttctt tcaggtcgtt gagattctta tcgccgcact gatgcacaat ttgcaggtgc
     1441 ggctctacat ccaataatcg tgacaggcag ccggccacag catcattgag ggctttggcg
     1501 ccctgagaac cgccggttat gagcacagtt ttcaggtcgg agcgcaggcc cacgatggca
     1561 caacaggcat cccgcttgcg ttcttccaaa aagctcatgc gcaccggatt accgttgaaa
     1621 acaaggcgcc cgtgcttggt ctgcatgcga gccgccgcac cttgcatgcc cagggaaatg
     1681 agcaccgctt gccgccccat gacacggttt accagcccgg gatgcgcatc cggttcgtgc
     1741 acggcagtgg gtattcccag catattggcc gccgccaggg gtggtgccga ggcgtagccg
     1801 cctgtaccaa gcacaacggt gggacggaat tttttcaaaa tgctgcgggc ttccataatg
     1861 gcgccgccca tttccacggg ccaggtaagc aagcgaggag aaagccggcg cggcatgccg
     1921 gaaactttga tgcctataaa atcaagtccg ttttcccgag ccaatctttc ctcggagtga
     1981 tgagaagcac ctatataaag gattgcttcc acatccggat cgttcttcaa ttgttcggcc
     2041 acggagagcg ccgggtatat atggccgcct gtgcctccgc cggtgagaac aattctgtga
     2101 ccttcagcca ttattttctc gctcaggaat gagttgccgc cgccggtctg accggagtag
     2161 tgtcgtcttc ctcttcctca tcttcatcgt ggattgtgcc gcgatcacga gagacggaga
     2221 gaagtacgcc caccatggac atggttataa ccagtgatgt gccgccaaag ctgatgaagg
     2281 gcagggtgat accggtgacg ggaagaagtc cggtggtgac catcatgttg actatggctt
     2341 gagtgcaaat ggcgctcgtg attcctatag ccaggaaacg cccgaagaga gtgcgggcgg
     2401 ccagtgccgt gcggaagcca taaacgccaa agagagcgaa gagcccgatt aatagagagc
     2461 agccaatcaa gccgaattct tccgccacga cggcgaaaac gaagtcggcg tgctgcacag
     2521 gcagatagtt gagcttttgc aaggaacgtc cgaaaccctg tc
//
LOCUS       DAOQNZ010000516         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23307_length_2562_cov_3.516554, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000516 DAOQNZ010000000
VERSION     DAOQNZ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23307_length_2562_cov_3.516554"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2084
                     /locus_tag="PKW73_09920"
     CDS             <1..2084
                     /locus_tag="PKW73_09920"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNA73645.1"
                     /translation="GYSMGTVCGASRRRYHCVMASALATPVARNIVLSRVEELVTVNG
                     TQYELATNAWASGVISPTGYKLLESFTNLPCPTWVYDLNGHYLIKRLVMPHGRNEVYF
                     GYTFLPFSNMPYSPDLPEAGAGADTPDVKLTTRFLVGFRNFHGQVKGSSDDRYPQFVS
                     PNQSMVILSETGKRLCLAWKEGQYETQKQWWWDYKWVEEAARGEPDSEDLFLVGTVSM
                     KMEPGKEYSLGASFENAISMPDTNKVVEESIARQSFLVRRASLPRSIKTDMLVLACDQ
                     FIVPAFEEQQGNWGRPTGVSLLPSESIPEYLSVIEGYPWFNDSGRAALTSLPGLCLAT
                     RRFAEAKAILKNFSRRRQGALIANRTLDPPRPVTSQSRPALEFTSLDTSLWFAWSCHH
                     YYRITRDKELIKELFETLLDIYGSLQNGTTPGVTIDKRDGLLHTDLPGTEFSWMDARV
                     AEIPITPRPGKAVELNALWFNFLSTILAFAQDQELNLSLPSSLEKSLKEAIETVQASM
                     QRFWNEENSCLYDVIDMPPGVADKGPKKDSSVRPNQLLAVALPFRALSREQEKSILHA
                     VESQLVTPMGLRTLSPEDGQYQASYGCGFDHADQYHRDLSYHQGTVWPWLLGIYMEAL
                     LNVYGPLPETVSRIRILLSPMLEHLTQEALLGGISEIFDGNRPHLPRGCPNQAQAVAE
                     CMRWQNWLLKQ"
     gene            2363..>2562
                     /locus_tag="PKW73_09925"
     CDS             2363..>2562
                     /locus_tag="PKW73_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73646.1"
                     /translation="MWEAPTTQNVADLLEKAKKRPGSSVDLPYSVPGCNSLYQLGVVV
                     KENQDPEWTFKTGTLTGLSVKWT"
BASE COUNT          630 a          758 c          644 g          530 t
ORIGIN      
        1 gtggttactc catgggaacc gtctgcggcg ccagtcgtag acgttaccac tgtgtcatgg
       61 cttccgccct ggccactccg gtagctcgca atattgttct ttccagggtg gaagaactgg
      121 tcaccgtcaa tggcacccaa tatgagctag ccactaacgc ctgggcttcc ggcgtcatct
      181 cccccaccgg ctacaagctt ttggaaagtt tcaccaacct gccctgcccg acctgggttt
      241 acgatttaaa cggacactac ctgatcaagc gactggtcat gccccatggc agaaacgagg
      301 tctatttcgg ctataccttc ttaccgtttt cgaatatgcc atattcaccg gacttgccgg
      361 aagcgggagc cggcgccgat accccggatg taaaactgac cactagattt ctagtgggtt
      421 tccgaaattt tcatggtcag gtgaaaggtt cctccgacga ccgctatcca caattcgttt
      481 cacccaacca gtccatggtc atcctttccg aaaccggcaa acggctttgc ctggcctgga
      541 aggaaggaca gtacgaaacc cagaaacaat ggtggtggga ttacaaatgg gtggaagaag
      601 cagcaagagg agagccggat agcgaagatc tctttctggt gggcacggtc tccatgaaga
      661 tggaaccagg gaaggaatac tctctgggag ccagtttcga aaatgccatc agcatgcccg
      721 acacaaacaa agtcgtggaa gaatccattg ccagacagtc cttcctggtg cgccgggcca
      781 gtctgccccg ctcaatcaaa accgacatgc tcgttctggc ttgcgaccag tttatcgtgc
      841 cggccttcga agaacagcaa ggcaactggg gcagaccaac gggtgtaagc cttttgccct
      901 ccgagagcat acctgagtac ctttcagtga tcgagggtta tccctggttc aatgactccg
      961 gcagagcggc ccttacctct ttacccggtc tctgcctggc cacacggcgc tttgccgaag
     1021 caaaagcgat actgaaaaac ttcagccgcc gccgccaggg cgccctcatt gccaatcgca
     1081 ctctcgatcc gccccgaccg gtcaccagcc agtcgcgccc cgcgctggaa ttcacctccc
     1141 tggataccag cctctggttc gcctggtcct gccatcacta ctatcgcatc acccgcgata
     1201 aagagctgat caaagagctg ttcgaaacct tgttggatat ctacggcagc cttcagaatg
     1261 gtactacccc cggtgtgact atcgacaagc gggacggtct tttgcatacc gacttgccag
     1321 gaacagagtt tagttggatg gatgcccgag tagccgagat tcccattaca ccgcgtccag
     1381 gcaaggcggt agaactcaat gccctctggt tcaacttcct gagtaccata ctggcattcg
     1441 cccaggatca agagctgaac ctgagcctgc caagcagcct ggaaaagagc ctcaaagaag
     1501 ccatagagac cgttcaagca tccatgcagc gtttctggaa cgaagaaaat tcctgcctct
     1561 atgacgtaat cgacatgcca ccgggtgtgg ctgataaagg accaaaaaaa gacagctccg
     1621 tgcgccccaa tcaattgctg gcggtggcgc tgcctttccg cgccctgagc cgtgagcagg
     1681 aaaagtccat cctccatgcc gtggaaagcc aactggttac gcccatggga ttacgcaccc
     1741 tctctcccga ggatggacag tatcaagcca gctacggctg cggcttcgac cacgccgatc
     1801 aatatcaccg cgacctttcc taccatcaag gtactgtctg gccctggctc ttgggcatct
     1861 acatggaagc cctgttgaac gtttatggtc ccctgccgga aacggtgagc cgtatccgca
     1921 ttctgctttc acccatgctg gagcatttga cgcaggaagc tctactgggc ggcatatccg
     1981 aaattttcga cggcaatcgc ccccacttgc ccaggggctg ccccaatcag gcccaggcag
     2041 tggccgaatg tatgcgctgg caaaactggc tgctcaagca atagccgctg cctttcccca
     2101 tagcttcaat cagggcggcg ccatgaacgc caccttaatt caccgggacc aaaccggaaa
     2161 aagagcatgt cttaaagatg acgtatgggg gtatgaaaaa tgcaacagga actgcgctca
     2221 atttcctgcc acgaaattaa gtgaaataga aaaggctatc cctgggctga tgttcaaatc
     2281 caacctaaac cgcggccaat cgcaaaacca acaatctcaa aaggaaacaa agccggatac
     2341 tggacctttg cggctaccgc aattgtggga agcgccgacc acccagaacg tagcggacct
     2401 tctggaaaag gcaaagaaaa gaccgggttc ctctgtggac ctgccctact ccgtgcctgg
     2461 ctgcaacagc ctataccagt tgggcgtagt cgtaaaagag aatcaagacc ctgaatggac
     2521 ctttaaaacc ggcaccctga ccgggctgtc cgtcaagtgg ac
//
LOCUS       DAOQNZ010000517         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23316_length_2561_cov_4.375100, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000517 DAOQNZ010000000
VERSION     DAOQNZ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23316_length_2561_cov_4.375100"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="PKW73_09930"
     CDS             complement(<1..1857)
                     /locus_tag="PKW73_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73647.1"
                     /translation="MSTPKNSLWENFLTVIFFIPVTTYQLLRESGEWLWKRISRANGW
                     ERSLIVAVVGLPISGASAWQVANLLAFHYGLGGLSWFFCFIVSAVLIWSHAWSSLYYF
                     ALRHVWNLCEKAFKQWDRFCRNGLHPMLRALTDWTRQAPGANKLWLAVEGEDGRSGTA
                     VRVLEVILGVSIFGGAAYTGYLTYGAVTAAALVDSGTLMMILGLTAAATVFGSIMMFF
                     ISMAEGKAPAVVAAYSALLAGATAYAATQFPLVSQTPVSNSAGIALGVFDFFFNVTYV
                     LPLAISLMQSGTIKRLVERWFDLIKDSYAEEENKDFQRFYQHALNLTLAPVLGFTIYI
                     MATASALPVWSAALLAAATAIYSYCEAFRKRLNSYRCNLEMGTTIVFLAGAAAFTFMP
                     ATNAAISVAWAIGSMVFTYLFAYPAFYLTTRMIATRVNVGTVNCGTLGLTLEKLHGSL
                     SASVTAMFEALRTKRREAFDDNSDFSALFGHVLNLVIVATAVYSGTPVAMDYLTSSFW
                     LNAAIAFFVATNGFVLLGKLFSSYSAHTWAAVLGLSSAAFTGYYSYQVSDSVVTAVVL
                     AAAVADIAVYVVAPLSYLAVRNVTAGVTPALAKFARDSFDAIWSIYVRIWAAI"
     gene            complement(2031..>2561)
                     /locus_tag="PKW73_09935"
     CDS             complement(2031..>2561)
                     /locus_tag="PKW73_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73648.1"
                     /translation="TQGQWSKQFGGINDANQAQWNQQTGKFNDANQGQWNQQHGWVNN
                     ADQAQWNQQTGKFNDATQGQFNKQFGGFNDATQGQWNQQHGWVNNADQAQWNQQTGKF
                     NDASQGQFNKQFGGINNAEQSQWNQQNGRFNSADQTQLNFQRGWNNTYEGSQFSSQTG
                     RYNDSTQFQQSIQVSP"
BASE COUNT          575 a          632 c          757 g          597 t
ORIGIN      
        1 gattgctgcc cagattctga cgtagatgga ccagatggcg tcgaagctat ctctggcaaa
       61 tttggccagg gcaggagtga ctccagcggt gacattgcgc acggccaggt aagagagcgg
      121 tgctaccaca tagacggcga tgtctgccac tgctgcagct agaactacgg ccgtgactac
      181 gctgtcgctg acctgataag agtagtagcc tgtgaaggca gcggaactca gacccagaac
      241 agcggcccag gtgtgggcgc tatagcttga gaagagtttg cccaacagca cgaaaccgtt
      301 agtggctacg aagaaggcga tagctgcgtt taaccagaag gagctggtca aatagtccat
      361 ggctacggga gtgcccgagt atacggcggt ggctacgata accaggttaa gcacgtgacc
      421 gaaaagcgcg gagaagtcgg agttgtcgtc gaaagcttct cttcttttgg tgcgcaatgc
      481 ttcaaacatg gcggttactg aggctgaaag actgccgtgc agcttttcca gggtcaagcc
      541 cagggtgccg caattgacgg tgcctacgtt gacgcgagtg gcaatcatac gtgtggtcag
      601 gtagaaagcc ggataggcga acaggtaagt gaaaaccatg gagccgattg cccaggcgac
      661 gcttatggcg gcattggtag caggcatgaa ggtgaatgca gcagcgccgg ccagaaagac
      721 gatggtggtg cccatttcca gattgcagcg gtaggaattg agacgtttgc ggaaagcttc
      781 gcaatagctg taaatagcag ttgcggcggc aagcaaggcg gctgaccaga ccggcaaggc
      841 actggctgtg gccattatgt agatggtaaa gccaagaacc ggagccaatg tcaggttaag
      901 tgcatgctgg tagaaacgtt ggaagtcttt gttctcttct tccgcgtagg agtctttgat
      961 caggtcgaac cagcgctcta caaggcgttt gatggtgccg gactgcatca ggcttatggc
     1021 cagcggcagt acataggtga cgttgaaaaa gaaatcaaag acgcccagtg cgatgcctgc
     1081 ggaattgctg actggagttt ggctgaccag gggaaactgc gtggccgcgt aagcggtggc
     1141 gcctgcaaga agggcactgt aagcggcaac gactgccggt gctttaccct ccgccatact
     1201 gataaagaac atcatgattg agccgaagac tgtggcggcc gccgtcagtc ccaggatcat
     1261 catgagggtg ccggaatcaa ccagagccgc agccgttact gctccatagg tcagatagcc
     1321 tgtgtaggcg gcgccgccga atatggaaac acccagaatt acttccagta cgcgcactgc
     1381 tgtgccgctt cgcccgtctt caccctcgac agccaaccag agcttattgg ctcccggtgc
     1441 ctgtcttgtc cagtcggtta aggctcgcaa catagggtgc aagccgtttc gacagaaacg
     1501 gtcccactgt ttgaaggcct tctcgcagag attccagaca tgcctcagtg cgaaatagta
     1561 gagtgatgac caggcatggg accagatcaa taccgcgcta acgataaagc agaagaacca
     1621 actgagacct cccagaccat agtggaaagc gagaagattg gctacttgcc aggcgctggc
     1681 gccgctgatt ggcagaccca cgacggccac tatcaaggaa cgttcccagc cgtttgctct
     1741 gctgatgcgt ttccaaagcc attccccgct ttctctcaat agctggtaag tggtaacggg
     1801 aatgaagaag atgacagtga ggaagttttc ccagagggag tttttcggtg tagacatact
     1861 ttagtcctgt attcgaatta aagttgaaaa aggctcgacc cgacagaatt ctgccgggtc
     1921 gatgttgtta gtcttgtccc tgactatggt gggagcctga ggaagcgcat ccattgcagg
     1981 acatcacttt caggctccgg gttagtgcac tgaatccgct tcgttcagtc ttacggactt
     2041 acttgaatgc tttgttggaa ctgagtgctg tcgttatagc ggccggtttg tgaggaaaac
     2101 tggctgcctt cataggtgtt gttccagcca cgttggaagt tcaactgggt ctgatcggcg
     2161 ctgttaaagc ggccgttttg ttgattccac tggctttgtt cggcgttgtt gataccaccg
     2221 aactgcttgt tgaactgacc ctggctggcg tcgttgaatt taccggtttg ctggttccac
     2281 tgcgcttgat cggcgttgtt tacccagccg tgttgctggt tccactgccc ctgggtggcg
     2341 tcgttgaaac cgccgaactg cttgttgaac tgaccctgag tagcgtcgtt gaacttaccg
     2401 gtttgctggt tccactgcgc ctgatcagcg ttgtttaccc agccgtgttg ctggttccac
     2461 tgaccctgat tggcgtcgtt gaatttaccg gtttgttggt tccactgcgc ttgattggcg
     2521 tcgttgatgc cgccgaactg cttgctccat tgaccctggg t
//
LOCUS       DAOQNZ010000518         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23359_length_2559_cov_3.387380, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000518 DAOQNZ010000000
VERSION     DAOQNZ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23359_length_2559_cov_3.387380"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..628
                     /locus_tag="PKW73_09940"
     CDS             254..628
                     /locus_tag="PKW73_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73649.1"
                     /translation="MASFSAARAGSFLAPVDGHPSAVTEAQSKLQNAKNAFSGIDVEN
                     IKTEILITNIDTQSTVARLSPLTDPADVSVNTYQIQVSARCSASPLVPIPSPIPIAGV
                     SAPLVFDMTARQYCENPQGLTL"
     gene            679..2463
                     /locus_tag="PKW73_09945"
     CDS             679..2463
                     /locus_tag="PKW73_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA73650.1"
                     /translation="MSHLNKPLSRGRSRGQGILSLLMLFALVLFPLIAVLVFEFSRLY
                     LAKQELQNASDAAALTGAATLASSDNTNPTQAHLDAIESALKIFKSNTSLGVPLSNSV
                     VVASPSLLECASGEAKLFFEFLNPVTMLPEPITSANGKVVKIRSCNGSDLAFGKFLGI
                     KSWGVTAVSTSAVPKLDIVICFDVSGSMDDQTPVTFSKRVWDDTLGGGKIVYSSVNGA
                     NGIMQGRIFDILKPAITGTSLNGTFPQLLTESYWNAKTYFSEYLASYYGVSGLRSGGV
                     YPEAGAPPGNCPPGTAFTFDGFKTFTDVVVNIDGNTTFGGFSYDGYAFPDLATLIEAS
                     RGNLENSSVFSSSRANTAVSVSPKPGYQKAYFEAAQKQLKPMADAKEAVLTMTQILNT
                     DADSHFGFVAFDADVGVDSASTEQWHNLDDAAPYGVLQGFPLPRVTISKNPGSTQFSQ
                     VNTSIKSCVPLGATNIGASVHAAVQDLKSNSRTGSVKAIILFTDGEPTVPAGPLSSDP
                     EANARLAAVEARDAGIAVYTIGLAQNPMIIADQEKILNDTNSDPTTGGMAAIAGHGGT
                     FNLVTDSSQLRTTFAKIARRLVKLVSNK"
BASE COUNT          543 a          693 c          663 g          660 t
ORIGIN      
        1 cgctgattgt ttaattgcgg cactatttta agcgggtagt aggaagatga aattcaaggc
       61 aaaggcatta actgagctaa gtgttggctc tctcccgaga gtcgggggct gtcggcggcg
      121 ccgacccggc cgcggctcgc ttctggctga actgccgatt gtcttgtgga tcttgtttgt
      181 tgtcattgcc tttccgctgc ttaatttgac tgcggccttc ttgcgctgtt ccttccttta
      241 cgcaggcatt cacatggcca gcttttctgc ggcccgtgcc ggtagtttcc ttgctccggt
      301 ggacggtcat ccctctgccg tgactgaagc gcagagcaaa ctgcaaaatg ctaagaatgc
      361 cttctcgggt attgatgtcg aaaacatcaa gactgagata ctgatcacca atatagatac
      421 gcagtccact gttgctcgtc tttctccttt gactgatcct gccgatgtca gtgtcaacac
      481 gtaccagata caggtatctg cccgctgctc tgccagtcct cttgtgccga ttcccagtcc
      541 cataccgatt gccggtgtgt ccgctccgct ggtgttcgat atgaccgcca ggcaatactg
      601 cgaaaacccg cagggtctga ccctgtagtg agcaccgctt ccctttagtt tcttagagta
      661 ttttgcaggt ttccaaacat gtctcattta aataaaccac taagcagagg tcgttccaga
      721 gggcagggca tcctctcttt gctcatgctc tttgctctgg tgctttttcc tctcattgcg
      781 gtgctggtct tcgaattttc caggctctat ctggctaagc aggaactaca gaacgcctct
      841 gatgctgcgg ctcttaccgg agccgccaca cttgccagct ccgataacac caacccgacg
      901 caagcgcacc tcgatgccat cgagagtgcc ctcaagatat tcaagtcgaa tacttccctg
      961 ggcgtgcctt tgagcaactc cgtcgtggtt gcctctcctt ctcttttgga gtgtgcaagc
     1021 ggcgaagcca aactcttttt cgaattcctc aatccggtga ccatgctgcc ggagcccatt
     1081 acgagtgcca atggcaaggt ggttaagata cgctcctgca acggcagcga cttggcgttc
     1141 ggtaagtttc tcggaattaa atcatggggc gtgacggccg tgtctaccag tgccgttccc
     1201 aagctggaca tagtcatatg ctttgacgtc tccggttcca tggacgatca gactcctgtt
     1261 actttcagca aacgcgtctg ggacgacacc cttgggggcg gcaaaattgt ctattccagc
     1321 gttaatggtg caaatggaat catgcagggg cgaatcttcg acattctcaa gcctgccata
     1381 accggaacta gcttgaatgg aacctttccc cagcttttaa ctgagtctta ttggaatgcc
     1441 aaaacctatt tctccgagta tctggcaagc tattacggtg taagcggatt gcgttccggc
     1501 ggcgtctatc ccgaggccgg tgctccgcct ggaaactgtc cgcctggtac ggccttcact
     1561 tttgatggct tcaagacctt cactgatgtg gtggtaaaca tagacggcaa tactaccttt
     1621 ggtggcttta gctatgatgg atatgccttc ccagatctgg ccacgctgat tgaagcttct
     1681 cgaggcaacc tggagaatag ctcggtcttc agcagcagta gagccaacac agccgtgtcg
     1741 gtgtctccca agccaggcta ccagaaggca tattttgaag cggcccagaa gcaattgaag
     1801 cccatggctg atgctaaaga ggcagtattg accatgactc aaatactcaa taccgatgca
     1861 gatagtcatt tcggtttcgt tgcatttgat gctgatgttg gcgtcgatag tgcgagcacg
     1921 gagcaatggc acaatctgga tgacgctgct ccttatggcg ttttgcaggg attccctctg
     1981 ccgcgggtaa ctatcagcaa gaatccaggc tccacgcagt tcagccaggt gaatacttcc
     2041 atcaagagtt gtgtgcccct cggtgccact aatattggtg cgtctgtgca tgctgcggtg
     2101 caagacctga agagtaatag tcgcacaggc agtgttaagg caataattct tttcaccgat
     2161 ggcgagccga cggttccggc cggtcctttg agttccgacc cggaggcaaa tgccagactc
     2221 gctgccgtag aggcgcgcga cgccggaatc gccgtatata ccatcggctt ggcccagaat
     2281 ccgatgatta ttgccgacca ggaaaagatt ctcaacgaca ccaactccga ccctaccacg
     2341 ggaggcatgg ccgccatcgc cggtcatggc ggtaccttca atctggttac cgatagttct
     2401 cagttacgca ccacctttgc caagattgcc aggcgccttg tgaagctggt gtcgaataag
     2461 tagggggatt ctaatcatgt catcgcaagc agggttgatt gtaatttccg gcaggcgctc
     2521 ttgccggggc tcactgtcgg tggaactggc cagcggcag
//
LOCUS       DAOQNZ010000519         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23397_length_2557_cov_3.063549, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000519 DAOQNZ010000000
VERSION     DAOQNZ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23397_length_2557_cov_3.063549"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="PKW73_09950"
     CDS             <1..1594
                     /locus_tag="PKW73_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNA73651.1"
                     /translation="EPNQLNQLLLPSFVVEDKARAKKEGRLLAQGLNASPGAAIGKIV
                     FNPDESEKRATSGEKIILVRIETCPDDIHGLVPAQGVVTSRGGMTSHAAVVARGMGKP
                     CVAGCEVLKIDLEKEEMVVSGKTFKKGDVISIDGTTGEIFEGAIETSDPKFSEDFETL
                     LGWADSVRKLLVRTNADTPEDARVARGFGAQGIGLCRTEHMFMNHDRLPVVQQMIMSV
                     NDAERQTALDKLLPMQRQDFDGIFRAMSGLPVTIRLLDPPLHEFLPKEEHLIEEVATM
                     KAKGQSGAELREKEVLLRKVRELKESNPMMGLRGCRLGLVYPQINKMQVQAIFEAAIA
                     VKRDGIEVLPEIMIPLVGHVNELDFARQQLESVAREVMDREKVEISYKFGTMIEIPRA
                     ALTADAIARHAEFFSFGTNDLTQMTFGYSRDDAEGKFLQRYLEGVEFGGSKHKVLLNN
                     PFEVLDREGVGQLMKIAVELGLKTRPDLKLGICGEHGGEPSSIAFAHELGLSYVSCSP
                     FRLPVARLAAAQANLKMHERDK"
     gene            1700..>2557
                     /locus_tag="PKW73_09955"
     CDS             1700..>2557
                     /locus_tag="PKW73_09955"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73652.1"
                     /translation="MSIDENVDALLRLLPEMEQSLGLEHPDLADYLISLGLLLYHDSR
                     FDEAENLFARALAMRERLLAVDHPDVAAALNHLGLVYLAQGRYAEAEPLFKRALSVQE
                     KTLGPEHLSIASALSNMGDLYITLNRYGEAEEVIKRAIKIRERYLGLEDSGLVEVLNS
                     LANIYYEQGRNSDAEPLFRRVLKIEERIHGHDHPNVANALNNLAALFTDREQFDQAEP
                     LHRRALAIREKVLSPDHPKVAESLNNLGWLYHQHGNHARAESLYKRAIEIWERALGPD
                     HSFVAACLEN"
BASE COUNT          558 a          680 c          756 g          563 t
ORIGIN      
        1 agagccgaac cagctcaatc aattgctttt gccctccttt gtggtggaag acaaggcgcg
       61 ggccaagaaa gaaggacggc ttctggctca ggggttgaac gcttctcccg gagcggctat
      121 cggcaagatc gtctttaatc cggacgagtc ggaaaaacgt gctacttccg gcgagaagat
      181 aattcttgtg cggattgaaa cctgccccga cgatattcac ggtctcgtac cggcccaggg
      241 ggttgtcacc agtcgcggtg gcatgaccag ccatgccgcc gtggtggcca gaggcatggg
      301 caaaccctgc gtggcaggct gtgaagtgct caagattgat cttgagaagg aagaaatggt
      361 ggtgagcggt aagaccttca agaaaggcga cgttatttcc atcgacggca ccaccggcga
      421 aatcttcgaa ggtgccatcg aaacttccga tcccaagttc tcggaagatt tcgagacctt
      481 acttggatgg gctgactccg tgcgcaaact tttggtgcgt accaatgccg ataccccgga
      541 agacgcccgg gtggctcgcg gcttcggggc ccagggcatt ggtctttgcc gtaccgagca
      601 tatgttcatg aatcatgacc gcctgcctgt tgtgcagcag atgattatgt cggtgaatga
      661 cgccgagcgc cagactgctc tcgataagct tctgcccatg cagcgtcaag actttgacgg
      721 tatcttccgg gccatgagcg ggctgcctgt cacgattcgc cttctggatc cgcccctgca
      781 cgagtttctg cccaaagaag agcacctcat tgaagaagtg gcgaccatga aggccaaagg
      841 gcagagtggt gccgaactgc gggagaagga agtgcttctg cgtaaggtgc gggagctgaa
      901 agagtccaat cccatgatgg gactgcgggg ctgccgcctg ggtctggttt atccccagat
      961 caataagatg caggtgcagg ccattttcga ggcggctatt gccgtcaagc gcgacggcat
     1021 cgaggtcttg cctgagatca tgattcctct tgtgggtcat gtgaacgagc ttgattttgc
     1081 ccgtcagcaa ctggaaagcg tcgcccggga agtgatggac cgggagaagg tggaaatctc
     1141 ctacaagttc ggcaccatga tcgagattcc ccgggcggcc ctcaccgccg acgctatcgc
     1201 caggcatgcc gagttcttca gcttcggcac caatgacctg acccagatga ctttcggtta
     1261 ttcccgggat gatgccgaag gcaaattcct gcagcgctat ctggaaggtg ttgaatttgg
     1321 cggctccaag cacaaagtgc tgctcaataa tcccttcgag gtactggacc gggaaggggt
     1381 gggtcaactg atgaagatcg ccgtggaact tgggctcaag actcgtcccg acctgaaact
     1441 cggcatttgc ggcgagcatg gcggggaacc cagttccatt gcctttgccc atgaactggg
     1501 gctctcttat gtgagctgct cgcccttccg tctgccggta gcgcggctgg cggctgccca
     1561 ggccaatctg aagatgcatg agcgcgacaa gtaggcaaat tgttagttaa tgacaattct
     1621 cttgtacata ggttagacaa gagcctcagc ctggtaatta agtattagct tggtctttta
     1681 gggaaaggtc actggctaga tgtccattga cgaaaatgtg gatgcactat tgcgattgct
     1741 gccggagatg gagcagtcgc tggggctgga acatcccgat ctggctgatt acttgatctc
     1801 gctggggctt ttgctctatc acgacagtcg cttcgacgag gctgagaacc tcttcgcccg
     1861 ggccctggcc atgcgagagc gccttctggc cgtggaccat cccgatgtgg ccgccgccct
     1921 caatcatctg ggtctggtct acctggccca ggggcgctat gccgaagccg aaccgctctt
     1981 caaaagagcc ctttccgtgc aggagaaaac cctcgggccg gagcacttat ccattgcctc
     2041 ggccctttcc aatatgggcg atctctacat aaccttgaac cgctatggtg aagcagaaga
     2101 ggtcataaag cgagccatta agataaggga gcgctacctc ggtctggagg atagcggtct
     2161 ggtggaggtg ctcaatagcc tggctaacat ttattatgag cagggccgca acagcgatgc
     2221 cgagccgttg tttcgccggg tgctcaagat agaggagcgc attcacgggc acgaccatcc
     2281 caatgtggct aatgctttga ataacctggc cgctctcttt accgacaggg aacagtttga
     2341 ccaggctgag cctttgcacc ggcgggccct ggctattaga gagaaagttt tgagtcccga
     2401 tcatcccaag gtggcggaga gcctgaacaa cttgggttgg ctctaccatc agcacggcaa
     2461 ccatgcccgg gctgaaagtc tctacaaacg agccattgaa atctgggagc gggcccttgg
     2521 acccgatcac tccttcgtgg cggcctgtct ggaaaat
//
LOCUS       DAOQNZ010000520         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23421_length_2556_cov_3.114354, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000520 DAOQNZ010000000
VERSION     DAOQNZ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23421_length_2556_cov_3.114354"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..718
                     /gene="purE"
                     /locus_tag="PKW73_09960"
     CDS             230..718
                     /gene="purE"
                     /locus_tag="PKW73_09960"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNA73653.1"
                     /translation="MGSQSDYKVMVHAREILVRAEVPHETLVVSAHRTPERMYEYGQS
                     AQGRGLKVIIAGAGGAAHLPGMLSSLTALPVLGVPVPGSNLSGLDSLLSIVQMPGGVP
                     TATFAVGKAGAVNAALFAIKILALESGNEKLAHFLTMERQRRADEAIASSDQLLDFDG
                     HL"
     gene            732..1883
                     /locus_tag="PKW73_09965"
     CDS             732..1883
                     /locus_tag="PKW73_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNA73654.1"
                     /translation="MKVVGILGGGQLGLFLSQSLARLGAATAVFDPDLEAPTHRHTAA
                     AFPYPFTDQEKLRQFEELSDVITYEFEHLPVSVLSTMAEHESLFRPSLKVLAVAQDRL
                     MEKCFLRDKHFPVVDFLAIEKKEDLAAFEQFGLPCLVKTVRGGYDGKGQKRLKSEAER
                     QSFLPELLARLERGETMVAEKLIDLSLEMSCIVARQKGRCVTMPVFENVHKDSILDTT
                     VFPSSLSKELDAGVRKLACDIAAALDVEGLLTVEFFLSGQDIFVNELAPRPHNSGHLS
                     MQAFNLSQFDLLSRILLDIPIADCEPISEQAFAMANILGDCYYRDGDRSTLNMKALEQ
                     APGFVELLLYGKQEPRKARKMGHLVCRGATPAEALNRALALRKALSSPA"
     gene            1895..>2556
                     /locus_tag="PKW73_09970"
     CDS             1895..>2556
                     /locus_tag="PKW73_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73655.1"
                     /translation="MSRTKQITAALCFTLALLVSPGAALRAQDKNEAQPAEPEEAFPA
                     FQMDSLQQFLSPALLMKGKVAFDPGLKVYCFTYKSCRVSLLLLDSKKLTLKPALASSG
                     TEPTEMVAAGSGALAATNGGYFNLSDGKSTSFVVLDGRLVADPRENKALTGNPRLTAF
                     LPKIYERSELRVYQDKLGKISYAIAAHSDAVPAALTLKHSLQGGPRLLPQLTAEAEAF
                     LRT"
BASE COUNT          543 a          684 c          710 g          619 t
ORIGIN      
        1 ttcttcccca atgtggacag catcgaagaa gccgccgccc atggtatcaa ggtaattatc
       61 cagccgggcg gtagcatcaa agataaagac gtcattgcta aagctcagga actgggcgtg
      121 accatgcttc tcaccggtca gcgctgtttc cgtcactgag gtgcccatgt ctgaaagaac
      181 agatgtcgcc aataagcctc cggccagaat tcctcttgta ggtctcgtta tggggagcca
      241 gtcggactac aaggtgatgg tgcatgccag ggaaattctg gtgcgggcgg aagtgccaca
      301 tgagaccctg gtggtcagtg cccatcgcac ccctgagcgt atgtacgagt acggtcagag
      361 cgctcagggg cgcggtctca aggtgattat tgccggtgcc ggcggggccg cccacctgcc
      421 cggcatgctc tccagtttga cggccctgcc tgtgctcggc gtgccggtgc ccggcagcaa
      481 tttgtccggg ctcgacagcc ttctttctat tgtgcagatg cccggcggcg ttcctaccgc
      541 cacctttgcc gttggtaagg ctggtgccgt caacgccgct ctctttgcca taaagattct
      601 cgcccttgag agcggcaacg agaaactggc ccattttctg accatggaaa gacaacgccg
      661 ggctgatgaa gcaattgcct ccagcgacca attgctcgat tttgatgggc atctctaaag
      721 gagaccttag cttgaaggtt gttggaattt taggcggtgg gcaattgggg ctcttcctga
      781 gccagagcct tgcccgcctc ggcgctgcca ctgccgtgtt tgatccggat ctggaggcgc
      841 cgacccatcg gcatacggct gccgcttttc cctatccttt taccgaccag gaaaagctca
      901 ggcagtttga agaactgtcc gatgtcatca cctatgaatt cgaacacctg cctgtcagtg
      961 tgctctcaac catggcagag catgagagcc tcttcagacc ttctctcaag gttctggcag
     1021 tggctcaaga tcgcctcatg gagaaatgct ttttgcgtga caagcatttc ccggtggtgg
     1081 attttctagc catcgaaaag aaagaagacc ttgctgcttt cgagcaattt gggctgccct
     1141 gtctggtcaa gacggtacgg ggcggctacg atggcaaagg gcaaaaaagg ctcaagagcg
     1201 aggcggaaag gcagtccttc ttgccggaac tgcttgcgcg cctcgaacgg ggcgagacaa
     1261 tggtggcgga aaaactcata gacctcagtc tggagatgag ttgcattgtg gccaggcaga
     1321 aaggccgctg cgttaccatg cccgtctttg aaaatgtcca caaagattcg attctggata
     1381 ccacggtctt tccctccagt ctctctaaag agttggatgc cggggtgaga aaattggcct
     1441 gcgatatcgc agcagcactt gatgtggaag ggctcttgac cgttgaattt ttcttgagcg
     1501 ggcaggacat ttttgtaaac gagctggcgc ctcgccctca taattccggg catctttcca
     1561 tgcaggcttt caatttaagc cagttcgact tgctttctcg aatcttgctc gacataccca
     1621 ttgccgattg cgagcccatt tccgaacagg cttttgccat ggccaatata ctgggcgatt
     1681 gctattaccg ggacggtgat cgttccaccc tcaatatgaa ggcccttgag caggctcccg
     1741 gttttgtgga attgcttttg tacggcaagc aggaacctag aaaagctcgt aaaatgggcc
     1801 atctggtttg ccggggggca acgccggcgg aagctctaaa ccgggctctg gctcttcgaa
     1861 aggcgttgtc tagcccagct taagggggaa acaattgagt cggactaagc agataacggc
     1921 ggcgctgtgt tttacactcg ccctactggt ctcacccgga gccgctctcc gggcgcagga
     1981 caagaatgag gcgcagccgg cggagcctga ggaagccttt cctgcttttc agatggactc
     2041 actgcagcag ttcctttctc ccgccctttt gatgaaaggc aaagtcgctt ttgatccggg
     2101 tttgaaagtc tattgtttta cttataaaag ttgtcgggtc agtttgctct tattggatag
     2161 taagaaactg accttgaaac cggcactggc ttcctccggt accgagccta ccgagatggt
     2221 ggcagccgga agcggtgcac tggctgccac caatggtggt tacttcaatc tttccgatgg
     2281 taagagcacc agttttgtgg tgttggacgg acgtctggtt gccgatccaa gagagaacaa
     2341 agctctaact ggcaatccca ggctgactgc ctttctgcct aagatttatg agcgcagcga
     2401 attgcgcgtc tatcaagata agctaggcaa aatttcttat gccatcgccg ctcatagtga
     2461 tgctgttcct gctgctctca cactcaagca ttctctgcag ggaggtccgc gccttctgcc
     2521 gcagttgacc gccgaagctg aggccttcct gcgcac
//
LOCUS       DAOQNZ010000521         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23491_length_2551_cov_3.397837, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000521 DAOQNZ010000000
VERSION     DAOQNZ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23491_length_2551_cov_3.397837"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKW73_09975"
     CDS             <1..436
                     /locus_tag="PKW73_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73656.1"
                     /translation="LPVAMTGCKSRPAAETGAATTGEKVETMETIPAPKSPHRVMAAV
                     AGNDSLSPLQLQYLVNMQNKLLTAWRPVPSDKQYSVTCEFNVTRPGFCSNVQVVAASA
                     NQRSIEKTLDTVRMQAPFGALPSDFKEAPVTFRCDFVYNPGK"
     gene            408..2057
                     /locus_tag="PKW73_09980"
     CDS             408..2057
                     /locus_tag="PKW73_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73657.1"
                     /translation="MTLFTTQANRLKFVPALSSRAWDLSYWAAVFGTAVLTLVAVIAS
                     ISHPLLGQPDQCMYLSMADLLMAGRLPYVDCFDNNPPLIIYLGVIPMVVAKLAALPAT
                     LAFALCVHVLAAFSAGLSFWLLRSTRGKVSGLLACLSVLVFAWFNAFQEMDLGQREHI
                     FTLAVFPYLLLRFLRYRLEPDLSDCDKVGAISACLTGLLAGVGISLKHYFLFIVLMVE
                     LACFLRRPCGRSLLHLEVATCLAAITLYLLHFLFLPPSMLSGFFGFVLPIYARGYDYY
                     TNSFLFNLATYWRPDFLQLGLVSLLALFLAAVSFEITGLLSLFLTFAWSSALVFFLAG
                     QAWQYHLLPVRLGTCLTLSAGAGLLLVCLLRRREKYFSWVFVFVCLTIACHSALTLKQ
                     RLQAEEEQHEHFDMAFLGHKGTCFDYEVSPAVATIIANTRKTDTVLFISTAMAPGYPS
                     MIQTERRPGSRFIHAMPLVVLDYVVTSKSGFDRPRFQKMEEQVLSWYGEDIAGFKPRL
                     IFIQVSPMAGVLEKLNFQKRFLGAYELIGEDFGMQIWRRKD"
     gene            2057..>2551
                     /locus_tag="PKW73_09985"
     CDS             2057..>2551
                     /locus_tag="PKW73_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73658.1"
                     /translation="MSSSSLTKRPLRQLRKEQRPALVFALVVALFQSAFALNLSPTTA
                     YQLSAAAAVLNGAVPFVSFYFDELPTQIVVRLPEVLLSWLGQYFPGSNGGAQLAIAHY
                     LLTQVYQFFVFAFCLRIWRHSPGSSGGRPLLYLLAFMGAQLPFVFQLGCVQHLVFLGH
                     LPLVF"
BASE COUNT          448 a          698 c          652 g          753 t
ORIGIN      
        1 cctgcctgtg gcaatgaccg gttgtaaatc gcgaccggct gccgagaccg gcgcagccac
       61 cacgggtgag aaagtggaaa ccatggagac catcccggcg cccaagagcc ctcacagggt
      121 catggctgcc gtggccggta atgacagtct cagccccctg caattgcagt atctggtcaa
      181 tatgcaaaac aagcttttga ccgcctggcg cccggtgcct tccgacaagc agtacagcgt
      241 cacctgtgag tttaatgtga ccagaccggg cttctgttcg aatgtacaag ttgtggctgc
      301 ttccgccaat cagcggtcta ttgaaaagac ccttgatacg gtgcgcatgc aggctccctt
      361 cggcgcccta ccctccgatt tcaaagaggc tccggtgacc ttccgctgtg actttgttta
      421 caacccaggc aaatagactt aagtttgtgc ccgctttgag cagtcgcgcc tgggatttat
      481 cctactgggc ggcggttttt ggcaccgcag tgttaacact ggtggctgta attgcctcca
      541 tcagccatcc tttgttgggg cagcccgatc agtgcatgta cttgagcatg gccgatttgc
      601 ttatggccgg gcgattaccg tatgtggatt gctttgataa caatccgccc ctgataatct
      661 atttgggcgt gataccgatg gtggtggcaa agctggctgc cctgccggct actctcgctt
      721 ttgccctgtg cgttcatgtt ctggcggcgt tttcggcggg cttgtcattc tggttgcttc
      781 gttccacccg aggcaaggtt tccgggcttc tagcctgtct ttccgtgctt gtttttgcct
      841 ggttcaatgc ttttcaggaa atggaccttg gtcaaaggga gcatattttc acccttgctg
      901 tttttccata tctcttacta cgctttcttc gttaccggct tgagccggac ctctccgatt
      961 gtgacaaggt cggcgccatt tctgcctgct tgaccggcct gcttgccggt gtaggtattt
     1021 ctctcaagca ctactttctc tttatagttt tgatggtgga actggcctgc ttcctgcgcc
     1081 gaccctgtgg gcggtcgctc ctgcatttgg aagtggcaac ctgcctggct gccatcaccc
     1141 tttacctctt gcacttcctg tttttgccgc cgtccatgtt gtccggtttt tttggttttg
     1201 tgctgcctat ttatgccaga ggctacgact attacaccaa ttcttttctc ttcaatctgg
     1261 ctacttactg gcgcccggac ttcttgcaac ttggattggt cagcctgctg gccctctttc
     1321 tggcggcggt aagctttgag attacgggtc tgctcagcct ttttctcacc tttgcctgga
     1381 gctccgcgct ggtctttttt ctggccggtc aggcctggca gtatcacctt ttgccagtcc
     1441 ggctgggcac ctgtcttaca ctcagtgccg gcgccggttt actgcttgtc tgcctcctga
     1501 gaaggcggga gaaatatttc agttgggttt tcgtttttgt ctgtctgact attgcttgcc
     1561 attccgcact tacgctcaag caacgccttc aggctgaaga agagcagcat gagcacttcg
     1621 atatggcttt ccttggccat aaaggcacct gttttgacta tgaagtcagc ccggcggtgg
     1681 ctaccataat agccaacacg agaaaaacag atactgtgct ttttatttct acggccatgg
     1741 cgcccggtta tccttccatg attcagaccg aaaggcgccc cggttcacgt ttcattcatg
     1801 ccatgccgct tgtggtactt gattatgtgg tgacaagtaa aagcggcttc gaccggcctc
     1861 gctttcaaaa aatggaagag caagtcctgt cctggtatgg agaggatatt gccggattca
     1921 aaccccgact tatctttatt caggtgagcc ccatggccgg ggtgctcgaa aaattgaatt
     1981 ttcagaagcg ctttctgggg gcttatgagc ttatcggtga agacttcggc atgcagatct
     2041 ggcggaggaa agactgatga gttcttcctc cctcaccaaa agaccgcttc gacaattaag
     2101 gaaggagcag agaccggccc ttgtctttgc ccttgtggta gcactctttc agtcggcctt
     2161 cgctctcaat ctttcaccga ccacggccta tcaactgagc gccgccgcgg ccgttttgaa
     2221 tggagctgtt ccttttgtta gtttctactt cgacgaattg cccactcaga tagtggtcag
     2281 attgccggag gtacttctgt cttggctcgg gcaatacttc ccgggaagca acggcggtgc
     2341 tcagttggcc atcgctcatt atctccttac ccaggtctat cagtttttcg tttttgcttt
     2401 ctgtctgaga atctggaggc actctcccgg ttcgtctgga ggtcgtcctc tcctttatct
     2461 gcttgccttt atgggggctc aactgccctt tgtctttcaa ctgggctgtg tacagcatct
     2521 ggtctttctg ggtcatctgc cgcttgtatt c
//
LOCUS       DAOQNZ010000522         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23495_length_2551_cov_2.754006, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000522 DAOQNZ010000000
VERSION     DAOQNZ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23495_length_2551_cov_2.754006"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PKW73_09990"
     CDS             complement(<1..1101)
                     /locus_tag="PKW73_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNA73659.1"
                     /translation="MSRLALVRDPRFQKHQTPDAHPETPHRLVAIDNVISRFSLEHGL
                     TELPPRAAETGQIAAVHKPSYIEELHENALKLSGEELIKLDGDTFMGAESFDTALLAC
                     GAGLVAIDAVKGGEVQSAFVAVRPPGHHALADKPMGFCLFNNVAVAARYAQTRGYQKV
                     FIIDWDVHHGNGTQWAFYDDPSVFFTSFQQYPFWPYDSGWFTEDGLGDGRGHNMNIPL
                     PRGTGDRGYLKAFDRLVKPLCEAFAPDLIMVSAGYDAHQDDPLGQQRISTAGYAMLSQ
                     RVVDLGRALGVPCVVFLEGGYNVKSLSESAVATMRVLNARNDSELGEVHASYLMPYAA
                     ASSNHITGDSSEHQVDERIDEVRRHFAKYWRVL"
     gene            1673..2464
                     /locus_tag="PKW73_09995"
     CDS             1673..2464
                     /locus_tag="PKW73_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73660.1"
                     /translation="MQSPSPGKKPFVILVTSTQGGSGRSLIASALATHLTRFGEVALM
                     GDDVAGRGYFGSAVLHPGSRNILSAAPEIIAQATNFGYHLNRLRLIEQIGETQPLPER
                     VAAVKPFHQFVILCAESRLESTTDFTKARSRSEMVDLTIPVYRQTERSHYLTSMLQEI
                     AKMCELYLPVANFLSNAKEVDLGSHWTLEALKTFGECFNQLLKVCPNLHMRDPESIFH
                     GTAFSYTPVLSYGSVNPMFKFSATAPGTPANAISNIANIVLAAAG"
BASE COUNT          646 a          750 c          621 g          534 t
ORIGIN      
        1 caagacgcgc cagtacttag caaaatgacg gcgaacttcg tcgatccgct cgtccacctg
       61 atgctcactg ctgtcaccgg ttatgtgatt gctggaggct gcggcatagg gcatcagata
      121 ggaggcatgt acctcaccca actccgaatc attgcgggca ttcaaaactc gcatggtggc
      181 cacggccgat tcggagagag acttgacgtt atagccgcct tcaagaaaaa cgacacaggg
      241 cacgcccaga gcccgtccca gatcaaccac ccgttgactg agcatggcat aaccggccgt
      301 ggagatgcgc tgttgaccga gaggatcgtc ctggtgtgca tcgtatccag ccgaaaccat
      361 gatcagatcg ggcgcaaacg cctcgcacag aggctttacc aggcgatcga aggccttcag
      421 atagccccgg tctcccgtgc cccgcggcaa gggaatattc atgttatggc cgcgcccatc
      481 acccagtccg tcttccgtaa accagcccga atcgtaaggc cagaaagggt attgctggaa
      541 ggaagtaaaa aagacactgg gatcatcata gaaggcccac tgggtgccat taccatgatg
      601 aacgtcccag tcaatgataa agaccttttg gtagccccga gtctgggcgt aacgagcggc
      661 cacggccaca ttgttgaaca agcaaaaacc catgggctta tcggccaggg catggtgacc
      721 aggcggacgc accgccacga acgccgactg tacttcaccg cccttaactg catcaatcgc
      781 taccagaccg gcaccgcaag ccaataaggc agtatcaaag ctttcagccc ccatgaaggt
      841 atcgccgtcc agcttgatca gttcctcccc gctcagtttg agggcattct catgcaattc
      901 ctcgatgtag gaaggcttgt gcacagccgc aatctgcccg gtctcagccg cacggggagg
      961 cagttccgtc agaccgtgct cgaggctgaa acggctgatg acattgtcta ttgccaccag
     1021 gcgatgggga gtttccggat gggcatcggg agtttgatgt ttctgaaacc tgggatctct
     1081 tacaagcgct agcctggaca tggggacctc ttgggagtga tgtaagtgtc gtcgcttcgg
     1141 cactgccgct cgacctgact gagcctgcca gtcctgtccc aagcaccgag ccggggacag
     1201 gaacacccta ttatattgcc agcaagccct cagttagtgg accgaccaca agataactgc
     1261 aaggttaatc ggactatttc tgacaaccca aaacatctct ttcaaaccgt tcgcaagtac
     1321 ttcgccattg tgtcggaacc ggggctaatt acaggccacc ccgggataga tttctgagcc
     1381 gccgccaaca gatcaaaacc tgtcatatat aaaaactgaa attctagctc cctattacta
     1441 attaaataag agcctaagcc tccaagaagg attgtcgcgc ttcaaagata ttactcatat
     1501 tgaagtagca aaccaaacca tttctttttt cacagggtcc tcacagccaa agcaaatcgc
     1561 atcagcccta acaaggaaaa cccttccatc ttttcgatgg tcgggtcaac tttgcttgca
     1621 aaagtgcctt tgccgacagc tgtcttcgac tccccaaacc aagaggttta ccatgcaatc
     1681 tccttctccc ggcaagaaac cctttgtaat tctcgtaacc tcgactcagg gtggttccgg
     1741 ccgctccctt atcgcctcag ccctggccac gcatctgacc aggttcggcg aggtagcttt
     1801 gatgggcgac gacgtggccg gtcgcggcta tttcggctca gcggtacttc atccaggcag
     1861 ccgaaatatt ctctctgcgg cgccggaaat cattgcgcaa gcaacgaatt tcggttatca
     1921 cctgaaccgg ttacggttaa tagagcaaat cggagaaacc caacctctgc ctgagagagt
     1981 ggcagccgtc aagcctttcc accagtttgt catcttgtgc gcagagtcca gactggagag
     2041 cacgactgac ttcaccaaag cgagaagccg gtccgaaatg gtcgatttga ccattcccgt
     2101 ttaccgtcaa accgagcgca gccactacct gaccagcatg cttcaagaaa tagcgaagat
     2161 gtgcgaactc tatctgccgg tggcaaactt tctgagtaac gccaaggaag tagacctggg
     2221 aagccactgg accctggaag ctctgaaaac tttcggagaa tgcttcaatc agctgctcaa
     2281 ggtctgtccg aacctgcaca tgagagaccc tgagagcatc ttccacggca cggccttcag
     2341 ctacacacca gtgctgagtt acggctccgt caatcccatg ttcaagttct cggcaaccgc
     2401 cccaggaaca ccggcaaacg ccatcagtaa catcgccaac atcgtcttgg ccgctgccgg
     2461 ctgaagcagc ccgaccaagc ccctattttt caactacgga gaaatcaaat gaccaagacc
     2521 ttaacccaaa actacctcaa tctgaacggc t
//
LOCUS       DAOQNZ010000523         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23501_length_2550_cov_6.333066, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000523 DAOQNZ010000000
VERSION     DAOQNZ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23501_length_2550_cov_6.333066"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKW73_10000"
     CDS             <1..461
                     /locus_tag="PKW73_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73661.1"
                     /translation="LGLYSATPAIVFRSGYQDLPSQTQAEWAYDLHDFVVARGGGLEN
                     YAKYVEEQAERLKRPQITAYRTFSKELPDPYDPLERCYVSYADRKKFADEWWTARDEG
                     YNTALAKDWWLNDAILDGTNLWHRWEMTYLRSADREAMRTAELNQLVKKK"
     gene            648..1925
                     /locus_tag="PKW73_10005"
     CDS             648..1925
                     /locus_tag="PKW73_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73662.1"
                     /translation="MSDAKAKDQGDQHQAKLEVRPMQSADAESVRLAGRVEMNIVEQA
                     RSNASDGSLRHYQSTTQVRRNLPSDWQQQNQSIELIDGDVTVSRTNPLKEANLTKGAN
                     ADGKPYKVYTAQAGQALEKPGDIDKRLTEGDYQSSVKLNVQITNVPEVSAGIKPDELL
                     GYVGQVMEAGAKAVRPIEEHLSKADSVNQDLWNLPGNVGKVFEHLGQSPEQLNQDVAV
                     VTGRILETIDKPMTAEQRSEAAGALMPMFFFEGGGKPIDKAAVNQMKLDQMTAEELKM
                     LGIDRVQMEMPAIPEDLKDLPLTKASREFLDAIERKNRVIEIGEPNSWVVSHLDSIPA
                     EASVFVTGPLEADIILLRENPSKIAALEEFLHGTQRQIANLKDQDRVILELHVKDFMI
                     RHRRMLGLSDEDVQMLEALKLEELKRAEMQGYR"
     gene            1933..2331
                     /locus_tag="PKW73_10010"
     CDS             1933..2331
                     /locus_tag="PKW73_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73663.1"
                     /translation="MKESNYQLFFRVVDHYVIKGNLILVSDRTDSGRARTLETIQIKD
                     PSGANARFYTAGCVSYWQMPGDKPAPFCMAIKGLKPEEIPIGSTVWLEKREIADQDLS
                     RYRKLKKAPPPMPGGLPEVTQQQGGKKQAV"
BASE COUNT          779 a          529 c          701 g          541 t
ORIGIN      
        1 cattggggct gtattcggct actcctgcca tagtgtttag atccggctat caagacttgc
       61 caagtcaaac acaagcagag tgggcttatg acttgcatga ctttgtggta gctagaggcg
      121 gcgggttaga gaactatgcc aagtacgttg aagagcaagc ggagaggcta aagagaccgc
      181 agataaccgc ttataggacc ttctcaaaag aactacctga cccgtatgac ccactggaac
      241 gttgttacgt ctcatatgca gaccggaaaa aatttgctga tgaatggtgg acggcaagag
      301 atgagggcta caacacggct ctagcaaagg attggtggct taatgacgcc atactagatg
      361 ggacgaactt gtggcaccgt tgggagatga cttatctaag aagtgcagac agagaggcta
      421 tgcggacagc ggagttaaac caattggtca agaagaagta aaaccggtgc cgggactgtc
      481 gaagcggacg ggcttgcttc ggcttacttg cgagccttcg ccgcccaggg agcagtcaat
      541 tagagcctgt cgagggatcg taactccccg ctccaccgct tagctggttt cttatcagtc
      601 acagacgggc ataatatgca ccagtaatgg gcgctcaggg ggctgctatg tcagacgcca
      661 aagcaaaaga ccagggcgac cagcaccagg ctaaattaga agtacggcca atgcaatccg
      721 ctgatgccga gtccgtgcgc ttagctgggc gtgtagagat gaacatagtt gagcaagcac
      781 ggagtaatgc tagcgatgga tcactaaggc actatcaatc aacaacgcag gtacggcgaa
      841 atctcccctc tgactggcaa cagcaaaatc agagtattga gctaattgac ggtgatgtga
      901 ctgtttcaag aaccaaccca ttgaaagaag cgaatctaac caaaggcgcc aatgctgatg
      961 gtaagcccta taaggtgtat acagcccaag cggggcaggc gctagaaaag ccaggggata
     1021 ttgataaaag gctgacagaa ggggattatc aatccagcgt caagctaaat gtgcagataa
     1081 caaacgtgcc ggaagtttca gcaggaatca aaccagatga gctgctaggg tatgtcgggc
     1141 aggtcatgga agctggagca aaggccgtaa ggccaattga agaacaccta agtaaggcag
     1201 attcagtaaa tcaggactta tggaacctgc caggaaatgt aggcaaggtc tttgagcatc
     1261 tagggcaaag tccagagcag ttgaaccagg atgtggcagt agtgacaggt cgaatcttgg
     1321 agacaataga caagccaatg acagcagagc agcggtctga agcggccggg gctctaatgc
     1381 cgatgttttt ctttgaaggt ggcggcaagc cgatagacaa agcggcagta aaccaaatga
     1441 agctcgatca gatgacagcc gaagagctta aaatgcttgg tatagacaga gtgcagatgg
     1501 aaatgcctgc cataccagag gatttaaaag acttgccact gaccaaggca tctagagagt
     1561 ttttggatgc tatcgagcgc aaaaataggg ttatagaaat tggtgagcct aattcctggg
     1621 ttgtgagcca cttagactcg atacccgctg aggcttcggt atttgtcaca ggaccattag
     1681 aggcagacat aattttgttg cgagaaaacc ccagtaaaat agctgcttta gaggaatttt
     1741 tacacggaac gcagagacag atagctaacc tgaaagacca agaccgtgtt atcttggaat
     1801 tgcatgtcaa ggatttcatg attcggcacc gcagaatgtt aggtctcagt gatgaagatg
     1861 tacagatgct ggaagctctg aagttagagg aattgaagcg ggccgagatg caaggctata
     1921 ggtaggtaag acatgaaaga atcaaactat caacttttct tccgggtagt agaccattac
     1981 gtgatcaaag gcaatttgat actggtttcc gatagaaccg attctggacg cgcaagaact
     2041 ctagagacta ttcagataaa agatccaagc ggtgcaaatg caagatttta taccgctggc
     2101 tgtgtcagtt actggcaaat gccaggtgac aagccagccc cattttgtat ggcaatcaaa
     2161 ggattaaagc ctgaggaaat accaataggc agtacggttt ggttagaaaa gcgagaaatc
     2221 gccgaccaag acttgagccg ttatcggaaa ctcaagaagg caccgccgcc tatgccagga
     2281 gggttaccag aggtgacgca gcagcagggc ggaaagaaac aggcggttta gttatcggtc
     2341 gcaattgaag cgggataagg ctctttgggt accccaaata agggttaggg tataaatgac
     2401 aaccgtgggc ggtggcacaa gtcaagcgtt ggagataaat gaaagctcgg aaatgcccac
     2461 ccagtaatca atacaatctg tctatataaa acaacgccac ataatatata ttatcggact
     2521 ggtttgtatg ccgggaggct tcaatctctt
//
LOCUS       DAOQNZ010000524         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23506_length_2550_cov_4.186373, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000524 DAOQNZ010000000
VERSION     DAOQNZ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23506_length_2550_cov_4.186373"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKW73_10015"
     CDS             complement(<1..551)
                     /locus_tag="PKW73_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73664.1"
                     /translation="MTDKAILGADLISGLISTLGKQVVSLKLQLASLQAHLSSSSGLE
                     KSVKSLLPERGHLDLGQVPGLIMRLSKERSLGAALSGDDGQILMASPAFYSLTGLKLD
                     DPSHSTVRFYREEAGVEKECENLPWILALPNLRGGGEATVLFGSRYLVRQPEQEGGER
                     WLQFVFRPLTGDGGGCLIFVAEAT"
     gene            complement(648..1205)
                     /locus_tag="PKW73_10020"
     CDS             complement(648..1205)
                     /locus_tag="PKW73_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNA73665.1"
                     /translation="MDSKDASQTQLNRVLSQKILRARARKLARPVFAEAQKELAYRVI
                     MVNLGGGRFAFESEYVEEVVRLKEVVPLPGLSAPFVGLTNVRGELVVVMEPGQMLAPG
                     QSSASYSSVQKRLAVVLVHRGRRLAVLVEDLLGSRDVDSSELAREFSGLSSQLSLLTR
                     AVTRELLIILDVAKIFEEVRGAAGG"
     gene            complement(1220..2524)
                     /locus_tag="PKW73_10025"
     CDS             complement(1220..2524)
                     /locus_tag="PKW73_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNA73666.1"
                     /translation="MGSDLTKDQQHRLATWVSGNLGLSYPPERQADLARAFLRYAESK
                     GEAPERLVEKLLDGAAYGEQLAELAPYLTVGETYFFREPATFRLLSAEILPALLQGRD
                     LKQPTVLNIWSAGCCTGEEAYSLAIFVGHWLSALGAAAKVSVLGTDINQEFLEKARRA
                     VYKSHSFRTRLAELACYEPYFERDGDSFRVRADIAGLVRFAIFNLAGEQELPSCDSPS
                     AGFDLIICRNVLIYFSAEKQIAALKKLAARLAPGGYICLAPCEIPANLESDLPELERV
                     EVGGRFFLRSVPAEKAGAAGSAGLTGSLETSFQALTLRYHLYVNTGRFQEAIVCLNQY
                     LANTEDSRALYLRALVEEETGALGEAEASLLRCLEQQPDFHMARFLLASGYLARGKRP
                     QGVELLQHLRQSLAQVERDSLLPFSEGITASELLSVIDSLLQ"
BASE COUNT          656 a          772 c          624 g          498 t
ORIGIN      
        1 gtggcttccg ctacgaaaat cagacagccg ccaccatcac cggtgagcgg tcggaacaca
       61 aattgtagcc agcgctcgcc tccctcctgc tcgggctgac gcaccaggta gcggctaccg
      121 aagagcacgg tcgcttcccc gccgcctctc aaattaggca gagctagaat ccacggcaag
      181 ttctcgcatt ctttctcaac cccggcctct tcccgataaa atcgcaccgt ggaatgagag
      241 ggatcgtcca gcttgagacc ggtcaaactg tagaaggccg gactggccat gagaatctga
      301 ccgtcatcgc cggaaagagc agcacccagg ctgcgctctt tcgaaagacg cataatcagc
      361 cccggtacct gacccaggtc aagatgaccc cgctccggca aaagggactt cacggatttt
      421 tcaaggccgg aactggaaga aagatgcgcc tgtaaggagg ccagttgcaa tttgagcgat
      481 actacctgct tacccaaagt agaaattaag ccggaaatca gatcagctcc cagaattgct
      541 ttatcagtca ctttcagccc ccgcgcctct aaccttcgaa cacgcccaac aaaaacttac
      601 ctcaggcctt gaagatatac ccttccatat ttgcagttaa ccctgagtta gccaccggcc
      661 gctcccctga cctcttcaaa tattttggct acgtccagaa ttatcaagag ctccctggtc
      721 acggctctgg tcaagagtga aagttggctg gaaagtccgg agaattcgcg agccagttcg
      781 gaactatcga catctcggga gccaagcaaa tcttccacca gaaccgccag gcgccgtcca
      841 cggtgcacca gcacgacggc cagacgcttc tgcacactgc tgtaggaagc actgctttgt
      901 ccgggagcaa gcatctgtcc aggctccatc actaccacca attcaccccg tacattggta
      961 aggccgacga agggagcgct gagaccaggc aggggtacta cctccttcag tctgacaact
     1021 tcctccacat actcgctttc aaaggcgaag cgaccaccgc caagattaac catgatgacc
     1081 cggtaggcca gctctttttg agcctcggca aataccggcc gagccaactt gcgcgcccga
     1141 gcacgcagta ttttctgcga cagaacacga ttgagctgag tttgagaagc gtctttagaa
     1201 tccactgaaa agacccgatc tattgcagta aggaatctat cacagataaa agctcagagg
     1261 cggtaattcc ctcggaaaag ggcagcaaag aatcgcgctc gacttgggcc agtgactgcc
     1321 ttaaatgctg caggagctcc actccctgag gacgtttgcc ccgggccagg taaccactgg
     1381 ccagtaaaaa gcgcgccata tgaaaatccg gctgctgctc aaggcaacga agaaggctcg
     1441 cctccgcctc gccgagggca ccggtctcct cctccacaag ggctcgcaaa tatagcgccc
     1501 ggctgtcttc agtattagca aggtactgat tgagacagac aatcgcttcc tgaaagcgac
     1561 cggtattgac atagaggtga taacgcaagg taagagcctg gaagctcgtc tcaagactac
     1621 ctgtcaaccc tgcgctgccg gctgctcccg ccttctcagc cggcacagac cgaagaaaaa
     1681 agcgaccacc cacctccact ctctcaagct ctggcaaatc gctttccaga ttagccggaa
     1741 tctcgcaggg agcaaggcag atataaccgc ctggagccag tctggctgct aatttcttga
     1801 gggcggcaat ctgcttctcg gctgaaaagt agatgagaac attgcgacag ataataagat
     1861 caaaaccagc ggagggactg tcgcaagagg gcagttcctg ctcgccggcc agattgaaaa
     1921 tggcgaaacg caccagtccg gcaatatcgg ctcgaacccg gaaactgtca ccgtcccgtt
     1981 cgaaataggg ttcatagcat gccagctccg ccagtctggt ccgaaaggaa tgactcttgt
     2041 aaacagcccg cctggctttc tccagaaact cctgattgat atcggtgccc agtaccgaaa
     2101 ctttcgcagc cgcaccgaga gcggagagcc agtggcccac aaaaatagcc agggaataag
     2161 cctcctcgcc ggtgcaacag ccggcactcc agatattgag aaccgtcggc tgttttaagt
     2221 cgcgcccctg cagcaaggcg ggtagaattt ccgcgctcaa aagcctaaaa gtagccggtt
     2281 ctcgaaaaaa ataagtttcc ccgacagtta aatagggggc cagttcagcc aattgctccc
     2341 cataagcggc accatccagc aacttttcca ccagccgctc cggtgcttcc cctttagact
     2401 ccgcataacg caaaaatgcc cgtgccagat cggcctgtcg ttccggcgga taagacaagc
     2461 ccaggtttcc ggaaacccat gtagccagac gatgctgctg gtcctttgtt aaatccgatc
     2521 ccatatgcac tcctcaaggt cttatacccg
//
LOCUS       DAOQNZ010000525         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23507_length_2550_cov_3.844489, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000525 DAOQNZ010000000
VERSION     DAOQNZ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23507_length_2550_cov_3.844489"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PKW73_10030"
     CDS             <1..958
                     /locus_tag="PKW73_10030"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HNA73667.1"
                     /translation="ITTRGFQGTVPKDLKVIEAPTPFVWVIGRIKTDGPSDYGAVHAI
                     QDGLNVTPLSAWGKKAPEIVAKIDDSIDMKKPPLETVNEMTAAVYFSQAAELLKLYAP
                     HATDWSIINRMSRLGLVPGESFNLDSLPEQIQKAVAKATTDALKTMVEKTTRMGSPVN
                     GWLMNTESMGVYGNNYMKRAIVAMVGLGANQPEDAIYPLNYADSEGRPIEAQNNYVLH
                     FKKEELPPVNAFWSLTLYDKDGFQCANSLNRFAISSWMPLVKNSDGSLDIYVQNANPG
                     KEKEANWLPAPAAGVLGLTMRLYAPKPEALNGDWQPPSIKKV"
     gene            complement(970..>2550)
                     /locus_tag="PKW73_10035"
     CDS             complement(970..>2550)
                     /locus_tag="PKW73_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNA73668.1"
                     /translation="AQEPAPQPMLKEQTLRAEEAPTEQTAPQSALAHGAEVFRYNLIQ
                     APLTGLTQLADFTLGTRWESQTRFYQAPVPAAHGSAAWVAEVAGGVAAGAVPFLLLHK
                     AIGPGAVAKVEVMGDYSAAKTLPIIGRSALVGAGWGGVLTPVQEGENFWTTRLNNSLS
                     NAAAFATLTAGTVALKRSGFMFLRNDMVDGTASGALGGFVAADARALLTQGHLASGRE
                     RLHSMSTFALGGLMLGGATTAYQNFRPASGIAGVRTPEDMRVLADRTIKPGHPERYAF
                     EPEQKIPAKADALMAESKVHPEWHNRASVAVRQEIDSSNMPVDAKKLVVSGFQEMSYG
                     LEALHSRPQQKPIMVVYGSARFKENDFRYQRARYIGGRAVEEGFDVMTGGGPGTMEGA
                     NRGAYEAGGNSIGVVLKLPHEKRGNGYQTMTLKHRNFFTRMEMLKQGDVFVVEDGGIG
                     TAAEALDTLTHIQTGKLKEVPIYFVGKDNYKQMDSWLARMERNGTISPADRHLYRIVD
                     NPDDIFLDLERRRSQSGT"
BASE COUNT          592 a          766 c          669 g          523 t
ORIGIN      
        1 gatcaccacc aggggcttcc agggcactgt acccaaagac cttaaagtca tcgaggcacc
       61 tacacccttt gtctgggtaa tcggccgcat taaaaccgac ggaccctccg attacggcgc
      121 cgttcatgcc attcaagacg gactgaatgt gacgccccta tctgcctggg gcaaaaaggc
      181 gccggagata gtagccaaaa tagacgacag catcgacatg aagaaaccgc ccctggaaac
      241 agtcaacgag atgactgccg ccgtttactt cagccaggcc gccgaattgc tcaagctata
      301 cgccccccac gccacagact ggtcgatcat aaacagaatg agccgcctcg gtcttgtacc
      361 aggtgagagc ttcaacctgg attctctacc agagcagatt caaaaggctg tggcaaaagc
      421 aacaaccgac gctctcaaaa ccatggtaga aaaaaccacc cgcatgggca gcccggtcaa
      481 cggctggctg atgaacaccg agagcatggg tgtctacggc aacaactaca tgaaacgcgc
      541 catagtcgcc atggtcggtc ttggcgccaa ccaaccggaa gacgccatct acccgctcaa
      601 ctatgccgat agtgagggac ggccaattga agcacagaac aactacgtgc tgcacttcaa
      661 gaaagaagag ctgccgccgg taaatgcctt ctggtccctc accctctacg ataaagacgg
      721 cttccagtgc gccaacagcc tcaatcgctt tgccatcagt tcctggatgc ccctggttaa
      781 aaacagcgac ggctccctgg atatttatgt gcagaatgct aacccgggca aggagaaaga
      841 agccaactgg ctaccggcac cggccgccgg cgtgctgggt ctgaccatga gactttatgc
      901 gcccaaaccg gaagccctga acggcgactg gcagccgcct tccatcaaaa aggtctaggt
      961 ctctagtatc taagtaccgg actgacttcg acggcgctct aaatcaagaa agatatcatc
     1021 cggattgtct acaattcggt ataggtgccg gtcggcggga gagatggtgc cattgcgctc
     1081 catccgggcc aaccatgagt ccatttgctt gtagttatcc ttaccgacaa agtaaatggg
     1141 tacttccttc aacttgccgg tctgaatgtg agtgagggta tcaagggcct cggccgccgt
     1201 gccgatgccc ccgtcttcta ctacaaaaac atcgccctgc ttcaacattt ccatgcgggt
     1261 gaagaaattg cgatgcttca aggtcatggt ttgatagccg ttacctcgtt tctcatgggg
     1321 caatttgagc accactccga tggagttgcc accggcttca taggcgccgc gattagcgcc
     1381 ttccattgtg ccgggtccgc cgcccgtcat tacatcaaag ccttcctcca cagcccttcc
     1441 gcctatgtag cgggcgcgct gatagcgaaa gtcattttcc ttgaagcgcg ccgagccgta
     1501 cacaaccatt attggctttt gctgcggccg tgaatgcaag gcttccagtc cataagacat
     1561 ttcttgaaag cctgaaacca caagcttctt cgcatcaacg ggcatgttgc tgctgtctat
     1621 ttcctggcgc accgccacac tggcccggtt gtgccactcg ggatgaacct tcgactcggc
     1681 catgagggca tcggccttag ctggtatctt ctgctccggt tcaaaggcat atcgctccgg
     1741 gtgcccgggt ttgatggttc ggtcggccag gacgcgcatg tcctcgggtg tacgcacacc
     1801 ggcaataccg ctggcgggac ggaagttctg ataggccgtg gtggcgcctc ccagcatcaa
     1861 gccgcccagg gcaaatgtgc tcatagaatg aagcctctct ctaccggagg caaggtgtcc
     1921 ttgagttaat agggctctcg cgtcggcagc cacaaaacca cctagcgcac cgctggcggt
     1981 accgtctacc atgtcgttgc gcaagaacat gaaaccggag cgcttcaggg ccacggtgcc
     2041 ggccgtcagg gtagcgaagg ccgctgcgtt ggaaagagaa ttattcaacc tggtagtcca
     2101 gaaattctca ccttcttgta cgggcgttaa aactccaccc cagccggcac ctaccagggc
     2161 agacctacct attatcggca gagtttttgc agccgaataa tcacccatca cttctacttt
     2221 ggccacggct cccggaccaa ttgccttatg caaaagcaag aagggaaccg ccccggcagc
     2281 tactccacca gccacctctg ccacccaggc tgcacttcca tgagcagccg gaaccggcgc
     2341 ctgataaaac cgcgtctgtg actcccacct ggtgccgaga gtgaaatcgg ctagttgggt
     2401 caagccggtc aggggcgctt gaatcaggtt atagcgaaag acctcagccc catgtgcgag
     2461 agccgactga ggcgcagttt gctcagttgg agcctcctcc gcgcggaggg tctgctcttt
     2521 cagcatgggc tggggtgctg gttcctgcgc
//
LOCUS       DAOQNZ010000526         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23545_length_2547_cov_4.567014, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000526 DAOQNZ010000000
VERSION     DAOQNZ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23545_length_2547_cov_4.567014"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..666)
                     /locus_tag="PKW73_10040"
     CDS             complement(76..666)
                     /locus_tag="PKW73_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNA73669.1"
                     /translation="MHDCPTCKVPLHDHEQVCPSCGTPQKVKRSYSKMLGEEHRKPPV
                     NLLPIVGAIMAVIIGFIVMAQGSWVGKLMNRAPQPEDPMAKLTYLDARQIVESKITEG
                     VTAAGGTAEFKWQRGGNDVEKTSEGPVEVTCQVGLQSAEQIKPIVDPVKDYFEKAQIT
                     TLTVSDEKLHTTRTYTVTPPAPGAGAAPEPEAAPAQ"
     gene            complement(731..2413)
                     /locus_tag="PKW73_10045"
     CDS             complement(731..2413)
                     /locus_tag="PKW73_10045"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA73670.1"
                     /translation="MPQILIFRLFFLLSLACISLGIRLMQPLPWAILSILQPLIVIAG
                     VVVPLVTVKDGQLALWTGWCLLLFFHVPAIIANIQVGKAMARMDADSLETWAKIEQWL
                     LWGRPGQFWHDMHLSYALVLRERQTDANRLLDKWDPSCQPEGKEKDKIELPKNFRDTP
                     SNYRLYGQSVMWQWSEVTATYEMLKKKQANTAAISFLAARAYLENQEPLEAATCLRQV
                     HFDETIYPLDTLATNLMPFFSLTGARDELERLTRVLKATNASVPQSLIDLWRGRCEIT
                     LGAADKAASVLAAAQAGTAVPLLRKRIARFLEPLGSGETVQRSPLVDDPSVRKEVQEI
                     WQLFRRAAFIQEILSPRRKSICVNVICLLSVFTFLASANFLLPLGLTMDCPINPLTMG
                     ASYEGVVKQGQYWRLISYLFYHSDLLHIGSNLVGLYIFGRIAENIFGSSRFLAIYFVG
                     GVLSGLTHIFLSDKIAVGASGAIQAVFAACAVGIIKLKGLPPSLRSRYLSFMGGITLF
                     QILLDQFVPFIAVFAHLGGLLAGLALGTIAAVRDPSRLDGLDDEVDGTQQFI"
BASE COUNT          621 a          682 c          705 g          539 t
ORIGIN      
        1 aaaagggatt gagagtatgc ctctgaggga gcgcctgctc tatgaaaacc gcccggataa
       61 gccctgaagg gttatttact gagccggagc agcttccggc tcaggtgcag cgccggcacc
      121 gggagcagga ggcgttaccg tataggtacg ggtggtatgc aacttctcgt cgctgacggt
      181 aagtgtagtg atctgggcct tctcgaaata gtctttcaca ggatcgacaa taggcttgat
      241 ctgctcggca ctctgcaaac ccacctggca tgtaacttca accggacctt cactggtctt
      301 ctcgacgtcg ttaccgcctc tttgccattt aaactcggcc gtgccgccgg cggcggttac
      361 cccttcggta atcttgctct ccacaatctg tctggcatcc agataggtca gcttggccat
      421 gggatcttcc ggttgcggtg cccggttcat cagtttgccc acccaggacc cctgagccat
      481 gacgatgaag ccgatgatta ccgccattat ggcgcccaca atcggcaaca aattcacggg
      541 cggcttgcga tgctcttcgc ccagcatctt gctgtaactg cgttttacct tttgaggggt
      601 gccgcaggaa gggcatactt gttcgtgatc atggagcggt actttgcaag tcggacaatc
      661 gtgcatggct gtctcacttt agggacctta taaatctagc actttcttta gcccacagcc
      721 tagcggccca tcaaataaac tgctgggtgc catccacttc atcatcgagg ccgtccaggc
      781 ggctgggatc gcgcaccgca gcaatggtgc ccagggccag gccggccagg agcccgccca
      841 gatgagcaaa aacggcgata aagggaacga attgatcaag caagatctgg aagagggtga
      901 tgccgcccat aaaactcaag tagcgcgatc tgaggctggg agggagtcct ttcaatttga
      961 tgatacccac ggcacaggcg gcaaagacgg cctgaatggc gccggatgca cccacggcta
     1021 tcttatcgga aagaaagata tgtgtgaggc cgctcaggac accgccgaca aaataaatag
     1081 ccagaaagcg gctggagcca aagatatttt cggcaatgcg cccgaaaata tagagtccca
     1141 ccaggtttga tccgatatgc agcaaatcgc tgtgatagaa gagataactg atcaaacgcc
     1201 agtactgccc ttgcttcacc acaccttcat agctggcgcc catggtgagg ggattaattg
     1261 gacaatccat ggtcagtccg aggggcaaga gaaaattagc tgaagccaga aacgtgaaca
     1321 cagagagcag gcaaatgacg ttaacacaaa tagacttccg tcttggtgaa agaatctcct
     1381 gaatgaaggc cgctcgcctg aacagttgcc agatttcctg cacttcttta cgcacggacg
     1441 gatcgtccac aagaggcgac cgctgcactg tttcgccgga gccaaggggc tccaggaaac
     1501 gagcaattcg cttgcgcaag agaggcacgg cagtgccggc ctgggccgcc gccagaaccg
     1561 aggccgcctt gtcagccgct cccagagtga tttcgcagcg tccgcgccag aggtctatca
     1621 gggattgcgg cacggaagcg ttggtggctt tgagaactct cgtcagtctc tccaactcat
     1681 ctctcgcccc ggtcaaacta aaaaagggca tcaagttggt agccagcgta tcaagagggt
     1741 aaatcgtctc gtcaaaatgc acttgccgaa ggcaggtagc agcctccagc ggctcctgat
     1801 tttccaggta ggcccgggcc gctagaaaag aaatagcggc agtattagcc tgctttttct
     1861 tgagcatttc gtaagtggct gtaacctcag accactgcca catgacggac tgtccataga
     1921 gcctgtaatt ggatggagta tcacggaaat ttttcggtag ttcgatcttg tctttctctt
     1981 tgccctcagg ctgacaagac gggtcccatt tatcgagcaa gcgattggca tccgtctgcc
     2041 tttctctcaa gacaagggca taagaaaggt gcatgtcgtg ccagaattga ccgggtctgc
     2101 cccagagcag ccactgctca atcttggccc aggtttcaag actatcggca tccatgcggg
     2161 ccatggcctt gcccacctgt atattggcga tgatggccgg cacatggaag aagaggagca
     2221 ggcaccagcc cgtccacaag gccaactgtc catctttgac agtcaccagg ggcacgacca
     2281 cgccggctat cacaatcaga ggctgcaaaa tgctcaaaat tgcccagggc agtggctgca
     2341 tcaggcgaat cccgagagat atacaagcca gactgagcag gaagaagaga cgaaaaataa
     2401 gaatttgggg catcgcttca gctattgccg gtgagggagc gtgacgcttc actgccgccc
     2461 ttctgactgt ggaaggctgg agtacgagcc aggtgagatg gaatagcctt gacaaagcgt
     2521 tcggtaataa gttgcggtct ggtgatg
//
LOCUS       DAOQNZ010000527         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23591_length_2545_cov_3.791968, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000527 DAOQNZ010000000
VERSION     DAOQNZ010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23591_length_2545_cov_3.791968"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKW73_10050"
     CDS             <1..445
                     /locus_tag="PKW73_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73671.1"
                     /translation="PETALTAESEATRFQRPLGLAFLSLLLVLCALLFVRGLSPTPPG
                     LKRNVDAAYLKSRSEPTQTGVEPPPVVKKPAPVSRISNNTAVAAGQGARARRRRPVSP
                     ARSEGERELLKKNPPNSSSGPVKQTKFDILRQMKNPALRKEAGDN"
     gene            506..2218
                     /locus_tag="PKW73_10055"
     CDS             506..2218
                     /locus_tag="PKW73_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA73672.1"
                     /translation="MNRMAISTKLKVMACTLALAVSSGYSGAMAAWYDNENTFLDRGK
                     QSYNSGAFDQAISQLSECLKLNPNRADAYYWRAMAYQGIKQQDKALKDLDEAIRLAPD
                     SATCYLNRGLIYSNQGKLEAAVEQFEEALRYDGNLNEAEINKKFCLKEIEKQRIAKES
                     PQPTQPSTPVKSSVLAVTASNTTAARAGAPRTAGSTTAAATALGNDPKWLKLEKELEV
                     KAQKERAEAERLALARAKAEAENARLALKAKEVEERAEREKARHDEAEARRIAMAAKV
                     KGKTGTATETRGAAETAGEETAGAALDITNRPVRDKWALIIGISNFQDKNLNLKYPAK
                     DARDFHDFLIKEANFAPDHVKLLVDEKATRANILSELGDKWLPRVANPDDLVLIYISS
                     HGSASDMDIGGVNYLLAHDSQVDSLYASGLPMQDLTRIIKGRVHSDRVVLVLDACHSG
                     AAEAAGSKGLYRKGNLDAEAVAQGTGQLVISSSQPSQVSWESKNYQNSVFTRCLIESL
                     RKNGSATTLGDAFQNLKDRVQEEVLRERGVMQTPVLKTRWKGNDLRLSTPAVSPRPGL
                     TDTP"
     gene            2267..>2545
                     /locus_tag="PKW73_10060"
     CDS             2267..>2545
                     /locus_tag="PKW73_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73673.1"
                     /translation="MSKVLSVPKVEEILVLARQAEGTAANAPAETERSQDLLTLLSRL
                     CAALDCEEERRDALACLHGLAAALEKENLPALQLKTVSLPGLDCPIRLL"
BASE COUNT          596 a          686 c          719 g          544 t
ORIGIN      
        1 gccggagacc gctctgacgg ccgagtcgga agccactcgc ttccaacgtc ccctcggtct
       61 ggcctttctt tcccttctcc tggttctctg tgctcttctc tttgtgagag gcttgtctcc
      121 gactcccccg ggactcaaaa gaaatgtgga tgccgcttat ttgaagagcc ggtccgaacc
      181 gacccagaca ggcgtggagc cgccgccggt cgtcaagaaa ccggcgccgg tcagccgcat
      241 cagtaacaat accgctgttg ccgccggtca gggggcgaga gcacgccgtc gccgtccggt
      301 ttcgcctgcc cgcagtgagg gggagcggga attgcttaag aaaaacccac ctaattcttc
      361 ttccggtcct gtaaaacaaa ctaaattcga tatccttcgg caaatgaaaa acccggcact
      421 gaggaaggaa gccggcgata attgataaag tgggactaat ttaggataga gcaggcgctt
      481 tgtgaagcgg agttcggcgt taactatgaa caggatggca atttctacca agttaaaagt
      541 gatggcttgt accctggccc tggcggtcag ctcgggttat tccggagcga tggcggcctg
      601 gtatgacaat gagaatacct ttcttgatcg cggcaagcag tcttacaaca gtggtgcttt
      661 cgatcaggcg atttcacaat tgagcgagtg tctcaagctc aatcccaatc gtgccgatgc
      721 ctattactgg cgggctatgg cttaccaggg catcaagcag caagacaagg ccttgaaaga
      781 tctggacgag gctattcgcc ttgctcccga tagcgccact tgttatttga acaggggctt
      841 gatttattcc aatcaaggca aactggaagc tgctgttgaa cagttcgaag aggctctgcg
      901 ctatgacggt aatctcaatg aagccgagat caacaagaag ttttgcttga aagaaattga
      961 aaagcagcgc atcgccaaag aaagtccgca gccgactcaa ccgtcgacgc cggttaaaag
     1021 cagtgtcctg gccgtgacgg caagcaatac taccgccgct cgcgctggcg ctcctcgcac
     1081 cgctggtagt actactgccg cggccaccgc tctcggtaac gaccccaagt ggttgaagct
     1141 ggagaaagag cttgaagtga aggcccagaa agagcgggct gaagccgagc gcttggctct
     1201 ggccagggct aaggctgagg cggagaacgc ccgcttagcc ttgaaagcta aagaggtgga
     1261 ggaaagagcc gagcgcgaga aagctcgtca cgatgaagcc gaagctcgcc gtattgccat
     1321 ggccgcaaag gtaaaaggca aaaccggcac cgctaccgag accagagggg cagccgagac
     1381 cgctggggag gaaactgccg gagccgctct cgatatcacc aatcgtccgg tgcgtgacaa
     1441 gtgggccttg ataatcggaa taagcaattt ccaggacaag aatctcaatc tcaaatatcc
     1501 tgccaaggat gcccgcgact ttcatgactt cttgatcaaa gaagctaact ttgccccgga
     1561 tcacgtcaag ttgctggtgg acgagaaggc cacccgcgcc aatatccttt cggagttggg
     1621 tgacaaatgg ttaccccgcg tggctaatcc tgacgacctg gtacttattt acataagtag
     1681 ccacggtagt gcctccgaca tggatatcgg cggcgttaat tacctcttgg ctcacgatag
     1741 ccaggtggac agtctctatg cctccggtct gcccatgcaa gacctgacac ggattatcaa
     1801 agggcgcgtg cacagcgacc gggtggtcct ggttcttgat gcctgccata gtggtgccgc
     1861 cgaggccgcc ggttccaaag ggctctatcg caagggtaat ctggatgccg aagccgtggc
     1921 gcaaggcacc ggtcagcttg tcatttcttc cagccagccc tcccaggttt cctgggaatc
     1981 caagaattat caaaatagtg tgtttacacg ctgtttgatt gagtctctgc ggaagaacgg
     2041 ttctgccacc accctgggcg atgcctttca gaatctgaaa gatcgcgtgc aggaagaggt
     2101 cctgcgggag aggggcgtca tgcaaacacc ggttttgaaa acccgctgga aaggcaatga
     2161 tttgcgcttg tctactccgg cagttagccc ccgcccgggt cttaccgaca cgccttaggc
     2221 tgaagtacaa taacggtcta aagttggttt gagccatcag aggaccatgt ctaaagtttt
     2281 gtccgtaccc aaggtagaag agatactggt cctggcccgt caggcggaag gcaccgctgc
     2341 caatgctccg gcggaaacgg agcggtcgca ggatttattg accctgctat cccggctctg
     2401 tgccgccctg gactgcgagg aagagcggcg ggatgctctt gcgtgtttgc atggtctggc
     2461 cgcagctctg gaaaaagaaa acttgcctgc tctgcaactt aagaccgtct ccttaccagg
     2521 gctcgattgt cccattcgtc tgctt
//
LOCUS       DAOQNZ010000528         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23676_length_2540_cov_3.573843, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000528 DAOQNZ010000000
VERSION     DAOQNZ010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23676_length_2540_cov_3.573843"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1107)
                     /locus_tag="PKW73_10065"
     CDS             complement(214..1107)
                     /locus_tag="PKW73_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA73674.1"
                     /translation="MDRNTGYCTTNLKEKETGQSFPMVIMYPTTEQEAPTRLGPYNIS
                     LAVEAAPLAGNFPLVLISHGSGGSHLVYRQLARTLAASGFIVGMPEHPGNNRNDNSRD
                     GTVENLERRPGHLSTALDWFSEDERFSPLIRTDKIAVIGHSMGAYTALAAAGGVPTAF
                     SRNEPLERSRTLTSIESDPRIKALVLMAPALVWFRPQGSLNAVNCPILLMVGEKDEIT
                     PTAYHAKLVLEGVPEPSRVIFREIENAGHFSFLSPFPKIMQHSADGALFKPAQDPPGF
                     CRESFQPILASYILEFFSGLN"
     gene            1345..2097
                     /locus_tag="PKW73_10070"
     CDS             1345..2097
                     /locus_tag="PKW73_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73675.1"
                     /translation="MPSVLQSLLQRVLSLIMPAPSLLIETTCKKLKQEALKLGSDARS
                     ALEGGAQSAAFDFSGAWDLFFELFDTARLLALKCRIWTALKEPQVTAPLTVNAMEWGA
                     CLVQLAGQFRSLQRERSGLDGETLIARQNALIERRVQANNLKERLSILERQALLTVRQ
                     KIQTDLEKAREAGNGSHKKIEKRITALLSLLNCHRYALLSEKSHWSRRNNESSDLGES
                     EFAEIAAMREASVREVLIHLNAWKDLYAGLLR"
     gene            2188..>2540
                     /locus_tag="PKW73_10075"
     CDS             2188..>2540
                     /locus_tag="PKW73_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73676.1"
                     /translation="MNVAAIKKCGSICALALCLEVFAFAALGVPVAANNETDALYGMA
                     SREMCKGLAGNHKALEHLNKALAIDPRSAKGWCLKAHILGTMEESELALPCIEKALKL
                     EPKNFVLWMEKAEIY"
BASE COUNT          568 a          625 c          729 g          618 t
ORIGIN      
        1 cgagggacct tgcggaaaca tcaagtctgc gcagactgga aacggtaaca agcgagtccg
       61 ctatttgcga aagctgacct ctcgcagtag aaatatcgag tccacgcttt gactaaaatc
      121 actggtgcga ctgtcagacg atagctataa gaccaccgca gttcattctg ggtggtcttt
      181 tagttttgct ttcaagtttt ggtgcagcat ctctcagttc aaaccggaaa agaattccag
      241 aatgtaagat gccaggatgg gctgaaagct ttcgcggcag aatccgggcg gatcttgagc
      301 tggtttgaag agcgcgccgt ctgcgctgtg ttgcattatc tttggaaaag ggctcaggaa
      361 ggaaaagtga ccggcgtttt caatttcgcg gaagatgacc cggctcggtt ccgggacccc
      421 ttccaggacc aactttgcat ggtaggcggt gggtgtgatt tcgtcctttt ctcccaccat
      481 caggagaatt gggcaattga cggcattgag ggacccttgc ggtctgaacc agaccagggc
      541 aggggccatg agcacaagcg ctttgatgcg aggatcgctt tcgatggaag tgagagtgcg
      601 gcttctttcc aggggctcgt ttctgctgaa agccgtgggt acgccccccg ctgctgccag
      661 agctgtgtag gcccccatgg agtgaccgat tacggcgatc ttgtccgtcc gaatcagggg
      721 cgagaagcgc tcatcttcgc taaaccaatc tagcgccgtg ctcaagtgac cggggcgccg
      781 ctccaggttt tcaactgtgc cgtcccggct attgtcgttg cgattgttac ccggatgctc
      841 cggcataccg acgatgaagc cgctggcagc cagggttctg gctagctgcc ggtaaaccag
      901 atgagagccg ccgctgccgt gggagatcag gacaaggggg aagtttcccg ccaggggtgc
      961 ggcttccacc gccagagaga tgttataggg accgagcctg gtaggtgctt cttgttccgt
     1021 ggtgggatac ataatcacca taggaaaaga ctgaccggtt tccttctcct tcaggtttgt
     1081 tgtgcaatag cctgtgtttc tatccacttt tcgcttaact ccgttaatgg gcgggattat
     1141 ctcacatccg cctcgacttc cggctgctat tgccctttcg gcttctcctc aagtatcggg
     1201 tttgtcttcg atcttgatat ttgactgcca gttcatgagg accaaccttt tacgcggtat
     1261 attggcagca agattgacga gccggccaga atcgggcgga ccagtcggca gcgagccgcg
     1321 aggcaggagt tcaggctgta agccatgccc agtgtactgc aatccctatt gcaaagagtc
     1381 ttgagcctga ttatgccggc tcccagtctc ttgatagaaa caacttgcaa gaagctcaag
     1441 caagaagccc tcaagctagg ttccgacgct cgctcagccc ttgagggggg agcccagagc
     1501 gctgctttcg acttttcagg cgcctgggat ttgttctttg agcttttcga tactgccagg
     1561 cttttagcct tgaagtgccg catctggact gccctgaaag agccccaggt gacggcgcct
     1621 ttgacggtga atgccatgga atggggtgcc tgcctggtgc aactggccgg gcagttccgc
     1681 agcctgcaga gggagcgtag cggccttgat ggcgagacac tcatagccag gcagaatgcc
     1741 ctgatcgaga ggcgggtgca ggctaataat ttgaaggagc gcttgagcat tttggagaga
     1801 caggcgctcc ttaccgtcag gcagaaaatc caaactgatc tggaaaaagc cagggaagcc
     1861 ggtaacggtt ctcacaagaa aatcgaaaaa cgtatcacgg ctctgctttc cttgctcaat
     1921 tgccatcgct atgccctttt gagtgagaag tcgcactgga gtcggcgcaa taatgagtct
     1981 tccgatctgg gcgaaagtga attcgctgaa attgcagcta tgcgcgaggc ctctgtgcgc
     2041 gaggtgctca ttcatttgaa tgcctggaaa gatctatatg caggtctttt aagatagtat
     2101 tgggctttgg aattccgctt gccgcccttg gtttcaatat caaaagaggg gataagggta
     2161 ctaatgaggt gcaatataag atgcatcgtg aacgtagccg caataaagaa gtgcggatct
     2221 atttgtgccc tggctctctg ccttgaagtt tttgcttttg ccgctctcgg cgtgcctgtg
     2281 gctgccaaca atgaaactga tgcgctttac ggcatggctt cgcgcgaaat gtgcaaaggt
     2341 ttggccggca atcacaaagc tttggaacac ttgaacaagg ctcttgcaat tgatccgcgc
     2401 agtgccaaag gttggtgttt gaaagcgcat attctcggca ccatggaaga gagtgaactg
     2461 gcgctgccat gcatcgaaaa ggctctcaag cttgagccga aaaactttgt gctctggatg
     2521 gaaaaagcag agatctattg
//
LOCUS       DAOQNZ010000529         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23807_length_2532_cov_4.189746, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000529 DAOQNZ010000000
VERSION     DAOQNZ010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23807_length_2532_cov_4.189746"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PKW73_10080"
     CDS             complement(<1..1100)
                     /locus_tag="PKW73_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73677.1"
                     /translation="MALNLGGCAPSDFAVKQKVSTVLDVPVTQPDLKHLLAVTSKMQI
                     VASGQFAPGNYWSHKKQLSFAPDTSFQVNLEMPVENPEILSTRNATGFLATSKEFAVN
                     AVPVPKNIQLVEGKLTGEVEYGKWLGAFLVNLLQLGNNDGGLRSTIASMKIESARLEL
                     KPGAKLVFGQKSLIVGENSSITLNNLDIDHNLNYRGEMVFDLNFDKGCDWLGKRVDTR
                     FNGGFARLYLIVEGRDKTLTLSLNKEHPTVLDSRTVDIKLEDCRFNFGHEKLSSSISE
                     SAAIDVTDLKWSRISAVEEPTLELKSTMTLVATAADINTSSHQTKLTLPNAVNTVLDI
                     DTKGKESDTHFATMGDAVARTIAITLNKSHTSL"
     gene            complement(1222..2145)
                     /locus_tag="PKW73_10085"
     CDS             complement(1222..2145)
                     /locus_tag="PKW73_10085"
                     /inference="COORDINATES: protein motif:HMM:NF037206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-4TM family putative pore-forming effector"
                     /protein_id="HNA73678.1"
                     /translation="MTNSICRNHQKYKQYLSAYRLAYARAKSVRKLEIMLSFAPVIAC
                     LALYQLGPYISGGSREFPANLLNLCALAGVIMLLVCDLKLEPLRAMHRRAANVDLEAY
                     DTAVLEFPWHAAVTGERPSQSALDAEARGYDNALASAHPPELSLEDSWYPPLIETVAI
                     EPARLYCLRQNCSFEIMYRQKYMQLFLFPMLIITSLLIAVQIFLFGGSLPNTCCTLIT
                     ASPIFRMLLLGLTVQNRNCRLLQELKANIDEALKKQDTSAETARAFQDRLFLTRDTQP
                     LISIAGFKHIKLKYLPVLKAETEALLRSAGH"
     gene            complement(2166..>2532)
                     /locus_tag="PKW73_10090"
     CDS             complement(2166..>2532)
                     /locus_tag="PKW73_10090"
                     /inference="COORDINATES: protein motif:HMM:NF021584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HNA73679.1"
                     /translation="PEGTTYAAAEDMELKKARVEALDHFDEFATAFKNKQEGEKFAVK
                     AAFKDGDQEVHKWLIVDQMENLDIVGHFASGDEKGNLKAGQQATAKATFIDDWSYVDK
                     NGGEHGGYGLAVLRKRQAK"
BASE COUNT          521 a          606 c          722 g          683 t
ORIGIN      
        1 agactggtgt ggcttttgtt cagtgtaatg gctatggtgc gagctaccgc atcacccata
       61 gtggcaaagt gcgtgtcgct ttccttgccc ttcgtatcta tgtccagcac ggtattgacg
      121 gcgttcggaa gagtcaactt ggtctgatgg gaggaagtgt ttatgtcggc agcggtggcc
      181 accaaggtca tggtgctctt cagttcgagg gtgggttctt ccactgccga aatacgcgac
      241 catttgagat cggttacatc gatggccgcg ctttcgctta ttgatgatga cagtttctca
      301 tgaccgaaat tgaaccggca atcttccagt tttatgtcga cagtgcgact gtctaaaacg
      361 gtcgggtgtt ctttgttgag agaaagagtg agagttttgt cgcgcccttc cactatcaag
      421 tagagtctgg caaaaccccc attgaagcgg gtatctactc ttttgcccag ccagtcgcaa
      481 cccttgtcga aattcagatc gaagaccatt tcccctctat agttgaggtt atggtcaata
      541 tccaggttgt tcaatgtgat gctggaattt tctcccacaa tcaggctttt ttgaccaaag
      601 acaagctttg cgccgggctt aagctccagt cgggcgcttt caattttcat ggaagcgatg
      661 gtgctgcgca gaccgccgtc gttgtttccc agttgcagta ggttgaccag gaaggcgccg
      721 agccatttcc cgtattccac ttcgccggtg agcttgcctt ccaccagttg gatattcttc
      781 ggtaccggca cggcattgac ggcaaactct ttagatgtgg ctagaaagcc ggtggcattt
      841 ctggttgata aaatttccgg attttccaca ggcatttcca gattgacctg gaagcttgta
      901 tcgggggcga aggaaagctg tttcttgtga gaccagtaat tgccaggggc gaattgaccg
      961 gaggcaacaa tttgcatttt ggatgtgacg gctagaagat gcttcaggtc aggctgtgtt
     1021 accggcacat cgagtacggt ggagactttc tgtttgacag cgaagtcgga aggggcgcaa
     1081 cctcccagat ttaatgccat cgaagcactg agagttacac aaagcagaga tgagaacagc
     1141 ctgactgttc ttcctctagg cgctggctct ctttcgtgcg gctgtttcat ggggtttgaa
     1201 tatgtacttc ctctgtcttt ctcaatgtcc ggcagacctc agcagggctt ctgtttcggc
     1261 tttcaataca ggcaagtact tgagctttat atgtttgaaa ccagcaatgc ttatgagggg
     1321 ctgcgtgtca cgagtgagga agagtctgtc ctgaaaggcg cgggccgttt ccgccgaggt
     1381 gtcttgtttc ttgagggcct catcgatgtt ggcttttagt tcctggagaa gccggcagtt
     1441 gcggttctgc acggtcagac cgagcaggag catgcggaaa ataggtgaag cagtgatgag
     1501 ggtgcagcag gtgtttggca agctgccgcc aaagagaaaa atctgcaccg ctatcaaaag
     1561 gcttgtgata atcagcatgg gaaaaaggaa tagctgcatg tacttttggc gatacatgat
     1621 ctcgaaactg cagttttgtc tgaggcagta aagtctggcc ggttcaatgg ctacagtttc
     1681 gatcagaggc ggataccagc tatcttccag ggaaagctca ggtggatggg cgctggccag
     1741 ggcgttgtcg tagccgcggg cttccgcatc gagcgctgac tggctgggcc tttctccggt
     1801 gacggcggca tgccagggga attccagaac ggcggtgtcg taggcttcca gatcgacatt
     1861 ggcggcccgc ctgtgcatag cccgcagtgg ctcaagcttc aagtcgcaga cgagcagcat
     1921 gattacgccg gccagcgcgc agaggttgag caggttggcg ggaaattccc ttgaaccgcc
     1981 ggaaatgtag ggaccaagct ggtagagggc caggcaggcg atgaccgggg cgaaggacag
     2041 cattatctcc aacttcctga cacttttggc gcgcgcatag gcgagccggt aggctgaaag
     2101 atattgcttg tatttttgat gattgcgaca gatggagttg gtcatagtcg tttggactga
     2161 ctcctctatt ttgcctgtct ttttctcagg actgccagcc cgtagccgcc gtgctcgccg
     2221 ccgttcttgt ctacgtagct ccagtcgtcg ataaaggttg ctttagctgt ggcttgctgt
     2281 ccggctttca gattgccttt ctcgtctccg gaggcgaagt gcccgacgat gtccaggttc
     2341 tccatttgat ctacgatcag ccatttatgt acttcctggt cgccgtcctt gaaggcggct
     2401 ttgactgcaa atttttcgcc ttcctgttta ttcttaaagg ccgtggcaaa ctcgtcaaag
     2461 tggtctaagg cttccactct cgctttcttc agttccatgt cttcggctgc ggcataggtg
     2521 gtgccttccg gc
//
LOCUS       DAOQNZ010000530         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23831_length_2531_cov_2.707997, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000530 DAOQNZ010000000
VERSION     DAOQNZ010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23831_length_2531_cov_2.707997"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..796)
                     /locus_tag="PKW73_10095"
     CDS             complement(110..796)
                     /locus_tag="PKW73_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73680.1"
                     /translation="MSCERNQKCFQPQQDPLAAEGRALAELLDNRQPEQVANFLRQDS
                     YNMSPGAFNKVINEARRNDRKGVGADLYIDNDGLVVIDTRNRNGDQLVVATREYDQRM
                     GRVPQQHDDRQVDRRGQPIYRPVDPRDQHGQRRDAVTDTVVDGVVGAAVGAAIHGKKG
                     ALAGGAGAVAGKMVDQVGGPNNRDQVTDTVVKGVVGAAVGAAIDGKEGAKAGAAGVLV
                     GKGLDWLRNK"
     gene            complement(1190..2182)
                     /locus_tag="PKW73_10100"
     CDS             complement(1190..2182)
                     /locus_tag="PKW73_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73681.1"
                     /translation="MTRELRSPRTSVASIASLASVLILASGLALSQTTGAEAQGNAAQ
                     TLKLIQTGKFKQAYPSIKAMAQAGPREMSLQYYLGICAQGIGDKPTAELALTRVIVGT
                     EANSPYNVNAMLSLQKLQPTLRPYSCTRAGKSQRWAPQSMPLKMYITDGQGVNPNLSG
                     RDLAATEVTQVVNHVRTGGLHPQAGYKPSFKRAIADGIQVWDWAIKAKLVNFALVGSP
                     NNADVIVLYCQRTQEGFGGITVCPPEKGQPCIVQISLEQAAALPEAEAFRLIKQRAAV
                     QIGHVMGLARSSSPQDLMYESSQNPTAVGAAAPSQNDLASLRALYSMPCDIFFK"
BASE COUNT          525 a          627 c          729 g          650 t
ORIGIN      
        1 tgcttcatgc tctcgggaac atggaacaag gggacttttg ctttgccagt ctgagttgaa
       61 ctaccactgc accttaagag atgcttgcca gccgtgaacc ttcgcgcttc tatttgttgc
      121 gcagccagtc cagacctttg ccgacgagga caccggccgc accggccttg gcgccttcct
      181 tgccgtcgat ggcggcaccg actgccgcac ccactacacc tttgactacg gtgtcggtga
      241 cctgatcgcg gttattgggt ccgcctacct ggtctaccat tttgcctgcc acggcaccgg
      301 ctccgccagc cagagcacct ttcttaccat ggatggcggc accaactgcc gcacccacta
      361 caccgtctac aaccgtatcg gtgacggcgt cgcggcgttg accgtgctga tctctgggat
      421 cgaccggtct gtagatgggc tgtccgcgac ggtctacctg tctgtcgtca tgttgttggg
      481 ggacacgtcc cattctctgg tcgtattcac gggtggccac caccagttga tcgccgtttc
      541 tatttctggt gtcgattacc accagtccgt cattgtcgat gtaaaggtcg gcgccgacgc
      601 cttttctatc attgcgacga gcttcattga tgactttatt gaaagcgccg ggagacatat
      661 tgtaggagtc ttgtctcagg aaattagcaa cttgctcagg ttgtctgttg tcgagcagtt
      721 cagccagggc tctgccttca gcggcgagag gatcttgttg gggttgaaaa cacttctggt
      781 ttctttcgca tgacatagca aagcactcct gaacatggtt tgggaggggg atatatttca
      841 gactgcactc ggggggaggt gtgtattagc aaaccataac tatcgagtgg ttgcaatgag
      901 ggaaactacg tagttgctgt ttttaacgtt tatggcactt ttgtgacttg gcgtaaactg
      961 cgataaacac tgggttttgc tcctgcaatc ggacagcggc gtggaagaaa tcttttcttc
     1021 agcgccaggg acttgcaacc aaaagtggta tcaaactatg ccacgagaat gtcacaaacc
     1081 tgcgaaaagc agcactgcag gctgtgtcac aaaagtgtca caaactgcct gcaatttgag
     1141 gaaatgacat tctcaattat tggtcttgtc gtcgacccgc gagtctctct tatttgaaga
     1201 agatatcgca gggcatggaa tagagtgcgc gcagggaagc caggtcattc tgactggggg
     1261 cggcggctcc gacggcggtc ggattttggc ttgattcata cattagatct tgtggagaac
     1321 tggagcgggc cagtcccatc acatgtccta tttgcacagc cgctctttgc ttaatcagtc
     1381 tgaaagcttc cgcttccggt aacgcggcgg cctgttccag ggagatctgt actatacaag
     1441 gttgtccttt ttccggcgga cagaccgtga tgccgccgaa tccttcctgg gttctctggc
     1501 agtagagcac gattacgtcg gcattgttgg ggctgcccac cagggcgaaa ttgaccagct
     1561 tggctttgat tgcccagtcc cagacctgga tgccgtctgc tatagccctc ttgaaagagg
     1621 gtttgtagcc ggcctgggga tgcaaaccgc cggtacgcac gtgatttacc acttgagtaa
     1681 cttccgtggc ggcaaggtcg cgaccactga gattgggatt caccccttgc ccgtcggtaa
     1741 tgtacatctt caggggcatt gattgcggag cccagcgctg gctcttaccg gcccttgtgc
     1801 aggaataggg gcgcagggtt ggctgtaact tttgcaagct cagcatggcg tttacgttgt
     1861 agggcgagtt ggcttcggtg cccacgatta ccctggtgag ggcaagctcg gcagtgggct
     1921 tatctccaat accctgggca cagattccca gataatactg gagagacatt tccctgggac
     1981 cggcctgggc catggcttta attgacggat aagcttgttt gaacttaccg gtctgaataa
     2041 gtttaagggt ctgcgccgca ttgccctgcg cttccgctcc ggtagtctgg ctgagagcaa
     2101 gacccgatgc cagtataagg acagaggcca gggaggcaat cgaggctact gaggttcggg
     2161 gggagcgcag ttctcttgtc aatttgaagg gacctgattt tcaagcggtt atgggaagca
     2221 gagatttata tataaagttt agcagttccc gccggcgggt gcttttgcca gaggcattgc
     2281 ggtataaggc ttttggggag tttgcctgtt ttcctgtccg gggtgatgac tacgctaata
     2341 aggatgtaaa atcaggggaa gcgtaccatg tactggttcg agtaccggtc tgtcggcaca
     2401 atgaggattt taatggctgc ttcacaagtc ttagtggctg aggataacga agtcaataga
     2461 gcggttctgg ttaagcaact ggaaggtctt ggttgcagtg ccgtagcggt agaaagcggc
     2521 gaagcagcgg t
//
LOCUS       DAOQNZ010000531         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23841_length_2530_cov_4.216162, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000531 DAOQNZ010000000
VERSION     DAOQNZ010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23841_length_2530_cov_4.216162"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="PKW73_10105"
     CDS             <1..1556
                     /locus_tag="PKW73_10105"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73682.1"
                     /translation="WPLVQGTVTASSLKKVEKKWGESYEAEIRYEYQVNGKTYSGDRV
                     QFGSSAFQDAAATVAKYPLNAKIEVRYEKANPAASALEPGFNQLPVLASLLGAILIIC
                     LGFKEFAHGESRKPSLAVSTNSTANKASASSTANVGRAVPTITIAALVAIALAIPWAA
                     DHLVPKGINAEWLTFAILFGGGLGLVIFFSTLPALIAHFVRTRQFQIAGALADFNGFV
                     FSTFFPVSVEAAFAAALQSDIAREQLAFAKANRLAEKAIAVGTEWLNLTTRFVQEDDN
                     LDLKKIAMKSNLQFQEAHCREIVSLCHHDIGETAFLLGRYDEAMNRARIAADLAQAQM
                     STDKLRNGSSIKPGALALANALTLKGRLANRLDSHEEARKDLEKALALRNELQTQFSE
                     KRAELLAHLASCYSLQGESKKAQDAIAAGLELVANANGPQFQLATATLLQSQGQLLMR
                     EGKLKEAEVNLKECLRLRTKLHPTNHPQIAETIMVLADLLTEQGQSVKALDLNNKAQK
                     LLKDCQVYA"
     gene            complement(1571..>2530)
                     /gene="panE"
                     /locus_tag="PKW73_10110"
     CDS             complement(1571..>2530)
                     /gene="panE"
                     /locus_tag="PKW73_10110"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNA73683.1"
                     /translation="EFRGRITKPMRILVLGAGATGGYFGGRMLEAGADVTFLVRPQRA
                     ERLRREGLVVESHYGNLHLRDLALVEKVQEPFDLVILSCKAYDLEGAMEAVAPAVGAE
                     SMVLPLLNGMQHLSVLQKRFAEENILGGCCVISSELSEDGRIVHLNKNHSLRYGELSG
                     VMSERMQELEEVIAPCNFTSQASFSIVQDMWEKWVLIASLAALTTLMRATVGDIARAP
                     FGKEIAEQLASECLAILKAHDFEPSSAFVEATYSRLVDPASTLTASMLRDMERGNRVE
                     GKQILGDFIHMAKAKELSVPVLQAAYCHVSAYELRRGLCDTAS"
BASE COUNT          679 a          714 c          600 g          537 t
ORIGIN      
        1 actggccgct ggtgcaagga acggtaactg ccagttccct caaaaaagta gagaagaagt
       61 ggggtgaatc ttatgaagcc gaaattcgtt atgaatacca ggtgaacggc aaaacctaca
      121 gcggcgaccg ggttcagttc ggttcatcgg ccttccagga cgcagccgcc actgtggcta
      181 aatatcccct caacgccaaa atagaggttc gctatgaaaa agccaacccg gctgcatctg
      241 cactagaacc gggattcaat caactaccgg tgctggcgtc gctcctcggc gcaattctaa
      301 taatttgcct tggcttcaaa gaattcgccc atggcgaaag tcggaaaccc agtctggcag
      361 tctcaaccaa ttcaacagca aacaaagcct ctgcaagttc tactgccaat gtaggtcgtg
      421 ccgtccctac tattacaata gcggcactgg tggcaatcgc cctggcaatc ccctgggccg
      481 ctgatcatct tgtgccgaaa ggcataaatg ctgagtggct gacctttgca attttgttcg
      541 gcggaggact gggtctcgtc atcttcttct cgaccctacc ggctttaatt gctcatttcg
      601 tgcgcaccag acagtttcaa attgcaggcg ccctggccga tttcaacggc ttcgtattca
      661 gcaccttctt cccggtttcc gtcgaggcag ccttcgccgc cgcacttcaa tcggacatag
      721 ccagagaaca actggctttt gccaaagcca atcgtctggc tgaaaaggct attgctgtgg
      781 gtactgaatg gctgaatcta accaccaggt ttgtgcaaga agatgacaat cttgacctaa
      841 agaaaattgc aatgaaaagc aatctccaat ttcaagaggc tcattgccgc gaaatcgtct
      901 ccctttgcca ccatgatata ggagaaaccg cctttcttct tggccgctat gatgaagcca
      961 tgaaccgggc ccgtattgca gcagacctgg cgcaagcaca gatgagcaca gacaagttga
     1021 ggaatggaag cagcatcaaa ccgggtgcac tggccttagc caatgctctc actctcaaag
     1081 gtcgcctcgc caataggctc gactcacacg aagaggctag aaaggatctg gagaaagcct
     1141 tagctctgag aaatgagctg cagacgcagt ttagcgaaaa acgggcggaa ctactggcgc
     1201 acctggcctc ctgctattca ctgcaaggtg aatcaaagaa agctcaagat gccattgctg
     1261 ccgggctgga actagtggcc aatgccaacg gaccacaatt ccaactggcc accgccacct
     1321 tgcttcagag ccagggtcag ttactgatgc gcgaaggcaa gctcaaagaa gcagaggtga
     1381 acctgaagga gtgcctgcga ctaagaacca aactacaccc caccaatcat cctcagattg
     1441 cagaaaccat catggtctta gcagacttac tgactgagca agggcagagc gtgaaggcat
     1501 tggacttaaa caataaggcg cagaaacttc tcaaagattg ccaggtatac gcctaggtac
     1561 gaagaaagac tcaagaggca gtgtcacaaa gtccgcgcct cagttcataa gcgcttacat
     1621 ggcagtaggc agcctgcaac acaggcacag acagttcttt agcctttgcc atatggataa
     1681 agtctccgag aatttgcttt ccttccactc tattgccacg ctccatatcg cgaagcatag
     1741 aggccgtgag agtagaagca ggatcgacca gacgcgaata agtggcttcc acaaaagccg
     1801 acgacggctc aaaatcgtgc gctttcaaaa tggccagaca ttcagaggcc agttgctccg
     1861 ctatctcttt tccaaaggga gcgcgggcga tatcaccgac cgtagcccgc atcaaggttg
     1921 taagcgccgc cagactggca atcaagaccc acttttccca catatcctga acaatgctaa
     1981 agctagcttg ggaagtgaaa ttacaggggg cgatcacctc ctccaactct tgcatgcgct
     2041 cactcatgac accggataat tcaccatagc gcaggctgtg gttcttgttg agatggacaa
     2101 ttcgcccatc ttcagacagt tccgacgata tgacgcaaca gcctccgaga atattttctt
     2161 cggcaaagcg cttctgcaaa accgacaggt gctgcatacc gttgagcaga ggcaagacca
     2221 tagattcggc tcccacagcc ggtgccactg cctccatggc accctctaaa tcgtaagcct
     2281 tgcaggacag tatgactaaa tcaaaaggct cttgcacctt ctctactaaa gcaagatcgc
     2341 gaagatgcaa attgccgtaa tgactttcca caactagccc ctcccgcctc agccgctcag
     2401 ctcgctgcgg ccgcaccagg aaggtcacat cggccccggc ttccagcatt ctgccgccga
     2461 aatatcctcc ggtggcaccg gctcccaata ctaaaatgcg catcggcttc gtaattctcc
     2521 ccctaaattc
//
LOCUS       DAOQNZ010000532         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23865_length_2529_cov_3.650768, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000532 DAOQNZ010000000
VERSION     DAOQNZ010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23865_length_2529_cov_3.650768"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKW73_10115"
     CDS             complement(<1..452)
                     /locus_tag="PKW73_10115"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNA73684.1"
                     /translation="MTEAMKVRVEGVVQGVGFRPFVYTLAKSHNLSGWVLNDEQGVEI
                     FLEGARGDLDAFLASLSERAPQAARLDDVKFETVAVQGFSQFEIVESRAGDRITTRIS
                     PDLSVCSDCLADLNNPADFHYEYPYVNCTNCGPRFSIIRALPYDRSATT"
     gene            complement(465..1499)
                     /gene="hypE"
                     /locus_tag="PKW73_10120"
     CDS             complement(465..1499)
                     /gene="hypE"
                     /locus_tag="PKW73_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNA73685.1"
                     /translation="MVTEPFRDTRIELSHGSGGLASRRLLEGLIVPHLASSQFNAGAL
                     TDAAVLPIESGHLAFTADSFVVKPLRFAGGSIGSLAVHGTVNDLAVSGARPLALLSTF
                     ILEAGLPAVVLEAELAAMAAACSAAGVNIVGGDTKVVEHGKADGMYVTTCGIGMLEAN
                     VNLSPARVQVGDKVLLSGPIGDHGITVLLARGELDIEAELASDSRSVWPMVQALLAVC
                     AAEVRWMRDPTRGGVASSLNELAQDAELCVALREKDIPVRDCVRGACEVLGLDVLHVA
                     NEGQFLAVVAASAAEQALSAVRAVPGGEAAALIGEVESGPSGRLRVFTEHGSSRVVEM
                     LVGEQLPRIC"
     gene            complement(1489..>2529)
                     /gene="hypD"
                     /locus_tag="PKW73_10125"
     CDS             complement(1489..>2529)
                     /gene="hypD"
                     /locus_tag="PKW73_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNA73686.1"
                     /translation="THSIYRFGLEQLLPPAIELIHGPGCPVCILPMERVDEGMAIAAD
                     KRVILTAYGDMMRVPGHQGSALQLKARGLDIRMVYSPLDALKLAQNNPEREVVFFAIG
                     FETTAPATALTVLKARELGLTNFTAFVNHVTVIPPMRAVLDDPKLQLDGFIGPGHVAT
                     VIGANSYEEIVRDYKKPVIVSGFEPLDIMQSILMLLRQLLAGEARLENQYSRVVPFAA
                     NPVASQVLSEVFELRESFEWRGLGFIANSAVKLRAAFSQYDAEVRFADVLSAYRNSSE
                     HASELSTVAHEAPCGDVLKGLMKPKACPFFGKECSPENPVGALMVSSEGACAAYYNYA
                     RDKQTGMLTHGN"
BASE COUNT          656 a          779 c          616 g          478 t
ORIGIN      
        1 gtagtggccg accgatcgta aggcagcgcc ctgataatgg aaaaccgggg accacaattg
       61 gtacagttca cataagggta ttcgtagtga aaatcagccg gattgttcaa atcggcaaga
      121 cagtcggaac aaacagacag atctggtgaa atgcgagtgg taatacggtc accggcacgg
      181 ctttcgacaa tctcgaactg actgaagccc tgaactgcca cagtctcaaa cttgacatca
      241 tcgagccgag ccgcctgggg agcccgctcc gacagggaag ccaggaaagc atccagatca
      301 ccccttgctc cttccagaaa aatctctacg ccttgctcat cgttcaagac ccatccactc
      361 aagttatggc tcttggccag cgtgtacacg aagggacgaa agccgacgcc ctgaacaacg
      421 ccctctacac gcactttcat agcttcagtc atgcctgccg acctctagca aatgcgcggt
      481 agctgctctc ccaccagcat ctccaccact cggctggaac catgctcggt gaacacccgc
      541 agtcgaccgg acggaccaga ctccacttcc ccaatcaaag ccgccgcttc ccctccggga
      601 acggcgcgca ccgccgaaag cgcttgttca gcggccgaag ccgccacaac cgccaggaac
      661 tgcccttcat ttgccacgtg cagcacatcc agaccaagca cttcgcaagc cccgcgcaca
      721 cagtcgcgta ccggaatatc tttttctctc agagccacac acagttcggc atcctgggcc
      781 agctcattca aagacgatgc tacgccaccg cgggtgggat cacgcatcca gcgtacttcg
      841 gcggcacaaa cggcaaggag cgcctgcacc ataggccaga cgctgcggct gtcggaggcc
      901 aattccgctt caatatccag ctcaccgcga gccaaaagaa cggtgatacc atgatcaccg
      961 atgggcccac tcaaaagtac cttgtcaccg acctgaactc tcgcgggcga gaggttgacg
     1021 tttgcttcca gcatgccgat accgcaagtg gtgacataca taccatcggc tttaccatgc
     1081 tctactactt tcgtatcgcc gccaacgatg ttaacacccg ccgccgaaca ggccgcggcc
     1141 atggccgcca attccgcttc caacaccacc gccggtagcc cggcctctag aatgaaagta
     1201 ctgagaagag ccagcggccg cgcaccagag acggccagat cattgaccgt gccgtgcacg
     1261 gccagagagc cgatactgcc gccggcaaag cgcaggggct tgaccacgaa actatcggca
     1321 gtaaaagcta gatgaccact ttctatgggc aaaactgctg catcggtcag agccccggca
     1381 ttgaactgag aactggccag atggggaact ataagaccct ccagtaagcg gcgactggcg
     1441 agaccgccgg agccatgtga cagctcgatg cgcgtatctc gaaagggctc agttaccatg
     1501 ggtaagcatt cccgtctgct tgtctcgtgc ataattgtag taggcggcgc aggctccttc
     1561 cgaagaaacc atcaaggctc ccacaggatt ttcaggactg cattccttgc caaagaacgg
     1621 gcacgcctta ggtttcatca agcctttcaa gacatcaccg cagggcgctt catgggcaac
     1681 agtcgacagt tcggaagcat gctcagaact gttgcgataa gcagaaagaa catcggcgaa
     1741 tcgcacttcc gcatcatact gactgaaagc tgccctcagc ttcacggccg agttggcaat
     1801 aaaaccaaga cctctccact caaaactctc tctcagttca aagacttccg acaatacctg
     1861 gctggccaca ggattagccg cgaagggcac cacccgactg tattgattct ccagcctggc
     1921 ctcaccagcc agcaactgac gcaaaagcat caatattgac tgcatgatat cgaggggctc
     1981 gaaccccgat acgataacag gctttttgta atcacgcaca atttcttcgt aactattggc
     2041 accaatgaca gtggctacat gacccggtcc gataaagccg tccaactgca gcttaggatc
     2101 atcgagaacc gctcgcattg gcggaatgac agtaacatga tttacaaagg cggtaaagtt
     2161 tgtaagaccc aactcgcgtg cttttaatac agtcaaagcc gtagccggtg ccgtagtttc
     2221 aaaaccaatg gcaaaaaaca caacttctct ttccggattg ttctgagcca gcttaagcgc
     2281 atccagcgga gaatagacca tgcgaatatc aagcccccgt gctttcaact gcaaagcact
     2341 accttgatgc cccggcaccc tcatcatgtc accataggcg gtgagaatca cgcgcttatc
     2401 ggcggctatg gccattcctt catcaacccg ctccatgggc aggatgcaaa ccggacagcc
     2461 gggcccgtga atcagttcga tggcaggcgg caacagttgc tcaagaccga agcgataaat
     2521 ggaatgagt
//
LOCUS       DAOQNZ010000533         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23901_length_2527_cov_3.648867, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000533 DAOQNZ010000000
VERSION     DAOQNZ010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23901_length_2527_cov_3.648867"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..790
                     /locus_tag="PKW73_10130"
     CDS             386..790
                     /locus_tag="PKW73_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73687.1"
                     /translation="MEEHINVENTKPRDNLENETTDKPKGSMKEECWQSIKSHPLETG
                     LAAASMAALAIFSRGKVLPRLLIEEAEVTAKAPAPLKLVHSAPNVLTANTKTAFSLRL
                     ASKDGQLASGFQPTLNLDRTTEKAKPVSKLAD"
     gene            955..1191
                     /locus_tag="PKW73_10135"
     CDS             955..1191
                     /locus_tag="PKW73_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73688.1"
                     /translation="MGLDERGEFFLNPLTKISDLHLETFEVNPQARSLGEMTGVHKPR
                     STYLEDSAYLLIRPEAQRSQNFLSIFAHRNYLGH"
     gene            1452..>2527
                     /locus_tag="PKW73_10140"
     CDS             1452..>2527
                     /locus_tag="PKW73_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73689.1"
                     /translation="MDSPEIKKEPENKTGKRSEACDKLANEALENESGARIQKETNQK
                     SNSACQYLPALELSGLETEKDEKPFEQQRKEYQERLNFIENTRLVEGNHERSLKLGIS
                     NSLKEIESSTFMDGLLGNKERRETELKHRQEELVDVERGRNSAQKAMNAVSPDIARVE
                     ELDKREKQERQHGDIEKAEQTAKEAQAQMQKVMEATEGVAYIKRGTLENSGTNRQAEL
                     VEVENFKENLKTTKVIADTVRDGAVAGAAMAVSGGTLAPVALQYGVGLATVSAIGAGT
                     AAGTGVGAVSRLTEATSDIYVNNKDPKAALTEAAINTMQDAKTALITSTSTLAGLGIS
                     SKLSTNLTTQSAALKSISGGAIAGG"
BASE COUNT          804 a          675 c          574 g          474 t
ORIGIN      
        1 cacttgctac agcgcccttc tcaacgcctt tgaaaaggga attcacatct atactattca
       61 gttcccgctc ggctgccgcg ggtgacgaaa cttccttatg tactgcaatt tcaggcatgg
      121 ctctggcctt ccaatttact aatgaacgat ggggcaccgc acgcacttca tgctagaggt
      181 ccgccccgca atgcaaacgt gtcccaagtc caaatctaac catgacaaaa gtcacagcca
      241 ggcgtcccga aaagcaagcc gagcagactg tgacttccga cacaagccaa aaacactgtc
      301 accaactaaa gtagcaaaca gaacgtttct accctgctgc gccaaaaagg cgactgtatt
      361 tcgaatatca ataaaggaaa aggaattgga agaacatata aatgtagaaa atacaaaacc
      421 acgtgacaat ttagaaaacg agacgacgga caaaccaaaa gggagcatga aagaagaatg
      481 ctggcaaagc attaagtcac atccccttga aaccggtctg gcagcagcct ctatggcggc
      541 ccttgccata ttttctcgag gcaaagtcct accccgattg ctgattgagg aagccgaagt
      601 gacagcgaaa gcaccagcgc ctctaaaatt agtacattcg gcaccaaatg tactaaccgc
      661 aaacaccaaa accgctttct ccctaagact ggcaagcaaa gacggacagc tggcatccgg
      721 attccaacct acactcaact tagatcgaac tacagagaag gcaaagccgg tgagcaaact
      781 agccgactga cttcagaagc tgaatcaaga aaggctacac acagcctcaa tttattaaag
      841 ccgatctaca accgggggaa gccgctgtcg gcaaaggcat ctccagatga atacggcttc
      901 acacacaaat tggatccggt tgatgctaaa actacccccg atatctggct caggatgggt
      961 ctagatgaac gcggagaatt ctttctcaat ccactgacga aaatcagcga tctacactta
     1021 gaaaccttcg aggtaaatcc ccaggcaaga tcacttggtg aaatgaccgg tgtccacaaa
     1081 ccccgcagca cctatctgga agactccgcc tatctactca tacgtccgga agcacagcgc
     1141 tctcaaaact ttctttccat cttcgcccat cgaaactatc tcgggcacta acagcaacta
     1201 tcaataatga ggctgcattg cacgatgcaa ggacgtaaag catgcgcttc tgcatccctg
     1261 ttatgcctct gccataatcc tgacatagtc ctgccataga cacactctaa gctagtctag
     1321 cctcgatctc ataaacgccc cctcgaggcc attaccatcc tttccgcatc tatcaaagta
     1381 ttttccaagc cgcctgcaat tagacggcca atacttgcct ttctcatttt ctacgccacg
     1441 gtaaaccacc tatggacagc ccggaaataa aaaaggaacc tgagaacaag accggcaaac
     1501 gctccgaagc ctgtgacaaa cttgccaacg aggcactaga aaatgagagt ggtgcccgga
     1561 ttcaaaagga aacaaaccag aaatcgaaca gcgcctgcca atatcttccg gctctcgagc
     1621 taagcgggtt ggaaaccgaa aaagatgaaa agccctttga gcaacaacgc aaagaatatc
     1681 aggaacgact gaatttcatc gaaaacacca ggctggtaga aggaaaccac gagcgctcgt
     1741 tgaaactggg catcagcaat tccttaaagg aaatcgagtc atccactttt atggacggtc
     1801 tgcttggcaa taaggagcgc cgcgaaaccg aactaaagca tcggcaagaa gaacttgtcg
     1861 atgtggaaag aggaaggaac tctgcgcaga aggcaatgaa tgcggtcagc ccggacattg
     1921 ccagagtgga agaactagac aaaagagaaa agcaggaaag acaacacggt gacatagaaa
     1981 aagccgaaca aacagcgaaa gaagcacaag ctcaaatgca aaaggtcatg gaagccaccg
     2041 agggtgtcgc ctatatcaaa cggggaaccc tggaaaacag cggtacaaat cggcaagccg
     2101 aactagtcga agtagaaaac tttaaggaga atctaaaaac cacgaaagta attgccgata
     2161 cagtcaggga cggtgctgtt gctggcgcgg ctatggcagt cagcggcggc acactggcac
     2221 cggtggcact gcaatatgga gtgggtctgg caacagtttc ggcaatcgga gcgggaacgg
     2281 ccgccggcac aggcgtaggt gcggtaagcc gtctgacgga agccacttcc gacatctatg
     2341 tcaacaataa agacccgaag gcagccctga ccgaagccgc aataaatact atgcaagacg
     2401 cgaaaactgc gctcattacc tctacctcaa ctcttgcagg gctcggcata agcagtaaac
     2461 tttcaaccaa tcttactact cagtctgcag ctctcaagtc catctcgggt ggcgccatcg
     2521 ccggcgg
//
LOCUS       DAOQNZ010000534         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23916_length_2526_cov_4.189397, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000534 DAOQNZ010000000
VERSION     DAOQNZ010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23916_length_2526_cov_4.189397"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKW73_10145"
     CDS             complement(<1..680)
                     /locus_tag="PKW73_10145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA73690.1"
                     /translation="MAAAPEPTAVITGAASGIGRATALAYLASGCNVVAVDIDENGLK
                     KLSKEAAASMNSQVGLEILAADIADPVTARRTQALSKRAFGGIDYLFNNAGNEFIAPL
                     METEEADWDRVIDTNLKGTYLITRACLELMLEAGRGVITNNASDAGLRGIKLNAAYST
                     SKAGIIHLTRSLALDYTGKGIRSNCICPGCIRTPLCERFNAEVGARKGKSGEEVLQEF
                     VQENIPMA"
     gene            complement(680..964)
                     /locus_tag="PKW73_10150"
     CDS             complement(680..964)
                     /locus_tag="PKW73_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73691.1"
                     /translation="MSNNENMSEDQKWELLTRSEKLGEVLLKRGKLTLQQLEDLIKEQ
                     ERTESPLGELILAKGWMTRQELLSALDLQHKTDQAIIDSLTEMIQRNNEG"
     gene            complement(976..1938)
                     /gene="secF"
                     /locus_tag="PKW73_10155"
     CDS             complement(976..1938)
                     /gene="secF"
                     /locus_tag="PKW73_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNA73692.1"
                     /translation="MFSKVNVDIVKYRKIWLGISLLITIPGVLGIIACMSKYHAPLKP
                     GIDFTGGSILQYQFDKPTSLESVHKVLEDCGFHGSQVQQATISGQEAVVMRTKSIDTE
                     AQKAALDKKLTEELGTFKAISVDKVTATIGPELLSNGLIALLVTFGGMVAYISYRFKF
                     DYAACAIAALAHDIMVLVGIFALLGYFVGTEVDSLFISAVLTVIGFSVHDTIVVFDRI
                     RENAKYVGNKVIDPVSKVEYKRTFGDIANDSVNQTLARSIYTSLTVIITLLALYLLGG
                     VTTKDFVLAMLIGIISGTYSSIFNASCLLVWWREQKSTSKPRPA"
     gene            complement(1939..>2526)
                     /locus_tag="PKW73_10160"
     CDS             complement(1939..>2526)
                     /locus_tag="PKW73_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HNA73693.1"
                     /translation="EILEERSVGATLGQDSIQKSLVAGCVGIAAVMIFMVAIYGLPGL
                     IADLALILYTIATLAIFKTVPVTLTLAGIAGFILSIGMAVDANILIFERTKEELSRGR
                     NLYVAIENGFSRAMSSIFDSNVNSLIACAVLMVFGTSIVKGFAVTLAIGVAISMFTAI
                     SATRTMLHLIPKEWGLFGHRFDGKKVGQPASKEAV"
BASE COUNT          541 a          624 c          735 g          626 t
ORIGIN      
        1 ctagccatgg ggatattctc ttgcacgaat tcttgcaaca cttcttcgcc gcttttccct
       61 tttcttgctc ctacttcggc attgaatctc tcgcaaagcg gtgtgcggat gcagccaggg
      121 cagatgcaat tgctgcgtat gcctttacct gtatagtcga gggccaggga acgggtgagg
      181 tgaatgatgc cggccttgga tgtgctgtaa gcggcgttta gtttgatgcc gcgcagtccg
      241 gcatcggatg cgttattggt gataactcct cgcccggcct caagcatcag ttccaggcag
      301 gcccgggtga ttagataggt gcctttcaga ttggtgtcga ttactcggtc ccaatcagcc
      361 tcttcggttt ccatcaatgg ggcgatgaat tcgttgccgg cattgttgaa gagatagtcg
      421 atgccaccga aggctcgctt agaaagggcc tgggtgcgcc tggctgtgac cgggtcggct
      481 atgtccgcgg cgagaatctc caggccgacc tggctgttca tggaagctgc cgcttctttc
      541 gagagctttt tcagaccgtt ttcgtcaata tccacggcta ctacgttgca gcctgatgcc
      601 aggtaagcca gggcggtggc tcgacctata cctgaggcag cgccggtgat tactgccgtg
      661 ggctcagggg cggcagccat cagccttcgt tattgcgctg gatcatttcg gtaagggaat
      721 cgataatggc ctggtcggtc ttgtgttgta gatcgagggc cgagagcagc tcctgtctgg
      781 tcatccagcc tttggccaaa atgagttcgc ccaggggtga ttcggtgcgc tcttgctctt
      841 taatcaagtc ttcaagctgc tgcaacgtca atttaccgcg cttcaataag acctcaccaa
      901 gcttttccga gcgggtgaga agttcccatt tctggtcttc cgacatgttt tcgttgtttg
      961 acatttcgct atctctcagg ctggtctggg tttgcttgtt gatttttgct ctctccacca
     1021 gacgagcagg caactggcgt tgaagatgga ggagtaggtg ccggaaataa tgccgatgag
     1081 catggccagc acgaaatctt tggtggtgac gccgcccagc aggtagaggg cgagcaaggt
     1141 aatgataacg gtcaaagatg tgtaaattga tctggccagg gtctggttga cgctgtcatt
     1201 ggcaatatcg ccaaaagttc ttttgtactc gactttgctt accggatcaa ttactttatt
     1261 gcccacatat ttggcgttct cacgaatcct gtcgaagacc acaatcgtgt cgtgaacgga
     1321 gaagcctata accgtcagta cggcagatat gaacaagctg tctacttcgg tgcccacgaa
     1381 atagccgagc agggcgaaga tgcctaccag caccatgata tcgtgggcca gggcggcgat
     1441 ggcgcaggcg gcgtagtcga acttgaagcg ataactgata taggccacca tgccgccgaa
     1501 ggtaaccaga agtgcgataa gtccgttgga gagtagctcc ggtccgatgg tggcggtgac
     1561 tttgtctacc gagatagctt tgaatgtgcc caactcttcc gtcagctttt tatccagggc
     1621 tgctttctgg gcttccgtat caatagattt ggtgcgcatc accactgctt cctgtccgga
     1681 aatcgtggct tgctgcacct gggagccatg gaagccgcag tcttccagaa ctttatgaac
     1741 agattcgagg cttgtcggtt tatcgaactg gtattgcaga atggaaccgc cggtgaagtc
     1801 gatgcccggc ttgagcggtg catggtactt ggacatgcag gcgatgatgc ccagcactcc
     1861 cgggattgta atcaggagcg agatgcccaa ccagatcttt ctgtatttga ctatgtcgac
     1921 gttgactttc gaaaacactt aaacggcctc cttgctggct ggttgaccga cttttttccc
     1981 gtcgaagcga tgaccgaaga gaccccattc tttcggaatg aggtgcaaca tggtgcgcgt
     2041 ggcggaaatg gctgtaaaca ttgagatggc cacaccgatg gctagcgtaa ctgcaaagcc
     2101 tttcacgatg gaggtaccga agaccatcaa gacggcgcag gcgatcaagc tgttgacgtt
     2161 ggagtcaaag atggaagaca tggcgcggct gaaaccgttt tcgatggcta catagaggtt
     2221 gcgaccacgg ctcaattctt ccttggttct ctcgaagatg aggatgttgg cgtctacggc
     2281 cataccgatg gagaggatga agccggcgat accggccagg gtgagtgtga ccggtacggt
     2341 cttgaagatg gccagcgtag cgatggtgta gagaatcagg gccaggtcgg caatgagacc
     2401 gggcagacca tagatggcca ccatgaagat catgaccgcg gcgataccga cgcaaccggc
     2461 cacgaggctc ttttgaatgg agtcttgacc gagggtggcg cccacggagc gctcttccaa
     2521 aatttc
//
LOCUS       DAOQNZ010000535         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_23951_length_2524_cov_3.795464, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000535 DAOQNZ010000000
VERSION     DAOQNZ010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23951_length_2524_cov_3.795464"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2524)
                     /locus_tag="PKW73_10165"
     CDS             complement(<1..>2524)
                     /locus_tag="PKW73_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNA73694.1"
                     /translation="LNLVAGRRAKANTAYNFAIEIFEQGVSLLNEEFWQNAHQLCFNL
                     IFDLAECNWMCGNFDQAVSLFANLLNHCQTKLEKARIYTMEIELYTGKTELNRAVGRA
                     LEALLLFDIKLSAHPTREEVLKEYEKLWQVLGDRKIEELIALPLMSNEEIKATLDILQ
                     ALYAAALCSDQNLFLLCACHIVTLSLKHGHCDASVMGYGFLGMGLARVFGKYDEAYRF
                     GKLALKLVEDRGLTAYRDRINFIFGDTVNFWVNHLRTNLQYLYSCFESTSRNGDVTYA
                     GYCCNHIVIDLLIWGIPLDEVQAQSEKYLAYCRSVKFDAPAEAIIGMQLFIQNMRGLT
                     DSFTSFDGAIIEDNEPFNETIYESYITQYPQPIVICWYYITKLQARFMSGKYKEAIAA
                     AKKAEPLLWSSLAHIQEPEYWYYYPLALAAQYDQELLKVDDESKCKQLKEAALTIISA
                     HQEQLEQWAQACPDNFRNKHCLVKAELARLEGHLLESERLYEEAIQSARENGFIQNEA
                     LSNELAARFYKGRGFETIANAYLKEAYRCYKQWGAHGKLRQLENQYPVLRQDNPAVAS
                     LDLITVLRAAQAISKEVVLDNLLETLMRTVVEAAGAQSGILFLEQDGELYVRAKAYIK
                     AEEFRSTSQEHRSIAVQIEELALKDGRDLPTSVVNYVRRTHETMVLNDAAREGAGDSL
                     GMLGNDPYFRQRGSRSVLCLPIVKQTKLVGILYLENNLASHLFTADRIDLMQLLSTQI
                     VTALENGLLFAGIQKLNLELEQRVEERTAELAITNSELARAKEAAEAANRAKSSFVAN
                     VSHEIRTPMNAVIGMSDLLSNTALDARQLDMIGTIRESSEILL"
BASE COUNT          559 a          615 c          680 g          670 t
ORIGIN      
        1 ccagcaatat ctcgcttgac tcgcggatgg tgccgatcat atcgagttgc ctggcatcga
       61 gagcggtatt actgagcagg tcgctcatac caattacggc gttcatgggc gtacgaattt
      121 catggctgac atttgctacg aaggaactct tggcgcggtt tgccgcttct gccgcttcct
      181 tggctctggc caattcggaa ttggtaatgg ccagctcagc ggtgcgctct tccactcttt
      241 gttccaactc cagattgagt ttctgaatgc cggcaaagag taagccgttt tccagggctg
      301 tgactatttg cgtggagagc agttgcatca gatcaatacg atctgccgtg aagagatggg
      361 aggcaagatt gttttccagg tagagaattc cgaccagctt tgtttgtttt acgatgggta
      421 gacagagcac tgaacggctg ccgcgctgtc tgaagtaggg atcattgccg agcatgccga
      481 ggctgtcgcc ggccccctct cgggcggcgt cgttaagcac catggtctcg tgtgtgcgtc
      541 ttacatagtt gactaccgaa gtcggcaggt cacgtccgtc cttgagagcc agctcttcaa
      601 tttgcactgc aatggagcga tgttcctggc ttgtgctgcg aaactcctcc gctttgatat
      661 aagctttcgc tcgcacataa agttcgccgt cctgttcgag aaagagaatg ccgctctgcg
      721 cccctgctgc ttctaccacg gtgcgcatca aagtctccag aagattatcg agaactactt
      781 ctttagagat ggcctgagcg gctctcagaa ccgtgattaa atcaagggag gcaacggcgg
      841 gattatcctg tctcagtaca ggatattggt tttccaattg gcgcaacttc ccatgggcac
      901 cccactgttt gtagcagcgg taagcctctt tcaagtaagc gttggcgatg gtttcaaaac
      961 cgcgaccctt gtagaagcgc gccgccagtt cattagacag cgcctcattt tgaatgaagc
     1021 cgttttcccg agctgattga atggcctctt cgtagagcct ctcgctttcc agcaggtgcc
     1081 cttccagccg tgccagttcg gctttgacca ggcaatgctt gttgcggaaa ttatccgggc
     1141 aggcctgagc ccactgttcc aactgctcct ggtgggcgga gatgatagtc agcgccgctt
     1201 ccttcaattg tttgcattta ctttcgtcgt ctaccttgag caattcctga tcgtattggg
     1261 cggccagggc caggggatag tagtaccagt attccggttc ctgtatgtgg gccaggctgg
     1321 accagagcaa gggttctgct ttcttggcgg cggctatggc ttccttatat ttcccggaca
     1381 taaagcgggc ttgcagtttg gtaatgtaat accagcagat gacaatgggc tgcggatatt
     1441 gcgttatgta ggattcgtag atggtctcgt tgaagggctc attgtcttcg attatggcac
     1501 cgtcaaaact tgtgaaagaa tcggtcaggc cgcgcatatt ctgtatgaaa agttgcatgc
     1561 cgataatggc ttcggcggga gcatcaaatt tgacgctgcg gcaataggcc agatactttt
     1621 ccgactgtgc ctgtacttcg tcaaggggaa tgccccagat aagcagatct atgacaatat
     1681 gattgcaaca gtagccggca taggttacat ctccgtttcg agatgtggat tcgaaacagg
     1741 aatagagata ttgtagattg gttctcaaat ggttgaccca gaagttcacc gtgtcgccga
     1801 aaatgaagtt gatgcgatct ctgtaggcgg ttaaaccgcg atcttccacc agtttcaggg
     1861 ctaatttgcc gaagcggtaa gcttcatcat atttgccgaa aaccctggcc agacccatac
     1921 ccaggaagcc gtagcccatg accgaggcgt cgcaatgtcc gtgtttgagg ctgagcgtca
     1981 ctatgtggca ggcgcaaagc aaaaagagat tttggtccga gcatagtgca gctgcataca
     2041 gcgcctgcaa tatgtcgagg gtagctttga tttcttcatt gctcatgagc ggcagagcaa
     2101 ttagttcttc tatttttcta tcaccgagaa cttgccagag tttctcgtac tctttcagca
     2161 cctcctctct ggtggggtgg gccgagagtt tgatgtcgaa aagtaatagg gcttccaggg
     2221 cgcgcccgac agcccggttt agctcagttt tgcccgtata tagctcgatt tccatggtgt
     2281 agatacgagc tttctcaagc ttggtctggc aatgatttaa aagattggcg aagaggctca
     2341 cggcttgatc gaaattaccg cacatccagt tgcactcggc caggtcgaag atcaagttga
     2401 agcagagctg gtgtgcattc tgccagaact cttcgttcag taaagaaacg ccctgctcaa
     2461 aaatttcaat ggcgaagtta taggcggtat tggctttggc tcgacgaccg gcaaccagat
     2521 tgag
//
LOCUS       DAOQNZ010000536         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24004_length_2521_cov_4.277372, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000536 DAOQNZ010000000
VERSION     DAOQNZ010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24004_length_2521_cov_4.277372"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PKW73_10170"
     CDS             <1..1023
                     /locus_tag="PKW73_10170"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNA73695.1"
                     /translation="PETIGEGQWVSASSAEATRYSALGLLFARELHRHVREPIGIIQV
                     SLPKVPIKSWISPTGLDTNGDTRSMAAKNALDEDFYNKLWNEYCQSSDPKKEPATGLT
                     NMASGISNGMLAPLAPYPLKGVLFCQGESDLNFPVQYKTLFPVLLKDLRRLFAQEQLP
                     FYYVQMGAQPSQTADKENMSAAASIRHTQYKARIAPKSYLIVSTDLCHEDMKSGSIYM
                     SVEELAKRVANSTLATLYKVPKPYLYPIVEYVENEGEGIKVVFKNAGRGLMAKGEQPV
                     KGFALAGHDHKFFDADAKIEGNTVFLECKYVKAPRYVRYGWGNNPILTIFSLDGLPAV
                     PYTNDR"
     gene            complement(1154..2131)
                     /locus_tag="PKW73_10175"
     CDS             complement(1154..2131)
                     /locus_tag="PKW73_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNA73696.1"
                     /translation="MSQGILVFVEERGGSLRKASLEALSEAGRRIGAVGGEVTAVLIG
                     SAVKGLAGQLGKYGAAKVIVVENGALEKYSTEAFTQALSEAVKKTSPKYVFAAYTSMA
                     KDFVPRVAARLDAPCVSDVMDFVVDGANLCPVRPMYSGKTFGTYEIKASLSFITLRPN
                     VFALAEGSGDVASEELAVEFAAPKAKVTEVHASEGAKVELSEASIVVSGGRGIKGPEN
                     WPVLQSLCDALGAALGASRAVVDSGWIDHQFQVGQTGKTVSPQLYIACGISGAIQHLA
                     GMSSSKVIVAINKDPEAPIFKHATYGIVGDVLEVVPKLTEAVRKLREHN"
     gene            complement(2156..>2521)
                     /locus_tag="PKW73_10180"
     CDS             complement(2156..>2521)
                     /locus_tag="PKW73_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73697.1"
                     /translation="QTTMVVKFECDGTTFKAEREIEGAHEFVEGTLPAVISAQKGLNE
                     PRYPGIKGVMAARRKEIAVKDAAALGVKGQVGGDKLQVTIKEMALPPERPQGRKIEGD
                     TDSAVSELVNLLRSEAHVL"
BASE COUNT          616 a          738 c          634 g          533 t
ORIGIN      
        1 cctgaaacta tcggagaggg acaatgggtg agcgcaagct ccgccgaggc cacgcgctat
       61 tcggcgttag gtcttctatt tgccagagaa ctgcaccgtc acgtaagaga gcccatcggc
      121 atcattcaag taagcttgcc aaaagtaccg attaaatcat ggatttcccc caccggtctt
      181 gacactaatg gcgataccag aagcatggcc gccaaaaacg ccctcgatga agacttctat
      241 aacaagctct ggaacgagta ctgccagtcc agcgatccca agaaagaacc ggcaaccggt
      301 ctaaccaata tggcatccgg catttctaac ggtatgctgg caccactggc tccttatccc
      361 ttgaagggcg ttctcttctg ccagggtgaa agcgacctga attttcctgt gcagtacaaa
      421 accctcttcc cggttttgct caaagacctg cgccgcctct tcgctcaaga gcagttgcct
      481 ttctactacg ttcaaatggg tgcccagccc tcccagaccg ccgacaaaga aaacatgagc
      541 gcagcagcct ccatcaggca cactcaatac aaagccagaa tcgcacccaa aagctacctg
      601 atcgtctcca ccgacctctg ccatgaagac atgaagagcg gcagtatcta tatgagcgta
      661 gaagaactgg ccaaacgggt agctaattcc accctggcta cactctacaa agtacccaag
      721 ccctacctct accccattgt cgaatacgtt gaaaatgaag gcgaaggaat caaggtcgtc
      781 ttcaagaatg caggccgcgg tctgatggct aaaggcgagc aaccggtaaa aggtttcgcc
      841 ctggccggtc acgatcacaa attcttcgat gccgatgcaa aaatcgaagg caacacggtt
      901 ttcctggaat gcaagtacgt gaaagcgccc cgctatgtgc gctatggctg gggtaataac
      961 cccatcctca ctatcttttc cttagacggc ttgccggccg taccttacac caacgaccgc
     1021 tagcaaaaca cagcttcacg ggccgccctc atccggatcg ccccaggttt atcttcaaag
     1081 gctctctagc ccccagaaaa gaaaggggaa gtaccatggt acttcccctt gaataagcag
     1141 gaccttccgc tagttagttg tgctcgcgca gtttacgcac agcctcggta agcttgggaa
     1201 caacttccag cacatcgccg acgataccgt aggtggcatg cttgaagatg ggcgcttccg
     1261 gatctttgtt gattgctacg atcactttgg atgaggacat gccggccaga tgttgaatgg
     1321 caccggaaat gccgcaggcg atatagagct gaggcgagac ggtcttaccg gtctgcccca
     1381 cctggaactg gtgatcgatc caacccgagt ccacaacggc ccgagaggct cccagagccg
     1441 caccgagggc atcgcagaga gactgcaaga ccggccaatt ttccggtcct ttgatgccgc
     1501 gtccgcctga tacaacgata gaagcctcgg aaagttctac cttagcaccc tcggaggcat
     1561 gcacctcggt caccttggcc ttgggagcgg caaactccac ggccagttct tcagaggcca
     1621 catcgccact accttcagcc agggcaaaga cgttcggacg caaggtaatg aaactcaaag
     1681 aggctttgat ttcataggtc ccgaaggttt tgcccgagta catggggcgc accgggcaga
     1741 ggttagcgcc atccaccaca aaatccatca cgtccgacac acagggagca tcaaggcggg
     1801 ctgccactct gggaacgaaa tctttagcca tggaggtgta agcggcgaag acatacttgg
     1861 gagaagtttt cttcacagct tccgaaagcg cctgggtaaa agcttcggtg ctgtattttt
     1921 ccagagcgcc gttttccact acgattacct tggcagcgcc atatttaccg agctgaccgg
     1981 ccagaccttt gacggcactg cctataagca cggcggttac ttcgccgcca acagcgccga
     2041 tgcgacgacc ggcttcagag agagcttcca gagaggcttt acgcaacgag ccgccccgct
     2101 cttctacaaa taccaaaatt ccttgcgaca tttgttttct ccagataatt gaaacttaga
     2161 ggacgtgcgc ttcgctgcgc agaagattga cgagttcgct cacggccgaa tcagtatcgc
     2221 cttcaatctt gcgaccctgg ggacgctcag ggggcagagc catctctttg atggtcacct
     2281 ggagcttgtc gccgcctacc tgtcctttga caccgagagc agcggcatcc ttgacggcga
     2341 tctctttacg gcgggcagcc ataacgcctt tgatgccggg gtaacggggc tcattcagac
     2401 ctttctgggc gcttatcacg gccggcagag tgccttcaac aaattcatgg gcgccttcga
     2461 tctcgcgctc ggctttgaaa gtggtgccgt cgcattcaaa tttaaccacc atggtggtct
     2521 g
//
LOCUS       DAOQNZ010000537         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24068_length_2518_cov_4.036541, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000537 DAOQNZ010000000
VERSION     DAOQNZ010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24068_length_2518_cov_4.036541"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..951
                     /locus_tag="PKW73_10185"
     CDS             529..951
                     /locus_tag="PKW73_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73698.1"
                     /translation="MPSNSIFAIAAIAVLILIVLVLKKGRSDRAKNQPAKLVEAKKNT
                     LRSELHDLGLGIRQVQGYIDALKGDEKATVEARQLAQAELDKASELKASIEQSMENAT
                     TDAQVDGFALGLEHARLHVRKARSQALFNKQDEDDEDN"
     gene            993..1460
                     /locus_tag="PKW73_10190"
     CDS             993..1460
                     /locus_tag="PKW73_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73699.1"
                     /translation="MKTVLFLLIISGLLILLNMSRPPRPRNKPSSRKPEGKPEQDPAA
                     DAKAIAPAVSEKCNCQHGPVEPEHPLIKVINLTSRIDVIKKEVEALPEDPATLSERRI
                     LKEQVRMATFERDLLLSVLTGHHPNPSAINTDWCFTSAELYLKFARDRLDRIS"
     gene            1475..2233
                     /locus_tag="PKW73_10195"
     CDS             1475..2233
                     /locus_tag="PKW73_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73700.1"
                     /translation="MKTLVRLACLGLIGFLLCMVISLWNISSDPGLSGMFLGLAIPCA
                     TLFFGGMLGASSHIEETEGERAARCLHTARWLNRELLDNIKATDAIVKEAEDLSRKLS
                     ETAYDSAMLTRYQHDEATLNRLRLEFATTVDTTQSGVKEAQYLLDRLAAHDRASRRLL
                     RLSNQAESKIRMANLQSGGKTWMAGPSVAGQYILVGEVEPQRILIVTNLAFKASVPRI
                     EAIKQELAYARTQSQQCLDRLSAYMTSPKQASKP"
BASE COUNT          631 a          780 c          629 g          478 t
ORIGIN      
        1 aggcgcatcc tttcggccga cgctcaggga caggaattgc gtcttctcaa aatgagcagc
       61 ctggaggaga ctgccaatcg cggcgaccgg ctgcgccggg aattgtagag ccgagttgac
      121 aggcgccctt cggcaaaccg acatcctgga gagaaatacc tcgtcatcat tccctgatca
      181 atacgacgcg cagagggatg cgcatagccg cgccaccctg acatccccac aaaaccgcca
      241 gaggagccac accggaagca tatacacggc accaccatga caaccacgca taccgggaac
      301 ctgcttgaaa cccttaccga tacggacttt ctcttgaatc catgaaagcc tgatcaaata
      361 tcgagagctt tttctaactt gagcaaaggg catcaatctt gatagtcaga atctgacttt
      421 tcttccaata ctcacttcga cttaaaacca cccccaatct gtcgagacta tctcccttaa
      481 acaatttcga tgaacgcctc cagccggagc gtttgtcagg agaaaaatat gccttctaac
      541 agcatcttcg ccatcgcggc gattgcagtg ctcatcctca tcgtcctggt tctgaaaaag
      601 ggtcgtagcg accgtgccaa gaaccaaccg gctaagctcg tcgaagccaa gaagaatacg
      661 ctgagaagcg aactccatga cctgggtctg ggtatccgtc aagttcaagg ctacatcgac
      721 gccctcaagg gtgatgagaa agccaccgtg gaagcgcgtc agctagccca ggctgagctc
      781 gacaaagctt ccgagttgaa agcctccatc gagcagtcca tggaaaatgc cactaccgac
      841 gctcaggtgg acggcttcgc cctcggcctc gaacatgctc gcctgcatgt gcgtaaagcc
      901 aggtcccaag cactcttcaa caagcaagac gaggacgacg aagacaactg agtctgtctc
      961 gctcgagctg aaattcacgg aaagaaataa ctatgaaaac cgtactgttt ctgcttatca
     1021 tctcgggtct tctcattctg ctcaatatgt ctcgaccgcc tcgcccgcgc aacaaaccct
     1081 ccagtcgcaa gccggagggc aaacccgagc aggacccggc cgcagatgca aaggcaatcg
     1141 ctccggcggt ctccgagaaa tgcaactgcc aacatggccc tgtggagcca gaacatccgc
     1201 tcatcaaggt catcaacctc acaagtcgga tcgacgtcat caagaaagaa gtcgaagccc
     1261 tgcccgaaga tcccgccact ctctcggagc ggcgaatcct gaaggaacag gtgcgcatgg
     1321 ctactttcga gcgagattta ctcctttcag ttcttaccgg acatcatccc aatccgagcg
     1381 ccatcaatac cgactggtgc ttcacgtccg ccgaactcta cctcaagttt gccagggata
     1441 gactcgaccg gatttcctga gacgcgagaa atacatgaaa accctagtca gattggcctg
     1501 cttggggctt atcggctttc ttctgtgcat ggtcatatcc ctctggaaca taagcagcga
     1561 cccgggcctc tccggcatgt tcctgggctt agcgataccc tgcgccaccc ttttcttcgg
     1621 cggcatgctg ggtgccagct cccatatcga ggaaaccgag ggagagcgtg ccgcacgctg
     1681 cttgcatacg gctcgctggc tcaatcgtga gcttctggac aacatcaagg ccaccgatgc
     1741 catcgtcaag gaggcggagg acctgagccg caaactctcc gaaacggctt acgacagcgc
     1801 catgctgacg cggtaccagc acgatgaggc gactctgaac cggctcaggc tggaattcgc
     1861 cactactgtt gataccacac aaagcggcgt caaagaagcc caatacttgc tcgaccgcct
     1921 ggccgctcat gaccgtgcct ccaggcgctt gctcagactc tccaatcagg ctgagtcaaa
     1981 gattcgcatg gcgaatctgc agtccggggg caaaacctgg atggcaggtc cgtctgtggc
     2041 cgggcagtac atcctggttg gagaagtgga gccccagcgc atcctcattg tcaccaacct
     2101 ggctttcaaa gcttcggtgc cccgcatcga agcgatcaag caggaactcg cctatgccag
     2161 gacacagtct caacagtgcc tggacaggct ctctgcttac atgacgagcc ccaagcaggc
     2221 tagcaagccc tagggtcacg tccaaatcag tccggttgcg cctgtcggag ccaccgggct
     2281 gctttctaag tactccgaca aaacaccgaa gcagtcagga cttacagttc tgactgtttc
     2341 accacaaaca acccaatcaa aatcccgaaa ggatctcatc atgacctcca agaacaagaa
     2401 ggccggcggc tgcggctgcg gcggcaactg cggcaacaag aagtgcaaga agtgcgccga
     2461 tgcggccaag aacgctgcgg ccaacactcc ggctgctcct gccaccaccc cggccacg
//
LOCUS       DAOQNZ010000538         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24094_length_2517_cov_3.732738, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000538 DAOQNZ010000000
VERSION     DAOQNZ010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24094_length_2517_cov_3.732738"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1032)
                     /locus_tag="PKW73_10200"
     CDS             complement(22..1032)
                     /locus_tag="PKW73_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73701.1"
                     /translation="MLDKAASRSLTSHDLNEIYYGSYECSDLLMPCINLDAKFISDNS
                     NLEIYPLHGLLNNSQYQSIVGKLINSPHSYNQALGFIVAASAEDRTYVDKMLAALRKD
                     EDYPLRTYGAAALLYLKHPDPQLLFEYLINHQNIEDLDLWSTFFWQGESALYAMSLKK
                     LASTNPTACTTALALHAPMQDKNAPLPGCEAEFEESLKHLNYPLTDQYKDTRLYIKSK
                     GNALYVNTNWNGLNIYKGHQLLLKDCHDFNDLARQATPVYYIDKTPQEKKARLYASMN
                     VLIHESFFHDRRWQDFDFGPLTNAVLIKNGKAQPIARDSIKTKKWKKTSTSKDHNMNQ
                     VD"
     gene            1362..>2517
                     /locus_tag="PKW73_10205"
     CDS             1362..>2517
                     /locus_tag="PKW73_10205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA73702.1"
                     /translation="MAEKSEITRQLEKQGLGFTVCTSCEEVLAIEGGSLAAAPGLPQG
                     LVPAFAPSPSDLRRCSRDGAPLLRCPPVGEILVGDYQFLSLIGVGGMGGVFKCRHRLL
                     DKTVAVKRLLDESVSERDKLRFKQEAQVLSKLQHKNLVTVYDFGFDGAGLPYLAMEYI
                     EGQTLSQILKLRQLSLEESLLIFEQCLAGLECAHRAGVIHRDIKPGNLILRGAYPDLE
                     LKILDFGIAKAATGAGGREGQSLTSTGEVFGSPLYMSPEQALGRAVDERTDLYSLGCV
                     LFEMLFGAPPFRGANSMETVFKHVNEEPVVPVRTAGGASPGMRLTGLLASMLSKDPAA
                     RPRSAHDCRLSLEAIRSLGPSAEAGTDGRNLKRQVEPEADESGAADSPAVA"
BASE COUNT          504 a          563 c          759 g          691 t
ORIGIN      
        1 gtttgtcagg gcggccgggg gctaatcaac ctgattcatg ttgtggtctt tagaagtgga
       61 agttttcttc cacttctttg tcttaattga gtctctggca atcggctgcg ctttgccgtt
      121 tttaatcaag acggcattgg tgagcggtcc gaagtcgaag tcttgccagc gtctgtcatg
      181 gaagaagctc tcgtggatga ggacattcat ggaagcatag agcctggctt tcttttcctg
      241 cggcgtttta tcaatgtagt aaacaggggt ggcttgcctg gcaagatcgt tgaagtcgtg
      301 gcagtccttc agtaagagct ggtgaccttt gtagatgttt agcccattcc agtttgtatt
      361 tacgtagaga gcatttccct ttgatttgat gtagagcctg gtgtctttgt attgatccgt
      421 gaggggatag ttgaggtgct tgagactttc ctcgaattcc gcctcgcatc ccggcagcgg
      481 cgcgttttta tcctgcatcg gtgcatgaag tgccagggct gttgtgcagg cggtgggatt
      541 tgtcgaagct agttttttca gagacattgc gtataaagca gattcgccct gccagaaaaa
      601 ggtggaccag aggtcgagat cttcgatgtt ttgatggttt atcaggtact cgaacagcag
      661 ttgaggatct ggatgtttca ggtagagcag ggcggctgcc ccgtatgttc gaagcggata
      721 gtcttcgtcc ttacgcaggg ctgcaagcat tttgtctaca taggttctat cttcggccga
      781 ggcggccact atgaaaccca gggcttgatt gtatgaatgg ggcgaattga tgagcttgcc
      841 gacaatagat tggtattgtg aattgttcag caggccatgc aggggataaa tttccaaatt
      901 ggaattatcg gagatgaatt tggcatccaa attgatgcag ggcatgagca ggtcgctgca
      961 ttcataggag ccgtagtaga tctcattgag atcgtgactg gttagcgatc tgcttgccgc
     1021 tttgtccagc aaggttaaga gcttcttttg ctctggactt agattaccgt tgtctggcac
     1081 ggcgctgatg ggagaagcgg tttgacttgc tttctgctgc ggtgccgcta agctcgcgcg
     1141 ggcgttgttt gtgagcagaa gcaggcaagc ggccagagat acttgagttt taagctcgtg
     1201 agcgcggaga gtccggctta acatgcggcg gttcctgtca aaaatgcaaa cgtgcatctt
     1261 tgacatttta cttcctggtt tggtttgccg ctaaccccta tgtttttgtg ttttttagag
     1321 gaattggata tgcttgtgct gctttatgac ggatgtggct tgtggcagag aaaagcgaga
     1381 ttaccaggca gttagagaag cagggcttag gctttactgt ttgcacgtct tgtgaagagg
     1441 tactggctat cgagggcggc agtcttgctg ctgcgcctgg cttaccgcag gggctggtgc
     1501 cggctttcgc tccctctccg tccgatctgc gccgctgcag ccgagacggt gctccgcttt
     1561 tgaggtgtcc accggtaggt gaaatcctgg taggagacta tcagttcttg tcgctcattg
     1621 gtgtgggcgg catgggcggt gttttcaaat gcagacatcg cttgcttgat aaaacagttg
     1681 ccgtcaaacg acttttggac gagtctgttt cagagcgcga caaactgcgt ttcaagcaag
     1741 aggcgcaggt gctgagcaag ctacagcaca agaatcttgt cactgtctat gattttggtt
     1801 ttgatggggc cgggctgccg tatctggcca tggagtacat cgaggggcag actctttctc
     1861 agatacttaa attgcggcaa ctctcgttgg aagagagtct tttgattttt gagcaatgcc
     1921 tggccggtct ggagtgcgct catcgagccg gggttattca ccgggacata aagccaggca
     1981 atttgatcct cagaggcgct tatcctgatc tggagttgaa aattctcgac tttggcatag
     2041 ccaaggcagc cactggtgcg ggaggacgag aagggcagag tctgacttca acaggtgaag
     2101 tctttggcag tcccttgtat atgagcccgg agcaggccct tggccgtgcc gtcgatgagc
     2161 gaaccgacct ctattctctc ggctgtgtgc tcttcgaaat gctgttcggt gccccgcctt
     2221 tccggggtgc aaactccatg gagactgtct tcaagcatgt caatgaggag cctgtggttc
     2281 ctgtccgcac tgccggcggt gcctcaccgg ggatgcgcct gaccggcttg cttgcgtcta
     2341 tgctctcgaa agatccggct gccagaccga ggtctgcaca cgactgccgg ctcagtctgg
     2401 aagcaattcg tagcctgggg ccgtctgcgg aagcaggcac ggatgggcgg aatctaaagc
     2461 gacaggttga gcccgaagcg gatgaaagtg gtgctgccga ctcacctgcc gtggcgg
//
LOCUS       DAOQNZ010000539         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24151_length_2514_cov_3.577064, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000539 DAOQNZ010000000
VERSION     DAOQNZ010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24151_length_2514_cov_3.577064"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1286)
                     /locus_tag="PKW73_10210"
     CDS             complement(60..1286)
                     /locus_tag="PKW73_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNA73703.1"
                     /translation="MPANTELITKESAAFAPRAGRKQAPALRIAFLVWQTALYLTLAV
                     FLLEAFFYFARVGDSEHTRPDRKVGYKLFANKRITQRKEGFGCFKLNSFGMQNDEVPV
                     AKAPGVFRVAVFGDSYVEALHVPRKSNFLSVIEERLRKETGRPVEVLNFGVSNYSVAQ
                     DYLRYQTLGRQFKPDLVVQTFRVEEIGKLLPVNTTNLLFIRPVYFADYDGHIKYDDTC
                     VRNYYLSREGKRMLATDWLREYSRIWGVIGLMRENWASFSETVKPAMSLLKGGAAAPA
                     ATFTLPTDETRKNYANCYWYMMDAQLAAFARDCRADGAQFMLLRTPMIRPGMHELTDN
                     PTETALLDKTARKLNVPLLNLDQDYRSFVGVTTDDGTNFSAGGHFTAPMHRWVGERVA
                     AFLLQEQVLEARTGAK"
     gene            complement(1295..>2514)
                     /locus_tag="PKW73_10215"
     CDS             complement(1295..>2514)
                     /locus_tag="PKW73_10215"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA73704.1"
                     /translation="FKYTDFAIASWNKAVAISAPWLHLNVQTMSFPLQHVLLPLGISF
                     FVFEFIHYIVDVYKGSKTIGNFIHFSLFAAFFPSQIAGPIKRYQDFIKQLETARVFSS
                     RNLELGLCLILQGLFKKVALADNLSPIVAQGFAHSASLGFAESWIASLAFALQIYCDF
                     SGYTDMGRGSALMMGYDLPDNFNYPYIARSLSDFWKRWHISLSSWLRDYLYIPLGGSK
                     CSMPRRYTNLFLTMLLGGLWHGASWHFVVWGAFHGLGLIVCHTYEHLLNVSAGPISSA
                     LKGFHASLPGKAFSMLATFFFVLVAWVFFRAENLSQAFNVLSAMFAPHAVSTWLALDL
                     ERYPIMAAVVAYSVYRLVWDRREEIALKLPLLVKEAMAPLWPARVVIYLAVFVMATGL
                     APSQASPFIYFAF"
BASE COUNT          697 a          661 c          670 g          486 t
ORIGIN      
        1 ttcgcaccca ggacatgatg agcaaacttt ccgacagcaa gaaggcggaa tagcaccagc
       61 tacttggcgc cggtccgtgc ttccaatacc tgttcttgca agagaaaagc cgccactctc
      121 tcgccgaccc agcggtgcat cggtgccgta aaatggccgc ccgccgagaa gttcgtgcca
      181 tcatcggtgg tgacaccgac aaagcttcta tagtcctggt caagattgag caagggcaca
      241 ttcagcttcc tggcagtctt atccaatagt gcagtctctg tgggattgtc ggtcagttcg
      301 tgcataccgg gacggatcat aggggtgcgc agcaacataa actgagcgcc gtcagcccgg
      361 cagtcccgag cgaaagcagc cagttgcgcg tccatcatgt accagtagca gtttgcataa
      421 ttctttctgg tctcatcggt gggcagagtg aaagtagcag ccggagcggc agcgccacct
      481 ttgagcagcg acattgccgg cttcactgtt tcactgaaag aagcccagtt ttctctcatc
      541 aggccaatca caccccagat acgactgtac tcgcgcagcc aatcggtggc aagcatgcgc
      601 ttgccctccc tggacaaata gtaattacgc acacaggtgt cgtcgtactt gatgtgcccg
      661 tcgtaatctg caaaatagac aggacgaatg aagagtaaat tggtggtatt aaccggcaag
      721 agtttgccaa tttcctctac gcgaaaggtc tgaacgacca gatcgggctt aaactgccgt
      781 ccaagcgttt gatagcgcaa gtagtcctgc gccaccgaat aattggaaac accgaaattg
      841 agcacttcca ccggacgccc ggtctccttg cgcaagcgct cctctatcac actgaggaaa
      901 ttggatttcc tcggcacatg cagagcttcc acataggaat cgccaaagac ggcaacgcga
      961 aagacgcccg gtgccttggc aacgggcact tcatcgtttt gcatgccgaa ggaattcagc
     1021 ttaaagcagc cgaagccctc cttgcgttgg gtaatgcgct tattagcgaa gagcttatag
     1081 cccaccttgc gatcaggacg agtatgttca ctgtccccca cccgcgcaaa atagaagaaa
     1141 gcttccagaa gaaagaccgc aagagtaagg tatagggccg tttgccagac aagaaaagcg
     1201 atgcgcaatg ccggagcctg cttccgtcct gccctggggg caaaagccgc tgactctttg
     1261 gtgataagct cggtatttgc cggcataacc tactctaaaa cgcaaagtaa atgaagggag
     1321 aggcctggct gggagccaat cccgtcgcca tcacaaaaac agccaggtaa ataaccactc
     1381 gcgccggcca gagcggtgcc attgcctctt tcaccaggag cggcaacttg agcgcaattt
     1441 cttcgcgacg atcccagacc aggcggtaga cactgtaagc gaccacggca gccatgatcg
     1501 ggtaacgctc caggtcgagc gccaaccagg tagacaccgc atgcggtgca aacatagcag
     1561 agagtacatt aaacgcctgt gagagattct cagcccggaa gaaaacccag gctacaagga
     1621 caaagaaaaa tgtagccagc atactgaaag ccttgcccgg caggctggca tggaagccct
     1681 tcaaagcact ggagatggga ccggctgaga cattgagcaa atgctcgtag gtgtgacaga
     1741 caatcagacc caagccgtga aaggcacccc agaccacgaa atgccaggaa gcgccgtgcc
     1801 agagcccgcc caggagcatg gtcaggaaca gattggtata gcggcgaggc atactacatt
     1861 tgctaccgcc cagaggtatg tagagatagt cgcgcaacca ggacgaaagc gagatatgcc
     1921 agcgcttcca gaaatcagag agacttctgg cgatgtaggg ataattaaag ttgtccggca
     1981 ggtcataacc catcatcaac gcggacccgc gccccatgtc tgtgtagccg gagaagtcgc
     2041 aatagatttg cagagcaaag gcaagagagg caatccagct ttcagcaaaa ccaaggctgg
     2101 cggaatgagc aaaaccctga gccactatcg gggagaggtt atccgccagg gccactttct
     2161 tgaacaagcc ctgcaaaatg agacatagtc ccagttccag gttacgactg gaaaagactc
     2221 ttgccgtttc caattgcttg atgaaatctt gatagcgctt aatcggaccg gcgatttgcg
     2281 aaggaaagaa agcagcaaag agagagaagt gaatgaagtt gccgatggtc ttggagcctt
     2341 tgtacacatc cacaatatag tgaatgaatt cgaagacgaa gaaggaaata cccagaggca
     2401 atagcacgtg ttgcagcggg aagctcatgg tctgaacgtt taagtgcaac cagggagctg
     2461 aaatagcaac ggccttattc caactggcta tggcaaaatc ggtatatttg aagt
//
LOCUS       DAOQNZ010000540         2508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24269_length_2508_cov_2.541786, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000540 DAOQNZ010000000
VERSION     DAOQNZ010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24269_length_2508_cov_2.541786"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKW73_10220"
     CDS             <1..458
                     /locus_tag="PKW73_10220"
                     /inference="COORDINATES: protein motif:HMM:NF025731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HNA73705.1"
                     /translation="LLNIVVNAAEILFFSAAFPMFIFVALPSLLGFKGYSLRMFLCII
                     FSATIGLVSPTLVEAVVSVLRTNSSPLGLVMLILTIVFIPLLSFGCIGYLAFLPSLIA
                     RSRGLKRRRVIFLVNLLTFFIPLSWLALMIWCYFEAKKEREQVDSQQLN"
     gene            500..1534
                     /locus_tag="PKW73_10225"
     CDS             500..1534
                     /locus_tag="PKW73_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73706.1"
                     /translation="MIITENFPLPLQELAARSGGAPAIDGNSILCLADWPASFWLKLD
                     VSTRIVKGTLEGKLHLPARSNGRLFIFEPGFPGRGSADLEKSHVKHLTEAGYTVFAPR
                     HNGMLLTGKHSDFYISCPERQAKAGQEGQVVLGDGPYTELDKWLLEPLIAIEALSACF
                     EETVLLGHSFGGLALMYSAVKYFAESPGHNIKRLISMAGATGRIRSDSDRIVEMWTEY
                     IDTEYIRERVSIGKAEDNLRHLRHAYGLIHEKIKVLPDQVELLCLHPWGDSHDSIDEL
                     IGVNEPLELILSLGRGTLVVDKTQKAEPSLEQLAHDMCGLSSAALLKLLDTTWKSDKQ
                     ILQLDNSGIH"
     gene            complement(1539..>2508)
                     /locus_tag="PKW73_10230"
     CDS             complement(1539..>2508)
                     /locus_tag="PKW73_10230"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HNA73707.1"
                     /translation="LYRHDSALGDLIADAFLDAGQAQGVQVAMENRGGIRSFIDRGPI
                     TLEKIEEILPFDNHLACATVTGKSLLRNLEHSVAGSLGGRFLDVSGIKFAYDIEKAPG
                     SRIVFALVKDKEGNWKRVEDAATYRIGMTDYSFNGGEGYDFSSAADVTYSQEKLNEPL
                     RRFFLKQKSVRPQAPNRICATVGALTGFLTSKTIVLGGGLSFISKIPNQRLSLYRSNE
                     LGVTTIRPGIVVPLAAPQVVLENIEKEDFPVRVRSYLNRERPARERQAAGSEYYVVVL
                     SGSNEKNPESRSRRAYSSIPISTYDLERLTGINLHDTKTGRSLERH"
BASE COUNT          529 a          617 c          685 g          677 t
ORIGIN      
        1 ccttgctgaa catagtggtg aatgctgcag agatcctctt cttctctgcc gcatttccaa
       61 tgtttatctt tgtggctttg ccctcgctcc tgggctttaa gggttactca ttgaggatgt
      121 ttttgtgcat cattttttcc gccacaattg gactagtcag tccaacgctg gtggaggcgg
      181 tcgtgagcgt gctacgcacc aatagttcgc cgcttgggct tgtgatgctc atcttgacga
      241 ttgttttcat tcctcttctc agctttggtt gtattggcta ccttgccttc cttcccagtc
      301 tgattgctag atccaggggc ttgaagcggc ggcgggtaat ttttctggta aatttactga
      361 ccttcttcat tcctctttca tggcttgctc tcatgatttg gtgctatttt gaggcgaaga
      421 aagaaagaga gcaggtagat tcgcagcagt tgaattgaat acggcccagg tgtcagcact
      481 tattgagaga aggtagcttg tgattattac tgagaatttt ccattgccgc tgcaggaact
      541 ggccgccagg tccggtggcg ccccggcaat tgatggcaat agcattctat gcctggctga
      601 ctggcccgct tccttctggc tgaaactaga tgtctcgact cgtattgtca aaggaacgct
      661 ggaagggaaa ctccatcttc ccgccaggtc caacggacgt ctctttatct ttgagccggg
      721 ctttccgggt cgaggctccg ccgatcttga gaagtcccat gtaaaacatc tgactgaagc
      781 cggctatacg gtatttgcgc ccaggcataa cggcatgttg ctcaccggca agcattccga
      841 cttctatata agttgtcccg agcggcaagc aaaggcaggg caggaaggac aggttgttct
      901 cggtgacgga ccctacacgg agttggataa gtggcttctg gagccgctca ttgctattga
      961 ggccctgagc gcctgttttg aagagactgt gcttctgggg cattcgtttg gcggtctggc
     1021 tttgatgtat tcggccgtaa aatattttgc tgaaagtcca gggcataaca tcaagcgtct
     1081 catatccatg gccggggcca cagggcgcat ccgctccgac agcgatcgca tcgttgagat
     1141 gtggaccgaa tacattgata ccgagtacat ccgcgaacgt gtgtcaatcg gtaaggcgga
     1201 agacaatttg cggcatctgc gccatgccta cggtctaatt cacgagaaaa taaaagttct
     1261 gccggatcaa gtcgagcttc tctgcctgca tccctggggc gatagtcatg acagtattga
     1321 tgagttgatt ggtgtaaacg agcccctgga gctaatcctc agtttggggc gcggtactct
     1381 ggtggtggat aagacccaga aagccgagcc gtctctggag cagttggcac acgatatgtg
     1441 tggtctcagt tctgctgcct tgctaaagtt gcttgatacg acctggaaga gcgataagca
     1501 aattcttcaa cttgataata gcggcatcca ctaggctctc agtgacgttc aagagaccgg
     1561 cctgtttttg tatcgtgtag attgataccg gtgaggcgct caagatcata ggtgctgatg
     1621 ggaatggaag aataggctcg ccgacttctg ctttcggggt tcttttcgtt ggaaccgctc
     1681 aggaccacta catagtattc gctcccggcg gcttgccgct ctcgtgccgg tctttcgcgg
     1741 ttaaggtagc ttcttactct aaccggaaag tcttctttct ctatattttc cagcaccact
     1801 tgcggtgcgg cgaggggcac tacaataccg ggacggatgg ttgttacgcc cagctcattt
     1861 gacctgtaca aagagaggcg ctggtttgga atcttgctga tgaaggaaag cccgccgcca
     1921 agtacgattg tttttgaggt aagaaagcct gtcagggccc cgaccgtggc gcagatgcga
     1981 ttgggagctt gagggcgaac gctcttctgc ttgaggaaga agcggcgcaa aggttcgttc
     2041 agcttttcct ggctgtaggt tacatctgcg gcggaggaaa agtcgtagcc ctctccgccg
     2101 ttgaaactat aatcggtcat gccgattcta taggtggcgg cgtcttccac ccttttccag
     2161 ttgccttctt tgtctttgac gagagcaaaa actatgcggc taccgggagc tttttctatg
     2221 tcgtaggcga atttgatacc actgacatcg aggaatcgtc cgcccaggct gccggccacc
     2281 gaatgttcca ggttccgtaa aaggctctta cccgtaaccg tagcgcaggc caggtgattg
     2341 tcgaagggaa gaatttcttc gattttttcg agggtgattg gcccacgatc tatgaaagat
     2401 ctgatgccgc ctcgattttc cattgccacc tgtacaccct gagcctgacc ggcatcaagg
     2461 aaggcgtcgg caatcaaatc tcccagcgcc gaatcatggc ggtacagg
//
LOCUS       DAOQNZ010000541         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24402_length_2500_cov_4.629039, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000541 DAOQNZ010000000
VERSION     DAOQNZ010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24402_length_2500_cov_4.629039"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1042
                     /locus_tag="PKW73_10235"
     CDS             101..1042
                     /locus_tag="PKW73_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73708.1"
                     /translation="MKYFPKLYSLSRLWAILADVAAVGDVAAVADVAAVRVIAAVTTL
                     AAVIAVEAVAAVAVVAAVTVALPTGNAPIFTSAALAQESVHRGLKVTQQTEASGIYEI
                     WLSPDAIRVSSATAGFVLASRAPKWQVQVWRGDRKERSEVGLAEFLKGDLLLTNHISY
                     AADMRKVLSQKRMTRGTDKLIVYRFPPIEAVGGSGLFTYDSNDDAGNRHGTKLTPELM
                     SLDLGYARPVSQIIAKLHNLIDLPGVPVNAYYYLPGGKSENILDCRILDRRFTFKDSL
                     LTAPASYKKVALSKNLFLTKGQVDMLQDMYGTKVELK"
     gene            complement(1147..2367)
                     /locus_tag="PKW73_10240"
     CDS             complement(1147..2367)
                     /locus_tag="PKW73_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73709.1"
                     /translation="MVSHSNFEISHAPAEASADASSAFHSDYYASTIGARSGQSDAVK
                     FSSAIPAEAPPPAGLSAAAWSQMSQWVGENKQVNISTPGDGQVPDYIIGANGDMVANP
                     AKTTPNPDGSITVQMDGSKGDAASLQAAKELKIAVIQEKIFYWQKAHPNETNIPGYLE
                     GMLAAAQSAPVTPAGGDSQRPQPVQQARPEVQPQPQPMQTAAPSRNYGLGGGEKVGNS
                     SIGTAPTSGYRSGSFEPNGPIDRTAIPRPVEGDLNIKGPPSANADQIQTFLEKMGSPA
                     AKEAGFSQALYTACTERGIDPAVAVGFFLQESTCGRYGRAHDNHSLGNIKGRAPESGG
                     TDGTFRRYDTWAEGARDWARLIDESYVQKRGLSTLSQVIGVYAPSSDGNNENRYVATV
                     KGVVENFKQQNATA"
BASE COUNT          450 a          672 c          748 g          630 t
ORIGIN      
        1 cataagatgc tttttgctcc tttgcactag tggcgttttc cagagccccc agtgcgtata
       61 attgtatttc ttagggcttt tgcggcttga ggaacttgat ttgaagtact ttccgaagct
      121 ttactcactg tcgcgattgt gggccatact cgccgacgtt gctgctgttg gcgacgttgc
      181 tgccgttgcc gacgttgctg cagttcgcgt aattgccgcc gttaccaccc ttgccgccgt
      241 catcgccgtt gaggcagttg ccgctgttgc tgtcgttgcc gctgtgaccg tcgcgttgcc
      301 caccggcaat gccccgattt ttacttcggc ggccctggcg caggagtctg tgcaccgtgg
      361 tctgaaagtg acccagcaga ccgaagccag cgggatctat gaaatttggc tttcgcccga
      421 tgccatcagg gtatcctcgg caactgccgg ttttgttctg gcctccagag cgcctaagtg
      481 gcaggtgcag gtctggcgcg gcgatcgcaa ggaacgttcg gaagtcggtt tggcggagtt
      541 cctcaagggt gacttgttgc ttaccaatca catttcctat gccgcagata tgcgtaaggt
      601 tctctcccag aagcgcatga ccaggggcac tgacaagctc attgtctatc gcttcccccc
      661 gatcgaggca gtgggtggct ccggtctgtt tacctacgat agtaacgacg atgccggtaa
      721 caggcacggc accaagttga cgcccgaact tatgtcctta gaccttggtt atgccagacc
      781 ggtcagtcag attattgcca aactgcacaa tcttatcgac ctgcccggcg taccggtaaa
      841 tgcctattac tatcttcccg gcggcaaatc cgaaaacatt ctcgattgcc gcattctcga
      901 tcgccgcttc accttcaaag attccctgtt aaccgctccg gcttcataca aaaaggtggc
      961 attgagcaag aacctatttc tgaccaaagg tcaggtggat atgctgcaag acatgtacgg
     1021 taccaaagta gagctcaagt agccggcggt ggcttcaggg catcaggcag tggcttcaag
     1081 gcatcaggcg gtggcttcag ggcatcaggc agtggcttca aggcatcagg cggtggcttc
     1141 aaagcgtcag gcggtggcgt tctgctgttt gaagttttct acgacgcctt taaccgtggc
     1201 cacgtagcgg ttttcattgt taccgtcact ggacggcgcg tacaccccga tcacctggga
     1261 aagggtgctc aaaccgcgct tctgcacata ggattcgtca atcagacggg cccagtcccg
     1321 ggccccttcc gcccaggtgt catagcgcct gaacgtgccg tcggtgccgc ctgactccgg
     1381 tgctctaccc ttgatattgc caaggctgtg attgtcgtgg gcgcgaccgt agcggccgca
     1441 agtagactct tgcaggaaga agcccacagc tacagccggg tcgataccgc gctcagtgca
     1501 ggctgtgtag agtgcctgtg agaagccggc ttccttggcc gccggtgaac ccatcttttc
     1561 caggaaggtt tgaatctggt cggcgtttgc cgaaggcggt cctttgatgt tgaggtcgcc
     1621 ctccaccggt ctgggaatgg cagttctgtc gatgggtccg tttggctcaa aactgccgga
     1681 gcgatagccg gaagtcggtg ccgtgccgat actgctgttt cctactttct ctccgccgcc
     1741 cagaccatag ttgcgggacg gtgccgccgt ctgcatcggt tgtggttgtg gttgtacttc
     1801 cggtctggcc tgctgtaccg gttgcgggcg ctggctgtcg ccgccggcgg gagtgactgg
     1861 cgcactctgg gctgctgcca gcataccttc caggtagcct ggaatattgg tttcgttggg
     1921 atgggccttt tgccagtaga agattttctc ttgtatgact gcaatcttca gttctttggc
     1981 ggcctgcaga gaagcggcgt cgcctttgct gccgtccatc tgtacggtga tggagccgtc
     2041 cgggttgggt gtggttttgg ctggattagc taccatgtcg ccattggcgc cgatgatgta
     2101 gtcaggtacc tgaccgtcac caggggttga gatgttcacc tgtttgtttt cgcctaccca
     2161 ctggctcatc tgtgaccagg cggcagcact gagtcctgcc ggcggcggcg cttcggctgg
     2221 aatggcggaa gagaacttaa cggcgtcgga ctggccggag cgggcaccga ttgttgaggc
     2281 atagtagtcg ctgtgaaaag cgctagaagc gtctgcggaa gcttcggccg gtgcatgtga
     2341 aatttcaaaa tttgaatgtg aaaccaactt ctggggcctc ggaaagaaac gggggtaagg
     2401 gggtttggcc gttggatgag gcgaatatat accgcagggg tggttgtgtc tatggtgttt
     2461 ctcccccctt ctgtatgagc gcagtcgacc tgggtatatt
//
LOCUS       DAOQNZ010000542         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24428_length_2499_cov_3.578969, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000542 DAOQNZ010000000
VERSION     DAOQNZ010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24428_length_2499_cov_3.578969"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..1887
                     /gene="murC"
                     /locus_tag="PKW73_10245"
     CDS             391..1887
                     /gene="murC"
                     /locus_tag="PKW73_10245"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNA73710.1"
                     /translation="MELNVNVKTAFLEGISTTAKPDQARRSMNLKDLKGSVHFAGIGG
                     IGMSALARILLNRGYNVSGSDKQESPITDELKALGARICIGHQKENMEGAGALIISTA
                     IVDGNPELAAAREKNLPIYHRSDMLNYLAREDKLISVTGTHGKTTTTALVGQVLLECG
                     LDPSIVVGGIFHHIGANSRTGKGGYFVAESDESDGTHVKSHSFASVITNIEPDHLENY
                     PGGMKEILASMETFVTNTEAFCLVCTDDGGVKQLLSGLSEDNLKKIVSYGKYKEGVAP
                     GELPNYSFKSLPGFDMLVFKGGKELGKVSLRVPGDHNRYNATAAIALAMELDKSFSDA
                     ARAISEFRGVDRRFQLIGQVGDLLVVDDYAHHPTEVAALLKAAQEFKKVERSGKGRVV
                     CIFQPHQPGRLRDLWQEFTTAFDQADQAFISDIYIARGGAIEGISSEAFVKSVKNSHT
                     HYLPGLVNDLAKNLKPFLQEGDLVLTVGAGDITKVGPELVELLQKEGL"
     gene            1884..>2499
                     /locus_tag="PKW73_10250"
     CDS             1884..>2499
                     /locus_tag="PKW73_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNA73711.1"
                     /translation="MTNLPHTFSSNLGAALSSDNGTAKMEAIDNAPLARYTTLRIGGN
                     ADRLYQPESVEELATLIKKLSSEGAPWYVIGGGSNLLISSAGVRGNVVRTTGLTGIKI
                     LAPGLLYAEAGARLPHLAKHAAQQGLAGLEFAVGIPGTVGGAVIMNAGAHGSSISAIL
                     ETAQILDTQTGIISEQSNQDLAFQYRRCALDPARHVVLSAIFRLP"
BASE COUNT          635 a          708 c          639 g          517 t
ORIGIN      
        1 ggaagagaat ttggatgaag gcaaagctac cgaagctgct gatgccatca aaaaagtctt
       61 ctctgtcatt gacagagcgg tgaccaaagg cgttctgcac aaaaacactg cagtcagacg
      121 caaagccaga ttgtatgcgc gccttgttaa agccggcgcc aagaagtaag aagcaagcaa
      181 aatcttatga aagtgacaga cggagagcta ttccgtctgt ttctttttgt ctggatacag
      241 gctatagttt agctgttttg tccttattcc ctacaaaagc cggtccctat gccgtttgta
      301 gcgatattag atataagccc tatatgtaag ttagaaccgt cggtaaaggc agtggcagca
      361 cggaatcagt cctgaaggag aagctaagtc ttggaactca atgtaaacgt caaaacagct
      421 ttcctcgaag gcatcagcac cacggctaaa ccagaccagg ctcggagatc tatgaatttg
      481 aaagacctca agggaagcgt gcacttcgcc ggcataggcg gtatcggcat gtcggccctg
      541 gctagaattc ttctcaacag gggctacaac gtctccggtt ccgataaaca agaaagcccc
      601 atcaccgatg aattgaaggc tctgggagca aggatttgca tcggtcacca gaaagaaaac
      661 atggaaggcg ccggcgccct cattatttcc acggctatag tagacggcaa tcccgagctt
      721 gccgcagccc gagagaagaa cctacccatt tatcaccgct cagatatgct caactatctg
      781 gcgcgcgaag acaaactcat ttccgtcacc ggcacccacg gcaaaaccac taccacagcc
      841 cttgtgggcc aggtcttact ggaatgcggg ctcgatcctt ccatcgtcgt aggcggtatc
      901 tttcaccata tcggcgccaa ctcccgcacc ggcaagggcg gttacttcgt agctgagtct
      961 gatgaatcgg acggcaccca tgttaagtct cactctttcg cttccgtgat caccaatatc
     1021 gaaccggacc acctggaaaa ctatccgggc ggcatgaaag aaatacttgc ttccatggaa
     1081 acctttgtta ccaatactga agccttctgc ctggtctgca ccgatgatgg tggcgtcaag
     1141 caattacttt cgggtctttc cgaagacaac ttgaagaaga ttgtcagcta cggcaagtac
     1201 aaagaaggcg tcgcgccggg cgaactgcct aactacagct tcaagagcct gcctggcttc
     1261 gacatgctcg ttttcaaagg cggcaaagaa ctgggcaaag tcagcctgag agtaccgggc
     1321 gaccataacc gctacaatgc caccgccgcc atagctctgg ccatggaact agacaagagc
     1381 tttagcgacg ccgccagagc gatttcagaa ttcagaggcg tcgacaggcg ttttcaactt
     1441 atcggtcagg taggcgatct actggtggtg gacgattacg ctcaccatcc cacggaagtg
     1501 gccgccctcc tgaaagccgc tcaggaattc aagaaagtag agcgctccgg caaaggtcgc
     1561 gtcgtctgca tcttccaacc ccaccaaccg ggccgcctga gagatctctg gcaagagttc
     1621 accactgcct ttgatcaagc agaccaggcc tttatctcgg acatctacat cgcccgcggc
     1681 ggcgccatcg agggcatcag cagcgaagct ttcgtcaaat ctgtaaaaaa cagccacacc
     1741 cactacctgc ccggtctggt gaatgacctt gctaaaaacc tcaaaccttt cctgcaggaa
     1801 ggcgatctcg tgctgacggt gggcgccggt gatataacga aagtgggtcc cgaactggta
     1861 gaactcctgc aaaaggaggg actatgacca atcttccgca taccttcagt agtaatctag
     1921 gggcagcctt gagttctgat aacggcacgg caaagatgga agcaatagat aatgcgccgc
     1981 tggcgcgcta taccaccctg cgcatcggtg gtaacgccga ccggctctac cagcctgaat
     2041 cggtggagga actggctact ctcatcaaga aactgagcag tgaaggtgcg ccctggtatg
     2101 tgatcggtgg cggctccaac ctgctaatct catctgcggg tgtgcggggc aatgtggtaa
     2161 gaaccaccgg gctgaccggc atcaaaattc ttgcccccgg actgctctac gccgaagccg
     2221 gagcacgcct gccgcacctg gccaagcatg cagcccagca aggtcttgcc ggtctggaat
     2281 ttgccgtggg catacccggc actgtgggcg gtgcggtaat aatgaatgcc ggcgcgcacg
     2341 gcagttccat ttcagccatc cttgaaacgg cccagatcct cgacacccaa accggcatca
     2401 tatccgagca gagcaatcaa gatttagcct tccagtaccg ccgctgcgcc cttgatccgg
     2461 cccggcacgt agtactctcc gccatcttcc gcttacccc
//
LOCUS       DAOQNZ010000543         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24444_length_2498_cov_3.744576, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000543 DAOQNZ010000000
VERSION     DAOQNZ010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24444_length_2498_cov_3.744576"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKW73_10255"
     CDS             complement(<1..549)
                     /locus_tag="PKW73_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73712.1"
                     /translation="MTIETKSKSQRNWTEQFDSAQKHKQNRKYKKALQALEEALALAR
                     KHSLTEETFLSLKALADVQRLSGHLKQAGRAEEEALAMSSQLFAARAAAGGETQPTMA
                     ESKESPEESRMNSNDLKSDRQAKPGKKSDKDKKHDKAEKVKKGEKTDKSKKETKSVKD
                     ETGKKSDKKAKSTKEETRQNESE"
     gene            complement(637..2031)
                     /locus_tag="PKW73_10260"
     CDS             complement(637..2031)
                     /locus_tag="PKW73_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73713.1"
                     /translation="MPLKTITLTLIVSALSAAFFIKPAIAQGSRLGPEAEQHWRQGQR
                     LLATMQPRLALGEFDQALKLAPGNIAILAARGACLMRLKDFEGAYRDFHAALLKSPGQ
                     PDLLRLRGTANMCLCEADRAIDDLSQAIAIKPTAQAIFNRATCYIQTARYEKAIEDYT
                     SLIKRRANLYPSLFQRGRVYYLLDEYDKAIVDFSAVIKADPNSALKCYICRGRAFAAK
                     GRTDLALADLKKAVLLDDDYAESFYAPAVESLIESDARLEERPPANEQKSSTGKLKSL
                     SSKQSPSNQEAGDKYLAEALALKEKKQWARALAACDLALKANARSTTAHVLKGKILME
                     SGNYKAALACFNQAWTMDNLNLDAVRGRAEANSALGLPQRSLADLGKLVLASPDDPEL
                     RFRQAEIFDKLKRPKEALAGYLKCLELDHRKELLKDKLGKDKDAYFRIAQATQALSAE
                     QRRRALARIEILKQ"
     gene            2214..>2498
                     /locus_tag="PKW73_10265"
     CDS             2214..>2498
                     /locus_tag="PKW73_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73714.1"
                     /translation="MTELAKPCDFCNRLGAFVLSALLSSSLPALSQGSVGSGTGASSP
                     TLSVQTGAPADSAAAQAAAAAAKQEAFIVSELQGLHNLNPDPLQTCEAAQP"
BASE COUNT          426 a          665 c          660 g          747 t
ORIGIN      
        1 ctcgctctcg ttctgcctgg tttcttcttt agtggacttg gcttttttat ccgacttctt
       61 accggtctcg tcctttactg attttgtttc tttcttgctt ttgtcggtct tttcgccttt
      121 tttgactttt tcggctttgt cgtgtttttt gtctttgtcc gacttcttgc ctggctttgc
      181 ctgccggtcc gatttcaagt cattagaatt catccggctt tcctcgggcg attctttgct
      241 ctcagccatt gtgggttgcg tttcgccacc tgctgcagct ctggccgcga agagttgact
      301 gctcatggcc agggcttcct cttccgctct gccggcttgt ttcagatgac cactcagcct
      361 ctgcacatca gcaagggcct tcagggaaag aaaagtttct tctgttaaac tgtgttttcg
      421 agcgagcgcc aatgcttctt ccagagcttg cagagccttt ttgtactttc tattttgttt
      481 atgcttctga gctgagtcga attgctcagt ccagtttctc tgagatttgc tcttggtttc
      541 tatggtcatt ggcttcgact tctctacgac attgagggca gagggaattt tactctctta
      601 gaagagattt gtcgtttcgc taaattcaga ttggcatcac tgtttaagga tttcaattcg
      661 cgccagggcc ctgcgtcttt gctccgctga aagggcctgt gtggcctggg cgatgcggaa
      721 ataggcatct ttatctttgc ccaatttgtc tttaagtaac tctttccggt ggtccaattc
      781 aaggcatttc aggtagcctg ccagggcctc ctttgggcgt ttgagtttgt caaaaatctc
      841 tgcctgcctg aatctaagct ccggatcatc ggggctggcc aggaccagtt tgcccagatc
      901 tgccagggaa cgctgcggca gaccaagggc tgaattggct tccgccctgc ctctcactgc
      961 gtctaggttg agattgtcca ttgtccaggc ctgattgaag caagcgagag cggctttgta
     1021 attgccgctc tccatcaata ttttgccctt cagcacatgg gcggtggtgc ttcttgcatt
     1081 ggccttgagc gctaaatcac aagccgccag ggcccgcgcc cattgtttct tctctttcag
     1141 ggcgagagct tctgccagat acttgtcgcc ggcttcttga ttactggggc tttgctttga
     1201 actgagcgat tttagtttgc cggtgctgct tttttgttcg ttggcaggag gtctctcttc
     1261 caatctggca tcgctttcga tcagtgattc cactgccggt gcatagaaac tttccgcgta
     1321 gtcatcatcg agcagcactg cttttttcaa atcggcaagg gccagatccg ttcttccttt
     1381 tgctgcaaaa gcgcgccctc tgcagatata gcatttgagg gcagagtttg gatcggcttt
     1441 gatgacggcc gagaagtcga ctatggcttt gtcgtattcg tcaagcaggt aatagacccg
     1501 gcctctctga aagagcgagg ggtatagatt ggcccgccgc ttgatgagac tcgtgtagtc
     1561 ttctatggct ttttcatacc gggcggtctg tatatagcag gtggcacggt tgaaaatggc
     1621 ttgggctgtg ggcttaattg caattgcctg gctgaggtcg tcaatggctc tgtcggcctc
     1681 gcagaggcac atgttggccg tgccccgcag tctgagcaga tccggctgac cggggctttt
     1741 cagaagggct gcatggaagt ctctgtaggc cccctcgaaa tccttcagac gcatcaggca
     1801 ggcgcctctt gctgccagaa tggcaatatt gcccggtgcc agtttgagag cctggtcgaa
     1861 ctcccccagg gccaggcggg gttgcatggt ggcaagcagc ctttgaccct ggcgccagtg
     1921 ctgttcggct tccgggccga gcctggaacc ctgagcaatg gccggcttta tgaaaaatgc
     1981 cgcactgaga gcgctaacga ttaaggtcag ggtgattgtc ttaagaggca actgtggcaa
     2041 atttccccct gggggagccg tccgtccatt gcgccccgtg acatcccggg gcgagcttaa
     2101 ctagctctga tattagcact ttactattgc tgatccctcc aggtctggtg ttattctgag
     2161 ctctggcagc aatttaagcg ctgcttccac ttcatagttg agagatccaa gttgtgactg
     2221 aattagctaa accctgtgac ttttgcaacc gtttgggcgc ctttgttttg agcgctttgc
     2281 tctcatcgag cctgccggca ctttctcaag gctctgtcgg ctccggcacc ggtgcttcca
     2341 gtcctactct ctccgtacag actggcgctc cggcagattc cgccgcggcc caggctgccg
     2401 ccgctgctgc caagcaggag gctttcattg tcagtgagtt gcaggggctt cacaatctca
     2461 atcccgatcc cctccagacc tgtgaggctg ctcagccg
//
LOCUS       DAOQNZ010000544         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24448_length_2498_cov_3.342202, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000544 DAOQNZ010000000
VERSION     DAOQNZ010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24448_length_2498_cov_3.342202"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1419
                     /gene="aroC"
                     /locus_tag="PKW73_10270"
     CDS             160..1419
                     /gene="aroC"
                     /locus_tag="PKW73_10270"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNA73715.1"
                     /translation="MLRLLTAGESHGPKLTAMIDGMPAGLSIDTAFIEAELAARQGGY
                     GRSGRQKIEKDKIDITGGVRHGLTTGAPVCFEIQNRDHGNWTTVMNSAPIDLSALDEA
                     GKKQIQDKRIERFRPGHADLAGTIKYRLKEVRDVLERSSARETAARVGAGSLAQLILR
                     ALGVKLTAHVVAIGPVSMNSLSREEQERIEAMDLGELLLASRDSSVYCPHEGLSEQME
                     ATILQALKDGDSLGGVVRVMVDGLPPGLGSSFQWDRKLDGQLAQSLMSIQSAKAVEIG
                     TGVENAALPGSLVHDAMHRDESGKSPCYYVGRKTNRCGGLEGGMTNGERLILHVYFKP
                     IPTMKKGLPSLSFPAFTDSFAHYERSDVCAIPAASVVAGAMVSLTLANAFLDKFAADT
                     MVDLKESVETYRQYVRNLTKGKVDNAP"
     gene            1409..>2498
                     /gene="aroB"
                     /locus_tag="PKW73_10275"
     CDS             1409..>2498
                     /gene="aroB"
                     /locus_tag="PKW73_10275"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNA73716.1"
                     /translation="MRHKARHRGPVITMNWNTTLDVKTRVAVGAGARQKLSSILAQVG
                     AGRRVLIVCQPTTAVHWLRDILDTLPTEDFQVTTLEVPDGEQCKTTEWLLRIWEHLEG
                     RGFDRQDTVVALGGGAVLDLAGFACSTYMRGLNLVLIPTSLLAQVDAAIGGKNAINLP
                     TGKNLAGTFFFPKAVLADQEVLGTLPGRQLTAGLAEIIKYALIEETVAKGTDYEKGPK
                     PLLELVEEMVKDPVEYDDPTLSGIITNCIKMKLYVVHRDPHETGIRRSLNLGHTLGHA
                     LEAQSEFELNHGEAVAIGLYHATKYSVGQKKLEKDSLERVKEVLMRAELPYELPKGLS
                     KDRLVQQMNSDKKRSGEKIRLVIPQTRLG"
BASE COUNT          631 a          709 c          694 g          464 t
ORIGIN      
        1 cccggcatct gccataattc ccctgaacta caagcaggca taagttataa ggctaacata
       61 tgcttgccgg atccaagaat caggatctat gacggtaaaa cgggaaacac aacaggaaac
      121 acaacaaact agatccgctc aaggcgggga ggcacaaatt tgctcaggct tttaactgcc
      181 ggagaatcac acggaccgaa actgacggcc atgattgacg gaatgccggc cgggctcagc
      241 atcgataccg cctttataga agcggaactg gccgccaggc agggcggcta cggccgcagc
      301 ggcagacaga aaatcgaaaa ggacaaaatt gacattactg gcggtgtcag acacggtctc
      361 accaccggcg ctccggtctg ctttgagatt caaaacagag accacggcaa ctggacaacc
      421 gtcatgaata gcgctcccat tgacctgagc gctctcgatg aagccggcaa gaaacagatt
      481 caggacaaac gcattgagcg cttccgccca ggtcatgccg acctcgccgg cacaatcaag
      541 taccggctca aggaagtgcg ggacgtactg gagcgctcga gcgccagaga gaccgccgcc
      601 cgggtcgggg ccggcagcct ggcccagctc atcttgcgcg cactgggtgt aaaactgaca
      661 gcccatgtgg tggcaatcgg tccggtctcg atgaactcct tgagccggga agaacaggaa
      721 agaatagaag ccatggacct gggcgaactc ttgctggcaa gccgcgacag ttcggtctac
      781 tgtccccacg aaggtctctc ggagcaaatg gaagccacca tcctgcaagc cctcaaggac
      841 ggcgacagcc tggggggagt ggtgcgggtc atggtcgacg ggctaccgcc cggtctgggc
      901 tcttcctttc aatgggatag aaaactggac gggcaactgg cccaatcgct catgagtata
      961 cagtcggcca aagccgtgga aatcggcacg ggagtcgaga atgccgccct gccgggctcc
     1021 cttgtacatg acgccatgca cagagacgaa agcggcaaga gcccctgtta ctacgtaggc
     1081 agaaaaacca accgctgcgg cggtctggag ggcggcatga ccaacggcga gcgcctgatc
     1141 ctgcatgtat atttcaagcc cattcccacc atgaagaagg gtctgccctc cctcagcttc
     1201 ccagctttca cggacagctt cgcccactac gagcgctcag acgtttgcgc cataccggcc
     1261 gcttctgttg ttgccggggc aatggtatcc ttgaccttgg ccaatgcatt ccttgacaaa
     1321 tttgctgccg acactatggt tgatctgaaa gagtcggtgg aaacatatag acaatatgtc
     1381 cgcaatctca cgaaaggtaa ggtagataat gcgccataaa gccaggcatc gcggtccggt
     1441 catcaccatg aactggaata ccacactgga tgttaaaacc agagtagcgg tcggtgccgg
     1501 cgccagacag aagctgtcct cgatattagc ccaggtaggc gccggtcgac gggttctaat
     1561 tgtttgccag cccaccacgg cggtgcactg gctacgagac attctcgaca ccctgcccac
     1621 ggaagatttt caagttacaa ccctggaagt gccggacggt gagcagtgta agaccacaga
     1681 atggctcctg cgcatctggg aacacctgga aggtcgtggt ttcgaccggc aagacaccgt
     1741 ggtggctctg ggaggcggcg ccgtcttaga cctggccggt tttgcctgtt caacatatat
     1801 gcgcggcttg aacctggtcc tcatccccac ctccctgctt gcccaggtag acgccgccat
     1861 cggcggaaaa aatgccatca acctgcccac cggcaaaaat ctcgccggta ctttcttctt
     1921 ccccaaggcc gtgctggccg accaggaagt gctgggcacc ttgcccgggc gacaattgac
     1981 ggcgggactg gctgaaatca tcaaatatgc cctcattgaa gaaacagtgg ccaaaggcac
     2041 cgactacgag aagggaccaa agccccttct ggagttggta gaagagatgg tgaaggaccc
     2101 ggtggagtat gacgacccga ccctctccgg cattatcacc aattgtataa agatgaaact
     2161 ttatgtagtt caccgcgacc cacacgaaac cggcatccgc cgcagtctca atcttggtca
     2221 taccttaggc catgccctgg aagcccagag cgaattcgag cttaaccacg gagaggctgt
     2281 ggccattggt ctctatcatg ccactaaata tagtgtaggt cagaagaaac tggagaaaga
     2341 cagcctggag agggtgaagg aagttttgat gcgagcggaa ctgccttacg aattgcccaa
     2401 aggcctttct aaagatcgcc tggtgcagca gatgaattcc gacaagaaac gctccggcga
     2461 aaaaattcgc ctggttatcc cccagacccg tctgggag
//
LOCUS       DAOQNZ010000545         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24450_length_2498_cov_3.128530, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000545 DAOQNZ010000000
VERSION     DAOQNZ010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24450_length_2498_cov_3.128530"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKW73_10280"
     CDS             complement(<1..427)
                     /locus_tag="PKW73_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNA73717.1"
                     /translation="MSAELSTLHDATLERLPPQSLEAEQAVLGALLVSGDGVSRVVDS
                     LEPEFFYRKAHQVIYAAMLDLYDANEPIDIVTVSQFLKDEGKLDMVGGRQYITDLSLS
                     VATTANLEYYAKTVQEKAVLRNLIKAGSEIVAHCYEETDA"
     gene            complement(569..2416)
                     /gene="acsA"
                     /locus_tag="PKW73_10285"
     CDS             complement(569..2416)
                     /gene="acsA"
                     /locus_tag="PKW73_10285"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNA73718.1"
                     /translation="MKLAQETPSKKSDSPETKKKDGKPGEATMTDAHKGPKKTGRKEL
                     IKKFPVKCNLEDYATTYDTFTWEAARSEISYFQDGRVNAAYNAVDRHLVSGRKNKVAL
                     YSVDAAGAVQKFTFQEIHDAACRMGNALKSLGVKKGDRVFVFLPRVPELYVATIAIAR
                     LGAIAGPLFSAFGPDALRDRLQDSEAKIVITNPALKEKLDGIKKDLPDLEHVIVVGAD
                     CKKLAKGELSYEELVKGQSNQLECEKTDPEDPLYLLYTSGTTGKPKGVVHVHADIVGH
                     HMTTKWVLDLRDDDIYWCTADPGWVTGTVYGIFGPWSNGASVVSYEGRFDADSWYKVI
                     DDLKVTVWYTAPTALRMLMKAGDDVVYEHDLSTLRHILSVGEPLNPEVVRWGMKVYGL
                     PIHDNWWQTETGMQLIANLPCNAIRPGSMGKPLPGVYAAVVDENGEEVEAGELGKLVV
                     KPGWPSMLRTIWRNEPKFQEYFKIPGWYFSGDNAWKDEDGYFWFVGRADDVINTSGHR
                     VGPFEVESALVEHKAVAEAGVIGKPDKERGEIIKAFVVLANGYTASDELLNDIKEFTK
                     KQLAAHAYPREIEVKESLPKTRSGKIMRRLLKAWELGLPTGDTSSLEDD"
BASE COUNT          466 a          697 c          695 g          640 t
ORIGIN      
        1 cggcatcggt ctcttcgtag caatgggcga caatttccga accagccttg atcaaattac
       61 gcaaaaccgc cttctcttgc acggttttag cgtaatactc caggttggcc gtggtggcca
      121 cggaaagcga caggtcggta atgtactggc gcccgccgac catatctagt tttccctcgt
      181 ctttgaggaa ctgggatacg gtgacgatgt ctatgggttc gttggcgtcg tagaggtcaa
      241 gcatggctgc gtagatgacc tggtgagctt tacggtagaa aaactcaggc tcaaggctgt
      301 ctacgactct ggaaacgccg tcgcctgata ccagaagggc gcccagcacg gcttgctccg
      361 cttccagtga ctggggcggc aacctttcca gggtggcatc gtgcaaagtt gataattcgg
      421 cgctcatatt tacttagacg cttttttctg cttccaggtt caatttcaat cagttcagtt
      481 tagccgagcg agccgttccc tgcactcttc agcgccgatt attaaggaag gtccccgcct
      541 tcatcgaagg cggagacctt tgcttggatc agtcgtcttc cagggaagag gtgtcgccgg
      601 tgggcagacc aagttcccag gctttcaaca gccggcgcat gattttgccg gagcgggtct
      661 tgggcagact ttcttttacc tcaatttcgc gcggataggc atgggccgcc aattgcttct
      721 tggtgaattc tttgatgtcg ttcaagagtt catcggaagc cgtgtatccg ttggccagca
      781 ccacaaaggc tttgataatc tcgcctcttt ccttgtcggg cttgccgatt actccggcct
      841 cggccactgc cttatgctcg accagggctg attccacttc aaagggaccg acccggtgtc
      901 cgcttgtgtt gatcacatcg tcggcccggc cgacgaacca gaaataaccg tcttcatctt
      961 tccaggcgtt gtcccctgag aaataccagc cgggtatctt gaagtattcc tggaatttcg
     1021 gctcattacg ccagatggtg cgcagcatgc tgggccaacc cggcttgacc accagtttac
     1081 ccagttcgcc cgcttccact tcttcgccgt tttcgtccac cacggccgca tagaccccgg
     1141 gcaagggttt gcccatggag cccggtctga tggcattgca aggcaaattg gcaatcagtt
     1201 gcatgccggt ttcagtctgc caccagttgt cgtggatggg cagtccgtag actttcatgc
     1261 cccagcgcac cacttccgga ttgagcggtt cgcccacaga caatatgtgg cgcagagtgg
     1321 agaggtcatg ttcgtaaacc acatcgtcgc cggctttcat gagcatacgc agggccgtgg
     1381 gcgccgtgta ccagaccgtc actttcaggt cgtctatgac tttgtaccag ctatcggcat
     1441 caaaacgtcc ctcatagctg actacggaag cgccgttcga ccagggtccg aagataccat
     1501 atacggtgcc ggtcacccaa ccgggatcgg ccgtgcacca gtaaatgtcg tcgtcacgca
     1561 ggtccagtac ccacttggtg gtcatgtggt gcccgactat gtcggcatgc acgtgtacca
     1621 ctcccttcgg cttgccggtg gtgccggagg tgtagagcag gtagagcgga tcttccgggt
     1681 ccgtcttctc gcattccagc tggttggatt gccctttcac cagctcctcg taggacagct
     1741 cgccttttgc cagtttcttg caatcggctc cgacaacaat cacatgttca aggtcgggca
     1801 ggtctttctt gataccgtcg agcttttctt tcagagccgg gttggttatt acaattttgg
     1861 cctcggagtc ttgcagcctg tctctgaggg catccggtcc aaaggctgag aagagtggac
     1921 cggcaatggc gcccagtctg gcgatggcga tggtggccac ataaagctca ggcacgcgcg
     1981 gcaggaaaac aaagacgcga tcgcctttct tcactcctag agatttgagg gcgttgccca
     2041 tgcggcaggc ggcgtcatgg atctcctgga aggtgaactt ttgaacggca ccggcggcgt
     2101 ccacgctgta gagtgctacc ttgttctttc ggccgctcac caggtgtctg tccacggcat
     2161 tgtaggcggc attgacgcgc ccgtcctgaa agtaagagat ctcggagcgg gccgcttccc
     2221 aggtgaaggt atcgtaggta gtggcatagt cttcaagatt gcatttgacc gggaacttct
     2281 tgatgagctc tttgcgcccg gttttctttg gacctttgtg ggcatcggtc atggtcgcct
     2341 caccgggctt gccgtccttc ttctttgttt cagggctgtc gctcttcttg ctcggagttt
     2401 cttgggcgag cttcatataa ttcctcccgg cttattgcat tgacctggct gtggttagag
     2461 atgcctttga gtctgctgcc ttgatggcat caggcagc
//
LOCUS       DAOQNZ010000546         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24498_length_2495_cov_3.189344, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000546 DAOQNZ010000000
VERSION     DAOQNZ010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24498_length_2495_cov_3.189344"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKW73_10290"
     CDS             complement(<1..355)
                     /locus_tag="PKW73_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73719.1"
                     /translation="MGKKTDKIHDGIDNLSKKVDYVSDRLFWIALSLALVTFVVVFSL
                     VQIIAILPVITLVHTAWAGLALALILGGFVYFIAARPMDVVDTASWSLWLAKLVLKMA
                     RKQGDAETASQPPVQN"
     gene            complement(467..1144)
                     /locus_tag="PKW73_10295"
     CDS             complement(467..1144)
                     /locus_tag="PKW73_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HNA73720.1"
                     /translation="MNLSKTNTRTRARASEDSSTLMAKVMMLLSGSMIISAIGSYMGQ
                     GITSGWVLFFLVILLFAGIFGVAAMAARSAALGVAGLAIWTFINGLVMGPAVNMYVQV
                     LGGGVVTMSFLGTAAVMAALGGYGALSGRDFSAMGRWLFLGLVGLIIVGVIGIFIGMG
                     HTFNLLYAIGGMVIFAGYFIFDFWRIANSENSDYNAVMYTMQVYLDFCNFWFSLMRFL
                     TEVMGDD"
     gene            complement(1256..1573)
                     /locus_tag="PKW73_10300"
     CDS             complement(1256..1573)
                     /locus_tag="PKW73_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73721.1"
                     /translation="MNNPNDGNNPLDNDPLLVGVTKGPTITAGSKIKVATEKLKPFFD
                     FHKRSTDKEDVIVEVLQMGMGTTDLGAFALVVIEVRLVDNDDPIDGLEMCVGFGDLVT
                     IKS"
     gene            complement(1679..2239)
                     /locus_tag="PKW73_10305"
     CDS             complement(1679..2239)
                     /locus_tag="PKW73_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73722.1"
                     /translation="MLSDSLTTGLIAVGLIILVIIAYHDEKRRTQRRRHNQLVLAETI
                     SIQKHLSLYVIPALEFLAEGITQPAHGGFPALVNASAAQVYLKEAFHSCRCAAVASIS
                     MNVVLFDEGLAKEQAQALLPLCRVLLKAAELHLAGYADAQLSMGEEDWQEIHVEALEA
                     AVVSLRCALQECVEPASADYSVLDQA"
BASE COUNT          654 a          698 c          610 g          533 t
ORIGIN      
        1 cgttttgcac aggcggttgc gatgcagtct ccgcatcgcc ttgcttccgc gccattttaa
       61 gtacaagctt tgccagccac aaagaccagg aagcggtatc aaccacatcc ataggtcttg
      121 ctgcaatgaa atagacaaag ccgccgagga taagcgccag tgccagtccg gcccaggccg
      181 tatgtaccaa tgtaatcaca ggcaagattg caataatctg caccagcgaa aacaccacta
      241 caaaggtcac aagagccaaa ctgagggcta tccagaaaag tcggtcggac acatagtcga
      301 cctttttcga taagttatcg ataccgtcgt gaattttgtc ggtctttttt cccatactga
      361 atttccgcga ggttgcttga aaagctggtc ccccctatga agaaggaacc agcttgttaa
      421 tgattacttc cctgacgaag cagtattgat gctagccctc agcagatcag tcgtcaccca
      481 tcacttcggt caggaagcgc atcaagctaa accagaagtt gcagaagtcc agatagacct
      541 gcatggtgta catgacggcg ttgtagtctg aattttcaga gttggcgatg cgccagaagt
      601 caaagataaa gtaaccggca aaaatgacca taccgccaat tgcatacaac aaattgaaag
      661 tatgacccat tccaatgaaa atgccgatca cgccaacgat gatcaagccc accagtccaa
      721 ggaacagcca gcggcccatg gcgctgaagt cacgaccgga aagagcaccg taacccccga
      781 gcgccgccat aactgctgcc gtacccagga aggacatggt caccacaccg ccgcccagaa
      841 cctgcacata catgttgacg gcggggccca tgaccagacc gttgataaag gtccagattg
      901 ccagaccagc cacccccagg gcagcagatc tggccgccat ggcagcgaca ccgaagatac
      961 cggcaaagag caagatcacc aggaagaaga gtacccaacc ggaggtgatg ccctgcccca
     1021 tatagctacc gatagcgctg atgatcatgc taccggaaag caacatcatc acttttgcca
     1081 tcaaagtgct ggagtcttcg cttgcgcgag cacgagtgcg tgtgtttgtt ttagataagt
     1141 tcatgagaat tcctcattgc taagttgatt gaatggttgt tgcaaccggg gaaggaggga
     1201 cgctttgaga gccgccctcc acctcggtta agaagcatcc ctgccgaaac agtgcttaag
     1261 acttgatggt gaccagatca ccgaagccga cgcacatttc cagaccgtcg atggggtcgt
     1321 cgttatccac cagacgcact tcgatcacta ccagggcaaa cgcgcccagg tcggtagtgc
     1381 ccatgcccat ctgcaatact tccacaatta catcttcctt atcggtgctg cgcttgtgaa
     1441 agtcgaagaa gggcttgagc ttttccgtag ccaccttgat tttgctgccg gcagttatgg
     1501 tcggaccttt tgtcacgccg accaaaagtg gatcgttgtc taacgggttg ttaccatcgt
     1561 ttgggttgtt cataagtttt cccttgtttg ggtttgattt agttggatga aacgaaccac
     1621 agtgcacaaa ctattgcgca ctgctgaaag aaccggagtc agacggcaaa ccgaagtttt
     1681 aagcctgatc cagcacggaa tagtctgccg aagcaggctc cacgcattcc tgaagcgcac
     1741 agcgcaagct gactacagcc gcctccagag cttccacgtg aatttcctgc cagtcctcct
     1801 cgcccatact gagctgggca tcggcataac cggccaagtg caactccgcc gccttgagca
     1861 aaactctgca cagcggcaag agagcctgag cctgctcctt agccagccct tcatcgaaaa
     1921 gcaccacatt catgcttatg gaagcgaccg ccgcacagcg acagctatga aaagcttcct
     1981 ttagatacac ctgtgccgcc gaggcattca caagcgccgg aaagccgcca tgtgccggtt
     2041 gtgttatgcc ttccgccaga aactccagcg ccggtatgac atacaggctc agatgcttct
     2101 gtattgaaat ggtctcagcc agcacaagtt gattgtgccg ccttctttga gtgcgtcgct
     2161 tctcgtcgtg gtaggcgatg attacaagaa tgatcaaacc cacggcgatt aagcctgtgg
     2221 tcagactatc actgagcata tggactccgt ttactatttt tatgtggagc aggtcgaagc
     2281 aaagtccgac ctgccgattc aagcagcagg gctaacaatc agcccaacaa gccatccttc
     2341 tccaccacga tgggcttacc aaggttgcct tcggcttcaa gcaacatggc ggtgactggg
     2401 tccacttctt cttcgacaac agcaaccgcc ggagcagtca ctgaagccgt ggcgccggaa
     2461 ggcaataaac tatccagtaa ggcagccaga tcgga
//
LOCUS       DAOQNZ010000547         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24508_length_2494_cov_3.924149, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000547 DAOQNZ010000000
VERSION     DAOQNZ010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24508_length_2494_cov_3.924149"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKW73_10310"
     CDS             <1..415
                     /locus_tag="PKW73_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA73723.1"
                     /translation="FFLGPTIFDHAKPEMTIVKEEIFGPVLSVVRARTLEDAIEVGKK
                     CPYGNGAVIYTNSGRSAREFKRHFNAGMIGVNVGVPAPMAWFPFTGWNNSFFGDLHIQ
                     GSEGIQFYTQQKMTMTRWFEPKNASFQDPIWKPKN"
     gene            650..1369
                     /locus_tag="PKW73_10315"
     CDS             650..1369
                     /locus_tag="PKW73_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73724.1"
                     /translation="MGIKLMYWHMFLNPGLTVNELLLGQRFPSRLYVCKACELPLADR
                     TFVHCPECDSFLDGRLWSYGNAFGHWLGLFCPTCGGEIPCLRNLTTAAILAVSAPILW
                     LPQKLCKDRLLPSQKLRIARAQDKYLTTPDIKQRKPVNYWRMGLLWGLSMDVIFSFFL
                     GARSINWIHGGWTELFVIYGFLLLKGLLIWLPAGAVVAVTCKLMMDRKGDVNLHLTYD
                     ADGQVVPLKCDIDEEAQDGKV"
     gene            complement(1443..2348)
                     /locus_tag="PKW73_10320"
     CDS             complement(1443..2348)
                     /locus_tag="PKW73_10320"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNA73725.1"
                     /translation="MDFSAWTQPSALGTFTVWFVLSYLYAGLGITLGYHRLLTHKGLK
                     VPKWVMYFFVSGGYLSLMGAPISWVATHRLHHQKSDLPGDPHSPRDGFKHALYEWMMH
                     MEEKQDAAEVARQTPDLMTDPVLCALGTSHSARQAYLCLAANIVWRLAILAFFGPVAF
                     TANFLAGFIVFWSPQFVNTFCHLKEHGYRNFVSRDDSRNVWWVAALACGEGWHNNHHA
                     FPKSARHGMMWWEFDITWCTIWLLEKVGLAKDVVRPNPTAMAASRIKEAAGGRVVEPV
                     EELTAAVSKVEAPDMVAVPEPSAVN"
BASE COUNT          629 a          658 c          652 g          555 t
ORIGIN      
        1 cttcttcctg ggcccgacta ttttcgacca cgccaaaccc gaaatgacca tcgttaaaga
       61 agagatcttc ggaccggtac tgtcggtggt gcgagccagg actctcgaag acgccatcga
      121 agtaggtaaa aagtgccctt acggaaatgg tgccgtcatc tatacaaatt cgggtcgctc
      181 cgccagagag ttcaagcgcc acttcaatgc cggcatgatc ggcgtcaacg tgggcgtacc
      241 ggcacccatg gcctggttcc ccttcacggg ttggaacaat tccttcttcg gcgatttgca
      301 cattcaaggc tcggaaggaa ttcaattcta cacccagcaa aaaatgacca tgacccgctg
      361 gttcgaacct aagaatgcct ccttccagga tccaatctgg aagccgaaga actgaggacg
      421 gaagtattcc agcaagcttg cgattggggg aaccgagaag ttcccccaat ctgctaaccg
      481 ggttctcacc gagccggggc attaaccagg acaagcatcc agttacgtcc cactctgatt
      541 cgggaataga aaaccagaag ctatcgagga tttcccagtg ttagtggact tcgaatgccc
      601 taaatgcaag ggacatatct gcgaaatttg ggcgcttaac ggctcgaact tgggcatcaa
      661 actgatgtac tggcatatgt tcctcaaccc cggtctgacc gtaaacgaat tactactggg
      721 gcaacgcttt cccagtcggc tctatgtgtg caaggcctgc gaactgccgc tggccgacag
      781 aacctttgtt cattgcccag aatgcgacag ctttcttgac ggtcgactct ggagttacgg
      841 caatgccttc ggtcactggc tgggattatt ttgccccacc tgtggcggcg agataccttg
      901 cttgagaaat ctcactactg ccgccattct tgccgtcagt gcgccgattt tgtggcttcc
      961 gcagaaatta tgcaaagaca gattgctacc atcgcaaaaa ctgcgtattg ccagggccca
     1021 ggacaagtat ctaaccacac ctgatattaa gcagcgaaag cccgtcaatt actggcgtat
     1081 gggtttgcta tggggtctgt ccatggatgt gatcttctcc ttcttcctgg gtgccagaag
     1141 catcaactgg atacacggcg gctggaccga gctttttgtt atttatggct ttctgctgct
     1201 caaaggatta ttgatctggc ttccggccgg agcagtcgtt gccgttacct gcaagctcat
     1261 gatggacaga aaaggcgatg taaatctgca ccttacttat gatgccgacg gacaggtggt
     1321 gcccttgaag tgcgacatcg acgaagaagc acaggacggc aaagtataga ggcgacaacc
     1381 gcattcaaac aaaaaactcg ccggcttgcc gacgagtttc tcgtttaagt ttatctttca
     1441 gctcaattga ctgcgctggg ctcgggcact gccaccatat caggtgcttc gaccttggag
     1501 acggcagcgg tgagttcttc caccggttcc actactctgc caccggccgc ttctttaata
     1561 cgggaagcag ccatggctgt aggattgggt ctgactacat ctttagccag gccgactttc
     1621 tcgagcaacc agatggtgca ccaggtgatg tcgaattccc accacatcat gccgtgtctg
     1681 gccgacttgg gaaaggcatg gtggttgttg tgccagcctt cgccgcaggc aagagcagcc
     1741 acccaccaga cgttgcggct gtcgtcacga gaaacgaagt tgcggtagcc gtgttctttc
     1801 aaatggcaga aggtgttgac gaactggggg ctccagaaga cgatgaagcc ggccaggaag
     1861 ttggcggtga aagccaccgg accaaaaaac gccaggatag ccagacgcca gacaatgttg
     1921 gcagccaaac acaagtaagc ttgacgagca gagtggctgg taccgagagc acacaggaca
     1981 gggtcggtca taagatcggg agtctgcctg gcaacttcgg cagcatcctg cttctcttcc
     2041 atgtgcatca tccactcata aagagcgtgc ttgaaaccat cacgaggaga gtgcggatcg
     2101 cctggaaggt cggatttctg gtggtgcagt ctgtgtgtgg ccacccagga aatcggcgcg
     2161 cccatcaagg acaaatagcc accggagaca aagaagtaca ttacccattt gggaactttc
     2221 aggcctttat gcgtcaaaag tctgtgataa ccgagcgtta tgcctaaacc ggcatagagg
     2281 tatgagagca cgaaccagac ggtgaaagta ccaagcgccg acggctgtgt ccaagctgaa
     2341 aaatccaaaa tcaagccccc atgtcttctt gcggaaatga ccgcggttca atagtcacca
     2401 tcgccatatg aaatgcgatt cacaatcaaa cagaaaggct aactgcgtta aagaaagcca
     2461 gactctgcgg ataatctcat ttatatcacg gacc
//
LOCUS       DAOQNZ010000548         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24558_length_2491_cov_3.634236, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000548 DAOQNZ010000000
VERSION     DAOQNZ010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24558_length_2491_cov_3.634236"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKW73_10325"
     CDS             <1..477
                     /locus_tag="PKW73_10325"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNA73726.1"
                     /translation="DWRNAATARFLQLVQTPVTDSELLYHVTRLFPKPVEYGRTELHA
                     STVDQLRNDQGRLDAKLVADLFGLTLTDVAANVGISRQALSKTPDSLSVQGALNQFER
                     IARSLLTVTGTEKGLKTWLNAPNKRFDQHTPLEVIKLGKAALLADWVDDARLGSPQ"
     gene            565..1071
                     /locus_tag="PKW73_10330"
     CDS             565..1071
                     /locus_tag="PKW73_10330"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNA73727.1"
                     /translation="MSALVGGANRRIQPLFDLGPGRSGQRFTPVGGPRALYVAEDPYT
                     SYLESTGMFDSVADLAEKLADTQSTLQFRVQLEEVLDLTVADTMLELGTNQEELLASW
                     EWQMATGARVPTQDLGAAAFASKRFQAIRFTSAKRADHANLVIFTDRVVAPAFVECTD
                     SNFPDRLP"
     gene            complement(1123..>2491)
                     /locus_tag="PKW73_10335"
     CDS             complement(1123..>2491)
                     /locus_tag="PKW73_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA73728.1"
                     /translation="PVGRLKSVVCSMLVLLACQAPVFAQESSEVVNAGGERQTGLAVN
                     RPIADKWALIVGIGNFADSKIPKLKYATKDARDFYNYLVKDAHFKPDHVRILLDETAT
                     QRRIVSELGSRGLPRLVRPDDLVVIFFSTHGSPSQMDNQGSNFLVAYDSDPDDLYATG
                     VEMQKILNQIKERIGSDRVLLVMDACHSGSISKNAKGLKRSANFDAKALAEGSGQLVI
                     CSSLPDEQSWESKRYENGVFTKNLIDGLRSNPSATISSVFEKMSDLVAAEVKEDYPGA
                     KQTPALHSKWTGNDLVISALPSSPRIIPETVVLDLEPDSSALIGRPPTFVAKPKKGAT
                     PLNKPSADDAQKSVLVITKDYFGKEEDPRKAYQEACAQQAAHFNEPEYYYRKARVLIQ
                     LGNYSKADQELKGLLVDNPNSSAYHLAKAYCNHKLGNKFAAQDEIGMAKFHDPSLPRE
                     IVFGD"
BASE COUNT          622 a          628 c          661 g          580 t
ORIGIN      
        1 gactggagga atgctgccac tgcacgattc ctccaattag tgcagactcc ggtaactgat
       61 tctgaattgc tatatcatgt gacacgtctc tttcccaaac cggtggaata cggtcgaacg
      121 gaactacatg caagtactgt cgatcagctt cgaaacgatc aaggtagact cgatgctaag
      181 ctggtggctg acttgtttgg tttgacctta actgacgtgg cagcaaatgt gggaatttca
      241 cggcaggcgt taagtaaaac accagactcg ttgtcagtcc agggcgcact caaccagttc
      301 gaacgaattg cacgcagcct gctaacggta accggtacag agaaaggact aaagacctgg
      361 ctgaatgcgc caaacaaacg gtttgatcaa catacaccac tggaagtaat aaagctaggc
      421 aaagccgcac tacttgcaga ttgggtagac gatgcccgac tggggagtcc gcaatagatg
      481 catgatgcac tcgggatagc ccgggccctg aaaaggatag ccccggtaaa gtacccagag
      541 cgcaatctat tccgctgtgt cgagatgtcg gcactggttg gcggagccaa tagacgcatc
      601 caacctcttt tcgatctggg accagggaga tccgggcagc gcttcacgcc ggttggcgga
      661 ccgcgagccc tttatgttgc agaggaccct tacacatcgt atcttgagtc gaccggcatg
      721 ttcgattcgg tagccgacct agcagaaaaa ctcgcagata cacaaagcac cttgcagttt
      781 cgtgtgcagc tagaagaagt tctagaccta accgtggcag acacgatgct tgaacttggt
      841 accaatcagg aagaactgct agcctcgtgg gaatggcaga tggcaacagg tgctagagtt
      901 ccaacccaag atttgggagc ggcggcattc gccagtaagc gctttcaggc aatccggttc
      961 acgtccgcga agcgtgccga ccatgctaat ttagtcattt tcactgatcg tgtggtggca
     1021 ccggcatttg ttgagtgtac cgacagcaac tttccagatc gtcttccata aggactgtga
     1081 accgctacca gattgttcaa ttccgccgac caaagtaagc aatcaatcgc cgaaaacgat
     1141 ttctctgggt aggctggggt catggaactt agccatgccg atttcatctt gagcggcgaa
     1201 cttattacca agcttgtgat tgcaataggc ttttgccagg tgataggcgg aactgttggg
     1261 attatcaacc agcaagccct tgagctcctg gtcggctttg gaatagttac ccaactgaat
     1321 caagactctg gccttgcgat aataatattc cggctcgtta aaatgggccg cctgctgagc
     1381 gcaagcctcc tgataggcct tgcgcggatc ttcttcttta ccaaagtagt ctttggtaat
     1441 cacaagaaca cttttctggg catcatcggc ggaaggctta ttgagaggtg tagcgccttt
     1501 cttgggctta gcgacaaagg tcggtggccg accaatcaga gccgagctat ccggttccag
     1561 atcaagcact acggtttcgg gaataatgcg gggcgaggac ggcaaggccg agatgaccag
     1621 atcgttgccg gtccattttg aatgtagagc aggagtttgc ttggcgccgg gataatcttc
     1681 ttttacctcg gcggctacca ggtcggacat tttctcgaac acactggata tggtggcact
     1741 gggattactc ctcaagccat caataaggtt ctttgtgaaa acaccatttt catatcgttt
     1801 cgattcccag gactgctcat caggcaaact ggaacagatg accagttggc cggagccttc
     1861 agccagggcc ttagcgtcga aattggcgct gcgtttcaaa cctttggcat ttttagagat
     1921 gctaccgctg tggcaagcat ccataacgag aagcacccgg tctgagccga tgcgctcttt
     1981 gatttgattg aggatctttt gcatctctac accggtggcg taaagatcgt cgggatcgga
     2041 atcataggca accaggaaat tgctaccctg gttatccatc tgagagggag agccgtgggt
     2101 ggaaaagaag atcaccacca ggtcatcggg tcggaccagg cgcggcagac cacgactgcc
     2161 cagttcggaa acgatacgtc tctgggtggc ggtttcgtct aacagaatac gcacatgatc
     2221 cggcttaaaa tgagcgtctt tgaccagata gttgtaaaag tcgcgggcat ctttggtggc
     2281 atacttaagt ttgggaattt tgctgtcggc gaaattgcct atccccacaa tcaaagccca
     2341 tttatcagca atcggtcgat taaccgccag accggtctga cgctcgccgc cggcgttaac
     2401 gacttcgcta ctctcctggg caaaaaccgg tgcctgacat gccagaagaa ccagcatgct
     2461 gcaaaccaca gacttgagcc ggcccaccgg a
//
LOCUS       DAOQNZ010000549         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24623_length_2488_cov_3.289355, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000549 DAOQNZ010000000
VERSION     DAOQNZ010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24623_length_2488_cov_3.289355"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PKW73_10340"
     CDS             <1..720
                     /locus_tag="PKW73_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73729.1"
                     /translation="GIMHENLNRPGALFLTFDGGNYAGLTTLKQVLNTSEFCLEPTDT
                     VELPPAGDSPPKNLANATIPPFIDNHPPFGQVMRVAIYDWIRRYRDQPNVQSLLDVFD
                     DSFVADTNPGGSYYTMLANGTLIQRTFAVGGVNRLAIMPVAHRQYRAVSGIALDTDTY
                     DFDLIVKDFVNRRGRIEGGDHGGEPYPWTGGTVKVIAQPREIDDEVTMVYTYPPLTGD
                     TKRPGWSNSLAVCSEIRVRKR"
     gene            complement(725..1168)
                     /locus_tag="PKW73_10345"
     CDS             complement(725..1168)
                     /locus_tag="PKW73_10345"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNA73730.1"
                     /translation="MSKKSAPKKYLWNALSAIGGKCLSVSDGHAVFELPLTEEVMQPT
                     GVYHAGMIVTLADEAASAAAYGGPIDARDIVPEKKFPYSVQLSVNLLTNDPVGPIRAE
                     ATVVRRGRLTVVDTEVTNQKGETMALMRSTHMMVDASRVGPHLKK"
     gene            complement(1180..2334)
                     /locus_tag="PKW73_10350"
     CDS             complement(1180..2334)
                     /locus_tag="PKW73_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73731.1"
                     /translation="MDALAYRFLLPVIAAAVLAVSDLHTLPAFAQGDTARAYSLGETP
                     RPQPPQQSNLARAVRAHKLFKNLSISTFTRGRQAVICVYGYNGANYRLDAVQAARAVT
                     AAYSGQFLTTAVRYYEKEQGYKEVLLTSRDITSLEAGTIKAGELALQAPEVNIGSYDG
                     PAVVFEKYLAAAESLIEKGSYFEAEQIVDSLGPAPGGAAADRFARDMLQLSQGFDTYG
                     DLYRAARILEKVVDERKNAGTLLSADADLTVERLIDLYLADKRYSDAENLLKELVARP
                     EQGQARTNNLERLAVVQLRLGKNEEALSALNEVLKAREAGPVAALARTLENIGDAYRA
                     LGRKGDAQASYKRARAVYDKAVVATKREQRIDYQVYAGRVKQLDEKLKLQ"
BASE COUNT          568 a          740 c          632 g          548 t
ORIGIN      
        1 ggcatcatgc acgaaaatct caatcgtccc ggcgcccttt ttcttacctt tgacggcggc
       61 aactatgccg gtctcacgac cttaaagcag gtcttgaaca caagcgagtt ttgcctggaa
      121 cccaccgata cagtggaact tccgcctgcc ggcgatagcc ccccgaaaaa cctggctaat
      181 gccaccatcc cgcccttcat cgacaaccac cctccctttg ggcaagtgat gcgagtggca
      241 atttacgatt ggatcagaag ataccgggac cagcccaatg tgcaatcctt gctcgatgtt
      301 ttcgacgaca gctttgtggc cgacaccaat cccggcggct cttactacac tatgctggcc
      361 aacggcaccc tcattcagag aacctttgcc gtgggcggag tgaaccgact ggccattatg
      421 cccgttgccc accggcaata ccgcgccgtc agtggtattg ccctcgatac cgacacctac
      481 gacttcgatc tgattgtcaa agacttcgtc aatcgccgcg gccgcatcga aggtggtgac
      541 cacggcggcg aaccctaccc ctggaccgga ggcaccgtca aggtaatcgc ccagcccagg
      601 gaaatcgatg acgaagtcac tatggtctat acctatccac ccctgaccgg cgacacgaaa
      661 agaccgggct ggtccaatag cctggcagtt tgctccgaga ttagagtgcg aaaacgttga
      721 gtccctattt cttcaaatga ggaccgactc tggaagcatc caccatcatg tgagtactgc
      781 gcatgagggc catggtctcg cccttctgat tagtcacttc cgtatcaacc acagtgaggc
      841 gaccgcggcg caccacagtg gcttcagcgc gaatgggacc aacaggatca ttggtaagaa
      901 gattgacgct caattgcacc gaatacggaa acttcttctc cggtacgata tcgcgggcat
      961 cgattggtcc tccgtaagcg gcggcggaag ccgcttcatc ggccagcgtc acaatcatgc
     1021 cggcatgata gacaccggtg ggttgcatca cttcctcagt caaaggcagt tcaaaaacgg
     1081 catgaccgtc actcaccgat aaacacttgc cgccaatggc actcaaggca ttccacaaat
     1141 actttttggg ggcggacttc ttgctcattt tttcgcctgt tactggagtt tcaatttctc
     1201 atccaactgt ttaacacgcc ctgcgtaaac ttgatagtca atacgctgtt cgcgtttggt
     1261 ggccaccacc gctttatcgt aaacggcccg ggctcgcttg taggaagcct gggcatcgcc
     1321 tttgcgacca agggcccggt aagcatcgcc gatattttcc agggtacggg caagagccgc
     1381 caccggtccg gcttcgcgcg ccttcagcac ctcattcaag gcgctgagag cctcttcgtt
     1441 ctttccgagg cgcagttgca caaccgccag acgctccaga ttattggtgc gggcctgacc
     1501 ttgctctggt ctggccacta gttccttgag caaattttcg gcatcgctat aacgcttgtc
     1561 ggccagatac aaatcaatta gccgctccac cgtcaaatcg gcatcggcac tgagaagcgt
     1621 accggcgttc ttacgttcat ctacaacttt ttcaagaatt ctggccgctc tatataaatc
     1681 accataggtg tcgaagccct gagaaagttg caacatatcg cgggcaaatc gatcggcagc
     1741 ggcgccacca ggggcaggac caagactgtc gacaatttgc tcggcttcaa aataggagcc
     1801 cttctctatc agtgactccg ctgccgccag atacttttca aacaccacag ccgggccgtc
     1861 atagctgcct atatttacct ccggggcttg caaagccagt tcaccagcct taatggtacc
     1921 ggcttccaga gaagtaatat cgcggctggt caggagcact tccttgtagc cctgctcttt
     1981 ctcgtagtaa cgcaccgcag tcgtcaaaaa ctgcccggag taagccgccg taacggctct
     2041 agccgcctgg acagcgtcga ggcggtagtt agcaccgtta taaccataga cacaaataac
     2101 tgcctggcga ccgcgcgtaa aggtactgat actgagattt ttgaaaagct tgtgggcgcg
     2161 aactgccctt gctaaattgc tttgctgcgg cggctgcggg cgaggcgtct ctccgaggct
     2221 gtaagcccgg gccgtgtcac cctgggcaaa ggccggcaaa gtgtgtaaat cagatacagc
     2281 caaaacagcg gcggcaatca caggcaataa gaaacgatag gcaagggcgt ccaaggttaa
     2341 cctccaggtg ttccccatat tctaatgcag ccccggcctg cccgcctctt tttggaggcg
     2401 ctctatttca gaacgtatgg ctgcgatccg atcgctggtc atggggtggg tggaaagtag
     2461 cgacatagcc agtttgccgg actcgccg
//
LOCUS       DAOQNZ010000550         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24633_length_2487_cov_4.525905, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000550 DAOQNZ010000000
VERSION     DAOQNZ010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24633_length_2487_cov_4.525905"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKW73_10355"
     CDS             <1..331
                     /locus_tag="PKW73_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73732.1"
                     /translation="GKDEDDGFRLDRRFVGTVVSIKPDNVVNIKLIGGDSESLKEQAK
                     GWKRLLTSDSTSAGYLFNPDLKQDAQTVKITYKANSVEIEQKCKDYQSAMFGLALLSA
                     MGYVVFL"
     gene            complement(379..1638)
                     /locus_tag="PKW73_10360"
     CDS             complement(379..1638)
                     /locus_tag="PKW73_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73733.1"
                     /translation="MAQSDDEKQPGSKKPPENLSLRKSRQVDQKQFQSMTDLAAKEQA
                     ATFEIIEEEAVLASKRVKPDLPNPHEKALEHYLQPPPDQFRRNRQEEGALPRDLLARD
                     LERACLTLLQSLGENVNLTHHGSEDEKRLALLRAVRAIVSDPTVTGPIATIAALTAGY
                     YHNSQTSLEPTLEDSESFAYFAADRFSLLEGKRLEGALLPDKQGFLEAGEEIVAGFAP
                     SQNFLLAFLFSCLPCATADLKNKIKPLENSYLVTFPGAAPLKVSPLSQGERCYGACDL
                     RGFDYLAILEKAYGQHLTEKLTEPLKPVIAADGAKAAADTAHCHLALNLLTGRAPLVM
                     AKPMNEKAAALWRKEIEDRLRQSRQTNSTMYCRRLSETGAPLDCALKFEMGQLTQYYF
                     DRLCRLRSDILPLSALFDGAQVFYQAG"
     gene            1752..>2487
                     /locus_tag="PKW73_10365"
     CDS             1752..>2487
                     /locus_tag="PKW73_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73734.1"
                     /translation="MKTGNPVLFIMIAGVLYLSYALYAQKGSMQTQRGPEMPGELAGD
                     SPAADEPALASLSVNVPKKLSEQARQQIAYLKQPIKVRPFSSHHNSLSHYAYLRILDR
                     SQEGDRVGEINRLKEALPKQSNQAFASLVLQILADGEKNDPKNGLKHALAAVDLIDRD
                     EKLVADKKMPPLALLVPFVYGARFALMLDDEAEACALAQESLTYCRQANDFALKDMEA
                     LYYSAEKQGADFALFNKTSESFIESLK"
BASE COUNT          540 a          621 c          700 g          626 t
ORIGIN      
        1 aggtaaagat gaggacgacg gattcaggtt agaccggcgt tttgttggca cggtggtaag
       61 catcaagcct gataacgtag ttaacatcaa actgattggc ggcgatagtg agtcgctcaa
      121 agagcaggct aagggttgga aacgactgct aacttcagat agtaccagcg ctggctactt
      181 attcaaccca gacctgaagc aagatgctca gacggtcaag attacctaca aagctaactc
      241 agtcgagata gaacaaaagt gtaaagacta ccagagtgct atgttcggcc tggcgcttct
      301 tagcgcaatg ggttatgtag tgtttctcta gagccctttt atggacaggt cttagtgaat
      361 tcaccagggt tttcagcctc accccgcctg gtagaaaacc tgggcgccgt cgaagagggc
      421 cgagagcggc agaatgtctg agcgcaggcg gcagagccta tcgaagtagt actgggtaag
      481 ttgtcccatt tcaaacttga gggcgcaatc cagaggcgcg cctgtttcgc tcaggcgcct
      541 gcagtacatg gttgaattag tctggcgcga ttgcctgagg cggtcttcta tttctttgcg
      601 ccagagtgcc gcggcttttt cgttcatcgg ctttgccatt accagcggtg cacgtcccgt
      661 caataggttg agagccaggt ggcagtgtgc cgtatcagcg gcggctttcg ccccgtccgc
      721 cgctattacc ggtttgagcg gctcagtcag cttctctgtc agatgctgcc cataggcctt
      781 ttctagaatg gccagatagt caaagcctct caggtcgcag gctccgtaac agcgctcgcc
      841 ctgggagagg gggctgactt tcaagggcgc cgcacccggg aaagtcacca ggtagctgtt
      901 ctccaaaggc ttgattttgt tttttaagtc tgccgtagca cagggcaggc agctaaagag
      961 aaaagcgaga aggaagtttt ggctgggtgc aaagccggct acgatttctt cgccggcctc
     1021 taagaaaccc tgcttgtccg gcaagagtgc cccttcaagg cgtttgcctt ccaaaagact
     1081 gaaccggtca gcggcaaaat aggcaaagct ctccgaatct tccagggtcg gttccagaga
     1141 agtttgactg ttatggtagt aacctgccgt cagggcggct atggtggcta tgggtccggt
     1201 taccgtaggg tctgaaacta ttgctcttac cgctctcagg agggccaggc gcttctcatc
     1261 ttcggagccg tgatgggtca agttgacgtt ctcgcccagc gactgtagca aggtcagaca
     1321 ggcccgctcc agatcgcggg ccagcaaatc tcttgggagg gcgccttcct cctgccggtt
     1381 tctgcgaaat tgatcgggtg gcggctgcag gtaatgctct agcgccttct cgtggggatt
     1441 gggtaagtca ggtttgacac gcttcgaggc cagcaccgcc tcctcttcaa tgatttcgaa
     1501 agtggcagcc tgctcttttg ccgccaggtc ggtcattgat tgaaactgct tctggtcaac
     1561 ctggcggctt ttgcgtaagc tgagattttc aggcggcttc tttgagcctg gctgcttttc
     1621 atcatcggat tgcgccatgt ttccactttc cttcaaggcg attagtctat tttctgataa
     1681 ccaggtaaaa tttgcagacg aggatagctc ctctttgctc tatctattct ttcatctcaa
     1741 gggggcgggc agtgaaaacg ggaaatcccg tgttgttcat tatgatagcg ggagtgctct
     1801 atttgagtta tgccctttat gcccagaaag ggtctatgca gacccagcgc ggtccggaga
     1861 tgccagggga acttgccggt gatagcccgg cggcggacga gcctgccctt gcttcgcttt
     1921 cggtgaatgt gcccaagaaa ctttcggagc aggccaggca gcaaatcgct tacttgaagc
     1981 aacccattaa ggtcaggccc ttttccagtc atcataattc tctcagtcac tatgcatatt
     2041 tgagaatatt ggaccgttcc caggaagggg accgagtcgg ggaaatcaat cgcttgaagg
     2101 aagccctgcc caagcagtcc aatcaagctt ttgcttctct cgttttacag attctcgctg
     2161 atggagagaa gaacgatcca aagaacggtc tcaagcatgc tctggctgcc gtggatctta
     2221 tcgaccgtga tgagaagctg gttgccgaca agaaaatgcc gccgctggcc ttactggtgc
     2281 cctttgttta tggtgctcgt tttgcactca tgctcgatga tgaggcggaa gcttgtgcct
     2341 tggcgcagga gtcgttaact tattgccgcc aggccaatga ctttgccttg aaagacatgg
     2401 aggctctcta ttacagcgct gagaagcagg gggcggattt tgccctcttc aacaagactt
     2461 ccgagagctt tattgagagt ctgaaag
//
LOCUS       DAOQNZ010000551         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24778_length_2480_cov_2.804948, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000551 DAOQNZ010000000
VERSION     DAOQNZ010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24778_length_2480_cov_2.804948"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKW73_10370"
     CDS             <1..210
                     /locus_tag="PKW73_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73735.1"
                     /translation="LGSFKVGLSSSLGIARVITEFEFLGLGVGELDKISSRYIDLTKE
                     QVDEAMQRYFHPERMVTVLSGSIVK"
     gene            304..966
                     /locus_tag="PKW73_10375"
     CDS             304..966
                     /locus_tag="PKW73_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73736.1"
                     /translation="MQSQAQVKLEVLSPALIAVPNTPDEVQQSADVQRLELLLGKIFN
                     DEIEKALKDEHHFQSLSEVGTHSPVHLPSSSLLDFTPFFEEAKLPVHEQTEIFFDGEA
                     ITQKNLVSIFEGDDDDHALAIDNLNQVIEAQRRELVTLRQALKTSRDDARILHLDLAD
                     KDDQLAALPELIAAPLLKELELEKAAHASTASHLVQAQGRLAKFDQSLWVKLAKIFGL
                     SA"
     gene            1083..1271
                     /locus_tag="PKW73_10380"
     CDS             1083..1271
                     /locus_tag="PKW73_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNA73737.1"
                     /translation="MVVTRDVSKGTVATQSLLAEKRLEKRLAPINAVSGISECVGRAF
                     KRARSKGQIILIEDLENP"
     gene            1284..>2480
                     /locus_tag="PKW73_10385"
     CDS             1284..>2480
                     /locus_tag="PKW73_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73738.1"
                     /translation="MNRRVGLIALLALSLGLGIPPATLALSGADVVAAIDKAKVLDAS
                     IRVNAQVTPEVIYISTYKNPKANDRDCKIEAVLLAKTVMELDNKIPRVEIRFYSQNAL
                     SRFKKISVSAGDVKAFAAGTMQEDQLLNSLVLAEEETKDPNVRVTSHLQENQYARRKR
                     EVSSRINKNVLEVSAGLDPTLEDVYLKAEALRLAEKAFEAVGDDIDEVSITFYDGSKE
                     LSNRNVTLKRAQQKPFSEALNSALKDLELKTVKAEKLAAEGLDVTTFEPSEGLYLKER
                     KELVGRLRALKEAGVGFSRDVLVDLVAIENGLTTLDEAVLRDKLAKLSAFIGKYEENL
                     KKAKEIKPAAAPKASAAAKTNDPVLSENVDQMKAPVLANPDGYLATMAGRLARRSPTG
                     NGEDHPN"
BASE COUNT          654 a          660 c          632 g          534 t
ORIGIN      
        1 cttggttctt tcaaagtagg actctcttcc tctcttggca tcgccagggt tattacagaa
       61 tttgaatttc ttggtctggg tgtcggtgaa ttagacaaga tatcttctcg ctatatagat
      121 ctcacgaaag agcaggtgga cgaagcaatg cagcgctatt tccatcccga gagaatggtc
      181 accgtcctca gtggcagcat tgtcaagtag ttaaatcgaa agattaactc tatacaatta
      241 tgtgcagctg agataacatt aaggccgcgg tgatgacaac cgtctatatc aaacgaggtc
      301 tcgatgcaaa gccaagctca agtaaagttg gaagtattgt cgccggccct gattgcggtg
      361 ccgaacaccc ccgatgaagt acaacagagc gccgacgtgc agaggctcga acttcttctg
      421 ggcaaaatct tcaacgatga aattgaaaaa gctctcaaag acgagcacca ttttcagtca
      481 ttaagcgaag taggcacaca tagccccgta catcttcctt cttcctcttt gctcgatttt
      541 acgcccttct tcgaagaagc aaaactgccg gtccacgagc agacagagat tttctttgat
      601 ggtgaggcaa tcactcagaa aaatctggtc agcatattcg agggtgacga cgacgatcac
      661 gcactggcga tcgacaatct caaccaggtc attgaagccc agcgccgcga gcttgtcacc
      721 ttgaggcagg ctctcaaaac cagccgggac gatgcccgaa ttttgcatct cgatctggcc
      781 gataaagacg atcagttggc cgctctgccc gaacttatcg ccgcccctct tctgaaagag
      841 ctggaactgg aaaaagcggc ccatgcctct acagccagtc atctggtgca agcccagggt
      901 cgcctggcca aattcgatca gagcctctgg gttaagttag cgaaaatttt cggactatcg
      961 gcgtgaaccc tgcatctaaa aagccaatag tcagatccct cgtcctgatc ggttgcatat
     1021 tggggcaggt ggctggtgct ttagcccagc ccgctccttc accaaaggat gaacaggttg
     1081 tcgtggttgt aaccagagac gtaagcaaag gtaccgtagc cacccagagc ctgctcgccg
     1141 aaaagcgcct ggaaaaaagg cttgcaccaa taaacgcagt ttcgggtata agcgaatgtg
     1201 tagggcgagc tttcaagcga gcccgctcca aaggccagat aatcttgatt gaggatctgg
     1261 agaatccgta ggtatcttgt gctatgaaca gacgcgttgg attgattgct ctccttgctc
     1321 tctccctcgg tctgggcata ccgccggcta cgcttgccct gagcggcgcc gacgtagtgg
     1381 cagccataga caaggctaaa gttctagatg cctcaatccg cgtcaatgcc caggtgacgc
     1441 cggaagtgat ctatatctcc acatataaaa atcccaaggc taatgacaga gactgcaaga
     1501 tcgaagccgt tttactggcc aagacggtca tggaactcga caacaagatt ccccgtgtag
     1561 aaatccgctt ttacagccag aacgccctca gccgcttcaa gaaaataagt gtttccgccg
     1621 gcgatgtgaa agcctttgcg gctggaacca tgcaggaaga ccagttgctc aactccctgg
     1681 tactggctga agaagaaacc aaagatccta acgtcagggt taccagtcac ttgcaggaaa
     1741 atcagtatgc ccggcgcaaa cgggaagtca gttcgcgcat caataaaaac gtgctggagg
     1801 tctctgccgg tctcgatccc accctggaag acgtttatct caaggcagag gctctgcgcc
     1861 tggccgagaa ggctttcgaa gccgtgggcg atgatatcga cgaggtatcc ataaccttct
     1921 acgatggctc caaggagtta tccaaccgta acgtcaccct taagcgagcg cagcagaaac
     1981 ccttttctga ggccctcaat agtgccctca aagatctgga gctgaagacc gtcaaagctg
     2041 agaaacttgc cgctgaaggg cttgatgtca ccactttcga gccttccgaa gggctctact
     2101 tgaaagagag aaaagaactg gtcggtcgct tgcgggccct taaggaagcc ggggtgggct
     2161 tcagtagaga cgtactggtc gacctcgttg ccatagaaaa tgggctcaca accctggacg
     2221 aagccgttct gagagacaag cttgccaagc tttccgcctt tatcggcaag tacgaggaga
     2281 acctcaagaa ggctaaggaa atcaaaccgg cggccgcccc taaagccagt gccgctgcca
     2341 agacgaatga tccggtctta agcgaaaacg tagatcagat gaaagcaccc gtgctggcta
     2401 atccggacgg ctacctggcc actatggccg gccgcctggc cagacgttct cccacgggca
     2461 acggcgaaga ccatcccaat
//
LOCUS       DAOQNZ010000552         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24788_length_2479_cov_5.772690, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000552 DAOQNZ010000000
VERSION     DAOQNZ010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24788_length_2479_cov_5.772690"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PKW73_10390"
     CDS             complement(<1..1171)
                     /locus_tag="PKW73_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73739.1"
                     /translation="MKNTILFSTLLAALSLAGFFNQALSREVGEKVLVDITGARKNWE
                     TATIRAKDAAGYKVEMLGVGKYVGEFYVPEAWFYDAASGATVPPGVAYGSTGSGQVKE
                     FAKVGDSVLVDVTGAKKNWETGKVIAKSGNMYTIRMLGVGDHKGEYQVPENWLFGAHK
                     EEKPVATSGSARTAPASTYASGTENQTNAKPSIWANNSSKAYSDNRTHTSSSGSSSDS
                     WKRMQADIDARIKESQKNSAGQSNTTGGGSDAWKAMQADIEARIKGSQNQAKVNAGPA
                     KPVAGSKGLSGLYLRHEQTWMGTSLNYREEHYYFMPDGRFYQGVPPEGPSAFNWAKVQ
                     AEHPELCGQYGINGDKITLSYAGSAPYSWLLKMKNSENMEMNYAPTNKVEKFGSNA"
     gene            1362..2051
                     /locus_tag="PKW73_10395"
     CDS             1362..2051
                     /locus_tag="PKW73_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA73740.1"
                     /translation="MAKILVAEDDRLAAGMLKDMLEQEQHTVELAHNGRDALDLVLSF
                     PYDLLILDLGLPEMDGTEVLDKYRGRGGKLPVLILSGRSDVNQRIHGLDLGADDYMTK
                     PYDVRELNARVRSLLRRQVVAENVSNLLRMGHIELDTARCEVLKSGQKLKLLPTEYAL
                     LEFFMRNKGRVFNASELLERIWKSDSDASENAVRTYITRLRKKIDEDGKPSLITSVYG
                     IGYKMEENGNS"
BASE COUNT          533 a          597 c          612 g          737 t
ORIGIN      
        1 gggcgttgct gccaaatttt tctactttgt ttgtgggtgc atagttcatt tccatatttt
       61 cgctgttctt catcttgagc agccagctgt agggggcgct gccggcgtac gatagagtga
      121 ttttgtcacc gtttatccca tattgtccgc acagttctgg atgttcggct tgtactttcg
      181 cccaattaaa ggcagagggt ccttccggcg ggacgccctg atagaatctt ccgtcgggca
      241 taaaataata gtgttcctcc ctgtaattca aagaggttcc catccaagtc tgctcatgtc
      301 taaggtaaag accgctcaaa cctttgctgc cagcaactgg ttttgccggt ccggcattga
      361 ccttagcctg attttgactg ccttttatcc tagcttcgat atcggcttgc atggctttcc
      421 aggcgtcact gcctccacct gtggtgttac tttggccggc gctatttttt tgactctctt
      481 ttatgcgggc atcaatatcg gcctgcatcc gcttccaaga gtcgcttgag ctgccgctcg
      541 agcttgtatg tgttctattg tctgaatagg ctttggagct gttgtttgcc caaatactgg
      601 gcttggcatt tgtctggttt tcagtgccgg aggcataggt agaggccggt gctgtacggg
      661 cagaaccgct ggtagcaaca ggtttctctt ccttatgggc gccgaagagc cagttttctg
      721 gcacttgata ttcaccctta tgatcgccta cgccaagcat tcggattgtg tacatattgc
      781 cgcttttagc aataacttta cctgtttccc aattcttttt tgcaccggta acatcgacaa
      841 gtacgctgtc tccgacttta gcgaactcct taacttgtcc gctgccggtt gagccgtaag
      901 ccaccccggg tggcactgtg gcgccgctgg cggcatcata gaaccaagct tccggaacat
      961 agaattcgcc gacatacttt ccgacaccaa gcatttcaac tttatagcct gctgcatctt
     1021 ttgcccttat ggtggctgtt tcccagtttt ttctggcgcc ggtaatgtcg accaatacct
     1081 tttctcccac ttctcggctc agcgcctggt taaagaagcc agctagagag agcgctgcta
     1141 gaagggttga aaagagaatt gtattcttca tgttgtttcc tttgtctttt gatgcccgtg
     1201 aaaccagata gaaaaagaga atccggttta gctgagaatt tggcatttta tttggagggc
     1261 gccggctgcg cattaaatct gaaagactca gggctgcttg atatcccaac tggctccttg
     1321 gttcatttac cgcgcgtgct tctatctgtt agtctttatt tatggcaaaa atattggtcg
     1381 ctgaagatga caggctagct gcaggtatgc tcaaggacat gcttgagcag gagcaacaca
     1441 cggtagagtt ggctcataat ggtcgcgatg ccctcgactt ggtccttagt tttccctatg
     1501 acctcttgat tttggatctt ggtttgcctg agatggacgg caccgaggtg ctcgataagt
     1561 atcggggacg aggcggtaag ttgcctgtgc ttatactatc tgggcgcagt gatgttaatc
     1621 agcgcattca cggcctagat cttggtgccg atgactatat gaccaagccc tatgatgtgc
     1681 gcgagttgaa tgcccgagta cgcagccttt tgcgcaggca ggtggtggct gagaacgtat
     1741 ctaatttgct tagaatgggt catatcgaat tagatacagc taggtgtgag gttctcaagt
     1801 cggggcaaaa actaaagctt ttgcccaccg aatatgcctt gctggagttc tttatgcgca
     1861 acaaaggaag agttttcaat gcttccgaac ttttggagcg gatttggaag tcggatagtg
     1921 atgcttctga aaatgctgtg cgaacttaca taactcgatt gcgcaagaag atagacgaag
     1981 atggtaagcc ttctctaata acttcggtct atggcatcgg ttacaagatg gaagaaaacg
     2041 gaaatagctg aatttggctt gatcggctga tttcacccaa ggctattttt ttcaaatata
     2101 cattgtcttt attaatatgg cgaaaagact ctcagagctt ggctagagag aaattctgca
     2161 gtttttctct catcttcttt tcatcgtatt acagcgctta tcaattcagg tttttccacc
     2221 tctctatttc ttaggctata ggggtcgttt ttcttttaaa acacccagga agctatccga
     2281 gtccaaattc tctgaactca ttcgccccga ccgccgctgt ggccgacgat ccggcacagg
     2341 gcaagtcgag cccggtcgtt acccaggccc aggtcttcga cggcgcctcc atctaccgcg
     2401 aggccttggc cgggtccagc ttcacgccgc tcttgcttag agcggacggc aataccgccg
     2461 cagtgaacgt ttcaggata
//
LOCUS       DAOQNZ010000553         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24796_length_2479_cov_3.567244, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000553 DAOQNZ010000000
VERSION     DAOQNZ010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24796_length_2479_cov_3.567244"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="PKW73_10400"
     CDS             <1..1448
                     /locus_tag="PKW73_10400"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNA73741.1"
                     /translation="PALAAGKGNKPEEKAPAMAEEVWRKNPPQLPAPRPFNMPKVETF
                     KLSNGLEVQFLEDHRFPFTSINLGFRSGSAQEPADLTGVAEMTSAMLTQGTTRLSSKE
                     LASQVEFIGGALKASTDYDYSLLSGSCLSGYSDKMLSLMSEVLLSPSFPEDELKLKKV
                     NTIQALTIKRSDPDFLIEERFSKVVFGAHPYSLVSPTEADINKLTRADLVNYHASHYV
                     PNSAVLIVVGDFDPGKMKAALEEKFGKVWQPKELPTVQEAKVPTHKGRKIYLVDRPGS
                     VQTSIKVGNLGIKRNDPDYFAMLVTNQILGGTAHARLFLNIREAKGFTYGAYSKLAAR
                     KEPGAFFAEADVRTEVTNPSLQEFFYELERMRSTKVKEEEIKAAKNYLAGSFQLGLET
                     QAGIAQRLLEAKLFDLPADYLQSYADKVMAVNVEDVQKSARKLIDAQNLVVCIVGDAK
                     KIKSELAVFAPVAVYDSDGKLSSEEAEKSLH"
     gene            1449..2291
                     /locus_tag="PKW73_10405"
     CDS             1449..2291
                     /locus_tag="PKW73_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73742.1"
                     /translation="MPNSLSVFIQGNWLLTSLLAGTVFSAALAAKPDSEPIKAAPPQK
                     VEKLTGYKLSQKDDHLGEVTVYLTDSALRIEDKRIGIVLLSKAPDWSVVAFNSRARKK
                     KTAALSKFEGFSKIGLAVSGGHYFAGVPLTKTARQSRFDKFPTSIYVSSRDFDLESAK
                     AFRLHPEDPGAIKCATMEVSTLNLKQTEKQAELLCKVFSLAKVKEIPLKYTCTDHDGS
                     KTDAMSTSKIEKAAFAASLFQMPQGYKEVANMEAVRMDSEGEGALESMLEGLDDNLGK
                     HRKH"
BASE COUNT          683 a          714 c          603 g          479 t
ORIGIN      
        1 tgccggccct ggcagcggga aaaggcaaca agcctgagga gaaagcgccg gccatggccg
       61 aggaagtctg gcgcaaaaat cccccccaat tgccggctcc cagaccattc aatatgccga
      121 aggtggaaac cttcaaactt tccaacggtt tggaagtaca gtttttagaa gaccaccgct
      181 ttcctttcac ctcgataaac ctgggcttcc gttccggctc ggcccaggaa ccggccgact
      241 tgaccggcgt agccgagatg acatcggcaa tgctcaccca gggcaccacc aggctgagca
      301 gcaaggagct ggcttcccag gtagaattca tcggcggcgc cctcaaggcc agcaccgact
      361 acgactacag cctgctgagc ggctcctgcc tctcgggcta cagcgacaaa atgctgagcc
      421 tcatgagcga agtattgctt tcccccagtt tccccgaaga cgagttgaag ctgaaaaagg
      481 tcaacaccat tcaggccctc accatcaagc gctccgaccc cgactttctc atcgaagagc
      541 gtttctcgaa agtagttttt ggtgctcatc cctactccct cgtctcaccc acggaagctg
      601 acatcaacaa gctcacacgt gccgacctgg tcaactacca cgcctctcac tacgtaccca
      661 acagtgccgt tctgattgtg gtgggcgact ttgaccccgg caaaatgaag gcggccctgg
      721 aagaaaaatt cggcaaggtc tggcagccca aagagctgcc caccgtgcaa gaagccaagg
      781 tgcccaccca caaaggtcgc aaaatctacc tggtggatag gcccggttcg gtgcaaactt
      841 caatcaaagt aggcaacctg ggcatcaagc gcaatgaccc ggactacttt gccatgcttg
      901 tcaccaacca gattctgggc ggcacggccc atgcccgact cttcctcaat ataagagagg
      961 caaaaggctt cacctacggt gcctacagca aactggccgc ccgcaaagag cccggtgcct
     1021 tctttgccga agccgatgta agaaccgagg taaccaatcc ttctctacag gaattcttct
     1081 atgaattgga gcgcatgcgc tctaccaaag taaaagaaga agaaataaag gctgccaaaa
     1141 actacctggc cggctccttc caactgggac tagaaaccca ggccggcatt gcccagagac
     1201 ttctggaggc aaaactcttc gacctgcctg ccgactacct gcaaagctat gccgacaaag
     1261 taatggcagt aaatgtggaa gatgtgcaaa agtcagcccg caaactcata gacgctcaga
     1321 atctggtagt ttgcatcgtg ggagacgcca agaaaatcaa atcggaactg gcagtttttg
     1381 cccccgttgc cgtctacgac agcgacggta agctatcatc ggaagaagcg gagaagagcc
     1441 tacactaagt gccgaactca ctttcagtgt tcattcaagg caactggctg ctaacctcac
     1501 tgctagccgg caccgtattc agtgcggcgc tggcggcaaa acctgacagc gaaccgatta
     1561 aagccgcacc accacaaaaa gtagaaaagc tgaccggcta caagctgagc cagaaggacg
     1621 accatctagg tgaggtgacg gtttacttaa ctgactcagc ccttcgcatt gaggacaaac
     1681 gcatcggcat cgtcctactc agcaaagctc cggactggtc ggtggtagcc ttcaatagca
     1741 gagcccgcaa gaaaaagaca gcagctcttt ccaaattcga aggctttagc aaaattgggc
     1801 tggccgtatc aggcggccac tacttcgccg gtgtgccact gacaaaaaca gcaagacaat
     1861 ccagattcga taaattccct acctctatct acgtgagcag ccgggacttt gatctggagt
     1921 cggctaaagc ttttcgcctg cacccggaag atcccggcgc cataaaatgt gccaccatgg
     1981 aagtaagcac attgaattta aagcagacag aaaaacaagc agagcttcta tgcaaagtat
     2041 tcagtctggc taaagtgaaa gagattcctc tcaaatatac ctgcaccgac cacgatggca
     2101 gcaaaactga cgccatgtcc acaagcaaaa ttgaaaaagc cgcatttgcc gcctcactct
     2161 ttcaaatgcc gcaaggctac aaggaagtgg ccaatatgga ggcggttcgc atggacagtg
     2221 aaggcgaagg cgccctggag agtatgctgg aaggtctcga tgataatctc ggcaagcacc
     2281 gcaagcatta aaaagcagaa aatctccggg gcgcgccccc cccttgggaa gcaagcggac
     2341 cctcatgtta atactgttat caccacagta tccttcagac ttgcaaccag tttactgcta
     2401 ttccctcacg cagtcgaagc aatcgttaaa tcctgacttg ctgcgataat atacttggat
     2461 gtaagggcgc cccatgtgg
//
LOCUS       DAOQNZ010000554         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24808_length_2479_cov_2.701320, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000554 DAOQNZ010000000
VERSION     DAOQNZ010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24808_length_2479_cov_2.701320"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="PKW73_10410"
     CDS             complement(<1..1587)
                     /locus_tag="PKW73_10410"
                     /inference="COORDINATES: protein motif:HMM:NF006133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA73743.1"
                     /translation="MKKLNGSLKGKVLFITGGSRGIGLAIALAAARDGARIVVAARTV
                     TEHEKLPGTIYTAADEIRAAGGEALAVQMDVRDEEQVKAAIAEAVRVFGGIDIVVNNA
                     GAIYLSDTGATDMKRFDLMMALNMRAVYMVTRHALPHLCRSENPHILNMSPPLNLDAG
                     WFAPQVAYTMSKYGMSLCTLGMSREFKDYGIAVNSLWPETAIDTSAVRNKLGGDKTVP
                     FSRKPEIVADAAYWLFNQDSQEVSGNFFTDVEILSKTGMTDFSAYNCVAGHKLMADFF
                     LGAAPDRMPVPVESSKATLMAIVEEARKAREKAAEAVVTEVDAGAAVETASVVVAAET
                     AAVVAAETAAVVAAEAESVSEPAANPYKSFVVSRTGDVAYLSFNNPARLNCLGVNFFE
                     ELPRAITAMEESGVRALVIEAAVGRHFSSGLDLNVLANESLLKVDTEGDKIRLNGLIL
                     SWQKALSSVANARFPVIAAVDGLCLGAALDLVSACDFCLATKDAQFSIEEINVGLMAD
                     LGSLQRLPRRIPESVVRYLAF"
     gene            complement(1690..>2479)
                     /locus_tag="PKW73_10415"
     CDS             complement(1690..>2479)
                     /locus_tag="PKW73_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73744.1"
                     /translation="ASSRVLFRVELSAEDFSLESPSMSPESLEGVAAEICRTATALGL
                     KLEICPCDNANFLRGEWAPDEKYLGEADAVQDMDGYEQWWELEVSKCGYQGRCLPKVP
                     CISTPTYTTGAEDVLKVLQTAYRIAGIVPSQASIVEALRKDFPQMRAQSHLADVRECL
                     GFAAAGVDTLFQVRLEASDFKKERAVPDRRKLIGSARELVNMASFLGLVSTVRRVDNG
                     LKVRSPDQGLFASAEKLPGYEEWLVLEISLSAPEGNGLPYHQSA"
BASE COUNT          560 a          736 c          648 g          535 t
ORIGIN      
        1 gaaggccaga tagcgcacca ccgattcggg aatgcgacgg ggtaaacgct gcaagctacc
       61 cagatcggcc atcaagccga cattgatttc ctcgatggaa aactgcgcat ccttggtggc
      121 caggcagaaa tcgcaagcgc tgaccagatc aagagcagcc cccagacaga gtccatccac
      181 ggcggcgata accggaaagc gggcattggc taccgaactc aaggctttct gccaggagag
      241 aatcaagccg ttcagacgaa tcttgtcgcc ctccgtgtct accttgagca ggctttcatt
      301 ggcaagcacg ttcaaatcga gcccgctaga aaaatgccgg cccacagctg cctcaatcac
      361 gagtgcacgc acccctgact cttccatggc agttatagct ctgggcaatt cctcgaagaa
      421 attcacgccc aggcaattca accgcgccgg attattgaag gacaggtagg ctacatcacc
      481 agtgcgactg acaacaaagc tcttgtaggg gttggcagcg ggctcagaaa cgctttctgc
      541 ttccgccgcc acaacagccg ccgtctccgc cgccacaaca gccgccgtct ccgccgccac
      601 aacgaccgag gcggtctcca cagcggcacc ggcatcaact tccgtgacaa cggcttcggc
      661 ggccttctcc ctggcttttc tggcttcttc cacgatagcc atgagggtag ccttagagct
      721 ttccaccggc acaggcattc tgtcaggagc agcgcctagg aagaaatccg ccataagctt
      781 atggcctgcc acgcagttat aggcggaaaa atccgtcatg cctgttttag agaggatctc
      841 cacatcggtg aagaagttgc cgctcacttc ctgagaatcc tgattgaaga gccagtaggc
      901 tgcatcagcc acgatttccg gtttgcgaga aaacggcaca gtcttatcgc cacccagttt
      961 attgcgcacg gcactggtgt cgatggctgt ttcaggccag agcgaattga cggctatacc
     1021 gtagtcttta aactccctgg acatgcccag ggtgcagagg ctcatgccgt acttggacat
     1081 agtgtaagcc acctggggag caaaccagcc cgcatccagg ttgagaggcg gcgacatatt
     1141 gaggatgtgc ggattctcgc tgcggcaaag gtgcggcagg gcatgcctgg tcaccatgta
     1201 aacggcccgc atgttgagcg ccatcatcaa atcgaagcgc ttcatatcgg tggcgccggt
     1261 atcgctcaga tagatggcgc cggcattatt gacgacgata tcgataccgc cgaacacgcg
     1321 cacggcttct gcaatcgctg ccttcacctg ctcttcatcg cgcacatcca tttgcacggc
     1381 gagagcctca ccaccggcag ctctgatttc gtcggcggcg gtatatatgg tgcctggcag
     1441 tttttcgtgt tccgtcacag tgcgggcggc aacaacaatt ctagcgccat cccgggcagc
     1501 ggccagggca atagccaggc cgataccgcg agagccgccg gtgatgaaca gtacttttcc
     1561 tttgagactt ccatttaact ttttcatgtt ttcctccacc acatctagtg gcttgttttt
     1621 cctagtcagt cggcttgaga acccaagccg actgttaatc taaggtttag ttagtcagcc
     1681 ggacttttct caagccgact ggtgataagg caggccatta ccctcgggag cactgaggct
     1741 gatttccagc accagccatt cttcatagcc gggcagtttc tcagcactgg caaacaggcc
     1801 ctggtcgggt gaccgcactt tcaggccgtt atccacgcgc ctgacggttg agacaagacc
     1861 caggaaggaa gccatattca ctagctcacg agcgcttccg atcagttttc tacgatcggg
     1921 cactgctctt tccttcttga agtcggatgc ttccaggcgg acctgaaaca aagtgtccac
     1981 acctgcagca gcaaagccca ggcattcgcg gacgtcggct aaatgagact gggctcgcat
     2041 ttgcggaaag tccttgcgta aagcttccac aatggaagcc tggcttggaa cgatgccggc
     2101 aatacgataa gcagtctgca acaccttgag cacatcttca gcaccggttg tgtaggtagg
     2161 tgtgctgata caaggtacct ttggcaggca tcttccttga tagccgcatt tgctgacttc
     2221 cagctcccac cactgctcat aaccgtccat gtcttggacg gcatcggctt cacctaagta
     2281 cttctcgtcc ggtgcccact ctccccgcaa gaaattggca ttgtcgcaag ggcagatttc
     2341 cagcttcaag ccaagggcgg ttgccgtccg gcaaatttcg gcggcgacac cctccaggct
     2401 ttccgggctc atgctcggac tttccaggga gaagtcttca gccgaaagct ctacccgaaa
     2461 cagaactcgg gatgaagct
//
LOCUS       DAOQNZ010000555         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24868_length_2476_cov_2.994217, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000555 DAOQNZ010000000
VERSION     DAOQNZ010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24868_length_2476_cov_2.994217"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKW73_10420"
     CDS             complement(<1..323)
                     /locus_tag="PKW73_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA73745.1"
                     /translation="MKTLIKNGHIITAVDDYHGDILIEGERINMIGECLDMEVDQIID
                     AEGLLVIPGGIDAHTHMDMPFGGTTSADDFESGTRAAAHGGTTTIIDFAIQNKGQSLY
                     EALDT"
     gene            646..>2476
                     /gene="dnaG"
                     /locus_tag="PKW73_10425"
     CDS             646..>2476
                     /gene="dnaG"
                     /locus_tag="PKW73_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNA73746.1"
                     /translation="MTSEVIAEVRMRAGILEVISETVVLKRTGKEYKGLCPFHGEKSP
                     SFHVNPDKGIFKCFGCGEGGDVFAFVQKTKGLDFIDSVRELARKYGVKLAESVEDRKE
                     YDRRSQILMLYQQASEYYIRLLNDPSEGAFARKYLQDRGLSQETIDKFKLGVAPNSWD
                     GLLRYLTEKTKVAPSTLEEAGLIRRRQESSGHYDLFRNRLMIPICDSEGRVIAFGGRT
                     LGDDQVKYLNSPETPIYHKGLHLFGFNLAKESVKEKDSVIVVEGYFDAITSHQFGFTN
                     TVATLGTALTEQQAKLLVRYTDSKRVYLSFDADAAGVRAVERGIEMLSQIAEGIGIEL
                     RVIAIPGGKDPDECLRSGAAGVEAFNRAVTDAALMIDYQLEQAIKGIDVDLRTGRIEA
                     ARRVVPILALIKNAVGRGEYIRLWAMRLRVREEEILSDVSQYRRANRLDGARPAAGGG
                     WRSGQGYNSNYNNQGYNRSGSGGQSWGKGYGGRGGASGQGGYSGQGGSQGGYAGQSSG
                     SQGGYGGPSSSMAQGGRSTPGARPADKSADFDESFAESFADPFAAAHASEDESGLGSD
                     FRGEEFEKEVAPPVRPQSGREPGKVNAPPQNNQNQPAYGSQGYG"
BASE COUNT          538 a          608 c          767 g          563 t
ORIGIN      
        1 caggtgtcga gagcttcata aagcgattga cccttgttct ggatggcaaa gtcgataatg
       61 gtggtggtgc cgccgtgggc ggcggcgcgg gtacccgatt cgaagtcgtc ggcggaggtg
      121 gtgcctccga aaggcatatc catatgggtg tgggcgtcta tgccgccggg aatgactaat
      181 agaccctcgg catcgatgat ctggtccacc tccatgtcga ggcattctcc gatcatattg
      241 atgcgttcgc cctcgatcag aatatcgcca tgataatcat ccactgcggt gatgatgtgg
      301 ccattcttga tcagtgtttt catgcctacc tccggtgtag ccgaattgct ggtgcttgat
      361 accgcttgtg agcaggagct ctaatggcgc cgcgcattgg ctcaaaatta tatacggtta
      421 agggcttccc gtgtatttgc ggttctcaag ggtcgaggcg gcctgctgcg gttgcattca
      481 aattcggcaa tcagcccggg ttttttgcag gggcgcaggt gctatttcgt gctataaact
      541 gagaattaaa atgtaagggg gaagactttt gccgcgccct tgcagaatac atacacaaag
      601 tgcaaagagc gcgatatttc ccaaggggga cctggcttga agggcgtgac atctgaggtc
      661 attgctgaag tgcggatgcg ggcaggcatc ctggaagtaa tttcagaaac agtcgttttg
      721 aagcgcaccg gcaaggaata caagggactt tgcccctttc atggcgagaa gtcgccttct
      781 tttcacgtca atcccgataa gggcatcttc aagtgctttg gctgcggtga agggggcgat
      841 gtttttgcat ttgttcagaa aaccaagggg ctcgatttta tcgactcagt gcgtgaactg
      901 gctcgcaaat acggcgttaa gttggccgag tctgtggagg atcgcaagga gtatgaccgg
      961 cgttcgcaga ttctcatgct ctaccagcag gcaagcgagt actacatccg tctgttgaat
     1021 gatcctagcg agggtgcttt tgcgcgcaag tacttgcagg accgcgggct ttcgcaggag
     1081 actatcgaca agtttaaact gggtgtggcc cccaatagtt gggacgggct cttgcgatac
     1141 ttgaccgaga aaaccaaggt ggcacccagc actctggaag aagccggctt gattcggcgt
     1201 cggcaggaat caagcgggca ctacgatctc tttcgcaacc gtttgatgat tcccatttgc
     1261 gacagcgagg ggcgtgtcat cgcatttggt ggcagaaccc ttggtgatga tcaggtcaaa
     1321 tatctcaact cgccggagac tcccatctat cacaaaggtc tccacctctt tggttttaac
     1381 ctggccaagg agtcggttaa ggagaaggat tcggtaatcg ttgttgaagg atatttcgac
     1441 gccattacct ctcaccagtt tggttttacc aacactgtgg ctactctggg aacggctctg
     1501 acagagcagc aagccaagct tctggtcagg tacacggaca gcaagcgggt ctacctgtct
     1561 ttcgacgccg atgctgccgg ggtgcgtgct gttgagcgcg gtattgagat gctcagccag
     1621 atcgccgagg gcattgggat tgaactgaga gtgatagcca ttcccggcgg caaagacccg
     1681 gacgaatgct tgcgctcagg tgctgccgga gtagaagcct tcaatcgggc tgttaccgat
     1741 gcggctttga tgatcgacta ccagctcgag caagccatca agggtatcga tgtagatctg
     1801 cgtaccggta gaattgaagc ggccaggcga gttgttccca tcctggccct tatcaaaaac
     1861 gccgtcggtc ggggtgagta catccgactc tgggctatga gattgagagt gcgagaggaa
     1921 gaaattctct ccgacgtgag ccagtatcgg cgcgccaatc gcctggatgg tgcaagacct
     1981 gccgccggcg gcggatggcg gagcggtcag ggctacaatt ccaattacaa caatcagggc
     2041 tacaatcgct ccggttccgg tgggcagagc tggggcaagg gttatggcgg tcggggcggt
     2101 gcttccggcc agggtgggta ttcgggtcag ggcggttccc aggggggata cgcgggccag
     2161 agcagcggct ctcaaggtgg atacggcgga ccaagctctt ccatggctca aggggggcgc
     2221 tcgactccag gagcacgacc ggctgataaa agcgcggatt ttgacgaatc tttcgcagag
     2281 tcttttgcag atccttttgc cgcggcacac gcttctgaag atgaatcggg tctggggtca
     2341 gactttcggg gcgaggaatt tgagaaggaa gtggcgccgc cggtccgtcc gcagtcgggc
     2401 cgggaaccag gaaaggtaaa cgctccgccg cagaacaatc agaaccaacc ggcctacggc
     2461 agccagggct atggcg
//
LOCUS       DAOQNZ010000556         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_24886_length_2475_cov_3.870661, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000556 DAOQNZ010000000
VERSION     DAOQNZ010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24886_length_2475_cov_3.870661"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..791
                     /locus_tag="PKW73_10430"
     CDS             45..791
                     /locus_tag="PKW73_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73747.1"
                     /translation="MQTIKRAVGIFLVALSAAACGLAVFTSARQQSVSTDKDRLEKKL
                     EKTFFFSESFRPDFTTSVDIAEAKLIDELFEDLQDGRSERVVEKAKTCLQEGNRSWNF
                     YFALGALLIDCKSYTLAQEAFSVSDRKLTETIPDAYVAENYLKQDINLDPRAALTEAY
                     LHELQELEKDKKAPPQARAALYAKALKYSHPHGYVYKMYSDFLREQGKKDEAEQALKR
                     WDLSPAFKERRNLDLSALKNPCALVQTHQQ"
     gene            complement(782..2458)
                     /locus_tag="PKW73_10435"
     CDS             complement(782..2458)
                     /locus_tag="PKW73_10435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNA73748.1"
                     /translation="MSALGRKQKSQQASERQALLLLSAMVVSAAVAACFEPFKQYVEE
                     AGFYQPPDWFAIVCGICAGILSVVWNEVVNRSLDKRGLNETPAPIHYVAFLLVISFVT
                     PIAEEIIFRGVLQQLIGYSPASILFALAHFESASVWNMLLSKFPLGLFCGGAFVVSHN
                     LWSAVIAHTINNVWFTVPEYFRDRDVVKGVKLSLDGNYEGAIEILSRTKGLEGNRYAL
                     RNRASCFNQIKKCNEAIADATKAIELGDNYAYLMRCSSYLLLGKTELAEQDVVFAKKK
                     YPKCSEPYLLAGVVYLQKLEFTAARSEFLMSIEIAETPVGWALCGCAALSLFQYDLCL
                     EDCRKALSLNPSYYFAFQVKALALLALNKLEDAMICSNQAVENASPHGLPETLAGRAM
                     IFIRVGDKESALADLSNAIAAVPESKMPNLSRAFIYIQEERFEGAFAELDQLSRLVET
                     PLDRSYHLGYRALAKVLKGDVESGLADAIEANSIYPSRPDLLNILGLALIRNGQAGQA
                     VEELNRAIELDPYNAESYWFRHLAYESLGQDELASADKGIAEKYCYKPYC"
BASE COUNT          719 a          645 c          566 g          545 t
ORIGIN      
        1 tcaatacggc ccatgggcaa gattgaaata cttgagtggg atttatgcaa accatcaaaa
       61 gagctgtggg cattttctta gtggcgctga gcgcggcggc ttgtggtctg gcagtattca
      121 ccagcgcaag gcaacaatct gtatcaacag acaaagatcg gctagagaag aaactagaga
      181 aaaccttctt tttctccgaa tccttccgcc cagacttcac aacttcggtg gacattgcag
      241 aggcaaagct tattgatgaa ctctttgaag atctgcagga tggaagaagt gaaagagtag
      301 tggaaaaagc aaaaacttgc ctgcaggaag gcaaccgcag ttggaacttt tatttcgcgc
      361 tgggtgccct gctcattgat tgcaagagct acactctcgc ccaagaagcg ttttccgtat
      421 cggaccgcaa gctgactgag acaattcctg acgcttatgt cgcagaaaac tatctcaaac
      481 aagacatcaa cctcgacccc cgtgcggctc tgaccgaagc ctatctgcac gagttacaag
      541 aactagaaaa ggacaagaag gcgccgccac aggcaagggc cgccctctac gcaaaagcgc
      601 ttaaatactc ccacccccat ggttatgtct acaaaatgta ctcagacttt ctacgtgagc
      661 agggaaagaa agacgaagca gagcaagcgc tcaagcgctg ggacttgagc ccggctttca
      721 aagaacgacg caatctggat ttaagtgcgc tcaagaatcc ctgcgctctg gtgcagacgc
      781 atcaacagta aggtttgtag caatatttct cggcgatacc tttgtcggca gaggctaact
      841 catcttgccc cagactttca taggccaggt gccggaacca atacgattca gcgttatatg
      901 gatcaagctc aatagccctg ttcagctcct caacggcttg cccggcctga ccgtttctaa
      961 tcagcgccag tcctagaata ttcaagagat ccgggcgaga tggataaata ctattcgcct
     1021 caatggcatc ggccagtccg ctttctacat cgcccttcag aactttggca agagcacggt
     1081 atcctaaatg ataggagcgg tccaacggag tttcgacaag ccggctcagc tgatcaagct
     1141 cggcgaaagc cccctcgaaa cgctcttcct gaatgtagat aaaggctctc gataaattcg
     1201 gcatcttaga ctccggcacc gcagcaatag cgttggatag atccgcaagc gcgctctcct
     1261 tgtctccgac gcgaatgaaa atcatagctc taccggcgag ggtctctgga agcccatgag
     1321 gggaagcgtt ttcaacagcc tggttgctac aaatcatggc atcctccagc ttgttcagag
     1381 caagcaacgc aagcgccttc acctgaaagg caaaataata actaggattc agcgacagag
     1441 ctttccggca atcttcaaga cacaaatcat attggaacaa actaagagcg gcacaaccac
     1501 aaagagccca acccacagga gtttcggcaa tctcaataga catcagaaac tcacttctgg
     1561 cagcggtaaa ctccagtttt tgcagataga ccacacccgc caacagatag ggctcactgc
     1621 actttggata ctttttcttg gcaaagacca catcctgctc agccaattca gtcttaccaa
     1681 gaaggagata gcttgagcag cgcatgagat atgcatagtt gtcgccaagt tcaatagcct
     1741 tagtagcgtc cgctattgct tcattgcatt tcttgatctg attgaagcaa ctggctcgat
     1801 tacgcagagc atagcgattt ccttcaaggc ccttggtcct agacaaaatc tcaatagcgc
     1861 cttcataatt gccatctagc gaaagtttca ccccttttac cacatctcga tcgcgaaagt
     1921 actccgggac ggtaaaccag acattattga tggtatgtgc aataacagcg ctccaaagat
     1981 tgtgcgaaac aacaaaagct ccaccgcaaa aaaggcccag cgggaacttg gacagaagca
     2041 tattccagac gcttgcagat tcgaaatggg ccaaagcaaa taaaatagaa gccggagaat
     2101 aaccgatcaa ctgctgtaaa acaccgcgaa aaataatttc ttcggcaatc ggagtgacaa
     2161 aactaattac caggaggaag gcgacataat ggataggtgc cggcgtttca ttgagcccgc
     2221 gcttgtctag ggaacggttt acgacttcat tccacacgac actcagtatg ccggcacaga
     2281 ttccgcacac aatagcgaac cagtcaggag gctgataaaa tcccgcctcc tccacatact
     2341 gcttgaatgg ttcaaaacaa gctgcaactg cggcactgac caccatggct gacagaagaa
     2401 gcagagcttg cctttctgaa gcctgttggg atttttgctt tctacccaaa gctgacatta
     2461 ttcacggtcg tcaca
//
LOCUS       DAOQNZ010000557         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25092_length_2465_cov_3.264730, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000557 DAOQNZ010000000
VERSION     DAOQNZ010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25092_length_2465_cov_3.264730"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PKW73_10440"
     CDS             complement(<1..1053)
                     /locus_tag="PKW73_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNA73749.1"
                     /translation="MNWGDIAIAVSGFTIIVMVLSDVFQSIIVPHYRPKGTRLSPLLI
                     SGILWQPLRQFIKAREMKQKAEADLSLFAPAAIMCLLACWLTLMTTGFALLLYAERAN
                     IKPQLQSIEEALYFAATSVLTIGFGDVVACSALSRLTVIAAAMAGLVLLAISVSFMFA
                     IQSHFHNREVSSQIISSRHGHSKNGAVLYLRLLDDNSAAGCLELCERWVTDIYQSHSA
                     YPILLYFRSRSSRASWLVQLCVALDAAAIAIAMNNSRHMALFNSIYDNGSRAVDVFAT
                     YLTLHPRIKNVTQEPALFQSLFKNLGASDIEQTAAYFSVLRQRYFPALSSLSDFFLIE
                     LPPLVSADAQILLQARS"
     gene            1356..1583
                     /locus_tag="PKW73_10445"
     CDS             1356..1583
                     /locus_tag="PKW73_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73750.1"
                     /translation="MKDSYMVFVLLAVMLTPFLPLMLKGKCPTCGKRKLETLETPQAT
                     DENAICYTSHHRCKACSAMFTRERSGPLTPV"
     gene            complement(1672..>2465)
                     /locus_tag="PKW73_10450"
     CDS             complement(1672..>2465)
                     /locus_tag="PKW73_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73751.1"
                     /translation="GETMADDLSRLKLVVPALIGFSLSDFNISELKVFKDDDIAGIKI
                     EDLDSAEQLEQIVELIKPWKRLAFLLFHKGTLKETNLSLLDQLHHLDQLELVECETNK
                     NVLANLNCLTKLRIFTMVNGTEPTPERDAPQIARILERLAQNKRLSILNLKGQPISHK
                     TMRRVLMAPSMKTLIVFGYRWNEDNLKTLKTESNIQFLAVKEADIPEKLIPLLAGAKH
                     LNGLAIEDVNSRQTAARLLALRPLMVNARGRKIDMEVTLKPGIDY"
BASE COUNT          602 a          562 c          652 g          649 t
ORIGIN      
        1 gcttctggcc tgcaaaagaa tctgggcatc cgctgacacc agaggtggta gctcgatcaa
       61 gaaaaagtcc gaaagggaag agagggctgg aaagtatctc tggcgcaaga ccgagaagta
      121 ggcggccgtt tgttctatat cggaggctcc caggttcttg aagagcgact gaaagagggc
      181 gggttcctgg gtcacatttt tgatgcgagg atgtaaggtc aggtaagtgg caaagacatc
      241 cacggctcga ctgccgttgt cgtagatgga attgaagaga gccatgtgtc tggagttatt
      301 catggcaata gctatggctg ctgcatcgag ggccacgcaa agttgaacca gccaggatgc
      361 ccgtgaactg cgggagcgga aatagagcag aatcggatag gccgagtggc tttgatagat
      421 gtcggttacc catctttcgc aaagttccag gcagccggcg gcgctattgt cgtcgagcag
      481 ccgcaggtac aagacggcac catttttgct gtgcccgtgc cgggaggaga ttatctgaga
      541 agaaacctcg cggttatgaa aatgggactg aatggcaaac atgaaggaca ctgaaatagc
      601 cagcaagacc agtccggcca tggcggcagc tataacggtc agtctggaca gggcagagca
      661 ggctacaaca tctccgaagc cgatggtgag caccgaggtg gcggcaaagt atagagcctc
      721 ttcaatgctc tgcaattgcg gcttgatatt ggcgcgctcg gcatagagca gcagggcaaa
      781 acctgtggtc atcagggtca gccagcatgc gagcaggcac atgatggccg ccggtgcaaa
      841 caagcttaag tctgcctcag ctttttgctt catctctctg gcttttatga attgccgcag
      901 gggttgccag agtatgccgg aaatcaagag gggcgaaagg cgtgtgccct tgggtcggta
      961 atggggcacg ataattgatt ggaagacgtc tgagagcacc atcactatga tggtgaagcc
     1021 ggaaactgca attgctatgt cgccccaatt cataatttag tgacttgctc ctgaaggtgt
     1081 tctttcaaaa ctgccgggca ttccggccaa atctctggag aggtggtcaa ctttccgagt
     1141 gtcaaacagg agcttaattc cttttcccag atgttaccaa gagatgtcct ccctttcatt
     1201 aatgaaagca ccgacagaac tgacaaaata gaccgacctt cgtcttacat ctctctcaga
     1261 aaaggctttc gaacttgata ttccgccttt tcaaggtgag aatgcgacaa aattatgtgg
     1321 attgtattgg ttggcttttc gagatggctt gatctatgaa agattcttat atggtttttg
     1381 tgctcctggc agtgatgctg acgccattcc tgccattgat gctcaaaggt aagtgcccga
     1441 cctgcggcaa gcgtaagctt gagacccttg aaacaccgca ggctaccgat gagaacgcca
     1501 tatgctatac cagtcaccac cgttgcaagg cttgctccgc gatgttcacc cgcgaacgca
     1561 gtggtccgct gacgccggtt tagattactt gtagctagat cgcttttagc tagattcctt
     1621 gtaactggat tccttatgta gctagtttgc ttgttgctaa tttcctgatt attaatagtc
     1681 aattcctggc ttcagggtga cttccatgtc gatttttctt cctctggcat taaccataag
     1741 tggtctcagg gccaacagac gcgccgccgt ctgacgggag ttcacatctt cgattgcaag
     1801 accgttgagg tgtttcgcac cagctagaag aggaattagt ttctctggaa tgtctgcctc
     1861 ctttactgcc agaaattgaa tattgctctc ggttttaagg gttttaagat tgtcttcgtt
     1921 ccacctgtag ccgaagacta tcagtgtttt cattgagggt gccataagca cgcgccgcat
     1981 tgtcttatga gaaattggct gtcccttaag gtttagaatg gaaagtcgtt tgttctgcgc
     2041 aaggcgttca agtattcttg caatctgagg agcatctctc tccggagtgg gctcagttcc
     2101 atttaccatc gtaaaaattc taagctttgt caggcaattc agatttgcca gtacattttt
     2161 attcgtttcg cactcaacca gttccagctg atccaaatgg tgcagttggt ccagtaagga
     2221 aagatttgtc tccttgagag tgcctttgtg aaagagtagg aatgcaagcc gtttccaggg
     2281 tttgataagc tctacaatct gttcgagttg ctctgctgaa tccaaatctt caatcttgat
     2341 gcctgcgata tcgtcgtcct taaagacctt gagttcggag atattgaagt cggaaagtga
     2401 aaagcctatc agtgccggca ccacaagttt cagtcggctc aagtcatcag ccatcgtttc
     2461 gcctg
//
LOCUS       DAOQNZ010000558         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25226_length_2459_cov_3.059484, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000558 DAOQNZ010000000
VERSION     DAOQNZ010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25226_length_2459_cov_3.059484"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..870)
                     /locus_tag="PKW73_10455"
     CDS             complement(49..870)
                     /locus_tag="PKW73_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73752.1"
                     /translation="MNAMNETAMYWQTLRNNRDAVMALQACQDEIFEVAALFTRTQGD
                     SIVKLTPKGQSLHVRKARGALKAMRTFIESQMDNLDEAGKFHFSERKMAAVALYRAYI
                     KACLALAYVDYWEKSHHFRNFAVLAQKVEYYADAVMGSNWEFFAPPIFFNPSEALLPG
                     SEQNLQLLIGYAAAAHGYAMALHGQLNFMPPRTVFTPVELTDEERHLEETVFDCIVRS
                     DIATGCGQERAVDLHFAYTQRNSKSAHSRPAHPDIRRLVGFQKTRGNRKRAKQKR"
     gene            complement(1035..1616)
                     /locus_tag="PKW73_10460"
     CDS             complement(1035..1616)
                     /locus_tag="PKW73_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNA73753.1"
                     /translation="MTPASTIYKNLLLLPADLNRLKVEFDIESAPDQWALMLNDKGRL
                     PSEESLVFYGQTTAYSEAIKLVQDVQDGRLISEHFELNLEAIPHTVHSIVLVAASEME
                     PGTGPKRMNLTKEACIRLTGLDNTRWDVTFKPSKVLKGKAKRLTWGAFVRQEGQWRFE
                     PYFRVERQFGISTILKAHGARIDKTEEGRSLSV"
     gene            complement(2052..>2459)
                     /locus_tag="PKW73_10465"
     CDS             complement(2052..>2459)
                     /locus_tag="PKW73_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73754.1"
                     /translation="VPHRLKETRHIAAPHRRSHRNRLSLKDLVKPGIHPSIQLLEFQI
                     KLLRQSCKCLIDSGKKTSLSKVIVGIEVQGVSARRWLYGKEFGSHSNTPHVVEKPEKV
                     QTVVVACATQTRSRQQYKSLNRLLWMNGKLTNS"
BASE COUNT          490 a          584 c          676 g          709 t
ORIGIN      
        1 agcgatccag cgcaagcgtc cttgcctgcg cctttggaag tgcgttattt agcgcttttg
       61 ttttgcgcgt ttacggttgc ctctggtttt ctggaagccg accaggcgcc ttatatcggg
      121 atgtgccggg cggctgtggg cggacttgct gttccgctgg gtgtaggcga agtgtaagtc
      181 cacagcccgc tcctgtccgc aaccagtggc tatgtcgctt ctcacgatgc agtcgaagac
      241 ggtttcttcc agatgtcgtt cttcgtctgt taattccaca ggagtaaata cagtgcgggg
      301 cggcatgaaa ttgagctgtc catgcagggc cattgcatag ccatgggcag cggctgcgta
      361 tccgatcaaa agttgcaggt tctgttcgct tccgggtagg agtgcttcac tcggattgaa
      421 aaatatcggt ggagcgaaaa attcccagtt acttcccatt acggcatcag cgtagtactc
      481 gaccttttga gccagtacgg cgaaattgcg aaaatggtga gacttttccc agtagtctac
      541 gtaagccagg gccaggcagg cttttatgta agcacggtaa agcgccactg cggccatctt
      601 gcgctcactg aagtgaaatt tgcctgcttc atcgagatta tccatctggc tttcgataaa
      661 agtacgcatg gctttgaggg ccccgcgcgc cttgcgcaca tggagagact gacctttggg
      721 agtgagtttg acgatgctgt ctccttgggt tctggtgaag agagcggcca cttcgaatat
      781 ctcgtcctgg caggcttgga gtgccataac tgcgtctctg ttgtttcgta gggtctgcca
      841 gtacatggcg gtttcgttca ttgcgttcat gtcttcgctc gttcaaaaga aaggtccccg
      901 gacggatcct ttcggttaat ggcgacttgt ccggagtcgc gcagctgcaa ctgtaaagtt
      961 gcgtcatgct ggactgctgt cgcaaaagag tttttgttct acaacgcagt cctctttgcc
     1021 tcagggcagg ggtgtcaaac gctgagggaa cgaccttctt ccgtcttatc gattcttgct
     1081 ccatgggctt tgagaatggt gctgatgccg aactggcgct ccactctgaa gtagggctcg
     1141 aaacgccatt gtccttcttg tctgacaaag gcgccccagg tgaggcgttt ggctttgcct
     1201 ttgagaacct ttgagggttt gaaagtgacg tcccatctgg tgttatccag accggtcagt
     1261 cttatgcacg cctctttggt gagattcatg cgcttggggc cggtgccggg ttccatttcg
     1321 ctggctgcca cgagcacaat gctgtgcacc gtatgcggta tggcttccag gttcaattcg
     1381 aagtgttcgg aaatcagccg gccgtcctgc acatcctgca caagcttgat ggcttcgctg
     1441 taagcagtag tttgaccata aaataccagg ctctcttcag agggcaggcg tcctttatcg
     1501 ttcagcatga gagcccattg gtccggcgcg gattcgatat cgaactcgac cttgaggcga
     1561 ttcaggtcgg ctgggagaag cagcaaattt ttatatatag ttgaagctgg ggtcatgatg
     1621 gtctccagtg aggctaatga aaaaattgag ttgtggtggg tgttctaatt tgcattccgg
     1681 ttgactgtgc tcatcctttg ccgtttgaat gcggcttgaa agggcttcag gtggttgtgt
     1741 ggtttgtaag ggagagagtt ttaccggttg agcagttttt agagaacaat tcatcctgct
     1801 cccggttgca gtcttgatac aagagggatt aaggcaaaac ccaatactct tcaccttttt
     1861 gaggtagtca agcggaaagt gtctcgtgct attctcgcaa accgaccgcc ttgatttggg
     1921 ccgttttatt gcatggggtc tgggtcttcc gcacctggaa gaagctgaag tgcgggcagc
     1981 gtgttaacta accgccctgc ttgcttgtaa ctgccggctc tttataagca gaatgagttt
     2041 ttcctgttgt ttcaactgtt cgtcaatttt ccattcatcc agagaagcct gttcaagctt
     2101 ttgtattgct gcctcgacct tgtctgtgtc gcgcaagcaa ctacgactgt ctggaccttc
     2161 tctggttttt caacaacatg aggagtatta ctatgacttc caaattcttt cccatagagc
     2221 catcttcggg cactgacccc ttgaacttcg atccccacaa ttaccttgct gagagaggtt
     2281 tttttgccgc tgtcgatcag gcatttgcag ctttgacgga ggagcttgat ctggaattcg
     2341 agcaattgaa tgctcggatg aatgccaggt ttgacgagat ctttcaatga aagcctgttt
     2401 ctatggcttc ttcggtgtgg tgctgctata tgccgggttt cttttagccg gtgcggcac
//
LOCUS       DAOQNZ010000559         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25346_length_2453_cov_3.399500, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000559 DAOQNZ010000000
VERSION     DAOQNZ010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25346_length_2453_cov_3.399500"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2453
                     /locus_tag="PKW73_10470"
     CDS             <1..>2453
                     /locus_tag="PKW73_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNA73755.1"
                     /translation="AFLILGSKSGVHYYMGISLPSAVESNEPGNDPTSICYHSLKSIL
                     HSVYGGVDVFKKPFSAEDIHAMIEPMTKYTGVVTGIPALKSTGGDTTESEQIERLAAG
                     LSGHDFGMLILAVPIPSKNVTKEETAVVDQIQRAQENEDPEKKRKIKYYLELQDSYLK
                     HIQLGTALGMWLTVPYFFSPKRDVFVRLQSLLRATYVDETSRPTPLRTHEVRGLSEHI
                     QRFGLLRSKRDGEYFQELLEYRFLSPLNSRMLSAFVHLPKREMPGFRIRRSAEFSLAE
                     IPPKDPNRVIAIGNILDRGEDTGNLYYIDVDALQKHTIVCGVTGGGKTNTCFYLVGQL
                     WRYGIPFMVCEPAKSEYRHMMLMSETFKGVGQAFSLGDETVSPFRLNPFEIMKGVKVQ
                     THLDALKSVFNASFEMYSPMPQVLEKALNSIYAVRGWDLINNVNRRLPPGMSVGDPDC
                     PNEIYPTMKDLYEIIDPIVESFGYSDRIGPDVQAALKARIGSLLIGGKGQMLNTRRSI
                     PMGELFGKPTVVELKMVSEDSEKSFLMGMLLVFLYEYREALGPHDHLQHVMLVEEAHR
                     LLKNVPTGQSGESANPAGKAVEFFTNMLAEIRSYGQGFIIADQIPNKLAPEALKNTNL
                     KIMHRIVAVDDRDSMGGAMNLDDVQKRHVTALGQGRALVYAEKMEQPYHLQIMFDKTK
                     EVPPPETPEESDEVVRQAMKGLNIIGSYDRHLGCKFCLHRCDSQILDTAIPVADDPIF
                     RQVYNRYVLSTLKDLTQLVHFRAQIIHEIQRVIGGRARTGNITGITWCALTQATERYF
                     ERKGENNYWFYDQVREQHL"
BASE COUNT          614 a          692 c          604 g          543 t
ORIGIN      
        1 ggccttcctc attttgggca gcaaatcggg agtccattac tacatgggca tctcccttcc
       61 cagcgctgta gagtccaatg agccgggcaa tgatcctact tctatctgtt atcacagcct
      121 caagtcgatc ctgcacagcg tctatggtgg agtagatgtc tttaaaaagc ccttttcggc
      181 tgaagacatt cacgccatga tcgaaccaat gaccaagtac accggcgtgg taacaggcat
      241 tccggccctg aagtccaccg gcggtgatac caccgaatca gagcaaatcg aaagactggc
      301 agccggtctg tctggtcatg acttcggcat gttaatcctg gccgttccaa ttccaagcaa
      361 aaatgtcacc aaggaagaaa cggctgtagt ggaccagatc cagcgggccc aggagaacga
      421 agaccccgag aagaaaagaa aaattaagta ctatttggaa ttgcaagatt cctatctcaa
      481 gcatattcaa ctcggcaccg ctctgggaat gtggctgact gtgccttatt tcttctcacc
      541 caaacgggac gtatttgtca ggcttcagtc actcctgaga gccacctatg tagatgaaac
      601 cagccgtcct acccctttgc gcacccacga agtgcggggg ctgagcgagc acattcaacg
      661 tttcggactg ctgcgctcca aaagagacgg cgaatatttc caggagttgc tcgaatatcg
      721 cttcctctct ccacttaatt cccgcatgct ttccgcattt gtgcatctgc cgaaacgaga
      781 gatgcccggc ttcagaatca ggaggtcggc agaattctct ctggctgaaa taccgccaaa
      841 agatccaaat cgagtcatcg ccatcggcaa cattctggac cgcggcgaag acaccggcaa
      901 cctctattac attgacgtag acgctctcca aaaacacacc atcgtctgcg gcgtcaccgg
      961 cggtggtaag acaaatactt gcttctacct ggtaggtcag ctctggcgtt acggaattcc
     1021 cttcatggtc tgcgagccgg ctaagtcgga ataccggcac atgatgctga tgtccgagac
     1081 cttcaaaggc gtcggtcagg ccttttctct tggtgacgaa accgtctctc ccttccgcct
     1141 caatcccttt gaaattatga aaggggtgaa agtacagact cacctggacg ctctcaaatc
     1201 agtgttcaac gccagttttg aaatgtactc gccgatgccg caggtactgg aaaaagccct
     1261 taactcgatc tatgccgtgc gtggctggga cttgatcaac aacgtcaacc gtcgtctgcc
     1321 gccgggcatg tccgtgggcg atcctgactg tcccaatgaa atttacccga ccatgaaaga
     1381 tctctacgag atcatcgatc ccatcgtgga gagcttcgga tattcagacc gcatcggtcc
     1441 cgacgtacaa gccgccctga aagccagaat aggctcacta ctaatcggcg gtaaggggca
     1501 gatgctcaat accaggcgct ccatacccat gggcgaactc ttcggcaaac cgaccgtggt
     1561 agagctgaag atggtttctg aggacagcga aaaatcattc ttgatgggta tgctcctggt
     1621 cttcctctac gaatacaggg aagcactcgg tccacacgat cacctgcagc acgtcatgct
     1681 ggtggaagag gctcaccgtt tgcttaagaa cgttccaacc ggtcagagcg gtgaatcggc
     1741 caaccctgcc ggtaaggctg ttgaattctt caccaacatg ctcgcagaaa ttcgttccta
     1801 tggtcagggc ttcatcatcg ccgaccaaat tcctaacaaa ctggcgcccg aagcccttaa
     1861 gaacaccaac ctcaagatca tgcatcgtat cgtggcggtg gatgaccgcg actctatggg
     1921 cggtgccatg aacttagatg atgtgcagaa aaggcacgtt acagctcttg gtcaggggcg
     1981 cgccctcgtc tatgcggaaa agatggagca gccttaccac ctgcaaatca tgttcgacaa
     2041 gaccaaggaa gtaccaccgc ccgaaacacc ggaagaatcc gacgaagtgg tcagacaggc
     2101 catgaagggt ctcaacatca tcggtagcta cgaccgtcac ctgggctgca aattttgctt
     2161 gcaccgctgc gattcgcaga ttctggatac ggctatccca gtggcggacg acccgatttt
     2221 ccgccaggtc tataaccgct atgtgctctc caccttgaaa gacctcaccc aacttgtgca
     2281 cttccgcgcc cagattattc atgaaattca gagggtgatc ggtggtcggg ctcgtaccgg
     2341 taatatcacc ggcatcacct ggtgcgctct cacccaggcc accgagcgtt acttcgagcg
     2401 taaaggtgaa aacaactact ggttctacga tcaggtgcgg gaacagcact tgc
//
LOCUS       DAOQNZ010000560         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25414_length_2450_cov_4.667223, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000560 DAOQNZ010000000
VERSION     DAOQNZ010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25414_length_2450_cov_4.667223"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..908)
                     /locus_tag="PKW73_10475"
     CDS             complement(117..908)
                     /locus_tag="PKW73_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73756.1"
                     /translation="MAPLMKAVTALFLVAAFTALLFRPAAASQTDVKAVLQKLDQLME
                     RENFAEALPLVNSLLKTHPGNARALRIRADIYSTTKKYKLALRDYDRLLAKPVPQGKD
                     YTHRGTVYLTMGDLVRAEADLLRGIELEQASPPAYRNLAEIYFKQGKKKEAEAVLSKM
                     VAAYPENLKTWRYKGEIDLRNGNGKAALADFDNCIRLSPMSHVYYLQKGDAQMLLKDY
                     KGALATYTKALEMEPFEVEEVYLKRAKAYEKLGMLKEAAADRARH"
     gene            complement(936..2240)
                     /gene="rodA"
                     /locus_tag="PKW73_10480"
     CDS             complement(936..2240)
                     /gene="rodA"
                     /locus_tag="PKW73_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNA73757.1"
                     /translation="MTTQTLDNLNFIPSALRTILRAFQGIDFWLLGATAFLIMVGLNV
                     LHFSQHTQGYFDKQLQYMWAGLALSLAVSRIPYKLWTNKYMVSFLYLLNLGLLVAVML
                     KGHSAQGAQRWLALGPITLQPSEVAKPIVIFSLAAWLRRFPINSFFDIFKILIILGPP
                     AVLVFKQPDLGTSLTFGAVFLGLSYWAGATITDILVLISPLISLILNAVNKEWWLYFL
                     GALFTILLFFWRRLDFKLWLRLLLIIAIVGANYGFGVARPHLWGVLKEYQQKRLTSFV
                     NPYDDPRGSGYHILQSLIAIGSGGVAGAGLGHGNQAQGAFIPERHTDFIFAVIGEELG
                     FKICALIVLAYGVICVRALFIALQSRGDPAGAAIAIGLMCMFLFHSFINIGMTIGIMP
                     VAGVPLPFLSYGGTALIVDLVSIGLLQSIYYHNPKEQKDIWG"
BASE COUNT          620 a          633 c          677 g          520 t
ORIGIN      
        1 catattctct aggggcgacg aaaaaagagc tgagcccagt gtcagagtac aacttctgaa
       61 agctcacctc gagagactca agcaactttc cgccgaaggt agcaagagcc cttgagtcag
      121 tgcctggctc tatctgcggc agcttcttta agcatgccca gtttctcata ggcctttgcc
      181 cgctttaaat agacttcttc tacttcgaag ggctccatct ccagggcttt ggtataagtt
      241 gcaagagccc ctttgtagtc tttcaggagc atctgcgcgt cgcccttttg caggtagtaa
      301 acatggctca tgggggaaag cctgatgcaa ttgtcaaagt cagccagggc ggctttaccg
      361 ttgccgttgc gcaggtcaat ttcgccctta tatcgccagg tcttcaggtt ctcaggataa
      421 gccgccacca tcttagaaag cacggcttcc gcttcctttt tcttgccctg cttgaaatag
      481 atctcggcca gatttcggta ggcgggcggg cttgcctgtt ccaattcaat gccccgcaaa
      541 aggtcggctt ctgccctgac cagatcaccc atggtcagat aaacagtgcc ccggtgggtg
      601 taatccttgc cctggggcac cggtttggct aagagtctgt cgtagtcacg caaagccagc
      661 ttatatttct ttgtggtgct atagatgtcg gctctgattc tcagggcgcg agcgttaccc
      721 ggatgggtct tcaaaagcga attgaccagc ggcagggcct cggcgaagtt ctcccgctcc
      781 atgagctggt caagcttttg cagaactgcc ttgacgtccg tctgagaggc cgccgcaggc
      841 ctaaacagga gggctgtgaa ggccgccacg agaaaaagtg ctgtaactgc cttcatcaat
      901 ggagccaaga atccatacct caagcccaca agagattaac cccagatgtc cttttgctcc
      961 ttggggttgt ggtagtagat tgactgcaag agtcctatgg ataccaggtc gacaataagg
     1021 gccgtgccgc cgtaactgag gaaaggcagg ggcacaccgg ctacaggcat gatgccgatg
     1081 gtcatgccga tattgatgaa agagtggaag agaaacatgc acatgagacc gatggctatg
     1141 gccgccccgg ctggatcacc tctggattgc aaggcgatga agagtgcccg cacacaaatg
     1201 acgccgtagg caagcacgat cagagcgcaa attttaaagc ccagctcctc gccgataacg
     1261 gcgaagatga agtcggtatg gcgctccggt atgaaggcac cctgggcctg gttgccgtgc
     1321 ccgagaccgg ccccggctac gccgccggaa ccaatggcaa tcagactttg caaaatgtga
     1381 tagccggaac cacgggggtc gtcgtaggga ttgacgaagc tggtcaggcg tttctgttga
     1441 tattccttga ggacacccca gagatgaggg cgcgccaccc cgaatccgta attggcaccg
     1501 acaatggcga tgatgagcag aagccggagc cagagcttga agtccaggcg gcgccagaag
     1561 aaaagcaata tggtgaaaag agcgcccagg aagtagagcc accattcttt gttgacggca
     1621 ttcaagatga ggcttatgag cggacttatc aaaaccagga tgtcggtgat ggtagcaccg
     1681 gcccagtaag aaagccccag aaagaccgcc ccgaaggtca aagaggtacc caggtcgggc
     1741 tgcttgaata caagcacggc cggtggtccc aggatgatca gtatcttgaa gatgtcaaag
     1801 aaagaattga taggaaagcg cctcagccag gccgccaggg agaagattac tatgggcttg
     1861 gctacttccg aaggttgcaa ggtaataggt ccaagggcca gccaacgctg cgccccctgg
     1921 gcggagtgac ctttcaacat aaccgccaca agtagcccta gattgagcag atacaagaag
     1981 ctgaccatgt atttgttggt ccagagcttg tatggaatac gcgaaacggc cagactcaaa
     2041 gcgagcccgg cccacatata ttgcaactgc ttatcgaaat aaccctgggt gtgctgagaa
     2101 aagtgcagca cattcaaacc caccattatt agaaaggcgg tcgctccgag gagccagaaa
     2161 tcgatacctt ggaaggcacg aagtatggtc ctcagagcgg agggaatgaa atttagattg
     2221 tccagggtct gggtggtcat aaaccaatcc ccaaggaact aaccaacaag agtttgttag
     2281 tccttagcac tttgatctga gcaggcggaa gttcgcgatt agcgaagtcc gacgctcata
     2341 ttgttgcaga tcgaactggg ttgtgtcttt atcgagttca aaataacgcg atacaaccgc
     2401 taccagttcc tcttccagac tctccaggcg accggccgga agctccagac
//
LOCUS       DAOQNZ010000561         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25444_length_2449_cov_4.151211, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000561 DAOQNZ010000000
VERSION     DAOQNZ010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25444_length_2449_cov_4.151211"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..>2449
                     /locus_tag="PKW73_10485"
     CDS             336..>2449
                     /locus_tag="PKW73_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA73758.1"
                     /translation="MQQIINAFIDAALKGRYLTLFASLVVVLAGIAAWKLLPIEAYPE
                     LANPQVRVITLTPGKGPEEVEKLITVPLEKEMNGIPGQIALRSLSLYGLSVIISTFTD
                     STPTTLARQQVLERIHAAALPPDITPHLEPDVGSLREIYRYCLHSPYYSTMGLRSIEQ
                     WEMEKLFRQIPGVIGVISEGGATKAYQINVDPYRLKAYGVTLKEVFEAAANSNATTGG
                     GFLEHNGSALIVRQLGLVRGIEDIKNVVIKATPEGTPVRISDVADVTIGSLVRRGQVG
                     KDYEDDVVEGIVLLRRGENPSQVLRAIKEKLPEILSHLPKGVQFSAMYDRDKLINQTL
                     HTVGTNVTIGITLVVVLLAVFLVDMGSAVITAIVIPLSMLIAFICLCLLGVPANLLSL
                     GAIDFGILVDSAVVMTENIVRRLSQDGQDMSPGERLILLSEAAREVGGPIVFGIITII
                     ATFLPIFTFSGVEGKLFRPLALTMVTALIGAGLAALTLVPVLMSFFLVKKPLKERTSP
                     VVALARFVYKPSLKWSLKHPLVVIACACAAIGYAGWLFTGIGSEFLPHLEEGNIWLRA
                     TVKPGSVTLAEGLKVARQVRTTLLQKYPEVKQVLSQVGGPDDGTDPAKFADQEHYVDL
                     KPAKEWRPQFHENKELLIASMRKELEQIPGVGYYFTQYIQTTLDEALSGVQGSLVAKI
                     SGPDLAQLETLGHKLGHIMGETP"
BASE COUNT          563 a          687 c          643 g          556 t
ORIGIN      
        1 cagtatctcg atgccgtacg cctttatcag gatgcttata ccgaactgga acaggcttgc
       61 ggcagacccc tggaatacta attgcagcac cggtaggcag tgcggaacca ggactaattc
      121 ttcttaattt tagccctgcc gcctcagaaa tcagagcccg catgtgccgt gaatggaggc
      181 aaaggggaca tttcatcaca acttaagcac atttggatgc aacaatatac ggaagcgtgg
      241 tctttgcccc cgagggctgt gattccgccg ctaaaagacc gaatccgatt ctccggcatg
      301 gcgcaacttt tcatcaaatt tagaccggga cctgcatgca gcaaatcatc aacgccttta
      361 tcgatgccgc actgaaaggc cgttacctga ctttgttcgc ctctctggtt gtggtgctgg
      421 ccggcattgc cgcctggaag cttttgccga ttgaagctta tcccgagctg gccaacccgc
      481 aagtgcgagt gataactctt acccccggca aaggaccgga agaggtagag aaactcatta
      541 ccgttcccct ggaaaaggaa atgaacggta ttcccgggca aattgccctg cgttcgcttt
      601 ctttgtacgg tctgtcggta ataatttcta ccttcacgga cagcacgccc acaaccctgg
      661 ccagacagca ggtgcttgag cgtattcacg ccgccgccct tccccccgat ataacccccc
      721 atctggaacc tgacgtgggt tccctgaggg agatttatcg atattgcctg cacagccctt
      781 actattccac catgggtttg cgctccatag aacagtggga aatggagaag ctcttccggc
      841 agatacccgg tgtaatcggc gtcatcagtg aaggaggcgc caccaaggcc tatcagatca
      901 acgtagatcc ttatcggctc aaagcctacg gcgttaccct gaaagaagtt ttcgaggcgg
      961 ctgccaattc caatgctact accggcggag gcttcctcga acataacggc tcggccctaa
     1021 ttgtcagaca gttaggtctg gtccggggca tcgaagacat caaaaatgta gtcatcaaag
     1081 ccacaccaga gggcacaccg gtgcgtattt ccgatgtagc cgacgtcacc atcggctccc
     1141 tggtacggcg cggtcaggtc ggcaaagact atgaagacga cgtggtggaa ggcattgtgc
     1201 tattgcgcag gggagaaaac ccctcccagg tcttgagagc tataaaggaa aaattgccgg
     1261 agattctctc gcacttgccc aagggcgtac agttttcggc tatgtacgac cgcgacaaat
     1321 tgatcaatca gaccctgcac acggtaggca ccaatgtcac gatcggcatc accctggtgg
     1381 tggttctgct ggccgttttt ctcgtggata tgggctcagc agtcattacc gccattgtca
     1441 ttccgctcag tatgctgatt gccttcatat gtctctgctt actgggagta ccggccaact
     1501 tactgtcgct gggagccatc gacttcggca ttcttgtaga cagtgccgtg gttatgaccg
     1561 agaacattgt gcgcaggctg agccaggacg ggcaggacat gtcaccgggc gaacgcctga
     1621 tcttgcttag tgaagcggca agagaagtcg gtggtccaat agtattcggc atcatcacta
     1681 ttattgctac cttcctaccc atcttcacct tttccggggt ggagggtaag ctcttccggc
     1741 ccctggccct gaccatggta acggcgttga ttggagccgg tctggccgct ctcacactgg
     1801 ttcccgtgct gatgtccttt tttctggtga agaaaccgct caaagaacgc acatcaccgg
     1861 tggtggcact agcccgtttc gtttataaac caagcctgaa gtggtcattg aaacaccccc
     1921 tggtcgtcat cgcctgcgcc tgcgctgcca ttggctacgc cggttggctt tttactggca
     1981 taggcagcga atttctcccg cacctggagg aaggcaatat ctggctgcgc gccacagtca
     2041 agccgggctc ggttactctt gccgagggtc taaaagtagc cagacaagtg cgcaccacct
     2101 tgttgcaaaa atacccggaa gtcaaacaag tactgtccca ggtgggcgga ccggacgacg
     2161 gtaccgaccc ggctaagttt gccgaccagg agcactatgt tgacctgaaa ccggccaaag
     2221 agtggcgccc gcagttccat gagaacaagg agcttctaat tgcatccatg aggaaggaac
     2281 tggagcagat tcccggagtc ggttattact tcacccaata catccagacc acgctcgacg
     2341 aagcgctgtc gggcgtacag ggctctctcg tagccaaaat ctccggtccc gatctggcgc
     2401 agttggaaac ccttggtcat aagctcggtc atatcatggg cgaaacgcc
//
LOCUS       DAOQNZ010000562         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25445_length_2449_cov_4.025063, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000562 DAOQNZ010000000
VERSION     DAOQNZ010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25445_length_2449_cov_4.025063"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKW73_10490"
     CDS             complement(<1..246)
                     /locus_tag="PKW73_10490"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA73759.1"
                     /translation="MGGRIIIVGGGFAGVKCARTLRKLLPPEWELVLFNRENHMVFHP
                     LLAEVVSATVQPKDVGAPLRQLLRGVICRAEEVTNILP"
     gene            464..1519
                     /locus_tag="PKW73_10495"
     CDS             464..1519
                     /locus_tag="PKW73_10495"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA73760.1"
                     /translation="MFEHLYFTDRPLSTLKSGYDLGGDAIDLSGSSVISFGRVFGEGE
                     NDDDVDYQFMSKCRPGENFAGCLDVEKEQLSGYPLFFRLLFDKLKDGSELLLFIHGYS
                     HSFEKLTGTVARLHRRYVKNPLSPIKHVLVFSWPSAGNVSRYAYDRKVVETSCAHFLE
                     FILELRRFARQCLTEEERGSFFDRINLMAVSMGNYLLERVLQLGQLESDTDATFHEVL
                     HMAADVDNRALHESGGLGKLAKMAKRVHVYFNRNDHLLLVSSGLHRRSRLGKDPHKES
                     LAPEALIIDTTDVSVKALMAPGQFFSKEEMDYATLHNYMLRVEAVVQDANRVLSHAPA
                     QAIDGRSYGGGSLFYLG"
     gene            complement(1530..>2449)
                     /locus_tag="PKW73_10500"
     CDS             complement(1530..>2449)
                     /locus_tag="PKW73_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNA73761.1"
                     /translation="FVVNSGSPDPTRSHFDAQDYMESGMPGNKVVSTGWLNRVLKELP
                     NNGSPVRALNVGATTPRILQGPIACATYAPTAKNRMNAMESPYIARAFEKMYNGRTDD
                     LGVAFAEGMEARATINEKLSDQMTAADQGALQANKFSGFGRQVGRLIKEEPKIQVAFV
                     DLGGFDTHVNQGASKGQLANNLNVVGKGLSELISSLGNTYEKTLIMVMSEFGRTVKEN
                     GNGGTDHGHGNFIWLLGGNVDGGKLHGNWSGLNDRALYEGRDLPVLTDFRSVLTDGIS
                     QHLGLSAKQTDAIFPGYSRKGAPIKVLKG"
BASE COUNT          498 a          618 c          696 g          637 t
ORIGIN      
        1 aggcagtatg ttggtcactt cttcagcccg gcagattacg ccccgcaaga gttgcctcag
       61 gggggcaccc acgtctttgg gctgcacggt ggcgcttacc acctcggcca gaagcgggtg
      121 gaagaccata tggttttccc ggttgaagag caccagctcc cactccggcg gcagcagttt
      181 cctcaatgtg cgggcgcatt tgacgccggc aaaaccaccg cccacaatga tgattcttcc
      241 gcccataatt acctcagctc aagagttgct taagggtatc ctggatgcct gggtttcttg
      301 gtcgggtttc tatattgatt cgacgcggcc tgccacttgt ttatattgtg gatggtagca
      361 taacggtctg tgaactgttt tcttctcgag agcatgcctc aagtagtcgc tatgatagaa
      421 gcatgacaga gtcgggattt tagaccagag gttggttgac caggtgtttg agcatctcta
      481 ttttaccgat aggcccttga gtaccctcaa gtcgggttat gacctgggcg gcgacgccat
      541 agatctgagc ggttcttccg tcattagttt tggacgggtt tttggtgagg gagagaacga
      601 cgacgacgtg gactatcagt ttatgtccaa gtgccgtccc ggcgaaaatt ttgcaggttg
      661 tctggatgtt gagaaagagc agctttcagg ctatccgctc tttttcaggc tgcttttcga
      721 caagctcaaa gacggttccg aactcttgct ctttattcac ggctacagcc attcctttga
      781 aaaactgacc ggcactgttg ctcgcctgca tcgccgctat gtcaaaaatc cgctttcgcc
      841 catcaagcat gtgttggttt tttcctggcc gtctgccggc aatgtcagcc gctatgccta
      901 tgatcgcaag gtggtggaaa catcttgcgc gcacttcctg gaattcattc tggagcttag
      961 acgtttcgcc aggcagtgcc tgacggaaga agagcgaggt tctttcttcg accggattaa
     1021 tctcatggct gtttccatgg ggaattactt gcttgagcgg gtcttgcagt tggggcagtt
     1081 ggaaagtgat accgatgcca ctttccacga agttttacat atggctgccg atgtagacaa
     1141 ccgggctttg catgaaagcg gcggtctggg aaagctggca aaaatggcta agagagtgca
     1201 tgtctatttc aatcgcaacg accacttgct tttggtttct tccggcttgc accggcgctc
     1261 gcgcctggga aaagatcccc ataaagagag tctggccccc gaggctctca ttattgatac
     1321 caccgacgta tcggtgaaag ccctgatggc ccccggtcaa tttttctcaa aggaagagat
     1381 ggattatgcc accttgcaca attacatgct cagggtggaa gcggttgtgc aagacgccaa
     1441 ccgggttttg agccatgcac cggcccaggc tatagatggc cgcagctatg gcggcggctc
     1501 acttttctat ttgggatagg gcaaagggat cagcctttga gaactttgat cggtgcccct
     1561 ttgcggctgt agccggggaa gatggcatcg gtttgtttgg ctgaaagacc cagatgctgg
     1621 gaaatgccgt cggtgagtac cgagcggaaa tcggttagaa ccggtagatc tcgaccttcg
     1681 tatagggcgc ggtcatttag acccgaccag ttgccgtgca acttaccgcc atcgacgttg
     1741 ccgcccagaa gccagatgaa gttgccatga ccgtgatcgg taccgccgtt gccgttttcc
     1801 ttcacggtgc ggccgaattc ggacatgacc ataatcaatg tcttttcgta ggtgttgccc
     1861 agggatgaaa taagttcact tagacccttg ccgacaacgt tcaggttatt ggccagttgt
     1921 cccttgctgg cgccttgatt gacgtgggtg tcgaagccgc ccaggtctac aaaggctact
     1981 tgaattttag gctcttcttt gatgagccga ccgacctgcc tgccgaagcc gctgaatttg
     2041 ttcgcctgca gggctccctg gtcggcagcc gtcatctggt cggatagttt ttcgtttatg
     2101 gtagctcggg cttccatgcc ttcggcaaaa gccacaccca gatcatcggt tcgaccgtta
     2161 tacattttct caaaggccct ggcgatgtag gggctctcca tggcgttcat gcgatttttg
     2221 gcggtggggg cataagtggc acaggcaatg ggaccctgca ggatgcgggg ggtagtggcc
     2281 ccgacattga gggctcgtac cggcgatccg ttgttgggta gttctttgag aacccggttc
     2341 agccaaccag tgcttactac tttgttgccg ggcatgccgc tttccatgta gtcctgggca
     2401 tcgaagtgag agcgggtggg atcgggggag ccgctattga ccacgaaag
//
LOCUS       DAOQNZ010000563         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25467_length_2448_cov_3.702466, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000563 DAOQNZ010000000
VERSION     DAOQNZ010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25467_length_2448_cov_3.702466"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKW73_10505"
     CDS             complement(<1..372)
                     /locus_tag="PKW73_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73762.1"
                     /translation="MPISNGASPEAVHPSRPERAANTFSKNLAGTVSEPRSRLFLALR
                     FAMAPRTYKRARKKSQPARSRLLDLLLLPPLLSLTICGTAAQALDGATVDKEIVARLE
                     KISKLQNSGDDFEKLEGANSDL"
     gene            complement(529..1323)
                     /locus_tag="PKW73_10510"
     CDS             complement(529..1323)
                     /locus_tag="PKW73_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73763.1"
                     /translation="MAKFIQLVLTITALGFCLAKAAPATQQFAFSPDRKHIATNSRGK
                     VTINNAQGKQLSQFDPKDSAGPHTGIMELYFVDNNRLGIRLHMNPSLDYLSITDLKGK
                     EIEHYLGYNFIWCNDRSAVAHVGQMIHFSEWPHSEYIQINNRTVYPPSGPAYGKEATT
                     VHTFIPPFCWSPDDSNLAVIDEVEGKDKGKYLVIIPTNLKVKGAGSSGKQAQTRRFQI
                     LPQQAASIGSQCPDGIKLTWQGKTAVTISAANGRQGKGLPCTISIK"
     gene            1518..>2448
                     /locus_tag="PKW73_10515"
     CDS             1518..>2448
                     /locus_tag="PKW73_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73764.1"
                     /translation="MSRIEMAEESQKSSSVSHLASDNLDLQVSNVNHLKDSSNAKSLL
                     DKQFGIPEIAGLDQLESKAPMKLAGSQIGSLNRLDSSVGSSDATGKLDFGDNSIFKSV
                     FSSSGDNSILLAQNVRLFEGLSPAVQKHYDAIRNGVSDAFNEPFANRTKGLNGLEIVK
                     DLTVGGYLGKNTNEPPMFDDAYSAKVTRPYEQRLMKDLQADQGPISHKTMVERAMYIC
                     NNDKYLATLTLANFTKNIASVERGQAPVTDRPRPDMGYPFDTFSKQQSDNVFNRLERF
                     DQNSPDKMSKTDVAGTLYHFYGGILASSAGLGGG"
BASE COUNT          565 a          581 c          656 g          646 t
ORIGIN      
        1 cagatcgcta ttagcgcctt cgagtttctc gaaatcgtcg ccggagtttt gtaatttgga
       61 aatcttctcc agccgggcga caatttcctt atcaacggtg gcaccgtcca aagcctgggc
      121 cgctgttccg catattgtca gagacagcag gggcggcaga agcaagaggt caagcagcct
      181 ggagcgtgcc ggttgcgatt tttttcttgc tcttttgtag gtgcgcggtg ccatggcaaa
      241 gcgcagagcc agaaaaagtc ggctgcgcgg ttctgagacg gttccggcaa ggttcttgct
      301 gaatgtattt gcagctcttt cgggacggct cgggtggaca gcttccgggg aagctccatt
      361 tgatattggc atctcaatac tgacctttgc tgaaaaaaat tctgaccttt acttctgagc
      421 cccgtggttt ccattttatg gtgatgagtc ggccagttta tcttgtggcg gtttacttgt
      481 aaattcaggt gaggaaatcc atggtgttaa gtccgtagtg tgaagctttt acttgattga
      541 tatggtacag ggcaagcctt ttccctggcg gccgtttgcc gcagagatag tgacagccgt
      601 ctttccctgc caggtcaact tgataccgtc gggacattgc gaaccgatgg aagcggcttg
      661 ctgcggcagt atctggaagc ggcgcgtctg cgcctgcttg ccggagctcc ctgcgccctt
      721 caccttcagg ttagttggaa taatgaccag gtacttgcct ttatccttcc cctcgacttc
      781 atctattact gccaggttgc tgtcgtcggg actccagcag aagggtggga tgaaggtgtg
      841 caccgtggtt gcctctttac cgtaagcggg tccggaagga ggataaactg tgcggttgtt
      901 tatctggatg tattcactgt ggggccattc gctgaagtga atcatttgcc cgacatgagc
      961 taccgccgag cgatcattgc accagatgaa gttgtaaccc aggtagtgtt ctatctcttt
     1021 gcctttcaaa tctgttattg aaaggtagtc cagtgatgga ttcatgtgca gcctgatgcc
     1081 caggcggttg ttgtctacaa agtagagttc catgatgccg gtgtggggac cggccgagtc
     1141 tttaggatca aattgggaga gttgcttgcc ctgggcatta ttgatcgtga ccttacccct
     1201 tgagttagtg gcaatgtgct tccgatccgg tgagaaggca aactgctgag tggctggggc
     1261 ggcttttgcc agacagaagc ctagagccgt tatggtgaga accagttgta tgaatttagc
     1321 caaggtgatt ttcgaaaaga ttatgggtga cgggcaaacc tcagtttaca acctctgaca
     1381 cggtaggtca tgaccgctgg tatgtacttg tcacagagtt tgccataaat tcatattgtc
     1441 aagcgctgac tggctttgag tgcctggtcc ccttactagc aacaattaag cgccaatatt
     1501 cccctgaggt gccgtatatg tccagaatag aaatggcaga agagtcgcag aagagctctt
     1561 ccgtctctca cctagcttcc gacaatttgg atttacaggt ttcgaacgtc aaccacttga
     1621 aagatagttc gaatgccaag tctttgctcg ataagcagtt cggcatacct gagatagccg
     1681 gcttggacca gttggaatcc aaagccccga tgaagcttgc cggcagccag atcggctctc
     1741 taaaccgcct ggactcttca gtgggttctt ctgatgcgac cggcaaactc gactttggcg
     1801 ataacagtat tttcaaatcg gtgttttctt cttccggtga caattcaatt ttgctggctc
     1861 aaaatgttcg cctatttgaa gggctttcac cggcagtgca aaagcattat gatgccatcc
     1921 gtaatggagt atccgatgca ttcaatgagc cgtttgcaaa tagaacaaaa ggattgaacg
     1981 gactggaaat tgtgaaggac ctgacagttg gtgggtatct gggcaaaaat accaacgagc
     2041 cgccgatgtt tgacgatgcc tacagtgcca aggtaaccag accttacgag cagcggttga
     2101 tgaaagatct gcaagccgac cagggtccga tcagccataa gacgatggtt gagcgagcca
     2161 tgtatatttg caacaatgac aaatatctcg ccacgctcac cctggctaac ttcaccaaga
     2221 atattgcttc tgtagaacga ggccaggcac cggtgaccga cagaccgcgg ccggacatgg
     2281 gctacccctt cgacactttc tccaagcaac agtcggataa cgtcttcaat agactggaaa
     2341 gattcgatca gaattcaccc gacaaaatgt ctaagaccga tgtggcggga actctctatc
     2401 atttctacgg tggaattctc gcatcctctg ccggtcttgg tggcgggg
//
LOCUS       DAOQNZ010000564         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25471_length_2448_cov_3.065608, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000564 DAOQNZ010000000
VERSION     DAOQNZ010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25471_length_2448_cov_3.065608"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PKW73_10520"
     CDS             complement(<1..121)
                     /locus_tag="PKW73_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNA73765.1"
                     /translation="MGDFKQTMEAISGSAANIAKILKTIDEIAFQTNILALNAA"
     gene            149..550
                     /locus_tag="PKW73_10525"
     CDS             149..550
                     /locus_tag="PKW73_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73766.1"
                     /translation="MGVAGKLLRLGRGVGVALSHARDLLKRGGGFFQRRSLLAPPRTR
                     VLGFGDAEALLNIIENTQERRSRELEKRSREREQTALSESTGARSYCPRSTNLDLQAL
                     ARPGVLKKALQKAFKAAKREMVKLLDKLALA"
     gene            671..853
                     /locus_tag="PKW73_10530"
     CDS             671..853
                     /locus_tag="PKW73_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73767.1"
                     /translation="MLKSEAERVVRKAMEETEAQFTEEQIQAISQICLKIAATVVEEA
                     FASWRPSSGGKPNFFA"
     gene            936..2186
                     /locus_tag="PKW73_10535"
     CDS             936..2186
                     /locus_tag="PKW73_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNA73768.1"
                     /translation="MAKSKENAAAAAGTSSVSTAGTVDRHFLHQAKSLEEKLIEMRRH
                     LHEYPELSFHEHKTAALMKDKLDSYGYKTKAGVGKTGVIGDLGKDGPVIAIRADMDGL
                     PIDETNPNKYISKNKTVMHACGHDGHMACGLTAAQMLSQTELPGRVRMVMQPAEEATD
                     SDGKSGAWRMLEDNALDGVSAIIGLHADASLKAGQVGIISGPAMAACDGFKVTIKGKG
                     GHGAYPETTVDPVVIGAQVVQAIQQIVSRRVSALEPAIITIGAFHSSSTRGNIISDYV
                     ELEGSFRSFSQAVREHIIAELDKACSIARALGGDYKIDYELGYPCTVNHPEITEVMRQ
                     AAIDLIGKENVIAVQPKTWSEDFSMFAEKVPGAFMFLGAEIENDTRSHHSPEFDLNES
                     GLYLGSAILAETARRLMQHYKDKQ"
     gene            2261..>2448
                     /locus_tag="PKW73_10540"
     CDS             2261..>2448
                     /locus_tag="PKW73_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNA73769.1"
                     /translation="MNPGLDQLQQYPFERLNALKAGLTPPPVSPIVMAIGEPKHEPPG
                     FVLDALRAVASRHGVSVAR"
BASE COUNT          598 a          722 c          675 g          453 t
ORIGIN      
        1 cggcggcgtt caaggcgagg atgttggtct ggaacgcgat ttcatcgatg gtcttgagaa
       61 tcttggcgat gttggcggcc gagccgctga tcgcctccat cgtctgcttg aaatcgccca
      121 tttcctgcgt gcccttgcgg gcggcatcgt gggcgtcgct ggaaagctgc ttcgcctcgg
      181 ccgcggcgtc ggagttgcgc tgagtcatgc tcgcgacctc ctcaagcgag gcggaggttt
      241 cttccagcgc cgcagcctgc tggcacctcc tcgcaccaga gtactcggtt tcggcgacgc
      301 cgaagccctc ctcaacataa tcgaaaatac acaggaaagg cgcagccgtg agctggaaaa
      361 acgcagccgc gaacgcgagc aaacagccct gagcgagagc accggcgccc ggagctattg
      421 cccccgcagc accaaccttg atctgcaagc cctggccaga ccgggagttc tcaagaaagc
      481 tctgcaaaag gccttcaaag ccgccaaaag agaaatggtc aaacttctcg acaagctcgc
      541 cctcgcctga gcctgggcat tcacaacaat tcaatcgctt ttcgcgctaa actttgaggc
      601 aatcaaggcg gaaaatcagg ctgcacggtt gtcagtcgag aaccgccttc tgtctgacag
      661 ggagatagcc atgctgaaat ctgaagcgga aagagtcgtg cgcaaagcaa tggaagaaac
      721 cgaagcccag tttacggaag aacagatcca ggctattagc cagatttgtt tgaagattgc
      781 ggctaccgta gtggaagaag ctttcgcttc ctggcgtccc agttcgggcg gcaaacccaa
      841 cttcttcgcc tgaaaacaag aacccacggc gggtctttca ccattcggcg gccgatttta
      901 atttagagca gacgttttac ttcgagaaat ttccaatggc caaaagcaaa gaaaacgcag
      961 ccgctgctgc aggcaccagc tcagtcagca cggccggcac cgtcgaccgt cattttctcc
     1021 accaggctaa aagtctggaa gaaaaactga tcgaaatgcg tcggcacttg catgagtacc
     1081 cggagttgag ctttcacgag cataaaaccg ccgctctcat gaaggataag ctcgactcct
     1141 acggctataa aaccaaagcc ggcgtgggca aaacgggagt gatcggagat ctcggcaaag
     1201 acggaccggt gattgccatt cgcgccgaca tggatggtct ccccatagac gaaaccaacc
     1261 ccaataaata catctccaaa aacaagaccg tcatgcatgc ctgcgggcac gacggtcata
     1321 tggcctgcgg tctgaccgcc gcgcagatgc tttcgcaaac ggagttgccg ggtcgggtgc
     1381 gcatggtcat gcaaccggcc gaagaagcca ccgattccga cggcaagtcg ggggcctggc
     1441 gcatgctgga agataatgcc ctggacggtg tctcggctat catcggcttg catgccgacg
     1501 ccagcttgaa agccggtcag gtgggcataa tttccggtcc ggccatggcc gcttgcgacg
     1561 gcttcaaggt gaccatcaag ggcaaaggtg gtcatggcgc ctatcccgaa accacagttg
     1621 atccggtcgt gatcggcgcc caggtggtgc aggctattca gcaaattgtt tcaagacgag
     1681 tctcagccct ggaaccagcc atcatcacca tcggggcctt tcactcttcc agcaccaggg
     1741 gtaatatcat ttccgactat gtcgaactgg aaggcagctt ccgcagcttc agtcaggcag
     1801 tgcgggaaca tatcatcgcc gaattggaca aggcctgctc gattgcccgg gctctgggag
     1861 gcgattacaa aatcgactac gaactgggtt atccctgcac ggtcaaccac ccggaaataa
     1921 cggaagtgat gcgacaggcc gccattgacc tgatcggtaa agaaaacgtt attgccgtgc
     1981 agcccaagac ctggtcggaa gacttctcca tgtttgcgga aaaggtaccg ggagctttca
     2041 tgttcctggg cgccgaaatc gaaaacgaca ccagaagcca ccacagcccc gaattcgatc
     2101 taaacgagag cgggctttat ctgggctcgg ccattctggc agagacagcc agaaggctca
     2161 tgcaacacta caaagacaag caatagcaag ccgcacccaa gcgccggtcc aatgcgggaa
     2221 aagcgcagcc agaaccagag ccaaatccag aaccaacttt atgaatcccg gactcgatca
     2281 actccaacag taccccttcg aaagactgaa tgccctcaag gccggactga cgccgccgcc
     2341 ggtcagccct atcgtcatgg ccatcggcga gcccaagcac gagccgcccg gcttcgtgct
     2401 cgatgcgctc cgcgcggtcg cctcgcgtca cggcgtgtcg gtcgcgcg
//
LOCUS       DAOQNZ010000565         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25486_length_2447_cov_3.578595, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000565 DAOQNZ010000000
VERSION     DAOQNZ010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25486_length_2447_cov_3.578595"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2447)
                     /locus_tag="PKW73_10545"
     CDS             complement(<1..>2447)
                     /locus_tag="PKW73_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA73770.1"
                     /translation="SGNDGTSNINVTFDLERDVDLAAVDIQNRISAAQGRLPEEVKRT
                     GVQVNKVSNSFVLAIGILTPDNRYSSQFLSNYANIYIRDALKRVKGVGEVRIFGERKY
                     SMRVWLDPHRLAVKGITAQDVVTAINDQNLQVAAGQIGQAPLPENQMFQMSVRGVGRL
                     KTAQEFENIVIRSGKDGQLVRIKDVGRAELGAEDYQNVVRYHGKDAVGIGIFQRPGSN
                     ALEISNGVREEMTRLSQRFPPGIAYEFAFDTTRSVKESIKEVLITLLQAIALVVLVIF
                     LFLQSFRSTLIPVFTIPVSLIGTFAIMKVLDFSINTLTLFGITLATGLVVDDAIVVIE
                     NITRLMHERNLTAREAATLAMREVTGAVIAISLVLSAVFIPVAFFPGTTGQLYKQFAL
                     TIAISVAISTLNALTLTPALSALWLKKGSLTGSIIFKPVNFVIDFFRNIYSATLGLVL
                     KLKYLVLVGFAGLLCCTYWLSKTVPTAFIPEEDVGYLIIIVQAPEGVSLNYTVNVIKK
                     IEKVLDNCPEIVSSFGVGGFSFTGTNPNNAIVFSNLKDWHERKGYEHSLKGIIERLRG
                     PLGGITEATVIPFNPPSIQGLGNFGGFAFELQDLFGTDIGQLAGVTRQIMMEAAGEKQ
                     LTGVFSPFTANSPQLITEVMRDKARALKVLVSDVFQTMEIMLGSRYVNDFDLGNRIYR
                     VYVQADELFRSNPKDIGGLYVRSQNGEMISLSHLVKVERTTAPQTISHYNLFRCAEVN
                     GSAAPGVSSGQALTAMERLAKRILPQGMSYEWSGISLEEKESGPQTLILFALGIVVVF
                     LVLAAQFESLTLPLAVI"
BASE COUNT          631 a          652 c          637 g          527 t
ORIGIN      
        1 aatgacggcg agcggcagcg tcagactttc gaactgggcg gcaagcacca ggaaaaccac
       61 gacaatcccc agagcaaaaa gaatcaaggt ctgaggacca ctttcctttt cctcgagaga
      121 aatgccggac cattcataac tcatcccctg aggcaaaatc cgcttggcca gccgctccat
      181 ggccgtaagc gcctgaccgc tggaaactcc gggagcagcc gagccgttca cctcggcaca
      241 gcggaagaga ttgtaatggg atatggtctg cggtgcagtg gtgcgctcta ccttcaccag
      301 gtgcgaaaga ctgatcatct cgccgttctg cgaacgcaca taaagcccgc ctatatcctt
      361 ggggttggag cgaaagagtt catcggcctg aacatacact cggtagatgc gattgcccag
      421 atcgaagtcg ttcacatatc tggagcccag cataatttcc atggtctgaa aaacatcgga
      481 cacaagcact ttcagggctc tggccttgtc gcgcatcact tccgtgatga gctgcgggct
      541 gttagccgtg aagggtgaaa atactcccgt caactgtttc tcgcctgccg cttccatcat
      601 aatctgcctg gtaacaccgg ccagttgacc gatatcagta ccgaagagat cttgcaattc
      661 aaaggcaaaa ccgccgaagt tgcccagacc ctgaatcgat ggagggttga atggaatgac
      721 cgtagcctcg gtgatgcctc ccagaggtcc ccgcaatctt tcaatgattc ccttgaggga
      781 atgttcataa cctttgcgct catgccagtc tttcaaatta gaaaagacaa tggcattgtt
      841 gggattggtg ccggtgaagc tgaagccgcc tacgccgaag gaagaaacta tctcaggaca
      901 gttatccagc accttctcta ttttcttaat tacattgacg gtgtaattta gcgaaacgcc
      961 ctccggcgct tgcacgataa tgatcaaata gcccacatct tcctcaggaa tgaaagccgt
     1021 gggaacggtt ttagagagcc agtaagtgca acagagcagc ccggcaaagc ccaccagaac
     1081 aagatatttg agcttcaaaa ccaagcccag ggtggcactg tagatgttgc gaaagaaatc
     1141 gatcacgaaa ttgaccggct taaagatgat actgccggtc aatgaacctt tcttcaacca
     1201 gagcgccgac agagccggcg tcaatgtgag cgcattgagg gtggatatgg ccaccgatat
     1261 ggcaatggtg agggcgaact gtttgtagag ctgaccggtg gtgccgggga agaatgccac
     1321 cggtataaat accgcagaca gcaccagtga aatggcaatg acggcgccgg tgacttctcg
     1381 catggccagg gtggcggcct cccgggccgt caagttgcgc tcatgcatga ggcgcgtaat
     1441 gttttcgatg accacgatgg cgtcatccac aaccaatccg gtagcgagag tgataccaaa
     1501 tagggtgagt gtattgatgg aaaaatcgag caccttcatg atggcaaagg tgccaatcaa
     1561 agacacagga atcgtaaaga ccggaatcaa agtagagcgg aaactctgca agaagaggaa
     1621 aattaccagc accacaagag cgatggcctg cagcaaagta atcaatactt ccttaatgga
     1681 ttccttgacc gagcgggttg tgtcaaaggc aaattcataa gcaatcccgg gcgggaagcg
     1741 ctgggacaaa cgggtcatct cttctcttac gccattggat atttccagag cattggaacc
     1801 agggcgctgg aaaataccga tgcccaccgc atctttgccg tggtagcgca ccacattctg
     1861 gtagtcttcc gctcccagct cagcccttcc cacgtcctta atgcgcacca gttgcccgtc
     1921 tttaccagag cgaatgacaa tgttttcaaa ttcctgggcg gtcttgaggc gaccgacgcc
     1981 ccgcacactc atctggaaca tttgattttc cggcagcggt gcctgtccga tttgaccggc
     2041 ggccacctgc aaattctgat cattgatggc cgtcaccaca tcctgggcag tgattccttt
     2101 cacagccaga cggtgcggat caagccagac ccgcatggag tatttgcgct cgccgaaaat
     2161 tctaacctcg cccacaccct tcacacgctt gagggcatcg cgaatataaa tattggcata
     2221 attgctgagg aactggctgg agtagcgatt gtccggcgtc agaataccga tggccagcac
     2281 aaaggaattg gaaactttat tgacctgaac accggtgcgc ttcacctctt ccggtaaacg
     2341 cccctgggca gccgagattc tgttttggat gtccacggca gccaggtcca catcccgttc
     2401 cagatcgaag gtgacgttga tattggaagt gccgtcgtta ccgctgg
//
LOCUS       DAOQNZ010000566         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25531_length_2444_cov_4.365425, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000566 DAOQNZ010000000
VERSION     DAOQNZ010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25531_length_2444_cov_4.365425"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKW73_10550"
     CDS             <1..642
                     /locus_tag="PKW73_10550"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA73771.1"
                     /translation="GVLEHLPAGSVVVDFSTIGPETARRLADRFQASGSCFLDAPVTG
                     GDVGARNGTLTIMVGGDAIAFERVRPYLSLMGKTLVHCGVSGSGQALKLCNQVLCSVN
                     LVALLEAMTLARRLGLDESLVVEALGNGAGGSWALSNLGPRILKRDFAPGFSIRHVLK
                     DLRLVGESLNEGGFGVLGGTQWSVQRFNQVVEEGGAAALDLGTQAMYLAYEDS"
     gene            871..1077
                     /locus_tag="PKW73_10555"
     CDS             871..1077
                     /locus_tag="PKW73_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73772.1"
                     /translation="MSTATATVMDTALLDEVVHWVKTLTAAGVSPDAAAKVTTKILLA
                     ANSEESDDFWCEGDEDLDDMDDEE"
     gene            1185..>2444
                     /locus_tag="PKW73_10560"
     CDS             1185..>2444
                     /locus_tag="PKW73_10560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA73773.1"
                     /translation="MPANQEIKTTDGLPTPALSEDSPPVDLVLSYQTGNFVATRLLLL
                     ALSPLWFAWSLFCLVGLFTEGGKAGWLVDFNQMAAAAFCYASLLALSFFALRVCSDTR
                     LHLGENGVRLPLRFLLESRGRLKRSWSELVQVDFLEDGNLRTQVSTMTLVFQDGARVP
                     LRLAGLGRAALKNLLLIFSIKAPSVTFRPELSQVDLSLPVGAVSASGLGLHPSYTQLW
                     EEEMASRFGSTSFVPLKPGDKLRDGQIQITGQLGFGGLAAVYLATLQPAGRCVVVKEA
                     VLPLGVEAALKEKAVSLFQREAEILSQVRHKRIVSIEDNFQENDRHYLVLSHVDGIDL
                     RRFIKQEGAIKSVVAARWLFDLSSVLVYLHGLERPVLHRDLTPDNVVITSDGELVLID
                     FGVAASFVGEATGTLVGKQSYIPPEQYR"
BASE COUNT          499 a          609 c          717 g          619 t
ORIGIN      
        1 ggggtgcttg agcatttgcc ggccggttcc gtggtggttg acttcagcac aatcggtccg
       61 gaaacggcca ggcggcttgc ggaccgcttc caggcatccg gctcttgttt tctggatgct
      121 ccggtgaccg gcggcgatgt gggggcgcgc aacggcactc tcaccatcat ggtcgggggt
      181 gatgcaattg ccttcgagcg ggtgcgaccc tacctcagct tgatgggcaa aaccctggta
      241 cattgcgggg taagcggtag cggtcaggct ctcaagcttt gcaaccaggt gctctgctcc
      301 gttaatctag tggccctttt ggaagccatg accctggctc gccgccttgg actggacgaa
      361 agcctggtgg tggaagccct tggcaatggc gcgggcggct cctgggcttt gagcaacctt
      421 ggcccgcgaa ttctcaagcg ggattttgcc ccgggctttt ccattcgcca tgtgcttaaa
      481 gatttgcgac tggttggaga aagtcttaat gagggtggtt ttggggtgct gggcggcact
      541 cagtggtcgg tgcaaagatt caatcaggtg gtggaagagg gcggagcggc ggctctcgac
      601 cttggtaccc aggccatgta tctggcctat gaggactctt aaggggacag tcgattactt
      661 agggcaccgg tgggagtttg cagctatctg gcaaccagca tttcaataca tatgaatgaa
      721 gtatatacgg ttgtcacggc agagaattta attcaaaagc agtacacaag ccagttaagg
      781 tggggtagca tttattgtct agtcaatacc tgtctcctgc tccttggggc tggtgttgtt
      841 cgtttgtgag cctagagagg gaattttaga atgtctacag caacagctac agtaatggac
      901 acagccttgc tcgatgaggt tgtgcactgg gtaaaaaccc tcaccgctgc cggcgtctct
      961 cctgacgctg ccgcaaaagt gaccactaag attcttcttg ccgccaattc ggaagagtct
     1021 gatgatttct ggtgcgaagg cgatgaagat ctcgatgata tggacgatga agagtaatcg
     1081 cttcacgtcg taaatctact tgatgaaagc tccccggccg accggggagt ttttgtttgg
     1141 caaatcaggt aaaagataag tagaagatcc tggggagatt tatcttgcct gccaatcaag
     1201 aaatcaaaac tacagacggg cttcccaccc cggcgctgtc tgaggacagt ccgcccgtag
     1261 acctggttct ttcttatcag accggtaact ttgtagccac ccgcctcctc cttctggcac
     1321 tctcgccgct ctggtttgcc tggtcgctgt tctgcctggt cggattattt accgaaggtg
     1381 gaaaagccgg gtggttagtt gatttcaacc agatggcggc ggcggcattc tgctatgcga
     1441 gtttgcttgc actcagcttt tttgccctgc gggtttgctc cgataccagg cttcatctgg
     1501 gagaaaacgg tgtgcggctg cctttacggt tcttgctcga aagccggggg cgactaaagc
     1561 gttcatggtc ggagcttgtt caagtagact ttcttgaaga cggcaacctc cgcacccagg
     1621 tcagtacgat gactctggtt ttccaagacg gtgcgcgggt acccttgcgt cttgccggtc
     1681 tgggacgagc tgccttgaag aacctgcttc tcatcttttc catcaaggcg ccctctgtca
     1741 cttttcgtcc cgaactatcc caggtggatc tctctttacc ggtaggagcc gtatctgctt
     1801 ccggtcttgg tctgcatccc tcctatacgc aactctggga agaagagatg gccagccgtt
     1861 tcggctcgac cagttttgtg cctctcaagc ccggcgataa gttgcgtgat gggcagattc
     1921 agattacagg acaacttggc tttggcggac tggcagcggt ttatctggct acgcttcaac
     1981 cggcaggacg ctgcgtagta gtgaaggaag cggtgctgcc cctgggagtg gaggcggcgc
     2041 tgaaagaaaa agccgtgtca ctgttccagc gcgaggctga gattttgagc caggtccggc
     2101 acaagagaat cgtgtctatt gaggataact ttcaagaaaa tgacagacat tacctggtgc
     2161 tctcacatgt ggacggcatc gacttgcgcc gcttcatcaa gcaggaaggc gcgataaaga
     2221 gcgtggtggc agctcgctgg ctctttgacc tgtcctcagt gctggtttat ttgcatggac
     2281 ttgaacgacc ggttttgcac cgagatttga ctcccgacaa tgtggttata accagtgatg
     2341 gggagttggt tttgatagac tttggcgtgg ctgcaagctt tgtgggagaa gccaccggca
     2401 cccttgtcgg caaacaatct tacataccac ctgagcaata ccgg
//
LOCUS       DAOQNZ010000567         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25588_length_2441_cov_3.763202, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000567 DAOQNZ010000000
VERSION     DAOQNZ010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25588_length_2441_cov_3.763202"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="PKW73_10565"
     CDS             complement(<1..1961)
                     /locus_tag="PKW73_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA73774.1"
                     /translation="MTVDAACDIFSGSSSEELAAGQVVAGTYRVLSLIARGGMGNVYL
                     VEHIYLLKTFAMKVLPAYLAGAEASRRFLAEARSIARLEHENIVKIHNFGLTDAGVSF
                     YVMELVDGLCLEDWLLLNGPVTIEQTLNLMHCLCRGLGYAHGRGFVHRDIKPANVMVD
                     FRGAYPRVKLLDFGLVQCSQNATGQERCFGSPLYMSPEQCEGLSVDRRTDVYSLGCLF
                     YACLTGVPPFMGSNPLATCYMHTNGARPVLRQEQLREPVDADTLHKLNQILQFLLSVR
                     REERYRDMDEVLLDLSLIGSEPEPEEPTEPETIAEEKVFTGWLVAFLCVLGTASVLLS
                     LWFSLQCFEGARAESRRQKVDSSVAALVDSTERNELDIQEMEDLKPFMDGSGKFRHFA
                     FPRTACLGILRAADDANLLMKAQGELTFPGQSPLIFHPSAACLNKPRYLRRFSSGQLE
                     GLYAAGNELLDDDNLRYSDHLKDLKYLNCHFTRLSDASLTTIAKFDQLQFLDVSHTLI
                     SHLESLPNLRNLKTLYMDSLPGLPAVLKELRKSSVIEALDISDISIAASDVELLKGLK
                     KLRRLNLHGSKIDPERLPALLDSLSNLSYLDLSANELSDAQVERLLPALKRSHLRILL
                     LSKDGLSEARRHRIESNLPDACRVNYETV"
     gene            complement(2095..>2441)
                     /locus_tag="PKW73_10570"
     CDS             complement(2095..>2441)
                     /locus_tag="PKW73_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73775.1"
                     /translation="GLNANERARVALCLSRIEALLAESPQWQPDEFPNEVLIEEEPLW
                     SALRAEAAICLAEVDFSLSEWEEEQDSILIELLISPRLQTQLAALQRERKQSQHLLIN
                     DILQEWLASKGR"
BASE COUNT          678 a          664 c          583 g          516 t
ORIGIN      
        1 atgaccgtct cgtaattcac acggcaagca tccggcaaat tactctcaat tcggtggcgc
       61 cttgcttcgg aaagtccgtc tttagacaac aagagtattc tcaaatgaga ccgcttcaaa
      121 gccggcagca gtcgctcgac ctgagcgtcg ctcaattcgt tggcagaaag atccaggtaa
      181 ctcaaattac tgaggctatc aagcagagcc ggcagcctct ccggatcaat tttagaacca
      241 tgcaagttga ggcgccgcaa tttcttcaaa cctttcaata attccacatc gctagcggca
      301 atactgatat cgctaatgtc gagtgcctca atcactgaac tcttacgcaa ctccttcaac
      361 accgccggca aaccaggcag agaatccatg tataacgtct tgagattcct gagattgggc
      421 aagctctcca aatgagaaat aagcgtatga ctgacatcta gaaactgcaa ctggtcaaac
      481 ttggcgatag tagtaagaga tgcatcggaa aggcgagtaa agtgacaatt gagatacttg
      541 aggtctttaa gatgatcgct gtatcgcaaa ttatcatcat ccaaaagttc gttaccggcg
      601 gcatagagcc cctccagttg ccccgaagaa aagcgcctga gatagcgcgg cttattcaga
      661 caggcagcgg aaggatgaaa gatgaggggg ctctgacccg gaaaggtcag ctctccctgg
      721 gccttcatta agaggttcgc atcatcggca gcccgcaata tgccaaggca agccgtgcgc
      781 ggaaaggcaa aatgcctgaa tttcccactt ccatccatga atggcttgag atcttccatt
      841 tcctgaatat caagctcatt tcgctccgtg ctatccacaa gtgccgccac agagctatcg
      901 accttctgcc tgcgactttc agcccttgct ccttcaaagc attgcaggga aaaccagagg
      961 ctaagcaaaa cagaagcggt accgagcaca cagagaaatg ccacaagcca gccggtaaag
     1021 actttctctt cggcaatggt ctcaggctca gtcggctcct caggctccgg ctcggacccg
     1081 atcaaagaaa gatccaacag aacctcatcc atgtctctat agcgctcttc ccgacgtacc
     1141 gaaagcaaga actgaagaat ctgattaagc ttatggagag tatctgcatc aacaggctcc
     1201 cgcagctgct cctgccgcaa gaccggtctt gctccattcg tatgcatgta gcaggtggca
     1261 agtgggttcg agcccataaa aggcggcact ccggtgaggc aggcatagaa caaacagcca
     1321 agcgaataga catcggtgcg ccggtccact gagagacctt cacactgctc tgggctcata
     1381 tataaaggac taccgaaaca acgctcctgc ccggtggcat tctgactgca ctgcaccagg
     1441 ccaaaatcca gaagcttgac ccgggggtaa gcgccgcgaa aatctaccat gacattggcc
     1501 ggcttgatat cccgatgcac aaagccccta ccgtgggcat aacccagccc ccgacaaaga
     1561 cagtgcataa gattcaaagt ctgctcaatc gtaaccggtc cgttcaataa aagccaatct
     1621 tcgaggcata gaccatcaac aagttccata acatagaaag agacgccggc atcggtaaga
     1681 ccgaagttgt gaattttgac gatgttctca tgctccaggc gagcgataga acgagcctca
     1741 gccaggaagc ggcgagaggc ctccgccccg gccagatagg caggtaacac cttcatggca
     1801 aaggtcttca acaaatagat atgctcgacc aggtagacat tacccatgcc gcctctggca
     1861 atcaagctca gcacccggta agtaccggcc acaacctgac cggcagctaa ttcttcactg
     1921 cttgaacctg aaaaaatatc gcaggccgca tcaacggtca taggaccaaa ccgccttaag
     1981 aaaatgaatt tagtaacggg ctcaatggta aaggctgccc agtgaaagtg tcgtgaaagt
     2041 gctaaaggag gcgagctggc attggagacg gcgcctcaac tctgacagga acctttaacg
     2101 gcctttggag gcaagccatt cttgaagaat atcatttata agtagatgct gtgattgctt
     2161 acgctctctc tgtaatgcgg caagctgtgt ttgcaatctg ggtgaaatga gcagttcgat
     2221 tagaattgag tcctgctctt cttcccattc actcaatgag aaatcaactt ccgccaggca
     2281 gatcgccgcc tccgcccgaa gggcagacca caaaggctct tcttcaatca gaacttcatt
     2341 cgggaattcg tctggctgcc attgcggact ttcggcaaga agcgcctcaa tgcggctcaa
     2401 gcatagtgct acgcgggcgc gctcattagc attgagcccc g
//
LOCUS       DAOQNZ010000568         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25671_length_2437_cov_3.251889, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000568 DAOQNZ010000000
VERSION     DAOQNZ010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25671_length_2437_cov_3.251889"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKW73_10575"
     CDS             <1..181
                     /locus_tag="PKW73_10575"
                     /inference="COORDINATES: protein motif:HMM:NF018531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNA73776.1"
                     /translation="GKEGLSEPLSTVSEEGLTVAAYRAQGEKCARCWKYTGDVGKDSQ
                     YVDLCGFCADVVSRL"
     gene            complement(211..996)
                     /locus_tag="PKW73_10580"
     CDS             complement(211..996)
                     /locus_tag="PKW73_10580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA73777.1"
                     /translation="MYKSKLLTFFSLLVYLFLYAPILVLIIFSFDESRLGVKWTGFTW
                     KWYQSLFLDENVLSALSNSLIVAAAAVTISTVLGVLAAVGLAGIKNKKLEAAARGLLI
                     LPVLVPEIAMAVSALLLFISLGFSLGLTSVILAHVVFCLSYVTMTVSARLEGMNPHLR
                     EAALDLGVSPVKAFFKVTLPLLTPGIISGAMLAFVLSLDDFVITQFTAGVGATTLPLR
                     IYSMVKFGVSPEINALSTLMLVVTLLIAGSAEFIRFRSEKPAH"
     gene            complement(1012..>2437)
                     /locus_tag="PKW73_10585"
     CDS             complement(1012..>2437)
                     /locus_tag="PKW73_10585"
                     /inference="COORDINATES: protein motif:HMM:NF020253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73778.1"
                     /translation="LAPEAQKQAIIEHLERFIFADKVEVVPLVFPFCQLVSGSHASLK
                     LPSPASTRAAFMPLNKGFIARIHITPCPTFIIFSEEDSLTLPQLSREELLIRRVEAGQ
                     AEFLTDYSDSLIVELSLSSPAVSYTKGCFQGQEVLARVKAQGSPSRQLVGLVLKSSEA
                     INFPVGSQFHLNAEETAGTLASHGFSPRLSKHIALAFLKRDLRVPGREIEGYVEVEGK
                     RLPLKAQVQVLPFMEPEKDEDLAQSLYEEALQDFTAGNGAVAIQTLKQVVLLAPSFED
                     GYEALGVMLSKVDDGKHIDEAIACMKELAALNPDSIMAHANLSVFYLEKGDKEMAEEE
                     KAVSMSIRMRQAAKEAMQEKNAREEQEKERKEAAERIKLFQEVLEIDPDDLFANQGLG
                     NCYNILGEHERAIGYLEKAIAIKENHTQCYEDLGKAYEALGNLARAAEYYGKGVEIAA
                     KKGDMQPLKAMKAKLEALLLQSGK"
BASE COUNT          553 a          618 c          699 g          567 t
ORIGIN      
        1 cggcaaggag gggctttccg aaccgctctc cactgtttcg gaagaaggtc tgaccgtggc
       61 tgcttaccgg gcccagggtg agaagtgcgc acgctgctgg aagtataccg gcgatgtcgg
      121 taaagacagc cagtacgtgg acctctgtgg tttctgcgcc gatgtggtaa gccggctcta
      181 aagctcacca tcaggctacc tcaagtcagc ctaatgggct ggtttctccg agcggaagcg
      241 aataaactca gcgctgcctg caatcagcaa ggtgaccacg agcatcaaag tggaaagggc
      301 gtttatctct ggagagacgc cgaacttgac catgctgtag atgcgcagtg gtagggtggt
      361 agctcctact ccggctgtaa actgcgtgat tacaaagtcg tctaaggaaa gcacgaaggc
      421 cagcatggca ccggatatga taccgggggt gagaagcggt agcgtcactt tgaaaaaagc
      481 cttcaccgga ctgacaccca ggtcgagagc ggcttctctt agatgtggat tcatgccttc
      541 caggcgagcg gagacggtca tggttacata ggaaaggcag aagaccacat gggccagaat
      601 gacggaagta agaccaaggg agaagcccag gctgatgaag agaagcaggg cggagacggc
      661 catggctatt tccggtacca aaaccggtaa gataagcagt ccgcgtgcgg ctgcttccag
      721 cttcttattc ttgataccgg ccagtcccac ggcggcaagc acgcccagca ccgttgaaat
      781 tgtcacggcg gcggcggcta cgatcaaact attggacagg gcagagagta cgttttcatc
      841 cagaaagaga gactggtacc atttccaggt gaagccggtc catttgacgc ccaggcggct
      901 ttcgtcaaag ctgaagatga ttagaactag aattggcgca tagagaaaga gatacacaag
      961 taagctgaag aaggtcaaaa gcttgctttt gtacatgtgt agtctgcctc tctacttgcc
     1021 gctctggagc aaaagggctt ccaatttggc cttcatagct ttcagtggtt gcatgtcgcc
     1081 tttcttggcg gcaatctcca cacccttgcc gtaatattcc gcggccctgg ctaaattgcc
     1141 cagggcttca taggctttac ccaaatcctc atagcactgg gtgtgatttt cctttatggc
     1201 aatggccttc tccagataac caatagcccg ctcatgctcg cctaagatgt tgtagcaatt
     1261 gcccagtcct tgattggcga agagatcatc gggatcgatt tccagtactt cctggaagag
     1321 cttgatgcgc tcggcggctt ctttgcgttc cttttcctgt tcttctctgg catttttctc
     1381 ctgcatggct tccttagcgg cctggcgcat gcggatgctc attgaaacgg ctttttcttc
     1441 ctccgccatt tccttgtcgc ccttttccag atagaaaacc gagaggttgg cgtgggccat
     1501 gatcgaatcg ggattgaggg cggcaagttc tttcatgcag gcgatggcct cgtcaatgtg
     1561 cttaccatcg tccactttgg aaagcattac cccaagagcc tcgtagccgt cttcgaaaga
     1621 gggcgccagg aggacgactt gtttgagggt ctggatggcg acggcgccgt tgccggcggt
     1681 aaagtcttgc agtgcttctt catagaggct ctgagcaaga tcctcgtctt tctccggctc
     1741 catgaagggc aagacctgca cctgcgcttt gagcggcagg cgcttgcctt caacttccac
     1801 atagccttca atttcgcggc ccggcacgcg caaatcgcgc ttgagaaaag cgagggcaat
     1861 atgtttggac agtctcgggc tgaagccatg ggaagccagg gttccggctg tttcttccgc
     1921 atttagatgg aactgcgagc cgacgggaaa gtttattgct tcgctgcttt tgagcaccag
     1981 acccaccagt tgtctggaag gcgagccctg ggctttcact cttgccagaa cttcctgacc
     2041 ctgaaaacaa cctttggtgt aagagacggc cggtgaactc aaggatagtt ccacaatcaa
     2101 gctgtcgctg tagtcggtga gaaactccgc ttgcccggcc tcgacgcgac gaataagcag
     2161 ttcttccctg gaaagctggg gtagagtcag tgaatcttct tcgctgaaga taatgaaagt
     2221 aggacagggc gtaatatgga tcctggcgat gaagcccttg ttgaggggca tgaaggcggc
     2281 tctggtgctt gccggtgaag gaagcttgag ggaggcatgg ctgccgctca caagttggca
     2341 aaagggaaat accaggggca cgacttccac tttgtcggca aatataaagc gctccaggtg
     2401 ctcgatgatg gcttgtttct gcgcctcggg ggcgaga
//
LOCUS       DAOQNZ010000569         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25716_length_2435_cov_4.294958, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000569 DAOQNZ010000000
VERSION     DAOQNZ010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25716_length_2435_cov_4.294958"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PKW73_10590"
     CDS             <1..1056
                     /locus_tag="PKW73_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73779.1"
                     /translation="WRMEGYSREHLSSAGLRDVEHSLSKPPGTYRIAIMGDSAAEGLQ
                     VALNETYARRLEQLLNEDLQAGGSLAKKLFMQEVQHNFAPNAAAGTSPQAGAIKHFEV
                     INFGCSSYSTGQELLQFKKEVAQYKPDLTIVMICKGDTLENNIDVTQRGKAEPKPYFY
                     LDQTGKLELDTSILQENKDKLSLDSPTNKVLNWLRANSNLYGAVNRTNFNLFLSDKAY
                     FRLTRFAQDMGKRLATQKEAIAVPLYGVQNNRQVTLAVIKEFQKTARAAGSQFAMTLF
                     PDVTASDKDWADTITAIKAQASSDNFGVIDLQPPFRKDKNPSGLFLQFHFSKEGHELT
                     ARTIKEFLENTPAKPTP"
     gene            1193..1651
                     /locus_tag="PKW73_10595"
     CDS             1193..1651
                     /locus_tag="PKW73_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73780.1"
                     /translation="MSEPPSEQELRDQIIHYTCLKNCYGKKGNAGACCTIQDRDWIIG
                     PITDPKDFLKRLRSHFGREFRYDEVFIDYKEGHKLFPDKATWQNPKHYPALRVLLDPE
                     AVYPCPFLGEQRQCTIQEIKPKICSDYLCDHLKSVISQTLGDDKKAPSAK"
     gene            complement(1725..2246)
                     /locus_tag="PKW73_10600"
     CDS             complement(1725..2246)
                     /locus_tag="PKW73_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73781.1"
                     /translation="MSVTDNIPKNGDRAHESQNVDWAGNDTRVNLMSDVMKTVESGKM
                     SNAMSDVGKGGKDNSLVGSEVEKTIVGADNDVQKAFGGKGDSITLDEASKAEREKAMD
                     GKSFDAANLYNKKDSANMAVDPERDKYAAAYYAANVMGDASRQAELSKAFDNAFTNNP
                     HLQQNQQREYAHA"
BASE COUNT          664 a          689 c          578 g          504 t
ORIGIN      
        1 tggcgcatgg aaggctacag ccgcgaacac ctcagttcag caggcttgcg tgatgtggaa
       61 cacagcctga gcaaaccacc aggcacctac cgcatcgcca tcatgggcga ttccgccgcc
      121 gagggcctgc aggtagcctt gaacgaaacc tatgcccgcc gtctggagca gctcctcaat
      181 gaagacctgc aagccggggg ctctcttgca aagaaactgt tcatgcaaga agttcagcat
      241 aatttcgcac caaatgcagc cgctggcacc tcgccccaag caggcgccat caagcacttt
      301 gaagtcataa actttggctg ctccagttat tcaaccgggc aggagctttt gcaattcaag
      361 aaggaagtgg cgcagtacaa gccggatctg accattgtga tgatctgcaa gggcgacacc
      421 ctggaaaaca atatcgatgt cactcagcgc ggcaaggcag aacccaagcc atatttctac
      481 ctggatcaaa caggcaaact ggaactcgat accagcatct tgcaggaaaa caaagacaaa
      541 ctcagcctgg atagccccac caacaaagta ctcaactggt tgagagccaa tagcaacctc
      601 tatggcgccg tcaatcgcac caatttcaac ctcttcctct ccgataaggc ctatttccgc
      661 ctcacccgtt ttgcacaaga catgggcaag cgcctggcta cacagaaaga agcaatagca
      721 gtgccgcttt acggcgtcca gaacaaccgc caggtgaccc tggccgtgat caaagagttt
      781 cagaagactg cccgggccgc cggcagccag tttgccatga cactctttcc cgacgtcacc
      841 gcctccgaca aagactgggc cgacaccatc accgccatta aagcccaggc aagctctgac
      901 aacttcggcg tgatagactt gcaaccgcct tttagaaaag acaagaaccc atcaggttta
      961 ttcctgcagt ttcacttttc caaagagggg catgaattaa cggcaaggac aataaaggaa
     1021 tttctggaaa acactccggc aaaaccgaca ccttaagtca gcagatgact gcatcaaatg
     1081 cagagagtgg tttccgcgaa ctatctcaat cgaagccaat tcaaaccgtc ctaaccagag
     1141 cctaaaccga cgcaacagcg aacgccgata gaaagcagaa ggccctcaaa caatgagcga
     1201 accacccagc gagcaagaac tgcgcgacca gatcattcac tacacctgcc tgaagaactg
     1261 ctacggcaag aaaggtaacg ccggcgcttg ctgcaccatt caagaccgag actggatcat
     1321 cggaccgata accgacccga aagactttct caagcgcctc cgcagccact ttggtcgaga
     1381 attccgttac gacgaagtct tcatcgatta caaagaaggg cacaagctct ttcccgataa
     1441 agccacctgg caaaatccca agcactatcc ggctttgaga gtacttttgg acccggaagc
     1501 cgtttacccc tgcccatttc tcggggaaca gcgccagtgt accattcagg aaatcaagcc
     1561 gaagatctgc tcagactacc tgtgcgatca cctcaagtcg gttattagcc agaccctggg
     1621 cgacgacaag aaagcaccct cagccaagta agatgatccg ataaaatagc ccaagccgaa
     1681 acaccaaccg gttaaggtag aaaaagcaag cggcaaggca ccgatcaggc gtgagcgtat
     1741 tcgcgctgct gattttgctg caagtgcggg ttattggtaa atgcgttatc aaaagctttg
     1801 gaaagctcgg cctggcggct ggcgtcaccc atgacattag cggcatagta agcagcggcg
     1861 tacttgtctc tttcagggtc tacagccata ttggcggagt cttttttgtt gtagagattg
     1921 gcggcatcaa aggatttgcc gtccatggct ttttctctct cagctttgga ggcttcatcg
     1981 agggtgattg aatcgccctt gccgccgaag gctttttgaa catcgttatc ggcgccgacg
     2041 atggtttttt caacttcgct gccgaccaga gaattatctt tgccgccctt gcccacatca
     2101 ctcatggcat tggacatttt gcctgactca accgtcttca tgacgtcgct catgagattg
     2161 acgcgggtgt cgttacctgc ccaatcgacg ttctgggatt catgagccct gtcgccgttt
     2221 ttcggaatgt tgtctgttac tgacatggga gagtcctggt aaatacgacc tggataaaga
     2281 tacctcacta ttagatgtga tccgccggtc attgtcaagc agattttatc taagccagcc
     2341 cactcgacaa aggctcaccg gtgccgcaat acaggtggaa agcgcggaaa accaagcaaa
     2401 aaattgagcc agacatctat atacagaagc cccgc
//
LOCUS       DAOQNZ010000570         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25746_length_2434_cov_3.626734, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000570 DAOQNZ010000000
VERSION     DAOQNZ010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25746_length_2434_cov_3.626734"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKW73_10605"
     CDS             <1..375
                     /locus_tag="PKW73_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73782.1"
                     /translation="PSKRSLIKKKEGMLAHIFIGPSFFLCAVLCLCAGADMLGWPGTK
                     TLNLVLNGGDTNKPTAVSFDENTGRYTFPILAKSGGKLYKQLFESKISEDKDIIGRKL
                     DKMIPDQERNIDGAMVKNANGK"
     gene            564..737
                     /gene="rpsU"
                     /locus_tag="PKW73_10610"
     CDS             564..737
                     /gene="rpsU"
                     /locus_tag="PKW73_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNA73783.1"
                     /translation="MSEVRVAEGESIEAALKRFKKKIQKAGILSEIKRRERYEKPSVK
                     RKRKAEAARKRRS"
     gene            1128..1391
                     /locus_tag="PKW73_10615"
     CDS             1128..1391
                     /locus_tag="PKW73_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73784.1"
                     /translation="MTNSNKGKANEKSQAMVSVETGNLKERLNKLYDCRRGASKQLVQ
                     EGLFEYLWDLKEKALLEKRTTVEVPGNWLDELEELQDDRRAGS"
     gene            1436..2281
                     /locus_tag="PKW73_10620"
     CDS             1436..2281
                     /locus_tag="PKW73_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73785.1"
                     /translation="MSSVVFCGTVLTCGAAFFCSSPALAQGNAANSNPSVSNAASALS
                     KAQARARGSRLLPGKARLPLYRSGKTQKQMLAKEILLASLAVRRAEAKLDSQLEALAG
                     KLPQLLHKQELTGSPSDDLPWRRRLADQVGANPFDLEPELSGEMARARATWSANHSGG
                     MPRNNREPAQPEDTGKEPSKDPLKGPLHEPVTEQRSHETRESEPRVYLVRVPAPSLKA
                     IVEERQLPSLTRQQIEQTDNTPGTIYVLLAEDQTYLLIALTIDGNPLCASNPQEGLRG
                     VLGHL"
BASE COUNT          650 a          666 c          645 g          473 t
ORIGIN      
        1 ccgagcaaac gctcactaat taagaagaaa gagggcatgc tggctcacat ctttatcgga
       61 ccttccttct ttctctgcgc cgtgctctgt ctatgcgccg gtgccgatat gctgggctgg
      121 ccgggtacca aaactctcaa cctggtcttg aacggtggcg acaccaacaa gccaacagcg
      181 gtttccttcg acgaaaacac cggtcgttat acctttccaa tcctggctaa gtcaggcgga
      241 aaactttaca aacagctctt tgaatccaag atttcggaag acaaagacat catcggtcgc
      301 aaactcgaca agatgattcc cgaccaggaa cggaacatag acggcgccat ggttaaaaac
      361 gccaacggta agtaagccgc agcacaaatt tcaagaaaag gaagggtcat caagtgcacc
      421 cttccttttc cttaggctct atttagttaa gtagtgtcta tggaatgctc ttttttggcg
      481 ctagatgagc ccggaggtga taacatctat ggtttgcata tatacagtgc accagactta
      541 attctagatt ggggggagga cctttgtcag aggttagagt agccgaaggc gaaagcatcg
      601 aagcagcgct caagcgcttc aaaaagaaaa tacaaaaggc cggaattctt tctgaaatca
      661 aacgcagaga gcgttacgag aagcctagcg taaagagaaa gcgcaaagcc gaagctgcca
      721 gaaagcgtcg ctcctaattt agagcaaaga gttagtcgcc gccgcctgta aggcgccgtg
      781 cgcactcggg cctcatgcct tccagagtaa gctctgggca agcatgaggc ttttgcatgt
      841 ccaaagaagc ttcttgcttg aaaaaccagt taaaggtcca attctgtggc gtttccgtct
      901 accgcagcgg ggctttagag cctctcctta aaatgtggga gaagtaaaaa atcgccaggg
      961 ttatcaaggg attattcggg gtacgagccg ggcataaaag atgcacaggc aaatttcgca
     1021 ctagattcct gccgttggct tgatagaaca tcagaccgca ccagttgcac ttgagcaaag
     1081 gtgagggttt tccacgcggc aagcaaccaa accggagaat gaatacaatg actaactcaa
     1141 acaaaggcaa agcaaatgag aagtctcagg caatggtctc ggttgaaacc ggcaatttga
     1201 aagagcgttt gaataaacta tacgactgcc gccggggagc cagcaaacag ttggtacagg
     1261 aaggtctctt cgagtatctt tgggacttga aggagaaagc tctcctggaa aaacggacca
     1321 ccgtggaggt gcccggcaat tggttggacg aactggaaga actgcaagac gaccgtcgcg
     1381 ccggctctta agcgcagcca ggccggctgc ctgcctcaag tcagctacag attatttgag
     1441 ctccgtagtc ttttgcggca ccgtactcac ttgcggtgcc gccttctttt gcagcagccc
     1501 ggctctggcc cagggcaatg ccgccaattc caacccttcc gttagcaatg ccgcctccgc
     1561 tctcagcaag gcccaggcga gagcccgggg cagccgcctc ttgccgggta aagcgcgact
     1621 gcccctttac cgctccggca agactcagaa acagatgctg gcgaaagaaa tactgctggc
     1681 cagcctcgcg gtcaggcgag cagaagcaaa actggacagc caacttgagg ctctggccgg
     1741 gaaactgccg cagcttctgc acaaacagga attgaccggc tccccctcag acgacctgcc
     1801 ctggcgcagg cggctggcag accaggtggg cgccaacccc ttcgatctgg agccagagct
     1861 gagcggcgaa atggccagag ccagggccac ctggagcgcc aatcacagcg gcggtatgcc
     1921 cagaaacaac cgggaaccgg cacaaccgga agatacaggc aaggagcctt caaaggatcc
     1981 cttaaaaggt ccgctccacg agccggtgac tgagcagcgc tcccacgaaa ccagggagag
     2041 cgagccccgg gtctatctag tgcgggtgcc ggccccgtcc ctcaaggcaa ttgtggaaga
     2101 gcggcaactg ccttccctga ccagacagca aatagaacaa accgacaaca cgccgggcac
     2161 tatatatgta ctgctggctg aagaccagac ttacttgctg attgccctga caatagacgg
     2221 caatccactt tgcgcgtcca atccgcaaga aggcctgcgc ggcgtgctcg ggcacctcta
     2281 aagcctgtcc ccatgagaat tgagcacaga ttcgccaaaa agctacctgg ttcaagtcag
     2341 ggcctaccac cagaaaagat ctaggctaat tcccaggttc ccggcggctg cttttagcta
     2401 accttaaatg tagcgctggc cccaagggtt ctac
//
LOCUS       DAOQNZ010000571         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25789_length_2432_cov_3.934792, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000571 DAOQNZ010000000
VERSION     DAOQNZ010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25789_length_2432_cov_3.934792"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PKW73_10625"
     CDS             complement(<1..934)
                     /locus_tag="PKW73_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNA73786.1"
                     /translation="MKTNHNLTQLWKAGSCHAARLAYRLGLSDKYCFPPNFVWGTGTS
                     AYQVEGAHDQDGKGESIWDRFCRSPGNIKNGDTGNIACDQYHRFAQDMELCRRLNLGA
                     YRFSIAWPRVQPLGRGALNEKGMSYYDRLIDELLALGIEPVITLYHWDLPQALQDDLG
                     GFAHRDTVQHFADYTEAMVHRYSDRVKFWTTLNEPWCSSFLGHQTGYHAPGIKDPLIA
                     AQVAHNLLLCHGAGTIAARAAARQKLDLGIVLNLTISEALNPADAHLADKAFARDCGF
                     YLEPLLRGKYSQAALAGIKDFKEQDLRLINQPMDY"
     gene            complement(971..1528)
                     /locus_tag="PKW73_10630"
     CDS             complement(971..1528)
                     /locus_tag="PKW73_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73787.1"
                     /translation="MTGSEMSDLVRPVALAICTALGTLFLVLGLVTTSPISTIAYLLA
                     GVLLLLAFYLFLLGHYVTHRRKVDDAKERQEFAARNPALHKALVKLEAICDGLPGYKR
                     IRFHRSAEEGVNHGEAQLNVYVYVDGWSAANNTGEPVSVGGMPIQDWFNKVPKELDGY
                     EVEVTIFHNSISMGFVGGLGNGFWT"
     gene            complement(1626..1952)
                     /locus_tag="PKW73_10635"
     CDS             complement(1626..1952)
                     /locus_tag="PKW73_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73788.1"
                     /translation="MQFTQITFAAMAIIGAALGYFLFQREKKYSHPGVGTVLAALWIV
                     QVVVVTIGLSLVQWLGQESIKPGDLHGLPTAFWVVYTVWTGQALTYITLAKVYVMKNR
                     DPFSPY"
     gene            complement(2047..>2432)
                     /locus_tag="PKW73_10640"
     CDS             complement(2047..>2432)
                     /locus_tag="PKW73_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA73789.1"
                     /translation="REGPSYYAPTVLLNPPANAKVSTLEVFGPVVCVYSYKELDEAIA
                     AANSLPCAFQAAVFSKDLDTAMRVTRHLNATAVMVNDHTAFRVDWMPFGGRKESGLGL
                     GGIAHSVKEMTQDKLMVIKSSGMDS"
BASE COUNT          485 a          598 c          773 g          576 t
ORIGIN      
        1 ggtagtccat aggctggttg attagcctca gatcctgctc cttgaagtct ttgatgccgg
       61 ctagggcggc ctgggaatat ttgcctcgca gcaggggctc cagataaaag ccgcagtctc
      121 tggcgaaggc tttgtccgcc aggtgtgcgt cggccgggtt gagggcttcg ctgatggtca
      181 ggttgagaac aatacccagg tcgagctttt gtctggcggc ggcgcgggcg gcgattgtgc
      241 ctgcgccgtg acagagcaat aagttgtggg ctacctgggc ggcgatgagc ggatctttga
      301 tgccgggcgc gtgatagcct gtttgatgtc ccaggaagga agagcaccag ggctcgttca
      361 gggtggtcca gaacttgacg cggtcgctgt aacgatggac catggcttcg gtgtagtcgg
      421 cgaaatgttg gacggtgtct cggtgcgcga aaccgcccag atcatcttgc agagcctggg
      481 gtaggtccca gtggtaaagg gtgattaccg gttcgatgcc cagtgcaaga agctcatcga
      541 tgaggcgatc ataatagctc atgccttttt cgttgagggc gccgcgaccg aggggttgca
      601 ctcggggcca ggcgatggaa aagcggtaag cgcctaaatt gaggcggcgg cagagttcca
      661 tgtcttgagc aaaacggtga tactggtcgc aggctatgtt gccggtgtca ccgtttttga
      721 tgttgcccgg gcttctacag aaacggtccc agattgattc tcctttgccg tcttgatcgt
      781 gggcgccttc cacctggtaa gccgaggtgc cggtgcccca gacgaaattg ggtgggaagc
      841 agtacttatc actgagaccg agtctgtagg cgaggcgggc tgcatggcag ctgcctgcct
      901 tccacagttg tgtcaggttg tggttggttt tcatagaggc agggtttcct tgaactattt
      961 gctgttgcag ctaggtccag aagccgttgc ccagtccgcc cacaaagccc atagagatgc
     1021 tgttgtggaa gatggtgact tccacttcgt agccgtccag ctctttgggt actttgttga
     1081 accagtcctg gatgggcatg ccgcctacgg aaacgggctc gccagtgtta ttggctgcgc
     1141 tccagccgtc tacgtagaca tagacattta gctgggcctc gccgtggttg acgccttctt
     1201 cggcggagcg atggaagcga atgcgtttgt agccgggcaa accgtcgcag atggcttcca
     1261 gtttgaccag cgccttatgg agagcaggat tgcgcgcggc aaactcctgt ctttctttag
     1321 cgtcatcgac cttgcgccgg tgcgttacgt aatgacctag caggaacagg taaaatgcca
     1381 gcagcaggag cacgccggcc agcaagtagg ctatggtgct gatggggcta gtcgtaacca
     1441 gacccaagac taagaatagg gtgcccagcg cggtgcagat tgcaagtgcg acggggcgga
     1501 ctaaatcgct catttctgaa ccagtcataa ttgagatctc caattgtttg ttaggggtgt
     1561 gttgaggaaa ggaggcggtc cggaaacccg gagccgccct gtgttttttt aagttggcga
     1621 agccttcagt aggggctgaa ggggtcgcga tttttcatga catacacttt ggctaaggta
     1681 atgtaagtga gagcctgacc ggtccatacc gtatagacga cccagaaggc ggtcggtaga
     1741 ccatgcaggt cgccaggctt gatggactct tgcccaagcc actgtaccag gctcagaccg
     1801 atggtcacta ccacaacctg gacgatccag agggcagcca gtacagttcc tacgcccggg
     1861 tggctgtatt tcttttctct ttgaaagagg aagtagccca gagcggcgcc gataatagcc
     1921 attgctgcga aggtgatttg ggtaaattgc ataggatttc ctgtcagtaa gtgatttgaa
     1981 tttgaaagaa aaccggagcc acacttagtg cgactccggt ctttgctctg ctgtggcgtg
     2041 gtagtgttag ctgtccatgc cgctgctttt aatgaccatc agtttgtcct gggtcatttc
     2101 cttgacactg tgggcgatac cgccgagtcc cagtcccgac tctttgcgtc cgccgaaggg
     2161 catccagtcg acgcggaagg ctgtatggtc attgaccatg acggcggtgg cgttcaggtg
     2221 cctggtgacg cgcatggccg tgtccaggtc tttactgaag acagcagcct gaaaggcgca
     2281 gggcaggctg ttggctgctg caatggcttc gtccagctct ttgtaggagt agacgcagac
     2341 cacgggaccg aagacttcca gggtagagac cttggcattg gccggtgggt tcagtagcac
     2401 ggtgggagcg tagtagctgg gtccttccct cc
//
LOCUS       DAOQNZ010000572         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25796_length_2432_cov_3.285233, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000572 DAOQNZ010000000
VERSION     DAOQNZ010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25796_length_2432_cov_3.285233"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1388
                     /locus_tag="PKW73_10645"
     CDS             303..1388
                     /locus_tag="PKW73_10645"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNA73790.1"
                     /translation="MKIVIFGGGLQGLVIANNLRAREEKPEIVIADIVRPAGLAEDSP
                     IKWVKADVLAGEQVKAVVSGASACVLAVPSSIAHQALANIIACGVPVADVSFTPDPPL
                     SLSEAARKSGSSCIVDCGVAPGLSHILVGHAYKQLAGLDKVTILVGGMPQKPPAVFHH
                     AVYFNPCDLLAEYVRPARARTKGKDIAPHPLEAPIEPYHDSDLGAIESFLSDGLRSLV
                     TSFPDVPCMEERTLRWQGHLRTMKDLHELGFFLEGMLPNTAQSINNKYPGKDFPDYLL
                     MVVEAEKGSRKLSWRLLDQETDGVSAMSRTTGFTTAAVAMVLARGQFKEPGVHPPEVL
                     GQDAAMVELILADLAERGVKTKELVNK"
     gene            1475..>2432
                     /locus_tag="PKW73_10650"
     CDS             1475..>2432
                     /locus_tag="PKW73_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73791.1"
                     /translation="MSKPESQSLNAAPEQASRAVDDSQQRNSQDSMTDWMKNFAGQNS
                     PQIMESTALTSAMVQAKILPSMEIAGMRKDMNFQPGFMMAGMGNDRSPLPSLKIEGMA
                     DTRSSGNGSPAVEGQGRENSQIDKSRTKLLDPQASPQERLSAARELVKQNITQIDTTD
                     ATGKKSSLRLESEAVGGKNKEMVHVFVKDGSQKEQVALRGNFSGDLLEKQIRDGKAVS
                     HEGRGLTRLNQLQTGKQTKAEAEKPVESKQPTEVRTQAEVKAPVESKRPAEDKRPAEV
                     RTPSEVKPPVEVKPPVETKTPAEKEPQQTSTTDKPPQVKTKQG"
BASE COUNT          652 a          698 c          645 g          437 t
ORIGIN      
        1 cagggcgggc gttcccacaa ctatttcttt tacttgcctg cacatgactg gcgtcccgca
       61 acttgagcga cactacggct tccacaattc cagtctgaca ccgggcaaag ggaacgccca
      121 tccacgcttt tacctatttt caagggcaat ttcagaaccg ccctcggtca tttgaccgat
      181 gcccgggtct aaccggagga gagaagctat agcgatgctt aaggaagctg cgcggggctt
      241 ggtctaaaat caggttttca agtaactgtg tttgcttgca agcacgataa ggggcaggga
      301 aattgaaaat cgtaatattc ggcggcggac tgcaagggct ggtcattgcc aacaatttaa
      361 gagccagaga agaaaaaccg gagattgtca tcgccgacat agtcagaccg gccggacttg
      421 ccgaggatag ccccataaaa tgggtgaagg ccgatgtact ggcaggcgag caagtcaaag
      481 ctgtagtcag tggcgccagt gcctgcgtgc tggctgtacc gagcagtatt gcccaccagg
      541 ctctcgctaa catcatcgcc tgcggagtgc ctgtagccga cgtcagcttc actcccgatc
      601 cgcccctcag cctctcggaa gccgcccgca aaagcggctc cagctgcatt gtggattgcg
      661 gcgtcgcccc ggggctgagc cacattctgg tgggacacgc ctataaacaa ttggccggtc
      721 tcgataaagt caccatcctc gtgggtggca tgccccagaa gcccccggcc gtcttccatc
      781 acgctgttta tttcaacccc tgcgatctcc tggccgaata cgtgcgcccg gctagagcca
      841 gaacaaaagg caaggacatt gccccccacc ccctggaagc cccaattgag ccctatcatg
      901 atagcgacct gggtgccatc gagtccttcc tctcggatgg actgcgctct cttgtcacaa
      961 gctttcccga cgtaccctgc atggaagagc gaaccttgcg ctggcagggt cacctgagaa
     1021 ccatgaaaga cctgcatgaa ctgggctttt tcctggaggg aatgctgccc aacacagccc
     1081 agagcataaa caacaaatac cctggcaagg actttcccga ttatctgctc atggtcgtgg
     1141 aagccgagaa aggctcccgt aaactgtcct ggcgcctctt agatcaagaa accgacggtg
     1201 tttcggccat gagccgcacc accggtttca ccactgctgc agtagccatg gtcttagccc
     1261 gcggtcagtt caaagaacca ggtgtgcacc cgccggaagt gctcggtcag gacgccgcca
     1321 tggtcgaact gatactggcc gaccttgccg agcgtggcgt caaaaccaag gagctggtaa
     1381 acaaataggt tgaaatagct ctttcacagc tatttcacgc tcagccccct aatctcccag
     1441 gtgttagcac cttttctata ccgggagcgc gagcatgagc aagcctgaat cccaatcttt
     1501 aaacgccgct ccggaacaag cctctagagc tgtggacgac agccaacaga gaaacagcca
     1561 ggacagtatg actgactgga tgaagaactt tgccggtcag aacagccctc agattatgga
     1621 aagtacggcc ctcacctcag ccatggtaca ggcaaaaatc ctgccttcca tggaaatcgc
     1681 cggcatgcgt aaagacatga acttccagcc gggcttcatg atggccggca tgggcaacga
     1741 cagatcgcca ttgccctctc tcaagataga aggcatggcc gataccagaa gtagcggcaa
     1801 cggaagccct gccgttgaag ggcaggggag agagaacagc cagatcgata aatccagaac
     1861 taaactgctt gatccgcagg ccagccccca ggagcggctc tctgccgcta gagaactggt
     1921 caagcaaaac atcacccaga ttgataccac cgatgccacc ggcaagaaaa gcagtctgcg
     1981 actggagtct gaagcagtag gcggcaagaa taaagagatg gtgcatgtct ttgtcaaaga
     2041 cggttcccag aaagaacagg tggcactgcg cggcaacttc tcaggcgatc tactggagaa
     2101 gcaaatcaga gacggcaaag ccgtcagcca cgaagggcgc ggtctgactc gcttgaacca
     2161 actgcaaacc ggcaagcaga cgaaagcgga agcggaaaaa ccggtcgaaa gcaagcaacc
     2221 cactgaagtt agaacacagg ctgaagttaa ggcaccggta gaatcaaaga gaccggctga
     2281 agataagaga cctgctgaag tcagaacccc gtccgaagta aagccgccgg tcgaagtaaa
     2341 gccgccggtc gaaaccaaga ccccggcaga gaaagagccg caacaaacaa gtacaaccga
     2401 taagcccccg caagtaaaaa ccaaacaagg ca
//
LOCUS       DAOQNZ010000573         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25813_length_2431_cov_3.222643, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000573 DAOQNZ010000000
VERSION     DAOQNZ010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25813_length_2431_cov_3.222643"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PKW73_10655"
     CDS             complement(<1..250)
                     /locus_tag="PKW73_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73792.1"
                     /translation="MKKLLNVSLALILLAGPGWQQGAPARASEKKSGSETHYAPCMSW
                     VSKTGRAEAVFLCIHGLGLDATTYNTFAQSMTEKGITCY"
     gene            complement(254..772)
                     /locus_tag="PKW73_10660"
     CDS             complement(254..772)
                     /locus_tag="PKW73_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNA73793.1"
                     /translation="MKTFAMLVVIMGVSGSGKTTIGKALAKRLGIDFADGDDYHSQAN
                     KEKMAAAIALSDADRLPWLQELNKLLLSYQRDDRGLVLACSCLKKNYRKQLSSGLEAV
                     RFVYLKLSQEEATKRLAGREDHFFNPALLRSQFETLQEPGEEEALIIESRGDSAHCLE
                     GIIDRLEKLVPR"
     gene            842..>2431
                     /gene="glgB"
                     /locus_tag="PKW73_10665"
     CDS             842..>2431
                     /gene="glgB"
                     /locus_tag="PKW73_10665"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNA73794.1"
                     /translation="MTTSTRSFFSDFDAHLFSEGSHYRIYQKLGAHQIVMDGKKGTHF
                     AVWAPNAESVAVIGDFNDWNAAKCPMQMQGSTGVWTCFVPGVKEGALYKYYVVGKHGV
                     RAEKTDPVGFFAETRPRTASCVFDLDTYQWQDQKWLEKREKTNHLEAPVSIYELHLGS
                     WMREHDPDGVERQDRFLTYRELADKLIPYVQELGFTHVEMLPLSEHPLDRSWGYQTTG
                     YFAATSRFGTPTDLMYLIDKLHQAGIGVIMDWVPAHFPRDGHALGLFDGTHLYEHADP
                     RQGEHREWGTYVFNYGRYEVSNFLLSNAMFWFDKYHIDGLRTDAVASMLYLDYAREGG
                     DWLPNQYGGRENVEAIDFLRRLNEKVYLEYPSVMMIAEESTAWPQVTRPTYLGGLGFG
                     LKWDMGWMNDTLKYFQLDPVYRKFHHDKLTFRAIYSSSENFILPLSHDEVVHYKRSLL
                     SKMPGDMWQQFANLRLLLGYQYTTPGKKLLFMGGEFGQWIEWNEDQSIDWHLTQYFSH
                     QGMQRFTADLNKLYRDEPALYE"
BASE COUNT          525 a          670 c          618 g          618 t
ORIGIN      
        1 cataacaggt gatgcctttc tcggtcatgg actgggcgaa ggtattataa gtggtggcat
       61 caagaccgag tccgtggatg cagagaaaga ccgcttccgc ccgtccggtt ttgcttaccc
      121 agctcatgca tggcgcgtaa tgtgtttcgc tgccgctctt tttctcggag gcgcgggccg
      181 gtgctccctg ttgccagcca gggccggcta agagtattag tgcaaggctt acattcagga
      241 gtttcttcat ggtttagcgc ggtaccaatt tttccagtct gtcgattatg ccttccaggc
      301 aatgggccga gtccccacgg ctctcaatga tcaaggcttc ttcttccccc ggttcctgta
      361 aagtttcgaa ttgactcctg agtaaggcgg gattaaagaa atggtcctca cgaccggcaa
      421 gtcgctttgt cgcttcttcc tgggacaact tgagatagac aaagcgtacc gcctcaagcc
      481 ccgagcttaa ctgttttcga taatttttct taaggcagga acaggccaga accagacccc
      541 gatcgtctct ttgatagctg agcagaagct tattgagctc ttgcagccag gggaggcggt
      601 ctgcatcgct gagtgcgatg gcggcagcca ttttctcttt gttggcctgg ctatggtaat
      661 catcaccgtc ggcgaaatct atgcccagtc tcttcgcaag agccttccct attgtggttt
      721 tcccggaacc ggaaaccccc attattacca cgagcattgc aaatgttttc ataggaagtt
      781 tattccctgg ctttctgtta atctaaatag tcaagttcca aatagatgcc agaggccgac
      841 gttgaccacc agcacccgct cgtttttttc cgattttgac gctcatcttt tctcagaggg
      901 cagccactat cgtatttacc agaaactcgg cgcccatcag attgtgatgg acggtaagaa
      961 gggcacgcat tttgccgtct gggcccccaa tgccgaatct gttgccgtta taggcgactt
     1021 caacgactgg aatgccgcca aatgccccat gcagatgcag ggctctaccg gtgtctggac
     1081 ctgctttgtg cccggtgtga aggaaggcgc cctctataag tattatgtgg tcggcaaaca
     1141 cggtgtgaga gctgaaaaga ccgacccggt cggcttcttc gccgagacca gaccgcgcac
     1201 cgcctcttgc gttttcgacc tcgataccta tcagtggcaa gaccagaaat ggcttgagaa
     1261 gcgcgagaaa accaatcacc tggaagcccc ggtctccatc tacgaactgc acctgggctc
     1321 ctggatgcgt gagcacgacc cggatggcgt cgagcgtcag gatcgtttcc tcacctacag
     1381 agaactggct gataaactaa ttccctacgt tcaagaactg ggcttcaccc atgtggagat
     1441 gttgcctctc tctgagcatc ccctggaccg ttcctggggc tatcagacca cgggctattt
     1501 tgccgccacc agtcgtttcg gcactcccac cgacctcatg tacctgatcg ataagctaca
     1561 tcaggccggc atcggtgtga ttatggactg ggtgcctgcg cattttcccc gggatggtca
     1621 cgccctcggt cttttcgacg gcacacattt gtacgagcat gccgatcccc gtcagggcga
     1681 gcaccgcgaa tggggcacct atgtcttcaa ctacggtcgc tatgaagtaa gcaacttcct
     1741 gctcagcaat gccatgttct ggttcgacaa gtaccatatt gacggcttgc gcaccgatgc
     1801 cgttgcttcc atgctctatc tcgactatgc cagggaaggc ggcgactggc tgcccaacca
     1861 gtatggcggt cgggaaaacg ttgaggccat tgatttcttg cgccgcctca atgagaaggt
     1921 ttatttagag tatccttccg tgatgatgat tgctgaggaa tcaacagcct ggccccaggt
     1981 gaccagacct acttatctgg gcggtcttgg ctttggtctc aaatgggaca tgggctggat
     2041 gaacgatacc ctcaagtatt ttcagcttga tccggtctat cgcaagttcc accacgataa
     2101 actgacattc cgcgccattt acagcagttc cgaaaacttt attctgcctc tcagtcatga
     2161 tgaagtggtg cattacaagc gctccctcct ttccaaaatg cccggtgata tgtggcagca
     2221 gtttgccaac ctgcgtcttt tgctggggta tcagtacacc acccccggta aaaagctctt
     2281 gttcatgggc ggtgaattcg gtcaatggat cgaatggaac gaggatcaga gcatcgactg
     2341 gcatttgact cagtattttt cccatcaggg catgcagcgc tttaccgctg atctcaacaa
     2401 actttaccgc gacgaaccgg ccctctatga g
//
LOCUS       DAOQNZ010000574         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25888_length_2427_cov_3.317032, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000574 DAOQNZ010000000
VERSION     DAOQNZ010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25888_length_2427_cov_3.317032"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2219
                     /locus_tag="PKW73_10670"
     CDS             <1..2219
                     /locus_tag="PKW73_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNA73795.1"
                     /translation="VVSYILEQSLGKALEQLPQEREKKLSALRKMKVLEPCVGTGNFY
                     FTALEMLMPLYQELGLSIAQAFEETLRLNLFAMEMDGRAITVLKLLCAALAKDITGKP
                     QLPALNCFLASDENLLGSLAPQLPESIADNQFDIVVANPPYLGRKLLDRNLKKLLKDL
                     YPGCHNELGHCFLVRSLDWVKPGGRVGFIMQSSILSLPSAASIRRDILKHCCNEQVVN
                     LGSRVFPLLSGEKADSAILILRKQEANDQTTISYFDLTRQEEKGLFLERLSKQEKGAP
                     LPFFKKQNDFNNHEGFTFNFKRPRTASLLYATLPRLSEKADIKQGLATSDNERFVRYS
                     WEIANEEIGKSYRYYIKGKGARRWYHPLEDVVFWQDQGHLVKEAVNSAYPYLKGKVHW
                     VVKNENYYFQPGLTFSFVNAQALAVRKMPAGCIFDVGGSAIFARDMEENLLLAYLNSG
                     LAMTFAQDLNPTINFQVGDLKKIPVPLFEGEGKDTLMDIAEQAVVISENLYHLESPYL
                     LPRPGTRLIDKESFANYRKNHHLTQEKLIELDCRNDELILKASATGLSLSEHTELLSW
                     LDNFWRPETAKIKEAAEYCLSELATALYLLMKALSGKIADLNEFIQLKEGTEQLMTTT
                     SLSGGSPLLVLDSIFFATALGVDLNTYLREKLNDKLRALYLQQPPYFILPCSKNTYLV
                     PFLTFKQVYLKQDCQNSKENLLPENWCREADEKLQKLAAQLEDKEARARQLFTLIQ"
     gene            complement(2240..>2427)
                     /locus_tag="PKW73_10675"
     CDS             complement(2240..>2427)
                     /locus_tag="PKW73_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73796.1"
                     /translation="SPAHARNEKSGHAKVVKSQGAKHAGAKHASAIKHPVAMAKPATA
                     TVKTSHVAHGTAAKVAH"
BASE COUNT          653 a          661 c          575 g          538 t
ORIGIN      
        1 cggtggtatc ttatattcta gaacagagcc tcggcaaagc cctggagcaa ctgccgcagg
       61 agagagaaaa gaagctttcg gcactgagga aaatgaaggt tctggagccc tgtgtgggca
      121 caggtaattt ttactttacc gccctcgaaa tgttgatgcc gctctaccag gaactgggtt
      181 taagcattgc acaggcattt gaggaaaccc tccgccttaa cctctttgcc atggaaatgg
      241 acggacgggc tatcaccgtg ttgaaacttc tgtgcgcggc cctggccaaa gacattaccg
      301 gaaaacccca gctaccagct ctcaattgct tcctggccag cgacgagaac ctgctcggct
      361 ccctggctcc ccaacttccg gagtccatag ccgacaacca attcgacatc gttgtggcca
      421 atccgcctta cctgggacgc aaactactgg acaggaatct caaaaaactt ctcaaagatc
      481 tctaccccgg ctgccataac gaactcggac actgcttctt agtcaggtca ctggactggg
      541 taaagccagg aggcagagtc ggctttatca tgcaaagctc aattctatct ctgcccagcg
      601 ccgcctccat tcgccgggac atcctcaaac attgctgcaa tgaacaggtg gtcaacctgg
      661 gttcccgcgt cttccctctt ttatccggag agaaggccga cagcgccatc ctcatcttgc
      721 gcaagcaaga ggctaacgac cagacgacaa taagttattt cgatctgacc cggcaggaag
      781 agaagggctt gttcttagaa cgtctctcta agcaagaaaa gggagccccg ctaccctttt
      841 tcaaaaaaca gaacgacttc aataaccacg aaggcttcac cttcaatttc aaaagaccac
      901 gaactgcctc tcttctctac gctactttgc ccagactgag cgagaaagcc gatatcaagc
      961 aaggactggc cacttccgac aatgaacgct ttgtgcgcta cagttgggaa attgcaaatg
     1021 aagaaattgg caaaagctat cgctattaca taaaaggcaa aggcgccaga cgctggtatc
     1081 atcccctgga agacgtagtc ttctggcaag accaggggca cctggtgaag gaggcggtaa
     1141 acagcgccta cccctacctg aagggcaaag tacactgggt agtaaaaaac gagaattact
     1201 acttccagcc cggactgact ttctcctttg tcaacgccca ggctctcgcc gtaaggaaaa
     1261 tgccagccgg atgcatcttt gatgttggcg gctcggcaat tttcgcccgc gacatggaag
     1321 agaatctctt gcttgcctat ctgaactcag gtctagccat gacttttgcc caggacctca
     1381 accctaccat aaacttccag gtgggcgacc tgaagaaaat cccggtgccg ctttttgagg
     1441 gcgaaggcaa ggacaccctc atggatatag ccgaacaagc cgtggtaatc agcgaaaacc
     1501 tctatcacct tgagtcacca tatctgctac cacgaccagg caccaggttg atagacaaag
     1561 agagttttgc caattaccgc aagaaccatc atctcaccca ggagaagttg attgaactcg
     1621 attgccgcaa tgacgaacta atactgaagg ccagcgccac cggtctcagt ctgagcgagc
     1681 acaccgaatt actcagttgg ctggataact tctggagacc ggaaacggcg aaaatcaaag
     1741 aagctgctga atactgcctt agtgaactgg ccacggccct ctacctgttg atgaaagcat
     1801 tatcgggaaa aattgccgat ctcaatgaat tcattcagtt aaaagaaggc accgagcagt
     1861 tgatgacaac aaccagccta tcagggggtt ccccgctact ggttcttgat agcatatttt
     1921 tcgccaccgc tcttggtgtg gacctgaaca cctacttaag agagaagctg aacgataagt
     1981 tgcgcgccct ctatttgcaa cagcctccct actttattct gccctgcagc aagaacactt
     2041 acctggtgcc ctttcttact ttcaagcaag tgtatttgaa acaagactgc caaaattcca
     2101 aggagaacct tttgcctgaa aactggtgcc gggaagcaga tgagaaactg caaaaactag
     2161 ccgctcaatt ggaagacaaa gaggcaaggg ccaggcaact atttactctc atccagtaag
     2221 cacgcctttc cactcatact tagtgggcga ctttagcggc tgtgccatgg gcaacatgag
     2281 aagtcttgac cgtggcagta gcaggcttgg ccatcgccac aggatgctta atagcggagg
     2341 cgtgcttagc gccggcatgt ttcgccccct gcgacttgac gactttagcg tgtccgcttt
     2401 tttcattgcg ggcatgagcc ggactat
//
LOCUS       DAOQNZ010000575         2425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25934_length_2425_cov_2.840084, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000575 DAOQNZ010000000
VERSION     DAOQNZ010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25934_length_2425_cov_2.840084"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKW73_10680"
     CDS             complement(<1..492)
                     /locus_tag="PKW73_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNA73797.1"
                     /translation="MKIEKVLIANRGEIALRVIRACRELDIRTVAVYSQADSESLHVK
                     LADEAYCIGPPQSVRSYLHIPAIISTAVGTGADAIHPGYGFLSEKANFAEICAVHQIK
                     FIGPSAHAINSMGDKSTARESMRKAGVPVVPGSHGLITDDDEAIRLASEIGYPVIIKA
                     TAGG"
     gene            complement(521..1066)
                     /gene="accB"
                     /locus_tag="PKW73_10685"
     CDS             complement(521..1066)
                     /gene="accB"
                     /locus_tag="PKW73_10685"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNA73798.1"
                     /translation="MQQIKELLGIVSSTDVTELTIEFGDQRITVKKEKQLATAFEFPV
                     ARDVRPVTMNMSPAVMNLESAYAASAPPAAQPTVAAKPEKEKVEAPAVSHEDNGSANT
                     VAITSPMVGTFYQSPSPTASAFVKVGDRINVGQTVCIIEAMKLMNDLPSEVSGKIVKI
                     CVDNGTTVEYGQTLFLVDPKG"
     gene            complement(1129..1689)
                     /gene="efp"
                     /locus_tag="PKW73_10690"
     CDS             complement(1129..1689)
                     /gene="efp"
                     /locus_tag="PKW73_10690"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNA73799.1"
                     /translation="MISSNDFRTGVTIEYNNGIWQVVEFMHVKPGKGAAFVRTRLKNV
                     ETGNVLEQTFRAGEKLPTANIEKSEFQYLYRSGENYTMMDQVSFEQMEMSANQIGSTV
                     EFMKEGLEGITVLRYNDRVIGVELPNTVELEVTEAAPDERGDTSSGGGKPATLETGAT
                     ITVPFHIKVGDKIKVDTRSRKYLTRV"
     gene            1935..>2425
                     /locus_tag="PKW73_10695"
     CDS             1935..>2425
                     /locus_tag="PKW73_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNA73800.1"
                     /translation="MTNSSTQAGETGAQAQAKGKAMKEDSNKASTAYVPSQVEEKWTA
                     YWHENKLFDLDVHPDKPRFSIALPPPNITGNLHMGHALNGTIQDVLIRLKRMQGYSVL
                     WQAGTDHAGIATQMVVERKLKAEGKNRHQMGREAFIEKVWEWRNQYGNQIMAQYKSLG
                     VSF"
BASE COUNT          598 a          627 c          625 g          575 t
ORIGIN      
        1 accaccggcg gttgccttga tgatgaccgg atagccaatt tcggaagcca gacgaatcgc
       61 ttcatcgtca tcggtaatca aaccgtgact gcccggcact acgggcacgc cggctttgcg
      121 catggattcc cgggcggtag acttatcgcc catggaattg atggcatggg cactgggtcc
      181 gatgaatttg atctggtgca cagcacagat ttcagcgaaa ttggccttct cggaaaggaa
      241 accgtagccg ggatgaatgg catcagcgcc ggtccccacc gccgtggaaa tgatggccgg
      301 tatgtgcaga taactgcgaa cgctctgggg cggtccgata cagtaggctt catcggccaa
      361 tttgacgtgc aatgactcgg aatcagcctg agaataaaca gccactgtcc taatgtcgag
      421 ttcgcggcaa gctctgatca cccgcagggc aatctcgccg cgattggcaa tcaatacctt
      481 ttcaatcttc atccggtaag cctttgattt agccttgttt ctagcctttg ggatcaacga
      541 ggaagagcgt ctgaccgtac tccacggtgg tgccattgtc gacacagatc ttgactattt
      601 tgccgctcac ttccgagggc agatcgttca tcagtttcat ggcttcgata atacaaactg
      661 tctgaccgac attgatgcga tcgcccactt tcacaaaggc ggaagcagta ggtgaaggag
      721 actggtagaa agtgccgacc atgggagaag taatagccac ggtgttggct gagccattat
      781 cttcatgact gacggccggt gcctctacct tctctttctc gggcttagcg gcaacggtag
      841 gctgtgccgc cggcggcgcg gaagcagcat aagccgactc aagattcatc acggccgggc
      901 tcatattcat ggtcaccggt cgaacgtccc tggctacagg aaattcaaag gcagtggcca
      961 attgcttttc cttcttaacc gtaattcttt gatcgccaaa ttcaatggtc agctctgtca
     1021 cgtcagtaga actgacgatg cccaatagct ccttgatctg ttgcaaatct attttcactg
     1081 ctctcgtcct gaaagtaatc tctgttgctc gctccgaaag gaaaatgctt agactctggt
     1141 caagtactta cggcttctgg tgtccacttt gattttgtcg ccgactttga tgtggaaagg
     1201 cactgtaata gtggcaccgg tctcgagagt agcgggcttg ccgccgccgg agctggtatc
     1261 gccgcgctcg tcgggagctg cttccgtcac ttccagttcc acggtgttgg gaagctcaac
     1321 gccgatgaca cggtcgttgt agcgcaacac agtgatgcct tccagacctt ccttcatgaa
     1381 ttccacggtg gaacctatct ggttggcgga catttccatt tgctcgaagg acacttgatc
     1441 catcatggtg taattttcac cggatctata aagatattgg aattcggact tttcaatatt
     1501 agcggtgggc agtttttcac cggcacggaa agtctgctcg agcacgttgc cggtctctac
     1561 gtttttaaga cgggtgcgaa caaaagcggc gccttttcct ggtttgacgt gcataaactc
     1621 aacaacctgc cagataccgt tgttgtattc gatggtgacg ccggtacgaa aatcattgct
     1681 tgaaatcatg acggtccaaa ttgctccaga aattgcgcga caaagagtag tcaagaagag
     1741 gtttccacct caagaccagg cagattctaa cattagatag atggtcactt acttttattg
     1801 ctaacggttg caaactaaag ccaaagaaag ggtcaagcac gccaagtatt aggcccacgc
     1861 tgttataatc gggcttccaa gagacatggt gccttttgtt ggaccatcaa tcccagcatt
     1921 cgagagaaaa gttaatgacc aatagttcga ctcaagcagg cgagaccggc gctcaagccc
     1981 aagcaaaagg gaaagccatg aaagaggatt ctaataaagc cagcacggct tacgttccca
     2041 gccaggtcga agagaaatgg accgcctact ggcacgagaa caagcttttc gacctcgacg
     2101 tgcaccctga caagccccgc ttttcaattg cgctgccgcc gcccaacatt acaggcaatt
     2161 tacatatggg ccatgccctg aacggaacaa ttcaagatgt tctgattcgc ctcaaacgta
     2221 tgcagggtta cagcgtgctc tggcaagccg gaaccgacca cgccggcatc gccacccaga
     2281 tggtggtgga acgcaaactc aaggccgagg gcaaaaatcg ccatcagatg ggtcgcgaag
     2341 ccttcataga aaaggtctgg gaatggcgca accagtacgg taaccagatt atggctcagt
     2401 acaagagcct gggtgtcagt ttctg
//
LOCUS       DAOQNZ010000576         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_25955_length_2424_cov_3.122415, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000576 DAOQNZ010000000
VERSION     DAOQNZ010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25955_length_2424_cov_3.122415"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1106
                     /locus_tag="PKW73_10700"
     CDS             132..1106
                     /locus_tag="PKW73_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73801.1"
                     /translation="MDELENLDDPLKTQIANLALPIKDDLLSGAAEIALRAISVFQTA
                     LNLAPADKKEDAPLLKSILTATAKALIDVQPAMAPLLHLGNSVLSAIDNATSVGEIKV
                     KTDEALTKFEYTLCASAATIADLAYDLIDPGELIFAYSFSSTVVSCLLNARARGRFFR
                     VVCTEARPSMEGRKLASRLASGGIEVIHTFDNAMGLVLGNCSAAFMGCDCIARPGVVN
                     KVGSWMLAIACRELKVPLYALSGTEKFIPDDRMFDFEKHERPAAEVWDNSPRGVAILN
                     HQFDLVPYDLLTGLVTEHGILKESDIEPYLVNSNSTMHRALVLEASAR"
     gene            1118..2221
                     /locus_tag="PKW73_10705"
     CDS             1118..2221
                     /locus_tag="PKW73_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA73802.1"
                     /translation="MRAIFDYPLPSFIYLSGAIIFLFCAFRFLKSQRNSRFLSYYFLA
                     TCVAYAILILLVILLPVQSFAAVTLLAWTIFAGGTILLALIIRQAIESQLKLTGGFYS
                     LILLVHLAITIDVFLIEPACLEIDRLNLQSEKLKREVKIALVTDLQTDHFGNYEKTAL
                     DKVLQEKPDLILLCGDYIQCMSKAEYDREGARLTEYLKESNFGAPLGAYAVRGNTELP
                     DWQDYFKGSRVETFEETTTFPKDQLVLTALNFYDSFNSKFDADLKKLAPAQTDRYHIV
                     FGHAPDFARSNTGGDLLLAGHTHGGQVCAPGFGPLVYLSTVERKWASGYNRIFGKRDL
                     VVSRGIGMERCYAPRLRFFCHPQLIIITVKPKI"
     gene            complement(2228..>2424)
                     /locus_tag="PKW73_10710"
     CDS             complement(2228..>2424)
                     /locus_tag="PKW73_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73803.1"
                     /translation="WVQGLMRLLWSEEEVYQRLEGLVNNVCTRVFDKARDKKLSLRMA
                     AMSLAVERIVEARKLRGLYP"
BASE COUNT          585 a          690 c          587 g          562 t
ORIGIN      
        1 cctatctcaa gaaacacaaa agtgaagccg ccctggccgg cgcttgagca gcggagtggg
       61 tgaaagattt aacgctgagc tagtttcact cgggctaaac tggatatatt gactgggaaa
      121 agaaggttgt catggatgaa ttagaaaact tggatgaccc tctcaaaacg caaatcgcga
      181 acctggccct gcccatcaaa gacgatctgc tctcaggcgc tgcggaaatc gctctgcggg
      241 ccatttcagt cttccagaca gcactcaatc tggctcccgc agataaaaaa gaagacgccc
      301 ccttactcaa atcgatcttg actgccacgg ccaaggcgct tatcgacgtg cagccggcca
      361 tggctccctt actgcacctc ggcaatagcg tcctgtccgc catcgataat gccaccagtg
      421 ttggcgagat aaaggtgaag accgacgagg ctcttaccaa gtttgaatac acgctctgcg
      481 cttcggcagc caccatcgcc gacctggcct acgatcttat cgatcccggc gagcttattt
      541 tcgcttattc ctttagttca accgtggtta gttgcctcct gaacgccaga gccagaggtc
      601 gctttttccg cgtcgtctgt accgaggccc gccccagtat ggaaggtcgt aagctggctt
      661 cgagactggc ttcaggcggc attgaagtaa ttcatacctt cgacaatgcc atgggtctgg
      721 ttttgggcaa ctgcagcgcc gccttcatgg gctgtgactg tatcgccaga ccgggagtgg
      781 tgaacaaggt gggttcctgg atgcttgcca ttgcctgccg ggagctaaaa gtgccgctat
      841 atgcactgtc cggcacggaa aaattcattc ccgacgatcg catgttcgac tttgaaaaac
      901 acgagcgccc ggccgctgaa gtctgggaca acagcccacg cggcgtggcc attctcaatc
      961 atcagttcga cctggttcct tacgacctct taaccggtct ggtaaccgag cacggcatct
     1021 taaaagagtc ggacatcgaa ccctacctgg tcaattccaa cagcactatg caccgggctc
     1081 tcgttttaga agccagcgct cgctgaaagg cgcactcttg cgtgccatat tcgactatcc
     1141 gctgccgtcc ttcatatatt taagcggcgc cataatattt ctcttctgcg cctttcgttt
     1201 cttgaaaagc cagagaaata gccgcttcct ctcctactac tttctggcaa cctgcgtcgc
     1261 ctatgccata ttgattctgc ttgtgatact actgccggtg cagtcctttg ccgccgttac
     1321 ccttttagcc tggactattt ttgcaggcgg caccatcctt ttggctctca taattcgcca
     1381 ggcaatagag agccaattga agctgacagg cgggttttac agcctcatcc tgcttgtgca
     1441 tctggccatc accatcgatg tcttcctgat cgagccggct tgcctggaaa ttgacagact
     1501 taatctccaa tcggaaaagc tcaaaagaga agtaaaaatc gccctggtta ccgacctgca
     1561 aaccgaccac ttcggcaact acgagaaaac ggctttagac aaagttctgc aagagaaacc
     1621 tgatcttatc ttgctctgcg gtgattacat tcaatgcatg agcaaagccg aatatgaccg
     1681 agaaggcgcc aggctgacag aatatctcaa ggaaagcaac ttcggagcac cgctgggagc
     1741 ttacgccgtg cgcggcaaca ctgagcttcc cgactggcag gactatttca agggcagccg
     1801 ggtagaaact ttcgaggaaa ccacaacctt ccctaaagac caattagttc taacggccct
     1861 caacttctac gactccttca atagcaagtt tgatgccgac ttgaagaaac tggctcccgc
     1921 gcagactgac cgctatcata tagttttcgg ccatgccccc gattttgccc gcagcaatac
     1981 aggcggcgat ttactgctgg ccggacatac acatggcggg caggtctgcg ccccgggctt
     2041 cgggcctctg gtctatcttt caacagtaga gagaaaatgg gccagcggtt acaaccgtat
     2101 cttcggcaaa agggatctgg tggtaagtcg gggcatcggc atggagagat gctacgcgcc
     2161 aagactacgc ttcttctgcc acccgcaatt gatcatcatc acggtgaagc cgaaaatctg
     2221 accagatcta agggtagagt ccacgcaatt tacgggcttc aacaatgcgc tccacggcaa
     2281 gcgacatggc tgccatgcgc aaggaaagct tcttatctct ggctttatca aagactctgg
     2341 tacaaacatt gttgaccaga ccttccaggc gctgatagac ctcttcttcc gaccagagca
     2401 agcgcatcaa accctgcacc catt
//
LOCUS       DAOQNZ010000577         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26008_length_2421_cov_5.608622, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000577 DAOQNZ010000000
VERSION     DAOQNZ010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26008_length_2421_cov_5.608622"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="PKW73_10715"
     CDS             <1..1713
                     /locus_tag="PKW73_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73804.1"
                     /translation="QADCLYRLAHGYSVSASKALQAFFYRVDDGEARKIFLSRMEQEH
                     WQYMALNGLSSAEDLLVLSCEMDAHFGTSLSESHFASIWKALASDEVKQYKLLGNILG
                     DPEPLDLRYAHTPSLVSANIKMRAVALADNFPPDQRERLLVYAAEDSCDDVSLFATQK
                     LMHYWSSPEGKNPPWLEPFPMLNLNLNFYLANMAAAFQWMDGEDDEEEVQTCLADLER
                     LRLGIKYAGEHMEHSLLATLKDQLAQVERQCQVLSQKRCGSLQKLVNRICAALDIPAA
                     IVDFTENNHMTACYLVGTGRIAVARDLLLTNEPLSEALMSTLLHEVLHMEQDVQTIRL
                     ICDSLDITYGQHSRELKQLMELYSESVGYAPEPIFLLAVLRMRDDRPLSPAQRSRAER
                     LYKGSLDSSHLHERATRLEARLEKIEKAKATLESGEKDCDLLLCLKDNRGLAQLFQKG
                     RVPLILLDELSDCKAEVERTLHELAAALPAEQREAFQSFKKDKIDLAIALFSQGVVKP
                     FAGTMKRIKHVLLQVIKEECRKLNRSLVITRRQGYHEDEAYYITDRTHIIVKALRKGW
                     YKMN"
     gene            complement(1710..2267)
                     /locus_tag="PKW73_10720"
     CDS             complement(1710..2267)
                     /locus_tag="PKW73_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73805.1"
                     /translation="MSFDLPLLTTESIIYGAALAGGLWLVYPRLANFIEYRVLSQVVK
                     AVTGHSTLRPRAIRVGENLSNSYDSVKRALASRNNAQRWQIRQVRFEEPIEKAIRLEG
                     LSVLKETPEEEMERIKKGGKRHSNSLRIQVDITETIQGTQVSWKFLPEDERYFNQRLQ
                     VMDASTSLLLLRTNFNIIRELGLTG"
BASE COUNT          607 a          673 c          626 g          515 t
ORIGIN      
        1 caggccgact gcctctatcg tctggcccac ggctattctg tatctgccag caaggcattg
       61 caggccttct tctaccgggt ggacgacggc gaagccagaa agattttcct cagtcgcatg
      121 gaacaagaac actggcaata catggccctg aacgggctgt cctcggcgga agacttactg
      181 gtactctcct gcgaaatgga cgcccatttc ggcacgagct tgagtgaaag ccacttcgcc
      241 tcaatctgga aggccctggc cagcgatgag gtaaaacagt acaaacttct cggcaatata
      301 ctaggcgacc cggagcccct cgatcttcgc tatgcgcaca caccatcttt agtctccgcc
      361 aacatcaaga tgcgcgccgt cgccctggcc gacaacttcc caccggatca aagagagcgg
      421 ctactagtct acgccgccga agatagttgc gacgatgttt cgctctttgc cacccagaag
      481 ctgatgcact attggagcag cccggaaggg aaaaatccgc cctggctgga acctttcccc
      541 atgctcaacc tgaacttgaa cttctacctg gccaacatgg cagcagcctt ccagtggatg
      601 gacggcgaag acgatgaaga ggaagtgcag acttgtctgg cggacttgga aaggctgaga
      661 ctgggcatca agtacgccgg cgagcacatg gaacacagcc tgctggccac gctcaaagat
      721 caactagccc aggttgagag gcaatgccag gtgctttcgc aaaaacgctg cggcagcttg
      781 caaaagctgg tcaatcgcat ctgcgccgcc ctcgacattc cagccgccat tgtcgacttc
      841 accgagaaca atcatatgac cgcctgctac ctggtaggaa ccggtcgtat tgccgttgcc
      901 agagatctct tattgacgaa cgagcccctt tcggaagccc tgatgtcgac cctcttgcac
      961 gaagtcttgc atatggaaca ggatgtgcaa accattcgcc ttatctgtga ttccctggat
     1021 atcacctatg gtcagcattc cagagagctg aaacaactga tggagcttta cagcgaatca
     1081 gtgggctatg ctccggagcc catcttcctg ctggcagtct tgcgtatgcg cgatgatcgc
     1141 cccctcagtc cggctcaacg ctcccgtgcc gaaaggttat ataaaggcag cctggactcc
     1201 tcgcacctgc atgaaagagc caccagattg gaagcacgcc tggaaaaaat agagaaagca
     1261 aaagccaccc tggagtccgg agaaaaagac tgcgatctgc tcctttgcct caaggataat
     1321 cgtggtctcg ctcagctctt ccagaaagga cgagtgcctt tgatccttct ggacgaactg
     1381 agcgactgca aggcggaagt tgaacgcacc ttgcacgaac tggcagcggc tctcccggca
     1441 gaacagagag aagccttcca gagcttcaag aaagacaaaa ttgacctggc tattgccctc
     1501 ttctctcagg gcgtagttaa accctttgcc ggcaccatga agcgcatcaa gcatgtgctc
     1561 ctgcaagtga ttaaagagga atgtcgcaag ctgaaccgct ccctggtcat taccagaagg
     1621 cagggctatc acgaagacga agcctactat ataaccgacc gcacccacat catagtcaaa
     1681 gctttgcgca agggctggta caagatgaac tagccggtca ggcccagttc tcgaatgatg
     1741 ttgaaattag tccgcaaaag tagaagtgat gtgctggcat ccattacctg caaacgttga
     1801 ttgaaataac gctcgtcctc gggcaggaac ttccatgaca cctgggtgcc ctgtatggtc
     1861 tcggttatgt ccacttgaat gcgcaaggaa ttggaatgac gtttaccgcc tttcttaatg
     1921 cgctccattt cctcttccgg cgtttccttg agaaccgaca gaccttccag acgaatggcc
     1981 ttctctattg gctcttcaaa gcgcacctgg cgaatctgcc agcgctgagc gttattgcga
     2041 gaagccagcg cacgcttgac ggaatcatag gaattgctca gattctcgcc aacgcggata
     2101 gcgcgagggc gcagggtgct gtgcccggta acggctttca ccacctggct caagacacgg
     2161 tactcgatga agttggccag gcgcggataa accaaccaca gaccgccagc gagagcggcg
     2221 ccgtagatga tggattcggt agtaagaaga ggtaaatcaa aagacatggc gttcctcagg
     2281 gtgaggtcgg gaaaaagtcg gacttgtctc cttcgcaagc aggaattgcc tcgcaaattg
     2341 caggctattc tatcattgtt cagctctagt aaatttctcg tgaaatcggc tttcgagaaa
     2401 tggtgggacc acgtatatag a
//
LOCUS       DAOQNZ010000578         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26106_length_2416_cov_3.220246, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000578 DAOQNZ010000000
VERSION     DAOQNZ010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26106_length_2416_cov_3.220246"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..546)
                     /gene="speD"
                     /locus_tag="PKW73_10725"
     CDS             complement(124..546)
                     /gene="speD"
                     /locus_tag="PKW73_10725"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNA73806.1"
                     /translation="MFGPHLILEAYGCPKETLADMNALSSILDAYPAQLDMTKIMPPY
                     VFTYHGTVEDDWGVSGIVLIAESHISIHTFPEKGFVTLDIFSCRDFDVDAAVDYFCSV
                     FKPESFDKEILMRGREFPRSMPKAQTIVASERRRLTAV"
     gene            624..794
                     /locus_tag="PKW73_10730"
     CDS             624..794
                     /locus_tag="PKW73_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73807.1"
                     /translation="MRSLGFSFIFPCGVHETLTYTRFVTTCLGVGRCVMRVNLLIESI
                     IEGRIVQEMFRL"
     gene            1094..1285
                     /locus_tag="PKW73_10735"
     CDS             1094..1285
                     /locus_tag="PKW73_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73808.1"
                     /translation="MLIRVAMDDSDWNEILKVLEQYEQTAQTEKAFQSTIRKSSINKA
                     IQTIRRVMQEQTTTLERQE"
     gene            1321..1725
                     /gene="mscL"
                     /locus_tag="PKW73_10740"
     CDS             1321..1725
                     /gene="mscL"
                     /locus_tag="PKW73_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNA73809.1"
                     /translation="MIKEFTDFLQKQNALALAIGVIIGGATGKVVTAIVNDLIMPLIG
                     LVMPAGDWRQAQFILSKSTDAAGAVTVNAIKYGDFIGSFVDFAIIAFVVFIITKAILP
                     KEAPPAPTKSCNACLETVPAAATKCKACTSPV"
     gene            complement(1763..>2416)
                     /locus_tag="PKW73_10745"
     CDS             complement(1763..>2416)
                     /locus_tag="PKW73_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73810.1"
                     /translation="VGDASEFSEAEMQTMEHELLGFYVTSHPLKRVANRLRYLTTHSI
                     KEMKEASDGTTVIVGGLANSIEKKLTKQNKLLCIVHLEDLTGKAEIVLYSEALEKVSS
                     DVLVPQSLLLIKGKVKKNEEQTSVMGSSIRRIADASMVDVVFTQTQSFADLHRLKDLL
                     ILHKGEDPVMLHFPQGQKSKAILVGAQFWVDATSSLPAAIETNFQESIKIRVNKVRV"
BASE COUNT          628 a          616 c          602 g          570 t
ORIGIN      
        1 tcctaaatac ttctaccacc tgctcctcta aaattccagc aaaccggaag ttgccggcga
       61 taattcctga aaaaagaaga ccccggctaa gccggggtct tctctctagc tctttctcag
      121 cctttagact gcagtcaagc ggcgacgctc gcttgccaca atagtctggg ctttgggcat
      181 gctgcggggg aattcccgac cgcgcatgag gatttccttg tcaaagctct ccggtttgaa
      241 gacgctgcag aagtaatcaa cagcagcatc aacatcgaaa tcgcggcaag agaaaatgtc
      301 gagggtgaca aatccttttt ccgggaaggt gtggatggag atgtgtgact cggcgatgag
      361 cacgataccg ctaactcccc agtcgtcttc cacggtaccg tggtaagtga agacataggg
      421 cggcataatc ttggtcatat ccagctgagc cggataagcg tcaagaatac tacttagagc
      481 attcatgtct gccaaagttt ctttgggaca gccatatgct tcaagaatta agtgtggtcc
      541 gaacattgtt tcttccccct tgtgccgtct gggtgtattc agacggtaaa tagaatgggc
      601 taccgttgtc cagggagaaa agaatgagaa gccttggctt ttcctttatt tttccctgtg
      661 gggtacacga aacgcttact tacaccagat tcgtaacaac ctgcctggga gttgggcgtt
      721 gtgtgatgcg agtcaacctc ctcatcgaaa gtattatcga aggacgtatt gtacaggaaa
      781 tgttccgcct gtaaacccct ttggagcagg tgtgaacctg ggtaacggga ctttttcaag
      841 acaggctaaa caagctactt tcaaccagca taaaaagtct cgaaatccaa cccgaccact
      901 ggaattgggc acaaatttaa ggaaattcag gaggctgcct aaaatctccg cccgatcttc
      961 ccacctgaca gggcacaggc cctatactga aacgcccttt cctggtagaa atggcaacta
     1021 acccttgcgg ccaatcgcaa gcagccgccg ggagagcact ggaagaagca gtcggagaaa
     1081 gagcagaccc aatatgttaa taagagtagc gatggacgac tcggactgga acgagatttt
     1141 gaaagtgctc gaacaatacg agcagacggc tcagaccgaa aaagcatttc agtcaaccat
     1201 aaggaaatcc agtatcaata aggcgattca gacgattcgc cgggtgatgc aggaacaaac
     1261 caccaccctg gaaagacaag aatagattca aagtacggac agaacctggg agactctcaa
     1321 ttgatcaagg aatttacaga ctttcttcag aagcaaaacg ctctcgctct cgctatcggt
     1381 gtcatcatcg gcggcgccac cggcaaagta gtaacggcca tagtgaacga tttgattatg
     1441 cctttgatcg gtctggtcat gccggccggc gactggcgcc aggcacagtt catactctcg
     1501 aaaagcaccg atgctgccgg agccgtcact gtcaatgcca tcaaatacgg tgatttcatc
     1561 ggttccttcg tcgactttgc catcatcgcc ttcgtggtct ttataattac caaggctatc
     1621 ttgcctaaag aagcgccgcc ggctcccacc aagagctgca atgcctgcct ggagaccgtg
     1681 ccggccgccg ccaccaagtg taaagcctgc acttctcccg tctaagaagg caagcccgtc
     1741 ggatatttgc gggtctgaaa cttcagaccc tgactttgtt tacccttatt ttgatgcttt
     1801 cctggaaatt ggtttctatg gccgccggca ggctggatgt agcatcgacc cagaactgag
     1861 cgccaacaag aatggccttg ctcttctgcc cctgggggaa atgcagcatg accggatctt
     1921 cccctttgtg caaaatcaaa aggtctttca atctatgcag atctgcaaaa gactgggttt
     1981 gagtgaaaac cacatcgacc atggaagcat cggcgatgcg cctgatggat gaacccataa
     2041 ccgaggtctg ttcttcgttt ttcttgacct ttcctttgat aaggagcaag gactgcggca
     2101 caagcacatc ggaagagacc ttctcaaggg cttcgctata gagaacaatc tcggccttgc
     2161 cggtcaaatc ttccaggtgc acaatgcaca gaagcttgtt ttgcttggtc agtttcttct
     2221 ctatggaatt agccagcccg ccgacaataa cagtagtgcc gtcggaggct tccttcatct
     2281 ctttgatgga atgggtagtg agatagcgca gtcgattggc aacccgcttc agggggtgac
     2341 tggtgacgta gaaaccgaga agctcgtgct ccatggtctg catttcggct tcggagaatt
     2401 cggaagcatc gccaac
//
LOCUS       DAOQNZ010000579         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26189_length_2412_cov_3.331353, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000579 DAOQNZ010000000
VERSION     DAOQNZ010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26189_length_2412_cov_3.331353"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..>2412
                     /locus_tag="PKW73_10750"
     CDS             158..>2412
                     /locus_tag="PKW73_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA73811.1"
                     /translation="MNSLLSDDASNQNRYANLKLLHRGRKHLIFRALRTSDNLPVVIK
                     TLALNPTSTTLAADLKKEYERLKSLHLAGSARIIDFEQFQGKPALIMEDAGPLNLEGY
                     LTERRRQKSAADLPTLLPLALSMTECLNRVHKAGLLHLDLCPANFVLNDALQPTGVTI
                     VDFGAAHQANQTAEEPIDPISLDGTLAYLSPEQTGRMNQPVDFPSDFYSLGAIFYEMF
                     TGNPPFPLQDPLELVHAHLSISPVPPHQLNASVPEVLSQLVLKLLNKRPEDRYQSAYG
                     LACDLERIINMQEMGEQIASFHLGLEDRPYGLQIPSRLYGREMETAALLQAFHNTIEE
                     GRAELFLVGGYSGVGKTTLVNELYKPLIQEEGFFLSGKFDQYRTNVPFATFHQAFQGF
                     IHYLLTKPDTIIEHWRNELEKALGQNAAVILNVIPELELLLGEKPTVPTLSTAEEQLR
                     FDLTFSKFVSVFARSEKPMAIFLDDLQWADAASLRLIKNLVSEEGIGTLLIIGAYRDN
                     EVGPEHPLSQMLKELAESEIAVNQVILKPLSEKQACALVADTLRCSELEAAPLSGLVY
                     EKTNGNPFFTIQFLNTLYQEQLLFFDDGGRTWKWDLAQVEAQGYADNIVDLLLAKLLR
                     LPAAVREILKLAACLGNKGDFRTLSILCQQDEATLIENIQPAIEQGLILNQSGTYKFL
                     HDRVQQAAYSLIPEGERSQEHLRIGHLLLQHSKNEEVEERVFDIVNHLNLGKAHITET
                     EELLALAALNLV"
BASE COUNT          621 a          685 c          581 g          525 t
ORIGIN      
        1 acctaaatcc cagcttaccg ccctttcccc agctttacaa caaaatcgtc agggcggtaa
       61 actgccccgg tggagacaca agcgctataa tctagagtcg gccgcaagag cctctggtgg
      121 agtcctgcga ttactttgca acacagttgg ttctgccatg aattcgctat tgagcgacga
      181 tgcttcaaat caaaatagat acgctaacct gaagcttttg caccgtgggc gcaaacactt
      241 gatttttcgc gccctgagaa ctagcgataa cctgcctgta gtcatcaaga ctctggctct
      301 caatccgacc agcaccaccc tggctgccga cctgaaaaaa gagtacgaac gactcaagag
      361 cctgcactta gccggttctg ccagaatcat cgatttcgaa cagtttcaag gcaagccggc
      421 ccttatcatg gaagatgccg ggccactcaa cctggaagga tatctgacgg aaaggagaag
      481 gcaaaagtcg gcagcggatc tgccaactct tctgcccctg gccttgagca tgacggagtg
      541 cctgaatcgc gttcacaaag ccggtcttct gcaccttgat ctttgcccag caaattttgt
      601 tctgaacgat gccttgcaac caacaggcgt cactatcgta gatttcggcg ccgcccatca
      661 ggcaaatcaa acggcagaag aaccgatcga ccccatcagt ctggatggga ccctggccta
      721 cttatcgccg gagcaaaccg ggcgcatgaa tcagcctgtg gacttcccca gtgattttta
      781 cagcctggga gcgattttct acgagatgtt cacgggcaac ccaccctttc ccctgcaaga
      841 tccgctggaa ctcgtccatg ctcatctctc gatttcgccg gtgcctcccc atcagttgaa
      901 tgccagtgtg ccggaagtgc tctcacaact ggtgctcaaa ctactaaata agcgtcccga
      961 ggatcgttat caaagtgcct atggtctggc ctgcgatctg gagagaatca tcaatatgca
     1021 agagatggga gagcaaattg cttcctttca cctcggtctg gaggatagac cctacggcct
     1081 acaaattcct tcgcgcctct acgggcggga aatggagacg gccgcgcttc tacaagcctt
     1141 ccacaatact atcgaagaag ggcgagccga gttgtttctg gtgggaggtt attccggggt
     1201 aggcaaaacc accctggtca atgagctgta caaacccctc atacaagagg aaggattctt
     1261 tctttccggc aaattcgatc agtaccgaac caacgtacct tttgccacct tccaccaggc
     1321 tttccagggc ttcattcact accttctcac caaacccgat accatcatag agcactggcg
     1381 aaacgaactg gagaaagccc tcgggcaaaa tgccgccgtc atattgaacg taattcccga
     1441 actggaactt ttgctggggg aaaagccgac ggtgccgact ctcagcaccg ccgaagaaca
     1501 gttgcgcttc gacctcacct tcagcaagtt cgtcagcgtc tttgccagaa gcgaaaagcc
     1561 aatggctata tttttagacg atttgcagtg ggccgatgcc gccagccttc gcctcatcaa
     1621 gaatctggtc tcggaagagg gaattgggac attgcttatc atcggagcct accgagacaa
     1681 tgaggtaggc cccgagcatc cgctcagtca gatgctgaaa gaactggcag agagcgaaat
     1741 agcagtaaac caggttatcc tcaagccgct ttccgaaaag caagcctgcg cgctggtggc
     1801 cgatacgctg cgctgcagcg aattagaagc agcgcccctg tcaggtcttg tttatgagaa
     1861 gacaaacggc aatcccttct ttaccattca gtttctcaat acgctctatc aggagcaact
     1921 cctctttttc gacgacggcg gccgcacctg gaaatgggat ctagcccagg tagaagccca
     1981 gggctatgcc gacaatatcg tagacttgtt gttggcaaaa ttattgcgtt tacccgcggc
     2041 cgtcagagag atactaaaac tagcagcctg cctgggcaac aaaggcgact tcagaaccct
     2101 ctccattctc tgccagcaag acgaagcgac gctcatcgaa aacatacaac cggccataga
     2161 acaagggctc atactcaatc aatcaggcac ctataaattt ctccatgacc gggtgcagca
     2221 ggctgcctat tcactgattc cagaggggga gcgcagccag gagcacttgc gcatcggaca
     2281 cttgctattg caacactcta agaacgaaga ggtcgaggag cgcgtcttcg acatagtcaa
     2341 ccacctcaat cttggtaaag cgcatatcac cgaaaccgag gaattactgg ctctggctgc
     2401 cctcaatctg gt
//
LOCUS       DAOQNZ010000580         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26270_length_2409_cov_3.314359, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000580 DAOQNZ010000000
VERSION     DAOQNZ010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26270_length_2409_cov_3.314359"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKW73_10755"
     CDS             <1..211
                     /locus_tag="PKW73_10755"
                     /inference="COORDINATES: protein motif:HMM:NF021641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA73812.1"
                     /translation="PIFWQFIGLGGSSYGVLERFDELTGRYVDNAGFFAIDDYNSIAD
                     EELYSRIIKEFPKWLEKVRGLGMLP"
     gene            363..1328
                     /locus_tag="PKW73_10760"
     CDS             363..1328
                     /locus_tag="PKW73_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNA73813.1"
                     /translation="MSYPNWLKAIFAFIAARLAALRKQINYLRDKLDEHLVRRQLTFA
                     QLSLQAIRFVLNRHEILSRASAIAYGAMLAFLPFITLVITATALFLPDLAGAGQASKD
                     LAFMLATIFPADVTSLILDQLQQIQKQPTLPVLGVTLLLAIWTATSLYSEIINSLNRI
                     LGVNESRPYWRLQLTSASLALMQAVVLTTAFVAVVIWPHLVQFLSLSGWSAGFATILQ
                     WCLLFGVLHLSFAIVFHFGPANQKGNKKLTHWVSPGATFGTIVFLLATSGFRFYLEHF
                     ANYRTAYGSLGGLMMLMVWLWVMSLVLLISAEINKLAALAEGKKP"
     gene            complement(1524..1754)
                     /locus_tag="PKW73_10765"
     CDS             complement(1524..1754)
                     /locus_tag="PKW73_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73814.1"
                     /translation="MSGNGPETLECTYIHKLKDNVEEIPVTESMFGPALLVCPGDKDY
                     PFKNSSKNSSAHLESGKELEKKGVLSNLQLDG"
BASE COUNT          587 a          695 c          550 g          577 t
ORIGIN      
        1 ccccatcttc tggcagttca tcggtctggg cggcagcagc tacggcgtgc tggagcgctt
       61 cgacgaattg accggacgct acgtcgataa cgccggcttc ttcgccatcg acgactacaa
      121 ctccatcgcc gacgaagaac tctacagccg catcatcaag gaattcccca agtggctgga
      181 aaaggtgcgc ggtctgggca tgctgccctg aaacaaacat agatagccgc tcagtagtgg
      241 ttttcactct taactaaaga gcgccggtaa gacgcacttg tttgcagttg cagcaagccg
      301 tcttcaccgg cctcccgtga tacggaaagt gtcgcgagag cgaataagaa tatggagtca
      361 gaatgtcata cccgaattgg ctaaaggcca tcttcgcttt tatcgctgct cggctggcgg
      421 ctttgcgcaa acaaatcaac tacctgcgcg acaagctgga cgaacacctg gtcagaaggc
      481 aactcacctt cgcccaactg tcactgcaag ccatccgctt tgtcctgaac aggcacgaga
      541 tattgtctcg cgcctctgcc atagcctacg gagcgatgct ggcttttcta ccatttatca
      601 ccctggtcat tacggctacc gccctcttcc ttcccgatct ggccggggcc gggcaagctt
      661 ccaaagacct ggctttcatg ctcgcgacca tctttcctgc agatgttacc agcctgattc
      721 tcgatcagtt gcaacagatt cagaagcagc ccactctgcc ggttctcggt gtgacgcttt
      781 tgctggccat ctggaccgcc accagtctct actcggaaat aatcaactcc ctaaaccgca
      841 ttctgggagt aaacgaaagc cgtccctact ggcgcctgca actgacttcg gccagcctgg
      901 ccctcatgca agcggtagtg cttaccaccg cctttgtagc agtggtgatc tggccgcacc
      961 tggtgcaatt cctgagcttg agcggttgga gcgcaggctt cgccaccatc ttgcagtggt
     1021 gtctgctctt cggtgttctt catctcagct ttgccattgt ctttcacttt ggaccggcca
     1081 accagaaagg gaacaagaag ctcacgcact gggtttcccc cggtgccact ttcggcacca
     1141 tcgtcttctt gcttgccacc tcgggttttc gtttctacct tgagcacttc gccaactacc
     1201 gtacagccta cggctccctc ggtggtctga tgatgctcat ggtctggctc tgggtgatga
     1261 gtctggtctt gctgatcagt gccgagataa acaaactggc ggcgctggcg gaaggcaaga
     1321 aaccgtaaag ggaagtcgtt taacaccacc agcggggcgc cttgttacac gaatacggat
     1381 acggacaagt gcacgcgcac aaggcacaag cagatgcaca agcgcgcgcc atcagagaga
     1441 gccggtcaaa ccctcgcgga agcgacttgc tctctttttt ttcatcctat agtcgaccgg
     1501 tctactggta tctattttag acactagcca tccagttgca aatttgacag tacacctttt
     1561 ttctccagct cctttccact ctcaaggtgc gcactggaat tcttactgga attcttaaaa
     1621 ggatagtcct tatcgcccgg gcaaaccagt aaggctggtc caaacataga ctcggtaact
     1681 ggaatttcct caacgttgtc tttcagctta tgaatgtaag tacactcaag agtttctggt
     1741 ccattaccac tcatcgccac gcactccata gaaagatcaa atctcacaga ttacagattg
     1801 caatctgaca ctcccgaatt gttcagtttc cgccatcatc tgacacgaaa actaccatga
     1861 ttttatggcg gagttgtggt gaaattcagg aattaaaaca atcacctcag ttattgccat
     1921 gctcgcttta gcaatccgct aggcccaagg ttttggccga cctgaaagac aggtaccggc
     1981 cccatgccaa aaaccgatcg cctctagaac acttcttagc gaaaaaccga gacagttgga
     2041 gccatccaag caagagtgcg tacacagcca ctatcagctt ttctaattct tgcccatcgc
     2101 acaggcaagg ccatggacga ccactctgct aatgcagtgc tgacggcgcc gacgaaacta
     2161 ccaggaaaag ccagggagga acaattcacg cttgcaaaga ggattcgtta acagccagac
     2221 tgaattgtaa ttcgctttct cctagtttca aaaccaaaat ctactccaac ttaaattcaa
     2281 ccaccaccac cgccattttt tctgccagcg cctgagcaat cggcaccggc agcccagcat
     2341 atctatatgc gaaagcgaaa taatttcacg gtcatttttt gcaaataatc ttggacaaaa
     2401 aatatggac
//
LOCUS       DAOQNZ010000581         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26305_length_2407_cov_3.457483, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000581 DAOQNZ010000000
VERSION     DAOQNZ010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26305_length_2407_cov_3.457483"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..393
                     /gene="ffs"
                     /locus_tag="PKW73_10770"
     ncRNA           301..393
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKW73_10770"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            431..706
                     /locus_tag="PKW73_10775"
     CDS             431..706
                     /locus_tag="PKW73_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73815.1"
                     /translation="MSKSSKSPKGNNDNHDQEPSRTLLGQILVELDYITIYQLDEALR
                     IQREDKDCGREAKPLGQILLELGYCGPNQLIRAIQVQAEYRKAQASK"
     gene            812..>2407
                     /locus_tag="PKW73_10780"
     CDS             812..>2407
                     /locus_tag="PKW73_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73816.1"
                     /translation="MNLSSRLTFALVTVVIVVVFGHVAYSVYLEYRSTRTEAGISLSD
                     PNDKEGRCAKLKLLGSLQSTQTFTDLVARKLLKDNKVKTGLDDRDKNLLAQAAKETAE
                     REIFAGSIWLLDEKGNVLFNSDTPQKSGYSLKQDSQGLDVAISTKQEFHGTLSNNQTG
                     MVSVSTILPAPHGFIVVNQPISQEYLSAIATRIGIENPRAQGVELAIYSRRQSKTVAW
                     SQDFPQQKCFRFLQEINLKGLPQGYPEKPFAAITGISFIDQLLVPAPGFERADGWWID
                     FRTLDKSGEFNAVIFLAKPVPSMTSKAVEVVLLGGLVGVLGVLVGIFLATRIAASVNE
                     PMKFLIKRTRAIANNKQVIPPLEGLTGDWYDLGELIDTAVLSMRSTTQNLKTQLTKQM
                     KQVEERNQLAEQSTQQVDVLNNQITHQAKQIAEYSRQINQANRQAIMVQQQLDAVLQA
                     STEGYLITDQFGNILHANPIVLNWLGTTEGEIAGRLCFDLVRKPGEPSHMESPGQAFA
                     QHGGNANALIEQFYPEGVVHSKDG"
BASE COUNT          641 a          671 c          579 g          516 t
ORIGIN      
        1 ggataaccca actgccatgc aatagcctgg ttatctcttc cggcggcatg aggtgcccct
       61 cggccgacac ctttaatcct tgccaatcgc cagttgtcat tgcctttggg actcctgatt
      121 cgcaagagaa catttggtgc tgtaggtctg atttgattca ataaaatcaa caattacaaa
      181 gtatgcgaca gaattgcctc tttgctatta gaaatagaga aaatttgcct ggattgcaaa
      241 acaatctatt aacactagac tcccttttag cagtccgctt gcactataat ggtcaatcac
      301 agatcgttgg gcgtggcggg agccgacgaa cctggtcagg ggggaaaccc agcagccata
      361 agttgaactt tcgggtgcgt ccagcggtct gctatctaaa agtaaagtca aagtcaaact
      421 cagagctaag atgtcaaaat cgtccaagag ccccaagggt aataacgaca accacgatca
      481 ggaaccgagc cgtacccttc tgggtcaaat tctcgtggag cttgactaca taaccatcta
      541 tcagctcgat gaagctctgc gcatccagag agaagacaaa gattgcggtc gtgaagccaa
      601 gcccctcggt cagattttgc tggagctggg ctactgtggt cctaaccaat taatcagagc
      661 catccaggtt caagccgaat atcgcaaagc gcaagccagc aagtaattca gcaagcgcta
      721 aatacactta tcgggttaaa cttctagttg tgtccgtcta tgtctttgca gatatagatt
      781 tatccgtcaa gcgtcagggt caaacttcta gttgaatctc agtagtcgtc tcactttcgc
      841 actagtaact gtagtaattg tcgttgtctt cggacacgta gcttatagcg tctatcttga
      901 gtaccgcagc accagaacgg aagccggcat cagcctctcc gaccccaacg ataaggaagg
      961 tcgctgtgcc aagctgaagc tacttggcag cctacaaagc acccagactt tcaccgacct
     1021 ggtggctcgc aagctcttaa aagataacaa ggtcaaaacc ggtcttgacg accgggacaa
     1081 gaatctgttg gcccaggcag cgaaagaaac tgctgagcgc gaaattttcg ccggctccat
     1141 ctggctcctg gatgaaaagg gaaatgtcct ctttaacagc gacacacccc agaagtccgg
     1201 ttattccctg aagcaagaca gtcagggtct ggatgtagcc atttccacca aacaagaatt
     1261 ccacggcacc ctctccaaca accagaccgg catggtctca gtcagcacca tcctgcctgc
     1321 gccccacggc ttcattgtgg taaaccagcc aatctcgcag gaatatctct cggccatcgc
     1381 cacccgcatc ggcattgaga atccacgggc ccagggtgtg gagctggcta tttattccag
     1441 aagacagagc aaaaccgtgg cctggtctca ggacttcccc cagcaaaagt gttttcgctt
     1501 cctccaggaa ataaatttga agggtctgcc ccagggctac ccggaaaagc ctttcgcagc
     1561 catcaccggc atatcattta ttgatcagct cctggtaccg gcaccgggct ttgaaagagc
     1621 ggacggctgg tggattgact ttcgcaccct ggataaatca ggcgaattca acgccgtcat
     1681 cttcctggcc aagccagtac ccagcatgac cagtaaggcc gtggaagtgg tgctgctggg
     1741 cggactggta ggcgttctgg gagtattggt gggaattttc ctggccactc gcattgccgc
     1801 ctccgtcaac gaacccatga agtttctcat caagcgcacc cgggccattg ccaacaacaa
     1861 gcaagtaatt ccgcctctgg aagggctcac cggcgactgg tacgaccttg gcgaattaat
     1921 agatacggca gtactgtcca tgcgctccac cacccagaac ctgaaaactc agttgaccaa
     1981 acagatgaag caggtggaag agcgcaatca actggccgaa cagtccaccc agcaagtgga
     2041 tgtactcaat aaccagatca cgcaccaggc caagcagata gctgaatact caaggcaaat
     2101 caatcaggcc aatcgccagg ccatcatggt gcagcaacaa ctggacgccg tcttgcaagc
     2161 cagtacggaa ggctatttga tcacggatca attcggcaac atattgcacg ccaaccccat
     2221 cgtcctcaat tggctaggta ccactgaagg tgaaatcgcc ggccgactct gcttcgatct
     2281 ggtgcgcaaa ccgggcgaac cttcccatat ggaaagcccc ggacaggctt tcgcccagca
     2341 cggcggcaat gccaacgccc tcatcgagca gttctacccc gaaggcgtgg tgcactcaaa
     2401 agacggc
//
LOCUS       DAOQNZ010000582         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26316_length_2406_cov_4.494258, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000582 DAOQNZ010000000
VERSION     DAOQNZ010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26316_length_2406_cov_4.494258"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..739)
                     /gene="atpH"
                     /locus_tag="PKW73_10785"
     CDS             complement(194..739)
                     /gene="atpH"
                     /locus_tag="PKW73_10785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNA73817.1"
                     /translation="MNTELQGIAAQYSEAVLELAQAAGQDEVVLTELNAVNQVIASDK
                     DMTIVLCHPSISAEDKKKFLASLFQGKLSDLTDKLINLLADKRRLELLPFIESSYRSL
                     LNKRKNILSASLRCSEPLADSSIANIKAQLTEHLGKKLELEVKVDPSLIGGVVLKIGD
                     QVIDGSLKGKLKSIEKALMSV"
     gene            complement(762..1316)
                     /locus_tag="PKW73_10790"
     CDS             complement(762..1316)
                     /locus_tag="PKW73_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNA73818.1"
                     /translation="MFFAEVEAPGFMSIFENNLINWLLLVAFLWWVLAKNLPPVFKGR
                     EESINSTLNAAKEARAQAEALLAKQKAAVANAEKEAQEILAEARAAAKDMQESMEAQT
                     KKDIAEMLVKFENAIAAERQMMVTEMRQASVKAAMELAKGQLASAVTPEVKSQMLNQF
                     MEQLETLNTRSQSMPTSASLSATK"
     gene            complement(1316..1795)
                     /locus_tag="PKW73_10795"
     CDS             complement(1316..1795)
                     /locus_tag="PKW73_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNA73819.1"
                     /translation="MLEVNGTLLILVVSFLAFIWMLDMVFVTPVSKTLEARSRKIQED
                     LDNSKDLRKEAEAVLSQYEKHLAEVREKAQATINDAVGEAQKKRSEAVSKIQDEGRGR
                     LDAAKASLASEKRSLVEQMVDEQAQLVDAIMGKLIGSSHRAGNLDKGQVKRSLEEVC"
     gene            complement(1911..2201)
                     /gene="atpE"
                     /locus_tag="PKW73_10800"
     CDS             complement(1911..2201)
                     /gene="atpE"
                     /locus_tag="PKW73_10800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNA73820.1"
                     /translation="MDHQLIAQAGTMAGGVDNVTLIKCAALLGAGLGAIGVGVPGIGI
                     GIAASRALEGMARQPEMQGKLLINGIIFAALMEALGLFAFLVSLQLFGLGSK"
BASE COUNT          523 a          645 c          604 g          634 t
ORIGIN      
        1 ttccatgccg tagatacgag cgataccgtc acccacctgc agtacgctac cgacattggt
       61 aacgttcagg gatgagcggt atttctccaa ctggcttttg agaactgatg tgatttcgtc
      121 gggcctgatt gccatggttt cttaacctct aatttcctaa ctaaccttaa aagagaccga
      181 tttttggtgc caactaaaca gacataagag ccttctcgat ggacttcaac ttgcctttga
      241 ggctgccatc gattacctgg tcgcctatct tgagcacgac accacctatc agggaggggt
      301 cgacttttac ttccaattcc agctttttgc cgaggtgctc ggtaagctgg gctttgatat
      361 tggcgatgga agagtccgcc aggggctcgg aacatctcaa ggaagcggaa agaatgttct
      421 tgcgcttatt gagaagggag cggtaactac tttcgatgaa gggcaaaagt tccagtcttc
      481 tcttatcggc aagcaagttg atgagcttgt cggtgaggtc ggagagcttg ccctggaaga
      541 gcgaagcgag gaactttttc ttgtcttccg ctgagatgga tggatgacag agcactatag
      601 tcatgtcttt atcggaagca atgacttgat tgacggcatt gagctcggtc agtaccactt
      661 cgtcttgacc ggctgcctga gccagttcaa gcaccgcttc ggagtactgg gctgcaattc
      721 cttgtagttc ggtattcatc ttttactact tacctgcccc tttacttggt tgccgaaagc
      781 gaagcgcttg taggcatcga ctgagaacgg gtattgaggg tctccagttg ctccatgaac
      841 tgattgagca tctggctttt tacttccggt gtcacggccg acgccagttg tcccttagcc
      901 agttccatcg ctgctttaac tgatgcctgg cgcatttccg tcaccatcat ctgccgctcg
      961 gcggcgatgg cgttttcaaa tttgacaagc atttcagcaa tgtctttttt ggtttgtgct
     1021 tccatcgact cttgcatgtc tttggcagcg gctctggctt ccgccagtat ttcctgcgct
     1081 tccttctcgg cgttggccac agccgccttc tgtttggcca gcagggcttc agcctgagcc
     1141 cgggcttctt tggcggcgtt caaggtagag tttatgctct cctcgcgacc tttaaagacc
     1201 ggcggcaggt tctttgccag gacccaccag agaaaggcaa ccagcaaaag ccagttgatc
     1261 aagttatttt caaagatact cataaagccc ggagcttcca cttcggcaaa aaacatcagc
     1321 agacctcctc tagtgaacgc ttgacctgac ctttatcaag gttgccggcc cggtgtgatg
     1381 aaccaatcaa tttgcccatg atggcatcta ccaactgagc ctgttcatcc accatttgct
     1441 ccaccaggga gcgtttctcg gaagcgagag aggctttggc ggcatcgagg cgaccgcgac
     1501 cttcatcttg aatcttagaa accgcttcgc tgcgtttctt ttgcgcttca ccgacagcat
     1561 cattgatggt ggcttgcgct ttttcacgca cttcggcgag gtgtttctcg tactggctca
     1621 acaccgcttc tgcttctttg cgcagatcct tggagttgtc caggtcttcc tgaatctttc
     1681 tggagcgtgc ttccagagtc ttggatacgg gggtgacgaa gaccatgtcc agcatccaaa
     1741 tgaaagccag gaagctcacc accagtatca ataatgtgcc gtttacttcg agcatttttg
     1801 caaaatctct atttcttccg tcgactctaa gccttagtct caccgtcggg ccacagaggg
     1861 cccatagcac agctcctccc caagaggagg agctgtctca attagattga ctatttgctt
     1921 cccaatccga acaactggag ggagacgagg aaggcgaaga gaccgagagc ttccatcaag
     1981 gcggcgaaga tgatgccgtt gatgagcagc ttaccctgca tttcaggttg acgagccatc
     2041 ccttcgaggg ctctagacgc tgcgataccg ataccgatac caggaacgcc gacgccgatg
     2101 gcgccgagac cagcgccgag aagcgctgcg cacttgatca aggtaacgtt gtcaacaccg
     2161 cccgccattg ttccagcctg ggcaatcaat tggtggtcca ctgcatttct ccttcaatat
     2221 gaccgaaaat tcgccagctg cctcagcttc caaggcagag cttatgctcc acctcaggat
     2281 tgccgccgca tttgctgcaa cttgcaccct agtggttctg caaaaggcgt acaaattata
     2341 tcaggatgaa agtgccttcc tgtgccggtg agagaccgtt aagcctggct cttggcgcat
     2401 ggatac
//
LOCUS       DAOQNZ010000583         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26373_length_2404_cov_2.919540, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000583 DAOQNZ010000000
VERSION     DAOQNZ010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26373_length_2404_cov_2.919540"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..603)
                     /locus_tag="PKW73_10805"
     CDS             complement(88..603)
                     /locus_tag="PKW73_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73821.1"
                     /translation="MKLQKLASKTGPLLLALTVSLGAGLTPASAQSSAGGKAEDRCRE
                     KMVKLQGQGNSQFKNKNFAAGDASFRQALNEAKKLGKAESALALQRWAQATEFQRADL
                     KRAELLYMEAVSLVRNEPKDAHLRAVQVLAQFYRRHNQTDKADLQEQIAQQLQGGQSI
                     ERDPCPACGRG"
     gene            complement(659..865)
                     /locus_tag="PKW73_10810"
     CDS             complement(659..865)
                     /locus_tag="PKW73_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73822.1"
                     /translation="MSTALKHYQERLLELLETKLNAQEVKQALLLDAELAQLHTYIET
                     LDLDMLTVGMELQKKWGKKQERQN"
     gene            complement(867..>1226)
                     /locus_tag="PKW73_10815"
     CDS             complement(867..>1226)
                     /locus_tag="PKW73_10815"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNA73823.1"
                     /translation="TNGFLVLDLHNLYCQMVNFNLTLAEILFYYPLELVKELHISGGS
                     WWEPEISHSRRIRRDTHDGNVPPQVMALIGEVVPLCPALKWIILERMGGTICTREDES
                     EFVQQFMEIRQQVKSAK"
     assembly_gap    1227..1326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1327..1646)
                     /locus_tag="PKW73_10820"
     CDS             complement(<1327..1646)
                     /locus_tag="PKW73_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73824.1"
                     /translation="MSEQTQKTNRTSPRVGLSLMPTEDFRKASLPLLQDNLVECLEWT
                     FDAGFDRAIPLWVENLLAKFSEQESLLGHSFFLSPMSLVRPPEIEESLKKFSLACTLH
                     KYQH"
     gene            complement(1649..>2404)
                     /locus_tag="PKW73_10825"
     CDS             complement(1649..>2404)
                     /locus_tag="PKW73_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73825.1"
                     /translation="PVVPFYISFLVMKLSQSNLIVVSLLALSLNAHYPAAEVLAAPQT
                     SKQDKSVKPEAPAKQAPPVKTDAPQTSKQDKAVKPEKPVPAKSQAQATAVKATGKEKP
                     AQAKGKSASRPRTTMLVPPPPPMTPSLLVDPSMQAVLGMTMPVEMLGKETLKEREKEV
                     AIQYKDAALELEAKQRKLKDKEDRAVSFEELYKEGVVSRRELEAAQAEAKEFVVDVER
                     LAIKEHELKLLLERINKRLAALSPKPGKKSLLR"
BASE COUNT          452 a          551 c          603 g          698 t
ORIGIN      
        1 attaattctg agttttcgct ctcatttaat catggacatg acctggatta cgatcggcgc
       61 tcaacagccg gcgttcaaca aaaagggcta gcctctgccg caggccggac aagggtctct
      121 ctctattgat tgacctcctt gtagctgctg ggcaatttgt tcttgcaggt cggctttgtc
      181 ggtttgattg tggcggcggt aaaactgtgc cagaacctgt actgctctca ggtgcgcatc
      241 tttaggctca tttcgtacaa gggacaccgc ttccatataa agtagttctg ctctcttgag
      301 gtcggctcgc tggaattcag tagcctgggc ccagcgctgc agagcaaggg ccgactccgc
      361 ctttccaagt tttttagctt catttagggc ttgtctgaag ctggcatcgc cggcggcgaa
      421 atttttgttc ttaaattgag agttgccctg cccctgtagt ttgaccatct tctcccggca
      481 ccggtcctct gccttaccac cggcactgct ctgggcggaa gcaggcgtca gaccggcgcc
      541 caggctgaca gtcagggcca gtaagagggg tccggtttta gatgccaact tttgcaattt
      601 catggttcta cttcctggtt aagtgtccaa cttatcttgt ttagctaatt ttgtttagct
      661 aattttgtcg ctcttgtttt ttaccccatt ttttctgcaa ttccatgccc actgtcagca
      721 tatctagatc gagggtttct atataagtat gcaactgcgc cagttcagcg tcgagaagaa
      781 gtgcctgctt aacttcctgg gcattgagct ttgtttctag taactcaagc agtctttcct
      841 gatagtgttt cagtgcggtg ctcatattat tttgccgatt ttacctgctg gcgaatttcc
      901 ataaactgtt gcacaaactc gctttcatct tctcgggtgc aaattgtgcc gcccattctc
      961 tccaaaatta tccatttaag agctggacag aggggcacta cttcgccgat tagtgccatg
     1021 acttgaggcg gtacgttgcc gtcatgggtg tcgcggcgaa tgcgccggct atggctaatc
     1081 tcgggctccc accaggaacc cccggagatg tgcagttctt ttaccagttc gagggggtag
     1141 taaaagagaa tttctgcgag ggtgagattg aagttaacca tctggcaata gagattgtgc
     1201 agatcaagca ccagaaaacc atttgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnatat gctggtattt gtgaagtgtg caggcaaggc tgaacttctt gaggctttct
     1381 tcaatctctg gtggtctgac cagactcata ggggaaagga aaaaactatg accaagcagg
     1441 ctttcttgct cactgaactt ggcaagcagg ttctctaccc ataggggtat ggctctatcg
     1501 aaaccggcat caaaagtcca ttccaggcat tccaccaggt tatcttgcaa gagcggcagg
     1561 cttgccttgc ggaagtcttc cgtgggcatg agtgaaagac cgacccgggg cgaagttcta
     1621 tttgtctttt gagtttgttc agacaacttt agcgcagcaa ggatttcttg cctggtttgg
     1681 ggcttaaggc ggccagtctt ttgttgatgc gttccagaag gagcttcagc tcatgctctt
     1741 tgatggccag tcgttctacg tcgaccacaa attctttcgc ttccgcctgg gctgcttcca
     1801 gttctcttct tgagactacg ccttctttgt agagttcttc gaagcttacg gcgcggtctt
     1861 ctttgtcttt cagtttgcgc tgtttggctt ccaattccag agcggcgtct ttatattgaa
     1921 tggctacttc tttctctctt tctttgagag tttctttgcc cagcatttcc acgggcatgg
     1981 tcatgcccag gacggcttgc atgcttgggt cgacgagtaa acttggagtc atgggcggcg
     2041 gcggtggcac aagcatggtg gttctggggc gggaagcgct tttgcccttg gcctgagccg
     2101 gcttttcttt tcctgttgct ttgactgccg ttgcttgagc ctgtgattta gcaggtaccg
     2161 gcttttctgg tttgaccgcc ttgtcctgtt tgctagtttg aggagcatct gttttgacag
     2221 gcggtgcttg tttggccgga gcttccggtt tgaccgactt gtcttgtttg ctggtttgag
     2281 gggcggccag aacttctgcc gccggatagt gggcatttag actgagggca agcaggctta
     2341 caactattaa gtttgactga gataacttca tgaccagaaa cgaaatgtaa aaggggacta
     2401 ccgg
//
LOCUS       DAOQNZ010000584         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26408_length_2402_cov_3.858117, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000584 DAOQNZ010000000
VERSION     DAOQNZ010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26408_length_2402_cov_3.858117"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1136)
                     /locus_tag="PKW73_10830"
     CDS             complement(21..1136)
                     /locus_tag="PKW73_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73826.1"
                     /translation="MTFRLSLLTASLAGLCLALPATACLWDYDTLNQEQSRFPSTIEL
                     ITGKFLRHSPKFYQWRIEDRLQKLAKNPEDIALYDDLGVAYQKTGQHEKALETMSAKD
                     KLRPALYETKANLGTFHILSGDLKQGLPFIDEALKIKPHAHFDRERYQKWLVEYALSK
                     YPDGKLNFPLCKPETGPRFPEFLAVKLSGQPGFLSQEASQKAVQAVLGMMRFADYDNP
                     LLLEVLGDLLIASGMPEDGKQLSARAYLAASYKFKGQPEEKEYRVLAEKALHNQARRG
                     DDEQMPLAALEEEFRKERAEAEAWYRNLEHKESLWIEKGLNPDSEFDKLYHSEPRLSE
                     TIDTFGLTLSGLGLMVIFCSFAFWKAYRRAEKKGSDT"
     gene            1486..>2402
                     /locus_tag="PKW73_10835"
     CDS             1486..>2402
                     /locus_tag="PKW73_10835"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HNA73827.1"
                     /translation="MTEGVKLFNARDYEGAVKKFSSLVQTQPNSADAYYYLGASCHRS
                     GRTSDARRYYDYIMVRFPGSKAAEYAKTAAASLSPAGASTAEKSDKKESAAKGSASGT
                     KDKADEIGELQGPDEDRVPLTKGHGGHMFVPVFINGHRLEMFYDTGAESTCVSLTAWT
                     RAGNKAPVRAPDSLVMGTGGLDGSWYEPVTFGMGKFSRTMNISIIKSMPEGIDGLVGQ
                     NFFKDLQYNLTGSANYIHLLRKNSRTASTSVPYNTIDVPFARAGNNMVVIVNVNGEPI
                     EMIFDTGCGTTSISRFQASAIGLRATDGQ"
BASE COUNT          537 a          569 c          654 g          642 t
ORIGIN      
        1 ggcggggggc ggttgttgaa tcaggtatcg ctccctttct tctcggcgcg gcgataggct
       61 ttccagaatg caaaagagca gaagattacc ataagcccta agccggagag agttagcccg
      121 aaagtgtcaa tggtttctga aagccgaggt tcactatggt agagcttgtc gaactcgctg
      181 tctgggttta agcctttttc aatccagagg ctttctttgt gttctaaatt tctataccag
      241 gcttctgcct cggctctctc tttgcgaaat tcttcttcca aggcggcaag cggcatttgt
      301 tcgtcgtcac cgcgtctggc ctggttgtgc aaagcctttt cagccagaac cctgtattcc
      361 ttttcctcgg gctggccctt gaacttgtac gaagctgcta aataggctct ggcactcagt
      421 tgtttaccat cctccggcat gccggaggcg atcagcaagt ctcccagtac ctctagcaaa
      481 aggggattgt catagtctgc aaagcgcatc attcctagaa cggcctgcac ggctttttgc
      541 gaagcttcct gggagagaaa gccgggttgt cctgataatt tgaccgccag gaattctgga
      601 aatctaggac cggtctcggg tttgcaaagc gggaagttta gcttgccgtc agggtatttg
      661 ctaagagcgt attcaaccag ccacttttga tagcgttcac gatcaaagtg ggcatgaggt
      721 ttgattttca gtgcctcgtc tataaatggc agcccttgtt ttaggtcgcc cgaaagaata
      781 tggaaggtgc ccaggtttgc ctttgtctca tatagggccg gtctgagctt gtctttggct
      841 gacatggtct caagagcctt ttcatgttga ccggttttct gataggccac cccaagatca
      901 tcgtagagtg caatatcttc tgggtttttg gccagtttct gcaaacgatc ttctatgcgc
      961 cattgataaa atttgggcga gtggcgcaaa aactttccgg tgattaactc tatggtgctg
     1021 ggaaagcgcg actgttcctg attgagtgta tcgtaatccc agaggcaggc tgtagccggc
     1081 aaggccaggc acagaccggc aagcgatgcg gtgagtaggg aaagccgaaa agtcatgcaa
     1141 atattcccag tctgaagttc ttgagtgatg acactaggga gattttaagg caaggtggct
     1201 gatttagctt cgctgctaac tcagggttta ccctggagtt ttgatgccaa ggtttatgga
     1261 tggaggtttc ggcgcagcct gtcgtctcct aatcgttaat gtgcgtggag atgccgtttg
     1321 ttttgcctgc aaaggtgtaa aaataaggat gggtttgacg gttaaacgag gaacgtttct
     1381 ttgaaggagt gctggcaaat tatctatgca agtcgtagga cagggcaatt tttattgctc
     1441 atcggctgcc tcttgtttgt accagtagct gtgcgggctc agggcttgac tgaaggcgtg
     1501 aagcttttca atgctcgtga ctatgagggt gcagtcaaga aattttcttc ccttgtccag
     1561 acccagccca attccgctga tgcttattat tacctcgggg caagctgcca tcgcagcggg
     1621 cgcacttctg atgcacgccg ctattacgac tacattatgg tccgctttcc cggctcaaag
     1681 gccgctgaat atgccaagac tgccgcggcc tcattatctc ctgcgggggc gagcactgcc
     1741 gagaagtccg ataagaagga gtctgccgca aagggctccg catcgggtac gaaagacaag
     1801 gcggatgaaa taggcgaact tcaaggtccc gatgaagaca gggtgccgct taccaaaggc
     1861 cacggcgggc atatgtttgt gccggttttt attaatggac accggcttga aatgttctat
     1921 gacaccggag cggagtccac ctgcgtttca ctgactgcct ggacgcgggc cggcaataag
     1981 gcaccagttc gcgcgccaga cagtttggtc atgggtaccg gcggtcttga cggctcttgg
     2041 tatgaaccgg ttactttcgg catgggtaag ttttctcgta ccatgaacat ttcgattatc
     2101 aagagtatgc ctgagggcat tgacggcctg gtgggtcaga actttttcaa agacctgcaa
     2161 tacaatctca ccggatccgc caactacatt cacctgctgc gtaagaacag ccgtacggca
     2221 tcgacttctg ttccctacaa tacaattgat gtgcccttcg ccagggctgg aaacaacatg
     2281 gtggtcatag tgaatgtaaa cggcgagcca attgagatga tttttgatac cggttgcggc
     2341 accacttcga tatcgagatt tcaggcttcg gctatcggtc ttcgcgccac tgacggacaa
     2401 ta
//
LOCUS       DAOQNZ010000585         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26485_length_2398_cov_4.274434, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000585 DAOQNZ010000000
VERSION     DAOQNZ010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26485_length_2398_cov_4.274434"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PKW73_10840"
     CDS             complement(<1..747)
                     /locus_tag="PKW73_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73828.1"
                     /translation="MQQSPNNEPSQNNTVKSEPGNQTPVKADTTLEDTTPRPALPKDR
                     PFSPHYFHQTALFVGKQSPRIYITPTAYRKMLLYVELAKLEVGWLGTASKLKGGNFLI
                     EDTFLLHQEVSAAQTELSVEGQSKLAEELLLKGEEGLETINSMRFWGHSHVRMGTSPS
                     GTDDQTMLRFRDDGLEWYIRGIFNKLGRAEFTVYYFDLGFAVKDVPWDVYDPESNTII
                     TPKPLVERGGSLFGSWHERTQARDFCTLAEV"
     gene            complement(885..1769)
                     /locus_tag="PKW73_10845"
     CDS             complement(885..1769)
                     /locus_tag="PKW73_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73829.1"
                     /translation="MNSFIMNSFILINADQFTLDTLAEAGIDYYNWDENSIIVTESQE
                     RLSQALGFKGLNAAASEWDGFYLVDLNEQICCTPTAMPATDSMQGKYANTQLGQAREN
                     YIKLARTRMDQWLAQTLQAFEKTNAKLLSLAGAFFKRAQLAQAKTIKTAGADGSKLLA
                     EQYRALMAIDKVKSVRFSAGRILIFTDVLTATAPNVKGTRELGRFLITVDTTGTLDAP
                     ITCHNLTRRIKAWRSGMHAPQVFADGKLCPAEILESIFQLTAEQDLPTAIELLLQHLQ
                     VCQNDTIGEQLKAWPLVH"
     gene            complement(1773..>2398)
                     /locus_tag="PKW73_10850"
     CDS             complement(1773..>2398)
                     /locus_tag="PKW73_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73830.1"
                     /translation="EDYLAGLYGKSLNAHYQHLGNSVSGQSQAALDKSTEAAFGDFAR
                     QLRQTVAAQKEMLKIEQAPYLDLGAEFDSLVSISKVVGVRVMEDRLIVNTTDLFCTDP
                     RTKIVHWIGPFEITISTNKITWLNKAEHAQSLKMNAPHVNSSGEACMGNMKDVFSTLI
                     TQRQFAACVEAAIAFVEAVNVDDTWGKNINNWPVANSAMLAQNQARG"
BASE COUNT          458 a          574 c          702 g          664 t
ORIGIN      
        1 cacttcagcc agtgtgcaga agtctcttgc ctgggtgcgt tcatgccagc tgccgaagag
       61 ggaaccgcct ctttctacca ggggtttggg agtgatgatg gtgttgcttt ccgggtcgta
      121 cacatcccag ggaacgtctt taacggcaaa gccaaggtcg aagtaataaa ccgtgaattc
      181 ggcccggcca agcttgttga aaatgcctcg aatgtaccat tccagtccat cgtcgcggaa
      241 gcgcagcatg gtctggtcgt cggtaccgga aggagaggta cccatgcgca cgtgggaatg
      301 accccagaag cgcatactgt tgattgtttc cagcccctct tcacctttga gcaaaagctc
      361 ctccgccagt ttgctctgcc cttctacgga cagctcggtt tgagcggcac tgacctcctg
      421 gtgcagcagg aaggtatctt caatgaggaa gtttcctcct ttcagtttgg atgccgtccc
      481 cagccagccg acttccagct tagccagttc cacatagagc agcatcttgc gataggcagt
      541 gggagtaatg tagatacggg gcgactgttt acctacgaat agagccgtct ggtgaaagta
      601 gtgagggctg aagggacgat ctttgggaag agccgggcgt ggagttgtat cttccagcgt
      661 ggtatcggct ttcacagggg tttgattgcc cggttccgat ttgacggtgt tgttttgact
      721 tggctcattg tttggggatt gttgcatttg tgtccctcgg gtagttgctt cgaattggaa
      781 gtgagttggt attgccgaca tgccccgctt cagcctgcgc tgaaggggga tggcggatgt
      841 atttgtctgt ctgtctgtct gtctgtctgc ctttttgccg gccgtcagtg caccagcggc
      901 caggctttta gttgctcacc aatggtgtcg ttttgacaga cctgcaagtg ttgcagtaag
      961 agttcgatgg ccgtgggcag atcttgttct gccgtcaatt ggaatatgga ttccagaatt
     1021 tcagccggac agagcttgcc gtcggcgaag acttgaggag catgcatgcc gctgcgccag
     1081 gcttttatgc gtctggtcag attatggcag gtgatcggcg catcaagagt gcctgtggtg
     1141 tcaactgtga tcaggaaacg ccctaactct cttgtgcctt tgacattagg ggcggtggcg
     1201 gtgaggacgt cggtgaaaat gagaattcta ccggcgctga agcgtacgct tttaaccttg
     1261 tcgatagcca ttaaagcccg gtactgctcg gccagtagtt tgctgccgtc tgctcctgcc
     1321 gtcttgatgg ttttggcttg tgccagttgt gccctcttga aaaaggcgcc ggccagactc
     1381 aacaatttgg cattggtttt ttcaaaggct tgcagggttt gtgccagcca ctggtccatt
     1441 cttgtgcgag ccagtttgat gtagttttct ctggcctgac cgagctgggt attggcgtat
     1501 ttgccctgca tggagtcggt agccggcatt gccgtgggcg tgcagcagat ttgctcgttc
     1561 aggtcgacga gataaaagcc gtcccattca ctagctgcgg cattgagtcc tttgaagccc
     1621 agggcttgag agaggcgttc ctggctttcg gtgacgatta tggaattttc gtcccagttg
     1681 tagtagtcga tgcctgcctc tgccagtgta tccagggtga attggtcggc attgatcaga
     1741 atgaagctat tcattatgaa gctattcatt gcctaccccc ttgcctggtt ttgagccaac
     1801 atcgcactat tggcgacggg ccagttgttg atatttttgc cccaggtgtc gtctacattg
     1861 actgcttcta cgaaggcaat ggctgcctca acacaggctg caaactgcct ttgggtgatg
     1921 agagtgctga agacgtcttt catattaccc atgcaagctt ccccggaaga attgacatgg
     1981 ggggcgttca tcttcaagct ttgcgcgtgt tccgccttat tcagccaggt aattttgttg
     2041 gtggaaatag tgatctcgaa aggtccgatc cagtgcacga ttttggtgcg aggatcggtg
     2101 cagaagaggt cggtggtgtt gactatcagc ctgtcttcca tgactctcac gcctacgacc
     2161 ttggagatgc tcaccaggga gtcgaattcg gcgcccaggt caaggtaagg tgcttgttca
     2221 atttttagca tttccttttg agcggcgacg gtttgtctca gttgtctggc gaagtctccg
     2281 aaggccgcct cggtggattt gtcgagagcg gcctggcttt gcccgctgac gctgtttccc
     2341 agatgttgat agtgagcatt gagagacttg ccgtagagac cggcaaggta gtcttcgc
//
LOCUS       DAOQNZ010000586         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26559_length_2395_cov_2.810684, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000586 DAOQNZ010000000
VERSION     DAOQNZ010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26559_length_2395_cov_2.810684"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PKW73_10855"
     CDS             <1..1287
                     /locus_tag="PKW73_10855"
                     /inference="COORDINATES: protein motif:HMM:NF017541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HNA73831.1"
                     /translation="AMVAGLASADGRAAALAALPEVARTGTHLFTFVEYVQSFRGWGR
                     GLRRAIARWYEVQPPEKLAVQLTKYQQRNGWSHRDLLRLAHPKAEDAVRNGLYRFAVT
                     GSTEGLEDSTVLAFQKLRQCENLDGAATAKIVEEARLSMEMVPAELRYPEVYRVLAKQ
                     AGLEWLLRNLGNLSRVAVLSAGDRKLLETVCQRLNNQDGLDKARLHPLKILAALAVYR
                     SGSGVRGSGRWNPETAVVDALDEAFYKAFKYVTPTGKRLCLGLDVSGSMAGTTVNGIA
                     GLTAREACGAMALVTAKSERPSNVSFVAFDTKPYHLALSANQRLDDVVNILSRTGGGG
                     TDCAIPIQWARKKKVPVDVFVIYTDSETWAGDEHPLQALNAYGEAMGIDAKLVTVAMA
                     ANRISLADCTDKRMLNVVGFDTVTPELISQFVMGDL"
     gene            complement(1512..>2395)
                     /locus_tag="PKW73_10860"
     CDS             complement(1512..>2395)
                     /locus_tag="PKW73_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73832.1"
                     /translation="YTCPCPSVNLSRLPAAASFEQDGFRFQLKDSDVFIDQKVKDNWQ
                     RLAVVAYGGLGQTAYIDDLNGDGRKDLLLASSTASCAPLPQTRVQVILTREKGTVLFD
                     QIGFARAGTVFSDRLRLPGLSRNPVLVMEQLHGDGKESYWSTELLAFQPDGMVPLKKF
                     QGSNLPIRISYSMKPNRRILPAEKLSKLGEKSVETVTTLKAINRDAQSMPESFLFRNG
                     ALIEAAPSIRAGQPQACLYLERGGQRSAVSLDTVYGGEILTEAKERNLPLVLTLGSLS
                     GQIKSIKLSIPEPDRLN"
BASE COUNT          556 a          628 c          662 g          549 t
ORIGIN      
        1 gctatggtgg caggtctggc cagtgccgat ggtagagcgg cggctctggc agcattgccg
       61 gaggtggcac gcaccggtac gcaccttttc acatttgtgg aatatgtgca gtcctttcgc
      121 ggctggggca gaggtctcag gcgagcgatt gcacgctggt acgaagtgca gccacctgaa
      181 aaactagcgg tgcagttgac taaatatcaa cagcgcaatg gctggtcaca tcgtgatttg
      241 ctgcgccttg ctcacccgaa ggcagaggat gccgtgcgca atggtctcta tcgttttgcc
      301 gtaaccggct ctaccgaagg gttggaagat tcgaccgtgc tcgccttcca gaagttgcgc
      361 cagtgcgaaa accttgatgg agcagctacc gctaaaattg tagaggaagc caggctttcc
      421 atggaaatgg tgcctgccga attgcgctat ccggaagtct accgggtact ggcaaagcaa
      481 gccggactgg aatggctttt gcgcaatctt ggcaatttgt cgcgggttgc cgtgctgtcc
      541 gccggcgatc gaaaactgct tgaaacagtc tgccagagac tgaacaatca ggacggtctc
      601 gacaaagcca ggctgcaccc gctcaaaatt ctcgccgccc ttgcggtcta ccgctcgggt
      661 agcggagtac gcggcagcgg caggtggaac cctgagacag cagtcgtgga tgcacttgac
      721 gaggctttct acaaggcttt caagtatgtc acgcccacag gcaagcgact ttgtctgggt
      781 ctcgatgttt ccggttccat ggccggcacc acagtcaatg gcatagccgg tctcactgca
      841 cgtgaggctt gcggagccat ggctctggtg acagccaaaa gtgagcgacc ttccaatgtc
      901 tctttcgtgg ccttcgatac caaaccttat catctagcgc tcagtgcaaa ccagagactt
      961 gatgatgtgg tgaatatctt gagccgcacc ggcggcggtg gtacagactg tgccataccg
     1021 atccaatggg cgagaaagaa gaaggtaccg gtcgatgttt ttgtcattta caccgactcc
     1081 gaaacatggg ctggagacga gcatccgctg caagcactaa atgcctatgg tgaagccatg
     1141 ggtattgatg ccaaactggt aacagtagca atggcggcca accgcatctc gcttgccgac
     1201 tgcaccgaca agcgcatgct aaatgttgtc ggtttcgaca cagtcacccc agaactaatt
     1261 tcgcaatttg tcatgggtga tctataatag aggatgtcgg gtcgaaggct caggttatcg
     1321 acttgtaatc gagaggtcgc gggttcgact cccgtcgcct gttttggaaa caaagcaagc
     1381 gtagctcagt ggtagagcac tatgcctggt ccgctactta tccgacatcc tctttatttg
     1441 accgggacgg agcccgaggg ttatcactga tactgatccc ctccctctgg cagttaattt
     1501 gaccggtctt actagttaag tcgatcaggc tcaggaatac tgagcttgat cgactttatt
     1561 tgtccagata aggagccgag agtgaggacg agaggcaaat ttctctcctt ggcctcagtc
     1621 aatatctcgc caccataaac ggtatcaagg ctaacggcgc tccgctgccc gccccgttca
     1681 agataaaggc aagcctgagg ctgccccgct ctaatagacg gcgccgcctc aatcaaggcg
     1741 ccgtttcgaa agaggaaact ctccggcatg gattgtgcat ctctattgat agctttcagg
     1801 gtagtgacag tttctaccga tttctcaccc agcttgctta gcttctcggc aggcaaaatt
     1861 ctccggttgg gcttcatact gtaacttata cggataggca aatttgagcc ctgaaacttt
     1921 ttgaggggca ccatgccgtc cggctggaag gcaagaagtt cggtggacca gtaactttcc
     1981 ttgccgtctc catgcaactg ttccatcaca agtactgggt tacggctcag accgggcaat
     2041 ctgaggcgat cgctgaagac agtgccggcc ctggcaaagc caatttgatc aaagagtacc
     2101 gttccttttt cccgagtcaa aatcacctgc accctggtct ggggaagcgg cgcacaagag
     2161 gccgtggatg aagccaaaag caaatctttc ctgccgtcgc cgtttagatc gtctatgtag
     2221 gcagtctgcc ccaagccgcc ataggcaacc acggcaaggc gctgccagtt atcttttacc
     2281 ttttggtcga taaagacatc ggaatcttta agctgaaagc gaaagccgtc ttgctcaaag
     2341 gaagcagcgg cgggtagtcg gctaagattg acggaaggac aggggcaggt atacc
//
LOCUS       DAOQNZ010000587         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26582_length_2394_cov_3.340316, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000587 DAOQNZ010000000
VERSION     DAOQNZ010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26582_length_2394_cov_3.340316"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PKW73_10865"
     CDS             complement(<1..1081)
                     /locus_tag="PKW73_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73833.1"
                     /translation="MNIYGLKSKNKPLSVKLSQFFALSLTASVIAGFSSTSFSTTAAR
                     AQSSAVSRAQKYRNDGNTALEAGKYAKAITAFGSALKQMDQDGQNSSPEYLSTLIGLA
                     RAHKESDQLDAASQRINEAANLARKLGNGAPELQATFDSINALCKQVDIDTLGTGTTN
                     ALKSNSSTMTVVKDSSGYKVTIRTPAKFESSTGNDKVDMLGLDPVVTFSLAQDSDGTV
                     RVYDVKGMRMHSVEKAMWVNFFGCRFGPQNEQSYRPSTISAGKMGVVKEVSRDLPPRI
                     FAPVAGILAAISTMGQPVDPPLPAYTAPIEAVSKTEPASQNATTTSPPTSQTEAITKS
                     QPIGQSETRPASTPVVQSETPPASTP"
     gene            1362..2222
                     /locus_tag="PKW73_10870"
     CDS             1362..2222
                     /locus_tag="PKW73_10870"
                     /inference="COORDINATES: protein motif:HMM:NF008913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HNA73834.1"
                     /translation="MRHPDVPESLDGWWILHRMFAFDRLAWDSVPDDERKEIVQEARE
                     ALEALKGTEDSDVGLAQMLGHRADLMLTHYSRNYEGLAHAQTVVDKLRLTEFLEPTTS
                     YVSILELGLYDATGKIHAALAQQELKPGTQEWIQAFDQMVKEQEKVPHNAGRLWARIP
                     QRRYVCFYPMDKKRGEDVNWYMLPFADRARLMLDHGKIGRTYHGLVTQVISGSIGFDS
                     YEWGVDLYADDPIVFKKLIYEMRFDEASAKYGHFGDFYSGVQFSLDQLPTYLDGNSVP
                     ALNVLETAKA"
BASE COUNT          470 a          614 c          688 g          622 t
ORIGIN      
        1 ccggggtaga tgctggtggt gtctcagact gaacaaccgg agtagatgct ggtcgtgtct
       61 ctgattgccc aattggctgc gattttgtaa tggcttcggt ttgacttgtc ggtgggcttg
      121 tggtggttgc attctggctt gccggctcag ttttgctgac cgcttcaatg ggagcggtgt
      181 aggcaggcag aggcggatcc accggctgcc ccatggtact gatggcagcg agaatgcctg
      241 ccaccggtgc gaaaatgcgg ggcggcaggt cgcggctgac ctctttcact acccccatct
      301 tgccggcgct gatagtggaa gggcgatagg actgttcgtt ttgcggtccg aagcggcagc
      361 caaagaagtt gacccacatt gctttctcga ctgagtgcat gcgcatgcct ttgacgtcgt
      421 agacgcgcac cgtgccatca gaatcctgcg ccagagagaa ggtgactacg ggatcgagcc
      481 cgagcatgtc tactttgtcg ttgccggtgg aactctcgaa ttttgcggga gtacgaatgg
      541 ttaccttgta gccgcttgag tctttcacca cggtcatagt ggaagaattg cttttgaggg
      601 catttgtggt gccggtgccg agggtgtcga tgtcgacttg tttgcagagt gcattgatgg
      661 agtcgaaggt cgcttgcaac tccggggcgc cgttgcccag tttgcgagcc aggtttgcgg
      721 cttcgtttat acgctgactg gctgcgtcca gctggtctga ctctttgtgc gccctggcca
      781 gaccgataag ggtagaaagg tactcagggc ttgaattctg accatcctgg tccatttgct
      841 tgagggcgct accgaaggcc gtgatggctt tggcgtattt gccagcttca agggctgtat
      901 tgccgtcatt tctgtacttc tgagcgcggc tgaccgcact gctctgagcc cgggctgccg
      961 tggtcgagaa agatgtgctt gaaaaccctg caattaccga cgctgtgagg ctgagggcga
     1021 aaaactggga aagtttaacc gatagcggtt tgtttttgga ttttaagccg tagatattca
     1081 tttaagcctt tcccgtattc cttgccagta ctgcatttta gaccttgatg ccgggctcgc
     1141 acgcagattt tagtgccagc gctaatgatt atagagggca tgcaagggct gccgctgctc
     1201 cttatccaag aaataggcgg ctgccccgat aacgacggag cggttggggt gtcaagagcg
     1261 gcagtgttgc gctcgaatac aaagacttgc ttagtttcct cacccttgcc aagtaattgc
     1321 cttaggctta agtcgttctc gttgttttaa ggagctattt catgcgccat cccgacgtac
     1381 cggaatcttt ggacggttgg tggattctcc accgcatgtt cgccttcgac cgccttgcct
     1441 gggactctgt gccggatgat gagcgtaaag agatagtcca ggaagccaga gaggcgctgg
     1501 aagccctcaa gggcaccgaa gattcggatg tcggcctggc tcagatgctc ggtcaccgcg
     1561 ctgacttgat gctcacccat tacagccgta actatgaagg gctggctcat gcccagaccg
     1621 ttgtcgataa actgcgtttg accgagtttc tcgaacccac cacctcctat gtttccatac
     1681 tggaacttgg tctctatgat gccaccggca aaattcacgc cgcccttgcc cagcaggaat
     1741 tgaagcccgg tacccaggag tggattcagg ctttcgatca gatggtcaaa gagcaagaga
     1801 aagtgcccca caacgccggt cgcctctggg cgcgcatccc gcagcgccgc tatgtttgct
     1861 tctatcccat ggacaagaag cgcggtgaag acgttaactg gtacatgttg ccctttgccg
     1921 accgggcccg cttgatgctc gatcacggca agattggtcg cacctatcat ggtctggtaa
     1981 cccaggtgat ttccggctct atcggctttg acagctatga gtggggcgtc gatctctatg
     2041 ccgacgatcc gattgtgttc aaaaagctga tctatgaaat gcgttttgat gaggccagcg
     2101 ccaagtacgg tcacttcggt gatttctatt ccggcgtgca gttctctctc gatcagctgc
     2161 ctacctatct agacggcaat tctgtgccgg ctttgaacgt gctggaaacg gccaaggctt
     2221 agttcgggca cagtctttga ctgtgcttct ggactgtgct tctgaaaact taagagtgca
     2281 agaaatttag ctaaatttct tgcactcttc cttatctgcc tgagtaatgc cggcatccta
     2341 atttagctgc taatggccag ttgctgcagg gtgtccccca gttccgccac ggtc
//
LOCUS       DAOQNZ010000588         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26604_length_2393_cov_3.822070, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000588 DAOQNZ010000000
VERSION     DAOQNZ010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26604_length_2393_cov_3.822070"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2163
                     /gene="ovoA"
                     /locus_tag="PKW73_10875"
     CDS             <1..2163
                     /gene="ovoA"
                     /locus_tag="PKW73_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HNA73835.1"
                     /translation="VLSYFDNGWTLSELLFSALNGEEGFYRPPYHELRHPLVFYYVHP
                     AALYVNKLRVAGLIENGINSYFESLFETGVDEMSWDDMAKNTIAWPAIDEAHRYRKQV
                     YELVKNLILNHPDLAAPGSVNMEHPLWALLMGFEHERIHLETSSVLMRELPLHLLQKP
                     QAWPEKAPIARLEDTAFPPKVGRHYPQNALVEIARSQLNFGKSNHWPTFGWDNEYGSR
                     QVDLPAFAATSRLISNGEFWQFVAEGGYLNSEYWSETGSRWRQFRNIKCPTFWVPDGP
                     AGTHQYKLRTCFEIIPMQWNWPAVVNYHEAQAFCRWKSEKDGKPYNLPSEAEHQAMRA
                     AVLSTAAESNNLLRFGSECATDKFPSPLKEGTAAIYDLFGNLWQWLEDHFHPLPGARV
                     HRYYDDFSTPCYDGQHQMIMGGSFISTGDEADIYARFHFRPHFFQHAGFRLVVRPEGS
                     DGAVVLLKENAADANPYEAQTTLNEYMNLHYGKVEDQMPWAMGPKEAACFPQRCAELV
                     DRFCRETAVTRKRALDIGCAVGGASFHLARTFESVVGIDLSKSFIEAARTLQSEHKLA
                     YDLKLEGDLQERRLAEVDPELSRRVTFRQADACALPADLLDFDAVLLANLLCRLPSPA
                     ACLGRLDGDRGLVKPGGIVVVVSPYTWMERFTPREVWLGGYQDDEGREHYSEEGLQAA
                     LGKNFDLLDKHDMPLLIREHERKYQYIVSQAMVFRRRL"
BASE COUNT          562 a          709 c          621 g          501 t
ORIGIN      
        1 gtgctctcat attttgataa cggctggact ctctccgagc ttttattcag cgccctgaac
       61 ggcgaagaag ggttttatcg cccgccttat cacgaactga ggcatccgct cgtcttttat
      121 tacgtgcatc cggccgctct ctacgtcaac aaattaagag tagccggctt aattgaaaac
      181 ggcatcaact cttattttga aagcttgttc gaaacaggcg tcgacgaaat gagctgggac
      241 gacatggcca aaaacaccat cgcctggcct gccatagacg aagcacaccg ctatcgcaaa
      301 caagtctacg agctggttaa gaacttgatt ttgaatcatc ccgacttagc cgcaccaggg
      361 tccgtgaaca tggaacatcc actctgggcg cttttaatgg gctttgagca tgagcgcatc
      421 cacctggaga cctcgtcggt cttgatgcgc gaactgcccc tgcatctgct gcaaaaaccg
      481 caagcctggc cggaaaaggc accaatcgcc cgcctggagg acaccgcatt cccgcccaag
      541 gttggtcgcc attatccgca aaatgccctg gtggaaatcg ccagaagcca acttaacttc
      601 ggcaaaagta atcactggcc gacctttggc tgggataacg aatacggcag tcgccaggta
      661 gacttacccg ccttcgccgc caccagtcgc ttaatttcca acggtgagtt ctggcagttt
      721 gtggccgaag gcggttatct gaacagcgag tactggagcg aaaccggctc tcgctggcgc
      781 cagttccgaa acataaaatg ccccactttc tgggtaccgg atggaccggc cggcactcac
      841 cagtacaagc tgcgcacctg cttcgagata atacccatgc agtggaactg gcccgccgtg
      901 gtcaactacc acgaagccca ggccttctgc cgctggaaat cggaaaaaga cggtaaaccc
      961 tacaaccttc ccagcgaagc cgaacaccag gccatgcgtg ccgcagtgct aagcaccgca
     1021 gccgaaagca ataatttact gcgcttcggc tcggaatgtg ccaccgataa attcccaagc
     1081 ccgctcaaag agggcactgc cgccatctat gacctattcg gcaatctctg gcaatggctc
     1141 gaggatcatt tccatcccct gccaggcgcc agggtgcaca ggtattacga cgacttcagc
     1201 acaccttgct acgacggtca gcaccagatg atcatgggcg gctccttcat aagtacaggc
     1261 gacgaagccg acatctatgc ccgctttcat ttccgtccgc acttcttcca gcatgccggc
     1321 ttccgcctgg tagtcagacc agagggcagc gacggagccg tggtgctcct gaaagaaaac
     1381 gccgccgatg ctaatcccta cgaagcgcag accaccctca acgagtacat gaacttgcat
     1441 tatggaaaag ttgaagatca aatgccctgg gccatgggcc cgaaagaagc agcctgcttt
     1501 ccccaacgct gcgccgaact ggtggaccgc ttctgccggg aaactgccgt gaccagaaag
     1561 cgtgccctcg acataggctg cgccgtgggc ggcgccagtt tccacctggc cagaaccttc
     1621 gaaagcgtag tgggcattga cttgagtaag agcttcattg aagcggcacg caccttacag
     1681 agcgagcaca agctcgccta tgatctgaag ttggagggcg acttgcagga gcgccgcctg
     1741 gccgaggtag atccggagtt gtccaggcga gtcaccttcc ggcaagccga tgcctgcgct
     1801 ctgccggccg accttttaga tttcgatgcc gttctgctgg ccaatctact ttgccggctg
     1861 cccagcccgg ctgcctgcct ggggcgcctt gacggcgacc gcggtctggt caagccggga
     1921 ggcattgttg tggtggtttc cccttacacc tggatggagc gcttcacacc aagagaggtc
     1981 tggttgggcg gctaccagga tgatgaaggt cgcgaacatt attcggagga agggctgcaa
     2041 gccgcactgg gcaaaaattt cgacctactg gacaagcatg acatgcccct cctgatcaga
     2101 gaacatgagc gcaaatatca gtacatagtg tcccaggcca tggttttccg acgccgcctc
     2161 taaatcacag ccgcgcaaca tcgcatctgc tttgcttaac aggaaaagat acagggttaa
     2221 ccacctgtat cttttatcaa tgcgggggga atatatccga aaagacccgg gtttatcatg
     2281 agttccgatc aaagcaacac cacgctgaca attgcctatt ccgacagatt ttccaccctc
     2341 atgctctggt ggaaggcgat ttttctggtg cccatctggg gtctcctcct ctt
//
LOCUS       DAOQNZ010000589         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26680_length_2391_cov_3.553510, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000589 DAOQNZ010000000
VERSION     DAOQNZ010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26680_length_2391_cov_3.553510"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..824)
                     /gene="trmD"
                     /locus_tag="PKW73_10880"
     CDS             complement(78..824)
                     /gene="trmD"
                     /locus_tag="PKW73_10880"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNA73836.1"
                     /translation="MPEITFHVITLFPELIDSYCRTSIIGRGAKAGKIACRSYNPREY
                     CQDKYRKVDDTPYGGGAGMVMKPEPIFACLEAIERKPDSPVIITSPQGKPFQQSDAEE
                     MAKMTDITIICGHYEGFDERIHSLATHEISLGDFVITGGELAALVIVDAVGRLIPGVL
                     GQSLSLHQESFNEGNDGLLEGPQYTKPPVFRDMEVPEILRNGDHKKINRWRREQALKR
                     TLERRPDLLNKAKLNAQDLNFLKKLQEGLQ"
     gene            1014..1838
                     /locus_tag="PKW73_10885"
     CDS             1014..1838
                     /locus_tag="PKW73_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73837.1"
                     /translation="MVSGSEVRRQSKGGLSRSETRKFVENHPASQTIMPRNIDRSMFG
                     HAEPEVGRSLNKGEHVAHMELSEQFISGFGEERSVDVMAWMDELKADSSSFIASRFDE
                     LVQEEARMRRYRTAVSSLIDKIFQDLRYFAFEFNKVARGTSMQMVATALGEVTEVIQV
                     NSKREVEASATYFRARLANRHYALVLRGAERRIEFYLVPVAQVMGQSTVESQYLPMAS
                     LEIRLRGEELSWRLAGEESVNLPTGSIEELSMYLFGTLVDVTKQEVRRIEAEGGVC"
     gene            2078..>2391
                     /locus_tag="PKW73_10890"
     CDS             2078..>2391
                     /locus_tag="PKW73_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73838.1"
                     /translation="MRRLCAVLALLLNLGVALPYCGWCSRALAQGPYIQSAERLESEK
                     NFAAAARAYGEAIKIVGRDKQVDKAKLGPLYFKRGYCYQRSGASEEAVLDLERSVSLG
                     YRG"
BASE COUNT          495 a          552 c          706 g          638 t
ORIGIN      
        1 atagcacctg ctaaaggtgg ctctgcgtaa ggcatttgca attgagcgcg gcgcaaaaaa
       61 ggtttttcag ccaagcccta ttgtaaacct tcctgcagtt ttttcaagaa gtttagatct
      121 tgtgcgttca acttggcctt gttgagaagg tccggtcttc tctccagggt gcgcttcagt
      181 gcttgttctc gtcgccagcg gtttattttt ttgtggtcac cgttgcgcaa tatttcgggt
      241 acttccatgt ctctgaagac aggcggttta gtatattgcg gaccttccag caagccgtcg
      301 ttgccctcat tgaaggattc ttgatggagc gaaaggctct gcccgagcac tcccggtatc
      361 aatctgccga cggcatcgac tattacgagg gcggcaagtt caccgccggt gatgacaaag
      421 tcgccaaggg aaatttcgtg agtggccagg ctgtgtattc gctcatcgaa gccttcgtag
      481 tgtccgcaaa tgatggtgat atcagtcatc ttggccattt cttcagcatc gctttgttga
      541 aagggcttac cctgggggct ggtaatgatg acgggagaat ctggcttgcg ttcgatagcc
      601 tccaggcagg cgaagattgg ctccggtttc atcaccatgc cggcgccgcc accataaggg
      661 gtgtcatcaa ccttacggta cttgtcctgg caatattctc tggggttata gctgcggcag
      721 gcgatttttc ccgctttagc gcccctgcct atgatgctgg tgcggcagta actatcgata
      781 agttccggga agagcgtgat gacgtggaag gttatttctg gcattgatcc ttagtgtttg
      841 cttgcaggtg atcccaacac taaacatggt tttcaacctg gggcttatcc gtaacctgat
      901 tttcccatag cttggcaaaa tatacgcgct ttcgttaata cggtttggtc gatgacagcc
      961 gagcggcatt tttgcacaat agaaaggcga gcagcagttt tgaggtggtc aaaatggtca
     1021 gcggaagtga agtaagacgg cagtctaaag gcggtctctc tcggtctgag actagaaagt
     1081 ttgtggagaa ccaccccgct tcgcagacga ttatgccgcg caatattgac cgttcgatgt
     1141 ttgggcatgc tgaaccggaa gtcggccgca gcctcaataa gggtgagcat gtggctcata
     1201 tggagctatc cgagcagttc atcagcggct tcggcgagga gcgttcagtt gatgtaatgg
     1261 cctggatgga tgagctgaaa gctgattcca gcagttttat cgcttcacgt ttcgatgaat
     1321 tggtgcagga ggaagcgcgc atgcgccgct accgcacggc cgtctcgtct ttgatcgaca
     1381 agatttttca ggatttgcgc tattttgcct ttgaattcaa taaggtggct cgcggcactt
     1441 ccatgcaaat ggtggcaacc gccctgggag aagtgacgga agttattcag gtcaactcca
     1501 aaagggaagt ggaggccagc gccacctatt tccgggctag attggctaac aggcactatg
     1561 cgctggtctt gcggggtgcg gagcgccgca tcgagtttta cctggtgccg gtggcccagg
     1621 taatgggcca gtcgacggtg gaaagccagt acctgccgat ggccagtctg gagattcgct
     1681 tgcggggtga agaactctcc tggcgtttag ctggcgagga gtctgtaaat ctgcccactg
     1741 gcagcattga agagctgtct atgtaccttt tcggtaccct tgtggatgtg accaaacagg
     1801 aagtaaggcg tatagaggct gaaggcggag tctgttagcc tgccctgccg ctgcttgcgc
     1861 ctatgcctta ccggcagtaa attattctgt ctcaagggta gtttcgcctt gccagtttgg
     1921 gatttgccgt tcaaactgag agatagtcgt atggaatgca gcggcagttt accggctggt
     1981 tctttcgatt cttgccggtt catggttcag gcaggcagtg ggtcagcggg ttagccgagt
     2041 cagttagcca attttattgg tgaaggtcga gggatttttg cgccgtttat gtgccgtttt
     2101 ggccttacta ttgaatcttg gcgtcgccct tccgtattgc ggatggtgtt ctcgggccct
     2161 ggcgcagggg ccttatattc agtcggcgga aaggcttgag agcgagaaga acttcgcggc
     2221 ggcagccagg gcttatggcg aagctatcaa aatcgtgggt cgggacaaac aggtcgacaa
     2281 ggctaaactc gggccgcttt atttcaaaag aggatattgc tatcagcgca gtggtgcttc
     2341 ggaagaggct gtcctcgatt tggagcgcag tgtttcgctt ggctatcggg g
//
LOCUS       DAOQNZ010000590         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26686_length_2391_cov_3.196918, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000590 DAOQNZ010000000
VERSION     DAOQNZ010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26686_length_2391_cov_3.196918"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKW73_10895"
     CDS             <1..318
                     /locus_tag="PKW73_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73839.1"
                     /translation="VPIFPLATLGCDEAVPILTNMERTAKFLGLPAFPVTPFFPWLPF
                     PLNLGSLPTKWHMSLIKPLTYEASQDRDELEECAMRQSQFAEGEIQAELNRLLRQQWR
                     HHG"
     gene            complement(436..1185)
                     /locus_tag="PKW73_10900"
     CDS             complement(436..1185)
                     /locus_tag="PKW73_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73840.1"
                     /translation="MTTQNDDGNAHEDKENPRNILPLKVANFLSKMRIAQAQQPKEPD
                     KTPPIPDVNYDLSFAYSFHWEEPGRQIKEALKVLLNRESENQEDTNAVKTARRRAPSQ
                     TIKQPAGGEKQLNTAALEAARIRYLLAINPNTPPPVLEHLTRNAPIPLLERIAENPRT
                     HSTTLARLATHEDFQVRAAVAENLNTSIKTIWKLARDVHADVRFRVAECYCVPLVVLK
                     VLAEDDNPFVAYRAQKTVWRILKEVTELRTA"
     gene            1945..2385
                     /locus_tag="PKW73_10905"
     CDS             1945..2385
                     /locus_tag="PKW73_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73841.1"
                     /translation="MSSDNIWHSVNDPQTEASVLEALVGEAEKHLPLLRRLAEHPRLP
                     AAALQALSEHDDCEVRAAVAANESASLELLSKLIRDSSADVRYCLAESYTLPTNLLAI
                     LLEDENPYVSHRARTTLRRQEVHVVATIPVNRNDSGIDRACGSA"
BASE COUNT          478 a          586 c          692 g          635 t
ORIGIN      
        1 gtgccgatct tccctctggc cacacttggt tgcgacgaag ccgtgccgat tctaaccaat
       61 atggagagaa ccgctaagtt cctcggttta cccgcctttc ccgtgacgcc cttcttcccc
      121 tggttgccct ttcctctcaa cctgggctca ttgcctacaa aatggcacat gagtctgatc
      181 aagcccctca cctacgaggc ttcgcaagat cgggacgaac tggaagagtg tgccatgcgc
      241 cagtcgcaat ttgccgaagg agagattcaa gccgaactga atcgcctgct caggcagcaa
      301 tggcgccatc acggctaatt gtccggtttc tttgaaaaaa cgcagaaact atgccaaaaa
      361 ttagaccgct atcggttcag tttttgccgg ggcaaacagc ttcaccgata gcggtccttg
      421 tttctttcaa acggcttaag ccgtgcgcag ttcggtcact tctttgagaa tgcgccagac
      481 tgttttctgg gctctgtatg cgacaaaggg attgtcgtct tcggccagga ccttcagcac
      541 gaccaggggc acacagtaac actcagctac ccggaagcgc acatcggcgt gcacgtccct
      601 tgccagtttc cagattgttt ttatagaggt gttcaggttc tccgccacgg ctgctcttac
      661 ctggaagtct tcatgagtag ccagccgggc cagggtggtg gagtgtgtgc gtggattctc
      721 ggcaatgcgt tcgagcagag gaataggcgc gttgcgcgtg agatgctcca gcactggtgg
      781 aggggtgttg ggattgatag ccagaagata gcgaattcgg gcagcttcaa gggccgcagt
      841 gttgagctgt ttttcgccgc cggccggttg tttgatggtc tggctcggcg cccgcctcct
      901 ggcagttttt accgcgtttg tatcttcctg gttttcgctt tctcgattca gtaacacttt
      961 tagtgcctcc tttatctgac gacctggttc ttcccaatga aaggaatagg cgaacgagag
     1021 atcgtaatta acgtctggga tgggtggtgt tttatctggt tctttgggtt gttgggcttg
     1081 cgcgatgcgc attttgctaa gaaaattagc gacttttaga ggcaggatgt tgcgtgggtt
     1141 ctccttgtcc tcatgagcat tgccatcatc gttttgtgtg gtcattgcca atacccgaat
     1201 gctcgctcat gaaggttgtg gttcagctct ggaaattcag gtttttccag cctgttttcc
     1261 cttagcgctt gctagcgatg aaaacagcca cgcgttgtgt ccctaattca ttattgaacg
     1321 gggctctgca gccggggttt gggtttggct atatattttt atgggttagg cagggggcgt
     1381 tgcctgcggc tttttcttgc aagccgcaag agaggtcgaa aatggcgtgg cttggggttt
     1441 tcgctccgga ctccgattgt taagagagga ccgagccggc attaaaactg gattccatca
     1501 ggacttgaaa gtaacttttt gcgcaaaggc ggggtcttga ttgcgaaatg ttaacggcgc
     1561 cgagctctgc agaggtgtca ggcgggtgac cggaagcccg gaaagccttc tgtctgagcc
     1621 tgtttagtac tgcctttctg gctgcttctc tggaaaccgg ctttgtgtgc ggctttgggc
     1681 tgggttgttg gttgtacctc ggctgtctga gccgttccct ctcaggaggt aaggggcaac
     1741 catagaagaa gatatgagtt ttccctgata ggcaggcgct ctcaggagta ctatggtagt
     1801 ggtgaaggcc tggcggaaac gggaaaccca gtagcctcgg atcatttaag gaagtcttcg
     1861 gttcgctcct gaatgcagag tggtttcaga ccagagtcag tcaggtttgg agtgggtgca
     1921 aactcccatt gttacggtgt aaatatgagc agtgataaca tatggcattc tgtcaatgat
     1981 ccccagacgg aagcctctgt cctggaggca cttgtagggg aggccgagaa gcatttgccg
     2041 cttctgcggc gcctggctga gcatccacgc ttgccggcgg cagccttgca ggccttgagc
     2101 gagcatgatg actgtgaggt gcgggctgcc gtggctgcca atgaaagcgc ctccctggaa
     2161 cttttgagca aactgattcg ggattccagt gccgatgtgc gttattgtct ggctgaaagc
     2221 tataccctgc ccactaatct tctggccatt ctgctggaag atgaaaaccc ttacgtcagt
     2281 catcgagcca gaaccaccct gcgccggcag gaagtgcatg tggtggccac gattcccgtc
     2341 aatcgtaatg acagtggcat agacagagct tgtggttccg cctgagggca t
//
LOCUS       DAOQNZ010000591         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26817_length_2385_cov_3.881974, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000591 DAOQNZ010000000
VERSION     DAOQNZ010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26817_length_2385_cov_3.881974"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKW73_10910"
     CDS             <1..242
                     /locus_tag="PKW73_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73842.1"
                     /translation="LGLKAPLPSDLAGTIEAEVKTGLSAEGESISIALYQIDSGNCGV
                     VVTSVPRKGGELDLGKNHENVRSILDMLEKSSMAL"
     gene            336..1733
                     /locus_tag="PKW73_10915"
     CDS             336..1733
                     /locus_tag="PKW73_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73843.1"
                     /translation="MLTGILCLAVFFVMAVAMYGRKLSALLALPLMAIAIALIGGIDG
                     AVIVKDVLSAGSVKLHNAYTATMFGAMLAELINKHGMARSLVRFVAEFAGDSPYLLGL
                     LLTGVTALLFSTLGGLGAVIMVGTIVLPVMLSLGIPALAAAGLFLFGISLGGMFNLTN
                     WQLYTDVLKIEQATIISFVVPFSLILGLVILLFLLFELKEKRNAGYFSIGLLVLGALY
                     YPLSRFNPRDASAFAAFSDFSYPFAFFSLLALSAYALFRHFTRQENVPGFSLLTPIVP
                     LIFVLVFRWEIIPAFIMGIAYGALTTWKRDSINILTRSIIDGVTNVIPAVLLMMGIGM
                     LIAAVMDKGVSAAIAPLIKAVVPGSPLTYVLTFTLMAPLALYRGPLSLWGMGSGLVTL
                     IINLTTLTAQAVMGMLMSVGQVQGICDPTNTQNIWIATYLGVDTQVILRKTLPYALLA
                     AFLGLSLAVLKGYVQ"
     gene            1730..>2385
                     /locus_tag="PKW73_10920"
     CDS             1730..>2385
                     /locus_tag="PKW73_10920"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HNA73844.1"
                     /translation="MSNTYRIGIDVGGTFTHAVAIDGLTSNLVGKAKVPTSHSAPEGV
                     ARGIVESLHLLLADTGIAPDTVSFIAHSTTQATNALLEGDVVKVGVLGMSSPALAPLA
                     SAATRLGRIPLAGHGAKGVYMETAHRFLAVESRASGTATGELDASQLEQVEKLLLELK
                     AEGCGAIAVSDAYSVDDGKREEAVLAVCGRLGLLATSGSEVSRLYGLKVRTRTAAINA
                     G"
BASE COUNT          426 a          666 c          641 g          652 t
ORIGIN      
        1 gcctcggact caaagcgccg ctcccaagtg atctggccgg caccattgag gcggaagtaa
       61 aaaccggact ttcggcggag ggcgaatcta tttccatagc gctctatcag atagacagtg
      121 gtaactgcgg cgtagttgtc accagtgtgc cccgcaaagg cggcgaactc gatctcggta
      181 agaatcacga aaacgtgcgc tcaattctcg atatgctgga aaagagctcg atggctctat
      241 agttttttcc ttccctggta tttggcatag tacttagcaa gcttgacttg gtaaactgta
      301 gtctccgttc cttattatat tttgagtata tctagttgct caccgggatt ctttgtctgg
      361 cagtattttt cgttatggcc gtggccatgt acggacgtaa gttgtcagcc cttctggcct
      421 tgccgctcat ggccattgcc atagcactga taggcggcat cgacggcgcc gtcattgtca
      481 aagacgtatt gagtgccggc tcggtcaagt tgcacaatgc ctatactgcc actatgtttg
      541 gtgccatgct ggccgaactg atcaacaagc acggcatggc ccgttcgctg gtgcgttttg
      601 tggctgaatt cgccggtgac agcccttacc tgttgggact gcttttaacg ggcgttaccg
      661 ccctcttgtt ctccactctc ggcggtctgg gtgccgtcat catggttggt actattgttt
      721 taccggtgat gctttccctt ggtataccgg ctctggccgc cgccgggctt ttcttgttcg
      781 gcatctcgct gggtggaatg ttcaacctga ctaactggca gctttacact gatgttctca
      841 aaattgagca ggcaacgatc atttcctttg tggtgccatt ttcgcttatc ctgggcctgg
      901 taattttgct ctttctgctc tttgaactga aagagaaacg caatgccggt tacttcagca
      961 tcggacttct ggtgctgggc gcgctctact atcccttgag ccgtttcaat ccccgagacg
     1021 cttccgcttt tgccgccttt agcgattttt cctacccgtt cgcctttttc tcattgctgg
     1081 ctctttctgc ctatgctctc tttcggcact ttaccaggca ggaaaacgtg cctggattca
     1141 gtctgctcac cccgattgtg ccgcttattt ttgtgcttgt ctttcgctgg gagataattc
     1201 ctgcttttat catgggcatc gcctatggtg cgctcactac ctggaaacgc gactctataa
     1261 atattctcac tcgctccatc atagacggcg ttaccaatgt cataccggcc gtgctcttga
     1321 tgatgggcat cggcatgctg attgccgccg tcatggataa aggagtttcg gcggccatcg
     1381 ctcctctgat caaggcggtt gtgcctggct cacccctcac ttatgttttg acctttacct
     1441 taatggcacc tctcgctcta taccgcggac ccttaagtct ctggggcatg ggcagtggtc
     1501 ttgtcacctt gattatcaac ttgaccacat tgacggccca ggccgtcatg ggcatgctga
     1561 tgtccgttgg tcaagtacag ggtatttgcg atcctaccaa tacccagaac atctggattg
     1621 ctacatacct gggggtcgac acccaggtga tcctgcgtaa aaccctgccc tatgccttgc
     1681 tggcggcctt cctcggtctc agcctggctg tcctgaaggg gtatgtgcag tgagtaacac
     1741 ctatcgaatc ggcattgatg tgggcggcac tttcactcat gccgtggcta tcgacggact
     1801 cacttccaat ctggtcggga aggcgaaagt gcctaccagc cattcggcac cggaaggggt
     1861 ggcgcggggt atcgtcgagt cattgcactt gcttctggcc gatacgggca tcgcccctga
     1921 taccgtctcc tttatcgccc acagtaccac ccaggctacc aatgcgctcc tggagggcga
     1981 cgtggtcaag gtcggtgtgc ttggtatgtc ctcgccggcc cttgctcccc tggcctccgc
     2041 cgccaccagg cttggacgca tacccctggc cgggcacggc gccaagggcg tttatatgga
     2101 gaccgcccat cgctttctgg ctgtggaatc cagagcctcc ggtacggcga ccggcgagct
     2161 tgatgcttcc caactggaac aagtagagaa actgctcctg gagttgaagg ccgagggttg
     2221 cggggccatt gccgtttccg atgcttacag cgtcgatgac ggtaagcggg aggaagcggt
     2281 gctggccgtt tgtgggcgtc ttggtttgct tgccacctcc ggttccgagg tgagcaggct
     2341 ctatggtttg aaggtgcgta cccgcacggc ggccatcaat gccgg
//
LOCUS       DAOQNZ010000592         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26822_length_2385_cov_3.538627, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000592 DAOQNZ010000000
VERSION     DAOQNZ010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26822_length_2385_cov_3.538627"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKW73_10925"
     CDS             complement(<1..475)
                     /locus_tag="PKW73_10925"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNA73845.1"
                     /translation="MNYPIWVVPYLGGPWVIGIISIVHIFLSHFAVGGGAFLALAEQI
                     AYKRKNENLYNYLKGHSRFFLIITTVSGAVTGVGIWFSISLVSPDGTASLIQSFTLAW
                     ALEYLFFVAELATAFVYYYTWDRVSKETHLLLARLYMVFSIMTLVIINGIITFMLT"
     gene            complement(472..1557)
                     /locus_tag="PKW73_10930"
     CDS             complement(472..1557)
                     /locus_tag="PKW73_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNA73846.1"
                     /translation="MVGDTRRGNKLLGFLEDRFALSTLLHLAEKKKVPIHRHSVWYYM
                     GGIAAIYLAVQVVTGILLMVYYVPEIQSAHASILKINSQIDFGWFIRSLHSWGANLMI
                     AVVVLHLFSTYFMKAYRSPREFTWLSGLLLLVLCMAFGFTGYLLPWDDVSFFATKIGL
                     DVAAKTPVLGGIIADLLRGGEAISQATISRFFVIHVAVLPICLLGLLGGHLLFVQMHG
                     MSEPDAFKNLPSAKKTYEKFFPNFFFKDILVWILALNVLFVLVMLCPWGIGPEADPFG
                     AAPIGIKPEWYFLSMFQFLKLVPPMIGPIEGELFGILLMGIAALAFAVLPLLDQGKSN
                     SMSKLATAYGVIIAIAAIALSVWGALS"
     gene            complement(1591..2148)
                     /locus_tag="PKW73_10935"
     CDS             complement(1591..2148)
                     /locus_tag="PKW73_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNA73847.1"
                     /translation="MEDNSATKIEEEDKSSENPAAGDSHAETDSHWDLDILGEGQVGR
                     GTMMRTAMGGIALLWGGMTLYPIYSYLKPKASDKDEQTKVTSLEVCKVDELPKGSGRN
                     FRFGSTPALVIHTPDGEIHAFKAICTHLGCTVQYRNDKDAIWCACHGGQYDSHTGKNV
                     AGPPPKPLAPLKAEVVNGKIVVSQA"
     gene            complement(2175..>2385)
                     /locus_tag="PKW73_10940"
     CDS             complement(2175..>2385)
                     /locus_tag="PKW73_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73848.1"
                     /translation="IFNRHQLRVWLLTHVQPSTVPVATQWDLLITFVVSAVALIVYLV
                     WLSKLCVKAFAAPVQAPDGERLAAQ"
BASE COUNT          668 a          617 c          613 g          487 t
ORIGIN      
        1 gtgtaagcat gaaggtgata atgccgttga tgatgaccag ggtcatgatc gagaagacca
       61 tgtacaaccg ggcaagcaga aggtgtgtct ctttggagac acgatcccag gtgtaatagt
      121 agacaaaggc ggtggccagt tcagcgacaa agaacaagta ttcaagggcc caggccaggg
      181 taaaactctg gatgagagaa gccgtgccgt ccggactgac aagggatatg gagaaccaga
      241 tgcccacccc ggtcacggca ccggatacgg tggtaatgat caggaagaaa cgggaatgcc
      301 ccttcaggta gttatagaga ttttcattct tgcgcttgta agcaatttgc tcagccaggg
      361 caagaaaggc tccgccaccc acggcaaagt gggacaagaa aatgtgaaca atagatatta
      421 tgccgataac ccagggtcca ccgagataag gcacaaccca gattggataa ttcatgacag
      481 agctccccaa acactaagtg caatagcggc tattgcaatg atgacaccat aagcagttgc
      541 aagtttagac attgaattcg acttaccctg gtctagcaaa ggcaagacgg caaaggcaag
      601 agcggcaatg cccataagta aaatcccgaa gagttcgccc tcgattggac caatcatagg
      661 cggtaccagt ttcaagaact ggaacatgga caggaagtac cactcgggct taataccgat
      721 tggcgccgca ccaaagggat cagcctcggg accgatgccc cagggacaga gcatgaccag
      781 tacgaaaagc acgttcaaag caagaatcca gaccaggata tctttgaaga agaagttcgg
      841 gaagaacttt tcataagtct tcttcgctga cggcagattc ttgaaagcat ccggttcaga
      901 catgccgtgc atctgcacaa agagcaggtg cccacccagc aagcccaaca agcagatagg
      961 caacacagcc acgtgaatga caaagaaacg ggaaatggta gcctgtgaaa ttgcctcgcc
     1021 gccccgcaag agatcagcga taatgccgcc cagaaccggt gtcttagccg ctacatcaag
     1081 accaatcttg gtagcaaaaa agctgacatc gtcccagggc agaaggtaac cggtgaaacc
     1141 aaaagccata cagagcacta acaagagaag accggagagc caggtgaatt cacggggact
     1201 gcggtaggct ttcataaaat aggtggagaa caaatgcagc accaccacgg caatcatcaa
     1261 attggcgccc cagctatgca gggagcggat gaaccaacca aagtcaatct gagaattgat
     1321 cttgagaatg gaagcatggg ctgactggat ctccggcacg tagtagacca taagcaaaat
     1381 accggtcacc acctgcaccg ccagatagat agcggcaatc ccacccatgt agtaccagac
     1441 cgaatgccgg tggattggta ctttcttctt ctcggccaag tgcaagagcg tgctgagagc
     1501 gaatctgtct tccagaaagc cgagcagttt attacctctg cgtgtgtccc ctaccatgat
     1561 gattccttgt cctcttcaat atcagttagt tcaagcctgc gacaccacga tcttgccgtt
     1621 cacgacctcg gctttcaagg gcgccagcgg cttaggcggc ggtccggcaa cgttcttacc
     1681 ggtatgcgaa tcgtactgcc cgccatggca ggcacaccaa atagcgtctt tatcgttgcg
     1741 atactgcact gtgcaaccaa gatgggtgca aatggccttg aaggcatgaa tttcgccgtc
     1801 aggcgtatgt attacaagag cgggagtaga accgaaacgg aagttacgcc ccgagccctt
     1861 gggtaattca tcgactttgc aaacttccag gctggtgacc ttggtctgct catccttatc
     1921 ggaggctttg ggttttagat aactatatat aggatagagg gtcatgccgc cccagagcaa
     1981 agcaattcct cccatggcgg tgcgcatcat tgtgccccga ccaacctgtc cttcccccag
     2041 aatgtccagg tcccagtggc tatctgtctc tgcatgacta tcaccagccg cagggttctc
     2101 tgaggactta tcttcctctt caattttggt ggctgaattg tcttccatct tccggtttcc
     2161 tctttccaat ttttctactg ggcggctaaa cgctcaccgt ccggtgcctg aaccggcgcc
     2221 gcaaaggctt tcacacaaag cttactgagc cagacaagat aaacgatcag ggccactgct
     2281 gaaaccacaa aggtaatgag caagtcccac tgcgtggcca ccggcacggt gctgggctgt
     2341 acatgagtga ggagccacac tctcagctga tggcgattga agatc
//
LOCUS       DAOQNZ010000593         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26849_length_2384_cov_3.117218, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000593 DAOQNZ010000000
VERSION     DAOQNZ010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26849_length_2384_cov_3.117218"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2384
                     /locus_tag="PKW73_10945"
     CDS             <1..>2384
                     /locus_tag="PKW73_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73849.1"
                     /translation="SRAASLILAAHYDLSSGSEKEKLAAALKESATTPAMDLHLIPGQ
                     TATRFDGKAAAMEALAGNQALREEIEALEQPQPQAQKEQDQKLNTNTIEQLVKEQGRE
                     VESASKKERGERPEHIQRVESSEGADYALRVVNANGEISEYHFNRADKGSKQESEKDT
                     LVAVKSGGTMTSSLMPVIDFKTHLKKPPHATDAWMVIQAEGHKSGEMLYEKHSLDRNT
                     GTETVEGKDGGKLVKRPDGVTVVTRDGITSTTLKLESGVTIEGDQGGAFRAMQFQDGS
                     RIESLPGGRFRQLRPDSEGKLKEESIFSGHLQLTTPGRQLQIVEERQDGARLFRQDGA
                     VMTFSKNGSELEIQRGGVQRQGLKIGAFDEQGKPTKIYSEKGDGSYSSTEDGITWKGY
                     DANGKLIPGSEKKEVVVLDLAAGVIGHLSEDRSKVVEERFDGSLKEYEAVGKEGLVVT
                     REVSAGGTTVQHSYKDGRLSETKESTGSGAMRTWQNLSLKGQDGQRLDYPVLTARRSA
                     AEHGGKEMTFTYETDTKPGAIPKITSYTDEQGKWSAKNGAPETGIYINERTGDHRVES
                     LAVTMDGRLKRVSQVQNDVIGLGNDLETSREITTMDGTVIAFPASGGLLLRDKDGNAA
                     RTISDTGAAKDYSYNEAGLLNKVVETDATGKTNTWEASIGHMLSTWRDSTGKVEHGDW
                     RISKTTGQEFFIFEKGASYSRDAGQDKYKQVSDGKEAREKVDALMTAYEDWYLTSGRR
                     FEALRSNLENMTPDQIMMFRRAFKEKHSDDYATFGHYIGEKFGQSARGEILTDM"
BASE COUNT          716 a          618 c          674 g          376 t
ORIGIN      
        1 agagccgagc ggcaagcctg atcttagcgg ctcactatga cctttcctca ggtagcgaaa
       61 aggagaagct ggctgccgct ctcaaagaga gcgccaccac cccggccatg gatttgcatc
      121 tcatccccgg ccagaccgcc actcgttttg atgggaaagc cgctgccatg gaggccctgg
      181 ccggaaatca ggctctcaga gaggaaatag aagctctgga gcagccgcag cctcaagcgc
      241 aaaaggagca agaccaaaaa ctcaatacca acaccatcga acaacttgtg aaagaacagg
      301 gtcgcgaggt agaaagcgcc tcaaagaaag agcgcggcga gagaccggaa catattcaaa
      361 gagtggaaag ttcggaagga gctgactacg ccttgcgcgt cgtcaacgct aacggtgaga
      421 taagcgaata ccacttcaac cgcgccgaca aaggaagcaa gcaagaatct gagaaagaca
      481 ctcttgttgc agtgaagagc ggcggcacca tgacctcctc tctcatgccg gtaattgatt
      541 tcaagaccca tctcaagaaa ccgccccatg ccactgacgc ctggatggtc atacaggcag
      601 aggggcacaa gagcggcgaa atgctctatg aaaaacacag cctggacaga aacacaggca
      661 ccgaaaccgt tgaaggaaaa gacggaggca aactagtcaa aagaccagac ggcgtaactg
      721 tggtaaccag agacggtatc acttccacta ccctcaaact ggaaagcggc gtcaccatag
      781 aaggtgacca gggcggcgcc tttagagcca tgcaatttca agacgggagt cgaatagaga
      841 gtcttcccgg tgggcgcttc cggcagctaa gaccagactc cgagggcaag ctgaaggaag
      901 agagtatctt ctccggtcac ttgcaactaa ccacaccggg ccgacagctg cagatagtgg
      961 aagagcgtca ggacggggct cgtcttttca gacaagacgg agccgtcatg actttcagca
     1021 agaacggctc ggagttggaa atacagcgcg gcggcgtcca gaggcaaggg ctcaagatag
     1081 gagcctttga cgaacaaggc aaacccacaa aaatctattc ggagaaaggc gacggctcct
     1141 acagcagcac cgaagacggt attacatgga aaggttatga cgcaaacggc aagctcatac
     1201 caggcagtga gaagaaagag gtagtagtac tggatctggc cgccggcgta atcggacacc
     1261 tttcagagga ccgcagcaag gtcgtggaag aacgcttcga cggctccctc aaagaatacg
     1321 aagcagtagg gaaagaggga ctggtggtaa cccgagaagt gagcgcgggc ggcaccacgg
     1381 tgcaacactc ttataaagac ggcagactat cggaaacaaa ggaaagcacc ggcagtggtg
     1441 ccatgcgcac ctggcaaaat ctcagcctga aaggtcagga cggacagagg ctggactatc
     1501 cggtacttac agccaggcgc agtgccgcag agcatggcgg caaggaaatg acctttactt
     1561 atgagactga caccaaacca ggtgccatac ccaaaattac aagttacaca gatgagcagg
     1621 ggaaatggtc ggcaaaaaat ggagcaccgg aaacgggtat ttacataaac gaaaggaccg
     1681 gcgatcaccg ggtcgagagc ctggccgtga ccatggatgg acggctgaag cgggtcagtc
     1741 aagtacaaaa tgacgtgatt ggccttggca acgacctgga gaccagtaga gaaatcacca
     1801 ccatggacgg cacggtcatt gcctttcccg ccagcggagg cttgctgctt cgggataaag
     1861 atggaaatgc cgcccgcacc atcagcgata ccggcgccgc caaagactat tcctacaatg
     1921 aagccgggct tttaaacaaa gtagtggaaa ccgatgccac aggcaagacc aacacctggg
     1981 aagccagtat cggtcatatg ctctccactt ggagagactc caccggcaaa gtagaacatg
     2041 gggattggcg aattagcaaa accaccggtc aggaattctt catcttcgag aaaggcgcaa
     2101 gttacagccg ggacgccgga caggacaagt acaaacaggt cagcgacggc aaggaagcaa
     2161 gagaaaaggt cgatgctctc atgacggcct atgaagactg gtacttgacc agtggtcgcc
     2221 gcttcgaggc cctgcgcagc aacctggaaa acatgacccc cgatcaaatc atgatgttca
     2281 ggcgcgcctt caaagagaaa cattctgatg actatgccac cttcggtcac tacatcggcg
     2341 aaaaattcgg gcagtcggcc cggggggaaa ttctcactga catg
//
LOCUS       DAOQNZ010000594         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_26892_length_2382_cov_3.776966, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000594 DAOQNZ010000000
VERSION     DAOQNZ010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26892_length_2382_cov_3.776966"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..432
                     /locus_tag="PKW73_10950"
     CDS             190..432
                     /locus_tag="PKW73_10950"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNA73850.1"
                     /translation="MFIPVIGLLVVGLIAIYIHKSGVNRYENGSEMLGGVCQGLAYRT
                     GLPVALVRILTVLFALGTAGEVVLLYVLLWATLPRG"
     gene            523..792
                     /locus_tag="PKW73_10955"
     CDS             523..792
                     /locus_tag="PKW73_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73851.1"
                     /translation="MDSILHYTDPTLTLVATFSFVVCFLLGVWSGARQGGRVILPSLI
                     LIAISVYIRSQFPPLSMAAITAVSLMHVGAGALTALLVGRSKSKP"
     gene            912..1397
                     /locus_tag="PKW73_10960"
     CDS             912..1397
                     /locus_tag="PKW73_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73852.1"
                     /translation="MEKSHIRKAFDATFEKPALPVLGLLFAALVLFFFRLHVLALIFV
                     LAAMPFYVMMFRQDAFRRRSIATETSKTDEQLHLELLELQKLAIAKLGNADLQLQVRV
                     ADLLIDAAKHLGYPYLGGRRVYAEDATLVWNKEGLALAAQARALIEEYLQNQSESGSA
                     A"
     gene            2145..>2382
                     /locus_tag="PKW73_10965"
     CDS             2145..>2382
                     /locus_tag="PKW73_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73853.1"
                     /translation="MREHQISINLKAQQFELLQRLAREKGYKSVSAYVREKILELALS
                     MEPPSRSAGASANPIEPRVYKELARIHGDLKLLIE"
BASE COUNT          523 a          581 c          614 g          664 t
ORIGIN      
        1 acagagccgt tgcgagttgt ctgcttcgga ccgaacagtg cagatggggc gcctcattac
       61 taatggactg cgttcgtctc tattggagtc ggcgaaaaat ggcaaaggga cttcaaaaac
      121 aggaacgctg cttgcctgaa gcggcgctct tcaaccaatt caatcaatta ttgcaacagg
      181 agacatctta tgtttatccc agtcattggt cttttagtcg tgggcttaat cgccatttac
      241 attcacaagt ctggtgtcaa tcgctacgaa aatggctcgg aaatgttggg cggagtatgt
      301 cagggtctgg cttacagaac cggtttgccg gtagcactgg tgcgtatcct gacggtactt
      361 tttgcccttg gcaccgccgg tgaggtcgta cttctgtatg tgcttctctg ggcaactctg
      421 ccgcgtggct agtagaacga aaaaaattac tccaaatagt gcttcctcgc ccgggggagc
      481 accaaaatca aagacaaata aatgctgagg caaattccaa atatggattc catccttcac
      541 tacaccgatc ctaccttaac cctcgttgcc accttctcct tcgtcgtttg cttccttctt
      601 ggcgtatgga gcggcgctcg tcagggtggg cgggtcattc ttccctccct gatcttgatc
      661 gcaatttcag tctacattcg ctctcaattt cctcccctca gtatggccgc cattaccgct
      721 gtcagcctga tgcatgtggg cgccggggcc ttgactgctc tactggtggg gcgttccaag
      781 tcaaagccat agtaaatgca gtcgctcccc tgattgtttg ggggagtttc tgatctcttc
      841 ttgttctcat caagccttag ctgcttgagt caccttgaac aattccttca acatcatctg
      901 gagattccat aatggaaaaa tctcacattc gcaaggcctt tgatgccacc ttcgaaaaac
      961 ctgccttacc tgtcttgggc ttattgtttg ctgcccttgt gttgttcttt ttccgactgc
     1021 acgtactggc cttgatcttt gttctggcgg ccatgccttt ctatgtcatg atgtttcgcc
     1081 aggatgcttt caggcgtcgg tccatcgcta cagagaccag caaaactgat gagcagttgc
     1141 atctggagct tttggaattg cagaagcttg ccattgccaa actcggtaat gccgatcttc
     1201 aattgcaggt ccgcgtcgcc gatttgctga ttgatgccgc taaacatctg ggctatccat
     1261 atcttggcgg caggagagtg tatgcggaag atgctacttt ggtttggaac aaagaagggc
     1321 tggcactggc cgcacaagct agagcgctga ttgaagagta tctgcagaac cagagtgagt
     1381 ccggaagtgc ggcttgaatt tgagtagttc cttctaaaat gattgaaatc tgcatcaggt
     1441 gcagatttcg catgagcttt tccgggctga ggcgtgtaat tgcttctgcc cggatcagcc
     1501 gtgctcctgt ttgctagcgc ctgaacttgc tccttttatt gctgcttttg gctttcaatc
     1561 gggtctgtgt gggtaattac cgtttttttg tttttttgcg ccatgatatt ataaaatgta
     1621 gtatgatgcg caaaaagaac cagtggtggg ctctccggtc gtcctggggg cgggggtgga
     1681 ttcgtagacc ggtcgtctgg atttctccca aaaagagaag ggttggagtg gaaaagaaag
     1741 gcttccctgc ctattcttca ggactaaaat tagtgcctca ctatcttcaa gcttgctctc
     1801 gtcaagcttg cgaagactcc agatggggcg tggcgcatga ccggtgccgg cgaaagtgtg
     1861 tgccgtagcg caagcgcttg ctccatctgc ctctccagaa ttctcgaccg cgacctactt
     1921 tcagcaaata acgtagctgt gattttcttt cagttgtttg gtgcaaaaga tcgctcgagt
     1981 gctgaatatt cgtaatcccc aggaaaacta gagccaattc cagtcccgag gtaagaatgt
     2041 tgccaagact ttcgcagagc tgaacaataa gccttcggct gggggcattt attcgggcct
     2101 tcgctgttgc gctagaatta gactcagagg tcgggagtga cgttttgcgc gagcatcaga
     2161 ttagtatcaa tttgaaagct caacagtttg agctgttgca gagactggcc agggagaaag
     2221 gctacaagtc ggtaagcgcc tatgtgcgag agaaaatttt ggagctggcc ctttccatgg
     2281 agccgccctc gcgaagcgcg ggagcgtccg ccaatcccat tgaacccagg gtctataagg
     2341 aactggctcg tattcacggc gatctcaaac tgctcattga ag
//
LOCUS       DAOQNZ010000595         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27026_length_2376_cov_3.838001, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000595 DAOQNZ010000000
VERSION     DAOQNZ010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27026_length_2376_cov_3.838001"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PKW73_10970"
     CDS             <1..483
                     /locus_tag="PKW73_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73854.1"
                     /translation="NFDEVASSFVEEALAMPNRQINLLAHRPGGTHYETKELETRRIH
                     KLTDEEASFIFLEVSLSDPSEFFDNCLRVEGHLINGHKVLRCHSAAIPNAIAAILLHL
                     RDETKTIPHAYFGWTEGNPLSYVFRYIFLGEGETAPVTREILREVVKEPHLRPVVIVG
                     "
     gene            588..1325
                     /locus_tag="PKW73_10975"
     CDS             588..1325
                     /locus_tag="PKW73_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HNA73855.1"
                     /translation="MVKSKSRGFVPGSRYLLALLYLIGGLACLFIVSKAAYHPAVCLL
                     LYGGAALSLSLFSGAVINIMKGDQLVETSPESLSLTTRKRLRRLALCLPLPIIGYLAG
                     SLALSVYARQAEDLSPLLQVKRQEEALLGTSFTWQLLPQNIKERAEAQVQADSGYFMQ
                     SLQQSIKEHWQPQSNGTTYRAAVAFQVNSQGRIVNDRIMEVFGDGVDVDAIKAALEGA
                     SGTTPLPPSLEAPVDVDFTFEYNYMTR"
     gene            complement(1337..1678)
                     /locus_tag="PKW73_10980"
     CDS             complement(1337..1678)
                     /locus_tag="PKW73_10980"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNA73856.1"
                     /translation="MDFFHFCFYLIVSAVCAGLAARVLPGSKVKDRAKEVFSPAGFLS
                     KAVVGIIGAWLGEMVLGPIGPSLEGVRLVPAVVGSVFFVLALSLVSLLVSKIVAVCNL
                     PSLIKGRQTSR"
     gene            1816..2262
                     /locus_tag="PKW73_10985"
     CDS             1816..2262
                     /locus_tag="PKW73_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73857.1"
                     /translation="MTTRPLPGTFSLLLCISVLSTAMTLSGCGNSDNSSPSVTKQEET
                     SVTVGADGKTTVVEKESEKKADDDDSSNQATESKDNSVKVEAGGSDGDTRVKVNLPGI
                     KVDIDESKGSVNVSTPYADIKKDPGKSKATVEINLDSSDAKGDSEK"
BASE COUNT          621 a          692 c          613 g          450 t
ORIGIN      
        1 aacttcgacg aggtggcctc cagcttcgtt gaagaagcac tggccatgcc caaccggcag
       61 atcaatctac tggcccacag accgggcggc acccactatg agacgaaaga actggagacc
      121 cgccgcattc acaaattgac cgacgaagaa gccagcttta tcttccttga ggtgtccctc
      181 tccgatccct cggagttttt cgacaattgc ttgagagtgg aaggacatct catcaacggg
      241 cataaagttc tgcgctgcca tagcgcggcc attcccaacg ccatcgccgc catcttgttg
      301 cacttgcggg atgagaccaa gacaattccc cacgcatact tcggctggac cgagggtaac
      361 cccctgagct atgtctttcg ctacatcttc ctgggcgaag gcgaaaccgc accggtgacc
      421 agggaaattc tcagagaagt ggtaaaagag ccccacttgc gaccggtggt catcgtaggg
      481 tgaaggctca gggtttttcc cagtgcttcc ggcgggttgc caagatattc tgctaacaag
      541 ctttattgct gcgggaattt tcttaagtgc tttgccggga gacgcccatg gtcaaaagta
      601 aaagccgcgg cttcgtgccg ggcagtcgct acctgcttgc cctgctttat ctaattggcg
      661 ggctggcctg cctcttcatc gtttccaaag ccgcctatca tccggcagtt tgcctgcttc
      721 tgtacggcgg cgccgccctt tcgctatcgc tcttttcagg ggcggttata aacataatga
      781 agggagatca actggttgaa accagcccag agagcctcag cctcactact cgcaaacgac
      841 tgaggcgcct ggccctctgc ctgccgctac ccataatcgg ctacctggca ggcagcctgg
      901 ccctttccgt ctatgccaga caagccgaag atctctcacc gcttttgcag gttaagaggc
      961 aggaagaagc cctgcttggc acctccttca cctggcagct tctgccccag aacatcaagg
     1021 agagggcaga ggcacaggtg caagcggaca gcggttactt catgcaaagc ctgcaacagt
     1081 caatcaagga acactggcag ccgcaaagca atggcaccac ctacagggca gccgtggcct
     1141 tccaggtgaa ttcgcagggg cgaatagtca acgatagaat tatggaagtc ttcggagacg
     1201 gcgttgacgt ggatgcaatc aaagcagccc tggaaggggc ctccggcacg acaccgctgc
     1261 cgccctccct ggaggctccc gtggacgtgg atttcacctt cgagtacaac tacatgacca
     1321 ggtaagagcg gccgcctcag cgactggttt gccgcccctt aataagcgaa ggtaaattgc
     1381 agaccgccac tatcttcgat accagaagcg acaccagcga cagggccagc acaaagaaaa
     1441 ccgaaccaac aacggccggt acaagacgca cgccttccaa actggggcca atcggaccaa
     1501 gcaccatctc gcccaaccag gcgccgataa taccgaccac cgccttggag agaaagcccg
     1561 ccggcgaaaa aacttcctta gcccggtctt tcactttact accgggcaga acccgcgccg
     1621 ccagcccggc acagactgcc gagacaatca aatagaagca gaagtggaaa aagtccatga
     1681 aactccagat tcggttttaa cagagagcta tgatagcctt ttagctaaaa tcttgtgaca
     1741 ctaacatatt aattcggcca ccgctatcgg ccaagaaaag cgccagaacg ccctaccgcc
     1801 ttaaaggaaa cgacaatgac cacgagacct ctgcccggaa ctttcagcct tctcctctgc
     1861 ataagcgttc tgagtaccgc catgaccctg agcggctgcg gaaacagtga taactccagc
     1921 ccgtccgtca ccaagcaaga ggaaacttca gtgacggtgg gagctgacgg caaaaccacg
     1981 gtggtggaaa aggaatcgga aaagaaagcc gacgatgatg acagtagcaa tcaagccacc
     2041 gaaagcaaag acaattcggt gaaagtggaa gccggcggtt cagacggcga caccagagta
     2101 aaggtcaatc taccgggcat aaaggtagat atcgacgaat ctaaaggttc ggtcaacgta
     2161 tccacgccct acgccgacat caaaaaagat cccggcaaat caaaggctac agtggaaatc
     2221 aatctggata gttccgatgc aaaaggggat tctgaaaaat gacggtcaaa ttcttcctgc
     2281 cgtattctct gactctgctc tgtgcaatgc tttgcgccgc agacagccgg gccctggcaa
     2341 gcgaaccggg ccagggtcag ctcctggcag gaaaga
//
LOCUS       DAOQNZ010000596         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27133_length_2370_cov_3.846652, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000596 DAOQNZ010000000
VERSION     DAOQNZ010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27133_length_2370_cov_3.846652"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKW73_10990"
     CDS             complement(<1..247)
                     /locus_tag="PKW73_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73858.1"
                     /translation="MNSEASEEPAKPSEAGADSNASPNPPYKNVKDIIKGFRKDVYHA
                     ISDQPLSNKQVLEFENRVLFNPDDSEAREHLAYHYRKQ"
     gene            complement(439..1200)
                     /locus_tag="PKW73_10995"
     CDS             complement(439..1200)
                     /locus_tag="PKW73_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73859.1"
                     /translation="MSIDGYVYVPEESKEAMTQASQGARVSLSDGKPPFIVVDHYLES
                     IVVAQWPGKLWHVRILEPLTKAAEQAAGVAPLIAGATYTRALSVEVLDEIPIARLFGN
                     CGEAVCAIISQAGQMQIEQVHSLAAARHPAAGATYARLWKNWLASISHVTSTEPPDDV
                     ATVSVFVGQGRPKSPVNEGFSVLYQEMINRAESVSGAAAFIEEEEDRYLEPNWSAATK
                     TLLDATMAYGAPQLCTQEDCTILTQAWCSVIGPLS"
     gene            complement(1221..1715)
                     /locus_tag="PKW73_11000"
     CDS             complement(1221..1715)
                     /locus_tag="PKW73_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNA73860.1"
                     /translation="MQQETFTQASLKELTEYVVARHHAFLRRQLPAISSLFQSAQDNS
                     STAPATVADAGNIFTTVRTKIETHLRDEEQFLFPNGVSLAEGGVAEVCELDLKGRLEE
                     MEREHDNAGLALENCRRLLINEASTDSKLKELVIALTSLIDDLSEHVRIENTYIHPIL
                     KKYL"
     gene            complement(1805..>2370)
                     /locus_tag="PKW73_11005"
     CDS             complement(1805..>2370)
                     /locus_tag="PKW73_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73861.1"
                     /translation="SSAANQRSFESKRCTNGVFTRHLISALQKQGQNTTITEAFNYLK
                     EQVENEVRFDRQLTQTPVMRSKWSGGELILVAVPTKPRQIEPATSAATDADSEVKPDR
                     SAIPNLAGDWLASYGAHYQIWQNGREYGWKFPHEEGRGVIADDGKTARCRWWGKNNGT
                     DTATLEYDSSGMVVRIVGSNGVTLTRQ"
BASE COUNT          521 a          612 c          590 g          647 t
ORIGIN      
        1 cctgcttcct gtaatggtaa gccaggtgtt ctcttgcttc ggaatcatct ggattgaaga
       61 gtactctatt ttcaaattcc agcacttgct tattgctgag cggctgatcc gaaattgcgt
      121 ggtacacatc tttgcgaaag cccttgataa tgtctttgac attcttgtag ggcggattcg
      181 gcgaagcatt ggagtccgct cccgcctcgg aaggttttgc cggttcctcg cttgcttcag
      241 aattcataaa cccgttcctg ccagcattac cagctgaggt tcagattcca gttgaaatga
      301 catgcactct caacctgccc cgtttctgcc ttataccatg catgcaacta acttctttcc
      361 acaatgagag cggtttgaaa ctaaatggta tgaccgacag agccgaacgc tcccggctaa
      421 aattcatttg gcagtcggct agctcaaagg accaataact gaacaccagg cttgcgtaag
      481 aatagtgcag tcctcttgcg tgcagagttg cggcgctccg tatgccatgg ttgcatccaa
      541 taatgtttta gtggcggcag accagtttgg ctccagatat ctatcttcct cttcctctat
      601 gaaggcagcc gctccggaaa ctgattcggc gcggttgatc atttcttgat agagcacgga
      661 gaagccctcg ttaaccggtg acttgggacg cccctgacca acaaagacgc tcactgtcgc
      721 tacgtcgtcc ggtggctctg tgctggtgac atgactaatg gatgctaacc aatttttcca
      781 gagccgtgca taggttgcac cggcagcggg gtggcgagcc gctgccaggg aatggacttg
      841 ctctatttgc atctgtccgg cttgtgaaat gattgcacag acagcctctc cgcagttacc
      901 gaatagcctt gcaattggga tctcatctaa gacttcaacc gaaagggccc gggtgtaagt
      961 tgcaccggcg attagtggag ctacacctgc ggcttgctct gccgctttcg taagcggttc
     1021 cagaatgcgg acatgccaga gtttgcccgg ccactgtgcc accactatcg attccaggta
     1081 gtgatccaca acgataaaag ggggcttgcc gtcagaaagg gaaaccctgg caccctggct
     1141 tgcttgtgtc atagcttctt tgctttcttc aggaacgtaa acgtaaccat ctatcgacat
     1201 tcgcttgcca ccgccctgga tcaaaggtac ttcttaagga tgggatgaat gtaagtgttt
     1261 tcgatacgca cgtgctcact gagatcatcg atcaaacttg taagagctat gacgagttcc
     1321 tttagtttgg agtctgtgct tgcttcattg ataagcaggc ggcggcaatt ttccagggcc
     1381 agtccggcat tgtcatgttc ccgttccatc tcttccaatc tgcctttaag atcaagttca
     1441 caaacttccg ccacgccgcc ctcagcgaga gaaactccgt taggaaacaa gaactgttct
     1501 tcatctcgaa gatgcgtttc aattttggtg cgcactgttg taaagatatt gcctgcatca
     1561 gccaccgttg ccggtgctgt ggacgaatta tcttgggctg actgaaacag cgaggatata
     1621 gccggtaact ggcgtctgag aaaggcgtgg tgcctggcca cgacgtattc agtaagctct
     1681 ttcaaggaag cctgggtgaa tgtttcttgt tgcatcaatt attgccgcca gtgctgatga
     1741 ccacgaaaag catagcaggt gatctggctc ccgaactgaa ttcagtttgg cttgccggca
     1801 atattcattg acgagtcagg gtcacaccat tactgcccac tattcgcaca accatgccgc
     1861 ttgaatcgta ctctagtgtg gctgtatcgg taccattgtt ctttccccac catcggcatc
     1921 tggcagtttt tccgtcgtcg gcgatgacgc cgcgcccttc ctcatgcgga aacttccacc
     1981 catactctct gccattttgc cagatttgat agtgtgctcc atagctggcg agccaatcac
     2041 ctgcaaggtt tggaatagca gaccggtcgg gtttgacttc cgagtcggca tctgtcgccg
     2101 cagaggtagc aggctcaatt tgccggggct ttgtgggaac ggcgactaat attagttcac
     2161 ctcccgacca cttcgagcgc attaccgggg tctgagtcaa ttgtcgatcg aatctgactt
     2221 cgttttccac ttgttctttc aagtaattga aagcttcggt aatagtagtg ttttgtccct
     2281 gcttttgcag agcagagatt agatgcctgg tgaatacgcc attggtgcat cgtttggact
     2341 cgaatgatct ctggttggcc gcacttgaac
//
LOCUS       DAOQNZ010000597         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27161_length_2369_cov_3.803371, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000597 DAOQNZ010000000
VERSION     DAOQNZ010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27161_length_2369_cov_3.803371"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKW73_11010"
     CDS             <1..199
                     /locus_tag="PKW73_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNA73862.1"
                     /translation="SQLTPRERDIMHLRYGLEDGRQRTLEEVGRLFNITRERVRQIEH
                     KAFRKLRQPNWSSKLAGYLED"
     gene            362..1747
                     /gene="mgtE"
                     /locus_tag="PKW73_11015"
     CDS             362..1747
                     /gene="mgtE"
                     /locus_tag="PKW73_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNA73863.1"
                     /translation="MTEKQLLAPEMLFDLVDTNQRQKLRLILNDQHSADLAECFEELP
                     PSYRISCFRVLDLDNASALLAELEPDKQSELLRDLGNNSIAALVVNMSPDDAVDLLSE
                     LPETKAREIIDQVLDAEVKEDLTQLMTFKEDTAGGIMSTDYLALKSGMTVAEALVYLR
                     DQYEDLEEEIYDVYVVGSNEELVGVVTLKELLTASPEVLVHSIMDEDLVYVEADVDQE
                     HAAEKLSRYDLLTLPVVNQHKQLLGIITADDVIDVLKEEATEDIYQSSGISAGSETQE
                     QLGYNVRRAFSARLPWLLVTLMIETGSASVITHFDSVIQQTVQAAAFMPLLSGVTGSV
                     ATQSTCIVIRGTGRTAGFGLKAALRNIWHEVKVGVLLGLFCGLSCYLVSTFMGSSSQA
                     LGIVVATSLFITMTVGVLLGTLMPMLFQKLGVEPAHASGPFITSILDVCTMSIYLTIV
                     HLFLSHFHSGF"
     gene            1842..>2369
                     /locus_tag="PKW73_11020"
     CDS             1842..>2369
                     /locus_tag="PKW73_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNA73864.1"
                     /translation="MEKTMEKISRDPLADMLKRREEAAAVNQAQVDSRHQKGILTARE
                     RIEKLVDPQSFMEIDRYALHQCREFGMDEKRNLGDGVITGQATIDGRPVYLFAHDATF
                     VGGALGEVFARKVCKVMDLADQAGCPVIGLNESGGARIQEGVRSLAGYADIFYRNVKS
                     SGVIPQISVIYGACAG"
BASE COUNT          579 a          641 c          622 g          527 t
ORIGIN      
        1 cagccagctt acgccccgag aaagagacat aatgcatctg cgttacgggc tggaagacgg
       61 acggcagcgc actctggaag aagttggacg gctcttcaac attacccggg aacgtgttcg
      121 tcaaatagag cacaaggcgt ttcgtaaatt gagacagcca aactggtcat ccaagctggc
      181 cggttacctg gaagattaag ttgcacctgt aaatatttca ccgtctgagc gccgctccat
      241 cacaattttc ttaaagcatt agacgaaggc tcttcttgac tggcttaatg cttgctaggg
      301 ttatgctctg accattcatt cagatgaatg gcctccgcgt gagaaacaga agttggccca
      361 ggtgacggaa aagcagctac ttgctcccga aatgctcttt gatctggttg atacaaacca
      421 gaggcaaaag ctcaggctca tattgaatga tcagcactcc gccgacctgg ccgagtgctt
      481 cgaagaactg cctcccagtt atcgcatctc ctgctttcgg gtattggacc tggacaatgc
      541 atcggctctt ctggccgaac tggaaccgga caagcaatcc gaacttttga gagacctggg
      601 aaataattcc attgcggcgc tggtggtcaa catgtccccg gacgatgccg tcgacctgct
      661 ttccgagttg ccggaaacaa aggccagaga aatcatcgac caggttctcg atgcggaagt
      721 aaaagaagac ctcacgcagc tgatgacctt caaggaagac acggccggcg gcattatgtc
      781 cacggactac ctggccttga agagcggcat gaccgtagcc gaagccctgg tctacctgcg
      841 ggaccaatac gaagacctgg aagaagagat ctatgacgtc tatgtagtcg gcagcaatga
      901 agaactggta ggcgtcgtca ccctcaaaga gcttttgacg gcctcgccgg aagtactcgt
      961 gcattcaatc atggacgaag atctagtcta tgtggaagcc gatgtggacc aggagcatgc
     1021 cgcggaaaaa ctcagtcgct atgaccttct gactctgcct gtagtcaatc agcataaaca
     1081 attgcttggt atcatcaccg ccgatgatgt catcgacgtt ttgaaggaag aagccaccga
     1141 agatatctat cagtcatccg gtatttcggc cggctctgaa actcaggagc agttgggcta
     1201 taacgtacgt cgagcctttt ccgccagact cccctggctc ctggtcaccc tcatgatcga
     1261 aaccggatcg gcatcggtta ttacccactt cgattcggtc atccagcaga ccgtgcaagc
     1321 cgcagcattc atgccgctac tctcgggagt gacaggctca gtagccactc agtctacctg
     1381 tatcgtcatt cgcggcacag gtaggaccgc cggcttcgga ctgaaagcag ccctgcgcaa
     1441 tatctggcac gaagttaaag tgggagtctt gctaggccta ttttgcggtc tctcttgtta
     1501 cctggtcagc actttcatgg gcagcagcag ccaggctctt ggaatcgtgg tggccacctc
     1561 tcttttcatc accatgaccg tgggcgtgct tcttggtacc ctcatgccga tgctcttcca
     1621 gaaactggga gtagaaccgg ctcatgccag cgggcccttc attacaagca tcctcgatgt
     1681 ctgtacaatg tcaatttacc ttacaatagt gcatctcttc ctcagccatt tccattccgg
     1741 cttctaggta aacagctacc cggcatgagg gaagagaggc gaatgagaga ttttagcaac
     1801 gtcagcacca gcttaaggac ccgcttaagg aagcaaaagt gatggaaaag accatggaaa
     1861 aaattagccg cgaccccctg gccgacatgc tcaaacgtcg ggaagaagcc gccgccgtta
     1921 accaggccca ggtagatagc cgccatcaaa agggaatcct gacggccagg gagcgcattg
     1981 aaaagctggt agacccacag agtttcatgg aaatcgatcg ctatgcgctg caccagtgcc
     2041 gggaattcgg catggatgaa aagcgtaacc tcggtgacgg tgtaatcacc ggccaggcca
     2101 ccatagacgg cagaccggtc tatctcttcg cccatgacgc cacctttgtg ggcggtgccc
     2161 tcggtgaggt cttcgcccgc aaggtttgca aagtcatgga tctggcagac caggctggtt
     2221 gtccggtgat cgggttaaac gaaagcggtg gtgcccgtat ccaagaaggg gtacgttctc
     2281 tggctggtta tgccgacatt ttttaccgca acgtcaaaag ctccggtgtc attccccaga
     2341 tctccgtcat ctacggcgct tgcgccggt
//
LOCUS       DAOQNZ010000598         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27182_length_2368_cov_3.763943, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000598 DAOQNZ010000000
VERSION     DAOQNZ010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27182_length_2368_cov_3.763943"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..2050)
                     /locus_tag="PKW73_11025"
     CDS             complement(308..2050)
                     /locus_tag="PKW73_11025"
                     /inference="COORDINATES: protein motif:HMM:NF017775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNA73865.1"
                     /translation="MKYTAIGRQTLRRLYLVGKPFWASPLKWKALALLATVLGLLFTV
                     AAVNVFVSSIAGKFTTAMQAKDVSAYHHYLLLYAAAMVAGSPVVVFYQYLRTKLALTW
                     RQWLTQHLVNRYLENRAYYKLSNLPEIDNPDERISQDVETFCNSAVGLFIAWLDAFVT
                     VCSFLGVLWLISGTLTVVALAYSLIGCLLAILIGRKLVNLQFEHTRLEADLRYTLANV
                     RRDVESIAFYNGEKSARKVIFKRIAEAIFNLELMMVTQRNLTFFTVNFNYMVALIPAA
                     IIAPLYFAGTMEFGDITKAAIAFGQVLAGMTLLVGQFTGITAFLANINRLGSFVETLD
                     ELQAHTQRPESRQGQVIAPPTQISMVEGQNLSIKQLCVDVPGIGRRLLKNLDLELGNG
                     ESVIIMGPSGSGKSSLLRAIAGLWESGSGSVSCPPRSSLMFLPQRPYVPRSTLKEAVC
                     YPRVDTCETDAEMQRAFEMVNLQGLVERAGGLKSREAREVEHEWRDILSLGEQQRLTI
                     ARLILARPRFVFLDEATSALDSENERRIYELLKKLGTTIISVGHRESLIQHHQKVLEL
                     KVQIIFYQELCQTS"
BASE COUNT          529 a          578 c          661 g          600 t
ORIGIN      
        1 ataaaaaagg aataagtcga tattattcag gcttgaagaa ccggcacaag aaattaaaac
       61 acattctaag actgatcggc aatgatataa gaatttaaat ttttcgggtg aaaaacactt
      121 aattattata aattgaataa ttattttaat gattttcaga taaaactttt acattttatt
      181 tttgttaatc cctattttat ttctattttt gtgtcgttaa tgcttccttt tgagctcact
      241 gagaagtttt acttccttta tgctgttttg actcctctag cctgggcccc ttttgaagcc
      301 atttcgatca agaggtttgg cacaactcct ggtaaaaaat tatttgaact ttcaattcca
      361 gcactttctg atgatgctgg atgaggcttt ctcggtgtcc gactgaaata atggtggtgc
      421 ccagtttctt gagcaattcg tagatgcgcc gctcgttttc actatcgagg gcgctggtgg
      481 cctcatccag aaagacaaag cggggtctgg ccaggatcag gcgggcgatg gtgaggcgct
      541 gctgttctcc cagagagaga atgtcgcgcc attcatgctc cacctcgcgg gcctcccggc
      601 ttttcagccc gccggctctt tccaccagcc cctgtaagtt gaccatctcg aaggcccgct
      661 gcatttcggc atcggtttcg caggtgtcta cgcgcgggta gcagaccgct tctttgaggg
      721 tgcttctggg cacgtagggg cgctggggca gaaacatgag gctcgagcga ggcgggcaag
      781 agacactacc tgagccgctc tcccacagtc cggcgatggc tctcaagagc gatgacttac
      841 cggagccgct cgggcccatg atgatcacgc tttcgccgtt ccccagttcc aggtcgaggt
      901 tcttcaggag ccggcgtcct ataccgggta catccacgca gagctgcttg atggataggt
      961 tttgcccctc caccatggag atctgcgtcg gcggcgcaat aacttggccc tgcctgctct
     1021 ctggcctctg ggtgtgtgcc tgcagctcat ccagggtttc cacgaaggag ccaagccggt
     1081 tgatattggc caggaaggcg gtgatgccgg tgaattgtcc caccaggagg gtcatgccgg
     1141 ccagtacctg tccgaaggcg atggcggcct tggtgatatc gccgaattcc atggtgccgg
     1201 cgaagtagag cggcgcgata atagctgccg gtatgagggc caccatgtaa ttgaagttga
     1261 cagtgaagaa ggtaaggttg cgttgcgtca ccatcatcaa ctccaggttg aaaatggctt
     1321 ccgcgatgcg tttgaagatc accttgcggg cgcttttctc gccgttatag aaggcgattg
     1381 attccacgtc gcgccgcaca ttggccaggg tgtaacgtag atccgcttcc agcctggtat
     1441 gctcaaactg cagattgacc agctttctgc cgatgagaat ggccagcaga cagccgatca
     1501 gcgagtaggc cagtgccacc accgttagtg tgcctgaaat gagccagagc actcccagga
     1561 aactgcagac ggtaacaaag gcatcgagcc aggcgatgaa gagacccacc gccgagttgc
     1621 agaaggtttc cacatcctgg gagatgcgtt cgtccgggtt gtctatttca ggcaggttgg
     1681 aaagtttgta gtaagcgcgg ttttccaggt agcggttcac caggtgttgg gtcagccact
     1741 ggcgccaggt cagggccagc ttggtgcgca ggtactggta gaaaaccacc accggtgaac
     1801 ctgccaccat ggccgcagcg tacagaagta agtagtgatg gtaggcggag acgtctttgg
     1861 cttgcatggc agtggtgaac ttaccggcaa tgctgctgac gaatacgttg acggcggcca
     1921 cggtgaagag caggcccagc actgttgcta agagtgccag ggctttccac ttcagtggtg
     1981 aagcccagaa gggcttgccg acaagatata gccgcctcag ggtttgacgc ccgattgctg
     2041 tatatttcat gacggttact ctgtttcgcc taaatcaaag cttatgcgct ctgggctatc
     2101 ctgattgaca tgccggttca ggcaggacga tagggtggag cgctggagtg gagcttagat
     2161 ttgggtcttt atttttatta gtaaagagaa gagttgatga ttctctctaa caaattctct
     2221 tctcataact caagctcggg cgagcggaat cttcgactac agtttcactt ggcaggtgct
     2281 ctcaaggggc tgctttgctt cagtgagaga aatcgcagcg tcctgtctgg actgtcaagg
     2341 gactgacatc atcagggttt tatgcagc
//
LOCUS       DAOQNZ010000599         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27256_length_2365_cov_2.887013, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000599 DAOQNZ010000000
VERSION     DAOQNZ010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27256_length_2365_cov_2.887013"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKW73_11030"
     CDS             complement(<1..291)
                     /locus_tag="PKW73_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73866.1"
                     /translation="MPRNLKRAGLLIACSLLCQFPILPGGFVSEAAFAKTAESTKEVE
                     NTQDLKDGVKFSYSSQAKKEFDGAVKTGRDFIDQYLKEHPDLEPGSLALVSDI"
     gene            complement(334..594)
                     /locus_tag="PKW73_11035"
     CDS             complement(334..594)
                     /locus_tag="PKW73_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73867.1"
                     /translation="MDIPFYIASFLLAALILVSIAESDRLVRLVKPKPKKVASGSWGV
                     YTGVSAEKSVGTVEVKPLPGRQKVIGGSITYAAGRGVKMNGG"
     gene            complement(778..945)
                     /locus_tag="PKW73_11040"
     CDS             complement(778..945)
                     /locus_tag="PKW73_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73868.1"
                     /translation="MNQNEHAKALDKRLLGLFETKAREFTRFSEENPKTAVITMLIAG
                     LYEELADIVKH"
     gene            complement(1297..1635)
                     /locus_tag="PKW73_11045"
                     /pseudo
     CDS             complement(1297..1635)
                     /locus_tag="PKW73_11045"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            1748..>2365
                     /locus_tag="PKW73_11050"
     CDS             1748..>2365
                     /locus_tag="PKW73_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73869.1"
                     /translation="MSQLSTSVQPTIKQLLATDALSEAQILCGQGNLDRPVSQVISAT
                     GHAPRQGSLVVTQSDSVSTADVALLSRLSGLILVKTSPDFGGLAMTAAASAEGLSGVS
                     SSVIGASALSEASLKRLIKRCNDAGIPLVLVPSYGEPGQVIEDVRFVFLRELKLASAR
                     LYSVMLSIALEEGLDGLVEAVSGWLNRPLVVETSDFKVLASRNMGA"
BASE COUNT          461 a          612 c          665 g          627 t
ORIGIN      
        1 gatgtccgag accagtgcca ggctgcctgg ttccaggtcc ggatgttctt tcaaatactg
       61 gtcgatgaag tcccggcctg ttttaacggc cccgtcgaat tctttcttgg cttgggagga
      121 ataactgaac ttgaccccgt ctttgaggtc ctgggtgttt tcgacttctt tggtgctttc
      181 ggccgtcttg gcgaaagccg cctcactgac gaagccgccc ggtagtatcg ggaattgaca
      241 gaggagcgag caggctatta gaagaccggc tcttttaaga ttgcgtggca aagtggcaaa
      301 tcctatatga agggactggc cggatctttt gatctagccg ccgttcattt taacgccgcg
      361 accggcagcg taggtgatac taccgccaat aactttctgg cgacccggca gtggtttgac
      421 ttccaccgtg cccaccgatt tttcggcgct tacgccggta taaacacccc aggagccgga
      481 tgccactttc ttgggtttgg gtttgaccag cctgaccagg cggtctgatt cggcaattga
      541 gacgagtata agagcggcaa gtaagaaact ggcgatgtaa aacggaatat ccacctgaga
      601 cctcctgcct gggcatgagc gcgacttatg ggctcacatt aaaccagggc ggcaggtgtt
      661 gtaaatggga aaaatactca caattcaatc ttgagaaaac aaaattgccc ccggtttaac
      721 caggggcaat ttgttttctt tccttgtttg acctctggca atcagcgcct gaagctctta
      781 atgcttgacg atatcggcaa gctcctcata aaggccggcg atgagcatag taatgacggc
      841 ggtcttgggg ttctcctcgg aaaagcgggt gaactccctg gctttggttt cgaaaagtcc
      901 aagtagtctt ttatcgaggg cttttgcgtg ctcgttttga ttcatcgggc ggtctttcta
      961 gttggcaagt tgaaataggt gaatgcttaa attttactca aaactctgaa aacctcaata
     1021 ggtcgggcag gaaaaactac tgaaaagctc aaattttatt ttcgtgacgc cacctctggt
     1081 ggcgccctat gtcttttgta tatcgcccaa atagagagag ggacaccatg ggcgcccctc
     1141 tctctctcgg agtttatgct tggaaaacta gcgacgtttc ttagctgtct ttttgctctt
     1201 gccgggtgcg gctttcttgg ccggcttggc atgaggcttg ctttccttct tcctggggtt
     1261 tggatcgctc caaccggtca gtctcttgcg accgtccacc acatccttga gttcctgtcc
     1321 ggcgcggaat gccggcactt tggcggcggg aatgcgcagc ggggaaagag tgcgggggtt
     1381 gcggccggtg cgggcctgtc tggtgcggcg ctcgaaagtg ccgaagccta ccagggtcac
     1441 cttatcgccg cgtcccatgg taccggtgat ggtgtggagg atggcggtga cggtagtgcc
     1501 aatctcggtt ttgggttgac ctgtcatttc tgcaatgtgg ctgatcagct cagctcgatt
     1561 catcttggta ttctccttga ctcgtctaat tttgaaatta ttgactccag ggcttccaaa
     1621 cggggatgtt agcactacct aatttttccc acaagggcgg ccggcgtcgg tcattaactt
     1681 tctggaaaac ccggcggcgg gccggatatt atagatatgc ttgccatggt cgctggataa
     1741 tcgataaatg agccaactgt ccacctcggt acaacctacc atcaagcagc ttctggccac
     1801 cgacgctctt tcggaggccc agatactctg tggccagggc aatctggata gaccggtcag
     1861 ccaggtgatt tcggctaccg gtcatgcccc tcgccagggg agcctcgtgg ttacccagtc
     1921 cgattccgtt tccactgccg atgtggctct gctctcgagg ctttcggggt tgattctggt
     1981 caagacctcc cctgatttcg gcggtctggc catgaccgcc gctgcctccg ccgaaggtct
     2041 gtccggcgtg tcatcttcag ttatcggtgc cagcgccctt tcggaagctt ctttaaagcg
     2101 cttgatcaag cgttgcaatg atgccggcat tccccttgtg ctggtgccga gctacggtga
     2161 acccgggcaa gtcattgaag atgtacgctt tgtctttttg cgtgagttga agctggcttc
     2221 ggccaggctc tacagtgtca tgctctccat tgccctggaa gaaggactgg atgggctcgt
     2281 ggaggctgtt tccggctggc tgaaccggcc cctggtggtg gaaacttccg acttcaaggt
     2341 gctggcttcc cgcaacatgg gggct
//
LOCUS       DAOQNZ010000600         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27398_length_2359_cov_3.628472, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000600 DAOQNZ010000000
VERSION     DAOQNZ010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27398_length_2359_cov_3.628472"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKW73_11055"
     CDS             <1..775
                     /locus_tag="PKW73_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73870.1"
                     /translation="ILNSFLIEITAKILSVKDKETGKPLVDLILDKAGQKGTGRWTSE
                     AALELSVPVPTIDGALTGRVLSSMKDERMNAARVFASVSEDLEKETAGSGGLDHPLID
                     HLEKALYAAKVCSYAQGMSLIKAGSDKYNWGVNLSETARIWRGGCIIRAELLERIRAA
                     FDRDKNLPNLLVDEDFAPFMVQSHMSMRAVIKEAIVRGIPLPAMTASLSYFDSYRAAH
                     LPQNLTQAQRDFFGAHTYERVDKPGAGFMHTEWAELIAK"
     gene            778..1650
                     /gene="recO"
                     /locus_tag="PKW73_11060"
     CDS             778..1650
                     /gene="recO"
                     /locus_tag="PKW73_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNA73871.1"
                     /translation="MATFTLNAVNIGTFPLGEADRIVVLFSRERGLHRAVAKGARKPG
                     SKMAGKSEPLTINRYLLAQGKSLDIVTQCETLESFPMLRRDLERLTYALYFAELSQVF
                     GQELSEDAANYFEKLSLAIGFMADGEKAPALQCLEFELLVLGMLGLSPELTFCVGCRE
                     TAVDRNIAAFNYELGGVVCQNCLKRLRKGENEHGQVPMVAEVQAREGRLVSVFLTPMV
                     WRQLILAKTASQASADLQNLGEAEAFASSSGGFTREDGQAASRLAQALRLMLGYIEYK
                     SGRRMRSLEVLSTL"
     gene            1769..>2359
                     /locus_tag="PKW73_11065"
     CDS             1769..>2359
                     /locus_tag="PKW73_11065"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNA73872.1"
                     /translation="MSLLHTDPELAKIIESELNRQKNCIELIASENFVSSSVLEAQGS
                     VLTNKYAEGLPGKRYYGGCEYVDGVERLAIDRVKQLFNAPHANVQPHSGAQANMAVLF
                     AMLKPGDTILGMALDQGGHLTHGSPVNMSGKWFDAKFYGVDAATGRLDYDKAEEVALA
                     VKPRLIICGASNYPRIIDFERMRSIADKVGAYLMADI"
BASE COUNT          514 a          612 c          658 g          575 t
ORIGIN      
        1 catactcaat tccttcctga ttgagataac tgccaaaatt ttgtcggtga aagacaagga
       61 aacaggcaaa ccgctggtgg acttgattct cgataaggcc ggtcagaaag gcaccggacg
      121 ctggacatcg gaagcggctc tggaactgag cgtgccggtg cctaccatcg atggtgctct
      181 tactggtcgg gttctctctt ccatgaaaga cgagcgtatg aatgccgccc gggtcttcgc
      241 ctcagtatcc gaggatctgg agaaggagac tgccggttcg ggcgggcttg atcatccgct
      301 catcgaccac ctggaaaagg cactttacgc agccaaggtc tgcagctatg cccagggtat
      361 gtccttgatc aaagctggtt ccgacaaata taactggggt gtcaacctct ccgagacggc
      421 gcgtatctgg cggggcggct gcattatccg ggccgaatta ttggagcgca ttcgggccgc
      481 tttcgatcgc gataagaatc tgcctaattt gctcgttgat gaagactttg ctcctttcat
      541 ggtgcagtcc catatgtcca tgcgggccgt aatcaaagaa gcaatcgttc ggggtattcc
      601 gctgccggcc atgaccgcct ctctttctta tttcgattct tacagggctg cccatctgcc
      661 ccagaatctc acccaggctc aaagagattt ctttggtgcc cacacttacg aaagagtgga
      721 taagccgggc gccggcttca tgcacaccga atgggccgag ctaattgcca agtaggcgtg
      781 gctaccttca cccttaatgc cgtaaatata ggtacctttc ccctgggaga agccgatcgc
      841 attgtggtgc ttttctccag ggagagagga ctgcaccggg ctgtggccaa aggggcccgc
      901 aagccaggct ccaaaatggc gggtaagtcc gagcccctca ccattaacag atacctgttg
      961 gctcagggaa aaagtctcga catagtgacg cagtgcgaaa cgctggagtc gtttcccatg
     1021 ttgcgccggg atctggagcg cctcacttac gcgctctatt ttgccgaact atcccaggtc
     1081 tttgggcagg agctatcgga ggatgccgcc aactattttg agaagctttc tctggccatt
     1141 ggtttcatgg ccgatggaga gaaagcaccg gcgcttcagt gtctggaatt tgaacttttg
     1201 gtgctgggca tgcttggact ttcgccggag ttgacctttt gtgtgggttg tcgagagact
     1261 gccgtagatc gcaacatcgc cgccttcaac tacgaactgg gtggagtggt ttgccagaac
     1321 tgcctcaagc gcttgcgcaa aggtgaaaac gagcatggac aggtgcccat ggtcgccgag
     1381 gtccaggcca gagaggggcg gctcgtcagc gtcttcctta ctcctatggt ctggcggcaa
     1441 cttattctgg ccaagaccgc cagtcaagcc agtgccgatt tgcaaaattt aggtgaggcg
     1501 gaggcttttg ccagttcctc cggcgggttt acaagagaag acgggcaggc ggccagtcgc
     1561 ctcgctcaag ccctgcgcct catgctcgga tatatcgaat ataaatcggg aaggcgtatg
     1621 cgctctctgg aggtgctttc caccctctag aaggcagtaa atatgtctag ctattgcgct
     1681 ttaggtgaga aagttttaca gatggcatta gtccttgaaa agcggctaaa atactgtatt
     1741 gtggcgctat tttcagggga gaagttttgt gtccttacta catacagatc cggaactggc
     1801 taaaatcatc gaaagcgagt tgaatcggca gaagaactgt atcgagctga ttgcctctga
     1861 gaacttcgtc tcttcttccg tgctggaagc gcaaggctct gttcttacca ataagtatgc
     1921 ggaaggtctg cccggtaagc gttactacgg tggctgcgaa tatgttgatg gcgtcgagag
     1981 attggccatt gaccgcgtca agcagctctt caacgctccc catgctaatg tccagcccca
     2041 ttccggtgcc caggccaata tggccgttct tttcgccatg ctcaagcccg gcgataccat
     2101 cctgggtatg gctctcgatc agggtgggca cttgacccat ggttctcccg tcaatatgtc
     2161 gggcaagtgg ttcgatgcca agttttacgg tgtcgatgcc gctaccggtc gccttgatta
     2221 cgacaaggcg gaagaagtgg cccttgccgt caaaccccgt ttgatcatct gtggtgccag
     2281 caactatccg cgcattatcg attttgaaag aatgcgcagt atcgccgaca aagtcggggc
     2341 ttatttgatg gccgacatc
//
LOCUS       DAOQNZ010000601         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27416_length_2358_cov_4.349544, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000601 DAOQNZ010000000
VERSION     DAOQNZ010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27416_length_2358_cov_4.349544"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PKW73_11070"
     CDS             complement(<1..864)
                     /locus_tag="PKW73_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNA73873.1"
                     /translation="MLPTFLPLSADQLKWFVEHAGLIVAAPFLAGFLIIMGLRASKTL
                     SMVTSVGAVLYGLFHSVAIFQALVTKSYPEPFFQQNIDWFVSDAFTLSVGVLVDNLAA
                     LMLIVVTGISLLVQIYTHGYMREDPGYSRFYAYLSLFTGSMLGLVVSTNLFQMYGFWE
                     LVGVCSYFLIGFWWYKESAAKACMKAFVVNRIGDFGFLIGILVFFLATKDFWQGQTLL
                     GFVGPGEANLAGVIHRAAEAHALTTESLFVISCLILMGPMAKSAQFPLHVWLPDAMEG
                     PTPISALIHAAT"
     gene            1180..>2358
                     /locus_tag="PKW73_11075"
     CDS             1180..>2358
                     /locus_tag="PKW73_11075"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNA73874.1"
                     /translation="MSHEPQHLLTVLFLVPLIGTLAIACLPETAARKLAIAFSSVMCG
                     LSVWLLSQFNLETAAEFQFVEKMPWFTQPFPVQYFVGVDGMSLSLVVLTAFITLMSTL
                     ASTSIGDNRPRLYYSMLMLLIFSVQGVFLSLDLLQFFVLYEMELVPMYFLIAIWGGPR
                     RSRAALKFLIYTFFGGILMLAGLVYMYTQMGVGAGGMGTFDMTELARLPQLLPKDVQI
                     IACLGFFLAFIIKLPSFPVHTWLPDAHVEAPTPISMLLAGLLLKMGSYGLVRICLGFF
                     PYFFIDYGPIVMALGAFNIVWAAMACLVQQDMKRIIALSSVSHMGFVLLGVASLSSAA
                     LNGAIFQMISHGVISALLFFLVGTVYERTHTRQLGEIGGGLAKQMPTLFYFWMLGTMA
                     N"
BASE COUNT          548 a          642 c          598 g          570 t
ORIGIN      
        1 ggtggcagca tggatgagag cggaaatagg tgtggggcct tccatggcat ccggcagcca
       61 gacgtgcagc gggaactgcg ccgacttggc catgggaccc atgagaatca aacaggaaat
      121 aacgaagaga gactcggtgg tgagggcatg ggcttcagca gcccggtgaa tcactccggc
      181 cagattggct tcgcctggtc cgacaaaacc aagcaaggtt tgaccctgcc agaagtcttt
      241 ggttgccagg aagaaaacca aaatcccgat caggaagccg aagtcaccga tacggttgac
      301 cacaaaggct ttcatacagg ctttggccgc cgattctttg taccaccaga aaccgatcag
      361 gaagtaagaa caaacgccga ccagttccca gaaaccatac atctggaaga ggttggtgga
      421 aacaaccaat ccgagcatgg agccggtgaa caaagaaagg taggcataaa agcgagagta
      481 gccgggatct tcccgcatat aaccgtgggt gtaaatctgc accagaaggc tgataccggt
      541 gacgacgatg agcatcaatg ccgccagatt atccaccagc acgcccaccg aaagagtaaa
      601 ggcgtcggac acgaaccaat ctatattttg ttgaaagaag ggttccggat aagacttagt
      661 aacgagtgcc tgaaaaatgg ctacggaatg gaagagtccg tagagcaccg cgccgaccga
      721 ggtgaccatg gagagcgtct tggacgccct gagccccata ataatcaaga aaccagccag
      781 aaacggcgcg gcgacaataa gcccggcatg ctcgacaaac catttgagct gatcggcact
      841 cagtggcaga aaggttggca gcaagatact tctccagatc tgggtcttcc ctcatcaaag
      901 ccgacggaca cttgactagt gacaccaccg gccgtgattg taaaactata catttttgga
      961 agttagataa gctcagtttc gagtgaaaag atttttctct tcaataattg ttcaactagc
     1021 cccttttgta gcgcaaacgg gtggcaacga tttgcacctt ccctttatac tgatggcgct
     1081 caagtgagag aagccccgaa gatatcttcc gcgaatattg cccgcgaata tcttctttat
     1141 ttaccagggt agttgctacc acgaattacg gagtcagctg tgagtcacga accacaacac
     1201 cttttgacag tgctgtttct agttccactg atcggtactt tagccatcgc ctgcttgccg
     1261 gaaacggcag ccagaaagct ggccatcgcc ttttccagtg tcatgtgcgg actctcggtc
     1321 tggcttctca gtcaattcaa tctagagact gcggcagagt ttcaatttgt cgagaaaatg
     1381 ccctggttca cccagccttt cccggtgcag tacttcgtgg gtgtagacgg catgtcgctc
     1441 tcactggtgg tcttgactgc cttcattact ctcatgtcca ctctcgcttc aacctcgatt
     1501 ggagacaatc gcccgcgtct ttactacagc atgctcatgc tccttatatt ttcggtacag
     1561 ggcgtgttct tgtctctcga cttgctgcag ttcttcgtgc tctacgaaat ggaactggta
     1621 ccgatgtact tcttgatagc catctggggc ggtcctagac gttcccgcgc cgctctcaag
     1681 ttcttgattt ataccttctt cggcggcatc ctcatgctgg ccggtctggt ctatatgtac
     1741 acccagatgg gcgtcggcgc aggcggcatg ggtaccttcg acatgaccga actggcccgt
     1801 ctgccgcaac tgctgcccaa agatgtgcag attattgcct gcctgggctt cttcctggcc
     1861 ttcatcatca aactgccctc cttccccgtg cacacctggt tgcctgatgc tcacgtggaa
     1921 gcgcccaccc ctatctccat gctcctggcc ggtttgctct tgaaaatggg ctcttacgga
     1981 cttgtcagga tatgcctggg cttctttccc tacttcttta tagactacgg cccgatagtg
     2041 atggccctgg gtgcgttcaa cattgtctgg gctgctatgg cttgcctggt acagcaagac
     2101 atgaagcgca tcatcgccct ttcatcagtc agccacatgg gctttgttct actcggtgtt
     2161 gcttcccttt cttcggctgc tttgaacggc gccatcttcc agatgatttc ccacggcgta
     2221 atatcggccc tgctcttctt cctggtcggt accgtgtatg agcgcaccca tacgaggcaa
     2281 ctgggtgaaa taggcggcgg attggccaag cagatgccta cccttttcta tttctggatg
     2341 ctcggcacca tggccaac
//
LOCUS       DAOQNZ010000602         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27443_length_2357_cov_3.607298, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000602 DAOQNZ010000000
VERSION     DAOQNZ010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27443_length_2357_cov_3.607298"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PKW73_11080"
     CDS             complement(<1..892)
                     /locus_tag="PKW73_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNA73875.1"
                     /translation="MMLVEPPIQKDKGVTISVTEQQLENAKSEFERLLEESLSFKFEQ
                     GDIVPGSVVRIERDGVLVDVGGKSEGFVPLKEVSNMPVDHIEDVCKVGESLEFYILRE
                     ENENGQLTLSLKRVAQARGWVQLENMKRNDDTIRAKISQVVKGGVVVDVYGLRGFVPA
                     SQLRVKGTTPEELIGMEIPMKILETDTKRNKLILSQRLAVQEEKAAQREKILSTLESG
                     QVVTGEVVRIADFGAFIDLGGLDGLLPISEISWERVSHPSDVLKVGQMVTTKVLKVDQ
                     EKGHISLSLKQMQPDPWKEIE"
     gene            1518..2015
                     /locus_tag="PKW73_11085"
     CDS             1518..2015
                     /locus_tag="PKW73_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73876.1"
                     /translation="MRKVRHLTLVVSAIGLSAGLATAGQVHAKPQLELLKFSGAKPAE
                     TTSTVGAAEMERTNQRVEALRKRTEQARQQLEAAKAHLRAAEAEYKAARAEKEALILR
                     NKATELASVAVGEGAQKTESPAKTEATAGRIDASELAPSETGSAGTAPAPGQPQAATL
                     DDLRQ"
     gene            complement(2050..>2357)
                     /locus_tag="PKW73_11090"
     CDS             complement(2050..>2357)
                     /locus_tag="PKW73_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNA73877.1"
                     /translation="QDLGLFHVQTLLVRLFGNPTTIADRDEVKDNFKAQLQEFTQSRG
                     QGLPCYQVLETEGPPHDPTFKVSVEIAGQIIASASGKSKKAAEQEAARLAYEKVIAS"
BASE COUNT          492 a          604 c          643 g          618 t
ORIGIN      
        1 cttcgatttc tttccaagga tcaggctgca tctgcttgag gctcaagctg atatgaccct
       61 tttcctggtc cactttgaga accttggtgg tgaccatctg accaactttg agaacgtcgg
      121 atgggtgaga aacgcgctcc caggaaattt ccgagatggg aagcagtccg tcgagaccgc
      181 cgagatcgat gaaagcaccg aagtctgcga ttctgaccac ttcgccggta acaacttgac
      241 cggattcgag agtggagagg attttttctc tctgggctgc tttctcttcc tgtacagcca
      301 gtctctggct gaggatgagc ttgttgcgtt tggtgtcggt ttccaaaatc ttcatgggga
      361 tttccatgcc gatcagttct tccggtgtgg tgcctttgac gcgcagttgg cttgccggta
      421 cgaaacctct cagtccgtag acatcgacca ctacgccgcc tttgacgacc tgtgaaattt
      481 tggcgcggat ggtgtcatcg ttgcgcttca tgttttcgag ctgtacccag cctctggctt
      541 gagcaacacg cttcagtgaa agtgtcagtt gtccgttttc attctcttct ctgagaatgt
      601 aaaactccag gctttctccg accttacaaa cgtcttcgat atggtcaacc ggcatgttgg
      661 agacttcttt gaggggtacg aaaccttccg atttgccgcc cacatcaacg agtacaccgt
      721 ctctctcgat acgcaccacg ctacccggca cgatgtcgcc ttgctcgaac ttgaaactca
      781 aagattcttc aagaagacgc tcgaattcgg attttgcgtt ttctaactgc tgttctgtca
      841 ctgaaattgt cactcccttg tccttttgaa ttggtggctc gactagcatc attgtcagta
      901 gttctccact cttgcatgaa ttgcatgtag atttggtttg ggccgtcaag ccacaggcgc
      961 cttagaaaga atctccatcc ggcggggtac gcgtggatgg tttccttgct tgcgcgactt
     1021 atcagtttac gacccatggg gcacaagcct ttcagggccg caaccttact cttgtcgacg
     1081 attttaaggt cgcttcagag gggtcgcgca ctggcgagtt ccacaatata gaccaatgta
     1141 tatccagctt tcaagagaag tgtttgaggt ttaagtgaac atttagactt gtgcttgcaa
     1201 aggctggtta ttttccgcta acaattgccc ggtcatgcct ctcactgctt ctgctcgggc
     1261 atgccgcagg tatttacgat tgccggtcaa atgtctctgt gaacgacgta ttaatgtatg
     1321 gccgccggag gtgttttcgg cttgtttccg ggtttgctca aggtggctcc aaggctcatc
     1381 aattctatct tttgagggtc tggcagacgt ttccagcggg gccacccttg actataatta
     1441 gcatcgacaa aggtaccggc attggttgtt tttatagatt cggctttgca ccgaaagtcg
     1501 ggttggggag tttacaaatg aggaaagtac gtcatctaac actggtcgtt tcggccatcg
     1561 gcttgagcgc cggactggca acggctgggc aggttcatgc caagccgcaa ctggagctct
     1621 tgaaattctc gggcgcaaag cctgcggaga ccacaagtac ggttggcgcg gctgaaatgg
     1681 agcgcaccaa tcagcgggtg gaggctcttc gcaagcgcac cgagcaggcc aggcaacagt
     1741 tggaagcggc caaggctcac ttgcgtgccg ccgaggcgga gtacaaggct gctcgcgctg
     1801 agaaggaagc tttgattttg cgcaataaag ccaccgaact ggcttcggtt gccgtcggtg
     1861 aaggcgccca gaagactgaa agcccggcta agaccgaggc cactgccggt cgtatcgatg
     1921 cttccgaact ggcgcccagt gaaacaggca gcgccggcac ggcaccggca ccgggacagc
     1981 cgcaagcagc cactctggat gacttgagac agtaaatcag ttgctcaata aatgaagggt
     2041 ggtcgtctct caggaggcga tcactttttc ataggccagt ctggctgctt cctgctcggc
     2101 ggctttcttc gacttgccgc tggcgctggc tatgatctga cctgcaatct ctacggagac
     2161 tttaaaggtc ggatcgtggg gcggaccttc ggtttccaga acctggtagc agggcagacc
     2221 ttgccctctg gactgggtga attcctgcaa ctgggctttg aagttgtctt ttacttcatc
     2281 ccgatcggca atcgtagtgg gattgccgaa gagccgcacc aatagggttt gtacgtggaa
     2341 gagtccgaga tcttgat
//
LOCUS       DAOQNZ010000603         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27472_length_2356_cov_2.902651, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000603 DAOQNZ010000000
VERSION     DAOQNZ010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27472_length_2356_cov_2.902651"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKW73_11095"
     CDS             complement(<1..840)
                     /locus_tag="PKW73_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNA73878.1"
                     /translation="MSTSKRTIRDHGQEKFNGKTVLVRVDFNVPQKEDGTVADDSRIK
                     GALSTIDFLAKAGARVVLVSHLGRPKGKPSAKYSLKPIAEHLKKLLADMGHKVVHFAE
                     DCIGEKAESTVKAMKNGEVTLLENVRFYEEEEKNDPEFARKLAALADMYVNDAFGTAH
                     RAHASTEGVTKFIKPALAGDLVAKEIEMLSSALDDATCVHPFATIIGGAKVSSKIGVL
                     ENLLAKVDILVIGGAMAFSFLKAQGLEVGKSLVEDDKLAYCRELIEKAKTRGVKLILP
                     LDVV"
     gene            complement(936..1961)
                     /gene="gap"
                     /locus_tag="PKW73_11100"
     CDS             complement(936..1961)
                     /gene="gap"
                     /locus_tag="PKW73_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNA73879.1"
                     /translation="MARVAINGFGRIGRNVFRAYIEAAPKEWEIVAINDPFQNLEQSA
                     HLLQFDSVLGEFKHEISHTQDALVVNGKSIHFSAEKDPANCPWAKLNVDMVLECSGAF
                     TDAEKAKAHVKAGAKKVLISAPAKNEDITIVLGVNHENYDPDKHHIISNASCTTNCLA
                     PLAKALDDAFGIEHGLMTTIHSYTLDQKLLDAAHSDLRRARAAAVSMIPSSTGAAKAI
                     GLVMPHLKGKLNGFAMRVPTPNVSVVDLVATLKKEVTVEEVNKVLKGASEGKLKGYLA
                     YSDKPLVSVDFCGNKISSIVDADLTMIVGERMVKVLAWYDNEWGYSNRLVELATLVAK
                     KLPVAAK"
     gene            2222..>2356
                     /locus_tag="PKW73_11105"
     CDS             2222..>2356
                     /locus_tag="PKW73_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNA73880.1"
                     /translation="MKEKVEAVLEKIRPMLMMDGGNIELVEVKDGEVFVHLVGACGMC
                     P"
BASE COUNT          478 a          552 c          681 g          645 t
ORIGIN      
        1 cactacatca agaggcaata tcaatttgac gcctctggtt ttggctttct cgataagctc
       61 gcggcagtag gccagtttgt cgtcttccac cagtgatttg ccgacttcca gaccttgcgc
      121 tttcaagaag ctgaaggcca tggctccgcc tatgaccaga atgtctactt tggccagtag
      181 attttccagc acgcctatct tgctggatac tttggcgcca ccaatgatgg tggcgaaagg
      241 atggacgcaa gtagcatcat cgagagcgga ggaaagcatt tctatttctt ttgcaacaag
      301 atcgcctgcc agagcgggct tgatgaattt ggtcacgcct tcggtggagg catgggcgcg
      361 gtgggcggta ccgaaagcgt cgttgacgta catgtcggca agggccgcca gtttcctggc
      421 gaactcgggg tcgtttttct cttcttcttc atagaagcgc acgttttcca gaagcgtcac
      481 ttcgccgttc ttcatggctt tgactgtgct ttctgctttt tcgccgatgc aatcttcggc
      541 aaagtgcacg actttgtgac ccatgtcggc gagcaatttc ttgagatgct cggcgatggg
      601 cttcagtgaa tacttggctg aaggcttgcc tttcggtctg cccaggtgtg atacgagcac
      661 aaccctggcg ccggccttgg ctagaaaatc gatggtagac aatgcgcctt tgatgcggga
      721 atcatcggcg acggtgccgt cttccttctg gggcacattg aaatcaactc tgactagaac
      781 ggtcttaccg ttgaattttt cctgaccgtg gtcgcgaatg gtgcgtttgc tggtgctcat
      841 attttggcct cgcttttggc gttgtttgac ttcagtcgaa ataagaaaag cccccgccca
      901 ttctaagatt cgggcggggg ctttctgtct gcttcttact tagcagctac cgggagcttt
      961 tttgctacga gggtagcgag ctcgaccagg cggttgctgt aaccccactc gttgtcgtac
     1021 caggccagaa ctttgaccat acgctcgccg acgatcatgg tcaggtcggc atcaacaatg
     1081 gaggatattt tgttgccgca gaagtctacg gaaaccaggg gcttgtcgct gtaagcgagg
     1141 tagcctttca gtttgccttc ggaagcgcct ttcagcactt tgtttacttc ttcgacggtg
     1201 acttctttct tgagggtggc taccaggtca acgacggaga cgttgggggt aggcacgcgc
     1261 atggcgaagc cgttcagttt gcctttcagg tggggcatta ccagaccgat ggctttagcg
     1321 gcaccggtgc tggaggggat catggaaacg gcagcggctc ttgctcttct caggtcgctg
     1381 tgagcggcat cgagcagttt ctgatcgagg gtgtaggagt ggatggtggt catcaaacca
     1441 tgttcgatac cgaaggcatc atcaagggct ttggccaggg gagccaggca gttggtggtg
     1501 cagctggcgt tggagatgat gtggtgtttg tcgggatcgt agttctcgtg gttgacaccg
     1561 agaacgatgg tgatgtcttc gtttttggca ggagcagaga tgagaacttt cttggcgccg
     1621 gctttcacgt gggctttggc tttttcagca tcggtgaagg caccggagca ttccagcacc
     1681 atgtctacgt tgagcttggc ccaggggcag ttggcaggat ctttctcagc ggagaaatga
     1741 atgcttttgc cgtttacgac gagggcgtct tgagtatggg agatttcatg tttgaattcg
     1801 cccagtactg agtcgaattg aagaaggtgg gctgactgtt ccagattctg gaagggatca
     1861 ttgatagcca cgatttccca ttctttggga gcggcttcga tgtaagcccg gaaaacgttg
     1921 cggccgatgc gaccaaagcc attaattgct actcttgcca tctgttaata actcctcgtt
     1981 gaatgagacg ggtagaagct cggcgcttgt accctgactg gcgtggactt agaggataag
     2041 gcgaaaactt ctgaaagcgc cggtttttta ttagacttta agcctttgct acggcattgt
     2101 tgcaaagttt ctcttgattt cccgctttcc aacatcctta acatcctggg aaattttgcg
     2161 ccaacctctt ggtgacgcct gtagaatagg ctgtacattc agttagtgag gaccttgtca
     2221 gatgaaggaa aaagttgaag ccgttttgga gaagatccgt cccatgctga tgatggacgg
     2281 cggcaatatc gagcttgtgg aagtcaaaga cggcgaagtt ttcgtacatc tcgtcggcgc
     2341 ctgcggcatg tgtcct
//
LOCUS       DAOQNZ010000604         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27489_length_2355_cov_4.540000, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000604 DAOQNZ010000000
VERSION     DAOQNZ010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27489_length_2355_cov_4.540000"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKW73_11110"
     CDS             <1..453
                     /locus_tag="PKW73_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73881.1"
                     /translation="GETLNGELEQNYKSSTSKKKKERALSPYEMQEQALEQSRSLYGN
                     PQGMQSQSGYGNPSPQYSQADYGNGNSGNYNNYTETSTNPGAPTYSAYASKPSKLRTA
                     LGAAGKLIGTTAAVAIPVTSIVLMNRAAARNGTMGGFNSNRFNRISGF"
     gene            651..1274
                     /locus_tag="PKW73_11115"
     CDS             651..1274
                     /locus_tag="PKW73_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73882.1"
                     /translation="MTLLIGAPMTAAADLKNLEAAYGQVVPKLKACSKSNFNWDWLTP
                     LNNLERCYLQEGNYQAAEPLARERLRILTLTFGQDGNSASAMTNLAEILLKLNKLDEA
                     EQLFKTSLSINKQESHVYGQPAPNFGCQHVSRQLTGLGHICLKRGKAQDAELFFRENY
                     DLAVKEEQERPRAIYTPQACLELGKCLQETGRPERPWRSMKRWCSYR"
     gene            1238..1432
                     /locus_tag="PKW73_11120"
     CDS             1238..1432
                     /locus_tag="PKW73_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73883.1"
                     /translation="MALYEKVVQLPVNAHSQTAKAAVLRNYADLLKKTGKAGQAGRLL
                     EEAKKLEESHKAMNRSQLDY"
     gene            complement(1439..1924)
                     /locus_tag="PKW73_11125"
     CDS             complement(1439..1924)
                     /locus_tag="PKW73_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HNA73884.1"
                     /translation="MSEAIDERFVKVRFALEPDDGGWPPVNSEGLWALKMAEHLYLID
                     NVPWFVRNLAACDVVVARPDQEGVLWFEEKHQWGGHLTLRVVLHGAIDVAEALRLFID
                     LLKPYGVSVEGIVQYGMAAVDVPPGVPLAALKALLVEGEKDGRWSFEEGLISEEWLLI
                     L"
     gene            complement(1925..>2355)
                     /locus_tag="PKW73_11130"
     CDS             complement(1925..>2355)
                     /locus_tag="PKW73_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73885.1"
                     /translation="SSQEVSALNPADPEEKARQRQDLERLSKGLNNLAALYQLQGKYQ
                     MAEELYERCLDIKLDLYGEEHLDTAVNLHNLATLHCAKRRFEKAEILFKRALEIREKL
                     LGPDDEQLLPILKNYAALLRKLEREEEAVRLEERIAALER"
BASE COUNT          639 a          669 c          573 g          474 t
ORIGIN      
        1 ggagaaactc tcaacggaga actggaacag aactacaaaa gctctacgtc aaaaaagaaa
       61 aaggaaagag cgctctcacc ctatgagatg caagaacagg ctctggaaca gtcacgctcc
      121 ctctacggca atccacaagg aatgcagagc cagagcggct atggtaatcc atctccgcag
      181 tacagccagg ccgactacgg caacggcaac agcggcaact acaacaatta caccgaaacc
      241 tccaccaatc caggcgcacc gacctatagc gcctacgctt ccaaacccag caagctgagg
      301 acggccctcg gtgccgccgg caagttaatc ggcaccaccg ccgcagtagc gattccagtc
      361 acatccattg tcttgatgaa cagagcggca gccagaaatg gtaccatggg tggtttcaat
      421 tccaatcgtt tcaatcgcat cagcggcttc taaaagagaa caaacccaaa attgcaccta
      481 aaccacaagg actattcagg tccgaaacag cctgccgctt gaccgactcc taattccggg
      541 agtcggtcag tcaggcataa tatagattgg agaataaagg cacagaaatg aacggcccat
      601 accggaaaaa atcgccaggc ttgttaactg ttctggcatt gtcattatcg gtgactctgt
      661 taataggtgc gcccatgacg gcagcggctg atttgaaaaa cctggaagcg gcttacggcc
      721 aggtagtgcc gaagctgaaa gcctgcagca agagcaattt caactgggac tggctcaccc
      781 ccctcaataa tctggaaaga tgctatttgc aggaaggcaa ttatcaggcc gccgagcctc
      841 tggcccggga acgcttgcgc attctcacct tgacctttgg tcaagacggc aacagcgcat
      901 cagccatgac caatctggca gagattcttc tcaaattaaa caaactggat gaagcggagc
      961 aattgttcaa aacctccctc tccataaaca agcaagagtc tcatgtctac ggtcaacctg
     1021 cccccaactt cggctgtcag catgtgagca ggcaactgac cgggctgggg catatttgct
     1081 tgaagcgagg taaggctcaa gatgccgagc ttttcttcag ggaaaactat gatctggccg
     1141 tcaaggaaga acaagagaga ccgagagcca tttacacacc acaagcctgc ctggaactgg
     1201 gcaagtgcct gcaagaaaca ggcagaccgg agaggccatg gcgctctatg aaaaggtggt
     1261 gcagttaccg gtgaatgccc acagccagac ggctaaagcg gcggtattga gaaactacgc
     1321 agatctgttg aaaaagaccg gcaaagccgg tcaggccggt cggcttttag aagaagctaa
     1381 aaaactggaa gagagccaca aagccatgaa ccgctcgcaa ttggactact gacactgctc
     1441 aaagaatcag tagccactcc tcactgatca ggccttcctc gaagctccag cgcccgtcct
     1501 tctcgccttc cacaaggagc gctttcaagg ctgccagagg cacacctggg ggcacgtcaa
     1561 cagcggccat gccgtattga acgattcctt ctacggacac gccatacggc ttgagcaaat
     1621 caatgaacaa gcggagggct tctgcgacgt caatggcacc atgcagcacc accctcaaag
     1681 tcagatgccc gccccattga tgcttttctt caaaccagag caccccttct tgatcaggtc
     1741 tggccaccac cacatcgcaa gcggcaagat tgcgaacaaa ccagggcacg ttgtcgatca
     1801 agtagagatg ctcggccatc tttaaggccc agagcccttc gctgttcacg ggaggccaac
     1861 cgccgtcgtc cggctcgagg gcaaatctga cctttacgaa gcgctcgtct atggcctcag
     1921 acatctaccg ctccagagcg gcgatgcgtt cttccaggcg gaccgcttct tcttcccttt
     1981 ccagtttgcg caaaagagcc gcataatttt tcaatatagg taaaagctgc tcatcatcag
     2041 ggccaagcag tttctcccga atctcaagag cccgcttgaa gagtatttcg gccttttcaa
     2101 aacgacgctt ggcgcaatgt aaggtagcca ggttgtgaag attcacagcc gtatccaggt
     2161 gttcttcacc atacagatca agctttatat cgagacagcg ctcgtaaagt tcttccgcca
     2221 tctgatactt gccctgcaac tgatagagcg ccgccaggtt gttcaaacct ttgctgaggc
     2281 gttccagatc ctgacgctgt ctcgcttttt cttccgggtc ggctggattg agagcactca
     2341 cctcctggct cgatt
//
LOCUS       DAOQNZ010000605         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27521_length_2354_cov_3.411918, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000605 DAOQNZ010000000
VERSION     DAOQNZ010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27521_length_2354_cov_3.411918"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PKW73_11135"
     CDS             complement(<1..1631)
                     /locus_tag="PKW73_11135"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase catalytic subunit"
                     /protein_id="HNA73886.1"
                     /translation="MFRGKLTLTVISILLALTALMAVSMSTLHLSLSFLLDLGCSLFS
                     CGDFTPHAPQAYLALFCALTFLVSRTFSRDFAPARLLVSFLLVCLGLRYLLWRVLSLA
                     MQDDSTTIYVQVFYLLIELAVFLNSLLFIIITAWPTNRSRQADELQVAVDSGLYLPRV
                     DILIPSYNEDGEMLRRSICAAQAIDYPPHLVRVFLLDDSRRDHIERLCSELGCNYVRR
                     PDNTHHKAGNVNHALRFASPSIDADLLVFFDADFMPVTTFLRRTVGFFLDGQVAMVQT
                     PQLFFSPDAPRQNLGVETRSTNEEDLFFQVIQPGRDRFNATMCHGTCFVIRRSILEEI
                     GGLPTDSLCEDWGTSILVQSRGYKIPYLNERLSCGASAVTVANFVKQRLRWAQGTWQM
                     LLTPLSPLRIRGLSFMQRLVHMAGVLHWCMSLCTVATILLPVIFFTTGTGPFRVTIEE
                     AVAYWLPYYAANGLAFAWLTRGYRAFFWSDIYGPLLAVPVFVSFVRFLWKPFGATFQV
                     TQKSGAKGGVAVCWSVLWPILAILSSYAAAALYFA"
BASE COUNT          705 a          626 c          579 g          444 t
ORIGIN      
        1 aaggcaaaat ataatgcggc cgcggcataa ctactcagaa tcgccagaat cggccagagc
       61 acagaccagc agacggccac accacctttg gcgccacttt tctgggtcac ctgaaaggta
      121 gcgccaaagg gcttccataa gaacctgaca aaacttacaa agacaggcac cgccagcaaa
      181 ggtccgtaaa tgtccgacca gaagaaagcc ctataaccac gggtcagcca ggcaaaagca
      241 agaccattgg cagcatagta aggtagccag taggccaccg cctcttcaat cgtgactcga
      301 aacggaccgg taccggtggt gaagaagatg acgggcaaca gaatggtggc taccgtgcac
      361 aaactcatac accagtgcag aacaccggcc atatgcacaa gacgttgcat gaaactcaaa
      421 cctctgattc gcaagggact gagaggggta agcagcatct gccaggtacc ttgagcccag
      481 cgcaaccgct gcttaacaaa attggccacg gtcacggcac tggctccaca actaagacgc
      541 tcattcaaat agggaatttt atagcccctg gactgcacca gaatcgacgt gccccagtcc
      601 tcgcaaagag aatcggttgg cagcccgccg atctcctcca gaatagaacg cctgattaca
      661 aaacaggttc catggcacat ggtggcattg aacctgtctc gtccaggttg aataacttga
      721 aaaaacaagt cctcctcatt agtgctccgg gtttctacac caagattctg cctcggcgca
      781 tccggagaga aaaacaactg tggcgtctgc accatggcca cctgcccgtc cagaaagaag
      841 cctacggtgc gccgcagaaa ggtggtaaca ggcataaagt cagcatcgaa gaagaccagc
      901 aagtcggcat ctatggacgg actggcaaac cttagcgcat ggttcacatt gccggcctta
      961 tggtgcgtat tgtccggtcg acgaacatag ttgcagccca gttccgaaca gagccgctca
     1021 atatgatcac ggcgagaatc atcaaggagg aaaacccgca ctaggtgcgg cggatagtca
     1081 atcgcctgag cggcacaaat cgaacgccgc agcatttcac cgtcttcatt gtaggaaggt
     1141 atgagaatgt cgactctagg caagtagaga ccactatcaa ccgcgacctg cagttcatcg
     1201 gcctggcggc tgcgattggt aggccaggct gtaatgatga tgaagagcag actattgaga
     1261 aagaccgcca gttcaatgag cagataaaac acctgcacat aaatcgtagt ggaatcgtcc
     1321 tgcatagcca gactcaagac tcgccagagc aagtaacgca gtccaagaca gactagcaag
     1381 aaggaaacca ataagcgagc aggcgcgaag tcccgactaa aagttcggct gacaagaaag
     1441 gttagcgcac agaagagcgc aagataggcc tggggggcat ggggagtgaa atctccacag
     1501 gaaaacaggg agcagccaag gtccaacaag aaagagagcg aaaggtgcaa tgtcgacatg
     1561 gacactgcca tcagcgccgt tagcgctagc agtatagaaa ttaccgtcag cgtcaattta
     1621 cctctgaaca taatgtcatc ctcgcgtagg ttaggtcaac atcgagagtg ctgcttgaaa
     1681 taacgggcaa aacttcaaag aagttgattc cgccattggg ggtgaaaacc ctgctcatca
     1741 actacgtgaa ctgatagggt tcgcctgtgg tactgctgca cttttttgcc tgtctgttcg
     1801 ggctcccgag gtctaaggaa aaacgaggta atacccggac tgtagcgcat cggctactta
     1861 aatgagacgc cgactctgta aagattcaag cctgcaagca ggcttaacaa accgtcaaca
     1921 aatgcaccgc taccggtggc acactctcat tccatcagca cttaaaggac gggtcattag
     1981 ctttacaaac actcctgacc acctgtaaag ttgccaactc cagcaagcgc aactagcacc
     2041 acccactttt cctgaaagtc ctttgcacaa ggaaggccaa ctagcacaaa ccgcgccaac
     2101 ccaagaggat gcaagcgcct aaacaagagc gcttacagaa agtcggcaaa gtttcaagct
     2161 taggcaagaa agccgttacg tccaaatcta cggctagcac aactatctat agccgcgcca
     2221 acctcatggt cataggtgct ctgtgacttt ggtcagttct cagatcaatt gccaggatgt
     2281 aatctcttgc aagcagattt gacacgaggt cagtaaacac attgctgcca tccaagttct
     2341 ttcaaagcaa tctt
//
LOCUS       DAOQNZ010000606         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27522_length_2354_cov_3.278817, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000606 DAOQNZ010000000
VERSION     DAOQNZ010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27522_length_2354_cov_3.278817"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1311
                     /locus_tag="PKW73_11140"
     CDS             286..1311
                     /locus_tag="PKW73_11140"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNA73887.1"
                     /translation="MNCFTRLYKRVVVLTGAGVSTASGLPTYRGQGGLWSDPETARFT
                     TPRAFAKDPDGAWKFWSTLREHCLKAEPNPGHLALSDWGRKLNSWEDRSFTLITQNVD
                     GLHERAGSHDAITMHGSILHSRCTNDTCDLAVFEDRELFFKGAPRCPECKSVLRPDVV
                     LFDEAIPAKAEWLVRQALRNCDLFIAVGTSGTVSPAAGFVNSAKYVGATTLLVNLEPM
                     ESVNKAFDLELLGKAESLLPRLEVEFFYKQVMFSGDLPPASRDREEYAHQRFNMALSP
                     EEQAAREAHTELLGGLDSVRYADPQFDAWVHRYYAVVCAQQYLNECRRKYLTEAELEA
                     VSSDDGL"
     gene            1636..>2354
                     /locus_tag="PKW73_11145"
     CDS             1636..>2354
                     /locus_tag="PKW73_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNA73888.1"
                     /translation="MNEKHFTKEQIQYILKEAESATNIYEICLKHDISENTLYEWLAE
                     YTPDKVQKTGFNLRQFMHEIFNGGITRRELLYAGATGIAGFAAGNIFAMVGKVRMPEV
                     LGHKIEVEDASKYKQSSEELGDYLYLTPQKLGGGTHVVDFKQGITLASISYWNYGDSC
                     PISHHLSAYPSSNPRKGFEFVNSTQGGKNVTMYSITTEIEERGLLDTWGQGNHIYRVE
                     FDGTKMELKEDIAKTTGIGLGV"
BASE COUNT          644 a          646 c          601 g          463 t
ORIGIN      
        1 cttcccgaag atggcgaaga tttctgcgaa gctcgcgtca tcgatgcaga agacgtagtg
       61 ctatgcgtgc gacgcatgta ttcgttctaa attccgaata aattcccagt accacggcac
      121 cgacaccgca gccaaccggc cagggagccg gatcaggcat tagtggtctt ccctttcttc
      181 ccgactactg tttcaaacca gaaaatgcag cggggcgaaa tcgggaatat tacggccagt
      241 catagtctac aatctaatta ccgccgcaag gagactgaaa tcagtatgaa ctgcttcacc
      301 agactctaca aaagagtggt tgtcctgacc ggagcaggag tatcaacggc ctcgggcttg
      361 ccaacctaca gaggtcaggg agggctctgg tccgaccctg aaacggctcg ctttaccact
      421 ccccgggcct ttgctaaaga cccggacggt gcctggaaat tctggagcac cttgcgcgag
      481 cactgcctta aagccgaacc aaatccgggt cacctggccc tttccgactg gggtcgcaaa
      541 cttaactcct gggaagacag gtcatttacc ctcatcactc aaaatgtcga tggcttacat
      601 gagcgagccg gcagccatga cgcaatcaca atgcatgggt cgatactgca cagcaggtgc
      661 acaaacgata cttgcgatct tgcagtcttt gaagacagag aactgttttt caagggggcg
      721 ccgcgctgcc cggaatgcaa gagtgtgctg agacccgatg tggtgctctt cgatgaagcc
      781 ataccggcaa aagcagagtg gttggtacgg caagccctga gaaactgcga cctgtttatt
      841 gcagtgggta cctcaggaac ggtgagccca gccgccggct ttgtcaacag tgccaagtac
      901 gtcggcgcca caaccttgct tgtaaatctg gagcccatgg aatcggtaaa caaagctttc
      961 gacctggagc ttctgggaaa agcggagtca ctactaccgc gcctggaagt ggagttcttc
     1021 tacaaacagg tgatgttttc aggcgacctg ccacctgcga gccgcgaccg agaagagtac
     1081 gcacaccagc gcttcaatat ggctctcagt ccggaagagc aggcagcccg ggaagcccat
     1141 acggaactgt tgggtggtct cgatagcgtc aggtatgccg acccccagtt tgatgcctgg
     1201 gtgcaccgct attacgcagt ggtctgcgcc cagcagtacc tcaacgaatg ccgccgcaag
     1261 tatctgacgg aagcagagct tgaagcggtc tcaagcgatg acggcttgtg attgaccacc
     1321 cagatatttg ccgcacaaat gactgaacca tcagtaactg cgacataaat agctgcaact
     1381 taaataaccg cgacagcatt aactgcgaca taaatagctg caacttaaat aactgccacg
     1441 ccggcaaggc aacacaaagc aaagcaaaac agcagcggca accaacgccg aggcggcagc
     1501 ctaccggccg ctgtaggccg agcccttcca ttaaggtatc cttaggcgat taaattgaca
     1561 gggcaaaagc tcgcagtcaa caaccgacca taccgacacc aaccgaccct accgacaaaa
     1621 agagatggcg ctcaaatgaa cgaaaagcat tttacgaaag agcaaattca gtacattctc
     1681 aaggaagcgg aatcggcaac gaacatctac gagatttgct tgaagcatga catctcggaa
     1741 aatactttgt acgagtggct ggccgaatac actcccgaca aagtgcaaaa aaccggtttc
     1801 aatctgcgtc agtttatgca tgaaatcttc aatggcggca tcacccgcag agagctactt
     1861 tatgcaggag ccacaggtat agccggtttt gccgccggca atatcttcgc catggtcggc
     1921 aaagtaagaa tgccggaagt gctcggtcac aaaatagagg tggaagacgc atccaagtac
     1981 aagcaatcca gtgaagagct gggcgactac ctctacctta cgccccagaa gctgggtggc
     2041 ggcacccacg tcgtagactt caagcagggc atcaccctgg cttccatcag ctactggaat
     2101 tatggcgact cctgccccat ctcgcaccat ctgtcagcct atccatccag caatccccgc
     2161 aaaggttttg aattcgtcaa ttccacccag ggcggcaaaa atgtcaccat gtacagcatt
     2221 accaccgaaa tcgaagagcg cggcttgctc gatacatggg ggcagggtaa tcacatttac
     2281 cgggtcgaat tcgacggcac aaaaatggaa ttgaaggaag acattgccaa aacaaccggt
     2341 atcgggctcg gagt
//
LOCUS       DAOQNZ010000607         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27662_length_2348_cov_2.952900, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000607 DAOQNZ010000000
VERSION     DAOQNZ010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27662_length_2348_cov_2.952900"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKW73_11150"
     CDS             <1..687
                     /locus_tag="PKW73_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA73889.1"
                     /translation="ILIPVFNEEKPLVTVLDMVANADTLGLTKELIIVDDGSSDRTRE
                     ILAGLDAEKYHAKIYYHDKNQGKGAALRTAQGHAEGDLIMIQDADLEYDPKEYPELLK
                     PILEGRADVVYGSRLCGGKPIRAFKITHLLGNKFLSLVTNILYNATLTDMETCYKVFK
                     KEVFKKVKIKCDRFDFEPEITAKVLKQGVRLYELPISYYGRDYDEGKKITWKDGIWAI
                     CALIRFRFSD"
     gene            790..1368
                     /locus_tag="PKW73_11155"
     CDS             790..1368
                     /locus_tag="PKW73_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73890.1"
                     /translation="MNKFSLLRSLSMRPAKLVLCLIVSLAWFSDPAQAQSYNYRIGDT
                     RVQAGHNGLAGHSSTYITSGTLSRSAQAAVLPMPPQIGGQGVCGMTPGVSANPMGLQS
                     ARMGSTVGMAGDYMRSDLNPNWSITQRQQAQQGQYRQQQYQIRQPVLRQAPPPNYTTY
                     GTATGQNNYHTSGVPASGGASYGSGSSGYKGY"
     gene            1432..>2348
                     /locus_tag="PKW73_11160"
     CDS             1432..>2348
                     /locus_tag="PKW73_11160"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNA73891.1"
                     /translation="MNSQVKRVVVIGGAGAMGHVVVRDLLDNPQIEVVIADYDRDKAD
                     ALADTLNSKLKTTRVRGDFTDITNKPVMVKGLKNAACVINSTPYYHNVNVMEAALEAG
                     CHYVDLGGLFHVSKEQLKLHDRFVEKGLTAVIGMGAAPGMTNIMAAKAQENMESVESI
                     DIYVGSIDNTKSSHPFLPPYSIETLIDEYTMPPMVFDGDYKEMPPLSGAVIVDFPEPV
                     GKQEAIYTLHSEVLTLPQTYMDKGIKSCTFRLGLPLEFHEKIKFLMSLGFGSREKLST
                     KEGDFTPRKILAAMLSSFPTPESEPDDCEV"
BASE COUNT          615 a          675 c          581 g          477 t
ORIGIN      
        1 atcctcattc ccgtattcaa cgaagaaaag cccctggtca cagttctgga catggtggcc
       61 aacgccgaca ccctgggact gaccaaagag ctaataatcg ttgacgacgg ctcttccgac
      121 cgcaccagag aaatattggc cggtctcgat gctgaaaagt atcacgccaa aatttactac
      181 catgacaaga accagggcaa gggtgcggca ctgcgcacgg cgcagggtca cgccgaaggc
      241 gacctgatta tgattcaaga cgccgacctg gaatacgacc cgaaagaata tccggagctt
      301 ctcaaaccaa ttctggaagg acgcgccgat gtggtctacg gctcccggct ttgcggcggt
      361 aagccgattc gagccttcaa gatcacgcac ttattgggca ataaattcct cagtctggtc
      421 acaaacattc tctataacgc caccctgacg gacatggaga cctgctacaa agtattcaag
      481 aaggaagtct tcaaaaaggt caaaatcaag tgcgaccgct tcgacttcga gcctgaaatt
      541 acggcaaaag tcttgaaaca gggggtgcgc ctctatgaac tgcccatttc ctattacggt
      601 cgcgattacg atgagggcaa gaaaataacc tggaaagacg gtatatgggc aatttgcgcc
      661 ttgattagat tccgtttcag cgactaaaac tgttgcctag caacttgtgg aagggcgaac
      721 taaaggttat cctccgttaa tgaaatattt ggtgcacccc accccttacc aagttgaacc
      781 ccgatgaaca tgaataaatt ttctttgctg cgcagcctta gcatgcgccc cgccaaatta
      841 gtgttatgcc tgattgtaag cctggcctgg ttttcagacc ccgcacaagc ccagagctat
      901 aactatcgaa ttggagacac ccgggttcag gccggtcaca acgggctggc cggtcattcc
      961 agtacttata taaccagcgg caccttgagc cgttcggccc aggccgccgt cctgcccatg
     1021 ccgccccaga ttggcggtca gggagtgtgc ggcatgaccc ccggcgtatc agccaatccc
     1081 atgggtctgc agagcgcccg tatgggttcc acagtgggta tggccggcga ttacatgcgt
     1141 tccgacctca atcccaactg gtccataacc cagagacagc aagcccagca gggacagtac
     1201 cgccagcaac agtaccagat aagacagccg gtgctgcgcc aggcgccgcc gcccaattac
     1261 accacctacg gcacggccac cgggcaaaac aactatcaca cctccggcgt acccgctagc
     1321 ggcggcgcca gctacggttc cggttccagc ggctacaaag gctactaaag cgcagaggcc
     1381 ttcctgctag acttagcccg ttaatgagtg cactctcggg caagttttca aatgaatagt
     1441 caagtgaaaa gagttgttgt gattggcggc gccggcgcca tgggtcacgt agtggttcgc
     1501 gacctcctgg ataaccctca aatcgaagta gtcattgccg attacgaccg ggacaaggca
     1561 gatgccctgg ctgataccct caactcaaag ctgaaaacca caagggtaag aggcgatttt
     1621 accgatatta ccaacaagcc ggtcatggtg aaaggcttga aaaacgcagc ctgcgtcatc
     1681 aattccaccc cctactacca taacgtcaat gtcatggaag ccgcccttga ggccggctgc
     1741 cattatgtgg acctgggcgg gctctttcat gtcagcaaag aacaactcaa gctgcacgac
     1801 cgcttcgtcg agaagggtct gacggctgtt atcggcatgg gtgctgcacc gggcatgacc
     1861 aatataatgg ccgcaaaagc acaggaaaac atggaaagcg tagagtccat agacatttat
     1921 gtaggttcca tagacaacac caaaagcagc catcccttcc tgccgcccta ttccatcgag
     1981 actctcatcg atgaatacac catgcccccc atggtcttcg acggcgacta caaagaaatg
     2041 ccgcccctct caggggccgt aatcgtagac tttcccgagc cggtgggcaa gcaagaagcc
     2101 atctacaccc tgcactcgga agttctcacc ttacctcaaa cctatatgga taagggcatc
     2161 aagagctgca ctttcagact gggactgcct ctggagttcc acgaaaaaat caagtttctc
     2221 atgtcgcttg gtttcggctc ccgggaaaaa ctcagcacca aagaaggtga ctttaccccc
     2281 cgcaagattc tggccgccat gttaagcagt ttcccgacac ctgagagtga gcccgacgac
     2341 tgcgaagt
//
LOCUS       DAOQNZ010000608         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27669_length_2347_cov_4.662304, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000608 DAOQNZ010000000
VERSION     DAOQNZ010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27669_length_2347_cov_4.662304"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PKW73_11165"
     CDS             complement(<1..569)
                     /locus_tag="PKW73_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73892.1"
                     /translation="MEKPATTASKARLLLNAEPFGFGPTAAIAECFLHLRPLFQTIGY
                     LGKGHTLDLQKPLPYDRIHDLEREENPEAVFADYDIFFTALDFEMAGKALAHGLTVII
                     YDPLTWYWREIPAVVKDCHLYLSQNFLGVQERLDACRQAFPAATVAVPPLVPAQLSET
                     AQAEVAAKRSLTGKPLMLLNLGGLANPYW"
     gene            complement(541..1686)
                     /locus_tag="PKW73_11170"
     CDS             complement(541..1686)
                     /locus_tag="PKW73_11170"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNA73893.1"
                     /translation="MYIIDDRIRQLVQEIENCSPQSDSLMAELVRALQVNCAKPEVQA
                     RYKAYRQTGHSSALDLPPMPLDENGFLKGFDPLTQEAEFLQFWQTYGIVVSKSVVSAA
                     LCERVIARMHEVCLQLSDGKFSLDSSTPDDQAHMPVDATGVALMTRGFFELYHDDAIA
                     ALRQALRVYLHHVLLWGRADLWTTFDRLGIKRPAQEGAEALPLHVDQNPNVHPHFRTV
                     QGVLALRHCPAERGTFVGVPGSPRHFHAYGEMAKNQGEYVELDPNHPLYEALSQAAQL
                     CPLAQGDLISWDSRTTHANSANLSNETRYVAYICAGPAAEDNQEAVAARAEALQTGIG
                     KNVRDALMHASKPPRFTDPERLQAVRQVETLTFLGNLLYGKTRYDSL"
     gene            2069..>2347
                     /locus_tag="PKW73_11175"
     CDS             2069..>2347
                     /locus_tag="PKW73_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73894.1"
                     /translation="MQKGLEKGLKAKKVPKFRRKFGRSEENYLILKIRIHSGSVWTKK
                     VKKGRKEVNFLSSSEELPGEYDRPLVSDRSVGRYQVRGDDGSGEHAEQH"
BASE COUNT          484 a          592 c          766 g          505 t
ORIGIN      
        1 ctccagtagg gattggccag accgcccagg ttgagcaaca taagcggttt tccggtgagt
       61 gatctttttg ctgcaacctc ggcttgggcg gtctcgctca gctgggccgg caccaggggc
      121 ggtactgcca ccgtggcggc ggggaaggct tgacggcagg cgtccagtct ctcctgcacg
      181 cccagaaagt tctggctcag gtagagatgg cagtctttca ccacagcggg aatttcacgc
      241 cagtaccagg tgagcgggtc gtagatgatg accgtgagcc catgggcgag agccttcccg
      301 gccatctcga aatcaagggc cgtgaagaag atgtcgtagt cggcaaagac tgcttccggg
      361 ttttcctccc tctccaggtc gtggatgcgg tcgtagggca ggggcttctg caggtcgagg
      421 gtgtgaccct tgcccaggta gccgatggtc tggaagagcg ggcgcagatg taggaaacat
      481 tcggcaatag ccgcagtggg gccgaagccg aagggctcgg cattgagcag aagtcttgcc
      541 ttagaggctg tcgtagcggg tttttccata gagcaggttc cccaagaagg tgagggtttc
      601 aacctggcgc acggcttgga gccgctccgg gtcggtaaaa cgaggcggct tggaagcgtg
      661 catgagagcg tcgcgcacgt tcttgccgat gccggtttgc agggcttcgg cgcgagcggc
      721 gacggcctcc tggttgtctt cggcggcggg accggcgcag atgtaagcca cgtagcgcgt
      781 ctcattggag aggttggcgc tattggcgtg ggtggtgcgg ctatcccatg aaatcaggtc
      841 gccctgggca agcgggcaga gctgtgcagc ctggctcagc gcctcgtaaa gcgggtggtt
      901 ggggtccagt tccacatact cgccctggtt tttggccatc tctccgtagg catgaaagtg
      961 cctgggggag ccgggtacac ccacaaaggt gccgcgctcg gccgggcagt ggcgcagtgc
     1021 cagcacgccc tggacggtgc ggaaatgggg gtgcacattg ggattctggt ctacgtgcag
     1081 gggcagagct tcggctcctt cctgagcggg gcgcttgatg ccgaggcggt cgaaggtggt
     1141 ccagagatcg gcgcgtcccc agagaagtac gtgatgcaga tagaccctga gcgcttgtct
     1201 gagagcagcg atggcgtcat cgtgatagag ctcgaagaag ccccgggtca tgagcgccac
     1261 accggttgca tcgacgggca tgtgtgcctg gtcatcaggc gtgctgctgt ccagactgaa
     1321 cttgccgtcg gaaagctgca ggcagacttc gtgcatgcgg gcgatgacac gctcgcagag
     1381 agccgcactg accacgcttt tgcttacgac gatgccgtag gtctgccaga actgaaggaa
     1441 ttctgcttcc tgggtgagtg ggtcaaagcc tttcaggaag ccattctcgt ccagtggcat
     1501 gggcggcagg tcgagggcgg aggagtgacc ggtctgtctg taggctttgt agcgagcctg
     1561 cacttccggc ttggcacagt tgacctgcaa ggctcgaacc agctcagcca tgaggctgtc
     1621 gctctggggt gaacagtttt caatttcttg aaccagttgc ctgattcggt cgtcgattat
     1681 gtacatttag gtctccgttg tttacgtgca gaaagattga gaagccgccc tgggcaccgc
     1741 tctcgattga gaaccggcgg tgggcgaatg cttctcggtc aactacggca agctgctttt
     1801 gacttgctgt gccgtttaga tttggtgcga gactatgttt aagacttctt caaaaagaac
     1861 gaaaagagat atacctctgc ctaacaaaga gcggcgcgat aactcaattt agatagttgt
     1921 caggtgaatg tgtcggcgct catctgtacc gtagcgctgc tctctcaggg ctcctccgct
     1981 cggggaagaa gtgccgtttt atgcgggttt ggcgaccttt gttggagaca tatttcttaa
     2041 agtgccaaaa tagcaaaaag tacttaaagt gcaaaaaggg ctggaaaaag gccttaaagc
     2101 taaaaaagtc ccgaagttcc ggcgaaaatt tggcaggtct gaagaaaact atctcatcct
     2161 gaagattcgc attcactccg gcagcgtgtg gaccaaaaag gtaaaaaagg gaagaaagga
     2221 agtcaatttc ctttcttcct cagaagagct cccgggggag tatgaccgac cgctggtctc
     2281 agaccgcagt gtcggtcggt atcaagttag gggcgacgac ggctcaggtg agcacgccga
     2341 gcagcac
//
LOCUS       DAOQNZ010000609         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27682_length_2347_cov_3.264398, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000609 DAOQNZ010000000
VERSION     DAOQNZ010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27682_length_2347_cov_3.264398"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKW73_11180"
     CDS             <1..235
                     /locus_tag="PKW73_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73895.1"
                     /translation="NRAMTDYLVRHLDLPEPDAARAFVSQLGDAVTFYKIGLELFMAG
                     GYFELLEWLTGKGKKADKVEVKDGAAPAEGAKN"
     gene            257..>2347
                     /locus_tag="PKW73_11185"
     CDS             257..>2347
                     /locus_tag="PKW73_11185"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNA73896.1"
                     /translation="MLSKTGIFPGFLALSLLLISGVSSPVPAKNGTSGAAPPASSITA
                     TGADPKMGIEAFKLKNGLKVLLVERAAAPVVVVNMIYHVGSRNEAVGYTGSTHFLEHL
                     MFKGTAAHDPLKGTGLDDLLKGVGGINNATTSYDRTNYYEVVPRESVDLALQLEADRM
                     RHLLLRKADRDAEMTVVRNELERGEDEPSELLSNMAFATAFKEHPYHHPVIGWRSDVE
                     GVPLSRLRRFYDDFYWPNNATLVVTGDFRKKELLKKIEAAFGGIPSSPKPFPAVYTKE
                     PAQEGERRFLVSRGKELPRVLVAFHTPKGIDRATAPLDVVASVLGDSSRKSSRLYKSL
                     IETGLCSECSASNYTLLDPGLFFVQATVQPGQNPRQVEAVILAELNRINESGITETEL
                     LRARQSIVKRFKLSLSDPMNLTQSLTEGIAVGSWQWWCAYPDSVAKVSLAEAKDFAGR
                     TFTENNRTVGYYLPKPTSESSTASTGYYQFPAPKPVELPALAGGELEQTQGSFPFAGL
                     LTAGSGKSEISPRVKKVVLENGLTVLLYPIARSGTVAISGKIRAGDYFARAPHTERND
                     LLARLLSFGTKTYSKQELAQKLESMGASLDFDAETFFHNFETEVTTQDLPLMVKLLAS
                     CMIEPSLRNEDLKEVSQIYAAQLKEESTQTASLAWNKVLGTLYKKDSVYHADGFDEQI
                     KAVSDTSIEEIRAHH"
BASE COUNT          539 a          681 c          629 g          498 t
ORIGIN      
        1 caaccgggcc atgacggact atctggtccg ccacctggac cttcccgagc cggatgccgc
       61 ccgggccttc gtctctcagc tgggcgacgc cgtcaccttc tacaagatcg gcctggaact
      121 cttcatggcc ggcggctatt tcgaactgct ggaatggctg acgggcaaag gcaagaaggc
      181 cgacaaagtg gaagtaaaag acggggcggc cccggctgaa ggcgccaaga actaagaaag
      241 ctgaaaagga cccccaatgt taagtaaaac agggatattc cccgggtttt tagccctgag
      301 cctcttgctt atatcggggg tctcttcacc cgttccggcc aagaacggca ccagtggcgc
      361 cgcccctcct gcctcgtcca tcaccgccac cggtgccgac cctaagatgg gcatcgaagc
      421 tttcaagctc aagaacggct tgaaggtgct cctggtggaa agagctgccg caccagtggt
      481 ggtggtgaac atgatttacc atgtcggttc acgtaatgag gcggtgggct acacaggctc
      541 cactcatttt cttgagcatc tgatgttcaa aggtaccgcc gcccatgatc ctcttaaggg
      601 caccgggctt gatgatttgc tgaaaggggt gggtggtatc aacaatgcca ccacctccta
      661 cgatcgcacc aattattatg aagtggtgcc cagggagtct gtagatctgg ccctgcaact
      721 ggaagccgat cgtatgcgcc atctgctctt gcgcaaggct gatcgcgacg ccgagatgac
      781 cgtggtgcgc aatgagttgg aacggggcga agacgaacct tcggaattat tgagcaatat
      841 ggcttttgcc actgccttca aggaacaccc ctatcaccat cccgtcatcg gctggcgctc
      901 cgacgtggag ggtgttcctc tgagccgatt gcgccgcttc tacgatgatt tctactggcc
      961 gaacaacgcc accctggtgg tgaccggtga tttccggaag aaggagctac tcaagaaaat
     1021 tgaagctgct ttcggcggca ttccttccag tcccaaacct tttcccgccg tctatacaaa
     1081 agaaccggcc caggaaggag agcggcgctt tctcgttagc cgcggcaaag aactgccacg
     1141 ggtactggtg gcatttcaca ctcctaaagg catcgaccgg gccaccgccc ccctggacgt
     1201 ggtggcttcc gtcctgggag acagcagccg taaatcatcc cgactttata agagtctgat
     1261 tgaaaccggt ctctgctccg agtgctcggc ttccaactac acactcctgg atccaggctt
     1321 gttcttcgtg caagcgactg tgcagcctgg tcagaacccc cggcaggtgg aggcggtcat
     1381 cctggccgaa ttgaatcgca taaacgagag cggcattacc gaaaccgagc ttctgagagc
     1441 caggcagtca atcgtgaagc gcttcaagct ttccttgagc gatcctatga atctcaccca
     1501 gtctctgact gaaggaatag cggtaggctc ctggcaatgg tggtgcgctt atcctgacag
     1561 tgtggcaaaa gtaagcctgg cagaggcgaa agactttgcc ggccgaactt ttaccgagaa
     1621 caatcgcact gtcggctact atctgcccaa accgacttcc gagagttcca ccgcctccac
     1681 tggttactac cagtttcccg cgcccaagcc ggtcgaattg ccggccctgg ccggtggaga
     1741 gttggaacag acccagggct ctttcccttt tgccgggctt ctaactgcag gcagcggcaa
     1801 gagcgaaatt tccccccgcg tcaagaaagt ggttctggaa aacggtctta ccgtgctgct
     1861 ttatcccatt gcgcgctcgg gtacggtggc tatctcaggc aaaattagag ccggcgatta
     1921 cttcgcccgc gcgccccata ccgagcgcaa cgacctgttg gccaggcttt tgagtttcgg
     1981 caccaagacc tacagcaagc aagagctggc tcaaaaactg gaatctatgg gcgcctccct
     2041 tgatttcgat gccgagacct tcttccataa tttcgaaacg gaagtgacta cccaggacct
     2101 tcccctcatg gttaagcttc tggcttcttg catgatcgaa ccgagcttgc gcaatgaaga
     2161 cctgaaagaa gtaagtcaga tttatgccgc ccaactgaag gaagaatcga ctcaaactgc
     2221 ctctctggcc tggaacaaag tactgggcac gctctataaa aaagactcgg tgtatcacgc
     2281 cgacggtttc gacgagcaaa tcaaagcggt aagcgatacc tccatagaag aaatccgggc
     2341 ccatcac
//
LOCUS       DAOQNZ010000610         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27820_length_2341_cov_3.780402, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000610 DAOQNZ010000000
VERSION     DAOQNZ010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27820_length_2341_cov_3.780402"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKW73_11190"
     CDS             <1..292
                     /locus_tag="PKW73_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73897.1"
                     /translation="SNIELVRAQQLLGRHHDLVRLKEALLKYEKKRERKPARDCKKRE
                     LSKEQLAEQKSKLSECLSRVSLELNRVDDEIKRVKQELPYGLRPYRVSPLIG"
     gene            480..1469
                     /gene="trxB"
                     /locus_tag="PKW73_11195"
     CDS             480..1469
                     /gene="trxB"
                     /locus_tag="PKW73_11195"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNA73898.1"
                     /translation="MVIKKKVTILGSGSAGLTAAIYAARANLEPLVIQGLQAGGQLTI
                     TSDVENFPGFPNGILGPELMEEMHKQAERFGTQFIYDNAESVDLSSRPFKLKTSSDEI
                     HTDCLIIGTGASAKLLGLPSESKLMGHGVSACATCDGFFFRDKEVFVVGGGDTALEEA
                     TFLTRFASSVTVIHRRDSFRASAIMANRAKSNEKVKFILDSVVEDILDVEEGQVTAIR
                     IRNIKTNVVTEHPADGVFIAIGHKPNTELFVGQLELTENGYIKTDGVKTSVKGVFAAG
                     DVQDELYRQAITAAGTGCMAALEAQWLLENEHEEGNSVGKPQAEAKEPVAQKG"
     gene            1481..2215
                     /locus_tag="PKW73_11200"
     CDS             1481..2215
                     /locus_tag="PKW73_11200"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNA73899.1"
                     /translation="MAQGIISRVDSSAWLQAIVVEHPCFKHEMFDRLSSLAKTGSLSL
                     KQVAALLKNYDAHASHLRRLLLKAATIMPEEAVGFILENVRNEYGNGVVEGRHQLQLQ
                     DLAWQSGVAPDDYRLTPVQSGIKTFIKAVTPFYFPGSATIPANLKGKGGGARAAIAAG
                     AITATEIMAMEEFKALQKAFSSLGLQHHIWFDHVTVESEHSQESIALACFFQERGQKS
                     SVEYGLKGVLDANVHLYDGLNACLSV"
BASE COUNT          602 a          643 c          621 g          475 t
ORIGIN      
        1 ctccaatatc gaactggtga gagcccagca acttctgggt cggcatcatg acctggtgcg
       61 cctgaaggaa gctttgctca aatatgaaaa gaagagagag cgaaaaccgg cccgagactg
      121 caaaaaaaga gaactatcta aggagcagct agccgagcag aagagcaaat tatcagagtg
      181 cttgagccgg gtcagcctgg agctgaaccg ggttgatgat gaaatcaaga gagtcaagca
      241 ggagttgccc tacggtttaa gaccctaccg ggtcagcccc ctgatcggct gagaggcaga
      301 ctgaaagtgc ccactaaagg cagagatgcc aagggtactg cttaacttat cgcttaagtt
      361 caaatgaaac tgttcaaact aagctcatca acaatacaat tagaggtgtt ctatagggag
      421 cgctccggat ggccgtaagc tggccgcggt cgcctctttt cctccaggtg gagcttgcca
      481 tggtaataaa gaagaaagta actatcctcg gttcgggttc agccggcctc actgccgcca
      541 tttacgcggc aagagccaat ctcgagcccc tggtcattca aggtctgcag gcaggcggcc
      601 aacttaccat cacctccgac gtggaaaact ttcccggctt tcccaatggc attctcggac
      661 cagagctcat ggaagagatg cataagcaag ctgaacgttt cggcacgcag ttcatttatg
      721 acaacgccga atcggtagat ctctccagcc gccctttcaa actcaaaacc agctccgatg
      781 aaatccacac ggactgcttg atcatcggca ccggggcctc ggcaaaactg ctcggtctcc
      841 cctcggaaag caagctcatg ggtcacggag tatcagcctg cgccacctgc gacggcttct
      901 tcttccgaga caaagaagtc ttcgtagtgg gcggcggcga caccgcgctg gaagaagcca
      961 ctttcctcac tcgttttgcc tcctccgtca cggtaattca cagaagagac tctttcaggg
     1021 cttcggccat catggccaac cgggccaagt ccaatgaaaa agtgaagttc atcctcgaca
     1081 gcgtcgtgga agatattctc gatgtggaag aaggccaggt tactgccatt cgcattcgca
     1141 acataaaaac caatgtggtg accgaacacc ctgccgacgg cgtcttcatc gccatcgggc
     1201 acaaacccaa caccgaacta tttgttggtc aactggagct tacggaaaac ggctatatca
     1261 aaacagacgg cgtgaagacc tccgtgaaag gcgtcttcgc cgccggcgac gtgcaagacg
     1321 aactatacag gcaggcaatc accgccgccg gtaccggttg catggcggcc ctggaagcac
     1381 aatggctttt ggaaaatgaa cacgaagaag gaaacagcgt gggaaagcct caggctgaag
     1441 ccaaggaacc tgtagcccag aagggctgag gttggtgctt atggctcaag gcataatttc
     1501 ccgggtagac tccagtgcct ggctgcaagc aattgtggtc gagcatccat gcttcaagca
     1561 tgaaatgttc gaccgcctca gctccctggc caagaccggc tctctttccc tcaagcaagt
     1621 ggccgccctc ctgaaaaact acgacgccca tgcctctcat ctgcggcggc tgcttctgaa
     1681 agcagctacc ataatgccgg aagaagccgt cggtttcatt cttgaaaatg ttcgcaatga
     1741 atacggcaat ggtgttgtag agggacgcca ccagctacag ttgcaagatc tggcctggca
     1801 aagcggtgtg gcgccggacg actatcggct cacaccggtg caaagcggta tcaaaacctt
     1861 tatcaaagcc gtcacgccct tctactttcc cgggtcggct accattcctg caaatcttaa
     1921 gggtaaaggc ggcggcgcca gagcggccat tgcggcagga gccatcaccg ccacggaaat
     1981 catggccatg gaagaattca aagccttgca gaaggccttt tccagcctcg ggctgcagca
     2041 ccatatatgg ttcgaccacg tgactgtgga atcggaacac agtcaggagt cgatcgcact
     2101 tgcctgcttc tttcaggagc gcggccaaaa atccagtgtc gagtatgggc tcaaaggcgt
     2161 gctggatgcc aatgtacacc tttacgacgg gctaaatgcc tgtcttagcg tttaatcgaa
     2221 ggacagcttg ccaatgaccg ggtcagaaca actaaatctg gccatggtag tgagaatgtt
     2281 cgaccccgcc gggggcgggc tggaacttta tgccttcaag ctggttcaag ccctgctgca
     2341 a
//
LOCUS       DAOQNZ010000611         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27825_length_2341_cov_3.031059, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000611 DAOQNZ010000000
VERSION     DAOQNZ010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27825_length_2341_cov_3.031059"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="PKW73_11205"
     CDS             <1..1736
                     /locus_tag="PKW73_11205"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA73900.1"
                     /translation="AASAKWLKAWREAGAGRSVRCVWSKRAHSNAWQFDSTEEFWRGL
                     GLSDKPRYGFQLFKDGVHCGDALVSDPLKYSVLSNLGPGGGPTARLPKVMRLTSVSPL
                     RTFENGEEPFDERNWAVIDVQGKQIKKAPVSIAYALRSGFPPPHAEDRPFDYRWPVDF
                     AEEEDLEYLDEKIEGEDIESVSPPHEEVLYGLVSKEGKILLEPKYRFIGPFSEGLARV
                     WDGDKKVGYVDRSGTVVIKMQFDAAETFYEGLAAVRKDGKWGFIDKTGNLAIPYSFDS
                     VRRFSEGVAPARRGAKFGYIDKTGRFVIPQQFGRARHFVGGLAYVQIDGQRAYIDHTG
                     KPMGGKMYDVLERFADGRAVFRLKGKYGYIDEKGREVIGPQFTKASAFRNGVAEVMIG
                     STRQSIDCSGKFVKQPPTHSDFWEGPITEGLIRTQEKWKYGFKNRSGKFVIRPSFDQA
                     GVFANGFCPVQVGEKWGVINTAGNLVIQPQFEELHGMFSEHLIAARTGEKWGVIDEKG
                     AVVVKPLYDRIFPFSNGMAIIQDGLKFGYINARGEVVFAPQFDAAHQFDDKGIAHVSK
                     RVHELEVDELP"
BASE COUNT          553 a          506 c          694 g          588 t
ORIGIN      
        1 atgcggcttc tgcgaaatgg ctgaaagcct ggcgggaagc cggtgctggc cgatctgtcc
       61 gttgcgtctg gagcaaaaga gcacactcta acgcatggca gttcgatagt acagaggaat
      121 tctggcgggg tcttggacta tcagacaaac cgcgatacgg ttttcaactt ttcaaagatg
      181 gcgttcactg tggcgatgcc ctggtcagtg acccgcttaa gtattcggtg ctttccaatc
      241 ttggtcccgg gggagggcct acggcgaggc ttcctaaagt tatgcgatta acttccgttt
      301 cgcccctgcg cacctttgag aatggagagg agccttttga tgagcgaaat tgggccgtta
      361 tagatgtgca gggcaagcaa attaagaagg cgccagtatc tatcgcctat gctttacgct
      421 ctggctttcc acctccgcat gcagaggata ggccatttga ttacagatgg ccggtagact
      481 ttgctgaaga ggaagacttg gagtatttgg acgaaaaaat agagggcgaa gatattgaat
      541 cagtttctcc cccgcacgag gaggtgctct atggtttggt cagcaaagag ggcaagattc
      601 tcttagagcc caagtatcgc tttattggac cattttcgga aggactggcg cgggtgtggg
      661 atggtgataa aaaggtcggc tatgtcgaca gaagcggaac ggttgtgatc aagatgcagt
      721 ttgatgcagc cgagacgttt tatgagggtc ttgccgccgt tcgcaaggat ggtaagtggg
      781 gattcattga taagaccgga aaccttgcca ttccgtattc ctttgattca gtcaggcggt
      841 tttcggaggg agtagcgccg gcgcgcagag gtgctaaatt cggctacatt gacaagaccg
      901 gaagattcgt gatccctcag cagttcggcc gagccaggca ctttgtcggt ggtctggcat
      961 acgtgcagat agacgggcaa agggcttaca tcgatcatac aggaaagcca atggggggca
     1021 aaatgtatga cgttcttgag cggttcgctg acggtcgggc ggtgtttcgc ctcaaaggca
     1081 aatatggcta tattgatgag aagggtcggg aggttatagg cccacagttc actaaagcca
     1141 gcgcatttcg aaacggagtc gccgaagtta tgattggctc cacaaggcag tcgattgatt
     1201 gctctggcaa gttcgtcaag cagccgccga cacacagtga cttttgggaa ggtccgatta
     1261 cggagggtct gattaggact caagagaagt ggaaatacgg gtttaaaaac cggtctggaa
     1321 aatttgtgat tagaccgtcc tttgatcagg cgggagtttt cgcaaatggg ttttgccctg
     1381 tgcaagtggg tgagaagtgg ggcgttatta acacggcggg aaatcttgtt atccaaccgc
     1441 agttcgaaga gcttcacgga atgttttcag agcatttgat tgccgcccgc acgggagaga
     1501 agtggggtgt tatcgatgag aagggtgccg ttgtggtaaa accgctttac gatcgcatct
     1561 tcccgttctc gaatggaatg gccataattc aagatggact caaattcgga tacatcaatg
     1621 ccagaggtga agtggttttc gctccacagt tcgatgctgc tcatcagttc gacgataagg
     1681 gcattgctca tgtcagtaag cgagtccacg agctggaagt agatgagctt ccttaagttt
     1741 tactagattg agtgaattga cctttgcgct cgcaccgctg aaaggtttca gaacatggcg
     1801 gtggtgtcac gagaagctgg ggcgattcct catcaatgcc tttctatgtt caatccggtc
     1861 gtgaccaacc tgcgggcgtt acataccttt cgataccggg tcataaattt ctggatgaag
     1921 gcgcttcgct tcgcaactgg tacgggctct gaaactttgt tgaaggagct gttccgctcg
     1981 cgctgggagt cctgggactg ccgagagata cgctagggaa gatcatgctc aatattgtcg
     2041 gagacaggct gagccatgct aaacttgccg ggttaggtgc tgtcgataaa acctgctatg
     2101 tgcttggtgt ccaccgtggc gccatatcgt tggaactgct caagaagcgc ttgatggtcc
     2161 tttatacggt gatgctctta agcatggacg gggactctgc ctaccgctat ggatgcgagg
     2221 aaatggcagc gccaacagta gttttgttat ttagtcctgg cagatccggg tagcatgcca
     2281 gattatttga agccctgttt atctagcacc ggctatgcgc tgaaaagcaa ggccccgaga
     2341 g
//
LOCUS       DAOQNZ010000612         2337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27899_length_2337_cov_4.141104, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000612 DAOQNZ010000000
VERSION     DAOQNZ010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27899_length_2337_cov_4.141104"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..599)
                     /gene="queF"
                     /locus_tag="PKW73_11210"
     CDS             complement(111..599)
                     /gene="queF"
                     /locus_tag="PKW73_11210"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNA73901.1"
                     /translation="MSKKSQFKNLGKATAKEKQNYDNPDPAILEVFDSPFADKKANRH
                     SVIGTIHIEAPEFTTVCPITGQPDFATIVIDYRPDQLCVESKSLKLYLGAFRQFPEFH
                     EACVNRIANDLIDKLKPLSLTVEGRFTPRGGIPFWPVARYESSGKPLPSGKAKKTRPA
                     KG"
     gene            743..1498
                     /locus_tag="PKW73_11215"
     CDS             743..1498
                     /locus_tag="PKW73_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNA73902.1"
                     /translation="MFRSFSVSVLTILSLWLTISPAHSLPRKTYFKSSGKSIAVDWFY
                     PEKLANLPRVDARQSTPIVIILHGAGGLGTDSRDGFFQQLAQELTRQGNSCAIVHYMD
                     QTNMQAADAVALAKHFGTWLKTVRAATSYILELPLADKNNVNILGHSLGAQLALHTAD
                     GNPKLASVVDMAGSFVLPTGAIKAMPPILVLHGTKDTTVPLSRERQLISVLKRTNSTY
                     EEHILSGVDHSFSGVSFDTLTKIINAFLIKHRR"
     gene            1558..>2337
                     /locus_tag="PKW73_11220"
     CDS             1558..>2337
                     /locus_tag="PKW73_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73903.1"
                     /translation="MRHNTSTRKHQSAVKPSQKRAGFLLLGSIIWLSSTSNCAGVGAE
                     TISSAQTGKHYENTANWRKAIESYNQELALSPRDAHCLARRGYAHARLGNFKQSFVDF
                     SAAIEADSACPDAYSRRARVYTGLGFIQLAKQDAQKALTLIGDAPSEPNLLLEHAGLL
                     YIVENKQVADLESKKVLDLCKTNKDSSTLLNLAGAHFNLGDFKDTVYYTTQALCLEPT
                     NSYLHLIRGAACYQLRQYSMAVTDLNCHINNELIAPMALCAR"
BASE COUNT          651 a          619 c          526 g          541 t
ORIGIN      
        1 aaccagggcg ctcatttatg ccagtcctta agcaactgct tgaaaatcta ccgaaagatt
       61 ttaccagagg ctaaggcagt ctacggacgg gcgtcttaag ctcttaacaa ttaacccttg
      121 gcagggcggg tcttcttcgc tttaccggac ggcaacggct tgccgctgct ctcgtagcga
      181 gccaccggcc agaaaggaat accgcccctg ggtgtaaagc gcccttccac cgtcaatgag
      241 aggggtttca gcttgtcgat caggtcattg gcaatgcgat tgacgcaagc ctcatgaaac
      301 tctggaaact gacgaaatgc ccccaggtac agcttcagcg atttgctctc gacacaaagt
      361 tggtcaggtc tgtagtcaat aacaatggtc gcaaaatcag gctgcccggt gatggggcag
      421 accgtcgtaa actcgggagc ttcgatatga atagtgccga ttacactgtg acggttcgcc
      481 ttcttatcag cgaagggact atcgaaaacc tcaagaatag ccgggtctgg attatcgtaa
      541 ttttgcttct ctttagcagt ggctttgccg agattcttaa actgactttt ctttgacatt
      601 ggtagttgac cgttttctcc agggtctgtt aattcaacca aaaaaccaac gcccccggca
      661 tgctaatgag agtttcctca tgcttttata tagggatgtg cttcggcgat ctagattctc
      721 ggcaattctt gaggcaaaat ccttgttcag aagcttctca gttagcgtat taacaattct
      781 cagcctatgg ctaaccataa gcccagccca ttcactgccg cgaaaaacct atttcaagtc
      841 atccggcaaa agcatagctg tagactggtt ctacccagag aaattagcaa accttcctcg
      901 ggttgatgca agacaatcca cacctattgt aatcattctg cacggcgccg gcggcttagg
      961 aacagacagc cgcgatggct tcttccaaca attagctcag gaactaacca ggcagggtaa
     1021 cagctgcgcc attgtgcact atatggacca gaccaatatg caggcagcag atgccgtcgc
     1081 cctagcaaag cactttggca cctggctgaa aacagtcagg gccgccacat cctacatact
     1141 ggagcttccc cttgccgaca agaataatgt caacatactg ggacatagcc tgggagctca
     1201 attggctctc catacagcag atggtaatcc aaagcttgca tcagtagtgg atatggcagg
     1261 cagctttgtc ttaccaaccg gggcgataaa agccatgcca ccaatattgg tattacatgg
     1321 aacaaaagac accacggtgc cactaagcag agagcgccaa cttatcagtg tcctcaaacg
     1381 cacaaacagt acctatgaag aacatatact gagtggagtc gatcattctt tctcaggtgt
     1441 gagctttgac accctcacaa aaatcattaa tgcgtttctg atcaaacaca ggcggtagag
     1501 tattaccatt tacattaggg tgagcgaact tttcggcaca agaggcataa gacagccatg
     1561 aggcataaca cctctaccag gaagcaccaa tcagctgtca aacccagcca aaagagagca
     1621 gggtttcttc ttctgggctc tatcatctgg ctctccagca catccaattg cgctggcgtt
     1681 ggtgccgaaa cgatctcatc tgcacaaact ggcaaacact atgaaaatac cgcaaattgg
     1741 cgaaaggcca tcgagagtta taatcaagag ctggctttat cacccagaga cgcccattgc
     1801 ctggcaagaa gaggctatgc acacgctcgc ctgggaaatt tcaagcagtc ctttgtcgac
     1861 ttctctgcgg ccattgaagc tgattcggct tgcccggatg cctattcaag acgagcgcgg
     1921 gtctacaccg ggttagggtt cattcaactt gccaaacaag acgcgcaaaa ggctctcacg
     1981 ctaattggcg acgccccctc agagcccaac ctgctgcttg agcacgccgg tttgttgtat
     2041 atcgtcgaaa acaagcaagt tgccgaccta gaaagcaaaa aggttctaga cctctgcaaa
     2101 acaaacaaag actcaagcac actcctaaat ctggccggag cacatttcaa tttaggagat
     2161 ttcaaagata ccgtctatta cacaacacaa gccctatgcc tggagccaac aaactcctat
     2221 ctgcacctca ttagaggagc cgcctgttac caacttagac aatattcaat ggcagtaacc
     2281 gacctaaatt gccatataaa caatgagctc atagctccca tggccctctg cgccagg
//
LOCUS       DAOQNZ010000613         2337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27900_length_2337_cov_3.911919, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000613 DAOQNZ010000000
VERSION     DAOQNZ010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27900_length_2337_cov_3.911919"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..848
                     /locus_tag="PKW73_11225"
     CDS             285..848
                     /locus_tag="PKW73_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNA73904.1"
                     /translation="MVDDYRESPQEKPKFANETRSNKREIDLFPALQDRRMMALGSAR
                     QVTQAPTRLGTQISIFTPVSFEEALEIVECLRARAATTISLENMKKLDASRLVDFVAG
                     ASAALDGDFHKLSEQVYVFCPANIKITAQSKVAAAAAAASTTYSEAFAGSSESISNLN
                     NLGMGALDFLYPGRNQDSGNASTWPTS"
     gene            complement(1073..2122)
                     /gene="mnmH"
                     /locus_tag="PKW73_11230"
     CDS             complement(1073..2122)
                     /gene="mnmH"
                     /locus_tag="PKW73_11230"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNA73905.1"
                     /translation="MTWREMSVEELIALKKVALIDVRSPCEYSVENIPGAVNVPLFTD
                     HEREMIGTIYATEGELVARRRALRILGPKISDLVEEIVQHRQSGAPLVIHCWRGGLRS
                     ESVASCLSIIGVDSWRLTGGYKAWRRHVVADFEAEVFQFEALVLDGLTGSGKTELLQA
                     LEDAGEPVLDLEALAGHRGSVFGALGLGAQPTQKNFEAKLWNKLRSFKSKYLFIEAES
                     RKVGNLRVPDFLYKRIQEGKRILVEGSLTVRARRILGEYASGGDSGPDSQVLSDAMAA
                     LALIKDRISGEKVKVIMEMVERGDFQDAVQILLTDYYDPLYRRNMDRPALLTVNSDKR
                     EECLKQLLELAGELN"
     gene            complement(2126..>2337)
                     /locus_tag="PKW73_11235"
     CDS             complement(2126..>2337)
                     /locus_tag="PKW73_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73906.1"
                     /translation="VEQILKEGERSKLLSADGVKQIIEDGLTAYFAGTMKIRPLLQVV
                     AMRANAMLREAQPDPDRITFKQGKK"
BASE COUNT          566 a          686 c          534 g          551 t
ORIGIN      
        1 tgatgcggcc atacttgagg ccttcggaca taaggtagaa gtgtttgaag gatttactca
       61 caacattaag gttactgtag ccgatgattt taagacttgc caacttttag cccccttata
      121 tctctccaga agccccatgg aaccatagct ggtgcatatt gaacaacctc taaatttaca
      181 tgccgcacca cttacggcac cacttgcaag ttctagataa atgactagaa ttgttccatc
      241 tccattttta tccgtattta tacaaaacac aaaaagggac agccatggtt gacgactata
      301 gagaaagccc tcaggagaag cctaaatttg ccaatgagac ccggtccaac aagcgcgaga
      361 tagatctctt tcccgccttg caagatcgtc gaatgatggc tcttggttca gcccggcagg
      421 ttacccaagc tccgacaaga ttaggcaccc aaatctccat ttttaccccg gtctccttcg
      481 aagaggccct ggaaattgtt gagtgtctgc gagcaagagc agccaccacg atttccctcg
      541 aaaacatgaa gaagctagac gccagccgtc tggttgactt cgtagccggt gcttctgctg
      601 cgctcgacgg tgattttcac aagctcagcg aacaagtgta tgtcttctgt cctgccaata
      661 ttaaaatcac tgcccagagc aaggttgctg ccgctgctgc tgccgccagc accacatata
      721 gtgaagcctt tgccggttcc agtgagtcaa ttagcaatct aaacaacctg ggaatgggcg
      781 cccttgactt cctttacccg ggacgaaatc aggattcagg aaacgcctca acctggccta
      841 cctcctgaca agaaaagtag tcagggtgaa ctgaacttgg gttaatcaaa tttaaggtgt
      901 gccagtcggc gcctttcaac gcggccttca agttttttaa cagccacccc tgatcctcgc
      961 tggtatcatt gagatacagc taatacatcc tttagctgtg tttcctcctt acctgatcca
     1021 ccacaacatg tctgcccctc cggtttcgga ggggcagttc tcttaggcgg gttcagttaa
     1081 gctccccggc cagctcaaga agttgcttca ggcactcttc ccgtttatcg ctattgaccg
     1141 taagcagagc cgggcggtcc atattacgcc tgtaaagagg atcgtaataa tccgtcagca
     1201 aaatctgcac agcatcctga aagtcgcctc tttccaccat ctccataatc acttttactt
     1261 tctcgccgct gatacggtcc ttaatcaagg caagagcggc catggcatcg gagagcacct
     1321 gggagtccgg tccggaatca ccgccggagg catactcgcc caggatgcgc ctggcccgca
     1381 ctgtcagcga gccttccacc agaattcgct tgccctcctg aatgcgcttg tataggaagt
     1441 caggcacccg taggttgcca acttttctgg actccgcctc aatgaagaga tacttgctct
     1501 taaaactacg cagtttgttc cagagcttcg cttcgaaatt tttctgagtg ggctgcgctc
     1561 ccagaccaag ggcaccaaaa acagaccccc gatggccggc cagagcttcc aaatcgagca
     1621 ccggctcacc ggcatcctca agggcttgca aaagctcggt cttgcctgag ccagtcagcc
     1681 catccagtac cagagcttca aactggaaaa cttccgcttc aaaatcggcc accacgtgcc
     1741 gccgccaggc cttataaccg cccgtgaggc gccagctatc cacaccaata atgctcaggc
     1801 aactggccac cgactcagat cgcaaaccgc cgcgccagca gtgaataacc agaggcgcgc
     1861 ccgactgcct gtgctgcaca atttcttcca ccagatcgga gatcttcgga ccaaggatgc
     1921 gcaaggcccg acgcctggcc accagttcgc cctcggtggc gtatatggtg ccgatcatct
     1981 ctctctcgtg atcggtaaag agcggaacat taacagcacc tggaatattc tcaaccgagt
     2041 attcgcaggg agaacgcaca tctattagtg ccaccttctt gagagcaatg agctcttcta
     2101 cgctcatttc ccgccaggtc atggtctact tcttgccctg tttgaaagta atacggtccg
     2161 ggtcaggctg tgcttcacgc agcatggcat tggccctcat ggccaccact tgcagaagcg
     2221 gccgaatctt catggtaccg gcgaaatatg cggtcagacc gtcttcaata atctgcttga
     2281 caccatcggc gctcaagagt ttggatcgct cgccttcctt gagaatctgt tccaccc
//
LOCUS       DAOQNZ010000614         2337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27903_length_2337_cov_3.505697, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000614 DAOQNZ010000000
VERSION     DAOQNZ010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27903_length_2337_cov_3.505697"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PKW73_11240"
     CDS             complement(<1..1299)
                     /locus_tag="PKW73_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73907.1"
                     /translation="MNEALKAKLVSKLKSTFCVYCASIFLSFPIAVTLIAMITPALVP
                     LQAETAAYGVKNFINRNGSHDLNSLEITRLLERYNLSWLYITDGSSKPSSETSRFAPN
                     MVDYPMRSGTINLRGTEYYEAVLALPTGFMIHVGMDCTYPGLPLSALEDILFFWNMPF
                     RGGTVIAIFLLAGGINFIVLSRIFLTPLAKFRQDIDNLAATAAPSLVAFDKITLSPMA
                     SSELWNLKKSLHSLFESLGHHEKGKERPSAAPVKDGPWQNPSANELQAVSDGRLPGVG
                     ERKAKTTVHALKALKQSEETFGENITEGVLERFPGIVSGVVFMKGDVLDGKSTSDSIV
                     KSAGLNTGQLVELQNLNLLPIFEQARTSGKAINLGPMSLKRTGLDAIMHKLNAQHIVL
                     APIRHRRRYLGFMLVLTTAALGPDQIRALERLLDQTASVYN"
     gene            complement(1303..>2337)
                     /locus_tag="PKW73_11245"
     CDS             complement(1303..>2337)
                     /locus_tag="PKW73_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73908.1"
                     /translation="SGCDLSVTARFGSLLVGLPLLLCSIYMGRKLFPDRLSQLALPLM
                     VVFHPQLILVHTYINNDSTAITLAAVVLVLLTRMLFLAPSLPLTFITGLTCGFLSLTK
                     YSAYAVFPATLLGLLLAFHKAKTPPLKILLHLFVCAASALAVSLWWFLRTFKLYPGDL
                     LGTKTMFRTWAVTYHRDLNFYMTPWQVMKEHRWWRSILFSFWGLFGYMDIYLWRWVYW
                     TYFGYMLLALATSLKAALVCLKNWKKEQPSGQAQARAYTWAVLGATLVGNIMAMVYAS
                     TVNLGGGQGRYLFPSEIPIMSLLVAGLHLTGNWRKPLLLSLVIFNALVSIGCFIYLFP
                     RYGFHFIRTY"
BASE COUNT          564 a          537 c          728 g          508 t
ORIGIN      
        1 gttgtatacc gaggctgtct ggtccagcag gcgctccagg gctcttatct gatccggtcc
       61 cagggcggca gtggtgagca ccagcataaa gcccaggtag cgcctgcggt ggcgaatcgg
      121 ggctagcacg atgtgctggg cattgagctt gtgcatgatg gcatcgagtc cggtgcgctt
      181 caaagacatg ggacccagat ttatggcttt gccggaagtg cgtgcctgtt cgaatattgg
      241 cagcagattg aggttttgca gttccaccag ttgaccggtg ttgagccctg ccgatttgac
      301 gatactgtcg ctggtgctct tgccgtccag gacgtcgcct ttcatgaaga caacaccgct
      361 cactataccg gggaatcttt ccagcactcc ttcggtaata ttttctccga aagtttcttc
      421 cgactgtttc aaggctttga gagcatgtac ggtggttttt gctttacgtt cacctacccc
      481 gggcaggcga ccgtctgaaa cggcttgcag ttcgttggcg gagggatttt gccagggtcc
      541 atctttgacg ggagcggcac tgggtcgctc tttccctttt tcatggtgac ccaggctttc
      601 gaacaaactg tgcagcgatt ttttcaaatt ccacaattcg cttgatgcca taggggaaag
      661 agtgattttg tcgaatgcca ccagacttgg tgcggcggtg gcggccagat tgtctatatc
      721 ctggcggaat tttgccagag gagttagaaa gatgcggctc aaaacaatga aattgatacc
      781 gccggccagg aggaaaatgg ctatcaccgt gccgccccgg aaaggcatat tccagaagaa
      841 gaggatatcc tccagggcgg aaagcggtaa gccggggtag gtgcaatcca tgcccacatg
      901 gatcatgaag cccgtgggca gggccaggac tgcttcgtag tattcggtgc cgcgtaagtt
      961 gatggtgccg ctgcgcatgg ggtagtccac catgttcgga gcaaagcgcg aagtctccga
     1021 cgaaggtttg gaactgccgt cggttatata gagccaggaa aggttgtatc tctccaaaag
     1081 gcgtgtgatt tcgagagaat tgagatcatg gctgccgttg cgattgatga agtttttgac
     1141 gccataggcg gcggtttctg cttgtaaggg caccagagcc ggcgtgatca tggcaatcag
     1201 ggtgactgca atggggaaag agaggaagat ggaagcgcag taaacgcaga aagttgattt
     1261 cagcttgctg actagcttgg ctttcaatgc ttcattcata ggtcagtatg tcctgatgaa
     1321 gtgaaaacca tagcggggaa acaggtagat gaagcagcct atggaaacaa gtgcgttgaa
     1381 tataaccagg ctgagcaaaa ggggtttcct ccagttaccg gtcaaatgaa gtccggctac
     1441 aagtaaggac ataataggga tttccgacgg aaaaagatag cgcccctgtc caccgcccag
     1501 gttcactgtg gaggcataga ccatagccat gatattgcct accagtgtgg cgcccagcac
     1561 ggcccaggta taggctctgg cttgagcctg accggaaggt tgctctttct tccaattttt
     1621 caggcagacc agagccgctt tcagggaagt ggcaagagcc agaagcatat aaccgaaata
     1681 tgtccagtag acccagcgcc acaggtagat gtccatgtag ccgaagagtc cccagaacga
     1741 gaagaggatg gagcgccacc agcggtgctc tttcatgacc tgccagggcg tcatgtagaa
     1801 attgagatcg cggtggtagg tgacggccca ggtgcggaac atggtcttgg taccgagcag
     1861 gtcgccagga tagagtttga aggtgcgcaa aaaccaccag agcgaaaccg ccagggctga
     1921 ggcggcgcag acaaagagat gcaagaggat ttttagaggc ggtgttttgg ctttgtggaa
     1981 agccagaagt agccccagaa gtgtggccgg gaaaacggcg taggctgagt atttggtgag
     2041 ggagagaaag ccgcaggtga gaccggttat gaaggtaagg ggcagactgg gggcaagaaa
     2101 gagcatcctg gtcagaagca cgagcaccac tgccgccagg gttatggctg tggagtcgtt
     2161 attgatatag gtgtgcacca ggatcagttg ggggtgaaac accaccatga gcggcagggc
     2221 tagttggctg aggcggtccg ggaagagctt gcgtcccata tagattgagc acaggagcag
     2281 gggcagcccc acaaggaggc taccgaatct agccgtcacc gaaaggtcgc agccgga
//
LOCUS       DAOQNZ010000615         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27938_length_2335_cov_3.697807, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000615 DAOQNZ010000000
VERSION     DAOQNZ010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27938_length_2335_cov_3.697807"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="PKW73_11250"
     CDS             <1..1938
                     /locus_tag="PKW73_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNA73909.1"
                     /translation="QIRDIYFPRVGQDNQTVGRVNHLGFWVDGQFSWISEDWHIEKKY
                     LKETLVTDVLLRNDRLGIEARFHDLVDAFDNVLLRELRISSIDGKEHQVRVFFHQDFS
                     ISENEVGDTAYYDPRTSSVIHYKKNRYFLINTKTQLKTGISDWSVGKKGVDGLEGTYK
                     DAEDGKLGGNPIVQGSVDSTVGLHLNVPRTGEVVGHYWMCLGRSYEEVAKLNAVMAAG
                     EQAEQMVPRNQNYWRLWVNPEHIDFHGLPAKVVELFKRSLLTIRTQVDNGGAIIAAND
                     HDITQFARDTYSYMWPRDGALVAYAMTKAGHRDLTQRFFRFCNEVMKEEGYLMHKYNP
                     DKSVASSWHPWLKDGKEVLPIQEDETALVIWSFWHHFKKFRNIEFVRTVMDKIIFDGA
                     DFLCRYRDETTKLPLPSYDLWEERWGVHLFTVSSVIAGLTAAANFCQAVGELTRAHHY
                     QEVAEEVKEAMCEYMWSKEHKRFCRMATRTESGYDLDMTIDAAMYSIFAFGEMNPHDS
                     KVVSTMNAIRERLWIKTEVGGLARYENDYYHQISQDLENVPGNPWFICTMWLAQYDIA
                     AARTTDDLKSAVVLMEWVANRALSSGVLAEQVHPYSNEPLSVSPLTWSHATFVTCVLE
                     YLDRKKQLMTENVFSHTIVPV"
     gene            complement(1954..>2335)
                     /locus_tag="PKW73_11255"
     CDS             complement(1954..>2335)
                     /locus_tag="PKW73_11255"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73910.1"
                     /translation="LTGKLSKAKELYQKALELREKSLSPDNPAIADSLLILGTLEFKS
                     RNLDKAESLFNRAMDINTRNGSAGKDGLSDAQFCLAMVCDAKKDKARALSLAKSSYEL
                     RQQSFGEEDQRTVEAKSLMQRLSK"
BASE COUNT          562 a          628 c          623 g          522 t
ORIGIN      
        1 caaatcagag acatctactt tcccagggtg gggcaggaca atcagaccgt aggacgggtc
       61 aaccatctgg gcttctgggt ggacgggcag ttttcctgga ttagcgaaga ctggcatatt
      121 gaaaagaaat atctaaaaga gaccctggtc accgatgtgc ttttgcgcaa cgacaggctc
      181 ggcatcgaag cgcgcttcca cgatctggta gatgccttcg acaatgtgct tttgcgagaa
      241 ctgagaatct catcgattga cggaaaagag caccaggttc gcgttttctt ccatcaggat
      301 ttcagtattt cggaaaatga ggtaggggat acagcctatt acgacccgcg tacctcatcg
      361 gtgattcact acaagaaaaa ccgctacttc ctgatcaata cgaaaaccca attgaaaacc
      421 ggcatcagcg actggtccgt gggcaagaaa ggggtggacg gactggaggg cacctacaaa
      481 gacgccgagg acggtaagtt gggcggcaat cccattgtgc agggttcggt ggattccacc
      541 gtcggtctgc acctgaacgt gcctagaacc ggtgaagtcg ttggccacta ctggatgtgc
      601 ctggggcgca gctacgaaga agtagccaaa ttgaatgccg tgatggctgc cggcgagcag
      661 gctgagcaga tggtgccgcg caatcaaaat tactggcgcc tctgggtcaa tcccgagcat
      721 atcgactttc acggactacc ggcgaaagtc gtagaactct ttaaacgttc tcttttgacg
      781 attcgtaccc aggtggataa cggcggcgcc attatcgccg ccaatgacca cgacatcacg
      841 caatttgccc gggacaccta ttcttatatg tggccccgtg acggcgccct ggtggcttac
      901 gccatgacca aggccggaca tcgagacctc actcagcgct tcttccgctt ctgcaatgaa
      961 gtaatgaaag aagaaggcta tctgatgcac aaatataatc ccgacaaatc cgtggcatca
     1021 agctggcatc cctggcttaa ggacggcaag gaagtactgc ccatccagga agacgaaacg
     1081 gcgctggtga tctggtcttt ctggcaccac ttcaaaaaat tccgcaacat cgaattcgtg
     1141 cgcaccgtca tggataagat aatttttgac ggcgccgatt tcctctgtcg ttaccgggat
     1201 gaaacaacca agctgccctt gccttcctat gacctctggg aagagcgctg gggagtccat
     1261 ctattcaccg tatctagtgt catcgccggg ctcacggccg ctgcaaattt ctgccaggcc
     1321 gtgggcgaat tgacccgggc ccatcactat caggaagtgg ctgaagaagt gaaggaagcc
     1381 atgtgcgaat acatgtggag taaagagcat aagcgcttct gtcgcatggc cacgcgcacc
     1441 gagagcggtt acgatctgga tatgaccatc gatgccgcta tgtacagtat tttcgccttc
     1501 ggcgaaatga atccccatga ttcaaaagta gtttccacaa tgaatgccat ccgagaaagg
     1561 ctctggatta agactgaagt aggcggtctg gcccgttatg aaaatgacta ctaccaccaa
     1621 atttcccagg atctggaaaa cgtgcccggc aatccctggt tcatttgcac catgtggctg
     1681 gcccagtacg atatcgccgc cgcccgcacc accgacgatc tcaaaagcgc cgtggtactg
     1741 atggaatggg tagcgaatcg cgccctcagc tccggcgtgc tggcggaaca ggtacatccc
     1801 tacagcaacg aacccctctc ggtttcgccg ctcacctgga gccacgctac tttcgtcacc
     1861 tgcgtgctgg aatatctgga tcgcaagaaa caactgatga ccgagaatgt gttttcacac
     1921 accatcgtgc cggtctgagc cggtcggcaa attctacttg gagaggcgct gcatgagcga
     1981 cttggcttcc acggtgcgct gatcttcttc gccgaacgac tgctgcctga gctcataaga
     2041 actcttggcc agacttaagg cccgagcctt gtctttcttg gcgtcgcaaa ccatggccag
     2101 gcagaactgc gcgtcggata gcccgtcttt accggcgctg ccgttgcggg tattgatgtc
     2161 catggcccgg ttgaagagcg actcagcttt atcgagattg cgcgacttga actccagggt
     2221 gcccagaatc aagagagaat ctgcaatggc cggattatcc gggctgagac tcttttccct
     2281 cagttctaaa gctttttgat acagctcctt ggccttgctg agtttgcccg taagt
//
LOCUS       DAOQNZ010000616         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_27963_length_2334_cov_3.356297, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000616 DAOQNZ010000000
VERSION     DAOQNZ010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27963_length_2334_cov_3.356297"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PKW73_11260"
     CDS             complement(<1..926)
                     /locus_tag="PKW73_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73911.1"
                     /translation="MAPKISFKSIQAGLSLALLTFCQGASVQAQAEYKPLSQLAPALA
                     VAGQSQYPPGLSEGAGIWVNLWNYPSRDFEGYAEKLYASGIRNLFIQTSRSNTPAIRQ
                     PEELGQIIEACHKYKIRVIAWSFAELHNPKADAEKLIVAARFRSKNGERLDAIAPNLE
                     KNLHKEAVEAYSKILREALGAYPMVAVVYSPLNRAPAVAITPWKTLAQYYDVIAPMAY
                     WNGRYQTIDAYTYTKRTVERIRELTARPDVEVHVIGDGMGTRAPEIKEFLRGCADSGA
                     QSASLYPDQITTAEQYGAMSLYNDYMPVDARE"
     gene            1295..1855
                     /locus_tag="PKW73_11265"
     CDS             1295..1855
                     /locus_tag="PKW73_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73912.1"
                     /translation="MNKKEEEIQRKLLELETTVLKENTDLSLVEGRTDLTVSAKQAGK
                     SSTSKDEALTKSDGAYFGGLGLIILGLIMVFQHINVGSGLLGMLGLGSGGFLVLFLPL
                     MIGIGMMLFNSKNKWGWLITAGSCVFLIFSVLASLTMTFKAMSLLQMIVLFLPFAIGG
                     SLLVKGMGGPKGVVQAIKSNMPEKSE"
     gene            1882..>2334
                     /locus_tag="PKW73_11270"
     CDS             1882..>2334
                     /locus_tag="PKW73_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNA73913.1"
                     /translation="MTRVFVGSFLEGEEREILARFLAEENKKLKPDQASVIRSVKPEK
                     LHITWQFLGELDENELPKLKETLLDLTGPLFQAANFPFSITYDYAEAWPSTQARVIVA
                     RPRVESKTLTTVARLLREALAPHSRADAAVERNLVFKPHVTLYRLRQAR"
BASE COUNT          595 a          594 c          621 g          524 t
ORIGIN      
        1 ctctcgcggg catcaaccgg catgtaatcg ttgtagagag acatggcgcc gtactgctcg
       61 gcagtggtga tctgatcggg atagagactg gcgctctgtg caccggagtc ggcgcaaccg
      121 cgcagaaatt ctttgatttc aggcgccctt gtgcccatgc cgtcaccaat tacgtgcact
      181 tccacgtcgg ggcgggcggt gagctcacga atacgttcca cagtgcgctt ggtataggta
      241 taagcgtcaa tggtctggta gcgaccgttc cagtaagcca tgggcgcaat cacgtcgtaa
      301 tactgggcca gggttttcca gggggtgatg gctacagccg gagcccgatt caatggactg
      361 tagacaacgg ccaccatggg gtaagcaccc agagcctctc tcaaaatctt ggaatatgct
      421 tctacagctt ctttgtgcag gttcttctcc aggttgggag caatggcatc aagccgctct
      481 ccgtttttgg aacgaaagcg cgccgctacg attagctttt cggcatcagc cttgggattg
      541 tgtagttcgg caaaggacca ggcaattacc ctaattttgt acttgtggca ggcttcgatt
      601 atttgtccca actcctccgg ttgcctgatg gcaggagtgt tgctacggga tgtctggatg
      661 aatagattgc gaataccgga tgcgtagagc ttctcggcat agccttcaaa atcccgactg
      721 ggatagttcc agagattaac ccagatgccg gcaccctcgg aaagacccgg tggatactgg
      781 ctttgaccag ccacagccag agccggtgcc aactgactca agggcttata ttcagcctgg
      841 gcctgcacgg aagccccctg gcaaaaggtc aacagggcca gagaaagacc agcctggata
      901 gatttgaaag aaatttttgg tgccatattt agtaagtcca agctggaaga aaagaaaaat
      961 cgaagtaaag ccaagtttaa gacattataa atgtgatacc agacttatgc cactcaaact
     1021 ttccttttaa accaaactct gccattgaaa attggctccc caagccaaat cgacgcagtc
     1081 gctccgtaat tgaccgactt gcgcccgaaa atttatcggt ctgagccctc cggccgagct
     1141 gtcaatatag ttagagtgag gaacgtccct gggatttagt aaccgaagag ggtatataag
     1201 gagcggaatc accatcgcaa aagagctgca aaaattcggc agcaaaaatt tggctgagaa
     1261 gacgggttaa gtaccaggaa aaggcttgga actaatgaac aaaaaggaag aagagattca
     1321 acgaaaactc cttgagcttg agaccactgt gttgaaagag aatacagact tgagcctggt
     1381 ggagggtcgc accgatctca cggtgagcgc aaagcaagca ggcaagagct ctacctccaa
     1441 agacgaggcc ctgactaaat cggacggcgc ctatttcggc ggtctcggtc taataatcct
     1501 cggtctgatc atggtcttcc agcacatcaa tgtgggctca ggtttactgg gcatgctcgg
     1561 tcttgggagc ggcggctttc tggttctatt tttgccgctt atgataggca tcggcatgat
     1621 gctcttcaac tccaaaaaca aatggggttg gctcatcacc gcaggcagtt gcgtctttct
     1681 catcttctcc gtgctggcca gtctgaccat gaccttcaag gccatgagcc ttttgcagat
     1741 gatcgtgctc ttcctgccct ttgccattgg cggctctttg ctggtgaaag gcatgggcgg
     1801 tcccaaggga gtagtgcaag ccattaagag caatatgccg gaaaaatcag agtagaaatc
     1861 cgaatcaatt agaggaagag attgacccga gtttttgtgg gaagctttct tgagggcgag
     1921 gaaagagaaa ttctcgcccg ctttcttgcg gaagaaaata aaaagctcaa gccggaccag
     1981 gcgtctgtaa ttagaagcgt gaagccggag aaactgcaca ttacctggca gttcctgggc
     2041 gaactggatg agaacgaatt gcccaaactg aaagagacct tgctggacct cacgggaccc
     2101 ctcttccagg cagcaaactt ccccttttcc attacttacg actacgccga ggcctggccg
     2161 tccacacaag ccagggtgat cgtggccaga ccacgggtgg aaagcaagac tctaaccact
     2221 gttgcaagac tcctgagaga agccctcgcc ccccacagca gagccgacgc cgccgtcgaa
     2281 cggaatctcg tcttcaagcc ccatgtcacc ctttatcgcc tcaggcaggc ccgg
//
LOCUS       DAOQNZ010000617         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28129_length_2327_cov_3.863996, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000617 DAOQNZ010000000
VERSION     DAOQNZ010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28129_length_2327_cov_3.863996"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="PKW73_11275"
     CDS             complement(<1..1403)
                     /locus_tag="PKW73_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73914.1"
                     /translation="MQNHLRKPISIICLALTLSLNLAYSAGAVGKNVKTSPARAAGVR
                     AATASLQSRIDAGTMTLMNSGDWAAVAAHIEKLLAGKLPEKLSPAARLKRSYLQAWLS
                     FAYMYVGKPAKTKELLTQIEAEKAKAETQVLKSDVARQSDCFTVVKAMDEVAQGHFDE
                     AGKLLQAGSADAASDCLYNFALACVAGKKGQAQVACDYSRKAFEADRRFAWGLRTIAF
                     LEQKWLKDNRAAEAVLIETLAVEPRISEARDMLVDVKLSRNDFDGAVAAAQTGIKLSP
                     KSAAAHFRLSQIYTQQWRLKESLKELDRAITLDGSSAKYFRNRATVKKLAEDLPGALS
                     DQTKAVELSKDKGFELAELANLNLLAGNTNKAIDCLKEALTSDPENAAARERLYKLLV
                     TEKRYSDLASEYKEQLTRHPKNAALHLGYANVLLALNEDEKAIEEFKEAANLNQTDPS
                     PHRSLGAYYIQKRKFSQA"
     gene            complement(1405..>2327)
                     /locus_tag="PKW73_11280"
     CDS             complement(1405..>2327)
                     /locus_tag="PKW73_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73915.1"
                     /translation="QVAQERKFVCPHVDDSQTLHIKQGRHPVLEKILPMGKYVSNDVR
                     LQGSADDHQLIILTGPNMAGKSSYLRQVALIVILAQMGSFVPAKEAHVGLVDRVFTRI
                     GAVDDLTQGQSTFLVEMSETTQCCLSATKRSLILLDEVGRGTSTYDGVAIAWSVAEYL
                     ARDVRARTIFATHYHELNGLAGFFPQIVNYQVLVKEFEGKVEFIRTVAPGGASRSFGV
                     QVARMAGLPGTIIDRAQYLINQMEKKGVASKILDGPRLRNIPMEEVMQLSLFESEGDG
                     ANPAAKSASKKNSESDRKIVSGADPAAVKV"
BASE COUNT          500 a          642 c          637 g          548 t
ORIGIN      
        1 gcctgtgaaa acttgcgctt ttgtatgtag taggcgccca gacttctatg gggactgggg
       61 tcggtctgat tgaggttggc cgcttccttg aactcttcaa tggccttttc gtcctcattg
      121 agggccagaa gcacattggc atagcccaga tgcagggcgg cattcttggg atggcgagtc
      181 agctgctctt tgtattcact ggccagatcg ctataacgct tttcggtcac cagaagcttg
      241 taaagtcgct cgcgggcggc tgcgttttcg gggtcactgg tgagcgcttc cttcaaacag
      301 tctatggctt tgttggtatt gccggccaac aaattcaaat tagccagttc ggccagctca
      361 aagcctttgt ctttcgacaa ttccacagcc ttggtctgat cgctcaaggc gccgggaaga
      421 tcttcagcca gctttttgac ggtggcacgg ttgcggaagt acttggccga actgccatca
      481 agggtgatgg cacgatcaag ctctttgagg gactccttca aacgccactg ctgcgtgtaa
      541 atctgggaga gtcggaagtg ggcggcagca ctcttgggac tgagcttgat gccggtctgg
      601 gcagcggcga cagcgccgtc gaagtcgtta cgggaaagct ttacgtcgac cagcatgtcc
      661 cgcgcttcac ttatacgcgg ctccacggca agggtctcaa taagcacggc ttcagcggca
      721 cgattatctt tgagccactt ttgctcaaga aaagctatgg ttctgagccc ccaggcaaag
      781 cgccgatcgg cttcaaaggc tttgcgagaa tagtcgcagg ccacctgcgc ctgtcctttc
      841 ttgccggcga cacaggccag ggcaaaatta tagagacaat cggaagcggc atccgcactg
      901 ccggcttgca ggagtttgcc ggcttcgtca aaatgccctt gagccacttc gtccatggcc
      961 ttcacaacag tgaagcaatc actctgcctg gcaacatcgc tcttcagcac ttgcgtctcg
     1021 gccttagcct tctctgcttc tatctgcgtg agcagttcct ttgtcttggc cggcttgccc
     1081 acatacatat aagcaaaact gagccaggcc tgcaggtaac tgcgcttcag acgagcggcc
     1141 ggtgacagtt tctccggcag tttgcctgca agcagttttt cgatatgtgc cgccacagcg
     1201 gcccaatcac cactgttcat caaggtcatg gtgccggcat cgattcgact ctgcaaggag
     1261 gcggtggcgg cgcgaactcc ggcagccctg gccggcgatg ttttgacatt tttgccgaca
     1321 gccccggcac tgtatgccag attgaggctg agagtgagcg ccagacaaat tatggaaatg
     1381 ggctttctga gatgattctg cattttatac cttgacagca gcggggtcag caccggaaac
     1441 aattttacga tcagattcgg agttcttctt acttgcgctc ttcgcagccg ggttggcacc
     1501 gtccccctcg gattcaaaaa gcgataattg cataacttct tccatgggaa tgttgcgcag
     1561 tctcggtccg tcgagaattt tcgaggcaac gcccttcttc tccatctggt ttatcagata
     1621 ttgagcccgg tcaataatgg tgccgggcaa gccggccata cgtgccacct gcacgccgaa
     1681 gctacgactg gctccaccgg gagccactgt gcgaatgaat tctactttgc cctcgaattc
     1741 tttcaccagc acctgatagt tgactatctg ggggaagaag ccggccagcc cgttcaactc
     1801 atgatagtga gtggcaaaaa tggtgcgggc ccgcacatca cgggccagat attcggccac
     1861 cgaccaggcg atggccacac cgtcataggt gctggtgcca cgacccactt cgtcgagcaa
     1921 gatgagcgaa cgcttggtag cggaaagaca gcactgagtt gtttcggaca tttccaccag
     1981 gaatgtcgat tgcccctgag tcaagtcgtc tacggcaccg atacgggtaa agacccggtc
     2041 aaccagaccc acatgggctt ctttggccgg tacgaaagag cccatttggg ccaggatcac
     2101 tatcaaagcc acctgccgca aatagctgga cttaccggcc atattgggac cggtcaggat
     2161 gatcagttga tgatcgtcgg cggaaccttg caaacgcaca tcgttggaaa catatttgcc
     2221 catgggcagg atcttctcaa gaacagggtg ccgcccctgt ttgatatgaa gagtctggga
     2281 atcatctacg tggggacaga cgaacttacg ctcctgcgcc acctggg
//
LOCUS       DAOQNZ010000618         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28180_length_2325_cov_2.962115, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000618 DAOQNZ010000000
VERSION     DAOQNZ010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28180_length_2325_cov_2.962115"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKW73_11285"
     CDS             complement(<1..365)
                     /locus_tag="PKW73_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNA73916.1"
                     /translation="MPAQYSAEEILDLTQGRMAQGMVPDQAGCVATDTRADLNGAWFV
                     ALTGKTFDGHDFIGDAFCSGALGCIVEDRGSYPIASTSFPLIAVDDTEEALACLVANW
                     RKRVRKKITLVTPASSHLPT"
     gene            complement(381..590)
                     /locus_tag="PKW73_11290"
     CDS             complement(381..590)
                     /locus_tag="PKW73_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73917.1"
                     /translation="MTASTIVPPAPVEEETATGTANSVAERTQRTTGSFATRLLQKAL
                     DDRKKTDPNLKVPDLAATATIKTDV"
     gene            827..1930
                     /locus_tag="PKW73_11295"
     CDS             827..1930
                     /locus_tag="PKW73_11295"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNA73918.1"
                     /translation="MIKTLNNPVSKDFVASVARANSEFSLWQKRRHLLNTDDLSLPEL
                     SLELALTHYYKEHRLHSLPPLTTLSDCTVATLFYENSTRTRSSFDLAARKMGATVLHL
                     DVKSSSVAKGESLLDTAMNLCAMGVNAIVMRHSQSGAPDELAEQANQVLARSKDPARV
                     KNLAVINAGDGAKSHPSQGLLDLYTMLSELELLPFLDLSGKLQNPLQPHCLKGKKIAI
                     IGDILHSRVARSNFWLLKNLGAEVHFAGPPSLLPQDFADKHPGAILHHSLKPAVSDAD
                     FIITLRIQMERQEQGLTASLDDYKEGFQVNHERIKWAAKTVKVLHPGPINRGIEITES
                     LADDEEYSRILSQVTNGVLTRMAVLTLLLFQHD"
     gene            1942..>2325
                     /locus_tag="PKW73_11300"
     CDS             1942..>2325
                     /locus_tag="PKW73_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73919.1"
                     /translation="MENEKATAGKEPNESRSRASLWHRTRRYHIIIFWAMLALGVLYT
                     PLQHSFTLGRYQHWGLSITCLGIGYLLQVIWSWRDYTKWAKISYLSTACYFLFVGFTF
                     YANPWLDARMSLQTEEQMGRRSLMLV"
BASE COUNT          548 a          692 c          584 g          501 t
ORIGIN      
        1 gtcggcaaat gactggaggc aggagtgacc agggtaatct tcttgcgcac gcgtttgcgc
       61 cagtttgcca ccaggcaggc cagcgcctcc tctgtatcgt ccacggcaat caaggggaag
      121 cttgtgctgg caatgggata actgccgcga tcttccacaa tacagccgag agccccactg
      181 caaaaggcat cgccgatgaa atcatgccca tcgaaggtct tgccggtcaa agccacaaac
      241 caggcgccgt tcaaatcggc cctggtatcg gtagccacac aacctgcctg atcgggaacc
      301 atgccctgag ccattctgcc ctgggtcagg tcgaggattt cctctgccga atactgagct
      361 ggcaatttat cgctttcaaa ctagacgtcg gtcttaattg tagcggttgc ggccagatcg
      421 ggaaccttga gattgggatc agttttcttg cggtcatcaa gcgctttctg caggagcctt
      481 gtggcaaagc tgccggttgt gcgctgggtt ctttcagcca ccgaattagc agtaccagtg
      541 gcagtttctt cttccaccgg agccggcggg acgatagtgc ttgcagtcat gattccctcc
      601 cggggtgcgg ctattagtgg atctcgctca gaaaactcgg acgccagcca gagatgttca
      661 ccttctgaaa atcagaggga aactcaacaa tgccagatac tagctgacaa cctatctatt
      721 ggcaacccca aagcgctcat aataagctct ttttaaaact tcgtgccggt tgcacttccg
      781 gcatgtcaac gttgacagga gcgtttgata atcacataat tcgcacatga taaaaacact
      841 gaacaatcct gtttccaagg actttgtcgc ctcagtggcg cgcgccaact ccgaattcag
      901 cttgtggcag aaacgccggc acctgctcaa caccgatgac ctcagcttgc cggagctgtc
      961 tctagaactg gcactcactc actattacaa agagcaccgg ctgcacagcc tgccaccact
     1021 gacgacacta agtgactgca ccgtcgccac cctcttttac gaaaattcca cccgcacccg
     1081 ctcatccttc gatctggccg ccagaaagat gggagccacg gttctgcacc tggatgtaaa
     1141 atcctcttcc gtagccaagg gcgagagtct cctggatacg gccatgaatc tatgcgccat
     1201 gggcgtcaat gccatcgtca tgcgccacag tcagtcgggc gcaccggacg aattggcaga
     1261 gcaagccaat caggtcctgg ccagatcaaa agacccggcg agagtaaaaa atctagcggt
     1321 gataaatgcc ggagacggcg ccaagtctca tccctcgcaa ggtttactcg acctctacac
     1381 catgctcagc gagctggagc tattgccatt cctcgatcta tcgggcaagc tacaaaaccc
     1441 attgcagccc cactgtctca agggcaagaa aatagccatt atcggcgaca tcttgcacag
     1501 ccgggtagcc aggtccaact tctggcttct aaaaaatctg ggagcggagg tgcattttgc
     1561 cggtccgccc tcccttttgc cacaagactt cgccgacaag catccgggcg ccatcctgca
     1621 ccatagcctc aaaccggcag tgagcgatgc cgacttcatc atcaccttgc gcatccagat
     1681 ggaacgacaa gaacaaggct tgacagccag cctggacgac tataaggaag gcttccaggt
     1741 caaccacgaa cgcatcaagt gggcggcaaa aacagtgaaa gttctgcacc ctggtcccat
     1801 caaccgcggc atcgagatca ccgagagtct ggccgacgac gaagagtatt cccgcatcct
     1861 cagccaggtg acaaatggcg ttttgactcg tatggcagtc ctgaccctgc tactctttca
     1921 acacgactag gcgggctccc tatggaaaac gagaaggcca cggcaggcaa ggagccgaat
     1981 gagagccgtt ctcgggcctc gctctggcat cgtacccggc gctatcacat cataattttc
     2041 tgggccatgc tggctctcgg ggtgctttac acccccctgc agcactcatt cacccttggc
     2101 cgctaccaac actggggtct gtctattact tgcctgggca tcggctactt gctgcaagtg
     2161 atctggtcct ggcgcgacta taccaagtgg gccaaaatca gctatttgag tacggcctgc
     2221 tacttccttt tcgtcggctt cactttctat gccaatccct ggttggatgc cagaatgtcc
     2281 ttgcaaacgg aagagcaaat ggggcgccgc tccctcatgc tggtg
//
LOCUS       DAOQNZ010000619         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28232_length_2322_cov_4.398324, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000619 DAOQNZ010000000
VERSION     DAOQNZ010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28232_length_2322_cov_4.398324"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PKW73_11305"
     CDS             complement(<1..756)
                     /locus_tag="PKW73_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73920.1"
                     /translation="MPRVGPKTQAASLAALLLLASTGFHDSALALGLDLEKIKNDLLK
                     GKGSRGGRQEQIAQAEEQALTLISSGSGESLAMARQLLETSSQNGLDSARMHLYLGRI
                     YEKAGQNDKAVMAYIEALILSEGGGIDALSAREELSALYMRQGNYDEAGGQLKKILEV
                     SPQDIKSRGNLGICFLQLGYAAASLDEFKRVLQVDPNNFVALYNSGLAHTMEREPEKA
                     AAAFEKAVASGEKSGEPLLPMAYLGLARAYQARG"
     gene            897..2174
                     /locus_tag="PKW73_11310"
     CDS             897..2174
                     /locus_tag="PKW73_11310"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNA73921.1"
                     /translation="MEFHWAALFQSQEAIIWKVIVPPILYFAHGYLATWMAVAALFRP
                     YDAYFVPFTKIQLPLTPGIFPKRRAKLAQAVAGTITDTLLTPQDIKGQVEHLLTEDNI
                     RLTIGLLVDSVLKEFRDTTKLHRLASDIAELSPTLLEHFVASSIESLEAGKDAKIATI
                     TEKVFDQVILTTRISLEQASEFASRILEAFATPPKVRAGLITLLSPSNISALEESISA
                     HASGPYKLLARIIGVKRVCYEWRNFLEKEPEEADKIIQDMIKRFGIKEQIALQIANFD
                     LRSMPLQTVNKFKANMVTFVEAFLIEHKSDIIASVRKVEGEAMASVRQAIVGFNPESI
                     PEPWLMRAKQDLSQFFYSYLKRELGELLEKAIPKLGMYNVIAQKIDLFSAQQLENLVK
                     RICKQELQALEWFGGFIGLMLGFVQILVNAIAP"
BASE COUNT          572 a          652 c          547 g          551 t
ORIGIN      
        1 ccctctggcc tggtaagccc gggcaagtcc cagataggcc atcggcaaaa gcggctctcc
       61 cgatttttcg ccgctggcca cggctttttc aaaggcggcg gccgctttct ccggctctct
      121 ttccatggta tgggccagtc cggagttgta gagggccaca aagttattcg gatccacctg
      181 caagacacgc ttgaactcat cgaggctggc ggcagcgtac cccaactgca agaaacaaat
      241 gcccaggttg ccccttgact tgatgtcctg aggactgact tccagaatct tcttgagctg
      301 gccgccggcc tcatcgtaat tgccctgcct catatataga gcggacaatt cctccctggc
      361 actcaaagca tcgataccac cgccctcgga aagaataagc gcctcgatat aggccatgac
      421 ggctttgtca ttctgtccgg ctttctcgta gatacgtccc agataaaggt gcatgcgggc
      481 actatcgaga ccgttctgag aagaggtctc caatagttgc ctggccatag ccagactctc
      541 gccgctaccg gagctgatga gagtgagagc ctgttcttcc gcctgtgcta tctgctcttg
      601 tctgccgcct cttgacccct tacctttgag gaggtcattc ttgatcttct ccagatccag
      661 ccccagggcc aaggcgctat catggaaacc cgtagaggct aacagcagta aagccgcaag
      721 cgaagctgct tgtgtttttg gaccgactcg aggcaattta tcctcctcag ctttaaccgc
      781 atcataacca accggcggac acgctaatta taagaggagg tcaagcaggt gcttatgctt
      841 atagaataag tcttgtctta cttaatgaat acgtaaaaca accaggaagc acggaaatgg
      901 aatttcactg ggcggctctt tttcaaagtc aggaagcgat catctggaaa gtgatcgtgc
      961 ctcctatcct ttattttgcc cacggctacc tggctacctg gatggcggta gcagctctct
     1021 ttcgccctta tgatgcctat ttcgtgccct tcacaaaaat ccagcttcct ctgacgcccg
     1081 ggatcttccc caaacgcaga gccaagctgg cccaggccgt ggccggcaca atcacggata
     1141 cgctcctcac cccgcaggac atcaaaggtc aggtcgagca cctgctcact gaagataata
     1201 ttcgactcac tattggtctc cttgtagatt cggttcttaa agagtttcgc gataccacca
     1261 aactacatag actggcttcc gatatagccg aactcagtcc caccctcctc gagcatttcg
     1321 tggcctcttc catcgaatcg ctggaggcgg gcaaagatgc caaaattgcc acaattaccg
     1381 agaaggtctt cgaccaggta attctgacaa cccgcatttc tcttgagcag gcaagcgaat
     1441 tcgcctcgcg catcctggaa gcctttgcga caccacccaa ggtgcgagcc ggtttgatta
     1501 cccttttgag tccctccaat atcagcgccc tcgaggagtc aattagcgct catgccagcg
     1561 gtccttacaa gcttttagcc agaatcatcg gcgtcaagcg cgtctgctat gaatggcgca
     1621 atttcctgga aaaagaaccg gaagaagcag acaaaatcat ccaggacatg atcaagcgct
     1681 tcggaatcaa agagcaaatt gctctgcaga ttgccaattt cgacctgcgt tctatgcccc
     1741 tgcaaaccgt aaacaaattc aaagccaaca tggtcacctt tgtggaagcc ttcttaattg
     1801 aacacaaaag cgatatcatc gcctcggtgc gaaaagtgga aggcgaagcc atggccagcg
     1861 tcaggcaagc catagtcggc ttcaacccgg aatcgattcc ggaaccctgg ctcatgagag
     1921 ccaagcagga tctgtctcag tttttctact cttacttgaa gcgggagcta ggtgagttat
     1981 tggaaaaagc tataccgaag ctgggtatgt ataacgtgat tgcccagaag attgatctat
     2041 tttcggctca acaactggaa aacctggtca aacgtatttg caaacaagag ttacaagccc
     2101 tggaatggtt tgggggattt atcggactga tgctcggttt tgtacagatt cttgtcaatg
     2161 ccattgcgcc ctgaggtctt ttaatgaaaa gaccccacaa gagccccgca ccaaaactga
     2221 taccactgcc tggggcctca cgttacccgc tctatcgcat caagattcca ccaaatttca
     2281 aagatttgct ggtcggagcc accctgggca accgttactg gg
//
LOCUS       DAOQNZ010000620         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28321_length_2319_cov_3.149735, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000620 DAOQNZ010000000
VERSION     DAOQNZ010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28321_length_2319_cov_3.149735"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PKW73_11315"
     CDS             <1..264
                     /locus_tag="PKW73_11315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNA73922.1"
                     /translation="PLMSLPNVPVVIQLNTRGGSAAVMGRTACHEFGHAFGLQHSKST
                     SDIMFDGRAGMNVANTLSDSDILTIRALYQLPVDIWLVGNRRP"
     gene            complement(265..1263)
                     /locus_tag="PKW73_11320"
     CDS             complement(265..1263)
                     /locus_tag="PKW73_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNA73923.1"
                     /translation="MTDTKFQSAFASLSPGSICLRLRLVLLGPLLSLSLFAGSAQGQE
                     TLKLAVKPERPYLQPDLNLFTTGLTLKVAEGGEKFPIIDNIDRGAPAYSLGLRSGDRL
                     ISVNGEIAAFMGFDELRGALTQKNMQVLTLEVEKQDGKTQSYRIPMIQAGQLQDSKMA
                     RLLADQRPHYPVTRTHISGGFAPIDLPSFLHHEAMEGPLVVEFYQGHADQRLKEAIAA
                     LNRQILAGQSSGAETQEQIISLISLSLDDADTRPLRTHFRITRAPSYIFIGTRESFIK
                     DYIDVVRHELSEADLEERLAEASLLHSRKQNMLPLLYPQLAKRWKEITNQEIDRAQ"
     gene            1358..1693
                     /locus_tag="PKW73_11325"
     CDS             1358..1693
                     /locus_tag="PKW73_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNA73924.1"
                     /translation="MPQFRVNFSRCHFLLSLLVLFAVSISFCLSRVGAAEPAGRSLLK
                     STRVSIEKAEEMAMAKFPEGSVEEIELDRDDSPLVWKIDLKIQPRTKAEVEIDANSGE
                     ILKARSRKW"
     gene            1817..>2319
                     /locus_tag="PKW73_11330"
     CDS             1817..>2319
                     /locus_tag="PKW73_11330"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73925.1"
                     /translation="MTYLPPRFFGSGTPLWCRTVRFALVLAFIIPSMQMPLVAAAAHS
                     EAAEGQAKEKPPSWHRETELGRMALLKGEFDQALEHFQSALSLSQPLPASDSRKAISL
                     SNLSLIYKHKGDLSQAESYSRQALKAMNAAGMKDASMFSVELNNLAGVLSEEGKYAES
                     IELYDSSL"
BASE COUNT          487 a          602 c          625 g          605 t
ORIGIN      
        1 cctctaatgt ccctaccaaa cgtaccggta gtgatccagc tcaacactag aggcggcagt
       61 gctgctgtca tgggtcgaac tgcctgtcat gaattcggcc acgcctttgg tctgcagcat
      121 tccaaaagca cttccgacat catgttcgat ggccgggccg gtatgaatgt ggctaatacc
      181 ttgtcggaca gtgatatcct caccattcga gcgctctatc agttgcccgt agacatctgg
      241 cttgtaggca atcgccgccc ctgatcactg ggctcggtcg atttcttgat tggtgatttc
      301 tttccaacgt ttagccagtt ggggatagag caggggcagc atgttctgtt tgcggctgtg
      361 caggagactg gcttccgcca atctctcctc caggtctgcc tcgctcagtt cgtgtctgac
      421 cacatcgata tagtccttga tgaagctttc tcttgtgcca atgaagatat agctcggtgc
      481 ccgcgtgatt cgaaaatggg ttcgcagcgg gcgggtatcg gcgtcgtcaa gggacaggga
      541 gataaggctt attatttgtt cctgagtttc tgcaccgcta ctctgtccag ccagtatctg
      601 tcggttcaaa gcggcgatgg cttccttcag cctttgatcg gcatgaccct ggtagaactc
      661 caccacaagc ggtccttcca tagcttcatg gtgcaagaaa cttggcaggt cgatgggggc
      721 aaagcctccc gagatatgag ttctggttac cggatagtgg ggtctttgat cggccagcag
      781 tctggccatt tttgaatctt gtaattgccc ggcctggatc atgggaatcc ggtagctctg
      841 ggttttcccg tcttgcttct ctacttctaa agtgagaacc tgcatgttct tttgcgtgag
      901 ggcgccgcgt agttcatcaa agcccatgaa ggcggctatt tccccattta cactgatgag
      961 cctgtcgcca ctgcgcagac ccaggctgta ggccggtgcg cccctgtcga tattgtcgat
     1021 gatgggaaat ttctcgccgc cttctgctac tttcaaagtg agcccggtgg taaacaagtt
     1081 gagatcaggc tgaagataag gacgttccgg tttgacggcc agtttgagag tctcctgccc
     1141 ctgagcgctg ccggcaaata aggataacga cagcaaaggc cccagcagca ccagtctcag
     1201 tctcagacaa attgagccgg gagaaaggct tgcaaacgct gattggaact tggtatcagt
     1261 caagggcggc tcttttcggc acctattttg gtgcctgttt cggtatctgt ttcaggtaga
     1321 atcctacatt atatttgtac atgccccagg agcatttatg cctcagttca gagttaattt
     1381 cagccgctgc cactttctgc tttccctctt ggttctgttt gcggtaagca tctccttctg
     1441 cttgagccgg gtaggtgcag cggaacctgc cggccgaagc ctgcttaagt ccaccagagt
     1501 ttccatcgaa aaggcggaag aaatggccat ggctaagttt ccggaaggtt cggtggagga
     1561 aatcgagttg gatcgcgatg acagccccct ggtctggaaa atcgacctga aaatccagcc
     1621 tcgcaccaag gcggaagtgg aaatagatgc caattccggc gagatactca aggcccgcag
     1681 tcgtaagtgg tagcggaggg ctcattgggg tctgtataag ttaagtctcg ttttgacgga
     1741 ccggcgctct catgcctgct tttactgcta tgcttcaatc taagttcggt cggcaatgct
     1801 gcaggttgtg caaaagttga cttaccttcc cccgcgattt ttcggtagtg gcactccttt
     1861 gtggtgccgc acagtgcggt ttgctctcgt actggctttc ataattcctt ccatgcaaat
     1921 gcccttagta gctgccgctg cgcattccga ggctgctgaa ggacaggcta aggaaaagcc
     1981 tccttcctgg caccgggaga ccgaacttgg tcgcatggcg cttctgaagg gagagtttga
     2041 ccaagcgttg gagcattttc agagtgccct ctccctcagc cagccgctgc ccgcttccga
     2101 cagtcgcaaa gccatatcat tgagcaatct atcgctcatc tacaagcaca agggcgatct
     2161 aagccaggct gaaagttact cccggcaggc tctcaaggct atgaacgcgg ccggtatgaa
     2221 agatgcatcc atgtttagcg tggaactcaa caatcttgcc ggggttttga gtgaagaagg
     2281 taagtatgcc gagtccatcg agttgtacga tagttccct
//
LOCUS       DAOQNZ010000621         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28409_length_2315_cov_4.629646, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000621 DAOQNZ010000000
VERSION     DAOQNZ010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28409_length_2315_cov_4.629646"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKW73_11335"
     CDS             complement(<1..210)
                     /locus_tag="PKW73_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73926.1"
                     /translation="MSNSYRKNPFIGNCSNSDKPGKVNANRTLRTHVRQALRTCVDFE
                     NLLLPALREVSNVWDFPKDGKHRLNT"
     gene            complement(803..1801)
                     /locus_tag="PKW73_11340"
     CDS             complement(803..1801)
                     /locus_tag="PKW73_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73927.1"
                     /translation="MFRVFDKKVSLLAAALYSAQAVLPALALDMRDSGVNTVDVPKQI
                     LKSGINLSNASLSPNSQQLADQLNLTPILVRIQELRAITASQKGETSLEALSNRISLQ
                     EQLESARSILLQTAFEADFVTSEIQAEQNVYASVLSTFQSNRDKIVARTNAASFITNG
                     ILWAVSCGLAIPTYKYPGYSVQSGITGVIAGIVPSVASMYALKAYEGRHKTSEQEPNM
                     LARIFDYPTNPEIDYPASIWKFLTSVPASDGGTRTRKDQLIDRWISDKNIPGFTDSKS
                     KKQLDAITASISQKKGLCIDNLNARLVMLEQLEGEVMKLKRLLLELSMAVNGSKTI"
     gene            complement(1782..2120)
                     /locus_tag="PKW73_11345"
     CDS             complement(1782..2120)
                     /locus_tag="PKW73_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73928.1"
                     /translation="MGLSLILFLCFVAWIGGHLLDRLLTMLQANMTELFESSSRFGPA
                     KKTRKTTPSNVIILPALASNPKKLDGKGQVRLSSYTRGRVVKRSETKKLSRQAEKGNL
                     TGDPDVQGVR"
BASE COUNT          490 a          548 c          667 g          610 t
ORIGIN      
        1 agtgttgagg cggtgcttgc cgtccttggg gaagtcccag acattgctca cctcgcgcag
       61 ggcggggaga agcagattct caaagtcgac gcaggtgcgc agagcctgac gcacgtgggt
      121 gcgcagggtg cggttggcgt tgaccttgcc gggcttgtcg ctgttagagc agttgccgat
      181 gaaggggttc ttgcgatagg aattggacat ggtgaggtgc tcctttggaa gcactccacc
      241 gagctttggt cgaaaacctc acagtgtccg gctgtgaacg cttttcaaaa gcgctgacat
      301 gattttcgac cggcgcggta aagtgctagt agacagcacc gtgccgcgtc tttgccaact
      361 gaaggaacat acttgcctcc ttagttttga gcattacgct gttgtggttg tttgtcattg
      421 ccgctctcaa agggatgatt accagctcac agcttctctg ggctgcttgc agggctttat
      481 ctctactgat gagctggttg gtgagtttaa agggagctta agggtcctta tgagataggg
      541 cgaaaatgac tcctctacct tagaggcagg ctcccctggg aaccagtgtc tggggattta
      601 gttgtaaggg tgttaattaa acttgcgcta atgaattgct tgggtacact gataatgccc
      661 cggaccggct cttgaagcca ggcaggggag gcggtttcgt tcttgcaacc ggcagtctcc
      721 ccggaaaaac agttatccgg ccccggttgg cgcagctagg caaacctgcg gaattctccg
      781 ctgttttctc cggcagtttt gcctagatgg tcttactgcc gtttacggcc atggaaagtt
      841 ccagtagcag gcgctttagc ttcatcactt ccccttccaa ctgttcaagc attaccagcc
      901 tggcgttcag gttgtctatg cagagcccct tcttttgaga aatggaagca gttatggcat
      961 cgagctgttt cttcgacttg gaatcggtga aaccggggat gttcttgtcg ctgatccagc
     1021 ggtcgataag ttggtctttg cgagtgcgtg tgccgccgtc gctggccggt accgaggtga
     1081 gaaacttcca gatggaagcc gggtagtcga tctccggatt ggtgggatag tcgaaaattc
     1141 tggccaacat attgggctct tgctccgatg tcttatggcg cccttcatag gccttgaggg
     1201 cgtacatgga agctacggag gggacaattc cggcgatgac gccggttatg cccgactgta
     1261 cggaatagcc tgggtacttg taggtgggaa tggccagtcc gcaagaaacc gcccagagta
     1321 tgccgtttgt gataaagctg gcggcattgg tgcgggctac gattttgtcg cggttactct
     1381 ggaaggtgga gagaaccgaa gcgtagacgt tctgctcggc ttgaatttcg gaggtgacaa
     1441 aatcagcctc gaaagctgtt tgcagtagga tactgcgggc gctttccagt tgctcttgca
     1501 gggaaatgcg attggagagg gcctccaggc tggtttcgcc cttctgggag gcggtgatgg
     1561 cgcgcaactc ctgaatcctt accagaatgg gggtgagatt gagttgatcg gccagttgct
     1621 ggctattggg agaaaggctt gcattactca gattgatgcc gcttttcagg atttgcttgg
     1681 gtacatcgac ggtgttgacc ccactgtctc gcatatccag tgcaagagcg gggagcaccg
     1741 cctgtgccga atagagcgcc gcagccagca gacttacttt tttatcgaac accctgaaca
     1801 tctggatctc ctgttagatt gcctttctcg gcttgccggc tcagcttttt cgtctcgctt
     1861 cttttcacca ctctgcctct ggtgtagctg ctcaatcgaa cctgaccttt gccgtccagc
     1921 ttttttggat tgcttgccag ggcaggcaga attataacgt ttgagggcgt agtctttctc
     1981 gtcttcttag ccggaccaaa tcttgaagag ctttcaaata gttcggtcat gttggcttgc
     2041 agcatggtca gtaagcggtc taaaagatgc ccaccaatcc aggccacaaa gcaaagaaaa
     2101 agaattagag atagacccat ttgctttcac cagctacctg agtctattaa cggctatgtt
     2161 gcgtcaagat gttatcaacg aaagtgctga tgacatcaaa atggaatcaa gaaaaggcct
     2221 cgattctgaa aggactgatg attgctatcc tttagagata ctttttgctg acgcccagat
     2281 ccagggcgaa gcggtagccg atcagcttgt agatc
//
LOCUS       DAOQNZ010000622         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28444_length_2314_cov_4.100044, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000622 DAOQNZ010000000
VERSION     DAOQNZ010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28444_length_2314_cov_4.100044"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..848)
                     /locus_tag="PKW73_11350"
     CDS             complement(105..848)
                     /locus_tag="PKW73_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73929.1"
                     /translation="MPPVFSFCDLDDTLFRSGRKAAADDLARAVAFSSKGLPISFQSR
                     EQAALYSWLAASSRLIVTTGRSSKWLSRITLPFNDYAICSFGGVILSPDGEPQPDWYA
                     YIQSEVEKTRADLLALKHVCEAEASRLCFDVRVKVVKDKGLDLHLSIKHNKRSQNETT
                     SLAALLREQLPAGWWMHLNGRNMALCPPFLGKDKAVAWFLDHLAGTYGLVLGVGDSLS
                     DLSYMGLGHGAVIPVKPKSQIFATLSDLA"
     gene            complement(850..2052)
                     /locus_tag="PKW73_11355"
     CDS             complement(850..2052)
                     /locus_tag="PKW73_11355"
                     /inference="COORDINATES: protein motif:HMM:NF026947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HNA73930.1"
                     /translation="MSLINQETISPGEQDVTHAEAGLHRVELPTGVISVSVHEPLLPL
                     HSLCGFAARQNPKRGFLFVSKVLGKHIPVRPSLMLTVQERLTSQIPADLPGPVVVIGM
                     AETATCLGQGVFEAYKAMTGRDDLLFIPSTRYRLNHPVALQFLEEHCHAADHIMYLPE
                     NERARELFEHARSLILVDDEASTGKTFVNLATAFCRRINSIERLVTAVITDWRGPERT
                     ERSQADMPVQCNTVALLKGLYQFQAAASLQAMQMPHAVGNDACKNHLLARNHGRLGTT
                     ECFQLPGQLVSQAKALVEEAAGKPVLVLGTGEFSFPPFRLALYMEELGADVYFQTTTR
                     SPIIADAGTAIACGFSFADNYEDGISNYLYNVEPKQYGLVVLAHETPAYTIASRLRDE
                     LDARLVEI"
BASE COUNT          561 a          633 c          637 g          483 t
ORIGIN      
        1 aatactaaag gttgaacagg tgattttgac atttgtttct ccggtttaaa taaaaatgaa
       61 aggcacccgg ccgcagaagc ggctgtacaa gcgcccaaat gtttttaagc aaggtccgac
      121 aacgtcgcaa aaatctgcga cttcggcttc acaggtatta cggcaccatg cccgagcccc
      181 atataactga ggtccgaaag gctatcgccc acacccaaga cgagaccgta ggtgccggca
      241 agatggtcga ggaaccaggc aacagccttg tccttaccaa gaaaaggcgg acagagtgcc
      301 atgttccgcc cattcaagtg catccaccaa ccggccggca actgctcccg caagagcgca
      361 gccaggctgg tggtttcgtt ctgagaccgc ttgttgtgct tgatggaaag atgcagatca
      421 agacccttgt ctttcaccac cttaacgcgc acatcgaagc acagacggct agcttccgct
      481 tcacagacgt gcttgagggc aagcaagtcg gctctggttt tctcaacctc agactgaata
      541 taggcatacc agtcgggctg aggttctcca tcgggagaca gaatcacacc gccaaaggaa
      601 cagatggcgt agtcattgaa cggcaatgtg atgcgagaaa gccatttgga actacgcccg
      661 gttgtgacaa tgagccggct gcttgctgcc agccaggaat aaagagcggc ctgctccctg
      721 gattgaaagc ttatgggtag acctttggag ctaaaggcca ccgctcttgc caggtcgtcc
      781 gcagcagcct tgcgcccact cctaaagagc gtgtcatcta aatcgcagaa actgaagacg
      841 ggcggcatgc tatatctcca ccagcctggc atcgagttca tcacgaagtc ttgatgcgat
      901 ggtgtaagcg ggcgtctcat gagccagtac cactaatccg tattgcttgg gctccacgtt
      961 gtagaggtag ttactgattc catcctcgta gttgtcggcg aaagaaaatc cgcaggcgat
     1021 tgcagtgccc gcatcggcaa tgatgggtga gcgggtagtg gtctggaaat acacatccgc
     1081 gccaagctct tccatataga gcgccaggcg gaagggcggg aaggaaaact caccggtacc
     1141 gagcaccagc accggttttc ccgcagcctc ctcaaccagc gccttagcct gactgaccaa
     1201 ttgccccggc aactgaaagc actcggttgt accgagtcgt ccatgattgc gcgcaagcag
     1261 gtgatttttg caagcatcgt tacccacagc gtgtggcatt tgcatagcct gcaaactggc
     1321 agcagcctgg aactgataca agcccttgag cagagcgact gtattgcact gaacgggcat
     1381 atccgcctgg cttctttcag tacgctccgg tccgcgccaa tcggtaatga cggccgtgac
     1441 cagccgctct atcgaattaa tgcgacggca aaaggctgtg gccagattga cgaaggtctt
     1501 gcctgtggag gcctcatcgt ccaccagaat gagagagcga gcatgctcga acagttccct
     1561 ggctctctcg ttttccggca aatacatgat gtggtcggcg gcgtgacagt gctcttccag
     1621 aaactgcaag gcaacgggat ggttgaggcg atagcgggtg gagggtatga agagcaaatc
     1681 atccctgccg gtcatggcct tgtaagcttc aaagacgccc tgtccaagac aggttgccgt
     1741 ctccgccatg ccgattacca ccaccggtcc gggtaagtcc gctggtatct gactggtgag
     1801 gcgctcttgc acggtgagca ttagcgaggg acgcaccgga atgtgcttgc ccaggacttt
     1861 agagacaaaa aggaaaccgc gtttgggatt ttgccgggcg gcaaatccgc aaaggctatg
     1921 aagtggaagg aggggctcgt gcacggacac cgagattaca ccagtaggta gttctacccg
     1981 gtgtaatccc gcctcggcat gggtgacatc ctgttccccc ggagatatag tctcttgatt
     2041 gatcaaactc atgaattatg tcctccaaac ctaaactccc ggcggctgcc aagaggtgag
     2101 ggccaaaaaa aagaagagcg cgccaaaact gcctgagcag cggcacgctc ttcacctcaa
     2161 aggtaggtca ggctaagcga ggcttagacc agaccaagct ccttgtccaa ctcagcgcga
     2221 actgccgcag ccgaagcttg tttctcgata ttggcaattt cgcggtcggt gtcggaaccc
     2281 tgagccagtt cgtttttggc gcgcgctttc tcca
//
LOCUS       DAOQNZ010000623         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28475_length_2313_cov_3.225864, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000623 DAOQNZ010000000
VERSION     DAOQNZ010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28475_length_2313_cov_3.225864"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..964)
                     /gene="dapA"
                     /locus_tag="PKW73_11360"
     CDS             complement(47..964)
                     /gene="dapA"
                     /locus_tag="PKW73_11360"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNA73931.1"
                     /translation="MIKNDAPLFGRVITAMVTPFKGSEHEIDYAALEKLVEHLLATGT
                     SAIVVAGTTGESPTLEDSEKKDLLKATIKLVNGRAKVIMGTGSNITAKSIKATKEAEA
                     LGADGALVVAPYYNKPSQAGLLAHFGAVAEASDLPIIMYNIPGRTGINMAVETILELA
                     DKYKNIVAVKDSTGNVDQASDIAAKAPETFRIYSGDDNMTLPFLSVGACGVISVASHL
                     VGDEIKSMADKFFAGNLDQARSLHYRLMPLFKGLFAAPNPTCVKYALSLKGLGSEDLR
                     LPLVPLSEAQKATMNSLLSQYDKATPACK"
     gene            complement(978..1823)
                     /gene="dapB"
                     /locus_tag="PKW73_11365"
     CDS             complement(978..1823)
                     /gene="dapB"
                     /locus_tag="PKW73_11365"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNA73932.1"
                     /translation="MSELERMTGKVKVAIAGVNGRMGRAALAALANDEDISIVGAFGR
                     SGAAYVGRSLSEMCAQSAARLEGIKVADSFEAMPAGVKPDVVLDFMVAEASVALAGKC
                     IAAGIRPVIGSSGIAADDVEALRQSAAKAGLGVLIVPNFSLGAVLMMEFARQAARYFA
                     NSEVVEIHHTKKLDAPSGTAMHTLDKMAQASEAGKFNERDVVERELLTGARGALHQSG
                     LRVHSLRLPGLISHQEVFFGAEGELLTIKHDSFNTACFNKGIVMAVKAVGKLKELQIG
                     LETIL"
     gene            complement(1863..>2313)
                     /gene="dut"
                     /locus_tag="PKW73_11370"
     CDS             complement(1863..>2313)
                     /gene="dut"
                     /locus_tag="PKW73_11370"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNA73933.1"
                     /translation="KRLPHCFSLPAYATPGSAGMDLTAAIAEAVTLPAGARRRIPCGI
                     QLEIPPGYEGQVRPRSGLADKAGISLTNCVGTIDSDYRGEVQVLIINHGEKAYTFEPG
                     ERIAQLLILPVPRVEVIEVEDLAESAERGEGGFGSTGKLAIKEAASK"
BASE COUNT          539 a          667 c          575 g          532 t
ORIGIN      
        1 ggttccaatt tagccgaagc agactttctc tgcttcccgt ttttatctac ttgcacgctg
       61 gggtcgcctt atcgtattga gagagtagac tgttcatggt cgccttttgc gcttccgata
      121 gcggcacaag aggcaggcgt agatcttcgc taccaagccc cttgaggctc aaggcatact
      181 tgacacaggt aggattggga gcagcaaaca agcccttgaa gagcggcatc aatcgataat
      241 gcaaagagcg cgcctgatct aaattgccgg caaagaactt atcagccatc gacttgattt
      301 catcaccaac caggtgagag gcaacggaaa taacaccgca agcccctact gagaggaagg
      361 gcagagtcat attatcgtcg ccggagtaaa tgcggaatgt ttccggtgcc ttagcggcga
      421 tatcggaagc ttgatccaca ttgccggtgg agtctttgac ggccacaata ttcttgtact
      481 tatcggccag ttctaaaatg gtttccacag ccatgtttat gcctgtgcga ccgggaatgt
      541 tatacattat gataggcaaa tcgcttgctt cggcaacggc accaaagtgg gcaagcaaac
      601 cggcctggct gggcttattg taatagggcg caacaacgag agcaccgtcg gcccccagtg
      661 cctcagcctc tttagtggct ttaatagact tggcggtaat attggaaccg gtgcccataa
      721 ttactttcgc tctgccgttt accaacttga tggtagcctt gagcaagtcc tttttctcgc
      781 tatcttcaag ggtgggactt tcaccggtgg taccggccac aacgatggca ctggtgccgg
      841 tggcaagcaa atgctccacc agcttctcga gagcagcgta gtctatctca tgctcactgc
      901 ccttgaaggg ggtgaccatg gcggtgataa ctcttccaaa aagcggcgcg tcgttcttaa
      961 tcacttctac ttccccctta gagaatggtc tccagcccaa tttgcaattc tttcagttta
     1021 cccacggctt tcaccgccat gacaataccc ttgttgaaac aagcggtatt gaagctatcg
     1081 tgcttgatgg tcaagagttc accttctgca ccgaagaaga cctcctggtg agaaatgaga
     1141 cctggcagcc gcaggctgtg taccctcagt cccgactggt gcagggcacc gcgtgctccg
     1201 gttaagagtt ctctttctac cacgtctctc tcattgaact tgccggcttc cgaagcctga
     1261 gccatcttat ctagtgtatg catggcggta ccggaaggag catccagctt tttggtatgg
     1321 tggatttcca caacctcgct attggcaaaa tagcgagcag cttgccgggc gaactccatc
     1381 atgagcacgg cacccaggga aaagttaggc acaatcaaga ccccaagccc agctttggca
     1441 gccgactgcc tcagcgcctc cacatcatcc gctgctatac cggagctacc gataaccggt
     1501 ctgataccgg cagcgatgca tttgccggcc agggcaactg aggcttccgc caccataaaa
     1561 tcaagcacca catccggttt gacaccggca ggcatagctt cgaagctgtc ggcgaccttt
     1621 atgccttcca ggcgcgccgc actttgagcg cacatctcgg agagcgatcg acccacatag
     1681 gcagcaccac tgcgcccaaa ggcccccaca atcgagatgt cttcatcgtt ggccagtgca
     1741 gccagggctg cccgccccat ccgtccattg actccggcaa tggcaacctt cacttttccc
     1801 gtcattctct ctaactcaga cactaaactc tcacagataa aaaccgaaaa cggcaattct
     1861 atttacttag acgctgcttc cttgatggcc agtttgcctg tggaaccaaa gccgccttcg
     1921 cctctttcag cgctctcagc caagtcctca acctcaatta cttccactct cggcacaggc
     1981 aaaatcaaaa gctgcgcgat tctctccccc ggctcaaagg tgtaggcctt ctcaccatgg
     2041 ttgataatca gaacctgcac ttcgccgcgg tagtcactat cgatggtgcc aacgcaattg
     2101 gtgagggaaa taccggcttt atcagccagt cccgagcgcg gtctcacctg tccttcatag
     2161 ccgggcggta tctccaattg gataccgcag ggaatgcggc gtctggcacc ggcaggcagg
     2221 gtaacggcct cggcaatggc agcggtcaga tccataccgg ccgaacctgg ggtggcataa
     2281 gcgggcaagg aaaaacaatg gggcaggcgc ttc
//
LOCUS       DAOQNZ010000624         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28500_length_2312_cov_3.486930, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000624 DAOQNZ010000000
VERSION     DAOQNZ010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28500_length_2312_cov_3.486930"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKW73_11375"
     CDS             <1..354
                     /locus_tag="PKW73_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA73934.1"
                     /translation="ELGARLRALLRRPPQILSEVLTAGHISVDGNSRRVLKSGVEIKL
                     LPQEFNLLLFFMRNPNKVFSSEEILDKVWSNEKDTAPDTVRVHINKLRKKIDEEGKTS
                     VLRTVHGSGYILDKH"
     gene            complement(351..1607)
                     /locus_tag="PKW73_11380"
     CDS             complement(351..1607)
                     /locus_tag="PKW73_11380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNA73935.1"
                     /translation="MELLRARLLTITYFTIIAAVLVVPYGELVKRAQTNELAFSTRNK
                     QLYLSDFVQFYTCGKLVLEPSTRTRIYDPAAINKAMPVAVPMEDRIIPAVPFFIVLMA
                     PWALLPVDTSFLVWILLSAICMAGGSLLLLRGWKEATPLACLGFLTAILMAQPSWFTL
                     FIGHACFYQYLFLSLFFFALLHKKDALAGAGMALASFKPQFAIPLLVPALAGRRFILL
                     AWGAAFELGLLALSVFTVGLDNTIHFPALIAKYEHDQSRVVPRMICLRAIIERVLPHQ
                     ATMLLSWALFALSLAMAYYLWRRTMKNQKLWPLAASFTITAGLVFSPHAHVYDAVMMG
                     LVAIMLLPVNGLFGFASLSRTRKLLCTSLMLYPMASWYFFVGISEAGNMIGYPFAVLD
                     LLILLGILAVFKEENRQEEATPKPET"
     gene            1986..>2312
                     /locus_tag="PKW73_11385"
     CDS             1986..>2312
                     /locus_tag="PKW73_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73936.1"
                     /translation="MRANILDDRPGAAIFFPFAAIVIPIILSLFLTITLQNPANAQST
                     VFEERHRVSGWEEPLCAREGNLRHYYWTEIDASSRYRANAINRVAPSGRASYKIISRT
                     AGPNSRP"
BASE COUNT          598 a          560 c          644 g          510 t
ORIGIN      
        1 gaattagggg ccagattgcg ggcactttta cgccgaccgc cgcagatttt gagcgaagtg
       61 ttaacggccg gacacatcag tgtggacggt aattcgcgcc gggttttgaa aagcggagtg
      121 gaaatcaaac ttttgcctca ggagttcaac ttgctccttt tctttatgcg caatcccaat
      181 aaggtattca gctccgagga aattctcgat aaggtatggt cgaacgagaa agacacggca
      241 ccggatacag tgcgggttca tatcaataag ttgcgcaaga aaattgatga agaagggaag
      301 acttcggtct tgcgcaccgt ccacggtagt ggctacatct tagacaaaca ctaggtttcc
      361 ggcttcggag tcgcttcttc ctgtctgttt tcttctttga agacggctag tatgccgagc
      421 agaattaaga ggtctagcac ggcgaaagga taaccgatca tgttgccggc ttcgcttata
      481 ccaacgaaga aataccagct tgccattggg tagagcatca agcttgtgca gagcagcttt
      541 cgggtgcggc tgagagaggc gaagccgaag agcccgttta caggcagcag catgatggcc
      601 acgagcccca tcatgactgc gtcatagaca tgggcatggg gcgaaaaaac aagaccggct
      661 gtaattgtga aggaggccgc caggggccag agcttttggt tcttcatggt gcggcgccac
      721 aggtagtagg ccatcgccag ggaaagcgca aagagagccc aggacaggag catggtggct
      781 tgatggggca gcactctttc gattattgcc cgcaggcaga tcatgcgcgg cactacccgg
      841 ctttggtcgt gttcgtattt ggcaatcaga gccggaaaat gaatggtgtt atcgagaccg
      901 acggtgaaaa cagaaagggc cagcaagccc agttcgaaag cagcacccca ggctaacagt
      961 atgaaacgtc gcccggccag ggccggaacc agaagaggaa tggcaaactg cggcttaaag
     1021 gaggccagag ccataccggc accggccagg gcatctttct tatgcaagag tgcgaagaaa
     1081 aagaggctca agaagaggta ttgatagaaa caggcgtggc cgatgaagag ggtaaaccat
     1141 gagggctggg ccattaaaat ggcggtaagg aagcccaggc aggcaagcgg cgtggcttcc
     1201 ttccagccgc gcagtagaag cagagagccg ccggccatac atattgcaga aagcaagatc
     1261 cagaccagga aggaggtgtc tactggtaac aatgcccagg gcgccatcag gacaatgaag
     1321 aagggaacgg ccggaatgat gcggtcttcc atgggaactg ccaccggcat ggctttgttt
     1381 atggcggccg ggtcatagat cctggtgcgg gtagaaggtt ctagaactag tttcccgcag
     1441 gtgtaaaact gtacaaagtc gctcaaatag agttgcttgt ttctggtgct gaaggccagt
     1501 tcattggtct gggcacgctt aacgagttca ccatagggca ccaccagcac ggcggcgata
     1561 atggtgaaat aggtgattgt gaggagtctg gctcgcaaca attccattag ttagtgaacc
     1621 atcccttgaa tgccgaagca aatgccgttt tgctgtggga ttttgcattg taccacctgt
     1681 ctattccgga ccggcttggc ttgcaccaga tgtggtgtgg tctcgggtat ttcggcccgg
     1741 gcaggtcttc ctatataaat gaatgttcgc cctccattta atggaataca ttaaaacgtt
     1801 ccggcggctc ctgagattta agcttgacca tgggagaacc ccccggtttc gtttacaaat
     1861 cgattacatg gattccggta taaatgttca tgacaatctc acgtagtcta tgtcaaatta
     1921 gccctgcgac cttaaaccag aacctaaggc agctgttttt ctaattgttc agaggtaata
     1981 ctcagatgcg agcaaatata ttagatgatc ggcccggagc agcaattttc tttccatttg
     2041 cggcaatagt cataccgata attctttcct tattccttac catcaccctg caaaaccccg
     2101 ccaacgccca atccactgtg tttgaagagc gccaccgggt ttcgggctgg gaagagcccc
     2161 tctgtgccag ggaaggcaat ctgcgccact actactggac cgaaatcgat gccagcagcc
     2221 gttatcgcgc caatgccata aaccgggttg ccccttccgg aagggcctcc tacaagatta
     2281 tcagcagaac cgccggacca aattctcgcc ct
//
LOCUS       DAOQNZ010000625         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28522_length_2311_cov_4.273050, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000625 DAOQNZ010000000
VERSION     DAOQNZ010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28522_length_2311_cov_4.273050"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PKW73_11390"
     CDS             complement(<1..1064)
                     /locus_tag="PKW73_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNA73937.1"
                     /translation="MVMTGAGTTSKASGKELNLKKRVYLFEEGFEAMGGDREAMKELL
                     GGKGAGLAEMTRSGVNVPPGLTILTSCCREYTAGGEKMPAGLFDEILSELAHVEKKLD
                     RKLGDLNKPLLLSVRSGAKFSMPGMMDTVLNLGLNDQTVESIATLTKNPRFAWDSYRR
                     FIQMFSNVVLEIGKDKFEDILDDMKDELKVSNDNELTVENLKKLVLEYKALVEKKKGK
                     PFPQDPKQQLHDSIEAVFRSWNNPRAVYYRNLNKIDHNLGTAVNVQSMVFGNFDDSSA
                     TGVCFTRNPSTGEKLLYGEYLINAQGEDVVAGVRTPLKISEMASDNQAIYDELLANVG
                     RLEQYYRDMQDIEFTIEQGKL"
     gene            complement(1076..1939)
                     /locus_tag="PKW73_11395"
     CDS             complement(1076..1939)
                     /locus_tag="PKW73_11395"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNA73938.1"
                     /translation="MSKLADGSAGEFRLPQGTNELIIFTMSDSSGETAESVARAALVQ
                     FEPGQASIYRLPQVRNSLQIPAMVKEISHGRAIIAYTLVLPEYRETLEAEANKFKIPT
                     IDLLSPLINRISHLTGASPLSQPGRLHLLDETYFRRIEAVDFAIRFDDGKNPDGILQA
                     DVVLIGVSRTSKTPNCMYLAHHYGLKAANVPLVYGVEPPLSLFQVNPRKIFGLHIDPY
                     LLQEIRSTRALVLGMPGSTDYADPDRIMQEVRHARKLFRELKCHIIDVSAKAIEETSS
                     EIFLLLREQTQ"
     gene            complement(2021..>2311)
                     /locus_tag="PKW73_11400"
     CDS             complement(2021..>2311)
                     /locus_tag="PKW73_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73939.1"
                     /translation="AALAQNRGLDVLGLLTVETQSGEGQVLENRYSRQDVEQVFKTRS
                     QAPYLGCIKYSPSINVQRVNQGNLIKMTESGVDLLALYKCMAEFGIDVAVAK"
BASE COUNT          548 a          626 c          590 g          547 t
ORIGIN      
        1 agcttgccct gttcgatggt gaactcgata tcttgcatat cgcggtaata ttgctccagg
       61 cgtccgacat tagccagaag ctcgtcgtaa atagcctggt tgtcggaggc catttcggaa
      121 atcttcaggg gtgtgcgcac accagccact acatcttcac cctgggcatt gatcaggtat
      181 tcaccataaa gcagcttttc gccggtgctg ggattgcgtg tgaaacaaac accagtggcc
      241 gagctgtcat cgaagttgcc gaaaaccatc gactgcacgt ttacagccgt accgaggttg
      301 tggtctatct tgttgaggtt gcgataatag acggcccggg gattgttcca ggagcgaaag
      361 accgcttcga tggaatcatg cagttgttgc ttgggatcct gggggaaggg cttgcccttc
      421 ttcttctcga ccagggcttt gtattccagc acaagcttct tcaaattctc aacggtaagt
      481 tcgttatcgt tggaaacttt cagctcatcc ttcatatcat cgagaatgtc ctcaaatttg
      541 tccttgccta tctccagaac cacattggaa aacatctgaa tgaagcgccg gtagctgtcc
      601 caggcaaagc gagggttctt ggtgagagtg gcaatcgatt ccacggtctg atcattgaga
      661 cccagattca agaccgtatc catcatgccc ggcatggaaa acttagcacc ggagcgcacc
      721 gaaagaagca agggtttgtt caaatcaccc agcttgcgat cgagtttctt ctcgacatga
      781 gccagctcgc tcaaaatttc atcgaagaga ccggcgggca tcttctcacc accggcggtg
      841 tactcccggc agcagcttgt gagaatggtc aaaccaggcg gcacattcac tccagaacga
      901 gtcatctcag ccagaccggc gcccttgccg cccagcagtt ccttcatcgc ctcgcgatcc
      961 ccgcccatgg cctcaaaacc ttcctcaaag agataaactc tctttttcag attgagttct
     1021 ttgccggagg ctttggaagt tgtacctgcg cctgtcatga ccatttttcg tacctctact
     1081 gtgtttgttc tctcagtaac agaaaaatct ccgaggatgt ctcctcaata gccttagcgg
     1141 acacatcgat gatgtggcac ttcagctcgc ggaaaagctt gcgcgcatgg cgcacttctt
     1201 gcatgatgcg atcgggatca gcgtagtcgg tggaaccggg catccccagc acaagggcac
     1261 gggtgctgcg aatctcttgc aaaagataag ggtcgatatg aagtccgaaa atcttgcggg
     1321 gattgacctg gaaaagtgaa agcggcggct ccaccccata aaccaggggt acattggccg
     1381 ctttcaaacc ataatgatga gccaggtaca tgcagttggg cgtcttgctg gtgcgggaca
     1441 caccaatcaa gaccacatca gcctgcaaaa tgccgtcggg attcttgccg tcatcaaatc
     1501 tgatggcaaa gtccacggct tcgatgcggc ggaaataggt ttcgtccagt aagtgcaggc
     1561 gaccaggctg tgacaggggg ctagcaccgg tcagatggga aattctattg atgagggggc
     1621 tcaagaggtc tatggtagga atcttgaact tgttggcctc ggcttccagg gtctccctgt
     1681 actcgggcaa aactaaagtg taagcaataa tggcgcgacc gtgcgagatt tccttgacca
     1741 tggccggtat ctgcagagaa tttcgcacct ggggcaggcg atatatggat gcctgccccg
     1801 gttcaaactg caccagagca gcccgcgcca ccgactccgc cgtctccccc gaagagtccg
     1861 acatagtaaa aatgatcagt tcattggtgc cctgaggcag cctgaactcg cccgcacttc
     1921 cgtccgccaa cttcgacata gccacccagg gaagaaattc tccagagcca acttttaaca
     1981 tatttgaagg gcaaagtggt ccagctacat atatgtttta ttacttggct actgccacgt
     2041 cgataccgaa ctcggccatg catttataga gcgccagtaa gtccactcca gactcagtca
     2101 ttttgatgag gttgccttga tttacccgtt gtacattaat ggacggcgag tacttaatac
     2161 agccaaggta aggggcctgg ctgcgcgtct tgaacacctg ctccacatcc tgacgactgt
     2221 agcggttctc cagaacctgt ccttccccgc tctgggtctc cacagtcaga agcccgagca
     2281 catcaagacc gcgattctga gccagggcag c
//
LOCUS       DAOQNZ010000626         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28524_length_2311_cov_3.871897, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000626 DAOQNZ010000000
VERSION     DAOQNZ010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28524_length_2311_cov_3.871897"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKW73_11405"
     CDS             <1..453
                     /locus_tag="PKW73_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA73940.1"
                     /translation="GYKADDIILYGESIGCGVTTGVMASRPCRAVILQSPFVSLMSTA
                     RDKLFFMRTLPDFVMVEPQLNNLAAVQKPHPPLLLMHGDKDKTLPCRYSEELFAKASE
                     PKQLFIVEGADHNDVYQDPGRGVLVTLKNFIASVNEAKSQVSLQTQAQ"
     gene            complement(428..1270)
                     /locus_tag="PKW73_11410"
     CDS             complement(428..1270)
                     /locus_tag="PKW73_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73941.1"
                     /translation="MAVQTQEITIAHSPDSDDAFMFYALAKDKLDTRGLTVHQVLKDI
                     QSLNQDAINGRYEVSAISFAAYPLVKERYKLMPCGASMGDNYGPMVIAKPGVNAENLK
                     GKTVAIPGKLTTAYLTMQLWQPGLTVVEVPFDQIIDYVVAGKADAGLIIHEGQLMFQE
                     SGLELIVDLGVWWQKETGLPLPLGGNVVRKDLGDKMVKEVTELFKSAIVFALEHRQDA
                     LDYAMTFARDMKRELADKFVGMYVNELTVDYGERGRKAVTKLFEMAFEKGITTELIVP
                     EFVD"
     gene            1475..2053
                     /locus_tag="PKW73_11415"
     CDS             1475..2053
                     /locus_tag="PKW73_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73942.1"
                     /translation="MKAALALVLGTSMVVLAPSALAGQLYPKVYDATYEAKSQNSVST
                     IRMCSDGKGKLRTESGTPAYKIISLIDYPGKFSYSILDSQKMIMKTPISTEYEGEMTP
                     ELAKKKKAQDLGTKDIAGHKCHGWKMPVEGGFTEVWSDEKAGITVHSVTEAKGYKSEM
                     TLKTYSPAPPAEALFALPQGYKVTSTGQPNSY"
     gene            2074..>2311
                     /locus_tag="PKW73_11420"
     CDS             2074..>2311
                     /locus_tag="PKW73_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73943.1"
                     /translation="MFDLKPLMESLQRVEALLQSIPGSLIIVNGETGLILGIDDRKAG
                     FVSQSNVKPAGEPLNLANIIKTERTPQELLTYMRG"
BASE COUNT          604 a          636 c          564 g          507 t
ORIGIN      
        1 ggctacaaag ccgacgatat tatcctctat ggagagtcca ttggttgtgg tgtcactacc
       61 ggtgtcatgg ccagccgtcc ctgcagagcc gtaatcttgc aatcaccctt tgtttccctg
      121 atgagcacgg ctcgggacaa gctcttcttc atgcgcaccc tgccggattt tgtcatggtc
      181 gagccccagt tgaacaatct ggcagccgtg caaaagccgc acccaccgct actcttaatg
      241 cacggcgaca aagacaagac cctgccctgc cgttattcgg aagaactctt tgctaaagcc
      301 agcgaaccca agcagctatt catagttgaa ggcgccgacc acaatgatgt ctaccaggac
      361 cccggtcgcg gtgtgctggt gacgcttaag aacttcatcg ccagtgtgaa cgaagcaaag
      421 tctcaggtta gtctacaaac tcaggcacaa taagttctgt ggtgatgcct ttctcgaaag
      481 ccatctcaaa cagcttggtg acagccttgc gtccgcgttc gccgtaatcg acagtcagtt
      541 cgttcacata cataccgacg aatttgtctg ccagttcacg cttcatgtcg cgggcgaaag
      601 tcatggcata atccagcgca tcctgcctgt gctcaagagc aaagacaatt gcgctcttga
      661 agagctcggt cacttccttg accattttgt cgcccaggtc tttgcgcacc acattgccgc
      721 cgaggggcag gggtagaccg gtttctttct gccaccagac accaagatcc acgatcagtt
      781 caagaccgct ttcctggaac atcaactgcc cttcatgaat aatcaaaccg gcatcggctt
      841 tgccggccac tacgtagtcg ataatctgat cgaaaggcac ttccaccacg gtgagtccgg
      901 gctgccagag ttgcatggtc aaataagccg tagtcaattt accgggaatg gccactgtct
      961 ttcccttgag attctcggca ttaacgcccg gcttggcaat caccatggga ccataattgt
     1021 cgcccatgct ggctccgcag ggcatgagct tgtagcgctc tttgacgaga ggataagcgg
     1081 caaaagagat ggccgaaact tcgtagcggc cgttgatggc atcctggttc aaagactgaa
     1141 tgtccttcaa tacctgatgc acggtcagac cacgcgtatc gagcttgtct ttggccagag
     1201 catagaacat gaaggcatcg tccgaatccg gagagtgggc gatagtaatt tcctgagttt
     1261 ggacagccac gtttctttcc cccgagtagt cagtactgca ttaacagtac taaatgagca
     1321 ccggctcgga ctccgtccaa taccgactgc tgctatcaat caagtcaaat tgtccaccga
     1381 aataggctaa atgagcatga gcaaattcaa agaaagggtg atatcctctt gccgggccgg
     1441 ccaagaaggc ggctattagc aggggtaaat gtttatgaaa gccgcattag ctttggtttt
     1501 gggaacctcc atggtagttc tggcaccctc agccctggcc gggcaattat atccaaaggt
     1561 ttacgatgcc acctacgaag ccaaaagcca gaattcagtc tccaccatcc gcatgtgctc
     1621 cgacggcaag ggcaaactgc gcactgaaag cggcacgccg gcctataaaa tcatctcttt
     1681 gatcgactac cccggcaaat tctcctattc catcctcgac tcccagaaaa tgatcatgaa
     1741 aacccccatc tccaccgaat acgagggaga gatgacccct gaactggcta agaaaaagaa
     1801 agcccaggac ctcggcacta aagacatcgc cgggcacaaa tgccacggct ggaaaatgcc
     1861 tgtggaaggt ggtttcaccg aagtgtggtc ggacgaaaaa gcaggcatca cggtgcattc
     1921 ggtgacggaa gccaaaggct ataagagcga gatgaccctc aagacctact ccccggcgcc
     1981 ccccgcagaa gcactcttcg ccttacccca gggctacaaa gtaaccagca cgggtcagcc
     2041 caatagttat taacggttaa atgggacagg cagatgtttg atctcaagcc tttgatggaa
     2101 tcacttcaaa gagttgaagc actcctgcag tcaattcccg gcagccttat catagttaac
     2161 ggagagactg gcctcatttt gggaatagac gaccggaagg caggattcgt atcccagagt
     2221 aacgtaaagc ctgctgggga gcctttgaat ctagcaaaca tcataaaaac ggaaagaacg
     2281 ccgcaagaac ttctaaccta catgcgtgga a
//
LOCUS       DAOQNZ010000627         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28672_length_2305_cov_3.440889, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000627 DAOQNZ010000000
VERSION     DAOQNZ010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28672_length_2305_cov_3.440889"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKW73_11425"
     CDS             <1..245
                     /locus_tag="PKW73_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73944.1"
                     /translation="ANPWLDARMSLQTEEQMGRRSLMLVAYFVMSLALSAVWMKWIRA
                     EAKAQKNVAKRTAIQPAGALKGHDNNQSDEPREGGQ"
     gene            251..742
                     /locus_tag="PKW73_11430"
     CDS             251..742
                     /locus_tag="PKW73_11430"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA73945.1"
                     /translation="MAKIILMVGIPGSGKTTLSQRVVSKGFHYLNADSIRLELYGDEK
                     EQGKPEDVFAIFFARLEEAMEQKKNIIIDNTNLNPRQRKPILERAQQFGYEDVQLWLL
                     DVPLELCLERNRKRERAVPDDIVSNMYTELYKNGRPKRVEGKVVVIRPSQDGSDYLFF
                     PQS"
     gene            813..2132
                     /locus_tag="PKW73_11435"
     CDS             813..2132
                     /locus_tag="PKW73_11435"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA73946.1"
                     /translation="MAAVRAKIMAGALLSLSLCSFGQTVLAKTEALESKPKAVDGAAR
                     TLTEADVLRSESLDLLMEHMMKTWRLPAGQISVGIKGKVLYNRSYGKSDKEQKSPTTT
                     ANLFRIASVSKVLTAAAIHKLIDEGKLSYEDHAFSILDNLTPPKVGDTESNKAVDPRL
                     KDVTIGQLLTHSGGWTIEHGDPQRIYARMAADACGEPRPASPTAIVRYMMNKPMDYTP
                     GSKSVYSNFGFNILGRVIEKVSGKPYDVYVKEAILKPLGIETMELGRTQLKNARLNEV
                     YYHAGGDPFGVWSLFDQDGEVTDFAYGGDAAIESMDSHGGWIACAQDLVKFGMALDGQ
                     GQVKILQPETLKKFLTPSQYSAGRGSTRRAGGVVVDAGKKPHYRWTHAGALAGTSSVL
                     YSLNDGKVVAMIFNHLPANLGGFFQELETRLLKEMNTKKWRENALPE"
BASE COUNT          630 a          631 c          607 g          437 t
ORIGIN      
        1 acgccaatcc ctggttggat gccagaatgt ctttgcaaac ggaagagcaa atggggcgcc
       61 gctccctcat gttggtggca tattttgtca tgagtttggc cctttcggcg gtctggatga
      121 agtggattcg agcggaagcc aaagcccaga aaaacgtcgc taagcgcact gccatccagc
      181 cggccggtgc actgaaaggc catgacaaca accagagtga tgagccccga gaaggagggc
      241 aataacaaac atggcaaaga tcattctcat ggtcggaata ccgggttccg gcaaaaccac
      301 actctctcag cgggttgtct ccaagggctt tcattatcta aacgcagatt ccatacgcct
      361 ggaactatac ggagatgaaa aagagcaggg caagccggag gacgtttttg ccatcttctt
      421 cgccaggctg gaagaagcca tggaacagaa gaaaaacatc atcatcgaca acaccaatct
      481 caacccccgg cagcgtaagc ccattctgga aagagcccaa cagttcggct acgaagacgt
      541 acaactgtgg ctactggatg tgccgctgga actttgcctg gagcgcaatc gcaaaagaga
      601 acgagccgtg cccgacgaca ttgtctccaa catgtatacc gaactctaca aaaacggccg
      661 gcccaaaaga gtggaaggca aagtggttgt aatcagacca tcccaggacg gcagcgatta
      721 tctcttcttt ccccagagct gatgcaacaa gatcgaaaaa cagacaagac taccaccgaa
      781 gaaccactga aaaaaaacgg agactactag aaatggcggc agtgagagca aaaataatgg
      841 caggggcact tttgagcctg tcgctgtgca gcttcggtca gacggtcctg gcaaagacgg
      901 aagccctgga aagcaagcca aaagcagtgg acggcgcggc gcgtactcta accgaagctg
      961 acgtactgcg ctccgaaagc cttgatctgc tcatggagca catgatgaag acctggcgcc
     1021 ttcccgccgg tcaaataagt gtgggcatca aaggcaaggt tctctacaac cgttcctacg
     1081 gtaagtcgga caaagagcag aagtccccca ccaccacagc caatctcttt cgcattgctt
     1141 cggtatcaaa agtcctgacg gctgccgcca tacataagct gatcgatgaa ggaaagttga
     1201 gttacgaaga tcatgctttt tccatactgg ataatctgac tccccctaaa gtgggcgaca
     1261 ccgaaagcaa caaagccgtt gaccctcgct tgaaagatgt gacgatcgga cagcttctca
     1321 cacacagcgg cggctggact atcgagcacg gcgatcccca gcgtatctat gcccgaatgg
     1381 ccgccgacgc ctgcggcgag ccccgcccgg ccagccccac ggccattgtg cgctatatga
     1441 tgaacaagcc aatggattat acgccgggca gcaaatccgt ctactccaac ttcggcttca
     1501 acatccttgg tcgcgtcatc gaaaaggtga gcggcaaacc ctacgacgtt tatgtcaagg
     1561 aagccatctt gaaaccgctg ggcattgaaa ccatggaact gggccgcacc cagcttaaga
     1621 acgctcgttt gaatgaagtt tattaccatg ccggcggcga ccctttcggc gtctggtctc
     1681 tgttcgatca agacggtgaa gtgacggact ttgcctacgg cggagacgcc gccatcgaga
     1741 gcatggactc ccacggcggc tggattgcct gcgcccagga cctggttaaa ttcggcatgg
     1801 ctcttgatgg tcagggtcag gtcaaaatat tgcagccgga aactctgaaa aagtttctta
     1861 cgccaagtca gtattccgcc ggccgcggca gcaccagacg ggccggggga gttgtggtag
     1921 atgccggcaa aaaaccgcac tacagatgga cccacgccgg agccctggcc ggcacaagtt
     1981 cggtcctcta ctcgctcaat gacggcaaag tagtggcaat gatcttcaat catttgccgg
     2041 ccaacctggg gggatttttt caagaactcg agaccagact cttgaaggaa atgaatacca
     2101 aaaaatggcg agaaaacgcc ctgcctgaat aggcgcttct gacgacgact tcaaaggagg
     2161 agaaggctgc accagctatg agacctagat ttctttgccg caaaccagtt ccaaacgaga
     2221 caagaaaatt gcgccatgcc gaaaacaggc tcaagggcct gacggcggca acgctaacat
     2281 tggctctcgc gctctctttc gcagg
//
LOCUS       DAOQNZ010000628         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28683_length_2304_cov_4.579369, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000628 DAOQNZ010000000
VERSION     DAOQNZ010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28683_length_2304_cov_4.579369"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2304
                     /locus_tag="PKW73_11440"
     CDS             <1..>2304
                     /locus_tag="PKW73_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73947.1"
                     /translation="LLAFATKSGTNLSTLVGSTATANFAAENMIASLRGTGYSAEGFS
                     QQTLNAAIAPFFQQIRQSHNLLANRPTETTQPTSHSEAESKPSTGHQVDYRSLADIQA
                     QNLAKQEAEFKQNKAWFDKETLPVKEQELQAARAQLTDTLVRDAGELIARSTGIPLEE
                     GESLARKIGIDLKDASENPGAQGQFDPRSGRIDLYVGDPDSSVKPGQTVNHETAHLLD
                     AARNQALFEANPKLFLETLVDDSMAGAFSKGEARLEKAVFGERTFEREALADKNGFTA
                     DDAKFAREQIKAYMTENMKDGKLPAVPSAEELNKFIYQRGADFPHAQFSHNQALIHEM
                     RREISNLRVTFNNTGLAPEALAREPVRSLVDLTRKTLGENPSREQYVNALAGASDAAL
                     ALGSQSNAYYDFGSRYENKANRLQYSRTLEGALGESQGQTRQLQRQLHELAADGQLEW
                     KLSSAQTARHLKELTTALDGAGTSTVKMLENLNNPAGKAALAALANEPVLPAALKETA
                     RQLSANMQVVEESTRALRFLTALDMVARDSAKLKMQDPAVSKEELEASRKQWLESAFA
                     NAPQGEEARLAAYMVRRGYVSQEELQFSSTKLKPSPDGKLSETSEQDRAEIKYSKLNE
                     TDAAGDQARAHQEIDKLAHKNQVDGLLEKKLIQKTADFLKGMVAEGLPLDGALNVIDL
                     VNVTKTENSYPNKTALKELLSLSAEQSNELLGLIPKKDLVQIMDALNLGLMNGKDLKA
                     ILADSRRLEATPGYQQCREYFADLDPG"
BASE COUNT          662 a          645 c          603 g          394 t
ORIGIN      
        1 cgctgcttgc cttcgccact aaaagcggta ccaatctctc caccctggtg ggcagcaccg
       61 ccaccgccaa tttcgcggca gaaaatatga ttgccagcct cagaggcacc ggctattcgg
      121 cagaaggttt ctcacagcaa acactgaacg cagccatcgc accgttcttt caacagataa
      181 ggcagtccca caacttactt gcaaacagac cgacagaaac tacgcagccc acctcccaca
      241 gcgaagccga gagcaaacca tccaccgggc accaggtgga ctaccgcagc ctggccgata
      301 tacaggctca aaacctggcc aaacaggagg ctgaatttaa gcagaacaaa gcctggttcg
      361 ataaggaaac cctgccggtg aaagagcagg agttgcaggc ggccagagcg cagctcaccg
      421 ataccctcgt cagggacgcc ggtgaactga ttgccagaag cactggtata ccactggaag
      481 aaggcgaaag tctggctcga aaaataggca tcgatctcaa agacgcctcc gaaaatcccg
      541 gcgcccaggg acagttcgac ccgcgcagcg ggcgcattga cctctatgtg ggagaccctg
      601 attcgtccgt caagcctggt caaaccgtta atcacgaaac cgcccacctt ctggatgccg
      661 ccaggaatca agcccttttt gaagccaatc ccaaactctt cctggaaacc ctggtggacg
      721 acagtatggc aggcgccttt agtaagggtg aagcaagact ggaaaaggct gtcttcgggg
      781 agcgtacttt tgaaagggaa gccctggcgg acaagaacgg attcactgcc gatgatgcta
      841 aatttgccag ggagcaaatc aaagcctata tgaccgaaaa catgaaggac ggcaaactgc
      901 cggcggtgcc tagcgcggag gaactgaaca agttcatcta ccagcgcggt gccgactttc
      961 cccacgcgca gttcagccac aatcaagccc taattcacga gatgcgccgg gaaatttcga
     1021 acctgcgcgt caccttcaac aacaccggtc tggctcctga agctttagcg agagaaccgg
     1081 tgcggagtct tgtggacctg acaagaaaaa ctcttggtga aaatccaagc cgagagcagt
     1141 atgtcaacgc cctggccggt gcctcagacg cggctctggc cctcggtagc cagtccaatg
     1201 cctattatga cttcggctcc cgctatgaaa acaaagccaa ccgcctgcaa tactcccgca
     1261 ccctcgaagg tgccctgggc gaaagccagg gtcagaccag gcaattgcag agacagttgc
     1321 acgaacttgc cgccgatggg caactggaat ggaaactcag ttcggcacag accgccaggc
     1381 acctcaaaga gctaaccacc gcccttgatg gggcaggtac gagcacggtc aagatgctgg
     1441 agaacctcaa caacccggca ggcaaagcgg ccctggcggc ccttgccaat gagcccgtcc
     1501 tgcctgcggc cttgaaagaa acagcaagac aactgagcgc caatatgcaa gtggtggaag
     1561 aatcaacaag ggcactgcgt tttcttactg cactggatat ggtggcacga gactcagcta
     1621 agttgaagat gcaagacccg gcagtttcta aagaagaact ggaagcaagc cgcaaacaat
     1681 ggctggaaag tgcctttgcc aacgcccccc agggtgaaga agcaagattg gctgcttaca
     1741 tggtacggcg tggttacgtc agtcaagaag agctacagtt ctcatcaact aagttgaaac
     1801 catccccaga tggcaaactg tctgaaacaa gcgagcaaga ccgagccgaa attaaatata
     1861 gcaaactaaa cgaaaccgac gcagccggcg accaagccag agcgcatcaa gaaatagata
     1921 aactggcaca caaaaaccag gtggatggac tgctggagaa aaagctgatc caaaaaaccg
     1981 cagatttcct gaaaggaatg gttgctgagg gactccctct cgacggagcc ctgaacgtca
     2041 tcgatctggt aaatgtgacc aagacggaaa acagttatcc aaacaagacc gcgctgaagg
     2101 agcttctctc gctttcagcg gaacagtcga atgaattgct cgggctcatt ccaaagaagg
     2161 acctagtgca gatcatggat gcactgaatc tagggctaat gaatggcaaa gacctgaagg
     2221 caattctggc ggattccaga agacttgagg ctaccccggg ctaccagcag tgccgcgaat
     2281 actttgccga cctagatcca ggaa
//
LOCUS       DAOQNZ010000629         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28744_length_2302_cov_3.846462, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000629 DAOQNZ010000000
VERSION     DAOQNZ010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28744_length_2302_cov_3.846462"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKW73_11445"
     CDS             complement(<1..549)
                     /locus_tag="PKW73_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73948.1"
                     /translation="MSERLLTKAKSSAKPNTKLTVLAFLVIASFSLLLFPQTAQAQVG
                     VPQPPPPGDGSAPPPLDPGDTGGAPAVPSGAADGSAGTNFPVGAGSDRTVVRIYKMDG
                     RGQRMVQTIRPDTLTPQQQAQIGGILGVNMNSGEQSVDVTVSDAQLEQLNEAMAAYPQ
                     TSMNGGRKQITGDNPAAGRPLPG"
     gene            complement(571..>2302)
                     /locus_tag="PKW73_11450"
     CDS             complement(571..>2302)
                     /locus_tag="PKW73_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73949.1"
                     /translation="LLQAIFGKVAEEMGDTSLRVPHGSGGRVIDVRIFTPETTPELKA
                     GIICEIEVLIARLCPLEVGDKLAGRHGNKGIVAIVVKDCDMPYLPDGTPVDLVLNPLG
                     VPSRMNVGQVFDTQLGLYASILNRYYRIHQFDESIAPDASFRLVTDALKEVRKLPNYQ
                     WMGEDGKVKLYDGRTGEAFDRPVLVGRQYMLKLNQLVLHKINARSGLGGPYAAVTQQP
                     VGGKANHGGQRMGEMEVWAIQAYGAANVLHEMMTIKADDIQGRHQSYADMVQGNEIQP
                     GGRTAAFDGLCCEIRGLGMEVTLGKVVDTFEPMPLKGVAEDGESAKEKKDDGTPTKTL
                     EEHYQVSRVDKLEAGDGTKILEIPAGDYVPVTKPPAPPVITSITSGKHGGKPRIEMSQ
                     EPLFDDIDESQVPKAEEKPASTPSFVDAFISEITLGGDEDEMDMPDDIPKDFDTAVQK
                     KAQPQSPQVTPPPNGDHGKANLPELPELLAREIGSNLVRPGGISGFPAFSELTVLARE
                     LAAITGKRQNESQSQAAQAELKTETAIESELPPSGYFKQPADGRRIIEKEAAHSPTEE
                     NKQPSDENSS"
BASE COUNT          397 a          634 c          675 g          596 t
ORIGIN      
        1 accgggcaga ggcctgcctg ctgccgggtt gtcgccggta atttgtttgc gtccgccgtt
       61 cattgaggtt tgcggatagg cggccatggc ttcgttgagc tgctctaatt gggcatcgga
      121 gacggtgacg tccaccgact gctcgccgga attcatattg actccgagaa tgccgcctat
      181 ctgagcttgc tgttgaggcg tcagggtgtc gggtctgatg gtttgcacca tgcgctgacc
      241 acgtccatcc attttataga ttcgtactac ggtgcgatcg gaaccggcac cgacgggaaa
      301 atttgtaccg gcactgccgt cggccgcgcc ggatggtact gccggtgcgc caccggtgtc
      361 gcccggatca aggggcggcg gtgcactgcc gtcaccgggc ggcggcggtt gcggtacgcc
      421 tacctgggct tgggctgtct ggggaaagag caggagggag aaactcgcga tcaccaggaa
      481 agccaacact gtcagtttcg tgttcggttt tgcgcttgat ttggcctttg taaggagtct
      541 ttcagacatc tgttccctct acttaactcc ctagctggag ttttcatcgg acggttgctt
      601 gttttcttcc gtgggtgagt gagccgcttc cttctctatt atgcgcctgc catccgccgg
      661 ttgtttgaag tagccgctgg gcggcagttc actttcaatt gcggtttcgg ttttcaactc
      721 cgcttgggct gcctgacttt ggctttcatt ctggcgcttg ccggttatgg ctgccaactc
      781 tctggccagg accgtaagtt cagagaaggc cgggaagccg cttatgccgc ccggtctaac
      841 caggttactg cctatctctc tggccagcag ttcgggcaat tccggtaagt ttgcttttcc
      901 atggtctccg tttggcggcg gtgtaacctg ggggctttgc ggctgcgctt ttttctgaac
      961 tgccgtgtcg aaatctttgg gaatgtcgtc cggcatatcc atttcatctt cgtcgccacc
     1021 gagggtgatt tcgctgatga aagcgtccac gaaggaaggt gtcgaggctg gtttttcttc
     1081 agcttttggc acctgactct cgtctatgtc atcaaacaac ggttcctgcg acatttcaat
     1141 acgtggtttc ccaccatgtt taccgctggt gatagaggta atgaccggcg gcgccggtgg
     1201 tttggtgacc ggaacgtagt caccggcggg gatttccagg attttggtgc cgtctccggc
     1261 ttccaacttg tcaactcggg agacctgata atgttcttcc agagttttcg taggagtccc
     1321 atcatctttc ttctcttttg cactttcgcc gtcctcggcc acgcctttga gtggcatggg
     1381 ttcgaaggtg tccaccactt tgccgagggt gacttccata ccaagaccgc gaatttcgca
     1441 gcaaagtccg tcaaaagcag cggtgcggcc gcccggctga atttcattgc cctgcaccat
     1501 atcggcataa gactggtgcc tgccctgtat gtcgtcggcc ttgatagtca tcatttcgtg
     1561 caatacattg gcagcgccgt aggcctggat ggcccagact tccatctcgc ccattctctg
     1621 accgccgtgg ttggctttcc cacccacggg ctgctgggtg acggcggcat agggaccacc
     1681 caagccggag cgggcgttaa ttttgtgcaa gactaactgg tttagcttga gcatgtactg
     1741 ccggcccacc agaaccggtc tgtcgaaagc ttcaccggtg cggccgtcgt agagtttgac
     1801 cttgccgtcc tcacccatcc attgatagtt gggtagtttg cgaacttcct tgagggcatc
     1861 ggtgaccagc ctgaaactgg catccggggc gatggattcg tcgaactggt ggatacggta
     1921 atagcgattg aggatgctgg catagagacc caattgggtg tcgaagacct ggcccacgtt
     1981 catacggctg ggcacgccca ggggattgag caccagatca acaggggtgc cgtccggcag
     2041 gtagggcatg tcgcagtctt tcaccacaat ggccacgatg cctttgttgc cgtggcgccc
     2101 ggccagtttg tcgcctactt ccaggggaca gagcctggca atcaagactt ctatttcgca
     2161 gatgatgcct gctttcaatt ccggtgtggt ttccggcgtg aagatgcgca catcgataac
     2221 gcgaccgccg gagccgtggg gcacgcgcaa gctggtgtcg cccatctcct cggccacctt
     2281 gccgaagatt gcttgcaata at
//
LOCUS       DAOQNZ010000630         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28896_length_2296_cov_4.101740, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000630 DAOQNZ010000000
VERSION     DAOQNZ010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28896_length_2296_cov_4.101740"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..669)
                     /locus_tag="PKW73_11455"
     CDS             complement(16..669)
                     /locus_tag="PKW73_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNA73950.1"
                     /translation="MITQPLGDAPEKTQEALLLYLKRQGELTVADLCGFLGITSMAVR
                     RHLVHLQKDGLVEARMVRQSRGRPTYRYKLSDKAEGLFPSASANLAEDILDAVLETAG
                     HKGVMDLLNLRNQKRLEKLGARIAGKNLKERVAEVSRIFSEDGYMTEWEELPDGNFLI
                     YQRHCAVHDLANKFRQVCAMEPSLIESLLGVKVTREKYILRQDPLCAYLVHTESQTL"
     gene            1046..1819
                     /gene="sufC"
                     /locus_tag="PKW73_11460"
     CDS             1046..1819
                     /gene="sufC"
                     /locus_tag="PKW73_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNA73951.1"
                     /translation="MTAQKPILEIVDLHVNVEDKEILKGVNLTIKAGEIHAIMGRNGS
                     GKSTLSYTVMGHPRYKVTSGKMLYKGEEISQMSPDERAKLGIALAFQYPVAIPGVSVS
                     NFLRKTTNACRGREIPVKEFRQELKALMAKLGVKDEFLSRYVNDGFSGGEKKRLEILQ
                     LSLLKPSLAVLDETDSGLDIDALKTVSEGVNAMVSPETAILLITHYQRMLDYVQPDFV
                     HVFQDGKIITSGGKELALELESRGYEWVQDTTVNQAVSV"
     gene            1838..>2296
                     /locus_tag="PKW73_11465"
     CDS             1838..>2296
                     /locus_tag="PKW73_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNA73952.1"
                     /translation="MSRAETKEDQATPDLSLNEIGNDYKYGFHDEEDYIFKSGRGLTR
                     EIVEKISAMKKEPQWMLDFRLKALDIFMKKPMPTWGNCQMLNDIDFQNIFYYIKPSMK
                     GEKNWEDVPDNIKNTFDRLGIPEAERKFLAGVTAQYESEVVYHSIREDLEK"
BASE COUNT          605 a          612 c          557 g          522 t
ORIGIN      
        1 aagggcatct aaaatctaaa gtgtttgcga ctctgtgtgc acaagatagg cgcagagcgg
       61 atcttggcgc aagatatact tttccctggt caccttcacg cctaaaagcg attcaatcaa
      121 actgggctcc atggcgcaga cctgcctgaa tttgttggcc agatcgtgca cggcgcaatg
      181 cctctggtag ataaggaaat tgccgtccgg caattcttcc cactcggtca tatagccgtc
      241 ttcggagaag atgcgggata cttccgccac tcgctccttc aaatttttgc cggcgatgcg
      301 agcgcctaat ttctcaagac gcttctggtt acgcaggttg agcaagtcca tcacaccttt
      361 gtgtccggct gtctccaaaa ccgcatccag aatatcttcc gccaaattgg cactggctga
      421 ggggaaaagc ccttcagcct tgtctgacaa tttgtatcga taggtaggac gcccccgcga
      481 ctgcctgacc atgcgggctt caaccagtcc gtctttctgc aaatgaacca gatggcggcg
      541 cacggccata gaggtaatgc ccaggaatcc acaaagatcg gccacggtca actcaccctg
      601 ccgcttcaga taaagcagca gtgcttcttg agttttttcc ggtgcgtcgc ccaggggctg
      661 tgtgatcatt tttagattcg gcttttgatt gagttatgtt gctgaaaatt ggcttttagg
      721 aaagctgagg cagtttttat tgaccgcaca gggcatcgaa aaattgccgg agagctagag
      781 attcggagtc caaataggag ggccacctgc ccgcccggga gcctcaagcc ccagaaattg
      841 ctatatccaa atacattata aagaaccggg gcaaacatat tctatttttg atactatttt
      901 atctataaaa tgctctgcgc cgaaggttgg aaagctttaa ggctcgttcc caagagtaga
      961 gcgtataaat agaatgcgta attatagata cctgtatctc ttatcttgag aagttattca
     1021 ccgccagttt aaggggaaac catagatgac cgcgcaaaag cccattttgg aaattgtcga
     1081 tctgcacgta aatgtggagg acaaggaaat tctgaaggga gtgaatctca ccatcaaagc
     1141 tggtgaaatc cacgccatca tgggtcgcaa cggttccggc aaatcgactc tctcgtacac
     1201 cgtcatgggg cacccccgct acaaagtgac cagcggcaaa atgctctata aaggtgaaga
     1261 gatttcccag atgtctcccg acgagagagc taagttgggt attgctcttg ctttccagta
     1321 tcccgtcgcc attcccggcg tctccgtttc caacttcctg cgcaaaacca ccaatgcctg
     1381 ccgcggcaga gaaatcccgg tgaaggaatt ccgccaggag ctgaaagcct tgatggctaa
     1441 actgggcgtc aaagacgagt ttctctcccg ctatgtcaac gacggcttct ccggcggcga
     1501 gaagaagcgc ctggagatct tgcagctttc cctgctcaag ccatccctgg ctgtgcttga
     1561 cgaaaccgac tccggtctag atatcgacgc cctcaaaacc gtttcagaag gcgtcaatgc
     1621 catggtctcc cccgagacag ccatcctcct tatcactcac tatcagcgca tgctcgacta
     1681 cgtgcagccc gactttgtgc atgtcttcca ggacggcaaa atcatcacct ccggcggcaa
     1741 agagctggcc ctcgaactgg aaagccgcgg ctacgaatgg gtacaagaca cgaccgtcaa
     1801 ccaggccgtt tcggtttagg aaaggcagga ggcatttatg tcacgcgcag aaactaaaga
     1861 agatcaggca acgcccgatc tcagcctgaa cgaaatcggc aacgattaca aatacggctt
     1921 ccatgacgaa gaagactaca tcttcaaatc cggtcgtggt ctgaccagag aaatcgtcga
     1981 gaaaatctcg gccatgaaaa aagagcccca gtggatgctg gatttccgcc tcaaggctct
     2041 cgatatcttc atgaagaagc cgatgcccac ctggggcaac tgccagatgc tgaacgatat
     2101 cgacttccag aacatcttct attacatcaa gccttccatg aaaggtgaga aaaactggga
     2161 agatgtgccg gacaacataa agaatacctt tgaccgcctc ggcattcccg aagccgaaag
     2221 aaaattcctg gccggtgtca ccgcccagta tgaatcggaa gtggtctacc actccattcg
     2281 tgaagacctg gaaaaa
//
LOCUS       DAOQNZ010000631         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28923_length_2295_cov_3.730357, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000631 DAOQNZ010000000
VERSION     DAOQNZ010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28923_length_2295_cov_3.730357"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKW73_11470"
     CDS             <1..1154
                     /locus_tag="PKW73_11470"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: protein motif:HMM:NF014612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HNA73953.1"
                     /translation="FVCYSGTAKPTIEDGLKAALKLGKKRIIVLPFLLFNGVLVKRIQ
                     DTAAAMQERQRPNAAGETTEILTAGYMGVHPHLAEVFAERAREGIMGKATMDCSLCKY
                     RVQIVGFERQVGEVQKSNHLRDPRTGELPPAKQLTPYQPHPIEAESMEIIAAGTQFQH
                     LSPLERSIAMRLVHTAGDFSAADDIFLSSGAAEAGVRALLRLRRLVTDVTMVESGLKR
                     SILKDLNISTWCGVHEEEAYLLAGSQGLTRSAAGIRLAFEKFGNDIIVAIGDAPTAIY
                     ELVRLVNEHYWRPQLVIGLPVGFVGTVDSKNKLRDCLQIPRITNAGTRGGSPWAATVL
                     NALLIEALARAANAQKDAAKVSAQESTFIKTGEKLETAHTNEPSLESLP"
     gene            1151..>2295
                     /gene="cbiD"
                     /locus_tag="PKW73_11475"
     CDS             1151..>2295
                     /gene="cbiD"
                     /locus_tag="PKW73_11475"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HNA73954.1"
                     /translation="MNTRVFDLAERAMNGLRRGRTTGSCAAAAVKAALGYLLTETVQE
                     EVNITLPEGNFYLPVPVQSIEALPDGSVKAEVVKMAGDDPDQTDGATIYAIVSLNKDK
                     DHPGVRFFAAEGVGTVTAPGIRVPVGEPAINPVPRRMICQVVEELLQQTTQANTCPPG
                     VDIKIGCVGGEEIAKRTFNPRLGIKGGISILGTTGIVEPMSLAAYQAAIEVYIRVALS
                     APGPANTVAYLPGNIGLKFARQVLQLNEKQTVHISNFVGYALAATEAYLEETDGELAT
                     LYLLGHPGKLAKLLDGVSDTHSQKSKMATSAVAKVAEQKAFSRDLVNICLEANTVEAI
                     IETTRDNPELSLNLWSSVQSSIETEVAKRKLKRIKNIAVRLFDMSGH"
BASE COUNT          600 a          680 c          606 g          409 t
ORIGIN      
        1 ccttcgtatg ctattccggc acagccaagc caacaataga ggacgggttg aaagcagccc
       61 tcaaactcgg caaaaaacgt attattgttt tgcccttcct tctcttcaac ggtgtcttag
      121 tcaaacgcat acaagacacg gccgcggcca tgcaagaaag gcagaggcca aatgcggccg
      181 gcgaaacaac ggaaattcta acagccggat atatgggcgt gcatccccat cttgccgaag
      241 tcttcgccga aagagccagg gaaggcatca tgggcaaggc cacgatggac tgctctttgt
      301 gcaagtaccg ggtgcagata gttggcttcg aaagacaggt gggggaagta caaaagtcca
      361 atcacttgcg agacccccgc accggagagt tgccgccggc aaagcagcta acaccctacc
      421 agcctcaccc gatcgaagca gagagcatgg agatcattgc cgccggtacc cagttccagc
      481 acttgagccc actggagcgc agcattgcca tgcgactggt gcatacggcc ggcgacttca
      541 gtgccgcaga cgacatcttc ctttccagcg gagcggcaga ggccggcgtg cgcgccctcc
      601 tgagactgcg caggcttgtc accgacgtta ccatggtcga aagcggttta aagcgcagta
      661 tcttgaaaga cctgaacatc agtacctggt gcggcgtaca cgaagaagaa gcctacctgc
      721 ttgccggcag tcagggcttg acccgctcag ccgccggcat acgcctggca ttcgaaaaat
      781 tcggaaacga catcattgta gccatcggtg atgcccccac ggccatttac gaactggtgc
      841 gtctggtcaa tgaacattac tggcgcccgc agctagtcat aggactgccg gtgggctttg
      901 tcggcaccgt agacagcaaa aacaagctga gagactgcct gcaaataccg cgcatcacca
      961 acgccggcac cagaggcggt tcaccctggg cagccacggt gctgaatgcc ctcctgatcg
     1021 aagccctggc cagagccgct aatgcccaga aggatgccgc taaagtttcc gcacaagaat
     1081 ccaccttcat taagacaggc gagaagctgg aaacggcaca cacaaacgag ccctctctgg
     1141 agtcgctccc gtgaacacca gagtctttga tctagcggaa agagccatga acggtttgcg
     1201 tcgaggacgc accaccggct cctgcgccgc cgccgccgtc aaagccgccc tcggctacct
     1261 gctcacagaa acggtacagg aggaagtcaa cattacctta ccggaaggaa acttctatct
     1321 gccagtgccg gtacagtcaa tagaagcact accggacggc tccgtcaaag cggaagtggt
     1381 gaaaatggcc ggagatgatc ccgatcaaac ggacggcgcc acaatctatg ccatagtcag
     1441 cctcaacaaa gacaaggatc atccgggtgt gcgcttcttt gccgccgagg gagtcggtac
     1501 cgtcaccgct cccggtatca gagtgccggt cggagaaccg gccataaacc cggtgccgcg
     1561 ccggatgatc tgccaggtag tagaagaact ccttcagcaa acaacgcagg ccaatacttg
     1621 ccctccgggc gttgatataa aaataggttg cgtcggcggg gaagaaatag caaagcgcac
     1681 attcaatccg cggctgggta tcaaaggcgg catttccatc ctgggaacca caggtatagt
     1741 cgagcccatg tctctggccg cctaccaagc agccatagaa gtctatattc gcgttgctct
     1801 ctcagcgccc ggaccagcca ataccgtggc ctacctgccc ggcaatatcg gtcttaaatt
     1861 cgctcgccag gtcctgcaac tgaacgagaa gcaaactgtc catatttcca atttcgtcgg
     1921 ctatgctctc gctgccactg aagcctatct agaggagacc gacggcgagt tggcaacact
     1981 ctacctgctc ggtcatccgg gcaagctggc caaactactg gacggagtct cagacaccca
     2041 ctcacaaaaa agcaaaatgg ctaccagcgc cgtggcaaaa gtagcagagc aaaaggcttt
     2101 ctcccgagat ctggtaaaca tctgcctgga ggccaatacg gtggaagcca tcatcgaaac
     2161 cacaagagac aacccggaat tgagcctcaa cctctggagc tccgttcagt cgtccatcga
     2221 aacggaagtg gcgaaacgaa agctaaaacg cataaaaaac atagcagtac gcctcttcga
     2281 catgagcggc catca
//
LOCUS       DAOQNZ010000632         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_28957_length_2294_cov_3.832068, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000632 DAOQNZ010000000
VERSION     DAOQNZ010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28957_length_2294_cov_3.832068"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..2102)
                     /locus_tag="PKW73_11480"
     CDS             complement(252..2102)
                     /locus_tag="PKW73_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNA73955.1"
                     /translation="METSKTIVDAFWKQVREKADKPAILHKVKGEYNSVIWREHGLIV
                     DLAMAGLAKNGIEQGSHVAILSQPNPRWTWSDLAALSLKAVTVPIYPTLAVPEVEYLI
                     KHSDCRCIMVEDEAQLHKVLQISTLPEKLKFVVLMKGEAPPSRPDLRIMTWDDLLKDG
                     EIYQLAHKEFLETLKNSVGPEDLASIVYTSGTTGVPKGVMISHGNIISVCRAIGDRLE
                     FKEQDLVLSFLPLSHVYERVGGQFLCIYFGITMAYAEAIETVPRNMVETHPTIINGVP
                     RFYEKAYQRIQFESKKLPKPQQILIRWALSLGRKYQEDEKVLTTKPSLVKQLHQAELR
                     AADRLVFSKIRRRFGGRLRMLVSGAAPLPKEVQDFFETIGLTIVEGYGLTETCAPVAC
                     NTPAQNRPGTVGKPLMGVEVKIAADGELLVKGGSVFSGYYKNQEATAEAFEDGWFKTG
                     DIAEIDPDGFIKIKDRKKDIIITAGGKNVAPQFIENLFKGDPLISHCLVYGDRRKFIT
                     CLFTLHKDGLKAFAEKNKLNFKEIEELTEHPLVRQEIERTVNQKNEGLANFERIKRFA
                     LLDRDFTIEDNELTPTFKVKRKLVTEKYRQVLDALYPEEDLEVENGLQKR"
     gene            complement(2113..>2294)
                     /gene="cutA"
                     /locus_tag="PKW73_11485"
     CDS             complement(2113..>2294)
                     /gene="cutA"
                     /locus_tag="PKW73_11485"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HNA73956.1"
                     /translation="EVVKDEESLLVMKTSTDFYARLEARVKALHSYDVPEVLALPVEK
                     GSKEYIGWLLGSLEK"
BASE COUNT          486 a          583 c          613 g          612 t
ORIGIN      
        1 ctgaatacgg tcatgagacc cggaatcagc aggcgcttgt cttgccagcc cattttgatg
       61 gaggcaagaa ttagtttcag accccttcca aatgattcaa acatgttcgg tatgacctca
      121 gaggcacatc aattgattta gtaaggtttt cgacccagct tgcgcatgag tcttcgcttc
      181 aaaaaaggtt tccagaacct ggaacctcgc cattattgta attagttgcc aagcgaatgt
      241 aaagaacttt tttatcgttt ttgcagccca ttttccactt ccaggtcctc ttcgggataa
      301 agagcgtcta atacctgccg gtatttttcc gttacaagct tgcgcttaac cttgaaagtg
      361 ggcgtcagtt cattgtcttc gatggtgaag tcgcgatcga gcagggcaaa acgcttgatt
      421 ctctcgaagt tagccagccc ttcgttcttt tgattgaccg tacgttcaat ctcttgcctg
      481 accaggggat gttcggtcaa ttcctcaatt tccttgaaat ttagcttgtt tttctcggcg
      541 aaagccttga ggccgtcttt gtggagggtg aaaagacagg ttatgaattt gcgccgatcg
      601 ccgtaaacca ggcagtggct tatgagcgga tcgcctttga agaggttttc gataaattgc
      661 ggggccacgt tcttgccgcc ggcggtgatg atgatgtctt tcttgcggtc tttgatctta
      721 ataaagccgt ccgggtcgat ttcggcaata tcgccggtct tgaaccagcc gtcttcaaag
      781 gcctcagcgg tggcttcctg gtttttgtaa tagccggaaa agacggaacc gcccttcacc
      841 agtagctctc cgtcggcagc aatctttact tccacaccca tgaggggctt gcctactgta
      901 ccgggtcggt tctgggctgg agtattacag gcaacggggg cacaggtttc ggtcagtccg
      961 tagccttcca cgatggtcaa gccgatggtc tcgaagaagt cctgcacctc tttgggcaga
     1021 ggagcggcgc cggaaaccag catgcgcagg cgccctccga aacggcgcct gattttggag
     1081 aaaaccaggc ggtctgccgc ccgtagttcg gcctggtgca gctgcttgac caggctgggc
     1141 ttggtggtca gcactttctc atcttcctgg tatttgcggc ccagggaaag ggcccagcga
     1201 atcagtattt gctgcggctt gggcagtttc ttcgattcaa attgaatgcg ctgataggct
     1261 ttctcgtaaa agcggggtac gccgttgatg atggtggggt gcgtctccac catgttgcgg
     1321 ggcactgttt caatggcttc cgcataggcc atggtgatgc cgaaataaat gcagagaaac
     1381 tgaccgccca ccctttcgta gacatgggag aggggcagga aagaaagtac caggtcttgt
     1441 tctttgaatt ccagacgatc gcctatggct cggcagacgc tgatgatatt gccgtgggaa
     1501 atcattaccc ctttgggcac gccggtggtg cccgaggtgt agacgatgga agccagatct
     1561 tccggaccga ctgagttctt cagtgtttcg agaaattctt tgtgggccag ctggtagatt
     1621 tcgccgtctt tcaacaaatc gtcccaggtc ataatacgaa ggtctggacg ggaaggcggc
     1681 gcttctccct tcatgagcac cacgaatttg agtttttccg gcagggtgga gatctgcaaa
     1741 accttgtgca gttgtgcctc gtcctcgacc atgatgcagc gacaatcgct gtgcttgatc
     1801 aagtactcta cttccggcac cgccagtgtc ggataaatcg ggaccgttac tgccttcaat
     1861 gaaagagccg cgaggtcgga ccaggtccag cgagggttgg gctgtgaaag gatggccaca
     1921 tggctgccct gctcaatgcc gtttttagcc agaccggcca tggccagatc cacgataagt
     1981 ccgtgctctc gccagattac cgagttgtat tcacctttca ccttgtgcaa aatcgccggc
     2041 ttgtccgctt tttctcggac ttgcttccag aaggcatcga cgatggtttt gcttgtttcc
     2101 atggttgcgg gattacttct caagacttcc taagagccag ccgatatatt ccttcgagcc
     2161 tttttccacc ggcagggcaa gaacctcagg cacgtcataa gagtgaagtg cttttaccct
     2221 cgcttcgagt ctagcgtaaa aatcggtcga ggttttcatg acaagcaggc tttcttcatc
     2281 cttgaccacc tcac
//
LOCUS       DAOQNZ010000633         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29031_length_2291_cov_4.270572, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000633 DAOQNZ010000000
VERSION     DAOQNZ010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29031_length_2291_cov_4.270572"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PKW73_11490"
     CDS             complement(<1..938)
                     /locus_tag="PKW73_11490"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HNA73957.1"
                     /translation="MSQTRILFQELVATCVLGTRSRSFNLPQPRTTDGATINALRQLE
                     RAAALGAETEGKKIAATQEELMLDQLALISTALRALGSQPSQAEEERLSQVNLEPCRP
                     EHLPEAPAAALSMLNQLIDDLSPVGQIFLARWFDLCREGGYRLSRHSIPRLLEKLKDK
                     EALREKLLTCGGEALPWLVKVNPDWHMYFAPRKAEAAELIKLFEEGSPTERLESLKLL
                     RSLDANLARETLAASWASETVESRVTFLKALEPFLSITDEEFLSGVALKDKRKEVREL
                     ACDFLTRICGSALQKRLQDFLGEQVQLKGGKLEITLA"
     gene            complement(935..>2291)
                     /locus_tag="PKW73_11495"
     CDS             complement(935..>2291)
                     /locus_tag="PKW73_11495"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNA73958.1"
                     /translation="SVKSGRELAVEKKWQNLGASDRALWGECQGSGAKPYQTRVDLSG
                     PAFKCSCPSRKFPCKHGLALFLLALQKNEIFAKTEAPGWVSEWLASRSEKSEKKKERE
                     EAPRSEEEQEKLLQAKEKRRQDRLDKVEAGLDELNLFLQDLVRQGTANLKSKPNSFWQ
                     ERAARLVDAQAPGLARRLNEAAGYTTTRAASPNSDPWQENLLLNLGQLRLIAQAYKNK
                     AQLNSSLQEDIASALGFTHSQEELLKEDGLKDTFLVLGQYVTQEDRLRVQRTYLLGKE
                     SGKLALILAFAHGVQPFERLLVPGKSFTGEIVYFPSNTPLRALIKAQGEALSEIFQPA
                     EGATAGSTRQKWSQWLSLCPFHQNMPVIFTEALLFQHEGRWWLRDQDGTHTEVTGNQA
                     DLWMVLAFSTGKALTLFGLFDGHTLSPLAALTNEGQYLKLSRSENKTDKEASLNRGVQ
                     V"
BASE COUNT          435 a          640 c          605 g          611 t
ORIGIN      
        1 gccagagtga tctccagctt gcctcccttt agttgaacct gctccccgag aaagtcttgc
       61 aggcgctttt gcagcgctga gccgcagatt cgagtgagga aatcgcaagc cagttctcgc
      121 acttctttgc gcttgtcttt gagggcgact ccactcaaaa attcttcatc ggttatgctc
      181 aggaatggct ccagagcttt caggaaggtg accctgctct ctactgtttc actggcccat
      241 gaagcagcca gagtctctct ggctagattt gcatcaagac tgcgcaagag ttttagactt
      301 tccagccgtt cggtgggaga cccttcttca aacagtttta tcagctcggc cgcttccgct
      361 ttcctggggg caaaatacat gtgccaatca gggttgacct ttaccagcca gggaagagct
      421 tcgccgccgc aggtcagtaa tttttccctc agggcttctt tatctttgag tttctcaagc
      481 agtctgggaa tgctatgccg actgagacgg tagccacctt ctctgcaaag atcaaaccag
      541 cgggctagaa agatctgccc caccgggctc aagtcgtcta tcaactgatt cagcatcgat
      601 agggccgccg ccggtgcctc tggtaagtgc tccggtcggc agggctcaag gttcacctgg
      661 ctcagcctct cttcttccgc ttggctgggc tgactgccga gggctcgcag tgccgtgctt
      721 atcagggcta attggtccaa cattaactct tcctgggtcg ctgcgatttt cttaccttcc
      781 gtttctgctc caagggcggc tgctctttcc aattgccgca gggcatttat tgttgcaccg
      841 tcagtagtgc ggggttgggg taagttgaag gaccggctcc tggtgccgag cacacaggtt
      901 gccaccaact cctgaaagag aattcgggtt tgactcatac ctgcacccca cgattcaggc
      961 ttgcttcctt atctgttttg ttttcggatc tggaaagctt cagatactga ccttcattag
     1021 tgagtgccgc caggggtgag agcgtatgac cgtcaaagag tccgaagagc gtcagagctt
     1081 tgccggtact gaaagctagc accatccata aatccgcctg gttgccggtt acttccgtat
     1141 gggtgccgtc ttgatcgcgc aaccaccagc gcccttcgtg ctggaagagg agagcctctg
     1201 taaaaattac cggcatgttc tggtgaaagg ggcaaagact gagccactga ctccattttt
     1261 gcctggtact accagcagta gcaccttccg ccggctgaaa aatttcggac agggcttccc
     1321 cctgcgcttt gatcagcgct ctcagagggg tgttggaggg gaaatagact atttctcctg
     1381 tgaagctttt gcccggcaca agcagtcttt cgaaaggttg aacgccgtgg gcgaaggcta
     1441 gaatcagcgc taatttaccg ctttccttgc ccagcagata ggtcctttgc acccgcagtc
     1501 ggtcttcctg tgtgacatac tggccaagca ccagaaatgt atccttgagc ccatcttctt
     1561 tgagcagttc ttcctgactg tgagtgaagc caagcgcgga agcaatgtcc tcctgcaagg
     1621 aagaattcag ctgcgctttg ttcttgtagg cctgggcgat caagcgcagt tgccccaggt
     1681 taaggaggag attttcctgc caggggtcgg agtttggcga ggccgctctg gttgttgtgt
     1741 agcctgcggc ttcattcaaa cgcctggcca gtcccggcgc ctgcgcgtct accaggcggg
     1801 ccgctctttc ttgccagaaa ctgttgggtt tgctcttcaa gtttgccgtg ccctgccgca
     1861 ccagatcttg gagaaagaga ttgagttcat caagaccggc ctcaaccttg tccaggcggt
     1921 cctgccttct cttctctttg gcttgcagaa gtttttcctg ttcttcctct gagcgcggtg
     1981 cctcctctcg ttctttcttc ttttccgact tttccgagcg ggaagcaagc cattcgctca
     2041 cccagcccgg agcttcggtt ttggcgaaga tctcattttt ttgcagtgcc agaaggaaga
     2101 gggccagtcc gtgcttgcaa gggaatttcc ggctcgggca actacatttg aaggccggtc
     2161 cgctcaggtc tactctggtc tggtaaggtt tggcgccgct gccctgacat tccccccaga
     2221 gcgctctatc gcttgctccc agattttgcc attttttctc aacggcaagt tccctgccgc
     2281 ttttgacaga g
//
LOCUS       DAOQNZ010000634         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29036_length_2291_cov_3.372540, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000634 DAOQNZ010000000
VERSION     DAOQNZ010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29036_length_2291_cov_3.372540"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PKW73_11500"
     CDS             complement(<1..737)
                     /locus_tag="PKW73_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73959.1"
                     /translation="MIKLRLLLVLAALTGLLSWLAYGTSLKVNLISEDWIHVYLAQEI
                     SDGQIQLLIDSFTRPWLQTSRVGIFYRPLIDLSFLFDVILRRLVNSFSSEPGEAAFIF
                     RLSNLNFHIITSFCTGLITYCLVKLSEGRQATRSALFAGALFAVCPLAQETLLWITCR
                     CDGLNTCLSSLCLTFYLLYRTRGSRLFRAAALLSYLLALGAKETSLAMPLVLLSLEIL
                     LMQDKRLILRLKEIAPFALVTIIFLAAR"
     gene            complement(734..1888)
                     /locus_tag="PKW73_11505"
     CDS             complement(734..1888)
                     /locus_tag="PKW73_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73960.1"
                     /translation="MKIQKNINLAGKLGLILVAGILLANAAQARGGGGGGRGGGMGGG
                     GRDFGGMGGVGRDFGGRDFGGIGRGFDGDRGLGDGRFGDDGFRRDDGPLSRVSEDALR
                     SGADRLWSPGDLAGRGLASDGLAFGARDSAITRNLSPAYLTGRGADLRRDFNRYDAFR
                     NNFWNRYPNAWWYRGWGDWYPWGAAGWSDLAGYWDVPVVDTPTDYDYGDNIYYNGDNV
                     YYGSQPVATTENYYYQAQSLATAPPPVSFKNGQPTNTGPASSWKPLGVYALSQNGEPS
                     TTLFQLAVNKDGTIRGNYYNSLTEDEKPIKGKVDKKTMRAAWMVEGNSTIIYEAGLSN
                     LLHPQCTALVHKGKSNTEQWLMVKMDKSMASAKPSPVSLNKSNLPSTRAL"
     gene            complement(1885..>2291)
                     /locus_tag="PKW73_11510"
     CDS             complement(1885..>2291)
                     /locus_tag="PKW73_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73961.1"
                     /translation="RGQGANAAIAMKLKVEEKPANFLCLTYMDKDGQIQATETIGVNP
                     KNKLISSWSFHKSGGVGTGYFVNEDNTWTKVAESYEADGLKGGAVYILKRLNGNAFTL
                     NSSSRYLAGSRLPNGNELLLEKDGANGGETGK"
BASE COUNT          504 a          609 c          643 g          535 t
ORIGIN      
        1 cgtgccgcca gaaagatgat agttactaag gcgaagggtg ctatttcttt cagtctgaga
       61 atcagtctct tatcttgcat tagcaagatt tccagggaga gcaaaaccag tggcatagcc
      121 agactggttt ccttggcacc gagagcaaga agatacgaga gcagagcagc cgctctaaac
      181 aagcggctgc ccctggttcg gtagagtaaa tagaaggtga gacagaggct gctcagacaa
      241 gtattgagcc cgtcgcagcg gcatgtgatc cagagcaggg tctcctgggc cagggggcag
      301 acggcaaaga gagcgccggc aaataaggcc gatcgagttg cctgcctgcc ttcgcttagc
      361 ttgaccaggc aataagtgat gagtcctgtg caaaagctgg tgatgatatg aaaattgaga
      421 ttgctgagcc ggaaaatgaa ggctgcttcc cccggttcag aggaaaaact gttgactagc
      481 cggcgaagta ttacatcgaa aagaaaggaa agatcgatga gcggacgata gaagataccg
      541 accctggaag tttgcagcca gggtcgcgtg aagctgtcga ttaatagttg gatttgacca
      601 tcgctgattt cttgagccag atagacgtga atccagtctt cgctaatcag attaactttg
      661 aggcttgttc cataggcaag ccaggaaagc agaccggtca gagctgctaa aaccaggagc
      721 agtctgagct taatcaaaga gctctggtag atggcaggtt ggatttgttg agactgaccg
      781 gacttggctt ggcagaggcc atgctcttgt ccatttttac catcaaccac tgttcggtat
      841 tgctcttgcc tttatgcacc agggccgtgc attgagggtg caataaattg gacagtccgg
      901 cttcatagat aatggtggaa ttgccctcca ccatccaggc ggcgcgcatg gtcttcttat
      961 ctaccttgcc cttgatcggc ttttcgtctt cggtcaagct attgtaatag ttgccgcgga
     1021 tggttccgtc tttatttacc gccagttgaa aaagcgtcgt ggatggctct ccattctggc
     1081 tgagggcgta cactcccagt ggtttccagc tggaagcggg acctgtattg gtaggctgcc
     1141 cgttcttgaa tgataccggc ggcggtgcgg tagccaggct ctgggcttga taatagtagt
     1201 tttcagttgt ggctacaggc tgagagccgt agtagacatt gtcgccgttg taatagatgt
     1261 tgtcgccgta gtcgtagtct gtgggcgtat ctacaaccgg tacgtcccag tagccggcca
     1321 ggtcggacca accggctgcg ccccaggggt accagtcgcc ccagccgcgg taccaccagg
     1381 cgttggggta gcggttccag aagttgttgc ggaaggcgtc atatcggttg aagtctctgc
     1441 gtaagtcggc gccccggccg gtcaggtagg ccggagatag attgcgtgtg atagccgaat
     1501 ctctggcccc aaaagccaga ccgtcactgg ccagaccgcg gccggccaga tctcccgggc
     1561 tccacaaacg atcggcgccg cttcgcagag catcttccga tacccggctt aagggcccgt
     1621 cgtctctgcg gaagccgtca tcgccgaagc gcccgtctcc taaaccgcgg tcaccgtcaa
     1681 aaccgcgacc gataccaccg aagtcgcgac cgccgaaatc gcgaccgact ccacccatgc
     1741 cgccaaaatc gcgaccacca ccacccatgc cgccgcctcg tccgccgccg ccgccgcctc
     1801 gagcctgggc cgcgtttgcc agcaggatgc cggccaccag gatcagaccc agtttaccgg
     1861 cgaggttgat gtttttctgt attttcattt gcctgtttcc ccgccgttgg cgccgtcttt
     1921 ctcaagcaga agctcattgc cgtttggcaa gcgagagcct gccagatacc gcgatgaaga
     1981 gttcaaggtg aaagcattgc cgttgagcct tttgagaata tagacggcgc ctccttttag
     2041 accatcagct tcatagcttt cagctacttt ggtccaggta ttgtcctcat tcacaaagta
     2101 gcccgtgccg acgccgccgc ttttatgaaa cgaccaggag gatatgagct tattcttcgg
     2161 attgacgccg attgtctcgg tagcttgaat ctgaccgtct ttgtccatat aggtcaggca
     2221 aaggaagttg gctggtttct cttccacttt cagcttcatg gctatagcgg cattggctcc
     2281 ctgtcctcgt g
//
LOCUS       DAOQNZ010000635         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29076_length_2290_cov_3.029083, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000635 DAOQNZ010000000
VERSION     DAOQNZ010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29076_length_2290_cov_3.029083"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKW73_11515"
     CDS             complement(<1..693)
                     /locus_tag="PKW73_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73962.1"
                     /translation="MTTKLNSYLLGELTINLGILTQEQMDKVLAISSDTGMPLGRVLV
                     LSGLILESDLTNLIRCQTLLRESMIEFEHASQVFQFILGTNRNLDDALMQFGWDGKQG
                     RKEALLGDFLVECDILTASQLEGYLRQQQRVKLPLGRMLVAAGVLTQEVLDKVLTVQA
                     MIRQGRFQRNEARDLILDARNRQPQAAAPKSKNFYAQPSKNRPKIGELLVIAGLISES
                     RLMEVLELSISER"
     gene            complement(906..1676)
                     /locus_tag="PKW73_11520"
     CDS             complement(906..1676)
                     /locus_tag="PKW73_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73963.1"
                     /translation="MDHSQKPDDLPKQLVEDVASGGDGSAFKEQISSMSNAERLKLVQ
                     DLNSSVLDANIANPGNRIILDVRLDNKDGNVKDIDLVRQAPPNSDGQKLNRIDLYDSG
                     DAAGKDADEKQRLDAVTAPYEQLYYTTIANMTRQEDLIGNYGSQQERESFKNMTLGNT
                     VDRMLQDVKDPEEREKLRANMLHNIDKIPDGQTTALTRPMDDLDSLASRHEKEPPRDV
                     QQRIEANIKLGSQLDRELSRRAPYLFPSDDALWPTEEE"
     gene            1979..>2290
                     /locus_tag="PKW73_11525"
     CDS             1979..>2290
                     /locus_tag="PKW73_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNA73964.1"
                     /translation="MFEAVLFDFDGTLVPSLDYWLSGFKHAFAELGHEVSEQEIIEGC
                     FYKDDSLVAEAFGIESHKTFWQLVQHKIVMHYDSAALVPGAREVLDFCRDRNLPLALV
                     TS"
BASE COUNT          439 a          582 c          642 g          627 t
ORIGIN      
        1 acgctcgctg atactcaatt ccagcacttc catgaggcgg ctttcggaaa tgagcccggc
       61 aattaccaac aactcaccga tcttgggtcg attcttggaa ggttgcgcgt aaaagttctt
      121 ggattttgga gccgctgcct gcggctgtcg gtttcgtgca tctagaatca ggtctctggc
      181 ttcgtttctc tggaagcgcc cctgcctgat catggcttgt acggtgagaa ctttgtccaa
      241 tacttcctgg gtgagtaccc cggcggccac cagcatgcgc cccagaggta gctttactcg
      301 ctgctgctgt ctgaggtagc cttccagctg gctggctgtc aagatgtcgc attccaccag
      361 gaagtcaccc agtagtgctt ctttgcgacc ctgcttgccg tcccagccga actgcatgag
      421 cgcatcgtca aggttcctat tggtgccgag aatgaactgg aagacctggc tggcgtgctc
      481 gaattcaatc atcgattctc gcaataaggt ctggcaacga atcagattgg tcaggtcgga
      541 ttccaagatc agacctgaaa ggaccagtac tcgtcccagg ggcatgccgg tgtcactgga
      601 aatggccagt actttgtcca tctgttcctg ggtgagaatt cccagattga tagtcagctc
      661 acccagaagg taagaattta gtttggttgt caccgaacgc tgctccgcgg ctccttgtaa
      721 attccggtgc cctgtgattt ccacgtgtaa aacggagcaa ctcggccctc aaacttgtgc
      781 caagtcgaga gaggcgcccg aaggagacgc ccatagcagt ctactatgtc tcgtatattc
      841 ttttgtagtc cttcataaaa ttttggcaaa agcggaccgg ctttttagac acggaagcct
      901 ggctactatt cctcttccgt cggccataat gcatcatccg acgggaaaag ataaggcgcg
      961 cgcctcgaca attccctgtc cagttgagat ccgagtttaa tgtttgcttc tatcctctgc
     1021 tgtacatctc ggggcggttc cttctcatgg cggctcgcca acgaatccag gtcgtccatg
     1081 ggcctggtca gggccgtggt ctgcccatcg gggattttgt ctatgttgtg gagcatattt
     1141 gctctcagtt tttctctctc ctctgggtct ttcacatctt gcagcatgcg atcaaccgta
     1201 ttgccgaggg tcatgttctt gaaagattcg cgttcttgtt ggctaccgta gttgcctatc
     1261 aggtcttctt gtctggtcat gttggcaata gtggtgtagt agagctgctc gtaaggcgcc
     1321 gtgacggcgt cgaggcgctg cttctcatcg gcatctttgc cggccgcatc gccactgtcg
     1381 tataggtcga tacggttgag cttctgccca tcgctattgg gtggtgcctg tcggaccagg
     1441 tcgatgtctt ttacattgcc gtccttgttg tcgagtctga catcgagaat gatgcgattg
     1501 ccgggattag cgatgttggc gtcgagaaca gagctgttca agtcttgtac aagcttcagc
     1561 ctctccgcat ttgacatgct gctaatttgt tctttaaagg ctgagccgtc cccacccgac
     1621 gcgacatctt cgacgagctg tttgggcagg tcgtcaggtt tttgcgaatg atccatagct
     1681 ggaaaagcct cttaaattca ggtgtccgtg gtcctgtgag cacagggcgg gccgaccctg
     1741 ttagggcggg gtggcaggct ggccctgagg gcggggtggc gggctggccc ctggtcgagg
     1801 tggcggggca ctcagtctat atgcccccgg caggaggctt ttaagcctga acctggaatt
     1861 acccctggag cttttaatgt ttgacccgaa agctagttct gtgtgggctg atgttaaact
     1921 gacggaagcc aagcaggtgt gccgtgaagg ctgtttccat tgaaaggtag taaggataat
     1981 gtttgaggcc gttctcttcg attttgacgg taccctggta cccagtctcg actactggtt
     2041 gagcggattt aagcatgcct ttgccgagtt ggggcacgag gtttcggagc aagagatcat
     2101 cgagggttgt ttttacaaag acgattctct agtggcggag gctttcggca ttgagtctca
     2161 caagaccttc tggcagttgg tgcagcataa gattgttatg cactacgatt cggctgctct
     2221 ggttcctggt gctagagaag tgctcgactt ttgccgggat cggaatttgc ccctggccct
     2281 ggtcacttcc
//
LOCUS       DAOQNZ010000636         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29124_length_2288_cov_3.343932, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000636 DAOQNZ010000000
VERSION     DAOQNZ010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29124_length_2288_cov_3.343932"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2288)
                     /gene="dnaE"
                     /locus_tag="PKW73_11530"
     CDS             complement(<1..>2288)
                     /gene="dnaE"
                     /locus_tag="PKW73_11530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNA73965.1"
                     /translation="QERDQDAGALESSGYSYSELLRFLVYRGALDRYGDLSEKPAYRK
                     QLEHELSLINRLKLAPYFLIMWDIVRFAQSRGIAVQGRGSAANSAVCYCLKITAVDPI
                     SMDLLFERFLSEGRSEPPDIDLDIAHQEREHVLQYVYRKYGRLHAAMVCEVITYRGRS
                     AVRDAAYVLGFSQEQADLLASLVGHCEAAQAAAKLGGFDKSGAESESGEGLLRPYGFD
                     LADRRVQLLLRVVTGLHQLPRHRSIHVGGFVLSAAPIGELVPVEPASMAERTVIQWDK
                     DDLDIVGLIKIDLLGLGMLTMIQEALKLLQGHRRISLDIGKLDMFDENIYRMLQAADT
                     VGVFQVESRAQMNILPKLKPTCFYDIIVSIAIVRPGPIQGNIIHPYLRRRQGLEPVQY
                     LHASLEPILKRTLGVPLFQEQGMKLAVTAAGFTASQADELRKVMSHKRSLEKMSKLCQ
                     ALAVGMQKNGFGQEAIETITHQLRAFASYGFPESHAASFSLLVYASAYLKHYYPAEFY
                     CAMLNAQPMGFYSPASLLRDAMRHGVTVRPVDMAFSAWNCTLELEEVGAKETYALRLG
                     IRFVEGLGSHAGTLIGAALEQGSFRSLTDVVERSGLGASELKLLANAGAFESFVKGRR
                     KALWQVLSLLKPRRPTPLLDYLVSEDNYLPCSGSGGSSGSSSDTISILGSSIGCDFDL
                     DPDASGALPVMSEVEEVMADFATMQLSTGPHPMSFYRQWAAERQIYSCGGLRTGMDGD
                     DVEVAGGVICRQRPETAKGFVF"
BASE COUNT          624 a          681 c          537 g          446 t
ORIGIN      
        1 ggaaaacaaa acctttggcg gtctcggggc gctgacgaca aatcacaccg ccggcaactt
       61 ccacatcatc accatccata ccggtgcgca acccgccaca agaataaatt tgccgctccg
      121 ccgcccattg ccggtagaag ctcatgggat gcggtcccgt actgagctgc atggtggcaa
      181 aatcagccat cacctcctct acttcactca tgacaggaag agcaccacta gcatccggat
      241 ccaaatcaaa atcacagcca atgctactgc caaggatact gatagtgtca ctgctactgc
      301 cactgcttcc gccactgcca ctgcacggca aataattatc ctcactgacc agataatcaa
      361 gtaaaggcgt aggtcgacgc ggcttgagaa gcgaaagcac ttgccagaga gcttttcttc
      421 gccccttcac aaaactctcg aaagcaccgg cattagccag aagtttcagc tcggaagcac
      481 caagaccgct tcgttccacc acatcggtaa gactgcgaaa ggaaccctgt tcgagcgctg
      541 cacctattaa agtaccggca tgggaaccca gaccctcaac aaatctgata ccaagccgca
      601 aagcatacgt ttctttcgca ccgacctcct caagctcaag agtacaattc caggcactaa
      661 aagccatatc caccgggcgg accgtaacac catgacgcat ggcatctcgg agcaaagacg
      721 ccgggctgta aaaccccata ggttgtgcat tgagcatggc acaataaaat tccgccggat
      781 agtaatgctt taagtaagcg gatgcataaa caagcaagga gaaagaggcc gcgtggcttt
      841 ccggaaaacc ataactggcg aaggcccgga gctgatgagt aatggtctca attgcttcct
      901 gtccaaagcc gtttttctgc ataccgacag ccagagcctg acagagcttt gacattttct
      961 caagagaacg cttatgactc atcacctttc tcagctcatc agcctgagat gcggtgaaac
     1021 cagcagctgt aacagccagc ttcatgccct gctcctgaaa cagcggcaca cccagggtgc
     1081 gcttcaaaat cggctccagg ctggcatgca gatactgcac cggctccaag ccctgccttc
     1141 tgcgcaaata tggatgaata atattgccct gaataggacc gggtcgaaca atagcgatgg
     1201 aaactataat gtcatagaaa caagtgggct taagcttggg caagatgttc atctgcgccc
     1261 gcgactcaac ctgaaagacc cctacggtat cagcagcctg cagcatgcgg taaatattct
     1321 cgtcgaacat atccagcttg cctatatcaa gggaaatccg acggtgccct tgcagaagct
     1381 tcagtgcttc ctgaatcatg gtcagcatac caagaccaag cagatcgatt ttaattagac
     1441 cgactatgtc gaggtcatcc ttgtcccact gaatgacggt acgctcagcc atggaagccg
     1501 gctccaccgg caccagctcg cctatggggg cagcagaaag aacaaaacca cccacatgga
     1561 tagagcggtg ccgcggcaac tgatgcaacc ctgttaccac tcgcagcagc aattgcaccc
     1621 gtctgtctgc caggtcgaaa ccatagggtc gaagcagacc ctccccactt tcagactccg
     1681 ccccggattt atcaaacccg cctagcttgg cagcagcctg agccgcctca caatgtccaa
     1741 ccagagaagc taagaggtca gcctgctcct gggaaaaacc cagtacataa gcggcatccc
     1801 gcacagccga acgccccctg taagtaatca cctcacagac catagcggca tgcaagcgcc
     1861 catacttgcg gtaaacatat tgcagaacat gctcgcgttc ctggtgcgca atatcgagat
     1921 cgatatcagg cggttcgctg cgcccctctg aaagaaagcg ctcgaacaga agatccatgg
     1981 aaataggatc taccgccgta atcttcaggc aataacagac ggccgagtta gcggcagagc
     2041 cacgtccttg cacggcaatg ccgcggctct gcgcgaaacg tacgatgtcc cacataatga
     2101 ggaaataagg agccagcttg agtcgattga tgaggctcaa ctcgtgctca agctgttttc
     2161 ggtaggctgg cttctcactc agatcgccgt aacgatccag ggccccgcga tagaccagaa
     2221 agcgaagcaa ttcgctgtag ctatagccgc tcgactccag tgcaccagca tcctggtcgc
     2281 gctcctgt
//
LOCUS       DAOQNZ010000637         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29168_length_2286_cov_4.614074, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000637 DAOQNZ010000000
VERSION     DAOQNZ010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29168_length_2286_cov_4.614074"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="PKW73_11535"
     CDS             complement(<1..1806)
                     /locus_tag="PKW73_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNA73966.1"
                     /translation="MFERFTEKAIKVIMLAQEEARRLGHNFVGTEQILLGLIGEGTGI
                     AAKTLKSMGVNLKDARTEVEKIIGRGSGFVAVEIPFTPRAKRVLELSWDEARQLGHNY
                     IGTEHLLLGLIREGEGVAARVLENLGVDLTKVRSHVIRLLGESGAATSGGGSSSSSTS
                     TGRSKTPTLDEFGVNMTQAAQENRLDPVVGREKEIERVIQILGRRTKNNPVLIGEPGV
                     GKTAIAEGLAIRISNADVPEILLDKKIVMLDIGLLVAGTKYRGEFEERLKKIMDEIRS
                     AGNVMLVIDELHTLIGAGAAEGAIDAANILKPALARGELQCIGATTMDEYRKHIERDA
                     ALERRFQPVIVDPPSVEETIEILRGLRPKYEEHHRLIISDEAIEHASKLSDRYISDRF
                     LPDKAIDIVDEASSRVRLRASSLPPEGKEVEKELRKVRRDKEMAIRNQEFEKAANLRE
                     SETKLSEKIREIQTAWKAQQETEKPVVTGEEVAYVVSSWTGIPLLKLTEGESERLLHM
                     SDVLHQRVIGQNDAVEAVCKAIRRARVGLKNPNRPIGSFIFSGPTGVGKTELAKALAG
                     YFFGSEDNLIRIDMSEFMEHHTTSKLIGSPPGYVGY"
     gene            complement(2015..>2286)
                     /locus_tag="PKW73_11540"
     CDS             complement(2015..>2286)
                     /locus_tag="PKW73_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HNA73967.1"
                     /translation="PRAGCGGFIVEKKSRHGKVFWGCSNYGANECTSAFWYPPLTGGG
                     PGGSNFCPKCNSMLIYKTLKRGDQIACSSKTCDFAQLITGQETHA"
BASE COUNT          515 a          620 c          610 g          541 t
ORIGIN      
        1 gtagcccaca tagccgggag gagaaccaat caacttggaa gtggtgtggt gctccatgaa
       61 ctcggacatg tcgatacgaa tcaagttatc ttcagaaccg aagaagtaac cggccagagc
      121 cttagccagt tcggttttac caacgccggt aggaccggag aagatgaagg aaccgatggg
      181 acggttggga ttcttcaagc cgacgcgggc gcgacgaatc gctttgcaaa ctgcttctac
      241 cgcatcgttc tgaccgatga cgcgctggtg caacacatca gacatgtgca agaggcgctc
      301 agattcacct tcagtcaact tgagcagcgg aatgccggtc caggaactga ccacgtaagc
      361 aacctcttca ccggttacaa ccggcttctc ggtttcttgc tgggccttcc aggctgtctg
      421 aatttcgcga attttctcgg agagtttggt ctccgattct ctcaaattgg cggctttctc
      481 aaattcctga ttgcgaatag ccatttcttt atcgcgccga actttgcgca attccttctc
      541 aacttctttg ccttccggcg gcaagctgga agctctcaag cgcacccgcg atgaagcttc
      601 gtcgacgatg tcgatggctt tatcgggcag gaagcggtcg gatatgtagc gatcggaaag
      661 tttgctggcg tgctcgatgg cttcatcgga gatgatcagc ctgtggtgct cttcgtactt
      721 gggacgcaga ccacggagaa tttcgatggt ttcttccacc gagggcggat cgacgattac
      781 cggctggaaa cgacgctcga gggcggcatc tctttcgata tgcttcctgt attcgtccat
      841 ggtggtagcg ccgatgcact gcaactcgcc gcgggccagc gccggcttga gaatgttggc
      901 ggcatcgatg gcaccttcgg cggcaccggc accaatcaag gtgtgcaatt cgtcgatgac
      961 gagcatgacg ttgccggcgc ttctgatctc gtccatgatc ttcttgaggc gctcttcgaa
     1021 ttcgccgcgg tatttggtac cggctactag aagaccgata tccagcatga cgattttctt
     1081 gtctagaagt atctccggca catcggcgtt ggagatacga atggccagac cttctgctat
     1141 ggcagtttta ccaacaccgg gctcaccgat gagcaccgga ttgttcttgg tgcggcgtcc
     1201 caaaatctgg ataacgcgct cgatttcttt ttcacgaccg accaccggat caagccgatt
     1261 ttcttgagca gcctgggtca tattgacgcc aaactcgtca agggtagggg ttttggaacg
     1321 accggtggat gtagagctgg aggaagaacc accgccggag gttgccgcac cggactcacc
     1381 gagaaggcga attacatggc ttcttacctt ggtcaggtcg acgccgagat tttccagcac
     1441 tcgggcagca acgccttcgc cttcgcgaat cagaccgagg agcaaatgct cggtaccaat
     1501 atagttatga cccagttgcc gggcttcatc ccaggaaagc tccaggacgc gcttggcgcg
     1561 gggtgtgaag ggaatctcta cagcaacgaa gccggagccc ctgccgatta tcttttccac
     1621 ttccgttctg gcatctttca aattgacgcc catcgacttg agggtcttgg cagcaattcc
     1681 agtaccttcg ccaatcaggc caagtagaat ttgctccgtg cccacaaaat tgtgaccaag
     1741 ccggcgcgct tcttcttgag cgagcatgat gaccttgatc gccttttccg tgaacctttc
     1801 aaacatggtg gtttaagccc tcttagccgc gaatgtcctt acatttagcg ctggttccct
     1861 cttgactcgg agaaccgtcc gcaaatgtga ttacattcta acaccatgca aacctggttt
     1921 ttctggcaag ccaagccaat ttcagcaaat tactgctgaa gaaatcatga agatggtcac
     1981 aaagcctgac aggcaggcta gaaagcgacc gcaatcaagc gtgggtttcc tggccggtaa
     2041 tgagctgtgc aaaatcacaa gtcttagacg agcaggcaat ctggtcgccg cgcttcaaag
     2101 tcttgtaaat aagcatgctg ttgcacttgg gacagaaatt agagccgccc gggccgccgc
     2161 ctgtcagggg cggataccag aaggcactcg tacattcatt ggcgccgtaa ttgctgcaac
     2221 cccagaaaac tttgccgtgg cgagattttt tctcaacaat gaagccgcca caaccagccc
     2281 gcgggc
//
LOCUS       DAOQNZ010000638         2285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29209_length_2285_cov_3.080269, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000638 DAOQNZ010000000
VERSION     DAOQNZ010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29209_length_2285_cov_3.080269"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKW73_11545"
     CDS             complement(<1..400)
                     /locus_tag="PKW73_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73968.1"
                     /translation="MPLPNESDFETHSQSLAEYLVLVMLVLMVAVPVSGFVLSAQSWV
                     SSLVAKGAYQVEDTKNLAEQLFDASAKIRDLERKLAKNELELTRLKQESRDTNRLRSL
                     LDLKQKSQRQTVAAEVVSRSPDNWFEQVVID"
     gene            complement(430..1464)
                     /locus_tag="PKW73_11550"
     CDS             complement(430..1464)
                     /locus_tag="PKW73_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNA73969.1"
                     /translation="MAFFNNIFWHGIGIDLGTANTLIYVHDKGVVLREPSVVAVDENT
                     NMVRWVGEEARAMIGRTPQGIKAIRPLKDGVIADLEHAKVMLRRFVERVTGAGGIKNP
                     DMAIGVPSGVTSVERIAITAAAQNARASRIYLPEESMAAAIGASLPVVEPTGSMIVDI
                     GGGTTEVAVISLSGIVVNESIRIAGDELTESIITYLKKVHSLAVGERTAEEIKFRLGS
                     AFKTSDNERLDVRGLSLLNHVPRTITISRGEVRESMQEPLKAIVEAVKRTLERTPPEL
                     AADIFNRGIFITGGGALLSGLDELISHETEVPVFVADDPLSSVAIGTGKMLTDPMYRR
                     VLEQCLSRKS"
     gene            complement(1565..1786)
                     /locus_tag="PKW73_11555"
     CDS             complement(1565..1786)
                     /locus_tag="PKW73_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73970.1"
                     /translation="MVAENKLPSVSQLQKLVREKTPVEIICLDGKSSRFSGLLKWYDG
                     EAFHLKQEDGSEITILRSAVLGYRVKKAK"
     gene            complement(1786..>2285)
                     /locus_tag="PKW73_11560"
     CDS             complement(1786..>2285)
                     /locus_tag="PKW73_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73971.1"
                     /translation="KSLYSVRMYTDLTSLWYGWTKNLYSFIDSKPSMLILILVMINSV
                     VVAPWLWLGFIGWQWAAGEVSELLLRMTLLSLVQFGTIALWLKRTSCHHAGLKWYHFF
                     LTPVGCLAVSALYIHAAYLVHSGGQVNWKGRRYVVNTRRTIEPQDGEREAGLEGKLVS
                     RDVAD"
BASE COUNT          552 a          636 c          606 g          491 t
ORIGIN      
        1 tatctataac tacctgctca aaccagttgt ccggactgcg ggagaccact tccgccgcca
       61 ctgtttgccg ctgcgacttc tgcttcagat caagcaggga gcgcaaacga ttggtatccc
      121 tggattcctg cttgagacgg gtcaactcca gttcgttttt ggccagctta cgctccagat
      181 cgcgaatctt ggcagaggca tcaaagagtt gctcggctaa attcttggta tcttccacct
      241 ggtaggcgcc tttggccaca agggaggaga cccaactctg ggccgagaga acgaagccgc
      301 tcaccggcac tgccaccatg agcacaagca tgaccagcac aaggtattca gctagtgact
      361 gagagtgagt ctcaaaatcc gactcatttg gcaggggcaa atctgtctaa gtcctcgctg
      421 agtaaaaacc taagacttac ggctcaggca ctgttccaga acccttctgt acatggggtc
      481 ggtcaacatt ttgcctgtgc ctatggctac cgaggagagg ggatcgtcgg cgacgaaaac
      541 cggtacttct gtttcgtggg agataagctc atcgaggccg gagaggaggg cgccaccacc
      601 ggtaatgaag ataccgcggt tgaaaatatc agcggccagt tcggggggag tgcgctccag
      661 agtgcgcttc accgcctcaa cgatggcttt gaggggctcc tgcatggatt ctctcacttc
      721 accgcggctg atggtaatgg tgcgcgggac atgattgagc aagctcaaac cacgcacatc
      781 aagacgttcg ttatcgctgg ttttgaatgc cgagccgagg cgaaacttga tttcttcagc
      841 cgtgcgctca cccacagcca ggctgtgtac tttcttgagg taggtgatga tggactcagt
      901 cagttcgtca ccggcgatgc gaatggactc attgaccact atgccggaaa gactgatcac
      961 ggccacttcc gtggtaccgc cgccgatatc gacgatcatg gaaccggtgg gttccaccac
     1021 aggcagggaa gcaccgatgg ccgctgccat ggattcttcc ggcaaataga tacgagaggc
     1081 gcgggcattc tgggcggcag ccgtgatggc gattctctcc accgaggtca ccccggaagg
     1141 aacaccaatg gccatatcgg ggttcttgat gccgccggca ccggtcactc tctcaacgaa
     1201 acgtctgagc atcactttgg catgctcaag atcggcaatg acaccatctt tcaagggtcg
     1261 aatcgccttg atgccttgag gggtgcgacc aatcatggcc cgggcctctt ccccgaccca
     1321 acgcaccata ttggtgttct catccaccgc caccacggaa ggctctctca agacaacgcc
     1381 tttgtcgtgc acgtaaatga gcgtgttggc cgtaccaagg tcgataccta tgccatgcca
     1441 gaaaatattg ttgaagaaag ccactgacta actcccatag atacgggtga tatatcaacg
     1501 cagatatgca acgaagatat aggaggagag cacttaaaac gccgagccag gctttagttt
     1561 aacactattt ggctttcttt actctatatc ccagaaccgc cgaacgcagg atggtaatct
     1621 ccgaaccgtc ctcttgtttg aggtggaaag cctcgccgtc ataccacttc aaaagcccgg
     1681 aaaagcggct gctcttgccg tccagacaaa tgatctccac aggagtcttc tctcttacca
     1741 acttctgcaa ctgcgaaacg gaaggtagtt tattttcggc caccatcagt cggcaacatc
     1801 tctggaaaca agcttgcctt caagaccggc ttcccgctct ccatcttgcg gttctatagt
     1861 gcggcgcgtg ttgaccacat agcgccgccc tttccagttg acctggccgc cgctatgaac
     1921 aagataagca gcatgtatat agagggccga aactgccaga cagccgaccg gcgtcaggaa
     1981 gaagtggtac cacttcagcc cggcatggtg gcagcttgta cgcttgagcc agagagcaat
     2041 agtgccgaac tgcaccaggg aaagcagggt catgcgcaaa agcagctcgg agacctcgcc
     2101 ggcggcccat tgccagccta taaagccaag ccatagccag ggagccacca ccaccgagtt
     2161 gatcatgaca agaatcagga tcaacatgga gggcttgctg tcgataaagc tatatagatt
     2221 tttggtccaa ccgtaccaga ggctggtcag gtcggtgtac atgcgcacgc tgtaaagaga
     2281 cttac
//
LOCUS       DAOQNZ010000639         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29314_length_2281_cov_2.835130, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000639 DAOQNZ010000000
VERSION     DAOQNZ010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29314_length_2281_cov_2.835130"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKW73_11565"
     CDS             <1..964
                     /locus_tag="PKW73_11565"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNA73972.1"
                     /translation="LKGEKSVRIPRVYWKLTGRRVLTLEYLPGTKINQVEEIKKQGID
                     PVAIGNNLVAAYMEQVLTAGFFHADPHAGNLAIGPDGRIVIYDFGMVGEISQTQRESI
                     LGCIASVINFNAVELIKNLVSLGIVKEDAKHESMIRAVQPFIEYYKGKQVKELDFTSL
                     EHDIDQIAMDRALKLPPSLAYLLRTASSLEGIARSLHPNFSFVEAAKPSVKKWALANP
                     AQAYLALGYLTQGKLFGQRGIFSEEGVLRLFQTKSQEPTGATDRKGTARSKTDNQADS
                     VTSTSTTLSTVRSLKGAPPKYLKSIRPSNNPSGNYLSDFKRFEN"
     gene            1228..1992
                     /locus_tag="PKW73_11570"
     CDS             1228..1992
                     /locus_tag="PKW73_11570"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNA73973.1"
                     /translation="MLKWRAAARTDAGCQRQRNEDNYYVSPDNRVFAVADGMGGAVGG
                     ARASKLAMDAVEKQWQEAAPPSTDKEAIKVWLTKTVNEANSAVWNSAEEDQSVRGMGT
                     TIVVAVQSEDNYMQIAHVGDSRAYLYREGKIKLLTNDHSVVQEMVRAGRLTEEQARIN
                     PYKNLITRCLGHEERVEIDHTPLEMKPMDWIVLCSDGLPTVLRDEQIGDVLGAGVEPE
                     SVCEELVKQTIDGNAPDNVTVVVVKYLDEGSDNGSK"
BASE COUNT          619 a          595 c          599 g          468 t
ORIGIN      
        1 cctgaaaggc gaaaagtccg tgcgcattcc cagggtttac tggaaactga ccggacgtcg
       61 ggttcttacc ctggaatatt tgcccggcac caagatcaac caggtagaag aaatcaagaa
      121 gcaaggcata gatccggtgg ccatcggcaa caatcttgtg gctgcttata tggagcaggt
      181 actcacggcc ggtttcttcc atgccgaccc ccatgccggc aatctggcca tcggtcccga
      241 cgggcgcatt gtcatctatg acttcggcat ggtgggggag ataagtcaga cccagagaga
      301 atctatattg ggttgcatcg cttcggtaat taatttcaac gccgtggagc tgataaaaaa
      361 cctggtcagc cttggcatcg tcaaagagga tgccaaacat gaatccatga tccgggcggt
      421 tcagcctttc atcgagtatt acaagggcaa gcaagtcaag gagctggatt tcaccagcct
      481 cgagcatgac attgaccaaa tagccatgga ccgcgccctc aagttaccac cctctctcgc
      541 atacctgctg cgcaccgcct cctctctgga gggcatagcg cgctccctcc accccaattt
      601 cagcttcgtg gaagcagcca aaccctcggt caagaagtgg gcccttgcca acccggccca
      661 ggcctatctg gctcttggct atctcaccca gggcaaactc ttcggacagc ggggtatttt
      721 ctcggaagag ggagtattga ggctcttcca gactaaatcg caggaaccga ccggagctac
      781 cgaccggaaa ggcacagccc gaagcaaaac cgacaaccag gccgactccg tgaccagtac
      841 atcaactacg ttaagtactg ttcgctcttt gaaaggggca ccgccaaaat atctcaagtc
      901 aattcggccc tcgaacaatc cgagcgggaa ctatctttca gacttcaaga gattcgaaaa
      961 ttagaagctc atttatatat tttgaagaga gaagttaaga ctctcaggaa aaatagagta
     1021 aagttattag tcacgattct tgccgcctcc acactatctc ttagctactg gagtctcaca
     1081 ccaagaaccg gaagccctca atattccgag ttttttttga ttggaaatgg tgtaatggtg
     1141 gcaattatta tttggcaatt ggtaagacca cctgatttgg cctcccgaaa gcaagccgat
     1201 agatcaagag gtagacggag atagacaatg ctgaaatggc gtgcagcggc acgaactgat
     1261 gcgggttgcc agcgacagag aaacgaggac aactattacg taagcccgga caaccgagtg
     1321 tttgccgtgg cggacggcat gggtggagcc gttggcggcg ctagagccag caagctggct
     1381 atggatgctg ttgaaaagca atggcaggaa gcagcccccc cctctaccga caaagaggca
     1441 attaaggtct ggttgaccaa gacggtcaat gaagccaaca gcgccgtctg gaactccgcc
     1501 gaggaagacc agagcgtgcg aggaatgggc accaccatcg tggtggctgt gcaatccgaa
     1561 gacaactaca tgcagattgc ccacgtgggc gactctcgcg cttaccttta ccgggaaggc
     1621 aagatcaaac tacttaccaa cgaccattcc gtcgtacagg aaatggttcg ggccgggcga
     1681 ctgactgaag aacaggcgcg catcaaccct tacaagaatt taatcactcg ctgtctgggg
     1741 catgaagagc gggtcgaaat cgaccatact cctctggaaa tgaagcccat ggactggatc
     1801 gtgctctgtt ccgacggtct gccgacggtt ctgcgcgacg agcaaatagg agatgtcctg
     1861 ggagcaggag tggagcctga atcagtctgc gaagaactgg tcaagcaaac catcgacgga
     1921 aatgctccgg acaacgtcac agtagtagtg gtcaagtacc tggacgaggg ttcggataac
     1981 ggctccaaat agggtacggt ttcaccaggt tggaagcagg ggctggcgag taaattgcac
     2041 ataccctgct accgggcttg gtacaatttg aagtgaatta gaccgaccga acatgaagga
     2101 tctactcgag acaaggggct cacaggatgc gatcggcatt tctcttcaac aaacttacta
     2161 acgaacgaca tcccctcaac caatccgtaa gcaagatcgg aagagaccag actaacaata
     2221 tatccattgc ctccgatcat tatgtctcca gacaccatgc ctgggtattg cagatgcagg
     2281 g
//
LOCUS       DAOQNZ010000640         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29368_length_2278_cov_4.201080, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000640 DAOQNZ010000000
VERSION     DAOQNZ010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29368_length_2278_cov_4.201080"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKW73_11575"
     CDS             <1..581
                     /locus_tag="PKW73_11575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA73974.1"
                     /translation="KSKMVLSAVGRLVGLVNDLLDIEKMESGNLVLKIRPTPVDRIVE
                     GSLELVSGFAEQQGVRLESVDLPQVEAAVDEDRIVQVLQNLLVNAVKFSPEGKAVTLA
                     VVPAVGPGGAGMLRFSVKDRGPGIKPEELGSLFERFIQLEGADGKRRGGTGLGLAICK
                     EIVKKHGGRVGVESTPGKGSEFWFEVPVSQPG"
     gene            706..1395
                     /locus_tag="PKW73_11580"
     CDS             706..1395
                     /locus_tag="PKW73_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA73975.1"
                     /translation="MAKILIAEDDRLVAGMLKDLLELDQHTVELAHNGRDALDLVCSF
                     PYDLLILDLGLPEMEGTEVLNRYRGRGGKLPVLILSGRNDVNHKIEGLDLGADDYMTK
                     PYDVRELNARIRSLLRRQFVADVVSNVLKVGHLEMDTARCEVLKSGEKLKLLPTEYAL
                     LEFFMRNRGRVFSASELLERIWKSDSDASENAVRTYITRLRKKIDEEGKASLITSVYG
                     LGYKMEEVDNR"
     gene            1779..>2278
                     /locus_tag="PKW73_11585"
     CDS             1779..>2278
                     /locus_tag="PKW73_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNA73976.1"
                     /translation="MQNSSEAQSSGANLDQAAPFAVAPPLTFQDLPGLQMKCCTRNSG
                     DSRNSSGDSQRVYLFREAFSAFAGEAGLSAASRDQMKARLGGKGAGLAEMTASGVNVP
                     PGLTITTDTCLDYYRGGQVMPAGLMEEVTVKLSQVEARVGRRLGDAALPLLVSVRSGA
                     KFSMPG"
BASE COUNT          482 a          605 c          637 g          554 t
ORIGIN      
        1 gtaagtcgaa gatggttctt tcggcggtgg ggcgcctggt gggcctggtg aatgacttgc
       61 tcgacattga aaaaatggaa tccggcaatc tggttctgaa aatacgtccc accccggttg
      121 atcgcattgt agagggctca ctggagctgg tctcaggctt tgcagagcag caaggcgtca
      181 ggttggaaag tgttgatttg ccccaggtgg aagcagccgt ggacgaggat cgcattgtgc
      241 aggttttgca gaatttgctg gtcaatgccg tcaagttttc ccccgaagga aaagcagtca
      301 cccttgccgt tgttccggcg gtgggaccag gtggtgccgg catgttgcgt ttcagcgtta
      361 aagaccgcgg cccgggtatc aaacccgaag agctaggatc gctctttgag cgtttcatcc
      421 agttggaggg cgccgatggc aagcgcaggg gcggtaccgg tctggggctt gccatttgca
      481 aggaaatagt gaagaaacac ggcggccggg tgggagtgga aagcacaccg ggcaagggta
      541 gtgagttctg gttcgaggtg ccggtcagtc agcccggcta atgcctgcca gtaattctat
      601 aaatctgcta accttttcct tctggaaggg aagtcttcag ccggccccgg caaaagactt
      661 taccgaggca actaccccct gaattagacc cgccggtagg agaccatggc caagatcttg
      721 attgctgaag atgaccgtct ggtagcgggc atgctcaagg atttgctgga attggaccag
      781 catactgtgg agctggctca taacggcagg gatgccctgg atctggtctg ttctttcccc
      841 tatgacctct tgattcttga cctgggtctg cctgagatgg agggtaccga ggttctcaac
      901 cgctaccggg ggcgaggcgg caagttgccg gtcttgattc tctccggacg caatgatgtc
      961 aatcacaaaa tcgaaggtct cgatttaggc gccgatgact atatgaccaa gccatacgat
     1021 gtgcgggagt tgaacgctcg cattcgttcg cttttgagaa ggcaatttgt ggcggatgta
     1081 gtgtccaatg tactgaaagt tgggcacctg gagatggaca ctgctcgctg cgaagtcttg
     1141 aaatccggcg agaagctcaa gcttctaccg accgaatacg ctcttctgga gtttttcatg
     1201 cgtaatcgcg gccgtgtatt cagtgcgtcg gaactgctgg agaggatctg gaagtccgat
     1261 agtgacgcct cggaaaatgc cgtgcgcacc tacattacac gcctgcgcaa gaaaatcgat
     1321 gaggaaggta aggcttctct cataacttct gtatatggtc ttggttacaa aatggaagaa
     1381 gttgacaacc gttgactttg cttctgctgc ccatggttat caaggtctca ttcagattct
     1441 cttcgtttac atgtctaaaa gactctgcca gcttggctaa ttccagtttg gatcaatagc
     1501 aaattgctta tacacaggct gttacttagg cggcatctga acggtgccac cgcttaagct
     1561 acaggcgtca atttcatcta gtttttcaga agccctgtct taactcagcc agccaccacc
     1621 ccctcatcat ccagctttct gtgcccgatc ttgtggtacc tggtttgaaa tcggctgctg
     1681 tttcgacttg cctaacccta acttcctttc cccggttaca agtcggaaag ggcttctgcc
     1741 tccacccaac tattctcaaa tggaaacgga gaaaacccat gcaaaactct tctgaagcac
     1801 agtcgtccgg tgccaatctc gatcaagctg cacccttcgc tgtggcgccc cctctcacgt
     1861 ttcaggacct acctggtctg caaatgaagt gttgcacccg caactctggc gattcccgga
     1921 actcttctgg cgactcccag cgggtctacc tcttccgcga ggctttcagc gccttcgccg
     1981 gcgaagccgg tttgagcgcc gccagccgcg atcagatgaa agcccgtctg ggcggtaaag
     2041 gcgccggtct tgcagaaatg actgcttccg gcgtaaacgt tccgccaggc ttgaccatca
     2101 ccactgacac ctgcctggac tattaccggg gcggacaggt tatgcctgcc ggtctcatgg
     2161 aggaagtgac ggtcaagctc agtcaggtcg aggcccgtgt cggtcgtcgc cttggcgatg
     2221 ccgccctgcc ccttcttgtt tcggtgcgct ccggcgccaa gttctccatg cccggcat
//
LOCUS       DAOQNZ010000641         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29453_length_2275_cov_3.669369, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000641 DAOQNZ010000000
VERSION     DAOQNZ010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29453_length_2275_cov_3.669369"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKW73_11590"
     CDS             <1..232
                     /locus_tag="PKW73_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73977.1"
                     /translation="VGTRTMLTARGYYHDYQGQPNNYYKGVFFDASPAEPRAKIEEIY
                     LQSKNPEIFEKYLPAFDRCLATIQWLSVIEAK"
     gene            233..1675
                     /locus_tag="PKW73_11595"
     CDS             233..1675
                     /locus_tag="PKW73_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73978.1"
                     /translation="MIPSFFLLLLYMLLVAPMGFLRDGAAARHIVSGLYILEHGRPAT
                     SNYLWYVDPLAPWQTHELLTDFTLGCLYRLTGEYQLVVLAAAVAMALAISLAAQSAIK
                     SGRCPMLSLAPLVVLAATATSLHWSARAHIFSYVFLSLTMGLARSKLSTGKLSGLLFL
                     LFAAWVNFHGSAILGLPLVLALTLARQAEQKAAPTPTSKPFLACLPLLAALLGCLSSL
                     RGMHYFSFLLQYATQGQILGQGSEWTGIDTAYGIGTWAYLILALIFLANCVKNWKQYR
                     RPDLLPLTLVTLGFAMTALPCMRIIPYASLLMLSMFEAKAKPEERKEEEPSAHAGKWK
                     GASPLLLSLTAILILSFAATAHIKDMNPHFLPVRCSNFLKDNCNKETRGFAYDNWGNY
                     LYIKCGQSFFIDDKTEFYPKDFNTAYRALLLAQSGYQDFIKEARFPFILIPRQAPLAE
                     ALEASGKWRIAERDEDAYLFMPVQAPLSTP"
     gene            complement(1641..>2275)
                     /gene="larB"
                     /locus_tag="PKW73_11600"
     CDS             complement(1641..>2275)
                     /gene="larB"
                     /locus_tag="PKW73_11600"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNA73979.1"
                     /translation="VVFAPGKSSEQIFQIALELRQAHDVVLASRAEAEQARSVLLLAQ
                     EQGLSDVKYHQSAAVISFGVFPQVERGSEDAAEVAVLTGGTVDIPVAEEALVVLESAG
                     ISCRRYFDMGVAGLHRLLGVLDSVRKSRVVLVVAGMDGALPSVVCGLVGVPVIAVPTA
                     AGYGTGLGGLAALASMLNTCAPGLTVVNIDNGLGAAAFAIRVLRGVPEPA"
BASE COUNT          532 a          725 c          547 g          471 t
ORIGIN      
        1 cgtcggcacc aggacaatgc tgacggcccg gggctattac cacgactatc agggacagcc
       61 gaacaattac tacaaaggcg tcttcttcga tgccagcccg gcagagcccc gggccaaaat
      121 cgaagaaatc tatttacaga gcaaaaatcc ggagattttc gagaaatatc tgccggcctt
      181 cgatcgctgc ctggctacga tacaatggtt gtccgtcatc gaagccaagt aattgattcc
      241 aagctttttc ctcttgctcc tctacatgct cctggtagcg cccatgggct ttcttcgtga
      301 cggcgccgcc gcccggcaca ttgtcagcgg gctctacata cttgagcacg gaagaccggc
      361 tacaagcaat tatctctggt atgtagatcc acttgccccc tggcaaacac acgagttgct
      421 gacagacttc actcttggct gcctttatag actaacgggc gaataccagc tcgtggtcct
      481 ggctgctgca gtagccatgg cgctggccat cagccttgcc gcccaaagtg caatcaaaag
      541 cggccgatgc cccatgctaa gcctcgcacc gctggtagtg ctggctgcca ctgccacctc
      601 cctacactgg tcggcacgag cacatatctt ttcctacgtc tttctctcat taaccatggg
      661 gcttgccaga agcaagctca gcacaggcaa attgagcggc ctgctatttc tactatttgc
      721 agcctgggta aacttccacg gcagtgccat cctcggtcta cctctggttt tagccctgac
      781 actggcccgc caggcagagc aaaaggcagc gcccaccccg acctcaaaac cgtttcttgc
      841 ctgcttacct ctgcttgccg ccctgctggg ctgcctgagc agcctgcgcg gtatgcacta
      901 tttcagcttt ctacttcaat acgccaccca gggacaaatt ctcggacagg gctcggaatg
      961 gaccggcatt gataccgctt acggtatcgg cacctgggcc tatctgatac ttgctctaat
     1021 cttcctggcc aactgcgtga aaaactggaa gcagtaccgc cgccccgact tattgcccct
     1081 caccctggtc accctgggct ttgccatgac ggccctgccc tgcatgcgaa tcattcccta
     1141 tgcttcactg ctcatgctct caatgttcga ggcaaaggca aagccggaag aaagaaagga
     1201 agaggaacct tccgcccacg ccgggaaatg gaaaggcgct tcaccgctgc tcctcagcct
     1261 gactgccata ctgatactgt cctttgccgc cacagcccac ataaaggaca tgaatccaca
     1321 ttttctgccg gtacgttgca gcaattttct caaagacaat tgcaacaaag agactcgggg
     1381 ttttgcttac gataactggg gcaactatct ttatataaag tgcgggcaga gtttcttcat
     1441 tgacgacaag acagaattct atcccaaaga tttcaacacc gcctatcgag cccttcttct
     1501 tgcccagagc ggttaccagg actttatcaa ggaggctcgt ttcccattta tcctgattcc
     1561 gcgccaggca ccactggcgg aggcccttga ggctagcggc aaatggcgca ttgccgaaag
     1621 agatgaagac gcttacctgt ttatgccggt tcaggcaccc ctctcaacac cctgatagca
     1681 aaagccgccg cccccagacc gttgtcgatg ttgaccacgg tgagaccggg cgcacaggtg
     1741 ttgagcatgg aagcaagggc ggccagacca cccagaccgg taccgtagcc ggccgcagtg
     1801 ggcacggcaa tgaccggaac gccgaccaga ccgcaaacca cgctgggtaa agccccatcc
     1861 atgccggcta caaccagcac cactcggctc ttacgcacgg aatcgagaac tcccagcaaa
     1921 cggtgcaagc cggccacacc catatcaaaa tagcgacggc aggaaatacc ggccgactcc
     1981 aacaccacca gcgcttcttc cgccaccggt atgtctacgg taccgccggt aagaacagcc
     2041 acctcagccg catcctcact gcctcgctca acctgcggga acacgccaaa actaataacg
     2101 gcggcgctct ggtgatactt gacgtcgcta agaccctgct cctgagccag caaaagcact
     2161 gagcgagcct gttcggcctc ggcgcgggaa gccagcacca catcatgggc ctgtcgcaac
     2221 tctaaggcaa tctggaaaat ctgttcgctg ctcttaccgg gagcaaatac cacct
//
LOCUS       DAOQNZ010000642         2274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29478_length_2274_cov_4.488959, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000642 DAOQNZ010000000
VERSION     DAOQNZ010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29478_length_2274_cov_4.488959"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /locus_tag="PKW73_11605"
     CDS             complement(<1..1856)
                     /locus_tag="PKW73_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNA73980.1"
                     /translation="MDSNSGIIRVSIGGPPTVVAPVFIDPVCHMQVEKEKAAASLTYE
                     GTNYHFCHINCAVKFSRNPVQYTGGAETGTLNEAGSKTEADGKIEAAADCGQEEATAT
                     SGAAFTCPMHPEVLTDRQMPCPLCGMALDPLEPTEESDDGELDDMLKRLRLAAAFTVP
                     LAILGMKDMFLPLPALPWQALNYLLLVLSAPVLWQGKPFFERALASLKTGAFNMFTLI
                     GTGVSAAWGYSLFATLFPHLLPAADQHHGIATYFEPAAVIITLALFGQVLELKARKST
                     NAAISELLQMAPKQAHFLLLDDSEVEIDAKKVERGDRLRIKPGESVPVDAIVLSGSSS
                     LDQSMLTGESLPVQKGEGDTIYGGTINGRGSLLVRAIHVGKETLLAQIISLVAQAQRS
                     RAPIQTKVDQVAAYFVPLVFLISAATFLAWLVLTPPARDPLASALLSAVSVLIIACPC
                     ALGLATPMSIMVAIGRAARDGVLVKDAQTLEHLASIDTLVVDKTGTLTEGRFKVVQTR
                     YLNQSVQAEKEGSASKAPALVPVPVTTPALALELAAALESASEHPLAGAIVAHAKSIR
                     NEAAAHETTNRRLQAEAVEAEPGGGITGYVNVTTDGSGQNTAVQARYQVQAG"
     gene            2046..>2274
                     /locus_tag="PKW73_11610"
     CDS             2046..>2274
                     /locus_tag="PKW73_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73981.1"
                     /translation="MREASQPADEPDKDEKRTRFRRRARIFVGLSLVLSTFYVAFFWF
                     DRLFVSPQELYRRAFQAVAENIYDQSRLADMS"
BASE COUNT          438 a          604 c          704 g          528 t
ORIGIN      
        1 ctgccggcct gtacttgata gcgcgcctgc acagccgtat tttgcccgct gccgtcggta
       61 gttacattta catagcctgt gatgccgcct cccggttcag cctctaccgc ctctgcttgc
      121 aggcggcggt tggttgtttc atgggcagcc gcttcgttcc ttatggattt tgcgtgggcg
      181 acaatagcac cggccagagg atgttcgctg gcgctctcca gggcagcggc cagttccagc
      241 gccagagccg gagtcgtaac tggaaccgga accagagccg gggccttgct tgcgcttccc
      301 tctttctcgg cctgaacgga ctgatttaag tagcgggttt gcacaacctt gaagcgtcct
      361 tccgtcaatg tgccggtctt atccaccacc agagtatcta tggaagcaag atgctccagg
      421 gtttgagcgt ctttgaccag cactccgtcc cgagcggctc tgcctatggc taccatgatg
      481 gacatgggag tggccagacc gagggcgcag gggcaggcga tgatcaggac cgagacggcg
      541 ctcaaaagtg ctgatgccag ggggtcccgg gccggtggcg tgagaacgag ccaggccaga
      601 aacgtagcgg cgctgattag aaagaccagg ggtacaaaat aggcggccac ctgatctact
      661 ttggtctgaa taggggcgcg gctgcgctgg gcctgggcca ccaggctgat gatctgggcc
      721 agcagtgtct ctttgcccac atggatggca cgcaccagaa ggctgccgcg gccgttgatg
      781 gtgccaccgt aaatagtatc gccttcgcct ttctgaacgg gcagagactc gccggtgagc
      841 attgactgat cgaggctgct gctgccgcta agcacaatgg catctacggg cacggactct
      901 cccggtttga tgcgcaggcg gtcgcctctt tcaacttttt tagcatcgat ttctacttca
      961 ctgtcatcga gaagcaagaa gtgggcctgt ttgggcgcca tttgcaaaag ttctgagatg
     1021 gcggcattgg tgctcttgcg ggctttcagc tcgagtactt gtccgaagag ggcgagggtg
     1081 ataatgactg cggccggttc gaaatatgta gctataccat ggtgctggtc ggcggcaggc
     1141 aacaggtgcg gaaataatgt ggcgaagaga ctgtagcccc aggctgccga tacgccggtg
     1201 ccgatgaggg tgaacatatt gaaggcgccg gtttttaaag aggccagggc tcgctcgaaa
     1261 aagggcttgc cctgccagag aaccggtgcg gagaggacca ggaggagata gttgagggct
     1321 tgccagggca gtgccggcag cggcaaaaac atgtccttca tgcccagaat tgccagtggt
     1381 acggtaaaag cagcggccag gcgcagtcgc ttcagcatgt cgtccagttc gccgtcgtcg
     1441 ctttcctccg tcggctccag gggatcaagg gccatgccgc aaagggggca gggcatctgc
     1501 cggtcggtca gtacttccgg gtgcatgggg caggtgaagg cggcgccgct tgttgctgtt
     1561 gcttcttcct gtccacagtc tgcagcggct tcaattttgc cgtccgcttc agtcttgctg
     1621 cccgcttcat tcaaggtgcc cgtctcggcg cccccggtat actggacagg attgcggctg
     1681 aactttacgg cgcaattgat gtggcagaag tgataattgg ttccttcgta ggtgaggctg
     1741 gctgcggcct tttctttttc tacttgcata tggcagaccg ggtcgatgaa gaccggcgct
     1801 actactgtcg gcggtccgcc tatgctgacg cgaataatac ctgaattact gtccattgcc
     1861 cggagattgt ttgagcgcct ttgggaacga gttcgctatg ccagactaga gccgtcgaac
     1921 cctgcctttg gtcagcatag agtatagccc agggcgctcc tcgtgacatt gaggaagcgc
     1981 cgtgcatgtt aagcggttaa attattactt ggggcggggc atatagccag agttcagagg
     2041 tgacagtgag agaagcgagc cagccggctg atgaacccga taaagatgag aagagaaccc
     2101 ggtttcggcg ccgggctcgc atttttgtcg gcttatctct ggttctttcc actttctatg
     2161 ttgccttttt ctggttcgat cgtttattcg tctctcccca agaactttat cgacgtgcct
     2221 ttcaggcggt ggctgaaaat atctacgacc agagccgcct tgccgatatg agcg
//
LOCUS       DAOQNZ010000643         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29553_length_2271_cov_3.418773, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000643 DAOQNZ010000000
VERSION     DAOQNZ010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29553_length_2271_cov_3.418773"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            795..>2271
                     /locus_tag="PKW73_11615"
     CDS             795..>2271
                     /locus_tag="PKW73_11615"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNA73982.1"
                     /translation="MNTKTSTDNSCQNEPIEPAPTVGQNNPVKLKDNWCSLENTYRKD
                     DQNPEYRQARRYLQAHLEEKPAVSEKKKSFFYGCLITYCALSLMQNGVVVMHDAYNTL
                     SSVPQTIERNSRVFFSPDASTLTAPVQRALSTLTLSAPISQLTGLIDPSSNTASLYWT
                     IDLKNNSNMEREAQFELKLPRGASVSRATLWINGKPQVAAFNTTGKVQAAYEYIVVRH
                     RDPLLVTWKDAQTIIVKAAPVPANGTPMRIRLGLTAPLACDDRGIPVLTFPGLENANF
                     SMPESSLVHIESTKPLSAEGKLGRVVRGGFNSYVFKAHSQGQELSRLKLQSLAPEEAN
                     TFAVRATHAPKGTYIVATATGDSEGEAMVLKRVTTLPDIPVLKDEDAAHRVSTLWAAQ
                     EVQRLVGEGRMAEAEELASTFRIVSPVSGAVVLETENDYQTQGLHREQYRQTTYSGDL
                     SQNNLQVGSMPTLQGATNGTLAPQGLSLGIGSIASPSYPQIQ"
BASE COUNT          624 a          660 c          538 g          449 t
ORIGIN      
        1 tgtttttcta aaatccccca agacaagccc ctggcgccga actccgttac caccaagacg
       61 acagtcacgg caactctcag ggactccaac ccaatggtcc tagcgaccta tcagatggca
      121 ggctatctga ttttagaaaa actcctgttt ttctaaaatc ccccaagaca agcccctggc
      181 gccgaactcc gttaccacca tggcgactgt ctcagcaact tccaggtact acaggcctat
      241 ggtccacgcg acccatcaga tggcagacta tctgatttta gaaaaactcc tgtttttcta
      301 aaatcctctg tcccacgcgc ctgcccaatg ccataagctt atacaactta agggcaggct
      361 agcaaaccct tcgagaaaaa tcgaagtttt tctcgaatca ctcaaacacc ctgccagaat
      421 cccaacatta gacatcgctg caacgacgca gaaataatct cgcaagatga actcttggca
      481 agattccaac ttggtgttat attgcaactt gcggagcttt tttccgcatt tgtccaggaa
      541 tccctttact aagcaacaat cacatagcac gagccacgag atttatctcg gctttggcat
      601 ggccggcaac tttctaggtt cgccgggccg gtcgtccgac tgagcccgtc ccgcctatcg
      661 aaagccggcc gggtagttcg cctcacttcc agaggtggac tatttgaaac tggcgcgcct
      721 gccaaaaccg gcgcaggatt aagcgcgacc agtgcatgct gaatggatgc ggctacggca
      781 ttgaaggagc aaccatgaat actaagacca gcacagacaa ctcatgccag aacgagccta
      841 ttgagccagc cccgacagtg ggtcagaaca accccgtcaa actcaaggat aactggtgca
      901 gccttgagaa tacttataga aaagatgatc aaaatcctga gtatcgacag gcaagacgat
      961 accttcaggc gcacctggag gaaaaaccag ccgtaagcga gaagaagaaa agcttcttct
     1021 acggctgcct gattacctac tgcgcactct ccctcatgca aaacggcgta gttgtaatgc
     1081 acgacgccta caataccttg agcagcgtgc cgcaaacaat agaaagaaac agccgcgtct
     1141 ttttctcgcc tgatgcttcc accctcacgg ccccagtgca gagagcattg agcaccctca
     1201 ccctcagtgc tcccatctcc cagctgacag ggcttattga cccgagcagc aatacagcca
     1261 gcctctattg gaccattgat ttgaagaaca attccaatat ggaaagagaa gcccagtttg
     1321 aattgaaact acccaggggc gccagcgtct cacgcgccac gctctggata aacggcaaac
     1381 cgcaggtagc ggcatttaac accaccggca aggtgcaggc agcttatgaa tatattgtcg
     1441 tcagacaccg cgacccactg ctcgtcacat ggaaagacgc tcagaccata attgtaaaag
     1501 ccgcaccggt gccggcaaac ggcacaccca tgcggattcg cctgggactg acggcacctt
     1561 tagcctgcga tgatcgggga ataccggttc ttacttttcc cggtctggaa aacgcaaact
     1621 tctccatgcc tgagtcatcg ctggtgcaca tagaatccac aaaaccgctg tcggcagaag
     1681 gaaaattagg acgcgtggta agaggcggct tcaacagtta tgtcttcaaa gcacacagcc
     1741 agggtcagga gctgagtcga ttgaaactgc agagtttggc gccggaagaa gcaaatacct
     1801 ttgccgtgag agccacccat gcccccaagg gcacctatat tgttgccacc gccaccggtg
     1861 acagcgaagg cgaagccatg gtactgaaac gcgtcactac cctaccagac attccagttc
     1921 tcaaagatga ggacgcagcc cacagagtct ccacgctttg ggctgcccag gaagtgcaaa
     1981 ggctggtagg cgagggaaga atggcggaag cggaagaact ggcttccacc ttccgcatag
     2041 tttcaccggt cagcggtgct gtggtgcttg aaactgaaaa cgactaccag actcaagggc
     2101 tgcacagaga gcagtacagg caaacaacct atagcggcga cttgagccaa aacaacttgc
     2161 aggtcggtag catgcccacc ctgcagggag ccacaaacgg caccctggca ccacaaggct
     2221 tgtctctcgg tattggctcc atagcaagcc cgagctatcc acagatccag g
//
LOCUS       DAOQNZ010000644         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29680_length_2266_cov_3.939394, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000644 DAOQNZ010000000
VERSION     DAOQNZ010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29680_length_2266_cov_3.939394"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1209)
                     /gene="ltaE"
                     /locus_tag="PKW73_11620"
     CDS             complement(175..1209)
                     /gene="ltaE"
                     /locus_tag="PKW73_11620"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: protein motif:HMM:NF007825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNA73983.1"
                     /translation="MKAKPIDLRSDTVTLPPEAMLAAMIKAPLGDDVYGEDPTINALE
                     QRLAQMTGKDAALFVASGTMGNLVASLSHCGRGDEIILGDRSHIARWEQGGASTLGGI
                     SLRTAPNQADGTIALNDLRASVNEDDPHRTVTRLICLENTWMGQPLSVEYMHAVSELA
                     LNRNLSLHLDGARLFNAATYYKTDISTFTSLVDSVQICFSKGLSAPAGSIVCGSHDFI
                     SRARRWRKALGGGMRQAGVLAAAALYALDNMVERLQEDHENASLLSNILSEIKGIKQT
                     TAVKTNMVFFEPTAESGMDRQDLLGKMKDAGILAGIESNLGIRAVTHYGISKADIEEV
                     GKRMKAVYQA"
     gene            complement(1220..>2266)
                     /locus_tag="PKW73_11625"
     CDS             complement(1220..>2266)
                     /locus_tag="PKW73_11625"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNA73984.1"
                     /translation="YLGELSKRSIDAVSSVGELLSSRVLAEYATARGMQVTWLDARKL
                     VITDDNFGKANPDWKEIEKRAAQIAELSKKGSTVITQGFIGSTADGVTTTLGRGGSDY
                     SASILGVAAGAKCIEIWTDVDGMMTADPRIVKNALPISEASFQEASELAYFGAKVLHP
                     LTIKPAVEKDIPVKVLNTMRPESEGTLIQSKPSAVFGKDGKARSVCAIASKKNITALF
                     ISSPRMLMAHGFLAKVFNVFDKNKTPIDLIATSEVSVSLTVDSTENLEAIREDLKELG
                     ELQVLHNTAIVTVVGRQFRERSGIAGEVFSALKDVNILVISGGASEINLSFLVMAEDS
                     DRAVQQLHAHFFDD"
BASE COUNT          425 a          565 c          702 g          574 t
ORIGIN      
        1 gtgacggcct tccgggtgga gagtattaac ctgcagggca tagagaagcg caattttatg
       61 attaccactt cttccaataa cttcaatcta ttggggatgg attttcttgg ggacaggcgc
      121 ttcgttattg ataacgagaa gtctttgatt cgcttctttc gctaagtgcg caggctaagc
      181 ctggtaaacg gctttcatgc gcttgccgac ttcttcaata tcggctttgc tgatgccgta
      241 gtgggtgacg gctctgattc ccaaattgct ctctataccg gcaaggatgc cggcgtcttt
      301 cattttgccg agtaaatctt gcctgtccat gccgctttca gccgttggtt cgaagaatac
      361 catattggtt ttgacggctg tagtttgttt gatgcctttg atttcactga gtatgttcga
      421 gagcaggctg gcgttttcat ggtcttcctg gaggcgttct accatgttgt ccagggcata
      481 gagggctgct gccgccagca cgccggcctg tctcatgccg ccgccaaggg ctttgcgcca
      541 gcgccgggcg cggctgatga agtcgtggct gccgcagaca atggagccgg ccggggcgga
      601 gagccctttt gaaaagcaga tttgcaccga gtctaccagg ctggtaaagg tggaaatgtc
      661 ggtcttgtag taggtggcgg cgttgaatag ccgggcgcca tccaggtgca agctcaggtt
      721 gcggttcagg gccagttcgc tcactgcatg catgtattcg acagaaaggg gctgtcccat
      781 ccaggtgttc tcaaggcaga tgaggcgggt gacggtgcgg tgcggatcat cttcgtttac
      841 cgaggctctc aggtcattga gagcgattgt gccgtccgct tggttgggag cggtgcgcaa
      901 gctgatgccg cccagagtgg aggcaccgcc ctgttcccag cgggcaatat gacttctgtc
      961 tccaaggatg atttcatcgc cgcgaccaca atgactgagg ctcgccacca ggttacccat
     1021 ggtgccgctg gcaacgaaga gggcggcatc tttgccggtc atttgcgcca gtcgttgttc
     1081 cagggcattg atggtggggt cttcgccgta gacgtcgtcg cccagggggg ctttgatcat
     1141 ggcggccagc atggcttcgg gcggcagcgt gacagtgtcg ctgcgcagat ctatgggctt
     1201 ggctttcatg atttagacct tagtcgtcga agaagtgagc gtgcagttgc tggacggctc
     1261 ggtccgagtc ttcggccata accaggaagc tcaggtttat ctcggaggcg ccgccggaga
     1321 tgaccagaat gttcacgtct ttcagagctg agaagacttc gccggcaatg ccgctgcgct
     1381 ctctgaattg tctgcccacc acggtgacga tggctgtgtt gtgcaaaact tgcaactcgc
     1441 ccagttcttt caggtcttcc cgaattgctt ccaggttttc ggtggagtct acggtgagcg
     1501 acaccgatac ttccgaggtg gcaatcaagt cgatgggggt tttgttcttg tcgaagacat
     1561 tgaaaacttt ggccaggaaa ccgtgggcca tgagcatgcg cggagaactg atgaagagtg
     1621 ccgtgatgtt tttcttggag gcgatggcgc agacacttct ggccttgccg tctttgccga
     1681 agacggcgga gggcttgctc tgaatgaggg tgccttccga ctcggggcgc atggtgttga
     1741 gcactttgac cggaatatct ttctcgacgg ccggtttgat ggtgagcggg tgcaagactt
     1801 tggcaccgaa gtaagccagt tcggaagctt cctggaagga ggcctctgag ataggcaggg
     1861 catttttgac gatacgcgga tcggcggtca tcatgccgtc tacgtcggtc caaatttcaa
     1921 tgcacttggc tccggctgct acgccgagaa tggaagctga gtaatcggaa ccgcctctgc
     1981 cgagggtggt agtgacacca tcggcggtgg agccgataaa cccttgcgtg atcactgtgg
     2041 aacctttttt gctcagttcg gcaatttgag cggcgcgttt ttcaatttct ttccagtccg
     2101 gattggcttt gccgaagttg tcgtcggtga ttaccagttt acgcgcatcc agccaggtaa
     2161 cttgcatgcc tctggcggtg gcgtattcgg ccagcacccg ggacgatagc agttcgccca
     2221 cggaggagac ggcgtcgata gagcgcttcg atagttcgcc cagata
//
LOCUS       DAOQNZ010000645         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29692_length_2266_cov_3.137042, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000645 DAOQNZ010000000
VERSION     DAOQNZ010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29692_length_2266_cov_3.137042"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /gene="recA"
                     /locus_tag="PKW73_11630"
     CDS             complement(<1..807)
                     /gene="recA"
                     /locus_tag="PKW73_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNA73985.1"
                     /translation="MAVVTKEKTQVEKNTEKPSKASADKASKNEPTPSQERLKALGLA
                     LDSIKKQYGDGSIMKLGDSRHTSVEVVPTGALSLDVALGIGGLPRGRIIEIYGPESSG
                     KTTLAQHVAAEVQKLGGIAAIVDAEHAMDPEYARALGVNVDELLISQPDTGEQALEIT
                     EQLVRSGAVDLVIVDSVAALVPKAEIEGEMGDSLPGLQARLMSQALRKLTAVVAKTHT
                     IVIFINQLRQKIGVMYGNPETTTGGNALKFYASIRLDIRKIETLKKDGVEV"
     gene            1260..1952
                     /locus_tag="PKW73_11635"
     CDS             1260..1952
                     /locus_tag="PKW73_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNA73986.1"
                     /translation="MKGAQRLFSLRQSEIFESFNPVELGRLLGILEELELPKHHLIFS
                     PGTPCDAIYFIEKGRVRVTRLSTEGKTVILALLGPGDMIGEAAWETDEHDSYAETLED
                     SRIYQIGREAFLNFVRENPEFGLRFIGILGDRLKQAQARIEDLVFRQVPSRVARLLVT
                     LAETHGKVTPNGVRVEFPLTHQEIADMVGSSRVTVTQILNRFRDSHWIDIESKRVTIH
                     NMNALEELVRHL"
     gene            1970..>2266
                     /locus_tag="PKW73_11640"
     CDS             1970..>2266
                     /locus_tag="PKW73_11640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA73987.1"
                     /translation="MTDTPEQVQLLHGWLNAPHVAERWDGPLTLAAVQESYFKDEVEK
                     CERFLVYLDGQPLAYIQSYIACLCSDGWWEDITDPGTVGIDFFIGEAGKLNHGLG"
BASE COUNT          507 a          570 c          621 g          568 t
ORIGIN      
        1 gacttcgacg ccgtctttct taagggtctc tattttacgg atatccaatc tgatggaggc
       61 gtagaacttg agcgcattac caccggttgt ggtttcgggg ttgccgtaca tgacgccgat
      121 cttctgtctg agctggttga tgaagatgac gatggtgtgg gtcttagcta ccacggccgt
      181 gagtttgcgc agggcctggc tcatcaggcg ggcttgcaga ccaggcaggc tgtcgcccat
      241 ttcgccttca atctcggctt tcggcaccag agccgctacg gagtcgacga tgaccaggtc
      301 tacagctccg gagcgcacca gttgctcggt gatctcgagg gcttgttcgc cggtgtcggg
      361 ctgcgagata agcaactcat cgacgtttac gcccagggcc cgagcgtatt cgggatccat
      421 ggcgtgttcg gcgtcgacta tggcggcgat gccgcccagt ttttgtactt ctgctgccac
      481 gtgctgagcc agggtggttt taccggaaga ttcaggtccg tagatttcga tgattcgacc
      541 tctcggcaga ccgccgatac ccagggccac atcgagggaa agagcaccgg tgggtactac
      601 ttctaccgag gtgtgacggg aatcgcccaa cttcatgatg gagccgtcgc cgtattgctt
      661 cttgatggaa tcaagagcca gtccgagggc cttcagcctt tcctgggaag gcgtaggctc
      721 atttttgctt gctttgtcgg cagaggcttt gcttggcttt tccgtgttct tttccacttg
      781 cgttttctct tttgtgacta ccgccatatt aattctcctc aaatagataa gctaaagctc
      841 aagctatcgc caaaccatac aaatagcgca agcctttcaa aaaggcatat ttaaactgtg
      901 ctgataaatc tgcacacccg gcaaattata acggctttgc ccctcagctc gaattaaaac
      961 tcagcaggaa acccgcttgc tgtctagtgt atatgcgatc ggtgcagttt gctgggcgga
     1021 tttatggcat agatcctggt gggatccttg cttttgccat ccttgctaga tagttctttc
     1081 gaggggaacc tcttgggaag ttataaaaag ggtggcgggg gtataaaaag gttgttaccc
     1141 aacaaaaaac aaccgaaggg taatacagat aacgttatct cacgcggctt agttcaataa
     1201 agccggagaa aacttgtcac gtttaatatt tcgcgggata agcataagag gtcagaccta
     1261 tgaaaggtgc tcagagactg ttcagtttga gacagtcgga gattttcgaa tccttcaatc
     1321 ccgtggaact ggggcggctt ttaggcatct tagaagagct ggagctgccc aagcaccatc
     1381 tgattttctc tcccggcacc ccctgtgacg ccatctactt tatagagaag gggcgggtgc
     1441 gggtgaccag gctttccact gaaggcaaaa ccgtaattct ggctctgctc gggccgggcg
     1501 acatgatagg cgaagcggcc tgggagaccg acgagcacga tagttatgct gaaacactgg
     1561 aagacagccg catttaccag attggccggg aagccttcct caatttcgtg cgggagaatc
     1621 ctgaatttgg tttgcgcttt ataggaattc tcggcgatag gctcaagcaa gcccaggcgc
     1681 gtatcgaaga tctggttttc aggcaagttc ccagccgcgt cgcccgtttg ctggtgacac
     1741 tggccgaaac acacggcaag gttactccca acggcgtgcg agtggaattt cctctcaccc
     1801 atcaagaaat cgccgatatg gttggctcca gccgggttac cgtcactcag atcttgaata
     1861 gattccgtga cagccactgg atcgatatcg agtccaagcg agtcaccatc cacaatatga
     1921 atgccctgga agaactggtg cgacatctct gaactttcgc tttgagaaat tgaccgacac
     1981 cccggagcaa gtgcagcttc tgcatggttg gttgaacgcg ccccatgtgg ctgagcgctg
     2041 ggacggacct ttaaccctgg cggcagtgca ggaatcttat ttcaaagacg aggtagagaa
     2101 gtgcgagcgc ttcctggtct atcttgatgg ccagccattg gcctatattc aaagttatat
     2161 agcctgcctt tgctcagacg gctggtggga agacattacc gatccaggca ctgtaggcat
     2221 cgacttcttt atcggtgagg ccggcaagct caatcacggt cttggt
//
LOCUS       DAOQNZ010000646         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29710_length_2265_cov_4.191403, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000646 DAOQNZ010000000
VERSION     DAOQNZ010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29710_length_2265_cov_4.191403"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="PKW73_11645"
     CDS             <1..1605
                     /locus_tag="PKW73_11645"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA73988.1"
                     /translation="KVKEMKSKEETKVAIIGAGVNGLTCAAYLAKAGFQVTVFEKNQQ
                     AGGLCVNQTPFKNSAIKVSAVASYYGMLREEVAEELGLYERGLTPYLTDPIEIVLLKD
                     RQYVYYPREGSADMQAEETTEEDKKGWVDFWTDIQRAAQIVYPHYLKPITAKEYLEAL
                     KQAGLDKIAETIYTGSLFDLADKYFQSPALKAVAATCTPGFANLVGSVFGCIHHGTAS
                     TLGNFGAWGQVKGGMGKISEAILQRATECGAKLKTGLGIKSINIEGESVGTIVSEDGQ
                     EHRFDLVIMSGDPYTLFEKLIDCELVPSGIRAYLAANRPRVSAAKLHYLLKKQVNFQT
                     LERIGHNHKGVLVIAPTPEAVKQASRAVPEGNLPEELMLTMAYPTLEDESIYGEEKNK
                     DRHVLTVDVHYLPAQLKGKNGLRPWQKEDDETLSRAVLNTIEKYAPGFIEQVEESYIV
                     SPGGLKSLFNNESLSCWHMPMSQDFLFENRALPTLPHYHTPFANLYLCGSSTFPGGNV
                     TGANGHNLAKLLINAYSPALTPTSAK"
     gene            1622..>2265
                     /locus_tag="PKW73_11650"
     CDS             1622..>2265
                     /locus_tag="PKW73_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73989.1"
                     /translation="MNLTEHDMKEQDGITYIEEMPVKTVQVFQATKEHLPLAGEIATM
                     YEAALGKGGLRAQGMEPYPDSDLFSEEGVKATLEAGERTLAIARIKDEGGDCLAGAMV
                     MDRLSPHHVEFNSMAVRLDKRGRHVGTAIVEGLKQIVEETEFTVNATELVTHSLASQA
                     AHFHNGYKNICGFSFCHYPRVFFKNHPESVLWVMLLQGKLIPLMRRFRSILGRAL"
BASE COUNT          610 a          661 c          578 g          416 t
ORIGIN      
        1 aaagtgaaag aaatgaaaag caaagaagaa acgaaagtag caattatagg ggccggcgtg
       61 aacggactga cctgcgccgc ctacctggcc aaggccggct ttcaggtcac cgtctttgaa
      121 aagaatcaac aggcaggcgg gctctgcgtc aaccagaccc ccttcaaaaa tagcgccatc
      181 aaggtttcgg cggtggcatc ctattacggc atgctgcgag aagaagtagc ggaagaactg
      241 gggctctatg agcgggggct gacgccttat ttgaccgacc ccatcgaaat agttttgctc
      301 aaagaccggc aatatgtcta ctacccgcgc gaaggctccg ccgatatgca ggcggaggaa
      361 accacggaag aagacaagaa aggctgggta gacttctgga ccgacattca aagagcggct
      421 caaatcgtct atccccacta tctaaagccc ataaccgcga aagaatatct ggaagcgctc
      481 aagcaagccg gcctcgataa aattgccgag accatctaca caggcagcct ctttgacctt
      541 gccgacaagt acttccaatc accggcacta aaagcagtgg ccgccacctg cacccccggc
      601 ttcgccaatc tggtcggttc cgtcttcggc tgcatccacc acggcaccgc cagcaccctg
      661 ggcaatttcg gcgcctgggg tcaggtaaag ggtggcatgg gcaaaataag cgaagcgatc
      721 ctgcaacggg ccaccgaatg cggtgccaag ctcaagaccg gtctgggtat aaaatccatc
      781 aatatagaag gcgagagcgt cggcaccata gtcagtgaag atggtcagga gcaccgcttc
      841 gatctggtca taatgagcgg cgatccctat accctctttg aaaaactaat cgactgcgaa
      901 ctggtgccct ccggcataag ggcctatctg gccgccaatc gcccccgggt ttcggcagcc
      961 aaactgcatt acctgctcaa gaagcaagtc aacttccaga cccttgagcg catcggtcac
     1021 aatcacaaag gcgtgctggt catagccccc acgccggaag ccgtcaagca agcctctaga
     1081 gccgtgccgg aaggcaactt accggaagag ctgatgctca ccatggctta tcccaccctg
     1141 gaagatgaaa gcatctatgg ggaagagaag aacaaagacc gccatgttct aaccgtagac
     1201 gtgcactacc tgcccgccca gttgaagggc aaaaacggtc tgcgcccctg gcagaaagaa
     1261 gacgatgaga cgctcagccg ggcggtcctc aatacaattg aaaaatacgc accgggcttt
     1321 atagagcagg tagaagaaag ctacattgtt tccccggggg gactgaagtc gcttttcaac
     1381 aatgagtctc tcagttgctg gcatatgccc atgtcccagg acttcctctt tgaaaatcgc
     1441 gccctgccta ccctgcctca ctatcacaca cctttcgcca atctctatct ttgcggttcc
     1501 agcaccttcc ccggcggcaa cgtcaccggt gccaacgggc acaacctggc caagcttcta
     1561 atcaacgcct atagccccgc tttaacccca accagcgcta aataaactaa agagaagcaa
     1621 aatgaacctg accgaacacg acatgaaaga gcaagacggt attacctaca ttgaagaaat
     1681 gccggtaaag accgtgcaag tcttccaggc cacaaaagag catctgcctc ttgccgggga
     1741 gattgccacc atgtacgaag ccgctcttgg caaaggcggt ctgcgggccc aaggcatgga
     1801 gccttatccc gacagcgatc tcttcagcga agagggagtg aaagccaccc tggaagcagg
     1861 ggagcgcact ttagccatcg ccagaatcaa agacgaaggg ggcgattgcc tggccggggc
     1921 catggtcatg gataggctca gcccccacca tgtggaattc aactcaatgg ctgtacgcct
     1981 cgacaagaga gggcgccacg taggcacggc catagtggaa ggtctcaagc aaatagtgga
     2041 agagaccgaa ttcaccgtca acgccaccga acttgttacc catagcctgg cctcccaggc
     2101 cgcccacttt cataacggct acaaaaacat ctgcggcttc agcttctgcc actatccaag
     2161 agtcttcttc aagaaccatc ccgagtcggt cttgtgggtg atgcttctgc agggaaaact
     2221 gattcccctc atgagacgct tccgaagcat actcggccgt gcact
//
LOCUS       DAOQNZ010000647         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29774_length_2263_cov_3.495018, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000647 DAOQNZ010000000
VERSION     DAOQNZ010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29774_length_2263_cov_3.495018"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PKW73_11655"
     CDS             complement(<1..909)
                     /locus_tag="PKW73_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA73990.1"
                     /translation="MPITSAKLRLLSLLSLSTGLLSGSLLWPLESPAQSKQAPSKPAQ
                     SKPAQSKPTAVPNSYPNYTQGASRYQEKRYNEAIACFSEAINKGEAGAAAWLYIAHSY
                     LAMGNRQEAVLTYQKIAASFPTSQEAQVARTYLQKLGAPGGAPATAAQSREEDRAAAN
                     TGSDIKNRIEVVRPVVGHPEVSEQMIQSIKRCVAGLPKGIRKILNRGQIKIILTPTLI
                     DKEPSLGYREGRGYEGNTYKSCPGMFRRDSIYICERTVNESTDEVQSARPINEIEGTF
                     YHEVGHALDACLEQYSESDEYRHAYYL"
     gene            1054..1926
                     /locus_tag="PKW73_11660"
     CDS             1054..1926
                     /locus_tag="PKW73_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73991.1"
                     /translation="MPLAVTLPDLEQAHLRCYFVFDVADSIKLSSLKSVKGQNFVPEP
                     LSFTDVPLPGYIQFSVAPLAVALNAVQTPFGELDCRVKVYEYGTVSIRFSRPFQGAFQ
                     AFRSLAMKMRFSKELMTCATQVLEEVLSELKPALSKPHEEAQQKDLLEDYYVAEVERF
                     SSPLTATELMQEHRAAIASLVSLEEKPLSAMQVDEALKEAYSYCEHELAVLTWDQAFI
                     YDDQAGPPPSTASLNSLIRSLWNCALTTGFSIGISMRFIASRLSAYAPASSRVLPAGD
                     WWRSGRRSACGTCL"
     gene            1884..2189
                     /locus_tag="PKW73_11665"
     CDS             1884..2189
                     /locus_tag="PKW73_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73992.1"
                     /translation="MAERQAERLRHLLVDVRELSDRATNAIKIIGCAYYARLYGGISQ
                     RLSLAQWQAQVESKLDSVGELYRFANDQAELARSEFLEIIVIALIALEVLIGIFGRH"
BASE COUNT          485 a          566 c          607 g          605 t
ORIGIN      
        1 caggtagtag gcatggcggt attcatctga ttccgagtac tgttcgaggc aggcatcgag
       61 cgcatggccg acctcgtggt agaaagtgcc ttcgatttcg ttaatcggac gcgcactttg
      121 tacctcgtcg gtgctctcgt tcactgtgcg ttcgcaaatg tagatagaat cacgtctgaa
      181 catacccgga caacttttgt aggtattgcc ttcataacct ctgccttccc gataacccag
      241 agatggctct ttatctataa gggtgggcgt gagaatgatc tttatctgac cccggttgag
      301 aattttcctg atgcctttgg gcagaccagc cacacaccgc ttgatggact gaatcatctg
      361 ttcactcact tccgggtgtc ctactaccgg ccgaaccact tctatgcggt tctttatatc
      421 gctcccggta ttggcagcag ccctgtcctc ttccctggac tgagcggcag tcgctggagc
      481 accgcccggc gcccccagct tctgcaagta agtgcgagcg acctgggctt cctgtgatgt
      541 gggaaaggag gcggcaattt tctggtaggt caggacggct tcctgcctgt tgcccatagc
      601 cagataagag tgcgctatgt agagccaggc cgctgctccc gcttccccct tattaatggc
      661 ttcgctgaag caggcgatgg cttcgttgta ccttttctct tgatagcggc tggcgccctg
      721 ggtgtaattg ggatagctgt tcggaaccgc tgtcggcttg gactgtgccg gcttggactg
      781 tgccggctta gatggggcct gcttcgactg agccggactt tcaagcggcc acagtaaact
      841 gcctgaaagg agcccggtag agaggctcaa aagcgaaagc agcctcagtt tggcgctggt
      901 gattggcaaa aatggtctga aaagtgacat agctaattta tagcatcacc gaaatttccc
      961 cgccagaaaa agatggtgtt gactacttgc caattgcttt aaactagtcg agtctaaaat
     1021 cgtcagtcag acaaaatcat gcggaagaag taaatgcctt tagccgtcac cctacccgac
     1081 ctggaacaag cgcacctgcg ttgttatttt gtctttgatg tggccgattc catcaagctc
     1141 tcttcgctca aaagtgtgaa ggggcaaaat ttcgtaccgg aaccattgag ttttaccgat
     1201 gtgcctctgc ctggttacat tcagttttcc gtggcaccgc ttgcggtagc actaaatgcg
     1261 gtgcagaccc ccttcggcga gcttgactgc cgggttaaag tctatgaata cggcaccgtc
     1321 agcatccgct tctccagacc ctttcagggt gcctttcaag cgtttcgcag tctcgctatg
     1381 aagatgcgct tttcgaaaga acttatgact tgtgccactc aggtgcttga agaagtgctt
     1441 tcagagctga agccggccct cagtaagccc cacgaagagg cgcaacagaa agacttgctg
     1501 gaagactatt atgtggctga ggtggagcgc tttagttcgc ccctgacggc gaccgagctt
     1561 atgcaagagc accgggctgc tattgcttcg ctggtttccc tggaagagaa accactttca
     1621 gccatgcagg ttgatgaagc tttgaaggaa gcctatagct attgtgagca tgagctggca
     1681 gtattaacct gggaccaggc ctttatctat gatgaccagg cggggccgcc gccgtcaaca
     1741 gcatcattga attcgctaat acgcagcttg tggaattgcg cacttacgac aggcttctcg
     1801 ataggcatct cgatgagatt tatcgcgtca agactgagcg cttacgcacc agcctcttcc
     1861 agagttttac cagcgggcga ctggtggcgg agcggcaggc ggagcgcttg cggcacttgc
     1921 ttgtagatgt aagggaactt tccgacagag ctaccaatgc cataaaaata atcggttgtg
     1981 cctattatgc caggctctac ggcggtatct ctcagcgttt gagtctggct cagtggcagg
     2041 cgcaagtgga aagcaagctt gactcggtcg gtgaactcta tcgcttcgcc aacgaccagg
     2101 cggagctagc ccgttcggag ttccttgaaa ttattgtgat tgcgcttatc gccctggaag
     2161 tattgattgg aatttttgga agacattgaa tctttgtagt tttgttcatt tgtatgtttg
     2221 tttatttgta ggtttgttca tttgcctgtt tgtttgcttc gtt
//
LOCUS       DAOQNZ010000648         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29820_length_2261_cov_3.452856, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000648 DAOQNZ010000000
VERSION     DAOQNZ010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29820_length_2261_cov_3.452856"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKW73_11670"
     CDS             complement(<1..187)
                     /locus_tag="PKW73_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73993.1"
                     /translation="MTWLEAFWLAFLALVFVRWHYLGHLAELERRISEFAEEIDALKL
                     EPLSQEQSESLNEAVRLF"
     gene            complement(689..1567)
                     /locus_tag="PKW73_11675"
     CDS             complement(689..1567)
                     /locus_tag="PKW73_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73994.1"
                     /translation="MSQCKLFILSLCLLFMVGCNPQSSVDAAQPGGSQKQVVDHDAVL
                     KLEELTDANSEELLLKRNMPSFVVVYSSKESNPAQTQQLEQIVRDSHNYAGAVNFYRL
                     DQAKYPNTWSNITNGRSWSQGPCFLLFSINPVMIGTVMEQQNGIDVPVHNMSSARMSA
                     EIARFMKIKPLVTHANLDNVDPLVIKPALPTFIMAYRSRGPHHDFVQFQRFVYESQLY
                     AGRVNFVYVDLDQPDLSSKLGIALPKQDDVYYVLYNPKSKTSQTVADPALSGRQMETV
                     ISKFFGPGHEPAKVIY"
     gene            complement(2000..>2261)
                     /locus_tag="PKW73_11680"
     CDS             complement(2000..>2261)
                     /locus_tag="PKW73_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73995.1"
                     /translation="LDASGGQRESFSVARVEVLKDGIWLPVLEYLVGQDSVLKVYSLG
                     GGERVIHLDLPPAVVRGMALNDLNSNRLEYCRKFLLGRKLFC"
BASE COUNT          580 a          582 c          595 g          504 t
ORIGIN      
        1 taaacagcct taccgcctca ttgagggatt ctgactgttc ctggctgagg ggctccaatt
       61 tgagcgcatc aatttcctca gcaaactcag agattctgcg ctccagttcg gcaagatggc
      121 caaggtagtg ccatcgaaca aaaaccagcg ccagaaaggc taaccagaaa gcttcaagcc
      181 atgtcatatg atttccattg ctgatttagt tagagcttca actatttagc tgaagctgag
      241 gaaccgcacg aaactctcac cgagaaagca ctgctcactg ttccaccttg cctggcattc
      301 ggtcagggca atgccgcaat ttgatgctgc cggtgcaagg tgtaagtaaa gccagtgcgg
      361 ttcagttgaa aaacaagtgc ttgtgtctgg aaagaaagta agtaagaaac accaatgctc
      421 ttagcagata attagccgcc atagaaagga caatcacggc acattgagaa agcagtcccc
      481 gatcagttta gcgaccatgc acaagtctca caaaggctgc aaaaatcggc ctcaacaatc
      541 gacaacatcg gcccggtagc gcctattgct accttctata gtatcttcat gctcaaaaaa
      601 atggcattaa ggcaagagcc gggaacagca tgacttggca tcatgccact ccccggctcc
      661 cggcttgtgt ttgttggtgg ttgagtgatc aataaatcac cttggccggt tcgtggcccg
      721 gaccaaagaa ctttgagatc accgtttcca tctgcctccc gctcaaggca ggatcggcaa
      781 cagtctggct ggtcttgctc ttcggattgt agagcacgta gtagacatcg tcctgctttg
      841 ggagcgcgat acccagcttg ctggagaggt ccggctggtc caggtcgaca tagacgaagt
      901 tgacccgacc ggcgtagagt tgcgattcat agacgaagcg ctgaaactgc acgaaatcgt
      961 gatgcggtcc cctgctgcga taagccatga taaaggtggg cagagccggc ttgattacca
     1021 gagggtcaac gttgtcgagg ttggcgtggg taaccagagg cttgatcttc atgaagcgag
     1081 caatctctgc gctcatacga gcgctggaca tgttgtgcac gggcacatcg atgccgttct
     1141 gctgctccat caccgtaccg atcatgaccg ggttgatgct gaagagaagg aagcagggac
     1201 cctggctcca ggagcgacca ttggtgatat tgctccaggt gttggggtac ttggcctggt
     1261 cgagacggta gaagttaacc gcaccggcgt agttatgcga atcgcgcaca atctgctcca
     1321 gttgctgagt ttgagccggg ttcgactctt tgctcgaata gaccaccaca aaggaaggca
     1381 tattgcgctt gagcagaagc tcttcgctat tggcgtcggt cagctcttcc agtttcagga
     1441 cggcgtcatg atccacgacc tgtttctggc ttccacccgg ctgagcggca tccaccgagc
     1501 tctgagggtt gcagccaacc atgaaaagca gacataggga aaggataaac agcttgcatt
     1561 gagacatgga ggttcctctt ggaaaagtgc caggtttgct ggcaggtttg ggaagattaa
     1621 caggccaagg caggaagcaa aagtcgcgcc ggggcttcga ctttcaattc catacgaggg
     1681 gcacacaggg caggctcaag ccggagatac ttggttatcc gcttttgcct ttggtttgct
     1741 gatagatgcc ggttgcttgc ctgggtttaa ataccgaatt agagttttaa ttggtgaacc
     1801 cctcttttct accagggaag aaccacgaat tgcaaagggg ttactacata acatcatcta
     1861 acattagaat ctctgaaaat tcgcatcgcg ctagctcccg cggcatctca atcggcgctg
     1921 aaatcggtaa cgtccgataa aatgcaaaca attgagccgg ctcagattaa gcacagcaaa
     1981 gaaatagatc agccaggtgc tagcagaata gcttgcgacc aagcaagaat ttgcggcagt
     2041 actccagccg attgctgttc aggtcgttga gcgccatgcc ccgtaccacg gcaggcggca
     2101 gatccaggtg aatgactcgc tcgccgccac ccagactata gacctttaat accgagtcct
     2161 gaccaaccag gtactcgagt acaggcaacc agattccgtc cttaagaact tccacccgcg
     2221 ccaccgagaa agattcccgc tgcccgccgg aagcatccag a
//
LOCUS       DAOQNZ010000649         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29890_length_2258_cov_3.003631, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000649 DAOQNZ010000000
VERSION     DAOQNZ010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29890_length_2258_cov_3.003631"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKW73_11685"
     CDS             complement(<1..267)
                     /locus_tag="PKW73_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73996.1"
                     /translation="MDAPSTPGAGTLFLIGGKANETLNRFVELSGGKQALIVILPHAS
                     SIAAEVSAELSKTLLDKGAGQCCIIMPGEDRSLPEGTTSVYLMGG"
     gene            complement(573..2117)
                     /locus_tag="PKW73_11690"
     CDS             complement(573..2117)
                     /locus_tag="PKW73_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA73997.1"
                     /translation="MAGFSELLSPVHSPEKKKILEAIATRGPRLVPVDVAASTGLALP
                     LVVSELNNIAAETNAHLEVTESGNIAYKFQGNLQQAYLANASRQILLSAWRVFANASI
                     IALRAFCALMFFLVRISFGVMLIVAAVAVVVLVVVAIVAALKGLSGGDGDSDGGFNLD
                     FGNIFDFGWHDGYYHRPFYLYWAFDWLWDWFFFWRYVLPDPYYPRGGYGNSGSYNAQT
                     DYLNKKAGEGEKKGNFLNNVFSFLFGDGNPNANFEELKWQTVTRIIELNQGVVTAEQL
                     APYLGENPAEEDWMIPVLQRYNGSPEVSENGNIIYVFTAFQNQPGGNSIPMGSLDAAK
                     NQTRPVDELAGLFQNHVKRQSTTKKSEAQLRFIDKLLPEKEWEFLTIDGGAVTGIILF
                     ALFITFGGAALLTTTASLPIMSHFTALIYLIMGYGGLFFLIPAIRYAIYKMINEGIEK
                     RNQAKQVYAANLANPSPELSKKLSEATTVRVAGLPKKPDKTVYSTEQDVYEQGYSEPD
                     DFSPPS"
BASE COUNT          535 a          552 c          632 g          539 t
ORIGIN      
        1 accgcccatg aggtagacgg acgtggtgcc ttccggcaaa gagcggtctt cgccgggcat
       61 gatgatgcag cactgtccgg cgcccttgtc gaggagggtt ttggacagtt cagccgatac
      121 ttcggccgcg atgctgctgg cgtgtggaag aatgacaatc agggcttgtt tgccgccgct
      181 cagctcgaca aaacgattga gtgtctcgtt tgctttgccg ccgatcagga aaagagtgcc
      241 ggcacccggg gtagatggtg catccatgat gagttccctc tgcaaaaggt ggttatgatg
      301 tgcaagaaca gatcggcgcc aaagctgacc aatcagggaa agattgagaa ctgttcttgt
      361 tatttgggga gctactggct aaaactaaac tagggaaggt ggagaagatt caaagctcac
      421 cttatctttc tcctggcagg aagagcaatt gcttctttgc ctttgaggct tcaatcgctc
      481 taattatagc tttgctggat tgcctgagcc gcaggtaaga ccgtgatcgg ctagcctggc
      541 tagcgctaga gatggtcttt cttagcggca cttcatgacg gcggcgaaaa atcgtcaggc
      601 tcgctgtagc cctgttcata aacgtcttgt tcagtactgt agacggtttt gtccggtttc
      661 ttcggtagac cggctacgcg aaccgtggtt gcttccgaga gcttcttgct gagctcggga
      721 gaaggattgg ctagattggc cgcatagact tgtttagcct ggttgcgctt ttctatgcct
      781 tcattgatca tcttgtaaat ggcgtagcgg atggccggaa tcaggaagaa aagaccgccg
      841 tatcccataa tcagatagat aagggcggtg aaatgcgaca ttatcggcag gctggcagtg
      901 gtggtcaaaa gtgccgcgcc gccgaaagta atgaagagag caaagagtat gatgcctgtg
      961 acggcgccac catcaatggt gagaaactcc cactcttttt ctggcagaag cttgtcgatg
     1021 aatctgagct gggcttcact tttcttggtg gtagactgcc ttttgacatg gttttgaaag
     1081 agaccggcca gttcatccac cggtcttgtc tggtttttgg ccgcgtctaa tgagcccatg
     1141 gggatggaat tgccccccgg ttggttttga aaggcagtga aaacataaat gatattgccg
     1201 ttttccgaaa cttccgggct gccgttgtag cgctgcagca caggaatcat ccagtcttct
     1261 tcggccgggt tttcccccag gtagggagcc aactgttcgg ccgtgaccac gccctggttc
     1321 agttcgatta tgcgagtgac agtctgccac ttgagctctt caaaattggc attgggattg
     1381 ccgtcgccga aaaggaagga aaagacgttg ttgagaaagt tgcctttctt ttcgccttcg
     1441 ccggcttttt tgttgagata gtcggtctgg gcattataag aaccactgtt gccgtatccg
     1501 ccccggggat aataggggtc gggcagcaca tagcgccaga aaaagaacca atcccagagc
     1561 caatcaaagg cccagtacag ataaaagggg cgatgatagt aaccgtcatg ccagccgaaa
     1621 tcgaagatat tgccgaagtc caggttgaag ccgccgtctg aatctccgtc gccgccgctc
     1681 agtcctttca gggccgccac gattgccact accaccagta ctaccacggc taccgcggca
     1741 acgatcaaca tgacgccgaa ggaaatgcgc accaggaaga acattaaggc gcagaaggct
     1801 ctcagggcga taatggaagc gttggcaaag acacgccagg cggaaagtaa gatttgacgg
     1861 gaagcattag ccagataagc ctgctgtaaa ttgccctgaa acttgtaggc gatattgccg
     1921 gattccgtca cttccaggtg agcattggtt tcggcggcaa tattgttcag ttcgctcacc
     1981 accagtggta aggcgagacc ggtgctggct gctacgtcca ccggcacaag gcgtgggccc
     2041 ctggtggcga ttgcttccag gatttttttc ttttccggcg aatgcaccgg actgagaagc
     2101 tcactaaagc cagccaaatt gaaaactcca gagactacct agtagctatt ctgactgaaa
     2161 aatagccgtt tcttcaaaat catcatctgg tctaaataaa tggcgggacc gacgggactc
     2221 gaacccgcga cctcctacgt gacaggcagg cgctctaa
//
LOCUS       DAOQNZ010000650         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29929_length_2256_cov_4.211268, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000650 DAOQNZ010000000
VERSION     DAOQNZ010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29929_length_2256_cov_4.211268"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PKW73_11695"
     CDS             complement(<1..1037)
                     /locus_tag="PKW73_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA73998.1"
                     /translation="MSLRETRRVICPHDCPDTCSMSVTVEDGRVVSVRGDESHPFTRG
                     FLCVKTNHYLERLYSPLRLLVPQKRVGRKGEGRFAPITWEEAIDTIAERFQSIASQYG
                     PEAVLPYSYAGNMGKLAFGSMDRRFFHYFGASRLLRTICATAATEGYLHTMGDRLGTD
                     PESLPESKLIIAWGSNFVSSNVHLVPFVNEARKRGAVLVTVDPHKNRTAEQSDLFIQP
                     LPGTDAALALGMMHVIVQEGLHDEQFIKENTIGFDALASHLAAYDPARVAAITGLSQD
                     QIVDFARLYARLKPSCIRLNYGLSRHTNGGMMVRTVSCLPALVGAWGVPGGGAMLSLS
                     GAYPLNLAALE"
     gene            1151..1522
                     /locus_tag="PKW73_11700"
     CDS             1151..1522
                     /locus_tag="PKW73_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNA73999.1"
                     /translation="MNIPKKTLSIYLIAALFLFAGVNHFTQPELFLKAMPPWLPWHLP
                     LVHISGVFEILGAIGMLVTKSRPYAAYGLVLLLIAVFPANLHMALNPNTFPTLPVALL
                     YCRLPLQLVLIAWVYRTGKAN"
     gene            complement(1524..>2256)
                     /locus_tag="PKW73_11705"
     CDS             complement(1524..>2256)
                     /locus_tag="PKW73_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74000.1"
                     /translation="QASEFPDSAEAAFIHAVALTRSSQVEAALKEVRRARNLARATGD
                     PNYFNRAVSQYEQSLDADPNNSCVRYGLSWAYYMQAYLLGEQARKQESIAKQPIMPQM
                     YRKKSHLGDNLLNGATILASALTGTKPPVGAVPHIPGALEGAPTWAIPQIKQYYQKSI
                     NQLDQVIKREPTNAWAATYRVHVNEEYDGNNEAALRQLEALKKRFPDNPAVAFFLADS
                     YARNGNFAAGAGSLGRAVQLKLQGK"
BASE COUNT          532 a          656 c          562 g          506 t
ORIGIN      
        1 ctttcaagcg cagccagatt caagggataa gcgccgctca agctgagcat tgcgccaccg
       61 cccggcacac cccaggcacc caccagagcc ggtaaacagc tcacggtgcg caccatcatg
      121 ccgccgttgg tatggcggct gaggccataa ttgaggcgaa tacaactggg tttgaggcgg
      181 gcataaaggc gagcgaagtc aactatctgg tcttgagata ggccggtaat ggccgccact
      241 ctggccgggt catatgcagc aaggtgcgag gccagcgcat caaagccaat ggtattctct
      301 ttgataaact gctcgtcgtg caaaccctcc tgcacgatca catgcatcat gcccagcgct
      361 aaagctgcat ccgtccccgg caaaggctga atgaacaaat cggattgctc agcagtgcgg
      421 ttcttatgcg ggtcaaccgt gaccaatacg gcaccacgct tgcgcgcctc attcacaaag
      481 ggcaccagat gtacattgga agatacgaaa ttggaacccc aggctataat cagcttactc
      541 tcgggcaaag actcaggatc ggtgccaagt ctgtcaccca tggtatggag atacccctcg
      601 gtggcagcgg tagcacatat ggtgcgcagc aagcggctgg cgccgaagta atggaagaaa
      661 cgtctatcca tggagccgaa agccagtttg cccatattgc ccgcataact atacggcaag
      721 acagcttccg ggccatactg agaagctatc gactgaaagc gctcggcaat ggtatctata
      781 gcctcttccc aagttatggg agcgaagcgc ccctcaccct tcctgccgac gcgcttttgc
      841 ggcacaagca gccgcaaagg gctataaagc cgctccagat agtgattggt cttgacgcac
      901 aaaaaaccgc gggtgaaagg atggctctca tctccccgca ctgaaaccac ccggccgtct
      961 tctacggtta ccgacatcga acaggtatcc ggacaatcat ggggacagat gactcgcctg
     1021 gtctctctaa ggctcactga atctcccttt tactaacttg caggttattt cacggtcacc
     1081 gggactatat aatattgaaa gcttcatcgt tttcacaggc agaagattta attacactca
     1141 gccccaacta ttgaatatcc caaagaaaac tctatcaatc tacttaattg ccgccctctt
     1201 tctgtttgcg ggagtaaatc acttcactca gccggaattg tttctcaaag ccatgccccc
     1261 ctggttgccc tggcatttac ccctggtaca catcagcggt gtctttgaaa ttctcggcgc
     1321 catcggtatg ctggtcacaa agagcagacc ttatgccgcc tacggcctgg tgctactcct
     1381 catcgccgtc tttccggcca acctgcatat ggccctcaac ccaaatacct ttccaaccct
     1441 accggtggct ctactctact gccgcctgcc tctgcaactg gttttgattg cctgggtcta
     1501 tcgaaccgga aaggcaaact aatttacttg ccctgcaatt tcaactgcac ggcccgtccc
     1561 agagatccgg ctccggcagc gaagttgccg ttgcgagcat aggagtcggc aagaaagaag
     1621 gccacggccg gattgtccgg gaaacgcttc ttaagtgctt ccaattgccg gagcgccgcc
     1681 tcattgttac cgtcatactc ttcattgaca tgcacgcggt aagttgccgc ccaggcatta
     1741 gtcggttctc gtttgatcac ctgatcgagc tgattgatgc ttttctgata gtactgctta
     1801 atctgcggta tagcccaggt cggagcgccc tcgagcgccc ccggaatatg aggaacggct
     1861 cctaccggcg gctttgtacc ggtgagagcg gaagcaagaa tggtggcacc gttgagcaag
     1921 ttatcaccca gatggctttt cttgcggtac atctgcggca ttatcggttg cttagctata
     1981 ctctcttgct tgcgagcctg ctcccccaat aaataagcct gcatataata agcccaactg
     2041 agaccatagc gcacacagga attatttgga tcggcatcca gagattgttc atactgcgaa
     2101 acggcgcgat tgaaataatt tggatcgccc gtcgctctgg ccagattgcg cgcacgtcgc
     2161 acttccttca aggccgcctc cacctggctg gagcgcgtca gcgctacggc gtgaatgaag
     2221 gccgcctcag cgctatcggg aaactcggaa gcctga
//
LOCUS       DAOQNZ010000651         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29960_length_2255_cov_5.075909, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000651 DAOQNZ010000000
VERSION     DAOQNZ010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29960_length_2255_cov_5.075909"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1122)
                     /locus_tag="PKW73_11710"
     CDS             complement(43..1122)
                     /locus_tag="PKW73_11710"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNA74001.1"
                     /translation="MFVKKSGLTESDVKKALSTVMDPDLNIDVVSLGFISEIQINGGK
                     VSFKLTLTTPACPVKEKMEQECKDAVLALPGVETVEMETLAQVPNQRKNGDKEPIPGI
                     KHLIAVTSGKGGVGKSTVSVNLAVALAKLGAKVGILDADITGPNVPIMMGVDNYEPTA
                     KDSRIIPAENHGVKCISMAFFVPKDTPLIWRGPMLDKGIRQFLRDVEWGELDYLIVDM
                     PPGTGDAQLTMASATQLSGGVIVTTPQEVALLDGMRGLTMFQKMEVPILGFIENMSYF
                     QPKGSSEQFEIFGRGGGKRLAEETEVPFLGEIPLDPAVRMGGDSGVPIVEADADSPVT
                     RAFMEIAKQVAAQVSIHNHAALATA"
     gene            complement(1204..>2255)
                     /locus_tag="PKW73_11715"
     CDS             complement(1204..>2255)
                     /locus_tag="PKW73_11715"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74002.1"
                     /translation="CFKQGDYPRAETYMKEALEQCRSFGPDYQYDKRTVRVTNNLAEL
                     YRQQGRYQEAEPYYRQTITAAARNFPENRPERAVVHNNLAALLREMGRYQEAEAEYKQ
                     ALSIWQTKVKQPDSPQLAKFLCGMAKLKCDQGQYKEAENLYFQALKLNERVVGKGDVD
                     NAYILANIGGLYRETRQYEKADSYYREALKLDLAVLGQDHPDTACDYNNLGANLREQN
                     KLAEALPYFDKALKIRLSKLGRQHRLTAKTLMGQAELQRRLKHLESAETLVRHALDIQ
                     SKCLPPNHPEIAEANDILGLILEDAGGSKSEAAVQHFQKALFIREKLLSPDHPDIKSS
                     QEHLQNAMKLAQKPQ"
BASE COUNT          438 a          577 c          639 g          601 t
ORIGIN      
        1 caaagtgatc tcttcaaaac cctatttata tattgcggac cttcaggccg tggccagagc
       61 ggcatggttg tgaatggaga cctgggctgc tacttgcttg gctatttcca tgaaggctct
      121 ggtgaccggg ctatcggcat cggcttccac gatgggaacg ccgctgtcgc cgcccatgcg
      181 cacagccgga tcgaggggaa tttcgcccag gaaaggtact tccgtttcct cggccaggcg
      241 cttaccgccg cctcttccaa agatttcaaa ttgctcgctt gagcctttgg gctggaaata
      301 actcatattt tcgatgaagc ccagaattgg cacttccatt ttctggaaca tggtgagacc
      361 gcgcatgccg tccagaagcg ctacttcctg aggggtggtg acgatgacgc cgccggagag
      421 ctgggtggcg gaagccatgg tcagttgcgc atcaccggta cccgggggca tgtccacgat
      481 caagtaatcc agttcgcccc attccacgtc gcgcaggaac tggcgaatac ctttgtcgag
      541 catgggaccg cgccagatga gcggggtgtc cttgggcacg aagaaggcca ttgagatgca
      601 ttttacgccg tgattttcgg cgggaataat gcgggaatct ttggctgtgg gttcgtagtt
      661 atccacaccc atcatgatcg gtacgttggg accggtgata tcggcatcga ggatgcccac
      721 tttggcgccg agcttagcca gggccacagc caggtttacc gagaccgtcg acttgcccac
      781 tccacccttg cctgaggtga cggcaatcag atgtttgatg cccgggattg gttccttatc
      841 gccgttcttg cgctgattgg gaacctgggc cagggtttcc atttccacgg tttcgacgcc
      901 ggggagagcc agcaccgcat ctttgcactc ctgttccatc ttttctttga cgggacaggc
      961 cggtgtggtc aaagttagtt tgaaagaaac tttgccgccg ttaatctgaa tttcggaaat
     1021 gaaaccgagg gagaccacgt cgatattgag gtcggggtcc atcactgtgg aaagcgcctt
     1081 tttgacatcg ctttcagtaa gtccgctctt cttcacaaac ataatgcacc ttaaactctc
     1141 gagacactga catcgtcaga gatcatagcc tcaaaatgag caggtgagcg gcaacctaat
     1201 aaattattga ggcttttgag ccagtttcat ggcgttttgc aaatgttcct ggctgctctt
     1261 gatgtccgga tgatcggggc tgaggagttt ttcgcggatg aaaagagcct tctggaagtg
     1321 ctgtacggcg gcttctgatt tagaaccgcc ggcatcttcc agaatcagtc cgagtatgtc
     1381 attggcttcg gcaatctccg gatgattcgg aggcaggcac ttgctctgaa tgtccagggc
     1441 atgtctgact agtgtttcgg ccgattcgag atgcttcagt cgtctttgca gttcggcctg
     1501 tcccatgagg gtcttggctg tcaggcggtg ttgtcttccc agcttggaaa ggcgaatctt
     1561 gagagcttta tcaaagtaag gaagggcctc ggccaatttg ttctgttctc tcagattggc
     1621 tcccagattg ttatagtcgc aggctgtgtc cggatggtcc tgacctagaa cggccagatc
     1681 gagtttgagg gcttcccggt aatagctgtc ggctttttcg tattgtctgg tttcgcggta
     1741 gagccccccg atattggcaa gaatataggc attgtccaca tcacccttgc ccaccactct
     1801 ttcattcagt ttgagggcct ggaaataaag attctcggct tctttgtact gtccctggtc
     1861 gcatttgagt ttggccatgc cgcagagaaa cttggctagc tgcggtgaat ccggttgttt
     1921 gacctttgtc tgccagatgg acagcgcctg tttgtactcg gcttcggctt cctgatagcg
     1981 ccccatctct ctcaagaggg ccgctagatt attgtgcacc actgctcttt ccgggcgatt
     2041 ctccggaaaa tttctggcgg cggcggtgat ggtctggcgg tagtagggct ccgcctcttg
     2101 gtagcgacct tgctgtcggt agagttcggc tagattgttc gtgaccctca ccgtgcgctt
     2161 atcgtactgg tagtcgggac caaagctgcg gcattgttcc agggcttcct tcatataggt
     2221 ttcggcccgg ggatagtcgc cttgcttgaa gcagg
//
LOCUS       DAOQNZ010000652         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_29966_length_2255_cov_3.732273, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000652 DAOQNZ010000000
VERSION     DAOQNZ010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29966_length_2255_cov_3.732273"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKW73_11720"
     CDS             complement(<1..665)
                     /locus_tag="PKW73_11720"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNA74003.1"
                     /translation="MITTSSENLPWQPIFTIERSRKAEVTVYGIVSVVTGDDNFADFA
                     RPLEQRYRALVSHGKTDFELWTRSLLKPWQLLSHMDTLKRNYPDLKDEHYALFMASHS
                     AEPCHMEALETVMKLTGINEESLRCPQAAPLCQESKDLMKSRGEKPRRRYHNCSGKHS
                     GYLAALKAMGQKPESYLDENNPHHDDLKEILAYFTGRPTSSFVATTDGCQLPNYALST
                     FEIA"
     gene            840..1265
                     /locus_tag="PKW73_11725"
     CDS             840..1265
                     /locus_tag="PKW73_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNA74004.1"
                     /translation="MIKEAVNPGQASPSPLGAYSHALRVGDLLFISGQGCRDPKTGVE
                     CGLTLDEKGQVLTYDIEEQTRGVLENLGVVLRHAGLDYSDLVDMTVFLHDMQDFERYN
                     KVYAGYFAGVAVPPTRTTVQVARLPGRNYIEIKAIASFG"
     gene            1283..>2255
                     /locus_tag="PKW73_11730"
     CDS             1283..>2255
                     /locus_tag="PKW73_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA74005.1"
                     /translation="MTANSAVKSGAKLKEVHHFIGGEFVPGESAETFESFSPGTGKPH
                     ARVALGTARDVDRAVSAALDAQKGPWAKMPMKERCQILRRIGDLILENKEELAIAEST
                     DNGKPLSESLEGDIPRSAMNFHFFSEFAASHVEEAFTVSSSERHVAVREPLGVCGLIT
                     PWNLPLYLATWKIAPALAMGNTIVLKPAEWTPYSAFLFADLARQAGLPPGVFNIVHGF
                     GADGAGEALTRHKDVKSISFTGETSTGKAIMMAAAPTLKKLSFELGGKGANIIFNDAN
                     LEEAIPTAVRAAFRNQGQICLAGSRLFVQRKVYDQVVEKMVERVKAIK"
BASE COUNT          482 a          555 c          660 g          558 t
ORIGIN      
        1 gctatttcaa aagtggaaag ggcataattg ggcagttggc aaccatcggt ggtggcaaca
       61 aaggagcttg tggggcggcc tgtaaagtag gccaggattt ccttcaggtc atcgtgatga
      121 gggttgtttt catcgagata actctccggc ttttgaccca tggcttttaa ggccgccaga
      181 taaccggaat gtttgccgct gcaattgtga tagcggcggc ggggtttttc gccccggctc
      241 ttcatcaggt ctttgctttc ctggcaaagc ggcgcggcct gggggcagcg cagggactct
      301 tcgtttatgc cggtgagctt catgacggtt tcgagggctt ccatatggca gggctcagcc
      361 gaatgggaag ccatgaagag agcatagtgc tcatctttga ggtccggata gttccttttg
      421 agagtatcca tgtgcgaaag taactgccat ggtttgagca gggaacgggt ccatagttca
      481 aagtctgttt tgccgtggct gacaagggcg cggtatcttt gctcgagtgg tcttgcgaaa
      541 tcggcgaaat tgtcgtcgcc tgttactacg gagacgatac cgtagacagt cacttccgct
      601 ttcctgcttc tctcgatggt gaaaataggc tgccagggta ggttttcaga acttgttgtg
      661 atcattgccg taaccatatt ggggtagcgt tccttgttgc ctctgtatgc taattattct
      721 ttcaggaacc gtgaggtgcg gcccctagag cataagattg agtgtcccaa gtttaaatga
      781 ccttgggggg tttcaactag tgctgctcat ttaaggagcg gcagcgcagg agttctggaa
      841 tgattaaaga ggcggttaat ccgggccagg cttcacccag ccctctgggt gcctattctc
      901 atgccctccg ggtgggtgat ttgcttttca tcagcggcca gggttgtcgg gatcccaaga
      961 cgggagtgga gtgtggactt actctggatg aaaaggggca ggtgctgacc tacgacatcg
     1021 aggagcagac ccgaggcgta cttgaaaatc tgggagtggt tttgcgccat gccggccttg
     1081 actacagcga cctggtggat atgaccgttt tccttcacga tatgcaggac ttcgagcgct
     1141 ataacaaggt ttatgccggt tattttgccg gtgtggcggt gccgcctact cgcaccactg
     1201 tgcaggtggc gcggctgccc ggacgtaact atatagaaat taaagcaatt gccagcttcg
     1261 gctaaataag gagagctaaa agatgacggc caatagtgca gttaaatccg gggctaaact
     1321 gaaggaagtg caccacttca tcggcggaga atttgtgccg ggtgaaagtg ccgagacttt
     1381 cgaaagtttt tctccgggaa ccggcaagcc ccatgcccgg gtggccctgg gtacggcccg
     1441 ggatgtggac cgggctgtct ccgctgctct tgatgctcag aaaggtccct gggccaaaat
     1501 gcccatgaaa gaacgctgtc agattttgcg gcgcatcggc gacctcattc ttgagaacaa
     1561 agaagagctg gccatcgccg aatctactga taacggcaag cccctcagtg aaagtctgga
     1621 aggggatatt cccagatcgg ccatgaactt ccacttcttc tcggaattcg ccgccagtca
     1681 tgtggaagaa gccttcaccg taagctctag cgaacggcat gtggccgtga gggaacctct
     1741 aggggtatgc ggactgatca ctccctggaa tctccccctt tatcttgcca cctggaagat
     1801 tgctccggct ctggccatgg gcaataccat agttttgaag ccggctgaat ggactcctta
     1861 cagtgccttt ctctttgccg acctggcccg tcaggccggt ctgccgcccg gtgtcttcaa
     1921 catcgtgcac ggtttcggtg ccgacggtgc cggtgaggcc ttgacccgcc ataaagacgt
     1981 caaatctatt tcctttaccg gtgaaacctc taccggtaag gccattatga tggctgccgc
     2041 tcccactctc aaaaagctat ctttcgaatt gggcggcaaa ggcgccaaca ttattttcaa
     2101 cgatgccaat ctggaggaag cgataccaac ggcggtgcgg gccgcttttc gcaaccaggg
     2161 gcagatttgt ctggccggct ctcgtctttt cgtacagcgt aaggtctatg accaggttgt
     2221 ggagaaaatg gtggagcggg tcaaagccat caagg
//
LOCUS       DAOQNZ010000653         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30069_length_2251_cov_3.341075, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000653 DAOQNZ010000000
VERSION     DAOQNZ010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30069_length_2251_cov_3.341075"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..>2251
                     /locus_tag="PKW73_11735"
     CDS             137..>2251
                     /locus_tag="PKW73_11735"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNA74006.1"
                     /translation="MKAGLKHQVGTNSSRFFKSLSLVSLTILVFCLQLMLALPAYAAS
                     PVGVLKSGRNAAVYQDQHIGTFEDDFKVFKSAIDGANVRFDELSDGDIEAGQAKLAGY
                     KLIVVPLLVDLPQSEVTALSNYQQAGGKLVITDGAGQAGAGAQALMALAGVQALKETT
                     LKEVGKLNWPREPLPTNAEFAIASVVAELTVSPAAKALANWQDASGNNTAAAITRLGN
                     AAYLGWAPGVQGEISSNAQLLSQVLDELSPGITQQAAVQISYAEYQTLSNELDYLAKR
                     TDEAIKTAKQAEFSVPLKTIQGFYDSGLEHVKAFNEAYRERRFLEADERLQKARQDFA
                     MAFAQSMPVRPVEARCIWLDRGTIVATKGPKGMADLFDKLKTAGINSVYFETNNAGFT
                     MYPSKMAVQNPETIGWDPLGTALAEARKRKMEFHAWFWIFNVGNAKHNPIIGKESDYP
                     GPVLSSHDLVWALTSNTGSFLPPRQYEYWLDPSNPECRKYSKDLILEVVNSYKVDGIQ
                     LDYIRYPFNNRGSEMGWNWLGRQRFERETGLSLDRLDDDTRQMWVHWKTAIVNNYVKD
                     MSDTLRALQPGIRISAAVYAFPKRMRLNAIQQEWETWVANGWVDTINPMTYVASPKEL
                     TEQAAFVRESTQDKGLAFPGLSIRQLDTAGLIEQLDSARVTGTLGTTMFAVAHLDEKK
                     VGVLKVGPYRRATILTPQAEPLK"
BASE COUNT          532 a          605 c          601 g          513 t
ORIGIN      
        1 ctggttaaag ttgacctggc cgtggcaaaa gggcgaaaac ttcacgataa gcgcgatgca
       61 atttctcaga aagaaaccaa gcgtcagcta gatagagtaa ttaagaccag tcgcaatatg
      121 aatgtatagg ggaattatga aggcaggact aaagcaccag gtgggaacta attccagtcg
      181 cttctttaag tcactatctc tggtatcact gaccatactg gttttctgtc tgcagttgat
      241 gctggccttg cctgcctatg ccgcttcacc ggtaggtgta ttgaagagtg gtagaaatgc
      301 cgctgtctat caagatcagc atatcggcac ttttgaggat gacttcaagg tcttcaaatc
      361 agccatagac ggtgccaatg tgcgcttcga cgaactgagc gacggcgaca tcgaagccgg
      421 tcaggccaag ttggccggat acaagctgat tgttgttccc ctgcttgtgg atttgccgca
      481 aagtgaagtg acggcgcttt ccaattatca acaggccggc ggcaagcttg tcatcaccga
      541 tggtgccggt caagccggcg ccggtgccca ggccctgatg gccctagccg gtgtgcaagc
      601 tttgaaagag actaccctga aagaagtcgg caagttaaat tggcctagag agcctctgcc
      661 caccaatgcc gaatttgcca ttgcttcggt ggtggccgag ttaaccgttt ctcctgccgc
      721 caaggcgctg gccaactggc aggacgcttc cggcaataat accgccgccg ccattacccg
      781 cctgggcaac gcggcctacc tgggatgggc accgggggtg cagggtgaaa tatccagtaa
      841 tgcacagcta ctctctcaag ttcttgatga actttctcca ggcatcaccc agcaagcggc
      901 ggtgcagata agttatgccg aataccagac cctttccaat gagctggatt atctagccaa
      961 gcgtactgat gaagccatca agacagccaa acaggctgag ttttccgtgc ccctcaagac
     1021 cattcagggt ttctacgact ccggtcttga gcatgtgaaa gccttcaacg aagcttatcg
     1081 ggaaagacgg tttctcgagg ccgatgagcg cttgcagaaa gcccgccagg attttgccat
     1141 ggcctttgcc cagtctatgc cggtgagacc ggtggaagcc cgctgcatct ggttggaccg
     1201 cggcaccatc gtggccacca agggccccaa aggcatggcc gacctcttcg ataagttgaa
     1261 aaccgccggc atcaactcgg tttattttga gaccaacaac gccggcttca caatgtatcc
     1321 ctccaagatg gccgttcaga atcctgagac cattggctgg gatccgctgg gaaccgctct
     1381 tgcggaagcc agaaaacgga aaatggaatt tcatgcctgg ttctggatct tcaatgtggg
     1441 caatgccaag cacaatccca tcattggcaa agagtcagat tatcccggcc ccgtactgtc
     1501 cagtcatgac ctggtctggg cccttacttc caataccggc tccttcctgc ctcccaggca
     1561 gtatgaatac tggctcgacc cctccaaccc cgaatgccgc aaatattcca aagacttgat
     1621 tctggaagtg gtgaacagct acaaagtaga cggcattcag ctcgactaca ttcgttatcc
     1681 tttcaataac cgtggttccg aaatgggttg gaactggctt ggccgccagc gcttcgaaag
     1741 agaaaccggt ttgtctctgg atcgtctcga tgatgacacc aggcagatgt gggtgcactg
     1801 gaagacggcc atcgtcaaca attacgtcaa ggatatgtcc gataccttgc gtgccttgca
     1861 gcccggtatt cgcatatccg ccgctgttta tgcctttccc aagcgcatgc gcttgaatgc
     1921 tattcagcag gaatgggaaa cctgggtggc aaatggttgg gtggacacca tcaatcccat
     1981 gacctatgtg gcttcaccga aggaactgac tgaacaagct gctttcgtgc gtgagtcgac
     2041 ccaggataag gggctggcct tcccggggct ttccattcgg cagctggata cggccggttt
     2101 gattgaacaa ctggactcgg cgagggtgac aggtaccctt ggtaccacta tgtttgccgt
     2161 agcccacctg gatgagaaaa aggtgggtgt cctgaaggtt ggtccttatc gccgtgccac
     2221 cattttgacg ccgcaggccg agccgctcaa a
//
LOCUS       DAOQNZ010000654         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30073_length_2251_cov_3.143898, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000654 DAOQNZ010000000
VERSION     DAOQNZ010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30073_length_2251_cov_3.143898"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1223)
                     /locus_tag="PKW73_11740"
     CDS             complement(48..1223)
                     /locus_tag="PKW73_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HNA74007.1"
                     /translation="MYKILTLNNICVAGLERFPRDKYEIASEISKPDAILLRSYNMHD
                     YAAPSSLKAIGRAGAGVNNIPVAEYTQRGIPVFNAPGANSNAVKELVLAAMLLSARNI
                     GPAWEFARSLNGSDEEIDKIVEAKKKDFVGIELSGRTLGVVGLGAIGVKVANAALALG
                     MKVIGYDPHITVTNAWQLHSDVKGTASMSEVLASSDFVTVHVPLVDATRNIIDAKRLA
                     KARPGITILNFARPGIADEEAVLAAINSGKVRSYICDFPTNLLKDCSRVITLPHLGAS
                     TEEAEENCAVMVAEQVRDFLELGTIRNSVNFPDMHLPEGDAAHRMLVSNANVPHMIER
                     ISAAVANANINIVDMLNRSRGEVACTMLDLQSPVPQSVLEEISAIKGVLNVRELKLR"
     gene            complement(1226..>2251)
                     /gene="serC"
                     /locus_tag="PKW73_11745"
     CDS             complement(1226..>2251)
                     /gene="serC"
                     /locus_tag="PKW73_11745"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR01364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNA74008.1"
                     /translation="EMLDWHGTGLSVMEMSHRSKDFAQIAEEAEADLRELLNVPAQYK
                     VLFLQGGASGQFAAIPMNLLAGKDRADYLVTGFWSQKAYEEAQKYCKANLVASGQSGH
                     FQAVPEPDSWRLSKGAAYFHYTDNETIHGVEFPFVPEVTCPLVVDLSSNILSKPIDVS
                     RYALIYAGAQKNFGPAGLTLVIVHEDYLGRALPNTPSIFDYTAQAANDSMVNTPPTYG
                     WYIAGLTFKWLKKLGGLKEMEKLNIAKAKILYDAIDGTRHFENRVAKECRSLMNVPFF
                     IKTEVLEKQPELEKRFLDEAKKARLLTLAGHRSVGGMRASIYNAMTREGVQALADFIL
                     QFDESIK"
BASE COUNT          493 a          580 c          615 g          563 t
ORIGIN      
        1 accagcatcg aaaccctctg ctatttccac aacgcggagc cttgagctta gcgtagcttc
       61 aactcgcgca cgttgagcac acccttgata gcggaaattt cctccagcac cgactgcggt
      121 accgggcttt gcagatcgag catggtgcag gctacttcac cgcgggaacg gttcaacatg
      181 tccacgatgt tgatattggc attagctaca gccgccgaga tgcgttcgat catgtggggt
      241 acgttggcat ttgaaaccag catgcgatgg gcggcatctc cttccggcag gtgcatgtcg
      301 gggaaattaa cggagttcct tatggtgccc agttccagaa aatcacgcac ttgctcggcc
      361 accatgactg cgcagttttc ttccgcttct tcggtagaag caccaaggtg cggcagtgtg
      421 attacccgag agcaatcctt caagagattg gtgggaaagt cgcaaatata gcttctcacc
      481 ttgccgctgt tgatggcggc caaaacggcc tcttcgtcgg caatgccggg gcgggcaaaa
      541 ttgagtatgg taatacccgg tctggctttg gccaggcgct tggcgtcgat gatgtttctg
      601 gtggcatcta ccaggggcac gtgcactgtg acaaaatcgg aagaggccag tacttcactc
      661 atggaagccg tgcctttgac atcggagtgt aactgccagg cattggtgac cgttatgtga
      721 gggtcataac ctataacttt catgcccagg gcaagggccg cattggccac cttgacgccg
      781 atggcaccaa gtccgaccac gcccagggtg cgtccggaaa gttcgatgcc tacgaaatct
      841 tttttcttgg cttcgacaat tttgtcgatt tcctcgtcgg agccattgag ggagcgggcg
      901 aattcccagg ccggaccgat atttctagca gataaaagca tggctgccaa aaccagttct
      961 ttcacggcgt tagagttggc tcccggcgcg ttgaagaccg gtattcctct ctgggtatac
     1021 tctgccaccg gaatattgtt gaccccggct cccgcccttc ctatggcttt gagggaggac
     1081 ggtgctgcgt agtcgtgcat gttgtaggag cgcaataaga tggcgtccgg tttgcttatc
     1141 tcagaagcaa tctcatattt gtcacgggga aaacgctcaa gacctgctac gcagatgttg
     1201 ttgagggtaa gaattttata catgtttact ttatgctttc gtcgaactgt aagatgaagt
     1261 cagccagggc ctgcacccct tctctggtca tggcgttata gatgctggca cgcatgccgc
     1321 ccacgcttct gtgaccggcc agggtcaaga ggcgcgcctt tttggcctcg tctaaaaagc
     1381 gtttttccag ttccggctgt ttttccagaa cttcggtctt gataaagaag ggaacattca
     1441 tcaaggagcg gcattcctta gcgactctgt tttcaaagtg cctggtaccg tcgatggcat
     1501 catataagat cttggctttg gcgatattga gcttttccat ttctttaaga ccacccaatt
     1561 tcttcaacca tttgaaggtg agaccggcta tgtaccagcc gtaggtgggc ggtgtattaa
     1621 ccatggaatc gttggctgct tgagccgtgt agtcgaaaat agaaggcgta ttgggcaggg
     1681 ctctgccaag ataatcttca tggacaatca ccagggtcaa gccggccggt ccgaaattct
     1741 tctgggcacc ggcgtagatc aaggcatagc gactgacgtc tatgggttta gaaaggatgt
     1801 tggacgataa atccaccacc agcgggcaag tcacctcagg tacaaaagga aactccacac
     1861 catggatggt ttcattgtcg gtgtaatgaa aatacgccgc gcctttgctg aggcgccagc
     1921 tatccggctc cggtaccgcc tggaagtgcc cggactgtcc gcttgccacc agattggctt
     1981 tgcagtattt ttgcgcttcc tcataagcct tctgagacca gaagcctgtg accagatagt
     2041 cggctcggtc tttgccggcc agaagattca tggggatggc tgcaaactga ccggaggctc
     2101 cgccctggag gaagagtact ttgtactgag ccggtacgtt caacaactct ctgagatcag
     2161 cctccgcctc ttcggcgatt tgggcgaaat ctttggagcg atggctcatc tccataacag
     2221 agagtccggt gccgtgccag tcgagcattt c
//
LOCUS       DAOQNZ010000655         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30110_length_2250_cov_3.188155, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000655 DAOQNZ010000000
VERSION     DAOQNZ010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30110_length_2250_cov_3.188155"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKW73_11750"
     CDS             <1..527
                     /locus_tag="PKW73_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74009.1"
                     /translation="AMACLARELRWLDEVDEAIEIYETLLALNPNDNQGVRYELAPCL
                     FEAGKYEALEAHLRKYGSESAAIFLYTRALYLFQKTGNSKEARDALQRAFKRNAYVPI
                     FLSDVVEMPDERPTFIGIGDENEALSYVMEYSYLWEPEQTAWLANTLEKDLYKSFADD
                     PELVSDVLKELRLE"
     gene            571..>2250
                     /locus_tag="PKW73_11755"
     CDS             571..>2250
                     /locus_tag="PKW73_11755"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HNA74010.1"
                     /translation="MHRPKAAAALISLLVLHTVLPVAAADRLDSPRLVLKNNSHFLVV
                     APDGTMTLPGAASRSLPYGLYKDDTRYLCGLRLKIDGLAPTLLTAATDQGYSGKFTYR
                     AGKANQKSGKGLLLAREVVIQDGLSERITIKNEGEKPASCKLTMSFDCDFKDMFEVRG
                     QKRKARGKFKKLVEVVKEKDKQSSLLAQYTGLDGRLMSTTIELSGLTAGFRGGILQQQ
                     IEILPHGQMQFEWQARTNLVGSGTTDEGANSPAGLSPFLTAKEKAEQEYKSWQEQIAT
                     IECDDAALNKVFQQARRDLFLLSLSTEKGPALAAGLPWYAVPFGRDQIITARQILPFA
                     PVVVKGLIKTLAAYQGKRDDPYTEETPGKIMHELRSGEMARTREIPFIPYYGTIDATP
                     LWLVLVGEYFERTEDEKLLQELKPNLQAAIDYLKRSTKDVYLYYGEKADKHKKAALTN
                     QAWKDSGDSVMHKDGSPAKAPIAICEVQGYLYQAWQTAARLSKILKDERQASDLESRA
                     AHLKERFHQDFALESENFFALAKDATPEGCKVVASNAGHLLATGILEKKEEE"
BASE COUNT          634 a          595 c          591 g          430 t
ORIGIN      
        1 aggccatggc atgtctggcg cgagagctta gatggctgga cgaagtggac gaagccattg
       61 aaatttacga aacccttctg gcgctgaatc ccaacgataa tcagggagtg cgttatgagc
      121 tggcgccctg cctctttgaa gcgggcaaat acgaagcact ggaagcccat ctgcgcaaat
      181 atggctccga gtctgcggca atttttctct acacgagagc gctctatctc ttccagaaaa
      241 ccggtaacag caaggaagca agagatgcgc tccagagagc cttcaagcgc aacgcctatg
      301 ttcccatctt cctctccgat gttgtggaaa tgcccgacga aagaccgact ttcatcggca
      361 tcggagacga gaacgaagcc ctttcttacg taatggaata ttcctacctc tgggaaccgg
      421 aacaaacagc ctggttggct aataccctgg aaaaggacct gtacaaatca tttgccgatg
      481 atcccgaact ggtaagcgat gttctcaagg aactgaggct ggaatagccg ctcagtcagc
      541 gaaagatagc ggaagaaagg tagaacttaa atgcatcgac caaaggcggc cgccgctcta
      601 atcagcctgt tagtgctgca cacggtgctg ccggtagccg cggcagacag actggatagc
      661 cccaggctcg tgctcaagaa caactctcac ttcctggtag tggctccgga tggaaccatg
      721 acgctgccgg gggcggccag tcgctccctg ccctatggac tgtacaaaga tgatacccgc
      781 tatttatgcg ggctcagact gaaaatagat ggtctggcac cgactctcct aactgccgcc
      841 accgaccagg gttatagtgg taagttcact taccgggcag gcaaagcaaa tcaaaagtca
      901 ggtaaggggc tgctgctcgc acgagaggtg gtcattcaag acgggctcag cgagagaatc
      961 acaattaaaa acgagggcga aaaaccagcc tcatgcaagc tgaccatgag cttcgactgt
     1021 gactttaaag acatgttcga agtacgtgga cagaagcgca aagccagagg caaatttaag
     1081 aagctggtgg aagtcgtgaa agaaaaagac aagcagtctt cccttctggc tcagtacacc
     1141 ggtcttgatg gcagactgat gagcaccacc atcgagctgt ccggtctgac cgccggtttc
     1201 aggggcggta ttttgcaaca gcaaatagag atcctccccc atgggcagat gcagtttgag
     1261 tggcaggcaa gaaccaacct tgtgggaagt ggtaccaccg atgaaggcgc caactccccc
     1321 gcaggcctct cacctttcct gaccgcaaaa gagaaggcag agcaagaata caaatcatgg
     1381 caagagcaaa tagcaaccat tgaatgcgat gatgcggcgc tcaacaaagt cttccagcaa
     1441 gcccgccgcg acctcttcct cctgagccta tcgacagaga agggaccggc actggcagcc
     1501 ggtcttccct ggtatgccgt gccctttggc agggatcaaa ttatcacagc caggcagatt
     1561 ctgcccttcg ccccagtagt tgtgaaaggt ctgatcaaga ccctggccgc ttaccagggc
     1621 aaaagggatg acccttacac ggaagaaaca ccgggtaaaa tcatgcacga gctgcgcagc
     1681 ggagaaatgg ctcgcacgag agaaataccc ttcattccct attatggcac catcgacgcc
     1741 acgccactat ggctggtgct agttggcgag tatttcgaga gaaccgaaga cgaaaaatta
     1801 ctgcaagagc ttaagcccaa cctacaggca gccatcgact atctaaagcg cagcacgaaa
     1861 gacgtctatc tgtactacgg tgaaaaagca gacaaacaca agaaagcggc cctcaccaac
     1921 caggcctgga aagactcagg tgattcagtc atgcacaagg acggctcacc ggcaaaggca
     1981 ccaattgcca tatgcgaagt tcagggttat ctctaccagg cctggcaaac ggcagccaga
     2041 ctatctaaaa ttttgaaaga cgaaaggcag gcttcagatc tggaaagccg ggctgcacat
     2101 ttgaaagagc gctttcatca agactttgct cttgaatccg aaaacttctt tgccctggcg
     2161 aaagatgcca caccggaagg ctgtaaggtg gtagcctcca acgccggaca cttgctggcc
     2221 accggaattc ttgagaagaa ggaagaagaa
//
LOCUS       DAOQNZ010000656         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30158_length_2248_cov_3.754674, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000656 DAOQNZ010000000
VERSION     DAOQNZ010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30158_length_2248_cov_3.754674"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PKW73_11760"
     CDS             complement(<1..417)
                     /locus_tag="PKW73_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74011.1"
                     /translation="MTVRRQRGATLGLAAACVLVVIVIGVGFFFLSKIIGGGRQVANA
                     TDAGALNVAKQAIRRGAVTLSSAADQEEFGALCDTPNRLDLLSYNRIVAKAMIVCKNA
                     QDEGTPLAAANAGMIHNQVNMLGNMLKANLVAACANP"
     gene            complement(685..2148)
                     /locus_tag="PKW73_11765"
     CDS             complement(685..2148)
                     /locus_tag="PKW73_11765"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNA74012.1"
                     /translation="MLNELVLKLSASKLDDRFGQLVRESFLLGSLLALFDGLSKRLKS
                     LNFAPRLGQSLNTLSFLILLVLFAALGLPQFASDKEALALIALAAGGIYLVSRLLGSG
                     STFKFNMMDLMVALYLGANIISSISSHYLPQSIKGLSKVAVYTLSYFLLKASLLDRKE
                     RLPWYLSAALLSGLAVSLYGLYQYKIGVAPLATWEDPTVENKGTRIYSTLGNPNLLAG
                     YLLPLAPLALAMSTYIGKAKSKLRFLISPLFLITAIITAVAIFLTGSRGGYLSIIGTG
                     GFIVLALSVYFFSKKPKLIAPSALIFLAVAAALYAALHFLMPTFEQRLASIFAGSEHS
                     SNAYRMHVWRASMAMFADNWWLGVGSGNSAFRLAYGLYMKSGFDALGTYCVPLEVGVE
                     TGILGLVSFGLLILFSFARAHLLFYKDSNLSYLALGLAAALFSLMVQGMVDTVFYRPQ
                     VHFLFWLVIAAISALSSQEESRGTEPEPGDNSEKCQN"
BASE COUNT          560 a          566 c          624 g          498 t
ORIGIN      
        1 ggggttggcg caggcggcaa ccaggttggc tttgagcatg ttgcccaaca tgttgacctg
       61 gttgtgaatc ataccggcgt tggcagcagc caacggggtg ccttcgtctt gagcgttctt
      121 acaaacgatc atcgctttgg cgacgatgcg gttgtagcta agaaggtcga gcctgttcgg
      181 tgtgtcgcaa agagcaccga attcttcctg gtcagcagct gaggacagcg ttacagcacc
      241 ccttctgatc gcttgtttgg cgacgttgag ggcgccggcg tcggttgcgt tagcaacctg
      301 tctgccacca ccgatgattt tggacaggaa gaagaagccg acgcctatca ctattacgac
      361 gagcacacag gcggctgcta agccgagtgt tgcccctctc tgtcgtcgta ctgtcatatt
      421 tttattcctc cacgcaccga ttgctcggct ctttttcgta attagtgctc cggacagacc
      481 gaaggctttg aataagggtc tacgagatta gtaaagtgaa actctgttaa ttatcatcct
      541 ctaatcttgc cctggcgtca agagtctgtt taatctctct ggattccttg taaccctagc
      601 tgggcggcga taaccctgga ttacatgcat ttaatattcc cacaaaacct gggaaatata
      661 gattaaatta gcaacggtta atatttaatt ttggcatttc tcagaattgt cgccgggctc
      721 aggctccgtc ccccggcttt cttcctggct ggatagcgcc gaaatggcgg caatgacaag
      781 ccagaagagg aagtgtactt gaggtctata gaaaacggtg tccaccatac cctgaaccat
      841 taatgagaac agggctgcgg ccagtcccag ggccagatag ctgagattac tatctttata
      901 aaagaggaga tgggctcttg cgaaactgaa tagaatcaag agcccaaaag aaactagacc
      961 aagaatgccc gtctctaccc ccacttccag gggcacgcaa taagtgccga gggcatcgaa
     1021 accgctcttc atatatagtc cgtaggccag acgaaaggcg ctgttgccgg aacctactcc
     1081 cagccaccag ttgtcggcaa acatagccat ggaggcacgc catacatgca tgcggtaggc
     1141 attgctggaa tgctcggaac cggcaaatat ggaggccagt ctttgctcga aggtaggcat
     1201 gaggaaatgc agtgccgcat aaagcgccgc cgccaccgcc aggaagataa gggcgctcgg
     1261 ggcaattagt tttggcttct tactgaagaa atagaccgag agggccagta cgatgaatcc
     1321 gccggtgcct attattgata gatagccgcc ccggcttccg gtcaggaaaa tggccacggc
     1381 cgtgatgatg gcggtgatga gaaagagcgg cgaaatcaaa aaacgcaatt tgctctttgc
     1441 cttacctata tatgtagaca ttgccagggc caggggcgcc agtggcagca gatagcctgc
     1501 cagcaggttg gggttgccca gggtcgagta aatgcgagtg cctttgtttt ccaccgtagg
     1561 gtcttcccag gtggccagtg gtgccacgcc gatcttgtac tggtagagtc cgtagaggct
     1621 gaccgcaagc ccgctcagaa gcgctgccga gagataccag ggcaaccttt ctttgcggtc
     1681 cagcaggctg gccttgagca ggaagtagga gagggtgtag accgccacct tgctcaagcc
     1741 tttgatgctc tgcggcaggt aatgggagga aatgctggaa ataatgttgg cgcccaggta
     1801 aagcgccacc atcaagtcca tcatattgaa cttaaaagtc gagccgcttc ccagaaggcg
     1861 tgaaaccagg tagatgccgc cggcagccag ggcaataaga gccagggcct ctttatcgga
     1921 agcaaactga ggcaagccca gggcggcaaa aagtaccagc agaatcagaa acgacagggt
     1981 attaaggctt tgccccaggc gcggcgcgaa attgagagac ttgagccgct tgctcagacc
     2041 gtcgaagagg gcgaggaggg aaccaagcag aaaggactct ctcaccagtt gaccgaaacg
     2101 gtcatccagc ttggatgcgg aaagcttcag aacgagctcg tttaacactt gcgagatcta
     2161 cttatttagt ggcgccgtcg tagatgtcta caactacacc ttgaactctg tatttgaagg
     2221 cttcaagctc gatctggttg cccacctt
//
LOCUS       DAOQNZ010000657         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30195_length_2247_cov_2.924270, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000657 DAOQNZ010000000
VERSION     DAOQNZ010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30195_length_2247_cov_2.924270"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /gene="katG"
                     /locus_tag="PKW73_11770"
     CDS             complement(<1..1977)
                     /gene="katG"
                     /locus_tag="PKW73_11770"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNA74013.1"
                     /translation="MLVVQATLISMASQALPAMADGAPRSNQFWWPERLDLSPLRQHA
                     AESDPFGKDFNYAKEFQSLDLKQVKADIENVLKTSQDWWPSDYGNYGPFFIRMSWHSA
                     GTYRIYDGRGGAGGGQQRFEPLNSWPDNANLDKARRLLWPVKKKYGRKLSWGDLMVLA
                     GNVSLESMGFKTYGFAGGRKDDWEPDLIYWGPGAKFMSDNRSSGGKLEKPLAATQMGL
                     IYVNPEGPGGKPDPLAAAADIREAFGRMAMNDEETVALIAGGHTFGKAHGAASPDKYV
                     GPAPAGEGLEKQGFGWENKFGTGKGGDTITSGLEGAWSSSPVNFTMAYLNNLFNLDWV
                     QTKSPGGATQWTPKDPAQAKPVPDAHDPAKKHPPIMFTTDLALKFDPEYSRIARRFQQ
                     NPEEFKLAFAKAWFKLTHRDMGPRSRYLGAEVPKEALIWQDPVPAADYKMIEASDVAA
                     LKGKILASGLTVPELVRTAWASAATFRGTDKRGGANGARIRLAPEKNWAVNDPEELSK
                     VLKVLEGIQSDFNKRKDGKKVSLADLIVLGGSAAVEAASKKAGYDVKVPFEPGRTGAS
                     QEQTDVSSVAVLEPTADGFRNYYGKSNERSPLDLLVERANFLTLTVPEMTVLVGGMRA
                     LDANEGGSKLGVLSSRPGVLTNDFFVNLLDMSTRW"
BASE COUNT          490 a          642 c          627 g          488 t
ORIGIN      
        1 ccacctggtg gacatatcga gcaggttgac gaagaagtcg tttgtgagaa ctcccggtcg
       61 actcgacaaa acaccaagct tagagccgcc ttcgttggca tcaagggcac gcatgccgcc
      121 cacaagcaca gtcatttcgg gcacagtaag ggtgaggaag ttagcgcgct ccaccagaag
      181 atccaggggc gatctttcat tactcttgcc gtaatagttg cggaagccat cggctgtggg
      241 ctcaagtacg gcgacggaag atacgtcggt ttgctcttgc gaagcaccgg tgcggccggg
      301 ctcaaaaggc actttcacat cgtaaccggc tttcttggag gcggcttcca cggcggcact
      361 gccacccagc acaatcaaat cagccagaga aactttcttg ccgtctttgc gcttgttgaa
      421 gtcagactga atgccctcaa gcactttcag cactttagag agctcttccg ggtcgttaac
      481 cgcccagttt ttctcgggcg caagacggat gcgcgcacca ttggcgccac cacgcttatc
      541 ggtgccgcgg aaggtagcag cggaggccca ggcagtgcga accagctcag gcacggtgag
      601 accggaggcc aggatcttgc ccttgagggc ggcgacgtcg gaagcttcaa tcatcttata
      661 gtcggcggca ggcaccgggt cttgccagat caaggcttct ttaggcactt cggcacccag
      721 atagcgggag cgtggtccca tatctctgtg ggtcaattta aaccaggcct tggcgaaggc
      781 cagtttgaac tcctcgggat tctgctggaa gcgcctggcg atgcggctgt actcagggtc
      841 aaatttcaag gccaggtcgg tagtgaacat tattggcgga tgcttcttag ccggatcatg
      901 agcatcgggc accggttttg cctgggccgg gtctttcggc gtccactggg tggcgccgcc
      961 cgggctcttg gtttgcaccc aatcaagatt gaacaagtta tttagataag ccatggtgaa
     1021 attcaccggc gaagacgacc aggcgccttc caggccactg gtgatggtat caccgccttt
     1081 gcccgtgccg aatttgtttt cccagccgaa gccctgcttt tcgagccctt cgccggccgg
     1141 cgccggacct acatatttat cgggagaagc agcgccatgg gccttgccaa aagtgtgtcc
     1201 accggcaatc agggctacgg tttcttcatc gttcatagcc atgcgaccga aagcttcgcg
     1261 aatgtcggct gccgcagcca gtgggtcggg cttgccgccc gggccttccg gattaacata
     1321 gatgagtccc atttgcgttg ccgccagggg cttttcaagc ttgccgccgg agctgcggtt
     1381 gtcggacata aacttagcgc cgggacccca gtaaataaga tcaggctccc agtcgtcttt
     1441 gcggccgccg gcgaaaccat aggtcttgaa gcccattgat tcaagggaaa cgttaccggc
     1501 cagaaccatc aagtcgcccc aggagagctt gcgcccatac ttcttcttga ccggccacag
     1561 caagcggcgc gccttgtcga ggttggcatt gtcgggccaa ctgttgagcg gctcgaaacg
     1621 ttgctggcca ccgccggcgc caccacgtcc gtcatagata cgataggtac cggcactgtg
     1681 ccaggacata cggatgaaga agggtccgta gttgccgtag tcggacggcc accagtcttg
     1741 agaggtcttg aggacgtttt caatgtccgc ctttacttgc tttaaatcca ggctctggaa
     1801 ctcttttgcg tagttgaaat ctttgccgaa gggatcagac tcggcggcat gctgcctcag
     1861 tggtgagaga tcaagccgct cgggccacca gaactgattc gaacgcggcg ccccatcggc
     1921 catggccggc aaagcctgag acgccatgga tatgagcgta gcttgaacaa caagcacctg
     1981 cacagatctt cttattaccg gcttcatcat taggttgaac cctctctttt gggcagcact
     2041 ggcaccacgc acaaaactat aaccgcaaca ggcacgcacc tggcggttag ataatcgaaa
     2101 cactttggac tgagaccata tttagactaa ctctaaactt aggcactgtc aaccccactc
     2161 caagcgccaa aatatggggg agactagcac ccttccgggt ctccagaccc gaattcaact
     2221 tcgatataag gttaaatcct gttaccg
//
LOCUS       DAOQNZ010000658         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30241_length_2245_cov_4.931050, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000658 DAOQNZ010000000
VERSION     DAOQNZ010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30241_length_2245_cov_4.931050"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..547
                     /locus_tag="PKW73_11775"
     CDS             113..547
                     /locus_tag="PKW73_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74014.1"
                     /translation="MDVNSAVGENSNWKEPVSILCDYPDPAYPLLFNLERPPGSYLVN
                     GRPMRLLQALSRLGPLTGDHLEFYEHIKDSLRFSFEQRRPQLVFVHGTYAMEFLTAAG
                     LSLEEDYDCIDDVLFLTDNVAPREFLGSYVDFVQFKRKSNGK"
     gene            537..>2245
                     /locus_tag="PKW73_11780"
     CDS             537..>2245
                     /locus_tag="PKW73_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74015.1"
                     /translation="MANEGREFARSYRDDCTKWVPVLVSLSLLVIVLQRLVYWFLHPL
                     FVDSDQSVYLAMAKLILQGKVPYRDFFDFNPPLVMYLNIIPVMVAHLFRIPDPLALSM
                     TMEALHLVSTSFMALIIYRYRQYVPALVFVPFLFVYAYFTTTLENDMGQREHFFTLIF
                     LPFLVLRGLRYAGANPGKIMTAGSGLLCGLVLCLKPQFLFLAVLAEIGFLIGNGALRN
                     LKALEMRFLPVAPLCYLVGLLALPVDCLRIYLEQALPVYTYGISWSSKCFSHMLAGFE
                     YFLQPFCQVTLALFLAFCLLLWESRSVTLVKMRVRLWLMPLIFLCLGSMFNYIQGGQA
                     WTYRLLPMALFAQLLLGFELGVLLSVLLSGSLRRYPALSLPLSILTLLAVSCYAERRI
                     DAYYQEVAGHYMFDLADIGYSGKNPRSDFDATLFSILENSQIGDRIVHIGSGIRPGYP
                     CVLQSRREPGSRYLYFMIVMIEVAREKKPEWKDRFDAIEEKVVQNLGQDILKNKPTLV
                     FLQDLGIRDAMERHNFVTRYLSNYTFIGHVDVTSIYKLTGNKFSPSSISPERRAQIIL
                     PIL"
BASE COUNT          442 a          543 c          575 g          685 t
ORIGIN      
        1 gaagtgatat gttgactgcc gcaaccgata ttgacgcgga tttaatgaaa gaagtggcgg
       61 agtcgttgcg gaagggctga atccgcaccc ggtcttgaac cgctccgaca ggatggatgt
      121 caatagtgct gtcggtgaaa attccaattg gaaagagcct gtatctattt tgtgtgatta
      181 tccggatcct gcttatccct tgttgttcaa tctggagcgt ccccccgggt cctatctggt
      241 caatggcaga ccgatgcgtt tactgcaagc actctcgcgg ctgggaccat tgaccggtga
      301 ccatctagag ttttacgagc acattaagga tagcttgcgt ttttcctttg agcagcggcg
      361 accgcagctg gtatttgtgc atggtaccta tgccatggag tttctgactg cagccggctt
      421 gagtctggag gaggattatg actgtattga cgatgtactc tttttgacgg acaacgtggc
      481 gcccagagaa tttcttggtt cctatgttga tttcgtgcag ttcaagagga agtcgaatgg
      541 caaatgaagg tcgcgagttt gctcgctcct atcgggatga ctgcactaaa tgggtgccgg
      601 tcttggtctc actttccctc ttggtaatag ttttgcagag actggtctac tggtttttgc
      661 atccgctttt tgtggattcc gatcagtctg tttacctggc aatggccaaa ctcattctcc
      721 agggtaaagt cccttatcgc gacttcttcg attttaatcc gccgctggta atgtacttga
      781 acataatacc agtcatggtg gcgcatttgt ttcgcattcc tgatcctctt gctctgtcta
      841 tgaccatgga agctttgcac ctggtcagta cttccttcat ggcgctcatt atctatcgct
      901 accgacaata tgtgccggct ctcgtttttg ttccttttct ttttgtctat gcctatttca
      961 caaccacttt ggaaaacgac atgggtcaaa gagagcattt ctttacgctc atttttttgc
     1021 ccttcttagt gctgcgaggg ttgcgttatg ccggtgccaa tcccggaaag attatgaccg
     1081 ccggtagcgg tttgctttgc ggccttgtgc tttgcctgaa gccgcaattt cttttcctgg
     1141 cggttctggc tgaaataggt ttcctgatcg ggaacggggc tttgcgcaac ctcaaggccc
     1201 tggaaatgcg ctttctcccg gtggcgccac tgtgttatct ggtcgggctc ttagctctac
     1261 ctgtggactg tttgcgtatt taccttgagc aggcactgcc ggtctacacc tatggcatta
     1321 gttggagcag caagtgtttc agccacatgc ttgctggctt tgagtatttt ctgcagccct
     1381 tttgccaggt taccctggct ctgtttttgg ccttctgcct gcttctgtgg gaaagccgca
     1441 gcgttacgct ggtgaagatg cgtgtgcgct tgtggcttat gcccctcatt tttctctgtc
     1501 ttggttccat gttcaattat attcagggcg gacaggcctg gacttaccgg cttctgccta
     1561 tggctttgtt tgctcaactc ttgcttggct tcgaactggg agttttgctt tctgtcttgc
     1621 tttcaggctc tctgcggcgc tatccggctc tcagcctgcc tttatccatc ctgaccttgc
     1681 tggcggtctc atgctatgcc gaaagacgaa ttgatgccta ctaccaggaa gttgccgggc
     1741 attacatgtt tgacctggct gatatcggct acagtggtaa gaatccacgc agtgatttcg
     1801 atgcaactct cttcagcatt ctggaaaaca gtcagatcgg ggatcgcatt gttcatatcg
     1861 gttccggaat aagacccggc tatccgtgtg tcttgcagtc taggcgggag ccgggttcgc
     1921 gctatctcta ttttatgatc gtcatgatcg aagtggctcg agaaaagaaa ccggaatgga
     1981 aggataggtt tgacgccatt gaagagaagg tggtgcagaa cctgggccag gatattctca
     2041 agaacaagcc tactctggtg ttcttgcaag atctgggcat tagagatgcc atggaaaggc
     2101 ataattttgt gacccgctat ctgtcaaact atacctttat cggccacgtg gacgtgacct
     2161 ctatctacaa gctgaccggt aacaaattca gcccctcctc cataagcccg gaaaggcgtg
     2221 ctcagattat tctgccgatt ctaag
//
LOCUS       DAOQNZ010000659         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30256_length_2245_cov_2.720091, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000659 DAOQNZ010000000
VERSION     DAOQNZ010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30256_length_2245_cov_2.720091"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKW73_11785"
     CDS             <1..414
                     /locus_tag="PKW73_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74016.1"
                     /translation="HASMTKTGTDTGTGTGTETGAASGSETATGTGTGTGAGTGSETG
                     AATGAEGDKGSSDKDKADPAKDKGAKAPEKAPEKAAEKSAPESGKTVKPGAEKEGAAA
                     PEKAKEPAKDAAAKEPAKDTAKAPTAPAKPAKNPG"
     gene            complement(536..850)
                     /locus_tag="PKW73_11790"
     CDS             complement(536..850)
                     /locus_tag="PKW73_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNA74017.1"
                     /translation="MEIFEKHVFVCTSGKTCSLEGGEEICSLLRKEVVEGGLKGKVRI
                     NKAGCFDQCGHGPLVVVYPEQVWYARVKPEDVKEIVQAHLIEGKPVERLFYDGSGRIK
                     QD"
     gene            1205..1729
                     /gene="rplJ"
                     /locus_tag="PKW73_11795"
     CDS             1205..1729
                     /gene="rplJ"
                     /locus_tag="PKW73_11795"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNA74018.1"
                     /translation="MPTKEHKAGVISDLDQFFVNGKVAIVADLSGYTVAELTHFRRSM
                     DKAQAKVKVTKNTLVKIASKNTEFESIEKLAKGPTTVVVGYEDPAAPAKAVVEAIKSL
                     KKGAVKGGVLEGRCLTSEEVKGLADLPSKEQLLASIMGGLDSGARGIAGIFEGLIRDI
                     ALLTEEVAKKNNNA"
     gene            1783..2166
                     /gene="rplL"
                     /locus_tag="PKW73_11800"
     CDS             1783..2166
                     /gene="rplL"
                     /locus_tag="PKW73_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNA74019.1"
                     /translation="MATKISVDELLDVIGSLTLLEAADLKKKMEDKFGVTAAAPMAMA
                     MVAAPGAAAAVEEKTEFDVVLTAVGDKKIEVLKVVREVTGLGLKEAKDLVDSAPKPLK
                     EKVKKEEAEEMKKKLEATGAKVEIK"
BASE COUNT          625 a          587 c          575 g          458 t
ORIGIN      
        1 cacgcttcca tgaccaaaac cggcactgac acaggaacag gcacaggcac tgaaactgga
       61 gcagccagcg gcagcgaaac cgctaccggt acaggtacag gtactggagc aggtactgga
      121 tctgagaccg gtgctgctac cggggctgaa ggagacaagg gcagtagcga caaagacaaa
      181 gctgacccgg ccaaggataa gggtgccaaa gctcccgaaa aggctccgga gaaagccgca
      241 gagaagagcg cacctgagtc cggcaagaca gtaaagccgg gcgcggagaa agaaggcgcg
      301 gccgctcctg agaaggccaa agagccggcc aaggatgctg ccgcgaagga acctgccaaa
      361 gacaccgcca aggctcctac agctccagct aaaccagcca aaaacccggg ataaaatttc
      421 cccggccacg gtttaaccgg gggcttcccg ggtaatttaa aaattagctg gtggttggct
      481 agctgcctct atttgacaaa ctgtcttgag ggcgcctccc accggcactt tattttcagt
      541 cctgtttgat gcgtccggag ccgtcgtaga aaagtcgctc taccggctta ccttctataa
      601 ggtgggcctg aactatctct ttgacatcct ccggtttcac ccgggcatac cagacttgct
      661 cagggtaaac caccaccaga ggaccatggc cgcactggtc gaaacatccg gccttgttga
      721 tgcgcacctt gcccttcaga ccgccttcga ccacttcttt gcgcaaaagc gagcagatct
      781 cctctccgcc ttccagggag caggtcttgc cgctcgtgca cacaaagaca tgcttctcaa
      841 agatttccat gtgataaaac tcatctaaat gaccgttaca cgtcctttat atcagcttgc
      901 cagccattga cggaagtgtc aggggttgta caatgtgccc ttgtatataa agccatgcct
      961 gagcttcaga cattgcctat atatgactaa gaccgcagac agttcggcac tcatttgagt
     1021 ttaatggctg aaaagccgcc agctagaggt cacaaaatcg actcagtcgt tagcgaataa
     1081 ctcgctttgt gatcccggcg gattggttcc tttactggaa acagcaccgg gaattttttt
     1141 ttcggtggaa ccatccaaga gcgttcttac gtaagcactt gagataacca tctaaggagg
     1201 cccaatgcca acaaaagaac acaaggccgg agtcatctcc gatcttgacc aattctttgt
     1261 taacggcaaa gtggctatcg tagctgatct cagcggctat acggtggcgg aactaactca
     1321 ttttcgtcgt tccatggaca aagcgcaggc taaagtcaaa gtaacgaaga ataccctggt
     1381 aaaaattgcc agcaaaaaca ctgagtttga atccatcgaa aagcttgcaa aaggaccgac
     1441 aaccgtagtg gttggatatg aagatcctgc cgcccctgcc aaagccgttg ttgaggccat
     1501 caaaagcctc aagaagggtg ctgtcaaagg cggcgtgctg gaaggcagat gcctgaccag
     1561 cgaggaagtt aaaggcctcg ccgatcttcc ctccaaagag caactgctcg cctccattat
     1621 gggtggtctc gattccggcg ccagaggtat tgccggtata ttcgagggtc tgatcagaga
     1681 cattgccctc ttgaccgaag aagtcgcaaa gaaaaacaac aacgcttagt gagatgactg
     1741 cagacacctg tgtcgcagtc gcaaaaggag aataatctaa aaatggctac taaaatttcc
     1801 gtcgacgagc ttctcgacgt aatcggttcc ctcacccttc tcgaggctgc tgacctcaag
     1861 aaaaaaatgg aagacaaatt cggcgtcacc gctgctgccc ccatggccat ggctatggta
     1921 gctgctcccg gcgctgccgc tgctgttgaa gagaagaccg agttcgacgt tgttctgacc
     1981 gctgtcggcg acaagaaaat cgaagttctc aaagttgtcc gtgaagtaac cggcctcggc
     2041 ttgaaagaag ccaaagacct ggttgattcc gctcccaaac cgctgaaaga aaaagtgaag
     2101 aaagaagaag cggaagaaat gaagaagaaa ctggaagcca ccggcgccaa agtagaaatc
     2161 aagtaaggtg tatgatctat ccttgaggag cctatgctgt taaaggaaag atcaaagatc
     2221 atgcgttgtc cgacctgtgg cacat
//
LOCUS       DAOQNZ010000660         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30353_length_2241_cov_3.333943, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000660 DAOQNZ010000000
VERSION     DAOQNZ010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30353_length_2241_cov_3.333943"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PKW73_11805"
     CDS             <1..852
                     /locus_tag="PKW73_11805"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNA74020.1"
                     /translation="SVLSSRLALRLADYVVTEAGFGADLGFEKFCDIVASTRPELAPA
                     AVVLVVTVKALKLHGGLSEKELKNCTDENLPEHLAAIEKGFVNAERHAHVVQGFGLPV
                     VLAVNRFATDKDAEVEKVLELARSRNFTARAANVWGLGGEGGIELAEAVIAAAEKERD
                     FKPVYKQEDTVLEKLDKIARSVYGAYRVVLTDEALKRLKWLEKYGFDRLPVCVAKTQY
                     SFSDDPEKLGAPSGFTLTVRELRLSAGAGFVVALTGEIMTMPGLPKVPSGANMKVNGN
                     GQVERFY"
     gene            852..1772
                     /locus_tag="PKW73_11810"
     CDS             852..1772
                     /locus_tag="PKW73_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74021.1"
                     /translation="MAVVFLLFVFVLSLPAQALARESPKDFVLPSTVDFLRSPNVKTY
                     SDLSPSALSVADSLGIGDDLKELDRLSREARGDRLSMLAIKQNLTESILSQGFEVRAV
                     ISKIDIEIADSDDLQALLEERRDQAVRLNSIANFISGGVTGILGSAFNLGDINKKVDD
                     SIDLSDGVVQSSLALLALKQQQGEKRLVKGMPNMLSRLFEAKQAVEYPERIWNFINSV
                     PLTGGQTSTRKEVLMAHWRRLGLIDRVRRDQPSEQLRLKSIVGTAPKCRVSIDLLDAR
                     SAMLHDLRAVISEMDNYLLELIQYLRSVKV"
     gene            1819..2124
                     /locus_tag="PKW73_11815"
     CDS             1819..2124
                     /locus_tag="PKW73_11815"
                     /inference="COORDINATES: protein motif:HMM:NF018574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HNA74022.1"
                     /translation="MTSKKEGSASGKGGETEAQKSVEHSFFCESMEDAQALAEELTVM
                     GHKVLSVEKSDDEEHDGFLVETLESTTLSRAAINAGNLQVLGEEFNAVYDGWCVAID"
BASE COUNT          506 a          514 c          648 g          573 t
ORIGIN      
        1 agtgttctgt cttcccggct ggcactcagg ctggccgact atgtggtgac agaggccggc
       61 tttggcgctg atctgggctt tgagaagttt tgcgatattg tcgcttccac tcgtcctgag
      121 cttgcgccgg ctgccgtcgt gcttgtggtg acggtgaaag ccctgaaact gcacggcggc
      181 ttgtctgaaa aagagctgaa gaattgcacc gatgaaaatc ttccggagca tctagctgcc
      241 attgagaaag ggtttgtcaa cgcagaaagg catgctcacg tggtgcaggg ctttggtctg
      301 ccggtggtgc tcgccgtcaa tagatttgcc accgacaaag acgcggaagt agaaaaggta
      361 ctggagcttg cccgcagccg caattttacg gcgcgggccg ccaatgtctg ggggcttggc
      421 ggtgaaggcg gcattgagct ggctgaggcg gttattgctg ctgctgagaa agagcgcgat
      481 ttcaagccgg tgtataagca agaagatacc gttcttgaga aattggataa gattgcccgc
      541 tccgtctatg gtgcttaccg ggtcgtactg accgacgaag ctttgaagcg gcttaagtgg
      601 ttggaaaaat atggtttcga tcgcctccct gtttgtgtgg cgaaaaccca gtattctttt
      661 tccgacgatc ctgaaaagtt aggtgcaccc tccgggttca cattgacggt gcgagaactg
      721 agactctcag ccggagccgg ctttgtagta gccctcaccg gtgaaataat gaccatgccc
      781 ggcttaccga aggtgccttc cggcgccaat atgaaagtaa atggtaatgg tcaagtcgag
      841 cggttttatt aatggctgtt gtttttctgc tgtttgtctt tgttctgtct ttgcctgccc
      901 aggctctggc cagggagagt ccaaaggact ttgtcctgcc ttccaccgta gactttctgc
      961 gctctccaaa tgtaaaaacc tatagtgacc tgtcacccag cgctctgtcg gtggctgatt
     1021 cgcttggtat aggcgatgac ttgaaggaac tggatcgact ttctcgggag gctcgcgggg
     1081 atagactcag tatgctggct atcaagcaga atctgactga gtcgattttg tcgcagggtt
     1141 ttgaagtgcg agcggtaatt agcaagatcg atattgaaat tgccgattcc gatgatttac
     1201 aggctctcct ggaagaacgc cgggaccagg ctgtacgctt gaactccatt gccaacttca
     1261 ttagcggcgg tgtcaccggt attcttggta gtgccttcaa cctcggcgat atcaataaaa
     1321 aagtcgacga cagtatcgac cttagcgacg gtgtggttca gtcgtctctg gccttgcttg
     1381 ctctcaagca gcagcaggga gaaaagcgcc tggttaaggg catgcccaat atgctttccc
     1441 gtttgtttga agccaagcag gcagtagaat atcccgagcg aatctggaat tttatcaact
     1501 cggtgccgct gaccggtggt cagaccagta cccgcaaaga ggtcttgatg gctcactggc
     1561 gcaggctggg tcttatagat agggtcaggc gcgaccagcc ttccgagcaa ctgcggctca
     1621 agtccatcgt tggtacggct cccaagtgcc gtgtctccat cgaccttctt gatgcccgtt
     1681 cagccatgct tcatgatcta agagcggtga tttccgagat ggataactac cttctggagc
     1741 tcatacagta tttgcgtagc gttaaggttt agatgccgga aagaagtcgt ccacgttcca
     1801 aactggagga agtagaaaat gacatcgaag aaagaaggaa gcgcctctgg taaaggggga
     1861 gagacggaag cccagaagtc ggtggaacat tccttctttt gcgagagtat ggaagatgct
     1921 caggcactgg ctgaggaatt gaccgtcatg gggcacaaag tgctgtcagt ggaaaagtcg
     1981 gacgacgaag aacatgacgg ttttctggtg gagactttgg agtcgaccac actcagtcgg
     2041 gctgccatta atgccggtaa tttgcaggtg cttggcgagg agttcaatgc cgtctatgac
     2101 ggttggtgcg tggctatcga ttgattgttc gtttgatatg tttgcgccaa ttagacaaat
     2161 gtaatgcttg cccgcagggc aatttataag agcaatttag tccttcaggg taaaattgcc
     2221 cgttggcatc atgattcatt a
//
LOCUS       DAOQNZ010000661         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30408_length_2239_cov_3.749084, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000661 DAOQNZ010000000
VERSION     DAOQNZ010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30408_length_2239_cov_3.749084"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..>2239
                     /locus_tag="PKW73_11820"
     CDS             358..>2239
                     /locus_tag="PKW73_11820"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA74023.1"
                     /translation="MLRPGKGPDQGKIRLPRQSVAPGVRELQALYEQAKENRGREVEL
                     IWQVPGTYKTYSILVRFDRVTPHPLWQVWEDNGRENKMVWRFETSDINMIFDVIAMLD
                     VSHQKTTGTVQTLNQSQTGLPLTGAPGTPPSGITPRNTGSFPALGQAAGQSRGLVGSS
                     GAVDSGIFSTNTPPQAAGYPPQPPANPTGTGTGWRTVDEGGWKGGDSGWKAADQGTSS
                     GTWKAAEPTQANPFSAGGGANPNMASSGTWRPAGQPGPDPGQFPAPASGPGFGGGVNP
                     LQAAMANLAAGQPPPNTPPLPSSQSLLKANAPVPPQPSPQAALAGGAGQAMPEGSLPA
                     DFFSDAPATIAGAAPAKTTSIQGSIEQNPLNSVLTNIGMQQLTGKLEVMGDTAVGHVY
                     FENGIPKDAQTSTNRGDDAIKELVTWRRGTFLFKNGLRTEMSSVEKSLQTTLLEGVAL
                     LDQLKHLEKAGLVYESILVQKQKNLGEPELKLMLAKGHPLDFQMQKNAYDSLKHKKTF
                     TDLLRDNPMDQTQWAPLLFNLLYCGLIEIKPPAATRGGALDFLGEGKQIIQNLAYNFV
                     RPDTGIVSFEALLYFLEYEFYRYEAYNWPMSLVAFEMSLRRPGSNIGETEPLPPQALA
                     TAA"
BASE COUNT          543 a          710 c          580 g          406 t
ORIGIN      
        1 ttgtcatgat acagtcccga gcagggcatt ttccttaggt aaaaatcaca tctagcgatc
       61 agctaccaat atctatatcc agagagagca gctctgttta tcagacaccc aaccggaaga
      121 gcgaccgccc ctggccccgg ggcaatccaa atcgggaaaa gcttggcaag attgtgttgt
      181 ataaatgcat gaaaatgcac cttttgtggc ttaaactaag taagcttccg gccgacgccc
      241 aagctcctgg caaccccgag gggagccagt gcggccgctc ccgggagcag gagcccattt
      301 actaattaaa ttaactccga atcactcaaa cacaagctag taaagcgctg aaggcaaatg
      361 ctacgaccag gtaaaggtcc agatcaaggg aaaatcagac tgccgagaca atcggtagcc
      421 ccgggcgtgc gtgaactgca agccctttat gaacaagcca aagaaaaccg cggtcgggaa
      481 gtcgagttaa tctggcaggt tccgggcact tacaagactt acagcatcct ggtgcggttc
      541 gaccgggtca ccccccatcc actctggcag gtctgggaag acaacggtcg cgaaaacaaa
      601 atggtgtggc gctttgagac ttcagacatc aacatgatct tcgacgtcat tgccatgctc
      661 gacgtttccc atcagaagac caccggtacg gttcagaccc tcaatcagag ccagacaggg
      721 ctgccactca ctggtgctcc cggcacaccg ccctcgggca tcacgccccg caacaccggt
      781 tcttttcctg ccctcggcca ggcggccggt caatccaggg gtctggtggg ctcatccggc
      841 gccgtagatt cgggcatttt ttccaccaac acaccacccc aggctgccgg ctatccgccc
      901 caaccacccg ctaaccccac aggcaccggc accggctggc gaaccgtaga cgaaggcggc
      961 tggaaaggtg gagacagcgg ctggaaagcc gccgaccagg gcaccagcag cggcacctgg
     1021 aaagcagcag agccaacaca agccaatccc ttcagtgcag ggggcggagc aaatcccaac
     1081 atggccagca gcggcacctg gagaccagct ggacagccag gtcccgaccc gggacagttc
     1141 ccagctcccg cgtccggtcc tggcttcggg ggcggcgtca atcccttgca ggcggccatg
     1201 gccaaccttg ccgccggtca gccaccgccc aataccccgc ccctgcccag cagccagtct
     1261 cttctcaaag ccaatgcgcc ggtaccgccc cagccgtcac cccaggccgc tctagccggt
     1321 ggcgccggcc aggccatgcc ggaaggttcg ctaccggccg atttcttttc cgatgccccg
     1381 gcgaccattg ccggcgccgc cccggccaaa accacatcca ttcaaggctc tatcgagcag
     1441 aatcccctca actcagtgct gaccaatatc ggtatgcagc aactgaccgg caaactggaa
     1501 gtaatgggcg acacggccgt cgggcatgtc tatttcgaaa acggcattcc taaagacgct
     1561 caaacctcca ctaatcgcgg cgatgacgcc atcaaagaac tagtcacctg gcggcgcggc
     1621 acctttcttt tcaaaaatgg actgcgcacc gagatgagtt cggtggaaaa gagcttgcaa
     1681 accaccttgc tggaaggtgt ggccttgctc gatcagctca aacatcttga aaaagcagga
     1741 ctggtttatg aatcgatcct ggtccagaag cagaaaaatc tgggcgagcc ggaattgaag
     1801 ttgatgctgg ccaaagggca tcccctcgac ttccagatgc aaaagaacgc ttacgactcg
     1861 ctcaagcata aaaagacttt tacagacttg ctgcgcgaca atccgatgga tcagacccag
     1921 tgggcaccgc tgctcttcaa cctgctctac tgcggcctaa tagagatcaa gccccccgct
     1981 gccaccagag gcggtgcgtt agacttcctg ggtgagggca agcagattat ccagaacttg
     2041 gcttacaatt ttgtgcgccc ggataccggc atagtcagct tcgaagccct actctacttc
     2101 ctggaatacg agttttaccg ctacgaagct tacaactggc caatgtctct ggtggccttc
     2161 gaaatgagct tgcgccgccc cggctccaat ataggcgaaa ccgagcctct gcctccccag
     2221 gccctggcca cggctgcca
//
LOCUS       DAOQNZ010000662         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30418_length_2239_cov_3.206502, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000662 DAOQNZ010000000
VERSION     DAOQNZ010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30418_length_2239_cov_3.206502"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..919)
                     /locus_tag="PKW73_11825"
     CDS             complement(92..919)
                     /locus_tag="PKW73_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74024.1"
                     /translation="MGISFHITSVREGTIFNVMQFLSLERVTGVLSVRFGRQIPEVTI
                     YFVAGSITTAEFGSIVGDSVLDMLLCQEFAVKEVAFAPGRINQVNEQAIIVRSTNLAS
                     VMLNVSKDVDKCESRPLIYGLLPINSTTGRTAPSVLHILNDFSPWNDSLKALPRSEKD
                     KKAPLPQCVLIHRAMSKGLITYQRPLVSIKALKPLLEILGNLSDKESNNLRGYLRSLL
                     PHPKATHLSIERFYAFASAIESIAQRRSPEVGDRARRAIHMLIQSATKAQEVGAANA"
     gene            complement(982..>2239)
                     /locus_tag="PKW73_11830"
     CDS             complement(982..>2239)
                     /locus_tag="PKW73_11830"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNA74025.1"
                     /translation="RLRLLGVTGTNGKTTTTHLIEHILNFHGHKCGLVGTLGARLPIY
                     EEEDGRPVFKGKSEYLEVHHTTPQACDLQALLHTMADRGLTHVAMEVSSHALALKRVL
                     GTNFASACLTNITQDHLDFHKTMEHYYRSKRILFEMLNTNKTGKAVAVVNLDDALAQE
                     FINATGKEVELLTYGKNPEASIHPRQFHFDFRGTTLCLATPHGDIEFSTRLTGEFNLY
                     NVMAAMAVCLGEGLTKEQVASALKDFGGVSGRFEVVPPPSGSERQPLCIVDYAHTPDG
                     LENVLKAARALVENQGSGRLIAVFGCGGDRDSSKRPQMGEIAENLADLVVVTSDNPRS
                     EEPQQIIADILAGIKRLKDVTVEADRAKAIAEAVKMARAEDVVLIAGKGHETYQILKD
                     RTIDFDDRIEVANALKQRTDIIKIEK"
BASE COUNT          441 a          567 c          669 g          562 t
ORIGIN      
        1 cagataggta atcaggccca tcttggcttg atgaagccgg tgctcgaagt cgtgcgggtc
       61 ttcttttagt ttctgtctgg ccagtcttcg atcatgcatt ggctgctcct acttcctgcg
      121 ccttggtggc gctctggatg agcatgtgaa tggctcttct ggcccggtcg cctacttccg
      181 gggagcgcct ctgggcgata gactcgatgg ctgaagcaaa ggcgtagaag cgctcgatgg
      241 acaggtgggt agccttagga tggggcagta agctgcgcag atagccccgc agattgttcg
      301 attctttgtc ggataagttg cccagaatct cgagcagggg cttgagagct tttatggaaa
      361 cgagagggcg ctggtaggtt atcagccctt tgctcatggc ccggtggatg aggacgcact
      421 gaggcagcgg cgctttcttg tccttctcgg agcgcggcag ggccttgagg ctatcattcc
      481 atggtgaaaa gtcgttcaag atgtgcagca ccgaaggtgc ggtgcggccg gtcgtgctgt
      541 tgatgggcaa aagaccgtaa atgagcggtc tggattcgca cttatcgacg tctttggaga
      601 cgttgagcat gaccgaggcc aggttggtgg agcgcacgat gatggcttgc tcgttcactt
      661 gattgatgcg cccgggggcg aaagccacct ctttgacggc aaattcctgg caaagcagca
      721 tgtccagcac cgaatctccc acgatggagc cgaattcggc cgtggttatg gagcctgcta
      781 cgaagtaaat ggttacttcc ggtatctgtc tgccgaaacg caccgacaaa acaccggtca
      841 ctctctcaag gctgagaaat tgcatcacat tgaagattgt gccttctcta actgatgtga
      901 tgtgaaaact gattcccaca aagaccgccc ggcagtttgc ttgaactggt attgcctggg
      961 gagattttcc cggtcggcaa attacttctc gattttaatt atatctgtgc gttgcttaag
     1021 tgcgttagct acttcgatgc ggtcatcgaa atctatggtg cgatctttga ggatctggta
     1081 ggtctcatgc cctttcccgg caatcaacac cacgtcctcg gctctggcca tcttcaccgc
     1141 ttccgctatg gctttagccc gatccgcctc tacagtaacg tctttcagcc tcttgatgcc
     1201 ggccaggatg tcggcaatta tctgctgggg ctcctcggag cgggggttgt cggaggttac
     1261 caccacgagg tctgccaggt tctctgcgat ttcacccatc tgggggcgtt tggagctgtc
     1321 tctgtcgccg ccgcagccga agacggcaat tagtcggccg gaaccttgat tttccaccag
     1381 ggcccgggcg gctttcagga cattttccag cccgtccgga gtatgggcgt aatcaacgat
     1441 gcaaaggggt tgcctttcgc tgcccgatgg agggggcact acttcgaaac gacccgacac
     1501 gccgccgaaa tctttcaggg ctgaggccac ctgctctttc gtcaggcctt cgccgaggca
     1561 gacggccatg gccgccataa cgttgtagag gttgaattcg ccggtcagtc ttgttgaaaa
     1621 ctcaatgtca ccatgggggg tggcaaggca caaggtggtg ccgcggaaat caaaatggaa
     1681 ctgacgagga tgaatactgg cctcagggtt cttgccgtag gttagcagtt ctacttcttt
     1741 gcccgtggca ttgataaatt cctgggccag ggcgtcatcc aggttgacca ccgccaccgc
     1801 tttaccggtt ttattggtgt tcagcatttc aaagagaatg cgtttggagc ggtaatagtg
     1861 ctccatggtt ttgtggaagt ccaggtgatc ctgggtgata ttggtcaggc aggcggaggc
     1921 gaaattggtg cccagaaccc tcttgagcgc aagggcatgg gaactgactt ccatggctac
     1981 atgggtgaga ccgcggtcgg ccatggtgtg caaaagcgct tgcaaatcac aagcctgcgg
     2041 ggtggtgtgg tgcacttcca ggtattcgct cttgcctttg aaaaccggtc taccgtcttc
     2101 ttcttcatag atgggcagtc gggcaccgag ggtacctacc aggccgcatt tgtgaccgtg
     2161 gaagttgaga atgtgctcaa ttaaatgtgt ggtcgtggtt ttgccgttgg tgcccgtgac
     2221 gcccaatagc cgcagtctg
//
LOCUS       DAOQNZ010000663         2236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30492_length_2236_cov_3.090784, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000663 DAOQNZ010000000
VERSION     DAOQNZ010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30492_length_2236_cov_3.090784"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKW73_11835"
     CDS             complement(<1..392)
                     /locus_tag="PKW73_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74026.1"
                     /translation="MTFSQAPVSQARTGPYQPPDLLIFFVNSLQPLPEFFDMPMPADC
                     IVELPAKRDALRRAHSLALQEEGSRQNRRDELVIELEPGAGDDSERAQACLRQTLKAI
                     KTVGPARLFILERDSLALEAKNMLEPLTA"
     gene            complement(403..2013)
                     /locus_tag="PKW73_11840"
     CDS             complement(403..2013)
                     /locus_tag="PKW73_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74027.1"
                     /translation="MFKGEKSDKQELKVTNDGTIEYKRSNGNLVKEGLTRTTEVDPDG
                     RLLQVSFPSGAKREFTYDGDKLKTIKESFTTGSGGAEKQRSYTRQGTSNVFTLDECGQ
                     TGSQAHEGSVKAKERIIENLPDAAGNYQYKNKNQEQTRTARSEDLEKIATGELTLSSD
                     NLQEAKEDLLNAFRSQKMNTERADRFVQDLEKNSSKWGVKEENLVKSLDNIRALLDPE
                     LKSPHYSREQLNQLAETALHNIGNPMQIDQGAHPTCNITTVEIFTATRHPEAYTQLVR
                     DVAKTGKWTNAAGETCTPPREALKPGDDEKAYNIDKPSDKLRNISSQLVQMSLINAMY
                     ETGRMDKESTVNGQKQVESRRDWNYVMVPSQKVTSNEYHNGQLVQVTRTVGEDRLRNG
                     KGQYVGKEDSGPNLTVEDNINASKMLLKNPMPYIAGPYKLENQPWVYDLPDAQRLIKA
                     KQEGMLPMGVPTIGGAHVQTIHDVGKNAAGDTIILLDNQHGERMDGWVTIPELHRTIK
                     EKVELTPSINRFGKPFPSDTKENSRPSY"
BASE COUNT          348 a          580 c          656 g          652 t
ORIGIN      
        1 gccgtcagtg gctcaagcat attcttggct tccagcgcga gactgtccct ttccagaatg
       61 aagagacggg ctggtcctac ggttttgatt gctttcaggg tttgccgcag gcaggcttgg
      121 gctcgctcgg agtcgtcccc ggcaccaggt tccagctcaa taacgagttc gtcgcgacgg
      181 ttctggcggc ttccttcttc ctgcaaggcc aggctgtggg cccggcgcag ggcgtctctt
      241 ttggcgggca gttcaacgat gcagtcagcc ggcatgggca tgtcgaagaa ctcgggtagc
      301 ggctgcaggc tattgacgaa gaagatgaga aggtccggtg gctggtaagg accagttctt
      361 gcctgcgaaa ccggcgcttg gctgaaagtc atggcactgt cctcaatagc tggggcgtga
      421 gttttctttg gtatcactgg ggaagggttt gccgaagcgg ttgattgatg gtgtcaactc
      481 cactttctct ttgatggtgc ggtgcagttc gggaatggtt acccaaccgt ccattctttc
      541 accgtgctgg ttgtcgagca ggatgatggt atcaccggct gcgtttttgc caacgtcgtg
      601 aatggtttgc acatgagcac cgccgatggt gggtactccc atgggcagca tgccctcttg
      661 cttggccttg atcagtcttt gcgcatcggg tagatcatag acccagggct gattttccag
      721 cttgtagggg ccggctatat agggcatggg atttttcagg agcatcttgg aggcattgat
      781 gttgtcctcc acggtcaggt tgggaccgct gtcttccttg ccaacgtatt gtcctttgcc
      841 gttgcgcagg cggtcttcgc ctacggttct ggtcacctgc accaactgtc cgttgtgata
      901 ctcgttgctg gtgaccttct gggaaggcac catcacataa ttccaatcgc gtctcgattc
      961 aacctgcttt tgcccgttca cggtgctctc tttgtccatg cgaccggttt catacatggc
     1021 gttgatcagg ctcatttgca caagttgact ggatatattg cgcagtttat cgcttggttt
     1081 gtcgatgttg tatgctttct catcgtctcc cggcttgagg gcctccctgg gaggcgtgca
     1141 ggtttcaccg gcagcattgg tccacttgcc ggttttagct acgtctctga ccaactgggt
     1201 gtaagcttcc ggatgcctgg tggcagtgaa gatctcgacg gtggttatgt tacaagtggg
     1261 atgggcaccc tggtcgattt gcatgggatt gcctatgttg tgcagggcgg tttccgccag
     1321 ttggtttagt tgctcccggg aatagtgagg gcttttcagt tccgggtcca gaagggcccg
     1381 aatattgtcg agggatttga ccagattctc ttccttgacg ccccacttgg atgagttctt
     1441 ctccagatcc tggacgaaac gatctgctct ttccgtgttc attttttgcg accggaatgc
     1501 gttcaggagg tcctccttgg cctcctgcaa attgtccgag ctgagggtca gctcgccggt
     1561 ggcaatcttc tccagatctt cgcttctggc tgtgcgagtt tgctcctggt tcttgttttt
     1621 atactgatag ttgccggcgg cgtccggtag gttttcgatg attctctctt tcgccttgac
     1681 gctgccttca tgggcttggg agccggtttg accgcactcg tccagggtga agacattact
     1741 tgtgccctgg cgcgtgtagc ttcgctgctt ttctgccccg ccactgcctg tggtgaaact
     1801 ttccttaatg gtttttagct tgtctccgtc gtaggtaaac tcacgtttgg cgcctgaggg
     1861 aaagctaact tgcaggaggc gaccatcggg gtcaacttcg gtggttctgg tcagtccttc
     1921 tttcaccagg ttgccgttgc tccgtttgta ttctatggtg ccgtcattgg ttactttcaa
     1981 ctcttgtttg tctgactttt ctcccttgaa caagatggtc tcaagcttgc ctttgtctcc
     2041 cttgcctcct ttgtgctcgg tggtgactgc tgcgatttct ccgtccgggg gtgtcgtacg
     2101 agtgggcggt gtcggcgcgc gaacgcatca tggccgagac ggacgccatg ctgcgcgagc
     2161 tctccgagca ctacgagaag tcgggtgaac tcgcgaccgc gttcgagtac gcgttccgcc
     2221 gggttcgcat cgatcc
//
LOCUS       DAOQNZ010000664         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30540_length_2234_cov_3.546581, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000664 DAOQNZ010000000
VERSION     DAOQNZ010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30540_length_2234_cov_3.546581"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKW73_11845"
     CDS             <1..282
                     /locus_tag="PKW73_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74028.1"
                     /translation="VGPAYDSAELAEMKVIASFCASGIGYNLSRKTDRHYSHLVENAK
                     EYEVVLVLKEGAGGLDDGGTGNTVTNYIAMRRQDLIEKRFDRAWLFSAT"
     gene            complement(275..1414)
                     /locus_tag="PKW73_11850"
     CDS             complement(275..1414)
                     /locus_tag="PKW73_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNA74029.1"
                     /translation="MGSSDKAFRKTASGFALTLALLGTAFPASSAASTVETESKQARS
                     ILHHSDLTSSLRLVPGMLVTVHTFDGLDTHWQTVGDYDFIARVAECESKGKGFIYDWQ
                     MSSPADASGSRKVEDVDVRHSHKVSLFYPKHEVCTLVGYTNVLRVSDDLYNDLKAGRT
                     SDFMIDGPESIVINHGEVTPLPRKISPAGVENVKIRVEGQDRELRAIKTVCDNGWNYW
                     VLDNPQCPMLIQGNAPFRWVVSLGKVDGDGAGDANQEARNIYDQLKRNGIATSYLILF
                     DFDSDRLRPHSKEILLSLSRYLKKDKALKLQVEGHTCTIGGYDYNMQLSRKRAQSVRR
                     YLTESCSIEAERLKAVGLGYTKPEKSNATSAGRERNRRVVFREIK"
     gene            1670..>2234
                     /locus_tag="PKW73_11855"
     CDS             1670..>2234
                     /locus_tag="PKW73_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNA74030.1"
                     /translation="MSKGTGKSVGIDLGTTNSVVAVMEGGQPTVIANAEGARTTPSVV
                     AISKGGERLVGQLARRQAVINPQNTVYSIKRFMGRHFQEVDSEKQIVSYKVKESPEGG
                     CKVPMGGKEYSPEELSAMILRKLKEDAEKYLGEKVSSAVITVPAYFNDSQRQATKNAG
                     AIAGLEVLRIINEPTAAALAYGLEKKKN"
BASE COUNT          580 a          569 c          600 g          485 t
ORIGIN      
        1 gtcggtccgg cctacgatag tgcagaacta gccgagatga aagtaattgc ttccttttgt
       61 gcttcgggca taggttacaa cctcagtcgc aaaacagacc ggcactacag ccacctggtc
      121 gaaaacgcga aagaatacga ggtggtgctg gtgctcaaag agggcgccgg aggactcgat
      181 gatggtggca ccggcaatac cgttaccaat tacatagcca tgcggcggca agacctcata
      241 gagaagcgct tcgaccgtgc ctggctcttt tcagctactt aatttcacgg aagacaacgc
      301 gacgattgcg ctcccgcccg gctgaggtgg cgttggattt ctccggcttg gtatagccaa
      361 gtccgacagc cttcaaacgt tccgcctcaa tcgaacaaga ttccgtcagg tagcgacgca
      421 cagactgagc ccgttttctg gaaagctgca tattgtaatc gtaaccacca atggtgcaag
      481 tatgaccttc cacctgcagt ttgagggctt tatctttctt gaggtagcgg ctgagagaga
      541 gcaatatttc ttttgaatgc ggacgcaatc tatcgctatc aaaatcaaag agaatcagat
      601 aactcgtggc gatgccattg cgcttcaact gatcgtagat attacgggct tcttgattgg
      661 catcgccggc tccgtctcca tccaccttcc cgagggaaac cacccagcga aagggggcat
      721 taccttgaat caacatgggg cactggggat tatccagaac ccagtaattc cagccattgt
      781 cgcagactgt cttgatagcc cgcagttccc ggtcctgccc ttccaccctg attttgacgt
      841 tttcaacacc ggcagggcta attttgcggg gcaagggcgt tacctcgccg tggttaatga
      901 cgatactttc gggaccatca atcatgaaat cggaggtacg tccggctttc agatcgttat
      961 ataaatcatc ggaaacgcgc aatacgttag tatagccaac cagggtgcag acttcgtgct
     1021 taggatagaa caaggaaacc ttgtgggaat gtctgacatc gacatcctcc accttgcgtg
     1081 acccgctggc atcggcggga gagctcattt gccagtcata tataaagcct ttgcccttgc
     1141 tctcgcattc cgccacccgg gcgatgaaat cgtagtcgcc cacggtctgc cagtgggtat
     1201 caaggccgtc aaaggtgtga actgtaacga gcatgcccgg caccaggcgc aaactggaag
     1261 taaggtcgct atgatggaga atggagcgcg cctgtttaga ctcggtctcc accgtactgg
     1321 cggcgctaga ggcaggaaag gcggtgccga gaagcgccag ggtcagggca aagcccgagg
     1381 ccgtcttccg aaatgcttta tctgaggaac ccattccata tccctatata tgatcgacac
     1441 ccagtgtgct cgactaaaga tccgaacttt gtttaagtgt ttgaattatc gcaagagggg
     1501 cgcccaggca cattatagaa agatgcactt agcgcctccg gaagagccag atgattaaag
     1561 aaagctacag tttccgtgaa agaggcgcca ttgttaatag attgaattta gaagctgccc
     1621 accggggcaa gcgaagtatt accggtttgc aatattagag aaatacatta tgtcgaaggg
     1681 aactggcaaa tctgtgggca ttgacctcgg tacgacgaac tcagtcgtgg ctgtaatgga
     1741 aggcggtcaa ccgacggtaa tcgcaaacgc tgaaggagcg agaaccacac cttcggttgt
     1801 agccatatcg aaaggcggcg aaagacttgt gggacaactg gccagacgcc aggccgtcat
     1861 taacccgcaa aataccgtct attcaattaa gcgctttatg ggaagacact tccaggaagt
     1921 agattccgaa aagcaaattg tctcttataa ggttaaggaa agtcccgagg gcggctgcaa
     1981 ggtgcccatg ggcggcaagg aatactcgcc ggaagaactc tcggccatga ttctgcgcaa
     2041 actgaaggaa gacgccgaga agtatctggg cgagaaagtc agcagcgccg ttatcacggt
     2101 accggcttac ttcaacgatt cccagcgcca ggccaccaag aatgccggtg ctatcgccgg
     2161 tctcgaagtg ctgcgcatca tcaacgaacc aacggcggcg gctctggcct atggtctgga
     2221 gaagaagaaa aacg
//
LOCUS       DAOQNZ010000665         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30541_length_2234_cov_3.388251, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000665 DAOQNZ010000000
VERSION     DAOQNZ010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30541_length_2234_cov_3.388251"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            510..1355
                     /locus_tag="PKW73_11860"
     CDS             510..1355
                     /locus_tag="PKW73_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74031.1"
                     /translation="MDRTLFSPTMLEAFRACKRAYFLAYHRDGAEQARAQASSICKRF
                     ILRGLAEINRARLSSVSQIQKFMGESWPVERLSGDPDLKDAATRAFLFTYKTLLRYQS
                     QPYLPKGAQVEGVSTRVRSRVQNERVYLEEVFDLVLWYPEERKLELVIFNLRPVKEIN
                     PAWPSATHLVRQHLAERLRVRLPYERLVLTNIKVGPQEMKITSREASSEMYRLHWPEI
                     VKSLSEMKEMADMPEHDGVHAKEGCSYCRAIESRMAAEVISVREVTEEDALGGEISGK
                     YPLSA"
     gene            1532..1978
                     /locus_tag="PKW73_11865"
     CDS             1532..1978
                     /locus_tag="PKW73_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNA74032.1"
                     /translation="MDKQKVLDALNLDLEHEMSAMIRYLHHSFIVTGPLRGSLTALFR
                     TMATDSMNHAIKLGEKIVALGGHPSVKIQEIYEPGEQSIEEMLKENIEAEKMHLALYE
                     EQLDIMHDNTPLRLMMEQVIVDEARHIEELEMYVRNNTPAKTKAKV"
BASE COUNT          576 a          502 c          594 g          562 t
ORIGIN      
        1 cagaattgaa ttgaagcaga ttgggagtgc tggatatcag ctcagctaac attatcaatt
       61 aatctcaatc tttaatgtgt cgttgtgagc ctgccaagtc tttgatttta tctgtgatgg
      121 atattttgca ggtagacttt gggtgatctt tcgcgaccct ttcggatgtg ttttgtatgc
      181 agtaaggagc cgggttttgc cttgttgtca tgtggattcg gcggaaacaa ggatgggcat
      241 ttataagtca gtgagcttat cggtgcaaaa tcgccgaacc aaaagataat tgctcgctgc
      301 aagcaaatca tatttataga gtccaaagtg caaatttctc aaactgcttt ggcaagcgtc
      361 tgcgtactgt tttcgccaac gcttgcggcg gattgcctgg cttttatggt tggcaacagg
      421 gctgcctgga atttatttga cgacggcgct ttggatacta taaattggta tgacatacaa
      481 aaatatggta acaccagggg ggtctcatca tggaccgtac attgttttcg ccaactatgc
      541 tggaagcctt tcgtgcctgc aaacgggctt acttcctggc ctaccatcgc gatggcgccg
      601 agcaggctcg ggctcaggcc tccagcatct gtaaaaggtt catattgagg gggttggccg
      661 agatcaatcg cgccaggttg agcagcgtca gccagattca gaagtttatg ggtgagtcct
      721 ggccggtgga gcgtctgagt ggtgatcccg acctgaaaga cgccgccaca cgagcctttc
      781 ttttcactta caaaaccctg ctgcgctacc agagtcaacc ttatttaccc aaaggtgctc
      841 aggtcgaggg tgtttcaact agagtgcgtt cccgtgttca aaacgagaga gtctacctgg
      901 aagaagtatt tgatcttgtg ctctggtatc cggaagaacg caagctggag ctggttatat
      961 tcaatttgcg accagtaaaa gaaatcaatc cggcctggcc ctccgccact catttggttc
     1021 ggcagcacct ggctgagagg ctcagggtgc gcttgcctta tgaaagactg gttttgacaa
     1081 atatcaaagt aggccctcag gaaatgaaga taacaagccg agaagccagc agtgagatgt
     1141 accgcttgca ctggcctgaa atagtcaaga gtctttccga aatgaaagag atggctgata
     1201 tgccggagca cgatggcgtg cacgcgaaag aggggtgctc ctactgccgg gccatagaga
     1261 gccggatggc tgctgaagtt atatcggtaa gggaagtgac agaggaagat gctctcggcg
     1321 gcgaaataag cggcaaatac ccactttccg cttgataaca ttggcgctcc aataatatag
     1381 ctataatttt gcggttgcac ctggaggcaa agaccgttct gtctttggcc ctgtgcgttg
     1441 ccggcagaaa atcaggcgct taagacgact ggctttcact tctatttttg taagtgaatt
     1501 ggctctcgct gggagccggg ggaagaaaat aatggacaag cagaaagtac tggatgcgct
     1561 caatctcgat ctcgagcacg aaatgtcggc catgatccga tatctacacc actcctttat
     1621 tgtgacaggg cccctgcgcg gttccctcac ggcactcttt cgcacgatgg ctaccgactc
     1681 catgaatcat gccatcaaac ttggtgagaa gattgttgct ctgggcggac atccttccgt
     1741 aaaaattcag gaaatatatg agcctggtga gcagagtatc gaagagatgc tcaaggagaa
     1801 tatcgaagct gagaagatgc acctggcgct atatgaagag cagcttgaca taatgcacga
     1861 caatacacct ttgcgcttga tgatggagca ggtgattgtg gatgaagctc gtcacattga
     1921 agaactggaa atgtacgtcc gcaataacac cccggctaaa accaaagcaa aagtctgaag
     1981 ctaaatccat agctatacag atacagtcga gaagtaaaaa aggaaaaggg cggattaccc
     2041 gcccttttcc tttcttgctt ctgggggatg gtagtgtcac cactggtgaa ggcagtgtcg
     2101 aatgcgcttt ccctgagcat acacaagcta ctagttcgat ctgcgaagaa tatgtggcac
     2161 gtgtcgccag ggatatctaa ctgtggcttc tctcggttga caggttccac gccaaggtag
     2221 gccactttcg cagc
//
LOCUS       DAOQNZ010000666         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30589_length_2232_cov_4.118512, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000666 DAOQNZ010000000
VERSION     DAOQNZ010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30589_length_2232_cov_4.118512"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PKW73_11870"
     CDS             <1..909
                     /locus_tag="PKW73_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HNA74033.1"
                     /translation="DLTAYGGDIKRALGKKLPPLFRGYPTGFTVAPVGTQSAPLLDYA
                     EAREEPVEHRVSVTSIADFIAQYLGFDLSQEIRSLDWLTFPEQKLLSLTGGRLFHDQI
                     GIKKVLSRFAYYPQDVWLYLLACAWRRIEQEEHLMGRAGSVGDELGSRLIASRLVRDL
                     MRLSFLMEKQYAPYPKWFGTAFGKLALAPELKPLLEQVLSQSVWQEREKFLVKAYELL
                     AQHHRRLGVTEPLPTECSQFHDRPFLVISKGLYSAAILAQIEDPFLQELATRPLIGSV
                     SQFSDSTDLQVDPLWRPALVDLYGKQ"
     gene            complement(960..>2232)
                     /locus_tag="PKW73_11875"
     CDS             complement(960..>2232)
                     /locus_tag="PKW73_11875"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74034.1"
                     /translation="LLKPHDDMVVASLNNLSALYFFQGKHDQAEAPCKKLCEVYEAVL
                     GKDHAEVATSVNNLALIYSAQGKNEEAQELFARALAIKEKALGRQHPEVGDILNNMAN
                     ICISETRYEEAEPLLTEAIAILETGIGSDHPDLIPILTSLAAVSQVNGNHKAASKHLE
                     RLLSLKEINLAPNHPEVVDCIVNLASSYLYEGRFTDAERLYKRAMGIRERAKGRQSPE
                     VAEIIAAIAFVHQSDNKYLLAENLYKEALAICEQSRGVNSPEAEARLWDLACLYHNNA
                     RYQKAEPLWQRLATLREEHLGENHPDTQACLDSLTICYLQQTKYAEAEKLLVRLKNLR
                     EASLGPDHADVAACLHYLAEIMRHDGRFDQAEQYYKRALDIRTKALGANHPEVAHSLE
                     GYALLLAATYREMEAEHMRVCAEAILKAASH"
BASE COUNT          457 a          580 c          609 g          586 t
ORIGIN      
        1 gatctgacgg cttacggagg ggatatcaag agggcgctgg gcaaaaaact gcccccactt
       61 tttagaggtt atccaaccgg tttcacggta gccccggtcg gtactcaatc cgcgccgctt
      121 ttagattacg ccgaggcaag agaagaaccg gtagaacatc gtgtttccgt tacgagcatt
      181 gccgatttca tcgctcagta cctgggtttt gatttgagtc aggaaatccg gtctctcgat
      241 tggttgacct tccccgagca aaaactgctt tcactcaccg gcggccgtct ctttcatgat
      301 caaatcggca taaagaaagt gctcagccgt ttcgcttatt atccccagga tgtctggctc
      361 tatctgcttg cttgcgcctg gcgtcgcatt gagcaggaag agcatcttat gggccgggcc
      421 ggctctgtcg gcgatgaact ggggtcgcgg cttatagcat ctcgcctggt tcgagatctg
      481 atgcgcctga gcttcctgat ggaaaaacaa tacgcccctt atcccaagtg gtttggcact
      541 gcttttggca aactggccct tgcacctgag cttaagccgc tgcttgagca ggtgctgagc
      601 caatctgtct ggcaggagag ggagaagttt ctcgtcaaag cttatgagct tttagctcag
      661 catcaccgtc gcctgggagt aacggagccc ttgcctactg agtgttcaca gtttcatgac
      721 agaccatttc tggtcatcag taaaggcctt tactctgccg ccatcctcgc ccagatagaa
      781 gatcccttct tgcaagaact tgctactaga ccgctcatag gcagtgtctc acagttcagc
      841 gacagcaccg atctgcaagt cgatcccctc tggcgcccgg ctctggtcga cttgtatggc
      901 aagcagtgac tggtatcgct gctggtggcg tgttcgagga cgttagccta catgctcttt
      961 tagtgactgg ctgctttcaa tatggcttca gcacaaaccc gcatgtgctc tgcttccatc
     1021 tctctataag tggctgccaa aagcagggcg tagccttcca gagagtgagc cacttccgga
     1081 tgattggctc ctagagcctt ggtgcgaata tcgagggcgc gcttgtaata ctgttccgcc
     1141 tgatcgaagc gaccgtcatg gcgcatgatc tcggccaggt aatgcaggca ggcagccaca
     1201 tcggcgtgat cgggaccaag ggaggcttct cgcaggttct tgaggcgcac cagaagtttt
     1261 tcggcttcgg catacttggt ttgctgtaag tagcaaatgg tcaagctgtc gaggcaggcc
     1321 tgggtgtctg gatggttttc acccagatgt tcttccctga gggtggccaa tctctgccag
     1381 agcggctcag ccttctggta gcgggcatta ttgtgataaa ggcaggccag gtcccagagc
     1441 cgtgcttccg cttccggact gttaactccg cgggattgtt cgcaaatggc cagtgcttct
     1501 ttatatagat tctcggccag gaggtacttg ttatccgatt gatggacaaa agcaatggcg
     1561 gcgataattt ccgctacttc cggactctga cgccccttgg ctctttccct gatacccatg
     1621 gctctcttgt agagccgctc tgcgtccgtg aagcggccct catagagata ggatgaggcc
     1681 aggttgacaa tgcaatccac aacttcgggg tgattgggtg ccagatttat ttctttcaag
     1741 gatagtagtc tttccaagtg cttactggcc gccttgtggt tgccgttcac ctgggacacc
     1801 gccgccaggc tggtaagaat tggaatcaaa tccgggtggt cggagcctat accggtctcc
     1861 agaatcgcta tggcttccgt caaaagcggc tcggcttctt catatctggt ttccgatatg
     1921 cagatattgg ccatattgtt gagaatatcg cccacttccg ggtgttgcct acccagagcc
     1981 ttttctttga tagccagggc tctggcgaag agttcctggg cctcctcatt tttgccctga
     2041 gctgaataga tgagggcaag attgtttact gaagtggcca cttcggcatg gtctttaccc
     2101 agaacagctt cgtaaacttc gcaaagcttt ttgcagggcg cttcggcctg gtcgtgtttg
     2161 ccctggaaga aataaagagc ggaaagatta ttgaggctgg ctacaaccat gtcgtcatga
     2221 ggtttgagga gg
//
LOCUS       DAOQNZ010000667         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30653_length_2230_cov_3.521839, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000667 DAOQNZ010000000
VERSION     DAOQNZ010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30653_length_2230_cov_3.521839"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1081)
                     /locus_tag="PKW73_11880"
     CDS             complement(251..1081)
                     /locus_tag="PKW73_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74035.1"
                     /translation="MNRARFNIASWPFRLGLKVVAAILCFGSGFAGPASAGGKGVVKI
                     EQVTAAHGPATVYISDQGIQFHMPRKGLVYYCMAPDWRLVVFKKATNQGLIQRREQWK
                     GNSINKEKIVVLKSRSVPIQFAGKSCRRIFLEVAPLDSYVSKSAAFFRSEGRSSTTFK
                     HMELVESISVKLGPEQLQLARYVYGMPEINGVLLDETLLGSNGSRFNLIKTTSIGTAG
                     PTELSWKYPTGFKLVGWSDLLEQKKDLEAAADILGTLYEDDMDDKKTAPAKTGRGKPK
                     "
     gene            1232..2209
                     /locus_tag="PKW73_11885"
     CDS             1232..2209
                     /locus_tag="PKW73_11885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA74036.1"
                     /translation="MADQQFVVIGAGIIGLTTARVLQQAGHRVQIISAELPLNTTSVA
                     AAAFWYPSDSEPKEDVLRWGLKTHQTFKELKEEGVAGITLERALEVHPDPTWHPWWLN
                     VVANPRKADLAELPPPVAGGSGYWFDTLVIDTTVMLKWLMEQFSAAGGAFVQQKLNNI
                     TEIASQTEIIVNCSGVGARDLVGDSELYATRGQTLKIRRKPEHRAVWEMSTKPLVTHI
                     IPREADTVIGGVFQEHNYSRVVDPLDTEGILQRCRNLQPTLGTLNESDILSVSVGLRP
                     TRARLRLESEKLLSGSTVIHNYGHGSCGWSLSWGCAEAVRGQVQGMLPL"
BASE COUNT          568 a          581 c          559 g          522 t
ORIGIN      
        1 ggctctgcgg cctggccggg attagaagct actgccaaag caaaagtcag caagaagctg
       61 agggtgccaa ccttcagggg ctcctgaaac gcttttacat ggtgtagact atcaggcatt
      121 ctggatatat cattcactcg catcattcgg aaccgtccac tgcaaactta gcagcgcgct
      181 atatatggta ccagcaactc caggtagccc gcttaatttg cccgctccgt caagcatccc
      241 ggtgaatcta ctacttgggc ttccccctgc cggttttagc cggagcggtt ttcttatcat
      301 ccatgtcatc ttcgtaaaga gtgccaagaa tgtcggcggc tgcctccagg tctttcttct
      361 gctccaatag atccgaccag cccaccaatt tgaagcccgt gggatacttc caagaaagct
      421 cggttggacc agccgtcccg atactggtgg tcttgatcaa attgaacctg ctgccattgc
      481 tgccaagcaa ggtttcatca aggagcaccc catttatctc tggcatacca taaacatacc
      541 gggccaattg cagttgctca gggccaagct tgacggaaat cgattcgacc agctccatat
      601 gcttgaaagt tgtggaggag cgcccctcgg agcgaaagaa agccgccgac ttggagacat
      661 atgaatctag cggtgccacc tcaagaaaga ttcgccgaca actcttaccg gcgaactgaa
      721 tgggcaccga acggctcttc agcactacta ttttctcttt gtttatggaa ttacccttcc
      781 actgttcacg acgctggatt aaaccttgat tggtagcctt tttgaagaca acgagcctcc
      841 aatcaggagc catacaataa tagaccagcc ccttgcgcgg catatgaaac tggataccct
      901 gatcggatat ataaacggtg gcgggtccat gggcagcggt cacttgctca attttaacta
      961 cacccttgcc gccggccgat gccggaccgg caaaaccact gccgaagcac aatatagcag
     1021 ctactacctt gagcccaagc ctgaacggcc aactggcgat attgaacctt gccctattca
     1081 tttgatgaac taatcaacga gaaccaatac cggggatact tcgagtttag cacctaaagt
     1141 ttccagctca gtccttctag gggctaacag aactatctca atacccgtta tgcttataaa
     1201 ctcattggac ctagattcag gagataggca aatggctgac cagcaatttg tagtaatcgg
     1261 ggccggcatc atcggcttga ccacagcgcg agtactacag caagccggtc atagagttca
     1321 gatcatttca gccgaactgc cccttaacac tacatcggtt gccgctgcag ccttctggta
     1381 cccttcggac agtgagccaa aagaagatgt attacgctgg ggtctaaaga cacaccagac
     1441 attcaaagag ttgaaggaag aaggggttgc cggaattaca ctcgaacgag cactggaggt
     1501 tcatccagac ccgacatggc atccatggtg gttaaatgtg gtagcaaatc caagaaaggc
     1561 agacttagct gaattgccgc cccctgttgc gggtggtagt ggatactggt ttgacacgct
     1621 ggtcatcgat acaacggtca tgctcaaatg gttgatggaa cagttcagcg ctgccggagg
     1681 agcatttgtt caacagaagt tgaacaacat caccgaaatt gcctcacaaa cagagatcat
     1741 agtcaactgt tccggtgtcg gtgcccgtga tctggtcggc gattccgaac tctatgccac
     1801 cagggggcaa actctaaaga ttcggcgtaa gcctgaacat cgcgcggtct gggaaatgag
     1861 cactaagcct ctggtaacgc atataattcc ccgtgaggca gataccgtta ttggtggcgt
     1921 gtttcaggag cacaattata gtcgcgtcgt cgatcctcta gacaccgaag gaatacttca
     1981 gcgctgtcga aatttgcaac cgactcttgg aactcttaac gagtcggaca tcctgagcgt
     2041 gtctgtgggt ctaagaccta cccgggcgcg actgcgcctg gagtctgaaa aactactttc
     2101 cgggtcaact gtaattcaca attatgggca cggcagttgt ggctggagtt tatcctgggg
     2161 ttgcgccgag gctgtccggg ggcaagttca gggcatgctg cctttgtaga tgaaggcgag
     2221 agtatatggg
//
LOCUS       DAOQNZ010000668         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30671_length_2229_cov_3.907544, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000668 DAOQNZ010000000
VERSION     DAOQNZ010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30671_length_2229_cov_3.907544"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="PKW73_11890"
     CDS             complement(<1..1697)
                     /locus_tag="PKW73_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNA74037.1"
                     /translation="MKHNSRHNGRHPRVIEVRTAAVEPDVSTLSVDEVLTRLNTTVDG
                     LSEKSAAERLQRHGRNSISVEVPIAVGALLRENFLHTMALLLWAAGLIGFLARMPQLG
                     VAIWLVNIINGAFSFWQEFRAQKTTAALKQLLPLSVQVRREGKVRTILAYELVPGDVV
                     IVNEGDKISADARVIEDESLTVDQSTLTGETTRLVKTAALFSKRMGKDANLIRADLPN
                     LLFAGTSCLSGHGLAVVYATGTHTEFGRIARLTATIAEQPSPLQREMERVTKIVSILA
                     VSIGALMFVIAITMAHVRPEAAFIFAMGMVVAFVPEGMVPTITLALALAVQRMVKRNA
                     LVKRLSSVESLGCTNVICTDKTGTLTENRMTVARVMAGGKDYLFSGHGYELNGSLFSS
                     GNEPVSELPPELAALLQVGLLCNNATLVKGADGLSTSGDPTELALLVAAGKAGLFQSD
                     AAATSKRLHENAFDSHRKRMSTIYEVNVGAEPGSLERIAFIKGSPAGVLEHSTDWLSP
                     LGLQTIDEERRQGILQEIDGYAGQGLRVIAVARKTLNDQSALDIAGVESNLTFLGLFA
                     "
BASE COUNT          553 a          643 c          561 g          472 t
ORIGIN      
        1 gcaaagagac cgaggaaggt aagattcgat tccaccccgg caatgtcgag ggcagactga
       61 tcattgagag ttttgcgagc tacggcaatc actctcaatc cctgaccggc gtagccgtcg
      121 atttcttgca aaataccctg gcgccgctct tcatcaatgg tctgcaagcc caagggcgac
      181 aaccagtccg tgctatgctc cagtacaccg gccggcgaac cttttataaa agcgatccgc
      241 tcaagggaac caggctcggc accgacattt acttcataga tagtgctcat gcgctttcta
      301 tgagagtcaa aggcattttc atgcagcctt ttgcttgtgg cggcggcatc cgactggaac
      361 agtccggcct tgcctgcagc cacaagcagc gctaattccg tcggatcacc ggaggtgctc
      421 agaccatcgg cacctttcac cagggtggca ttattacaaa gcaagccaac ttgcaagagc
      481 gccgccagct caggcggcag ttcgctaacc ggttcattac ctgaagagaa gagactgccg
      541 ttcaattcat agccatgacc tgaaaataga tagtccttgc cgcccgccat caccctggcc
      601 acagtcatac gattttccgt aagcgtaccg gttttatcag tgcaaattac gtttgtacaa
      661 ccaagagatt caactgagga aagccttttc acaagcgcat tgcgcttaac catacgctgc
      721 acggccagag ccagggccag ggttatggta ggcaccatac cctccggcac aaaagccacc
      781 accatgccca tggcaaaaat gaaagccgct tccggcctca catgggccat ggtgatggca
      841 atgacaaaca tgagggcgcc gatactaacg gccagtatcg aaactatttt agttacccgc
      901 tccatttccc tctgcaaggg gcttggctgt tcggcgattg tggctgtaag cctggctatg
      961 cgtccgaatt cggtatgagt gccggtggcg taaaccacag ccagtccgtg accggacagg
     1021 caggatgtac cggcaaaaag caaattcggc aggtccgcac gaataagatt ggcatccttg
     1081 cccatgcgct tggaaaacag agcagcggtc ttgaccagcc tcgtggtctc gccggtgaga
     1141 gtggactggt ccacggtgag cgactcatcc tcaatcaccc tggcatcggc ggaaattttg
     1201 tcgccctcgt tgacaatcac tacatcgccg ggcaccagct cataagccaa gatggtgcga
     1261 accttaccct cccgccgcac ctgcacgctc aggggcaaaa gctgcttgag ggcagcggta
     1321 gttttctggg ctcgaaactc ctgccagaag ctgaaggccc cattgatgat atttaccagc
     1381 cagatagcca cgcccagttg cggcatccgg gccagaaaac caatcagtcc ggcagcccag
     1441 agcagtaaag ccatggtgtg caggaaattt tcacgcagca acgcgccaac ggcaatgggc
     1501 acttccacac tgatggaatt gcggccgtgc ctttgcaacc gctccgccgc cgacttctcg
     1561 ctcaatccat ctactgtggt gtttaaccgg gtgagaacct catccaccga caatgtggag
     1621 acatcgggtt ccacggcagc ggtgcgcacc tctattacgc gaggatgtcg cccattatgc
     1681 cttgaattgt gtttcatcaa gccgctctaa ttctcatgga gcagtttcaa ggtttcccgg
     1741 gtgtcaggca ccaggagcct ttcccgctta cgcaaaccac gcaaagttcg cgggattgag
     1801 aatagcactt tttacacacc ttcttgtgac agttcttgca tgaaacaaga ccatccccga
     1861 gtaccggcaa aagcttggga tcgctcaact tgccgcaaac gccgcaagct cctgtaattg
     1921 gattttgagg aagcattgac atctcaccct cgaaggcagc tatcggttaa atcacgccgg
     1981 tcgggaagaa atcatccaac caggagtctc aaactgccca ccagcatact aaaatcataa
     2041 ccggccgggc tcagggttgc caggataccg gtctttgtca ccgaaccatt cagtacggga
     2101 cggtagctca ttggcaatgc accagggcca tcagtatagc cttcaatcag cggcgggtaa
     2161 gatgaaaaat tatattctca gtatcgacca gggcaccaca agctgccggg ccataatctt
     2221 tgataagag
//
LOCUS       DAOQNZ010000669         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30708_length_2228_cov_3.369995, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000669 DAOQNZ010000000
VERSION     DAOQNZ010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30708_length_2228_cov_3.369995"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKW73_11895"
     CDS             <1..1096
                     /locus_tag="PKW73_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74038.1"
                     /translation="PVSNSRMAVKTIRITKLIARLITTLTVLTCSLLMAPQAIAGQDY
                     DTALNYYQQKQYEKAAVHFETASLRAPGNISANYYAAYCFHLVGKRQEAIQSYRRLCK
                     NFPGTREAQQAQRALSAIDPQYKDGQAGNPAAESGNTGNLNVGGRKKETAENLTNRLV
                     KVQKATGKLADVTGDFIKSIKEMVAAIPLPILVLMDERGGEFDIAPSVVEHDLRIQNT
                     APRGWGSESSWKESPAVCSGTKVIIAQYKLNSSTGEYVTTETEIGVVRHETGHAIDHC
                     LNGFSTRESFRHAYYLDEARVPDELRGRLAYFLQKSTSGPSEVFAELICSRLGGETDN
                     YRKESGDLVYKHFPLCRKEMENLLAELGVK"
     gene            complement(1099..2067)
                     /locus_tag="PKW73_11900"
     CDS             complement(1099..2067)
                     /locus_tag="PKW73_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74039.1"
                     /translation="MLRGRVLSLVTICAVCGLMLPVVSVLPVCQLSPVSPLSPVSPVS
                     PVPFVPFVPFVPFAASAAELTYPTDYLSALRQYQSARNLYQRHRQIFQSNINTQGSLN
                     VSVESSLKLGNKPNFKMPFIDLPDDCPFLDVAHRDYEITQMEESLAQLIPALFASRDK
                     ISRGEFRATWQDLMQRSLNVEKAVASLNHDLAVLSASNSRKITEMIHAADRDGIQSEY
                     QSTYGIFNATNRVENELFRRADKHGQIALSLKSMLLASPPAAMADPVSAYGATGDGAQ
                     SGASVPVPIDPQFSGQDIQAQQRQLDSEYQEVMRLYQVLSSMGRVP"
BASE COUNT          606 a          585 c          594 g          443 t
ORIGIN      
        1 accggtttcg aattcccgaa tggcagtgaa aacaatcaga atcactaaac taatagcaag
       61 acttataaca actctcaccg tgcttacctg ctcactgctc atggcaccgc aggccatcgc
      121 cggtcaggat tacgatacag cgctaaatta ctaccaacaa aagcaatacg agaaggccgc
      181 cgtccatttc gaaacagcct ccctgagagc accgggcaac atctcagcca attactacgc
      241 agcttattgc ttccacctgg tggggaaaag gcaggaggcc atccagagct accggcgcct
      301 gtgcaagaat tttcccggca ccagggaagc tcagcaggcc caaagagcgc tgtctgccat
      361 agatccccag tacaaggacg gtcaggcggg aaatccggct gctgagagcg gcaacacggg
      421 aaacctaaac gtcggcggca ggaaaaagga aactgctgaa aatcttacca accgcctggt
      481 caaggtacaa aaggccacgg gtaagctggc agatgttaca ggcgatttca tcaagagcat
      541 caaagagatg gtggcggcaa ttcctctgcc gattcttgtt ttgatggacg aaaggggcgg
      601 ggaattcgat atagcaccgt cggtggtgga acacgatctg cgcatacaaa acacagcccc
      661 cagaggctgg ggcagcgagt caagctggaa agagtcgccg gcagtctgct ccggcacaaa
      721 agtgatcatc gcccagtata aacttaacag cagcaccggc gagtatgtca ccactgaaac
      781 tgagataggc gtagtcagac acgaaaccgg acatgccatc gatcattgcc tgaatggttt
      841 cagcactaga gaatccttca gacacgccta ctatctcgat gaagccaggg taccggatga
      901 actgcgtgga aggctggctt attttttaca gaaatccacc tccggccctt cagaggtctt
      961 cgccgaactc atttgttcgc gcctgggcgg tgagaccgac aactatcgca aagagtcagg
     1021 agaccttgtc tacaagcact tccccctatg ccgcaaagaa atggagaatc tgctggcaga
     1081 actgggagtc aaataaattc agggcactcg ccccatggac gacaaaacct gatagagacg
     1141 cattacctcc tggtattcgc tgtctagttg cctctgttgg gcttgaatat cctgaccgga
     1201 aaactgcgga tcaataggca ccggcaccga tgccccggac tgcgcaccgt caccagtggc
     1261 gccataggca gaaacagggt cggccatcgc cgcaggcgga ctggcaagca acatgctctt
     1321 gagggaaagg gctatctgcc catgcttatc agcccgccgg aagagctcat tttcaacgcg
     1381 gttggtggca ttgaaaatgc cataggtgga ctgatattca gactgtatgc cgtctctgtc
     1441 ggcggcatgg atcatctctg taatcttacg gctattggag gcgctcagta ccgccagatc
     1501 gtgattgaga gaagccaccg ccttctcaac attgagagaa cgttgcatca gatcttgcca
     1561 ggtagcacga aactcgccgc gggaaatctt gtctcgactg gcaaagaggg ccggaataag
     1621 ctgggcgaga gactcttcca tctgggttat ctcgtaatcg cggtgagcta catccagaaa
     1681 gggacaatca tcaggcaaat ctatgaacgg catcttgaag ttgggtttat ttcccagttt
     1741 gagagagctt tccactgata cgttgagtga accctgagta ttgatattgc tttgaaaaat
     1801 ctgccggtgc cgctgataga gattcctggc gctttgatat tgcctgaggg cgctcaggta
     1861 gtcggtggga taagtgagtt ctgcggcgct ggcggcaaaa ggtacaaaag gtacaaaagg
     1921 tacaaaaggt acaggagata caggagacac aggagacaaa ggagatacag gagatagctg
     1981 acataccggc agtaccgaga ctaccggcag catcaaaccg cagacagcac aaatggtgac
     2041 aagagagagc accctgcccc tgagcaaacg gcccttagat atccacaccg tcttcatgta
     2101 aaaccgacct agaacccact gtccgagccc gatgcccctt cgctcattgt aggcacggtt
     2161 tcagttccct gcgggggtgc caccacttga ccgctatcat agctggaata agaggcagcg
     2221 gcaccctg
//
LOCUS       DAOQNZ010000670         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30740_length_2227_cov_2.893186, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000670 DAOQNZ010000000
VERSION     DAOQNZ010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30740_length_2227_cov_2.893186"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKW73_11905"
     CDS             <1..601
                     /locus_tag="PKW73_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNA74040.1"
                     /translation="EIVHSEEWKMSEPANSTLCPRLGAAQVAALPDAVLIDVRSPIEF
                     ECEHIEGAINIPLESLESRCEEVPRKGQLVMVCRSGKRAERGAYTLMGRAFQPQVLEG
                     GMLAWRQAGMPVKEGKKRLSIERQIQLVVGTGVLTGVILGAFVNPLFLIIPGFFGAGL
                     TFAGLSGTCALGILLGKAPWNKLESPGNAKACNKSSCCN"
     gene            616..1362
                     /locus_tag="PKW73_11910"
     CDS             616..1362
                     /locus_tag="PKW73_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA74041.1"
                     /translation="MLHMLIAASSGIATGSLLGLLGGGGSIIAVPVLVYLLGFDAKTA
                     VASSLLVVAFASLLAAYSHFRHKAVVVSTALVFGGAGGIGSLAGAQLAGLLSDSVQLT
                     AFALVMALVALLMLRAKPPDIQSDTRSAPLALVLTAGVGAGILTGLLGVGGGFVIVPA
                     LTLLLKLPITKAIGTSLLIIAMNSAVGAVYYASRLHFDASILPFALGALAAAPIAGRL
                     AHRIAQERLKVSFAISLLLLSGWMLFKEVH"
     gene            1378..>2227
                     /locus_tag="PKW73_11915"
     CDS             1378..>2227
                     /locus_tag="PKW73_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA74042.1"
                     /translation="MYFQQFYLGCLAHGSYLIGSEGEAAVIDPQRDVDQYIEAADKAG
                     LTIKYIFETHLHADFVSGHKELSERTGADIVFGAKAGAAFPHIAAHDGDIFRVGEVCL
                     TILETPGHTPESICILAKDTASSKPEKLFSGDTLFIGDVGRPDLVGSKGYSAEEMAAL
                     MYDSLHEKLLNLPDDVEVYPAHGAGSLCGKQLSDERSSSIGQQKKFNYALQPMSKEAF
                     VEVLTADQPEVPAYFPKDVELNRLGAVPLKELALPRAMTASETLQYLEKGAVVLDVRS
                     AAEYSAN"
BASE COUNT          443 a          568 c          619 g          597 t
ORIGIN      
        1 tgaaattgta cacagcgagg aatggaaaat gtcggaacca gccaattcaa cactttgtcc
       61 ccggcttggt gctgcgcaag tggcggcttt gcccgatgcc gtgcttattg atgtgcgtag
      121 cccgatcgaa tttgagtgcg agcacatcga gggggctata aatatacctc tggaaagcct
      181 ggagtctcgt tgcgaggaag tgccgcgcaa ggggcaactg gtaatggttt gtcgcagcgg
      241 taagcgcgcc gagcgcggcg cctatacgct aatgggcagg gcctttcagc ctcaagtcct
      301 ggaaggcgga atgctcgcct ggcgtcaagc cggcatgcct gtcaaggaag gcaaaaagag
      361 gctttccatc gaaaggcaga ttcaacttgt ggttggcacc ggtgttctca ccggcgtcat
      421 tctgggcgct tttgttaatc ctcttttcct cattattcct ggtttcttcg gagccggtct
      481 gacttttgcc ggcttgtccg gaacctgcgc tctcggcatt cttcttggaa aagcaccctg
      541 gaataagctg gaaagtcccg gcaacgcaaa agcatgcaat aagagtagct gctgtaattg
      601 aggtggtagg tgactatgct gcacatgcta atcgctgctt cctccggtat cgctaccggt
      661 tctctgcttg gactgctggg cggcggtgga agcatcattg cagtacctgt cttagtctac
      721 ttgcttggtt ttgatgccaa gactgctgta gcttccagct tgcttgttgt cgcttttgcc
      781 agtttgcttg ccgcttacag tcatttccgc cacaaggcgg tagtggtatc gacagccctt
      841 gtttttggtg gtgccggcgg tatcggcagt ctggctggtg ctcaactagc cggtttactc
      901 tctgattcgg ttcaacttac ggcgtttgct ctggtaatgg ccctggtggc gcttttgatg
      961 ctgagagcaa aacctccgga tattcagtct gacacaaggt ctgctcctct agcgctggtg
     1021 cttacggccg gcgtcggtgc cggcattctt acgggacttc ttggtgtggg cggcgggttt
     1081 gtcatcgtgc cggccttaac attgttgctc aagcttccta tcactaaggc tattggaact
     1141 tccttattga taattgccat gaacagtgcc gtcggtgccg tctattatgc ctctcgtctg
     1201 cattttgatg cttccatctt accctttgcc ctgggcgccc tggccgcagc accaatcgcc
     1261 ggccgtctgg ctcatcggat tgctcaggaa agacttaaag tcagttttgc tatttcacta
     1321 cttctcttat ccggctggat gctctttaag gaagtgcact aaattctgga ggcgtatatg
     1381 tattttcaac agttttacct gggatgcctg gcccacggct cttacttgat tggctccgaa
     1441 ggcgaggctg cagtcatcga tcctcagagg gatgttgatc agtatattga ggcggcagac
     1501 aaagccgggc tgacgattaa gtatattttt gaaacccatc tgcatgccga ttttgtcagt
     1561 gggcacaaag aattatccga gcgcaccggg gccgatatcg tattcggtgc caaggccggt
     1621 gctgccttcc ctcatatagc tgctcatgac ggcgacatat ttcgagttgg agaggtgtgt
     1681 ctgacgattc tggagactcc cgggcacact cccgagagca tctgcattct ggcgaaagat
     1741 acagcttcca gtaagccgga gaaactattc tcaggcgata ctctttttat tggcgatgtt
     1801 ggtcgtcctg atcttgtcgg cagcaaaggt tatagtgccg aagaaatggc cgccttgatg
     1861 tacgacagcc tgcatgagaa gctgctcaat ctacccgatg atgtagaggt ctatccggcc
     1921 cacggtgccg gctctctttg cggtaaacaa ctctccgatg agcgttcttc cagcatcggg
     1981 cagcagaaga agtttaacta tgctctccag cccatgtcca aggaggcctt tgtggaggtc
     2041 ctcaccgccg atcagcctga agtccctgcc tacttcccta aagatgttga gctgaacaga
     2101 ctgggagctg tgccgttgaa agaacttgcc ctgccccggg ccatgactgc gtctgaaacc
     2161 ttgcagtatc tggagaaagg ggcggtggtg ctggatgtgc gtagtgccgc cgagtacagt
     2221 gcaaatc
//
LOCUS       DAOQNZ010000671         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30770_length_2226_cov_3.186089, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000671 DAOQNZ010000000
VERSION     DAOQNZ010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30770_length_2226_cov_3.186089"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..613)
                     /locus_tag="PKW73_11920"
     CDS             complement(125..613)
                     /locus_tag="PKW73_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74043.1"
                     /translation="MEGDLQNQLIGIFAAQPAPSDTSAADEEHHKQGLRELVGRIWNK
                     LVHWHSKDAAECACRRCDDFISQLHTEGHKLDHAHHAEKLGLTHWNKEELPMYNPPPP
                     YGTGTVDDVVLVRKLMKSEDVLNALANAENQWAKTHSKDCKLGNDYWKTRIEEYKTKE
                     EP"
     gene            789..2195
                     /locus_tag="PKW73_11925"
     CDS             789..2195
                     /locus_tag="PKW73_11925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA74044.1"
                     /translation="MTDFMPEKVEQYDLVVLGGGKGGKSLALALAPMGYKVCLIEAKM
                     LGGSCINVACIPTKTMVAGTRLVYEAGQARARGFKLSLEGNNIQAVIARKRQVVSSMV
                     ETHEKLFTTTENLTFLKGLGSFVASSEGTVIKVELEGSCRYVTGKTIVVNTGTKPLMP
                     IIEGMEQVPLLSSTSIMELDYLPGHLAIIGGGYIALEFAQIFARLGSRVTLLERGTTF
                     LPREDRDIADCVQKILQEEGVEILFDVKVEKLSLDGQDKVIHLNSAGTVREFRATDIL
                     AATGRASVNEALNLAAVGIDVDSRGFIKVNEYLETSRAGVFAVGDCKGGPFFTHASWD
                     DFRILKDNIVHSAGRTTTGRLIPYTLFVEPELGRVGLTEAEALKSGRNIQVLTLPAAK
                     IPRANTEGETRGMLKAIIDRDSKEILGFAALSLYGGEIASAVQVAMLAGMPCNRLGRI
                     VFSHPTMCESLNLLFQGV"
BASE COUNT          494 a          571 c          594 g          567 t
ORIGIN      
        1 gcttcctgcc tgtttctttc gtaagacatg aactggtacc ggaaagaata gatatgtagt
       61 aagttcctgc agcttacagc ctatttgtgt cgaagctgtg atctcttgcc cttactctag
      121 ttgtttaagg ctcttctttg gttttgtatt cttcgatgcg ggtcttccag taatcgttgc
      181 ccaacttgca gtctttactg tgtgtcttgg cccactggtt ctcggcattg gctagagcat
      241 tgagaacgtc ttccgatttc atcagtttgc gcaccagtac cacatcgtct accgtgccgg
      301 taccataggg cggcggtgga ttgtacatag gtagttcttc tttgttccaa tgagtcagac
      361 caagcttttc cgcatggtga gcatggtcta gtttgtgacc ttcagtgtgc agttgggaaa
      421 tgaaatcatc gcaacgcctg caagcgcact ccgcagcatc tttgctgtgc cagtgcacca
      481 atttattcca gatccggcca actagctctc tcaagccttg tttgtgatgc tcctcatcag
      541 ccgcagatgt atcagaaggg gccggttgtg cggcaaagat tccgattagc tggttttgca
      601 gatcgccttc catattaaac ttccttcttt cctcaaatca ctcatatcag catacagtta
      661 catttttaca ccggattcgg atttaactca tcccctgcgg ggatgattga gcttggtatt
      721 aggaccgggc cggttaaact cggacttgct atcatttact accctttagc gtctgtttgg
      781 agacccctgt gactgacttt atgcccgaaa aagtggaaca gtacgacctg gtagtgctcg
      841 gcggcggcaa agggggcaag tctctagctc ttgccctggc tcccatgggc tacaaagtgt
      901 gcctgataga ggcgaaaatg ctgggcggtt cttgtattaa tgtcgcctgt attccaacca
      961 agacaatggt ggccggaacg cgcctggtgt atgaggccgg tcaggccagg gccaggggct
     1021 tcaagctttc tctggaaggc aataatattc aagccgtcat tgcccggaag cgccaggtgg
     1081 taagctccat ggtggaaacc catgagaagc tcttcactac caccgagaat ttaaccttct
     1141 tgaaagggct gggcagcttt gtcgcttcat cggaaggtac cgtcatcaag gtggaactgg
     1201 agggcagctg ccgctacgtc accggtaaga ccatcgttgt caacaccggc accaagccgc
     1261 tcatgcctat cattgagggc atggagcaag tgcctctcct gtccagtaca tcaataatgg
     1321 agctggacta tctgcccggt cacctggcta tcatcggagg cggctatatc gccctggagt
     1381 ttgcccagat ctttgcccgc ttgggtagtc gggttacctt gctggagcga ggcacaacct
     1441 tcctgcctcg ggaggaccga gatattgccg actgtgtgca gaaaatattg caggaagagg
     1501 gagtggaaat cctcttcgat gtgaaggtcg agaagctgag cctggacggt caggataaag
     1561 taattcatct caatagtgcc ggaactgtca gagaatttcg agccactgat atcctggctg
     1621 ctacggggcg cgcctctgtc aacgaggccc ttaacctggc ggcggtgggc atcgatgtgg
     1681 acagccgcgg ttttatcaag gtaaacgaat atctggaaac ctcaagagcc ggtgtcttcg
     1741 ccgtcggtga ttgcaagggc ggccccttct tcacccatgc ttcctgggac gattttcgaa
     1801 tcctgaaaga caacattgtt cacagcgccg gacgcaccac caccggcagg ctcattccct
     1861 acaccctctt tgtggagccg gagttgggaa gggttggact gacggaagcg gaagccttga
     1921 aaagcggtcg gaacatacaa gttttgactc taccggccgc caaaatacca agagccaaca
     1981 ccgaaggcga gactagaggc atgctgaagg ccataatcga tcgggacagc aaagagattc
     2041 taggctttgc ggccctgagc ctttacggcg gcgaaattgc atccgccgtg caagtggcca
     2101 tgcttgccgg tatgccctgc aataggctcg gcagaattgt cttcagtcat cccaccatgt
     2161 gcgagagtct gaacctgctc ttccaagggg tttagagtga aattcgatct ctatctagcc
     2221 cttgga
//
LOCUS       DAOQNZ010000672         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_30848_length_2223_cov_4.079336, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000672 DAOQNZ010000000
VERSION     DAOQNZ010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30848_length_2223_cov_4.079336"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /gene="hutU"
                     /locus_tag="PKW73_11930"
     CDS             complement(<1..1168)
                     /gene="hutU"
                     /locus_tag="PKW73_11930"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNA74045.1"
                     /translation="MSRQASVMHETGAGTDPGTEMIKTILASKEPIEVRAPHGSTLHT
                     KGWVQEAALRMLLNNLDPDVAEKPLELVVYGGSGKAARNWECFKAIVKALTTLESDET
                     LLIQSGKPVGVFKSHENAPRVLIANSNLVGHWATWEHFRELDKKGLAMFGQMTAGSWI
                     YIGTQGILQGTYETFASLARKHYGASLKGKVVLTAGLGGMGGAQPLAITMNEGVALCV
                     EVDDSRIQRRLDTRYLDVRADSLEHALKLADEAKAKGEAISIGIHGNAADILPRMLAL
                     GYMPDALTDQTSAHDPLNGYVPLTEEGEPMTLEAAAKLRQEDPKAYLKLSEAAIARHV
                     EAMLEFQKRGVVTFDYGNNIRQVAFDHGVKDAFNFPGFVPAYIRPLFCEGKGPFR"
     gene            1296..>2223
                     /locus_tag="PKW73_11935"
     CDS             1296..>2223
                     /locus_tag="PKW73_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNA74046.1"
                     /translation="MVKELTKPEAAAKEPKVKKPAKEHETISGVPLKRVYGPEDLKDW
                     KYDEALADPGQFPYTRGIHEDMYRGKVWTMRQFAGFGGPEETNQRFRYLLSQGQTGLS
                     TAFDLPTLMGYDSDHPKAHGEVGVCGVAIDSLEDIEILYKDLPLDKVSTSMTINGPAV
                     IIYAMFLANAKKRGIDWKTLNGTLQNDIFKEYIAQKTYLIPPRPALKLIQDMMVFCTH
                     NVPRWNTISVSGYHIREAGATAVQELAFTLADGFAYVDAGIEAGLQVDDFVPRLSFFF
                     NAHIDFFEEIAKYRAARRIWAKRMRDHYGAKDE"
BASE COUNT          516 a          624 c          597 g          486 t
ORIGIN      
        1 agcggaaggg acctttacct tcgcagaaga gaggtctgat ataggccggc acgaagccgg
       61 ggaagttgaa ggcatctttg acgccgtggt cgaaagccac ctggcgaata ttgttgccgt
      121 agtcaaaggt gaccacgccg cgcttctgga attccagcat ggcttccaca tggcgggcga
      181 tagctgcttc cgaaagcttc aaataggctt tgggatcttc ctggcgcagt ttggctgccg
      241 cttccagggt catgggctcg ccctcttcgg tcaagggcac gtagccgttg agaggatcat
      301 gggcggaagt ctggtcggtg agagcatcag gcatataacc cagggccagc atgcggggca
      361 ggatatcggc ggcattgccg tgaataccga tggaaattgc ctcgcctttt gctttcgcct
      421 catcggccag tttgagagca tgctccagac tgtcggcacg cacatctaag tagcgcgtat
      481 ctaagcggcg ctggatgcgg ctgtcatcca cttccacgca aagggccact ccttcattca
      541 tggtgatggc cagaggctgg gcaccaccca tgccaccaag accggcggtc agcaccactt
      601 tccctttcag gctggcaccg tagtgtttgc gcgccagaga ggcaaaggtt tcgtaggtac
      661 cctgcaaaat gccctgggtg ccgatgtaga tccaggagcc ggcagtcatc tgaccaaaca
      721 tggccagacc tttcttatcg agttcgcgga aatgttccca ggtggcccag tgacctacca
      781 gattggaatt agcgatgaga acccgggggg cattctcatg actcttgaat acacctaccg
      841 gttttcctga ctggataagt aaggtttcat ccgactccag ggttgtaaga gctttgacaa
      901 tggctttgaa acactcccag ttacgcgcag ccttgccgga gccgccgtag accacaagtt
      961 ccagcggttt ttccgctaca tccgggtcaa ggttgttgag caacatgcgc aatgccgctt
     1021 cttgcaccca acccttggta tgcagggtgg aaccgtgcgg ggcgcgcact tcaatgggct
     1081 ccttgctagc caaaatagtt ttaatcattt cagtacctgg atcagtaccc gctccggttt
     1141 catgcatgac acttgcttgg cgcgacatag ggaaaatgcc tctttgctga gaaagtatgt
     1201 aaaaattaca gagtgacgtg gttctatccc acggcaatgt ctccaaatga tatccaattt
     1261 ttggactcag gagcaagaat accaaggaga tgctcatggt caaggaactg actaaacccg
     1321 aagcagcggc caaagagccg aaggtcaaga agcccgcgaa ggagcatgag accatttcag
     1381 gcgtgccgct caagcgagtt tatggtccgg aagacctgaa agactggaag tatgacgagg
     1441 ccctggccga tccaggtcag ttcccttata ccaggggcat tcacgaggac atgtaccgcg
     1501 gcaaggtctg gaccatgcgg caattcgccg gtttcggcgg accggaggaa accaaccagc
     1561 gcttcagata ccttttgtct caaggacaga ccggtctctc cacggctttc gatctcccca
     1621 ccctcatggg ttacgattcc gaccatccca aagcccacgg cgaagtcggc gtctgcggag
     1681 tagccatcga ctccctggaa gacattgaga tcctctataa agatctcccc ctcgacaaag
     1741 tttccacttc catgaccatc aatggtccgg ccgtgatcat ttacgccatg ttcctggcca
     1801 acgccaaaaa gcgcggcatt gattggaaga ccctgaacgg cacgctccag aatgacattt
     1861 tcaaagagta catcgcccag aagacctatt tgattccgcc ccgcccggcc ctcaagctca
     1921 tacaagacat gatggtcttc tgcacccaca atgtgccgcg ctggaatacg atttcggtga
     1981 gcggctatca cattcgggaa gccggtgcca cggccgtgca ggagttggct ttcaccctgg
     2041 ctgacggctt cgcttatgta gatgccggaa tcgaggccgg tcttcaagta gacgacttcg
     2101 tgccgagact gtccttcttc ttcaatgccc atatcgactt ctttgaagaa atcgccaaat
     2161 accgtgccgc tcgacgcatc tgggccaaac gcatgcgtga ccactacggc gccaaagacg
     2221 aac
//
LOCUS       DAOQNZ010000673         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31024_length_2217_cov_3.595745, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000673 DAOQNZ010000000
VERSION     DAOQNZ010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31024_length_2217_cov_3.595745"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PKW73_11940"
     CDS             <1..1285
                     /locus_tag="PKW73_11940"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HNA74047.1"
                     /translation="RGALQVVPHQGWLADVEDLLACEKDGKFRMDAFYRRLRQKTNIL
                     MEAGKPQGGKYSFDSENRLPWKGEPQPPAVPLFSPDEITKEVGALIESRFARHPGQLS
                     LEHLPASQADADHLWQWAKENCLPHFGPYEDAMSSKSSGLFHTRISPLLNIHRILPGQ
                     VVEDVMNMDLPLASKEGFIRQVLGWREFVRAVHVHTDGFRKGRARAEIRSSPGDAGYG
                     AWSGHSFDGSQQENHFSGDGGAAPNYLESHEDLPPAFWGQPCGLNCLDHVVQDVLKEG
                     YSHHITRLMILCNLGTLLDIEPRQLSDWFWVAYADAYDWVVEPNVLAMGTFSLADMIT
                     TKPYVSGAAYINKMSDYCRSCRFDPKSCPVTRLYWAFLERKWEKLKDNQRLSMPINSL
                     KKRSPQDKEKDRAVFQKLTALLKAGQVAIPEQFTQ"
     gene            1385..2029
                     /locus_tag="PKW73_11945"
     CDS             1385..2029
                     /locus_tag="PKW73_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74048.1"
                     /translation="MKPVNNNALPQTSAQGLSQHILENYRQLSSSVRQDYLDYLTAKT
                     RDYAKSVSVRCESGTSQGNEMKALLERIYFTLEPYAVELNRVNGSDARILELSVQPPC
                     LTNELADGSTQRRADRTVSYYRCRFSTRLLALVIRGSEDCIDFFLIPTDRVLGLSLIE
                     AQTKPLMSFTFEHAQGWTVEGKELNHDRLERYSLLTLEHLLDRTQEEQSLYQRR"
BASE COUNT          575 a          564 c          575 g          503 t
ORIGIN      
        1 ccgtggtgca ttgcaggtgg taccacacca aggctggctg gcagacgtgg aagacttgct
       61 tgcctgtgaa aaggacggca agtttcgcat ggacgccttc tacaggcgcc tcaggcagaa
      121 aacgaatatt cttatggaag caggcaaacc acagggaggg aaatacagct tcgatagcga
      181 gaatcggctg ccctggaaag gtgagccgca accaccggcg gtgcctttat tttctccaga
      241 cgaaatcact aaggaagtgg gcgctcttat cgagagccgg ttcgcaaggc atcctggcca
      301 actatcgcta gagcatctgc ccgccagcca ggctgatgcc gaccatctct ggcagtgggc
      361 aaaggagaat tgcctgccgc atttcggtcc atacgaagat gctatgtcca gtaagtccag
      421 tggacttttc cataccagaa tctcgccact tttaaatatt catcgaattc ttcctggtca
      481 ggtggttgaa gacgtgatga atatggatct accgcttgcc agtaaagaag gttttatcag
      541 acaggtgctg ggttggcggg agtttgtcag agcggtgcat gtacacacag acggcttccg
      601 caaagggcga gcccgggcag agatccggag cagccctggt gatgcaggct atggagcctg
      661 gtctggacac agttttgacg gctcgcagca agagaatcac ttctctggcg atggcggtgc
      721 cgcccctaat tatctcgaaa gccatgaaga cctacccccg gctttctggg gacagccctg
      781 cggtctcaac tgcctcgacc atgtggtgca agacgtcctt aaagaaggct acagtcatca
      841 cattacgaga ctgatgattc tttgcaatct cggcacctta ctggatattg agcctagaca
      901 attgagcgac tggttctggg tggcttatgc cgatgcctac gactgggtag tagaaccaaa
      961 tgtgctggcc atgggcacct tcagtctggc cgatatgatc accaccaagc cctatgtgag
     1021 cggggcggcc tacatcaata aaatgagcga ctattgccgc agttgccgtt tcgatcccaa
     1081 aagctgccct gtaaccaggc tctactgggc atttttggag aggaagtggg agaaactcaa
     1141 ggacaatcaa aggctgtcca tgcccataaa ttctctaaaa aagcgctcac cacaggataa
     1201 ggaaaaggac agagccgttt tccagaaatt aacagccctc ttgaaagctg gacaggttgc
     1261 cattccggag caatttaccc agtaatatat acctttgcct ctgataatta gttaactaca
     1321 gcaagttgag gaagatagct attcttagac aggttgtcat gcccccagag ataagtacag
     1381 ttcaatgaag ccggtaaaca ataatgcttt gcctcaaact tcagcgcagg ggctgagcca
     1441 acatattctg gaaaactaca gacagctatc atccagcgtc cggcaggact atctggatta
     1501 tctaaccgct aaaaccaggg actacgctaa atccgtcagc gtcaggtgtg aatcaggcac
     1561 cagccagggt aatgagatga aggctctttt ggagagaatc tatttcactc tggaacctta
     1621 cgcggtggag ttgaatcgag tcaatggcag cgatgccagg atactggagt tgagcgtaca
     1681 accgccctgt ctcactaatg aactggccga cggcagcacc caacggcggg cagatcgtac
     1741 cgtcagctat taccgctgtc gcttttccac acggcttttg gccctggtca tacgaggcag
     1801 tgaagactgc atcgattttt ttcttatccc caccgaccgc gtgcttgggc tttctctcat
     1861 agaagcccag accaagccct tgatgagctt cacttttgaa catgctcagg gctggaccgt
     1921 agaaggcaag gaacttaatc acgatcgcct ggagcgctac agcttgctca cactggagca
     1981 tttactggat cgcacccagg aagaacagag cttgtaccag cgccgctgaa ccgtcacttc
     2041 tggtcagcgg tcaatttgag tctggcaatc gaccttaaag gggctaaaat aatgtcctcg
     2101 ttagggttca ggatcagttt atggctaaag acaccagcct tgtatcactg ctagacaggc
     2161 acattcgaac cttaagcctg tccataggtg aaagacattg caaaaagcct gatgcac
//
LOCUS       DAOQNZ010000674         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31030_length_2217_cov_3.130897, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000674 DAOQNZ010000000
VERSION     DAOQNZ010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31030_length_2217_cov_3.130897"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKW73_11950"
     CDS             complement(<1..288)
                     /locus_tag="PKW73_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74049.1"
                     /translation="MLDASERKKLGGLIVELGLLDNRDLGQALSIAQETSLPLGRVLV
                     LSEMLTEETLQGVLRCQSLMKQNLVDLDLARKAMKLVAQDGVSIDDALFKLG"
     gene            complement(445..1008)
                     /locus_tag="PKW73_11955"
     CDS             complement(445..1008)
                     /locus_tag="PKW73_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA74050.1"
                     /translation="MSIFSPLNPLEPLALETERVVLRRWRKSDFKPFARMNQDPMVME
                     FFPRRLDADETRAMIRRIESGFEEHGFGLYAVELKENNGFIGFVGLSVPKFSTHFTPC
                     VEIGWRLSSEYWGRGLATEAAREVMRAAFHDFGQEEVVSMTAIPNQRSRRVMEKLGMT
                     TLPDDDFDHPLVPEGPLKRHVLYRKRR"
     gene            1143..1781
                     /locus_tag="PKW73_11960"
     CDS             1143..1781
                     /locus_tag="PKW73_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74051.1"
                     /translation="MANTNTPNLQEPDLTKEDWRVIWGFLALFGLVAPIAMPVTAILA
                     QVMVWQYVLAGMPQVTFSFGFLESCLLVICISLSAILINIGITQLVLLKSGKTFKEYS
                     LAERRNREAKMAELKEQKKLFDSSTVSKYLVYFARQFALLSICLVAASHYLPGKLGFE
                     SIGALLNCAFVLTGIREMLCLFWSMILSFGVDRLKERFPQAAQTAQEGNNDL"
     gene            1841..2092
                     /locus_tag="PKW73_11965"
     CDS             1841..2092
                     /locus_tag="PKW73_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74052.1"
                     /translation="MNLRKKLIDYITGVAVVGAISLVSFVLHVLFPKNGHGSTVSIQL
                     FSPSFWVAWTTLMLVFALFYALWVFFLYNYNLAGRSRSR"
BASE COUNT          559 a          629 c          530 g          499 t
ORIGIN      
        1 gcccagcttg aagagagcat catcaatgga aacgccgtct tgagctacca gcttcatggc
       61 tttgcgagcc agatcaaggt ccaccagatt ttgcttcatc aagctctgac aacgcaaaac
      121 cccttgcagg gtttcttccg tcagcatttc cgagagtacc agcactcgcc ccaggggcaa
      181 actggtctcc tgggcgatag agagcgcctg tcccagatcg cgattatcca acagtcccag
      241 ttcaacaatc agtccaccga gtttcttcct ctcgctggca tccaacatag gcaaatctcc
      301 ctaagccacc acaagttccc gggggtccaa aggaaccccg cctaaagcaa aatgtacccg
      361 cctgccggca aaacttatgc aaaagcaaag aaggatatac ctacgaatcc ttccaagccg
      421 ataaagcgtc ttctgtccct gagatcagcg gcgcttgcga taaagcacat ggcgtttgag
      481 cggaccctcc ggcaccagcg ggtgatcgaa atcatcgtcc ggcagagtgg tcatgcccag
      541 tttttccatt acccgccttg agcgctgatt gggaatggcg gtcatactga ccacttcttc
      601 ctgaccgaag tcgtgaaagg ccgctctcat cacttcccgg gccgcctcag tagccagacc
      661 gcgcccccag tactccgagg aaagacgcca gcctatttcc acacagggtg tgaagtgagt
      721 gctgaattta ggtacggaaa gtcctacaaa acctatgaac ccattgttct ctttcagttc
      781 aacggcataa agcccgaaac cgtgctcttc aaaaccgctt tcaatgcggc gaatcatagc
      841 ccgtgtttcg tccgcatcga ggcgacgggg aaagaattcc atgaccatgg ggtcttgatt
      901 catgcgggcg aagggtttga aatcactttt gcgccagcgc cgcaagacaa ccctttcagt
      961 ctcaagggcg agcggttcca ggggattcaa tggactgaag atactcatgg ctcactttcc
     1021 ccaaatgttg aacaattcat tagatgcgta acctttcatg ttgtcaagtg taagaatgga
     1081 gctatcaaag aaaaacgtat tagagccgaa actctcaaca gaataaaagc aagagcaccg
     1141 aaatggcaaa cactaacacc cccaacctgc aggaacctga tctgacaaaa gaagactggc
     1201 gggtgatatg gggcttcctg gccctttttg gactggtggc gcccatagcc atgccggtca
     1261 cggccatact tgcgcaagta atggtctggc aatatgttct ggccggcatg ccgcaggtca
     1321 ccttttcttt tggttttctg gaaagctgcc tgcttgtaat ctgcatcagt ctatccgcca
     1381 tcctcatcaa cataggtatc actcaacttg tcctgttgaa gtcaggcaaa accttcaagg
     1441 agtactccct ggccgagcgc cgtaaccgcg aagccaaaat ggcggagctt aaagaacaga
     1501 agaagctgtt cgatagttct accgttagca agtacctggt ctacttcgcc agacagttcg
     1561 ccctgctttc catctgcctg gtagcagctt cacattattt accgggtaag cttggcttcg
     1621 aatcaatcgg agccctgcta aactgcgcct tcgtactcac aggcataaga gaaatgctgt
     1681 gcctcttctg gtccatgata ctctccttcg gcgtcgatcg cctgaaagaa cgatttcccc
     1741 aggcagcgca gaccgcacag gaaggcaata acgacctttg agggtacaac aatctggtag
     1801 cagccaattc caccggaaac agcaagggag atatcgccaa gtgaaccttc gcaagaagct
     1861 tatcgactat ataaccggcg tggcggtggt gggtgccata tctttggtca gcttcgtgct
     1921 gcatgtgttg tttcccaaaa acggacacgg aagcacggta tccattcaac tgttcagccc
     1981 cagcttctgg gtggcctgga cgactctcat gctcgtcttt gccttgttct atgccctctg
     2041 ggtcttcttt ctctacaact acaatcttgc cggtcgcagc aggtctagat agctataacc
     2101 gcagaaaatc cggacggaaa gacagacggc agcgccgcag gcgccggccg actgagagcc
     2161 gaccgacgcc tgcaaaacac ctgagctaaa ggctttagaa atcgccttct cgcacct
//
LOCUS       DAOQNZ010000675         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31124_length_2214_cov_3.096804, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000675 DAOQNZ010000000
VERSION     DAOQNZ010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31124_length_2214_cov_3.096804"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKW73_11970"
     CDS             complement(<1..697)
                     /locus_tag="PKW73_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HNA74053.1"
                     /translation="MSKGRNDLAIWLVRKEGEPLALELAEALQAQVFKPWLSDKKAIE
                     QFREHFASFSKWLFIGTTGIASRYLQGLLQDKKQDPCLVVLDEGCGFAVSLAGGHEGG
                     GNALAYKVAALVQAVPVVTTASESIKPLVVGIGCRKGVAADTIEAAVNKALAGRSLQE
                     VREFATIDIKAQEPGLLELSARHDIPLRIFKQEDIAARQWSTRPASHVLETVGVDGVC
                     EPCALMASARGSLL"
     gene            complement(694..1467)
                     /gene="cobM"
                     /locus_tag="PKW73_11975"
     CDS             complement(694..1467)
                     /gene="cobM"
                     /locus_tag="PKW73_11975"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HNA74054.1"
                     /translation="MKVYFIGAGPGAGDLITIRGARALGKVSLVLYAGSLVPPALLEH
                     CRPDADIINTAGLNLEEQIGHYLHAQANDFDVARLHSGDPAIYGATGEQIQELKKHGI
                     DYEIIPGVSSFTAAAAELGLELTRPTVSQTIILTRVSGRASAVPELESIDKLAYHQAT
                     LCIFLSGPHLRKIVDDLMQHYAESTPVALVYRATWEDQRTHRSTLGRVLEEVAVDDWK
                     LTTMLLVGAVLDDEIEAASKLYAADYSHKFRKARVPVQS"
     gene            complement(1464..>2214)
                     /gene="cobI"
                     /locus_tag="PKW73_11980"
     CDS             complement(1464..>2214)
                     /gene="cobI"
                     /locus_tag="PKW73_11980"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HNA74055.1"
                     /translation="ITVQAAEVLQNCQVILCPKANRAKNSVAKDSIAALNLPAERFQI
                     VEYDMEEDRQEAFKTYKKLAAEIVNTIKEGKNVAYLTIGDALTYSTYGYTLTALKALD
                     PNLSITTIPGITSFSLLAALSGYPLGQGKERLLILPCPEQASDLKQDIESHDLVILMK
                     IGRRLPMVLELLHEMDIARHCVLGSRLGLPTQFIETAENLEAGEKGYLSTMLIRKTAP
                     VSPWLHLNQEAATCRTETASNEDPQLEAIKR"
BASE COUNT          424 a          562 c          654 g          574 t
ORIGIN      
        1 ccagtaaact gccgcgtgcg ctggccatga gggcgcaggg ctcgcagacg ccgtccacac
       61 ccacggtttc taggacatgg ctggctggtc ttgtgctcca ctgtcttgct gcaatatctt
      121 cctgtttgaa gattctcaaa ggaatgtcat gacgggcgct aagctcaagt agtcccggtt
      181 cttgcgcttt aatgtcgatg gtggcgaatt ctcttacttc ttgcaggctt cgaccggcca
      241 gggccttatt gacggcggct tcgatggtgt cggcggcaac gcctttgcgg cagccgatgc
      301 ccaccaccag cggtttgatg ctttcgctgg cggtagtgac tacggggacg gcctgcacga
      361 gagctgctac tttgtaggcg agggcattgc cgccgccttc gtgaccgcct gccaggctga
      421 cggcaaagcc gcaaccttcg tcgagcacta ccaggcaggg gtcctgtttt ttgtcttgca
      481 gaagtccctg caggtagcgg ctggcgatgc cggtggtgcc gatgaagagc catttcgaaa
      541 aggacgcaaa gtgttctcta aattgctcga tggccttttt gtcactcagc cagggcttga
      601 agacctgcgc ttgcagcgcc tctgccagtt caagggcgag gggctcacct tcctttctta
      661 ccagccagat ggccaggtcg tttcttccct tgctcatgac tgaacaggca cccgggcctt
      721 tctgaatttg tgactgtaat cggcggcata caattttgat gcggcttcta tttcatcatc
      781 gaggacggct ccgacaagca gcatggtggt cagtttccag tcgtccacag ctacctcctc
      841 cagtactcta ccaagggtac tcctgtgagt gcgctggtct tcccaggtgg ctctgtagac
      901 gagagctacc ggcgtggatt cggcataatg ctgcatcaaa tcgtcgacga ttttccttaa
      961 atgcggtcct gacagaaaga tgcagagcgt ggcctggtga taagccagtt tgtcgatgga
     1021 ctcaagctcc ggtacggctg aagccctgcc gcttaccctg gtgaggatga tggtctggga
     1081 gactgtcggt cgtgtcagtt caagaccaag ttcggcagcg gcagctgtga aagaggagac
     1141 gccgggaatg atttcgtagt cgatgccgtg tttttttagt tcttgtatct gttcgccggt
     1201 ggcgccataa atggccggat cacctgagtg caggcgagct acatcgaaat cattggcctg
     1261 ggcatgcagg tagtggccga tttgctcttc taggttgaga ccggctgtat tgattatgtc
     1321 ggcatcgggg cgacagtgtt caagcaaggc tggtggcacg agagaaccgg cataaagtac
     1381 caggctgact tttcccaggg cccgggctcc ccgaattgta atcagatcgc cggcaccggg
     1441 cccggcgcct ataaagtaca ctttcatctt tttatggcct ctagttgtgg atcttcgttg
     1501 cttgctgtct ctgttcggca ggttgccgct tcctgattga gatgaagcca ggggcttacg
     1561 ggggctgttt ttcgaatcag catggtggaa aggtaaccct tttctcccgc ttccaggttc
     1621 tcagccgttt ctataaactg tgtgggcaag ccgaggcggc tacccaggac acagtgtctg
     1681 gcgatgtcca tctcatgtaa taattccagc accatgggca ggcgtcgacc gattttcatc
     1741 aagataacaa ggtcgtgact ttcaatatct tgtttcaagt ctgaggcttg ctccgggcaa
     1801 ggcagaataa gcagtcgttc tttaccctga ccaagcggat aaccgctgag ggcggccagc
     1861 agactgaaag aggtgatacc ggggatggtg gtgatggaga ggttcgggtc cagcgctttc
     1921 agggctgtga gcgtgtagcc gtatgtggaa taggtaagtg cgtcgccgat ggtcaggtaa
     1981 gctacgttct tgccttcttt gatggtgttg acgatttctg cggccagttt cttgtaggtc
     2041 ttgaaggctt cctggcggtc ttcttccatg tcgtattcca cgatctgaaa gcgttccgca
     2101 ggcaaattga gagcggcgat ggagtctttt gctacgctgt ttttagcgcg gttggctttg
     2161 gggcaaagaa tcacctgaca gttttgcaag acttcggccg cctgtacggt gata
//
LOCUS       DAOQNZ010000676         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31162_length_2212_cov_4.175243, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000676 DAOQNZ010000000
VERSION     DAOQNZ010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31162_length_2212_cov_4.175243"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKW73_11985"
     CDS             <1..362
                     /locus_tag="PKW73_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74056.1"
                     /translation="KVEKHDSDAAPKQPAEAKGDNETYDMTYGSPAYWSLMRRSGNST
                     ENDQKDYESQKPGETDKSRETYEMTEGSPAHRALMRRMAQPDENRQSRQAEDKTLNTY
                     RDRFGGSALPGLVIEGI"
     gene            complement(447..1532)
                     /locus_tag="PKW73_11990"
     CDS             complement(447..1532)
                     /locus_tag="PKW73_11990"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74057.1"
                     /translation="MSATDAAELRKSLEIAEKEHGQKSELLIEPLEKLAYAYHRAGAY
                     DDAEACFKRVLLLKETFQPANWQSMLDIYHGLAILLRVQNRFADAEPYYLKAIELSER
                     LVGASHPDTFTRKNYLAGLYFAWGKYEMARNLVEASYKVYQATFGENSEVVGIAAMGL
                     ALICNRQGRGPDAANYFKQATRLLPAGPGGAVILDFQDLAASLFFLAKEKYKQGMIEQ
                     AEVLFRYSLLIETDRLWPGHPIVGDNVQLLGDLYRSQAMPVQAEFLYRKALEIRRAVM
                     GPDHLDVAISANCLGVLLCDNHRYKEAEPFLLEACEIRKRAGFPPVYANSLRAYAGCL
                     RHLDRLDEANECEQKARSIYDRYNQSS"
     gene            complement(1552..>2212)
                     /locus_tag="PKW73_11995"
     CDS             complement(1552..>2212)
                     /locus_tag="PKW73_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA74058.1"
                     /translation="VQLASLVLRWLTKEGHVVEHVASGGEGLQRLRFDPYDLIVLDWN
                     LPEVEGIDILKQFRRSGGTTPILMLTGKKAIEEKLEGLSEGADDYLTKPFDGRELTAR
                     ITALLRRPAVLLDTCLKVGDIELDTKAHSVKRAGKPLTLLPKEFALLEFLMRRPGEFF
                     QAQRLLEHVWPSESDSTTEALTSCIKRLRQKLDNPDAESVIRNVRGAGYGLFVDMETN
                     C"
BASE COUNT          499 a          631 c          587 g          495 t
ORIGIN      
        1 gcaaggttga gaagcacgac agcgacgccg ctcccaagca acctgctgaa gccaagggcg
       61 ataacgaaac ctatgatatg acctatggct ctcctgccta ctggtccctc atgcgccgtt
      121 ccggcaactc cactgaaaat gatcagaaag attatgaaag ccagaagcca ggcgaaaccg
      181 acaaatcacg cgaaacctac gagatgaccg aaggttcccc ggctcaccgc gccctcatgc
      241 gcagaatggc ccagcctgat gagaacaggc aatcgcgcca ggctgaagac aaaaccttga
      301 acacctaccg cgatcgcttt ggtggttctg ccctgcccgg tctggttatc gaaggcatct
      361 aacgtgactg agcgcatctg actcgactga tcgaaggtat ctagctcgac tgatccaagg
      421 catctgattc gcttgctcga aggcatctag ctcgactgat tgtacctgtc gtagatgctg
      481 cgcgcctttt gctcacattc gttagcttca tccaacctgt ccaggtgacg aaggcaaccg
      541 gcataagccc gcagagaatt ggcataaaca ggcggaaagc ccgccctttt gcgaatctcg
      601 caggcttcca gcaagaaggg ctcggcttct ttgtagcggt gattgtcgca aagtagcacc
      661 cccaggcagt tggcactgat ggctacatcg agatggtccg gtcccataac ggcgcgccgt
      721 atttccaggg cttttcgata aagaaactcc gcctgcactg gcatggcttg cgagcgatag
      781 agatccccca gaagttgtac gttgtcgccc acgatcggat gccccggcca gaggcgatcg
      841 gtctcaatca aaagtgagta gcgaaacaat acttccgctt gttcgatcat gccctgcttg
      901 tacttctctt tggctaagaa aaagagactg gcagccagat cttgaaagtc gagaatgacg
      961 gcgccaccag gcccggccgg caaaaggcgg gtggcttgtt tgaaataatt tgccgcatcg
     1021 gggcctcgcc cctgcctgtt gcagatgaga gccagtccca tagccgctat gcctaccact
     1081 tcgctattct cgccgaaggt ggcttgatag accttgtagg aagcctccac aaggttgcga
     1141 gccatttcat acttgcccca ggcaaaatag agaccggcta gatagttctt tcgggtgaac
     1201 gtgtcggggt gcgacgctcc caccaggcgc tcgcttaact caattgcttt caggtagtag
     1261 ggctcggcat cggcaaaacg attttgcacg cgtagtaaaa ttgccagccc gtggtaaata
     1321 tcgagcatgc tttgccagtt cgccggctga aaggtttctt tcagcagcaa gacccgtttg
     1381 aagcaggctt ccgcatcgtc ataggcgccc gctctgtggt aggcataggc gagcttctcc
     1441 aggggctcta tcaataattc gcttttctgg ccatgctctt tctcggcaat ctctagagac
     1501 ttgcgaagct cggctgcatc ggtagcgctc atgtggtgat tacctttcga actagcaatt
     1561 ggtttccatg tctacaaaga gcccgtagcc tgcgccgcgc acgtttcgta tcaccgactc
     1621 ggcatcgggg ttgtcgagct tttgtctgag ccgcttgatg cagctggtga gcgcctcggt
     1681 ggtagagtcg ctttcactgg gccagacatg ttcgagtaag cgctgggcct gaaagaactc
     1741 gcccggacgg cgcatgagaa attccaataa agcaaactct ttcggcagaa gcgtcagcgg
     1801 ctttccggca cgttttacag agtgggcttt ggtatcaagt tcgatatcac caaccttgag
     1861 gcaggtgtcg agtaaaacag ccggtcggcg aagcagtgcc gtgatgcgag ccgtcaattc
     1921 cctgccgtca aaaggcttgg ttaagtagtc gtcggcccct tccgaaagcc cctcaagctt
     1981 ctcttcaata gccttcttgc cggtcagcat caaaatcggc gtggtgccac cgcttcggcg
     2041 aaactgcttg agaatgtcaa tgccttccac ctccggcaaa ttccagtcga gcacaatcaa
     2101 gtcgtagggg tcgaagcgca gcctttgcag cccctcgccg ccgctcgcca catgctctac
     2161 cacatgccct tctttggtca gccatctgag cacgagtgag gctaactgca cg
//
LOCUS       DAOQNZ010000677         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31219_length_2210_cov_3.575406, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000677 DAOQNZ010000000
VERSION     DAOQNZ010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31219_length_2210_cov_3.575406"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKW73_12000"
     CDS             complement(<1..257)
                     /locus_tag="PKW73_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNA74059.1"
                     /translation="MSAQSKTITVTIRYFALLREETGKACETLTLEQGTLADLYKHLQ
                     ERYKLSLPIEAIRAAVNNRFCSWQTDLGDGAEIVFVPPVAGG"
     gene            351..>2210
                     /locus_tag="PKW73_12005"
     CDS             351..>2210
                     /locus_tag="PKW73_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNA74060.1"
                     /translation="MMESQADRGGASLTEPARAAYGAWLSPLSAADVARAAIRLSQTM
                     YGPDGKTLYWLEGRPQEGGRQVLVRLDEHGQVREINPAPLNVRTRAHEYGGGAFTVGP
                     YQGRETVFYCNFADQRLYRQVPGENSEPVAVTSAGRMRYSDLVLDQNRDRLLLVVEDH
                     TGIDEGLLPEIGEIAVEPINYLASLDPGAGGDAEAMMLTCGYDFFAFPRLSHDGKRLA
                     YMAWKHPNMPWDESVLFTAEVGADGSLANHRKVAGGADVSIFQPQWGRDGTLYFVSDE
                     SGFWQLKAIRDFFTMDVIDVSTEKFKNCEFGLPMWVFGQSTYAVVDDEIIAAVNLRGL
                     WKLLRIKVRFEGEDGLGYVCHQEVEVLSSPYSEFSYLTAGSGRVAMLAGAAGIANTVV
                     EYDLATGQFQELCSSAPASLDPGYLSSPEVIEFPTSGGRTAYAFYYPPTNKDFQAQES
                     GQLPPLLVKCHGGPTGAASSLLSPALQYYTSRGFAVVDVNYGGSTGFGRAYRKRLNGN
                     WGVVDVEDCTNAVKYLVEQGKVDGTRVAITGGSAGGFTVLCALTFTDVFKAGASHYGI
                     GDLEALVRDTHKFESRYLDNLVGPYPFSKQVYQARSPIHHTEKLSCPVIFFQGL"
BASE COUNT          423 a          581 c          658 g          548 t
ORIGIN      
        1 cctcctgcta cgggcggtac aaagactatt tcggcgccat cgcccaggtc ggtctgccaa
       61 ctgcagaagc ggttgttcac cgcggcccgt atggcttcta tgggcaggga aagtttgtaa
      121 cgctcttgta agtgcttgta aaggtctgcc agcgtgcctt gctcgagggt gagagtctcg
      181 caggctttgc cggtttcttc ccgcagaagg gcgaagtagc ggattgtcac cgtgatggtt
      241 ttgctttgtg ccgacatcgg atttaactct caggcttcct gaaatttgcc gtttaatcta
      301 gcactatagc gcaaattata tactgtggat ttacttaagg tggggacgtc atgatggaga
      361 gccaggcaga tcgaggtggt gcatccttaa ctgagccggc cagggctgct tacggcgcct
      421 ggctgtcgcc tctgtcggcg gccgatgtgg cccgtgctgc cattcgcctc agtcagacca
      481 tgtacggacc ggacggtaag actctgtact ggctggaagg gcgcccccag gaaggcggtc
      541 ggcaggtgct ggttcggctt gatgagcacg gtcaagtaag ggagatcaac ccggctcctt
      601 tgaatgtgcg tactcgggcc catgagtatg gcggtggtgc cttcacagta ggcccctatc
      661 aggggcggga gacggttttc tattgcaatt ttgccgacca gcgtctctat cgtcaggtgc
      721 cgggcgaaaa ttctgaaccg gtggcagtca ccagtgccgg ccgcatgcgc tacagcgatc
      781 tggtgcttga tcaaaatcga gatcgcctgc ttttagttgt ggaagatcac accggcatcg
      841 acgaaggtct gctgcctgaa attggtgaga tagccgtgga gccgattaac tatctggctt
      901 cgctggatcc cggtgccggc ggtgatgccg aagccatgat gctcacttgc ggttatgact
      961 tctttgcctt tccacgtctc agtcatgatg gtaagcgact tgcctatatg gcctggaagc
     1021 atcccaacat gccctgggac gagagtgttt tgtttacggc cgaagtagga gcggacggct
     1081 ctctggccaa tcatcgcaag gttgcaggcg gtgccgatgt ttccatcttt cagccccagt
     1141 ggggccggga cggcaccctc tacttcgtca gtgacgaaag tggtttctgg cagctcaagg
     1201 ctattcgcga cttcttcacc atggatgtta tagatgtttc cacggagaaa tttaaaaact
     1261 gcgagtttgg tctgcccatg tgggtttttg gtcagtcgac ctatgccgtg gtagatgacg
     1321 agatcattgc cgccgtcaac ttgcgaggtc tctggaagct gcttcgcatc aaggtgcgct
     1381 ttgaaggaga ggatggtctg ggttatgttt gccaccagga agtggaagtt ctctccagcc
     1441 cctacagcga attttcctat ctgacggccg gcagcgggcg agtggccatg ctagccggag
     1501 ctgccggtat tgccaatacc gtggtggagt acgatcttgc caccggtcaa ttccaggaat
     1561 tgtgcagctc ggcaccggcc tctctagacc caggttatct ttcctccccc gaggtaatcg
     1621 aatttcctac ctccggcggg cgcacggctt atgccttcta ttatccgccc accaacaaag
     1681 attttcaggc tcaagagagc gggcagttac cgcccctgct tgtcaaatgt catggcggtc
     1741 cgacgggggc ggcttccagc ctgctctcgc cggcattgca gtattacact tcccggggct
     1801 ttgccgtggt ggatgtaaat tacgggggca gcaccgggtt tggccgggct taccgcaagc
     1861 gccttaacgg taactggggc gtagtagacg tggaagactg tacgaatgcc gtcaagtatt
     1921 tagtggaaca gggcaaggtg gatggtacgc gggttgccat taccggtggt agcgccggcg
     1981 gttttaccgt gctttgcgcc ctcactttta ccgatgtctt caaagccggc gccagccatt
     2041 acggaatcgg cgacctggaa gccctggtca gggatacgca caagttcgaa tctcgctatt
     2101 tggataatct ggttggtccc tatcctttca gcaagcaggt ctaccaggcg cgctctccca
     2161 ttcatcacac ggaaaagctg tcctgtccgg tgatcttttt tcaggggctg
//
LOCUS       DAOQNZ010000678         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31396_length_2203_cov_3.350559, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000678 DAOQNZ010000000
VERSION     DAOQNZ010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31396_length_2203_cov_3.350559"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKW73_12010"
     CDS             <1..760
                     /locus_tag="PKW73_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74061.1"
                     /translation="DPRSIKKYISLHGLMRLATEIGATGYKQQLANALRQNLLERGYI
                     FQEAERQRAGQMLCLLAVLAESFAAILVFTTIKNPIDAALIFLSALLGAGFIKVTLGA
                     REFIPYYSELMAVLEHTHNANLRVKALQFFLKVVNGLLFTVSYAAFALVLAFGSFLLY
                     VVHAVGDLGTYLALLAQLLTQLAILTCFIEAHLLYFHDSPTPEGQAALQALKETYKGK
                     STIDTLKSMLTGTTYESDMSYMLAIYGPESLLFL"
     gene            819..1655
                     /locus_tag="PKW73_12015"
     CDS             819..1655
                     /locus_tag="PKW73_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA74062.1"
                     /translation="MNLPQGMTAIITGASTGIGRGLALSLTKIYKAKLVLTARSREDL
                     ETLAQEVKRLGGEAVVLAADIAGEGVTESLVNLAVKNFGKLNMVVNNAGMGISGSFAK
                     LSEEDWRRIFEVNFFSALTLTQHALPHLRAEEVAKLVNISSVAGKVSIPGSVSYCSTK
                     FALTALSEGLAAELAPDNIDVLTVCPGWVRTEFFQKNDLPNTRNPTEIARRNDLKGFL
                     MKNVLSISTEECVKEIIEALEKGGSRELILTLPGKVAERLQGTAPGVMRFLARQIRPE
                     YK"
     gene            complement(1667..>2203)
                     /locus_tag="PKW73_12020"
     CDS             complement(1667..>2203)
                     /locus_tag="PKW73_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74063.1"
                     /translation="LHAFKAQTAYLACFLNRFAVSLKVAGHISSNLSVYIDGADRALD
                     AGTIEFSADACGCPGLFYLASMQSLSSMHRPMHMHADLPERLLDRANLLISFAVGQRD
                     ANQLGRRIFPSLGLRIEAPTARSLFAGASEKPRFFSDQDYDKFYQDLLTNLLPGTFFC
                     RFTTPVESVVKVQLPEQP"
BASE COUNT          564 a          596 c          556 g          487 t
ORIGIN      
        1 agaccctcgc agcatcaaaa aatatatttc tttgcacgga cttatgcgcc tggctacgga
       61 gattggcgcc actggctata agcagcaact agccaacgct ctcagacaaa atctgcttga
      121 gcgaggctat atatttcagg aagccgagcg acagcgggca ggtcaaatgc tctgcctgct
      181 ggccgtgctg gctgagagtt ttgcagcaat tctggtcttt acgaccatca aaaatcccat
      241 cgacgccgct ttgatcttct tgagcgccct tttgggtgcc ggcttcatta aggtcactct
      301 gggagcccgg gaattcattc cctattacag tgagctaatg gccgttctgg agcatactca
      361 taatgccaac ctgagggtaa aagccctgca gttcttcctc aaagtagtga acgggctcct
      421 ctttaccgtc tcctatgcag ccttcgccct ggtgctggct ttcggttcct ttctgctcta
      481 tgtggtgcac gccgtcggcg acctgggtac ctatctggcc cttctggctc aattactgac
      541 acaacttgcc atcctcacct gcttcataga agctcatttg ctctattttc acgacagccc
      601 caccccggaa ggacaggcag ctttgcaggc tcttaaagaa acatacaaag gcaaatccac
      661 catagatacc ttaaaatcaa tgctcacagg taccacctac gaaagcgata tgagttatat
      721 gctcgccatc tatggtcccg agtcgctgct atttctgtag cactgccggc tataccagtg
      781 tttttgcgcc accaccaata gagaaacaat aaaatcaaat gaatcttcct cagggtatga
      841 cagccataat caccggtgcc tcaaccggta taggcagggg tctggcacta tcacttacca
      901 aaatctacaa ggccaaactg gtcttgacgg caagaagccg ggaagacctg gaaacactgg
      961 cccaggaggt caagcgactg ggcggcgagg ccgtggtgct ggccgccgat attgccggtg
     1021 aaggcgtgac ggagagcctg gtcaatctgg ccgtaaaaaa cttcggcaag ctaaatatgg
     1081 tggtcaataa cgccggcatg ggtatttcag gcagctttgc caaactttcg gaagaagact
     1141 ggcgccgcat cttcgaagtc aattttttca gcgccctcac tctcacccaa catgccctgc
     1201 cgcacttacg cgctgaagaa gtggctaaac tggtcaatat ctcatcggtg gcaggcaaag
     1261 tgtctattcc cggctcagtc agctactgtt ccaccaagtt tgctctcacg gcgctttcag
     1321 aggggctggc ggcagagctg gctcccgata acatcgatgt tctgactgtc tgtcccggtt
     1381 gggtgagaac ggagttcttc cagaaaaacg acctgcccaa tacccgaaac cccactgaaa
     1441 tcgccagacg caacgacctg aaaggcttcc tcatgaagaa tgtgctcagc atcagcaccg
     1501 aggaatgcgt caaggagatc atagaagccc tggagaaagg cggttccaga gagctaattt
     1561 taaccctgcc cggtaaagta gccgaacgcc ttcaaggtac ggcaccaggg gtgatgcgtt
     1621 tcctagccag gcaaattaga ccggaataca aatagaataa aggttctcac ggctgttcag
     1681 gcaactgcac tttcacaaca ctttcaaccg gcgtggtaaa gcggcagaag aaggtgcccg
     1741 gcaagaggtt ggtgagcagg tcctggtaga acttatcata atcctgatca gagaaaaagc
     1801 gaggcttttc actggctccc gcaaataaac tgcgagcagt cggagcttct atacgcaggc
     1861 ccaggctggg aaagattcgc cgccctaatt gattggcatc cctttgcccg accgcaaagg
     1921 atatgagcaa attagcccga tcaagcaaac gctccggcaa atcggcgtgc atgtgcatgg
     1981 gcctgtgcat cgagctgaga ctctgcatag aagccaggta gaaaaggccc ggacagccgc
     2041 aggcatccgc actaaattct atggtgccgg cgtccagggc ccggtcagcg ccgtcaatat
     2101 agaccgataa attactggag atatgaccgg ctactttcag actgactgca aagcggttca
     2161 ggaaacaggc tagataggca gtctgagcct taaatgcatg cag
//
LOCUS       DAOQNZ010000679         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31400_length_2203_cov_2.714618, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000679 DAOQNZ010000000
VERSION     DAOQNZ010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31400_length_2203_cov_2.714618"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="PKW73_12025"
     CDS             <1..1574
                     /locus_tag="PKW73_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74064.1"
                     /translation="LEGRAAAHQQRMSDFMADLDKTAIARDRGRFGAIKDKVTFTNPN
                     TKIEDVVEEMAKNNRAKYPDDAAKQAEITFLKSSGVHNLGDMRGYYLEANLPDMSRFM
                     REAADKGLIAQKSLFTRGKDMVTLSDSQSVRQVLSELKTKGGDPALIASMEAKLPVLE
                     RMGVTNMQQFQVAHAVGTELQSALSLRHRYPQISEITGTTRISELMSRDARAFGENGS
                     ATQQRVDAILGKVPEKPPGMISSTVSGAKNWVFDAAYRDRFWNVGDINRAVQGREARN
                     WGRINPETSGVEAMQRAASSMRARATLAGALTSSVAYNGITSVYDMSYDTDINGNTRK
                     VKVGDEVRQLTPVEAFTRSMVGSGDLTNRIQTSAVADVLLGSMILKPIANPAAQTAKE
                     AWRWAFVSPTLLTRDNDQMGESARLKQTADMMERPLQNEDQSTGTVFEKMQQEQQRQA
                     VPPADANAGKTPEQPKVEPPKVDPAKAEADRKAAEAKAKADREAEEARKRAAQAPKQP
                     NLDPSTMAPGEGFGQ"
     gene            1711..>2203
                     /locus_tag="PKW73_12030"
     CDS             1711..>2203
                     /locus_tag="PKW73_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74065.1"
                     /translation="MVDTPGKVAEVKQEPEKKPPAAEQKFLLETQVPVAKPADRIQVA
                     EDLSIVDNTRLKDAIFKPATAVMGHLADAKAKADEINKLDKEHKDPKDKITVTDASGK
                     AVEMTVDERVKQLKKDIDKQFSDAIKTSQTIPSAPLVELVSNNFAERLSLSQKNGIDM
                     TIGS"
BASE COUNT          596 a          601 c          590 g          416 t
ORIGIN      
        1 ctctcgaagg aagggctgcc gctcatcaac agcgcatgtc cgacttcatg gccgatctcg
       61 ataagacggc aatagcccgc gaccgtggcc gctttggtgc cattaaagac aaagtcactt
      121 tcaccaatcc caataccaag attgaggatg tggtggaaga aatggccaag aataaccggg
      181 ccaaatatcc cgatgacgcc gccaagcaag ctgaaatcac tttcctcaaa tcctccgggg
      241 tacacaacct gggtgatatg cgcggctatt atctggaagc caatctgccc gatatgtcgc
      301 gcttcatgag agaagcggcc gacaagggct tgattgctca aaagtcgctg ttcactcgcg
      361 gcaaagacat ggtcaccttg agcgacagcc aatctgtgcg tcaagttctc tccgaactga
      421 agaccaaggg cggcgaccct gcgcttatcg ccagcatgga agccaagctg cctgtgctgg
      481 agcgcatggg tgtcaccaat atgcagcagt tccaggtggc tcatgccgtg ggcactgagt
      541 tacaatcggc tttgagtctg cgtcaccgtt atcctcagat ttccgaaatt accggcacca
      601 ccagaatttc tgaacttatg agccgggatg cccgggcttt cggtgaaaac ggctctgcta
      661 cacagcaaag agtggatgcc atcctgggca aagtgcctga gaaacctccg ggtatgatta
      721 gctccaccgt cagcggtgct aagaactggg tcttcgatgc agcttacaga gacagattct
      781 ggaacgtggg agacatcaac agagctgttc agggccgtga agcgcgcaac tggggacgta
      841 tcaatccgga gacttccggg gtggaagcca tgcagagagc tgcttcctcc atgcgtgcca
      901 gagccactct ggccggtgcc ttgaccagct ccgtagctta caacggtatc accagtgtct
      961 acgatatgtc ctatgacacc gacatcaacg gcaacacccg caaagtgaaa gtgggtgatg
     1021 aagtgagaca actcaccccc gtagaggcat tcacgcgctc tatggtaggt agcggcgatc
     1081 tgaccaaccg cattcagacc agtgccgtag ccgatgtctt gcttggcagt atgattctca
     1141 agcccatcgc caaccccgct gcccagactg ctaaagaagc ctggcgctgg gccttcgtca
     1201 gtcccacctt gctcaccagg gacaacgacc agatgggtga gtcggccaga ctcaagcaga
     1261 ctgccgatat gatggaacgt cccttgcaaa atgaagacca gagtacaggc actgtctttg
     1321 aaaaaatgca gcaggagcaa caaagacagg cggtgcctcc ggctgatgcc aacgccggta
     1381 agacccctga gcagcctaag gttgaaccac cgaaggtaga cccggccaaa gctgaagcgg
     1441 accgcaaagc agctgaagct aaggccaagg ccgaccgaga ggctgaagaa gccaggaaga
     1501 gagcagcgca agcgcccaag caacctaacc ttgatccatc taccatggct ccgggagagg
     1561 gattcggaca gtagaagcct aaaaagggag ccaaaagctc cctttttact gagaattttg
     1621 acttaatgtg tcgaatttgg ggcatattct gtatagaaca aaacacatcg agggcacagc
     1681 gtcacgattt gttttctagg ggtatgagtt atggtcgaca caccaggaaa ggtagcggaa
     1741 gtcaagcagg agcctgaaaa gaagcctcct gcggcggaac agaagtttct tctggagacg
     1801 caagtgccgg tggccaagcc cgccgaccga attcaagtag ccgaagacct atctatagtc
     1861 gacaatactc gcctcaagga tgccattttt aagcctgcca cagccgtcat gggtcatctg
     1921 gccgatgcca aagccaaggc cgacgagata aacaaactcg acaaagagca taaagatccc
     1981 aaagataaga tcacagtcac cgatgccagc ggcaaagcgg tggaaatgac tgtggatgag
     2041 cgggtcaagc agctcaaaaa ggatatcgat aagcagttct ccgatgccat caaaacttcc
     2101 cagaccattc cttcggcacc actggtggaa ctggtcagca acaacttcgc tgagcgcctg
     2161 tccctgagcc agaagaacgg catcgacatg actatcggct ccc
//
LOCUS       DAOQNZ010000680         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31447_length_2201_cov_3.045666, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000680 DAOQNZ010000000
VERSION     DAOQNZ010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31447_length_2201_cov_3.045666"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKW73_12035"
     CDS             complement(<1..391)
                     /locus_tag="PKW73_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA74066.1"
                     /translation="MTAVPLTLNALISVCLLCWSTWGIFDKKALEAARHIDVLIFQHL
                     FYVLEVPLIFLLLGYLYPQGWSISPPVWGWTLLGSGISSLAMLFYLVAMSRAEASFVL
                     GITAAYPLVVQVFAFFLLHEALVPERVL"
     gene            477..1454
                     /locus_tag="PKW73_12040"
     CDS             477..1454
                     /locus_tag="PKW73_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNA74067.1"
                     /translation="MFRQDPKKAKEKNGPSLKETEVKKPSPTLKEVEEKLSQLELSTV
                     AAASPTALEPVEKKKSLLLGRGFNVFLFWSLVLFSVLFSHAPFLQWLLMPVNQFVVMV
                     HEMSHAFVTVLTGGSVNFMTIVSDGAGHGGLTSSRGGLTFLSAQAGYIGETIFGCLLI
                     YLGQFPHWSRRLLYLMGGIILLGTLLFILPGLLHLQQFFAVIGSLAWALVLAFAFFYA
                     GRKLNDVMANILVLFLAVQCTLNSLSLIWILLPHSLGMTGGGFTDATILAKATFIPAF
                     FWAFSWMVFSILALFFTLKKTYGAFLFGGSGGKAASDKNATAYVKPKKN"
     gene            1506..>2201
                     /gene="ftcD"
                     /locus_tag="PKW73_12045"
     CDS             1506..>2201
                     /gene="ftcD"
                     /locus_tag="PKW73_12045"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNA74068.1"
                     /translation="MTVRLVECVPNFSEGKDRKIIDAIADAIASVSGIRLLDVDPGKS
                     TNRTVMTFVGSPETVVEAAFQGIARAAQLIDMRKHTGEHPRMGATDVCPFVPVTGVSM
                     EDCVKLARELGERVGKELSIPVYLYAEAASKPSRRSLAEIRHGEYEGLEAKMKGADFA
                     PDFGPGSFNAHSGATVIGARHFLIAYNVNLNTRSKKLANEIALNIREAGRAKRDEKGE
                     IVKDANGETVKVAG"
BASE COUNT          475 a          564 c          619 g          543 t
ORIGIN      
        1 ccagtaccct ttccggcacc agggcttcat ggagcaagaa gaaggcgaag acctgcacta
       61 ccagaggata ggctgcggtg atgccgagga cgaagctggc ctcggcccgg gacatggcta
      121 ccagatagaa aagcatggcc aggctggaaa tgccggagcc aagtaaagtc cagccccata
      181 caggcgggct tatggaccaa ccctgggggt agaggtagcc cagaaggaga aagataagcg
      241 gtacttccag cacatagaaa aggtgttgaa agattagaac gtcgatatgt ctggctgctt
      301 ccagcgcttt cttgtcgaag atgccccagg tgctccagca aagcaggcac acggaaatga
      361 gggcgttgag agtgaggggg actgcggtca cggtcttgtg ggtacaccgg gctctgtcgg
      421 tatattatga ataggtatag attagcagtt cgcttggcaa attcaggtag aaagaattgt
      481 tccggcaaga ccctaagaaa gctaaggaaa aaaacggtcc ttctctcaag gagacggagg
      541 ttaagaagcc ttcccctact ctcaaggaag tagaggagaa actctctcag ttggagttga
      601 gtactgtagc ggccgcctct cccactgctc ttgagccggt ggagaagaag aaaagtttgc
      661 tcctggggcg gggctttaat gtctttctct tctggtccct ggtcttattc agtgttttgt
      721 tttcccatgc gcccttcttg cagtggctgc tcatgccggt caatcagttc gtggtcatgg
      781 tgcatgagat gagccatgcc tttgtcacgg tcttaaccgg cggctcggtc aattttatga
      841 ccattgtttc tgatggggcc ggacacggcg ggctcaccag ttccagaggc ggtctgacct
      901 ttctcagcgc ccaggccggc tatattggcg aaaccatttt tggttgcttg ctcatttatc
      961 tgggccagtt cccccactgg tcgaggcgct tgctttatct aatggggggt atcatcctgc
     1021 ttggcaccct ccttttcatc ttgccggggc tcttgcacct gcagcagttc tttgccgtga
     1081 tcggcagcct ggcctgggct cttgtgctgg cttttgcctt cttttatgcc gggcgcaaat
     1141 tgaacgatgt catggctaat atcctggtgc tctttctggc ggtgcagtgc actttgaatt
     1201 ccctttcgct gatctggata ctgctgcccc attcacttgg catgaccggc ggcggcttca
     1261 ccgacgccac aatactggcc aaagctactt tcataccggc tttcttctgg gccttctcct
     1321 ggatggtctt ttcaatactg gccctgtttt ttactctcaa gaaaacctac ggtgcctttc
     1381 tctttggcgg ttcgggcggc aaagcggcct cagacaaaaa tgctactgct tatgtaaagc
     1441 ctaagaaaaa ctgatattgc cttcccggcg ctatataact gaggggtctt ccgggagggt
     1501 tttctgtgac agttcgtctt gttgaatgcg tgcccaattt cagcgaagga aaagatcgca
     1561 aaattatcga cgccatcgcc gatgccattg cttcagttag cggtattcgc ttgctcgatg
     1621 tggatcccgg caagagcacc aatcggacag taatgacctt tgtgggttca ccggagaccg
     1681 tggtggaagc ggctttccaa ggcattgccc gggctgctca gttaattgat atgcgtaaac
     1741 atacaggcga acacccgcgc atgggtgcca ccgatgtctg cccctttgtg ccggttaccg
     1801 gtgtttccat ggaagactgt gtgaaactgg cccgggaact gggggagcgc gtgggcaagg
     1861 aactctccat tccggtttac ctatatgcgg aagccgcgtc caagccatcg cgccgcagtc
     1921 tggctgagat tcgccacggc gagtatgagg ggcttgaggc caagatgaag ggggccgact
     1981 ttgctcccga cttcggaccg ggctcgttca atgcccatag tggtgccacc gtcattggtg
     2041 ccagacattt tctcatcgct tataacgtca acctcaatac ccgctctaag aagttggcca
     2101 atgagattgc cctcaacatt cgcgaagccg gtcgagccaa gcgcgatgag aaaggtgaga
     2161 tagtcaaaga tgcaaacggc gaaaccgtca aagtggccgg t
//
LOCUS       DAOQNZ010000681         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31506_length_2199_cov_3.851679, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000681 DAOQNZ010000000
VERSION     DAOQNZ010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31506_length_2199_cov_3.851679"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKW73_12050"
     CDS             complement(<1..586)
                     /locus_tag="PKW73_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNA74069.1"
                     /translation="MKRTHTGSTLIYGLAAFLAVALIFGFITLNYQQLFGYHKQAQNA
                     VDAAAIAVAKDVQKVTILDPRLGRIGVVDQYGTAGLEQRPILSINTVLATARLDALVA
                     QQLNNTTIMAAATQDLQNAKAAARNLANAVNNYIRGGTAQDADGRTINGGSLLAVAAN
                     AYNVNNRRSSRGFKDLTAADFTIEVGDLRSALGAQ"
     gene            complement(753..1208)
                     /locus_tag="PKW73_12055"
     CDS             complement(753..1208)
                     /locus_tag="PKW73_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74070.1"
                     /translation="MSEENNAEVVQNPSDEGKRISNWAAFVESCAYVGKASRDPSIQV
                     RAGDKLSATLLKGAIFGAFLFLAVPCFGPKEGAIWCYLIADLLFFAATILFVVSRFGV
                     LRVMSLRHALVCWQLMIGTSLLSVALAVNLVFVIVILIAGRYIAQLTPQ"
     gene            complement(1284..1817)
                     /locus_tag="PKW73_12060"
     CDS             complement(1284..1817)
                     /locus_tag="PKW73_12060"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNA74071.1"
                     /translation="MQGIPGRRRASKHGVSARLGRGQQLVELAAGLLVLIPIFLLIID
                     MVVFYLAATLNDNACRDAARAASVVQPVTADGSGNLPASSPIRQRADSVVRKMQHLDG
                     YIIGPRLVNVSLSPDFKRPTNLLLGGNFEGSVEVRTQVDYKLPATIPGILPQQVTLTS
                     QQSFPLTAVLGPQIVPL"
     gene            complement(1911..>2199)
                     /locus_tag="PKW73_12065"
     CDS             complement(1911..>2199)
                     /locus_tag="PKW73_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74072.1"
                     /translation="RVADQIVGGPMGVFGGITPRTSAGFNLRTFRLPDGGNPEPFDVA
                     PGGNLAINQSLYSYTYVVEANYSIQPVLFPFAFAARAQSTCAVEHPEGIGL"
BASE COUNT          546 a          591 c          595 g          467 t
ORIGIN      
        1 cctgcgcacc gagggcggaa cggagatcgc ccacttcaat agtgaagtcg gcggctgtga
       61 gatctttgaa gcctctggaa gaacgtctat tgttcacatt gtaggcattg gcagccacgg
      121 ccagaagact gccaccattg atggtgcggc cgtcggcatc ctgggctgtt ccgccacgaa
      181 tgtagttatt taccgcattt gccaggttac gagcggcagc tttggcattc tgcaggtctt
      241 gtgtggcagc ggccatgatg gtggtgttat tgagctgttg cgcaaccaga gcatcaagtc
      301 gcgccgtggc caatacagta tttatagata ggattggtcg ctgctctaag ccggccgtgc
      361 cgtactggtc taccaccccg atgcgcccca ggcgcgggtc gagaatggtg actttctgca
      421 cgtctttagc tactgcgata gcggcagcgt ctacagcgtt ctgtgcttgc ttatggtagc
      481 caaacagctg ctgatagtta agggtgataa aaccaaagat caaggcaaca gccagaaagg
      541 ccgccaaccc atagatcaag gtactccccg tgtgggttct cttcatgatg ggaagttctc
      601 caaaggcacc taatgccacg gggagcaaaa tggaggctcc ccgagtagta tgtgaaacat
      661 ttttaacaat aatccaaaac ccccaatatt gcaatggtgt ttttacaccc caggggcatt
      721 ctgagattaa cttattctcg ccagaccggc ttctactgcg gcgttagctg cgctatatag
      781 cgaccggcta taagaatgac aataacgaaa accaggttta cagccagcgc taccgagagc
      841 aaactggtcc cgatcatcaa ctgccagcaa accagggcat ggcgcaaaga cataaccctt
      901 aagacaccga aacggctcac cacaaagagt atggtggcgg cgaagaagag caagtcggct
      961 attagatagc accagattgc cccttccttc ggtccaaagc aaggtacggc caggaaaaga
     1021 aaggctccga agatagcccc tttgagcaag gtagcagata gcttgtcccc ggcgcgcacc
     1081 tgaatactgg ggtctcttga ggccttgccc acataggcgc aggactcaac aaaagcagcc
     1141 cagtttgaaa tacgcttccc ctcatcggat gggttctgaa ccacttctgc gttattttct
     1201 tcagacatac ataacctttc taactaattt taagagtagc tatcgggaac cagagcggcg
     1261 gtgcggcaga gcttgcctat gcctcacagg ggaacgattt gaggaccaag cacagccgta
     1321 agaggaaatg actgctggga agtaagggtc acctgctggg gcaagatacc ggggatggta
     1381 gccggcagtt tgtagtcgac ctgggtgcga acttccacac taccttcgaa gtttcctccc
     1441 aaaagcaaat tggtgggtcg cttaaaatca ggcgagagac tgacattaac cagcctgggt
     1501 ccaatgatgt agccgtccag atgctgcatt ttgcgcacca ccgaatcagc cctctggcgg
     1561 attgggctag aggcgggcaa attgcctgat ccatccgcag tcacgggctg caccactgag
     1621 gcggctctgg cggcatctcg gcaagcattg tcgttcaagg tggcggccag ataaaagaca
     1681 accatatcaa tgatgagcag gaagatgggg atgagcacca gaagaccggc ggccagttcc
     1741 accaattgct gcccccgccc gaggcgagcg gatacgccgt gctttgacgc tcttctcctg
     1801 cctgggatac cttgcatatt ctctcgccct ctttcgcctt cagcagtatc ggcgctaaac
     1861 ccgttttaac cgactattct cacttacccg aaaccaaatg taaaacccgg ctagagacca
     1921 ataccctccg ggtgttctac cgcacaagtg gactgagccc tggccgcgaa ggcaaagggg
     1981 aagagcaccg gttgaatgga ataattggct tcaaccacgt aggtgtatga atacaggctc
     2041 tggttgatgg ccagattacc gcccggtgca acgtcaaaag gctccggatt gccgccgtcg
     2101 ggcaggcgaa aggtgcgcaa gttgaagccg gcggacgtac gaggcgtgat tccgccaaat
     2161 acacccatgg gtccaccgac tatctggtct gccactctc
//
LOCUS       DAOQNZ010000682         2198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31536_length_2198_cov_3.374242, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000682 DAOQNZ010000000
VERSION     DAOQNZ010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31536_length_2198_cov_3.374242"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            463..1389
                     /locus_tag="PKW73_12070"
     CDS             463..1389
                     /locus_tag="PKW73_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74073.1"
                     /translation="MVNRFGSPELVSIARQCLDEKVWSEETSYYLPRLLKFDSHRNNR
                     RRFKVWYKYNPEQRTSPRELSVWLEETGCGRRVLSAARIALARDRLSNALILDVLHRF
                     RKRRIVRSWFNKYLRTNIADSSVRKYLVHRMAENPTALEVKLAMSSLKLGTPLEALSL
                     LAKVVSGSKDRTAHLIARDSVVRFPNGPMAFSMARSLMGTHPDFARPWLLEWIKTAED
                     GHRCEALTAILMHAPDAETLAMARSIAEREIATPGSIPASELAGLLTILLKLQADSQL
                     ITLARSLLLKSGDVGLEHQKRLCRLNRILEKL"
     gene            complement(1466..1969)
                     /locus_tag="PKW73_12075"
     CDS             complement(1466..1969)
                     /locus_tag="PKW73_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNA74074.1"
                     /translation="MSELAVIIDGRKYQVEIKSPPQAGTSAFEVVVNGEKKLIEVPGW
                     DQNIVEWHWFEIEGRSYEVDIDQELNWVKSPNGLHRIEVQDIRAGKPARPASRDNRVK
                     APIPGLITAVFVEAGQKVEAGQPLLILEAMKMENEINAPVTGVVSKLNAHVGKSALLN
                     EVLAEIE"
     gene            complement(1984..>2198)
                     /locus_tag="PKW73_12080"
     CDS             complement(1984..>2198)
                     /locus_tag="PKW73_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74075.1"
                     /translation="AVVCDQEVMSGEYNSDTGKRLAQAISVSTAASDDLLALVGAAFY
                     MRRRSHAVAKPARLSGGWYEASRAID"
BASE COUNT          535 a          590 c          528 g          545 t
ORIGIN      
        1 cttgctgaag ctacaattga gcaggacccg cttaaccaat ggagttcaac gctcttgata
       61 gttctcttgc agggcagaaa cgctctggca aggcgagagt ggtttgagga tcttctaaag
      121 aaaaaggggc tcactttacc agactcgaca tcatccgact cagagccaat agaacagcga
      181 gaagatctag cagagccttc tactgcctcg ctaattctag aaattgaagc tggcggcttt
      241 ccttctctga aattactctc gactgcatcg aagatggctc ttttcgatga attacgaagg
      301 cgagtacaat ccgctccgga cggacggcac agccatacat atctgtctta tctactacat
      361 tttgacaatc tggaggaaca ccagaatgag ttactggaat tagctgaaaa gtggtgtatt
      421 gaaagacatg gcgatgaacg atcggcagag gtcattgcat tcatggtaaa tcggtttggc
      481 tcgcccgagc ttgtttcaat cgccaggcag tgcctcgacg aaaaagtgtg gtccgaggaa
      541 accagctatt atctgccaag acttctcaaa ttcgacagcc atagaaataa cagaagacgt
      601 ttcaaggtct ggtacaaata caaccctgag caaaggacgt caccgcggga actatcagtc
      661 tggctggaag aaactggttg tgggcgccgt gttctaagtg cagccagaat tgcactagct
      721 cgcgatcgac tctcgaacgc tctcattttg gatgtgctgc atagattcag aaagcgccgc
      781 atcgtaagaa gttggttcaa taagtacctg aggacaaaca ttgccgatag ttctgtaaga
      841 aagtatcttg tacaccgtat ggcagagaac cccacagctc tggaagtaaa attagccatg
      901 tcctccctta aattgggcac gccgctggag gctctctctc tgctcgctaa agtagtgagc
      961 ggttccaaag atagaaccgc tcacctgatt gccagagatt ccgtggtgag atttccgaac
     1021 ggtcccatgg ccttttccat ggctcgttct ctcatgggca cccatcctga ctttgccaga
     1081 ccctggcttc tggagtggat caagacggcc gaggacggac ataggtgcga ggctctcact
     1141 gcaattctca tgcatgcccc tgatgcggaa actttggcca tggccaggtc tattgcagag
     1201 cgagaaattg cgactccagg ctcaattcca gccagtgaac tggccggatt actgacgata
     1261 ctcttaaaat tgcaagcaga ctcccaattg attactctgg cgcgctctct cttgctcaag
     1321 tcaggcgacg ttggtctgga acatcaaaaa cgtctatgta gacttaatag aattctcgaa
     1381 aaattatagg accgttttcg cggcccactt cgaccagtac gaaccccgac aggccagtgc
     1441 gcccgcagcc catttcgacc tatacctatt caatttcagc cagcacttca ttcaatagcg
     1501 cagacttgcc cacatgggca ttcaattttg ataccacacc ggtcaccggt gcgttaatct
     1561 cattttccat tttcatggct tccagaataa gaagcggctg accggcttct actttctgcc
     1621 cggcttcaac gaacacggcg gtgattagcc cgggaatcgg tgccttgact ctattgtctc
     1681 gactggccgg gcgcgccggc ttaccggcgc ggatgtcttg cacctcgatt cgatggaggc
     1741 cgttcgggct tttcacccag ttcaattcct gatcgatgtc tacttcgtaa gagcgtcctt
     1801 cgatttcaaa ccagtgccat tcgactatgt tttgatccca gcccggcact tcaatcaatt
     1861 tcttttcgcc gttgaccaca acttcaaagg cggatgtgcc ggcctgcggt ggagacttaa
     1921 tctctacctg gtacttcctg ccgtcgataa taactgctaa ttcactcatt actattctcc
     1981 gctttagtcg atagcacgac tggcttcgta ccatccgccc gaaagacgcg ccggtttagc
     2041 gacggcgtgg gagcgcctgc gcatatagaa ggctgccccc accagagcca gaaggtcgtc
     2101 actagcagcg gtggaaactg atattgcctg cgccagccgc ttgccggtgt cgctgttgta
     2161 ctcaccgctc atcacttctt gatcgcagac caccgctt
//
LOCUS       DAOQNZ010000683         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31562_length_2197_cov_3.671802, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000683 DAOQNZ010000000
VERSION     DAOQNZ010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31562_length_2197_cov_3.671802"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKW73_12085"
     CDS             complement(<1..354)
                     /locus_tag="PKW73_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74076.1"
                     /translation="MEPLRVALMTDKAKEPQDQIIRQPEVKPLASSDAQTIATPSALD
                     KQTAETDRTNRSSGSLTELALKSRGMTREQVAAQLEAQGDSVSIDFGNGQEASRKSGL
                     TEASLKKTADDSSEST"
     gene            606..863
                     /locus_tag="PKW73_12090"
     CDS             606..863
                     /locus_tag="PKW73_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HNA74077.1"
                     /translation="MHTTNLRKVGGSVMLTVPPALLEQMHLQAGASVSITLEGARLIV
                     EASSKPRYTLAQLLAASDYTTPQPASAREWVDGSAAGRELI"
     gene            860..1186
                     /locus_tag="PKW73_12095"
     CDS             860..1186
                     /locus_tag="PKW73_12095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNA74078.1"
                     /translation="MNRGDIYLVDLNPSQGHEQQGRRPVLVISPSIFNSTTGVPVVLP
                     ITNGGAFAERIGFAVEITGIKATGIVRCDQPRAIDIAARNGLQIDTLPQTILEDVMAK
                     VITIFE"
     gene            1684..>2197
                     /locus_tag="PKW73_12100"
     CDS             1684..>2197
                     /locus_tag="PKW73_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA74079.1"
                     /translation="MAKGEDHDYPPARDQRPASERKPEPEAVRQLEPADAPQSDLELE
                     DEITLSPPGGEPKALTYKSGQVVGNDYILDELLGRGGMGVVFAARHRFIDQKYAIKIL
                     APELLTERNWLRFQREAQSLAKLNHESIVRIFNMGIDKESCPYYVMEFLKGETLADRL
                     HRLGPMSVEEA"
BASE COUNT          494 a          568 c          581 g          554 t
ORIGIN      
        1 cgtgctctcc gaactatcat ctgcggtctt tttcagcgag gcttcagtta gccctgattt
       61 gcgagaagcc tcctgaccgt tgccaaaatc tatgctgaca ctatcgccct gagcttccag
      121 ttgtgcagcc acctgctctc gggtcattcc tcggcttttc aaagctaatt ccgtgagtga
      181 tccgctgctc cggtttgtgc ggtcggtctc cgccgtttgt ttgtccaggg cgctgggagt
      241 agctattgtt tgagcatccg aagaagccag aggctttacc tctggctgtc gtattatctg
      301 gtcttgtggc tcttttgctt tgtcggtcat gagggcgacc cgaagcggtt ccatacccat
      361 tttatgcctt ggcagtcggt ctgataaacc atcggttgat agcggtggcg tctgtcttgg
      421 gcgtttccag ctgaaagaaa acagaaaggt ggctctcgca ctgccgcttg ccggatgcca
      481 ccagattcta tttggctatt caaagacagc cgggttggcg tatgcatgcg gtgccggttg
      541 ttgaaaagac cttggcaaaa ggcgcccagg cggttatact tttgtataac tagtgaggtt
      601 ctgctatgca caccaccaat ttgcgtaaag tcggcggctc cgtcatgctg acagttccgc
      661 ccgctctgct tgaacaaatg cacttacagg cgggcgcctc tgtgagcatc actcttgagg
      721 gcgcgaggct tattgtggag gctagttcta agcctcgcta caccctggct cagctcttgg
      781 ctgcatccga ctacaccact ccccagcctg cctcagcgcg tgaatgggtg gacgggtctg
      841 ccgcaggtcg tgagctgata tgaaccgtgg cgacatttac ctggtcgatc taaacccctc
      901 tcagggtcat gagcaacaag gacgccgccc ggttctagtc atctcgccct ctatcttcaa
      961 tagcactact ggcgttccgg tggtcctacc gattacaaat ggcggtgcct ttgctgagcg
     1021 catcggcttt gccgtcgaaa ttacaggtat aaaagctacc gggatagttc gctgcgatca
     1081 gcctcgggca attgatatcg cagctagaaa cggtcttcag attgacactt tgccgcaaac
     1141 aatccttgaa gatgttatgg caaaagtcat taccattttc gaatgataac cgtgcgtggc
     1201 gaggcgctgc taggtacctt gtaggcatcg cagaactact aaagaaagca ttccagaaag
     1261 ggtataaatt cttgcgcggg aacttattta cactacagaa tgtgttatcg gaaaccaaaa
     1321 tattgagctt gatcgagagc ggagagctag agtttccgca gctcaaattt cggctattga
     1381 agatagattc gcgcgataag gattgagcat gtgatgctgt tcttgaagca gcctggaaca
     1441 aagaaaatga aagtaattcc agtctacctt gcagttctaa cccgttttcg acaagtgagc
     1501 gaaattccgc ataatttcgc tcatgcccca ttttttgaaa tgagaaggtg gtgaccgcct
     1561 tggtcgccat cttgggtgtt ctggaccaaa aacaacgcct attaggcctc ctaccacctt
     1621 aactgagcta tcattccatt tggaagcccg gacccctgtt cccctgaaag gaccgcaacg
     1681 actttggcga agggcgaaga ccatgactat ccgccggctc gagaccagag accagcatca
     1741 gagcggaagc ctgagccgga ggctgtccgg caacttgagc ccgctgatgc gccgcagtcg
     1801 gatcttgaac tggaagacga gataaccctc tctccgccag ggggtgagcc caaggcgctc
     1861 acctataaat ccgggcaggt ggtcggtaac gactacatcc tcgatgaact cttaggacgg
     1921 ggcggcatgg gtgtggtttt tgctgcccgg caccggttta ttgatcaaaa gtacgccatc
     1981 aagattttgg ctccggagct tttgaccgag cgcaattggt tgcggtttca gagagaagcg
     2041 cagtctctgg ccaaactcaa ccatgaaagt atagtgcgaa tcttcaatat gggcatagac
     2101 aaagagtctt gcccctatta tgttatggaa tttttgaagg gcgagaccct tgccgacaga
     2161 ctgcatcgcc ttggccccat gtctgtggag gaagccc
//
LOCUS       DAOQNZ010000684         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31678_length_2193_cov_4.368569, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000684 DAOQNZ010000000
VERSION     DAOQNZ010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31678_length_2193_cov_4.368569"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /gene="rlmB"
                     /locus_tag="PKW73_12105"
     CDS             <1..1304
                     /gene="rlmB"
                     /locus_tag="PKW73_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNA74080.1"
                     /translation="EEGGSGGYEDRKRTYSRPRSEEGGSGGYEDRKRTYSRPRSQEGG
                     GGYEDRKRTYSRPRSEEGSSGGERRPYSRPRSEEGGERRPYSRPRSEEGGETRSYSRP
                     RSDEGGERGRFSNRSREGGTSGFDRGERQFRPRERGAEDLRQFDEEAPPERTRVGRYG
                     SHAVTELPVLDQAEVDAEFVYGRNSVVAFLERGSAPVNKIFIAKDTDPDRRLDKIVAL
                     AKDNGIPTVVCDKGKLSTLIGDRDKHQGVVAQMAASEFVELPDFLDKLDAVKAEYEAR
                     GESMDGYSIAILDGIEDPHNIGAIIRSADAAGVKAILLPQRRSAGLTSTVAKVSAGAL
                     ASMTVVRITNIVATLEKLKDRGFWVAGLSLEGAEDLFKSDLKRPLALVIGSEGDGISR
                     LVAEHCDMLVKIPMAGQVQSLNASVAAGVVFYEIVRQNVKA"
     gene            1453..1524
                     /locus_tag="PKW73_12110"
     tRNA            1453..1524
                     /locus_tag="PKW73_12110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1485..1487,aa:Thr,seq:tgt)
     gene            1529..1611
                     /locus_tag="PKW73_12115"
     tRNA            1529..1611
                     /locus_tag="PKW73_12115"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1563..1565,aa:Tyr,seq:gta)
     gene            1810..1884
                     /locus_tag="PKW73_12120"
     tRNA            1810..1884
                     /locus_tag="PKW73_12120"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1842..1844,aa:Thr,seq:ggt)
     gene            1996..>2193
                     /locus_tag="PKW73_12125"
     CDS             1996..>2193
                     /locus_tag="PKW73_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNA74081.1"
                     /translation="MARQKFERSKPNVNIGTIGHVDHGKTSLTAAITLALSKQGKAKF
                     KKYDEIDAAPEEKARGITINTA"
BASE COUNT          527 a          557 c          639 g          470 t
ORIGIN      
        1 cggaagaagg cggcagtggc ggctatgaag atcgcaagcg cacctactcc agacctcgtt
       61 cggaagaagg cggcagtggc ggctatgaag atcgcaagcg cacctactcc agaccacgct
      121 cgcaagaggg tggcggcggc tatgaagacc gcaagcgcac ttactccaga cctcgctcgg
      181 aagagggtag tagtggcggc gaaaggcgtc cctatagccg tcctcgctcg gaggaaggcg
      241 gtgaaaggcg tccttacagc cgtcctcgtt cggaggaagg cggtgaaacc cgctcttaca
      301 gccgtccccg ttcggatgaa ggtggtgaac gtggtcgctt ttccaatcgc tcccgggagg
      361 gcggtactag tggatttgac cgtggtgaaa ggcagttccg cccgagagag cgcggcgccg
      421 aagacctcag acagttcgat gaggaagcgc cgccggagcg cactcgcgtc gggcgttacg
      481 gcagccatgc cgtgactgaa ctgcctgtgc tcgaccaggc ggaagttgat gccgagtttg
      541 tctacggaag aaattccgtt gtcgcttttc ttgagcgcgg ctctgctccc gtcaacaaga
      601 tttttatcgc taaagatacg gatcccgaca ggcgtctcga taagatcgtc gctctggcca
      661 aagacaacgg cattcccacc gtggtttgcg ataaaggcaa gctctctaca cttataggtg
      721 atcgcgataa gcatcaaggc gtagtggccc agatggccgc cagcgagttt gtagagcttc
      781 ctgattttct cgacaaactg gatgcagtga aggctgagta cgaagcgcgg ggcgagagta
      841 tggacggcta ttccatcgcc attctcgatg gtatcgaaga cccgcataat atcggcgcta
      901 tcatcagaag tgccgacgcc gccggcgtta aggccatcct tctaccgcag agacggtctg
      961 ccggtctgac ttccaccgta gccaaggtaa gtgccggcgc ccttgcttcc atgactgtgg
     1021 tgcgcatcac caacatcgtt gccacacttg aaaagcttaa agacaggggc ttctgggtgg
     1081 ccggattgag tcttgaaggg gcggaagatc tatttaagtc cgacctgaaa cgcccgcttg
     1141 ctctcgtcat cggctctgaa ggagacggta tcagcaggct ggtggctgag cattgtgaca
     1201 tgctggtgaa gattcccatg gccgggcaag tacaatctct caatgcctca gttgcagcag
     1261 gcgtggtgtt ctacgagatt gtcagacaga acgtcaaagc ctgagctggt ttcaaagcga
     1321 agcttcgtta caaaaattat attaagttcc tgtagcgaga ttgagcaatg ccgaggcgtt
     1381 tgtttgacac gaacaaaatc atgtatgtag aatcttaagt tcttgtccgt gcatgggtga
     1441 ccttgcgcgc ttgccggtgt agctcaacgg tagagcaacg gttttgtaaa ccgtaggttg
     1501 cgggttcgaa tcccatcacc ggctttgtgg gcggatgtcc gagtggctaa aggagacggg
     1561 ctgtaaactc gttggcgaaa gcctacactg gttcgaatcc agttccgccc aatttccctt
     1621 ccgggaatgg cttcgagagc aagcgacgtg caaccctggt ggttggtcca atctagactt
     1681 tcgcctagcc agcgatctgt ttagatagga ctgggtccgg cagcagtgcc ggacaatggt
     1741 taagttgcac gtaaaagaga gctcgttcca tatagaacgg attctccggg gcggtaaaat
     1801 atgccctgcg cctatgtagc acagcggtag tgcaccccct tggtaagggg gaggtcacga
     1861 gttcgattct cgtcataggc tccattttta tatgagcgcg tttctagcgt cgcaaatcaa
     1921 ataagaaaac gcgttcagcg cgtgttaggg accaagtaag taagcagtca accaacagtt
     1981 aaggagagtt gtaggatggc ccgccagaag tttgagagaa gtaagccgaa tgtaaacatc
     2041 ggcaccatcg gccacgtcga ccacggcaaa accagtctga cagccgcaat cacactggca
     2101 ttgtctaaac aaggcaaagc caaattcaaa aaatatgatg agatcgatgc cgctccggaa
     2161 gaaaaagccc gtggtatcac catcaatacc gcg
//
LOCUS       DAOQNZ010000685         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31681_length_2193_cov_3.728251, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000685 DAOQNZ010000000
VERSION     DAOQNZ010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31681_length_2193_cov_3.728251"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKW73_12130"
     CDS             <1..386
                     /locus_tag="PKW73_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74082.1"
                     /translation="RWEIFLTRAKMNEDRGSYALAESDYNLLVAESDSHGDSTSSGAR
                     VMRGQFFEKNGKLDKALADYDFCLKKDAGNEEALKSRADIYMKLKRPQSALRDYRKLL
                     EVAGSDRALAEVARAGIARASAAPR"
     gene            complement(391..2085)
                     /locus_tag="PKW73_12135"
     CDS             complement(391..2085)
                     /locus_tag="PKW73_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74083.1"
                     /translation="MSFLALLTTSGSRPCLAKHEFEESGDNFSRYNNHADPDAPTLRH
                     SKPGEPYILTIGFAEDAEEFTQKILKSLKAKNHLTSVKKEGPANVVSDLTTKETFKIL
                     PNRQGKRTASSVTPEFLAEIFPNNSTILFTLSSEYEKQRKWQKALDLYQKAFKLQYGE
                     QLTSRNPGPTERAIQGNYYANCAYFELNLQNRQAALEHLSQAIKLRPEQIANYRNRAN
                     LYMQLGQYDLAKADKQKVLELTRQNQLKKGSPAAAIDEANEPLGQVKRGAYEQAIETC
                     TNIISHDPKYKKAYFARLLAKVHTGDYKGAVEDGSKLAELNNNNANVKQMVKELKMLL
                     KEGPPPYGDLSILDEPPPVLFTRLNGLKAYSMPMSKYAREHKGDARVYDALAVRYWKA
                     GRLKEADDQVNKMLHMDRADLCVLRQKTQTAEALGNWKETREACDLYLKEMGENPEAF
                     LNLDLLQSVYSTRKRANAELKNYGKAIEDCNMLLKMEPDSAEVYRDRADYYMKINNFA
                     QAVKDYSQSIKFDDTKTSSNYLMRAAAYDKLKQTALANADRETARKLDLGLKRSGK"
BASE COUNT          440 a          525 c          608 g          620 t
ORIGIN      
        1 acaggtggga aatttttctc actcgcgcca agatgaatga ggacaggggg agctatgccc
       61 tggctgagag cgactataat ttacttgtgg ccgaaagcga tagccatggg gatagcacca
      121 gcagcggtgc gcgggtgatg cgcgggcaat tcttcgagaa gaacggtaaa ctcgacaagg
      181 cccttgccga ttacgacttc tgcttgaaga aagatgccgg caatgaggaa gctctgaagt
      241 ctcgcgctga tatctatatg aagttaaagc gtcctcaatc ggctttacgc gactatcgca
      301 agctgctgga ggtcgccggc agtgaccggg ctttagccga agtagctcgg gccggcatcg
      361 ccagagccag tgcggcgcca aggtgaaagt ttacttgccg cttctcttca gccccaaatc
      421 tagcttgcga gccgtttcac gatcggcgtt agccagagcg gtttgcttta acttgtcgta
      481 ggcggcggct ctcattaagt agttggagct tgtcttggtg tcatcgaatt ttatggattg
      541 gctgtagtct ttcaccgctt gagcgaagtt gttgattttc atatagtagt cggctcggtc
      601 gcgatagact tcggctgagt ctggttccat ctttagtagc atgttgcaat cttcgatggc
      661 ttttccataa tttttcaact cggcgttcgc tcttttgcgg gtcgagtaga ccgattgaag
      721 aaggtcaagg ttgaggaagg cttccggatt ctcgcccatt tctttcaagt agaggtcgca
      781 ggcttcgcgg gtctctttcc aattgcccag agcttcggcg gtttgagttt tctgcctcaa
      841 aacacagaga tccgcccggt ccatgtgcag cattttgttt acctgatcgt ccgcttcctt
      901 gagtcgacca gccttccagt agcgcacggc cagggcgtcg tagacccggg cgtctccttt
      961 atgttcgcgg gcgtacttgg acatgggcat ggaataggct ttcagtccgt tcaatcgggt
     1021 gaataatacc ggcggcggtt catcgagaat gctcaagtct ccgtagggtg gtggtccctc
     1081 tttcaggagc atctttagct ctttcaccat ttgcttgaca ttggcattgt tattatttag
     1141 ctcagccagc ttgctcccgt cctccacggc gcctttgtag tcgccggtat gcaccttggc
     1201 cagcaagcgg gcgaagtaag ctttcttata tttaggatcg tgggaaatta tatttgtgca
     1261 ggtctcgata gcttgttcgt aggcgccacg tttcacttgc ccgagcggct cgttggcttc
     1321 gtcgatggcc gcagccggtg agcctttctt gagctgattc tgtctggtca attccagcac
     1381 cttctgttta tcggccttag ccagatcgta ttgaccgagt tgcatgtaca ggttggcgcg
     1441 attgcgataa ttggctatct gctcgggcct tagtttgata gcctggctca agtgttcgag
     1501 agctgcctgc cgattttgta aattgagctc aaagtaagcg caattggcgt agtagttgcc
     1561 ctgtatggcg cgctctgtgg ggccgggatt acgactggtc agttgctctc cgtattgtag
     1621 tttgaaggcc ttctggtaaa ggtcgagagc tttttgccat tttctttgtt tctcgtattc
     1681 actggagagg gtgaacaaaa tggtgctatt gttgggaaaa atctcggcta gaaattccgg
     1741 cgtgacacta ctagcggtgc gttttccctg ccgattcggc aaaatcttga aagtctcttt
     1801 agtagtcaga tcgcttacga cattggccgg tccctctttc ttaacactgg tcaggtgatt
     1861 cttggccttg agagatttga gaattttctg ggtaaactcc tcagcatctt ctgcaaaacc
     1921 gatagtgaga atatagggct cgcccggctt ggaatgcctc aaagtgggtg cgtccgggtc
     1981 ggcatgatta ttgtagcgac tgaaattgtc gccgctttct tcgaactcat gcttagccag
     2041 gcatggccgg gaacccgagg tagtaagaag cgccagaaaa gacactagag tcaaaaaggt
     2101 ccggcgcaat ctcatttgtt cggtttgacc tcgctggtat taattcatat tagcaccgcc
     2161 ctggcaccgc cgggctcggc gagcctcaag gtt
//
LOCUS       DAOQNZ010000686         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31738_length_2191_cov_3.303839, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000686 DAOQNZ010000000
VERSION     DAOQNZ010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31738_length_2191_cov_3.303839"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1185)
                     /locus_tag="PKW73_12140"
     CDS             complement(49..1185)
                     /locus_tag="PKW73_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74084.1"
                     /translation="MVEGKTTEERGGQRGDQSGPELGNGKAVQEVAAQRLQAEAAQTA
                     GTAGKGSSTELTIMLKDGATLRNVGRQIYFETLKRLPDEQELKAFVNDTARRNGLKTK
                     DQQGSLEVGQVLKIVPPPPDRTTPGTHGAHKGGQLGDETKRDGKGSTGKTGQDTHVLA
                     GGTKPTDKRHPLMKGGDSCEGEWANMVSQWVTQNEGSRDKHRAFNPNDMGQGISVGLL
                     QWNQKKGKLPDLLHAWHDKNPEKFQTMFGAYSTNLTRDSFVRSADFVGNKTLHDGMVK
                     ALADREFQGVQDTLRNKNMVRACEVATDYGFKSLRGRAVVSDLFNQLGEGGARNVIRK
                     VALDKPESARIEELKRRTSGRINGEDRVSTLEENVKRIWRQLGS"
     gene            complement(1361..>2191)
                     /locus_tag="PKW73_12145"
     CDS             complement(1361..>2191)
                     /locus_tag="PKW73_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA74085.1"
                     /translation="LAFCPCINEPLYQACSVHPCRVEMNLLEPPFMEGFKGEHRFFPV
                     INCQGRSLSLHGVYFRSNSSATYSKHNGKSKGTVIFSLGNSSCLAYMLGTKPMVTLLK
                     LGYDIFAYDYEGFGISQGEANYRKLGNDGLSAFEYVSTKINPENIILYGLSMGTGVSS
                     YIAERKPVKAVILDSPYVTPEKTIKLWFPILSIYPSWMFPEPKYDNARFVSARHPPVL
                     VITKGCDEVTLPTQGLELSKVASQPASTLYLKNSHHCYVSDEEEPLYTSRLKTFLAAL
                     "
BASE COUNT          442 a          569 c          578 g          602 t
ORIGIN      
        1 cccgtcgccg ctcttgttga agcagctgtc cgacctgcca gtctgaccct agcttcctag
       61 ctggcgccag attctcttta cgttctcctc aagggtagag actcgatctt ctccgttgat
      121 gcgaccgctg gtgcggcgtt ttaattcttc gatgcgggcc gattcgggct tgtccagagc
      181 tactttcctt atgacgttgc gcgcgccgcc ttctcccaat tggttgaaga gatcgcttac
      241 aacggctcgt cctctcaatg atttgaagcc atagtcggtg gcaacttcgc aggctcgaac
      301 catgttctta ttgcgcaaag tatcttgcac accctgaaat tctcgatcgg caagtgcctt
      361 gaccatgccg tcatgaagcg tcttgttacc gacaaaatcg gcagagcgaa caaaactgtc
      421 cctggtcagg ttggttgaat aggctccgaa catggtttga aacttttcgg ggttcttatc
      481 gtgccaggca tgaagcagat ctggtagttt tcccttcttc tggttccatt gcaaaagccc
      541 gacggaaatg ccctgaccca tatcgttggg attaaaagca cggtgcttgt ccctggagcc
      601 ttcgttctgg gtcacccatt gtgaaaccat gttggcccac tcgccttcgc aactgtcccc
      661 gcctttcatg aggggatgtc gtttatcggt aggttttgtg ccgccggcaa gtacatgagt
      721 gtcctgaccc gttttgccgg tgctgccctt accgtccctt ttggtttcgt cgccgagctg
      781 tccaccttta tgggcgccat gggttcctgg cgtagttctg tccggcggag gcggcacaat
      841 tttcagcact tgtccgactt cgagactgcc ttgttgatct ttggtcttca ggccgttgcg
      901 tctggctgtg tcatttacga aggccttgag ctcttgctca tccggcagcc gcttcaaagt
      961 ctcaaaatag atttgtctcc cgacattgcg caaagtagcc ccatctttga gcattatggt
     1021 aagttcggtg gatgaccctt tacctgcggt gccagccgtc tgtgctgctt ccgcctgcag
     1081 tctctgcgca gctacttcct gaacggcttt tccgttgcca agctcgggtc ccgattggtc
     1141 gccgcgttgg cccccccgtt cttctgtagt cttgccttca accattgcta cctgcacaat
     1201 cttcttgaaa gatgaggcct attcaatcga accagtctgg taaacagcct atacttttgg
     1261 caatatgccc cggcctggct gagccgaaac ccgcagggac cgaccttaga aagagcactg
     1321 attggactga gagaaggcct ggaccggacg cggagaagac ctagagcgca gccaggaagg
     1381 tttttagtct ggaggtatag agcggctcct cttcatcgct gacgtagcag tggtgtgagt
     1441 ttttgagata cagagtggac gccggttggc tggcgacttt gctcaactcg agtccctgtg
     1501 tcggtagggt gacttcatcg cacccctttg taattaccag cactggtgga tgccgggcac
     1561 ttacaaagcg agcattatca tattttggtt cagggaacat ccaggaggga tagatgctga
     1621 gaatgggaaa ccagagcttg atggtttttt caggtgtcac atagggagag tcaagaatca
     1681 ctgcctttac cggctttctt tcagctatgt agctggacac tccggtgccc atggatagac
     1741 catataggat aatgttttcc gggttaatct ttgtgctcac gtactcaaag gccgataatc
     1801 cgtcgttgcc cagctttctg tagtttgctt cgccctgact gatgccgaaa ccttcatagt
     1861 cgtaggcgaa aatgtcatat cctagtttaa gtagggtcac cattggtttg gtacctagca
     1921 tgtaggccag gcaactgcta tttccgaggc tgaatatgac cgtacctttt gatttaccgt
     1981 tgtgtttgga gtaagtcgca gaactgttgg aacggaagta gacaccatgc agggagaggc
     2041 ttcttccctg gcaatttatt acagggaaga agcggtgctc acccttgaag ccttccatga
     2101 aaggtggctc cagtagattc atttccaccc ggcatggatg tactgagcag gcctggtaaa
     2161 gaggctcgtt gatgcaggga cagaaggcga g
//
LOCUS       DAOQNZ010000687         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31809_length_2189_cov_2.722118, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000687 DAOQNZ010000000
VERSION     DAOQNZ010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31809_length_2189_cov_2.722118"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PKW73_12150"
     CDS             complement(<1..1434)
                     /locus_tag="PKW73_12150"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNA74086.1"
                     /translation="MIQGTSSNAGKSLVAAGLCRILKRRGLSVAPFKPQNMSLNSVVT
                     KDGGEIGRAQATQALACEVDAHSDMNPVLLKPLTDCGAQVILAGKAFKTMNAGDFHEF
                     KLQAKQTVMEAFARLKGQYAVIVVEGAGSPAEINLRQGDIANMGFAEAIDCPVVLVAD
                     IDRGGVFAQIVGTMALLSTSERARINGFIINKFRGDIAILQPGIDWLEKETGKPVLGV
                     LPYLENLYMEAEDSLSQTTNLEEEALAAKHALQKLKVAVLRFPKLSNSTDFDPLALHP
                     QVHLTYIYSGDCLRGFDLIVLPGSRSVAKDLDWLHASGFAAEIRRHLRYGGKVMGICG
                     GFQMLGKVLHDPDALESASARTECLGLLDLETTMLPSKTLTLVSGTLTLDETAVRGYE
                     IHMGITTGKALERPLVKFVNTTDGAISEDEQILGTYLHGIFDDENALHTLLSWSGLQD
                     AAVSDYQMVRMENLDRIADTMEASLNMD"
     gene            1481..>2189
                     /locus_tag="PKW73_12155"
     CDS             1481..>2189
                     /locus_tag="PKW73_12155"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HNA74087.1"
                     /translation="MKIATSPLLVVPALVLDEMLGEPQGWHPLAVFGREAEKIEDFFH
                     PLAREANGNLANKSNSTGNLAKRLRTNLAGLLAVSQCVVPPVVLLCRLRKQGRNIPLF
                     CHLLDVIVLYACIAPSSLREHGMCVHEALVNGSLAEARTAVGRIVSRDTAALNEEGVA
                     VAAVESILENGSDAIFGALFWFAVAGAPGALFYRLVNTLDAMWGYRNERYVDFGWAAA
                     RLDDLLNFIPARLTAFTY"
BASE COUNT          455 a          568 c          601 g          565 t
ORIGIN      
        1 gtccatattg agggaagctt ccatggtgtc agcgattcgg tcaagatttt ccatccttac
       61 catttgataa tcgctgacgg cggcatcttg tagaccggac caggaaagca aggtgtgaag
      121 agcgttttcg tcatcgaaaa tgccgtgcaa ataggtcccg agtatttgtt catcttctga
      181 aattgcaccg tcggtagtgt ttacgaactt tacaagcggt ctttccagag ctttgccggt
      241 tgtaattccc atatgaattt cgtagccgcg cactgctgtt tcgtccaggg tgagagtgcc
      301 gctgacaagg gtgagggtct tactgggtag catggtggtt tcaagatcaa gaagacccag
      361 gcattcggtt ctggcgctgg cgctttccag ggcatccggg tcatggagga ctttccccaa
      421 catttgaaaa ccgccgcaga tgcccatgac ttttccgcca taacggagat ggcgtctaat
      481 ttctgctgca aaacccgagg cgtggagcca gtccagatct ttggccacgc ttcttgagcc
      541 gggcaggacg attaaatcga atcccctgag acagtcgccg ctgtatatgt aggttaagtg
      601 cacttgcgga tgaagagcca gagggtcgaa gtcggtggag ttgcttaact tgggaaagcg
      661 caaaacagct actttcaact tctgcaaggc gtgcttggca gctagcgctt cctcttcaag
      721 attggtggtt tgactgaggc tatcctcggc ctccatataa agattttcca gataaggcag
      781 cacccccagc accggtttgc cggtttcctt ttccagccag tctatacccg gctgcaatat
      841 ggcgatgtcg ccgcgaaatt tgttgatgat aaaaccattg atgcgagccc gttctgaagt
      901 tgaaagcagg gccatggtgc ccactatttg agcaaagacg ccgcccctgt cgatgtcggc
      961 cacaagtacc accgggcaat cgatcgcctc ggcaaagccc atgttggcaa tgtcaccctg
     1021 gcgtaagtta atctcagccg gactgcctgc accttctacc acaatcacgg catactgccc
     1081 tttcaatctt gcaaaggcct ccatgacggt ttgttttgcc tgcagtttga attcatgaaa
     1141 gtcgccggcg ttcatggttt taaaagcctt gccggccagt atgacctggg cgccgcagtc
     1201 ggtcaggggt tttaaaagca ccggattcat gtcgctatgg gcatctactt cgcaggcgag
     1261 cgcctgggtt gcctgggctc gcccaatttc gccgccgtct ttggtgacta cgctgttcaa
     1321 agacatattt tgtggtttga agggcgccac gctcagcccg cggcgcttca agattcgaca
     1381 caagccggcc gccacgagac tcttgccggc gttggaactg gtaccttgta tcattaatgt
     1441 gtttttcagc atagcaatca gggattcttt cacgccgccg atgaaaattg ctacatcccc
     1501 gctcttggtt gtgccggcgc tcgtgctgga cgaaatgctt ggtgagcctc aaggttggca
     1561 cccccttgca gtttttggca gagaggcgga aaaaatagaa gatttctttc atcctttggc
     1621 aagggaggct aatggcaatc tcgccaacaa atctaactcg accggaaatc ttgccaaaag
     1681 actgagaacc aatcttgccg gactcctggc tgtgagccag tgcgtggtgc cgccggtggt
     1741 gctgctctgc cgcttgcgca agcaaggtag aaacattccc ctgttctgtc atcttcttga
     1801 tgtgattgtt ctttacgctt gcattgctcc cagcagtctt cgcgagcatg gtatgtgcgt
     1861 gcatgaggct ctggtaaatg gttctctggc tgaggccaga acagccgtag gtcgcatagt
     1921 cagccgtgac accgccgccc tcaacgagga gggtgtggcc gtggccgcag tggagtcgat
     1981 tctggagaac ggcagtgatg ccatttttgg tgccttgttc tggtttgcgg tagccggtgc
     2041 gccgggcgcg cttttctatc gcctcgtaaa tactcttgat gctatgtggg gttatcgcaa
     2101 cgagcgctac gtggactttg gctgggcggc tgcgaggctt gacgatctgc tcaactttat
     2161 accggccaga ctgaccgctt ttacctatg
//
LOCUS       DAOQNZ010000688         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31832_length_2188_cov_3.691514, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000688 DAOQNZ010000000
VERSION     DAOQNZ010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31832_length_2188_cov_3.691514"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="lnt"
                     /locus_tag="PKW73_12160"
     CDS             <1..724
                     /gene="lnt"
                     /locus_tag="PKW73_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNA74088.1"
                     /translation="VSLFTKYLYLCDQCPPESICIWPEWSMPVNFSSTEGTLPQLAAL
                     AAKNRQAWVVGIRDTDSQGRGFNATCAVDKNGALLPQVYYKQHLVPMGEYVPDWIERT
                     PMRYLFFGTGSHYPRVNCGDKTVVFKLQDAGVGSLICFENAFPKLSCKAVRAGAEILA
                     DSSDNAWFKRSILSDQMISFSVFRAAENNRPFVFATALGPSVIVDARGRILKRSGKDC
                     ADVISAPVTIEPAPSLFSRWCF"
     gene            complement(857..>2188)
                     /locus_tag="PKW73_12165"
     CDS             complement(857..>2188)
                     /locus_tag="PKW73_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74089.1"
                     /translation="AATKVAALAGQNGACVDIASTNLAAAGYLTYIATALLLLFLLPY
                     MMLKRGVKPVYTFGLLTLINMPIWYWARPQLVSFFMLPMFILLLEDFRRNGYRKRLWL
                     LPVLLIVWVNSHAFWFMGLALILAYLAPQVVSPKSVDYKHRRHLVGILATSCLAPLAN
                     PYGADLVRHTLSFGSEPDFGQISELQPTLLTNPLGHRGIWLYLVIAWSVLLARRAVIP
                     LTGLFFCALSTFTGVVCYRFAAVAVLVSWSYLSIALGKGQQGSEGSTSKTDGSEIKAK
                     ESAIKTVSPVSTALALIAPIFATAVYFNYCQSQKQIWFTHNDSNQKVTAFLKQNSQLR
                     SRLFCDAALGCSLILEDLTPVFIDTRFDLYGKTFCREYQDCLNGSVTDTFNWRSYLDK
                     WQINTLAIKNSAPLNNELNHAARQGSCKKLYDDGIYSVWEIERGAKAGASQ"
BASE COUNT          456 a          588 c          636 g          508 t
ORIGIN      
        1 cgtgagcctt ttcaccaaat acctctatct ttgcgatcag tgcccgcccg agagcatttg
       61 catctggccc gagtggtcga tgcctgtcaa cttcagctcc accgagggca ctctgccgca
      121 gttggcggcg ctggcagcta agaaccggca agcctgggtg gtgggcattc gagacactga
      181 cagccagggg cgcggcttca acgccacttg cgccgtcgat aaaaacggtg cgcttttgcc
      241 gcaagtttat tacaagcagc acctggtgcc catgggcgag tacgtgcccg actggattga
      301 gcgcaccccc atgcgctacc tcttcttcgg caccggaagc cattatccaa gagtaaattg
      361 cggcgacaaa accgtggtat tcaaactgca agacgctggc gtcggctccc taatttgctt
      421 cgagaatgcc tttcccaagt tgagctgcaa ggcggtgcgc gccggggcgg agattctcgc
      481 cgacagcagc gacaatgcct ggtttaagcg gtcgattttg agcgaccaga tgatttcgtt
      541 cagcgtattt cgggcggctg agaacaatcg ccccttcgtc tttgccaccg cccttggacc
      601 ctcggtgatc gtggacgccc gggggcgcat cttgaagcgg tccggcaaag actgcgccga
      661 tgtcataagc gcgccggtca ccatcgaacc ggcgccgtcg ctattcagcc gctggtgttt
      721 ttgaccgtag gctcctttaa ctgctggttc ttttaactgc tggatctttt aaacgtcgac
      781 tcttttcgcc gccggtgctt ttaagcgtag gctcttttca ccgctggttc tatcaagcgt
      841 cggcgcttcg gtttttctat tggctcgccc cagccttagc cccgcgctca atttcccaca
      901 ccgaatatat gccgtcatcg tagagcttct tgcagcttcc ctgcctggcg gcgtggttga
      961 gctcattgtt caggggcgcg ctgtttttaa tcgccagtgt gttgatttgc catttgtcca
     1021 gataactgcg ccagttgaag gtgtcggtga ctgaaccatt gaggcagtcc tggtactcgc
     1081 ggcagaaggt tttgccatag aggtcgaacc gcgtatcgat gaataccggc gtcaggtctt
     1141 ccagtatgag actgcaacct agcgccgcgt cgcaaaagag gcgcgacctg agctggctat
     1201 tttgcttgag aaaggcggtg accttctggt tgctgtcgtt gtgggtgaac catatttgct
     1261 tctggctctg gcagtaattg aagtagacgg cggtggcgaa gatgggggca atcagggcca
     1321 gggcggtgga gaccggtgaa accgttttga ttgcgctttc cttcgcctta atttcgcttc
     1381 cgtccgtttt gcttgtgctc ccctccgaac cctgctgccc tttgcccagg gcgattgata
     1441 agtaagacca ggagacgagc actgctacag ccgcaaatcg gtagcacacc acaccggtga
     1501 aggttgagag ggcgcaaaag aaaagcccag tgagaggaat cacggctctt ctggctagca
     1561 gcacagacca ggcgatgact aagtagagcc agatgccgcg gtgccccagg gggttggtga
     1621 gcaaggttgg ttgcagttct gatatttgcc cgaagtcggg ctcgctgccg aaggagagcg
     1681 tgtgtctcac caggtcggcg ccatagggat tggctaaagg tgccaggcaa cttgtggcga
     1741 gaattcctac aagatgccgg cgatgcttat aatcgaccga ctttggactc accacttgtg
     1801 gtgccaaata cgccagtatc agtgccagac ccatgaacca gaacgcatgg ctgttcaccc
     1861 aaacaattag aaggacgggt agcagccaga ggcgttttct ataaccattc cggcgaaagt
     1921 cttccagcaa aagtatgaac atcggcagca tgaaaaatga cactagctgc ggtcttgccc
     1981 agtaccaaat tggcatgttg atgagcgtaa gcaacccgaa tgtgtagacc ggtttgacgc
     2041 cccgtttgag catcatatac ggtagcagga agagcagcaa aagcgcggtg gcaatatagg
     2101 tgagatagcc tgcggcggcg agattggtag aggcgatgtc gacgcaggct ccgttttgac
     2161 cggcgagtgc cgccactttg gttgctgc
//
LOCUS       DAOQNZ010000689         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31839_length_2188_cov_3.244257, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000689 DAOQNZ010000000
VERSION     DAOQNZ010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31839_length_2188_cov_3.244257"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKW73_12170"
     CDS             <1..189
                     /locus_tag="PKW73_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74090.1"
                     /translation="SYRQHSPGGNLNPALYSNLNANHYSGQYSSQFANPYANNSTALR
                     RAVGERAINSAPVYGNQF"
     gene            complement(429..1709)
                     /locus_tag="PKW73_12175"
     CDS             complement(429..1709)
                     /locus_tag="PKW73_12175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNA74091.1"
                     /translation="MKNHKHSNKYSGPDPSVDQASIDRQLRINWIRTIFGIALMGGLS
                     FAGLVFLGVNFTVATVILFFFSVLMPLFGWWNSANLVLKMMRCRPPNMNDRDHARLVR
                     LVDEIYPLTGLKKKPEVFISPLPIPNAFATGRSPSTAFIAATEGLFAVGLEDREIKAI
                     LAHELAHVKSRDVAITSLVAVLGSLFSILLAGFAPGMFNACFSERNNREDLVGKLSNK
                     VKRDKKRFADPASAVTGFFITLVVFYIASIFAKLITLFVSRSRESAADVLAANWTRDP
                     CALATALQKIVDWMNRNKAALQLKILLGGMEPLLFVSLHEGEEGLNPPPPKGWLARLR
                     KWVGHLGDNHPPVEDRVTLLDGLAGSVCPRMHDIRADEAKRRRERIREERQRLNSKSA
                     PPAVPPAVPPTASEEGQEGTADEGSETKPGQDPK"
     gene            1968..>2188
                     /locus_tag="PKW73_12180"
     CDS             1968..>2188
                     /locus_tag="PKW73_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74092.1"
                     /translation="MTYSVSHGWKWRWRRVTGGIGNGGLKEILLRAEKSELKDTTKNA
                     LEKLYSGFSRPCIFDEIHLRLENLGEALAL"
BASE COUNT          500 a          580 c          647 g          461 t
ORIGIN      
        1 agctaccggc agcactcccc cggtggcaac ctgaatccgg ctctttattc aaatttgaat
       61 gcaaatcatt actccggcca gtattccagt caatttgcca acccttacgc caataacagt
      121 actgctttgc gccgcgccgt cggcgaaaga gcaatcaata gcgcccctgt ttacggaaac
      181 cagttttgac cgccttccaa taactaagta agctagaagt gacgttgttg gcgggatctc
      241 ttgacaactt gaagcaaaaa aagaacccgg gcggtctgga aatcaagagg ccttcaggct
      301 tccgacttcc cgagccaacc cgagttcgaa gtatgcaagt gagtttgtta accgttaacg
      361 gttaagcact cacgactgga actgaatggc aaccggttca agcattgctg ctcaaaccgg
      421 tgccgaattc acttgggatc ctgaccaggt ttggtttcgg acccttcgtc tgcggtaccc
      481 tcttgcccct cttcggaggc ggtgggaggc acggcagggg gcacagccgg cggagccgac
      541 ttgctgttga ggcgctgacg ctcctcgcgg atgcgttcgc ggcggcgctt tgcttcatcg
      601 gcgcgaatgt cgtgcatgcg cgggcagacc gagccggcca gaccgtcgag caacgtgacg
      661 cggtcttcca ccggcgggtg attgtcgccc agatggccca cccacttgcg caggcgtgcc
      721 agccaaccct tcggaggcgg cggattcagc ccttcctcgc cctcatgcag gctgacgaag
      781 agcagcggtt ccatgccgcc cagcagaatc ttgagctgaa gcgccgcttt gttgcggttc
      841 atccagtcca cgatcttctg cagagcagtg gccagagcgc aggggtcccg ggtccagttg
      901 gcggccagca cgtcagcggc actctcgcgg ctgcggctca cgaagagcgt gatcagcttg
      961 gcgaagatgc tggcgatgta gaagaccacc agggtgatga agaagccagt gacggcgctg
     1021 gccgggtcgg caaaacgctt cttgtcgcgc ttcaccttgt tggacagctt gcccaccagg
     1081 tcttcccggt tgttgcgctc gctgaagcag gcattgaaca tgccgggcgc gaagcctgcc
     1141 agaagaatgg agaagagcga acccagaact gccaccagtg acgtaatcgc cacatcgcgg
     1201 ctctttacgt gggccagctc gtgagccaga atcgccttga tttcacgatc ttccagaccc
     1261 acggcgaaaa gaccttccgt agccgcaatg aaagcggtgg agggggaacg gccggtggcg
     1321 aaagcattgg gaatgggcag gggcgagatg aagacttcag gcttcttctt gaggccggtg
     1381 agcggataga tttcgtccac cagacgcacc aaccgtgcat gatcccggtc gttcatgttg
     1441 ggcggacggc agcgcatcat cttgaggacg agattagcgc tgttccacca gccgaacaga
     1501 ggcatcagca cggagaagaa gaagaggatg acggtggcaa cggtgaagtt gacaccaagg
     1561 aagaccagac cggcgaaaga gagaccaccc atcaaggcga tgccaaagat ggtacgaatc
     1621 cagttgatgc gcagttggcg atcgatactg gcctggtcga cagaggggtc tgggccggaa
     1681 tacttgttgg aatgcttatg gtttttcatt gcatgttccc gaagttgtca gacagatgtc
     1741 gttgagtcga agaaaaacgc ctggtccaag caccgctttc cgagaattgc ttctgcgcat
     1801 tgttctcttt ctaagtggcg ctgcagattt gcatttgtgc atgcacatcc gggccgatga
     1861 ctggaaccga gaggttgtcc cgaattctga gccggctccg gaggtagacc tgggagttag
     1921 taattagtga agttggtgcc atccatggtt accagcaagt cgatttgatg acctactccg
     1981 tctcccatgg ttggaagtgg cgatggagaa gagtgactgg cgggattggg aatggaggac
     2041 taaaggaaat tctcttgaga gctgaaaaaa gcgaacttaa ggacactact aaaaatgctc
     2101 ttgaaaaact ctattcaggc ttcagtcgtc cctgcatttt tgatgaaata catcttcggc
     2161 ttgaaaacct tggcgaggca ttagcgct
//
LOCUS       DAOQNZ010000690         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31865_length_2187_cov_4.683396, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000690 DAOQNZ010000000
VERSION     DAOQNZ010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31865_length_2187_cov_4.683396"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PKW73_12185"
     CDS             <1..984
                     /locus_tag="PKW73_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74093.1"
                     /translation="DGSFRNPQVNLRDIEIRRRRSMAGKSKLKNSAEAEVLADHLHDD
                     PQNGLIWLKLCREQAQEGNHRLAIKSIRKALKFSPHLAEAWFLLIDLYKEEENHKQMA
                     NTLRLLQQTRPESVQVWLRMADIFKELNQTAEATECVRRAVQMDPESALCWDAWATRL
                     SEIGDQPGAMDALKHSLSLESVNSQGWTRLGIWRSRCGDFSDARTCFGMACVQDDSNV
                     EAQVWLAKSLDKLGELDMAEKILFGLLEKQPGEARAWNHLGVIFRLRQRMELAEKCFK
                     KALELLPNYPNPWYNLRDIYLQTGEHESAKSAMDSAASISGTRAARFESDG"
     gene            complement(1034..1384)
                     /locus_tag="PKW73_12190"
     CDS             complement(1034..1384)
                     /locus_tag="PKW73_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74094.1"
                     /translation="MSRADDALLYLKDQNVDLVITRVHLDYGNLFSFLKALKDDPQLS
                     ATPVICFCGMLSKCASVQHEFLMNATRIYGATDYLALEHFCKGDDFNLELICQSIERS
                     LTAGNQDMQENSAN"
     gene            1704..>2187
                     /locus_tag="PKW73_12195"
     CDS             1704..>2187
                     /locus_tag="PKW73_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNA74095.1"
                     /translation="MKTTDYSLDELAALVKARLSAVGQLDIGDNRVSPVPDARTTRYY
                     TSIGLLDRPLIVGRQARYTERHLLQLVAIKLLQYLSLPLSTIQSRLYGLSEKELTALI
                     EAIETKLQESRQESAGSDGQFARPVQIWREVVIEPGLKLMVQEDFSTGSPREALLKKI
                     E"
BASE COUNT          542 a          500 c          602 g          543 t
ORIGIN      
        1 gatggaagtt tcagaaatcc acaagtaaat cttcgcgaca tcgagattcg caggagaaga
       61 tccatggctg gaaagagtaa gcttaagaat tcggctgagg cggaggtgct tgctgaccat
      121 ttgcacgacg acccgcagaa tggtctgatt tggctcaagc tttgccggga gcaggctcag
      181 gaaggcaacc atcggctagc aatcaagtca attagaaagg cactaaagtt ttcaccgcat
      241 cttgccgagg cctggtttct tcttattgat ttatacaagg aagaagagaa tcacaaacag
      301 atggcaaaca ccttgaggct tcttcagcaa acccggccgg agagtgttca ggtctggttg
      361 agaatggcgg acatttttaa ggaactcaat caaactgctg aggcaaccga gtgcgtaaga
      421 cgagccgtgc agatggaccc ggagagtgca ttgtgctggg atgcttgggc gaccagattg
      481 tctgagatag gtgatcagcc gggagccatg gatgctctta aacactctct tagcttggaa
      541 tcggtaaact cacaaggctg gaccaggctt ggaatttgga ggagcagatg cggcgatttt
      601 tctgatgcca gaacttgttt cgggatggct tgtgttcagg atgatagtaa cgtagaagcg
      661 caggtctggt tggcaaaaag cctggacaaa cttggtgagc ttgatatggc tgagaaaatc
      721 ctctttgggc ttcttgagaa gcagcccggt gaagctcgag cgtggaatca tttaggtgtc
      781 atctttcgac tgcgtcagcg gatggaactt gcggagaagt gtttcaagaa ggcgctggaa
      841 cttctgccaa actacccaaa cccctggtac aacttgcggg atatctattt acagaccggt
      901 gagcatgagt ctgccaaatc ggcaatggat tccgctgcct ctatttccgg taccagggcg
      961 gctcgattcg agagtgacgg ttgatatatg tattggcgtg agcagcctgg ctgttcggca
     1021 gtgacgcggt aggctagttt gccgagttct cttgcatatc ttggttacca gccgtgagcg
     1081 aacgctcaat gctctggcag attaactcca ggttgaagtc atcgcctttg cagaagtgct
     1141 caagggccag gtagtctgtc gcaccgtata ttcgagtggc gttcatgagg aattcatgct
     1201 gcacggaagc acatttgctg agcatgccgc aaaagcaaat cactggcgtg gcgcttagct
     1261 gtgggtcgtc tttcaaagcc ttcaggaatg agaaaaggtt tccataatca agatgtactc
     1321 tggtaattac aagatctaca ttttggtctt tcaagtataa gagtgcgtca tccgctctac
     1381 tcacaaattt cagctcgtga cgagtttcaa ggcttctttt gagagcggcc ttttctgttg
     1441 gctgttcctg cagaatcaga atttttgcca tttcgggtgc aaacgctacg ccagctcaga
     1501 gattctaaca atccttgaat tctaacagtg ctgcctttca cttgccttac aaccggtgcc
     1561 gggcgttacg ctgccgacta tcgcggctgc aatttaagta tgtggatagg tcgcattcat
     1621 ctgtataggg taggcagacc gggtggttcg aaatgtaaag tgtcaacctt gacagtatta
     1681 cagtaaacgg tgacaatact gtcatgaaga ccaccgatta cagtcttgat gagcttgccg
     1741 ccctggtgaa ggcgcgccta tcggccgtgg ggcaattaga tatcggggac aaccgtgtgt
     1801 cgcccgtgcc tgatgcccgt actacccgct attacaccag tatcggactt ctagaccgcc
     1861 ccctgattgt gggacggcaa gctcgctaca cggagaggca tctgctgcaa cttgtggcta
     1921 taaagctttt gcagtacttg tctctgcctc tttcgacgat tcaatcacgc ctctacgggc
     1981 tctctgaaaa agaacttacg gccttgattg aagccatcga gaccaagctg caagaaagcc
     2041 ggcaagagtc ggcaggaagc gacgggcaat ttgccagacc ggtgcagatc tggcgggaag
     2101 tggttatcga accgggtctg aaactcatgg tgcaggaaga tttctctaca ggaagccccc
     2161 gggaagctct attaaaaaag atcgagc
//
LOCUS       DAOQNZ010000691         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31878_length_2187_cov_3.320356, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000691 DAOQNZ010000000
VERSION     DAOQNZ010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31878_length_2187_cov_3.320356"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKW73_12200"
     CDS             complement(<1..500)
                     /locus_tag="PKW73_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA74096.1"
                     /translation="MKEPANEGNTPDWQSFLAVFQTVVHAYPERPAVIEEGEEFTYRR
                     LYDEALHLAARLKEAGAAPETVLALCLPKSYDYIKALLATWLVRAAFAPVDAALPVQR
                     LQYIFQELIQQNKEQGQNKILVLARDEDQEHLLSAASDQLQLEPVPFTIAHQDELPAL
                     PDTMAQA"
     gene            complement(503..1312)
                     /locus_tag="PKW73_12205"
     CDS             complement(503..1312)
                     /locus_tag="PKW73_12205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA74097.1"
                     /translation="MNAQADTTESKADKPEPETEKNWWLKFYQDTPFIQYLDNAHRED
                     QEEILAFLSVHTPLPEGAVIYDQCCGVGSISLPLAGQGHTVYGTDLCPQFIDEAKRRA
                     EILKVQDRAHYLLADATTYTTPRPCDLAVNLYTSFGYGDDGVNLAMLKRARGCLKEGA
                     PFLLDFPNFYLVISSFRKHIVRHIDTATGTLTVNREAAFSPETGRLLQEWTFIEADGK
                     RSCQKSSLAVYLPHQIKDMLRQVGFERIELMGDFHGTPFGVDGPRCIALAR"
     gene            complement(1309..>2187)
                     /locus_tag="PKW73_12210"
     CDS             complement(1309..>2187)
                     /locus_tag="PKW73_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNA74098.1"
                     /translation="HLSAKHILNKTIHQAGFRVSLTGEGSDEVLAGYPHLRSDLLTGC
                     GEEEKLKSLYAENPMSKGIMLASGASLSLKSVEQTLGFVPSFLKAKGTLGKKMTAVAS
                     PDFLSAFAQRDAYEDLLRHFDVDGQLTGRNRVHQSLYLWSKTALANYILRTLGDGVEM
                     GQSLEGRLPFLDHKLFEFLRHLPLTMKIKGDTEKYILKQALEKHLTRTIYERHKHPFV
                     APPLSRFADQGARRLMQEKLSDPQFARLGFFDQNKLLNLLDNLETLDGEERAAVDPVL
                     MTALSYLSLQRRFMTP"
BASE COUNT          456 a          544 c          636 g          551 t
ORIGIN      
        1 gcctgcgcca tggtatctgg gagcgcgggc agttcatcct ggtgggcgat ggtgaagggc
       61 accggttcca gttgcaattg gtcgcttgct gcactgagaa gatgttcctg gtcttcatcc
      121 cgcgccaata ccagaatttt gttttgcccc tgttctttgt tttgctggat gagttcttgg
      181 aaaatatact gcaggcgttg caccggcaaa gccgcatcca caggcgcaaa agcagcgcgc
      241 accagccagg tggccagcaa ggcctttatg tagtcgtaag acttaggcaa gcaaagggcc
      301 agcaccgttt ccggtgccgc tcccgcttcc ttcagtctgg ccgccaaatg cagggcttca
      361 tcgtagaggc gacgataggt gaattcttca ccttcttcga ttacggccgg tcgctccgga
      421 taagcatgca ccacagtctg aaagactgcc aggaagctct gccaatcagg ggtattgcct
      481 tcgttcgcag gctctttcac gattatctgg ccagtgctat gcagcgagga ccgtcaacgc
      541 cgaagggcgt gccatggaag tctcccatta gttctatcct ttcgaagccc acttgtctga
      601 gcatgtcttt gatctgatgg ggtaagtaga ccgccaggct gctcttctgg caggagcgtt
      661 tgccgtccgc ttcaatgaag gtccattctt gtaagaggcg accggtttcc gggctgaaag
      721 ctgcttctct attgaccgtg agagtgcctg tggcggtatc tatgtggcgc acgatatgct
      781 tgcgaaagct ggaaatcacc aggtagaagt tagggaagtc gagcaagaag ggcgcccctt
      841 ctttcaggca gcctctggcc cttttgagca tggccagatt gacgccgtca tcgccgtagc
      901 cgaaacttgt gtagagattg acagccaggt cgcaggggcg gggtgttgtg taagtggtgg
      961 catcggccaa cagataatga gcccggtctt gcaccttcag tatttcggcg cggcgcttgg
     1021 cctcgtcaat aaactggggg cagaggtcgg taccatatac ggtgtggcct tggccggcca
     1081 ggggcaaact tattgagcca actccgcagc attgatcata gatgacggct ccttcaggta
     1141 agggggtgtg cacagaaaga aatgccagaa tctcctcctg gtcttccctg tgagcattgt
     1201 cgagatactg gatgaatggt gtgtcctggt aaaatttcag ccaccaattc ttttccgttt
     1261 ccggttccgg cttatctgct ttggactcag tcgtatcagc ctgggcgttc atggtgtcat
     1321 aaatctccgt tgcaatgata gataggacag ggcggtcatc aataccggat ccacagcggc
     1381 cctctcttcc ccatcgagag tctccaggtt atccaaaagg ttgagcagtt tgttctggtc
     1441 aaagaagccg agacgtgcaa attgcgggtc ggagagtttt tcctgcatga gacggcgcgc
     1501 tccctggtcg gcgaagcgac tcagaggggg tgctacaaaa ggatgtttgt ggcgttcgta
     1561 gattgtcctg gtcaggtgtt tttccagggc ttgcttgaga atatatttct cggtatctcc
     1621 cttgattttc atagtcaggg gcaggtgtct aagaaattca aagagcttgt gatcgaggaa
     1681 gggcagcctt ccttccaggg attgtcccat ttcgacgcca tcgccgaggg tgcgtagaat
     1741 gtaattggcg agggctgttt tgctccagag ataaagcgat tggtgcaccc gattgcgtcc
     1801 cgtcaattgc ccgtccacgt cgaagtgtcg aagcaggtct tcgtaagcgt ctctttgcgc
     1861 aaaggcggaa agaaagtcag gacttgccac cgctgtcatt tttttgccca gagtgccttt
     1921 ggctttcaga aagctcggca caaaaccaag ggtttgttct acgctcttaa gagaaaggga
     1981 tgcgccggat gccagcatga tacctttgga catgggattt tcggcgtaga gagacttgag
     2041 cttttcttct tctccgcagc cggtcaacaa gtcactgcgc aagtggggat aaccggccag
     2101 cacttcgtcg gaaccctcgc cggtgaggga gacgcggaaa ccggcctggt gaatggtctt
     2161 gttcagtatg tgtttagccg acaggtg
//
LOCUS       DAOQNZ010000692         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31902_length_2186_cov_4.094322, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000692 DAOQNZ010000000
VERSION     DAOQNZ010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31902_length_2186_cov_4.094322"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKW73_12215"
     CDS             <1..883
                     /locus_tag="PKW73_12215"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNA74099.1"
                     /translation="DAEIILAGGMESMSQAPYIVEANSARFGKRMGHVAFQDAMLVDG
                     LECPVTGVHMAVHGAKVAEEFSIGREEQDEWAVRSHKKAVEAQEKGAFKKEILAVEVP
                     AGKGQTKLVDKDESPRADTSKEALAKLKPVFYDKGSITAGNAPGVNDGACMLLVMSAG
                     KAKELGLKPLAKIVSHASIGQDVPYLATVPALATEKALKKAGLKVEDLDLMEINEAFA
                     AVALKSIKMLGIDPEKVNVNGGAIALGHPIGASGARILLTLAREMESRGAKYGAAAIC
                     SGTAQGDCVILSREGLD"
     gene            1034..>2186
                     /gene="ligA"
                     /locus_tag="PKW73_12220"
     CDS             1034..>2186
                     /gene="ligA"
                     /locus_tag="PKW73_12220"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNA74100.1"
                     /translation="MVEGQVSVQDAKARIDELSRLVEHHRFCYYVGEPEIIDAEFDKL
                     FYELQELEKAYPDLARPDSPTQKVGAKPSTDFKEVVHRIPLLSLANAMGEDDLMHWQE
                     RLARGLKEAEREESVLSFVCEHKIDGLSVALSYKDGHFFQGATRGNGEVGEDVSLNLK
                     TIAGLPRQIKPVAVMKDGTVRAPSADLPAGSYELKVPAQVEVRGEVYMPVSSFEALNA
                     QLLEDGQEPFANPRNAASGSLRQKDPSKTAKRKLALWAYFLYILDEGLKQPDSHFENL
                     ALLEAMGFPVEPNRTLTGAEGLMQFVQNWHMPRHKLDYQTDGVVIKLDQRPLWNLLGN
                     TSHSPRWAIAYKYPPEEAETVIEEIHFDVGRTGAVTPVAWLKPVKLAGTT"
BASE COUNT          506 a          577 c          626 g          477 t
ORIGIN      
        1 tgatgccgag attattctgg ccggcggcat ggaatcaatg agccaggctc cttacattgt
       61 cgaagccaac tccgctcgct tcggtaagcg catgggtcat gtggctttcc aggatgccat
      121 gcttgtagat ggtcttgaat gccccgtcac cggcgtgcac atggctgtac atggagccaa
      181 ggtggcggaa gagttttcta tcggccgcga agagcaagac gagtgggccg taagaagcca
      241 taaaaaggct gtggaagcgc aggagaaagg tgctttcaag aaagaaattc tggcggtgga
      301 ggtgccggcc ggcaaaggtc agaccaagtt agtcgataag gatgaatcac cccgcgccga
      361 taccagcaag gaagctctgg ccaaactgaa gccggtcttc tacgacaagg gcagcatcac
      421 cgccggcaat gccccgggag tcaatgacgg tgcttgcatg ctcctggtta tgagtgccgg
      481 caaagccaaa gagcttggtc tcaagcccct ggctaagatt gtttcccatg cttccatcgg
      541 tcaagatgtt ccttatctgg ccacagtgcc tgccctggct actgagaaag ccttgaagaa
      601 agccggactg aaagtcgaag atctggatct catggaaatc aacgaagctt tcgccgccgt
      661 tgcgctcaag tcaatcaaaa tgctcggtat cgatcccgag aaggtcaatg tcaatggtgg
      721 tgccattgcc cttggtcatc ccattggtgc aagcggcgct cgtattctcc tcacattggc
      781 tcgcgagatg gaaagccgtg gcgctaaata tggtgcagct gccatctgtt ccggcaccgc
      841 ccagggtgac tgtgtcatct tgagccgtga gggtcttgac taaattagtc aagttggcaa
      901 actgacaggc agggcggcgc cgctttcctt tttcaaaagt attctttatg aaaagatgga
      961 acggcgccgc ttccctttta cagatgagat ggtctctggc gtgtggtcct taggagagaa
     1021 gcgggagaga gctatggttg aaggtcaggt ttcggtacag gatgctaagg ctcgcataga
     1081 tgagctcagt cgtttggtgg agcaccaccg cttctgctat tacgtgggtg agccggagat
     1141 tatcgatgcc gagttcgaca agctctttta tgaactgcaa gagctggaga aggcctatcc
     1201 cgacctggcc agaccggaca gccccactca gaaagtaggg gccaagccca gcaccgactt
     1261 caaggaagtg gtgcaccgta ttccacttct cagcctggcc aatgccatgg gtgaagatga
     1321 cctgatgcac tggcaggagc gcctggctcg tggtttaaaa gaggctgagc gggaagagtc
     1381 ggtgctcagt tttgtctgcg aacataagat agacggactc tccgtcgcgc tctcctacaa
     1441 agatggtcac ttcttccagg gtgccaccag gggtaatggc gaagtcggtg aagatgtcag
     1501 cctcaatctt aagacaatcg ccggtttgcc acgacaaatc aagccggtgg ccgtcatgaa
     1561 agatggcacc gtgcgcgccc cctcggccga tcttccagcc ggcagctatg agctgaaagt
     1621 accggcgcag gtggaagtgc gcggcgaagt ctatatgccg gtgtccagct tcgaagctct
     1681 caatgcccag cttctggagg atgggcagga acctttcgcc aatccccgta atgccgcttc
     1741 cggctctctg cgccagaaag atccaagtaa gacagcaaag agaaaactgg ctctctgggc
     1801 ttattttctc tacattctcg acgaaggtct caagcagccg gatagccatt ttgaaaatct
     1861 ggctctgctt gaagccatgg gttttcctgt ggaacccaat cgtactttga ccggtgccga
     1921 ggggctcatg cagttcgttc agaactggca tatgcccagg cacaagctcg attaccagac
     1981 cgacggcgtg gtcataaagc tcgaccagcg tcccctctgg aatcttctcg gcaataccag
     2041 ccattctcct cgctgggcga ttgcctacaa atatccaccc gaggaagccg agacggtcat
     2101 tgaagagatt catttcgatg tgggccgcac cggcgccgtc actcctgtgg cctggctcaa
     2161 accggtgaaa ctggccggca ccacgg
//
LOCUS       DAOQNZ010000693         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31929_length_2185_cov_3.746948, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000693 DAOQNZ010000000
VERSION     DAOQNZ010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31929_length_2185_cov_3.746948"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PKW73_12225"
     CDS             complement(<1..918)
                     /locus_tag="PKW73_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNA74101.1"
                     /translation="MARAEVLCIGTELLLGDVLDTNSQFLARELALLGIDCFYRVVVG
                     DNPERIKECVRQALGRADIVITSGGLGPTADDLTTECLAGVFNAPMDMDEEVLAFIRE
                     LFASRNIKMTESNRKQALRPRGADILPNPVGSAPGIVWQLQKADLDGVGIDSSYVSAF
                     GRDNPYRVILTFPGVPSELKAMWTETAAPYLARYYPAGTIWSTELKHIGIGESALAEK
                     YAHLLDLPNPTVAPYAGRWECRLRVAAKAASQEEARLLAQPVVDEIRQGSGVTLYGED
                     DDTLEAVVGRLLTGHGKTLSLAESCTGGLI"
     gene            complement(927..1082)
                     /locus_tag="PKW73_12230"
     CDS             complement(927..1082)
                     /locus_tag="PKW73_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74102.1"
                     /translation="MSDATHKDPAKSWMVFFFVCFASSVLAIGVWAAFNYKVDAPEPV
                     AAAGGHH"
     gene            complement(1195..1803)
                     /locus_tag="PKW73_12235"
     CDS             complement(1195..1803)
                     /locus_tag="PKW73_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74103.1"
                     /translation="MRLPFAIKSLAGLGAMLLFLGGFLGVQAVDYLGRLGKRMVDPVA
                     LKSIASELGAGAVGLNPYLGLKDRGGLVCFLEEARAEKQVLFLRLDLEDPKEYFDENR
                     FLEQACENVLVGSGSQPQFARLSSVKERDKQELADGSGKLILRFQLLTVKGLADKQYA
                     GLVACLRRGRNFYLAQVSQPADRSYDKDAALGLVKGLFGLPN"
     gene            complement(1807..>2185)
                     /locus_tag="PKW73_12240"
     CDS             complement(1807..>2185)
                     /locus_tag="PKW73_12240"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843924.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HNA74104.1"
                     /translation="GRSALVGKPVALLLLGKDATVTLCHSRTKDLDAVCRSADVLVAA
                     CGRANMIKGDWVKPGAVVIDVGINRVEDANGKGRLTGDVDYEPAAARASAITPVPGGV
                     GPMTVAMLLSNTFVSWKRRLGVK"
BASE COUNT          483 a          680 c          605 g          417 t
ORIGIN      
        1 tataagcccg ccggtacagc tctcggccag ggaaagggtc ttaccatgac cggtgagaag
       61 gcgccccacc accgcttcca gggtgtcgtc gtcttcccca tagagagtta ctccgctgcc
      121 ctgtctgatt tcatccacca cgggctgagc cagcaggcgc gcttcttcct ggcttgcggc
      181 tttagcggca acccgcaggc gacattccca gcgaccggca taaggagcca cggtgggatt
      241 gggcaagtcg agcaaatggg cgtatttctc tgccagggcc gactctccga taccgatgtg
      301 tttgagttcg gtggaccaga tggtgccggc tggataatag cgagccagat aaggagcggc
      361 cgtctccgtc cacatggcct tcagctcgct gggcacgccg gggaaggtga ggatgacccg
      421 gtagggattg tcccgaccaa aggcggaaac gtaacttgag tcgatgccga cgccgtcgag
      481 atcggctttt tgcaactgcc agactatgcc cggcgccgag cccaccggat taggcaggat
      541 atcggcaccg cgagggcgca gggcctgctt gcgattgctc tcggtcattt ttatgttgcg
      601 actggcgaag agctctcgaa taaaagcaag gacctcttca tccatgtcca tgggggcgtt
      661 gaaaacaccg gccagacatt cggtggtcag gtcgtcggcg gtagggccga gccctccgga
      721 agtgatgaca atatcggccc gtcccagggc ctgccggacg cactctttga tgcgctcggg
      781 attgtctcct accaccacgc ggtaaaagca gtctatgcca agcaaggcca gttcccgagc
      841 caggaactga gaatttgtat caaggacatc gcccagaagg agttcggtgc cgatacatag
      901 gacttcggct ctagccacgg ccgactctaa tgatggcccc cggcagcggc caccggctcc
      961 ggagcgtcta ccttgtagtt aaaagcagcc cagactccga tggcgagcac ggaagaagca
     1021 aaacaaacga agaagaatac catccaagat ttggccgggt ctttatgagt cgcatcgctc
     1081 atttactgca gtctcctcaa aatcgattta taactagcgc tgacccaagt ataagccctt
     1141 ggctgccccc aagaaaacaa actcgacctc aaagaaaact aatggcccgc cccttcagtt
     1201 cggcaggccg aacagcccct tgacgagccc cagggccgca tccttatcat aactgcggtc
     1261 ggccggctgg ctcacctggg ccaggtagaa attgcgcccc cgcctcaggc aagccaccag
     1321 cccggcatac tgcttgtcgg caaggccttt caccgtcaaa agctgaaaac gcagaatcag
     1381 tttgccgctg ccatctgcca gttcttgctt gtctctttcc ttcacggatg agagcctggc
     1441 gaactgaggc tgactgccgc ttccgaccag tacgttttca caggcctgct ccaggaagcg
     1501 gttttcgtca aagtactcct tgggatcttc cagatccaga cgcaaaaaga gaacctgctt
     1561 ttcagccctg gcttcctcga gaaagcagac caacccgccc cggtctttca gccccaggta
     1621 gggattgaga cccaccgccc ccgcacccag ttcggaagcg atgctcttga gggcgaccgg
     1681 atcaaccatg cgtttaccca gccgccccag atagtccacc gcctgcacgc ccagaaagcc
     1741 tcccaggaaa agaagcatgg ctcccagtcc ggccagggat ttgatggcaa agggcagtcg
     1801 cacgctctat ttaactccca gacgccgctt ccatgagaca aaagtgttgg agagcaacat
     1861 ggctaccgtc atgggaccaa cgccgccggg aacgggcgta atagctgaag cacgggcggc
     1921 cgccggttca taatccacat caccggtcag tctccctttg ccgttggcgt cctccacccg
     1981 gttgataccc acgtctatca ctacggcgcc gggcttcacc cagtcaccct tgatcatatt
     2041 ggcccggcca caggcagcca cgagcacgtc ggcgctgcgg cagacagcgt ccagatcttt
     2101 ggtacgggaa tggcagaggg taacagtggc atctttgccc agaagtagta acgccaccgg
     2161 cttaccgacc agggcggaac ggcct
//
LOCUS       DAOQNZ010000694         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_31974_length_2183_cov_3.926222, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000694 DAOQNZ010000000
VERSION     DAOQNZ010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31974_length_2183_cov_3.926222"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PKW73_12245"
     CDS             complement(<1..297)
                     /locus_tag="PKW73_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74105.1"
                     /translation="MLASDSASRERLELETRVLAAALRQSLELGDMEGSTLAHEQIFI
                     LLRACQDMQGLQSFEGAAKEGGGDLSVVRGRRIPDIQWFSDDKSGVSSGKAEPGS"
     gene            540..1313
                     /gene="rsmG"
                     /locus_tag="PKW73_12250"
     CDS             540..1313
                     /gene="rsmG"
                     /locus_tag="PKW73_12250"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNA74106.1"
                     /translation="MKSNSESTENRPTELCALTEKVAKQAELAQLPIEAQTCQKIADF
                     VDFLWDYNRRLNLVGTKDIEQIIEKHVLDAFTVAKTFQKQAEAKSYVDLGSGGGLPGM
                     LVALVNPALEVTLVDAIGKKCRFLEEATDKLCRGYRAPVRIVCARAEELFRQPDCREH
                     FDLGTARAVGSLKLTMELVAPALKAGGFYLAQKTLVREEEERQEAALLQEKLSLKLVD
                     RQTFETLPSIRENVLLTFLKEKPTSALYPREWKKIKGDN"
     gene            complement(1339..>2183)
                     /locus_tag="PKW73_12255"
     CDS             complement(1339..>2183)
                     /locus_tag="PKW73_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74107.1"
                     /translation="NPYARCEQSIKVLEKLFEENLCRADSKHLEVAAVCAYWEEFFHL
                     EDPLHDEKEWKEGLSKRSGQYDNFEKWQEWFKSSYAKVYTENNIWTSLLMTSWATTHQ
                     YQLIYTEDSWDFVPSRSYLPTSHMWRLSHHIDTVTAVLKEFWAEESKRWEDAGFIIED
                     ESTHYLGRVECLGFDTKRYMVTWPARAHINTSGGGGAHREDLDLGNLVADIITPVGDN
                     WYRRPDSVIFAHDHVDITSLINRATYVFGSKRRIFELALDDDMNKAKLQAQAEGWLVE
                     DLVV"
BASE COUNT          558 a          581 c          565 g          479 t
ORIGIN      
        1 ggagcccggc tcagctttgc cgctggatac gccactcttg tcgtcggaaa accactgaat
       61 gtcaggaatg cggcgccctc tgacaacgct caaatcgcca ccgccctcct tggcggcacc
      121 ctcaaagctt tgcagaccct gcatatcctg acaggctcga agcagaataa aaatctgctc
      181 gtgcgccagg gttgagcctt ccatgtcgcc aagttccaga ctctggcgca atgcagccgc
      241 cagaacacgc gtttccagct ccaatctttc tctggaagct gagtctgaag ctaacacttt
      301 gaaaacccct gcaaactagc aaatggtgac ggaaaaacga agttttccaa tgtatccaac
      361 aaaattttcg gctggtaagt taataagttt ggtccatttt gaaattgaaa caattaggtc
      421 tttcaaattc ccccattttg gcactatcct aaacaaatcg cccgccggca tgagcgtaca
      481 tcatactttc ttccctaatt cacagccatt ctcacggtca gaccgagagg aaataccaga
      541 tgaaatcgaa ttctgagtct acggaaaacc gcccaacaga gctttgcgcc ctcactgaaa
      601 aagtggcgaa acaggcagag ctggcccaac tgcccataga agcccaaacc tgccaaaaaa
      661 ttgccgactt cgtggacttt ctctgggact acaatcgacg tttaaatctg gtgggaacca
      721 aggacatcga gcaaataatc gagaagcatg tgctggatgc tttcacagtg gcaaaaacct
      781 ttcaaaaaca ggcagaagcc aaaagctacg tggatctggg cagcggcggc ggtctgcccg
      841 gtatgctggt agcgcttgtc aatcccgccc tggaagtgac cctggtggat gccatcggca
      901 aaaaatgccg cttcctggag gaggcgactg ataaattgtg cagagggtac agagcgccgg
      961 tgagaatagt ctgcgccaga gcggaagagt tgttcagaca gcccgactgc agggaacatt
     1021 tcgatctcgg cacggcaaga gcggtgggaa gcctcaaact gaccatggaa ctggtggcgc
     1081 ccgccctcaa agcggggggc ttctacctgg cccagaagac ccttgtgagg gaagaggaag
     1141 agcggcagga ggcggcactc ttacaagaga aactgtcact caagctggtt gacagacaaa
     1201 cttttgagac gctgccctcc ataagagaga acgtgctcct taccttcctg aaagagaagc
     1261 ccaccagcgc actgtatccg cgggaatgga agaagataaa gggggacaac tgagctttat
     1321 cagcctgcag tatcgctatc agacgaccag atcttccacc agccagcctt ccgcctgcgc
     1381 ctgcaatttg gctttgttca tgtcgtcatc aagagccagt tcaaaaattc gtctcttgct
     1441 gccgaaaaca taagtagccc ggttgatcag gctggtgata tccacgtggt catgggcaaa
     1501 gattaccgag tcggggcgtc tgtaccagtt atcgcccacc ggcgtgatga tatccgccac
     1561 cagattgccc agatcgagat cttcgcgatg agcaccgcca ccgccggaag tgttgatatg
     1621 ggccctggca ggccaggtga ccatatagcg cttggtgtca aagcccagac actccacacg
     1681 gcctagataa tgcgtgctct cgtcttcaat gatgaagccg gcgtcttccc agcgctttga
     1741 ttcttccgcc cagaactcct tcagaacggc ggtgaccgtg tcgatatgat ggctgaggcg
     1801 ccacatgtga gaagtaggca aataggaacg cgagggcacg aaatcccagg agtcttcggt
     1861 gtagatgagc tggtactggt gggtggtggc ccatgaggtc atgagcaggg aggtccagat
     1921 gttattttct gtatatactt tggcgtagct ggacttgaac cattcctgcc atttttcaaa
     1981 attgtcgtac tgaccggagc gtttagagag cccttccttc cattcttttt cgtcgtgaag
     2041 agggtcttcc agatggaaga attcctccca ataggcacaa accgccgcca cttccaaatg
     2101 cttgctgtcg gcgcggcaaa gattttcttc gaaaagcttt tccagcactt tgatggactg
     2161 ctcacaacga gcatagggat tgg
//
LOCUS       DAOQNZ010000695         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32013_length_2182_cov_3.422661, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000695 DAOQNZ010000000
VERSION     DAOQNZ010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32013_length_2182_cov_3.422661"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="PKW73_12260"
     CDS             complement(<1..1541)
                     /locus_tag="PKW73_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74108.1"
                     /translation="MLYIFVFLGGFTSLVYQVIWQRLSAFSLGSDASAAAVVAAAFMT
                     GLGLGSLGGALTCSRLRRRAAVLVLFLIELGIALAGVLSLPVLYRCFYLELSDPQQMQ
                     NLAPTIFLLLTLPTILMGLSFPILTRLAVSALEGSAVKISTLYGFNTLGAALGAIIGG
                     LILMRHLGLETTIFMTAALNLLAALIILTSAKEGSAQPMPEISENPDATDKKDLSRSD
                     ATGRFYAWCSLYALSGFVNLGLEIVWFRLLGVMLKSNTFTYSILLFFYLAGLGAGSIF
                     AARCLKQASSKTFLVLQLALTAYTAFFLVLIYREAQPEGLLNWLHTYLGNYNPLELAD
                     YSLSAIFSENGELFRSLYFFLAAFMILPPTFMMGVSFYGLQNSVQNDLKQIGRRVGLL
                     QMFNIAGGTLALLWLGIWGLKHIGTFSVLNILMLSNQIFLPIYFLLKYTGEKEKPGAA
                     AECIKKGLVYLTISLLPCLAIPSAEKMWIALHGGSTYFDFEEDHTGIVAIKHAYEDHK
                     FIFING"
BASE COUNT          602 a          510 c          544 g          526 t
ORIGIN      
        1 ccgtttataa aaataaactt gtggtcttca tatgcatgct tgatggctac tattcccgtg
       61 tgatcttcct cgaagtcgaa gtaggtactg cctccatgca gagctatcca cattttttcc
      121 gcagaaggaa tagccaggca tggtagaagc gagatggtca ggtagacaag ccccttcttt
      181 atacattcgg cagcggcacc gggtttttct ttctcgccgg tgtatttgag tagaaaatag
      241 attggcagga aaatctgatt gctgagcatc agaatattca acacgctgaa tgtaccaata
      301 tgcttgagcc cccatatacc aagccagagc agtgccaggg tgccacccgc tatattgaac
      361 atctgcaaga gtcctactcg cctgccgatc tgtttcagat cgttttgtac cgagttctgc
      421 agtccgtaaa aggaaactcc catcatgaag gtggggggca aaatcatgaa ggccgccagg
      481 aagaagtaga gggagcggaa gagttctcca ttttcggaaa aaattgcgga caaactgtag
      541 tcggctagtt ccagcggatt atagttgcct agataagtat gcagccagtt gagcaagcct
      601 tcaggctgcg cttctcgata aatgagtacc agaaaaaacg ccgtgtaggc tgtaagcgcc
      661 agttgcaaaa ctagaaatgt cttgctggaa gcctgcttca aacaacgagc ggcaaaaatt
      721 gatccggctc ctaagccggc caggtagaag aagagcaaaa tactgtaggt aaatgtattt
      781 gacttgagca ttactcccag taagcggaac cagacaattt ccagacccag attgacgaag
      841 ccggagaggg cataaaggct gcaccaggca tagaatcttc ccgttgcgtc cgagcggctc
      901 aggtcttttt tgtcagtggc gtccgggttt tccgagattt ccggcattgg ttgtgcactg
      961 ccttcctttg cgctggtcaa aatgatcagt gcggccagca gattcagcgc cgcagtcatg
     1021 aagattgtgg tttcaagacc cagatgacgc atcaggatta agccgccgat aatggctccc
     1081 agcgcagcac ccagggtatt gaatccataa agagtggaaa ttttgacagc cgaaccttct
     1141 aaagctgaga cggccagtcg ggtcagtatc ggaaaactga gccccatcaa aattgtaggt
     1201 agggtcaaga gcagaaaaat tgtcggcgcc agattttgca tctgttgagg gtctgacagt
     1261 tcaagataga agcaccggta gagtacaggc agacttaaaa caccggctaa agcaatgccc
     1321 agttcaatca aaaagagcac cagtactgct gcccttcgcc gcagacgtga gcaggtcagt
     1381 gctcccccga ggctgccaag accaagcccg gtcataaagg cagctgctac cacggcagct
     1441 gcactggcat ctgagccgag ggagaaggcc gacagtctct gccaaattac ttgatataca
     1501 agagatgtga atccgcccag gaatacaaag atgtagagca agattggtct gatccgatat
     1561 aggtgttatc gcccatacaa agtataggca ttactctgga gaaattctcc accaaatcga
     1621 gagaacggac gacatcttga aagcctgatt gcaagggcat ttccgttaat gctgagaagc
     1681 tattaataag ttccaatagc atatacgatc gtcgctttgc ccatggcacg ggcatgaaaa
     1741 gtggcttgta ttgctgattt taatgccctc atgtagtagg tgtccggaag tcctgaactg
     1801 atgcgtgtct tgaaaaattt atcttcactc atctgcgaag gctgatagtg ggcgttttca
     1861 ggaggttcac acctcttgag tttcgaggat tgtatatagc taatctatct aaagtgtagt
     1921 cgtcatgctg tattgcaaag ttggttagac cgggcttatg ataactgaca tagaaacgca
     1981 attgaattcc aaagacatcg gcaaacgagt ttccactact tgctttgtac tgacggtgtt
     2041 actggactcc tggtgaaaga ctcaagtcac tcaccggatg gcacacctgc ttctcttgtt
     2101 gttctctacc aataaaacca aaaaccacaa gccccaaagt gcactttgag ccggccctta
     2161 ctctgtctgc cggcgcggtg cc
//
LOCUS       DAOQNZ010000696         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32023_length_2182_cov_2.808181, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000696 DAOQNZ010000000
VERSION     DAOQNZ010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32023_length_2182_cov_2.808181"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKW73_12265"
     CDS             <1..297
                     /locus_tag="PKW73_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74109.1"
                     /translation="INNLLDIARMESGSLQLHYAEVTAAELIAPAFEQVTLLAEDRQL
                     RLVSQVAADVPAFRVDVDLLSRVLVNLLGNAIKYSPADTTVYLGQRHTGHPRIG"
     gene            290..550
                     /locus_tag="PKW73_12270"
     CDS             290..550
                     /locus_tag="PKW73_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA74110.1"
                     /translation="MVEFSVADQGVGIPEEHLPQVFSKFGRLDNPLVRQTEGTGLGLY
                     ITRSLVLALGGQITVESVPGGTTFTVRLPAATPEQQAARGRG"
     gene            552..1922
                     /locus_tag="PKW73_12275"
     CDS             552..1922
                     /locus_tag="PKW73_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA74111.1"
                     /translation="MLLPTPVILIINKPRQAAEFAQVLEKTGAKVHAATSFEEANELL
                     SFLHPDIIILPNKLDGDEGGDGRLYCQQVRATLAPPRPVLVLLHPSTDVTERINAFRY
                     GADDVLGDPIDKNELAIRVLAHLRRRQEEFSHQLTQLPGPNLIRRMLDQCLYSDRHWA
                     ALSIDLNKIRVYNETYGDLAGDQLIKALAAILTDIADETEFVGHIEADDFLMVTRPEL
                     AEKRAELICRQFDSISSRFYPKGDIERGYLISTGRGGIRRRVPLISIAVGIVSSTVRK
                     FQSYVDVLTTARDYRYLAKQKQGSAWISDKNIDECRTMMLQTLALEGESKEAEVPERT
                     TRILVVEPDGAMACLLQETLALEGYYVQVTSSADDALELARYQPHPDLILLESNLTGR
                     KLDGWKLCRALKSDEELSKIWIVMATSHPDQSLALEAGADLYLPKPFDMPSLLSEVSL
                     LLRSRF"
     gene            complement(1911..>2182)
                     /locus_tag="PKW73_12280"
     CDS             complement(1911..>2182)
                     /locus_tag="PKW73_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNA74112.1"
                     /translation="GLVEQAFREHPDLDRQLSFVVGDKATDVELARNCAARGVLVETG
                     YGKEVLAGTYQWKVEPDFQAGSIVEAANWIIEQVKSRVAKSSSEA"
BASE COUNT          512 a          665 c          548 g          457 t
ORIGIN      
        1 atcaataatt tgctggacat tgcccgcatg gagagcggct cgctgcagct gcactatgcc
       61 gaggtcaccg ctgccgagct gatcgcgccc gcctttgagc aggtaacgct gctggccgaa
      121 gaccggcaat tgcggctggt gtcgcaagtc gcggccgacg tgcccgcctt tcgcgtcgat
      181 gtggacctgt tgagccgcgt gctggttaac ttactgggca atgccatcaa gtattcaccg
      241 gccgacacca ccgtctatct cggccaaagg catacagggc acccccgaat tggttgaatt
      301 ctcggtcgca gatcagggcg tgggcatacc ggaagaacat ctgcctcagg tcttttccaa
      361 attcggcaga ctggataatc ccctggtcag acagaccgaa ggcaccggac tggggctcta
      421 catcacccgc tccctggtac tggctctggg cggtcaaatc actgtagaaa gcgtaccggg
      481 cggcaccact ttcacagtgc gtctgccggc agcgaccccg gaacaacaag cagcaagagg
      541 taggggttaa gatgctgcta ccgacaccag taattctcat catcaataaa cccaggcagg
      601 ctgcggaatt cgcgcaggta ttggagaaga ccggtgccaa ggtacatgcc gccacatcct
      661 ttgaagaggc caacgaatta ctctccttcc tgcacccgga catcatcatc ttgcccaata
      721 aattagacgg tgatgaaggt ggagacgggc gtctctattg ccaacaagta cgggccacac
      781 tggccccacc aaggccggtg ctggtgctcc tgcacccctc caccgacgtg accgagcgca
      841 tcaatgcttt ccgctacggt gccgacgatg ttctgggcga tcccatcgat aaaaacgagc
      901 tggccataag ggtgctggcc cacctgagaa gaagacagga agagttctcc caccagctta
      961 cccagcttcc cggtcccaat ttaattagac gcatgcttga tcaatgtctc tattcggata
     1021 gacattgggc ggctctttct atcgacctca ataaaattcg tgtctacaat gaaacctatg
     1081 gagacctggc aggcgaccaa ctgatcaaag ccctggccgc cattctcacc gatatcgccg
     1141 acgaaacgga atttgtggga catatcgagg ccgacgactt ccttatggtg accagaccgg
     1201 agctggcaga aaaaagagcg gaactcatct gtcggcaatt tgacagcatc agctcacgtt
     1261 tctatccaaa gggagacatc gaacgcggtt atctgatctc caccggccgt ggtggcatac
     1321 ggcggcgcgt gcccctcatt tccatcgccg ttggtatcgt atccagtacc gtcaggaaat
     1381 ttcaatccta cgtcgatgtg ctcaccaccg cccgcgacta ccgttatctt gccaagcaaa
     1441 aacaaggcag tgcctggatt tccgacaaaa acatcgatga gtgccgcact atgatgctgc
     1501 aaactctagc cctggaaggc gaaagcaagg aagccgaagt accggagcgc accactagaa
     1561 ttctggtagt agaaccggac ggcgccatgg cttgtttatt gcaggaaact ctcgccctgg
     1621 aaggttatta cgtgcaagtg accagttccg ccgacgatgc cctggaactg gctcgctatc
     1681 agccccaccc cgatcttatt ttgctcgaat ccaacctgac cggcaggaaa ctagacggtt
     1741 ggaagctctg ccgtgctctt aagtcagacg aagagctatc caagatctgg atcgtcatgg
     1801 ccacctccca tcccgatcaa tcactggccc tggaagcggg cgccgacctc tacctgccca
     1861 agcccttcga catgccgtcc ctgctctcgg aagtcagcct cttactgcgc tcacgcttct
     1921 gacgaacttt ttgcaactcg gcttttgacc tgctcaataa tccagttggc ggcctccact
     1981 atagaaccgg cctgaaaatc aggctctacc ttccactgat aggtaccggc cagaacttcc
     2041 ttgccatagc cggtctccac cagaacgccc ctggccgcgc agtttctagc taactccaca
     2101 tcggttgcct tatctcccac tacaaacgat aactgacggt ctaaatccgg atgctcacgg
     2161 aaggcctgct ctacaagacc cg
//
LOCUS       DAOQNZ010000697         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32153_length_2177_cov_3.694156, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000697 DAOQNZ010000000
VERSION     DAOQNZ010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32153_length_2177_cov_3.694156"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PKW73_12285"
     CDS             <1..207
                     /locus_tag="PKW73_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74113.1"
                     /translation="TLPWLDRGPTPSLPELPFSIALGHPLGASGARILTTLVHEMQRR
                     DSKTGLATMCIGVGQGIATIIARD"
     gene            223..1554
                     /locus_tag="PKW73_12290"
     CDS             223..1554
                     /locus_tag="PKW73_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74114.1"
                     /translation="MTAAERTTFKFLQFSDVLLDSKLTIGSVSAQPGQLNCGYSLPRT
                     ERQERAREILETTINAFSLAASENVDAVLIPGGLFEAATVSGYTINTLIESIATIEHI
                     PVFIAPGVADYYTRSSLYAADMLQARGLKTWPKNVHIFSSEHFSAQPLPNKPQVNIIG
                     RAITKGVRRTERVLSGKLPKPAGSQINVALLVGSLESHPQIEGRVDSKSILYPFSQQE
                     MESMGMTYFALGHADSAFKVESESGTTLGAYAGCLAAGTFDKPGARYALLGEIEVDEA
                     GTVNCDLNPVEIDERRMMVVTVDVSGLGDADIKEEIMVAVEYAEVRPEVDVVALSLEG
                     GCKPGANPRKVADSLAPQFHCLLVVDNTRPDYLAERFDERTTEWKYIQAMLEMKTKAE
                     RTLNRMQSEHSSSDSLSGIPGTDLSGKTVEDALYYGLDALRQKRVTIRNVG"
     gene            1544..>2177
                     /locus_tag="PKW73_12295"
     CDS             1544..>2177
                     /locus_tag="PKW73_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA74115.1"
                     /translation="MWVEKVELTSYGGIQGESVIFSQDKVNLVVEPNEYGKTTMATAI
                     WSILFDFPSEEQDAADRLTSKDARRPKPGQPYQAKMDVNTDSRRLTVKRDFQTGAFQV
                     LDRDHLDKDVTAEFLGQNGEDEVGLKLTGMTRELFKSTCFVGQRELDEHAFGGDDMAS
                     LVQGIADRATLAAASLIDASNDEITKAVNGFIADAKTAEKVGSIDKVTVSF"
BASE COUNT          553 a          662 c          582 g          380 t
ORIGIN      
        1 accctgccct ggctggaccg ggggccgacg ccgagcctcc cggagctgcc cttctccatc
       61 gccctgggcc acccgcttgg cgccagcgga gcgagaattc tcaccaccct ggtgcacgaa
      121 atgcaacgcc gcgactctaa aacaggtctg gccacaatgt gtatcggcgt cggtcagggc
      181 atcgccacca tcatagctag agactaagag aggttctata aaatgacagc cgccgaaaga
      241 accactttta aattcctgca attctccgat gtgcttctcg acagcaagct caccatcgga
      301 agcgtctctg ctcaaccagg ccagctcaat tgcggctatt cactgccacg cacggaaagg
      361 caagaaagag ccagggaaat tctcgagacc actatcaatg ccttttccct ggcggccagc
      421 gaaaacgtcg acgccgtgct tattccgggc gggctcttcg aggcagccac ggtgagcggc
      481 tacaccatca acaccttgat tgaaagcatt gccaccatcg aacacattcc cgttttcatt
      541 gccccgggag tggccgacta ttacacgaga agctcgctct atgccgccga tatgctgcag
      601 gcgcgcggac tcaagacctg gcccaagaat gtgcacatct tctcttccga acatttttcc
      661 gcccaacccc tacccaacaa accccaggtc aacatcatcg gcagggccat cactaaaggg
      721 gtgcggcgca ccgaacgggt actctcaggc aaactgccca agccggccgg cagtcaaata
      781 aatgtagcgc tcctggtggg ttccctcgaa agtcatcctc agatagaagg cagagtagac
      841 agcaagagca ttctctatcc cttctcccaa caagaaatgg aaagcatggg catgacctac
      901 tttgccctcg gacatgccga cagcgccttc aaagtagaat cggaaagcgg caccaccctg
      961 ggtgcctatg ccggttgcct ggcagccgga accttcgaca aacccggcgc ccgttatgcc
     1021 ctgcttggcg aaattgaagt agacgaagcc ggcaccgtca actgcgactt gaaccccgta
     1081 gagatagacg agcggcgcat gatggtggtc accgtcgatg tctccgggct cggagacgcc
     1141 gacatcaaag aagaaatcat ggtagccgta gaatacgcgg aagtgagacc ggaagtggat
     1201 gttgtagcct tgagcctgga agggggctgc aagcccggtg ccaaccccag gaaagtggct
     1261 gacagcctgg cgccgcaatt tcactgcctg ctggtggtgg acaacactcg ccccgactat
     1321 ctagccgagc gcttcgacga gcgcaccacc gaatggaagt acattcaagc catgctagaa
     1381 atgaagacca aggctgaaag aacattgaac cgcatgcagt cggaacattc cagttccgac
     1441 agtctctccg gtataccggg caccgatctt tccggcaaaa ctgtggaaga tgccctctac
     1501 tacggactgg atgctctcag acaaaaacgg gtgacaataa gaaatgtggg ttgagaaagt
     1561 cgagttaacc agctacggcg gcattcaggg cgaaagcgtc atcttcagcc aggacaaggt
     1621 aaacctggtg gtggagccca acgaatacgg caaaaccaca atggccacgg ccatctggtc
     1681 catcctcttt gacttcccca gcgaagagca agacgcagcc gaccggctca ccagcaagga
     1741 tgcacgccgt cccaaacccg gccagcccta tcaagccaag atggacgtca acaccgacag
     1801 tcgcaggctc accgtcaaaa gagacttcca gaccggcgcc ttccaggtgc tcgaccgaga
     1861 tcatctagat aaagacgtga ccgccgaatt tcttggtcaa aacggcgaag acgaagtcgg
     1921 tctcaagctc accggcatga ccagagagct tttcaaaagc acctgcttcg tgggtcaaag
     1981 agaactggat gaacacgcct tcggcggcga cgatatggct tccctcgtgc agggcatcgc
     2041 cgaccgggcg acgctggcgg ccgcgagcct gatcgatgcg agcaacgacg agatcaccaa
     2101 ggcggttaac ggcttcatcg ccgacgccaa gacggccgag aaggtcggat cgatcgacaa
     2161 ggtcaccgtg agcttcc
//
LOCUS       DAOQNZ010000698         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32181_length_2176_cov_3.613390, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000698 DAOQNZ010000000
VERSION     DAOQNZ010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32181_length_2176_cov_3.613390"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="PKW73_12300"
     CDS             <1..1426
                     /locus_tag="PKW73_12300"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNA74116.1"
                     /translation="PHRVGEINKFGRHRPREARVASVFGVDVELLKKNYGSPLFVTSE
                     ERLRANVRRIKKAFAVRWGNTCHGWSYKTNYISAVCNILHQEGSLAEVVSAFEYEKAR
                     ALGVPGEMIIFNGPNKSRAILERAVAEGAQIHLDHLDELNLVEAVAAAQGRKARVALR
                     LNFDTGYSEPWSRFGFNLESGQARQAAKLAAASPHLQLCGLHSHLGTFILEPRAYGEQ
                     VKIMCKFMREIESSEDVQIDVIDIGGGLPSMNALQSIYLPPEQVVPSINEYAEVICGT
                     LIEETRYRSHTNRGLPKLVFESGRAVVDDAQVLITSVVGTKRLPDGRAAAVLDAGTNL
                     LFTAYWYNHKVETTGEARGALADTVLFGPLCMNIDVVRHSVQLPPLRAGDSLVVSPVG
                     AYNNSQWMQFIEYRPNVVLVHEDGDVSVIRAAEDLSVMMAQDRLPEHLATPFAPVRSA
                     GCNRFDDAAETQAKTLRPFRICRG"
     gene            1523..>2176
                     /locus_tag="PKW73_12305"
     CDS             1523..>2176
                     /locus_tag="PKW73_12305"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNA74117.1"
                     /translation="MKLLLGTWSAFRVVLTGYGSFFGLKHPLGQFLLLAASLLHPFTG
                     LAGLLCGSMVMFFRHLLQFSREGDQLDIVNGLLLGMLIGSLYSPSVAYFVLLLTGALL
                     VVLSSAALADVLGRLFRLPVLGMPYVMSAYLLLPLMSMLSKMPVSMAALHLEPLYVVP
                     TLPVPPALDCLYSMPLAGTFSALGSMYFSGTPLGGILVFSAFLVSSRYLCLLAFAAAT
                     "
BASE COUNT          412 a          598 c          617 g          549 t
ORIGIN      
        1 gccccaccgg gtcggggaaa ttaacaagtt cggccgccat cgcccccgtg aagcccgggt
       61 agcctcggtc ttcggcgtag atgtagaact tttgaagaag aactatggtt cgcccttgtt
      121 cgttacttca gaggaaaggc tgagagccaa tgtgcgccgc atcaagaaag cctttgccgt
      181 gcgctggggc aatacctgcc atggttggtc ctacaaaacc aattacataa gcgccgtttg
      241 caatattttg caccaggaag gctctctggc tgaggtggtt tccgccttcg aatatgagaa
      301 ggccagggcc ctcggtgtgc ccggcgagat gataattttc aatggtccaa acaaaagtcg
      361 tgccatttta gagcgcgctg tagccgaggg ggcccagatt catctggatc acctggatga
      421 gctgaatctg gtggaagccg tggctgccgc tcagggaagg aaggcccggg tggctctccg
      481 tcttaacttc gacacaggct atagcgagcc ctggagtcgc ttcggcttca atctggaaag
      541 cggtcaggcc cgccaggcgg ccaagttggc ggcggcaagc ccccatttgc agctttgtgg
      601 actgcacagt catctcggca cctttattct agagccgcgc gcctacggcg agcaggtcaa
      661 gatcatgtgt aaattcatgc gcgagataga atcaagtgag gatgttcaga tagatgtaat
      721 cgacatcgga ggcggcttgc cgtccatgaa tgctctacag agcatatatt tgcccccgga
      781 acaggtagtg ccttccatca acgaatacgc cgaagtaatc tgcggcactc tcatagaaga
      841 aacgcgctac agaagccaca cgaaccgggg cttgccgaag ctggttttcg agagcggcag
      901 agccgtagtg gacgacgctc aggtgctcat cacctccgtg gtgggcacca agcgtttgcc
      961 cgacggacgt gccgccgccg ttctggatgc cggtaccaat ttgctgttca cggcttactg
     1021 gtacaaccat aaggtagaaa ccacgggcga ggcccgcggt gctctcgccg atacggtgct
     1081 cttcggtccc ctctgtatga atatcgatgt ggtgcgccat tcggtgcaac tgccgccgct
     1141 cagggccggc gacagtctgg tagtcagtcc tgtcggcgcc tataacaaca gtcaatggat
     1201 gcagtttatc gagtacaggc caaacgtggt tctggtgcat gaagacggcg atgtttcggt
     1261 gataagggcg gctgaggatc tctcggtgat gatggcccaa gatcgcctgc cggagcattt
     1321 agccacaccc tttgctcctg taagaagcgc cggctgcaac cgtttcgacg acgccgccga
     1381 aacgcaggca aagaccttaa gaccgtttcg aatttgtaga ggttaggaaa tgcaaacact
     1441 gagcgaaaaa ttgccattgc cggcggggcg gttgctattt cccattcccc ccccttttag
     1501 gggtattgcc ggatttccac ccttgaaatt actgctggga acctggtctg cttttcgggt
     1561 ggtgctgacc ggttacggct cattctttgg attgaagcat ccgctaggcc agttcttgct
     1621 tcttgccgcc agccttttgc atccctttac cggtctggcc gggctgctgt gcggctcgat
     1681 ggtgatgttc tttcgccatc ttttgcagtt ctcccgagaa ggcgaccaac tggatatagt
     1741 caacggtctc ttgcttggca tgttgattgg ctctctctac agcccttcgg tggcatattt
     1801 cgtattactt ctgaccggcg ccctgctggt ggttctgagc agtgccgctc ttgccgatgt
     1861 gctggggcgt ctttttcgtc tgccggtgct gggtatgccc tatgttatga gcgcttatct
     1921 gctcttgccg ctcatgagca tgctttcgaa aatgcctgtt tccatggcgg cgcttcatct
     1981 ggagccacta tatgtagtgc cgaccctgcc tgttcctccg gctctcgatt gtctgtattc
     2041 gatgcccctg gccggtacct tttcagccct cggttccatg tatttcagcg gcacgcctct
     2101 ggggggcatt ctggtcttca gcgccttcct ggtttcttct cgttaccttt gtctgcttgc
     2161 ttttgcggcg gctact
//
LOCUS       DAOQNZ010000699         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32229_length_2174_cov_4.460595, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000699 DAOQNZ010000000
VERSION     DAOQNZ010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32229_length_2174_cov_4.460595"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..866
                     /locus_tag="PKW73_12310"
     CDS             156..866
                     /locus_tag="PKW73_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74118.1"
                     /translation="MPVARAAQLLGESIDSLEYRLERGEIRGEKRQISERHSGKDCWF
                     IYACDFEALLDVRVKACQTRLKTSPVQEERLTVEGLDPIFSVEAEILPEPQEIPIDQN
                     QASFTLPQEVPASMSACTVSAEPQEEPMASLSEEAATINLHFQTGSDNQNEAEQQQRD
                     MLMPVATELWNQIQKGEEEKQQLRLRLNQLSQEIQALHEEMSLLKIEVLGMNPGGKNC
                     STSGKVSLLARFKRFFGL"
     gene            1225..>2174
                     /locus_tag="PKW73_12315"
     CDS             1225..>2174
                     /locus_tag="PKW73_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNA74119.1"
                     /translation="MSTEPVKYNGVPVPAGETISFLAGKPVTPDCPIIPFIEGDGIGS
                     EIWGATRLVLDAAIARAYGSKRRIAWLEVFAGDKARAQFGKALPDDTLAAIKAFGVAI
                     KGPLNTPTGGGMRSLNVTMRQTFDLYQCVRPVRYFPGVPSVVKSPQDLDVVIFRENTE
                     DVYAGIEFKIDSDGAKRVISLLDEMGIKVLADSGIGIKIMSRMGSRRLVRRAIQYAID
                     HNRKVVTLVHKGNIQKFTEGAFRDWGYELAREEFADLIVTEADLWAKHNGVLPEGKIL
                     LNDRIADAMFFEVLTKPNKFSVIATMNLNGDYLSDACAAQV"
BASE COUNT          548 a          632 c          538 g          456 t
ORIGIN      
        1 ccacctttag caggtgctat atacagggct ttgacaatta tatatacaac gcacgacctt
       61 ttgcaggcgg ctccgtatat ggtatcaatc ccagaattgc gagtacctag acattgctgc
      121 gagtagatac ccaggctatt aagatacacc ctctcatgcc ggttgcgcgt gccgcccaac
      181 tgcttggaga gagtatcgac agtctcgaat accgccttga aagaggggaa attcgcggcg
      241 agaagcggca gatttcagaa agacacagcg gcaaagactg ctggttcatc tatgcctgcg
      301 acttcgaggc ccttctcgat gtgagagtaa aagcctgcca gaccaggctg aaaacaagcc
      361 ctgtccagga agaacggttg accgtcgaag gtcttgatcc tatcttctcc gtagaagccg
      421 agattttgcc ggaaccgcag gaaataccaa tagaccaaaa ccaagcaagc ttcacactcc
      481 ctcaggaagt ccctgcttca atgtcggctt gcaccgtatc cgccgaacca caggaagagc
      541 ctatggcttc cttaagcgaa gaagcggcta ctatcaatct gcacttccag accggttcag
      601 acaaccaaaa cgaagcggaa cagcaacagc gcgacatgct catgcccgta gcaaccgaac
      661 tctggaatca gattcagaaa ggcgaggaag agaagcagca actgcgtctg cgactgaatc
      721 agctcagcca ggaaatacaa gccctgcatg aagaaatgag tctgctaaaa attgaagtgc
      781 tggggatgaa ccctggcgga aagaactgct ctacttcggg caaagtgagc ctattggcac
      841 gcttcaaacg cttctttgga ctctaaacaa accctatcgt accgccgctc acaagacgct
      901 tgcgctagcc tcgcctactt tacaaccaga tcttttgaac tgctttctac gcctccctgt
      961 atgaattctc aaaaaatcac cctgataaag cctattgaca acaattgaga ggttgcgccg
     1021 tcggccgggg cggccttagg ttgtgacgtt aattccgccc gtaattttca ccgctgttta
     1081 tagcttctaa aagtgtcttt cctagtttct acgttcatga gcgagatttt tcaatcttgc
     1141 agattggcac gaagaaaatt cgacggtcac cgcagaggct ctaaccaaca taacctgaac
     1201 ctgttcagaa gaaggcaaca agctatgagc acagaacctg tcaaatacaa cggtgtacca
     1261 gtaccagccg gcgaaaccat cagtttcctg gcaggcaaac cggtcacgcc tgactgtcct
     1321 atcattccgt tcatcgaagg cgatggtatc ggttcggaga tctggggtgc cacccggctc
     1381 gtcctcgatg cagccatcgc ccgcgcctat ggcagcaaac gccgcatcgc ctggctggaa
     1441 gttttcgccg gtgacaaagc cagagcccag ttcggcaaag cgcttcctga cgacaccctg
     1501 gctgccatca aggccttcgg cgtggccatc aagggaccgc ttaatacccc caccggtggt
     1561 ggcatgcgca gtctcaacgt gaccatgcgc cagaccttcg acctttacca gtgcgtccgc
     1621 cccgtgcgct acttccccgg cgtgccctcc gtggtcaagt cgccccagga cctcgacgtg
     1681 gtcattttcc gcgaaaatac tgaagacgtc tacgccggca tcgagttcaa aattgactcc
     1741 gacggtgcca agcgtgtcat cagtcttctc gacgagatgg gcatcaaggt tctcgccgac
     1801 tccggcatcg gcatcaagat catgagccgc atgggctctc gtcgactggt acgccgtgcc
     1861 attcagtacg ccatcgacca caaccgcaag gtggtcaccc tggtgcacaa gggcaacatt
     1921 cagaaattca ctgaaggggc cttccgcgac tggggctacg agcttgccag agaagagttc
     1981 gccgacctga ttgtcacgga agccgacctc tgggcaaaac acaacggcgt cttgccggaa
     2041 ggcaagattc tgctcaatga ccgtatcgcc gacgccatgt tcttcgaagt gctgaccaag
     2101 cccaacaagt tctcggtgat tgccaccatg aacctgaacg gtgactacct ctcggacgct
     2161 tgcgccgctc aggt
//
LOCUS       DAOQNZ010000700         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32265_length_2173_cov_3.848914, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000700 DAOQNZ010000000
VERSION     DAOQNZ010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32265_length_2173_cov_3.848914"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1375)
                     /locus_tag="PKW73_12320"
     CDS             complement(107..1375)
                     /locus_tag="PKW73_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNA74120.1"
                     /translation="MNARTKLNAALTLTLLNLLSATVPLALTFAPFTGEPFPSNHSAL
                     AAEKVNLDSYMKKVSDKIGPLWNPSKTEAKKLTVKFTINANGSVADLAVDEPTGDKAL
                     EEQALALIKKAAPFAALPPGMPKLTVSFGLPTQRGVTASGVNMQSYYDDMWKHIQAGW
                     WVPKRLLFIRVPLKIVINKDGSLNAVSLIKSSGDANADKLALVGVRRAAPFAPLPEGV
                     DAPFTVNYTIGYDGSKDHFVIWNGQKVNMGDSYTSAGGVKTTLKDTTTEKDKQFHLRK
                     ENALIKMSELDDVIAAKEKQGGSENQELAPILLEYAKQHQSIEEQKQALEKLRRALKI
                     CEAAPEKKAELLRCLVVVGEMEYSLGNLKEAETLLTRAIAMKEEAIKVNPSEKDADYK
                     SLLNTYARLLYKQSRQKEADEQYKKIKELG"
     gene            complement(1405..>2173)
                     /locus_tag="PKW73_12325"
     CDS             complement(1405..>2173)
                     /locus_tag="PKW73_12325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA74121.1"
                     /translation="VDHFTFKAAGQQRLELEKNNLLFKLLNQQEELKNQLARDLHDSV
                     IADLMMLKRYLSGDRKLSTEETIEIVDEIVLQLRDIVNEYSPRQLQEWGLKVGIEDLL
                     DRMTRRTGIETQLNFQGELPRFPDLVTLHLFRIIQESLNNVEKHAGATRVTVQIQASK
                     FGDCVFTIEDNGRGFEPEKVRVEADGSHSMGLEGMKERVELIRCFYRADLKIFSRKAR
                     LPEEQFLLRDTTSADIADSTESPSESGTRIDLIIRAQ"
BASE COUNT          425 a          547 c          572 g          629 t
ORIGIN      
        1 ttagtagtgt cttttcccag ttggaaaata ctacttttgc tatgagacat ttttggcacc
       61 tctggctact aaagcatgct tagtttatgc cggaagtggt caaaccctag cccagttcct
      121 tgattttttt atattgttca tccgcttctt tctgacggct ttgcttgtag agcaatcggg
      181 cgtacgtgtt caagaggctc ttgtaatcag cgtccttttc ggacggatta accttgatcg
      241 cctcttcctt catggcaatg gccctggtga gaagagtttc cgcttctttg agattaccca
      301 gagaatattc catttcaccc acaaccacga gacagcgcaa tagctctgct tttttctcgg
      361 gggcggcttc gcaaattttg agggcccgcc gcagcttttc cagggcctgt ttctgctctt
      421 ctatagattg atgctgtttg gcgtattcca acagaatcgg cgccagctct tggttctcgc
      481 tgccgccctg cttctccttg gcggcaatga cgtcatccag ctcggacatt ttgatgaggg
      541 cattttcctt gcgcaagtga aattgcttgt ccttctccgt ggttgtatct ttgagggtgg
      601 ttttgactcc gcctgcggaa gtgtagctgt ctcccatatt tactttctga ccgttccaga
      661 tcacgaaatg atctttggaa ccgtcatagc cgatggtgta gttgaccgtg aaaggcgcat
      721 ccacgccttc cggcaagggc gcaaagggag ccgctcgcct cactcccaca agggccagtt
      781 tgtcggcatt ggcgtcgccg gatgacttga tcaggctgac ggcattcagg gagccgtctt
      841 tattaataac tattttgagg ggcacccgta tgaacaaaag gcgtttgggc acccaccaac
      901 cggcttgaat gtgcttccac atatcgtcgt aatagctttg catgttcaca ccgctggcgg
      961 tgacaccgcg ctgggtgggc agaccgaagc tgactgtcaa cttgggcatg ccgggaggta
     1021 aggcggcgaa aggagctgcc ttcttaatca aggccagcgc ttgctcttcc agggccttgt
     1081 caccggtagg ctcgtcgacg gccaggtcgg caaccgaacc gttggcattg atggtaaatt
     1141 tcaccgtcag ctttttcgcc tcggtcttgg acggattcca gaggggaccg attttgtcgg
     1201 agaccttctt catgtaggaa tcgaggttta ccttttcggc ggctagagcc gaatggttcg
     1261 aaggaaaagg ctcaccggtg aagggggcga aggtcagggc cagtggtacc gtagcgctga
     1321 gcaaatttag aagggtgaga gtcagtgccg cattcagttt ggtacgagca ttcacgattg
     1381 ttttttcctt tgtttaccgg tcggttactg ggctctgatg atcaggtcta ttctggtgcc
     1441 gctctctgac ggcgattcgg tcgagtcggc aatatctgcc gatgtcgtat cccttaaaag
     1501 gaattgttct tccggcaagc gagctttccg ggagaatatc ttgagatcgg ccctgtaaaa
     1561 gcagcgtatt aattctaccc tttctttcat gccttccaga cccatggaat gtgaaccatc
     1621 ggcttccact cttacttttt ccggttcgaa gccacgcccg ttgtcttcaa tggtgaagac
     1681 gcagtcgccg aatttggacg cctggatttg cacggtgacc ctggtggctc cggcatgctt
     1741 ttctacattg ttcagacttt cttgaatgat gcggaagaga tgcagtgtga ccagatccgg
     1801 gaaacgcggc agttcaccct ggaaatttag ctgggtttct ataccggtgc gcctggtcat
     1861 gcgatcgaga aggtcttcga tgcccacttt tagtccccat tcctgcaatt gtctgggcga
     1921 atactcgttg actatgtcgc gcaattgcag cactatctcg tccacaattt caattgtctc
     1981 ttccgttgaa agtttgcgat ctcccgacag gtagcgcttc agcatcatca ggtcggcgat
     2041 gacgctgtca tgcaggtctc gtgctaattg gtttttcagt tcttcttgct ggttgagcag
     2101 tttgaagagc aggttgttct tttccagttc cagtctttgc tgcccggccg ctttaaaggt
     2161 gaagtggtca acc
//
LOCUS       DAOQNZ010000701         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32279_length_2173_cov_2.908404, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000701 DAOQNZ010000000
VERSION     DAOQNZ010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32279_length_2173_cov_2.908404"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1042)
                     /locus_tag="PKW73_12330"
     CDS             complement(236..1042)
                     /locus_tag="PKW73_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA74122.1"
                     /translation="MSQTNSSQFKVEVTDVCKRYNIYDKPVDRLKELVTRNRLCCHRE
                     YWALRDINVQFPKGVTALIGPNGSGKSTLLQIIAGVLEPTSGTVERRGRITAILELGA
                     GFQPDYSGRENVILNGMILGISREEMETRIESIAEFAEIGDFFDQPIKTYSSGMVVRL
                     AFATAVSVEPEILLVDEAMAVGDQNFQTKCRDHIWKMKQSGKTIIFVTHDMDMVRSWC
                     DQAVLLNGGQTVKVGDVNEVVEAFEDLMNHHEKVAVKRFTAPAEAAKAGV"
     gene            complement(1138..1503)
                     /locus_tag="PKW73_12335"
     CDS             complement(1138..1503)
                     /locus_tag="PKW73_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNA74123.1"
                     /translation="MKWNVLPGKTLSLALSLVFACGLLLFENCEPAQAQGVPGAAVGS
                     DMGSAAGGGGQMRGKHRRGHRGGGKGHKGGGRAKMMQRFDTNGDGKLDEAEKANLKKF
                     RAERKAQRQAMQNDGSRRH"
     gene            complement(1655..>2173)
                     /locus_tag="PKW73_12340"
     CDS             complement(1655..>2173)
                     /locus_tag="PKW73_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNA74124.1"
                     /translation="LGDLFAYHEQKNARVTMVSVPMLSPYGVVDFNDRGQVTAFREKP
                     ELPYWVNAGIYVVDPSIYEQLPDRGEHEQFTFPLLAEKGQLHAFKSTAFWRSINTVRD
                     IGELRADMEKLFFSLFFSPVVSYAANQAVKQASVGHAFNAQTVAPRAVVEPPSEEREE
                     VPMVSVNRISLV"
BASE COUNT          510 a          615 c          530 g          518 t
ORIGIN      
        1 gcataggttt gcaaaatttc cacactacca tcggtagacc cgtcatccac ggcaattatt
       61 tcaagatctt tgtacgactg cgcaagcaaa ctttctacag cctgattcag gaaggcagcg
      121 ccgttgaaca ccggaagaag tacggaaacc agatctgcca taaaattctt tctactgtct
      181 attcaaccga gtgagatgtt gcatgctgtt gttcaacacc ggaacagatg aaggcttaga
      241 caccggcctt agcggcctct gccggagccg taaagcgctt cactgctact ttctcgtggt
      301 gattcatcaa atcttcgaag gcttccacta cttcgttgac atcgccgacc ttgacagttt
      361 gaccgccatt caatagtacg gcttgatcac accaggagcg aaccatgtcc atgtcgtggg
      421 taacgaagat aatggtcttg ccggactgtt tcattttcca gatgtggtcc cggcacttgg
      481 tctgaaagtt ctgatcacct acagccatgg cttcatccac aagcagaatt tcgggttcga
      541 cactaacagc ggtggcaaag gccaggcgca ctaccatacc gctggaatat gtcttaatgg
      601 gctgatcaaa gaagtcgcct atttcggcga attcagcaat ggattctatg cgcgtctcca
      661 tttcctcccg gcttatgccc aggatcatgc cgttcaaaat tacgttttct ctgccgctgt
      721 aatccggctg aaaaccggca cctaattcca agatagcagt tatgcgaccg cgtctttcta
      781 cagtgccgga agtcggttcc agcaccccgg cgataatctg cagcagggta gacttaccgg
      841 agccgttagg accaatgaga gcagtcacac ctttggggaa ctgcacgttg atatcccgca
      901 aagcccagta ctcgcggtgg cagcaaagtc gattgcgtgt aacaagctcc ttgagcctat
      961 ctacgggctt atcgtagatg ttatagcgct tgcatacatc tgttacttca actttgaatt
     1021 ggctggaatt ggtctggctc atggttccac aaaagacaac gcagttccca agatgttagc
     1081 acatcaggac tcgatcccca tgagaagggt ccaatcagca ctttacagga ccggctctca
     1141 gtgcctgcgg ctgccgtcgt tttgcatggc ctggcgctga gccttgcgct cggctctgaa
     1201 cttcttgagg ttggcctttt ctgcctcatc cagctttccg tcgccattgg tgtcaaagcg
     1261 ctgcatcatc ttggctcgcc caccgccttt gtgcccttta ccgccacctc tatgaccgcg
     1321 ccggtgctta cccctcatct gaccgccgcc accggcggca ctgcccatat cggagccgac
     1381 agccgcccca ggaacccctt gagcctgagc cggctcacag ttctcaaata agagcagccc
     1441 acaggcaaaa accagtgaga gggccagact cagcgtcttt ccaggcagaa cgttccattt
     1501 catcgcgctt acctcagttc aaaggagatg ccccagacta ccctcaggca atctaccctt
     1561 tcataaacgt aattgcgcgc taaaaagttc aatgcaactg ccgggaaatg cccttgcact
     1621 tcattaaact ttcttagcct gaccttttga ggatttaaac cagggaaatg cggttaacgc
     1681 taaccatcgg cacttcctct ctttcctcgg agggcggctc tacaaccgcc ctgggggcga
     1741 cggtctgggc attaaaggca tgaccgacac tggcttgctt cacggcctga ttagcggcat
     1801 aactgaccac tggagagaag aagagcgaga agaagagctt ctccatgtcg gctctcagtt
     1861 cgcctatatc acgcacagtg ttgatggaac gccagaaggc cgtgctcttg aaggcatgga
     1921 gctgaccttt ctcggccaga agcgggaagg tgaactgttc gtgttcgcca cggtcgggta
     1981 gctgctcgta gatggaaggg tctaccacat aaataccggc attgacccag tagggcagct
     2041 ccggcttttc acggaaagcg gttacctgcc ctctgtcatt gaaatcaacc acgccgtagg
     2101 gactgagcat gggtaccgaa accatggtca cacgagcatt cttctgttcg tgataggcaa
     2161 agagatcgcc cag
//
LOCUS       DAOQNZ010000702         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32341_length_2170_cov_4.270449, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000702 DAOQNZ010000000
VERSION     DAOQNZ010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32341_length_2170_cov_4.270449"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..478)
                     /locus_tag="PKW73_12345"
     CDS             complement(197..478)
                     /locus_tag="PKW73_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74125.1"
                     /translation="MSTMLGQYSNSVMPTTGTAELLSVSESGAFVVSLPPALKAHQWE
                     LENLLGKFFNGRPTSAGNLSLARQLAINWCISKCKQTGQSVDKCLEHLS"
     gene            892..1395
                     /locus_tag="PKW73_12350"
     CDS             892..1395
                     /locus_tag="PKW73_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNA74126.1"
                     /translation="MVENEFSQGKPENEVGKEGSSVLEMRHSEDSHVRRIVAGNPNTP
                     LAVLTRLADDLSYHVRRAVAENPRSGDALLMYLASDGSSEVRMAVAENRNTNLLTLRQ
                     LAEDGCDDVRYGMAECPHVPAEILLRLADDENPYVRFRSMKTMQLLGLPGKLCLLTGR
                     PLHRLYG"
     gene            complement(1465..2166)
                     /locus_tag="PKW73_12355"
     CDS             complement(1465..2166)
                     /locus_tag="PKW73_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA74127.1"
                     /translation="MVSACEVCPLKILIIEDDVNIARLIELELGYEGYQVSVAHDGNQ
                     GLDLFKKDQPDLVLLDVMLPGLDGIEVCKRLRGMSKVPVIMLTAKDGVQDRVSGLDSG
                     ADDYLTKPFATEELLARIRAVMRRKPQEGILTYADLWMDQLNRIVKRGERQLDLRAKE
                     FDLLRLFMQYPEQVLSREFIFDKVWGYDFIGESNVIEVYIRYLRSKLEDASSKRLIQT
                     VRGEGYVLREEEPSR"
BASE COUNT          492 a          534 c          589 g          555 t
ORIGIN      
        1 ccggtgtacg cttgcagcaa ctgggtgagg atgattcggt ggtctcggtg acacctattg
       61 tggaaccctc tccggatgcg gaccagggcg ataacggcgc tgaataagag cgtctttgcc
      121 gcgcgatcaa ttcaaaaggc gtgacctcag taggtcacgc cttttggcta aaagtacaaa
      181 gccggtttcg gggcactcag gagagatgct cgaggcactt atctactgat tggcctgttt
      241 gtttgcattt ggaaatgcac cagtttatgg ccagctgtct ggccagcgaa agattgccgg
      301 ctgaggtggg tctgccgttg aaaaacttgc cgagcagatt ttcaagttcc cactgatggg
      361 ccttgagtgc aggcggcagg ctgaccacga aggcaccact ttcggacact gaaagaagtt
      421 cggccgtacc ggtggtcggc ataactgaat ttgaatattg gcccagcatc gtactcatgt
      481 gattcctcgt tcttcggcgt ttttggaagg caagtactaa taattgaatt tgcaagagaa
      541 cggtatttga atgactcttt gcaagttctt ggcagtgtaa gttgctttct gaaacatacc
      601 taaactatat accgttatca taacgttacg tcatgttttt ttacaacccc ggtaattccc
      661 atattaggct gattttggca cttggaaatg ggaatattcc caggctttcc ccttggcagt
      721 tttgtggttc ggcaaagttt caacaaagac cgacctgatg atcctcaggg tcacccgtgg
      781 tttcagcaaa ttcaccctta aggttgacgg taccgcgccg ccggtcctgg gcataaacag
      841 tcatcaggtg ctggttttgg ttccaaattt taaaatttaa gaggtcggaa aatggttgag
      901 aatgagtttt cgcaagggaa gcctgagaac gaagttggaa aggaaggttc ttcagtcctg
      961 gagatgcggc attcagagga ttcacacgtc agaaggatag tagccggcaa cccgaacaca
     1021 ccacttgcgg tgctgacccg actggctgac gatttgtcat accatgtgcg ccgggctgtg
     1081 gctgagaatc ctcgtagcgg cgatgcttta ttgatgtacc tggcctcgga cggcagttcg
     1141 gaggtacgta tggctgtggc cgaaaaccgc aataccaatt tgcttactct gcggcaacta
     1201 gccgaggatg gttgtgacga cgtccgctac ggtatggccg aatgccccca tgtgccggcc
     1261 gagattctct tgcgtctggc ggacgacgag aatccctatg tgcgttttcg ctccatgaaa
     1321 accatgcagc tacttggact gcccggtaaa ctctgtctct tgacggggcg ccctctgcat
     1381 cggctctacg gttgaagctc agagaggcat gacaaaaagc cgcccctcct gacaggggcg
     1441 gctttccttt ggcaatttgc cgttttagcg ggagggctct tcttctctca gcacgtagcc
     1501 ttcaccacgc acggtttgaa tgaggcgctt ggagctggca tcttccagtt tggagcgcag
     1561 gtaacggatg tagacttcga tgacgtttga ttcgccgatg aagtcatagc cccatacttt
     1621 gtcgaaaatg aactcgcgtg aaagcacttg ctcgggatac tgcatgaaca gtctgagcag
     1681 gtcgaattct ttggctctca gatcaagctg acgctcgcct cttttgacga tacggttcaa
     1741 ttggtccatc cacaagtcgg cataagtaag aatgccttcc tggggcttac ggcgcatcac
     1801 tgcgcgaatt ctggccagga gctcttctgt ggcaaagggc ttggtcaagt agtcgtcggc
     1861 accactgtcg aggccgctga cgcggtcttg cacgccgtct ttggcggtga gcatgatcac
     1921 cggaacttta gacatgccgc gcagacgctt acaaacttca atgccgtcca gtcctgggag
     1981 catcacgtcg aggagaacaa ggtcaggttg gtctttcttg aaaaggtcga gaccttgatt
     2041 accatcgtga gccacggata cttgatagcc ttcataaccc agctctagct cgatgagacg
     2101 agcgatgttt acgtcgtctt caatgatcag gattttcaac ggacatacct cacatgcact
     2161 taccacggtt
//
LOCUS       DAOQNZ010000703         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32342_length_2170_cov_4.211348, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000703 DAOQNZ010000000
VERSION     DAOQNZ010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32342_length_2170_cov_4.211348"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKW73_12360"
     CDS             complement(<1..397)
                     /locus_tag="PKW73_12360"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNA74128.1"
                     /translation="MQIDGVIVKKLTQISDERGKIMHMMRLDDPLFKGFGEIYFSQVY
                     PAVVKGWHLHSQMTLNYAVVSGTIKLVLYDDRSDSASRGTLQEIFMGESDYKLVQVPP
                     GVWNGFKGVGTAPALVANLASHPHSADEIS"
     gene            complement(417..1211)
                     /gene="rfbF"
                     /locus_tag="PKW73_12365"
     CDS             complement(417..1211)
                     /gene="rfbF"
                     /locus_tag="PKW73_12365"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019112401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNA74129.1"
                     /translation="MKVVILAGGLGTRLQEETQVKPKPMVEVGGRPVLWHIMKIFAHY
                     GFNEFVIALGYKGGVIKEYFLNYRHRDSDLVVKLKSGAVTVKNDGDVEDWTVHLIDTG
                     LASMTGGRIKRASQYIGDEPFLVTYGDGVADVDIGAVVDLHKKQGKKATVTAVRPPAR
                     FGEIYFGEHETGPCTVSRFEEKPQTESGWINGGFFVLEPSIRDYIDSDASIFEREPLQ
                     RLAAEGELGSYLHYGFWQCMDTVRDVETLQKLWAQGEAPWCLWQKK"
     gene            1295..>2170
                     /gene="rfbH"
                     /locus_tag="PKW73_12370"
     CDS             1295..>2170
                     /gene="rfbH"
                     /locus_tag="PKW73_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNA74130.1"
                     /translation="MSDTTPTSKQEAQTLKKEILSLVAKYQKLNWPEEGKSHQPGDFV
                     PYAGRVFDEEELVNLVDASLDFWLTTGRFARQFETEFAAYLSRRYALLCNSGSSANLL
                     AVSALTSYRLKERQLKPGDEVITVAAGFPTTVAPIVQNRLVPVFIDVDLPTLNADTVY
                     LEEALSNKTRAIILAHTLGNPFDLDAVLAFAEKHNLFVIEDNCDALGSLYKGQLTGSR
                     GDLSTSSFYPAHHITMGEGGCVTTSDPKLKVIVESFRDWGRDCYCDPGKDNTCGKRFG
                     WQLGQLPEGYDHKYIY"
BASE COUNT          536 a          628 c          504 g          502 t
ORIGIN      
        1 ggctgatttc atcggcactg tgaggatggc tggctagatt cgccaccagg gccggtgcgg
       61 taccgacgcc cttaaaacca ttccagacgc cgggcggcac ctgcaccaat ttgtaatcgc
      121 tctcgcccat gaaaatttcc tgcaaggtgc ccctgctggc ggaatcggaa cgatcatcat
      181 aaagcactaa cttgatcgtg cctgacacta ccgcataatt cagggtcatt tgactgtgca
      241 aatgccagcc ctttacgaca gcgggataaa cctgagaaaa atagatctca ccgaagcctt
      301 tgaatagcgg gtcatcaagg cgcatcatat gcatgatttt gcctcgttcg tcgctgatct
      361 gggtcaattt cttgacgatg acgccatcaa tttgcatagg tcttccttcc ccaagattac
      421 ttcttttgcc agagacacca gggagcttca ccctgagccc agagcttttg cagggtctcg
      481 acgtcgcgca ccgtgtccat gcactgccag aaaccataat gcaagtaaga accaagttct
      541 ccctcggctg ccagtctttg cagcggttcc cgctcgaaga ttgaggcatc ggaatctata
      601 taatcacgaa tggaaggttc cagtacaaaa aagccgccat tgatccaacc gctttccgtc
      661 tgaggcttct cttcaaaacg cgacaccgtg caaggtccgg tttcgtgctc cccaaaatag
      721 atttcgccga agcgagccgg tggtcgcaca gcagtcacgg ttgccttctt gccttgcttc
      781 ttgtgcaaat cgacaacggc accgatatca acgtcggcca ctccatctcc gtaggtgacg
      841 aggaaaggtt catcacctat atattgtgaa gcgcgcttga ttcgcccacc ggtcatggaa
      901 gccaaaccgg tgtcaatcaa atgcaccgtc caatcctcaa cgtccccatc attcttgacc
      961 gtaacggcac cggacttgag cttcacgacc agatccgagt ccctgtgacg ataattgaga
     1021 aaatattcct taatgacacc gcccttgtaa ccaagggcta tcacaaactc attgaaacca
     1081 taatgagcga atattttcat gatatgccag agaacagggc gaccgcccac ttcaaccatg
     1141 ggcttaggtt taacctgagt ctcttcctga agccttgttc ccagtccgcc tgccagtatt
     1201 acaactttca aattgcaccc tgattttccg ctctctgcca aaaccaaagt tgagcgactg
     1261 tataattata gcctcaaact caggggtgac atcattgtcc gacactacac caacctctaa
     1321 gcaggaagcc cagaccctca aaaaagagat cctctccctc gtagccaagt atcagaaact
     1381 caactggccg gaggagggaa agagccacca accgggagac ttcgtcccct atgccgggcg
     1441 agtcttcgat gaggaagagt tggttaatct ggttgatgca tcccttgatt tctggctgac
     1501 cacggggcgt tttgcccgcc agtttgaaac ggaatttgcc gcctatctgt caaggcgcta
     1561 cgcactgctc tgcaattccg gctcttcggc caatctcctg gcggtatcgg ccctcacttc
     1621 ctatcgcctc aaggaaaggc aactgaaacc gggagatgaa gttatcacag tggcagcagg
     1681 ctttcccacc accgtggcgc caatcgtaca aaaccgcctg gtgccggtct ttattgatgt
     1741 agacctgccc actttgaacg ccgataccgt ctatctggaa gaagcgcttt caaacaaaac
     1801 ccgcgccata attctggccc acaccctggg caatcccttc gatctggacg ccgtcctggc
     1861 ctttgccgag aagcacaatt tattcgtcat cgaagataac tgcgatgctc tcggcagtct
     1921 ctacaaaggg caattaacag gtagccgggg cgacctttcc accagcagtt tttatccggc
     1981 ccaccatatc accatgggtg aaggcggctg cgtcaccacc agtgacccca aactgaaggt
     2041 tattgtggaa tcattccggg actggggacg cgactgttac tgcgatcccg gtaaggacaa
     2101 cacttgcggc aagcgctttg gttggcaact gggtcaactg ccggaaggtt acgaccacaa
     2161 gtatatatac
//
LOCUS       DAOQNZ010000704         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32389_length_2168_cov_4.876952, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000704 DAOQNZ010000000
VERSION     DAOQNZ010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32389_length_2168_cov_4.876952"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2168)
                     /locus_tag="PKW73_12375"
     CDS             complement(<1..>2168)
                     /locus_tag="PKW73_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA74131.1"
                     /translation="YDSQDDSCPRDGGQLVPVANDPLIGTTFADKYQILKVLGSGGMS
                     VIYQARHKYMERICAVKLLHPFLVADASMFQRFQYEAKAASNLDHPNVVRVHDFGITD
                     DGRAYLVMDYLSGEDLSSILERDVILPEDKAREIFRQACMGLDHAHSKGVIHRDLKPS
                     NLFLEPQSDGSIIVKLVDFGIAKINLPGEIESGQNLTRTGEVFGSPLYMSPEQCAGLS
                     LDARSDIYSLGCLMYEVVAGKTPLIGSTAFDTMQMHLKTKPAKPTEVSPRISLSPHTE
                     AVIMQCLAKRPADRFSSVADLYEAFFQEKLPKTGLVSAGSGLQLQLKTGGEVGADQSK
                     GSTGLSKATDGKASSSKENFLHHLSRTSIARSLADPQKRPGAITAILLATILGGALIY
                     LYFGWQGPSTDRGTFFNRNRFLFAMSFADYLKDSGNFEQAEQLYNYTLEISKTFDDRH
                     AHEANALRAKLNLYEKSHNTAGTENTLAQLNGVLMKRLLEDYASAMDELDRIDTESRK
                     LNSQDGGNTVLTKQLDADLTAVIQGIIELGRRLAANQDIDRQIKLLTRACTLYNRFAA
                     SDDPQLARLKMELAICHWQRDEVETVGPLLKESLQIMKRASDNHKPGLRPLDLAEAWL
                     RLGQFDRDRSLFADAEKELDTAISLTESYEKREPRANDTRELKEIGKGRRMLTEVLNA
                     KADLEGQQKNLKEAELLRERAAKIKQLPTATKLQDNIKIL"
BASE COUNT          438 a          529 c          609 g          592 t
ORIGIN      
        1 gcaatatttt tatattatcc tggagcttgg ttgcggtggg caattgtttg attttggcag
       61 ctcgctctct tagcagttcc gcctccttca ggttcttttg ctgtccttcc aggtcggctt
      121 tggcattcaa aacttcagtg agcatgcggc gtcctttgcc tatttcttta agttctctgg
      181 tatcgttggc gcgtggttct cgcttctcat agctttcggt gagagagatg gccgtgtcca
      241 attccttttc cgcatcagca aagaggctgc ggtcccggtc aaattgacca aggcgcagcc
      301 aggcttccgc taagtcgagt gggcgtaatc ccggtttgtg attatccgag gctcgcttca
      361 ttatctgcag gctttctttc aggagcggtc ctactgtttc tacttcatct ctttgccagt
      421 ggcagatggc tagttccatc ttgagcctgg ccagttgtgg atcatctgag gcggcaaatc
      481 tgttatagag agtgcaggct ctagtgagga gctttatttg cctgtcgata tcttgattgg
      541 ctgccagtcg gcgacccagt tcgatgatgc cctgaatgac ggccgtcagg tcggcatcaa
      601 gttgtttggt gagaaccgtg ttgcccccat cttgagagtt tagtttgcgg ctttcggtgt
      661 cgatgcgatc cagttcgtcc atggcactgg cataatcttc cagcaggcgc ttcattaaga
      721 cgccgttcaa ctgtgccaga gtattttccg ttccggcagt gttgtggctc ttttcgtaga
      781 gattgagctt ggctctgaga gcgttcgcct catgggcatg gcgatcatcg aaagttttgg
      841 aaatttccag ggtgtaattg tagagctgtt cggcttgttc aaaattgcct gagtctttta
      901 gatagtcggc gaagctcatg gcaaagagga agcggttgcg attgaagaaa gtgcctctgt
      961 cggtgctcgg accctgccaa ccgaagtaga ggtaaatgag agcgccacca aggatagtag
     1021 caagtagaat cgccgtgatt gccccggggc gtttctgggg atcggcgaga gacctggcaa
     1081 tactggtgcg actgaggtgg tggaggaagt tttctttgct gcttgatgcc ttgccgtctg
     1141 tggccttgct caagcctgta gaacctttag actggtcggc gcccacttct ccgcctgtct
     1201 ttaactgcag ttgcagaccg gaaccggccg ataccaatcc ggttttcggc agcttctcct
     1261 gaaagaaggc ttcgtagagg tcggctacac tgctgaaacg atcagccggg cgcttggcca
     1321 ggcattgcat aatgaccgct tccgtgtggg gtgagaggct gatgcgaggc gagacctcgg
     1381 tcggttttgc cggcttggtt ttcagatgca tctgcatggt gtcaaaggcg gtgctgccaa
     1441 tcagtggtgt ctttcctgcc accacctcat acatcaggca accgagagaa tagatgtcgg
     1501 agcgcgcatc cagggataag ccggcgcact gctccgggct catatataga ggagaaccga
     1561 aaacttcacc ggtgcgtgtc agattttgac cgctctcgat ttctcccggc aggtttattt
     1621 tagcgatgcc gaaatcaacc agcttgacga taatggagcc gtcgctctgc ggttccagaa
     1681 agaggttgct tggtttcagg tctctatgaa tgacgccttt gctatgggca tgatccagtc
     1741 ccatgcaggc ctggcggaag atttcacgag ctttgtcttc cggcagaatt acatcgcgct
     1801 caagaatgga ggaaaggtct tcgccggaca aatagtccat gaccagatag gctctgccat
     1861 catcagtaat accaaagtca tgaaccctga ctacattggg gtggtccagg ttgctggcgg
     1921 ccttggcctc gtattgaaaa cgttggaaca tggaggcatc ggccacaaga aaaggatgca
     1981 gcagtttgac tgcgcatatt ctttccatat acttgtggcg agcctggtag atgacgctca
     2041 tgccgcccga gccaagcacc ttcaaaatct gatatttgtc ggcaaaagta gtgcctatga
     2101 ggggatcgtt tgctaccggt acaagttgcc cgccgtctct ggggcaactg tcatcctgac
     2161 tgtcatac
//
LOCUS       DAOQNZ010000705         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32461_length_2166_cov_3.029844, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000705 DAOQNZ010000000
VERSION     DAOQNZ010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32461_length_2166_cov_3.029844"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKW73_12380"
     CDS             <1..313
                     /locus_tag="PKW73_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74132.1"
                     /translation="NSVEEYNYKQDKICPVGSVIGSAANAGATPAYLPEAYICGVVER
                     TQVFENPFTHYYFQWALVTVGKVQFDVCIDAATLDIPMVAGGVVAGDFHLSGQLILDG
                     A"
     gene            complement(322..1668)
                     /locus_tag="PKW73_12385"
     CDS             complement(322..1668)
                     /locus_tag="PKW73_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74133.1"
                     /translation="MPEVGGVMSLGLYLKGRFSPKNKDGEEVSLPVFDRGKLEEFERW
                     ISNVAFDEMEWSQWTEDDEGNPCLMLLLHPGAEPIMLSPLADDELLVAAATSSAGPGY
                     HIFICDLLRKLGQFYGLDWYAEEESECLDETGYFETGDKARLYEEFNRWMGALSKRVV
                     ELSKEGAKFHLAMPFDGHQFDVTEPILTQLGPRDMRWLQAVIKEPSLGRDIFPWWEDG
                     HGAEYHLGRALCYMWNSLRWREPLTETEAELLETILRHLDSAYSLDPDLEFPWREWSQ
                     IIDFLGTNYELTDFVEEMADKSDLAFGRLSLVGYRRFDVRKNLGGGWSVAIPGTFVEE
                     LEEGQTFLAYDETRNVRVTCMSATDSSGNTLDAAEILRKIRVIDDPISYEKGKVLGRA
                     FFEKIEEEDATFWMLRGVSAASGTFAQVTICFEEEEEQPWALSIWRSLDHRSSHEI"
     gene            1808..>2166
                     /locus_tag="PKW73_12390"
     CDS             1808..>2166
                     /locus_tag="PKW73_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74134.1"
                     /translation="MSALKNAAVLAAALSLLVIKQAQAAPIIEEKTAGTSYMDEILRA
                     EPYKPAQAQDLKEEEGKSDRVSVSLRADGTTVETVSGNWGRIVTEKPLSTTSGLVKRE
                     TTELKKSPLPGLKRTII"
BASE COUNT          560 a          628 c          529 g          449 t
ORIGIN      
        1 taactccgta gaggaataca actataagca agacaaaatc tgcccggtgg gctccgtaat
       61 aggctcggca gccaatgccg gtgccactcc ggcctatcta ccggaagcct atatctgcgg
      121 cgtggtggag cgcacccagg ttttcgagaa tccctttacg cactactact ttcaatgggc
      181 gctggtgacg gtcggtaaag tccaatttga tgtgtgcata gacgcagcca ccctcgacat
      241 tcccatggtg gcagggggag tggtagctgg tgatttccac ttatccggtc aacttattct
      301 tgacggggcg taagccggac tctagatttc gtgactggag cggtgatcaa gagaacgcca
      361 gatagaaagg gcccagggct gctcctcttc ctcttcgaaa caaatggtga cctgggcaaa
      421 agtaccggac gccgccgaca cgccacgcag catccagaaa gtagcgtcct cttcttcaat
      481 cttttcaaag aaggcccgcc ccagcacttt gcccttttca tagctgatgg gatcatcaat
      541 gacccttatt ttgcgcagaa tctcggcagc atcaagggtg ttgccgctcg agtcggtagc
      601 cgacatgcag gttaccctca catttcgagt ttcatcgtaa gccaggaaag tctgaccttc
      661 ttccagctct tccacaaagg taccgggaat tgcaaccgac cagccgccgc ccaggttctt
      721 gcgtacatca aaacgcctgt agcccaccag ggaaagccga ccgaaagcca gatcggactt
      781 gtcggccatt tcttcaacga agtcggtaag ctcataatta gtgcccagaa aatctatgat
      841 ttgcgaccat tcccgccagg gaaactctag atccggatca agagaatagg cactgtcaag
      901 atgacgcaaa atcgtctcca acaattccgc ttccgtctcc gtgagcggct cgcgccagcg
      961 cagggaattc cacatatagc aaagtgctcg gcccaggtga tattcggcac catggccgtc
     1021 ttcccaccag ggaaagatat cgcgccccag ggagggttct tttatgacag cctgcaacca
     1081 gcgcatgtca cggggtccga gctgggtcaa aatgggctcg gtcacatcaa actgatgacc
     1141 atcgaaaggc atggccaggt ggaacttggc cccttccttg gaaagctcca ccacgcgttt
     1201 actgagagcc cccatccagc gattgaactc ttcgtaaagc cgggctttgt cgccggtctc
     1261 aaaatagcct gtttcatcca gacactcgga ttcttcttcg gcgtaccagt ctaaaccgta
     1321 aaactgcccc agcttccgca aaaggtcaca aataaagatg tgatagccag gtccagccga
     1381 ggaagtggca gcggcaacca gaagctcatc atcagccaga ggggaaagca ttatcggttc
     1441 ggcccccgga tgcaacaaaa gcatcagaca gggattacct tcatcgtcct cggtccactg
     1501 ggaccattcc atctcatcaa aagcaacgtt ggagatccag cgttcaaatt cttccagctt
     1561 gccgcgatcg aaaactggta aggaaacctc ttccccatcc ttattcttag gggaaaacct
     1621 gccttttaga tacaaaccaa gactcatgac acctccaacc tctggcattc tactacttca
     1681 gctctcaggc gcacaggcat ggcgccagat ttggccgccc cccccttggc aagacaactt
     1741 tttcgagcct cctaatttcc ctctccttcg ggttgagtct cacgttaccc agggagaaaa
     1801 gaaaacaatg tccgccctca agaatgccgc cgtactagca gccgccttaa gcctcctggt
     1861 cataaagcag gcacaggccg ccccgatcat cgaagaaaag acggcgggaa ccagctatat
     1921 ggacgaaatc cttcgagccg agccttacaa accggctcaa gctcaggatc taaaagagga
     1981 agagggtaaa agcgacagag tcagtgtcag tctgcgcgcc gacggcacca cggtggaaac
     2041 agtaagcgga aactggggac gcatagtcac ggaaaagcct ctttccacaa ctagcgggct
     2101 tgtgaagaga gaaactactg aactgaagaa atcgcccctg cccggtctca aacgcaccat
     2161 cattga
//
LOCUS       DAOQNZ010000706         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32477_length_2165_cov_3.881991, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000706 DAOQNZ010000000
VERSION     DAOQNZ010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32477_length_2165_cov_3.881991"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="PKW73_12395"
     CDS             <1..1565
                     /locus_tag="PKW73_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA74135.1"
                     /translation="LLAPLPVVNPYAGQLRLHVNCRPGTRVEETERIFKEIERAIGTV
                     VPKDEILIITDNIGMPVSGVNYAFSDSQTISEADGEILVTLKEERSKPTGQYQKEIRA
                     MLTARFPQVSYYFQPADIVTQILNAGLPAPIDIRITGLDVAKNYDIAQRLKRDVEKVR
                     GAVDVCMHQVVNAPQFVFEVDRTRANEMGLTQRDVSNSFLVTLSSSFQTAPNFWLNIK
                     NGVNYNLAAQTPQYKIDSLDAMKVTALTSKATSKKQQPPLLTNVATVSRAATPAVVNH
                     LNAQPVFDIYAACQDRDLGGVSKDIEKIMDKYRPELPQASQLTMGGQVLSMKSAFAAL
                     IFGLVFALLLVYLLLVVNFQSWSDPVIILMATPGALSGILWSLFVTQTTFSIPALMGT
                     IMTVGVASANSILMVTFANEQFHEGKDGRSAALEAGFERFRPVMMTATAMIIGMIPMA
                     IGAGQGGSQNAPIGRAVIGGLTVATFSTLFFVPLVFSLLRKGKAKDNKATEPVHNQDH
                     KQTDTNQETNHD"
     gene            1558..>2165
                     /locus_tag="PKW73_12400"
     CDS             1558..>2165
                     /locus_tag="PKW73_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNA74136.1"
                     /translation="MTEGSPKGKKHRSLHMGLRSCGLTSSVHPNRQRRSRSGMGKLIV
                     VVIIVLVIAGVLLVVGFLPRLERKKELDKMHAETTGAVPIVRTIIARPARETESLTLP
                     GNIGAIQYTTIYARVDGYLRDRMVDIGDHVRAGQLLAVIDTPTIDESLAQVRADLVRA
                     RAGVDKANAELKEAVAKQATAQAEVEKGKGNVDYATVTATRW"
BASE COUNT          558 a          621 c          553 g          433 t
ORIGIN      
        1 ggctgctggc accgctgcca gtggtgaacc cttatgccgg ccagttgcgc ctgcacgtca
       61 attgccgccc tggcactagg gtggaggaaa ccgagcgcat cttcaaagag atagagcgcg
      121 cgattggcac cgtcgtgcca aaagacgaaa tcctcatcat caccgacaat atcggcatgc
      181 ctgtaagcgg cgtcaactat gccttttccg acagccagac catcagtgaa gcagacggcg
      241 aaattctggt cacactgaag gaagagcgca gtaagcccac cggccagtat caaaaagaaa
      301 tacgagccat gttgacggcc cgctttcccc aggtgagcta ctattttcag ccagccgata
      361 tcgtcacgca aatattgaat gccggactgc ccgcccccat cgacattcgc atcaccggtc
      421 tggacgtggc taaaaattac gacatcgccc agagactcaa gcgtgacgta gaaaaagtaa
      481 ggggcgccgt tgacgtatgc atgcaccagg tggtaaacgc accacaattt gtatttgaag
      541 ttgaccgcac ccgagccaac gaaatgggtc tcacccagcg ggatgtctcc aattccttcc
      601 tggtcacatt gagctcaagc tttcagacag ccccgaattt ctggctcaac atcaaaaacg
      661 gcgtcaacta taacctggcg gcgcagacgc cgcagtacaa aatcgacagc ctcgatgcta
      721 tgaaagtaac agccctcaca tccaaggcca caagtaagaa acagcagccg ccgcttctta
      781 caaacgtagc aaccgtcagt cgggcagcta ccccggcagt ggtgaaccac ctcaacgcgc
      841 agcctgtctt tgatatctat gcggcctgcc aggaccgcga cctggggggc gtctccaaag
      901 acattgaaaa gatcatggat aagtatcgac cggagttgcc tcaagccagc cagttgacca
      961 tgggcggaca ggtcttgagc atgaaatcgg catttgccgc cctcattttt ggtctggtgt
     1021 ttgctttgct tctggtctat ttgcttctgg tggtcaactt ccagagctgg agcgaccccg
     1081 ttatcattct tatggccaca cctggtgccc tctcgggaat actctggagc ctctttgtta
     1141 cccagacaac cttctccatt cccgccctca tgggtaccat catgaccgta ggcgtggctt
     1201 ccgccaactc tatattgatg gtcacctttg ccaatgaaca attccacgaa ggcaaagacg
     1261 gacgttccgc tgcgctggaa gcaggcttcg agcgcttccg cccggtaatg atgacggcaa
     1321 cagccatgat catcggcatg atacccatgg ccataggagc agggcagggg ggctcccaaa
     1381 acgcccccat cggacgagcc gtaatcggcg ggctgacggt agcaaccttt tcgaccctct
     1441 tcttcgttcc tcttgtcttc agtcttttaa gaaagggcaa agccaaggat aataaagcaa
     1501 ccgagccggt tcacaatcag gaccacaaac aaacagatac taatcaagag acgaaccatg
     1561 actgaaggct caccaaaagg caaaaaacac cgcagtctcc acatggggct ccgcagttgc
     1621 gggctcacct catcagtgca ccctaaccgg caaaggcgca gccggtcagg catgggcaag
     1681 ctgattgttg tggtgataat agttctggtt attgccggcg ttttgctggt ggtaggtttt
     1741 ctgccgcgcc tggagcgcaa aaaagaactc gacaaaatgc atgccgaaac caccggcgcc
     1801 gtgcccattg tgcgcaccat catcgccaga ccggctagag aaaccgagtc tttgacactg
     1861 ccgggcaaca taggtgccat tcaatacacc actatttatg caagagtaga cggctacctg
     1921 cgcgaccgta tggttgatat cggcgatcac gtaagagccg gtcaattact ggctgtcatc
     1981 gacactccaa caatagacga atcactggcc caggtgagag ccgacttggt aagagccaga
     2041 gccggtgtag ataaagccaa tgccgaactg aaggaggccg tcgccaagca agccacggcc
     2101 caggcagaag tggaaaaagg aaaaggtaac gtggactatg ccactgttac tgctacccgc
     2161 tggca
//
LOCUS       DAOQNZ010000707         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32511_length_2164_cov_3.714083, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000707 DAOQNZ010000000
VERSION     DAOQNZ010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32511_length_2164_cov_3.714083"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PKW73_12405"
     CDS             complement(<1..935)
                     /locus_tag="PKW73_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNA74137.1"
                     /translation="MTDLSAFYHPAKTPADDSVDGRSAQPVESTDSVAQKLWDDTRSK
                     TSPATLTGKYQEVNFSYAPLPSLYRVKSGDTLNSIARRALNSCTEVEANNERVENAWR
                     QIARDNQIKTPKHLKPGTQLAIDGKLVSNGVEADRPKLIADMPLETYSLGGEQHRFDT
                     AKRVIEALPTGMQDFLQAAGVKLQISADFCHEAPAAAMTTPRGYGNGETNLMSPASYS
                     PITNTVHIRQTVENEKGQKFWIMPDDLAESVRHETGHALDKALGSFSRSQEFAEAYAK
                     DTSQLNEAKRKQLAYYLTDSTAGQSEEIISKPLHTT"
     gene            1178..1702
                     /locus_tag="PKW73_12410"
     CDS             1178..1702
                     /locus_tag="PKW73_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74138.1"
                     /translation="MPPDLVGDLYEPNRLSLDEALEFVANKQRVWIKSSLPREQAYLR
                     LTGMSLRVFQASTFRTMFRRAREADRLSVFLAEELTWSEKFLTDSSESVKKKLAAWDK
                     EGASGQLKKQRFLLYNLADCLEYQVRSSDPRDLAFLQKNQEVLLKIRNRLSTSSPLDL
                     KGIGEMVKSETLAP"
     gene            complement(1743..>2164)
                     /locus_tag="PKW73_12415"
     CDS             complement(1743..>2164)
                     /locus_tag="PKW73_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74139.1"
                     /translation="SAKQLGGRLKLPKPLIAASYLFMSFTTVYLFSNDEIPKTIVVLA
                     WPCCKLVALSTLVFVVLEVNRLNRELRPSINLEKYKMTARKYLAAIIFSFLLCSIPNV
                     TLRLIESILSEYVELSPVNETPNSPGDKGVGSSIGAS"
BASE COUNT          493 a          495 c          564 g          612 t
ORIGIN      
        1 gtagtgtgaa gtggcttcga aattatttct tcgctttgtc ccgcagttga gtcagtaaga
       61 taataggcga gttgctttct tttcgcctca ttcaattggc ttgtgtcttt ggcgtaggct
      121 tcagcaaatt cctgactccg agaaaagcta cccagtgcct tgtctaaagc atgtccggtt
      181 tcatgtctga ccgattcggc cagatcatcg ggcatgatcc agaatttctg gcccttttcg
      241 ttttctacgg tctgacggat atgaaccgtg ttagttatgg gagagtaaga ggccggtgac
      301 atcaagttgg tttctccgtt accgtagcct cttggagtgg tcatggctgc ggctggtgct
      361 tcatggcaga agtctgccga aatttgcaat ttgacgccgg ctgcttgtag aaaatcttgc
      421 atgccggtcg gcagagcttc gataactctt ttggcggtat cgaacctgtg ctgttctccg
      481 ccgaggctgt aggtttccag tggcatatcg gcaatcagtt tgggccggtc agcttctacg
      541 ccgttagaaa ccagcttgcc gtcaatagcc aattgtgttc cgggttttag atgctttgga
      601 gttttgatct gattgtctct agcaatttgt cgccaggcat tttctacgcg ctcattattc
      661 gcttccacct ctgtgcaact atttaaggct cgcctggcta tgctatttag agtgtcaccg
      721 cttttcaccc ggtacaacga cggcagtggt gcgtaactaa aattcacttc ctggtacttt
      781 ccggtgagag tagcgggaga ggtttttgac ctagtatcat cccaaagctt ttgagcgact
      841 gaatccgtac tctcgacagg ttgtgctgaa cgcccgtcga cgctatcgtc ggcaggagtt
      901 ttagctgggt ggtaaaatgc ggaaaggtcc gtcacggtaa agcactcgct ggtcttttcc
      961 gacgaaagta gccaaaagaa tagcagttgt cactggtgga attacggaac tcgcggcagt
     1021 cttgaagttt ggcaggcttg aaattgtctg caagtcggag gtgagcgcca tggagcgtac
     1081 cactgtttta aggcggggga cagttatctg cgcggggctt ctattcgttt ttcaatgtcc
     1141 tgcctgggcc tatcctggta gctccaagca actgcgtatg cctccggatc ttgtgggcga
     1201 tctctacgag cccaatcgtc tctctcttga cgaagcactc gaatttgtgg caaataagca
     1261 gcgagtgtgg atcaagagta gcctaccgcg cgaacaggcg tacttgcgac ttacgggcat
     1321 gtctttgcgt gtctttcagg cttccacttt tcgcaccatg tttcgccggg caagagaagc
     1381 cgataggctg agtgtatttc tggcggaaga acttacctgg agtgaaaaat tcttgaccga
     1441 cagttccgag tccgttaaaa agaagctggc tgcctgggat aaggaaggcg cttccgggca
     1501 attgaagaag caacgatttc ttctctacaa tcttgccgac tgtcttgaat atcaagttag
     1561 aagctctgac cctagggatt tggcattttt gcagaagaat caggaggtct tattgaagat
     1621 ccgcaaccgt ctttccacat ctagtcctct ggacttgaag gggattggtg agatggttaa
     1681 aagcgagacc ctggctccgt gagtttggac aaatcaaccc ttgttgcttt tgtaagttaa
     1741 gttcaactgg ctcctatact gcttccgacg cccttatcgc cgggactatt gggagtctca
     1801 tttaccgggc tcagttccac atattctgac agtatcgatt caattaatct cagcgtcacg
     1861 ttgggtatgg aacacaagag aaatgagaaa attatggctg caagatactt ccttgcggtc
     1921 attttgtatt tctcgagatt gatcgaaggg cgcaactctc ggttcaatcg gttgacttca
     1981 agtacaacaa aaacgagtgt tgatagtgca acaagcttgc agcagggcca cgcaagcaca
     2041 acaattgttt tagggatctc atcgtttgaa aagagataca ccgtagtaaa ggacataaac
     2101 agatagcttg ccgcgatcag aggctttgga agcttaaggc ggccacccaa ttgcttagcc
     2161 gact
//
LOCUS       DAOQNZ010000708         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32541_length_2163_cov_3.366698, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000708 DAOQNZ010000000
VERSION     DAOQNZ010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32541_length_2163_cov_3.366698"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKW73_12420"
     CDS             complement(<1..562)
                     /locus_tag="PKW73_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNA74140.1"
                     /translation="MNTSTGLTSVNPESTSGSGKVCAPGDKPTLASLLAPYMVHVRGE
                     RALSDNTYQAYRRDLGHFVAWFSDFQKSSRQLPKSSTSAPLSPAIPSRGDITRYLAVL
                     KKEGQVPSSLARKLASLRGWFSWLKSAGKISKDPLEAFETPHRQKKLPQVLSGPDVLS
                     LLNAAETNRDIAILELMYGAGLRVSEL"
     gene            complement(717..>2163)
                     /locus_tag="PKW73_12425"
     CDS             complement(717..>2163)
                     /locus_tag="PKW73_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNA74141.1"
                     /translation="VVRAQIQGNPDKNQMVFNSHAAADSRLKISSPYFVINQPRDEEL
                     NLDGSLTIDKAGLNLHNTNLLLGEAALKAQGQWSFVDDTVSLSILSPNPVPAKTLVGL
                     VDNNLDTKNMQGTVDGFISMEGPIKHPKLTGKISLDRVSNKDFQLYDLNGSISTDNLN
                     ASKHDPYSISVARLDIDRMRFRKLRVQDIGGLIEIQVSEPTNGDLPAPPKFLLKDITA
                     RTAGGHIKMDGHFDTEKNGLGVNIYLSQIHMEEIVDRLFDAPDELTGALDGELHITTQ
                     GTTDKQMVSNMEGTGNIVIQKGIVARFGQLQTKLTQANLLSQGILGFNINNLLQSVAP
                     ARSGEFNELTSRFQIFKGALAIKELRFSGDDLRLWGAGAADLSEQTIDLEIAGTLPRV
                     TESFLGGTIGRLSRNITVSGLLSKVTFGALENLPSLPILGDIASDKPRAFAFKVQAPA
                     QDAKLVAKSIEKTFRFLPNKQAASAHPVPGL"
BASE COUNT          463 a          523 c          608 g          569 t
ORIGIN      
        1 ccagttcaga tactctcagc ccggctccgt acatcaattc tagaatggcg atatctctat
       61 ttgtttctgc tgcattgagc aaagaaagta catccggtcc gctcaacacc tggggcagtt
      121 tcttctgcct gtgcggagtt tcaaaggctt ccagaggatc tttgctaatc ttgcctgcgc
      181 tcttgagcca ggaaaaccag ccccgcaggc tggctaactt cctggccagg ctggaaggca
      241 cctgtccttc cttctttagc acagccagat aacgggttat atcaccgcgg ctgggaattg
      301 ccggagacag aggcgccgag gtggagcttt ttggcaactg ccgcgaagac ttttgaaagt
      361 cgctgaacca ggccacaaaa tgaccaaggt ctcttcgata ggcctggtaa gtattatcgg
      421 acagggcgcg ttctccccgg acgtgcacca tataaggtgc caggagggag gccagcgtag
      481 gtttgtcgcc aggcgcacaa actttgccgg agccggaagt ggattcgggg ttgaccgagg
      541 tcaaccctgt actggtattc aattgccgat gcatgtgtat atattacaca tatcaccggt
      601 gatgacaata gcttttctgg aaacaagcaa gagaatctct cgatattgtc cgtggtggca
      661 gataatttcc ccggttatcg tgaggaagtt aagctttgct ccgttgcggt ctgatgtcag
      721 agacctggta cagggtgggc gctggcggct tgtttgttgg gtagaaagcg gaatgtcttt
      781 tcgatagact tggccacaag ttttgcatct tgagccggag cctgcacctt gaaagcaaag
      841 gcacgcggct tgtcggaggc aatgtctccc agaatgggca gggatggtag gttttcaagg
      901 gcgccaaagg tgactttgct gagaagtccg gagaccgtaa tattgcgact gaggcgtccg
      961 attgtgccgc ctagaaacga ttcggtgacc cgcggtagcg tgcctgctat ttccagatct
     1021 attgtctgct ctgaaagatc ggccgcgccg gcaccccaga gtctcaggtc gtcgcctgag
     1081 aagcgcagtt ctttgatggc cagtgcccct ttgaagatct gaaaacggct ggtaagttca
     1141 ttgaattcac cggatcgcgc cggcgctacc gactgcaaaa ggttgttgat gttgaagccc
     1201 agaataccct gtgagagcaa gttggcctgg gtaagtttgg tttgcaattg accgaatctg
     1261 gccacgattc ccttctggat tacgatattg cccgtacctt ccatgtttga caccatctgc
     1321 ttgtcggtgg tgccctgggt ggtgatatgc aattcgccgt cgagggctcc ggttagttcg
     1381 tccggtgcgt cgaaaagcct gtctacaatc tcttccatgt gaatctgtga gagataaatg
     1441 ttgaccccca gtccattttt ttccgtgtcg aagtgaccgt ccatttttat gtgaccgccg
     1501 gcagttctgg cggtaatgtc tttgagaaga aatttaggcg gcgcaggcaa atcgccattg
     1561 gtaggttcac tcacctgaat ttcgatcagt ccgccgatat cttgtacgcg cagtttccga
     1621 aagcgcattc tgtctatatc tagcctggct acggaaattg aataggggtc atgcttgctg
     1681 gcattgaggt tgtccgtaga gatactgccg ttcaagtcgt agagttgaaa atctttgttg
     1741 ctgactctgt cgagggagat cttgcctgta agcttgggat gtttgatggg gccctccatg
     1801 gatatgaaac catccacggt gccctgcatg ttcttcgtgt ctaaattgtt gtcgaccaga
     1861 cccacgaggg tcttggccgg aaccggattg ggagagagta tgctcaaact gacggtgtca
     1921 tccacaaaag accactgccc ttgagctttc agggctgcct ctcccaggag caggttggta
     1981 ttgtgcagat tgagaccggc tttatctata gtgagggagc catccaggtt cagctcttcg
     2041 tcacgaggtt ggttgatgac gaagtagggc gatgaaatct tcagtcggct atcagccgct
     2101 gcatgggagt tgaagaccat ctggttcttg tccgggttgc cctgaatttg tgctctcacc
     2161 acc
//
LOCUS       DAOQNZ010000709         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32578_length_2162_cov_3.496440, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000709 DAOQNZ010000000
VERSION     DAOQNZ010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32578_length_2162_cov_3.496440"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1844)
                     /gene="amrB"
                     /locus_tag="PKW73_12430"
     CDS             complement(177..1844)
                     /gene="amrB"
                     /locus_tag="PKW73_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNA74142.1"
                     /translation="METVPRAVRSALILWLVVISLGLTACGVDFKEGKLFLFGSTGKR
                     SLRFAGTWYEAEPAKLSRQIESFLAAAGEKESKIPVPQGQVLAVVAPHAGYMFSGKTA
                     AYSYLAASKSAMSGQKIKRVFLLGPSHYKGFHGAALTLDKSFQTVLGELTVDTEVVEE
                     LRQNILFQEATKAHQQEHSLELQLAFIKACFGDVKIVPVLVGQLDDSFEARFIARELR
                     QHLEDGDLVVVSSDFTHFGPRFEYEPFPYESGGRFREKLKALDMEALRYLERNDLEGF
                     MQFYKRTDDTICGVYALCVLLALLPEDSRGYLLDYKTSRDVLAVQDKLDSDSDLDLPA
                     ADDPHSVSYMAVEFRSPESSWNKLRETALAPEPLTKDERKNLLKLARTTLDVFVRTGK
                     TPALAELTGEHGIVLSERLKLPHGVFVTLHKNEKGERSLRGCIGYIFPIKPLYQGVIE
                     NTISAASRDPRFLPVAPSELKNLEVEVSVLTPPHKIARYEDVKIGEDGILLKCRGRQA
                     VFLPSVATEYGWGLEETLKQLSLKAGLSEDAYKGDAKFEVFRSEMIE"
     gene            complement(1786..>2162)
                     /locus_tag="PKW73_12435"
     CDS             complement(1786..>2162)
                     /locus_tag="PKW73_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74143.1"
                     /translation="KGPVKAARPAQGGASQAAIGAYCGRVWAKVAGKWLVPDGNNHVV
                     LTTELASDGSYGDISVTSAPKNQEAEAAAMTALDQSKPLDLLPTGMARGKMTITFDSK
                     ADPHGDCSSGGSVRLDPLAGGN"
BASE COUNT          513 a          604 c          549 g          496 t
ORIGIN      
        1 caagggttct atcgaaaagg aagtggcacc gagcgtggag gcggaattga cccgcttcgc
       61 caaccaggat cataccctgg tctttttgct cttcgccggc ttccaggtga tggtgcaaaa
      121 tctgctggtt tatttcatga gtcttggaac tgctactgtt cctttcatca actagcttat
      181 tcaatcattt cgctgcgaaa gacttcaaac ttagcatcgc ccttgtaggc gtcttcactc
      241 agtccggctt tcaaggacaa ttgcttgagg gtctcctcga gcccccagcc gtactcagtg
      301 gctacggaag gtaaaaagac tgcctgccgg ccgcggcatt tgagcagtat gccgtcttcg
      361 ccaatcttga cgtcctcata gcgagctatc ttgtggggag gcgtcagcac gctaacttcc
      421 acttccagat tcttgagttc ggacggtgcc accggcagga aacgcggatc gcggctggct
      481 gctgaaattg tattctcgat gacgccctga tagaggggtt tgatggggaa aatatagccg
      541 atgcaaccgc gcaagctccg ttcgcctttc tcattcttgt gcaaagtgac gaaaacaccg
      601 tggggcaact tgaggcgctc gctcaaaaca ataccgtgtt ccccggtcaa ttctgccagt
      661 gccggagtct tgccggtacg aacgaaaaca tccagggtag tccgggctag cttgagcaga
      721 tttttgcgct catccttggt gagcggttcc ggcgcgagag cggtttctct cagcttattc
      781 caactgcttt cgggcgaacg aaattctacg gccatgtaag aaacactatg gggatcatca
      841 gcagcgggca aatccaaatc cgaatccgaa tccaatttat cttgcactgc cagtacatcc
      901 cgagatgtct tgtagtcaag caaataacct ctgctgtctt ccggcaaaag agccaggaga
      961 acacaaagag catacacacc gcagatagta tcatcggtgc gtttataaaa ctgcatgaag
     1021 ccttccagat cattgcgctc aagatagcgc aatgcttcca tatcgagagc tttcagtttc
     1081 tctctgaaac gaccgccgct ttcatagggg aaaggttcat actcaaagcg cggaccaaaa
     1141 tgggtaaagt cggatgagac cacgacaaga tcgccgtcct caagatgctg cctcagttct
     1201 ctagctatga agcgagcttc aaaagaatcg tccaattgcc cgaccaagac aggtacgatt
     1261 tttacatcac caaaacaagc cttgataaag gccaattgca attcaaggga atgctcctgt
     1321 tggtgcgcct tggtagcctc ctgaaagagg atattctgcc gcagttcttc caccacctca
     1381 gtatctacgg tcagttcgcc cagcacggtc tgaaagctct tatcgagggt gagagcggca
     1441 ccatggaagc ccttgtaatg agagggaccg agcaaaaata cacgcttgat tttctgcccg
     1501 ctcatggcac tcttgctggc cgccaaataa gaatatgcag ccgtttttcc ggagaacata
     1561 taaccggcat ggggtgccac cacggccagt acctgcccct ggggcaccgg aatcttgctc
     1621 tctttctcac cggcagcggc aaggaagctt tcaatttgcc tggaaagctt ggccggttca
     1681 gcctcatacc aggtaccggc gaagcgtagc gatcttttac cggtggagcc gaacagaaac
     1741 aacttaccct ccttgaagtc caccccgcat gcggtcagac caagactaat taccaccagc
     1801 cagaggatca aggcggaccg aaccgcccga ggaacagtct ccatgtggat ccgccttaga
     1861 atcaaaggta atggtcatct taccccgggc cataccggtg ggcaagaggt ccaggggctt
     1921 ggactgatcg agggcggtca tagccgccgc ttccgcttcc tgattcttgg gagcacttgt
     1981 caccgagatg tctccgtagg aaccgtctga agccagttct gtcgtgagta ccacatgatt
     2041 gttaccgtcc ggtaccagcc atttgcctgc tacctttgcc cagacccggc cgcaataggc
     2101 cccgatcgca gcctgggagg caccgccctg agcagggcga gccgccttga ccggtccctt
     2161 tc
//
LOCUS       DAOQNZ010000710         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32611_length_2161_cov_3.508547, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000710 DAOQNZ010000000
VERSION     DAOQNZ010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32611_length_2161_cov_3.508547"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PKW73_12440"
     CDS             complement(<1..1115)
                     /locus_tag="PKW73_12440"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA74144.1"
                     /translation="MPRVSNKRTYLDPIHQDIVLDWDKPAERLVIDLIDAVEFQRLRR
                     IHQLGVSYFTFQGAEGSRFTHSVGVMHVASRLAEILISRQPEAESERAVILASALLHD
                     VGHGPFSHVTEKILGYDHEDWSCKVVSGDTQIRAILDNYKEEPGLADKIVKVLKKTYS
                     PHYVSHIVSSQLDCDRFDYLLRDSYMTGTAYGLFAMKRILRSMEIDENEDRLLVVGEK
                     GQIAVEDYLFARYSMYAQVYYHRKNLAARAMLGKLIQRAKFVMEQSPGEISFIDEETQ
                     LWLTGQPLTVEQYLRLDDVQFFYHIKRWLKDKDPILQDMAFRFLNRRIFKASRINSRD
                     KAEIAAIEAQARQAVASLGYDPEYYVAVESTGFRPYD"
     gene            1231..>2161
                     /locus_tag="PKW73_12445"
     CDS             1231..>2161
                     /locus_tag="PKW73_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74145.1"
                     /translation="MEPLATGPQSLGDLVGTAFRLVRRNIGLYWKVLLVPAMLSAIGS
                     NLAQYSLKSWVQVAAKSQLAVTPFAIAMSVILVCIVVWFYAAWEMCQRCSAMARLLLG
                     VNNGYQEAYARLKQRGWAVFGAYNLAILPPLLVLMLWAVVAVAAVPLVPHGSTERYVV
                     GALVFGAIGFFLTISIAITSLYGAILMASAAVEDRPFKELLAKSLSFTMARPLRGGSY
                     ICLLTTALALVSVAISSPITLASIIHALMVSRDGVTSAMASAEDPVYLQVVGVGFDVV
                     LNMVTFAVAYTGYVLFYRDLRLRLEGSDLEHRLV"
BASE COUNT          399 a          537 c          639 g          586 t
ORIGIN      
        1 taatcgtagg ggcggaagcc cgttgattct acggctacat agtattccgg gtcgtagccg
       61 agagaggcta cagcctgccg cgcctgggct tcgatggcgg cgatttctgc tttgtcgcgg
      121 ctatttatgc ggctggcttt gaagatgcgg cggttgagaa agcggaaggc catgtcttgc
      181 aggattgggt ctttgtcttt gagccagcgc ttaatatggt agaaaaattg tacgtcgtcg
      241 aggcgcagat attgttctac cgtcaggggc tgaccggtga gccagagctg tgtttcttcg
      301 tcgatgaaag agatttcgcc ggggctttgc tccatgacga attttgctct ttgtatgagc
      361 ttgcctaaca tggctctggc tgccagattt ttgcggtggt agtaaacctg ggcgtacata
      421 gaatagcgtg cgaagaggta gtcttccacg gctatttgcc ctttctcgcc tacaactaga
      481 aggcggtctt cattttcgtc aatttccatt gagcgcaaaa tgcgcttcat ggcgaagagc
      541 ccgtaagcgg tgccggtcat gtagctatcg cgcagcagat agtcgaagcg gtcgcagtca
      601 agctggcttg agactatgtg cgagacataa tggggcgaat aggttttctt caagactttg
      661 acaatcttgt cggccagtcc cggctcttct ttatagttgt cgaggatggc tctaatctgt
      721 gtgtcgccgg agacgacttt gcaagaccag tcttcgtggt catagccgag aattttttcg
      781 gtgacgtggc tgaagggtcc gtgcccgacg tcgtgcagaa gggcggaggc gaggatgacg
      841 gcccgttcgc tctccgcttc cggttgtctt gaaatgagta tttcagccag gcgcgatgcc
      901 acatgcatga cgcccacgga gtgagtgaag cgcgagcctt ccgcaccctg aaaggtgaaa
      961 taggagaccc cgagctgatg aatgcgcctc agcctttgaa actcgacggc atcaatgagg
     1021 tctatgacca gcctttcggc gggtttatcc cagtccagta cgatgtcctg gtggattgga
     1081 tccaggtagg tccgtttgtt tgagactctt ggcatgccgt ttccagtaaa tatagcttgg
     1141 aaatattata ctgacagaag ctctcattct gagcttaaca tcaccaatgg ttgctgcctt
     1201 atcaaattgg aaccgagtgc aagtacaact gtggagccgc tggctaccgg gccgcaatcc
     1261 ctgggagacc tggtcggcac ggcatttcga ctggtacgcc gcaacatagg tctatattgg
     1321 aaggtgctcc tggtgccggc catgctcagc gccatcggct ccaatctggc tcagtacagc
     1381 ttgaagtcct gggtgcaagt ggcggcaaag tcgcaacttg ccgtgacacc ctttgccatt
     1441 gccatgagtg tgattctggt ttgcattgtg gtctggttct acgccgcctg ggagatgtgt
     1501 cagcgctgct cggccatggc caggcttttg cttggtgtca ataacggcta tcaagaggct
     1561 tatgccaggc tgaagcagcg tggttgggct gtcttcggcg cctacaacct ggctattttg
     1621 ccgcccctcc tggtgctcat gctctgggcc gttgttgctg tggcggcggt gcccctggtg
     1681 ccccatggca gtactgagcg ctatgtggtg ggggcccttg ttttcggtgc catcggcttt
     1741 ttcttgacca tctctattgc cattacatct ctttacggag ccatcttgat ggccagcgcc
     1801 gctgtggaag accgaccttt taaagagctt ctggcgaaga gcctgtcttt taccatggct
     1861 agacccttgc ggggtggctc ctatatttgc cttttgacca cggccctggc ccttgtcagt
     1921 gtggctattt ccagcccgat cacgctggct tccattattc acgctctcat ggtgagccgc
     1981 gacggtgtta cttccgccat ggcttcggcg gaagatccgg tctacctgca agttgtgggc
     2041 gtgggttttg atgtggtact aaatatggtg acattcgccg tggcttatac gggctatgtg
     2101 cttttttatc gcgatttgag gttgcgcctg gagggctcgg atctcgagca ccggttggtg
     2161 g
//
LOCUS       DAOQNZ010000711         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32670_length_2159_cov_3.785646, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000711 DAOQNZ010000000
VERSION     DAOQNZ010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32670_length_2159_cov_3.785646"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKW73_12450"
     CDS             complement(<1..214)
                     /locus_tag="PKW73_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74146.1"
                     /translation="MGFESTAFSDSNPRRFTFESSGEALTLTAAALGTAALVLASRGR
                     NLTSLATAGTSEGLLGGAVRSQLKSEA"
     gene            416..643
                     /locus_tag="PKW73_12455"
     CDS             416..643
                     /locus_tag="PKW73_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74147.1"
                     /translation="MNKDVEPVRALNESETIPETPSEALYTALSRNDLNGVIRILREA
                     EPGNWGKILPKKEIATVIGKYKLRLKADETE"
     gene            complement(698..1255)
                     /locus_tag="PKW73_12460"
     CDS             complement(698..1255)
                     /locus_tag="PKW73_12460"
                     /inference="COORDINATES: protein motif:HMM:NF025658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HNA74148.1"
                     /translation="MEQIGRPAHPRSPKVVPICIYIGDAFQYHDPVQSLWLTMVKLVF
                     ADGPEINLNKLSGEACNLYLLELLDREVQSGGLEQFIYNSSGSCFEETKAALVNLGAT
                     TMLALLEEAGKLFPLGMVPKELALRRQVLHDIPATWLAKLDEEFIQSVGCPLDASGNQ
                     IEDLRQIAIAYMKVHAECLVASSTP"
     gene            1449..2024
                     /locus_tag="PKW73_12465"
     CDS             1449..2024
                     /locus_tag="PKW73_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74149.1"
                     /translation="MSPERPPFVVPIGLSAQEYYDLGIRYKLAGWIQQSKKSLRTAML
                     LDPEGVGKRAGTYLKAYLPRNEVPEEAIVENIKAVNLSMQDKKSAEKALLELTKRYPN
                     FEWPFGNLGHQYIEEGRLPEAKESLLKALAINPNYVSALVHMAALNLKENKEQEADNY
                     LAEILKLDPEYKSNVLDNYPALKERLKALAP"
BASE COUNT          606 a          543 c          519 g          491 t
ORIGIN      
        1 tagcctcgct ctttagttgc gacctgaccg caccaccgag aagcccctcg gaggtaccgg
       61 ctgtagccag actggtcaga ttacgaccgc gactggctaa aaccagggcg gcagtgccca
      121 atgcggcagc agtaagagtg agagcctcac cggaagactc aaaagtgaac cggcgcggat
      181 tgctatcgct gaatgctgtg ctttcgaaac ccatagatca tttataagtg gactttccac
      241 cgttgtcact cagtaaatta cgtagccaca agagtgccac aaaggcgtgt tcactgagtt
      301 ccatcgtcgc cgtgcccggc tgtgcggagc tacgtatttc acaccgggtc gaacacaaca
      361 acgacacagg gttgcgcata ttatcagtac agaccccgca ctgagagtag cgcaaatgaa
      421 caaagacgtt gaaccggtgc gggcgctaaa tgagtcggag acaattccag aaactccttc
      481 agaggcactc tacacggcgc tttccagaaa tgatctaaac ggcgtcatca gaatattgag
      541 agaagccgaa ccaggcaact ggggcaaaat acttccgaag aaagaaatag cgacagtcat
      601 cggtaaatac aagctccgat taaaagccga tgaaactgag tgactcttac gacctgccat
      661 aaagagagtc gctcttctca gttatcagca aaagaaccta aggagtactg gatgcaacta
      721 aacattcagc atggactttc atgtaagcga ttgctatttg cctgagatct tcaatctgat
      781 tacctgaagc atcgagaggg caccctacgg actgaatgaa ctcctcatcc aacttggcca
      841 accaagttgc cggaatgtca tgaagaacct gcctgcgcag cgctagctct ttcggcacca
      901 taccaagggg aaacaacttg ccggcttctt ctagcagagc aagcatggtt gtagcaccta
      961 agttaaccag agccgctttc gtttcttcaa agcaactccc tgagctattg taaatgaact
     1021 gctctaagcc gccgctttga acttctctat ctaaaagttc aagcaagtag agattacagg
     1081 cttctccgga aagtttattc agatttattt ctgggccgtc ggcaaataca agctttacca
     1141 ttgtgagcca gagagattga actggatcgt gatattgaaa ggcatccccg atatagatac
     1201 aaatcggcac cactttcggt gaacgggggt gggcgggtct tccaatctgc tccatgagat
     1261 ttcaccaaga acgcgccgaa atgacacgat gattataact tcagactcag gctttgcgct
     1321 gagagtattg cacacttcga gtatccttta tttccaggta atactctgcg atacctgacg
     1381 agttgaagga gattggcagt agtatctaaa attgctcctg tagaaactcc tttcagaaag
     1441 gtccgtttat gtctccagaa cgtcctccat ttgtagttcc aattggtctg agtgcccagg
     1501 aatactatga tctcggcata agatacaaac tggctggctg gattcaacag tcaaagaagt
     1561 ccctgcgcac ggcaatgctg ctcgatcccg aaggcgtcgg caaaagagca gggacttatt
     1621 taaaagccta tcttcctaga aacgaggtgc cggaagaagc catagttgaa aacatcaagg
     1681 ccgtcaattt gagcatgcag gacaagaagt ccgcagagaa agccctattg gaactgacca
     1741 aaaggtatcc gaatttcgag tggccttttg gcaacctggg gcatcaatat atcgaagaag
     1801 ggcgcctgcc ggaagcgaag gaatcactat tgaaggctct cgccatcaac cctaactacg
     1861 ttagcgccct ggtgcacatg gccgccttga atctgaaaga gaacaaagag caagaggcag
     1921 acaattatct ggctgagatc ctcaagcttg acccggagta taagtccaac gtgctggaca
     1981 actatcccgc attgaaagag cggctgaagg cacttgctcc ttagccgctc ttagctggat
     2041 tttctagctc tcgacttcta ttcccacata ggaatgcgta cgccggattt cttggcgaaa
     2101 tctatggctt cctcataacc ggcgtcaacg tgcctgatca cacccatgcc ggggtcggt
//
LOCUS       DAOQNZ010000712         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32733_length_2157_cov_3.458135, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000712 DAOQNZ010000000
VERSION     DAOQNZ010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32733_length_2157_cov_3.458135"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PKW73_12470"
     CDS             <1..806
                     /locus_tag="PKW73_12470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74150.1"
                     /translation="ALGAGGMSTVYKAKHRFLDKYVAIKLLNAHLSSDGKAVQRFQLE
                     AKAAYDLKHPNLISIYDFGISPDGQAYIVMDYLEGESLSQRIERQGKLSLMEALPIFA
                     DVAAGLAHAHQNNVLHRDIKPSNVMLVTGEDGSGTARIVDFGLAKNYDENAVKLTQTG
                     EIFGSPLYMSPEQCRGAVLDQRSDIYSFGCLMYETLTGFAPISGANAYETFRLKFSEV
                     PPPFASTLNIPTWLSSLIMATLRVEPDERPTTASEIREALASYVRKRGQ"
     gene            complement(823..1857)
                     /locus_tag="PKW73_12475"
     CDS             complement(823..1857)
                     /locus_tag="PKW73_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74151.1"
                     /translation="MGLGSGKFQLSVGWRQANATKSYNDSTYNKNFTDLWGPRMRQSV
                     LDVTGRYYVNRRLSVLATLPVTMNRFSLLIPPKGPLQGVREGWSVGGIGDLQLHSQAT
                     VLDPRDHPYENMIVGLGIKIPTGDWDEQRTIPNLNGNQWAKRAVYPAAVMPGDGGTGI
                     VGTFDAFKTFRAPRILRGQSVFVSGSYLSNPRNTNGTSSIINSAGVPLTPNFLNELTN
                     SVADAYAVQAGVSLKIPGTWNKPSLKGMRARVVGRWEGVRSHDLIGPSGGYRQPGYAM
                     SIGPGLTWAREKDMIMVDVPLTFLRYINAERSAVPGPSNGNTPAAFNESRNLGLVAPV
                     SVSVRYIRSF"
BASE COUNT          472 a          569 c          597 g          519 t
ORIGIN      
        1 ccgctcttgg tgccggtggt atgagtaccg tgtataaagc caagcacaga ttcctggata
       61 aatacgtagc cattaaactt ctcaatgccc atctgtcctc cgatggcaag gccgttcaac
      121 gctttcagtt ggaggctaag gcggcttacg atctcaaaca tcctaatttg atatcaatat
      181 acgacttcgg tatttctccc gatggtcagg cttatatcgt catggattat ctggaaggcg
      241 agagcctttc tcagcgtatc gagcggcagg gcaaactgag tttgatggaa gcccttccta
      301 tttttgccga tgtggctgcc ggtcttgccc atgcccatca gaataatgtt ctgcaccgtg
      361 atattaagcc ttctaatgtc atgttggtga ccggagaaga cggcagtggc accgctagaa
      421 ttgttgactt tggtctggcc aagaactatg acgagaacgc cgtcaaactg acacagaccg
      481 gcgaaatatt cggcagtccg ctctatatga gccccgagca gtgtcgcggt gccgtccttg
      541 atcagcgctc cgatatttat tccttcggct gcctcatgta cgaaaccctt actggctttg
      601 cccctatcag cggtgccaat gcctatgaga catttcgcct caagttttcg gaagtgccgc
      661 cgcctttcgc ttctactctc aatattccaa cctggctttc aagcttgata atggctactc
      721 tgcgggtaga gccggatgag cggccgacta ctgcttccga gattcgcgag gcgcttgctt
      781 cgtatgtgcg caagcgcggt cagtaagggc cggtaaacct tcttagaagc tgcgaatata
      841 gcgcactgac acgctgaccg gtgccaccag accaagattg cgactttcat tgaaagcggc
      901 tggcgtgttg ccgttactgg gaccaggtac ggctgagcgc tcggcgttga tatagcgtaa
      961 gaaggtgagc ggcacatcta ccatgatcat gtctttctct ctggcccagg tcagtccggg
     1021 accgatagac atggcataac cgggctggcg atagccgccg gaaggaccaa tcaggtcatg
     1081 actgcgtact ccttcccagc gccccaccac cctggctcgc atgcccttga ggctgggttt
     1141 gttccaggtg ccgggaattt tcaaggagac tcccgcttgc actgcatagg catcggctac
     1201 ggagttagtc agctcattga ggaaattggg tgtgagcggc acgcctgccg aatttatgat
     1261 ggatgatgta ccgttggtat tgcgcgggtt cgacaaatag gagcctgaga cgaaaacaga
     1321 ttgaccgcgg agaattcgcg gtgcgcgaaa ggtcttgaag gcatcgaagg ttccgacgat
     1381 accggtgccg ccgtcgccgg gcatgacggc tgctgggtag acggcgcgct ttgcccattg
     1441 attgccgttc aaattgggga tggttctctg ttcatcccag tcgccggtgg gtattttgat
     1501 accgagtccg acaatcatat tttcgtaagg gtggtctctg gggtccaata ccgtcgcctg
     1561 gctgtgcagt tgcaggtcgc ctatgccgcc cacgctccag ccttcgcgca cgccctgcag
     1621 gggacctttc ggtggaatca aaagcgagaa gcgattcatg gttacaggaa gagtggccag
     1681 cacgctgagc cgccgattga cgtagtagcg accggtgaca tccagcacgg attgccgcat
     1741 ccgtggtccc catagatccg tgaaattctt attgtaggtg ctgtcgttgt aagatttggt
     1801 ggcattggct tgccgccagc ctacagacag ttgaaactta cctgacccaa gtcccatacc
     1861 tgggaccatc cctcttgcgc tggctcagcc ctggccccag gcggctttct cacctaaaag
     1921 cagaaagagg tagagaaagc agagcctgag aaaaaccatt tgagggattg tgaaatgtct
     1981 gaaagcggac tcgcttaagg caagcctgaa aggagctgag aggttgggat ttaccctgct
     2041 caatacttct gtaccgtgct atctatctcg tcggcccagg ctaagatgcc gccctgcaaa
     2101 ttgagagcgg aatcgtaccc gtttgctctc aaaaattgta cacagcgcat ggagcgg
//
LOCUS       DAOQNZ010000713         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32750_length_2156_cov_4.649691, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000713 DAOQNZ010000000
VERSION     DAOQNZ010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32750_length_2156_cov_4.649691"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKW73_12480"
     CDS             complement(<1..377)
                     /locus_tag="PKW73_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74152.1"
                     /translation="MVMLNFLQSLSPSGKAVFAASSRRRTGLSLISAILAPVFLCTLT
                     FPLGLAASAYSGNDPLLEKANRSYRSGRYQEAGGLLNRVLAGSPSPETSAGAHNLLGK
                     VYHRLGKQDEALKQYQQALKDEPG"
     gene            469..2004
                     /locus_tag="PKW73_12485"
     CDS             469..2004
                     /locus_tag="PKW73_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74153.1"
                     /translation="MRRAIQTGLILSLVLLTGFSLSPADGRSKAAAKAETDAPDTGAE
                     DPGCKECVKNAATLFARGDNEGALASLRAWQAKCTRNLQLHLLLNTVLLRTKNGQQEA
                     LKVARQACEIDPNSSLAHLQTAMTLLMVGQASEAVPEFEKVVSLDPTSYEAWQALAEA
                     YGASGEREKEAEAKRKAGSLNPTAQASRTRLISSLDKAGNAGQARAEYKKLCQDEQLP
                     GELFLSLGRDAITMGYFAEGLACLERFSANQKDGPALSEARWLKTVASYLEGSEGSQQ
                     FSKSIDNLKGGQKYSIRAIACSGLKELDNGNYPEAQKLISEAGSKAPTDPAVLLAMGK
                     LALAEGKYKECIEKMEDAIKAEPALAGARIFIAKASFKDGDLIEAMSQARECARVKDL
                     APRAQALELRAKLRDDGASREGLKELAAKVDGHYRQNPQDPELNVAVAYLDLNSKDWN
                     GAREKFEKALTAYPACEDAMIGMARLAKQEANLGLAKELLTKAISIAPGDSEAKDLSN
                     TLK"
BASE COUNT          581 a          583 c          615 g          377 t
ORIGIN      
        1 cccggctcgt ctttcaaagc ctgctgatat tgcttgagtg cctcatcttg cttaccaagc
       61 cggtggtaaa ccttgcccag taaattgtgc gcaccggcag aagtctccgg tgaggggctg
      121 ccggccagaa cacggttcag caagccgccc gcctcctgat agcgccccga ccggtaagag
      181 cggttggcct tctctagaag cgggtcattg cccgagtaag cggaggcggc aagccccagc
      241 gggaaagtca gggtacataa gaagaccggg gcaagaatag ccgatatgag gctcaggccg
      301 gtcctgcgcc gactacttgc ggcaaacact gccttgccgg atggagataa cgattgcagg
      361 aaattgagca taaccatatt cagggttgcg ggaagttgag catatgatgc atcagtataa
      421 tgctaagtaa cttaaggcta aatggcaaag aagcaaagga gcaagctttt gagacgcgct
      481 atccaaaccg ggctaatctt gagcctcgtc cttctaacgg gcttctccct ctctcctgcc
      541 gatggccgtt caaaggcggc ggcgaaagca gaaacggacg cccctgacac cggcgcggaa
      601 gaccccggct gcaaagagtg cgtcaagaac gccgccaccc tgtttgcacg cggcgacaac
      661 gaaggcgccc tggcttcgct ccgggcctgg caggctaagt gcaccaggaa cctgcaactg
      721 catttgctgc tcaacactgt ccttttgcgc acaaaaaacg ggcagcaaga agccttgaag
      781 gttgccaggc aagcctgtga gatagacccg aattccagcc tggcccatct acagacagcc
      841 atgaccttgc tcatggtcgg gcaagcatcg gaggcggtgc ccgaattcga gaaagtggta
      901 agcctcgatc ccaccagcta tgaagcctgg caagccctgg ctgaagccta tggcgccagc
      961 ggcgaaagag agaaggaggc ggaagccaaa agaaaagccg gcagtctcaa tccgactgcc
     1021 caagccagtc gcacccgact gatctcctcc cttgacaaag caggcaatgc cggtcaagcc
     1081 cgggcagaat acaagaagct ctgtcaggac gagcaactgc ctggggagtt gtttctaagt
     1141 ctgggtcgag atgccatcac catgggctat tttgccgaag gactggcttg cctggagcgc
     1201 ttttccgcca accagaagga tggtccggcc ctttccgaag cacggtggtt aaagacggta
     1261 gcctcatatc tggaaggcag tgaaggcagt cagcaatttt cgaaaagtat cgacaatttg
     1321 aaaggtggtc agaaatattc catccgcgcc atagcctgca gcggtctgaa ggaattggac
     1381 aacggcaatt acccggaagc acaaaagcta atatcggagg ccggcagcaa agctcccacc
     1441 gacccggcag tgctcctggc tatggggaaa ttagccctgg cggaaggcaa atacaaagag
     1501 tgcattgaaa agatggaaga cgccattaag gcagaaccgg cactggccgg ggcaagaatc
     1561 ttcatcgcca aagccagttt taaagacggc gatcttatcg aagccatgag ccaggccagg
     1621 gaatgcgccc gcgtcaaaga cctggccccc agggcacagg ccctggaact acgggccaaa
     1681 ttaagagacg acggtgccag tagagagggg ctgaaagagt tggccgcaaa agtcgacggc
     1741 cattaccggc aaaatccgca agaccccgag ttgaacgtag cggtcgctta tcttgacttg
     1801 aacagtaaag actggaacgg cgcccgcgaa aagttcgaga aggcactgac ggcctaccca
     1861 gcctgcgaag atgccatgat tggaatggct cgcctggcca agcaggaagc aaatctgggt
     1921 ctggcaaaag agttgttgac aaaggcaatt tccatagcac cgggcgacag tgaagcgaaa
     1981 gacctcagta atacgctaaa atagcccgag caaatatggt tacaagtcac aaagcaattc
     2041 ttgaatcgat caacccctcc accaaagaag tgctggggcg ggtaccgatc atgtcgaaag
     2101 tccaggtgga cgagattgtg gcagccagcc acaaagccta tgaaatctgg caactc
//
LOCUS       DAOQNZ010000714         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32830_length_2154_cov_2.565507, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000714 DAOQNZ010000000
VERSION     DAOQNZ010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32830_length_2154_cov_2.565507"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="PKW73_12490"
     CDS             complement(<1..2108)
                     /locus_tag="PKW73_12490"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNA74154.1"
                     /translation="MNGLTLGLSASWLGIWIHAVFFKSGGPSCKFGWMLLLSTSPFLL
                     FAAFCLSLDLWQKTNASMRRITGTFSIMGIVLSMLFVLAPVGRVSYIQSLITAARHSS
                     GDDLAQAVGALRTSASDEDLRPSKSTVSGFALAEMLVPNRGLEDGSDIDKDLFFRITG
                     KPYVSEPGGLPWQSHPELWNPLIGMKVPGLSLSKSQMSGLIDSETLSASVDWTMTFHN
                     SSAASQEARGEITVPEGAAVSRVTLWVNGQPQEGAFAAKSQAQEAYQSTVEQRRDPLL
                     VTMNSPGRILVQCFPVPANGGEMRIRIGFKVPLATSDGKTCTMDLPRMADGNFVQLKR
                     HRVSFSSDRKVVGQASAAGLSSAVGEHGYALSGILRTSELGKRMPRLSVVRNSAFKNV
                     AVQDWYSRKPGYIVETLREVKISTPSRLFVVLDTSASLKTQASQIRQALAGLPASLKP
                     MVYLVKDDFTAAVVNTSNDAFAPAPVPSTTDVNETSSAPVQPVSLKQAEEMVTEELFA
                     GGKDNIAGLREVLETASERAGSAVLWIHGPQPQSRKMSESVLEMINRVKLYDLEVVAG
                     ANNVLPTIQSDDVSHLVSCETVKSGLSSSQLKDLLAGMKNGKTATVIERTRTEGKPAI
                     PVIADADAAARLTCLWASDEVTRRLESGPYKAAQSLAVQYRLVTPASGAVVLENARAY
                     AAKGLKPGAYVDAPDNSQPPA"
BASE COUNT          539 a          614 c          557 g          444 t
ORIGIN      
        1 gccggtggct gagagttatc gggggcatcg acataggcac ctggtttcaa accctttgcg
       61 gcataggccc tggcattttc gagcaccacg gcaccactgg cgggtgtgac caggcgatac
      121 tgcacagcca gagactgagc cgctttatac ggtcccgact caagacggcg agttacttcg
      181 tcactagccc acaggcatgt aagccttgct gccgcatcgg cgtcggcaat cactggtatt
      241 gccggtttgc cttccgttct ggttctttcg atgaccgtcg ctgtcttacc gttcttcata
      301 ccggcaagca aatccttgag ttgcgaagaa gaaagcccgc tcttgactgt ttcgcaacta
      361 accagatggg agacatcatc gctctgaatg gtaggcaaaa cattattggc tcctgccacc
      421 acttccagat cgtacaactt cacccggttg atcatctcta agaccgattc ggacatctta
      481 cggctttgag gctgtggacc atgaatccag agaacagcac taccggctct ttcggaagcg
      541 gtttccagaa cctcgcgcag gccggctatg ttgtcctttc caccagcgaa cagttcttca
      601 gttaccattt cctcagcctg cttaagtgaa acaggctgca ccggcgcgct acttgtctca
      661 ttcacgtcag tggtggacgg caccggagcc ggggcaaagg catcgttcga agtatttacc
      721 accgcggcgg tgaaatcatc cttaaccagg taaaccatcg gtttgagaga ggccggcaga
      781 ccagccagag cctgcctgat ctgggaagcc tgcgtcttca gagaggctga tgtatccagc
      841 accacgaaaa gacggctggg cgtgctgatc ttcacttccc tgagggtttc cactatgtag
      901 ccaggcttgc gactatacca gtcttgcacg gcaacattct tgaaagcact gtttctaaca
      961 acggacaacc gaggcattct cttacccaat tcactggttc taagaatgcc gctcaaagca
     1021 tacccgtgct cgccgacagc agaagacaaa ccggcagcgg aagcctgtcc gaccaccttc
     1081 cggtcagaag agaaactaac tcggtggcgc ttcagctgaa cgaaattacc gtctgccatg
     1141 cgcggcaaat ccatcgtgca ggtcttgccg tcggaagtgg caagcggcac cttaaagcct
     1201 atgcggatgc gcatttcacc gccattggca ggcaccggaa agcattgcac gagaatgcga
     1261 ccgggactat tcatggtgac aagcaaagga tctcgccgct gctccacggt cgattgataa
     1321 gcctcttgcg cttgagactt agcggcaaag gccccttctt gcggctgtcc gttcacccag
     1381 agcgttactc ttgacacggc agcaccttcc ggaacagtta tttcgccacg ggcctcctgg
     1441 gaggctgccg aagagttatg aaaagtcatg gtccagtcga cagaggcact taaggtctcg
     1501 gagtcaatca gaccggacat ctgagactta gacagggaca agccaggaac cttcatgccg
     1561 atgagcggat tccacagctc aggatgactc tgccagggca ggcctccggg ctcggaaaca
     1621 tagggctttc cggtaatccg aaagaatagg tctttgtcta tgtcgctacc gtcctcaaga
     1681 ccgcgattgg gcaccagcat ttcggccaga gcaaaaccac tgacagtgct cttggaagga
     1741 cgcaaatcct catcggaagc agatgtacgt aaagcaccga cggcttgcgc caaatcatcg
     1801 ccggagctat gcctggccgc cgtaatgagg gactgaatat agctgacgcg accaacggga
     1861 gccagaacaa acaacatgga cagcactatg cccattatcg aaaatgtgcc tgtaatgcgg
     1921 cgcatactgg catttgtctt ctgccagagg tccagactca aacaaaaggc ggcaaacagc
     1981 aaaaaggggg aagtggaaag caagagcatc cagccaaatt tacaagaagg accgccgctc
     2041 ttgaaaaaca ctgcgtgaat ccagattccc aaccaggaag cagaaagccc cagagtcaaa
     2101 ccgttcatca aaccaatcag ccgaggtcta gccagatagc ctttcacacc gccg
//
LOCUS       DAOQNZ010000715         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32841_length_2153_cov_4.542421, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000715 DAOQNZ010000000
VERSION     DAOQNZ010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32841_length_2153_cov_4.542421"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            457..1980
                     /locus_tag="PKW73_12495"
     CDS             457..1980
                     /locus_tag="PKW73_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNA74155.1"
                     /translation="MNWLGLFSDFDGSAGGRPLYRRIVDHLAALIQGGTLPGGTRLPT
                     NRELAALLQLDRSTIARAYEELISMGLIYSHVGRGSFVCDAPQPDRTDAWRDRYSNYG
                     GELKQLMECLPDPSVSSYPGAVISFAAGIPSADSYPEEEFAAIIKTLADGNAGALFKF
                     SPAGGEPTLRKLVLEHLAGRSLKASDGELMILSGSQQGIDLMSNMFLDAGDLVVTEDP
                     TYFWAISNFRAHRARIVGIPLDEEGIRLDLLQAVLQKGRVKFIYLMPSGQNPTGLTMS
                     LERRRAVLELARRYNTPIFEDDFSGDLDYEGGPLPPLRALPGGQSLVVYQGTFSKALA
                     PGIRLGWLVAPPLVVERLNFAKRGSDLSTSSLTQTLLSEFLKRGSYGRHLERINALYA
                     ARRDTMLGALERCFGESGVKFLRPRGGLFIWMTLPEGMDARTFLSFSEQEGVSFSPGD
                     ICYVGKADPCTLRLSFIQNDTTAIEEGVERLFAAFRKYRRAMGVQTIDRTEPEHVLI"
     gene            2009..>2153
                     /locus_tag="PKW73_12500"
     CDS             2009..>2153
                     /locus_tag="PKW73_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74156.1"
                     /translation="MTRVTKTVETIGKIATGTELVKRSLPAMLKGGVIMDVVNAEQAK
                     IAEE"
BASE COUNT          443 a          570 c          586 g          554 t
ORIGIN      
        1 agtattgaaa ttgctgtcag ggacggcttg ggtagttctt ctgcggaatc gctgcaaatg
       61 taacaatagt gatgcgaaga aatattgcat cagcttttcg ggactttttc gtaaatgaaa
      121 gttggtctgg atgggcgttt tcactgtcaa acagatttta tttttctatt tacctgagtt
      181 tagcgatttt ttgaccaggc tctcgaaaga tagggaatca tggttccggc aagccttcga
      241 aaaaatacag tctcaagtta gccgactgcc aatgcgagaa aaattttaga ttggcttgct
      301 tgtctggagc gattgatcta attggctgga tggttgtatt attgtccgcc tgttgtggga
      361 attggttggg atggctctaa gcgggctcgt ttccaatcca ggcttgcttc tgtggcttgc
      421 cttgggtggg cttgatctaa attggctggt atctgattga attggcttgg cctattttca
      481 gactttgacg gtagtgccgg tggcagaccc ctttaccggc gcatagtcga tcacctggct
      541 gctctcattc aaggcggtac cctgccggga ggcacccgct tgcccaccaa ccgtgagctt
      601 gccgcccttt tgcaactgga ccgctccacc atcgccagag cctatgaaga gcttatttcc
      661 atgggcctca tctattccca cgtgggtcgg ggcagctttg tctgcgatgc tccccagcca
      721 gaccggactg atgcctggag ggaccgctat tccaattatg gtggtgaact caagcaatta
      781 atggaatgcc tgccggaccc atcggtgagt agctatcccg gcgcagtgat ctcctttgcc
      841 gccggcatac cctcagccga ttcctatcct gaggaagagt tcgcggccat tatcaaaact
      901 ctggccgacg gcaatgccgg ggcgctcttc aagttttccc cggccggcgg cgagcccacc
      961 ctgcgcaaac tggtgctgga gcatctggcc ggccgttctc tcaaggccag cgatggggag
     1021 ctcatgattt tgagcggttc ccagcagggc atcgacctca tgtccaacat gtttcttgat
     1081 gccggagatc tggtggtgac ggaagacccc acatatttct gggcaatttc caatttccgg
     1141 gcgcaccggg cccgcatcgt cggcataccc ttagatgaag aaggcattcg tctggatctg
     1201 ctccaggcgg ttctgcaaaa ggggcgagtc aagttcattt acctgatgcc ttccggtcag
     1261 aaccccacag gtctgactat gtccttggaa aggcgccggg ccgtattgga actggccagg
     1321 cgctacaaca ctcccatttt tgaagatgat ttttccggag atcttgatta cgaaggcggt
     1381 cccttgccgc ccctgagagc tcttcccggc ggccagtctc tggtggtcta ccagggcact
     1441 ttctccaaag ccctggctcc cggcatccgt ctgggctggt tggttgctcc gccactagta
     1501 gttgagcgtc tcaacttcgc caagcgcggc agtgatcttt ccaccagttc cctcacccag
     1561 actctattgt ccgagtttct caagcgcggc tcttatggac gccatctgga gcgcattaat
     1621 gctctatatg ccgctcgtcg tgataccatg ctcggtgctc tagagcggtg cttcggcgaa
     1681 agcggcgtca agtttctcag accgcggggc gggctcttca tctggatgac tttgcccgag
     1741 ggcatggatg ctcgtacctt cctcagtttc agcgagcaag aaggggtttc cttttcgccc
     1801 ggcgacatct gttatgtggg taaggccgac ccctgtacct tgagactttc ctttattcaa
     1861 aatgacacta ctgccattga agaaggcgtc gaacgtctgt ttgccgcttt ccgaaaatac
     1921 cgcagagcca tgggcgtaca gactatagat agaaccgaac ctgagcatgt cttgatctag
     1981 gcgtgccacc aaaaaagagg agcttgaaat gacacgggtt actaaaacag tagagacgat
     2041 tggcaagatt gctaccggca ctgaactggt gaagcgttct ctgcctgcca tgctcaaggg
     2101 cggcgtcatc atggacgtgg tcaatgccga acaggccaag attgccgaag agg
//
LOCUS       DAOQNZ010000716         2151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_32905_length_2151_cov_3.555821, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000716 DAOQNZ010000000
VERSION     DAOQNZ010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32905_length_2151_cov_3.555821"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="PKW73_12505"
     CDS             <1..1344
                     /locus_tag="PKW73_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA74157.1"
                     /translation="SSLVMPVLELLRPIPPIAWIPVAMTVFGLGDPSADFVIFIGSFY
                     PILTNTLLGVTSVASDHLDAAKLLGASSWRILKQIIWPSSLPSIFAGLRVGLGFAWMC
                     VVAAEMVAASSGLGYEIQLNRQLLRMDRVVAGMMAIGLIGYMMNRAMQYLERACLPWV
                     HLKQISDESLEESLPVKEAPTAGGQKQAGGALVQVKNLHFGYNQERPQIVDFNIKIMP
                     GEVLCILGGSGCGKTTILRLLAGLLLPQQGGIAITGGVVTEAPVADDPAPNHKAKLTM
                     VFQGSALFPWQTVLENVCFALQSKGVPSDQQKELATAALARVGLSHKATQFPAALSGG
                     QKQRVALARALAYAPDLLLLDEPFAALDSQTRESLQDDLSRLLAEQNVTAVLVTHDIR
                     EAIFLSDRVIMLGKSGATILDQVAVEEPRPRSSEYRYTREFSEMRTRLWKSLHEN"
     gene            1334..>2151
                     /locus_tag="PKW73_12510"
     CDS             1334..>2151
                     /locus_tag="PKW73_12510"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNA74158.1"
                     /translation="MKISSLASTLLTGALIAGLAGCAMVEKTDVAAVETAPTKVVVGW
                     MTSWATAGQIMQAMIHTNICDLYEFKPEFHSFLFGPDINEAALAGKVDVQNSGLVPTI
                     SLLAASEDWIVIGRLIDSPLSIVARQGSGIKTLADLKGKTIAVPFGGGSHPYVLQRLE
                     ATGLTCGKEKGDVKLINLKPSEQAIAITQGSVEAVGTWEPQTALILSKKMGYVIDSER
                     HLGVISIRKQIAKAHPELVEKLLREYIEANYYVALHGRQTDQWFIKAAGFPEALL"
BASE COUNT          496 a          532 c          603 g          520 t
ORIGIN      
        1 agcagtctgg tcatgcccgt cctggaactg ttgagaccga ttccaccgat tgcctggatt
       61 ccggtggcga tgactgtctt cggtttgggc gatccttccg ctgactttgt gatctttatc
      121 ggcagcttct atccgatatt aaccaacacc cttctaggcg ttaccagtgt tgcttccgat
      181 catctagatg cggccaaact cctgggtgct tcctcctgga gaatcttaaa acaaataatc
      241 tggccatcca gcttacccag catctttgcc ggtttacggg tgggtcttgg ctttgcctgg
      301 atgtgcgtgg tggctgccga aatggtggcg gccagttcag ggctgggtta cgagattcag
      361 ctaaatcggc agcttctgcg gatggaccga gtggtagcag ggatgatggc gatcggtctt
      421 ataggttaca tgatgaatcg tgccatgcaa tacctggagc gggcctgtct gccctgggtg
      481 cacctcaagc agattagcga tgaaagtctg gaagagagtc tgcctgtcaa ggaagccccg
      541 actgccggcg ggcagaagca agcgggcggt gctctcgtgc aagttaagaa tctgcacttt
      601 ggatacaacc aggaaagacc gcagatagtg gacttcaata tcaagattat gcctggtgaa
      661 gtgctctgca ttttaggtgg cagtggttgc gggaaaacca caatcttgcg tcttctagcc
      721 gggctgttgc tgccacagca aggcggcatt gccattactg gtggtgtcgt aaccgaggcg
      781 ccggttgctg atgacccggc gcccaatcac aaggcaaaat tgaccatggt ctttcaaggc
      841 tctgcgcttt tcccctggca gacagtactt gagaacgtgt gctttgctct gcaatcaaag
      901 ggggtgcctt ccgaccagca gaaagagttg gctaccgctg ctctcgctag agtgggactg
      961 tcccacaaag ccacgcaatt tccggctgca ctgtccggtg gtcaaaaaca aagagtggct
     1021 ctggcaagag cgctagccta tgctcctgat ttgttgctgc tcgatgaacc ttttgccgct
     1081 cttgatagtc agaccaggga gtccttgcag gacgatttat caaggctgct ggctgaacaa
     1141 aatgttacgg ccgtgcttgt cactcacgat ataagagagg ctatctttct atccgaccgc
     1201 gttataatgc tcggcaaaag tggtgctacc atactcgacc aggtggctgt agaagagccc
     1261 agaccaaggt cttccgagta tcggtatacc agagagttca gcgagatgag aacgagattg
     1321 tggaagagtc ttcatgaaaa ttagttccct ggcatctacc ttactgacag gtgctcttat
     1381 tgccggattg gccggctgtg caatggtgga aaagaccgat gtggctgcgg ttgagacagc
     1441 gccgacaaaa gtagtggtgg gttggatgac ctcgtgggcc acggccggac agataatgca
     1501 ggctatgatc cacaccaata tttgtgacct gtacgaattt aagcctgagt ttcacagttt
     1561 tctctttggc ccagacatca acgaagcggc tctggccggc aaagtagatg tgcaaaacag
     1621 cggtcttgtg ccgactatca gtttgctcgc cgccagtgaa gactggatag tcataggcag
     1681 attgattgac agtccccttt cgatagtggc cagacaaggt tcaggcataa agacccttgc
     1741 tgatctcaaa ggcaaaacca tcgccgtgcc gttcggcggc ggttcccatc cctatgtgtt
     1801 acagcgcctg gaagctacgg ggctgacttg cggcaaagag aaaggcgatg tcaaactcat
     1861 caatctcaaa cccagtgaac aagccatagc catcactcag ggctctgtag aggcagtggg
     1921 cacctgggag ccgcaaacgg ctctcattct ctctaagaag atgggttacg taattgattc
     1981 cgagcgacat ctaggtgtga tcagcattcg caagcaaatc gctaaggcgc accctgagtt
     2041 ggtcgagaag ctgcttcgtg agtacataga ggcaaattat tatgtggcgc ttcatggccg
     2101 gcagaccgat cagtggttta tcaaagccgc aggctttccc gaggctttgc t
//
LOCUS       DAOQNZ010000717         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33100_length_2144_cov_3.512685, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000717 DAOQNZ010000000
VERSION     DAOQNZ010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33100_length_2144_cov_3.512685"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..869)
                     /locus_tag="PKW73_12515"
     CDS             complement(189..869)
                     /locus_tag="PKW73_12515"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNA74159.1"
                     /translation="MIKLIRGLHHFQSSVFLSHKTLFERLAQGQTPDALFITCSDSRI
                     SPSLLTQTKPGDLFILRNAGNIIPPHPACGCEVCGGEAATIEFAVVALGVRDIIVCGH
                     SHCGAVKGLMNPASLSALPATSAWLKHAEAVRRNVLENYPGLDEAQALNVAIQENVLA
                     QLENLRSHPCVAERLAAGTLNLHGWLYHFETGRVFVYQPDEGQFLPLSDAGLEVLPGL
                     VFNQDSSI"
     gene            complement(1409..1750)
                     /locus_tag="PKW73_12520"
     CDS             complement(1409..1750)
                     /locus_tag="PKW73_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74160.1"
                     /translation="MEASLFSWLSAFPGASIEKLRRINQASTSSRHIILAITYGLTDR
                     HLASVNKLIIGLMVARHKAGRMHDQRLEERIEVNLKALHRAINALVGPPPHGADEAAC
                     RSVAGRSRLER"
BASE COUNT          575 a          600 c          522 g          447 t
ORIGIN      
        1 cgcctctcga tactttaagt cctgtcactt tccctgcgta tggggtattc aggttttagc
       61 ggtttattac tacttatagt atctcaaggg ctgaaaaaac aaaaacggaa gcaagcgcga
      121 tttccctacc gcgctcacac aaaattactg gtcagccgga cagcggcaaa ctagtttaaa
      181 tcgacattct aaatcgagct gtcctggttg aaaacgagac ccggcaagac ttccaacccg
      241 gcatccgaga gtggcagaaa ctgcccctca tccggctggt agacaaagac tctgccggtc
      301 tcaaagtggt agagccagcc gtgcaggttc aacgtgccgg cagccaggcg ctccgccaca
      361 cagggatggc tgcgcaggtt ctccaactgc gccagaacgt tctcttgaat ggctacattg
      421 agtgcctgtg cttcatcaag accgggataa ttctccagca cattgcgacg cactgcctcg
      481 gcatgtttga gccaggcact agtggcgggc agagcactga gcgaagccgg gttcataagc
      541 cctttaacgg ctccgcagtg agagtgcccg caaacaatga tatccctcac tcccagagcc
      601 accacggcaa attcaatggt ggcagcctca ccgccgcaaa cttcacaacc gcaggccgga
      661 tggggaggaa taatattacc ggcgttacgc agaatgaaaa ggtctccagg ttttgtctgg
      721 gtcaacaaac tggggctgat gcgggagtcg gagcaggtga taaatagcgc atcaggagtc
      781 tgaccttgag ccaacctttc gaagagtgtc ttatgagata ggaaaaccga tgattgaaag
      841 tgatgaagcc ctctaatgag ctttatcatg gacgatcatc tcggcgcgct gcatttctgg
      901 aaggggattg aaaacgagat ctgcgcctcc gacctcggtt ttcgatactg gattcaatca
      961 gagcggcgat gagaaagtaa ccgaagaaga ctaacaaaag gacgacggga aacgcctcag
     1021 ccgcactttt agcagcgaat aaatgctcca ttgagcacct ccaggttgag taaaaagttg
     1081 agtttgtcct tcttccgacc cgccaataaa gaaagccgga ccatgccgcc tgctcatgat
     1141 tgaaagagca gcaaggcaaa aggcagtgat agcggaatta ctcttgagat aaacgattgc
     1201 ggtaaaggga agttagaaag gacatttaca actagccgaa acaacacggc tgctgcaagg
     1261 cgcgccgccg ccgccagtcc cgacttcggc cggttttggg caatagtcgg tgcttccggc
     1321 agcaactgag aggcttctaa gttaatcgcc ctgtctcagg ctacaagtgt ccggagcagc
     1381 gtcccaaaag cgtcccatcg ggaagtaact accgctcaag cctggagcgc cctgccaccg
     1441 aacgacaggc ggcttcatcg gcaccatgag gaggcggtcc caccagggca tttatggccc
     1501 tatgcaaagc cttcaaatta acctcgatgc gctcttccaa ccgctgatca tgcatacgac
     1561 cggccttgtg gcgagccacc atcaaaccaa taatcaactt gttcacagaa gctagatgcc
     1621 tgtcggtcag cccataggta atagccagaa tgatgtgccg agagctggtg gaggcttgat
     1681 tgatacgcct cagcttctcg atggaagcac ccggaaaagc ggaaagccac gaaaatagac
     1741 tcgcttccac cccgaagcac tccggttgac tgcccccgta tcggaactta ttagtcatta
     1801 ttgtcatcga cctcggctct cagccggcaa ccctcttctt gccacacagc acacccttag
     1861 ccataaacac agcgaacacc agccttgcaa tcaaatactc ttattgcgat tcattctcat
     1921 cttttccaaa agactactat atatcaacca tagcgacaag ccgtccgaag acttaagcac
     1981 ggttgccgaa ccagctttcc cagaaccgag agcactaatc tctaattttc cagccctggc
     2041 aattgactga acgtccttaa caatatgcaa ctcattctca ttaaagtgaa ttccacgcct
     2101 cctcccggcc aagagaagcg ccccaccccc ggccaagggg ctat
//
LOCUS       DAOQNZ010000718         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33104_length_2144_cov_3.100048, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000718 DAOQNZ010000000
VERSION     DAOQNZ010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33104_length_2144_cov_3.100048"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2085)
                     /locus_tag="PKW73_12525"
     CDS             complement(<1..2085)
                     /locus_tag="PKW73_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HNA74161.1"
                     /translation="MHNYFFKHKGTLAVMRSLLLAGSALSLIAGPTLIPTVSARNLLA
                     ATGTGTVGTTGSRETAKSPFTTEGSGTLTAMTGPGQKLGACPLKHTAVSADISGYVAR
                     VTVKQTFSNPFTKKIEAVYTFPLAENGAVDEMVMKVGTRTIHGTIKKREEARQIYEDA
                     MAQGHVASLLEQERSNIFTQSVANIEPGKDIEVTISYIETLPYESGKYSFAFPTVVGP
                     RFIPGNAVSGGAQGGGFAPNTNQVPDASRITPTPVKEGERAGHDLSIDIHLNAGMPVS
                     AVSSALHEVSIKNNGNSSADISLVNKNTIPNKDFVLSWNVAEDTLKSGYLTYKNKEDK
                     DGYFTLMLMPPKRVTPATTAPKELIFVIDCSGSQSGAPLQKAKDAMLYMLDHMNSNDT
                     FQIITFNNNVETFSAKPELSSNEMKERARDFINKMQARGGTWMAPAVEKACSTEADSH
                     RLRIVTFMTDGYVGNDFEILGLVKKLRGNSRWFPFGTGNSVNRMLIDGMAKEGGGEAE
                     YVLLNSSAQEVGKKFYERIGSPVLTDVKLSFDGLEVKEVFPKEVSDVYAERPLYFKGR
                     YTAPRAGTITLTGFSAGKPYKQTMHVNFPEADKKNPGIASLWARAKVDRLMSEDWFGA
                     QQGQVNKEIKDEIIATALKHHIMTQYTSFVAVDDAVKTKGDKAEKVNVPIEMPDGVSR
                     EGVFGNAPGSAMP"
BASE COUNT          396 a          525 c          643 g          580 t
ORIGIN      
        1 gggcatggcg ctgccgggag cattgccgaa taccccttct cggcttacgc cgtcgggcat
       61 ttcaataggt acatttacct tttcggcctt gtcgcccttg gtcttcaccg cgtcgtccac
      121 ggctacgaag gaagtgtact gggtcatgat gtggtgcttg agagcggtgg cgatgatctc
      181 gtctttgatc tctttattga cctgcccctg ctgagcgccg aaccagtctt ccgacatgag
      241 ccggtctacc ttggctctgg cccagagaga ggcaatgccc gggtttttct tgtcggcttc
      301 cgggaaattg acgtgcatgg tttgtttgta cggtttgccg gcgctgaagc cggtcagggt
      361 gatggtgccg gctctggggg cggtatagcg ccctttgaag tagagcggtc tttccgcgta
      421 aacgtcggat acttctttgg gaaatacctc tttcacttcc agtccgtcga aggaaagttt
      481 gacatcggtg agcacggggc tgccgatgcg ctcatagaat ttcttgccta cttcctgggc
      541 ggaggaattg agcagaacgt attccgcttc gccgccgccc tctttagcca tgccgtcgat
      601 gagcatgcga ttgacactat tgccggtgcc gaaggggaac cagcgggaat tgcctctcag
      661 ctttttcacc agaccgagaa tttcaaaatc attgccgaca tagccgtcgg tcatgaatgt
      721 gacgattctc agtctgtggc tgtccgcttc cgtggaacaa gctttctcca ccgccggtgc
      781 catccaggtg ccgccccggg cctgcatttt gtttatgaaa tcgcgagccc gctctttcat
      841 ctcattgctc gatagttcgg gtttggctga gaaagtttca acattattat tgaaagtaat
      901 gatctggaag gtgtcgttgc tgttcatatg gtcgagcata taaagcatgg cgtctttggc
      961 cttttgcagc ggtgcgccgc tttgcgagcc ggaacagtcg atgacgaaga tcagctcttt
     1021 gggagccgtg gtggccgggg tgacgcgttt gggcggcatc agcatcaggg tgaagtagcc
     1081 gtccttgtct tctttgtttt tgtaggtcag atagccgctt ttgagtgtgt cttcggccac
     1141 attccagctt agaacgaagt ctttgttggg aattgtattt ttgttgacga ggcttatgtc
     1201 ggcactgctg ttgccgttat tcttgatgga gacttcgtgc agggccgagc ttacggcgct
     1261 gaccggcatg ccggcgttga gatggatgtc gatggaaaga tcgtgaccgg ccctctcgcc
     1321 ttctttgacc ggtgtgggtg tgatgcggga ggcatccggc acttgattgg tattgggagc
     1381 aaagccgccg ccctgggcgc cgcctgaaac tgcattacct ggaatgaagc gaggtccgac
     1441 cacggtgggg aaggcaaagc tgtatttgcc agactcgtaa ggcagggtct cgatataact
     1501 gatggtcact tcaatgtctt tgcccggttc gatattggcc accgattggg tgaaaatatt
     1561 gctgcgctct tgttctaaaa gtgaagcaac gtgcccctgt gccatggcgt cttcgtatat
     1621 ttgtctggct tcctcgcgtt ttttgatagt accgtgtatg gtgcgggtgc cgaccttcat
     1681 caccatctcg tccacggcgc cgttttcagc cagaggaaaa gtatagacgg cttctatttt
     1741 ctttgtgaaa gggttggaga aggtctgctt caccgttact cttgccacgt agcctgaaat
     1801 gtcggcactg acggcggtat gcttgagcgg acaggcaccg agtttttgac cgggaccggt
     1861 catggctgtg agggtgccgg aaccttcagt ggtgaagggg cttttggctg tttcccggct
     1921 gccggttgtg cctaccgtgc cggtgccagt tgcagccaat aggttgcggg cggagaccgt
     1981 gggaatgaga gtcggaccgg ctatcaggct gagagccgag cctgccagta gcaagcttct
     2041 catgacggcc agtgtgccct tgtgtttgaa aaaatagttg tgcataaaac tccctcttaa
     2101 ggctttcaag ccggagaaga atggtgagat gtagaaccct tggg
//
LOCUS       DAOQNZ010000719         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33177_length_2141_cov_4.548418, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000719 DAOQNZ010000000
VERSION     DAOQNZ010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33177_length_2141_cov_4.548418"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /gene="thiL"
                     /locus_tag="PKW73_12530"
     CDS             complement(<1..717)
                     /gene="thiL"
                     /locus_tag="PKW73_12530"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNA74162.1"
                     /translation="MPSFERELKLIAGIKEWTGSPFIGDDCAVLPGRGLVTTDTLVEG
                     THFRLEWTDLLALGWKACAVNLSDIAAMAGRPRFLTVALTMPGNFGTSELRSLYLGLC
                     DCAARYRARIVGGDLTSGEKLVINVTAMGDVHEAGIMRRSTASVGEIVVVTGSFGGSG
                     AALSLLNRLSQSETRPGPLGASLIPLALLRPGSHSQKLLQMHFRPEPRLSSAWHLVEL
                     TGGRGSLMDASDGLADGLVQI"
     gene            872..1126
                     /locus_tag="PKW73_12535"
     CDS             872..1126
                     /locus_tag="PKW73_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNA74163.1"
                     /translation="MNPIIQIIDGVISIFSMALILWCLLTWFPNINWYDQPWRTLDKI
                     VRPVLAPVKKVIPPIGNIDISAMVLAIALGFIRNILHALF"
     gene            1278..>2141
                     /locus_tag="PKW73_12540"
     CDS             1278..>2141
                     /locus_tag="PKW73_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74164.1"
                     /translation="MPTRRAFLFSSMSYLAGSLFLPSALEPAQARKAPRTQNPFAGGG
                     GPRTLFKEEWPPAILPAHVGVIDKGYLCFTDDLGRLAVVDMRKPAAGKPPFKVLASLS
                     GLGNKVIDFAVGPFAAYGLVYRENDNHEAVVTLVSVSLTPATAPAIINELPLTRLTDA
                     QAITTSGDLICLAGTASSGESLVSIYGAPNRRGESKEPTYIASLSVNQPIRALDLTEK
                     QLTILSSDTSGQRSQLDYVLVSNAGAPEVQSAVKMEGDYRVLSRFKDTVIVAGYEKGS
                     RPGKGRCFARTV"
BASE COUNT          485 a          654 c          564 g          438 t
ORIGIN      
        1 tatctgcacc agcccgtcgg ccagaccgtc ggaagcgtcc atgagcgaac cgcgcccgcc
       61 ggtcagttcc acaagatgcc aggcactact gagacgcggc tcgggccgaa aatgcatttg
      121 caagagcttc tgggaatgac tgccgggacg caagagcgcc aggggtataa gggaagcgcc
      181 cagcggtccg ggcctggtct cgctctggga aaggcgattc aagagactga gggcggcgcc
      241 gctgccgccg aaactgcctg tcaccaccac aatttcaccg acggaagcag tgctgcgccg
      301 catgataccg gcttcgtgca cgtcacccat ggccgtgaca ttgatcacga gcttttcacc
      361 ggaagtgaga tcgccgccca ctatgcgggc ccggtaccgg gcagcgcagt cacaaagacc
      421 caggtaaaga ctgcgtaatt cggaggtgcc gaaattaccg ggcatggtga gagccaccgt
      481 caggaaacgg ggacgaccgg ccatggccgc aatatcgctc aggttcacgg cacaggcttt
      541 ccagcctaga gccagaagat cggtccactc cagacggaag tgggtgccct ccaccagggt
      601 atcggtggtg acaagaccgc gccccggcaa aacagcacaa tcatcaccga tgaaaggact
      661 gccggtccat tctttaattc cggcgatcaa tttaagttct ctctcgaaag agggcataaa
      721 ttccgcctcg tacagctcgg gccgcacaca agtcaaatcg ccgttccagc atgacggccg
      781 gggcgaataa actcaatagg caagccccta atctcaacaa atgcttcgca aatgctacaa
      841 tcagccttgg aagtgcttaa gggccgctca catgaatcca atcattcaaa ttatcgatgg
      901 tgttatcagt attttcagca tggcgctgat tctctggtgc ctgctcacct ggtttcccaa
      961 tatcaattgg tacgatcagc cctggcgcac cctcgataaa attgtgcgac cggtgctggc
     1021 gcccgttaaa aaggtgattc cgcccatcgg caatatcgac atctcggcca tggttctggc
     1081 tatagctcta ggtttcatcc gaaacatact gcatgccttg ttctaagggc accggtgcaa
     1141 ttgttaaatt ccttgagtgt ttctctctca ttatggctct agaataatga ccgccggtat
     1201 ctgccgcggg ctatcctcgg tcttggctcg caagagaaac agctagcggc gcaaaagaaa
     1261 ctaccgctgg gaagacaatg cctacaagac gcgcctttct cttttcttca atgagctatc
     1321 tggctggaag cctgtttttg ccgtccgccc tggaaccggc tcaggcccgc aaggctccgc
     1381 gcacccaaaa tccctttgcc ggcggtggcg gtccccgtac ccttttcaaa gaagagtggc
     1441 cgccggcgat tctaccggcc catgtgggtg tcatcgataa aggttatctt tgtttcaccg
     1501 acgacctcgg tcgcctggcc gtagtagaca tgcgcaaacc ggcggcaggc aagccgccct
     1561 tcaaggtgct ggcttccctg agcgggcttg gaaacaaggt tatcgacttt gccgtcggac
     1621 cttttgccgc ttacggattg gtttatcgag aaaacgacaa ccatgaagcc gtggtcaccc
     1681 tggtttcagt ttctctcacc ccggccacgg cgccggccat catcaatgaa ctgcccttaa
     1741 ccagactgac cgacgcccag gccatcacca ccagcggcga cctgatttgc ctggccggca
     1801 ccgcttcctc cggcgaatcc cttgtctcca tatatggtgc acctaatcgc cgcggcgaaa
     1861 gcaaagagcc cacatatatt gccagcctca gcgtcaacca accgattcgg gccctcgacc
     1921 ttaccgaaaa acaactgacc attctctcct ccgacaccag tggccagcgc tctcaactgg
     1981 actacgtact ggtctccaac gccggagcac cggaagtgca gagcgcggta aagatggaag
     2041 gcgattaccg tgttctgtct cgcttcaaag acaccgtaat tgtggccggc tatgaaaagg
     2101 gcagccgccc cggcaaaggt cgctgctttg ctcgcacggt c
//
LOCUS       DAOQNZ010000720         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33197_length_2141_cov_2.611697, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000720 DAOQNZ010000000
VERSION     DAOQNZ010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33197_length_2141_cov_2.611697"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PKW73_12545"
     CDS             complement(<1..807)
                     /locus_tag="PKW73_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA74165.1"
                     /translation="MNSKSNCHWHALAACWLGCLFDGLDASIFTIVLYPALSELLGTS
                     SHAVVGYHGALIIAVFLVGWALGGVLFGRLCDQIGRVRTLIISILLYTLATGLCSLSH
                     NWQELAFYRFLVGLGIGGELSVGTVVLSEHWQGKPRLHAVSFMATSWGFGYFATALMT
                     AGLGGASWRYLFAAGVLPALITLYIRLKLRESKSFSNLKRQRRTKAQMNREPLREVLG
                     GEYLRSLLVVIAITSSGIVGYWTVLSWIPSWINQMVGTDAVYERSLAMMLM"
     gene            complement(990..1943)
                     /gene="cysK"
                     /locus_tag="PKW73_12550"
     CDS             complement(990..1943)
                     /gene="cysK"
                     /locus_tag="PKW73_12550"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNA74166.1"
                     /translation="MPLLTNISEAVGATPLISLSKIAAGLKARVMVKVESMNPLGSVK
                     DRIGISMVLDAESRGLLVPGKTTLVEPTSGNTGIALAFVAAARGYKLIITMPETMSSE
                     RRRMLTVLGAELVLTPGTEGMKGAVARAEQILKELPDSHMLQQFDNPANPMAHYRTTG
                     PEIWQDTDGKVDMFVAGVGTGGTITGTGRFLKEKNSQIKVVAVEPADSPVLTQKRAGQ
                     EVKPGPHKIQGIGAGFIPSVLDLSVIDDVFRVQNEEAFAMGRRLPREEGILAGISSGA
                     NVHAAIELAKLEENAGKTIVTVVCSTGERYLSTALFEPDLI"
     gene            complement(1944..>2141)
                     /locus_tag="PKW73_12555"
     CDS             complement(1944..>2141)
                     /locus_tag="PKW73_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74167.1"
                     /translation="YKFALRHAQALIARADKMIKDGESRKDDKMLKKGQILKGIGERT
                     LENLKASNPLPEAQNALKDKQ"
BASE COUNT          505 a          580 c          575 g          481 t
ORIGIN      
        1 cattaacatc atagccagac tgcgttcata gactgcatcg gttccgacca tttgatttat
       61 ccaggatggt atccaggaga gtacggtcca gtatccaacg atgccgctgc tggtaatggc
      121 aatgaccact agaagagacc ggagatactc accgccgagc acttcccgaa gtggctctct
      181 gttcatttgt gccttggttc ggcgctgtct cttgaggttg gagaatgact tagactctct
      241 tagtttcagc cgtatgtaga gagtgataag ggccggcagt acgccggcag caaacaaata
      301 gcgccagcta gctccgccta gaccagcggt catgagggct gtagcgaagt agccgaagcc
      361 ccaggatgtt gccatgaaag acactgcatg cagtcttggt ttcccctgcc agtgttcgga
      421 taacactact gttcccaccg aaagctcccc accgatgccc agaccaacca gaaagcggta
      481 aaaggcaagt tcctgccagt tgtgactgag agaacagaga ccggtagcta gagtgtagag
      541 aagaatgcta atgatcaggg ttctaactcg gcctatttga tcacagaggc gaccaaacag
      601 aacaccacca agagcccagc ccacaagaaa aacagcaatg atcaatgcac cgtggtagcc
      661 taccacggca tgagaactgg tgcccaagag ctcggagaga gcagggtata ggacaatggt
      721 aaaaatcgag gcatcgagac catcaaagag acatcccagc caacatgccg ctaaggcatg
      781 ccagtgacag ttcgatttcg aattcatcgg tgtacctctc tggaatcttg cctgcagttt
      841 gcgtcagcac cggcgtgctg tcaagagatg tgggtcaagc aaaaactcag aaggagagca
      901 actttcggca cagtttgctg ccgctagaga taccctccgg catcaccatg cgacaattcg
      961 gcaaagtctg ccaagataaa atctaagggc taaatcagat ccggttcaaa caaagccgtt
     1021 gatagataac gctcaccggt actgcacacc actgtgacga ttgttttgcc cgcgttttct
     1081 tccaatttgg ctagttcgat ggcggcatgt acattggcac cactggagat accagcgagg
     1141 atgccctctt cccgcggtag cctgcggccc atagcaaagg cttcctcgtt ctgcacccgg
     1201 aagacatcat ctattaccga taaatctagc acagagggaa tgaaaccggc gccgatgcct
     1261 tgaattttat ggggaccggg cttcacttcc tgtccggccc gcttttgggt gagcaccgga
     1321 ctatcagccg gttccaccgc caccacttta atttgcgaat tcttttcctt gaggaaacga
     1381 ccggtaccgg tgatggtgcc accggtgccc acgccggcta caaacatgtc taccttgccg
     1441 tctgtgtcct gccaaatttc cggtccggtg gtccggtagt gagccatggg attggcggga
     1501 ttatcgaact gctgcaacat atgactatcg ggcagttcct tgagaatttg ctcggctctg
     1561 gctacagcac ccttcatgcc ttcggtaccg ggggtgagca ccagctcagc gcccagcacg
     1621 gtcagcatac gccgtctttc ggaggacatg gtctcgggca tggtaattat cagtttatag
     1681 ccccgggcgg cagccacaaa agcaagggcg atgcccgtgt tgccggaggt gggctccacc
     1741 agggtggtct taccaggcac aagcagacca cggctttcgg cgtccaggac catggaaata
     1801 ccgatgcggt ccttgaccga gcccagcgga ttcatggact ccaccttgac catcacacgg
     1861 gctttcagcc cggcagcaat cttggacagg ctgataaggg gtgtggcccc gacggcctca
     1921 gatatattgg ttaagagagg catttattgc ttgtctttca gagcgttttg agcttccggc
     1981 aggggattgg aagctttcag gttttccagg gtgcgctcgc cgatgccctt caagatctgc
     2041 cccttcttga gcattttatc gtctttgcgg ctttccccgt ctttgatcat tttgtcggcg
     2101 cgggcaatga gggcctgggc gtgcctgagg gcaaacttgt a
//
LOCUS       DAOQNZ010000721         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33208_length_2140_cov_3.831175, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000721 DAOQNZ010000000
VERSION     DAOQNZ010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33208_length_2140_cov_3.831175"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKW73_12560"
     CDS             complement(<1..494)
                     /locus_tag="PKW73_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNA74168.1"
                     /translation="MADRTKGSPEHSNNSAISSSSMDAIAEIIRSIPRGDHLTAPEVY
                     ELAKQRGLKLSLSTVYRTLNKLKVVGDVITVSGDRGLRYESVEDQAHDHLICLGCGLT
                     IEFFDDLIRGFGHSVALRKGFEHTTSRFDILGYCEKCKGNDEGHKQEQMLDNLLGAIE
                     KTEAA"
     gene            881..1804
                     /locus_tag="PKW73_12565"
     CDS             881..1804
                     /locus_tag="PKW73_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNA74169.1"
                     /translation="MKITINKESAVPIRDQLVEQLGLSIASGSLRPHEKLPSIRALAD
                     RLGVHYSTITAAYNHLAEVGLLEVRQGSGVRVAASGRRVENESTALSLEEMFFDFLAR
                     ASENGYSSSEVSKCMDKLRHRPAVKRILVVDGNQDFHPLIKGELSPHFQLPVECLLPQ
                     QLLSDLKQAEEALVVSSLYHLFSFQKHIKDLTRLVACNIEPARNELEAVYRLPKASLL
                     ALVSVSPHLMQMAGKLVAAMRGEEIAVRAVVRDDETEIKYLVKHADLFLCDSISREVV
                     SRYAPPAKMLVFNLYSSTTIDLIKERLAKWG"
BASE COUNT          467 a          520 c          548 g          605 t
ORIGIN      
        1 gccgcttccg ttttttcgat agccccgagc aggttgtcca gcatctgctc ttgtttgtgt
       61 ccttcatcgt tgcctttgca cttttcgcaa tagcccaata tgtcgaagcg gctggtggtg
      121 tgctcgaaac ctttacgaag cgctacggaa tgaccgaagc cgcgaatcaa atcatcaaaa
      181 aactcaatgg tcaatccgca acccaggcag atcaaatgat catgggcctg atcttctact
      241 gattcgtagc gcagaccccg gtcgcccgat actgtgatta catctcccac tacttttagt
      301 ttattgaggg tgcggtagac cgtagagaga ctcagcttta agccccgctg tttggccagt
      361 tcgtaaacct cgggcgctgt caagtgatcc ccccggggaa tggagcggat tatttcggca
      421 atggcatcca tcgacgaact acttatagcg gaattgttgg agtgctcggg agagcctttt
      481 gttctgtcag ccatgccggt tgtttccaaa actcattatt gtttgatact tattgcgaat
      541 gattgcataa tttcatccgg gggacaactg aagaaagggc acaagtaggt ataacttgat
      601 acttttgtct cctggcaagt cttgacttgg ctgttccctg atgctaatct ggatttgttg
      661 ctaatgagaa tacttcgcat ccatcagcaa agttcatgcg aggaattcta cagatgcaca
      721 ggagccccac ggtccactga ggcttgtcag tagaaatagg gacagatttc tatctggcgc
      781 aaactataaa taagagagcg tcctttctaa gcagagaagg gcgctctctt ctattttgat
      841 ggcatgccat ataattggct cttattgcaa ggatcaggct ttgaaaatta ctatcaacaa
      901 agaaagtgcg gttccgattc gagaccagtt ggtcgagcaa ctgggtctgt ccatcgcttc
      961 cggttccttg cgcccccacg agaaattgcc aagcataaga gccttagccg accgtctcgg
     1021 cgttcactac agcaccatta cggctgctta caatcacctg gccgaagtcg gactgctgga
     1081 ggtgcggcag ggcagcggcg tgcgggtagc ggccagtggc aggcgggtag aaaacgagag
     1141 cacggcgctt tctctggagg aaatgttttt tgattttctc gcccgggcct ctgaaaatgg
     1201 ctattcgtct tcagaagtaa gcaagtgtat ggataagttg cggcatcgcc cggccgtcaa
     1261 acgtattctt gtggtggacg gcaatcagga cttccatcct ctcatcaaag gcgaactttc
     1321 gccccacttc cagttaccgg tggaatgcct tttgcctcaa caattgctct cggacttgaa
     1381 gcaggcggaa gaggctctgg tggtttccag tctctatcat ctgttctctt tccagaagca
     1441 cattaaggat ctgactcgcc tggtcgcctg caatatcgag ccggccagaa acgaattgga
     1501 agctgtatat agattgccga aagcctcact tctggccctg gtatcggtca gtcctcacct
     1561 catgcagatg gccggaaagt tggtagcggc catgcgcggc gaagagattg ccgtgcgggc
     1621 cgttgtgcgc gacgacgaga cggaaatcaa atatcttgtc aagcatgccg atttgttttt
     1681 gtgcgacagc atcagccggg aagtggtgag ccgctatgct ccgcctgcga agatgcttgt
     1741 tttcaatctc tattcgtcca caaccataga tttgatcaaa gagcgcctgg ccaagtgggg
     1801 ctgatagtcc cggtttgcag gaatttctag tttctggcgg agcttgtctt caggggttaa
     1861 cctggaagca gatggtttta ctgatttttt tcaggtggat ccggtggatt tagaataatg
     1921 tctagatggt tctttgcctt tgcctgtctg tttcttctct tctgtcaggg tttgtgctta
     1981 agcctctgta ccttctctta tctagggcgg catggacagt tttttgagtt gactactcac
     2041 tttcagccat tttactttgt ctttgctttg ctcttacttg gcctgtcgct ttgcctttgt
     2101 ttgttgctca agccggtgca aaagcgcatt tccttcgccg
//
LOCUS       DAOQNZ010000722         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33286_length_2137_cov_3.696926, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000722 DAOQNZ010000000
VERSION     DAOQNZ010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33286_length_2137_cov_3.696926"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKW73_12570"
     CDS             complement(<1..404)
                     /locus_tag="PKW73_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74170.1"
                     /translation="MSACPRKLGSYFQENELKSELCQGGGNVWSDELSMAWNKGKANV
                     VAVSAQMDAGGGLVLNKSMLPNLAIEENETLVLTSAHIVDSKTSILVGTYNGAGEMGS
                     QGMDEAQLVALDREKDLALLKVSLSESAGGPLA"
     gene            597..1481
                     /locus_tag="PKW73_12575"
     CDS             597..1481
                     /locus_tag="PKW73_12575"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNA74171.1"
                     /translation="MQNDFLSAKKQLPSLGVGLGLRTELAESIFAETTTIDFLEIIPE
                     QYMGKGGESRERLEKACSLYQAVSHGVNLSLGSTDELDWSYLRTLKQFVNDIRAPWFS
                     DHVCFASLGQVYLHELLPVPFNRTTIEHMTRRIKQVKAYMELPFIIENTTYYMNVPGS
                     AMSEAQFLSELLEQADCGLLLDINNVYVNCINHGLDPYGFLDQLPLERTVQIHMAGHK
                     QSGDIVLDTHGSSIADPVYDLLKYALGRPGVKVHAISIERDQNQPAFTELKEELAAIK
                     SIYKNSTSAALSVKKAGA"
     gene            1482..>2137
                     /locus_tag="PKW73_12580"
     CDS             1482..>2137
                     /locus_tag="PKW73_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74172.1"
                     /translation="MDHSNLKETEEILLRLFTDREYLYQVSSEGRLPNYPNYSFDRRG
                     ASIYAAVLQTNRLETMKAIYPRTARVLADQFERIIKDYGLALNKLVKSETAGTKNFLL
                     NKLGIYFPKYFQLAEQQLSSKYPYLQNLIQFEAATAELADMEEKSKAAQTDKIVSQTA
                     RQEWFRVNPCLMIFPFQYDIAELIRAIDESENPGDLQFPAQEMVMLMLRNPSTYKIRL
                     L"
BASE COUNT          593 a          584 c          468 g          492 t
ORIGIN      
        1 gccaggggcc ctccggcgct ctcggaaagc gagaccttca atagagccag atccttttct
       61 cgatcaagag caaccaattg ggcctcgtcc atgccctggc tgcccatttc tccggcaccg
      121 ttataagtac cgaccagaat ggaagtcttg ctatccacaa tgtgagcaga cgtgagaacc
      181 agagtttcat tttcctcgat agccagattc ggcagcatcg acttattcag caccaggccg
      241 ccgccggcgt ccatctgagc actaaccgcc actacattag ctttgccctt attccaggcc
      301 atggaaagtt catcactcca gacattgccg ccgccctgac agagttcaga cttcagctca
      361 ttttcctgga aatagctacc cagtttgcga ggacatgcag acatttgcca ccaaccatga
      421 cggtgccacc accggcacta ttaccccaaa cggcaggata agatatcctg tctttaaacc
      481 atactgattt tgcgtgacca aaaacggact tttccgctca aattaaacat gcgcaaagca
      541 agtgaataag cgccttcagg tcttgccaac cagagctgcg cgattatact tgttgtatgc
      601 aaaacgactt tcttagcgca aaaaagcaat taccttctct gggtgttggt ctcggtctga
      661 gaaccgagct ggctgagagc atctttgcag aaacaacaac aatagacttc ctcgaaatca
      721 ttccggaaca atatatgggt aaaggcggcg aaagcaggga acgtctggaa aaagcctgct
      781 cgctctacca agccgtcagt cacggcgtca atctctcttt agggagcacc gatgaactcg
      841 actggtctta cttgcgaaca ctcaaacaat ttgtcaatga cattcgcgcg ccctggttca
      901 gcgaccatgt atgttttgcc agtcttggtc aagtctacct gcacgaattg ctgccggttc
      961 ctttcaaccg caccacaata gagcatatga ccaggcgcat caaacaggtg aaagcctata
     1021 tggaactacc gtttatcatt gaaaatacta cctattacat gaatgtgccg ggcagcgcca
     1081 tgagcgaggc acagtttcta agtgaattac ttgagcaagc cgactgtggt cttctgctcg
     1141 acatcaacaa tgtctatgtc aattgcatca atcatggctt agatccctac ggttttctcg
     1201 accagctacc gctggagcgt accgtacaga ttcatatggc cgggcacaag cagagcggcg
     1261 atattgttct cgatacacac ggcagttcca tagcagatcc ggtttacgac ttgctcaaat
     1321 acgccctcgg cagacccggc gtaaaagtgc acgctatctc catagaacga gaccagaacc
     1381 aaccggcctt caccgaacta aaggaggagc tggccgccat caaatccatc tacaaaaact
     1441 caacttcagc cgctctgtcg gtcaaaaagg caggtgcttg aatggaccac tcaaatctga
     1501 aagaaactga agagattcta cttcgcctct tcactgatag agagtacctt taccaggtaa
     1561 gttccgaagg ccgcctgccc aattatccaa actacagctt cgaccggcgc ggtgcctcaa
     1621 tttatgccgc tgttttgcag acaaaccggc tggaaaccat gaaagctatt tatccacgca
     1681 cagccagagt tctggccgac caattcgaaa gaatcatcaa agactacggt ctggccctga
     1741 acaagctcgt aaaatcagag acagccggca ccaagaactt tctcctgaac aaattgggca
     1801 tttacttccc caagtacttt cagttagcgg agcaacagct ctcaagcaag tatccctacc
     1861 ttcaaaatct cattcagttt gaagcagcca cggccgagct ggctgacatg gaggaaaagt
     1921 ctaaggccgc tcagacggat aaaatagttt cccagacagc aaggcaagaa tggttcagag
     1981 ttaacccctg tctcatgata tttccctttc aatacgatat cgcggagctc attagagcta
     2041 tagacgaaag tgaaaatcca ggcgatttgc agtttccggc gcaagagatg gtaatgttga
     2101 tgcttcgcaa tcccagtacc tataagatca ggctgct
//
LOCUS       DAOQNZ010000723         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33319_length_2136_cov_3.524267, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000723 DAOQNZ010000000
VERSION     DAOQNZ010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33319_length_2136_cov_3.524267"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PKW73_12585"
     CDS             <1..1033
                     /locus_tag="PKW73_12585"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74173.1"
                     /translation="PMGGLLEQADGTAWMSFYCTSMLGIALELAGDRPAYEDVASKFF
                     EHFVQIADAVHGACGEGLWCEDDGFYYDQLVIEDQAIPLRIRSIVGLIPLLAVEVIKE
                     ERIKSLPGFYKRMQWFIKHRQYLEEDLAKTRDSDIGPLRLLALVSKDKLIRILKYMLD
                     ENEFLSPYGIRSLSRIHEEHPYSFCAEGHTYTVSYVPAESETGAFGGNSNWRGPIWLP
                     LNFLLIEALERYHKFYGDEFTVECPVGSGQFMNLQQVALEIGHRIASLFLKTKTGFAP
                     WQEGQEALFASEHCRDLHLFYEHFHADSGRGLGASHQTGWTALITRLFDDFVRRENYS
                     QSSTGGKGK"
     gene            1030..1935
                     /locus_tag="PKW73_12590"
     CDS             1030..1935
                     /locus_tag="PKW73_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74174.1"
                     /translation="MSAAALYRYLPALAILLLVAGNGSSAQAAPDNAHTVIHSNNTAK
                     QPQKTNNKPLRILFLGNSLTAMSFMPWMVHEFVKTSGRPCDIEMLAFPGETLEGHYHT
                     VKTKMAVTSGKWDCIVLQEASEIRLEPKRAAQYAHLLTEAIKGKDLSRKAETPTKILF
                     FEAFADKGQTKHQEEIRLTTEAICRQTGATLVPVGELFAANLKEKPALNPYMSDNHHP
                     SASGSYLAACAIYRAIMGAKYKALKSSLPSKIAYVCQANDPYIPATESGQAPPPGTSF
                     TLVELKKADAAYLQNLAETQPKSQF"
BASE COUNT          564 a          633 c          514 g          425 t
ORIGIN      
        1 ccccatgggc ggtcttctcg aacaagcgga cggcaccgcc tggatgagtt tctactgtac
       61 ctctatgctg ggcatcgccc tggaactagc cggcgacaga ccggcttacg aggacgtcgc
      121 ctcaaagttt ttcgaacact tcgtgcaaat tgccgatgcc gtgcacggtg cctgtggcga
      181 aggtctatgg tgcgaagacg acggcttcta ttacgatcag ctagtaattg aagaccaggc
      241 tattccgctg cgcattcgct ccatcgtcgg tctgattccg cttctggcgg tggaagtaat
      301 caaagaagag cgcatcaaaa gcctgcccgg cttttataaa cgcatgcagt ggttcatcaa
      361 gcatcgccaa tatctggaag aagatttagc caaaacaaga gactcagaca ttggccccct
      421 gcgtctttta gctctggttt caaaagacaa actgattcgc atcctcaaat acatgctgga
      481 tgaaaatgag ttcctctccc cctacggcat acgctcactc tccagaattc atgaggagca
      541 tccctatagt ttctgcgcgg aagggcacac ttacacagtc tcttatgtac cggcggaaag
      601 cgaaaccggc gccttcggcg gcaactccaa ctggcgaggt cccatctggc tccccctcaa
      661 ctttctattg attgaagcgc tggagcgtta ccataaattt tacggcgacg aatttaccgt
      721 agaatgcccg gtgggctcgg gacaattcat gaacctgcag caagtggcgc ttgaaattgg
      781 gcaccggatt gcatcccttt tcctcaagac gaaaaccggc tttgccccct ggcaggaagg
      841 tcaggaagcc cttttcgctt ccgaacattg ccgggacctg catcttttct atgagcactt
      901 ccatgccgac agcggacgcg gcctcggcgc cagccaccag acaggctgga cggcactgat
      961 aaccagactc ttcgacgatt tcgtgcgccg ggaaaactac tcgcaaagta gcacgggagg
     1021 aaaaggaaag tgagcgccgc cgcactctac cgttacctgc cggcactcgc catattgctt
     1081 cttgtcgccg gcaacggcag ttcagcgcag gcggcgcctg ataacgccca cacggtcatc
     1141 cacagcaaca acaccgccaa acagccgcaa aaaaccaaca acaagccgct gcgcattctc
     1201 tttctgggaa atagcctcac ggccatgagc ttcatgccct ggatggtaca cgaatttgta
     1261 aaaacttcag gccgcccctg cgatatcgaa atgttggcct ttcccggtga aaccctggaa
     1321 ggtcactatc atacggtcaa aaccaaaatg gcagtcacaa gcggcaaatg ggactgcata
     1381 gtcttgcagg aagcctcgga gattcgactg gagcctaaac gagccgccca gtatgcccac
     1441 cttctcactg aagccatcaa aggcaaagac ctgagccgga aggcagaaac accaacaaag
     1501 attctcttct tcgaagcttt cgccgacaaa gggcaaacca aacaccagga agaaatccgc
     1561 ctcaccacag aagccatctg ccgccagacc ggcgctaccc tggtaccggt gggcgaactt
     1621 ttcgccgcca acctgaagga aaaaccagcc ctcaaccctt acatgagcga caaccatcac
     1681 ccttccgctt ccggcagcta cctggctgcc tgcgccatct atagagcaat catgggagct
     1741 aagtacaaag cgctcaaatc ctcgctacca agcaaaatag cctacgtttg ccaagccaac
     1801 gacccctaca ttccggccac agaaagcggc caggcgccgc cccctggcac aagcttcacc
     1861 ctcgtagaac tcaagaaagc cgacgccgcc tatctacaga acctggcgga gacccagcct
     1921 aagagccaat tttaagtttt tgaaattaag ttctggaaac aaaatactgt agttcgtacg
     1981 gtattcaaga agaccactgt tatactgctg tagctaccaa acagctaatg aggattgcca
     2041 tgaacgagac agcgcaagtg aaaatcaacg ggacagttaa agaactctcc ggcgctaagc
     2101 tcaaggtcgg catagtggga gccaccggca tggtgg
//
LOCUS       DAOQNZ010000724         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33350_length_2135_cov_3.540385, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000724 DAOQNZ010000000
VERSION     DAOQNZ010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33350_length_2135_cov_3.540385"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKW73_12595"
     CDS             complement(<1..187)
                     /locus_tag="PKW73_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74175.1"
                     /translation="MTISLTGLTLNGRYHVLGEVGMGKDAIVYKAHDQKLDIPVAIKA
                     LKDELPSEHHLQRFHREA"
     gene            1112..>2135
                     /locus_tag="PKW73_12600"
     CDS             1112..>2135
                     /locus_tag="PKW73_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74176.1"
                     /translation="MKALYSIIAASFLLMASSLAVFADTKDNKIAKKAEPEFYPWPNG
                     QPGMMDKKTKEFLESRADNPSQQKLDAMLQQVDRITVDGGPKERFSTKDAEKIKTFKE
                     SLKIVEDPKTFGHCMCWGDPQIFLYSGKKQIAHLSCQHGSAIRWNDGWKWDATLKDGS
                     RLTEWLAANGAPRLKRYVEDDRKRAEESERALKKWLAAMPPSLTPYTKEVLGEEAGWN
                     VFMPAGGKAGKPATQEPSSKYSKYWKALNAAYKDKDSQILALLTWFGSGAGPWSGFPS
                     YEELPERMLVEIDTKDILHAVQGKSLSKEQLDGTARYFAGWGFRQSKNELSLVPRTLQ
                     KALLAHV"
BASE COUNT          626 a          519 c          537 g          453 t
ORIGIN      
        1 cggcttcacg gtgaaagcgc tgcaaatgat gctcggaagg caattcatct ttgagagcct
       61 taatcgccac cggaatgtca agcttttgat catgggcttt gtagacgata gcgtccttgc
      121 ccatgcccac ttcaccaagc acatggtacc tgccgttgag cgtcagcccg gtaagagata
      181 tggtcataaa cttgtcctcg atgacagatt cgtgccctga attaaggacc aaattttagc
      241 gcaagcggcc cctcagagta tgaattgggg gataagtagc gcggacgacc ggcggcgctc
      301 cggaaagaca aatctaacgc caaagttata caatgggaat gcggaattcg aactattttc
      361 ctgagtggcg tggtcgaaag agaattacgg accgagtctg ccgaaagggc actcgccacc
      421 agccggttcc tagcattaag gccgctaaca aacaagatat cacggcgata aagctatcag
      481 cgccgaactc gcagccataa atcagcacac cacagttcca attgctgcca cctgaatgat
      541 caacctgcat catgaagagg caaaagagaa caagacacaa cttaaaaact gcccatgcca
      601 ttagcggcag ggcaagccag gcaaagattg atccgaatag tgtaagcagt gcctttctca
      661 aaacgctaca caccctcccg gccccgccca agcgctagca tataagataa gtttatagga
      721 actaaccctt tctggccatc aatactcatg ctccaagcca atgacctgat tcgattgatt
      781 gcagaaggga taattcgcca agttgatcta gagacaattg ccacgtcgag tcgagcagac
      841 tgcgtaaatg cgacatacag gtatctcacg gcttcctttg acactgctct atcctccgaa
      901 gtttttgctg ggtaaaggaa atgagctgaa tcggcagcca cctttaaagt gccccttcaa
      961 actcatatag cggaacggag gtaatggtgg cggtggaatc cgagccagtg ctactatgag
     1021 aaaaccatac ttcacgaacg acgaaaaatg aagcgaagag ttgcatgctg cggcttgcac
     1081 cccttcatgg tagagagtct tggagaatcg aatgaaagct ttatactcca ttatcgccgc
     1141 atccttcttg ctgatggcta gctcattggc ggtattcgct gatacgaaag acaataagat
     1201 agccaagaaa gccgaaccgg agttctatcc gtggccgaac ggtcaacccg gcatgatgga
     1261 caagaaaacg aaagagtttc tcgaatccag agctgataat cccagtcagc aaaaattaga
     1321 tgccatgctc cagcaagtcg acagaatcac tgtagatggt gggccgaagg agagattctc
     1381 cacaaaagac gccgagaaaa tcaaaacgtt taaggaatcg cttaagatag tcgaagatcc
     1441 aaaaacattc ggacactgca tgtgctgggg agatccacag attttcctat attcaggcaa
     1501 aaagcaaatt gcgcatcttt cttgccaaca tggttctgca attcgctgga acgacggctg
     1561 gaaatgggat gcaacactga aggacggttc gcgcctgact gaatggctgg ccgcaaatgg
     1621 agcgcctcga ttaaagaggt atgtagagga cgaccgaaag cgtgccgaag aatctgaacg
     1681 tgccttgaag aaatggctgg cagcaatgcc tcctagtctg acgccataca ctaaggaagt
     1741 gctgggcgag gaagccggct ggaatgtctt catgcccgcc ggaggaaaag cagggaaacc
     1801 ggcaactcaa gagccgtctt cgaaatattc gaagtattgg aaggctttga atgcagcgta
     1861 taaagacaag gactcacaga ttctggcctt gttgacctgg ttcggctcag gagccggacc
     1921 ctggagcgga tttccttcat acgaagagct gcccgaaaga atgctcgtcg aaattgatac
     1981 caaagacata ctgcacgccg ttcaaggcaa gagcctctcc aaagagcaat tagatggcac
     2041 cgctcgttac tttgccggct ggggttttcg tcagagcaaa aatgaattga gcctggttcc
     2101 acgtactctt cagaaagcgt tattggcaca tgtag
//
LOCUS       DAOQNZ010000725         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33395_length_2134_cov_2.433381, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000725 DAOQNZ010000000
VERSION     DAOQNZ010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33395_length_2134_cov_2.433381"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /gene="gcvPA"
                     /locus_tag="PKW73_12605"
     CDS             <1..1319
                     /gene="gcvPA"
                     /locus_tag="PKW73_12605"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNA74177.1"
                     /translation="SHIPQSVKAGPLSMADGLSEMELTMQLKELAGKNKPVSRQISFL
                     GGGSYNRYVPAAVPSIISRSEFATAYTPYQPEVSQGSLQAIYEFQTAICLLTGMDVAN
                     ASMYDGPTALAEAAYMAVRLSNKKGIVVSGAVNPEYKMVVETYARSCDLPLTELKDCL
                     QRGSTCPDAVKALKGQDIACLIVQYTNYFGIIEDLEALAGAAHEIGALLIVSTDPISL
                     GSLKAPGDMGADLVVGDAQSCGNFLSFGGPSAGYMACRQDYVRQLPGRLAGMTTDNRG
                     QTAFTLTLQTREQHIRRAKATSNICTNQALNALAMLVYLTCMGPQGLAELGQVSALRA
                     HYLKDKLTALPGVKLTFAEDKPFFNEFAITVKGAESVLERLKARDILGGILVEKYLPE
                     LKDTILIAVTEMNPLSQLDTFATAMQEVLAGADKNQSGAKEKVLTV"
     gene            1356..2099
                     /gene="lepB"
                     /locus_tag="PKW73_12610"
     CDS             1356..2099
                     /gene="lepB"
                     /locus_tag="PKW73_12610"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNA74178.1"
                     /translation="MTATQAPPQTEKPTPGGFLTGFLKEAIELIVVTLALLIAIRWGI
                     AEARYIPSSSMEPTLQINDRLIVEKISGHLGKKIERGDILVFYPPKEEMGGQDLSNNP
                     LHIMGRLTGLPCFPNDPAFIKRCIGIPGDQIRVQANQGVYINQQLLDESAYVKEPPAY
                     NLNVMGDIKGRNALGMFIQAERDPKKQMDPIIVPAGHLFMMGDNRNNSEDSHVWGFLD
                     QRRVIGRAYLLIWRRLEAPKYPISLQSGE"
BASE COUNT          516 a          637 c          540 g          441 t
ORIGIN      
        1 tgagccacat tccccagtcc gtcaaagccg gtcccctctc catggccgac ggtctttccg
       61 aaatggaact gaccatgcag ttgaaagaac tggccggcaa aaacaaaccg gtctccagac
      121 aaatatcctt cctgggcggc ggcagctata accgctatgt gcctgcggct gttccttcta
      181 taatttcccg ctcggaattt gccaccgctt acacccccta ccagcctgaa gtctcccagg
      241 gcagcttgca ggccatttac gagtttcaga cggcaatctg cctgctcacc ggcatggacg
      301 tggccaacgc ttccatgtac gacggtccta ccgccctggc cgaggctgcc tacatggcag
      361 tcaggctgag caataaaaaa ggaatcgtag tctcaggcgc cgtcaacccc gaatacaaga
      421 tggtggttga gacttacgcc cgctcctgcg atctgcccct gaccgaattg aaagattgct
      481 tgcagcgcgg cagtacctgc cccgatgccg tcaaagcctt gaaaggtcaa gatattgcct
      541 gccttatcgt gcaatacacc aattacttcg gcatcatcga agacctggaa gcactggccg
      601 gcgccgccca tgaaatcggt gccctgctca ttgtttccac agatccgatc agcctgggca
      661 gcctcaaggc accgggcgac atgggcgccg acctggtggt gggcgacgct cagagctgcg
      721 gcaacttcct cagcttcggc ggaccgtcag ccggctacat ggcttgcaga caggactacg
      781 tcagacaact gccgggcaga ctggccggta tgacaacgga taaccgcggg cagacggctt
      841 ttaccctcac tctgcaaact agagagcagc atattcgccg ggctaaagcc acttccaata
      901 tctgcaccaa ccaggcgctc aacgccctgg ccatgctggt ttacctgacc tgcatgggtc
      961 cccagggtct tgccgaactg ggacaggtaa gtgccttacg cgctcattac ctgaaagaca
     1021 aactgaccgc cctgcccgga gtgaagttga ctttcgccga agacaaacct ttcttcaatg
     1081 aattcgccat caccgtgaaa ggcgccgaga gcgtgcttga aagattgaaa gcgcgcgaca
     1141 ttctgggcgg cattctggtt gagaaatatc taccggaatt gaaggacacc attttgattg
     1201 ccgttactga aatgaacccc ttgagccagc tcgatacctt cgccacagct atgcaggaag
     1261 tgctggccgg agcggacaaa aatcaaagcg gcgcgaagga aaaggttttg actgtataat
     1321 caaaatttcc atggttttta ataaggacct ttccattgac cgcgacacaa gcgccgcccc
     1381 aaactgaaaa gcctacacca ggcggatttc tcaccggttt cctcaaggaa gccatagagc
     1441 ttatcgtcgt caccctggct ctgctcatag caatacgctg gggcatcgcc gaggcccgct
     1501 acatccccag cagttccatg gagccgactc tgcagatcaa cgaccggttg attgtggaga
     1561 aaatctccgg tcacctgggc aagaagatcg agcgcggcga catcctcgtt ttctaccccc
     1621 ctaaagaaga gatgggcggt caggacctga gcaacaaccc gctccatatc atgggcagac
     1681 tgaccggttt gccttgcttc cccaatgatc cggctttcat caaacgctgc atcggcatac
     1741 caggcgacca gatccgggtg caagccaatc agggcgtcta tatcaatcag caactgctcg
     1801 acgaatccgc ctacgtgaaa gagccccctg cttacaacct gaatgtcatg ggcgatatca
     1861 agggacgcaa tgctcttggt atgttcatac aggccgagcg tgaccccaag aagcaaatgg
     1921 accccataat tgtgccggcc ggtcacctct tcatgatggg cgataaccgc aacaactctg
     1981 aagacagcca tgtctggggt ttcttagacc agagaagggt aatcggtcgg gcttatctgc
     2041 tcatctggcg ccgactggaa gctcccaaat acccaatttc tctgcagtcc ggtgaataac
     2101 gcttactgct aagccgcaat taggttagct ggac
//
LOCUS       DAOQNZ010000726         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33417_length_2133_cov_2.966795, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000726 DAOQNZ010000000
VERSION     DAOQNZ010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33417_length_2133_cov_2.966795"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..302)
                     /locus_tag="PKW73_12615"
     CDS             complement(78..302)
                     /locus_tag="PKW73_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74179.1"
                     /translation="MSEFLTYANGKGAVSEEKYSAPRLKQPGLEADRKERAKPEEEQL
                     MLKRLEERHRELLLWLMNLELRLPPYREPA"
     gene            complement(502..1107)
                     /gene="rdgB"
                     /locus_tag="PKW73_12620"
     CDS             complement(502..1107)
                     /gene="rdgB"
                     /locus_tag="PKW73_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNA74180.1"
                     /translation="MTVTEKEIELTLGTTNAGKLKEFLAMAREFHLKLNCHLAPPGFD
                     PEETGSTFEENALIKAKAAASLTGQLSLADDSGLTVEALNGRPGIYSARYAPGTDADR
                     RTKLLKELADVPAGKRQAAFVCALALVSADGQVLFSKQVEWPGEIAFQEKGEHGFGFD
                     PIFVPEGHTLTAAQLSPAVKNQISHRGVAFQQLLAYLAGQS"
     gene            complement(1115..>2133)
                     /locus_tag="PKW73_12625"
     CDS             complement(1115..>2133)
                     /locus_tag="PKW73_12625"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HNA74181.1"
                     /translation="VGHRCIGARINDRIVPLNSVLKNGDIVEIITSKNAQPKMDWLNF
                     ANTHGAKNRIRQWFKKHHREEHIQQGRQMLEAELGRGTLEEFLKSDKLKEVGKRLNIA
                     DANDILAAVGYGDLSINQVVNRIREVDQAEKIQKKGYAVPTPQNEKPSNISSLGGLLH
                     HLAKCCQPVPGEPIVGVVTRGSGIAVHRIDCNNLLKIEENRRMAVDWSKERNTNYPAG
                     LQVECLDRVGIAGDILKKVSDHKVNLRDLRVETHRDKKTATIFLLLDVNDIEQLARVS
                     QAISQISDVIRVYRKDHRKRSIAGPSADSKAKSGGKGQVTPRPAPRQGEEQKARRNGK
                     KKEE"
BASE COUNT          381 a          563 c          596 g          593 t
ORIGIN      
        1 gagcataggc atgcttgagg gcttcgcttg cggccccgac catggcatcg atggtcttct
       61 ggtccagctt catcaagtta agccggttcc ctgtaaggag gtaatcgcag ttccaggttc
      121 atgagccaga gcaggagttc tctatgcctt tcttccaggc gcttgagcat gagctgctct
      181 tcttccggct tggcgcgttc cttccggtca gcttctagac cgggctgctt caggcgcggg
      241 gcggagtact tctcttccga gacagcgcct ttgccgtttg cgtaagtaag gaattcggac
      301 atggttctcc tcggagcgcc gacatttctg tagcctggac tacattgaca gcttatctct
      361 tgtgaaccgt tattacctgt agccttttta atggctgcat gaaagtattc taaatcttcc
      421 ctgtatgccg cctatagcca gcgggcacgc gggctttcgt aacgaattgt cggctctatg
      481 ggaaggcggc ggaacaatta tttagctttg accggccaga taagcgagta attgctgaaa
      541 agccactccc cggtgggaga tctggttctt gactgccggc gagagctggg cggcggtaag
      601 cgtgtggccc tcgggcacaa aaatggggtc gaaaccgaag ccatgctcgc ctttctcctg
      661 gaaggctatt tctcccggcc attctacttg tttgctgaag agcacctgcc cgtcggcgga
      721 aaccagggcc agggcgcaga caaaggcagc ctggcgctta ccggccggca catcggccag
      781 ttccttcagt agtttggttc gtctgtcggc gtccgtgccc ggcgcataac gggctgagta
      841 aataccgggg cgtccgttca aggcctccac agtcaaaccg gagtcatcgg ccagggagag
      901 ttggccggtc aaagaagcgg ctgctttggc tttgatgaga gcattttctt cgaaagtgct
      961 gcctgtttcc tccgggtcaa agccgggcgg tgccaggtgg cagttgagct tgaggtgaaa
     1021 ttcccgggcc atagccagga attccttcaa tttaccggca ttggtagtgc caagggtaag
     1081 ctcgatttct ttttccgtga ccgtcattct ggctttattc ttcttttttc ttgccgttgc
     1141 gccgtgcttt ttgctcttcg ccttgtcttg gtgccggtct tggcgttact tgtcccttgc
     1201 ccccggactt agccttgcta tcggcactcg gcccggcaat gctgcgtttt ctgtggtctt
     1261 tgcggtagac gcggataacg tccgagatct gagaaatcgc ttgcgacact cttgccaact
     1321 gctcgatgtc gttcacgtcg aggaggagga agatagttgc tgttttcttg tcgcggtgag
     1381 tttcgacccg gaggtcccgg agatttactt tgtgatcgga aactttcttg agaatatcgc
     1441 cggcgatgcc cactctatcg aggcattcca cttgcagccc ggctggataa ttggtgtttc
     1501 gttctttcga ccaatccacg gccatgcgtc gattttcttc gatcttgagc agattattgc
     1561 agtcgattct gtgcacggca atgccactac ccctggtgac gacgccgaca atcggttcgc
     1621 ccggtacggg ctggcagcat ttggccagat ggtgtaagag tccgcccaga ctggatatat
     1681 tgcttggttt ttcgttctgg ggtgtaggaa cagcgtagcc ttttttctga attttctcgg
     1741 cttgatctac ttcccggatg cgatttacca cctgattgat ggaaagatcg ccgtagccca
     1801 cagcggccag tatgtcattg gcatcggcga tgtttagccg cttgccgact tccttcagct
     1861 tatccgactt gagaaattct tccagggtgc ccctacccag ttcagcttcc agcatttgcc
     1921 ggccttgctg aatatgctct tcccggtggt gtttcttgaa ccactgtctg atgcggttct
     1981 tggcgccgtg ggtattggcg aaattgagcc agtccatctt gggctgggca tttttactgg
     2041 tgatgatctc gactatgtcg ccatttttga gcaccgaatt gagcggtacg atgcggtcat
     2101 tgatgcgcgc accaatacat ctgtggccga cat
//
LOCUS       DAOQNZ010000727         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33491_length_2130_cov_4.285301, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000727 DAOQNZ010000000
VERSION     DAOQNZ010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33491_length_2130_cov_4.285301"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1822
                     /locus_tag="PKW73_12630"
     CDS             <1..1822
                     /locus_tag="PKW73_12630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74182.1"
                     /translation="ESSNFVLSVTWETGGKGGEATWLLMAEEMGDNVVLWSFRSRDAG
                     VIHKLMEEAIQALDPTNQPEDENAGQVQTVVKPGTQIDHYEIVEEIGCGGMGVVYRGK
                     DNMFERTVAIKVLHTKLLSDPLSKKRFEQEGRATIALAHPNLISVYHYGFSSAGLPFI
                     VMEYVDGKGLDKTLEQVGHLEFGEFIEIFLQACDALGHAHERGIIHRDLKPSNLMLVN
                     LGKKERLVKIVDFGISKILPHGRFPGQDLTNAGDVVGSPLYMSPEQCKGLSLDGRSDI
                     YSLGCLMYQAITGALPFLGENALQTLSKHICDPPPKFKEIAPDLKIPPELEKIIFRTL
                     EKEPEKRYETVAELHADLEAIAPKSKGLSLTTTTLSKVTSTATAGSVHILVETDTLPR
                     STLEAAVQIQKMLREGTITLAEAASAINRAHVTGGEIDIQKLASPREHTSSLVVDTPL
                     EAVLVEAGLISNAVWRTLLQLQAAIRSGEMTKDEAVQEFRKRHPKAPPKEAKPPEPEK
                     APIVVPTNPVDMLKQAGIITQADLDAAKKQAAEKGTKLEKVLVSMGKIDTKTVLASYQ
                     CMTLLSTKKLKLEQAVIALNFCERMRVDFSEAVQELGWNI"
     gene            1810..>2130
                     /locus_tag="PKW73_12635"
     CDS             1810..>2130
                     /locus_tag="PKW73_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74183.1"
                     /translation="MEHIIRNQGGNQFISDSESESCRAIWAGFAPVLSEPVFFAAINA
                     DRFLASSAAGRVIVSKEPSWRTEETITREAALLHELSGTERLPYHLPLLLPCKDHADI
                     PYLKS"
BASE COUNT          555 a          641 c          551 g          383 t
ORIGIN      
        1 cgaatcctcc aattttgtcc tttcggtcac ctgggagaca ggcggcaagg gcggcgaagc
       61 cacctggctg ttgatggccg aggaaatggg cgacaatgtt gtcctctgga gcttccgcag
      121 ccgcgacgcc ggcgtcatcc acaagctcat ggaagaagcc atccaggctc tcgacccgac
      181 caaccagccg gaagatgaaa acgccggtca ggtgcaaacc gtggtcaaac cgggcaccca
      241 aatagaccat tacgagattg tggaagaaat aggctgcggc ggcatgggcg tggtctatcg
      301 cggcaaagac aacatgttcg agcgcacggt agccatcaaa gttctccata ccaaactcct
      361 gagcgatccc ctctccaaaa agcgcttcga acaagaaggc cgagccacta tcgcgctcgc
      421 ccaccccaat ttgatctccg tctaccacta cggtttctcc agcgccggtc tgcctttcat
      481 cgtcatggag tatgtggacg gcaagggtct cgataagacc ctcgaacaag tgggtcacct
      541 ggagttcggc gagttcatcg aaatcttcct gcaagcctgc gatgctctcg gtcatgccca
      601 cgaacggggc attatccaca gagacctgaa acccagcaat ttaatgctgg tcaacctggg
      661 caagaaagaa cgtctggtca aaatagtcga cttcggcatt tccaaaatac tgccccacgg
      721 acgtttcccg gggcaagacc tgaccaatgc cggcgatgtg gtgggcagcc ccctctacat
      781 gagccctgaa cagtgcaaag ggctttctct agacggacgt tccgacatct attccctggg
      841 ctgcctgatg taccaggcta tcaccggagc cctgcccttc ctgggcgaga acgccctgca
      901 aacactgagc aagcacatct gcgacccacc gcctaaattc aaggaaatcg caccggatct
      961 aaaaatccca cccgagcttg aaaaaatcat cttccgcact ctggaaaaag agccggaaaa
     1021 gcgctacgaa acagtggcgg agctgcatgc cgacctggaa gcgatcgcac ctaaatcgaa
     1081 gggtctgagc ctcaccacca ccactctctc caaagtgacg agcacagcca cggccggctc
     1141 cgttcatatc ctggtggaaa cagatacatt gccccgttcc accctggaag ccgccgtaca
     1201 gatccagaag atgctcaggg aaggcaccat aaccctggcg gaagcggcca gtgccatcaa
     1261 ccgggcccac gtcaccggcg gcgaaataga cattcaaaaa ctagccagcc cccgcgagca
     1321 caccagctcc ctggtggtgg atacgcctct ggaagcagtg ctggtggaag caggactgat
     1381 ttccaacgcc gtctggcgta ccctcctgca attgcaagca gcgattcgct ccggtgaaat
     1441 gactaaagac gaggcagtgc aagagtttcg caaacgccat ccaaaagcgc cgcccaagga
     1501 agcgaaaccg ccggagccag aaaaggcacc aattgtagta ccaaccaatc cagtggacat
     1561 gctcaagcaa gccggcatca tcacccaggc cgacctcgat gccgctaaga aacaggcagc
     1621 cgagaaaggc accaagctgg aaaaagtact ggtatctatg ggaaaaatcg ataccaagac
     1681 ggtgctggcc agctatcagt gcatgacctt gttatccacc aaaaagttaa agttagaaca
     1741 ggcagtaatc gctcttaatt tctgcgagcg catgcgcgta gacttctctg aagccgtgca
     1801 ggaactggga tggaacatat aatccggaac cagggcggca accaattcat aagcgacagc
     1861 gagtctgagt catgccgagc catatgggca gggtttgcac ctgttctttc cgagccggtt
     1921 ttcttcgccg ccataaacgc cgaccgtttc ctggcaagct ctgcggccgg acgcgttatc
     1981 gtgagcaaag agccctcctg gcgcacggaa gagaccatca ccagggaagc agctctgcta
     2041 catgaactga gcggcactga gaggctgcct taccacttgc cgctgctctt gccgtgcaaa
     2101 gaccatgccg acattcccta tctgaaaagc
//
LOCUS       DAOQNZ010000728         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33529_length_2129_cov_3.167310, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000728 DAOQNZ010000000
VERSION     DAOQNZ010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33529_length_2129_cov_3.167310"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PKW73_12640"
     CDS             <1..854
                     /locus_tag="PKW73_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74184.1"
                     /translation="TGSRPADSELSQVQKLIAEGSLMTARPRLERLLMQYPADGKVAL
                     TAARLYQDMGLSALAIIQYERVRQLQPHLVEPLVALSRLHLENLSSALALSLAEDAAH
                     MAPADKEARLALVEALLANQYLRRAREQMESLLSLYPNDGNILHAGSMVLQACGENEK
                     ALQLLEAATRRAPARLSWQTELANLLQVQGAFREARNTLQSVIEADPFNLEALTALAH
                     LYEFDLGQYLEAREVYRRILQFLPDDTGSQAGVERCTAKQADLALWFRGMLYRTLGIS
                     AGEKARN"
     gene            complement(891..>2129)
                     /locus_tag="PKW73_12645"
     CDS             complement(891..>2129)
                     /locus_tag="PKW73_12645"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNA74185.1"
                     /translation="QGGTNAGHTVVVDGETYKFHLIPSGILYEGKTCILGPGMVIDPK
                     AFINELGALMKRGLDDTKLKVSASAHVTMPYHLALDAAEEESRGANKIGTTKRGIGPT
                     YADKCARSGIRMEDLLDKEVLKEKLEWMVPRKNVLLERMYNIPPFDAKAILEEYSGYA
                     EQIRKYVCDTSEYIFNAVQARKNILFEGAQGTLLDLDHGSYPYVTSSSPSAGGACIGA
                     GIGPTIIDRVIGVSKAYMTRVGEGPFPTEQEGEIGEMLRQTGKAWAEVGVTTGRPRRC
                     GWFDTVIGRYSVRINGLDCLAITKLDVLDEFDEIQICVAYKDKRDGSTHKEMSSIGSN
                     FKHMEPIYKSFPGWKKPTGDLRSFKELPLEAQRYLDFIADELDCPVAIVSVGPTRDKT
                     VVIEDPIHGPKRVLTKSTQQ"
BASE COUNT          425 a          575 c          625 g          504 t
ORIGIN      
        1 taaccggcag tagaccggcc gattcggagc tttcgcaggt acaaaagctc attgccgaag
       61 gttcgctgat gacggccaga cctcggttgg aaaggcttct catgcagtat cccgccgacg
      121 gaaaggtggc cctgacggcg gctcggctat atcaggatat gggtctttcc gccctggcca
      181 tcattcaata tgagcgcgtg cggcaacttc agcctcatct ggtggagccc cttgtggctc
      241 tttcccgctt gcacctggag aacctcagca gtgccctggc cctcagtctg gcggaagatg
      301 cagcccatat ggcgccggcc gataaggagg cccgcctggc gctggtggaa gccttgctgg
      361 ccaatcaata tttgcgtcgg gcccgggagc aaatggagag cttactttcg ctctatccca
      421 atgacggcaa tatcttgcac gccggctcca tggtgttgca ggcctgtggt gaaaacgaaa
      481 aagcgctgca actactggaa gcggctacac gcagggctcc cgccaggttg tcctggcaga
      541 ccgaactggc taatctcttg caagtgcagg gcgctttccg tgaggcccgc aataccctgc
      601 agtcagtcat cgaggccgat cctttcaatc tggaggccct caccgccctg gcccatctct
      661 acgaatttga tctgggtcag tatctggaag cgcgagaagt ctatcgtcgc attttgcagt
      721 tcctgcctga cgatacgggt agccaggccg gtgtagaacg ctgcacggcc aagcaggccg
      781 acctggctct ctggttccgg ggcatgctct accgcaccct tggtatttca gccggagaaa
      841 aagcccgcaa ctgagtcgcg ggctttttct ctttcgattt tgtttgcggt ctactgttgg
      901 gtagacttgg tgagaacccg cttgggtccg tgaataggat cttctataac cacggtcttg
      961 tctctggtcg gtccgactga aactatggct acggggcaat ccaattcatc ggcgatgaaa
     1021 tccaggtatc tctgcgcttc caggggcagt tccttgaagc tgcgcagatc accggtgggt
     1081 ttcttccagc ccgggaacga cttgtagatt ggttccatat gtttgaaatt ggaaccaatg
     1141 gaagacattt ccttgtgggt ggagccgtct ctcttatctt tgtaggctac gcaaatttga
     1201 atttcatcga actcgtcaag cacatcaagt ttggtgatgg ccaggcaatc gagaccgttg
     1261 atacgcaccg aatagcgacc gatcacggtg tcgaaccagc cgcaacggcg agggcgaccg
     1321 gtggtgacgc ctacttccgc ccaggctttg cctgtttggc ggagcatctc acctatctcg
     1381 ccttcttgtt cggtagggaa gggaccttcg cccacgcgtg tcatgtaggc tttggatacg
     1441 ccgatgacgc ggtcgataat ggtcggaccg ataccggctc cgatacaggc gccgcctgca
     1501 gaggggctgg aactggtgac gtagggatag gagccgtggt cgaggtcgag gagggtaccc
     1561 tgggcgcctt caaagaggat gtttttgcgg gcctgcacgg cgttgaaaat gtattcggaa
     1621 gtgtcgcaga catacttacg aatctgctcg gcatagccgc tgtactcttc cagaatggct
     1681 ttggcgtcga aaggaggaat gttgtacatg cgctccaaga gaacgttctt gcgcggtacc
     1741 atccattcca gtttttcttt caagacttcc ttatcgagca agtcttccat gcgaataccg
     1801 cttctggcgc acttatcggc ataggtggga ccgatgccgc gcttggtggt accaatcttg
     1861 ttggcgccgc ggctttcttc ttcagcggca tcaagagcca ggtggtaggg catggtcaca
     1921 tgggccgagg ctgatacttt cagcttggtg tcatcgagac cgcgcttcat gagagcgcca
     1981 agctcattta tgaaggcttt ggggtcgata accatgccgg ggccaagaat gcaggtctta
     2041 ccctcgtaga ggataccgga agggatgagg tggaacttgt aggtctcgcc atcgaccaca
     2101 accgtgtgac cggcgttggt gccaccctg
//
LOCUS       DAOQNZ010000729         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33559_length_2128_cov_3.449590, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000729 DAOQNZ010000000
VERSION     DAOQNZ010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33559_length_2128_cov_3.449590"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PKW73_12650"
     CDS             <1..825
                     /locus_tag="PKW73_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74186.1"
                     /translation="AARKQEVEVASSHAEQQRAAADLSQAQSEADSSKEDMRSAQLEL
                     AAVAGPQAARAVIQKVSGVSPTIESLAQAQPAQDVAPQGGFNVLGSVGSFFGFNKKKG
                     DEQPAPEPVKAAKADEGKKDKNAGKAKETKTKTASKENKPKKGGKAEVAQGDLEPAPS
                     KGESSSAAAQADAPAASDGISFELKGKNISARKSVLNVSIRNTSDNAFSFSPDDISVQ
                     EGNQKLSEAAMRAEFDSTSVAPNGEVKGTITIFGRPWNDRLTISLSDGQRSIHMKR"
     gene            900..>2128
                     /locus_tag="PKW73_12655"
     CDS             900..>2128
                     /locus_tag="PKW73_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74187.1"
                     /translation="MNKMSSQFKLVTLANGRARAAVALSMLFAMALLALPAFSQANTN
                     QFFTNAQGTMDDSNNSTAQTIRDMACLRYKWLAINRRYDRLQTVDSPMPATRAPRRQT
                     VPEKREEFQTDGRSARQPSQGETRTKETADSNTETKVLAYDSCFVPCGSPVGWFLQDA
                     EAWKFPKYTAYGVPCYVQPKLEAMDKVKTSRDEAIFEKATGYNPPGTGRITTFGLPIS
                     DTQFQLIDRENNNRMLELLSDPERFMWAATTTGQMMNGAAGNSIAGVAESSFDNAAAR
                     IIQGDATNSLINLANENSGTGPNLGAYYRRVSDAVRQVQIMYHQVYVPLAILFLLPGA
                     VISQAKSIVAGGFALRAQEAQSPFDGLLRSFVAVFLIPATQLIVSYAIDVGNSMAYSV
                     YDPWVNIDRVKEWTHNLS"
BASE COUNT          557 a          594 c          557 g          420 t
ORIGIN      
        1 gctgccagaa aacaggaagt tgaagtggct tcgtcccatg ccgagcagca gagagcggct
       61 gccgatctgt cgcaagcaca gagcgaagcg gattcctcca aagaggatat gcgctcagcc
      121 cagcttgaat tggcggcagt agcgggcccg caagccgcac gtgccgtaat ccagaaagtc
      181 tccggtgtgt ctcccactat cgaatctctg gctcaagctc agccagcgca agacgtcgct
      241 cctcaaggcg gattcaacgt gctgggctcg gtaggcagct tcttcggctt caacaagaaa
      301 aagggtgacg agcaaccggc gccagagccg gtcaaagccg ccaaggccga cgaaggcaag
      361 aaagataaga acgccggcaa agcaaaggaa accaagacta aaacagcgag taaagaaaac
      421 aagcctaaga aaggcggcaa ggccgaagta gcccaaggcg atctcgagcc cgctccgtcc
      481 aaaggtgagt catccagcgc cgcagcacag gcagacgctc cggcagcatc ggacggcatc
      541 tcatttgaac tcaaaggcaa aaacatcagc gcccgcaaga gcgttctcaa tgtttctatt
      601 cgcaacacat ccgacaatgc tttcagtttc agccccgatg acatatcagt ccaggagggt
      661 aatcagaagc tttcggaagc agcgatgcgt gccgaattcg actccacaag cgtagcgcca
      721 aatggagaag taaaaggcac catcacgatt ttcggtagac cctggaatga caggttgacc
      781 atatctctct ctgacggaca acggagcata cacatgaaac gttgaccatt gaagatgaag
      841 cgtttgcaga tcctagaccg cgtgaaatta ctagaccaac gctcaatcag gtagttttga
      901 tgaataagat gtcgagccaa ttcaaactgg ttacactggc aaatggccgg gcgcgcgcag
      961 cagtcgcgct ctcgatgctg ttcgccatgg cgctattagc cctgccggca ttctcgcagg
     1021 ctaatacgaa ccagttcttc acgaacgccc agggcaccat ggacgattcc aataactcga
     1081 ctgcgcaaac cattcgagac atggcttgct tgcgctacaa gtggctggca atcaaccgaa
     1141 gatacgaccg cctgcagacg gtcgacagtc cgatgcctgc taccagagcg cctagaaggc
     1201 agactgttcc ggaaaagaga gaagagtttc agacagacgg acgttcggca agacaaccct
     1261 cacagggcga aacacgtacg aaagagacag ccgattccaa tactgagaca aaagtccttg
     1321 cttatgactc ctgcttcgtg ccctgcggca gcccggtggg ctggttccta caggatgctg
     1381 aagcatggaa attcccgaaa tatacggctt atggtgtgcc ttgctatgtc caacccaagc
     1441 tcgaggcgat ggacaaagta aagacttcca gagacgaggc tatcttcgaa aaagcgactg
     1501 gctataatcc gccgggaacc ggtcgtatca ctacattcgg cttgccgatc tcggatacgc
     1561 aattccagct gattgaccgc gaaaacaata accgcatgct cgaattgctc tccgacccgg
     1621 aacgtttcat gtgggctgct acaactaccg gtcagatgat gaacggtgcg gccggcaaca
     1681 gtatcgcagg cgttgccgaa agctcctttg acaatgcggc tgcccggatc atccagggtg
     1741 acgcaaccaa ttctttgatc aacctggcta acgaaaattc aggaaccggt cccaacctcg
     1801 gcgcttacta tcgccgcgtt tcggacgctg ttcgtcaggt gcagatcatg taccaccagg
     1861 tctatgtgcc gctggctatc ctctttctct tgccgggcgc tgtgatcagc caggcaaaat
     1921 caatcgtcgc cggcggcttt gctctcagag ctcaggaagc tcagagcccc ttcgacggct
     1981 tgctgcgttc ttttgtcgct gtctttctta taccggctac tcagctcatc gtcagttatg
     2041 caattgacgt gggcaattcg atggcgtatt cggtctacga tccatgggtc aatatagatc
     2101 gcgtcaaaga gtggacgcac aacctctc
//
LOCUS       DAOQNZ010000730         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33592_length_2127_cov_3.591216, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000730 DAOQNZ010000000
VERSION     DAOQNZ010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33592_length_2127_cov_3.591216"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /gene="msrA"
                     /locus_tag="PKW73_12660"
     CDS             <1..363
                     /gene="msrA"
                     /locus_tag="PKW73_12660"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNA74188.1"
                     /translation="VDNPHYRQVCTDTTGHAEAVLVEFDPSVVSYKELLAKFFSIHDP
                     TTLNYQGPDHGSQYRSAIFYENEEQRVAAEEAKLEAAQSGPYKGRVIVTEIVPAQTFW
                     RAEEYHQDYFKKHGAVCH"
     gene            complement(447..>2127)
                     /gene="mutL"
                     /locus_tag="PKW73_12665"
     CDS             complement(447..>2127)
                     /gene="mutL"
                     /locus_tag="PKW73_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNA74189.1"
                     /translation="MEIKELFYNTPARLKFMKKANTEFGHIEEILQSLAISYPHVAFT
                     LIKDGDTVLRTTGSGSLAQAVVQAKFFTGKEALLEVAPHEEIAAKVRGLKISGLMAKP
                     LHFRGDRKGILTIVNGRTVRCPLTYKALDYVYSDLIPRGRYPLAVVRLEIDPGMVDVN
                     IHPTKKELKYQSGNEIYVTIQRALASTLRQNSMQEAAAYHAARRDLEEALPEVQEALL
                     EVQKAQTELEKVTFAPAMRTFESAQTQARTYRQPQSTEQMDFKESLNCKVSVDHYGVV
                     ETPNQAALSTTREAAPHYPESREISREINREELPTKEAMPKYTAPQAEAASEFTAPSP
                     TQKPEETSARPEDLPAAGEKFLHLRDTAGIEYELPVGWRLMGYLHNTYIFFETPEGLE
                     IVEQHIAHERTLYEKLLASQKTPGRLSENSQRLIISTPLDLSSEQLETLKISQEALAK
                     LGFEFSFDDGGNASVSQLPLELASFNYRPVIQKMLDDLATVDATNMDLEATKSIACQS
                     AIKNGMPLSVRDILKLVSDWLGTERNDTCPHGRPVRLKFSKEKIFEMFHPA"
BASE COUNT          416 a          536 c          614 g          561 t
ORIGIN      
        1 gtagacaatc cccattacag gcaggtttgc accgacacaa cgggacatgc cgaggcggtg
       61 ctggtggagt ttgatccgtc cgtcgtctct tataaggaac ttttggctaa gttcttttcc
      121 attcacgacc ccaccaccct taactatcag ggaccggatc atggcagtca gtaccgatcc
      181 gccatcttct acgaaaacga agagcaaaga gtggcggcgg aagaggctaa gctcgaagct
      241 gctcagagcg gaccttacaa aggcagggtg attgttactg aaatagtgcc tgctcagacc
      301 ttctggcggg cggaagagta tcatcaagat tacttcaaga agcacggtgc cgtctgccat
      361 tagggcgagg gcttttacag tgctttccag atagggcttg ggctgacttt gccttcgggg
      421 taagtttccc tggctggcga ctgtctctaa gccgggtgga acatctcaaa gattttttcc
      481 tttgagaact tgaggcgcac cggtctgccg tgggggcagg tgtcattgcg ctcggtgcca
      541 agccaatcgc tcaccagttt cagaatgtct cgtaccgaca gaggcatgcc gtttttaatg
      601 gcgctctggc aggctataga tttggtggct tccagatcca tattggtggc gtctacggtg
      661 gcaagatcgt ccagcatctt ttgaatcact ggtcggtaat tgaagctggc cagctccagg
      721 ggtaactggc tcacagaggc gttgccgccg tcgtcgaaac tgaattcaaa gcccagttta
      781 gccagggctt cttgcgagat tttcagggtc tccaactgtt cggaggagag gtccagcggc
      841 gttgagataa ttagccgctg tgagttttca ctgaggcgtc cgggcgtctt ctgtgaggct
      901 agaagcttct cgtagagcgt cctttcatga gcgatgtgtt gctcaactat ttccaaccct
      961 tcgggagttt cgaagaaaat ataggtgtta tgcaggtagc ccatcaagcg ccagccgacc
     1021 ggcaattcgt attcgatgcc ggcagtatcg cgcagatgca gaaatttttc accggcggca
     1081 ggtaagtctt ccggcctggc ggaagtctcc tcgggctttt gggttggcga gggtgctgta
     1141 aactctgaag cagcctcggc ctgtggcgcc gtatatttcg gcatcgcctc ttttgtcggc
     1201 aactcttctc ggttaatttc cctgctaatt tcccggcttt caggatagtg gggggcggcc
     1261 tcccttgtgg tagaaagagc cgcctggttt ggggtttcta ccacgccgta gtggtccaca
     1321 cttactttgc agttgagact ttctttgaag tccatctgct cggtgctctg gggctggcgg
     1381 taggtgcggg cttgtgtctg cgccgactca aatgttctca tggccggtgc gaaggtgact
     1441 ttctcaagct cggtctgagc cttctgtact tccagcaatg cttcttgcac ttccggcaag
     1501 gcttcttcta gatcgcgccg ggcggcatga taagccgctg cttcctgcat gctgttctgg
     1561 cgcaaggtgg aagcgagcgc cctctggatg gtcacgtaga tttcgttgcc gctttgatat
     1621 ttcagctctt ttttggtggg atgtatattc acgtccacca tgcccgggtc tatctccagg
     1681 cgcaccacgg cgagcggata gcgaccccgg ggaattaagt cggagtaaac atagtcgagg
     1741 gctttgtagg tgagggggca gcgcaccgtg cgtccattga cgatggtcaa aatgcctttg
     1801 cgatcacccc ggaaatgtag gggcttggcc atcagtccgc taattttcaa gcctctgact
     1861 ttggcggcaa tctcttcatg gggcgccact tccaaaaggg cttccttgcc tgtgaagaat
     1921 ttggcttgca ctaccgcttg ggcgaggctg ccactaccgg tggttctaag cacggtgtcg
     1981 ccgtctttga ttaaggtgaa ggccacatgg gggtagctga tggccaggct ctgaagaatt
     2041 tcctctatat ggccgaattc cgtattggcc tttttcataa acttgaggcg ggccggcgta
     2101 ttgtagaaaa gctctttgat ctccata
//
LOCUS       DAOQNZ010000731         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33647_length_2125_cov_3.464734, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000731 DAOQNZ010000000
VERSION     DAOQNZ010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33647_length_2125_cov_3.464734"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKW73_12670"
     CDS             <1..221
                     /locus_tag="PKW73_12670"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74190.1"
                     /translation="RAGIKTVIFPRTNEKDLQDLPDYVKDKVELVPVAHLDEVFAIVF
                     KGAAKSPKPSGKARAVPSTRRSALGRVK"
     gene            227..1132
                     /locus_tag="PKW73_12675"
     CDS             227..1132
                     /locus_tag="PKW73_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74191.1"
                     /translation="MLNSDGAKLVLPISVRGLALLLLSGPCLAVLFIGLENLPGAGLS
                     PVLAAESKIKLQMQKPQFDSLAAHSKTVKPVPTFLRGGLKITDAELESMLSVEPANDW
                     FKIPSWLAGKWKYYEGEVLSFQDLRTGSKFSEFFVSHEDTTRDWGYQRDGAGAYWQFQ
                     DSPSSVQTRKDEKIILYTRNKNEPLSLTEDELVCRMFYTYSTLEPVTLKVLKTYQSEN
                     IVVFRKAGDDEIRRDMSSKIFDEDGVPLSLSTSYSLARRMTPFNPINSVRGLNTKALF
                     RKFLTAHGMKDLLPAPGQDEDFEIW"
     gene            complement(1932..2087)
                     /locus_tag="PKW73_12680"
                     /pseudo
     CDS             complement(1932..2087)
                     /locus_tag="PKW73_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
BASE COUNT          467 a          541 c          540 g          577 t
ORIGIN      
        1 tgagagccgg cattaagact gtcatcttcc cccgcaccaa cgagaaagat ctgcaagatc
       61 tccccgatta cgtaaaagac aaagtagagc tggtgccggt ggcccatctc gatgaggtct
      121 tcgctatcgt cttcaagggt gcagccaagt cgcccaagcc tagcggtaaa gcgcgcgccg
      181 tgccgtcaac caggcgcagt gcgcttgggc gcgtaaagta aagaacatgt tgaattccga
      241 cggggcgaaa ttggtcttgc cgatttccgt ccgcgggctt gctttgcttt tgctttcagg
      301 cccttgcctg gctgttctgt tcattggttt agaaaatctt ccgggagcgg gcctgtcccc
      361 tgttcttgct gccgagagca agatcaagtt gcagatgcaa aagcctcagt tcgatagtct
      421 tgccgcccac agcaagaccg tgaagccggt accgactttt ctccgtggtg ggctcaaaat
      481 taccgatgcg gaactggaga gcatgctctc ggtagagcct gccaatgact ggttcaaaat
      541 accctcctgg cttgccggta agtggaagta ctatgaaggt gaagtcttga gctttcagga
      601 cctgcgcacc ggcagcaagt tcagcgagtt ttttgtcagt cacgaagata ccaccagaga
      661 ctggggctat cagcgggacg gcgccggtgc ctactggcag tttcaagatt cgcccagttc
      721 ggtgcaaacc cgcaaagacg agaagataat tctctacacc agaaacaaga atgagcccct
      781 ctcgcttacg gaggatgagc ttgtctgccg tatgttctat acctattcaa ccctggagcc
      841 ggtcacttta aaagtgctca agacctatca gagtgaaaat atcgtggtct ttcgtaaagc
      901 cggtgatgac gagattcgcc gcgatatgtc ctctaagatt tttgatgaag acggcgtgcc
      961 gctttctttg agcacttctt atagtctggc ccggcgcatg acgcccttta atcccatcaa
     1021 ttccgtcagg ggactaaaca caaaagccct cttccggaag ttccttaccg ctcacggtat
     1081 gaaagatctc ttgccggcgc cggggcagga tgaagatttt gagatctggt gagtctgccc
     1141 ctacgattct ccctcactat tcccgtgaaa aatgaggcgc acaagaaatt cgccataatt
     1201 tcgctcatta gtgatgcaaa gttcgcattt tttgagtaat aagcgaaatc ccgcatattt
     1261 tcgctcatta gtgctgcaaa gttcgcgttt ttggagtaat aagcgaaatt ccacataatt
     1321 tcgcctgcgc ctctattttt acttgagggg gtagggtgct ctcaagttgg ttcccctttg
     1381 tcttcttccg gaccgacgat tgtttctgtc agagaatcaa gcaggtagtc ggcttcaaaa
     1441 gtgcgattga gcttgatgta gagtttgatc agcctggttg tggctttgaa aacggcagtc
     1501 ttgtcgaatt gatcatcttt cgaggcaatc tccagatacc attccagaac gccggccgct
     1561 aaatggtact ggtcatttgc ataataaaga ttcgccagct tgcgcagggg gctgagcaat
     1621 tcggggttgc cgttgcccag gcactgctct ttcaggcaga gcagtgctcg caagataggc
     1681 tctgcctcgt atgtgatttt attttgtaag tcgtagtcta tcagtgtttc cagggcggtg
     1741 gcagcgcttt ctacaagttt atgtcgttcg ggatttgctc ccggagccgg ttcaccgcgc
     1801 cagtcgtctt tcgtcaacca ctcgatgtag cgcaggtcgg cactgaagaa agatacggca
     1861 gagccttccg gctcgtccag gttctttctt cttcctcttc ccttctgtgt cccggcagcg
     1921 gtctcgatat tttcttcatc caatatttca ttacagagtc cgacgcattc gttgcaaatg
     1981 tagacacccc gaccggcgat gattttgctt acctgatctt gcgatttgcc gcagaatgag
     2041 cactttagct gattgtccga agtctgcttt gattcattct tgggcatcga cgttatctcc
     2101 cttgagttgc aatgccttgt tgcag
//
LOCUS       DAOQNZ010000732         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33666_length_2124_cov_5.499275, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000732 DAOQNZ010000000
VERSION     DAOQNZ010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33666_length_2124_cov_5.499275"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..754)
                     /locus_tag="PKW73_12685"
     CDS             complement(308..754)
                     /locus_tag="PKW73_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74192.1"
                     /translation="MISANHRHEFALKDVLLPRKQRRNMAASGFMALFGGGRSQSGAK
                     SQPEREPVPMVFTVEIRTGSSEGPIEGSYHYRLLPNADFARRLEGFLGTRTPEVERPI
                     FSPDARPASQIILGLPRCPSCGDAISDPIVGCENAECPTHQSDQDK"
     gene            complement(1121..1423)
                     /locus_tag="PKW73_12690"
     CDS             complement(1121..1423)
                     /locus_tag="PKW73_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74193.1"
                     /translation="MKDTKDSKRVSGPVMVLCLFLLSWPLIGWAAWTYAHEYAATVVM
                     PWLMQVINQSGEVATYALGTGLAVAGSFVAAILVFVLLLLVSIGWYRLKSAFTKTR"
BASE COUNT          489 a          560 c          614 g          461 t
ORIGIN      
        1 catcggagcc attgccgtaa aatccaacct tgtcgacact gtggaaggcg acgcgcaggg
       61 aggcatcggg cttagcctcg gattttgaaa gctcgggggt gccgcggtcc acaactgcct
      121 ctaaaagata cgtagctagg aatatatacc caattttcgt ctctgatttc atctttacta
      181 tactgcttaa taatcccagt cgaaaaaaaa gcaaaaaaat ggcgggctcc aaccgtctgc
      241 gccagtggca gtggttggag cccgcccagt aggggtcagg gcgaactctt attcttcttg
      301 ctcaggacta cttgtcctgg tcgctctgat gagtcgggca ctcggcgttt tcgcagccga
      361 cgatgggatc gctgatcgca tcgccgcagc tcgggcaccg gggcagacca aggatgatct
      421 gggatgccgg gcgagcgtcg ggtgagaaaa tggggcgttc gacctccggc gtcctggtgc
      481 ccaggaagcc ttccagacgc cgcgcaaagt cggcgttagg caggaggcgg tagtgatagc
      541 taccttcgat gggaccttcg gacgagccgg tgcgaatctc cacggtgaag accatgggaa
      601 ccggctcgcg ctctggctgg ctcttggcgc cggactgcga gcgaccgccg ccgaagagag
      661 ccatgaaacc ggacgccgcc atgttgcggc gctgtttgcg cggcaagagc acgtccttga
      721 gagcgaactc gtgccggtga ttggcactga tcatgctctc gaaggtattt tcgccatcaa
      781 ctgaaacgct gatatgagac gggtcttcgc agaagacaac caggcgtgcc gcttctgcat
      841 tgcagacgcg gacatggatc tcggtgccct cattgaattg ttcagcggat tgaccgtcgc
      901 aaccgctgat gatgttcagg gtcgtcatat ggtttctttt ctccgtaagt gttggaccgg
      961 ggcggacagt cgagaagcgg agcttggggc tccggttgaa ctcgaccttg cgccgacagg
     1021 gcgaaagtcc gtcccggtcg ggatttgttg cgccggttgg cgcgtttagt aacctgaatg
     1081 ggcaattgcc cgcacttatg ttctcagcaa aggggctcgc tcagcgcgtt ttggtgaaag
     1141 cgctcttgag gcgataccag ccgatgctga ccagcagcag caagacaaag acgagaattg
     1201 ctgcaacaaa ggacccggcc actgccagtc cggttccgag ggcgtatgtg gccacctcgc
     1261 cggactgatt gatgacttgc atgagccagg gcattacgac agtggccgcg tattcatgtg
     1321 cgtaggtcca ggcagcccag ccgatcaacg gccaggacag caggaacaag cacagaacca
     1381 tgaccggccc ggagactctt ttggaatctt tggtgtcttt catgactttt ataccagttg
     1441 cggaagcacc cttacccgtg agggttgttc tcggggtgac attcctgagt tcatctttcg
     1501 gcgccgcatg gcacgaaggc aagctccgcg aaaagcagca ccattaacgg gaaacgtcca
     1561 tactgccgcg aaggcagaga ctcctttggt ttaaacgttt cccggcaaca cgctttcgtc
     1621 ttttgcaccc ggcgcaaaaa cccaggccgc ttcaccagcc ggattttgcc gcaggaaaga
     1681 gcaatcgcgc atgcgtataa caggcaatac agccgtctga atcagtctct catggcttcg
     1741 gacggcttgc tgctaaaagt cagttcccgg ctcctacaaa taagccctcc cagacacagc
     1801 ttcctcaaat ccggctcagt gaatttccgc agggcggatc agcattgagg gggtgaaggg
     1861 cgtatctgtg tttttttgaa aggttaggag aatttgtttg gagaaggaaa tataaagcag
     1921 caaaatcaaa ttagaactaa gtacccgctt gcttcaatga tctctcagaa aatgtttatg
     1981 agtatatgac aaccctaagg tttccttcag cattctgcaa aggcaatcca ggtcgaggaa
     2041 tgcagcgaaa agagcgcctc ccgatagcgc tagcggcgag aggcttcgaa acatcttgca
     2101 aagaagtata tatactggga tcag
//
LOCUS       DAOQNZ010000733         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33711_length_2123_cov_3.329304, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000733 DAOQNZ010000000
VERSION     DAOQNZ010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33711_length_2123_cov_3.329304"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKW73_12695"
     CDS             complement(<1..186)
                     /locus_tag="PKW73_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74194.1"
                     /translation="MPKRTDIEKILVLGAGPITIGQACEFDYSGSQACKALKDEGYEV
                     VLVNSNPATIMTDPELAD"
     gene            492..1520
                     /locus_tag="PKW73_12700"
     CDS             492..1520
                     /locus_tag="PKW73_12700"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA74195.1"
                     /translation="MAAALATLMLPAQARPERDDNPREHAMGFLPDVPMSVWQDPDVE
                     QRGIVVAVHGLVMHGRVYDTMARELAKDGLTVVAPDLRGYGRWQKLSEEESNRKSGNR
                     SEKKSEKKSEKIEYVSSLNDLKEVVSALRANSPDLPIFMVGESMGAGLSLRVAEALPG
                     QVNGLVLSSPAIKRRFYMEPEMVKDLAGLVRNPLREVDLVPYIRKFASENPTVSEEAI
                     HDPYVRKHLSCLDLLNTATFIRGNISHARGVPATVPVLVIQGDKDRMLRHEAVSALLS
                     RLKSRDTTMCWLPGKGHVLIETSHIEPNTMDTIRGWLSQHLPARSSSLISSMPAGTNL
                     FSASELSK"
     gene            complement(1510..>2123)
                     /locus_tag="PKW73_12705"
     CDS             complement(1510..>2123)
                     /locus_tag="PKW73_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNA74196.1"
                     /translation="SLTCPKRKDISCGWAGKRDIFTIAREGQALLYELNGDKPSLGEG
                     VFIAPNAVLVGRVRVEAEASIWFSCVARGDINDIVIGKQSNIQDGCLMHVTNHHALTV
                     GERVTVGHGAILHGCEIESDCLIAMGAIVLDGARVGAHSVVAAGCLVSPGTQIPPGSL
                     VMGVPGKVLRQVNERDREMIEKGWQNYVGYRQTFLQGMREPTY"
BASE COUNT          478 a          580 c          565 g          500 t
ORIGIN      
        1 gtcggctagc tccggatcag tcatgatggt ggccgggttg gagttgacga gcactacctc
       61 gtagccttca tctttgaggg ctttgcaggc ctgggagccg gaatagtcaa attcgcaggc
      121 ctgaccaatg gtaattggtc ctgcacccag gacgaggatc ttttcaatgt cggttctttt
      181 cggcacaggt ttctccaggg tcaatttggt aagcgaaaaa ttttgatttt aaccagaaat
      241 tctttcaaac agggtttcaa agatacttac aagttggtac atacgattta tcagcgctcc
      301 acttgcctaa cagcaaatca aaacatactt tgctccttct gagctaatag cctaacgacc
      361 cagggggata gatgtcgtta agttcgtctt cgtttgtcaa aaagtgcttc aaaaagtttg
      421 gaattgcccc gggaaatttc gggacagctt caaatgccgg agccggtcgc attcttgcgg
      481 gcagtcttgt tttggcggcg gctcttgcta ccctcatgtt gccggcccag gctcgacctg
      541 aacgggatga caacccccgg gagcatgcca tgggcttcct gcccgacgtg cctatgtctg
      601 tctggcaaga ccctgatgtg gaacagcggg gcatcgtggt agccgtgcac gggcttgtca
      661 tgcatgggcg cgtatacgac accatggcca gggaactggc caaagacggg ctcacagttg
      721 tagctcccga cctgagggga tacggacgct ggcagaagct ttccgaagaa gagtccaacc
      781 ggaaatcagg caatagatcc gaaaagaaat ccgaaaagaa atccgaaaag attgaatatg
      841 tctcgagcct gaacgatttg aaagaggtgg tttcagcttt aagagctaac agtcccgacc
      901 tgcccatttt catggtgggt gaaagcatgg gcgccggact ttcgctcagg gtggcggagg
      961 cgctgcccgg gcaggtgaac ggcctggtgc tctccagccc ggccattaag cgacgcttct
     1021 acatggagcc ggagatggtc aaagacctgg ctggactggt gcgcaatccc ctgcgcgaag
     1081 tagacctggt tccttatata aggaagtttg cctccgaaaa tccaacggtg agcgaagaag
     1141 ctatacatga tccctatgtt cgtaagcatt tgagctgtct cgacctgctc aacacggcta
     1201 ctttcatcag ggggaacatc tcccatgccc ggggcgttcc ggccacggta ccggttctgg
     1261 tcatacaggg cgacaaagac cgcatgctca ggcacgaagc tgtttcagcc ttgttgtcgc
     1321 gcctcaagag tcgcgatacg acaatgtgct ggcttccagg taaggggcat gtcttgatcg
     1381 aaacttccca tatcgaaccc aacaccatgg atacgattcg cggctggctc agtcagcatt
     1441 tgcccgccag gagttcctct ctcatttcca gtatgcctgc cgggacgaat ctctttagcg
     1501 ccagtgagct cagtaagtag gttctcgcat gccttgcagg aaggtctggc ggtagcccac
     1561 gtagttctgc cagccttttt caatcatttc ccggtcccgt tcattcacct ggcgcagtac
     1621 cttgcccgga acacccatta ccagggagcc ggggggaatc tgtgtgcccg gtgaaaccag
     1681 gcaaccggct gccacgacgc tgtgagcccc cactcgagcg ccgtcaagta cgattgctcc
     1741 catggcgatg aggcagtcgg attctatttc gcaaccgtgc aaaatggctc catgacctac
     1801 tgttactcgc tcccccacgg tgagagcatg gtgattggtg acgtgcatca ggcagccgtc
     1861 ttgaatattg ctctgcttgc cgatgacgat gtcattgatg tcgcctcttg ccacacagga
     1921 gaaccagatg ctggcttccg cttccaccct cacacggccc accagtactg catttggggc
     1981 gatgaaaaca ccttcgccca gacttggttt atctccattt aattcataca gcaaagcctg
     2041 accctccctg gcgatagtaa agatatctct cttcccagcc cagccgcatg aaatatcctt
     2101 cctcttgggg catgtcaagc tga
//
LOCUS       DAOQNZ010000734         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33728_length_2122_cov_5.041122, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000734 DAOQNZ010000000
VERSION     DAOQNZ010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33728_length_2122_cov_5.041122"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKW73_12710"
     CDS             <1..690
                     /locus_tag="PKW73_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74197.1"
                     /translation="FPLTGFMLAKGTGITEYGAATAFMNQFSFGGFALAGALVLIASY
                     FAVNDSNLFGSVQACAQLKNWSPRRWVAILAGVGTVVAAWLSVSGAARALESITSLNC
                     VLLPTATVIMMVEWFLCRRLFKHEVFAPLDRAPQNLRWPAIVALVTGASVGILTAGII
                     PGLESFHVGICSLQAWLTGIVFYVPLRLLEERLKSTMSVSAPVPVPVTNAVSAHASSV
                     ERSPELSVRGR"
     gene            706..1254
                     /locus_tag="PKW73_12715"
     CDS             706..1254
                     /locus_tag="PKW73_12715"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA74198.1"
                     /translation="MSQGNPWKTLSQSVVYENAWIQVQEDKVLRPDGKPGIYGIVSYK
                     NKAVGVLPIDREGNVYLVGQFRYPLGFYSWEIPEGGCPFGEDTLSAAVRELKEETGLS
                     AGHYEVLGRAHLSNSVSDEEAIFYLATDLTEGLPQPEGTESLAIRRLPFTEALAMVQQ
                     GEITDALSVLAIQSYALKLRQG"
     gene            complement(1240..>2122)
                     /locus_tag="PKW73_12720"
     CDS             complement(1240..>2122)
                     /locus_tag="PKW73_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74199.1"
                     /translation="LPSWQFAAYKEPNQFGQYQDSRLKGDWSEAEVTIEVTDTNALDL
                     TILSERFHKDGSKEDLALAMVLAESLLGEEILDKWINIVVPRSRQERKSPLAAVRLGI
                     EQRQTVNIKDLFQAVTDTINQIKEALPPQPLWQYQCSKRELEHHGRKPEDWEADQGAD
                     VNYSTLTNMPKLSRAPSRRYNFYSTAFSRHNELFCYLKIKGADELRENQERRLALARA
                     VDACLHEGQVGCLTGSGITEESVILELMLHNLKPAVELLRQVAAQTGLPATSWLLFHD
                     RDYQDEWAGLKEDSPLP"
BASE COUNT          382 a          565 c          610 g          565 t
ORIGIN      
        1 ttccctctca ccggcttcat gctggctaag ggtaccggta taaccgaata cggcgctgct
       61 acggctttta tgaatcagtt tagttttggc ggctttgctc tagccggtgc cctggtcttg
      121 attgcctcct attttgccgt caatgattcc aatctcttcg gctccgtgca ggcctgtgct
      181 cagctcaaga actggtcccc aaggcgctgg gtggccattc tagccggggt cggtacggtg
      241 gtggcggcct ggctttccgt ctcgggagcg gctcgcgccc tggagtctat tacctctctt
      301 aattgtgtgc ttttgcccac tgccacagtg attatgatgg tggaatggtt tctctgccgt
      361 cgtcttttca agcatgaagt cttcgcgccc ctggacaggg cacctcaaaa cctgcgctgg
      421 ccggctattg tggcccttgt aaccggggct tccgtgggca tcctgacggc gggtataata
      481 cccggtctgg agagcttcca tgtggggatt tgcagcttgc aggcctggct gaccggcatc
      541 gttttttatg tgccattgcg ccttcttgaa gagcgcctca agagcacgat gagcgtgtct
      601 gcgcctgtgc ccgtgcctgt cactaatgcc gtgtccgctc atgcatcctc cgtggagcgt
      661 agccccgaac tatcggttcg gggtcggtaa gggagaggaa ggaaattgag tcagggaaac
      721 ccttggaaga cgctttctca gagcgtcgtt tatgaaaatg cctggattca ggtgcaggaa
      781 gataaagtgc tgcgtcccga cgggaagccg ggcatatacg gcatagtcag ttataagaac
      841 aaagccgtgg gtgtgcttcc catcgaccgg gagggcaacg tttacctggt ggggcagttc
      901 cgctatcctt tgggctttta ctcctgggaa attccggagg gcggctgtcc tttcggggag
      961 gatactctga gtgccgctgt tcgggaactg aaggaagaaa cgggtctttc ggccgggcat
     1021 tatgaggttc ttggccgggc tcatttatcc aattcggtct ccgacgagga agcaatcttt
     1081 tatcttgcta ccgaccttac cgagggtctg ccccagccgg aaggcactga gtcactggct
     1141 attagaaggc tgcctttcac ggaagctctg gctatggtgc agcagggcga aatcaccgat
     1201 gccttgagcg tgcttgccat tcaaagctat gccctcaagt taaggcaggg gtgaatcttc
     1261 tttcaaacct gcccattcgt cctggtagtc tcgatcatga aagagcagcc agcttgtggc
     1321 aggcagtccg gtctgagcag ccacttgtct aagcagttcc actgccggct tcaagttgtg
     1381 caacatcaat tccagaatga cgctctcttc tgtgatacca ctgccggtga ggcagccgac
     1441 ttgaccttcg tgcaggcagg cgtccacggc tcgcgccagt gccaggcgcc gttcttgatt
     1501 ttctctcagt tcgtccgcgc ccttgatttt tagatagcag aaaagttcat tgtgcctgga
     1561 gaacgccgtg ctgtaaaagt tgtagcgtcg gctgggggct cttgagagtt tcggcatatt
     1621 ggtcagggtc gagtaattca catcagcacc ctggtcggct tcccagtctt ccggctttct
     1681 gccgtggtgc tccagctccc ttttactgca ttggtactgc cagagcggtt ggggaggcag
     1741 cgcttctttt atctggttga tagtgtcggt gactgcctga aagagatctt tgatgttgac
     1801 agtctgtctt tgctcaattc caaggcgcac cgccgccagt ggcgacttcc tctcctggcg
     1861 cgaccttggc accactatgt ttatccattt gtccagtatt tcttcgccca ggagcgactc
     1921 ggctagaacc attgccagag ccagatcttc cttggagccg tctttgtgga agcgctcgga
     1981 gaggatggtc agatcgaggg cgttggtgtc ggtaacttct attgttactt ccgcctcaga
     2041 ccaatcgcct ttcagtcggc tgtcctgata ctgcccgaat tgattgggtt ctttgtaggc
     2101 ggcaaactgc cagctcggca ag
//
LOCUS       DAOQNZ010000735         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33761_length_2121_cov_3.851888, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000735 DAOQNZ010000000
VERSION     DAOQNZ010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33761_length_2121_cov_3.851888"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKW73_12725"
     CDS             complement(<1..405)
                     /locus_tag="PKW73_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74200.1"
                     /translation="MSLEPDKSSTVEESLAMPPLSSETKPAERRFLSLFILCFLGAAL
                     ILSFSETPLFSYEKGRKIRGYFAPFGMRQFWRLFGPDVRTYNFYTLVIIEFADGTMKL
                     RELPRMEKLSVWQRFVHEKERNVFVQTLPGPPN"
     gene            562..1257
                     /locus_tag="PKW73_12730"
     CDS             562..1257
                     /locus_tag="PKW73_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74201.1"
                     /translation="MTTPSYNRGIAFLKKMEERAKEMSKVMPLDSTRSAPGPKVRSTS
                     EGERTVISLFLATFFVVVGAWLIPDKAAPDKWWVTQYTWPVMCSTGWVQCWSLFSPDV
                     REVNVHCNALIRFQDGTVKIYEFPRAQKMDLLTKFKREKLRKLFIDNMAWPQGEAFWP
                     SIARYLARANYDPSNPPDTVTLIVNSHAMPPPDPKHWSYRDQLPEHTEKNILITYKVK
                     PQDFLMDIPKLGQ"
     gene            1279..>2121
                     /locus_tag="PKW73_12735"
     CDS             1279..>2121
                     /locus_tag="PKW73_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74202.1"
                     /translation="MQAAASASETKSQRRPRWIDKWDQFWFKPQTPLPMAIFRILFGL
                     VLLENLLVHLWPDFDIYYSKHNLIPIQDMIAGYWHSDHMFDVLLMMPNENPYIYGTFY
                     VLTAATVFMTLGLFTRLSSWLVFLLLMSFGHHFELNQNAGDNYMRIAAMCIAFSSAGD
                     ALSLDRLFKTLKQDWRVTGFHPIYTAPWAQRLLQLQLMIAYFHTWLCKIEGAHWNDGT
                     AVYYAVRYDDVIRFPMPHFLDQLWFYQLSTWGTLVIEFIIWNLIWWRPARYWVILTGV
                     LLHLG"
BASE COUNT          519 a          572 c          511 g          519 t
ORIGIN      
        1 gttgggcggc cccggtagag tttgcacaaa aacatttcgt tccttctcat gcacaaatct
       61 ctgccagacg gaaagcttct ccatgcgcgg taactcgcgc aacttcatgg taccgtcggc
      121 gaactctatt attacaaggg tgtagaaatt ataggtgcgc acatccgggc caaacaggcg
      181 ccagaactgg cgcatgccaa agggagcaaa atagccgcgt atctttcttc ccttttcata
      241 actgaacagg ggtgtttctg aaaagctcaa gatgagcgcc gctccaagga agcagaggat
      301 aaacaggctc aagaagcgac gctcggccgg ttttgtctca ctggagagag gcggcatggc
      361 cagtgactct tccacagtgc tacttttatc tggctctaaa gacatagttg gctcgcacag
      421 cttgaagacg gcaaccggtt tcgcaaaata taccagtttc aaagcctatt ctaaacttga
      481 ttactaaagg catacatcaa ggtttcgaag caatatattc ttgctgaatc tgggtaggaa
      541 attgaggtaa gatcaaggca tttgacgacc ccatcataca accggggcat agcgtttctc
      601 aagaagatgg aagagagagc caaagagatg tccaaggtaa tgcccctcga tagcactaga
      661 tctgccccgg ggccaaaggt acgctcaact tccgaaggag agcgcactgt tatcagcctc
      721 tttctggcta ccttttttgt ggtggtgggg gcctggttga tacccgacaa agcagcgccc
      781 gacaagtggt gggtgaccca gtacacctgg ccggtcatgt gctccaccgg ctgggtgcag
      841 tgctggagct tgttttcccc tgatgtgcgg gaagtgaatg tgcactgcaa cgccttgatt
      901 cgcttccagg acggcactgt caaaatttac gagtttcccc gggctcagaa gatggacctc
      961 ttaaccaagt tcaagcggga gaaactacgc aaactcttca tagacaacat ggcctggccc
     1021 cagggtgaag ccttctggcc aagcatcgcc cgctatctgg ccagagccaa ctatgacccg
     1081 agcaatccac cagatacggt gacactgata gtcaattccc atgctatgcc gcctccggat
     1141 cccaaacact ggtcctacag agatcagttg ccggagcaca cggaaaagaa catcctcatc
     1201 acctacaagg tgaagccgca agattttctc atggatatcc ccaaactagg tcagtaaaga
     1261 accaggaaat catagctaat gcaagcagcc gcaagcgcaa gtgaaacaaa atctcaaagg
     1321 agaccacgct ggatcgacaa gtgggaccag ttctggttta agccgcaaac tcctctgccc
     1381 atggccattt ttcgcattct atttggtctt gtgcttttag agaatttgct ggtgcacctc
     1441 tggccggact ttgacattta ctattccaaa cataatctca tccccattca agacatgatt
     1501 gcgggctact ggcactcaga ccatatgttc gatgtgctct tgatgatgcc caatgaaaat
     1561 ccctacatat acggtacctt ctatgtgctg acggcagcca cggtctttat gacccttggt
     1621 ctattcacca ggctcagtag ttggcttgtc tttttgctgc tcatgtcctt cggccatcac
     1681 ttcgagctga accagaacgc cggcgacaat tacatgcgta ttgcggccat gtgtatcgcc
     1741 ttcagcagcg ccggtgatgc cctctccctc gatcgactct tcaagactct taaacaagac
     1801 tggcgggtga ccggcttcca cccgatttat acagccccct gggctcagcg gcttttgcaa
     1861 ttgcagttga tgatcgccta ctttcacacc tggctctgca aaatagaagg ggcccactgg
     1921 aacgacggta cggcagttta ctacgctgtg cgctatgacg acgtcattcg ttttcccatg
     1981 cctcattttc ttgaccagct ctggttttat cagctttcca cctggggcac cctggtcatc
     2041 gaatttatca tctggaacct tatatggtgg cgtcctgctc gctactgggt gattctcacc
     2101 ggcgtacttt tgcacctggg c
//
LOCUS       DAOQNZ010000736         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33775_length_2121_cov_2.880445, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000736 DAOQNZ010000000
VERSION     DAOQNZ010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33775_length_2121_cov_2.880445"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..812)
                     /locus_tag="PKW73_12740"
     CDS             complement(306..812)
                     /locus_tag="PKW73_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74203.1"
                     /translation="MFNSSYQNLSFRQQIISPSQSYPCPRCSTGLIEPHGLSECLNCS
                     SCERSFVALRGGRLLTPANRLGFKIAPTFWWDGLRWHWAGTTASSRQLLTLVFLFFLP
                     IVASHIAFSLNLFQDRPEWLSPALASAVIGLFSIQTIYFLCWDFDFVARQKDKEKRTP
                     LPDRPYQN"
     gene            complement(1030..1881)
                     /locus_tag="PKW73_12745"
     CDS             complement(1030..1881)
                     /locus_tag="PKW73_12745"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNA74204.1"
                     /translation="MKAYISVDLEGINGILHSSQTQPNEPGYGRAVRLLHEETNAVIE
                     GLLEGGVKEIVVNDSHWDARNLHIEELINHPGIFLRSGWQKPFSMVSGLNEAERRPDF
                     AVFLGYHARAGHARGVLSHTYRAQIFFEVRLDGKPVGETGLNAALAGHFGVPVAMLSG
                     DDTVIEEARDWLGDIAWAQVKEAVSRYSAVFPCQKESLGKLRETARTAALNKKHWQLF
                     QAKQPSSIEITMFDPSMADAAELIPGIERLSARDIAIKHSDYSVAFRLMLAVGALGAS
                     RRDPYFS"
BASE COUNT          452 a          546 c          592 g          531 t
ORIGIN      
        1 ggtttgccgt cggaggcaca ggctgccccc tcttcttcct ggctgattga attgttctcg
       61 acgtctatgc tgcaccgcct gcttggctta ttggtcactt atgccgtttg gttcaggtta
      121 ggattattcc ataatactga ttccataatt ctgaaggaaa agtgacgggg cttggctcgg
      181 ggctggtgtc tttcactgtt gccggcttca gcggcaaaaa agaccccacg gtccggcaat
      241 tcaaccagcg tccgtggggt cgcttacttt taatttttcg gctgcactcg gtagatttag
      301 ccgtctcagt tctggtaggg gcgatccggt agcggggtgc gcttttcttt gtccttctgc
      361 ctggctacaa agtcgaagtc ccagcacagg aaatagatag tctgaattga gaatagtccg
      421 atcaccgctg aggccagggc cggagagagc cactcggggc gatcctggaa gagattgagg
      481 gagaaggcga tgtgactggc cacgatgggc aggaagaaga gaaaaaccag ggtgagaagc
      541 tggcggctgc tggctgtggt gccggcccag tgccatctca agccgtccca ccagaaagta
      601 ggggcaatct tgaagcccag gcggttggcc ggtgtgagta aacgtccgcc tctcagagcc
      661 acaaatgaac gctcacagga gctacagttg aggcactcac tcagaccatg gggttctatg
      721 agcccggtac tgcaacgggg gcaggggtaa gactggctcg ggctgatgat ttgttgtctg
      781 aatgaaaggt tctggtagga agaattgaac atctcttgct tctccgcgaa agctgtagag
      841 ggacgtgaca gagccataca gctctgtgcc gcagcaaaag agactcaatg ccccagtgcc
      901 gttgcgttgt cctgaaaatc tgctgtactt gtttgatacc cgctattcaa ctcctactca
      961 acaagtcaag aggaagattt agcaaattta ataaagctcg agattctcag tgcttgccgc
     1021 tctttgccct tagctgaagt aggggtctcg gcggcttgca ccgagggcgc cgacggccag
     1081 catgaggcga aaggcaaccg agtagtcgct gtgtttgatg gcgatatccc gggccgacag
     1141 tcgttcgatg cctggaatca gctctgcggc gtcggccatg gaggggtcga acatggtgat
     1201 ttctatgcta gagggttgct tcgcctggaa gagctgccag tgttttttgt tcagggccgc
     1261 tgtccgggct gtttctctta atttgcccag gctttccttc tggcagggaa aaactgccga
     1321 atatctcgaa actgcctctt ttacctgggc ccaggcgata tcccccagcc agtcccgggc
     1381 ttcctctata acggtgtcgt caccgctgag catggcgaca ggtactccaa agtgacctgc
     1441 cagagcggca ttgagtccgg tttctcccac cggtttgccg tccaggcgca cctcgaagaa
     1501 gatctgggcc ctgtaggtat ggctgagcac gccgcgggca tggccggccc gggcatggta
     1561 tccgagaaat acggcaaagt cgggccgcct ctccgcctcg ttcaagcccg agaccatgga
     1621 aaaaggcttt tgccagccgc ttctaagaaa aataccgggg tggttgatta actcttctat
     1681 atggagattg cgcgcgtccc agtgcgaatc gttcaccacg atttccttga cgcctccctc
     1741 tagaagtccc tcaattacgg cgttggtttc ttcatggaga agtctgacgg cccgtccgta
     1801 gccgggctca tttggttggg tctggctgct atgcaaaatt ccgtttatgc cttccagatc
     1861 tacggatata tatgctttca ctgaccactc ccttgcgctt tgaattgatt ttctaattgc
     1921 cggaatttat tccaagacta ccgacaactt gccttgaatt gcttatattg atttagcaca
     1981 aagtggtcgg aataaggtaa gcatgagagg catgtcaagt acgaagcttt gttttgtcgt
     2041 attatctggc catggctctt ttatcaatgc atccggaaaa aacactcagt ctgaccgccc
     2101 cgcccctcgc cttttccaag g
//
LOCUS       DAOQNZ010000737         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33804_length_2120_cov_3.421308, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000737 DAOQNZ010000000
VERSION     DAOQNZ010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33804_length_2120_cov_3.421308"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKW73_12750"
     CDS             complement(<1..808)
                     /locus_tag="PKW73_12750"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNA74205.1"
                     /translation="MAPTKNTTASTTLAIPLWFTILAGLAIAARITVKLPSSEQKNAI
                     AWLKPSEVASRLADPGETRELIFYDCMADWCPPCKKMERTTFQSKAIIESINKDFIPV
                     RVNFSSETGPEEAATKSLKSKLSVYYLPTIAITLRNGDLVAKDWGAINLKDLMKEAKK
                     KAGLVRAERKLSQGLAGEALTLLDAKKLSGEEPINDEIEIVVYDHTLRSLGKEDDCKR
                     LLEKNYARVEEKRKALPAALNKPVFYTSLINYLQGKESFEKLVNEAANYNS"
     gene            complement(795..1622)
                     /locus_tag="PKW73_12755"
     CDS             complement(795..1622)
                     /locus_tag="PKW73_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74206.1"
                     /translation="MKQSGAAVINPAIFSFTLYRKFENPRYLIPLVLIMFLPAFMITM
                     NHLYFGSATTVEMQKERALSMVQFFCNPSLCFILSYLFVSEAFVNNKAVADADTLSLL
                     FTRPINRFSYVLTKFLAGTTGASLILILAAIIAYCSALAFGIAAIPLDPLLLLTIILN
                     TAGSTAFFVFLHCLPGIFALSAYFVLLGCAGMGSGFSSIPNGSESNAMLDLIKNVILT
                     IHYWCGDFMPAAINLQALFSAEGLDYLELGVAVTNIFIFLYLAAQALCARELSYGAD"
     gene            complement(1619..>2120)
                     /locus_tag="PKW73_12760"
     CDS             complement(1619..>2120)
                     /locus_tag="PKW73_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74207.1"
                     /translation="TSGMDPLGFILIRKLLLKLKEEGMTIVLNSHHLLEVERVCDRVA
                     FIRRGKIEEVSSLADLNTIRQMLKVEWLPQADNDPSRQEKLAQLAEACQCPLLESFDC
                     GAKFAVATNEKAAELLAGLQKENFSVYQSTFEKKELVELFLNERATDMGAEAEPDRTE
                     QREGQK"
BASE COUNT          474 a          492 c          582 g          572 t
ORIGIN      
        1 cactattgta attggctgct tcattgacta gcttttcgaa gctctctttt ccttgtagat
       61 aattgatgag gctcgtatag aagactggtt tattcagtgc cgccggcaaa gccttgcgtt
      121 tttcctctac tcttgcataa ttcttttcca gaaggcgctt gcagtcatcc tctttgccga
      181 gagaacggag agtatggtcg tacaccacta tctctatttc atcattgatt ggttcttcac
      241 cggagagttt ttttgcatcc agtaaggtca gcgcttcacc ggccagaccc tggcttagct
      301 tcctttcggc gcgcaccagc ccggctttct ttttagcctc tttcatcagg tctttgaggt
      361 tgattgcacc ccagtctttt gccaccaggt cgccgtttcg gagggtgatg gcgatggtgg
      421 gcaggtaata gacgctcagt ttgctcttca ggctcttggt ggcggcttct tccggcccgg
      481 tctcacttga gaaatttact cgcaccggta tgaagtcttt gttgatgctt tctattatcg
      541 cctttgattg aaaagtagtt ctttccattt tcttgcaggg cggacaccaa tcggccatgc
      601 aatcgtaaaa aatgagttct ctggtttctc ccgggtcggc gaggcgactt gctacttcac
      661 ttggtttgag ccaggcgata gcgttcttct gttcgctgct tggaagtttg acggttatcc
      721 gggcggcaat ggccaatccg gccagaatgg taaaccagag tggtatggcc agagtggttg
      781 aggcagtagt attcttagtc ggcgccatag gaaagctccc gtgcgcagag tgcctgggcg
      841 gccaggtaga ggaagatgaa gatattggtg acggctacgc caagttccag gtagtcgaga
      901 ccttctgccg agaagagcgc ttgcagattg atagcggctg gcatgaagtc gccgcaccag
      961 tagtggatag tgaggatgac atttttgatc aggtccagca tggcattgct ctcgctgccg
     1021 tttgggattg aactgaagcc gctgcccatg ccggcgcagc ccagcagtac gaagtaagcg
     1081 cttaaggcga agatgcccgg caggcagtgt aagaaaacaa agaaagctgt gctgccggcc
     1141 gtgttcagga tgatagtgag gagcaagagc gggtcgagcg gaatggcggc aatgccgaaa
     1201 gcaagagcgc tgcaataggc gatgatggct gccaatatga gaatcaggct ggctcctgtt
     1261 gtgccggcta gaaattttgt gagcacgtag ctgaagcgat tgatcggcct ggtaaagagc
     1321 agcgatagtg tatcggcatc agcgacggct ttgttattca caaaagcttc gctgacgaag
     1381 aggtaggaaa gaataaagca cagactggga ttgcagaaga actgtaccat cgacagtgct
     1441 cgctcctttt gcatctcgac ggtggtggca gagccgaagt agagatggtt catagtaatc
     1501 atgaaggccg gcagaaacat gatgagcaca aggggaatca agtatctggg attctcaaac
     1561 ttccggtaga gggtgaaaga gaatatggcc ggattgatga cggctgcgcc tgattgtttc
     1621 acttttgccc ctccctttgc tctgtccgat ccggctcggc ttcagcaccc atgtctgtcg
     1681 ctctttcatt gagaaatagt tctaccagtt ctttcttctc gaaggttgac tgataaacgg
     1741 aaaaattctc tttctgcaaa ccggccagta actccgctgc tttttcattt gtggcaacgg
     1801 caaatttggc gccgcagtca aaactttcga gcaaagggca ttggcaggcc tcggctagct
     1861 gtgccagttt ttcctgcctg cttggatcgt tatcagcttg aggcagccac tctactttga
     1921 gcatctggcg aatggtattt aagtcggcca ggcttgaaac ttcttcgatt ttgcctcttc
     1981 ttataaaggc cacgcgatcg caaacgcgct ctacttcaag caggtgatgt gagttcaaga
     2041 caatggtcat gccttcttct ttcaatttca gaagaagttt tctaatgagg ataaagccga
     2101 gggggtccat gccggaagtg
//
LOCUS       DAOQNZ010000738         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33907_length_2117_cov_3.725024, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000738 DAOQNZ010000000
VERSION     DAOQNZ010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33907_length_2117_cov_3.725024"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..402)
                     /locus_tag="PKW73_12765"
     CDS             complement(235..402)
                     /locus_tag="PKW73_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74208.1"
                     /translation="MKKLDAHASQRSDSGDCEALAVVLFCLASVGGSAYLIWHFYHLL
                     EQGFRCYSPLC"
     gene            complement(1102..1569)
                     /locus_tag="PKW73_12770"
     CDS             complement(1102..1569)
                     /locus_tag="PKW73_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74209.1"
                     /translation="MNKISAVPVVELSVNLHIPVSVLARVPEKLRLVREAIRVFFKGG
                     LAHSQENDPAFTTIVLAIEDASYCLKCIIKTATALDSDVGRFTFSRQKAEILRAANSA
                     RQVLESIDSICGNFRYYPAFKETFEDAATVCAVVNALDASREEIRSVLSAYGY"
BASE COUNT          577 a          555 c          519 g          466 t
ORIGIN      
        1 ccatggcgca aagcgcggaa ccgcgcggag ccaagtcaac cgaaaccact tcaccaacag
       61 gcacgtccgg cttttccagc cattcgttca aggtggattg aagggcagcc cgactgggcg
      121 cctggaaaat gcgaacgcgg aggttttcgg gtttttcgtt gagagaactc attactactt
      181 tgaaaagcgt tgggaaaagg tgaggctcca taacctcacc accagactac tgcctcagca
      241 aagcgggctg tagcagcgga aaccctgctc gagcaggtga tagaagtgcc agatcagata
      301 cgcggagccg cccacactgg ccagacagaa gagaaccacg gcaagtgctt cgcagtctcc
      361 ggaatctgac ctttgagagg cgtgtgcgtc aagctttttc attggcgcat actccaggag
      421 ggttgagttg atgggccggc tgcctgacta cagcggtgaa gctgtaggaa agagccagga
      481 ctggtcccta gattggagtt ttaggtgggt agggattctt gaagagataa agggggagat
      541 tcaggtttat tacctagcat atattgccag tctaactaaa tgcttctaag ttacttggag
      601 cgggcgcccg gaatattaat tagctcttct gcttccctaa aagtgctccg cagccaaacc
      661 ggccgacact gcaaaaggtg caccggtaga ctttcgagac atttgcaagc gaggggaatg
      721 gcgcccatta tgaagcagcg ctaagggaat gaagcgcaac attccagagc gttacaattg
      781 gcgcgcatca gcctttagcg cccccgagag aaagcaaatc aaggcaaagc ggcgataccg
      841 tattcggtat cacctcgaag agccgatatg aaccaggcgg ttcattctgt gtcctaaaga
      901 ttgagttcgc ttgccgaaca aaccaactct ctctcactat gcccgctatt ctgccctaca
      961 aaaatggcaa aattcggtca ttcgaactgc cccaaagtta attagccacc ataaccatta
     1021 gaccggtcca ctatctttcc cccaaaagaa atccccctgg agagaaaggg cgaaaagccc
     1081 ctctctctca gtgctcaagt cttagtagcc ataggcgctc aagaccgacc gaatttcttc
     1141 cctggaggca tccagtgcat ttactacggc acagaccgtc gctgcatctt caaaagtctc
     1201 cttgaaagcg ggatagtaac ggaaatttcc acagatcgaa tcgatggact cgagcacctg
     1261 cctggccgaa ttcgccgctc tgaggatctc cgccttttgc cgcgaaaagg tgaaccttcc
     1321 cacatcgcta tcaagtgcgg tcgctgtctt aataatacat ttgagacagt agctggcgtc
     1381 ctcaattgcc agaactattg tcgtgaatgc tggatcgttt tcctgggaat gagcaagccc
     1441 ccccttgaaa aagactctga tcgcctcacg aaccagacgc agtttctctg gcactcgagc
     1501 cagcacgctg acaggaatgt ggaggttaac ggagagttca acaacgggaa ctgcagaaat
     1561 tttgttcatg aatcgcatcc tttacttaat gaggtgaatg atttggatca gcttgtcttc
     1621 cacaagcgag tcaggaacgg caacaaatca aaataggctc tgccgcgaga aaccagttct
     1681 cgagccggca agcataaatt gttaattgat ttggttccga cttaagtgag aatcctgcca
     1741 gtgcgttgtg ataagacaac tgaacactga cagtctttac ctgtcattta aaatgatgaa
     1801 accggctatt gaatgcaata tcagtgggag acttccccct gatattccag agaagctctc
     1861 tgcgatcgac tagcacacct gtcgacaacc tcaaaaccca gtaaccaggc tcgtttccgc
     1921 ccacacagaa acacgacaag ccaaagcggt gataccgtat tcgatatcac catgagagat
     1981 cttcgcaaac cttcggtcca cactttcccg acaaccgagg tcatcagccg accagaacaa
     2041 ctatcgacca gcttgcagac tattctgccc cggagatttg gcagaattcg ggcagcctgc
     2101 gttaacgaag gaagtaa
//
LOCUS       DAOQNZ010000739         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_33964_length_2115_cov_3.558738, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000739 DAOQNZ010000000
VERSION     DAOQNZ010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33964_length_2115_cov_3.558738"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PKW73_12775"
     CDS             complement(<1..812)
                     /locus_tag="PKW73_12775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74210.1"
                     /translation="MAEESFRDKLQEKLIEEVKLKVSGDSQDGASGDSRSFADSAAGE
                     RTKMTESPSDSTAGERSNLAHGPSDSMTVPGNSNGASGILPVGTVVDGKYEILALVGS
                     GGMGRVYLAKQKELGTKVALKVLHRHLLDSPNAAARFKQEARAAFNLSSRNLVSVLDF
                     GQVNQEQPYLAMQFVEGVSLQDFLKTNGPMSFSQCILLLRQVAAALEEAHSKGIVHRD
                     IKPSNILLSGGKLEEGSVKVVDFGIAKGFESNESSIEKLTSTGQLIGSPLYM"
     gene            complement(900..1862)
                     /locus_tag="PKW73_12780"
     CDS             complement(900..1862)
                     /locus_tag="PKW73_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74211.1"
                     /translation="MLSNLRFRVGPLGRIKRGAQEFQGTSRRAVNFLPGQNLSPKSRV
                     NLALSNKQPILPQLINIEAQKLSGCIKMESSRYKSRSAALIYKGRVIACVYGSKSRPE
                     QVFGPEAYGLMMSEISSVNSEVASYILADDLVESAAAMFHGGVFNPAPAEDKLESLSG
                     CMQFIHDLAAPGSIAVVDEEGHPVCFLYLSGGSLLGINSLNSLVKDKDIRSLKQYIQK
                     HQAEILANSMVTASSSIDALSFSLLGQSDPHLRPQSRLGVCSVSELDAVREAANLVGV
                     YSFKPTEAARAVRSDRFISRRDLSAKQTKIGYSVHTNFDISTGA"
     gene            1900..>2115
                     /locus_tag="PKW73_12785"
     CDS             1900..>2115
                     /locus_tag="PKW73_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74212.1"
                     /translation="MQSHVSLHLEGTSQEASANVNAPGCESEFNLYLERKRERSGACG
                     QRQKPGPIWSVVGKEHSMAENLPRSDRL"
BASE COUNT          492 a          567 c          560 g          496 t
ORIGIN      
        1 ctcatgtaga ggggactgcc aatcagttgc cctgtactgg taagcttctc gatggaagat
       61 tcgttgcttt caaaaccctt ggcgattccg aaatctacaa ctttgacgct gccctcttca
      121 agttttccac ccgaaagaag aatattggaa ggtttgatgt cgcggtgcac aatgcccttg
      181 ctgtgcgctt cttcaagagc ggcagccact tgcctcagta gcaggatgca ctggctgaaa
      241 gacatcggac cattggtctt caaaaaatcc tgcaggctga cgccttctac aaattgcatg
      301 gcaagatagg gctgttcttg attgacctgc ccaaaatcga gcaccgagac caggtttctg
      361 ctgctcaggt tgaaggcggc gcgggcttct tgcttaaatc tggcggcggc gttaggcgag
      421 tcgagcaggt gccggtgcag caccttgagc gccacctttg tgcctaactc tttctgtttt
      481 gccagataga ccctgcccat gccgccggag ccgactagag ccaggatttc atacttgccg
      541 tctactaccg tgccgacagg gagtattccg cttgctccgt tggaattgcc aggcacagtc
      601 atcgagtctg aaggaccatg cgccagattg gaacgttcac cggcggtaga gtcggaaggg
      661 ctctccgtca ttttggtccg ttcaccggcg gccgagtctg caaaactgcg gctgtcacca
      721 gaggcaccat cttgcgagtc tccggaaacc ttcagcttaa cttcttcaat cagtttttcc
      781 tggagcttat cccggaaact ttcctctgcc atctttcacc ctatgaagaa tgcgagtggc
      841 agagtttatg ttgccactcg cattccatat tatccaacct ttcgttgatc tttcagcgct
      901 tatgcccccg tgctgatatc aaaattggtg tgcacactat agccaatctt ggtttgcttg
      961 gcagagaggt ctcttcggct gatgaatcta tcggagcgca cggctctagc cgcctcggtg
     1021 ggcttgaagg agtatacccc cacaagattg gcagcttccc gcacggcatc tagctcagac
     1081 acgctgcata ctcccagcct cgattgaggg cgtaagtgag gatcactctg cccgagcagg
     1141 ctaaaagaca gggcatcgat ggaactactg gcggtgacca tggaattagc cagaatctcc
     1201 gcctgatgct tctgaatata ctgcttcagt gagcgaatat ctttgtcttt cacaagggag
     1261 ttaagggagt tgattccaag cagggagccc cctgaaagat agaggaagca aaccggatgc
     1321 ccctcctcat ctaccacagc gatggagccg ggagcagcca ggtcatgaat gaattgcatg
     1381 caacccgaga gcgactccag cttatcttca gcaggagctg gattgaacac accgccatgg
     1441 aacatggcag cggcagactc caccaggtca tcggcaagaa tgtagctagc cacctcgcta
     1501 tttaccgagc ttatctcgct catcatcagc ccataggctt ccggtccgaa gacctgctcg
     1561 gggcgcgact tgctgccgta gacacaggcg atcacccgtc ctttatatat gagcgccgcc
     1621 gagcgtgatt tgtagcgact cgactccatt ttgatgcagc ccgagagctt ctgcgcctca
     1681 atgttaatga gctgcggcaa gatgggctgc ttgttggata gtgccagatt gacgcgagac
     1741 ttgggcgaca aattctgacc tggtaagaaa ttgacggcgc ggcggctggt gccctgaaat
     1801 tcctgggcgc cgcgttttat gcgacccaaa ggtcctactc tgaaacgaag attagatagc
     1861 atgattgctc cagtatagta agagggagag aatgaatcga tgcaaagcca cgtttcactg
     1921 cacctggagg gaacctcgca ggaagcatct gcaaacgtga atgcacctgg ctgcgaaagc
     1981 gaattcaatc tatatctaga acgcaaacgc gaacgcagcg gtgcttgcgg gcaaaggcaa
     2041 aagcctggcc ctatctggtc agttgttggt aaggagcaca gcatggctga aaatttgcca
     2101 cgctccgacc ggtta
//
LOCUS       DAOQNZ010000740         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34032_length_2113_cov_3.679786, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000740 DAOQNZ010000000
VERSION     DAOQNZ010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34032_length_2113_cov_3.679786"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKW73_12790"
     CDS             complement(<1..419)
                     /locus_tag="PKW73_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNA74213.1"
                     /translation="MADDEKEEEAKEEIALTPAPECLTIRDELSALIDGQVSGSLKSQ
                     IEDHLGGCESCRHELDSLKQVQSFLAKAFSQSTEDTPDIWAKMAASVPSICDVIQEDL
                     SAYLDGELTVPAQESVNQHLKECQTCLGLFKDLNATNR"
     gene            complement(412..1041)
                     /locus_tag="PKW73_12795"
     CDS             complement(412..1041)
                     /locus_tag="PKW73_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA74214.1"
                     /translation="MATVYSQKSDRDLVLACQHREPQAFEELVKRHQRTVYSLLYQLA
                     PDWEDTSDLSQEVFIRVWRNINNLRNPASFRSWLTQIVTNLFYDELRKRPRRLPTVSM
                     DEGLDSSEDDGSTRDIPDTSLMPEEKALNKEITEVIRQAMLRLPEQFRTAIVLREVEG
                     LSYEEIAVITQTEMGTVKSRIARARTKLQELLKPYLGETKTEAEVPSDG"
     gene            1530..>2113
                     /locus_tag="PKW73_12800"
     CDS             1530..>2113
                     /locus_tag="PKW73_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNA74215.1"
                     /translation="MSKTYLFTSESVTEGHPDKVCDQISDAILDKILSQDPKGRVAAE
                     CAVSTGLVLVTGEITTTANLDLQDLVRSVITEIGYVGEKGGGFDANSCAILAAVNKQS
                     PDIAGGVGKALEQREEGSADKSDETGAGDQGMMFGFACTETPEMMPMPISLAHRIARR
                     LTEVRKNGTLGYLRPDGKTQVTKSAVLAYFRQATG"
BASE COUNT          525 a          549 c          527 g          512 t
ORIGIN      
        1 cggttggtgg cgtttaaatc tttgaataga ccaaggcagg tctggcattc cttaagatgt
       61 tgattgacgc tctcctgggc gggaacggtc aattctccgt ccaggtaagc agaaaggtct
      121 tcctgaataa cgtcacatat agaaggaaca ctggcggcca ttttggccca aatatcagga
      181 gtatcttcgg ttgactggct gaaagcctta gccaggaagc tctgcacctg cttgagactg
      241 tctaattcgt gccggcagct ttcacaaccg ccaagatggt cttctatctg gctctttaga
      301 ctgccggaca cctgcccgtc gataagagcc gaaagctcat ctcttatggt cagacactcc
      361 ggcgctgggg taagcgctat ttcttctttt gcttcttctt ctttttcgtc gtcagccatc
      421 tgaaggaacc tctgcctcgg tcttcgtctc accaagatag ggtttgagca attcttgcag
      481 tttagtcctg gcacgggcaa tacgagactt cacagtcccc atttccgtct gggttattac
      541 cgcaatttct tcgtagctca acccttccac ttctctcaag acgatagcgg tcctaaactg
      601 ttccggcaaa cgcaacattg cttgacgaat tacttcggta atctccttat ttaatgcttt
      661 ttcctccggc atcagggagg tgtcgggaat atcccgggtg gagccgtcat cttcgctact
      721 atccagacct tcatccatgg agaccgtagg caaacggcgc gggcgcttcc gcaattcatc
      781 ataaaagagg ttggtgacaa tctgggtgag ccaggaacga aaagaggcgg gattgcgcaa
      841 attattaatg ttgcgccaga cccgaataaa tacttcctga gagaggtcgg aggtgtcttc
      901 ccaatccggt gccaattgat atagcagcga atagaccgtg cgctgatggc gcttgaccaa
      961 ttcttcaaaa gcctgcggtt cccggtgctg gcaggcaaga accaaatcac ggtcactctt
     1021 ctgggaataa actgttgcca tcgaaaatcc taaaaaaggc tcagaatagc cctgtcagcc
     1081 atgaccgact cgggaccttt atatgttaac actgagcgcg ggcagcccaa ccaaattgca
     1141 gtcaaagtca gtggtcaatc ctaacaagtt ccgccaggcc agttggccca ggagtttggc
     1201 gttcttgtcc ggattcatca acccaagtgt aaaacataga tacagagtaa ccggtccggg
     1261 caagagcaag cgaacagatt tcaagagcta ttttttacta atttaggcag gttcccaaac
     1321 agtaattgcc atctacctta tacatattaa tagatgtgat ttccaggctc aaaaagtggt
     1381 agcataagcg ccggtctgct tagaagcagg catcttgcgg ctttcaccgt ttgcttacaa
     1441 gtcttgtcat gagcctcctg caggttcagg aaggcacttg agaaatggtc gccagccaag
     1501 caatgttcat ttgtcaggga gataaacctt tgtctaaaac ttacttgttc acatcggaat
     1561 ctgtcacaga gggtcatccg gataaagtct gtgaccaaat ctccgacgcc attctggaca
     1621 aaattctttc ccaggacccc aaaggtagag tagcggccga atgcgccgta tcaaccggtc
     1681 tggtccttgt caccggtgaa attacaacca ccgccaacct ggacctgcaa gacctggtgc
     1741 gctcggtgat caccgagatt ggttatgtgg gcgagaaagg cggcggcttc gacgctaatt
     1801 catgcgccat cctggctgcc gtcaataagc agtcgcctga tatcgccggc ggtgtaggca
     1861 aggcccttga acagagagaa gaaggctcag ccgataagag cgatgaaacc ggagccggcg
     1921 accagggcat gatgttcgga tttgcctgta cggaaacacc ggaaatgatg cccatgccca
     1981 tttctctcgc tcaccgtatc gcccgtcgcc tgacggaagt acgcaagaac ggcactctcg
     2041 gctacctgag accggacggt aagacacaag taaccaagag tgccgttcta gcgtacttcc
     2101 gtcaggcgac ggg
//
LOCUS       DAOQNZ010000741         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34067_length_2112_cov_3.836655, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000741 DAOQNZ010000000
VERSION     DAOQNZ010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34067_length_2112_cov_3.836655"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PKW73_12805"
     CDS             <1..1125
                     /locus_tag="PKW73_12805"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNA74216.1"
                     /translation="FLCLALMDATQLHDFKNSLLSGLAFVEGGKTLLFILVVDAALVL
                     FAAVNTAYVGCIGLCTTMAKQGNLPAFILRRWADKYPLLQGYPFVCLSFMAVCAFMIC
                     ILPGQVDNLGQVYGMAFLAVMCSFCLGVIMLRVRMPLKVERAPFKSRLTMTIRGLKLP
                     VPPIVGLVSLFACEVILLFSAEGARSLGFQILGVIIIIMSFYRMGVLEGRLRELPDLR
                     LGMGKFTNQEEIPEDLPTFVLCTGGAKARSLATMLIKLLEQEEPGPKEVIIFHAEEES
                     ARRGMMHELLQRVVSQQIAPSFRNHDVVLTVKILPETLVDGLIYLKKAHPFSKVFIGT
                     GSDPETAERYARDLESNLGVSVVNIGRPTLLERANRQTSA"
     gene            1118..>2112
                     /locus_tag="PKW73_12810"
     CDS             1118..>2112
                     /locus_tag="PKW73_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74217.1"
                     /translation="MPENDIQEHEQIAYREIHFPEEMSLGSLYHIYRTANTSGWWEDK
                     LGEAQGVLTVAKETNLHLMVAPGTDLRLLNRILPSDLQSIDFRLCDISDSDLECISHL
                     TGLRYLSLPERKITDAGLHSLRNLRYLHTLDLGGTEVSDYGLNKLKHLTELRKLYVDS
                     THVSGAGAAELNQTLPNCHIIVHSGRILTFPFEKSPGKLLVARADSSGQRTWMEVGEA
                     LGSAQVSRRAFVRLEVHDRSAFQQCDKLKPDDIQSLDYLFDEIGASDFAIINRLSGLR
                     ELRLGGLEIEKNSLNEIVHLHRLRTLQLTSCSVVGQDALKPITKLAALKELRLSH"
BASE COUNT          490 a          560 c          542 g          520 t
ORIGIN      
        1 ttcctctgtc tggctctcat ggatgccact caactccatg atttcaaaaa cagcttgctt
       61 agcggactgg cctttgtgga aggcggcaag accttgctct ttatcctggt ggtggatgcc
      121 gccctggtgc tctttgcggc cgtcaacaca gcctatgtag gctgtatcgg tctttgcacc
      181 acgatggcta aacagggaaa cctgccggcc ttcattctgc gtcgctgggc tgacaaatac
      241 cccctcttgc agggctatcc tttcgtttgt ctaagtttca tggcagtatg cgccttcatg
      301 atctgcatcc tgcccggaca ggtggataac ctcggtcagg tctacggtat ggccttcctg
      361 gcagtaatgt gctcattctg tcttggggta atcatgctga gagtgcgcat gccgctcaag
      421 gtggagcgtg ccccctttaa gagccgcctc actatgacca ttcgcggtct gaagttgccg
      481 gtgccgccca tcgtcggtct ggtcagtctc tttgcttgcg aagtaatctt gcttttctcg
      541 gcggaaggag cccgttcact gggcttccag attctgggcg tgattatcat cattatgagc
      601 ttttaccgta tgggcgtact ggaaggtcgt ttgcgtgaac taccggatct gcgcctgggc
      661 atgggcaaat ttacaaatca agaagagatt cccgaagacc tgcctacctt cgtgctctgc
      721 accggcggag ccaaggctag aagtctggcg acaatgctca ttaaacttct ggagcaagag
      781 gagcctggtc ctaaagaggt gatcattttc cacgccgaag aagagtctgc caggcgcggt
      841 atgatgcacg aattgctgca aagagtggtc tcacagcaga tagcacccag cttccgcaat
      901 catgatgtgg tgctgacggt gaaaattctg cctgaaactc tcgtagatgg tctcatctac
      961 ctcaagaaag cccatccctt ttccaaagta ttcatcggca ccggctccga tccggaaaca
     1021 gccgagcgtt atgccagaga tctggagagt aaccttggtg tgagtgtggt aaacataggt
     1081 agaccgaccc ttctggaaag agcgaacaga caaaccagtg cctgagaacg acattcaaga
     1141 gcatgagcaa atcgcttaca gggagattca tttccctgaa gaaatgagtc tcggttcgct
     1201 atatcacata tacagaaccg ccaataccag cggctggtgg gaagacaaac tgggggaagc
     1261 ccagggggtg ctgaccgtag caaaagagac caacctgcac ttaatggtcg ctcccggcac
     1321 agacttgaga ctcttaaata gaatcttgcc gtccgacctg caatccatcg actttcgcct
     1381 ttgcgacatc tcagacagcg acctggagtg catttctcac ttaaccggct tgcgttatct
     1441 atctttgccg gaaagaaaaa ttactgatgc cgggctccat tccctgcgca atttgcgtta
     1501 tctgcacacc ctagatctgg gcggcacgga agtatcggac tacgggttga acaaactcaa
     1561 gcacttaacc gaattgcgca agctctacgt tgactctact catgtgagtg gcgccggtgc
     1621 cgccgaattg aatcagaccc tgcccaattg tcatatcatc gtacactccg gtcgcattct
     1681 tactttccct tttgaaaagt cacccgggaa attactggtg gccagggccg attcttccgg
     1741 gcagcgcacc tggatggaag tgggagaagc tctcggctcc gcccaggtat caaggcgagc
     1801 ctttgttcgc cttgaggtac atgatcgctc tgcctttcaa cagtgcgaca agctcaaacc
     1861 cgatgacatt cagtctttag actatttgtt cgatgagata ggcgcaagcg atttcgccat
     1921 cataaataga ctttccgggt tgcgggagct aaggctggga ggtctggaaa ttgagaaaaa
     1981 cagcctcaat gagattgtgc acttgcacag gctccgcacc ttgcaattaa catcttgctc
     2041 tgtggttgga caggacgccc tgaagcccat cacaaaactc gccgccctca aggagttaag
     2101 gctttcacat tg
//
LOCUS       DAOQNZ010000742         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34139_length_2110_cov_3.799513, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000742 DAOQNZ010000000
VERSION     DAOQNZ010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34139_length_2110_cov_3.799513"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PKW73_12815"
     CDS             complement(<1..584)
                     /locus_tag="PKW73_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74218.1"
                     /translation="MQLTAEVKYDGYDDLPRQQEVAYANSANTYLKADASTGKHYYDL
                     NAASKGYSGPWLFGKQKVEVNVNGMRGQWIQWCPVPADIRDGVLMRGNVIYQNSDGSY
                     RISPDVYEGVVESEHGFEGGRYFVGRTQHTFYSRNLPGGQMKVVANLKIHLDQRLPYN
                     PMSGGGTGGGSGSGGGGTAAYDSAGAAAPYSSYD"
     gene            complement(585..1883)
                     /locus_tag="PKW73_12820"
     CDS             complement(585..1883)
                     /locus_tag="PKW73_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74219.1"
                     /translation="MILGLLAALSCGGQGVLAEENSPSGAAQKSAAQTGGAQKTDDSS
                     GNEARVEASAQWAALEEEAEKSFLEGRLFDAESKWTKARAVAESNPNKLLLAETLNQM
                     THLLVKQGRLAEARSNLNEALEIRRRELGEENEKTAETLGNLALIEHREGHDGLAEKH
                     YKAVLDIKKKVGSEASRAITLTNLANLYGEMKRIEEARELYGEALKLDEKVYGADHLE
                     VARDLFNIGGLNYHHNYFQEALPSFDKSLVIYEKLGDSQGQIKCHHYIGLCRARLNEH
                     DKAVAAYKKAYALHSKIKGEGHIDTLVHQLNMARSLDTAGQSQEAEQIYKGALRVADQ
                     GHNEAKLRLVECSLEYAHFLRRHGRSQEAEKQLASVLGVYESLSASDRRRLYELPRAY
                     SDLLRELKREEEADQMARKHLHVYGKSAVKPAGKKNRKVR"
BASE COUNT          460 a          635 c          510 g          505 t
ORIGIN      
        1 ctatcatagc tggaataagg cgcagcggca cccgcagaat cgtaagcagc agtaccaccg
       61 ccgccactgc cactgccgcc gccagtgccg ccgccgctca tggggttata cggcaatctc
      121 tggtcaaggt gaatcttgag attggccacc accttcattt gtccgccggg taaatttctg
      181 gaatagaaag tgtgttgggt gcgccccaca aaataacgcc cgccttcgaa accatgctcg
      241 gactcgacca ccccttcata gacatcggga ctgattcgat agctgccgtc ggagttttga
      301 taaatgacat tgccgcgcat gagcacaccg tctcgaatat cggccggcac cgggcaccac
      361 tgaatccact gcccacgcat accgtttaca ttcacttcca ccttttgctt accaaaaagc
      421 caggggccgc tatagccttt ggaggctgca tttagatcgt aataatgctt gccagtactg
      481 gcatcggcct tcaaataggt attagccgaa ttagcgtaag cgacttcttg ctgccggggc
      541 agatcgtcat agccatcgta tttgacttca gcagtaagtt gcatctagcg caccttcctg
      601 ttcttcttgc cggccggctt gacggcactt ttgccataga catgaagatg tttgcgcgcc
      661 atctgatcag cctcttcttc gcgcttcagc tctcgcaaaa ggtcactgta agcccggggc
      721 agttcataga gcctgcgccg gtccgaggca gacaaactct cataaacgcc cagaaccgaa
      781 gccaactgct tttcggcttc ctggctgcga ccatggcgac ggagaaaatg cgcgtattca
      841 aggctgcact ccaccaggcg caatttcgct tcattgtgtc cctgatcggc aacccgcaaa
      901 gcccccttgt agatctgctc ggcttcttgc gattgaccgg ctgtatcgag gctgcgagcc
      961 atattcaact gatgcacaag ggtatcgata tgaccttcgc ccttgatttt gctgtgcaag
     1021 gcataggctt ttttgtaagc cgcgaccgct ttatcgtgct cgttcaatcg agcccggcaa
     1081 agaccgatat agtgatggca tttgatctgg ccctggctat caccaagttt ttcataaata
     1141 accagcgact tatcgaagga aggaagagcc tcctgaaaat aattatgatg gtagttgagc
     1201 ccaccaatat tgaagagatc ccgggccacc tccagatggt cggcgccata gaccttctca
     1261 tccagtttga gcgcttcccc gtaaagttct cgcgcctctt cgatgcgctt catctcacca
     1321 tagagattgg ctaaattggt aagggtaatg gcgcgggaag cctcgctgcc caccttcttc
     1381 ttaatgtcaa gcacagcttt gtaatgcttc tcagccagcc cgtcatgacc ttctcgatgc
     1441 tcaatcaggg caagattgcc cagggtctcg gccgtcttct cattttcctc gcccaactct
     1501 cgacggcgta tctccagtgc ttcgttcaag ttgctgcgtg cctcggccag gcgaccctgc
     1561 ttcaccagaa gatgggtcat ctgattgagg gtttcggcca ggagcagttt attagggtta
     1621 gactccgcta ctgccctggc ctttgtccac ttactctcgg catcaaagag gcgcccctcc
     1681 aggaagctct tttcagcttc ctcctcaaga gccgcccact gggcggatgc ctctactctg
     1741 gcttcattgc cgctgctatc gtcagtcttt tgagccccgc cggtttgagc agcgctcttt
     1801 tgcgccgcac ccgaaggtga gttctcttcc gccagaaccc cctgtccgcc acaactgagc
     1861 gccgccagaa gccccaaaat catggcttgc gagggacggc ggcacctccg gggaccttga
     1921 acccaaaagg agaaccacaa ggcatttggg cttggcactc tctaaactcc cggcactgaa
     1981 gatcaaacgg tttaacttgg gggataatac aacattggta cgtaccttga gagcgccctg
     2041 tcaagaaggc tataaccccc atgcaaaaca agaaaatcct cgccgtcctg gcagttgccc
     2101 tcaccgcctc
//
LOCUS       DAOQNZ010000743         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34147_length_2110_cov_3.176642, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000743 DAOQNZ010000000
VERSION     DAOQNZ010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34147_length_2110_cov_3.176642"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2110
                     /locus_tag="PKW73_12825"
     CDS             <1..>2110
                     /locus_tag="PKW73_12825"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNA74220.1"
                     /translation="CFDEPQFKAVFALTLKVKEDLTAISNTPVQQETRADGWKTVAFK
                     ETMKMSTYIVAFLVGHFDASRVVMVDGVPLRIYAPLGKGNLTEFALEIGASALAFFNR
                     YYGISYPGEKMDMIAVPDFAFGAMENLGAVIYRENALLIDPAKASHAELERVADVVAH
                     ELAHMWFGNLTTMKWWNGIWLNEAFATFMQLVAVDNFRPQWRRWETFGVSRAAAFATD
                     GLTATRKIEYPVIKPEEANGMFDVLTYEKGASVLRMLEQYVGADKFKDGVNGYLRKHK
                     FGNTETHDLWLALAQSTEEPVQEIMDSWIFQEGYPLIDVSLTADKKGLQLSQKRFFYL
                     NDANAKDSTCFQVPLKLKVSTATGITEKRHLLSDKEETILFDGPIESVLANAGGHGFY
                     RVNYEAALLEKLEQAITASFKTEVEIKHGGSAEVSERASLTLSALERFNLVNDMFALT
                     VNGTVPLNRFLNFIKLFQLESDKNVWSIIISALTYIDRVFPERESHMATLTTELLTPT
                     FACLGYTAGAGESEQIKQLRGLALTALGTLGGKRDVQEKAAALYEAHKRGESVLDAEV
                     LSAVVAILAHTGGEERYREFEDGFKNGGSPQEQERFMNALALFRDSALLQKTLEKTLT
                     GAVRSQNAPYLVRNVMLNTSGRHVGWNFVKNNWKQIRETFPSLIITRLVEGVTGLVDE
                     KLAAEVEVFFAEHPVQEGQKT"
BASE COUNT          548 a          571 c          559 g          432 t
ORIGIN      
        1 ctgctttgac gaaccgcaat tcaaagccgt ctttgccctc acactgaaag tcaaagaaga
       61 cttgacggcc atctccaaca cgcccgtgca acaagagaca agggcagacg gctggaagac
      121 ggtggctttc aaagagacca tgaaaatgtc cacctatatt gtcgcctttc tggtgggaca
      181 ctttgatgcc agccgggtag tcatggtaga cggtgtgccg ctgcgcatct atgctccact
      241 gggtaagggc aacctgactg agttcgcact tgaaatcggg gcttccgccc tggcattttt
      301 caatcgttat tacggcatca gctacccggg cgaaaaaatg gacatgattg cagtgcccga
      361 ctttgccttt ggcgccatgg aaaatctggg cgccgtcatc taccgggaaa acgccctcct
      421 catagacccg gccaaagctt cccacgccga actggaacga gttgccgatg tggtagccca
      481 tgaactggcg catatgtggt tcggcaacct gaccactatg aaatggtgga acggcatctg
      541 gttaaatgaa gcctttgcca cctttatgca gttggtagcc gtggacaact ttcgccccca
      601 gtggcggcgc tgggagactt tcggcgtatc cagagcagca gcctttgcca ccgacggttt
      661 gaccgccacc cgcaaaatcg aatatcccgt catcaaaccg gaagaagcaa acggtatgtt
      721 cgacgtactc acctacgaga aaggcgcttc ggtgctgagg atgctggagc aatatgtggg
      781 ggcggataaa ttcaaagatg gcgtaaacgg ctatctgaga aagcataagt tcggcaacac
      841 cgaaacccac gatctctggc tggcactggc gcagagcacg gaagagccgg tgcaggaaat
      901 aatggattcc tggatctttc aggaaggcta tcctctaatt gatgtctcgc tcactgccga
      961 caagaaaggc ttgcaactga gccagaaacg cttcttctac ttgaacgacg ccaacgcaaa
     1021 agacagcacc tgctttcagg tgccgctcaa actgaaagtc agcaccgcta ccggtatcac
     1081 agaaaagcgc catcttctct cagacaaaga agaaaccata ctttttgacg gtcccataga
     1141 gtcagtactg gccaatgccg gcggtcatgg tttctatcga gtcaattatg aagccgccct
     1201 gcttgagaaa ctagagcaag ccatcaccgc ctccttcaaa acagaagtag aaatcaagca
     1261 cggcggctcc gctgaagtgt ccgagcgggc aagccttacg ctatcagccc tggagcgctt
     1321 caatctagtc aacgatatgt ttgccctgac tgtaaacggc accgtgccgt tgaaccgctt
     1381 cctcaacttc atcaaactct tccaactgga gtccgacaaa aatgtctgga gcataatcat
     1441 ctcagccctg acttacatcg accgtgtctt tcccgagcgc gaaagccata tggcgaccct
     1501 tacaaccgag cttttgacac cgaccttcgc ctgcctcggc tacactgccg gcgctgggga
     1561 aagtgagcaa ataaagcagt tgcggggtct ggcactgaca gctttgggca cactgggagg
     1621 caagagggat gtgcaagaga aggcggccgc actttatgaa gcgcacaagc gcggcgaaag
     1681 tgtactggat gccgaagtgc tcagcgccgt ggtagccatt ctggcacata ccggcggcga
     1741 ggagcgttac cgggaatttg aagatggctt caaaaatggt ggttcgcccc aggaacagga
     1801 gcgctttatg aacgccctgg cccttttccg ggatagcgcg cttctgcaaa agactctaga
     1861 aaagacgtta accggagcgg tacgcagcca aaatgcacca tatttagtgc ggaacgtcat
     1921 gctcaatacg agcggtcggc acgtaggctg gaacttcgtc aaaaacaact ggaagcaaat
     1981 tcgcgaaacc ttcccgagct tgatcatcac caggctggta gaaggtgtaa ccggcctggt
     2041 ggatgagaaa ttggccgccg aagtagaggt cttctttgcc gaacaccccg tgcaggaagg
     2101 acagaaaacc
//
LOCUS       DAOQNZ010000744         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34153_length_2110_cov_2.984915, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000744 DAOQNZ010000000
VERSION     DAOQNZ010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34153_length_2110_cov_2.984915"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..877)
                     /locus_tag="PKW73_12830"
     CDS             complement(98..877)
                     /locus_tag="PKW73_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74221.1"
                     /translation="MQETIKTVAPSLRALLEGFIDYAGLYPPAKVPLDQAMKNYESYR
                     SGQYAWMLRWFVLGAAELAALPGEAEGKFSVLCDSDEARATTIESTSVIQTTRPTYCE
                     VSLANLDQLDKVKEAKLFAKIRTGGLKPEAIPTPEAVAAFIKGCAVRKLPFKATAGLH
                     HPIRAEQALTYEENSPRAVLHGFINVLMAAAFAWHTDIDIVPILEETDATAFRFDECA
                     HWRDKTLTQAQIKEARKEFMHSVGSCSFDEPVQDLKNLGFL"
     gene            966..1655
                     /locus_tag="PKW73_12835"
     CDS             966..1655
                     /locus_tag="PKW73_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74222.1"
                     /translation="MRSNFFHAWSGLAACAVVLALSFSPAGLFVSPALAQPGATVTLK
                     SVPAKDGSKSETVTLRDQKTGPIDKGSGIFYGEGHSYALSAPSGWVLDTQAGSSQGLV
                     AVFYPRGSSWSDSPVVMYSRIVKRDGRSLEKVIRADIDYMKEASPEIKESKKDPIPYG
                     SQSKLAQLRYLSADKNGNVEAVAYMEEPDWVVFCVLSARNQEQFEKAVPALKELVSSY
                     HYLTNQVQIQK"
     gene            complement(1669..>2110)
                     /locus_tag="PKW73_12840"
     CDS             complement(1669..>2110)
                     /locus_tag="PKW73_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74223.1"
                     /translation="PGKSATEKLSTAPATPAAPLTNLIGSTLAVTIDGVGNIIDDTGR
                     LVGRVNKDTTTLPKGSLSMSGRMMTVSVNSAGQMVDSTGNFVGHILGAEPLRADPSTS
                     LAGSSGTAGNMAATETAAPQPSSSWTDEDALKDAAPKLHGPIDK"
BASE COUNT          419 a          525 c          628 g          538 t
ORIGIN      
        1 ccggggctgg ccgccggtac tgccgcattt gcggggctgg aaggattggt tgcccacagg
       61 caggcggcgc ttgctcgcct gagcggggcg gaatgagtta aaggaagccg aggttcttca
      121 gatcttgcac cggttcgtcg aaggagcatg aaccaaccga gtgcatgaat tcttttctgg
      181 cttctttgat ctgtgcttgc gtaagagttt tgtctcgcca gtgcgcacac tcatcgaagc
      241 ggaaagcagt ggcatcggtt tcctctaaga tcggcacgat atcaatatct gtgtgccagg
      301 caaaggctgc ggccatgagc acgttgatga agccgtgcag caccgcccgt ggtgaattct
      361 cttcgtaggt gagggcttgt tccgctctta taggatggtg caatccggcc gtagctttga
      421 agggaagttt cctcacggca cagcctttga tgaaggcggc aactgcctcc ggtgtcggta
      481 ttgcttccgg cttgagcccg ccggtgcgaa tcttggcaaa aagctttgct tcctttactt
      541 tgtcaagctg atcgagatta gcaaggctga cttcgcaata ggtggggcga gttgtttgaa
      601 tcacggaagt cgactcgatt gtggtggcac gagcttcgtc gctgtcgcag aggaccgaga
      661 atttcccctc ggcttccccc ggcaaagcag ccagttcggc agctcccagg acaaaccagc
      721 gcaacatcca ggcatattgc cccgagcggt aactttcgta gtttttcatg gcctggtcga
      781 ggggcacctt ggccggcggg tagagcccgg cgtagtcgat gaagccctcc agaagggccc
      841 gcagtgatgg agcgactgtc tttattgtct cttgcatgaa ttttcgactc tcccgcacac
      901 tggcatagtt gaatttggag atgttaccat gttcctgtca gtggcgctcc tgtgggagaa
      961 tcctaatgag aagcaatttt tttcatgcct ggtccggact tgccgcctgc gcggtggtgt
     1021 tggctttgtc ttttagcccg gcagggttgt ttgtttcgcc ggctttggct cagcccggtg
     1081 caacggtgac acttaagtct gtacctgcaa aagatggaag caagtcagaa acggtcactc
     1141 tccgtgatca gaagaccgga ccgattgata aaggctccgg catattctat ggagaggggc
     1201 attcttatgc cttgagtgca ccgagcggtt gggttctcga tacgcaagcc gggagttcgc
     1261 aggggctggt ggccgtcttt tatccgcgcg gctcatcttg gtcggatagc ccggttgtaa
     1321 tgtatagccg catcgtcaaa cgtgacgggc gttctcttga gaaagtaatt cgggccgata
     1381 tcgattacat gaaggaagct tcgcccgaaa tcaaagagag caagaaggac cctatcccct
     1441 atggtagcca gtcgaaactg gcccagctca ggtatttaag cgccgataag aacggcaatg
     1501 ttgaagccgt tgcttatatg gaagagcctg attgggtcgt cttctgtgtt ttatctgcgc
     1561 gcaatcagga gcagtttgaa aaagccgtgc cggccttaaa agagcttgta agctcttatc
     1621 attatttgac caatcaagtg cagattcaaa agtagtagtt agtgatgctt atttgtcgat
     1681 tggtccgtgc agtttgggcg cggcatcctt gagcgcatct tcatcggtcc agcttgaact
     1741 cggttgcggt gctgctgttt ccgttgctgc catgttgccg gccgtgccgc tgctgccggc
     1801 caggctggtg gatggatcgg cgcgcaaggg ttcagccccg agaatgtgtc cgacgaagtt
     1861 gccggtggaa tctaccattt gtccggcgct attgactgat acagtcatca tgcgtccgga
     1921 cattgagagt gagcctttcg gcagagtggt cgtatctttg ttgacccgac ctaccagtct
     1981 gccggtatcg tcaataatat ttcccacacc atctatggtg acagccaggg ttgagccgat
     2041 caaattggtg agaggcgctg ccggcgtcgc cggagcggtg ctcagtttct cggtggcgct
     2101 cttaccgggt
//
LOCUS       DAOQNZ010000745         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34195_length_2109_cov_2.732230, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000745 DAOQNZ010000000
VERSION     DAOQNZ010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34195_length_2109_cov_2.732230"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKW73_12845"
     CDS             complement(<1..809)
                     /locus_tag="PKW73_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74224.1"
                     /translation="MSLKTKNSGKTLPTCAFVTALAVFLTLNLGLAISRPLTPLKASA
                     LPAKASWECQRALRFQKDSADATPDVVLFGSSLMMIPTTCAEADFQGKIIDPVLEPYS
                     NKLSQLLKAKGRLFQRCFNFALPGAVISDHYLVSKALFKENQRPRLVVLGITLRDFMD
                     CGVDSVAATPAYNYFHRYLSEAELDAMVQAGQGGPWQNREYLVNKWLYIYGRRLELQN
                     LIETALNSSTTAALKSLNLPREGNPSLTYEELSKPRASGVSGNKNNIDALMA"
     gene            1003..>2109
                     /locus_tag="PKW73_12850"
     CDS             1003..>2109
                     /locus_tag="PKW73_12850"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74225.1"
                     /translation="MKPESGLVRVRGARQNNLKDIDLDIPRDAFVVFTGVSGSGKSSL
                     AFGTIYAEAQKRYFETVAPYARRLLEQLPAPLVKELTGLPPAVALQQRRSDANSRSTV
                     GTLTTLANSMRMLFSRAGTYPKGFVGRLDSDAFSANTPAGACPACHGIGIIHSVSERS
                     LVPNPELSIRDRAIAAWPGAWQGKNYRDILATLGYDIDRPFKDLSEADRQWILFTDEQ
                     PVVTVYAEREAHRIQRPYKGTYMSAARYVKHTFATTNSGTLRKRVEQFMDKSGCTVCQ
                     GKRLKPEALSVKFAGYDIAELSRLPLTELAAVLAAAPELASGAKSNPDQKQKAAALIV
                     HELLERIELLDELGLAYLSMERSAPSLSSGELQRL"
BASE COUNT          460 a          546 c          603 g          500 t
ORIGIN      
        1 gccatgagcg catcgatatt gtttttgttg ccgcttacgc cggaggctct gggcttggat
       61 agctcttcgt atgtgaggga ggggttgccc tcacggggca aattgaggct cttcagggcg
      121 gcagtggtag agctgttcag ggcagtctcg atgagatttt gcaattccag gcggcgacca
      181 tagatgtaga gccacttgtt caccaggtat tcgcgatttt gccagggtcc gccctgtccg
      241 gcttgcacca ttgcatcgag ttccgcttcg cttaaataac ggtgaaaata gttataggcg
      301 ggcgtggctg ccacggaatc tacgccgcag tccataaagt ctctcaaggt aatacccagc
      361 acaaccagtc ttggtctctg gttttccttg aagagcgcct tgctgaccag gtaatgatca
      421 gagatgacgg cgccgggcag ggcaaaattg aaacaacgct ggaagagccg tcctttggct
      481 ttcagcagtt gcgaaagttt gttggaatag ggctccagca ccggatcgat aatcttgccc
      541 tgaaagtctg cctcggcgca ggtggtggga atcatcatca aggacgagcc gaacagtacc
      601 acatccggtg tggcgtcggc gctgtctttc tgaaagcgca acgctctctg acattcccaa
      661 ctggcttttg ccggcagggc ggaggctttc aggggggtga ggggacggct tatggccaga
      721 cctaaattga gcgtcaaaaa gactgccagg gccgtgacaa aggcgcaggt tggcagggtt
      781 ttgccactgt ttttagtctt cagagacaag tctcatcctc tccatacagg ttgtaaggat
      841 acagcaaagc aaggggggct acttgaaatg cagctataaa tggagccatt tgagccgggg
      901 ctaagtgaga cttggttaat tttagaccgc tcagcactgc ctattctcat actattcatg
      961 gaattacggt taatgatagg tcgacttaaa gttgtgcaaa cattgaagcc agagtcggga
     1021 ttggtgcggg tgcgaggcgc caggcagaat aatctcaaag acatcgatct ggatatcccc
     1081 agagacgcct tcgtggtttt caccggtgtt tccggctccg gtaagtcatc cctggctttc
     1141 ggcaccatct acgccgaggc ccagaagcgt tacttcgaaa ccgtggcacc ctacgccagg
     1201 cgcttgctgg aacaactgcc ggcacccctt gtgaaagaac ttaccggtct gccgccggca
     1261 gtggctttgc agcaaaggcg ctcagacgcc aacagccgct ccacggttgg aactctcacc
     1321 actctggcca attccatgcg catgctcttt tcccgggccg gcacctatcc taaaggcttt
     1381 gtcgggcgcc tggattccga cgccttttcg gctaatacgc cggccggtgc ctgccccgcc
     1441 tgccacggta ttggtattat ccatagtgtc agcgagagat cgcttgtacc taatcctgaa
     1501 cttagtattc gcgacagggc cattgccgcc tggcccggcg cctggcaggg caagaattac
     1561 cgtgacattc tggctaccct gggctatgac atagaccgac ctttcaaaga tttgagcgaa
     1621 gccgaccggc agtggatctt atttaccgat gagcagccgg tggtgactgt ttatgcggag
     1681 cgagaggcgc accgtattca gagaccatat aaaggcactt atatgagcgc ggcgcgttat
     1741 gtcaagcata ctttcgctac cactaatagt ggcactctgc gcaagcgcgt agaacaattt
     1801 atggataaga gcggctgcac ggtttgccag ggaaagcggt taaaaccgga ggctctctct
     1861 gttaagtttg ccggctatga tattgccgaa ctctcccgtc tgccgttgac tgagcttgcc
     1921 gccgtactgg cggctgcgcc ggaactggct tctggcgcta agtcgaatcc ggatcaaaaa
     1981 cagaaggcgg cagccttgat tgtgcatgaa ctgctggagc gtatcgagct tttagatgag
     2041 cttggtctgg cttatttatc gatggagcgc agtgcgccca gcctttcgtc cggcgaatta
     2101 cagaggctg
//
LOCUS       DAOQNZ010000746         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34211_length_2108_cov_4.358013, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000746 DAOQNZ010000000
VERSION     DAOQNZ010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34211_length_2108_cov_4.358013"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKW73_12855"
     CDS             complement(<1..274)
                     /locus_tag="PKW73_12855"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNA74226.1"
                     /translation="MPALSNNLTARERLIVALDVDSMEEAERLMVLLKDEVGMFKIGL
                     ELFTSVGPALFELVRKHGVKVFFDGKFHDIPNTVAGACRAAVQHNVD"
     gene            complement(438..653)
                     /gene="rpmE"
                     /locus_tag="PKW73_12860"
     CDS             complement(438..653)
                     /gene="rpmE"
                     /locus_tag="PKW73_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNA74227.1"
                     /translation="MKEGIHPKYFEVVATCVCGAAVQMGSTKNQIRVEICSSCHPFYT
                     GQQKIVDTEGRVDRFVKRYKLDKAAAK"
     gene            complement(777..1346)
                     /locus_tag="PKW73_12865"
     CDS             complement(777..1346)
                     /locus_tag="PKW73_12865"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNA74228.1"
                     /translation="MIHKLPTCLLFLAFLIPGLLLSGCSRQVLQEGQVRSTSAPKFKK
                     EQLPRLSQIEIWFVKPQGEELRLEKVLRSYNGPYTTCDYLDFAMKELTKGPDGNEAQQ
                     GLSSELPRGTVLIDIRKKGEKEVSINLSRSFFQGGGIESFETRMEQLRRTVTGIVGPS
                     NKTRVYLDIEGERLTRTVGEGLEVKQPLN"
     gene            complement(1367..>2108)
                     /locus_tag="PKW73_12870"
     CDS             complement(1367..>2108)
                     /locus_tag="PKW73_12870"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HNA74229.1"
                     /translation="TVTGLNLLTEVVASQAARLAIQKKVGGKVKVKVKTFSFTDLLAG
                     KVKAVDVQVLDPKVKGFGAGEIKITSDSPFWYQYRRGKGQKTGMQTPVLMSVKAELSE
                     KQIEAALNTPEVTNSLRGLKLDLPGLGDQSLQVLKPKVDLAEERITLTAVLVVQGAAE
                     ETGVPVKVSAKPQLEGDRKILLTDLKVDSDGIVEPEKFAAFAETLLNPVIDFARLDRR
                     DHAFRLNHFKIDGEEVVGDGKLLLTPRQ"
BASE COUNT          516 a          573 c          503 g          516 t
ORIGIN      
        1 tatcgacgtt atgctgtacg gccgctcggc aggcgccagc caccgtgttg ggaatatcgt
       61 gaaacttgcc gtcaaaaaag accttcacac catgcttgcg tactagttca aagagggcgg
      121 gaccgaccga ggtaaagagt tccagaccta tcttgaacat gcccacttca tctttgagga
      181 gcaccatcaa gcgctcggct tcttccatgc tgtccacatc aagggcaacg atgagtctct
      241 cccgagcggt caaattgttg cttaaagccg gcattgtaag catcgttcct cactgcaatg
      301 aaaatatgaa tagagccata gctgacaaga tcatctgcga tagatcatct gcaaaacggc
      361 ctgacactaa aagtgccaag ccgaatgaca aattctaaat tgcgaatgtc agcaaaagcg
      421 ctgacgaatt ctgaaagtta cttggcagca gctttgtcga gcttgtatct cttgacaaag
      481 cggtctaccc taccttccgt gtcaacaatt ttttgctgac ctgtatagaa aggatggcag
      541 gaactgcaga tttctacgcg aatttgattt ttggtggaac ccatctgtac tgcagcgccg
      601 cagacacaag tcgcgacaac ttcaaagtac ttggggtgaa ttccttcctt cattacggca
      661 ccctggaaaa tctatagaca aatactcaag aaagatatca cacctgctat gtactgtagg
      721 cagatactga gcatttcgta aggtcccgta acagcagcgc cttaaatatc ttaaattcag
      781 ttgaggggct gttttacttc cagcccttcg cccacggtgc gggtgagccg ttccccttca
      841 atgtccaggt aaacccgggt cttattggaa ggtccgacga tgcctgtgac ggtgcggcgc
      901 aactgctcca tgcgcgtttc gaaagactcg atgccgccac cctggaagaa actgcgggag
      961 aggtttatgg aaacctcctt ttcacccttc ttgcggatgt ctataagtac cgtcccccgc
     1021 ggcaattcgc tggaaagtcc ctgctgagcc tcgttaccgt cgggtccttt ggtcaattcc
     1081 ttcatggcga agtcgagata gtcacaggtt gtatagggac cgttgtaaga acgcaaaacc
     1141 ttttccaggc gcagttcttc gccctgcggt ttgacaaacc agatttcaat ctggctgagc
     1201 cggggcaact gctccttttt gaatttggga gcactggtcg atctaacctg accttcttgc
     1261 agtacctgac ggctgcagcc gctcaaaagc aagcccggga taaggaaagc gagaaacagg
     1321 aggcaggtcg gaagtttatg tatcacttgt atcaaggaag gctctttcat tgccgtggtg
     1381 tcaagagaag cttgccgtca ccgaccactt cttcaccgtc aattttaaaa tgattgaggc
     1441 gaaaagcgtg gtcccgccta tccagacgcg caaaatcaat gacgggattc aagagggttt
     1501 cggcaaaggc cgcgaatttt tcgggttcca caatgccatc ggagtcgacc ttcaaatccg
     1561 tcagcaaaat cttgcgatct ccctccagtt gcggtttagc ggaaactttc accggcacgc
     1621 ctgtttcttc cgcagccccc tgcaccacca gtacggcagt aagagtaata cgctcctctg
     1681 ctaaatcaac cttcggcttc agcacctgta aagactgatc ccctagcccg ggcagatcga
     1741 gtttgagacc gcgcaaactg ttggtcactt ccggcgtgtt gagggccgct tcgatttgct
     1801 tctcggaaag ctcagccttc acactcatga gcacaggcgt ctgcataccg gtcttctgtc
     1861 ctttgccgcg tctatattga taccagaagg gactgtcgga agtaattttg atctcgccgg
     1921 caccaaaacc cttcaccttc ggatcgagca cctggacgtc cacagccttc accttaccgg
     1981 ccaacaaatc ggtaaaactg aaggtcttta ccttcacctt caccttgccg cccacctttt
     2041 tctgaatagc caggcgcgcc gcctgagagg ccacaacttc ggtcaataga ttgagaccgg
     2101 tgacggtt
//
LOCUS       DAOQNZ010000747         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34230_length_2108_cov_2.736483, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000747 DAOQNZ010000000
VERSION     DAOQNZ010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34230_length_2108_cov_2.736483"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKW73_12875"
     CDS             complement(<1..662)
                     /locus_tag="PKW73_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA74230.1"
                     /translation="MGHKHERPVEPFSDLHVEHGHVRATLLHDPTVEGLDMAIYMDGS
                     ASMSGDYAYKKKYNNLIDWFFGRNEVNLPNNVEPQVQWILEYLATKDRNGLLRVAYWA
                     CGASGKDIELVGELKGTDVQSYKFPGPNFQGKSTNLAPALKDYVNYLRKQMHLGARRG
                     CAVFVTDGEIHDEDDVKRYCNEIAKEMQKGSLPKINFILVGVGEGVNEEQMEEICHEE
                     YPG"
     gene            complement(686..>2108)
                     /locus_tag="PKW73_12880"
     CDS             complement(686..>2108)
                     /locus_tag="PKW73_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74231.1"
                     /translation="PAFNRAEDFIPLYALDNFFAGGRHLQEQEWIVSTDAAGLLRATS
                     GDQVGTLSEDIQSQIAFSEVACEGNIRLVETIDFYLFNIQLMEQAPGAPGLNDPIYLY
                     GTLFGSYPAAGGSNAICVEGQLTVTRADLLEALVQGAKYRFKASGAGADVGSTTSIHH
                     LVLPGDRDEETIRGAGIIFAYPLMPPDMMSYGAGNELLVSQYLFDILSGIKEDLEKEN
                     IKVPLRKMRLPVPNRLTVEQELEAQGYIIKGDTAVRKPNMTGGFQGFLQSVFGSGQQE
                     KLTLPEQGSLEDFISLANQVMHSLPGWPPERTKIMRSKIQTASREVRMRAKGTAALKP
                     REIKIPNQTDMQPARARSTAPSTTSSAAYSNQPPPWMQDFINKHKNNEQEGWSKLTQS
                     NLQQGKAELTNNPYKGQTFSYTSNTSYAEFNNKRQKPTWMEDFSEAGDAKADKPVKPP
                     PEEKKTGNPSDKTKKPDWMKDFE"
BASE COUNT          378 a          536 c          596 g          598 t
ORIGIN      
        1 cccggatact cctcgtgaca aatctcttcc atctgttctt cgttgacacc ctcgcctacg
       61 ccgaccagaa taaagttgat ttttggaagg ctgcctttct gcatttcctt ggcgatttca
      121 ttgcagtagc gcttgacgtc gtcttcgtcg tgaatttctc cgtcggtaac gaagacggcg
      181 cagccccgcc tggcgcccag gtgcatttgt ttacgcaggt agttgacgta atcttttaat
      241 gccggagcca gattggtcga tttgccctgg aaattgggtc cggggaactt atagctctgt
      301 acgtcggtgc ctttcagctc gcccaccaac tcgatatcct tgccgctggc gccgcaggcc
      361 cagtaagcca cgcgcaggag gccgtttctg tccttggtgg ccagatactc caatatccat
      421 tgaacctgag gttccacgtt attgggcaaa tttacttcat tgcgcccaaa gaaccagtca
      481 atcagattgt tgtacttctt cttgtaggca taatcgccgc tcatactggc tgagccgtcc
      541 atgtagatgg ccatgtccag gccttccacg gtggggtcgt gaagaagagt ggcccgcaca
      601 tgcccgtgtt ctacgtgcag gtcggagaag ggttctactg gtctttcgtg tttgtgtccc
      661 attttcttgc ctctgtttcg tggggttatt cgaagtcttt catccaatcg ggctttttcg
      721 ttttatcgct cgggtttcct gtttttttct cttccggagg aggtttgacc ggtttgtcgg
      781 cttttgcatc gccggcttcc gagaaatctt ccatccaggt gggcttttgc cgcttgttgt
      841 taaattctgc gtaggatgtg ttggaggtat agctgaaggt ttgtcctttg tatggattgt
      901 tcgtgagttc agctttgcct tgttgcaagt ttgactgggt gagtttgctc cagccctctt
      961 gctcattgtt cttatgtttg ttgatgaagt cttgcatcca gggtgggggc tgattgctgt
     1021 aagcggcact gctggtagta ctgggggcgg tgctgcgtgc tcttgcgggc tgcatatccg
     1081 tttgattggg gatctttatc tctcttggtt tgagagccgc cgtgcccttg gctctcatgc
     1141 gcacttctct ggaggctgtt tgaattttgc tgcgcattat cttggtgcgc tcggggggcc
     1201 agccgggcag agaatgcata acctgattgg cgagagagat gaaatcttcg agactgcctt
     1261 gctccggcag ggttagtttt tcctgttgac cggagccgaa gaccgattgc aggaagccct
     1321 gaaagccgcc ggtcatgttg ggtttgcgta ccgccgtatc gcctttgatg atataaccct
     1381 gggcttccag ctcttgctct actgtgagcc ggtttggcac cggcaggcgc attttgcgca
     1441 gcggcacctt tatgttttct ttctccaggt cttctttaat accgctcaaa atatcaaaca
     1501 agtactggct caccagcagt tcgttgccgg cgccgtacga catcatatcc ggtggcatga
     1561 gcggataggc gaaaatgata ccggcgccgc gaatggtttc ttcgtcccga tctcccggta
     1621 agaccagatg gtgaatgctt gtggtgctgc ccacatcggc tccggctccg gaggctttga
     1681 aacggtattt ggctccctgt accagagcct ccagcaaatc ggcgcgggtg acggtcagtt
     1741 gcccttccac gcaaatggca ttgctgccgc cggcagccgg gtaagagccg aagagggtgc
     1801 cgtagaggta gatagggtca ttgagaccgg gagcaccggg ggcctgctcc ataagttgaa
     1861 tgttaaagag ataaaagtcg atagtttcta caagtctaat gttgccctcg caggccactt
     1921 ccgagaacgc tatctgggac tgaatatctt cactgagggt gccgacctgg tcgccgctgg
     1981 tggcccggag aagtccggcg gcatcggtgg acactatcca ttcttgctct tgcaggtgtc
     2041 tgccgcctgc aaagaagttg tcgagagcgt acagcgggat gaagtcttca gcacgattga
     2101 aagccggc
//
LOCUS       DAOQNZ010000748         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34267_length_2107_cov_2.514620, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000748 DAOQNZ010000000
VERSION     DAOQNZ010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34267_length_2107_cov_2.514620"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKW73_12885"
     CDS             complement(<1..655)
                     /locus_tag="PKW73_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74232.1"
                     /translation="MTTTYAFLSSNERKSAEYKAQLAKYGRAICILPCPDEPAEQLLQ
                     IAAYLQRAQESDRYVLRERSNLFVAADWDEGLPTISSKSINGERVYNLSILDVYYLDP
                     AGALQTSTYQAKIEGSLDLTLRQTGTDSDWWDDIFVAQRSQASYQQERDLWGKTSARQ
                     QTIGLFICDHVVFKQLKDVSFKPHRPAQSVDFTVEHAGLILRHNPFVKLARLEQSAWG
                     "
     gene            complement(796..1017)
                     /locus_tag="PKW73_12890"
     CDS             complement(796..1017)
                     /locus_tag="PKW73_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74233.1"
                     /translation="MTDLPHGSETLITGIDLIAFHPLSKDARGTAGKPPVFVNTVFRS
                     GLHRLYEQILLCPASGYPGKYKKFLHFIH"
     gene            1595..1996
                     /locus_tag="PKW73_12895"
     CDS             1595..1996
                     /locus_tag="PKW73_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74234.1"
                     /translation="MIGKVMGLCLNRLICRLSSNPPFLKGLTMQLVLEPPRGGKSWIF
                     FANDLAHDEGFVAYEPCYSRIPDSERWTVNQYLCEAAEEFGIKPHIFNQCQWEGSTGW
                     EFWTDDRPKIKAVVEKVAERLGLTVDTSALG"
BASE COUNT          421 a          518 c          608 g          560 t
ORIGIN      
        1 gtccccatgc cgactgttcc agtcttgcca gtttgacgaa aggattgtgg cgcaagatca
       61 agccggcgtg ttcgaccgtg aaatctacgg actgggcggg tctatgcggt ttgaaggaga
      121 catctttcaa ttgcttgaag accacatgat cgcagatgaa gagtccgatg gtctgctgcc
      181 tggcagaggt tttgccccag agatcacgct cttgctgata gctggcctga cttctttgtg
      241 ccacaaaaat gtcgtcccac cagtcgctgt ccgtgccggt ctggcgtaga gtgaggtcca
      301 gcgaaccctc tattttggct tgataggtgg aggtttgcag ggctccggct gggtcaaggt
      361 agtagacgtc tagaatcgag aggttgtaaa cccgctcgcc atttatgctt ttactgctga
      421 tggtgggcag cccttcgtcc cagtcggcgg ccacgaagag attgcttctt tccctgagaa
      481 catatctgtc gctctcttgt gctcgctgca gataggcggc aatttgcaaa agctgctcag
      541 cgggctcgtc agggcagggc agaatacata tcgctcgtcc gtacttagcc agttgagctt
      601 tgtactcggc tgattttctc tcattactgg agagaaaggc gtaggtcgtt gtcatagtta
      661 tctcccggaa agttttcaag gcaaactctt cccattgtcg aagtcttgtt cagaatcttg
      721 ctgaagcaag gttgttcgcc aaaagggaag aaaaggtcgg ggaagactta gaaaatagtt
      781 gctgggaagc tcttgttagt ggatgaagtg taggaacttc ttgtatttac cagggtagcc
      841 ggatgccggg caaagtagga tttgctcata gagtcgatgc agcccggacc ggaagaccgt
      901 gtttacgaac acaggcggct ttccggctgt gccgcgggca tctttcgaga ggggatggaa
      961 ggcaatgaga tctatcccgg ttatcagtgt ctcgctgccg tgtggtaaat cggtcaatgg
     1021 taccttcttg gatctcaagt gctgatctgg gtgcggtact ctctgtggtg ctgtttgagc
     1081 ggcaaaaaat acctttgttt ttccggcgct cagtctagtg ggtgactttt tgcttgccga
     1141 ggcactcctg cttaatttgt gtgggaaagt tccggacagg agtctgggag gttagcgagg
     1201 atgtgatagc ccgttaatgt gcgctcttgt tctctgatat tgcctttaaa tgtcttggtt
     1261 agcagtattt cgctaatctt taatccagtt aatttaatgc ttcatatggt gaatacacgc
     1321 aggcggtgga gtatctgcta gctctatcaa gagcctgctt ctgtcctgcg gcgttactcg
     1381 aaagctggcg ctccggggtg gtttcagact ttgaccgaac tcgccggtgt cgtcctcggg
     1441 gttttttgcg caagccggat gccgggcgag ttttgctagg gtgttgggta caaatcttcc
     1501 ttctttcaaa ccgagcccaa agcccaagcc aaagccgaca ttcctcagcc acaatacttt
     1561 tccactcgct tggtgcgagt ctgagggtgt cgggttgatt ggtaaggtga tgggtctttg
     1621 tttgaacagg ctgatttgtc ggttgtcttc taatccaccc ttcttgaaag gactgaccat
     1681 gcagctcgtt ctcgaacctc cccgcggcgg caagagctgg attttcttcg ccaacgacct
     1741 ggcccacgac gaaggcttcg tggcctacga gccctgctac agccgcatcc ccgactccga
     1801 gcgctggact gtgaaccagt acctgtgcga ggcggcggag gagttcggca tcaagcccca
     1861 catcttcaac cagtgccagt gggaaggctc caccggctgg gagttctgga ccgatgaccg
     1921 gcccaagatc aaggctgtgg tggagaaggt ggcggaacgc ctcggtctga cggtggatac
     1981 ttccgccctg ggctgacgct ttcgcctctg tctataacct gaaatcgaga gaggaccgat
     2041 gctctgtcgc tcgaattgtg gggtttgctg cacggcaatc tccatttcct cgcccatccc
     2101 cggcatg
//
LOCUS       DAOQNZ010000749         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34283_length_2106_cov_3.348123, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000749 DAOQNZ010000000
VERSION     DAOQNZ010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34283_length_2106_cov_3.348123"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="PKW73_12900"
     CDS             <1..1733
                     /locus_tag="PKW73_12900"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNA74235.1"
                     /translation="AVCGRLGLLATSGSEVSKLYGLKVRTRTAAINAGILPKMIESAD
                     MTEESVRASGIQAPIMIMRSDGGVMDIAAMRKRPILSILSGPAAGVAAAMMFLRISDG
                     VFLEVGGTSTDISAIASGRALVRSAEIGGHKVYMRTLDVRTIGVAGGSLVRLAGGNII
                     DVGPRSAHIAGLKYAAFPTDAVKKSLNGESSSAASCFTVKCIRPLPKDSEDYLALSLD
                     SASGGNGSSQQLHTLTPTCASNLLGLVPEEDCARGDLESIKAGFAALAAYLKKDGVAP
                     AVQAELARRILTLAAAKCEPTVRALIQENKLDPELTLLVGGGGGAAAIVPFLAATMKL
                     SHNLATHADVISAIGVAMALIRESVERQVADAASASEAILSLRADVFNSVHAMGADPA
                     TIEVHVEVDSKTNIIRATACGAAKASTDSVSRTLSDDQCKEKAAQSMRLKPEEVREVC
                     RSSVFTVYGGTVHRQGSIFALKLPLLTSSSFALRVVDKEGIVRLSSRAGAAEKSSKEG
                     TEDLIRRMVEEHSTWGDAGKTIPDILLLLGARVIDLSGLLNEEQVLSLARSELANLPA
                     GSEVIVVAKF"
     gene            1766..>2106
                     /locus_tag="PKW73_12905"
     CDS             1766..>2106
                     /locus_tag="PKW73_12905"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNA74236.1"
                     /translation="MLKGLEEFNGGHYFACHETLETLWKGLSGDEKTLVQGIIQVSVA
                     YYHLNRGNTKGALRLFDRALPRIEPYLPTWLDLDLAAFFQAIRVERERLAAQGPDLPF
                     IASPPLLLRLKA"
BASE COUNT          413 a          567 c          618 g          508 t
ORIGIN      
        1 tggccgtttg tgggcgtctt ggtttgcttg ccacctccgg ttccgaggtg agcaagctct
       61 atggcttgaa ggtgcgaacc cgcacggcgg ccatcaatgc cggtatctta cccaagatga
      121 tcgaatcagc tgatatgacg gaagaaagcg tgcgtgcttc cggaattcaa gctcccatta
      181 tgatcatgcg ctccgatggc ggtgtcatgg acatcgccgc catgcgcaag cgtccgattt
      241 tgtcgattct gtccggacct gcggccggtg tggctgccgc catgatgttt ctgagaattt
      301 ccgatggtgt tttcctggag gtgggaggta cctccacgga tatttccgcc atcgccagcg
      361 gcagagccct ggtacgctcc gcggaaatag gcggtcataa agtttatatg cgcactctgg
      421 atgtgcgcac aataggcgtg gccggtggtt ctctcgtgcg tcttgccggc ggcaatatta
      481 tcgatgtcgg tccgcgcagc gcccacattg ccgggctcaa gtatgccgct tttcccaccg
      541 atgccgtcaa gaaaagtctg aacggggagt cgtcttctgc ggcatcctgc tttaccgtca
      601 aatgtattcg ccccctgccc aaggacagcg aagactatct ggccctcagt cttgatagcg
      661 ctagcggggg caatggctcc agccagcagt tgcatacgct tacccccacc tgtgcttcca
      721 atttacttgg gcttgtgccg gaagaggact gcgccagggg tgatctggag tctatcaagg
      781 cgggttttgc cgctcttgcc gcttatctca aaaaggacgg tgtagctcct gccgttcaag
      841 ctgaactggc ccggcgtatt ttgaccctgg cggccgccaa gtgcgagccg acggtcaggg
      901 cccttatcca ggagaacaag cttgatccgg aactgacttt actggtgggc ggcggcggcg
      961 gtgccgctgc cattgtgccc tttctggctg cgaccatgaa gctgagccac aatcttgcca
     1021 cccatgccga cgtcatttcg gccatcggcg tggccatggc tttgattcgg gagtcggtag
     1081 aaagacaggt cgccgatgcc gcatccgctt ctgaggctat cctatctctg cgtgccgatg
     1141 tcttcaattc ggtgcatgct atgggggccg atccggctac catcgaggtg catgtggaag
     1201 tggacagcaa gaccaatatc attcgtgcca ccgcttgcgg ggcggctaag gcttcaaccg
     1261 acagtgtcag ccgcaccctg agcgacgatc agtgtaagga gaaagcggca cagtccatga
     1321 ggcttaagcc cgaagaagtg agggaagtat gccgctcctc cgtcttcacc gtctatggtg
     1381 gcactgtgca caggcagggc agtatctttg ctctcaaatt gccgcttttg acaagcagtt
     1441 cctttgctct tagagtagtg gacaaagagg gcatagtgcg gctttcgtcc cgggccggtg
     1501 ccgctgaaaa gagcagcaag gaaggtaccg aggacctgat ccggagaatg gtggaagaac
     1561 attcaacctg gggcgatgcc ggtaaaacca tcccggacat actcttgctt ttgggcgcca
     1621 gagtaatcga tctctccggg cttttaaacg aagaacaagt gctttcgctg gcccgttcgg
     1681 aacttgctaa tctgcctgcc ggttctgagg ttattgtggt ggccaagttt tgatttcttg
     1741 cttgaatgaa gagcaagagg tgctattgct taaaggcctg gaagaattca atggcgggca
     1801 ttattttgct tgccatgaaa ccctggagac tctctggaag gggctctccg gggacgagaa
     1861 gactcttgtg cagggcatca ttcaggtgag cgtggcttac tatcatttga accgcggtaa
     1921 tacaaaaggt gccctgcgcc tctttgatag agcgctgccc cgcattgagc catatttgcc
     1981 gacctggctt gatcttgacc tggcggcctt ctttcaagct atcagggttg aaagagagcg
     2041 tttggcggca caggggcccg acctgccttt tattgcttcg cctcctcttt tgctccgcct
     2101 gaaggc
//
LOCUS       DAOQNZ010000750         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34316_length_2105_cov_3.282927, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000750 DAOQNZ010000000
VERSION     DAOQNZ010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34316_length_2105_cov_3.282927"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..1745)
                     /gene="lpdA"
                     /locus_tag="PKW73_12910"
     CDS             complement(276..1745)
                     /gene="lpdA"
                     /locus_tag="PKW73_12910"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNA74237.1"
                     /translation="MVVGEMVQEVDVAVIGGGPGGYTAALRAAELGLRTILVDAQPKP
                     GGVCLHMGCIPSKSLLHAAEVLDSAKQAGKIGITFGEPKIDIKTLRDWKQSVLDKLAG
                     GIVGMCKSAGVEILNGKAIFQDSRSVRVDCPGESAVRIKFKHCILATGSRPTQLPKLF
                     ANPEDMNSERVMDSTSALALKDIPKKLVVIGGGYIGLELGTVYASLGSEVTVIEMTDG
                     LLPGADRDLVKPLAAKLTELFKGIHLSTKVTGIKVTDKGVEISVEGKDLPEKISADKV
                     LISVGRRPNSDGIGLENTEVEVDQFGFVPSDESCRTRDKRILSIGDISGQPMLAHRAI
                     RQGYVAAEVLAGKPSRFDNRVIPAVVFTEPEVAWCGLTEGEAKAKGLEFQVARFPWSA
                     SGRAMTLGGAAGSTKILFATDSQEILGVGIVGPRAGDLISEACLAIEMGAVLEDLIVT
                     IHPHPTLSETLNEASLSAMARLDRARAKEIEKQITSTQG"
     gene            complement(1790..>2105)
                     /locus_tag="PKW73_12915"
     CDS             complement(1790..>2105)
                     /locus_tag="PKW73_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNA74238.1"
                     /translation="EIAEKTRTGKIELERLSGGTFTLTNIGAIGGTSMSPMINFPEAA
                     ILGMARASQKPIVKDGQIAVGTILPLALSFDHRLADGAEAAYFVRHVVERLEDPIKFL
                     LG"
BASE COUNT          399 a          576 c          614 g          516 t
ORIGIN      
        1 cccgcttgat ttgcttgaga actgtcctct ggcatcgtta cctctcaacg ggaaagtatt
       61 ctccataatg gtagcctcca tggcatcctt catgggcccg tttgagtact tttgatagag
      121 attccggaaa caaaatatca gtcggactga gcgtttctga atttacagcc ggaagtctcg
      181 tgcagtctca aagcaaacct gaaaggccat tgcttctagc gcagcggcga taccaccatt
      241 taggctatgc ccgcaaaaaa atcgccctaa gcaatttaac cctgggtaga tgtaatttgt
      301 ttctctattt ctttagcccg ggcccggtcc aacctggcca tggctgacag gctcgcctcg
      361 ttcagggttt ctgagagggt ggggtgaggg tgaattgtaa caatcaggtc ttccagcacg
      421 gcacccattt cgatggccag gcaggcctcg gaaatgagat ccccggcccg gggtcctacg
      481 atgcctaccc ccagaatttc ctggctgtcc gtggcgaaga gaatcttggt ggagccggcg
      541 gcgccgccca gggtcatggc tctgccggaa gccgaccagg ggaagcgagc tacttgaaac
      601 tccagtcctt tggctttcgc ttccccttcg gtgagaccgc accaggctac ttccggttcc
      661 gtgaagacca cggccggtat gacccggttg tcgaagcggg agggtttgcc ggccagcact
      721 tcggcggcca cgtagccctg ccggatggcg cggtgggcca gcatgggctg gccggagatg
      781 tcgccgatgg agaggatgcg tttgtctctg gtgcggcagc tttcatcgga gggcacgaag
      841 ccgaactggt ccacttccac ttccgtgttt tcaagaccga tgccgtcgga attgggccgc
      901 cggccaactg agatgagtac tttatcggcg ctgatttttt cgggtaggtc tttgccttcc
      961 acggaaattt ccacgccttt gtccgtgact ttgatgcctg ttactttggt gctcaggtgg
     1021 atgcctttga agagttcggt cagcttggcg gccagaggtt tgaccagatc ccggtcggcg
     1081 cccggcagaa gtccgtcggt catttctatt accgtcactt cggagccgag ggaagcatag
     1141 accgtgccca gttccagacc gatgtagccg ccgcctatca ccaccagttt tttgggaatg
     1201 tctttgagag ccagggcgga ggtggaatcc atgacccgtt ccgagttcat gtcctcggga
     1261 ttggcaaaga gtttgggtag ttgagtgggg cgcgaaccgg tggccaggat gcaatgtttg
     1321 aacttgatgc gcactgcgct ttcgccgggg cagtccactc ttaccgagcg ggaatcttga
     1381 aagatggctt tgccgttcag gatttccact ccggcacttt tgcacatgcc gacgatgccg
     1441 ccggccagct tgtcgagaac gctctgtttc cagtctctca gggttttgat atcaattttg
     1501 ggttcgccga aggtgatacc aattttgccg gcttgtttgg ctgaatcaag aacttctgcg
     1561 gcgtggagca gtgacttgga gggtatgcag cccatgtgca ggcagacacc gcctggtttc
     1621 ggctgggcat caacgaggat ggtgcgcagc cccaactcgg ctgctctcag ggccgccgtg
     1681 tagccgccgg gaccgccgcc tatgacggct acgtccactt cctgaaccat ttctccaaca
     1741 accatggtcg actcctcaat ttaaatggtg atgcgttctt gggcgctctt tagccgagca
     1801 gaaacttgat gggatcttcc agtctctcca ccacgtgtct gacaaagtag gctgcttccg
     1861 ctccgtcagc cagtctgtga tcgaaggaaa gagccagggg caagatggtg cccacggcaa
     1921 tttgcccgtc cttcacgatt ggtttctgag aggctctggc catgccgaga atggctgctt
     1981 ccgggaagtt gatcataggt gacatactgg tgccgccgat ggcaccgata ttcgtcaggg
     2041 tgaaagtgcc gccggagagc ctctccagct cgattttgcc ggttctggtt ttttcggcta
     2101 tctcc
//
LOCUS       DAOQNZ010000751         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34318_length_2105_cov_3.194146, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000751 DAOQNZ010000000
VERSION     DAOQNZ010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34318_length_2105_cov_3.194146"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1543
                     /locus_tag="PKW73_12920"
     CDS             284..1543
                     /locus_tag="PKW73_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74239.1"
                     /translation="MTSFNSSKSRLIAGALVLSQTFFLAPLAVLANANVQIAGVPVIT
                     SKVSVGGLSPDARAEVIQKNLDNALVASKDRSPASVNITYVKGSPVITLGGYKIVEVD
                     AGNAKAYGTTPALLAKKWADALRNSLVDQASVESYVAQLTGEYSNSAPAVAQAPPVRQ
                     QSYSQTAPAPSDFYATQGNAPTQQTYNMPPQGYPQQYGQAQYGAPPQGGFRQGRVTYA
                     PAGLTIPINLQTAISTQVAKPGDLIQATINQSVMLGDSQIPAGSLVIGTVTDASAGKY
                     LGRASSLTIKFNRLRTPDGIETPITAHLVGGIGKYTDGGSDMDDTLRGETWKTKVGQG
                     AIRGAIGAGTGAALGTAVGAIAGGRGGVGRGAWSGTAIGAGAGVAQSLLLRKGKEVTI
                     PSGTAMQLQLDAPVSIAGGGMPMSGNF"
     gene            complement(1731..>2105)
                     /locus_tag="PKW73_12925"
     CDS             complement(1731..>2105)
                     /locus_tag="PKW73_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74240.1"
                     /translation="VRVGLNFAMAPAGVFSFGAMPVALGAIGAVNPNFAFGKPVGLNV
                     RHRRLKGFAWGFLSGIPGSWLIEDTTVKGQEAIIKPGDEFYAAFKQEFNGEPETDASI
                     MAGASTKVRGQVVNEKPDSKKK"
BASE COUNT          533 a          589 c          552 g          431 t
ORIGIN      
        1 taatccccca gaaaacgccg aaaaatgcgc tcatacgggg caacgaatca agagcccgga
       61 actgacaggg gacgggcttt tcaaaagttt ccaacaggtc ttagcccgga ctcagaaagt
      121 acctcgggaa cattggtaaa ctttttggtg tcatagagcc cattggagag cacaggaagc
      181 tgcgtggcac ctgaaggcac tcaacatagg tcaggtatga cagttacgct gaagacgacc
      241 tgcaactgga ctcgaactaa ctctggcaag gtgaaaaaat aagatgactt cgttcaactc
      301 ttctaaaagc agacttatcg ccggtgctct ggtcctgagc caaacattct tcctggcacc
      361 acttgcggtg ctggcgaatg ccaacgttca gatcgctggt gtccccgtta ttacaagcaa
      421 ggtgtcagtc ggcggtttga gcccggacgc ccgtgctgaa gtaattcaaa agaacttgga
      481 caatgcgctc gttgcatcga aagatcgcag cccggcgtcc gtcaatatta cttacgtaaa
      541 aggcagcccc gtcatcactc tgggcggcta caaaatcgtt gaagtcgatg ccggcaatgc
      601 aaaggcatac ggaacgacac cggcgcttct ggctaagaag tgggctgatg cgctgcgtaa
      661 ctctctggta gatcaagcca gtgtagaaag ctatgttgca caattgaccg gagaatacag
      721 caatagcgct cctgccgttg cacaggctcc gcccgtcaga cagcaatctt attcgcaaac
      781 cgctccggcg ccttccgatt tctacgccac acaaggcaac gctccgactc aacaaaccta
      841 caatatgcct ccgcaaggct acccacagca atacggacaa gcgcaatatg gcgctccgcc
      901 acaaggtggt ttccgtcagg gtcgcgtcac ctatgcgcct gccggcttga ctataccgat
      961 caatcttcag actgcgatct ccactcaagt tgcaaaacct ggtgacctga ttcaagcaac
     1021 catcaatcag agtgtcatgt tgggtgattc tcaaatcccc gccggctctc ttgtaattgg
     1081 aacggttaca gatgcttccg ctggtaaata cctcggtcgc gcatccagct tgaccatcaa
     1141 attcaatcgc ctgcgcactc ccgatggaat cgaaacaccc atcacggctc acctggtcgg
     1201 cggcatcggc aaatacaccg atggcggcag cgacatggat gacacactga gaggtgaaac
     1261 ctggaagacc aaggttggtc aaggtgcaat ccgtggcgcc atcggcgccg gcaccggagc
     1321 cgcacttggc accgcagttg gtgctattgc cggcggcaga ggtggagtag gcagaggtgc
     1381 ttggtctggt accgcaattg gtgcaggcgc cggtgtcgca cagagcttgc tcctgagaaa
     1441 gggcaaagaa gtgacgattc cttccggtac cgctatgcag ttgcaactcg atgctccggt
     1501 atcgatcgca ggcggcggca tgccgatgtc cggcaacttc taattgccgg taaggacgaa
     1561 aagctaacag gaagccgaaa ggagctgcat cagcagctcc tttctcatta ggggcttcag
     1621 gaaatagctg cacgccaggc tacggagtct gcaacaaaag gctaaagcaa aagcgccggg
     1681 ctaacccggc gcttctgaat acaacaaatg gaacaaccag gtgtagaagg ctatttcttc
     1741 ttcgagtctg gcttctcgtt tacgacctga ccacgcacct tggtagaagc accagccata
     1801 atcgaagcat cggtttcagg ctcgccgttg aattcttgct tgaacgcggc atagaattcg
     1861 tcgcccggtt tgatgattgc ttcctgtcct tttacagtcg tgtcttcaat caaccagctg
     1921 cccgggatac cgctcaggaa gccccaagca aagcctttga gacgacggtg acggacgttc
     1981 aatccgaccg gcttgccgaa tgcaaagttg ggattcactg cgccgatagc gccaagagcg
     2041 accggcatag ccccgaagct gaaaacacca gccggagcca tcgcgaagtt gaggcccact
     2101 cttac
//
LOCUS       DAOQNZ010000752         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34365_length_2104_cov_2.628599, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000752 DAOQNZ010000000
VERSION     DAOQNZ010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34365_length_2104_cov_2.628599"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKW73_12930"
     CDS             <1..306
                     /locus_tag="PKW73_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74241.1"
                     /translation="PWKMDQEAHLIKVLDRSRVRVLVSSPSLSLEDPDKEGVLACIFP
                     FGKGYVLHMVGHFDNNARIAVGNFLPDSATDIGISLRQALAANFVAAALKGERPEPR"
     gene            377..1351
                     /locus_tag="PKW73_12935"
     CDS             377..1351
                     /locus_tag="PKW73_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNA74242.1"
                     /translation="MSDSMSKSVNSESDCSPSRIKALEFFSGIGAFSQAVLKRKAEPA
                     LQVLKAFDQNEHANRVFSANYGHTPCSVNLDSISAAQINKGLPLPADLWWMSPPCTPY
                     SRRGKRKDMEDPRAVSFLHLLDLLPQFLPTFLFIENVEGLENTRMEEHLLECLQKSRY
                     CFEKVALCPSQFGVPMLRPRLFYMAWLQPSCIKLPPLQAVPECERKLSAYLEPAEPVV
                     LLGQEELARYGNVLNIVDPEQPEARAICFTSGYFRCRKAAGSLLGGREPVRFFSPREI
                     LNLLGFCKNFILPADLSLNAAYRMVGNSVDVRSIEYLLSGLMPEGAPK"
     gene            1348..1959
                     /locus_tag="PKW73_12940"
     CDS             1348..1959
                     /locus_tag="PKW73_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNA74243.1"
                     /translation="MKPSFRPARGRSYIAFYKPFEVLCQFSEEKESGEEKKTTLAAFD
                     FPASVYPVGRLDYDSEGLLLLSDDGRMTRELFEGREHPRTYLVQVERVPEAAQLLQLE
                     KGVLLQGVKTRPAKVKLLTDEPQIEERCKPIRFRKSVPTAWLELTLWEGKNRQVRRMT
                     AHVGHPTLRLLRVSIGQLRLSDLGLKCGQWRSLSPEEVLKIFA"
BASE COUNT          450 a          552 c          600 g          502 t
ORIGIN      
        1 ccctggaaaa tggaccagga agcgcatctg atcaaggttc tggatcgctc ccgggtgagg
       61 gtgctggtat catcgccctc tctcagtctg gaagacccgg acaaagaggg tgtactggcc
      121 tgcatcttcc cctttggcaa gggctatgtt ttgcatatgg tggggcattt cgacaacaat
      181 gctcgtattg cagtaggtaa cttcctgcct gatagcgcca ccgacattgg catttctctg
      241 cggcaggccc tggcggccaa ttttgtcgcc gccgccctga agggggaacg ccccgagcca
      301 aggtgagcga ttttgcagga agatcgccgc acaggagcgc tttagagttg gtaagcttgc
      361 cttactgaag gtacctatgt ccgattccat gtctaaaagc gtcaactcag aatctgattg
      421 ctccccgagc cggatcaagg ccctggaatt tttttccggc ataggggctt tttcacaggc
      481 cgttctgaag cgaaaggcgg agcctgccct gcaagtcttg aaagcctttg atcaaaatga
      541 gcatgccaac cgggtattct cagcaaacta cggtcatact ccttgctcgg tcaacctgga
      601 tagcatttcc gccgcacaga tcaataaggg gctgcccctg ccggccgact tgtggtggat
      661 gtcacctcct tgcacgccct attcaagaag gggcaagcgg aaagacatgg aggacccgag
      721 agccgtttcc ttcctgcacc tgcttgacct tttgccgcag tttctgccga cctttctctt
      781 catcgaaaat gtagaggggc tggaaaacac aagaatggaa gaacaccttt tagagtgcct
      841 gcaaaaatct cgctactgct ttgaaaaggt agctctctgc cccagtcagt ttggtgtacc
      901 gatgctccgt ccccgtctct tctatatggc ctggcttcag ccttcgtgta tcaagttgcc
      961 gccccttcag gcagtgccgg agtgtgagag gaagttgagc gcctatctgg aaccagccga
     1021 gccggtggtt cttctcgggc aggaggaact ggcgcgttat ggtaatgtgc ttaacattgt
     1081 cgatcctgag cagcctgagg cccgcgccat ttgttttacg agcggctatt ttcgttgccg
     1141 caaagcggcc ggctctttgc tgggcgggcg agaaccggtg cgctttttta gccccagaga
     1201 gattctcaac ttgcttggct tttgcaagaa tttcatcctg ccggcggatc tgtctttgaa
     1261 tgcggcctat cgtatggtcg gcaatagcgt cgatgtgcgt tcaattgaat atttgctcag
     1321 tggcttgatg ccggaggggg ctcctaaatg aagccatctt tccgaccggc ccggggtaga
     1381 agctatatcg ccttctacaa accgtttgaa gttctctgcc agttctctga ggaaaaggaa
     1441 agcggcgagg aaaagaaaac aaccctggcc gcctttgatt tcccggcctc ggtctatccc
     1501 gtcggtcgcc tggattacga ctcggaaggc ttgttgcttt tgagcgatga tgggcgcatg
     1561 accagagaac ttttcgaagg ccgtgagcat ccccgcactt atctggtgca agtggagcgc
     1621 gtgcccgagg cggcgcaact tttgcagttg gagaagggcg tgctcttgca gggtgtgaag
     1681 accagaccgg ccaaggtgaa gctgcttacg gatgagccgc agatcgaaga gcgctgcaaa
     1741 ccaattcgct tcaggaagag tgtgcctacg gcctggctag agttgactct ctgggaggga
     1801 aagaatcggc aggtgcggcg aatgacggct catgtgggac atcccacctt gcgcttgttg
     1861 cgcgtgtcca tagggcagtt gagactttcc gatctgggtc tcaaatgcgg acagtggcgc
     1921 agtctcagcc cggaagaggt tttgaaaatt ttcgcctaag gtataaaaca ccttagtatg
     1981 aaagagcgca agcaaaaacc ggcagaacat accgaaggaa aagccgtcgc tgcttctcct
     2041 gcctccgccg ttgagacttt ccgatctggg tctcaaatgc ggacagtggc gcagtctcag
     2101 cccg
//
LOCUS       DAOQNZ010000753         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34425_length_2102_cov_4.598437, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000753 DAOQNZ010000000
VERSION     DAOQNZ010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34425_length_2102_cov_4.598437"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2102)
                     /locus_tag="PKW73_12945"
     CDS             complement(<1..>2102)
                     /locus_tag="PKW73_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74244.1"
                     /translation="LTNLLSYNAVVLLFYWFIRQITAAWSNTRSGAAALFASAIFLAS
                     PLHCESVSWVVGRVDIVSALFYLLSLNLFLLAKAVFPIKSRYRLYLTLALTSFVLAMW
                     TKEMPIGVPVLLPFLLFFFGKGDGSLRSVIKQCLPIWLTTVIYLILRYLALGTLLGGY
                     VQGIGDAQAAHALSRWLDKDTLIRLFYPLPFGIFGSNHLYQHCLTLLYAVSTGLLMVR
                     LFCLRAPVKMLLFLPVWIVSTLIPIYKLWGLGYELEGARFCFFLTLPLSAALPLLLLA
                     PVEKGNRTGDQKNFSMAAYFLLAVSTMTVLTAVLTKICAATNLAWVHTGKEVKEFCLQ
                     ARALADRQDEKLLLLGIPKRRGGTHMILNGTTFNILLSPPYTKNKEAQAAGITAGNTT
                     ATKPVRIETFDPIIFGNSEYIDSERFKRLAGEKPTILVWLGELRRFLNVSYAAPLSEN
                     LRPLILPGLNNDSGSSSKDRSGVQEPCTGFLHTMGHAVVRAAKDGLLIDNILEGDGIA
                     FSGLNLNPLSADYLEVKLTVLKPGTGRLKAALQASAGSTSSPEHNPASADDQQRALAA
                     IYAPYEVEAYLPEIDALNQVQAGKKQEREETVLLPLSDNWRWYVGDIDTIVVNLPASA
                     NLKLHSVALKKACQVRPSLRPAHLKCSSTGVYYRENSDLTLTAVFQKDCQALSGMENL
                     VVEITRANTFFENLAPQA"
BASE COUNT          482 a          504 c          639 g          477 t
ORIGIN      
        1 ccgcctgtgg cgctaaattt tcaaagaagg tgttggctct ggttatttca accaccagat
       61 tttccatacc gctcaatgcc tgacaatctt tttgaaagac tgccgtcaag gtcaggtcgc
      121 tattttctcg ataatagaca ccggtggatg agcatttgag atgcgccggt ctgagcgagg
      181 ggcgcacttg gcaggctttc ttcagtgcca ccgaatgtag tttcaggttg gcggaagccg
      241 gtaaattcac tacgatagtg tcgatgtctc ccacatacca gcgccagttg tcgctcaagg
      301 gaagcagtac ggtctcttct ctttcttgct tcttaccggc ttgtacctgg ttaagggcat
      361 cgatttccgg taagtaggct tccacttcgt agggggcgta gattgcggcc agggctcttt
      421 gctggtcgtc ggcgcttgcg ggattatgct cggggctcga ggttgagcct gccgacgcct
      481 gaagcgctgc tttcagtcgc ccggtgccag gttttaagac ggtcaatttg acttccaggt
      541 agtcggcgct gagggggttc aaattgagac cgctgaaggc gatgccgtcg ccttccagaa
      601 tgttgtcgat gagcagaccg tcttttgctg cccggaccac ggcatgcccc atggtgtgca
      661 ggaaaccggt gcagggttct tgcacaccgg agcggtcttt ggaactactg ccgctatcgt
      721 tattcagtcc gggcaggatc agtggtctca aattctcgct gagcggagcg gcgtaactga
      781 cattcaggaa gcgcctcagc tctcccagcc agactagtat ggttggtttc tcgccggcca
      841 gtcttttgaa gcgctcacta tctatgtatt cggaattgcc gaaaatgatc gggtcgaagg
      901 tttctatccg gactggtttt gtggcggtgg tgttaccggc agtgataccg gcggcttgcg
      961 cctctttgtt tttggtatag gggggcgaga gcaagatgtt gaaagtggtg ccgttcaaga
     1021 tcatatgggt tccgccgcgg cgtttgggaa taccgagcag caatagtttc tcatcctgcc
     1081 tgtctgccag agcgcgagcc tgcaggcaga attccttcac ctctttgccg gtatgaaccc
     1141 aggccaggtt agtggcggcg cagatcttcg tcaatacggc agtgaggacc gtcatggtgg
     1201 aaacggccag cagaaaatag gcggccatgg aaaagttctt ttgatcgcct gtacggttgc
     1261 ccttttccac cggagcaagg agtagcaagg gcagggcggc actcaaaggt aaggtcaaga
     1321 agaagcaaaa gcgtgcgcct tccaattcat agccgagacc ccagagtttg tagatgggaa
     1381 tcaaggtgga cactatccat accggtagga aaaggagcat ttttaccggc gcccgcaagc
     1441 agaaaaggcg caccattaga agacctgtgc ttacggcata gagcagggtg aggcagtgtt
     1501 ggtagagatg attggagccg aaaatgccga aaggaagggg gtagaagagt ctgatcaggg
     1561 tgtctttatc aagccagcgg ctcaaggcat gtgccgcctg ggcatcaccg atgccctgca
     1621 cgtatccgcc caggagcgtg cccagagcca gatagcgcag aattagatag ataaccgtgg
     1681 tcagccagat tggcaggcat tgtttgatga cgcttctgag gctgccgtca cctttaccga
     1741 aaaagaagag cagaaatggc agcaacaccg gaaccccgat gggcatttcc ttggtccaca
     1801 tggccagcac gaagctggtc agggccaggg ttagatagag cctgtaccga cttttgattg
     1861 gaaagactgc tttggcaagc aggaagagat tgagggaaag cagatagaag agagcggaga
     1921 cgatgtctac acgtccgact acccaggaaa cgctctcgca gtgcagtggc gaggccagaa
     1981 agatagccga ggcaaagaga gcggccgcac cggatctggt attggaccag gcggctgtga
     2041 tttgccttat gaaccaataa aagaggagca cgacggcgtt gtaagagagc aagttggtca
     2101 gg
//
LOCUS       DAOQNZ010000754         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34435_length_2102_cov_3.404983, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000754 DAOQNZ010000000
VERSION     DAOQNZ010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34435_length_2102_cov_3.404983"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PKW73_12950"
     CDS             complement(<1..902)
                     /locus_tag="PKW73_12950"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74245.1"
                     /translation="MNLGEISIDVENLKGSRAGRSFDLTLDELHLLETACNYPNWHPT
                     PLELAQAVSRPESPCDEVKLSQLLLALRNKLDPACDTIFPQLFSSTQTATFTRQVDPY
                     VGSVLADRYVLTKLIGGGSSSLVYQGRRRHDNKPVAVKLLIINSRSSPEVLARFKREA
                     RLNSALVHKNIVAVEDYGVGERDQPFLVMELLDGATLQEIVEKHGLPNLVEVLGFFRQ
                     VCEGLAYAHSQGIVHRDIKPNNMMVMGSGESGFTVKIVDFGLARLAENDNPNTEVTQA
                     GNVVGSPPYMSPEQLVGDCTERSDV"
     gene            1240..>2102
                     /locus_tag="PKW73_12955"
     CDS             1240..>2102
                     /locus_tag="PKW73_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA74246.1"
                     /translation="MKTFQELGLSELTIATLTSLGFENPTEVQKATIPSAMEGEDLIV
                     SAQTGSGKTAAYALPIIESLKEVEAPKKTLDQDALAAEGKPLRRLPRWQKMKLFKFEP
                     IFTKALVMVPTRELALQVKEQFIRMNANKALHVVALYGGADYMKQANALKRGAHVIIA
                     TPGRLIDFLERGAADLMQARQVVLDEADRLLDLGFSLQINEILKYLPEERQTLVFSAT
                     IDKRVAAVANAYQKNPKTVQINTGRIEPSTIDQRIHYLKEKEKEAKLLELVTGVEGSI
                     LVFTQTKVKATL"
BASE COUNT          541 a          598 c          525 g          438 t
ORIGIN      
        1 acgtcgctac gttcggtgca atcgccgacg agttgctcgg gactcatata aggagggctg
       61 cccaccacat taccggcctg ggttacttcg gtattgggat tatcgttctc cgccagtctg
      121 gccagaccga aatcaacgat cttgacagta aagcctgact caccgctgcc catgaccatc
      181 atattgttgg gcttgatgtc ccggtgcacg atgccctgac tgtgggcgta agccagccct
      241 tcacagacct gccggaagaa gcctagaacc tctaccagat tgggcaaacc gtgcttttcc
      301 acaatctctt gtagggttgc cccgtccagg agttccatga ccagaaacgg ctgatctcgc
      361 tcgcccacgc catagtcttc caccgccaca atatttttgt gcacgagagc actgttcaag
      421 cgtgcttctc gcttgaagcg ggccagcact tcaggggaac tgcgggaatt gataatgagg
      481 agcttgaccg ccacgggctt attgtcatgg cgccggcgcc cctggtaaac caaactggaa
      541 ctgccaccgc cgattagttt ggtcagaaca tagcgatcag ccagcaccga gcccacataa
      601 gggtcaacct gcctggtaaa agtagctgtt tgggtgctgg agaaaagctg cggaaaaatg
      661 gtgtcgcaag ccggatcgag cttgtttctc agggctagca aaagctgaga aagcttcacc
      721 tcatcacagg gactctccgg tcttgacaca gcctgagcca gttctagcgg cgtaggatgc
      781 cagttgggat agttgcaggc agtctcaagc aaatgcaact catcgagcgt caggtcgaag
      841 ctgcgcccgg cacggctgcc cttgagattc tccacatcaa tgctgatttc accaaggttc
      901 atatgtaaga cgggggtcct cagttgtgcg gtcaggattc aactataaag gttaccaccg
      961 ccgtgaaaaa taagcgtgga attagccggg cggcaccggt actttagaac agtggcagca
     1021 ccattttatg agacacatta aagcccgcca atttacctag tttacaaaga cgcccaatcc
     1081 ttttgacatc caagcagcgc gtatgtaata ctccatggac ttctgtcaag agtcttattg
     1141 tgccagcacg ctcgcattaa tgagaacggg cagccaggga cgcaaaaagc gtcccaatac
     1201 cgtcgcaatt aatgcgcaat cacaataaag gactccattt tgaaaacttt tcaagagctc
     1261 ggactctccg agcttacaat tgctactctt acttctctag gatttgaaaa tcccacggaa
     1321 gtgcagaaag caactattcc ctcggctatg gaaggggaag acctgattgt ctcagcccag
     1381 accggctcag gtaaaaccgc tgcctacgcc ctgcccatca tcgaaagcct caaggaagtc
     1441 gaagccccca agaagaccct cgatcaagat gccctggccg ctgaagggaa gcccctgcgg
     1501 cgcctgcccc gctggcagaa gatgaagctc ttcaaattcg aacctatctt cactaaggcc
     1561 ctggtcatgg tgcccactcg cgaactggcc ctgcaagtaa aagagcagtt tatcaggatg
     1621 aatgccaaca aggcattgca tgtggtggca ctctacggcg gtgccgatta catgaaacaa
     1681 gccaacgcac tcaagcgcgg cgcccatgtg atcatcgcta cccccggtcg cctcatcgac
     1741 ttcctcgaac gcggagcggc cgatctcatg caggcccgtc aagtggtgct cgatgaagcc
     1801 gacagacttc tagatctggg cttcagcctg caaatcaatg aaatcctcaa gtatctgccg
     1861 gaagagagac agaccctggt cttctccgcc accatagaca agcgggtggc tgccgtagcc
     1921 aacgcttatc agaagaaccc taaaacagtc cagatcaaca ccggtcgcat cgaacccagc
     1981 accatcgacc agcgtatcca ctatctgaaa gaaaaagaga aggaagcgaa gctcctggaa
     2041 ctggtaaccg gtgtggaagg ttccatcctg gtcttcacgc aaactaaggt aaaagccacc
     2101 ct
//
LOCUS       DAOQNZ010000755         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34491_length_2100_cov_4.160880, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000755 DAOQNZ010000000
VERSION     DAOQNZ010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34491_length_2100_cov_4.160880"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PKW73_12960"
     CDS             complement(<1..668)
                     /locus_tag="PKW73_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNA74247.1"
                     /translation="MVKFKDYYEILGVERKAGADDIKAAYRKMARKYHPDANKNDPKA
                     EERIKEINEAYEVLKDPEKRSRYDQLGANWKSGDNFRPPPDSGFSFDFGNLGDLHNNK
                     SFSDFFDMLFGQTFGSPQQQKAHGGTTRRQVLDQEADVELTLEELARGAKRTLQLSAP
                     GGKPRTIEVKIPAGLRPGKKVRVPGEGATAGGSTVKGDLYLRIKVKPHPYFTIDGDNL
                     VCELP"
     gene            complement(792..1415)
                     /locus_tag="PKW73_12965"
     CDS             complement(792..1415)
                     /locus_tag="PKW73_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74248.1"
                     /translation="MVSLRRILAQGRRRNIGASAQMAEFAPAFMIFILVIMLPFINLL
                     SFATGYASLAFLAQNSAVQASNSSTYAQALQEMSNSANQTKSSGIGQFAKLAPVGGFN
                     GCGCDLFITETDLTTKVSTQYGPNTAVTVTPDPTNKIYEFQVRATYDIGPFLNLGSVP
                     FVSSVPLVGAPVRITISSHRAAEHTDALTFLAGTTKIAVLKEPVVSY"
     gene            1753..>2100
                     /locus_tag="PKW73_12970"
     CDS             1753..>2100
                     /locus_tag="PKW73_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74249.1"
                     /translation="MAGLIILVPIVLVLFDLAVIVLGVQINDSTCREAARVASIGDPT
                     DCDKRARAVVARANAQGSSMLSNFQMFSCTSTVTPAQVNAMKLNFGGPVSGTVTVVTR
                     VDIHPFVVHYAYSG"
BASE COUNT          410 a          516 c          608 g          566 t
ORIGIN      
        1 ggcaattcgc agacgagatt gtcaccgtcg atggtgaagt aaggatgtgg tttcacctta
       61 atgcgcaggt agagatcgcc cttgactgtg ctaccgccgg cagtggcacc ttcgccgggt
      121 acccgtactt ttttccctgg tctcagtccg gccgggatct tgacttcgat ggtgcgaggt
      181 ttgcctcccg gtgcgctcag ttgcaaggtg cgtttggcgc ctctggccag ttcttccagg
      241 gtcaattcca cgtccgcttc ctgatcgagc acctgtcttc tggtcgtacc gccatgggct
      301 ttctgttgtt ggggtgagcc gaaggtctga ccaaagagca tgtcgaagaa atcggagaac
      361 gacttattgt tgtgcaggtc gcccagattt ccgaagtcga atgagaagcc gctgtcgggc
      421 ggtggtctga agttgtcgcc actcttccag ttggcgccaa gttggtcata gcggctgcgc
      481 ttttccgggt ctttgagaac ctcgtaggct tcgtttattt ccttgatgcg ctcttctgct
      541 tttggatcgt ttttgttggc atccggatgg tatttcctgg ccatcttgcg ataggctgcc
      601 ttgatatcat cggcgccggc tttacgttcc acacccagta tttcgtagta gtccttgaac
      661 ttgaccattc agacctgtct ttcagtacgg aagctaagtg taattctata actaatagca
      721 tgaataggcc ggcaagcagc aatcaagaga ctcgattcaa gcaagagtgc atggattgag
      781 accggtgcgg ctcagtagga aactacaggc tctttgagga ccgctatctt ggttgtgccg
      841 gcaaggaaag tcaaggcgtc ggtgtgttct gcggcccggt gggaggagat ggttattcgc
      901 accggtgcgc ccaccagggg caccgaactg acgaagggga ctgagcccag attgaggaag
      961 ggaccaatgt cgtaagtggc tctcacctga aactcgtaga tcttattggt agggtcgggg
     1021 gtgacggtga cggcggtgtt gggaccgtat tgggtggaaa ctttggtggt caggtctgtt
     1081 tcagtgatga agaggtcgca gccgcagccg ttgaaaccgc ctaccggtgc taacttggca
     1141 aattgtccga tgcccgatga tttcgtctgg ttggctgaat tgctcatctc ttgcagcgcc
     1201 tgagcgtagg ttgaagagtt ggaagcttgc acggcgctgt tttgcgctag aaaggccagt
     1261 gaggcgtagc cggtggcgaa gctcaagagg ttgatgaagg gaagcatgat gaccaggatg
     1321 aaaatcatga aagccggtgc aaattcagcc atttgagccg atgcgccgat gttccttcgt
     1381 ctgccctgtg caagtattct tcttaaactg accatttccc atcccccagc gcctgttcaa
     1441 gttctaccac ggtggagcct aaactatgta tatagtccca aaattcgctg tccgaccctt
     1501 atttgctggt gccggggtgc aggtgagtca gcttctcgga taaatatttt gcgaattgtt
     1561 gtgagaattg cccaaaggtg gaataatcga attagctttg gttaatcttg tgccgggcat
     1621 gcgctcgtca gtttggggcg tctctcgcta gtgcttctac tctgctttca ggttttgctt
     1681 atgccgggtc aggtaatgtt ttcatactca tcaaatcgct tcaggccgcg gggacagtca
     1741 tcagttgaac tggtggcggg tcttatcatc ctcgttccca ttgttttagt actcttcgac
     1801 ctggctgtga ttgtgcttgg tgtgcaaatc aacgactcca cctgtcgaga ggcggcccgt
     1861 gtcgcttcca tcggtgatcc caccgattgc gacaagcgtg cccgggcggt ggtagcaaga
     1921 gccaatgccc agggtagctc gatgctttct aatttccaga tgttcagttg caccagcacc
     1981 gttacgcccg cccaggttaa tgccatgaag ctcaatttcg gcggtccggt aagcggtacg
     2041 gtgaccgtcg tcacccgggt ggacatacat ccctttgtgg tgcactatgc ctactccggt
//
LOCUS       DAOQNZ010000756         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34684_length_2094_cov_4.445316, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000756 DAOQNZ010000000
VERSION     DAOQNZ010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34684_length_2094_cov_4.445316"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..389)
                     /locus_tag="PKW73_12975"
     CDS             complement(21..389)
                     /locus_tag="PKW73_12975"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNA74250.1"
                     /translation="MTNMDGSMPGHDPDCLFCRIGKGQINATYVHEDENCFAIRDISP
                     QAPTHILVIPRLHRQNITMVEDKVLLGDLFQTASRIAADEKLENGFRLVVNTGDHGGQ
                     SVGHLHIHILGGRPLAWPPG"
     gene            555..>2094
                     /locus_tag="PKW73_12980"
     CDS             555..>2094
                     /locus_tag="PKW73_12980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNA74251.1"
                     /translation="MTSESNILPRLLQLQKGLFASLKLKEVLDSAVALFTELAGGAKV
                     AVFLCDNESTCFKLMAAKGYTDGSLDQMKVIPFAIDSMLKSVHQRRVPIACAEASQAP
                     DFSATIMRREASKGQIALPLVSGNLLVGAVLLEVNNPQLLSFLDFLKEVSDLVATAIS
                     NSILFGRSEYERERLNTLYKTSCALNSNALEVGPVLKIAADTALVLGNTPTCAILLLD
                     EGGDKFQLAAFKGLDGPSLSEFDMSFGKSIAGSCLRAGHTEVYGDGQREPFGMPRAMS
                     GRPFASVLAIPLISGERKIGVLEVFSTESRAFHREHQDLLESLAAQVSAALNIALSHE
                     SASLGGSILDAHTGLVNRVHFEAALVKELERSQRHSHNLSVLLVDIDHLSQINEMLGQ
                     MKGDDAIKHVAGTIKSSLRDIDVPSRFGGEEFAVILPETAQEHALEVADRLRQKLRQA
                     PAPGIGLITVSVGIASFPGNADNLANLMRDAEQALNVAKYEGRDRVKTALTGSYKDSG
                     ALSGI"
BASE COUNT          453 a          565 c          578 g          498 t
ORIGIN      
        1 cccagcgggc ggggggtctc ttagcccggc ggccacgcca ggggacggcc gcccagtata
       61 tgtatatgca aatgacccac cgactgccca ccatgatcgc ctgtgttaac taccagtctg
      121 aagccgttct cgagcttttc gtcggcggcg atgcgagaag ccgtctgaaa tagatcgcca
      181 agcaaaactt tgtcttctac catagttatg ttttgtctgt gcagacgtgg aataacaagg
      241 atgtgtgtag gcgcttgtgg gctgatatcg cgaatggcaa agcagttttc gtcttcatga
      301 acataggtgg cattgatttg gcccttacct atgcggcaga agaggcagtc aggatcatgc
      361 cccggcattg agccatccat attagtcatt gaggattctc ctttggcagg gaagccgata
      421 gggtaaaaat tgaaggtgca gtaaatgata aggcaaggcg gctaaaagct tactctaaag
      481 aatttaggac gaaggcgctc ctaaagacga caaacacgtt tatccttaag ttctctgcct
      541 ggagggagag tgcgatgaca agcgaatcaa acatactgcc ccgactgctg caattgcaga
      601 agggactttt tgcttcttta aagttgaagg aagtgcttga ttcagctgtg gccctcttca
      661 cggaactggc gggaggcgcc aaggtggcgg tttttctctg cgacaacgag agcacctgtt
      721 tcaaactgat ggctgccaaa gggtacacgg acggttccct tgatcaaatg aaggttattc
      781 ccttcgctat cgacagcatg ctcaagtcgg tacaccagag acgggtgccg atagcctgtg
      841 ccgaagcctc tcaggctccc gacttttcag ccacaatcat gcggagagag gcctctaaag
      901 gtcagatcgc cttacccctt gtcagcggaa acttgcttgt gggtgcggta ttgctcgagg
      961 tcaacaatcc ccagcttttg agctttcttg atttcctgaa agaggtgtcg gatctggtgg
     1021 ccacagccat ctccaactcc attttgtttg gtcgctccga gtatgagcgg gagcgcctca
     1081 acaccctcta caaaacctct tgcgccctca attccaatgc cctggaagtc ggtccggttc
     1141 taaagattgc cgccgatacg gctctggttt taggaaatac ccctacttgc gccattctcc
     1201 ttcttgatga aggcggcgat aagttccagt tggcggcctt caaggggctc gacggcccca
     1261 gcctgagcga gtttgacatg tcgttcggta aatccattgc cggcagttgt ttgcgggccg
     1321 gtcacacgga agtctacgga gatggtcaac gggagccctt tggcatgccc agggccatga
     1381 gcggtagacc ttttgcttcg gttctggcca taccgcttat cagcggcgag cgcaagattg
     1441 gggtattgga agtcttttcc acggaatcgc gggccttcca ccgtgagcat caagatcttc
     1501 tggagtctct ggccgcacag gtctctgctg ctttgaacat cgccctttct cacgagtcgg
     1561 ccagcctcgg cggttccatc ctcgatgccc acaccgggct ggtcaatcgg gtgcacttcg
     1621 aagcggctct ggtgaaagaa ctggagcgct cccagagaca ttcccataat ctctcggtct
     1681 tactggtgga tatcgaccat ctttcccaga tcaatgaaat gctcggccag atgaagggtg
     1741 acgacgccat caagcatgtg gccggaacca tcaaaagttc gctgcgcgat attgatgtgc
     1801 cctcccgctt cggcggcgaa gaattcgctg ttattctgcc tgagaccgct caggagcatg
     1861 ctctcgaggt ggccgatcgc ttgcggcaga agctgcggca ggctcccgct cccggcatcg
     1921 gcttgataac tgtttccgtg ggtattgcct ccttcccggg taatgccgac aaccttgcca
     1981 acctgatgcg tgatgccgag caggccttga atgtggccaa gtatgaaggg cgcgaccggg
     2041 tcaagaccgc tctcaccggc agctataaag acagcggcgc cctctccggt atta
//
LOCUS       DAOQNZ010000757         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34792_length_2091_cov_3.445972, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000757 DAOQNZ010000000
VERSION     DAOQNZ010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34792_length_2091_cov_3.445972"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PKW73_12985"
     CDS             complement(<1..945)
                     /locus_tag="PKW73_12985"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74252.1"
                     /translation="MEPDSQGANRQEQEHTQRVDGMTSAESVIARGPTSVSGYRPGQI
                     IGGHYEVIELVGKGGMSLVYKTRHLLLNQLRAIKIILPQMDKDGRGLQRFQQEAKLAT
                     LLEHKNIVRTMEFASPSDGPPFLVMDFVEGRSLEQELSVLQRLSAERAVKIVLQIADA
                     LEYILSQGIIHRDLKPGNIILVKEKSHPGKGMPQDLVKLIDFGIARTITPDGDDSQGI
                     LYTPTGEVVGSPQHMSPEQCAGETLDERSEIYSLGCILYELLAGKPPIVGKNSLDTLR
                     LQASVEARPLNKVVPDTAHIKELSRITSTCLEKDPEHRY"
     gene            1049..1408
                     /locus_tag="PKW73_12990"
     CDS             1049..1408
                     /locus_tag="PKW73_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74253.1"
                     /translation="MLSFYVYQFLPIVIVEAIVLWRMHWGSLPRCFYDSLMMNFASFL
                     GLVLGLGPYLTGSSPWGLTLFGTYSVMVEGTVLMLLERRDARQVWACALTANLLGCIL
                     LAIEVLVRSIPELLSVK"
     gene            1537..>2091
                     /locus_tag="PKW73_12995"
     CDS             1537..>2091
                     /locus_tag="PKW73_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA74254.1"
                     /translation="MSQTITQEVIWQPSGKYLESRVAQFMKEHQISDWRQLIERSNED
                     TEWFWENALKYMGFQWHKPYSKLKDESKGFAWTKWFVDGQLNIISNILDFHMEEGKVA
                     GCRRSVGKDHPAMIWEGEDGRHRKLTYGELNSLSGKIAQAMLNLNVKAGDAVGIYMPM
                     VPEVVAVLFACLKIGAVAVPVFSGY"
BASE COUNT          433 a          529 c          590 g          539 t
ORIGIN      
        1 atagcgatgc tcgggatcct tctccaggca ggtggaagtg atgcgggaga gttccttgat
       61 atgagcggtg tcaggtacca ctttattgag gggacgggct tctacgctgg cttgcaggcg
      121 taatgtatcg agggaatttt tgcctacgat gggcggctta ccggccagaa gctcataaag
      181 gatgcaaccc agtgagtaga tttccgagcg ctcatcgagg gtttcaccgg cgcattgctc
      241 cgggctcatg tgctgcggtg agcccactac ttcaccggtg ggagtataga gaatgccctg
      301 actgtcgtca ccgtccgggg tgatggtgcg ggctatgcca aagtcgatta gcttgaccag
      361 atcttgaggc atgcccttgc ctggatgtga tttctctttt accaggatga tattgcctgg
      421 tttcagatct cggtgaatga tgccctggga gagaatatat tccagggcat cggcgatttg
      481 caaaacaatt ttgaccgccc tttcggcgct caggcgctgc agcaccgaga gttcctgctc
      541 cagagagcgg ccttccacaa agtccataac caggaagggc ggaccgtccg atggcgaggc
      601 aaattccata gttcgcacta tgttcttgtg ctccagcagg gttgccagtt tggcttcctg
      661 ttgaaagcgc tggagccccc gcccgtcctt atccatctgg ggcagaatga tttttattgc
      721 tcgcaattgg ttgagaagca gatgcctggt cttgtagacc agactcatgc cgcctttacc
      781 aacgagttct ataacttcgt agtgaccgcc gattatttga ccgggcctgt agccgctcac
      841 gctagtggga cccctggcga tgacgctttc ggctgaggtc atgccgtcca cccgctgggt
      901 gtgctcctgc tcttgcctgt ttgccccttg agaatccggc tccatttcaa tcaagttcct
      961 tagagagcag aaactacatt ctactagtaa gatggagtga ccggtgccct gtcccgccct
     1021 gaaaattatt ccctgactga gcctgcctgt gctgagtttt tatgtctatc agtttctgcc
     1081 tattgtcatc gtggaagcga tagtgctctg gcgtatgcac tggggcagtc tgcctcgctg
     1141 tttttatgat tccctgatga tgaattttgc ctcgtttctg ggtctggtgc tggggctcgg
     1201 accttatctg accggcagta gcccctgggg actgaccctc tttggcactt attcggtcat
     1261 ggtggaaggc accgtgctca tgcttctgga aagacgtgat gctcgccagg tatgggcttg
     1321 cgccctgaca gccaatttgc tgggctgcat tctactggcc atcgaagtgc ttgtgcgctc
     1381 gattcccgaa ctgctctcag tcaaatagga aatttatctg agtttcaggc ttgtaaagag
     1441 ccacttcccg gtaatatgag cgtcgttcgg tgctccgcgc cctgtcttgg gtgccgcaag
     1501 caccttgtac ccctcttcta aaagagtgct tttgcaatga gtcaaaccat cacccaggaa
     1561 gttatttggc agcccagcgg caagtatctg gaaagtcgcg tcgctcagtt catgaaagag
     1621 catcagattt ccgactggcg gcagttgatc gagcgctcca atgaagacac cgagtggttc
     1681 tgggaaaatg ccctcaagta catgggcttt cagtggcaca agccgtacag caaattgaag
     1741 gatgaaagca agggtttcgc ctggaccaaa tggttcgtgg acggccagtt gaatatcatc
     1801 tccaacatcc tcgacttcca catggaagag ggcaaggtgg ccggttgccg cagaagcgtg
     1861 ggcaaagacc atccggctat gatctgggaa ggtgaagacg gtcggcatag gaaacttacc
     1921 tatggcgaat tgaattcatt gtcaggcaaa atagcccagg ctatgctcaa tcttaatgtg
     1981 aaagccggag acgccgtcgg catctatatg cccatggtac cggaggtggt ggcagtgctg
     2041 tttgcctgcc tgaagatcgg tgccgtggct gtgccggtat tcagcggtta t
//
LOCUS       DAOQNZ010000758         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34879_length_2088_cov_4.389080, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000758 DAOQNZ010000000
VERSION     DAOQNZ010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34879_length_2088_cov_4.389080"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKW73_13000"
     CDS             <1..346
                     /locus_tag="PKW73_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74255.1"
                     /translation="MFEGNAYTRIRDGSTLCLMTERQESAEIVAKLLLLTGNGWEVLY
                     RVSEGINNPGFYYDPAHAQKRLAEVKEHGELVKRLMQYAALLYGDRLYKADGTEVAEA
                     DYNLVGNRKQSL"
     gene            474..959
                     /locus_tag="PKW73_13005"
     CDS             474..959
                     /locus_tag="PKW73_13005"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNA74256.1"
                     /translation="MNNLKTGVLLVASSVLDRDPDFRQSLLFLAAYDGAFVFALNIAG
                     PVEEDWLTIGGPLDSPLFMVSKVNDATKEYATGDSGYATVPIMQDGKEPRDFKDIVAP
                     LIAKNKDHCLAITGHAGWGQGQLEWEVETGYWQVLDVSLDEFLRLPPEARWQHARTML
                     A"
     gene            1016..1330
                     /locus_tag="PKW73_13010"
     CDS             1016..1330
                     /locus_tag="PKW73_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74257.1"
                     /translation="MKNNTIVTIASFAIATAANNQLAIALTVELATGETLEIVLTSES
                     ATDLHNRLLGLLKVAGVSSSDELVDSEILVEVENGEIVALKHLLEARHLVLGTLKLAK
                     AA"
     gene            1356..1670
                     /locus_tag="PKW73_13015"
     CDS             1356..1670
                     /locus_tag="PKW73_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74258.1"
                     /translation="MENDLLIAQATLRDMQATLSAMETKLWLVYAAVNLMFLFTGIFM
                     SFRQEAKKLHLIFGAGYGAASLPAVYTNISSLPMFLLPALCYLAGVWSGGRLKAKLKT
                     QS"
     gene            1749..>2088
                     /locus_tag="PKW73_13020"
     CDS             1749..>2088
                     /locus_tag="PKW73_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74259.1"
                     /translation="MSHGSSPLILATLASAVVSLCLMIHLLKQGFRKECLKSACETAL
                     LFTAIAVLTASAGGGLHDFLIELAFYVTGILMMHETSAEEGQLGLWGNLRYSSVTDGI
                     GDTWRSRLPVI"
BASE COUNT          544 a          551 c          533 g          460 t
ORIGIN      
        1 catgtttgaa ggaaatgcat acacccgcat aagggatggc tccaccctct gtcttatgac
       61 tgaacggcag gagagcgccg agatcgtggc taagctattg ctcctcaccg gtaacggctg
      121 ggaagtactc taccgagtca gcgaaggcat taacaatccg ggtttctact acgatccggc
      181 tcacgctcaa aagaggcttg ccgaagtgaa ggaacacgga gagctggtca aaaggctgat
      241 gcaatatgca gccttgctct atggcgatcg cctgtataaa gcggacggca cggaggtggc
      301 ggaggcagac tacaacctgg taggcaaccg caaacaaagt ctgtaattca ggaccaacag
      361 tcggccggga tttcaggtta gcgaaagaaa caaacgttct acaacaccgc cccaccgaag
      421 ctgtgctcag ctccagtggg gcaccacttt ttatatctga agaggaaaaa caaatgaaca
      481 acttaaagac aggtgtgctc ctggtggcaa gctccgttct ggaccgggat ccggactttc
      541 gccagtcgct actctttctg gcggcatacg atggggcttt cgttttcgct ctcaacattg
      601 ccggaccggt tgaggaagac tggctgacaa tcggtggtcc gctggattca ccgctcttta
      661 tggtcagcaa agtaaacgat gctactaaag agtatgccac gggcgatagc ggctatgcca
      721 ctgttcccat catgcaagac ggcaaagagc cccgcgactt caaggatatc gtggcccccc
      781 tgattgccaa aaataaagac cattgcctgg caataacagg acatgcaggc tggggccagg
      841 ggcagttgga atgggaagtc gaaaccggct actggcaggt gctggacgtc agtctggatg
      901 agttcttaag actacctccc gaagctcgct ggcaacatgc cagaactatg ctcgcttaaa
      961 cgctgcctct caaaccgtca gcaataaatc taaatttcaa accggagtac ttactatgaa
     1021 aaacaacaca attgtaacca ttgcttcttt tgctatcgct actgccgcca acaatcaatt
     1081 ggcaattgca ctgacggttg aattggcaac cggagaaact ctggaaatcg tgctcacatc
     1141 cgaatcggct accgacctgc ataatcgcct gctcggactg ctcaaagtag ccggtgtgag
     1201 tagcagcgat gagctggtgg actcagaaat actcgtggaa gtagaaaacg gcgaaatcgt
     1261 cgcattgaag cacctgcttg aagccagaca tctggttctg ggaactctga aactggcaaa
     1321 agcagcctaa caactgaaat tactcggagc ttactatgga aaacgatctg ctcatcgctc
     1381 aggccactct gcgcgacatg caagcaaccc tttcagccat ggaaactaaa ctctggctgg
     1441 tctatgcagc tgtgaacctg atgttcttgt tcaccggcat ctttatgagt ttcagacagg
     1501 aggcgaagaa gctccacctg atcttcggcg cgggctacgg tgcagcatcc ttgcctgctg
     1561 tatacaccaa cataagcagc ctgccaatgt ttctgttacc ggcgctgtgc tacctggcag
     1621 gagtctggtc gggaggcaga cttaaggcaa aactgaagac tcaatcctga ctttacccaa
     1681 ccaggacagg cgcaggacag ctccaatcgc cagggacggc acaattcaac cattcaccgg
     1741 agatgaacat gtcacacggt tcttcacctt tgattcttgc tactctcgcc agcgctgtgg
     1801 tgtccctatg cctgatgatc cacctactga aacagggatt tcgaaaagag tgcctcaaga
     1861 gcgcctgcga aacggctttg ctattcactg caatcgccgt actcactgct tctgccggag
     1921 gcggactaca tgatttcctg atcgagctgg ccttctatgt caccggcatc ctcatgatgc
     1981 atgagacaag tgccgaagag ggtcaacttg gtctttgggg caacctacgc tatagttctg
     2041 ttaccgatgg aatcggcgac acctggagaa gcagattgcc agtcatct
//
LOCUS       DAOQNZ010000759         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34899_length_2088_cov_2.912445, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000759 DAOQNZ010000000
VERSION     DAOQNZ010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34899_length_2088_cov_2.912445"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2059)
                     /gene="glyS"
                     /locus_tag="PKW73_13025"
     CDS             complement(<1..2059)
                     /gene="glyS"
                     /locus_tag="PKW73_13025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNA74260.1"
                     /translation="MSDYLLEVGVEELPAGFVPEALDRLCQLLKAELEANSIEFSEIA
                     SYATPRRLAALVKNISKMQKTTEKTVKGPPVKSSFDENGKPLGAALGFAQKNGLTVEE
                     LTREEVAGVTYLFARVVTPGKPTKDVLPALIEKIIPQISGERLMRWGSCSVKFSRPLR
                     WFVSLLDKEVVPFSLAGIESGRHSRGHRILHGGQVEISEAISYEGDLEKAFVMVDAGR
                     RQAKIEKEVKDLAGSLKGHAMRLDKNSSLVEEVVYLTEWPVAVMGDLEPEYLALPSML
                     IETIMVHHQRYFPLSKDEKVDEEAVDHKLLPHFITISNNDSAGALSKIKQGNERVLRA
                     RLADGKFFYFDDGKQKLQDRSEALGKLTYQEGLGSYKDKTERMVELAGLLAEQSGTDG
                     GDRKALLRTCALMKLDLVTGLVRELPELQGFVGSWYAGKDGEDKSVVRAIASHYAPRF
                     SQDAIPQDRVGQLAAVVDKLDHVTGLFALGKKPSGSSDPYALRRNAQGLIDILVDGMP
                     AVPVNLVELIDKLMTRFEPLVNGNGKFNRQEKSLEVQDFLLQRLKGKLLDLSYSREIV
                     ECAMATGSLENIADLLVRLTVLKELAEDAGKQGLLRVGVRIGNILKADSKAEVDAQTL
                     TEPEEKALWQAFQVRSEQLKVPELPTANQYKQLCSHLSELVEPVDAFFDKILVNDEDR
                     QKRQ"
BASE COUNT          455 a          592 c          525 g          516 t
ORIGIN      
        1 tctgacgttt ctgccggtct tcatcgttca cgaggatctt gtcgaagaag gcgtccacag
       61 gctctaccag ttcgctcaag tgggagcaca actgcttgta ttgattagcg gtcggcagct
      121 caggtacttt cagttgctcg cttctcacct ggaaagcctg ccagagagct ttctcttccg
      181 gctcggtcaa ggtctgagcg tcaacttcag ctttggaatc ggctttcagg atgttgccga
      241 ttctcactcc cacccgcaag agtccttgct tgccggcatc ttccgctagc tctttcagca
      301 cggtcaggcg caccaataag tcggcaatat tctccaggct gccggtggcc atggcacatt
      361 ccacaatctc tctggaatag gagagatcaa gcagcttacc tttcaatctt tgcaaaagga
      421 aatcctgcac ttccagcgac ttttcttgcc ggttgaactt gccgttgccg ttcaccagag
      481 gttcgaacct ggtcatcaac ttatcgatga gctcgaccag gttgaccggc actgcgggca
      541 tgccgtccac cagaatgtcg atgagcccct gcgcgttgcg ccgcagagca taaggatcgg
      601 aagaaccgct gggcttctta ccgagggcaa agagcccggt gacatgatcg agcttgtcca
      661 caacggccgc cagttgaccg acccggtctt gcggaatggc atcctggctg aatctaggcg
      721 catagtgcga ggcgatggcc cgcaccacgg atttatcttc accgtcctta ccggcatacc
      781 aactgcccac aaacccttgc aattcaggca gttcgcgcac cagaccggta accaggtcaa
      841 gcttcatgag ggcacaggtg cgcaacaaag ctttcctgtc accaccatcg gtgccgctct
      901 gctcagccaa tagaccggcc agttccacca tgcgctcggt cttgtctttg tagctgccca
      961 ggccttcctg gtatgtcaac ttgcccaggg cctcactgcg atcctgcaat ttttgtttgc
     1021 cgtcatcaaa gtagaagaac ttaccgtcgg ccaggcgggc tctcaaaact cgctcattac
     1081 cttgcttgat cttggagagg gcgccagcgc tgtcattgtt ggaaattgtg atgaagtgcg
     1141 gcaaaagctt gtgatcgaca gcctcttcgt ccactttctc atctttagaa agggggaagt
     1201 accgctggtg gtgcaccata attgtttcga tgagcatgct cggcaaggcc agatactccg
     1261 gctccaaatc gcccatgact gcaacgggcc attccgtcaa gtaaaccact tcctccacca
     1321 gagaagagtt tttgtcgagc ctcatggcat gaccttttag actaccggcc aagtctttta
     1381 cttctttctc tatcttggct tgtcggcggc cggcgtcaac catgacaaaa gccttttcga
     1441 ggtcgccttc ataagatatg gcttcggaaa tttccacttg cccgccatgc aaaatgcgat
     1501 gaccgcgaga atgcctgccg ctctcgatgc cggccaggct gaaaggtaca acctctttgt
     1561 caaggagcga tacaaaccat cgcagcgggc gagaaaattt gacggaacag gatccccagc
     1621 gcatcaagcg ctcgcctgaa atctggggaa tgattttctc aatcagagcc ggcagcacat
     1681 cttttgtagg tttgccggga gttactaccc gggcgaacaa gtaggtgact ccggcaactt
     1741 cttctctggt caattcctcc acggtcagac cgttcttctg ggcgaagccc agggccgcac
     1801 cgagaggttt gccgttttcg tcaaaactgg atttaacagg gggaccctta actgtctttt
     1861 cggtagtttt ctgcattttg cttatatttt ttacaagcgc agccagacgt ctgggggtgg
     1921 cgtacgaggc gatttcggag aattctatgg aatttgcttc cagctcggct ttgaggagct
     1981 gacacaatct atcaagagcc tcgggcacaa agcctgccgg caattcctct actcccactt
     2041 ccaaaagata gtcagacatg gtggtccttg caaatgtgtg atgaaact
//
LOCUS       DAOQNZ010000760         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34920_length_2087_cov_4.207185, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000760 DAOQNZ010000000
VERSION     DAOQNZ010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34920_length_2087_cov_4.207185"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKW73_13030"
     CDS             <1..556
                     /locus_tag="PKW73_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74261.1"
                     /translation="RAWLSRMKRSMLSLCPVFNINRMVREYATEMYFPSLVRYHDFVE
                     NGASKAKALAHWKENLKQKWSHVRIGAVEENTGDTIKVGDDMNISAWINLGELNAEDV
                     VVQMYHGPIDQYGNIVEGEVIDMRPDSSAKKGSEKLFTGAIKYFKSGRHGFTVRVLPS
                     HEDLVSSFDTPYICWATEPKQVKV"
     gene            810..>2087
                     /locus_tag="PKW73_13035"
     CDS             810..>2087
                     /locus_tag="PKW73_13035"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA74262.1"
                     /translation="MVDNKTQRLLSSEQIKTRLEREMKSLEGKLSVLFEAAARSKGKS
                     APGSTAADAEADFAVCGSRLIEIYDRLGFTARAEEFLARLESSYFKDSQWFLHTYYQS
                     RDHRKLVEMLWRSYHLRERELPQEQRCCDLTRIADLHYKLGQMDKAESTYKLASDMAL
                     QRALEMAPGDDGKRYVSIGSIYSYACFLAVRGRLEPATALYERCLGFWEREKEFNFGS
                     GYAGFDYLGTFLELIKDKDVQLHKRLQSRWQSVFRDRKFAGILDKKGVEVTAPEFAPF
                     QDLVKVSNFSQGLGAAAHKHSRLFGYVDTRGNWKIDPRFIRANAFSSGVATVKLSSGL
                     FPIDVEGQWTPYSLIDKNGKEVLRLPDMAVGDFEDGLARGYRHAHSRFGLSDLFHENG
                     DILFSGTFIGPLKRDGNRFSLFIITGESKRGCIV"
BASE COUNT          485 a          480 c          588 g          534 t
ORIGIN      
        1 gcgtgcctgg ttgagccgta tgaagcgctc catgctatcg ctttgcccgg tcttcaacat
       61 caacagaatg gtgcgtgaat acgccactga gatgtacttc ccgtccctgg tgcgctatca
      121 cgattttgtg gaaaatggtg cctccaaagc caaggctctt gctcactgga aagagaacct
      181 gaagcagaag tggtctcatg tgcgcatcgg tgcggttgaa gagaataccg gtgacaccat
      241 caaggtaggc gacgatatga atatcagcgc ctggatcaac cttggtgagc taaatgccga
      301 agacgtggtg gtgcagatgt atcacggacc catcgatcaa tatggcaata ttgtggaagg
      361 ggaagtgatt gatatgcgtc cggattcttc tgccaagaag ggttccgaga agctgttcac
      421 cggcgccatc aagtacttca agagcggcag acacggcttt accgtaaggg tattgccgag
      481 ccacgaagac ctcgtttcga gcttcgatac gccatatatt tgctgggcca ctgagcccaa
      541 acaggtgaag gtgtaaggct tttgctcaag gtgtttgatc gccggcaaga caatacaaga
      601 atgagatgga cccggatttt tccgggtcct ctccattaga agagacccgc agtatgaaga
      661 gaccgccatc ctggaagaga ttgtctggtc gaaagttttt ccttgccaca gttttgtgcg
      721 gcatggttct attcttgccc ttcttctttt ctgagcaggc gtctcaagct cagccttggc
      781 cggatgacta caaccttcag aataattgga tggtggataa caagactcag cggctattaa
      841 gttctgagca gataaagacc aggctggagc gggaaatgaa gtcactggaa ggaaagcttt
      901 cggtgctgtt cgaggccgcc gcccgttcaa agggaaagtc ggcgccgggc tcaaccgccg
      961 ctgatgccga ggcagatttt gctgtttgcg gctcacgctt gattgagatc tatgatcggc
     1021 tgggtttcac ggccagagct gaagagtttc tcgccagact tgaaagtagt tactttaagg
     1081 atagtcagtg gtttttgcat acttattatc aaagccgcga tcaccgcaag cttgtggaga
     1141 tgctatggcg aagctatcac ctgcgggagc gggaacttcc ccaggagcag agatgctgtg
     1201 acctgacacg cattgccgat ttgcattaca aacttggtca aatggataag gctgagtcta
     1261 cctataagtt agcctccgat atggctctcc agcgtgccct tgagatggcc ccgggcgatg
     1321 acggtaagcg ttacgtcagt atcggttcga tatatagcta tgcctgcttt ctggctgtgc
     1381 gagggcggct tgagccggcg acagccttgt atgagcgctg tctcggcttt tgggagcggg
     1441 aaaaggagtt caattttgga tctggatatg ctggcttcga ctatcttgga acctttctcg
     1501 aactcatcaa agataaggat gtgcagcttc ataagagatt gcagagccgt tggcagtcag
     1561 tttttcgtga ccgaaaattt gccggcatct tagataagaa aggtgtggag gtgacggctc
     1621 ctgaatttgc gcccttccag gaccttgtca aagtttctaa tttctctcag gggctcgggg
     1681 cggcagccca taaacacagc cggctctttg gttacgtgga tacccgcgga aactggaaga
     1741 tagaccctcg ttttattcgc gccaatgctt tcagtagcgg ggtggccaca gtcaaacttt
     1801 ctagcgggct tttccctata gatgtagaag ggcagtggac accgtattcg cttatagata
     1861 agaacggcaa ggaagtcttg cggttgcctg atatggccgt cggtgatttt gaggacgggc
     1921 ttgcccgggg ctatcggcat gcccacagtc gttttggact cagtgatctg tttcatgaaa
     1981 acggcgatat cctgttttct ggtaccttca ttggcccttt gaaacgagac ggaaaccggt
     2041 tctcgctttt cattattacc ggtgaaagca agaggggttg tatagtc
//
LOCUS       DAOQNZ010000761         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34946_length_2087_cov_2.429134, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000761 DAOQNZ010000000
VERSION     DAOQNZ010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34946_length_2087_cov_2.429134"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="PKW73_13040"
     CDS             <1..1520
                     /locus_tag="PKW73_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA74263.1"
                     /translation="AEQKTLRNMLMPLERVRSEATELDGLFTSLDDSFQKGMRALDTQ
                     DQVDATVAYIQARRTTVKVENLARRLRLKLNEQRQIFAERQAQTSTRTYILVGAGFVF
                     NILLALLLFLYFQRRTSRRFALLTENITALGLERPLLNALAGVDELAQLDRTLHKVSE
                     SLKEARIKEQAILTNAADFICSIDAGLRLTAINPAVEAVFAVSQDELLGRNINSLVPA
                     KKAQFVLERFKQIAQDRAGSFELAIVNHDGREVELSWNVIWSEPLQSYFCVAHDITER
                     KKLERLKQEFVAMLSHDMRAPLTSLQLTLNVLSSTADQLNEKSAQRVARAEASVKVLV
                     DMISELLEMEKLQSDVFVLECQTCSLNNLVEEALSLVEESGKAKHLSLKKALEPIEVS
                     CDGERIVRVLTNLLFNAIKFSPQGGQIAVTISRSGEFVTVEVSDQGPGIPENMQELVF
                     ERFRQVDSGEGRGASASGTGLGLAICKAIIAAHRGEIGVRSQLGKGSVFWFKIPV"
     gene            1597..>2087
                     /locus_tag="PKW73_13045"
     CDS             1597..>2087
                     /locus_tag="PKW73_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74264.1"
                     /translation="MKRKTLQVVFTLTFISMGLALVSYLIPVPPVLAGTAGLNTVRRY
                     ADDDDRFSLNYFMNTHPDLQKNAEAMELWGLSMLSNKRNQEALSTLSQANALAKGNFN
                     TAASLAYGLCLTGKGDQGMSLIDKVLAGDADNCRALTVKSLLLGLSGKEVGADQCLKH
                     AESV"
BASE COUNT          477 a          503 c          588 g          519 t
ORIGIN      
        1 gggccgagca gaaaactctg cgcaacatgc tcatgccttt agagagagtt cgcagcgagg
       61 ctaccgaact ggacggactt tttacctcgc tggatgattc cttccagaag gggatgcggg
      121 ctctcgatac tcaggaccag gtagatgcta ccgtggctta tattcaggct cgtcgcacta
      181 ctgtcaaggt cgagaatctg gcccggcgcc tgcgccttaa attgaatgag caaagacaga
      241 tctttgccga aaggcaggcg cagacaagta ctcgcaccta tatactggtg ggcgccggtt
      301 ttgtattcaa catattactg gcgcttctac tctttctcta ttttcagcgc cgcacatcca
      361 ggcgctttgc tctacttacg gaaaatatca cggcccttgg tctggagcga ccgcttctaa
      421 atgcccttgc cggtgtggac gaactggcac aactggaccg caccctgcat aaagtgtcgg
      481 agtctctgaa agaagcccgc atcaaagagc aagcgatttt gaccaatgct gccgatttta
      541 tttgctcaat tgatgccggt ttgcgtctga cggccatcaa tccggccgtg gaagccgtct
      601 ttgcagtcag tcaggatgaa ttgcttgggc gcaacatcaa ttccctggtg cccgctaaga
      661 aagcgcagtt cgtgctggaa cgcttcaagc agattgccca ggatagagcc ggctcgtttg
      721 agctggccat cgtcaaccac gacggaaggg aagtagagtt atcctggaat gtgatctggt
      781 ccgagccgct tcagagctac ttctgtgttg ctcacgatat caccgagcgc aaaaaattgg
      841 agcgtttgaa gcaggagttt gtagccatgc tctctcacga tatgcgtgcg ccgcttactt
      901 cactgcaact gactttgaat gtactctcct ccactgctga tcaattaaat gaaaaatcgg
      961 ctcagcgtgt ggcccgtgcc gaagcctcgg tgaaggtgct ggtagatatg atcagcgaac
     1021 tgttggagat ggagaagcta cagtcggatg tgttcgttct ggaatgccag acctgctctt
     1081 tgaataatct ggtggaggaa gcgctttcac tggtggagga aagcggtaaa gctaagcatc
     1141 ttagcctcaa aaaggctctg gagcctatcg aggtcagttg tgacggggag cgtatcgtgc
     1201 gagtcttgac caatttgctc ttcaatgcca tcaagttttc accacaggga gggcaaatcg
     1261 ccgtcaccat aagcagaagc ggtgaattcg tgacagtgga agtgtccgat cagggaccgg
     1321 gtataccgga gaatatgcaa gagctggtgt tcgagcggtt tcgccaggta gactcgggag
     1381 aggggcgggg ggcgtcagcc tcggggaccg gtcttggtct ggccatctgt aaggctataa
     1441 tcgccgctca caggggcgag ataggggtgc gcagccagtt ggggaaaggc agtgtattct
     1501 ggtttaaaat acctgtttag attcgaagtt tttataggaa gccaggagtt ttggtcggtg
     1561 ttcttgtcag gcagtactaa aaacaggtac tctcgtttga agcgtaagac gctgcaagtc
     1621 gtcttcactc tgacttttat ttccatgggc ttggctctgg tatcgtattt aataccggtg
     1681 ccgccggtgc tggccggtac cgccggtctc aacactgtca ggcgctatgc cgacgatgac
     1741 gatcgcttca gcttgaatta ttttatgaat acccatcctg atttgcagaa gaatgccgaa
     1801 gctatggagc tatgggggct ttccatgctc tccaataaac gtaatcagga ggctctgagc
     1861 acgctatcgc aggctaatgc cctggccaag ggtaatttca ataccgccgc ttccctggcc
     1921 tatggtcttt gtttgacagg caagggcgac cagggcatgt ctttgataga caaggtgctg
     1981 gccggcgatg ctgataattg tcgcgctctt acggtcaaat ctctcttact cggtctcagc
     2041 ggcaaagaag taggcgctga tcagtgtttg aaacatgccg agagcgt
//
LOCUS       DAOQNZ010000762         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34959_length_2086_cov_5.077794, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000762 DAOQNZ010000000
VERSION     DAOQNZ010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34959_length_2086_cov_5.077794"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PKW73_13050"
     CDS             complement(<1..822)
                     /locus_tag="PKW73_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74265.1"
                     /translation="MVEKRRELTTLTSNSQRCLMSDPKLTRTDPSHALAEAQKCLASD
                     NLPEAMLWVGTANVLEQGEERLSLASPVNSACFRLVARALNLAETRIVYCGNILAEHG
                     RMIRPALEALAIITAKLPKHGATSQEFENLTMVAAHLQRLEKTYRLHLDEASFGRYVG
                     TSARQASDFTNTIVACRQLLAKVVEKAIPAGSSKGPDADLVKQVQLSETARMRLAETA
                     ISNAQHHIFGFPIAFLSDFEQAEFFATAAERLTAPAPEAGGDLAAPYRAPVLADFK"
     gene            1586..>2086
                     /locus_tag="PKW73_13055"
     CDS             1586..>2086
                     /locus_tag="PKW73_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNA74266.1"
                     /translation="MLKRLLSSFKALAFVLALGCLGLMAQGGVFTQELLTKFEAQNLQ
                     GVLDKLIPLGVNALVAVIFLGISYLFYKPARAALVRTLDTTGASSRGKTLILRSLQTG
                     YWVFTVFVVASVVAPEVLSKLFLGVSILSAAIALSLQGVASDLVCGVFLQMTRRFQPD
                     DNISIIG"
BASE COUNT          384 a          543 c          652 g          507 t
ORIGIN      
        1 tttgaagtcg gccaggacgg gggcgcggta aggggctgcc aggtcgccgc cggcttccgg
       61 tgcgggagcc gtcaggcgct cggcggcagt ggcgaagaat tcggcctgct cgaagtccga
      121 gaggaaggcg atggggaagc cgaagatgtg gtgctgggca ttggagatgg cggtctcggc
      181 caggcgcatg cgcgcagtct ccgacaactg cacctgcttc acgaggtcgg catcaggacc
      241 cttgctgctt cctgccggga ttgccttttc gacgaccttg gccaggagct ggcgacaggc
      301 cacgatggtg ttggtgaaat ccgatgcctg cctggcgctg gtgcccacgt agcggccgaa
      361 ggaagcttcg tccaggtgca gccggtaggt cttttccagg cgctgcagat gtgcggcgac
      421 catggtcaga ttttcgaatt cctggctcgt ggcgccgtgt ttgggcaact tggcggtgat
      481 gatggccagg gcttccaggg cggggcggat catccgacca tgctcagcca ggatgttgcc
      541 gcagtagacg atgcgagtct cagccaggtt gagagcccgg gccaccagac ggaagcaggc
      601 gctgttgacg ggcgaagcga ggctcaggcg ctcttcgcct tgttcgagaa cattggccgt
      661 gccgacccag agcattgctt ccggcagatt gtcggaggcg aggcacttct gggcttcggc
      721 gagggcatgg gagggatcgg tacgggtgag tttgggatcg ctcataagac acctttgaga
      781 gttggaggtg agagtagtaa gttcacgtct tttttcgacc atttcttctt ccagccggct
      841 tgatagcacc tggtcgtgcc cgttgggcat agcgatgctt cgctccaccg gcggcttgct
      901 taagcaccac aggtggtgta gggcgaagct aggttttcgc tgggggtgtt gggcggttag
      961 aagaagtttt tagttgaaag aaaggtaagt gcatccagaa acctagtaag ttcacgtgcc
     1021 taaatccacg ggcaaggagt catttctggg cttatttctc ttaaaaaatc gctcattccg
     1081 gtggttggag atggtggatg tcaaggtgtg ctagctgtgt ggtgcgggag gctgttgcgc
     1141 caatttccag cttagcgtca ggtgcgctaa ggcggtggga cgtttattag atactcttat
     1201 ttcaaaaaag ctcatgagct aattagagct gtccaatagt tgccaaggaa tatggcttat
     1261 gggcaggctc ttcggtagtt tcaagatgtt cttcttttcc cttccttcaa ttcggtatct
     1321 acctctcttc aatagtcatt gcatcagctc tctaaccaac ttcttgaacc cttaccggtt
     1381 caggattttg tacatggctg attgacggac ggaagcgtgg tgttgctgcg agccctggag
     1441 cctgtttgtt ccctggctat gaatttgaaa tgggttggca gaagagctga aaacgtgccc
     1501 gtgagcacct ctcacgagga atcgcccgcc cggcggttct agatggacca gcaattggtc
     1561 ccaacatctt ctacacggag ttaccatgtt gaaaagattg ctatcgagct tcaaggctct
     1621 agcgttcgtg cttgccctcg gctgcctcgg attgatggcc cagggcggcg tgttcacgca
     1681 agagctgctc accaagtttg aagcccagaa tctccagggc gtcctggaca agctcattcc
     1741 cctcggcgtc aacgcgctgg tggctgtgat cttcctgggc atctcctatc tgttctacaa
     1801 gcccgcccgc gctgccctgg tgagaaccct ggacacgacc ggcgccagct ctcgtggtaa
     1861 gaccctgatc ctgcgttcgt tgcagaccgg ttactgggtc ttcaccgtgt tcgtggtggc
     1921 cagtgtggtg gcgccggaag tgctgtccaa gctcttcctc ggtgtcagca ttctcagtgc
     1981 cgccatcgcg ctctctctgc aaggcgtagc cagcgacctg gtttgcggcg tcttcttgca
     2041 gatgaccagg cgctttcagc ccgatgacaa catttccatc atcggt
//
LOCUS       DAOQNZ010000763         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34967_length_2086_cov_4.058099, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000763 DAOQNZ010000000
VERSION     DAOQNZ010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34967_length_2086_cov_4.058099"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKW73_13060"
     CDS             <1..452
                     /locus_tag="PKW73_13060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA74267.1"
                     /translation="FTHQANLAYVHALDEVTPLARERQLTLDYKTVTGLPLISGNKEA
                     IMGILRQVLERMIVITAAGGRVRIESQLRGNEMRIGVSSSGPALPEFEIQDMFLGFIE
                     GKHALETYGSRLSMQLARTNVERMGGRIWAESEAGRGTVTYFTLPTN"
     gene            complement(454..1572)
                     /locus_tag="PKW73_13065"
     CDS             complement(454..1572)
                     /locus_tag="PKW73_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74268.1"
                     /translation="MNFGISPIQPGDRIILDGGTGGVTYTTALDIGVSGTATNPITIM
                     QSPEPGHNGRVTLSGFVGGAQVRLPVGVRLAGSYIRMYGSRRGGLRISDYLFQGVAVT
                     GSNNWLSNLEIAGVFGPIINPSKVPGVGLLFAGNNNVYKCLDIFNCLLMAQQGFPTSE
                     SNSNFERCWFFDTYNRSAGGFVVNGGREDSRVEVSNCVFGPGLAQGLINKSSSGFLAV
                     NGSLFLNASQDNILLSGAAGTSRTVLRRNTAFMTDANYLGLPHNNLNYNSNGNLTVVN
                     SIFYGGLIRADAAARAKASGNFQYNVLGNTVLLASTLADPQFRQAELIKALKGTTPLK
                     EQAAADFTLADCSPASGKGARATSVKVLNKAFASYFFP"
BASE COUNT          536 a          572 c          528 g          450 t
ORIGIN      
        1 cgttcaccca ccaggccaac ctcgcctacg tgcacgccct cgacgaagtc acccctctgg
       61 ccagggaaag acagcttacc ctcgactaca agaccgtcac cggtctgccc ctgatcagcg
      121 gcaacaagga agcgatcatg ggcatcttac gccaggtgct ggagcgtatg atcgtcatca
      181 ccgccgctgg tggcagggtg agaatcgaaa gccagttgcg cggcaatgaa atgcgcatcg
      241 gcgtatccag ttccggaccg gccctgcccg aatttgaaat ccaggatatg ttcctgggct
      301 tcatcgaagg caaacacgcg ctagagactt acggttcccg cctttccatg cagttggccc
      361 gcaccaatgt ggagcgcatg ggcggcagaa tctgggccga gtcggaagcc ggtcgcggca
      421 ccgttaccta ctttaccttg cctacaaact aagctatgga aagaagtagg aagcaaaagc
      481 cttattcaag accttaaccg aggtggccct tgccccttta ccactggccg gagaacaatc
      541 agccagggta aaatctgcag cagcctgctc tttcaagggc gtagtacctt taagggcctt
      601 gatcagttcc gcctggcgaa actgaggatc ggcaagagtg gaagctagaa gcacggtatt
      661 acccagcaca ttgtactgaa agttgccgct ggcctttgct ctggccgcag cgtcggcacg
      721 aatcaggccg ccatagaaaa tggaattcac cacagtcaaa ttgccgttac tgttgtagtt
      781 aagattgttg tggggcagtc cgagatagtt ggcatcggtc ataaaggccg tgttccgtct
      841 caagaccgtg cgactggtgc cggcagcccc agaaagcaaa atattgtcct gggatgcatt
      901 gagaaagagc gagccgttca ctgccaaaaa tccagaactg cttttattga ttagcccctg
      961 agccagacct ggaccaaaga cgcaattaga tacctcgacc cgactgtctt cacggccgcc
     1021 attgaccaca aaaccaccgg ccgagcggtt gtaggtatcg aagaaccagc agcgctcgaa
     1081 attactattg ctttctgaag tgggaaagcc ctgctgggcc atgagcaagc aattgaatat
     1141 atccagacac ttatagacgt tattgttgcc ggcgaaaagc agacccacac cgggcacctt
     1201 actgggattg ataatcggcc cgaagactcc ggctatttcc agattagaaa gccagttgtt
     1261 ggagccggtg acggctacac cctgaaacaa atagtcggat atgcgcagac cgccacgacg
     1321 actgccatac atccttatat aacttcctgc aagtctcaca cccacaggca agcgcacctg
     1381 cgcaccaccc acaaagccgc tcaaggtgac ccgaccgtta tgaccgggct ccggactttg
     1441 catgattgta atcgggttgg tcgctgtacc acttacacct atatccagag cagttgtata
     1501 agtgacgccg ccggtgccgc catccaatat aattcgatcg cccggctgaa tagggctgat
     1561 accaaaattc aagggcggaa tattgcgtaa ggccgtagac caactcagac cggttgtacc
     1621 gtcaccagcc ggagaaatat agtaatcacg cgcctgacag gcagaacatg ctaagaaaat
     1681 agatgagaga aggagtgccg caactgccag ttccttttta gctgttctag ctgttttcca
     1741 cgggcgaagt aaaccgagcc gccgagcgcc gaacgctcgc ggccttactg aattttgcat
     1801 aatgccgata ctccctgaaa ggcttgaaag ataccagtac agcgtatgga ttagtcgtga
     1861 agtgttcgtg acgcgaaagt gaatttggcg tgatacgcga aatactttat tctcagtttt
     1921 catttaatgt gcgccgccat aatttagaca caagcgcttc ctattatgaa actcacaaaa
     1981 ccttactaat ttttctggag tgcgcatatg actaatcgca ccgcggcttt tgccgcccta
     2041 atctgcctgg catcgctcag tttcaatggg gctgcaatgg cccagg
//
LOCUS       DAOQNZ010000764         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_34995_length_2085_cov_4.649754, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000764 DAOQNZ010000000
VERSION     DAOQNZ010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34995_length_2085_cov_4.649754"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PKW73_13070"
     CDS             complement(<1..1022)
                     /locus_tag="PKW73_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74269.1"
                     /translation="MARTKYKGTRKQPLLLYLVAGCSFMLASFLCVDKEFGLNITYDL
                     SSPFRKQPPIKWRSRTQAISEAKLTRKPLLYAVLYKGDEFAYRLESDGLCQPEIYNYV
                     NENFIPVKVPVSVQPVTDESLGKEFKGFTHQVNMDYQQSGLYVVPYRLLDASGRDIAS
                     TANLIELGATDPENVNPYGDSYGQRFEMGRSSGAFDSLGAHKPPLLVRYRDKEDLLEY
                     LYLAKTWHTLKPTRGLVKWQPSSILNEKTSVRAQVKPRMLFLLADAGYDSDYFRLSSL
                     HSKKLAAIINSKTRPVLVEYHPYEPALDKDIEYLKKQYGVNTLPAVILLDTKDGKPVV
                     QRGNIY"
     gene            complement(1009..1797)
                     /locus_tag="PKW73_13075"
     CDS             complement(1009..1797)
                     /locus_tag="PKW73_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74270.1"
                     /translation="MNRAICVNTWRQFKSNNVRLILLTLLILAPLAHFTWRRCTYEYD
                     LMEDMISYPFVASLFALIWGVGVISCERQHGTISLVLARPLTVSRYVLSKWFAVGFAA
                     SLCSLQCLFVEHAVSVILHPPLLSNLDFVANGLERVILAFGTASMLIFYSSLVTGLKD
                     LALIVGTLFAGMVGMQFLRPILISEVCMRGAGTAGQQFMTHLTNVVGSGFAVFFFPNI
                     PVSEILNGSSAIFTSTINFVCVVTVCLSLAIFFLSRKEFSYGQD"
     gene            complement(1794..>2085)
                     /locus_tag="PKW73_13080"
     CDS             complement(1794..>2085)
                     /locus_tag="PKW73_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74271.1"
                     /translation="RDRESSLSHTLIIKWLLDGAPVEASAFTRVTAHCGATLEQFSPE
                     DGLARVFVDNEEDSASLIKGLVEAGVPVTRVLPELSRLQRVFIDEMRRGEKQ"
BASE COUNT          581 a          515 c          528 g          461 t
ORIGIN      
        1 tcgtaaatat tccccctctg tacaaccggc ttgccgtctt tcgtatcgag caaaatcaca
       61 gccggcaagg tgttaacgcc gtattgtttt ttcagatact caatatcttt gtcgagggca
      121 ggctcgtagg gatgatactc aaccagcacc gggcgggttt tggagttgat gatggcggca
      181 agttttttgg aatgcaagga agagagccgg aaataatctg agtcatagcc ggcatcggcc
      241 aaaagaaata gcatcctggg tttaacctgc gcacggacag aagttttctc attgagaatt
      301 gaactaggct gccactttac cagcccccga gtgggtttaa gagtgtgcca ggtcttagcc
      361 aggtagagat attccagcaa atcctccttg tctcgatagc gaaccaggag cggtggctta
      421 tgggcgccca gactatcaaa tgctccactg ctgcgcccca tttcaaatcg ctgtccatag
      481 ctatcgccgt aaggattgac attctcaggg tcggtggccc ccagttctat gaggtttgcc
      541 gtggacgcta tgtcacgacc ggaagcatcg agaaggcggt acggcactac atagagtccg
      601 ctttgctgat agtccatatt cacttgatga gtaaagcctt taaactcctt acccagactt
      661 tcatcggtaa ccggttgaac agatacgggt accttcaccg gaatgaaatt ctcgttcaca
      721 tagttgtaga tttccggctg gcaaagtccg tcagactcaa ggcgataggc aaattcatcg
      781 cctttataaa gcaccgcata caaaagtggc tttctggtca gcttagcttc gctgattgcc
      841 tgggtgcggc tgcgccactt aataggcggc tgcttgcgaa atggtgaaga gagatcgtaa
      901 gtaatattca agccgaactc tttgtctacg cagagaaaag aagccaacat gaatgaacag
      961 ccggcaacaa gatagagcaa caaaggctgc ttgcgagtac ccttgtattt agtcctggcc
     1021 atatgagaac tccttgcggc tgaggaaaaa tatggctagc gacagacaaa cagtcaccac
     1081 acaaacaaaa tttatggtgg atgtaaaaat ggcgctgctg ccgttcaaga tttcggaaac
     1141 aggaatgttg ggaaagaaaa acacagcgaa accactgccg acaacatttg tcagatgagt
     1201 catgaactgc tgaccggctg ttcccgcgcc tcgcatacaa acttcgctta taagtatcgg
     1261 tcgaagaaac tgcatgccca ccatgccggc gaagagagta ccgacgatca aagccagatc
     1321 tttcaagccg gtaacaagag atgagtagaa gatgagcatg ctggccgtgc caaaagcaag
     1381 gatgacacgc tcaagaccgt tggccacaaa atcgaggtta cttagcagag gcggatgcaa
     1441 gattacggaa acggcatgct ccacaaacaa gcattgcaaa gagcagaggg aagcagcaaa
     1501 acccaccgcg aaccatttgc tcagtacata gcgactgacg gtcaggggtc gggccagaac
     1561 cagtgagatg gtgccgtgct gcctctcgca actgatgacg ccgacacccc agatgagcgc
     1621 aaacagactg gccacaaagg gataggaaat catatcttcc atgaggtcgt actcgtaagt
     1681 acagcgcctc caggtaaagt gagccagggg agccaatata agcaaggtga ggagaatcaa
     1741 cctgacatta ttggacttga actgccgcca ggtattaacg caaatagctc ggttcactgt
     1801 ttttctcctc tgcgcatttc gtcgataaag acccgttgca acctggacaa ttcaggcaag
     1861 actcgtgtta ccggcacgcc ggcttccact aaacctttaa tgagactggc actgtcttct
     1921 tcattgtcga caaaaaccct ggccagcccg tcttccgggg aaaattgttc aagggtggcg
     1981 ccacaatgag cagttaccct ggtaaaagcg gaagcttcca caggagcccc gtccaaaagc
     2041 cacttaataa tgagggtgtg gctcaagctg ctttcacgat cgcgt
//
LOCUS       DAOQNZ010000765         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35113_length_2082_cov_4.024667, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000765 DAOQNZ010000000
VERSION     DAOQNZ010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35113_length_2082_cov_4.024667"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..274)
                     /locus_tag="PKW73_13085"
     CDS             complement(2..274)
                     /locus_tag="PKW73_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74272.1"
                     /translation="MLLPIILLQNCETGEKTVYDRRDKFMASFKGGQWAENLLFADAE
                     LSVFVLSQDDAEIEKAAGEARLALGRPLVIPAESSDSSVNSAESTP"
     gene            497..581
                     /locus_tag="PKW73_13090"
     tRNA            497..581
                     /locus_tag="PKW73_13090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:531..533,aa:Leu,seq:tag)
     gene            716..1375
                     /locus_tag="PKW73_13095"
     CDS             716..1375
                     /locus_tag="PKW73_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74273.1"
                     /translation="MPENTDGNEKYPYFVFAKLVEQLLPDERERLYAAPLTAFLEERK
                     LGTVTGGGCQLAERAQPGEKDIDYAGLDIELANLDEALAFTKSKLTELGAPVGSEVEY
                     TGKDGETHSDPIGDLELLNIFIDNVSLAPEIYKNADMRTLCEGIESKLNADSLGELRG
                     PCVWPSEVLLYIVGGDADDIFAKIEPMRKDFPVLQNARVVFQRRDQKKAPKEIRIGFN
                     D"
     gene            complement(1499..>2082)
                     /locus_tag="PKW73_13100"
     CDS             complement(1499..>2082)
                     /locus_tag="PKW73_13100"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74274.1"
                     /translation="NLLLIQKRYQEAIQVCDAGLKVKDPSSTCFGNKAAALSSLNRHE
                     EALAVSVLYIAACVDQQTLIAALEAKAGILENLRRYRDALAVYKQVDAKSHSDSYLFK
                     QVDCLEKLGQSDEAVRVLTVIIARNPRDEDALARRARVHGRKGHLKEAEADYTRALAE
                     LKTSSFLKERAEIYKRMGRHDLYLSDLKAAEQL"
BASE COUNT          543 a          548 c          511 g          480 t
ORIGIN      
        1 atcagggcgt agattcagcc gagttcacac tcgagtcact gctctcggcc ggaataacca
       61 ggggacgacc aagggccagc ctggcctctc ctgccgcctt ctcgatttca gcatcgtcct
      121 ggctgagcac gaagacgctc agttccgcat cagcaaaaag cagattctct gcccactgtc
      181 cacctttgaa cgaagccatg aacttgtcgc ggcggtcgta gaccgtcttc tcgccagtct
      241 cgcagttctg caaaagtatt atgggcagca acataaatca gtctcatcag tgcgccggaa
      301 atgctcaact aagtctacat tacaagcgca ttgaaattgt gcacaagttt ccaagtcacc
      361 caaaggtacc cacctgcctg gaccgacctg gtaatcaagc gcaagccatg taagaagact
      421 gcaacactcc caaaggcggc caacgcccct gaagggtgct gtggtattct agaaaatccg
      481 gtatccaccg gtccaagggc gagtggcgaa attggcagac gcgctagact taggatctag
      541 ttcagcaatg agtggaggtt caagtcctct ctcgcccacc atttaaaatt ccgccgcaag
      601 gcggtttttt ttgagctgaa aaactgattg agacttccgc cggaagcctg tgtgtgggta
      661 gtcctagcac tggaaacgct accactgaag atcactaatt ctggagataa gattattgcc
      721 ggaaaatact gatggaaatg aaaagtatcc ctatttcgtc ttcgcaaagc tggtagaaca
      781 gctccttcca gacgaacgag aacggcttta tgctgccccc ctgacagcat tccttgaaga
      841 acggaaactc ggaaccgtca ctggtggcgg ttgccagtta gcagagcgag cgcagcccgg
      901 agaaaaggat attgattacg caggtctcga tatcgaactg gcaaatttag atgaagcgct
      961 ggcctttacc aaaagcaagt tgaccgagct tggggcaccg gttggctctg aggtggaata
     1021 cactggaaag gatggcgaga cccattcaga tccaattggc gacctagagc tactcaacat
     1081 cttcatcgac aatgtatcgc tggctccaga aatatacaag aatgcagaca tgagaactct
     1141 ctgcgaaggg atagaaagca agctgaatgc tgatagtctt ggagaattaa gaggaccgtg
     1201 cgtctggcca tccgaagtgt tgttgtacat tgttggaggg gatgccgacg acatcttcgc
     1261 aaaaattgag cctatgcgaa aagactttcc agtcctgcaa aatgctcgcg ttgtctttca
     1321 gagaagagac cagaagaagg cgccaaaaga aatccgcatt ggcttcaacg attgatagaa
     1381 agataaccca acgagatccg ctcgcttcga ttgtttcgat cacggacaat tattgccacc
     1441 agcttacagt acggaatgcc gagtcacccg tctcactttg ctaaaaacgt ccaggcttct
     1501 acaactgctc ggcggctttc aggtccgata ggtagagatc atgccgcccc atgcgcttgt
     1561 aaatttcggc gcgctccttg agaaaggaac tggtcttcag ctccgccagg gctcttgtat
     1621 agtccgcttc ggcttctttc aaatgtcctt tgcgcccatg aacccgagcc cgcctcgcta
     1681 acgcgtcttc atcccgggga ttcctggcga taatgactgt gagcacgcgc acagcttcgt
     1741 cactttgacc aagcttttcc agacaatcta cctgcttaaa gagataactg tcactgtggc
     1801 ttttggcgtc cacttgtttg tagacggcca gcgcatccct gtacctgcgc aaattttcca
     1861 gaataccagc cttagcttcc aaagctgcta tcaaggtttg ctggtccaca caggcagcta
     1921 tataaagaac agaaacagcc agagcttcct catggcgatt aagactgctc aaggccgcgg
     1981 ctttgtttcc aaagcaggtg cttgaagggt ccttcacctt aaggccggca tcgcaaacct
     2041 gaatggcctc ctggtagcgt ttttgaatca gcaaaagatt cg
//
LOCUS       DAOQNZ010000766         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35240_length_2078_cov_3.149778, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000766 DAOQNZ010000000
VERSION     DAOQNZ010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35240_length_2078_cov_3.149778"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..368
                     /locus_tag="PKW73_13105"
     CDS             24..368
                     /locus_tag="PKW73_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74275.1"
                     /translation="MSDLILASIVLPLSFVAGYLAARIRDGRTASPSVESEIVILLDN
                     LRQLERRSTNCDAGMRDFLSKARELIFLAIEEYRAPCLTNNRPVYFRRALISEYLMKA
                     RQELALAKTFEE"
     gene            379..1080
                     /locus_tag="PKW73_13110"
     CDS             379..1080
                     /locus_tag="PKW73_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74276.1"
                     /translation="MYNPVVFCGLLVLVILTWLICRKYFPFKINRTATRGNLAESQRQ
                     ELSRVVAEMSSEIKKASAAIGGAVQSYSGVVSAEAAVAEQIALFLSASAVGGFQGQER
                     KLAISARIAQTQAELRVKCAACREAIGTSVQSSESLDLALRALKNTVALDARQIDLLS
                     ELLATAQALLQVIEICHTCIRPAGLAASLIAVEAERLVVPVVPDKGLIADQLRAKPEE
                     TIHRRFSYGALPPLP"
     gene            complement(1161..1349)
                     /locus_tag="PKW73_13115"
     CDS             complement(1161..1349)
                     /locus_tag="PKW73_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74277.1"
                     /translation="MENEKASEQAPPLRADEQAADLARQALTLYECGPGGPIVTNPAD
                     CKDYSASNILPKLNLSDS"
     gene            complement(1522..>2078)
                     /locus_tag="PKW73_13120"
     CDS             complement(1522..>2078)
                     /locus_tag="PKW73_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74278.1"
                     /translation="EQTVFLSGSPTGSIIEKALGEYRRQKERSIDAMAATETFGEAEE
                     LELAPKTSPIEALDGGWDMFETLRLLTGRDYGAAQLTARPQPDKPWQISHEQFAHYLS
                     EHLAKGNGGALNLTMLRRTPASESYEHAYSLLDFDAAGKDGGTVTIRDPHGGFEDGIK
                     KYSVMELRELKAYVFGPIDTAGGK"
BASE COUNT          413 a          549 c          563 g          553 t
ORIGIN      
        1 ccttctgaaa ctagaggtta cctatgagcg acctaatcct ggcatcaatt gttttaccac
       61 tctcttttgt ggcgggctac ctcgctgctc gcatccgaga cggaagaacc gcatctccaa
      121 gtgttgaatc ggaaatcgtt attttactgg ataacctccg gcaactggag cgccgttcaa
      181 ccaactgtga tgcaggcatg cgtgactttc taagtaaggc tcgcgagctt atattcctgg
      241 ccatcgagga ataccgcgcc ccctgtctca ccaataaccg tcccgtctac tttcgccgcg
      301 ctcttatcag tgagtacttg atgaaggctc ggcaagagtt ggcactagcc aagacttttg
      361 aggagtaatc gaatgtacat gtacaatcca gtcgtctttt gtggtttgct cgtgctggtc
      421 atcctgacct ggttgatctg ccgcaagtat ttccctttca agataaaccg gaccgcgact
      481 cggggcaatc ttgccgagtc gcagcgacaa gaattgagca gagttgtcgc cgaaatgagt
      541 tcggaaatta agaaagcttc tgccgccatc ggcggtgcag tccagtccta tagcggggtg
      601 gtctctgcgg aagcggcagt agctgagcaa attgccttat tcctctctgc ttccgctgtc
      661 ggaggctttc aaggacaaga gcgcaagctt gccatctccg cacgcatcgc ccagacccag
      721 gcggagttga gggtgaaatg cgctgcttgc cgggaagcta tagggacctc ggttcagtct
      781 tctgagtctc tagacctggc tttgcgggct cttaaaaata ccgtagccct tgacgccagg
      841 cagattgacc ttctctctga actgcttgca actgctcagg ctcttttgca ggttatagag
      901 atttgtcaca cctgtattcg tcctgccggc ttagcggctt ctctcatcgc tgtagaggcg
      961 gagcggcttg tcgtcccagt ggtgccggac aaaggcttga tagcggatca attgcgtgct
     1021 aaacctgagg agacgattca ccgccgcttc tcctacggcg cgcttcctcc acttccttaa
     1081 actctctccc aacgtgaggc gcagacgcag aattgcgcct cacgttactg tttggtcggc
     1141 gctttgaccg agactttagc ctaggagtcg ctcaggttta gtttcggtag aatgttggag
     1201 gcgctatagt ccttgcagtc ggcgggattg gtgactattg gtccgcccgg cccgcactcg
     1261 tagagtgtta aagcttgtct tgcgaggtcg gcggcctgtt catcggccct gagcggcggg
     1321 gcctgctctg atgctttttc attttccatt tgtttatacc tcagaaggaa gagttgtttg
     1381 cagatagtct agttccgaca gctccgttga gtaagaaacg gaggtcatca gaagttagtt
     1441 acttttgtca cagtgctgcg aacctggtcg tcctttaagg gcagttgcac acgcgctttt
     1501 gcgcggcacg cagggctgta tttatttgcc accagcggta tcaatgggtc cgaaaacgta
     1561 ggctttcagt tcacgcagct ccatgacgga atattttttg ataccatcct cgaagccgcc
     1621 atgtggatct ctaattgtga cggtaccgcc gtctttgcct gcggcgtcaa agtccagtag
     1681 tgagtacgcg tgctcatagg actcagacgc aggggtacgc ctcaacattg tcaggttgag
     1741 ggcgccgcca ttgccttttg caaggtgctc gcttagataa tgagcgaatt gttcgtggga
     1801 gatttgccag ggtttatctg gttggggtcg tgctgttaat tgcgcggcac cgtagtctct
     1861 tcctgtgagg agccgaaggg tttcaaacat atcccagccg ccgtccaggg cctcgatggg
     1921 agatgtcttt ggtgccaact ccagttcttc tgcttccccg aaggtctccg tggcagccat
     1981 ggcatcgatg gaacgctcct tttgcctgcg atattccccc agcgctttct cgataataga
     2041 gccggtcgga ctccctgaga gaaagactgt ctgttccg
//
LOCUS       DAOQNZ010000767         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35340_length_2075_cov_3.506436, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000767 DAOQNZ010000000
VERSION     DAOQNZ010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35340_length_2075_cov_3.506436"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="PKW73_13125"
     CDS             <1..1488
                     /locus_tag="PKW73_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA74279.1"
                     /translation="THHNHISEEVAGQFTKEVVWHDKGETGKMDLIVDLNFRMDTSAL
                     YSDIVLPTATHYEKYDIATTDMHSFIHPLQPAVPPCWESKSDWEIFKGIALTTENLAK
                     QHMQEPIKDIIASPLMHDTPAELAQPTVQDWLKDECQPIPGKTMPNFKVVTRDYTKVY
                     QRFISLGPNFRNNGLAVHGTHYDVDDAYDAYLQSNPTVTFDGKTYPSLEHDREVCNAI
                     LHFAAETNGEMAYRAFQSESVKTGIDHTHLAADTRAVRMTIDDLKAQPRRVLTTPYWT
                     GITNGERTYSAYCQNIEEEIPWRTLTGRQHMYLDHETYIAYGEALPTYKPRPDVHLTK
                     DLEKTARDSGSLVLNYLTPHGKWHIHSTFGDTLRMKTLSRGIEPVWLNEKDADLIGVE
                     DNDWVEVLNDHGVVVTRACVSARIPRGLAFIYHSPERTATTPKSKERKNRRAGGHNSL
                     TRTRLKPLFMVGGYAQFTYAFNYWGPQGVNRDTYVVVKKMSQPEF"
     gene            1505..>2075
                     /locus_tag="PKW73_13130"
     CDS             1505..>2075
                     /locus_tag="PKW73_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74280.1"
                     /translation="MDVRAQVSMVFHLDKCIGCHTCSIACKNVWTDRAGAEYMWWNNV
                     ETKPGTGFPTLWEDQNHYKGGWTWKAKGNGKGRLQLRSQNKFDSFIKLFYNPNQPGLE
                     DYYEPWTYKYQELFDAPEGDDQPTAVPISQITGEPMDINAGPNWDDDLSGSPLYAEND
                     LNLDALSEEEREQLFEIERLFMMYLPRICN"
BASE COUNT          554 a          629 c          505 g          387 t
ORIGIN      
        1 acccaccaca atcacatttc ggaagaggtg gccggccagt tcaccaagga agtcgtctgg
       61 catgacaagg gcgaaaccgg caagatggac ctgattgtcg acctgaactt ccgcatggat
      121 acctcggcac tctactccga tatcgtcttg cccacagcta cacactatga aaaatacgat
      181 atcgccacca ccgacatgca ctctttcatt catccactgc agccggccgt accgccctgc
      241 tgggaatcga agagcgactg ggaaattttc aaaggcatcg ccctcactac cgaaaacctg
      301 gccaagcagc acatgcagga gcccatcaag gacatcattg cttcgccctt gatgcacgac
      361 acgccggccg aactggccca acccaccgtg caagactggc tcaaggacga gtgccagccc
      421 atacccggca aaaccatgcc caatttcaaa gtagtgacca gggactacac aaaagtctac
      481 cagcgcttca tcagcctcgg acccaacttc cgcaacaacg gactggccgt acacggcact
      541 cactacgatg tggatgatgc ttacgacgct tacttgcaga gtaatccgac cgtcaccttt
      601 gacggaaaga cctatccctc gctggagcat gacagagaag tctgcaacgc catcctgcac
      661 ttcgccgccg aaaccaacgg cgagatggcc taccgggctt tccagtcgga gtcagtaaaa
      721 accggcatcg accacaccca tctggccgcc gatacaagag ccgtgcgcat gaccattgac
      781 gatctgaagg cccagccaag gagagtcttg accacccctt actggaccgg tatcaccaat
      841 ggtgagagga cctattcggc ctactgccag aacatcgagg aagaaatacc ctggcgcacc
      901 ctcaccggca ggcagcatat gtacctggat catgagacgt atattgctta tggcgaggct
      961 ctgcccacct ataaaccacg gccggatgtg cacctcacta aagacctgga aaaaactgcc
     1021 cgggacagcg gctcccttgt tctcaactac ttgactcccc acggtaagtg gcacatccac
     1081 tccaccttcg gcgacaccct gcgcatgaaa actctctccc ggggtatcga accggtctgg
     1141 ctcaatgaaa aggacgcaga tctgatcgga gttgaggaca acgactgggt agaagtttta
     1201 aacgatcacg gcgtggtggt caccagagcc tgcgtgagtg cacgcatacc acggggactg
     1261 gccttcatct atcactcacc ggagcgcacc gccaccactc ccaagtcgaa ggagcgcaaa
     1321 aatcgccgcg ccggtggtca taacagcctc accagaacac gcctgaagcc cctcttcatg
     1381 gtgggcggct atgcgcaatt tacctatgcc ttcaactact ggggaccaca gggagtaaac
     1441 cgcgacacct atgttgtagt caaaaaaatg agccagccgg aattttaaaa ggaggtgaga
     1501 gtcaatggat gtcagagctc aagtatcaat ggttttccat ctggataagt gcataggctg
     1561 tcacacctgt agcattgcct gcaaaaacgt ctggacggac agagccggcg ccgaatacat
     1621 gtggtggaac aatgtcgaaa ccaagccggg cacaggcttt cccactctct gggaagacca
     1681 gaatcattac aaaggcggct ggacctggaa agcaaaaggc aacggcaaag gccggttgca
     1741 actgcgcagt cagaacaagt tcgattcctt catcaagctt ttctataatc ccaatcaacc
     1801 tggtctggaa gattattacg aaccctggac ctataagtac caggaacttt tcgatgcccc
     1861 cgaaggcgac gatcagccca ccgccgtacc gatttcacag atcaccggcg aacccatgga
     1921 catcaatgcc ggtcccaact gggacgacga tctttccggc tcgccgctct acgcggaaaa
     1981 cgacttaaac ctggatgcgc tctcagagga agagcgggag cagctctttg aaattgaacg
     2041 gctcttcatg atgtacctgc cgcgcatctg caacc
//
LOCUS       DAOQNZ010000768         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35396_length_2073_cov_4.418236, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000768 DAOQNZ010000000
VERSION     DAOQNZ010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35396_length_2073_cov_4.418236"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKW73_13135"
     CDS             complement(<1..295)
                     /locus_tag="PKW73_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA74281.1"
                     /translation="MLSAAPLADGRAEPLSPPVVAVRNLRRDYGAIKAVKDISFEIQE
                     GEIFGLIGPDGAGKTTTFHILGGVLEASGGQALTMGGKPRDMRLQIGYLTQQFS"
     gene            complement(299..1714)
                     /locus_tag="PKW73_13140"
     CDS             complement(299..1714)
                     /locus_tag="PKW73_13140"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA74282.1"
                     /translation="MIDKQYIPVGMICVVPGELPAGKSKSAFLTTRGAQMATETMTDP
                     GTQTGRSHGKPPAALVLVVILLVLGALGFYLYPRLFPAPEPDNRISVSGRIEGYETNV
                     GPKIGGRIDYVGFREGQSVKVGDQLIKLSDDEIKARLRGVKAKKQRAQEMVEESRYQI
                     TVVERQLEESNLRLTQSKEDTVAKVNEAEGNLAQAQARLAEAEAQLTQAKSELQLAKI
                     RKQRYEFLASRGAVTTDENDQAKTTCDSQEALVRSRESAVQAAIKQCHVAQSSLEQAR
                     TTRLNPGMMKAQLGVLEGQLAQARFKLKSAEHEAASAQADEDEVLANMAYLDIKSPIE
                     GIVTARAVEPGAVVVPGQTLMSLINLDTVYLRGYVPEGKIGKVRVGQTAKVVLDSAPD
                     KPFGGKVIQIDPEGSFTPENIYFKDDRVKQVFGIKIGIDQPDRFAKPGMPAEAVIELD
                     GSGEVGGGSGLPSNTLKNSGK"
     gene            1754..>2073
                     /locus_tag="PKW73_13145"
     CDS             1754..>2073
                     /locus_tag="PKW73_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA74283.1"
                     /translation="MNVQDANSTAELSTPRRGKREQIVQAAVKTFLEHGYAGTSMNKV
                     AEAAGVIKATIYSHFKDKQQLFIAIIEEMTIKKVAIDFTNPEPILSLEPDEFIDVLTG
                     KFCVL"
BASE COUNT          461 a          630 c          492 g          490 t
ORIGIN      
        1 gtgaaaactg ctgggtcaaa taaccaatct gcaagcgcat atcccggggt ttgcctccca
       61 tagtcaaagc ctgtccaccg ctggcttcca gcaccccgcc caggatgtga aaagtagtag
      121 tcttgccggc accgtcaggg ccaatcaagc cgaaaatttc gccttcttga atttcaaaac
      181 tgatatcttt caccgctttg atggcgccat aatcacggcg caaattccgc actgccacca
      241 ccggcggcga cagcggctcc gctcttccat cagctaacgg tgcagcactt aacacttttc
      301 attttcccga atttttcaag gtattggacg ggagaccaga cccaccgccg acctcaccgc
      361 tgccatccaa ctcgatgacg gcttcggcgg gcatgcccgg cttggcaaaa cgatcaggtt
      421 gatcgatgcc gattttgatg ccgaagactt gcttgactcg gtcgtcttta aaataaatat
      481 tctcaggcgt gaaagaacct tccggatcga tttgtataac cttgccgcca aaaggcttat
      541 caggagccga atccagcacc acttttgcag tctggccgac cctcaccttg ccaatttttc
      601 cttccggcac atagccgcgc aagtagacgg tatcaaggtt tatcaaagac ataagggtct
      661 gccccggcac caccaccgct cccggctcca ccgcccgggc tgtgacgatt ccctcgatgg
      721 gacttttgat atctaaatag gccatattgg ccagcacttc atcttcatca gcttgagccg
      781 atgctgcttc atgctcggca ctcttcaact taaagcgagc ctgagccaat tgcccttcca
      841 atacccctaa ttgcgccttc atcatgcccg gattcaagcg agtggtgcgc gcctgctcca
      901 ggctggactg agccacatgg cactgcttaa tagcagcctg cacggcactt tcccgcgacc
      961 gcaccagcgc ttcctgggaa tcacaagtag tcttggcctg gtcgttctcg tcggtagtaa
     1021 cggcaccacg gctggccaga aattcgtatc tttgtttgcg tatctttgcc agttgcagct
     1081 ccgacttggc ctgggtcagc tgcgcttccg cctcagcaag ccgtgcctgg gcctgggcca
     1141 aattgccctc agcctcattg accttggcca ccgtgtcttc cttagactgg gtcaagcgca
     1201 gattggattc ctccaactgc cgctccacca cggtaatttg atagcggctc tcttccacca
     1261 tttcttgcgc tctctgcttc ttggccttga caccgcgtag ccgcgcttta atctcatcat
     1321 cactgagctt tatgagctgg tcgccaacct tgaccgactg cccctcgcga aaccctacat
     1381 agtcaattcg tccgcctatt ttgggaccga cattggtctc atatccttca atacgaccac
     1441 tgacggaaat gcggttatca ggttcggggg cgggaaacaa gcggggatag aggtagaagc
     1501 ccagagcccc cagcactagc agtatcacca ccagaacaag agcggcggga ggcttgccgt
     1561 gcgaccttcc cgtctgcgtt cccgggtctg tcattgtttc cgttgccatc tgcgctcccc
     1621 tggttgtgag aaacgccgac ttgcttttac cggccggcaa ttctccgggt accacacaaa
     1681 tcataccaac cggtatgtat tgtttgtcaa tcatttttgt cgtaaaattt ttgcagtccc
     1741 ttgtgagaat cagatgaacg tccaggatgc caacagtacg gccgaactga gcacgccccg
     1801 ccggggcaag cgagaacaaa tagtacaggc ggctgtaaag accttcctgg agcatggcta
     1861 tgccggcacc agcatgaata aggttgccga agcggccggg gtgatcaagg ccaccatata
     1921 cagccacttt aaagataaac agcaactttt cattgccatc atcgaagaga tgaccatcaa
     1981 gaaggtggcc attgatttca ccaaccccga gccgatctta agcctcgaac cggatgaatt
     2041 catcgacgtg ctcaccggca agttctgcgt gct
//
LOCUS       DAOQNZ010000769         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35457_length_2071_cov_4.758433, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000769 DAOQNZ010000000
VERSION     DAOQNZ010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35457_length_2071_cov_4.758433"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKW73_13150"
     CDS             complement(<1..486)
                     /locus_tag="PKW73_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74284.1"
                     /translation="MARPITLLAVIALLVSWQAVSAAPESPNKQITLDFPDRYLYGWV
                     GHVDWHTDKLAKKLKWSDQISARGKVIINQGSGLIFEGTGSICLHPEVLDKLPADAFV
                     NMVFDNTEFDDSGAVRLGRFKMLERLSLEQTEVTDKIIDVVSGMPRLKLLFISRTSVK
                     GK"
     gene            complement(487..1542)
                     /locus_tag="PKW73_13155"
     CDS             complement(487..1542)
                     /locus_tag="PKW73_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74285.1"
                     /translation="MTRKPSNSAESDDTKASPQSLSLALDTVAENEKDKPQKPLVDMA
                     SPLDTMKVGQTRDWLKEAEHLPSLHLTDAGETTRKTIMIDGVEREYYLHVPKNYSPLN
                     PPPVILALHGYGYNTGQGGSEHGGKGFEQVSDFSKLADHENVIVIYPSGNQEQNFGWN
                     NGQWWFSKLDDVKFIDHLLNKTARDFPLDQSRIYAIGYSQGGSFLHRLIDELPNRFAA
                     AAEVGGWMSGAEGKGAKGLPFMAIQAVVDPTVPPDGRLLGLRMQSEDYTSNYYRAMNG
                     ITENPLDTARRVKHNGLVRELTSKIPGQPGEVKLMWLNNEVNHSWYGGLGDKEASINA
                     TNETWNFVSRFRKGQPP"
BASE COUNT          452 a          513 c          559 g          547 t
ORIGIN      
        1 cttgcctttg accgatgtgc gacttatgaa aagaagtttg agccggggca taccggaaac
       61 cacgtcgatg atcttgtcgg taacttctgt ctgctccagg gatagtcttt caagcatttt
      121 gaaacgaccg aggcgcacgg ctccgctatc atcaaattcc gtgttgtcga agaccatatt
      181 gacaaaagca tcggccggta gtttgtccag aacttccggg tggaggcaga tgctgcctgt
      241 gccttcgaaa atgagaccgc taccctggtt gatgattact ttgccccgcg cacttatctg
      301 gtccgaccat ttcaattttt ttgccagctt gtcggtatgc cagtcaacat gacctaccca
      361 gccgtataga tagcggtcgg gaaagtccag agtgatttgc ttgtttgggc tttccggcgc
      421 agcgctaacc gcctgccagg agacgaggag agctataaca gcaagcagtg tgatggggcg
      481 agccaattaa gggggctgtc ctttcctgaa gcgggatacg aagttccagg tttcatttgt
      541 ggcgttgatg gaggcttcct tatctccgag acctccatac caggagtggt ttacttcatt
      601 attcaaccac ataagtttga cttcgccggg ctgaccagga atctttgagg tcagctctct
      661 taccagacca ttgtgcttga ctcgcctggc cgtatccagc ggattttccg taatgccatt
      721 catggcgcgg taataattgc ttgtgtaatc ttccgattgc attctcagcc caagcagacg
      781 accatccggc ggtaccgttg gatcaactac ggcctggata gccataaacg gcaagccttt
      841 ggcgccttta ccttcggcac cgctcatcca gccccctact tcggcggcgg cggcgaaacg
      901 attgggcaac tcgtcgataa ggcggtgcag gaagctgccg ccctgagaat agccgatggc
      961 atagatgcgg gactgatcga gggggaagtc tctggctgtc ttatttagca ggtggtctat
     1021 aaatttgacg tcatccagct tgctgaacca ccactggccg ttattccagc cgaagttttg
     1081 ctcttgattg ccggaaggat aaatgacgat gacattctca tggtcggcca gtttactaaa
     1141 atcggagacc tgttcaaagc ctttgccgcc gtgttcgctg ccgccctgac cagtgttgta
     1201 gccgtagccg tgcaaggcta atataaccgg cggtgggttg agaggactgt agttcttggg
     1261 cacatgcaag taatattcgc gctcgacgcc gtcaatcata atggttttgc gagtagtctc
     1321 gccggcatca gtgagatgca acgagggcag atgctctgct tctttcagcc agtctcgcgt
     1381 ttgtccgact ttcatcgtat cgagggggct ggccatatct accaggggct tttgcggttt
     1441 gtctttctca ttctccgcaa ctgtatccag agcaagcgac aaagactgcg gcgatgcttt
     1501 cgtatcgtca ctttcggcgc tgtttgaagg ttttctagtc acggtgatgg cggatttgca
     1561 tgcagtcgag ttctctcatt attcccggtt gagttttcgc taaactgtgc gctgtcgccg
     1621 ttcttgatgt catggacggc tgtcagtttg gcgagaggaa caagcagaag ccgcgctaaa
     1681 gacaattttg ggcggtaaat tcgtcactct gccaggagca ggcgctcaga aaagaaagag
     1741 cgcattgctt acctgtggag cacgttggca agtgtaggta gtgggccgat ttacttgggg
     1801 ggggctgggt cagcgccagc tttcacctga atagttgagc tttgcagcaa atcgacaaac
     1861 aacgggaatt gttgatttga cggaagcagg cgctattttt gttcgaatcg taagatgacg
     1921 ccaattgtct tgctggttgc aaagctggaa tgcaagcact ctatcgccga tggtcaggta
     1981 gtcactatct tcatttgttt caacagcaac ccttccaaac acttacccgg taagtaacta
     2041 caagcaagca atcagctcgg ctttgccgtt c
//
LOCUS       DAOQNZ010000770         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35487_length_2070_cov_3.950868, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000770 DAOQNZ010000000
VERSION     DAOQNZ010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35487_length_2070_cov_3.950868"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKW73_13160"
     CDS             <1..933
                     /locus_tag="PKW73_13160"
                     /inference="COORDINATES: protein motif:HMM:NF037014.2"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-diphosphate and nicotinate from nicotinate
                     D-ribonucleotide and diphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNA74286.1"
                     /translation="AGRLYGIPVKGTHAHSWVMSFPTEAEAFHAYAEAMPNNCVFLVD
                     TYDTLHGVKTAIEQGHWLKENGHDLVGIRLDSGDLAYLSAEARQLLDRAGFADAQIIA
                     SNDLDEQVIESLIDQGAMVDVWGVGTKLITAADQPALGGVYKLACVRECNLQDGTTKP
                     WQYRIKLSEQVVKISTPGVQQVRRFFSQSESGTKTFIADMIYDQESQAEPENLVVDPL
                     DFTRRKPVAAGTAYEELLLPIFRGGRCVYSMPGLEESRSFREERLQGFHRGILRLLNP
                     HQYPVGIEAGLSRAKLALIMKERGFNGESHEKLL"
     gene            complement(950..1288)
                     /locus_tag="PKW73_13165"
     CDS             complement(950..1288)
                     /locus_tag="PKW73_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74287.1"
                     /translation="MREVPLPFSDGLLSAKLWQSQTKDGKERFVAYVPELGLSCFGTS
                     DSEASFRLFSALIKYFRQLKAHKEKLGEKGLAHLQMLTVWMNAIEERMTAKQDHPYEG
                     RLIALRGRSR"
     gene            complement(1394..>2070)
                     /locus_tag="PKW73_13170"
     CDS             complement(1394..>2070)
                     /locus_tag="PKW73_13170"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HNA74288.1"
                     /translation="LISQGKVPIVCGGTGLYARVLLEGMNIPSVPPDEKLRAELQQLV
                     EEKGVEALHEILEKLDPDSLKRIQRNDVFRLSRAIEVSRALGRPFSQAAGRSDVPYDV
                     LWLFLNVDDRQVLRKRIIDRLQEQVKAGVVDETRHLLLRFGRSQSLVNAVPYKEYLQF
                     LDGQIDLDTAHEEAVKHNYQLARRQIMWFRANKNAINICVDKSSEKEQFEISMKEISL
                     KLALQK"
BASE COUNT          456 a          554 c          552 g          508 t
ORIGIN      
        1 gccggccgcc tctacggcat accggtcaaa ggtacccacg cccacagctg ggtcatgtcg
       61 tttcccacag aagcggaagc gtttcatgcc tatgccgagg ccatgccgaa caactgtgtt
      121 tttctggtgg atacctatga cactctccat ggtgtcaaga ctgccatcga gcaaggacac
      181 tggttgaagg agaacggtca tgacctggtc ggtattcgtc ttgattccgg cgaccttgct
      241 tacttgagcg ccgaagcccg gcaacttctc gatagagccg gttttgccga tgcccagatc
      301 attgcttcca acgacctcga cgagcaagtt atcgaatctc tcatagacca gggcgccatg
      361 gtagacgtct ggggcgtggg aaccaaattg atcactgctg ccgaccagcc ggccctgggc
      421 ggtgtctaca agctagcctg tgtacgggaa tgcaacttgc aggatggcac gactaagccc
      481 tggcaatatc gcatcaagct ctccgaacag gtggtaaaaa tttccactcc cggtgtgcag
      541 caggtgcgtc gcttcttcag tcaatctgag agcggcacca agacctttat tgccgacatg
      601 atctatgacc aggagagcca ggctgagccg gagaatctgg tggtggaccc tctcgatttc
      661 accaggcgca agcccgtggc tgcgggcacg gcctatgaag agttactgct gccaatcttc
      721 aggggcggcc gctgtgtcta ttccatgccc ggcctggaag agagcagaag cttccgagaa
      781 gagcgtttgc agggcttcca ccggggtatc ttgcgcctcc tcaatccgca ccagtatccc
      841 gtgggcatcg aggccggtct gagccgggcc aagctggcac tgattatgaa agagcgcggc
      901 ttcaacggcg aaagtcacga aaaattgctt tgaatttcaa actgaggagt tagcggctgc
      961 gacccctgag ggctatcaga cgaccttcgt atgggtggtc ctgtttagcc gtcatgcgtt
     1021 cttcgatggc gttcatccag accgtcagca tttgcagatg ggcaagacct ttttctccca
     1081 gcttctcttt atgagctttc aattgccgga agtatttaat cagcgccgaa aagagacgga
     1141 aggatgcttc cgaatcggaa gtaccgaagc aactgagacc aagctctggt acataggcga
     1201 caaaacgctc tttaccgtct ttggtctggc tctgccagag ttttgcggag aggagtccat
     1261 cactgaatgg cagagggact tctctcatgc cgcttgtttg ttttgacttc atcgtaggaa
     1321 cgagccaaga gaccttactc ttgttagtat agctcttaaa cggcaggcgt ctagactcag
     1381 atatgttgct agactacttt tgcagagcca atttcagaga tatttctttc atactgatct
     1441 cgaactgctc tttttcgctg cttttatcta cgcatatatt gattgcattt ttgttggcgc
     1501 gaaaccacat gatttgccgc ctggctagct gatagttgtg ttttaccgct tcttcatggg
     1561 cggtatcaag atcaatttgt ccatccagga actgcagata ttccttatat ggaacggcat
     1621 tcacaaggga ctggctgcga ccgaagcgca agagtagatg ccgggtttca tccaccacac
     1681 cggctttcac ttgttcttgc aggcgatcga tgatgcgctt tctcaggacc tggcgatcat
     1741 cgacattgag aaaaagccag agcacatcat agggcacatc gctacgcccg gctgcctgtg
     1801 agaaaggtcg accaagggcc ctgcttacct cgatcgcgcg actgagacgg aagacatcgt
     1861 ttctctggat gcgcttgagg gagtccggat ctagtttctc cagaatttca tgcagggcct
     1921 ccactccttt ttcttccacc agttgctgca gctcggccct gagtttctcg tccggcggta
     1981 ccgaggggat gttcatgcct tccagcagca ctcttgcata gagaccggtg ccgccgcaaa
     2041 caattggaac cttgccctgg gagataaggg
//
LOCUS       DAOQNZ010000771         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35571_length_2068_cov_2.925484, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000771 DAOQNZ010000000
VERSION     DAOQNZ010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35571_length_2068_cov_2.925484"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="PKW73_13175"
     CDS             complement(<1..2045)
                     /locus_tag="PKW73_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA74289.1"
                     /translation="MVEQRGLVNLLKEQIQAFSITEESRSLFYLSTSFDASISDIGTA
                     LLSGAALIIEKADKLRPGKELIELMKKRQVSYVDIPPSVLKLLDPTLCPPTLKSIVIG
                     GEVCPQDVVRAWAARVKLVCVYGPTEATVCTSLTHCGTDWQAPLIGAPIKNISYLVLK
                     EDGTPAEVGESGELFIAGIGLARGYLHRSDLNRERFLTTDGIRYYKTCDRVLVNASGN
                     HEFQGRIDRQMKLRGLLIEPEEIEARLCEHPQIARAAVLKRAVSSKSSREMLVAFLLP
                     SRLHESLPNARELRLHLSRSLPVWMLPQRYQWLETLPLTASGKIDLTALAACPLKEQA
                     SAPPISRTGASEDEEERIARCLCRILGYEQIDLEDDFFDLGLDSLGAVELAAALSVKG
                     LDLSPEAVFTHASCTKLARFCRNSNADHESADSGDVMEAAALTRRLEEWPKFQALLHS
                     GGKLAVTGASERKNRSGTLFTGATGFLGIRLLKELLEADQASNAVSWQPATLYALVRA
                     RNQAEATVRILNTAGRYLQDRQSLELLNSALSRGQLRPLPGNIEEERLGLEDCYEELT
                     EATDRVIHLAARVNMVLPYRSLEAANTEGTLNLAAFAMHGRLKDFHYASTLSVFVSTD
                     QNHGRLLESDDLSRTRLIWGGYAQSKWLAEVLLRKLGKRGLPLSIYRLGLITGDTTNG
                     "
BASE COUNT          400 a          594 c          604 g          470 t
ORIGIN      
        1 ccgtttgtgg tgtcgccggt tattaagccc aggcgataga tactgagagg cagaccgcgt
       61 ttgcccagct tgcgcaaaag cacttccgcc agccatttgc tctgggcgta gccgccccat
      121 ataaggcgcg tccggctcaa gtcgtcgctt tccagaagcc gcccatggtt ttgatcggta
      181 gaaacgaaga cggaaagtgt ggaggcatag tgaaaatctt tcagccgacc atgcatggca
      241 aatgccgcca gattgagggt gccttccgtg ttggctgctt ccagcgagcg gtagggcaag
      301 accatattca ctcgcgctgc caggtgaatt acacgatctg tagcctccgt cagttcttca
      361 tagcagtctt ccagaccaag gcgctcctct tcaatgtttc cgggcaaggg gcgcagttga
      421 ccccggctca gggctgagtt tagtaactcc aggctctgcc ggtcctgcag gtagcggccg
      481 gccgtgttca aaatgcgaac ggtcgcttcc gcctggttcc tggcccgcac cagggcatag
      541 agtgttgccg gctgccaact gacagcgttg cttgcctggt cggcctccag cagttctttc
      601 aaaagtctga tgccgagaaa gccggtggca ccggtgaaga gcgtaccgct gcggtttttt
      661 cgctcactgg cgccagtcac tgcaagcttt ccccccgagt gcagcagggc ttggaacttc
      721 ggccactctt ccagtcgcct ggtgagggcg gctgcttcca tcacatcgcc tgagtctgcg
      781 ctctcatggt cggcattact attgcggcag aagcgggcca gcttggtgca gcttgcatga
      841 gtgaaaacgg cctcggggga aaggtctaga cccttgacgg aaagggccgc cgccagttcc
      901 accgcgccca gggagtccag acccaggtcg aagaaatcat cctccaggtc tatctgctca
      961 tagcccagga tgcgacagag gcagcgggcg attcgctcct cttcgtcctc gcttgctccg
     1021 gtacgggaaa ttggcggcgc gcttgcctgc tctttgaggg ggcaggcggc cagagccgtc
     1081 aaatcaattt tgccgctggc ggtgaggggc agtgtctcaa gccactggta acgctgcggt
     1141 agcatccaga caggcaggga gcggctcagg tgcagccgca attccctggc attcggtaag
     1201 ctttcatgca agcgagaagg cagaagaaac gccaccagca tttccctgct acttttagag
     1261 ctgaccgctc ttttcagtac ggccgctcgg gctatttgag gatgctcgca gagcctggct
     1321 tctatttctt ccggttcaat gagaagaccg cgcaatttca tctggcggtc gatgcgacct
     1381 tggaattcgt ggttgcctga agcattcacg agcacgcggt cgcaggtctt gtagtacctg
     1441 ataccatctg tggtgagaaa gcgttctcgg ttcaaatcgc tgcgatgcaa ataacccctg
     1501 gccagtccta tgcctgcaat gaagagttcg ccgctttcgc ccacttctgc cggtgtgccg
     1561 tcttccttca aaaccaggta gctgatattt ttgatgggtg ctccgatcaa gggcgcttgc
     1621 cagtccgtcc cgcagtgggt caggctggtg cagaccgttg cttccgtggg tccatagaca
     1681 caaaccagct tgactctggc cgcccaggct ctgaccacgt cctgggggca gacctcgccg
     1741 cctattacta tcgacttgag ggtgggcgga cagagggtcg ggtcgagcag cttgagcaca
     1801 ctgggaggga tgtccacata actgacctgc ctctttttca tcagttcgat caattctttc
     1861 cccggtctca gtttgtcggc tttctcgatg atcaaagcgg ccccgctcag tagggcagtg
     1921 ccgatgtcgg atatggaggc atcgaagctg gtggaaagat aaaaaagaga gcggctctct
     1981 tcggtgatgg aaaacgcctg gatctgctct ttcagcaggt tgaccagacc tctctgctca
     2041 accatcactc ccttggggcg ccccgtag
//
LOCUS       DAOQNZ010000772         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35585_length_2067_cov_3.975646, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000772 DAOQNZ010000000
VERSION     DAOQNZ010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35585_length_2067_cov_3.975646"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..445)
                     /locus_tag="PKW73_13180"
     CDS             complement(68..445)
                     /locus_tag="PKW73_13180"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNA74290.1"
                     /translation="MNEKQFNDFCKKLPATTYVKQWGESHVWKVGGKVFAIGGWEDGD
                     SEEFAVTFKVSDMNFEILKERPGLRGAPYLASRGLKWIQHYGKPGLNDDGLKDCIKRS
                     YEMIARALSKKKQLELGIRVGSE"
     gene            complement(498..1298)
                     /locus_tag="PKW73_13185"
     CDS             complement(498..1298)
                     /locus_tag="PKW73_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74291.1"
                     /translation="MSDISRWSKPDPPSEKVHTEEAPVEEVAALQSAELAPESLTDAA
                     GKDEADLLSNSAVEESAQAEHDIPEGQEGPAPGTSDGCLSCEDAAVSEGDESEAEESS
                     APEADPLKQYVLAMSTSLFLIWLPFALNATGVLGGKYEFAGARAQAMVNIFTAIAVPA
                     SLIGIIVCGGKTKSLWASVGLAAFLVVALSVGGLPLAFKAAGTWQALMRDRDPNLQKL
                     NEEMVNARTRLVLYSYNPPLERTRRVLRAEEEVLPGILLIKLQAQKSD"
     gene            1417..>2067
                     /locus_tag="PKW73_13190"
     CDS             1417..>2067
                     /locus_tag="PKW73_13190"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNA74292.1"
                     /translation="MPGSMTTLIESNLRRAGRLLMDADAVLIGADAGMSVDSGLPDYR
                     GTRGFWTAYPAFAGRTYAEIAGPTLMQDKPEQAWAFFGHCFELYRKTPPHVGYFKLRE
                     TLEQLGKDYFVFTSNIDGFFRRSDYLCSSIYEVHGSINRLQCRRGLDCGSHTWSAEDL
                     YIEVDLEQMLATSPLPRCIKCEDFARPNVLLFGDRHWVPHAVIKEENLYEAWLAKLR"
BASE COUNT          524 a          576 c          489 g          478 t
ORIGIN      
        1 ttgacagtcg ggtcacgcct agatgatagg aatgaagatt ccgaagcgca accgaatgca
       61 tcgagagcta ctctgaacca actctgatac ccaactccaa ctgtttcttc ttcgataacg
      121 ctctggcaat catctcatat gagcgcttga tgcaatcctt cagcccatca tcattgagac
      181 cgggcttgcc atagtgctgt atccacttca gtccacgcga cgcaagatag ggcgcaccgc
      241 gcaagccggg cctttccttc aagatctcaa aattcatatc tgagactttg aaggtaactg
      301 caaactcttc actatcgcca tcttcccagc cgccaatggc aaagactttg cctcccactt
      361 tccagacatg agattcgccc cactgcttca cgtatgtggt agcaggcagc ttcttacaaa
      421 aatcattgaa ttgcttttcg ttcatcgact aatcctagca gaaaccctgc aacctgaaga
      481 ctcgccaaac aaggtactca atcacttttc tgagcctgaa gtttaatcaa tagaatgccg
      541 ggcaagactt cttcctcagc ccgcaagacg cggcgggttc gttccagggg cggattgtag
      601 ctatacaaga caagcctggt gcgcgcattg accatctcct cattcagctt ttgcaagttt
      661 ggatccctat ctcgcatcaa ggcctgccag gtgccggcag ctttaaatgc caggggcaga
      721 cctcccacgg aaagtgccac cactagaaat gcggcaagcc cgactgaagc ccataaactc
      781 ttagttttgc cgccgcaaac tattattccg atcagactgg caggaacagc aatggcagtg
      841 aaaatattca ccatagcctg cgctctggca ccggcaaatt catacttacc tccgagcaca
      901 ccggttgcat tcaaggcaaa aggcaaccag ataagaaaga gactggtgct catggccaga
      961 acatattgct tgagaggatc agcctctgga gccgacgatt cttcggcttc actttcatcg
     1021 ccttcggaaa ctgcggcatc ttcacaagac aagcatccat cagaagtacc gggcgccgga
     1081 ccttcctgac cttccggaat gtcgtgctct gcctgcgcac tttcctctac ggcagaattc
     1141 gacaagagat ctgcctcatc cttgcctgca gcatccgtga gactttccgg cgcaagctct
     1201 gccgactgca gagccgcgac ttcctcaaca ggggcttcct cagtatggac tttctcagat
     1261 ggtggatccg gcttggacca gcggctaata tcggacaaag caattaccat tcatcgatgt
     1321 aacaaaagct cggagtaagc ttcagaaatt atagcagcgc aaaagcagca gcaattgcac
     1381 gtgctacgct gggaagagcg acatgggggg aagccgatgc ctggatctat gaccaccctg
     1441 atagagtcta atttaagaag agccggtcgc ttacttatgg atgccgatgc cgtgctcata
     1501 ggggcagatg caggcatgag cgtcgactcc ggcttgcccg actaccgggg caccagaggc
     1561 ttctggactg cctatcctgc ttttgccggg cgaacctacg cagaaatagc cggccccact
     1621 ctcatgcaag acaagccgga acaggcctgg gccttcttcg gtcactgctt cgagttgtat
     1681 cgaaaaacac cccctcatgt cggctatttc aaactgagag aaacgcttga gcaattaggt
     1741 aaggactatt ttgtttttac aagcaatatt gacggcttct tcagaaggtc cgattatctg
     1801 tgctcaagta tttatgaagt gcacggctcc atcaaccgct tgcagtgtcg ccgggggcta
     1861 gattgcggca gtcatacctg gtcggcagaa gatctgtata tcgaggtaga cctggagcaa
     1921 atgcttgcca ccagcccctt acctcgctgc attaagtgcg aagattttgc cagacccaat
     1981 gtgcttctct ttggcgatcg acactgggtg ccccatgcag taattaaaga agagaacctc
     2041 tatgaagcct ggctggcaaa actaagg
//
LOCUS       DAOQNZ010000773         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35704_length_2063_cov_4.080179, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000773 DAOQNZ010000000
VERSION     DAOQNZ010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35704_length_2063_cov_4.080179"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="mutL"
                     /locus_tag="PKW73_13195"
     CDS             complement(<1..564)
                     /gene="mutL"
                     /locus_tag="PKW73_13195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNA74293.1"
                     /translation="MPKVFVLPDHIASQIAAGEVVERPSSVVKELVENSLDAGATEIE
                     VSVSADCRDLRIADNGSGMEPEDAVLAFQRHATSKLRSADDLYNLTTLGFRGEALPSI
                     ASISKFTCYTRTHTAPHGSRIESADGNLTAQEAGCAPGTVMEIKELFYNTPARLKFMK
                     KANTEFGHIEEILQSLAISYPHVAFTLI"
     gene            826..1221
                     /locus_tag="PKW73_13200"
     CDS             826..1221
                     /locus_tag="PKW73_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74294.1"
                     /translation="MEDFEHALQVDVLACSLRLDKDASGDLLEHLSAKLAQALPDECE
                     ITRGGWLLSSKKPVVELSVKLDDTGFQFTRDKRGVISARRQKIVRGVVLKSEDVEVEK
                     LIGELAEKLASLAEKNARTREALQKFVVG"
     gene            1316..>2063
                     /locus_tag="PKW73_13205"
     CDS             1316..>2063
                     /locus_tag="PKW73_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNA74295.1"
                     /translation="MPFWIFKEKDDDKAKRELQEASVKELEKGNLPLGAQHRLKAQAE
                     SGKNFFSSNLSAKEYLLAREAGYVPVGQVMGSAFMKVGWRNFCQGFWSSTSEISALTN
                     AHKQARELAVSRLRQEAELLGAHGVIGVKVTTSTFNWGEGITEFTAMGTAIRIPGSAC
                     VKPGHVPFTSTLSGQEFWQLYEAGFWPTGLVMGNCSYYIKGNQQTNSAQMGWFGRLAN
                     QELMVFSEGFAYARKQANQRLWVDIGLNGGD"
BASE COUNT          466 a          477 c          609 g          511 t
ORIGIN      
        1 gattaaggtg aaggccacat gggggtagct gatggccagg ctctgaagaa tttcttcgat
       61 atggccaaat tccgtattgg cctttttcat aaacttgagc cgggccggcg tattgtagaa
      121 aagctctttg atctccatga ccgtgcccgg ggcgcaaccg gcttcctgag ccgtgaggtt
      181 gccgtcggcg ctttcgattc tggagccgtg cggagccgta tgggtgcgcg tatagcaggt
      241 gaatttggag attgaagcga tggaaggcag cgcttcgccg cggaagccca gggtggtcaa
      301 attgtagagg tcgtcggcgc tgcgcaattt tgaggtggcg tgcctttgaa aggccagcac
      361 cgcatcttcc ggctccatac cggagccgtt gtcggcaatg cgcaggtcgc ggcaatcggc
      421 tgagacgctc acctcaattt cggtggcacc ggcatcgagg gagttttcca ccagctcctt
      481 cactacggat gaagggcgtt ctaccacctc tccggcggca atctggctgg ctatatggtc
      541 gggcaataca aatactttcg gcatggaagc atcttagcat ccactgggct cagaccattc
      601 aaagagactt tgagagctgt gtaatcttct tggaatggtg ttctaaccag ccggggcgcc
      661 tggttgtccg gctcaaggta gggccactat gtatagtcag tacgcctcac tttcactcgc
      721 caggcagtca agataatgtg tgctttcctt gagacctata taatgtggca attcaacgtt
      781 tatccgttgt tgtttgtttg ggaatcgcta tttaggaaag gtcaagtgga agacttcgaa
      841 catgctttgc aggtggatgt gcttgcctgc agtttgcgcc tggacaagga cgcttccggt
      901 gatttgctcg agcatctttc ggccaagttg gcccaggctt tgcccgatga atgtgagatt
      961 actcgcggcg gctggttgct gtcttccaag aagccggtgg tggagctttc cgtcaagctt
     1021 gacgacaccg gttttcaatt tacccgtgac aagcggggag tgatttcagc cagacggcaa
     1081 aaaatagtgc gcggtgtcgt actaaaaagt gaagacgtcg aagtagagaa actcatcggt
     1141 gaactggctg agaaattggc ttccctggct gagaaaaatg cccgtacccg ggaagcattg
     1201 caaaagttcg tggttggttg attgaagtgt tggttgattg agagtgtagc gacaatttag
     1261 taatcgtatt agcaagagtc gtctcaagtt tgactctgca agccggggaa aataattgcc
     1321 tttctggata ttcaaagaaa aagatgatga caaggctaag cgcgagctgc aagaagccag
     1381 cgtcaaggag cttgagaagg gtaatttgcc gcttggtgcc caacaccgtc tcaaagctca
     1441 ggctgagtcc ggcaagaatt tcttctccag caatttaagt gccaaagagt atcttctcgc
     1501 tcgtgaagcc ggttacgtgc ccgttgggca ggtgatgggc agtgctttca tgaaagtagg
     1561 ctggcgtaat ttttgccagg gcttctggtc ttccacatcg gagataagcg ccctcactaa
     1621 cgcccacaag caggctaggg aactggcagt gagccggctc aggcaagagg cggagttgct
     1681 tggtgcccat ggtgtcatag gcgtcaaggt gactacttca accttcaact ggggtgaggg
     1741 aatcaccgag ttcacggcca tgggcacggc cattcgtatt ccgggaagtg cttgtgtaaa
     1801 accgggacac gtgcccttca cttctacttt gagcgggcag gaattctggc agctctatga
     1861 agccggcttc tggccgaccg gcctggttat gggcaactgt tcctattaca ttaagggcaa
     1921 tcaacagact aattcggcgc agatgggctg gtttggcaga cttgccaatc aagagctgat
     1981 ggttttcagc gaaggctttg cctatgccag aaagcaagcc aatcaaaggc tctgggtcga
     2041 cattggtttg aatggcggtg atg
//
LOCUS       DAOQNZ010000774         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35721_length_2063_cov_2.490040, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000774 DAOQNZ010000000
VERSION     DAOQNZ010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35721_length_2063_cov_2.490040"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKW73_13210"
     CDS             <1..475
                     /locus_tag="PKW73_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74296.1"
                     /translation="LAKRRSEIVSTGSFVVSSIIPDEERLHLNESEGGETKPGDDHIN
                     VKWANKHLMRADELLKKKRFSEAETAMKEAIRLVPNEPRYHNKLAEVYLVRGWKTLAM
                     TEVQTALRLDGTNPEARSLEVQLKSLMKDNSAKGSAEGKKKGLMQQIKEILNKKI"
     gene            489..1766
                     /locus_tag="PKW73_13215"
     CDS             489..1766
                     /locus_tag="PKW73_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74297.1"
                     /translation="MIFQGSHSIRVRAAGFLILLCLLAKVGSGPVQASENARSKPSID
                     LELPDEPPLEMEEAASASDSSAGSGSNFTVPGTDLLAGTTGSADFSKRPVLKGSISGG
                     NNAGAPSPLLSGSLQMVPKGTTVDLTLQCHLNSELSQKGQEVFARISRDVTAEDGSKV
                     VLPGKWVAHGYVTRVDKQKHNGRDGFVEVKFDRIISPDGEWEVPFETSFSTKDNELKS
                     AAKVLFRDTKFATIGALAGSILSVQMTGLPVAISTYGISVGIGGGVGATYGLVCAMAR
                     KGKICSVYPGDHVQLKLSEPLSLPGFNPLALDSAKNSSHLPGFSLAVTDFKFEKDPVS
                     QDKRGQVLRLWLTAENRSRRSLNLRNLRVVSEMDDIYAPHLSAAMMRFQELAPGGRQS
                     LVLPFSVDSKKHKYSLVLLKDASGAELARAPIN"
BASE COUNT          491 a          503 c          562 g          507 t
ORIGIN      
        1 cctggccaag aggcgttccg aaatcgtctc cactggctct ttcgtagtca gctccattat
       61 tcctgatgag gaaaggctgc acttgaatga gagtgagggg ggcgagacca aaccgggcga
      121 tgaccacatt aatgttaagt gggccaacaa gcatttaatg cgcgctgatg aactgctcaa
      181 gaagaagcgt ttttctgaag cggagacggc tatgaaggaa gcaattcggc tggttcccaa
      241 cgaaccccgc taccataaca agctggctga agtttacctt gtcagaggct ggaagaccct
      301 ggctatgacg gaagtgcaga cggctttgcg tctggatgga actaatccgg aagcccgtag
      361 tctagaagta cagctcaagt cgcttatgaa ggataactcc gccaagggct cggcggaagg
      421 taagaaaaag ggtctcatgc agcagattaa ggaaatcctc aacaagaaga tttgaggagt
      481 gatgagtttt gattttccag ggcagccaca gtattagagt cagagccgca ggcttcctta
      541 tattgttatg cctgcttgca aaggtcggca gcggtccggt gcaggcttcc gagaatgccc
      601 gctcgaaacc ctccattgat ctggaattgc ccgatgagcc gcctctggag atggaagagg
      661 cggccagtgc ctccgatagt tccgccggta gtggctccaa ctttacggtg cccggcacag
      721 acttgttagc cggtactaca ggcagtgccg atttcagcaa gagaccggtc ctgaaaggca
      781 gcatctccgg tggtaacaac gccggtgccc catcccctct gctttccggc agtttgcaaa
      841 tggtgccgaa aggcaccacc gtcgatctga ctttgcaatg ccatttgaat tccgaacttt
      901 cccagaaagg gcaggaagta tttgcccgca tttctcgaga tgtgaccgcc gaagatggca
      961 gcaaggtggt tttgccgggc aagtgggtgg cccatggcta tgttacccga gtggataagc
     1021 agaagcataa cggtcgagac ggcttcgtgg aagtgaagtt cgatcgcatc atcagccccg
     1081 acggtgaatg ggaagtgccg ttcgagacca gtttttccac caaagataat gaactaaagt
     1141 cggccgccaa ggttttattc cgtgatacta aatttgccac catcggcgcg ctggccggtt
     1201 cgatcctgtc tgtgcagatg acaggtttac ctgtggcaat cagcacatat ggaatttccg
     1261 ttggtattgg tggtggtgtc ggagctactt atggtctggt ttgcgccatg gctcgcaagg
     1321 gcaaaatctg ttccgtctac ccgggcgacc acgtgcagct taaactttcc gaacccttga
     1381 gtttgcccgg ttttaatcct ctcgctctcg attcggccaa aaacagctct catctgccgg
     1441 gcttttccct ggccgtcact gatttcaaat ttgagaaaga ccccgtttct caagataaga
     1501 ggggtcaggt gctgaggctc tggcttacgg ccgaaaatcg cagccggcgc agtctcaatt
     1561 tgagaaatct gcgtgtggtt tcggagatgg atgatatata cgctccccat ttgtcggcag
     1621 ccatgatgcg cttccaggaa ctggctccgg gggggcggca atctctggtt ttgccctttt
     1681 ccgtggactc taagaagcac aaatactccc tggtgctcct caaggatgct tccggcgccg
     1741 agctggccag agcccctata aattgagcgc cccattcggg atctatatgt aacctggtaa
     1801 taattctcat ttttctctta aattttgcgg gtttccccac tgagattatt tgcagaaaga
     1861 ctaaatgcag ctaatatgga cctgtactta agacttgatc atgttgagag agataaagcg
     1921 ctcaaagaca cctgttggat ataggagcaa tatggttgtt atattcgaga tgcttccttc
     1981 tttcttgcac ttgatttagt tgaaaaatcc cgggagctgt taatcccgct aggggggacg
     2041 atgaaggcgc gaatccagga aat
//
LOCUS       DAOQNZ010000775         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35737_length_2062_cov_3.682113, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000775 DAOQNZ010000000
VERSION     DAOQNZ010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35737_length_2062_cov_3.682113"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKW73_13220"
     CDS             <1..830
                     /locus_tag="PKW73_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74298.1"
                     /translation="GPGLFAVATRYHLKLYDLPKAITASSYVYPYEHLEEVAQWVSGV
                     AGTLPPSVELSLFMVNAPADLADKCKESGGKVCMVTATMFADSAEEAAQTLSRLDSCP
                     LLPYVLKSSTCEPMTFEQLFDASGALWPSGLRCNVDAMFSDENLATVVASVKEHYKTH
                     ESPSTVVMFAVFTGKNVPEPLDPTMAFSMTARLYGGPWTMWKEASADERNIAWHKKCV
                     ELLSPHIKGHYVAESDTVANPEFSRTSYSQKNWQRLSELRAKYDPDGVFFDYNQGLT"
     gene            complement(956..1798)
                     /locus_tag="PKW73_13225"
     CDS             complement(956..1798)
                     /locus_tag="PKW73_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74299.1"
                     /translation="MPLPELRQSTNSNLSHPQRVFTLALTTGVNFAAYALFHKLACLI
                     TASFSELPLLLVLLAFGMGCCTPFLLGVLLCSWQRKREQAFEILPRPCQPEKDNLLLA
                     EKILYSGRLGIFTWSAICLGLIIGFQITHLSSLAIVLSVFAVNLLAQHLITKKLARLA
                     MQMEASADAVPVPPLVSTPVPTLVPTLPEEKPSGQQAQPGFREKQLNDWLGSLKCTDS
                     VLEAHSLAYKCLCHLKPKQPPAMEEGNIHTLVEELMRRKQHLEADSISANYLRILEED
                     TSRS"
     gene            complement(1836..>2062)
                     /locus_tag="PKW73_13230"
     CDS             complement(1836..>2062)
                     /locus_tag="PKW73_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74300.1"
                     /translation="HHNPGNENAGCHRSAHSSQKERSLIADTVKEARRFAPQVGLCYA
                     ELKGLPSVKTTSPCLEQVPVNIRALQRVHW"
BASE COUNT          441 a          531 c          564 g          526 t
ORIGIN      
        1 ccggtcctgg tctctttgcc gttgccactc gctatcatct caagctttat gatcttccca
       61 aagccattac cgcatccagt tatgtttatc cttatgagca tctggaagaa gtagctcagt
      121 gggtttccgg tgtggccggt acattgccgc cctccgttga attgagtttg tttatggtaa
      181 acgcgccggc ggatctggcc gataagtgca aagagagcgg cggtaaagtc tgtatggtga
      241 ccgccaccat gtttgccgat agtgccgaag aagccgcgca gaccctctcg cgtctggata
      301 gttgtccact tttaccctat gttttgaaga gttccacttg tgagcccatg acattcgagc
      361 aactcttcga tgcctccggc gctctctggc cgtccggttt gcgttgcaac gtcgatgcta
      421 tgttctccga tgaaaacttg gctacggttg tggcttccgt caaagagcac tacaaaacac
      481 atgaatcgcc ttcgacggtg gttatgtttg ccgtcttcac cggcaagaat gtgcctgaac
      541 ctcttgaccc caccatggct ttctcaatga ccgccaggct ttacggcgga ccctggacca
      601 tgtggaaaga ggcttcggca gatgaacgta atatagcctg gcacaaaaag tgtgtggaac
      661 ttctttctcc acacatcaaa gggcactatg tggctgaatc ggataccgtt gccaatccgg
      721 aattttccag gacttcatac tctcagaaga attggcagcg gctctcggaa cttcgagcca
      781 agtacgatcc cgatggagtc ttctttgatt acaaccaggg cctcacttaa gccttcctct
      841 ctttgcagaa gggggcgaag gcgaaattat tgcgaaaatc gctcagtgtt tatttttccc
      901 cttgagaagc ctggcgccgg aagttcgcac ttcttctaac ccatcgagca gatggctagc
      961 tcctgctcgt atcctcttcc agaattctca ggtagttcgc ggagatgcta tctgcctcaa
     1021 ggtgctgctt tctgcgcatt agttcttcca caagggtgtg tatattgccc tcttccatgg
     1081 ccggcggttg cttcggcttc aggtggcaga ggcatttata ggcgagggaa tgggcttcca
     1141 gaaccgagtc cgtgcatttg agcgagccca gccaatcgtt cagttgcttc tctctaaagc
     1201 cgggctgcgc ctgttgcccg gaaggcttct cctctggaag cgtcggcacc agcgtcggca
     1261 ccggcgtcga caccagcggc ggcaccggca ccgcatctgc acttgcttcc atttgcatgg
     1321 ccagcctggc tagttttttt gttatcagat gctgggctaa gaggttgacg gcaaagaccg
     1381 acaggacaat ggccaggctg gaaagatggg tgatctggaa gccgatgatc agccccaggc
     1441 aaatggctga ccaggtgaag attcccaacc tgccactgta taagattttc tctgccaaaa
     1501 gaaggttatc tttttcgggt tggcagggtc tgggcagaat ttcgaaggct tgctcacgtt
     1561 ttcgctgcca actgcataga agaaccccga gaagaaaggg cgtgcagcag cccatgccga
     1621 aggccagaag gaccaggagc agcggcaact cgctgaaact ggctgtgatc agacaggcca
     1681 gtttgtggaa aagtgcatag gcggcgaagt ttactcctgt ggtcagggcc agtgtaaata
     1741 ctcgttgcgg gtgcgataag ttggagttgg tgctctgcct tagttctggt agaggcaaga
     1801 tagcattact gtcctgcatg ttctaagacc tgtggttacc agtggaccct ctgtaaggcg
     1861 cggatattga ccggcacctg ctccaggcag ggactggttg ttttaactga aggtaagcct
     1921 ttcaattcag cataacaaag tccgacttgc ggggcaaatc ggcgcgcttc cttaacggtg
     1981 tcggcgatca acgaccgttc tttctggctt gaatgagctg agcgatggca gccggcattt
     2041 tcgttacctg ggttatggtg gg
//
LOCUS       DAOQNZ010000776         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_35814_length_2060_cov_3.282793, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000776 DAOQNZ010000000
VERSION     DAOQNZ010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35814_length_2060_cov_3.282793"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..271)
                     /locus_tag="PKW73_13235"
     CDS             complement(83..271)
                     /locus_tag="PKW73_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74301.1"
                     /translation="MTGVGGDLTMKKAYVNIRIGGMYRDNFPILIGGSGACALGQDLM
                     DGWRYKVDRENKLLRFFH"
     gene            complement(314..1285)
                     /locus_tag="PKW73_13240"
     CDS             complement(314..1285)
                     /locus_tag="PKW73_13240"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNA74302.1"
                     /translation="MTFLFGGAARGADDAYKLGLEAYGKGNYAAAASHFDSALQKTQN
                     NPLLWYYDALCYHQLKNWSMAKSRYKTTALSFPLTEPGRRAAAALRAIDPTFVAPNSP
                     ATTSTSGQSDKGSSGASATAENTAPAPKSSTAEADQALAQELTRLPDTAHFYFKKGHQ
                     GHMEVDLMVNGHPVKAIFDTGASALFYKDQLKEAGLDLNKAKVGKGTRGWAGVEVPTE
                     VMPAEIKLGTLTRTINIKMPETSTALSDNLIGQDFIKGYQYEIDDRGGRVDLKKNLGT
                     KQAKLNPLYDIPIQIIGGKEFIVFTVNSQYAKAFIDTGAANTIFDEF"
     gene            complement(1361..>2060)
                     /locus_tag="PKW73_13245"
     CDS             complement(1361..>2060)
                     /locus_tag="PKW73_13245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74303.1"
                     /translation="EALNQIIATNPKAYELYEKRADYLVENGRGKEAISDYTKVIENC
                     TPADKLPLSRALAARGSLELQDNCLEEALRDFSRAIDINPCTYESASYLAKRAEVYER
                     LNKQEEALKDLQKSIDTNKAYPGYLRRAEYYQRQGKDMEALNDLELELTKCSQYLRTR
                     VLHTRADIHYRKKEYEKSLAACNQALKEDNNAVDVLCMRAQVYQAMNKPELAALDRQK
                     VKTLDPDHELPPLR"
BASE COUNT          403 a          491 c          559 g          607 t
ORIGIN      
        1 cctccataag accagagcag gtcatagatt tcggtctccg gcaaagagcg tatttgtttg
       61 ccggagacta gcttagatag agctagtgaa agaaccttag tagtttgttt tcgcggtcta
      121 ctttgtatct ccagccgtcc atcagatctt gcccgagggc gcaagcgccg ctgccgccga
      181 ttagtatggg aaagttgtct cgatacatgc cgccgatacg gatatttaca taggcctttt
      241 tcatggtcag gtcgccgcct actccagtca tgtggatgac ttcgccagtg ggaactgctc
      301 cgaaacgggc cgcttaaaat tcatcgaaga tggtgttggc tgcgccagta tcaataaatg
      361 cttttgcata ttggctgttg acggtgaaaa cgatgaattc tttaccgcca atgatctgta
      421 tcggaatgtc atagagagga tttagcttgg cctgtttggt tcccaggttt ttcttcaaat
      481 ctacccgccc gcctctgtcg tcaatttcat actggtagcc ctttatgaaa tcctggccga
      541 tcaggttgtc acttaaggcg gtgctcgttt ccggcatttt gatattgatg gtgcgggtga
      601 gagtgcccag ctttatctca gcgggcatca cttcggtggg gacttctact cccgcccatc
      661 cacgggtacc tttgccgacc ttagctttgt tgaggtccag tccggcttcc tttagttgat
      721 ctttgtagaa aagtgcactg gcgccagtgt caaagatggc tttgaccgga tgtccgttta
      781 ccatgaggtc gacttccatg tgaccctgat gacctttctt gaagtagaaa tgcgccgtgt
      841 ccggtagtct tgttaactct tgcgctagtg cctggtctgc ctctgcagtt gatgatttgg
      901 gggccggagc tgtattctca gcggttgcag aggcgccgct ggagcctttg tcgctttgcc
      961 ctgaagtaga tgtggtggca ggagaattgg gcgctacaaa ggttgggtcg atagctctaa
     1021 gagcggcggc ggctcttctt cctggctcgg tcagagggaa agataaggca gtggttttgt
     1081 agcgactctt tgccatggac caatttttca attggtgata acaaagagca tcgtaatacc
     1141 agagtagagg attgttttga gttttttgca gggccgaatc aaaatggctg gcggctgcgg
     1201 cataattgcc tttgccatag gcctccagcc ctagtttgta agcatcgtca gctccccggg
     1261 cggcgccgcc gaacaaaaac gtcataacta agatggctgc tttcagaccc ctgttcgtta
     1321 tgtcttccgt cgatcgcggc accgtatttt tcatctcttc ttaacgcagc ggcggtaatt
     1381 cgtggtcagg atcgagtgtt ttgactttct gacgatctag tgcggcaagt tccggcttat
     1441 tcatggcttg atagacttga gcacgcatgc agagcacgtc tacggcattg ttgtcttctt
     1501 taagggcttg attgcacgcg gctagcgact tttcgtattc tttcttccgg taatgtatgt
     1561 cggcacgtgt atgcaggacg cgggtgcgca ggtactgact gcacttggtc agttccagtt
     1621 cgagatcatt gagggcttcc atgtctttgc cctgccgttg atagtattcg gcccggcgta
     1681 aatagccggg gtaagcctta ttcgtatcta tggacttttg caagtccttg agagcttctt
     1741 cttgtttatt gaggcgctcg taaacttctg ctcttttagc caggtaggag gcgctttcgt
     1801 aggtacaggg gttgatatct atagcccggc tgaagtctct caaggcctcc tccaggcaat
     1861 tgtcttgcaa ttccaaactg ccgcgtgccg ccagagctcg gctcaagggc agcttgtcgg
     1921 cgggagtgca gttttcaatc accttggtgt agtcggagat ggcttccttg cctctcccgt
     1981 tttcaaccag ataatcggct cgcttttcat agagttcgta ggctttggga ttggtggcaa
     2041 tgatctgatt gagagcttcc
//
LOCUS       DAOQNZ010000777         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36082_length_2052_cov_4.055583, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000777 DAOQNZ010000000
VERSION     DAOQNZ010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36082_length_2052_cov_4.055583"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PKW73_13250"
     CDS             complement(<1..1139)
                     /locus_tag="PKW73_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA74304.1"
                     /translation="MGKQILLVDSDQQFAKLVASVLEGRGHKIVQADSLKAAEAALNK
                     PETPFDLMIVGSPMPQGDELHWILERRRSGMITKLVLVGESGKHIESLRTKITADHAV
                     ALSVHKPLIPYIFGAQIDRTIDDDRSEAAAQKMKDFETMFLALVTKYARVLPNRINEL
                     AEAIERAKNNPASADLSAEVRGLAHKIKGTGGSLGFRRVSELMAYIEDAAATVHEKPK
                     EEQLIIWAEVDRKLLEVKEAGDSECQEINEAVAASGLKPQQATHNPAMTRILVVDDDR
                     GFLDIVSELGKQRLVEIVRASGLKEALEQAVVYPLDAALINVLPDHPEQCFGLARQLR
                     ELPGYENLPLAFVSGQALVKNMVEAAHAGASLYLDKPLASDALEKA"
     gene            complement(1168..>2052)
                     /locus_tag="PKW73_13255"
     CDS             complement(1168..>2052)
                     /locus_tag="PKW73_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74305.1"
                     /translation="NQPAPAPVQPQPPVPAPMPVQTPVPGPMPQQMPQQMPQQMQYPP
                     QPMPGQQPMGYPPQQMQYPPQQMQYPAQPYPQTSMPEPKIPLDYHLIDKRANILLGTL
                     LKEAGLISEPTLEAALKLQELVRDEKLTTEKAPDVLKRLHAMGGSIDQYLTKGDLEKP
                     AGLQAGSRQAVAGHEQAQARPQAPAQGGQRDLKGAFDLLQKAQLLTESDLTTAVNVRK
                     KHGGDVVQILEAAGKVHQKTVEAALTSLPLIREGLMKIEQVIMALNYCERMRVGFDEA
                     LDEMGWQNPRKLRTDLPL"
BASE COUNT          368 a          563 c          571 g          550 t
ORIGIN      
        1 gccttttcca gggcatcgga agctagcggt ttatctaagt aaagtgaggc gccggcatgg
       61 gctgcctcta ccatgttttt caccagcgcc tgtcctgata cgaaggccag tggcaagttc
      121 tcgtatcctg gtagttctct cagttgcctg gccagtccaa aacattgttc cggatgatcc
      181 ggcaagacgt taatcaaggc tgcatcaagg gggtagacca cggcttgttc cagggcttct
      241 ttcagacctg aggcccgcac gatttccacc agtcgttgct tgcctagttc cgagacgata
      301 tctagaaaac cgcggtcatc gtctacaact agaatgcggg tcatagccgg attgtgggtt
      361 gcttgttgcg gcttcaagcc cgatgcggca actgcctcgt ttatttcctg gcattcggaa
      421 tcgcctgctt cctttacttc cagcagtttt cgatctactt cagcccagat gattagctgt
      481 tcttctttcg gcttctcatg gacagtggct gctgcatctt cgatgtaagc catcaattcc
      541 gacacccgcc gaaatcccag agagccgccc gtgcccttta tcttgtgggc cagaccccgc
      601 acttccgccg agagatcggc gctggccgga ttattcttgg cccgttcaat ggcctccgcc
      661 agttcgttga tgcgattggg caagaccctg gcatatttgg tgacgagagc caggaacatg
      721 gtctcgaagt ctttcatctt ttgagcggct gcttccgagc gatcatcgtc gattgtgcgg
      781 tctatttgcg ctccaaaaat gtaagggatg agaggcttat gtactgaaag cgccactgca
      841 tggtcggccg taattttagt gcgcagtgat tcgatatgct tgccgctttc gcccaccagc
      901 accagtttgg taatcatgcc gctgcggcgg cgctccaaaa tccagtgcag ttcatcaccc
      961 tggggcatgg ggctgcctac gatcatgaga tcgaaaggtg tttccggttt attcagggct
     1021 gcttctgctg ctttcaggga gtccgcctgg acaattttat gaccgcgccc ctccagcacg
     1081 gatgcgacca gcttggcgaa ttgctggtcg ctgtctacca ataaaatttg ctttcccatg
     1141 cccgcctatc ctgctttgtt gcgcccctta caatggcaag tcggtgcgca gtttgcgggg
     1201 gttctgccaa cccatttcat cgagagcctc atcgaagccg actctcattc gctcgcaata
     1261 gttgagagcc atgattacct gttcgatttt catgagccct tctcggatca ggggcagact
     1321 ggtgagtgct gcttccactg tcttctggtg tacttttccg gctgcttcca aaatttgcac
     1381 tacatcgcca ccgtgctttt tgcggacgtt gacggctgtg gttagatcgc tttcggtcaa
     1441 aagttgcgct ttttgcaaca ggtcgaaagc ccctttcaaa tctcgttgac cgccctgggc
     1501 cggcgcttgt ggtctggcct gggcttgttc atgaccggct acggcttgtc tggaacctgc
     1561 ctgcaaacct gccggctttt ccagatcgcc ttttgtgaga tattgatcga tgctgccgcc
     1621 catggcatgg aggcgcttca atacatccgg tgccttttcc gtggtcagct tctcgtcccg
     1681 caccaattcc tgcagtttga gagcggcttc cagtgtgggt tccgagataa gacctgcttc
     1741 cttgagaagc gtgcccagca gtatattggc ccgcttgtcg atgagatgat aatcgagcgg
     1801 aattttgggc tccggcatgg aggtttgcgg ataaggctgg gccggatact gcatctgctg
     1861 gggcggatac tgcatctgct gcggcggata acccatgggc tgctgaccgg gcatgggttg
     1921 aggcggatac tgcatctgct gaggcatttg ttgcggcatc tgctgcggca taggaccggg
     1981 taccggagtt tgcaccggca tcggtgccgg caccggtggc tgcggctgca cgggagccgg
     2041 tgccggttga tt
//
LOCUS       DAOQNZ010000778         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36088_length_2052_cov_3.532799, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000778 DAOQNZ010000000
VERSION     DAOQNZ010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36088_length_2052_cov_3.532799"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKW73_13260"
     CDS             complement(<1..244)
                     /locus_tag="PKW73_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74306.1"
                     /translation="MSSPPDSIQEERLRLNGRPVHFPTRLAGDGTTTAGGSDGAMADQ
                     AETLLLAGNWHTAREPLAEAEADEKLPVLLLHSFGGN"
     gene            complement(326..952)
                     /locus_tag="PKW73_13265"
     CDS             complement(326..952)
                     /locus_tag="PKW73_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74307.1"
                     /translation="MLTKEGNFAASLQGKRHNWNELEPSVYQKKSPYPADISLSHGQW
                     CDHCHQHFLRIENNKDRTGEWLDATGAWLKLIGEVILEGTIALVKNLVIGLVTLFVMI
                     VWLIESFCTGAQEDARRYEADLSEKDGLNSSTRDALNRIHQALLLERQGLLKGIGQVE
                     NTLYFHVGHLPVLRKQEEERLAAFRDALHQTEQQLDDVEAMLKTHKHA"
     gene            complement(1098..1655)
                     /locus_tag="PKW73_13270"
     CDS             complement(1098..1655)
                     /locus_tag="PKW73_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNA74308.1"
                     /translation="MTRDKIEGMQTQSQNGENPLQTQYAELMAPPDRKFESLNSTGSG
                     TNNLPDLTIPGSGIDISKQADTPFRFIEAQFPENRGKIPEKSQYTLAKEEAAQRMRDG
                     TDVFHRIKEAGKIEKPSEHVVKKGETYWSVAHQLTSKNVERPTAGDVKFMVKKLAAYN
                     GKTEVEAANIKPGDIIKIPPDLKRF"
BASE COUNT          386 a          547 c          552 g          567 t
ORIGIN      
        1 tgttgccgcc gaagctgtgc aaaagcagta ccggcagctt ctcatctgcc tccgcttccg
       61 ccaaaggctc cctggctgtg tgccagttgc cggcaagcag cagggtctct gcctgatccg
      121 ccatagcccc gtcgctgccg ccggcagttg tggttccgtc tccggccagg cgggtgggaa
      181 aatgcaccgg gcggccgttc aggcggagcc tttcttcttg aatgctgtct ggtgggctgg
      241 acatgccggt atgataccct gttgcgttgc ctggtgcgct ggcctcaacc gagctgggtg
      301 ctcgcccgcg aatgtcgaac tttcctcagg cgtgcttgtg ggtcttgagc atggcttcca
      361 cgtcgtctaa ttgctgttcg gtctgatgca gggcgtcccg gaaggctgcc agtctttctt
      421 cctcctgctt tctgagtact ggcagatgac cgacgtgaaa gtagagtgtg ttctccacct
      481 ggccaatacc cttcagaagc ccctggcgtt caagcaagag agcctggtgt attctgttca
      541 gagcgtctct tgtgctgctg ttaagaccat ctttctcgga caggtccgct tcatagcgcc
      601 gggcgtcttc ctgggcaccg gtgcagaagc tctcgattag ccacactatc ataacgaaga
      661 gcgtaaccaa tccgatcacc aggtttttga caagggctat ggttccttcc aggataacct
      721 cgcctataag cttgagccag gctccggtag cgtcaagcca ttcaccggtg cggtctttat
      781 tgttttcgat tctcaagaag tgttgatggc agtggtcgca ccattgcccg tggcttaagg
      841 agatgtctgc cgggtaaggg ctcttctttt gatagacact tggttccagt tcgttccagt
      901 tatgacgttt accctgcaag gaagcagcga aatttccttc ttttgtaagc atggctgtgc
      961 tctcccggct tgctttgaga gccaggataa aggcgcggtt cgcctaataa aatgtgttat
     1021 cgcacagagt aaatctagtt gtgggatagc aacttaaatc cagtcggaac ccttctgaat
     1081 actgcttgcc gaagcaatca gaagcgtttc agatccgggg gaatcttgat tatgtcccca
     1141 ggcttgatat tggctgcctc tacctcggtt tttccattgt aggcggccag tttcttgacc
     1201 atgaatttta catcgccggc agtaggcctc tccacgtttt tggaggtgag ctggtgggcg
     1261 accgaccagt aagtctcacc cttcttcacc acatgctcgc ttggcttttc aattttgccg
     1321 gcttctttga tgcggtgaaa gacatcggtg ccgtctctca tgcgttgagc cgcttcttcc
     1381 ttagcgaggg tgtattggga tttctccggt atcttgcccc ggttctcagg aaactgcgct
     1441 tctataaagc ggaagggcgt gtccgcttgc ttgcttatgt cgatgccgga gccggggatg
     1501 gtcaggtcag gtagattatt tgtgccgctg ccagtgctgt tgaggctctc gaactttctg
     1561 tcgggcggag ccataagttc ggcatattgc gtttgcaatg ggttttcacc attttgactt
     1621 tgagtttgca tcccttcgat tttgtcccgt gtcataaaca gcctgctctc cccaggagcc
     1681 cagtaaaaaa agtgccgcct tcatttactt acccactccg gctgaaaacc ctcaccttat
     1741 ggggcaaagc aagagatact ttttgcagga atttaacgca agtcaagggc tttccagcac
     1801 atagcttttg tcttaagtga agggtgaggc ggcatcccga ttgttgagcc cggttcgtcc
     1861 gctcaccgca agtttctcgt caattatgat atgacttccc gaccggcaat ctcaggctat
     1921 tttttgacca tcgaagtaga tgtcttgcca gacgtcgttg taagcataca tctgccaggc
     1981 tcccaccgag cgatcgccgt taaccaggtt gttgaaggcg cgatgattcc tgccgcccag
     2041 tatgccgggg gg
//
LOCUS       DAOQNZ010000779         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36146_length_2050_cov_3.485714, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000779 DAOQNZ010000000
VERSION     DAOQNZ010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36146_length_2050_cov_3.485714"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKW73_13275"
     CDS             complement(<1..674)
                     /locus_tag="PKW73_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74309.1"
                     /translation="MLQTGVRTECENDIYCRHKGKILVKVAPAGTQAEPADTVYLATF
                     VKNLHSVGFRLTDEVVTAAAKLSLPELTSLNNELLAVLAYHKGYFQKLEPLYPNFPSQ
                     VMKMSEAELYFNAALHYLTGGIFKPKTLKLPRPALSEEGSLVEIGLGSREEFESIFFN
                     LVKSVVSFSPVNQAELSWFLTGYERAAALIDSLPADFPNRENKIYLLKLILERDPDFV
                     LKHLTTT"
     gene            990..1277
                     /locus_tag="PKW73_13280"
     CDS             990..1277
                     /locus_tag="PKW73_13280"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNA74310.1"
                     /translation="MKIQNRRVITQFIAENADLAAGLERFVKIVSAAHWNHPGDVKQT
                     FAIADLIGNNKWIFNISGNNYRLLAMVWMHNGIVHILKVMTHADYDKETFS"
     gene            1289..1948
                     /locus_tag="PKW73_13285"
     CDS             1289..1948
                     /locus_tag="PKW73_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74311.1"
                     /translation="MDPSSKKYQALIRRLPLRPLKSEQDLDAALQLSIELNEKFQTLD
                     EGEKDYLEMLTQVIQSYEQKFYQIEDSAKPHEVLKELLEQNNMIQKDLRVLLGVPSGR
                     ASELVRGERELSKEQIMTLCLRFKVEPSLFLPKQKSTDISETYKTEPVPSFQTVCESI
                     ESVIAEARNSNKTYLCDCDGLLMTITRSSKIDELMNAYDKFIQASDKKPERKLSIRYF
                     D"
BASE COUNT          610 a          501 c          490 g          449 t
ORIGIN      
        1 gtggtggtca aatgcttgag tacaaaatca ggatcgcgct caagaataag cttgagcaaa
       61 tagatcttgt tctcgcggtt gggaaaatcg gcaggcaagc tgtcgatcaa tgctgccgcc
      121 cgctcatagc ctgtcaaaaa ccagctcagc tcagcctgat tgacgggcga aaagctgacc
      181 acagacttga ccaggttgaa gaaaatagac tcgaactctt ccctgctacc aagacctatc
      241 tctaccagac tgccctcttc gctgagggca gggcggggca gtttgagagt cttgggctta
      301 aagatgccgc cagttaaata atgcagggcg gcattgaaat aaagctcagc ctcgctcatt
      361 ttcatgacct ggctgggaaa gtttggatag agcggttcca gcttttgaaa gtagccttta
      421 tggtaggcaa gcaccgccag aagttcgtta ttgagggagg tcaattccgg aagcgacagc
      481 ttagcagcgg ccgttaccac ctcatcggtg agtctgaagc ccaccgaatg caagtttttg
      541 acaaaagtag ccagataaac cgtgtcggca ggctcggctt gagtgccggc cggagcgact
      601 ttgaccaata ttttgccttt atggcggcag tagatatcgt tttcacactc tgtgcgaacg
      661 ccggtttgaa gcacagggtc ctctcactga ataaaagatg ccgcggtaag cgatgactct
      721 aaggtttttt gacaaaaata gaaggaagaa ccatcatagc cgcgaccata aatttatagc
      781 atatggcata caaggcccgc ccatctattc acacctgaag cgccgccgaa aggcaagccc
      841 gagttctaat cttatggcaa ccagtggccg ccagtggctg ccggccaccg agtcagacct
      901 ggcaaagatt aaatccgctc ccaaatcggg agatccactt gccgacagtc tcccaatctg
      961 ggagactgtc aaaatgggag cagaaagtga tgaaaattca gaatcgccgg gtcatcacgc
     1021 aattcattgc cgagaacgca gacttagctg ccggtcttga gcgcttcgtg aaaatagttt
     1081 ctgctgctca ttggaatcat ccgggagatg tgaaacaaac tttcgccatt gccgacctga
     1141 ttggcaacaa caagtggatt ttcaatattt ccggaaacaa ctatcgctta ttggcaatgg
     1201 tctggatgca taacggaata gtacacatct tgaaagtcat gacccatgcc gattatgaca
     1261 aagagacatt ctcgtaaagg tgacaaaaat ggaccctagc agcaagaaat accaggcatt
     1321 aatcagacgt ttgccgctac gtccgctaaa gagcgaacaa gatctcgatg ccgccctgca
     1381 attgtccata gaattaaatg aaaaattcca gacactagac gaaggcgaga aagactatct
     1441 tgagatgctc acccaggtta ttcagtctta tgaacagaag ttctaccaga ttgaagatag
     1501 cgcaaagccg catgaggttc tgaaagaact cctggaacaa aacaacatga ttcagaaaga
     1561 tctgagagtg cttctcggcg taccatcagg ccgtgcaagc gaactggtga gaggagaacg
     1621 agaactgtca aaagagcaaa ttatgacctt gtgcctgcgc ttcaaagttg agccaagtct
     1681 cttcctgcca aaacaaaaat ccactgacat ttcagaaaca tataaaaccg aacctgttcc
     1741 cagttttcaa acggtctgcg aatctataga gtccgtcata gcagaggcga gaaactccaa
     1801 caaaacctat ctctgtgact gcgacggctt gctgatgact atcaccagaa gttccaagat
     1861 tgacgaactg atgaacgcct acgataagtt catccaggca agcgacaaaa agcctgaaag
     1921 gaagttgtct atcagatact tcgattgaac cggcccgagg ggaagcactg ccagccaggc
     1981 gagaacccgc cgccacgggc atcattaaca ccagctaagc aaatcaccat tttcagacct
     2041 ttcattagca
//
LOCUS       DAOQNZ010000780         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36173_length_2049_cov_4.290873, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000780 DAOQNZ010000000
VERSION     DAOQNZ010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36173_length_2049_cov_4.290873"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="PKW73_13290"
     CDS             <1..1542
                     /locus_tag="PKW73_13290"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNA74312.1"
                     /translation="GLGVHGAGVATAVCAGNTLSSSVAETVSVAALRGQLYSLADDLH
                     PAAVKLGYIDNLAQVDALVEFIEQHKPAFLVYDPDFSRLEGDSSFRGQTGQAAVEGET
                     AALPPLLRGIKERLLPLCHLLTLDLQDALTLLGLTAEACPGGDDRGLLDAFIEELGLK
                     LVALGPKSVLLKGGDRKGSFSQDYFTNGLTGLWLTSLKRTSQSTLGTGDTLSSAITAG
                     VALGHGILDGLIIAKSYLNQTLDQAPHIGRGNGPTGHGNWTCDQSTLPWLTLSGEEGR
                     HRLSFRPANNMGFYPIVDSFAMVETMVNAGVKHIQLRIKELSGEALSEEIRESARLCR
                     QAGAELFVNDYADLAIKHGAHGVHLGQADLVMQDLSRVAEAGLKLGVTALSYWEVARA
                     LALKPSYITVGPIFFDDAPEREAHQGLSALLRWRKVLDFPLVAAGGIVLANAQSVLAT
                     GVDSIAVYRDLSGQSDLTLRLEKWLRLFERKGMPSGIDRSFDASVKDTGSEPPRIAHD
                     SGLKI"
     gene            1547..>2049
                     /locus_tag="PKW73_13295"
     CDS             1547..>2049
                     /locus_tag="PKW73_13295"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNA74313.1"
                     /translation="MNTYLIGFLAVLATIILSVGGMLLVRKRVSFDSLVSYHEVAGYL
                     LSVVGTLYAVVLGFVVVDTMQHTQDLRVLVDKEASGLANIYLCASGLPEPKRGRLRSL
                     CRQYAEAVVGDEWKSMESGGYSVQAFSTVWALWHEISSIQPVSESEKTLHQQLVSEIC
                     SMTESRRT"
BASE COUNT          392 a          563 c          590 g          504 t
ORIGIN      
        1 gggctcggcg tgcacggtgc gggcgttgct actgccgtct gtgccggtaa taccctctct
       61 tccagcgtgg ctgaaaccgt ctcggttgcc gctctgcgag ggcaactcta cagcctggcc
      121 gatgacttgc atcccgccgc cgtaaagtta ggttacatcg ataatcttgc ccaggtggat
      181 gccctggtgg aattcatcga gcagcacaag ccggcctttc ttgtttacga tcctgatttc
      241 agtcgcctgg aaggcgacag cagttttcgc ggtcagaccg gtcaggctgc cgtcgaaggt
      301 gaaacagccg ctttgccgcc gcttttaaga ggaatcaagg agcgcctgct gcctctgtgc
      361 catttgctca cccttgattt gcaagatgcc ctcaccctat tgggtctcac tgcagaggct
      421 tgtcccggcg gcgatgatcg cggcttgctc gatgccttca tcgaagagct cggtctgaag
      481 ttggtggcac tcggtcccaa gtcggtcttg ctaaaaggcg gagatcgcaa aggttccttc
      541 agtcaggatt actttaccaa tggtctgacc ggactatggt tgaccagtct taaacgcacc
      601 agccagagta ccctcggtac cggcgatacg ctttccagcg ccattactgc cggtgtagcg
      661 cttggtcacg gtatcctcga tggtttgatc atcgccaagt catatttaaa tcagaccctt
      721 gatcaggctc cccatatcgg ccgcggtaac ggtccgaccg gccacggtaa ctggacctgc
      781 gatcagtcca ccctgccctg gttgactctc tccggggagg aaggacgcca tcgtttgtca
      841 ttccgaccag ccaacaatat gggcttctat cccatcgtgg atagtttcgc catggttgag
      901 accatggtta atgccggtgt caaacatatt caactgcgca tcaaggaatt gagcggtgag
      961 gctctcagtg aagagatccg tgagtctgcc aggctctgtc ggcaggccgg agctgagctt
     1021 tttgtcaacg attatgccga ccttgccatc aagcacggtg cgcacggcgt tcatctgggt
     1081 caagcagatc tggtcatgca agatttgagt cgggtggcgg aagccggctt gaagctgggt
     1141 gtgactgccc tctcttactg ggaggtggcg cgagctttag ccctcaagcc ttcctacata
     1201 acagtgggac cgatcttctt tgacgatgct ccggaacgag aagctcatca gggactctcg
     1261 gcgctcttgc gctggcgcaa ggttctggat tttccgctgg tggcggcggg cggcatcgtt
     1321 cttgccaacg ctcaaagcgt gctggctacc ggagtggatt ccatcgcagt ctaccgcgat
     1381 ctgtccgggc agtccgactt gaccttgcgt ctggaaaaat ggttgcgcct ctttgagcgc
     1441 aagggcatgc cttcaggcat agatcgcagc tttgatgcat ccgttaagga caccggttcc
     1501 gagcctcccc gcatcgccca tgactcaggt ttgaagatct agaatcgtga atacctatct
     1561 gatcgggttt ctggccgttc tggctaccat catcttgtct gtcggtggta tgctcctggt
     1621 gcgcaagcga gtcagtttcg actccctcgt ttcttatcac gaagtggcag gctacctgct
     1681 ttccgtggta ggaacgctct acgcagtggt gcttggtttt gtggttgtgg ataccatgca
     1741 acacacccag gacttgcggg tgctggtgga caaggaagcc agcggtctgg ccaatatata
     1801 tttgtgtgct tccggtttgc ctgaaccaaa gcgcggccgt ctgcgcagtc tttgtcggca
     1861 gtatgccgaa gcggtagtgg gcgacgaatg gaagtccatg gagagtggcg gctacagcgt
     1921 ccaggccttt tctacggtct gggctctctg gcatgaaatt tcatccattc agccggtgag
     1981 cgaatcggaa aagaccctcc atcagcagct agtcagtgag atatgcagca tgactgagag
     2041 tcggcgcac
//
LOCUS       DAOQNZ010000781         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36183_length_2049_cov_2.959378, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000781 DAOQNZ010000000
VERSION     DAOQNZ010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36183_length_2049_cov_2.959378"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1641
                     /locus_tag="PKW73_13300"
     CDS             175..1641
                     /locus_tag="PKW73_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNA74314.1"
                     /translation="MSTKKSVQFLPGRHPLDNPAVRTVFQSAGHLPLHLVGGCLRDNI
                     DVPVNSSAPSRNVRPTTDFDFTVGGDALAFAGEMARLLKGHFVVLDESFDIARVVLDD
                     GSYLDFAGYKGSLEEDVRRRDLTINALVMEPGSPDRVIDLVGGLHDLEKNLVRAVSEE
                     SLLEDPLRLLRVYRFAVARAATIEGATRSYLGKAENLARLSQVARERVNYELFTLLNY
                     DGVGKYIHDMGESGLLEALFPELRDCRRVTSNSFHHLALFDHSLETIPQLEAKMPSLP
                     DYIHLSANREVVHGVTRLAVAKLSALLHDIGKPDTWVIQPDGRHSFIAHDKVGAAMVR
                     PLGEREKWPRNISRLVEKLVLWHLRPGQLFHTGEPTQKALNRFYRNAGEDFPELMLIT
                     FADFGATMGPGLSGEKRENLEKSFHRLLNEYPTYMEQVRALPRLLSGEDVMKLLEVGP
                     GPLVGEILQAVNEAQEIKEVTNRAQAKALARLIYDAKK"
     gene            1870..>2049
                     /locus_tag="PKW73_13305"
     CDS             1870..>2049
                     /locus_tag="PKW73_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74315.1"
                     /translation="MNAKVDKLHNYTVIARLDDAIPLNTEEWVATERLLNQVSEFVPM
                     SMLNALTEAIIGICDD"
BASE COUNT          477 a          526 c          560 g          486 t
ORIGIN      
        1 gccggagtgg ataacgccca tgccctcaca tacgccataa tgtggttggg cttgaacacc
       61 ttgagttccc tggttggtgg tctggtttat ttactgggtc atttccagaa acctaaagaa
      121 gagtctgaga tgatggattc agccggctga cagttttgtt tgaaatagcc tcccatgtcc
      181 actaaaaaat cggtgcagtt tctccccggg cgtcaccccc tggataatcc agccgtacgg
      241 acggtctttc agagcgccgg tcacctaccg ttgcatctgg tgggcggttg cctgcgcgac
      301 aatatcgatg tgcctgtcaa ttccagcgct ccttcccgca atgtcaggcc caccaccgat
      361 ttcgacttca cagtcggtgg cgatgcgttg gcttttgccg gcgagatggc acggttactg
      421 aaaggtcatt ttgttgtcct cgatgagagt tttgatatag ccagagtagt tctggatgac
      481 ggctcctatc tagattttgc cggctataaa ggtagtctgg aagaagatgt ccggcggcgc
      541 gatctgacca tcaatgcact cgtcatggag ccaggcagtc ctgaccgggt tattgacctg
      601 gtgggcgggc ttcacgacct ggagaagaat ctggtgcggg ccgtcagtga agagtccctg
      661 ctggaagatc ccctgcgcct tttgcgtgtt tatcgctttg ccgtggccag ggcggctacc
      721 atcgaaggtg ccacccgcag ctacctgggt aaggcggaaa atctggccag gctctcccaa
      781 gttgccaggg agagggtcaa ctacgaactt ttcacgctgc tgaactatga cggtgtgggc
      841 aaatacattc atgacatggg agagagcggt ctactggagg cactcttccc cgagttgcgc
      901 gattgccgga gggtcacttc caacagcttc caccatctgg ctctgtttga tcacagcctg
      961 gagaccatac cgcagttgga agcgaaaatg ccatccctgc ctgactatat ccacctctcg
     1021 gctaatcggg aagtggttca tggcgtcacg cgcctggccg tggccaagtt gtcggccctt
     1081 ttgcacgaca taggcaaacc ggacacctgg gtgattcagc ccgatgggcg tcatagcttt
     1141 attgcccacg ataaagtggg tgcagccatg gtgagaccgt taggtgagcg tgagaaatgg
     1201 cccaggaata tcagtcggct tgtggaaaag ctggtgctct ggcatctcag acccgggcag
     1261 ctctttcata ccggcgagcc cacgcagaaa gccttgaatc gcttctatcg caatgccggt
     1321 gaggacttcc ccgaactgat gctcattact tttgccgact tcggcgccac catggggccg
     1381 ggtttgtccg gcgagaaacg agagaatctg gagaagtcct tccatcgcct gctgaatgaa
     1441 tatccgacat atatggaaca agtcagggct ttgccgcgtt tgctctccgg cgaggatgtc
     1501 atgaaactac tagaggtggg acctggtcct ctggttggcg aaattctaca agccgtcaat
     1561 gaagcgcaag aaattaagga agtgaccaat cgcgctcagg ctaaggctct tgctcggttg
     1621 atctacgatg ccaagaagta agaaagatga aattcttcgc ttgataactg catttcagga
     1681 ttaaggctct cggatggggg cataaaactt tcagcgagat atacgcaagc aacgcataac
     1741 tgcaaaagac ctcgtatatt tccaccccga taaacagaag gcggctgctc aagcagtccc
     1801 ggttgagtat cgggttggta tttcgccgtc aaaaaagtgc tccaacgctt ctatcaacag
     1861 gtgaacaaaa tgaacgctaa agtggacaaa cttcacaact acactgtgat tgccagactc
     1921 gatgatgcca ttccgctcaa caccgaagaa tgggttgcca ccgagagact tctcaatcag
     1981 gtcagtgagt ttgtgccgat gagcatgctc aatgctctta ccgaagcaat catcggcata
     2041 tgtgatgat
//
LOCUS       DAOQNZ010000782         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36210_length_2048_cov_3.708480, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000782 DAOQNZ010000000
VERSION     DAOQNZ010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36210_length_2048_cov_3.708480"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..>2048
                     /locus_tag="PKW73_13310"
     CDS             141..>2048
                     /locus_tag="PKW73_13310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74316.1"
                     /translation="MPDKAEPKSKLPDLKEVERAEPVELREDAPADPVPPSPPSSQSG
                     PDTLSVSQTFNVGAFRTEVEIAAGTIIGGNYKVLRLLGKGGMGQVYLAEQLNLGKNCA
                     IKVIPPNQVTEKAWQRFQTEARAVAKMEHPNLVRVTDLGVHEGTMPFYAMDFVEGQSL
                     DWYLKNNSVMTWQAVLAIFLQVAEGLDYAHKNGIVHRDLKPANIVLVSRPGGLVQAKI
                     LDFGLAKLSQNDASDLRLTATGEIFGSPLYMSPEQCLGESVDHRSDIYSLGCALFECL
                     TGQPPYTGEFGLAVMSKHQLAPVPSLSDVLGKENVPAGLEKVLTKMLAKSPQERYQSA
                     LELSEDFRRVAAGLPVTGGARGGEARGQKWGSAGGSDGKSSVGGKPGADLMGRVSESV
                     RSRPKTSAALALLLCGGIYYICSSGMLNSLSPANTASGASGSGPLAFIDIGPLFPPLV
                     KPTAAELYLRIDETFRKESDAYRDYIDGNVVEKVRRRCREAREDCEARLETMGTEAVS
                     ASIVEIERAGRGAAIAYRMLARLDARVVPYLIKHAGNNDSRQLLMGPVLYAIGQPAAD
                     GLVNRMLQHKEERPTAAALLASMYRKEQRYRFSVDDSLPRQLSPFAINKLLEILRGDK
                     NPQVRSHILAAL"
BASE COUNT          440 a          511 c          627 g          470 t
ORIGIN      
        1 accggttttg ctcatggggt gcgcgctacc ttctgcggtg gtatattcca ttcaggaacg
       61 gcattttaat gatttggagc ccccgtggag ccgtacctgt cttgaagtct aaagccagtt
      121 tcggtacccg ttagaagacg atgccagata aagccgagcc taagtcgaaa ttacctgact
      181 tgaaagaagt tgaaagggct gagcctgtcg agctgcggga agacgcccct gccgacccag
      241 tgccgccatc gccgccttcg tcgcagtcag ggcctgatac tttaagcgtc tcgcagacct
      301 ttaacgtggg tgccttccgc accgaagtgg agatcgccgc cggtactatt attggcggca
      361 attacaaggt cttgaggctt ctaggtaagg gcggtatggg acaggtttac ctggccgaac
      421 agcttaacct gggtaagaac tgcgccatta aggtgattcc tcccaatcaa gttaccgaga
      481 aagcctggca gcgctttcaa accgaggcca gggccgtggc taaaatggag catcccaatc
      541 tggtgcgagt taccgatctg ggagtgcatg agggcacaat gcccttttat gccatggact
      601 ttgtggaagg gcagtcgctg gattggtatc tcaagaataa ttcggtaatg acctggcagg
      661 cggtactggc aatttttctg caagtggccg agggtcttga ttatgctcac aaaaacggca
      721 tagtgcacag ggatttgaag ccggccaata ttgtgcttgt gagccggcct ggtgggcttg
      781 tgcaggcaaa gatactggac ttcggtctgg ccaagctcag ccagaatgac gccagcgact
      841 tgcgccttac agccaccggc gagattttcg gcagcccgct ctatatgagc cccgagcagt
      901 gtctgggtga atctgtggat caccgttcgg atatttattc tctgggctgt gctctttttg
      961 agtgcttaac ggggcagccg ccttataccg gtgaattcgg tctggctgtg atgagcaagc
     1021 accagttggc gccggtgccc agtttgtcag acgttctcgg caaagagaac gtgcctgccg
     1081 gtctggagaa ggtgctgacg aaaatgcttg ctaagtcgcc ccaggagcgc tatcaatcgg
     1141 cgctggagct gagcgaggac tttcgcagag tggcggccgg tctgcctgtc accggcggag
     1201 ccagaggcgg cgaggccagg gggcagaaat gggggagtgc tggaggctcc gatgggaaga
     1261 gtagtgtcgg tggtaagcct ggcgctgatc ttatggggcg ggtatcggaa agtgtgcgca
     1321 gcagaccgaa gacttcggcg gctctagctt tgcttctgtg cggcggcatt tattacatct
     1381 gcagtagcgg catgctgaat tccttgtcgc ctgctaatac cgccagtggt gctagcggca
     1441 gtggtcctct tgcctttatc gatatcggac cgctctttcc tcctctggtc aagccaaccg
     1501 ccgccgaact ttatctgcgc atcgatgaaa cattcaggaa agagtctgat gcgtatcggg
     1561 attacatcga cggcaatgtg gtggaaaagg tgcgccgccg ttgccgggag gcgcgcgaag
     1621 actgcgaggc caggttggag accatgggca cggaagccgt ctcagcttcc attgttgaaa
     1681 ttgaaagggc gggcagaggc gcggctattg cttacagaat gctggctcgt ctggatgccc
     1741 gtgttgttcc ttacctgatc aagcatgccg gcaataacga tagccgccaa ctattgatgg
     1801 gaccggtgtt atatgccatc ggccagccgg ccgctgatgg gctggtcaat cgcatgttgc
     1861 agcacaagga agaaagaccg acggctgccg cactactggc cagtatgtac aggaaagaac
     1921 agcgttatcg gttttcggta gatgacagct taccgcgcca actatcgccg tttgcaatca
     1981 acaagttgct tgagattttg cggggcgata aaaacccgca agttcgcagt catatcttag
     2041 cggcactg
//
LOCUS       DAOQNZ010000783         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36262_length_2047_cov_2.795683, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000783 DAOQNZ010000000
VERSION     DAOQNZ010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36262_length_2047_cov_2.795683"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKW73_13315"
     CDS             <1..237
                     /locus_tag="PKW73_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-quinone oxidoreductase"
                     /protein_id="HNA74317.1"
                     /translation="MAGMRIKAGEGFAAVESPRGILGCYVTSTGGEKPFRFRWRNPSF
                     CNLSVLSELLVDSPIADIMAIFGSIDVILPDVDR"
     gene            311..1684
                     /locus_tag="PKW73_13320"
     CDS             311..1684
                     /locus_tag="PKW73_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNA74318.1"
                     /translation="MISNGNEIGKLTLLSIVWLTIVFVAVWMVTWAVGILLPMILSNS
                     LGEKALLINEILCFLTPVLSILVFIPINAMFMVLIERRALALFTVRKGPNIVGPDGYL
                     QTAADAVKLLFKEDITPTGADAFMFTLAPIVFFAPSIFGAVPLLAAVTNNHGLFQVLN
                     LPTGIFYVLAAASIPVVALVMAGWSSNNKYSLVGGLRSAAQAISYEIPLVLSIITICV
                     LAGTLDLVKITQQQAGGILNWNILGGGALSGIDKFLAGTVTGQPVGNILSTCYPFSGL
                     SYLAVGALIISTIIAFWLYLTGACAEINRIPFDLPEAESELVSGYNTEYSGIKFAIFF
                     LAEFTNLFVVSSIATVMFLGGGDNPIPVWILDSIAPLKQAIALLNDSFLVQTLHLVNP
                     ETLFSTLWVILKVYSLVFFAILIRGTLPRFRIDQLMDFGWKRLIPLSLIVFIVVVFAR
                     GLVICHG"
     gene            1677..>2047
                     /locus_tag="PKW73_13325"
     CDS             1677..>2047
                     /locus_tag="PKW73_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNA74319.1"
                     /translation="MANLLARAFSGLNQVRRGLSTVFGHSFKEPITKEYPQIVPAITT
                     HFRGRLALTVNPETGDHLCISCRQCERVCPDKCIEITSAKSAETGKLELIDFRIDHGL
                     CMFCGLCTEVCPTYCIINTND"
BASE COUNT          401 a          572 c          492 g          582 t
ORIGIN      
        1 atggccggta tgcgtatcaa agccggcgaa ggctttgccg ctgttgagtc gccgcgcggc
       61 attctcggct gctatgtcac ttccaccggt ggtgaaaagc ccttccgttt ccgctggcgc
      121 aatccttctt tctgcaacct ctcggttctt tcggaactgc tggttgacag tcccattgcc
      181 gacatcatgg caatctttgg ttctatcgac gtcatcttgc ctgacgtaga cagataaggc
      241 cgaatcgtcg caagacggaa gttcgagagc aagaagaaaa cctagtagta gtgaaggaaa
      301 tcgagaaata atgatttcaa acggcaacga aatcggaaaa ttgaccctcc tcagcatagt
      361 ctggctgacc atcgtttttg tggcggtgtg gatggttacc tgggcagtgg gaattctttt
      421 gccgatgatt ctctccaaca gcctgggaga aaaggcgcta ctgatcaatg agattctttg
      481 cttcctcact ccggtcctct ccattctggt cttcatcccc atcaacgcca tgttcatggt
      541 tttgattgaa agacgtgccc tggctctctt caccgtccgc aaaggaccga atatcgtagg
      601 accggacggt tatttgcaaa ctgctgccga tgcggtcaaa ttgctcttca aagaagacat
      661 tacgcccacc ggtgccgatg cctttatgtt caccctggcg cccattgttt tctttgctcc
      721 ttccatcttt ggcgccgtgc cgcttctggc tgccgtcacc aacaatcatg gactcttcca
      781 ggttctcaac ctgcccaccg gtatcttcta tgtactggct gccgcttcca ttccggtggt
      841 ggcactggtt atggccggtt ggtcttccaa taacaagtat tctctggtcg gcggcttgag
      901 aagtgctgcc caggctatta gttatgagat cccgctggtg ctctccatca tcactatttg
      961 tgtgctggcc ggcacccttg atcttgtcaa aattacccag cagcaggccg gcggtatcct
     1021 taattggaat atccttggcg gcggcgcgct cagtggtatc gataagtttc tggccggcac
     1081 cgtgaccggg cagcctgtgg gcaacatcct ttccacctgc tatcccttct ccggtctttc
     1141 ttacctggca gtgggcgctc tgatcatttc aaccattatt gccttctggt tgtatttgac
     1201 cggcgcttgc gctgagatca accgcatccc tttcgatttg cccgaagctg aatctgaatt
     1261 ggtttccggc tacaacaccg aatatagcgg catcaaattc gccatattct tcctggctga
     1321 attcaccaac ctctttgtcg tctccagcat tgctacggtc atgttcctgg gtggcggcga
     1381 caaccctata ccggtctgga tccttgacag cattgcaccg ctgaagcagg caattgcttt
     1441 actcaatgat tccttcctgg ttcaaactct gcatttggtc aaccctgaga cgctcttctc
     1501 caccctctgg gttatcctga aagtctattc gctggtattt ttcgcgattc tcatccgcgg
     1561 cactctgccc cgattccgta tcgaccagct catggatttc ggttggaagc gcttgattcc
     1621 gctttcgcta atcgtcttta tcgtcgtggt attcgcaagg gggttggtaa tctgccatgg
     1681 ctaatttatt ggctagagct tttagcggtt tgaaccaggt cagacgcggt ctgtctacgg
     1741 tttttggcca ctccttcaag gagccaatca ccaaggaata tccgcagatc gtgccggcta
     1801 tcaccactca cttccgcggg cgcctggcgc ttacggttaa tcctgagacc ggcgatcact
     1861 tgtgtatttc ctgccgtcag tgcgagcgtg tttgcccgga caagtgtatt gaaatcactt
     1921 ccgccaagag tgctgagacc ggcaaacttg aacttatcga tttccgtatc gaccacggtc
     1981 tttgcatgtt ctgcgggctt tgcacggaag tttgtccgac ctattgcatc atcaacacca
     2041 acgactt
//
LOCUS       DAOQNZ010000784         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36371_length_2044_cov_2.799397, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000784 DAOQNZ010000000
VERSION     DAOQNZ010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36371_length_2044_cov_2.799397"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKW73_13330"
     CDS             complement(<1..753)
                     /locus_tag="PKW73_13330"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNA74320.1"
                     /translation="MPCKGALVTILSLKISEAQRQENGKPMNALTDFLSIVGGATAIS
                     LLGMALVRRKARREALEASHEVGGYLLAIVGTLYAILVGLIVVSAQAKVDTASQMAVT
                     EANMLTNIYHLSRTFDEPCRRQIRQDLYEYATIASSQDWARVEEGEEKEGTVVPYRHL
                     WQDVTAYEPHNDNDKECYARMLDDLEELSDARKYRMVAAKSQLSPVLWSVLIAGGVMI
                     VLFTYFFFVESLLTQTLMTAFVAIFLSMNIYLI"
     gene            974..1447
                     /locus_tag="PKW73_13335"
     CDS             974..1447
                     /locus_tag="PKW73_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74321.1"
                     /translation="MNALTPNIVAPSSDSCRIEAHPWNTFTATRNRMASNCASSPSLL
                     SKLAGRSDYSVQARVAENDSTDSATLERLSLSDSSEVRAAVAQNEHTLRSTVLTLAED
                     LSSDVRYALAENAGTNMALLELLSLDENPYVQGRAIRTKARVLADEAYLTACGRA"
     gene            1450..1650
                     /locus_tag="PKW73_13340"
     CDS             1450..1650
                     /locus_tag="PKW73_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74322.1"
                     /translation="MHPTKTNTLGLTVHVDAFWQQSTKLADGEQFLTAARLRRLRSYI
                     QGKSALYPGSGSCSNQEAEVEL"
     gene            1647..1829
                     /locus_tag="PKW73_13345"
     CDS             1647..1829
                     /locus_tag="PKW73_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74323.1"
                     /translation="MSKGLVLCPIAWVVHCSGCALVKVCPARSILGDFEREPPATDSG
                     GEAEEKSAPPESDADK"
BASE COUNT          482 a          522 c          551 g          489 t
ORIGIN      
        1 aatcaggtag atgttcatgg acaggaagat tgcaacaaaa gcggtcatga gagtttgggt
       61 cagcaggctc tccacgaaga agaagtaggt gaagagcacg atcatgacgc cgccggcgat
      121 aagcaccgac cagagcaccg gggaaagctg gcttttagca gctaccatgc gatatttcct
      181 ggcgtcggac aactcttcca ggtcatccag catgcgtgcg tagcattctt tatcgttatc
      241 gttatggggt tcataagcgg tgacatcttg ccagaggtgt cgatagggaa ctactgttcc
      301 ctccttctct tccccttctt ccacccgggc ccagtcctga gaactggcta tggtggcgta
      361 ttcgtaaagg tcctggcgaa tttgtcggcg gcagggttca tcgaaagttc ttgatagatg
      421 atagatgttg gtgagcatgt tagcctcggt aaccgccatc tggctggctg tgtccacctt
      481 ggcctgggca ctgaccacga tcagtccaac caggatggcg tagagggtgc ccacaatggc
      541 aagcaaataa cctcccactt catgactggc ttccagcgct tcccgtctgg ccttccttct
      601 gactaaggcc atgcctagaa gcgaaattgc cgtagcaccg ccgacgatgg aaaggaagtc
      661 cgtgagagca ttcattggtt taccgttttc ctgtcgttgg gcttcgctga ttttcaggct
      721 caaaatagtg acaagggcgc ccttgcaagg catgggccgc ctgagcaaag tgaaatacta
      781 caggtttgtc ctcattcccg gcaagcctga cggcagaaac acagctatat caataatctt
      841 gacattgtgc cataatgaaa ttgtgtccgg cattattgga tttctccgga cgaagccttt
      901 aaaacttatg aggccggttg agaccggtaa agtctactca aaatcctcgg gtgatctacc
      961 aaagagagga tttatgaacg ctttgacacc aaatatagtt gcgccatctt cagacagctg
     1021 ccggattgag gcccatccct ggaacacttt caccgccaca agaaatcgca tggcatccaa
     1081 ctgtgccagt agtccctctt tgctcagcaa gctggcgggg cggtcggact attctgtgca
     1141 ggctcgggtg gcggaaaacg acagcacaga tagtgctacc cttgaaaggc tttctctaag
     1201 cgattcaagc gaggtaagag cggcggtcgc tcagaacgag cacactctac gctctaccgt
     1261 tcttactctg gctgaagacc tgagcagcga tgttcgttat gccctggctg aaaacgctgg
     1321 tactaacatg gctttattgg aattactctc tctggatgaa aatccatatg tgcaagggcg
     1381 agcgattcgg acaaaggcga gagtgctggc tgacgaagct tatctcaccg cttgcggaag
     1441 ggcttgaata tgcaccctac taaaaccaac acgcttggcc tgaccgtcca tgtcgatgcc
     1501 ttctggcagc aatcgaccaa gcttgccgac ggtgagcagt ttttgactgc ggcaagactt
     1561 cggagattgc ggtcctatat tcagggcaag agcgctcttt atcctggctc tggtagctgt
     1621 tcaaatcagg aagcggaggt tgaattatga gtaagggact ggtcctttgc cctattgcct
     1681 gggtcgtgca ttgcagcggt tgtgcgctgg taaaagtttg cccagcccgg tccatactgg
     1741 gcgactttga gcgggagccg ccagcaacag acagtggcgg tgaagctgaa gagaagagcg
     1801 ctccgccgga aagcgatgcc gacaagtagt ctacatcgcc tagcatggct gactgtccgg
     1861 caatcagtct acatcgccta gcatggctga ctgcccggca agcagtcggc atgtaattgc
     1921 atggctgaac taatccgctc cgtggaagtc cggtagtttt tctaccagta ctttgtctat
     1981 gcggtggcga tccatatcga caatttcgaa agacaatccc tgccacctga acttatctcc
     2041 ggtg
//
LOCUS       DAOQNZ010000785         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36383_length_2043_cov_4.061871, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000785 DAOQNZ010000000
VERSION     DAOQNZ010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36383_length_2043_cov_4.061871"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PKW73_13350"
     CDS             <1..1016
                     /locus_tag="PKW73_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74324.1"
                     /translation="DTRFARLIFTINRGEIPIRCDGFNTFAFIFPGQRVLLPTSEEAA
                     IYKKNFLTESSRAKFDLAHYARPAMPSDSIPSELIMPRSAKGWGEPIAQESEATPAET
                     GHLSHTSLGNSHIELNRTATKYSIKPSIRQNSSIPQFTPVHSQHQAEAAEVNRPQSVP
                     TFEFRPQSAALSGVRTIDMSPPTADTPRIEEPRPPVKPAQYIGSTIQQADDQDLFFSQ
                     GSLELTTLSHYCRVMKFESMSNQDNPGDVLVKLQVFDNQRWQTITTYSIRKEQTQRSS
                     HYADGTVDTVNIDLPTTIARELSFNDFTKNWKNYTKVYFNQKEKSKLEQIVGGTAQLR
                     TAV"
     gene            complement(1050..>2043)
                     /locus_tag="PKW73_13355"
     CDS             complement(1050..>2043)
                     /locus_tag="PKW73_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNA74325.1"
                     /translation="CPAIDEFIEFDKELGFFQQRKVFKGAKPDLFIDLSNSTRGIFLS
                     LLGGCKSFCYQKKPDNGPDQQHAVGNFLETIQPICKDIPDSLFPTIFPDAIAEVLVPQ
                     LLETYKFELRPMIGIVPGVGAQRPHRSWILDGWIYLVRHILSLGTHQPVLIGGADDFE
                     TAQKINEESGDRCLNFCGQLKLDETAAILKCCDIVISGDTGPAHIAVAVGRPVIGLYG
                     PTYPARSGPYGCFNMVLDQSSSCECHGEKSCHLAGPGSPGECMGRIMLPEVIDMLSRT
                     MGNSRSVGSFEEANPPGVFFDEFVLREMQSENAAGAVNEAAEKPTDESEVSSSS"
BASE COUNT          551 a          590 c          477 g          425 t
ORIGIN      
        1 cagacacgag atttgctcgc ctgattttta ctattaacag aggggagatt ccgattcgct
       61 gtgacggttt caacaccttt gccttcattt ttccaggaca aagagtgctc ttacccacaa
      121 gcgaagaagc cgccatatac aagaagaact ttcttacgga aagctcccgc gccaagttcg
      181 atctggcgca ctatgccaga ccggccatgc cttccgacag cattcccagc gaacttatta
      241 tgccgagatc ggccaaaggc tggggtgagc ctatcgccca ggaaagcgaa gccactccgg
      301 cagagacagg ccatctcagc cacacctctc tgggcaactc ccatatcgag ctcaaccgta
      361 ctgccactaa atacagtatt aaaccctcga tccggcagaa ctcctcgatt ccacaattta
      421 caccggtgca tagccagcac caggctgaag cggcagaggt gaaccggccc cagtctgtgc
      481 cgactttcga attcagaccc caatcggcag cgctgagcgg cgtcagaacc atcgacatga
      541 gcccgcccac agccgacacg ccccgcatcg aagagccaag accgccagtc aaaccagctc
      601 aatatatcgg ttccaccatt cagcaggcgg acgaccagga tctcttcttt tcgcagggaa
      661 gcctggagct gactaccttg tcacactatt gccgggtgat gaagttcgaa tctatgtcca
      721 accaggacaa ccccggtgat gtactggtga agctgcaagt ttttgacaat caacgctggc
      781 agactattac cacctactcc atcagaaaag aacagaccca aagaagctcg cactacgccg
      841 acggcaccgt cgacaccgtc aacatcgacc tgccgacgac cattgccaga gagctgtcat
      901 tcaacgactt caccaaaaac tggaagaact acactaaagt ctatttcaat cagaaagaaa
      961 agtcgaaatt ggaacagatc gtgggcggca ccgcacagtt gagaacagcc gtctgagccg
     1021 gaattccttc agggtaagcg tctctccaat taagaggaag aggaaacctc ggactcatca
     1081 gtcggttttt cggccgcttc attgacggca ccggcggcgt tctcagactg catctctctc
     1141 aagacgaatt cgtcgaagaa aacccccggc ggattcgctt cctcaaaaga tccaacactg
     1201 cgcgaattgc ccatggtgcg gctgagcatg tcgataactt ccggcagcat aattctgccc
     1261 atgcattcgc ccggggaccc cggaccggca agatgacagg acttctcgcc atggcactcg
     1321 caagaactgc tctgatccag caccatgtta aaacaaccgt agggcccgct tctggccgga
     1381 taggtcggac cgtaaagacc aattaccggt ctaccgacag ccacggcaat atgggccggt
     1441 ccggtatcac cggatatgac aatgtcgcag cacttgagaa tggcggcagt ctcatcaagt
     1501 tttagctgac cgcaaaaatt caggcagcga tcgcctgact cctcgttgat tttctgagcc
     1561 gtttcaaaat catcggcacc accgattagc accggctgat gcgtccccag agaaagtatg
     1621 tgccgcacaa gataaatcca accatccagg atccagctac gatgaggtct ttgtgcgccg
     1681 actcccggta ctatgccaat cattggtctc aattcgaact tgtatgtctc aagcagttgc
     1741 ggcaccaata cttccgcaat ggcatccgga aaaatggtgg ggaataaaga atccggaata
     1801 tctttgcaaa tcggctggat agtctccagg aaattaccca cggcatgctg ctggtcggga
     1861 ccattgtcag gcttcttctg gtagcaaaat gatttacatc cgcccaaaag cgatagaaaa
     1921 attccccttg ttgaattgga aagatcgata aagaggtcag gcttagcccc cttaaatact
     1981 ttacgctgct ggaaaaagcc cagctctttg tcgaattcga tgaattcatc aatggcagga
     2041 cac
//
LOCUS       DAOQNZ010000786         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36415_length_2042_cov_5.072974, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000786 DAOQNZ010000000
VERSION     DAOQNZ010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36415_length_2042_cov_5.072974"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1187
                     /locus_tag="PKW73_13360"
     CDS             141..1187
                     /locus_tag="PKW73_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA74326.1"
                     /translation="MQTSAQPGALPSLRTENLTKMFGSRVSVDKLNLCIYPGELYALL
                     GDNGAGKTTTIGMLTTLLAPTSGRFFICGHDGIKDGRRIRGLFGVVSQDLSVYNELTV
                     YENLRFLAELYGLKGDTIDKRIEQVIAGSSLVSRLNDRAGNLSGGMQRRLSIACAMIN
                     EPKVLFMDEPTVGLDPAARREIWSSLKDLKKLGVTILLTTHYLEEAEILADRIGIIRG
                     GKLIVEGSVAELSERIQGMRGLSIKLKDTVSADGAEIARKLEKIKERFDILIRHDCLR
                     NTIYLSQPEHVSVNEFLRFVFDWLESEDIPFTKFASGEPNLEDVFLAVARSGPEVENQ
                     SLESSLEKVQGGHH"
     gene            1174..>2042
                     /locus_tag="PKW73_13365"
     CDS             1174..>2042
                     /locus_tag="PKW73_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA74327.1"
                     /translation="MAIISTLYDAVKAIVAKDILAWTRRPLYFLASVLMAVLILVCVG
                     NTISGANDMPFGLYDPGGISELSRHLTESGRFRVRVYDDLEKAKSDLAHEKIVALANV
                     AQDPLEDSVQILTAGNNPLVDDQISMGLLSVLTQKAKELSLPLHSAALYQVHFGLKDY
                     ITAGLTAYLCYVLASMNLGFSWIYEWMEKTYRQIVLAPMGLDAAIIAKTFTVTMEAST
                     VLWLALCLTSPISGFTLGNNFPFLVGSTLLSVFTFTCLALLFACFLRTIRIYTMTVSI
                     LGVALMFVSGIITP"
BASE COUNT          434 a          529 c          550 g          529 t
ORIGIN      
        1 gactcggtga agcgcaccgt gcgttttgtg aatgtgccgc ccacaatcta taaggtgttt
       61 aaaatttccc atatcgaaag tcttatgaaa gctgcaggcg gcgtcagcag caccaaactc
      121 ggaccggctt gctgagcgcc atgcagacaa gcgctcagcc cggggctttg ccctctttgc
      181 gcaccgagaa tctaaccaaa atgtttggta gcagggtctc ggtagataag cttaacctgt
      241 gtatctatcc cggcgaactc tatgcgcttc taggtgataa cggtgccggt aagaccacca
      301 ctatcggtat gcttacgacc ctgctggcgc cgacatccgg gcgttttttt atttgcggtc
      361 atgacggtat caaggacggg cgcagaattc gcggcttgtt cggggtagtg agccaggatc
      421 tttcggtata caacgagctg acggtctatg aaaatctgcg ctttctggcg gaactctacg
      481 gactgaaagg ggacactatc gacaagcgca tcgagcaagt aattgccggg tctagtctgg
      541 tgtcacggct caatgatcgg gccggcaatc tttccggcgg catgcagcgc cgtttgtcta
      601 ttgcctgcgc catgatcaac gaacccaagg tgctctttat ggatgagccg acagtggggc
      661 tcgatcctgc cgccaggcgt gaaatctgga gcagtctcaa ggatttgaag aaactgggcg
      721 tgaccatttt gctcaccacc cactatctgg aagaagcgga aattctcgcc gatcgcatcg
      781 gcataattcg cggcggcaag ctgattgtgg aaggctcggt agcagaatta tccgagcgca
      841 ttcagggcat gcgcggtttg tcgatcaagc taaaggacac ggtaagcgcc gatggagcgg
      901 aaatagcccg caagctcgaa aagatcaaag aacgtttcga tatcctgatc aggcacgact
      961 gtttgcgcaa tactatttat ttgagccagc ccgaacatgt ttcagtaaat gaattcctgc
     1021 gctttgtctt cgactggctg gagagtgagg acattccctt tactaagttt gccagcggtg
     1081 aacccaattt agaggatgtc ttcctggcgg tggcccgcag cggaccggaa gtggaaaacc
     1141 agagccttga gtcgtctctg gaaaaggtac agggtggcca tcattagtac tttgtatgat
     1201 gccgttaagg ccatagtggc caaggatatc ttagcctgga ccaggcgccc gctctatttc
     1261 ctcgccagcg tactgatggc ggttttaatt ctggtttgcg ttggtaatac tatatccggt
     1321 gccaatgata tgcctttcgg gctctacgat ccgggcggta tcagtgaact ttccaggcac
     1381 ctgacggaaa gcggtcgctt ccgtgtgaga gtttatgatg atctggagaa ggctaagtct
     1441 gacctggctc acgagaagat cgtggcactt gccaatgtgg cccaggatcc cctggaagat
     1501 tcagtgcaga ttctcacggc cggcaataat cccctggtgg atgatcagat ttccatgggc
     1561 ttgctttcgg tgctcaccca gaaggctaag gaactcagct tgcctctgca ttcggccgcc
     1621 ctctaccagg tgcattttgg tttgaaggac tacatcaccg ccggattgac ggcctatctg
     1681 tgctatgtgc tggcttccat gaatcttggc ttcagctgga tttatgagtg gatggaaaag
     1741 acctacaggc agattgtgct tgcccccatg ggtctggatg cggccattat cgccaagacc
     1801 tttacggtta ccatggaagc ttccactgtg ctgtggctgg ctctctgtct cacttctccc
     1861 atttcaggat ttactctggg caataacttt cccttcctgg tgggttctac cctgctctcc
     1921 gtcttcacct ttacttgcct ggccctgctc tttgcctgct tcctgcgtac cattcgcatc
     1981 tacaccatga ctgtctctat tctcggtgtg gcgctcatgt ttgtctcggg catcatcact
     2041 cc
//
LOCUS       DAOQNZ010000787         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_36660_length_2035_cov_4.284343, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000787 DAOQNZ010000000
VERSION     DAOQNZ010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36660_length_2035_cov_4.284343"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1118
                     /gene="rfbD"
                     /locus_tag="PKW73_13370"
     CDS             213..1118
                     /gene="rfbD"
                     /locus_tag="PKW73_13370"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNA74328.1"
                     /translation="MAQKQDASNYTSERQMKIVVTGAGGMLGRALTPCLEKRRHEVIA
                     LPKEELDVTNFAEVRSTLQKVKPDLVIHSAAYTKVDQAESEPGLAYFINGYGTENLAV
                     AAGDLDIPVLYVSSDYVFDGEQKQPYTTWDKTRPLSIYGKSKLAGEEAVQRHLNRFYI
                     VRTSWLYGPHGSNFVDTITRMAGERKQLRVVSDQVGTPTCTLTLSEVIADLIVTNRWG
                     VYHATDEGVTNWWEFAREIVKGKDVEVVPIETKDFPRPATRPKYSVLDKTTLISTIGR
                     ELTPWQDALQRYLALNVEGKKALSR"
     gene            1192..1746
                     /locus_tag="PKW73_13375"
     CDS             1192..1746
                     /locus_tag="PKW73_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNA74329.1"
                     /translation="MLKPLTQEAIGHDYNKELSVQDYSKKPVIDGVQLINLNMFVDDG
                     GSLAEIVRFDDNGNLQILPDFKVKQTTFSQMLPGVIKAFHLHYNQEDVWFIMPYDRLL
                     IGLFDARKDSPTYNQTMRFVMGAGRAQALYIPRGVAHGLANVWQNPANMIYFVNQCFD
                     ANEPDERRLPWDILGEDFWQIRKG"
     gene            complement(1818..>2035)
                     /locus_tag="PKW73_13380"
     CDS             complement(1818..>2035)
                     /locus_tag="PKW73_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74330.1"
                     /translation="FLSFVFWPLALALSVIVFAGFPLLIQGLGLAAGAASGAAVAVVF
                     CLAQFVHIVADARIWKLRKPSVRAALS"
BASE COUNT          582 a          568 c          490 g          395 t
ORIGIN      
        1 ctggaaaccc aaacacactt tcgaaaccgg cattcgcgaa accatcaagt ggtacaccga
       61 gaacaccgaa tgggtaaaag ccgtcaccaa accccatgca agcactgcgg gcaaggcaga
      121 gggcactaaa gaactggtta aaacagctca atagtaaacc cttcaggtta gagtaaggga
      181 cagacttaaa tgcacttacg gtggtagata tcgtggctca gaagcaggac gcaagcaatt
      241 acaccagcga gcggcagatg aaaattgttg tcaccggtgc tggtggcatg ctcggaagag
      301 cactgacacc ctgcctggag aaacgccgcc atgaggtaat cgcccttccc aaggaagagc
      361 tagatgtcac aaacttcgca gaggtccggt ccactttgca gaaagtcaag ccggacctgg
      421 tgattcattc ggcggcctac accaaggtcg accaggctga gtcggaacca ggtctggctt
      481 atttcatcaa cggctacggc acggaaaatc tagcggtggc agccggagac cttgatatac
      541 cggtgctcta tgtaagctcc gactatgtct tcgacggcga gcagaagcaa ccttacacta
      601 cctgggacaa aacccgcccc ctttccatct acggtaaatc gaagctagcc ggcgaagaag
      661 ctgttcagag acatttgaac cgcttttaca tagtgcgcac aagctggctc tatggccccc
      721 acggctccaa cttcgtggac accatcaccc gcatggccgg cgaacgcaaa caactgcgag
      781 tagtctcgga ccaggtcggt acaccaacct gcactctcac tctctcggaa gtaatagccg
      841 acctgatcgt caccaaccgc tggggcgtct accacgccac cgatgaaggg gtgacaaact
      901 ggtgggagtt cgcccgggaa attgtgaagg gaaaagacgt ggaagtcgtg cccatcgaga
      961 caaaggactt cccaagacca gccaccagac ccaaatactc cgtgcttgac aaaaccacac
     1021 tcatttccac catcgggcgg gaattgactc cctggcagga tgctctgcaa cgttatctgg
     1081 ccctcaacgt agaaggcaag aaggccttga gccgctagtg ttataattgc agcgtcttcg
     1141 ttaaacacca aaaatcaggc aacaagaatt aacagctaat aatctatgct tatgttgaaa
     1201 ccacttacac aagaagccat cggccacgac tacaacaaag aactctcagt gcaggactat
     1261 tccaaaaagc ctgtcattga tggagtacaa ctgatcaatc tcaacatgtt tgtagacgac
     1321 ggcggctccc tggcagaaat tgtacgtttt gacgacaacg gtaatctgca gatattgcct
     1381 gatttcaaag ttaagcaaac taccttctcg cagatgttgc cgggcgtaat caaagccttc
     1441 cacctgcact acaaccagga agatgtctgg ttcatcatgc cttacgacag acttctgatc
     1501 ggcttgttcg atgcccgcaa agattccccc acctacaatc agaccatgcg tttcgtcatg
     1561 ggagccggtc gcgcccaggc cctctacatt cctcgcggtg tagcccacgg tcttgctaat
     1621 gtctggcaaa atccggccaa catgatttac ttcgtcaatc agtgcttcga tgccaatgag
     1681 cccgacgaaa gaagactgcc ctgggacatc ctcggcgaag acttctggca aattcgcaaa
     1741 ggctaaaagc taagcaataa aatactcgga aaagatgctt cttcagaaag aggcatcttt
     1801 tcttttgcac tctgaaatca ggatagcgcc gccctcacgg acggctttct caacttccag
     1861 atacgcgcat cggcgactat atgcacaaac tgagcaaggc aaaacaccac tgccacagcc
     1921 gctcccgagg cggcaccggc agccaggccc aagccctgta taaggagcgg aaagccggca
     1981 aagactatga cggacaaagc cagggcaaga ggccagaaca cgaaggaaag aaatt
//
LOCUS       DAOQNZ010000788         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37004_length_2026_cov_3.860984, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000788 DAOQNZ010000000
VERSION     DAOQNZ010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37004_length_2026_cov_3.860984"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..1659
                     /locus_tag="PKW73_13385"
     CDS             358..1659
                     /locus_tag="PKW73_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74331.1"
                     /translation="MNEDNTQTDVPSALFAQLHPTLAALETAMGAYQEALKRAWNTYK
                     IFSRAIEEVDPTNWSAKIEAARGSEDELTRIRLLYDEKALTFMSTWLQWQDHKSILMN
                     CRKDLADCIGAALRLHEGAKDNPRDLWLQPFIEARLILARRIHTSSVVEHGEIHAQDA
                     FLKRMQNIQAAAFKVLRTELPNLSRATIYSTALVIPGKGIQPHLNENVDLRAVYGVRL
                     VKEPNYDFPASDRLAALLEECEQVMSQALNEEGAFAHDQNGCINERQHAVRMLDFLHT
                     TDKENAPYSYSCCGTLDQQIAAQNERRLNYEIHRLKLQIICGNARKLLEQVVQTLKEA
                     VAETNQLLMKVEAAEAGTTTTALSISRASIDRNTVERYHTLRMMLNNCLAADVRLRRW
                     AKQQLQIYQTLEQDLSKRAGNRALADAFTEVMKQYAYCLKD"
     gene            complement(1835..>2026)
                     /locus_tag="PKW73_13390"
     CDS             complement(1835..>2026)
                     /locus_tag="PKW73_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74332.1"
                     /translation="PIARVRRSAFIIVGMSIGVMAAPLLIPFGGPMRLLVMVVYMIYV
                     GSGLMQVWSWFSGSKPDGK"
BASE COUNT          539 a          603 c          489 g          395 t
ORIGIN      
        1 tagcagctct ctgcggcagc gccgcgggct tatcagcgac agcagacctt gacgccgctc
       61 accggcgtta gctaagcata aatttgtcct cctgaaccta tctcatccct tctcgccaac
      121 cagacaaaac tctcaaaacc ttcgggtgcg ctcgaactac atggaattcc aggtctgtac
      181 caggtacgga agttcaaccc gccaaaaact tgggtttcag aaacaatgtc gatgaatgag
      241 atacggaaaa caggcaagta cagaacctct ccgcatcaag ccctggggcc gtgccggaga
      301 accaaggtgc gactaccgct caggtattcc cgccgttaac caactcagga cacaagcatg
      361 aatgaagaca atactcaaac agatgtccca tccgcccttt tcgctcagtt gcatccaacc
      421 cttgccgcac tcgagacggc catgggcgcg taccaagaag ccttgaagcg ggcctggaac
      481 acctacaaaa tcttcagtcg cgccatcgaa gaagttgacc cgaccaactg gtctgcaaaa
      541 atcgaagcgg ctcgcggcag tgaagatgaa ttgacgcgca ttcgcctact ctacgacgaa
      601 aaggcgctca ctttcatgag tacctggctg cagtggcaag accacaagag catactcatg
      661 aactgtcgta aagacctggc cgattgcatc ggagcggcac tgaggctgca cgaaggcgct
      721 aaggataacc ccagagacct gtggctgcag ccgttcatcg aagctcgcct catcctggcc
      781 cgccgtattc acacctccag tgtagttgag cacggtgaaa ttcacgccca ggatgccttc
      841 ctcaaacgca tgcaaaacat acaagcggcc gccttcaaag tactcaggac tgagttgccc
      901 aacctcagcc gagccacaat ctacagcacg gcgctggtaa tacccggtaa aggcatccaa
      961 cctcacctaa acgagaacgt cgaccttcgc gcagtctatg gcgttcgcct ggtcaaggaa
     1021 ccaaactacg actttccggc aagcgaccgc ttggccgctc ttctagaaga atgcgaacaa
     1081 gtgatgagcc aggccctgaa tgaagaggga gctttcgcac acgatcaaaa cggctgtatc
     1141 aacgagcgtc aacatgccgt gcgtatgctc gacttcctgc acacgaccga caaagaaaac
     1201 gcgccctatt cctattcttg ttgcggcacc ctggatcaac agatcgccgc ccagaacgag
     1261 cgccgcctca actacgaaat tcatcgcctc aagctgcaga ttatttgcgg caatgccagg
     1321 aaattgcttg aacaggttgt gcaaactttg aaagaagcag ttgccgaaac caaccagttg
     1381 ctcatgaaag tggaagcagc tgaagcaggc accaccacca cggctctctc catcagccgt
     1441 gccagcatcg accgcaatac ggttgagcgc tatcataccc tgcgaatgat gttgaacaat
     1501 tgcctggccg ccgacgtgcg cttgcgccgc tgggccaaac aacagcttca gatctaccag
     1561 acccttgagc aagatctatc caaaagagcc ggcaatagag ctcttgccga tgcttttact
     1621 gaagtcatga aacaatatgc ctattgcctc aaagactaag taactcccag gtgccgtctt
     1681 ctctcacaga aggcggcgcc tcactatttt cttatttaaa tcgccatttg ccactattac
     1741 atatcgtata tctggctgtt ttttttacgc aaggaaaaag agtgggcagg atgaagacat
     1801 ttttcaatgc cttcaccctg cctggagcag ccggctactt gccgtccggc ttgctaccgc
     1861 tgaaccacga ccagacctgc atcaggccgg agccgacgta gatcatgtag acgaccatga
     1921 ccagaagccg catcggtcca ccgaagggga tgagcagcgg cgcagccatg acgccgatgc
     1981 tcatgccgac gatgatgaag gccgagcgac gaacacgggc gatggg
//
LOCUS       DAOQNZ010000789         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37009_length_2026_cov_3.525622, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000789 DAOQNZ010000000
VERSION     DAOQNZ010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37009_length_2026_cov_3.525622"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..1448)
                     /locus_tag="PKW73_13395"
     CDS             complement(342..1448)
                     /locus_tag="PKW73_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74333.1"
                     /translation="MLKTPLPFLSSVQPSDFPTINRSVSHTFCPLMLDELQREVTSAP
                     IVRIMEAMSFHSESEAERHLTGLMIRARYGESLAGSDAERREDDARKLVAEAFLCTNK
                     AESLAKASEALALFPGFAEAYIILSEEEEDLAKRLDLLETAVGIASAQFDSELFAAPE
                     KVFWQRMATRPYMRCRAALALELWQQGKKQAAKEHCKALLKLNPRDNQGLRFHLLNWL
                     LACDLADPCVDEYFSLYEEEGTAFGLYANVLWNFVRHGDQKRSQKALEKALEGNKFVP
                     TLLLGAAPTPQFTSRRVERGSVPEAVAYCRIGGAAWQNIDGALPWLKQKCASMLTSKT
                     LLRKIETAEDWHQIGTWAGQVPMYLNIGRQKVWF"
     gene            1755..>2026
                     /locus_tag="PKW73_13400"
     CDS             1755..>2026
                     /locus_tag="PKW73_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74334.1"
                     /translation="MPKSKPNNVVDFRTGKPLPVATNVPREVIPKAVKPDNTSQTDKP
                     GKASKSKPKADAGVTTQVKSAKAGQEKKPGKQATTAQFAHDWEFKPR"
BASE COUNT          577 a          553 c          454 g          442 t
ORIGIN      
        1 cccgttttcc tcaagcagct ttaaccagca agcacaaagt tatctcgctt tccgttcatg
       61 ggcatttgat gttgagcagt ccataagccg ccaggattac ccaaaccaag ctgtgaaata
      121 tgatctttga acacaatctt gttttagcgt gtcacataaa gccttttaac gcccgatata
      181 caccctaaca gctcacaaac gatacgttaa cacaactcaa aaaccgccgt ctgcaatcac
      241 atgttgttgg actaacaccg cattctgcac aaaaacttag agcccctgcg gtactcaatc
      301 aagctggctt cggtaagctc ggttctaccc ggagcctcaa cctaaaacca aaccttctgg
      361 cgcccaatat tgagatacat aggcacctga ccagcccaag tgccgatttg atgccaatct
      421 tcagccgtct caatttttct caaaagtgtc ttagaagtca acatcgatgc gcacttctgc
      481 ttaagccagg gcagcgcacc atctatgttc tgccaggccg cgccaccgat gcggcaatag
      541 gcgaccgcct ctggaaccga accccgttca actctcctgg aagtaaactg cggggtgggg
      601 gcagcaccta gaagcagcgt cggcacaaac ttgttcccct cgagagcttt ctctagtgcc
      661 ttttgtgacc gcttctggtc gccatgtcga acaaagttcc acaagacatt ggcatagagc
      721 ccaaaggcag ttccttcttc ctcataaagg ctgaagtatt catcgacaca gggatcagca
      781 agatcacagg ctaaaagcca gttgagcaag tggaagcgta gcccctgatt atctctgggg
      841 ttcagcttca gcagtgcctt gcagtgctct ttggcagcct gcttcttacc ctgctgccat
      901 agctccagag ccagagccgc tcggcaacgc atatagggtc ttgtagccat tctctgccag
      961 aaaacctttt ccggcgctgc gaaaagctcg ctatcaaact gagcgctggc aatacctact
     1021 gccgtctcaa gaagatccag gcgtttagca aggtcttcct cttcttcgga aaggatgata
     1081 taggcttctg caaaaccagg aaagagcgcc agggcctcgg aggctttggc caggctctct
     1141 gctttattag tacagagaaa agcctctgct accaattttc tagcatcatc ttctcgccgt
     1201 tcggcatctg acccagctaa agattccccg tatctagccc gtatcatcaa accagttaga
     1261 tgccgctctg cttcgctctc agagtgaaaa ctcattgctt ccataatgcg cacgataggt
     1321 gcggaagtaa cttccctctg caattcatcc aacataagtg ggcagaaggt atggcttaca
     1381 cttctattga tggttggaaa atcagaaggc tgcacggaag aaagaaacgg caacggagtt
     1441 ttgagcatca gcgaacctcc cggcggcacg aatgcaatgg cgctaaaatg caatggagca
     1501 aaaatgcaat agcgccaaaa tgcgatggag ctaaaatgca atggagctaa taggtagagt
     1561 aaactttacc tccacagaac aaacccgaac accgcccaaa cgcatacgcg aaggatatac
     1621 cagagttgaa agccgcgccc tgttctgaaa gacaggtgta gccccgagga ttaactatat
     1681 atagtcccgc atattgaaat gatgcagttc cctggcgagc cgtttagtca agtagatatt
     1741 cacgagagaa accagtgccc aaaagtaagc ccaacaatgt tgtcgatttt cgcaccggta
     1801 aacccttacc tgtagccacg aatgtaccga gggaagtaat acctaaggca gtcaaaccgg
     1861 ataatacaag ccaaaccgac aaacccggca aggcaagcaa aagcaaaccc aaagcggatg
     1921 ccggggtaac tacccaagtt aaatcagcga aagccggtca ggagaagaag cctggtaagc
     1981 aagctaccac agcacagttt gcccacgact gggaattcaa accacg
//
LOCUS       DAOQNZ010000790         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37037_length_2025_cov_3.989340, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000790 DAOQNZ010000000
VERSION     DAOQNZ010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37037_length_2025_cov_3.989340"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PKW73_13405"
     CDS             complement(<1..1035)
                     /locus_tag="PKW73_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA74335.1"
                     /translation="MEFVYKIKDEQGRLKEAIASAESSSVLKARLQARGIDVVEIRES
                     TAAGDMQARWKNLQTNVLQVDLFERVTIKDMVVFSRQFAAMISSGVAMLRTLTIIVDQ
                     CPNRKLKRSLDDIRQSIEAGLSLSDAMSRHPQIFDKLYVSMIKAGETGGILADVLKRL
                     ADFLESKSKLNQKVRAAMVYPLVVLVVSVGVFWGMLTFILPIFEGLFRNIGSELPAYT
                     QFLVSLSQYMRSIWAFLFMIILAVSGWLLKRYYATPPGRLHIDTILYGLPLAGDLISK
                     VAIARFSRTFGTLLRAGVPMLTALDVVKDTADNAVVANSIGKIYTDVRQGGSLSKPMA
                     KSSVFPTMVTQ"
     gene            complement(1083..>2025)
                     /locus_tag="PKW73_13410"
     CDS             complement(1083..>2025)
                     /locus_tag="PKW73_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNA74336.1"
                     /translation="EGLGRFRVNVYRDRGSWAAALRVVLSRMPSMEELGLAPVCKEFL
                     TKPRGLILVTGPTGSGKSTTLASMINAINEKQDKHILTIEDPIEFLHHNKFSIISQRE
                     VGSDTKSFANALRSGLREDPDVILVGEMRDLETIHLALTAAETGHLVFSTIHTSSAPQ
                     SVDRIIDVFPPDQQQQIRIMLAHSLVGVVSQTLLPKTEGEAGDSPVTAGRVMAMEILI
                     NNPAVANLIREAKTSQIYTAMQMGASLGMQTLEASLAKLVREGLVSHKEAILKSSRPE
                     DLEQLIGNQGGRGKTEARSSYSEKSEKGYGSSSFGRF"
BASE COUNT          439 a          512 c          575 g          499 t
ORIGIN      
        1 ctgcgtaacc atggttggaa agacagaaga cttggccatg ggtttagaga ggctgccacc
       61 ctgccggaca tcggtataaa ttttgccgat ggaattagcc accacggcat tgtcggccgt
      121 gtccttaacc acatcaaggg ctgtgagcat gggtaccccg gcccgaagga gggtgccgaa
      181 ggtgcgggaa aagcgcgcta tggctacttt ggaaatgagg tcgccggcca gaggcagccc
      241 atagaggatg gtatctatgt gcaggcgtcc gggaggtgtg gcgtagtagc gcttcaagag
      301 ccagcccgag acagccagga tgatcataaa tagaaaggcc cagatggagc gcatgtactg
      361 ggaaagggat acgagaaact gagtgtaggc cggtagctcc gagccaatat tgcgaaacaa
      421 tccttcaaag ataggcagga taaaggtgag catgccccag aagacgccga cggaaacaac
      481 cagcaccacc agggggtaaa ccatggctgc tctgactttc tgattgagtt tggacttgga
      541 ttccaggaag tctgccaggc gtttgagaac gtcggccagg atgccgccgg tttctccggc
      601 tttgatcatg gaaacgtaga gcttgtcaaa gatctgtgga tgtctactca tggcatccga
      661 gagggaaaga ccagcttcga ttgattgcct gatgtcatcc aggctgcgtt tcagtttgcg
      721 gttcgggcac tggtccacga tgatggtgag ggtgcgcagc atagcaacgc cggagctaat
      781 catggctgca aattgccttg agaaaaccac catgtctttg atagttactc tctcgaagag
      841 atcaacctgt agcacgttgg tttgcaggtt cttccagcgc gcttgcatgt caccggctgc
      901 ggtgctctcg cgaatctcga ctacgtcgat gcctcgagcc tgcagcctgg ctttcagtac
      961 gctcgatgat tcggcgctgg cgattgcctc tttcaagcgt ccctgttcat ccttaatttt
     1021 atagacaaat tccatagcaa caatttacca gagcttgctt gcttgttgtg cgccccggga
     1081 ccttaaaacc tcccgaacga tgagctacca tagccttttt cggatttttc gctataactt
     1141 gatctggctt cggttttgcc tcgaccgcct tgattgccga tcaactgctc caggtcttcc
     1201 ggccggctcg acttgaggat ggcttccttg tggcttacca gtccctctct tacgagtttg
     1261 gccagactgg cttccagtgt ctgcatgccc aatgatgccc ccatttgcat ggccgtgtaa
     1321 atttgcgatg tctttgcttc tcgtatgagg tttgccacag ccggattatt gatcaatatt
     1381 tccatggcca tgactctgcc ggcggtgaca ggagaatcac cggcttcacc ttcagtttta
     1441 ggcaagagag tctggctcac gaccccaacc agagaatggg ccagcatgat gcggatttgt
     1501 tgttgttggt ccggggggaa cacgtcgata atacggtcta ccgattgagg cgctgaactt
     1561 gtgtggatag tgctgaatac caggtgaccg gtttcggcgg ctgtgagagc caggtgaatg
     1621 gtttcaagat cgcgcatctc gccgaccagt atgacgtccg ggtcttccct taacccgctg
     1681 cgcagagcgt tggcgaagct ctttgtatcg ctgccgactt ctctttgact gataatggaa
     1741 aatttgttgt ggtgcaggaa ttctatgggg tcttcaatgg ttaaaatgtg cttgtcctgc
     1801 ttctcattga tggcgttgat catggaagca agggtggtgg acttgccgga accggtggga
     1861 ccggtgacta atatcagtcc ccgcggctta gtgagaaatt ctttgcagac cggtgccaga
     1921 cccagttctt ccatggaggg catacgcgaa agcaccaccc tcagtgctgc ggcccaggag
     1981 cccctgtccc ggtagacgtt gacacggaag cgccccaagc cttct
//
LOCUS       DAOQNZ010000791         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37080_length_2024_cov_3.054342, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000791 DAOQNZ010000000
VERSION     DAOQNZ010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37080_length_2024_cov_3.054342"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PKW73_13415"
     CDS             complement(<1..1242)
                     /locus_tag="PKW73_13415"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNA74337.1"
                     /translation="MTSREPLEKPSLKPAHSWLINLCAAMTLLSGLAGTFQPLTVRLS
                     EHPRLFQMLVPYELYHWSNSLSVLAGYLLIFLSINLFKRKQAAWLLSFLLSLATVALQ
                     LARLGSEHIHFLQRSAFVDNLPAISVLPACLTIALLLLTRSSFTVKSEPSYIEALKKV
                     GISLLAVLAYGCLGFFLLDKRDFGLNFQLFDSLICTVRELTLLGNNFDDHTKSRFAHW
                     FLESLRLYGIVAYAGITYSAFRPVHYRLLTLPRERELAGEILDRYGNCSLDYFKLLPD
                     KSYFFNQSKDGFIAYKTVLNVAIALGDATTAGEKLEALIKEYITFCRNNGWSVAFLQT
                     TPAYLEIYRRCGLKDLKVGEDAVVPLKEFAEKTVNKKTFKSIIKKFDKEGYQLVKHQA
                     PHSESLLDQVEEISKQWLSLPG"
     gene            complement(1298..>2024)
                     /locus_tag="PKW73_13420"
     CDS             complement(1298..>2024)
                     /locus_tag="PKW73_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74338.1"
                     /translation="SLAFKRGNAMSASLPKAKKFCLLLTLSTLLGQTIVPLGALAQSS
                     NVEQAPALTLPNYWGDARVNNAAYGDYRNPYVGSPVYNNGTVNTKSTLPSNFGATNYD
                     NQRTAGGSGSGFNPMGLMMGGAMVGGGALMMGAGRMAALGRGGLGHSMGSSPKAHANA
                     MVKAERRRKEQEEKIQKELAQAHEQNMRKRGLNQPRPEENMARNEAPPPSMVSERGLA
                     SQKFQDNVEGEVMEGDTARALSF"
BASE COUNT          432 a          495 c          592 g          505 t
ORIGIN      
        1 gcccggcaaa gatagccact gtttcgagat ttcttctacc tggtcaagaa ggctctcact
       61 atgtggtgcc tggtgtttaa ctagctgata gccttccttg tcgaatttct tgatgatact
      121 tttgaaggtc ttcttattga cggtcttttc ggcaaattct tttaagggca ccaccgcatc
      181 ttctcccact ttcaggtctt taagaccgca gcggcgatag atttccaaat aggccggggt
      241 ggtttgcaag aaagccaccg accagccgtt gttgcggcag aaggttatgt actcttttat
      301 gagcgcttcc agcttctcac cggcggtggt ggcgtcaccg agggcgatgg cgacattcaa
      361 aacagtctta taggctataa atccatcttt gctttgattg aagaaatacg atttgtccgg
      421 tagaagcttg aaataatcta gtgagcaatt gccgtaacgg tccagtattt ccccggctaa
      481 ttctcgttct ctagggaggg taagcaggcg gtagtgcacc gggcgaaagg cactgtaggt
      541 gataccggcg taagcgacga tgccgtagag tcgtaagctt tcaagaaacc agtgggcgaa
      601 gcggcttttg gtatggtcgt cgaagttatt acccaaaaga gtcagttcgc gcacggtgca
      661 gatgagcgaa tcgaagagtt ggaagtttag accgaagtct cttttgtcta gcagaaagaa
      721 acccaggcaa ccgtaggcta acactgccag gaggcttatg cccaccttct taagggcttc
      781 aatataggaa ggttcgcttt tgactgtaaa actgcttctg gtaaggagca agagggctat
      841 ggtgaggcag gccggcagta cgcttatggc cggtagattg tctacgaagg cgcttctttg
      901 cagaaagtgt atgtgttcgc tgcccaacct ggccagttgt agagccactg tagccagact
      961 gagtaagaag cttagtagcc aggcggcttg tttgcgcttg aagagattta tgctcaggaa
     1021 aatgagcagg tagcccgcga gtacgctgag gctgttgctc cagtgataca actcataggg
     1081 caccagcatt tgaaagaggc gcggatgctc cgagagcctc acggtgagcg gctgaaaagt
     1141 gcctgccaga ccggacaaca aggtcatggc ggcgcaaaga ttgatgagcc aggaatgcgc
     1201 cggcttcagt gatggttttt ccaggggttc ccgggaagtc ataggctgct tggtccggcg
     1261 ggtgacgaaa atgcaaaatg cttgcctata gcttacccta aaaggataga gctctggcag
     1321 tgtcgccttc catcacttcg ccttcaacat tgtcctgaaa cttctgagaa gccaggcccc
     1381 tttctgaaac catggaaggc ggcggggctt cgttgcgggc catattttct tccggtctgg
     1441 gctgattcaa gcctctcttg cgcatattct gctcatgggc ctgggccagt tccttctgaa
     1501 tcttttcttc ctgctcttta cgcctgcgct cggctttgac catggcgttg gcgtgggctt
     1561 ttggtgagga gcccatggaa tgtcccagtc cgccccgtcc gagtgcggcc atgcgtccgg
     1621 ctcccatcat caaggcgccg ccgccgacca tggcgccgcc catcatcaag cccatgggat
     1681 tgaagccgga gccggaaccg ccggctgtgc gctgattgtc gtaattggtg gcgccgaaat
     1741 tgctgggtaa ggtgctcttt gtgttgaccg tgccgttgtt gtagaccggg ctacccacat
     1801 aggggtttct gtagtcgccg taggcggcat tgttgaccct ggcgtcgccc cagtagttgg
     1861 gcagggtgag ggccggtgcc tgttccacgt tggaactctg ggccagggcg cccaggggca
     1921 cgatggtctg tcccaacagt gtggaaagag taagaagcag acagaatttc ttggcttttg
     1981 gcaggctggc ggacatggca ttgccccttt tgaaagctag ggac
//
LOCUS       DAOQNZ010000792         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37156_length_2022_cov_2.790036, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000792 DAOQNZ010000000
VERSION     DAOQNZ010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37156_length_2022_cov_2.790036"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKW73_13425"
     CDS             <1..345
                     /locus_tag="PKW73_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74339.1"
                     /translation="PLDYQIVTARAMSMALFLAHKNLPELIVSPFEMLDGDGMTFLTE
                     LRQDEELQSIPFVFCLNEMPEAQFELAAIKEGAAKVVSNNLTVSEFKNLVEPLIHERL
                     VKKGKRQEFTPE"
     gene            462..1076
                     /locus_tag="PKW73_13430"
     CDS             462..1076
                     /locus_tag="PKW73_13430"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNA74340.1"
                     /translation="MSNTTRKPLALLPQLLAATLTVTAAAQAAPVWNIDSSHSDAGFS
                     IKHLMISNVKGSFTNVTGTVEYDGKDPSSIKVDATIDTTTVNTGDKGRDEHLRGADFF
                     DAEKFPKMTFKSKKVKSLGKGKFALTGDLTIKGITKEVTLTVDGPSKEVKDPWGNTKI
                     AASASTKIQRKDFNITYNKAMDNGGTVIGDDVAITLEIEANKKQ"
     gene            1081..>2022
                     /locus_tag="PKW73_13435"
     CDS             1081..>2022
                     /locus_tag="PKW73_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74341.1"
                     /translation="MTKIRKPKIWKRLCLLAAALSCVSASPAAEQDSLKQAVALFNKG
                     DYAACTALINRDINGSLKNNAAAHYYLGTALLRLNQNNAAAEHYLSTSKLAPGTVYDD
                     YSRKALKSIMARAGNMPNSLKEQYQALTNPGRTGQTAATNSQSTNSQSTNSGYRQTED
                     DDQTLPTAALKDVDSTFISTGRRTADTDRTLVQVMRAIKLVPKNIVDELKAGGISVLV
                     TPTILEAMPGSAMDKPRGYDHGGGYTNCGALFHSPRIFIGERVSYLSSVPYLNRNIAS
                     SMLHEMGHAYDSVKGHLSQSGEFDRCYKEDFSHITNTQ"
BASE COUNT          577 a          594 c          456 g          395 t
ORIGIN      
        1 ccgcttgact accagatcgt cacagccaga gcgatgtcta tggccctctt tctggctcat
       61 aaaaacttgc cggagctgat tgtttcaccc ttcgaaatgt tggacggtga cggcatgacc
      121 ttcttgacag agctgaggca agatgaggaa cttcaatcga ttcctttcgt cttttgcctg
      181 aacgaaatgc cggaggctca atttgagctg gctgccatca aagagggcgc tgccaaggtc
      241 gtgagcaata accttaccgt ttccgaattc aagaacctgg tggaaccgtt aatccacgaa
      301 agattggtca agaagggcaa acgacaggaa ttcacgcccg aataaagtgc aaaaaactcc
      361 ctggcagcac ttaatattgc agccctgtaa aactggttaa aattgctcac aatggacatt
      421 tcttaacgtc accccaagca taatcacagg tagcaagtca gatgtcaaac accacaagaa
      481 agcccctagc gctgctccca cagctcttag cagccacgct aacagtcaca gccgccgccc
      541 aggccgcccc ggtctggaat atcgattctt cccactcgga cgccggattt tccatcaagc
      601 acttgatgat ttccaacgtc aaaggctcct tcacaaacgt aaccggtaca gttgaatacg
      661 acggcaaaga tcccagttcc atcaaggttg atgccaccat tgataccacc actgtcaata
      721 ccggtgacaa aggtagagac gagcatctgc gcggcgccga cttcttcgat gccgagaaat
      781 ttcccaagat gactttcaaa tctaagaaag tcaaatccct gggcaagggc aagtttgcac
      841 tcaccggcga tctgaccatc aagggcatca ccaaagaagt aaccctgacc gtagatggac
      901 cctcaaaaga agttaaagat ccctggggca atactaaaat tgccgctagc gcttccacaa
      961 aaattcagcg caaagacttc aacatcacct acaacaaagc catggataac ggcggcaccg
     1021 tcatcggtga cgatgtagcc attaccctgg aaatcgaagc caacaagaag caataaatcg
     1081 ttgaccaaaa tccggaaacc gaaaatatgg aagcgcctct gtcttttggc agcggcgctt
     1141 tcctgtgttt cagccagtcc ggcggcagag caggatagcc tcaagcaggc agtggcactt
     1201 ttcaacaaag gtgactatgc cgcatgtacg gccctgatta accgagatat caacggctct
     1261 ctcaagaata atgccgccgc tcattattat ctgggcacgg ccctgctccg actgaatcaa
     1321 aacaatgccg ccgctgagca ctacctgtcc acaagcaagc tagcgccggg caccgtatac
     1381 gatgactata gccgcaaggc ccttaaatca ataatggcta gagccggcaa catgcccaac
     1441 tccttgaaag agcagtacca ggccctgacc aacccggggc gcaccggaca aacagccgct
     1501 actaactcac agtcaaccaa ctcgcagtca accaactccg gctacaggca aactgaagac
     1561 gacgaccaga cccttcccac tgccgccctc aaggacgtag acagcacctt catcagcacc
     1621 ggcaggcgca ccgccgacac cgaccgcact ctcgtgcagg tgatgagagc cattaaactg
     1681 gttcccaaaa acatagtcga cgaactgaaa gcgggcggca tctcagtact ggtgacgccg
     1741 accatcttgg aagcaatgcc tggttcagcc atggacaaac cgagaggtta tgaccatggc
     1801 ggcggctaca caaattgcgg cgcactcttc cacagtcccc gcattttcat cggtgagcgg
     1861 gtatcttatc tatccagcgt tccctacctc aataggaaca tcgcctcaag catgctccat
     1921 gaaatgggcc atgcctacga cagcgtcaaa ggacacctgt cgcagtcagg cgaatttgac
     1981 cgatgctaca aggaagactt ctcgcacatt accaacaccc ag
//
LOCUS       DAOQNZ010000793         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37222_length_2020_cov_4.176590, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000793 DAOQNZ010000000
VERSION     DAOQNZ010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37222_length_2020_cov_4.176590"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PKW73_13440"
     CDS             complement(<1..505)
                     /locus_tag="PKW73_13440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA74342.1"
                     /translation="MSIQSVCTNRPRSGAARFCLKQVGLPVSTLVILLCTATEAYGQV
                     DIYAPSTSECPPSVLSPGVSDQPNRPVNRFSLTLSRAFMEANATNPDVVTAAKQLEIA
                     QAELKTAAAIPNPQLAVQYGFGTPYTHVVAGNTQQVGINQLIEMGGKRGARIKLAQAN
                     LQLAQFRL"
     gene            complement(625..>2020)
                     /locus_tag="PKW73_13445"
     CDS             complement(625..>2020)
                     /locus_tag="PKW73_13445"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNA74343.1"
                     /translation="EPGKTFLGLGFRAELAWFIERHQGISFVEILAEDFPSPGQIPDA
                     LKRLHRRGIEIIPHSTSLSLGSTTLPSRQLLKHLDELARYFDAPFVSDHIALVRAGGL
                     ESGHLLPVQRSQAMVELLKHNLAEARAGLSVPLVLENIATTFDWPVEHNEMGEAQFLR
                     RILEETDSKLLLDVSNLFANSFNHHFDAGSFLSQLPLERLEYVHLAGGAFKQGLYHDT
                     HCHPLREESLKLLSLLAGLCRGANLPLKRVMLERDDNFAEDDELAAELAAIEAACGGP
                     SEVMPPPIVRPPNRALPERAPELLTDTPEVNAGHCRSAQTIEKIEAGHRALLAALLRE
                     PQGAGEGVVPGLPPELSGFDGKLLGQARQSLIAKRLRTMKRACPYLMEIFPREEMLDQ
                     ALRRFYRDSPSPSAAGPYTDAMHFLVFLQGKSEEFAGLDGYKLTPQAVKQARRHLSRG
                     SFWQSLIQGLFPAK"
BASE COUNT          490 a          545 c          592 g          393 t
ORIGIN      
        1 tgaggcgaaa ctgagccagt tgcaaattag cttgcgccaa tttgatgcga gcaccgcgct
       61 tgccgcccat ttcaatcaac tggttgatac ccacttgctg ggtgttgcct gccactacat
      121 gggtgtaagg cgtaccaaag ccatattgca cggcgagctg agggttggga attgcagcgg
      181 cggttttcag ctccgcctgg gcgatctcaa gttgcttggc ggcagtaacc acatcagggt
      241 tggtggcatt agcctccatg aaagcccggg aaagcgtcag ggagaagcga ttgacaggcc
      301 ggttgggctg atcggataca cccggcgaca gtacactggg cggacattcg gatgtagaag
      361 gggcatatat atcaacctgc ccgtaagcct cggtagcggt gcacagcagt atgaccaggg
      421 tagagaccgg caagccgacc tgcttcagac aaaatcttgc ggcaccggag cgaggcctat
      481 ttgtgcatac ggattggata gacaaacgat cccttaagaa gactgcgaca cggactgtca
      541 aattttaacg gcaatttgtg atcaaagtgt ttgcagaaaa gtaagaagac tgccgctctt
      601 gccaagtccc catcaattcc cgccctattt tgcggggaac aagccctgaa taaggctctg
      661 ccagaaggaa cctcgggata aatgccgccg ggcttgcttg actgcttggg gcgtcagctt
      721 atagccgtcc agaccggcga attcctcact tttaccttgc agaaagacga gaaagtgcat
      781 ggcatcggtg tatggtccgg cggcagaagg cgagggcgaa tctctgtaaa agcgcctcaa
      841 agcctgatcc agcatttctt cccggggaaa aatctccatc aggtaggggc aggcacgctt
      901 catggtgcgc agccgcttgg caatgagcga ctgcctggcc tggcccaata gcttgccatc
      961 gaagccggac agctcaggag gcaggccggg caccacacct tccccagccc cttgcggctc
     1021 ccgcaataag gcagcgagaa gagccctgtg accggcttca attttttcga tggtctgggc
     1081 agagcggcag tggcccgcat ttacctccgg cgtatcggta agaagctcgg gcgctcgctc
     1141 aggaagggcg cggttcggcg gtctgactat gggtggcggc atcacctcag acggcccgcc
     1201 gcaagccgct tcgatggcgg ccagctcggc ggccagttca tcatcttcgg caaaattatc
     1261 gtctcgctca agcataacgc gcttgagagg cagattggcg ccacgacaaa gcccagccag
     1321 aagggaaagc agcttgagcg actcttcccg cagtggatga cagtgagtgt cgtgatagag
     1381 cccctgcttg aaggccccgc cggctaagtg tacatactcc aggcgctcca gaggcaactg
     1441 actgagaaag ctaccggcgt caaaatggtg attgaaagaa ttggcgaaga gattggaaac
     1501 atccagaagc agcttgctat cagtctcttc aagaatgcgc cgcagaaact gcgcctcccc
     1561 catttcgtta tgctcgaccg gccaatcaaa tgtggtggca atgttttcca aaaccagcgg
     1621 cacactcagc ccggcgcgcg cctcggccag attatgcttg agcagttcaa ccatggcttg
     1681 actgcgctgt acgggcagaa gatgaccact ttccagacca ccggcccgca ccagggcgat
     1741 gtgatcgctg acaaagggag catcgaagta gcgggccagt tcatcgagat gcttgagcag
     1801 ttgccgagaa ggcaaagtag tggaaccaag agataaggac gtggagtggg ggatgatctc
     1861 gataccacgc ctgtgcaagc gcttgagagc atcaggaatt tgaccgggtg agggaaaatc
     1921 ttccgccaga atttccacga aagaaatacc ctgatggcgc tctataaacc aggcaagctc
     1981 ggcacgaaag ccaagcccca ggaaagtttt gccaggctcg
//
LOCUS       DAOQNZ010000794         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37402_length_2015_cov_2.861735, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000794 DAOQNZ010000000
VERSION     DAOQNZ010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37402_length_2015_cov_2.861735"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(618..1892)
                     /locus_tag="PKW73_13450"
     CDS             complement(618..1892)
                     /locus_tag="PKW73_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA74344.1"
                     /translation="MSDNEFQVQFAKQDIPMRLILTFPSLQQVLKAEKLLRTAESQFS
                     CRTTPTPPGLSADICGMSLELLEPGLKDEVVAFLERKDLVPMGVHSLESKEARVPTRT
                     TAVLVDSFAPLTEEHLYRIDFALNLADSVLVLLLEDMSQVGMKPIPVSLRLNWLKESR
                     PDIEVKICADKESALAYLQQLSTAQGQSTGRKFDFFLSADKGDNAVADNLSARFVPTD
                     PGGELFQRDSSARFDPIKNFDKLAPAARLTYLKRVCIFGPESTGKSTLTKNLARHFQT
                     RFVPEYARTFMEAKNNQLEQQDLALFAAGMAASENALAHTANRVLFSDTDALTTCIWS
                     EWLYKEVPEELLALAQRQKHDLYLLLDVDVPWIEDPQRYYPEERETFLASCKLYLERW
                     QRPYQVINGSFEERLEKSIMLVSDLLNRVTEP"
BASE COUNT          444 a          483 c          572 g          516 t
ORIGIN      
        1 caccggtggt tgccgccgtg ggggcgggct tcgttttgac gtttttcatg gttacctccg
       61 tagggcaccg atagtgggat tatatcgatg cctggttaga tacataaaga gagaccacaa
      121 gagcaggctt ctgatggtca tggctgacgc ctgccacttt tgcttgcttg cggtgcgacg
      181 cgacaaactg attcccggga cggagtcctg ggggagaatg gttgaaagtc tgagtttctg
      241 attgaggtta agcttgaggg cctggttgag tttatgggtg caaaaataga ctttgagctt
      301 attcgtttcc aagcgctaag ctcaaggtcc tgagaaaacg cctcaatagt ttctggcagc
      361 aaaaacactt ctatctctta ttaacagagt ctgttttcta acagctgcgg gctttgtggg
      421 gatgaatgac cggatctgtc agcccggatg gttgatttaa ggttgctcat cttgacatct
      481 gtcggttagc gatactcgcg caaggtgtta accggccgat ggcagaggcc tgcccagccc
      541 aactgtggaa gttgttaacc gttatcggtt aacagcacaa tcgggtcggt gtcgtcgacg
      601 ggccgcgggt cgccttttca gggttccgta acccggttca gtaggtcgga cacaagcatg
      661 atggactttt ccagcctttc ttcgaagctg ccgttgatca cctggtatgg tctttgccag
      721 cgttccaggt acagcttgca ggaagccaga aaagtttctc tctcctcggg atagtagcgc
      781 tgtggatctt ctatccatgg cacatccaca tccagcaaaa ggtaaaggtc atgtttctgc
      841 cgctgggcca gggcgagaag ctcttccggc acctctttgt agagccattc actccagata
      901 caggtggtaa gggcatcggt gtcggagaaa agcacacgat tggcggtgtg agcgagggca
      961 ttttcagagg cggccatgcc ggcagcaaaa agggccaggt cctgttgctc caactggtta
     1021 ttctttgctt ccataaaggt gcgggcgtat tcaggcacga agcgggtctg aaagtgcctg
     1081 gccaggttct tggtgagggt acttttaccg gtggattcgg gaccaaagat acaaacccgt
     1141 ttcaaataag tcagacgcgc tgccggtgcc aacttgtcaa agttcttgat gggatcaaac
     1201 cttgctgagg agtccctctg gaagagttcg ccgcctggat cggtgggcac aaatcgggca
     1261 gaaaggttgt cggcaacggc attatcgccc ttgtctgcgg acaggaaaaa atcaaattta
     1321 cgtccagtgc tctgcccttg tgcagtgctt aattgctgca gataggccag tgccgattct
     1381 ttatcggcgc agatttttac ctcgatgtcg ggacggcttt ccttgagcca gtttagtctc
     1441 aagctgaccg gaattggttt cattccgact tgcgacatat cctctagaag caagaccagt
     1501 acagaatcag caagattgag ggcaaaatcg atacggtaca ggtgttcttc cgtgagaggc
     1561 gcgaagctgt ccaccagcac ggctgtggtt ctggtcggaa ccctggcctc cttgctttcc
     1621 aggctatgca cacccatggg taccaggtct ttccgttcaa ggaaggcgac cacttcatcc
     1681 tttaagccgg gctcgagcaa ctccagggac ataccgcaaa tatcggcgga tagaccgggc
     1741 ggggtcggcg tcgtgcgaca actgaattgg ctctcggcgg ttcggagaag cttctctgcc
     1801 ttcaaaactt gttgcaaaga tggaaaggtc agaatcaagc gcatcgggat gtcctgcttg
     1861 gcgaattgaa cttggaattc attatccgac atactcagag ggacggcttg ctaactttgc
     1921 tgaatttgct gaacagctca cctgttttgt cgtcaaattg ggcctaattt ttgaccggtg
     1981 ccggttttca caaagagcgg gaagttcctg ctgct
//
LOCUS       DAOQNZ010000795         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37414_length_2015_cov_1.891327, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000795 DAOQNZ010000000
VERSION     DAOQNZ010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37414_length_2015_cov_1.891327"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="PKW73_13455"
     CDS             complement(<1..1463)
                     /locus_tag="PKW73_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74345.1"
                     /translation="MGGNTLGDQMLVNATEDLNGVVNQGKQVADQARERLSLSGQMLQ
                     GAIPEGAANLPSEDIGAVERYGTVFGKGLLRTPEGVFNTLGHYWDNPGEAAGKALFAG
                     SIGVAMRTFLPKTGAGRAAIATIMGGMFVMDGVKALAQGGSEASAARNRQELNQAATN
                     FGNHMGMFAFDAAAGGYIGIKAERFTGKILENPVGTAKTVGGWADSVVGRFGKTAPAA
                     EGGEAASAANSYSSRMGAWVEGKEIAPKLAAWDKKVERFWTSDESRVGNFLNRTTEKI
                     DNWASRFKPKEEPVAKNPLANIPAEQAKALMEQAQRQHIEAVDSHMVYRHGLKGADGE
                     YHGFDRTLALLKKGINPEQVGAKDPVPNVPDLVNPKLDEALAGMMKTREQVMGLTEGM
                     AKIADEHGTGQQFKKSEGGTVVEPHEKPGTKPYDKTPQQAAADAEINVANLQEMAKVT
                     AEERAAITAEDTAVIAYKQQMEGQVYTAVVPNAQRNPL"
BASE COUNT          399 a          549 c          531 g          536 t
ORIGIN      
        1 agagggttgc gctgggcatt cggcacgaca gcagtgtaga cctgaccttc catctgctgc
       61 ttgtaagcaa ttactgcggt gtcttcggcg gtgatagcgg cacgctcttc ggctgtgact
      121 tttgccatct cctgcaaatt ggcgacattg atctctgcgt cggcagcggc ttgttgcggt
      181 gttttgtcgt atggtttggt gcccggcttc tcatgcggct cgacgacagt tccgccttcg
      241 ctcttcttga actgttgacc agtcccatgt tcgtcggcaa tttttgccat gccttcggtg
      301 agtcccatga cttgctctct tgttttcatc atgccggcga gagcttcgtc gagtttcgga
      361 ttgaccaggt ccggtacatt cggcaccggg tctttggcac cgacttgctc ggggttgata
      421 ccctttttca gaagggcaag agtacggtcg aatccgtgat attcgccgtc agctcctttc
      481 aagccgtggc gataaaccat atgcgaatcc acagcttcta tatgttggcg ctgagcttgt
      541 tccataagag ctttagcttg ctcggcagga atattggcga gaggattctt ggcgaccggt
      601 tcttctttgg gtttgaaccg tgatgcccaa ttgtcgattt tctctgtggt gcgattgagg
      661 aaattgccga ctctggattc atcacttgtc cagaatctct ctaccttctt gtcccaggct
      721 gccagtttcg gagcaatctc ttttccttct acccaggcgc ccatgcggct ggagtaagag
      781 ttggcggctg atgcggcttc ccctccttcg gctgcaggag ctgtttttcc aaatctgcca
      841 acgacactgt cggcccagcc gcctactgtc ttggcggtgc cgaccgggtt ttccaaaatc
      901 tttcctgtga agcgctcggc ttttataccg atatagccgc cggctgcagc atcgaaggcg
      961 aacatgccca tatgattgcc gaaattagtg gctgcttgat tcaattcctg tctgtttctg
     1021 gctgccgatg cttcggagcc gccctgcgcc agagctttga cgccgtccat gacgaacatg
     1081 ccgcccatga tggtggctat agctgctcta cctgcgcctg tcttgggcag gaatgtgcgc
     1141 atcgccacac cgatagaacc cgcgaaaagt gcctttccag ccgcttcacc cgggttatcc
     1201 caatagtgtc cgagagtatt gaatacgcct tccggtgttc tcagtagacc ctttccgaag
     1261 acggttccat aacgctcaac agcacctata tcttctgacg gaaggttggc ggcaccttcg
     1321 ggaatcgccc cctggagcat ctgacctgac aggcttaatc tttcacgagc ctggtcggcg
     1381 acctgctttc cctgattaac cacaccgttc aaatcctcgg tggcattgac cagcatctga
     1441 tcgcctaacg tgttcccgcc cattcagacc tccagcgttt ccgatgtaac ccagtgttac
     1501 gagagatttc agacaggcaa gaccgtgtcg cccggaagtc cattccgggt cgcgagggag
     1561 gttttgcttc gaatttcctg ggggtgaacc tgtggaaatc ggtcttaatc cgtgccgtca
     1621 cccatatctt ccagctattg ggggtagcgg cgattcactc cttaagacta tctcaggacg
     1681 gtaccgcgtg tatattcggg aaattacggt cgcttgcgta agaatcccac cccgacatta
     1741 aaatcggcgt ctgtcataat tgtgtgccgt ggccacaggc cgatttagat cctggcggaa
     1801 ttaggcgcag ttgccgtttc ccgccttata caactgattt agaggcgaaa aagtggagaa
     1861 aaaacctcag ggttccagtg gatctttgaa aggaaaaatt tccaaagaag aactgaagga
     1921 caaaattaga ggagtgattc tggctgcagc ttgcggcaac tcgctgggcg gcagcgcaat
     1981 cgggctgaac cataaagaga tattggccac agccg
//
LOCUS       DAOQNZ010000796         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37467_length_2013_cov_3.287538, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000796 DAOQNZ010000000
VERSION     DAOQNZ010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37467_length_2013_cov_3.287538"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKW73_13460"
     CDS             <1..256
                     /locus_tag="PKW73_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74346.1"
                     /translation="HLGEAPARVVAKAAPRPVFLLGGGNDVIVPEQLNQTTWQALFQS
                     HDYPTRCSSLPRADHGFSLEEDVFACISSLCEFLSQASAN"
     gene            587..>2013
                     /locus_tag="PKW73_13465"
     CDS             587..>2013
                     /locus_tag="PKW73_13465"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNA74347.1"
                     /translation="MTANILDSLHAPLTFQLLSIALEAARSAGATYCDARILKRQSQF
                     IQTKNDKLLALNQEESTGVGIRVLANGGWGFCATQNLSEKSLSRCAQQAAAIARASGS
                     CRREAIKLIPEPAWRATWTSPHLIDPFTVELDTKLDLLLSCAETILKVPGITLAQGML
                     HFIKDRQFFANSEGAQIEQLSIRSGCLIEATAAADGDQQRCSYPCSFGQYEQSGYEIV
                     EKWNLPAHAEEIARRAVALLSAPTCPRGEIDTIIGASQLGLQIHESMGHPSELDRALG
                     QEINFAGASFLTPDKLGKLRYGSKLVNLVADATASGALGSFGFDDEGVQAQCTYLVKD
                     GVFKGYLSSRDTAALVGLRRSGGAMRAESWNYAPIIRMTNISLVPGEAGSLEDLIADT
                     KDGLYMETNKSWSIDSMRYNFQFSTEAAWEIKNGKLGRLLKNPSYSGITPDFWNSCDA
                     IAGRSHYTEWGTPNCGKGQPMQTMWT"
BASE COUNT          488 a          602 c          504 g          419 t
ORIGIN      
        1 ccacctggga gaagcaccgg cacgggtagt ggcaaaagca gcaccgcgac cggtgttttt
       61 gctaggcggc ggcaacgatg tgattgtgcc ggaacagcta aatcagacaa cctggcaggc
      121 gctctttcag agtcacgact acccgaccag gtgctcaagc ttgccaagag cagatcacgg
      181 tttcagcctg gaagaggacg tgttcgcatg cataagctcg ctgtgcgagt tcttaagcca
      241 ggctagcgcc aactgagacg cacggttaca aagtccgaaa ggtcgcccac gcagccattg
      301 accctggcgt ttctttcaaa cagccaccag tattagttat tagtaacaag gttttgaatt
      361 cagagcgttc ttacaaactt gagcttattc ctctggaatt cgtaatgtcc ttctaaaccc
      421 tgttttcttc ccccgagaaa aaaatccccc gaaattccgc tcttttctac tttcattgaa
      481 agtcttcacc ctttaaggaa aggtcgcagc aaaaggtctt ccatgacgga aatcaagctg
      541 gtaaattcag cttaatctca tcgatacagt ggaagccagc tctaccttga cagccaacat
      601 tctcgacagc ctgcatgccc cgcttacctt ccagttgcta tctattgccc tggaagccgc
      661 ccgaagtgcc ggagccacct actgcgatgc ccgcattctc aagcgtcagt ctcagtttat
      721 tcaaaccaag aacgacaagc tcctggccct caatcaagaa gaaagcaccg gcgtaggcat
      781 ccgcgtcctg gccaatggag gttggggctt ttgcgccact caaaacctct cggaaaagtc
      841 cctctctcgc tgtgctcaac aagcagccgc catagccagg gccagcggtt cctgccgcag
      901 agaagcgatc aagttgatac cggaaccggc ctggcgtgcc acctggactt cccctcatct
      961 catcgacccc ttcaccgtag agttggatac caagcttgac ctgcttctct cttgcgctga
     1021 aaccatcttg aaagtgccgg ggatcacact cgctcaaggc atgctgcact tcatcaaaga
     1081 caggcaattc tttgccaaca gcgaaggggc gcagatagag caactctcca tccgctccgg
     1141 ctgcctcata gaagcaaccg ctgccgcaga cggcgaccag caacgctgtt cctatccctg
     1201 cagcttcggt cagtacgagc agagcggcta cgaaattgtc gagaaatgga atttgccagc
     1261 ccatgccgaa gagatagcca ggcgcgcagt ggcgctactt tcagcaccga cctgtccacg
     1321 gggcgaaatt gacaccatca tcggcgcttc ccaattgggt ctgcaaatcc acgagtccat
     1381 gggacatccc tccgagttgg accgcgccct cggtcaggaa atcaacttcg ccggcgcctc
     1441 cttcctgacg ccggataaat taggcaagct gcgttatggt tccaaactgg tgaacctcgt
     1501 agccgacgcc actgctagcg gtgcgctggg aagtttcggc tttgatgacg aaggtgtaca
     1561 agctcagtgt acctacctgg tgaaagacgg cgtcttcaag ggctatctga gctcccgaga
     1621 cacggctgcc ctggtgggac tgagacgctc cggcggagcc atgagggcag aatcctggaa
     1681 ctacgctccc atcattcgca tgaccaatat ctccctggta ccgggtgaag caggcagtct
     1741 ggaagacctg atcgccgaca ccaaggatgg tctgtacatg gagaccaaca agtcctggtc
     1801 catagacagc atgcgctaca acttccaatt ctccactgaa gccgcctggg agataaagaa
     1861 cggcaaactg ggcaggctac tcaagaatcc cagttacagc ggcatcaccc ccgacttctg
     1921 gaattcctgc gatgccatcg ccggacgcag tcattacacc gagtggggta cccctaactg
     1981 cggcaaaggt cagcccatgc agaccatgtg gac
//
LOCUS       DAOQNZ010000797         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37598_length_2009_cov_4.213408, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000797 DAOQNZ010000000
VERSION     DAOQNZ010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37598_length_2009_cov_4.213408"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKW73_13470"
     CDS             complement(<1..432)
                     /locus_tag="PKW73_13470"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HNA74348.1"
                     /translation="MTKKRRLNLIDFSLLAGALTAVLGLGLAKAGYAGVNQKIQGTPR
                     VNIEVFITGLKTKDTTLFTPGQKASITIRNQPVHPPMTIVKCEHWQKQVAFLSPDGKK
                     ALAFPDPANAIAHDFLVTVQDDSEQTEDGYVIRGNKIKVGNQ"
     gene            complement(436..696)
                     /locus_tag="PKW73_13475"
     CDS             complement(436..696)
                     /locus_tag="PKW73_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74349.1"
                     /translation="MPEISLEMSAMDLLKLANDFHTGKLTLSAEEKSHLLDLLRIKGA
                     EELEYGQEVEHEKVRLVRRVTGAVSIFFEKEAEPSAKKKAKE"
     gene            885..1250
                     /locus_tag="PKW73_13480"
     CDS             885..1250
                     /locus_tag="PKW73_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74350.1"
                     /translation="MAQFENESKHVEKAKLDANREGGENHGDKLVKAAYGDQGEPGIM
                     CNDNTRELLRQNEAFIKQVQEQAERVVASIMKSFDALPMEKQEQFVNGIDRKNLSKHD
                     VVYHTFLRNWDSRQTSASA"
     gene            complement(1273..>2009)
                     /locus_tag="PKW73_13485"
     CDS             complement(1273..>2009)
                     /locus_tag="PKW73_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNA74351.1"
                     /translation="LEAHGITHDLVKDKTRPTTVKTRIISKSHSLMQQLLRIDRISHE
                     PISRESEAALIEKINNAQNVETHAIILSDYKAGVITDGVIAAVKKMAKEKDLMVIVDA
                     QGDFQRFQDCTLMTPNQPDAEAYAQMEINSREALEKLGEILLNKSGVKALLVTRGGEG
                     MVLFQKGKPMFEIPAFNRSEVFDVTGAGDTVVATMALALTSGASMEEAMALGNLAASI
                     VVRKPGTAVTSQKEMLEHLEKLNLPD"
BASE COUNT          436 a          473 c          549 g          551 t
ORIGIN      
        1 ctggttgccc accttgattt tgttgccgcg aatcacgtaa ccgtcttcag tttgctcgga
       61 atcgtcttgc acggtgacca ggaagtcgtg agcaatggca ttggccgggt cgggaaaagc
      121 cagggctttc ttaccgtctg ggctgagaaa ggctacttgt ttctgccagt gctcgcattt
      181 gacgatggtc atgggcggat gcaccggctg gttgcgaatg gtgatgctgg ctttttgacc
      241 gggggtgaaa agggtggtgt ccttggtttt gagaccggtt atgaaaactt ctatgtttac
      301 cctgggagtg ccttgaatct tctggttgac tccggcataa ccggccttgg ctagacctag
      361 accgaggacg gctgtcaaag ccccggcaag caggctgaaa tcgattagat ttaggcgtct
      421 ctttttggtc atggcttact cttttgcttt cttcttggcg cttggttcgg cttctttctc
      481 gaagaatatg gaaaccgcac cggttactct tcttaccagt cgtacctttt catgttcgac
      541 ttcctgccca tattctaatt cctccgcccc tttgattcgt aaaaggtcaa gaagatgact
      601 cttttcttcg gcactgaggg tgagtttgcc tgtgtggaaa tcgttggcca gcttgagcag
      661 gtccatggca ctcatctcta acgatatttc tggcattggt tttcttgttt agaagagcta
      721 gcacatatta gcagcttgac caccggacgc gcttttcaaa tttttgcaga tatttttgcg
      781 gtaaatacgt agcttcatgt cgaagaattc tctcaggaag atacatccat acacgtggcg
      841 atgaccgagt caagaaatgc tcttacccga aggaatttaa gaaaatggcg caatttgaaa
      901 atgaaagcaa gcatgtcgag aaggcgaaac tggatgctaa ccgcgaaggc ggagagaatc
      961 acggcgacaa actggtcaag gcggcttacg gcgatcaggg cgaacctggg attatgtgta
     1021 acgataatac ccgtgagctt ttgcgccaga acgaagcctt catcaagcag gtccaggagc
     1081 aagccgaacg tgtagtggcc agcatcatga aaagttttga cgctctgccc atggaaaagc
     1141 aggagcagtt tgtaaacggc attgaccgca agaatttgag caagcatgat gtggtttacc
     1201 atacttttct gagaaactgg gattcccgcc agactagcgc ctctgcctga ggcttacggc
     1261 aggttcactg cctcagtcgg gcaaattgag tttttccaga tgctcgagca tttctttctg
     1321 gctggtgacg gctgtgcctg gttttcttac gacaatggaa gcggccaggt ttcctagagc
     1381 catcgcttct tccatgctgg caccggaagt gagcgccagg gccattgtgg caacaacggt
     1441 gtcacctgcg ccggtgacgt cgaagacctc cgagcggttg aacgccggaa tttcaaacat
     1501 gggtttgccc ttctggaaga gcaccatgcc ctcaccgccg cgggtgacga gcagggcttt
     1561 tacccccgac ttattgagca gaatttcgcc cagcttctcc agtgcttctc tggaattgat
     1621 ttccatctgg gcataagctt ccgcatcagg ctgattggga gtcatcaagg tacagtcctg
     1681 gaagcgctgg aaatcaccct gggcgtcgac tataaccatc aagtcttttt ctttagccat
     1741 tttcttgacg gcggcgatga caccatcggt aatgacaccg gctttatagt cggagagaat
     1801 gatggcgtgt gtttcaacgt tttgagcgtt gtttatcttt tcaatgagag cggcttccga
     1861 ctctcgcgag atcggttcgt ggctgattct gtcgatgcgc agtagttgct gcatcaggct
     1921 gtgagattta gagatgatgc gggttttgac cgtggtcggt cgggttttgt ctttgaccag
     1981 gtcatgggta atgccatggg cttccaggc
//
LOCUS       DAOQNZ010000798         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37642_length_2008_cov_4.180236, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000798 DAOQNZ010000000
VERSION     DAOQNZ010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37642_length_2008_cov_4.180236"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKW73_13490"
     CDS             complement(<1..355)
                     /locus_tag="PKW73_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA74352.1"
                     /translation="MWIVEMALKRAHTFIVMALAILIFGILSIRQMAVDIFPIIDVPI
                     VSAVYTFTGMSPYNIENLITTVTERWLTSTVNGIEKIESTSLSGMSIIKVYLHKGTDI
                     GEAVAMVTAMGSAVLE"
     gene            complement(355..>2008)
                     /locus_tag="PKW73_13495"
     CDS             complement(355..>2008)
                     /locus_tag="PKW73_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA74353.1"
                     /translation="AVRLFYRGLLDKVLDHPLISITSFLAVYAALLSLIPLIGQDYFP
                     AIDGGQLRMHVATHAGTRVEVTEHIFKQVEKAVKEVIPEDEIASISDNIGLPCSGINY
                     AYSDSQTASEADGEILISLKEERAHDTEYYQKQIRKLMRSRFPDLTFYFQPGDIVTQI
                     LNAGLPAPIDIKVIGYNKNNYQLAKDIKRKIEKVPGAVDICLHQITDGPHLRWTVNRT
                     FAQEAGVTQADVSNSFLINLSNSFQTNPNFWVNQKTGISYNLAVQTQQRDLATMNDIG
                     LTQITGGTTGINPSVSGDPQPTSGLKQGDSPRRGQLLMNLATFERARTPLVINHINVQ
                     PVYDIYAACQDRDLGGVSQDIAKILEEVKKNLPRGAKVVMMGQVLSMNLAFLALLLGL
                     VFALVLVYLLLVINFQSWSDPLIILMAIPGALSGIVLSLFLTQTTFSIPALMGTIMTM
                     GVASANSILMVTFARGELEETKDARLAALNAGFERFRPVIMTASAMIIGMIPMALGGG
                     EGGSQNAPIGRAVIGGLSVATISTLFFVPLIFYILRRKGGGK"
BASE COUNT          478 a          494 c          552 g          484 t
ORIGIN      
        1 attcgagcac cgccgagccc atggccgtca ccatggccac cgcttcacca atgtcggtgc
       61 ctttgtgcag gtagaccttg atgatgctca tgccggaaag agaggtagac tctatctttt
      121 cgatgccgtt tacggtggag gtgagccaac gctcagtgac cgtggtaatc aggttttcga
      181 tgttataggg cgacatgccc gtgaaagtat agacggcact gactatgggc acgtcgatga
      241 tggggaaaat atccacagcc atctggcgga tggaaagtat gccgaaaatt agaatggcca
      301 gagccataac gataaacgta tgggccctct tcaaagccat ttccactatc cacattactt
      361 accacctccc ttgcggcgca aaatatagaa tatgagcgga acaaaaaaca aagtggaaat
      421 ggtggcaacg gaaagaccac cgattaccgc tctgcctatg ggagcattct gggagccccc
      481 ttcgccgcca cctaaagcca tgggaatcat gccgatgatc atagcggaag ccgtcattat
      541 aaccgggcgg aagcgctcga atccggcgtt cagggccgcc agcctggcgt ccttagtttc
      601 ttccagttcc ccccgggcga aggttaccat caaaatggag ttggccgagg ccacacccat
      661 ggtcataatg gtgcccatga gtgccggaat ggaaaaagtt gtctgggtga ggaaaagact
      721 gagcacaatg ccggagaggg caccgggtat ggccatcagg atgatcagag gatcactcca
      781 gctctggaag ttgattacaa gtaagagata taccagcacc agggcaaaga ccagacccaa
      841 gagcagtgca agaaaggcca gattcatact caagacctga cccatcatca ccactttcgc
      901 tccccggggc aggttctttt tcacttcctc cagaatcttg gcgatatctt gagagacgcc
      961 acccagatct ctatcctggc aagcggcata gatgtcgtaa accggctgta cgtttatgtg
     1021 attgattacc aggggggtgc gtgccctttc aaaggtagcc agattcatta agagctgccc
     1081 acgtctgggg ctatcgccct gtttaagacc gctggtgggc tgagggtcgc cgctgacgga
     1141 gggattaata ccggtggtgc cgcctgttat ctgggtcagt ccgatgtcgt tcatggtggc
     1201 aaggtctctt tgctgagtct gtaccgccag gttgtaactg atgcctgtct tctggttcac
     1261 ccagaagtta ggattggttt gaaaactgtt gctcaaattg atcaagaagg agttggaaac
     1321 atcggcctgg gtgactcccg cttcttgtgc aaaggtgcgg ttcaccgtcc agcggaggtg
     1381 cggtccgtct gtaatttgat gcaggcaaat atctacggcg cccggaacct tctctatttt
     1441 tcgcttgatg tctttggcca gctggtagtt gttcttgttg tagccgatta ctttgatgtc
     1501 gatgggggca ggcagtccgg cattgagtat ctgggtgacg atatcaccgg gttggaagta
     1561 gaaagtcaaa tcgggaaagc ggctacgcat cagcttgcgt atttgctttt gataatactc
     1621 cgtatcatgg gcccgttctt ccttcagtga aatcagtatc tcgccgtcgg cttccgaggc
     1681 ggtttgactg tctgaatagg catagtttat tcctgagcag ggcaacccga tgttatccga
     1741 tatagaggca atctcatctt cgggaatgac ttctttgacg gccttttcca cctgcttgaa
     1801 aatgtgttcg gtaacttcca ctctggtgcc ggcatgggta gccacgtgca ttcgcaactg
     1861 cccaccatcg atagccggga aataatcttg accgatgagc ggtatgagcg atagcaaggc
     1921 tgcatagact gccagaaagg aggtaatgct tatgagcgga tgatcgagca ctttatccag
     1981 gagcccgcgg tagaaaagcc ttacggct
//
LOCUS       DAOQNZ010000799         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37685_length_2007_cov_3.876025, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000799 DAOQNZ010000000
VERSION     DAOQNZ010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37685_length_2007_cov_3.876025"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1901)
                     /locus_tag="PKW73_13500"
     CDS             complement(195..1901)
                     /locus_tag="PKW73_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNA74354.1"
                     /translation="MLRLDNVCKIYPHGEVLKEVSWEVKPGSRIGLVGANGSGKTTQF
                     KIICGHEEPTSGQVVRAQGLKIGYLCQEFDLVPTNTVAEEFMRAFVELNEIQGRLDLV
                     HKAMETAQDKELNRLIREMDDLQRQFEAKGGYTTDAKIEKMLVSMGFGAEGGKRLVRE
                     FSGGWQMRMNLGKVLLREPDVLLLDEPTNHIDLETIEWLESFLVSNSTPLVMVSHDRH
                     FLDRLCTSIVEMERGTATQYLGNYTQYVEARALNRDAQLAAFERQSREMEKQQAFVDR
                     FRASATRSTQAKSREKQLDKIELVDKPDTEERTLEFHFPQAVRSGMEVATIKNLTHAY
                     GDNVIFLDANLHITRGDRIAILGPNGSGKSTLLRILTGAEPALSGQAALGAHNVKMAY
                     FEQNQAEALDLTKSVLDTIYDEVPEWKTDEVRGLLGRFLFSGDAVFKNVATLSGGEKA
                     RLALAKMLLGANNFLILDEPTNHLDIPAKETLEAALKKFDGTVVVVSHDRYFIQEVAN
                     KIVEIKDGQLVLYHGNYHYYLERKEKERLKEEEKRLAAEKAEKDAQKRAQRLAKEKGR
                     KA"
BASE COUNT          422 a          562 c          521 g          502 t
ORIGIN      
        1 ggcaggcgct tcttaggaaa acgcagagag cctgatagag aactgaggta ttgatcgggc
       61 tgactctgag cagaggctga acaggaaaga aacgcacaaa gcaaaacaag caaaaccgcc
      121 tttcccttgt gagtacgcct cgtcatgcag tctaactcct catcatcggt ggacgaactt
      181 acctcggcgc tcgctcaggc tttccggccc ttttctttgg ccaggcgctg ggctctcttc
      241 tgggcatctt tctcggcttt ctcggcagcc agacgctttt cttcctcttt cagccgctct
      301 ttttctttgc gttccagata atagtgataa ttgccgtggt agagcactag ttgtccgtct
      361 tttatctcga caattttgtt tgccacttcc tgaataaaat agcggtcatg ggaaactact
      421 accacggtgc cgtcaaactt cttgagggcc gcttccagag tctctttagc aggaatgtcc
      481 aggtggtttg tcggttcatc gaggatgaga aaattgttgg cgcccagaag cattttagcc
      541 agagccagac gagccttctc gccgccgctc aaggtagcca catttttgaa gacggcgtcg
      601 ccggaaaaga gaaagcgccc caagagtccg cgcacttcat cggttttcca ttccggcact
      661 tcgtcgtaaa tggtatccag taccgatttt gtcagatcca gcgcttccgc ctgattctgc
      721 tcgaaatagg ccatcttcac attatgggcg cccagggcgg cctgccccga gagcgccggc
      781 tcggcaccgg tcaggatgcg caaaagcgtt gatttgcctg agccgttggg acccagaatg
      841 gcgatgcgat cacctctggt gatgtgcaaa ttggcatcta gaaatatgac attatcgccg
      901 taggcatggg tgaggttttt gatggtggcc acttccatgc cgctgcgaac ggcctggggg
      961 aagtgaaact ccagggtgcg ctcttccgtg tccggcttat caaccagctc aattttatcc
     1021 agttgctttt cgcggctctt ggcctgggtg ctgcgcgtag cggaagcgcg gaagcgatcc
     1081 acaaaagcct gctgcttttc catctcccgg ctctgtcgtt caaaggcggc cagctgggcg
     1141 tccctgttga gagcccgggc ttccacatac tgagtgtaat tgcccaggta ctgggttgcc
     1201 gttcctcttt ccatttccac aatgctggtg cagagccggt ctaagaaatg gcggtcgtgg
     1261 gagaccatga caagaggagt gctgttagag accaggaagc tttccagcca ttcgatggtc
     1321 tcgagatcaa tatggttcgt cggttcgtcc agaagcaaga catcaggctc acgcaagagc
     1381 accttgccca ggttcatacg catctgccaa ccgccgctga attcccgcac caggcgcttg
     1441 ccgccctcgg cgccaaagcc catggagacg agcattttct caatcttggc atcggtggtg
     1501 taaccgcctt tggcttcaaa ctgcctctgg agatcatcca tttcccgaat caggcggttc
     1561 agctctttat cctgggccgt ttccatggct ttgtgcacaa ggtcgagccg gccctgaatc
     1621 tcgttcagtt ccacaaaggc ccgcatgaat tcttccgcca cggtgttggt cggcacaagg
     1681 tcgaactctt gacaaagata gccaatcttc agaccctggg cccgcaccac ctgaccggag
     1741 gtcggttctt cgtgcccgca aattatctta aactgggtgg ttttgccgct gccgttggcg
     1801 cccaccagcc cgatgcgcga gccaggtttc acctcccagg aaacttcctt gagaacttct
     1861 ccgtggggat aaatcttgca tacgttgtcg agcctgagca aagctacctc tggtgatacc
     1921 gctaaccaaa caaatctatt gaaatctaaa ggccaagtat actgtctatc tatatttgtt
     1981 tcgttagtcg cgcaaaatct gcctggc
//
LOCUS       DAOQNZ010000800         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37769_length_2005_cov_3.980513, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000800 DAOQNZ010000000
VERSION     DAOQNZ010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37769_length_2005_cov_3.980513"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="PKW73_13505"
     CDS             <1..1757
                     /locus_tag="PKW73_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNA74355.1"
                     /translation="NNLLALDDVKYRLKSMQIKPVVCTEDDFQHFMETIYSTRQDELT
                     EEWEEQADQTFIESEVDLSSLNVLVDTDDAINDLDLARQAQDAPIVQLANQILAKAIK
                     RQCSDVHIEPQDKNLMVRYRLDGVLFVDRRLPKAILAALVSRFKIMADLDIAERRLPQ
                     DGRIRVRFSGKDIDFRVSTIPSKHGEKVVMRQLDKSGTTFGLNHLISDPDTLNIIREM
                     VSKPYGIIFVTGPTGSGKTTSLYSALAERNTPEVNILTAEDPIEYEMPGITQVQVLRE
                     KGLDFARILRSFLRQDPDIILVGETRDRETAKIAVEAALTGHLVFTTLHTNDAPGAVT
                     RLAEMEVDPYLVASATVGVIAQRLLRRVCADCKEDYRPERSTLEYLGLMDKNQERLAE
                     STIQYYRLRMDDEGMPIFTRGKGCESCNKTGYKNRIGVYEVMRMNDEIRDLVCKNATT
                     AMVRLAAKQSGMIPLKDYSLRLVGQGLTTADEVVRVTLSDTSGEEKLCGKCRNPISDE
                     FIKCPFCQHDLKVSCLRCGMLQQDGWQSCPKCGHSNIDTADEFCCHSCEAEIFGEWHT
                     CPYCQAERPAAGFNAVNN"
     gene            1810..>2005
                     /locus_tag="PKW73_13510"
     CDS             1810..>2005
                     /locus_tag="PKW73_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HNA74356.1"
                     /translation="MMSIEMQELLQLVVDRAASDLHLKAGEPPIIRAAGKLTRISLPI
                     LTDDDTKRLIFSCLSEEQRRF"
BASE COUNT          546 a          551 c          522 g          386 t
ORIGIN      
        1 ccaacaattt gctcgctctg gatgacgtca agtatcgact caaaagcatg cagatcaaac
       61 ctgtggtctg caccgaagac gacttccagc acttcatgga aaccatctat tccacaagac
      121 aggacgaact gacggaagaa tgggaggaac aggccgacca gaccttcatt gagtcggagg
      181 tagatctctc cagcttgaac gtgctggtgg acactgacga tgccatcaac gacctggatc
      241 tagccaggca ggcccaggac gcgccaattg tgcaactggc caaccagatc ttagcgaaag
      301 ccatcaaacg gcagtgctcc gacgtgcata tcgaacccca ggacaaaaac ctgatggtgc
      361 gctatcgcct cgacggcgtg ctattcgtag acagaagact gcccaaggcc attctggcgg
      421 cacttgtatc gcgcttcaaa attatggctg atctggacat cgccgagcgc cgcctgccgc
      481 aggacggccg catcagggtg aggttctccg gcaaagacat cgacttccgt gtttcgacca
      541 taccctctaa acacggcgaa aaagtggtga tgaggcaact ggataaatca ggcaccacct
      601 tcggcttgaa ccacttgatc tcagacccgg acaccctcaa catcatcagg gagatggtct
      661 caaagcctta tggcattatc ttcgtaaccg gaccgaccgg ctcgggtaaa accactagtt
      721 tgtactcggc cctggccgag cgcaacaccc cggaagtaaa cattctcacg gccgaagacc
      781 cgattgaata cgagatgccc ggcatcactc aggttcaggt tctgagagag aaaggtctgg
      841 attttgccag aatccttcgt tccttcttga ggcaagaccc ggacatcata ctggtgggag
      901 aaacccgcga tagagaaaca gccaaaatag cggtagaagc cgcacttacc ggtcacctgg
      961 tcttcaccac cctgcacacc aatgacgcac caggggcggt gacacgcctg gccgaaatgg
     1021 aagtggaccc atatctggtg gcatctgcaa cggtaggagt gatcgctcag cgacttttac
     1081 ggcgagtctg cgccgactgc aaagaagact atcgcccgga aagaagcacc ctcgagtatc
     1141 ttggactgat ggataagaat caggagcgcc tggccgaatc tacaattcaa tactaccgcc
     1201 tgcgcatgga cgacgagggc atgccgattt tcacccgcgg taaaggttgc gaaagttgca
     1261 ataagaccgg ctacaaaaac cgcatcggcg tctatgaagt gatgcgcatg aatgatgaaa
     1321 ttcgcgacct ggtatgcaaa aatgccacca cggccatggt tagactggct gctaaacagt
     1381 ccggaatgat ccccctgaaa gattacagct tgcgcctggt cggacagggt ctcactaccg
     1441 ccgacgaagt agtgcgcgtc accctctccg acacctcggg cgaagagaaa ctctgcggca
     1501 aatgccgcaa tcctatctcc gacgaattta tcaaatgccc cttctgccaa cacgatctaa
     1561 aagtgtcctg tctgcgctgc ggcatgttgc aacaagacgg ctggcaaagt tgccccaagt
     1621 gcggacattc caatatagac acagccgacg aattttgctg ccacagttgt gaggcggaaa
     1681 ttttcggcga atggcacact tgcccctact gccaggctga aagaccggca gccggcttca
     1741 atgccgtcaa taactgacag gcccggtaaa actcaacgga aaagaaacag gcgaaagaaa
     1801 agaaggtgaa tgatgtcgat tgagatgcag gaattgttgc aactcgtcgt ggacagagcg
     1861 gcaagcgatc tgcatttaaa ggcgggggag ccgccaatca tcagagcagc aggtaagctg
     1921 acacgaatca gtttgccgat tctcacagat gatgacacaa aacgcctgat tttctcctgc
     1981 ctcagtgaag agcaaaggcg ctttg
//
LOCUS       DAOQNZ010000801         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37775_length_2005_cov_3.627692, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000801 DAOQNZ010000000
VERSION     DAOQNZ010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37775_length_2005_cov_3.627692"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PKW73_13515"
     CDS             complement(<1..715)
                     /locus_tag="PKW73_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNA74357.1"
                     /translation="MTASVLVDGASGYLGLHLVQTLLQDGFQVRALVRPQSGQTDREQ
                     LEILGAEVFVAPLAEEVSAAEALILDRAFAGADFAVHLIGSIAPGRQESFGSLHPQAA
                     AVFAARAKQAAVKRAAIVTALGASQTSRSLYLHSKAEAEEVVCSLLGPEMVAIFRPSL
                     IIGRKVGRRDSKLVNRYRQLIRTRPLVPLINGGKNLLEPVFVGDLTQCITAALKLPPA
                     QGTGVFEIGGPNRVTMREFV"
     gene            complement(712..1017)
                     /locus_tag="PKW73_13520"
     CDS             complement(712..1017)
                     /locus_tag="PKW73_13520"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNA74358.1"
                     /translation="MYGLKIKGEGKGEALSGVKHFLVDYAHRHSHPLNALLHIIGVPL
                     AFFGLYKLLFQRGQNCVGTGAACLILGYWLQYMGHKKQGNEVGEVTLIRHLLRKIKR"
     gene            complement(1036..>2005)
                     /locus_tag="PKW73_13525"
     CDS             complement(1036..>2005)
                     /locus_tag="PKW73_13525"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA74359.1"
                     /translation="TGMVGRSVVSTLAQAGHSVLALARSPQPALFQGLPAVSFAAADL
                     TTVDPLELQSLCKGLDVLVHAAGFVDPLAGREEVFAVNLGGTRKIFEAATAAGVRQFI
                     HISSLSVITGQEDQFDLDESAPLRRCGEAYADSKVESEEFISKQNGPMVWTILRPGFI
                     YGEGERSWLPRVIASIRGGQAVLVDGGKRQTNVVYAGNLSRAVLLCLLNEKSYGQVFN
                     ITDGEPVTKKQLFDAVADACGCPRIKKDLPRWFLQPVCTLVTAFARGASAPVRAGLAR
                     FSPAAFRLVAVNQGFSIKKAQTLLGYPDKQCLPFAEAMQLTLKSFK"
BASE COUNT          499 a          580 c          519 g          407 t
ORIGIN      
        1 ccacgaactc tcgcatagtg acacgattgg ggccgccaat ctcaaatacg cccgttccct
       61 gggccggcgg cagtttcagg gcagccgtga tgcactgggt cagatccccg acaaagacag
      121 gctccagaag atttttgccg ccattgatga gcggcaccag gggtctggtt ctgatcaatt
      181 gcctgtagcg attgacgagt ttgctgtctc gacgaccaac ttttctgcca attattaaag
      241 agggtctgaa aattgcaacc atttcagggc ccaggagact gcatactact tcctccgctt
      301 cggccttgga atgcaggtaa agactcctgg aggtctggct ggcaccaaga gcggtgacaa
      361 tggccgctct cttgacggca gcttgtttag ccctggctgc aaagaccgcg gcagcctgcg
      421 gatgaaggct cccgaaactc tcctgccgac cgggagcaat agagccaatc aagtgcacgg
      481 caaagtccgc accggcgaag gctctatcca agatcagggc ttcggcggcg gaaacttcct
      541 ccgctagcgg agcgacaaaa acctcggcac ccagaatctc taattgttca cgatcggttt
      601 gaccggactg gggacgcacc agggctctta cctgaaatcc gtcctgcaga agagtctgta
      661 ccagatgcaa acccagataa ccactggcac catccaccaa aacggaagca gtcatctctt
      721 gatcttcctt agcaggtgtc ggatcaaagt cacctctccc acttcattgc cttgcttctt
      781 atgacccata tactgcagcc agtatccgag aatcaaacag gccgccccag tgccgacgca
      841 gttttgaccg cgctgaaaga gcagtttata aagtccgaaa aaggcaagcg gcacgccgat
      901 tatatgcaag agggcattta gcggatgtga gtgacgatga gcataatcca ccagaaaatg
      961 cttgactcca gagagcgctt cccctttacc ttcgcctttg atcttcaatc cgtacatggc
     1021 tctccttcac aaggtctatt tgaacgactt aagagttagc tgcatggcct cggcaaaggg
     1081 caaacattgc ttatccggat agccaagcaa ggtttgcgct ttcttgatgc tgaacccctg
     1141 attgacggcc accaggcgaa aagcagccgg ggagaagcga gccagaccgg cacgcaccgg
     1201 ggcggaggca ccgcgggcaa aggcagtgac aagggtacaa accggctgca agaaccagcg
     1261 aggcagatcc tttttgatgc gagggcagcc gcaagcgtcg gcaacggcat cgaaaagttg
     1321 tttcttggtt accggctctc cgtcggtgat attgaagacc tgaccataac ttttctcatt
     1381 caagaggcag agcaggacag ccctgctcaa attaccggca tagacaacat tggtttgcct
     1441 cttgccgcca tccacaagca cggcctgtcc acccctgatg gaagcaatta ccctgggcag
     1501 ccagcttctt tcaccttcac catagataaa accagggcgc agaatcgtcc agaccatagg
     1561 tccgttctgc ttactgataa attcctcgct ctccactttc gaatcggcat aggcttcgcc
     1621 gcaacgtcta agcggcgcac tctcatccag atcaaactga tcttcctgcc cggtgattac
     1681 agaaagagaa ctgatgtgaa tgaactgcct cacaccggcg gcggtggcag cctcaaagat
     1741 ctttctagta ccgcccagat ttacggcaaa gacctcttcc cttccggcca gaggatctac
     1801 gaaaccggcg gcatgcacca gaacatcgag gcccttgcat aggctttgca attccaaagg
     1861 gtcgacggtg gtgagatcag ccgcggcaaa gctaacggca ggcagccctt gaaagagagc
     1921 cggctgggga gaacgagcca gggccagaac cgaatgtccg gcttgagcca gggtcgacac
     1981 cacgcttcga ccgaccatac cagtg
//
LOCUS       DAOQNZ010000802         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37778_length_2005_cov_3.478974, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000802 DAOQNZ010000000
VERSION     DAOQNZ010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37778_length_2005_cov_3.478974"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /locus_tag="PKW73_13530"
     CDS             complement(<1..1995)
                     /locus_tag="PKW73_13530"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNA74360.1"
                     /translation="MTDQVAKTYITQTFVNDTDQNLAGTYLFPLPDDTTFSSFSLHID
                     GKPVEGKILEASEARQQYEAIVRQMIDPGLLEYADYKTVRARIFPIPAHGTKKVELEY
                     TQLLKAENGLVKYRFPLKGDREASAGPVDDTKMSVKLSGKQGLRTIWSPSHTVQVDRP
                     ENNRAKIAYHQANALQDKDFLLYYSLSDKEMTANLLTHKVDGEDGYFLLSLAPPLKAA
                     QSVAKDVVLVADTSGSMQGEKLEQSKKALKYVVTALSPQDRFGIVPFNTDADSFRGQL
                     VQATTDNKRLAVDFIDELEARGGTNIGEALRTGGSLLKDGGDRPAYVVLMTDGEPTVG
                     ETSLTGLLKTVSNKNIRLFDFGVGYDVNTRLLNRLSSDHHGTSQYIEPGESLEVAMSG
                     FYDKIRSPMLSDVQLTFSGITVKDIYPRQVKDIFAGNQVLLLGRYKGSGQASVNISGK
                     LAGAAKTFSFPINFAGAEASNSHLSRLWAMRRIGYLTEVAQENGDTREVIDEIVALSK
                     KHGIITNYTSFLVTDPSENHRLANNRPMPAASPVANRENSRWFAQPRRIAVSDESPRM
                     TDWRQVRLQSKAAGAPGAGGRAQFAAAMPLPSQGEESMGRAVGPAAERDSKDKLRQSL
                     AASSGKLAFDREKKASALKEVSQMDGFSGNAAESKQIRSVE"
BASE COUNT          416 a          551 c          547 g          491 t
ORIGIN      
        1 ttccacgctt ctgatttgct tgctttccgc tgcattgccg gaaaatccgt ccatctggct
       61 cacttccttc aaagcactgg ccttcttctc tctatcgaaa gccaatttac cagaggaggc
      121 cgccagagac tgacgcaact tgtccttgct gtcgcgctca gccgctggac caacggcccg
      181 gcccatactt tcttccccct ggctcggcaa tggcattgcc gcggcaaact gggctctacc
      241 gccggcgccg ggcgcaccgg cggctttact ttgcagtctc acctggcgcc agtcggtcat
      301 tctgggcgac tcgtcagaaa cggcaattct gcggggttgg gcaaaccaac gactgttctc
      361 acggttggca actggagatg ccgccggcat cggtcggttg ttggcgagac gatgattctc
      421 gctggggtcg gtgaccagga aggatgtgta attagtgatg atgccgtgct tcttagacag
      481 tgccactatc tcatcaatga cctcccgggt gtcgccgttt tcctgggcca cttccgtcaa
      541 ataaccgatg cggcgcatgg cccagagcct tgaaagatga ctgttggagg cttcggcgcc
      601 ggcaaaattg atggggaaac tgaaggtctt ggccgccccc gccagtttgc cggaaatgtt
      661 gacgctggct tgtccggaac ctttgtagcg gcctaataaa agcacctggt tgccggcgaa
      721 aatatctttc acttgcctgg ggtagatgtc tttgacggtt atgccgctga aagtcaactg
      781 tacatccgag agcatgggcg aacgaatttt atcgtaaaag ccagacatag ccacttccag
      841 agactcaccg ggctcgatgt attgcgaagt accgtggtga tcggaagata gccggttcaa
      901 aagcctggtg ttgacgtcgt aaccgacgcc gaaatcaaag agcctgatgt tcttattgga
      961 gaccgtcttc agaaggccag tgagggaagt ctcgcccacc gttggctctc catcggtcat
     1021 cagcaccaca taagccggtc tgtcgccgcc gtccttgagc aaactaccgc cggtgcgcag
     1081 ggcttccccg atattggtgc cgccccttgc ctccagctca tcaatgaagt ctacggccag
     1141 gcgtttgttg tcggtagtag cttgcacaag ctgaccgcga aaggagtcgg catcagtatt
     1201 gaagggcact ataccgaagc gatcctgggg cgagagggcc gtcactacat atttaagggc
     1261 tttcttgctc tgctccaatt tctcaccctg catggaaccg gaggtatcag ccaccagaac
     1321 cacgtctttg gcaacgctct gagctgcctt caaaggtggt gccaggctga gcaagaaata
     1381 accatcttca ccgtccacct tatgagtgag caaattggcc gtcatttcct tatcggaaag
     1441 gctgtaatag agcaggaagt ctttgtcttg aagggcatta gcttgatggt aggcgatctt
     1501 ggcccgatta ttctccggtc tgtccacctg caccgtatgt gatggcgacc agattgtgcg
     1561 caggccctgc ttgcctgaaa gcttgacact catttttgta tcatcgaccg ggccggcact
     1621 ggcttcccgg tctcctttca ggggaaagcg atatttgacc agtccgtttt cagctttcag
     1681 aagctgggtg tactccagct caaccttctt cgtaccatgg gcgggaatag ggaaaatgcg
     1741 cgcccgcacc gtcttgtagt cggcgtattc cagaagaccc gggtcaatca tctgcctgac
     1801 tatggcttca tactgttgcc tggcttcgct ggcttccagt attttgccct ctaccggttt
     1861 accgtcgatg tgcagggaga aactggaaaa ggtggtgtcg tccggcaggg gaaacaagta
     1921 agtaccggcc agattctgat cggtatcgtt gacgaaagtc tgggtgatat aagtcttcgc
     1981 cacctggtcg gtaatctcaa cctta
//
LOCUS       DAOQNZ010000803         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37837_length_2003_cov_3.808008, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000803 DAOQNZ010000000
VERSION     DAOQNZ010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37837_length_2003_cov_3.808008"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="PKW73_13535"
     CDS             <1..1646
                     /locus_tag="PKW73_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74361.1"
                     /translation="TGALNAQILIRDGKLSKQQAVEALKEARQRQVTVEVQLKEKGFY
                     DLPSRSCPRLGELLLFCGVISQSDLVSALELGLMNKTPVGQVLTSSNQVSRKIMDAAL
                     HVQNLMAEQTMSITDARAVISAVKEGSTLEDAISELQTEAEESGSEEAEPEKHYLSLF
                     EFLKNLGRTDEAQVNEAFQIAMHNSDVLSQVLLISGSLDAKTIERAEKCRKLVQDKKL
                     PMEHGNIAFDYAERFDIDVSQALKELQWHRPEEMDEEELEALTEESAERPAVAEKSGV
                     GAAKAAAHKGGHAEKTPAGRGRGVTGGGGNGADKEHEDGAEADGSEAVDAHAEAVSEW
                     ETLTKKLEHLSVSGKLDKALDTRIRLLQLAEEFFPERRIDCLDAVAALYVQRLNMDQA
                     EQYYLKSMELRKAEGEDNGPRLAQGYCNLGKVSYFKQDYPAAEEHARKFIEVIANSLG
                     KGHPDVACGWQNLANIFYVQKKYVQAQRAYQVGIQICEKGLGETHPTTVQMKRSYSIL
                     LAAMDPDDAANLRRSGSLGVITGSWRTLPPDAGQSLHEK"
     gene            1794..>2003
                     /locus_tag="PKW73_13540"
     CDS             1794..>2003
                     /locus_tag="PKW73_13540"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HNA74362.1"
                     /translation="MVSTGASRADTGDDRPPVVLELFTSEGCSSCPPADKLLAELSKM
                     NGSSVIILSEHVDYWDYLGWRDPFSA"
BASE COUNT          485 a          481 c          597 g          440 t
ORIGIN      
        1 tgaccggtgc tctcaatgct cagattctca ttcgcgacgg caagctgagc aaacagcagg
       61 ccgtagaggc tttgaaggaa gccaggcagc gccaggtgac agtggaagtg cagttgaagg
      121 agaagggctt ctatgatttg cccagccgca gttgtccgcg actgggagag cttttgcttt
      181 tctgtggagt tatttctcaa tccgacttag tcagtgctct tgagcttggc ttgatgaata
      241 agactccggt ggggcaggtt ctcacctctt ccaaccaggt cagccgcaag attatggatg
      301 ccgccctgca cgtgcaaaac ctcatggccg agcagaccat gagtatcacc gatgcgcgtg
      361 ccgtgatcag tgccgtgaag gaaggcagta ctctggagga tgccatttcc gagcttcaga
      421 ctgaggcgga ggaatcgggt agtgaggaag cggaaccaga gaagcattac ctgtcgctat
      481 ttgagttcct caagaacctc ggtcgcaccg atgaagcgca ggtgaacgaa gctttccaga
      541 tcgccatgca caattccgac gtgctttcac aagtgctttt gatttcaggc agccttgacg
      601 ccaagaccat cgaaagagcg gagaagtgcc gcaagctggt gcaagacaag aagttgccca
      661 tggaacacgg caatatcgct tttgactatg cggaaaggtt cgatatcgac gtttcgcagg
      721 ctcttaaaga gttgcagtgg cacagaccgg aagagatgga cgaagaagaa ctggaggccc
      781 tcaccgagga gtctgcggaa aggcccgctg tggcggagaa gtcgggagtt ggtgcggcaa
      841 aagctgccgc ccacaaaggt ggtcatgcgg aaaaaacgcc ggcaggtcgg gggcgtggtg
      901 tcaccggtgg tggtggcaat ggtgccgaca aagagcatga agacggtgcc gaagctgatg
      961 gctcggaagc tgtggatgcc catgccgagg ctgtaagcga gtgggaaact ttaaccaaga
     1021 agcttgaaca tctgagtgtc tccggcaagc ttgataaagc tctcgatacg cgcatcaggc
     1081 ttttgcaact ggcagaagaa ttcttccccg agcgtcgtat cgattgtctc gatgccgtag
     1141 ccgcccttta tgtgcagcgt ctgaacatgg accaggccga gcagtattat cttaaatcta
     1201 tggaactgcg caaggcggag ggtgaggata atggccccag gctggcccaa ggctattgta
     1261 accttggcaa agtaagctat ttcaaacagg attacccggc ggcagaagag catgctcgca
     1321 agtttatcga agtaattgcc aacagtctcg gtaaaggaca ccctgatgta gcctgcggct
     1381 ggcagaatct ggctaatatt ttctatgtgc aaaagaaata tgtgcaagct cagcgtgcct
     1441 atcaagtagg tattcaaatc tgtgaaaagg ggctgggaga gacacatccg accacggtgc
     1501 agatgaagcg cagctacagc attttgcttg ccgccatgga tcccgacgac gcggccaact
     1561 tgcgccggag cggctccctc ggcgttatca ccggcagttg gcgcaccctg ccgccggatg
     1621 ccggtcagtc tttgcacgag aagtaagtca ctgtctctga aaactttctg gttgactgcg
     1681 ggaataaacc cctgctctcg ctcgtatatg aacgtgtaag cgtgattgga gtggggaagt
     1741 caggaagaat gagattagtt ccaggaatat tgtttctagc agccctcttg gcgatggttt
     1801 caacaggagc ctccagggct gatactggag acgatagacc gccggtggtg ctggaattat
     1861 tcacctcaga gggttgcagt agttgtccgc cggcagataa gctgcttgcg gaactttcca
     1921 agatgaacgg ttcctcggtc atcattttga gtgagcacgt agattactgg gactatcttg
     1981 gctggcgcga ccccttttcc gct
//
LOCUS       DAOQNZ010000804         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37875_length_2002_cov_4.563945, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000804 DAOQNZ010000000
VERSION     DAOQNZ010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37875_length_2002_cov_4.563945"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1019
                     /locus_tag="PKW73_13545"
     CDS             279..1019
                     /locus_tag="PKW73_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74363.1"
                     /translation="MPEEEDVEELENIEDMMPEDFLDLLSATPPTELIAKYPRPELAV
                     AAEGAAELFYSDEETDKKKFARLLDLTNRLCKSKEKANGLNLANSYYCLASFVYRAKE
                     YEEAADLALKAVTTRLNSGSEIAESSALHDYFLLVNALCWAKQFEEAHKQASAWLTLA
                     DKVHTQGDTYKAEIHLLLARIYEELSKIAEAEQEFTKALEVHDKAKSKNPGEAKNAYH
                     AFGEFLKRRGRDGEAEEFLTKAKAIKAK"
     gene            1038..1760
                     /locus_tag="PKW73_13550"
     CDS             1038..1760
                     /locus_tag="PKW73_13550"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNA74364.1"
                     /translation="MCGRFTLSCDQAELIERFQVDDVFMETALTPRFNIAPSQSVAAI
                     VALSKDGKRVLTSFQWGLIPFFVKEPAKARPMINARAETLAEKPFFKTALLRRRCLIP
                     ADGFYEWRTVPGTKKKQPLWIHLKGESSERAPLFAFAGLYDKNEHILNAAGEPVKTCT
                     IITTEANESIKPVHDRMPVILGAEGIKLWLDPSVQDPAQLSAILQPLPANVITMFPVS
                     TRVNSPSYDGKDLILPDPTESQ"
BASE COUNT          550 a          557 c          501 g          394 t
ORIGIN      
        1 acgggcacag gcgatgccag cccgcaagaa aaagggcgat tgaccgtagt cattttattg
       61 gagctcctgg atagtaaagg gaccttttcc cagtcaacgt tgccaagata ataagaccgg
      121 tacccacact ttgacaccgg aaaaacccta gaccgaaaac cgccaaaaac accggcagtg
      181 gcaaaggaaa gtcaaaaatt cttatggtgc cggccgggtc tggccaaaat cgcacttcgt
      241 ctggtatata tagacggtct acccagggga cttggaatat gccggaagaa gaagacgtcg
      301 aagaactcga aaacattgaa gatatgatgc ccgaggattt cctcgatctt ctcagcgcca
      361 cgccgcccac cgaacttata gccaagtatc cgcgtcccga actggctgta gctgccgaag
      421 gggccgccga actgttctat tccgacgaag aaacagacaa aaagaaattc gccagactgc
      481 tggatttgac aaaccgcctt tgcaagagca aggaaaaagc aaacggtctg aacctggcca
      541 attcatacta ttgcctcgcc tccttcgtct acagagcaaa agagtatgaa gaagccgccg
      601 atctggctct gaaagccgta accacacgct tgaacagcgg cagcgaaatt gcggaatctt
      661 ccgctctgca cgattatttc ttactggtaa acgccctttg ctgggccaaa cagttcgagg
      721 aagcccataa gcaagcttcc gcctggctga ccctggccga caaagtgcac acccagggag
      781 acacttacaa agctgagatt catcttttgc tcgcccgcat ttacgaagag ctgagtaaaa
      841 ttgccgaagc cgagcaagaa ttcactaagg cactggaagt acacgacaaa gccaagtcga
      901 aaaatcccgg cgaagccaaa aacgcttatc atgccttcgg cgaatttctc aaacgccgcg
      961 gcagagatgg agaagcggaa gaatttctca ccaaagccaa ggccatcaaa gcgaaatagg
     1021 gcgccgacac agagataatg tgcggtagat tcacacttag ctgcgatcaa gctgagctta
     1081 tcgagcgttt tcaggtagac gacgtcttca tggaaacggc tctgacgcct cgcttcaaca
     1141 tcgcaccatc gcaatcagtg gccgctattg tggccctctc caaagatggt aagcgggtcc
     1201 ttaccagctt tcagtggggt ctaattccgt tctttgtgaa agagccggcc aaggctcgcc
     1261 ccatgatcaa tgccagagcg gagacactgg ctgaaaagcc gttcttcaag acagccctgc
     1321 taaggcggcg ctgcctgata ccggccgatg gtttctatga atggcgcacg gtgccgggca
     1381 ccaagaagaa gcagccgctc tggatccacc tcaagggcga aagcagtgaa agggctccgc
     1441 tatttgcttt cgccggactc tatgacaaga acgagcatat tctcaatgcc gccggtgagc
     1501 cggtaaagac ttgcacgatc atcaccacgg aagcaaacga aagtataaag ccggtgcacg
     1561 accgcatgcc cgtcattctg ggagcagagg gcatcaagct ctggctcgat ccttccgtac
     1621 aagacccggc tcaactctcc gccattctgc aaccgctgcc ggccaacgtc atcaccatgt
     1681 ttcccgtatc caccagggtc aattcgcctt cttatgacgg taaagattta attctgccgg
     1741 atccgaccga aagccagtag cctggcgatg cggccctcat ctgctcgtta acagtcggga
     1801 ttagccgagc tttcagccgg gtaactgact aattgaatgc tagcggcaat atacgaggac
     1861 aatcatgaaa ttaaagaccg cgagacgctg tctcattctg gctacggtca tgctcggtca
     1921 aatcagccag cctggcttct gcaagccttc ggccggtgat gacggtgagg gccagaccca
     1981 caaacacggt aaagccacca cc
//
LOCUS       DAOQNZ010000805         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37898_length_2002_cov_3.015922, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000805 DAOQNZ010000000
VERSION     DAOQNZ010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37898_length_2002_cov_3.015922"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKW73_13555"
     CDS             <1..377
                     /locus_tag="PKW73_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74365.1"
                     /translation="MLARCDKYSQARQVYFREGENLSPLKSLGGVQIEKSELYLTLHY
                     RGQSMHVEEPGKIPVTWLNPDVQYVLTRATTVSLSPLVASAGQPFAVPGLTAPVSLKL
                     VNNVQWENGGIDPFTARYYVNE"
     gene            370..990
                     /locus_tag="PKW73_13560"
     CDS             370..990
                     /locus_tag="PKW73_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74366.1"
                     /translation="MNKTRRLFVNSKLAVLILIMPLFLLVSSMYPALGFKLFDQSETV
                     VDLKVKAAKNLFDYKGTVLGEPVKASVFFTDKDGGKCHYQDLWFTGATAIGLNRFSAL
                     SVPSGSSVAIAIAMEDAGSATQVQAAANASLRTLLELYVEGHSVSRLEVADDDTARAL
                     ISSLGQAGFVESAAVLPDELVETSLSLSVKSRSSNRLYSLNYGVTQ"
     gene            990..1907
                     /locus_tag="PKW73_13565"
     CDS             990..1907
                     /locus_tag="PKW73_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74367.1"
                     /translation="MSDSSVSYRSKRGSVLLLSFLMGWLVVVILLAIVTVCLLCVSAA
                     KSRSLAEEKSLAMAACLNKGNRLGELNHMIGYSRQLVFSSRQVNNEVLSKYPQLKALS
                     GYLMDDARSGACKVKEEFEIVRDMSFRDVRTLQESCDKSFQGAAFSLPGLEMESPRVE
                     AITLGSLAGMPSNASLPAALEELREHDERSGYKAGGSSFYRGSIDARLPLPDNDLSFK
                     FSCLPPCAPGQAQVEQLRLVRFESLESIAKMRSQCSFRREGDKGFAPCAAQLDLGAWV
                     TLAPGCLQHVGFYQQQAASCSSGDSAAGL"
BASE COUNT          411 a          499 c          562 g          530 t
ORIGIN      
        1 gcatgttggc tcgctgcgat aagtattctc aagcacggca ggtttatttc agggagggtg
       61 agaatctctc cccattgaag agcctgggcg gggtacagat tgaaaaatcc gaactctatt
      121 tgactcttca ttatcgtgga caatcaatgc acgtggaaga gccgggaaag attccggtta
      181 cttggctcaa ccctgatgtg caatacgtct taaccagagc taccacggtc agcctctcgc
      241 ctctggtggc ttccgccggt cagccctttg cggttccggg tttgactgca ccggtttctt
      301 tgaagctggt caacaatgtg cagtgggaaa acggcggcat agatccgttt acggcgagat
      361 attatgtcaa tgaataaaac gagacgacta tttgttaaca gtaaattggc ggtgctgatt
      421 ctaatcatgc ccttgtttct gctggtttca tccatgtacc cggctctggg atttaagtta
      481 ttcgatcagt cggagacggt tgtcgatctg aaagtcaaag ccgctaagaa tttgttcgac
      541 tataagggca ccgtcctcgg tgagcccgtc aaagcttccg tattcttcac ggataaggac
      601 ggtggcaaat gtcactatca ggatctctgg tttaccggcg ctactgccat cggcttgaac
      661 cgattctcgg cgctgtcggt tccttctgga tcttcggtgg caattgctat tgcaatggag
      721 gatgccggct cggctacgca ggtgcaggct gccgccaatg cctctttgcg aacactactt
      781 gaattgtatg tagaggggca ctcggtttcc cgtctggaag tggccgatga cgatacggcg
      841 cgggccctga taagcagtct ggggcaggcc ggttttgtag agagtgccgc cgtcttgcct
      901 gatgaattgg ttgagacttc tctctctctt tcggtcaaaa gccgcagttc caatcgcctt
      961 tactccctca attatggagt cacgcagtaa tgtctgattc atccgtcagc tacaggtcga
     1021 agaggggctc ggtactgctc ttgagctttt tgatgggctg gttggtggtg gttattttgc
     1081 ttgccatagt gaccgtttgc ttgctctgtg tttctgctgc taagtcacgt tcgctggccg
     1141 aggaaaagtc tctggccatg gccgcttgcc tgaacaaagg taaccggctt ggggagctta
     1201 atcatatgat tggttatagc cgccaactgg tattttcttc gcgacaggtc aacaacgagg
     1261 ttttgagtaa gtacccgcag ctcaaggctc tctcaggcta cttgatggat gatgcccgta
     1321 gcggtgcctg caaggtgaaa gaggagttcg aaattgtacg ggatatgtct tttcgcgatg
     1381 tgagaacact gcaagagtct tgtgacaagt ccttccaggg tgcggctttc agcctgcccg
     1441 gtttggagat ggaaagcccc cgtgtagagg ctatcaccct cgggtcactg gccggaatgc
     1501 ccagtaatgc cagcctccct gcggccctgg aggaactgcg agaacatgat gaaagaagcg
     1561 gttacaaagc aggagggagc agcttctatc gaggtagcat cgatgcgcgt ctgccgctgc
     1621 ccgacaatga tcttagtttc aagttctctt gcttaccgcc ttgcgctccc gggcaggcgc
     1681 aagtggaaca attgcgtctg gtgcgcttcg agtctctgga gagcattgct aaaatgcgca
     1741 gtcagtgttc ctttcgccgg gagggtgata agggctttgc gccctgcgcc gctcagcttg
     1801 atcttggcgc ctgggttacc cttgcccccg gatgccttca gcacgtgggc ttctatcagc
     1861 agcaagcggc atcctgtagc agtggagatt cggcggccgg gctttaaaaa tagtttgcaa
     1921 gccactttta gtcttcaact tctatggcca gatcaaacca gaaggtactt ccccgtccgg
     1981 cctggctctc gaccccgatg tt
//
LOCUS       DAOQNZ010000806         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_37960_length_2000_cov_4.124422, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000806 DAOQNZ010000000
VERSION     DAOQNZ010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37960_length_2000_cov_4.124422"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKW73_13570"
     CDS             <1..636
                     /locus_tag="PKW73_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74368.1"
                     /translation="FDQKEGGIWLPLLEKAYGTWRNSNQAPYYVVLRTAKHLIFEGRF
                     SPQTELPGFGASFGAADNGATEALTGHGMKEYSTFHFDCGDFGLGKGYVTLRQMESWL
                     GREAVRKKYIDEQDKALTEAIRNQLICIATTEISGNCQTYGLRSGHAYAVIGYDPKIR
                     CIRLADPYGAGDFKLLSTGQPIDKVDDGIFQLSLHDFNELFSHLRIEEIPN"
     gene            complement(633..1052)
                     /locus_tag="PKW73_13575"
     CDS             complement(633..1052)
                     /locus_tag="PKW73_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74369.1"
                     /translation="MSGDRGDNVRKAVYDPNQDSAGDNLRQVAFNPQECGKFQEMLPH
                     MQTSNLFQRMDQFVGGTMRQNGLDMKQLDGMLQSDKFNDKDKKVFQYMKDNFAQAAPV
                     LGNPERLSMGELQNHLAKAVFQACDQRGAPGQQRSKR"
     gene            1240..1911
                     /locus_tag="PKW73_13580"
     CDS             1240..1911
                     /locus_tag="PKW73_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA74370.1"
                     /translation="MSKILLIEDDQELSTTVAKWLTLERHAVEVTGDGLNGLELILGG
                     DFDIIVCDVNLPGMDGFQICRRYRDAGGQTPIILLTGKAQIQDKETGFASGADDYLTK
                     PFSVRELCARIRALLRRPDTYRGSVTVGKLHLDLTARKILKDGKPLSLLPVDYALLEF
                     LMRHPGETFSTDSLISRVWATDKSPTVEAVRSSVKRIRKVIDEPGLESMIETVNKIGY
                     RLNKN"
BASE COUNT          499 a          555 c          508 g          438 t
ORIGIN      
        1 tttgatcaaa aggagggtgg aatctggcta ccgcttctgg aaaaggctta cgggacctgg
       61 cgaaacagta atcaggcgcc ttactatgta gtccttcgca cagccaagca cctgatattc
      121 gaaggccgct tcagtcctca aacagaattg cccggcttcg gcgcttcctt tggcgcggcc
      181 gacaacggag caacagaagc cctcaccgga cacggaatga aggagtactc gacttttcat
      241 ttcgactgcg gcgatttcgg tctcggtaaa ggctatgtca ctctcaggca gatggagagc
      301 tggctggggc gcgaagccgt acggaagaaa tatatagacg agcaagacaa agctctcacc
      361 gaggccatca ggaatcagct aatctgcatc gccacaacgg aaatttccgg caactgccag
      421 acttacggac tacgttccgg acacgcctat gccgtcattg gctacgaccc caaaatccgc
      481 tgcatcaggc ttgccgaccc ctatggtgcc ggtgatttca aactgctcag cacagggcag
      541 cccatagaca aagttgacga tggcatattt cagctttcgc tgcacgactt caacgagcta
      601 ttctctcacc tgcgcataga ggaaattccg aactagcgct tagaccgctg ttgtcctgga
      661 gcgccgcgct ggtcgcaggc ctggaacaca gccttagcta gatgattctg taattctccc
      721 atagaaagac gctcgggatt cccaagcacc ggtgcggcct gggcgaagtt atccttcata
      781 tactgaaaga cctttttgtc tttatcgtta aacttgtcac tctggagcat gccatccagt
      841 tgcttcatgt caagtccgtt ctgacgcatg gtgccgccga caaactgatc cattcgctgg
      901 aacaggttgc ttgtctgcat gtgtggcaac atttcctgga acttgccgca ctcctgggga
      961 ttaaaggcga cctgcctgag attatcaccg gcactatctt gatttggatc gtacactgct
     1021 ttgcgaacgt tatcgcctct atctccagac atagctaagc ccccaaaatc taacgtctca
     1081 ctctctatac ccagtgaaag cggtaggaaa acccaccgcc gccgggcacg acccaaaccc
     1141 ggaaggacgg ggggagggat ttttggactg gaatgaatgg aagctttgca actagcggac
     1201 tgcgcggtta gcattttcgg atgctaatat catatgccta tgtcaaaaat actcctgata
     1261 gaagatgacc aagagctttc caccaccgtg gcgaagtggc tcaccctgga gcggcatgcc
     1321 gtagaggtga ccggcgacgg tctcaatgga ctcgaactga ttctgggcgg cgattttgac
     1381 atcattgtct gcgatgtcaa tctacctggc atggacggtt tccagatctg cagacggtac
     1441 cgagacgccg gcggtcagac accgatcatt ttgctcaccg gtaaagccca aatccaggat
     1501 aaggaaaccg gcttcgcctc cggggcagac gactatctca ccaaaccatt tagcgtcaga
     1561 gagctgtgtg cccgcatacg tgcgctcttg cgcagaccgg acacctacag aggtagtgtc
     1621 accgtgggta agctgcatct ggatctcacg gccaggaaaa tcctcaaaga tggcaaaccg
     1681 ctctctctcc tgccggtgga ctatgcacta ttggaatttc tcatgcgcca tcccggtgaa
     1741 actttcagca cagacagcct catttcaaga gtctgggcca ccgataagag ccctaccgtg
     1801 gaggctgtgc gctcttccgt caaacgcatt cgcaaagtca tagacgaacc gggtctggaa
     1861 tctatgatcg aaacagtaaa caagataggc tatcgcctca acaagaatta gtgccgggtg
     1921 aatttgaccg atatcggcaa aacccaagac ccgagagcat gcagacccaa gcgcttggca
     1981 acagacatgg ccggcaccat
//
LOCUS       DAOQNZ010000807         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38044_length_1998_cov_3.500772, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000807 DAOQNZ010000000
VERSION     DAOQNZ010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38044_length_1998_cov_3.500772"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKW73_13585"
     CDS             complement(<1..461)
                     /locus_tag="PKW73_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74371.1"
                     /translation="MPTIATAPVLLTPVSDEDLGQLEIALPNVSVISYAGPGGTGGLP
                     ASLSPVVKRLNTRVNWFALTDLPEQDFSPTGSSGFTFHNPNVPQWLIKAHRRFCQNYL
                     APLLHGFPEKAVFDQEDWKSFKQLNESIASHCLIANSESYPSLFWLHDYQLA"
     gene            complement(723..1250)
                     /gene="rimM"
                     /locus_tag="PKW73_13590"
     CDS             complement(723..1250)
                     /gene="rimM"
                     /locus_tag="PKW73_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNA74372.1"
                     /translation="MDADDFTSKIAELSGLKGLRGDFKLTLFTGFHLIDRIKKVKLVT
                     DSATTYATVSKIYPEGRAILLRLKEYPDRTAAETMVGATVFTQRCQMGGLTEDEFWIN
                     DLIGMEAYTTGGTKIGTISAVYGEGNQLLEVTANDTTPETEKTHLVPFVKALVPKVDV
                     RARRVEIVDIPGLLE"
     gene            complement(1254..1910)
                     /locus_tag="PKW73_13595"
     CDS             complement(1254..1910)
                     /locus_tag="PKW73_13595"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF004685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNA74373.1"
                     /translation="MENENGKSKLPFVVAITGASGAIYGLRLLQFLLSSGQPVDLLLS
                     KAAQRVMKEEHDLILEGDVESLLLSYLKLPKAVPLKLHPLNDYGASVASGSYRTRGMV
                     VLPCSLGTLGALAAGLTENLIHRAAAVTLKERRKLMIVVREMPFGHIQLKNMLALSEA
                     GAIVSCASPGFYHRPETVQDQVDFVVGRVLDQFDFDNQLFKRWKEDSRPIVQEKKVGQ
                     "
BASE COUNT          440 a          474 c          550 g          534 t
ORIGIN      
        1 gctagttggt agtcgtgcaa ccagaacagg ctggggtagc tctcggagtt tgcgatcagg
       61 cagtgagagg caatcgattc attcagttgt ttgaatgatt tccagtcttc ctgatcaaag
      121 acggcctttt ctggaaaacc gtgcagaagc ggcgccaggt aattttggca gaagcgacgg
      181 tgggctttga tcaaccactg gggcacattg ggattgtgga aggtgaatcc gctcgaaccg
      241 gtgggcgaga aatcttgttc aggtaaatct gttagtgcaa accagttgac cctggtgttg
      301 agtcgtttta ctactggcga aagtgatgca ggcaagccgc ctgtgccgcc cggtccggcg
      361 taggaaatga cgctgacatt ggggagggcg atttccaatt ggccgaggtc ttcatcggac
      421 accggcgtca aaagaacggg agctgtggca attgtgggca tggtgaaagt ctcggacgaa
      481 aagtactcaa cgcgagatac cagaccggtt taaagctata ccggcctact taatttgccg
      541 cccattcaag agggcttttt aaaagggagc accgggggtg ctttgctccc aatttatttc
      601 aatttcataa cttggtaata aggtagtgga tatgcttgag gatatcaagt tgccatgtgg
      661 tgatttaatg ggagttattg ttaatgttct gttgcggata tcccccatat gggggcttcc
      721 gctcattcca gcaggccggg aatgtctact atttccacac gtcgagcacg aacatcgacc
      781 ttcggcacca gggcttttac aaaaggcacc aaatgtgttt tctctgtctc gggagtggta
      841 tcgttggcgg tgacttccag tagctggttg ccttccccgt aaacagcgct gatagtgccg
      901 attttcgtgc cgccggttgt gtaggcttcc atgccgatta agtcgtttat ccagaactcg
      961 tcttcggtca gtcctcccat ttggcagcgc tgagtgaaga ccgtggcgcc taccattgtc
     1021 tctgccgcag tgcggtccgg atattctttc aggcgcaaaa gaattgcccg tccttccgga
     1081 tagatcttgc tgacagtggc gtatgtggtg gcactgtctg ttactagttt tactttcttg
     1141 attctgtcta tcaagtgaaa acctgtgaac agagttagtt tgaagtcgcc tctcaagccc
     1201 tttagtccgg aaagctccgc gatttttgag gtgaagtcgt ctgcgtccat tgtctattga
     1261 ccaaccttct tctcttgcac tatggggcgg ctgtcttctt tccatctttt gaatagttgg
     1321 ttgtcgaaat cgaattgatc gagcactcgg ccgactacaa agtctacttg atcctggacg
     1381 gtctcagggc gatgatagaa ccctggagaa gcacaggaaa cgatggcacc ggcttccgac
     1441 aaggccagca tatttttcaa ttgaatgtga ccgaagggca tttctcttac tacgatcatg
     1501 agcttgcgac gttctttcag cgtgacggcg gcggctctat gaatcaagtt ttcagtcagt
     1561 ccggcggcaa gcgcaccgag ggtccccagg ctgcaaggca gcacgaccat accgcgggtt
     1621 cggtaggaac cgctggctac actggcacca tagtcgttga gagggtgtaa ttttagtggt
     1681 actgcctttg gtagcttcaa ataggaaagc aggaggcttt cgacatcccc ctctaagatc
     1741 aggtcgtgct cttctttcat gacacgctgt gcggccttgg aaagaagtaa gtcgacaggc
     1801 tgtccggacg atagcagaaa ttgcagcagc cgcagtccat agatggcgcc gctggctccc
     1861 gtgatagcca ccacgaaagg aagtttcgat ttgccgtttt cgttttccat tgcttttctt
     1921 gcccgaggcc tcgttactgc cacctataag gctagcatct tgccgggggc ttgcacctaa
     1981 aagatgtctt ggaaaagg
//
LOCUS       DAOQNZ010000808         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38138_length_1996_cov_2.285935, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000808 DAOQNZ010000000
VERSION     DAOQNZ010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38138_length_1996_cov_2.285935"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PKW73_13600"
     CDS             <1..625
                     /locus_tag="PKW73_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA74374.1"
                     /translation="SLPFAEHLERPQAEVQAIRAKTGRTFIGVELKVVRPDGTCVEKN
                     SMDVGEIIARGPNVFRGYWHNEEATREALRDGWIYTGDLAVVDADGYVNIVDRKKDMI
                     ISGGENIYSTEVEHALHDHPQIIECAVFGVPDEKWGESIKACVVTKAGAVLKEEQVIE
                     FVKERLAHYKAPREVEFMDELPKTGSGKILKKVLRDRCWQGRVKRVN"
     gene            740..>1996
                     /locus_tag="PKW73_13605"
     CDS             740..>1996
                     /locus_tag="PKW73_13605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74375.1"
                     /translation="MTKQNIATIGSTRKPLTCLKAKVIYALIVLSLYTTCGALAAPTA
                     ESQTSMQQFKVAKAAYMQGEYELAKKHLLAAVDSDKSNWEAHYLLGLVLGQEGNFSLS
                     IAHAQKSLAVNPDFAPAYSVLGRSLAEAQKLPEARQALETACRLDPKSAGNQCNLGAV
                     LGMSKDFKGAYAAYKKAAELDPKYVNAYLGMSSCLGKQGDKKGQIEACRKACEMVPGS
                     ALAHAKLGLLLSENGDIGGSMSEGYTANVLRLKESWNDFLGTFLTAWASVFLVFAGIF
                     AVIFAGSKFKPQEGEAVLRSFFLTFYKDKPGRFVVTSDRLVFVPEAFSAWFGSTNVSI
                     QRGQIESINYLSTVGGGTVSILTRDGSVHQFRMPLLVLDPLRSLLVGQGIVSKEPSAE
                     APAAATAEEKKEEKKEEPAAPAKTEET"
BASE COUNT          485 a          477 c          550 g          484 t
ORIGIN      
        1 cagtttgcct tttgccgagc atctagagcg tccgcaagct gaagtgcagg caatacgggc
       61 caagaccggt cgcaccttta tcggtgtcga actgaaagtc gtgcgtccgg acggcacctg
      121 cgtagaaaag aacagtatgg atgtggggga gattattgct cgcggtccaa atgtttttcg
      181 cggctactgg cacaatgaag aagctaccag agaggctctc cgcgatggtt ggatttatac
      241 cggcgattta gccgttgtcg atgccgatgg ttatgtcaat atcgtcgatc gcaagaaaga
      301 tatgattatc tccggcggcg agaatatcta ttccaccgaa gtcgagcatg ccctccatga
      361 tcatccgcag attatcgaat gtgccgtctt cggcgtaccc gatgagaaat ggggcgagtc
      421 aatcaaagcc tgcgtggtta ccaaggccgg tgcagtcttg aaagaagagc aggtaattga
      481 gttcgtcaag gagcgactgg ctcactacaa ggcaccacgg gaagtggagt tcatggatga
      541 actgcctaaa accggtagcg gcaagatcct taagaaggta ttgcgcgaca ggtgttggca
      601 gggacgagtc aagcgcgtca attaacagct ctgatagttg ctatagaccg cacagttacg
      661 tatactttgc tgtgagtaaa gtatacgtag ctatctgttt gccagcttga actattaccc
      721 tatatctaca aagagcctaa tgactaagca aaatatagcc actattggca gtaccaggaa
      781 accccttacc tgtttgaaag ctaaagtgat atatgcgcta attgtgcttt ctctatacac
      841 tacctgtggt gccctggctg ctcctacggc agagagtcag accagcatgc agcagttcaa
      901 agtagccaaa gctgcttaca tgcagggcga gtatgagctg gcaaagaagc atttgcttgc
      961 cgctgtggac agcgataagt ctaattggga agcccactat ttgctcggtc ttgtactggg
     1021 tcaggaaggc aatttcagct tgagtatcgc ccatgcccag aaatccctgg ccgttaatcc
     1081 ggacttcgcg ccggcatatt cggttctggg ccgttctctg gctgaagccc agaagttacc
     1141 ggaggccagg caggcgctgg agacagcctg tcggctggat cctaagtcgg caggcaatca
     1201 gtgtaatctg ggtgccgtgc tgggcatgag taaggatttt aagggagcct acgctgctta
     1261 taaaaaagca gccgaacttg atcccaagta tgtgaatgcc tatctaggta tgtcatcgtg
     1321 cctgggaaaa cagggcgata agaaagggca gatcgaagct tgccgcaagg cctgcgaaat
     1381 ggtgccaggt tcagctctcg ctcatgccaa gttggggctt ctcctttctg aaaacggtga
     1441 tatcggcggc tctatgtcgg aaggatatac ggccaatgtc ctcaggttga aggaatcatg
     1501 gaatgacttc ctgggaacct tccttacggc ctgggcctcc gtatttctgg ttttcgccgg
     1561 aatctttgcc gttatctttg ccggctccaa gttcaagcct caggaagggg aagcagtgct
     1621 gcgttccttc ttccttactt tttacaaaga caagcccggt cgctttgtgg tgaccagcga
     1681 ccgcctggta tttgtgccgg aagctttttc agcctggttt ggctctacaa atgtcagcat
     1741 tcagagaggc cagattgaga gtatcaatta tctcagcacc gttggtggtg gcactgtttc
     1801 aatcctgacc agagacggca gtgtgcatca gtttcggatg cctctcctgg tccttgaccc
     1861 gctccgttct ttgcttgtcg ggcaaggcat agtttctaaa gagccttccg cggaagctcc
     1921 tgctgccgcc actgcagaag agaagaaaga agagaagaaa gaagagccgg ccgctcctgc
     1981 aaagactgaa gagacg
//
LOCUS       DAOQNZ010000809         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38208_length_1994_cov_3.318721, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000809 DAOQNZ010000000
VERSION     DAOQNZ010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38208_length_1994_cov_3.318721"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PKW73_13610"
     CDS             complement(<1..263)
                     /locus_tag="PKW73_13610"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNA74376.1"
                     /translation="MARISTLDLDKAEPKAKEILDGVNKALGTVPNVFKTFAHSPAVL
                     EAYMGFSGAMAKAKLPATVREQIALTVAGKNECDYCASAHTVLG"
     gene            complement(430..1710)
                     /locus_tag="PKW73_13615"
     CDS             complement(430..1710)
                     /locus_tag="PKW73_13615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA74377.1"
                     /translation="MAKTVEPSIQLQKEAAVQETPPFNAEKPLASEEGLARAAGYLTL
                     IAGLNGGWLGPLIPSIATAQHLPLEQVGLIVSFLCGGCFVAQLLGPGLIAAQSSRKTL
                     MLGSILLGLGMLALGLIPGLFAILTAAAVIGLGTGFNSIAGHVSILTYFRERAASALS
                     KLNIFYGVGALAAPVIVQFSGASLGSGYSHLLACAAALTLFVLIGLLRLPPEKEKQGK
                     NLKQTETRSVAVVTLPLILTCLVIFLYVGAETATGTWLFTYIIDSQTKDTRLASLTVT
                     ALFSGLTIGRLISIKISSLFSAPRVTSTAMVLMVLGLFLLSSIKAAALPALLLTFAVG
                     LGCGPIFPQMIAQASKLSPLQSLVPRVTAMAIGAGTIGGIIMPGAAGQVLARVGVSQT
                     MLSIALLALSMLSLYLFTLVVASKRKGERKSEQV"
     gene            complement(1801..>1994)
                     /locus_tag="PKW73_13620"
     CDS             complement(1801..>1994)
                     /locus_tag="PKW73_13620"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNA74378.1"
                     /translation="LARAHARSGDAAVISGYLSDESTFDEAIGSFAMAYADQAEKDYE
                     EFAAAVRSGRLPVAEDLGL"
BASE COUNT          453 a          513 c          594 g          434 t
ORIGIN      
        1 ccgagcacgg tgtgggccga ggcgcagtag tcgcactcgt ttttgccggc gactgtgagg
       61 gctatctgct ctcttacggt ggcgggcaat ttggctttag ccatggcgcc gctgaagccc
      121 atgtaggctt ccaaaactgc cggtgaatgg gcgaaagttt tgaagacatt gggaacggtg
      181 ccgagggctt tgtttacgcc gtcgagaatt tcttttgctt tcggttctgc tttgtcgaga
      241 tcaagtgttg agatacgagc catggatcct ccaggatgtc agagaaaagt acttgagccg
      301 gtctacttgc gtcgaaatgg gcctatgcac caagcgtgca gccgccagat caatcgctcg
      361 tttactgacc ggttcaattt tgctttatcg atgcatctta ggtgcttgac tggaattagt
      421 caactacagt cagacctgtt cacttttccg ttcgcctttg cgtttgctcg caacaacgag
      481 agtaaataga tagagggaaa gcatagatag cgccagcagt gctatggaaa gcatggtctg
      541 gctgaccccc acccgggcca gtacttgccc ggctgctccc ggcatgatga taccaccgat
      601 agtgccggcg ccgatggcca tggccgtcac tcttggtacc aggctttgta gcggtgaaag
      661 tttggaggcc tgagcgatca tctgggggaa aatgggacca caaccgagac cgacggcaaa
      721 ggtcagcagc agggccggta aagctgccgc tttgatagag gacagcaaga acagtcccag
      781 aaccatgagg accatggctg tgctggtcac ccgtggggca gagaagagag aggatatctt
      841 tattgagatc agtcgcccta tggtcagccc gctgaagagg gcggtgacgg tcagggaagc
      901 cagtctggtg tctttggtct ggctgtctat gatataggtg aagagccatg tacctgtagc
      961 tgtctcggcg ccgacataga gaaaaatcac caggcaagtt agtatcagtg gtagtgtcac
     1021 aacagcgacc gacctggtct cagtctgttt cagattcttc ccttgctttt ccttctccgg
     1081 cggcagtctt aagagaccga tgagaacaaa aagggtgagg gcggcggcgc aggccagcaa
     1141 atgactatag cccgagccca ggctggcgcc ggagaactgc acaataaccg gagcagccag
     1201 ggcgccgacg ccgtaaaaaa tattgagttt actgagcgcg ctggccgctc gctcccgaaa
     1261 ataggtaaga atggagacat gaccggcaat gctgttgaag cctgtaccca gaccgataac
     1321 ggcagcggcg gtgaggatgg cgaaaagacc ggggataaga cccagagcca gcatacccag
     1381 ccccaggaga atgctgccca gcatcagggt cttcctgctg ctctgggctg cgattagacc
     1441 tggtcccagt agttgggcta caaaacagcc gccgcaaaga aagcttacaa tcaatccgac
     1501 ttgctccagg ggcaggtgtt gcgccgtggc tatggaagga atcaagggcc caagccagcc
     1561 gccgttgaga ccggcaatca aggttagata gccggcggct ctggccagcc cttcttccga
     1621 agctagaggt ttttcggcgt taaatggcgg tgtctcctgc acggctgctt ctttctgaag
     1681 ctgtatggat ggttcgactg tttttgccaa ggctgagacc tgaaggagag aaattaattc
     1741 tttcatccag ggggtggtag gcagcctcgg gcctgatcag tcttgaggaa actttaccgg
     1801 ctagagaccc agatcctctg ccacaggcaa tcttcccgag cgcaccgccg cggcgaattc
     1861 ttcatagtct ttttcggcct gatcggcgta agccatggca aaactgccga ttgcctcgtc
     1921 gaaggtgctc tcatccgaca ggtagccgct tatgacggcg gcatcaccgg agcgggcatg
     1981 ggccctggcc agta
//
LOCUS       DAOQNZ010000810         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38363_length_1990_cov_3.381912, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000810 DAOQNZ010000000
VERSION     DAOQNZ010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38363_length_1990_cov_3.381912"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="PKW73_13625"
     CDS             complement(<1..1330)
                     /locus_tag="PKW73_13625"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74379.1"
                     /translation="MRKAVSLACTILLSTTSSLSAALAFPQRHSTLEPLVLANTQGQA
                     KSEHKPDFKPQMPNQSNMAPKETTDKSIGAEALIKELQKLTDVQQTPQDAEKIKKLEK
                     EGQKLFDQKMFGQALAVWQEGYGLCLDTKNSEGQGRALTNMAKVYIEQGQWIKAKHLG
                     ENAIEVLTGGGNEVVLAQARIVLAQAYFGLDNPVWATQQLDAALRSLSSGQLNDPDEG
                     SRLMYLCANLAVKSGKVKEAIKFYQQGAQYSYDKGDIGKAVQVRATIVNSMIDLGWFV
                     AALEEANKLLSVAKAAPKDSNVYQITGYQCIGNAHFAAGEFANARRSYETVYALISKI
                     DARQIPDVSRANIDEGYGFVLCACGDYEQGRQMLLKAASVLKAKQDNQGLAQIYNALG
                     VIEVQDGQTGKGLGYFQQGLDYHAVLQPVLPRLHAITMQNMACAEYRSGSF"
BASE COUNT          484 a          532 c          511 g          463 t
ORIGIN      
        1 ggaaggagcc gctgcgatac tcggcgcaag ccatgttctg catggtgatg gcatgcaaac
       61 ggggcaggac cggctgcaat accgcatggt agtcaagtcc ctgctgaaaa tagccgagac
      121 ctttaccggt ctgtccgtcc tgaacttcaa tgacaccgag ggcattgtag atttgagcca
      181 gaccctggtt gtcctgtttg gctttcaaga ccgaagcggc tttcaagagc atctgccggc
      241 cctgctcata gtcgccgcag gcgcaaagca cgaagccata accctcatcg atgttagccc
      301 tggatacatc aggaatctgt ctggcatcga ttttggaaat gagagcatag acggtctcat
      361 aagaccggcg agcattggca aattcgccgg cggcaaaatg ggcattgccg atgcactgat
      421 aaccggtgat ttgataaaca ttggaatctt tgggagcggc tttggctacc gagagcagtt
      481 tgttggcttc ttccagggca gccacgaacc aacccaggtc gatcatggag ttgacgatgg
      541 tggctctcac ctgcacggct ttaccgatat cgcctttgtc atagctgtac tgagcgccct
      601 gctgatagaa cttaatggct tccttcacct tgccggactt gaccgccaga ttggcgcaaa
      661 ggtacatcaa gcgggagcct tcatcggggt cgttcaactg accgctggaa agggagcgca
      721 atgcggcatc aagctgctgt gtagcccaga ccgggttatc cagaccaaaa taggcttgcg
      781 ccaggacgat acgggcctga gccaggacca cttcgttgcc gccgcccgtc agaacttcaa
      841 tggcgttctc gcccaggtgc ttggccttaa tccactgccc ctgttcgata tagactttag
      901 ccatattagt cagggctcgt ccctgacctt ccgaattctt ggtgtccagg cagagtccgt
      961 agccctcctg ccagaccgcc agagcctgac cgaacatttt ctggtcgaag agtttctgcc
     1021 cttccttctc aagtttcttg attttttcag catcctgagg ggtctgctgc acgtcggtca
     1081 gtttctgtaa ttctttgatg agggcctcgg caccaatcga cttgtctgtt gtttctttag
     1141 gcgccatgtt tgactggttg ggcatctgcg gcttaaagtc gggtttatgc tcagacttgg
     1201 cctgcccctg ggtgttagca agcacaagcg gctccagggt ggaatgcctt tgagggaagg
     1261 ccagagccgc actcaggctg gaagttgtcg aaagaaggat cgtacaggct agtgaaactg
     1321 cttttctcat tgattcaaat tcaactcaat tgacgtttgg atggctgatc taccctttcc
     1381 taccagagct cctgcaaaaa tgacagagag ttgggaagca aggtccaagg caaataagaa
     1441 tagtcaatca gggtcgattt tgcctgtgaa aacaaccata aataaacaga gatgggcaaa
     1501 aaataaggtt gcgaagagat actaaaattc gcttcaccat ggggaacaaa gctgttcttg
     1561 gcgcgtcttg cccttttgtt gatcatctcc gccagcaaga acaaaaacct tgttaattgc
     1621 cgacgaacct agaaagagag ccctgaattc ggtgccactt gcaccagcaa acttgttaaa
     1681 cgcaggcccg gcgcgtctga tatatcggca accggcaagc ctccagagca gcacaaatcg
     1741 gctcaaaaca agggatcacg gagcaatcgg ctgcgcctcg cagctgccac agatggtaac
     1801 ctttagacag ctctagataa cagcagagcc aacttgacca agtaacttaa caaccacaga
     1861 gaccaaacta gatattggag tatcccatga gaaaccttcg ccaagttacc cttgccgcac
     1921 tcatcgccct tattggtacc accagccagg ccgctttcat tccggcccag gcagacaccc
     1981 tctcggcagg
//
LOCUS       DAOQNZ010000811         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38414_length_1989_cov_2.812823, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000811 DAOQNZ010000000
VERSION     DAOQNZ010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38414_length_1989_cov_2.812823"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..609)
                     /locus_tag="PKW73_13630"
     CDS             complement(352..609)
                     /locus_tag="PKW73_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74380.1"
                     /translation="MNNKLFVGNLSFKVTETELQEVFAQAGEVVSVSIPTDKYTGKKR
                     GFGFVEMASQEAAEEAIKALNGRALAGREMSVNLARPKAAK"
     gene            874..1353
                     /locus_tag="PKW73_13635"
     CDS             874..1353
                     /locus_tag="PKW73_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74381.1"
                     /translation="MVLLAPVTYAGEGVSDPSPYKIEPVKSKSPLDDQVSLSEHQSGK
                     GGTTIYIKRVRGIGATRVLPTGRTKVPLALRFKGFGSLENLSLQSRHVRIHTSLGRNP
                     WVVTEYCPGEDGHWVAASKTIRWRLRREKGDIVTDVPQALLAEAEGDLLVSWIDAYR"
     gene            1363..>1989
                     /locus_tag="PKW73_13640"
     CDS             1363..>1989
                     /locus_tag="PKW73_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74382.1"
                     /translation="MRLKVLPALVAAFAAFSPASAEPGSPVVAEFFAGPKAAAGSAKK
                     AVESADAFFSGLKKAFPNESLILLRENGEVSGLSRTSADFLTYRNASYRITNKLPLNG
                     QPLLILDGVMPVKSESGAVSTALAQLKKERRQPMKLEAMRSKERMKVKVDVVFREHFS
                     EGTRILLLVMESAPVPAPVLQIVDLGYLTPGHRGSLEQVIKLDKSWFDG"
BASE COUNT          417 a          482 c          568 g          522 t
ORIGIN      
        1 gttggccaga atcaccagga cgccggcttt gaaggcggcg ctggcggcaa ttttgcccat
       61 gagagaggga cgccggaggg cgctttcctg cacctcgggg ctggttgagg tttccctggt
      121 cttgctcgtt gtgagcggca tcttctacct tttgccttga caagccttga attctaacaa
      181 aattgaggcg gcagaggttg ctaaaaggtg ctgagctaag ccagattaca gatattagtt
      241 attttacctg cgaaaaaatc cccgcaaaaa gccgcaaggt tcttgcgaaa gagcctttac
      301 tgatgctttt gcggggatta agtcaggacc ttaaatcgct cggatctgag gttacttagc
      361 agctttcggg cgagccaagt tgacggacat ttcacgtccg gccagggcgc gtccattcag
      421 agctttgatg gcttcttcgg cggcttcctg ggaagccatt tcaacgaagc caaaaccacg
      481 cttcttaccg gtgtatttgt cggtggggat ggacaccgat acgacttctc cggcctgcgc
      541 gaacacttct tgcagctcgg tttcagtcac tttgaaagag aggtttccaa cgaatagctt
      601 attgttcatc tgatctcgta gcaatgtccg gcatcctgac actaactgat accggtaacc
      661 caaataatgc tgagggttca ggtttagtat ctcatagtta tttattagtg cctagtagct
      721 cctctaaaaa gcgtttccgt gccccgtctg ttaccatggt agatgcccgt tagctccgct
      781 gggtgcctgc ttatggcgat tctcaaaatg aagattccat ttttttcagc tttccaaatt
      841 cgtcggcttt ttcctttttc tctgccggcg tttatggtct tgcttgcgcc cgtaacctat
      901 gccggggagg gggtttcgga cccttctcct tacaagatcg agccggtaaa aagtaaaagc
      961 cccctggatg atcaggtttc gctctctgaa caccagtcag gcaagggggg caccacaatc
     1021 tatataaagc gcgttcgagg tataggtgct acccgggtct tgccgacggg gcggacgaaa
     1081 gtgccgctgg cgctccgttt taaagggttc ggcagtctgg agaatctctc tttgcagagt
     1141 cgtcatgttc gcattcatac aagcctcggt cgcaatccct gggtggtgac cgagtattgt
     1201 ccgggtgagg acgggcattg ggtggcggcg tccaaaacaa tcaggtggcg gctcaggcgg
     1261 gaaaagggcg atatagtgac ggatgtaccg caggctttgc tggccgaggc ggaaggtgat
     1321 ttgcttgtct cttggattga tgcttataga taggtggttg ttttgagatt gaaagttctt
     1381 ccggctctgg ttgcggcatt cgctgccttt tcgccggctt cggcggagcc gggttcgccg
     1441 gtggtggcgg aattttttgc cggtcccaaa gccgctgcag gaagtgctaa aaaggcggtt
     1501 gagtccgcag atgccttttt ttcaggactc aagaaagctt ttcccaatga gagcctgatt
     1561 ttgctgagag agaatggcga agtgagcgga ctttcgcgta cttcggcaga ctttctcacc
     1621 tatcgaaatg ccagctaccg tatcaccaat aaattgccgc tcaatggcca gccgctgctc
     1681 atactggacg gtgttatgcc ggtgaaaagc gagtctggtg ccgtgtctac cgcccttgct
     1741 caattgaaga aagagcgccg gcagcccatg aagctggaag ccatgcgcag caaggagcgc
     1801 atgaaagtaa aggtagatgt tgtcttccgt gagcattttt ccgagggcac aagaattctg
     1861 cttctggtga tggagagtgc gccggtgcct gcacccgtgc ttcagatcgt tgacctgggc
     1921 tatcttacgc cggggcaccg cggctctttg gagcaggtca ttaaactgga taaatcctgg
     1981 tttgacggg
//
LOCUS       DAOQNZ010000812         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38462_length_1987_cov_5.132505, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000812 DAOQNZ010000000
VERSION     DAOQNZ010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38462_length_1987_cov_5.132505"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1155
                     /locus_tag="PKW73_13645"
     CDS             430..1155
                     /locus_tag="PKW73_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA74383.1"
                     /translation="MDNSSEQEKVLVVDDEASIRRILETRLKLAGYNVATAADGQEAL
                     EQFNTFQPDLVILDVMLPKMDGYEVCREIRKTSDTPIIMLTAVADVSNRIQGLEIGAD
                     DYVVKPFSPSELEARIKAVLRRTVERHQEVGQSKSTNVITIGNLKIDLNKRQVTRKNE
                     RIRLTGMEFSLLELLVTNSGKPYSRGEILQQVWAYPPDHRIDTRVVDVHISRLRSKLE
                     EDSSNPDLILTARGIGYMFQKIN"
     gene            complement(1243..>1987)
                     /locus_tag="PKW73_13650"
     CDS             complement(1243..>1987)
                     /locus_tag="PKW73_13650"
                     /inference="COORDINATES: protein motif:HMM:NF017071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNA74384.1"
                     /translation="VAVNDAQTKHMFDNRYGTGQSTLDGIIRATNRLVAGRTVVILGY
                     GWCGKGAAMRARGLGANVIVTEVDPIKGIEAVMDGFRVMPIAEAAALGDIFITVTGNR
                     HVIDAPHFEKMRDGAIVCNSGHFDLELNLTALEKMAKHKKEVRPLMEEFELKNGNRVY
                     VLAQGRLVNLACAEGHPASVMDMSFANQALALEYLVKNKGKLEHKVHTLPAQVDQEIA
                     ALKLKSMGVKIDTLSSEMTEYMNSWKTGT"
BASE COUNT          497 a          519 c          497 g          474 t
ORIGIN      
        1 cgccttatcg catcagatat ccctcgcaaa agcttggcat atacacacag caccgcctgt
       61 ggggacatga ttttctgcta atttcaaaga tagccggaac aaatatcggc gcttgcctta
      121 tcggacacag gtcttgattt cagcgccccg cctgacaacc aggctcgcca tcttccttat
      181 atatgagtga attggctcgc ccccactctg gagcgaggtc ggcacaatgt gggccggtga
      241 gcgccgtgca agaggggcgc cgccattgtc aagcattgca aaaggagaca cggcaatccc
      301 tgcccgtcct taataagtgg tatattcaga caagcattac taagggagca tcggctccat
      361 atttgtcccc gagccctggt gctcaaaaca gccgtaacta gcagttcaat agcaggaagg
      421 actttagctg tggataactc ttccgaacag gaaaaggtat tggtagtaga cgacgaagca
      481 tctattcgtc gcattctgga aaccagattg aagctggcgg gctacaacgt ggccacggct
      541 gcagatgggc aagaagccct agagcagttc aacacttttc aaccagacct ggtcatcctg
      601 gacgtcatgt tgccgaagat ggacggctac gaagtttgcc gcgagattcg caagacttcc
      661 gataccccca tcatcatgct caccgccgtc gccgacgtat ccaatcgcat tcaaggtctg
      721 gaaataggtg ccgacgacta tgtggtcaaa ccgttcagcc ccagcgaact ggaagcccgt
      781 atcaaagccg tactgcgccg cactgtcgaa agacaccagg aagtcggtca gagtaagagc
      841 accaatgtca tcaccattgg caacctcaag attgatttga acaagcgtca ggttacgcgc
      901 aagaatgaaa gaattcgttt gaccggtatg gaatttagtt tgctggaact tctggtaaca
      961 aattccggca agccttattc acgtggtgaa attctgcagc aggtatgggc ttatccgccg
     1021 gaccatcgca ttgataccag agtagtagac gtacatatca gccgcctccg ctccaagctg
     1081 gaagaagatt cctccaatcc ggatttaatc ttgactgccc gcggcatcgg ttacatgttc
     1141 cagaaaataa attaaccgcc tgcctgcagc gctgaagtta aagaaagaag aggacctttg
     1201 ccggtcctct tctttcttta acgaataact gcctgccgga acttatgttc cggtcttcca
     1261 ggaattcatg tattcggtca tctcagaact gagagtatcg attttgacac ccatagattt
     1321 gagtttgaga gcagcaattt cctggtccac ttgtgccggc aaggtatgta ctttgtgctc
     1381 gagtttacct ttgtttttga ccaggtattc aagggctaga gcctggttgg cgaaactcat
     1441 gtccatgacg gaagcgggat ggccttcggc gcaggccagg ttgaccagac gtccctgagc
     1501 caggacgtaa acgcgattgc cgtttttcaa ttcgaactct tccataagag gtcttacttc
     1561 ttttttgtgc ttggccattt tctcaagggc ggtcaagttg agttctaagt cgaagtgacc
     1621 ggaattgcaa acaatggcac catctctcat cttctcgaaa tgaggagcgt cgatgacgtg
     1681 gcggttgccg gtgacggtga tgaagatatc accaagggca gccgcttcag caatcggcat
     1741 cactctgaaa ccgtccatta ccgcttcaat acctttgatg ggatctactt cggtaacgat
     1801 gacattggcg ccgaggcctc tggcccgcat ggcggcacct ttaccgcacc agccatagcc
     1861 aagaatcaca acggtgcgtc cggcaaccaa cctgttggta gcgcggataa taccatcaag
     1921 agtggactgc ccggtaccgt agcggttatc gaacatatgc ttggtctgcg catcgttgac
     1981 cgctaca
//
LOCUS       DAOQNZ010000813         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38528_length_1985_cov_3.645078, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000813 DAOQNZ010000000
VERSION     DAOQNZ010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38528_length_1985_cov_3.645078"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKW73_13655"
     CDS             complement(<1..683)
                     /locus_tag="PKW73_13655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74385.1"
                     /translation="MTSGKDKFNLQKDEQGFIGKDSFVGKVIDHYEVEEKIAEGGLSA
                     VYRATDTNNRQTVVLKIIHKHLISSIKNYKKLEQKIRALINLNDPHIATYKDVVFADG
                     RVVLVMKPFIFESLEDLLSKTGHIGPERAVGIFIQVCQAMEAANKADIVHRDLKPSNI
                     IILDNQKFSDDIMVLDFGIAKIIADETEGGKSEQFMTRTRETFGSPLYLSPEQCAGKK
                     VDLRSDIYSL"
     gene            complement(700..>1985)
                     /locus_tag="PKW73_13660"
     CDS             complement(700..>1985)
                     /locus_tag="PKW73_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74386.1"
                     /translation="EKPDLSLLERMAAESEAYAMLKTIVERCLEKDPNNRFADMEELE
                     ESLLAIPLVKESDNKNRKPDRNEKDVRLLIFSLVTAAVATVSVMSLAVYSLWPVARVQ
                     EHLLQLKLPLPPLSYRPAVPPGLMDRKTKILMNDSSLSLVSGDVKAPNGQTLKSQVQT
                     LLAAAALFLEEKNYLEAGSRYSKAYERLKEFDKCTYDQGGSGNRRDRLVSATGAVWTY
                     YQLDRLKKQRALASGLAVSSRPSRELVESEHTLEDLLDEAEIVEKGDPLCLNRAYTVF
                     AMVLEDQQDLVKAMEVVNKDLKLDREVVDGDPFTPYGWALKKANLAELCRITEKFDRE
                     KDPYENALADLEKAGYGKTPQEEKVMAQLRFHHGLYLLELEHAGAYKAAFEEFSRALP
                     TLSGVERSACAGYAYESIKHYDWFGSLLFRFKNAI"
BASE COUNT          467 a          533 c          474 g          511 t
ORIGIN      
        1 agcgaatata tatccgagcg caaatcaact tttttaccgg cgcactgttc cgggctaaga
       61 tagagaggac tgccgaaggt ttcccgcgtg cgagtcataa attgttcaga cttgccgcct
      121 tcggtttcgt cggcaattat tttggcaatg ccgaaatcga gaaccataat gtcgtcggaa
      181 aatttctggt tatcgagaat aataatattg ctcggcttca aatcccggtg cacgatatcg
      241 gctttattag ccgcttccat ggcctggcag acctgaatga aaatgcccac ggccctttct
      301 gggcctatgt gtccggtttt tgaaagcaga tcttcgagac tttcgaagat gaagggcttc
      361 atcaccagta ccacccgccc atcggcaaag accacgtctt tgtaggtggc aatatggggg
      421 tcattgaggt taatgagagc ccggatcttc tgctccagct tcttataatt tttgatggaa
      481 gaaatcagat gcttgtggat tatcttcagt acgaccgtct gccgattgtt tgtatcggtg
      541 gctctgtaaa cggcactgag tccgccttcc gctatcttct cctctacctc ataatggtct
      601 atgactttac cgacaaagct atctttgccg ataaaaccct gctcgtcttt ttgcaggttg
      661 aacttgtctt ttcctgaagt catgctttaa ctcattcacc taaattgcat ttttgaatct
      721 gaacaaaagc gagccaaacc aatcataatg ctttatggac tcatatgcat aaccggcgca
      781 ggctgaacgc tccactccgc tcaaagtagg cagggcccgg gaaaattctt caaaggccgc
      841 cttataggca ccggcatgtt ccaactccag caaataaagt ccgtggtgaa agcgcagttg
      901 cgccatcact ttctcttcct ggggcgtctt tccgtaaccg gctttctcaa ggtccgctaa
      961 ggcattctca taggggtcct tttccctatc aaatttttcg gtaattcgac agagttccgc
     1021 cagattagct ttcttcagag cccagccgta gggcgtaaag ggatcgccgt ccacaacctc
     1081 cctgtccagc ttcagatctt tattgaccac ttccatagcc ttaaccaggt cctgctggtc
     1141 ctccagaacc atggcaaaga cagtgtaggc gcggttcaag cagaggggat cacccttttc
     1201 gacaatttcc gcttcatcca gcagatcctc gagggtgtgc tcgctttcca ccagttcccg
     1261 actgggccgg gaagaaaccg ccagaccgga agcaagggca cgctgcttct tcaaacgatc
     1321 caactgataa taggtccaga ccgcaccggt ggcactgacc agcctgtcgc gccgattgcc
     1381 gctgccacct tgatcatagg tacatttatc gaattccttc agccgctcat aggctttgct
     1441 gtaacgagaa ccggcctcca aataattctt ctcctctaga aagagggcgg cggcagcaag
     1501 aagagtctgc acctgacttt tcaaagtctg tccgttggga gccttgacgt cgccggaaac
     1561 cagagagagg gaactgtcat tcatgagaat cttcgtcttc cggtccatga gacccggtgg
     1621 cactgccggt ctataactga gaggcggcaa aggcaacttc aactgcagca aatgctcctg
     1681 taccctggct acgggccaga gtgaatagac ggcaagggac atgacagaga cagtggcaac
     1741 cgccgccgtc acaagtgaaa atatgaggag cctcacgtcc ttttcgtttc tgtcaggctt
     1801 gcggttctta ttgtcagact ccttaacgag aggtatggca agaagagact cttccagttc
     1861 ttccatgtcg gcaaagcgat tgttggggtc cttctccagg caacgctcca cgatggtctt
     1921 gagcatggcg tatgcttccg actcagccgc catgcgctca agaaggctca gatcaggctt
     1981 ttctt
//
LOCUS       DAOQNZ010000814         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38559_length_1984_cov_4.386210, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000814 DAOQNZ010000000
VERSION     DAOQNZ010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38559_length_1984_cov_4.386210"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="PKW73_13665"
     CDS             complement(<1..1898)
                     /locus_tag="PKW73_13665"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNA74387.1"
                     /translation="MPSFEQEKEKAAAAATERPADKELQEQSEKAPLKPLDFSPVPDE
                     RALQLTALKPAGNTGGDKASPFVPALDLVSERTLTYPGQKAESAAKNLYENGELKQAR
                     EQALKEGLPLLVDVGASWCAPCRALGRELHANEKELAGKAKVVKLDIDKLNSDPSTLS
                     AADQALVKEQKQILKELGIKPEEITGVPYVKLMALNKDGKPLGESKDMSGFPQGQAEG
                     RAWFNNFLAESSKLKKTMDQNNAPQEKPETKSKEEKPTEVLIKREEGEYKVDQQGRVT
                     STKSADGSLKREITYNGESLEVATARINDVEFRRTEGNNFHLSRLQGKEQAEDLGNWK
                     GNLTMTRSGLFGIQPEGESQAQYKDAGANLLSEGQLKLREKEGLTANESSAAGDKIQL
                     VQYQDSQKKKAESSEGSAASGSDNGTVPSKTIKLEEGTYERNDAGQVIKSTSADGKTV
                     REFKYEDKDKPDRVTSFTVNGEREFRFVGNNKYSDGSVVKKDGHEISSYSIYEKGQLT
                     GNWSGAIEMSKNGVLTTGDGSKELSHLDASSRALSEEERQKREASGIWPNKLTATLSD
                     GSTLTGRYQGSTLNELIETRMEGGLLVEASKWERKGDTFTNPAFPGEVRKNMTLQADG
                     TLSFTDKEG"
BASE COUNT          295 a          572 c          522 g          595 t
ORIGIN      
        1 ccctctttgt cggtgaaaga gagcgtgccg tcagcttgca aggtcatgtt cttgcgaact
       61 tcccccggga aggccggatt tgtaaaggtg tcgcctttcc tttcccattt gctggcctct
      121 accagcaggc cgccctccat gcgggtttct atcagttcgt tgagggtgct gccctgatag
      181 cgtccggtca gggtgctgcc gtcactgagg gtggcggtca gtttgtttgg ccagatgccg
      241 cttgcctctc gcttttgccg ctcttcctcg ctgagggctc ggctgctcgc gtctaaatgt
      301 gagagttcct tgctgccatc gccggtggtg aggacgccgt tcttactcat ttcgatggcg
      361 ccgctccaat ttcccgtgag ctgacccttc tcgtagatgg agtaggaaga tatttcatgg
      421 ccgtccttct tcactaccga accgtcggaa tatttattgt tgccgacaaa gcgaaactcg
      481 cgttcgccgt taacggtgaa ggaggtgact ctgtcgggtt tatccttgtc ctcgtacttg
      541 aattccctga ctgttttgcc gtcggcggag gtgcttttga taacctgacc ggcgtcattc
      601 ctctcgtaag taccttcctc cagcttgatc gtcttagacg gcaccgtgcc gttgtcgctg
      661 ccgcttgccg cagacccctc gctgctttcg gctttcttct tctgactgtc ctggtattgc
      721 actaactgta ttttgtcgcc ggccgcgcta ctctcattgg ccgtcagacc ttccttctct
      781 ctcagtttca attgcccctc gctgagcaga ttggcgccgg cgtccttata ctgagcctga
      841 ctttcgcctt caggctgaat accgaaaaga ccggagcggg tcattgtcag attgcccttc
      901 cagttgccca gatcttctgc ctgctccttt ccttgcaggc ggctcagatg aaagttgttg
      961 ccctcagtgc ggcgaaattc cacatcattg atgcgggccg tggccacttc cagtgattct
     1021 ccgttatagg ttatctcccg cttcagtgag ccgtcggctg atttcgtcga tgtgactctg
     1081 ccctgctggt cgaccttgta ttcgccctcc tctcttttga tcagtacttc ggtcggcttc
     1141 tcctccttgc ttttggtttc cggtttctct tgcggagcat tgttttggtc catggtcttc
     1201 ttcaacttgc tgctttcagc aagaaagttg ttgaaccagg cccggccctc cgcctgtcct
     1261 tgagggaaac ccgacatgtc tttgctctct ccgaggggct tgccgtcttt gttcagggcc
     1321 atgagtttga cgtagggcac accggttatt tcctccggtt tgatgcccag ttctttcagt
     1381 atctgcttct gctccttcac gagcgcctgg tcggccgctg aaagcgtcga cggatcgcta
     1441 ttcaatttat cgatgtccag ctttaccact ttcgccttgc cggccagttc tttttcgttg
     1501 gcgtgtaatt cgcgacccag ggcgcggcag ggcgcgcacc aggaggcgcc tacatccaca
     1561 agcaggggca gaccctcttt cagggcctgc tctctggctt gcttgagttc gccgttttca
     1621 taaaggtttt tggcggcaga ctctgctttt tgaccggggt aggtgagtgt tctctccgat
     1681 accaggtcca gcgccggaac aaagggactg gccttatcgc cgcctgtatt tccagcaggt
     1741 ttcagtgctg ttagttgcag ggcccgttca tccggcacgg ggctgaagtc cagaggcttc
     1801 aacggcgctt tttccgactg ctcttgcagt tctttatctg cgggtctttc cgttgcggcg
     1861 gctgcagctt tctctttttc ttgctcgaaa ctgggcatgg ctatcctcta ctggggcgct
     1921 gggagaagat tttcaaagac ttttgcaaac tgattttagt ctggctttca ccaacactag
     1981 gggg
//
LOCUS       DAOQNZ010000815         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38563_length_1984_cov_4.116641, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000815 DAOQNZ010000000
VERSION     DAOQNZ010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38563_length_1984_cov_4.116641"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PKW73_13670"
     CDS             <1..511
                     /locus_tag="PKW73_13670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74388.1"
                     /translation="DIFSQACEALIAVHSQGMIHRDVKPGNLLLGKDGMIKLVDFGLC
                     KNVGGLENLIRLTCTGEVVGSPSYMSPEQCIGKELDQRSDIYSLGVSMYEALTGEIPF
                     DGDSFYEILNQHIRGTASREPFLRKNIPIELEEIVFRCIDRDPDARFQSAEELLKAIQ
                     KAAKLVGTK"
     gene            563..>1984
                     /locus_tag="PKW73_13675"
     CDS             563..>1984
                     /locus_tag="PKW73_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNA74389.1"
                     /translation="MLDFNARLKKLLNGAIWNTLESCQPELNQELGAALDELIILAES
                     EHEALNKLEQQFAIFIKLVEMCPSFLWIADSDGMVEYASERWMEFSGATEEQTLGTGW
                     MDFVDERDRESAVAEWRSSIQNTSTFELTLRLKSGLDGSSRWFLARGVPLADENGDTI
                     KWFGSTTDIHTQKTLKEELSTKVEDLSRLIEEYNQSQEKLVESENLFQVVCETSNHLI
                     ITTDEDGAVEYCNQRWKDYVGLSLRENAGKAICWFKIIHSDDRLKFDQHWQKAREQDT
                     PLEVEVRLKEKDGKYLWHRLCGMPIKCHNGKRWCITCTEVEAYYSLLESLSKAKEAAE
                     EASKLKSSFVANISHELRTPLNGVLGMTQLLRTLTLPEPADDYLNVIEEAGQSLLTVI
                     NDVLDFSKMEAGKMELVEEDLSVATIVDGATNILATQAEFKELFFLSYVDKRIPEILR
                     GDGQRVKQVLLNLLSNAIKFTEIG"
BASE COUNT          563 a          484 c          516 g          421 t
ORIGIN      
        1 ggatatattc agccaggcct gtgaagcact catagccgtg cacagccagg gaatgattca
       61 cagagacgtt aaaccaggca atttattgct tggcaaagac ggcatgatca aactggtaga
      121 ctttggactc tgcaaaaacg tcggcggtct ggagaatctc atccgcctta cctgtaccgg
      181 cgaagtcgta ggctcgccgt cttacatgag cccggagcag tgcatcggca aagaattaga
      241 ccagcgaagc gatatctact ccctgggagt atcgatgtat gaagccctca ccggcgagat
      301 tccctttgat ggagattcat tctatgaaat tctcaaccag cacattcgag gtacggcatc
      361 aagggagccg tttctcagaa aaaacatacc gattgaactg gaagaaatag tctttcgctg
      421 catagataga gacccagatg ctagatttca gagcgcggag gaacttctga aggcaataca
      481 aaaggcagct aaattagttg gtacgaagta aaaacaggta ctcacaggaa caggcaacca
      541 agtttgccgg gaatctaagg ccatgcttga tttcaatgcc agattgaaaa aattgttaaa
      601 cggagccatc tggaacactc tggagagctg ccaacccgag ctcaatcaag agctgggtgc
      661 ggcccttgac gagctgatta ttctggcaga aagcgaacat gaagctttaa acaaacttga
      721 gcaacaattt gccatcttca tcaaattggt tgagatgtgt ccgtccttcc tctggatagc
      781 cgacagcgac ggcatggttg aatatgccag cgagcgctgg atggagtttt cgggagccac
      841 cgaggagcaa accctgggca cgggctggat ggatttcgta gatgagagag atcgcgaaag
      901 tgccgttgct gagtggagat cgtctatcca aaacaccagt accttcgaac tgaccttgcg
      961 cctcaagtca ggtcttgacg gcagcagcag gtggtttctg gcccggggcg ttcccctggc
     1021 cgacgagaac ggcgacacca taaaatggtt cggcagcacc accgacatac atacccagaa
     1081 gaccctgaaa gaagaattgt ccaccaaggt ggaagacctt tccaggctga ttgaagaata
     1141 caatcagtct caggaaaaac tggtggaaag tgagaatctc ttccaggtgg tatgcgagac
     1201 ttccaatcac ctgataatta ccaccgatga agacggtgcc gtcgaatact gcaaccagcg
     1261 ctggaaagac tatgttggtt tgagcctgcg cgagaatgcg ggcaaggcta tctgttggtt
     1321 caagataatt cacagcgacg atcgtctcaa gttcgatcag cactggcaga aagccagaga
     1381 acaggacaca ccactggagg tggaagttcg cctcaaggag aaagacggca agtacctctg
     1441 gcaccgactg tgcggcatgc ccatcaagtg tcataacggc aagcgctggt gcattacttg
     1501 caccgaggtt gaagcttatt acagtttact tgaatcctta tccaaagcca aagaggcagc
     1561 ggaagaggct tctaaactca agtcatcctt cgtggccaat atctctcatg aacttcgcac
     1621 ccccttaaac ggcgtgctcg gcatgacaca gctactgcgc actctcaccc tgcccgagcc
     1681 ggccgacgac tacttgaacg tgatcgagga agccgggcag agcctgctta cggtcataaa
     1741 tgatgtgctt gatttttcca agatggaagc cggaaaaatg gagctggtag aagaagatct
     1801 cagtgtggcc accatagtag atggtgccac aaacatactg gcgacccagg ccgaattcaa
     1861 agagctcttc ttcctttcct atgtagacaa gcgaattccg gagattctca gaggcgacgg
     1921 acaaagggtc aagcaagtgc tgctcaatct tctcagcaac gccatcaaat ttaccgagat
     1981 aggg
//
LOCUS       DAOQNZ010000816         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38666_length_1982_cov_2.516866, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000816 DAOQNZ010000000
VERSION     DAOQNZ010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38666_length_1982_cov_2.516866"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..400
                     /locus_tag="PKW73_13680"
     CDS             29..400
                     /locus_tag="PKW73_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74390.1"
                     /translation="MWLLYTALMLALVVFVCLEARRNKAEQLEDERELRHTIAVNLGI
                     LKRELMELQEIAKVSNLAELPRAQANSLLAASEETANRVQSQYEQAGLEDLRLLITAL
                     FQAMNQATEARRTLGAIIPLI"
     gene            514..1794
                     /locus_tag="PKW73_13685"
     CDS             514..1794
                     /locus_tag="PKW73_13685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNA74391.1"
                     /translation="MLNLNQDQSQNSAQNKTLTDQVKALAEKHFERVRQLRRTIHQNP
                     ELSFSESSTSKLVAKELQELGYTVKTGIGGTGLTAELAPVDAKKNAKEKAATTAVIGI
                     RADMDALPIQEENPVAYCSNHKGVMHACGHDAHTACGLGAAMILADLHQSGLAGNLPT
                     KVRFLFQPAEECTNDSGKGGATLMMEDGALEGLSALVGLHVFPSIPTGMIGLKEGPLL
                     AACDTFDIKITGKGCHGAYPQDGIDAIVLAAQAVQAIQTITSRRKSALNPCVITLGGL
                     RSTTYAPNIVAEAVELTGTARYFDPQMSTLTRTELERALGVVRALGGDFELKYKHENP
                     PLINDPTMTEAVRQAASKLAGREQILEPGLQLGAEDFSFYCQHTKACFLVLGAMIEGD
                     IRTLHTPSFDIDEEALKLGSAILALSTLEFLQNN"
BASE COUNT          501 a          592 c          518 g          371 t
ORIGIN      
        1 ccgccctaca aactaacaga ggctcactat gtggctcctc tacaccgcac tgatgctggc
       61 ccttgtcgtt tttgtttgcc tcgaagcacg caggaacaaa gcggaacagc tggaggatga
      121 acgagaactg cgacacacca tcgcggtcaa tctcggcatt ctcaagcgcg agttgatgga
      181 attgcaggaa atcgcaaagg taagcaattt agccgagctc cccagagctc aggcaaacag
      241 cttgcttgcc gcttccgaag aaacggccaa ccgggttcaa agccaatacg aacaggccgg
      301 tctggaagac ttgcggctgc tcatcacagc tctctttcag gccatgaacc aggctacgga
      361 agcccgccgc actctcggcg ccatcatccc gcttatctaa ccccccgttg agggcggctc
      421 cgtttgacct aaaactcaaa cgagctgccc tctttttttg ctttaagttg tcaagcattt
      481 ataaaatagg cagcacccgc accggaaaaa cccatgctca atctcaacca ggaccagtct
      541 caaaattcag cccagaataa aactctgacg gaccaggtga aagcactggc cgaaaagcat
      601 tttgaaagag tgcggcaatt gagacggacg attcatcaaa atcctgagct gagcttcagc
      661 gaaagcagca ccagtaaact ggtggcaaag gaattgcaag aactgggcta cacagtcaaa
      721 accggcatcg gcggcactgg tctaacggct gagttggcgc ctgtggacgc aaagaaaaac
      781 gcgaaagaga aagccgcaac aacggccgtg attggtattc gcgccgacat ggacgccctg
      841 cccatccagg aagaaaaccc agtggcctat tgctccaacc acaagggcgt catgcatgcc
      901 tgtggtcatg atgctcatac ggcctgcggg ctcggcgccg ccatgatcct ggccgatctg
      961 caccagtcgg gactggccgg caatctgccc actaaggtgc gctttctctt tcagccggcc
     1021 gaggaatgca ccaacgacag cggcaagggc ggcgccacgc tgatgatgga agacggtgcc
     1081 ctggagggac tttcggccct ggtcggtctg catgtttttc caagtattcc aaccggcatg
     1141 attggcttga aagaagggcc gctgcttgcg gcttgcgata ccttcgatat aaagataacg
     1201 ggaaagggct gccacggggc ttacccgcaa gacggcatcg acgccatcgt gctggccgct
     1261 caggccgtac aggccattca aaccattaca tctcgccgta aatcagccct gaatccctgt
     1321 gtaatcaccc tgggagggct gcgttccaca acctatgctc ccaacatcgt ggccgaagca
     1381 gtggagctga caggcacggc ccgctacttt gacccccaga tgtctaccct gacccgcacc
     1441 gagttggagc gggccctggg agtggtaagg gctctaggtg gagatttcga actcaagtac
     1501 aagcatgaaa atccgcccct gatcaatgac ccaaccatga ccgaagcagt ccgacaggcg
     1561 gcaagcaaac ttgccggccg ggaacagatt ctggaacccg gactgcaact gggcgccgaa
     1621 gatttcagct tttactgcca acacacaaag gcctgcttcc tggtgctggg agccatgatc
     1681 gagggtgata tccgcaccct gcatacaccc agcttcgaca tcgacgaaga ggctctcaag
     1741 cttggctcgg ccatactggc gctgtcgacc ctggaattcc tgcagaacaa ctgaactact
     1801 tccaaaaggt catgatggaa ggccggcagc cacctcctga cagggcgccc accgcctgac
     1861 aataacgcta tttccaccaa gttgccaaca tgatgcagct gcgccacccg caaactacgg
     1921 gggtttttgc ctggcatccg gcgaaaaccg ccagagcaaa cagccgacta cttcttgcaa
     1981 ac
//
LOCUS       DAOQNZ010000817         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38767_length_1979_cov_3.355509, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000817 DAOQNZ010000000
VERSION     DAOQNZ010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38767_length_1979_cov_3.355509"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..>1979
                     /locus_tag="PKW73_13690"
     CDS             88..>1979
                     /locus_tag="PKW73_13690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74392.1"
                     /translation="MNGEFSPAKDETLADGSPPESAPLDLADSTCQLVSERYSLKELI
                     GKGGMGSVFRARDLLLDRDVALKLLPAHLNENAAALARFQREARAAAGIRHPAVVTVH
                     DFGLTESGQPFLVMDLIEGKTLKQLIEEQGQLSFAEIEQVFTRVIEGLKQAHGAGVVH
                     RDLKPGNIMLTDAGEQEALLLDFGIAKVGSGEGENSGVLNLTQTGEILGSPLYMSPEQ
                     CLGEDVDFRSDIYSLSCCLYEALTGEPPFKGKNAFETMTMHVNRSPASLSFSRPDLNF
                     GSQLQAVIERGLAKSREARYQSLEQFQSDLVSALRCSFDLALAGDGASPASSKAVLVD
                     WRSSNGLILLAVLLSVACIGLVFPLFLQLFLFKPLYGALGLIVVGVGLVFLSQLVFGP
                     AGLLVRSVQNRYVGESEASHRLLLKQLAPVSDQKGALELGSYLDFNLLLPRRQTIVVP
                     PSLRAGLSFALVLGKSGKGKTRLLRRLIRQDRVNGSIFVLDTQGTLFDGLQSEFSHAD
                     TVCFFDFSATAPELTASAHSPSASREQLSQLAAQCMVEALGGVSLMQKVNMPGLSKDK
                     EMALTEVLEGFFLEKIKVDELPSRLQASGVGSECSALILSLLTLPCFACLPALLPGCV
                     RAFETGG"
BASE COUNT          384 a          498 c          589 g          508 t
ORIGIN      
        1 tcaagatcca ggactttggg catcatacaa atgaacacag cgggtagaag gtataaattt
       61 actgcctgcc aggtgagagg agcgcctatg aatggtgagt tcagcccggc caaagacgaa
      121 acgctggcgg atggaagtcc gccggagtcc gctccgcttg acctggcgga tagtacttgc
      181 cagcttgttt ccgagcgtta ttccctgaaa gagttgattg gcaagggggg catgggcagt
      241 gtttttcggg cccgcgacct gctgcttgat cgcgacgtgg ctttgaagct tttaccggct
      301 catttgaacg agaatgcggc agccctggct agatttcagc gagaggcaag agcggcggcc
      361 ggcatcagac atccggccgt tgtgacagtg catgattttg gcttgaccga gagcgggcaa
      421 cctttcctgg tgatggattt gatcgaggga aagaccctca agcagctcat cgaagagcag
      481 gggcagctca gctttgctga aatagagcag gtttttacca gggtgattga ggggttgaag
      541 caggcgcacg gtgcgggagt tgtgcaccga gacttgaaac cgggcaatat tatgctgaca
      601 gatgcaggcg agcaagaggc tttgcttctc gacttcggca tcgccaaagt gggttcaggc
      661 gagggggaaa atagtggtgt tttgaatctc acccagaccg gtgaaattct tggtagtcct
      721 ctatatatga gtcctgaaca gtgccttggc gaggacgttg attttagatc cgatatctac
      781 tcgctcagct gttgtttgta tgaagcgctg accggtgagc ctcccttcaa ggggaagaat
      841 gcctttgaga ccatgaccat gcatgtaaac cgcagtccgg catctctttc ctttagccgc
      901 cccgacttga atttcggctc gcaattgcaa gcggtcatag aaagaggact tgctaaaagc
      961 cgagaggcac gctaccagag tcttgagcag tttcaaagcg acctcgttag cgccctccgc
     1021 tgttcctttg acttggcgct tgccggtgac ggtgcctcgc ctgcctcatc gaaggcggtg
     1081 ctggtggatt ggcggtccag caatgggctg atactactgg cagtgctcct cagcgtggct
     1141 tgcattggtc tggtcttccc ccttttcttg cagttgtttc tgttcaagcc cctctatggg
     1201 gccctggggc tgatagtggt gggagttgga ttggttttcc tcagtcaact ggtcttcggt
     1261 cccgccgggt tgcttgtcag gtcggttcag aatcgttatg tgggtgaaag tgaggcctct
     1321 caccgtctct tgttaaagca gttggctccc gtctcagacc agaaaggtgc actggaactt
     1381 gggtcctatc tggattttaa ccttctcttg ccccggcgtc agactatcgt tgtaccgcct
     1441 tccctgagag ccggtctgtc atttgcgctg gtcctgggca agagcggcaa gggcaagacc
     1501 aggttattgc ggcgcctgat caggcaggat cgggtaaacg gcagtatctt tgttctggac
     1561 acgcagggga cactatttga tggtctgcag tcggagtttt cgcacgcgga tactgtctgt
     1621 ttttttgatt tctccgctac cgccccggag ctgactgctt cggcgcactc cccatccgct
     1681 tccagagagc agctcagcca gcttgccgcc cagtgcatgg ttgaggctct cggtggcgtc
     1741 tcgttaatgc aaaaggtaaa catgcccggc ttgtcgaaag ataaggaaat ggctcttact
     1801 gaggtcctgg agggtttctt ccttgagaag atcaaggtgg atgaacttcc ttctcgactg
     1861 caagcttcag gtgttggcag tgagtgttct gcccttatct tgtcgcttct gaccttgccg
     1921 tgcttcgcct gcctacccgc cctgcttccc ggctgtgtca gggcctttga gaccggcgg
//
LOCUS       DAOQNZ010000818         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38877_length_1976_cov_3.858928, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000818 DAOQNZ010000000
VERSION     DAOQNZ010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38877_length_1976_cov_3.858928"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKW73_13695"
     CDS             <1..866
                     /locus_tag="PKW73_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNA74393.1"
                     /translation="LYLCNAEQEATEKLKSQPQFFPSDNLEVRQFEARSKDGTMVPYF
                     LVAEKNLELNGQNPTLLYGYGGFEVAMTPGYRALTGSAWLAKGGVYVLANIMGGGEFG
                     PTWHEAARKENRQKAYDDFIAVAEDLIKRKITTKEHLGIEGGSNGGLLMGVMFTQRPD
                     LFGAVVCGSPLLDMKRYKHLLAGASWMDEYGDPDKPEEWAYISKYSPYQNLKAGAEYP
                     PILITTSTLDDRVHPGHARKFAARLKELGHQVWYYENIEGGHSAAANNKQTAYMKALS
                     FSFMWKALKGK"
     gene            1019..1852
                     /locus_tag="PKW73_13700"
     CDS             1019..1852
                     /locus_tag="PKW73_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74394.1"
                     /translation="MPTPAVLLLLTAAPGLAALLLLTAAPGLSAKPLEAQQASQTNLK
                     LNPGEAFLIERNSRYGTESIYVNQNGMRIIHPHLQLDIIAKPPNWNLIWCSGKSQGYF
                     EEPLREFRMRRGMTAVAGRPPNWPAVGTTYAGLKAKKLLPPISSKEQATGLPFMSRVE
                     GTRPQYVKRAEIYMTEAIKLPQGAARILAAEHCLPQFDSFPLACHEILSDGTVQKPWS
                     TKSIKAVKEPAGIIQIPSRYHLCKSQDEAVNSGFVSDVDEMVRDLKIGEDFGSGKKSG
                     K"
BASE COUNT          513 a          549 c          532 g          382 t
ORIGIN      
        1 ccctctatct ttgcaatgcg gagcaagagg caacggaaaa gctcaagagc caaccgcaat
       61 tcttccccag cgacaatctg gaggtaagac aatttgaggc ccgctcaaaa gacggcacca
      121 tggtcccata ctttctggtg gcggaaaaga accttgagct gaacggccaa aacccgaccc
      181 tgctctacgg ctacggcggc ttcgaggtag ccatgacacc gggctaccgg gccctgaccg
      241 gaagcgcctg gctggccaaa gggggcgtct atgtcctggc taacatcatg ggtggcggcg
      301 aattcggacc cacctggcat gaagcggcgc gcaaagagaa tcgccagaaa gcttacgatg
      361 atttcattgc cgtagccgaa gacctgatca aaagaaaaat taccacaaaa gagcaccttg
      421 gtatagaagg aggctccaac ggcggcttac tgatgggggt gatgtttacc cagagacccg
      481 atcttttcgg agccgtggtg tgcggttcgc cgctcctcga tatgaagcga tacaagcatt
      541 tgctggcggg agccagttgg atggacgaat acggcgatcc ggataagccg gaagaatggg
      601 cctatataag caagtattcg ccttatcaga acctcaaagc gggagctgag tatccaccga
      661 ttctcattac cacgtccact ctggacgacc gggtgcaccc ggggcatgcc cgcaaatttg
      721 ccgcccgcct caaagaactc gggcaccagg tctggtacta cgagaatatt gaaggcggtc
      781 atagcgcagc cgccaataac aagcaaaccg cctatatgaa ggcactgtct tttagcttta
      841 tgtggaaggc cttgaaaggc aagtaaggcc gccacatata tcttgcctgc tttttatggt
      901 atgactgaag tggagactcc gctgcagcta tgctacgggc aacgcctgaa aaaaaatgaa
      961 atggagctgt cttaacctac ccagagcggt gagccgtctc aattcaaaca ggcggaggat
     1021 gccgacgccg gcagttctgc tcttgctcac ggccgcaccg ggactggcag ctttgctctt
     1081 gctcacggct gcgccggggc tttccgccaa gccgctggag gctcagcagg caagccaaac
     1141 caacttgaag cttaacccgg gagaagcgtt cctgatcgag cgcaacagtc gctacggcac
     1201 agaatccata tacgtcaacc agaacggcat gcggatcatt catccccact tgcagctcga
     1261 cattatagcc aaaccgccca actggaatct gatctggtgc agcggcaaat ctcagggtta
     1321 cttcgaggaa ccactgcgag aatttcgcat gcgccggggc atgaccgccg tagccggccg
     1381 cccgcccaac tggccggccg tcggcaccac ttacgccggt ctgaaagcca aaaagctcct
     1441 gccgccaata tcatcaaaag agcaggccac cggccttcct ttcatgagcc gggtggaagg
     1501 aacccgccct caatacgtta aacgtgccga gatttatatg accgaggcca ttaaactgcc
     1561 tcagggcgca gccagaatcc tggccgccga gcactgcttg ccgcaatttg acagctttcc
     1621 cctggcctgc catgaaattt taagtgatgg aaccgtgcaa aaaccctggt cgactaagag
     1681 cataaaagcg gtgaaagaac ccgccggcat aattcagatt cccagccgct accatctctg
     1741 caaaagtcag gatgaagcag tcaattcagg cttcgtaagc gacgtagacg aaatggtgag
     1801 ggacctgaaa atcggcgaag acttcggcag cgggaagaaa tcaggtaaat aactagcttt
     1861 tatccgaggc ggcaaaagca atctttttct ggctaaaagc cttgtgcaca cccttattga
     1921 tctcactgcg cagggaactg ggcttggaga gatagtcgtg cgtatagact cgcagg
//
LOCUS       DAOQNZ010000819         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38883_length_1976_cov_3.309735, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000819 DAOQNZ010000000
VERSION     DAOQNZ010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38883_length_1976_cov_3.309735"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PKW73_13705"
     CDS             complement(<1..1241)
                     /locus_tag="PKW73_13705"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA74395.1"
                     /translation="MIGKKNRRTPIWLTLAAVILSSCSLLDSSSKQTPLAMMAGAKPL
                     KLFAAYKSPPRKPETKPLPAPAPDPANIDLSKPANVTVTLSAGAAGNITVAPVGTRSL
                     KQALRFSSTVESSNPGTASVTSMVHGIITRVLVDVGDTVKAGQVVAYINCPELSDAQS
                     SFVERKARVMEAAANVDLIKTRVSLAEADAARLKTLFGEGISAKKDVEAGQSRIASTK
                     AELETAKAMLAASQIQFTAARSRLVSLGFDPQSMEESKLTTELALRSPISGQVSQRNA
                     QAGQTVGASGSPAATVLFTIVDLSRVWVMLEVPQSEVSKIKLNAPVTFTTEVAPGQKF
                     IGRVTRLGERFDPQSRTASVRTEIENPRAILKPGLLVLAEVATSAAPQDKLSIPNAAI
                     QRIGGQDLVFKKTGPQSYRACR"
     gene            complement(1356..1814)
                     /locus_tag="PKW73_13710"
     CDS             complement(1356..1814)
                     /locus_tag="PKW73_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74396.1"
                     /translation="MSKSKLNLHLSSYKRIILSVLVFTLMWTGTLLHDQLGLELSKLT
                     SAQNFLNSSKGNDVLSLLSTPERCPCAAHTHSPLISAAVDETDSHKNVGSDKDSHNHF
                     QICRHALLATISHFICALSSKALPPATPETVALLAAESRPIYILKRALLI"
BASE COUNT          411 a          479 c          594 g          492 t
ORIGIN      
        1 cggcaggcgc ggtagctctg cggccccgtt ttcttgaaga ccagatcctg tccgccgatg
       61 cgttgaatgg cggcgttggg aatgctcagt ttgtcctgag gggcggcgct ggtggctact
      121 tccgccagaa ccagaagacc gggtttgaga atagccctgg ggttttcaat ttccgttcgc
      181 accgaggcgg tgcggctctg ggggtcaaag cgctcgccca ggcgggtgac cctgccgatg
      241 aacttttgac cgggagccac ttcggtggtg aaggtgacgg gagcattcag cttgattttt
      301 gaaacttccg actgcggtac ttccagcatc acccagacgc gggagagatc gacgatggtg
      361 aaaagcaccg tcgcggcagg gctgcctgag gcgcctacag tctgaccggc ctgggcgttt
      421 ctctggctta cctgtccgga gatgggtgag cgcagggcaa gctcggtggt gagcttgctc
      481 tcttccatgg actgcgggtc aaaacccaag ctgacaaggc gggagcgggc tgccgtaaac
      541 tgaatctggc tggcggcaag catggcttta gccgtttcca gttcggcctt ggtgctggct
      601 atacgcgatt gtccggcttc cacatctttt ttggcggaga tgccctcgcc aaatagggtc
      661 ttgagcctgg ctgcgtctgc ctcagccaga cttactctgg tttttatgag gtccacattg
      721 gcggctgctt ccatcaccct ggccttgcgc tcaacgaagc tggattgggc atcggaaagc
      781 tccgggcagt ttatataggc caccacctga ccggctttga cggtatcgcc cacatctacc
      841 aggacccggg tgatgatgcc gtgtaccata gatgttaccg atgccgtgcc gggattgctg
      901 gactccaccg tggaggagaa gcgcagggct tgcttgaggc tcctggtgcc gaccggggcc
      961 acagtgatat taccggccgc ccccgcactg agggtgacgg tgacattagc gggtttgctc
     1021 aaatcgatat tggccggatc tggagccggt gccggcaggg gcttggtttc cggtttcctg
     1081 ggggggcttt tgtaggcggc aaaaagcttt agtggcttgg caccggccat catagccagt
     1141 ggagtttgtt tggatgagga gtcaagtaaa gagcaactgc tcaatatgac tgccgccaat
     1201 gtcagccaga tcggagtccg acggtttttc tttccaatca tgatgatact tcttatagag
     1261 gttcgaaagt ttttgctttc ggctaaaact atatgctgca ctaaaccagg ttgagccctg
     1321 ggttcttagt gcaagcctgg gaaccaagaa ggaatctaaa ttaagagggc gcgcttcaat
     1381 atatagattg gccggctctc cgcagctagc agggcaaccg tttcgggagt agccggcggt
     1441 aatgctttac tgctcagggc acagataaaa tgagagatgg tggccagtaa ggcgtgccgg
     1501 caaatctgaa aatgattgtg gctatcttta tcgctgccga cattcttatg gctgtcggtt
     1561 tcgtcgacag cggcggagat cagggggctg tgagtgtgag ccgcacaggg gcaacgctca
     1621 ggtgtgctga gcagactgag aacatcgttg cctttagaac tatttaaaaa gttttgggcg
     1681 ctcgttagtt ttgagagttc aaggccgagc tggtcgtgta agagcgtgcc tgtccacatc
     1741 agcgtgaata ccagcactga caggatgatt cgtttgtaac tgcttaagtg caggtttagt
     1801 ttcgatttgc tcaagagaaa gtcctcgaat tcgaatctac tctctgcctg taatacttac
     1861 caaatacata ataccagagc ttgcctcttt taaacagttt atgttgtcga agatgtacta
     1921 tttatcggcg ttttgataga aattctcaat aagagcagac ctggattcgt tgacga
//
LOCUS       DAOQNZ010000820         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38923_length_1975_cov_3.363542, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000820 DAOQNZ010000000
VERSION     DAOQNZ010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38923_length_1975_cov_3.363542"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..749)
                     /locus_tag="PKW73_13715"
     CDS             complement(168..749)
                     /locus_tag="PKW73_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNA74397.1"
                     /translation="MDELTVVPDVLFRELVTTTSPSPVLAAAAMPVVTIEMLLARQAA
                     TLVLCENLQDPGNLGTIIRSALAFDAGALILSHGSVDAFNPKVVRSAMGGLFQLPILS
                     IDLEIACRHLKEAGYIFLAFSPEAKITTADYPFTEEKVILLVGNEGNGLTERAEALAD
                     VKISIPQNPAVESLNVAVATSIALYARAMAQKR"
     gene            complement(986..1342)
                     /gene="rplT"
                     /locus_tag="PKW73_13720"
     CDS             complement(986..1342)
                     /gene="rplT"
                     /locus_tag="PKW73_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002507379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNA74398.1"
                     /translation="MARVKRGNVLQKRHKKILKYAKGFRGSKSRLFIAANQALMQAWK
                     NAYRDRKKRKRDFRSLWITRINAAARTHGLSYSRMIDGLSKANIQVNRKMLADLAVRD
                     KEAFATLAKKALAAVK"
     gene            complement(1471..1680)
                     /gene="rpmI"
                     /locus_tag="PKW73_13725"
     CDS             complement(1471..1680)
                     /gene="rpmI"
                     /locus_tag="PKW73_13725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNA74399.1"
                     /translation="MPKMKTNKAAARRFKITGTGKILRRQAGKRHLNEWMKANKKRSL
                     RGYAAVHPDVEQHVLEMFPYRKYVR"
BASE COUNT          409 a          506 c          527 g          533 t
ORIGIN      
        1 acctggcgtt gtccggcaaa actctttctg gagcctttgc cgatatctcg tcttcttttg
       61 tttcgccaga ttgcaagctg ggtgttcctt ctctcaagta atttctctca agtaatcaat
      121 gcgagtttct cgggatgttt tagaaaacgg tcaacccgtc cctcatttta acgcttttga
      181 gccattgctc tggcgtaaag agcgattgag gtggcaaccg ctacattcag ggattccacc
      241 gccgggttct ggggaatgct gatcttgacg tcggccaggg cttccgcccg ttccgtcaga
      301 ccgtttcctt cattacccac cagcaaaatc actttttctt cggtgaaggg atagtcggct
      361 gttgttattt ttgcttcagg ggagaaggcc aggaaaatat agcctgcttc tttcaagtgg
      421 cggcaggcga tttccaggtc tatgctcagt atgggcagct gaaatagccc gcccatggca
      481 cttcgcacca ccttggggtt aaaggcatcg acggaaccat gggaaagaat aagggcaccg
      541 gcatcaaaag ccagggctga gcgaataata gttcctaaat tgccggggtc ttgcagattt
      601 tcacacagta ccagggtggc cgcttgcctg gccagcagca tctcgatcgt caccacaggc
      661 atggctgccg ctgccaggac cggactggga ctggtagttg tgaccagttc ccgaaagagg
      721 acgtccggca ccacggtaag ctcatccatg agggctggac ggggcgagag ttccttcaga
      781 agagcttcac tcagccccag gtcgaaatac tcctgcgtga ccagcagatg ctcgacccgt
      841 aagcccttac tcagggcttc cagagtgagc ttctcgcctt caacaagaaa aagcccggtt
      901 ttttgccggg cctgtcgctc tttcagcgac cttattttct tgagcaggtt gttgctcgat
      961 gaggtgattc tcttcggatt cagaattact tgaccgctgc gagggccttt ttggccaggg
     1021 tagcgaaagc ttctttgtcg cgaacagcga ggtcagccag cattttacgg ttgacctgga
     1081 tgttggcttt agacagaccg tcaatcatgc ggctgtagct caagccatgg gtgcgagcag
     1141 cagcgttgat acgggtgatc cagaggctac ggaagtcacg cttgcgcttc ttccggtcac
     1201 ggtaagcatt tttccaggcc tgcatgaggg cctgattggc ggctatgaaa agccttgatt
     1261 tggaaccacg aaaacccttg gcgtatttga ggattttctt gtggcgtttc tggaggacat
     1321 tgcctctctt gactcttgcc atcttattct ccttcttgga ctctttttgt tgtttgtcgt
     1381 atttcgactg ccggccggcg gagaggagtc attcccttag gcctggctat tgaacgaaat
     1441 gtcgatgaat ttacgctgcc aaccggttgg ttagcgaacg tacttccggt aagggaacat
     1501 ttccaacacg tgttgctcaa cgtcgggatg cacggcggca tagcctctca aagagcgctt
     1561 cttgttggct ttcatccatt cgttcaagtg gcgcttgcct gcctgtctgc gcaaaatctt
     1621 gccggtgccg gtgattttga agcgacgggc agcagcttta ttggttttca tttttggcat
     1681 aattttttcc actaaagcga actgttgcta tctgcggttt ccaaagaaac gcagccggcc
     1741 ctccagatag agagcacagg gttccaataa tagcaccaca atccgggact tttggggcta
     1801 tctcaaggtt tgctcaatat ttcgccactt tccgggatga ctgtacggca attgtgcccg
     1861 tgttcgcagc ggtaagcggg agattacatg acactaagct cctggcccga gtgtaattca
     1921 acttaacctt accggcccgg catggtcttg ccctggctca agggttgtgt caagc
//
LOCUS       DAOQNZ010000821         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38925_length_1975_cov_3.222917, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000821 DAOQNZ010000000
VERSION     DAOQNZ010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38925_length_1975_cov_3.222917"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..446)
                     /locus_tag="PKW73_13730"
     CDS             complement(252..446)
                     /locus_tag="PKW73_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74400.1"
                     /translation="MKLPIWIILFLTFLMAKGASAAETARAIGWIPTWQQGLEEARLS
                     GKPIMLYAGAPSCSGVPGIW"
     gene            662..>1975
                     /locus_tag="PKW73_13735"
     CDS             662..>1975
                     /locus_tag="PKW73_13735"
                     /inference="COORDINATES: protein motif:HMM:NF025746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4419 domain-containing protein"
                     /protein_id="HNA74401.1"
                     /translation="MAVSRLGNSVTFQVDDVEPVTAPVALWKTHEGINALLDAVASQP
                     GERILSCSGYNSSCMRAITYHPVIAAVHTAFAEHRPLCLSPDIIWTTILQGFALHVRN
                     NHEALRSRLVSHNGRKTLMVTFDGYFPGSPENDWSGMVDAMAGALRGEVGTIYDALVA
                     DFSTTGDLERTVSALTILDAFEPYFEYQMLCICGIPSITLEGTSADWQRLRQKVECLA
                     AFDLDWWLSSLRPILDQFERASRGDIDLNHWQHIYKNRKAYGWDKMNGWITRLVPYVR
                     DHGTGAYSVRNPMFAVDLYWEPKLQPSVNGIDPGSFHGHAEGPSINSHDLPSGLSSVP
                     FKVVCVDRTDYIQLLGGFVGIEQNGEGALRPKLGLAVRREAGLGKALQSVPEGCRLLP
                     PLSPADFSEGYRRLAGADPFEPTTVPGEMNTFYLRHDGIEFSNPNA"
BASE COUNT          408 a          493 c          570 g          504 t
ORIGIN      
        1 agagccattt cgcgagggtc attgaagacg aagccggggc tgcggatgcc gggcgtcagc
       61 tgcctgccgt aggggtctaa gaatgtgaaa agggtatttt ccaggtcggc accggggcgc
      121 cagtatgacc ttaaaacttt ggcttcgctc tcgctttcat aggtcatgag tctgatgcag
      181 acaaagtttc gggaggcgtc aataacctcg ggctgagaca ggtaactccc gtctgtacgt
      241 tgtttgcccg gtcaccagat gcccggcacg ccgctgcaac tgggtgcgcc ggcatagagc
      301 atgatgggct taccgctcag cctggcttct tccagtccct gttgccaggt tggtatccag
      361 ccgatggccc gggccgtttc ggcggcggaa gcacctttgg ccatgaggaa ggtgagaaag
      421 agaatgatcc agattggcaa tttcatggtt ggaaaactcc tctgaattgt aacgacggcc
      481 tttaccggcg acgaagttgt ctatgtagag tagtttgcca ggcgaatatg gagaaattat
      541 ggattaaccc acccctgcag gaagcccgac gactgcttaa actttcgatg tggctgggcc
      601 cttaagatta cagatggcgg ttgcgggtat attgcctttg ctctggcagg gagggtggct
      661 tgtggctgta tccagactgg gaaactcggt aacctttcag gttgatgatg ttgagccggt
      721 gacagcacca gtggctctct ggaagaccca tgaaggcatt aatgctttgc tcgatgcagt
      781 agcgtcccag ccgggggagc gcatcttatc gtgctccggc tacaattcca gctgcatgcg
      841 ggcgataaca tatcacccgg ttattgccgc cgtacatacg gcctttgccg agcatcgtcc
      901 gctctgtctt tcgcccgata ttatctggac tacaatattg cagggcttcg ctctgcatgt
      961 gcgcaataat catgaggcgt tacgctctcg tctggtctca cacaacggtc gcaaaacgct
     1021 gatggtgact tttgacggct atttcccagg ctctcccgag aatgattggt cgggcatggt
     1081 cgacgctatg gcaggcgctc tgagaggcga agtaggcacc atatacgatg cactggtggc
     1141 ggatttctcc actaccggcg acctcgagcg aactgtcagc gccctcacta ttcttgatgc
     1201 ctttgagccc tattttgagt atcagatgtt gtgcatctgc ggtattccgt ccattaccct
     1261 ggagggtacc agtgccgatt ggcagcggct tcggcagaag gtagagtgtc tggctgcttt
     1321 cgatcttgat tggtggctca gttctttgcg tccgattctt gatcagttcg agcgggcttc
     1381 caggggtgat atcgatctaa accactggca gcatatttac aaaaatcgca aggcttatgg
     1441 ttgggataaa atgaacggct ggatcaccag gctagtgccc tatgttcgcg atcatggtac
     1501 aggtgcatat agcgttcgca atcctatgtt tgccgtggat ctatactggg agccgaagtt
     1561 gcagccgagt gtcaacggaa ttgatcctgg ttccttccac ggccacgcgg agggcccttc
     1621 aataaatagc cacgatttac cctccggttt gtcttccgtg ccttttaaag tggtttgtgt
     1681 ggatagaacc gattatattc agttgctagg cggttttgtg ggtatcgagc aaaacggcga
     1741 gggtgccttg cgtcccaaac ttggtctggc tgtaagacga gaggcggggc tcggcaaggc
     1801 actgcagtca gtacctgaag ggtgccgcct cttgccgccc ctttcgccag ctgacttcag
     1861 tgaaggttac agaagactgg caggcgcgga tccttttgag ccgactaccg ttcctggtga
     1921 aatgaatacg ttttatttgc gccacgacgg tatagagttt tccaacccta atgcc
//
LOCUS       DAOQNZ010000822         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38934_length_1975_cov_2.837500, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000822 DAOQNZ010000000
VERSION     DAOQNZ010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38934_length_1975_cov_2.837500"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /gene="msrA"
                     /locus_tag="PKW73_13740"
     CDS             <1..370
                     /gene="msrA"
                     /locus_tag="PKW73_13740"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNA74402.1"
                     /translation="SYEDVCSHGTGHAEAVRVVYNPKQISFATLTKEFLALHDPTTMN
                     RQGPDVGDQYRSAIFYTNDSQLKEAKTVIGKEQNFMGFKKIVTSLEPFNKFYTAEEYH
                     QDYFAKHPGQGCHYRPPKAK"
     gene            408..1061
                     /locus_tag="PKW73_13745"
     CDS             408..1061
                     /locus_tag="PKW73_13745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA74403.1"
                     /translation="MIFGTKKSNKAIVESWVKTYSREFYQYAYLRLKRHEDAEDVVQL
                     TFIKAFRAFNTFKTGTSEKAWLYAILSNNLKDHLRRISNKPQEANIDEEEDLENLLVD
                     TRDGPETEASRKMEHERLAMGIANLPEQFAAPLLMREVSDMSYKDIAEYLSVPIGTVM
                     SRLSRARKALYEILTSPASPKPFKAEADSNSESVKETPEEDTSSKGIMPNKEGRLSQ"
     gene            1058..1882
                     /locus_tag="PKW73_13750"
     CDS             1058..1882
                     /locus_tag="PKW73_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNA74404.1"
                     /translation="MNCDETKALIGPAVDDELTSADTARLMSHLEQCRSCKQEWDKLM
                     AVREGIKSITQAEPAPADLEKRLLNAIHLEHQRNIRLRAGFLLVAAAAVAAFGGGAFL
                     LFAPGTTPKLATGNVETTPRAISSIEKLLAAAGHHSENSDDAAITIDFVGNKDAKDLS
                     PLAGFAVKQSTLASSHGTFKLSGSDMLKVGKQNMVRLCYTSDDDARVHCIDCYQAPQG
                     MLSFDGAKAQTINGKTVFSGRIGDDTILKISENGVDVFYVTPLPIEELQDLIKANV"
BASE COUNT          590 a          533 c          473 g          379 t
ORIGIN      
        1 cagctatgaa gacgtctgca gccacggtac aggccacgca gaagcagtgc gagtcgtcta
       61 caaccccaaa caaattagct tcgccaccct caccaaagaa ttcctggccc tgcacgatcc
      121 aaccacaatg aacaggcagg ggccggacgt gggggatcaa taccgttcag ccatatttta
      181 tacaaacgat agccagctca aagaagccaa aacagtgatc ggcaaagagc aaaacttcat
      241 gggcttcaag aaaattgtga ccagcctgga gcccttcaac aaattctaca cagcggagga
      301 atatcaccag gactactttg ccaaacatcc cggtcagggc tgccactaca gaccgccgaa
      361 agcaaaataa gagctaagat gtcgcaggca agaaagacaa acaaccaatg atcttcggca
      421 caaaaaaatc gaataaggcg atcgtagagt cctgggtaaa gacttattcc cgggaattct
      481 atcagtacgc ttatctgcga ctgaagcgcc atgaggatgc ggaagatgtg gtgcaactca
      541 ccttcatcaa ggcctttcga gccttcaaca cctttaaaac aggaactagc gagaaagcct
      601 ggctgtacgc catcctcagc aacaatctca aagatcactt gcggcgcatc agcaacaaac
      661 cccaggaagc caacattgat gaggaggagg acctggaaaa tctactggtt gacaccagag
      721 atggtccgga aacagaagcg agcagaaaaa tggagcacga gagactggcc atgggcatag
      781 ccaatcttcc tgaacagttc gccgcccctc ttttgatgag agaggtgagt gacatgtcct
      841 acaaggacat cgccgaatat ctctcagtgc caatcggcac cgtcatgtcg agactatcga
      901 gagcacgcaa ggctctctat gagattctca ccagcccggc ttctccaaaa ccattcaagg
      961 ctgaggcaga ctcaaactcc gaatcagtga aagagacccc agaagaagat acaagctcaa
     1021 aaggtattat gccgaacaaa gaagggaggt tgagccaatg aattgcgacg agactaaagc
     1081 gctcatcggc cctgctgttg acgatgaatt gaccagcgcc gatactgcca gattaatgag
     1141 tcaccttgaa caatgccgta gctgcaaaca ggaatgggat aagctcatgg ctgtaagaga
     1201 aggtataaag agtattacac aggccgagcc ggcgccggcc gatctggaaa agcgccttct
     1261 caatgccatt catcttgaac accaacgcaa catacgtttg agagccggct tccttttggt
     1321 agcggcggca gccgtagcag ctttcggcgg cggcgccttt ctcttatttg ccccgggcac
     1381 aacaccgaaa ttagcaacgg gcaatgtcga aaccaccccc agagccataa gcagtattga
     1441 aaagttgctt gcggccgccg ggcaccattc tgaaaattcg gacgatgccg ccataaccat
     1501 cgacttcgtc ggaaacaaag acgcaaaaga cctcagcccg ctggccggtt ttgccgtaaa
     1561 acaaagcact cttgcctcaa gtcacggcac tttcaaactt tccggcagcg acatgctcaa
     1621 agtagggaag cagaatatgg tcaggctctg ctataccagc gacgatgatg caagggtcca
     1681 ctgcattgac tgctaccagg ctccgcaagg aatgctctcc tttgacggcg ccaaggctca
     1741 gacaatcaac ggcaaaaccg tattttcagg acgcattggt gatgacacca tattgaaaat
     1801 ttcggaaaac ggcgtcgatg ttttctatgt cacacctctt cccatcgaag aattgcaaga
     1861 cctgatcaag gcaaatgtgt aagcgaaact agttttgctg ctgacgccac tggcgttaag
     1921 catctctgcc gcacacactt tgcctttgca ccgccgccca cagcgaaggt cagac
//
LOCUS       DAOQNZ010000823         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38950_length_1974_cov_4.176133, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000823 DAOQNZ010000000
VERSION     DAOQNZ010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38950_length_1974_cov_4.176133"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKW73_13755"
     CDS             <1..987
                     /locus_tag="PKW73_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA74405.1"
                     /translation="TNPALATLLAYKEPPVKRNIRDLVYFSDTTGKGTTLRLPTSEEE
                     RPWCEAEAIARDGRTVAVELGMRLIDTHDGSRVLVILVDIRQRREIERLKQEFLSMVS
                     HDLRTPLNSFQAFLELVADGIYGELNEAGAKKVSLLESEVERLMRLIQQLLELDRLES
                     GTVSLSLQSHDGASLMRRAVNSVRSLAERRSISLDILETTVAVKADSDKIVQVIVNLL
                     SNAIKFSPDGAVISLSVARHGNLATFKVTDRGRGIPANVQERIFERFKQVKATDASEK
                     GGAGLGLAICKNIVEQHGGKIGVESQEGAGSTFWFKIPIASGGAQSSQESGS"
     gene            complement(984..1679)
                     /locus_tag="PKW73_13760"
     CDS             complement(984..1679)
                     /locus_tag="PKW73_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA74406.1"
                     /translation="MAKILLVEDDALFAQMIVDYLTAQQFHVEMTDNGKDAQALLRDY
                     SFDVIILDWGLPDMTGIDLLRDFRNRGSMTPVLMLTGRDKVEEKEQGLDSGADDYLTK
                     PFNMRELTARVRALIRRTGPSPTGILKAGHISLNPSTHVVTKNGEEVRLLPKEFSLLE
                     YLMRHPSQVFTAEMLLDRVWHSESDAGTEAVRTCLKRLRKKIDDDDNESLIQTLHGVG
                     YKINPKFAGGESL"
     gene            complement(1767..>1974)
                     /locus_tag="PKW73_13765"
     CDS             complement(1767..>1974)
                     /locus_tag="PKW73_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74407.1"
                     /translation="SLSRTFTIPVPSSSLKAGAAQANSLELPINIKSTDLESGVYQQV
                     RVRAVDGQGRTLGQPSAPVILRLN"
BASE COUNT          421 a          501 c          556 g          496 t
ORIGIN      
        1 accaatccgg cgctggccac tttgcttgcc tacaaagaac cgcctgtgaa gcgcaatatt
       61 cgtgatctgg tctatttcag tgatactacc ggtaagggca ctaccttgcg tctgccgacc
      121 tcggaagaag agagaccctg gtgcgaggcc gaggctattg ccagggatgg acgcactgtt
      181 gccgttgagc ttggcatgcg tctgatcgac acccatgacg gtagcagggt gctggttatt
      241 ctggtggata ttcggcagcg ccgcgaaatt gagcgcttga aacaagaatt cctttccatg
      301 gtcagccacg acctgcgtac gcctctcaat tcctttcaag ccttcctgga gcttgtagcc
      361 gatggtatat acggtgagct gaatgaggcg ggagccaaaa aagtaagtct tctggagagt
      421 gaagtggagc gcttgatgcg ccttatccaa cagcttcttg aactggaccg gcttgaatcg
      481 ggcacggtca gcctgtcctt gcagtcccac gatggtgcct ccctcatgcg tcgggcggtg
      541 aactcggtgc gtagtctggc cgagcggcgt tccatcagcc tggatatact ggagacgacg
      601 gtggcggtga aggcggatag cgataagatt gtccaggtta ttgtcaattt gctctccaac
      661 gccatcaagt tttcccctga tggtgctgtc ataagtttat ccgtagccag gcacggcaac
      721 ctggctactt tcaaggttac agaccgcggt cgaggcattc cggccaatgt gcaggagcgt
      781 attttcgagc gcttcaagca agtgaaagcc accgatgcca gtgagaaagg cggcgccggg
      841 cttgggctgg ccatctgcaa gaatattgtc gagcagcacg ggggcaagat tggtgtggag
      901 agccaggaag gtgcaggcag caccttctgg tttaaaattc cgattgcatc cggtggagct
      961 caaagcagcc aggaaagtgg ctcttaaagg ctttcaccgc ctgcgaattt aggatttatt
     1021 ttgtagccga ctccatgcag agtctgaatg agagactcgt tatcatcgtc atcgattttc
     1081 ttgcgcagtc tcttgaggca ggttctcact gcttccgtgc cggcatcgga ttccgaatgc
     1141 cagactcgat ctaaaagcat ttcggcagtg aatacctggc tcgggtggcg catcaaatat
     1201 tccagcaggg aaaattcttt tggcaggagt cttacctctt ccccgttttt ggtcaccacg
     1261 tgggtagaag gattgaggct gatatgaccg gctttgagaa tgcctgtggg gctcggaccg
     1321 gtgcggcgga tcaaggctct cactcgggct gtcaattcgc gcatgttgaa gggtttggtg
     1381 agatagtcat cggcacctga atcaagtccc tgttctttct cttctacttt gtcgcggcct
     1441 gtgagcataa gcaccggcgt catactgccg cgatttctaa agtcgcgcaa gaggtctatt
     1501 ccggtcatgt cgggcagacc ccagtccagg atgattacat caaaactgta atcgcgtaag
     1561 agtgcctgcg cgtcttttcc gttgtcggtc atttctacat ggaactgctg agcggtcaga
     1621 tagtccacga tcatctgggc aaagagcgca tcgtcttcga ctagaagaat tttggccatt
     1681 aatttgctgc tggtacttta catacgggga acgaacttat tctcgcacaa attttcccat
     1741 aaatttaatg cccgctcagg cttctcttag ttcagtcgca ggatgacagg agctgaaggt
     1801 tgccccaggg tacgcccttg cccgtccacg gccctcactc gtacttgctg gtagacgccg
     1861 ctttccaggt cggtgctctt gatatttatc ggtaactcca gagagtttgc ttgcgccgcc
     1921 ccggccttca ggctcgaact cgggaccgga atcgtgaaag tgcggctgag tgaa
//
LOCUS       DAOQNZ010000824         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_38990_length_1973_cov_4.031283, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000824 DAOQNZ010000000
VERSION     DAOQNZ010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38990_length_1973_cov_4.031283"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1599)
                     /locus_tag="PKW73_13770"
     CDS             complement(127..1599)
                     /locus_tag="PKW73_13770"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNA74408.1"
                     /translation="MPIISYHVLSYLIFLIAALSQSEFPPALSLTYEECLDYLESIGP
                     TFERPGLERMESFFACQDHPQPQNKIAAVHVGGTNGKGSVTTFIASILEAFGYRTGKF
                     TGPHLKRFNERFVIDGRLISDEDFAKTLTAVREASLRFGDEHPEHGSLTWFELLMAMS
                     VSYFNDAGVQACVYEVGLGGRFDATNVLQNVLVSVLTNVELDHVHILGDTREKIAFEK
                     AGIIKPAVPLITAVQEPALRVLKERALTLNAPLLAISGATYDSQFLRDFELTIQDPSG
                     MFTREQLQSIEFVERSLREGVIVELGLKGAYQRSNAMVAVFAAVNYLVLREKRSFGQD
                     DLRSLLAGLKKAYWPGRFQILAEPPAILDGAHNGHGALALRRSLDELYPGRKLVFIFA
                     CFANKDHEEILQNLLRAGDRVYLPHLSNTRAIRSALELEVLANKLGAKAEVTATFQEA
                     SLKGQEFLRENAGAYGERLIITGSFALIKEALPLKADPLN"
     gene            1752..>1973
                     /locus_tag="PKW73_13775"
     CDS             1752..>1973
                     /locus_tag="PKW73_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74409.1"
                     /translation="MPIDTPTAATAADALPELSFNAGSSDQSLFLRVMDEVRPARAGE
                     AREKGSESESQLQVPRPGETSIEKITLPDG"
BASE COUNT          512 a          530 c          511 g          420 t
ORIGIN      
        1 ccggtcagct ctcgctgaga ccgactgttg gctggctgac ctgaactgga agacaatgcc
       61 gcttatgctc ctgtggtgag ctatatttta gcagcttgaa ccaggtgcag aatatttctc
      121 ggcgcttcag ttcaagggat cggccttcag gggcagagcc tccttaatga gggcaaaaga
      181 accggtaata atgagcctct cgccataggc tccggcattt tcccgcaaga attcctgccc
      241 tttcaggctg gcttcctgaa aagtagcagt cacctcggcc ttggccccca gtttattagc
      301 cagaacttcc agctcaaggg cactgcgaat cgccctggtg ttactcaagt gcggcaaata
      361 aaccctatcc ccggccctga gcaagttttg caaaatctcc tcgtgatctt tattggcaaa
      421 gcaagcaaaa atgaagacca gcttccggcc cggataaagt tcgtccaggg agcggcgcaa
      481 agccagagca ccatgtccat tatgggcacc gtccaggatg gcaggcggtt ctgccaggat
      541 ttgaaagcgg cctggccagt aggctttttt caaaccggca agcagtgacc tgaggtcgtc
      601 ctgtccgaag ctgcgctttt cgcgcagaac cagataattg accgcggcaa aaacggctac
      661 catggcgtta ctccgctggt aagcgccctt cagtccaagc tctacaataa cgccctcacg
      721 taaggagcgc tccacaaatt cgatgctctg aagctgttcg cgggtgaaca tgccggaggg
      781 atcctgaatg gtcaactcaa aatctcgcaa aaattgactg tcataagtag caccagatat
      841 ggccagaagc ggagcattca gggtcagggc ccgctccttc aaaaccctca gggcgggctc
      901 ctgcacggca gtaattagag gcaccgccgg ctttataatg ccggcctttt cgaaggctat
      961 tttctccctg gtatcgccga ggatatgaac atgatcgagt tccacattgg taagcactga
     1021 tacaagtaca ttctgaagca cattagtggc atcaaaacgt ccacccagac caacttcata
     1081 gacgcaagcc tgaacgccgg cgtcgttgaa ataagaaacc gacatagcca tgagcagctc
     1141 gaaccaggta agggagccat gctctggatg ctcgtcgcca aagcgcaaac tggcttccct
     1201 cacggccgtc aaagtcttag caaagtcctc atcggaaatg agtcggccat caatgacaaa
     1261 acgctcgttg aagcgcttca aatgcggacc ggtaaatttc ccagttctgt agccaaaagc
     1321 ttccaggatg ctggcaataa aggtagtgac actgcctttg ccgttggtgc cgcccacgtg
     1381 cacggcggca attttgtttt gcggctgggg atggtcctgg caggcaaaga agctttccat
     1441 tcgctcaagg ccgggacgct caaaagtagg acctattgac tcgaggtagt caaggcactc
     1501 ttcataagtg agactcaagg ctgggggaaa ctctgactga gacaaagcgg caatcaaaaa
     1561 aatgagatac gagagaacat ggtaactgat gataggcaag tgcgactcaa gcaaaccaca
     1621 attgttgcgg cagcatcgcc tgggagcggc agaaattttg ataaagcagt tttcatccgg
     1681 gtcatgagaa tgtcacaagc gctctgttta atgagtgagt tcacccccat tttcttttaa
     1741 acggaagcac catgccaatc gataccccca cagccgctac tgctgccgat gcgctgccgg
     1801 aattgagttt taacgccgga agctccgacc agtcactctt tcttcgggtt atggacgaag
     1861 ttcgcccggc cagggcaggc gaagccagag agaaaggctc agagagtgaa agccaactgc
     1921 aggtccctcg accgggcgaa acttccatag aaaagataac cctgcctgac ggc
//
LOCUS       DAOQNZ010000825         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39036_length_1972_cov_2.859677, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000825 DAOQNZ010000000
VERSION     DAOQNZ010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39036_length_1972_cov_2.859677"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKW73_13780"
     CDS             <1..703
                     /locus_tag="PKW73_13780"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNA74410.1"
                     /translation="KPAYVAGHSLGEFSALYAASAFDLSSAVKLVDKRSQLMEACPKG
                     AMSAVIGMAADKLEEITKEVSQTLGEPKVVIVANFNTKEQLVISGDPDAVAEAGSRAK
                     AGGAKVIPLPVGGAFHSPLMKAAADEFELLVKSIDIRDAAFPVVQNVDAKPETSGAAL
                     KEKLSRQMPSSVRWCETIEYMLAQGVTTFVEIGPGKALSGMVKKIDRSANVYNVSDAQ
                     TLEETLNALKQTAGV"
     gene            complement(1078..>1972)
                     /locus_tag="PKW73_13785"
     CDS             complement(1078..>1972)
                     /locus_tag="PKW73_13785"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNA74411.1"
                     /translation="FHVTKKQLELNDEFRTKGLAAILGMGAAPGMTNIMAAAGAGELD
                     EVKSIDITVGCIDFVKTSHPLQPPYAIDTILDEYTKEPMVFEDGEFRAKPPMSGEKLV
                     QLPEPVGACHAILTLHSEVLTLPKTYEHKGCTDVTFRLGLPTEFHERLKLLVDLGFGE
                     NEKIEIAEGKFTPRKVLAHLIERFPQPDQTPDDAEVVRVEVKGKRNGQPVNVTLETTV
                     LAHKKWGLSCGALYTGVPPAIVADMMLAGTIMERGAIPPEICVPHKEFFHELAKRTIV
                     MRKTVEEVLVEGKIEEKAACR"
BASE COUNT          438 a          492 c          517 g          525 t
ORIGIN      
        1 caagcctgct tacgtagccg gacacagcct cggcgaattt tcagccctct atgcggcctc
       61 ggcattcgat ttgtcgtcgg cggtgaagct tgtcgacaag cgctctcagc tgatggaagc
      121 ttgtcccaaa ggcgctatgt cggctgtgat tggcatggct gccgataagc tcgaagagat
      181 tacgaaggaa gtttcgcaaa ctctaggtga acccaaagtt gtaatcgtcg ctaatttcaa
      241 caccaaagaa caactggtta tttccggcga tcccgatgcg gtcgcagaag cgggcagccg
      301 agccaaggcc ggcggtgcca aagtaattcc tttgccggtt ggaggcgctt tccactcgcc
      361 tttgatgaaa gccgctgccg atgagtttga attactggtc aagtcaatag acatcaggga
      421 tgcagctttc ccagtagtgc aaaacgttga cgctaagccg gaaacaagtg gtgctgcttt
      481 gaaagaaaag ctctctcggc agatgcccag ttctgtccgc tggtgcgaaa ccatagaata
      541 tatgctcgct cagggcgtca ctacttttgt tgaaatcgga ccgggcaagg cgctttcggg
      601 catggtcaag aaaatcgatc gctccgccaa tgtttataac gtgtccgatg cacaaacgct
      661 tgaagagaca ctgaatgcac tgaagcaaac agcaggagtc tagcaatccc cacgaagggt
      721 gatattgcca atttgttcag aatggtacaa cggtataagc ggaccacctg tgtgtcttag
      781 ccaggcggat tcttttgctt gtcggccggg tcttttgcat gaacaagtgc ttgaaaaatc
      841 ggcgaaggcg tatttccggg cagggaatta cggagtacgc ggcgatattg gctttcgtgg
      901 ctctgctggt ggcactggta tttgcctttg cacctagtac gctctctgct gctgtctctg
      961 ccgcattttc atcagtcatc agtcaaataa acaatatgtc tgctgctgca ggatcttcgt
     1021 cctgaccata tcttctcagt gccagatgcg gtgtgccggc aatttgatgc caggctatta
     1081 gcggcaggct gctttttctt cgatcttgcc ttctaccagc acttcttcga cggtcttgcg
     1141 cataacgatg gtccgtttcg ccagctcatg gaagaactct ttgtgcggca cgcagatttc
     1201 cggagggata gctccacgtt ccataattgt tcctgcaagc atcatgtcgg caacaatagc
     1261 tggaggcact cctgtgtaga gtgcgccgca tgataaaccc catttcttgt gagcgaggac
     1321 cgtggtttcg agggtgacat tgacaggctg accgttgcgt tttcctttta cttctacacg
     1381 aacaacttcc gcgtcatcag gagtctggtc aggttgtggg aagcgctcaa tcaagtgagc
     1441 aagtactttt cgaggcgtaa atttcccttc ggctatctct atcttttcat tttctccgaa
     1501 gccaagatca acaagaagtt tcaggcgctc gtgaaattca gtcggcaagc ccagcctgaa
     1561 ggtgacgtct gtgcatcctt tgtgctcata cgttttgggc agcgtcagca cttcagagtg
     1621 cagagtcagt atggcgtggc aggcgcccac aggttccggt aactgaacaa gcttttcgcc
     1681 cgacattgga ggttttgcgc ggaattctcc atcttcgaaa accattggtt ctttggtgta
     1741 ttcgtcgaga atggtgtcga tcgcgtatgg cggttgaaga gggtgacttg tttttacaaa
     1801 gtcgatacaa ccaactgtga tatcgatcga tttcacttca tctagttcgc cggcaccggc
     1861 agcggccatt atattggtca tgcccggtgc agcacccatt cccaggatcg ctgccagtcc
     1921 tttggtgcgg aattcgtcat tcaattccag ctgctttttt gtcacgtgaa ac
//
LOCUS       DAOQNZ010000826         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39113_length_1970_cov_3.787467, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000826 DAOQNZ010000000
VERSION     DAOQNZ010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39113_length_1970_cov_3.787467"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKW73_13790"
     CDS             <1..574
                     /locus_tag="PKW73_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74412.1"
                     /translation="AFNTVWTYDIYQTYLAKNKSDAHYLWVGRAATAIGIVISVGTAY
                     LVMGFPSIMDYMQTVFSFFNAPLFATFLLGMFFKRTSPWGAFWGLVAGTGAAVIHYIL
                     CEQGKLVYSTAMAGNFHRSVAAWTVCFVVTLMISYLTKPKDVSELKGLVWGVGIEDGD
                     DLKKPPFLLRPGVLGCILLLVTIVLNLIFY"
     gene            587..883
                     /locus_tag="PKW73_13795"
     CDS             587..883
                     /locus_tag="PKW73_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74413.1"
                     /translation="MLDVRYPIGFLFLILGALLAIYGYGHPATFNTVNGPFPLNIDVP
                     WGIFMFLFGMGTVSLAKLDEVKKAEQEPASAAAAIEPEAPSSTSKTEPTGQEES"
     gene            complement(896..1654)
                     /locus_tag="PKW73_13800"
     CDS             complement(896..1654)
                     /locus_tag="PKW73_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74414.1"
                     /translation="MNKAYKQSNGSRMAISALAALCLGASLTVSATAQTTSTDNQDKP
                     SNVWSLPKYEIKVVPQTPVKAKAEPVLGPKARLLLLKKAAAEAKEAAKPDAAALADEQ
                     FSKQFSQDVSKQPRYVWSRQAVKSKEAREQEKLMNEYTREVSEKIASNLKVPSQATLV
                     ESSDKKYRYLLSFQLLKSGKIANFQVIDGIADMNTVPLPDPGESGSVVNALKAALNAA
                     VPLKTPPGSTIAPWDMLAVYDLGTGKFYTSLRPR"
     gene            complement(1673..>1970)
                     /locus_tag="PKW73_13805"
     CDS             complement(1673..>1970)
                     /locus_tag="PKW73_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74415.1"
                     /translation="AEHTLGATSMTEVSKLVFLADCLEASRPTDFTAPIWEALAANKN
                     TGLKSKINLDNALLVACDLSLAHLLEARRPIHPKTVDVRNHFLGIVRAAEKLGS"
BASE COUNT          379 a          529 c          539 g          523 t
ORIGIN      
        1 agctttcaac actgtgtgga cctacgacat ataccagacc tacctggcta agaataagtc
       61 tgacgcgcat tacctctggg tggggcgggc cgccaccgcc attggtatcg tcatttcggt
      121 gggcacggct tatctggtta tgggctttcc ttccataatg gactacatgc aaactgtgtt
      181 ctcctttttc aacgcaccgc ttttcgccac tttcctcctg gggatgttct tcaagcgcac
      241 ctctccctgg ggtgctttct ggggactggt ggccggcacc ggtgccgccg ttattcatta
      301 cattctctgc gagcagggca agttggttta ttccactgcc atggccggta atttccatcg
      361 ctccgtggcg gcctggaccg tctgcttcgt ggtcaccttg atgatttcct acctgaccaa
      421 gcccaaagat gtttccgaac taaaaggcct ggtctggggt gtgggtatag aggatggcga
      481 cgacctcaag aaaccgcctt tcctgcttcg cccgggtgtg ctcggctgca tcctgctgct
      541 agtaacaatt gtcctcaatc tgatttttta ttagggaggc caagacatgc tggacgttcg
      601 ctatccaatc ggctttctct tcctcattct gggtgccctt ctggctatct acggttacgg
      661 gcatcccgct actttcaata ctgtcaacgg accctttcct ctcaacatag atgtgccctg
      721 gggcattttc atgttccttt tcggcatggg cacggtttct ctagccaaac tcgatgaagt
      781 taagaaagct gagcaggagc cggcttcggc ggcagcagca attgagccag aagccccttc
      841 gagcacaagc aagaccgagc cgaccggtca ggaagagtct taaagagaaa ttgccctagc
      901 gcggtctgag gctggtataa aacttgcctg tgcccagatc atagacggcc agcatgtccc
      961 agggggcaat ggtgctgccc ggcggggtct tgagcggaac tgcggcattg agagccgcct
     1021 tgagggcatt gacgacactg ccggactctc ccggatcggg caggggcacg gtgttcatgt
     1081 cggcgatgcc gtcaatgacc tggaagttag caattttgcc gctcttgagt agttgaaagg
     1141 agagcagata acgatatttc ttgtcgcttg attcaacaag agtagcctgg gacggtactt
     1201 tcaggttgct ggcaatcttt tcggaaactt ccctggtgta ctcattcatg agtttttctt
     1261 gttcccgggc ttccttgctc ttcactgcct gcctggacca gacatagcgc ggttgcttgc
     1321 ttacatcctg gctgaactgt ttgctgaact gctcatcggc cagggcggcg gcatcaggct
     1381 tggctgcttc cttcgcttcg gcggcggcct tcttcaagag caaaagtctg gctttaggac
     1441 cgaggacagg ttcagccttg gccttgaccg gagtctgggg cacaaccttg atctcgtatt
     1501 taggcaggga ccatacgttg gaaggtttat cttgattatc agtcgaagtg gtctgggccg
     1561 tggcgctcac agtaagactt gcgccgaggc aaagggctgc cagtgccgag atcgccattc
     1621 ttgagccgtt tgactgtttg taagccttgt tcattttgcc gtctttcctt ttttaggacc
     1681 ctaacttttc agccgctcta actataccca ggaaatgatt cctgacatca accgtcttcg
     1741 ggtggatagg ccgccttgct tccaaaaggt gcgccaggct tagatcacag gcaacaagca
     1801 gggcattgtc gagattaatt ttgcttttca atccagtgtt cttgttggcg gccagcgcct
     1861 cccagatagg cgccgtgaag tcggtggggc ggcttgcctc caggcaatcg gccaggaaaa
     1921 ccagcttgga tacttccgtc atactggtgg cgcccagggt gtgctcggca
//
LOCUS       DAOQNZ010000827         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39193_length_1968_cov_4.336121, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000827 DAOQNZ010000000
VERSION     DAOQNZ010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39193_length_1968_cov_4.336121"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKW73_13810"
     CDS             complement(<1..256)
                     /locus_tag="PKW73_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74416.1"
                     /translation="MWPLTGCAVQLMDGMAERCALCQKQLCLRQQVLNLALGEEKHLL
                     CLSCLAADNDTGAEELLTRLAAYVRGRDCFLKEWRRYTGLK"
     gene            482..>1968
                     /locus_tag="PKW73_13815"
     CDS             482..>1968
                     /locus_tag="PKW73_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74417.1"
                     /translation="MAFTPEVAIRTSQTPTSDALDRALLDPSKVGFKAGKTSDQGREP
                     QKTENESASVRLIQELQEPGKAQKSSVATNVEGKTAAASEPGPSTETKDQLRLSGLGS
                     LTAEQKFSGMKLVEPGFSTTGADKSKSGGFAHTAIEVGSSLTAGIGGTVAFSLARTAP
                     LPPMAKALTLPVAMLAGGVLKYGSKTALEHAALAPEERTASSADLAWGAVDAVSGIGA
                     STVEKMVATRYFTSLGRAELGATVAAPVAQHAGYMLAKESLSNGIKSNMVRGITGGAT
                     GAAIWSLPHRTSENWQEIKQDPYQGLSKTATQLVGDAGLGAFMGGGLGFAGTTIVRYK
                     DVAGKLKAAVMPEKNLLRLDNYHINDFHSNTEIMPRLTTLVGKLQQDSARRGVDARFI
                     VPGDVESGRVNFAFTKGGQIENEVLMRAGAKEFVPGNHAYDAPGGKGDVARYPAVMEP
                     LLQKHPDVSLLAANLDVSAYPQYQRILKPYTVRTVVQDGVEHKVGT"
BASE COUNT          477 a          605 c          538 g          348 t
ORIGIN      
        1 ccttaagacc ggtataacga cgccattcct tcaagaaaca atcccgtccc cgcacatagg
       61 cggcaagcct ggtgagaagt tcctcggcac cggtatcgtt gtcggccgcc aggcaggaca
      121 ggcatagtaa gtgtttctcc tcgcccagag ccagattcaa gacctgctgc cgcaaacaca
      181 actgcttctg acagagcgca caacgctccg ccattccatc catcaattga actgcacaac
      241 cggtcaaagg ccacatcccc aagtgcttct atgcaaacca agtttaaccc ctcccgcccc
      301 gaggcaagac actggcttcc agccaggaac ggaaagagcg ggaaaagata agacgcgctt
      361 aagctggtcg actttagtgg gaaggctacg cagctcccgg gatcttccac ccttaccaaa
      421 atgaatatat agagttaatc ttgcagcaag cgttgccaag aggacacaag aggacataag
      481 gttggccttc acccccgaag tagcaatccg gacatcacaa actcccactt ctgacgcgct
      541 cgatagagcg cttttagacc catctaaagt cggcttcaag gcaggaaaga cttcagatca
      601 aggtcgagag ccgcagaaga ccgagaatga gagcgcctcc gtgcgcctca ttcaagagtt
      661 gcaagagccg ggcaaagcgc aaaagagcag cgtcgccact aatgtggaag gcaagactgc
      721 ggcagcatcc gagcccgggc cgtccactga aaccaaagac cagttgcgcc tctcaggact
      781 gggctccctc accgccgaac agaaattcag cggcatgaaa ttggtagaac ctggtttttc
      841 aaccaccggt gccgacaaat ctaagtccgg cggtttcgct catacggcca tcgaagtggg
      901 gtccagcctc acggccggta tcggcggcac ggtagccttc tccctggccc gcacagcgcc
      961 cctgccgccc atggccaaag ccctgacact accggtggcc atgctggccg gcggtgtcct
     1021 gaaatacggc tccaaaacag ctctcgaaca tgccgccctg gccccggaag aacgcaccgc
     1081 ctccagtgcc gacctggcct ggggagccgt ggatgccgtt tccggcatcg gtgcttccac
     1141 cgtggaaaag atggtagcca ctcgttattt caccagcctg ggacgggcag agttaggggc
     1201 cacggtagcg gcgccggtag cccaacatgc cggctacatg ctggccaaag aatctctttc
     1261 aaacggtatc aaatcgaaca tggtgcgcgg catcaccggt ggtgccacgg gcgccgccat
     1321 ctggagtctg ccccaccgga ccagcgaaaa ctggcaggag atcaagcaag atccttatca
     1381 ggggctgtcc aagaccgcca ctcaactggt gggcgacgcc gggctgggag ccttcatggg
     1441 cggtggctta ggctttgccg gcaccaccat cgtgcgctac aaagatgtgg ccggcaagct
     1501 gaaagcagcg gtcatgccgg agaaaaatct gttgcgcctg gacaactatc acatcaacga
     1561 cttccactcc aacactgaaa tcatgccgcg actgacgacc ctggtgggca aattgcagca
     1621 ggattcggca agacgaggtg ttgatgcccg tttcatcgta ccgggcgatg tcgagtcggg
     1681 gcgggtgaat ttcgccttca ccaaaggcgg ccagattgaa aatgaagtat tgatgcgggc
     1741 cggcgccaag gaatttgtac cgggtaacca cgcctacgac gcccccggcg gcaaaggcga
     1801 cgtggctcgt tatccggccg tgatggagcc ccttctgcaa aaacatcccg atgtatcact
     1861 tcttgccgcc aacctggatg tcagtgcata ccctcagtac cagcgcatcc tcaaacccta
     1921 caccgtgcgt accgttgtgc aggacggcgt cgagcacaaa gtgggcac
//
LOCUS       DAOQNZ010000828         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39270_length_1966_cov_4.039770, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000828 DAOQNZ010000000
VERSION     DAOQNZ010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39270_length_1966_cov_4.039770"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKW73_13820"
     CDS             <1..384
                     /locus_tag="PKW73_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA74418.1"
                     /translation="PAVDGPEGGFKIASPSFNEVVTPATVNRTASGAEAEALFQEQVA
                     RCFPSVQNKCLRSAVCMYTDTHDSRFVIGRLPGLSSVLIASPCSGHGFKHSPAIGECL
                     AELATTGTSSIDLSGFAIEKAIAAV"
     gene            complement(430..>1966)
                     /locus_tag="PKW73_13825"
     CDS             complement(430..>1966)
                     /locus_tag="PKW73_13825"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNA74419.1"
                     /translation="KPDASGAAAAEGKPLEIVDKTAEYADKFKEAAEVEARAKEKADA
                     PPKITVSLKDNSGEPAERKEPHYVVKRDGKVEMHGDPVKLGAKDVRVEVEREPGEVNP
                     SAEQQAATDKLVAQVNADLQKAFPDQADKVVLDDQGDVVSRKTEQATGLKPPAEQSKL
                     SPETRESVDQTKRLHRSGGVDMPRAATEGMGSFETRDVPRQKGETDKVAAGKEAWAGL
                     FKPDKDHPYETVRKTPEGEHRVGRYGFNGNQLESWLSSLTPEMIDKLIAEGKLPKSFA
                     NPEFLAKFKEFAAKLKNGEQPTPAEMKQFLPKEAQETIAGKLLDDMNKATGDNAGMSA
                     AGLVSGKTAQTMDQTFMTSPEAQELSRAGKTLFDVATQRQLIEGDGSKLGRLPEGDRR
                     ELVEKALQIAGQPVTEANILAVNTIVQHESSWNPGITNNWDINAQRGIPSQGLMQTIP
                     PTFNAHKDRSGQVEAAGHSNTIKDPLANLVAGIKYSEHRYGSLQNVPGVRSMAQGGGY
                     KPY"
BASE COUNT          369 a          581 c          534 g          482 t
ORIGIN      
        1 cccgcagtcg acggaccaga gggcggcttc aaaatcgcca gccccagctt caacgaagta
       61 gtcactcccg ccaccgtcaa tcgcaccgcc agcggtgcgg aagccgaggc tctctttcaa
      121 gaacaagttg ctcgctgctt tccctcggtg caaaacaaat gcctgcgctc ggccgtttgc
      181 atgtataccg acacccacga ctcccgtttt gttatcgggc gactgccggg tttgagctct
      241 gtgctcattg cttcgccctg ctccggtcat ggcttcaaac attccccggc cataggcgaa
      301 tgtctggctg aattagccac aacaggaaca agcagcattg atctttccgg ctttgccatt
      361 gaaaaggcca tagcggcagt ataagtcaga gtcgactaaa tttgagccga ctatatccga
      421 ccacagaacc taataaggtt tgtagccgcc accctgggcc atcgaacgga caccgggtac
      481 attctgcaaa gagccatagc gatgctcgga atacttaatg ccggccacca ggttggccag
      541 tggatctttg attgtgttgc tgtgtccggc tgcttccacc tgtccgcttc tatctttatg
      601 agcattgaaa gtaggcggaa tggtctgcat caagccctgg gaaggaatgc cgcgctgggc
      661 gttgatatcc caattatttg taatgcctgg attccaacta ctctcatgct gcacgatggt
      721 gttgacggca agaatattgg cttccgttac cggctgtccg gcaatttgca aagctttctc
      781 gaccaactcg cggcggtcgc cttccggcag gcgacccaac ttactgccat cgccttcaat
      841 gagctggcgc tgggtggcaa catcgaagag ggtcttaccg gctcgggata gctcctgggc
      901 ttcgggcgag gtcatgaaag tttgatccat ggtctgggcc gtcttaccgg aaaccagtcc
      961 ggcggcggac ataccggcat tgtcgccggt ggccttattc atatcatcga gcaacttacc
     1021 ggcgatggtt tcttgcgctt ccttgggcaa gaactgcttc atctcggcag gtgtgggctg
     1081 ttcaccgttc ttgagcttgg cggcaaattc cttgaacttg gccaggaatt cgggattggc
     1141 gaaagacttg ggcaatttgc cctcggcaat gagcttatca atcatctcag gggtgagaga
     1201 actgagccag gactcaagct ggttgccgtt gaagccataa cgaccgacac ggtgttctcc
     1261 ttccggagtc ttgcgcacgg tttcataagg atggtctttg tcgggcttga acaagccggc
     1321 ccaggcctct ttaccggcgg cgactttgtc tgtctcaccc ttctgcctgg gcacgtccct
     1381 ggtttcaaag gaacccatgc cttcagtggc ggcccttggc atatccacac caccggagcg
     1441 gtgcagacgc ttggtctggt cgacggattc tctagtctcg gggctcaatt tagactgctc
     1501 ggccggcggc ttcaagcctg tggcttgctc ggttttgcgg gagacaacgt cgccctggtc
     1561 atccagtacg accttatcgg cctgatcggg gaaagccttt tgcaagtcgg cgttgacctg
     1621 ggccaccagc ttgtcggtgg cagcttgctg ctcagcgctg ggattgacct caccgggctc
     1681 gcgctctact tctacccgca catccttggc acccagcttg accggatcgc cgtgcatttc
     1741 cactttaccg tcacgcttca ccacatagtg aggctctttg cgctcggcag gctcgccgct
     1801 attgtccttg agcgagactg taatcttggg cggagcatcg gctttctctt tcgccctggc
     1861 ctcaacctcg gccgcttcct taaacttatc ggcatactca gccgtcttat cgacaatttc
     1921 caggggcttg ccctcggcag cggctgcgcc gctcgcgtcc ggcttg
//
LOCUS       DAOQNZ010000829         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39271_length_1966_cov_4.013082, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000829 DAOQNZ010000000
VERSION     DAOQNZ010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39271_length_1966_cov_4.013082"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKW73_13830"
     CDS             <1..315
                     /locus_tag="PKW73_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74420.1"
                     /translation="IVVATQPGRQAGQVLHTCKYSLERGYLDPPDAVASLTADLLLGR
                     VEIEQYLLQRRRMTGDELRDLVDLARQKGVKLVELLVKIGFMTQSDWERLSAEKDRFL
                     PR"
     gene            572..1591
                     /gene="trpS"
                     /locus_tag="PKW73_13835"
     CDS             572..1591
                     /gene="trpS"
                     /locus_tag="PKW73_13835"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNA74421.1"
                     /translation="MSAKRIMSGMRPTGRLHLGHYFGALRNWIHFQSQYESFFSIVDW
                     HALTTKFQNTREVQPHIWEIALDWLCVGIDADVATIYVQSAVPEIAELHLLLSMFTPH
                     NWVEREPTLKDMVKMLDQDESAAKEKVTYGLLGYPVLMASDILTFRGELVPVGKDQES
                     HLEFARDVARRFNNFYNVDYFPEPKPEFTTTPLLKGIDGQKMGKSFNNDIKIADTDDD
                     TIAKVKKMITDRTRIAKSDPGHTDLCEVPWPMYQIFAGDPSLLASVKSDCESAAIGCV
                     DCKVKLAGLINDYFRPMREKRRELAANPGKVREIVEEGNLKARKVARQTLKDVREIMG
                     MVNWA"
     gene            1621..>1966
                     /locus_tag="PKW73_13840"
     CDS             1621..>1966
                     /locus_tag="PKW73_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74422.1"
                     /translation="MLSRLSLFLTALLALSLSVSLTLSWVVLLALMAVKFIEYGPRAC
                     WQSVQGAPFFYPLLAFISVCLLSGLWNGGPVEGLASFSTTRALLIYAVGYLVFACEKD
                     RKTALAVFLTVAM"
BASE COUNT          459 a          490 c          542 g          475 t
ORIGIN      
        1 atagttgtgg ccacccaacc gggtcgtcag gccggacagg tgcttcacac ctgtaagtac
       61 tccctggaaa gaggttacct ggatcctccc gatgccgtag ccagtttgac cgccgatctg
      121 ctcctggggc gtgtggagat tgagcaatac ctgttgcaaa gaagaagaat gaccggcgat
      181 gaactgagag acctggtgga tctggcccgg cagaaaggtg tcaagctcgt ggagcttcta
      241 gttaagatag gatttatgac tcagtcggat tgggagcgat tgtccgccga gaaggaccgg
      301 tttctgcctc ggtaaggcga ggggaccgtt atccggcgca agtgttcagg cccgagagac
      361 tttacaagtc ttttttttag ccggggaagt tctctttcgg ctcagacctt tgggtaaact
      421 aaggcttccc tctatatcta aaggagtcag ttgtgacaac taagtacaac gcccgtatca
      481 agaaaaagcg ccgccttgct aaaaacgaca gaaagaaaga gaaagtaaga gaagcaatcg
      541 ccaaggctgc caagtaactc agggataaca aatgtctgca aagcgaatca tgtcgggcat
      601 gcgcccgacc ggacggttac accttggcca ctattttggg gcactgagga actggattca
      661 tttccagagt cagtatgagt cgttcttttc catagtggac tggcacgctc tcaccaccaa
      721 gtttcagaac accagggaag tgcagcccca tatctgggag attgcactgg attggctttg
      781 cgtcggtatc gatgcggacg tggcgacaat ttatgttcag tcggcggtac cggaaattgc
      841 agaattacac cttctgcttt ccatgttcac tcctcacaat tgggtggagc gcgagcccac
      901 actcaaggac atggttaaga tgctcgacca ggatgagtcg gcggcaaagg aaaaggtcac
      961 ttacggtttg ctgggttatc cggttctgat ggcttccgac atcctcacct tccggggcga
     1021 acttgtaccg gtgggcaaag atcaagaatc ccatctggag ttcgccagag atgtggctcg
     1081 gcgcttcaat aatttctata acgtggatta ctttccggag cccaagccgg aatttaccac
     1141 aacccccctt ctgaagggca tcgacggtca gaaaatgggc aagtcgttca acaacgatat
     1201 taagattgct gataccgatg acgataccat cgccaaagtc aagaaaatga tcaccgaccg
     1261 cactcgtatt gccaagtcgg atccagggca taccgatctt tgcgaagtac cctggcccat
     1321 gtatcagatc tttgccggcg atccgtccct gttggcgtcc gtcaaaagtg attgtgaatc
     1381 ggcagccata ggctgtgtcg attgcaaggt aaagttggcc ggtctcatca acgactattt
     1441 ccgaccgatg cgggagaagc gcagagagct ggctgctaat cccggtaagg tgcgagaaat
     1501 agttgaagaa ggaaacctga aggcacgcaa ggtggccaga caaaccttga aggatgttcg
     1561 ggaaatcatg ggtatggtca actgggctta gggtcttgcc ctcagaaaaa tggttcaggc
     1621 atgctcagcc gactttcact tttccttacg gctctcttag ccctcagtct ctcggtcagc
     1681 ttgacgttgt cctgggtggt gcttctggct ttgatggccg tcaagttcat agagtacgga
     1741 ccccgtgctt gctggcagtc agttcagggg gcgcccttct tttatccctt gctggctttt
     1801 atttccgtct gcctgctttc tggtctctgg aacggcggac cggtggaagg gctggcttct
     1861 ttttcaacca ccagggccct gctcatttat gctgtgggct atctggtctt cgcctgcgag
     1921 aaggaccgaa aaactgccct tgccgtcttc ctgacggtgg ccatgg
//
LOCUS       DAOQNZ010000830         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39389_length_1963_cov_4.085954, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000830 DAOQNZ010000000
VERSION     DAOQNZ010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39389_length_1963_cov_4.085954"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..943)
                     /locus_tag="PKW73_13845"
     CDS             complement(59..943)
                     /locus_tag="PKW73_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNA74423.1"
                     /translation="MNLAISLRRAIELWPDREACVDGMKRLTWRDLGQRVAALTHAFR
                     NLGLTKGSVVACMAPNCVEYLELYYATSCLGLVLAPINFRLSEQEVREILVHSGAEML
                     VCHTDFAEHTTSMLEDLAGAGGETSLKLVTWLGPGHPAKTSFKSMHYEPFILSHWRRE
                     LIDCELYSEDLAQLYYTSGTTGLSKGVMLTHGNVASNALGAIAELGLTDRDIWAHIAP
                     MFHLVDAWAVYAITWVGGKHVFVPYFKADEALKLLETENVTITALVPTMANALLHSGK
                     VWEYSYSKLRAIMTVPEP"
     gene            1226..>1963
                     /locus_tag="PKW73_13850"
     CDS             1226..>1963
                     /locus_tag="PKW73_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNA74424.1"
                     /translation="MGQVIDKTYNGLKVPQGEKITFKDGKPVVPNKPIIPFIEGDGTG
                     RDIWKASKRVIDAAVAKSFGGEKEIAWYEVFAGEKAHDSFKEWLPEDTIAALKEFGVA
                     IKGPLTTPVGGGIRSLNVSLRQIFDLYCCVRPVRYFQGVPSPVVAPEKLNVVIYRENT
                     EDVYAGIEYLAGSPEAKKLIELIESFTGNKKIRPDSGIGIKPISEFGSQRLVRKAIEY
                     AMERGRKSVTMVHKGNIQKYTEGAFRDW"
BASE COUNT          521 a          527 c          520 g          395 t
ORIGIN      
        1 gaagagaaat tcacggagcg agtcgagaaa tcgctgcacc tgtacctgaa cgtcactatc
       61 acggctctgg taccgtcatg atcgctctca gcttcgaata ggaatattcc cagaccttgc
      121 cggagtgcaa gagagcatta gccatagtcg gtaccagagc cgtgatagtg acgttctccg
      181 tttccagaag cttaagggcc tcgtcggctt taaagtaagg cacaaatacg tgcttgccgc
      241 ccacccaagt gatggcataa acagcccagg catcaaccag atgaaacatc ggggcgatgt
      301 gggcccagat gtcgcggtcg gttagtccca gctcggcgat ggcccccagg gcattgctgg
      361 ccacattgcc atgggtaagc atgacgccct tcgataggcc ggtcgtgccg ctggtgtaat
      421 agagctgagc gagatcttca ctgtagagtt cacagtcaat aagttcccgg cgccagtggc
      481 tgagaatgaa aggttcatag tgcatagact tgaaggaagt cttagccgga tgaccgggtc
      541 ccaaccaggt caccagttta agactggtct cgccgccggc cccggccagg tcttccaaca
      601 tgcttgtggt gtgctcggca aaatcagtat gacagaccag catttcagcg ccgctgtgca
      661 caaggatttc ccgtacctct tgttccgaaa gacgaaagtt tatgggagca agaaccagac
      721 caaggcagct tgtagcatag taaagctcca ggtactcgac gcagttggga gccatgcaag
      781 ccaccacaga gcccttggtc aagcccagat tgcgaaaggc atgagtgaga gccgccaccc
      841 gttgaccgag gtcgcgccag gtaagccgct tcattccatc cacacaagct tcgcggtcag
      901 gccagagctc gatggctcgg cgaagggaaa tggccagatt caaaccttat tacctcgaag
      961 cggaacagaa caagatgaaa gtatatacaa ccgccaggca cggcgcctcc accgagcgtt
     1021 aatatgagtt catgcaagct cctcttgata tccagcgacg ggagccgata ttacttttta
     1081 attactgttg gaagggtagc cgcccactgg ctgttaacct ttttagatat ctggtcgaac
     1141 cggcaataca caggtgagac cataaagccg ccatgatcat ggcacaagca caagcactca
     1201 ggttaggaat aaggtataag agacaatggg acaggttatt gacaagacat ataacggctt
     1261 aaaagttccc cagggtgaga aaatcacttt caaagacggg aagccggttg taccgaacaa
     1321 gccgatcatc cccttcatcg aaggcgacgg caccggtcgt gacatttgga aagccagcaa
     1381 gcgcgttata gacgctgctg ttgccaaatc cttcggcggc gaaaaggaaa tcgcctggta
     1441 tgaagtgttt gccggcgaaa aagcccatga cagcttcaaa gagtggctgc ctgaagatac
     1501 catcgctgcc ctcaaagaat tcggcgtggc catcaaagga cccctgacca ccccagtggg
     1561 cggcggcatc cgcagcttga acgtcagctt gagacagatc ttcgatcttt actgctgcgt
     1621 gcgcccggtc cgttacttcc agggcgtgcc ttcaccggtt gttgcacccg agaaactgaa
     1681 cgtagtcatc taccgcgaga ataccgaaga cgtttatgcc ggtattgaat acctggccgg
     1741 ttctcctgaa gccaagaaac tgattgaact gatcgaatct ttcaccggca acaagaaaat
     1801 ccgccccgac tccggcatcg gcatcaagcc catctccgag ttcggctccc agagactggt
     1861 cagaaaagcg atcgaatacg ccatggaaag aggacgcaag tctgtcacca tggtacacaa
     1921 aggcaatatt cagaagtaca ccgaaggcgc cttcagagac tgg
//
LOCUS       DAOQNZ010000831         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39393_length_1963_cov_3.856918, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000831 DAOQNZ010000000
VERSION     DAOQNZ010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39393_length_1963_cov_3.856918"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKW73_13855"
     CDS             complement(<1..189)
                     /locus_tag="PKW73_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74425.1"
                     /translation="MQNLELMLIEALRRDALRQQEGRLSEVGKEFDEAEEYRSLHRGE
                     SSSKELGIALTFWDAWVDQ"
     gene            complement(258..725)
                     /locus_tag="PKW73_13860"
     CDS             complement(258..725)
                     /locus_tag="PKW73_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74426.1"
                     /translation="MTSDQEADRKPAEAVSEQSPEVQEKPSETAPMEESSVSLKPKAG
                     PFKTVVFVLTMAGVALTLAGIVYDAQSQLETTFKSPDGKFAVEVYAYPRLLPGKAPKE
                     KGPGKVRLVTGSGVPLDQAPLNTLSSMSEPRWTKDRVHIENVADFVLPEEQGK"
     gene            complement(813..1253)
                     /locus_tag="PKW73_13865"
     CDS             complement(813..1253)
                     /locus_tag="PKW73_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74427.1"
                     /translation="MKNFFRMGLGFETSLVFLPLAEVKGGLTMGLLMADTGGDQMADA
                     RHSRVSDERIRELTESIENGNMTALTKFLNRLSSSSDRLDVLERIEKLNNENRYRSGR
                     TPRLALEQRTYPDSDFRDIALLKKSTDWLFQDDVIYKESIVYQH"
     gene            complement(1269..>1963)
                     /locus_tag="PKW73_13870"
     CDS             complement(1269..>1963)
                     /locus_tag="PKW73_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74428.1"
                     /translation="IVSGIVLALIVAAGSCIALFVRQEMKIDGLLRAEKFLEAAVCVQ
                     TAALPVVLGPHRDGGAADLYLLAYLSERAGDKKLAAESLLRADAELPTSAVVKATENL
                     ESAASMLQYRLHLGYLSEACLRAGVQLARRRQEAGPTGRRLLAKSLLALGRDYRLNGK
                     FNLAARAQAEAISVCEQALGEREAAREVDREMEALLKDCLSAQLELFRRFGQLDDCKP
                     LLQRLERLESAK"
BASE COUNT          462 a          588 c          446 g          467 t
ORIGIN      
        1 ctgatccacc caggcatccc agaaagtcag ggcgatacca agttctttgg agctcgactc
       61 tcctcggtgg aggctgcggt actcctcagc ctcatcaaac tccttaccga cctctgaaag
      121 ccgcccctct tgctgcctga gagcatctct gcgcaaagcc tcaatcaaca tgagctccaa
      181 gttctgcatt tttgaatact tctcggctgc caaacaaaac tctcgtaccg taacggactc
      241 ttcccgctct tgctggacta ttttccttgc tcctcaggaa gaacgaaatc ggcaacgttt
      301 tctatgtgca cacgatcttt tgtccatctg ggctcagaca tcgatgaaag cgtattgagc
      361 ggcgcctgat cgaggggcac accactgccg gtgacaagcc gcactttgcc gggacctttc
      421 tctttcggtg ctttgccagg caaaagccgt ggataggcat aaacctccac ggcaaacttg
      481 ccatccggac ttttgaaggt agtctcaagc tgcgactggg catcgtatac gataccagcc
      541 agagtcaaag ccacaccggc catggtcaat acgaagacca cggttttgaa gggtccggcc
      601 ttgggcttaa gcgatacact cgattcttcc atgggggcag tctctgacgg tttctcttga
      661 acctcgggag actgttctga cactgcctca gcgggcttgc ggtcagcttc ttgatcactt
      721 gtcacggcat ttcatcctcc agagtaatga tactccttcg gaaagccttt atggattgct
      781 cggggttgaa ttgtcaaaat tcaactacaa catcaatgtt gataaacaat cgactctttg
      841 tagatcacat catcttggaa gagccaatcg gtgctctttt tgagcagcgc gatgtctcta
      901 aaatcactat cggggtaggt tctctgctca agggcaagcc ggggagtccg accgctcctg
      961 tagcggtttt cattattgag tttctcaatt ctctcaagca cgtccaagcg atccgaagaa
     1021 gagctcagtc gattgagaaa cttagttaac gctgtcatgt tgccattttc aatagattcc
     1081 gtaagctcac gaattctttc gtcacttaca cgagagtgtc ttgcatctgc catttggtca
     1141 ccccctgtat ccgccatcaa caaacccata gtaagcccac ccttgacttc cgcaagtggt
     1201 aaaaatacta atgaagtctc gaacccaagg cccatgcgga aaaagttttt caagcaataa
     1261 aattccgctc actttgcaga ctcaagtctt tcaagacgct gcaaaagcgg cttgcaatca
     1321 tcgagctgac caaagcgccg aaacaactcc agttgcgcgc tcaagcagtc tttcagcagt
     1381 gcttccattt cgcgatcaac ttccctggct gcttcccgct ctcctagagc ctgctcgcac
     1441 accgagatag cttccgcctg agcacgggct gccagattga atttaccatt gaggcgatag
     1501 tcgcgcccca gggccagcag tgacttggcc agcaatctcc ggccagtcgg gccggcttcc
     1561 tgacggcgcc ttgctagttg cacaccggct ctcaaacagg cttccgacag ataacccaaa
     1621 tgcaatctat actgcagcat cgaggcggca ctttccaggt tctcggtggc ctttactacc
     1681 gcggacgtcg gcagttcggc atcggcccgc aacaggctct ccgccgccag cttcttgtcg
     1741 ccggctctct cgctcaaata tgcaagcaaa taaaggtcgg ccgcaccgcc gtcccgatgc
     1801 ggccccagca ccaccggtag tgcggcagtc tgcacacaga ctgccgcctc aagaaatttc
     1861 tccgcccgca acaaaccatc gattttcatc tcttgccgga caaacaacgc tatacacgag
     1921 ccggcagcca caattaaagc cagcacaatg ccagagacga tct
//
LOCUS       DAOQNZ010000832         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39429_length_1962_cov_3.663346, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000832 DAOQNZ010000000
VERSION     DAOQNZ010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39429_length_1962_cov_3.663346"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..793
                     /locus_tag="PKW73_13875"
     CDS             245..793
                     /locus_tag="PKW73_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNA74429.1"
                     /translation="MIADMSHAAHRSASSAGNDHGDRELKTPAVFDGLGGFRDLLDRR
                     DVAPQGKDNNLEWNTDIRQAMKSAREQGLPLVMHFTLAKEGSCDWCTAFDKQLADPSL
                     KSLQDKAVFLRIPVDPDKVTDGSRLAEIMKIQGYPTISVVNIGNAGVNELRRMSGYQT
                     SGNLKAKLEDTFRPGAEAMTLV"
     gene            886..>1962
                     /locus_tag="PKW73_13880"
     CDS             886..>1962
                     /locus_tag="PKW73_13880"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNA74430.1"
                     /translation="MQTEQTEVQDVLTFADKTRSDYEKTLKAFVDLASVSMEPERRPE
                     IDATAEMGAKLLKSMGAAAEVVKTAGYPIVMGEFHRGSQYPTVTVYNHLDVQPALASE
                     WNSPPFDMQIENGVYRGRGTTDDKGPALAVLNAAHYAHNQGLPINIKFIWELEEEIGS
                     PSFDQFLKDNLGKLSTSSVVVSDTIWVSRERPAIPYGLRGLQAVLIRLKTGVKDVHSG
                     TTGGLARNPIGEMAQLLNSIYDAKTGEVKIPGFYDKVKPVDQDELDNFVASGFTVANF
                     KCAHELTHTRTEDARDGARRIWAHPTFEVHGITGGYAGPGVKTIVPYQCEAKVSMRLV
                     PDQDPEATFNLLKEFVEKQGDFQVL"
BASE COUNT          468 a          536 c          530 g          428 t
ORIGIN      
        1 cccagatgat cgcccggggc gatatcaata aacggggtgt gctcaggcag gagcaggccg
       61 tacccactcg cctcttcctc accgaaatgg acggtcgggg catcaatctg aagatgatcg
      121 agcgcagccc ggccctgaaa aactaatttt cggatactcc gatttaatgt ccacaatttc
      181 ttgtccggcg tatagaaacc ggcacaagcg ttgcgggata ctaattagta ctaggagttc
      241 atccatgatt gccgatatgt ctcatgccgc ccatcgatca gcctcctctg ccggcaatga
      301 tcatggcgac agggagctga aaacgccggc cgtctttgat ggtttgggcg gatttagaga
      361 cctgcttgac cggcgggatg tggcgccaca aggcaaggac aacaatctgg aatggaacac
      421 cgacattcgc caggccatga aaagtgccag agagcagggt cttcccctgg ttatgcattt
      481 caccctggct aaggaaggtt cttgcgattg gtgcacggct ttcgataaac agctggccga
      541 tcccagtctc aaaagcttgc aagataaggc tgttttcctg cgcatccccg tggatccgga
      601 taaagtcaca gatggcagcc gcctggcgga gataatgaaa attcagggct atccgactat
      661 ttccgtagtc aatataggca atgccggcgt aaatgaattg cgccgcatga gcggctatca
      721 gacgagcggc aatctgaagg caaaactgga agatactttc agaccgggcg cggaagccat
      781 gaccctggtt taagaatttc ccgccttcgc cgttgccagg gctctggcca agcggtgata
      841 attgctccct gtaataagag aggctgcttt cagcgggggc gatcaatgca aactgagcaa
      901 actgaagttc aagatgtcct gacctttgcc gacaaaacca gaagtgatta cgaaaagact
      961 ctcaaggctt tcgtagatct ggcttccgtc agcatggagc cggagcgccg ccccgaaatt
     1021 gatgccacgg ctgagatggg tgccaaactg ctcaaatcca tgggtgctgc cgccgaggtg
     1081 gtgaagacgg ccggctatcc gattgtgatg ggagaatttc accgcggcag tcagtacccc
     1141 accgtcaccg tctacaacca tcttgatgtg caaccggccc tggccagtga atggaactcg
     1201 ccgccctttg atatgcaaat tgaaaacggc gtctacagag ggcgtggcac caccgacgat
     1261 aagggcccgg ccctggccgt cttgaatgcc gcccactatg cccataatca ggggctgccc
     1321 atcaatatca aatttatctg ggaactggaa gaggaaatcg gttcaccttc cttcgatcag
     1381 tttctcaaag acaatctggg caaactctcc actagttccg tggtggtctc cgataccatc
     1441 tgggtctccc gcgaaagacc ggctattcct tacggactgc gtggtcttca ggccgtcttg
     1501 attcgcctca agaccggtgt caaagacgtt cattccggca ccaccggcgg tctggcccgc
     1561 aaccccatcg gcgagatggc ccagcttttg aacagcattt acgatgctaa aaccggcgag
     1621 gtgaaaattc ccggttttta cgacaaagtt aaacctgtgg accaggacga actggataac
     1681 ttcgtggcct ccggcttcac cgtggctaat ttcaagtgcg cccatgaact gacccatacc
     1741 cgcacggaag atgcccggga tggtgcccgc cgcatctggg ctcatcctac tttcgaggtg
     1801 catggtatca ccggcggtta tgccggtccg ggcgtcaaaa ccatcgtgcc ctatcaatgt
     1861 gaggccaaag tcagcatgcg tctggtgcct gatcaggacc cggaagctac cttcaatttg
     1921 ctcaaagaat tcgtggagaa gcagggtgat ttccaggttt tg
//
LOCUS       DAOQNZ010000833         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39442_length_1962_cov_2.861038, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000833 DAOQNZ010000000
VERSION     DAOQNZ010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39442_length_1962_cov_2.861038"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKW73_13885"
     CDS             complement(<1..328)
                     /locus_tag="PKW73_13885"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNA74431.1"
                     /translation="MTEATWLSVLSLMHLVGMVKHYSEQLDRTFFALSDPTRRAILNN
                     LALKGSCSVLELAEPFSMSLPAISKHLKLLESARLVERVRKGRVHYLKLAAAPMEEAN
                     DWLLAYR"
     gene            complement(512..724)
                     /locus_tag="PKW73_13890"
     CDS             complement(512..724)
                     /locus_tag="PKW73_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74432.1"
                     /translation="MNITETTTVKEILDAYPLAVKVFEEHGVDVPLECDESILDTELS
                     ICDGMCHIDDLSELISDLKKFIAEAG"
     gene            complement(760..1548)
                     /gene="ric"
                     /locus_tag="PKW73_13895"
     CDS             complement(760..1548)
                     /gene="ric"
                     /locus_tag="PKW73_13895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNA74433.1"
                     /translation="MCCEKQEPCTPRHFATASRRESEPLDEKSIGLMVAEEPRRAAVF
                     ESFAIDYCCGGKRTLAQACENAGVSIELVKSAIGELPALPGDTEPNWLQADLKELACH
                     IESTHHAFLKKELPRLKLLSEKVAAVHGSKDERLIKVREVFAALKEELEEHTLKEERV
                     LFPYICALASARAPIPVPFGTVANPVRCMEAEHDEAGNALSRLRELTDSFQAPASACA
                     SWTALLAGFAALEQDLHRHIHKENSILFPRAVVAEEALMRNAVH"
     gene            complement(1569..1778)
                     /locus_tag="PKW73_13900"
     CDS             complement(1569..1778)
                     /locus_tag="PKW73_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNA74434.1"
                     /translation="MKYICTLCNYVVEDSRKPIANLPEPQVPFENLPDDWHCPECAAV
                     KEFFQPCSCASLHLYEATKVAVDAA"
BASE COUNT          514 a          537 c          457 g          454 t
ORIGIN      
        1 ggcggtaagc cagaagccag tcattggctt cctccatagg tgcggcggca agcttgaggt
       61 agtgcacccg acctttacgc acccgctcga ccagtctggc gctctctaaa agtttgaggt
      121 gctttgatat agccggcaat gacattgaaa acggctccgc caactccagt acggaacaag
      181 atcccttcag ggcaagatta ttgaggattg ccctgcgggt gggatcggaa agcgcgaaga
      241 aggttcggtc taattgctcg gaataatgct taaccatacc caccaaatgc atgagactca
      301 aaaccgaaag ccatgtggct tcggtcaaaa gaccaatttc aacgatggcg tcattaaacc
      361 atatcgttaa ataagatgca agagcccgat tatttgaccg gcaccagagc ggatatcgtc
      421 tcgctgccgc actgagccgg gaaatctgcc gggagccact ggtttagatc atctatctca
      481 ccggcaaagt tagtcggagc tcgacggctg gctacccggc ttcggctatg aactttttca
      541 agtcggaaat cagctcgctg agatcatcaa tgtggcacat gccatcacaa atggaaagct
      601 cggtatcgag gatgctctca tcacactcca ggggaacatc gactccatgc tcctcaaaga
      661 ccttcacggc caacggatat gcatccaaaa tttctttaac agtggttgtc tcagtaatat
      721 tcatggcaca cctcacttta cttaattttc aaaaccaacc taatgcaccg cattccgcat
      781 cagggcttcc tccgccacca cagcccgagg gaagagaatg ctgttctctt tatggatgtg
      841 gcgatgcagg tcctgctcta aggcggcaaa accagctagt agagcagtcc aactcgcaca
      901 agccgaggcg ggtgcttgaa aggaatccgt caactcccgt aaccgagaga gagcattccc
      961 agcttcatca tgctctgctt ccatacaacg tacagggtta gctacggtgc cgaagggcac
     1021 cggtatggga gcgcgagccg aagcaagcgc gcaaatatat ggaaacagca cgcgctcctc
     1081 cttcaaggta tgctcctcca attcctcttt caaggcagca aaaacttctc gcaccttaat
     1141 gaggcgctca tctttgctgc catgtaccgc ggccaccttc tcagagagca acttgagacg
     1201 cggcaattcc tttttgagga aggcatggtg agtgctttca atgtggcaag ccagttcctt
     1261 tagatcagcc tgaagccaat ttggttcagt atcgccaggc aaggcgggaa gctctccaat
     1321 agcgcttttt acaagctcta ttgacacgcc ggcattttca caagcctggg ccagcgtgcg
     1381 cttaccacca cagcaatagt caatggcaaa actctcaaac acggcagcac gccttggttc
     1441 ttccgccacc atgagaccga tggacttttc gtcgagaggt tcagattctc tgcggctggc
     1501 agtcgcaaaa tggcgcgggg tgcagggctc ttgcttttcg caacacataa ttaccgacct
     1561 cccctcattc aagcggcgtc aaccgctact tttgttgctt catacaaatg cagactggca
     1621 cagctacagg gctggaaaaa ctcctttacg gcagcgcatt ccgggcaatg ccaatcatca
     1681 ggcagattct cgaaaggcac ctgcggctcc ggcagattag cgattggctt gcggctgtct
     1741 tccacaacat aattacaaag ggtgcatatg tatttcattg tcctatcctc cgataatcaa
     1801 cttctggacc tttatgtcca taataacact tcgacagccc aagtcaagcc ccaaaagccc
     1861 cattttatgg tattctgtat ataaaggtag ataaatacac tttatataca gccagaccac
     1921 ggaaactctc aattctttag agtgaatcct cagcaggaaa ac
//
LOCUS       DAOQNZ010000834         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39448_length_1962_cov_2.572627, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000834 DAOQNZ010000000
VERSION     DAOQNZ010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39448_length_1962_cov_2.572627"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PKW73_13905"
     CDS             complement(<1..978)
                     /locus_tag="PKW73_13905"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA74435.1"
                     /translation="MNLANQKQRQPVWFSTALLLLAALTCMPVLKAQAAPVPAPKQSR
                     EAPGKRQKTPEQVPAKAESPANRPIKDKWAIVVGVSKFARPALNLRNADKDARDFYDY
                     LVKEANFAPDHVKLLINEAATRRRVLSELGSKWLPRLAHPDDLVVIFISSHGSASEMD
                     VGGVNYLVVHDTDPDDLFTTGIDMQQLTRLIKSRVHCQRIVIILDACHSGATRASAKG
                     LVRTGNIDAQAVAMGTGQLVIASSEPTQSSWEFKDKPNGVFTACLLSALRTQDKISLD
                     KAFNSTREEVETVVLRERGVLQSPVLKSAWQGNDIILSVLPTDPRPAVTG"
     gene            1117..>1962
                     /locus_tag="PKW73_13910"
     CDS             1117..>1962
                     /locus_tag="PKW73_13910"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF036305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HNA74436.1"
                     /translation="MPTTESLGVRTMPKVEASYPVNKHKKLQDWVSEAAALCQPDRIY
                     WCDGSEEEYNRLCDEMVESGTLIKLNPKKRPGSFLARSAPSDVARVEDRTFVCTTLKE
                     DCGPNNNWMEPETAKIILKDFFKNCMRGRTMYVIPFSMGPLGSPLSQIGVEITDSPYV
                     AVSMKLMTRMGKPVVELLGTDGDFVPCFHSVGHPLSPGEKDVAWPCDAEHKYICHFPE
                     TKEIWSYGSGYGGNALLGKKCLALRIASTMGKEEGWLAEHMLILGVKNPEGKKSYVAA
                     AFPSAC"
BASE COUNT          399 a          489 c          578 g          496 t
ORIGIN      
        1 accggtgact gccggtctgg gatcggtagg taaaaccgaa aggatgatat cgtttccctg
       61 ccaggcgctt ttcaagaccg ggctctggag aacgccgcgc tcccttaaga ctaccgtttc
      121 cacttcttct ctggtgctgt tgaaggcttt gtccaggctt attttgtcct gggtgcggag
      181 ggcgctcaag aggcaggcgg tgaagacacc gtttggtttg tccttgaatt cccaggatga
      241 ttgggtgggt tcgctggagg caattaccag ttgaccggtg cccatggcta ctgcctgggc
      301 gtcgatattg ccggtgcgca caagaccttt ggcgctggct ctggtggcgc cgctgtggca
      361 ggcatcgaga atgatgacga tgcgctggca gtgcaccctt gatttaatca ggcgggtcag
      421 ttgctgcatg tcgatgccgg tggtaaagag gtcgtcggga tcggtgtcgt gcaccaccag
      481 gtagttgact ccgcctacat ccatttcgct ggcgctgccg tggctggaga tgaagataac
      541 caccaggtcg tcgggatgag ccagcctggg cagccatttg ctgcctagtt cggagagtac
      601 tcgcctgcgg gtggcggctt cattaattag cagtttgacg tggtccgggg caaagttggc
      661 ctccttgacc aggtagtcgt aaaagtcccg ggcgtctttg tcggcattgc gcaaattgag
      721 ggcgggacgg gcgaatttgc taacgcccac gactattgcc catttgtctt tgatcggtct
      781 attggcgggg ctttcggctt ttgccggcac ttgttccggc gtcttttgcc gcttaccagg
      841 cgcttcccgg ctctgcttcg gggcaggcac gggggcggcc tgggctttga gcacgggcat
      901 gcaggtgaga gcggctaaga gcagcagggc ggtcgaaaac cagaccggct gcctttgctt
      961 ttggttggcc aagttcatcg cccccattcc atctgtgctg cttctatatt agaaagagaa
     1021 ttagattctg aattcggcct tgcggttgag tcaaggagga catatttcgt tgtattgttg
     1081 taacttccga taacttgtat tcaggagtta tagataatgc cgactacaga gtctctgggt
     1141 gtgcgtacca tgcccaaagt agaagcgagc tatccagtca ataagcataa gaaattgcaa
     1201 gactgggtta gtgaggctgc tgccctttgt caacccgatc gcatttactg gtgtgatggt
     1261 tccgaagagg aatataaccg tctttgcgat gaaatggttg agtccggaac gctgattaaa
     1321 ctcaatccaa aaaagcgccc cggcagtttc ctggctcgtt cggccccttc cgacgtggct
     1381 cgggtggaag acagaacttt cgtctgcacg actctcaaag aagattgcgg ccccaacaat
     1441 aattggatgg agcccgagac ggccaaaata attctcaagg atttcttcaa gaattgcatg
     1501 cgcggtcgca ccatgtatgt cattcctttc agcatgggac cgctgggttc ccccctctcg
     1561 caaataggcg tggaaatcac agactcgcct tatgttgccg tcagcatgaa actgatgact
     1621 cgtatgggca agccggttgt ggaattgctc ggtacagacg gcgattttgt gccttgtttt
     1681 cactccgtcg gtcacccgct ttcgcccggc gaaaaggacg tagcctggcc ctgcgatgct
     1741 gagcataagt acatttgtca cttcccggaa actaaagaaa tttggtctta cggctccgga
     1801 tatggtggca atgctctgct cggtaagaaa tgtctggctc ttcgtatcgc ctctacaatg
     1861 ggcaaggaag aaggctggtt ggccgagcac atgctcattc tgggcgtgaa aaatccggaa
     1921 ggcaagaagt cttatgtggc agcggccttc cccagcgctt gc
//
LOCUS       DAOQNZ010000835         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39627_length_1958_cov_3.117183, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000835 DAOQNZ010000000
VERSION     DAOQNZ010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39627_length_1958_cov_3.117183"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..308
                     /locus_tag="PKW73_13915"
     CDS             117..308
                     /locus_tag="PKW73_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74437.1"
                     /translation="MAHAREPITTIISLVGVALTCFVIAKASWWMLLVAIVVVCFGCC
                     LYKLIDKKDKPGRLVRERV"
     gene            complement(375..>1958)
                     /gene="murJ"
                     /locus_tag="PKW73_13920"
     CDS             complement(375..>1958)
                     /gene="murJ"
                     /locus_tag="PKW73_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNA74438.1"
                     /translation="GKTFSLVALFTVLSKFAGLLRDMVVASAFGTTMIADAYNIATLF
                     TGNILVLFGGLGGPFHSSAVAVLTPRKDSGDCGRLTVQLLVWTGLITGLLALIVFALA
                     PYIVPLVAPGSGSEAEKIKLWHETTMQLRIMSPLILIAGLVGIGCGISNTYNQFFWPS
                     MAPAVASLCIIIACLGFKGSGETGLCLAAGTLAGAIGQFLVQMPGMLKAHPTFRDLKL
                     FSKMEPGMKEYLHMLWPATISTSIGYIYSYVDVFFTSQMGEGGWTALVTANRLVQLPL
                     GVLLTAMLVPILPRFTLQVTEGKIDDLKSELRRALTLLWFLALPMSALLLALPQPIVQ
                     LLFQRGAFNQHSTELVTTVLFFLVPSIFFYVARDLVTRVFYAHKDSKTPYYVGLAAIG
                     VKLFITWFLAVPLAMGLGGIALSTSIITVFNLSLLSILLRKKIGRLGAGKLIKPVGIM
                     ITAACCCGLSAGFGYGYLVQAAAALKTSLKLPLPHLLELAIPLGFAAGIGGLVYMAIC
                     YLFKLDEVKQITKRLARRG"
BASE COUNT          473 a          474 c          554 g          457 t
ORIGIN      
        1 agggatgaag atctctgccg ttttcttcga tcgtatcgac gattgatatt gtttgcatgt
       61 tgtattgtat atccacttct gaaaattgaa taactgtctc cttgggagga tcggatatgg
      121 ctcatgcccg agagccgatt acgacgatca tctcattggt tggcgtggcg ttgacttgct
      181 ttgtcatcgc caaggccagc tggtggatgt tgcttgtggc aattgtcgtc gtatgcttcg
      241 gttgttgtct ctataaactc atcgataaga aagataagcc cggccgtctt gtgcgtgagc
      301 gagtttaatt tgctgcggca ttttgtaagc tttgttctgt ttctaaggtt cgtcccaggt
      361 gcctgaccgg ttctctagcc ccggcgcgcc agcctcttgg taatttgttt tacctcatca
      421 agtttgaaga ggtagcaaat tgccatataa accagaccgc cgatgccggc cgcaaaacca
      481 aggggtattg ccagctccag aaggtggggc aggggtagct tcagtgaggt tttcagggcg
      541 gcggcggcct ggaccagata gccatatccg aagccggcac tgagaccgca acagcaggcg
      601 gctgtgatca tgatgcccac cggcttgatc aatttgccgg cgcccagcct gccgattttc
      661 ttgcgtaaga gaattgataa caggcttaaa ttgaagacag taataattga tgtggaaagg
      721 gcaataccac ccagacccat ggccagcgga actgctaaga accaggtgat aaagagtttg
      781 acgcctattg cggccagtcc tacgtaatat ggcgttttgg aatctttgtg ggcatagaaa
      841 actcgcgtca ccagatctct tgccacgtag aagaatatgc tcggcaccag gaagaacagt
      901 accgtcgtta ccagctctgt ggaatgttga ttgaaagcgc ctctctggaa caggagctgc
      961 acgattggct gcggtagcgc taagagcaag gccgacatgg gtagggccag gaaccagagc
     1021 agggtaaggg cgcgccgcag ttcgctcttt aaatcatcaa tcttgccttc ggtcacttgc
     1081 agggtgaagc ggggcaagat cggcaccaac atggctgtga gcaagacccc cagcggtagt
     1141 tgcaccagtc tattggcagt caccagggcc gtccagccac cttcccccat ctgcgaggtg
     1201 aaaaagacat ccacgtagct gtatatgtaa ccgatggaag tagaaatggt ggccggccag
     1261 agcatgtgca agtattcttt catgcccggt tccatcttac tgaagagctt taagtctcga
     1321 aaggtgggat gggctttgag catgcccggc atttgtacga ggaattgacc gatggctccg
     1381 gccagagtac cggcggccag gcagagaccg gtttcgccgc tacccttgaa accgaggcag
     1441 gcaatgatga tgcagagtga tgctactgct ggtgccatgg acggccagaa aaactggttg
     1501 taggtattag atatgccgca gccaatgcct actaatcctg caattaagat gagcggcgac
     1561 ataattctca gctgcatagt ggtctcgtgc cagagtttta ttttctctgc ttcactgccg
     1621 ctgcccggtg ctactaacgg tactatatac ggtgccaggg cgaaaactat gagagcgaga
     1681 agccctgtaa tcagtcctgt ccagacaagc agttgcacgg tcagcctgcc gcagtcgccg
     1741 gagtctttgc ggggagtcaa gacggctacg gccgaagaat gaaagggacc tcccagaccg
     1801 ccgaagagca ccagaatatt gccggtgaag agagtggcta tattgtaggc atcagcgatc
     1861 atggtggtgc caaaggcgct tgccaccacc atatctcgca agagaccggc gaacttggag
     1921 agaacggtaa agagggctac cagagaaaat gtcttacc
//
LOCUS       DAOQNZ010000836         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39810_length_1954_cov_3.123223, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000836 DAOQNZ010000000
VERSION     DAOQNZ010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39810_length_1954_cov_3.123223"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PKW73_13925"
     CDS             <1..1025
                     /locus_tag="PKW73_13925"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNA74439.1"
                     /translation="GNISQRGQSIVVKTGSGIIVGASGELEIAELPEGLVAKPSLLWK
                     LECDKAGAHNTEISYQTQGMNWKCDYVAVANADDSSCDLTSWVTIDNKSGATYKNAAL
                     KLMAGDVHKVQQPSAAPMMERAMAMDAVAAEPQFSEQSFAEYHLYSLKGRTDVRDNET
                     KQLTLFNASAVPVKKLYVLESNNSYYGGDGQQKQKVNVKLELANSKDNNLGMPLPKGK
                     VRVYKKDQDGALQFVGEDEIDHTPKDEKVRVYIGDAFDIAAERVQTGQQQISERVQRQ
                     SYSISLRNHKKEAVTVTCVEHAWGDWKIVNSSMPYTKKDSHTFEFNVKVAPDTEEKLT
                     YTIEIK"
     gene            complement(1039..>1954)
                     /locus_tag="PKW73_13930"
     CDS             complement(1039..>1954)
                     /locus_tag="PKW73_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNA74440.1"
                     /translation="GRVVSEPRQIPTPTGADNIINGVIELVRSFQADCQQVTGVSPMG
                     IAGVGIATAGIVDCNSGDIIGSTGNLPGWTGTSLKRVIETQILLPVHVDNDANAAAYG
                     EASARGLMHSNCVCTVTLGTGIGGGLVINGKMFRGAHWGGGEVGHMRLNLGNKRLCTC
                     GLFDCWEAYGSGRGLVTTGRDILAGLNADQTPLAKDPEALTTHAIVAAAKGGDIVAQR
                     ILHMWHEHIAAGIASLAHILDPDVFVVTGGLADCVDFPLLTEMVVDRVLPRIGDNLQL
                     HKSELEGLAGIIGAAQLVLDNLLDRIVA"
BASE COUNT          479 a          495 c          565 g          415 t
ORIGIN      
        1 acgggaatat ttcccagcgt ggacagtcga tagttgtgaa aaccggcagt ggtatcatag
       61 ttggtgcctc aggcgaattg gagatcgcgg agttgccgga aggtctcgtc gcaaaacctt
      121 ctttgctctg gaagcttgag tgcgacaagg ccggtgctca caataccgag atcagctacc
      181 agacccaggg catgaactgg aagtgcgatt atgtagcagt ggccaatgcc gatgattcca
      241 gttgtgatct gacaagttgg gtaaccatcg ataataagtc cggtgctacc tacaaaaatg
      301 ctgctctcaa attgatggcc ggcgatgtgc ataaggtgca gcagccaagt gctgctccca
      361 tgatggagcg ggccatggcc atggatgctg tggcggccga accgcagttt tccgagcaga
      421 gttttgccga gtatcatctg tatagtttga aaggccgcac cgatgtgcgg gacaatgaaa
      481 ctaagcaatt gacgcttttc aatgcctcgg ccgtgcctgt caagaaactc tacgtgctgg
      541 agagcaataa cagctattac ggcggcgacg gacagcagaa gcagaaagta aatgtgaagt
      601 tggaactggc caactctaaa gacaataatc tcggaatgcc gctacctaaa ggcaaggtgc
      661 gcgtctataa gaaagaccag gacggggctt tgcagtttgt cggcgaggac gaaattgatc
      721 acactcctaa agacgaaaaa gtgcgagttt atatcggtga cgctttcgac atcgctgccg
      781 agcgggtgca gacagggcag cagcagattt cagagagagt gcagcgtcag agctacagta
      841 tcagcctgcg caatcacaag aaagaagcag ttaccgtaac ctgtgtcgag catgcctggg
      901 gagattggaa aatcgtcaat tccagtatgc cttacacgaa gaaagactcc cacacctttg
      961 aattcaatgt gaaggttgct cccgatacgg aagagaaact gacctacacc atcgaaataa
     1021 agtaagaggc tcagcccttt aagctacgat tctgtcgagc aggttgtcga gaaccagttg
     1081 ggcagcgccg atgatgccgg ccagaccttc cagttcggat ttgtgcagtt gcaggttgtc
     1141 gcctattctc ggcaagactc ggtctacgac catttctgtc aaaagcggga agtccacgca
     1201 atcggccagc ccgcctgtga ctacgaagac atcggggtcg agaatatggg ccaggctggc
     1261 gatgccggcg gcgatatgtt catgccacat gtgcaggatg cgttgagcga ctatgtcacc
     1321 gccctttgcg gcggcgacga tggcatgggt ggtcagtgct tccgggtctt ttgccagggg
     1381 agtttgatcg gcgttaaggc cggccagaat gtctctgccg gtggtgacaa gaccacgacc
     1441 ggagccatag gcttcccagc agtcgaaaag tccgcaggta caaagtctct tgttgcctag
     1501 attgagacgc atgtgtccaa cttcgccgcc gccccagtgg gctcctctaa acatctttcc
     1561 gttgattacc agaccgccac caataccggt accaagtgtg acggtgcaga cgcagttgga
     1621 atgcatcagc cctcgggcgc tggcttcacc gtaggcagca gcgttggcat cgttgtctac
     1681 gtgaacgggc agcaaaatct gggtctcaat aacacgcttc aaggaagtac cggtccagcc
     1741 cggcaagttg ccggtggagc cgatgatgtc gccggaattg cagtcgacga taccggcagt
     1801 ggctatgcct acgccggcta tgcccatggg ggaaacgccg gtcacctgct ggcagtcagc
     1861 ctgaaaacta cgcaccagct caataacgcc gttgatgata ttgtcggcac ccgtgggcgt
     1921 gggtatttgc cttggctctg atacgacgcg cccc
//
LOCUS       DAOQNZ010000837         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39851_length_1953_cov_3.758693, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000837 DAOQNZ010000000
VERSION     DAOQNZ010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39851_length_1953_cov_3.758693"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKW73_13935"
     CDS             <1..542
                     /locus_tag="PKW73_13935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNA74441.1"
                     /translation="REGNPALTFEELSKPRAAGVSGNKNNIDALMAAQEVKPGNFLIL
                     PNMQLPWQDNTREYKKRFAHKNEAVFKGQFAILDKLLALNESLGIETLIVNMPVTPMN
                     MKLMPKGSYESYSAALNTVAQKRQVKVLDLNGTFDMSCFRDTVHMNGKGGAALLQAIA
                     ATLSEDKREQLSGKAPLPF"
     gene            546..1526
                     /locus_tag="PKW73_13940"
     CDS             546..1526
                     /locus_tag="PKW73_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74442.1"
                     /translation="MSQKELKAEENNDQEQEERARSAAYTLGKELNEEQVAWLEENLK
                     QEPQDLISRLELIGYYQFQSREEGQKQWCRHILALIENHPDLTGYAAAIVMNAGRKMS
                     PEQFAEARHLWQKKTEEHPDNGAVHGNAGAFLLFADLETADRLLDRACLLEPFNVRWP
                     AHLSLFHYMAFKKAGEKAEYHAERVVKYGRQSVDLGSPTPWLDLEHMGECALAKGNPQ
                     EARAFAAELSNLNIHATHRQAANAITGLAALQEGQKETAVALLLAKEEGFTSHRLTWK
                     LASALLHEQEREAVSVCINQYRQPLGKLADQWLAALEKGSTPDFRQAEEN"
     gene            1558..>1953
                     /locus_tag="PKW73_13945"
     CDS             1558..>1953
                     /locus_tag="PKW73_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74443.1"
                     /translation="MRWLLADTGNDFERRRKEQKQEQIERFWQRFAQEEKALARAARI
                     GNDAYLSSWLGENLLSLSEDILWELENISAAVSMQKSPMTESYTKLVITSGGRARARL
                     LVNHIVAAAPNLPSWQFAAYKEPNQFGQYQ"
BASE COUNT          561 a          538 c          501 g          353 t
ORIGIN      
        1 cccgtgaggg caaccccgcc ctcacattcg aagagctatc caagcccaga gccgccggcg
       61 taagcggcaa caaaaacaat atcgatgcgc tcatggccgc ccaggaagtg aagccgggca
      121 atttcttgat cttgcccaat atgcaactgc cctggcaaga caacaccagg gagtataaga
      181 aacgcttcgc ccataaaaac gaagctgtct tcaaagggca gtttgccata ctcgataaac
      241 tgcttgccct caatgaaagt ctgggaatcg agaccctcat agtcaatatg ccggtcacgc
      301 ccatgaacat gaagctcatg cccaaaggct cttacgaaag ctacagcgcc gcactgaata
      361 cagtggcaca aaagcgccag gtgaaagtgc tggatttaaa cggcaccttc gatatgagct
      421 gcttccgcga cactgtacac atgaacggaa agggaggcgc cgccttgctg caggccatag
      481 ccgccacctt gagcgaagac aaaagagaac aactttccgg caaagcgccg ctaccctttt
      541 gaagtatgtc tcaaaaagaa ttaaaagccg aagaaaataa cgatcaggag caggaagaaa
      601 gagctcgctc ggccgcctac accctcggta aagagctgaa cgaagaacag gtagcctggc
      661 tggaagaaaa tctcaagcag gaaccgcaag acttaataag ccgcctggag ctaataggct
      721 actatcagtt tcaaagccga gaagagggac aaaagcagtg gtgccgacat attctagcct
      781 tgattgaaaa ccacccggac ctgaccggct atgccgccgc catcgtcatg aatgccggtc
      841 gcaaaatgag cccggaacaa tttgcagaag cccgccatct ctggcaaaag aaaaccgaag
      901 aacaccccga taatggtgct gtgcatggaa atgccggtgc ctttctgctc tttgccgatc
      961 tggaaactgc cgatcgcctg cttgaccggg cctgtctcct ggagcccttc aatgtgcgct
     1021 ggccggcaca cctcagcctt ttccattaca tggccttcaa aaaagccggc gagaaagccg
     1081 aataccacgc cgaaagagtg gtcaaatatg gtcgccaatc ggtagatctg ggctccccca
     1141 caccctggct ggatctggaa catatgggcg agtgcgccct cgccaaagga aacccgcaag
     1201 aagcgagagc ttttgccgcc gaactttcca atttgaatat acatgccact catagacagg
     1261 cggccaacgc cataaccggt ctggcagcgc ttcaagaagg gcagaaagag actgcagtag
     1321 cgctcttgct ggccaaggaa gagggcttta ccagtcatag actgacctgg aagttagcct
     1381 ccgccctttt gcatgagcaa gaaagagaag ccgtaagcgt ttgcatcaac caatatcgtc
     1441 agcccctcgg taagctggcc gaccaatggc ttgctgctct ggagaagggc agcactcctg
     1501 actttcggca agcagaagag aactaagcaa agtagaccgt acggaaagga ataatttatg
     1561 cgctggctcc tggccgacac cggtaacgac ttcgaacgcc gtcgcaaaga acaaaaacag
     1621 gaacagattg agagattctg gcagcgtttc gctcaagaag aaaaagctct ggcgagagca
     1681 gcacgcatcg gcaacgacgc ctatctgtcc agttggctgg gggaaaacct attgtccttg
     1741 agcgaggaca ttctctggga attagagaat atctccgcgg ccgtcagtat gcaaaaatca
     1801 cctatgacgg aaagctacac aaagcttgtc atcacaagcg gtggcagagc cagggccagg
     1861 cttctggtca accacatcgt ggcggcagcg cccaacttgc cgagctggca gtttgccgcc
     1921 tacaaagaac ccaatcaatt cgggcagtac cag
//
LOCUS       DAOQNZ010000838         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39942_length_1951_cov_3.457806, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000838 DAOQNZ010000000
VERSION     DAOQNZ010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39942_length_1951_cov_3.457806"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKW73_13950"
     CDS             complement(<1..548)
                     /locus_tag="PKW73_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74444.1"
                     /translation="MVTRLLRFSAALFLVSIVSVNFAIAKTESEQIAELKEKAQFLSN
                     KKKFKEAIAESEQALKLNSRDASIYYLRARIRESQKDPLAAMKELNKAIELNPKYAEA
                     YLLRAKGYYFLAEVDEGRDNIARAKRDLDTALKLNPQLAEGHDFKGVGLANEDKPLEA
                     LIHFNKAVQLDKSSSRYLEHRA"
     gene            complement(793..1518)
                     /locus_tag="PKW73_13955"
     CDS             complement(793..1518)
                     /locus_tag="PKW73_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74445.1"
                     /translation="MGFLSGSRRVVNGKDLILVFSTLALVLSGFSTATAAQSTLKGGV
                     RLNNSVMRLERPVQSRSVSPENVTTDAIPVAPSRFSGRATAAGASGGLVDPKLFAATP
                     ARDNVPLSGQAENRRFDIGAERGSKALTLAWEAWHKQLCAEIYARWQEVALIRGEATM
                     KVTVTRDRHVMAEITRSSGKTSFDRILVRVINSLEGNPGLSFPAGSQRREVSFEADYV
                     ADTNVRGGYSWTKNDYETVRVDH"
     gene            complement(1620..>1951)
                     /locus_tag="PKW73_13960"
     CDS             complement(1620..>1951)
                     /locus_tag="PKW73_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74446.1"
                     /translation="PVAPAIARAASRPAPQPTYSEPAGTLTPFSNSRSEEFASGNFEE
                     RASGITSSAGYLDGRLKRVITYGLTGAAGAALVNSLSPGRSRSSMGNAALRGALVGSF
                     IGLVTGR"
BASE COUNT          495 a          517 c          489 g          450 t
ORIGIN      
        1 atggcccggt gctccaaata gcgagaagaa ctcttgtcga gttgaacggc tttgttgaag
       61 tgaattagcg cttctagagg tttgtcctca tttgcaagac cgacaccttt gaaatcgtga
      121 ccttcggcaa gctgaggatt gagttttagt gccgtatcaa gatctcgctt agcacgggcg
      181 atgttatcac gaccttcatc aacttcagcc agaaaataat agcccttggc tctgagcaaa
      241 taagcctcag catacttagg attaagctcg attgctttgt ttagttcttt catggccgcc
      301 aggggatctt tctgagattc ccttatgcga gcccgcaggt aataaatact tgcatccctt
      361 gaattgagct taagggcttg ctcgctttcg gcaatggcct ctttaaactt ctttttgttg
      421 gatagaaact gggccttctc tttaagctca gcaatttgct cgctctcggt cttggcaata
      481 gcaaagttta cggagacgat tgaaactaga aatagagcgg cagagaatct gagtaatctt
      541 gttaccataa gcgaatccac ttcaatctct cagccggact atccgatgta ctttgctaag
      601 gaccagtcaa attcatgacc tctaagccga tgaccgaact acaccgccat ctaaaaaaca
      661 gtcgaattct aacaggcggg cgacaacaga aacccactct caagatatca gcttattgtt
      721 tcgcaagaca agtagtaggc atcatgaaac tcactgggct tgcactttcg aaccttccaa
      781 acagcaaaga ctctagtgat ctacccgcac cgtctcatag tcgttcttgg tccaactgta
      841 accgcctctg acattggtgt cggccacata gtcggcttcg aagctgacct cgcgccgctg
      901 agagccggct gggaaggaga gaccgggatt accttcaagg gaattgatta cccgaaccag
      961 aatgcgatca aaagaggttt tgcctgagct tctggttatc tcagccatga catgacgatc
     1021 gcgagtaaca gtcaccttca tggttgcttc gccgcgaatc aaagccactt cctgccagcg
     1081 agcataaatt tcagcgcaca attgcttgtg ccaggcttcc caggccagag tcagggcttt
     1141 ggagccgcgt tcggcaccta tatcaaagcg ccggttctcg gcttgcccac taaggggcac
     1201 attgtctcgc gccggtgtgg cggcaaagag cttgggatcc accaagcccc ccgaggcgcc
     1261 ggccgcggta gcacgcccgg agaaacgtga gggagccaca ggaatggcat cagtggtgac
     1321 gttctcggga gaaacagagc gactttgcac cggtctttcc aggcgcatga cactgttatt
     1381 cagacgcaca ccgcctttca gtgtagactg tgccgccgtg gccgtggaaa agccggacag
     1441 aaccagagcc agcgtactga agaccaatat caggtcttta ccattaacaa cacggcgact
     1501 gccgcttaga aagcccatat acatttgccg atacccatac cgcttaagta tttcctgaat
     1561 aggaagacca taaattgaca ctttccggaa gacggcgact aacaccgtct tgttccttat
     1621 tatctaccag tgacaagccc gataaaagag cctacgagcg ccccccgcaa agcggcattg
     1681 cccatgctgg agcggctgcg gccaggagaa agggaattta caagggcagc gccggcggct
     1741 ccggtaagac cataagtaat aactcgtttg aggcgaccat caagataccc tgcactactg
     1801 gtgataccag aggcccgctc ttcgaagtta ccggaagcga actcttcact tctggaatta
     1861 ctgaagggag taagagtacc ggctggttca ctgtatgtcg gctgtggcgc aggccgcgaa
     1921 gcagccctgg caatggcggg cgccacaggg g
//
LOCUS       DAOQNZ010000839         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_39955_length_1951_cov_2.716772, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000839 DAOQNZ010000000
VERSION     DAOQNZ010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39955_length_1951_cov_2.716772"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /gene="atpA"
                     /locus_tag="PKW73_13965"
     CDS             <1..1418
                     /gene="atpA"
                     /locus_tag="PKW73_13965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006096654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNA74447.1"
                     /translation="GIARIYGMEKAMAGELVEFDDEEKTMGMVLNLEEDNVGVVVLGE
                     GRKIFEGMEVRTTGKIASVPVGEALLGRVVNAIGQPMDGKGPINASETSPIEVKAPGI
                     VSRKSVHEPLMTGIAAIDGMIPIGRGQRELIIGDRQTGKTAVAIDAIINQKNQPNRPI
                     CIYVAIGQKNSNVGKIQKILEEHGAMEYTIIVNAPATAAPALQFLAPYAGVAMGEYFM
                     KKGMHALCVYDDLSKHANAYRAMSLLLRRPPGREAFPGDVFYLHSRLLERAAKLSDKL
                     GAGSLTALPIIETQAGDVSAYVPTNVISITDGQIFLETDLFYAGVRPAINAGISVSRV
                     GGSAQTKAMKSVAGKLRLDLAQFRELEAFAQFASDLDKSTQDQIRRGQKLTEVLKQPQ
                     YQPLSLAQQVSIIFAANKGILDDVETKNLGKFKAEWFKYLSSQAKDIEAKLTGGDKLS
                     DAEEQALVDQLNKFKTNFFKP"
     gene            1462..>1951
                     /locus_tag="PKW73_13970"
     CDS             1462..>1951
                     /locus_tag="PKW73_13970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNA74448.1"
                     /translation="MANLKAIRQRIKSVQSTQKITRAMRLVAAAKVRRAQMRVQAARP
                     FTQAVVRVLREVVAEVSPVDLQGLPLLHKHAQIKKVALIVLSSDRGLCGGYNTTVFRE
                     VINRITELQSQGKEVGLILVGLKAASFFRSFKVEKLKSYTLLPAIPTVEEAKLIAGYA
                     ADL"
BASE COUNT          475 a          587 c          488 g          401 t
ORIGIN      
        1 acggtatcgc ccgcatctac ggcatggaaa aagccatggc tggtgaactg gtcgaatttg
       61 acgacgaaga gaaaaccatg ggcatggtct tgaacctgga agaagacaac gtcggcgttg
      121 tagttctggg tgaaggcaga aaaattttcg aaggcatgga agtgcgcacc accggcaaaa
      181 tcgcctcggt gcccgtaggc gaagcgctcc tcggtcgcgt ggtaaacgcc atcggtcagc
      241 ccatggacgg caaaggtccg atcaacgcca gcgaaacctc ccctatcgaa gtgaaggctc
      301 ccggtatcgt ttccagaaag tcagtgcacg agccgctcat gaccggtatc gccgccatcg
      361 acggtatgat cccaattggt cgcggtcagc gcgaacttat catcggcgac cgtcagaccg
      421 gtaagaccgc cgttgccatc gacgccatca tcaaccagaa gaatcagcct aaccgcccca
      481 tctgtatcta tgtggctatt ggtcagaaaa actccaacgt cggcaaaatc cagaaaatat
      541 tggaagagca cggcgccatg gaatacacca tcatcgtcaa cgctcccgcc actgccgctc
      601 ccgctctgca gttcctcgcc ccttacgccg gtgtggccat gggtgagtac ttcatgaaga
      661 aaggcatgca cgccctctgc gtttatgatg acctttccaa acacgccaac gcttaccgcg
      721 ccatgtccct cctgcttcgt cgtccgccag gtcgtgaagc tttccccgga gacgttttct
      781 atctgcacag ccgtcttctg gaacgcgccg ccaagctctc cgacaaactg ggcgccggct
      841 ccctcaccgc tctgcctatc atcgaaaccc aggccggtga cgtgtccgcc tacgttccca
      901 caaacgttat ttccatcacc gacggccaga tcttcctgga gaccgacctc ttctacgccg
      961 gtgtcagacc cgctatcaac gccggtatct ccgtaagccg cgtgggtggc tccgctcaga
     1021 ccaaagctat gaagtcggtg gccggtaaac tgcgtctcga ccttgcccag ttcagagaac
     1081 tggaagcctt cgcccagttc gcttccgacc tcgataaatc tacccaagat cagattcgtc
     1141 gcggtcagaa actgacggaa gtgctgaagc agcctcagta tcagcccctc agtctggctc
     1201 agcaggtttc catcatcttc gccgccaaca aaggcatcct ggatgatgta gaaaccaaaa
     1261 acctcggtaa attcaaggcc gagtggttca aatacctctc ttctcaggcc aaagatatcg
     1321 aagccaaatt gaccggaggc gacaagcttt ccgatgctga agagcaagcc cttgttgatc
     1381 aattgaacaa gttcaagacc aacttcttca agccctaatt agggtccatc aactggttat
     1441 acaagcaagt aggaagacga aatggctaac ctcaaggcaa ttcgacaaag gatcaaatcc
     1501 gtacagtcaa cacagaaaat aacccgcgcc atgcgtctgg tggcagccgc caaagtaagg
     1561 cgggcccaga tgcgtgtgca ggccgccaga cccttcaccc aggcagtcgt aagagtgctg
     1621 agagaagtcg tggccgaggt tagccccgtc gacttgcaag gcttgccgct cttgcataag
     1681 cacgctcaga tcaagaaagt ggctctgata gtcctttctt ccgaccgcgg tctgtgcggc
     1741 ggctataaca ccacggtctt ccgcgaggtt attaaccgca tcaccgaact gcagtcccag
     1801 ggtaaagaag taggcctcat tttggtgggt ctgaaagctg cctccttctt ccgctccttc
     1861 aaggtggaaa agctcaagag ctacacactc ttgccggcta tccccacggt tgaagaagcg
     1921 aagttgattg ccggctatgc tgctgacctc t
//
LOCUS       DAOQNZ010000840         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40025_length_1949_cov_3.242872, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000840 DAOQNZ010000000
VERSION     DAOQNZ010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40025_length_1949_cov_3.242872"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKW73_13975"
     CDS             complement(<1..666)
                     /locus_tag="PKW73_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74449.1"
                     /translation="MGYGTKWLISPVVDLTFISGGALWVLFGLHAVALAHCPVAFPLK
                     VLENIANIGVLFIAEAHTLSTFFLVSDEVEAGSQSSGLCPPQLASRISAVWSVPAMLF
                     LSVLVVGYVCLQFVVVPAVLAKIYLLLVPQHFIAQSRGVFLLYCLRCQYSLCGFRRLL
                     LFSTSRLVMFYWMSVLLSHWSSPSPVFLFQKLPDWSVLPDVVPAVLYLLSLVLGVAGG
                     IYSI"
     gene            complement(676..1023)
                     /locus_tag="PKW73_13980"
     CDS             complement(676..1023)
                     /locus_tag="PKW73_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74450.1"
                     /translation="METVISGVAQTGPVRVNNLAMLEALFNIGANAIEILLIALGALL
                     LMTALYRRHCAIRSLAAMSAPGRVPEDLVRFGSGSCYGKVLLGFSLVILGPYVPLWNK
                     VFWNCVQNDCWFR"
     gene            complement(1055..1393)
                     /locus_tag="PKW73_13985"
     CDS             complement(1055..1393)
                     /locus_tag="PKW73_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74451.1"
                     /translation="MENTIVSGVNTAGTVRVNMLANLETLLNMFANGTELIMIAFGVL
                     LIIFAFYQMYRARQVAASRLRPATVAILPWRLCTTRLYAGGAVIGFGLAVPGALNWLV
                     AFARDYALFN"
     gene            1626..>1949
                     /locus_tag="PKW73_13990"
     CDS             1626..>1949
                     /locus_tag="PKW73_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74452.1"
                     /translation="MNTRTLLTGLLTTSMLLLNAAKAQARDELVLTDRSQGCTDSKSV
                     VTTFNKVGDNYIEANKNGRGKVLRRKQLLDLVTAIEKARSQSTFNPESLGITAQSVKL
                     HTIEMQ"
BASE COUNT          611 a          550 c          475 g          313 t
ORIGIN      
        1 aatcgaataa atccctccgg ccacccccaa caccagcgaa agcagataaa ggacagccgg
       61 caccacatcg ggcaaaacac tccaatcagg cagcttttga aagagaaaca ccggcgaagg
      121 gctcgaccag tgtgatagca ataccgacat ccaatagaac atgacaagcc ggctggtcga
      181 gaatagaagc agccgccgaa agccgcagag agaatattgg cagcgcaggc aatagagaag
      241 aaaaacaccc cgactctgag cgatgaagtg ctgcggcaca agtagcagat agatcttagc
      301 cagcaccgcc ggcactacca caaattgcaa acacacataa ccaacaacga gtacggaaag
      361 aaacaacatg gccggcaccg accagactgc acttatcctg gaagcaagct ggggcgggca
      421 caagccggaa gactgagacc ccgcttcgac ctcgtcgctc accaggaaga aggtgctcaa
      481 ggtgtgtgct tcagcaatga acaagactcc tatattggca atgttctcca gaactttgag
      541 agggaatgcc accggacagt gagccaaagc caccgcatgc agaccgaaca aaacccagag
      601 agcgccgccg ctgataaaag taagatccac caccggtgat atcagccact tcgtaccgta
      661 acccatggac ttgcctcagc gaaaccagca atcattttgt acgcagttcc agaagacttt
      721 gttccagaga ggaacataag ggccaagaat aaccaggcta aaaccaagca agaccttacc
      781 atagcaagag ccggaaccga aacgaaccag atcttccggc accctccccg gagcagacat
      841 ggcagccaga ctgcggatag cgcaatgcct gcggtacagg gccgtcataa gcagcagcgc
      901 accaagagca atcagcaaaa tctctatggc gttagcgccg atgttaaaga gcgcctccag
      961 catggccaga ttattgactc gcaccgggcc tgtctgggca acaccactaa taactgtttc
     1021 catcacagct ctcccgataa agaaaaacaa ccggctaatt aaagagggca taatcacggg
     1081 caaaggcaac cagccagtta agggcgccgg gaacggccag accaaaaccg atcacggcgc
     1141 cgccggcata gagcctggtg gtgcagagac gccagggcaa gatggccaca gtggcagggc
     1201 gcaagcgact ggccgccacc tgccgagcac ggtacatctg gtagaaagcg aaaatgatga
     1261 gcaagacacc aaaggcaatc ataattagtt cggtgccgtt ggcgaacata ttcaacaagg
     1321 tctccagatt ggcaagcatg ttgactcgca cggtaccggc agtattcacc cccgatacaa
     1381 tcgtgttttc catctaaacc tccttcccca aaggaaacta tgcggtagcc tcactaaatg
     1441 agccttccgg cagaaaccga cactggtaaa tcgaacaact ttcacacaaa tctatgtgag
     1501 cgccggtcct aaaacaaccg ggtttacact gaagggttat cagggcttga tgaattaccg
     1561 gcaccgggga aagtcctaaa ataggcatac cgcagccgct ggaagcaaac ccttgaggtg
     1621 ccatcttgaa tacacgaacc ttacttaccg gcttgctcac aacatccatg cttctgctca
     1681 acgcggcgaa agcccaggcc agggacgaac tggttctcac cgaccgctcc cagggctgta
     1741 ccgacagcaa aagcgtagta accactttca acaaagtcgg tgacaactac atagaggcta
     1801 acaagaacgg cagaggcaaa gtcctccgtc gcaaacaact gttggacctt gtgactgcca
     1861 ttgagaaagc cagaagccaa tcaactttca atccagagag cctgggaatt acagcgcagt
     1921 cggtaaaatt gcataccata gaaatgcaa
//
LOCUS       DAOQNZ010000841         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40144_length_1946_cov_3.991010, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000841 DAOQNZ010000000
VERSION     DAOQNZ010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40144_length_1946_cov_3.991010"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKW73_13995"
     CDS             <1..251
                     /locus_tag="PKW73_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74453.1"
                     /translation="AVLIEHHHHKNPALAILNGIFSGQGAASMEGCPGTGNSRKEQQE
                     GRYPKRLPIEFAENHLFQNKTPPEKFRTYTNSFYQKPN"
     gene            complement(297..>1946)
                     /locus_tag="PKW73_14000"
     CDS             complement(297..>1946)
                     /locus_tag="PKW73_14000"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74454.1"
                     /translation="EGKWRCSSKVTMVLEDKIETEELLESSGSSDSLVESESAPSTLV
                     QSVVYTLPDKYEIIEQVGRGGMGTVFKARHKDLNEIVAVKVVNPELLLKGGETTSKRF
                     LAEAKAASRLKHPNLISLRDYGVTTDGAAYMVMDYAEGESLEQLLKRKGKLTVAEALP
                     IVLGICTGLIEAHENGLVHRDIKPANILLSPVPGSDGAVYCPRILDFGVARVTSGSET
                     QGLTGTGEIFGSPLYMAPEQGLSSKVDARADLYSLGCLLFEMLTGSPPYAGETAIQTV
                     MLHLSAPLPDADRVAGQTLPHGLGRLLNFCLQKSPDQRYQTVADLKRDLERVLSGKPA
                     LYEGVGSKQAGVKATRPKQFLVFALSAVISLLSLLFLFHSLQVEHGPSQPLNTAPVTG
                     IVKATTGGRLVDKALNMDSTKAFQAFNEGDYESSALMLKGAIVAYGDEIARLKIQKAG
                     KSGEAARKLEQEIDRLTGLMADDLHHVGDCFLRLGQKEEAMTNFAQAIKYYRLYALRG
                     WNNGLIDDTYEKHIALLRGAGRTKEAQVLTTELTEIRKSRH"
BASE COUNT          470 a          551 c          479 g          446 t
ORIGIN      
        1 gtgcagtgct gatcgaacat catcaccaca agaaccctgc tctggctatt ctcaatggaa
       61 ttttcagcgg acaaggagcc gcaagcatgg aagggtgccc aggcaccggc aatagcagga
      121 aggaacagca agaaggccgc tacccaaagc gcttgcctat tgaatttgcg gaaaatcatc
      181 tctttcaaaa taaaaccccg ccggaaaagt tcagaactta caccaattcg ttttatcaga
      241 aacctaatta agtttattag gttttgagcc ctaagtccaa tttgctccag gtttgcctag
      301 tgccgggatt ttctaatctc agtcagttcc gtggtcagaa cctgagcctc ctttgtacgg
      361 ccggcccctc gcaagagtgc aatgtgtttc tcgtaggtat cgtcaatcaa gccattgttc
      421 cagccgcgca gagcataaag gcggtaatac tttatggcct gcgcaaaatt tgtcatggcc
      481 tcctctttct gaccaagacg caggaagcaa tcaccgacat gatgcaagtc gtcggccatg
      541 agcccggtga ggcggtcaat ttcttgctcc agcttgcgtg ccgcttctcc cgacttgccg
      601 gccttctgta tcttcagcct ggcaatctca tcgccataag caacgatggc acctttcagc
      661 atcagggccg agctttcata atcaccttcg ttgaaagcct gaaaagcctt ggtggaatcc
      721 atattcaagg ctttatccac caacctgccg cccgtggtcg ccttgacaat accggtcacc
      781 ggtgccgtgt taagaggttg actgggcccg tgctcaacct gcaacgaatg aaagaggaag
      841 agcaagctta gcaaggaaat aacagcggat aaggcaaaga ccagaaattg ctttggacgg
      901 gtggccttta cgcccgcttg cttgctccca actccttcat agagtgccgg tttgccgctc
      961 agtacccgct ccagatcgcg cttcaagtcg gccacagttt gatagcgctg atcgggcgac
     1021 ttctgcaaac aaaagttcaa cagccgccca agcccatggg gcaaagtctg accagccaca
     1081 cgatcggcgt cgggaagcgg tgcactcagg tgcaacatca cggtttgaat ggccgtctcg
     1141 ccggcatagg gaggagagcc ggtgagcatt tcaaagagca gacagcccag tgaataaaga
     1201 tcggcccggg catcaacctt actgctgaga ccctgctcag gcgccatata gagaggactg
     1261 ccgaaaatct cgccggtacc cgtgagcccc tgagtctcgc tgccggaagt gacacgcgcc
     1321 acgccaaaat cgagtatccg tgggcaataa accgctccat ccgagccagg tacaggcgac
     1381 agcaaaatat tggccggctt tatgtcccgg tgcacaagac cgttttcatg ggcttcaatc
     1441 aagccggtgc agattcccaa tactatgggc agcgcctccg caactgtcag tttccccttc
     1501 ctcttgagga gttgctcaag gctttcacct tcagcatagt ccatgaccat gtaggccgca
     1561 ccatcggtag tgacaccata gtcgcgcagc gaaatcaagt tgggatgctt gagtcgactg
     1621 gcagctttag cctcggctag aaagcgtttg gaagtcgtct caccgccttt gagaagcaat
     1681 tcgggattga ctaccttcac ggccactatt tcattaaggt ctttatggcg agccttaaag
     1741 accgtcccca tgccgcctcg gcccacttgt tcgataatct cgtacttatc cggcaaagtg
     1801 tagaccacac tctgtaccag ggttgacgga gcgctttcgc tttccacaag ggagtccgag
     1861 gagccggaac tctctagcag ttcttcagtc tcaattttgt cttctaatac catggtcacc
     1921 tttgaagagc atcgccactt accctc
//
LOCUS       DAOQNZ010000842         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40155_length_1946_cov_3.174511, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000842 DAOQNZ010000000
VERSION     DAOQNZ010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40155_length_1946_cov_3.174511"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKW73_14005"
     CDS             complement(<1..186)
                     /locus_tag="PKW73_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74455.1"
                     /translation="MNNSPAPRERPRWRWASPLVLLLIILTWLSFAPAGAPGHAVKRF
                     ISFATGFCSSCARQEIDP"
     gene            complement(253..1017)
                     /gene="nadD"
                     /locus_tag="PKW73_14010"
     CDS             complement(253..1017)
                     /gene="nadD"
                     /locus_tag="PKW73_14010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNA74456.1"
                     /translation="MKPLKVGVLGGTFDPVHNGHVFVAEYVLQELGLDRVYLVTAKSP
                     PHKSHFIADAEFRHAMVEEAIKGKPRLLASRLELDREGKSYTVDTFRQLKEELPPGTE
                     LYMITSVEYLRPEPAESELAPADCLGKPADRHLHWLGNWEGVDELFRLCRIVVTPRRH
                     MDAALAREWAALLPAADIIVVDLPSPPVSSLMVKRHLKHGLPIHDLVPPAVEAWIKRT
                     GLYTADGSSADLAHLEQFAPSTETYSTERTLSPTSN"
     gene            complement(1111..1761)
                     /locus_tag="PKW73_14015"
     CDS             complement(1111..1761)
                     /locus_tag="PKW73_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74457.1"
                     /translation="MDTKLLDAEISRKLARQFGSKFIKILEDSPTLRRDLRKLHTLGA
                     RIQRWNGRDGAYCRSEPSHRRGTFIAIGSLCSPIMKAIYLAHEAYHALYGTTPNNPNP
                     TKISRKRYLSMGIAEETRAFLHQTKVANELWNSGNHVIPAWQMHLMLLYHTSGYTDMR
                     EHVLACRESIAFGTYEQNYGKIWDQAYRQFERERREKLAARRLRSLKSRNRLKQAA"
BASE COUNT          385 a          496 c          607 g          458 t
ORIGIN      
        1 ggggtcgatt tcctgcctgg cgcaagagct gcagaagccg gttgcaaatg atatgaagcg
       61 cttgaccgca tgaccagggg ctccggccgg agcaaaactg agccaggtca agataatcag
      121 taagagcaca agaggggagg cccagcgcca gcgaggcctc tcccgggggg cgggtgaatt
      181 gttcatttca gactttcaag aagaggaggg aattaaaatc cctccccggt ttaaagtcct
      241 ctttccgtct gttcagttgc tggtcggtga gagggttctt tctgttgagt aagtttcggt
      301 gcttggagca aactgctcca ggtgagccag gtcggcgcta cttccatcgg cagtatagag
      361 cccggtgcgt ttgatccagg cctctaccgc cggtggcacc agatcgtgga tgggaagtcc
      421 atgcttcaag tgacgcttga ccatcaggct ggacacaggc ggactgggca ggtccaccac
      481 gataatgtcg gctgcaggta agagtgcggc ccattccctg gcaagagcgg catccatgtg
      541 ccgacggggg gtgactacaa tgcggcagag acggaagagt tcgtctactc cttcccagtt
      601 tccgagccag tgcaagtgcc tgtcggctgg tttgccgagg caatcggccg gggcaagctc
      661 gctttcggcg ggctcgggtc tgagatattc gacactggtg atcatgtaga gttcggtacc
      721 cggcggcaac tcttccttca actgcctgaa ggtatccacc gtgtaagact tgccttcgcg
      781 atcaagctcg aggcggctgg cgagcaggcg gggcttgccc ttgattgctt cttccaccat
      841 ggcgtgcctg aattctgcat cggcaatgaa atgagatttg tgcggtgggc tcttggctgt
      901 gaccaggtaa acccggtcca gaccaagttc ctgcaagacg tattcggcaa caaaaacatg
      961 accgttatgt accgggtcga atgttccacc cagtactccg acttttaggg gcttcatgtg
     1021 aatgcctctt ccaagagggg ctcaagagca ccaccttcac tgaaggtggt gccgtcgagc
     1081 tgtgtttcta actttgggat tgcttgccgg ttaagccgct tgcttgaggc gattgcggct
     1141 cttcaagctt ctgagacggc gtgccgccaa tttttcacgg cgctcccgct cgaactggcg
     1201 ataggcctgg tcccagatct tgccgtaatt ttgttcgtag gtgccgaagg cgatagactc
     1261 tcggcaagcc agcacatgct cgcgcatgtc ggtgtagccg ctggtatggt aaagaagcat
     1321 caagtgcatc tgccaggctg gaatgacatg gttgccgctg ttccagagtt cgttcgctac
     1381 cttggtctgg tgcaggaagg cgcgagtttc ctcggctatg cccatggaaa ggtagcgctt
     1441 gcggctgatc ttggtggggt tggggttgtt gggagtggtg ccgtacaggg catggtaagc
     1501 ctcatgggcc aggtaaatgg ctttcatgat tggcgaacag agggagccga tggcgatgaa
     1561 agtgccgcgg cgatgactcg gctcggaacg acagtaggca ccgtctctac cgttccagcg
     1621 ttgaatcctg gcgccgagcg tgtgcaactt gcgcaaatca cggcgcaagg tggggctgtc
     1681 ttccagaatc ttgatgaatt tgcttccaaa ttggcgcgct agctttcggc taatctccgc
     1741 gtccaggagt tttgtatcca tggtgttctc cctgcctgat ggcgaaagag agttgataaa
     1801 acggaggtgc ggccgaaact ttattgctta gcggcagtca cctccaggtc atttgacaag
     1861 caagccgttt cccggctgga tgccggcagg ctctcccgct gggggatgct tgtgctggat
     1921 gctgtcttaa ggcaggcagt gcaatt
//
LOCUS       DAOQNZ010000843         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40382_length_1940_cov_4.475332, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000843 DAOQNZ010000000
VERSION     DAOQNZ010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40382_length_1940_cov_4.475332"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PKW73_14020"
     CDS             <1..1164
                     /locus_tag="PKW73_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNA74458.1"
                     /translation="NDEVKDALSDVHGTVVATLGDGRLKMLVVKCEKGKFAETEKKLT
                     ADKKHFTAVGRNYRFAANVVPNDPKFSSSWHLTAMNCPKAWDKGQGGAKIAVLDSGCQ
                     ASVADLNGKVEKGFDANTPAAKALGGILMASGSLPGVTDLAGAIAAEASSGANTDQNG
                     HGTWVASAAVAAMNNSQVTAGIAPSSTVYPIRIADAAKGSTAMTDDLSVACAMFKVFT
                     SGARIVNLSYGGPYYGFHNPVIHGPLHKIFMEYYYARQGLIFLSAGNDAIFDGTPNCP
                     YINMVSAIDSSGNLADFSNYGTIVDFTAPGKGIVVTDIDGLDATVNGTSFSCPIVAAV
                     ASLILSKNPALPNVAVEGILKASCVNSTPGWNPYFGWGMPDAKKAVDLATFGL"
     gene            1360..>1940
                     /locus_tag="PKW73_14025"
     CDS             1360..>1940
                     /locus_tag="PKW73_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74459.1"
                     /translation="MKFQARALIIASTLLGAMTSLAPAFADGMVSYPYDWVLELKTKP
                     LTRQFEYQGSTLKKIEKVLVEYHPAKDADTKTAYEFLWYNGGKALGMEKQRKFDLPQG
                     EGVAIRVSHMNGSSTEGENKAAANAILRLALDTYLNKNPIVQVRLPAETFNDITNHLV
                     SLGMQVAPEGVEDGAYSSNLTLFLYSEPDGNKKV"
BASE COUNT          454 a          536 c          489 g          461 t
ORIGIN      
        1 aacgatgaag tcaaagatgc tctctccgac gtacacggca cggtagttgc caccctcgga
       61 gatggacgcc tgaagatgct tgtggtcaaa tgcgagaaag gcaaattcgc cgagacagag
      121 aagaaactta ccgccgacaa gaaacacttc accgctgtcg gtcgcaacta tagattcgct
      181 gccaacgtag ttcccaacga tcccaaattt tcttcaagtt ggcatttgac tgccatgaac
      241 tgtcccaaag cctgggataa agggcagggc ggcgccaaga ttgccgtgct ggattccggt
      301 tgccaggcca gtgtggcaga cctgaacggt aaagtagaga aaggttttga cgccaataca
      361 ccggctgcta aagccctggg cggcattctc atggcctcgg gctctctccc cggcgttacc
      421 gacctggccg gtgccattgc ggcagaagcc tcttccggag ctaataccga ccagaatggt
      481 catggtacct gggtggcatc ggcggcggtg gcagccatga acaacagcca ggttactgcc
      541 ggtatcgctc ccagttccac tgtttatccg attcgtattg ccgatgccgc caaaggcagc
      601 accgctatga ccgatgacct ctccgtagcc tgcgccatgt tcaaagtctt cacctcgggc
      661 gccagaatcg tcaacctcag ctacggcgga ccctactatg gcttccacaa cccggtcatt
      721 cacggacccc tgcacaagat cttcatggaa tactattacg ccagacaggg tctgatcttc
      781 ctttctgccg gtaatgacgc catcttcgac ggcacgccca attgccccta catcaacatg
      841 gtttccgcca tagattcttc cggcaacctg gctgatttct ccaactacgg aaccatcgta
      901 gacttcaccg cccctggtaa aggcatcgtt gttaccgaca tcgacggact ggatgccacc
      961 gtcaatggta cttccttctc ctgcccgatt gtggctgccg tagcttccct catcttgagt
     1021 aagaacccgg ctctgcccaa cgttgctgta gaaggtatct tgaaagcaag ctgtgtcaac
     1081 tctactcctg gttggaaccc ctacttcggc tggggtatgc ccgatgctaa gaaagctgta
     1141 gacctggcta ccttcggtct ctagattagt ttacctgata acgtgaggga aggaagagga
     1201 gctcaaaagg ctcctcttct gtcattttct ctctttctta ttgagagagt cggtcctaat
     1261 tcctgcgctt tgtctgtttt agatccttgt cagaggctcc agtcaacctt ttagacggtt
     1321 atctgattga tagtagaaaa attttcgagg tacagtccag tgaaatttca ggcaagagca
     1381 ttgatcatag cgtctacatt gcttggggcc atgaccagcc tcgctcccgc cttcgcagac
     1441 ggaatggtct cttatcccta cgactgggtg ctggaactga agactaagcc cctgacacgg
     1501 cagtttgaat atcagggttc gaccctcaag aaaattgaaa aggtgctggt ggagtatcat
     1561 ccggccaagg acgccgatac taaaacagct tacgagttcc tctggtacaa cggcggtaag
     1621 gctctcggga tggagaagca gcgcaaattc gacctgcccc agggggaagg tgtggccatt
     1681 cgcgtcagcc atatgaacgg cagttccaca gagggtgaga ataaagctgc cgccaatgcc
     1741 attttgcgcc tggcccttga tacttatttg aacaagaatc ccattgtgca ggtaagactg
     1801 ccggcagaga ctttcaacga tattaccaac caccttgtct cccttggtat gcaagtggct
     1861 ccggaaggtg tcgaggacgg tgcttatagc tcgaacttga ctctctttct ctattcggag
     1921 cccgacggca ataagaaggt
//
LOCUS       DAOQNZ010000844         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40423_length_1939_cov_4.809979, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000844 DAOQNZ010000000
VERSION     DAOQNZ010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40423_length_1939_cov_4.809979"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKW73_14030"
     CDS             complement(<1..395)
                     /locus_tag="PKW73_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74460.1"
                     /translation="MMDTGAKRLELECQILCNALRQSWTASDLVGVVTAARQLAEFFP
                     DLLNANPSENNLPNLYEMLAVDIRTSPNRMKSLFRMRRKHHLRTQKLHGRENRLAFYE
                     LLDMGLIMRNARLRLSHDLNVLRQTLSSHG"
     gene            complement(714..1412)
                     /locus_tag="PKW73_14035"
     CDS             complement(714..1412)
                     /locus_tag="PKW73_14035"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNA74461.1"
                     /translation="MSTALVKYIQSELATLADPVKAEEMAAYMRTEMPFYGVQKPDRV
                     PLIREMKKRFPPVSLSDYQRNVLALWQLEHREEKYLAINYADSFKQYMIPQAVPLFER
                     LVREGQWWDFVDPIASWLVGRTLLSHEESLRPVIETYVQDEDFWIRRTALLAHLGHKK
                     MTNSEALFRYCTTLAGEKEFFIRKAIGWALREYSKSEPEKVSRYLKKNADNLSPLSYK
                     EGAKHLVKMGLLKL"
     gene            complement(1447..>1939)
                     /locus_tag="PKW73_14040"
     CDS             complement(1447..>1939)
                     /locus_tag="PKW73_14040"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNA74462.1"
                     /translation="NSKDQVGAIIDRLKLLPHPEGGYYRETYKCDRQISVSNGRERTV
                     GTAIYYLLTESTFSEMHRLRFDEIFHFYMGDAVEMLQLHEGQSTVHLLGTDLSNGQVP
                     QLLVPAGVWQGSRLVPGGQFALLGTTMTPGFDFADYEQGRRSELISAYPQQRSLIEYL
                     TRV"
BASE COUNT          496 a          513 c          474 g          456 t
ORIGIN      
        1 ccatgactgc tcaaagtctg gcgcaaaaca tttaaatcgt gactgaggcg tagcctggca
       61 ttgcgcatga tcaggcccat gtcaagcaat tcatagaaag cgaggcgatt ctcacgtcca
      121 tgcagtttct gagtgcgcag atgatgtttg cggcgcatgc ggaagagaga tttcatgcga
      181 ttggggctgg tcctgatatc cacggccagc atttcataaa gattgggcag gttattttcc
      241 gacgggttag cattcaaaag gtcggggaaa aactcggcca actgcctggc agccgtgact
      301 acacccacca gatcggaagc cgtccagctt tgcctgagtg cattacatag aatctggcac
      361 tcgagttcaa gcctcttagc cccagtatcc atcatgaaag tacgtcctcc acatccaaaa
      421 tttcaaaatg gggcaccggc tgaacaagcg caaaaaagga taccgggaat caaaaggttc
      481 ctcgaatccg actatcgtaa cccaatgagc cgccaaacgc caattaacga caaggagatt
      541 gtctaaaagc ttagtagagt cgccgagggg tctctccccg cttcctttag ccttcaataa
      601 accaaagtgg cccccgcaca gacagtcaaa gtgtttctat tttcgagcaa ctcacctaca
      661 tatacctcca cggcgcaaga gcattaattg agcgctctac cgggcgacaa ctcttagagt
      721 ttgagcaagc ccattttgac cagatgctta gcgccttcct tgtagctcag aggtgaaagg
      781 ttatcggcat ttttcttgag atagcggctg actttttccg gttcgctctt gctgtattcc
      841 ctcaaagccc agccgatagc cttgcggatg aagaattctt tctcaccggc aagagtagtg
      901 cagtagcgga aaagtgcctc actgtttgtc atcttcttgt gacccagatg agccaataaa
      961 gccgtgcgac gaatccagaa atcctcatct tgcacataag tttcaatgac tggacgcagg
     1021 ctctcttcat gacttagcag ggttcttccc accagccagg aggcaattgg atcaacgaag
     1081 tcccaccact gtccttcccg taccaggcgc tcaaagagcg gcaccgcttg cggtatcata
     1141 tactgtttaa atgaatcggc atagttgata gccaaatatt tctcttcccg gtgctcaagc
     1201 tgccagagcg ccagaacatt tctttgatag tcactgagag agaccggcgg gaagcgcttc
     1261 ttcatctcgc gtatgagggg cacgcggtcg ggcttctgca ccccatagaa aggcatttcg
     1321 gtgcgcatat aggcagccat ttcctccgcc ttcacgggat cggccagggt ggcaagttca
     1381 gactgaatgt acttaaccag ggcagttgac attgaaatca gacctttctt tagtgaagcc
     1441 ggtaactcaa actctggtca gatattcgat caggctgcgc tgttgcggat aagccgaaat
     1501 tagctcggag cggcgccctt gctcataatc ggcaaagtcg aaaccgggcg tcatggtggt
     1561 gccgagcaga gcaaattgac cacccggcac aagccttgaa ccctgccaga caccagccgg
     1621 tacaagcaat tgcggcacct gaccattaga gagatctgtg ccgagtaaat gtactgtgct
     1681 ttgcccttca tgcaactgca gcatttcaac ggcatcaccc atgtagaaat ggaagatctc
     1741 atcgaagcgc aagcggtgca tctcagaaaa agtcgactcg gtcaggaggt agtaaatagc
     1801 cgtgcccacg gtccgctccc gaccgttgga aactgaaatt tgccggtcac acttataggt
     1861 ttcgcggtag tagccccctt ccggatgagg cagtaactta agcctatcga taatggctcc
     1921 gacttgatct ttactgttt
//
LOCUS       DAOQNZ010000845         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40672_length_1933_cov_4.130458, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000845 DAOQNZ010000000
VERSION     DAOQNZ010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40672_length_1933_cov_4.130458"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1933)
                     /locus_tag="PKW73_14045"
     CDS             complement(<1..>1933)
                     /locus_tag="PKW73_14045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74463.1"
                     /translation="VVHENLDKRMALKVLVARRPDGHEFLRIQKEARALARFDHKNIV
                     RVFDVGVEGNVAYFSMEYLEGESLAQLLQKQGTIDPCLAFDIFSQLMEALEYAHGRGV
                     VHRDIKPANIMIVWQEGGTPLVKVVDFGIARYAKGEGSISLTRTGEIFGSPLYMSPEQ
                     ARGQRVDRRSDLYSSGLVLYEMLTGEKIFEGDSALLTIGKHICEDAAPILARQFDRNP
                     AIARLIGILLNKDKDDRYGSASAVLEDMRVLGLWPFDEGFLDSLDGAEGDGSDEGADD
                     QNQKRRRAFERSHRQALLRSVGALAEGRGLSLINSIDTPQWVLLLGFFALLCVVSVSV
                     ILFTVGGYGLKQPGLYEGYKAEKLLPSSYDDGQREKTQALSEYLTATTPFSHKERRGG
                     RNFRVFHFDEKISVGTIITQGKEYDARGTLVFPYVQWLEYRPSGNIAQFPQLLDRFAA
                     DDFCHVSLDHNFSVTTETVRHLAHMKSIMVLNLEATDVRNDVVEVFDQLPLLSALHIG
                     DTRLEPEAIAKWKGLHNLRVFKLSGDLECRRVLDMLAPCKGLEILGLGAIVVHRADIQ
                     RLAAFPKLFSLRFTAVDLDRDDMTSLAKMPKLRFLTIYEQEFGPEYAASLVEFPRLEA
                     FEGRMNNWPSSYPKMLKKIR"
BASE COUNT          469 a          574 c          478 g          412 t
ORIGIN      
        1 tctaatcttt ttgagcatct ttgggtaact gctgggccag ttgttcatgc gcccttcgaa
       61 ggcttcaagt ctggggaact ccaccaggct ggccgcatac tcgggaccga actcctgctc
      121 ataaatagtc aagaagcgca atttcggcat tttcgccaga cttgtcatat catcgcggtc
      181 gaggtccact gctgtaaatc taagggagaa gagcttgggg aaagcagcca gtctctggat
      241 atcagccctg tgaacaacga tagcgcccag ccccagtatt tccagacctt tgcagggtgc
      301 gagcatgtcc aggacgcgcc ggcattctaa gtctcccgat aatttgaaca ctctcagatt
      361 atgcaaaccc ttccacttag cgatggcctc cggctccaga cgagtatcgc ctatatgcag
      421 tgcggatagc agaggcagtt gatcaaacac ttccaccaca tcgttgcgca cgtctgtggc
      481 ctcgagattg agaaccatta tgctcttcat atgggcaagg tgcctgacgg tttccgtggt
      541 cacactgaag ttgtgatcaa gagaaacatg gcaaaaatca tcggccgcaa accgatccag
      601 aagctgcgga aactgcgcga tattgccgct tggtctatat tcaagccact gcacataagg
      661 aaaaaccagg gtgcctcgag catcgtattc cttaccttgc gtgatgatgg tgcccacgga
      721 aatcttctca tcgaagtgaa aaacccgaaa attccttccg ccgcgccgct ccttgtggga
      781 gaagggagtg gtagccgtga gatactcact gagagcctgg gtcttctctc tctgaccgtc
      841 gtcataagaa gaaggcaata gcttttcagc cttatatccc tcgtacaggc cgggctgttt
      901 caagccgtac ccacccacgg tgaacaggat gacactgaca ctcaccacgc agagcaaggc
      961 aaaaaatcca agcaaaagca cccactgcgg cgtatcgatg gaattaatca agctcagccc
     1021 ccggccctcc gccagcgccc ctactgaacg caaaagagcc tgccggtgcg atctttcaaa
     1081 ggcacgtcga cgtttctggt tctgatcgtc agcaccttca tcactgccat cgccttccgc
     1141 cccatcaagg ctatccagaa atccctcgtc aaagggccac aagcccagaa cgcgcatgtc
     1201 ctcaagcacc gccgaagcac tgccgtaacg gtcgtccttg tctttgttga gcagaatacc
     1261 gataagccgg gcgatagccg gattgcgatc gaactggcgg gcgagaatag gggcggcgtc
     1321 ttcgcaaata tgcttgccga tggtcaggag cgcggaatca ccctcaaaaa tcttttcccc
     1381 ggtcaacatc tcatacaaaa ccagcccgct ggaatagagg tcggaacggc gatcaactct
     1441 ctgtccgcga gcctgctccg ggctcatata aaggggactg ccgaaaatct cgccggtcct
     1501 ggtcagggaa atcgaacctt cgcccttggc atagcgggca atgccaaaat ctaccacctt
     1561 gaccagaggg gtgccgccct cctgccagac aatcatgata ttagccggct tgatgtcccg
     1621 gtgcaccaca cccctgccgt gagcatactc aagcgcttcc atcagttggc tgaagatgtc
     1681 gaaggccaga cacggatcta tggtgccctg cttttgcaag agctgcgcaa gactctcgcc
     1741 ctccaggtat tccatagaaa aataggcgac gtttccctcc acacccacat cgaagactcg
     1801 cacgatattc ttgtggtcaa aacgggccag ggccctggcc tctttctgaa tgcgcaaaaa
     1861 ctcgtgcccg tccggccgac gagccaccaa caccttgagg gccatgcgct tgtcgagatt
     1921 ttcgtgcacc acc
//
LOCUS       DAOQNZ010000846         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40706_length_1932_cov_6.795951, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000846 DAOQNZ010000000
VERSION     DAOQNZ010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40706_length_1932_cov_6.795951"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PKW73_14050"
     CDS             complement(<1..1255)
                     /locus_tag="PKW73_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA74464.1"
                     /translation="MPLQTAIHAAETERDLLGRPCIDGNTLTLGIWQEATSRTAKLLV
                     SSGLNIAQLRELTRKSPRPNKTYNFKMGAIDAQMSEALVHAIQAIATAPVPQDKVESH
                     AARPASISSEDLYRVLLAKDKILRQSLLGAGLTEKSLNQLEASLAASHHDEEADHSQT
                     GKPGKSSALKSFCTDLVTQAQEGKLSPVIGRLTEISKTIRVLGRKTKSNPVLLGEAGV
                     GKTAIVEGIAQRIATGNVPSKLKGKRILQLDLTAMLAGCQARGDFEARLVKLIKEVKA
                     AGNVILFIDEMHTLVGAGAAAGGLDAANSLKPELVRGAISVIGATTLTEYRKGVENRD
                     AALMRRFRPVLVEPPDINDTVQILQGLKAGLEKHHDLEICDQALVLAAHLAERYVADR
                     FQPDKSIDLVDEACSMVAIAHDQEKM"
     gene            complement(1322..>1932)
                     /locus_tag="PKW73_14055"
     CDS             complement(1322..>1932)
                     /locus_tag="PKW73_14055"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNA74465.1"
                     /translation="IENIHVWDFDIVEAHNIANQVYSQADIGRPKVEALKDLVKAHTG
                     MDLTIHNERVDGSQTLGEVVFLLTDTMSSRSEIWKKALRYKVGTKLMIETRMGADEGR
                     VYTINPTRPKQVRGWEGTLYGDDVAPVSACGTTISVGPTAEFISGLAVWQLIRWHAIE
                     QAKEKAAAASETAAQAVDTPVPLDHELVFGMQPAFLMSRQFE"
BASE COUNT          356 a          508 c          559 g          509 t
ORIGIN      
        1 ccattttctc ctggtcgtga gcgatagcca ccatggagca ggcttcatca acgaggtcga
       61 tgctcttgtc gggttggaaa cgatccgcta catagcgttc tgccaggtgg gcggctagca
      121 ccagcgcctg atcgcatatc tccaggtcat ggtgcttttc cagcccggct ttcagaccct
      181 gcaggatttg cacagtatca tttatgtccg ggggctccac cagcaccggt ctgaatcggc
      241 gcatcaaggc ggcgtctcga ttctccactc ctttgcggta ctcagtgaga gtggtagcac
      301 cgatgacgct gattgctcca cgtaccagct ccggtttgag ggaattggct gcatccagtc
      361 cgccggcggc cgcccctgca cccacgagag tgtgcatctc gtcgatgaat agaataacgt
      421 tgccggctgc ctttacttct ttgatcagct tcaccagtcg ggcttcaaag tcgccccggg
      481 cctggcagcc ggccagcatg gcagtcaggt ccaactggag gatgcgtttg cctttcagct
      541 tggaaggtac attgccggtt gcgattcgct gagcgatgcc ttccacgatg gctgttttcc
      601 ctaccccggc ttctcccaga aggacgggat tgcttttcgt tttgcgtcca agcacgcgga
      661 tggttttgct tatttctgtc agcctgccga tgactgggct gagcttcccc tcctgggctt
      721 gtgttaccag gtcggtgcag aagcttttca gagccgatga tttgcctggt ttgccggtct
      781 gagagtggtc cgcctcttca tcatggtgac tggcagccag agaggcttcc aactgattga
      841 ggctcttttc tgtcagaccg gcgcccagta gcgactggcg tagaattttg tctttggcca
      901 gcaggactcg atagagatcc tcgctgctga tggaagctgg cctggcagca tggctttcca
      961 ctttgtcctg gggcactggt gcggttgcta tagcttgaat tgcatgcacc agggcttcgg
     1021 acatctgggc atcgatagcc cccattttaa agttgtaggt cttgttcggc ctgggggatt
     1081 ttctagtaag ttctcgcagt tgggcaatgt tcaaaccact ggagacgagt aacttggcgg
     1141 ttcgggaagt tgcttcctgc catatgccaa gggtaagggt attgccatct atgcatgggc
     1201 gtcccagaag atctctttct gtttcggccg catggatggc agtttgaaga ggcatggtga
     1261 tatccatagg gcatactgaa tgggtcgggc gggtctgagc cgaagctcag accctggtta
     1321 gttattcgaa ttgtctggac ataagaaatg ccggttgcat accgaagacc agctcatgat
     1381 cgagaggaac cggtgtgtcg acggcttgtg cggcggtttc ggaagcagcg gcggctttct
     1441 ctttcgcttg ctcgatggcg tgccagcgaa ttaattgcca gactgccaga cccgagataa
     1501 attcagcagt gggtcccact gaaatagtgg tgccgcaggc tgataccggt gcgacatcgt
     1561 caccgtataa ggtgccttcc cagcctcgta cctgcttggg gcgggtggga ttgatggtat
     1621 agacgcgccc ctcatcggca cccattcttg tttcaatcat cagcttggtg cccactttgt
     1681 agcgcaaagc cttcttccag atttcactgc gtgatgacat ggtgtcggtg agtaagaaga
     1741 cgacctcacc cagggtctga ctgccgtcca ctctttcgtt atggatggtg aggtccatgc
     1801 cggtgtgggc cttgaccagg tctttcagtg cttccacttt ggggcgaccg atatcggcct
     1861 gactataaac ctggttggcg atattgtgtg cttctacgat atcgaagtcc cagacatgga
     1921 tgttttcgat gc
//
LOCUS       DAOQNZ010000847         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40790_length_1930_cov_3.968533, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000847 DAOQNZ010000000
VERSION     DAOQNZ010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40790_length_1930_cov_3.968533"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..664
                     /locus_tag="PKW73_14060"
     CDS             260..664
                     /locus_tag="PKW73_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74466.1"
                     /translation="MVINPLVNQMRGSSRHLPAKRRAGGLSSDAGRKPARLFFACLFT
                     LNLSCFCPLSLWQPLSPASFMESLTFCQPALAAPGIAENLLSGPDAAANNKQLTKSID
                     WLTSLPDAREKAAREGKLILWLHMLGNIDGFT"
     gene            689..1651
                     /locus_tag="PKW73_14065"
     CDS             689..1651
                     /locus_tag="PKW73_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74467.1"
                     /translation="MSLSQEPVFSYLRRRFVVGYRNIAGEPYAGDSGVHPVDGNATDT
                     TNGAGPHNLQIFVLAGDGTVITCLPGYWNKDDLLEELKLAEKLNALHCDRYIQSKEKA
                     ALAAALHLNHAKQHSEETTERSHLQGFDQRHIYEQRSTLADCIKDKELLQNADDKQIP
                     ARAFKTTDQIYHERMSKIGLVPYEKFDLAAYTNYGTSHYDRGESEASSHEKLALKGKS
                     LQALTRPTIVPTAAAAPSPAAQDLSLSQLNAAISRTPNRPELYETRALWFSKRGIPEQ
                     AHIDACRALWLGSRNQETLALKKALENYMAKRKASISALKARTN"
BASE COUNT          509 a          551 c          476 g          394 t
ORIGIN      
        1 acctgccctt ttactcgaaa ggacatccat aatcgactgg gcctcagagg gcacagaaag
       61 cgcgcctaca gaccatacag caagaacaag gcatagagct ttattcatca agactgacct
      121 ctagcttgca aaagaaaatc gagaagatcc gctctaagat gatccagcct accatcacaa
      181 taagatctct cacaagaaat ataacccctg aggggctctc tgtgttattc tcgtcttgac
      241 taccaggctt gggaggctca tggtaattaa tccactggta aaccagatgc gtgggagctc
      301 caggcatctc cctgcaaagc gtagggcggg cggtctcagt tcggacgccg gacggaagcc
      361 cgcccggctg ttctttgcct gcctgttcac tctcaacttg tcttgcttct gcccgctttc
      421 tctctggcaa cccctcagcc cggcctcctt catggagagc ctgacattct gccaaccggc
      481 gctggccgct cccggcatcg cagaaaatct cttgagcgga ccggacgccg ccgccaacaa
      541 caaacaactg accaagagca ttgactggct aacttctctt cctgatgccc gggaaaaggc
      601 tgctagagaa ggcaagctga ttttgtggct gcatatgctc ggcaatattg acggcttcac
      661 ctgaagcgcc ggcaatagcc tgagagccgt gtcgctctcc caggaaccgg ttttttctta
      721 tttgcgacgc cgctttgtag tcggctatcg caacatagcc ggagagccct atgccggcga
      781 ctccggcgtg caccccgtcg acggcaatgc aaccgatact acaaacggcg ctggtccgca
      841 caacttgcaa atatttgttc tggctggtga cggtacggtt attacctgtc tgcctggtta
      901 ctggaacaaa gatgacttat tggaagagct taagctggct gaaaagctca atgccctgca
      961 ttgcgatcgg tacattcaga gcaaagagaa agccgccctg gctgccgccc tgcatcttaa
     1021 ccatgccaaa cagcactctg aagaaaccac tgagcgcagt catttgcaag gcttcgatca
     1081 aaggcacatt tacgaacaga gaagcaccct cgctgattgc atcaaagaca aagagctgtt
     1141 gcaaaacgct gatgataagc agatacccgc cagggcattc aaaaccaccg accagattta
     1201 tcacgagagg atgagcaaaa ttgggctagt gccatacgag aagttcgatc tggcagccta
     1261 tacgaactac ggtacaagcc actatgacag aggtgaaagc gaagccagtt cccacgaaaa
     1321 gctggctctc aaaggcaaaa gtttgcaggc tttgacccgt ccaaccatag tcccgactgc
     1381 tgctgcggcg ccttcaccag cggctcaaga cctcagtcta tctcaattga acgccgccat
     1441 aagccgcacg cccaaccgac cggaactata tgaaaccagg gcactctggt tttccaaaag
     1501 gggcataccg gagcaagcgc acatcgacgc ctgtcgcgcc ctctggctgg gttcgagaaa
     1561 ccaagaaacc ctggcattaa agaaggctct ggaaaactat atggctaaaa ggaaagcttc
     1621 aatatcggct ctcaaagcaa gaaccaattg actgctgatt ctcttatcga cgggtaaaaa
     1681 ggatgcggca aattcattaa cagtgcccgg cagtagattg cgattggaac tgcaccgccc
     1741 gccttgcaga attacagtct caatgattca aaccgcggca aacaacggct gccagaagca
     1801 ctgctctcag cgaccgtgcc gccgtgcgca gccagttcca gcccaccaga gcacggtgcg
     1861 cttcctgact gaactgactg gcgagagcgc cgtgcagttg gtcggcaatg aagagtgtag
     1921 atagccagat
//
LOCUS       DAOQNZ010000848         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40841_length_1929_cov_3.369797, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000848 DAOQNZ010000000
VERSION     DAOQNZ010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40841_length_1929_cov_3.369797"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKW73_14070"
     CDS             <1..818
                     /locus_tag="PKW73_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74468.1"
                     /translation="VADLDPDMSERDVKYEALRIAEKAMEKAGAKTNRVKVSFADPIA
                     KGNFKEISFDTAQLVSYDKNLQSALYNIQINPVTAKIDIQALEVVDGAAKDERSLVLK
                     KLQGLDKQGVGIGPFIKQFFDIEQMVSNGNDDQAAEAVKKLTVSVQEQEARTKGAKAP
                     KPVAAAAASGTVLPVGKVYDQSYMNRNPDGLGGGWSVEDMVKAPEQYVKEKETQLGGK
                     ESADANPNFSKTLSWLYWNLVQNGKTAEAAKYGQRWMANQARYRFPEPKRAQK"
     gene            complement(826..1509)
                     /locus_tag="PKW73_14075"
     CDS             complement(826..1509)
                     /locus_tag="PKW73_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74469.1"
                     /translation="MSFQFPGRGLFALLLVPLLSACNQQNSAQSTGISLDVKPLEKDV
                     VYLEDRKDLAGRCKDLNFEAFTEWFYHCNVELAYDEIKKEDASGFGSCTLMIKGARVK
                     LACPVQVCVSKKAPADTLAHEEGHVEICRRVYAKAPEVAQKACQALMGKRFYGMGRTY
                     EAARKQALQLAYGEIAQAFRQRVTDVAEEASDRYDRLAARREGQKEWTVKVLIETALR
                     NTESGLGGP"
     gene            complement(1533..>1929)
                     /locus_tag="PKW73_14080"
     CDS             complement(1533..>1929)
                     /locus_tag="PKW73_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74470.1"
                     /translation="RDFAGKQRVELKDEPFNLDIVWHGWSQVKFTVFGEDGQPVPRCR
                     VSYTPYGEEPGDNSAPPSQIIGARSLGYPHGFVVTHDDGSCCLTLPSGVYSFKFLPPQ
                     AGSYAVRSIRQLSISADVNRKVTLELKEH"
BASE COUNT          522 a          531 c          503 g          373 t
ORIGIN      
        1 tggtggccga cctcgatcct gatatgagcg agagagacgt caaatatgaa gcgctcagaa
       61 tcgccgaaaa agccatggaa aaggccgggg ccaaaaccaa tcgggtcaag gtaagctttg
      121 cagaccccat tgccaaaggc aattttaaag aaattagctt cgacacggcc caacttgttt
      181 cctacgacaa gaacctgcag tcggctttgt acaacataca gatcaacccc gtcaccgcca
      241 agatagacat ccaggctctg gaagttgtgg atggagcggc taaagacgaa cgcagcctcg
      301 tccttaagaa gctgcaaggg ctcgacaaac agggcgtcgg catcggtccg tttatcaaac
      361 aattcttcga tatcgaacaa atggtatcga acggcaacga tgatcaggca gcagaagccg
      421 tgaaaaaact gacggtcagc gtgcaggaac aggaagcccg aaccaaggga gccaaggcac
      481 caaagccggt ggcagcggca gcagcttcgg gaacagtctt accggtaggc aaagtctacg
      541 atcagtctta catgaaccgc aatccggacg gactgggcgg cggctggtct gtagaagata
      601 tggtgaaggc gccggagcaa tacgttaaag agaaagagac acaactgggc ggcaaagaga
      661 gcgccgatgc caaccccaac ttttcgaaga ctctatcatg gctttactgg aacctggtgc
      721 aaaacggtaa gactgccgaa gccgccaagt acggacaacg ctggatggca aaccaggccc
      781 gctaccgctt ccctgaaccc aagagagccc agaaatagag agcactcacg gccctcccag
      841 accgctctcc gtattgcgga gagcggtttc tattagtact ttcacggtcc actctttctg
      901 cccctcgcgg cgcgccgcca acctgtcata acggtcggag gcttcttccg ccacatcggt
      961 aacacgctga cgaaaagcct gagcaatctc gccgtaagca agttgcagag cctgcttgcg
     1021 ggccgcctca taggttcgcc ccataccgta aaaacgctta cccatcaaag cctggcaggc
     1081 tttctgcgct acctcgggag ccttggcgta tacacgccgg caaatttcca cgtgcccttc
     1141 ttcatgggcg agagtatcag ccggcgcttt tttagaaacg cagacctgta ccggacaggc
     1201 gagttttacc cgagcgcctt tgatcatcaa ggtacaactg ccgaaaccgg aagcatcctc
     1261 cttcttgatc tcatcatagg caagttccac attgcagtga tagaaccact cagtgaaagc
     1321 ttcaaaattg aggtccttac agcgtccggc caggtccttg cggtcctcca aataaaccac
     1381 atccttttcc agcggtttca catccagact gataccggta ctctgagcag agttttgctg
     1441 gttgcaagcg ctgagcagag gcacaagcaa aagagcgaag agaccgcgtc ctggaaactg
     1501 aaaggacata aagccttaaa cctcaataga tgctagtgtt ctttcagctc gagagtaact
     1561 ttcctgttca catcggccga tatagacagt tgccgaatgg agcgcacggc ataggaaccg
     1621 gcctggggcg gaaggaactt aaaactataa actccggaag gcagggtcaa acagcaactg
     1681 ccgtcatcat gggtaaccac aaagccatgg ggatagccca gagagcgagc cccgattatc
     1741 tgtgagggag gggcggaatt gtcgcccggt tcctctccgt aaggcgtata ggagacgcgg
     1801 cagcgtggca ccggctgccc atcttctcca aagactgtaa atttgacctg ggaccagcca
     1861 tgccagacta tgtctaaatt aaagggttcg tccttcaatt cgactcgctg cttaccggca
     1921 aaatctcgg
//
LOCUS       DAOQNZ010000849         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40984_length_1925_cov_3.924599, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000849 DAOQNZ010000000
VERSION     DAOQNZ010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40984_length_1925_cov_3.924599"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /gene="argS"
                     /locus_tag="PKW73_14085"
     CDS             complement(<1..1598)
                     /gene="argS"
                     /locus_tag="PKW73_14085"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNA74471.1"
                     /translation="MIKEKLAEIVARAFESACAEGKMGELKECPVPIQIERPKLAEHG
                     DLACGVAMKLAAPLRSNPLKIAQTLAEHIGRDKEANGNRITSFAVAPPGFINFKLGSA
                     WLNQSLSRILIEGADYGKLDMGRGKRVLVEYVSANPTGDLHIGHGRNAVFGSCLVNLL
                     KFAGYEVEAEFYINDAGEQIVQLGRCAYALYRRLQGVEVEYPTEGYPEEYLSEFVAPI
                     VEREGKAFLSLSEEEGAVKLADLTMAEIIEHQKKLLAKLHVQFDRWYSERALHKRDQV
                     KQVLEEFLKHGFSYENEGALWLKTTELGDERDRVLRKSSGATTYLANDSAYHLDKYRR
                     GYDLMVNVWGADHHGQVPGLKGAMKALGEDPERLEVVLTQIVNLSRDGQIVRMSKRMG
                     TVVMLGEVMDEVGVDAVRYYLAESSPQNSINFDLELAKKTSRENPAFYIQYAHARCCA
                     ILRKALEPSQGQEGELPAPLTENQWQSFLQEYKTDPEVFESIFADSSEEVVQHQKALI
                     RRLELFPEEVREAVETRQPGRLAR"
BASE COUNT          435 a          590 c          463 g          437 t
ORIGIN      
        1 taccgggcca ggcgaccggg ctgcctggtt tccacggctt cccgcacttc ctcaggaaaa
       61 agctccagac gccgaatgag cgccttttga tgctgcacga cttcctccga gctgtccgcg
      121 aatattgact cgaagacctc gggatcagtc ttatactcct gcaggaaaga ctgccactgg
      181 ttctccgtca aaggcgccgg caactcgccc tcctgtccct ggcttggctc gagagccttg
      241 cgcaatatgg cgcagcaacg agcatgggca tattggatat agaaagcagg attttcccgg
      301 ctcgttttct tagccagctc caggtcgaaa ttaatggaat tctgcggaga agactcagcc
      361 aggtaataac gcacagcgtc cacacccacc tcatccatga cttcgcccag catcactact
      421 gtacccatac gctttgacat gcgtactatc tgcccgtcac ggctcaagtt gactatctgg
      481 gtgagaacta cctccagacg ctccggatct tcgcccagag ccttcatagc ccccttgagc
      541 ccgggaacct gaccgtgatg gtccgccccc cagacattca ccatcaaatc atagccccgt
      601 ctgtatttat ctaggtgata ggcggaatcg ttagccaggt aggtggttgc cccggaagac
      661 ttacgcaaga ccctgtctct ttcatcgcca agttcggtgg ttttaagcca gagagcgcct
      721 tcgttctcgt aactgaagcc atgcttgaga aactcctcca gcacctgctt gacttgatcg
      781 cgcttgtgca aggcccgctc cgaataccag cgatcgaact ggacatgaag cttggccaag
      841 agcttcttct ggtgttcgat aatttccgcc atggtcagat cggcaagctt gacggcgcct
      901 tcttcctcgc tgagagagag gaaagcctta ccctcccgct ccacaatcgg ggcaacaaat
      961 tcgctcagat actcttccgg gtagccctca gtcggatatt ccacttccac gccctgaagt
     1021 ctgcgataaa gagcataggc gcaacgcccg agctgcacaa tctgttcacc ggcatcatta
     1081 atgtaaaact ccgcctcgac ctcatatccg gcgaacttga gcaagttcac cagacaacta
     1141 ccgaaaacgg cattgcggcc gtgtccgatg tgcaaatcac cggtaggatt ggcggaaaca
     1201 tactccacca gcacccgttt tccccgcccc atatcgagct taccgtagtc ggcaccctca
     1261 attaagatgc gggacaaact ctgattgagc caggcactgc ccaacttgaa attgatgaaa
     1321 ccgggcgggg ccactgcaaa ggaagtgatt cgattgccat tggcctcctt gtcgcgacca
     1381 atgtgctcag ccagagtctg agcgattttc agaggattgg agcgcaaggg agcagccagc
     1441 ttcatggcta cgccacaagc caggtcaccg tgttcagcca gcttgggtcg ctcgatctga
     1501 atgggcaccg ggcactcctt cagttccccc atcttgcctt cagcgcatgc actctcaaaa
     1561 gccctggcca ctatttcagc cagcttttct ttaatcatga aatttccgtg ctccctgccc
     1621 agatctccag attggtcatg atattacatt ccaagtaccg atttgctcat cttgaagcaa
     1681 gcttgcagac ttccttgcaa attcccgctc ggcacaagcc cggagaggag gctcctatcc
     1741 accggcaacc gcaggagcag ctcaggcacc tgctatgatc tgagccgcca tatttcacct
     1801 gggagaaagc ctgtgaagaa tctaatgctc agttccgctt gggcccttgg actgtccgct
     1861 tcaatggcat ggacagccgg tcctggcgct agcgctgaag atcttagtta tgcaaccgat
     1921 ccggt
//
LOCUS       DAOQNZ010000850         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_40991_length_1925_cov_3.187166, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000850 DAOQNZ010000000
VERSION     DAOQNZ010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40991_length_1925_cov_3.187166"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKW73_14090"
     CDS             complement(<1..590)
                     /locus_tag="PKW73_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74472.1"
                     /translation="MKIRGQVFFLAVPLLCMVLPACVGLSPAQNQGLPPEPLFVIKVD
                     RDKSLAPSAVDDSSLDKFFSDEIEQPGLNRDRFDRIMADAIIKFDPTQELVSIKDHNI
                     SLKRSDGTTSSTFTDNAWAVCREIPGGRKRSLRMFLSSSKEIGRADFNKNSGINDVVP
                     LVRSTEMIEQTSALGASAQGAAAESLKLAWHPIAPG"
     gene            823..1806
                     /locus_tag="PKW73_14095"
     CDS             823..1806
                     /locus_tag="PKW73_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNA74473.1"
                     /translation="MKKNSVLNVVAVAFTVTLITAGCGENTQALKDRISDLESENAGL
                     KAQIAILKDTFAQAKTAELKAALQPMNDALANRNKDGSLNTGDALPNAAGQGGNATQA
                     APGATGNENKATVTFIDLEETPTADTIKDLARLGLFEGMGNKFEPNKPITRAEYIEWL
                     YKAFNILEPEAKRLRLAPQLAPIFKDVPASVPKYKYIQALANAGYSIGYEDGTFRPDK
                     PLTREEMLGIKVGVDVGKTLPPWRSQMDAVWKFSDGKDVSERFTGYLNQDFYVSGPKG
                     SNIHRAFGSIGAFKPKQPVLRYEAAATLWQMGQFGDTQTTNAGVLLKQSGR"
BASE COUNT          541 a          517 c          463 g          404 t
ORIGIN      
        1 attccgggag cgatagggtg ccaggccagc ttgagagatt cggccgccgc gccctgggcg
       61 gacgcaccca gagcgctggt ctgctcaatc atttcagtgc tacgcaccag aggcacaacg
      121 tcattgatac cactattctt gttaaagtcc gcacggccaa tctccttcga actcgatagg
      181 aacattctca ggcttctctt gcgcccacct gggatttccc ggcacacagc ccaggcgtta
      241 tcggtaaagg tactggaagt cgtaccgtcg gagcgcttca gtgaaatatt atgatcctta
      301 atggaaacta gctcctgggt tggatcaaac ttaattatgg catcggccat aattcggtca
      361 aaacggtcac ggtttaagcc cggctgctca atttcatctg aaaagaactt atccaacgat
      421 gagtcgtcaa ctgccgaagg ggcaagagac ttatcgcgat caaccttgat aacgaataat
      481 ggctcaggag ggagtccctg gttttgagcg ggcgataaac cgacacaagc cggtaacacc
      541 atgcacaata gcggcacagc aagaaaaaat acctggccac ggatcttcat agggcgaacc
      601 tcccacaagt gaaaaaagtg tagcgcaaac tacaactcaa gcggaacctg tgccggtggg
      661 aaatccaaaa ccacgaatgg aaaaactacg cttccgcctt gcatcattgc aacgttattt
      721 tcacaaacca gctaaaatct atgggtctat cgttatgcct gacaattgaa actcgtacgt
      781 tgccgcgtgg taaatgtaat caggtctagc tccggggggc ttatgaaaaa gaattcggtc
      841 ttgaatgttg tggcagttgc tttcacagtc acacttatca ctgccggttg cggcgaaaat
      901 actcaagctc tgaaagaccg catatcagac cttgaaagcg aaaacgccgg tctgaaagcc
      961 cagatcgcca tattgaaaga caccttcgcc caggccaaaa cggccgaact gaaagccgcc
     1021 ctgcagccaa tgaacgatgc cctagccaat cgcaataaag atggttctct gaacaccggc
     1081 gatgctttgc cgaatgcagc cggacaaggc ggcaacgcaa cgcaagcagc tccgggtgca
     1141 acaggcaacg agaacaaagc caccgtcacc ttcatcgacc tggaagaaac acctaccgcc
     1201 gacaccatca aagacctggc tcgtctgggg ctgtttgagg gtatgggcaa taagttcgag
     1261 ccgaacaaac ctataacaag agccgaatat attgaatggc tctacaaagc tttcaatatt
     1321 cttgagccgg aagccaagcg cctgcgtctg gcaccgcaac tggcaccaat cttcaaagac
     1381 gtaccggcca gcgttcccaa atacaaatac attcaggctc tggcaaatgc aggctattca
     1441 ataggctacg aagacggcac attccgcccc gataagcccc tcaccagaga ggaaatgctg
     1501 ggaattaagg ttggtgtgga cgttggcaaa accctgccgc cctggcgcag ccagatggac
     1561 gccgtgtgga aattcagcga cggcaaagac gtgtcggagc gtttcaccgg ctatctcaac
     1621 caggatttct atgtatccgg acccaaaggc tcaaacattc acagagcctt cggcagtatc
     1681 ggtgccttca aacccaaaca gcccgtactg cgctatgaag ccgctgccac tttatggcag
     1741 atgggacagt ttggcgatac gcaaactact aatgccggtg tgctcttgaa gcagagcgga
     1801 cgttaaacca cttgcaaaaa aaacgaaatc gccagcccag ggaaaaacac gctttaccta
     1861 ttttagtttt agcgccaata actcttttgc aatttttcct gttttgctgc caggtttaag
     1921 acttc
//
LOCUS       DAOQNZ010000851         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41023_length_1924_cov_3.635099, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000851 DAOQNZ010000000
VERSION     DAOQNZ010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41023_length_1924_cov_3.635099"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PKW73_14100"
     CDS             <1..1138
                     /locus_tag="PKW73_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74474.1"
                     /translation="AEQVAQDLLDEALEEKQGADDLVFARLCLAKAKRSNKKPAEAAK
                     LLLVSEESAKVLDEKNAEKTPARQYAVEQELAYCLAEQNENDKALAKLQKLLAWNKQF
                     NGGKSSQAQLLNWLGYCQLKMGKAQEAVASYNEAITLDPKFQNLYYWRANCYEQLKQS
                     AQALADYSKALELNAKDAGCLEERGNLYLKTGDYKNAALDLNAAVKLAPRDPDKYRSR
                     AVLHRLEKHYDLVVQDFDQSIRLNKSQPNPFDYIQRAQAKARLSQESAALADCDQAIK
                     LMPLLDTSYLAKADILMKFKKPDQALKVINQCLTSELYRTPPAQGWSEAAGGPDYAFV
                     LRKRAAILDSLGRSKEAAKDRQEATKVSKSADIQPFNAKYLFTD"
     gene            1203..1682
                     /locus_tag="PKW73_14105"
     CDS             1203..1682
                     /locus_tag="PKW73_14105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNA74475.1"
                     /translation="MTAFATIARKPLVALDPDFEAKIRASFEHQGLKRLGGELKEISL
                     GHVEAHLPYSDMVRQQHGLFHGGMVAMVADSASGYATYTVLAPDEECVSAEFKINFLQ
                     PARGEKIIARGGIIKVGRTLVITEATVSVIRDGQEIECALMLHTLARIKHMKSGKNT"
     gene            1724..>1924
                     /locus_tag="PKW73_14110"
     CDS             1724..>1924
                     /locus_tag="PKW73_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74476.1"
                     /translation="MPVNLCKLRGKLRVRLGSALLISLMSYAVPTTGALAVEEKDPYL
                     YLEEVQGKDALAFARRLNKQSTG"
BASE COUNT          561 a          507 c          494 g          362 t
ORIGIN      
        1 agccgagcaa gtggcacaag acttgctgga tgaagcgctg gaggaaaagc aaggagcgga
       61 cgacttagtc tttgcgcgtc tatgcctggc caaagccaag cgcagcaaca agaaaccagc
      121 tgaagccgcc aaactacttt tggtcagcga ggagtcggcg aaggtcctgg acgaaaagaa
      181 cgcggaaaaa acccctgccc ggcaatatgc cgtggagcaa gaactggcct actgcctggc
      241 tgagcagaac gagaacgaca aggctcttgc gaaactgcaa aaactactag cctggaataa
      301 gcaattcaat ggcggcaaaa gcagtcaagc ccaactcctg aactggcttg gttactgtca
      361 gttgaaaatg ggaaaagcac aagaggcagt ggcaagctat aacgaggcca ttacccttga
      421 tccgaaattt cagaatctct actactggcg ggccaattgc tacgaacagc taaagcagtc
      481 ggctcaggcc ctggctgact acagcaaagc attggaactg aatgcaaaag acgccggctg
      541 cctggaagaa cggggcaacc tctatctgaa aacgggagac tacaagaatg ccgctctcga
      601 cctgaatgca gccgtaaagc tagcccccag agaccctgat aagtatcgca gtcgcgccgt
      661 tctgcaccga cttgaaaagc actacgacct ggtcgtgcag gattttgacc agagcataag
      721 actcaataaa tcacagccca atcccttcga ctacatccag agagcccagg ccaaagcaag
      781 actgtcgcaa gaaagtgcag ccctggccga ttgcgaccag gccataaaac taatgcccct
      841 tctagacacg tcttacctgg ccaaagcaga catcttgatg aagttcaaaa aacccgacca
      901 ggccctcaag gtgatcaatc aatgcctcac tagcgaactt tatagaaccc caccggcgca
      961 gggctggagc gaagccgccg gtggtcccga ttatgctttt gtgctaagga agcgagcggc
     1021 tatcttggat agcctggggc ggagcaaaga agcggcgaaa gaccggcaag aggcgactaa
     1081 agtctcaaaa tccgcagata ttcagccttt caatgcgaaa tatctattta cagactgacg
     1141 gcccaatctg catgattgga tttggtacaa tcgcagaact caaccgggag tcccgaatca
     1201 aaatgacagc cttcgccacc atagctagaa aacccctggt cgcactcgat cctgattttg
     1261 aagcaaaaat tcgggcgagc ttcgaacatc aaggcttgaa acggctgggc ggtgaactta
     1321 aagaaatcag tctcggtcat gtggaagcac atctacctta cagcgatatg gtgcggcaac
     1381 aacatggtct tttccacggc ggcatggtag ccatggtcgc cgactcggcc tccggttatg
     1441 ccacctatac agtcttggct cccgatgaag aatgcgtctc cgccgagttt aagatcaact
     1501 tcctgcagcc tgccaggggt gaaaaaatca tcgcccgcgg tggcatcatc aaagtcggac
     1561 gcacccttgt cataaccgaa gccacagttt cggttatcag ggacggtcaa gaaatagaat
     1621 gcgctctcat gctgcataca ctggcccgca tcaagcacat gaagagcggt aaaaacacct
     1681 gaaccgcaag caaccgtaaa tcacaagtgg caagagaagg taaatgccgg tcaatctatg
     1741 taagttgaga ggcaagttga gagtgaggct aggctcggcg cttcttatca gtctgatgag
     1801 ctatgctgtt cccacaacag gagccctggc cgtggaagaa aaggaccctt atttatatct
     1861 ggaagaagtg cagggcaaag acgctcttgc tttcgcccgc cgcttgaaca agcaaagtac
     1921 cggc
//
LOCUS       DAOQNZ010000852         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41188_length_1920_cov_4.545845, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000852 DAOQNZ010000000
VERSION     DAOQNZ010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41188_length_1920_cov_4.545845"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PKW73_14115"
     CDS             complement(<1..1127)
                     /locus_tag="PKW73_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74477.1"
                     /translation="MKYLYPKDLANYITNHWQSLDKARDGKRATDSVWESLALPDKEA
                     LEDLLSTCYQASLMTHEGENVAFRLILADPDQFAAFEGLPEGVQKLEFADYLPFSTST
                     LRRLSPAASYSRSMIGIRFDAEVGLEIWGIIHTGPRWLHRSHGGRGAPPKLPPALVIK
                     VMGPGTLEVSRGNKTLVSLSEGKVLGQSLNVFSSAWLQEWFEPIRQERLELHRQASQG
                     TDEEWSELDPELTKIIDQQMMKRVIAAMRAFRHGGTLIFIPPDRAAELTRSNPYLTMK
                     YKFAEGEGRARFRTLIIEIMNTLASLPSANSHLVGWEEYQQTMDPRIIPLDESIFEMS
                     HLISSLSVADGAVVLTKRFEIIGFGAEIHCETTEVGTITKSL"
     gene            complement(1180..>1920)
                     /locus_tag="PKW73_14120"
     CDS             complement(1180..>1920)
                     /locus_tag="PKW73_14120"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74478.1"
                     /translation="FAEAKEQWKTSRQADDRAVKTLDKAIKLNPKRADYYNLKAQWLW
                     NLEDDDKLGLQSAEKSIALNPMAAYPWLLKAKFLGRLNRNEEALSAVNTAITLSPEAH
                     CYYDKAQLLKKMGHYKESEKNLDTALKLSPDDIDSLAVRADLRMQSKNYKGVLADTDA
                     ALKTNLAPMITIHRRMLLLRAEAYRQLKDYSKARKCYEDALKLFSSDRNVASSAQKFF
                     AEIGDKEMVQRLQAKLKQLDSDIVPLGM"
BASE COUNT          384 a          464 c          540 g          532 t
ORIGIN      
        1 agcgatttgg ttatggtacc gacttcagtg gtttcgcagt ggatttcagc tccaaagcct
       61 attatctcga agcgcttggt gagcaccacg gcgccatcgg ccacggagag gctggagata
      121 aggtgagaca tctcgaagat tgattcatcc aggggtatga ttctggggtc catggtttgc
      181 tgatattcct cccagcccac caggtggctg ttggctgaag gcaggctggc aagcgtattc
      241 atgatctcaa taatcaaggt gcgaaagcgc gctcgccctt ccccttcggc gaacttgtat
      301 ttcatggtca aatagggatt ggatcttgtt aactccgccg ctctatcagg cggaatgaag
      361 atcaaagtgc cgccgtgacg gaaggcgcgc atggcggcaa tgactcgttt catcatctgc
      421 tggtcgatga ttttggtcag ttccggatct agttcggacc attcttcatc ggtgccctgg
      481 ctggcttgtc tgtgcaattc aagacgttcc tgcctgattg gttcaaacca ttcttgcagc
      541 caggcggagc tgaaaacatt gagggattgt cccagcacct tgccttcgga aaggctgacg
      601 agcgttttat tgcctctcga tacttccagg gtaccgggac ccattacttt gatcacaagg
      661 gccggcggca gtttgggcgg ggcgccccga ccaccgtggc tgcgatgcaa ccaacgcgga
      721 ccggtatgga tgatgcccca gatttccagg ccgacttcgg cgtcgaaacg gatgccgatc
      781 atggatcttg aataactggc tgccggagag agtcttcgca gggtggaggt ggaaaagggt
      841 agatagtcgg cgaattccaa tttctgcacc ccttcaggca gcccttcgaa ggcggcaaac
      901 tgatcgggat cagccagaat caagcgaaag gcaacgttct caccttcatg ggtcatgagg
      961 ctggcctggt agcaagtgga gagcaaatct tccagggctt ctttgtcggg cagggccagg
     1021 gattcccaga cggaatcagt ggctcgcttt ccatctctgg ctttgtccag gctttgccag
     1081 tgattggtta tgtagttggc caggtcttta gggtacaggt atttcaattg gggctccgag
     1141 cttgggactc ttcagtttat tttagggcta atcttttccc tacataccca gagggacgat
     1201 gtcactgtct agctgtttga gtttggcttg cagtctttgt accatttctt tgtcgcctat
     1261 ttctgcgaag aacttttgag ctgagcttgc cacgtttcgg tcgctgctaa agagcttgag
     1321 agcatcttcg tagcatttgc gagccttact gtagtctttc aattgccggt aagcttcggc
     1381 gcgcagtaag agcatgcgtc tatgtatggt tatcattggt gccaggttgg ttttcaaagc
     1441 agcgtcggta tctgccagga cgcctttgta attcttgctc tgcatgcgca ggtcggctct
     1501 aacagccagg gagtctatgt catcaggtga aagtttcaga gccgtatcga gattcttttc
     1561 ggactctttg tagtgtccca tcttcttgag cagttgtgct ttgtcgtaat aacagtgcgc
     1621 ttccggactc aaggtgatgg ccgtattgac ggcgctcaga gcctcttcat tgcggttcag
     1681 gcgccccagg aacttggctt tcaaaagcca gggataagca gccatcgggt ttagtgcgat
     1741 ggatttttcg gcggactgta gaccaagttt gtcatcgtct tccaaattcc agagccattg
     1801 ggccttgaga ttatagtaat cagcgcgttt tggattgagc ttgatggctt tgtctagagt
     1861 ctttacggct cggtcatccg cctggcggct agttttccac tgctctttcg cttcggcgaa
//
LOCUS       DAOQNZ010000853         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41334_length_1917_cov_3.831901, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000853 DAOQNZ010000000
VERSION     DAOQNZ010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41334_length_1917_cov_3.831901"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PKW73_14125"
     CDS             <1..1141
                     /locus_tag="PKW73_14125"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA74479.1"
                     /translation="HVVDSLKADTQGLLFPVVMGLCLPDKEEISQSGLLDYTVNLLNE
                     FSRIWHDGLNMPFEAAVMEEGQFVLLCHGRGEDATKMLQALGPFLLSTFTKTSVVSLG
                     TGPVEGHGKTWNYGNVGVVFGVDFAVEDGGGASRRASIELLRRAARACEYAKTQGGSG
                     FLLVQASEVPQDFQDFSLGKSLSGKLGIFDVSEILQSLSSGRRTGRFTVEDRAGRLFH
                     AWLENGKLVSLHLEQDAFVLAGLDALLELISTFHGGEFTFVELPVPHQSLQELPALVN
                     CLMEAALACDQLETARLELSPGMRLKATLDGNARARLLQAEGLEEAQVAVMKEIEALA
                     REGVSLESILEQIPYSPTARKWHCALLMKRYGLLQEVGVANEASR"
     gene            complement(1122..>1917)
                     /locus_tag="PKW73_14130"
     CDS             complement(1122..>1917)
                     /locus_tag="PKW73_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HNA74480.1"
                     /translation="MPQVHGASRQAFKHAREVLSIEINSATDNPLVFAGAVISGGNFH
                     GQPLALVMDYVGMAIAELANISERRAERLVNPALSNGLPGFLTLEGGLNSGYMIAQYT
                     AAALVSENKVLAHPACVDSIPTSANQEDHVSMGTIAARKARTILDNATRVLAIELLLA
                     CQALDLRTGRVELGGREAAAQDNDKPPVDHSPREWDAKDFVPGHLLKTRLKAGVGVEA
                     AHHAVRRTVPFLVKDRQVSSDITLVEKMLRDGSLIKAVEAVTGSLH"
BASE COUNT          394 a          449 c          594 g          480 t
ORIGIN      
        1 gcatgtagtt gattcgctta aggctgatac gcaggggctt ttgtttcccg tagtaatggg
       61 gctctgtttg cctgacaagg aagaaatcag tcagtctgga ttgctcgatt acacggtcaa
      121 ccttctcaat gaattttcgc gtatctggca cgatgggctt aatatgccgt ttgaggcggc
      181 ggtcatggag gaggggcagt ttgtgcttct ctgtcatggc cgtggagaag atgcgactaa
      241 gatgttgcag gctcttggtc cctttctttt gagcaccttt accaagacca gtgtcgtcag
      301 cctggggacc gggccggtcg aaggtcatgg aaagacctgg aattatggaa acgttggtgt
      361 cgtatttggt gttgattttg ccgtcgaaga tggcggcggc gccagtcgtc gtgcctcgat
      421 tgaactacta aggagagcgg cccgagcctg tgagtatgct aagacgcaag ggggctcagg
      481 ttttctgttg gtgcaggcta gtgaggtgcc tcaggatttt caggacttca gtcttggtaa
      541 gagcctcagt ggaaaacttg gcatcttcga cgtatcggag attttgcagt ctttgagcag
      601 cgggcgtcgc accggcaggt tcacggtgga agatagggcc ggtcggctgt ttcatgcctg
      661 gctggagaat ggcaagttgg tgtctctcca ccttgaacaa gatgcttttg tcttggccgg
      721 gcttgatgcc ttgctggagt taatttccac tttccatggc ggtgagttta cctttgttga
      781 gttgcctgtg ccgcatcagt cgcttcagga acttccagcg cttgtgaatt gtttgatgga
      841 agcagcgctt gcttgtgatc aattggagac tgcccgtctg gaactctcac cgggtatgcg
      901 tctgaaagca actctcgatg gaaatgcccg tgcgcgcctt ttgcaggcgg aaggcttgga
      961 ggaagcgcaa gtggctgtta tgaaggagat agaggcgcta gcccgggagg gggttagcct
     1021 cgagagcatt cttgagcaga ttccttacag tcccacagcc agaaagtggc attgtgcatt
     1081 gctaatgaag cgctatggtc tcttacaaga ggttggtgtg gctaatgaag cgagccggtg
     1141 acagcctcca cggctttaat gaggctgccg tctctaagca ttttttcaac aagtgtaata
     1201 tcgctggaca cctgacggtc tttcacgagg aaaggcactg tgcggcgaac ggcatggtgg
     1261 gcggcctcta ctccaacacc ggctttcagg cgagtcttga gaaggtgtcc gggcacgaaa
     1321 tcctttgcat cccattccct ggggctgtgg tccacaggag gtttgtcatt gtcttgagcg
     1381 gcggcttctc ggccgcccaa ttccactctt cccgtgcgca aatcaagtgc ctgacaggca
     1441 agcagcagtt caatagcgag aacacgggtg gcattgtcga gaatagtgcg agcttttcta
     1501 gcggcgatgg tgcccatgga gacatgatct tcctggtttg cggaagtagg aatggaatcc
     1561 acgcaggccg gatgtgccag cactttgttc tctgaaacca gagccgcagc agtgtattgg
     1621 gcaatcatgt aaccggaatt gagaccgcct tccagggtga gaaaacccgg aagcccattg
     1681 gaaagcgccg gattgaccaa cctttctgct cgtctttctg aaatattggc cagttcggcg
     1741 atggccatac ccacatagtc catgaccagg gccaggggct gtccgtggaa gttgccgccg
     1801 gagatgacgg caccggcgaa gaccagggga ttgtcggtgg cggagttgat ttcaatggaa
     1861 agcacttcgc gggcatgctt gaaggcctga cggctggccc cgtgcacctg gggcatg
//
LOCUS       DAOQNZ010000854         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41339_length_1917_cov_3.259398, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000854 DAOQNZ010000000
VERSION     DAOQNZ010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41339_length_1917_cov_3.259398"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="PKW73_14135"
     CDS             <1..1375
                     /locus_tag="PKW73_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74481.1"
                     /translation="EDLRHFLLIRLRRDGTFKDFSSKIVSDGLGDKFGKSVGDYTFIL
                     DRYFNESGDESSETHEIEDKKVRELVRGEDLSNWLWSFSDTSEAGLSEARSQYSSKKS
                     LPWLLALATKIKGADKQAPELIKALAAVPQRSSGYIHARYHLARLNLERGAANARPAR
                     AIIDGVINSDIPPSARAKFATLKLETAADLSDFLSLSYVAPAAVVSGWNTIEMPENAP
                     EVEKGLKYRYEKPLLLPLSADFINYRLNLSDFLKAAAGPTIPAGRKFDFAQAAFTRAF
                     LLNDMAQLLKAQSLMKASQSAFARSLDIGAAASPEEKRFSAAFFLLQNPGARPYVTGG
                     IPRATEFNHIDDYSDNWWGTPAVSSEGLKDCKVSLPASKGVDAELKTMQKTGAAPTFL
                     SQVVLDFARNHPSDARVPEALHMVVKATRFGSGDDNTSKASAAAFKLLHSKYKGNPWT
                     KKTPYHY"
     gene            complement(1377..>1917)
                     /gene="mltG"
                     /locus_tag="PKW73_14140"
     CDS             complement(1377..>1917)
                     /gene="mltG"
                     /locus_tag="PKW73_14140"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNA74482.1"
                     /translation="ETTARALVEEMTVRFRQILKKVVGRTGNPGRLSVHEAVTCASII
                     ETEAKLQEERPVVASVIYNRLKLHMPLAMDSTVVYASKLAGKWKGNGIIYQSDLDLPS
                     PYNSRKYKGLPPGPVGCPGESSLAAALNPVRSNFLYYVRDPARADGAHTFYNNAADFE
                     VGVAKLRRWESEQRSKGLR"
BASE COUNT          414 a          537 c          509 g          457 t
ORIGIN      
        1 agaggatctg cgccatttcc tcttgattcg tctgaggcgg gacggtacat tcaaagattt
       61 ttcgagcaaa attgtcagtg acgggctggg agacaagttc ggcaagtctg tgggtgacta
      121 cacattcatt ctggatcgct atttcaatga aagcggcgat gagtcatcgg agacccatga
      181 aattgaggat aagaaagttc gcgagctggt gcgcggtgaa gacctctcca actggctctg
      241 gagcttctcc gatacttccg aggccggtct cagcgaggcc cgcagccagt atagcagcaa
      301 gaagagtctg ccctggcttc tggctctggc aaccaagatc aaaggcgccg ataaacaagc
      361 gccggaactt atcaaggcac tggcggcggt gccgcagcgc tcaagtggct acatacatgc
      421 ccgctatcat ctggccaggc ttaatctgga acgaggggct gctaacgcca gacccgcccg
      481 tgccatcata gacggtgtca tcaatagcga tattccacct tcagccaggg ccaagttcgc
      541 cactctcaaa cttgagacag ccgccgacct gagcgacttc ctcagtctct cttatgtggc
      601 tccggccgct gtggtatcag gctggaatac gatagagatg ccggaaaatg ccccggaggt
      661 ggaaaagggt ctcaagtatc gctatgagaa accgttgctt ttgccgctca gtgctgattt
      721 catcaattac aggctgaatc tgagcgactt tctgaaagca gctgccgggc cgactatacc
      781 tgccggtcgt aagttcgatt ttgctcaggc cgctttcacg cgcgcctttc tacttaatga
      841 tatggctcag ctgctcaagg cccagagcct catgaaagcc agccagagcg cttttgcccg
      901 ttctcttgat atcggggcgg cagcctcgcc tgaggagaaa cgcttttcag cggccttctt
      961 ccttttgcaa aatccaggcg ccagacctta tgtgaccggc ggcatccctc gcgccaccga
     1021 gttcaatcat atcgatgatt acagcgataa ctggtggggc acgcctgccg tttcctctga
     1081 gggcttaaag gactgcaagg tttctcttcc ggcatcgaag ggcgttgatg ctgagctgaa
     1141 gaccatgcag aagaccggtg cggctcccac ttttctctcc caagttgtgc ttgattttgc
     1201 tcgcaatcat ccttccgatg ccagggttcc cgaggctctg catatggtgg tcaaagccac
     1261 tcgcttcggc tccggtgacg acaatacatc caaggcttcg gcggcggctt tcaagcttct
     1321 gcacagcaaa tacaaaggca acccctggac caagaagaca ccctatcact attaggtcaa
     1381 cgaagaccct tgctgcgttg ctctgattcc caacgtctca atttggccac gcctacttcg
     1441 aagtctgcgg cattgttgta gaaggtgtgc gcaccatctg cccgggccgg gtctcgtaca
     1501 tagtaaagaa aattgctgcg caccgggttc aaagcggcag ccagactgct ttctcccggg
     1561 cagcccaccg gtccgggcgg cagacctttg tactttcttg agttatatgg ggagggtaga
     1621 tccagatcgc tctggtaaat gataccgttg cctttccact tgccggccag ctttgaagca
     1681 tagaccacgg tagagtccat agccagaggc atgtgcagct tgaggcggtt gtatattacc
     1741 gaagccacca ccggtctttc ttcttgcagt ttggcctcgg tctctatgat ggaagcgcag
     1801 gtgaccgctt catgcaccga taggcgcccg ggattaccgg ttcgtcctac cactttcttg
     1861 agaatttgcc ggaatctcac cgtcatttcc tctaccagag ctctggctgt agtttcg
//
LOCUS       DAOQNZ010000855         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41375_length_1916_cov_3.788286, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000855 DAOQNZ010000000
VERSION     DAOQNZ010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41375_length_1916_cov_3.788286"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKW73_14145"
     CDS             <1..990
                     /locus_tag="PKW73_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNA74483.1"
                     /translation="LFEIERLFMMYLPRICNHCANPSCVASCPSGALYKRGEDGIVLI
                     DQEVCKGWRACVAACPYKKIYYNWKTGKSEKCILCYPKLETGQIPNCFHSCVGRIRYL
                     GVLLYDADRVAEAMQVPDHALVESQRSVILDPFDKAVIEAARASGISEEFLYAAQNSP
                     VYKYVMDWKVALPLHIEYRTMPMLFYVPPLLPVMGKLENGIYEHKSEAFFSTLEKSRL
                     PIKFLAKLFAAGNESHVEAAVRKLMAVRYYRRSVDMDDIDVVEISKVMREGKTTPKEA
                     DEIYALTAIPTIDQRFVLPPIQREEVIGGGHCSPEACKGCTGLGTPVAIERGP"
     gene            992..1543
                     /locus_tag="PKW73_14150"
     CDS             992..1543
                     /locus_tag="PKW73_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNA74484.1"
                     /translation="MFDVKTSQTKNDGQSKALYELAILFQYPDQDFARKLEETSLLIG
                     SAAMAKAKESLSQYSLSQLEEIYTRTFDVAPLAAPYLSAYIYGDENFDRGALLSKLAT
                     RFQEVGFDFGQELPDHLTCLLKSASYLEPIEFEELVEFVFTPALKEMYDRLKDCESPY
                     APLMEVVRQVLAAKALPLSVSRP"
     gene            1565..>1916
                     /locus_tag="PKW73_14155"
     CDS             1565..>1916
                     /locus_tag="PKW73_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNA74485.1"
                     /translation="MPALDNFFLIALPYLAVVICLVGSFYRIKTRPMTYSALSSQFLE
                     SKGLMWGSLPWHIGIITIIAAHFIAFLVPGFWQALLSNQTVLLFVEAAGLGLSLLCLF
                     GLAALLLRRFTSEKV"
BASE COUNT          435 a          565 c          469 g          447 t
ORIGIN      
        1 ctctttgaaa ttgaacggct cttcatgatg tacctgccgc gcatctgcaa ccactgcgcc
       61 aatcccagtt gcgttgcctc ctgtccttcc ggcgcgctct acaagcgggg agaagacggc
      121 atcgttctca tcgaccagga agtttgcaaa ggttggcgcg cctgcgtggc cgcctgccct
      181 tacaagaaaa tctattacaa ctggaagacc ggcaaatcgg aaaaatgcat cctctgctat
      241 cccaaattgg aaaccggtca aatacccaat tgcttccatt cttgcgtcgg tcgtatccgc
      301 tacctgggcg ttttgctcta cgacgccgac cgcgtcgccg aagccatgca agtgcccgac
      361 catgccctgg tagaatcgca gcgctcggtc atccttgatc ccttcgacaa agccgtgatt
      421 gaagccgcaa gagcaagcgg catcagcgaa gaatttctct acgccgcgca gaactcgccg
      481 gtctacaaat acgtcatgga ctggaaggtt gccctgcctc tgcacatcga ataccgcacc
      541 atgcccatgc tcttctacgt accgccgctt ctaccggtca tgggcaaact ggaaaacggc
      601 atttacgagc ataagagtga agccttcttc agcaccctgg aaaaatccag actgccaatc
      661 aaatttctgg ccaaactctt tgcggccggc aatgagagcc acgtggaggc tgcggttcgc
      721 aagctaatgg cggtgcgcta ctaccgccgt tcggtggata tggacgatat tgacgtagtg
      781 gaaatttcca aggtaatgag ggaaggcaaa actacaccca aagaagccga tgaaatttat
      841 gccctgacgg ccattcccac catcgaccag cgttttgttc tgcctcccat tcagagagaa
      901 gaagtgatag gcggcggtca ttgcagcccg gaagcctgca agggctgcac cggtcttggc
      961 acccccgtag ccatcgagag agggccctag gatgtttgac gtaaagacaa gccaaacaaa
     1021 aaatgacggt cagagcaaag cactttacga actggctatt ctcttccagt atccagatca
     1081 agactttgct cgaaaactag aggaaaccag ccttttgatc ggcagcgccg ccatggcaaa
     1141 agcaaaggaa agcctcagtc agtattctct ttcccagttg gaagaaatct atacgcgcac
     1201 tttcgacgtg gcgcccctgg ccgcacccta cttgagcgct tatatttacg gcgacgaaaa
     1261 cttcgaccgc ggtgctctcc tttcaaagct ggctacccgc ttccaggaag tgggtttcga
     1321 ctttggtcag gagctgcccg atcacctcac ctgcttactg aagtcggctt cctatctgga
     1381 gccaatcgag ttcgaagaac tggtggaatt cgtttttaca ccggctctga aagaaatgta
     1441 tgaccgcctc aaagattgcg aaagtccgta tgcaccactg atggaggtag tcagacaagt
     1501 tctggctgcc aaagctcttc ccctttctgt ttcccgccct tagttctgcc tgggagccac
     1561 cgccatgcct gccctagata acttcttcct gatagcgctg ccttacctgg ccgtggttat
     1621 ttgtctggtc ggttccttct accgcatcaa gacccgcccc atgacctact cggcgctctc
     1681 ctcacagttt ctggaaagca aaggtctgat gtggggcagc ctgccctggc atatcggcat
     1741 catcaccatc attgccgccc actttatagc cttcctggtt cccggcttct ggcaagcgct
     1801 cttgagcaat cagaccgtct tgctctttgt ggaagcggcc gggctcggac tgtccttact
     1861 ctgcctgttc ggcttggccg ctcttttgct gcggcgcttc acttcagaga aagtac
//
LOCUS       DAOQNZ010000856         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41377_length_1916_cov_3.681891, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000856 DAOQNZ010000000
VERSION     DAOQNZ010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41377_length_1916_cov_3.681891"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1916)
                     /locus_tag="PKW73_14160"
     CDS             complement(<1..>1916)
                     /locus_tag="PKW73_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74486.1"
                     /translation="EMTEAQRPVFDEGIQLAQTVLTDGATAKDVLNFLEFGAGRGQEV
                     RAQLGKVAQDVGEQGHTQVQKLIETALNGTPETVAAAKETLRLAEEATKPGVDPLGPE
                     YKSLVEHAWRQATEFDGTQGLVPNAEGAVVDVGSHLSVAQKSLQLVRELTGETPATKL
                     VEEVSAAAGHLEKPVANQKPLDWGDTPAPARDLLRSILQLRPRNAEEHMILREGIKEW
                     ADAFPDHHSVLHQYAQITRYGDVTAMIDARLGTDYFSRFRSNQAAEPVADAQPQTART
                     TEAGDTPSVVHEPGKVTVYPERLIQDFETATGTAKQARAHLLSDHIGSMSDAQFLNWL
                     KYLHSPVDKPGAPPELTNLAAMGMSRSGVLGRPEVKALVTNPETSPLDVSTLRRFLAA
                     PEKAQTNGRLPENVTDHIAYRLETALERQKAANEMLPPEQQKAIDYGQVVTDALPSWY
                     ARQLRDTYSTRNAETGLYEYPHDFDTNLMHWLELSRQVEMANPRYRESTPQRNSVADR
                     MSVLEQAMTVRTPQNAKLVDRMLELGGQDQIAVKNMLSKLDPTTNAPEHAEMLSILAP
                     RVENMTDLKTLMDAVNFGKKADQDAFKARKDGRDTSQADKMREANESLAQSVVARILT
                     PGTPEYNRVHQIVS"
BASE COUNT          347 a          519 c          541 g          509 t
ORIGIN      
        1 cagaaactat ttgatgtact cggttgtact cgggcgtacc gggcgtaaga attcttgcca
       61 ccacagactg tgccagagac tcattggctt ctcgcatttt gtcggcctga ctggtatcgc
      121 gtccgtcttt gcgggccttg aaggcgtcct ggtcggcttt cttgccgaag ttgacggcgt
      181 ccatgagggt cttcaagtcg gtcatgtttt ctacgcgagg tgccagaata gagagcatct
      241 ccgcatgctc aggagcattt gtggtgggat cgagtttgct taacatgttt ttgacggcta
      301 tttgatcctg tccgcccaat tccagcattc tgtctaccag cttggcattt tgcggcgtac
      361 gcacagtcat agcttgctca agcaccgaca ttctgtcagc cactgaattg cgctgagggg
      421 ttgattcgcg gtaacgagga ttggccatct ctacctggcg agacaactcc agccagtgca
      481 tcagattggt gtcgaagtcg tgagggtatt cgtatagtcc ggtttccgca ttgcgggtgg
      541 aataagtatc gcgcaactgc ctggcatacc aggaaggcag cgcgtctgtc actacctggc
      601 cgtagtcgat tgccttttgc tgttcgggcg gtagcatctc atttgcagct ttttgtcttt
      661 ctaaggctgt ttccaatctg taggcaatat ggtcggtgac attttccggc aatcgcccgt
      721 tcgtttgcgc tttttctggt gcagccagga accggcgtaa agttgagacg tccagtggcg
      781 aagtctctgg gtttgtcacc agcgctttca cttccgggcg accaagcacg ccgctgcgac
      841 tcatgcccat ggcggccagg ttcgtcaatt caggcggagc gcccggcttg tccactggtg
      901 aatgcagata cttgagccaa ttgaggaact gagcgtcgga catggaaccg atatggtcgc
      961 tcaaaaggtg agctctcgcc tgttttgccg tgccggtagc cgtttcgaag tcttgaatga
     1021 gacgctcggg ataaaccgtt accttgcctg gttcatgcac caccgatggg gtgtcaccgg
     1081 cttcggttgt tcttgccgtc tgcggctgag catctgctac cggttcagcc gcttgattgg
     1141 agcggaagcg gctgaaataa tcggtgccca ggcgggcgtc tatcatagcc gtcacgtcac
     1201 cgtatctggt gatttgcgca tactggtgca aaaccgaatg atgatcaggg aaagcatcgg
     1261 cccactcttt gatgccttcg cgcaagatca tatgttcttc ggcgtttctg ggtcgaagct
     1321 gcaaaatgga gcgcagcaaa tctctggcag gggccggcgt gtcgccccag tctaaaggtt
     1381 tctgattggc cactggtttt tccaggtggc cggctgcggc tgaaacttct tcaaccagct
     1441 tagtggccgg ggtttcgcct gtcagctctc gcactaattg cagcgacttc tgagccacgg
     1501 aaagatgtga accaacgtcc actacagcac cctcggcgtt gggcaccaac ccttgtgtgc
     1561 cgtcgaactc tgtggcttga cgccaggcat gctccaccag gcttttgtat tcgggaccga
     1621 ggggatctac tcccggtttg gttgcttcct ctgccaggcg cagggtctct ttagctgcgg
     1681 cgacggtttc cggtgtgccg ttcagggccg tttcaatcaa tttttgtact tgcgtgtggc
     1741 cttgttcacc aacgtcctga gctaccttcc caagttgggc ccgcacttcc tgaccgcgac
     1801 cggcaccgaa ttccaggaag ttgagtacgt ctttggccgt agctccgtcc gtcaataccg
     1861 tctgcgccag ttgaatgccc tcatcgaaaa ccggcctttg ggcttcggtc atttca
//
LOCUS       DAOQNZ010000857         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41422_length_1915_cov_3.797312, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000857 DAOQNZ010000000
VERSION     DAOQNZ010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41422_length_1915_cov_3.797312"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PKW73_14165"
     CDS             <1..937
                     /locus_tag="PKW73_14165"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNA74487.1"
                     /translation="IAKGMVETALREGCDFIAHGATGKGNDQVRFELAVKSLAPQLGV
                     IAPWREWQYQSRTDLFDYAEAHGIPLPVTKDKPYSMDANLMHISYEGGILEDPWQEAP
                     EDIYLWTKNPEQAPDKPQFVEIEFEAGVPVAIDGEKLSAVALLEKANEIAAAHGVGRI
                     DLVENRYIGIKSRGVYETPGVTILMQAHQAVESLTLDKEVAHLKQSLVGKIAELTYNG
                     YWFAPEAQLLHNFIKDSQSTVSGTAKIKLYKGSSMVVARKSDYSLYSEKMTSFENMSS
                     FNPADSGGFINISGLRLSTWSQKHASIKKSLHPTA"
     gene            900..>1915
                     /gene="argH"
                     /locus_tag="PKW73_14170"
     CDS             900..>1915
                     /gene="argH"
                     /locus_tag="PKW73_14170"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNA74488.1"
                     /translation="MQVLRKAFTQQLDEIVTAFVNSVDADQSLIEVDIKGSIAHATML
                     TKQGLLTEPQGENIVAGLNQILQEYEDGKFTLDACFEDVHMNVEKRLQALIGEDALRL
                     HTARSRNDQVALDLRLFVLQQVERHKEQIKVLQGALAKCALANLDAVMPGYTHLQRAQ
                     PILFAHALHAFMEMFKRDGERFADLGKRASVSPLGAGAQAGSGLNIDPHFSAELLGLN
                     SCFANSIDAVSDRDFVAEYLFAAALTSTHLSQMAETFIIWATREFGFIAFSDAVTTSS
                     SLMPQKKNPDPVEIVRGKAGVMLGELVSVLATLKGLPLGYNRDLQEDKPPAIKVAKEL
                     SNCLA"
BASE COUNT          488 a          553 c          499 g          375 t
ORIGIN      
        1 catcgccaaa ggcatggtgg aaacggccct gagagaaggt tgcgacttca tcgcccatgg
       61 tgctacgggc aagggcaatg accaggtgcg tttcgaactg gcagtcaaat cgctggcacc
      121 gcaactgggc gtcattgccc cctggcgtga atggcagtat cagagccgca ccgacctctt
      181 cgactatgca gaagcccacg gcattccctt gccggttaca aaagacaagc cctactccat
      241 ggacgccaac ctgatgcata tcagttacga aggcggaatt ctggaagacc cctggcagga
      301 agcgcccgaa gacatctatt tgtggacaaa gaatccggaa caggcccccg acaagccgca
      361 gtttgtggaa atcgaatttg aagccggtgt gccggtggcc atcgacggcg agaagctctc
      421 agccgttgct ctattggaga aagccaatga aattgccgcc gctcacgggg tcggcagaat
      481 tgatctggtg gagaatcgtt atatcggcat caagtccaga ggggtttacg agacaccagg
      541 cgtcaccatt ctcatgcagg cccatcaagc cgtcgaatca ctcaccttag acaaagaagt
      601 agcccacttg aaacaaagtc tggtgggtaa aattgccgaa ctcacctata acggctactg
      661 gtttgctcca gaagcacagc tcttgcacaa cttcatcaaa gacagccaga gcaccgtcag
      721 cggtaccgcc aaaatcaagc tgtacaaagg tagttccatg gtggtggccc gtaagtcgga
      781 ttactcactt tatagtgaaa aaatgacgag ctttgaaaac atgtccagct tcaacccggc
      841 cgactcgggc ggtttcatca atatcagcgg acttagacta tctacctgga gccagaagca
      901 tgcaagtatt aagaaaagcc ttcacccaac agcttgatga aatcgttacc gcctttgtca
      961 actccgtcga cgccgaccag tccttaatcg aagtggacat caagggcagt atcgcccacg
     1021 ccaccatgct caccaaacag gggctcctga ccgagcccca gggtgaaaac atcgtggccg
     1081 ggctaaatca aatcttgcaa gaatacgagg acggcaagtt cacactggat gcttgcttcg
     1141 aagacgtgca catgaatgtg gagaaacgac tgcaagccct aattggagag gacgcgctcc
     1201 gcttgcatac ggccagaagc agaaacgacc aggtagcact ggatctgcgt ctgtttgtgc
     1261 tgcagcaggt agagaggcac aaagagcaga tcaaggtctt gcagggcgct ctagccaagt
     1321 gcgccctggc taatttagac gccgtcatgc ccggctacac acatctgcaa agggcccagc
     1381 cgatactctt tgcccacgct ctgcatgcct tcatggaaat gttcaagcgc gatggcgagc
     1441 gcttcgccga cctcggcaaa agggcctcgg tctcgcccct gggtgcaggc gcccaggccg
     1501 gctctggtct caatatcgac ccccatttca gcgccgaact cctgggtctg aatagctgct
     1561 tcgccaattc catcgatgcc gtttccgacc gcgatttcgt agccgaatat cttttcgcag
     1621 cggcgctcac ttccacccat ttgagccaga tggccgaaac cttcatcatc tgggccacca
     1681 gagagttcgg cttcattgcc ttcagcgacg ccgtcaccac ttcttccagc ctcatgcccc
     1741 agaagaagaa cccggacccg gtagaaattg tcagaggcaa agccggcgta atgttaggtg
     1801 aactggtaag cgtactggcc accctcaaag gtctgcccct cgggtacaac cgcgacttgc
     1861 aggaagacaa gccgccggcc atcaaggtag caaaggaact gagcaactgc ctggc
//
LOCUS       DAOQNZ010000858         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41463_length_1914_cov_3.839161, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000858 DAOQNZ010000000
VERSION     DAOQNZ010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41463_length_1914_cov_3.839161"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKW73_14175"
     CDS             <1..429
                     /locus_tag="PKW73_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74489.1"
                     /translation="PRLSGQVWNPTDESINALGMKVEIVNLDGQVLWSKETRVVDEYV
                     PPLGAREGKAVDITGGESVKADGKSQFKVYFDGKLYNSYPIGLKEKKVVKEVEKEPAA
                     ETTTENNSAQPLPTPMPGTLERPEPPAKNSAEEKTMKDLE"
     gene            complement(440..1111)
                     /locus_tag="PKW73_14180"
     CDS             complement(440..1111)
                     /locus_tag="PKW73_14180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNA74490.1"
                     /translation="MTEKKYDVDEGWAFAKENKFSKMVNETRFDRAKFKATKNRETAE
                     RERKPLTEAEMAEFEKASTIDALTGVLNFKTFYRKLDYELRRAKRYKRPLSLMLVQLD
                     NLDNCKRQYGAMVEDEMVKSAVRLILGCIRDVDVCGRAEGSDIAVIFPETYSSKAMVV
                     AERVRDKLRSESINNDLRHLRVTASIGVVSFPTHARDEHDLLQKALQFAGHAREEGGD
                     MVFNG"
     gene            complement(1198..>1914)
                     /locus_tag="PKW73_14185"
     CDS             complement(1198..>1914)
                     /locus_tag="PKW73_14185"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNA74491.1"
                     /translation="ANTVLTLVGDFDVDKACALLQETFSSGQWPGDGKGPSPIKVEAD
                     VLEKVSDKTPLVSKLAEKANVDIVLARPVNLSVRSSDYLAALVANAVLGYDSFACRLA
                     PVRDRYGLTYGIYSRIVDPEFPYGPWSIELSVNPENVKRALEIVKKIVGEFDKGGISP
                     GELAKEKSHLAGAYLVGLRSPRALARKICEYEQLGLPLKNLDNFRWRLSKITLKDVNE
                     AIRKHFDVTTMQVSLSGTLD"
BASE COUNT          455 a          513 c          505 g          441 t
ORIGIN      
        1 cccagattat cgggtcaggt gtggaatccc accgacgagt caatcaatgc tcttggtatg
       61 aaagttgaaa tagtcaatct ggacggtcag gtgctctgga gcaaggaaac ccgcgtggta
      121 gacgaatatg tgccgcccct cggtgccaga gaagggaaag cagttgatat taccggtggt
      181 gagtcggtga aagccgacgg caagtcgcag ttcaaagttt attttgacgg caagctctac
      241 aacagctatc ccatcgggct gaaagagaaa aaagtagtca aagaagtcga gaaagaacca
      301 gcggcggaga ccaccaccga aaacaacagc gctcagccct tgcccactcc catgcccgga
      361 acactggaga gaccggagcc tcccgccaag aattcggcgg aagaaaaaac catgaaggac
      421 ctggagtaat ttcgacgggc taaccgttga agaccatgtc gccgccttct tctctggcat
      481 ggccggcaaa ttgaagggct ttctgcaata aatcgtgctc gtctcgagca tgggtgggga
      541 aggaaaccac accgatactg gctgtcaccc tcagatgtct gagatcgtta ttgatgctct
      601 ccgagcgcag tttgtcccgt actctttctg ctaccaccat ggctttggaa gaataggtct
      661 cgggaaaaat aacggcgatg tccgagcctt ccgcccttcc gcaaacatcc acatcgcgaa
      721 tacagcccag gataaggcgc accgccgact taaccatctc atcttccacc atggcaccgt
      781 actgacgctt gcaattatca agattgtcca attgcacgag catgagagag agcggtcgct
      841 tataccgctt ggcccggcgc agttcataat cgagcttgcg gtaaaaagtt ttgaaattga
      901 gcacgccggt gagcgcatca attgtggaag ccttctcaaa ctcagccatt tcggcttcag
      961 tgaggggctt gcgctccctt tcggcggtct cgcgattctt ggttgccttg aatttggccc
     1021 tgtcgaagcg agtctcattc accatcttgg agaacttatt ctccttggcg aatgcccatc
     1081 cttcgtctac gtcgtacttc ttctcagtca tggttcaaac cggtctctag tccttgttgt
     1141 ctactttgac ttacccgagc cctaaagttt ctaatctata agactggccg tctccgtcta
     1201 atctagagtg ccggagagtg aaacctgcat ggtagtcaca tcaaagtgct tacggatggc
     1261 ttcattgaca tccttaaggg taattttgga aagtctccag cggaagttgt ccagattctt
     1321 gaggggcagt ccgagctgct catactcgca aattttccgg gccagggccc gggggctgcg
     1381 caaaccaacc aggtaggctc cggccaggtg ggacttttcc ttagccagtt ccccggggct
     1441 aatgccgcct ttatcgaatt cgccgacaat ctttttgact atttcgagtg cccgtttgac
     1501 gttctcagga ttgacagaaa gctcaataga ccagggtcca taaggaaact cggggtcgac
     1561 gatacgggaa tagatgccgt aggtgagacc gtagcgatct ctgaccgggg ccaggcgaca
     1621 ggcaaaactg tcgtagccga gcacggcgtt ggctaccagg gcagccagat agtcgctgga
     1681 gcgcacgctg aggttgaccg gtcgggcaag cactatgtcc acattggctt tctcggccag
     1741 tttactgacc agaggagtct tatcggacac cttctcgagc acatccgcct ctaccttgat
     1801 gggtgaaggt cccttgccgt caccgggcca ttgcccgctg gaaaaagtct cttgcagaag
     1861 ggcgcaggcc ttatctacat cgaaatcgcc caccagtgtc aatacggtat tggc
//
LOCUS       DAOQNZ010000859         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41560_length_1912_cov_2.998384, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000859 DAOQNZ010000000
VERSION     DAOQNZ010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41560_length_1912_cov_2.998384"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKW73_14190"
     CDS             <1..612
                     /locus_tag="PKW73_14190"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74492.1"
                     /translation="VPAQSSGPAILPAREDAIGGGNPFEGLGRADDPNPSRVEKYQTQ
                     TPAAEAETAPAASPESSTKTSEAEAAAREKPAVAGEKPSQSGDKPPQAGDKPTPPGEK
                     KKEQRKWKLLSEPKTLSAVPDPMVKEALREMQKGRYQDALKHLEGLKRLNPNAPELDY
                     LMAVACVMSLRFEEAREHYEKVLQSQAPPELHDLARKGLNKLK"
     gene            complement(656..1408)
                     /locus_tag="PKW73_14195"
     CDS             complement(656..1408)
                     /locus_tag="PKW73_14195"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNA74493.1"
                     /translation="MVDAKTVKVFAHRGGREWAPENTMAAFRQALDLRVDGIELDVQR
                     CSTGELVVFHDEDLSRTTNGVGLVADVSFDELRRLDAGSWFDKEFKGEKVPTLEEVLN
                     LVDGKCILNIEVKNAPVSYEGIEDDLLALIESYPHPETLILSSFDHYVLRNLAQKAPQ
                     YQLAVLADAVLIDLPAYAATFGARYFHPCFDSCRPDVVEQCHQAGIGVNPWTVNGRKA
                     WSRALKMQVDGIVTDDPEDLMVFLGRAVAVTV"
     gene            complement(1550..>1912)
                     /locus_tag="PKW73_14200"
     CDS             complement(1550..>1912)
                     /locus_tag="PKW73_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74494.1"
                     /translation="GYLVHRVLMETFGSVDEIPGLIRAIASHVREVIRQSNVISIVNE
                     HPTTERWGSYIIKQKERMRFEVGMEKDSLVLKNIVGLVGMEHGVEAPLEKITVKPPKL
                     IVTLNMGILHPQKVLDLK"
BASE COUNT          496 a          571 c          488 g          357 t
ORIGIN      
        1 gtgccagccc agagctccgg accggccatt ctaccggccc gggaagatgc cataggcggc
       61 ggcaacccct ttgaagggct gggccgagcc gatgatccaa acccctcccg ggtggaaaaa
      121 tatcagaccc agactcccgc cgccgaagcc gaaaccgctc cagcggcaag cccggaaagc
      181 agcaccaaaa cttctgaagc agaagccgcc gccagagaaa agccggccgt ggcgggggaa
      241 aaaccgagcc agtcaggaga caagccgccc caggcagggg acaagccgac tccgccaggg
      301 gaaaagaaaa aggaacagcg caagtggaaa ctgctttccg aacccaagac tttatcggca
      361 gtgcccgacc ccatggtcaa ggaagccctg cgtgaaatgc agaagggccg ctaccaggat
      421 gctctcaagc acctggaggg tctcaaacgc cttaacccca atgcaccgga attggactac
      481 ctgatggccg tcgcctgcgt gatgagcttg cgttttgaag aagcccgaga gcattacgaa
      541 aaggtcttgc agagtcaggc gccgcccgaa ttgcatgacc tggcccgcaa aggtctcaat
      601 aagctcaagt gaaacacagg catcaagcca atgaactgac cgccgcaaac tctcgtcaga
      661 cggtaactgc cacagcccgc cccagaaaga ccatcaggtc ttcgggatca tcagtgacaa
      721 tgccgtccac ttgcatcttc aaagcgcggc tccaggcctt gcgcccgttc accgtccagg
      781 gattgacgcc tattccggcc tggtgacatt gctccaccac atcggggcga cagctatcaa
      841 agcagggatg gaaatacctg gctccaaagg tggccgcata ggccggcaga tcaattagaa
      901 cggcatcggc caggaccgcc agttgatact ggggagcttt ctgggccagg ttcctcaaca
      961 cataatggtc aaaggagcta agaataaggg tttccggatg gggataggat tcgatgagag
     1021 ccaggagatc gtcctcgatg ccctcatagc tcaccggtgc attcttaact tcaatgttga
     1081 gaatacattt gccgtccacc aggttcagca cttcctccag agtgggcacc ttctcgccct
     1141 tgaactcctt gtcgaaccag gaaccggcat ccagtctgcg caattcgtca aaactgacat
     1201 cagccaccag cccgacgccg ttggtggtgc ggctcaggtc ttcgtcgtga aaaaccacaa
     1261 gttcgccggt ggaacagcgc tgcacatcaa gctcgatgcc gtccacccgt agatcaaggg
     1321 cctggcgaaa agcggccata gtgttctcag gcgcccactc acgaccgcct cgatgagcga
     1381 agacctttac ggttttggca tccaccactg catatcctct ttagttgaag cacctgaact
     1441 ctgttctgaa ccaggcccgg aaccggcccc aatccagaaa taatttcaga aaatacttcc
     1501 gaaatcagtt cagaaaccat aagagtctag cagacctgaa tgcccctgct tacttgagat
     1561 cgagcacttt ttgcggatgc aaaatgccca tattgagagt aacaatgagc ttgggaggct
     1621 tgacggtaat cttctccagg ggagcctcta caccgtgctc catgcctacg agaccgacaa
     1681 tattcttcaa taccagagaa tctttctcca tgcccacttc aaagcgcatg cgctctttct
     1741 gcttaataat gtaagagccc cagcgttcgg tggtgggatg ctcgttcaca atactgatga
     1801 catttgactg ccgaattacc tcacgcacat gggaagcaat ggctctgatc aggccgggaa
     1861 tctcatccac cgagccaaaa gtctccatca aaacccgatg caccaggtaa cc
//
LOCUS       DAOQNZ010000860         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41678_length_1909_cov_2.968716, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000860 DAOQNZ010000000
VERSION     DAOQNZ010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41678_length_1909_cov_2.968716"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PKW73_14205"
     CDS             <1..1422
                     /locus_tag="PKW73_14205"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNA74495.1"
                     /translation="HPSNKTAGFLVLTATASYLCLAAIGKSSMPVRPQFEYESNSAVQ
                     KLKEVTDTKGRMVASGGHLLRPNSNVVYQLKDLRSHNPLFPKRYLTFMQESGARVDVF
                     SQEFEAPLSALLGAGSVNAVASLTPVRSLSDQSRAISETTHQEAAVRLPLTAKSGLSL
                     ELGKAQLDLDNRAVYLPVRLVGGTKSTLENLAIVYVLLSDKDDTLWFSDQQAASAMAD
                     AAEKEKLPQFVGFPLPRDLKVGSQVKLALQLFDTRAGLFLPVPDGKTSKNSAGQILTV
                     VSLVKSPTALDTTAKDWQLVAETAERIRIYSNAGAFPRAYFSTNLKLVNSEEAALSSL
                     KESAEKDLPSVILEREEKEREPTPVLPAISKPVQIVAETANQIKLKVETDRAGFVVLN
                     DIYYPGWTARLDGKPVAIRKANYLMRAVACPDGVHELVFSYEPDSFKYGLLALATALL
                     SGLVLFCRRRSLAGGSPRTEQAQ"
     gene            complement(1467..1757)
                     /locus_tag="PKW73_14210"
     CDS             complement(1467..1757)
                     /locus_tag="PKW73_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74496.1"
                     /translation="MFMQGGLESSNTLSFASLPIDEVLALQNSLLAAAVENGSGYQCS
                     LEWAFFIAGLCFTAVFVVSLLYIFVVDTVSEASELDRRIQALSAHQEGARQD"
BASE COUNT          513 a          532 c          480 g          384 t
ORIGIN      
        1 caccccagca ataaaacggc cggattcctg gttctcaccg ccacagcttc ttatctctgc
       61 ctggccgcta tcggcaaaag ttcgatgcct gttcgtcccc agtttgaata cgaaagcaat
      121 agcgcggtgc aaaagctcaa ggaagtcacg gacacaaagg gcagaatggt ggcctccggc
      181 ggacacttat tgagaccaaa cagcaatgtc gtctaccagc tgaaggacct gcgcagccat
      241 aaccccctct tccccaaacg ctaccttact ttcatgcaag agagcggagc ccgggtcgac
      301 gtcttcagcc aggagttcga agcgccatta agcgccctgc tgggagcagg ttcagtaaat
      361 gctgtagcat cactgacgcc ggtgcgttcc ctgtccgatc agagccgcgc cataagcgaa
      421 acaacacacc aggaagcggc tgtcagacta ccactgacgg caaaatccgg actgtcgctg
      481 gagttgggga aagcccaact tgatctggat aaccgtgcag tttacctgcc cgtcaggctg
      541 gtaggcggaa caaaatccac gcttgagaat ctcgctatag tctacgtact acttagcgat
      601 aaggacgata ccttgtggtt ttccgatcag caagcagcca gtgcaatggc cgatgccgcc
      661 gagaaagaaa agctaccgca gtttgtgggc ttccccctcc ctcgcgacct gaaagtcggc
      721 agccaggtaa agctggccct gcagctcttc gataccagag ccgggctgtt tctgccggta
      781 ccagacggaa agacaagcaa gaatagcgcc gggcagatcc tgaccgtggt aagcctggtt
      841 aagtcgccaa cagcgctgga cactacggcg aaggactggc aattagttgc tgaaactgcg
      901 gagagaatta gaatttacag caacgccgga gcctttccca gagcctattt ttctacaaac
      961 ctcaagctcg tgaattcgga agaagcggcc cttagcagcc tgaaagagtc cgccgagaag
     1021 gatcttccat ctgtaattct ggaaagagaa gagaaagaga gggagccgac accggtatta
     1081 ccggcgatat caaagccagt ccagatagtg gccgagactg ccaatcaaat caagcttaag
     1141 gtagagaccg atagagccgg cttcgtggtt ctgaacgaca tctactatcc cggatggaca
     1201 gcccgactgg acggcaaacc agtagcaatc aggaaagcca attacctgat gcgggccgta
     1261 gcatgtccgg acggagtcca tgagctggtt ttttcttatg aaccggacag cttcaaatac
     1321 ggacttctag ctctcgctac cgctctgctt tccggtctgg tacttttctg cagacgccgc
     1381 agcctggcag gcggcagccc ccggacggag caagcgcaat aaatgcagct ctggatttcg
     1441 taaaatcagc cccagttttt agctcactaa tcctgccgcg ccccctcctg atgggcagac
     1501 agggcttgaa tgcgcctgtc aagctcactc gcttcgctga cagtatcaac tacaaagata
     1561 tagaggagac tgacaacgaa aactgccgtg aagcaaagac cggctataaa gaaggcccac
     1621 tcgagcgagc attgataccc gctcccattt tcgacggctg cagccagaag cgagttctgc
     1681 aaagccagca cttcatcgat tggcagcgaa gcgaaactca gagtattact agattccaga
     1741 ccgccttgca tgaacataag cactctccca aagccctatg tccttaataa cgtcaggcgg
     1801 agagcaaaag ttcaaaatga aagcctagat ttaagcaaac tcctctaaat tgagccctgg
     1861 caggacccaa gatatttctc catttttctt tgatacatcg cagctaaaa
//
LOCUS       DAOQNZ010000861         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41726_length_1908_cov_2.760928, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000861 DAOQNZ010000000
VERSION     DAOQNZ010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41726_length_1908_cov_2.760928"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="PKW73_14215"
     CDS             complement(<1..1269)
                     /locus_tag="PKW73_14215"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNA74497.1"
                     /translation="MQPFDALSIRAVLNEARPLIVNRKVDKISQLGRDELVITLRGKS
                     GLTALFASAQSVHGRICLLQPNSLQRKSGDRPDPASERYVSKYGANSTVPNFCLLLRK
                     HLSGATLIAAEQPLGERIVDLVFSAVDEVGTSSIKVLTAEIMGRHSNLIFWDKASSKI
                     ITASHLVTRDMSRQREVAPGLKYERPPSQDRPSLYTVSSEDFRKLFDKLKEQGKNGGA
                     EAETPGFATLEQWIIATFTGAGRHLCEELVLSAGLESEIGKALNVENAAEKLWQTVES
                     MRKDGVRNPLHKPLLFKDLSRYSVLGTFPHKPEEVKTFPSVNDLIEEYFRTCEHNEQV
                     NQLRERLKADLSLELSKLESRVKTASEHVQSAAEIDALKRSGDLILANLTSISAGQEV
                     LLTENIFVDNGGQLSIKLNPNLTVSQNAQHY"
     gene            1352..>1908
                     /gene="gmk"
                     /locus_tag="PKW73_14220"
     CDS             1352..>1908
                     /gene="gmk"
                     /locus_tag="PKW73_14220"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNA74498.1"
                     /translation="MNTSNPLTILGPLKPGETIIETDTRAKTEPRKGQKGKRPARMGN
                     LLVITGPSGVGKGTLVKRLMPRIDKLKYSVSVTTRPMRPGEVEGTSYYFCTSAEFEDM
                     IRQGAFMEHAKFAGNYYGTPRGWVSEELERGVDVILEIEVQGAKQIHSQYPDAVMIFI
                     SPPSFDELATRLKERGTESEDKINL"
BASE COUNT          400 a          508 c          539 g          461 t
ORIGIN      
        1 gtagtgctgg gcattctggc tgacagtcag gttcgggttc agtttgatgg ataactgacc
       61 gccgttgtcc acaaagatat tttccgtcag aagaacttcc tgaccggcgc ttatggaagt
      121 caaattggcc aggatcagat caccggagcg cttgagagcg tctatttccg ccgcgctttg
      181 cacgtgttcc gaggctgttt tgacgcggct ttccagcttg gacaattcca ggctgagatc
      241 ggccttcagt ctttcccgca gttgatttac ctgctcgtta tgctcgcaag tcctgaaata
      301 ttcctcgatt aagtcgttta ccgagggaaa ggtcttaact tcttccggct tatgagggaa
      361 ggtgcccagc actgaatagc gacttaagtc tttgaagagc aggggcttgt gaagcgggtt
      421 ccgcaccccg tccttgcgca tactttccac ggtctgccag agtttttctg cggcattttc
      481 cacgttcaag gccttgccga tctcgctttc cagaccggcc gatagcacca gttcttcgca
      541 gaggtgccgc ccggcaccgg tgaaggtggc aatgatccat tgctccagtg tggcaaagcc
      601 tggtgtctcg gcctcggcgc cgccgttttt gccctgctct tttagcttat cgaagagctt
      661 gcggaagtcc tcactgctga cggtatatag gctggggcgg tcctgactgg gcggtctttc
      721 gtatttgaga ccgggggcta cttcccgctg gcggctcata tcacgagtga ccagatggga
      781 ggccgtgatg attttgcttg aggctttgtc ccagaaaatc aggttgctgt ggcgtcccat
      841 aatttctgcc gtcaaaactt taatggagga tgtgcccacc tcatcgacgg cggaaaagac
      901 aagatcgaca atccgctcac cgagaggctg ttcggctgca atgagcgtgg ctcccgaaag
      961 atgtttgcgc aacaagaggc agaaattggg caccgtgctg ttggcaccat acttggagac
     1021 atagcgttcg cttgccggat cgggccggtc gccgcttttg cgttgcaggc tgttgggttg
     1081 caacaggcag atccgaccgt gcaccgattg ggccgaggca aagagtgccg tgagaccgct
     1141 tttaccgcgc agagtgatga ccagctcgtc ccggcccagt tgacttatct tgtctacttt
     1201 gcgattgaca attagcggtc tggcctcatt gaggacagcc cttatggaga gcgcgtcaaa
     1261 tggttgcatg acaatgcggt ttctgcggtt gcacggtgag ctcagttaag gtttaatagc
     1321 tgacaattta tcaaactcgc gtgggttttt tatgaacacc tccaacccac ttacaattct
     1381 tggtcctctc aaaccgggtg aaacaatcat agaaaccgat accagagcaa agaccgagcc
     1441 gaggaagggt cagaagggca agcgcccggc ccggatgggt aacctcttgg tcattaccgg
     1501 tccctccggg gtcggcaagg gcaccctggt gaagcggctc atgccccgca tcgacaaact
     1561 caaatattcc gtctccgtca ccacccggcc catgcgcccc ggggaggtag agggtacttc
     1621 ttactacttt tgcacgagcg ctgaattcga agacatgatc aggcagggtg ccttcatgga
     1681 gcatgccaag tttgccggca actattacgg taccccccgg ggctgggtct ccgaagaact
     1741 ggagcgaggc gtcgacgtca tccttgagat cgaggtgcag ggcgccaaac agattcattc
     1801 gcagtacccg gatgcggtca tgattttcat ttcgcccccg tctttcgatg aactggccac
     1861 ccgtctgaaa gagcgaggca ccgaaagtga ggataagatc aatctaag
//
LOCUS       DAOQNZ010000862         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41752_length_1907_cov_3.289957, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000862 DAOQNZ010000000
VERSION     DAOQNZ010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41752_length_1907_cov_3.289957"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..>1907)
                     /locus_tag="PKW73_14225"
     CDS             complement(93..>1907)
                     /locus_tag="PKW73_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74499.1"
                     /translation="RVAFHSVDKVGFYGNGSDAGKAEAGKLADVEGEKRRSFSPTENT
                     GTYLDDGVKKATSLFGQVVLGESEEASRSHVEVEKYSKMFLKAAPLFISRKVGLSMTL
                     GMFGLDQARPEDSFGTQAADFALGATKGAALKGTMQGLSALNASPAMKGVAMGMTDRT
                     VQTALTRQTWVDQNTGEGSLLAGISSISLTVADPRAIGMDALLFGVTEFTMGRLNYAT
                     AGALYRHPALPVMASGGIYGLGSGAGEEIIRQRREREDFDFSKVLYRAGMAGGVSALA
                     AGVGGLDKARSLKIQGSENRTLGDTKAPVPERVAKFNEFDSRQMELKSSPLKITEQLN
                     DVAYMAEINVAGGSKKVLFRLTETPDMKARFANELLDYQLHRALKPGTDSRAVAPVEA
                     MINGQKRQGYVQELSGRSFEDGLRDRAAQQYDLLTDRAAARVLRNDPFLMNQYEKLWA
                     ERMLMAEWDNHSHNMLMGAGGRLSNIDFAKALPDAKYVTDYVPTWGKTASRVSLLNDR
                     LVGSLSGKPLSADTRDMMTQFVRDFDTGVGRQALSAAGHSPQQVDGLLGRARWFSENG
                     TLPKQDVPNYFVQASLRALYWLKKGRSQRFEQEAETAR"
BASE COUNT          427 a          576 c          490 g          414 t
ORIGIN      
        1 ccagccatct tccaagccac agggtaggag cagtagttgc ccctggggaa accacactgc
       61 aaaacaagac ggccgccact tccgagcact gatcagcggg cagtctccgc ctcctgctca
      121 aagcgctgag aacggccttt cttaagccag tagagagccc gcaaagaagc ctgcacaaaa
      181 tagttgggca catcctgctt cggcagcgtg ccgttctcag agaaccatcg ggctcgtccg
      241 agcaaaccat ccacctgctg aggggaatga ccggcagcgg aaagtgcctg acgaccaacg
      301 cccgtgtcaa agtctctgac aaactgggtc atcatatcgc gagtatctgc tgagagaggc
      361 tttccagata aggaaccaac cagcctgtcg ttcagaagtg atacgcggct tgccgtttta
      421 ccccaggtag gcacatagtc ggtaacgtac ttggcgtcag gaagagcctt ggcaaagtct
      481 atattgctaa gtcttccccc ggctcccatc agcatattgt ggctatgatt gtcccattca
      541 gccatgagca tgcgctcggc ccagagcttt tcatactggt tcatcaaaaa cgggtcgtta
      601 cgcaacacgc gggcggccgc tctgtctgtc aaaaggtcat actgctgggc agcccgatct
      661 ctcaaaccgt cttcgaaaga acggccggaa agctcttgca catagccctg ccgcttttgc
      721 ccattgatca tggcttccac tggggctacg gcccgcgaat cagtgccggg tttgagagcc
      781 cggtgcaact gataatcgag caattcattg gcgaaacgcg ctttcatatc cggcgtttcc
      841 gtcaggcgga agagcacctt tttgcttccc cctgccacat tgatctccgc catgtaagcc
      901 acatcattca actgttccgt gatcttgagc ggtgaggatt ttagctccat ttgtcgagaa
      961 tcaaactcgt taaacttggc cactcgctcc ggcactggtg ccttggtatc acccaaagtg
     1021 cgattttccg agccttgaat cttgagagac cgagctttat ctagcccacc cacaccggcg
     1081 gccagggcac tgacaccgcc tgccattccc gcccggtaaa gcactttgga aaagtcgaag
     1141 tcttcccgct cccggcgctg ccgaataatt tcctctccgg caccgctgcc cagaccgtaa
     1201 atgccccccg atgccattac cggtagtgcc ggatgacgat agagagcacc ggcggtggca
     1261 tagttcaaac gccccatggt aaattccgtg acgccgaaca ggagagcgtc catgcctata
     1321 gcccgcggat cggcaactgt caaagaaatt gaactgatac cggccaagag agacccttcg
     1381 ccggtgtttt gatctaccca ggtctgacgg gtaagtgcgg tctgcacggt gcgatcggtc
     1441 atgcccatgg ctacgccctt catggccggg gaagcattca gggccgaaag tccctgcatg
     1501 gtacccttga gagccgcacc tttggtggca cccagggcga aatcggcagc ctgtgtacca
     1561 aaactgtctt ccggtctagc ttgatccaga ccgaacatac caagggtcat ggacagcccc
     1621 actttgcggc ttataaacag cggcgcagcc ttgagaaaca tcttgctgta tttctctact
     1681 tccacatgac tacgggaagc ctcttcgctt tcccccagga caacctgacc gaagagactg
     1741 gtggctttct tgacgccgtc atccagataa gtgccggtgt tctcagtggg cgagaaagag
     1801 cgccgctttt cgccctcaac gtctgcaagt ttccctgctt ctgctttgcc ggcatcggag
     1861 ccattgccgt aaaatccaac cttgtcgaca ctgtggaagg cgacgcg
//
LOCUS       DAOQNZ010000863         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41804_length_1906_cov_3.895732, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000863 DAOQNZ010000000
VERSION     DAOQNZ010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41804_length_1906_cov_3.895732"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /gene="lon"
                     /locus_tag="PKW73_14230"
     CDS             complement(<1..1635)
                     /gene="lon"
                     /locus_tag="PKW73_14230"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNA74500.1"
                     /translation="MVDVSTTVFPLIPLRDVVVYPQMVTPLFVSRQRSIAALEQALMR
                     DDRLVVLVAQKDPETEEPKVEDLYQVGTLAEVMQMLKLPDGTVKVLVEGSSRINVTEI
                     SEVPEHFTASVSSITEVVTDDEATRTLIKISVDKFEQYVKSTKKINPESLLAVSGIGQ
                     PGRLADIIATYLGLSLAERQKCLEIVDSTERLEYIGQLLSRELELAELEQQIHDRVRF
                     NLEKTQREYYLREKLRIIQEELSSDGYELGEINEYKQKIKKSKMAGVALEKAMKELGR
                     LEKMMPQSAEAAVIRTYLDTLVSLPWAKRSRESIDLKVADEILAEDHYGLEKVKERIL
                     EYLAVRKLAKEPTGTILCLVGPPGVGKTSLVKSIARAMNRQFARISLGGVSDESEIRG
                     HRRTYIGAMPGRILQAVKQAGTKNPVILLDEIEKMTRSYQGDPMAAMLEVLDPDQNDS
                     FTDHYLDAPFSLSEVVFVATANTLDYVPKPLYDRLEVIPLSGYTEEEKLSIAEQHIIP
                     KTLKKHGLTSKELKVPAAAVRRIIQEYTREAGVRSLE"
BASE COUNT          428 a          520 c          475 g          483 t
ORIGIN      
        1 ctccagggag cgaactccgg cttccctggt gtattcctga atgatgcggc gaactgccgc
       61 cgccggcact ttcaactcct tagaagtcaa accatgcttc tttaaagtct tgggaataat
      121 gtgctgctcg gcgatggaga gcttctcttc ttccgtatag ccggaaagcg gtataacttc
      181 caggcggtca tagagcggct ttggcacata atcgagagta ttggcggtgg ccacgaagac
      241 cacttccgaa agggaaaagg gcgcgtctaa ataatggtcg gtaaaactat cgttttgatc
      301 ggggtccaaa acttccagca tggccgccat gggatcgccc tgatagcttc tggtcatttt
      361 ctcgatttca tcgagcagga tgaccgggtt tttggtgccg gcttgcttga cggcctgcaa
      421 gatacgaccg ggcatggcgc caatataagt gcggcgatgt ccgcgaatct cagactcgtc
      481 ggatacaccc cctaagctga tacgggcaaa ctgccggttc atggcccggg caatagattt
      541 gaccaggctg gtcttaccca caccgggagg accgaccaga cagagaatgg tgccggtagg
      601 ctctttggcc agcttacgta ccgccaggta ttccagaata cgttctttca ctttctcaag
      661 accgtagtga tcttcagcca gtatttcatc ggccactttc aggtcgatgg actcgcggga
      721 acgcttggcc cagggcaggg aaaccagggt atcgaggtag gtgcgaataa cagccgcttc
      781 cgccgactgc ggcatcattt tttcaagccg ccccagctct ttcatagctt tctcaagagc
      841 gacaccggcc atcttgctct tcttgatttt ctgcttgtat tcgttgattt cgcccagttc
      901 gtaaccatcg gaagaaagct cttcctgaat gatgcgcagc ttttcgcgca aatagtactc
      961 gcgctgagtc ttctctaaat tgaaacgcac ccggtcgtga atctgctgct ccaactcggc
     1021 caattccagt tcacgactga gcaattgccc aatgtactcc agcctttcgg tggaatcgac
     1081 tatttcaaga catttctgcc tttcggccag ggaaagcccc agataagtag cgataatatc
     1141 ggccaaccgc ccgggctgcc cgatgcctga gacggccagg aggctttccg gatttatttt
     1201 cttggtggat tttacatact gctcgaactt atccaccgag attttgataa gtgtgcgggt
     1261 ggcttcgtcg tcggtaacca cttccgtgat ggaagagacc gaagcagtga aatgctccgg
     1321 tacttctgaa atctccgtca cattgatacg ggaagagccc tctaccagca ccttcacagt
     1381 gccgtcaggc agcttcagca tctgcatcac ttccgccagg gtgccgacct ggtataggtc
     1441 ttccacctta ggctcttccg tttccgggtc tttctgcgcc accagaacca ccagccggtc
     1501 atcccgcatc aaggcctgct ccagagcagc aatggaccgc tgtctggaaa caaacagcgg
     1561 tgtgaccatt tgcgggtaga ccactacgtc cctcaagggg atgagcggaa agaccgtagt
     1621 gcttacgtct accataaggc tagttaccct cgcttaacgg gtcggtaggt atgagcatcg
     1681 cccggctttt caagctaact ttctcaagcc aactttttca agctgtcttt atcaagctaa
     1741 cttcttcaag ctaacttctt caagctaagc gatctcaccc ttaagtttag cctttggaag
     1801 ctgaagtaac tcagcagtcg atcgtttttc tacaagttcc ttagtgatat ggcagacctt
     1861 gatatcggta cgggaaggaa cctcgtacat gatgtcgagc attatc
//
LOCUS       DAOQNZ010000864         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41807_length_1906_cov_3.715289, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000864 DAOQNZ010000000
VERSION     DAOQNZ010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41807_length_1906_cov_3.715289"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..>1906)
                     /locus_tag="PKW73_14235"
     CDS             complement(125..>1906)
                     /locus_tag="PKW73_14235"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74501.1"
                     /translation="SNKAPQMLPKPFADFEDIVAERIEEADGFYQALYTEDMKEDQEK
                     INLFRQAMAGMLWSKQYFYYDVERWLKEHDAGFLNRASKRSNVRNSDWYHMYNDDIIS
                     MPDKWEYPWYAAWDLAFHTVPLGTVDIDFAKDQLDMMLRNDYQHPNGQIPAYEWNFGD
                     VNPPVHAFATIEVYLHDKVRNNGKGDVDFLKYAFTKLIVNYSWWLNRKDPKGKNVFEG
                     GFLGLDNIGVFDRSAPLPTGGSLEQADGTAWMAFFSQQMLRIAIEVAEHDPLYEDLAV
                     RFLQHTLWIAGAMDRIGEHSDELWDEEDGFFYDVLRLPNGQSQRLKVRSMVGLLPLAA
                     VAVLEESKLEGLPTFRKRSTEFARRHPELCANIHMPSKPGIAGRRMLSVISRDKLRRI
                     LTRMLDENEFLSPYGIRSLSRFHKDNPFVFQANGQEFRVDYLPGESDNGMFGGNSNWR
                     GPIWMPVNFLLIKALYLYFAYYGDSFKIECPTGSGQEMNLMEVAHELSRRLYSIFLPD
                     KDGRRPVYGGTEKFQNDPHFKDKILFYEYFHGENGAGLGASHQTGWTGLVARILRLEN
                     ITNADMLLAPGAASEARKALMAHWQEQ"
BASE COUNT          407 a          477 c          516 g          506 t
ORIGIN      
        1 ccttttaaac agcttatgtt gtctaagatg tactatctat cggcgttttg atagaaattc
       61 tcaataagag cagacctgga ttcgttgacg aaattctgta ctgaatatcg ttttcaatat
      121 cgttttattg ctcttgccag tgggccatga gagcttttct ggcctcgctg gccgctcccg
      181 gtgctagaag catgtcggca ttggtgatat tttccaggcg caaaattctg gctacaagtc
      241 cagtccaacc ggtctgatga ctggctccca gtccggcacc gttttcgccg tggaagtatt
      301 catagaagag gattttgtct ttaaagtgag ggtcgttctg gaatttttcc gtgccaccgt
      361 agacggggcg gcgcccatct ttgtcgggca ggaagatgga gtagagcctt cgggaaagtt
      421 catgggctac ttccatcaag ttcatctcct gaccggaacc ggtggggcac tcgatcttga
      481 aggagtcgcc atagtaggcg aagtacagat acagggcctt gatcagcaag aaattgaccg
      541 gcatccaaat aggtccgcgc cagttggaat tgcctccgaa catgccgttg tcggattcgc
      601 ccggcaggta gtccactctg aattcctgcc cgttggcctg aaatacgaat gggttatcct
      661 tatgaaatct cgacagggag cgaataccat aggggctaag aaattcgttc tcgtccagca
      721 tgcgtgtcag aatgcgcctt aatttgtctc gactgatgac gctcaacatt cgccgaccgg
      781 cgatacccgg tttggacggc atatgaatat tggcgcagag ttccgggtgc cttctggcaa
      841 actcggtgct acgtttgcgg aaagtgggca gtccttccaa ttttgactct tccagtactg
      901 ctactgccgc cagtggcaag agccccacca tggagcggac cttcagtctc tgggactgac
      961 cattcggcag tctcaggaca tcgtagaaga agccgtcttc ttcgtcccac agttcgtcgg
     1021 aatgttctcc gattcgatcc atggctcctg ctatccagag cgtgtgctgc aggaagcgga
     1081 cggccagatc ttcgtagagg ggatcgtgtt ccgccacttc gatggctatt ctaagcatct
     1141 gctggctgaa aaaggccatc caggccgtgc cgtcggcctg ttcgagagag ccgcctgtgg
     1201 gcaaaggcgc actgcggtcg aagaccccga tgttatcgag accgagaaaa ccaccttcaa
     1261 agacattctt gcctttgggg tcttttctgt tgagccacca ggagtagttc actatcagct
     1321 tggtgaaggc gtacttgagg aagtccacat cgccttttcc gttatttcgc actttgtcgt
     1381 gtaaatagac ttcgatggtg gcaaaggcat gcaccggcgg attgacgtct ccgaaattcc
     1441 actcgtaagc cggtatctgg ccgttcgggt gttgatagtc gttgcgcagc atcatgtcca
     1501 gctggtcttt ggcaaaatcg atatcgacgg taccaagggg cactgtgtgg aaggctagat
     1561 cccaggctgc ataccagggg tattcccatt tgtccggcat ggagatgata tcgtcgttat
     1621 acatgtgata ccaatcgctg tttctcacat tggatctttt acttgcccgg ttgagaaagc
     1681 cggcgtcgtg ttccttgagc cagcgctcca cgtcgtaata gaagtattgt ttagaccaga
     1741 gcatgccggc catggcctgg cgaaagaggt tgattttctc ctggtcttct ttcatgtctt
     1801 cggtatagag ggcctggtaa aagccgtcgg cctcctcaat tcgctcagcc acgatatctt
     1861 cgaagtcggc aaagggcttt ggcaacatct ggggggcttt gttgct
//
LOCUS       DAOQNZ010000865         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41852_length_1905_cov_4.737838, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000865 DAOQNZ010000000
VERSION     DAOQNZ010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41852_length_1905_cov_4.737838"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKW73_14240"
     CDS             <1..763
                     /locus_tag="PKW73_14240"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74502.1"
                     /translation="ILEVNPRASRTIPFVSKATGVPWARIAASVMVGKTLSELGITPR
                     LLPPHVAVKSVVIPFKRFAGSQIALGPEMRSTGEVMGIASQFGLAFAKAQLASGHGLP
                     NRGRVFVSVSDSHKQDLIPIAQNLYQLGFELIATRGTAAVIKAAGIPTLTTNKVSEGR
                     PNLVDRIKNGEIQLVVNIPSGRTARDDDQLIRRAAINYNVPVVTTVSGARAVVQGIAA
                     LESGNAGSTGSFAVKSLQDYHQSIAYWETTSNKRV"
     gene            826..1536
                     /gene="deoC"
                     /locus_tag="PKW73_14245"
     CDS             826..1536
                     /gene="deoC"
                     /locus_tag="PKW73_14245"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNA74503.1"
                     /translation="MSKTAEIAKYIDHTLLLPQATKAEVSKLCQEAIQHNFYAVCVNP
                     CFVRQAVKELSSSKVAVATVIGFPLGANLTDTKIAETNKAVSEGAEEIDMVINVGALK
                     GGELDYVTEEIKSVVRAAKNVPVKVIIECDLLSDDEKVQATECCVKAGAAMVKTSTGF
                     VKDGKGATVADIELIKKALAGAKLGIKASAGIRDYAKAKSLIEAGATRLGTSASIEIV
                     KGESGSSPKEDEKTAAAY"
     gene            1550..>1905
                     /locus_tag="PKW73_14250"
     CDS             1550..>1905
                     /locus_tag="PKW73_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74504.1"
                     /translation="MNFRQELKGAAAKVLSTTLCAMTTLALPALAQESEAMKKGEAAY
                     QKMNLKEAANQFREVIRQEPGNAKAHQRLGAVLAAMEDYETAILECKQSIKLDGKNFL
                     PHVVLGQVLANQGKMQ"
BASE COUNT          505 a          537 c          492 g          371 t
ORIGIN      
        1 tattctggaa gtgaatcccc gggcctcccg caccattcct ttcgtctcca aggctaccgg
       61 cgtaccatgg gcacgcatag ccgcttccgt catggtgggc aagacccttt cggaactggg
      121 tattacgccc cgactcttgc cgccccatgt ggccgtcaaa tccgtggtca ttcccttcaa
      181 acgcttcgcc ggctcgcaaa ttgccctcgg tccggaaatg cgctccacag gcgaggtgat
      241 gggcatagcc agccagttcg gtctggcctt tgccaaagcc caactggcct ccggtcatgg
      301 cttgccgaat cgcgggcgag tctttgtcag tgtttccgac agtcataagc aagatctcat
      361 tcccatcgcc cagaatcttt accaactggg cttcgagctt atagccacca gaggcacggc
      421 cgccgtcatc aaggcagcag gcatccccac tctcaccacc aacaaagtct cggaaggcag
      481 acccaacctg gtggaccgca tcaagaacgg cgaaattcaa ctggtggtca atattccctc
      541 cggtcgaacc gccagagacg atgatcaatt gataagaagg gcggccataa attacaacgt
      601 gccggtcgtg actacggttt caggcgccag agcggtggtt cagggcatag ccgccctgga
      661 aagcggtaat gccggcagca ccggcagctt cgccgtcaag agcttgcagg actaccacca
      721 gagcattgcc tactgggaaa ccacaagtaa taagcgcgtg taaggtaagt gccggggctt
      781 aaaggctata atcctgcaaa actcttgttt tacaggactg aaatcgtgtc taaaacagct
      841 gaaatagcca aatacatcga ccatacactg cttctccctc aggctaccaa ggccgaggtg
      901 agcaagcttt gtcaagaagc aattcagcat aacttctatg ctgtttgtgt taatccctgc
      961 ttcgttcgcc aggccgtcaa ggagctttct tcctcgaaag tggcggtagc taccgtaatt
     1021 ggctttcccc tgggcgccaa tctcaccgat acaaaaattg ccgaaaccaa taaagcagtg
     1081 tcggaaggtg ccgaagaaat agacatggtg atcaacgtgg gcgccctcaa aggtggcgaa
     1141 ctcgactacg tcaccgaaga aatcaagtct gtggtgagag cggctaagaa tgtgccggta
     1201 aaagtcatca tcgaatgtga tctgctctcc gatgatgaga aagtacaggc caccgaatgc
     1261 tgcgtaaaag caggagcggc catggtcaag acatccaccg gttttgtcaa agacggcaag
     1321 ggtgccaccg tggccgatat cgagctaatc aaaaaagccc tggctggagc caagctcggc
     1381 attaaagcct cggccggcat tcgcgactac gccaaagcca aatctttaat tgaggccgga
     1441 gccaccagat tgggaacatc cgcctccata gaaatagtca aaggtgaaag cggttcttca
     1501 ccaaaagaag acgaaaagac agccgccgcc tactagcaag gagatcgaaa tgaattttag
     1561 acaggaactg aaaggtgccg cggccaaagt attgtccacc accctctgtg ccatgaccac
     1621 actggctctg ccggctctcg cccaggaaag cgaagccatg aagaaaggcg aagctgccta
     1681 ccagaaaatg aatctgaagg aagcggccaa tcaatttcgg gaagtaatcc gccaggaacc
     1741 cggcaacgcc aaagctcacc agagactggg agccgttctt gccgccatgg aagattacga
     1801 gacggccatc ctcgagtgca aacagtccat caagcttgat ggcaaaaact tccttcccca
     1861 cgtggtgctc ggtcaggtac tggccaacca gggcaagatg cagga
//
LOCUS       DAOQNZ010000866         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41940_length_1903_cov_4.699134, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000866 DAOQNZ010000000
VERSION     DAOQNZ010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41940_length_1903_cov_4.699134"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="PKW73_14255"
     CDS             complement(<1..1629)
                     /locus_tag="PKW73_14255"
                     /inference="COORDINATES: protein motif:HMM:NF024624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
                     /protein_id="HNA74505.1"
                     /translation="MADNGDGKKKASIPAVGWIKLAAFGFIAFIFLGGLAIWMTFGEL
                     VTCLMFPPQWKRDFDVSLAQFRNDDPEAGKRAKKAIEDAKAAGAKPAVLMGMERELGN
                     AFFYNDDSVHGEEEMAAGIALCPPESYKVKGSPDVFEVSQTYQDRAWERHKLHLEDPS
                     KPSGVPDQERSLELAEKNYGAEHVEPAYKLAFLAVLYSENGDFKKAEETMQRTLKLCK
                     RPDIGFETGWYAYAMNARMLAGQGKRKEAFDAYLKGMPLAKTDHQRKRLGSELARGFK
                     YSNSKVPSPFASVLADLKGGNYDALDALDKKLTESKQEKPSGHWLLDEFYETFSENDK
                     AKDGEMDDEQYKQRIADLKNWMSKKPQSTAARLALSETYIKYAWFARGSGYADTVTED
                     GWARYRERIETAKKVLDEDPKIKEKSPRAFDEYGTVALAQGMDKDKYLQLVDECHRLY
                     PTYLDIDLNTCYFLLPRWHGEEQDTEKYIIKRADAIGGGAGDKAYAQMVWFIANKLEA
                     PFAEASLLKWDRTKAGFKQLFKAYPRDSSVPSGFI"
     gene            complement(1651..>1903)
                     /locus_tag="PKW73_14260"
     CDS             complement(1651..>1903)
                     /locus_tag="PKW73_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74506.1"
                     /translation="LKADYGRKVAEDAGYGIGGLALSVGLAASGRLRAIGLLAGGLTF
                     GASTFSSSYHNVKPSQEVLTSIKRKDGDSRLPFLWDNHK"
BASE COUNT          445 a          530 c          426 g          502 t
ORIGIN      
        1 tatgaagccg gaaggcaccg atgagtctcg cgggtaggct ttgaatagtt gtttgaaacc
       61 agctttggtt cggtcccact tcaggagaga agcctctgca aaaggagctt caagcttatt
      121 ggcaataaac cataccattt gagcataagc cttgtcgccg gcaccaccac cgatggcatc
      181 agctctctta atgatgtact tttcagtatc ctgctcctcg ccatgccagc gtggaagaag
      241 gaagtagcag gtgttcaagt caatatccaa gtatgtagga tagagcctat gacattcatc
      301 caccagttgt agatacttgt ctttatccat accctgagcg agagccaccg tgccgtattc
      361 atcaaaggct cgcgggctct tctcttttat tttcgggtct tcatccagca ctttcttagc
      421 agtttcaata cgctcccggt agcgagccca tccatcttcg gttacggtgt ctgcgtatcc
      481 gcttccgcgg gcaaaccagg catacttgat ataggtttcc gaaagagcca gtcgcgccgc
      541 cgtcgattgc ggcttcttgc tcatccaatt cttcaagtca gcgatacgtt gtttgtactg
      601 ctcatcatcc atttctccat ctttcgcttt gtcattctct gaaaaggttt catagaattc
      661 atcaagcaac cagtggccac tgggcttctc ctgcttgctc tcggtcagtt tcttgtccag
      721 ggcatcaaga gcatcgtagt ttcctccttt aagatccgcc agcacagagg caaatggcga
      781 gggaacctta gagttgctgt acttaaatcc acgagccaac tccgaaccga gtcgttttct
      841 ttgatgatcg gttttagcca gaggcatccc cttcaaatag gcatcgaaag cttccttgcg
      901 tttaccctgc ccggccagca tgcgtgcatt catggcatag gcataccaac cggtttcgaa
      961 cccaatgtca ggtctcttgc aaagcttcaa agtgcgctgc atagtttctt cggctttttt
     1021 aaagtcaccg ttttcactgt agagaaccgc taagaaagcc agtttatagg caggttctac
     1081 atgctctgcc ccgtaatttt tctccgccag ctccaaagac cgctcctgat ctgggactcc
     1141 acttggtttg gacggatcct ctaagtgcag tttatgccgc tcccaagctc tgtcctgata
     1201 ggtctgactt acttcaaaca catccggtga accctttact ttgtatgact cgggaggaca
     1261 gagagcaatg ccggccgcca tttcctcttc accatggacg gaatcatcat tgtagaaaaa
     1321 agcattcccc aattcacgtt ccattcccat cagcacggcc ggcttagcac ctgcagcctt
     1381 ggcgtcttct atggctttct tggctcgctt gccagcctcc ggatcatcgt ttctaaactg
     1441 ggccaggctg acatcaaaat cccgtttcca ttgcggcgga aacatcaagc aggtgaccag
     1501 ctcgccaaag gtcatccaga tggcaagccc gcccagaaag ataaatgcaa tgaaaccaaa
     1561 ggcagctaat ttgatccaac caacagcagg aatggacgcc ttctttttac cgtcgccatt
     1621 atcggccata ttcaaatttc cccaatcaga ctatttgtga ttgtcccaca ggaagggtag
     1681 cctggagtca ccgtctttac gtttaatgct ggtcagcact tcttgactgg gttttacatt
     1741 gtgataagag gaagagaacg tggaggcacc aaaagtcaag cctcccgcca gcaaacctat
     1801 ggcccgcaag cgtccactgg cagccagtcc tactgacaaa gccaggccgc cgataccata
     1861 gccggcgtct tcggcaactt tgcgcccgta atcggccttg agg
//
LOCUS       DAOQNZ010000867         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41946_length_1903_cov_3.530303, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000867 DAOQNZ010000000
VERSION     DAOQNZ010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41946_length_1903_cov_3.530303"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..>1903
                     /locus_tag="PKW73_14265"
     CDS             82..>1903
                     /locus_tag="PKW73_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74507.1"
                     /translation="MVRSDSQYSALRGGPRRGSSGGGSGSRGSSGGSSSGRGASGGKR
                     PPPGRGGRGRHDDDEDDFEDDDDDDGESYGLSTPSISGKRMSVTPHASFQNVVAGFYN
                     GLPEKVRELLPWGEEEGESGAEDGPPPDRRGSSSWQEVSFIHSIPGEDEEEGLVVLAD
                     GSFRKYISCKGINALLFDEAEREMMARTFANFANSCESDIQIIIKSRNLSVDEYLSRY
                     QILLKTDNDYLKWYADYTDKWFRKVQDVTFVPQRDFYVVISYHPPDCKTGKSWNGRRS
                     IQKHEEYLEILNRLKKTAFEQLRASNLRPQVLTRKDVRNLIYSDLNPSLYQKDPEAPP
                     SRPNMSEASTLAGSALKITDEYVWLDGKYIGTQYMFATPHETWMGWLVDLLTISTEYT
                     LSMFIHQCDQDRVRRELKLKYRMGHVTSTQLATPDLEGLESTRSAAQAIQEFLRSSNK
                     AFDVSLYLTTAADTPEHLAQNMDEIRRVFKNRGAQMDRGQMLQLDLWQSTLSVGVDKL
                     AVVHRVMSPIVGTMWPFFTASCGTPDGVPFGFALASREPVLLNPFFRGQGKDANNMFV
                     VGTTGAGKSFAVSMMMLRLLPLGTRFVLIDKTVDKFGAYRF"
BASE COUNT          424 a          499 c          528 g          452 t
ORIGIN      
        1 cttaacttag cctcactttc tccaaaagag ccctatgcag ccaagcccaa ttcgaaagat
       61 acgtaataag cggaagagca gatggtaaga agcgatagcc agtattctgc tcttagaggc
      121 ggaccccggc gcgggtcttc cggcggcggc tcgggttccc ggggctcgtc cggtggttcg
      181 tcctccgggc ggggagcgtc cggcggcaaa cgtccaccgc ccggtcgcgg aggacggggg
      241 cgtcatgatg atgacgaaga cgattttgaa gatgacgatg atgatgatgg tgagagttat
      301 ggtctttcga ctcccagtat cagcggtaag cgtatgtcgg ttactccgca cgcttccttt
      361 cagaacgtag ttgccggctt ttataacggg cttccggaga aagtgcggga gcttttgccc
      421 tggggcgagg aagaggggga aagcggcgct gaagacggac cacctcccga taggcgcggc
      481 tccagcagtt ggcaggaagt ttctttcatc cactccatcc ctggagaaga tgaggaagaa
      541 gggcttgtag tgctggcgga tggcagcttc cgtaagtata tttcctgcaa gggcattaac
      601 gccttgctct tcgacgaagc cgagcgcgag atgatggctc gcacctttgc caatttcgcc
      661 aattcatgcg aatccgacat tcagattatt atcaagagcc gcaacttatc ggtggatgag
      721 tacctctctc gctatcagat cttgcttaag accgacaacg attacttgaa gtggtatgcg
      781 gactacaccg ataaatggtt tcgtaaagtt caggatgtga ccttcgttcc tcagcgcgat
      841 ttctatgtgg tgataagcta ccatccgcct gattgcaaga ccggcaagag ttggaacggc
      901 aggcgttcca ttcaaaagca cgaagagtac cttgagattt tgaaccgact caagaagacc
      961 gcctttgagc agttgcgggc ttccaacttg agaccgcaag tactgacacg caaagacgtg
     1021 cgtaacttga tctactcgga tctcaatccc tcgctttatc aaaaagatcc ggaagcgccg
     1081 ccttctcgtc ccaatatgtc tgaggcttct acccttgccg gttccgcctt gaagattacc
     1141 gacgagtatg tctggctgga cggcaagtac atcggcaccc agtacatgtt tgccacgccc
     1201 cacgagacct ggatgggatg gttggttgac ttgcttacca tcagcaccga atataccctg
     1261 tccatgttca ttcaccagtg cgatcaagac cgagtcaggc gtgaactcaa gctcaagtac
     1321 cgcatgggtc acgtcaccag cacccagctt gctactcccg acctggaagg actggagtct
     1381 acgagatcgg cggcccaggc cattcaggag ttcttgcgca gttccaacaa agctttcgat
     1441 gtatctctct atttgaccac tgctgctgac actcccgagc atctggctca gaacatggat
     1501 gaaatcaggc gggtcttcaa aaaccgcggt gcgcagatgg accgcgggca gatgctgcag
     1561 cttgatctgt ggcagtcgac cctctcagtg ggtgtagaca agctggcggt ggtgcaccgg
     1621 gtcatgtcgc ctatcgtggg caccatgtgg ccgtttttca cagcttcttg cggaacccct
     1681 gacggtgtgc ccttcggctt cgcgctggct tcaagagaac cggtgctctt aaatccattc
     1741 ttccgtgggc agggcaaaga cgcgaacaat atgttcgtag taggaacgac cggtgccggt
     1801 aagtctttcg ctgtgtctat gatgatgttg cgtctcttgc cgcttggtac gcgctttgtc
     1861 ttgattgata aaaccgtaga caaattcggt gcttatcggt tta
//
LOCUS       DAOQNZ010000868         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41959_length_1903_cov_3.174784, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000868 DAOQNZ010000000
VERSION     DAOQNZ010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41959_length_1903_cov_3.174784"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PKW73_14270"
     CDS             complement(<1..966)
                     /locus_tag="PKW73_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNA74508.1"
                     /translation="MDKTACITNAVEDDRTGLSIPTLRRALADNLYYQQGKYTAIANK
                     HDYYMMLAYTVRDRLLQRWLNTVQTYLKKDVRVVCYMSAEFLMGPHLGNAMLNMGVEE
                     HFEKALKESGLTMQDLIEEEEEPGLGNGGLGRLAACYLDSLASLEIPAIGYGIRYEFG
                     IFDQEIKDGWQVEITDKWLRYGNPWEIVRPHGAVPVHFGGYTRQYTDAMGRYRVDWVP
                     ERTVTGVPYDTPIPGYDNNTANDLRLWKAEAPESLNLQAFNHGDYAGAVSEKIASETI
                     SKILYPNDNSSQGKKLRLEQQYFFVSATAQTIVRDDFWTSTSNKQL"
     gene            1068..1859
                     /gene="scpB"
                     /locus_tag="PKW73_14275"
     CDS             1068..1859
                     /gene="scpB"
                     /locus_tag="PKW73_14275"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF008506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HNA74509.1"
                     /translation="MDANTASVVSTNIRGKIGIITICNQKKRNALSKAVLDGICLALS
                     EFERSQVTVAVLRAEAGSQVWSAGHDISELPLHTDPLGFFFPLEAALRAVQNFPGPVL
                     AMVEGTVWGGACDLAFTCDILIGEPGSSFAITPVKLGLPYNASGIMHFINRVGLNIAK
                     EMFFTADPIKAERALNLGILNHLVPKQELESFTLAMAERMASHSALSISVIKEQFRLL
                     TNAHGITPESFEMIQGMRRKVYLSHDYAEGIKAFMEKRAPVFEGN"
BASE COUNT          387 a          501 c          540 g          475 t
ORIGIN      
        1 gagttgcttg ttgctggtcg aagtccagaa gtcatcgcgc acgatggtct gcgccgtggc
       61 ggagacaaag aaatactgct gctccaggcg tagtttcttg ccctgggaag aattgtcgtt
      121 ggggtagaga attttggaaa tcgtctcgga ggcgattttt tcagagacgg cgccggcata
      181 gtcgccatga ttgaaggctt gcagattgag cgactcaggc gcttccgcct tccacagtct
      241 caggtcgttg gccgtattgt tgtcgtagcc ggggatgggc gtatcgtagg gcacaccggt
      301 gacggtacgc tccggcaccc agtcgacgcg atagcgcccc atggcgtcag tgtactgcct
      361 ggtataaccg ccgaagtgta ccggcacggc tccgtggggg cgcacaatct cccagggatt
      421 gccgtagcgc agccatttgt cggtgatttc cacctgccag ccgtccttta tttcctggtc
      481 gaagatgccg aattcatagc gtatgccgta accgatggcg ggaatttcca ggctggccag
      541 ggaatccaga tagcaggccg ccagtctgcc cagaccgccg ttgcccagcc ccggctcctc
      601 ttcctcttca ataaggtctt gcatggtcag cccggactct ttgagagcct tttcgaagtg
      661 ctcttccacg cccatgttga gcatggcatt gcccagatgc ggtcccatga gaaactccgc
      721 cgacatgtag cagaccacgc gcacatcctt cttaagataa gtctgtacgg tattgagcca
      781 gcgttgcagc aggcggtcgc gcacggtata ggcaagcatc atgtaatagt cgtgcttgtt
      841 ggctatggcc gtgtacttgc cttgctggta gtaaagattg tcggccagtg cccgccttaa
      901 tgtgggaatg ctcagcccgg tgcggtcgtc ttccacggca ttggtaatgc aggcggtctt
      961 gtccatgttt tggcctctct ggctcgggaa gggagtgatt atatatcttt ctcggtttac
     1021 aagactataa aattgactat gcgcaaaatt cagagggcaa aagagtcatg gacgccaaca
     1081 cggcttcggt tgtaagtact aacatacgcg gcaagattgg cattattacc atctgcaatc
     1141 agaagaagcg caatgccctc agtaaggcgg ttctcgatgg tatctgcctg gccctctcgg
     1201 agttcgagcg cagccaggtg acggtggccg tgctcagggc ggaagccggc agtcaggtct
     1261 ggtcggccgg tcatgacatt tccgaattgc ccctgcatac cgatcccctg ggcttctttt
     1321 ttccacttga ggcggccctg agagccgtgc agaacttccc cggaccggtg ctggccatgg
     1381 tggaaggcac ggtctggggc ggcgcttgcg acctggcttt tacctgcgac attcttatcg
     1441 gcgaaccggg ttcttctttt gccattacac cggtgaaact ggggctgcct tacaacgctt
     1501 ccggcatcat gcactttatc aatcgtgtgg gcttgaacat agccaaggaa atgttcttca
     1561 ccgccgaccc gatcaaggct gaacgcgctt tgaacctggg tattctcaat cacctggtgc
     1621 caaagcagga gcttgaaagt ttcaccttgg ccatggctga gcgcatggct tctcattctg
     1681 ccttgagtat ctcggtgatt aaagaacagt tcaggctctt gaccaatgcc catggcatta
     1741 cccctgagtc ttttgaaatg attcagggta tgcgccgcaa ggtttatttg agccatgatt
     1801 acgcggaagg catcaaagct tttatggaaa aacgcgcgcc tgtgtttgaa gggaattgat
     1861 tttgtccgat tgcgataagt gttcaagacg cagctttctg acc
//
LOCUS       DAOQNZ010000869         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41990_length_1902_cov_3.613969, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000869 DAOQNZ010000000
VERSION     DAOQNZ010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41990_length_1902_cov_3.613969"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKW73_14280"
     CDS             complement(<1..518)
                     /locus_tag="PKW73_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74510.1"
                     /translation="MAGISRKITLLVALPLVFELVFVGTLAVMLHQVSEEREKENYAR
                     DLTSHVNSALTSLLDRFSSCILYHVSDSQLFRKRFEHSRTRINAEISYLRRAVANPEH
                     VAERAMVSRVETLLTQCIEHLQTAQGQLETGNKMLAARQWILADRTISELFVAIDDIV
                     VSQQGQLEKRRAA"
     gene            736..1368
                     /locus_tag="PKW73_14285"
     CDS             736..1368
                     /locus_tag="PKW73_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74511.1"
                     /translation="MVVKSLLLLFLAQTLASGTSPALAQQIATRSGRQLPPCKLDSFV
                     YEAGAHAEHIYGDEGANGLPPYMGFSKAHRINAGIMDVRDAGLTTGHGSLMPDAWGKD
                     EYLGAEWGRSGARGHESSDGFSDGTPIPGGGTGGPGGVDYGPPPGEGYQPATTCEGFV
                     GWYSPEEVALSQTDFPAAFEHFVYSGRYTGSMANAIYILEVQMGRPQGGH"
     gene            complement(1386..>1902)
                     /locus_tag="PKW73_14290"
     CDS             complement(1386..>1902)
                     /locus_tag="PKW73_14290"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA74512.1"
                     /translation="LEKKQRIPDVLLGSGFVFTTYNRDQGFHQQHGAYVNVNMDLPVF
                     YQRQGEIAQAKASLDQSALRLKATSAQVETEVHTAYADLVAARANIFKYQDELIPKAN
                     SVLELAHQGYEKGASDLSNAILAQQSFQTTLVRYFDTVTTYQNAWADLETAVGDIFEF
                     GKGNSDKPPSS"
BASE COUNT          435 a          545 c          529 g          393 t
ORIGIN      
        1 gccgctcgcc gcttttcgag ctgcccctgc tgagaaacaa ctatgtcgtc gatggctaca
       61 aaaagctcgc ttattgtgcg atcagccagg atccactgcc tggccgccag catcttgttg
      121 cctgtttcca gttgcccctg agccgtctgc aagtgctcaa tgcactgggt aagaagggtt
      181 tccacccgtg aaaccatggc ccgctcggcc acatgctcgg ggttggctac ggcccgccgc
      241 aaatagctaa tctcagcatt gatgcgggtc ctggaatgtt caaagcgctt gcgaaaaagt
      301 tggctgtcgg aaacgtgata gagaatgcat gatgaaaacc tgtccagtag agaagtcagg
      361 gccgagttga catgagaagt gagatctctg gcatagtttt ccttctctct ttcttcactg
      421 acctggtgca acatgacggc cagggtgccc acgaaaacca gctcaaagac caggggcaga
      481 gcaactaaga gggtaatttt gcgagaaatg ccagccatag gggagtatta tcgcactgcc
      541 gccgctcagc cgggtcgcca ccaggcagtt ccggcttgcc aaatttttgc caaaagtttg
      601 cccgctggaa gccagcaccg gctcctatat tgtcttcaaa ccggaacgag gtaaccgcca
      661 gagtgcctcc ggttcacaag cacaaagccc tggtcgaaat ttcccgagag cgagactaat
      721 tctatgaaaa gccgaatggt agttaaatct cttctattgc tcttcctggc ccagaccctc
      781 gcttccggca caagcccggc cctggcgcag cagatagcaa cgcgctcggg caggcagctt
      841 ccgccctgca aattggacag ctttgtctat gaagccggcg cccacgccga gcacatctac
      901 ggcgatgaag gagcgaacgg cttgccgccc tatatgggct tcagcaaagc acatcgcatc
      961 aatgccggca tcatggacgt gcgcgacgcc ggtctcacta ccggccacgg cagcctcatg
     1021 cccgatgcct ggggcaaaga cgaatacctg ggcgcggaat ggggtagaag cggcgcccgc
     1081 ggtcatgaaa gcagcgacgg cttcagcgac ggcacgccca tccccggcgg cggcaccggt
     1141 ggaccaggcg gagtagacta tggtccgccc cccggcgaag gttaccaacc tgccactacc
     1201 tgtgaaggct tcgtcggctg gtacagcccg gaggaagtag cactttcaca aacggatttt
     1261 ccggcagcct tcgaacattt cgtctacagc ggccgctaca ccggcagcat ggccaatgct
     1321 atttacattc tggaagtgca gatgggacgc ccacagggcg gtcactgaga accggcggca
     1381 taggctcagg aggacggcgg tttatcagaa ttgcctttgc cgaactcaaa gatgtctccc
     1441 actgccgttt ccaggtcggc ccaggcattt tgataagtgg tgacggtgtc gaaatatcgc
     1501 accagcgtag tttgaaaact ctgctgggcc agaatagcgt tagagagatc ggaagcccct
     1561 ttctcatagc cctgatgggc cagctccagc actgagttgg cctttggaat tagctcgtct
     1621 tgatacttga agatattggc tctcgccgcc accagatcgg cataggccgt atgaacctcg
     1681 gtctccacct gagcactggt ggccttgaga cgcagggccg actgatcgag actggcttta
     1741 gcctgggcaa tttcaccctg ccgttgatag aaaacaggca ggtccatgtt gacgtttaca
     1801 taagcaccgt gttgctgatg gaagccctgg tcgcggttat aggtggtaaa aacgaaaccc
     1861 gaacccaaca aaacatcagg gatgcgctgc ttcttttcca gg
//
LOCUS       DAOQNZ010000870         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_41993_length_1902_cov_3.543584, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000870 DAOQNZ010000000
VERSION     DAOQNZ010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41993_length_1902_cov_3.543584"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKW73_14295"
     CDS             <1..426
                     /locus_tag="PKW73_14295"
                     /inference="COORDINATES: protein motif:HMM:NF037139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA74513.1"
                     /translation="GVLILAATNAPWHLDSAFRRPGRFDRIIFVPPPDTAARASILRI
                     ILKGKPQENIDFEFLAKKTEKFSGADLKALVEDCIEKKIEEAMSKGSEPKPITTKDLE
                     KSARGVKPSTAEWFSSARNYALYSNESGLYDEIAKYLKL"
     gene            426..1793
                     /locus_tag="PKW73_14300"
     CDS             426..1793
                     /locus_tag="PKW73_14300"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74514.1"
                     /translation="MNTHLDRGATLLSHNLYGEAEKELRLALSQNPDSALAHHLLASA
                     LSLQGKLDEALKEGEAALALAPEDDQVYYVLAGIHLHRENLGKAQSCIEQALRFCNDD
                     PDYLSLAAMIQIGKNNWQRARELAETGLAVESEHILCINARAYALARLSQVELARQDL
                     ERALALEPNNDFTHTNMALLLIKGGEIDKAASHLQEALRLDPNSEMAQQALLDAIKSR
                     SPIYHRMLLFTHWLTEMGGKKRVVFILILLLIPALRSLVLLTLLLIWSANHLFTFLLR
                     MDPLGKRILTEQQKRDNTKGVVVVFLLLAFFAFIFLGKSTSRYIAEESSDQLVKQIKE
                     AQINQNQERASKLLERYLKKLRGFLERKRFSVAEVMLTSLDEQKPGLSKDQQLELYLN
                     RLYLYRNGAQDKQAFHQGKEIMVLTELRLFLKKTSPADLAAALKTPAVQAALQRLKVE
                     DPRAL"
BASE COUNT          530 a          519 c          459 g          394 t
ORIGIN      
        1 ggggtgctca ttctggcagc caccaatgca ccctggcatc tggattccgc ctttcgaaga
       61 ccgggacgct ttgatcgcat catcttcgtg ccaccgcccg ataccgcggc gagagcatcc
      121 attttgcgca taatcctgaa aggaaagccg caagaaaata ttgacttcga atttctagca
      181 aagaaaactg agaagttctc aggcgctgat ctgaaagccc tggttgaaga ctgcattgag
      241 aaaaaaattg aagaagccat gagtaaaggc tcagagccca agccaatcac aactaaagac
      301 ctggagaaat cggcccgggg agtcaaacct tccaccgccg aatggttctc ctctgccagg
      361 aactatgcgc tctactccaa cgagagcggc ctttacgatg aaatcgctaa atatctaaaa
      421 ctctgatgaa cacccatctg gatagaggag cgaccctcct ttcccacaac ctctatggcg
      481 aggcggaaaa ggagctaagg ctggcactgt cccagaatcc ggacagtgcg ctggcccatc
      541 acctgctggc aagcgctctg tccttgcaag ggaaactgga tgaggcgctg aaagaaggcg
      601 aagcggcgct ggctctggca ccggaagacg accaggttta ctacgtgctg gccggtattc
      661 atctgcacag agaaaacctc ggcaaggccc agtcatgcat agagcaagca ctccgttttt
      721 gcaacgatga ccccgactat ctatccctgg cagccatgat ccagattggc aaaaacaact
      781 ggcaaagagc cagggaacta gctgaaacgg ggctagcagt ggaatcggaa cacatccttt
      841 gcattaatgc cagagcctat gccctggcca gactctccca ggtggaactg gccagacagg
      901 atctggaacg ggccctggca cttgagccga acaatgattt cacccataca aatatggccc
      961 tattgctcat caagggtggc gaaatagaca aagccgcctc acacttgcag gaagcgctga
     1021 ggcttgaccc caattcggaa atggcacagc aagccctgct ggatgccatc aagagccgca
     1081 gtcccatcta ccacaggatg cttcttttca cccactggct tacggaaatg ggcggcaaga
     1141 aacgtgtggt cttcattctt atcctcttac tgattccagc cctgagaagc ctggtgcttt
     1201 tgacccttct gctaatctgg tcagccaacc atctatttac cttcctgttg cgaatggatc
     1261 ctctcggcaa acgtatcttg acggagcaac agaagcgcga caatacaaaa ggagtggtgg
     1321 tcgtatttct gctcctggcc ttcttcgcct ttatcttcct gggcaagagc acctctcggt
     1381 atattgcgga agaaagttcg gatcagcttg taaaacagat caaagaagcg caaataaatc
     1441 aaaaccaaga gagagcatcc aaattgttgg aacgctatct caaaaagctg agaggtttcc
     1501 ttgagcgcaa acgtttcagc gttgccgagg taatgcttac cagccttgat gaacaaaaac
     1561 cgggcctatc aaaggaccag cagttggaac tatacctgaa ccgattgtac ctatatagga
     1621 atggggcaca ggataaacag gccttccacc aggggaaaga aatcatggtg ctgaccgaac
     1681 tccggctctt cctgaagaaa accagcccag ccgatctggc agcagccctg aagactcccg
     1741 cagtgcaggc cgcgctccaa cgactgaaag ttgaagaccc tcgggctttg tagcgcactt
     1801 tcacgaaacc ttcacacctc ataagccata ctcaaaacct tgaccggttc cacgcttact
     1861 aagaatcaag aagtgagttt ttcttatgac agccaaagac ac
//
LOCUS       DAOQNZ010000871         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42042_length_1901_cov_3.317443, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000871 DAOQNZ010000000
VERSION     DAOQNZ010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42042_length_1901_cov_3.317443"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKW73_14305"
     CDS             <1..445
                     /locus_tag="PKW73_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA74515.1"
                     /translation="ASNQPPYSMVLRDIEKDIVPYCLAHKISLLVYSPLQRGLLTGKF
                     KPDAVFKDGDHRGKQSHFQPENIKRTNAFLERIKPIAEDHGATLGQLVIAWTIAQPGI
                     TAAIVGARDAAQTVENLKAAGIELSATELATINRELETLTLVAAS"
     gene            511..1782
                     /locus_tag="PKW73_14310"
     CDS             511..1782
                     /locus_tag="PKW73_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74516.1"
                     /translation="MSEKILDGQPDAPSGAGLAQRSLSEKLVDQGELFWGALKQAPDS
                     FKAAGQEALSKPGDTALMTAASFGLGATVALLSKRPDLLGQTVKPLIENTLHNGGKVL
                     SGTALVDLTGKTVAPSYAVWKDPGKLESSQALLGSRLGRGAFDYGLTLSSGAAGFLSV
                     SRLGHAGLHPSAIGAGFEGLHRQGEVLKQLPASVRAENFLGRGGNGAVYKLDFTDDFV
                     VKVPEFKTKTPLLGELEKVDDIMPGANIGQPVAKMGDYLILKKQNGFPAGSPDARSRR
                     AMSPEAAEAVYARSIQESAALPQSAYDELAVLMMKLEKRGLQFDPSKPGNILIDSAAR
                     RFNLVDISPTSAVRPYQHTVSDMIVPLVDNYFTGSVLSDKGLKYRPQLAEIITKATKA
                     SLNASMHPVREGDSSLRYSYQLAGLKPPEAL"
BASE COUNT          411 a          525 c          524 g          441 t
ORIGIN      
        1 ggcctctaat cagccgcctt acagtatggt tctgcgcgat atagagaaag atattgtgcc
       61 ttattgcctg gcgcataaga tctcgcttct cgtctacagc ccgctgcaga gggggctttt
      121 gaccggcaag tttaagcccg atgcagtctt caaggacggt gatcatcggg gcaagcagag
      181 ccattttcag cctgagaaca tcaaacgcac caatgctttt ctggaacgca tcaaacccat
      241 tgccgaggac catggcgcca ctttaggtca acttgttata gcctggacca ttgcccagcc
      301 cggtatcacc gccgccatcg tcggcgccag agacgcggcg cagactgttg agaacctcaa
      361 agcagccggc attgaattga gcgccaccga gcttgccacc attaatcggg aactggaaac
      421 actcaccctt gtggcggcat cctgagtttc gcctgacttt ctgcgaggca taaagcagga
      481 aggaaataga ttctcagcgc gagttgcagc atgagcgaga aaattttaga tgggcaaccg
      541 gatgctcctt cgggcgctgg tcttgctcaa cgctcgcttt ctgagaaact tgtcgatcaa
      601 ggtgagctat tctggggagc cttgaagcaa gccccggact cttttaaggc agccgggcag
      661 gaagctcttt ccaagcccgg cgacactgcc ttgatgacgg cggctagctt cggtcttggt
      721 gcgaccgtgg cactcttaag caagcgtccc gatctgcttg gtcagaccgt gaagcctctt
      781 atcgagaaca ccctccataa tggcggcaag gttttgagcg gtacggcgct ggtagatctt
      841 accggcaaga cagtcgctcc ctcttatgct gtttggaaag acccgggcaa actggagagc
      901 agtcaggcac ttctggggtc tcgcctgggc cggggtgctt tcgattacgg tttgaccctt
      961 agctccgggg ccgccggctt ccttagtgtt tccaggctgg gccatgccgg tttgcatccc
     1021 tcagcaatcg gcgccggctt tgaaggtctg caccggcagg gggaggtgct caagcagctt
     1081 cctgccagtg tcagggcgga aaacttcctt ggacggggcg gcaacggtgc cgtctacaag
     1141 ctggatttta ccgacgactt tgtggtcaag gtgcctgaat ttaaaactaa aactcctcta
     1201 ctgggtgagc tggaaaaggt tgacgacatc atgcccgggg ccaatatcgg gcagcccgtg
     1261 gcaaaaatgg gcgactattt gattttgaag aagcaaaatg gttttccggc tggctctcct
     1321 gatgccagaa gtcgccgtgc catgagtccc gaggccgccg aggcggttta tgcacgcagt
     1381 attcaggaaa gtgctgcgct gcctcagtca gcctatgatg agctggctgt gctgatgatg
     1441 aagctggaaa agcgcggtct gcaattcgac ccttctaagc ccggtaacat cttgatcgac
     1501 tctgccgcca ggcgcttcaa cctggttgat atcagcccga ccagtgcggt cagaccttat
     1561 caacacacgg tctccgatat gatcgtgccc ctggtcgaca attactttac cggttcggtt
     1621 ctcagcgata aagggctcaa gtatcgcccc caactggctg agataatcac taaagctaca
     1681 aaagcgtccc taaatgcatc catgcacccc gtccgcgaag gtgattcctc tttgcgctat
     1741 agttatcagt tagcaggttt gaagccgcct gaagctcttt gaaagttgga gtgccattgt
     1801 ccgatcttcc cagccagatc aaagccgatg ccgacggcgc caccttgctg gctccttacg
     1861 gtaagagccg ttattcactt gagctttcca tgtccggtgc c
//
LOCUS       DAOQNZ010000872         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42079_length_1900_cov_3.872087, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000872 DAOQNZ010000000
VERSION     DAOQNZ010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42079_length_1900_cov_3.872087"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PKW73_14315"
     CDS             <1..1185
                     /locus_tag="PKW73_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74517.1"
                     /translation="NQKWVQKNRICMVLSLGDLQIERDILNRDEHFWPVLAIDGDLRA
                     VNIVKGGMPLLIWGNLELAGYMVPDYNDGPLRLGKDLRALGYVPACRDSKNGRGHVIG
                     GKVLAKVLDAREGLSGKELRRLLVPGTFQKSWLDNDAVLAFGHEGRDIFRDEPLLEEP
                     QPQPEPVEILAPDGVDPTALGTIESPDMVLPMLLCRISEKIEYDPDRYSYPESFAEFV
                     RSHFEYHKSMKVLTLPGGSVIKGDLLLDFQEAWAMEKNISAVVCDGHLTVAGDVLNSM
                     LEGGILLFVSGNLQVENLIKGGATVMVLGDVLAGGLVIGVYNDGVMRIGGDLTASAYF
                     LLDHDGYVRGKVEALTHTDDDGDWCELLLPHVFDDEEDFHPNVDKIRACQKTGRAIFA
                     SV"
     gene            1252..>1900
                     /locus_tag="PKW73_14320"
     CDS             1252..>1900
                     /locus_tag="PKW73_14320"
                     /inference="COORDINATES: protein motif:HMM:NF016705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNA74518.1"
                     /translation="MSPSKKSVLVLGVAALGLGIYGAGAALAHYEGPPYEVLKSDGKF
                     EWRRYKPRIVASVDVEGEYRQSMNKGFGILAGYIFGKNVSRSKVAMTSPVLVASADSQ
                     ASLKSERQGEIEKIAMTAPVVVENEPGRQAMTMRFFMPEKYSLADLPTPVDERIRLST
                     LPEQTFVVVRFSGSWKQSEFDKRAKELQTYAAGAGLKVTGEPASAFYNPPFTPPFL"
BASE COUNT          390 a          467 c          568 g          475 t
ORIGIN      
        1 aaccagaaat gggtgcagaa aaatcgaatt tgtatggttc tttctcttgg tgatttgcag
       61 attgaaagag atatcttaaa ccgcgacgag cacttctggc ctgtgcttgc tatagacgga
      121 gatcttcgcg ccgtcaatat agtcaaaggc ggtatgccgc ttctgatctg gggtaatctt
      181 gagcttgccg gatatatggt gcccgattac aacgatggac cccttcgtct cggaaaagac
      241 ctgagggccc tgggttatgt gccggcatgt cgcgacagta agaacggtcg cggccatgtt
      301 atcggcggca aggtgctggc caaggtgctt gatgcgcggg agggactctc gggcaaggaa
      361 cttcgccggt tgcttgttcc cgggactttc cagaaaagct ggctcgacaa tgatgcagtg
      421 ctcgccttcg gccatgaagg gcgggatatt tttcgagacg aaccgctact tgaggagccg
      481 cagccgcagc cggagccggt tgagattctt gcacctgatg gcgtcgaccc gaccgctttg
      541 ggtaccattg aaagccccga catggttctg ccgatgctgc tgtgtagaat cagtgagaag
      601 attgaatatg acccggaccg ctactcctat ccggagagtt ttgcagagtt tgtaaggagt
      661 cactttgaat accacaagtc gatgaaggtg ctgacgctgc ctggtggcag cgttatcaaa
      721 ggtgacttac tgctcgactt tcaggaagcc tgggcgatgg agaagaatat atcggcggtt
      781 gtctgcgatg gccacctgac tgtggccggc gatgtgctca attccatgtt ggagggaggc
      841 atcctgctct ttgtctcagg caatttgcag gttgaaaatt tgatcaaggg cggcgctact
      901 gtcatggttc ttggtgatgt cctggccggc gggctcgtca tcggtgtcta taacgacgga
      961 gtgatgcgca tcggtggtga tctgacggct tctgcctact tcctgctcga tcatgacggc
     1021 tatgtgcgcg gcaaggtgga agcgctcact cataccgacg atgacgggga ttggtgtgag
     1081 ctgcttttgc ctcatgtctt tgacgatgaa gaggacttcc atcctaatgt cgataagatt
     1141 cgtgcctgcc agaagaccgg ccgggctatt tttgcctccg tttgagctgg ttcacctcaa
     1201 gttcacccgc gcctgcttta attgagatat cgagggttag aggttgtttc catgtcgcct
     1261 tctaaaaagt ctgtgcttgt cctgggggtt gccgctctcg ggcttggtat ctacggtgcc
     1321 ggcgcggcac tggcccatta tgaaggacct ccttacgagg tgctcaaatc cgacggcaag
     1381 ttcgaatggc gccgctacaa gccgagaata gtggcttccg tagatgtgga aggtgagtat
     1441 cggcagtcta tgaacaaggg gtttggcatt cttgccggct acatcttcgg taagaatgtc
     1501 tctcgcagca aagtggccat gaccagtccg gtgcttgtgg ccagcgctga ttcgcaagca
     1561 tccctcaagt ctgagcggca aggtgagata gagaagattg ccatgacggc gccggtggtg
     1621 gttgaaaatg agcccggccg ccaggctatg accatgcgtt ttttcatgcc tgaaaagtac
     1681 agcctcgccg atttgcctac ccccgtcgat gagcgcatca ggttgtctac cctgccggag
     1741 cagacctttg tggtggtgcg attttcaggt agttggaagc agtcggagtt tgataagcgc
     1801 gccaaagaac tgcagaccta tgccgctgga gccgggctga aagttacggg tgagccagct
     1861 tctgcctttt acaatccacc cttcacgccg cccttcctgc
//
LOCUS       DAOQNZ010000873         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42143_length_1899_cov_3.236443, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000873 DAOQNZ010000000
VERSION     DAOQNZ010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42143_length_1899_cov_3.236443"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKW73_14325"
     CDS             <1..525
                     /locus_tag="PKW73_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74519.1"
                     /translation="RVLILMKEKPLCHQTNPKFLYRLLVLGPGFNNAASCFHERLTEE
                     GTLLKYTTISSEPEFPFADSESGNESKIALQSRKIEISRSQVAELNSLLEEAESYYAK
                     NKYISYEMIPAARHYLLEFQVKGRYYYYDFEGKDPGNSACTKEALLPQFLKGLLARNR
                     SLCKDYETSLTVPQ"
     gene            complement(508..1197)
                     /locus_tag="PKW73_14330"
     CDS             complement(508..1197)
                     /locus_tag="PKW73_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74520.1"
                     /translation="MLAISGNLPALADGLVNMGHISGPHTRLLLTSSQKLSLDKGARS
                     IDLTGAQKKALGHIASAKNVGRLSVLPKTIQTCTCELSNVSIRLDRDSIEVADNLLGR
                     TFASPLEVRFRSRQRSWQLAQKDAPLSQPPKAEELLNARGNEAGSSIERSISFYKQAL
                     AVNPNYVLARRNLANCYSQQAWHSLMEKRDSKKACRLFELALANAEPSDEVFRARTES
                     DLSFARSLGDR"
     gene            complement(1248..>1899)
                     /locus_tag="PKW73_14335"
     CDS             complement(1248..>1899)
                     /locus_tag="PKW73_14335"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNA74521.1"
                     /translation="ADMVVNTLRNVVLGVDRIRLCIVEHFLCAAVLWGLDDAYVYVDG
                     PEMPLGDGSSEFWIRLFEASGIARGAVSADLELKETITVERGDRSLIAIPAEKFSVTY
                     LMDWKHPLIGQKWQSWDVSRDIKEVYEARTFGMLKEHEMLGLDKDVVSLTEDGFTMPL
                     KSADEPVRHKLLDLIGDLALIGINPLRIKARFISIKGGHEMDVDIARKLASVLGLQ"
BASE COUNT          519 a          499 c          431 g          450 t
ORIGIN      
        1 agagttctaa ttctcatgaa agaaaagcct ctctgtcatc aaaccaatcc caagttcctt
       61 taccgactcc tggtacttgg tcccggcttt aacaatgccg catcttgctt tcatgagcgg
      121 ctgactgaag aaggtacgct gttaaaatac accacaatca gcagcgaacc tgaatttccc
      181 ttcgcggata gtgaatcagg caatgagtca aagatagcct tgcaaagcag gaagattgaa
      241 atatccagaa gccaggtagc tgagctgaac tccttgctgg aagaagctga aagctattac
      301 gctaagaaca agtacatctc ctatgaaatg atcccggcag ccagacacta tctcctggag
      361 tttcaagtta agggtcgcta ctactattac gactttgagg gaaaagaccc gggaaattcc
      421 gcctgcacta aagaagcgct tttgccgcaa ttcctcaaag gtctgctggc aagaaaccgc
      481 tctctttgca aagactacga aacctcatta acggtccccc aatgacctgg cgaaagagag
      541 atcgctctcc gtgcgcgctc gaaatacttc atcactgggt tcggcatttg ctagtgccaa
      601 ttcgaagagg cggcaagcct ttttggaatc acgcttctcc atgagactat gccaggcctg
      661 ctggctataa caatttgcca gattgcgcct ggctaaaaca tagtttgggt ttacggcaag
      721 ggcttgctta tagaaagaga tagaccgctc aatagaagaa cctgcctcat tgcctctagc
      781 attcaataat tcttccgcct ttggtggttg agagagagga gcgtcctttt gtgctaactg
      841 ccaggagcgc tgacgtgacc tgaaccttac ctccaaggga gaggcaaatg tgcgtccaag
      901 taggttatcc gccacttcaa ttgaatctcg gtccaatcta atcgagacat tactcagttc
      961 gcaggtacag gtctgaatcg ttttaggaag cacagaaagt ctgcctacat tctttgcaga
     1021 agcaatatgt ccaagagctt tcttctgcgc gccggtcagg tctatagacc tggcgccttt
     1081 gtctaaagaa agcttctgag aactggtgag aagcaacctg gtatgaggtc cactaatgtg
     1141 ccccatgttt acgagtccat cggccagggc cggcaaatta ccagatattg ccagcaaaaa
     1201 tgtaatcacg caggcaggaa aattcgctct caaatcaaat tgagctccta ctggagcccc
     1261 agtacggaag ctagtttacg ggcgatgtcc acatccatct cgtgaccgcc cttgatactg
     1321 ataaagcgag ccttgatgcg gaggggattg atgccgatca gtgccagatc gccaataaga
     1381 tcaagaagct tgtgacgcac cggctcatcg gcagatttaa gcggcatggt aaatccgtct
     1441 tccgtgaggc tgaccacgtc tttgtcgagc ccaagcatct cgtgctcctt gagcatgccg
     1501 aaggtacggg cctcatagac ttccttgatg tcccttgaaa catcccagct ctgccatttc
     1561 tgaccaataa gaggatgctt ccaatccatc aagtaggtaa cggagaactt ctcagccggt
     1621 atggcaataa gacttctatc gcctctctct acagtaatcg tctccttcaa ttccagatca
     1681 gccgacaccg cacctctggc gataccacta gcttcaaaga gacgaatcca aaattcagag
     1741 cttccatctc ccaaaggcat ctcgggccca tctacgtaga cataagcatc atctagcccc
     1801 caaagcacag ccgcgcatag aaaatgctcg acgatgcaaa gcctgatgcg gtcgacacca
     1861 agtacgacat tgcgcaaagt atttacgacc atgtcagct
//
LOCUS       DAOQNZ010000874         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42233_length_1897_cov_2.785559, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000874 DAOQNZ010000000
VERSION     DAOQNZ010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42233_length_1897_cov_2.785559"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="PKW73_14340"
     CDS             <1..1504
                     /locus_tag="PKW73_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA74522.1"
                     /translation="IQKKEPRPVKGKVRAGLIVLALLGLGAGGYYYTTSSQKAAETVE
                     AQKNLFEVKTGRAKITVLATGVIKPDREVKLSPKQTGLLKALLVKQGDRVKEGQLIAV
                     MDDSNLLGEVAAARGAYLASLDNVNKLKSGNRPQEVAQARYQELKAKNAARQARENIQ
                     RLEAQVESLKIQLNRNETYAKRQTMLAKEGAVSDQAGIDADMAADVTRSQLRAAVQEK
                     EQAEAALAQSLAELSSITEQHNLMRSGFRKEDIAAAEHNSMQTKGQLMRMESLLNDTR
                     IKAPFAGVITQKYADAGAIVTPTTSAATTSATSSSIVALAGKLEMVAQVSESNLPKIQ
                     IGQPVEITATAFPDRIFHGRVTQIAPAAIVTSNVTTFEVHAEPVDDNKGDLLAGMNVS
                     AKFIVGELENAMTVPTVCVISRKGQSGVFLPDKKGEPEFKAVKTGPAVGRDIVILEGL
                     KPGDKIFQGLSREQLAKEGYGGRMPGATGGPGASAMRMGGGNRMGRGMGP"
     gene            1508..>1897
                     /locus_tag="PKW73_14345"
     CDS             1508..>1897
                     /locus_tag="PKW73_14345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA74523.1"
                     /translation="MNLRELLGIAWQGILSNKLRSGLTVLGIVIGIASVITLLGIGQG
                     AKLEAEKQVQSLGVNLIYVRPGAANLSSISQGQGSAATLTYDDAQAIRENCPAVEDVA
                     AQYNSAFQVQRGEQNTSTSVVATEPSYT"
BASE COUNT          487 a          559 c          526 g          325 t
ORIGIN      
        1 gattcagaag aaagaaccgc gaccggtgaa aggcaaagtt agagccgggc tcatcgtatt
       61 ggctctactg gggcttggcg ccggcggcta ctactacact acaagcagcc agaaagcggc
      121 tgaaaccgtg gaagcacaga agaatctctt tgaagtgaaa accggtagag ccaaaattac
      181 agtgctggcc accggcgtca tcaaaccaga ccgagaagtg aaactcagcc ccaagcaaac
      241 cggcttgctg aaagccctgc tggtcaagca aggggaccgg gtgaaagaag gtcaactgat
      301 agcggtgatg gacgacagta atttacttgg agaggtggcg gccgctcggg gagcctatct
      361 ggccagcctc gataacgtca ataaactgaa gtcgggaaac cgcccccagg aagtggccca
      421 ggctcgctac caggagttga aggccaaaaa tgccgcccgt caggccaggg aaaatatcca
      481 gcgcctggaa gcccaggtgg aatctctcaa gattcaactg aatcgcaatg aaacctatgc
      541 caaacgccag accatgctgg ccaaagaagg ggccgtttct gatcaagccg gcatcgatgc
      601 cgacatggca gccgatgtca cccgctccca gttgagagct gcggtgcaag agaaggaaca
      661 ggcggaagcg gctctggccc agagcctggc tgagctatcc tccatcaccg agcagcacaa
      721 tttgatgcgc tcgggtttcc gaaaagaaga cattgccgcc gccgagcaca attccatgca
      781 aacaaaaggg caactgatgc gcatggaaag tctcttgaat gacacccgca tcaaggctcc
      841 ctttgccggg gtaatcactc aaaaatatgc cgatgccgga gcgatagtga cgcccaccac
      901 ttctgccgcc accacctccg ccacatccag ttccattgtg gccctggccg gcaagctgga
      961 aatggtggca caagtatccg aatccaatct gcccaagatt caaatcggac aaccggtgga
     1021 gataacagcc acagcatttc cagaccgcat cttccacggc cgggtaacgc aaatcgctcc
     1081 ggcggccata gtcacctcca acgtcaccac cttcgaagtg cacgccgagc ccgtcgacga
     1141 caacaaaggc gacttactgg cgggcatgaa tgtgagcgcc aaatttattg tcggcgaact
     1201 ggaaaatgcc atgaccgtgc ctacagtctg cgtcatctcc cgcaaaggac agtccggggt
     1261 cttcctgccg gacaagaaag gcgagcctga attcaaagct gttaaaaccg gccctgctgt
     1321 aggcagagac attgtaattt tggaaggact taagcccggc gacaaaatct ttcaaggtct
     1381 cagtcgcgag caactggcta aagaaggtta tggcggtcgc atgcccggcg ccaccggcgg
     1441 tcccggcgcc tcggccatgc gcatgggcgg cggcaaccgc atgggcagag gcatgggccc
     1501 ctagtttatg aatctgcgtg aactactggg catagcctgg cagggcatcc tgagcaataa
     1561 attgcgctcc gggttgaccg tgcttggtat cgtcatcggt atcgcctccg taatcaccct
     1621 tctgggcatc ggacaggggg ccaaactgga agcagaaaag caagtgcaat cactgggcgt
     1681 caacctcatt tatgtgcgtc ccggtgccgc caacctgtcg agcatctccc agggtcaggg
     1741 ctccgccgcc accctcacct atgacgacgc ccaggccata cgcgagaact gcccggcggt
     1801 ggaagacgtg gccgcccaat acaactccgc cttccaggtg caacgcggcg agcaaaacac
     1861 atccacctcg gtggtggcaa ctgaaccctc ctacacg
//
LOCUS       DAOQNZ010000875         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42425_length_1893_cov_3.115887, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000875 DAOQNZ010000000
VERSION     DAOQNZ010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42425_length_1893_cov_3.115887"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKW73_14350"
     CDS             complement(<1..526)
                     /locus_tag="PKW73_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA74524.1"
                     /translation="MQYRQLGESDLQVSSIIFGAWAIGGWMWGGAEEKDAIEAIHASI
                     DGGATSIDTAAVYGYGRSEEIVARAVSGRRDKVQLLTKYGLRWDATEGEHFFDWSDEQ
                     GTRHIYRNARKKSIIAECEASLRRLNTDYIDLYQCHWRDPSTPVEETMEAMNELVAQG
                     KVRAVGVSNFCADDI"
     gene            689..1504
                     /locus_tag="PKW73_14355"
     CDS             689..1504
                     /locus_tag="PKW73_14355"
                     /inference="COORDINATES: protein motif:HMM:NF003703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNA74525.1"
                     /translation="MQIVNISAYKFQAVEDPANWQPIFKTEADRLGLKGTILLSPEGI
                     NLFLAAPQDTLDQFMTFLRQDIYLGDDFGRQAVVGYSDHQPFGKMVVRLVKEIITMRH
                     PTINPSGQRAPAVSPEKLKEWLDRGHDDEGRPVVMLDTRNAFEVAIGTFENAQHFNIE
                     RFSQFPDALKEDLAKESESARSLKEKTVVSFCTGGIRCEKAALFMQELQLPRVYQLDG
                     GILRYFEKVGGAHYQGECFVFDERVALDPSLKETVRDYEGRTKKSTAQCQLPG"
     gene            1513..>1893
                     /locus_tag="PKW73_14360"
     CDS             1513..>1893
                     /locus_tag="PKW73_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74526.1"
                     /translation="MKSILRRSLLAIVSVFAITGSATAAEGHWKERALPKLQPSRAAT
                     ALSYKDQPVDTAAAENREPLVVLEDYGIACASFYARRDGFNLPYGRAFAGAEARPRLR
                     LGLARKLQQVNESLKGQGLEVLVLD"
BASE COUNT          456 a          532 c          502 g          403 t
ORIGIN      
        1 cgatgtcatc ggcgcagaaa ttgctgacgc ccacggcccg cactttcccc tgggccacaa
       61 gctcattcat ggcttccatg gtctcttcca caggggtgga agggtcgcgc cagtggcact
      121 gatacagatc gatataatcg gtgttcaggc gccgcaaact ggcttcgcat tcggcgatga
      181 tgctcttctt gcgggcattg cgataaatgt gcctggtacc ctgctcatcg ctccagtcga
      241 agaaatgctc accctcagtg gcatcccagc gaagcccata cttggtgaga agttgcacct
      301 tgtcgcggcg tccactcacg gctctggcca ctatttcttc ggaacgtccg taaccgtaaa
      361 cggcagcggt atcgatggaa gtggcaccac catcgataga agcgtgaatg gcctcgatgg
      421 cgtccttttc ctcggcgccg ccccacatcc agccaccaat cgcccaggca ccgaagatta
      481 tgcttgaaac ctggagatca gactctccca attgacgata ttgcatttgt ttacccaaac
      541 tttagtgacc gaggtccctg agaaaactgc aattatatag gcattgcgac aatctgagcg
      601 agacggaaga cctggcacct gagaacaatt ttctcttaga cctccagcct ggcgctcaaa
      661 ccaggcaatt tgtcaatata ataactccat gcaaatcgtc aacatttccg cttacaaatt
      721 ccaggctgtc gaagatccgg caaattggca gcctatcttc aaaaccgagg ctgaccgcct
      781 tggtttgaaa ggcactatcc tgctatcccc ggagggaatc aatctcttcc tggccgcacc
      841 gcaagacacc ctcgaccagt tcatgacctt ccttcggcag gacatttatc tgggcgacga
      901 ctttggcaga caggcagtgg taggctattc cgaccaccag cccttcggca aaatggtggt
      961 cagattggtg aaagaaatca tcaccatgcg tcaccccacc attaatcctt ccggtcaaag
     1021 ggcaccggcc gtcagccccg agaagctgaa agaatggctg gatcgcggtc atgatgatga
     1081 aggacgcccg gtggtcatgc tcgacacgcg caatgctttt gaagtggcca taggaacttt
     1141 tgaaaacgcg cagcatttca acattgagcg cttcagccag tttcccgatg ccctgaaaga
     1201 ggacctggcc aaggaaagtg aaagcgcccg ttcactgaag gaaaaaacag tagtctcctt
     1261 ttgcacaggc ggtatacgct gcgagaaagc agcccttttc atgcaagagc tgcagttacc
     1321 ccgagtctac cagttggacg gcggcatcct tagatatttc gagaaagtgg gcggcgctca
     1381 ctatcaaggc gaatgctttg tctttgatga aagggtagct ctcgatccct ccctcaagga
     1441 aacggtcagg gattacgaag ggcgcaccaa gaaaagtacg gcacagtgtc agttgccggg
     1501 ctaagggcaa tagtgaaaag tatattgcgg cggtccttgc ttgccatagt gtccgtcttt
     1561 gccattacag gcagtgccac tgccgccgag ggccactgga aagagcgggc tctgccgaag
     1621 ctgcaaccct ccagagcggc aaccgctctt tcttacaaag accaaccggt cgatacggcc
     1681 gccgccgaaa accgtgaacc actggtggtg ctggaagact acggtattgc ctgcgcctcg
     1741 ttctatgctc gccgggatgg tttcaatctc ccttacgggc gggcatttgc cggggcggaa
     1801 gccaggccga gactgcgact ggggctggca cgcaaactgc agcaagtaaa cgaaagcctg
     1861 aaagggcagg ggctggaagt gctggtactg gac
//
LOCUS       DAOQNZ010000876         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42470_length_1892_cov_3.168753, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000876 DAOQNZ010000000
VERSION     DAOQNZ010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42470_length_1892_cov_3.168753"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..1323
                     /locus_tag="PKW73_14365"
     CDS             187..1323
                     /locus_tag="PKW73_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA74527.1"
                     /translation="MQMHLERLDFRLQLKALIRKELQQILRDRQLLFLLFAMPILQLF
                     IYGFALSPEVDRLRLGIVDHAGSTISRALAACLVVNGVFEVEESGGTEAQLARKVQDG
                     QLDAGLIIPADLERRFRSGQKTRVQVLIDGVDANTAGIAGGYVGQILAAFNRQLTTGT
                     QLPRELVAPQITFAYNPGLISSWFFVPGVIALVLNLVSTLVSSAALVREKDSGTLEQL
                     LMTPVSSLQILLAKIVPLSFLLMITVFVALMVAFLIFQVPFRGNLLLFLFVSFLAILV
                     GISIGIALAAYSENQRQSLLTSFYVNLPVIQLSGAIAPLESMPEIFQWMSLLNPLRYY
                     VSCVRSIILKGVGLEEIWPNVLALAAYALILLSLSSSRFRKQLK"
     gene            1320..>1892
                     /locus_tag="PKW73_14370"
     CDS             1320..>1892
                     /locus_tag="PKW73_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA74528.1"
                     /translation="MMMRSKLKLTRLLLLLSCSQSLCFGPGPALAAAPAVSDGQADGR
                     LQADLNAAEPVSTDDLSGDALNPERIDLKRPSYKALISIDQKIDPFVLDATGSRGIGL
                     ADVVDATLKTNLDIGIADYDERIKRTNLLGSYGKFLPDLSLSYQYNYLKGKANVPFGA
                     SLEPLAFNNPLIITSAGFKYYGYRGGSVLFG"
BASE COUNT          356 a          497 c          502 g          537 t
ORIGIN      
        1 ctccctcatt tccaacatat taccggcgcg ctatttcgtt gaagaatgtc gcaatgcctt
       61 cgtgcgcggt gccgacttcg caagccagct ctatataccg cttgccctat cgagttgcgc
      121 actcttcctc tatctggtca catccaatat cctgcgcaaa atgcagttga aggggtagga
      181 ggatctatgc agatgcactt agagcgcctc gattttcgcc ttcaactgaa ggctttgatc
      241 cgcaaagaat tgcagcagat cttaagggac cggcagctct tattcttgct ctttgccatg
      301 cccattttgc agctttttat ctacggcttt gccttgagcc ccgaagtaga ccggctgcgc
      361 ctgggcattg tcgaccatgc cggttccacc atcagccggg ctcttgcggc ctgcctggtg
      421 gtgaatggcg tctttgaggt ggaagaaagc ggtggtacgg aggcgcagct agccaggaag
      481 gtgcaggacg ggcaactgga tgccggcttg ataattcctg ccgatcttga gcgccgcttt
      541 cgatccggac agaagaccag ggtgcaggta cttattgatg gtgtggatgc caatacggcc
      601 ggtatcgccg gtggctatgt ggggcagatt ttagcggcat tcaacaggca gttgactacc
      661 ggtacgcaac tgcccagaga actggtggca ccgcaaatta cctttgccta taatcctggg
      721 cttatcagca gttggttctt tgtgcccggg gtaattgctc tggtgctgaa tctggtatcg
      781 acactggtca gcagcgccgc ccttgtgcgc gagaaagact cgggcaccct ggagcagttg
      841 ctcatgacgc cggtcagttc tctgcaaatt ctcctggcga aaattgtgcc gctctctttc
      901 ttgctcatga ttaccgtttt cgtggctttg atggtagctt ttctcatttt tcaggtgcct
      961 tttcggggca atcttttgct ctttcttttt gtctcttttc tggccatttt ggtgggcatt
     1021 tccatcggca ttgccctggc ggcttattct gagaaccagc ggcagagctt gcttacgtcc
     1081 ttttatgtca atctgcctgt aattcagctc tcgggagcaa tcgctcccct ggagagtatg
     1141 cccgagatct ttcaatggat gtccctcttg aatccgcttc gctactacgt tagctgcgtg
     1201 cggtccatta ttctcaaggg tgtcggtctg gaagagatct ggcctaatgt gctggctctg
     1261 gcggcatatg ccttgattct gctttccctc agctcttcaa gatttaggaa acaactgaaa
     1321 tgatgatgag atctaaattg aaactgacca ggctgcttct cttgctgagc tgctcacagt
     1381 ctttgtgttt tggcccgggt ccggcgcttg ccgcagctcc ggctgtttcc gatgggcagg
     1441 ctgatggccg tttgcaggct gatttgaatg cggcggagcc ggtgtccaca gacgaccttt
     1501 ccggggatgc cctcaatccc gagcgtattg atctaaagcg accatcctat aaggcgctta
     1561 tctccattga tcagaagatt gatccctttg tacttgatgc caccggctcc cggggtatcg
     1621 gcctggcgga tgtggtggat gccactctca agacgaatct ggatattggt atcgctgatt
     1681 acgacgagcg tatcaagcgc accaacttgc ttggctccta tggtaagttc ctgcccgatc
     1741 tgtctttgag ctatcaatat aactatctaa aaggcaaggc taatgtgcct ttcggggctt
     1801 cgctcgagcc tctggccttt aacaaccccc tgatcattac cagtgccggc tttaagtatt
     1861 atggttatcg gggcggcagc gttctcttcg gc
//
LOCUS       DAOQNZ010000877         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42546_length_1890_cov_3.213079, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000877 DAOQNZ010000000
VERSION     DAOQNZ010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42546_length_1890_cov_3.213079"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKW73_14375"
     CDS             <1..287
                     /locus_tag="PKW73_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74529.1"
                     /translation="LKASEKGASIVCGGIHMSDIPSFPYAALWQERSIKSVANLTRQD
                     AVEFLEIAGRIPVRAKVNLYPLNEANQALSDLKHGRFHGAAVLTMPDPSP"
     gene            complement(265..819)
                     /locus_tag="PKW73_14380"
     CDS             complement(265..819)
                     /locus_tag="PKW73_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74530.1"
                     /translation="MARKSLEFATVGESTASGPKISSRSPFAAGLSMWGGSFLMLLPG
                     GLIVTYIGEIIAGTTRHGVPSQVGLIVFLTGLVYLGFRLVSGQLKERRAVKELYEEQA
                     LLNRAKSKHGVLTVSEAALDCRLSILDAKQAFERLSKLSVCQIDVTEQGELCYRFPSF
                     ENPCEPVETLMPGFTLEVKEKDPA"
     gene            1044..1769
                     /locus_tag="PKW73_14385"
     CDS             1044..1769
                     /locus_tag="PKW73_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74531.1"
                     /translation="MLTRSRIKAVLKLSTGILSGAAASSSALLIIYMVVWYFQYRLPA
                     APPRLIDLRADSGAKPYYVCLCAALADNPHGFPGHSYVAFSRSLPFNLTEAESIGRVP
                     CRFQDQAWALFQPVPGMVMNRAAQGNLRNFNALVAVVDEDAYQRCLARVLLWQDRPFQ
                     VGKSDCVALTNSIAEVLNLKLPDQKIIYPQDYVKELKDLNCFSITDRKLQFTYGTLQA
                     EPLLNMSTIGSGKPFKRASRFSI"
BASE COUNT          485 a          531 c          452 g          422 t
ORIGIN      
        1 ctctaaaagc ttccgagaag ggagccagca tcgtctgtgg tggcattcac atgagcgata
       61 ttccctcttt tccctacgcc gctctctggc aagaacgctc cataaagtcg gtggccaacc
      121 tgacccggca agatgccgtg gaatttttgg aaatagcagg tcggataccg gtgcgcgcaa
      181 aagtcaacct ctatccactg aatgaagcca accaggcgtt aagcgatctc aagcatgggc
      241 gcttccacgg cgccgccgta ctgactatgc cggatccttc tccttaactt ccagtgtaaa
      301 accaggcatc aaggtctcaa caggctcgca agggttttca aaactgggaa agcgataaca
      361 gagttcaccc tgctcagtga cgtcgatctg acacaccgat agcttcgaga gtctttcaaa
      421 agcctgtttg gcatcaagaa tgctgagccg acaatccagg gcggcctcgg ataccgtcaa
      481 aacaccgtgc ttactcttgg cgcgatttag caaagcctgt tcttcataca attctttcac
      541 ggcccggcgt tccttcaatt gcccggaaac cagccgaaag ccaaggtaga caagaccggt
      601 caaaaataca atcaagccaa cttgcgaagg cacgccgtgc ctggtagtac cggcaattat
      661 ttcgccgata tacgtaacga tcaaaccacc cggtaaaagc atgagaaaac tgccgcccca
      721 catggaaagc ccggcagcaa aaggagagcg ggaagagatt ttaggcccgg aggcggtgct
      781 ttcacccaca gttgcaaatt caagagattt tcttgccatt ttaattgagc ccctgactgt
      841 actgtgcgcc aaacgagagg aaattatagc aatacctctg cgccccatgg tcgtcaacgc
      901 aaaggcgcca gaccggaacg ccattaatct accttgaagc ataagcaaac aattgagtaa
      961 ctgttcgggc ccgcctcaaa cagagccgcc gattcactat gcttgtctaa gcatttcacc
     1021 acaatgacca ggcatggaag gcaatgttga ctcgtagcag gatcaaagca gttctcaaac
     1081 tctcaaccgg catattgagc ggagcagccg cctcaagttc agcgctgctg atcatttata
     1141 tggtggtctg gtattttcaa tatcggctgc cggcggctcc gcccaggctc attgatctgc
     1201 gcgccgatag cggggcaaag ccttactacg tatgcctctg cgccgccctt gccgacaatc
     1261 cccacggctt tcccggccac agctatgtgg cgttcagtag atctctgccg ttcaacctga
     1321 ctgaagccga atccatagga cgagtgccct gccgctttca ggatcaagcc tgggccctct
     1381 ttcaaccggt accgggcatg gtaatgaaca gggcagcgca aggcaatctg cgcaatttca
     1441 acgcccttgt ggcggtagtg gatgaagacg cttatcagcg ttgcctggcc agagtgttgc
     1501 tctggcaaga ccggcctttt caggtgggca agagcgactg cgtcgctctt acgaattcca
     1561 ttgccgaggt actcaatcta aaactccccg accagaagat tatctatccc caggactatg
     1621 tgaaagaact gaaagacctg aattgtttca gcatcacaga tcgcaaacta cagttcactt
     1681 atggcacact ccaagccgag ccgcttttaa atatgagcac catcggctcc ggcaagcctt
     1741 tcaagagagc aagccgcttt tcaatctgag caattcccgg acaaacctta atctttaagg
     1801 ggaagccgca cttttacctg gcagttgtga catacttgcc ttgaatcagt caggctgtct
     1861 tgctcaccct ttgagtttta ttgccaaagg
//
LOCUS       DAOQNZ010000878         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42582_length_1889_cov_4.277535, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000878 DAOQNZ010000000
VERSION     DAOQNZ010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42582_length_1889_cov_4.277535"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="PKW73_14390"
     CDS             complement(<1..1480)
                     /locus_tag="PKW73_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNA74532.1"
                     /translation="MTLPVLIAGAGPTGLTLACDLLSRGIEFRIIDKAPAATAFSKAI
                     VLHARTLELLENMGLVEPFLEHGLPIRGSNFYAGGKRIVHLNMDEIESHYKYALSIPQ
                     SKTEEILNRKLEAGGCKIERNLELVELVEKGDLVEARLRRKNDDGSDTGAEEVLQCRY
                     LVGADGAHSTVRKQLGIAFEGAEYEEVFGAADVACDIQGDEMHGYLAEEGAVVFFPFG
                     EGRFRIIFSTGDGTADSAHFDTEQPLTMETVESVVKVRGPKNVKIDDPHWLSWFRIHK
                     RFAASYRRGRVFICGDAAHIHSPVGGVGMNTGMQDAINLGWKLALSIKGLSEEDLLDT
                     YEEERRKVGQSVLKGTHMATKFVTLRHPVALNIRNSLMHMLASHELVQQRILRTGSLT
                     GVSYRSSSLSCECHPPLDDSLGRTLRLSEDDGEEKPGLGAWIEFARAPVAGDRALDGE
                     CRDEQGNDRRLYQYLNSERFQLLLFDGFVASSDGYSGFTKIES"
BASE COUNT          461 a          537 c          493 g          398 t
ORIGIN      
        1 ggctttcaat tttggtaaag ccggaatacc cgtcggaaga ggccacaaat ccgtcaaaga
       61 gtaataactg aaaacgctct gaattaagat actgatagag tctgcgatcg ttgccctgtt
      121 catcgcgaca ctcaccgtcc agggctcggt cgccggccac aggtgcccgg gcaaattcaa
      181 tccaggcgcc aagcccgggt ttctcttccc cgtcgtcctc ggaaagtcgc agagtgcgac
      241 cgagggaatc gtcgagggga ggatgacact cacaggaaag tgaactggag cggtaagaca
      301 ccccggtcaa actaccggtg cgcaaaattc gctgctgtac aagctcatgg gatgccagca
      361 tgtgcatgag cgaattgcgg atattaaggg ccaccggatg tctcaaggtg acgaatttgg
      421 ttgccatatg ggtgcccttc aataccgact gcccgacttt gcgccgctcc tcttcgtaag
      481 tatcaagcag gtcctcttcg gagagccctt tgatggaaag agccagcttc catcccaggt
      541 tgatggcgtc ctgcatgccg gtgttcatgc ctacgccgcc gaccggactg tgaatgtgag
      601 cagcatcccc gcagataaac acccgccccc gccgatagga ggcggcaaaa cgtttgtgaa
      661 tgcggaacca actgagccag tgaggatcat cgattttgac gttcttggga ccacgaacct
      721 tcacaacgga ctctaccgtt tccattgtga gaggctgctc ggtgtcgaaa tgcgcactgt
      781 cagcggtacc atcgcccgtg ctgaatatga tccgaaatcg tccttcacca aagggaaaga
      841 aaaccacggc accttcctct gccaggtaac catgcatttc atcgccctgg atgtcgcagg
      901 ccacatcggc ggcaccgaaa acctcttcgt actcggcccc ctcaaaggca atgcccaact
      961 gcttacgcac tgtcgaatgg gcgccgtcgg cgccgaccag atagcggcac tgcaggactt
     1021 cctccgcacc agtatcacta ccgtcatcat tcttgcggcg cagcctggct tccaccaggt
     1081 cacccttctc aaccaactct accagctcca gattgcgctc aattttgcag ccccccgcct
     1141 ccagtttgcg attgaggatc tcttcagtct tactctgcgg gatagacagg gcgtatttgt
     1201 aatgggactc aatttcatcc atattgaggt gcacgatgcg cttgccgccg gcatagaaat
     1261 tgctgccacg aatgggcaga ccatgctcaa gaaaaggttc gaccagaccc atattctcga
     1321 gaagttcgag agtacgggca tgaagcacaa tcgccttgga aaatgccgtg gctgcaggag
     1381 ccttgtctat aatgcgaaac tcaatgccgc ggctcaagag gtcgcatgcc agggtcagac
     1441 cggtaggacc ggcaccggca atcaaaactg gcagtgtcat aatgcctccc taattccttg
     1501 gaaccgacga atttgaaaca agagcggtgg ctccgcttaa cctgattcgg caattataga
     1561 ctcttgattg cacggccgtt cataatcccc actaactccc aagattgagg cccgaaggat
     1621 ggtttttccg gcagatcagt caggaccatc cttattgctt attgccagat gaaatgccat
     1681 aacttaggat agggttaggc ctgccggggc agagcaggca ctggttcaaa ctctttactc
     1741 agcacttatt aaaacaagaa ggtagccaat gacagccagg gaaggaacgc ataaacccat
     1801 gcgcgcgctt gtcttgccgg gcggcggcgg aagaggcgcc taccaggtgg gagtaataaa
     1861 ggccctcaag caagcaggcc tcgaattcg
//
LOCUS       DAOQNZ010000879         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42592_length_1889_cov_3.450927, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000879 DAOQNZ010000000
VERSION     DAOQNZ010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42592_length_1889_cov_3.450927"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKW73_14395"
     CDS             <1..322
                     /locus_tag="PKW73_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA74533.1"
                     /translation="LSVTVLRDYTGADLPELVKKELKEWFGPECDKYEHLRTFRIERA
                     LPDQAPPWLDNPRRSVSVDDGSKTVYVCGDHRDNASINGAMVSGRRTAEQILKDLKVP
                     ISKA"
     gene            367..>1889
                     /locus_tag="PKW73_14400"
     CDS             367..>1889
                     /locus_tag="PKW73_14400"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74534.1"
                     /translation="MTFEDPTQQKKKVCTGCKREFPVTQTVCPYDKNMLMFMQKDELL
                     GTILNDRYRVLEEVGRGGMSIVYRGLHEMMDRTVAIKMLQAQHVTDQLSIKRFQQEAQ
                     AASHLQHPHVITVYDCGVVATGQPYIVMDFLEGESLSDVIKKENHIPFQRAVPIFAQA
                     CDALDHAHQKGVIHRDLKSSNIMLVEVEGRKDFVKVVDFGIAKLTNASGKMQQNLTQT
                     GEIFGSPIYMSPEQCLGQTLDARSDIYSMGAVLYESLTGLPPLMGDTIYATMKMHVSE
                     MPERFAQARPDLRIPEQIEQIAFKALAKKPEQRFQSMQEFRDALEQCMRTQFDTGAMP
                     SLLSAPPAVGFNTIAPGSRDNEDFDLSGGLFGDDPPPIVDKPSFRRTETGAGTVSPFV
                     TGPEGGTSGRITARTTRPPSVGSETGRRTAGVRKPMASDKKSGKAAKSFTMPAWVTPR
                     NLAIAAAALLVPALIAGVVVLLNQLGNDNPLLFTKKYDGTLFYYAPPSQTADGKEFPG
                     "
BASE COUNT          466 a          542 c          500 g          381 t
ORIGIN      
        1 gctatccgtt accgttttgc gcgactacac cggtgccgac ctgccggaac tggtcaagaa
       61 ggagctaaaa gagtggtttg gtcccgaatg cgataaatat gaacatttga ggaccttccg
      121 gattgaacgg gcgctgccag atcaagcgcc gccatggctg gacaatcccc gccgcagcgt
      181 cagcgtcgat gacggcagta aaaccgtata tgtttgcgga gaccaccggg ataacgcttc
      241 tataaacggc gccatggtct cgggtcgccg gaccgccgag cagattctca aggatctaaa
      301 agttcccatc agtaaggctt aagcttttgc taaaatcgaa gctgtaatca gtcgcataga
      361 tgacccatga ctttcgaaga cccaacccag caaaaaaaga aagtctgcac aggctgcaaa
      421 cgagagtttc ctgttaccca aactgtctgt ccctatgaca agaacatgct tatgttcatg
      481 cagaaggacg aactgctggg cacaatcctg aacgaccgtt atcgcgtcct ggaagaagtg
      541 ggtcgcggcg gcatgagcat cgtctatcgt ggtctgcacg aaatgatgga ccgcaccgtg
      601 gccatcaaga tgttgcaagc gcagcacgtc accgaccagt tgagtatcaa acgttttcaa
      661 caggaagccc aggccgcttc gcacctgcaa cacccacatg tgatcactgt ctacgactgc
      721 ggtgtcgtag ccacaggaca accttatata gtgatggact ttctggaagg ggaaagtctc
      781 tctgatgtaa tcaagaaaga gaaccatatt ccctttcaga gagcggtgcc gattttcgct
      841 caggcctgcg acgctctcga ccatgctcac cagaaaggcg taatccacag agatctgaag
      901 tcgagcaata tcatgctcgt agaagtggaa ggacgcaagg acttcgtcaa agtagtggac
      961 ttcggcatcg ccaagttgac caatgcctcc ggcaagatgc aacagaacct gacgcaaacg
     1021 ggagagattt tcggcagtcc tatatatatg agtccggagc agtgcctggg gcaaaccctt
     1081 gatgcgcgct cggacatata ttcaatgggg gccgtcttat atgaatccct gactggtctg
     1141 ccgcctctaa tgggcgacac tatatatgcc accatgaaga tgcatgtgtc tgaaatgccg
     1201 gagcgctttg ctcaggccag accagatctg agaattccag agcaaattga gcagatcgcc
     1261 tttaaggctc tggccaagaa gcctgaacaa cgatttcaaa gtatgcagga gtttcgcgac
     1321 gcgctcgagc agtgcatgcg cacccagttc gataccggcg ccatgccctc cctgctcagt
     1381 gcaccgccgg cagtcggctt caacaccatc gctccgggca gtcgcgataa cgaagatttc
     1441 gatctgtccg ggggactctt tggagacgac ccgccgccca tagtggataa gccttccttt
     1501 cgccgcaccg aaaccggtgc cggaaccgtc agccccttcg tcaccgggcc ggagggcggc
     1561 acatcgggac gaattacggc cagaaccacc cgcccgccca gcgtcggctc cgaaacaggc
     1621 aggcgcacgg ccggagttcg caaacccatg gcttcggaca agaagtcggg caaggccgct
     1681 aagtctttta ccatgcccgc ctgggttacg ccgcgcaatc tggccatagc cgcggccgcc
     1741 ctcctggttc cagcgctcat cgccggtgtg gtggtgctct tgaatcaact gggcaacgac
     1801 aatcccctcc tttttactaa gaaatacgac ggcaccctgt tttactacgc gccgccttca
     1861 cagaccgccg acggcaaaga atttccagg
//
LOCUS       DAOQNZ010000880         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42599_length_1889_cov_3.226827, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000880 DAOQNZ010000000
VERSION     DAOQNZ010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42599_length_1889_cov_3.226827"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..305
                     /locus_tag="PKW73_14405"
     tRNA            233..305
                     /locus_tag="PKW73_14405"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:266..268,aa:Phe,seq:gaa)
     gene            404..>1889
                     /locus_tag="PKW73_14410"
     CDS             404..>1889
                     /locus_tag="PKW73_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA74535.1"
                     /translation="MENSNRSKDTNSRLLLSGLLCLLFSLFFASGCAANPDFDALSDG
                     AKSGQPFLVKPYLQLGHEAGGYTICWFCREKSDIKVSYKTTGEKKFRPAESLSIREIT
                     LPTLKPCFRYSARLNQLKDGEGFSYVIEKEGKPLFQAEGKVYRAQAAAPGETKAKSKK
                     SGSGKIQGEKAITKIAVFGDCGAGSEGQRQVAKACQKENPDLVVIPGDVTYQRGLFSE
                     YLTNFFPYYNSDEEKQGASMMRSIPFVPVLGNHDIALSGRGTDLDRFPDALAYFIFWQ
                     VPLNGFDSTTGSSNTPALKGDINRQKLFTKSAQDAFPVMANFSFNFGNSHWTVLDGNY
                     YMNWSDEKIRAWLKNDLKSVKSGMWKFVTFHQPAFSIDAPHGKEQRMRLITDILQDYG
                     VDLVLCGHSHCYERTRPLTFAPLPGKTQLTMNADGTVDGSMKLDMDFDGAHTTTPKGL
                     IHIVTGAGGANLYKKDMSYHPAAGNDYMLHFMADKHSFTSLEIGE"
BASE COUNT          479 a          475 c          499 g          436 t
ORIGIN      
        1 gggtgaattt cccacagagg cagtggcgcg ctccggccgg gcatgggttc ttctgacaag
       61 ttatttgaaa cgaaaatatg caaattgagc aacttgagta agcgcaaaaa cctcctgtgg
      121 tatattgaac aagctaaagg gccggcgccc ttaagtaatg atttggggca tcttcgaggc
      181 tccagtcagg gttgctgttt aagcaaacta acagtgacca agcaagcaaa atgccgaggt
      241 agctcagtcg gtagagcaga ggactgaaaa tccttgtgtc ggcggttcaa ttccgcctct
      301 cggcaaattt ttaaactcac ccccgaagcc gttgtcaaag ctgccgttgt caaagctgcc
      361 gttgtcaaag ccgccgttat aaaagctgtc tttatcaatg ctgatggaaa attcaaaccg
      421 ctcaaaagac acgaacagcc gtcttttgct ttcgggtttg ctttgcctct tgttcagcct
      481 cttctttgcc agcggttgtg cagccaaccc tgattttgac gcgctgtcgg acggggcaaa
      541 atcagggcag ccttttctgg tcaaacccta tttgcaactc ggtcatgaag cgggtggcta
      601 caccatttgt tggttctgcc gggagaagag cgacatcaag gtgagctata aaactaccgg
      661 tgagaagaaa ttcagaccgg cggaaagtct cagtatcagg gagatcaccc tgccgacctt
      721 gaaaccttgt tttcgctata gtgctcgcct caatcaactg aaggatggcg aaggtttctc
      781 ttatgtgatt gagaaagagg gcaagccact ttttcaggcc gagggtaaag tttatcgagc
      841 ccaagccgcg gcgccagggg agacgaaagc caagagcaag aaatccggca gcggcaaaat
      901 tcagggcgaa aaggcaatta ccaagattgc tgtttttggt gattgcggcg ctggttcgga
      961 aggacaacgc caggtggcga aagcctgcca gaaggagaac ccggatctgg tggtgatacc
     1021 cggagatgtc acctatcagc gtgggctgtt cagcgaatac ctgaccaatt tcttccctta
     1081 ttacaacagc gatgaagaga agcaaggcgc ttccatgatg cgctcaatac cctttgtgcc
     1141 ggtcctgggt aatcatgaca tagccctttc cgggcggggc accgatcttg atcgcttccc
     1201 cgacgccctg gcttatttca ttttctggca ggtacctctc aatggtttcg acagcactac
     1261 cggtagtagc aacaccccgg ccttgaaagg ggacatcaac cgccagaaat tgtttaccaa
     1321 atctgcccag gatgcctttc cggtcatggc aaacttctct tttaattttg gcaacagtca
     1381 ctggacagtg ctggacggca actattacat gaactggtcg gatgagaaga tccgcgcctg
     1441 gttgaagaac gacttgaagt cggtgaagtc aggcatgtgg aagttcgtca ccttccacca
     1501 gccggctttc agcatcgatg cgccccatgg taaggagcaa cgcatgcggc taataacgga
     1561 catcttgcag gactatggcg tggatctggt gctgtgcgga cactcccatt gctatgagcg
     1621 cactcgtcca ctcaccttcg caccgctgcc cggaaaaact cagttgacca tgaatgcgga
     1681 cggtacagta gatggttcaa tgaagctgga catggatttc gacggggccc acacaaccac
     1741 cccgaaaggt ctgattcata ttgtcacagg agcgggggga gccaatctct acaaaaagga
     1801 catgagttat catcctgcag ccggcaacga ctacatgttg cactttatgg cggacaagca
     1861 ttccttcacc agcctggaaa taggtgaac
//
LOCUS       DAOQNZ010000881         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42741_length_1886_cov_3.914255, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000881 DAOQNZ010000000
VERSION     DAOQNZ010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42741_length_1886_cov_3.914255"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PKW73_14415"
     CDS             complement(<1..1304)
                     /locus_tag="PKW73_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNA74536.1"
                     /translation="MARVREQQQIRDRGVIKRHPRSPLWRLRIWQILLSLGALSIIGR
                     LYYLQIYQGPTLTEKAKTQRQQHHMLVHRGAITDRRGLPLAVDTTRYDVFVHVKLLKK
                     EKEEAARILSPIVKEPESKILKLFNSGYPIITLARHLDRECIDQLQELNWTGVDIVSR
                     PFRQYPEGKLAATLLGYTDMDTKGQGGVEQALQETLTDTGNIPKPQLDGHGRTILVPS
                     KQPSWDITPPLGRHVELTLDNYLQHLAEKELYAMCRHSHALRGSVILADPTNGEILAW
                     ANYPTFDPNNYTKFKYEHMKNWSMVDVYQPGSTFKIVTVASGLETGAIKPNQTFYDGG
                     SLTVGNRTIHNHDGGHGTINLKGLFIHSSNIASAMIGMAMTPKQFYDKLSEFGLGKPT
                     GIDLPGESGGILLNYKRWTPIDQATTGFGQGAIAVTPLQLVAA"
     gene            complement(1407..>1886)
                     /locus_tag="PKW73_14420"
     CDS             complement(1407..>1886)
                     /locus_tag="PKW73_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74537.1"
                     /translation="PVAFDYSEPRSDSARVVKNSSVQAVAPGRQQQQEARPYLVPLES
                     PKKGRRVPVLVRINRTLQAGLIALCGLAIFAYGMNVNDSHNVGKLQEQVRRLSEQNSE
                     LSNRVLKEVSYQGISENVSKHKGLVVPEVVKICKEVPAPKLSMVKAAKHHLPLMSGY"
BASE COUNT          383 a          459 c          551 g          493 t
ORIGIN      
        1 gctgccacta actgtagtgg cgttacggcg atcgctccct gtccgaatcc ggtggtggcc
       61 tggtcgattg gagtccagcg cttgtagttt agtaggatac cgcccgattc accgggtaga
      121 tcaatgcccg tgggtttgcc cagtccgaat tcggaaagct tgtcgtagaa ctgtttgggc
      181 gtcatggcca taccgatcat ggccgaggca atgttggaag agtgaatgaa gagacccttg
      241 aggttgattg tgccgtgacc gccgtcgtgg ttgtggatgg tgcgattgcc cacggtcagg
      301 gacccaccgt cgtagaaggt ctgattcggc ttgatggcgc cggtttccag accggaggcc
      361 acggtaacga tcttgaaggt ggaaccgggc tgatagacat cgaccatgga ccagttcttc
      421 atgtgttcgt atttgaactt ggtgtaattg ttcgggtcaa aagtcggata gttggcccag
      481 gctagaattt ccccgttggt gggatcagcc agaatgacgg agccgcgcag ggcatgggag
      541 tggcggcaca tggcatagag ttctttctcg gccagatgtt gcagatagtt gtcgagagtc
      601 agctccacgt ggcgccccag gggcggagta atatcccagg agggttgctt gcttggtacc
      661 agaatggttc ggccatgacc gtccagctga ggcttgggaa tgttgccggt atccgtcagg
      721 gtttcttgca gggcttgttc cacaccgccc tgtccttttg tatccatatc ggtatagccc
      781 agaagggtag cggcaagctt accttccgga tactgcctga aggggcggga aacgatgtct
      841 acgccggtcc aattcagctc ctgtagctgg tctatgcact cccggtccaa gtggcgtgcc
      901 agggtgataa ttggatagcc ggagttgaat agcttgagaa ttttgctttc aggctctttc
      961 actatgggag aaagaatgcg ggccgcttct tctttctctt tcttgagcaa tttcacatgt
     1021 acgaagacat cgtaacgggt tgtgtccacc gccaaaggca gaccacggcg gtctgtgatg
     1081 gcgccccgat gaaccagcat gtggtgctgc tgccgctggg ttttagcctt ttccgtgagc
     1141 gtgggtccct ggtaaatttg caagtagtag agcctgccga taatggacag tgctcccagg
     1201 ctcaagagaa tttgccagat gcgcaggcgc cagaggggtg agcgtggatg ccgcttgatt
     1261 acaccgcggt cccttatctg ctgctgttcc cttactctcg ccacctttca ccctttggat
     1321 tttgaactta tctagaaaga tagtttaaat cactgccttt atcaaaatga ttcaagcagt
     1381 ttcatctata tattgaatct gattctttag tagccgctca tcaaaggcag gtgatgcttg
     1441 gctgctttta ccatgctcag cttgggcgcc ggcacttctt tgcagatttt gacaacttct
     1501 ggaactacca gacctttgtg cttggaaacg ttttcggaaa ttccctggta ggagacttcc
     1561 ttcaatacgc ggttggaaag ttcggaattc tgttcgctga ggcggcgcac ttgctcttgc
     1621 agcttgccca cattgtggga atcgttgacg ttcatgccgt aagcgaaaat ggccagaccg
     1681 caaagggcga tcagtccggc ttgcaaagtg cgattgatgc gtaccaggac aggtacacgc
     1741 cgtcccttct tagggctctc aaggggaacc aggtaaggtc tggcttcctg ctgctgttga
     1801 cgaccgggtg ccactgcttg cacggaggag ttcttgacta ctctggctga gtcacttctt
     1861 ggctcggaat aatcaaaagc gacggg
//
LOCUS       DAOQNZ010000882         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42752_length_1886_cov_3.145276, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000882 DAOQNZ010000000
VERSION     DAOQNZ010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42752_length_1886_cov_3.145276"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1281)
                     /gene="corA"
                     /locus_tag="PKW73_14425"
     CDS             complement(148..1281)
                     /gene="corA"
                     /locus_tag="PKW73_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNA74538.1"
                     /translation="MTIPYTVAGMDPEALTFDPEASQSEITLVCYDGEKLLEEHNVEM
                     TRVVSQLEKWPVVWVNVEGLGSSKKIKWLGDLFKIHPLALEDIVTVHQRAKYETFDDC
                     CFFIAHMMEAKGELMQEQVSLYFNKNVVLTFQEGPIDATKPVLERIRKAQGRLRSSGA
                     DYLAYAIIDSIIDLYFPVLEYFGEKLENLEDAILDHPDKKTVSSLHMIKRELLTMRRA
                     LFPLREALSSMMRSSPAFFTPDTLLHLRDSHDHVVQITDFIDTYRELCSDLMDVYLSS
                     MSNRLSEVMKVLTVITTICAPPTLVAGIYGMNFNPDSSPYNMPELNWYFGYPLALVLM
                     VALSAALFMFILKPLKQLEGSTIIPSNVPPPAQSDKANGNGAS"
     gene            1502..>1886
                     /locus_tag="PKW73_14430"
     CDS             1502..>1886
                     /locus_tag="PKW73_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74539.1"
                     /translation="MTTPLASVGFKVFSSEEAMQLASVVAQRGERVGTALGSYMVLKT
                     GTRSQLWAKLDEENRPYSICPHFEGDSRNAVLLAQRIKYNNKPLDGRYLAWMNPNGNT
                     PGFGPPGEYTIIFECPNFAYYNFVEI"
BASE COUNT          484 a          479 c          488 g          435 t
ORIGIN      
        1 tcagaatcgg catcagccag ggatggattg agcatgatga tggagaggac gttttcacct
       61 gtgccggaaa gatttcgcca gagcaaatag cgatagcgac cgcttttatc aaaaatagca
      121 ccatcgccgc aaggcgggga cggcaagcta gcttgcgcca ttgccattag ccttgtccga
      181 ctgagccggc ggcggaacat tcgacggaat aatggtggaa ccttccaatt gcttgagcgg
      241 cttcaagatg aacataaaga gtgctgccga caatgccacc atcaagacca gagccagagg
      301 atatccaaaa taccagttca gttcgggcat gttatacggc gaactatcgg gattgaagtt
      361 cataccatag ataccagcca ccaaagtcgg tggcgcacaa atagtggtaa tgacggtaag
      421 aactttcatg acttccgaca gccggttcga catgcttgat agatacacat ccatcagatc
      481 ggaacaaagt tcacggtaag tatcgataaa gtcggtaatc tgcacgacgt gatcatggct
      541 gtcgcgcaag tgcaagagcg tatcgggtgt aaagaaagca gggctggagc gcatcattga
      601 actcaaagcc tcgcgcaaag ggaagagggc ccggcgcatg gtaagcagtt ctctcttgat
      661 catgtgcaaa gaacttacgg tcttcttgtc agggtgatcg agaatggcat cctcaagatt
      721 ttcgagcttt tcaccaaaat attccagcac cggaaaatac aagtcgatga tactgtcaat
      781 gatggcatag gccagatagt cggcaccgga ggagcgcagc cgcccctgcg cctttctgat
      841 gcgctccaag acaggcttgg tggcatcaat ggggccttcc tgaaaggtca gtaccacatt
      901 cttattgaaa tagaggctga cttgctcctg cataagctcg cctttagctt ccatcatatg
      961 ggcgataaag aagcaacagt catcgaaggt ttcatacttg gcccgctggt gcacggtaac
     1021 aatgtcttcc agagccaggg ggtggatctt gaacaagtcg ccaagccact tgatcttctt
     1081 ggagctgccc agcccctcca cattcaccca gaccacgggc cacttttcaa gctgggatac
     1141 gaccctggtc atttccacat tgtgttcttc tagaagtttt tcgccgtcat aacagacaag
     1201 tgttatctca gactgggaag cctcagggtc gaaggtgagc gcctcggggt ccatccccgc
     1261 caccgtatat ggtatcgtca tcgtagtccg aatcgattta cgaattgtct tgcgatgagt
     1321 cactgggctc tccggtttac gattgcactg gtctggaagt aacgctgcgt taagattaat
     1381 atagaaaggg tccgagaagc aagtctatcg ccgggaagtt gggcaactta gtccataaat
     1441 gcggcagcaa atgtgctaat ctgcctgaga ccatgcttct gttttaatca ggaccgcgaa
     1501 catgaccacc ccacttgcca gtgtcggctt taaggttttt agctcggaag aagctatgca
     1561 actagcttcc gttgtggcgc agcggggcga acgtgtgggc accgctctgg gcagctatat
     1621 ggtgctcaag accggcacca ggtcacagct ctgggccaag ctcgacgaag agaaccgtcc
     1681 ctattccatc tgtcctcact tcgagggcga ttcgcgcaac gccgtgcttc tggctcagcg
     1741 catcaagtac aacaacaagc ctcttgacgg gcgctacctg gcctggatga atccgaacgg
     1801 caatacacct ggtttcggtc cccccgggga atatacaatc attttcgaat gtccaaattt
     1861 tgcttactac aactttgtgg aaatac
//
LOCUS       DAOQNZ010000883         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_42833_length_1884_cov_3.263532, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000883 DAOQNZ010000000
VERSION     DAOQNZ010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42833_length_1884_cov_3.263532"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1884)
                     /locus_tag="PKW73_14435"
     CDS             complement(<1..>1884)
                     /locus_tag="PKW73_14435"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNA74540.1"
                     /translation="GTLVELFLARLRSYCSPVVFALSATIGNADLALETLVPSGDRII
                     ITGKEKKVIEARSVLPPRLSNLPWSGHTGLTMVDEVAKLAGEASSTLIFTNTRSQTEQ
                     WYQELIEIEPDFAGRAALHHGSLDTDVRRFVEENLRSGRINLVVSTSSLDLGVDFSPV
                     ERVLQIGSPKSVARALQRAGRSGHRPGAVSTLYMVPCHALELVELAAARIALTSGHLE
                     ERYPVLMPIDVLCQHITTTCLAGPLYPQQFLKEMRSTRAFRHLSEDELNWSLTFVATG
                     GSALAAYPEFKRIKLKEGYYEVDCETAEGKQLALKHRLSIGTIVADQSMEVRFFRGGS
                     LGHVEESFISRLKVGDTFLFAGRYLELVEVNGMKAYVRLSSKRGGPVPRWMGGRLPMS
                     SQLSAHVRYLIDQASHGKFEEPEMTFIKPLLLLQSQRSLLPAQDEVLVEWHKSRDGYH
                     LFIYTFEGRMVNEGLAYLSAFKLSRLSPATFSIAVNDWGFEILSDRPFRFERGASEFF
                     MAFKQEELSDDILACLNAGEMARRQFREIARVGGLIFQGYPGAPRKVKQVQISSSLLY
                     DVFVDYDPGNLLVRQAHQEVLDRHLEVQRMKLALERIVSGKVVEVETPKASPFALPLM
                     VDRL"
BASE COUNT          482 a          490 c          480 g          432 t
ORIGIN      
        1 caaacgatca accatcagtg gtaaggcgaa agggctggcc ttaggcgttt ctacctctac
       61 cactttgccg ctcactatac gctcgagggc aagcttcatc ctttgcactt cgagatgcct
      121 atctagaact tcttgatgcg cttgtctgac caaaagatta cctgggtcat aatcgacaaa
      181 aacatcataa agcagggagg aagaaatttg aacttgcttg actttgcgag gagcgcctgg
      241 atacccctgg aatataagac cccctaccct ggcaatctct ctgaactggc ggcgcgccat
      301 ttcgccggca ttgaggcaag ctagtatgtc atccgataat tcttcttgct taaacgccat
      361 gaaaaattcg ctcgcccctc tctcaaagcg gaaggggcga tctgatagta tctcaaagcc
      421 ccaatcgtta acggctatag agaaagtagc aggcgatagt cttgataatt tgaaggcgct
      481 cagataggct aaaccttcat ttaccattct gccttcaaaa gtgtatataa agagatgata
      541 gccgtcccgt gacttgtgcc attccacaag cacttcgtct tgggcgggca gtaaggagcg
      601 ctgcgactgc aaaagtaaga ggggtttgat gaaggtcatc tcgggctctt caaatttgcc
      661 gtgcgaagcc tgatcaatca gataacgaac atgggcagat aactgactgc tcatgggtaa
      721 acgcccgccc atccagcgag gcaccggacc gccacgtttt gaagaaagcc gcacataagc
      781 cttcatgccg ttcacctcga ccaactccaa atagcgtccg gcgaaaagaa aagtatcgcc
      841 gactttcagg cgagatataa aagactcttc cacatgacca agggagccgc ctcgaaagaa
      901 tcgtacttcc atcgattgat ctgcaactat ggtgccaatc gacaggcggt gtttaagggc
      961 cagttgtttg ccctcggcag tttcgcaatc tacttcataa taaccttcct taagtttaat
     1021 gcgcttgaac tcgggataag cagctagcgc actaccaccg gtggcaacga aggttaggga
     1081 ccagttcaac tcgtcctcac taagatggcg gaaggctctt gtacttcgca tctcttttag
     1141 aaactgctgc ggatagaggg gaccagccag gcaagtggtg gtaatgtgtt gacacagaac
     1201 atcgatgggc atgagcacag gatagcgttc ttccaggtgt ccggaagtaa gggctatgcg
     1261 ggctgcggcc aactcgacaa gttcgagagc atggcagggc accatataga gcgtgctcac
     1321 cgcacccggt ctgtgaccgc tgcgcccggc tcgctgaagg gcgcgggcga cgcttttcgg
     1381 gctgccgatt tgtaggactc tttccaccgg tgagaagtct acgccaagat ctaaagacga
     1441 agtggaaacg actaaattaa tgcgcccact gcgtagattc tcttctacga agcgtcgaac
     1501 atcagtatca agtgagccgt gatgcagtgc cgcccgtccg gcaaagtcgg gctcgatttc
     1561 aatcaactcc tggtaccact gttcggtctg agaacgggtg ttagtgaaaa tcaaggtgga
     1621 actggcttca ccggcgagct tagctacttc atccaccatg gttaagccgg tatgcccgct
     1681 ccatggtaaa tttgaaagtc gcggcggcaa gactgagcgt gcctcaatca ccttcttttc
     1741 cttccctgtg ataattattc tgtcgccgct gggcacaaga gtctcaagcg ccaggtcggc
     1801 attacctatg gtggcagaga gcgcaaatac tacgggggag caatagctcc ttaatctagc
     1861 cagaaacagt tccaccaggg tgcc
//
LOCUS       DAOQNZ010000884         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43098_length_1878_cov_4.205705, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000884 DAOQNZ010000000
VERSION     DAOQNZ010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43098_length_1878_cov_4.205705"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PKW73_14440"
     CDS             <1..243
                     /locus_tag="PKW73_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74541.1"
                     /translation="EFGKVEIDFGDQKHQPKVTMAAAENPPPTVSGPGCEFVIRQVPS
                     PGFGNEHEVDVKPPKDGGSCVVVIPPRDNGPNLSDY"
     gene            331..1035
                     /locus_tag="PKW73_14445"
     CDS             331..1035
                     /locus_tag="PKW73_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74542.1"
                     /translation="MQKNLSLFIAVAMLFCTALSPEAQAKPGANGAAGKGKYASKNIQ
                     LDLRDWGRLNAYLREDAIVIDDPLRDYTICCKAPDWVVSIYSKKRKIVSRVPLEKYDG
                     GLAGRLTKMTGGGFQKGQEWQVIGKGKVLDYLCTVRGIKPDAKNPRDRSCWRHYAARE
                     LPVKPQILDFYYKSYGIPPMDGFCLKFVTGSDMPSTLIEAHSIKDLKSNSNPFAIPAG
                     LKKASLEEVMFVQQDF"
     gene            complement(1073..>1878)
                     /locus_tag="PKW73_14450"
     CDS             complement(1073..>1878)
                     /locus_tag="PKW73_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74543.1"
                     /translation="LNDPDPAIRVEALRKLQAEESEEQVRESAIAALLDRSGRVRLLA
                     RYILKDIDPFALYLDRLASTRVTDSVLPTLLAALLECGKALPQSVLSEKIEPLVLSGL
                     APVRAGAYAILLDETANPGPQVPGSVDKVNLFKDCLLDGSRHCHKVAKRLLSGKGRRQ
                     PGFVLADKDLWQVFCRAPQSPAAACAIAAIARSRRWQSLPYLVEARWLALKRGRNAVV
                     LATIDNALAAFHTEYAPTEEERRSCLAAMENFGSALTEPMRNRLLAGLG"
BASE COUNT          497 a          542 c          488 g          351 t
ORIGIN      
        1 gaattcggaa aggtagaaat cgactttggc gaccagaaac atcaacctaa agtgaccatg
       61 gcagcagccg aaaatccccc gccgacagtc tccggaccgg gctgcgagtt tgtcatcagg
      121 caagtaccgt ctccaggttt tggaaatgag cacgaagttg acgtcaaacc accgaaggac
      181 ggcggcagtt gtgtagttgt aattccgccc agggacaatg gaccaaacct cagcgactac
      241 taaacttact tggtttccgc tagaattaac catactggca tcatcgttaa ttctgagatc
      301 aatagtgaac caaggaacgg ctgaagaacc gtgcaaaaga acctctcttt gttcatcgct
      361 gttgccatgc ttttctgcac ggcactaagc cctgaggcac aagccaaacc aggcgctaat
      421 ggagccgccg gcaaaggcaa atatgcaagc aaaaacatcc aactcgacct gcgagactgg
      481 ggacgtctga acgcctacct gcgcgaggac gccatagtca tcgacgaccc gctcagagac
      541 tataccatct gctgcaaagc accagactgg gtggtttcca tctactccaa aaaacgaaaa
      601 atagtaagcc gcgtgcccct ggaaaaatac gacggcggtc tggccggcag gctcacgaag
      661 atgaccggcg gcggctttca aaaaggccag gagtggcaag ttataggcaa agggaaagtg
      721 ctcgactatc tctgcaccgt aagaggcatc aagcccgatg ccaaaaaccc ccgagatcgc
      781 tcttgctgga ggcactacgc agccagagaa ctgcccgtca aaccgcagat cttagacttc
      841 tactacaaaa gctacggcat accacccatg gacggcttct gcctgaagtt tgtcaccggc
      901 agcgacatgc cttcaaccct cattgaggcg cactctatca aagacctgaa aagcaatagc
      961 aatccttttg ccattccggc cgggctgaag aaagccagcc tggaagaagt aatgtttgta
     1021 caacaagatt tctagggcag cagagtttcc accaccgccg acaggtatcc actcaaccca
     1081 gcccggctag aaggcgattg cgcatgggtt ccgtgagagc actgccaaaa ttttccatag
     1141 ccgccagaca actgcgccgc tcttcctcag tgggtgcata ctcggtgtga aaagcagcaa
     1201 gggcattatc gattgtagcc agcaccaccg cattccggcc acgtttaaga gccaaccagc
     1261 gcgcctccac aaggtagggc aggctctgcc agcgacgaga gcgggcgatg gcggcaatag
     1321 cacaagctgc ggccggtgac tgcggcgccc gacaaaacac ctgccagaga tctttgtcgg
     1381 ccaggacaaa gcctggctgc ctccgccctt tgcccgagag cagccgtttg gcgaccttat
     1441 ggcagtggcg actgccgtca agaaggcagt ccttgaacaa attcaccttg tcgacggagc
     1501 ctggcacttg cgggccagga ttggcggtct catccagtaa tatggcataa gcaccggctc
     1561 tcaccggggc taacccgctc agaaccagag gttctatctt ctcagaaagc acggactgag
     1621 gcagtgcctt tccacattca agcaaagccg caagcagggt gggcaagaca gaatcggtaa
     1681 ccctggtgct cgccaggcga tccaggtaaa gagcaaaagg gtcaatgtct ttcaaaatat
     1741 agcgagccag aagccgcacc cgaccggagc gatcaagaag tgctgcgata gccgactccc
     1801 gcacttgctc ctcgctttcc tccgcctgca gtttgcgaag tgcctccact cttattgccg
     1861 ggtcggggtc atttagaa
//
LOCUS       DAOQNZ010000885         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43109_length_1878_cov_3.361492, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000885 DAOQNZ010000000
VERSION     DAOQNZ010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43109_length_1878_cov_3.361492"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /locus_tag="PKW73_14455"
     CDS             <1..1616
                     /locus_tag="PKW73_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74544.1"
                     /translation="LKQRLEENFKLSNLEKQKQASGEAGQEPQEAQPARAEGSEEGST
                     EVSTEGDQNWFYSLMVVFLSFIFIYSSLALGQTMYGTPDLLASALGFTATALTLKLRR
                     LSRSGRGGAMLTALLLAFVGALGYFCKAPMLSYSYLLLLALVPETLSQKQIRKPLLTA
                     FVLLTVLVTPYIALISQKAGHLTFSDVWKVGQSWNIFLKQPIIHGRSPEFVHPTRLLG
                     TKPEIYEFQGQFPGTTYAPWYAPSYWYEGVPLIIEWHEYIPRVIANALRYTLLFGGGL
                     LILFLTAVCSKTVPLSRHALPETLPLWLPAAVGLGFIMLGIDVTVNSDRYYTPYLVPV
                     YACIFLSCTKPPRLQRRKVWLAAAAPIVGFILIRLALVGGGYWPLLTNQKALGEGFQS
                     VELLIPGALSQLGLKPGDRIAQLGSCRYFFARLAKLEIVADITDIKAFWQLSREGRQA
                     IYRRLQELGVKALVQEPYLKEVPLNMKEEDLAGTTTARDEGWQTIDENGRLIAVPEPG
                     KRPSTFMVKLLKENNIESRSEGKSEDKTQ"
     gene            complement(1623..>1878)
                     /locus_tag="PKW73_14460"
     CDS             complement(1623..>1878)
                     /locus_tag="PKW73_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74545.1"
                     /translation="PNRWYPVEFHTVLPFMHWLPPNQFRSILKALKKDYFADEANLNL
                     LDEKEFFALAPQGMKVQEAHARLLGPVSNLVLYGEWQGQD"
BASE COUNT          463 a          539 c          475 g          401 t
ORIGIN      
        1 agctgaagca acgcctggag gagaatttca aattatccaa tctagaaaaa caaaaacaag
       61 ccagcggcga ggccggacag gagccgcaag aagcccaacc cgctagagcc gaaggcagcg
      121 aagaaggcag cacagaagtc agcacagaag gcgaccagaa ttggttctac agcttgatgg
      181 tcgtcttcct cagcttcatc tttatatact cgagcctggc cctggggcaa accatgtacg
      241 gcaccccaga ccttctggcc agtgccctcg gcttcacagc cacggcgctc accctcaaat
      301 tgagacgctt gagccgaagc ggtagaggcg gagccatgct taccgccctc ctgctggcct
      361 tcgtcggcgc cctcggttac ttctgcaaag cgcccatgct ttcctactct tacctcttgt
      421 tgctggcgct cgtaccggaa actctcagcc agaagcaaat acgcaaaccg ctccttaccg
      481 cctttgtgct cctgacagtt ttggtgacac cctacattgc attaatatcg caaaaggctg
      541 ggcaccttac tttcagcgat gtgtggaagg tcggtcaaag ctggaacatc ttcctcaagc
      601 agcccataat tcacggacgc tctcccgaat tcgtacatcc cacccgcctg ctcggcacca
      661 agccggaaat atacgaattc cagggacaat tcccgggcac cacatatgcg ccctggtatg
      721 caccgtctta ctggtacgag ggcgtgccgc tcatcatcga atggcatgaa tacataccaa
      781 gagtgattgc caatgccctg cggtacacac tgctctttgg cggcgggctg ctcatccttt
      841 tcctgaccgc tgtatgctca aaaaccgttc ctttgagccg acacgccctg ccagagacat
      901 taccgctctg gttgccggca gccgtcggtt tgggctttat catgttgggc atagacgtga
      961 ccgtcaattc cgatcgttac tacacaccgt atctggtgcc ggtctatgcc tgcatcttcc
     1021 tgtcctgcac taagccgcct cgtctccaga ggcgaaaagt ctggctggcg gcggctgcgc
     1081 ccatagtagg cttcatcttg atcaggctcg ctcttgtggg cggtggttat tggccccttt
     1141 tgacaaacca gaaagccctg ggagaaggat ttcagtctgt agaattgctc ataccagggg
     1201 cactgtctca gctcggtctg aaacccggtg atcgcattgc ccaattgggc agttgccgtt
     1261 acttctttgc ccgtctggca aaactggaaa tcgtagccga tatcaccgac atcaaagctt
     1321 tctggcaact atccagagaa ggtagacaag ccatttacag aaggctgcaa gagctagggg
     1381 taaaagccct ggtgcaagag ccctatctca aggaagtgcc tctcaatatg aaagaggaag
     1441 atctggccgg cacgactacc gccagagacg aaggctggca gaccattgac gaaaacggca
     1501 ggctcattgc agtaccggag ccaggcaagc ggccctccac cttcatggtg aaactattga
     1561 aagagaacaa tattgaaagc cggtctgaag gcaaatcgga agacaaaacc caatgatcgg
     1621 gctcagtcct gaccttgcca ttccccataa agcaccaaat tggaaacagg tcccagcaag
     1681 cgagcatgcg cttcctgtac cttcatgccc tggggcgcca gggcaaaaaa ttccttctcg
     1741 tcgagcaaat tgagattggc ctcatcggcg aaatagtctt tcttaagggc tttcaaaata
     1801 gagcgaaact gattgggcgg cagccagtgc atgaagggca acactgtatg aaactccacc
     1861 ggataccagc ggttgggg
//
LOCUS       DAOQNZ010000886         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43299_length_1874_cov_3.028037, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000886 DAOQNZ010000000
VERSION     DAOQNZ010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43299_length_1874_cov_3.028037"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKW73_14465"
     CDS             complement(<1..302)
                     /locus_tag="PKW73_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNA74546.1"
                     /translation="MKHAIILAGGAGERMRPVQEDRPKCMVSVMGRPLLACMIQWLTA
                     YGIRNITLACGYKYELIQQYFGDGSSLGVTINYLIEDEPLGSGGALKNALRFLSPL"
     gene            600..>1874
                     /locus_tag="PKW73_14470"
     CDS             600..>1874
                     /locus_tag="PKW73_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74547.1"
                     /translation="MPPQNSRSDKEGALACLGATLVFACLLAYWWCLDHSFPLWDAGS
                     HFQDAIKYAQLLRHPHILQSSFWHEFLTVSFNYPLTQHLIFGASKALFGYGRFSDALV
                     NVLYLLLLSGSMARLIRLSGGSWRAASFAVILINCYPTVAQYSHSQMLDFGHIALSSF
                     GLCAMAQWWQKKSTANTIIMCIALSLAVTAKQAAMVFLLVPGLLVLVRSAAQKDKKAL
                     CQLFAAALSAALSLLIWLIPNRESLSAWRDYYTPQATQHAGPFLVFFEHLWLYLTSLP
                     SLMSPLLFLLWLISIGSLQRARTSLPRMLLLSAMVTGIPLMCLLSMNNAEARYIMPAL
                     LSPALETALLLAAWWQSGAYGKPRRLAALLTLVLALTQYLLLNFSPYPIKMPESLVNL
                     TKTLTRCETDLVGPGFAPVPAGDPWGQEWLIEH"
BASE COUNT          395 a          567 c          461 g          451 t
ORIGIN      
        1 tcaagaggcg agagaaaacg cagcgcattc ttgagcgcac cgcccgagcc caggggctca
       61 tcttctatca ggtaattgat ggtgacaccc agggaagagc catccccgaa atattgctga
      121 atcagttcgt atttgtagcc gcaagccagg gtgatattgc ggattccgta tgccgtaagc
      181 cattgaatca tacaggcaag gagcggacgt cccatgacgg agaccatgca cttaggtctg
      241 tcttcttgca ccggacgcat tctttcgccg gcaccaccgg caagaattat ggcgtgtttc
      301 atggaacatc tcccgagggg aactggtgat aaattgagcg atgcctggcc ttactttagc
      361 tagagggtca aatttactcg gcgattttac tttcaaattt gccccggccc gccggaaggc
      421 gaagcccgag caaaacgggg gtcgatatat gacgaatatt aaggcaggat acccccagcg
      481 cataggaaga attatttaag aggagaaata tcccctgtta aacccttcag caaatcgtca
      541 gggaaatgtt ctaagcttct tccatgcctt cagaatcgca acaagtagaa gcgagcttgg
      601 tgccgccgca gaattcaagg tccgataaag aaggggccct ggcctgcctc ggcgccaccc
      661 tggtctttgc ctgcttgctt gcctactggt ggtgcctgga ccacagcttt ccactctggg
      721 acgccggttc tcactttcag gacgccatca aatatgcaca gctactgcgc catccccaca
      781 tacttcagag cagtttctgg cacgaatttc tcacagtcag cttcaactac cctctcactc
      841 aacatctgat ctttggagcg tccaaagccc tcttcggcta cggtcgcttc tcggacgctc
      901 tcgtgaacgt gctttatctc ttgcttctat cgggcagtat ggctcgcctg atccgcctga
      961 gcgggggcag ttggagagca gcctctttcg ctgtgatttt aatcaactgt tatcccacgg
     1021 tggcacaata cagccactct caaatgctgg atttcggcca tatcgccctc tcttcctttg
     1081 gtctttgcgc catggcccag tggtggcaaa agaaaagcac cgccaacacc atcattatgt
     1141 gtatcgccct ttccctggca gtcaccgcca aacaggcagc catggttttc ttacttgtgc
     1201 cggggcttct ggtcctggtc aggagcgccg cccaaaagga taagaaagcc ctctgtcagc
     1261 tctttgccgc tgcgctcagc gcggcactct cgctgctaat ctggctcatc cccaatcgtg
     1321 aaagtctctc agcctggcgc gattactaca cgccgcaggc tacccagcat gccggacctt
     1381 tcctggtctt cttcgagcat ctctggctct atctaaccag cctgccgtcc ctcatgtcgc
     1441 cgcttctctt tcttctctgg cttatctcca taggctccct tcagagggcc cgcacctccc
     1501 tgcccagaat gcttttgcta agcgccatgg tcacgggcat tccgctcatg tgcctgcttt
     1561 ccatgaataa tgccgaagcg cgttatatca tgccggcgct cctgtcgccc gccctggaaa
     1621 cggctctcct tctggcggcc tggtggcaaa gcggcgccta cggcaaaccg cgcagactgg
     1681 ccgctctcct gactttagtt ctggccctga cacagtacct ccttttgaac ttcagccctt
     1741 atccgatcaa gatgccggaa agtttggtaa acctgacaaa aactctgact cgctgtgaga
     1801 cagacctggt gggtcccgga tttgcaccgg tgccggccgg cgacccctgg ggacaagaat
     1861 ggctaatcga gcac
//
LOCUS       DAOQNZ010000887         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43303_length_1874_cov_2.892798, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000887 DAOQNZ010000000
VERSION     DAOQNZ010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43303_length_1874_cov_2.892798"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1412)
                     /locus_tag="PKW73_14475"
     CDS             complement(81..1412)
                     /locus_tag="PKW73_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74548.1"
                     /translation="MSSPAKSPLKASNCPSKTVRRRQPEGAILPLAVFLMVVSIAFVA
                     LTVDVMRSAYTVQTLQHGTRACALAAFAGQVVSPALPYSQDQAKVNILNRLAEINNNS
                     WNSALKGPDASMEPRSAIRVEEADSEFLVNDQDPNEFFFHVKGKREGSDALKNMFMPL
                     IFAFNSLTGENVGETAPQIDVTVMGQPANRIGKGVSQLSSGSQARLAGFAALPLAISN
                     LQFASLANPASAPLSTYTIDVLRNSSDTLTPGHIKGGLVNLTAGTSGYYGDISGEKAV
                     TDNLANFKYFLASAPGAIPPLALERGAVLGVIRPQGQSWDNQLSNWQALAVSLKARPY
                     IYIFPVLKNDPLPGTTGNEVAGFAIFQLQDVIVNGSGTSGAALSIVVKPVQSMVLPNA
                     CLASRRSLQSQAAMPAPVYPFQARVRTAPASASPSPRCLLLAPAISQAQGG"
     gene            complement(1375..>1874)
                     /locus_tag="PKW73_14480"
     CDS             complement(1375..>1874)
                     /locus_tag="PKW73_14480"
                     /inference="COORDINATES: protein motif:HMM:NF028286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HNA74549.1"
                     /translation="SKDGKKYTRTICQNLPVIYSLPKEALKSKSLQGNDAARITLAVT
                     PEQAEILAQAEETGRIKLALRNRLSVTAPHSYGVSEEDLLPAKALTVSAAAPAPVQPA
                     TAPATANFEPIAPPPPPFIMPPTFNEVASRPEVKKAAQWVVEVFTGNRRETHEFPRQE
                     PTESK"
BASE COUNT          357 a          486 c          573 g          458 t
ORIGIN      
        1 cacctatagt gagagtgcct gcgctgcttt cagctgctct cggctgacgt ttttcatttt
       61 ccagtttggt caccatggtg ctagcctccc tgcgcctggc tgatagccgg tgccagaagc
      121 aggcagcgcg ggctggggct ggcggaagcg ggagcggttc tcactcttgc ctggaaggga
      181 taaaccgggg ccggcatggc ggcctgactt tgcagggagc gtctgcttgc caggcaggca
      241 ttgggcagga ccatcgattg aaccggcttg accactatgg aaagagcggc accactggtg
      301 ccggaaccgt tcacaatcac atcctgcaat tgaaagatgg caaatcccgc cacttcgttg
      361 ccggttgtac ccggcagggg atcgtttttc aaaacaggaa atatatagat gtaaggtctg
      421 gcttttaagc tgacggcaag agcctgccag ttgctcaatt ggttgtccca gctttgtccc
      481 tgcgggcgaa ttacgcctag aacggctcct ctttccagag ccagaggtgg tattgcccct
      541 ggtgcagaag ccaggaagta cttaaagttg gccagattgt cggtgacggc cttctcgccg
      601 cttatatcgc cataatagcc gctggtgccg gccgtcaaat tgaccagtcc tcctttgatg
      661 tgaccggggg tgagagtatc cgagctattg cgcaggacat caatggtata ggtgctcagg
      721 ggcgcgctgg cgggattggc cagggaggca aattgcaagt tgcttatggc cagaggcagg
      781 gcggcaaaac cggccagtct ggcctggctg ccgctgctta attggctcac tcccttaccg
      841 atgcggtttg ccggttgacc catcaccgtc acgtcaatct gcggggcggt ctcgccgacg
      901 ttttcgccgg tgagagaatt gaaggcgaaa atcaggggca tgaacatatt cttgagagca
      961 tcgctgcctt cccgctttcc ttttacatgg aagaagaact cgttcgggtc ctgatcgttc
     1021 accaggaatt cactgtctgc ctcttccact ctaatggcac tcctgggttc cattgaggcg
     1081 tccggacctt tgagggcgct gttccatgaa ttgttgttga tttcggctaa ccggttcaga
     1141 atattgacct tggcctgatc ctgggagtag ggaagagcag ggcttaccac ctgaccggca
     1201 aaagcagcca gtgcacaagc gcgagttccg tgttgcagag tttgcaccgt ataggctgag
     1261 cgcattacgt ctacggtcag ggccacaaag gctatactca caaccatcaa aaaaacggca
     1321 agcggcaaaa tcgcgccttc aggttggcgc cgccgcacgg tttttgatgg gcaattactt
     1381 gctttcagtg ggctcttggc gggggaactc atgggtctct cttcggtttc ctgtgaatac
     1441 ttcaactacc cattgggctg ctttttttac ttccgggcgg ctcgctactt cattgaaagt
     1501 gggaggcatt atgaacggtg gcggcggtgg tgctatgggt tcgaaattag cggtggcggg
     1561 tgcagtcgcc ggttgcacgg gcgccggggc ggcggcgctc acggtcaggg ccttggccgg
     1621 caggaggtct tcctccgaaa caccgtaact gtgcggggct gtaacgctga gacgattgcg
     1681 cagagccagt tttatgcgac cggtttcctc ggcctgggcc agtatttctg cttgttccgg
     1741 cgtgacggcc agggtgatac gggcagcgtc attgccctgc aagctcttgc ttttgagggc
     1801 ttccttgggc aggctataga tgacaggcag attctggcag atagttctgg tgtacttttt
     1861 gccgtctttg ctgg
//
LOCUS       DAOQNZ010000888         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43368_length_1872_cov_4.373143, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000888 DAOQNZ010000000
VERSION     DAOQNZ010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43368_length_1872_cov_4.373143"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PKW73_14485"
     CDS             complement(<1..1071)
                     /locus_tag="PKW73_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA74550.1"
                     /translation="MPSLDETIETLRAALKLSPDNLPLRMLLGENLLASGRLEEAERE
                     FTVALALAPSDLKLAVSLAQACFGNGKYSQALVIIEDVIRKSDVPPKAFLLYARLLLN
                     EGKLASAQENYNQAKGLDPSLRDATLEERLGLKGDSGQSSLKENREQRPMGATASGEM
                     REIEREKDSAYGSDNYLASEFDDLVETADTGFADVGGMDAVKEQIRMKIIYPLKHPET
                     FKAYGKKMGGGILLYGPPGCGKTYLARATAGEIEARFIPIGLHQVLDMWIGSSEKNLH
                     EIFEIARKQKPSVLFFDEVDALAASRADMKQTYHRHTINQFLNELDGVNTSNEGVLIL
                     AATNAPWHLDSAFRRPGRFDRII"
     gene            complement(1128..1580)
                     /gene="xseB"
                     /locus_tag="PKW73_14490"
     CDS             complement(1128..1580)
                     /gene="xseB"
                     /locus_tag="PKW73_14490"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNA74551.1"
                     /translation="MTNEFKQLSLLGEITGEPSDQNSEQKPENKAKKVKKPGKEKPRE
                     SDQDGSYDIQTKEPKKELTRDEFMTTANEEKIDFEASLLELETVVKQLDSEVKLDKAL
                     KLFETGIKLSVDCEQFIKSAKRKIEILKKKADGTMELSDFSESDDSAQ"
     gene            complement(1570..>1872)
                     /locus_tag="PKW73_14495"
     CDS             complement(1570..>1872)
                     /locus_tag="PKW73_14495"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNA74552.1"
                     /translation="EGRLDLSLANVQRNKQILTGSIDRLVAKQEHQFLKLRERLKDLG
                     PGSVLSRGFSILRSLEGNVISEAGDLKAGDKVEAIFKVGRVKMTVDSVDRTKCYDQ"
BASE COUNT          369 a          482 c          478 g          543 t
ORIGIN      
        1 gatgatgcga tcaaagcgtc ccggtcttcg aaaggcggaa tccagatgcc agggcgcatt
       61 ggtggctgcc agaatgagca ccccttcgtt ggatgtattg acgccgtcaa gctcattgag
      121 gaactgattg atggtatgtc tgtggtaggt ctgtttcata tcggcgcggc ttgccgccag
      181 ggcatcgacc tcgtcaaaga aaagaacact cggcttttgc ttcctggcga tttcgaagat
      241 ctcatgcaga ttcttttcac tgctgcctat ccacatgtcc agtacttgat gcaagccgat
      301 gggaataaag cgggcctcta tttctccggc agtggctctg gctaaataag tcttgccgca
      361 accaggggga ccgtagagca aaattccacc ccccattttc ttgccgtagg ctttgaacgt
      421 ctccgggtgt ttgaggggat agataatctt catgcgaatc tgttctttga cggcatccat
      481 gccgccgaca tcggcgaagc cggtgtcggc tgtttctacc agatcgtcga attcgctggc
      541 gagatagttg tctgacccgt atgctgaatc cttctccctt tcaatttctc gcatctcgcc
      601 ggaggcggtg gcccccatcg gtcgttgttc gcggttttct tttagagacg actgaccgga
      661 gtcgcctttc agaccgagcc gctcttccaa cgtggcatcg cgcagagatg gatccagccc
      721 ttttgcctgg ttgtaatttt cttgtgccga ggccagctta ccttcattca aaagcagtct
      781 ggcgtagagc aagaaggctt taggcggcac atcgctcttg cggatgacat cttcgattat
      841 gaccagcgcc tggctgtatt ttccgttacc aaagcaggcc tgggccaggc tcactgccag
      901 ttttagatcg cttggtgcaa gggccagggc gacagtgaat tcacgctctg cttcttccag
      961 tctgccggaa gcgagcaggt tttctcccag gagcatgcgc aggggcagat tatcggggga
     1021 gagcttgagg gcggctctca gggtttcaat tgtttcgtcc aggctgggca ttagaatctc
     1081 cggaaagaat atctccgaca aaagcaggtt gaaccaagat cgcctgtcta ttgagcgctg
     1141 tcatcacttt cgctaaagtc tgaaagctcc atggtgccat cggctttctt cttgagaatt
     1201 tcaattttgc gcttggcgct cttgatgaac tgctcgcaat cgacagatag ctttataccc
     1261 gtctcaaaga gctttagtgc tttgtctaat ttgacttcgc tatcaagttg cttgacgacg
     1321 gtttcaagct ccagaaggga agcttcaaag tcgattttct cttcatttgc agtagtcata
     1381 aactcatccc tcgtcagttc tttctttggt tccttggttt gaatgtcgta agacccgtct
     1441 tggtcgcttt ctctgggctt ttctttgcct ggtttcttta ctttctttgc cttgttctca
     1501 ggtttttgct ccgaattttg atcagaaggc tctcctgtaa tttcgccgag caacgaaagt
     1561 tgcttgaact cattggtcat agcactttgt cctgtcgacg ctgtctacgg tcattttgac
     1621 tctgccgact ttgaaaatgg cctctacttt gtcgccggct ttgaggtctc cggcttcact
     1681 aatgacgttg ccctcaaggc tgcgcaagat ggaaaaacca cggctgagca cgctgccagg
     1741 accaaggtct ttcagccttt cgcggagttt gaggaactga tgctcttgct ttgccacaag
     1801 gcgatctatg ctgcccgtca aaatttgctt gttgcgttgc acattggcca ggctgagatc
     1861 gagtcgccct tc
//
LOCUS       DAOQNZ010000889         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43429_length_1871_cov_3.577643, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000889 DAOQNZ010000000
VERSION     DAOQNZ010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43429_length_1871_cov_3.577643"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="PKW73_14500"
     CDS             complement(<1..1227)
                     /locus_tag="PKW73_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNA74553.1"
                     /translation="MSKDEQILTKGQGQPTPPKAPLKPAPKSHRQLSEGRWEPLSDQK
                     AGSPDGPEKPPSLFKFDTWLWYFFLSLWLAMMFSGFIGAAQTENIPYSAFMNYVQEGK
                     VDDVVVAQQDIRGTFKDEKAKVHKFRTVRIEDPQLASRLNDHKVKFQGELEDTLGKAL
                     LSWVVPLIILMGIWSLIASRMASKDGFGGMGPGMGLMSIEKSRAKVYMESNIKITFED
                     VAGVDEAKEELQEVIGFLKEPDRYNRLGGHLPKGILLVGPPGTGKTLLARAVAGEAGV
                     PFFSISGSEFVEMFVGVGAARVRDLFAQAQKSAPCIIFIDELDALGRARGLNPMGGGH
                     DEKEQTLNQLLVEMDGFDPRQGVVLLAATNRPEILDQALLRAGRFDRHVLVDRPDRVG
                     RAQILKVHMRNISCDES"
     gene            complement(1318..>1871)
                     /locus_tag="PKW73_14505"
     CDS             complement(1318..>1871)
                     /locus_tag="PKW73_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNA74554.1"
                     /translation="RQYHEPLLMYRKDFGGVLSPRNAWPILVYGLPTLAARMANQQKS
                     ALHVARYLEKHPKVARVMYPGLESFPQHELARRQMVNYEGKFAPGSMLYFVLKGDLKE
                     ASEAAERCIDYLAGNAYSITLAVSLGQIRTLVEEPFSMTHSALPPEVKIAKGLEPGGI
                     RLSIGLEDWHDITDDLERALAQT"
BASE COUNT          413 a          554 c          499 g          405 t
ORIGIN      
        1 actctcgtcg caggagatat tgcgcatgtg cactttcagt atctgggccc ggccgactcg
       61 atccggtcta tccaccagca catgccggtc gaagcggccg gctctcagta gcgcctggtc
      121 aagaatctcc gggcgattgg tggcagccag gagcaccact ccctggcggg ggtcgaaacc
      181 gtccatttcc accagcaact gattgagagt ttgctctttt tcatcatgac cgccgcccat
      241 gggattcaag ccccgggccc ggccaagggc atccagttcg tctatgaaaa ttatgcaggg
      301 cgcgctcttc tgcgcctggg cgaaaaggtc tctaacgcgg gcggcaccta caccgacaaa
      361 catctccaca aattccgatc cgctgatcga gaaaaacggc acaccggctt caccggccac
      421 ggcccgggcc aggagagtct taccggtgcc gggaggaccg accagaagta tgcctttggg
      481 cagatgcccg ccaaggcgat tatagcgatc cggttctttc aggaaaccga ttacttcctg
      541 caactcttct tttgcttcat cgacaccggc cacatcttca aaagtaattt tgatgttgct
      601 ttccatatag accttggccc ggctcttctc gatggacatc agacccatgc ccggtcccat
      661 accgccgaat ccgtcctttg aggccattct ggaagcaatg agcgaccaga tacccatcaa
      721 aataatcaga ggcaccaccc aggaaagaag cgctttcccg agagtatctt ccagttctcc
      781 ctgaaatttg accttatgat cgttcaggcg ggaagccaac tgcgggtctt caatgcgcac
      841 ggtgcgaaat ttgtggacct tcgctttctc gtccttgaaa gtaccgcgga tgtcctgctg
      901 agcaacgacc acgtcgtcca ctttgccttc ctgaacataa ttcatgaaag cgctgtaggg
      961 aatattttca gtctgagcgg cgccgatgaa accggagaac atcatggcca gccaaagtga
     1021 aagaaagaag taccagagcc aggtatcgaa tttgaagaga ctgggaggtt tctccgggcc
     1081 atcgggagag ccggctttct ggtcgctcaa aggttcccag cgcccttccg aaagctgtcg
     1141 atgactcttg ggggcgggct tcagcggcgc cttaggcgga gtcggctggc cctgaccttt
     1201 ggttagtatc tgttcgtctt tggacacact cacctcagcc cgctcttcta aaaccaattg
     1261 tgcaacctgg aataactgcg ctagacattc agtttaacac cagcttctgc cgggccccta
     1321 agtctgggcc agggcacgct ccagatcgtc ggtgatgtcg tgccagtctt ccaaaccgat
     1381 ggagaggcga atgccgccgg gctccagtcc cttggcaatc ttcacctccg gcggcagagc
     1441 cgaatgagtc atggaaaacg gctcctccac aagggtacga atctgcccca gactaacagc
     1501 cagggtgatg ctgtaagcat taccggcgag gtaatcgatg caacgctccg ccgcctcgct
     1561 ggcttccttg aggtcgcctt tcagcacaaa ataaagcatg gagcccggtg caaacttccc
     1621 ttcgtaattg accatctggc gcctggccag ttcatgctgc ggaaaacttt ccagacccgg
     1681 gtacatcacc cgggccactt ttggatgctt ctccagatag cgagccacat gcagggccga
     1741 cttctgctga ttggccatgc gagctgccag agtgggcagt ccgtagacga gaataggcca
     1801 ggcattgcgc ggtgacagga cgccgccgaa gtctttccgg tacatgagca gaggctcgtg
     1861 atactgcctg g
//
LOCUS       DAOQNZ010000890         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43692_length_1865_cov_5.157459, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000890 DAOQNZ010000000
VERSION     DAOQNZ010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43692_length_1865_cov_5.157459"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKW73_14510"
     CDS             complement(<1..209)
                     /locus_tag="PKW73_14510"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HNA74555.1"
                     /translation="MINIGSQLLEAAEELERERNIPQEEFIAYVCDAIVAAYKKKVPD
                     HDVEGVRAIFDTETGEIGIFAPKEVV"
     gene            complement(289..942)
                     /locus_tag="PKW73_14515"
     CDS             complement(289..942)
                     /locus_tag="PKW73_14515"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74556.1"
                     /translation="MPTSKNHGPEQALAIITQAAEEIAQSLGFQLVDAHFGQQGRKTS
                     LEITIYKPGGRIGLADCEKVSRELESKLESLTELPALLHGSYVLDVCSPGIERVLKHE
                     REYKIFAGSQVELKAKADVGAGGFGQHFLATLVGLDGDKVKLAALKELPRGNDKGSAG
                     KKGSGKNKKDKIEIEPVHELSVPLKQITALKLYADLSKAAHDNQECEALEAEASLES"
     gene            1216..1785
                     /gene="ybeY"
                     /locus_tag="PKW73_14520"
     CDS             1216..1785
                     /gene="ybeY"
                     /locus_tag="PKW73_14520"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNA74557.1"
                     /translation="MKIVFQNRQRRFSLDFEELQADYALLSRTLAESLLQSAPVKPYG
                     GKVLTASLVEDLFCESAVSVVFLSNRAIRKLNNDFRGIDKGTDVLSFPLDLERPATCL
                     SFPQEDDSDCAWLLGELFISIEKASMQADEYGHSLRREMAFLFVHGLLHLLGFDHESK
                     TQEKQMFAYQNKILEKVGINREGCQVKLP"
BASE COUNT          395 a          437 c          479 g          554 t
ORIGIN      
        1 actacttcct taggggcgaa aatgccgatt tcaccggttt cggtatcgaa aatggctctt
       61 acgccttcca catcatggtc cggtaccttt ttcttgtaag cggccacaat ggcatcgcaa
      121 acataggcaa tgaactcttc ctggggaata tttctttctc tttccagttc ctcggctgct
      181 tcgagtagtt ggctgcctat gtttatcact ttgtacctcc cggtatgggc tcgtttacta
      241 gggttctatc cgttggtttg ttagtggttg aaatgccgaa aatttggttc aactctccag
      301 tgaagcttct gcttccaggg cttcgcattc ttgattgtca tgggccgcct tactcaggtc
      361 ggcataaagt ttcagggcgg tgatctgctt gagcggaacg ctcaattcgt gcaccggctc
      421 aatctcgatc ttgtcttttt tgttcttgcc ggagcccttt ttaccggctg agcccttgtc
      481 gttgcccctg ggcaactctt tcaaggcggc cagtttgact ttgtctccgt cgagtcccac
      541 cagggtagcc aggaagtgtt gaccgaagcc gccggcgcct acatcagcct tagcttttag
      601 ttcgacctga ctgccggcaa aaattttgta ttcacgctcg tgttttaaga ctctttcaat
      661 gccgggactg catacatcca gaacataaga tccgtgcagg agggcgggca attcagtgag
      721 gctctccagt ttgctttcca gctcgcgact gaccttctcg cagtctgcaa gaccaatgcg
      781 tccgcccggt ttatagatag tgatttccag gctggttttg cgtccctgct ggccaaagtg
      841 cgcatcgacg agttgaaaac caaggctctg tgcgatttct tccgcagcct gggtgataat
      901 ggccagtgct tgttcgggtc cgtggttctt acttgtgggc attaaattga atttctactc
      961 tggttggtgt tgattggcga gcggcttgct tcgattgtcc tggtcggtgc gggggactgt
     1021 tcccgaacta tgaacggttt cctctatgtc aggttcaagt tccagtttgg cgttggaacg
     1081 taaacaagtg tcgcctgaga gcgcaattaa acacaaaaat gccgtaggct aaggtcttgc
     1141 ctacgactaa aaggtctcta agtgaaggga tgatagcacg aagcaagagg gatcaacagg
     1201 gggatggttt gacttgtgaa gattgttttt caaaatcggc aaaggcgctt ctctcttgat
     1261 tttgaggagc tgcaagccga ctatgcccta ctcagtcgca cccttgctga gtctctatta
     1321 cagtcggcgc cagtgaagcc ttatgggggc aaagtgctga ctgcttcctt agttgaagac
     1381 ttgttttgcg aaagtgcggt ttctgtggtg tttttgtcca atcgggccat tcgcaagctt
     1441 aataatgatt ttcgcggaat tgataaaggc accgatgtgc tctcttttcc gctggattta
     1501 gaaagacccg ccacctgcct gtctttccct caagaagatg attccgattg tgcctggctt
     1561 ctgggcgaat tgtttattag tatcgagaag gcttctatgc aagccgatga atacggacat
     1621 tccctcaggc gagaaatggc atttcttttt gtacacgggc tcttgcatct gcttggtttt
     1681 gaccatgagt cgaagaccca ggaaaagcag atgtttgctt atcaaaacaa aattttagag
     1741 aaggtaggta tcaatcgtga aggttgccaa gtaaaattgc cctgaaccgg tgttgttcgt
     1801 gtcatgattg acttggcgac cagatcttgc cgaaattcga tagatgccag accggtaacg
     1861 gttat
//
LOCUS       DAOQNZ010000891         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43712_length_1865_cov_3.056354, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000891 DAOQNZ010000000
VERSION     DAOQNZ010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43712_length_1865_cov_3.056354"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PKW73_14525"
     CDS             <1..523
                     /locus_tag="PKW73_14525"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HNA74558.1"
                     /translation="LTGQSFQPGITFDHTHDDLHFQSTFSECDLRHYLKNERKDTFDV
                     IYHDPFSPRKVTELWTLEVFAAYKQRLQPNGSILTYSAASAIRGALRQLGFHVYRTAA
                     VGGKSGGTLACLQDLQDYLPKLADVIMPLSEEEERRLRTTSGLPYRDPELSLTGEAIR
                     IARLKEQGQIKDG"
     gene            588..1013
                     /locus_tag="PKW73_14530"
     CDS             588..1013
                     /locus_tag="PKW73_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74559.1"
                     /translation="MHIRFAHLNNNTKLEGLASFVSYMQERKVENSDGAMIVERDARE
                     RIIASTSWDSLLGKAELQCFAGKSTWDLIDVLDCILSGEYELTGVKPLDDTYAILEFE
                     PLAIPFGGTDPLKALVEGFGFDVVGDSFQDGFVEWQKSE"
     gene            complement(1022..1378)
                     /locus_tag="PKW73_14535"
     CDS             complement(1022..1378)
                     /locus_tag="PKW73_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74560.1"
                     /translation="MADRPEQDKGADIPDQNWLKPDLSSVSQLLCTPQEMATILDDRP
                     GPAGGTFSESLSECANRIVSDQSKNELQKALAFKNLIVDTEKLEKKGQGGDIKLLTDA
                     NGNVTGFDISFPIQRK"
     gene            complement(1485..>1865)
                     /locus_tag="PKW73_14540"
     CDS             complement(1485..>1865)
                     /locus_tag="PKW73_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNA74561.1"
                     /translation="TVESIMRSGLDVPIVSLDASHEMVLEEITRKKLGFTVVCEDDGK
                     LRGIITDGDLRRALSKYRDGVFEKQARDMMTTSPKTISAHCLAVEALKLMERYSISDL
                     LIVDSAQRPVGLIDLKDLLRAGII"
BASE COUNT          442 a          491 c          472 g          460 t
ORIGIN      
        1 gctaacggga cagtcatttc agcctggtat cacattcgac cacacgcacg atgatttgca
       61 tttccaatcg actttcagtg aatgcgacct gcggcattac ctgaagaatg agcggaaaga
      121 tactttcgac gttatctatc acgatccctt ttctccccgc aaagtaaccg aactctggac
      181 cctggaagtg tttgccgctt acaagcaacg cttgcagcca aacggcagca tcctcactta
      241 ctcagctgct tctgccatac ggggagccct caggcaactg ggttttcatg tttaccgcac
      301 cgctgccgta ggcggcaaga gcggcggtac cctcgcctgc ctccaggatc tccaggacta
      361 tttgcctaag ctcgcagacg taatcatgcc cctgagcgag gaagaagaga ggcgactgcg
      421 caccacctcg ggtcttcctt accgtgatcc ggaactctca ttaacgggag aggcaattcg
      481 cattgccagg ctgaaggaac aagggcaaat caaggacggc tgatcaggga atagaaacct
      541 tgaaagagtg ctattcttga gctgtggctc tctatcggtg taggcaattg cacattcgtt
      601 tcgctcacct caataacaat actaaactcg aagggcttgc ctccttcgtc agttacatgc
      661 aagagcgcaa agttgaaaat tccgatggtg ccatgatcgt agagcgtgat gcaagagaac
      721 gtatcatcgc ctccaccagt tgggacagtt tgcttggcaa ggcagaattg cagtgctttg
      781 ccggcaagag cacctgggac ttaatagacg ttctcgattg catcttatct ggcgagtatg
      841 agctgaccgg tgtcaaaccg cttgatgaca cctacgccat cctggaattc gagccgctgg
      901 ctattccctt tggcggcacg gatccactca aggccctggt tgaaggtttc ggctttgacg
      961 tggtcggtga ttccttccag gacggctttg tggaatggca aaagagtgaa tagtcactgg
     1021 ctcattttct ctggatcgga aaactgatat cgaagccggt gacattaccg ttggcatcgg
     1081 tgagtagctt gatatcgccg ccctgacctt tcttttctaa tttttccgta tccacaatta
     1141 aattcttgaa cgccagagcc ttttgcagtt cgttcttgct ttgatccgaa acaatacgat
     1201 tggcgcactc gctcaaactt tcagaaaatg tgccaccggc cggtcccggt ctgtcatcca
     1261 ggattgtagc catttcctgg ggagtgcaaa gcaactggct gacggagctg agatcaggtt
     1321 tcaaccaatt ttggtccggt atatctgcgc ctttatcctg ctcgggacga tctgccatgg
     1381 tcaatacctc gcttgctact gtttgaatgc aagcactatg tcacagccaa tgtgtccgaa
     1441 gtcgggcaat tgccgcggaa accacgtaga caggtaagaa acggctagat aattccggct
     1501 ctcaaaaggt ctttcaaatc aataagtccg accggtcgct gggcgctgtc cacaatcaag
     1561 aggtcggaaa tggaataacg ttccatgagc ttgagagctt ccactgccag gcaatgagca
     1621 gagatggtct tgggagaggt ggtcatcatg tcccgggcct gcttctcgaa gacgccgtcg
     1681 cggtatttgc tcagggcccg gcgtaagtca ccatcggtga tgataccacg caacttgccg
     1741 tcgtcctcgc aaaccacggt gaagcctagt ttctttctgg ttatctcttc cagaaccatt
     1801 tcatggctgg catccagaga aacgatgggc acatccagac cggagcgcat aattgactct
     1861 acggt
//
LOCUS       DAOQNZ010000892         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43715_length_1865_cov_2.988950, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000892 DAOQNZ010000000
VERSION     DAOQNZ010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43715_length_1865_cov_2.988950"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKW73_14545"
     CDS             complement(<1..353)
                     /locus_tag="PKW73_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNA74562.1"
                     /translation="MSQLSEEELCLAEEAARRRTFAIISHPDAGKTTLTEKLLLYGGA
                     IAEAGTVHARRAQRHATSDWMELEKQRGISISSTVLQFEYENSWLNLLDTPGHQDFSE
                     DTYRTLAAADNAVML"
     gene            complement(590..>1865)
                     /locus_tag="PKW73_14550"
     CDS             complement(590..>1865)
                     /locus_tag="PKW73_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNA74563.1"
                     /translation="INNPMQNKKILAVLAVALTASILNPAAARPTMGRGKRLETGNVS
                     TPDRQSREQQGATKFSIDFFKTVMEENAKSGGEKNVVVSPFSLYEVLSMVANGAAGGT
                     REKMAELLGVKPDQLEKLNEHNKANLALLNQNKSVELAVANAIYADNRSPFKKSFTNL
                     CHDYYQAEVENLDFTDEKVLVKINDWCKSKTRGKIAKILDKLSPLEKMVLLNSVYFKG
                     KWDEPFEKNLNTKEPFNTISGASKTVTMMHREDNMPYLKGKGFAAVSLKYAGLNQELL
                     IFLPDKDQNLKAFTQQLTGENFADWQKQLAGKKVALSLPKFTINYGRDLSSSLKAMGI
                     ADAFSATADFSNLIQPPAGACISRVVQKTFIDVNEEGTEAAAVTAGVMGLTSCAMPVE
                     PVQFKVDRPFMLALMDSKTGEVLFLGSIGEPK"
BASE COUNT          343 a          484 c          511 g          527 t
ORIGIN      
        1 atgagcatga cggcattgtc ggcggcggcc agggtacgat aggtgtcttc actgaagtcc
       61 tggtgacctg gtgtgtcgag caggttgagc cagctatttt cgtactcaaa ctgcaaaact
      121 gtagaactga tggagatgcc ccgctgtttc tccagttcca tccagtcgga agtagcgtgt
      181 ctctgggccc ttctggcatg caccgtaccg gcttcggcga tggcgccccc gtagagcagg
      241 agcttttccg tcagagtcgt cttaccggcg tccgggtggg aaataatggc aaaagtacgt
      301 ctgcgggcgg cttcttccgc caggcagagt tcttcttcgc tgagctgtga catcatttag
      361 gttaccccgg catgcagacc ctcgatttta ccgcgacccg ggtgcagcgc ctgcagttgc
      421 taaggccctt gccgccttcc cttaatatag ttgccgctaa tgcactggtc ggcgaaagcc
      481 ggtgatttcg tctgaagtcg gctctctcag ccggtttcga tttacatggc tgccgacgac
      541 cgcaatttct cttgcggtag cttcgctaat tctgtcagga attttaactt tatttgggtt
      601 cgccgatact gcccaggaag aggacttctc cggttttgct gtccatgaga gccagcataa
      661 acggtcggtc cactttgaat tgtacgggtt caacgggcat ggcgcaggag gtcagcccca
      721 tcaccccggc agtgacggcg gccgcttccg tgccttcttc gtttacatcg atgaaagtct
      781 tctgcaccac cctgctgatg caggctccgg ccggcggttg gatcaggttt gagaaatcgg
      841 cagtggcgct gaaggcgtcg gctatgccca tggccttaag gctgctgctc aaatcgcgcc
      901 cgtaattgat ggtgaatttg ggcagcgaaa gagccacttt ctttccggcc aattgctttt
      961 gccagtcggc gaagttctcg ccggtcaatt gctgagtgaa ggctttcagg ttttgatctt
     1021 tatcggggag gaagatcaat agctcttgat ttagtccggc atacttgagg ctgacagcgg
     1081 caaaaccctt gcctttgagg taaggcatgt tgtcttcccg gtgcatcatt gtgactgttt
     1141 tcgacgcacc ggatatggtg ttaaacggct ctttggtgtt caggttcttt tcaaagggct
     1201 cgtcccattt ccctttgaaa taaacactgt ttagcagcac cattttttcc agagggctga
     1261 gtttgtccag gatcttggca atcttgcctc gcgttttgct tttgcaccag tcattgattt
     1321 tcaccagtac cttttcgtca gtgaaatcta gattttctac ttcggcctgg tagtagtcgt
     1381 ggcacagatt cgtaaaggat ttcttgaagg ggctgcgatt atcagcgtaa atggcgttgg
     1441 caacggccaa ctcgacactt ttgttttgat tgagcagagc caggttggcc ttattgtgtt
     1501 cattcagttt ttcaagctgg tcgggtttta cgccgagaag ttcggccatc ttctctctgg
     1561 tacccccggc tgcaccgttt gccaccatgg agagcacctc gtagaggctg aagggtgaga
     1621 ccaccacatt cttctcgccg ccgcttttgg cgttttcttc catcacggtc ttgaagaaat
     1681 caatactgaa tttggtcgcc ccctgctgtt cccggctttg ccggtcggga gtgctcacat
     1741 tcccagtctc cagccgcttg cctcgcccca tagtcggtct ggccgccgcc ggatttaaga
     1801 ttgaggcggt gagggcaact gccaggacgg cgaggatttt cttgttttgc atggggttgt
     1861 ttatt
//
LOCUS       DAOQNZ010000893         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43796_length_1863_cov_4.967920, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000893 DAOQNZ010000000
VERSION     DAOQNZ010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43796_length_1863_cov_4.967920"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKW73_14555"
     CDS             <1..288
                     /locus_tag="PKW73_14555"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNA74564.1"
                     /translation="AGAVVRLFETYRAMSYNLMDVYVSGLSLRTNESTVVLTMIATLF
                     LPPSLIAGIYGMNFNIPEVHVTFGYYFCLASMVVVSGGLLIWLKLKGFIDL"
     gene            310..906
                     /locus_tag="PKW73_14560"
     CDS             310..906
                     /locus_tag="PKW73_14560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA74565.1"
                     /translation="MDELIRRYSELAPDYDRIVTEKAGYTAHARVPQQVLKLLGPLEG
                     ASILDLACGTGLSSDVFFEAGCEVTGIDIAPGMIEVAKLRPFKKLLCQSIEDTLDVPD
                     ASFDAVVAIGVLEFIRAPRQLLEQISRKLRSGGICGLTVPRRLPLHTRLDIFSYSTDE
                     FMSFVDLGCFEVVEVAEFFGYERQQERVEYKAFYLRGS"
     gene            1005..1667
                     /locus_tag="PKW73_14565"
     CDS             1005..1667
                     /locus_tag="PKW73_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74566.1"
                     /translation="MEEKEQSEYLKQLQLELDAPIETVQQFRKAREYCRLISRLGVGN
                     SIAAYVVDELREAATRYLRSGDVEVEEARSAFFESLGPFHFLSDERVLQVCEQLPEVT
                     KNSEDVPIALLYVYEQLLSSRERKQKPWLYNQVRRFACALERHGLLTESLITLSYADE
                     LANLHFDSEEARLNDKLSFYKTDEYRNPLLESLDEVLKKRSLFSSDLKQLKTSIEERI
                     RH"
BASE COUNT          450 a          379 c          526 g          508 t
ORIGIN      
        1 gccggagctg ttgtaaggct ctttgagacc taccgggcta tgtcatacaa cctcatggat
       61 gtctatgttt ccgggctcag cctgcgcact aatgaatcca ccgttgtgct tacaatgatt
      121 gccactctct ttctgccgcc tagcttgata gccgggattt acggcatgaa cttcaatatt
      181 ccggaagtgc atgtcacctt cggatattat ttctgcctgg cctctatggt ggtggtgtcg
      241 ggcgggctct tgatctggct gaagctaaaa ggtttcatcg atctgtagtt gtatcttgca
      301 ggaggaaaga tggatgagct cataaggcga tattccgagc tggcgcctga ctatgaccgc
      361 attgtcaccg agaaggccgg atatacagct catgcaaggg tgccgcaaca agttttgaag
      421 ctattggggc cgctggaggg tgctagtatt ctggacctgg cttgtggtac aggcttgagt
      481 tcggatgtgt tttttgaagc cggttgcgag gtgacgggca ttgatattgc tcctggtatg
      541 atcgaggtag caaaactgag accatttaaa aaactgctct gtcagagtat agaagatact
      601 ctggacgtgc cggatgctag cttcgatgcg gtggtcgcaa tcggagtcct ggagtttatc
      661 cgtgcgcccc gccagttgct tgagcagatc tccaggaaac ttaggagtgg cggcatttgt
      721 ggtcttacag tgccccggcg tctgccattg cataccaggt tggatatatt ttcctattcc
      781 acggatgagt tcatgagctt tgttgatttg ggctgttttg aagtggtaga ggtggctgag
      841 ttctttggct atgagaggca gcaagagaga gtcgagtata aagcctttta cttgcgagga
      901 agttagccac aacatcaaca tggaagcgca gttaattgat aattgagtct gtttcagtgc
      961 acccttcggc cgcctgaaag tggggatata ggagctgaat tgagatggaa gaaaaagagc
     1021 agagcgagta tttaaagcaa ctgcaattag agttggatgc gccgattgag accgtgcagc
     1081 agtttaggaa ggcgcgagaa tactgccgtt tgatcagcag gctgggggtt ggcaattcga
     1141 ttgccgctta cgtagtcgat gaattacgtg aggcggcaac gcgatatttg aggagcggcg
     1201 atgttgaagt agaggaggcc aggtctgctt tctttgaatc cctcggacct ttccacttcc
     1261 ttagtgatga aagggttttg caagtttgtg agcagttgcc tgaagtaact aaaaattcgg
     1321 aggatgttcc gattgctttg ctctatgtat acgaacagct actctccagt cgggagcgaa
     1381 agcagaaacc atggctctat aatcaggttc ggcgtttcgc ctgtgcactg gagaggcatg
     1441 gtcttttgac tgaaagtcta atcacactct cgtatgcaga cgaactggca aacttgcact
     1501 ttgatagtga ggaagcgcgc ttaaatgata agctttcgtt ttataagacc gatgaataca
     1561 ggaatccatt gctggagagc ttggacgaag ttttgaagaa acgctcgcta ttcagtagtg
     1621 atttgaagca actgaagaca agcattgagg aacgtattcg gcactgaaag cccggcaaag
     1681 aaataggcac ccccttgtgc gggaggtgcc tactgaattt ttgcagccgt tagtggagca
     1741 atgctctact tagcggaaac gttttttctg accgccggag gcagaagcaa aagctttgaa
     1801 cttgttgccg cggatctgtc aatcaaactg tgtcattagg tttattggtc actcataact
     1861 gat
//
LOCUS       DAOQNZ010000894         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43871_length_1861_cov_5.744740, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000894 DAOQNZ010000000
VERSION     DAOQNZ010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43871_length_1861_cov_5.744740"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKW73_14570"
     CDS             complement(<1..288)
                     /locus_tag="PKW73_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74567.1"
                     /translation="MLFVTVVLPLQTGKGSGTRALALSEKEAESVADQEGFISHINSE
                     GCHIYLATGESGWQASKQDFHDWVKASVQSIEAYYGSFPVPETRIRLYQTNG"
     gene            complement(427..>1861)
                     /locus_tag="PKW73_14575"
     CDS             complement(427..>1861)
                     /locus_tag="PKW73_14575"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA74568.1"
                     /translation="AGSLNLAEVGEIGGKVETLLRVLDPRESTEMEVIWSEINRALAQ
                     GERAVHKAIDDRSDISEEAHVAVRVLLLGDGYQEILPQLEVATITSCSKTNMALSRLK
                     KHTYEACVFDIVHAGRHELFELARQARLPVEGIVPIALIKPNEASSFLSAADELFLGA
                     SSVNSRPESQEEMELIVSRLLKVRQAQKQRVVVVDDDMVLLNFVETVLTAHGFNVRTV
                     NNPLETGLMLDQFKPDLLLLDAMMPGMTGYEVCRMVREEEQWQNLPIVFLTGKSSPEA
                     RSAAFQAGANDFLSKPVLVDELLSRINGQLAKSAQGKESLAKDANTGLLNEDAFVKAA
                     GELLQATTPQTPMAMALIAADDVEGIRLVHGIESLRQALVALGGEIRLRFKAEDVRGK
                     IGYDAYALLLKDTTKEVAADAIALLQEEFTSRSFFGDRGNFKLDFSTGITDSTEAGRG
                     IKPLLKQAYQRMLSVRGTASGRINYAD"
BASE COUNT          392 a          484 c          500 g          485 t
ORIGIN      
        1 cccatttgtc tgataaaggc gaattcttgt ctcgggtacg ggaaaactgc cgtagtaagc
       61 ctcaatagac tggacagatg cctttaccca gtcgtgaaag tcttgcttgc tcgcctgcca
      121 gccgctctcg cccgtggcga gatatatgtg gcagccttcg ctgttaatat ggctgatgaa
      181 accttcttga tctgccacgc tctctgcttc cttctcagac agagccaggg ctctagtgcc
      241 tgaacccttg ccggtttgaa gcggaagcac cactgttacg aaaagcatga ttgaagtcat
      301 gcctatgaat agagctagat tcataagctt caagtgcacc gtttcgccct tgcctcaaga
      361 tgaactaatt ccatcttaca tgaaggtgct gaattgtgcg cgatttgctc aaaggctgat
      421 gaaaactcag tcggcgtagt tgattcgacc gctggcggta ccccgtaccg agagcatcct
      481 ctgataggct tgcttcaaga gtggcttgat tccacgtccg gcctcagtgc tgtcggtgat
      541 accggtggaa aaatcaagtt taaaattgcc tctatctcca aagaagctac ggctggtgaa
      601 ttcctcttgt agaagggcga tggcgtcggc cgccacttct tttgtagtgt ctttgagaag
      661 gagggcatag gcatcgtagc ctattttgcc tcgcacatct tctgccttga agcgcagtct
      721 gatttcgccc cccagggcta cgagagcctg gcggagcgat tctataccgt gcaccagtct
      781 gattccctca acatcgtcgg cggcgatcag tgccatggcc atgggggtct gaggggttgt
      841 tgcctgaagc agttcacctg ctgctttcac aaaggcgtcc tcgttgagca gtcctgtgtt
      901 tgcatcttta gcgagagatt ctttgccctg ggcggattta gccagctgac cgttgatgcg
      961 ggagagcagt tcgtctacaa gtacgggctt actgagaaag tcgttggctc cggcttgaaa
     1021 ggcggctgag cgcgcttccg gagaggattt gccggtaaga aatactatcg gcagattctg
     1081 ccactgctct tcttccctga ccatgcgaca gacttcgtag ccggtcatac ctggcatcat
     1141 tgcgtccagg agcagaagat ccggcttgaa ttgatccagc atgagcccgg tttcgagcgg
     1201 attgttcacg gttcgtacat tgaagccgtg ggcggtgagt acggtttcaa cgaaattcaa
     1261 gagcaccatg tcgtcgtcta ccactactac gcgctgtttc tgtgcctgtc ttacttttag
     1321 gagacgagag acaatcagct ccatttcctc ctggctttca ggtcggctgt tgaccgagga
     1381 agcgcccaaa aagagttcat ctgccgcact gagaaagctg ctggcttcgt taggtttgat
     1441 cagggcgatt ggaacaatgc cttccacagg cagccgtgcc tgtctggcta gttcgaacaa
     1501 ttcgtggcgg ccggcatgca caatatcgaa gacgcaagct tcgtaggtgt gcttcttcag
     1561 tcgacttaag gccatattgg ttttgctgca ggaggtaatg gtagccactt ccagctgcgg
     1621 caaaatctcc tggtagccgt cgcccagaag taagactctt actgccacat gagcttcttc
     1681 gctgatatcg gagcggtcgt cgatggcctt gtgtacggct ctttcaccct gggcaagagc
     1741 gcggttgatt tcagaccaaa ttacttccat ttcggtggat tcccgggggt caagaacccg
     1801 caaaagggtc tcgactttac cgccgatttc tcctacttcg gccaggttga gtgaacctgc
     1861 c
//
LOCUS       DAOQNZ010000895         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43908_length_1861_cov_2.606312, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000895 DAOQNZ010000000
VERSION     DAOQNZ010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43908_length_1861_cov_2.606312"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKW73_14580"
     CDS             <1..405
                     /locus_tag="PKW73_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74569.1"
                     /translation="RIVAKADGKCFVYGSGALGLEIDPKTGDTGVFRLARDFDGNVYQ
                     GDEALSADTGKVVRTIANETTNRVNGDAAIHPPSGDYLAFEHPGFRDVKSIDPLIKDD
                     GRGLPKEVIRSLVPPPPVRRLIPPPAFLAEPH"
     gene            complement(517..1524)
                     /locus_tag="PKW73_14585"
     CDS             complement(517..1524)
                     /locus_tag="PKW73_14585"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA74570.1"
                     /translation="MSISRPPHKLSLLTLAAGLFLWAALPVEPGYCADADSSPPTQIT
                     RAYGPDRAQTLDIYSAKTNTTGATGTNAAGSAATTGATTSSTASKPRLKPIIFAIHGG
                     AWSGGDKEDKAFGKERAAYFVKLGFLYVSINYRLAPANKHPKQLQDLEHALSYVLKNS
                     AEVGGDKTRVYLLGHSAGGHLAALLTASLYKHDSPELMSIKSLILLDPAALDLSQTYQ
                     KADRKDRAILKAVFGDDPARLNSASPLELLRQRSSTATIGSPELSLLIALSNDWRLKK
                     DKAVQFLSACQRAKLADYGQVVMVEERNHNTLIERLGEPGEKLTSLLNHFLKESSDKQ
                     H"
     gene            complement(1499..>1861)
                     /locus_tag="PKW73_14590"
     CDS             complement(1499..>1861)
                     /locus_tag="PKW73_14590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNA74571.1"
                     /translation="IKHEDGNYSFYAHLLKDSIKVKEGDRVTPGQHIATLGNSGNTTG
                     PHLHFHITSGPGTLGADGIPYVFDQFELSGNVSSMEDCLKSDSQGKPIGIVKTGKEGT
                     HKEELVKEGAIVDFAPAP"
BASE COUNT          369 a          437 c          560 g          495 t
ORIGIN      
        1 aggattgttg caaaagcaga cggtaagtgt tttgtatatg gaagtggcgc cctgggtctg
       61 gagattgatc caaaaacagg tgacactggg gtcttcaggc tggcaagaga tttcgacggg
      121 aatgtttatc agggcgacga agcgcttagc gccgataccg gcaaagtagt taggactatt
      181 gcgaatgaga caacaaacag agtcaacggt gatgcggcaa ttcaccctcc cagcggtgat
      241 tatctggcat tcgagcaccc gggttttagg gatgtaaagt ctatagaccc gctgattaag
      301 gatgacggac ggggtcttcc gaaggaagtg ataaggtccc tggtgccgcc gccgcctgtc
      361 aggcggttga ttcctccgcc ggctttcctt gccgaaccgc attaatggtg aattggttcc
      421 tttgcaaggg ctggttagag gttcggattg ctctgccatg ggaagtttga ctcgcgtgat
      481 tctttacgct ttgagtagcg gaaccggggg cgacacttaa tgctgcttgt cgctggattc
      541 tttgagaaag tgattgagta gcgaggtcaa cttttcaccg ggctctccca gtctttcgat
      601 gagggtattg tggtttcttt cttctaccat caccacctgt ccgtagtctg ccagtttggc
      661 tcgctgacag gcggagagaa actgtaccgc cttgtccttt ttcaggcgcc agtcgttgga
      721 aagagcaatg agaagactga gctctggact gccgattgtg gctgtgctac tgcgctgcct
      781 cagaagttcc aggggcgagg cggagtttaa cctggcgggg tcgtcaccga agaccgcttt
      841 gagaatggct ctgtcctttc gatctgcctt ttgataagtc tggcttaaat cgagagcagc
      901 cgggtcgagc aagatcagtg atttgatgga cattagctcc gggctatcgt gcttgtaaag
      961 gctggctgta agcaaggcag ccaggtgacc gccggccgaa tgaccgagca aatagactct
     1021 tgtcttgtcg ccgcctacct cagcactgtt tttgagcacg tagctaaggg catgttcgag
     1081 atcttgcagt tgtttgggat gcttgtttgc tggtgccagc cggtagttaa tgctgacata
     1141 gagaaagccc agtttgacaa aataggcggc tcgctctttc ccaaaagctt tgtcttcttt
     1201 gtctccgccg ctccaggcgc ccccgtgtat ggcaaatata attggtttca gccgcggttt
     1261 tgatgcggtg cttgaggtag tggcgccggt ggtggcagcg ctgcctgcgg cattggtgcc
     1321 tgtagcgccg gtcgtatttg tcttggcgct gtatatatcc agggtctgtg ctcggtccgg
     1381 tccgtaggct cttgttattt gagttggagg cgagctgtct gcgtcggcac agtagccagg
     1441 ctccacaggt agagccgccc acaagaaaag ccctgccgca agagtaagaa gactgagctt
     1501 atggggcggg cgcgaaatcg acaatggcgc cttccttcac cagctcttct ttgtgggtgc
     1561 cttccttacc ggtttttaca atacctatgg gtttgccctg gctatcgctt ttgaggcagt
     1621 cttccatgct gcttacgttt ccactcagtt cgaattgatc aaatacatag ggtatgccgt
     1681 cggcgcccag ggtgccgggg ccgcttgtga tgtgaaagtg aagatgggga ccggtggtgt
     1741 tgccggagtt gcccagggta gctatgtgct gaccgggagt gacccgatcg ccttccttga
     1801 ctttgataga gtctttcagc aggtgagcat agaaggagta gtttccgtct tcatgcttga
     1861 t
//
LOCUS       DAOQNZ010000896         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_43935_length_1860_cov_3.633241, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000896 DAOQNZ010000000
VERSION     DAOQNZ010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43935_length_1860_cov_3.633241"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..483)
                     /locus_tag="PKW73_14595"
     CDS             complement(193..483)
                     /locus_tag="PKW73_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74572.1"
                     /translation="MKTTSNSKPARALLLLSLGLIALLASATEARAGHCNGSFNPPGP
                     AGGVGHGRFWHYNAPGAQGGPGRGWVYNPPGRGNTIRFPAKYRFRMLTAGLR"
     gene            814..1524
                     /locus_tag="PKW73_14600"
     CDS             814..1524
                     /locus_tag="PKW73_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74573.1"
                     /translation="MPRFIASITRGSGAGVVVLASAALLGAFSVNLLSAPAVSAQAAG
                     ANQTIYRDEQDLLQTDQAMINEYKAEERDAEQKLSDEETKNQPYRLYAEKRIQVLKKA
                     KKAGTPVKTGDTPNELMVLQGWLQRDDAYRSKQMAYIAQLDQAIANLRQGQTSTMANL
                     GNDINDMRQSLQDQRDQQQFDNEMKMMKYNTARTEFGTKMRYLDNSLPNYGFGFGYGG
                     YGGYGGYGGGFGLPSGGY"
     gene            1525..>1860
                     /locus_tag="PKW73_14605"
     CDS             1525..>1860
                     /locus_tag="PKW73_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74574.1"
                     /translation="MPSSGDDEKDKKSPGQGNTSPGLSDYQFFQSGSDSSYNFGLGQN
                     QSQSGVNLNSDLPDWFNELSSASAAKVTESDTLPVVEAVPSTAPEEDVLRSVLPDDGP
                     YWIETHSISD"
BASE COUNT          463 a          449 c          537 g          411 t
ORIGIN      
        1 gcgggttttg tccaccgagt tgcaccgggt ggcgggcggt tttctggcgg aacggcagct
       61 ttacctgaat ttcttcaggg accagagact gcgtttggcc gacctggaac cgggcaacaa
      121 aaccggcccg gggccggtat gttgctagat tctagggcgt cactcagcgg accaggcctt
      181 caacgcagaa agctatctga gaccggctgt gagcatgcga aagcgatatt tagccgggaa
      241 acgaatggta ttgccgcggc cgggcggatt atagacccag ccccgaccag ggcccccttg
      301 agcgccgggt gcattgtagt gccagaagcg cccgtgaccg accccaccgg cgggaccggg
      361 cggattgaag ctgccgttgc agtggccggc cctcgcttca gtggccgacg ccaatagggc
      421 gatcagaccg agcgaaagca atagcaaagc cctagctggt ttggaattcg aggttgtttt
      481 cattttcttg gctccttcga tgcaagttca acgaatctat ttgcttaaac gttggaatca
      541 agtcaagagt tcaacctttt tcggattatt tacggattta agaaaagggc gagggcttta
      601 caaaaataaa tgagcctgac ctgaaaacgc ttaacctgtg ggtgttccga gatagaacct
      661 ggttctcaca ggaagctaac atggtttggt ttcctggccg ttcaggggtg gtacatctag
      721 ggtagataaa acggttcgga tgagtaattc atccagaatt tggaaagttg agaatgcgac
      781 ccacaagaaa tgaaagaaaa gaggtccggg aaaatgccta gatttattgc aagtatcact
      841 agaggcagcg gggcgggggt tgttgtgctc gcttctgccg cacttttagg tgcgttttcc
      901 gtcaatctat tgagcgctcc tgcagtgtcg gctcaggcgg ccggcgctaa tcaaaccatt
      961 tatcgagatg agcaggactt gctccagacg gatcaagcca tgatcaacga atataaggcg
     1021 gaagagaggg atgccgagca gaaactctcg gatgaggaga ccaagaatca accctacagg
     1081 ctgtacgctg agaaacgaat tcaggttctt aagaaggcca agaaagccgg cacgcctgtt
     1141 aagaccggag acacccccaa tgagctgatg gtgcttcagg gatggcttca gcgcgacgat
     1201 gcttatcgca gtaagcaaat ggcctatatt gcccagttag accaggctat tgccaacttg
     1261 aggcaggggc agacctccac catggccaat ctgggcaatg atatcaatga tatgagacag
     1321 tccttgcaag atcagcgcga tcagcagcag tttgacaatg aaatgaagat gatgaagtac
     1381 aacacggctc gcaccgagtt cggcaccaag atgcgctatc tcgataacag cctgcccaac
     1441 tacggcttcg ggttcggcta cggtggatat ggcggatacg gtggttatgg aggcggattc
     1501 ggattgccca gcggaggcta ttaattgcct tcttcaggtg atgacgagaa agataaaaag
     1561 tcaccggggc agggtaacac cagccccggt ctgagtgact atcagttttt ccagagcggt
     1621 tccgacagct cctacaattt cggtcttggt cagaatcagt cgcagtcggg cgttaatttg
     1681 aattccgact tgcccgactg gtttaatgag ctatcttccg ccagtgccgc caaggtgacc
     1741 gagtcagaca ccttgcctgt ggtggaggcg gtaccgtcca ctgcaccgga agaagacgtc
     1801 ttacgctcgg ttttgccaga tgatggtcct tactggattg agacccacag cataagcgat
//
LOCUS       DAOQNZ010000897         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44069_length_1858_cov_3.178037, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000897 DAOQNZ010000000
VERSION     DAOQNZ010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44069_length_1858_cov_3.178037"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..419
                     /locus_tag="PKW73_14610"
     CDS             246..419
                     /locus_tag="PKW73_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74575.1"
                     /translation="MPANKHICQTCGFKIPSAAPASDASKGADSKARVFSKLFGFTNS
                     AKSKEQQEKHALG"
     gene            619..>1858
                     /locus_tag="PKW73_14615"
     CDS             619..>1858
                     /locus_tag="PKW73_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74576.1"
                     /translation="MPASLPSPEQAPPGAAASSEKGAVGNWRKILPFLVITAFFSLAV
                     AVISPAGDFPLNDDWSYGEAVRTFLQTGKFVMPTVCAAGFLHILWGSLFCQLAGFSYE
                     SLRWSSLVASLLGAWAFYLTLRSCRLRIREALFLSLLYAGNPIMINLAFGFMSDSTAL
                     ALLCAYFLLLVLSIRKGSRILVLASMLTLLAAISIRQSAVIFALCAPALFLVNWKPGQ
                     KAASATAVEKPAVLDRSALILSLLFLVLPLLSASAVDNWLMGREAAGQSIANHYGLAR
                     TAHRQFLQSLWQEPGQSWPLFLAACGDVLSYLGIFSLPVLFAWSALPVVLGKKRRHWS
                     GGTGILLLASFLPTALAALITVFQRHRLMPFCENVWRVTSVGAQGIMGIAIAGLRRGH
                     KNFLTALSYLCAWHLLACFLA"
BASE COUNT          319 a          513 c          485 g          541 t
ORIGIN      
        1 ttttgtcgtc aagttgtgcc taatttttga ccggtgccgg ttttcacaaa gagcgggaag
       61 ttcctgctgc ttgccccggg gtttcaagga gacttttaga tggaaagtaa gaagcaggta
      121 aaaagttagg taaatccttc actgtgccgt agaccggcgc ccgcacttga tttcctgtgt
      181 tatactgcga attagaaaga gagggatcta tgcaatattt gctctgccca agatgtcaat
      241 ttaagatgcc ggcaaataaa catatttgcc agacttgtgg cttcaaaatt ccctctgctg
      301 cccctgcttc cgatgcctct aaaggcgccg acagcaaagc gcgagtcttc agcaagttgt
      361 ttggtttcac caactcagcc aagtctaaag agcagcaaga aaaacacgcg ctcggataaa
      421 gttctaactg ccgagcggca gcctcaggcg gtactgtatc tggtaccgct tttttgttcg
      481 gcaattttgc ggcgtctcgt cttcatttgg aaactcttcc agggtatgcc ggtagttgca
      541 gtaacggctt tgccatgcct agaatagcgg tttcagatct gcactttact ctccgcgttc
      601 ctacgccagg aaagatcctt gcccgcttcc ttgccatctc ctgaacaagc cccgccgggc
      661 gctgccgcct ctagcgagaa gggcgcagtt gggaactggc gcaaaatctt gccttttctg
      721 gtgattactg catttttctc gctcgctgtg gcggtgatct ctccagccgg tgatttcccg
      781 ctcaatgatg attggtcgta tggtgaggcg gtgcgaacct tcttgcaaac cggcaagttt
      841 gtcatgccca ctgtctgtgc cgcaggcttc ttgcacattc tctgggggtc gctcttctgt
      901 cagttggcag gcttctctta tgagagcctg cgctggtcat cgctggtagc gtcgctcctg
      961 ggcgcctggg ccttttacct gaccttgcgt tcttgccgct tgcgtatccg agaagcgctt
     1021 ttcctgtcgc ttttgtacgc cggtaatccg attatgataa atctcgcctt cggcttcatg
     1081 agcgacagca cggcactggc cctcctctgt gcctactttc tcttgctggt tctttctatc
     1141 aggaagggtt ccagaatatt ggtgcttgcc tccatgttga ctctgttggc cgccatttcc
     1201 attcggcagt cggcggtgat ttttgcactt tgtgcgccgg ccctctttct ggttaattgg
     1261 aagccagggc agaaggcggc ttctgctact gccgtcgaga aaccggccgt gttggaccgt
     1321 agcgctctaa tcttgtccct gctgtttctg gtgctgccgc tcctgtctgc ctccgcggtt
     1381 gataactggc tcatgggcag ggaagcggcc gggcagtcta tcgccaatca ctatggtctg
     1441 gcccgaaccg cccatcgcca gttcttgcaa tctctctggc aggagccggg gcagtcctgg
     1501 cctctctttc tggctgcctg cggcgacgtg ctttcatatc tgggtatttt ctctttgccg
     1561 gttctctttg cctggtctgc actacctgtg gtgctgggta agaaacgacg ccactggtcc
     1621 ggcgggaccg gcattcttct tctcgcttca tttctcccta cggccttagc cgctctcatt
     1681 actgtttttc aaagacaccg gctgatgccc ttttgtgaaa atgtctggcg tgtcacttcg
     1741 gtgggagccc agggcattat gggtatcgcc attgccggtt tgcggcgcgg gcacaaaaac
     1801 tttcttactg ccctctctta tctttgcgcc tggcatttac tggcttgttt tctggcac
//
LOCUS       DAOQNZ010000898         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44181_length_1856_cov_3.139922, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000898 DAOQNZ010000000
VERSION     DAOQNZ010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44181_length_1856_cov_3.139922"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PKW73_14620"
     CDS             <1..750
                     /locus_tag="PKW73_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74577.1"
                     /translation="NRVLVPRGAKLTYSAQNNLAVDPRALTGFKPNDLWGLSFRRLHN
                     NMHENLSYSKHLTSLRMLDISETSVMVDSMPDIDCLPNLLELEARKTNLTADALSKLK
                     RLRALQTLNIKDIEGSPSAVLSKLKGSKEMTVLKVSDLNLSKGDLNFIVSMPNLKELH
                     IHNTSLNDQDLQKLAGLKKLRLLSVTGTELTPAAAAIFKSAMPRLENLEIGLRIDRWK
                     KSEKEALKRLYKKVDFISTDSVNNWKVELSE"
     gene            792..>1856
                     /locus_tag="PKW73_14625"
     CDS             792..>1856
                     /locus_tag="PKW73_14625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74578.1"
                     /translation="MAPGPDNQINLDERRRKTVTRVWGTAGSKPVGAKVQDLKEGTVI
                     GGAFRIIAPIGRGGMGVVYQAVHLSLKRECALKVLSPELVNQVNWKRFQKEAKAIASL
                     SHPCLVQVYDLGIHNSRLPYYAMDLLKGKDLESVIHSKGSLSLPEAISIMLKALDGFA
                     YAHRNGVIHRDIKPANILLTREANGETGVKILDFGIAKLAGFDDDEPDQSMTATGEIF
                     GSPFYMSPEQCMGEPVDARSDIYSLGCTLFEMLTGQVPFEAATSLEIVMMHLEEYPPT
                     LSQKSGRNFPKSIEAVMGKCLAKNPDFRYQNAKELAIDLERISQGKEILPDTSQNAAR
                     SQKAIDMAKKAEQQEAEAGKS"
BASE COUNT          518 a          508 c          450 g          380 t
ORIGIN      
        1 aacagggtgc tggttccgag aggagccaag ctcacttaca gcgcccagaa caaccttgcc
       61 gtcgacccca gagccctgac aggtttcaaa ccgaacgacc tctgggggct atctttccga
      121 cgcctgcaca ataacatgca tgaaaacctg agttactcca agcaccttac cagcctgcga
      181 atgttggaca tcagcgagac ctcggtgatg gttgacagta tgccggatat tgattgcttg
      241 cccaatctgc tcgaactgga ggccaggaaa accaatctta ccgctgacgc cctgtccaaa
      301 ctaaagcgcc tgcgtgctct ccagacgcta aacataaaag acatcgaggg ttcgccctcg
      361 gcagtccttt ccaaactgaa aggcagtaag gagatgactg tcctcaaggt gagcgatctc
      421 aacttaagca agggcgatct gaacttcatt gtcagtatgc ccaacctcaa agaactgcac
      481 attcacaaca ccagcctcaa cgaccaggac ctgcagaaac tggccggact caaaaagcta
      541 agacttctca gtgtcacggg aacagaactg acgcctgccg ccgctgccat tttcaaaagt
      601 gccatgccca ggctggaaaa cctggaaata ggtctcagaa tcgaccgctg gaaaaagtcc
      661 gagaaagagg ctctcaagag gctgtataaa aaggtagact ttatatcaac agactcagta
      721 aacaattgga aagtggagct ttccgagtaa tctcagagca ttgagtaaag acttcttctc
      781 ttggtcgcac tatggctcca ggtccggaca atcaaatcaa cctggatgag cgcaggcgca
      841 agacagtaac gcgtgtctgg ggaacggcag gcagtaagcc ggtcggtgcc aaggtgcaag
      901 acttaaaaga aggcaccgta attggtggcg ccttccgtat cattgccccg attggacgcg
      961 gcggtatggg tgtcgtctat caagccgttc acctctctct caagcgcgaa tgcgccctca
     1021 aagtcctctc tcccgaactg gtaaatcagg tcaactggaa gcgcttccag aaagaagcca
     1081 aggctatcgc ctcactttct catccttgcc tggtgcaggt ttacgacctg ggtattcata
     1141 actcccgatt accttattac gccatggatt tgctcaaggg caaagatctt gaatcggtga
     1201 tacatagcaa gggctcactc agtctgccgg aagccataag tattatgctc aaggccctgg
     1261 atggcttcgc ctacgctcac agaaacggtg ttattcacag agatatcaaa ccggcaaata
     1321 tactactgac cagagaagcc aacggtgaaa ccggcgtaaa aattctggac ttcggcatag
     1381 ctaaactggc aggttttgat gacgatgagc cggatcaaag catgaccgcc accggtgaaa
     1441 tctttggcag ccccttttac atgagcccgg aacagtgcat gggagagccg gttgacgccc
     1501 gctcggacat ctattctctc ggctgtactc tctttgaaat gctgaccggc caggtgcctt
     1561 ttgaagccgc cacctctctg gaaatcgtca tgatgcatct ggaagagtac cccccgaccc
     1621 tctcccagaa aagtggtcgc aacttcccca aatcaatcga agcggtaatg ggcaagtgcc
     1681 tggccaaaaa tcccgatttc cgctaccaga acgctaaaga actggcaatc gacctggaga
     1741 gaatcagtca gggcaaagaa atcttgcccg atacttccca aaacgccgct cgctctcaaa
     1801 aagccataga catggccaag aaagcggaac aacaggaagc ggaagctgga aaatcc
//
LOCUS       DAOQNZ010000899         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44216_length_1855_cov_4.063889, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000899 DAOQNZ010000000
VERSION     DAOQNZ010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44216_length_1855_cov_4.063889"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKW73_14630"
     CDS             complement(<1..206)
                     /locus_tag="PKW73_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74579.1"
                     /translation="MALFRSRRAISLCLLLALAVPAARAQTAGNWLKLEQHSELFGNY
                     DIYICQDSFVLHTLESDVNVVFKPP"
     gene            453..1358
                     /locus_tag="PKW73_14635"
     CDS             453..1358
                     /locus_tag="PKW73_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA74580.1"
                     /translation="MPKDGNSTIGRKILRLTLFFFAVVALVIASTYLFFAPVFHSDYI
                     DSMLLHPLKNSADAPVQDQVLGIRAEAAQVPVSRGNKEVLISAAHYKLPHPKGLVIFS
                     HGTGGNIDIRFSKQNPRLSMLLKQGLEVLLYDYEGYGRSPGKASLRNLKDDCLAVYNY
                     ALKLGFKPEKIFLYGESLGGGVSCMLAQEKPVRAIILDCTFTSPERLAKELMPLAEIY
                     PSFLFPEPRLDNLHYLQGNHPPCLIVAGKKDPTIPSSQSILLRRMAKPSADLLILPES
                     YHCYLAESDLKTFEATLSKFLNKLI"
     gene            complement(1371..>1855)
                     /locus_tag="PKW73_14640"
     CDS             complement(1371..>1855)
                     /locus_tag="PKW73_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74581.1"
                     /translation="VFGAGLTGFLVLCFGRQFTISLAFTMTLVLCQLIFNTSYSFDGG
                     FLTLLFLCGFFSIMSATTICIYVPEIFPLPIQGTAFGLAFNFGRFFAAVIALVSAQLI
                     ASFNGSYASAASALSYIYVIGIVASCFMTKTSGEVLLDVTESGKAEVPEQEERLLTGV
                     "
BASE COUNT          504 a          474 c          449 g          428 t
ORIGIN      
        1 ggcggcttga acaccacatt gacgtcggat tcaagagtgt gcagaacaaa actatcctgg
       61 caaatataaa tatcgtagtt tccgaacaat tcgctgtgct gctccaactt cagccagttg
      121 ccggccgtct gtgcgcgggc tgcaggaaca gcaagtgcca gtaacagaca tagagaaatt
      181 gcccttctag acctgaataa agccatggaa ttacctcatg ctatttcaat tctagtggaa
      241 ggtgaaaagt taagccgtct tatagcgccc atgtaaggag aaacttttgc caattaatct
      301 gtccttccct gacagttttg ccggaccggt tcctatggac ccgtctggaa gccggcgctg
      361 agtgatccgt cacttaataa actgtttcag gacagcctga ccgtgagttg ccggtgctac
      421 aacggaaaga gtgccttcaa tgggagctaa ccatgccaaa agacggaaac tcaaccatag
      481 ggcggaaaat cctgcgtctg accctatttt tctttgcggt ggtagctctg gtgattgctt
      541 ccacctactt gttttttgca ccggtctttc atagcgacta catcgactcc atgcttctgc
      601 atccactcaa aaattcagcc gacgctccgg tgcaggacca agttctgggt atcagggcag
      661 aggcggcgca ggtaccggtc agtcgcggca acaaggaagt gcttatctcc gccgcccact
      721 acaaattgcc ccatcccaaa ggtttggtca tcttttccca tggcacaggc ggcaatatcg
      781 acattcgctt ttccaagcag aacccccgac tttccatgct cttgaaacag gggctggaag
      841 tgctgctcta cgactatgaa ggctacgggc gctcaccggg caaggccagc ctgcgcaatt
      901 tgaaagatga ctgcctggca gtctacaact acgccctcaa gcttggtttt aagcctgaga
      961 aaatcttcct ctatggtgaa tccctgggag gtggcgtctc ctgtatgttg gcccaagaga
     1021 agccggtacg tgccattatt ctggattgta catttacatc gccggagcgt ctggctaaag
     1081 aacttatgcc tctggcggaa atttatccaa gttttctttt ccccgaacct cgtctcgata
     1141 acctgcacta cctgcaaggg aaccatcctc cttgtttgat cgttgcgggc aaaaaagatc
     1201 ccacaatacc gtcctcgcag tctatcctgt tgaggcgaat ggccaagccc agtgccgatc
     1261 tgcttatact gccggaaagc taccattgct atctggcaga gagtgatctg aaaacctttg
     1321 aagcgactct atccaagttc ctaaacaagt tgatttgagc agcaagcaat tcagacacct
     1381 gtgagcaatc tctcttcctg ctcaggaacc tctgctttgc cagactcagt aacatcaagg
     1441 agcacttcac cgctggtttt tgtcataaaa caggaagcaa caatgcctat tacatagatg
     1501 tatgaaaggg cagaagcagc tgaggcatat gagccgttga aactggcaat gagctgagcg
     1561 cttacgagcg cgatgacggc agcaaaaaag cgaccgaaat tgaatgccag accaaaagca
     1621 gtgccctgaa tgggtagggg aaaaatctcg ggcacgtaaa tgcaaatcgt tgtggcactc
     1681 ataatggaaa agaaaccgca gagaaagagc agagtcaaga agcctccatc gaaactgtag
     1741 ctcgtgttga aaatgagctg acagagaaca agtgtcattg taaaagccag gctaatagtg
     1801 aattgacggc cgaaacaaag cacgaggaag ccagtcagac ccgcgccaaa aacgc
//
LOCUS       DAOQNZ010000900         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44364_length_1852_cov_3.750139, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000900 DAOQNZ010000000
VERSION     DAOQNZ010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44364_length_1852_cov_3.750139"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKW73_14645"
     CDS             complement(<1..387)
                     /locus_tag="PKW73_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74582.1"
                     /translation="MTNSLEQKLDLLFSKYEKLQASLCLVNNIRDPVITDSPLLLATA
                     QARSTINGFLSRISEDDDEIKKLSLLLDEAWDQLMPCLLAFVERQQLAAFDQASIDQD
                     CEHFQGILPLDEDGLLSCELDELLDAI"
     gene            complement(459..803)
                     /locus_tag="PKW73_14650"
     CDS             complement(459..803)
                     /locus_tag="PKW73_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74583.1"
                     /translation="MNEQIAMLSPVILMVVGPVGLVVTARTLYLALRRFCKLAPAPAI
                     AAAGIVLLLAAAMLYLVFGLDKAVFLLCIFGASEVIIMGTPLAKYHVLLKLDSAVAAR
                     VGQGNSSWGVKP"
     gene            complement(1374..>1852)
                     /locus_tag="PKW73_14655"
     CDS             complement(1374..>1852)
                     /locus_tag="PKW73_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74584.1"
                     /translation="IAEIGTRRKTADLTLHERALNTDTREAITEESVGKLIEQLTLSS
                     DRLQGAMHRIGFLESQIESMEGQLSFLPDFRAKAARCIVLEKENQELKAVIEHRNLQL
                     LKRERLLDERNQQLSILEKILAAYKKHLSMVEADLSRLENSSWVRFCAWFTGSPLK"
BASE COUNT          418 a          443 c          524 g          467 t
ORIGIN      
        1 aatggcatca agcagttcgt caagctcgca ggacagaagc ccgtcttcat caaggggcag
       61 aatgccctgg aagtgttcgc agtcctggtc aatggaagcc tggtcgaaag ccgcaagctg
      121 ttgccgttcg acaaaggcga gcaagcaagg catgagctgg tcccaggctt cgtccagtaa
      181 gagcgaaagt ttctttatct cgtcgtcgtc ttcggaaatg cggctcagga agccgttaat
      241 tgtgctgcgc gcttgtgctg tcgccagaag caagggactg tcggttatga ccgggtctct
      301 gatattgttc accaggcaaa gtgatgcttg cagtttctcg tatttgctaa acagaaggtc
      361 gagcttttgc tctaagctat tggtcatttg tactcctctt tttagaaaac tttgacggct
      421 ctgaattgag cagaccgccg ggttcgccct tgcaagactc agggtttgac tccccaggag
      481 ctatttcctt ggcctacgcg agcagccaca gccgagtcta gtttcaagag cacatggtat
      541 ttcgccagcg gtgtgcccat gatgatcact tcggaggcac cgaagatgca caggagaaag
      601 acagccttgt ccagaccgaa gactaggtaa agcatggctg ccgccagaag cagtacgatt
      661 cctgctgcgg ctatggcggg agcgggggca agtttgcaaa aacggcgcag ggccaggtaa
      721 agggttctag ctgtgacaac cagaccaaca ggtccgacca ccatgagaat taccggagag
      781 agcattgcga tttgttcgtt cataagaagt cctcgattgt taatgaattg tcagagccgt
      841 gagcatcagc aggagcgctt ccggcaatat gaaaatgccc cgatgtaagt ttcgagacgg
      901 ttgtaaccgc agttcaatcc cgttgtgaat agcaaaaccg cacagcaggc tgactctgaa
      961 gcaaactagt tgcatagaga aaaaacatgt tgccgttctg gctggtgttt gtgtgtggtt
     1021 taaaaccttg agtctggaaa gagatgtgaa aagcgggata acttgacctt actgagactt
     1081 ttctgaggct gcaagaaagg ccaaagcttc ggagacgccc gggctttcag cctgaaaagc
     1141 taatgcagcg gtgttttcgg gcaccgctgt aagcaacttt ttgaaagtct gtcatggatg
     1201 ccgtggtggt actacgagtc aataacagtt agcgtgtgct aaggctgatc cggttcttca
     1261 agacgcttat tcagtgagcg ttttgagcac ttccttgcca ggcggcgctg acggcgtctc
     1321 tctctggagc cgtccaagtg gactgatttg cccggtgccg cgagcggctc aatttacttg
     1381 agggggctgc cggtgaacca ggcgcagaat cttacccagc tgctgttttc caggcggctg
     1441 agatctgctt ccaccatgct caggtgcttt ttgtaagcgg caagaatttt ttccaggatg
     1501 ctcaactgct gattgcgctc atcgagcaag cgctctcttt tgagaagttg tagattgcgg
     1561 tgttcgatga ctgctttcag ttcctggttt tctttctcaa ggacgatgca tctggctgcc
     1621 ttggctctga aatcgggcag gaaggacagc tgcccttcca tggactcaat ctggctctca
     1681 agaaagccga tgcggtgcat ggcgccctgc aagcggtcgg agctcaaggt gagctgctcg
     1741 atcaattttc cgacgctctc ttccgtgatg gcttcacgtg tatcagtatt gagagctctt
     1801 tcgtgcagag taagatcggc ggtttttctt ctggtgccga tctcggcgat gg
//
LOCUS       DAOQNZ010000901         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44427_length_1851_cov_2.956013, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000901 DAOQNZ010000000
VERSION     DAOQNZ010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44427_length_1851_cov_2.956013"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..>1851
                     /locus_tag="PKW73_14660"
     CDS             31..>1851
                     /locus_tag="PKW73_14660"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74585.1"
                     /translation="MTKTAGVERIEIVLKTYLAQAEKMMGDVAEAAASFEQVIERASR
                     EEFLDLAILARVFLAELLKEKGDYTRARHHFEHAYNCASFLKDAVSMELSAANLGSVS
                     FSLGHLEQACGWFKTAVDLYEQRSQDNGGSLSIWLGSLGLTMSELGQFNKAVEFYEKA
                     YALASSQDDLLTMSICRGSIGNVCFEQKDYDGALSAYEEARQLAGKVNDQRREGIWLG
                     NVGIAMSRNGDQEGAILKLSEALTLARSLDDKQSMAAHLDSLGDCARAKGDLEQSRQY
                     YEEAIALARLVSDRLGERIYLSNMARLKRDCGELSPAFEYFAEAVDLFDEQRGFIKAD
                     DLKTSFADRGQELYRDVIATCLDMGKRVEALEFVGRAKSRALLDLLSNSPIDVADLGV
                     TGSADLDGSLKTLIAREADLRAQIAQLERLFWQGDGPSPGVGHYRGVQISQEDTRKLY
                     GEWRDVLNQLKRKHPNYASLVSSTTLKYSEIQALWRDGQLKEDTCVIEFFLPGDYLLV
                     AAAVSDKLRAEQDGVTLEEPLTHLLMVESELNALREDVQTFLEMSSTEGWEVPLSLCK
                     RLYNRLLAPVIEKLPAGITRLILVPHGVLYHLPFSALHDGK"
BASE COUNT          425 a          495 c          519 g          412 t
ORIGIN      
        1 gccaagccgg aactcgaaag agccattgac ttgactaaaa ccgccggtgt ggagcgcatc
       61 gaaattgtgc tcaagaccta tttggctcag gctgaaaaaa tgatgggcga tgtggcggaa
      121 gcggccgcct ctttcgagca ggtgattgaa cgggcttccc gtgaggaatt cctcgatctg
      181 gctatactgg cgagagtctt cctggccgag ctgctgaagg aaaagggtga ttacacaagg
      241 gcccggcacc acttcgaaca cgcctataac tgtgcttcct ttctcaaaga tgccgtctca
      301 atggaactca gtgccgccaa tctgggttcg gtttccttca gcctgggcca cctggagcag
      361 gcttgcggct ggttcaaaac cgccgtagac ctttatgagc aacgctcgca agataatggt
      421 ggcagcctct ctatctggct tggcagcctc ggtctgacca tgagtgaact gggtcagttc
      481 aacaaagccg tcgaattcta cgagaaagct tatgccctcg cttccagcca ggacgacctt
      541 ctcaccatga gcatctgccg tggttcgatt ggaaatgttt gcttcgagca gaaagactat
      601 gacggtgccc tcagtgcata cgaggaagcc aggcaactgg ccggcaaagt caatgaccag
      661 cgtcgggaag gcatctggct tggtaatgtg ggcatcgcca tgagccgaaa cggtgatcaa
      721 gaaggcgcta ttctcaagct ttcagaagcc ctgaccctgg cccgttccct tgatgataag
      781 cagtccatgg ccgcccatct cgacagcctg ggtgactgcg ccagagctaa aggcgatctg
      841 gaacaatcca ggcagtatta cgaagaggcc atcgctctgg ccagactggt cagtgacagg
      901 ctgggcgagc gcatttacct gagcaatatg gcccgcttga agcgcgattg cggcgagttg
      961 agcccggcct ttgaatactt tgcggaagcc gtcgatctct tcgatgagca gcggggtttc
     1021 atcaaggccg atgacttgaa gacaagcttt gccgatcgcg ggcaggagct atatcgcgat
     1081 gtaattgcaa cctgcctcga tatgggcaaa agggttgaag ccctcgaatt tgtcggcagg
     1141 gctaagtcaa gagcgctgct ggacctgctc agcaattccc ctattgatgt agctgatctt
     1201 ggtgtcaccg gcagtgccga tctggatggc tccctgaaaa ctttgatcgc cagggaagcc
     1261 gacctgcgcg ctcaaatcgc tcaactggag agactcttct ggcagggaga cgggccttcg
     1321 cccggtgtcg ggcattaccg cggcgtccag atcagtcagg aagatacgcg caaactctac
     1381 ggcgagtggc gagacgtcct caaccagttg aagcgcaagc atcctaacta tgcctctctg
     1441 gtttcttcca ccacactcaa atacagtgag atacaagctc tctggcggga tgggcagctc
     1501 aaggaagata cctgcgttat agaatttttc ctgcccggtg attatctact cgtggctgct
     1561 gccgtcagtg acaaactgag agccgagcag gatggcgtca ctttagaaga accgctcacc
     1621 catttactta tggttgagag cgaactgaac gccctcagag aagacgtgca gacttttctg
     1681 gagatgagtt cgacggaagg ttgggaagtg ccgctcagcc tctgcaaacg cctttacaat
     1741 cggcttcttg ctcctgtgat tgaaaagttg ccggccggca tcaccaggct tatattagtg
     1801 ccgcacggtg tgctctatca tcttcctttc agtgccctgc acgacggcaa a
//
LOCUS       DAOQNZ010000902         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44586_length_1848_cov_2.500837, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000902 DAOQNZ010000000
VERSION     DAOQNZ010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44586_length_1848_cov_2.500837"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..948
                     /locus_tag="PKW73_14665"
     CDS             40..948
                     /locus_tag="PKW73_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HNA74586.1"
                     /translation="MGTGGVGAVFGARLAHAGEDVTFVARGKHKEAIVANGLQIKSAE
                     RGDLLIKPCRVVSAFEQEPPFDFIFVTVKLWDTETAIETIRPVVGENTTVISFQNGIS
                     RDELLQAAFGAHRVMGGISYVGATISAPGLIEQKGTVQKLVFGEYDGTISPRALSLKA
                     ALEKADIEAVVVTDILSSLWQKFVVLVAMSSLTASVRQPIGPVRANAKTRELLQRVME
                     EVVAVATASGVNLPADLVSDRMAYLDGLAPDVTASMEYDLRVGNRLELPWLAGTVVAL
                     GKKLAVATPVSSTLCAILDPYVNGRS"
     gene            951..1511
                     /locus_tag="PKW73_14670"
     CDS             951..1511
                     /locus_tag="PKW73_14670"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNA74587.1"
                     /translation="MVRTYSTMLELGTAAPHFSLPDVVSGKTITLEDFSTCRALLVMF
                     ICRHCPYVKHIQSELSRLGADYNDSGLGIVAISSNDAEAYPADSPESLKEFALEEKLN
                     FPICYDQDQSVARRYEAACTPDFFLFDANKRLVYRGQLDDSRPSSEIPVTGADLRNAI
                     DAVLCGSPCASEQKPSLGCNIKWKQS"
     gene            1622..>1848
                     /locus_tag="PKW73_14675"
     CDS             1622..>1848
                     /locus_tag="PKW73_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74588.1"
                     /translation="MLDGVCEPAVAIATELELCDASIFIGKYEFAGPFDKPADIAEER
                     GVFAVLCYHDREYELLDIGFADNLRKALMEHP"
BASE COUNT          396 a          477 c          495 g          480 t
ORIGIN      
        1 ccaggcatca ataagtaggt ctctatgcga attgcaatta tgggaacggg tggagttggt
       61 gccgtatttg gtgctagatt ggctcatgcc ggggaggatg tcacttttgt ggcccgcggc
      121 aagcataaag aggcgattgt cgctaacggt cttcaaatca agagtgccga gcgcggcgac
      181 cttttgatta agccctgtcg agtggtctct gcttttgagc aggagccacc cttcgacttc
      241 atctttgtta ccgtcaagct ctgggatacc gagaccgcta ttgagaccat cagacctgtt
      301 gtgggtgaaa acactaccgt tatttccttc cagaacggca tcagccgcga tgaacttttg
      361 caggctgctt ttggggcgca tagggtcatg ggtggtatca gttatgtcgg tgccaccatc
      421 agcgctcccg gcctgattga gcaaaaagga acggtgcaga aactggtttt tggggaatac
      481 gacggcacca tttccccgcg ggccctttcc ttgaaggcgg ccctggaaaa ggccgacatc
      541 gaagctgtgg tggtaaccga tattctttct tcgctctggc aaaaatttgt ggtgcttgtg
      601 gccatgagtt ctctcaccgc cagtgtcagg cagccaattg ggccggtgcg agccaatgcc
      661 aagacaaggg agttgttgca gcgggtcatg gaggaggtgg tggcagttgc cactgccagc
      721 ggtgttaacc ttcctgccga cctggttagt gatcgcatgg cttaccttga cggtctggct
      781 cccgatgtta ctgcttccat ggaatacgat ttgcgtgtgg gcaataggct ggagttgccc
      841 tggctggccg gcacggttgt agcgcttggt aaaaaacttg cggtggccac tccggtttcg
      901 agcacactct gtgccatact tgacccttac gttaatggga gaagttaaaa atggttcgta
      961 cctattcgac tatgctggaa ctcggtaccg ctgctcccca cttcagcttg cctgatgtgg
     1021 tctccggcaa gaccatcact ctggaagact tctctacctg ccgcgctttg ctggtgatgt
     1081 tcatttgccg tcattgccct tacgtcaagc acattcaaag cgagctttcc cggctaggtg
     1141 ccgattataa cgacagtggg cttggcattg tggctatttc aagcaatgat gctgaggctt
     1201 accctgctga tagcccggag tcgctgaaag agttcgctct ggaagagaag ctcaattttc
     1261 ccatttgcta tgaccaggat cagtcggtag ccaggcgcta tgaagccgct tgcacccccg
     1321 acttctttct ctttgatgcc aacaaacgcc ttgtttatcg cggtcaacta gatgacagcc
     1381 gcccttccag cgagattccc gtcaccggtg ccgaccttcg caatgccatc gacgccgtgc
     1441 tttgcggttc gccctgtgcg tccgagcaga agcccagcct tggctgcaac attaagtgga
     1501 agcaatcgta aattacacaa ttgcaaaaca tcttgttaac cgattcgcct ctaacatgtt
     1561 gtcactgtcg gaaaaacgat cattcgccga ctcttacaat ctaaattaag aggggaacat
     1621 catgttggat ggtgtttgtg agcccgccgt cgccattgcg accgagcttg aactttgtga
     1681 cgcttccatt tttatcggca aatacgaatt tgccggacct tttgataaac ccgctgatat
     1741 tgccgaggaa cgcggcgtgt ttgccgtact ctgctaccac gacagagaat acgaacttct
     1801 ggatatcggc tttgccgata atctgagaaa agccctcatg gagcatcc
//
LOCUS       DAOQNZ010000903         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44607_length_1847_cov_4.470982, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000903 DAOQNZ010000000
VERSION     DAOQNZ010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44607_length_1847_cov_4.470982"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKW73_14680"
     CDS             complement(<1..622)
                     /locus_tag="PKW73_14680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74589.1"
                     /translation="MNGENPQSKNNPNQRSLTGALIDDRFYVQSLLGCGATSSVYRAR
                     DKQEERDVAIKILHEHLSHNELLLARFKQEAHAASILKHPNIVSIYNYAVSSGVPYLT
                     MELVEGQTLQEVLTGVGWLPLTRAMPIFQQICAALTSAHELSIIHRDLKPGNVILSKT
                     DSEQELVKILDFGSAQLAPMLSDTVLKLTQTGEMLGSILYMSPEQCL"
     gene            complement(637..984)
                     /locus_tag="PKW73_14685"
     CDS             complement(637..984)
                     /locus_tag="PKW73_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74590.1"
                     /translation="MLKHSALLLSTAALLATSSNLLVQDAAMAKGKAARSRQGHAYLV
                     PPPPAYAPSILPELRRQASHPTGTNSRVIEEADASQAESTEAAKKYIYSTNGQEPKAV
                     RQNKYVTIWNSKS"
     gene            complement(1107..1523)
                     /locus_tag="PKW73_14690"
     CDS             complement(1107..1523)
                     /locus_tag="PKW73_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74591.1"
                     /translation="MISFQNFLQQIKQIIKTSNHGQTVQAKEAAEVNPWQHNLERLAE
                     KHGSELKETNPQTVEEQFQVLIEQAKMDKTKDALRKQQEILQKGGQRSARINDKTVTD
                     AKAWWGQAVATWDPEDSKNVTMEDRRIPRQHKWFNQ"
BASE COUNT          358 a          429 c          531 g          529 t
ORIGIN      
        1 ccaggcactg ttcggggctc atgtagagta tgctgcccag catttcgccg gtctgggtca
       61 gttttagcac ggtgtcactg agcatgggtg ccagttgggc cgagccgaag tcgagaattt
      121 tcaccagttc ctgttcactg tcggttttgg aaagtattac gttgcctggt tttagatctc
      181 ggtggatgat ggagagttcg tgggcgctgg taagggccgc acagatctgt tggaagatcg
      241 gcatggccct ggttagcggc aaccagccca ccccagtgag aacttcctgc agggtttggc
      301 cttctacgag ttccatggtc aggtaaggca cacctgagga tacggcgtaa ttgtagatcg
      361 atactatatt gggatgtttg agaattgagg cggcatgggc ttcttgcttg aagcgcgcca
      421 gaagtagctc gttatgggag agatgctcgt gcagtatttt gatggcgaca tcacgctctt
      481 cttgcttgtc tctggctcgg tacactgaac tggtggcacc gcagcctaag agcgactgca
      541 cgtaaaaacg gtcatcgatg agcgcgcccg tcaatgaccg ttgatttgga ttgtttttcg
      601 actgtggatt ttcgccgttc atcaacagcg gctaggctag cttttgctgt tccagatagt
      661 gacgtatttg ttctggcgaa cagctttggg ttcctggcca ttggtgctgt aaatgtattt
      721 cttggcggct tcggtagatt ccgcctggct ggcatcggct tcttcgatta cccggctgtt
      781 ggtgccggtg ggatgggagg cctggcggcg cagttccggc aaaatggacg gcgcataagc
      841 cggtggaggt ggtaccaggt aggcgtgccc ctgccgagag cgtgccgctt ttcctttagc
      901 catggctgca tcttgtacta acagattgct ggatgtagcc agcaaggctg ccgtggaaag
      961 tagcaaagcc gaatgtttta gcatcagacc cccatttaac gccatcaaat caacgattta
     1021 agttggcgtt ttatctatgc cgttgcaaat acctgcaccg atcataccac caggatcttg
     1081 acctcaaact gaggataact tttgctttac tgattgaacc acttgtgttg tctgggaatg
     1141 cggcggtctt ccatcgtcac gtttttgctg tcttccgggt cccaggtagc cacggcttgt
     1201 ccccaccagg ctttggcgtc ggttacagtt ttgtcgttga tacgggcaga gcgctggcca
     1261 cctttttgca gtatttcttg ctgctttcta agggcatcct tggtcttatc catcttggct
     1321 tgttcgatta gaacctggaa ctgttcttcc accgtttgcg ggttggtttc tttcagttca
     1381 ctgccgtgtt tttcagccag gcgctccaga ttgtgctgcc aggggttgac ctcggcggcc
     1441 tccttggctt gcacggtctg gccgtgattg ctggtcttga taatctgttt gatttgttga
     1501 agaaaattct ggaaggaaat catagtaaga aaactccggg agcaatattg agtagtttca
     1561 agcgatagat aggtttttag agtagagccg gacctgttaa gttaattaga gcgccggatt
     1621 ttgcgttgac ttgtggtgta ttcccatcga gctagggcgg taacgcctgc cggattcaga
     1681 ttaagcggtg ttaagagctt cttcgtatct gatgctcgcc cgtatcagca ataacagaca
     1741 gccctccagg tttaggaggg ctgtcaagaa gggtgatggt ttgtaacgga ttcttagctg
     1801 gccatctcta cggtttcgcc ggccagcacc agggtaggca ctgcctg
//
LOCUS       DAOQNZ010000904         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44616_length_1847_cov_3.356585, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000904 DAOQNZ010000000
VERSION     DAOQNZ010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44616_length_1847_cov_3.356585"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PKW73_14695"
     CDS             <1..518
                     /locus_tag="PKW73_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74592.1"
                     /translation="VDTLQFKILIPQSEANHAEKPPSPIGRKLIARLDKSFSTILEAG
                     AKPAAQAGTLRQLEASIIDCTELKEGEYKLWKHGRYYIFFICGKCGGSVSLSEGCQHC
                     HTAYGALENCEELASEHLCDHRLPLPAPVARFVKANKLWQFQQDPEEARASTLAAQEP
                     VKGKPKLNKTK"
     gene            complement(732..1085)
                     /locus_tag="PKW73_14700"
     CDS             complement(732..1085)
                     /locus_tag="PKW73_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74593.1"
                     /translation="MRDSKSDARHLRIFGAYEGFLAGARQVRDLAPGPDCVPACALEP
                     MRRFIDAAAQFQQVLASEYNSSEAGQYWSQYLQGHLENGRRLFAGGAVKPADAYDNFG
                     YLHNGIVDAFDELTA"
     gene            complement(1143..1559)
                     /locus_tag="PKW73_14705"
     CDS             complement(1143..1559)
                     /locus_tag="PKW73_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74594.1"
                     /translation="MDDMTIANLHTATKAIYDRGERALKQARELAHGEDAKATAILLC
                     EEARSVHREAIGLKVDVQHHENAYPTKAIFNFRLWRVMDAVAFALSAEAACTRVLGDA
                     TMADLLEKQAADALAAAVEYHDRARAAERELGKGKR"
BASE COUNT          437 a          546 c          484 g          380 t
ORIGIN      
        1 gggttgacac cctgcaattt aagattctca ttcctcagtc cgaggctaac catgcggaaa
       61 agccgccctc acccatcggt cgaaaactga ttgctcgcct ggacaaaagc ttcagcacca
      121 ttcttgaagc aggtgccaaa cccgctgctc aagcaggcac cctcaggcaa ctggaagctt
      181 caattatcga ctgcactgag ctgaaggaag gtgagtataa gctctggaaa cacggccgat
      241 actacatctt cttcatctgc ggcaaatgcg gcggcagtgt cagcctgagc gaaggctgtc
      301 agcactgcca caccgcctat ggtgcgctag aaaattgcga agagctggct tccgaacatc
      361 tctgcgatca ccgcctgccc ctgccggcgc cggtggccag atttgtgaaa gccaacaaac
      421 tctggcaatt ccagcaagac ccggaagaag ctcgtgcgag cactctagcc gcacaagagc
      481 cggttaaagg gaagccgaaa ctgaataaaa ccaagtaaca ctaagaactt gaaaggaggc
      541 gcaaattccc tagcgcttcc tttctccttt tcctgttgca gaaaacaccg tctcgacaaa
      601 ctgctttttg aatttcgtca tcttactatt tcttatcgta ttttgccgca aaaaagtaga
      661 ccaccggcag gcggcctacc cgaaaccatc cgactatctt ttggcatagt caggtggcgc
      721 taccgcacca atcaagcggt caattcatcg aaagcatcga cgattccgtt atggagataa
      781 ccgaagttat cgtaggcgtc ggccggcttc accgctcctc ctgcaaaaag tcgccgcccg
      841 ttttccaggt gaccctgcag gtactggctc cagtactgcc ccgcttcaga cgagttgtac
      901 tcgctcgcca gcacttgctg gaactgagcg gcagcgtcga tgaaacgccg catcggctcc
      961 agggcacaag ccggaacaca atcgggacca ggggccaaat cacgcacctg acgcgccccg
     1021 gccaggaaac cctcgtaggc accaaagatg cgtaaatgcc gtgcatctga cttgctatct
     1081 ctcatggttt ctcctgcaga cagagtccta tttgaaatgg cgggaagcgg cgcactccag
     1141 cttcagcgct tgcccttgcc cagctcccgc tcggcggcac gtgcccggtc gtggtactcc
     1201 acggcagcag ccagagcatc ggcagcttgc ttttccagca ggtcggccat ggtggcgtcg
     1261 cccagaactc gggtgcaagc agcctccgcc gaaagagcaa aggctaccgc gtccatcacg
     1321 cgccagaggc ggaagttgaa aatggccttg gtgggatagg cgttctcgtg atgctgcaca
     1381 tccaccttca gcccgatggc ttcgcgatgc acgctgcgcg cttcctcgca gagcagaatg
     1441 gcagtcgctt tcgcgtcctc accgtgggcg agttcccggg cctgcttcag cgcccgttca
     1501 ccacggtcgt agatggcctt ggtggcggtg tgcaggttgg cgatggtcat atcatccatg
     1561 aagtaactcc aaatctagcc ccgaagggct tctctaacgc taaaccctga gaaggcgcct
     1621 cgtgagcaca ttcacaggaa cgatttacat gccgccttgc cgggggtaat ggcttcgaca
     1681 gcaagactcc tgcttttaca aaaacaccag tcgctcaagc cggttcgaaa cccacgacac
     1741 ggatagtgcc ggggatgttc tactgatgct ggggttgcct gtgggggtag ctttttgcgc
     1801 ctgatgaaaa taaaagagaa agtaagcaac tgttagcaag aaaaagt
//
LOCUS       DAOQNZ010000905         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44625_length_1847_cov_2.998326, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000905 DAOQNZ010000000
VERSION     DAOQNZ010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44625_length_1847_cov_2.998326"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKW73_14710"
     CDS             <1..479
                     /locus_tag="PKW73_14710"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA74595.1"
                     /translation="VGASTMVVYTTFGSKEGLLNELWREGFRRLWETEEEALKQADPL
                     ARLHALGKAYRQNAITNPYFYKLVFGGQLPTEQLSKEMESDECNRTFTVLVQAVADCQ
                     KAGLIAGDLPDIDVAAMFWSTAHGAISLELSGMLGKWACAETIYTLNFENLLRGLK"
     gene            482..>1847
                     /locus_tag="PKW73_14715"
     CDS             482..>1847
                     /locus_tag="PKW73_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA74596.1"
                     /translation="MSFDYDAIIIGSGMGGLATGSLLAQLKGFKVLVLEKHFKVGGFT
                     HTFSRHGYKFDVGVHYVGEMQPGSRSQKLFDLVSGAAVEWAPMPDIYDKFVYPDFTVC
                     ASSNRAVYINELVNRFPHERSAIHKYFKDIDAVNLSIQTKTTASLLPGVLAAPVNAFN
                     SAFNKLAIISTADYLNANFKDNKLKAVLVSQWGDYGLPPSLSSFAIHSLIATHFLNGA
                     FYPTGSAETIAQAIVPIIKGAGGDVLVNHGVEEILVKNGTACGVTGKDQSGTTFRFTA
                     RHIISNAGIYPTFQKLLPENYKLAVPGFTEDLDASSISIYIGFKRSPKELGFQGENHW
                     LHSSYDHDAEYAARNNPEGKPGFAYLSFASLKDRLAEKHTAQVITIASAAPFKQWQNT
                     AWKKRGSEYEQYKERLTENLLAMIESRYAGFRDLVDYCELSTPLTVESFTGHPGGRIY
                     GQPMT"
BASE COUNT          482 a          534 c          440 g          391 t
ORIGIN      
        1 aagtgggagc ctcgaccatg gtggtctaca ccactttcgg cagcaaggaa ggcttactaa
       61 atgaactctg gcgggagggc ttccgcaggc tctgggaaac ggaagaagaa gctctcaaac
      121 aagccgatcc cctggccagg ctgcacgccc tcggtaaggc ttaccggcaa aacgcaatca
      181 ccaatcccta cttttataag cttgtttttg gcgggcaatt acccacagaa caattatcta
      241 aagagatgga aagcgatgaa tgcaaccgca ccttcacggt actggtgcaa gcggtagccg
      301 actgccagaa agccggtcta atagccggag atctgccaga catcgacgtg gcggccatgt
      361 tctggagcac cgctcatggt gccatcagcc tggagttgag cggtatgctc ggcaagtggg
      421 cttgtgccga aacaatttac actttgaact tcgaaaactt actaagaggt ctaaaataga
      481 aatgtctttt gactacgatg caatcataat cggctccggc atggggggct tagccacggg
      541 cagcctgctg gcgcaactga agggcttcaa ggtgctcgta ctggaaaagc atttcaaagt
      601 aggcggcttc acccatacct tcagccgcca tggctataag ttcgacgtgg gcgtgcacta
      661 cgtgggagaa atgcagcccg gcagccgctc tcaaaagctt tttgatttag tcagtggtgc
      721 cgccgtagag tgggcaccaa tgcctgatat ctacgataaa tttgtctacc ctgacttcac
      781 cgtttgcgct tcatccaatc gagcagtcta tatcaatgag ctggtgaacc gctttcccca
      841 cgagagaagc gccatccaca aatacttcaa agacatagac gccgtcaatc tctccatcca
      901 gaccaaaacc acggcaagcc tcctgccagg agtacttgcc gccccagtca atgctttcaa
      961 cagcgctttc aataaactgg cgattatatc tactgccgac tatttgaacg ccaacttcaa
     1021 agacaacaaa ctaaaagccg tgctggtttc ccagtggggt gactatggac taccgccatc
     1081 tctcagttct ttcgccatac actccttgat tgccacccac ttcttgaatg gtgccttcta
     1141 tcccaccggc tcggcagaaa ctatcgccca ggccatcgtg ccaattatca aaggagccgg
     1201 tggagacgtg ctggtcaatc acggggtgga agagatactg gtcaagaacg gcaccgcctg
     1261 cggcgtcacc ggtaaagatc agagcggcac caccttcaga tttacggccc gccatatcat
     1321 ttccaatgcc ggtatttatc ccactttcca aaaactgctg cccgaaaatt acaaactggc
     1381 agtaccaggc ttcaccgaag atcttgatgc ctcctccatt tccatttaca taggctttaa
     1441 acgcagccct aaagagcttg gcttccaggg cgagaaccac tggctgcatt caagctacga
     1501 tcacgacgcc gaatacgccg cccgcaataa tccggaaggc aagccaggct tcgcctatct
     1561 ttccttcgcc tcgctgaaag accgattggc cgaaaaacat accgcccagg ttatcaccat
     1621 agccagcgcc gcccccttca agcagtggca aaacaccgcc tggaaaaagc gcggcagtga
     1681 atacgagcaa tacaaggaac gcctgacgga aaatcttctt gccatgatag aaagccgtta
     1741 tgccggcttc agagatctgg tcgactactg cgagctttcc acccccctca ccgtggaaag
     1801 tttcaccggg cacccgggcg gccgcatata cggacaacca atgacac
//
LOCUS       DAOQNZ010000906         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_44865_length_1842_cov_2.635702, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000906 DAOQNZ010000000
VERSION     DAOQNZ010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44865_length_1842_cov_2.635702"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PKW73_14720"
     CDS             complement(<1..607)
                     /locus_tag="PKW73_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74597.1"
                     /translation="MAIQLNERDQLIFKLIDEHEVLLEKHISWFIAGDEKPVLIRDRL
                     RKLFYLDFLLCQRHGSKLPWWTTPTKPLVYMLSPMARKMHGFGENQEDVLDSDFQRHH
                     LEIANLRMLFLVAQKNGIISDLTWTTFKRERQVETGLNARVTFKHGGETKSLGLVNNP
                     EVSDIAVQHLLKSQLAEKLDWTLVVTREEADQKAVKASLAAL"
     gene            complement(685..1407)
                     /locus_tag="PKW73_14725"
     CDS             complement(685..1407)
                     /locus_tag="PKW73_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74598.1"
                     /translation="MEKVGKTRKGMRRQVLALLCLLVASFHGSLKAEAAPANEMFSSM
                     PKGGTKPVKKNTYVAPPPVQPANAVTAPGALSMTSAASPELTFFESLDTLCFQGWPRE
                     QERFYLSQTFDGELERVERWTKTARTVAKRYRNTATALRNLQIPNGRVDLQEYKEMRA
                     EWFDSAARVYEEMIKPKPPAQTMEELEAQLRKVDQDAEAVKHSKTTILAMDRRLRRLY
                     RVHEPRESDALQNYVMGVPKQK"
     gene            complement(1431..>1842)
                     /locus_tag="PKW73_14730"
     CDS             complement(1431..>1842)
                     /locus_tag="PKW73_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74599.1"
                     /translation="RPLPGREAIYAFPGVIPTVATFGRYLVILGVVSATVFMALAAYS
                     MVNGSPYGGARVLGAASGLFLLLASYTIWKIVQMNTFNANSDRAAQIQAKAGGAQVQD
                     AFMTRPNLPATPAVGATTTGRSGVPVQPLFNSGN"
BASE COUNT          378 a          478 c          525 g          461 t
ORIGIN      
        1 caagggctgc caggcttgcc tttacagcct tctggtcggc ttcttcccgg gttacgacca
       61 gggtccagtc cagtttctcg gcgagctgcg acttcaagag atgttggacc gcaatgtcac
      121 taacttcggg attgttaacc agccctaagc ttttggtctc accgccgtgt ttaaacgtca
      181 ctcgggcgtt taagccggtc tcgacctgac gctctctttt gaaggtggtc caggtcaggt
      241 cggagatgat gccgttcttc tgggctacca gaaaaagcat gcgcaagttg gctatctcca
      301 gatgatgtct ctggaagtcg ctatcgagaa cgtcctcctg attttcacca aagccgtgca
      361 ttttgcgagc catgggagag agcatgtaga ccagcggctt ggtgggtgtg gtccaccagg
      421 gcagtttgct gccatgtctc tggcaaagca gaaagtccag gtaaaaaagc ttccggagtc
      481 ggtctctgat caagaccggc ttttcatcgc cggctatgaa ccaggaaatg tgtttttcca
      541 ggagtacttc gtgctcatcg atcagtttga agatcagttg atcccgctcg tttaattgaa
      601 tcgccatttt gtcgtgtgcc tcaagggggg tgtttcggta agcgcgaatt ggaaaaagaa
      661 aactaggaaa gtgaggttgc ttacttactt ctgtttaggc actcccatta cataattttg
      721 tagagcatcg gattcgcgcg gctcatgcac gcgatagagt cttctcaagc gacggtccat
      781 ggctagaatg gttgttttgg aatgtttgac cgcttctgcg tcctgatcta ctttgcgcag
      841 ctgggcttcc agttcttcca tggtctgggc cggtggtttt ggtttaatca tctcctcgta
      901 gacccgggcg gcgctatcga accattccgc cctcatttcc ttgtattctt gcagatcaac
      961 cctgccgttg ggaatctgga gattacgtag tgccgtggca gtattacgat aacgtttggc
     1021 cacggtacgg gccgtcttgg tccagcgttc tactctttcc aactcgccgt caaaagtttg
     1081 agagagatag aagcgctctt gctctctggg ccaaccctga aaacacaagg tatcgaggga
     1141 ctcaaaaaaa gtcagctcgg gagaggctgc cgacgtcatg gaaagggcgc ccggagccgt
     1201 cacggcgtta gccggctgca ccggcggcgg tgctacatat gtatttttct tgacgggctt
     1261 ggtgccgccc ttgggcatgg aactaaacat ttcgttggcg ggagcggcct ctgcctttaa
     1321 agacccgtgg aagctcgcaa caagcaggca aagcagcgcg agaacctgcc gcctcattcc
     1381 tttcctcgtt tttccgactt tctccatggt cttttgctct cgtctattgc ttagttgcct
     1441 gaattaaaga ggggttgtac gggaacgcca gaccggcccg tggtagtggc acccacagcc
     1501 ggtgttgccg gcagattcgg tctggtcatg aaggcgtcct gaacctgggc gccgccggct
     1561 ttagcctgaa tctgggcggc gcggtcggag ttggcgttaa aagtattcat ctgtactatt
     1621 ttccagatcg tgtacgaggc cagtagcagg aagagacccg aggctgctcc cagaacgcgg
     1681 gcgccgccgt agggactgcc gtttaccatg gaataggcgg ccagagccat aaacaccgta
     1741 gctgatacca cgcccagaat gaccaggtag cggccgaagg tggccactgt cggaatcacg
     1801 ccgggaaagg cgtaaatggc ttcccgaccg ggcagaggcc tg
//
LOCUS       DAOQNZ010000907         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45082_length_1837_cov_4.506173, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000907 DAOQNZ010000000
VERSION     DAOQNZ010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45082_length_1837_cov_4.506173"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1837
                     /locus_tag="PKW73_14735"
     CDS             <1..>1837
                     /locus_tag="PKW73_14735"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNA74600.1"
                     /translation="YAVEAGLDMAIVNAQKILPLYRIDEEEREYHRKLVFDERIAAGA
                     DGTGGYDPLFALLDFYQKKQKSDKKVVRSVNLSIEEKLKQRVIDGDRVGIDIDLAKAL
                     ETYKPLDIINNILLDGMKVVGELFGSGKMQLPFVLQSAETMKSAVSYLEPFMERVEGS
                     ARGTMVLATVKGDVHDIGKNLVDIILTNNGYKVHNLGIKQSIENIAAAALEHNADCIG
                     MSGLLVKSTVIMKENLEILNQRGITTPVILGGAALTRRYVEEDCRRVYGGKLFYGQDA
                     FDDLHIMEAIASGREDELLRGLEAEPTDQCEDDDDDFGDEQAEGENNGKNSEVSHHLP
                     LKLRSDVERALPVCLPPFLGRKVLTDFDLDEIFSFINENSLIRGQFRVRQGDMPDAQY
                     EAMLAEKVYPALNALKERCRKEKLLAPAAVYGYYPCLSRGNELLVYEPDPLLSPAEVI
                     AGKRRDGSPIELKHKLSFSFPRQDFGRHLCIADFFRDEEEGGYDILPLQVVTMGQIAS
                     EHTARLFSAGEYTEYLYFHGLSVEAAEGLAEVVHKRVRAELGLASYDATDRRKFFQQG
                     YRGTRFSFGYPACPNLEDQSGLFQLLKPEEIGMELTEEFQLVPEQS"
BASE COUNT          431 a          457 c          506 g          443 t
ORIGIN      
        1 ctatgccgtg gaagccggcc ttgatatggc tatcgtcaat gcccagaaaa tcttgcccct
       61 ctaccgcatc gacgaagaag aaagagagta tcaccgcaaa cttgtctttg atgagcgtat
      121 agccgctggt gctgacggca ccggtggtta tgatccgcta ttcgcccttc tagacttcta
      181 tcagaagaag cagaagtctg acaaaaaggt ggtgcgaagc gtaaacctct ctatcgagga
      241 gaagctcaaa caacgagtca ttgacggcga ccgggttggt atcgacattg atctggcaaa
      301 ggcccttgag acttacaagc ctctcgatat tatcaataac atcttgcttg atggcatgaa
      361 agtggtgggc gaactctttg gttccggcaa gatgcaattg ccctttgtct tgcagtcagc
      421 tgaaaccatg aagtcggctg tttcctacct ggagcctttt atggagaggg tagagggctc
      481 ggcccgggga acgatggtat tggccacggt taagggcgat gtgcacgata tcggcaagaa
      541 cctggtggat atcattctca ccaataacgg ctacaaagta cacaatctgg gtatcaaaca
      601 atccatagaa aacattgccg ccgccgccct tgagcataat gcagattgca ttggcatgag
      661 cgggcttctg gtcaagtcta ccgtgatcat gaaggagaat ctggagattc tcaatcaaag
      721 aggcattacc acaccggtta tattgggtgg ggctgctctc acaaggcgtt atgtggaaga
      781 agactgtcgc cgggtttatg gcggcaagtt gttttatggt caggatgcct tcgacgacct
      841 gcacattatg gaagccatag cctccggtcg tgaagacgag cttcttcgcg ggcttgaggc
      901 agagccgacc gatcaatgcg aagatgacga tgacgatttc ggtgatgagc aagccgaagg
      961 cgagaataac ggcaagaact ctgaagtctc ccaccatctt ccgctcaagc tacgttccga
     1021 tgtggaaaga gcgctcccgg tctgcctgcc gcctttcttg ggtcgcaagg tactcaccga
     1081 tttcgatctg gatgagatct tctctttcat caacgaaaat tccctgattc gcgggcagtt
     1141 tagagttcga cagggcgata tgcccgatgc tcaatatgag gctatgcttg ccgagaaagt
     1201 ttatccggct ttgaatgccc tcaaggagcg ctgccggaaa gagaaacttc ttgctccggc
     1261 cgcggtttat ggttattacc cctgcctctc tcgcggcaat gaacttttgg tttacgagcc
     1321 ggaccctttg ctgagccccg ctgaagtcat tgcaggcaag cgccgcgacg gcagccccat
     1381 tgagctcaag cacaaacttt ccttttcctt cccgcggcag gacttcgggc gtcatctttg
     1441 catagccgac ttctttagag acgaggaaga aggcggctat gacatactgc ctctgcaggt
     1501 tgtgaccatg gggcagattg ccagtgagca tacggccaga ctctttagcg ccggtgaata
     1561 cactgagtat ctttatttcc acgggctttc agtggaggcg gcggagggac tggccgaggt
     1621 cgtgcacaag cgggtgcggg ctgagcttgg tcttgcttca tacgatgcta ccgaccggcg
     1681 caagtttttc cagcagggtt atcgcggcac gcgctttagt ttcggctatc cggcctgtcc
     1741 gaaccttgaa gatcaaagtg gtctctttca gttgctgaaa cccgaggaaa tcggcatgga
     1801 attgaccgag gaattccagc ttgtgcctga acaaagt
//
LOCUS       DAOQNZ010000908         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45083_length_1837_cov_4.491021, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000908 DAOQNZ010000000
VERSION     DAOQNZ010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45083_length_1837_cov_4.491021"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1312)
                     /locus_tag="PKW73_14740"
     CDS             complement(107..1312)
                     /locus_tag="PKW73_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNA74601.1"
                     /translation="MKNIGIITSGGDCGGLNGVIKGAALNALSKGMKAFIIPNGYAGL
                     YNLVDIKSLTELTRERLEEIEVIVAGSEAGNSRVKVSKIKDAGKYDRIKAGLKKFNLD
                     ALVIAGGDDTGSVVADLAGQGIPCVHAPKTMDLDLMPYSVGGDSTINRIAEAVRDLKT
                     TGRTHNRIIVMEVFGRYAGHTAFRGGVAADADAVLIPEVPVDFDVLYKHVKAAFIKRI
                     EESDVHAGTAMVVVAEGLKDASGNELVDMSSEPDSFGHRKLMGAGRYVVKQIEDRIKA
                     DPEVKEFMKRTGQFVEGVYEIPEVREIRPSHLVRCGFSSAVDSNFGLEAGAAAVELVS
                     QGTFGVTVVSLREGTLEYMDVKDAIVQRHVDLNDIALFETLGMSFGREPQKAKVQLQK
                     VTGKPKRVY"
     gene            1475..>1837
                     /locus_tag="PKW73_14745"
     CDS             1475..>1837
                     /locus_tag="PKW73_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74602.1"
                     /translation="MFQVLAKGRGLSGFAVQGLDLRQAEELLRAAEVKDAYFLGCLMS
                     QNLEHDMERRGACVFPALNGFAFKPFRPNLYTAAELYDKFDFEDPHTYDDCLDAVIMQ
                     QWRHLGGASPRGLINSLAQ"
BASE COUNT          400 a          506 c          450 g          481 t
ORIGIN      
        1 cccgccaggc aataaaaaag agaggaccgg cctctctttt ttactgattc aatcaagcat
       61 cggttatttg caagtagcgt tcatctgaat agaatcgttc tacctattaa taaacgcgct
      121 ttggtttgcc ggttactttc tgcaactgca ccttagcttt ctgcggctcc ctgccgaagc
      181 tcatgcccag ggtttcgaac aaggcgatgt cgttgagatc cacgtgtctt tgcacaatgg
      241 cgtctttaac atccatatac tccagggtgc cttctctcag gctcaccacc gtgacgccga
      301 aggtgccctg gctgaccagt tcaacagcag cggcgccggc ttccagtccg aaattggaat
      361 ctaccgccga gctgaaaccg cagcgcacca ggtgactggg gcgaatttcc cgtacttccg
      421 gaatctcgta gaccccttcc acaaactgtc ccgtgcgttt catgaactct ttcacttccg
      481 ggtcggcctt gatgcggtct tctatttgtt tgaccacata gcgtcctgcg cccatcagtt
      541 ttctatgacc gaaagaatcc ggctcggaac tcatgtctac cagctcgttg ccggaagcat
      601 ctttcaagcc ttccgccacc actaccatgg ctgtaccggc atgcacgtcg ctttcttcga
      661 tgcgcttgat gaaagctgct ttcacatgct tgtagagcac atcgaaatca accggtactt
      721 ccggaatcaa gacggcatca gcatcagcgg cgacaccgcc gcgaaaggcg gtgtgtccgg
      781 catagcggcc gaagacttcc ataacaatga tgcgattatg ggtgcgcccc gtggttttca
      841 gatctctaac tgcttctgca atgcgattga tggtggagtc gccgcccaca ctgtaaggca
      901 tcaaatccag gtccatggtc ttgggcgcat gcacgcaggg aattccctga ccggcaagat
      961 ctgccaccac cgagccggta tcgtcaccac cggcgatgac aagcgcgtcg aggttgaact
     1021 tcttcagtcc ggctttaata cgatcgtatt tgccggcatc tttgattttg gaaactttta
     1081 cccgtgaatt accggcttcg gagccggcca caatcacttc aatttcttcc agtctttctc
     1141 tggtcagctc ggtcaaactc ttgatatcga cgagattgta aagaccggcg tagccgttgg
     1201 gaataatgaa ggctttcatg cccttgctga gtgcgttcag ggcagcgccc ttgatgacac
     1261 cgttaagacc gccgcagtct ccgcccgatg taatgatgcc tatgtttttc acggttcctc
     1321 caggcttgct tggctcaaac aactgttatt gtttatcatg ctcaaaggtc tggaatactt
     1381 ccttatttaa ttcataatct atatgttaag cagaccagag acctcagtct tgtgggatac
     1441 ctgaagcgtg catgaaatag agtcacttga ggaattgttt caagtcctgg ctaaagggcg
     1501 tgggctttcc ggctttgccg tgcaggggct cgatcttcgt caggcggaag aactattgcg
     1561 ggctgccgag gtgaaagatg cttacttctt aggctgcctc atgagtcaga accttgaaca
     1621 cgatatggaa aggcgcggcg cttgcgtatt tccggctctt aatggtttcg cctttaagcc
     1681 cttccgcccc aatctctata cagcggcaga actgtacgac aaattcgact ttgaagatcc
     1741 ccacacctat gatgactgtc ttgatgcggt aattatgcag cagtggcgtc acctgggtgg
     1801 tgccagcccg cggggcttga tcaattcact ggcccag
//
LOCUS       DAOQNZ010000909         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45104_length_1837_cov_3.140853, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000909 DAOQNZ010000000
VERSION     DAOQNZ010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45104_length_1837_cov_3.140853"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1837)
                     /locus_tag="PKW73_14750"
     CDS             complement(<1..>1837)
                     /locus_tag="PKW73_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016636114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA74603.1"
                     /translation="LTDESKGQAGTVLKQNGLTRERILKVLTEIRGKQKVTSQDPEGT
                     YQALERYGKDLTEMAARGKLDPVIGRDDEIRRVMQVLSRRTKNNPVLVGEPGVGKTAI
                     AEGLAIRITKGDVPEGLKDKKLIALDMGSLVAGAKYRGEFEERLKAVLKEVTDAEGEI
                     LLFIDELHTVVGAGSGGEGSMDAGNLLKPPLARGELHCIGATTLDEYRKYVEKDPALE
                     RRFQTVFVDQPSVEETISILRGLKERYEVHHGVRIKDAALVSAAVLSDRYITDRALPD
                     KAIDLVDEAAARMRIEIDSMPTVLDELERRKIQLEIEREALKKEKDQASRERLEKLEK
                     ELANLKEESDNLRARWMAEKEGISKIQVIKADIETTKVQIEQAERATDLQKAAELKFG
                     KLIELEKQLKELEGQISGHKGPRLLKEEIDEEDIAEIIGSWTGIPVTKLLEGEVQKLL
                     RLEDHLHHRVIGQDEAIEVVANAVRRARTGMKDPKRPIGSFLFLGPTGVGKTELAKAL
                     AEFLFDDEQAIVRIDMSEYQEKHTVARLIGAPPGYIGHDDGGQLTEAVRRRSYSCVLF
                     DEVEKAHTDVFNILLQVLDEGHLTDSKGRTVDFKNTVIIMTSNIGS"
BASE COUNT          332 a          517 c          487 g          501 t
ORIGIN      
        1 ggctgccgat attggaggtc atgatgatga ccgtattttt gaagtctacg gtgcgacctt
       61 tggaatcggt caggtgacct tcatccagca cttgcaaaag gatgttgaag acatcggtat
      121 gggccttttc cacttcatcg aagagcacgc aactatagga gcggcgtctg accgcctcag
      181 ttagttgacc gccgtcgtcg tgaccgatat agccgggcgg agccccaatc aggcgagcaa
      241 cggtgtgctt ctcctgatat tccgacatat cgatgcgcac gatggcttgc tcgtcatcga
      301 agagaaattc agccagcgcc ttagccagct cggttttacc taccccggtg ggaccgagga
      361 agaggaagga gcctattggt cgtttgggat ctttcatgcc ggtgcgggca cggcgcacgg
      421 cgttggccac tacttcgatg gcttcgtcct gaccgattac gcggtggtgt aaatgatctt
      481 ccagacgaag caacttttgc acttcgcctt ccagcaattt ggtcaccgga atgccggtcc
      541 aactgccgat gatttcggca atgtcttctt cgtctatctc ttccttcaag agcctggggc
      601 ccttatgacc gcttatctga ccttccagtt ctttcagttg cttctccagt tcaattaact
      661 taccgaactt gagttccgct gctttctgta gatcggtggc tctttccgcc tgttcgattt
      721 gcaccttggt ggtttcgata tcggctttga tgacctgaat cttggagatg ccttctttct
      781 cggccatcca gcgtgctctc aaattgtccg actcttcctt gaggttggcc agttcctttt
      841 ccaacttctc caggcgctct ctggaagcct ggtctttctc ctttttgagg gcttctcttt
      901 caatctcaag ctgaattttc cgtctttcca gttcgtccaa taccgtcggc atagagtcga
      961 tttctatgcg catgcgggcc gccgcttcat caaccaggtc gatggctttg tcgggcaggg
     1021 cgcggtcggt aatatagcga tccgaaagca ccgcggccga gaccagagcg gcgtctttga
     1081 tacgcacgcc gtggtgcact tcatagcgct ccttgagacc gcgcagaata gatattgtct
     1141 cctctaccga aggctggtcc acaaagacgg tctggaagcg gcgttccaga gccggatctt
     1201 tttctacata tttacggtat tcatcaaggg tggtggcacc gatacaatgc agttcacccc
     1261 tggccagtgg tggcttcagc aagttgcctg cgtccatgga gccttcgccg ccggagccgg
     1321 cgcccaccac cgtgtgcaat tcgtcgatga agaggagaat ttccccttcg gcgtcggtga
     1381 cctctttcag aacagccttg aggcgctctt cgaattcacc gcgatatttg gcaccggcca
     1441 ccagtgagcc catgtcgaga gcaatcagtt ttttgtcttt caaaccttcc ggtacgtcac
     1501 ctttggtgat tctgatggcc agaccctcgg cgatggccgt tttgccgacg ccgggctcgc
     1561 ctaccagaac agggttgttc ttggtgcgac ggctcagtac ttgcattacc cggcgaattt
     1621 catcgtcccg gccgatgacg gggtcgagtt tgccgcgggc tgccatttct gtaagatctt
     1681 taccgtagcg ctccagggct tgataggttc cttccggatc ctggctggtc accttttgct
     1741 tgccgcgaat ttccgtcagc accttcaaga tgcgctcgcg ggtcaagccg ttttgcttga
     1801 ggacagtacc ggcctgacct ttgctttcgt cggtgag
//
LOCUS       DAOQNZ010000910         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45147_length_1836_cov_3.304323, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000910 DAOQNZ010000000
VERSION     DAOQNZ010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45147_length_1836_cov_3.304323"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..418)
                     /locus_tag="PKW73_14755"
     CDS             complement(92..418)
                     /locus_tag="PKW73_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74604.1"
                     /translation="MKATPHNHRSTWSVEPTLRGEPTMQTTHASTTGEKQSQKFTVEF
                     SSDAISALEEMKERLGKKSRADVLRSSLLLLKFLLDETQKGNQIAIISKGEGSNEKVK
                     ELALFV"
     gene            919..1563
                     /locus_tag="PKW73_14760"
     CDS             919..1563
                     /locus_tag="PKW73_14760"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNA74605.1"
                     /translation="MTRGPNIDQGELFEVADRLEAEGKEVTALAVQTALGRGSYTTVY
                     KYLEAWRASRPAPVAPVSFQMPDQVRNIFAGAWRMALMESAKETEAVKVKAAEDLKNA
                     AAQLKGALEAIEKLELAAEASATEIASLKESAQQKQTALSKAESELSARAAASEELRR
                     QLVDREGEIARLREELANGRTERDLAVREAAELKGKMTAIISQNDALLKRVGKR"
BASE COUNT          445 a          421 c          507 g          463 t
ORIGIN      
        1 gacttggacc ccaggagatt gcgtttccaa tccctggagc aatttcatga ggtccaattt
       61 tgtgatgtca tcgggcattt ttgcctccta attagacaaa caatgccagc tcttttacct
      121 tttcgtttga cccttcccct ttgctgatta tggcaatttg gttgcccttt tgagtttcgt
      181 ccaaaagaaa tttcaaaagc aaaagagagg atctgagaac gtcagcgcga gattttttgc
      241 ccaggcgttc cttcatctct tccaaagcgc tgatcgcatc ggaagaaaac tcaacggtaa
      301 atttctggct ttgcttttct cctgtagtgc ttgcgtgcgt ggtttgcata gtgggctctc
      361 ctctgagtgt cggttcgacg gaccaggtcg aacgatggtt gtgcggggtt gctttcaatc
      421 actcgccgag gagctatgtc caacacagtg gcgcagtttg agattaaaga ggcggttacc
      481 gaacaacggt tgttaacttg ggacaaggta cttcctcaac ggctcgcata gattcaaaag
      541 tgaaaagcgc agcatttagt tgattcgcag cttcctcgtc tcacagaagt gaatacactt
      601 agtccagata gcagtgcatc gcttctgtgt atattctacc cgatatgtgg tcgctgtcaa
      661 cacctaaagg gtgtaaatat aatgcagatt tgatgtagcg gctcggaccg gttgatgtcc
      721 acttgtggct cgatgctagg ctgcctgtct ttcgggagat gataaggaat gtggcgccgc
      781 cgcggggcgc ccacgaacgc taagcccata aaatgaggct tttggggctt tcttgttgct
      841 ttattggtaa taatagtagt aagaaaatgc aaactattat tatgctacaa tgctaaaagc
      901 accgcggagg gctcagcaat gaccagggga ccgaacatcg accagggcga actatttgaa
      961 gttgccgacc ggctagaagc tgagggtaaa gaggtaacgg ctttggcggt gcagactgcc
     1021 cttgggcgcg gcagctacac caccgtatac aagtacctgg aagcctggcg ggcttctcgt
     1081 ccggcaccgg tggcgccggt ctcctttcag atgccggacc aggtgaggaa catatttgcc
     1141 ggtgcctggc gtatggcatt aatggaatct gccaaggaaa ctgaagccgt gaaggttaag
     1201 gcggcagaag acctcaagaa tgctgccgct cagcttaagg gcgctctgga ggctattgag
     1261 aaactggagc tggcggcaga ggcttcggca actgaaattg cctcactgaa ggaaagcgcc
     1321 cagcaaaaac agacggcgct ttcgaaggct gaatctgaac tctctgcccg tgcggctgca
     1381 agcgaagaac tccgccgaca gcttgtggac cgtgagggcg agattgctcg cctgcgtgag
     1441 gaacttgcca acgggcgaac tgaaagagat ttggcagtga gagaggcggc tgaactcaaa
     1501 ggcaagatga ccgcaattat tagccagaac gatgctcttt tgaagcgcgt cggcaagaga
     1561 taatagggat gactttgttt tccctgagca tacaccggga accctagctt gacggcagct
     1621 tagtccttcg acagaacaat tcttctgttt atggcttgaa gctctctggt tggttgatct
     1681 agaagcttga tctcatttag cattgatgag ctaatggaga tgggttcctt tagaacaaac
     1741 caggagacaa cctcatcgtg ggagccggtg gtcaaagagc cggcggagca ttttttgttc
     1801 tgtaaattca gtcccggagc ggtttaatct ttctgg
//
LOCUS       DAOQNZ010000911         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45299_length_1833_cov_3.690664, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000911 DAOQNZ010000000
VERSION     DAOQNZ010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45299_length_1833_cov_3.690664"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="PKW73_14765"
     CDS             <1..1390
                     /locus_tag="PKW73_14765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HNA74606.1"
                     /translation="AGAGDLGYLLHKNPANLQTFPMSFGAAHVFYSEVGDARTTAVLM
                     LDVDPIALIRKSKSGAETSGFTLSQYVNDRPYCASSFLSVAIAQVFGSALAGRSRERQ
                     ELADQAICLEATVSVVWSHSGPAFIESLFTPLGYDVELSGAPLDAKFPEWGESPYYKL
                     KLRATTRLSDLLSHLYVLIPVLDNDKHYSIDKAEVEKLLRHGEGWLAAHPLKEVIVSR
                     YLRHSRRLTRSALEQLAEEAVDVEEEEVAAGEEEKLEERISLNTLRQQAVLDALKKCG
                     ARKVIDLGCGEGKLLKILLEDKGFEHVSGMDVSSRALSRLAKRLRLDETLQPSVPGRT
                     SRTGCTVFQGSLTYRDDRLQGYDAATCVEVIEHLDKDRLEAFARVVFAEAKPGVVVVT
                     TPNIEYNVRFEKLPAGKLRHSDHRFEFTRQEFKDWCESIALQHGYQVGFHPIGELDNE
                     VGAPTQMGVFTR"
     gene            1387..>1833
                     /locus_tag="PKW73_14770"
     CDS             1387..>1833
                     /locus_tag="PKW73_14770"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA74607.1"
                     /translation="MKIELPDLCLVLLIGASGSGKSTFARQHFKSTEIVSSDFCRGII
                     SDDENDQSCSAQAFALLHYIVDKRLAAGRLTVVEATNVQPDDRKALLRLAKENDCLGA
                     AIVLNMPENVCHERNASRPDRDFGPHVVRRHRTLLKKSLRFLKEEGF"
BASE COUNT          392 a          458 c          532 g          451 t
ORIGIN      
        1 ggcgggtgcg ggcgatctgg gttatttact gcataaaaac ccggccaatt tgcagacctt
       61 ccccatgagc tttggtgccg cccatgtgtt ctacagcgag gtcggcgacg cgcgtaccac
      121 cgcagtgctc atgctcgatg ttgacccgat cgccttgatt cgcaaaagca aaagcggggc
      181 tgaaacctcg gggtttaccc tttcgcaata tgtcaacgac cgcccctact gtgcctcctc
      241 tttcttgagt gtggctatcg cccaggtctt cggctcggct cttgccggtc gcagccggga
      301 gaggcaggaa ctggccgacc aggctatttg tctggaggct accgtctcag tggtatggag
      361 ccacagcgga cccgccttca tcgaatcgct atttacgccc ctgggttatg atgtggagct
      421 tagcggcgcc cccctcgatg cgaaatttcc cgagtggggc gagagcccgt actataagct
      481 taaactcagg gctaccactc gtctcagcga tttgctctcg cacctttatg tgcttatccc
      541 ggtgctcgat aacgacaagc actattctat cgataaggct gaagttgaaa agctcttgcg
      601 ccatggcgag ggctggttgg cggcgcatcc cctcaaggaa gtaattgtct ctcgctacct
      661 caggcattcc cggcgcttga ccagaagcgc tctggaacaa ctggcggaag aggcggtgga
      721 cgttgaagag gaagaagtcg ccgccggcga agaagagaag ttggaggagc gtatctcgct
      781 caacactttg aggcagcagg cggtgcttga tgctctcaaa aaatgcggtg cccgcaaagt
      841 tattgatttg ggttgtggtg agggcaaact gctcaagatt ctgctggaag ataaggggtt
      901 tgagcatgtc tcgggcatgg atgtgtccag tcgtgctctg agccgtcttg cgaagaggtt
      961 gcgtcttgat gaaaccttgc agccctctgt gcctggtcgt acaagtcgca ctggttgcac
     1021 ggtgtttcag ggatctctta cttaccggga cgatcgactg caaggctatg atgccgccac
     1081 ctgtgtggaa gtaattgagc accttgataa agaccggctg gaagcctttg cccgggtggt
     1141 ctttgccgag gctaagccgg gtgtggttgt tgtcactact cccaacattg agtacaacgt
     1201 gcgtttcgag aaattgccgg cgggtaaatt gcgccatagc gatcaccgct ttgaatttac
     1261 caggcaggaa ttcaaagatt ggtgcgaaag tattgccttg cagcatggct accaggttgg
     1321 ttttcatccc ataggagaac tggacaatga agtcggtgct cctacccaaa tgggggtatt
     1381 cacaagatga agatagaact gccagatttg tgcctggtgc tactcattgg tgcttccggc
     1441 tccggtaagt ctacttttgc caggcagcat tttaagagta ctgaaatagt ttcttcagac
     1501 ttttgtcggg gcatcatttc agatgatgaa aatgaccaga gttgttcggc gcaggccttt
     1561 gcgcttttgc actatatcgt tgataaacgc ctggcggcgg ggcgtttgac tgtggtggaa
     1621 gccaccaatg tgcagcctga cgacagaaaa gctctgctgc gcctggcaaa agaaaatgac
     1681 tgtctgggag cggcgattgt tctcaacatg cccgagaatg tctgccatga gcgtaatgct
     1741 tcgcgtccgg accgcgactt cggaccccac gtggtgcgtc gccatcggac acttttgaag
     1801 aagtcattgc gctttctgaa agaggaaggg ttt
//
LOCUS       DAOQNZ010000912         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45409_length_1831_cov_3.856419, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000912 DAOQNZ010000000
VERSION     DAOQNZ010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45409_length_1831_cov_3.856419"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PKW73_14775"
     CDS             complement(<1..972)
                     /locus_tag="PKW73_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA74608.1"
                     /translation="MKSELVNTEKYPVITAKGVSDPVALCASYLRTLILLAIGWLLAC
                     AVVHFAEVVQLILVLVCAWTHLATDPKMRLNTFRSKIDLKAKGLDIEDPYLNVLMPWE
                     MLSKVEIRQRRFNLIPNYVYFAFRNGQDVLILWDDVKETMDSTTLISCVRTWAPHAEV
                     VGDADLSKSESIATYTELWLREMSSEKCGARRRQDQTISVGTILNECYRVEKVLSGGG
                     QGTAYLSRVLPLPDLPSMPEQVVIKELVLPANAHGLKKATDSLVKEVAILRRINHPAI
                     IRLYDFFIEDMCGYLVIEYVDGVTLRRLVSQSGPLPESRVAAFGLTLC"
     gene            complement(1017..1553)
                     /locus_tag="PKW73_14780"
     CDS             complement(1017..1553)
                     /locus_tag="PKW73_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74609.1"
                     /translation="MPGKRGRDERQSSFSYDGPVRKIGASEQSPVAGVIFGAIFALVG
                     VSVVLLACGAFPVPESSFKAPHWVVGAVGGLFTLAGINVALQGLGVSPNSIVFRLLTP
                     VMLLCFGAPFAWVVFGDSHAPLEARILVGIFFGVFALVFGAAAVIGLNTGLMRRLARD
                     NPQLQERISELEKRKKGG"
     gene            complement(1570..>1831)
                     /locus_tag="PKW73_14785"
     CDS             complement(1570..>1831)
                     /locus_tag="PKW73_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74610.1"
                     /translation="KECACGAAGVYTAPVLVPALVELFEKHGALAKLERFTSEFGANF
                     YGLPLNEGMLILEEKHWTVPDEYHGVKPFLSGLNLKWSIKEN"
BASE COUNT          484 a          533 c          422 g          392 t
ORIGIN      
        1 gcaaagagta aggccaaagg ctgccactct tgattccgga agcggccccg actgacttac
       61 aagtcgccgg agcgtcactc cgtccacata ctctatgacc agatagccac acatatcttc
      121 tatgaaaaaa tcgtagaggc gaataatcgc tgggtgatta attctgcgaa gaatggcaac
      181 ttccttgacc agagagtcgg ttgccttttt cagtccatga gcatttgccg gtaagaccaa
      241 ttccttaatc acaacttgct ccggcatcga gggcaagtcc ggcaagggca aaactcggga
      301 cagataggcc gtgccctgtc cgccgccgct aagcactttc tcgacccggt agcattcatt
      361 caaaatcgtt cctacggaaa tcgtctggtc ttgccggcga cgtgccccgc atttttcgga
      421 actcatttct ctcagccata gttccgtata ggtggcgata gactccgatt tagataggtc
      481 tgcgtctccg acgacttcag catgcggagc ccaggtacga acgcaagaaa taagcgtggt
      541 ggagtccatg gtctccttca cgtcgtccca gagtattagc acatcctggc cgtttctgaa
      601 ggcgaagtat acgtaatttg gaatcaaatt aaagcggcgc tgtctgatct caaccttgct
      661 gagcatttcc cagggcataa ggacattcaa gtatggatct tctatgtcca gtcccttggc
      721 cttgaggtcg atcttcgaac gaaaggtatt cagcctcatc ttaggatctg ttgccaaatg
      781 agtccaggcg cagacaagta cgaggatcaa ttgcacaact tccgcaaaat gtacgaccgc
      841 acaagccaaa agccagccga ttgccagaag aatgagagtt ctcagataac tggcgcagag
      901 cgccaccgga tcggaaactc cctttgccgt aattacagga tacttttcgg tattaaccag
      961 ttcagacttc atgtagtctc gatttcaaca ccccattcgg aaaaacaaca ccatcactag
     1021 ccacccttct tacgtttctc cagttcgctg attcgctcct gcaattgcgg attatcccgt
     1081 gccagtcttc gcataagtcc ggtattcaaa ccgataactg ccgccgcacc aaatacaaga
     1141 gcaaagacgc cgaaaaagat gcccaccaga atgcgcgctt ccaggggagc atggctatca
     1201 ccgaagacaa cccaggcaaa cggcgccccg aaacaaagca gcatcacagg cgtaagcagc
     1261 ctgaaaacaa tggaattggg agaaactcca agcccctgca aagccacgtt gatgccggcc
     1321 agagtgaaga gcccgcccac cgcaccgacc acccagtggg gcgccttgaa ggaactctcc
     1381 ggtacaggaa aggcaccaca agcaagcaag actacgctca cccccaccaa ggcgaagatg
     1441 gcgccaaaaa tcacaccggc aaccgggctc tgctcactgg cgccaatctt tcttaccggt
     1501 ccgtcgtaag aaaaggaaga ttgtctctcg tctctcccgc gcttccccgg catttaatgc
     1561 ctcccacact tagttttcct tgatactcca cttcaggtta agccctgaga ggaacggctt
     1621 gacgccgtga tattcgtccg gcacagtcca gtgcttctct tccagaataa gcatgccctc
     1681 gttgaggggg agtccataga aatttgcccc aaattcggaa gtaaaccttt ccaacttagc
     1741 aagcgcaccg tgcttctcaa aaagttccac cagggccggc accaataccg gtgcggtata
     1801 gacaccggcg gcaccacaag cacactcttt a
//
LOCUS       DAOQNZ010000913         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45466_length_1830_cov_3.225915, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000913 DAOQNZ010000000
VERSION     DAOQNZ010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45466_length_1830_cov_3.225915"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..963
                     /locus_tag="PKW73_14790"
     CDS             361..963
                     /locus_tag="PKW73_14790"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA74611.1"
                     /translation="MQQLPPVTGVRPWTTLRREQIADCRVFSLHRQVSERESGKSANF
                     YVFHPRDWVNVIPVTRDMEVVLIEQYRHGIEKITLEIPGGMVDREDASTLVAAERELL
                     EETGFGGGETVFLGRNHPNPAIQSNICDTYLSLNVEYLQTPSFDTNEEVAIRMVPLKQ
                     VPELIADGTISHALVIVAFHYLQQLCLKDERYRHLRVYTY"
     gene            complement(960..>1830)
                     /locus_tag="PKW73_14795"
     CDS             complement(960..>1830)
                     /locus_tag="PKW73_14795"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HNA74612.1"
                     /translation="IRAIGRLSYLAVPLLLCGLAYAFVPLITPGKLMSLWQFKPSPSE
                     ALSFGATAATVAGGFIVGASLNPDYARFARTNKHAIAYALTDYAFAYPLLLLVAAVIA
                     VNFHSADIMIHLVPAGLTCLIFVMMMFATWAANDCNLYSSSLSLAAVFPKWKRAHLAI
                     LAGIFGISLAELHVAGHMVSFLTLLGIVIAPISGIFVINNLGRKHAVTEEELASIPDW
                     HSPTLIAWSGGVLLGFLATPREALGLGLLKLTTIPTIDAILGAATIMLIMKLVRSKRQ
                     CKSAAKVEPAVSV"
BASE COUNT          467 a          442 c          504 g          417 t
ORIGIN      
        1 gtcagtgagt ttgtgccgat gagcatgctc aatgctctta ccgaagcaat catcacatat
       61 gccgatgatc aggctcgtcg tggatatatc ctcggccagg aagatctcgt cgccgaattg
      121 aaaaagaaag cgtccaaaat cgcttagctt tgaatattgt ctggttggtg caggctcttc
      181 gctggagcac tagagtctga tctatagaaa tcagcaagac aatcgatata ggtttccgtc
      241 ccaccgaaat ccaacattcg cccgtctcat gcaaattgag gcgggcgttt tgcttagtat
      301 tttgtgacaa atttcgctat aatcaagccg tgcacggctg ataaaaacaa gggggctaag
      361 ttgcagcaat taccgccggt aactggtgtg agaccctgga ccaccttgag gcgcgagcaa
      421 atagccgact gtagagtctt ttccctgcat cgccaggttt ccgaacgaga aagcggcaag
      481 tccgccaatt tttatgtctt ccatcctcgt gattgggtca atgtcattcc cgtcaccaga
      541 gatatggaag tggtgcttat cgagcaatat cgtcatggca tagaaaagat caccctggaa
      601 attccaggcg ggatggttga tagggaggat gcttccactc tggtggcggc agaaagagag
      661 cttctggaag aaaccggatt tggtggcggc gaaacggtct ttctcgggcg aaaccatccc
      721 aatccggcta tccagagtaa tatttgcgat acctatttgt ctttgaatgt cgagtatttg
      781 cagactccga gttttgatac caatgaggaa gtagcaatcc gcatggtgcc tctcaagcag
      841 gtgccggagt taatcgccga cggcaccatc agtcatgccc tcgtcatcgt tgcattccac
      901 tacctgcagc aactctgcct caaggatgag cgttatcgtc atctgcgggt ctatacctat
      961 taaacagaga cggccggctc cacttttgcc gccgacttac actgacgttt agagcgcacc
     1021 agcttcataa taagcataat ggtcgcagca ccaaggatgg catcgatggt gggaattgtg
     1081 gtcagtttta gcagtcccaa tcccagcgcc tctctgggcg ttgccagaaa gcccagcaag
     1141 acaccgccac tccaggctat gagagtaggg ctatgccaat cgggtatgct tgccaattct
     1201 tcttcggtaa ctgcatgctt gcgtccgaga ttgttgatta cgaaaattcc tgagatagga
     1261 gcaatgacaa taccaagcaa ggtcaagaag gaaaccatgt gaccggccac gtgcagttcc
     1321 gccagactga tgccgaagat gccggccagg attgccaggt gggctctctt ccactttgga
     1381 aagacggcag ccaggctcaa ggaagatgag tagagattgc agtcattggc ggcccaggtg
     1441 gcgaacatca tcatgacgaa gatcaggcag gtgagaccgg ccggcaccaa gtggatcatg
     1501 atgtcggcgg aatgaaagtt gacggcaatc actgccgcta ccagcagcaa gaggggataa
     1561 gcaaaagcat agtccgtgag agcataggct atggcgtgtt tattggtgcg ggcgaagcgc
     1621 gcataatcag ggttgagtga ggcgcccact atgaagccac cggcaacggt ggcagcggtg
     1681 gcaccaaaac taagggcttc ggatgggctt ggtttgaatt gccagagtga catcagtttg
     1741 cccggtgtga tcagcggcac gaaggcatag gccagtccac aaagtaaaag tggcacggcc
     1801 aggtaactga gtcgtccgat tgctctgatg
//
LOCUS       DAOQNZ010000914         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45512_length_1829_cov_4.214205, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000914 DAOQNZ010000000
VERSION     DAOQNZ010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45512_length_1829_cov_4.214205"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..1115)
                     /locus_tag="PKW73_14800"
     CDS             complement(228..1115)
                     /locus_tag="PKW73_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA74613.1"
                     /translation="MTDAQIKSQEKVKLQLFSLDLSYQKVRQGTAKQGMESKSALPLA
                     VHDVSFSLFEGEILAIMGANGCGKSTLLKGMARALKPNSGKVEFEGENIWALSPKQFA
                     SKVAYLPQSLQIPDDMKVIELINLGRNPHQKWWQPSSHMDGKERAHLAEVIEKLDLGD
                     LKERNLAHLSGGERQRAIIAMALCQRPSLLLMDEPLSGLDFRHQLEILAYLKDLRRDG
                     LTIVLVLHDLNFAAHIANRVALLQKPTEASNSLAALGVPSEVLGSNVSKSVFQVDIDC
                     IETGDGRTVFYLKQEKENS"
     gene            complement(1112..>1829)
                     /locus_tag="PKW73_14805"
     CDS             complement(1112..>1829)
                     /locus_tag="PKW73_14805"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNA74614.1"
                     /translation="LGVAAAILIGLDLSLVPLIALMGGAATSLLVIVLSSRGGGSTAS
                     VSIPRLLLSGIALSSIASAAINLVMPVFGTQTMAQGLSLWLLGGLSGRNWSELLPAAC
                     YTLPALAVALMSSKQLRLLSLGEETAASLGLDVRKSQLVILSSAITLTAGAVSLSGLV
                     GFVGLIGPHLARRFVPQGERLQLAASALCGCILTLAADLGARIILPGQELPLGSLMAV
                     AGGPIFIYLLSKSLDQVVER"
BASE COUNT          394 a          508 c          508 g          419 t
ORIGIN      
        1 cagcagagct ggaacgaccc tcgcttctac gaccccggct atggcggcta ctaccagcag
       61 cagcgccgct attacggcaa taaccgcccc ggtataactt tccgcatcgg ttaagcggtt
      121 gaaagcagaa tttaacgtgg actgagtcac tcggtccacg tttttgcatg ggggcttatg
      181 gtttgttgct tgcactgaac tggcgcgaag cgtcagaagg gatgattcta gctattctct
      241 ttttcctgct tcaggtagaa aacggttcta ccatcaccgg tttcaataca gtcaatgtcc
      301 acctgaaaga ccgacttgct tacgttgctc ccgagcactt ccgacggtac ccctagagcc
      361 gccagactgt tcgacgcttc cgtcggtttt tgcaagagtg ccactctatt ggcgatatgg
      421 gcggcgaagt tgaggtcgtg caaaaccagg actatggtca gaccatccct gcgcaggtct
      481 ttcaagtaag cgaggatttc taattggtga cggaagtcca gccctgaaag aggttcgtcc
      541 attagcaaga ggctggggcg ctggcagagg gccatggcga tgatggccct ctgccgttct
      601 ccgcctgaca ggtgggctag atttctctct ttcaggtcgc ccaggtccag tttctctata
      661 acttccgcca gatgggccct ctctttgccg tccatgtgac tggagggctg ccaccacttc
      721 tggtgcgggt tgcgtcccag atttatcagt tcaatgacct tcatatcatc gggtatctga
      781 aggctctgcg gcaggtaggc tacttttgag gcgaactgtt tgggcgatag agcccagata
      841 ttttcacctt caaattccac tttaccgctg tttggtttaa gagccctggc catgcctttc
      901 agaagcgtgg acttgccgca gccgttcgct cccatgattg ccagtatctc tccctcaaac
      961 aggctgaaac taacgtcgtg caccgccagg ggaagagccg atttgctctc catgccctgt
     1021 tttgctgtgc cttgcctgac cttctgatag ctcaggtcca gcgaaaacag ttgcagcttc
     1081 actttctcct gggattttat ttgtgcgtct gtcatcgctc caccacctgg tccaggctct
     1141 tagacagtag atagataaag atgggaccgc cggctacagc catgaggctg cccagtggta
     1201 gctcctgtcc gggtagaata atgcgtgcgc cgagatcggc agccagggtt aaaatgcagc
     1261 cacagagggc tgaggctgcc agttgcagcc tctctccctg cggcacaaaa cgcctggcca
     1321 ggtggggacc gatcaagccc acaaagccca ccagaccact gagggagact gccccggccg
     1381 tcaaagtaat ggctgacgag agaatcacca gttggctctt tctgacgtcg agaccgaggg
     1441 aggctgctgt ttcttctccc aggcttagta ggcgcaactg cttgctgctc atcagggcta
     1501 ccgccagagc aggtagggta tagcaggcgg cgggcaaaag ttccgaccaa ttccggccgg
     1561 agagaccacc cagtagccag aggctcaatc cctgagccat ggtctgagta ccgaagacag
     1621 gcatgactaa atttatggcg gcactggcaa tggatgaaag ggcgatgccc gaaagaagta
     1681 atctgggtat ggagactgag gctgtgctgc cgcctccccg actggagagg acgatgacta
     1741 gcaagctggt ggctgcaccg cccatgaggg caatcagggg taccagactt aaatccagcc
     1801 cgatgagaat ggccgctgcc actcccagg
//
LOCUS       DAOQNZ010000915         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45618_length_1827_cov_3.437359, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000915 DAOQNZ010000000
VERSION     DAOQNZ010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45618_length_1827_cov_3.437359"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKW73_14810"
     CDS             <1..316
                     /locus_tag="PKW73_14810"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNA74615.1"
                     /translation="TGAANITGGGWTNLLRLNKDCGFALKAMELSPVFEELARHVEPA
                     ELYRTFNMGMGFAVMVRSAEAARAAIDIFARAGFAASVQGQVKSGHGVEIDCGKSTIF
                     LE"
     gene            364..>1827
                     /gene="purH"
                     /locus_tag="PKW73_14815"
     CDS             364..>1827
                     /gene="purH"
                     /locus_tag="PKW73_14815"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNA74616.1"
                     /translation="MLTVLASVFDKTGLVELLKALSEVEQVKVIATGSTCGYLREHGF
                     DGVKVEDMTGFPEILDGRVKTLHPRVFAGVLARASEADRATLKEHNISEIDVVVVNLY
                     PFAEKMKLGLKLSELIEFIDIGGPSLLRAGSKNYDRVAVLSSPSQYQAFAEAVKAGKA
                     DKAYRLQLAAAVFAQTKEYDGLIEQVLSAAARGEEAEGAHFPAAVSLSLKQVSTLRYG
                     ENPHQAAAWYQPPRLAEGLEADSFPPFEQLAGKELSANNIVDCYALVRILRDIRKATS
                     SAAEAACIIKHNNPCGVAIGKTTEEAFDRAYGTDPLSAFGGIYGFSGNVDGALAAKIV
                     QGFVEIVAAPSFSEEALKTFASKKNMRVLKLKAGLLGEANTASAEANPIRIKDMGDFG
                     LILEKDVEKPVSVAEFETACGDGKLAVSLKDDLAFAWNVVKHLTSNAIVVVKDGNAVG
                     FGIGQTSRVASVKLALAQGGALCQGAIMASDAFFPNVD"
BASE COUNT          410 a          464 c          524 g          429 t
ORIGIN      
        1 gaccggtgct gctaatatca ccggcggtgg ctggaccaac ttgctcagac tcaataaaga
       61 ttgcggtttt gccctgaaag ccatggaact aagcccggtc tttgaagagc ttgccaggca
      121 tgtggagccg gcggagcttt accgtacttt caatatgggc atgggctttg ccgtcatggt
      181 caggagtgcg gaagcggccc gtgcggccat cgatattttt gccagagccg gttttgccgc
      241 ttccgtacag ggacaggtga aaagcgggca cggagtcgag atagactgcg gaaaatctac
      301 aatttttcta gagtgattgt tgcaaaatta gctaccagga aaactagcta ccagggggat
      361 gaaatgctga cagtattggc aagcgtattt gacaagaccg ggctcgtaga gcttctcaaa
      421 gctctttcgg aagttgagca ggtgaaagtg atagccacag gctccacttg cggctacctg
      481 cgcgagcatg gttttgacgg cgtcaaggtg gaagacatga cgggcttccc cgagattctt
      541 gatggacggg tcaagaccct tcatccccgg gtctttgccg gtgtgctggc cagagcttcg
      601 gaggctgacc gggctacttt gaaagaacac aacatcagcg aaatcgatgt ggtagttgtg
      661 aatttgtatc cctttgctga gaaaatgaag ctcggactga agctttccga actgatagag
      721 ttcattgata tcggtggtcc cagcttgctg agagccggct ccaaaaacta tgaccgggtt
      781 gcggttttgt ctagccccag ccagtatcag gcttttgccg aggccgtcaa agccggcaag
      841 gccgataaag cctaccgctt gcagttggcc gccgccgtct ttgcccagac caaagaatac
      901 gacggcttga tagagcaagt gttgagtgcc gctgccagag gcgaagaagc cgagggcgct
      961 catttcccgg ctgccgtttc tttgagtctg aagcaggtga gcacccttcg ttatggtgaa
     1021 aacccgcatc aggctgcggc ctggtatcaa ccgccccgcc tggccgaagg tctggaagcc
     1081 gatagtttcc cgccctttga acagttggcc ggcaaagagc tttccgccaa caatattgtt
     1141 gactgctacg ccctggttcg cattttgcgc gatattcgca aagccacatc aagcgctgct
     1201 gaagctgcct gtatcatcaa gcacaataat ccctgcggcg tggctatcgg taagaccacg
     1261 gaagaagcct tcgaccgggc ttacggcacc gacccgcttt ccgctttcgg aggcatatat
     1321 ggcttttccg gcaatgtaga cggtgctctt gccgccaaga tcgttcaagg tttcgtggaa
     1381 atagtcgccg ctccctcttt cagtgaagaa gccctcaaga cctttgcctc gaaaaagaat
     1441 atgcgcgtgc tcaaactgaa ggccggtttg ctgggcgaag ccaataccgc ctctgcggaa
     1501 gccaatccga ttcgtattaa ggatatgggc gactttggtt tgatccttga gaaagatgtt
     1561 gaaaagccgg tatccgtagc cgagtttgaa accgcctgcg gtgacggaaa gcttgccgta
     1621 agccttaaag atgatctggc ttttgcctgg aatgtggtca agcatctgac ttccaatgcc
     1681 atcgtcgtgg taaaagacgg caacgccgtc ggcttcggta ttggtcagac cagccgggtc
     1741 gcttcggtga aactggctct ggctcagggt ggtgcactct gccagggggc gattatggcc
     1801 agtgatgctt tcttccccaa tgtggac
//
LOCUS       DAOQNZ010000916         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45634_length_1827_cov_2.742099, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000916 DAOQNZ010000000
VERSION     DAOQNZ010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45634_length_1827_cov_2.742099"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1243
                     /gene="gcvT"
                     /locus_tag="PKW73_14820"
     CDS             140..1243
                     /gene="gcvT"
                     /locus_tag="PKW73_14820"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNA74617.1"
                     /translation="MATTTLRETPLASAHRKLSARMVDFAGWNMPVQYKSIVTEHMAT
                     RERLGIFDVSHMGEFLVTGDKAADFVQYVIANDVNRLTEPNRALYSQLCYEDGGTVDD
                     LIVYRRKDNFMLVVNASNIEKDFAWLKSHAGKFGVTLTDLSDDTGLIALQGPQAVPLL
                     AEIAGDFVSSLPSFGYGEATVDGVSFSFGRTGYTGEDGFEIFVPAAKVEWLWNTLLER
                     GAGRGIEPCGLGARDTLRLEAGLPLYGHELEKDITPIESGLGWSVKPEKGDFIGREVL
                     AKQKAGELSRQIVCLKAEGKALPRQGYAVLHGEQEVGKITSGSQGIFVGYPIAFAMVP
                     KELTKVGTELSIAIRDTKVPAKVVTRPWYKRKK"
     gene            complement(1286..1411)
                     /locus_tag="PKW73_14825"
     CDS             complement(1286..1411)
                     /locus_tag="PKW73_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74618.1"
                     /translation="MNYRIHSIAQTRRMPAPGRTLDLIAFAAMCGAYLLLLTCVS"
     gene            1578..>1827
                     /locus_tag="PKW73_14830"
     CDS             1578..>1827
                     /locus_tag="PKW73_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74619.1"
                     /translation="MSDINTGQMMGLALGGVVVVMVFAGPMLIGRIFGSGKSGKSAEL
                     IPAAETYFLSCGESMTVVRQVLTRESFLGCRYRITYDRM"
BASE COUNT          411 a          432 c          539 g          445 t
ORIGIN      
        1 cggcggggcg gcggcggtgg acgccggcgt taattcccgt aggggagcat tatctagtca
       61 aggcgcgcct gcccagtagc aagtaaatag ctctcagggt ggtatctttt catcgatata
      121 tccactggga gataaggaaa tggctacaac caccctgaga gaaacaccgc tggcctcggc
      181 gcaccgcaaa ctgtcggcgc ggatggtgga tttcgccggt tggaacatgc cggtgcagta
      241 taagtcgatt gtcaccgagc atatggcaac gcgcgagcgt cttggcattt tcgatgtctc
      301 tcacatgggt gagtttctag tcaccggcga taaggccgcc gatttcgtgc aatacgtaat
      361 tgccaatgat gtaaatcgct tgaccgagcc caaccgcgct ctttattctc aactttgcta
      421 cgaagacggc ggtaccgtgg atgatttgat tgtctatcgc cgcaaagata attttatgtt
      481 ggtggtcaat gcgtccaaca tcgagaagga ttttgcctgg ctcaaaagcc atgccggaaa
      541 attcggtgtt accctcaccg acctttcgga cgacaccggt ctaattgccc tgcaaggtcc
      601 gcaggctgtg ccgcttttgg ctgaaattgc cggagacttc gtttcttccc tgccctcttt
      661 cggttatggt gaagccaccg tggatggtgt gagcttcagt ttcggtcgca ccggttatac
      721 cggtgaggac ggcttcgaga tttttgtgcc ggccgccaag gtagaatggc tctggaacac
      781 ccttctggaa agaggcgctg gtcgcggcat cgagccttgc ggtttgggtg cccgcgatac
      841 gttgcgcctg gaagccggtc ttccccttta cggccacgaa ttggagaaag atattacccc
      901 catcgagtcg gggcttggct ggagcgttaa gcctgagaag ggcgatttca ttggtcggga
      961 agtgctggct aagcagaaag ccggtgagct ttccaggcag attgtttgct tgaaagccga
     1021 aggcaaggcg ctgcctcgtc agggctatgc tgttttgcac ggagagcagg aagtgggcaa
     1081 gataaccagc ggctctcagg gaatatttgt tggctatcct attgcctttg ccatggttcc
     1141 caaagaattg accaaggtgg gaacggaact ttcaatcgct attcgtgata ccaaagtgcc
     1201 tgccaaagtt gtgacaagac cgtggtataa gcgcaaaaaa taaggatgga aaaggattcc
     1261 acggcactgg accgcactgg aagaattagc tgacgcatgt gagcaagagc aggtaggctc
     1321 cgcacatggc ggcgaaggcg atcagatcga gggtacggcc cggggcgggc attcttctgg
     1381 tctgggcgat ggaatgaatt ctgtagttca tggtaattgc tgctttctag attctcaaaa
     1441 ttgtctgtcg tttgcggcga caattagcta gactgtctgc ctggaagatt tagtaagaaa
     1501 aacgagagat tttcttacac acctcaaaat tgcaaccagg taaatagaac aagagtacgc
     1561 aggcgcagga ggacaggttg tccgatatca atacagggca gatgatgggc ttggcgcttg
     1621 gcggcgtggt cgttgtcatg gtgttcgccg gtccaatgct tatcggtcgt atttttggca
     1681 gcggtaaaag cggtaagagt gccgaactga ttccggctgc ggagacttat tttctctcct
     1741 gcggcgaatc aatgaccgtg gtgcgccagg tgctcacacg cgaatccttt ctaggctgcc
     1801 gttaccggat tacttacgat cgcatgg
//
LOCUS       DAOQNZ010000917         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45659_length_1826_cov_4.345567, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000917 DAOQNZ010000000
VERSION     DAOQNZ010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45659_length_1826_cov_4.345567"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1020
                     /gene="mdh"
                     /locus_tag="PKW73_14835"
     CDS             82..1020
                     /gene="mdh"
                     /locus_tag="PKW73_14835"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNA74620.1"
                     /translation="MSKVTVVGSGFVGATVAQLLADKDIADVCLVDVIDGMPQGKSLD
                     MMQAAAVHGFDRKLVGSNDYADTKGSDIVVITAGIARKPGMSRDDLLKTNANIVQTVA
                     RETVKHSPDAIFIVVTNPLDVMTYLTWKTTKLPASRVIGMAGVLDSARFQTFIAMETG
                     FSVQDVRAMVLGGHGDLMVPLTRCATVNGVPIANFLSKEKIDEMVKRTQNGGAEIVAL
                     LKSGSAYYAPGSSVVQMVEAILKDQNRLLPCAVLADGDYGLKDVYIGLPTVLGREGVK
                     RIVELKLEKEEAELLKKSADSIKENIDLMNKLLVAV"
     gene            1215..>1826
                     /locus_tag="PKW73_14840"
     CDS             1215..>1826
                     /locus_tag="PKW73_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA74621.1"
                     /translation="MTPDLISLLSRKYKAALFLALSCQIAVLSNGQASAVAQDGQVGK
                     GDKLAQASVSTAPTGDFSFDDDKSHETERKEVEEFLNNLETMWNRHDIDGVMGNYADD
                     YVNNDGIDKNTVRKLTSEFWKTYPDAHSSSATKNIRIDGLYATVDSRDQATGTTANEF
                     QSIKSKGELQSLSEGRLYLKKVSGHWKIIGDRIDFEKVKVSFGL"
BASE COUNT          462 a          514 c          456 g          394 t
ORIGIN      
        1 aaagacgggc gcttctttca tgttaacctg gctgttgttt aaatctcagt ccgcatgcgc
       61 ggacctgtgg aggtactttc cgtgagtaaa gtaacagtag ttggatccgg ttttgtgggc
      121 gccacagttg cccagctcct ggctgacaaa gacatcgccg atgtttgcct ggtagacgta
      181 atcgacggta tgccccaggg caagtccctt gacatgatgc aggctgccgc cgttcatggc
      241 ttcgaccgta agcttgtcgg ttcgaacgat tacgctgata ccaagggctc cgacatcgtc
      301 gtgatcactg ccggtatcgc cagaaaaccg ggcatgtcca gagacgacct gcttaagacc
      361 aatgccaaca ttgttcagac cgtcgcgaga gaaaccgtca agcactcacc cgatgccatt
      421 ttcatcgtgg tcaccaatcc gcttgacgtc atgacctacc tcacctggaa aaccaccaaa
      481 ttgcccgcct cccgcgtcat cggcatggcc ggcgtactgg actccgctcg cttccagacc
      541 ttcatcgcca tggaaaccgg tttctccgta caagacgtgc gcgccatggt tctgggcggt
      601 cacggcgacc tgatggtgcc cctgacccgc tgcgccaccg tcaacggcgt tccgatagcc
      661 aacttcctct ccaaagaaaa aatcgacgaa atggtcaagc gcacccagaa cggcggcgcc
      721 gaaatcgtag ccttgctcaa gtccggcagc gcctactacg cccccggttc ctcggtggta
      781 cagatggtgg aagccatcct caaagaccag aacagactgc tgccctgcgc cgtgcttgct
      841 gatggagact acggtctcaa ggacgtctac atcggcttgc ccacagttct gggcagagaa
      901 ggcgtcaagc gcattgtgga actgaaactg gagaaagaag aagctgaact cctgaagaaa
      961 tcagccgatt ccatcaaaga gaacatcgac ttgatgaaca agctcctggt tgcagtctaa
     1021 gccgcgagcg ccgggcccag caggctccac cttgtaattc gactaaagta gcccggattt
     1081 gcagcccatt gggggttagt ttaagcaaat ctgggctttt gcttgcttga catataaata
     1141 ggtataatat ccatgatttc ctaaaatcac tcctctttac tgcatactgc aattttcgcc
     1201 acggaaggtc caaggtgaca ccagatttga tatcactcct gagtcgcaaa tataaagccg
     1261 ctcttttcct cgcactctca tgtcagatcg ccgtcttatc aaacgggcag gcctcagccg
     1321 ttgcgcaaga cgggcaagtg ggcaaaggcg ataaactggc ccaggccagt gtgagcaccg
     1381 caccaaccgg tgattttagt ttcgatgatg ataagagcca cgagacggaa cgcaaggaag
     1441 tagaggagtt tctcaacaat cttgagacca tgtggaatcg tcacgacatc gacggcgtca
     1501 tgggcaacta tgcagatgac tacgtcaaca acgacggcat agataaaaac acagtgcgta
     1561 agttaacgag cgaattctgg aagacctatc cggacgctca ctcttcttcc gccaccaaaa
     1621 atattcgcat cgacggactt tacgccactg tagattcccg cgaccaggca accggcacca
     1681 ccgccaacga attccagtct atcaagagca aaggcgaatt gcagtcgctt tcggaaggtc
     1741 gcctctatct caaaaaagtc agcggtcact ggaaaataat tggcgaccgc atcgattttg
     1801 aaaaggtgaa agtctccttt ggcctg
//
LOCUS       DAOQNZ010000918         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45782_length_1824_cov_3.351611, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000918 DAOQNZ010000000
VERSION     DAOQNZ010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45782_length_1824_cov_3.351611"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /gene="gatA"
                     /locus_tag="PKW73_14845"
     CDS             complement(<1..1471)
                     /gene="gatA"
                     /locus_tag="PKW73_14845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNA74622.1"
                     /translation="MSKSQLIGRSIAELHSLIKAKETSCLEITEAHIEQIEKGDQELG
                     AFLSYTFDQARAAAASWDERLTKGEAMPLLAGVPVAIKDNMCEVDTRTTCGSKILENF
                     VSPYRAYAVEKLHDAGALILGKTNLDEFAMGSSTEHSAYKVTRNPIDPDYVPGGSSGG
                     SAAAVGAGFCVTALGSDTGGSIRQPASYCGLVGMKPTYGMVSRFGLVAFASSLDQIGP
                     FARNVEDCALTLLAIAGHDKRDSTCLSESYREQYAKPALTRDFIKEFKAGSPEENLKG
                     KRIGIIKELIGDGIDEDVKRPILKAAALLEKCGARVEEVSLPHAKYALSVYYLVATAE
                     ASANLARFDGVRYGHRNMDAAELHAMYYASRNEGFGAEVKRRIMLGTYALSSGYYDAY
                     YKKAQQVRALITADFHKIFENFEMVVSPTAPSAAFKFGAKSDDPIQMYMSDIASIPAN
                     LAGLPGISLPCGLADSNMPVGIQLESARPQGKEIPGRPAR"
     gene            complement(1505..>1824)
                     /locus_tag="PKW73_14850"
     CDS             complement(1505..>1824)
                     /locus_tag="PKW73_14850"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ACP synthase"
                     /protein_id="HNA74623.1"
                     /translation="ETNAIKAVFGEHATSHKLCVSSTKSMTGHLLGAAGAIEAAGAGL
                     AIEDSIIPPTINYEEPDPDCDLNYVPNKAIHGAKVEITMSNSFGFGGHNCSLVFRRFK
                     DAY"
BASE COUNT          431 a          473 c          498 g          422 t
ORIGIN      
        1 acctggccgg tctgccgggt atttccttgc cctgcggtct cgctgattcc aactgaatac
       61 cgaccggcat gttggaatca gcgagaccgc agggcaagga aatacccggc agaccggcca
      121 ggttggcggg tatggaagca atgtcggaca tgtacatctg aattggatcg tcggacttgg
      181 caccgaattt gaaggcggca ctgggtgccg tcggcgagac aaccatctca aagttttcga
      241 agattttatg gaaatcggct gtaataagcg ctcttacctg ctgtgccttt ttgtaataag
      301 catcgtaata gcctgaactc aaggcatagg taccaagcat aatgcggcgc ttcacctcgg
      361 cgccgaaacc ttcgttgcgg ctggcgtaat acatggcatg cagttccgcc gcatccatat
      421 tgcgatggcc gtaacgcaca ccatcaaagc gagctaaatt agcggaagcc tcggctgttg
      481 ccaccaggta ataaacggaa agggcatact tggcatgggg cagagaaact tcctctaccc
      541 gggcaccgca tttctcaagc agggctgccg cttttagaat agggcgcttc acgtcctcat
      601 cgatgccgtc accgattagt tctttgatga tgccgatgcg cttacccttg aggttttctt
      661 ccggcgaacc ggccttgaac tctttgataa agtctctggt aagagcaggt tttgcatact
      721 gctcacgata actctcagac agacatgtgg agtcgcgttt atcatggccg gcaatggcca
      781 gcaaggtgag ggcgcaatct tccacattgc gagcaaaggg gccaatttga tccaggctgg
      841 aggcgaaagc caccagacca aagcgagaaa ccatgccgta ggtgggtttc atacccacca
      901 gcccgcaata agaggcgggc tgtctgatgg agccgccggt atcggaacca agggcggtga
      961 cacagaagcc ggcgcccact gccgctgccg aaccgcctga actgccgcca ggcacatagt
     1021 caggatcgat agggtttctg gttaccttat aggccgaatg ctcagtggaa gagcccatgg
     1081 cgaactcatc caggttcgtc tttcccagta tcaaagcgcc tgcatcatgc agcttttcaa
     1141 cagcataagc ccggtacggg ctgacgaaat tctcaaggat tttgctaccg caggtagtgc
     1201 gagtatcaac ttcgcacata ttgtctttaa tggccaccgg cacaccggcc aataagggca
     1261 tggcctcacc tttagtcagt ctctcatccc agcttgcagc agcggctctg gcttgatcaa
     1321 acgtatagga gagaaaggcg cccagttctt ggtcgccttt ctcaatttgc tcgatatgag
     1381 cttcagtgat ttcaaggcaa gaagtttctt tcgccttgat gagcgaatgc aactcggcaa
     1441 tggagcgacc gattagttga gacttggaca taggggcaaa ggtccttttg taattcgaac
     1501 gtccttagta ggcgtctttg aatcgtctga aaacaagaga gcagttgtga ccgccgaaac
     1561 cgaagctgtt tgacatagtg atctcaacct tggctccgtg gatggcctta ttgggcacat
     1621 agttgagatc gcagtccgga tcgggctctt cgtagttgat ggtaggagga ataatagaat
     1681 cctcaattgc cagacccgct cccgctgctt cgatggctcc cgccgcaccg agcaggtgcc
     1741 cggtcataga cttggttgaa gagacacaga gcttatggga agtagcgtgc tcaccgaaga
     1801 ctgctttgat ggcattggtc tcgg
//
LOCUS       DAOQNZ010000919         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45884_length_1822_cov_3.314658, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000919 DAOQNZ010000000
VERSION     DAOQNZ010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45884_length_1822_cov_3.314658"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKW73_14855"
     CDS             complement(<1..523)
                     /locus_tag="PKW73_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74624.1"
                     /translation="MASALFDARLYRKDAAEPVSVKVQIESHRLRVLPQPDSLEPDLE
                     LDYGRAQVELAGQDKNRVKVQPLDASYALIFQEREVLASLALYAKGTALAKQADLLYQ
                     KAGLQKRGEKIYWTKVALAVVLISLIGYLSFGAIVDAAVERVDPSMEGKIGEFIANVN
                     KWQDSGADAERVKR"
     gene            complement(525..1211)
                     /locus_tag="PKW73_14860"
     CDS             complement(525..1211)
                     /locus_tag="PKW73_14860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNA74625.1"
                     /translation="MDYELLHEGSNAVLKVNLQRGEQIKAEAGAMVAKTGHLVIEGKL
                     WGGMVSALKRSVLGGETLFFQEVTAQEGPGEVLLAPSPPGDVKVIHISGGQDYYVKNG
                     CLLAAMDQIEMDTKAQRLSAGAFSGAGLFVLHLKGNGGFAVSAFGAIMEINVPPGEEY
                     MVDNGHLVAWSGDTSYRIVKAGKNWMSSITSGEGLGCMFTGPGRIYVQTRNPEAFGAW
                     VRRFVPATGG"
     gene            complement(1257..>1822)
                     /locus_tag="PKW73_14865"
     CDS             complement(1257..>1822)
                     /locus_tag="PKW73_14865"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNA74626.1"
                     /translation="AFVIYQVVEGETRDIFSQLGDVPPMPCAIVFGAGIASPEVRDRV
                     QTAVALYKEGKVKKLLMTGDNGHVNYNEPLAMRQEALELGVPAADVVCDYAGFRTYDS
                     LYRARDIFKVQKAVLVTQRYHLPRAMYLARRLGLTVVGIGAGRDSYGSIQTWYDLREI
                     GAAQAAWLDTTLGRKPKYLGKVEPIFP"
BASE COUNT          436 a          545 c          442 g          399 t
ORIGIN      
        1 cccgctttac tctctcggca tcggcgccac tatcctgcca cttgttgaca ttggcgatga
       61 actcaccaat cttgccttcc atactgggat caacacgctc aaccgccgca tcgacaatgg
      121 caccgaagct gaggtagccg atcaaagaaa tgagaacaac ggcaagagca acttttgtcc
      181 agtaaatttt ctcaccgcgc ttttgcagac ctgccttttg ataaagcagg tctgcttgct
      241 tagccagagc tgttcccttg gcgtaaagtg ccagggaagc cagaacttcg cgctcttgaa
      301 aaataagggc ataggaagca tcaagtggtt gcaccttgac ccgattcttg tcctgcccgg
      361 ccagttccac ctgagcccgc ccataatcca gttcaaggtc aggctccagg ctatcaggtt
      421 ggggaagcac cctgagccga tgggattcta tttgcacttt tacgcttacc ggctcggcgg
      481 catccttccg gtagagcctg gcatcaaaca aagccgaagc catgttagcc gccggtagcc
      541 ggtacaaaac gtctcaccca ggcaccaaag gcttccgggt tgcgggtttg cacataaatc
      601 cgaccgggac cggtaaacat acaaccgagc ccttcaccac tggtgatact ggacatccag
      661 ttcttgccgg ctttgacgat gcgataggaa gtatcgccgc tccaggccac cagatggccg
      721 ttgtctacca tgtactcttc gcccggaggc acattgattt ccataatggc gccgaaagca
      781 ctgacggcaa agccaccatt gcctttcaag tgcaatacaa ataatcccgc gccggaaaat
      841 gcaccggcgg aaaggcgctg agcctttgta tccatctcaa tttgatccat ggcggccagc
      901 aagcagccat ttttcacata atagtcttga ccaccgctaa tatgaatcac cttcacatca
      961 ccgggaggcg agggtgccaa aagcacctcg ccaggaccct cctgggcagt cacttcctga
     1021 aagaaaaggg tttcgccgcc cagcacactg cgcttgagcg cgctgaccat gccaccccag
     1081 agtttgcctt cgatcaccag atgtccggtc ttggccacca tggcaccggc ttcagcttta
     1141 atctgctcgc cccgttgcag attcactttc aaaacggcat tggatccctc gtgcagcagt
     1201 tcgtagtcca tatcactcag ccttattttg gaggaatcat agattttagc agttactcaa
     1261 gggaaaatag gttcgacctt gcccagatac ttaggtttac gacccagggt ggtgtccagc
     1321 caggccgcct gtgccgctcc tatttcacgc aggtcgtacc aggtttgaat ggagccatag
     1381 gaatctcgcc cggccccaat acccaccact gtaagtccaa ggcgacgagc cagatacatg
     1441 gctcggggca ggtgataccg ctgggtcacc aacacggctt tttgcacctt aaaaatgtcc
     1501 ctggcccggt aaagactgtc gtaggtgcga aaaccggcat agtcgcaaac cacatcggcg
     1561 gcaggcacac ccaattccag ggcttcctgc cgcatggcaa gcggctcatt gtaatttaca
     1621 tgaccattgt ccccggtcat gagcagtttc ttgactttgc cttctttata gagagcaaca
     1681 gcagtctgca cgcgatcacg cacttccggc gaagcaatgc ctgcgccaaa aacaatggcg
     1741 cagggcatgg gcggcacatc gcccaactgg ctgaagatat cccgggtctc gccttccact
     1801 acctggtaga taacaaaagc ag
//
LOCUS       DAOQNZ010000920         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45924_length_1821_cov_3.835221, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000920 DAOQNZ010000000
VERSION     DAOQNZ010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45924_length_1821_cov_3.835221"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PKW73_14870"
     CDS             complement(<1..1315)
                     /locus_tag="PKW73_14870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNA74627.1"
                     /translation="MEVIASVDGNGKVLSISQNSFEAWKLSADELVGRSIFDILYPED
                     MMAAQQALLSATFMKQPTQFECRVVRRDAIVVPMYWTAQWSDMDDAMLMVAQDHIKPK
                     DGEDPFKEARETMRFLMESLPLGLFLATVDGKIDFANKNLETLMETGALGLSERLIRS
                     VFPMDVRVGASGDSAYGPYIDRKTQMRVLTAKQRELPIEFSLRRVNVGGLPMYLGTVL
                     NTSERFELQRMRQKFIDSVQVQIKAPLAAIMMHIELTLEGVHGNLNDQGKRRAETILR
                     DIQEMMNAIEGILEVNKLQSGTFELDLTPNNIMVLLRQALMAMLSEIREKELVLEING
                     PELAVMVDEEKIMTVVMTLFANAVRYSPHGSKVRVQIIDAEEKVRVEIIDRGPGMTDD
                     QKDALFDPLRQPTVTEGGGGGNLGTILAAKQIVEKHGGTMGVISHQ"
     gene            complement(1452..>1821)
                     /locus_tag="PKW73_14875"
     CDS             complement(1452..>1821)
                     /locus_tag="PKW73_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74628.1"
                     /translation="VRQAMERARVKNPEIFEKAAGYSSEEGHRNRDKAVEKVGLILSE
                     WQAQYGDIPFSEREPSQSEPGPGRLPFWNTPLRYPLRNQQGEPIASSLTDLEQRQLAF
                     ACRIREIAVELLRAEQWFFC"
BASE COUNT          365 a          490 c          487 g          479 t
ORIGIN      
        1 cctggtggct aattacgccc atggtgccgc cgtgtttttc tacgatttgc ttggcggcaa
       61 gaattgtgcc gagatttccg ccgccaccac cctcggtgac cgtcggctgt ctgagcggat
      121 caaagagagc atctttttga tcgtctgtca taccgggacc gcggtcgata atttcgactc
      181 tgaccttctc ttcggcatcg attatctgca cacgcacttt tgaaccatgg ggggaatagc
      241 gcacggcatt ggcaaaaagg gtcatgacca cggtcatgat tttctcttcg tccaccataa
      301 ctgccagttc cggtccgttt atttccagca ccagttcttt ctctcttatc tcggaaagca
      361 tggccataag ggcttgccgt aagagaacca taatgttgtt gggcgtcaaa tccagttcga
      421 aggtgccgga ctgtagtttg tttacttcca gaatgccttc gatggcattc atcatttcct
      481 ggatgtctcg cagaatggtt tctgcccgtc ttttaccctg atcgttgagg ttgccgtgca
      541 ctccctccag ggtcagctca atgtgcatca taatggcggc caggggggct ttgatttgca
      601 cttgcacgct atcgatgaat ttctggcgca tgcgctgcaa ttcgaaacgc tcgcttgtat
      661 tcaagacggt gcccaggtac atgggcaacc cgcccacatt gactctgcgc agggaaaact
      721 cgataggcaa ttccctttgt ttagcggtta gcacgcgcat ctgggtcttt ctatcgatgt
      781 agggaccgta ggcactgtcg ccggaggctc ccactcttac atccatcggg aagacggagc
      841 ggatcagtcg ctcgctcagt ccgagggcgc cggtttccat gagggtttcc aaatttttgt
      901 tggcaaagtc gattttgccg tccacggtgg ctagaaacaa cccaaggggc aagctttcca
      961 tgagaaaacg catggtttcg cgcgcttctt tgaaagggtc ttcgccgtct tttggcttta
     1021 tatggtcctg ggccaccatg agcatggcat cgtccatgtc ggaccactga gccgtccagt
     1081 acatgggtac tactattgcg tcgcgcctga ctacccggca ttcgaactga gtcggttgct
     1141 tcatgaaggt ggctgacaac agtgcttgtt gtgcggccat catgtcttcc ggatagagaa
     1201 tgtcgaagat ggaccgaccc accagttcat cggcagacag tttccaggcc tcgaacgagt
     1261 tttggctgat gctcagtact ttgccgttgc cgtcgaccga tgcgattact tccacgggga
     1321 catccaggac actttattac aggtaaatga ctcttgggaa atgctaccac ccattcaaat
     1381 gggtggtcaa tattgccaac ctggcgcttt tcctgaaaaa cggcgattct tcagggctca
     1441 gtctagcggc ttcagcaaaa gaaccattgc tcggctcgca gaagttctac tgcaatttct
     1501 ctgatgcggc aggcaaaggc tagttgacgc tgttccaggt cggtgagact ggaggcgatg
     1561 ggctcgcctt gctggttgcg aagtggatag cgcagggggg tgttccagaa gggtaagcgc
     1621 ccgggaccag gctccgattg actgggttca cgctcgctga aggggatatc gccgtactgg
     1681 gcttgccact cactgagaat caagccaact ttctccaccg ccttatcgcg gttgcgatgc
     1741 ccctcctcgg aactgtagcc ggctgccttc tcaaatattt cgggattctt tacccgggct
     1801 ctctccatgg cctgtctgac c
//
LOCUS       DAOQNZ010000921         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_45929_length_1821_cov_3.613817, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000921 DAOQNZ010000000
VERSION     DAOQNZ010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45929_length_1821_cov_3.613817"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKW73_14880"
     CDS             <1..390
                     /locus_tag="PKW73_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA74629.1"
                     /translation="AFSPGAYLARLTRASVLEIVEKDWVRTARSKGLSYQATVLRHIL
                     RNSLIPVVTVLGPLTAIVITGSFVVEYIYAIPGMGRFFITAVMNRDYDLILGTTLVFA
                     LLLVIANTFVDVAYQLLDPRMRMNAED"
     gene            395..1270
                     /locus_tag="PKW73_14885"
     CDS             395..1270
                     /locus_tag="PKW73_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA74630.1"
                     /translation="MSEKRNSALQRLSKIPTAMVGLFIILSICVVALLSALGIELTPF
                     AYDQTTTSYLAGPSFEHPMGTDELGRDLLARVIYGARLSISIGLTTALTAFLIGSVYG
                     AIAGFAGSKIDGLMMRGVDIAYSLPDLLVMILVGVLLGRGTTGILIALGLVSWMGTAR
                     LVRAQFLQLKNEEFIEAAYAQGQSSLNVILKHLLPNALGPIIVALTFTVPSAILSEST
                     LSFIGLGLSPPACSWGTLASDGWRSLRAHPHLIFFPSLFIFLTVLSFNFLGDGLRDAL
                     DPRTRLDKFKGGSGH"
     gene            1356..>1821
                     /locus_tag="PKW73_14890"
     CDS             1356..>1821
                     /locus_tag="PKW73_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74631.1"
                     /translation="MNKLPGSFKVVGSALALLLALAGPFLQPACADERGWVLTQRSTN
                     FGDAYIYISPSGMKMVSPKLGCNIVTCAPGWSVAFYNDKTRQFYSTTYEKWTNDLTAK
                     MASKGQSLSDLPWTRGSVGGVSGLKATQYSIRNARLSPRGGARRQQVSAADCW"
BASE COUNT          334 a          513 c          495 g          479 t
ORIGIN      
        1 gccttctctc ccggggctta tctggccagg cttacccggg caagcgtgct ggagattgtg
       61 gaaaaggact gggtgcgcac ggcgcgctcc aaaggtcttt cctatcaggc tacggttctg
      121 cgccatattt tgcgcaattc tctcatccct gtggtgactg tgcttggccc cttgacggcc
      181 atcgtcatca ccggctcctt tgtggtggag tacatctatg ccatccccgg catggggcgc
      241 ttttttatta cggccgtgat gaaccgtgat tatgatttga ttcttggcac taccctggtt
      301 ttcgccttgc tcctcgtcat tgccaatacc ttcgtagatg tggcctacca gttgctcgac
      361 ccgcgcatgc gcatgaatgc ggaggattga gaacttgtcc gagaagcgaa acagcgccct
      421 gcaaaggttg agcaaaatac caacggctat ggtcgggctc tttatcattc tttccatctg
      481 cgtggtggca ttgctttccg ctctgggaat cgagctgact ccctttgcct acgatcagac
      541 caccaccagc tacctggccg gaccctcctt tgaacaccca atgggcaccg atgaactggg
      601 gcgagacctt ctggcgcggg taatttatgg tgccaggctt tccatctcca ttggtctgac
      661 cacggccctg acagccttcc tgattggtag tgtttacggg gccattgccg gttttgccgg
      721 cagcaagata gacggtttga tgatgcgcgg tgtggatatc gcctacagcc tgcccgatct
      781 gctggttatg attctggtgg gcgtgctttt ggggcgcggt actacgggta ttctcatcgc
      841 tcttggactg gtcagctgga tgggcacggc ccgcctggta cgggcccaat ttctgcaact
      901 gaagaacgag gaattcattg aagcggctta tgcccagggg cagagcagtc tcaatgtcat
      961 actcaagcac cttttgccca atgccctcgg tcccatcatt gtggccctga ccttcacggt
     1021 gccctcagcc atactctccg agtccacctt gagttttatc ggtctcggtc tttcgccgcc
     1081 ggcctgctcc tggggtaccc tcgcttccga tggttggcgc agtttgcggg cccatccgca
     1141 cttgattttc ttcccttccc ttttcatctt cctgacggta ctctctttca actttctggg
     1201 cgacggtttg agggatgccc ttgacccacg cacccgtctg gataaattca agggtggctc
     1261 ggggcactga tgagcatgaa ttcggcaaat ctgtttttat tagattggta gagggtagaa
     1321 aaccagtggt acgtgctttt aagtagagga caggaatgaa caagcttcca ggctctttca
     1381 aagtggtagg cagtgctctg gcgctgctct tggccctggc cggtccgttt ttgcagccgg
     1441 cttgcgccga tgaacgagga tgggtcttga cccagcgctc taccaatttc ggagatgcct
     1501 atatttacat cagcccctcc ggcatgaaga tggtctctcc caagctgggc tgcaatatag
     1561 ttacttgtgc tcccggctgg agtgtcgctt tctacaacga taagacaaga cagttttatt
     1621 ccaccaccta tgagaagtgg accaatgatt tgaccgccaa gatggcctca aaagggcaga
     1681 gtctctccga tttaccctgg acccgcggtt ccgtcggcgg tgtgtccggt ctcaaggcta
     1741 ctcagtattc catccgaaac gccagactgt cgccccgtgg tggcgccaga aggcagcaag
     1801 tcagcgccgc cgattgctgg g
//
LOCUS       DAOQNZ010000922         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46055_length_1819_cov_3.279478, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000922 DAOQNZ010000000
VERSION     DAOQNZ010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46055_length_1819_cov_3.279478"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKW73_14895"
     CDS             <1..347
                     /locus_tag="PKW73_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74632.1"
                     /translation="DGVSTIDEYWLRCKKFDKVIPLYKQESEAALKMFGASPRAALGT
                     CYLRLADAVIKQQSDSSHLVDKAALASACANLRKAIDIYKRSGNVDSNSVSDAEGYLR
                     DLQLKYGLPEGK"
     gene            complement(406..1566)
                     /locus_tag="PKW73_14900"
     CDS             complement(406..1566)
                     /locus_tag="PKW73_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74633.1"
                     /translation="MSAALAISVASSALFVLSTQLPGEADRPPLNQDQIARLQPRVRV
                     AFADWWLYQLFPFDRSDEKNTTFDAIAWVDEPAKAAFKKFSRYHDLLKTHEILFLKDH
                     TWEPQNLPNGSTRVTYAGYLVSLTDPSMNSHYVRFDLDIRSAPTGLRVCGWKMTYDPD
                     FQTTLSFLSKCKKHASSMQANRKAYDSIREAHLRQEEGKQQLALSLANEAISLNPDCA
                     LAYNFRADIRKDLHNYNQAIKDYDQAIVLKPDFALAFFCRAMLKDKLGDSMGAISDYG
                     ETLRLCPNFNYARINRGGILDMEGDRTGALADYDKLADIPEFREVIMAQRAQVKLEMG
                     NLIGAIIDCNESLKLNPKNTNAYRTRGIVWHTLGLQRFAESDFAKARGEGIR"
     gene            complement(1626..>1819)
                     /locus_tag="PKW73_14905"
     CDS             complement(1626..>1819)
                     /locus_tag="PKW73_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74634.1"
                     /translation="TCAEGIASMEGGATGGTTFDKNFAGTMAVIKARVKAFAANPQIA
                     PTPAGGNKPGASNNGSKDG"
BASE COUNT          382 a          418 c          512 g          507 t
ORIGIN      
        1 acgacggtgt aagcacaatc gacgaatact ggttgcgctg caaaaagttc gacaaggtaa
       61 ttcctctgta taaacaagag tctgaagcgg cgttgaagat gtttggggct agtccaagag
      121 ctgctcttgg tacctgctac ttgcggttgg ctgatgccgt aatcaaacaa cagagtgaca
      181 gttctcatct ggtcgataag gctgctcttg cctcggcttg tgcaaacttg cgtaaggcca
      241 tagacatata taagcgcagc ggcaatgtag atagtaattc ggtgagcgat gcggaaggtt
      301 atctgcgtga tctgcagttg aagtatgggt tgcctgaagg gaagtaaagg gtgacgagat
      361 ttgctggttt ggtgccaatc agtaaacttt cgtcaggcgt ctttgttacc gtattccctc
      421 cccccgcgct ttagcaaagt ctgattcagc gaacctttgc aaacccaggg tatgccagac
      481 tatgcccctg gtgcgatagg cgttggtgtt tttgggattg agttttagac tttcattgca
      541 gtcgataatg gctccaatca agttgcccat ttccaatttc acttgggccc gctgggccat
      601 gattacttcc ctgaattctg gaatatctgc tagtttgtca tagtctgcaa gagcgcctgt
      661 tcggtctcct tccatgtcga gtatgccgcc gcggtttatg cgggcgtaat tgaaattcgg
      721 gcagagcctc agtgtttcac cgtagtcgct gatagcaccc atgctgtctc ccagtttgtc
      781 ttttagcatt gctcggcaga aaaatgccag ggcaaagtcg ggcttgagga cgattgcctg
      841 gtcgtagtcc ttaatggctt gattgtagtt gtgcaagtct ttccgaatgt cggctctgaa
      901 gttgtaagcc agggcgcagt ccggatttag cgagatggcc tcatttgcca gcgaaagggc
      961 taactgttgt ttcccttctt cctggcgcaa gtgcgcttcc ctgatgctgt cataagcctt
     1021 gcgatttgct tgcatgcttg aagcgtgctt tttgcatttc gagagaaagg agagtgtagt
     1081 ctggaaatca gggtcatagg tcattttcca gccgcaaacc ctcaatcctg tgggagctga
     1141 gcgaatgtcc aggtcaaatc tcacatagtg ggagttcatg gacggatcgg tcaggctcac
     1201 caggtagcct gcgtacgtta ctctggtgct gccgttcgga agattctgcg gttcccatgt
     1261 gtggtcttta aggaagagaa tttcatgggt tttgagcagg tcatggtaac gagaaaactt
     1321 cttaaatgcc gcttttgcag gttcgtccac ccaggcgatt gcatcgaaag tggtgttttt
     1381 ttcgtcgctt cgatcgaagg gaaagagctg gtaaagccac cagtcggcaa aagctactcg
     1441 tacccgcggt tgcaggcgcg cgatttggtc ctggttcagg ggcggccggt ctgcttcccc
     1501 tggtagttgg gtcgaaagaa caaataaagc agaggaagct acgctgatgg ccagcgcagc
     1561 gctcattagt ctgaacttgg tgtatgagac aattatcggc atataaccat catacccttt
     1621 gtgttctatc catccttgct gccgttattg gacgcgccgg gtttgttgcc gccggcgggg
     1681 gttggtgcaa tctgcggatt ggcggcaaag gccttcactc ttgctttgat tacggccatc
     1741 gtgccggcga agtttttgtc gaaggtggtg ccgcctgtgg ctccgccttc cattgaagca
     1801 atgccttcgg cgcaggttt
//
LOCUS       DAOQNZ010000923         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46062_length_1819_cov_3.054422, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000923 DAOQNZ010000000
VERSION     DAOQNZ010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46062_length_1819_cov_3.054422"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PKW73_14910"
     CDS             complement(<1..417)
                     /locus_tag="PKW73_14910"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA74635.1"
                     /translation="MTSTIPPSQSQADDGFAVTVKTRSPGRPKDFALGMKRREDILEA
                     ATRLFAHRGFRNTDLKMVADELGVAKGTIYNYFESKNDLFVACVTFGMARLRLFVRAR
                     TRDISEPLELISCAVESYFHFFDEHPEVVELLVQERA"
     gene            complement(493..1590)
                     /locus_tag="PKW73_14915"
     CDS             complement(493..1590)
                     /locus_tag="PKW73_14915"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA74636.1"
                     /translation="MYLLRFVTIFALALLLILPGFSAVSFARAEKPKDEHIYIVSDRA
                     TLKLDGTGTKTGNMQDLRVREGVSEDALESDQESKTLTPYGQAAEGSADSASLSGRPF
                     SNSDFMEGKLKSAFFSDNGHDKLTWLATRDDESFKTTDLDSFLKACRGKKVVLFVHGC
                     CVSFKEMERQARDLDLGLKGAMGDDVVLLAYDWATPAWFYPGSLKNMETTQVRFNHFM
                     EALTSKLEPGSLCVVMHSLGANLMVRYLTGPQFGAMQKTNPMGQGQKTFHTLIFSRPD
                     MSLESFRPHLAEVAATAENSIILAAENDININLSGFVRSLGGKTFGSFRLGQMHNARE
                     LFPQMSVMDVGSLKLGHKIPYQLIGNLLGNL"
BASE COUNT          472 a          507 c          438 g          402 t
ORIGIN      
        1 agcccgctcc tgcaccaaga gttcgaccac ttccggatgc tcgtcgaaga agtgaaaata
       61 agactcaacg gcacaactga ttagttctag aggctctgaa atatcgcggg tacgcgcccg
      121 cacaaagagg cgcaagcgcg ccataccgaa agtgacacag gccacgaaaa ggtcattttt
      181 actctcaaaa taattgtaga tagttccctt cgccacaccc agctcatcgg caaccatctt
      241 caaatcagta ttgcgaaaac cccggtgggc aaacaacctg gtggccgctt ccaaaatatc
      301 ttcccggcgc ttcatgccca gagcaaaatc tttgggtcgc cccggcgaac gggtcttcac
      361 cgtaacggca aatccgtcat cggcctgaga ctgggaaggg gggatggtac tggtcatggc
      421 gttctagcgg ttgctcccgg agcaaacttt aattaaatga cccatcagtc aactaattat
      481 agcagccgct ccctaaagat tgccaagaag atttccgata agttgataag gaatcttatg
      541 accaagtttc aaggagccaa catccatgac actcatctga ggaaagagtt cccgagcatt
      601 atgcatctgc cccagacgaa aagagccaaa ggtctttccc cccaggctgc gcacaaagcc
      661 ggaaagattg atatttatgt cattttcagc cgccagaata attgaattct ccgccgtggc
      721 cgccacttcc gctagatggg ggcggaagct ttccagggac atatcgggac gactaaaaat
      781 aagcgtatgg aaggtcttct gcccctgtcc cataggattc gtcttctgca tggctccaaa
      841 ttgcggaccg gtcaaatagc gcaccatgag attggcgccc agactgtgca tcaccacaca
      901 aagactgccg ggctcaagct tggaggtgag tgcttccatg aagtggttga aacgaacctg
      961 agtggtctcc atattcttga gagagccagg ataaaaccag gccggagtag cccagtcata
     1021 agccagaagg accacatcgt cacccatagc ccccttcaaa ccgaggtcga ggtcccgggc
     1081 ctgccgctcc atttctttga agctgacgca acaaccgtgc acgaaaagca caactttctt
     1141 accccgacag gctttcaaga aggaatccag gtcagtggtc ttaaaactct catcatccct
     1201 ggtagcaagc caggtcagct tgtcgtgccc gttatcggag aagaaagccg acttcaattt
     1261 accttccata aaatcgctat tactgaaagg ccgccccgac aggctggcgc tatcggcgct
     1321 gccttccgca gcctgaccgt agggagtgag ggtcttactt tcctggtcgg attccagcgc
     1381 atcctcgcta acgccttccc gtacccgtaa atcttgcatg ttgccggtct tagtgcctgt
     1441 cccgtcaagc ttcaaggtgg cccggtcgct aacaatatag atgtgttcgt ccttaggctt
     1501 ttccgcgcga gcgaaggaca ccgccgaaaa accaggcaaa attagcagta aagctagagc
     1561 aaaaatggta acaaagcgca gcaaatacac ggaacaactc aaaggaacgg tcgcataatt
     1621 tcattctaca cttttgctca caatttcaag cacctctcaa cgccccctta ttcaccttta
     1681 ctgccgttct tgctcacttt gccgccagaa tgagttaccg gagacttgtg agcgggtgcc
     1741 ggggcagtct tatggctacc tttgctgcct gcaccaccac ggctatggtg tttttgagta
     1801 tgggccggcg aaggtgagg
//
LOCUS       DAOQNZ010000924         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46097_length_1818_cov_4.143505, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000924 DAOQNZ010000000
VERSION     DAOQNZ010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46097_length_1818_cov_4.143505"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1185)
                     /locus_tag="PKW73_14920"
     CDS             complement(121..1185)
                     /locus_tag="PKW73_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74637.1"
                     /translation="MNKLKSCLRKAAPTLLVAGLAVIAALTSHTLAVQAQSVPSDLSA
                     PGANQEATGKLRLRREPFQLSVESRGDGPVSGRKRASREGLNGLVNGSGLGSEAKNAS
                     QSPLRGQAQSNDPTAAAGLFSADQKAAFDTLPSARLKGKAELELLSKFDVEVIIDSSN
                     SMGKRDCPGGLSRWQWCGQQARGLADGLAAYVPGGLTLTTFASGYDVWEGATASDVAR
                     VFQTGGLSRGTRLSLPLKDRLDKHFGGKSGGRKPVLLAVITDGVPVPMTEPRLVADIL
                     VDASRKMKGPGDVTVVFLQVGANSFKGRRFLGLLDHQLVSYGAKYDCVTTVEFEELQR
                     VGLSQSLVAALKRAPSRMRD"
     gene            complement(1302..1694)
                     /locus_tag="PKW73_14925"
     CDS             complement(1302..1694)
                     /locus_tag="PKW73_14925"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNA74638.1"
                     /translation="MAPEKIEYEKLIEMAVAAANRAYAPYSKYKVGAALLASDGRIFT
                     GCNVENKNIGSGICAERTAIVKAVSEGANTFIACAVACATDYRCYPCGLCRQFLSEFG
                     PDLQIVVREENGYSIKTIGELLPAPSLK"
BASE COUNT          430 a          552 c          463 g          373 t
ORIGIN      
        1 cctgagaaga cctgacggca cgattgtcga gaacagcgaa agctcaaggc ggctcattgc
       61 cgagtttgag agcgctgcca ggagaccctg accctgagcc aaacggcacc taccgctcaa
      121 ttaatcacgc atccttgaag gagccctctt caaggccgct acaaggctct ggctcaggcc
      181 cacacgctgc agctcttcaa actcaacggt ggtgacacag tcgtacttgg caccgtagct
      241 caccagttga tgatctaaga gccccagaaa gcgccgccct ttgaagctgt tggcgcctac
      301 ctgcaagaaa accacagtta catccccagg ccctttcatc ttgcgcgaag catccacaag
      361 aatatcggcc accagcctcg gctcggtcat gggcaccggc acaccgtcgg tgattacggc
      421 aagcaaaacc ggcttgcgcc cgccgctttt gccgccgaag tgcttgtcca acctgtcttt
      481 aagtggtagc gacaatcttg tgccgcgact caagccgccg gtctgaaaga ctctggctac
      541 gtctgaggca gtcgctccct cccaaacgtc ataaccggag gcaaaggtag tgagagtcaa
      601 accgcccgga acataggcgg ctaagccgtc agccagaccc cgcgcctgct gtccgcacca
      661 ctgccagcgg gacaaaccgc ccgggcaatc tctcttgccc atggaattgg aactatcgat
      721 gatcacctct acatcaaact tcgaaagcag ctcaagctca gcctttccct tcaggcgagc
      781 ggacggcaaa gtatcaaaag cagccttctg gtcggcgctg aacaacccgg cagcagcagt
      841 cgggtcattg ctttgggcct gcccgcgcag cggcgactgg gacgcatttt ttgcctcaga
      901 gcccaagccg cttccattaa caaggccgtt caacccctct cgggaagccc gcttgcggcc
      961 ggagacggga ccatcacccc gactttctac cgaaagctga aagggctccc ggcgcagtct
     1021 cagcttgccg gttgcctcct gattagcgcc tggagcggac agatcggagg gaaccgattg
     1081 agcctgtacc gccaatgtgt gactggtcag agcggcaata accgccagac cagccacaag
     1141 taaggtcggc gccgcctttc taaggcacga ttttagcttg ttcaactgca cctgccttac
     1201 gattcggaac ctacaggatc taatacgtaa gccagggtca aaaagttcaa tgggcggatg
     1261 ttattttatc taccgaaaaa tcactgccgg actgtcccga cctacttcaa actcggcgca
     1321 ggcaaaagct caccgatagt tttgatggaa tagccgtttt cttctctcac gacaatttgc
     1381 agatcaggtc cgaactcact gaggaactgc cgacacagcc cgcaaggata acagcggtag
     1441 tcggtggcac aggccacggc gcaagctatg aaagtattag caccttccga aacggctttg
     1501 acaatggctg tgcgctcggc acaaataccc gagccgatgt tcttgttctc gacattgcag
     1561 ccggtgaaga tacgcccgtc tgaagccaac agagccgcac cgactttgta ttttgaataa
     1621 ggagcataag ctcgattagc cgcagccaca gccatttcta tgagcttttc atattcaatt
     1681 ttctctggtg ccaaaataaa cctctacttt gctaaaacgg ctgctgggta ccggcaagca
     1741 tggctcgagc cgcatccgga ctgatttcag gctttttgcc tgctgggctg tgttttggca
     1801 ccacgctgcc ggaatagg
//
LOCUS       DAOQNZ010000925         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46181_length_1817_cov_2.152667, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000925 DAOQNZ010000000
VERSION     DAOQNZ010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46181_length_1817_cov_2.152667"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1817
                     /locus_tag="PKW73_14930"
     CDS             <1..>1817
                     /locus_tag="PKW73_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74639.1"
                     /translation="AFTKLATGEFALTAKRLPTFNMAAGLNPETDGLSALPETPLVKM
                     FAESDGPGAVAYAIYNLNPGDFWGRMARKPLRLFQFPWNDCRLDFLGIPLLIQVFAHQ
                     ILTIFGFFGLVAFVSLPLALKRRQEDKTATAANKQPHPELNPANIDATRDVEIDLPAA
                     EPQMVSILVIGLVALSFICGHVAYLPFVADSRYGFTALPSLLLFATWCLSGQLKQRIK
                     KLAMIRLCIASAFIMLAFTLKEDAWRSLFATSNEAMTASILSLGALLLFIGCMLAVRT
                     LLGAGKTNTTAKVLALLSSYLLLTLMLAASMMGKEYPIDWEARLSDKEEFVRIVDLPD
                     SIDLSDTAFVLVNLKGDWRGVRLKVNEKSIDTQPISLLQLNETADLSNCYRTFGYILR
                     TGTDGINQWRAFRFDSKLLRPGAQNTIALHSEKEETGRASLTGSTKYNAAPSLEIFSP
                     TNLCRSPFSIEPRLKVKLPQSVTHAISVRKTGGSVSKDLSASIGTQNGQYHMFLLAGR
                     ARETAPAQDDRNLAAAQPSTYIEVKQSPQPRKQTQTSRCAYLGEARLPEEVLAGRHLL
                     LRLTGEVDCASPIDARIVLNDLRLMQAVEDLTSEPARIP"
BASE COUNT          437 a          479 c          452 g          449 t
ORIGIN      
        1 ggcttttaca aagctggcaa ccggcgagtt tgcattgacg gcaaagcgac tgccgacctt
       61 caatatggcc gccggactga atccagaaac tgacggcttg agtgcattgc ctgaaacacc
      121 actggtgaag atgtttgcag agagcgacgg tccaggcgcg gttgcctacg ccatctacaa
      181 cctgaatccg ggggacttct ggggtcggat ggctagaaaa ccattgcggc tttttcagtt
      241 tccatggaat gactgccgtc tcgattttct tggaattccg cttttaatcc aggtttttgc
      301 gcaccaaatt ctgactatat ttggattttt cggcttggtc gcttttgttt ctctgccgct
      361 ggcactgaaa cgccggcaag aggacaagac tgccactgcc gcaaacaagc aaccacaccc
      421 ggaattaaac cccgcgaaca ttgatgcaac acgggatgtc gaaatagatt tgcctgccgc
      481 cgaaccacaa atggtttcaa tacttgttat aggactggtg gcattatctt tcatttgtgg
      541 gcacgtcgcc tatctgcctt tcgttgccga cagtcgctat ggctttaccg ccttgccttc
      601 attacttctc tttgcgacct ggtgcctttc gggacagctg aagcagcgca tcaagaaatt
      661 agccatgatc aggctatgca tcgctagcgc cttcataatg ctcgcattta ctctaaaaga
      721 agatgcgtgg cgcagtcttt ttgcgaccag caacgaggcg atgaccgcat caattctctc
      781 actgggagcc ttgttgcttt tcattggctg catgcttgct gtgcgcacat tgctgggtgc
      841 gggaaaaaca aataccacag ctaaggtgct ggcgctgcta tcttcatacc tgcttctgac
      901 tctgatgctg gcagcctcga tgatgggcaa agaataccct atcgactggg aagcacggct
      961 gagtgataaa gaagagttcg ttcgcattgt cgatctgcct gattctattg acctgtctga
     1021 tacagctttt gttcttgtaa atctaaaggg agactggcgc ggggtcagac tgaaggtcaa
     1081 tgagaagtcg atcgacactc aacctatctc tctgcttcag ctgaacgaaa cagccgacct
     1141 cagtaattgc tatcgaactt ttggttatat tctgcgcaca ggaaccgatg gaatcaatca
     1201 gtggcgtgcc tttcgattcg attcgaaact gctgcggccg ggcgcacaaa atacaattgc
     1261 actccactct gaaaaggagg agaccgggcg ggcgagcctg accggcagta ctaaatacaa
     1321 cgcggctcca tcattggaga ttttctcgcc gaccaattta tgcagatctc ctttttcaat
     1381 cgagcctcga ctgaaagtca aattaccgca atccgtgact catgcaatct cggttcgaaa
     1441 aactggtggc tccgtaagca aagacctttc tgcttcaatc gggacacaaa acgggcaata
     1501 ccatatgttt ttgcttgccg gtcgcgcgcg agagacggcc cctgcgcaag acgaccgtaa
     1561 ccttgccgcc gctcaaccct ctacatatat agaggtaaaa caatcgccgc aaccaagaaa
     1621 acaaacgcag acaagccgct gtgcatatct aggtgaagcg cgcttgccgg aagaagtact
     1681 ggccggtcgc catttgctcc tcaggctgac cggagaagtg gactgcgcca gcccgattga
     1741 tgccagaatt gttctcaatg acttgcgctt gatgcaggcg gtcgaagact tgacctcaga
     1801 gcctgctcgc atcccgg
//
LOCUS       DAOQNZ010000926         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46275_length_1815_cov_2.674432, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000926 DAOQNZ010000000
VERSION     DAOQNZ010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46275_length_1815_cov_2.674432"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKW73_14935"
     CDS             <1..247
                     /locus_tag="PKW73_14935"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNA74640.1"
                     /translation="SPVLVKILQAMPGRLPYLGVIGSKAKAARLKADLLAAGVEPRHA
                     EAFHCPIGLPFGTNAPGEIAVSVAAQLLSVRDAALNS"
     gene            complement(197..1057)
                     /locus_tag="PKW73_14940"
     CDS             complement(197..1057)
                     /locus_tag="PKW73_14940"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNA74641.1"
                     /translation="MSKPKAWLFLDFDNTMMATEHLALPSLIARFNELYESQIGRPLT
                     EAEFHEHFHGQARQSLCLSMSKHFGINVEGELLYHQREWRMMQMLQQKGVDMAPALLE
                     TLTLLQKSGVKLAFVSNNSIQRALAAMRYSTNGRGEELARLFGTAYFEAGDVQKPAPD
                     IYLRALKQLQADPQESYAVEDSVTGVKAAVAAGITTFAYTGFAGTINGCTDELEKKLM
                     AAGARTCFAHWNDFPALLKKEQESATVSSDSKDSSDSKDSSVIASSTGLSAVSGIQGS
                     VPHAEQLSSD"
     gene            complement(1156..1707)
                     /locus_tag="PKW73_14945"
     CDS             complement(1156..1707)
                     /locus_tag="PKW73_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74642.1"
                     /translation="MPSDLPGNVPTPVIGGLAAAILVPIIMNKLASTNAPARLEGEHK
                     VVSYSKGYFASMYICTFFFSALSVLAYLNPGKTGPQAIVVPIMVFLFFAAMGLFTVVL
                     MNKTRVYFNSEQVRGADMLGRRHVYRWNELKGVEYVAWAQGLKLSGPDGGSIWVSPVM
                     SGFAEFQEELERQCGHLWQNTAQ"
BASE COUNT          394 a          482 c          472 g          467 t
ORIGIN      
        1 cagcccggtg ctggtcaaaa tcttgcaggc catgccgggc cgtcttccct acctcggtgt
       61 aatcggcagt aaggccaagg ctgctcgact taaggccgat ctgctggccg ccggcgtcga
      121 accgcgccac gccgaggctt ttcattgccc gatcgggctg cccttcggca ctaacgctcc
      181 tggtgaaatt gctgtctcag tcgctgctca actgctcagc gtgcgggacg ctgccctgaa
      241 ttcctgagac cgcactgagc cctgtggaac tggcgatgac cgacgaatct ttcgaatccg
      301 atgaatcttt cgaatccgat gaaacggtgg cgctttcctg ttctttcttc aataaagccg
      361 gaaaatcatt ccagtgggcg aaacaggttc tggcgccggc tgccataagc ttcttctcaa
      421 gctcatcggt gcagccgttg atggtgccgg caaagcctgt ataggcaaaa gtggttatac
      481 cggcagctac tgccgctttc acaccggtga cgctgtcttc aacagcatag ctttcttgcg
      541 gatctgcttg cagttgtttc agtgcgcgca ggtagatatc cggcgccggt ttctgcacat
      601 cacccgcttc aaaataggct gtaccgaaga gtcgtgctaa ctcctcgccg cgcccattgg
      661 tgctatagcg catggcggcc agggctcttt gtatggaatt gttggaaaca aaggctaact
      721 tgacgccgct tttttgtaag agtgtcagtg tttccagcaa ggctggtgcc atatctacac
      781 ccttttgctg caacatctgc atcatgcgcc attctctctg gtggtataga agttcaccct
      841 ctacattgat gccgaagtgt ttgctcatgc tcaaacagag tgattgtctg gcctgaccat
      901 gaaagtgttc atgaaactca gcttccgtca gcggtctgcc gatctggctt tcataaagct
      961 cgttgaatct ggcaatgaga gaaggcaacg ccaggtgctc ggtagccatc atagtattgt
     1021 cgaaatcaag aaatagccag gcttttggtt tactcataat aaggccgtag tttgtcggtt
     1081 gtctccagcg gcgagagcgc gctcagcaca ggttagctca agtgcgtaag ctttgcctta
     1141 accgtggctt aatggttatt gggcggtgtt ctgccagaga tgtccgcact gcctttctaa
     1201 ttcttcttga aattcggcaa agccgctcat tacgggagaa acccagattg agccgccatc
     1261 tggaccgctt aatttgagac cctgggccca ggccacatat tcaacgcctt tcagttcgtt
     1321 ccatcggtat acatgccgtc gtccgagcat atcggcgccg cgcacctgtt cactgttgaa
     1381 gtagacccga gttttattca tgagcacgac tgtgaaaagt cccatggcgg caaagaacag
     1441 gaagaccatg attgggacaa caatcgcctg gggtccggtt ttgcctgggt taagataggc
     1501 aagcaccgag agcgcgctga agaaaaaggt gcaaatatac atggacgcaa agtagccttt
     1561 actgtagctt acgaccttgt gctcgccctc aagtctggct ggtgcgtttg tgcttgccag
     1621 cttattcatg ataattggaa caagaatcgc cgctgccaga ccgccgataa cgggagtagg
     1681 gacatttccg ggtagatcgg acggcattgg ctcgttcctc ttttgacaat tctcgctatt
     1741 atttacccag tcttgctctt aaagtcttca ggacttcgcc ttcttcagga aagcgtccga
     1801 gcatcagctt cgaga
//
LOCUS       DAOQNZ010000927         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46315_length_1814_cov_3.450256, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000927 DAOQNZ010000000
VERSION     DAOQNZ010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46315_length_1814_cov_3.450256"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKW73_14950"
     CDS             complement(<1..374)
                     /locus_tag="PKW73_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74643.1"
                     /translation="MPLPQFKNSICFLLPILAVSTLSLPVQPAPAQTKPNQKPEQKTK
                     AAGATYRIPVKDKYGLPIAQIESLLKIRNSKGALAALTKVPRSTGDTDRRSMIAGAAK
                     ANDNDFDGALLLFSRIKVFEGTP"
     gene            621..1697
                     /locus_tag="PKW73_14955"
     CDS             621..1697
                     /locus_tag="PKW73_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74644.1"
                     /translation="MESVGNKNIKTEQSVRSFYMGFSPWPYDLTLEAVQWTDAAIKAH
                     GDIIEQHFEEGVPWPEALANKPYDGAMQQEMADRSRRMGDYKRVVSINPINMARNGLA
                     DYRGKSCNMPLSGQWKKRRLNDPAVKQAYLQYCNQVIQSLRPDFLLIGVEVNLLQRCK
                     DSSWQDYLELHRYIYGALKRSHPGLPVMASVVCTSYFPGLCSEDNAGDQRAKLKDLLP
                     YVDVLGFSVHPFMSSWTAEKVPDQSFFRELFALAAGKPFAITESSYPAQPWQMQVGLW
                     PVKFNGSQEKQAAFLKAMLDASLVCKPRFISWYSIRDYDQLCRKLPNIPLMIVWRDTG
                     LFDENGQPRQALKIWDAVFKRRRQ"
BASE COUNT          401 a          425 c          515 g          473 t
ORIGIN      
        1 ggtgtgccct caaaaacctt gattctggag aacagtagta gggctccgtc aaaatcgtta
       61 tcattggcct tggcggcacc cgcaatcatg cttcttctat ctgtgtctcc tgtcgagcga
      121 ggcaccttgg taagagcggc cagggctcct ttcgagttcc ttatcttgag cagggattca
      181 atttgagcta tgggcagacc atacttgtct ttgactggga tacgataggt ggctccggca
      241 gccttagttt tctgctccgg cttctgattt ggttttgttt gggccggcgc gggctggacc
      301 ggcagcgata gcgtcgacac cgccaggatg ggcaagagaa aacagatcga atttttgaat
      361 tgggggagag gcatttagat tttgggcgtg ctttactcca gtctatccgt ttccgtgagg
      421 ttttggtgtt gaagttctgt tctttctcga cggcgctatc atcaagattg ctatttgcta
      481 gagtaagcgt gtgtatcgct ttcggtttca ttcgacaagg caggtgcgtt atccctcagc
      541 tcttaaaagt tgccggcctg tttggcctac tcactctgtc ctggataatc ggaatggttg
      601 ggggtgattg ccgcgagggg atggagtctg tcggcaataa aaacatcaag acagagcagt
      661 ctgtacgttc gttttacatg ggtttcagtc cctggcctta cgaccttacc ctggaggcgg
      721 ttcagtggac tgacgcagcc atcaaagccc atggcgacat tatcgagcag cactttgaag
      781 aaggggtacc ctggccggaa gcgctcgcga acaaacccta cgacggggcc atgcagcagg
      841 agatggccga tcgttccagg cgtatgggcg actacaaaag agttgtttcc atcaatccca
      901 tcaatatggc tcgcaacggt ctggcggatt atcggggcaa gagctgcaat atgcccttga
      961 gcgggcagtg gaagaagcgg cgcctgaatg atccggccgt caagcaggct tatctacaat
     1021 actgcaatca agttattcaa agcctgcgcc ctgacttctt gctcatcgga gtggaggtga
     1081 atctgcttca gcgctgcaaa gatagctcct ggcaagacta cctggaattg caccgctata
     1141 tctatggtgc tctcaagagg agccatccgg gtcttccggt tatggcttcg gtagtgtgta
     1201 cgtcctactt cccggggctt tgttctgagg ataatgctgg agatcagcgc gccaagctga
     1261 aagacctgtt gccgtatgtt gatgttctgg gtttttccgt acatccgttt atgtcatctt
     1321 ggaccgctga aaaggtgccg gatcagtctt tctttcgaga actgttcgcc cttgccgcag
     1381 gcaaaccatt cgccatcact gagagcagtt acccggccca gccgtggcag atgcaggtag
     1441 gactctggcc ggttaagttt aacggcagcc aggaaaaaca agcagcgttc ctcaaggcca
     1501 tgctggatgc tagtttagtc tgcaagccgc gtttcataag ttggtatagc attagagatt
     1561 atgatcagct ctgccgcaag ttgcccaata ttcctttgat gatcgtctgg cgtgataccg
     1621 gtttgtttga tgagaacggg caacccaggc aggcattgaa aatatgggac gccgtattta
     1681 agcgccgcag gcagtaacag gtggactgac ataggtgcgg tgcggccgat gaaagagtaa
     1741 gaattctgta tggagactct tccggaagtg aagccagagc tgattttgca gagaactcat
     1801 gttggaaaag gatt
//
LOCUS       DAOQNZ010000928         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46363_length_1813_cov_3.864050, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000928 DAOQNZ010000000
VERSION     DAOQNZ010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46363_length_1813_cov_3.864050"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..858)
                     /locus_tag="PKW73_14960"
     CDS             complement(103..858)
                     /locus_tag="PKW73_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA74645.1"
                     /translation="MSLLVNGVSKRFYDKARGEFYGCKEISFAVNPGEVYGLLGPNGA
                     GKTTTLRVVSTIYSPTAGGASINGVDIVRSPQAARKMLGFLSSTSGLYERLTAREILT
                     YFGRLCDMPEDVLNKRIESLIDALEFRSYADTRVAKLSQGTRQKVSIARSIIHDPLLM
                     ILDEPSTGLDVLAAYAMHEFVRQERADGKCILLSTHTMSEAEKLCDRIGIISEGRLLA
                     SGTIDELKELTGKANLEDVFVHLVRNAAQAAVT"
     gene            complement(855..>1813)
                     /locus_tag="PKW73_14965"
     CDS             complement(855..>1813)
                     /locus_tag="PKW73_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNA74646.1"
                     /translation="LEGPHLTAYVGHSFDSYLKTEKLKDALDNFQRDELKRQFALKGL
                     KDTRGAYGAKMVNLHPLKQESYSLHFVMLLVSVMMMGVGVCYPAITATTEEFERKTIE
                     STMLLPVSRDAIIFAKLLSVCLFGVFTGAVNFFSILAVFALLIFTASQVRTIDINELK
                     SLLTPVQVLLVSSSYLIMGLLLASLMMVVTSFCRTVRSAQNWVSIPMLLVMVLPAISL
                     LPSLTLGKGLALVPFLGLALTIRQFFTAPTVSFEQYLALFSSLVYAVLLTGIAGRLMF
                     EFDGDFVQHLQAGISGRKGGVKPSSPPQSLEVHPDQEAAEKL"
BASE COUNT          491 a          478 c          478 g          366 t
ORIGIN      
        1 gaccaagcaa tacccccgga acaagagcct tatagtcgcg caacatgtcc ataagctcct
       61 tgccggtgac aaggagtatc tgggaaagga tgggattgcc gctcacgtta ccgccgcctg
      121 cgccgcattg cgcaccaggt gaacgaagac gtcctccaga ttagccttac cggtaagctc
      181 ctttaattca tctatggtac cggaagccaa aagacgcccc tcgctgatga tgccgatgcg
      241 atcgcaaagc ttttcagcct cactcatggt gtgggtggaa agcagaatac acttgccgtc
      301 tgcccgctcc tgtctgacaa actcatgcat ggcataggcc gccagaacat caagaccggt
      361 gctgggttca tcgagaatca taagcaaagg atcatgaatt atagagcgag caatggaaac
      421 tttctgcctg gttccctgtg aaagcttagc tactcttgtg tcggcatagc tgcgaaactc
      481 cagggcatca atcaagcttt caatacgctt attcaatacg tcctcaggca tgtcgcaaag
      541 ccgtccgaaa taggtaagaa tttctctggc ggtgaggcgc tcatagagtc cggaggtgga
      601 agagaggaag cccaacatct tgcgagcggc ttgcggactc ctgacgatat ccacaccatt
      661 tatgctggca ccaccggcgg tgggcgaata tatggtggaa acgacccgca aagtggtggt
      721 tttgccggct ccattgggtc caagcaagcc gtacacttcg ccgggattga ccgcaaagct
      781 tatttccttg cagccgtaga attcacctct ggccttgtca tagaagcgct tagatactcc
      841 atttacaagc aaactcataa cttttcggcg gcctcctgat cggggtgaac ttctaatgat
      901 tgcggcggag agctcggctt cacaccgccc tttcgcccgg aaattccggc ttgcagatgc
      961 tgaacaaaat cgccgtcgaa ttcgaacatg aggcgcccgg cgatgccggt caacaaaaca
     1021 gcatagacaa gggaggaaaa cagagccaga tactgttcaa aagaaacggt aggagccgtg
     1081 aagaactgcc tgatggtcag ggccagaccc agaaatggta ccagggccag ccccttaccc
     1141 agagtgaggg agggcaaaag cgatatggcc ggcagcacca tcaccaacag catgggaatg
     1201 gaaacccagt tttgagccga gcgcaccgtg cgacagaaac tggtgacgac catcatgagg
     1261 gaagccaata agagccccat tatcaggtag gaagaagaga ccaataagac ttgcaccggc
     1321 gtaagcaggc tcttcaattc attgatatcg atggtgcgaa cctggctggc ggtgaaaatg
     1381 agcagggcaa agacagccag aatagagaag aaattgacgg cccctgtgaa aacaccgaaa
     1441 aggcaaaccg ataacagttt ggcaaaaatt atggcgtccc gagaaaccgg caagagcatg
     1501 gtgctctcaa tggtcttgcg ctcaaactct tcggtggtag cggtgatggc ggggtagcag
     1561 acccccaccc ccatcatcat cacagaaaca agcaacatga caaagtgcag ggaataagac
     1621 tcctgcttga gcgggtgcag attgaccatt tttgcaccat aggcgccgcg cgtatccttc
     1681 aggcctttaa gggcaaactg cctcttcaac tcgtccctct ggaagttatc gagggcgtcc
     1741 ttcagttttt cagtcttcaa atagctgtcg aagctatgcc cgacataagc cgtcagatgc
     1801 ggaccctcca gag
//
LOCUS       DAOQNZ010000929         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46373_length_1813_cov_3.317406, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000929 DAOQNZ010000000
VERSION     DAOQNZ010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46373_length_1813_cov_3.317406"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1813)
                     /locus_tag="PKW73_14970"
     CDS             complement(<1..>1813)
                     /locus_tag="PKW73_14970"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA74647.1"
                     /translation="AHSHGVVHRDIKPSNIMLALREDGTVQIKLLDFGIAKMRDLDDH
                     TIQDLTKTGEALGTPLYMSPEQIKGTRVDSRADIYSFGCVMYMCLTGVPPFVGENKLH
                     TMDKHVSEIPLTLKEASIGLDFPPELESCVMRCLAKEPEQRYQSSDALRRDLVSIACQ
                     LGMVKMPPGMVSPTESFLDDVASNPPLSEELAQAVTEFTSLELSTLCLTDPPAAVERK
                     AGGSSDLTVDAKSMGLTGSATRSQSQRNTVADLNRTGQRQLPVPYGGAGSSPGARRHE
                     IELAEDDAQEISYVDLEPSPWQRGRVRLFAALALLMVLGGSVSWYCLLGPGVHQAEGD
                     KVKTAADAPPVSQPLPTAAVKPAEVKLTEKDPLYEIEEENGDESLVKMIAARPFMTRL
                     EPRNISVTDAAMPHVARLKFLEVFSLWDSKVTAKGLSYINKAKLLAFGLYSCSVDDAI
                     VPAVLQFKNLTQVGFRDCKGITDSGGAKLAALKNLTTVDLAGTSVSDKTAAALAGMPR
                     LLTVLLDGTDLTDAGLKDLCRNGAIIWLSLDKCKVSETGLASLRKMPKLVKLSVSGCD
                     VGDGFVDTLLPLKHLEVVNLSNTRITDKGLLKLSGRKQ"
BASE COUNT          431 a          501 c          475 g          406 t
ORIGIN      
        1 ctgcttgcgc ccggaaagct tcaaaagccc cttgtctgtg attcttgtat tggagaggtt
       61 taccacttct agatgcttga ggggtaaaag ggtgtcgacg aagccgtccc cgacatcgca
      121 gccggaaaca ctcaatttaa cgagcttagg catttttcta agggacgcaa gacctgtttc
      181 actcacttta cacttatcga gagagagcca gatgattgct ccattccggc aaagatcttt
      241 caaaccggca tcggtcaaat cggtgccgtc cagtaaaaca gtcaaaagcc tgggcatgcc
      301 ggctagagca gccgcggtct tatctgaaac actggtaccg gcaaggtcta ccgtagttag
      361 atttttgaga gccgccagtt tggcgccgcc actgtcggta atgcctttac agtcgcgaaa
      421 gcccacctgg gtaagattct taaactgcaa tacggcggga acaatggcat catcaacgga
      481 gcagctatag agcccaaagg ccaaaagttt ggccttgttg atataagaca agccttttgc
      541 cgtcacctta gagtcccaga ggctaaagac ctcaaggaat ttgagcctgg caacgtgcgg
      601 catggccgcg tcggttacac tgatgtttcg aggctccaga cgggtcatga agggcctggc
      661 ggcaatcatc ttaactaaac tttcgtcgcc gttttcttct tcaatctcat agagtggatc
      721 tttctcagta agttttacct cagccggttt tactgctgct gtcggcagcg gctggctaac
      781 cggaggagca tccgctgcgg tctttacttt gtcgccctca gcctggtgca cacccggtcc
      841 gagcaaacag taccaggaca cagatccacc caggaccatc aaaagtgcca gagcggcaaa
      901 caatctgacg cggccgcgct gccaggggga cggttccagg tcaacataac ttatctcctg
      961 ggcatcatcc tcagccagct caatttcatg cctgcgggct cccgggctgg aaccagcgcc
     1021 gccatagggc acaggcaact gcctttgccc ggtacgattc agatcggcta cagtattcct
     1081 ctgactctga gaccttgtgg cagagccagt cagccccatt gacttagcat ccacagtcag
     1141 atcggaagag ccgcccgctt tccgctccac tgctgcgggc gggtcggtca aacagagggt
     1201 ggaaagctcc agagaggtaa actcggttac agcctgcgcc agttcctcgg aaagaggagg
     1261 atttgaggcc acatcatcga gaaaggactc ggtaggactc accatgcccg gtggcatctt
     1321 gaccataccc aactggcagg caatggacac cagatctcgg cgcaaggcat cgctcgactg
     1381 atagcgctgc tccggttctt tagccagaca gcgcattaca caggattcca gctcaggagg
     1441 aaaatccaga ccaatggatg cctccttcaa ggtaagcggt atttcgctca catgcttgtc
     1501 catggtgtgc agcttattct cgcccacaaa aggcggcaca ccggtcaaac acatgtacat
     1561 gacgcagccg aaagaataaa tatcggcgcg gctatctaca cgggtacctt tgatctgctc
     1621 cgggctcata tagagcgggg tgccgagcgc ttctccggtc ttagtcagat cttgaatggt
     1681 atggtcgtcg agatcgcgca tcttggcgat gccgaaatcg agcagcttaa tttgcacggt
     1741 gccgtcttct ctcaaagcaa gcatgatatt gcttggcttg atgtcacggt gcacaacacc
     1801 atgactatgg gcg
//
LOCUS       DAOQNZ010000930         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46458_length_1811_cov_4.102506, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000930 DAOQNZ010000000
VERSION     DAOQNZ010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46458_length_1811_cov_4.102506"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PKW73_14975"
     CDS             <1..979
                     /locus_tag="PKW73_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA74648.1"
                     /translation="EMKSGTITVNPDTPEAYTVQLTEGEVVQVGRKPATGGVKKLVLP
                     FPEVSGQHAEVRCKPDGWTVVDSGSTNGTTLNGARLTPGREYHVRSGDRIKIAQYELI
                     IFPPEIAGQADDESDDAQDRTQFRIQMMNATILVGDIKGFTTLMERHAGQPTLVMQAA
                     QKVFDNLNEEINRNFGQLEKIAGDAIMAYWQGQGADPKSAGSVSACQACYTALKLRGL
                     TAKLGADQTCWPFPYHPLMLDMALATGPVASGNLGHSVSNPALLGDTANLVFRLEKLI
                     GDDRPGDIIVEGATYELAKANFKFDFLGQTNVKGRTKPVDVYRLIEPIS"
     gene            982..>1811
                     /locus_tag="PKW73_14980"
     CDS             982..>1811
                     /locus_tag="PKW73_14980"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNA74649.1"
                     /translation="MRSIEQAVGRLLLGRIPGPTLNCEHRKQLAAGRLGGITLFKENC
                     RSLEQLARLAHDIVQSSMHVPVLTVDQEGGAVQRFEEALSPIPSPMALAALGEDELAR
                     VTEIGASQLQKLGFNLLLAPTLDLQTNPLNPIICTRAFGSSPQQVSNAGLKAIESIER
                     AGLRACGKHFPGHGSTSEDSHLALAVVAKSRAELLAEDLKPFIDNLPYLSSILVGHIW
                     LSQIDEEPIPATLSKKIVSGLLRQDLGYDGLLVSDDMLMKGLTSRYGLSEACILALEA
                     G"
BASE COUNT          426 a          514 c          494 g          377 t
ORIGIN      
        1 agaaatgaag tcgggaacta taacggtcaa ccccgacacg ccggaagcgt acaccgtaca
       61 gcttaccgaa ggcgaggtag ttcaggtcgg gcgcaagccg gccacaggcg gcgtcaaaaa
      121 attggtgcta ccctttcctg aagtctccgg tcagcacgca gaagtgcgtt gtaaacctga
      181 tggctggacg gttgtggaca gcggcagcac caatggtacg accctgaatg gcgctcgcct
      241 cacccccggc agggaatacc atgtgcgtag cggcgaccgc atcaaaatag cccaatacga
      301 actgatcatc ttccctccgg aaatcgccgg acaagcggat gatgaatcgg acgacgccca
      361 ggatcgcacc cagttccgta ttcaaatgat gaatgccacc attctggtgg gagacatcaa
      421 aggtttcact accctgatgg aacgccatgc cgggcagccc accctggtga tgcaggccgc
      481 gcaaaaggtc ttcgacaatt tgaacgagga gatcaatcgc aatttcggac aactggagaa
      541 aattgccggg gacgccataa tggcttactg gcagggtcag ggcgccgatc cgaaaagcgc
      601 cggttcggtc tcggcctgcc aggcctgtta cacagccctc aagttgcgcg gtcttaccgc
      661 caagctggga gcggatcaaa cttgctggcc tttcccctac cacccgctta tgctggacat
      721 ggctctggct accggcccgg tggcctcggg taaccttgga cattccgtct ccaatccggc
      781 ccttttggga gacacggcaa acctggtttt ccgcctggag aaactgattg gggacgatcg
      841 accgggagac ataatcgtgg aaggtgccac ttacgaactg gctaaagcca acttcaaatt
      901 tgattttctc ggacaaacca atgtcaaagg tcgcaccaag ccggtagatg tctatcgctt
      961 gatcgagccc atctcttagg tgtgagaagt atcgaacaag cagtcggtcg tcttctgctc
     1021 gggcgcattc ccgggccgac tctcaattgc gagcaccgca aacagcttgc cgccggacgt
     1081 ctgggcggta tcacgctctt caaggaaaat tgccgcagcc tggagcaact ggctcgtctg
     1141 gctcacgaca tcgtccagag ttccatgcac gtgccagtgc tgactgtgga tcaagaaggc
     1201 ggagcagtac aacgctttga agaggctttg agtcctatac cttcccccat ggctctggcc
     1261 gccctgggag aagatgaact ggccagggtt acggaaatag gagcgagcca gcttcagaag
     1321 ctgggtttta atttgctact ggcaccaacc ctcgacctgc agaccaatcc cctcaatcct
     1381 ataatttgca cccgcgcctt cggttcttcc cctcagcagg taagtaatgc cggtctgaaa
     1441 gccatagaga gcattgaaag agccggacta cgggcctgtg gcaagcactt tcccggacac
     1501 ggctccacca gcgaagattc tcacctggca ctggctgtgg tagcaaagtc ccgggcagaa
     1561 ttgctggccg aagatctgaa gccatttata gataacttac cttatctgag ttctattctg
     1621 gttggtcaca tctggctctc ccagattgat gaagagccga ttccagccac actttccaag
     1681 aaaatagtca gcggcctctt gcggcaggat ttaggctatg acggacttct ggtctccgac
     1741 gacatgctca tgaagggatt gaccagtcgc tacgggctgt cggaagcctg cattctagcc
     1801 ttggaagccg g
//
LOCUS       DAOQNZ010000931         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46573_length_1809_cov_3.535348, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000931 DAOQNZ010000000
VERSION     DAOQNZ010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46573_length_1809_cov_3.535348"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..>1809
                     /locus_tag="PKW73_14985"
     CDS             148..>1809
                     /locus_tag="PKW73_14985"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA74650.1"
                     /translation="MTSSGGESKSKIDTLTSLRFFAAAAIVVYHSRGHFGIPSDYLSP
                     FVLDQGVSFFFVLSGFILSLVYGEKLKEGGLNAGSRLADILSFIVARFARIWPLHVAT
                     LVLVAFLLPEKWNHDLSPVNLLASLCLMQSWIPINDVSFAFNGVSWSLSAELFFYLSF
                     PFLIVAYEKNWLRNSLLALGLMTSLSCLCAICGFPEHVGNGVSMHELMYVSPLSRVFE
                     FSLGIGAFALARRWHINWRAGESAAAASSFKLRLVFTIIELAVLLIGLFVAYKSTRIS
                     SFLAEAMDSLLPGVSAPLNTWLMHGGSVALVFALMIIVFSFGRGLLSKVLSWPLLVYL
                     GEISFSVYLSHRLLLHYYYNHFATVDGAMPIAVYWLVLLSVSHLLYVFVECPCRSLIV
                     GLFRRFQKGARPEGVSSSWSQILPQFRLSLLNRFFLAAEALFLLALPALLFSGPALAR
                     LEGSQLTPTVLDSVSKPLARLGDDVELLSVSGRDDIGGKRKVILTWRALRHQKLNGFV
                     TLHLYTPDNVYYKRRDYKRAWREEEVASGQIWRDEVLLNREETATT"
BASE COUNT          319 a          475 c          488 g          527 t
ORIGIN      
        1 tccggtcggg cgtctgataa acggaggcgt ttttcagggc ctaaagatat cggtattgat
       61 tagtagatgt gatttttacc taaggaaaat gccctgctcg ggactgtatc atgacaagtc
      121 ggcagccagc gtcacttgag gttccagttg accagttccg gcggggagtc caaaagcaag
      181 atagataccc tcacctccct gcgctttttc gcggcggcgg ccattgtcgt ttatcacagc
      241 cgcggccact tcggtatccc cagtgattat ctgtccccct tcgtcctcga ccagggagtc
      301 agttttttct tcgttctgtc cggcttcatt ctgagcctgg tctatggtga gaagctgaaa
      361 gagggcggac tcaatgccgg tagtcgcctt gcagacatcc tatcttttat tgtggcgcgc
      421 tttgcccgca tctggccctt gcacgtggcc accctggtgc tggttgcttt tcttttaccc
      481 gagaaatgga accacgatct gagcccggtt aatttgcttg cttccctctg cttgatgcaa
      541 tcctggattc ccatcaacga tgtcagtttt gcctttaacg gtgtcagttg gagtctgtcg
      601 gcggagttgt ttttctatct ctctttcccg tttttgatag tggcttacga gaagaactgg
      661 ctgagaaact cgctactagc ccttgggctg atgacttctc tgtcctgcct ttgtgcaatt
      721 tgcggattcc cagaacacgt tggaaatggt gtcagcatgc atgaactcat gtatgtgagc
      781 ccgctttcga gggtgtttga gtttagcctg ggaattgggg cctttgctct ggccaggcgt
      841 tggcacatta actggcgcgc cggcgagtcg gcagcggccg cctcgtcctt caagttacgg
      901 ttggtcttca ccattattga actagccgta cttttgattg ggcttttcgt tgcctacaag
      961 agcacccgca tctcatcttt tctggccgaa gccatggaca gcttgctgcc cggagtttct
     1021 gctccactaa atacgtggct catgcatggt ggttccgtgg ccctggtctt tgccctgatg
     1081 attatcgttt tttccttcgg tcggggactc ttaagtaagg tgctgagctg gcctttgctt
     1141 gtctacctgg gcgagatcag tttttctgtt tacctttcgc accgcctgct cttgcattac
     1201 tattacaatc actttgccac tgtagatggt gcaatgccga tcgccgttta ttggctggta
     1261 cttctcagtg tcagccacct gctctacgtt tttgtggaat gtccctgtcg cagtcttatt
     1321 gttggtctct ttcgccgttt ccagaaaggg gcgagaccgg agggtgtttc ctcttcctgg
     1381 tcacagattt tgcctcagtt tcgcttgagc cttttgaacc gttttttcct ggcggcagaa
     1441 gcgctttttc ttctggcgct gccggcgctc ttgttctccg gaccggcttt agcccggctc
     1501 gagggctctc aactgacgcc aaccgtcttg gatagtgtaa gtaaacccct ggccaggctg
     1561 ggcgatgatg tggaactatt gagcgtgagc ggccgtgatg atatcggcgg caagcggaag
     1621 gtcatactga cctggcgcgc tttgcggcac cagaaactga atggttttgt gacgctgcat
     1681 ctatacacgc ccgacaatgt ctactacaag cgccgcgatt acaagcgcgc ctggcgggaa
     1741 gaggaagtag cgagcggcca gatctggcgg gatgaagtgc tcctcaaccg ggaggaaact
     1801 gctaccacc
//
LOCUS       DAOQNZ010000932         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46583_length_1809_cov_3.099772, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000932 DAOQNZ010000000
VERSION     DAOQNZ010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46583_length_1809_cov_3.099772"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PKW73_14990"
     CDS             <1..564
                     /locus_tag="PKW73_14990"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HNA74651.1"
                     /translation="IYLEPVPRFRLHLPLITNSDCFIVSPDINVHFKAGYLWKLDPKS
                     TIHSACNFGKEPRIHLMLDCYVNQTLAELIEKQFLDEDAKFVKPALTKTIKEDLQEQA
                     INLLKQSPDNLTAAEEIILAAFCRYNLYRLKEGLTTYDILSQVYEQATRAIPTLAALL
                     KERQAYWQARLVETYPERKEKEAALAV"
     gene            572..>1809
                     /locus_tag="PKW73_14995"
     CDS             572..>1809
                     /locus_tag="PKW73_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74652.1"
                     /translation="MKEGKLHFQIHDAGRGRSFSLPSVQDYQEEKRIKVVAASPLNFG
                     PEADKGAGTCSHHEFIAHRLEAALALLSNTPSYFPGQTIPGPGSMGLEVNRGNLLIEQ
                     FRHLLNFDEKACARLLQTGNDSYSLYSVKVPAKEVWRFNLIEKPSLSFLYQAEEPARL
                     KSPDIPARAYQVLKTDLIEHYAKLKADDCPENLQGALKAVARAWHKEDGRRDLSKTAL
                     TAAYKLWQHYSEQGSVNEGNFMAFAFELEALAFHQTAKEKLGLLSFAGGVFADNYVQI
                     ALMPLAKIQATEFCVRERLFDEVINLVGRGFAPVVVNEHGLVADGNHRVTAAWIWNFL
                     KYTAGNCKTNAKQATWSLDDPRFQREAANFFLQSSVTGAISDVSRHEILSHLAAYLSN
                     PECRAKLQTYIKPQLSKYEYV"
BASE COUNT          493 a          493 c          437 g          386 t
ORIGIN      
        1 atctatctcg agcctgtacc gagattccgc ctgcacctgc ctttgatcac taactccgac
       61 tgctttatcg tcagccccga cataaacgtg cacttcaaag ccgggtatct ctggaaactc
      121 gatccgaaaa gcaccataca tagtgcctgc aatttcggca aagaaccgcg tattcacctg
      181 atgctcgact gctatgtcaa ccagacactg gcggaattga ttgaaaaaca gtttcttgat
      241 gaagacgcca aatttgtcaa accagcactc acaaagacca taaaggaaga tctgcaagag
      301 caagccatca acctgctcaa gcaatcgccg gataatctga cggcagcgga agaaatcatt
      361 ctagcggctt tttgtcgcta caacctctac agactgaaag agggtctcac tacctacgac
      421 atactgagcc aggtttacga acaagcgacc agagccattc cgacactggc tgccctgctg
      481 aaggagcgtc aggcctactg gcaagccaga ttggtggaga cctatcccga acgcaaagag
      541 aaggaagcag cactggctgt ttgaaaaaaa catgaaagaa ggcaaattgc attttcaaat
      601 tcatgacgcc gggcgaggca gaagcttcag cctgccctcc gtgcaagact atcaggaaga
      661 gaagcgcatc aaggttgtgg cggcaagccc actcaatttt ggaccggaag ccgataaggg
      721 cgccggcact tgcagccacc acgaatttat cgcgcaccgg ctggaagcag cgctggctct
      781 gctttccaac acaccatctt actttcccgg tcagaccatt ccgggtcccg gctctatggg
      841 tctggaagtg aaccgcggca acctgctcat tgaacagttc cgccaccttc tcaacttcga
      901 tgaaaaggct tgtgcaagac ttctacaaac tggaaacgac agctacagcc tctactctgt
      961 aaaggttcct gcaaaagaag tctggcgttt caatctaata gaaaagccca gcttgagctt
     1021 tctctaccag gcggaagagc ctgccagatt gaaatctcct gatattccgg caagagccta
     1081 ccaggtgcta aaaacagatc taatcgaaca ttatgcaaag ttgaaagccg atgattgccc
     1141 ggaaaatctg caaggggcgc tgaaagcagt ggcgagggcc tggcataaag aagacggacg
     1201 gcgggactta agcaaaacgg cacttaccgc cgcctataag ctctggcagc actacagtga
     1261 gcaagggagc gtcaatgaag gtaacttcat ggcttttgcc tttgaactgg aagccctggc
     1321 ctttcaccag accgccaaag agaagctggg tcttttgagt tttgccggcg gcgtcttcgc
     1381 cgacaactat gtgcagattg ccctgatgcc cctggctaag attcaagcca ccgaattctg
     1441 tgtgagagaa agactctttg atgaagtaat caatttagtt ggtcgcggct ttgctccggt
     1501 ggtggttaac gaacacggtc ttgtggccga cggcaatcac cgggtgactg ccgcctggat
     1561 ctggaatttc ctcaagtaca cagccggcaa ctgtaagaca aatgccaaac aggccacctg
     1621 gtcccttgac gatccccgct tccagagaga agctgccaac ttcttcctgc agtcatcagt
     1681 aacgggagcc atatccgacg tttcccgcca tgagatttta agccacctgg ctgcttacct
     1741 ttccaacccc gaatgccggg caaaactgca aacctatatc aagccgcagt tgtccaagta
     1801 cgagtatgt
//
LOCUS       DAOQNZ010000933         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46591_length_1809_cov_2.769099, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000933 DAOQNZ010000000
VERSION     DAOQNZ010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46591_length_1809_cov_2.769099"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKW73_15000"
     CDS             <1..581
                     /locus_tag="PKW73_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74653.1"
                     /translation="ANKDGHFHRSLKPGNIIFIDDMNSEPAVAVTDFSLGKLKLDASA
                     TPFDAFYMSADEARGLDFDERSQLYTLGLVGFALLTGVPPFQMGSYAEIKNKHALELP
                     PQIASLKFDAARPRDLEEVIERCLDKDPRNRFDSVAKLVERLEVFPAREKARIAAILA
                     ARKRRKLLQITAVVLGIVLVLCAIAYFVLGRH"
     gene            complement(664..>1809)
                     /locus_tag="PKW73_15005"
     CDS             complement(664..>1809)
                     /locus_tag="PKW73_15005"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNA74654.1"
                     /translation="RLVPHATLSIEAKQQMNNLRKKGVTVQPVSIEGRKIATTFWGTA
                     WCNHLEKFSDYANRLPRGRTYVRNGSVCHLEIAQGQIKAIVSGSSLYDVLIKIEPLPE
                     HKWTEVKKRCAGEISSLLELLKGKFSTAVMTAVTEKNTGLFPLPTEIQLDCSCPDWAT
                     MCKHVAAVLYGVGARLDSQPELIFTLRAVDQTELIAESTDLMQGDDKSGKSSRFVDDD
                     LGAVFGIEFADEPTGKAKQISSKSQTVSSTPATPKQSKEPEPISKHKSRTSPVKKTTP
                     VKPVKAVKQTVKKDQPIGKPSSPSGKDAVNFDFSKSPTARQVRELRKTLNSTVGEFAN
                     ILGVSYSTVANWEKKRGKLNLSTRSKEELQAVAKLASKKKPPQVRKS"
BASE COUNT          357 a          438 c          483 g          531 t
ORIGIN      
        1 ctgccaataa agacggccac tttcatagaa gtctcaaacc gggcaacata attttcattg
       61 acgatatgaa ctcggaaccg gccgttgccg ttacagactt tagccttggc aaattgaagc
      121 tcgatgcttc tgcgactccc ttcgatgctt tctatatgtc tgccgatgaa gcccgtggtc
      181 tcgatttcga tgaacgatcg cagctctata cactggggct ggtcggattc gcccttctga
      241 ccggcgtgcc gccgtttcag atggggtctt acgccgagat caagaataag catgcccttg
      301 aattgcctcc ccagattgcc tcactaaaat ttgatgcggc tagacccagg gacttagagg
      361 aagtgattga gcgttgtctt gataaagatc cccgcaatcg attcgattcg gtggccaagc
      421 tggtggagcg cctggaagtc tttcccgccc gtgagaaagc tcgtatagcg gcaattcttg
      481 ccgcccggaa gcgccgcaag ctcttgcaaa ttactgccgt ggtattgggg attgtgctgg
      541 tgctttgcgc tatcgcctac tttgttctgg gccggcattg agagcgattg aggaagccta
      601 tgtggctttc ttgaattatc tatgttttga gattcataaa tggtttcatt cgctgcgagc
      661 gagtcaagac tttctgacct gtggaggctt tttctttgaa gctagtttcg ctactgcttg
      721 tagttcttct ttggaacgag tgcttagatt gagtttgcct cgctttttct cccaattggc
      781 tactgtcgaa tagctgaccc caagaatgtt ggcaaattca cctacggtcg agtttagggt
      841 cttgcgtagc tctctaacct gccttgctgt gggagatttc gagaagtcga aattgacggc
      901 atcttttcca gaagggcttg atggttttcc gattggctgg tctttcttga cggtttgctt
      961 gactgcctta accggcttca caggcgtagt cttcttaacc ggactagtcc tgctcttgtg
     1021 cttcgagatt ggttcaggct ctttcgattg tttgggtgtt gcaggtgtgg agctgaccgt
     1081 ctgtgacttt gagctgatct gttttgcctt tccggttggt tcatctgcaa attcaatacc
     1141 gaagacagcg cccaagtcgt cgtcaacaaa gcgggaactc ttgccggatt tgtcgtcacc
     1201 ttgcattaaa tctgtgcttt ctgcgataag ttcggtttga tcgacggcgc gcagggtgaa
     1261 aatgagttcc ggttgactat caaggcgcgc acccaccccg tagagaacgg ctgctacgtg
     1321 cttgcacatt gtggcccagt cggggcaact gcaatccagc tggatttctg taggaagcgg
     1381 aaatagtccc gtgtttttct cggttacagc tgtcattacg gctgtagaga acttcccctt
     1441 gagcagttca agaagtgagc ttatctctcc agcacagcgt tttttgactt cagtccattt
     1501 atgttccggc aagggctcaa tttttataag cacatcatag agggaggaac cgctgacaat
     1561 ggctttgatt tggccctgcg cgatctccag gtggcagacc gagccgttgc gtacataggt
     1621 tctgccccgg ggtagccgat tggcgtagtc gctgaatttc tctaaatggt tacaccaggc
     1681 ggtcccccag aaggtcgtgg cgattttgcg accttctatg ctcacgggtt gaaccgtaac
     1741 tccttttttt cggaggttgt tcatctgctg cttcgcctcg atgctgaggg tggcgtgcgg
     1801 cacgaggcg
//
LOCUS       DAOQNZ010000934         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46629_length_1808_cov_4.293212, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000934 DAOQNZ010000000
VERSION     DAOQNZ010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46629_length_1808_cov_4.293212"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKW73_15010"
     CDS             complement(<1..494)
                     /locus_tag="PKW73_15010"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HNA74655.1"
                     /translation="MGTLSTLSNTATIPTEEEAYAIALESYFYFYPMMSMEATRKQCT
                     NLPVGARPGFGPANLFSHMRAYPEADFRAVVRPNFDTLYSVVWLDLRQEPQVVTTPDT
                     KGRYYLLPMLDMWSDVFAAPGWRTSGTKEQKYLITTRGFQGNVPRDLKVIEAPTPFVW
                     VIGR"
     gene            complement(568..1068)
                     /locus_tag="PKW73_15015"
     CDS             complement(568..1068)
                     /locus_tag="PKW73_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74656.1"
                     /translation="MKAENFEERDVTAEPALGKKLAGEIWNDKLAAEHLLLTDRKQGQ
                     PSKHLNETQIADGGGLYIKFSEGRGLSEVGGYVTTRAGGGEMTLMGGIELKEGNTNEY
                     LLGFDYKEREKHPKRERSQKPEHPSADNPTEPKPRPEEPGNHNDKPDSPNRPVRPHQP
                     PHKATN"
     gene            complement(1167..>1808)
                     /locus_tag="PKW73_15020"
     CDS             complement(1167..>1808)
                     /locus_tag="PKW73_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA74657.1"
                     /translation="TSIKVMVVDDQPMVRKGIVSLLQLEGGIEVVGEAGSGAEALSLV
                     PRLSPELILMDVRMPNMDGIECTQKILKAYPNIKVLILTTFDEDEYIFPAIEAGCCGY
                     LLKDTPLEDLSAAIKIVHKGNVQLGPSIAPKVLARLRPYKERGETEKLRFMTEREKTI
                     LKLIGQGNSNKEIATTLNISEGTVKNHISSILTQLGLRDRTQAALWVAENGIG"
BASE COUNT          371 a          444 c          459 g          534 t
ORIGIN      
        1 atgcggccga ttacccagac aaagggtgta ggtgcctcga tgactttaag gtctctgggc
       61 acattgccct ggaagcccct ggtggtgatc agatactttt gctctttggt gccgctggtg
      121 cgccagcccg gcgctgcaaa aacatcgctc cacatgtcca gcatgggcaa tagataatag
      181 cggcccttgg tgtcaggcgt cgtaacaact tgaggctctt gcctgagatc gagccagacc
      241 acggaataga gagtgtcgaa attaggtctc acaactgccc ggaaatctgc ctccgggtag
      301 gctctcatat gagagaacaa attggccggt ccaaagcccg gtcttgctcc caccggcaga
      361 ttggtgcatt gcttgcgggt ggcttccatg gacatcatgg gataaaagta aaaatatgac
      421 tccagagcta tggcataggc ttcctcttcg gtcggaatgg tagccgtatt gcttaatgta
      481 gagagcgtgc ccatgattca tctcctgccg aatatatgaa agacatcgga aggttagcac
      541 atagaagggc agtcaggtat tacgcgatta gttggtcgct ttatgtggag gctggtgagg
      601 tcgcactggt ctattcggac tgtccggctt gtcattatga ttgcccggtt cttccggtcg
      661 tggttttggt tctgtcgggt tatccgcgga tggatgttca ggtttttgag agcgctctct
      721 tttgggatgt ttctctcttt ccttgtaatc gaatcccagt aggtattcgt tagtgtttcc
      781 ctctttcaac tcaatgccgc ccatgagcgt catttcccca cctccagctc tggttgtgac
      841 atagccgccc acttctgaga gaccacgccc ttctgagaat tttatgtata gtccgccgcc
      901 gtcggcgatt tgtgtctcgt tcaggtgttt agagggttgc ccctgttttc gatctgtcaa
      961 tagtaagtgc tcggccgcaa gtttgtcatt ccagatttct ccggcgagtt tttttcccag
     1021 ggcgggttct gcggttacgt cgcgctcttc aaaattttct gctttcattt tgcaaatgct
     1081 cctgcattga cctgtaaaga cacagtcaga agcgtatttg gccgattctc aggcagccag
     1141 tgagaaaagt catgttgttg aataattcat ccgataccat tttctgctac ccatagcgct
     1201 gcctgggtgc ggtctctcaa acccagttgg gttagaatgc tgcttatatg attcttgacg
     1261 gtaccttccg agatgttcaa ggtcgttgca atttccttat tggaattacc ctgacctatg
     1321 agttttagaa ttgtcttttc tctctctgtc ataaagcgga gcttttcagt ttcgcccctt
     1381 tctttgtaag gacgcagtcg cgccagcacc ttgggcgcaa ttgaggggcc aagctgcaca
     1441 ttgcctttgt gcacgatttt gattgctgcc gaaagatctt caagcggtgt gtcttttagt
     1501 agataaccgc aacagccggc ctcgatggcc gggaatatgt attcatcttc gtcgaaggtg
     1561 gtgagaatca gaactttgat gtttgggtag gccttcagga ttttctgcgt gcattcgata
     1621 ccatccatat ttggcattcg cacgtccata agaattagct ccgggctcag tcttggaacg
     1681 agggagagtg cctcggcacc acttccagct tcgcccacaa cttctatgcc gccttccaat
     1741 tgtagaagtg aaacgattcc ctttctcacc atgggctggt catccactac cattactttg
     1801 atgctggt
//
LOCUS       DAOQNZ010000935         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46637_length_1808_cov_3.624643, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000935 DAOQNZ010000000
VERSION     DAOQNZ010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46637_length_1808_cov_3.624643"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKW73_15025"
     CDS             <1..775
                     /locus_tag="PKW73_15025"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HNA74658.1"
                     /translation="FFQEVMDVVNGLHDFLEEDPELKALAKTHGRRLFDVRKSPEKLP
                     VARGLVMETDAYVVLTVGTDCSVGKMTVALEIQKLAQSKNIDAQFVATGQTGIMICGE
                     GIAIDRVIGDFMAGATEKMVLEKAARGNLILVEGQGSLAHPGFSGVTLALLHGACPQA
                     LILCHRPARPTIKGTNFPISSYSRLIETYEAMASYMRPARVIALALNTQDLNQEEALA
                     AIKKAEAETGLPAADPVRFGAHVLLDAIEKAGILSPIGK"
     gene            806..>1808
                     /locus_tag="PKW73_15030"
     CDS             806..>1808
                     /locus_tag="PKW73_15030"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNA74659.1"
                     /translation="MTTKTKCINLSFAPLNLTTRHPFGISYGTSSQTLNVQVQLELDG
                     LIGLGEAAPASYHGESRETVMAVLAKFQAHQEELLGNSAHAIHAVSTRLDKMIAGHSA
                     AKAAIEMAMHDLAGKVSNLPTWKMLGLADAQAPMTDFTIGIDNLEMIEKKTEEAVAAG
                     HKMLKVKLGTSYDRQIIEKVRKVAPNLPLRVDANGGWSVKQAVDCARFLHEHQVEFIE
                     QPLPKFALKEDFQFVKDHSPVPIFADESVCKASDVARLAGAIDGVVVKLAKSGGLLEA
                     MRVIATARAHGLKIMFGMMIESSIGVTAAAQLSSLCDYLDLDGALLLKDDPYEGALWQ
                     "
BASE COUNT          461 a          527 c          473 g          347 t
ORIGIN      
        1 cttcttccag gaagtcatgg atgttgtcaa cgggctccat gacttcctgg aagaagaccc
       61 ggaactaaaa gcacttgcta aaacccacgg aaggcggctt ttcgatgtgc gaaagagtcc
      121 cgagaaattg ccggtagcca ggggtctggt catggaaacc gacgcctatg tagttctcac
      181 cgtcggcacc gactgctccg tgggcaaaat gaccgtagcc ctggagatac aaaaactagc
      241 ccagtcaaaa aacatcgatg cccagtttgt ggccaccggt cagaccggca tcatgatctg
      301 cggcgaaggc attgccatcg accgagtcat aggcgacttc atggccggag ccacggaaaa
      361 aatggtgctg gaaaaggcag cccggggcaa cttgattctt gtggaagggc agggctccct
      421 cgcccatccc ggcttctccg gcgtcactct ggcactgctg catggagcct gcccccaggc
      481 cttaatcctc tgccatagac cggcaagacc gactattaaa ggcaccaact tccccatatc
      541 aagctactct cgcctgattg aaacctatga agccatggcc tcctatatgc gtccggccag
      601 agtaatagcc ctggctctca acactcaaga tctgaaccag gaagaagctc tggcggccat
      661 caaaaaggcg gaggcggaga ccggcttacc ggcagccgat cccgtgcgct tcggagccca
      721 tgttttgctc gacgccatcg aaaaagcagg catcttgagc ccgatcggga aatagaaaca
      781 acaagaacta aacaggacaa aaaaagtgac cacaaaaacc aagtgcatca atctctcttt
      841 cgcacctctg aacctgacta cccgtcaccc cttcggcatc tcctacggca cttcttctca
      901 gaccctgaac gtgcaagttc aactggagtt ggatggcttg atcggtctgg gagaggcagc
      961 gccggcttct tatcatggcg aatcgaggga aacggtcatg gccgtcctcg ctaaatttca
     1021 ggcacaccag gaagaactgc tgggtaattc cgcccatgcc atccatgccg tttcgacccg
     1081 cctggacaag atgatcgccg ggcacagcgc cgccaaagcg gccattgaaa tggccatgca
     1141 cgatctagcc ggaaaggtga gcaatttacc cacctggaaa atgcttgggc tggccgacgc
     1201 tcaggcccct atgacggact tcaccatcgg catcgacaac ctggagatga tcgaaaagaa
     1261 gaccgaagaa gcagtagccg ccggtcataa aatgctcaag gtcaaactgg gcacaagcta
     1321 cgaccgccag atcatcgaaa aggtaagaaa agtcgctccc aatctgcctc tgcgcgtaga
     1381 tgccaatggc ggctggagcg tcaagcaagc cgtagactgt gcccgtttcc tccatgaaca
     1441 ccaggtggaa ttcattgaac aaccgctgcc caagttcgcc ctgaaagaag acttccaatt
     1501 cgtgaaagac cactcaccgg tgcccatttt tgccgatgaa agcgtctgca aagcctcgga
     1561 tgtggccaga ctggccggcg ccatagatgg tgtcgtagtc aaattagcca aaagcggcgg
     1621 tctcctggaa gccatgcgcg tgattgccac agccagagcc catggtctca aaatcatgtt
     1681 cggcatgatg atcgaatctt ctatcggcgt gacggcagcg gcgcaacttt ccagcctgtg
     1741 cgactacctg gatctggacg gtgcccttct gctcaaggac gacccctatg agggcgcctt
     1801 atggcagg
//
LOCUS       DAOQNZ010000936         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46652_length_1808_cov_3.102681, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000936 DAOQNZ010000000
VERSION     DAOQNZ010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46652_length_1808_cov_3.102681"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKW73_15035"
     CDS             complement(<1..582)
                     /locus_tag="PKW73_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74660.1"
                     /translation="MSPDRTLRFNNMKDAHAALTSGASFAIVEMALQEDEEIILNMAM
                     EKMFDVDAFACIDQNSLPFYPATLEGRVLMLISELTRKAPRLLDHERRATYSFLHRTI
                     LACAEIGTIEDQTHVRGTISEDGSKGVDQWFHLDCLPESVAPESEQYLALVTLGDYPG
                     TQLVLPGEATRQELDRLARLKENTIGRIKERGFK"
     gene            complement(629..1222)
                     /locus_tag="PKW73_15040"
     CDS             complement(629..1222)
                     /locus_tag="PKW73_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74661.1"
                     /translation="MNNSVDTKSALFTVAKIYLAADILWATVFATFTGSLLSSGVDLA
                     NCGPSELGQATPGLVAGTLLAFMAALFQGAIFLIVFTGFGIAISLAVKKIFKTEAKVL
                     WQIGRWVLGALCGWALLASTVLNSFFNLGLGMVGSLLGCTSAIHLGFFGSAAAWIITF
                     VSAFIQGTIFLLVGSGFVFFFLVVISLVLGKKNQDSK"
BASE COUNT          453 a          453 c          453 g          449 t
ORIGIN      
        1 tttgaagccg cgctctttta tccggccgat tgtgttttct ttcaggcgtg ccaatctatc
       61 caactcttgt ctggttgctt caccaggcag gaccagctgg gtgccaggat agtcgccgag
      121 ggtaacaaga gccagatatt gttcactttc gggggcaacg ctttccggca ggcagtcaag
      181 atgaaaccac tgatcaacgc ctttgctgcc atcctcgctg atggtacctc gcacatgggt
      241 ttggtcctct attgtgccga tttccgcgca tgcgagaata gtgcgatgga gaaagctgta
      301 tgtcgcccga cgttcatggt ccagcagtct cggagccttt ctcgtcagct cgctgattag
      361 catcaagacc ctgccttcca gcgtggctgg ataaaacggc agcgagtttt ggtcaatgca
      421 ggcaaaagca tccacatcaa acattttctc catcgccata ttgaggataa tttcctcatc
      481 ctcctgcagt gccatctcca ctatggcgaa ggacgcgcca ctggttagag ccgcatgagc
      541 atccttcatg ttgttgaatc tcaatgttct gtcaggtgac atggtagttc tcccgatcaa
      601 tcagcgactg aagagagccg ctagaaagtt actttgaatc ctggtttttt ttgcctaata
      661 cgagcgatat caccaccagg aagaagaaga caaatccgct gccgaccaag agaaaaatgg
      721 tgccctgaat gaaagcgctc acgaaagtga tgatccaggc tgcagctgag ccgaaaaagc
      781 cgaggtgaat ggcgcttgtg cagcccaaaa gtgagccgac catgcccaga cccaggttga
      841 agaaagagtt gagcacggtg gaggcaagca atgcccagcc gcaaagagcc cccagcaccc
      901 agcgtccaat ttgccagaga accttggctt cagtcttgaa gattttcttg accgccaggg
      961 atatggctat gccaaagccg gtaaacacga tcaggaaaat tgctccttga aagagggcgg
     1021 ccatgaaagc caataacgtg ccggccacaa gaccaggcgt tgcctgtcct agttcggagg
     1081 gtccgcagtt ggccagatct accccgctac tcagcaatga gccggtaaaa gtagcaaaga
     1141 cagttgccca gagaatgtcg gcagccagat agattttggc tacggtaaat aaagcagact
     1201 ttgtatcgac tgaattgttc atagtatttg tcctgaagtt aggtggattg aaaatgactg
     1261 caacgctata agcccccgtg gcagcgaact gccgggtgat gtttccttga tctgtgctta
     1321 cgttcttttc ttgagagttc ttttgagggc taattgaagt tgcaacttca acctagtgaa
     1381 ttagctgttt cagtcaaagg ctctctttct ctctgcttca ttatttggcc gggaatctcg
     1441 tagaagccgc aattaaaaga gctccacgag aattagccag gtctcaccgt ccctttgctg
     1501 gagcagaaag cgtactgttt tgtcggttgc gccagcattg acgtccgaaa ggactctcag
     1561 tggcacggta gaagaggatt tagaaactcc tacctcttcg atgcttgcta catggcttag
     1621 tgaatcaatg ccaaaaaaat caaaaaaccc attctgcttt gacaggttgt gaaagttgtt
     1681 caatgttgct ttcatcgtag gtcgaacttg tagacacccg ttttgcctac acactcaggt
     1741 gaccagccat cgccgatggt gtggacctcc ctgtccacaa atcagccatc cttagggaag
     1801 gggtttca
//
LOCUS       DAOQNZ010000937         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46700_length_1807_cov_3.204909, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000937 DAOQNZ010000000
VERSION     DAOQNZ010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46700_length_1807_cov_3.204909"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKW73_15045"
     CDS             <1..189
                     /locus_tag="PKW73_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74662.1"
                     /translation="QPVLVLLIENGATKEVSEAVFLHHSTRVPVGWGTYKDGRPSALV
                     FFQGLTENQGLSEDLFHL"
     gene            205..921
                     /locus_tag="PKW73_15050"
     CDS             205..921
                     /locus_tag="PKW73_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74663.1"
                     /translation="MNNGSTERSKTLFALTAAALFLVMAPLKAGADETGKDDKPADEG
                     TAKASKLSKSPRAGTRYSPSEFLSDLRDTRLTLGQIKQQAVNLFLEATRPEVSRDARL
                     IEHSPLAITPSMYISAKKYQEPRKEWLVFYINTLEPILQLLNEDLKDAEENGLRLPDG
                     LDKKITPLWKPWKQEVLGIDKAMDDLQEQIGQANNNAALAKTALTIFNQVSKLEKIRY
                     QAAQICFSEIARSKSVGKAP"
     gene            complement(923..>1807)
                     /locus_tag="PKW73_15055"
     CDS             complement(923..>1807)
                     /locus_tag="PKW73_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNA74664.1"
                     /translation="FAPRGAALEVHSPAFSFTLNDKRQVWAAEVADLYDQAVANQWDG
                     VRDIDWSSLPPLPPLLEQAVCQIMTFLVENEYSALYVPGKFISRINPQYMEVVLFLTT
                     QLMDEARHIEVFTKRALANGGGLQVCTASTEHSLKCLFEQSDFSCASFLLSVLGEGTF
                     VDLLRFIEEVAPDPVTADIVRRARIDEIRHVHFGMAHIRYLLSEDAGAVNMLMRAVFE
                     RSLVLRDVTSINELVTRALAILAGGGIEPAQLKVGTGRVNGLLKLMDENRRKRLVSIG
                     FSPEQAAEVSALHTPNFM"
BASE COUNT          480 a          493 c          463 g          371 t
ORIGIN      
        1 caaccagtgc tggtactttt gatagaaaac ggcgccacaa aagaagtttc cgaggccgtt
       61 tttctgcacc attccacacg ggtgccggtg ggctggggaa catacaaaga cggaagacct
      121 tccgccctgg tcttcttcca ggggctgaca gaaaatcagg gtctctccga agacctcttt
      181 cacctttagt ttgaggagtt accaatgaac aacggttcaa ccgaaagaag taagactctc
      241 tttgccctga ccgcggcggc tctgttcctg gttatggccc ccctgaaggc cggcgccgac
      301 gaaacaggaa aagacgataa accggcagat gaaggcaccg ccaaagcctc aaaactatcc
      361 aagtctcccc gggccggcac cagatattca ccttctgaat ttctttctga cctgcgcgat
      421 acacggttga ctctcggaca aataaaacag caggctgtca atctgtttct ggaagccacg
      481 cgcccggaag tcagccggga cgctcgtttg attgagcact cgcccctggc aatcacgcct
      541 tccatgtaca tttcagcaaa gaagtaccag gagccgcgca aagaatggct ggttttctat
      601 ataaacacgt tggagccaat cctgcagctt ctcaatgaag acttgaaaga cgccgaggag
      661 aatggtctga gattaccgga cggactggac aaaaaaatta ctcccctctg gaaaccatgg
      721 aagcaagagg tgctaggcat tgataaagcc atggacgact tgcaggagca aattggacag
      781 gcaaacaata acgccgccct ggccaaaaca gctctcacca tcttcaatca agtcagcaaa
      841 ctggaaaaga tccgctacca ggcagcccag atatgttttt cggagatagc ccgctccaaa
      901 tccgtgggca aggcgcctta acttacataa agttgggagt atgcaaggct gaaacttctg
      961 ctgcctgctc gggactgaag ccaatgctga cgaggcgttt gcgcctgttt tcgtccatca
     1021 gcttgaggag accgttgacc ctaccggtgc ccactttcaa ttgcgccggt tcgatgccgc
     1081 cgccggccag aatagcaaga gcgcgggtga ccagttcatt gatggaagta acgtcgcgca
     1141 gtacgaggga acgctcgaaa acggcgcgca tgagcatatt caccgccccc gcatcttcgg
     1201 acagtagata acgaatatgt gccataccga agtgcacatg ccttatttca tcaatgcgag
     1261 cccgccttac aatatcggca gtgaccggat ccggcgccac ctcctcgata aatctgagca
     1321 aatcaacaaa ggtgccttcc cccagtacgc tcaagaggaa ggaagcgcaa ctaaagtcgc
     1381 tctgctcaaa tagacatttg agtgaatgtt cagtggatgc ggtgcatacc tgcagcccac
     1441 cgccattagc cagggcgcgc ttggtgaata cttctatgtg ccgggcttca tccatgagct
     1501 gcgtggttaa gaaaagcact acttccatat actgaggatt gatgcgggaa atgaatttac
     1561 ccggcacgta gagtgccgaa tattcatttt ccacaagaaa ggtcatgatc tggcaaacgg
     1621 cttgctccag gaggggagga agtggcggca aactgctcca gtcaatgtcg cgaacgccgt
     1681 cccattgatt ggccacggcc tgatcataaa ggtcggctac ctccgccgcc cagacctgcc
     1741 gcttatcatt caaagtaaag gaaaaggcgg ggctatgcac ttccagagcg gcgccacgag
     1801 gggcaaa
//
LOCUS       DAOQNZ010000938         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_46983_length_1802_cov_3.120778, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000938 DAOQNZ010000000
VERSION     DAOQNZ010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46983_length_1802_cov_3.120778"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKW73_15060"
     CDS             complement(<1..492)
                     /locus_tag="PKW73_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74665.1"
                     /translation="MSEIHKLKQALLEGKPLPRLTGCYGRIIRTRYDRPLALAEENER
                     RAVMVMGPSGLKHILLRPGYEMLLNIGYTAGYIKTKLDKGFRFELLIFKLRREELKVA
                     SWKNVLTMACTCYPEAAHLFREHAREMKAKSIAEFEALAGFSFAEVDNLGAEDPRYMT
                     MDRL"
     gene            complement(548..1546)
                     /locus_tag="PKW73_15065"
     CDS             complement(548..1546)
                     /locus_tag="PKW73_15065"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA74666.1"
                     /translation="MMTGIPVAALDVEETSRLLTMEEHLHKRVIGQNEAISAVSRAVR
                     RGRAGIKDPRRPTGMFLFLGPTGVGKTELVKALQDFLSNNQDDLIRLDMSEFMERHSA
                     AKLIGAPPGYVGYEEGGKLTEAVRRRPYGVILLDEIEKAHPDVFNLLLQISDAGRLTD
                     GQGRTVDFKNSIIIMTSNIGARAIQNAIRSKSRAPTDEIMEEVRQVFSPELINRMDEI
                     ICFHPLSRQEQEEILQLIIGEIKDRLPPGLSLEMTDEARDILIAQGYDSLYGARPLRR
                     TVTRKLEDPLADLIISGKPASGDTLMVETDDDLNINIRVKPEPSKTVVLVKEEPAD"
     gene            1545..>1802
                     /locus_tag="PKW73_15070"
     CDS             1545..>1802
                     /locus_tag="PKW73_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74667.1"
                     /translation="MLTTAWIAICDIVAMIALPLATASGDTETVAIGVAVVGVVAIAC
                     LVGLRFFRSSDIGEHLRQLSRSKHWALDLRLSLAMLFGLAWL"
BASE COUNT          325 a          477 c          507 g          493 t
ORIGIN      
        1 gagcctgtcc atggtcatat agcgcgggtc ttcggctccc aggttatcca cttccgcgaa
       61 gctgaatccg gccagggctt cgaattccgc gatcgatttt gccttcattt ccctggcgtg
      121 ctctcgaaac aagtgagcgg cttccgggta gcaggtgcaa gccatggtga ggacattttt
      181 ccaggaagca actttcagtt cttcccggcg cagcttgaag atgagaagct caaagcgaaa
      241 gcctttatcc agcttggttt tgatgtagcc tgccgtatag ccgatattga gcagcatctc
      301 gtaacccggt ctgagcagaa tgtgcttcag accgctcgga cccatgacca tcactgcccg
      361 gcgttcgttt tcttccgcca gggccagagg tcggtcgtaa cgggtgcgaa tgatacgacc
      421 gtagcaaccg gtcagtcgtg gaaggggctt gccttccaag agcgcttgtt ttagcttgtg
      481 aatttcagac ataggcattt acagcactcc cagtgtgctt ggcacaaagt ttggtttgaa
      541 atggagttca gtccgcaggc tcttctttaa ccagcaccac ggttttagac ggttccggct
      601 tgactcttat gttgatgttc agatcgtcat cggtttctac catcagggtg tcgccgcttg
      661 ccggcttgcc tgagatgatt aagtcagcca gaggatcttc cagttttctt gtgacggtgc
      721 gccgcagtgg tcgggcaccg tagagagagt cgtagccctg ggcgatgaga atgtccctgg
      781 cttcgtcggt catttccagg ctgaggccgg gtggcagccg atctttgatc tcccctatga
      841 tcaattgcaa aatttcttct tgctcttgcc tgctcaaagg gtggaagcaa attatttcgt
      901 ccatgcgatt gatcagctcg ggtgagaaga cctgcctgac ttcctccatg atttcgtccg
      961 tgggggctcg gcttttgctt ctaattgcgt tttgaatagc gcgagcgcca atgttggagg
     1021 tcatgatgat gatcgagttc ttgaagtcta cggtgcgtcc ttgcccgtcc gtgagcctgc
     1081 cggcatcact gatttgcaag agcaagttga agacgtcggg atgggccttc tcgatttcat
     1141 ccaggaggat gactccgtat ggtctacgcc gaactgcttc cgttagcttg ccgccttctt
     1201 cgtagcccac atagccgggt ggtgctccaa ttagtttggc ggcggaatgg cgttccatga
     1261 attccgacat gtccaggcgg atcaggtcgt cctgattgtt ggaaaggaag tcttgcagtg
     1321 cctttaccag ctccgtcttg cctaccccgg ttgggccgag aaagaggaac atgccggtgg
     1381 gtctgcgtgg atcttttata ccggctcgtc ccctgcgaac agctctggac acggcgctaa
     1441 ttgcctcgtt ttgacctatt acccttttgt gcaggtgctc ttccatggtc aaaaggcggc
     1501 tggtttcttc cacatcgaga gcggcaaccg gaatgcctgt catcatgctg accactgcgt
     1561 ggatcgcaat ctgcgatatc gtggccatga ttgccctgcc tcttgccacc gctagcggtg
     1621 acactgagac ggtggctatc ggtgtcgctg tggtgggcgt tgtggccatt gcttgcctcg
     1681 tgggcttgcg attcttccgc tcttctgata tcggtgagca cctgaggcag ctttcccgaa
     1741 gcaagcattg ggcactcgac cttcgtctct cccttgcgat gctcttcggg cttgcttggt
     1801 tg
//
LOCUS       DAOQNZ010000939         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47015_length_1801_cov_4.206186, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000939 DAOQNZ010000000
VERSION     DAOQNZ010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47015_length_1801_cov_4.206186"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1112)
                     /locus_tag="PKW73_15075"
     CDS             complement(144..1112)
                     /locus_tag="PKW73_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HNA74668.1"
                     /translation="MKIVIPGGSGELGTIIGKAFAENGYEVVILSRSNSSTTEKGITY
                     VQWDGISPGPWMQQIDGSDLVFNLAGRSVNCRYTDATRQEIMESRVLSTKAVGRAIEI
                     ANNPPPLWINMSTATIYAHSYDKGNDEFTGIIGGEEPSAPKSWGFSIKVAKAWEEAVD
                     NAAVPSLVRKVKLRSAVVMTPDPGGPFSIMSKLVRLGLGGKNASGKQMISWISDKDFI
                     KSIFFILNNPQLEDIVNVASPNPLANAVFMKVLREAWGKPIGLPAAEWMLSIGAVLLK
                     TETELVLKSRYVLPGKLLQAGFTFDQAQWPEAAADLVERTRSSKRH"
     gene            complement(1200..>1801)
                     /locus_tag="PKW73_15080"
     CDS             complement(1200..>1801)
                     /locus_tag="PKW73_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74669.1"
                     /translation="GDDVAFIDQVIDNLVSKKVADSSRIYSCGISNGGFFSQYLAQRL
                     KGKITAVASVAASVSEVMAASSMSREPIMFVLGDNDPLVPFKGGRVGGKLIRKSRGSV
                     IPFDSAIETYRSANSAGSLTLQKQLPDLAPADACRASVMRYGAEGDPAEVLVYKIDGG
                     GHTWPQGLQYLPAALVGPVCQDFNCNEAIWSFFSTHRKQ"
BASE COUNT          464 a          488 c          413 g          436 t
ORIGIN      
        1 aaaggaaaaa caacaaggac ttcccgatga tcttgtcggc agctttccaa aaaccacctg
       61 ctcgaaaatg tggtttaagg atttaaagac caactaattt ggaatctccc cgaaagcttc
      121 tgcaaaccac gccgcttaaa ggcttaatgc ctcttggaac ttcttgtgcg ttccaccaga
      181 tctgccgccg cctcaggcca ctgggcctgg tcaaaggtaa atccggcttg gagaagtttt
      241 ccaggtaaaa catagcgact cttgagcacc agctcggtct ctgttttgag caagacagcg
      301 ccgattgaga gcatccattc cgcagcaggc agtccgattg gtttacccca ggcttctctc
      361 aagaccttca taaagacagc attagcaagt ggattggggc tcgcgacatt gactatgtct
      421 tctagctgcg ggttattcaa aatgaagaaa atggacttga tgaagtcttt gtctgaaatc
      481 caggagatca tctgcttgcc ggaagcattc ttaccaccca agcccagacg aacaagcttg
      541 gacattatac taaacggtcc accgggatca ggggtcatca ctaccgcgct tctaagttta
      601 actttacgaa caaggctggg aaccgccgcg ttgtctacgg cttcctccca ggcttttgcc
      661 actttgatgg aaaagcccca ggacttaggt gcacttggct cctcaccacc gatgatacca
      721 gtgaattcat cattgccttt atcataagaa tgggcgtaga ttgtagccgt gctcatatta
      781 atccataaag gcggcggatt attagcaatc tcaatggccc gacctacagc cttggttgat
      841 aagaccctgg attccattat ctcctggcga gtggcatcag tataacgaca attgaccgag
      901 cgtccggcca ggttgaaaac caggtcgcta ccgtctatct gttgcatcca gggtccgggt
      961 gaaatgccgt cccactgaac ataagttatg cctttttctg ttgtggaact atttgagcga
     1021 ctgagaatca ctacctcgta accgttttcg gcaaaggctt tgccgataat ggtgccaagt
     1081 tcaccggaac cgcccggtat gactattttc actctaacac ttcgctccct cagtgcagaa
     1141 attttaccac ccggcggcaa ccgttttgag aaactgaata tcaagccacc ttaatagaat
     1201 cattgcttcc ggtgagtgct gaagaagctc catatagcct cattacaatt gaaatcctgg
     1261 caaaccggac caaccagagc ggccggcaaa tactgcaagc cctgaggcca ggtatggcca
     1321 ccgccatcaa ttttatatac caagacctcg gctggatctc cctccgcgcc ataacgcatg
     1381 acgctggccc ggcatgcatc ggcaggggca aggtcgggta gttgcttctg cagagtgagg
     1441 gaaccggcac tattggcgct gcgataagtt tctattgcac tatcgaatgg aatgaccgag
     1501 ccccggctct tccttatcag tttgccgccc actcgaccac ctttaaacgg aaccagcgga
     1561 tcattgtctc ccagcacaaa cattatgggt tcccgactca tggaacttgc tgccatgact
     1621 tccgaaacgg aggcagcgac gcttgccact gccgttatct tgcccttcaa cctttgggcg
     1681 agatactggc tgaaaaagcc accgttgcta atcccgcagg aataaatccg agaggaatcg
     1741 gcgacctttt ttgaaaccag attatcgata acctgatcaa taaaagcaac atcgtcgccc
     1801 t
//
LOCUS       DAOQNZ010000940         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47124_length_1799_cov_3.631881, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000940 DAOQNZ010000000
VERSION     DAOQNZ010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47124_length_1799_cov_3.631881"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1799)
                     /locus_tag="PKW73_15085"
     CDS             complement(<1..>1799)
                     /locus_tag="PKW73_15085"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA74670.1"
                     /translation="PQIFVKDIGKVEDSSDILAGYALFQGRRTVYIPAVKRADASTVS
                     VVDALNAALPRMQAVLPPDVKISYEFDQSKYVREAINDVFHEGVLGTILPGLMILLFL
                     RDLRSTLIVVTTIPLSLLSAIVGLWLCGQTFNIQTLSGLALSIGILVDEATVCIENMH
                     SHLAAGAVKSRAVFDACVETMVPRLLAMLSVVSVFLPSFFMVGITRELFIPLSLAVGF
                     AMIASTTLASTLVPIMAVWLLKDEHKHAGKPDFIDHLKDALGHFVRFLLPMRAVIIPV
                     YLAVTGLAVFSLYENIGKELFPDSGSSQFRMRISAPTGMRVEALEKRVLKAIEEIKLE
                     AGPENVEKTLGYAGQQPAMFPISSAFLWTSGPHQAVLDVQLKEDAHIKMGDFKDRLRT
                     RLNKALPDTTFSFEPGDIVSQIMNLGSPTPITVQVTGHDLVDDKLFANRVLEEMSKIS
                     KLRDLQWGQPLEYPSCQINIDRELAGQLGVTAKEVGMSLQPAFFSSRFVNLSLWRDKD
                     SGFSYQVQVQVPQDQIRSKEDIEKFPTMNSLRSEQMAGNDFEVDPAVDREYPESKTRR
                     LQHPLIGDVAKVDYGTVAGELDRYNMMRMVSLT"
BASE COUNT          460 a          473 c          504 g          362 t
ORIGIN      
        1 ggttagagag accatgcgca tcatgttgta gcggtccagt tccccggcca cggtgccgta
       61 atcaacttta gcaacgtcgc ctatgagggg atgctgcaaa cggcgagtct tgctttccgg
      121 gtactctcta tctacagccg ggtcgacttc aaagtcgttg ccggccatct gctccgaacg
      181 caaagaattc atggtgggaa acttctcaat gtcttccttg gagcgaatct gatcttgcgg
      241 cacctgtacc tgcacctggt aagaaaaacc gctatcttta tcgcgccaca agctcaagtt
      301 gacgaaacgg ctggagaaga aggcaggctg caacgacata ccgacttctt tggcggtgac
      361 accgagctga ccggccagtt ccctgtcgat attgatctga caggaaggat actcaagggg
      421 ctgcccccat tgcaaatcac gcagcttgct tatctttgac atctcttcca ggacacggtt
      481 ggcaaagagt ttgtcatcca ccagatcgtg gccggtgacc tgtacggtga taggggtggg
      541 agagccgagg ttcataatct ggctgacgat gtcgcccggt tcaaaagaaa atgtggtatc
      601 gggaagagcc ttattgagcc tggtgcgcaa gcggtcttta aaatcaccca tcttgatatg
      661 ggcatcttcc ttcaactgta catcaagcac agcctggtgc ggtccgctgg tccacaagaa
      721 ggctgagctg ataggaaaca tggccggctg ctggccggca taaccaagag tcttctctac
      781 attctcgggg ccggcctcca gcttgatctc ttcaatagcc ttgagcacac gcttttccag
      841 agcttccacc cgcatgccgg tgggagcaga aatacgcatg cggaactggc tgctgccgga
      901 atcggggaag agctctttgc cgatattctc atatagagaa aagaccgcca gcccggtcac
      961 cgccagatag acgggaatga tgacggcacg catgggcaga aggaagcgca caaagtgacc
     1021 gagggcgtct ttgagatgat cgatgaaatc aggcttgccc gcgtgtttgt gctcgtcctt
     1081 gagcaaccag acagccataa tcggcaccag ggtggaagcg agagtggtgg aggcaatcat
     1141 ggcaaaacca acggccaggg aaagcggaat aaagagttcc ctggttatgc ctaccataaa
     1201 gaaggaaggc aggaagaccg aaaccaccga gagcatggcc agaagcctgg gcaccatggt
     1261 ctctacgcag gcatcaaaaa cggcccggga tttaacggca ccggctgcca ggtggctgtg
     1321 catgttctcg atacagaccg tcgcctcgtc cacgagaata ccgatggaaa gagccagacc
     1381 actgagggtc tgaatattga aagtctgccc gcaaagccag agcccgacaa tggccgaaag
     1441 cagagagagg ggaatagtcg taactacaat cagggtactg cgcaaatcgc gcaggaagag
     1501 aagaatcatc agtccgggca aaatcgtacc caacacgcct tcatggaaga cgtcgttgat
     1561 agcctcgcgc acatacttgg actggtcgaa ttcatagctt attttcacgt cagggggcag
     1621 aaccgcctgc atacggggca aagcagcatt caaggcatcc accacggaaa ccgttgaggc
     1681 atcggcgcgt ttcaccgccg ggatataaac cgtgcgacgc ccctggaaga gggcataacc
     1741 ggccaggatg tcggaagagt cttccacctt gcctatgtct ttgacaaata tttgcggac
//
LOCUS       DAOQNZ010000941         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47189_length_1798_cov_2.733792, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000941 DAOQNZ010000000
VERSION     DAOQNZ010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47189_length_1798_cov_2.733792"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PKW73_15090"
     CDS             complement(<1..1442)
                     /locus_tag="PKW73_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74671.1"
                     /translation="MTAELAKPETKARIVRKDLQHTTLLVAATFAGSIVVALYVIAVC
                     ALNQQHKDSNSVDRAVYLAARSLSQITASHPSLGRVGLIDSAPPQEFSYHDRKKNPRV
                     IGLNTLYATLRVDAIIARKLNSPALIDLSARDFKIAKQLEAELTRRMFSAIEQVPEQD
                     PSLTEQPERVARQLTRAYTDGETEDDANQNESILQEVSRLLSEDVKGRGPNLVDVRLT
                     LGSVVAEKLGSQTYAPIEDSTKNFVKDGMYRAGIPVAMPDGQTITFLGQPRTTRIIDP
                     SWFKPRAQGTAPWAVRLDVIYERTKEKSAREKIVKTACAVVGGEPSYPRPSAFVLNFP
                     QGKPNQFGCLCDILNYKNWAGQGEWQQVVGTEVPGKGSLAPPIEAITGGLSPGSALAH
                     ALYDWMKQMGPLVEPDNFARMMNQSFAKAVKAASIDSATNQANSCLANDTGSREYALL
                     NQTNPDGPGQLALSHSFAIRDQRIAKNKIPP"
     gene            1492..>1798
                     /locus_tag="PKW73_15095"
     CDS             1492..>1798
                     /locus_tag="PKW73_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74672.1"
                     /translation="MLNLEPFLPINMVTRPEYAAAKFLPFGRNRYGAGSLVVLCLFIG
                     FSFGSQNRVESAAVAKSADGALSAELKTVNELTDARSFYKARKILEPLLAEHPKSSQV
                     "
BASE COUNT          375 a          458 c          471 g          494 t
ORIGIN      
        1 ggaggaattt tgtttttagc tatgcgctga tctcgaatgg cgaaagaatg gctcagtgcc
       61 aattgtccag gtccatccgg attggtctgg ttaagaaggg catattctct cgagcctgtg
      121 tcgttagcca ggcagctatt cgcctgatta gtggcgctgt cgattgatgc cgcttttacg
      181 gctttagcaa aagactgatt catcattctt gcaaagttgt cgggttcgac gagcggaccc
      241 atctgtttca tccagtcata tagagcgtgt gccagagcgc tgccggggga aagaccgcca
      301 gtgatagcct cgatcggggg agccaggctg cccttgccgg gcacttctgt gcccaccact
      361 tgttgccatt ccccctgtcc ggcccagttc ttatagttca aaatatcgca gaggcacccg
      421 aattgatttg gctttccttg aggaaagttg agtacaaatg ctgatggtct gggatatgat
      481 ggctcaccac ctaccactgc gcaggctgtt tttacaatct tttctcgcgc gctcttctct
      541 tttgttcgct cataaatgac atcaagtcgc accgcccacg gtgcagtacc ttgagcgcgc
      601 ggtttgaacc atgaggggtc tattatcctt gtcgtacgcg gttgaccgag gaatgtgatt
      661 gtctgcccgt ccggcatggc gacgggaatt cctgcacggt acatgccgtc tttcacgaag
      721 tttttggtgc tgtcctcgat aggagcatag gtttgagagc ccagcttttc tgctacaacc
      781 gagccaagcg tcagtcgaac atcgactaga tttggacccc tgcctttcac atcttcggat
      841 agcaatctgg agacttcttg cagaatcgat tcgttctgat ttgcgtcgtc ttcagtctcc
      901 ccatctgtgt atgcccgtgt taattgcctg gccactctct ccggctgttc tgtcagactg
      961 ggatcttgct cggggacttg ttctatggca gagaacattc gcctggtcag ttccgcttcc
     1021 agctgtttgg caattttgaa atctcttgcg ctcaaatcaa tcaaggcagg gctattcagc
     1081 tttctggcaa tgattgcatc tacccgcaga gtcgcgtaca gagtgttcaa gccaatcact
     1141 cgcggattct tttttctgtc atggtaggaa aattcctgag gtggtgccga atcaatcagt
     1201 cccaccctgc ccaaggatgg gtgcgaggcg gtaatttgag aaagcgagcg agcggcaagg
     1261 tagacggcgc ggtcgactga attagagtct ttgtgctgct gattgagcgc gcagacagca
     1321 attacgtaaa gagccacgac aatgcttccg gcaaaagtag cagcaaccag aagggtcgtg
     1381 tgctggaggt ccttgcgcac gattctggct ttagtctctg gttttgcgag ctcggctgtc
     1441 ataccttcct tcttccctat ttgataacat gcacactaga agtcgtgatt aatgctgaat
     1501 ctggagcctt ttctgccgat aaatatggtc actcgccctg aatatgccgc agcaaagttt
     1561 ttgccgttcg gtcgaaaccg ctatggcgcc ggttctctgg tggttctttg cctgtttatc
     1621 ggcttttctt tcggcagcca gaatcgagtc gaatcagcag cagtcgctaa aagtgccgat
     1681 ggcgcgctgt cggctgagtt gaaaactgtc aacgagctaa ccgacgctcg cagtttctac
     1741 aaagcaagaa aaatactcga gcccttgctg gcggagcatc ccaagtcttc acaggtgc
//
LOCUS       DAOQNZ010000942         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47272_length_1796_cov_4.854681, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000942 DAOQNZ010000000
VERSION     DAOQNZ010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47272_length_1796_cov_4.854681"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKW73_15100"
     CDS             complement(<1..602)
                     /locus_tag="PKW73_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74673.1"
                     /translation="MQGNSAWQVIEEDLIPFKVWSGFYTQEEILEEAVEALTEGWYEN
                     EEADEESIDCLSDEQIAKLKKIIARQFEEKRKAEKNWPESTDCDRLQNAFAELTERGI
                     VSLENAGYTLSDGWSDWDEVVNNEWHAKGLLNKVRGGCFYHGQDLERAVNGAGLNLAF
                     SAISGEDSDGLPIGREIMEVLKKHGFKPSWNGSITERIFMP"
     gene            661..>1796
                     /locus_tag="PKW73_15105"
     CDS             661..>1796
                     /locus_tag="PKW73_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74674.1"
                     /translation="MSPLTSPEQFAQMVAERLRKRIASAHVEYGASRFELFVKGDRGQ
                     LIGKVFLGNFYREFLESDLHGQELIMLRACQVIEAPDLAGIDFESRLYPQIKDRWSIE
                     KARLSSVHMPYVELSEHAALLLAYDLPERISYVGSEQLENWQLSFDMALHNASANLYS
                     RTTFEFEQVFDENTGALLFAVSPWRDHYDCGRFVFASEILKLPVQGEHVVFLISPSYM
                     FITGSESDRGLLFALSELRANKADPHALPPFPVLLNRDGYKPFEISPDNSLHHLFEEA
                     QAGYLKGVYADQRQCLFGQFNRLVPGLYICQYDVVPDGYRVGVRTSAVIDQRRLPCLV
                     PQVSQVHFRSGEGPASSASFAAFLSVMADAIQTTDLYPALYRLNR"
BASE COUNT          364 a          490 c          445 g          497 t
ORIGIN      
        1 ggcatgaaaa tgcgctctgt aatggaaccg ttccaggaag gcttgaagcc atgcttcttc
       61 aacacttcca tgatttcacg accgatcggc agtccgtcgg aatcctctcc ggaaatagcg
      121 gagaaggcaa gattcaagcc ggctccgttc actgcccttt ctaagtcctg gccatggtag
      181 aagcagccac ctctgacttt gttcaaaagc ccttttgcat gccattcatt gttcactacc
      241 tcgtcccaat cggaccagcc gtcacttaat gtatagccgg cattttccag agagacaatg
      301 ccccgctcgg tcagttcggc gaaggcgttt tgcagtctgt cgcaatcggt gctctcgggc
      361 cagttctttt ctgccttgcg tttctcttcg aactggcgag caatgatttt tttcaacttg
      421 gcgatttgtt catcactcaa gcaatctatc gattcttcgt ctgcctcttc attctcgtac
      481 cagccttccg tcaaagcttc cactgcttct tccaggatct cctcctgtgt gtaaaagccg
      541 ctccagacct tgaaaggaat caagtcttcc tcgatcactt gccaggcgct atttccttgc
      601 attttcagcc ctcgaatgaa ttggttcgaa cggtggtcct aaggatatat aatgatacca
      661 atgtctcccc ttaccagtcc cgaacaattt gcccagatgg tagccgagcg cctcaggaag
      721 cgcatcgctt ccgctcacgt ggagtacgga gccagccgct ttgagctctt tgtcaagggt
      781 gacagggggc agctcatagg caaggtcttt ctgggcaatt tctatcgtga gtttttggag
      841 agcgacctgc acgggcagga gctgataatg ttgcgggcct gtcaggtaat tgaggctccc
      901 gatctagccg gtatcgattt tgaaagtcgc ctctatccac agataaagga ccgctggtca
      961 atagagaagg caagactttc gtcggtgcat atgccctatg tagagctgag tgagcatgcg
     1021 gcgttgcttc ttgcttacga tttgcctgag cgtatttctt atgtgggtag cgaacaactg
     1081 gagaactggc agctcagttt cgatatggct ttgcataatg ccagcgccaa tctatacagc
     1141 cgcaccacat tcgagttcga acaagttttt gacgagaaca ccggtgctct gctctttgcc
     1201 gtttcgccct ggcgcgatca ctacgattgc ggccgctttg ttttcgcttc cgagatcttg
     1261 aaattgcctg tgcagggcga gcacgtggtc tttttgatca gccccagtta tatgtttatt
     1321 accggttccg aaagcgatcg aggtcttctg tttgctctat cggagctacg ggccaataag
     1381 gcagatcccc atgcgctgcc gccttttccc gtacttttga accgggacgg gtacaaacct
     1441 tttgaaattt cccccgataa ttcccttcac catcttttcg aggaagcgca ggccggctat
     1501 ctcaaaggtg tttatgcaga tcagcgtcag tgcctgtttg gacagttcaa ccggcttgtg
     1561 cccgggctct acatttgcca gtatgatgtg gtgcccgatg gctacagagt gggcgttaga
     1621 acttcagcgg ttatagacca gcggcgtttg ccctgtctgg tgccccaggt gagccaggtg
     1681 catttccgct cgggggaggg tccagcatcc tcagccagtt ttgctgcctt tctatcggtg
     1741 atggctgatg ccatccagac caccgatctc tatcctgccc tttaccgcct caatcg
//
LOCUS       DAOQNZ010000943         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47412_length_1794_cov_3.154112, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000943 DAOQNZ010000000
VERSION     DAOQNZ010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47412_length_1794_cov_3.154112"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..642)
                     /locus_tag="PKW73_15110"
     CDS             complement(163..642)
                     /locus_tag="PKW73_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74675.1"
                     /translation="MTTTAIKKTVVEATWNLAQVQAEAARTLGQQFMTIMSILPQYGE
                     KAVEDFRSAMRAQKIEHFKTLGVKTPFELVKATAEFETNVFGSKIEIWGDEKEAGMTY
                     LTCGMWEAMSKACTMTKEQEAAMGKSWEGCVTAMAAQFGFKAKLEMTEKTATITYTK"
     gene            complement(783..1754)
                     /locus_tag="PKW73_15115"
     CDS             complement(783..1754)
                     /locus_tag="PKW73_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74676.1"
                     /translation="MKTRKSLLKLTLFTTLAQMQTAALAQGWSIPAGQGPGQTARFEK
                     QTEEQLISQAKLETKRNNPEGALQICNIILKRNPKCTAAYVTKSKNLDFLNRDADALK
                     CLDEGLKILPQAPSLYREKGLILTAMNRNREALEAFNKAIENHCPEWDVYHQRSVVYG
                     SLKMHARAIDDMSTYIKLQPKRSRGFQWRAAAYAQSGQTDKAIQDLTTAIKMSPGKEG
                     EFLLERAELYMKVKDYQKAVADYAETLKTSPLDDTIWLKKGLALSKAGKPDQALKDFT
                     ECIELNGSSTAYLARADLLEKLGKHKEAAKDRARALQLEKEKAVEKL"
BASE COUNT          351 a          415 c          494 g          534 t
ORIGIN      
        1 gcagtcttgc tcggggtggg ttcctcggca gctgtcttgc cgagagggtg tgaaccggtc
       61 tttcaagtaa acaacgcaaa tgttgcaaag ttattgcagc gaaagcaaaa gagagagccc
      121 gggggctctc tctcttgaga tttcaggatt tgctctagag cattacttgg tgtaggtaat
      181 ggtagctgtt ttctcggtca tttccagttt ggctttgaag ccgaactgag cagccattgc
      241 ggtgacgcaa ccttcccagg atttgcccat ggcggcttct tgctctttgg tcatagtgca
      301 agctttgctc atggcttccc acataccgca ggtgaggtag gtcatgccgg cttctttttc
      361 gtcgccccag atttcgattt tgctgccgaa tacgttggtt tcgaattcag cggtggcttt
      421 gacgagttca aagggggttt tgacgccgag ggttttgaag tgctcgattt tctgagcgcg
      481 catagcggag cggaaatctt ctacagcttt ttcgccgtac tggggaagaa tgctcatgat
      541 ggtcatgaac tgttgtccaa gagtgcgtgc agcttcagct tgaacttggg cgagattcca
      601 ggtggcttct acaacggtct ttttgattgc tgtggttgtc atttttcgac tccgaggagt
      661 ggtgtgtgta acaggctcat aataatgtcc aatttgtaaa ctttagctcg atttaagcga
      721 ggcttagggt ttccctttga caaccgtcgc tcccatcctt tcatatagat gagatgcgcg
      781 aactaaagct tttccacggc cttttctttt tcaagttgaa gggctctggc tctgtcttta
      841 gcggcttctt tgtgtttgcc cagtttctcc agtaagtcgg ccctggccag gtaggcagta
      901 ctggaaccgt tcagctcgat gcattcggtg aagtctttca aggcctgatc aggttttccg
      961 gccttactga gggcgagccc tttcttgagc cagatggtat cgtccagggg gcttgtcttg
     1021 agtgtttctg cgtagtcagc cactgccttc tggtagtctt tcactttcat atagagttca
     1081 gctctctcca gaaggaattc accttccttg ccgggagaca tttttatggc agtggtcaaa
     1141 tcttgaatgg ctttatcggt ttgaccgctc tgggcatagg cggcggcacg ccactggaag
     1201 ccgcgagagc gcttgggctg taactttata taagtcgaca tgtcatcgat tgcgcgggca
     1261 tgcattttca gactgccata aaccacagag cgctggtgat aaacatccca ctccgggcag
     1321 tgattctcga tggccttgtt gaaggcttcc agggcttccc ggttgcgatt catagctgtg
     1381 agaatcagcc ccttctctcg ataaagactg ggggcttgag gcaaaatctt caagccctcg
     1441 tcaagacatt taagagcgtc ggcatctcga ttgaggaagt ccaggttttt ggattttgtg
     1501 acataagcgg cggtgcactt agggttgcgt ttaaggatga tgttgcagat ctgcaaggca
     1561 ccttcgggat tgttccgctt cgtctccagc ttagcctgac tgataagttg ttcttcggtt
     1621 tgtttctcga agcgggcggt ctgcccgggg ccttgccctg ccggtatgga ccaaccctga
     1681 gccagggcag cggtttgcat ttgtgccagg gtcgtgaaga gcgtcaattt aagcagtgat
     1741 tttcttgtct tcattcagcg aggacctgcc tggcttagaa aaattatgat tagc
//
LOCUS       DAOQNZ010000944         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47447_length_1793_cov_4.976410, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000944 DAOQNZ010000000
VERSION     DAOQNZ010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47447_length_1793_cov_4.976410"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1793)
                     /locus_tag="PKW73_15120"
     CDS             complement(<1..>1793)
                     /locus_tag="PKW73_15120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA74677.1"
                     /translation="SAVRRAVAPFTQERVFLCDGEGIPKFLDSHFPAEALALRAPDWE
                     LVSEKGWMAVCEDSSQAVSGENSWRTNCGKGRPFQLELSLKCLDGQNRWYRIYAAPFE
                     EAGAAYQWCGTCRLVDDERKLLVALEHARRVKSEFVANVSHELRTPLNGILGMIELLL
                     RGPLSEEASEHVLMMQEAGRSLLSIVNEVLDFSKIEAGKLVVEKRDCDIVSVVEAVTQ
                     ILTPAAAAKNILLVSSVSQLESQSFRTDPQRLRQILLNLAGNAVKFTLSGHIVLKVES
                     CRLKDSDLDWLRFSVCDTGIGINPDQIEKLFEPFAQSAAIGVRDKSGTGLGLTISKRL
                     AELLGGVIEVQSVPDLGSTFSLLLPLEDELAETALSDIWSKPSRPVSRPVRLLCLEPI
                     VNNVAVLSDVFESFDLLAQSASSAEAAIRIINQAEQDGRPFHAVVLDFVRNEKECQSM
                     LQYLQYTGLSKVLKTVAVYGCEADAASTECTYSLFMPLLRSHILELISHIAPDFGKGE
                     VLSRAAQNPRLTSRIAVSEPGAVGAKVPVVSVGDRRALVADDHAINQKVLNLFLSDLG
                     FEVDVTVDGVEAVHAFKEKGYSIVFLDCQMP"
BASE COUNT          471 a          515 c          439 g          368 t
ORIGIN      
        1 ggggcatctg acaatccaga aaaacaatac tgtagccttt ttctttgaaa gcatggacag
       61 cttccacacc atcgacggtg acgtctactt caaaacccag atctgagaga aagagattca
      121 gcaccttctg attgatggca tgatcatccg ccactagcgc ccgccggtcg ccgacagaaa
      181 caaccggcac tttagcgccg acagcgcctg gctcgctcac ggcaatacgg ctggtgaggc
      241 gggggttctg ggcggccctg ctgagcacct cgcccttacc aaagtcgggt gcgatatgag
      301 aaatcaattc caaaatatga ctgcgcagaa gtggcataaa taaactgtaa gtgcactcgg
      361 tagatgcagc atcagcttcg cagccgtata cggcaaccgt cttgagaact ttactcaaac
      421 cggtgtactg caaatattgc agcatcgact ggcattcctt ttcattacgc acaaagtcca
      481 gtaccacggc atgaaatggt cgcccatcct gttccgcctg gttgattatg cgaatggccg
      541 cttccgctga acttgcagac tgagcaagca aatcaaagct ctcaaagaca tcggacaaga
      601 ctgccacatt attgactatc ggctccaggc ataacagtcg gacaggtctg gaaactggac
      661 ggctcggctt actccagata tcactgagag cggtctcggc cagttcatct tccaggggca
      721 gaagcaaaga aaacgtggag cctagatcag gcacactctg cacctcaatg acaccgccta
      781 gaagttcagc cagtcgcttg gaaatcgtca ggccaagacc ggtgccggac ttatcgcgca
      841 ccccgatagc agcggactgt gcaaaaggct cgaataactt ctcaatctga tccggattga
      901 taccgatacc ggtatcgcag acagaaaatc tcaaccagtc cagatcacta tctttcaggc
      961 gacatgactc taccttcaag acaatgtgac cggaaagcgt gaacttgacg gcgttccccg
     1021 caagattgag caaaatctgg cgcaagcgct gtgggtcagt acgaaaactc tgggactcga
     1081 gctgagacac cgatgagaca agcagtatat tcttagccgc agcggccgga gtcaatatct
     1141 gcgttaccgc ctccacaacg gagacgatgt cgcaatcgcg tttttccacc accaacttgc
     1201 cggcctcaat ttttgagaaa tcgagaacct cattgacgat actcaataga gaccgaccgg
     1261 cctcttgcat catcaaaaca tgctcgctgg cctcttcact gagaggaccg cgcagaagca
     1321 actcaatcat tccgagaatg ccgttaagcg gagttcgcag ttcatgagaa acattggcta
     1381 caaattccga cttgaccctg cgggcatgtt ccagagcaac aagaagtttc cgttcatcat
     1441 caactaagcg gcaggtgccg caccattgat aggccgcgcc cgcctcctca aagggggcgg
     1501 cgtaaattcg ataccagcgg ttctgcccgt caaggcactt gagggaaagc tccaactgaa
     1561 acggtctgcc tttgccgcaa ttagttcgcc agctgttctc acccgacact gcctggctgg
     1621 agtcttcaca gactgccatc cagcctttct ccgatacaag ctcccaatcg ggtgcacgca
     1681 gggctagtgc ttcggcagga aagtggctat ccaggaattt gggtataccc tccccatcgc
     1741 acagaaagac gcgctcctga gtgaaaggag ccaccgcccg acgcaccgcg gac
//
LOCUS       DAOQNZ010000945         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47558_length_1791_cov_3.682604, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000945 DAOQNZ010000000
VERSION     DAOQNZ010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47558_length_1791_cov_3.682604"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..>1004
                     /locus_tag="PKW73_15125"
     CDS             447..>1004
                     /locus_tag="PKW73_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74678.1"
                     /translation="MAGSNTPENQIKKAPDAVKEGGEAEKARLEASQCMADEAYKPSE
                     NPEDFNRMLRGIEDFRSSSRAILALNDNEQFNSGSKIFSDGSKFKGGIAGDSHLGNRQ
                     LTATGAEQKPAAVDTKTAKVSMDSILPKLTLQDGSAKAPAERPIGKSTPVAGNFDFLG
                     DVFTNGAPGEFNATKNYEQNSNQVMA"
     assembly_gap    1005..1014
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1015..>1791
                     /locus_tag="PKW73_15130"
     CDS             <1015..>1791
                     /locus_tag="PKW73_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74679.1"
                     /translation="ADKPLTLNTAEEAKARAEQISKFRDDSETTFNQFAEIEKNAKPG
                     EKLEKEGDAIVKRNERGEEIFRREGSVVTITKDGRSYVMDEKTGTREVWSADGKLELR
                     RYSSGLLETSDGGDGLIRVNPNTGATWMTDKNGNIISDIRNTTDGGKILEVQNTKILT
                     VNETIDTSDAAALAALINQKEKEGFSGVIHFKNGSLLVNMSAEVKDANGKVVPGNQNR
                     KSAFISHDGTVMQEVDGAFVFRRNAKDAKGNLIGEAGIVD"
BASE COUNT          533 a          508 c          431 g          309 t
ORIGIN      
        1 aggtggtcag cttcatcttg ccgccctgat tctggcgcac gaaacagaag ccccggtcgc
       61 cgtccttgag ggcacaggca cgcgggcaca ggtcgcagac aagcgcgagg ccgtcagccg
      121 agacatggct gtagcctaca tctattgtga aatcttctac ttcccgttta accatacttt
      181 ccgtttgctc accgatgaca aacttccgca cgctctccca taaaaccggc cgccgctagt
      241 aagaggacgg ttcagcagcc cccgctacct taagtatagt gggctccagc ctctaaagtt
      301 gctccccgca gagcagaaac tccgccgcca aaaactctcg gcattaaaga ttaaatcccc
      361 cggcagcctt tccaaaattt ccacccgccg catatatccc tatgtgcagg cgggacattt
      421 cttaaaacgg ttaatcacag gttactatgg caggctccaa caccccggaa aaccaaataa
      481 agaaggctcc ggacgctgtc aaagaaggcg gcgaagccga gaaagcacgc ctggaagctt
      541 cccagtgcat ggccgatgaa gcctacaaac cttcagaaaa ccccgaagat ttcaacagaa
      601 tgctcagagg cattgaagac tttcgttctt cctccagagc catcctggct ctgaacgaca
      661 acgaacagtt caacagcggc agcaaaatct tctccgacgg cagcaagttc aaaggcggca
      721 ttgccggcga cagccacctg ggcaaccgcc aattgaccgc cacaggcgcc gagcagaagc
      781 ccgctgccgt cgacaccaag actgcaaaag ttagcatgga cagcattctg cccaagctca
      841 cccttcaaga tggcagcgcc aaagcaccgg ctgagcgccc catcggcaaa tcgacaccag
      901 tagctggaaa ctttgatttc cttggtgacg tattcaccaa tggcgctccc ggagagttta
      961 atgccactaa aaactatgag cagaatagca atcaggtgat ggctnnnnnn nnnnccgccg
     1021 ataaacctct gacactgaac accgccgaag aagccaaagc cagagctgaa caaattagca
     1081 aattcagaga cgacagcgaa actaccttca accagtttgc tgaaatcgaa aaaaatgcaa
     1141 aaccaggcga gaagctggaa aaagagggcg atgccatcgt taagcgtaac gagcgcggcg
     1201 aggaaatctt cagaagagaa ggcagcgtag ttaccatcac caaagacggc agaagctatg
     1261 taatggacga aaagaccggc acccgggaag tctggagcgc cgacggcaaa ttggaattga
     1321 gaagatacag cagcggtttg cttgagacct ccgacggcgg cgacggcttg atcagagtca
     1381 acccgaacac cggcgccacc tggatgaccg acaaaaacgg caatatcatc tccgatattc
     1441 gcaacacaac cgacggcggc aaaattctag aagtccaaaa caccaaaata ctgaccgtca
     1501 atgaaaccat cgacaccagc gatgctgccg ctctggcagc cttgatcaac cagaaagaga
     1561 aagaaggctt tagcggcgtc attcatttca aaaacggcag cttgctcgtc aacatgagcg
     1621 ccgaagtgaa ggatgccaac ggcaaagtag tacccggaaa ccagaacaga aaatccgcct
     1681 ttatttccca tgacggcact gtcatgcaag aagtagatgg agccttcgtc ttcagaagaa
     1741 acgccaaaga tgcaaaaggc aatctcatcg gcgaagctgg tatcgttgac g
//
LOCUS       DAOQNZ010000946         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47570_length_1791_cov_3.040899, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000946 DAOQNZ010000000
VERSION     DAOQNZ010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47570_length_1791_cov_3.040899"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PKW73_15135"
     CDS             <1..793
                     /locus_tag="PKW73_15135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA74680.1"
                     /translation="PANYYSYNYDSAISPLAVKAKDFLDKRKTRGWLKHVSTKELRFL
                     DVGCGNGRNLQILAELGYARQNLYGMEINGDTVERIRKQGFNGVQGRVEEAAKDLEAA
                     SFDLIVLLQVLEHVGSPQAVIESLAKLLKPGGVLVVETPNSKSLDVDLFRKRYWGGYH
                     FPRHWYIFNPDNLEKLAAAAGLTVKEVNYLPAQSFWIYSLHHIVKEKIAIPWVVRLFN
                     PFQNLLLLSLFTSFDIVRAKLGAKTSNMQMILLRKQELEQDDKPR"
     gene            790..1704
                     /locus_tag="PKW73_15140"
     CDS             790..1704
                     /locus_tag="PKW73_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA74681.1"
                     /translation="MTSQDDQPDKLSPLVSVVMPCLNEEKALGICIEKIQKTFKEHNI
                     DGEVVVSDNGSTDRSVEIAQSMGANLCHQPLKGYGNAYLKGFEHARGRYLIMADADDT
                     YDLTMIPDFLKGLQEGKYDFVTGSRYLGKGEKNITWLHRYVGNPMLTFILNLLFATRY
                     SDVYCGYRAFTREAYEKIPAGQPGHGIQPGTGYKRSTCRFAHPGNTDHTGGAPGRVEA
                     QHLQRRLAQPAHDAYLRPQQGFSHSRAVPLWRRHAFAPGRALTAHSVGRKGTGRSISD
                     GGIDHGRNRLPDLEPLAPCQNLFLEPAL"
BASE COUNT          464 a          476 c          446 g          405 t
ORIGIN      
        1 cccggccaac tattattctt acaattacga cagcgccata agtcccctgg cggtgaaagc
       61 caaagacttc ctcgacaaga gaaaaaccag aggctggctc aaacatgtct ccacaaagga
      121 attgcgcttt cttgatgtgg gctgcggcaa cggcagaaac ctgcaaatac tcgcagaact
      181 gggttatgcc cgccagaacc tctacggtat ggaaatcaac ggcgataccg tagaaagaat
      241 tcgcaagcag ggtttcaacg gtgtgcaggg gcgagtggaa gaagcggcaa aagaccttga
      301 ggcagccagt ttcgacctga tagtgctgct tcaagtgctg gagcatgtag gaagtccgca
      361 agcggtgata gagtccctgg ccaaactgct caagccgggc ggcgtactgg tagtggagac
      421 acccaacagc aagagtctgg acgtagactt gtttcgcaaa cgttattggg gaggctatca
      481 ctttcccagg cactggtaca tcttcaatcc cgacaacctg gaaaaacttg ctgcggctgc
      541 cggtttgaca gtcaaagagg tcaattacct gccggctcag agtttctgga tttactcgtt
      601 gcaccacata gtgaaagaga aaatagccat cccctgggtt gtcaggctct tcaacccctt
      661 ccagaatttg cttctattga gtctcttcac cagtttcgat atagtgaggg caaaattggg
      721 agcaaaaacc tccaacatgc agatgatttt gctgcgcaaa caagaacttg aacaagacga
      781 caagccaaga tgacaagcca agatgaccag ccagacaaac taagccccct ggtatcagtg
      841 gtgatgccct gcctgaatga agaaaaggca ctcggtattt gcattgaaaa aatccagaaa
      901 acttttaagg agcacaacat cgacggcgaa gtggtggtct ccgataacgg ctccaccgat
      961 cgctccgtgg aaatcgccca gtcaatggga gccaacctct gtcaccagcc cctcaaaggt
     1021 tacggcaatg cttatttgaa aggctttgag catgcccgcg gccgttatct gatcatggct
     1081 gatgccgacg acacttatga cctgactatg attcccgact tcctcaaagg tctacaagag
     1141 ggcaaatatg actttgtcac gggcagccgt tatctgggca agggcgaaaa gaacatcacc
     1201 tggctgcacc gctatgtggg caatcccatg ctcaccttca tcttgaacct cctctttgcc
     1261 acccgttatt ctgatgtcta ctgcggttac cgcgccttca cccgagaagc ttacgaaaaa
     1321 attccggccg gtcagcccgg gcatggaatt caacctggaa ctggctataa acgctcaact
     1381 tgccggtttg cgcatccagg aaataccgat cacactgggg gagcgcctgg gcgagtcgaa
     1441 gctcaacacc ttcaaagacg gctggcgcag cctgcgcatg atgcttattt acgcccccaa
     1501 caaggttttt ctcattcccg ggctgttcct ctttggcggc ggcatgcttt tgcacctggt
     1561 cgtgctctta cggctcattc agtcggaagg aagggcactg ggcgtagtat cagcgatggt
     1621 ggcattgacc atggccgtaa ccggcttcca gatcttgagc ctctggctcc atgccaaaac
     1681 ctattcctgg agccggcgct ttgacagaaa caatcctttc atactttcct tctacaaatt
     1741 gtttaagttg gaaacaggcc tgattacagg actattgtta accttgaccg g
//
LOCUS       DAOQNZ010000947         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47638_length_1790_cov_2.532565, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000947 DAOQNZ010000000
VERSION     DAOQNZ010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47638_length_1790_cov_2.532565"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PKW73_15145"
     CDS             complement(<1..575)
                     /locus_tag="PKW73_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74682.1"
                     /translation="MSELPAGDYFPLGAGLEDSDRLYLVSTLKRLQERPLAEVGPKAI
                     AYRFIWLRSFHQPVSVMVYLPDSGVGLLVGKVIELEPRRSPSEPLRHDQLRTVVVNLP
                     TERCSQFKASLARFAFSQIDQYDDLTRPPLYDFELFGKRFSSRTEYMKDGARWVMEGW
                     KHGSYRVCQRQSPPDDDALRQMAIFMLTEAKL"
     gene            complement(705..>1790)
                     /locus_tag="PKW73_15150"
     CDS             complement(705..>1790)
                     /locus_tag="PKW73_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNA74683.1"
                     /translation="DKDYWKHIVSANNSFGVELLKTIALAKPLDNIFISPTSIFLLMS
                     MLYNGAAGDTYLQMAAGLKLPNPQEINPWNRQLINHLTSANFSHELLLASALWFGKGI
                     LCHDQFVNSCIESYSASLYGIDFESPEAPSQINFWAANATRGNIDQVVGENILGASLM
                     VTNAIYFFARWTDQFDRSQSAAGPFHMAGGGTREAIFMSKTAQYEYFESEAFQVLKLP
                     YANSPFFMQVILPKPGFHFGHCLPALAQPTMPMIPCELQLQMPRFTLEFEFEVKEFLP
                     RLGIDNFNDFSLICPGLQVSNVLHKSKLKVDEEGTEAAAVTAAVGWGSAPRQALLMRV
                     DRPFLLNIVDGGSGQILFSGAVVAPQS"
BASE COUNT          491 a          465 c          458 g          376 t
ORIGIN      
        1 agtttggctt cagtcaacat aaagatagcc atttgcctga gcgcatcgtc atctggtggg
       61 ctctgcctct gacaaactcg ataggagccg tgcttccaac cttccatgac ccagcgtgca
      121 ccatctttca tatactcagt gcggcttgag aagcgcttgc cgaaaagttc aaagtcgtaa
      181 agcggcggac gggtcaggtc gtcgtactga tctatctgag agaatgcaaa ccgggcaagg
      241 ctcgccttaa actgcgaaca acgctcggta ggcaagttaa caaccacggt tctcaattgg
      301 tcgtgccgca aaggttcgga ggggctgcgc cggggctcca gctcaattac ctttcccacc
      361 aacaacccga caccactatc gggaaggtat accatcacgc taacaggttg atggaaagag
      421 cgaagccaga taaagcggta ggcgattgcc ttcggaccga cttccgctaa cggacgctcc
      481 tgcaacctct tgagtgtgga aaccaaatac aatctatccg aatcttccaa accggcgccg
      541 agggggaaat agtcaccagc aggaagctca gacaaagaga gctttctggt tgaaagcaca
      601 gccggactgc aaccggttag gaacagagca gcaaccatga ccagacacaa gactcgagcc
      661 atagtcacct tacttttaca acccaaaccg cctgtcaggc tattttaact ctggggtgct
      721 accacggcac cagaaaagag aatctgccca ctgccaccat ccacgatatt aagcaggaag
      781 gggcgatcga ctcgcatcag aagggcttgc cggggagcac taccccagcc gactgctgcc
      841 gttactgccg cagcctccgt gccttcttcg tccactttca gcttcgattt atgtagcaca
      901 tttgaaactt gcaaacccgg acaaatcagc gaaaaatcat tgaagttatc gattcccaac
      961 cgcggcagaa actctttcac ttcaaactca aactcaagag taaagcgagg catttgcaat
     1021 tgcagctcgc agggaatcat gggcatggta ggctgagcca gagccggtaa gcaatgtccg
     1081 aagtgaaaac cgggcttggg caaaatcacc tgcatgaaaa acggcgagtt ggcatagggc
     1141 aacttcaaga cctgaaaggc ttccgactca aagtactcat actgagcggt cttagacata
     1201 aaaatggctt ctctggtgcc gccgccggcc atatgaaaag gcccggcagc actctggctg
     1261 cgatcaaact gatcggtcca gcgggcaaag aagtaaatgg cattggtgac cataagagag
     1321 gcgccaagga tgttctcacc cactacctgg tcgatattac ccctggtggc attggccgcc
     1381 cagaaattta tttgacttgg agcttccggt gactcgaaat caatgccgta gagagaagcc
     1441 gaataggact caatacaact attgacgaac tggtcgtggc acaaaatgcc cttaccaaac
     1501 cagagggcag aggccagcaa aagctcatga gagaaattag cggatgtgag gtggtttata
     1561 agttgccggt tccagggatt tatctcctgg gggttaggca gtttcaaacc ggcagccatc
     1621 tgcaagtagg tatcgccggc tgcaccgtta tagagcatcg acattaacag aaagatgctg
     1681 gtaggcgata tgaatatatt gtccagcggc ttggccagag caatggtctt gaggagttcc
     1741 accccgaatg aattgttcgc ggagactatg tgtttccagt agtctttgtc
//
LOCUS       DAOQNZ010000948         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47667_length_1789_cov_3.622261, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000948 DAOQNZ010000000
VERSION     DAOQNZ010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47667_length_1789_cov_3.622261"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKW73_15155"
     CDS             complement(<1..456)
                     /locus_tag="PKW73_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74684.1"
                     /translation="MFRQAFSEEGGLLQVVFQLSLALTLLAAPAAFSQGYAPSYKTVR
                     SAGIKVLSIDERAAERTIYVKLARDAVVGRPLFNYLPGEGGDTILTADFEGLSSNLPS
                     RVIRLNSPENRLNREARLSGIKEIHFAEIQADKPRLRFSFVARDPQILKS"
     gene            802..>1789
                     /locus_tag="PKW73_15160"
     CDS             802..>1789
                     /locus_tag="PKW73_15160"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNA74685.1"
                     /translation="MMGDTTLFGVLGCGAVALAYGGWAGSSVLSLSPGNERMQQIAAA
                     IQEGASAYMNRQYTTVAGVGAVLFIIIGLTLNWVTAAGFLVGAVLSALSGFVGMYVSV
                     RANVRTAEAARNGLEAALSVAFKGGSVTGLLVVGLGLLGVAGFYMVTNDVNALVGLGF
                     GASLISVFARLGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCAG
                     MAADLFETYAVTAVATMLLAHQVFHAQIEAGGAQNVIVYPLVLGAASIVTSVIGAMCV
                     KLGKSTNIMDALYKGLIVAGVLAGVSFWFITDKMMQGLQIGGKDVTTMSFFLA"
BASE COUNT          403 a          491 c          483 g          412 t
ORIGIN      
        1 actcttcaat atctgcgggt ctctggccac aaaggaaaat cgcaaacgcg gtttatcggc
       61 ctgaatctcg gcaaaatgaa tttccttaat gcccgataag cgagcttccc gattgagacg
      121 attttcaggt gaattcagtc taattaccct tgagggcaag ttgcttgaca gcccttcaaa
      181 atcggcagtc aagatggtgt cgccgccctc accgggcagg tagttgaaaa gtggacgccc
      241 gaccacggca tcccgggcca gcttcacata tatagtgcgc tcagccgccc tctcatcgat
      301 ggagagaacc ttgataccgg cgctcctgac agttttatag gagggagcat agccctgaga
      361 gaaagcggca ggggcagcca gcaaggttag ggccaggctg agctgaaaaa ctacttgcag
      421 gagcccgcct tcttcactaa atgcttgccg aaacacccgc atacttgact ttagaacgcc
      481 ttcttgacga caacctgttt tgaccttcgg ttattggccg gggccaataa aaccagatcc
      541 aaagaggttc atcataacat ggactacttt tatacaacct gcataagcaa tcgataactg
      601 ctaaccatta gcggtatcaa gacgcggtat caagcaaaat taaattgagt tccggctaaa
      661 gggctcacca cctggtaatc tggcagagca cttagctgct tagttatcag ttaggggcaa
      721 aacgaattat cagaaaaact gaaatattcg aacgcccggg gcaatcctgg atgcagttcg
      781 ttaagcgaat ttagggagta aatgatgggt gatacgactc tatttggtgt gctgggctgc
      841 ggcgcagtag cactagcata cggaggctgg gccggctcca gcgttctcag tctcagtccc
      901 ggcaatgagc ggatgcagca gattgccgcc gccattcagg aaggcgccag cgcctacatg
      961 aacaggcagt acaccacggt agccggtgtc ggtgccgtgc tcttcatcat tatcggcttg
     1021 accctcaact gggtcacagc cgccggcttc ctggtgggcg ccgtgctctc cgctctctcc
     1081 ggtttcgtcg gcatgtacgt ctcggtgaga gccaatgtgc gcacggcgga agccgcccgc
     1141 aacggtctgg aagcagcgct ctccgtagcc ttcaaaggtg gctcggtaac cggtctcctg
     1201 gttgtcggtc tcggtcttct aggcgtggcc ggcttctaca tggtcaccaa tgacgtgaac
     1261 gccctggtcg gtctcggctt cggcgccagt ttgatctcag tcttcgcccg tctgggcggc
     1321 ggtatctaca ccaaggccgc cgacgtcggc gccgacctcg taggtaaagt agaagccgga
     1381 attcctgaag atgatccgag aaaccctgcc gtaatcgccg acaacgtcgg cgacaacgtg
     1441 ggtgactgcg ccggtatggc tgccgacctg ttcgaaacct atgccgtcac cgccgttgcc
     1501 accatgcttc tggctcacca ggtattccat gctcaaatcg aagccggtgg tgctcaaaac
     1561 gtcatcgttt atccgctggt tctaggtgcc gcttccatcg tcacctcggt cataggggcc
     1621 atgtgcgtca agctcggcaa gagcaccaat atcatggacg ctctctataa aggtctgatc
     1681 gtagccggag ttctggccgg tgtttctttc tggttcatca ccgataagat gatgcagggt
     1741 ctgcagattg gcggtaaaga cgtaactaca atgtctttct tcctggctt
//
LOCUS       DAOQNZ010000949         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47668_length_1789_cov_3.618224, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000949 DAOQNZ010000000
VERSION     DAOQNZ010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47668_length_1789_cov_3.618224"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PKW73_15165"
     CDS             <1..1329
                     /locus_tag="PKW73_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74686.1"
                     /translation="RVELRADGSEYSLDLKEDDDAPGARLSEEDAKALALAYIRRVHP
                     EYKDFKLSRLSREDEKNRRDYSLDFVVPSLKVGEAEYKFYLQVVGDLPCNFAQAWTIP
                     DSWRYERIKETKQDKIFATVRLYTKVIIVLVLLYWVFCLLRAGVIRWRLPLVVAAVLA
                     LVPVLEQINHWPSFMAQYETHLTEATYVFNRFLQLVQHSASVFWQLFLALALSLGAFR
                     ILSPRSTFAAILTTAFRPRALQDRLSQKEMWLDGIIVALCGEALSQLKETLTYFLYYF
                     YSPEIRGAQIDVLCSLSDYFSAVFSDLLDLPQSFLLAIAIYLIGAGMYMKFCRNFWFY
                     MGFCLLYDLIMCSGERHWQDYLIALLSGLINSVIVWFFIAKLAKLNPVAYLLKIVLDD
                     CLPFIYAIYAYGLPAFAPDMVAFLIYLLAPMAVLLYLSLRNRSLSREATQ"
BASE COUNT          344 a          422 c          483 g          540 t
ORIGIN      
        1 agggtcgagc tgagagctga cggttccgag tattccctgg atttgaaaga agatgacgat
       61 gcccccggcg ctcgactctc tgaggaggac gccaaggcgc tggctctcgc ttatatcagg
      121 cgcgtgcacc ccgagtacaa agactttaaa ctctccaggc tttcgaggga ggatgagaag
      181 aatcggcgtg attacagcct ggactttgtt gtgccgtctt tgaaggtcgg ggaagccgag
      241 tataagttct atttgcaggt cgtgggcgat ttgccctgta attttgccca ggcctggaca
      301 atacccgata gttggcgcta cgagagaatt aaagagacca agcaagacaa gatatttgct
      361 acggtgcgcc tctacactaa ggtgatcata gttttggtct tgctttactg ggtgttctgc
      421 ctgctgcgcg ccggtgtaat ccgctggcgg ctgcccctgg tggtggcggc tgttctggct
      481 ctggtgcctg ttcttgaaca gatcaatcac tggccttcat tcatggccca gtacgaaact
      541 catttgaccg aagctaccta cgtattcaat cgttttttac agcttgtgca gcatagcgcc
      601 agtgtcttct ggcagctatt tctggcgctg gccctttctc ttggggcttt tagaattctt
      661 tcgccccgct ctactttcgc tgccattttg accactgctt ttcgtccgag agcattgcaa
      721 gaccggctct cgcaaaagga aatgtggttg gacggaataa tcgttgcact ttgcggggag
      781 gcgctcagcc agctgaagga gaccctcacc tattttctct attatttcta ttcgcccgag
      841 attcgcgggg cgcaaataga tgttttatgc agtctcagtg attatttctc ggcggtcttt
      901 tccgatctgc tcgacttgcc tcagtctttc ttactggcca ttgccattta tttgataggc
      961 gccggcatgt atatgaagtt ctgccgcaac ttctggttct acatgggttt ttgccttctc
     1021 tacgatttga tcatgtgcag cggcgagagg cattggcagg actacttaat tgctcttcta
     1081 tccggtctga tcaatagtgt gattgtctgg ttcttcattg ctaaactggc aaaactgaat
     1141 cctgtagcct atttgcttaa aattgtgctg gatgactgct tgcctttcat ttatgccatt
     1201 tatgcctatg ggctgcctgc ctttgctccg gatatggtgg cattcttgat ttacctgctt
     1261 gcgcctatgg ccgtgcttct ttatctgagc ctgcgcaatc gcagcctctc gcgggaagcc
     1321 acgcagtaga tactgcagtc gcttgggggc tcctcccccg accgggggaa ggctagttca
     1381 cgaatttaga gtaattttac agaagggttt ccttttgcaa atagttttat gcctgatggc
     1441 gcttcggcgg ttgacaagct ggatggctgg gaatacgctc aatataggta ggctattaat
     1501 tagctctggt aattggcttt ggcggtttga attcgattgg tgcggcggca tttttttgtc
     1561 gttctgtttg acgtgtttat aagttgctgc atcaggccaa atggttgttt tggcgcttgc
     1621 agcttttcgc gggtttagct attggacggc attgaagagt cggacaactg catagaaaaa
     1681 cggtccgagg acactactca cctggagtcc gcggtgcgtc gggcggtggc tcctttcact
     1741 caggagcgcg tctttctgtg tgatggggag ggtataccca aattcctgg
//
LOCUS       DAOQNZ010000950         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47685_length_1789_cov_2.960784, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000950 DAOQNZ010000000
VERSION     DAOQNZ010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47685_length_1789_cov_2.960784"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKW73_15170"
     CDS             complement(<1..682)
                     /locus_tag="PKW73_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74687.1"
                     /translation="MPELYVQADLELKSFRGEGDLPANHERLNSLDVLAKGGMELTGN
                     SSPKEGFLKLAPPPDWVRAPLSYSGEPRSSLEAYCAPANADVTLNFYDRGHDLSPAGR
                     KSFQEILERKGSSEKAQPLLPSEVAAMSEVFGINSVGDNQFSNGNMAGGAAGPVFHLD
                     MAYAQMVQGHRSVFVEGRFVDENGAPQREFVGAYIEGAKGAGSRVQEVYLEGSYSDRA
                     KPDGFLSHR"
     gene            complement(695..>1789)
                     /locus_tag="PKW73_15175"
     CDS             complement(695..>1789)
                     /locus_tag="PKW73_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74688.1"
                     /translation="PEPAPEPKPEPKPEPAPEPLEPPRPEPEEPPKPGEGSVEQARQE
                     LERSSAQIEDPAARQRFVQDCADFEKRAKEEGLPVEEVVKTMEATTRLLNSETGAVPE
                     AERINAAQGIMRHAAHPESIDQGAHGTCNVATIENMAFTDRPGEAASMISEVALTGKW
                     TAPDGKQIKIDAQSLIPGAEERTFPPQDGARSFASQLYQVTALNDLGQRENPPLCFSQ
                     RRPASRSDTGEYWTDSSGRILEPFGGLTVGEIADEAERLLPGDNTVVMLDANANWQDG
                     SIIAFNNEKDLTNKLLELKENNDLPIILAVNGSDPVFGGGSPNNGINHVICISDIDTS
                     TNPPRVQIDNQWGSRNDGWMSVSDLYNGCS"
BASE COUNT          405 a          539 c          421 g          424 t
ORIGIN      
        1 aacgatgaga gagaaatcca tcaggcttgg ctcggtcgct gtaagaacct tccaggtaaa
       61 cctcctgcac cctggaaccg gccccctttg caccttctat ataggcacca acaaactctc
      121 tctggggagc accgttctca tcgacgaaac gaccctccac gaatactgac ctgtgaccct
      181 gcaccatttg tgcataagcc atatcaagat gaaagaccgg tccggcagca ccgccggcca
      241 tatttccatt gctgaactga ttgtccccaa ccgaattgat accaaaaacc tcagacatgg
      301 ccgccacttc agatggtaat agaggctgcg ccttttcgct actacctttg cgctccagga
      361 tctcctgaaa gcttttcctg cctgcagggc taagatcatg accacgatcg tagaaattca
      421 gggtcacgtc agcgttagca ggtgcgcaat aggcttccag agaactccgg ggctccccgc
      481 tgtaactgag cggcgcccta acccagtcgg gcggcggcgc aagctttagg aaaccctctt
      541 ttggcgagga attgccggtc aactccatcc ccccttttgc caaaacatca agagaattca
      601 gtcgctcatg attagcagga agatctccct ctccccggaa actcttcaac tccagatcag
      661 cttgtacata taattcaggc atagaacatt tcccctaaga acaaccatta tagagatccg
      721 aaacactcat ccagccgtca ttacggctgc cccactgatt gtcgatctga acccgcggcg
      781 gattcgtcga agtatcaata tcgctgatgc atatcacatg attaatgcca ttgttgggac
      841 tgccacctcc aaacacgggg tctgagccat taaccgcaag aattatcggc aagtcgttgt
      901 tttccttaag ctccaacaac ttattggtca aatccttctc gttgttaaaa gcgatgatac
      961 tgccgtcctg ccagttggca ttagcatcaa gcatcaccac cgtgttatca ccgggcaaaa
     1021 gtctttcagc ttcatcggca atttctccca cagtcaatcc gccgaaaggc tctaaaatcc
     1081 gccccgaaga atcggtccag tattcaccgg tatcagaccg gctggccggg cgccgctgcg
     1141 aaaagcaaag cggcggattc tcgcgctgtc caaggtcatt gagagcagtc acctggtaaa
     1201 gttgcgaagc gaagctgcga gcgccatctt gaggcggaaa agtgcgctcc tcggcgccgg
     1261 gaattagaga ttgcgcgtca atcttaatct gctttccatc cggcgccgtc cactttccgg
     1321 tcaatgctac ttccgaaatc atagatgcgg cttcaccagg tctatcggtg aacgccatgt
     1381 tctcaattgt ggccacattg caagtaccat gcgcaccctg atctatagat tccggatgag
     1441 cagcatgacg catgattccc tgcgcggcat tgattctttc cgcttcaggc acggccccgg
     1501 tctcactatt gagtagccgg gtggtagcct ccatggtctt aacgacttcc tccacgggaa
     1561 gcccttcttc tttagctctt ttctcaaagt cggcacagtc ctgcacaaat ctctgcctgg
     1621 cagcaggatc ttctatctgt gccgaagacc gctccagttc ctggcgcgcc tgttccacag
     1681 agccctcacc tggtttggga ggttcttccg gctccggtct gggcggctcc aacggttccg
     1741 gcgctggttc cggcttgggt tccggtttgg gctccggcgc tggctccgg
//
LOCUS       DAOQNZ010000951         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47734_length_1788_cov_3.694172, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000951 DAOQNZ010000000
VERSION     DAOQNZ010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47734_length_1788_cov_3.694172"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKW73_15180"
     CDS             <1..863
                     /locus_tag="PKW73_15180"
                     /inference="COORDINATES: protein motif:HMM:NF018540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HNA74689.1"
                     /translation="EELDLTNMLDTASDGEIRAELAAVAPARLYHIQKSRGYSTERPT
                     LSNQAIKRLAALVKEVDAGAHVFVDNCYGEFVEEHEPVALGADLMAGSLIKNPGGGLA
                     LTGGYVAGKKTLVENSLIRLTAPGIGGHLGVTFNQNRLVFQGLFLAPSVVSGAVKGAM
                     LAASCLARLGLKVDPSPLARRFDIIQAIEFGNRQRLINFCRALQSYSPVNAHVAPEPS
                     GMPGYDDEVIMAGGSFVEGATIELSADGPLREPYAAYLQGGLSYLHVKMALLGALNQC
                     EAAAPSSFLP"
     gene            1079..>1788
                     /locus_tag="PKW73_15185"
     CDS             1079..>1788
                     /locus_tag="PKW73_15185"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNA74690.1"
                     /translation="MPARQQGLSGAKLDLKILSGTANPELAKEVAEHLGLELTPLKIS
                     PFSDGEIYVQLQESVRGVDCFVIQPTCTPVNENLMELLILLDALKRASAGRITVVMPY
                     YGYGRQDRKAAGREAITAKLIADLLTTAGAQRVVALDLHAPQIMGFFNILVDHLYASP
                     VLLDYVRSLNLKDIVLVSPDVGGVSRTRAFAKKLDDAPIAIIDKRRTKHNVAEVMSVV
                     GEVEGKTAIMVDDMVDTAG"
BASE COUNT          365 a          500 c          498 g          425 t
ORIGIN      
        1 acgaagagct tgatctgaca aatatgcttg ataccgcaag tgatggcgaa atccgtgccg
       61 aactggcggc ggttgctccg gccaggctct accacattca gaaatcccgc ggctattcca
      121 ccgagcgccc caccctttcc aatcaggcca taaagcgcct ggccgccctc gtgaaagagg
      181 tggacgccgg ggctcacgtt tttgttgata actgttatgg cgagtttgtg gaagagcacg
      241 aacccgtggc tcttggcgcc gatctcatgg ctggaagctt gattaagaac cccggcggcg
      301 gactggctct gaccggcggc tatgtggccg gcaagaagac cctggtggaa aattccttga
      361 tcagactgac agctcccgga attggcgggc acctgggcgt caccttcaat cagaatcgcc
      421 tcgttttcca ggggctcttt ctggctcctt ccgtagtttc cggtgccgtg aaaggggcca
      481 tgctggcggc ctcttgtctg gccaggcttg gtttaaaggt ggatccctcg ccgcttgccc
      541 ggcgcttcga cattattcag gcgattgaat tcggcaacag gcagcggcta ataaactttt
      601 gccgggcttt gcagagttat tcgccggtca atgcccatgt ggcgccggaa ccaagtggta
      661 tgcccggtta cgatgacgag gtgattatgg ccgggggcag ttttgtggaa ggggccacta
      721 tcgagctttc agccgatggc ccgctgcggg agccctatgc ggcctatttg cagggcggac
      781 tttcttatct tcatgtgaag atggcgctcc ttggcgccct caaccagtgc gaagcggctg
      841 caccctcctc cttcctgccc tgaagccggg ggtctgtcaa gggtgccagt ttgcattaaa
      901 tctcaaagaa gcagtctgat attagcttct attccccttt aacctttgct aaatgagagc
      961 ttagagcttc ttttcttctt tgcgggtgcc tataattagc ccattatctt taaactgacc
     1021 ttcagttaca gccgtcagcc aaacttaatt tatgaggtta atgccagtgg ttgctcagat
     1081 gcccgccaga cagcaaggct tgtcaggcgc caagctcgat ctcaagatcc tctccggcac
     1141 cgccaatccg gaactggcca aagaggtggc ggaacacctg ggcctggagc tgacgccgct
     1201 taaaatctcg cctttttccg acggcgaaat ttatgtgcag ttgcaagaga gcgtcagggg
     1261 cgtcgattgc ttcgtcattc agcccacctg cacgccggtc aacgaaaact tgatggaact
     1321 gctcatcttg ctcgatgccc tcaaacgagc ctcggccggt cgcatcaccg tggtcatgcc
     1381 ctattacggc tatggtcggc aagacaggaa ggccgccggt cgtgaagcca tcaccgccaa
     1441 gctcatagcc gatctgctca ccaccgccgg cgcgcaaaga gtggtggctc tcgacctgca
     1501 tgctccgcag attatgggct ttttcaatat tcttgtggat cacctctacg ccagccccgt
     1561 gcttttggac tatgtgcgtt ctctcaattt gaaagacatc gtgctggtca gccccgatgt
     1621 gggtggtgtt tctcgtacca gagcctttgc caagaaactg gacgacgctc ccatcgccat
     1681 tatcgacaaa cgccgcacca agcataatgt ggcggaagtg atgagtgtgg ttggtgaagt
     1741 ggaaggcaag acggccatca tggtggacga catggtcgat actgccgg
//
LOCUS       DAOQNZ010000952         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47815_length_1787_cov_2.834873, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000952 DAOQNZ010000000
VERSION     DAOQNZ010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47815_length_1787_cov_2.834873"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..745
                     /locus_tag="PKW73_15190"
     CDS             257..745
                     /locus_tag="PKW73_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74691.1"
                     /translation="MSTFLMILLSVILVIGGLIFFGLMWLKAKAQKGIALLAATAVSA
                     ALQSMKTHQAKPENAGDTQLADLIARFEEGDEKARAAFKSGNYEDVMTIAEPLMEDFA
                     AYKKAMAEKQAAQQQADTGTVIDAEVVEAPKELQAPEAKPADPASNPPAADSAPDADK
                     KN"
     gene            complement(1048..1533)
                     /locus_tag="PKW73_15195"
     CDS             complement(1048..1533)
                     /locus_tag="PKW73_15195"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNA74692.1"
                     /translation="MELKVWLTMETVAVVLLSQLLPCRASDRIDNFVVQDSANIRCIG
                     IGRGKRQAPSESLLEAELYQWLLDCKIRISKPAKSLQKLTGKTSAKFFIEIDKQDKSA
                     RLELTQSSGSKTFDQQAQDVIRKASPFKEPFNDLPYRRGLLIEISPSIVTVKLASNKP
                     G"
     gene            complement(1595..>1787)
                     /locus_tag="PKW73_15200"
     CDS             complement(1595..>1787)
                     /locus_tag="PKW73_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74693.1"
                     /translation="YVPVDKAEVIEKTLPDGKKVLLADFRKRKAHSFSNTPVVVLIQF
                     RTFSEVALREGDWSELTSH"
BASE COUNT          458 a          526 c          393 g          410 t
ORIGIN      
        1 cctgccgatc tcaactcagg tctcgatgca atcatctagg ctctgaacag gccggacagg
       61 acaacaacaa catctgacta ctttgagcag cgaccagcaa ccttcaagcc ggtcgctgct
      121 cttttctttc gcctgaaacc tttccagggc aaggcgaaag ctatgtccct gaggcgttca
      181 gcctctcaaa cggtaggagt cacagcactc ttgccacccg cggtcaaaag accgcattcc
      241 atccctggag caaacaatga gcactttcct catgatcctt ctgtcagtca tcctggtcat
      301 cggtggactg atcttcttcg gcctgatgtg gctcaaagcc aaagcacaaa agggcattgc
      361 ccttctggcc gccaccgctg tcagcgctgc cctgcagagc atgaagactc accaggccaa
      421 gcccgagaat gccggtgaca ctcagctcgc cgacctcatc gcccgcttcg aagaaggcga
      481 cgagaaagca cgcgccgcct tcaagagcgg caactacgaa gacgtcatga ccatcgcgga
      541 accgctgatg gaagacttcg ccgcctacaa gaaagccatg gccgaaaaac aagccgccca
      601 acagcaagcc gacaccggca ctgtcatcga cgcggaagtt gtggaggcac ccaaggagct
      661 gcaagctccc gaagcgaagc cggctgaccc tgcgtccaac ccgcccgctg cggattctgc
      721 gccggacgcc gacaagaaga actagaccag gcaccttggc ttacccggga cgagccgctc
      781 agccgccgcc cttgaggaag ccaactgccc agatctaatc tgaccccgtt ccacgcgggg
      841 aagaaggagt catcctcttc cccgcttttt tcaaacaatt tccctcgcct cagtacttaa
      901 tgcaggcagc gccgaagaca ggcactggtt cagtccttta atcattaccg caggtttact
      961 atatcacacc gtgctctacc cccaaaataa cactcaggga agtctgattt tactcaaagt
     1021 taattgaaac cgtatcggca tgatgcgcta ccctggtttg ttacttgcca gttttacagt
     1081 tacgattgaa ggactaatct ctattaacag cccgcgtcta taaggcaaat cattgaatgg
     1141 ttccttaaac ggactagcct ttcgaataac atcttgtgct tgctgatcaa atgtctttga
     1201 accagacgat tgcgtcagct ccagacgagc ggacttgtct tgtttatcta tttcaataaa
     1261 aaacttcgcc gaagttttac ctgtcagttt ttgtaggctt tttgcaggct ttgatattcg
     1321 aattttacag tccaagagcc attggtaaag ctccgcctca agtagagact cagatggagc
     1381 ttggcgttta cctcgtccaa tccctataca acggatgttt gctgaatctt gtaccacaaa
     1441 gttgtcgatt cgatcacttg cccggcaggg cagcagttgc gacagcaaga ccaccgctac
     1501 ggtctccatt gttaaccaca ccttaagctc catttcaatc tcacctaaca gaggcaatta
     1561 cagttgcgaa gcaggcacag cagctgtaca caacctagtg gctcgttagc tcagaccaat
     1621 ccccctccct caaggcgact tcagaaaatg ttctaaactg aataagaact actacaggtg
     1681 tatttgaaaa tgagtgcgct tttctttttc tgaagtcggc cagcaagacc ttcttcccat
     1741 ccggcaaagt cttctcaata acctctgctt tatcaactgg aacataa
//
LOCUS       DAOQNZ010000953         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47862_length_1786_cov_3.621606, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000953 DAOQNZ010000000
VERSION     DAOQNZ010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47862_length_1786_cov_3.621606"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKW73_15205"
     CDS             complement(<1..186)
                     /locus_tag="PKW73_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74694.1"
                     /translation="MQKTQLAAALIAVLGLSFNGMARAEGSDAEGKSEVKTEKKAAKE
                     CKKECKKGKEASCKGKEG"
     gene            complement(402..782)
                     /locus_tag="PKW73_15210"
     CDS             complement(402..782)
                     /locus_tag="PKW73_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74695.1"
                     /translation="MKRIAKIEAAALVTALSLSFGLAPAFAGDAASGKTEEKSKDKMA
                     AKETKKEGKQGADSSCGEGSCGTDKKGAAAAKAKAKVKAKAAAKVKEVKKAAKQGADS
                     SCGEGSCGTDKKGAKAAEEKHKAK"
     gene            871..1488
                     /locus_tag="PKW73_15215"
     CDS             871..1488
                     /locus_tag="PKW73_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74696.1"
                     /translation="MTQTTVANYFQQTLREWVVNGKFPETTVEACSPSLATKLGYTAE
                     TFTFDNYIHSSQTPEWSLRPLDFGPDLGGTLYIYLPTTWADLTKLDNATQVLSGGLPR
                     QTLYLMDKPEWSGTLEAFFNQGQVGAFVYYLIQPPDFHQWICLMEDGNRVWLFAFYSA
                     PGKLLITKADASDKASLELQLTSLINSNPWYGFVSTASTTSAGTV"
     gene            1501..>1786
                     /locus_tag="PKW73_15220"
     CDS             1501..>1786
                     /locus_tag="PKW73_15220"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HNA74697.1"
                     /translation="MPVLYLHGFASGPSSRKARFFHDQFSKRQVPCVVPDLNYPSFFD
                     MTLNSQLALVRQEAHALRERFDDKLIVIGSSMGGLLAAMLSDEIDFVEKMY"
BASE COUNT          421 a          491 c          386 g          488 t
ORIGIN      
        1 gccttcttta cctttgcaag aggcttcttt gcctttcttg cattcttttt tgcactcttt
       61 ggcagctttc ttttcggttt tcacttcgct cttgccttcg gcatcagagc cttcggcgcg
      121 agccatacca ttaaaagaaa gaccaagaac ggctataaga gctgctgcta gttgagtctt
      181 ctgcatttcg acctccggaa acggtaacaa atcatattca ctaacatata cgccgacaac
      241 ctcaaatatg aatgaagtgg atcaggcgct atggccatca tgctaggcca ggttgattaa
      301 aaaaaaccaa attgctggtt acggctgtgt taaatctgaa ggcatgcagc ctgtgcgccc
      361 gtgcgccgaa aaataactta acaaaatccg ccagcccgac cttacttggc cttgtgtttt
      421 tcttcggcgg ccttagctcc tttcttatcg gtaccacagc taccttcacc acaagaggaa
      481 tcagcaccct gcttggcagc cttcttcact tccttcactt tggcagctgc tttcgctttt
      541 accttggctt tggccttggc cgctgccgct ccctttttat cggtcccaca acttccttca
      601 ccgcaagagg agtcagcgcc ctgttttcct tccttcttgg tttctttggc agccattttg
      661 tccttgctct tctcttccgt ttttccactg gcggcgtcgc cggcaaaggc aggcgccaga
      721 ccaaaagaca gactgagggc agtaacaagg gcagcagcct cgatctttgc aattctcttc
      781 atagaaatat ctccaattta gaacgaacta tattattgct cagctaaaat ctgtaagctt
      841 ataacccaga ggcgtgatgg aaccaggtag atgactcaga cgacagttgc caactatttt
      901 caacaaactc tcagggagtg ggttgtcaac ggaaagttcc cagagaccac agttgaagcc
      961 tgttctccgt cgctagcaac aaaactgggc tacacggccg aaaccttcac cttcgataac
     1021 tatattcatt ccagccagac tcctgagtgg agcttgagac ctctggattt tggaccggat
     1081 ctgggcggca ccttatacat atatctgccg acaacctggg ctgatctcac taaacttgac
     1141 aatgctactc aggtgctgag cggcggtcta cccagacaaa ctctatatct aatggataag
     1201 ccggagtggt caggcaccct ggaagcattt ttcaaccagg gtcaagtcgg tgcctttgtc
     1261 tactatttga ttcagccgcc ggacttccac cagtggatct gccttatgga agatggcaat
     1321 cgggtctggc tcttcgcctt ctattccgcc cccggtaaat tgcttatcac caaagctgat
     1381 gccagcgata aagcctcact ggaattgcaa ctgacttccc tgatcaattc caacccctgg
     1441 tatggcttcg tcagtacagc ctccactaca tcagccggta cggtgtaata ggcaggcaga
     1501 atgcccgtac tttatctgca cggcttcgcc tccggacctt cctccagaaa agcccgattt
     1561 tttcacgatc aattcagtaa acgccaagta ccttgcgtcg taccggattt gaactatcca
     1621 tccttctttg atatgaccct gaatagccag ctggctctgg tcagacaaga agctcatgca
     1681 ttgcgggaac gattcgatga caagctcatt gtgattggtt ccagcatggg tggactactg
     1741 gccgccatgc tctcggatga aatagacttc gttgaaaaaa tgtact
//
LOCUS       DAOQNZ010000954         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47881_length_1786_cov_2.667244, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000954 DAOQNZ010000000
VERSION     DAOQNZ010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47881_length_1786_cov_2.667244"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PKW73_15225"
     CDS             <1..764
                     /locus_tag="PKW73_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74698.1"
                     /translation="GGETKPEPRPEPKPEPLPKPEPKPEPLPQPEPLPKPEPLPKPEP
                     KPEPIPQPGDGNLPKPGVDTPSKPTDGSDLYNSFRIEAGVSQQDVAQVKDALRNVPEP
                     VRQAMRDLHPTVKIVNNMPSFASGAAAYWDPGNNEIVVAARTGMVKEAASHEFWHVAD
                     TRYGITDDPGFRNAAAKDIRNGAVSKLGRAAGVLVDDPEVNGVPVGDAFAEVGVDLDR
                     EYGGDSDQTGIAGAMGEAFSNTRAWLSQWVKKNLK"
     gene            803..1192
                     /locus_tag="PKW73_15230"
     CDS             803..1192
                     /locus_tag="PKW73_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74699.1"
                     /translation="MSELSASVNGKVETLVDKVKHNEPLTEELNSVKFEDRLNLAKEV
                     KARMEQEQKQDASLPTLELTVGQDTQKRPHLVDIKSVASGKSQDLYDLPGKIEAQPLP
                     IDLKLNLDAGHSRYLKTGGIESIRPQN"
     gene            1249..1656
                     /locus_tag="PKW73_15235"
     CDS             1249..1656
                     /locus_tag="PKW73_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74700.1"
                     /translation="MLDSLSEQELQLNYLCAAMKRSMPVAAELNSLPFEERLSLALAA
                     IERLSTERLENKSIPLVMFKTGLDSRGRRHLLQIDCITLASSAVVYELPGTISKQSLA
                     DEMKLNLDAAHSRRLKTQGLKILMPLADLSCSA"
BASE COUNT          461 a          414 c          507 g          404 t
ORIGIN      
        1 cgggtggtga aaccaagccg gaaccaagac cggaacctaa accggaaccg ttacccaagc
       61 cggagcccaa accggagcca ttgccgcagc cggaaccttt acccaagccg gaacctctgc
      121 ctaagcctga accaaagccg gagccaattc ctcaacccgg agatggaaat cttcccaagc
      181 ccggagtgga tacgcccagt aagcccactg acggcagtga cctctataac agcttccgga
      241 ttgaggcagg agtctcccag caagatgtgg cgcaagttaa agacgcactg cgcaatgtac
      301 cggaacctgt aaggcaagcc atgcgagatc tgcatccgac cgtaaagatc gtcaacaata
      361 tgccttcttt tgcctccggg gccgctgcct attgggaccc cggtaataat gaaatcgttg
      421 tcgccgctcg caccggtatg gtgaaggaag ctgcttctca tgaattctgg catgtggctg
      481 ataccagata cggtataacc gacgatcctg gattcaggaa tgcagcggcc aaagacataa
      541 ggaatggagc tgtttctaaa ctaggtagag ctgccggtgt gcttgtagat gatccagaag
      601 tgaacggcgt gcctgtgggt gatgcttttg cggaagtggg tgtcgattta gaccgggaat
      661 atggtggaga tagcgaccag accggtatcg ctggtgccat gggagaagcc ttcagcaata
      721 ccagagcctg gttgagtcag tgggttaaga aaaacttgaa gtaagtgtta ccggtcgttc
      781 aagtcaaaag aggatgcgga aaatgtcgga actgtcagcg tcggtgaacg gaaaagtaga
      841 gactttagta gataaagtca agcacaatga gcctttgacc gaagaactca atagcgttaa
      901 gtttgaggac cgtctcaacc tggctaagga ggtgaaggcc aggatggagc aagaacagaa
      961 acaggatgcc tccctgccta ccctggagtt gacggtgggg caagatacac agaagcgtcc
     1021 gcacttagtg gacattaagt cggttgcgtc cggcaagtct caggaccttt acgatttgcc
     1081 cggcaaaatt gaggcacagc cattgccgat tgatctgaaa ttgaacctgg acgccggtca
     1141 cagtcgctat cttaagaccg gcggtataga gagtattcgt ccgcaaaact agaagcggtg
     1201 tgtgtagcgg gcgcggcatt gttgtgttgg aagcgggagg gaagcaatat gctggattca
     1261 ttgagcgagc aagaactgca attgaattat ttatgcgcgg ccatgaagcg ctcaatgccg
     1321 gtggccgctg agttgaattc tttgcctttt gaagagcggc tgtcgcttgc tctggcggca
     1381 attgagagac tatcgactga gcgcctggag aataagtcga ttcccttggt tatgttcaag
     1441 accggtcttg atagcagggg gcgaagacat ttgctgcaga tcgattgcat cacgttggca
     1501 agttcggcgg tagtctatga attacctggt accatcagta aacaatctct tgccgatgaa
     1561 atgaagttga accttgatgc tgcccatagt cgccgtctca agacgcaggg cttgaaaatc
     1621 ttgatgcccc tggctgattt atcctgctct gcttaaatat cacctgccgg cgcttatttg
     1681 attgatgcgt tttgactgga ttttatgctc tttgagggat ccttgactct agagactctc
     1741 tctaggctca ggggaccggc gtgaaaaagg gcttactaaa tgaacg
//
LOCUS       DAOQNZ010000955         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47924_length_1785_cov_2.619653, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000955 DAOQNZ010000000
VERSION     DAOQNZ010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47924_length_1785_cov_2.619653"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /gene="pdxS"
                     /locus_tag="PKW73_15240"
     CDS             <1..737
                     /gene="pdxS"
                     /locus_tag="PKW73_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNA74701.1"
                     /translation="DIRADGGVARMSDPELISQIIEAVSIPVMAKARIGHFVEAQILQ
                     SLGVDCVDESEVLTPADEQYHIDKNQFDIPFVCGAKNLGEALRRIGEGAAMIRTKGEA
                     GTGDVVEAVRHARTILGEIRRLTVLPKEELMTYAKEIGAPFELVKQVAEEGKLPVVNF
                     AAGGIATPADAALMMQIGVEGVFVGSGIFKSGDPMKRACAIVKATTFYKDPEVLAKVS
                     RNLGEAMVGRSARTLVGDETLAARGW"
     gene            754..1401
                     /gene="pdxT"
                     /locus_tag="PKW73_15245"
     CDS             754..1401
                     /gene="pdxT"
                     /locus_tag="PKW73_15245"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF013350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNA74702.1"
                     /translation="MTKTDRRPLIGVLALQGDFSEHEARLREAGADTRQIRYASQVAD
                     LDGLVIPGGESTAIARLTEEGAAVIFATVKERILSGMPVWGTCMGSIFLAEEIEGSAQ
                     GRLGVMSIKVRRNAFGPQKKSFQCPLAITGLGEPFPAVFIRAPLITAVGDGVEVLGVL
                     PAELNAGASGIVMARQKNMLVTAFHPELTDDLRVHAYFLQMVAECLSRPLASAEV"
BASE COUNT          372 a          467 c          515 g          431 t
ORIGIN      
        1 ccgatattag agcggacggc ggcgtggcca gaatgtccga ccctgagctg atcagccaga
       61 tcatcgaagc ggtttccata ccggttatgg ctaaagctag aatcggacat tttgtggaag
      121 cacagattct gcagtcgctt ggagttgact gcgtggatga aagcgaagtc ttgactccgg
      181 ctgacgaaca gtaccatatc gacaaaaacc agtttgatat ccccttcgtc tgtggtgcca
      241 aaaacctcgg cgaagccctg cgccgcatcg gcgaaggtgc tgccatgatc cgcaccaagg
      301 gcgaggccgg taccggcgat gttgtggaag ccgtgcgtca tgcccgcacc atccttggtg
      361 aaatcagaag actgaccgtg cttcccaagg aagaactgat gacctatgct aaagaaatag
      421 gcgccccctt cgagctggtc aagcaagtag cggaagaagg caaattgcct gtggtcaact
      481 tcgccgccgg cggcatcgct acaccggctg atgctgcttt gatgatgcag attggcgtgg
      541 aaggagtatt tgtgggctcc ggtatcttca aatcagggga tcccatgaaa cgggcctgtg
      601 ccatcgtcaa agccaccacc ttctataaag acccggaagt actggccaag gtcagccgca
      661 atcttggtga agccatggtg ggtcgctcgg cgcgcaccct ggtcggtgat gaaaccctgg
      721 ctgcccgcgg ctggtaagag gaaacctgta actatgacga aaactgatcg ccgccccctg
      781 ataggggttc tcgccctgca gggagatttt agcgaacacg aggccaggtt gagggaagct
      841 ggtgccgaca ccagacagat tcgctacgcc tctcaagttg ccgaccttga cggtctggtc
      901 attcccggcg gtgagtccac cgccattgcc agactgacgg aagagggggc ggcggtcatt
      961 ttcgcaacag ttaaggagcg cattctcagt ggcatgccgg tctggggcac ctgcatgggc
     1021 tccatttttc tggctgagga aatcgaaggc agtgcgcagg gtcgcctcgg cgtcatgtcc
     1081 attaaagtgc ggcgcaatgc tttcggtcca cagaagaaaa gctttcaatg tcctctggca
     1141 atcaccggtc tgggagagcc tttcccggcg gtcttcattc gagcccctct cataacggct
     1201 gttggtgatg gcgtggaagt attgggtgtt ctgccggcgg aactgaatgc cggtgcctca
     1261 ggcatagtta tggccaggca gaaaaatatg ctggtgacgg cttttcatcc ggagttgacg
     1321 gacgatctgc gtgtccatgc ttactttctg caaatggttg ccgaatgcct ttctcgccca
     1381 cttgcgagtg ccgaagtatg aaacaactag tagttgcgat ttcgcttagt gtactgattt
     1441 cggctcttgt cggcggtttg atttgcacac agataggctc cttatactcc agctcatatg
     1501 tgttgtgcgc tgccgccccg tcctcaactg acacagcttc cggtccgtcg ttcgatataa
     1561 atgcttttct ggaacgagcc aggaggcggg ctaagcctac tccacaggaa cttttgttgc
     1621 gccacggaac ttctcttatg gcactcttct tgtctgaagt gctggcgctt ttttggttgc
     1681 tttcaaagcc ccctcaggtg gcgggggtgc gaaaggtttt tgttaccgca agcctctttc
     1741 ttgtgcctgt agtgcttgcc gtgttggcat tttattatgc gatct
//
LOCUS       DAOQNZ010000956         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47970_length_1784_cov_3.235396, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000956 DAOQNZ010000000
VERSION     DAOQNZ010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47970_length_1784_cov_3.235396"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKW73_15250"
     CDS             complement(<1..536)
                     /locus_tag="PKW73_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74703.1"
                     /translation="MNRLPPNKPSENTPSGGPRRLRLPAESACPALSKVRVLLEEAQS
                     KRGTLIEMPWSQPDHKSFIITVQWDETLKDPVWTLYEEIEGTSKVVWTQPFAAGDLEF
                     MYDILSMSAGSTSGGLAIPDELKIKPVEAPRMSDLDKFNEAPAAAPMGGGLGGGLGGG
                     LGGGLGGGPEQRPQPWQHP"
     gene            complement(609..>1784)
                     /gene="hmgA"
                     /locus_tag="PKW73_15255"
     CDS             complement(609..>1784)
                     /gene="hmgA"
                     /locus_tag="PKW73_15255"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNA74704.1"
                     /translation="GSSFVMPRHENLRSWLYRIRPSVLHSKFKRRDNKLLRSRPFTEE
                     VPTPDQLRWDPFDLPVKDKVDFISSLATLAGNGDYGSVRGAAIHIYAANASMEDSFFY
                     NSDGDFLIVPQLNSLVIATEFGVIEAAPGEIVVLPRGIKFQVKLPQPEARGYVLENYG
                     PPFRLPGLGPIGANGLANPRDFLAPTAAYTKREGRFTLINKFCGNLFEAQLEHSPLDV
                     VGWHGNYFPYKYDLALFNVINTVSFDHPDPSIFTVLTSPSEIPGTANIDFVIFPPRWM
                     VADNTFRPPYYHRNIMSEYMGLIHGVYDAKEEGFLPGGGSLHNCMTAHGPDRDTFEKA
                     SQVKLQPVYQGNTLAFMFESSLVFAPTTLAITTKHRQEDYLSCWRGLPVNFKTPTPA"
BASE COUNT          383 a          474 c          529 g          398 t
ORIGIN      
        1 ggatgctgcc atggctgcgg ccgctgctcg ggtccaccgc caagaccgcc acccagaccg
       61 ccgcccagac cgccgcccag tccaccgccc atgggtgccg ctgccggggc ctcgttgaat
      121 ttgtccaggt cgctcattct tggagcttct accggcttga ttttcaattc atcaggaata
      181 gccagcccgc cggaagtgct gcctgccgac atggacaaaa tgtcgtacat gaattccagg
      241 tcaccggctg caaaaggttg cgtccagact accttggagg tgccttcgat ttcttcgtaa
      301 agcgtccaga caggatcttt cagagtttca tcccattgca cggtgataat gaagctctta
      361 tggtccggtt gagaccaggg catttcaatc aaagtgcccc gtttgctctg ggcctcttca
      421 agcagcactc gcaccttgct caaagcaggg caggccgact ccgccggcaa tctaagacga
      481 cggggtccgc cactgggggt gttttcactg ggcttgttgg gcggtaatct gttcatcgct
      541 tcctcgaaac tctactttct gcactcgtcc tttaattcat atttcacagg ttgcacactt
      601 tataaacgtc aagccggcgt aggcgtcttg aaatttacag gcaacccccg ccagcaggac
      661 agatagtctt cctggcgatg cttggttgtg atggccaggg tggtgggggc gaataccagg
      721 gagctttcga acatgaaagc gagggtattg ccctgataaa ccggctggag cttgacctgg
      781 ctggcctttt caaatgtatc ccggtccggc ccatgggcgg tcatgcagtt gtgcagagaa
      841 ccgccgcccg gcaggaagcc ttcttccttg gcatcataaa cgccatgaat gagacccatg
      901 tactcactca ttatgttgcg atggtagtag ggcgggcgga atgtgttgtc ggccaccatc
      961 cagcggggtg ggaagatgac gaagtcgata ttggccgtcc cgggaatctc cgatggtgaa
     1021 gtgagcacgg tgaaaatgga gggatccgga tggtcgaagc tgacggtatt tattacattg
     1081 aacagggcca ggtcgtattt gtaagggaaa tagttgccgt gccagcccac cacatccaga
     1141 ggtgagtgtt ccaactgggc ttcaaaaaga ttgccgcaga acttattgat cagggtgaac
     1201 cggccttccc tttttgtata ggcagccgta ggtgccagga agtcgcgggg attagccagg
     1261 ccgttggcac cgattggtcc cagtcccggc aggcggaagg gcggaccata gttctccagg
     1321 acataacctc tggcttcagg ctgggggagt ttgacctgaa actttatgcc ccgcggcagc
     1381 acaacgattt ctcccggcgc cgcttcgatg acaccaaact cggtggcgat gaccaggctg
     1441 ttcaattgcg gcacgatcaa aaagtcgccg tccgaattgt agaagaagct atcttccatg
     1501 gaggcattgg ctgcgtaaat gtggatggcg gcgccacgga ctgagccata atcgccgtta
     1561 ccggccagcg tggccaggga tgagataaag tcgaccttgt ctttgaccgg caggtcaaag
     1621 ggatcccagc gcaactggtc gggggtgggc acctcttccg tgaaaggacg ggagcgcagt
     1681 agtttgttgt cacggcgctt gaactttgag tgcagcacgg aggggcgaat gcgatagagc
     1741 caactgcgca ggttttcatg gcggggcatg acgaaagaac tacc
//
LOCUS       DAOQNZ010000957         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_47981_length_1784_cov_2.750723, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000957 DAOQNZ010000000
VERSION     DAOQNZ010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47981_length_1784_cov_2.750723"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1206)
                     /locus_tag="PKW73_15260"
     CDS             complement(178..1206)
                     /locus_tag="PKW73_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74705.1"
                     /translation="MSNAQLTALVRDPSVGSRALSVTVVGSGPLVTVLAEEKEIAQDK
                     DLKIDAMFLARTLIDGAPGQIEKVKVLFSQTGKPARYVTISRGEITDFSAGRVSAATL
                     LQSLALVAVHAENMPEITDGPEVERRLLAWKRIDKLRAQGTGVSPFEAIFKEIQGLAK
                     ANDAEKVSQRLSYLESKLTGQEEQVKQAKDSLRGRGVPAVKTASSASAGAGAGTTAPG
                     GQGAAPPQGAGGGYIPPEAEHLKATFNAQSNEIISQVTRKNSSEGYKLRDLKSEIEKA
                     FAAHQEARAYEMIHNFDLAATKVLGSSAFGPGGQGGGMGPGGGMGQGGGPPGGGPPGG
                     GPPGGGPF"
     gene            complement(1424..>1784)
                     /locus_tag="PKW73_15265"
     CDS             complement(1424..>1784)
                     /locus_tag="PKW73_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74706.1"
                     /translation="AYALRQSEHFSTSAPTNFPIEIYRSNVTATGFLNSTKGSPSASL
                     SIATKDSLEVVSQFYQTVLRNGGWKFQTPSAEAQAKMGQIYMLRAQKDKQNANIMLLN
                     KQGEPGTNISINWYLSP"
BASE COUNT          352 a          495 c          474 g          463 t
ORIGIN      
        1 gtccagtacg gtaccgacca gaggatcgcg agcctttccc ttgttgcgag aatcttcgag
       61 taattcgccg gaactcaaac ggtgttactc cctcaggtca tggctgcaat gcaggcgccc
      121 ggttccttta ttccacggaa tagtgaacca atcatgtttt cttgagtttt ccggaattta
      181 gaatggtccg ccgccgggag gtccgcctcc aggtggtcca ccgccgggcg gtcctccgcc
      241 ctgtcccatg cccccgccag gacccatgcc gccaccctgc cctcccggtc cgaaggctga
      301 ggagcctaag accttggtgg cagcgaggtc aaagttgtgg atcatttcgt aggctctcgc
      361 ctcctggtgc gccgcaaaag ccttttctat ctctgacttc aagtctctga gcttgtagcc
      421 ctcgctgctg ttcttgcggg taacctggct gataatctcg ttgctttgcg cgttgaaagt
      481 tgccttgaga tgttccgctt ccggtggaat atacccgccg cctgctccct gtggcggcgc
      541 agcgccctgc cctccggggg cggttgtgcc tgcacccgca ccggctgaag cgcttgaagc
      601 tgttttgaca gcaggtactc ctcgaccacg cagggagtct ttggcttgct tgacttgctc
      661 ttcttgtccg gtaagctttg attcgagata ggacaatcgc tgagagactt tctcggcatc
      721 attggctttt gccagtccct ggatctcctt gaagatagcc tcaaacgggg aaaccccagt
      781 accttgagcg cgcaatttgt cgatgcgctt ccacgccagc aggcgtcttt ccacttccgg
      841 cccatcagta atttccggca tgttctcggc gtgtacggca accagggcga gcgactgcag
      901 caaggttgca gctgacactc gtccggcgct aaagtctgtg atttcgcccc gggaaattgt
      961 gacgtaacgt gcaggcttgc ctgtctgact gaaaagcact ttcacctttt ctatctgacc
     1021 gggagcgcca tcaattagag tgcgagcgag aaacatcgca tcgattttca aatctttgtc
     1081 ctgggcaatc tctttttctt ctgccaaaac cgtgacgagc ggaccgcttc ctacaaccgt
     1141 aacgctgagt gcgcgtgatc caacggacgg atctcgcacc agagcggtca gttgagcgtt
     1201 tgacaaattg gcttttccag ccgcctgcgc aagcacgctc tgtccgcggc aaacgcccag
     1261 cccgagtgcg ccgattactg ttaggctcat cgcagttgag cgcaggattg ataaggacga
     1321 aaatctggtc atgaagactc taccgttgag tgtggacaaa atcccaatgt tggaatttta
     1381 ccagccctcg gcggtttgcg cgttcggtcg aagaggacgc tgctcaggga gagaggtacc
     1441 aattaatgga gatattcgtt ccgggttcgc cttgcttatt tagaagcatg atgttcgcat
     1501 tctgcttatc tttctgcgct ctcagcatgt aaatttgccc catcttcgct tgggcttctg
     1561 ccgaaggcgt ctggaatttc catccgccgt tgcgcaatac cgtttggtaa aactgagaaa
     1621 caacctccag gctgtctttt gtcgcaatgc tcaaactggc tgaaggtgag ccttttgtag
     1681 agttcaagaa gccggttgcc gtgacgttgc ttctatatat ttcgatagga aagttagtag
     1741 gagcagaagt agaaaagtgt tcactttgtc ttagtgcata tgcg
//
LOCUS       DAOQNZ010000958         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48047_length_1783_cov_2.418981, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000958 DAOQNZ010000000
VERSION     DAOQNZ010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48047_length_1783_cov_2.418981"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKW73_15270"
     CDS             <1..209
                     /locus_tag="PKW73_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74707.1"
                     /translation="ATYSILRFHHERNQWNGGRSPYNVRWKALPYNVLFGFRHREEIM
                     AAPRTGDIIVVNHFRPAQTGWRLA"
     gene            258..566
                     /locus_tag="PKW73_15275"
     CDS             258..566
                     /locus_tag="PKW73_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74708.1"
                     /translation="MYYLALLKERINQKLRHLAFLLISLILSDKALGVVAFKETITGG
                     FFLVLFSATGDLFSLFMALWSIATIALVVFAYLRRDQMLAFCRKWQQGQTASKAIAAS
                     "
     gene            664..1098
                     /locus_tag="PKW73_15280"
     CDS             664..1098
                     /locus_tag="PKW73_15280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA74709.1"
                     /translation="MIQIANPDDTEEISLLNRQLRIDLPCSFPWDTPEWIAEEIAKGN
                     YYVYRDREGIAGALCLRTDSQNGNIEAMAIRSNTHGQGIGRKLVEFAVSRCRLLNIGT
                     LSVHSFCLYGVRPFYEKCGFTCVGDNRTYHEVPYYSFSMSLK"
     gene            1131..1292
                     /locus_tag="PKW73_15285"
     CDS             1131..1292
                     /locus_tag="PKW73_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74710.1"
                     /translation="MTDTKPEIENLPELPPTLAALLPLVRAMTVLQAYAELHGEQLDI
                     FSLHPRTKA"
     gene            1289..>1783
                     /locus_tag="PKW73_15290"
     CDS             1289..>1783
                     /locus_tag="PKW73_15290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA74711.1"
                     /translation="MNNPYLLLFREALKQPDAHDDWDLQWSLAHRFSWAVPTERTIRE
                     IRDFSPLGIVEIGAGTGYWASLLKESGADVIAYDSNPVELGSNRYHRNEKTSEPVKSF
                     TTVLAGGARAVVRHPERALMLCWPPHDWNANTPYAVRSMSNRALAHFKGDRLIYIGQP
                     EGGLT"
BASE COUNT          418 a          517 c          444 g          404 t
ORIGIN      
        1 ccgcaaccta ctcgattctg cgctttcacc atgaacgcaa ccagtggaac ggaggcaggt
       61 ctccctacaa cgtgaggtgg aaagcgctcc cttacaacgt gctgttcggc tttcgacatc
      121 gggaagaaat catggccgca ccaagaaccg gcgacatcat cgtcgtcaac cacttcagac
      181 ctgctcaaac cggctggagg ctggcttaac aggatcaaac ctcctaaaca ctaattactt
      241 agtaaggggt ctactccatg tactacctag ccctactgaa agaacgcatt aaccagaagc
      301 ttcgtcacct ggctttcttg ctcatatcac tgatcttgag cgacaaagct ctgggggtgg
      361 tggcattcaa agaaaccatc acgggaggct tctttctcgt tctattctct gccacaggcg
      421 acctcttcag cctcttcatg gcactctggt ccatcgccac cattgccctg gtggtgttcg
      481 cctatctgcg tcgggaccag atgctggctt tctgtcgcaa atggcagcag ggacagacgg
      541 cctcaaaagc aatcgctgct tcgtaaaaca acccgtggtt gcacgcgcct tctcgatcag
      601 actcctggtc gacaggcggt gcagccgctc actttgtaat gtcttgcccc atgggaggca
      661 cctatgattc aaatcgcaaa tccagatgat actgaggaaa taagcctgct caaccggcag
      721 cttcgcatcg accttccctg ctcctttccc tgggatacgc cggagtggat cgcagaggag
      781 atcgccaagg gtaattacta tgtctaccgc gaccgggaag gaatcgcagg ggcactttgt
      841 ctgcgcactg attcccagaa tggcaacata gaagctatgg ccatacggtc aaacacacac
      901 ggacagggaa tcggccgcaa actggtggag ttcgctgtca gccgctgccg gctcctcaat
      961 atcggcactc tttcagttca ttcattctgc ctgtacggcg ttcgcccctt ctacgagaag
     1021 tgcggcttta cctgtgtagg cgataaccgc acatatcacg aagtccccta ttacagcttc
     1081 tcaatgtcgc tcaaatagga ccgtctgatc cagcaagaga ggtaaatcat atgaccgaca
     1141 ccaagcctga aattgaaaat ttgccggagt tacctccgac tttagcagcg ctcctgccgc
     1201 tggtaagagc catgactgtt ctccaggctt atgcggaact gcacggcgaa caactggaca
     1261 tcttttccct tcacccccgg acgaaagcat gaataatccc tacctgcttc ttttcagaga
     1321 ggcgctaaag cagccggatg cccatgacga ctgggatttg cagtggagcc tcgctcatcg
     1381 tttttcctgg gcggtgccga cggaaagaac gatccgggaa atcagagact tcagtcccct
     1441 ggggattgtc gaaatcggtg ccggcaccgg ttactgggca agccttttga aagagagcgg
     1501 cgccgatgtc atcgcttacg attctaaccc tgtcgaactt ggctccaacc gatatcaccg
     1561 aaacgaaaag accagcgaac cggtgaagag cttcacgacg gtcttagccg gaggtgcccg
     1621 agccgttgtt cgccatcctg aaagagcgct gatgctgtgc tggccgcccc acgattggaa
     1681 tgctaatacc ccttatgccg tgcgttccat gtctaataga gccctcgctc acttcaaggg
     1741 tgaccgtttg atttacatcg gtcagccgga aggcggttta acc
//
LOCUS       DAOQNZ010000959         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48236_length_1779_cov_4.699536, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000959 DAOQNZ010000000
VERSION     DAOQNZ010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48236_length_1779_cov_4.699536"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PKW73_15295"
     CDS             <1..1011
                     /locus_tag="PKW73_15295"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA74712.1"
                     /translation="VLGLTMDEVVNMIRGKAGTGVEIKVARTVGDTVEARTFALTRSN
                     LKVPMLETRKMGDIVYMKLRSFSAGYTSQEVMESLYTACTGKNLPMESTEVLAVTKSY
                     VPERDCALTGLVLDMRANPGGRMEQAMDIAHMLLVEGPTITTLTRDGDSIAELREVLF
                     NDHNARITVKNGEILQSKNEPRVFRLFPDNRPIVVLVDGNSASASEILAGLLQRNGRA
                     LIVGEPSFGKTVGQNVVPLVFGTGLKITTFSFLPGGAELGVAVLPDREVAASDAFKDD
                     PIQAEDVQLTAAVNAIAELNGKVEALAKLKARVQEEHSRRDSRMLDAQGRGLELIPVP
                     AK"
     gene            1053..1538
                     /locus_tag="PKW73_15300"
     CDS             1053..1538
                     /locus_tag="PKW73_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74713.1"
                     /translation="MKAIFDPIFLFALTVAAAYCGYFVNKVRGWFTTRGALAVTVFGA
                     VMYLGLIFGMGFGNEGGLYVLSFGFGALLSCMAPRYVAIILKPFAVTFGLLRRFVALV
                     SKPVRARADSSDATHPTSSFLLVFLHTAETFFGDMENLFGKYAERHPAASPLQPGVGA
                     K"
BASE COUNT          390 a          479 c          506 g          404 t
ORIGIN      
        1 gtgcttgggc tgaccatgga cgaagtcgta aacatgattc gcggcaaggc cggtaccggt
       61 gtagagataa aagtggcgcg cactgtcggt gataccgtcg aggcgcgcac ttttgctctt
      121 acccgcagta atttgaaagt gccaatgctg gagacccgca agatgggtga catcgtctat
      181 atgaagctga gaagcttcag cgccggttat accagccagg aggtgatgga gagcctgtac
      241 acggcttgca cgggcaagaa cttgcctatg gagagcacgg aagtgcttgc cgtaaccaag
      301 agttatgtgc ctgagcgtga ctgtgccctg accggcctgg tgctcgacat gcgtgctaac
      361 cccggcggtc gcatggagca agccatggat atcgcccaca tgctgctggt ggaaggtccc
      421 accatcacca ctctgacgcg ggacggcgat tccattgccg aactgcgcga ggtgctcttc
      481 aatgatcaca atgcccgcat caccgttaag aacggagaga ttctgcaaag caagaatgaa
      541 ccgcgggttt tccggctctt tcctgataac agaccgatcg tggtgctggt tgacggcaac
      601 agtgccagcg cctctgaaat tctggccgga cttctgcaac ggaatggtcg ggccctgatt
      661 gtgggagagc ccagcttcgg caaaaccgtt ggtcaaaacg ttgtgccgct tgtcttcggc
      721 accggcttga aaatcaccac cttcagcttt ctgcctggtg gtgctgaact tggcgtggcc
      781 gtgcttccgg atcgggaagt ggcagccagt gatgccttta aagacgatcc gattcaggcc
      841 gaggatgtgc agctgacggc agccgtcaat gctattgcgg agttaaacgg caaggttgag
      901 gcactggcca agctgaaagc acgtgttcag gaggaacaca gccgcagaga cagtcgcatg
      961 ctggacgctc aaggccgcgg tcttgagctg attccggtgc cggctaagta gaacaaccca
     1021 aacctcacaa acccttccaa caggaaaaac taatgaaagc aatcttcgat ccgatcttcc
     1081 tcttcgccct cactgttgcg gctgcttatt gcggctactt tgtcaacaaa gtgcggggct
     1141 ggttcactac tcgtggtgcc ctggccgtga cggtctttgg cgcagtgatg tacctggggc
     1201 tcattttcgg aatgggcttt ggcaatgaag gcggactcta tgtgctgtcc tttggcttcg
     1261 gcgccttgct gtcttgcatg gcgccacgct atgtggccat tatcttgaag cccttcgccg
     1321 tcaccttcgg acttttgcgc cgctttgtgg cgctggtttc gaaaccggtg cgggcacgtg
     1381 ccgatagcag tgatgccact catccaacct cgtccttcct gctggttttc ttgcacaccg
     1441 ctgaaacctt cttcggtgat atggagaatc ttttcggtaa gtatgccgaa cgtcatccgg
     1501 cggccagccc tcttcaaccg ggtgttggag ccaagtaagg cttgcctgag atagaccggc
     1561 ttccaggtca tcgctgaccc gttcttatgc cgggaggaga caggagtaag aaagtgttct
     1621 cccggcgatt tcttccgaat gccaaaaaaa acgaaaaaac aggaatactc atgaaagaaa
     1681 tcgacaaaac aaccctcggc gcaatcttac acgcggcagt caagctgcgt tcccatgtgc
     1741 cgaattcaac catagtttcg gcgtgcctgg acagtcatg
//
LOCUS       DAOQNZ010000960         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48238_length_1779_cov_4.352088, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000960 DAOQNZ010000000
VERSION     DAOQNZ010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48238_length_1779_cov_4.352088"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKW73_15305"
     CDS             complement(<1..446)
                     /locus_tag="PKW73_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74714.1"
                     /translation="MKFRHNALKEILRNKFGDSIAEFGPALFVILMFLFFPMLNMVSI
                     GVSYGLGMVLNYNQVHEAALLPSADALSSSGPIKKSIPEQWQAGMGQFAKLSGSPVTN
                     IAYRDGTTGLGSGAGQSDTTDKIVKVQTTITCNPFLPIPLPIANIPG"
     gene            695..1435
                     /locus_tag="PKW73_15310"
     CDS             695..1435
                     /locus_tag="PKW73_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74715.1"
                     /translation="MTFGEIRHLAGKRWAFSLPREHGATVSLSLASFTALGLSSGTSS
                     AVFSDAVLLVSTSLLMLWAMLLSMASSRQLLVVSLISSALLYCLGGLPFALWGAFCGL
                     GLSLFGAVAPVRNSLWWRELFGLTGSVVCPLMFSYLARPDLVLHLIAAGALLASIFSG
                     SALIRLSRKDLGLSPLLPGALSFLCWLILATLSAETAALALLPYVLQSICLLQVDQTE
                     KPSLKVLGKTQAFSLLFVVAVLVLRILA"
     gene            complement(1515..>1779)
                     /locus_tag="PKW73_15315"
     CDS             complement(1515..>1779)
                     /locus_tag="PKW73_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74716.1"
                     /translation="PGENETDFVRKGGAHKETVERKCLCNALLATVGLAQRRADYIEP
                     PIITAGDHALELKPFVEREGKQYSARAVVRHILQKPQLLQEST"
BASE COUNT          309 a          444 c          496 g          530 t
ORIGIN      
        1 ccggggatat tggcgatggg caaaggaatg ggcaggaagg gattgcacgt gattgtggtc
       61 tgaactttca cgattttgtc ggtggtgtcg ctctgacctg ccccgctgcc cagtccggtg
      121 gtgccgtccc ggtaggctat attggtgaca ggactgccgc tcagtttggc gaactgtccc
      181 ataccggcct gccattgttc gggaatgctt ttcttgatcg ggccggaact gctcaaagca
      241 tcagcagacg gcaagagggc cgcttcgtgt acctggttgt agttgagaac catgcccagt
      301 ccgtaagaga cgccgatgct caccatgttc agcatgggga aaaagagaaa catcagtatc
      361 acgaaaagag ccgggccgaa ttctgcgatt gagtcaccga acttatttct cagaatttct
      421 ttgagggcgt tgtgtctgaa tttcatggct gcctcccaga ggcgcagcgc ctctttagca
      481 agctgagaat tggcccgttc taattttaag tcttttataa aacgggggta ttctcctgaa
      541 gactggttct tttgccgatt catccctgtt gctgatcagt ctgccttccg gcacggtctt
      601 tggcggcaaa tccggttaca ttttttagtc ggcctttcgg aggattatta gtaaaccgca
      661 gtccgctagt atcaaacaga tggggaggtg tgccatgacc ttcggagaga ttcgacattt
      721 agccgggaag cgttgggcat ttagcttgcc gcgagagcat ggcgccacag tctctctttc
      781 tcttgccagc tttacggctc tggggctaag cagcggcacc tcctccgctg ttttctcaga
      841 tgccgtattg ctggtttcta cgtctctgct gatgctctgg gccatgcttt tgtccatggc
      901 ctcttcccgg cagcttcttg tggtttcgct tatctcttcg gctttgcttt actgcctggg
      961 gggactgcct tttgccctct ggggagcctt ctgtggtctt ggtttgagtt tatttggagc
     1021 ggtagcaccg gttcgcaaca gtttgtggtg gcgtgaattg tttggcctga ccgggtcggt
     1081 agtttgcccg ctcatgttct cctacctggc caggcctgat cttgtcttgc atttgattgc
     1141 tgcgggcgct ctgcttgctt ccatcttcag cggctcggcc ttgattcgat tgagccgaaa
     1201 agacctgggt ttgagtcctc tactgcccgg tgcgctctct tttctgtgct ggttgatact
     1261 ggccactttg agtgcagaaa cggccgctct ggcgctttta ccctatgtgt tgcaaagtat
     1321 ttgtctgctc caggtcgatc agacagaaaa accatccctg aaagtactgg gcaagaccca
     1381 ggcttttagc ttgcttttcg tggttgctgt gttggtcttg cgtattttgg cttgagcggc
     1441 tactgcgccg gtcttaccaa tttttccgta acggctgatg tttatttgcg gctattagat
     1501 tgagcggcag tcggctaagt gctctcttgg agaagttgag gcttttgcag aatgtgccgc
     1561 accactgctc gggcggagta ttgtttgcct tctcgttcta caaaaggctt cagctccaga
     1621 gcgtgatcgc cggctgtgat aatcggcggc tctatatagt ctgcgcgcct ttgagccaga
     1681 ccgacagtgg ctagaagagc gttgcagaga cactttcgct caacagtttc tttgtgggcg
     1741 ccgccttttc tgacaaaatc tgtttcattt tctcccggg
//
LOCUS       DAOQNZ010000961         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48323_length_1778_cov_2.687174, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000961 DAOQNZ010000000
VERSION     DAOQNZ010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48323_length_1778_cov_2.687174"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PKW73_15320"
     CDS             <1..1396
                     /locus_tag="PKW73_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74717.1"
                     /translation="FFAESDESKLSQALRSVLSLDGWYVRMQESGEPLYLEQVPEQFK
                     FFPYTDLSGLAKAESKGGKSKESSPKYRPVKEAGVSYLVLHRQECHSTTPIPAREIVK
                     YLPAKCGGILFYMEAEDAGTKPLFLPEKYIERLKSLTEAMQVEHYLVFPGGAGAQLET
                     LLKHQWQVEVRGDEIVKSTIFSDLKMVLAATHRDKCPFSETRAMSGQDLFAWVAQQDD
                     VDGLAVNVVGKLESGGRPVNNLVMAPGIVHALSRGNDIRAGAAPLPARSLKEVEHYLK
                     QVDFPGKDRRFVEAVVKDDSGQEVVLLRAVVEGASTWRIQDSQGGQLLAHEEGLTWSP
                     VFQLPLNYKQVKGFGEGKTKILCAGHLATELGARSYRAEDINYLWRPGRFLLLGRLLD
                     EQDRRSSQHRMEVAGELLKLLDEQDRIPRSAILTVEGAKTFSLKPFTRGKQWLEASLK
                     RAEQFTEAFAWGVA"
     gene            complement(1444..1695)
                     /locus_tag="PKW73_15325"
     CDS             complement(1444..1695)
                     /locus_tag="PKW73_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74718.1"
                     /translation="MLVNSVLLRRSDDAEIELFDERDSFIASFKNGKWVKALVFSDYE
                     LEDFDLIEDEREIERVLAEARAALGCPLKELSDDRTKSA"
BASE COUNT          457 a          461 c          470 g          390 t
ORIGIN      
        1 cttcttcgcc gaaagcgacg aaagcaaatt atcccaggca ttgagatcgg tattatccct
       61 ggatggatgg tacgtgagaa tgcaagaaag tggcgagcct ttatatcttg aacaagtacc
      121 ggagcagttt aagttctttc cctacaccga cctgagcggt ctggctaagg cggagtcaaa
      181 aggcggcaag agcaaggaga gtagccccaa gtaccgaccg gtaaaggaag cgggagtctc
      241 ttacctggtc ttgcataggc aagagtgcca ttcaaccaca cccatacccg cccgcgaaat
      301 agtcaaatac ctgccggcaa agtgcggcgg tatcttattt tatatggagg cggaagatgc
      361 aggaacgaaa ccactcttct tgccggagaa gtacatcgag cgcctcaaga gcctgactga
      421 agcgatgcaa gtagagcact acctggtctt tcccggtggt gcaggagcac agttagagac
      481 cctgctcaaa caccagtggc aggtggaagt gaggggcgat gaaatagtta aaagcactat
      541 tttctcagat ctcaaaatgg tgttggcggc cactcacaga gacaaatgcc ctttcagcga
      601 aacccgggcc atgagcggcc aggacttgtt tgcctgggtg gcacagcaag atgatgtgga
      661 cggactggcc gtaaatgtag tcggcaagct ggaaagtggc ggcagaccgg tgaacaatct
      721 ggtcatggcc ccggggatag ttcatgcact gtcacgcggt aatgacatac gagcaggagc
      781 agccccgctg ccggcccgct ccttgaaaga agtggaacat tacttgaagc aggtcgactt
      841 ccccggcaaa gacaggcgct ttgtggaagc cgttgtaaaa gacgacagcg gtcaggaagt
      901 ggtgctcttg agggccgtgg tggaaggggc cagcacctgg agaatacagg acagccaggg
      961 agggcaactg ctcgcccacg aagagggtct gacctggagc ccggtcttcc aattgcccct
     1021 caactacaag caggtgaagg gctttggcga gggcaagact aagatacttt gcgccgggca
     1081 ccttgccacc gaacttggtg cacgttcgta ccgggctgaa gatatcaatt acctctggcg
     1141 tcccggccga tttctcttat tgggtcgact gctcgacgaa caagacaggc gaagcagcca
     1201 acatcgcatg gaagtagccg gagagttatt gaagttgctc gatgagcaag accgcattcc
     1261 tcgctcagcc attctcacgg tggaaggggc aaaaactttc agtcttaaac cattcaccag
     1321 agggaaacag tggctggaag ccagtctcaa gcgcgccgaa caatttaccg aagcttttgc
     1381 ctggggtgtg gcttaagcgc tttgaactgc ggcaccgaaa ggtaaagcca agcactcccg
     1441 ccttcacgcg ctcttcgtcc tatcgtccga taactctttc aaaggacagc ccagcgcggc
     1501 tctagcctcc gccaaaactc tttctatttc ccgctcgtcc tcaatcagat caaaatcttc
     1561 cagttcataa tcgctgaata ccaaggcttt tacccactta ccattcttaa acgaagcaat
     1621 gaagctatcg cgttcatcaa aaagttctat ctctgcatca tcagaacgcc ttaataatac
     1681 cgagtttacc aacatttgct caccgtcctt tggcagtctc tattgtcttt tcgatcaatt
     1741 ttattattgt ttcttgcgtt tgccctgtcc gattaagg
//
LOCUS       DAOQNZ010000962         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48470_length_1775_cov_3.100000, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000962 DAOQNZ010000000
VERSION     DAOQNZ010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48470_length_1775_cov_3.100000"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            568..>1775
                     /locus_tag="PKW73_15330"
     CDS             568..>1775
                     /locus_tag="PKW73_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74719.1"
                     /translation="MKFPNLFTNFFPSAKAFVGGLRYRPVMAFFVLVFLIVAYCVVMP
                     FIQPAVFTMFRYLPLVLSLLVSGFLGYVYFRKIGSRGGFINAMVLTFLLALPGWMAIA
                     NFADVMQEWSIALTLEPRALTKPPYSTDRRSLNKATASIYAQNSNHESLLTSGHPHIA
                     ADMSDSEHPRMWWQVPFYYREGLNYYLGSVAKIVRIDADSPNMAVDLKAGEKAEFYFG
                     EESTYVHALFALRLPFSRPSHAQYYRHADGTWSLLIPAISYRPTITGAMIPEYTKVMQ
                     VLPSGNYHVHSAADAGKLFPGAPLFPLELGRLYSASYGKFKSGLINYYDTVLGRNNIF
                     EISEDDVAAGHVLDESQNATDNRFPFYQTFLGEGLKQMVPLEPVGPSFALSEILLFDA
                     GTGEASSYIPA"
BASE COUNT          363 a          469 c          453 g          490 t
ORIGIN      
        1 atggcttctc gcctcgagaa gtttattgcc gctttgaagg ggtagttcat ccttaaaacc
       61 gccgggagaa ttggcggcgg tgggccgctt agggttctct cggcgggacg gtgctaggcc
      121 gtcccggcct gagatccgct cgcgcggttg gatttttgca gtagcaagac ggcaagttaa
      181 cgcttgcagg ctatttcttg ggacagtggt acgatcgcga tcccgggaag atatttttga
      241 acctgaagta ctcagcttct taacaggttt tttctaaata ctcattgtca ttgcttagtt
      301 gatttgtaca tagtgactat gtacttcctt tttcaactta gctctggagc tttaaaaaag
      361 ccgctaattc tcaactatag ctcttatctc tattggctcc cgctttcagt tcgaagccgt
      421 ggatacatac cctggtcacg ggttgtccga acaagagcta agttaccggc agtacaaacc
      481 tctgttcgcc tttggaaaac gtttcgaagg cgccttttaa tgggtcccga cactgagtcg
      541 gtgccctaaa ttcgtggagt aacaaccgtg aaatttccaa acttgtttac taatttcttt
      601 ccttccgcaa aagcattcgt cggcggcttg agatatcgcc ccgtcatggc cttctttgtg
      661 ctggtgttcc tgattgtggc ttactgcgtg gtaatgccct tcatacagcc ggctgtgttt
      721 accatgtttc ggtatttgcc cctggtgctg tcgcttctgg tgagcggatt tctgggttac
      781 gtttattttc gcaagatcgg gtctcggggc ggttttataa acgccatggt gcttactttc
      841 ttgctggcat tgccgggctg gatggctatt gccaacttcg ccgatgtgat gcaggaatgg
      901 agcattgctc tcaccctgga accgcgtgcg ctgaccaagc cgccttacag tacggaccgg
      961 cgttcgctca ataaagcgac cgcttccatc tatgcgcaga actccaatca tgagagcttg
     1021 ctgaccagcg ggcaccctca tattgccgcc gatatgagcg actccgaaca ccctcgtatg
     1081 tggtggcaag tgcctttcta ttaccgggaa ggactcaact attacctggg ctcagtggct
     1141 aagattgttc gtatcgatgc tgatagccct aacatggccg tggacttaaa agccggcgag
     1201 aaagcggaat tctacttcgg ggaagagtcg acttatgtgc atgctctctt tgccttgcgt
     1261 ctgcctttct caagacccag ccatgcgcag tattacagac acgccgacgg cacctggagc
     1321 ttgctcattc cggccatctc ctacagaccg accatcaccg gcgctatgat tcctgagtac
     1381 accaaggtca tgcaggtctt gccgagcggt aactaccacg tacacagcgc tgccgatgcc
     1441 ggcaagttgt tccccggtgc gccgcttttc cctttggaat tgggtcgcct ttactcagcc
     1501 agctacggta agttcaaaag tggtttgatt aactactacg acacggttct gggtcgcaac
     1561 aacatctttg aaatcagcga agatgatgtg gctgccggtc atgtgttaga cgagtcgcaa
     1621 aacgccaccg acaaccgttt ccccttctac caaactttcc tgggcgaagg tttgaaacaa
     1681 atggtgcctc tggagccggt tggtccgagc ttcgcgctgt ctgaaatttt gctgttcgat
     1741 gccggcaccg gcgaggcctc ctcttacatt ccggc
//
LOCUS       DAOQNZ010000963         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48705_length_1771_cov_2.669580, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000963 DAOQNZ010000000
VERSION     DAOQNZ010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48705_length_1771_cov_2.669580"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1090)
                     /locus_tag="PKW73_15335"
     CDS             complement(161..1090)
                     /locus_tag="PKW73_15335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA74720.1"
                     /translation="MALAFLAVYLIWGSTYIAIKFALETMPPCFLGAVRFTGAGLLMC
                     LLAVLRKEPRPTLEQLRSAAVLGMLLLVFGSMGVVFAERTVPSGLVSLLVTSVPIYIV
                     LLQWILPGSMGRAPSLKVLLGLAFGVMGLALLLDLDTIFGAEKIDLAGAAWVLIGAFG
                     SACGALYTRVARVHQSKIYSVGLQMTFAGLFLLLASFATGEWSHIDLQGITAKSLWSL
                     LYLAVFGSIIGFSAYVYLLQNVSPSRVATYAYVNPVVAVLLGWLLGGEAVTTRTMISA
                     VVVIAAVWMINTGQHKSFLAPRSEKKEEEDGAA"
     gene            1278..1757
                     /locus_tag="PKW73_15340"
     CDS             1278..1757
                     /locus_tag="PKW73_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNA74721.1"
                     /translation="MSSKQASTLSVRSLLASLITFVCIGIGAGQAQAKWPFMGGQARG
                     EEGEVRAKSLYIKEINDRKFNNQVLKSEAPVVVTFYSDMCEACRALDPIMDRIAGSYQ
                     GKVKFYRILVDENPRSSQRFNIKKVPTVAVFKGGKLMGGSVGYTPGETLKERIDQAL"
BASE COUNT          503 a          485 c          453 g          330 t
ORIGIN      
        1 ggagggccga ccaccgcgct caataagaac ccggtgcaaa gacttgcctt aagactgcgc
       61 gacaatttca cttatgacaa ctccgaattt accaattacc ttaccactga acaggtaaat
      121 ggatttagga gcactagttt tacacttaaa gcagtctttc tcaagcggcc ccatcttctt
      181 cctccttctt ctccgaacgg ggcgccagga aggacttgtg ctgcccggtg ttgatcatcc
      241 agacggcggc aatgaccaca accgcagata tcatggttcg ggtggtgacg gcttcgccgc
      301 ccagaagcca acccaggagc acagccacta cgggattgac ataagcatag gtagccaccc
      361 gagatggtga gacattctgc aaaagataga cgtaggccga aaacccgatg atcgatccaa
      421 aaaccgcaag atatagtaag gaccagagcg actttgcagt tatgccctgc agatcgatat
      481 gagaccactc tccggtggca aaagatgcaa gcagaagaaa caacccggca aaggtcatct
      541 gcaaaccaac cgagtaaatc ttgctttgat gaaccctggc cacgcgagta taaagggcac
      601 cgcaagcgga gccgaaggcg ccaatcagca cccaggccgc accggctaga tcaattttct
      661 ccgcaccaaa tatagtatcc aggtcgagta acaaggccag acccatcacg ccaaaagcaa
      721 gaccgagaag cactttcaag ctgggagccc gccccatact gcccggcaaa atccactgta
      781 acagcactat gtaaatgggc acggaggtga ccaggagcga gaccagcccc gagggcacgg
      841 tgcgttcggc aaaaaccaca cccatactgc cgaagaccag caaaagcatg ccaagtacgg
      901 cggccgaccg cagttgctca agggtaggtc ggggctcctt gcgtaagacg gccagcagac
      961 acatgagcaa gccggcacca gtaaaccgca ccgcccccag aaagcagggc ggcatggttt
     1021 caagggcaaa cttgatggcg atataagtgg acccccagat aagataaacg gccagaaagg
     1081 caagcgctat cttcaggggg cttggctcac gttctctggt atctgcctga aaaatgcctg
     1141 caccgggggg atgtaccagt gaagattaca acgcttaagc cgatattatc aagtgtttca
     1201 ttaacagaat caccaactcc atgaaaggca gaatcgctct ttatggctca tactgactga
     1261 agtgtggagg tatatcaatg agttcaaaac aagcgtcgac actatccgta cgctcccttc
     1321 tggcaagctt aatcactttt gtttgcattg gcatcggcgc cggtcaggcc caggcgaagt
     1381 ggcccttcat gggtggacaa gctcgcggtg aagagggaga ggtccgcgcc aaatccctgt
     1441 atataaaaga aatcaatgac cgaaaattca acaaccaggt attgaagtct gaggcaccgg
     1501 tagtagtaac tttttactcc gatatgtgcg aagcctgccg ggccttagac ccgattatgg
     1561 accgcatcgc cggcagctat caggggaaag tcaagtttta tcgcatcctg gtggacgaga
     1621 atccccgctc ttcacagcgc ttcaatatca agaaagtacc caccgtggct gtcttcaaag
     1681 gcggcaagtt gatgggcggc agcgtcggtt acacccccgg cgagaccctg aaagagcgca
     1741 tcgaccaggc cctttaaaca aaaacaaggg c
//
LOCUS       DAOQNZ010000964         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48750_length_1770_cov_3.495044, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000964 DAOQNZ010000000
VERSION     DAOQNZ010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48750_length_1770_cov_3.495044"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /gene="fusA"
                     /locus_tag="PKW73_15345"
     CDS             <1..429
                     /gene="fusA"
                     /locus_tag="PKW73_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNA74722.1"
                     /translation="GVLAGYPVIDVKVSLVFGSYHDVDSNEMAFRTAASMGFKEGFMK
                     AQPTLLEPVMKVEVEVPEEFMGDVIGDISSRRGRVEGMETISGLSKVRAQVPLSEMFG
                     YATDIRNKTRGQGTFTMEFAQYEEVPSNIAEAVITSKKEK"
     gene            533..943
                     /locus_tag="PKW73_15350"
     CDS             533..943
                     /locus_tag="PKW73_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74723.1"
                     /translation="MKYLHSGYILFCQEASHEENGRVDARGLFDLFVVKEFPAKMDCM
                     CVLGFGTPYERRQYKGTVTIEDPDGNIIFEKEFHANDPADIFKGHYLFKAEATLPKEG
                     AYAAKISLKNWKDDTMWDFERKFFSMLEGSPVDP"
     gene            complement(947..>1770)
                     /locus_tag="PKW73_15355"
     CDS             complement(947..>1770)
                     /locus_tag="PKW73_15355"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNA74724.1"
                     /translation="LGRYLDWQDKSDLASRMLAAVNIDPVLPKSLLARKVTVPSVYDL
                     GDFRFKVDSHYQDDRERQLSCFRDIYKDYQFKGALAGQLVKTGLAASAASEKLHKLTA
                     DAAAKSSAAYPDGRLSASLRLIAQMVAGQVGARVYTLQMDGFDTHANQSRTHNSLLGN
                     LSRSLKAFLDDLESRGVADEVLVLVHSEFGRRAQENDGRGTDHGAAAPCFVLGKGVKG
                     GLYGEHPELAANKLVQGDLGFKTDFRSVYSALLEDWLGSDARAILGRSFEKIPFV"
BASE COUNT          444 a          482 c          461 g          383 t
ORIGIN      
        1 ggcgtactgg ctggttaccc ggtcatcgac gtgaaagtat ccctggtttt cggttcgtat
       61 cacgatgtgg actccaacga aatggctttc cgcaccgctg cttccatggg cttcaaagag
      121 ggcttcatga aagctcagcc caccctgctt gaacctgtta tgaaggttga agtagaagta
      181 ccggaagagt tcatgggcga cgtcatcggc gacatctcca gccgcagagg acgcgttgaa
      241 ggtatggaaa ccatcagcgg actatccaaa gtaagagccc aggttcccct ttctgaaatg
      301 ttcggttatg ccaccgacat cagaaacaaa acccgcggtc agggcacatt caccatggaa
      361 ttcgcccagt acgaggaagt tccctccaac atcgccgaag cagttatcac ttccaaaaaa
      421 gagaagtaat aacttcaaag aaagatcaaa gagaagcccg ggtacccccc gggtttcttt
      481 tttgctgtta atatgtacgc tctcgcatct gtattcaggt ttggaagtag tcatgaagta
      541 tctacatagc ggatacattt tgttctgcca ggaagccagc cacgaagaaa acggccgtgt
      601 cgacgcacgt ggactctttg atttattcgt tgtcaaagag ttccctgcca agatggactg
      661 catgtgcgtg ctgggcttcg gcacgcccta tgaaaggcgg cagtacaaag gtaccgtcac
      721 catcgaagat ccggacggca acataatttt cgagaaggaa ttccacgcca acgacccggc
      781 tgatatcttc aaaggacact accttttcaa agcggaagcg accctgccca aagaaggtgc
      841 ctacgccgcc aagatttcct tgaagaactg gaaagacgat accatgtggg acttcgagcg
      901 caagtttttc tcgatgctgg aaggcagccc ggtagatccc tgatctttag acaaaaggga
      961 ttttctcgaa actccggccc aggatggcac gggcatcgct gcccaaccag tcctcaagca
     1021 gggcagagta aactgagcgg aaatcggttt taaagcccag gtcaccctgc acgagtttat
     1081 ttgccgctag ctcgggatgc tcgccataaa gaccgccttt aacgccctta cccaagacaa
     1141 aacaaggggc ggccgcaccg tgatcagtgc cgcgtccgtc gttctcctgg gcccggcgac
     1201 cgaattcgga atgaaccagc actaaaacct cgtcggccac gccgcggctt tccagatcgt
     1261 cgagaaaggc cttgagcgag cgcgacaaat tgccgagcag actgttatgg gtgcggctct
     1321 gattggcatg ggtatcaaag ccgtccattt gcagagtgta cactctggcc cccacctgtc
     1381 ctgccaccat ctgcgctatc aaacgcaaag aggcggagag ccggccatcg ggataggcag
     1441 cgctgctttt tgcggcggca tccgctgtca gcttatgcaa tttctctgaa gcggcactgg
     1501 cagccagacc ggtcttcacc agttgaccgg cgagagcccc cttaaactga taatctttgt
     1561 agatgtctct gaaacaactg agctgccttt ctctgtcgtc ttgataatgg gaatctactt
     1621 tgaaacgaaa atcacccagg tcgtagacgg agggcacggt taccttgcgg gccagaagcg
     1681 acttgggcaa cacaggatcg atgttcacag cggcaagcat acggctggca agatcgctct
     1741 tatcttgcca gtcgaggtag cgacccagcc
//
LOCUS       DAOQNZ010000965         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48920_length_1767_cov_5.799650, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000965 DAOQNZ010000000
VERSION     DAOQNZ010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48920_length_1767_cov_5.799650"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1371
                     /locus_tag="PKW73_15360"
     CDS             226..1371
                     /locus_tag="PKW73_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74725.1"
                     /translation="MAKHAGPALIMAALTIAFHPGAAVGQSADSGEAYGPLLSPQRPG
                     SERGVRLSRPQSQSAKPERSAEVPPLAQATCKERVEILTSELVWYKSLARARSEAERS
                     GKPIFWVHMLGQIDGNTCGAASALRAVSLAKDPVYSILKDDFVVGYKNIDGKNFAGIP
                     HQHGPDENVGDTTNGAGAHNLQLLVLAPDGTVLTCLPGYWSGPDLAFELAFAKDLYKL
                     WKDADLTVDEKRARYSSLHLAHISTHSQAMKNRSHLSEADLQFEGRNLENSDFFYQQR
                     AVNPSTGKTPLRNVKTIDIIMHERLGGQPFALYQDFDAAAFADFGRPLSLLVQGTAAE
                     KPSLSNQASSNQNKGSAVKSAFARTGKSLGRTTVNHAIRYGIRSILP"
     gene            complement(1402..>1767)
                     /locus_tag="PKW73_15365"
     CDS             complement(1402..>1767)
                     /locus_tag="PKW73_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74726.1"
                     /translation="TDGLEVLEISEIEALLTKAIRPSQAASSLIQAVKDKQTNIIDAQ
                     DNTTVVVIAPVVDPIIASPAMTSKPGAAPQVCDSLSQNFSKMVTTQSSSCPEQSENQQ
                     MGFRGWPLYSAQDTVKSWF"
BASE COUNT          389 a          456 c          480 g          442 t
ORIGIN      
        1 cgatgccaag gattatttcc atggaggtga gagaggccag tgccgcccag acttcaccat
       61 ccaatagtaa atttgtatcc atatccttta tgacagaccc ttcttttgat tgccccaagg
      121 cttcaatagg gcagacagcc ctttgagcag gttctaagta tagatgtttt ggtttgcttt
      181 atagcaagag tttgttagga tgtactggtc gatggagatt tgcccatggc aaagcacgcc
      241 ggtccggctc taattatggc cgctctgacc atagcttttc atcccggtgc tgccgttggg
      301 cagtctgccg atagcggtga agcttacgga ccattgctca gtccacagag acccggttct
      361 gaacgcggcg ttcgcctgag ccggcctcag tctcaatcgg ctaaaccgga gcgtagcgct
      421 gaggtgccgc ccctggcaca ggctacctgc aaagagcgag ttgaaatact gaccagcgaa
      481 ttggtctggt acaagtcgct ggcccgggcc aggtcagagg ccgagcgcag cggcaaacca
      541 atattctggg tgcatatgct ggggcagata gacggcaata cctgcggggc ggccagtgcc
      601 ctgagagctg tctctcttgc caaagatcct gtctattcca ttttgaaaga cgactttgtg
      661 gtgggctaca aaaatatcga cggcaagaat ttcgccggta ttcctcatca gcacggcccc
      721 gatgaaaacg tcggtgatac tactaatggt gctggtgccc ataacttgca gctcttggtg
      781 ctggcccccg acggcacggt ccttacctgt ttgcctggtt actggagcgg gcccgacctg
      841 gcttttgagt tggcttttgc taaagacctt tataagttat ggaaggatgc ggacctcacc
      901 gttgatgaga aacgtgcccg ctattcttct ctccatctgg ctcatatttc cacccacagt
      961 caggctatga aaaatcgcag tcacttgtct gaggctgatc tgcaattcga gggtcgaaat
     1021 ctggagaatt ccgatttttt ctaccagcag cgcgccgtca atcccagcac cggtaagaca
     1081 ccgttgcgaa atgtcaaaac catcgacatt attatgcatg aaaggcttgg cgggcagccc
     1141 tttgcactct accaggattt tgatgcggcg gcctttgctg atttcggtcg acctctgtca
     1201 cttctagtgc aggggactgc cgctgaaaaa ccttccttaa gcaatcaagc ctcaagcaat
     1261 caaaacaaag gcagtgccgt caaatcggcc ttcgcaagaa ccggtaaaag cctgggaagg
     1321 accacggtca atcatgccat tcgttacggc atacggtcta ttttgccctg atggtgcttt
     1381 cggcagctct gcaaatgtga ctcagaacca cgactttact gtatcctggg cgctatacag
     1441 aggccagccg cggaagccca tttgttgatt ttccgattgc tccgggcacg agctgctctg
     1501 agttgttacc atcttggaga agttctgaga gaggctgtcg cagacctgag gagcggcgcc
     1561 gggtttggag gtcatggccg ggctggcgat aatcgggtcc accaccggag cgatcactac
     1621 aaccgtggtg ttatcctggg catcgatgat attggtttgt ttgtctttga cggcctgaat
     1681 gagactgctg gcggcctggc tggggcgaat ggctttagta agtagggctt cgatttctga
     1741 aatttccaga acttccagac cgtcggt
//
LOCUS       DAOQNZ010000966         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48943_length_1767_cov_3.450350, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000966 DAOQNZ010000000
VERSION     DAOQNZ010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48943_length_1767_cov_3.450350"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..1099)
                     /locus_tag="PKW73_15370"
     CDS             complement(332..1099)
                     /locus_tag="PKW73_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA74727.1"
                     /translation="MVLEQAAKLIPRIAPEVSEVSTTLLAETIPAIQSGIKTYVFGGA
                     KSNAGNAGKLASIHFPKNGPIWNEEVLFRHSQQVELGEELLFGDATAAELKQAKTSAQ
                     WLENAGFEFNSFKPYFTNKRLATGEGTMSPNVMRRLTEGSQNTMEGSTSALSRHYFRD
                     NTGQAHLMDIYDTKGAAERLGYARRTVQELAYTGKVPAHMVKGDHGLEWRILLPAEKV
                     TAQVASKPTTQMTEELLFRKAANGDPSKDFFRMPMWR"
     gene            1455..>1767
                     /locus_tag="PKW73_15375"
     CDS             1455..>1767
                     /locus_tag="PKW73_15375"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74728.1"
                     /translation="MIACYLTLLPVAAATNLEEGIKLYNEKQYSNARLLLEKAVTESP
                     TSWQAHYYLANTCLVLGQMTKAKFHYTMCQKNCAYGEILAHCQTGIARTEKHLPKAAS
                     SS"
BASE COUNT          380 a          408 c          467 g          512 t
ORIGIN      
        1 gattctgctg ccgacaatta gcggaattag gtgccgggag tttccggaat ttacacaagt
       61 caaaagttga agctttggtt cgcttctgga ggctatcttc ggcttactgt atcaggttat
      121 ttttgctctc ttggaccggt cgatcatagc ggtagaaaac ttgcgctacc agtgttcttg
      181 aacagaaagt atctttgtaa tacctaatgc cagcgagggt tacagaaagg tcgttcgtca
      241 gggtctcgta ttggcaggtt gagagaaggt cgtgtcagcc tttactgctg tatgccgaca
      301 cctgcgataa gaccgtagcg acacgctagt actatctcca catcggcatg cggaagaaat
      361 ctttagacgg atcaccgttg gcggccttgc ggaagagcag ttcttccgtc atttgtgtgg
      421 tcggtttgct tgcgacttgg gcggtgacct tttcagctgg cagtagaata cgccattcca
      481 gaccatggtc gcctttcacc atgtgagccg gtaccttgcc tgtgtatgcc agttcctgaa
      541 cggtacgtct ggcgtagccg agtctctctg ccgcaccttt ggtgtcataa atgtccatca
      601 aatgcgcctg tccagtgtta tcacggaagt agtgtctgga tagggccgaa gtgctgcctt
      661 ccatagtgtt ttgagagcct tcggtcagcc ttctcatcac attgggtgac atggttccct
      721 cgccggtcgc aaggcgctta ttggtgaagt acggtttgaa tgagttgaat tcgaatccgg
      781 cgttttccag ccactgagca cttgtctttg cctgtttaag ctcggctgct gttgcatctc
      841 caaatagaag ttcttctccc agttccactt gctggctgtg acggaagagc acttcctcgt
      901 tccagatcgg gccgtttttg gggaagtgta tactggcaag ttttcctgcg ttgccggcgt
      961 tggacttcgc tccaccgaac acataggtct ttatgcctga ttgtatggct ggtattgttt
     1021 cggctaacaa cgttgttgac acttcgctta cttcaggggc aattctggga attagtttgg
     1081 ctgcttgctc aagaaccatt tgactttcct ctggggcgtt gccttaatgt agcctgcggg
     1141 tgtgtccgag gtgtggcgcc tgatgggggt aaatacgaag tcttgctcct ccggatctaa
     1201 tagtggcagc gcagtctagt aagcgccagt tgcaggcgca gaattggaat tgtggaagat
     1261 gtatttactt tccttgcagg gatgggcggc caacagattg aggcatttta accgtaatgt
     1321 ttatggctcg aatcttgcca tcggtgtata actgattaag gtgacacagt cattgatagt
     1381 ctctcagttg ggcgctgcag gtagtttgaa gtaaatttat gcggttaagg cttttaagtt
     1441 ccagtttact gctgatgata gcttgctatc tgacgctttt gccggttgct gccgccacaa
     1501 atcttgaaga aggtatcaag ctatacaatg aaaaacaata ttcgaatgcc cggctgctct
     1561 tagagaaggc ggtaacagag tcacccacgt cctggcaagc ccattactat ctggctaata
     1621 cctgtcttgt gcttggacag atgaccaagg ccaaatttca ctatacgatg tgccagaaaa
     1681 attgtgcgta tggagagatt ctcgctcatt gccaaactgg tatagcccgc acggagaagc
     1741 acctgcctaa agccgcctcg tcctcta
//
LOCUS       DAOQNZ010000967         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48944_length_1767_cov_3.446262, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000967 DAOQNZ010000000
VERSION     DAOQNZ010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48944_length_1767_cov_3.446262"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PKW73_15380"
     CDS             complement(<1..1055)
                     /locus_tag="PKW73_15380"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA74729.1"
                     /translation="MKYFSGAQTYQILDVEAVSVQAAAADGALKSFTSPQAQEVPSLA
                     ADIAIIGGGMGGVAAALTALTANSGGTVIMTEETSWLGGQMTSQGVSALDENKWVENT
                     GACLSYLQLRDKLRESYRQTGGLTPEAAQDPLLHPGRSWVTRLSFEPARALAVIDQTL
                     APYIAAGRLTILTRSQIVDAHCREDNRIEALLAVNVENGQFTEICPKTVLDATELGDL
                     FPLVKVPYHTGSDSKRLTNESHAPEQGDSENVQDFTYPFVLTLHAGEDHTIEKPDDYE
                     TFKDAGKFSLFGFKMFEHYEGFDPARPENVKNFLPFWTYRRLIDKSLFDGSVYKFDIS
                     MINWDANDLRGYNIIDK"
     gene            1148..1741
                     /locus_tag="PKW73_15385"
     CDS             1148..1741
                     /locus_tag="PKW73_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNA74730.1"
                     /translation="MAAGRKVVESRSVDLSLLSDEVKKRASRVKLVLMDVDGVLTDGT
                     LYYFPGPDGKPTEFKGFNSQDGAGLHFLNLCGIKTGVISGRDSQAVNERARILKITYI
                     YQGYLEKESIYEEILSKEGVDDDAVCFIGDDFPDYPLLKRCGFAVAVGNARQELKDRA
                     HYVTEAYGGDGAVREAIEVILKSSGKWDKVLTKFGFI"
BASE COUNT          371 a          422 c          492 g          482 t
ORIGIN      
        1 cttttatcga tgatgttgta accgcgcagg tcgttggcat cccagttgat cattgaaata
       61 tcgaatttat aaacactgcc gtcaaagagg cttttgtcta tcagtcttct gtaggtccag
      121 aaaggcagga agtttttcac attttcgggt cttgccggat caaaaccttc gtagtgttcg
      181 aacattttga agccgaagag tgaaaatttc cccgcgtctt tgaaagtttc gtagtcgtct
      241 ggtttttcaa tggtgtgatc ttcgcctgca tgcaaagtca gcacgaaagg ataggtgaag
      301 tcttgcacgt tctcggagtc gccctgctct ggggcatggc tctcgtttgt cagtctcttg
      361 ctgtcggagc cggtgtggta gggcactttc accaggggaa agaggtcgcc cagttcggtg
      421 gcatcgagca ctgtcttggg gcagatctct gtgaactggc cgttttctac attaaccgcg
      481 agcagcgctt cgatacgatt atcttcccgg cagtgcgcat ccactatttg gctgcgtgtg
      541 agaatcgtca agcgacctgc ggcaatatag ggggctagcg tctggtctat tacggcaagg
      601 gcccttgccg gttcgaagga aagtctcgtc acccatgatc taccaggatg caaaagggga
      661 tcctgggctg cttccggcgt aagaccacca gtctgcctgt agctttctct aagcttgtcg
      721 cgcaattgca aataactgag gcaagcgccc gtgttttcca cccacttgtt ctcatccagg
      781 gctgagactc cctggctcgt catttgtccg cctagccagc tcgtttcttc cgtcatgata
      841 acggtcccac cgctgtttgc cgtgagggcg gtgagggctg ctgcgacacc gcccatgcca
      901 ccgcctatga tggcgatgtc agccgccagg ctgggaactt cttgtgcttg cggtgaagta
      961 aatgatttca gggctccgtc agccgctgca gcctgcactg atacggcttc tacatctagt
     1021 atttggtaag tctgtgcgcc gctaaaatac ttcacttctg cctgtgcctt tctgctgttg
     1081 ccgagtgttc ccaggaaaca ggtgttatta tatgcaattt gagtgaattg gcgatggagg
     1141 tcagggcatg gcggcaggta ggaaggttgt cgagagtaga tcggttgact tgagcctgct
     1201 ttcagacgag gtcaagaaac gggcgagccg cgtcaaactt gtcttgatgg atgttgacgg
     1261 tgtgctcaca gacggcaccc tttattattt cccaggaccg gacggcaaac ccaccgagtt
     1321 taaaggcttc aactctcaag atggagccgg gctgcatttt ctcaacttgt gcggtatcaa
     1381 aaccggtgtt atcagcggtc gtgattctca ggcggttaat gaaagagccc gcattttgaa
     1441 gattacctat atctaccagg gttaccttga gaaagagagc atttacgaag aaatcctctc
     1501 caaggaaggc gtcgatgatg atgcggtttg ctttatcggt gacgatttcc ctgactaccc
     1561 gcttttgaag cgttgtggtt tcgccgtggc tgtgggtaat gccaggcagg aattgaaaga
     1621 ccgggcccac tacgtcactg aagcctacgg cggcgacggt gctgtgcgtg aggctattga
     1681 agtgatactt aaatcaagcg gcaagtggga caaggttttg accaagttcg gctttattta
     1741 gccgtcgttg tttttgattg gggattt
//
LOCUS       DAOQNZ010000968         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_48966_length_1767_cov_2.831192, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000968 DAOQNZ010000000
VERSION     DAOQNZ010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48966_length_1767_cov_2.831192"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..646)
                     /locus_tag="PKW73_15390"
     CDS             complement(17..646)
                     /locus_tag="PKW73_15390"
                     /inference="COORDINATES: protein motif:HMM:NF016705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNA74731.1"
                     /translation="MRTKAWFFIGALGLAGVAAAAAASESLQHYEEPAYKTELEDGAF
                     ELRSYPAVIAASVQVAGNGDGAANNAFKILAGYIFGKNKAQKKIAMTAPVVQETSEKI
                     AMTVPVTVEKLNMSMTMSFYMPSKYTLQSLPEPLDKRIKLSQVPPRKFAVLRFSGFAT
                     EGSCVLKEVELKRWMAARKLEQTGESMRAFYNPPWTLPFLRRNEIWIPL"
     gene            complement(764..>1767)
                     /locus_tag="PKW73_15395"
     CDS             complement(764..>1767)
                     /locus_tag="PKW73_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNA74732.1"
                     /translation="DKGPALSVLYAANYAHKNNIAINVKFVWELEEEIGSPSFEEFLK
                     DNKEKLATDSIVISDTIWVSRERPAIPYGLRGLQGVLLKLKTGTKDVHSGLTGGLARN
                     PIGELCQLISQCYDAKTGQVHIPGFYDGANEPTDEEAGQFVKSGFTVDGFKKAHELIS
                     LRTSDAKDGSLRIWAKPTFEVHGITGGYQGPGVKTIVPHQAEAKISMRLVPNQDPEKV
                     YQALEKFVKEKAPDVIIEKEGSLKPFLGEFKGPFADAAVGAMKEAFGAEPAFTREGGS
                     IGAVVSMKEVLGKPIVFLGLSLPEHGYHAINENFDWQQASGGMKMFVSYFERLAKIK"
BASE COUNT          418 a          465 c          425 g          459 t
ORIGIN      
        1 gatggcacga gccgcctcat agcggaatcc agatttcatt gcgccgcaag aatggcaaag
       61 tccacggcgg attgtaaaaa gcgcgcatag attcgcctgt ctgctctaat ttcctagccg
      121 ccatccacct cttcaattca acttctttca gaacgcagct cccttcagtg gcaaatccgg
      181 aaaaccttaa taccgcgaat ttcctgggtg gaacctgaga gagcttgatg cgcttgtcta
      241 aaggctcggg caaggattga agcgtatatt tagacggcat atagaagctc atagtcatgc
      301 tcatattgag cttctcgacc gtgaccggaa cggtcatggc aatcttttcg gaagtctcct
      361 gtacaaccgg tgcggtcatc gctatttttt tctgcgcctt attctttccg aagatataac
      421 cggccaggat tttaaaagca ttgttggccg cgccatcgcc gttgcctgca acctgaacgg
      481 aggctgcaat gacagctgga taagaacgca attcgaatgc gccgtcttcc agttcagtct
      541 tatacgccgg ctcttcgtaa tgctgaagac tttcactggc agccgcagcg gcagcgacac
      601 cggccagtcc cagtgcgcct atgaagaacc aagctttagt acgcacggca aaaacctcaa
      661 gaagtaatag agccagtatg caggacttgg gttgcgagta aatgcctaca cagcctctct
      721 tatcagagta tgacagctta gtgaacagac agtgaacggc tgattatttg atttttgcca
      781 atcgctcaaa ataggagacg aacatcttca taccgcctga agcttgctgc cagtcgaaat
      841 tttcattgat ggcgtgataa ccatgctcgg gaagcgagag cccgagaaat acaattggtt
      901 tgccgagcac ttccttcatg ctgacgacag ctccgattga accgccttcg cgagtaaagg
      961 caggctctgc gccaaaggcc tctttcattg ctccaacggc agcgtcggcg aaaggtcctt
     1021 tgaattcacc caggaaaggt ttcaaactac cctctttctc aatgatgacg tcaggcgctt
     1081 tctccttgac gaacttctcc agagcttgat agaccttttc tggatcctga ttgggaacaa
     1141 ggcgcatgct gattttcgcc tcggcctggt gaggcactat tgttttgact ccaggcccct
     1201 ggtagccgcc cgtgattccg tgcacttcga aagtcggttt tgcccagatt ctgagcgaac
     1261 cgtctttggc atcgctcgtg cgcaaggaaa tcaattcgtg agctttcttg aatccatcga
     1321 cagtgaatcc cgacttcacg aattgaccgg cttcttcgtc agtcggctca tttgcgccat
     1381 catagaagcc tggaatatgc acttgtcccg tctttgcatc gtagcactgg ctgatcaact
     1441 ggcatagctc tccaatcggg tttctggcca gtcctccggt caagcctgag tgcacatctt
     1501 tggtgccggt tttcagcttt aatagaacgc cctgcaagcc acgcaaacca taaggaatgg
     1561 ccggacgctc acgagaaacc cagatggtgt cggaaatgac aatagagtct gtggcaagct
     1621 tctctttgtt gtctttcaaa aactcttcga aagaggggct gccaatttct tcttccagtt
     1681 cccagacgaa tttcacgttg atggcaatat tgtttttgtg cgcgtaattc gctgcgtaaa
     1741 gcactgagag tgccggtcct ttgtcgt
//
LOCUS       DAOQNZ010000969         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49019_length_1766_cov_3.111631, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000969 DAOQNZ010000000
VERSION     DAOQNZ010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49019_length_1766_cov_3.111631"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKW73_15400"
     CDS             complement(<1..520)
                     /locus_tag="PKW73_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74733.1"
                     /translation="MVAEIEPAKAVPDARAFLLKLFASRFGSRLSTTGTDVWRSLSQF
                     YALSDEELLASIENGAKTYRALCIDDREAFLLFSFSPQGRDEDVMVWQAFTEAMARLG
                     VNNLRLFQLSEADDYQVYLHFEKLSKIAPLQELFSAYLAASGLERVKVHGAGDYFVLP
                     LQSGFRWMNEAVQ"
     gene            964..1539
                     /locus_tag="PKW73_15405"
     CDS             964..1539
                     /locus_tag="PKW73_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNA74734.1"
                     /translation="MSKIWFSADSHFGHRNIINYCHRPYRNIEEMDRDLIENWNMVVE
                     ESDEVYYLGDFALDFKRVRQVAPLLKGKIHLVAGNHDLCHSSNQGSGSYHRHYMAAGF
                     VDICESTSLEIAGEEVLLCHLPYFDPLDPDQRLPEYKPDDRGNWLLHGHVHARWKTSG
                     RQINVGVDVWDFFPVSLEDIAALIKSAQAEI"
     gene            complement(1555..>1766)
                     /locus_tag="PKW73_15410"
     CDS             complement(1555..>1766)
                     /locus_tag="PKW73_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA74735.1"
                     /translation="GMADEAREKLFTNFRQGQSGPFGSGTGLGLYLCYQIVQAHGGEL
                     SCESKLGYGSTFTLDLPSVSSAGHR"
BASE COUNT          489 a          450 c          444 g          383 t
ORIGIN      
        1 gttgcacggc ctcgttcatc cagcgaaagc cgctctgcaa cggtaaaaca aaatagtcac
       61 cggcgccgtg cactttcact cgctccaagc cggaggctgc caggtaagcc gagaaaagtt
      121 cttgcaaagg cgcaattttg cttaacttct caaaatgcaa ataaacttga tagtcgtctg
      181 cttcactgag ctgaaaaagt ctcagattgt tgacgccaag tcttgccatt gcttcagtga
      241 atgcctgcca taccatgacg tcttcgtcgc gcccctgggg cgaaaacgaa aagagcaaga
      301 aggcttcccg atcatcaatg caaagagcac ggtaggtttt ggcaccgttt tcgatagatg
      361 ccagcagttc ttcatcgcta agagcataga actgactgag actgcgccaa acatcggtac
      421 cggtagtaga aagcctggaa ccaaagcgag aggcaaacaa tttcaacaag aaagcgcggg
      481 catcgggcac cgcttttgcc ggctcaatct ctgctaccaa tggtggtacc agtccgccat
      541 tggctgaatt gacgttagcg ttagtagaag tagtactaat tttaccgctc gctttctagc
      601 acgattggat atacaagaag gatggtattg ggagcccgga aggaacccgg agaccagcct
      661 gttaaccgtc taatgcaccc ttacaaaaca ccagaactac aataagggct tgagactgag
      721 caagtgattc ccgaacgcgt cacattttat catgtcagca ggatgtaacc acaggcggtg
      781 attatttaca agtcatacaa agactatatc ccgggaattt cccaggttaa ttagagcgcc
      841 cgcatatcca cttgcacgca tatttcaaat ctggtgtaaa actgaagtag taacttcaat
      901 gttacaagtg gttacctaaa ataaagccgc tcagtctcaa agtcggaaag accgacaaaa
      961 tctatgagca aaatctggtt tagcgccgat tctcatttcg gccacagaaa tatcatcaat
     1021 tactgccacc gcccctaccg gaacatcgaa gaaatggacc gggacctgat tgagaactgg
     1081 aatatggtgg tggaagagag cgacgaggtc tactacctgg gagacttcgc cctcgatttc
     1141 aagcgggtgc ggcaggtggc gccgctcttg aaggggaaaa tccatctggt tgccggcaac
     1201 cacgatctct gccattccag taaccagggt tccggttctt atcatcgtca ttatatggca
     1261 gcaggctttg tagacatttg cgagagcacc agcctggaaa tagccgggga agaggttcta
     1321 ctctgccatc taccctactt cgacccgctt gatcccgatc agcggctgcc cgagtacaaa
     1381 cccgacgacc ggggcaactg gctcttgcac gggcacgttc acgccagatg gaaaaccagc
     1441 ggcaggcaaa tcaatgtggg ggtggatgtc tgggattttt tcccggtgag cctggaagac
     1501 attgccgccc tgatcaagag tgcacaagcg gaaatatgaa atgggaggcg aaaactagcg
     1561 gtgaccggca gaagaaaccg acggcaggtc aagtgtaaaa gtagatccgt aacccaactt
     1621 gctttcacag gaaagctcgc ctccgtgggc ctggacaatc tgatagcaaa gatatagacc
     1681 caggccggtt ccggagccaa aggggccgct ctgcccctga cgaaaattgg taaaaagctt
     1741 ttcccttgct tcatcggcca tacccg
//
LOCUS       DAOQNZ010000970         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49024_length_1766_cov_2.970193, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000970 DAOQNZ010000000
VERSION     DAOQNZ010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49024_length_1766_cov_2.970193"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKW73_15415"
     CDS             <1..724
                     /locus_tag="PKW73_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNA74736.1"
                     /translation="VAHPPHLAIVEAKVVADLASQHGAEKFVLVSSDKAVNPTSVMGA
                     TKRMAELVIQDLALRAGKTKYVAVRFGNVLASRGSVIPLWRQQIAAGGPLTVTDPEVT
                     RYFMLIPEAVQLILQAGAYGSGGEIFVLDMGKPVKILDLANDLIKFSGLRPGHDIKIQ
                     FTGLRPGEKLYEELLTEDEGLSRTVNEKIFVGRPKPLEPEALAEALGRFESYVASLDE
                     AGIRAELDRLAAGSLTAAQMEV"
     gene            728..1603
                     /gene="larE"
                     /locus_tag="PKW73_15420"
     CDS             728..1603
                     /gene="larE"
                     /locus_tag="PKW73_15420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNA74737.1"
                     /translation="MTGSCLDSPEQPAELLAKDQRLRAEIRQLGSVLVAYSGGVDSSY
                     LALVAFQELSEAALAVIAVSPSLAQAELVDARRQAEQMGFPLLEIATNEVENELYRAN
                     GGNRCYFCKTTLFDSMSLLKQERGFAAIAYGANMDDLSDVRPGHAAARAAGVLAPFVN
                     CHLYKEEIRTLAALHGLPSWDRPQAACLSSRFPDFTPIDASRLALVERAEALVKAAGF
                     KQVRVRFLSRPEGLTASVEVGQDELALLTPSLTEPLSLAIRALGFEFVQFDKAGYRQG
                     NANAVLFEPAVRSGI"
BASE COUNT          340 a          484 c          509 g          433 t
ORIGIN      
        1 agtagcccat cctcctcatc tggcgatcgt cgaggccaaa gtggtggccg acctggccag
       61 ccaacacggc gccgagaaat ttgtcctggt ttcatcggat aaagccgtta atcctacttc
      121 cgtcatgggc gccaccaagc gcatggctga gcttgtcatt caagaccttg ctctccgtgc
      181 cggtaagacg aaatatgtgg cggtcaggtt tggtaatgtg ctggccagcc gcggttcagt
      241 aatcccgctc tggcggcagc aaatcgccgc cggtggaccc ctgaccgtca ccgatccgga
      301 agtgacccgc tacttcatgt tgataccgga ggcggtgcag ctcattttgc aggccggcgc
      361 ctacggctcc ggtggcgaga tcttcgtgct ggatatgggc aagccggtca agatactcga
      421 tctggctaat gatttgatca agttttccgg tttacgccct ggtcacgaca tcaaaattca
      481 atttaccggt ctaagacctg gtgagaagct ttacgaggag ctgcttaccg aggatgaagg
      541 gctttcccgc acggttaacg aaaagatttt cgtaggacgc cccaagccat tggagcccga
      601 agcgctggct gaggcccttg gtcgttttga gtcatatgtg gccagtcttg acgaggccgg
      661 tattcgcgcc gagctggatc ggttggctgc gggttctctc actgctgcac aaatggaagt
      721 ctagattgtg actggctctt gcctagattc gccggagcaa ccagcggagc tactggctaa
      781 ggaccagcgc ttgcgagccg agatcaggca gcttggctca gtactggtgg cttattccgg
      841 tggagttgat agctcctatc tggcgctcgt tgcctttcag gaactttccg aggcggccct
      901 tgccgttatc gccgtctctc cctccctggc gcaggctgaa cttgtggacg ccaggcggca
      961 ggcggagcaa atgggctttc cccttctgga aattgccaca aatgaagtgg agaacgagct
     1021 gtatagagca aacggcggca atcgttgcta cttttgcaag accaccttgt ttgacagtat
     1081 gtctctcctc aagcaagagc gtggtttcgc tgccatcgcc tatggtgcca atatggacga
     1141 tctgtccgat gttcgcccgg gtcacgctgc cgcccgtgct gccggtgttc tcgctccttt
     1201 tgtcaattgc catttgtaca aagaggaaat tcgcaccctg gccgcactgc atggtttgcc
     1261 ttcctgggat cgaccgcaag cggcctgcct ttcctcacgc ttccctgatt ttacgcccat
     1321 agatgcctct cgcctggctc tcgtggaaag ggctgaagct ctcgtcaaag cagccggatt
     1381 taagcaagtg cgtgtgcgct tcttgagccg accggaaggt ctgactgctt cggtggaagt
     1441 aggtcaggat gaactggcgc tacttacccc ctccctgacc gagccattga gccttgctat
     1501 ccgcgctctc ggttttgagt ttgttcagtt cgacaaagcc ggctatcggc agggaaacgc
     1561 caatgccgtc ttgtttgagc ctgccgttcg gtctggtatc tgaaaatgga tagattgcat
     1621 ttagagagac ttttgcagtc ggttgccgat ggaaatctaa ccgtggaggc tgctcttgct
     1681 agtttgcaaa aaaggctttt ggcgccggag agggcgcggg aactggattt tgccagaccc
     1741 gaccttcagc gcgcctggcg tcagag
//
LOCUS       DAOQNZ010000971         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49040_length_1766_cov_2.384570, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000971 DAOQNZ010000000
VERSION     DAOQNZ010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49040_length_1766_cov_2.384570"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1090)
                     /locus_tag="PKW73_15425"
     CDS             complement(32..1090)
                     /locus_tag="PKW73_15425"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74738.1"
                     /translation="MQKKTRRRCKSYTPIFTLCLSLLLTGASAKAEDRAGQISALKNK
                     SMLMANTKKFKEAIALSEQALKLDKHDATIFYLRARIKEEQKDPVAAIKELNRAIELN
                     PKYAEAYLLRAKGYYFLAEMDEGRDNSEKAKSDLATAIKLNPRLAEAYDYKGVGLASE
                     NKLKEALDCFNQAVTLNNNNGTFLDHRAMALNALGKRLEATKDLRRSAELNKNDPHGW
                     VILSQQFTDIGMYKDALISLNMAIKLKPHQLDNIYKRALLYQKLHMYKEALADLNKVL
                     ESSPSDDDAFRMRGDCYVGLKDFPLALKDYDQAIKLSGAQASHYQARAKLHRLMGKNA
                     AASKDESTAAKLKAEKPK"
     gene            complement(1110..>1766)
                     /locus_tag="PKW73_15430"
     CDS             complement(1110..>1766)
                     /locus_tag="PKW73_15430"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74739.1"
                     /translation="EDINKALVLDPNLAEAYHSKGVWLSLEQHNKEAIHYFSKAIELE
                     KTNPTYYTHRANSYNSIGDSISALKDLNAAVALRPRDKNVHLDRAKLLTQMKKYDDAL
                     KDYLDALKIDPRSTTARSELTVLYQKMGRYNEAVDEYTRIIKASPLDDEAYQSRGKCY
                     QAMKKFDLALSDFNEAIELSPDLPSHYEARAKLYKVMNKVELYNKDSLMAQNLREKKP
                     "
BASE COUNT          333 a          407 c          462 g          564 t
ORIGIN      
        1 agccgaaaag gcttgatttc gacctgtcag actatttcgg tttttcagct ttcaattttg
       61 cggccgtact ttcgtctttg gaagcggccg cattttttcc catcagtctg tgaagcttcg
      121 ctctcgcctg gtagtgggaa gcttgcgcgc cggatagttt gatggcctgg tcgtagtcct
      181 tgagggcaag gggaaagtct ttcagtccta catagcaatc gccgcgcatt ctaaaagcat
      241 cgtcatcgga tggactgctc tccagaactt tgttcaggtc ggccagggcc tctttgtaca
      301 tatgcagttt ctggtagagc agcgcccgtt tgtagatgtt atcgagttgg tggggcttta
      361 gtttgatggc catgttcaag ctaatgaggg cgtctttgta catgccgata tcggtgaatt
      421 gttgcgataa aattacccaa ccgtgcgggt cgttcttgtt gagttcggcg gatcgcctca
      481 ggtctttcgt cgcttccagg cgtttgccca gagcattgag ggccatagcc cggtgatcga
      541 gaaaggtacc gttattgttg ttcagagtca ctgcttgatt aaagcagtcg agggcttctt
      601 tcaatttgtt ttcgctggcc agcccgacgc ccttatagtc ataggcttcc gccagccgcg
      661 gatttaattt gatggccgtg gccagatcgc ttttggcctt ttcggaatta tctcttcctt
      721 catccatttc ggccaggaaa taataccctt ttgctcgcag caggtaggct tcggcatatt
      781 tgggattgag ttcgattgcc ctgttcaact cttttatggc agctactgga tctttttgct
      841 cttctttgat tctggctctt aagtagaaga tcgtggcatc atgcttgtcg agcttgagag
      901 cctgttcgct cagggctatg gcttccttga acttctttgt attggccatg agcatgcttt
      961 tgtttttcag tgcacttatt tggccggctc tatcctcagc ctttgccgac gctcctgtta
     1021 aaagtaagga caggcagagg gtaaatatgg gcgtataact tttgcagcgc cgcctggttt
     1081 tcttttgcat cagcaccggc acctctgtct taaggtttct tctctcgcag attttgggcc
     1141 atgagggagt ctttgttata gagttcaacc ttgttcatta ctttatacaa cttggctcgt
     1201 gcttcatagt gggaaggcag atccggcgac aattctatag cttcgttgaa gtcgctcagg
     1261 gccaggtcga atttcttcat ggcttgataa catttgcccc ggctctggta agcctcatcg
     1321 tctaaaggcg aggctttgat gattcgggta tattcgtcaa cggcttcgtt ataacgtccc
     1381 attttctggt agagcacagt cagttcgctg cgagcggtgg tgcttctggg atcgattttg
     1441 agggcgtcaa ggtagtcttt caaggcatcg tcgtacttct tcatctgggt gagcaacttg
     1501 gctctgtcca ggtggacgtt tttgtctctc ggcctgagtg caaccgccgc attcaaatct
     1561 ttcagagcgc taatcgaatc tccgatgctg ttataggaat ttgctcggtg cgtgtaataa
     1621 gtgggattcg tcttctcaag ctcaatggcc ttgctgaagt agtgaatcgc ttccttgttg
     1681 tgttgctcca gggagagcca gacacccttg gagtgatagg cttcggctaa attgggatcg
     1741 agaaccaggg ctttattgat atcttc
//
LOCUS       DAOQNZ010000972         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49054_length_1765_cov_5.144444, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000972 DAOQNZ010000000
VERSION     DAOQNZ010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49054_length_1765_cov_5.144444"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PKW73_15435"
     CDS             complement(<1..357)
                     /locus_tag="PKW73_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA74740.1"
                     /translation="MPHSYSKERETATMSSLDIEKIRRDFPILRREIDGKRLVYLDNA
                     ATSQKPLSMINCLNEYYQRYNANVHRGIHTLSEEATALYEGTRETVRRFIDARESCEI
                     IFTRGTTEGINLVAQSW"
     gene            complement(414..734)
                     /locus_tag="PKW73_15440"
     CDS             complement(414..734)
                     /locus_tag="PKW73_15440"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNA74741.1"
                     /translation="MTNSLGSVKIARLDEVEPGKVKVFNLGQEAIALCNVDGKIYAVK
                     DVCTHDDGPLGEGELNGCQIECPRHGARFDVTTGKAMSLPAVLPVPTYKVDVQGSDIF
                     VSLE"
     gene            complement(749..>1765)
                     /gene="sufD"
                     /locus_tag="PKW73_15445"
     CDS             complement(749..>1765)
                     /gene="sufD"
                     /locus_tag="PKW73_15445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNA74742.1"
                     /translation="TAKVVVSPFAQAVESHKAVLEKLFAEKRGIQDKFTLMNEALAGG
                     GLFVHVPKNTRVEAPVVMMLNLPEKATTAAKDPSGLAVFPRVIINVEENASINFVSIV
                     ASEKTAEATETVLAASVVEFHIAAGAKVSVAEVTNLGPEVFFVNRVRAYVARDAAIDF
                     STAGLGGKQTKSDIETILMDRGAHAAVKGVVFGGGAERYAYNTIAEHAAPDTTSNINF
                     RVALKDKSASIYQGIVKVDKVAQRTDAYQSNKNLLLGKEAKADSIPKLEILADDVKCS
                     HGATVSPVDKEQLFYLTCRGLTAPEAEELIVTGFFQQVLDTIPIQGVVNFLAALTSDK
                     LHKS"
BASE COUNT          337 a          432 c          518 g          478 t
ORIGIN      
        1 ccaggactgg gcgacgaggt tgatgccctc cgtggtgccg cgggtgaaga tgatttcgca
       61 agactcgcgg gcgtcgatga aacggcgtac cgtttcgcgg gtgccttcgt agagggctgt
      121 ggcttcttcc gaaagtgtat ggataccacg atgcacgttg gcgttgtagc gctggtagta
      181 ttcattcaag caattgatca tggatagcgg cttctgggat gtagcggcgt tatcgagata
      241 aacgaggcgc ttgccgtcta tttcccgtcg caaaatggga aagtctctgc ggattttctc
      301 tatgtcgaga ctggacatgg tggctgtctc tctttctttg ctgtaggagt gaggcaagtc
      361 tggctccgtt tctgatgttt ttagaggatg attgagggtt tgcatagcgc tttttactct
      421 agcgaaacaa agatgtcgga gccctggacg tctactttgt aggttggtac cggtagtacc
      481 gccggcagtg acatggcttt gcctgtggtg acgtcgaaac gagcgccgtg cctggggcat
      541 tctatctggc aaccgtttag ctcgccttcg cccaggggac cgtcgtcgtg ggtacagaca
      601 tctttgacgg cgtatatttt accgtctaca ttacaaaggg ctattgcttc ttgccccagg
      661 ttgaaaacct tgaccttgcc gggttcgact tcgtctagcc gggctatttt gacgctaccg
      721 aggctgttgg tcatagttca agcccctctt agcttttgtg cagtttgtct gaggtgagcg
      781 cagccaggaa attcactaca ccctgtatgg gaattgtatc cagtacttgt tggaagaagc
      841 ctgtaacaat cagttcttcc gcttccggcg cggtcaaacc gcggcaggtc aggtagaaga
      901 gctgttcttt gtctacggga gaaactgtgg ctccgtgtga gcatttgacg tcgtcggcaa
      961 gaatctccag tttcggtatg gaatcggctt tcgcttcctt cccaagcagt aagttcttgt
     1021 tcgactggta ggcgtcggtg cgctgggcta ctttgtcgac tttgacgatg ccctgataga
     1081 tggaggcgga tttgtctttg agggcaaccc ggaaattgat attggatgtg gtatcaggtg
     1141 cggcatgctc ggcaattgta ttgtaagcgt agcgctcggc gcctccgccg aatacaacac
     1201 ctttgacggc ggcgtgagcg cctctatcca taaggatcgt ctcgatatcg gatttggttt
     1261 gtttaccacc cagaccggcg gtgctgaagt ctatggcggc gtctctggcc acataggctc
     1321 tcacccggtt gacaaagaat acttctggtc ccagattggt cacttccgcc accgatactt
     1381 tggcgccggc ggcaatatgg aactccacta cggaggcagc cagaaccgtt tcagttgctt
     1441 cggcggtctt ttccgacgcg acgatggata cgaaattgat ggaggcgttt tcttctacgt
     1501 tgatgatgac gcgaggaaag actgccagtc cggacggatc tttagcggcc gttgtggcct
     1561 tctccggtag gttgagcatc attaccaccg gggcttccac tctggtgttc ttcggcacgt
     1621 gtacgaagag accgccgccg gccagggctt cattcatgag ggtgaatttg tcctggatgc
     1681 cgcgcttttc ggcaaagagc ttctccagaa cggctttatg actttccacg gcctgggcaa
     1741 agggactgac caccactttg gcggt
//
LOCUS       DAOQNZ010000973         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49186_length_1763_cov_3.366511, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000973 DAOQNZ010000000
VERSION     DAOQNZ010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49186_length_1763_cov_3.366511"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKW73_15450"
     CDS             <1..656
                     /locus_tag="PKW73_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74743.1"
                     /translation="IATTFLRYWSCLLWRISMAVISTRAQRRLGEVTIAAIVIFLAWV
                     IQMSILCKFRFTDAVASLPLAMTIVWGLTFGSPMEKPTADELRVSSVSAIFIRQLFSG
                     SPSGAIVGAFFAALAASVLPIFPVSYPLVGWIAGYFSLRNFNQAAFLCIPLTVVLTFL
                     GEMIMAGQLALTGRDVLGHLVAISVPEAFLNGLISPVLFIPMRGWYSYSLYYRQLGR"
     gene            669..>1763
                     /locus_tag="PKW73_15455"
     CDS             669..>1763
                     /locus_tag="PKW73_15455"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNA74744.1"
                     /translation="MLTETEPFIESTASRRQDYGLKVAVLCTVIGLAMAALVARLVWL
                     QILQNGYYKAQAVENSTRVTFLRAPRGNIYDRHGTLIATNKQSLSMIAVPAQLENVED
                     LANRLTPILSQPQPKILASLLKAKASGSVLPVVIERDLDMETVAKFYERKIFLKGVDI
                     LQDVSRSYPYGEVTAHVLGYCGEITQSQLKRRPGRKMGDVVGQDGVERLYDDQLRGID
                     GEQRVRVNAMGHSLSAETARPQITRQSTAGKPVVLSLDLELQKAAYEALGERSGAIVA
                     IDAQTGEVLAMVSRPSFDPNVFTKRITPAVWKRINAPDHPLFNRALSGYPPGSIWKAF
                     TLLAALETKAVKPDTKLHVSGGISLGGFFFG"
BASE COUNT          337 a          488 c          476 g          462 t
ORIGIN      
        1 taattgctac gacctttctc aggtactggt cttgcctcct ctggagaatt agcatggccg
       61 tcatttccac cagggcacag cgaagattgg gagaggtgac aatagcagcc attgtcatct
      121 ttctggcctg ggttatacaa atgtcgattc tttgtaagtt tcgctttact gatgccgtgg
      181 ctagtttgcc cctggctatg accattgtct gggggctgac cttcggttcg cccatggaga
      241 aacccactgc tgatgaactg cgggtttcca gcgtctcagc catctttatc cgtcagctat
      301 tctccggttc cccttccggc gccatagtcg gtgccttttt tgcagccctg gccgcttccg
      361 ttttgccgat ttttccggtc tcttatcccc ttgtgggctg gatcgccggc tatttctctc
      421 tgcgcaattt caaccaggcc gccttccttt gtataccgct tacggtggtg ctgacgtttt
      481 tgggcgagat gattatggcc ggacaactgg ctttgaccgg tcgagacgta ctgggacatc
      541 tggtcgctat cagtgtgccg gaagcctttc tgaacgggct gatttcgcct gtgctcttca
      601 ttcccatgcg tggttggtat tcctattcac tttattatcg tcaactcggt cgttagtgag
      661 gatggaaatt gctgactgaa accgagccat ttatcgaaag caccgcttcc cgcaggcagg
      721 attacggcct caaggttgcc gtcttgtgca cggtgattgg tctggccatg gccgcattgg
      781 ttgcccgtct ggtctggttg caaattttgc agaacggata ttacaaggct caggccgtgg
      841 aaaactccac ccgcgtaacc tttctgcggg caccgcgggg caatatctat gatcgtcacg
      901 gtactttgat tgccaccaat aagcagtcgc tctcgatgat tgccgtgccg gctcaactgg
      961 agaatgtcga ggatctggcc aaccgcctta ctcccatatt gagccagccc caacccaaga
     1021 ttctcgcatc cctcctcaag gccaaggcct ccggttcggt attgcctgtg gttatagaaa
     1081 gagacctgga catggaaacg gtggccaagt tttatgagcg caaaatcttc ttgaagggcg
     1141 ttgatatctt gcaggacgtc agccgcagct atccctacgg tgaagtaacc gcgcacgtgc
     1201 ttggctattg cggtgagatt acccagagtc agttgaagcg tcgccccggg cgcaagatgg
     1261 gggatgtggt cggtcaggac ggggtggaaa gactctacga tgatcaactg cgcggcatcg
     1321 acggggaaca gcgggtgcgg gtcaatgcca tgggccattc cctttcggct gaaacggcca
     1381 gaccgcaaat caccagacag tccacggccg gcaaaccggt cgtgctctcc ctcgatctgg
     1441 aattgcagaa agctgcttat gaggctctgg gtgagcgctc cggcgctatc gtggccatcg
     1501 atgcccagac cggcgaggta ctggctatgg tgtcacggcc gagtttcgat cctaatgtat
     1561 tcaccaaacg tattacgccg gccgtttgga agcgcatcaa tgcgcccgac cacccgctct
     1621 ttaaccgggc tctttccggc tacccgcctg gttctatctg gaaagccttt acccttctgg
     1681 cggccctcga aacaaaagcc gttaaaccgg ataccaaatt gcatgtctcc ggtggtatct
     1741 cccttggtgg tttcttcttt ggt
//
LOCUS       DAOQNZ010000974         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49261_length_1762_cov_2.696544, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000974 DAOQNZ010000000
VERSION     DAOQNZ010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49261_length_1762_cov_2.696544"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PKW73_15460"
     CDS             complement(<1..642)
                     /locus_tag="PKW73_15460"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA74745.1"
                     /translation="MTQGKRSILITGGAGFIGSHLSERFLSLGDCVTVIDNFNDFYNP
                     LLKRRNVAAFAGNANYNLVEGDIRNSEDLDRAFSHGPFDVVIHLAAMAGVRPSLEQPA
                     YYFDVNVNGTQKLLDHILPTVERTRLVFASSSSVYGERAGEPFKETDRVCEPISPYAA
                     SKASNELQLYACHYATGLAVTCLRFFTVFGPRQRPDLAIHKFCHLIDAGKPVEL"
     gene            complement(639..1424)
                     /locus_tag="PKW73_15465"
     CDS             complement(639..1424)
                     /locus_tag="PKW73_15465"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNA74746.1"
                     /translation="MNKADRKPLIGINLDIKGGPPEEAAIQTTYTDAILKSGGVPVLL
                     PPMEEADLYIALSRLDGLMLIGGADYCPSLYNEEPHDKLDLAHDRRQQFDLALARQAL
                     ANQHLPILGICLGAQLINILQGGSLVQDIKTFIPESTIEHVSVNGWKDGFTQHPVKLL
                     PDTQIARIYGNNNFAVPTSHHQSVKQLGRGLMVSAEAEDGVVEAIEMPERTFVIGVQW
                     HPERDYPTNKPLFDELVAVSLNNLSTNSSPSNLLSADNIRNRS"
BASE COUNT          431 a          449 c          482 g          400 t
ORIGIN      
        1 gagttccacc ggcttaccgg cgtcgatcaa atggcaaaac ttgtgaatag ccagatctgg
       61 tctctggcgg ggcccgaaga ccgtaaaaaa tcgcaggcag gtaacggcca gaccggtagc
      121 ataatggcag gcataaagct gcaattcatt ggaagctttg ctggccgcat agggtgaaat
      181 cggctcacaa acccggtccg tctccttaaa aggctctccg gcccgttccc cgtataccga
      241 actggaagag gcaaaaacca gtcgagtgcg ctctaccgtc ggcagaatat gatcaagcag
      301 cttctgggtg ccgttgacgt tcacatcaaa gtaataggca ggctgctcca gagagggacg
      361 caccccggcc atggccgcca gatggatgac cacgtcgaaa ggaccgtgac tgaaggctct
      421 atcgaggtct tcgctgtttc taatatcgcc ttccaccaga ttgtaattgg cattcccggc
      481 aaaagcggcg acgttgcggc gcttcagaag cggattgtag aaatcattga agttgtctat
      541 gacggtgacg cagtctccca gagaaagaaa ccgctcactc aaatgactac cgataaagcc
      601 ggcgccgccg gtaattaaga tgctgcgttt tccctgcgtc atgagcggtt ccttatattg
      661 tctgccgata gaagattgga aggcgacgaa ttggtcgaga ggttgttcaa agacacggct
      721 accagttcat cgaaaagagg cttgttggta gggtaatccc gctcaggatg ccactgcacg
      781 ccaatgacaa aagtacgttc cggcatttca atcgcttcca caaccccgtc ttccgcttca
      841 gcggaaacca tcaagccgcg ccccagttgc ttgacgctct ggtgatggga ggtgggcact
      901 gcaaaattgt tattgccgta aatacgggct atctgcgtgt ccggtaagag tttgaccgga
      961 tgctgagtga agccgtcctt ccaaccattg acgctgacat gctcaatagt ggattcagga
     1021 atgaaggttt tgatgtcctg aaccagggag ccgccctgca agatattgat caactgcgca
     1081 cccagacaga ttcccagaat cggcaagtgc tgattggcaa gagcctgacg agcgagagcc
     1141 agatcaaact gctgacggcg gtcgtgggcc aggtcaagct tgtcgtgggg ctcttcgttg
     1201 taaagggaag ggcagtagtc agccccgcca atcagcatca gtccgtccag tcggctcaaa
     1261 gcgatataaa gatcggcttc ctccatgggc ggcaacagga ccggcacgcc gccgcttttc
     1321 aagattgcgt cggtataagt agtctgaata gcggcctctt ccggaggtcc ccctttgata
     1381 tctagattta ttccaattaa gggctttcta tccgctttat tcaccgttcc gttcctatgc
     1441 ttcctgaaga gattcctgac ttggttcggg tttggagctg atttagttca gtctcatgaa
     1501 agcaattgat ccggcctcag ggctgttccg ccattggaaa gcgcttccgc gccgggcttg
     1561 cctttgtgag aggcaccgct cccgaaaggg ctattttaaa ccaattccag cccgctctat
     1621 tgctccaaag gaccgcggcg gagaaatcaa ttttaaaatt atgtgggaac tttcatgcaa
     1681 gtaggcaggg gccgggtact aacataagac cgttgagcag ggttattgaa tgagaggggg
     1741 caaaagtgaa gccggaagaa ct
//
LOCUS       DAOQNZ010000975         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49352_length_1760_cov_3.870381, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000975 DAOQNZ010000000
VERSION     DAOQNZ010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49352_length_1760_cov_3.870381"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PKW73_15470"
     CDS             <1..1031
                     /locus_tag="PKW73_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNA74747.1"
                     /translation="SAPKPQSRILGLLLLVCQPCERGSQNLTELKAAANIFAALVAPV
                     LSSSQGAPRFDEPPAISEMSRRLSAELWLRQAICKLHLSLDRDCILQTLVDSLGRGLR
                     PSRCLVVRTSPGAPELVCHEYEDPDLSPLGLGRTNQFPLFAVQLLRHRCTAYGDVSEL
                     LAAGAITEEQYDVLSFGGIVSLAGAPLYCQDQLFGVVVLVDAQKRLWDKTELDGLDTL
                     ASQAAIALTHSATYEESQHQLFHLSLISNVTEQLTSALDMVSHLPRVGRPEERSVPET
                     VETASLSARELEVLRLISSGYANKEIAQRLFLTESTVELHASRIRKKLKLKSRTALVK
                     YACDKGIV"
     gene            complement(1028..>1760)
                     /locus_tag="PKW73_15475"
     CDS             complement(1028..>1760)
                     /locus_tag="PKW73_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74748.1"
                     /translation="ESLQDDKELKLWQPNAAQAAMRVASDDWPFLYLQFDTTGLILYS
                     TFLLFTIIIPVPFLFKVEKSSVLAPQCFAMFVLGEAFMLMETKCCTELSLLYGNTWLV
                     SSVVIFSFLTLGFLANLLVLKLENEKLTRILPLIYLSLSVILLADFFLSMRNIALPAA
                     GAKPLTTLLTCLPVFFGGLLYSSHFKTVKDANMALAANLLGVTFGGLLENLCVVGGIK
                     SLSLLTLILYLLSALPLIKGKADKN"
BASE COUNT          399 a          468 c          506 g          387 t
ORIGIN      
        1 cctctgcacc taagccccag agccgcatac tgggtttact tctgcttgtc tgccaaccgt
       61 gcgagagggg gtcccagaat cttactgagc taaaggcggc agccaatatt tttgctgctc
      121 tggtggctcc cgttctttct tcttcccagg gagcgccgag gttcgacgaa ccgccggcca
      181 tatccgagat gtcccggcgg ttgagcgccg aattgtggct ccggcaggct atttgcaaat
      241 tgcatctctc gctcgatcgc gattgtattt tgcagaccct ggtggacagc ctggggcgcg
      301 gtctcagacc gagccgttgt ctggtggttc gcaccagccc gggcgcgcct gaattggttt
      361 gccacgagta cgaagatccc gacctgtctc ccctggggct gggtcgcacc aaccagtttc
      421 ccctctttgc cgtgcaattg ctcaggcacc ggtgcactgc ctacggcgat gtttccgaac
      481 ttctggccgc cggtgccatt accgaagagc aatacgatgt gcttagtttc ggcggtatcg
      541 tcagtctggc tggggcaccg ctttactgtc aggaccaact ttttggagta gtggtactgg
      601 tagatgccca gaaacgactc tgggataaaa ctgaattgga tggtctggat acgctagctt
      661 cgcaggcggc cattgccctc acccattccg ccacttatga ggaaagtcag caccagctct
      721 tccatcttag tctgatcagc aatgtcaccg agcaacttac ttcggcactg gatatggtgt
      781 cgcatttgcc ccgcgtcggt agaccggagg agcgaagcgt acctgaaaca gtcgaaacgg
      841 ccagtttgtc cgccagagag cttgaagtgt tgcgcctgat cagctccggc tatgccaaca
      901 aggaaatcgc ccagcgtctt ttcctgactg aatccaccgt ggaactacac gcttctcgca
      961 ttcgcaagaa actgaagctg aagagccgaa ctgcccttgt taaatatgcc tgcgacaaag
     1021 gcatagtcta gtttttatcg gctttccctt tgatcagggg cagtgccgac agcaggtaga
     1081 gaatgagcgt gagtagactc aagctcttga tgccgcctac aacgcacaga ttctccagca
     1141 gaccaccaaa agtgacaccc agtaagttgg cggccagcgc catgttggca tccttgacgg
     1201 tcttaaagtg agaagaatag agcagtccgc cgaaaaatac gggtagacag gtcagaagcg
     1261 tcgtgagcgg cttggcaccg gctgccggca gggctatgtt gcgcatggaa aggaagaagt
     1321 cggccagcaa tatgaccgac agcgatagat aaatcagggg caggatgcgg gtcaattttt
     1381 cgttttccag cttcaagacc agcaggtttg ccaggaagcc cagggtcagg aaggagaaga
     1441 tgaccaccga actgaccagc caggtgttgc cgtagagcag tgagagttca gtgcagcact
     1501 tcgtttccat aagcatgaaa gcctctccca gaacgaacat ggcgaagcac tgcggggcaa
     1561 gcaccgagga cttctccact ttgaagagga agggcacggg aatgatgatg gtgaagagca
     1621 ggaaggtgct gtagaggatg agaccggtgg tgtcaaattg cagatagaga aacggccagt
     1681 catcgctggc tacccgcatg gcggcttgag cggcattggg ctgccagagt ttcagctctt
     1741 tatcgtcctg aagggactct
//
LOCUS       DAOQNZ010000976         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49383_length_1760_cov_2.597067, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000976 DAOQNZ010000000
VERSION     DAOQNZ010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49383_length_1760_cov_2.597067"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PKW73_15480"
     CDS             <1..607
                     /locus_tag="PKW73_15480"
                     /inference="COORDINATES: protein motif:HMM:NF013626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74749.1"
                     /translation="TGTMAYPRLGPAAELISLVETFTPEIEQLGQSKVDASKPGRGGI
                     NTKIAAAKVATQSGCAAIIAGGKVEGVIRRIFSGKEVGTLFLPGKGLSGKQRWIAFAT
                     TVKAALVVNSGARTALLGGKASLLPAGVVAVKGNFDRGDVVSIEDEGGREFARGIVNY
                     ASDETRQVSGQSSDALENLVSPLGRNYDALITRDNIAFLEV"
     gene            613..>1760
                     /locus_tag="PKW73_15485"
     CDS             613..>1760
                     /locus_tag="PKW73_15485"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNA74750.1"
                     /translation="MTASNETLTLAKEARRGARSLAASSLTERNQILSRYAQLLEENA
                     DRIFAANAEDMAAESDENLLKRLKFDQSKLQDVLSGLKALIAMADPLSRVDLRTKLDT
                     GLVLERVSCAIGVLAVIFEARPDALPQIAALSIKSGNAALLKGGKEASRTNQVLFDLL
                     KESLGTFADCFHLLSGREAVEALLKARGDVDLIIPRGSGSLVSYIQSNTQIPVLGHAE
                     GVCHIYIDEGADAAKSLDVVLDAKVSYPAACNAVETLLVHDEFDRGHLQSIFERLLAE
                     KVELRVCPELMKLFAGTELSGKLVKAKPSDFGTEFSARILACKQVKSLDEAIDHINKY
                     GSGHTDAILTENDDHWRRFFSQVDSAGVYRNCSTRFADGFRYGFGAEVG"
BASE COUNT          406 a          452 c          493 g          409 t
ORIGIN      
        1 gacgggtacc atggcatatc cgcgccttgg accggcggca gaactgatta gtctggtaga
       61 aacattcaca ccggagattg agcaactggg gcagtctaaa gttgatgcct ccaagcccgg
      121 tcgaggtggt atcaatacca aaatcgctgc cgccaaagta gccacccaga gcggctgcgc
      181 cgccataatt gccggtggca aagtggaagg cgtgattcgc cggatttttt ccggcaaaga
      241 ggtgggtacc ctcttcttgc caggcaaagg gctttccggc aagcagcgct ggatagcctt
      301 tgccaccaca gtgaaagccg ccctggtggt aaattccgga gctagaacag cacttctggg
      361 aggcaaagcc agtttgctgc cggccggtgt ggtagcggtg aaaggcaact tcgaccgggg
      421 tgatgtcgta tccatagaag atgaaggcgg ccgtgaattc gcccggggta tagtcaacta
      481 cgccagtgat gaaacccggc aggtctcagg gcagagttcc gatgccctgg aaaatctggt
      541 tagccccctt ggacgcaatt acgatgccct gattacccgt gacaatattg catttttaga
      601 ggtatagagc agatgaccgc tagcaatgaa accctgaccc tggctaaaga ggctcgccga
      661 ggcgcaaggt ctcttgctgc cagcagcctg actgaacgca atcagattct tagtcgctac
      721 gctcaacttt tagaggaaaa tgccgaccgc atcttcgccg ccaatgcgga agatatggcc
      781 gctgagagcg atgaaaacct cttgaagcgc ctcaagtttg atcagagtaa actgcaagat
      841 gtactgagcg gtcttaaggc tctcattgcc atggctgatc cgctttcccg agtggatttg
      901 cgcaccaagc tcgataccgg tctggttttg gagcgagtca gttgcgccat cggggtctta
      961 gccgtcatat tcgaagcgcg tcccgatgct ctgccccaga ttgccgcgct ctctattaaa
     1021 agtggcaatg ctgccctgct caaaggcggt aaagaagcaa gccgcaccaa tcaggtgctt
     1081 tttgatttac tgaaggaaag cctgggcaca ttcgccgact gctttcacct gctttcaggg
     1141 cgagaagccg tagaggcatt gctgaaagcc cggggcgatg ttgatttgat cattccgcgt
     1201 ggctccggca gccttgtttc atacatccag agcaataccc agattccggt gcttggtcat
     1261 gctgagggtg tttgccacat ctatatagat gaaggcgctg atgcggccaa gagcctggat
     1321 gtggtgcttg atgccaaagt aagctatccg gcagcctgca atgccgtcga aacccttctc
     1381 gtgcacgatg aatttgaccg tgggcatttg cagagcatct ttgagagact ccttgcagag
     1441 aaggtagagt tgcgagtttg ccctgagctt atgaagctgt ttgccggtac cgaactgtcg
     1501 ggcaaactag tgaaggccaa gccttctgat tttggaaccg agttttccgc ccgcattctt
     1561 gcttgcaagc aagtgaaaag tcttgacgaa gccatcgacc atatcaataa atacggtagc
     1621 ggccataccg atgccatact taccgaaaat gatgatcact ggcgtcgctt tttcagccag
     1681 gtagattctg ccggtgtgta ccgcaactgt tctactcgct ttgcggacgg ctttcgttat
     1741 ggtttcggcg ccgaagtcgg
//
LOCUS       DAOQNZ010000977         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49448_length_1759_cov_2.244718, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000977 DAOQNZ010000000
VERSION     DAOQNZ010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49448_length_1759_cov_2.244718"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="PKW73_15490"
     CDS             complement(<1..1656)
                     /locus_tag="PKW73_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74751.1"
                     /translation="MNSDHDEKEANWPGRSTEEGQRLWQDALRREDWKRFGPYLSERQ
                     WGTVREDYSPDGSSWDYFDHDQARSRVYRWGEDGLLGLTDRQCRLCFSIALWNGNDPY
                     LKERLFGLTNHQGNHGEDVKEYYFYLDSTPTHSYMKALYKYPQSRYPYEELVDVNRRL
                     SRLEPEFELIDTGVMLDDNYFDVQVEYAKKTPNDIFIKITCTNRGAQPRTLHLLPQLW
                     YRNTWAWARDDDGYFAEPSISLVGDKLKASHESLGDFYFSAEQPAEVLFTDNETNMER
                     LFGVKSRSKYVKDAFHRYIVKGEKQAINPARKGTKAALHYVLLLNPYESKEFFFRLSD
                     KPEAPGSEAKAVFQERLRDADDYYNAVIPKSLSPEIKGIDRQAYASLLFSKQFYYYVV
                     KEWLEGDSNSPAPQRKHPINTNWKNIFNRDIISMPDKWEYPWYAIWDTAFHMIVMAKV
                     DPYFAKEQMVLFLREWYMRHDGQLPAYEFNFSDVNPPVHAWAAYRIYKMTAPRGERDR
                     VFLARIFQKLLINFTWWVNRKDIDGNDLFGGGFLGLDNIGLFDR"
BASE COUNT          357 a          426 c          506 g          470 t
ORIGIN      
        1 gcggtcgaag agcccgatat tgtcgagccc gaggaagccg ccgccgaaca ggtcgttgcc
       61 gtctatatct ttgcggttga cccaccaggt aaagttgatc aatagctttt gaaatatgcg
      121 ggccaggaag acgcggtcgc gctcaccccg gggcgccgtc attttgtaaa tacggtaggc
      181 ggcccaggca tgcaccggcg ggttgacgtc cgagaagttg aattcgtagg cgggaagctg
      241 cccgtcgtgg cgcatatacc attcacgcag gaagagcacc atctgctctt tggcaaagta
      301 ggggtcgacc ttggccatga cgatcatgtg aaaagccgtg tcccagatgg cataccaggg
      361 gtattcccat ttgtcgggca tggaaatgat gtcgcggttg aagatgtttt tccaattagt
      421 gttgatggga tgcttccgct ggggcgccgg gctgttgctg tcgccctcca gccattcttt
      481 caccacgtaa taatagaatt gcttgctgaa aagaagtgag gcataggctt gtctgtcgat
      541 gcctttgatt tccgggctga ggcttttcgg aatgacggca ttgtaataat cgtcggcgtc
      601 cctgagcctc tcttggaata ctgcctttgc ctcgctgcca ggggcttcag gtttgtcgct
      661 cagccggaag aagaattctt tgctctcgta aggattcaat aaaagtacat agtgcagagc
      721 ggccttggtg cctttcctgg ctggattgat ggcttgtttt tcacccttca cgatatagcg
      781 gtgaaaggcg tctttgacgt atttgctgcg gctctttacg ccgaagagcc gctccatatt
      841 ggtttcattg tcagtgaaaa gaacttcggc cggttgctct gcactgaaat agaaatcgcc
      901 gaggctttcg tggctggctt tcagtttgtc gccgacaagg gatatggagg gctcggcaaa
      961 atagccgtcg tcgtctctgg cccaggccca ggtattgcga taccagagct gcggcaaaag
     1021 gtggagagtt cttggttggg caccgcgatt ggtgcaagtg atctttataa atatatcgtt
     1081 gggagttttc ttggcatatt caacttgtac atcgaagtag ttatcgtcca gcataacgcc
     1141 ggtgtctata agttcgaatt ccggctccag gcgagatagc cggcgattga cgtccaccag
     1201 ttcttcatag gggtagcggc tctgggggta tttgtagagc gccttcatat aagagtgggt
     1261 gggagttgaa tcaaggtaaa agtagtactc ttttacgtct tcgccgtgat tgccctgatg
     1321 gttggtgaga ccgaacagtc tttctttcaa gtaggggtcg ttgccgttcc agagggcgat
     1381 gctgaagcag agcctgcatt gccggtctgt cagccctaga agtccgtctt caccccagcg
     1441 gtatacccga cttctggctt gatcgtggtc gaaataatcc caactggagc cgtccggtga
     1501 gtaatcttcc cttacagtgc cccattgtcg ttccgatagg tagggcccaa agcgtttcca
     1561 gtcttcccgg cgcaaggcat cttgccagag cctttggccc tcttccgtgc tgcgccctgg
     1621 ccagtttgcc tctttctcgt cgtggtcgga attcatggaa ttagttctaa aacttccaaa
     1681 agtgcggcgg ctttacttgg tgctgagggg cggatttacc tttagatagc gagctgcacc
     1741 gtctgaaagc aggtctcgg
//
LOCUS       DAOQNZ010000978         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49681_length_1754_cov_3.162448, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000978 DAOQNZ010000000
VERSION     DAOQNZ010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49681_length_1754_cov_3.162448"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PKW73_15495"
     CDS             <1..1085
                     /locus_tag="PKW73_15495"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA74752.1"
                     /translation="SQQRLLSEESLKSLREYKKNLLALTPKVGPQLDGQADLHISPVR
                     LGRRLVFPIPLSELIAGYVSVMIRPQDDISQLSEYMQPLALACKVDFSHRLKDSPSFA
                     VTQRSLLKDLLSGRSVSASDQERLERSFGFDLCDGFYVFAVDVGGASQGGESTGVIYP
                     DEEFISCDAEGMRAFVVPYLAKAPETWRKYADDIVARIKELNKGGGKNPAVRLGAGRL
                     VETMLELPDAYGEARQALVIGSMIHGEGEFALAYGELGVKRLLYLVFDHPELERFLDE
                     TLSPLAAYDDEWESELVDTLRVYLQQGANLNSTARALFIHRHTLRYRLEQIADILKVD
                     IDSQEVLLNLQIAFLIKDMKEGGKKK"
     gene            1085..>1754
                     /locus_tag="PKW73_15500"
     CDS             1085..>1754
                     /locus_tag="PKW73_15500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA74753.1"
                     /translation="MTVLLEAVNVVKRYDGAGTNAVNGISLQVEEGEFYSFLGPSGCG
                     KTTLLRIFAGFETLTTGSVLIGGKVMNDLPAYRRPVNMVFQSYALFPHLSVFENVAFG
                     LRHGAGRKITGLDIAAEVDKALDTVRLKALKNRMPSQLSGGQQQRVALARALAVRPLV
                     LLLDEPLSALDVQIREEMQEELSRLKRELNMTFVMVTHDQSEALALSSRVAVFNAGNL
                     EQVGS"
BASE COUNT          364 a          466 c          498 g          426 t
ORIGIN      
        1 ccagtcaaca aagattgctc tcggaagaga gtctcaagag cctcagagaa tacaagaaaa
       61 acctgctggc tctgactccg aaagtcggtc cccagcttga cggacaggct gatctgcata
      121 tttcgccggt gcgcctgggg cgccgtctgg tcttcccgat tccgctctct gagctgattg
      181 ccggttatgt atcggtgatg attcgccccc aggacgatat cagccagctt tcagagtaca
      241 tgcagcccct ggctctggcc tgcaaggtcg atttttcgca ccgcctgaaa gactcccctt
      301 cctttgccgt cactcagcgc agcctgctca aagatttgct ctccgggcgc tctgtctctg
      361 cttccgatca ggagcgcctg gagcgcagtt tcggctttga tctctgtgac ggcttctatg
      421 tttttgccgt ggatgtgggc ggcgcctctc aaggcggaga gagcaccggc gtcatttatc
      481 ccgacgaaga gttcatctca tgcgatgcgg aagggatgcg agcctttgtg gtgccctacc
      541 tggccaaagc gcccgaaacc tggcgcaaat atgccgacga catagtggct cgcatcaagg
      601 agctgaataa gggcggcggc aagaaccctg ccgttcgcct gggagccggt cgtcttgtgg
      661 aaaccatgct ggaattgccg gatgcctacg gcgaagccag gcaggccctg gtgattggct
      721 ccatgatcca cggtgaagga gaatttgccc tggcttacgg cgagctaggt gtgaagcgcc
      781 ttttatacct ggtgtttgat caccccgagc tggagcgttt tcttgatgag accttaagcc
      841 ccctggccgc ctatgatgac gaatgggagt cggagctggt ggataccctg cgtgtttatt
      901 tgcaacaggg cgccaacttg aactctactg cccgtgccct ctttattcac cgtcatactt
      961 tgcgctatcg ccttgagcaa attgccgaca ttctcaaagt cgatatcgat tcccaagaag
     1021 tcttgctgaa tctacagatc gccttcttga tcaaagacat gaaagagggc ggtaagaaga
     1081 agtagtgaca gtccttctgg aagcagtgaa tgtggtgaaa cgctatgacg gtgccggcac
     1141 aaatgccgtc aacggtattt cgctgcaggt ggaagaaggg gaattctaca gttttctggg
     1201 accaagcggt tgcggtaaga ccaccttgct tagaatcttc gccggctttg aaacccttac
     1261 caccggcagt gtcttaatcg gcggcaaggt tatgaacgac ctgccggcct acaggcgtcc
     1321 ggttaatatg gttttccaga gttatgcgct tttccctcac ctctcggtct ttgagaatgt
     1381 ggcctttggt ttgcgccatg gcgccggtcg gaagattacc gggctggaca ttgctgcgga
     1441 agtagataag gcactggata cggtgcgact gaaagctctc aaaaatagaa tgccctccca
     1501 gttgtctgga gggcagcaac agcgcgtagc acttgctcgg gctctggctg tgcgtccgct
     1561 cgttctgctc ctggatgaac ctctatcggc cctcgatgtg cagatcaggg aagagatgca
     1621 ggaagaactc tcgcgcttga agcgtgagct gaatatgacc tttgtcatgg ttactcacga
     1681 tcagtcggaa gccctggctc tttccagtcg agtggccgtc tttaatgccg gtaatttaga
     1741 gcaggtcggc tcgg
//
LOCUS       DAOQNZ010000979         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49854_length_1751_cov_3.001179, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000979 DAOQNZ010000000
VERSION     DAOQNZ010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49854_length_1751_cov_3.001179"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="PKW73_15505"
     CDS             <1..1361
                     /locus_tag="PKW73_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA74754.1"
                     /translation="ATLGYAGQQPVQFVISSVFLWTSGPHEAVMDISLRKEAQIELEG
                     FKDKLRSKFKAEMPDVSFSFEPGDLVSQIMNLGSPTPISVVVKGPDLIRCREYAERVK
                     AEMAKIPYLRDLQFGQPLDYPTVNINIDRELAGQYSLTPAQIGNALVPATSSSRYILE
                     NFWMDRKTGVAYQVQVQVPQDQIQSMEALKNFPTMISDNHEHPLLRDVASVSYGHCVG
                     EYDRDNMMRMISLTANIAGMDLGNVGKLVQDAIKRAGTPPRSVRVKLAGQAPVLSDTF
                     SHLFLGLSLAVVVIFLMLTAYFQSHKLAIVVLSTAPAIVCGVLVMLKLTGTTLNIESF
                     MGAIMSIGIGVSNAILLVNYAEMSRLSGKTAMEAALHGAQERLRPILMTSIAMVSGMI
                     PMALALSEGGQQSAPLGRAVIGGLTMSTITALTVLPLFFAVVQKHSPTTSPSIHPDDR
                     GE"
     gene            1370..>1751
                     /locus_tag="PKW73_15510"
     CDS             1370..>1751
                     /locus_tag="PKW73_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74755.1"
                     /translation="MKEAKPITLALAIGAILALSGCQKEASKETAPAAQELQEVPVVS
                     VTKKTLYRIDELPGEIQAYQDVAVYPKVPGFIDWIGVDRGSVVKKGQLLCRLIAPELK
                     AQQGEFLAKSKAVQNELQEARSRLA"
BASE COUNT          434 a          468 c          468 g          381 t
ORIGIN      
        1 aagccaccct tggctatgcc ggacagcagc cggtgcagtt cgttatcagt tcggttttcc
       61 tttggaccag cggcccccat gaggcagtca tggatataag tctgcgcaag gaagctcaaa
      121 tagaactgga agggttcaag gacaaactga gaagcaaatt caaagcggaa atgccagacg
      181 taagcttctc ctttgaacca ggagatcttg tcagccagat tatgaacctg ggctcgccca
      241 ctcctatttc ggtggtggtt aagggccccg acctgatacg ttgccgggag tacgccgaaa
      301 gggtgaaggc ggagatggct aagattccct atttgagaga tctacagttt ggccaacccc
      361 tcgattatcc caccgtcaat atcaatatcg accgcgaact ggccgggcag tacagcctta
      421 ctcccgccca aattggtaat gcactggtgc ctgccacttc aagcagtcgc tacattctgg
      481 aaaatttctg gatggacagg aaaaccggcg tagcctacca ggtgcaagtg caggtgccgc
      541 aagatcagat tcaatcgatg gaagccctca agaattttcc aactatgatt tccgacaatc
      601 acgagcaccc tctcctgcga gacgttgcct cggtttccta tgggcactgc gtcggtgagt
      661 acgaccgcga taatatgatg cgcatgattt ctctaacagc caatatcgcc ggtatggatc
      721 tgggcaatgt cggcaaactg gtgcaggatg ccattaagag agccggcacg ccgcctcgca
      781 gtgtacgcgt caaactggca ggtcaggcac cggtcctgag cgataccttt tcacacctct
      841 tcctgggtct gtctttagcc gtagtggtta ttttcctgat gctgacagcc tactttcagt
      901 cgcacaagtt ggccatagta gtgctgtcca ctgctcccgc catagtttgc ggcgtcctgg
      961 tgatgcttaa actgacgggt accacactca atatcgaatc tttcatgggc gccattatgt
     1021 ccatcggtat cggcgtcagc aacgccatcc tactggtcaa ctatgcggag atgagccggc
     1081 tgagcggcaa aacagctatg gaagcggctc tacacggagc ccaggaacgg ctccgaccga
     1141 tcctcatgac ctccatagct atggtatcag gaatgatacc aatggctcta gccctgagtg
     1201 agggaggaca gcaaagcgca ccgctgggcc gggctgttat cggcgggctg accatgtcca
     1261 ccatcacggc cctgacggtg ctgcctctct tctttgccgt agtgcaaaaa cacagcccca
     1321 ccacatcacc ctcgatccac cccgacgaca gaggcgaata ggaacacgta tgaaagaggc
     1381 aaaaccaatt actctggctc tggcaatagg tgctattttg gctcttagcg gctgtcaaaa
     1441 ggaagcgagc aaagagacgg caccggcggc gcaagaatta caggaagtgc cggtggtcag
     1501 cgtcacaaaa aaaaccttgt atcgcatcga tgaattgccg ggtgaaattc aagcctacca
     1561 ggatgttgcc gtctacccga aagtgccggg cttcatagat tggatcggtg tggaccgggg
     1621 ctcggtggta aagaaaggac aacttctctg cagactgatc gctccggaat tgaaagctca
     1681 acaaggtgag tttctggcca agtctaaggc tgtgcagaat gaattgcagg aagcccgttc
     1741 aagactggca g
//
LOCUS       DAOQNZ010000980         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_49913_length_1750_cov_3.860177, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000980 DAOQNZ010000000
VERSION     DAOQNZ010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49913_length_1750_cov_3.860177"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PKW73_15515"
     CDS             complement(<1..735)
                     /locus_tag="PKW73_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74756.1"
                     /translation="MSVSQSLKYKSRQNQIGYWCGVLLFPLWGVVMPALFIINLCSAM
                     VLLVSSPTTSLIPFGAGFYCAAAFLLGAIFTGVMKDNEILIDNSGIAIPFLLSLKHKR
                     RRYLPWADVEAVLVETDGQASAAGQKQKVVIKSADGEIDLALANFKNMDREQLLISLS
                     LFAPETAVDNSLLSARDALGQEKEDGEAGANLHLPSFTSIWEEELVSRFSSTAYVPLY
                     PGQSINDGRYTVIRQLNLGGWSAVYLC"
     gene            complement(732..>1750)
                     /locus_tag="PKW73_15520"
     CDS             complement(732..>1750)
                     /locus_tag="PKW73_15520"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74757.1"
                     /translation="GGSLGAGGEGVTYLVEDRQTKHKMVLKEVILPSHAGQVMKERSL
                     RNFQNQARLLSQIDHEGIARLKEHFVRQGRAYLVLEYIEGESLAQLVDANGPLTARRT
                     AELALAMAGILEHLHSLKPPVLHRDFTPHNLIIEPGGKLKLIDFGVALEDKDLINQEK
                     ASMVGKQSYMPLEQIRGKGCTASDIYAFGCCLYFMLSGSDPEPLKSAVLPSNGDGSKK
                     RTLSETLGQIIEKCTRQKVADRFQTAAEIVALLEEALKEDEQENLSVTIGTSTLEADE
                     SRTEASLFSKIKLVVVNSSSDSEKKLSGQDPDKNAKKAAKKEPKKDSKVGKKGKKGRK
                     NKRA"
BASE COUNT          353 a          449 c          477 g          471 t
ORIGIN      
        1 gcaaagataa acggccgacc aaccgcccag gttgagttgc cgtatgaccg tatagcgacc
       61 gtcgtttatg ctttgccctg gatagagggg cacataggct gtggacgaaa accggctcac
      121 caactcctct tcccagatgc tggtaaagct tggtagatgc aggtttgctc ctgcttcgcc
      181 atcctctttc tcttgcccga gggcgtcgcg ggctgaaagc agggaattgt ccacggcagt
      241 ttccggtgca aagagcgaga gggagatgag cagttgctct ctgtccatgt ttttgaagtt
      301 ggccagggcg agatcgattt caccgtcggc agatttaatc accaccttct gtttttgccc
      361 tgcggcactt gcttgcccat cggtctcaac cagcaccgcc tctacatcgg cccagggtaa
      421 ataacgcctg cgtttgtgct tgaggctgag cagaaaggga atggctatgc ctgagttgtc
      481 gatgagaatc tcattgtctt tcatgacacc ggtgaagatg gctcccagaa gaaaagcggc
      541 ggcacagtag aagccggctc cgaaaggaat cagggatgta gttgggctgc ttaccagaag
      601 taccatggcc gagcagagat tgatgataaa gagagccggc atgaccacgc cccagagtgg
      661 aaagaggagc actccgcacc agtagccaat ctggttttgt cggcttttgt atttgagact
      721 ctgggagacg ctcatgccct cttgttcttt ctcccctttt tgcctttctt gccgaccttg
      781 ctatctttct tgggctcttt cttagcagcc ttcttagcat tcttgtcagg atcttgcccg
      841 cttagctttt tctcgctatc ggaggaggaa tttactacaa caagctttat tttgctgaaa
      901 agcgatgctt ccgtgcggct ttcgtcagct tccagggtag aggtgccaat agtgacagac
      961 agattttcct gttcatcttc tttgagagct tcctcaagta atgcgactat ctctgcggca
     1021 gtctggaagc gatctgctac cttttggcgg gtgcattttt caatgatttg tcccagtgtt
     1081 tcagaaaggg ttctcttctt cgacccgtcg ccattcgagg gcagaactgc gcttttgagg
     1141 ggctctgggt ctgagccgga aagcataaag tagagacagc agccgaaggc atagatatca
     1201 ctggcggtgc agcctttacc tcggatctgc tccaggggca tatagctttg cttgcctacc
     1261 atagaggctt tctcttgatt gattagatct ttgtcttcca gggcgacgcc gaaatcaatc
     1321 aatttcaact tgccgcctgg ttcgatgatc aggttgtggg gtgtgaagtc tcggtgcaga
     1381 acgggcggtt tgaggctgtg cagatgttcg agaatgccgg ccatggcaag agccagctcc
     1441 gctgttcggc gggcggtgag aggtccattt gcatccacca gttgggccag actttctcct
     1501 tcgatgtact ccagcaccag gtaggcgcgg ccttgtctga caaaatgttc cttcaggcgg
     1561 gcgatgccct catggtcaat ctgactgagc agacgggctt gattttggaa gtttcttagg
     1621 ctgcgctctt tcatcacctg tccggcatgg gaaggcaata tgacttcctt taagaccatt
     1681 ttgtgctttg tctgccggtc ttccaccagg taggtgacgc cttcgccacc ggcgcccagg
     1741 ctgcctccac
//
LOCUS       DAOQNZ010000981         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50144_length_1746_cov_4.010053, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000981 DAOQNZ010000000
VERSION     DAOQNZ010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50144_length_1746_cov_4.010053"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKW73_15525"
     CDS             <1..294
                     /locus_tag="PKW73_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA74758.1"
                     /translation="LLKNHAGRIVEAFGLKRVEADIITDESPANGILTLANDWQADLV
                     VTGSRGANRNPDFRAGNTAVGILDRVHCSVIAVQMDAKKQVKFHWSQSEAAKK"
     gene            complement(305..1381)
                     /locus_tag="PKW73_15530"
     CDS             complement(305..1381)
                     /locus_tag="PKW73_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74759.1"
                     /translation="MQYLSRAFAGVETKIMSRAKPIRIGKNLNRVINPQLKRIGILTL
                     GLLSLTVVMAGQAQAASPTYSKYDWGEPEFKAKPRHSIQFPREKALGGLAISDKPIFQ
                     HGEAKTKNLLAQGQIDIPAGKFVIYFPNHVFFHNPQLIETLKPDSIDSVVLRYATMED
                     GEEGYSVKAMPYLAKLTDIKVMDLEKSEMNDSGLSALKPLKNLQALLLFANEFDGSFL
                     KELTGLNKLKVLILNNNDVKVENLRYLSRYKSIKVLGLSYTHLTAASAGFLADMPQLE
                     TLLLAGNYQLDDKVIPILAKLKNLHFLDIRKTGITMNGFKRLKVLLPKTMVHSYLDNT
                     SPNAKPKNSEVETIFAPLSRGRKL"
BASE COUNT          383 a          398 c          492 g          473 t
ORIGIN      
        1 ctgcttaaga accatgccgg ccgcattgtg gaggcttttg gtttgaagcg ggtggaggcc
       61 gatatcatca ccgatgaatc gcctgccaat ggaattctca ctctcgctaa cgactggcag
      121 gccgatctgg ttgtgaccgg ctctcgcggc gccaatcgca atcccgattt tcgggccggc
      181 aatacggccg tgggaatatt ggaccgcgtg cattgttcag tgattgccgt gcagatggat
      241 gccaaaaaac aggtcaaatt tcactggagc cagtcggaag cggctaagaa atagcaggtg
      301 atgcttagag cttgcgtccc ctggagagcg gtgcgaagat agtttctact tcgctgtttt
      361 tcggctttgc attcgggctt gtgttatcca gataggaatg aaccatggtt ttggggagca
      421 aaaccttcaa gcgcttgaag ccgttcatgg tgataccggt tttcctgatg tccagaaagt
      481 gtaaattctt cagcttggcc agaatgggga taactttgtc gtccaattga taattgccag
      541 ctaaaagcag ggtttcaagc tggggcatgt cggcaagaaa accggcggag gcggcagtga
      601 ggtgagtgta ggatagcccc agcactttga tgcttttgta gcggctcaag tagcgaagat
      661 tctccacttt cacgtcgttg ttgttcaaaa ttagtacttt tagtttattt agaccagtga
      721 gttccttcaa gaaagagccg tcgaattcat tggcgaagag caaaagagcc tgcaggttct
      781 tcaagggctt gagcgcagac agcccggagt cgttcatttc cgatttttcc agatccatta
      841 ccttgatgtc ggtaagtttg gctaaatatg gcatggcttt gaccgagtaa ccttcttcgc
      901 catcttccat ggtggcatag cgaagcacca ccgaatcgat gctgtccggc ttcaatgtct
      961 ctattaactg tgggttgtgg aagaaaacat ggttcggaaa ataaatgaca aatttgcctg
     1021 ctggtatgtc gatctggccc tgcgccagaa gatttttggt tttggcttcg ccgtgctgaa
     1081 aaataggttt gtcggatatg gccaggcctc ccagggcttt ctccctcgga aactgtatag
     1141 agtgtctggg tttggctttg aattcgggct cgccccagtc atatttgctg taggtcggtg
     1201 acgcagcctg agcctgaccg gccatgacta cggtgaggct aagcaggccc aacgtgagta
     1261 tgccgatgcg cttcagctga gggtttatga ctctgtttag atttttaccg atacgaattg
     1321 gtttggccct gctcataatc ttggtttcga cgcccgcaaa ggctctactc aggtactgca
     1381 ataggaaaac ttgcagccat ccaacaatta tctcatctcc tggtgctctg gttgcttata
     1441 tgagcctgat cttggcccgg tctggagaat cgggcgacgg gcttcgactg atggcaaagg
     1501 agggcaggga aggcaaggct ggaaaggaag gggcggtcag atggctgggt atattaatag
     1561 ggttgcttca atctgagcgc taccccccac ggtaagaaaa ttgaacatgt ctgcttccat
     1621 taagggcaag aggcctgctt ggggcgcctt ctttcgggag gtggccgcgg ctctatcttt
     1681 ctgtcttctt ctggggcttt ttcttcccac ccgggcagct gaaaagactg tgccggtttg
     1741 gcttct
//
LOCUS       DAOQNZ010000982         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50158_length_1746_cov_3.441159, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000982 DAOQNZ010000000
VERSION     DAOQNZ010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50158_length_1746_cov_3.441159"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PKW73_15535"
     CDS             <1..1136
                     /locus_tag="PKW73_15535"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNA74760.1"
                     /translation="MFELLLNCPPYREYLQGRDNLQEIMIGYSDSGKNGGIVASNWEL
                     HKAQKALVETARRYDVKLRLFHGRGGTIGRGGGPTHRAILAQPAGTVDGRIKITEQGE
                     VISSKYAMHGIAVRNFDRLAAAVIKASLKSKDRPEKASWLEFMEELSALSFKAYRNLV
                     YESPGFVDFFCQTTPITEISELKMGSRPTRRTAGSSAIEDLRAIPWVFAWTQSRYMLP
                     AWYGFGQAMKELLDKPGNLSLCREMYREWPFFAGLVSKIETALAVSDLDIARHYAINL
                     VEPELYKRFMPRIETEFAQCREAVLSIAEAGSLLATIPYLAHSISLRNPYVDPLSYLQ
                     VKLIKEFRQACRENSSSIEEKASLLEAVLMTINGVAEGLQNTG"
     gene            1149..>1746
                     /gene="proB"
                     /locus_tag="PKW73_15540"
     CDS             1149..>1746
                     /gene="proB"
                     /locus_tag="PKW73_15540"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNA74761.1"
                     /translation="MDRESLIKDASRIVIKLGTAVLMKDEGGIALSRFYSFVESIAEL
                     MKAGKEVLLVTSGAVGLGVRTLNLPGKPKDLPLKQACAAVGQGRLMSLYNDAFEKADV
                     VAAQVLLTEEDFSNRKRYLNLRSTISELLALKVLPIINENDTVSTAELELLKEEGVKV
                     NFGDNDKLSALVASKMDADLLIILTDVEGLYTADPRLGP"
BASE COUNT          414 a          494 c          469 g          369 t
ORIGIN      
        1 ccatgttcga gcttctcttg aattgcccgc cttacagaga gtacctgcag gggcgtgata
       61 atctgcagga gatcatgatt ggctattccg atagcggcaa aaacggcggt atcgtggcct
      121 ccaactggga actgcacaaa gcccagaaag ctctagtgga aacagcccgt cgctatgacg
      181 tcaagctgag gctcttccac ggacggggcg gcaccatcgg ccgtggcggc ggccccaccc
      241 accgagccat tctggcccaa ccggcaggca ccgtggatgg tcgcatcaaa attaccgagc
      301 agggtgaagt aatctcttcc aagtatgcca tgcacggtat cgccgtacgc aacttcgacc
      361 gtctggccgc cgccgtcatc aaagccagcc tcaagagcaa agaccgcccc gaaaaggcat
      421 cctggctgga gtttatggaa gagctttctg ccctgtcgtt caaggcttat cgtaatctgg
      481 tgtatgagag cccaggcttt gtggacttct tctgccagac cacgcctata acggaaattt
      541 ccgaacttaa aatgggctcc cgccccacac gccgcaccgc cggcagtagt gccatcgaag
      601 acctgcgcgc cataccgtgg gtcttcgcct ggacccagag tcgctatatg ctaccggcct
      661 ggtacggctt cggtcaggcc atgaaggaac ttctcgataa acctgggaac ctctcacttt
      721 gcagggaaat gtacagagag tggcccttct tcgccggact ggtaagcaaa atcgagactg
      781 ccctggctgt ttccgatctc gacatcgccc ggcactatgc cataaacctt gtggagcccg
      841 aactctacaa gcgcttcatg ccccgcatcg aaaccgaatt cgctcaatgt cgcgaggcgg
      901 tgttgagcat tgccgaagcc ggaagtttgc ttgccacaat tccttacctg gctcactcta
      961 tttccctgcg caacccctat gtggatcccc tcagctatct gcaggtaaaa ctaatcaagg
     1021 aattcaggca agcctgccgg gaaaactcca gttccatcga ggaaaaagca agcctgctgg
     1081 aggcggtttt gatgaccatt aacggcgtgg ctgaaggttt gcagaatacg ggttaggaaa
     1141 caggtgtaat ggatagagaa agcctaataa aggacgcttc gcgcattgtc attaaactgg
     1201 gcacagccgt cttgatgaaa gacgaagggg gcatcgccct cagtcgcttc tacagcttcg
     1261 ttgaatcgat tgccgaactg atgaaagccg gcaaggaagt acttctagtt acctccggcg
     1321 ccgtcggtct gggggtgcgc acccttaact tgcccggcaa gcctaaagat ttgccgctca
     1381 agcaggcctg cgcagcggtg ggccaggggc ggcttatgag cctctataac gacgcctttg
     1441 agaaagccga cgtggtagcc gcccaggtgc tcctcaccga ggaagatttc tcgaaccgca
     1501 agcgctattt gaacttgcgc agcaccataa gcgagcttct ggctttgaaa gtcttgccta
     1561 taatcaacga gaatgacacc gtttcaactg ctgaactgga gcttctcaaa gaagaagggg
     1621 tgaaggtcaa cttcggcgac aacgacaaac tctctgccct tgtggcaagt aagatggacg
     1681 ccgacctgtt gattatcctg accgatgtgg aagggcttta tacagccgat ccgcgccttg
     1741 gaccgg
//
LOCUS       DAOQNZ010000983         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50203_length_1745_cov_3.797041, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000983 DAOQNZ010000000
VERSION     DAOQNZ010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50203_length_1745_cov_3.797041"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1643
                     /locus_tag="PKW73_15545"
     CDS             <1..1643
                     /locus_tag="PKW73_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74762.1"
                     /translation="QEKMQLLDAWRGISEQIVAFNGKQGGGHLQNGVYRYTQETLVKL
                     GLDKQGWEALPAQTGSTLDMIGGDVLLVNRRTGQMELIDASSRRLDPTTGAPLSSSES
                     QKANVPLLREQGVIDALPRWFDLSGKVEFDQDSPEQSRRVKEFAEDFSHRIKELTRAD
                     SHGPFNLRDFPLPSPTVSRDKEATSREIKAVVDWSQKNADLSWRSGERNLSANYREFG
                     RVIETGALAFSSRVQSTSLTDAVGKLAERIILEDAAKAIYPVAKSGAESLPSIAHRQV
                     SQEGTVVQVKPDGALVVSLRQSGNGQPEIYSSPSVLGAFDNASKKLALAATRPEQHAE
                     LAKELPAHYRHMFEAGKLDMGKILQVVARFRNQFAAGGVGTERALLGHLVHRLVERKP
                     DELRRLATGAGNQTTRGGDAVTREVASKEAAPKLEQIGRKGDGGVNDARFSDHLSPKE
                     LARLAELQSTLEGKGNLSPSEKETLRSLRQAQEELAKPGAKTPQLNSRLAAVRRVIGL
                     EGVNKVAGPAMLAAVVLNLYRASASEVDEAQPTFGMGW"
BASE COUNT          397 a          452 c          535 g          361 t
ORIGIN      
        1 cccaggagaa gatgcagctt cttgatgcct ggcgaggcat ttcagaacaa atagtggcat
       61 ttaacggcaa gcagggtggc gggcacctgc aaaatggtgt ttatcgctac acccaggaga
      121 ctctggttaa gcttgggctc gataaacagg gttgggaagc cctgcctgct caaacaggca
      181 gcaccctgga tatgatcggc ggcgatgtgc ttctggtgaa caggcgcacc ggtcagatgg
      241 aactgataga tgcttccagt cgacgcctcg accccactac cggggcaccg ctttcgagca
      301 gcgagagtca gaaagccaat gtgccattgc tgcgtgaaca gggggtgatt gatgctctgc
      361 cccgctggtt tgatctgagc ggcaaggtgg aattcgatca ggattctccg gagcagtcca
      421 ggcgggttaa ggaatttgcc gaagacttca gccaccgcat caaagagctg acccgggctg
      481 atagtcatgg accattcaat ctgcgagatt ttccgctacc gtctcccacg gtcagtcgag
      541 ataaagaagc cacctcccgc gagatcaagg ctgtagtaga ttggtcccag aagaacgccg
      601 atctaagttg gcgctcggga gaacgtaatc tctcggccaa ttatcgtgag ttcgggcgcg
      661 tcatcgaaac gggtgctctg gcattttctt cgagagtgca gtccacttcc ctcaccgatg
      721 ccgttggaaa attggccgag cgcataattt tagaagatgc cgccaaagcc atctatccgg
      781 tggctaaaag tggtgctgaa agtttgcctt ccatagcgca ccggcaggtg agccaagagg
      841 gcacggtggt gcaggttaag cccgatggtg ccctggttgt ctcattgcgt caaagcggca
      901 atggtcagcc tgaaatatac agttcgccct cggtgcttgg tgcctttgac aatgcttcca
      961 agaagctggc tctggcagct accaggcctg aacaacatgc agagctggcc aaggaattgc
     1021 cggcccatta caggcatatg ttcgaagccg gcaaactcga tatgggcaag attctgcaag
     1081 tggtggcccg ctttcgcaat cagtttgccg ccggcggtgt gggtactgag cgtgccctgc
     1141 tcgggcatct ggtgcaccga ctggtggagc gcaaacccga tgagctgaga cgactggcca
     1201 ctggtgccgg aaatcagact accaggggcg gtgatgccgt caccagagaa gtggctagca
     1261 aggaagcggc tcccaaactt gaacaaatcg gtcgcaaggg cgatggcggt gtcaatgatg
     1321 cgcgcttcag cgatcatctc agccccaagg aattggctcg actggctgaa ctgcagtcta
     1381 ccctggaagg caagggaaac ctgtctccat cggaaaaaga gacgcttcgc tctttgcgtc
     1441 aggcccagga agaactggcc aaacctggtg ccaagacgcc gcagttgaat tctcgcctgg
     1501 ctgccgtgcg gcgggtaatc ggtctggaag gcgtgaataa ggtggctgga ccggctatgc
     1561 tggcggcggt tgtcctcaat ctttatcgcg cttccgccag tgaagttgat gaagcgcagc
     1621 cgaccttcgg catgggttgg tagcgatggc cggtgatggt gcagggaacg ataagcagcc
     1681 gccagcattt ccgcttatgg ggcgggagtc tgtccaaaag gtcgagcaag atactgatag
     1741 caagc
//
LOCUS       DAOQNZ010000984         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50213_length_1745_cov_3.186982, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000984 DAOQNZ010000000
VERSION     DAOQNZ010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50213_length_1745_cov_3.186982"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..506)
                     /locus_tag="PKW73_15550"
     CDS             complement(111..506)
                     /locus_tag="PKW73_15550"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNA74763.1"
                     /translation="MNKLRFLFILSAFFLAPLAAFADNTVSSTTPPTDPKLYGAYLAK
                     RIKRHWFPVQNAGDLTTVIKFTIQPDGTLKDLSVARSSGNHVVDAAARRAVESSAPFG
                     PLPAGLDSKPLRVTKVFNLTQVNNVQLGD"
     gene            complement(609..1502)
                     /locus_tag="PKW73_15555"
     CDS             complement(609..1502)
                     /locus_tag="PKW73_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74764.1"
                     /translation="MSPQRSPLRIHVLLAKESSEALVIKRGPTKQVQLVRWQRDKDTF
                     IAGQWFKGRIYERRCDLSPSGKYFLYFAASHKPPLYSWTAVSRPPYFSALALWPKGDC
                     WGGGGLFESDKKILLNHGQCQMELDKQFSLPKQLKISTLEGSAGGGEDFPIYHLRLLR
                     YGWTLEAAGESDLRKKMLVTSAYEIPLLYSKWCHSNAGTKANTDTTQRYALKMFIRSV
                     AEQNEPWYIIEYQVENQNGEALKDLGRLDWADWDSNGDLLFAKEGKIFRAKDIDDPIE
                     LIDLTANIFEEMPPEPWAMNW"
BASE COUNT          362 a          419 c          482 g          482 t
ORIGIN      
        1 cgggtatctc ggagatttca gcctttctcg ccagctctgg ctgagaccgg ctgagaagaa
       61 gcggcttttg ctctcaccca cagtgtacgc cggctatgcg tttggtgttc ctagtcaccg
      121 agctgaacat tgttgacttg agttagattg aagaccttgg tgacgcgcag tggcttgcta
      181 tctagacctg ccggcagggg accaaatggg gccgagcttt cgaccgcgcg ccgcgctgcc
      241 gcatcgacaa cgtgattgcc ggaagatctg gctacggaaa ggtctttcag tgtgccgtca
      301 ggctgtatgg tgaatttgat tacagttgta agatcgcctg cgttttgaac tggaaaccag
      361 tgccttttga tgcgcttagc caggtaggcg ccatagagct tgggatcagt aggaggcgtt
      421 gtactggaga ctgtgttatc ggcaaaggct gctaagggtg caaggaagaa ggctgaaagg
      481 ataaatagaa aacggagctt attcatagag caccttctgc tggagagccc agtcagtata
      541 ccgcactggt tgcgcaggtt tctaccgaaa tggtgaggct ggtgtgcctg ctcggcgggt
      601 gatgatttct accagttcat ggcccatggt tctggaggca tctcttcaaa gatgttggcg
      661 gttagatcaa tcagttctat cgggtcgtca atatctttag ctcggaatat cttgccctct
      721 ttggcaaata gaagatcgcc gttactatcc cagtctgccc aatcaaggcg tccgagatct
      781 tttaatgctt caccattctg attttcaact tgatattcga ttatgtacca gggttcgttt
      841 tgctcggcaa cgctgcggat aaacatcttc agtgcgtatc tttgggtggt gtctgtgttc
      901 gctttggtgc ccgcattgct atggcaccac ttggaatata gaagcggtat ttcgtatgca
      961 gaggtcacca gcattttttt gcgcaggtct gattctcctg ctgcctccag ggtccagccg
     1021 taacgtagca gtcttaaatg atagatcgga aaatcttcgc cccctcctgc tgagccttcc
     1081 agggttgaga tcttgagctg tttaggtaag gaaaactgtt tgtccagttc catctggcac
     1141 tgcccgtgat tgagaaggat cttcttgtcg ctctcaaata gtccaccgcc gccccagcaa
     1201 tcgccctttg gccagagtgc cagggctgag aaatatggtg gtcggctgac agccgtccat
     1261 gaatagaggg gaggtttgtg gctggcggca aaatagagga agtacttgcc ggatggtgac
     1321 agatcgcatc ggcgctcata gattcggccc ttgaaccact gaccggcaat gaaggtgtct
     1381 ttatctcttt gccatcttac cagttgtact tgcttagttg gtccgcgttt tatcaccagc
     1441 gcttcgcttg attctttagc cagcaagacg tgtatgcgca gaggagatcg ctgagggctc
     1501 atattttcga gggctctgaa tttaaggtac ttgtagctgg gctattctac aatgcttccc
     1561 tgcttgattc agcgctaggt tgtgaagcat atgaaaaggc tggttcccat gaccgctccc
     1621 tcatcctgcc gttcgttgtc agcggctctg gcgcttgtgc tgggtacctt gtgtgggtta
     1681 cttttagcca tgcctgcggc tcagacccgc gagaattccc ggcagatctg gcacctcaaa
     1741 cagaa
//
LOCUS       DAOQNZ010000985         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50274_length_1744_cov_2.946122, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000985 DAOQNZ010000000
VERSION     DAOQNZ010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50274_length_1744_cov_2.946122"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..333
                     /locus_tag="PKW73_15560"
     CDS             109..333
                     /locus_tag="PKW73_15560"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNA74765.1"
                     /translation="MAYTIVADLCEGIADCIPVCPVECIHWAEGKTNTKGTKYTYIDD
                     STCIDCGACLSVCPIEGAILDEWKPELQKP"
     gene            complement(346..1089)
                     /locus_tag="PKW73_15565"
     CDS             complement(346..1089)
                     /locus_tag="PKW73_15565"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HNA74766.1"
                     /translation="MKKLRLRDLFLKFPLLDKWRVLGLARLFTHGITFFDRRYRVSAI
                     IPDESKPYVQTVFAHCPPGSLDSARPAIFAYYHGQMFILLGLTPRYKMTVLASNSRDG
                     EMIARAAEGMGFPVARGSRTEGGTKGARLLLKAAREKRSLLFPVDGPRGPAMKIKEEI
                     LRMAQLSKLPIVPVVAAIATLQKTKSWDSYNCPHIFTSIVYIFGKPVFVPHMDNEKVE
                     SLRLDLETYMSQLKSKADQFFEVTAKNLY"
     gene            1287..1556
                     /gene="rpsO"
                     /locus_tag="PKW73_15570"
     CDS             1287..1556
                     /gene="rpsO"
                     /locus_tag="PKW73_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNA74767.1"
                     /translation="MALLAERKQEIISTNQTHAKDTGSPEVQVAMLTDRISQLTEHLK
                     SHSKDFHSRRGLLMMVGKRRRLLQYLMKEDNERYRKLIEKLGLRR"
BASE COUNT          465 a          403 c          451 g          425 t
ORIGIN      
        1 cggacaataa ctttgaagct gaagttggtt agctgtccct ttccaattac agttgaaaag
       61 aattccgtag gaccgactta ctaaaagata tctgtaagga gtttgctgat ggcttacaca
      121 attgttgctg atctatgcga aggcatagct gattgcattc cggtttgtcc cgtagagtgc
      181 attcactggg ctgaaggtaa aacaaatacc aaaggcacca aatatacata tatagacgac
      241 agcacctgca ttgattgcgg cgcttgcttg tccgtatgcc ctatcgaagg cgccattctt
      301 gatgaatgga agcctgaact gcagaaaccc taatttcttc gtaagttagt aaaggttttt
      361 tgccgttact tcaaagaact ggtcggcctt cgacttgagt tgagacatat aagtctcaag
      421 atcgagtcga aggctttcca ctttttcatt gtccatgtgg ggcacaaaaa ccggcttgcc
      481 aaaaatgtaa acaatagacg taaagatgtg gggacagttg tagctgtccc atgatttggt
      541 cttttgcagg gtggcaatag ctgctactac gggaactatg gggagcttgg aaagttgggc
      601 catccgaaga atttcttctt tgatcttcat ggccggaccg cgcggaccat ccacaggaaa
      661 gagaagactg cgtttttccc tggccgcttt gagcaggagc cgggcgcctt tggtgccgcc
      721 ttcagtgcgg gaaccgcgcg ccactgggaa acccatacct tcggcagcac gggctatcat
      781 ctcgccgtcc cgggagttac tggccagcac cgtcattttg taacgaggtg taagcccgag
      841 caagataaac atttgtccat gatagtaggc gaaaattgcc gggcgggcgc tgtcaagtga
      901 gcccggcggg cagtgggcga aaacggtctg cacataaggc ttgctctcgt cggggatgat
      961 ggccgacacc cgatagcggc ggtcgaagaa ggtgattccg tgggtgaaaa ggcgcgccaa
     1021 tccaagaaca cgccacttat caagaagggg aaatttcagg aagaggtctc ggagtcgcag
     1081 ttttttcaag gttctggctg attttgtgaa tgatgtaaac agtatggtcg aaacaggtga
     1141 atgccctgaa aatagaccaa agattggctg aattttagaa atcaagtggc tccaacagga
     1201 gcagccatca ggacggcagg cattttgtga tattatctac caacgccgaa tatcgtaaga
     1261 atatcaataa ccgacggaaa gaagtaatgg cacttttagc agaacgcaag caagaaataa
     1321 tttcaacaaa ccagacccac gccaaagata ccggttcgcc ggaagttcag gtggcgatgc
     1381 tcaccgatcg cattagccaa ttgaccgagc acttgaagtc tcattccaaa gacttccaca
     1441 gccgccgtgg tcttttgatg atggtcggaa agcgacgcag acttcttcaa tatttgatga
     1501 aggaagacaa tgagcgttac cgcaagctta tcgagaagct cggactgaga cgctagttaa
     1561 tcgttgaaaa gggcgggctt aagctcgcct tttttattta tggcacccag ggtgtcgaca
     1621 tcattctcta ccgcagcctc tccacttccg gtaatttctt gattaccccg gggcgctgcc
     1681 gtctttcaag accagccaga cctttcatag acccagacct ttctatacaa ggtatggtgc
     1741 tggc
//
LOCUS       DAOQNZ010000986         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50315_length_1743_cov_4.625592, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000986 DAOQNZ010000000
VERSION     DAOQNZ010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50315_length_1743_cov_4.625592"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1565
                     /locus_tag="PKW73_15575"
     CDS             177..1565
                     /locus_tag="PKW73_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74768.1"
                     /translation="MQQKPSELVPSALSLLSVWEKLWIVVVCFAYLALALAAAHLLSS
                     TPFLTEPRGMAAVSIYGGVLVGFGFAAVVSWSSLPRLIDRLVDSGMLSSAERLSRIYA
                     SLLQVLSPLTKDTALAVAVQADIERARGNYDTAVVLAYQALLILRRHDVLMAGSPGNE
                     EEDSLVKALAESEQDNITGAICRRILSNILCDLGNCEQALKEILASISDLEQSIQAIQ
                     KPMLLSSGPAATGAGSGSGAGAGTGAGDGDGAGAGAGVGAGANASVCTGDGKAAAVLV
                     RHAQKKSRSQLSANLKFARSVLSDALSVAGRAYNLLERYDEAERFLERASQLKQELAA
                     DDLSLQEVRAQLALTFYLSGRLSDCQSLVQQGLSLLTGKASRAAGLVRSDLLVLEAKV
                     LLPSKPIEAKVQLQEALKIRRKWLGAASLEIAEVLESLTVCFDQTGESDSASKAREDA
                     ARIKARIKTRQV"
BASE COUNT          379 a          397 c          470 g          497 t
ORIGIN      
        1 catctcagtt ccagtacata ttaccaatct gtgacggaag tcgtatcatc cgttgtctcc
       61 ttcagcttaa ctgttctcca cccgcagaac ctggttcagc ttataatggc ttgattctag
      121 cttggccgat attagtaaag cctcaatctg gatcggtaag atcattaagt agtcttatgc
      181 agcaaaaacc ttccgaactt gtgccttccg ctctttccct cctgagcgtc tgggaaaaac
      241 tgtggatagt ggtggtctgt tttgcatatt tggctcttgc tctagccgct gcccatttac
      301 tttccagtac gccatttttg accgaaccca ggggtatggc tgctgtttct atttatggcg
      361 gagtgcttgt aggattcggt tttgcggcgg tggtaagttg gtcctctttg ccgcgcctga
      421 ttgatcgcct tgtagatagc ggcatgctgt cctccgccga gagattgagt cgcatctatg
      481 cttccctttt gcaggttctt tctcccctga ctaaagatac ggccctggct gtggcagttc
      541 aagctgatat cgagcgtgct cgcggcaact atgatactgc cgtagttctg gcttaccagg
      601 ctttactgat tcttcgccgg catgatgtat tgatggcagg gtcgcccggt aatgaggaag
      661 aggattccct agttaaagcg ctggccgaat ctgaacagga caacataacc ggagccatct
      721 gccgccgcat cctctctaac attctttgcg atctgggcaa ttgcgaacag gcattgaagg
      781 agattctagc ttcgataagc gatttagagc agtccatcca ggcaattcag aagcccatgc
      841 tgctctcatc cggtcctgcc gcaactggag ctggatctgg ttctggagct ggcgcaggaa
      901 ctggagctgg tgatggtgat ggtgccggtg ccggtgctgg tgtcggtgct ggtgctaatg
      961 ctagtgtttg tactggtgat ggtaaggctg cggctgttct ggtcagacat gcgcaaaaga
     1021 aaagccggag ccagttgagt gcaaacctta agttcgccag gtcggtgctc tctgatgcgc
     1081 tttctgtggc cgggcgtgcc tacaatctac ttgaacggta tgatgaggct gagcgatttc
     1141 tagagagagc atctcagtta aagcaggaac ttgctgctga tgatttgtct cttcaggaag
     1201 taagggccca gttggcgctt actttttatt tgagtgggcg tctttcagac tgtcaatctc
     1261 tggtgcagca aggcttgtct ttgcttacgg ggaaggcgag tcgtgccgcc ggtttggtgc
     1321 gcagcgatct acttgttctt gaggctaaag tgttgcttcc ctccaagccg atagaggcga
     1381 aagtgcagtt acaagaagcc ttaaagatac gtcgaaaatg gcttggtgct gctagtttgg
     1441 aaattgctga agtattagaa tctttgactg tctgttttga tcagaccggc gaaagtgatt
     1501 ccgcttcaaa ggcgcgcgaa gacgcggctc gaattaaagc cagaatcaag accaggcaag
     1561 tctagattgg aagaagacaa ggcaactcta tagtttaatc gagtatttgc tgccaatatc
     1621 taatgcgagt gctcgccttt acaacgttct gagagtgctg ccaaagtcgg cttgggtagt
     1681 cattttgcgg aatcgctgca attgtaacaa tagtgatttt gcgaaatatt acatcagcat
     1741 ttc
//
LOCUS       DAOQNZ010000987         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50454_length_1741_cov_2.976868, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000987 DAOQNZ010000000
VERSION     DAOQNZ010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50454_length_1741_cov_2.976868"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKW73_15580"
     CDS             complement(<1..373)
                     /locus_tag="PKW73_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74769.1"
                     /translation="MSTEDPKLEQSLSAPDRHLKLTLGILILLYFALRLPWLFMVPMV
                     GAPDEFSHFWVLKFMAEYLRLPEAAEVAAGGPSAVYGSLPQIGYLPHVFTARLLPLMP
                     LPGLSGCDLSVTARFGSLLVGL"
     gene            complement(408..980)
                     /locus_tag="PKW73_15585"
     CDS             complement(408..980)
                     /locus_tag="PKW73_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine-cytidine kinase"
                     /protein_id="HNA74770.1"
                     /translation="MAAGKRNSQIIVICGGSGSGKSTLAKKFIGASILSTDHFYKDLT
                     DLIPKEDGTFDFDHPSAVDLEECARACTNLLKGTAVNIPVYDMRSCKRVGEQIVPPPE
                     RSIVVVEGIFAFHSPLRELATLKIFIDTPIDQRMARRLRRDVERGRSTLDTIRHSLAV
                     EEAYTRYVEPMRELADLVLMGEEMNGSFSI"
     gene            1088..>1741
                     /locus_tag="PKW73_15590"
     CDS             1088..>1741
                     /locus_tag="PKW73_15590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNA74771.1"
                     /translation="MNPGENIEAGTVDQEHGQAGEDGNGQSGPDFTGFGFQDLINSVE
                     AIIWEADFKSSAITYVSNYVVELLGYSLDKWSQDPDLFASIVHPDDLQLIARAKGSVT
                     ALNNRYQVTYRVVKSSGEIVWLSDRVQVVFEASEPKLLRGISTDVSERRRVEEALALV
                     VEVTLAASELESVPDIAQVSLSRICELLDLCFGQVWFLDKGASFLKASPQVHFATLNR
                     "
BASE COUNT          392 a          434 c          512 g          403 t
ORIGIN      
        1 gcagccccac aaggaggcta ccgaatctag ccgtcaccga aaggtcgcag ccggagagcc
       61 ccggcagcgg cattaaaggc aagaggcggg cggtgaagac atggggcaga tagccgattt
      121 gcggcaggga gccgtagacg gccgaggggc ccccggctgc tacttctgcg gcttccggta
      181 agcgcaagta ctcggccatg aacttgagca cccagaagtg cgaaaattca tcgggagcac
      241 ccaccatcgg caccatgaag agccagggca agcgcagggc aaaataaaga agaatcagga
      301 tgcccagcgt cagcttcaga tgtctatccg gagcggatag cgactgttct aattttgggt
      361 cttccgtgga catctaaatg gctgcagcat tggggatagt tttagagcta aatggagaaa
      421 cttccgttca tttcctcgcc catcaaaacc aggtcggcca gttctcgcat gggctccacg
      481 tagcgggtat aagcttcttc cacggccagg ctgtgcctga ttgtgtcgag ggtggaacgc
      541 cctctttcaa cgtcgcggcg aaggcgcctg gccatgcgtt gatcgatggg cgtatcgatg
      601 aaaattttca gggtagccag ttctcttaaa ggagaatgga aggcgaaaat tccttcgaca
      661 actacgatgg agcgctccgg cggaggaacg atttgttcgc ccacgcgttt gcaactgcgc
      721 atatcgtaga cggggatgtt tacggccgta cccttgagaa ggttggtgca agccctggca
      781 cattcttcca ggtctaccgc cgagggatga tcgaagtcaa aagtgccgtc ttccttgggg
      841 atgaggtcgg tcaggtcttt gtagaaatgg tcggtggaga gaattgaggc accgatgaac
      901 ttcttggcta aggtggactt gccggagccg gatcccccgc aaatgacgat tatctggcta
      961 tttcgtttac cggcagccat ggtgacgcgc cttttctgga acctctgaga taatatctta
     1021 ggaagcaggt tcagaagttg cattaacaga ccattaaccc ttctagtaat aagcttgagg
     1081 cctcctggtg aatcctggcg aaaacatcga agctggaacg gttgaccagg aacacggcca
     1141 agccggtgaa gatggaaatg gtcagtcagg accagatttc accggttttg gcttccagga
     1201 tctgattaat tcggttgaag ccattatctg ggaagccgat tttaagtcct cggctatcac
     1261 ctatgtttcc aattatgtgg tggaactgct gggatactcg cttgacaagt ggtcgcaaga
     1321 tcccgacctc tttgccagta tcgttcatcc tgacgatttg caacttatag ccagggccaa
     1381 gggctcggtc acggccctca ataatcgcta tcaggttact tatcgggttg taaagtccag
     1441 cggggaaata gtctggctct cggaccgggt gcaggtggtt ttcgaagcct ccgagcccaa
     1501 gctcttgcgc ggcatcagta ccgatgtgag cgagcgtcgg cgggtggaag aagccctggc
     1561 tctggtggtg gaagtgaccc tggctgcctc ggaattggag agtgtgccgg atattgccca
     1621 ggtaagcctt tcccgtattt gcgagttgct tgatctttgc ttcggtcagg tctggtttct
     1681 tgacaagggg gccagttttc tcaaggcctc tccgcaagtc cattttgcca ccctcaaccg
     1741 g
//
LOCUS       DAOQNZ010000988         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50569_length_1739_cov_2.429335, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000988 DAOQNZ010000000
VERSION     DAOQNZ010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50569_length_1739_cov_2.429335"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PKW73_15595"
     CDS             <1..791
                     /locus_tag="PKW73_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNA74772.1"
                     /translation="KCGKLTRPGGLCFDMKGRLYVCDASDQYIRIFDGKGYFLSEFRN
                     IQAADSGIAGITFDTSGLLYISDSANSCIQVFQPETGVWLRSISTKSDLGQRRSGSSY
                     GSPYAASYSTAGHDGGRSAEAMELRLPAGLCVDRLNQIYLADYGASRIFVFNKQGQVV
                     KQFGQKGSTDGLLNIPRSVAVDKFDRIYVCDSFNNRLAVFDTGGGFLYNYGAPGPDTP
                     REEVLTTPSDIAIDRDFGLVFVADRGGKRVQILKLAQESSYEYR"
     gene            778..1428
                     /locus_tag="PKW73_15600"
     CDS             778..1428
                     /locus_tag="PKW73_15600"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNA74773.1"
                     /translation="MNTADQSAKSKQDRRIILASASPRRIQLLNNLALNFEVEPADID
                     ETVEAGVSPQRAVQELSAHKAAAILEKLRRQGEPGSNLDLIIIAADTLVADGEEVLGK
                     PQDREQAIDMLKQLAGKPHQVFTGVHLINLKNGKEEHYRECGATTVYFRNLQEEEITS
                     YVDTGEPMDKAGAYALQGIGAFLVEKIDGCAANVIGLPVPLVVALLRRIGLKVMGL"
     gene            1453..>1739
                     /locus_tag="PKW73_15605"
     CDS             1453..>1739
                     /locus_tag="PKW73_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74774.1"
                     /translation="MSETDGTRLENLPRPRNQEDPDFPIVSRLTAALQASGALKGKTI
                     AWHCHLTLLTELAARAVVAAGARLVLSECNPATTQVEAVENLEKIGRVFLG"
BASE COUNT          405 a          511 c          472 g          351 t
ORIGIN      
        1 ccaagtgcgg caagctcacc agaccgggcg gtctttgttt tgacatgaag ggtcgcctct
       61 acgtctgcga cgccagcgac cagtacattc gcatcttcga cggcaagggc tattttctca
      121 gcgagtttcg caatatccag gcggccgact ccggcattgc cggtatcact tttgatacca
      181 gcggtctgct ctatatctcc gactccgcca attcctgcat ccaggtattt cagcctgaaa
      241 ccggagtctg gctgcgctcc atcagcacca agtccgacct ggggcagaga cgttccggct
      301 cttcttatgg ctccccctat gccgcctcct attctacggc cggtcatgac ggcggacgca
      361 gtgccgaagc catggaattg cggctaccgg cagggttatg cgtggacaga ctcaatcaaa
      421 tttatctggc ggactatggc gcctctcgca tctttgtctt caataaacaa gggcaggtgg
      481 tcaagcaatt cggtcaaaaa ggtagcacgg acggacttct gaacattccc agatcagtag
      541 ccgtggacaa attcgaccgc atctatgtct gcgactcctt caacaatcgg ctcgccgtct
      601 tcgataccgg cggcggcttc ctttacaatt atggtgctcc cgggccggat acgcctcgtg
      661 aggaggtgct cacgactcct tccgatatag ccatcgatcg agactttggg ctcgtcttcg
      721 ttgccgaccg cggcggcaaa cgcgtgcaaa tattgaagct ggcccaggag agcagttatg
      781 aataccgctg accaatcggc gaaatcgaaa caggacaggc gcatcatcct ggcctcggct
      841 tcgccaagac gcattcaact gctcaataat cttgccctca acttcgaagt agaaccggcc
      901 gacatagacg aaacggtaga ggccggcgtc tctccacaac gggccgtaca agagctttca
      961 gcacacaagg ccgctgccat attggagaaa ctgcggcggc aaggcgagcc cggctcaaac
     1021 cttgatctca taatcattgc cgccgacact ctcgtggcag acggagaaga ggttctgggc
     1081 aagccgcagg accgcgagca ggccatcgac atgcttaaac agttagcagg caagccccat
     1141 caagttttca ccggcgtgca tctaatcaat ctcaagaacg gcaaagaaga acactaccgg
     1201 gaatgtggtg ccacgaccgt ctacttccgc aatctgcaag aagaagaaat cacaagctat
     1261 gtggacaccg gtgagccgat ggacaaagcc ggcgcttatg cactgcaggg tatcggcgcc
     1321 ttcctggtgg aaaaaatcga cggctgcgcc gccaacgtga tcggtctacc ggtaccgctg
     1381 gtagtggcac tactgcgccg tatcggtctc aaggtcatgg gtctctagag aagaaaggaa
     1441 gcaggaggtc ttgtgagcga gactgatgga accaggcttg agaacctgcc ccgccctcga
     1501 aaccaggaag atccggattt tccgattgtc agccgtttga ccgccgccct ccaggcaagc
     1561 ggtgccctca agggcaaaac cattgcctgg cactgccacc tgacactcct gacggaactg
     1621 gcagccagag cggttgtagc ggccggcgcc cgcttggttt tgagcgaatg caaccctgcc
     1681 accacccaag tggaagcagt tgagaatctt gagaaaatcg gccgggtatt cctgggcca
//
LOCUS       DAOQNZ010000989         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50595_length_1738_cov_3.773024, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000989 DAOQNZ010000000
VERSION     DAOQNZ010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50595_length_1738_cov_3.773024"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="PKW73_15610"
     CDS             complement(<1..1675)
                     /locus_tag="PKW73_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74775.1"
                     /translation="MKFPRKSKRANRGSIIVLATVLALTIVVLGGGFLFFELFMGAQK
                     ETKNAVDAGVLNVAKQVLDKSNAQVSVILPGGPTSCFWDVTCDEPASYNMGDGIINLK
                     RVNRMWGKAMLIAINAAAAQSDGNAGSGPGNAQQAFNQAQSVSDQLTDKLNNQTNLHG
                     FFANLASQNSVRMLGLGAEVKPLPGNGWETSLMERGAESNISMNGSPPSFNLPPGYSL
                     NNSFVTKSTRNPVPASGANLWFLKGYIPLAIGNQTFWQVPFKYDEKPHHVSRSLFDAS
                     TMKNNPLSWNKAVPNAFSGEGEAIKKSPDGQKATSWVLTNPRENFQLSMPHSFVKIHL
                     DDMESHWYFPLSPTPMLKWSTQSYGYTPSSQSSLMPAGGPLSVSVSGTFLIGLDVVGR
                     TLDGVIFDPPFSGNSSKVEAYLTNRCNEMMTVPGTTKTTAQMHAALSDPSTILAMVGG
                     VRDFYLFSPDGMSLSCKPQPLAIAQSPWLATMIAQSADGTETKLIDGATFPAGLGIPP
                     MSTVVPDPFCTPVPLPVVGFWYKDVAWTPGSGYNGNLGLIRVKRWTEVQV"
BASE COUNT          345 a          433 c          542 g          418 t
ORIGIN      
        1 gaacctgcac ttccgtccag cgcttgacgc ggatcagacc caggttgccg ttgtagccgc
       61 tgcccggtgt ccaggctacg tccttatacc agaagcccac taccggtagt ggcactggcg
      121 tacagaaggg atctggcacc actgtggaca tggggggaat acccagcccg gcagggaagg
      181 tggcgccgtc gatcagtttg gtctcggtgc cgtcggcgct ctgcgcgatc atggtggcca
      241 gccagggcga ctgggcaatg gctagaggct gcggcttgca agataagctc atgccgtccg
      301 ggctgaagag ataaaagtct ctgacgccgc ccaccatggc tagaatggtg gagggatcgg
      361 agagcgcggc gtgcatttgc gctgtggtct tggtcgtgcc cggtaccgtc atcatttcgt
      421 tgcaacggtt ggtcaggtaa gcttccactt tgcttgagtt gccgctgaag ggcgggtcga
      481 agataacacc gtccagggtt cttccgacca cgtccagtcc gatcaggaag gtacctgaga
      541 ccgatacgct cagaggaccg ccggcgggca tgaggctact ctgggaactg ggagtgtagc
      601 cgtaactctg ggtggaccat ttcaacatgg gcgtggggct taagggaaag taccagtgtg
      661 attccatgtc gtccaggtga attttcacga aagagtgagg catggagagc tggaagtttt
      721 ctctcggatt ggtcagtacc caggatgtgg ctttctgtcc atcgggagat ttcttgatag
      781 cttccccttc gccggagaag gcattgggaa cggccttgtt ccaactgaga ggattgttct
      841 tcatggtgga agcatcgaaa agtgatcttg agacatggtg tggcttttca tcgtatttga
      901 aaggcacctg ccagaaggtc tggttgccta tggcaagcgg aatataacct ttcaaaaacc
      961 agagattggc gccgctagct ggtaccggat ttctggtgga cttggtgaca aagctgttgt
     1021 tcaagctgta accggggggc aggttgaagc ttggcgggct gccgttcata gaaatattgc
     1081 tttcggcacc gcgctccatg agggaagttt cccagccgtt tccgggcagg ggtttcactt
     1141 cggctcccag accgagcata cgcaccgagt tttgcgaggc cagattggcg aaaaatccgt
     1201 gcaagttagt ttgattgttg agtttgtcgg tgagttgatc gctgactgat tgggcctggt
     1261 tgaaggcttg ctgggcattg ccgggccccg agccggcatt gccgtctgat tgcgcggcgg
     1321 cggcattgat ggcaataagc atggctttgc cccacatgcg gttgacgcgc ttcaaattga
     1381 taatgccgtc gcccatgttg tagctggccg gttcgtcgca ggtgacatcc cagaagcaag
     1441 aggtgggacc acccggcagt atcaccgaca cctgggcatt gcttttgtcc agaacttgct
     1501 tggcgacgtt cagtacaccg gcatcgacgg cgttttttgt ttctttctgg gcgcccatga
     1561 agagttcgaa gaagagaaaa ccgcccccta aaaccacaat cgtgagggcc agtaccgtgg
     1621 ccaggactat gattgagcct cgattggcgc gcttgctttt gcggggaaat ttcatggcga
     1681 tttctctcat ctgacttgct tactttactt ggtgccgact accggttcgg ctgcctgg
//
LOCUS       DAOQNZ010000990         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50596_length_1738_cov_3.675579, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000990 DAOQNZ010000000
VERSION     DAOQNZ010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50596_length_1738_cov_3.675579"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PKW73_15615"
     CDS             <1..1213
                     /locus_tag="PKW73_15615"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA74776.1"
                     /translation="SLLLREAKEEKIWTDPLTKLRNRSYLQEVVTDLTTTINVTSGQT
                     YAIAYFSVNSETGSTDDNLDNYALCVAKALSHLKETQHLSKLGDLSKLEFIRAQPLEF
                     ALIIRGENETFYEECCLELSRLVETSLRAERTTGHHLVIGLGLALFPFDALKGEDVLA
                     RAQLAMVLAEQLAKPEQNRAVPGGYRVTTSLAKAKHIPANFKPLRKFSAVKGELGVLD
                     AAEVIQSMASGGRTGILSVEDGVLKRTFTLLIQDGKPIGASLDGLLGMDALVEFVSTF
                     ETGSFIFSEKDSLENEAPMVRRQAMPSLVNGLMDAALASDNLAAARQIIKDLKTPIIA
                     QYSDAAWRELFRQEEPGEREQHLLKEVMRMANGRHSLEAIFAQLKYAPTAMVWHATAR
                     LASHGLINYAR"
     gene            1407..>1738
                     /locus_tag="PKW73_15620"
     CDS             1407..>1738
                     /locus_tag="PKW73_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74777.1"
                     /translation="MSYRNNTARSLTSGRRTRGMTMLAAELSIFVPLIVVGGFLLLDA
                     GLMSLQKQKLSFVLIQTANYLVNLPPEDDPIKPAEGLIKDLCKKSGLSYSNLNIKVEK
                     TSIGDNEAL"
BASE COUNT          470 a          506 c          418 g          344 t
ORIGIN      
        1 cagcctgctt ctcagagaag ccaaagaaga aaagatctgg accgacccgc tcactaaact
       61 aagaaaccgt tcttatttgc aggaagtggt taccgatcta accaccacca tcaatgtcac
      121 cagtggtcag acctatgcca tcgcctactt cagcgtcaac tccgagaccg gcagcaccga
      181 cgacaatctc gataactacg ctctctgcgt agccaaagcc ctcagtcatc taaaggaaac
      241 ccagcatcta tctaaactgg gcgacctctc caagttggaa ttcatcagag cccaacccct
      301 ggagtttgcc ctgatcataa gaggcgaaaa cgaaaccttc tacgaagaat gttgcctgga
      361 gctgagtcga ctggtagaaa cttctttgag agcagagcgc accaccggac accatctggt
      421 tattggactg ggactggcgc ttttcccctt cgacgccctc aaaggcgaag acgttctggc
      481 cagagcgcag ctagccatgg tactggctga gcaactggcc aaaccggaac agaaccgcgc
      541 ggtaccaggc ggctatcggg tcacaacatc tcttgccaaa gccaaacata ttcccgccaa
      601 cttcaagccc ctgcgtaaat tcagcgccgt caaaggagaa ctgggcgttc tcgatgcggc
      661 cgaagtaatt cagtcaatgg ccagcggcgg acgcacaggc attctcagcg tggaagacgg
      721 ggttctcaaa cgcaccttca ccctcttgat tcaggacggc aagccgattg gcgccagcct
      781 ggacggacta ctgggcatgg acgccctggt ggaattcgta tctacctttg aaacaggctc
      841 tttcattttt tcggaaaagg acagcctcga aaacgaagcc cccatggtca ggcggcaagc
      901 catgccctct ctcgtaaacg gactgatgga cgccgccctg gcctccgaca atttagccgc
      961 cgccaggcag atcatcaaag acctcaagac cccaatcatc gctcaatact cagatgccgc
     1021 ctggcgagag cttttccgcc aggaagagcc cggcgaaaga gaacagcatt tgctcaagga
     1081 agtgatgcga atggctaacg gccgccacag cctggaagca atttttgccc agttgaaata
     1141 cgcccccaca gccatggtct ggcacgccac agcccgcctg gccagccatg ggttgatcaa
     1201 ctacgcaagg taagtagtca aaaaaagata ctcactttcg cccatggctt tcaaaatggg
     1261 gcggctgcat aatgagcctc cggtcgaaag actaagtact agcacaatcg cagcagaacc
     1321 cggtctctca agagataccg gtgcttgatt ctggagagga gcgcccctcc tctttagcaa
     1381 tgcgtccact taagaggtaa agtcgaatga gttatagaaa caacaccgcc cgttcactta
     1441 cctcgggtcg ccgcaccaga ggcatgacca tgctggcagc cgaattgagt attttcgtgc
     1501 cgctcatagt agtcggcgga tttctcctgc tcgatgccgg actgatgagc ctgcaaaaac
     1561 agaagctctc cttcgtattg attcaaactg ctaactacct ggtcaatcta ccgcccgaag
     1621 acgatcctat caaacccgcc gagggactga ttaaagacct gtgtaaaaag tcggggctca
     1681 gttattccaa tctaaatata aaagtagaaa aaacaagcat cggcgacaac gaagccct
//
LOCUS       DAOQNZ010000991         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50648_length_1737_cov_4.570749, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000991 DAOQNZ010000000
VERSION     DAOQNZ010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50648_length_1737_cov_4.570749"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKW73_15625"
     CDS             <1..229
                     /locus_tag="PKW73_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74778.1"
                     /translation="LAFTKEISVILEHCYASRRVKVVRVPAMPPTERIQFVMGKIEEI
                     KKEQVFSVEQAVHKASLYFNPAMPGLVTTAY"
     gene            297..1562
                     /locus_tag="PKW73_15630"
     CDS             297..1562
                     /locus_tag="PKW73_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNA74779.1"
                     /translation="MRASEATNYYFKKAAKALRLGAGLETVLMNPRREVKVECVIELD
                     NGELGTFTGFRVQHDNSRGPFKGGLRYHPEVDPDEVNSLASLMTWKTAVANIPYGGAK
                     GGINCDPSKLSHSELQKVTRAFIDEIHDIIGPGLDIPAPDMGTNSQTMAWIVDQYSKY
                     HGWTPAVVTGKPLELGGSEGREQATGQGLFFVIESVLWDLGMDLGKTRFVVQGFGNVG
                     SWVARFIHGGGGKVVAIADVSGAVYNKDGLDVDALWHHSLENGSVNGFAGGEATDPKK
                     LFTLDCDVLVPAALGNVITKENMQDIKAKVVVEGANAPTTPEADEYLHKKGITVVPDI
                     FANSGGVIVSYFEWVQNVQQFRWKLDRVNQELKSQLLDSYKEIKHIERSSECDMRTAA
                     FQLAISRVARTTALRGLENENFCMLSAPH"
BASE COUNT          446 a          465 c          480 g          346 t
ORIGIN      
        1 cctggccttc accaaagaaa taagcgtcat tctggaacat tgctatgcaa gccgcagagt
       61 taaggtggtg cgagtaccgg ctatgcctcc caccgagcgc attcagtttg ttatgggcaa
      121 aatcgaagaa atcaaaaagg agcaggtgtt ttcagtagaa caggccgtac ataaagcctc
      181 cctctacttc aatcctgcca tgcccggctt ggtcaccacc gcctactgag gaaagcttat
      241 cctctttaga gcgccttaga gatgctaacc tattagcatt tcctaaggag gcgataatgc
      301 gagcatctga agccaccaat tattacttca aaaaagcagc caaggccctg agactgggag
      361 ccggtctgga gaccgtgctc atgaatccca ggcgggaagt gaaagtagaa tgcgtcatcg
      421 aactggacaa cggtgagctt ggcactttta ccggcttcag agtgcagcac gacaacagtc
      481 ggggaccttt caagggcggc ttgcgttatc acccggaagt agatcccgat gaggtaaaca
      541 gcctggcctc gctcatgacc tggaagacgg cagtggccaa tattccctac ggtggagcga
      601 aaggcggcat caactgcgac ccttccaaac tctcccattc ggaactgcag aaagttaccc
      661 gcgccttcat cgacgaaatc catgacatca tcggtcctgg tctggatatt cccgcccccg
      721 acatgggcac caattcccag actatggcct ggatagtaga ccagtactcc aagtaccacg
      781 gctggacacc ggccgtagtc accggtaaac ccttagaatt gggcggcagc gaaggaagag
      841 agcaggccac cggacaggga ctgttctttg tcatcgaatc ggtgctctgg gatctgggca
      901 tggaccttgg caagacccgt tttgtggtgc agggcttcgg taatgtgggc tcctgggtgg
      961 cgcgcttcat tcacggaggc ggcggcaaag tggtagccat agccgacgtc agtggcgcgg
     1021 tgtacaacaa agatggtctg gatgtagacg cgctctggca ccacagcctg gagaacggct
     1081 cagtaaacgg ctttgccggc ggcgaagcca ccgaccccaa gaaactcttc acccttgact
     1141 gcgatgtctt ggtaccggca gccctcggca atgtgattac aaaagaaaac atgcaggaca
     1201 tcaaggctaa agtagtggtg gaaggtgcca acgcccctac tacaccggaa gccgacgagt
     1261 atctgcataa gaagggcatc acagtggtgc cggatatttt cgccaattcc ggcggcgtca
     1321 tcgtctctta ttttgaatgg gtgcaaaacg tccagcagtt cagatggaaa ctagacagag
     1381 tcaaccagga attgaaaagc cagttgcttg attcctataa agagataaag catattgaaa
     1441 gaagttcgga gtgcgacatg cgcactgccg ctttccaact ggccatcagc cgcgtcgcca
     1501 gaacaaccgc cctgcgcgga ctggagaacg aaaacttctg catgctttca gcgccccact
     1561 aaagctcaat gagtgaagaa gagaaaagca tggagccggg ctccctgccg gagcaacatc
     1621 aagacttgct ggagcgggat ctcgacgaca aacaatggtt tgagacggtt taccagggcg
     1681 accgggtgcc gcagctaacc atgcgcgccg tgcttatggg aggcgtgctc ggcgccc
//
LOCUS       DAOQNZ010000992         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50676_length_1737_cov_3.256243, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000992 DAOQNZ010000000
VERSION     DAOQNZ010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50676_length_1737_cov_3.256243"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PKW73_15635"
     CDS             complement(<1..473)
                     /locus_tag="PKW73_15635"
                     /inference="COORDINATES: protein motif:HMM:NF021584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HNA74780.1"
                     /translation="MTLQLLSLLCLPTLLTACSQSGDNTAAAGQTAQTANASGETSGT
                     EGGEPKWNISRRASDDPEIAAAKSEAQKHWHMFRDKFASAQSKSQFSIKVPFSEGGQT
                     EHMWLEVNSIDGEEISGTLSNTPDVLKNMREGQIIKVPRSEIEDWSYPAEGKQIGG"
     gene            775..>1737
                     /locus_tag="PKW73_15640"
     CDS             775..>1737
                     /locus_tag="PKW73_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74781.1"
                     /translation="MESHKPIKFSHAGSGLSLGSGAGSLDVGADAGDSSVGTGQKHAR
                     GRLQMASLDGRGDILRQTSTTDNTAATNLQPMTLTEGALSPKKSLDDLVKAGVIADST
                     AKGRDNFSDTTKSEPRPITSFEKPKIEVGFADTLEQQGKKPDLIVKADGRVEMINNPE
                     ILSSGNVVVGLERPDGAVNPTDAQQKSVAELYKYLDARLKEQNPALAGKGIDLENPQG
                     LVPEEAAAAARRAGQRANVPDGSDAGAVQGGNRFTGSRSGGRLSSGEVSDMFPGRDFK
                     ARNGKVDAVELIKDTVSSLNGSKGDHEHIGYRRGRGHAVGAYGMT"
BASE COUNT          380 a          441 c          493 g          423 t
ORIGIN      
        1 ccgccgattt gcttgccttc agcaggataa gaccagtctt cgatttccga tctcggcact
       61 tttataattt gaccttctct catatttttc agaacgtcgg gcgtattgct cagggtgccg
      121 ctgatttctt cgccgtctat ggaatttact tccagccaca tgtgctcagt ttgcccccct
      181 tcgctgaagg gaaccttgat gctgaactgt gattttgact gggcgcttgc gaatttgtcg
      241 cgaaacatat gccagtgctt ttgcgcttca ctcttggcgg cggcgatttc cggatcatca
      301 gaggctcgtc tgctgatgtt ccactttggc tcgccgccct cggtgccgga agtttcacct
      361 gaagcgtttg ctgtctgtgc ggtttggccg gctgcggcgg tgttgtctcc gctctgggag
      421 caggccgtga gtagtgtggg caggcagagc agggagagga gttgcagggt cattgacctt
      481 ttcattcgta ggcggccttt ttagcttgcg ggataaagcg attctagcag tggaagctgg
      541 gcctgttaaa gagcgcccga ctcggcatag gggggcgctt tcgctagttt tgcagtcaat
      601 attggcaatt tgggggctaa attaatgcaa ttcatgggat tcctacgtag ccggcccact
      661 gtgcactttc ggctatctga catatattga ggcatggttt tcgaaacagc cttgatgtgc
      721 tgaaccttag ctccccaaaa acccccgatt tcttaaggta cggagaaaac tactgtggaa
      781 tcacataaac ctattaaatt ttcccacgcg ggttccggcc tgtcgcttgg ttccggtgcc
      841 ggctccttag atgtgggagc agatgccggg gatagttccg tgggaactgg gcagaagcac
      901 gccagaggac gcctgcaaat ggcctcgctg gatggtcgcg gtgatatttt gcgccagacc
      961 agcacaacag acaacacggc ggcaaccaat ttgcagccca tgaccttgac agaaggggcg
     1021 ctcagtccta agaagtccct cgatgatctg gtaaaagccg gtgtaattgc cgattctaca
     1081 gcaaagggtc gcgacaactt cagcgacaca actaagtctg agcccagacc tattacttcc
     1141 tttgaaaagc cgaagatcga agtaggcttt gccgacaccc ttgaacagca gggcaaaaag
     1201 cccgacctga ttgtaaaagc cgatggtcgg gtggagatga tcaataatcc cgagatcttg
     1261 agcagcggta atgtagttgt aggtcttgag cgtcccgatg gcgccgtcaa tcctaccgat
     1321 gctcagcaga agtcggtagc tgaactatac aaatatctgg atgccagatt gaaggaacag
     1381 aatccggctc tggcaggcaa aggcatcgat ctagagaatc cccagggcct ggtgcccgag
     1441 gaagcagccg ctgctgctag aagagctgga cagcgagcca atgtacccga tggttccgat
     1501 gccggtgccg tgcagggcgg caatcgtttc accggttcca gaagcggtgg acgtctttcc
     1561 tccggcgaag tgtccgacat gttccccgga cgtgatttca aagcccgcaa cggtaaagtc
     1621 gacgctgttg aactgatcaa agatactgtt tccagcttga atggttccaa gggcgaccac
     1681 gagcatatcg gttatcgccg tggacgtggt catgccgtgg gcgcctacgg tatgacg
//
LOCUS       DAOQNZ010000993         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50677_length_1737_cov_3.256243, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000993 DAOQNZ010000000
VERSION     DAOQNZ010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50677_length_1737_cov_3.256243"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1380)
                     /locus_tag="PKW73_15645"
     CDS             complement(121..1380)
                     /locus_tag="PKW73_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74782.1"
                     /translation="MFEKVLSKFSVFVKYCLAPLSDQSTSRLDSWPVKLCALGLIVGM
                     TFSAPLWLNFGRTLPVCALFPLGQLALLTPLLSIVTVFSAFSLVLVGVNRLAAGLLLL
                     SMSALVLMDLNRLQPWVYEYCLIILLFTIADSSPEYKEKRFQYLGLIIMAIYLFSGLE
                     KLNWRFLTGIGPWLGGFSPSPALCLSGDQNAVAVSLLMCLGEIFMALLLVFRRTRFLG
                     ICGCMFMHLTILAMLGPQGLNINAVVWPWNIFQMLLMAGCFMELQNSGLAFLRLDFSR
                     NRKASSLVVFAGVLLPVLGLFQLADPYPSFSFYSGDVPRGRLIFKAATLQKLPPQLQR
                     ICRYDRVNALCFLDFADWALIETTAPAYPSKFCLLSMAKAFVATYQPDCLVLRISTFP
                     KFTCREMREEVLFKMNSQVVLISGQEQ"
BASE COUNT          556 a          429 c          437 g          315 t
ORIGIN      
        1 cctgcctgtt gtctatggca cagtcggcgg cagaactgca gccgcccagt gccagcgcca
       61 gtggaagaaa aaatacggcc aaaaatggct tgtactgcct ggttttgatg cgacttatac
      121 tcattgctct tgccccgata ttaagaccac ctgagaattc attttgaata aaacttcctc
      181 cctcatttcc cggcaggtaa atttggggaa ggtagagatt ctcagaacca aacagtcagg
      241 ctggtaggta gctacaaaag ccttggccat ggaaagcaga caaaacttac ttggataagc
      301 cggggcggtg gtttcaatca acgcccaatc agcgaaatcc aggaagcaga gcgcattcac
      361 tcggtcgtaa cggcagatgc gctgcaactg cggcggcaat ttttgcagcg tagcagcctt
      421 gaagatcagt ctaccgcggg gcacatcacc agagtagaag gaaaacgaag gatagggatc
      481 cgccagttga aagagcccca acacaggcag taacacacca gcaaacacga ccagagaaga
      541 ggccttccgg tttctggaaa agtcaagccg caagaaggca agaccggagt tttgcaactc
      601 cataaaacaa ccagccatga gcaacatctg aaaaatattc cagggccaga ctacggcatt
      661 gatattcaac ccctggggtc cgagcattgc aagaatggta aggtgcataa acatgcaacc
      721 acaaatgccg agaaagcgtg tgcgcctgaa aaccaaaagc aaagccatga agatttcacc
      781 aaggcacatt agtaaagaga cagcgacggc attttgatca ccggagagac agagagccgg
      841 agaaggcgaa aagccaccca accaggggcc tataccagta aggaaacgcc agttgagctt
      901 ttccagtcca ctaaacaaat atatagccat gatgatcaga cctagatact gaaatcgctt
      961 ttccttgtac tccggagagc tgtcggcaat ggtaaataga agaatgatca gacaatactc
     1021 atagacccag ggctgcaacc ggttcaagtc catgagaacc agggccgaca tgcttagaag
     1081 tagaagtccg gcagcaagcc tattgactcc caccaggacc agagagaaag cactaaatac
     1141 ggtgacaata ctaagtagag gcgttaggag cgccaactgc ccgagaggga aaagcgcgca
     1201 caccggcagg gttcgaccaa aattcaacca gaggggggca gagaaggtca tgccaacaat
     1261 caagccgagg gcgcaaagct tgaccggcca gctatccaaa cgagacgtag actgatcact
     1321 caggggtgcc aggcaatatt ttacaaacac gctaaacttc gaaagaactt tctcaaacat
     1381 ttttgacact ccaaagaaag agcaacgcct acaaggcgcg cttaaaatga gaggcacggc
     1441 aaaacaccac cggcataaca ccggcttggg cagaggagat ggcagaagct attcgaaccg
     1501 actaaatggc ttttgaaaga gcaggcaata aaggtccgct acatgagcag attatggggc
     1561 cgagagcctc ccaggtatat gggcgaaagc gagtattttg cctgagcgca aattgagaaa
     1621 cctggagatc agtctttcaa taggaaacca gcgccacgcc attctgaaaa ggcccggcct
     1681 ctttaaactg gtagtcgaca acaagcttgc cggtcttatc aatgtagcca aattttc
//
LOCUS       DAOQNZ010000994         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50772_length_1735_cov_4.510714, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000994 DAOQNZ010000000
VERSION     DAOQNZ010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50772_length_1735_cov_4.510714"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKW73_15650"
     CDS             complement(<1..246)
                     /locus_tag="PKW73_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74783.1"
                     /translation="MSKDTARDKTPAKPELHDATEDDTRHKPADSFRGEYLSVKEKER
                     VEKPKAEKPADADKERDGSLSINGVPRNLDLSEEESGA"
     gene            456..1142
                     /gene="lexA"
                     /locus_tag="PKW73_15655"
     CDS             456..1142
                     /gene="lexA"
                     /locus_tag="PKW73_15655"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNA74784.1"
                     /translation="MHSSKPLPARQQEVLSLIVSFSEEHGYPPTLADLAQCLGLKNRM
                     TVHQHVAALRKKGLVHWEPGLNRSLRVLPEGLQHLDPGVEVGAEAVQPAAASNVVGFP
                     AAAGIPLAGAIAAGSPIDALQTSEYLEVESRYGEIGCFALKVKGESMIEDGIYDGDFV
                     IVKPNPSPNNGDVVVALLDDGSATLKRFYKEKGGYRLQPANASMEPIFVDGSHPLTIQ
                     GTVVGLFRQM"
     gene            complement(1157..>1735)
                     /locus_tag="PKW73_15660"
     CDS             complement(1157..>1735)
                     /locus_tag="PKW73_15660"
                     /inference="COORDINATES: protein motif:HMM:NF017482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HNA74785.1"
                     /translation="ASRSIEEAQQRRQALTVRLQQVLEARDIYDLKQIKEGLSGKKPQ
                     DIVKPRGNGAKGKGKNEKIISVNSSDGWTIYVGRNRMENDYLLSRLAQPNDLWFHVLG
                     QGGAHVLIRIPSSKQDPPARTLEEAGQIAARFSKAGHGAKVRVIYTQCKYVRKLAGDK
                     PGLVRYENEKSLEIDTGKPMPKAMKQLFAPGQ"
BASE COUNT          320 a          494 c          477 g          444 t
ORIGIN      
        1 agctccggat tcttcttccg acagatcgag gttgcgcggc acaccgttta tggaaaggct
       61 gccgtccctt tctttgtcag cgtcggcggg tttctcagcc ttcggtttct cgacccgctc
      121 cttctctttc accgagagat attcgcccct gaaggagtcg gcgggtttgt gcctggtatc
      181 gtcttcagtg gcgtcatgaa gttccggctt agccggggtt ttgtccctcg ccgtatcttt
      241 gctcatgaaa actctctgct ccggcttcct ttactatcag tctcttctat atttctgccc
      301 ccggccgctc caccttaatc ttttgcccgt cccagagaaa ggctcgacaa aatgtagtaa
      361 gtggtactat acttacgtat gtggcgtacg tatgtagtct aaagtatgtg gtctagatgt
      421 gtagcctaaa tcaatcccag ccggaggact aacagttgca ttccagtaag ccgcttcccg
      481 ccaggcagca ggaagtcctc agtctgatcg tcagcttttc cgaagagcat ggctatccgc
      541 ctactctggc agacctggcc cagtgcctgg gtctgaaaaa tcgtatgacc gtgcaccagc
      601 acgtggcggc cctgcgtaaa aaagggcttg tgcactggga gccgggtttg aaccgttcct
      661 tgcgcgtcct gcccgagggg ctccagcacc ttgatccagg cgtagaagtc ggcgctgaag
      721 cagtgcaacc ggcggctgcc tccaatgtgg ttggcttccc tgccgcagcc ggcataccgc
      781 tagccggggc tatcgccgcc ggcagtccta tcgatgccct gcagacaagc gaatatctgg
      841 aagtggagag ccggtacggt gaaatcggtt gcttcgccct caaggtcaag ggtgaatcca
      901 tgatcgaaga cggcatttat gacggtgatt tcgtcatagt caaacccaat ccctcaccca
      961 ataacggcga tgtggtagtg gctttgctcg atgacggcag cgccaccttg aagcgcttct
     1021 acaaagaaaa aggaggttac cgcttgcagc cggccaatgc ttccatggag ccaatctttg
     1081 tcgacggctc ccatcccctc acaattcaag gcactgtggt gggtcttttc cgccagatgt
     1141 agtctgactt gctttgctat tgccccggtg caaacagttg cttcatggct ttgggcatgg
     1201 gcttgcccgt gtcgatttcc agtgactttt cgttctcgta gcgcaccagc ccgggcttgt
     1261 cgccggccag tttgcgtacg tatttgcact gggtgtagat cactctaact ttggcgccat
     1321 gaccggcttt tgagaagcgg gccgctattt gtccggcttc ctccagggtg cgggcgggcg
     1381 gatcttgctt ggatgaggga atgcgaatca ggacatgggc gccaccctgg ccgagcacat
     1441 ggaaccagag atcattgggc tgggccaggc gcgaaagcag gtaatcgttt tccatgcggt
     1501 tgcgacccac ataaatggtc cagccgtcgc tggagttgac tgagattatc ttttcgttct
     1561 tgcctttgcc cttggcgccg ttgcccctgg gcttgactat atcctggggt ttcttgccgg
     1621 acagtccttc cttgatctgt ttcaaatcat agatgtcccg cgcctccagc acctgctgca
     1681 gcctgaccgt aagggcttgc ctacgctgct gcgcttcctc aatggaacga gaggc
//
LOCUS       DAOQNZ010000995         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50955_length_1732_cov_2.795468, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000995 DAOQNZ010000000
VERSION     DAOQNZ010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50955_length_1732_cov_2.795468"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PKW73_15665"
     CDS             <1..598
                     /locus_tag="PKW73_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNA74786.1"
                     /translation="HAGDVDIARQLAKENMAHFENLGGEIVVASAGCGAMLKGYEHLF
                     AEGEPERARAAQFSRRVKDFSQVMASHLPPQPQKRESGAKEAPRVAYHAACHLAHAQG
                     VRAEPEAVLNRLAAEGCLKRVPLADAEHCCGSAGIYNLLNTELSLQVLEAKMDSLQAS
                     GAEVVVTANPGCLLQLEAGVRARGLNVKVKHLAELCRD"
     gene            703..1359
                     /locus_tag="PKW73_15670"
     CDS             703..1359
                     /locus_tag="PKW73_15670"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNA74787.1"
                     /translation="MKSEVLVKHRKPDLFEDDIPDQRLEHYLTKKVVAIDTETRGLII
                     RRDRLCLIQICDEDGVVSFVRYSDKEKMSADAQSNIKTLCHAPTVTKLFHYGRFDLAV
                     LKYYLGIDVNPVFCTKIASKLVRTYTDRHSLKELVRELLGIELDKSDQTSDWAQPHLT
                     DSQLEYAANDVRVLLPIYERIHALLVREGRLDVHRRLCEALPTVCELDLMGFKDIFEH
                     "
     gene            1391..>1732
                     /locus_tag="PKW73_15675"
     CDS             1391..>1732
                     /locus_tag="PKW73_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74788.1"
                     /translation="MEKLIYEKSRAGRRAEILPKPQVPVKELSSFIPAKFLREKPPAL
                     PEVGELDAVRHFVRLSHLNHCIDTGFYPLGSCTMKYNPKVNDAMAALEGFRDLHPHQP
                     QDQIQGALELLY"
BASE COUNT          386 a          430 c          482 g          434 t
ORIGIN      
        1 ccatgccggc gatgttgata ttgcccgcca gttggcaaaa gagaacatgg ctcattttga
       61 gaatctcggc ggtgaaattg tggttgcatc cgccggttgc ggcgccatgc tgaaaggcta
      121 tgaacacctt tttgccgagg gagagcccga gcgagcccgg gctgctcagt tttcccggcg
      181 ggtgaaagat ttttctcagg ttatggccag ccatctgccc cctcagccgc agaagcgtga
      241 gagcggtgct aaagaggctc cccgggtggc atatcatgcc gcctgccacc tggcccatgc
      301 ccagggtgta agggccgagc cggaagccgt cttgaaccgg ctggccgcgg aaggttgttt
      361 gaagcgggtg cccctggctg atgccgagca ttgctgtggt tctgccggta tctataatct
      421 gcttaatacc gagctttcct tgcaggttct cgaagccaag atggacagcc tacaggcctc
      481 cggtgcggag gtggtggtta cggccaatcc cggctgtctc ctgcaactgg aggcgggagt
      541 gcgggccaga ggtcttaatg tcaaggtcaa gcatctggct gaactttgcc gggactgaga
      601 ttctcgtaag cgagaacatt attagaaccg ctctatcttt ggggctgagc cgttttatcc
      661 ctctataatt tgttttgttg aagcaagcaa attagaggtc tgatgaagtc tgaggtattg
      721 gtcaagcacc ggaagcccga tcttttcgag gatgatattc ccgatcagcg gctagagcac
      781 tatttgacca agaaagtggt ggcaatcgac accgagactc gcggactgat catccgccgc
      841 gacaggctct gtttgatcca gatatgcgat gaagacggcg tcgtcagttt tgtgcgctac
      901 agcgacaagg aaaagatgag tgcggatgct caaagcaata tcaagactct ttgccatgca
      961 cccaccgtca ccaagctttt tcactatggc cggttcgatc tggcggtact gaagtattac
     1021 ctgggcatcg acgtcaatcc ggttttctgt acaaagattg cttccaagct ggtgcgaacc
     1081 tataccgatc gccacagtct aaaagagttg gtcagggaat tgctcggcat cgaactcgat
     1141 aagtccgacc agacaagtga ttgggctcag cctcatttga ctgattctca gttggagtat
     1201 gcggccaacg acgtgcgtgt tttgcttcct atttacgagc gcattcatgc cctcctggtg
     1261 cgggaaggtc ggcttgacgt gcaccgtcgc ctttgtgagg cgctgccgac ggtttgtgaa
     1321 cttgatttga tgggatttaa agatattttc gaacactaaa ttttggtgcc ggtgttaaca
     1381 ggggaatgaa atggaaaagt taatttacga aaaatcaaga gcaggaagac gtgccgagat
     1441 tttgcctaaa cctcaggtgc cggtgaagga gttgtcttcc ttcattcccg ccaagtttct
     1501 gcgtgaaaaa ccgccggctc taccggaagt cggtgagttg gatgcggttc gccacttcgt
     1561 aaggctttct catttgaacc attgcatcga taccggtttt tatccccttg gttcttgcac
     1621 catgaaatac aatcccaagg ttaatgatgc catggccgcg ctggaaggct tccgcgactt
     1681 gcatcctcac caaccgcaag accagattca aggtgccctg gaacttctct at
//
LOCUS       DAOQNZ010000996         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_50965_length_1732_cov_2.450209, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000996 DAOQNZ010000000
VERSION     DAOQNZ010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50965_length_1732_cov_2.450209"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PKW73_15680"
     CDS             <1..650
                     /locus_tag="PKW73_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74789.1"
                     /translation="TMTSFVYGGMQLSTAKVDTFASITKLLDARAAVYSVNASLNRQL
                     LDKALRTEHRKNLTASLTELVGDLNDGQWQELEKNLSADKRPAADAKGSLVQAAATFD
                     FEDEFVVLQRVFQELRQFRQTIDQVHTLLDAGKAEEALKLSIGLNVGQGRYLFDKLDA
                     SLSQAEEVTSAHFTKVVARSHGLLAWTLYAAPLVVLGALWLFGAAISARIKEYEV"
     gene            695..>1732
                     /gene="pstS"
                     /locus_tag="PKW73_15685"
     CDS             695..>1732
                     /gene="pstS"
                     /locus_tag="PKW73_15685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HNA74790.1"
                     /translation="MNRYRYLQSPCCILALALSLSLSGCGSSSDETESLEPAAKKVEV
                     ANVTVAFQSLQRFDASTRQDEVKLKGAGASFPEPLYQALFKSLRENSKTDVSYEAVGS
                     SAGTVLVQNKQVNFGASDAPLTDEQMKSDANGAILHIPTAFGAVSVVYNLPGVNAVLK
                     LSAEQLALIYLGRITYWDDERLVAGNAELKGVHERIISVHRADGSGSTFALTEFLAEG
                     SGDWNKQMGKGTSMYWPGGMAVTSGGAMVDAVLRSPYAIGYTELNVAVSRNLKSAALK
                     NPSGAYVVPSFKSVREAMEGLAASDSTDLRLDVINSDGSGSYPISTATYVLVYQNQDD
                     AKQAQALTQVLW"
BASE COUNT          386 a          454 c          498 g          394 t
ORIGIN      
        1 ataccatgac cagttttgtc tatggcggca tgcagctttc caccgccaag gtcgacacct
       61 ttgcttccat taccaaactt ctggatgctc gggctgccgt atacagcgtc aatgcttcct
      121 taaatcggca attgctggat aaggctttgc gcacggagca ccgtaagaat ttgactgcca
      181 gcctgacgga actggtcgga gacctcaatg atgggcagtg gcaggaactt gagaagaacc
      241 tgagcgcgga taaaaggcca gccgctgatg ctaagggcag tcttgtgcag gctgccgcca
      301 ccttcgattt cgaggatgaa tttgtggtgc tgcagagggt ctttcaggag ttaaggcagt
      361 tccgtcagac cattgatcag gtgcacacgc ttctggatgc cggtaaggcg gaggaggctt
      421 tgaagctttc catcggcttg aatgtcggac agggtcgcta tctgttcgac aaattggacg
      481 cttctttgag ccaggcagaa gaggtaacct ccgctcactt tactaaggtg gtcgctcgca
      541 gccacggact attggcctgg acgctctatg ccgcaccgct ggtggtgctg ggtgcgctct
      601 ggctcttcgg tgcggcgatt tccgcccgca taaaggaata tgaggtctaa tcatgttccc
      661 tatcaaatta acgtcaacca gtcagagaac aaatatgaat cgatatcgat acttgcaatc
      721 cccctgttgc atcctggcgc tggcactttc cctcagcctc tcgggttgcg gctccagctc
      781 cgatgagacg gagagcctgg agcctgccgc caagaaagta gaagtggcca atgtaaccgt
      841 tgcctttcaa tccttgcaga gattcgatgc ctcgacccgc caggacgagg ttaagctgaa
      901 gggggccggt gcttccttcc ctgagccgct ctatcaagca ctgtttaaga gcctgcgtga
      961 gaacagcaaa accgatgtca gttatgaggc ggtgggcagt agcgccggta cagtgctggt
     1021 gcaaaataaa caagtcaact tcggcgcttc cgatgctccg ctcacagacg agcagatgaa
     1081 gtccgacgcc aatggtgcga tactgcacat accaactgct tttggtgcgg tgtcggttgt
     1141 ctacaatctg cccggtgtca acgccgtgct caagctttcg gctgagcaac tggctttgat
     1201 atacctgggg cgcatcactt actgggatga tgagcgcctg gtagccggca atgcagaact
     1261 caagggtgtg cacgagcgaa tcatctcggt gcaccgggcg gacggctccg gatcgacctt
     1321 tgccttgacc gagtttctgg cggaaggcag cggagactgg aataagcaaa tgggtaaggg
     1381 gacttccatg tattggcccg ggggaatggc ggtaacttcc ggtggtgcca tggtcgacgc
     1441 cgtgctcaga agcccctatg ccatcggcta cacggaattg aatgtggcgg tcagccgcaa
     1501 tctcaaatct gctgccctga agaatcctag cggcgcgtat gtggtgcctt ctttcaaaag
     1561 cgtaagggaa gccatggagg gcctggccgc atccgattcc acggacttgc gtctggatgt
     1621 gatcaacagt gacggcagtg gcagctatcc catttcaacc gccacctatg ttctggtcta
     1681 tcaaaatcaa gatgatgcga aacaagctca agccctgacg caggtactat gg
//
LOCUS       DAOQNZ010000997         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51000_length_1731_cov_3.818616, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000997 DAOQNZ010000000
VERSION     DAOQNZ010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51000_length_1731_cov_3.818616"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="PKW73_15690"
     CDS             <1..1715
                     /locus_tag="PKW73_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNA74791.1"
                     /translation="TATIALQEQYMNKDIPALQEILPFEFSAVLLKGRGNYLGMRRFH
                     DYILEQAVDEEFVDWVNGTMTGDISELDFVPPLDVWMEINSDSDDCMRNRCPNFGSCF
                     YFDAKRQAEKADLIVVNHALLLADAASKGSILPPYQYLIVDEAHHLPDVATDAFSLSI
                     SNRGVKRMLARAAKKVNAPAHLLNEIELAAGQFFFHLNMQAKQPRMRLHEPVEGTEDF
                     HTALEELRHWLEECDFSHILDVEMAREKAQLKAKSIISTVSSYTKCLALLGDPSQDYV
                     TWLERTERSDVKLEVVSAPLDPSSYIADFVMAKPSLISSVWMSATLATAGDDPFNFFK
                     KSIGADRYVVQRQVASPFDFENQALLYLPRDFPEPNHPDFLPYAMEEIERLIELSEGR
                     AFVLFTSKYALSIAYEELSQRLPYECRRQGEMPRQRLIEWFKSTPHAVLFGTASFWEG
                     VSIDGNQLSCVIIDRIPFQVPDDPVYEARCQQMQEDPERSWFNDLALPYATMRLKQGV
                     GRLIRTKRDRGIVAILDNRLTKKYYGRKILECLPPMRIIRALPTPASGVMQDFLDLGM
                     DYNA"
BASE COUNT          389 a          456 c          440 g          446 t
ORIGIN      
        1 ccactgccac catagccctg caggaacagt atatgaacaa ggatataccg gccctgcagg
       61 agatcttgcc cttcgagttc agtgccgtct tgctcaaagg tcgcggtaat tatctcggga
      121 tgcgccgttt ccacgactat attttggaac aggccgttga tgaggaattt gtcgactggg
      181 tcaatggcac tatgaccggt gatatttccg agcttgattt tgtaccgccg cttgatgtct
      241 ggatggagat taactccgat tccgatgact gtatgcgcaa tcgttgcccc aatttcggaa
      301 gctgtttcta ttttgatgcc aaacggcagg ccgagaaggc cgatttgatt gtggtgaatc
      361 atgctctgct tctggctgat gccgcctcca aaggaagtat tctgcctcct taccaatatt
      421 tgatcgtaga cgaggcccat catttgcctg atgtcgctac cgatgccttc agtctttcca
      481 taagcaatcg cggtgtcaag cgaatgttgg cgcgggctgc taaaaaggtg aatgctccgg
      541 ctcatctact aaatgaaatt gaactcgccg ccggtcagtt tttcttccat ctcaatatgc
      601 aggcaaagca gccgcgcatg cgcttgcacg agccggtgga gggcaccgaa gactttcata
      661 cggcgctgga ggaactgagg cattggctgg aagaatgcga tttctcgcac attctggatg
      721 tggaaatggc ccgggaaaag gcgcagctta aggctaagag tatcatcagc actgtctctt
      781 cctacacaaa gtgcctggct ctgcttggtg atccatccca ggattatgtc acctggttgg
      841 aacgcacaga gcgctccgac gtaaaactgg aagtggtctc cgcaccgctt gatccatcct
      901 cctatattgc cgattttgtc atggctaagc ccagcttgat ttccagcgtt tggatgtcag
      961 ccacccttgc caccgccggt gatgatccgt tcaacttttt caagaagtcc attggagccg
     1021 atcgctatgt tgtacagcgc caggtagcca gccccttcga ctttgaaaat caagccctgc
     1081 tttacctgcc ccgtgatttt ccagagccca accatcccga cttcttgccc tatgccatgg
     1141 aagaaatcga aaggctgatt gagctatcag aggggcgcgc ctttgtcttg ttcacgagca
     1201 agtatgctct ttccattgcc tatgaagagt tatcgcagcg cttgccttat gagtgcaggc
     1261 gtcagggaga gatgcccagg caaaggttaa tagaatggtt taagtctaca ccccatgctg
     1321 tcttattcgg tactgccagt ttctgggaag gcgtttccat agacggcaat cagttgagtt
     1381 gtgtcattat cgatagaatt cctttccagg ttcccgatga tcctgtgtac gaagcccgct
     1441 gtcagcagat gcaggaagac ccggagcgat cctggttcaa cgatctggcc ctgccctatg
     1501 ccaccatgcg tttgaagcaa ggtgtggggc gactcatccg caccaaacga gatcgcggca
     1561 ttgtggccat actggataat cgtttgacca aaaagtacta tggacgcaag atcctggaat
     1621 gcctgccgcc catgcgcatt attcgcgcct tgcccacgcc tgcttccggc gtaatgcaag
     1681 actttctcga cctgggcatg gactacaacg cctgaatcga attactctga g
//
LOCUS       DAOQNZ010000998         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51054_length_1730_cov_3.757612, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000998 DAOQNZ010000000
VERSION     DAOQNZ010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51054_length_1730_cov_3.757612"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PKW73_15695"
     CDS             <1..857
                     /locus_tag="PKW73_15695"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNA74792.1"
                     /translation="AELAPADGKIYALRDVTGTVESIPLIAASVMSKKLAAGANLIIL
                     DVKCGKGAFMETEEKAKELAATMASVGRLLKRPVLAVVTDMEQPLGFAVGHTVEVIEA
                     IETLKGNGPDDLNELCMELGALALVEAGKASDKEQAQAILKELISSGKALNAFAKLIE
                     AQGGDSRVLTDYSLMPHAKHQKAYLVPGQAGAPKWLEHIDGRKVAEAAKVMGAGRVKK
                     GDPVDLGVGIILQAKCGDALKAGDAAAIIHYDTDSQYEAAAAKLAEAFTFVDKAVAKS
                     KLIKASIA"
     gene            865..1698
                     /locus_tag="PKW73_15700"
     CDS             865..1698
                     /locus_tag="PKW73_15700"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HNA74793.1"
                     /translation="MEPVKFPHGAAGFRLIPYDVFDPVTNMAIDEALLELHCQGKTPP
                     TLRFYGWEPASISFGYSQRVSPDTVARIKAQGLGVVRRPTGGRAVLHEGELTYSFIGS
                     SNQSEIYGGPPLSSSIQQAYKQICQAFIVGLNRIDVPVELGRADSSYKNHEDCFEATT
                     LADLHYQGKKLVGSAQLRRKEALLQHGSVLIDQGQNRMAELLGLKTRAATREGAECAQ
                     DKRHACLNDIVKVDRTTLEEAIGYGFNKVFDCKFTVYDLSFEEWEVVDALKSNLERYR
                     L"
BASE COUNT          435 a          485 c          476 g          334 t
ORIGIN      
        1 ctgctgagtt ggctcccgcc gacggcaaaa tttatgctct gcgcgatgtc acaggcacag
       61 tggaatccat tcccctcatt gccgcttcgg tcatgtcgaa gaaactggct gccggagcca
      121 atttgattat tctcgacgtc aaatgcggca aaggcgcctt catggaaacg gaagagaaag
      181 ccaaagaact ggccgccacc atggccagtg tcggacgctt gctcaagcgc ccagtgctcg
      241 cggttgtaac cgatatggaa cagccgctgg gcttcgccgt ggggcacacc gtggaagtaa
      301 tcgaagctat cgaaacactc aagggcaatg gccccgacga cctgaacgag ctttgcatgg
      361 aactgggcgc cctggccctt gtggaagcag gcaaggctag cgataaagag caagcccagg
      421 caattctaaa agagctaatc agctccggca aagccctcaa tgccttcgcc aaactcatcg
      481 aggcccaggg tggagacagc cgtgtcttga ccgactacag cctcatgccc catgctaaac
      541 accagaaagc ctatctggtg cccggtcagg ccggagcgcc caaatggctc gagcatatcg
      601 acggccgcaa ggtggcggag gccgccaaag tcatgggcgc cggtcgggtc aagaaaggcg
      661 atccggtaga tcttggcgtc ggcataatcc tgcaggccaa gtgcggcgat gccctcaagg
      721 caggcgatgc ggcagccatt attcactacg atacggacag tcagtacgaa gctgcggcag
      781 ccaaactggc agaggctttc acctttgtcg acaaagctgt agcgaagtcc aaactgatca
      841 aagcatccat agcctgagaa caacatggaa ccggtaaaat ttccccatgg tgccgcaggc
      901 ttcagactca tcccttacga tgtctttgat ccggtcacca atatggccat tgacgaagcc
      961 cttctggagt tgcactgcca gggcaaaacg ccgcccacct tgcgctttta tggatgggaa
     1021 ccggcttcca taagctttgg ttattcccag cgggtctcac cggatacggt ggcaagaata
     1081 aaggcacagg ggctgggcgt ggtgcgcaga cccacgggcg ggcgcgccgt cttgcacgaa
     1141 ggggagctga cctacagctt catcggctcc agcaatcaat ccgagatcta cggcgggccg
     1201 cccctcagct cgtccattca gcaagcctac aaacaaatct gccaggcctt catagtgggt
     1261 ttaaacagga tagacgtacc ggtggaactg ggacgagccg atagttctta caaaaatcac
     1321 gaggattgct tcgaggctac cacactggcc gacctgcact accagggcaa aaagctggtc
     1381 ggctcagctc agcttcggcg caaggaagca cttttacagc acggttcggt gctaatagat
     1441 cagggacaga accgcatggc cgaactgctt ggtttaaaga ccagagctgc caccagggaa
     1501 ggtgccgagt gtgctcagga taaacgtcat gcctgcctca acgacatcgt aaaagtagac
     1561 cgcactacac ttgaagaagc catcggttac ggcttcaaca aggttttcga ttgcaagttc
     1621 acggtttacg acctctcctt tgaggagtgg gaagtagtag acgccctcaa gtcaaatctg
     1681 gaacgctaca ggctctagac tttcaaaacc ggttttcaaa agtagctttc
//
LOCUS       DAOQNZ010000999         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51115_length_1729_cov_3.646953, whole genome shotgun sequence.
ACCESSION   DAOQNZ010000999 DAOQNZ010000000
VERSION     DAOQNZ010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51115_length_1729_cov_3.646953"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1729)
                     /locus_tag="PKW73_15705"
     CDS             complement(<1..>1729)
                     /locus_tag="PKW73_15705"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNA74794.1"
                     /translation="LTTGSAELQCADVLHALPPLDPAQAREHLADLVRIYLEGQRHPL
                     PLFPKTSFGYAQALRKSGNPAQALKTARSQFEDNRNNPGEGSDRKNSVLALTMTRPSN
                     KKEFEGERARSTFNAQTQGGVKKKGVVFFFFGVVLPVLAVLFETKYHFCAERYFDPFP
                     TAGHVVLFLLIPFSNFLIWLATRRDLSQHYGFMSLASGMAMGIGILYTLMFLPLTPMS
                     CLYSLALGFGLLGLAPLLSLPCSFASGKTITKLAHRKDTYFDAHQVEHIGHLIVLVMV
                     VAVELPSTLTRVHLAEAINGSPVLLRRHGLPNPKFAAAADWLSRSGALAYWFSDPSRA
                     DGAEILPAFPEYLSAMDWLRRYGNQEVLLRACYERSGKATDILGSLYESAHPISVEDA
                     RQIYYQVTGKPYNSVPIPASFRATIKHAHAVSESDPLNSGVTDEFDRDTDIAGENVSG
                     MARGLSMSNSLMGGDVDVDGAQARLNWSFQFRNASKYDREARMRLQLPSGAVVSKALL
                     TVNGVTKVAQIQLKSEARAHYQQAVLQHQDPLLVSSCGLDQVLVQCFPVAPGETMQVR
                     LFIDAPCAID"
BASE COUNT          427 a          478 c          494 g          330 t
ORIGIN      
        1 catcaattgc gcaaggagca tcaataaaca aacgcacctg catggtctca cccggagcaa
       61 cggggaagca ctgcacaagc acctggtcaa gaccacagct actgaccaga agcgggtcct
      121 gatgctgcag tacggcctgc tgataatgag cccgcgcctc gctcttgagc tgaatctggg
      181 ccactttggt gacgccgttc accgtcagca aggcttttga aaccacagcc ccggacggca
      241 gttgcaagcg catccgcgct tcccgatcat acttggatgc atttctgaac tggaaactcc
      301 aattcaacct ggcctgggca ccatctacat cgacgtcgcc gcccatgagc gagttcgaca
      361 tggagagccc gcgagccata ccgctgacat tctcaccggc aatatcggta tcgcgatcga
      421 attcatcggt gacaccggag ttgagcgggt cagactcgga aacggcatgg gcatgcttga
      481 tggtagcccg gaaggaagcc gggatgggca cagaattata aggcttgccg gtgacctggt
      541 aataaatttg ccgggcgtct tccacagata taggatgagc actttcgtat agagagccca
      601 gtatgtccgt ggctttgccg gatcgttcgt agcaggcccg caaaagcact tcctggttac
      661 cgtaacgcct gagccaatcc atggccgaca ggtactctgg aaaagccggt agaatttcag
      721 caccatccgc tctagatggg tcagaaaacc aataggccaa ggcgcccgaa cggctcagcc
      781 agtcggcagc agcggcgaac ttgggattag ggagcccgtg tcttctaagc agcaccggtg
      841 agccattgat agcttcagcc agatgcactc tggtcaaggt ggagggcaat tccaccgcca
      901 ccaccatcac aagcacaatc aaatgcccaa tgtgctctac ctggtgagca tcgaaatagg
      961 tatccttgcg atgggccaac ttagtgatgg tcttgcccga ggcaaaactg caaggtaaag
     1021 acaaaagcgg cgccagcccc agcaagccaa agccgagagc aagactgtag agacagctca
     1081 tgggcgtcag gggcaaaaac atcagcgtat aaagtatgcc gatgcccatg gccatgccgc
     1141 ttgccagcga cataaaacca tagtgctgag aaaggtcacg ccgggtggca agccagatga
     1201 gaaagttgga aaaggggatg agcagaaaga gcactacatg accggcagtc ggaaagggat
     1261 caaaatagcg ctcggcgcaa aagtgatact tagtctcgaa gagcaccgca agaaccggca
     1321 agaccacccc gaaaaagaag aaaaccacgc ccttcttttt gaccccgccc tgggtctgag
     1381 cattaaaagt agaacgagct cgctcgcctt cgaactcttt tttgttactg ggtctggtca
     1441 tggtcaaagc cagaaccgaa ttcttgcggt cgctgccttc gccgggattg ttgcggttgt
     1501 cctcgaactg gcttctggcg gtcttcagcg cctgtgcggg gttgccgctc ttgcgcagtg
     1561 cctgcgcata gccgaacgag gtcttgggga agagcggcag cgggtgacgc tgtccctcga
     1621 ggtaaatccg taccagatcg gcaaggtgtt cgcgtgcctg cgccggatcg agcggcggca
     1681 gtgcgtgcag tacatcggcg cattgcagtt ctgcgctgcc ggtcgtcag
//
LOCUS       DAOQNZ010001000         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51366_length_1725_cov_4.518563, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001000 DAOQNZ010000000
VERSION     DAOQNZ010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51366_length_1725_cov_4.518563"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /gene="truB"
                     /locus_tag="PKW73_15710"
     CDS             complement(<1..645)
                     /gene="truB"
                     /locus_tag="PKW73_15710"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNA74795.1"
                     /translation="MKSKTEHFGFLCLDKPGGMTSHDVVAKARRRLGVKQIGHAGTLD
                     PMATGVMVLAIGKATRLLRFLRDDKTYEAKILLGRSTDTDDIEGALLEEAPLPDNLSR
                     EGAEAQLNAFRGKIEQLPPLYSAIHVNGERLYDLARTGKVSPDELSQYVKKRSVEIFE
                     LKLVNWEPPYLDVMVHCSAGTYIRSIARDLGQALGSGACLAALRRTGSGNFPIDA"
     gene            complement(635..985)
                     /gene="rbfA"
                     /locus_tag="PKW73_15715"
     CDS             complement(635..985)
                     /gene="rbfA"
                     /locus_tag="PKW73_15715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNA74796.1"
                     /translation="MSAERALRVSQSIKRELSEMIRRDLKDPRLTGLISVTEVECTSD
                     LRSTKAFISVFGDVNREAVVTALNEQAGFIRGELCRRLKLRFAPELVFKLDDSLERGS
                     KVSELLGKISRGEV"
     gene            complement(1014..>1725)
                     /locus_tag="PKW73_15720"
     CDS             complement(1014..>1725)
                     /locus_tag="PKW73_15720"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74797.1"
                     /translation="MLAEARRLTETKAQHHVTLESLHDLLESGEVKELNIIVKADVQG
                     TAEAIADSIRKLSSSEVQTRVLRTASGDISENDVNLAASSNAIIVGFNVQPDQNGARV
                     AEANGVDIRTYTIIYQITDDITSAVQGLLKPIRQEIQIGQAEVRQIFKVGKGLMIAGC
                     MVLSGKVQRSSIARIERDGQVIYEGKLDTLKRFKDDAKEVAQGFECGMSFEKFSDLQV
                     GDKINSFIIQETKRDASN"
BASE COUNT          355 a          455 c          469 g          446 t
ORIGIN      
        1 ggcatcaata ggaaaattgc cggagccggt gcgacgcaaa gcagccaggc aggcgccgct
       61 tcccagtgcc tgtccgagat cgcgagcaat ggagcgaata taggttccag ccgagcaatg
      121 aaccatgacg tccaggtaag gcggctccca attgaccagc ttgagctcga agatctccac
      181 gctgcgtttt ttgacatatt gagacagctc atccgggctt accttgccgg ttctggctag
      241 atcgtaaagt ctttcgccgt tgacatggat ggcgctatac agtggtggca gctgttcaat
      301 tttgccgcga aaggcgttta attgtgcctc cgctccttcc cggctgaggt tgtccggcag
      361 gggcgcctct tccagcaagg caccttcaat atcgtcggta tcggttgaac ggccaagcag
      421 aatttttgct tcataggttt tatcatccct gagaaaacgc agcagtctgg tggctttgcc
      481 tatggccagg accattactc ccgtggccat cggatcgaga gtaccggcgt ggccgatttg
      541 tttaacgccg agacgacgtc tggctttagc caccacgtca tgggaggtca tacctcctgg
      601 tttgtccagg caaagaaagc cgaaatgctc tgttttagac ttcaccgcgg gaaatcttgc
      661 cgagcaattc ggaaaccttg gaacctcttt ccagcgagtc atccagtttg aagaccagct
      721 ccggtgcaaa gcgcaacttg aggcggcggc aaagctcgcc tcgaataaag ccggcttgtt
      781 cattcaaggc tgttaccacc gcttctctat ttacgtcgcc gaagacggag atgaaagcct
      841 tggttgaacg cagatcgctt gtacactcca cttcggtcac ggaaatgaga ccggtcaagc
      901 gagggtcctt gagatcgcgg cggatcatct ccgaaagttc tcttttaatt gactgagata
      961 ctctcagggc tcgttctgcg gacatgaatt cgtacctgtt taactgccag agtctagttg
     1021 gaagcgtcgc gttttgtttc ttgaatgatg aaggagttga tcttgtctcc cacttgcagg
     1081 tcggagaatt tctcgaaact cataccgcat tcaaagccct gcgccacttc tttggcgtcg
     1141 tctttgaagc gcttgagcgt gtcgagcttg ccttcgtaga ttacctgtcc atcacgttcg
     1201 atgcgggcaa tcgagcttct ctgtaccttg ccgctcaaga ccatacagcc tgcaatcatg
     1261 aggcctttgc ctaccttgaa gatctgtctg acttccgcct gaccaatctg gatttcttgc
     1321 ctgatcggct tgagcagacc ctggacggca ctggtgatgt cgtcggtgat ctgatagatg
     1381 atggtgtaag tgcgaatatc aactccgttt gcttccgcaa cccgggcacc gttctggtcg
     1441 ggttgtacgt tgaagccgac gatgatggca ttagatgatg cggccaggtt gacgtcgttt
     1501 tcggatatgt caccagaggc ggtgcggagc acgcgggtct gaacttcact ggaagagagc
     1561 ttgcgaatgg agtcggcaat cgcctccgcc gtaccttgta cgtcggcttt gacgattatg
     1621 ttcagttctt ttacttcgcc gctctccaga aggtcatgca gcgattccag cgtgacgtgg
     1681 tgctgggctt tggtttccgt cagacgtctg gcttccgcca gcatc
//
LOCUS       DAOQNZ010001001         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51376_length_1725_cov_3.720359, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001001 DAOQNZ010000000
VERSION     DAOQNZ010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51376_length_1725_cov_3.720359"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="PKW73_15725"
     CDS             <1..1515
                     /locus_tag="PKW73_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA74798.1"
                     /translation="RHFHWLAGSGSMYGFQKVSAMGLEGERLCDQIVSQPGAAAPQDL
                     EKLKALLQELSVQFTGPEESGDTTVNLSKARAVVNSGKQEVLVIDEDQKDLESLRKQV
                     EESGLAFRSARSFATTIAELEKRMPEGLILEIPLPDGDAYELVERVRSMPGGDEMAII
                     IVSKQTGFLDKVRSIHCGADAHFEKPVDMKAMFRRLRYLLDKRYQETPRILSVEDDPD
                     QAAFIRAFLESAGYQVRTCTDPKNFESYMSAFQPDLVLLDVMLPGMTGYELARYIRQD
                     ERHATLPVLFLTTQGQIDARIESARSGGDDHLVKPVPPALLLSSVSSRLERARFLKTL
                     LRRDGLTSLLNHTSFMEQAQTVIAQRRRHAGYTALILLDIDYFRSINERHGYPGGDKV
                     LVALSLLLRRRLRQSDIIGRYAGDELGIIADGLDEAEALHLAGRLLADFAAIQHSTLS
                     HSGFYATCSAGISILDAKNMTVESWLGSAFKALQQAKNAGRNCAIAYHEEAVSR"
BASE COUNT          367 a          467 c          471 g          420 t
ORIGIN      
        1 cggcacttcc actggctggc cggatcaggc agcatgtacg ggtttcagaa agtatcggcc
       61 atgggacttg agggcgagcg tctctgcgat cagattgtca gtcagccggg ggctgccgcc
      121 ccgcaagatc ttgagaaatt gaaggcgctt cttcaggagc tttcggtgca gttcaccgga
      181 ccggaagagt ccggcgacac caccgtcaat ttgagcaaag ctcgggccgt ggtcaattcc
      241 ggcaagcaag aagtgctggt catcgatgaa gatcaaaaag acctggaaag tttgcgcaag
      301 caagtggagg aaagcggtct tgccttccgc tcagcccgct cttttgccac cactattgcc
      361 gaactggaaa agcgcatgcc ggaaggactg atcttagaga ttccgctgcc ggacggcgat
      421 gcctacgaat tggtggagcg ggtgcgctcc atgccgggtg gtgacgagat ggccatcatc
      481 atagtttcca aacaaaccgg cttcctggac aaggtgcgct ccatacactg cggcgccgat
      541 gcccattttg aaaagccggt ggacatgaag gccatgttca ggcgcttgcg ttatttgctc
      601 gataaacgtt atcaggaaac gccgcgtatt ctctcggtgg aagacgatcc agaccaggcg
      661 gcatttatca gagcctttct ggaatcggcc ggctatcagg tgcgaacctg tactgatccc
      721 aaaaatttcg aatcatatat gtcggctttt cagcccgatc tggttctgct cgatgttatg
      781 ttgccgggca tgaccggtta tgaattggct cgttatatca ggcaggatga gcgtcatgcc
      841 accttgccgg tgctcttcct caccacccag gggcaaatcg atgcccgcat agagagcgct
      901 agatccggtg gtgatgatca cctggtcaag ccggtgcccc cggccttgct tttgagttcg
      961 gtcagttctc gcctggagcg ggcccgtttc ctgaaaaccc ttttgcgccg ggacggactt
     1021 acttctcttc tgaaccatac ttccttcatg gaacaggctc aaacagtgat tgcccagcgc
     1081 cgtcgccatg ccggctacac tgccttgatt ttgctcgaca tcgattactt ccgttccata
     1141 aacgaaagac acggttaccc cggcggcgac aaggtgcttg tggctctctc tttgctattg
     1201 cgcaggcgtc taaggcagtc ggacatcatc ggtcgctatg ccggcgatga gctaggcatc
     1261 attgccgatg gtctggatga ggctgaagcc ctgcatcttg ccggtcgcct cctggccgac
     1321 tttgctgcca ttcaacattc caccctttct cattccggtt tctatgccac ttgttcggcc
     1381 ggtatctcca tactggatgc taaaaatatg acggtggagt cgtggcttgg ttcagccttt
     1441 aaagccttgc aacaagccaa aaatgccgga cgtaattgtg ccattgctta tcacgaagaa
     1501 gctgtcagcc gatagtttcc accgaaatct aaatgtagaa tcgtcataac tgctttggtt
     1561 ccgtcctggg ctttctcacc gctggtagtg gcacgtccgg ctgcgtttcg gtttggcgcg
     1621 ctctcaaaga tctgcgcctt tgctttggag gcagagagaa agaagattac agttttccct
     1681 tggaaacagg ggacggtcga aacaatatgc ccaggcaagc tctac
//
LOCUS       DAOQNZ010001002         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51602_length_1721_cov_4.606843, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001002 DAOQNZ010000000
VERSION     DAOQNZ010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51602_length_1721_cov_4.606843"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKW73_15730"
     CDS             complement(<1..219)
                     /locus_tag="PKW73_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74799.1"
                     /translation="MAFAALELCCQLTVQVGNAFLSRSRSNSLKFDLGTESRLSDFNP
                     ANVLMEEGPMLVPFKSSHSSCCREVRART"
     gene            688..>1721
                     /locus_tag="PKW73_15735"
     CDS             688..>1721
                     /locus_tag="PKW73_15735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNA74800.1"
                     /translation="MRILLVEDSSMASSSSRETLEQLGLDLEHADSLSTAIELVTKED
                     FDGILLDLDLKDSSGIGTLIELREYAHGIPIVIYTQRKERDQALQVIKYGAQDYLLKG
                     EVPETSVLRCLRHSVERNKVELELHKHQERIQAILDNSYDAFISLDSKMRITDWNKQA
                     EETFGRTKAEVLGRNMAIIIPHHLRRQYLRQTEKVFNQSDASILKLSSEITAAHKDGR
                     EFPVEIGFFRVRENTDYSFCAFLRDISERQRFKEELERQVQERTEHLTQQNELLKQFA
                     KIASHDLQEPLRAVQGFANLLADSTRGKLDKDQEEFIGFIMDGTTRMQNLIRAILLHS
                     ESGKVEDGKHA"
BASE COUNT          475 a          468 c          439 g          339 t
ORIGIN      
        1 ggtgcgcgct ctgacctccc tgcaacaact tgaatggctg gacttaaacg gaaccagcat
       61 cggcccttcc tccataagca cattggcagg attgaagtcg cttagacggc tttctgttcc
      121 caaatcgaac ttcaaagaat tagacctgct gcgactgaga aaggcattgc ctacctgcac
      181 cgttaactga cagcacaact ccagcgcggc aaatgccata aaaccctccc cattttcgag
      241 agcggctttc agttctacga ctcagtcgta ccggtagtgc aataggccgg cgggatagcc
      301 ctctcaaccg ccagtcaaag ggcgagcgac tggtctgcac caacaaagcc ttacacttac
      361 acggaagggg gcagaatttg ctctccaata cgctttgacg ctacgagcac gctttgttaa
      421 gcggcctgtg tcataatgaa attcccagaa cgtttactct gcgcagcgcg gagccgctcc
      481 caggtaaaca aggaaactct tgcggctgtc cgggcgttat taaccgcctg gaaatcggca
      541 aaaactaact accccaggtc ggaaaggctg tgggtcaatg gtcgggtaaa ccacagcctg
      601 aggctgctgg tgggtttacc agcgaaaaac aggtaacacc ggaaactatc acgcgggttc
      661 gaactgccga gggcgatggg agcggttatg agaattcttc ttgtggaaga cagctccatg
      721 gcgagcagtt catccagaga aacactggaa caacttggac tcgacctgga acacgcagac
      781 agtctatcaa cggcaatcga actggtcacg aaagaagact tcgacggcat attgctcgat
      841 ctggacttga aagacagctc cggtataggc accctcatcg agctaaggga atacgcccat
      901 ggcattccga ttgtaattta cacacagcgt aaagagcgag atcaggctct gcaggtgatc
      961 aagtacggag cacaagatta cctgctcaag ggagaggtgc cggaaacttc ggttttgcgc
     1021 tgcctgcgcc attccgttga acgcaacaaa gtagaactgg aattgcacaa acatcaggag
     1081 cgcatccagg ccatcctcga taactcctac gatgccttca tttccctcga ctccaaaatg
     1141 agaatcaccg actggaacaa gcaagcggaa gagacctttg gtcgcaccaa agccgaggtt
     1201 ctaggccgca atatggccat catcattccc caccacctgc gaaggcaata cttgaggcaa
     1261 accgaaaaag tcttcaacca gagcgatgcc agcatactga aactgagcag cgagatcact
     1321 gctgcccaca aagatggtcg ggaattccca gtggagatag gcttcttccg ggtgagagag
     1381 aataccgact attcattttg tgctttcctg agagacattt cggagcggca gcgcttcaag
     1441 gaagaattgg aaaggcaggt acaggagcgc accgagcacc tgacccagca gaacgaattg
     1501 ctcaaacaat tcgctaaaat cgcctcccat gacttgcaag aacccttacg ggccgtgcag
     1561 ggcttcgcca acctgctcgc cgacagcacc cgcggcaaac tcgataaaga ccaggaggag
     1621 ttcatcggct tcatcatgga tggcaccacc cgtatgcaaa atctgattag agccatcttg
     1681 ctacattccg agtcgggcaa ggtggaagac gggaagcatg c
//
LOCUS       DAOQNZ010001003         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51610_length_1721_cov_3.995798, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001003 DAOQNZ010000000
VERSION     DAOQNZ010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51610_length_1721_cov_3.995798"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..633
                     /gene="rimI"
                     /locus_tag="PKW73_15740"
     CDS             43..633
                     /gene="rimI"
                     /locus_tag="PKW73_15740"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNA74801.1"
                     /translation="MVSKESKVIDISPLTIGDLEQVMDIEPVAFGSHHWTRQSFMNEL
                     INPSGFYYAAREHENERVLGYSGFWLIGEEAHVTTLAVHPDYRRLYVGERLLINDIIQ
                     ARKVGADWVTLEVRVSNDSAQKLYGKYSFRSLGVRRNYYQDNDEDALVLWTDRLSSPE
                     FLTLFQKRIKEKGLSYQGLEKLFLGDRLEEESQASG"
     gene            779..1660
                     /locus_tag="PKW73_15745"
     CDS             779..1660
                     /locus_tag="PKW73_15745"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNA74802.1"
                     /translation="MRSQGPASITRVKRKSSVGQVVMTMLSVLVLFLSAFTAISVPVS
                     SQRNLAAFLQNRVDAAVLALPEQAEAKVMAVFPGFKHEAPQSRSTNYCPMAPAAVFVG
                     YVLGPYLGVVACFIFVALGLLGPFFGIHPFAAGGGFSYCNEPGFGYLLALIPTAWVAG
                     MITRGRRTTLSQSLAVVAGLSVTHLCGLMYLFGSVLFSYVMNGTSAALAWQPWVFELA
                     RNMTWYPLPYDILGALALIGIGFPFRWLASTLSAPDIGRTDGNSGKSRDGRFVSVDTF
                     ETAQDERFESRERFELI"
BASE COUNT          342 a          446 c          477 g          456 t
ORIGIN      
        1 ccctatattt gcgcggtgct tccattacgc tgaagaaagg cgatggtgtc gaaagagtcg
       61 aaagtcattg atataagccc tctcaccatt ggtgatttag agcaggttat ggatattgag
      121 ccggtggcat tcggctcaca tcattggact cgccagtctt ttatgaatga gcttatcaat
      181 ccttccgggt tttattatgc agccagggag catgagaatg agcgggtgct tggttattca
      241 ggcttctggt tgattggcga ggaagcccat gtcacaaccc tggcggtgca cccggattac
      301 aggcggctct atgtgggtga gcgcctgctc atcaacgaca tcattcaagc ccgcaaggta
      361 ggagccgatt gggtcactct ggaagtgcgg gtttccaatg attcggccca gaagctttac
      421 ggcaaatata gcttccgcag cctgggagtg cgccgcaatt attaccagga taacgatgaa
      481 gatgcgctgg tgctctggac tgatcgcctt tcctcgccgg aatttctcac cctctttcaa
      541 aagcgcatta aggaaaaggg gctttcttat cagggcctgg aaaaactctt tcttggtgac
      601 cgccttgagg aagaatccca ggcttcaggt tagcggatac tcaggagaga ggaatcttat
      661 ttttccggat ttttacctca actgtaaatg cattggatat ctaaggactt gacttgcctt
      721 gagccttaag acgacaatag ggacgccagt aaaatagcag tgctattgag gaattctttt
      781 gcgttctcag ggaccggcat caattactcg tgttaagcgc aagtcctcgg tggggcaggt
      841 tgtgatgacc atgctctcgg tgctggtgct tttcctctcg gcatttaccg ctatcagcgt
      901 gccggtttcc agccagcgga acctggcggc tttcctgcaa aaccgagttg acgccgccgt
      961 gctcgctcta cccgaacagg ccgaagcgaa agtgatggcc gtttttcccg gcttcaagca
     1021 tgaagccccc cagtcccgct ctaccaatta ttgccccatg gctcccgccg ctgttttcgt
     1081 cggctatgtg ctcgggccct acctgggcgt ggtggcctgt ttcattttcg tggcactggg
     1141 tctgctcgga cccttcttcg gaatccatcc ctttgcggcc gggggcggtt tcagctactg
     1201 taatgagccc ggcttcggct acctgctggc actcattccc accgcctggg tggccggtat
     1261 gatcaccagg gggcggcgca ctaccttgag tcagtctctg gctgtggtgg cagggctgag
     1321 tgtcacccac ctgtgcgggc tgatgtatct tttcggcagc gtcttgtttt cctatgtgat
     1381 gaacggcact tcggccgccc tggcctggca gccatgggtt tttgagctgg ctcgcaatat
     1441 gacctggtat ccgctgcctt acgacatact gggcgctctg gcactgatcg gcatcggttt
     1501 cccgtttcgt tggctggctt ctaccctgtc ggctccggat atcggcagga ccgacggcaa
     1561 tagtggtaaa agtagagatg ggcgctttgt tagtgtcgat actttcgaaa cggcacaaga
     1621 cgagcgcttc gaatctcggg aacgctttga actgatctag atagccatta tgcaagccaa
     1681 aatagcctgg tatctcatta tgttgctctt caagagggta c
//
LOCUS       DAOQNZ010001004         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51695_length_1720_cov_2.772973, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001004 DAOQNZ010000000
VERSION     DAOQNZ010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51695_length_1720_cov_2.772973"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PKW73_15750"
     CDS             complement(<1..668)
                     /locus_tag="PKW73_15750"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNA74803.1"
                     /translation="MTVKDRGNTMVRRGSASNNGAAAGRGDALKTAQQRQPVQTTVDY
                     TQTSVSDIFGSNVFNRATMRSLLPKNVYKALVRCLDHGEQLDASMADIVANAMKDWAI
                     ARGATHFTHWFQPMTGLTAEKHDAFLVFSGDEGEANLEFSGKLLIQGEPDASSFPSGG
                     IRSTFEARGYTGWDPTSPAFLMESTNGKTLCIPTVFCSYSGHALDKKTPLLRSMEAIS
                     KQAVR"
     gene            complement(728..1159)
                     /locus_tag="PKW73_15755"
     CDS             complement(728..1159)
                     /locus_tag="PKW73_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNA74804.1"
                     /translation="MTVQANLKAHTKYLWFDTKKRREYIRITDDVAKFLAETGMKEGF
                     ILVSAMHITAGIYVNDWEGGLIQDIDTWLEKLAPENPNYLHHKTGEDNGDAHLKRILI
                     NHQVTVPVTDGKLDLGPWEQIFYAEFDGQRRKRVILKALGI"
     gene            complement(1253..>1720)
                     /locus_tag="PKW73_15760"
     CDS             complement(1253..>1720)
                     /locus_tag="PKW73_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74805.1"
                     /translation="LATMKLSRPVSGTELRLPPLPAPNKADALTFHTHKPGRGAYPGQ
                     DDLLSTRSIGLISSGTEKGIFLGERGQVLDLLRRGAGGSYVPTLRAVVIDESAGTANR
                     LVGTWDRGAKHWLLTERSHLDYSATIEALRNLDTTRAWQALSQIPRVQTGAGF"
BASE COUNT          406 a          453 c          441 g          420 t
ORIGIN      
        1 cgcactgctt gcttggaaat ggcttccatg gagcgcagaa gcggcgtctt cttatcgagg
       61 gcatgacccg agtacgaaca aaaaacggtc ggaatacaga gagtcttacc gttggtgctt
      121 tccatcaaga aagcggggct tgtgggatcc cagccggtgt agccgcgagc ctcgaaggta
      181 gaacgaattc caccggaggg gaagctggat gcatccggct cgccctgaat aagaagctta
      241 ccggagaatt ccagattagc ctcgccttca tcaccgctga agaccaggaa agcatcatgc
      301 ttttcggcgg tcaaaccggt catgggttgg aaccagtggg tgaagtgcgt ggcaccacgg
      361 gcgatggccc aatctttcat ggcgttggcg acaatgtcgg ccatggaagc atcgagttgc
      421 tcgccatgat ctagacagcg taccagggcc ttatagacgt tcttgggcaa gagcgaacgc
      481 atggtggcac gattgaaaac gttgcttccg aatatatcgg agaccgaggt ttgagtgtaa
      541 tcgacagtgg tttgcacagg ttggcgttgt tgcgcagtct tgagggcatc gccacgtcca
      601 gccgcagcgc cgttattaga agcagaacct cgtctcacca tcgtatttcc tctatctttt
      661 actgtcatct agtctttacc tcattttgga aagtagcatt tgaccgattt acggcaagtg
      721 ttcgatatta aatccccagg gctttcaaaa tgacgcgctt gcgcctttga ccatcaaatt
      781 cggcatagaa aatctgttcc cacggtccca gatccagttt gccgtccgtt acaggaacag
      841 ttacttgatg gttgatcaag atccgcttca aatgggcatc gccattatct tcgccggtct
      901 tgtgatgtaa ataattcggg ttttccggag ccagcttctc aagccaggtg tcgatgtctt
      961 gaatcagacc accttcccaa tcattgacgt aaatgccggc tgttatatgc atggcagaaa
     1021 cgagaataaa accctctttc atgcctgttt cggctaaaaa ctttgcgacg tcgtcagtaa
     1081 tgcgaatata ttcgcgtcgc ttcttcgtgt cgaaccacaa atacttggta tgggctttta
     1141 aattcgcctg caccgtcact tatgttctcc tgccccggtt ttgctaatga tagcccaact
     1201 tcagcaaaag actattagat gactcggtta agtaactttg gcgcaacaga aactaaaagc
     1261 cagcccctgt ctgcacccga ggaatctgcg aaagtgcttg ccaggccctg gttgtatcaa
     1321 gattgcgtaa agcttctatt gtggcactgt aatcaagatg actccgctcg gtcaaaagcc
     1381 agtgtttagc ccctctgtcc caggttccca ccaaccgatt ggccgttccc gcgctttcat
     1441 ctatgaccac cgctctcaaa gtcggcacat agcttcctcc ggctccccgg cgcagcaaat
     1501 caagaacctg acctcgctcg cccaggaaga tacccttttc ggtaccggaa gatataagac
     1561 caatcgagcg ggtggaaagc aaatcatcct ggcccggata ggcgccccgt ccgggcttgt
     1621 gagtatggaa ggtcagcgca tcagctttat taggggccgg cagggggggc agacgcagct
     1681 cggtgccgct caccggccga ctgagtttca tagtagccag
//
LOCUS       DAOQNZ010001005         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51705_length_1720_cov_2.255255, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001005 DAOQNZ010000000
VERSION     DAOQNZ010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51705_length_1720_cov_2.255255"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKW73_15765"
     CDS             complement(<1..664)
                     /locus_tag="PKW73_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74806.1"
                     /translation="MKPSIFSQNNRAPKVFRSAVRSILKSGLPCVLAVSVCLGGCTSV
                     SLGGKTKEGQGQTPSQPSTGSAPGNAPNIAGYWKINYTANNRPMTASARITQAGNNFQ
                     GSGSDDHNSAPFTITDGKVTGGGEVRFFKKYTGGKSTPIEYNGKMRMVDAMGYKGPYI
                     EGEYVAAFNGKIIEGKWEAQTSAIADPSPPPTPDPQMPAQQPPTQPMPNNQGPPPDQG
                     PPQ"
     gene            966..>1720
                     /locus_tag="PKW73_15770"
     CDS             966..>1720
                     /locus_tag="PKW73_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74807.1"
                     /translation="MANSTAAAATNNDNAAEDPNKKKGQTADAAESPKEGEEHEEKEQ
                     GAIEKAACNIWQDVSSLVSWGVKKGGELAGDAASLVVPNLWCEAPKKPAEATAATASA
                     GTPEAVQQTDKPKVEAEKPEERGFFGRAWDVVKSGVDAVTGAVTDAWRGIWGKDATIT
                     EDKTSDGQKKGFTVTEKDGDVITATRDGTQIKKGDGTIITREGGVTTVEKNGISISRD
                     RSGRETFKLKDGTTGTIDANKEMSVELKALHET"
BASE COUNT          416 a          416 c          492 g          396 t
ORIGIN      
        1 cctgcggagg tccctggtcg ggcggcggtc cctgattatt cggcatcggc tgagtggggg
       61 gctgttgcgc cggcatttgc gggtccggtg taggtggagg ggacgggtct gcaattgccg
      121 atgtttgcgc ttcccactta ccttcgatta ttttgccgtt gaaggccgct acgtactcgc
      181 cttcgatgta gggtcctttg tagcccatgg catcgaccat gcgcatcttt ccgttgtact
      241 cgatgggcgt cgatttgccg ccggtgtact tcttgaagaa tctgacctcg ccgccacctg
      301 tgaccttgcc gtcagttatg gtgaaggggg cgctgttatg atcgtccgag cccgaaccct
      361 gaaagttgtt gcctgcctgg gttatgcggg cgctggccgt catcggcctg ttgtttgcgg
      421 tgtagtttat cttccaatat ccggcaatat tcggggcatt gcccggagcc gacccggtgc
      481 tgggttgact gggggtctgt ccttgccctt ctttggtttt gccgcccagg gacacactgg
      541 tgcagccacc cagacaaacc gatactgcca ggacgcaggg cagaccactt ttgagaatag
      601 accgaacagc gctgcggaaa acttttggtg cgcggttgtt ttgagaaaag atgctgggtt
      661 tcattcttgt tgaagcctga atagtaagtc ggcaaagcgc aagctggagc gttgttttga
      721 gcgttcaaaa ctctctgatg ctttctagta cgaattctag cgttctttcg ccgggcaaaa
      781 tgcccagaaa tgccctctta aaccgcataa aaggatagaa aatctgtctt ttgggtattt
      841 ttaatgttgg atgggaattc caccatggac actgcacatt taatgtttca aaatactgac
      901 aacttactat gtggccattc gggtcacgtt taactcccag aacctcatag agctccgtcc
      961 aggaaatggc taattccaca gcggctgctg ccacaaataa tgataatgcc gccgaggacc
     1021 ccaacaaaaa gaaggggcag actgctgacg cggctgaaag tcctaaagaa ggcgaggagc
     1081 atgaggagaa agagcaaggc gcaatagaga aggctgcttg caatatctgg caagacgtat
     1141 cctctctggt ctcttggggc gttaagaaag gcggagaact ggccggtgat gctgcttctc
     1201 tggtcgtgcc caacctctgg tgtgaagctc ccaaaaagcc agccgaagct actgctgcaa
     1261 ctgcctctgc aggaacgcct gaagcggttc agcaaactga taaacctaag gtggaagcag
     1321 aaaagcctga agagcgcggt ttcttcggca gagcctggga tgtagtcaaa agcggagtgg
     1381 acgcagttac tggagcagtg actgatgcct ggagaggtat ctggggtaaa gatgccacca
     1441 tcactgaaga caaaacttct gacggacaga agaagggatt taccgttact gaaaaagacg
     1501 gtgacgtgat tactgccacc agagacggca cccagataaa gaagggtgac ggcactatca
     1561 tcacccgcga aggcggtgtc accacagtcg agaaaaatgg tatttctatc agccgtgacc
     1621 gcagcggcag agaaacgttc aaattaaagg atggcacgac cggaacaatc gacgccaaca
     1681 aagagatgag cgtagagctg aaggctcttc atgaaaccat
//
LOCUS       DAOQNZ010001006         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51724_length_1719_cov_4.045673, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001006 DAOQNZ010000000
VERSION     DAOQNZ010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51724_length_1719_cov_4.045673"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1577)
                     /locus_tag="PKW73_15775"
     CDS             complement(204..1577)
                     /locus_tag="PKW73_15775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74808.1"
                     /translation="MGNLKTRDRFEFLSVSRNLALNALGLTLCLSAGAVFVDLDGQSS
                     GAAFAQKLPKASREQGGPAGAAKADPTAAKNTHEKQSDLAVPAPDTAVSSDGTEIPPV
                     DGTNGRAGGDATTGGAVSEPYIGTAGNGATGGDGSIEAPSDGAGAQSGAPAAVDAAAS
                     AQSQALTPEQEAANEKAMLQNEAVDHYEAARAYLHNLDFDLADIELRASIMCMPHIRA
                     SHRDYALVSLLRGHPGRALAEFMIVVGLGDAIPYSDEQKEAIKNDGLKLHYRQGLALA
                     AQSKWADSIVEFEWAKFYRPNAAKVTRSIAFAYASEGKFDLAEKQYAESFAIDPSDAY
                     GHADFAFLLAAKGSAERAVEQLNEAVKLQPKVAALHVDLGWMAESKGDLAKAESEFQE
                     AIKLAPRQATLWTHLGKLQARQGKSELAMQAYKEALAIDPQQLDASEGLQRLDGSAGK
                     KKEGSKS"
BASE COUNT          394 a          523 c          432 g          370 t
ORIGIN      
        1 ttttgccgcc cgcctctagt ccctctttcc gcccagcgtc aagccgacgg tcttgaaccg
       61 tggaagcctc tgcattatcg ccgggagaag ccaaattaga cctgccgaaa agaaaaccaa
      121 caattcatct ctaactggat gagatcttgg cctgatatta ggcgaagcca acccccatgt
      181 cattggggga attacgatgc ccctcagctc ttcgagcctt ccttcttctt gcccgcagac
      241 ccgtccaggc gctgcaagcc ttcgctggcg tccaactgtt gaggatcgat agccagagcc
      301 tctttgtagg cctgcatggc cagctcggac ttaccctggc gagcctgtaa tttgcccagg
      361 tgggtccaga gcgttgcctg ccggggcgcc agtttaatcg cttcctgaaa ctcgctctcg
      421 gccttagcca gatcgccctt cgattctgcc atccagccca gatccacatg cagagccgcc
      481 accttgggct gcagtttcac cgcttcattg agctgctcca cggcacgctc ggcactgcct
      541 ttggcagcca gcaagaaggc gaaatcggcg tgaccataag catcggaagg atcgatggca
      601 aagctttcgg catactgttt ctcagccaga tcgaacttac cttcgctggc ataggcaaag
      661 gcgatggagc gcgttacctt ggcggcattg ggacggtaaa acttggccca ttcaaattcg
      721 acaatggaat cagcccactt ggactgagcc gctaaagcca agccctggcg ataatgcagc
      781 ttaagtccat catttttaat agcctctttc tgctcatcgg aatagggaat ggcatccccc
      841 aaaccaacca caatcatgaa ttcggccagg gctcgcccgg gatgccccct gagcaaagac
      901 accagcgcat aatccctgtg agaggcgcga atatgcggca tgcacatgat ggaagcgcgc
      961 aattcaatat cggcaagatc aaaatctaga ttatggagat aagcacgggc cgcttcgtaa
     1021 tgatcgacgg cttcattttg cagcatcgcc ttttcgtttg ccgcttcctg ctccggcgtc
     1081 aacgcctgcg actgggcaga agcagcagcg tccaccgcgg caggcgcgcc agactgtgca
     1141 ccggcgccat cagacggcgc ttcaatgctg ccgtcgccgc cggtagcgcc attaccggca
     1201 gtaccaatat aaggctcgct taccgctccg ccggtagtgg catcgccacc ggcacggcca
     1261 ttggtgccat caacaggtgg aatctccgtg ccatcactgg aaacagcagt gtcaggagcc
     1321 ggaacggcca gatcggattg cttctcatgt gtgtttttcg cagcggtggg atcggctttt
     1381 gcagccccgg caggtccacc ctgctcacga gaagcttttg gtaatttctg ggcgaaagcg
     1441 gcaccggaag actgcccgtc aagatccaca aagacagccc cagcagacaa acacaaggtt
     1501 agacccaaag cattgagggc caggttcctg gaaaccgaca gaaattcaaa cctgtctcta
     1561 gttttcaaat ttcccatgcg gaagctcccc ttgctacttc aattttgaaa gtcacaagtc
     1621 ctattatgcc atcatccagg gcaggcagag caacaccagc aaacctgact ctatcccagg
     1681 gtttccccca gctttttaat attgcccgac tatacggac
//
LOCUS       DAOQNZ010001007         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51811_length_1718_cov_2.685508, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001007 DAOQNZ010000000
VERSION     DAOQNZ010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51811_length_1718_cov_2.685508"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKW73_15780"
     CDS             complement(<1..296)
                     /locus_tag="PKW73_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74809.1"
                     /translation="MEPETLEGIDHLTTGGIYSVYIKSDEEGRARFYLTKIVALEVNV
                     VHIVVYHDDFLTRPTVENVPLSSVCFEGDMDIDEARSRHLAVSRRLFSLMRPVY"
     gene            589..1641
                     /locus_tag="PKW73_15785"
     CDS             589..1641
                     /locus_tag="PKW73_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74810.1"
                     /translation="MQAYNSTQNFNSTKEDFATSGGGGISSSGARRLKTVNDRWSGAS
                     SIAAVALDTARRVREVAEARSDALLAIEENELAREIQAARKALETLPPNSPERSLQIR
                     RMEQARMKLCTDRPASYTKAIKLVARYALLEEQRLKIISSSSEHMAVAQMAVTSTESM
                     MDRLPHSFKGKHTSLPERTPEDMSPSRRLTELRRHLEERAKSLDETRNHSLHNMAASH
                     PILKEIIEKLEREELPGDGTLIIMSTRGHYLAPTGSMSPHFIEVSRLKVGGVSADGGG
                     FNRFSQDLEHISGAIIMRPIYDEFGQAMTGPGKTTNGKPVFRKMVVETIGQVPAEITH
                     GLDFLQILRRFKPPMK"
BASE COUNT          484 a          463 c          453 g          318 t
ORIGIN      
        1 aagtagaccg gacgcatcaa actgaagagc cggcgagaga cagccagatg tctggagcgc
       61 gcctcatcta tatccatatc cccctcaaaa cagacggagg acaggggtac attttcgacg
      121 gtggggcggg tcaggaaatc atcatggtag acgacaatat gaaccacatt gacttccaga
      181 gcgacaatct tggtcaggta gaagcgagcc cgcccctctt cgtcggactt tatatagacg
      241 ctatagatac caccggtcgt caggtggtca attccttcta aagtctctgg ttccacgggg
      301 caaacatcac tcagaagcgt ttctagggag caagggcatt atagaaaatc ccaggacata
      361 tagatagaac gctgagataa cttgtggtat tccccgcaga agaccggaac ccttaccgag
      421 cggcggcttt cagcccagat taactaaaat gtgggattta tacggtctta tggggatttt
      481 ccccaatcga ctgcaaccct gcaagagaga ataatcgggt ttagttcgac tttcagttaa
      541 atagcacccc ttagcgaggg tcgcccgcaa agcgcgatcg aagcaatcat gcaagcatat
      601 aattctactc aaaacttcaa ctcgaccaag gaagacttcg ctacatccgg cggtggcggc
      661 atcagttctt cgggcgctcg caggctcaaa acagtaaacg acaggtggtc cggagctagc
      721 tccatagcgg cagtggcact ggatacggcc agaagggtga gagaggtggc ggaagcaaga
      781 tccgatgctc tcttagccat cgaagaaaac gagctggcca gggagattca agcagcgcgc
      841 aaagcccttg aaacgttacc gccaaactcc ccggaacgca gcctgcaaat caggcgtatg
      901 gaacaggccc ggatgaaact ctgcaccgac agaccggcat cctacaccaa agcgatcaag
      961 ttagtggctc gctacgccct gctggaagag cagcgcctca aaataatcag cagttccagc
     1021 gaacacatgg ccgtggccca gatggccgtc acttccaccg agtccatgat ggaccgactg
     1081 ccccattcct tcaaaggcaa gcacaccagt ctgccggaga ggacaccgga agacatgagc
     1141 ccatcgcggc gcctgaccga actgaggcgg cacctggaag aaagagccaa gagcctggat
     1201 gaaacgagaa atcacagcct gcataacatg gccgcttctc atcccatctt gaaagaaatc
     1261 atcgaaaaat tggaaagaga ggaactgccg ggagacggca ccttgatcat aatgtccacg
     1321 agagggcact atctagcccc gacaggcagt atgtctccgc acttcatcga ggtctcaaga
     1381 ctgaaagtgg gcggcgtcag cgccgacggg ggcgggttta accgcttctc gcaagacctg
     1441 gagcatatca gcggcgccat aatcatgcgc ccaatctacg atgaattcgg gcaggccatg
     1501 acgggtcctg gtaaaacaac caacggcaag ccggtcttcc gcaaaatggt agtggaaaca
     1561 atcggtcagg tgccggcaga aataacccat gggctcgact tcctgcaaat actgcggcgt
     1621 tttaagccac ccatgaagtg accgtaagaa gcgaaaatag aactaagaga caggcatctt
     1681 caagtcaggc aatgactgct gcctgtctct tttgccta
//
LOCUS       DAOQNZ010001008         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51917_length_1716_cov_3.826008, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001008 DAOQNZ010000000
VERSION     DAOQNZ010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51917_length_1716_cov_3.826008"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKW73_15790"
     CDS             complement(<1..547)
                     /locus_tag="PKW73_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNA74811.1"
                     /translation="MVENLLAFNGNVVGKGQLIEISQGKPIVPDRPVIPFIAGDGIGQ
                     EITNAMREVVNRAVGVTYGRKRKIAWLETYAGEKAMEIFGSALPLDTLKAIELFRVAI
                     KGPLNTPTGQGLRSLNVALRSHFDLYQCVRPLRHFVGVPSPMVRPQDLNVVIFRENTE
                     DVYAGIEYQQGSDKALQLAAFL"
     gene            complement(645..1400)
                     /locus_tag="PKW73_15795"
     CDS             complement(645..1400)
                     /locus_tag="PKW73_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74812.1"
                     /translation="MNKLALIPITIFPANGSLISAHSALVSALEEAKTARGLFHAVES
                     DMRTEQDTEAAADEGSFRRRRHPNRLNILYYAAKLRLTMAWQDLIQKRAELVKSIVHL
                     RYAVDESTKSNVEAQKDAARINVIFAENLSLTAYRYIAAVEKTLSYGLNLNELYVEGK
                     PVNLSAIVGEEYSRLTREWLVQSSGHGSEYRFDEGDGSAVPVRDYYLTVDEATHDAIS
                     WEVSADTIGGRAQLYNVSTGDVKAPGQRPQCFD"
     gene            complement(1502..>1716)
                     /locus_tag="PKW73_15800"
     CDS             complement(1502..>1716)
                     /locus_tag="PKW73_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74813.1"
                     /translation="GAVVATVTLTGGEFFYDDVYAATARSLHLFAATRLILTTDLNAP
                     AGPGYILLLPQPPDASIIGKVKVTAD"
BASE COUNT          397 a          437 c          475 g          407 t
ORIGIN      
        1 caaggaaggc cgctaactgc aaagccttat cagagccttg ctgatactcg attccggcgt
       61 agacatcttc ggtgttttcc cgaaaaatga caacgtttaa atcttgcggt cgcaccattg
      121 gcgatggcac accgacaaag tggcgcaatg gccgaacgca ttgatagagg tcaaaatggc
      181 tgcggagagc gacattgaga ctacggagcc cttgcccggt gggcgtattg agtggtcctt
      241 tgatggccac gcgaaagagt tcgatagctt ttaacgtatc aaggggcagc gcactgccga
      301 aaatctccat agccttttca ccggcatagg tttccagcca ggctatcttt ctcttgcgcc
      361 cataagtaac tccaacagct cggttgacca cctcgcgcat ggcgttagtg atttcctggc
      421 cgataccgtc tccggctatg aagggaatta cggggcgatc aggaacaatg ggtttcccct
      481 gtgatatttc gatgagttgg cctttgccca caacgtttcc gttgaaagcc agtaggtttt
      541 ctaccatagc aaattcctgt gttgttgatg aagggttagt gaacgtgcag ttcgaaccat
      601 tgcgaatcac tattgaactg ggcttccggc ctgccgcaag gggttcagtc aaagcactga
      661 ggtctttgcc ccggtgcctt aacatctccg gtggagacgt tgtagagttg ggctcgacca
      721 ccgatggtgt cagcggagac ttcccaactt atggcgtcgt gggtggcttc atccacggtc
      781 aggtagtagt ctctgaccgg cacggccgac ccgtcaccct cgtcaaagcg gtattccgag
      841 ccatgcccgc ttgattgcac cagccattca cgagtgaggc gagaatactc ttcccctacg
      901 atggcagaga ggttgacggg cttgccttcg acataaagct cgttcaagtt aagtccgtaa
      961 gaaagcgtct tttccacggc cgcaatatac cggtaagcgg tgaggcttag attttcagcg
     1021 aaaatgacat tgatgcgggc cgcgtccttt tgcgcctcga cattggactt cgtgctttcg
     1081 tccacggcgt aacgcaggtg cacgatagac tttaccagtt ccgctcgctt ctggatcagg
     1141 tcctgccagg ccatagtgag gcgcagcttg gcagcgtaat agagaatatt cagccgattg
     1201 gggtgacggc gtcgacggaa agagccctcg tcagcagcgg cttcggtatc ctgttcggtc
     1261 cgcatgtcgc tttcgactgc atgaaaaagc ccacgagctg ttttagcttc ctccagagcc
     1321 gatacaaggg cactgtgggc actgataaga ctgccattgg caggaaaaat cgtaatcggg
     1381 ataagagcta atttgttcat aattctcctt gagcgatgtc gctaaagagg cgcgagtaca
     1441 gagaagaggc tgccgtactc gcccttgagt gaatgtaaaa tcctttccgg tgaaaagaga
     1501 ctcagtctgc ggtcactttt accttgccga taatggaagc atcgggcggc tgcggtaaaa
     1561 gcaggatgta accaggacct gccggtgcgt tcaagtcggt ggtaaggatc aatcttgtgg
     1621 cggcgaacag atgcaagctg cgtgcagtgg cggcataaac atcatcataa aaaaactcgc
     1681 cgccggtcaa cgttacggtg gcaactacag cgcctt
//
LOCUS       DAOQNZ010001009         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_51934_length_1716_cov_3.169777, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001009 DAOQNZ010000000
VERSION     DAOQNZ010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51934_length_1716_cov_3.169777"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKW73_15805"
     CDS             <1..261
                     /locus_tag="PKW73_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74814.1"
                     /translation="AAMKMENKGLDNGLKNHTRHCSLEPSCSESGYSLYSGGKWFDLD
                     PKGNEMARKFFQASKKEAGHIFKITGVFKRNELRVATISENK"
     gene            262..801
                     /locus_tag="PKW73_15810"
     CDS             262..801
                     /locus_tag="PKW73_15810"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNA74815.1"
                     /translation="MNKLLVPLISLGLALSFASGVRAAKMDKLSLETTAFKAGAAIPT
                     RFTGDGADLSPELRWSAVPKGTVSIAVSCEDPDAPRGTWWHWILYNLPPAMQSLKEGM
                     EKTPNLASGALQGLNDFDKSGYNGPSPPPGSTHRYFFRVYALDSKLNLPAGAKKEAFS
                     KALAGHILAFGEVMGTYKR"
     gene            complement(798..>1716)
                     /locus_tag="PKW73_15815"
     CDS             complement(798..>1716)
                     /locus_tag="PKW73_15815"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA74816.1"
                     /translation="MQKDALEKLNTKSMLTEPELLSTLTPDVNLVATLAALSSAMPAK
                     SKATARLVVQKVADELTRKLANPMRQALTGALNRARRTSRPRLSEMDWHRTINANLKH
                     YQPDLKTIIPERKIGFSRKRSSLKDIIVLVDQSGSMATSVVYASIFSAVLASIAAVST
                     KMVVFDTAVVDLTEKLADPVDVIFGTQLGGGTDINGALGYAETLISRPSETILVLISD
                     LYEGGNGNELKKKLYRIAGSGVRLIALLALNDEGAPAYDHNMAAYMAELGAPAFACTP
                     DQFPDLMAVAIAKGDLADWAASQGIAAGR"
BASE COUNT          373 a          433 c          504 g          406 t
ORIGIN      
        1 gcggccatga agatggaaaa taagggtctg gacaatggtt tgaagaatca caccaggcac
       61 tgttcccttg aaccttcttg ttccgagtcc ggctactctt tgtacagcgg cggcaaatgg
      121 ttcgaccttg atcccaaggg caacgaaatg gccagaaaat tctttcaggc aagtaagaaa
      181 gaagccggac acattttcaa gattaccggc gtcttcaaac gaaatgaact gagggttgcc
      241 acaatatcgg agaataagta gatgaacaaa ctgctggtgc ctcttattag tttggggctg
      301 gctttatctt ttgcctctgg ggtgagggct gcaaaaatgg ataagttgag tctggaaact
      361 actgccttta aagccggcgc cgctattcct accagattca ccggggatgg tgccgacctt
      421 tcgccggaac ttagatggag cgcggtgccc aaaggtacgg tcagtattgc cgtctcttgc
      481 gaagatcccg acgcaccgcg cggtacctgg tggcactgga tactttataa tttgccgcct
      541 gccatgcagt ctctgaaaga gggaatggag aagacgccca accttgcttc cggtgccctg
      601 caagggctca acgattttga taagagcggc tacaacggtc cctctccgcc cccgggctcc
      661 acacacagat attttttccg ggtctacgct cttgatagca agttgaattt gccggccgga
      721 gccaagaaag aagccttctc aaaagccctg gccgggcata ttctggcatt tggcgaagtg
      781 atgggcactt ataaacgtta gcgaccggcg gctatgccct gagaggcggc ccagtcggcc
      841 agatcccctt tggctatggc cacagccatc aggtccggaa attggtccgg ggtgcaggca
      901 aaggccggtg cgcccagttc cgccatgtaa gcggccatgt tgtgatcgta ggccggcgca
      961 ccttcatcgt tgagggccag caaggcaatc agtcttacgc cggagccggc gatgcggtaa
     1021 agctttttct tcagttcatt tccattgccg ccttcataaa ggtcgcttat cagcaccaga
     1081 atggtctcgg aaggacggct gatgagagtt tccgcatagc cgagggcgcc gtttatgtcg
     1141 gtgccgccgc ccaattgggt gccgaaaatg acatccacag ggtcggctag tttctcagtc
     1201 aggtccacta cggcggtatc aaagacgacc atcttggtgg agacggcggc aatggaagcg
     1261 agaactgctg aaaagatgct ggcatagacc actgaggtgg ccatggaacc gctctggtcc
     1321 acgagcacga ttatgtcttt caggctggag cgcttgcggc tgaaacctat tttgcgctct
     1381 ggaataatgg tttttagatc cggttgatag tgcttgagat tggcattgat ggtgcgatgc
     1441 cagtccattt cactgagtct ggggcggctt gtgcgccggg cccggttcag ggcgccggtg
     1501 agggcctgtc gcatggggtt ggctaacttt ctggtcagtt catcggctac cttttgcacc
     1561 accagtctgg cagtggcctt gcttttggca ggcatggccg aggaaagggc ggccagtgtg
     1621 gccaccagat tgacatcggg agtgagggta gagagcagtt caggctcggt caacatgctc
     1681 ttcgtgttga gcttttccag agcgtctttt tgcatc
//
LOCUS       DAOQNZ010001010         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_52059_length_1714_cov_3.192285, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001010 DAOQNZ010000000
VERSION     DAOQNZ010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52059_length_1714_cov_3.192285"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PKW73_15820"
     CDS             <1..583
                     /locus_tag="PKW73_15820"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNA74817.1"
                     /translation="SVIMANSNGSQGSEKAETDQIASVVGSERVTTLFGKEAAINNLE
                     EQSRGKSVVHFSSRLPLLESNPLRSVLPILSDSTAEGAQSKVTVDKLCGTKMASDLIV
                     WSASSVNSKDVRGNAVKVFSRGLNYAGARNVLMSLWSQPESERIDELVNFYKSKQSGL
                     GPAQSLRKAQLAALSRDRAPRTWAAFQLLGPGN"
     gene            827..>1714
                     /locus_tag="PKW73_15825"
     CDS             827..>1714
                     /locus_tag="PKW73_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74818.1"
                     /translation="MSTALVAQMVLLPLPVLAQEAAPSWNDTQSELTLDDKPKDADGS
                     GKTIAVKAKDKEVSQPAPAETTSAATTNGGGETFVIDTDKTEESEKKPEATAATGLNP
                     AVTLSPVEAPLSNAPTENLSAGSVFGGTTGSLLQSATPPKLGDSPEAVEVLTEPTVGS
                     QTAVTSAGSSGTFGTADSLPTNEVHQLGASINDTSKDSVLAVELVATGDEPFLIDLDE
                     VEQIEKVVEYENLPTDEGKTKIKTGARFPVVVLSQISSKTAKKGDPVEARLKYDLKIG
                     DRLIAKKGSVVRGHINYCLR"
BASE COUNT          417 a          454 c          470 g          373 t
ORIGIN      
        1 ctcagtcatt atggctaatt ccaatggcag tcagggttcc gagaaagccg agaccgatca
       61 gattgccagc gtggtcggca gcgagagagt caccaccctg ttcggcaagg aagcggccat
      121 caacaatctg gaagaacagt caagaggaaa gtctgttgtc cacttctcgt cccgcctgcc
      181 ccttctggaa agcaatcccc tgcgctcggt tctgcctatc ttgagcgata gcaccgccga
      241 aggtgcccag agcaaggtca cggtcgacaa gctttgcggc acaaagatgg ccagcgatct
      301 gatcgtctgg agcgccagct cggtcaactc taaagatgtg cgaggcaatg ccgttaaggt
      361 cttcagtcgc ggtttgaact atgccggagc cagaaatgtc ttgatgagtc tctggagcca
      421 gcctgagtcg gagcggattg atgagcttgt taatttctac aagagcaagc agtccggatt
      481 gggaccggcc cagagtttgc gcaaggctca gctggctgct ctctccaggg accgggcgcc
      541 ccgtacctgg gcggccttcc agttgctggg accaggaaac taagcatctc tttctgaaac
      601 agacaggccc tcggttttaa ccgggggcct gttaagtctt aaagcttcgg tagtcaagga
      661 tgttattgac atcgccggac acggtataaa ttccttagct ttatatctag ctaatagatg
      721 attgccggga atcatattta ctatttcttt caacgaggcg ttgatcatta tgggtttgtc
      781 catcaagcat tctcgttcat cttttcccag agcaatgagc atatttttga gcacggcgct
      841 ggtagcgcag atggtgctct tacccttgcc cgtgctggca caagaggctg ctcccagttg
      901 gaatgacacg cagtcggagc ttactctcga cgataagccc aaagatgctg acggcagtgg
      961 taagaccatc gccgtcaaag cgaaagataa ggaagtttcg cagcctgccc ccgccgaaac
     1021 cacaagtgct gccaccacta acggcggcgg cgagactttt gtcatagata cagacaagac
     1081 ggaagagagc gagaagaagc cggaagcgac cgccgccact ggtcttaatc cggcggtgac
     1141 actcagcccc gtggaagcgc ccctcagtaa tgctccgacg gaaaatctca gcgccggctc
     1201 cgttttcggc ggcaccaccg gcagtttatt gcagtcagcc acacctccca aactgggtga
     1261 tagccccgag gccgtggagg tactgaccga acctacggtc ggcagccaga ctgcggttac
     1321 aagtgccggc tccagcggca cttttggtac cgccgattcc ttaccgacaa atgaagtgca
     1381 ccaactgggc gctagcataa acgatacgag caaagacagt gttctggccg tagagttggt
     1441 tgctacgggc gatgagccgt tcctcattga tcttgatgaa gtagagcaaa ttgaaaaggt
     1501 tgtcgaatac gaaaacctgc ccaccgacga gggcaagacc aagatcaaga ccggtgctcg
     1561 cttccccgtg gtagtactgt ctcagatctc ttccaagacg gccaagaaag gcgaccctgt
     1621 ggaagcccgt cttaagtacg atttgaagat aggcgatcgc ttgattgcta aaaagggttc
     1681 ggtggtaaga ggccatatca attattgtct taga
//
LOCUS       DAOQNZ010001011         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_52105_length_1713_cov_3.880579, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001011 DAOQNZ010000000
VERSION     DAOQNZ010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52105_length_1713_cov_3.880579"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PKW73_15830"
     CDS             complement(<1..363)
                     /locus_tag="PKW73_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74819.1"
                     /translation="MALVWNDHLSAQVTLVGMSLDLIGGLYLAYDLLGGDKGPLSTLT
                     RCVTYSVIVIACVGFSMGLKFGLLAGAGLGIALGLHLERIGRGQKETTAFLFALGLVR
                     ALGISAACWFDNLKSVAYC"
     gene            complement(467..1258)
                     /locus_tag="PKW73_15835"
     CDS             complement(467..1258)
                     /locus_tag="PKW73_15835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNA74820.1"
                     /translation="MDAAHAVSVIVEEGNPARSMLSSNISPRKTLVFIWSLAPWVFLA
                     LGLHQFRSIFWSFLLYHGFCLLPAILYNRGHWQKHMHIPTRKDLLVLVFASIGFSAFT
                     FITYSLLSDLLLDRALALESMHERGFKMSWYLPLSLYFLTVNPILEELFWRGVVLNEL
                     CDEGQPVFSLPGIWTNVAFAVWHFLVIRLFVAPVFIPLAIGFVLAVGFFMSWMYRRKD
                     TLILPALWHSIVFDLAVIIILTMVLFGPISAEAAPTIVPLLERLR"
     gene            1319..>1713
                     /locus_tag="PKW73_15840"
     CDS             1319..>1713
                     /locus_tag="PKW73_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74821.1"
                     /translation="MQDYHPTRQMSGHVEPRGNSFRRLTTVLVLAAAFATGAPASKAF
                     STYKWFGGAVHRSIIKEALKDKGLSKQALEEICNGCDAQDDPGGKNFSVSQYHACDNK
                     ITETRQFLEQVLNQCAEEAKTCENSATARR"
BASE COUNT          502 a          470 c          445 g          296 t
ORIGIN      
        1 gcagtaagcg acgctcttca agttatcgaa ccagcaggcg gctgaaatac ccagggcccg
       61 caccagtccc agggcaaaga ggaaggccgt ggtctctttc tgcccccgcc cgattctttc
      121 cagatgcagc cccaaggcga tgcccaggcc ggcgccggcc agaagtccga acttgagccc
      181 catggagaat ccgacacagg caatgacaat tacactgtag gtgacgcaac gggtgagggt
      241 gctgagcggc cccttgtcgc cgcccaataa gtcgtaagcc agatagagcc cgccgatcaa
      301 atcaagactc atgcccacca gggtcacctg ggcgctcaga tgatcgttcc agaccagtgc
      361 catcagtact tttcctatgc ctccatggga acgctcacag gccgctatga gcccccttcc
      421 cagttgcgct catactctca tgcagggcgg ggaaaaatca acaactctag cgcaaacgct
      481 ctaaaagcgg cacaatagtg ggcgccgctt cggcggaaat gggtccgaac aagaccattg
      541 tcaaaataat tatcacggcc agatcaaaaa caatggaatg ccagagcgcc ggcaaaatca
      601 aggtatcttt gcgcctgtac atccacgaca taaagaaccc cacggccagt acaaagccaa
      661 tagccagcgg aatgaagacc ggcgcaacaa acaaccggat cacaagaaaa tgccagacgg
      721 cgaaggccac attggtccag ataccgggca aactgaagac cggctgacct tcatcacaga
      781 gttcgttcaa gaccacaccg cgccagaaca gttcttccag aatcggattg acagtgagga
      841 aataaaggct gagcggcaaa taccagctca ttttgaagcc gcgctcgtgc atactttcaa
      901 gggccagggc gcggtccaga agcaaatcgc tgagcaagct ataggtgatg aaagtgaagg
      961 cggaaaagcc tatggaggca aacaccagca ccagaaggtc tttcctggtc ggaatgtgca
     1021 tatgcttctg ccagtgtccc cgattgtaga gaatggccgg tagaaggcaa aagccgtggt
     1081 aaagcaaaaa ggaccagaag atactgcgaa actggtgcag gccaagtgca agaaagaccc
     1141 agggagccag ggaccagata aagacaagag ttttgcgtgg gcttatattg gaagacagca
     1201 ttgaacgagc cgggttacct tcctctacaa taaccgaaac agcatgggcc gcgtccaaga
     1261 aaaatgcagt actgtaactg tctatggaat tatatataca aggcaaaacc gccggataat
     1321 gcaagactat cacccgacca gacagatgag cggtcatgtt gagccacgag gaaattcttt
     1381 ccggcggctt acgacggttc tcgttctagc ggctgccttt gcaaccggcg ccccggcaag
     1441 caaggccttt tccacctaca aatggttcgg cggcgccgta cacagaagca tcatcaagga
     1501 agctctcaag gataagggac tgagcaaaca agcgctggaa gaaatctgca atggttgcga
     1561 cgcacaggac gacccgggcg gcaagaattt ttcagtttct cagtaccatg cctgcgacaa
     1621 caaaattact gaaaccaggc agttcctcga acaagtttta aaccagtgcg ctgaagaagc
     1681 aaaaacctgc gaaaactccg ctaccgcccg ccg
//
LOCUS       DAOQNZ010001012         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_52119_length_1713_cov_3.225573, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001012 DAOQNZ010000000
VERSION     DAOQNZ010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52119_length_1713_cov_3.225573"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKW73_15845"
     CDS             complement(<1..539)
                     /locus_tag="PKW73_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74822.1"
                     /translation="MIKYDQEKLDVDGLPEYIRDTDSPARDAFGSISTRSLAEREAKR
                     LSAVSERQSQKDTGGFANIFSDVQTPPADELEALWPGVHQDFFQSHAPKRTPSFHMMV
                     GFLAGAVVSMVCIWGYSAISGLIASGSHSANKAAVTAPATTAATAQPAPSSVPQGVDP
                     SAVLKPAHPTVEVQNGDTMA"
     gene            complement(1010..>1713)
                     /locus_tag="PKW73_15850"
     CDS             complement(1010..>1713)
                     /locus_tag="PKW73_15850"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNA74823.1"
                     /translation="KKVAVQHSPRRLLKNSIIVEKKQDAMVTLGKLVDTGLGAADYPL
                     LLLSDCALTSHPIFSRFAQKLSGETGLMSSLSLEDLASDVESMPGTTLWTLDIPVVNN
                     MMPGMVKSIQNELKKFSRQGLSESEYAEVRLYMNGALPVRWMANSHLAARSILESVVL
                     CGQSDPLPELYAGIKGSSTESLNKFVRERFKPDRASLVIAGTKQVVGQVHGIRHDEPS
                     GERSGDKLEKSTEAK"
BASE COUNT          447 a          463 c          426 g          377 t
ORIGIN      
        1 gccatggtat cgccgttttg cacttctaca gtaggatgcg ccggcttgag aacagcactg
       61 ggatcaacac cttgcggtac cgaagatggt gcgggctggg ccgtggcagc ggtggtggca
      121 ggggcggtga cggctgcctt gttagcacta tggctgccgg aggcaatcaa accggagatc
      181 gcggaataac cccaaataca aaccatggaa acaacggcgc cagccagaaa acccaccatc
      241 atgtggaagc taggggtacg cttcggtgca tgggattgaa agaagtcttg atgcacaccc
      301 ggccagagag cctcgagctc atcggctggt ggtgtctgaa cgtcagagaa gatattggca
      361 aagccgcccg tatctttctg agactgacgc tcgctaacag cggacaggcg cttcgcctca
      421 cgctcggcca gggagcgggt agatatcgaa ccgaatgcat cgcgagcggg tgagtcggta
      481 tctctgatat attcaggcag cccgtctaca tccagtttct cttggtcgta cttgatcact
      541 gtgctcatct cttgcaacgc cactggggta acactctcag catattatcc ccattgtcac
      601 tagaaagtca aacttcttca tgccaaccca aaacccttac tgagcaatca tttcaggatt
      661 ccgcaccagt ttcatgagac ggtcaatcaa ggcgagagcc agcattctag cggatcatat
      721 taattaattg acaacctcgc ccaagtttac aatgctccct taagcagcga ttacagccac
      781 tttcggcaag cagcaagagc aggcaccggc gcagcccacg ggcaatttcc ccgactgccg
      841 gacaaagaaa agcaccatac tctgcataga ccatcgacaa cagacgagag tggttacgca
      901 tatacccgat ttgcggggat tgtgagaggg ggctaattgt aaagggagca aacctcttga
      961 taactacttt tttctcacat taaagtcgca agaggttttc aacaggtacc tacttcgcct
     1021 cggttgattt ctccagctta tcgccggagc gctccccaga aggctcatca tgcctgatgc
     1081 cgtgcacctg cccaacaacc tgcttggtac cggcaataac aagggaagcg cgatccggct
     1141 tgaagcgctc tctcacaaat ttgtttagac tttcggtgga agaacctttg atgccggcat
     1201 aaagctcggg taaaggatca gactgaccgc aaagcacaac cgattcaaga atactgcgag
     1261 cggccaaatg actattggcc atccagcgca ccggaagagc accattcata tataggcgta
     1321 cttctgcgta ttcactctcg ctcaagccct gcctggaaaa cttcttcaac tcattctgta
     1381 tagacttcac catccccggc atcatattgt tcaccacagg tatatcgagg gtccagagag
     1441 tggtgcccgg catactttca acatctgaag ccaggtcttc cagagataga ctactcatca
     1501 aaccggtttc accggacaac ttctgggcaa agcgggaaaa aatagggtgg ctggtgagag
     1561 cacagtcact gagcaagagc agtggataat cggcagcacc aagaccggta tcaacaagct
     1621 taccgagagt aaccatggca tcctgcttct tctctacaat aatagagttc ttgagcaacc
     1681 gccttgggct atgctgaacc gctactttct tgg
//
LOCUS       DAOQNZ010001013         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_52241_length_1711_cov_3.174517, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001013 DAOQNZ010000000
VERSION     DAOQNZ010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52241_length_1711_cov_3.174517"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PKW73_15855"
     CDS             <1..1224
                     /locus_tag="PKW73_15855"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA74824.1"
                     /translation="RGETTREGLSYSLGAAYYCDDEGATAKLVAEMGLKPQAFGGDKN
                     DFFFRQKTYNEADKSLQNLVYQGMKDLEPIIRPVKDLDLSINDNFSQALTMDKGKFKD
                     LLTPLSPAFRTFLDSHLKSSICGTSEDVSIISGSWLLQDLFQKSYVLPGGNPALGRAL
                     RNALSSGGHRIRTEAFVWSVRVDESGATVVYENKGQLKQVRCRHLIMATPPMVTGRIL
                     SGVANECKGPLFAFRYGSYLVANLIFDEEVFQGTYDNFRALSPECGITDVIKAETPYV
                     KEGTYQKSMGSVLTVYRPWSPGTEGREKLAAGDRSMFAREILSGVEIQAAEGGRPLAG
                     NWRQHLKEVILSRWGHAIIVPTPGYFQRLAALARANQSPVYSLAHSSLAGIQCLESAV
                     IAGERAARRALSGKG"
     gene            1446..>1711
                     /locus_tag="PKW73_15860"
     CDS             1446..>1711
                     /locus_tag="PKW73_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74825.1"
                     /translation="MSLLTAVGLAFAQVPAQGAPASKGKNSQPVYGLSNQQTLSSALG
                     AWGVPAAVLVGGKRVRGFYVTGLTGSGIAADLSLNSGDVVLTLAG"
BASE COUNT          421 a          462 c          489 g          339 t
ORIGIN      
        1 cggggcgaaa ctacacgaga gggactctcc tactccctcg gtgctgcata ttactgcgac
       61 gacgagggcg ccacagcaaa gctggtagcc gaaatgggcc tgaaacctca ggcttttggc
      121 ggcgacaaga acgacttttt ctttcggcaa aagacctaca acgaagcaga caagagtctg
      181 caaaatcttg tttatcaggg aatgaaagac ctggagccca tcataaggcc agtcaaagat
      241 cttgacctgt ccatcaatga caacttcagc caggctctga caatggacaa gggtaagttc
      301 aaagatttgc ttaccccctt atcgcctgca ttcaggactt ttctcgacag ccacctgaag
      361 tcatctattt gcggcacaag cgaagacgtc tcgataattt caggttcctg gctcttgcag
      421 gatctttttc aaaaaagcta tgtgttgccc ggcggcaatc cagctcttgg tcgggctcta
      481 cgaaatgcgt tgagcagcgg tggtcaccga atcaggaccg aagcttttgt ctggtcggtc
      541 agggtggacg aaagcggcgc tacggtggtc tacgagaaca agggacaact caagcaggtg
      601 cgctgtcgcc atctgataat ggccacacca cccatggtga cgggtcgaat tttgagcggc
      661 gtggcaaacg aatgcaaagg gccgctcttt gcctttcgct acggttctta cctggtagca
      721 aaccttatct ttgacgagga agttttccag ggcacttacg acaacttcag ggctctgagc
      781 ccggaatgcg gcatcactga tgtcatcaaa gcagaaactc cctatgtgaa agagggcacc
      841 tatcaaaaat ccatgggctc ggtgctaact gtttatagac cctggagccc cggtacggaa
      901 ggcagggaaa aactggcggc aggcgatcgc agcatgtttg cacgagaaat attgagcggc
      961 gttgagatac aggccgctga aggtgggcgc ccactagccg gcaactggcg gcagcacctg
     1021 aaggaagtga ttctgagccg ctggggtcat gccatcatag tgcctacgcc cggctacttt
     1081 cagcgcctgg cggccctcgc ccgagcgaat cagagcccgg tctacagcct ggcccactcc
     1141 agcctggccg gcattcaatg cctggaaagc gctgtcattg caggggagcg cgccgcgaga
     1201 agagcgctgt ccggcaaagg ctaacatctt agagaaaccc gttcgggagc aaagaaaaag
     1261 caacgatgag ggcaaaactg tctaaaatat aggcagcaat cgagacagca ggagataagt
     1321 tggtggagcc tggataccgg ttaagcgacc cccgcaggga tttgagcggc aagagtgcaa
     1381 gagaacctcg cccgctcgaa gacaagactg cagtccgccg gagcggccgc cacttggtct
     1441 gcctgatgtc tctactgacc gcagtcgggc ttgcctttgc gcaagtgccc gcccagggag
     1501 caccggcatc caaaggcaag aacagccagc ctgtctacgg actttccaat cagcaaaccc
     1561 tttcaagtgc actcggagcc tggggagtac cagccgccgt gctggtaggc ggcaagcgag
     1621 tgagaggatt ttatgtgacc ggtctgaccg gctccggtat cgcagccgat ctcagcctta
     1681 acagcggcga tgtagtgctt actctggcag g
//
LOCUS       DAOQNZ010001014         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_52378_length_1709_cov_3.665659, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001014 DAOQNZ010000000
VERSION     DAOQNZ010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52378_length_1709_cov_3.665659"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKW73_15865"
     CDS             complement(<1..243)
                     /locus_tag="PKW73_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74826.1"
                     /translation="MYKPKKYVRLDKLPKTPRLPDIRNLLNEAKKARACTVELPWRSE
                     KLGLPFSLTVRVEVGGGEPIWTLYEGEGNSSRVMWST"
     gene            466..1281
                     /gene="proC"
                     /locus_tag="PKW73_15870"
     CDS             466..1281
                     /gene="proC"
                     /locus_tag="PKW73_15870"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNA74827.1"
                     /translation="MLEGKKLAVIGVGKLGEALISGLLKNGALKAEDVCGTVGRENSI
                     SRVREKLPIAVSLDNKEAVKGKDLILLAVKPQNMAKVLLEIKDVIEDHQLLISVAASV
                     STAFVEEKLGRPAPVVRAMPNTPSVINAGMTGLCGGSQAKPEHLKACEAIFRCVGETV
                     FVDESLMDAVTALSASGPAYLYVVIESLAEAGVKLGIPRETSTLLAAQTMYGAAKMVL
                     ESKAHPALLKDTVTTPAGCTIDGLMELEEGKLRVTLIKAVVKAAERARELVHR"
     gene            complement(1289..>1709)
                     /locus_tag="PKW73_15875"
     CDS             complement(1289..>1709)
                     /locus_tag="PKW73_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74828.1"
                     /translation="KARGTFYLPRGKKLQLELAKDRRVDLALLKRFPTGYLHSIDGEG
                     SKITDDDAEKIASVTGLKELDLSGTPLSSKGVEHLRSLKSLEKLWLDNTLIDDTCVPA
                     LISLSTLKKLSLEGTSLNEIARESLKKDLPESTELKI"
BASE COUNT          427 a          445 c          464 g          373 t
ORIGIN      
        1 ggtgctccac attaccctgg agctattacc ctcgccttcg taaagagtcc agataggctc
       61 gccgccccct acctccactc tcaccgtaag agagaagggc agtccaagct tctcggaacg
      121 ccagggcaat tcaactgtgc aagcccgggc cttcttggcc tcattgagca ggtttctgat
      181 atcaggaagt cgcggcgtct tcggtaactt gtcgagacgg acgtacttct tgggtttgta
      241 cattgtcaaa cctgaccact gggaaagggg gaaatagaag ctatcaaaaa gaaattatct
      301 ttcaacttac cttcccgatc aagggaagaa ctaaccttac cactgaaggc aagatttccc
      361 tggattcaag caagcgtaat ggcaagccct gggccaagag cgtgttaatg ttgataggct
      421 agaggttctg gctcttggcg caaaaggcag gaaggtaaga aaaatatgct ggaaggcaaa
      481 aaactggctg ttatcggtgt gggtaaactg ggagaagctc ttatctcagg gcttctcaag
      541 aacggagcgc tgaaggcgga agatgtctgc ggcacagtgg gccgggaaaa ttccatcagt
      601 cgagtaagag agaagctacc tatcgcagtc tcccttgaca ataaagaggc tgtcaaaggc
      661 aaggacctca tcctcctggc tgttaagccg caaaatatgg ccaaggtttt gcttgagatc
      721 aaagatgtca ttgaagacca ccagcttctc atctccgtgg cagcctccgt ttccacagcc
      781 ttcgtggaag agaagttggg cagaccggcg ccggtggtaa gagccatgcc caacacgccc
      841 tccgtcatca atgccggcat gaccgggctc tgcggcggct cgcaggccaa acccgaacac
      901 ttgaaagcct gcgaagcaat ttttcgctgc gtgggcgaga ccgtatttgt agacgagtcg
      961 ctcatggacg ccgtcaccgc tctatcggcc agcggtcccg cttatctgta cgtggtgatt
     1021 gagtctctag cggaagcggg agtgaaacta ggcatcccca gagaaacctc gaccctgcta
     1081 gcggctcaga ctatgtacgg cgccgccaaa atggtgctgg aatcgaaggc tcacccggcc
     1141 ctgctcaagg atactgtgac aaccccggcc ggctgcacca ttgacggact gatggagctg
     1201 gaagaaggta aactgcgcgt caccctgatt aaagccgtag tgaaagcagc ggagcgagcc
     1261 cgagagctgg tgcaccgcta gcttccactt aaattttcaa ttccgtgctc tcaggcaaat
     1321 ctttcttgag cgattctctg gcaatctcat tcaaagaggt gccttccagg gagagctttt
     1381 tgagggtgga gagggaaatg agcgccggca cgcaagtatc gtcgataagg gtgttgtcca
     1441 gccagagctt ttcgagagac ttcaatgaac gcagatgctc cacccctttg gaagaaagcg
     1501 gtgtgcccga taaatccagc tccttcagtc cagtgacaga ggcgattttc tcagcatcat
     1561 catctgtaat cttgctgcct tctccatcga tagagtgcag gtaaccggtg ggaaagcgct
     1621 tcaatagagc cagatccact cgtctatcct tagcaagctc caactgaagt ttcttgcccc
     1681 ggggaagata aaaggtgcca cgggctttg
//
LOCUS       DAOQNZ010001015         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_52392_length_1709_cov_3.112455, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001015 DAOQNZ010000000
VERSION     DAOQNZ010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52392_length_1709_cov_3.112455"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKW73_15880"
     CDS             complement(<1..290)
                     /locus_tag="PKW73_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74829.1"
                     /translation="MSVRHRLKKLVHCIALVATLAFASPVLAAGGGEGNKMEDTHAKP
                     LSPECGMPMWFYRCKRNFRYPVPTDFEFAYIDRAGNLAITGPFSIAFDFGKDA"
     gene            554..1186
                     /locus_tag="PKW73_15885"
     CDS             554..1186
                     /locus_tag="PKW73_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74830.1"
                     /translation="MNIEETPTGANTGMEIEHPRGVKLVTNPHDTLDAGYGQTTLEKL
                     RRMLDKPSAKQASSGHNLNCLEIIVRQGELAARQNWNIYCHALRAEVNATGEHKSFTA
                     AVHLLDTVNRFLQTERTLFVEALSQQNGNRGVVLRITAHAQNVEERLEELVMMHVDEP
                     DGAFTRGIVRLLRSFDELIEVMHESLSRMSAAELSYLDGRYLCALMGQRS"
     gene            1475..>1709
                     /locus_tag="PKW73_15890"
     CDS             1475..>1709
                     /locus_tag="PKW73_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74831.1"
                     /translation="MAIHIELRDPDWSRQARHIKGSIRPATDNKSARARKRSPAGKGA
                     IELLSKKLSRRLYFLLRYLLAHPAYCGETDQKAP"
BASE COUNT          473 a          448 c          416 g          372 t
ORIGIN      
        1 gcatccttgc caaagtcaaa tgcaatagaa aagggtccgg tgattgccag attgcccgct
       61 ctgtcaatgt aggcgaattc aaagtcagtc ggtaccggat agcgaaaatt tcgcttacag
      121 cggtaaaacc acataggcat cccacattcc ggactgagtg gtttagcatg ggtatcttcc
      181 atcttattcc cctcgccacc accggcggct aaaacaggcg acgcaaatgc gagcgtagct
      241 actaaggcaa tgcagtgaac aagctttttc aggcggtgac gaactgacat ctttcgtcct
      301 taacatacgg atgcacagat gatacctgct tgaaaaggga aatagcccca ggacgggtta
      361 tcctttgcgc caccgcgaaa tctccatggc agccaggcga aaggcagtca ccgccacatt
      421 attttccgcg ccggcaatct tgaaatctgt cataatgcaa acaggttcac ttatggaatt
      481 cttgaacccc tcaaacgaag caggcatcag ccagcaatca tgaatccact ggccggtgcc
      541 aaagacagga tttatgaaca ttgaagaaac gcccaccggc gcaaataccg ggatggaaat
      601 tgaacatccc cggggcgtaa agctggttac aaatccccat gacactcttg atgccggcta
      661 cggccagacg accctggaaa aactcaggcg tatgctggat aaaccctctg ccaagcaagc
      721 ttccagcgga cataatctca actgcctgga gattatagtc aggcaaggcg agctagccgc
      781 caggcaaaac tggaacatct attgtcacgc tctgcgtgct gaagttaatg caaccggaga
      841 gcataaaagt ttcaccgcgg ctgtgcatct gcttgatacg gtcaacagat tcttgcagac
      901 ggagcggacg ctgtttgtgg aagcactcag tcaacaaaac ggcaatcgag gagttgtctt
      961 gagaatcacc gcacacgccc agaatgtcga ggaacgactg gaagaactgg taatgatgca
     1021 cgttgatgag ccggacggag ctttcaccag aggcattgtt cgactgcttc gatcattcga
     1081 cgagctgatc gaagtaatgc acgagagcct gagccgcatg tccgccgctg agttgagcta
     1141 tctggatgga cggtatcttt gcgcgctcat gggccagcgc tcctgattca ggagcaagcg
     1201 cagaattgag agaagctcca cccatggagc ccctctctgc gatatagaat ctgcaacacc
     1261 tgaaaaagcg agaaaaataa aagtctaaag ccaaccgctg cctaattggg gacagcggtg
     1321 ctcagtctcg gcttgctgct ccataacggt ttatcagtcg gaaatgcctt aaaccaactt
     1381 caatgcccat attttgacat tttcctgaac tattctttcc tcatttgata gcttcactta
     1441 ctaattttct aaccaatctc tgagggctct agccatggcc atacatattg aattgcggga
     1501 ccctgactgg tcccggcaag ctcgccatat aaaagggtcg attagaccgg ctacagacaa
     1561 taagagtgcc agagccagga aacgcagccc agcaggcaaa ggcgctattg agttattatc
     1621 aaagaagttg agtcgcagac tgtactttct tctacggtac cttctggccc atcctgccta
     1681 ctgcggcgaa acggaccaga aggcaccca
//
LOCUS       DAOQNZ010001016         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_52674_length_1704_cov_4.272893, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001016 DAOQNZ010000000
VERSION     DAOQNZ010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52674_length_1704_cov_4.272893"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(617..>1704)
                     /locus_tag="PKW73_15895"
     CDS             complement(617..>1704)
                     /locus_tag="PKW73_15895"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74832.1"
                     /translation="AFIASPEIVTAFALAGRLDFNPLTDSLVGADGKEFKLSPPQADA
                     LPPNGFIADAEAIVGTSGDAEQTAVMVSPKSSRLQLLSPFPAWSGADLVGLPVLLKAK
                     GKCTTDHISPAGVEWLRFRGHLDNISNNMFSRVVNSFTGEVGTGINLTNGEKQPLPQI
                     ARSYKEQGLGWVVIGDTNYGEGSSREHAAMSPRYLGGLAVIARSFARIHRSNLWKQGM
                     LALTFVDANDYERFQPGDKLSLLGLQSLAPEVNLQAVITHADGSSETIMVSHGMGDEQ
                     IRWFKEGSFLNFVRTKREKATTAEEAPFCSATDHTNCDHGTVKPTVATAEEKPAAEDK
                     PVVKPKSGFMEKLKSFFRKFLESLGWE"
BASE COUNT          360 a          433 c          512 g          399 t
ORIGIN      
        1 agcactggca ggcaggctcg agcacacctc gaaccggcgg cctaaaccga attcccctgt
       61 ggaattcgaa ttgtttagct gtgcctgaaa gctgggctta tctgtttggg ttttctcttg
      121 gaaaaatctt gaaatgatgc ttcgatgata ggtatgcgcg ccaggctgaa acaagcctct
      181 tgtctggtct tttgcattaa agcttgcctg cggggctatt aaattgtgtt gtaggaattg
      241 cgctagctag caatagcgag agtggaggaa aggtagggcc ggtgctgggt ttggggtgtg
      301 taggcacgca aaaagcggca ccgaatacgg tgtcgcttct gcgttcttca aaaactaacg
      361 ggcttccctg tctggagtga caaggaagat actagagaag gactgtttca aacttgttgc
      421 tgtgtactaa gaaaaatagc tgtttttctt agtgccttta atgccgcatt ggagtgggtt
      481 taacaggatt ggttcgtcgc cggacttaaa gaatagaccg gtcgactgcc ggtgttcaaa
      541 aaaaagagga ggatctcctt attggggaga tcctccatgc aaattacggc agtgtcgtca
      601 ggtcagaagt aaacgattac tcccagccca ggctttccag aaatttgcgg aagaaactct
      661 tgagcttctc cataaatccg gacttcggct tgaccacagg cttgtcttct gcggcaggct
      721 tttcctccgc ggtggcaacg gttggcttga ccgtgccgtg gtcgcagttg gtgtgatcgg
      781 tggcgctaca gaaaggcgct tcttcggcag ttgttgcctt ctcccgcttc gtgcgcacga
      841 agttcaagaa ggaaccttct ttgaaccagc gaatctgttc gtcgcccatg ccgtggctga
      901 ccatgatggt ctcgctgctt ccatcggcgt gagtgatgac ggcttgcaga ttcacttccg
      961 gggcaagcga ctgcaagccc aaaagcgaaa gtttgtcacc aggctggaag cgctcatagt
     1021 cattggcgtc cacgaaggtg agagccagca taccctgctt ccacaggttg cttctgtgaa
     1081 tgcgggcaaa gcttctggca atgacagcca gaccgcccag atagcgcggc gacatggcgg
     1141 cgtgctcgcg gctgctgcct tcaccatagt tggtgtcacc gatgactacc cagcccagac
     1201 cctgctcttt gtaggagcgg gcaatctgcg gcagaggctg cttttcgccg ttggtcaggt
     1261 tgatgcctgt gccgacctca ccggtgaagc tgttgacgac gcggctgaac atattgttgg
     1321 aaatattgtc caggtgcccg cggaagcgca accactccac accggccggg gagatgtgat
     1381 cggtggtgca tttgcccttg gctttgagga gaaccggcaa acccaccagg tccgcaccgc
     1441 tccaggcggg gaacggcgag agcaattgca ggcgcgagct cttgggcgag accatcactg
     1501 ccgtttgctc cgcatcgccg ctggtgccga cgatggcttc cgcatcggcg atgaagccgt
     1561 tcggcggcag agcgtcagct tgaggcgggc tgagcttgaa ttccttgccg tcagcgccga
     1621 caagcgaatc cgtgagcgga ttgaagtcca gtctgccggc cagggcgaaa gcggtgacga
     1681 tttccggact ggcaatgaag gcgt
//
LOCUS       DAOQNZ010001017         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_52824_length_1702_cov_3.003643, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001017 DAOQNZ010000000
VERSION     DAOQNZ010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52824_length_1702_cov_3.003643"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PKW73_15900"
     CDS             complement(<1..780)
                     /locus_tag="PKW73_15900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNA74833.1"
                     /translation="MQTIVNPNKTAIRTKLVITDVDGTLSSFWDYFVPAIRDFVREMS
                     QEVKVSVDELSQDIGHVIERRGTHEYPWLLEETSFAWKHYKDKVDEFTGRIVQPFWEA
                     LDSNREKYLRPFPTVLDTLAALKSQGIKVVALSDAPDFMARVRNQQLFDGLLDAVYAL
                     ETREPSKNDIFQPITFEYGRKRLSSLLNSSSNIKSSFFTLPQSYEKPCPHGLDRILED
                     FDVYPQECMFIGDSLSKDGIVAASRGIRFIWAHYGHHVPAEY"
     gene            962..>1702
                     /locus_tag="PKW73_15905"
     CDS             962..>1702
                     /locus_tag="PKW73_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74834.1"
                     /translation="MASFGSFLALALQIGAPFYLTAGNIKTPAATAAEARPEPENSGA
                     HGKSPVKAEAEATLGAAHGEVALNGSIDSSEDSQALIWQVEESSPGLFRVLKGSQFRV
                     RLDQELTSDESHDGQPVAASLVDELKAGPVVLGRAGAQVLGIVNDVSHSRRTIKAYLP
                     GKHWLDAQGRIGFNFSGIVDPSSNLIKIDAVPSEKTSVNQVKKTRAQLVANKDGEYVV
                     KYAVGKYAAMDLAIGTIGLATGPVGILTS"
BASE COUNT          384 a          409 c          472 g          437 t
ORIGIN      
        1 gtattcggcc gggacatggt gtccatagtg ggcccagatg aagcgtatgc cgcgcgatgc
       61 tgccacaatg ccgtctttac tgaggctgtc accaatgaac atgcattcct gcggataaac
      121 atcgaagtct tccagaatgc gatccagacc gtggggacag ggtttctcgt aagactgagg
      181 cagggtgaag aagctggatt tgatgttgct gctggagttg agcagggagg agaggcgctt
      241 cctgccgtat tcgaaagtaa tcggctggaa tatgtcgttc ttgcttggtt ctctcgtttc
      301 cagggcataa acggcgtcca gcagaccatc aaagagctgc tgattgcgta cccgggccat
      361 gaaatcagga gcgtcgctta aggctaccac cttgatgccc tggcttttca aagctgccag
      421 cgtatcgagc acggtaggga aggggcgcag gtacttctcc cggttgctgt ccagggcttc
      481 ccagaagggc tgcactatgc gtccggtgaa ctcgtccact ttatctttat agtgtttcca
      541 ggcgaagctg gtctcttcga ggagccacgg gtattcgtgc gtgccacggc gttcgatgac
      601 gtggcctatg tcctgggaca gttcgtccac ggaaactttg acctcctggc tcatctctcg
      661 cacaaaatcg cggatggcag gaacaaagta gtcccagaat gatgaaaggg taccgtctac
      721 gtcggtgatg accaacttgg tgcgtattgc tgttttgttt ggattgacaa tggtctgcat
      781 gattcttttc tctatcttct gtcttgcttt tggtttctat tttcttagtt gctatccaat
      841 cagcttgact taaaatgggt aaacatcaag ttaagatgcc ggttcgcgca aaacttattc
      901 atcaaacttc ttgaatacgc aaaaaaacat gactgtttac aaacctggaa atgcaatctg
      961 tgtggcgtct ttcggttcct tcctggcttt ggctttgcaa atcggcgcac ctttttactt
     1021 aacagccggg aacataaaaa ctcccgctgc aaccgctgca gaagctagac ccgagcctga
     1081 aaatagcggc gctcatggta aaagtccggt gaaggcggaa gcggaagcta ctctcggagc
     1141 cgcccatgga gaggttgcgc taaatggttc tatcgattct tccgaagatt ctcaagccct
     1201 tatctggcag gtagaagaga gttcacccgg tcttttcagg gttctaaaag gtagccagtt
     1261 tcgggtccgt cttgaccagg aactgaccag tgatgagtct cacgatggtc agccggtggc
     1321 cgccagtttg gtggatgagc tgaaagccgg ccccgtagtg ctgggccggg ctggtgccca
     1381 ggtgttgggt atcgtcaacg atgtcagcca cagtcggcgc acgattaagg cttacttacc
     1441 gggcaagcac tggttggacg ctcagggtcg catcggtttc aatttttccg gcattgtaga
     1501 tccttcttcc aacctgatta agattgatgc agtgccctcg gagaagacca gtgtcaatca
     1561 ggtaaagaag accagagccc agcttgtggc caataaagac ggtgaatatg tagttaagta
     1621 tgctgtaggc aagtatgccg ccatggatct agctatcggt accatcggtc tggccactgg
     1681 accggtgggg atacttacta gc
//
LOCUS       DAOQNZ010001018         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_53388_length_1693_cov_4.717338, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001018 DAOQNZ010000000
VERSION     DAOQNZ010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53388_length_1693_cov_4.717338"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..664
                     /locus_tag="PKW73_15910"
     CDS             107..664
                     /locus_tag="PKW73_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HNA74835.1"
                     /translation="MKKLLLCLTLVLPLTGFVAPAFAGEAEACRKFVQDFYNWYIPPG
                     HKDNDVKDMDQALLKRKNAFSEPLYKALKADSDAASKVKDEIVGLDFDPFLNAQDFAD
                     KYVAKEVKPKGPSYLAAIHSVNGGKVSAKPDLEAELQPSKKGGFVFVNFHYGKSSIPE
                     NENLLSILKVLANDRKKYQSGADKH"
     gene            750..962
                     /locus_tag="PKW73_15915"
     CDS             750..962
                     /locus_tag="PKW73_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74836.1"
                     /translation="MAKVVDNDAPKSLIIEFKTPSGEVWATLTAEGREFKTGSTGFYA
                     NGKVKNPKNGLPYQVGCNIILVGSKE"
     gene            1089..>1693
                     /locus_tag="PKW73_15920"
     CDS             1089..>1693
                     /locus_tag="PKW73_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA74837.1"
                     /translation="MSPRDTEKHVGAFEETGKETASGNAFVDGAKKLWQEAYEHPVAS
                     AAIVAGIAVTTYATRGQVGKILGSGGKSVLLVEDTPYAAQTMKTLLEKEGQSVTWLTG
                     VKRANPYITGITESGQEMVVDPRRYKMAFVDGELKGSYLQGVHVVDALKKEGVPSIGI
                     SSQPTENAALRLNGAIAATEKPTAMTTLASHKFDLGLALRNP"
BASE COUNT          439 a          432 c          437 g          385 t
ORIGIN      
        1 ctggcaggga ccgccgcccg tgctgtcggc cgcaggaagc gccccggatg cttccaactc
       61 tcgttgaaac aaaaccacat tgagactcct gaccatgaag ccttatttga aaaaacttct
      121 tctatgcctg accctggttc tgccgctgac cggctttgtc gcccctgctt ttgccggcga
      181 ggcagaggct tgcagaaagt ttgtgcaaga cttctataac tggtatattc cacccggtca
      241 taaagacaac gacgtgaaag acatggacca ggccttgctc aagcgcaaga acgctttttc
      301 cgagccgctc tataaggcct tgaaagccga cagtgacgct gcttctaagg tgaaagacga
      361 aattgtcggt cttgatttcg accccttttt gaatgcccag gattttgccg ataagtatgt
      421 tgcgaaggaa gtcaaaccaa agggaccctc ttatctggcc gccattcatt ccgtcaatgg
      481 gggcaaggtt agtgccaagc ctgatctgga agctgagctg caaccttcaa agaagggcgg
      541 tttcgttttc gtcaactttc actacggcaa aagcagtatt cccgagaacg aaaacctgct
      601 cagtatattg aaggtattag caaacgatag aaagaagtat cagtcaggcg ctgataagca
      661 ctgataccta tggttgggaa gggcttcttt cgctttatgc aggtgctaaa ctttagacgg
      721 ttttagcact ttacctgcga ggaagcaaaa tggccaaagt tgtagacaac gatgctccca
      781 agtctctcat cattgagttc aagacccctt ccggtgaagt ctgggcaact ctgacagctg
      841 aaggtcgtga attcaaaacc ggctcaacag gattttacgc caacggtaaa gtgaaaaatc
      901 ccaagaacgg actgccttac caggtcggct gcaatatcat tctggtcgga tcgaaggaat
      961 aacttgccgg agacggcaca ttaaaagcga ctgcggcgga ttaatctgtt ctgcgtattt
     1021 ttcccagaga caaagacacc tgaagactgt aactatgtga tcgtatatgt tactgccagg
     1081 taaacgctgt gtccccccga gatacggaaa agcatgttgg cgcttttgaa gagaccggca
     1141 aagaaacggc ctccggcaac gcctttgtgg acggggccaa aaagctctgg caggaagcct
     1201 atgagcatcc agtggcttcc gccgccatcg tggccggtat agccgtgaca acttatgcca
     1261 ccaggggcca ggtcggtaaa attctaggct cgggcggcaa atcggtttta ctggtggaag
     1321 acacgcctta tgccgcccag accatgaaga ccctgctgga aaaggaaggg cagtcggtca
     1381 cctggctcac cggcgttaaa agagccaatc catatatcac cggtatcacc gagtcggggc
     1441 aggagatggt tgtcgaccct cggcgataca aaatggcttt tgtagacggc gagttgaagg
     1501 gcagctattt gcaaggagtt catgtggtag atgccctcaa aaaggagggc gtgccaagta
     1561 tcggcatctc ttcccaaccc accgaaaatg cggcactgcg cctaaatggt gccattgccg
     1621 ccacggaaaa gcccacggcc atgactactc ttgcttctca taagttcgat ctcggtcttg
     1681 ccttacgcaa tcc
//
LOCUS       DAOQNZ010001019         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_53413_length_1693_cov_3.009768, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001019 DAOQNZ010000000
VERSION     DAOQNZ010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53413_length_1693_cov_3.009768"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKW73_15925"
     CDS             complement(<1..626)
                     /locus_tag="PKW73_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74838.1"
                     /translation="MEKRTGALELLMLTGCLAGFTGLEAALAQGGAVSLRQGQTVVTQ
                     DRNLQYGVQCLHSGRFRDAQYYLEIYTKRVPTDWQAQYYLALSREKLGKKEEAGNLYK
                     EIAINCLEKPLGLSAVTALKHIDPDWVTANIAMVKKIGNVTVPVKEKASPLRPTHSSS
                     TAKTPPSQGLPRETRVHFEPGSRGIKLKAEINGRPVDMLFDTGAAGVCL"
     gene            complement(634..>1693)
                     /locus_tag="PKW73_15930"
     CDS             complement(634..>1693)
                     /locus_tag="PKW73_15930"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HNA74839.1"
                     /translation="PADAYAAYYAALAYQQLGNMNSARYYYKIVATVSPTSQIGGYAK
                     TILSKIDPSFVGSASSSASRESTSLGSTRAATVSSTTAIDPRLPKECDVRCRKERGSV
                     VVTPTLNGRPIDMVFDTGAPGIVLGKDQLESLGIKAPSGQPAGVTGGSSNTNEIKYWE
                     MMATVKLGPIEKEMPIEILETNDSQPLFGQTFFKEFDYTIDQGGGSIHFRQKGLGSAG
                     KDRNAYSVPFEFRKAGNRIIVTVEINGRPGKAMFDTGNSANAICFTSDKQARKFGVTI
                     PEDARISYHSGVSGRGQVRIFTVGHVRMGPIDESNVECSVNQDGDDDDLPLLGEPFWK
                     NYQYTIDMNTKQIHFVRR"
BASE COUNT          352 a          447 c          448 g          446 t
ORIGIN      
        1 aggcagactc cggccgcgcc ggtgtcaaag agcatgtcca ccggtcggcc gtttatttcc
       61 gccttcagtt tgatgcccct tgaaccgggc tcgaaatgca cccttgtttc tctaggcaga
      121 ccttgcgagg gcggtgtctt tgcggtcgag gaagagtgag taggtctgag cggggaagct
      181 ttttctttca caggtacggt gacgttgccg atcttcttta ccatggcaat attggccgtt
      241 acccagtcag ggtctatgtg cttgagcgca gttacggcac tcaagccgag cggcttttcc
      301 aggcagttga tggcaatttc cttgtacagg ttgccggctt cttctttctt accaagcttt
      361 tctcggctca gagccagata gtactgggcc tgccagtccg tcggcacacg ctttgtgtaa
      421 atctccaggt agtattgggc atcccggaag cgaccggagt gcaggcactg cactccatac
      481 tgcaaattcc tatcctgggt gactaccgtt tgaccttgcc gcaagctgac ggcgccgccc
      541 tgtgccagag ctgcttcaag accggtaaag cctgccaggc agcctgtgag cataagcagt
      601 tccaacgccc cggttcgctt ttccatttgg tacctagcgt cttacgaagt gaatttgctt
      661 ggtattcatg tcgatggtgt attgatagtt tttccagaac ggctcgccaa gcagcggtaa
      721 atcgtcgtcg tcaccatctt gattgactga acattccaca ttactctcat cgatgggtcc
      781 catgcgcaca tgaccgacgg tgaaaattct gacctgcccc cgccctgaga cccctgagtg
      841 atatgaaatt ctcgcgtctt caggaatggt gactccgaat ttgcgagcct gcttatcgga
      901 agtaaagcaa atggcattgg cactattgcc ggtatcaaac atggctttgc cgggccgtcc
      961 gtttatttca accgtgacga taattctatt gccggccttg cgaaactcaa aaggcacgct
     1021 gtaggcattt ctgtctttcc cggcgctgcc cagacctttc tgccggaagt gaatactgcc
     1081 tccaccctgg tcaatggtgt aatcaaattc cttgaagaat gtttgtccga aaaggggctg
     1141 agaatcgttt gtttcgagaa tctcaatggg catttctttc tcgatggggc caagtttaac
     1201 tgtggccatc atttcccagt acttaatttc attggtgttg gaggaaccac cggtaactcc
     1261 agccggttgt ccactgggag ccttaatgcc gagactttcc agttggtctt tgccaagtac
     1321 aattcccgga gcgcctgtgt caaagaccat gtctatgggc ctgccattca aagtcggtgt
     1381 caccaccacc gaaccacgct ccttgcgaca gcgcacatcg cattctttag gcaaacgtgg
     1441 atcgatagcc gtagtgctgg agactgtagc ggctctggta ctgccgagac tggtgctttc
     1501 ccgtgaagcg gaagaggagg cgctgccgac gaaactggga tcgattttgg aaagtattgt
     1561 cttggcatag ccgccgatct ggcttgtggg gctgactgtg gccactatct tgtaatagta
     1621 cctggccgaa ttcatattac ccagttgctg ataggcaagg gctgcatagt aagcagcgta
     1681 ggcatccgcc gga
//
LOCUS       DAOQNZ010001020         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_53513_length_1692_cov_2.246793, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001020 DAOQNZ010000000
VERSION     DAOQNZ010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53513_length_1692_cov_2.246793"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKW73_15935"
     CDS             <1..461
                     /locus_tag="PKW73_15935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74840.1"
                     /translation="GAINGRLYRLRAECLESLGQYREAVSDYKQAMKDPRTGGTAAIF
                     MARCYERLGDGKSALKAAEEAIAREPGNRLYQMEYGRLLSKAGRYEDAIKQYDHCLTI
                     RRDWPTPYFERAKCLRQVGRLSEAAADEARGRALAAPIADDLGVHVRTTK"
     gene            505..>1692
                     /locus_tag="PKW73_15940"
     CDS             505..>1692
                     /locus_tag="PKW73_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74841.1"
                     /translation="MSSEDSTSEFEQESGHAVWKYLQEVNRFSRGEPVFGLPEVTPRA
                     LKVGLLARSRLLARQSLENIARRCHLSDLYLVTAACLDENASFRELAWEVVSKILSTL
                     GMEQLACVDSSMRSYYLEVPGEFGDLTPPPAVLGSLSFNRSGHLREQAVQRLAGVWTG
                     EELPFLLLRGRDWVPEVASIAAEALRARAGKVEYGKLFAHNLGLIAVLSRSGQPRVRE
                     LAALSERHSLVGEGRAVLADYLRGKDKELRCYAADICEKYDQALLVEVGLNSSDPRLR
                     LKAFMAMLSLDSGLEASASGSGSGPGSGSGSGLDADFRNVSPISYRAFLSDPDPAIRV
                     EALRKLQEVESAEQLRDAAIEALLDRSGRVRLLARYILKDIDPFVLYLDRLASTRVTD
                     SVLP"
BASE COUNT          329 a          400 c          558 g          405 t
ORIGIN      
        1 agggtgccat aaacgggcgt ttgtaccgtt tgcgggcgga gtgtctggag tctctggggc
       61 agtatcggga ggccgtttcc gattacaagc aagccatgaa agatcccaga acgggcggca
      121 ccgctgccat ttttatggct cgctgctatg agaggctggg agacggaaag agcgctctta
      181 aagcggcgga ggaagccatt gccagggagc cgggtaatcg tctctatcag atggagtatg
      241 ggcgattgct cagtaaagcg ggccggtatg aagacgccat caagcagtat gaccattgtc
      301 tgaccattcg ccgagattgg cccaccccct attttgagag ggccaagtgt ttgcggcagg
      361 tggggcgctt gagtgaggcg gctgccgatg aagcgcgggg gcgcgctctc gctgctccca
      421 tcgccgatga tctgggggtt catgtgagaa cgacaaagtg agggcaggcg tttttgcaat
      481 tctcagggtg gtagacttgg ggttgtgagc agtgaagatt ctacatctga atttgagcaa
      541 gaatccggcc atgcggtctg gaagtatttg caggaagtca accggttttc ccggggcgag
      601 cctgttttcg gcttaccgga agtaactccc cgggccctga aggtgggtct gctggcgcgt
      661 tcacgcttgc ttgccaggca gtccttagag aatattgcca ggcgttgtca cctcagtgat
      721 ttgtatttgg ttacagccgc atgtctggat gaaaatgcct cattcaggga gcttgcctgg
      781 gaggtggtaa gcaagattct gtccactttg gggatggagc agcttgcttg tgtggatagt
      841 agtatgcgct cctactacct ggaagttccc ggagagttcg gcgatctgac gccgccgccg
      901 gcagtactgg gtagtctttc cttcaatcgc agcggccact tgcgagaaca agccgtgcag
      961 cggctggccg gcgtgtggac aggagaggag ttgccctttc tattgctgcg ggggcgagat
     1021 tgggtgccgg aagtagcgag cattgccgcc gaggcgctaa gggcgagggc tgggaaagta
     1081 gagtacggta agctttttgc ccataatctg gggctgattg cagtcttgag ccgttccggg
     1141 cagccccggg tgagggaact ggctgcgctt tcggagcggc atagccttgt tggtgagggg
     1201 cgggccgttt tagccgacta tctaagaggc aaagacaaag agctgcgctg ctacgccgca
     1261 gatatctgtg agaaatacga ccaggccttg ctggtggaag tggggctaaa ttcttcggat
     1321 cctcgcctga ggctgaaggc tttcatggct atgctttctc tggattccgg gttggaggct
     1381 tcggcttcag gttcaggctc cggccccggt tcaggctcag gctcaggttt ggacgcggat
     1441 tttagaaatg tgtccccaat ttcttatcgg gcttttctaa gcgaccccga cccggctatc
     1501 agagtggagg cactgcgaaa actacaggaa gtggagagtg ccgagcagct aagagatgcg
     1561 gctatagaag ctcttctcga tcgctccggt cgggtgcggc ttctggctcg ctatattttg
     1621 aaagacattg atccttttgt tttgtacctg gatcgcctgg cgagcaccag ggttaccgat
     1681 tctgtcttgc cc
//
LOCUS       DAOQNZ010001021         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_53619_length_1690_cov_3.300306, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001021 DAOQNZ010000000
VERSION     DAOQNZ010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53619_length_1690_cov_3.300306"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKW73_15945"
     CDS             <1..285
                     /locus_tag="PKW73_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74842.1"
                     /translation="QERAEFKDRETSTYFLGWDEHKLPWLELFEKLKADGIFRDMSSE
                     AVYEFMSNQVYGTLFANYFSGKKKPLASQTGVTLDILYRGILQGGQRSEF"
     gene            329..>1690
                     /locus_tag="PKW73_15950"
     CDS             329..>1690
                     /locus_tag="PKW73_15950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA74843.1"
                     /translation="MIRKTLMEVLNDKTEPPAKPEQPFRQPGPASASGLSRGKMILAL
                     MALLGIGLGLYFGLGGASKKEVAAVSDKAVLSVRLSPVTSRSMERKLLVNGTVWSWDH
                     LSIGSEVGGLKVDKVLVDEGDQVREGQVLATLQSSILKAQLNREEARLLAAGAALSKS
                     IQPNRPEDLTALRAAYAQTEASVAQEEAGLVRARANYQEASENAKRYRWLVAQGAVSA
                     QEADARETQAKVAMADVNNYVERVKAAKFSSQQAHERLSMGLTGGRREDVDIARAQKM
                     EIEAVIKQLRWQIEQTVVRAPCSGVIMKRHVHLGDIASNTKTMFEIVRDGRLELKAQV
                     PEQDLSQVKPGQTVEMTDDFGGSFRGQVREISPQVDQDMRLGTVRIDITNPGSLKPGN
                     FLRGEILVGKAQVLVVPTSCVIYKGNRPLVYLVEDGKVKMSYVDVGGREGKFVEIVSG
                     LKLG"
BASE COUNT          374 a          402 c          539 g          375 t
ORIGIN      
        1 caggagcggg ctgagttcaa agaccgggag acttccactt attttctcgg ttgggatgaa
       61 cacaaattgc cctggctgga acttttcgag aagctcaagg cggacgggat cttccgagat
      121 atgtcctcgg aggcggtcta tgagttcatg agcaatcagg tttatggcac tttgttcgcc
      181 aattattttt caggcaagaa gaagcctctg gccagtcaaa ccggcgttac cctcgatatt
      241 ctctaccggg gaattttgca gggggggcaa aggagcgagt tttagaaatg gctttgcagc
      301 cgttcctggc tgaccggacc tggggatcat gataaggaag accttgatgg aagtgctcaa
      361 cgacaagacc gaaccaccag ccaaaccgga gcagccgttc cggcagccag ggcctgcttc
      421 tgccagcggt ctcagccgtg gcaagatgat cctggcgcta atggctttgc ttggtatcgg
      481 gttgggtctt tactttggtc tgggcggcgc ctccaagaaa gaggttgctg cggtgtccga
      541 caaggcggtg ctgagcgtgc gcctttcccc tgttaccagt cgttccatgg aacggaaact
      601 tttggtgaac ggcacggtct ggtcctggga ccatctttcc atcggctcgg aagtgggcgg
      661 tttgaaagta gacaaagtgc tggtagatga gggtgatcag gtgcgggaag ggcaggttct
      721 ggctactttg cagtcttcca tcttgaaggc ccagttgaac cgtgaggagg cgaggctttt
      781 agcggccggg gcggctcttt ctaaatcgat tcagcccaac cgcccggaag atctgacggc
      841 tttgagagcc gcctatgctc agaccgaggc ttcggttgct caagaagaag caggtctggt
      901 gcgggcccgg gccaactacc aggaagcttc tgagaacgcc aaacgttacc gctggctggt
      961 ggctcagggg gcggtcagcg cccaggaagc cgatgcccga gagacccagg ccaaggtggc
     1021 catggccgat gtgaataact atgtggaaag ggtcaaggct gctaagttta gctcccagca
     1081 agcccatgag agattgtcca tggggctgac cggcggacgg cgtgaagatg tggacattgc
     1141 ccgggcgcag aagatggaaa tcgaagcggt gatcaagcaa ttgcgctggc agattgagca
     1201 gactgtggtg cgtgcgcctt gttccggtgt aatcatgaaa agacatgtgc accttggtga
     1261 cattgcttcc aataccaaaa ccatgtttga aattgtgcgc gatggacgtc tggagctgaa
     1321 ggcccaggtg cccgaacagg atctcagcca ggttaagccc gggcagaccg tggagatgac
     1381 cgacgacttc ggtggtagtt tcagagggca ggtgcgcgag ataagccctc aggtagacca
     1441 ggacatgcgc ctgggtacgg tgcgcatcga tatcaccaat cccggcagtc tcaaaccggg
     1501 taatttctta cgtggtgaaa ttctggtggg caaagcccag gtgctggtgg tgcctacttc
     1561 ctgtgtcatt tataagggta atcggcctct ggtctactta gtggaagacg gtaaggtgaa
     1621 gatgtcttac gtcgatgtag gcggacgcga gggcaaattc gtggaaattg tctccggtct
     1681 aaaactgggt
//
LOCUS       DAOQNZ010001022         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_53690_length_1689_cov_3.238066, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001022 DAOQNZ010000000
VERSION     DAOQNZ010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53690_length_1689_cov_3.238066"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKW73_15955"
     CDS             <1..298
                     /locus_tag="PKW73_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74844.1"
                     /translation="QSFGGLVGAPVDPRYGQSNEVGMLADYGYDTARDISRALTLIAL
                     LISTAVAIGFLRGRRPMTFGAVTKAIGIALVAPLAVHVVGTFLINNFGGLGGGL"
     gene            complement(380..769)
                     /locus_tag="PKW73_15960"
     CDS             complement(380..769)
                     /locus_tag="PKW73_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74845.1"
                     /translation="MGSAFKLTGSYILAGNLIEPGEKLNALANHNNPAVRRRLAENPG
                     TPAETLAQLASDENHEVRAALSWNAAVQMELLARLCKDEDVNVRLALTDNTSLPSELL
                     ELLAADENPYVQYHARRALELRGTEPR"
     gene            complement(1002..>1689)
                     /locus_tag="PKW73_15965"
     CDS             complement(1002..>1689)
                     /locus_tag="PKW73_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74846.1"
                     /translation="GHEDQARMLDTFASAFLGVNGGTAVGPKYSIASAVLAKEEPSGF
                     WATVGETMVTRSKQICRSIKDRTYIKPGDAFINTQFFTETSIAKGRLKLPCVCQEDAA
                     SWQKMPGLWLTNTGTTVRITCSSNSELTGTIMSVPADSPFQVKPGDKTFQAARPTANN
                     AVHSDNGFCYPKKGDKLTPRANCPVDLTISADGKTITGTRTLLQYYDGSTTWDAGSIV
                     SQLSWKRIGD"
BASE COUNT          343 a          426 c          467 g          453 t
ORIGIN      
        1 gcaatctttc ggcgggctgg tgggagcacc ggtagatccc cgctacgggc aatcaaatga
       61 agttggaatg ctggccgatt acggttacga tacggccagg gatatttccc gagcgctgac
      121 ccttatcgct ctgctcatat ccacagcagt ggctataggc tttttgcgag gccggcgacc
      181 gatgaccttc ggtgccgtaa ccaaggccat cggcattgct ttggttgcac cgctggcggt
      241 tcatgtggtc ggcaccttcc tgatcaataa cttcggcggt cttggcgggg gcctatgagg
      301 caagagtcga ctacttgaga aataagggtg gagcctcgat ttatattgag gctcccctct
      361 gtttggattg cggccgttgc tatcgtggtt ccgtacctct aagctctagg gctcttctgg
      421 cgtgatattg aacatatgga ttttcatcgg ctgcaagcaa ctcaagtagt tctgacggca
      481 gactggtgtt atccgtcagc gccaggcgca cattgacgtc ttcgtcttta cacaggcggg
      541 caagaagttc catttgtacg gcggcgttcc aggagagcgc cgctctcacc tcatggttct
      601 catcgctcgc caattgtgcc agcgtctcag ccggcgtgcc aggattttct gccaatctgc
      661 gacgaacggc agggttatta tgattagcca gtgcatttag cttttctccc ggctcaatta
      721 ggtttcctgc cagaatgtat gatcctgtga gtttgaatgc gctacccatc ccgcccctcc
      781 tgaattcgta gaagcaaaca gtgtcttcaa ttgtgaaaag agatcgtgtg cggtctgcga
      841 ctttaagtac ctgatagtat ccagcattac acgagctaag caatcgttac tatcccccga
      901 ctggggaact aacgattgct tagcgttatc ctgtgcggat caggcgtcct ttagcaatcc
      961 tatttgatgc ttcgcttgct agaagcaaag cgctttctat tctagtcgcc gattcgcttc
     1021 catgagagtt gagagacaat cgaaccggca tcccaggttg tggagccgtc atagtattga
     1081 agtaaagtgc gcgtgccggt tattgtttta ccatcggcag atatggtcag atctactggg
     1141 cagttggccc ttggcgtcaa tttgtctccc ttcttcggat agcaaaagcc gttgtcgcta
     1201 tgtactgcgt tattggcagt ggggcgagcc gcttgaaacg ttttgtcccc cggctttact
     1261 tggaagggcg agtcggctgg aacgctcatg attgtcccgg ttagttccga attagatgaa
     1321 caagtgattc taaccgtggt gccggtgttg gtcagccaca agcccggcat tttctgccag
     1381 gatgccgcat cttcctggca gacgcagggt agtttgagcc tgcctttggc aattgaagtt
     1441 tctgtgaaaa actgggtgtt tataaaggca tcgcctggtt tgatgtaggt cctatccttt
     1501 atggagcggc aaatttgttt ggagcgcgtt accatggttt cgcccacggt agcccagaaa
     1561 cctgagggtt cctctttggc cagcaccgca gaggcgatgg agtacttggg accaacggcg
     1621 gtgccgccgt tcactccaag aaatgccgag gcaaaggtat cgagcatacg ggcctggtcc
     1681 tcatgcccg
//
LOCUS       DAOQNZ010001023         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_53910_length_1686_cov_4.267321, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001023 DAOQNZ010000000
VERSION     DAOQNZ010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53910_length_1686_cov_4.267321"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..671
                     /locus_tag="PKW73_15970"
     CDS             129..671
                     /locus_tag="PKW73_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74847.1"
                     /translation="MVSAAYVNNTARVIWQNYYDVATVAASSGNWGTASLMYRESLST
                     ATEAGLTEEEAFTCHGLALVLLQTRQSEEAERFLRRAIRLYSLLTPLAARGLASVVSV
                     LADLYTARNADERALPLLKMCERSITTVYGVQAPELLPIVSRMALIYNRHSQHGKAEE
                     CVKHYRSLKEKLLFNPASQA"
     gene            complement(649..>1686)
                     /locus_tag="PKW73_15975"
     CDS             complement(649..>1686)
                     /locus_tag="PKW73_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA74848.1"
                     /translation="FMIIVTMAVIGPALIWFLGGKLVIDGALGLGTVTAFVALLSRLY
                     TPVTSLSGVQVQIASALAVFERIFEYLDLPEEEQCGEDLPQSVKGELEFDQVSFSYNS
                     ERPTLEDISFRIEPSKVLAVVGHSGAGKTSLANLIPRFHEIAAGEIKLDGKNIKTYKL
                     SSLRAHIGMVTQETYLFHDTIEANLRYAKPQAGREEIEEAARLAHIHDLVMSLPDGYQ
                     TTVGERGYKLSGGERQRLSIARVILKNPAILILDEATSALDSVSEEAIQKALKELTRG
                     RTSLVIAHRLSTVEDADKIIVLDHGKIVESGTHRELMLARGKYAELAARQFKGRNQQE
                     EEEPLKLEKRD"
BASE COUNT          342 a          462 c          470 g          412 t
ORIGIN      
        1 gctgccttcc gtctctttct tccggcccgg taaagtggcg accattttgc aagactgtgt
       61 gtttgcacag agctttgccg gcttttgcat tttaagcttg cctgcatgag cagtcgggag
      121 tgaatgtcat ggtcagcgca gcatacgtaa acaatacggc cagggtgatc tggcaaaact
      181 attatgatgt ggcaacggta gctgcctcgt cgggtaactg ggggacggct tccctcatgt
      241 accgggaatc tctctccact gccaccgagg ccggcttaac ggaggaagaa gcttttacct
      301 gtcacggttt agccctggta ttgttgcaga cccggcagag cgaagaagcc gaacgttttt
      361 tgcggcgggc cattcggctc tactctctac ttacgcctct tgccgcaagg gggcttgctt
      421 ctgtggtgtc ggtgctggcc gacctttaca cagcccgcaa tgccgatgag agagccttgc
      481 cccttttgaa gatgtgcgag cgctccatca ccacggttta cggcgtgcag gcgccggaac
      541 ttttgcccat cgtcagccgt atggccctta tctacaaccg gcatagccag cacggtaagg
      601 ccgaggaatg cgtcaagcat tatcgaagct tgaaagaaaa gcttctgttc aatcccgctt
      661 ctcaagcttg aggggctctt cttcttcttg ctggtttcgc cccttgaact gcctggcggc
      721 aagctcagca tatttgcccc gggccagcat aagctcacga tgcgtaccgc tttctacaat
      781 tttaccgtgg tcgagcacga taattttgtc ggcatcttcc acggtcgata gccggtgcgc
      841 gatgaccaga ctggttctgc cccgtgtcaa ctctttcaag gctttttgaa tggcttcttc
      901 gctgacgctg tcgagggcgc tggtggcctc gtcgagaatc agtatggccg ggttcttcaa
      961 aatcactcgg gcgatggaga gtctttgcct ttcgccgccg ctcagcttgt agccgcgctc
     1021 gcccaccgtc gtttgatagc cgtcaggcaa agacatgacc agatcatgga tatgagccag
     1081 acgcgctgct tcttctattt cttcccggcc ggcctgaggt ttggcgtaac gcaaattggc
     1141 ttcgatggtg tcgtgaaaga gataagtttc ctgggtgacc ataccgatat gcgcacgcag
     1201 ggaggagagt ttgtaggtct tgatgttctt gccgtccagc ttgatttcgc ctgccgcaat
     1261 ctcgtggaag cgaggaatga ggttggccag cgaggtcttg cctgcgccgg aatgaccgac
     1321 cacggccagc acttttgagg gctcgatacg aaaggatata tcctccaggg tgggtctttc
     1381 gctgttgtat gagaagctca cctggtcgaa ttccaactca ccttttaccg actgaggcag
     1441 gtcttcgccg cactgctctt cttcgggcag atccaggtat tcgaagatgc gctcaaacac
     1501 agcaagggcg gaggcaatct gcacctgcac ccccgataga gaagtgaccg gtgtataaag
     1561 cctggaaaga agtgccacaa aagccgtgac cgtgcccaga cccagggctc cgtcgatgac
     1621 tagtttgccg cccaggaacc agatcaaagc cggacctatg acggccatgg tgacgatgat
     1681 catgaa
//
LOCUS       DAOQNZ010001024         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_53922_length_1686_cov_3.586757, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001024 DAOQNZ010000000
VERSION     DAOQNZ010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53922_length_1686_cov_3.586757"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKW73_15980"
     CDS             <1..348
                     /locus_tag="PKW73_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74849.1"
                     /translation="YVFKPTFFMILFIVYEGVMVWLLSHSNPGLRIAGIVPGVAALLS
                     GYVLVSCLVWSATGSLKDGPMRLMAVKMYNDILKLDVGHDMAIKGTSMALGLPPERVK
                     EWILAQKGKMRIS"
     gene            439..>1686
                     /locus_tag="PKW73_15985"
     CDS             439..>1686
                     /locus_tag="PKW73_15985"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNA74850.1"
                     /translation="MSSDSKDNFGQGKSGQSGNMPASVPAAADLADSGFAEAPFRFPW
                     LRLLQVVLDAGLAAASLAGAYFIRFEGHLSPLYLQQFLVVAPVVVALRLVANWRCGVY
                     GRLWRYTGLTEVTEIGLAVLTVSGLLLCLRAMGLPLMKVEGHLLSFSILVIDMGICYF
                     LMTASRVLRRLQTEHSQRRHWRQPVRRRALVVGAGDAGLMVLKELTQRSDLGVDVVGL
                     VDDDPSKLKKRIGHITVFGATRELPRLVELLCIDQVIIAMPSAPAAEIRRIVDMCREA
                     EVETRILPGLFELINGRVSINQLREVSLEDLLGRSPVELDSASIASYIEGRTVLVTGA
                     GGSIGSELCRQIFHFQPRKLIILGKGENSIFTIQQELSRRVEPVEVVAVIADVRDRPR
                     MEQIFSEHKPHLVFHAAAHKHVPL"
BASE COUNT          305 a          448 c          487 g          446 t
ORIGIN      
        1 tatgtcttca agcctacttt cttcatgatt ctcttcatcg tttatgaagg cgttatggtt
       61 tggctgctca gtcatagcaa ccctggtctg cgtattgccg gcatagtgcc cggtgtggcg
      121 gctctgctct ccggttatgt tctggtttcc tgcctggtct ggagtgccac cggttccctc
      181 aaggacggac cgatgcgtct catggctgtg aagatgtaca acgacattct caagctcgat
      241 gtcggccacg atatggccat caagggcacc agtatggcac tgggcttgcc tccggagcgc
      301 gtgaaagagt ggattcttgc ccagaaaggt aagatgcgta tttcctgatt gctcaggtga
      361 gtttcgcgta agcaaagcac ctcctgacta gtgctaatat agcctcctga gttaaggaag
      421 caatcttagc gggcgtttat gtcttctgat agcaaggaca attttgggca aggcaagtcc
      481 ggccagtccg gtaacatgcc ggcttcagtg ccggcagcag cagatcttgc cgattccggt
      541 ttcgccgagg ctcccttccg tttcccctgg ttgcgtctcc tgcaggtagt gctggacgcc
      601 gggcttgctg ctgcttcgct ggctggtgcc tatttcattc gttttgaagg ccatctctct
      661 cccctttact tgcagcagtt cctggttgtt gcaccggtgg tggtggcgct tcgcctggtc
      721 gccaactggc gctgcggcgt ttatggacgt ctctggcgtt acaccggtct gacggaagta
      781 actgaaatcg gacttgccgt tttgactgtt tccggtttac tgctatgcct cagggctatg
      841 ggtttaccgc tcatgaaagt ggaagggcat ctgctctctt tcagcattct ggtcatagac
      901 atgggcattt gctatttcct tatgaccgcc agccgagtct tgcggcgctt gcaaacagag
      961 cacagtcaga ggcggcactg gcggcagccg gtgcgccggc gcgctctggt ggtgggggcc
     1021 ggcgacgccg gtttgatggt gctgaaagag ctgacccagc gcagtgacct gggcgtggat
     1081 gtggtcggtc ttgtagatga cgatccgagc aagctgaaga agcgtatcgg gcatatcacc
     1141 gtatttggtg ccacgcggga actgcccaga ctggtggaat tgctttgcat cgatcaggtg
     1201 atcatcgcca tgccttccgc cccggctgct gagattcgcc gtatcgttga tatgtgtcgt
     1261 gaggctgagg tcgaaacgcg aattctgccc ggtctcttcg agcttatcaa cggacgagtc
     1321 agcatcaatc aattgaggga agtctcactg gaagatcttc tcggccgctc cccagtagaa
     1381 ctggactctg cctccattgc ttcttatatc gaagggcgta ccgtgcttgt taccggtgcc
     1441 ggtggttcca tcggttcgga actgtgccga cagatctttc atttccagcc gcgcaagtta
     1501 atcattctgg gtaagggtga gaattctatt ttcaccatcc agcaggaact ttcgcgccga
     1561 gtcgagcctg tagaggtagt ggcagtcata gccgatgtgc gcgaccgtcc gcgcatggag
     1621 cagattttta gcgagcacaa gccccatctg gtcttccatg ctgccgccca caagcatgtg
     1681 cccttg
//
LOCUS       DAOQNZ010001025         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_53978_length_1685_cov_4.273620, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001025 DAOQNZ010000000
VERSION     DAOQNZ010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53978_length_1685_cov_4.273620"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKW73_15990"
     CDS             <1..775
                     /locus_tag="PKW73_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNA74851.1"
                     /translation="QTFALSCRNSVGMLKLNRPAQVNCLPMAFFEELPGAIRQLEELD
                     CRALIIHGQGKHFCAGIDLSVLGDEGFLKASTLKEKERLQALILRLQAALTSLSEAKF
                     PVLAAVDGYCLGAGLDLVSACDFRFATEKAQFAIEEINVGLMADLGTLQRLPRLMPEG
                     LVRKMAFTGERLSGKRAFDCGFVSELFPDAESMLKAAEECAARMAGKESSAMAASKQA
                     LNFNGGHTVSDSLQECARLQALYLRPEVVMENVKRIKMR"
     gene            858..1625
                     /locus_tag="PKW73_15995"
     CDS             858..1625
                     /locus_tag="PKW73_15995"
                     /inference="COORDINATES: protein motif:HMM:NF021371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HNA74852.1"
                     /translation="MSLLFAKFAATLLETAHRPFPLAAGPWVMNQTWSNLLFAHWCVP
                     LESMRKILPPCFEMDLFEGRAYIGVVPFLMEDVSPRYCPNMPWLSTFPELNVRTYVRY
                     KDQPGVYFFSLDAGNPVAVWVARTAYHLPYFNAEMRIEQGADGYFHYQSLRKEASEKI
                     AAPCLRARYKAVGPVWKSVPGSLDHFLTERYCLFTTHGSRVYRGDIHHKPWPLQDAVA
                     DFQVNTMTAPLGLELSGEPLLHFVEGIETVEWPIQAC"
BASE COUNT          332 a          446 c          482 g          425 t
ORIGIN      
        1 ccagaccttc gctttgagct gccgcaactc cgtgggcatg ctcaagttga atcgtcccgc
       61 ccaggtaaac tgtctgccta tggcattctt cgaagaattg cccggggcga ttcggcaact
      121 ggaagaactg gattgcaggg ctcttatcat ccacggccag ggcaaacatt tttgtgccgg
      181 tatcgatctt tcagtgctgg gcgatgaggg ctttctcaag gcttccacct tgaaagagaa
      241 agagcgcttg caggctctga ttttgcgctt acaagccgct ctcaccagtc tgagcgaggc
      301 taagtttccg gttctggctg ccgtagatgg ttactgtctg ggtgccggtt tggaccttgt
      361 cagtgcctgt gacttccgct tcgctaccga gaaagcgcag tttgccatcg aggagataaa
      421 tgtgggattg atggcagatc ttggcacttt gcagagattg cctcgcctca tgccggaagg
      481 gcttgtgcgc aaaatggctt tcaccggtga gcgcctttca ggcaaacggg ctttcgactg
      541 tggttttgtc agtgagcttt tccctgatgc agagtcaatg ttgaaagcgg ccgaagaatg
      601 tgcggcccgc atggccggta aggaaagctc ggccatggcg gccagcaagc aggctctcaa
      661 tttcaacggc ggtcataccg tcagtgatag tttgcaggaa tgtgcccgtt tgcaagctct
      721 ctatcttcga ccggaggtgg tcatggagaa tgtcaaacgc atcaaaatgc gctgaaattc
      781 tggggtctgc cgcatatttt gccgctatca ttttatcgtc ggcataggtc tgggcgacat
      841 gggccgggag ggattagatg agcttgttgt ttgccaagtt cgccgccacg cttttagaaa
      901 ccgcacacag gccctttccg cttgcagccg ggccctgggt catgaaccag acctggtcta
      961 atttgctctt tgcgcactgg tgcgtgcctt tagagagcat gcgaaaaatt ttgccgccct
     1021 gctttgaaat ggaccttttt gaaggacggg cttatatagg cgtggtgccc ttcctgatgg
     1081 aggatgtcag cccgcgctac tgcccgaata tgccctggct ttccaccttc ccggaactca
     1141 atgtgcgtac ctatgtgcgc tataaagatc agcccggagt gtacttcttc agcctggatg
     1201 ccggcaatcc ggtggcggtt tgggtggcgc gcacggctta tcatttgcct tatttcaatg
     1261 ctgaaatgcg catcgagcaa ggcgcggacg gctattttca ttaccagtcg cttcgcaagg
     1321 aggcttccga aaagattgcc gctccctgcc tgagggctcg ctacaaagct gtgggaccgg
     1381 tctggaaaag tgtacccggc agtctcgatc acttcctgac ggaacgctat tgtcttttta
     1441 ctacccacgg ctcccgggtt tatcgcggcg acatccacca taagccctgg cccctgcaag
     1501 atgccgtggc cgacttccag gtgaacacca tgaccgcgcc cctggggcta gaactttccg
     1561 gtgagccgct tttgcacttt gtggaaggca tagagaccgt ggaatggccc atccaggctt
     1621 gctagtagcc cggggctatt tgagtaactc gctcatggtg cggaagacag ttgctgtctg
     1681 accgg
//
LOCUS       DAOQNZ010001026         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_53992_length_1685_cov_3.278528, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001026 DAOQNZ010000000
VERSION     DAOQNZ010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53992_length_1685_cov_3.278528"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..945)
                     /locus_tag="PKW73_16000"
     CDS             complement(262..945)
                     /locus_tag="PKW73_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA74853.1"
                     /translation="MPKILVVEDDEDLADMVSSWLESERHTVEVANDGTLARDLLKLS
                     SFDVIVLDWDLPGMSGIEVLKEFRGQGGNTPIIMLTGKSEISDKEAGLGTGADDYLTK
                     PFAVRELGARIRALLRRPGNLQSSVLRAGNLELDSAKHRVVVAAQEVHLLPRDFALLE
                     FFLRHQDEVFSVEALLTRVWSSDSDATPEGLRTAIKRIRKKIDGDGGDESKSLIETIP
                     RIGYRLRSR"
     gene            1112..>1685
                     /locus_tag="PKW73_16005"
     CDS             1112..>1685
                     /locus_tag="PKW73_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74854.1"
                     /translation="MLSLNSLRARILILVSVPLVFQLVLLGAVAYLQNEAEDEARRAE
                     ISRKISDEVIELTRDIFAVKSNFGFPDSVHLEPSLGSQYQTLDESIKAHFAKLRELTK
                     GDLELSRALNNVIASMYRTQGEFLNARRTYNRARRLSGMNVNDETVAAYKRLRDLSIE
                     LSTDVVKLGDAGKKIADASPEHQRKFREQVQ"
BASE COUNT          396 a          429 c          445 g          415 t
ORIGIN      
        1 catcaaagac ctgcccatca agcctggtaa gcctggcctg cttccccctg gtaccgaggt
       61 caatatcgac ggtgacgtga atatctacat gggatgtccg gctcctgtcc gcgtcaagcc
      121 gttaggactg aagtaaccga tcttaagttt gatccaaagg atcctgctgt cttttcagca
      181 aggatgagca acagggctct cgtcaactaa atccggacca aatttcgtac gtggaccttg
      241 gcacgtactc tggtcttctc cttaccggga acgcagccgg tagccgattc tggggatggt
      301 ttcaatgaga gatttggatt cgtctccccc gtcgccgtca attttcttgc gaatacgttt
      361 gatggccgtg cgcagcccct cgggggtggc gtccgaatcg gaggaccaga ctctggtcaa
      421 caaggcttcc acgctgaaga cttcgtcttg atggcggagg aaaaactcca gcagagcaaa
      481 gtcgcggggc agcaaatgca cttcttgagc ggccaccact accctgtgtt tggcggagtc
      541 cagctccaga tttccggctc gcaagacgga gctttgcaga ttgccgggac ggcgcaaaag
      601 ggctctgata cgggctccca gttcacgaac cgcgaagggc ttggtgagat aatcatcggc
      661 tccggtacca agaccggctt ctttgtcgga aatttccgat ttgcccgtga gcattatgat
      721 gggagtgttg cctccctgcc cgcggaattc tttcagaact tcaataccgc tcattcccgg
      781 cagatcccag tcaagcacga tgacgtcaaa gctcgatagt ttgagaaggt ctctggcgag
      841 ggtgccgtca tttgctactt cgacggtgtg ccgttcgctt tccagccaac tggagaccat
      901 atcggcaagg tcttcgtcgt cctccaccac aagaatcttg ggcatggtct ttgttttctg
      961 ctccgctttg tctgataaag aatttgagta ggagcagtct gatatttatt gccgccgaac
     1021 tacccagaga ctttttaccc cgggccgacc atgattaact ggaagactta agtcttgatt
     1081 tgatcgcaag agggccggga ataatcagga aatgctaagt ctcaacagtc taagagcaag
     1141 gattctcatt ctagtttcgg tgccgctagt gtttcagttg gtgctgctcg gggcggttgc
     1201 ttatctgcaa aatgaggcgg aagacgaagc ccggcgggcg gaaatttcca gaaaaatatc
     1261 agatgaagta attgagctga ccagagatat ttttgcagtc aaaagcaatt ttggttttcc
     1321 cgattcggtg cacctggagc cttctctcgg ctctcagtat cagactctgg atgagagcat
     1381 taaggctcat tttgcaaagc ttagagagtt gacgaaagga gacctggaac tttctcgggc
     1441 tctcaataac gtcatcgctt ccatgtatcg aacccagggt gaatttctta atgccaggcg
     1501 cacctataat cgagcgcgca ggctctccgg catgaacgtc aacgacgaaa ccgttgctgc
     1561 ctacaaaagg ttgcgcgatc tttcgattga gctttctaca gatgttgtca aactgggtga
     1621 tgccggcaag aaaatcgccg atgccagccc cgagcatcag cgaaaattca gagaacaagt
     1681 gcaga
//
LOCUS       DAOQNZ010001027         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_54042_length_1684_cov_3.979742, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001027 DAOQNZ010000000
VERSION     DAOQNZ010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54042_length_1684_cov_3.979742"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKW73_16010"
     CDS             <1..338
                     /locus_tag="PKW73_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNA74855.1"
                     /translation="GEAEKTLRVLQAEGQRDSDKLKGEGIALQRAEIVKGLTNSIEEL
                     KKSYPGASEEAIMNMLMMNQWLEALTAIGAKSGATTIFVPASPQGMTDFRQQIMEGLV
                     GSVKGAAKS"
     gene            468..611
                     /locus_tag="PKW73_16015"
     CDS             468..611
                     /locus_tag="PKW73_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74856.1"
                     /translation="MEIPNFIAIPALLLFAAAVLFITVRCIKQGKVGPEYGLKIRIKK
                     KKD"
     gene            complement(706..1290)
                     /locus_tag="PKW73_16020"
     CDS             complement(706..1290)
                     /locus_tag="PKW73_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA74857.1"
                     /translation="MDRHENNALIAMTLESFDEVPRFCLPGGFTVRTYRSGDERHWLD
                     LQKKADHYNEFDNSTFEMQFGEGIDELSERQLYIEKNGQIIGTATAWSDNKYWEAQSG
                     RIRWVAVLPEYQGMGIGRALISSVLERFALLGETRSYLWTRTVRLPAIKLYLRFGFKP
                     GIRSKEEEEIWARLIKDNNLQSNYSSRSFFEAGK"
     gene            complement(1499..>1684)
                     /locus_tag="PKW73_16025"
     CDS             complement(1499..>1684)
                     /locus_tag="PKW73_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74858.1"
                     /translation="AVHRFRHWARALALALLPLGAYTGGSYGALAALLLALGVFLFSL
                     AFVGLKGVESDSGSAFD"
BASE COUNT          426 a          476 c          418 g          364 t
ORIGIN      
        1 agggcgaagc cgagaagacc ctgcgcgtcc tgcaggccga aggtcagcgc gactccgaca
       61 agctgaaggg cgaaggcatc gctctgcagc gcgccgagat cgtgaagggc ctgacgaact
      121 ccatcgagga gctgaagaag tcgtaccccg gcgcctcgga agaggccatc atgaacatgc
      181 tcatgatgaa ccagtggctg gaagccctca ccgccatcgg cgccaagtcc ggtgccacga
      241 ccatcttcgt gccggcctcg ccgcagggca tgaccgactt ccgccagcag atcatggaag
      301 gtctggtggg ttcggtgaag ggcgccgcca agagctgatc caaggtttgt cggtgcgctg
      361 aagcgtaagc tccggcgcat cggcagccac aacatcgaga ggagcgtcgg tcgcaagact
      421 ggcgctcctc tttcctttct tttttagatc tatagagagg aaaaactatg gaaattccca
      481 acttcattgc catccccgcc ctgcttcttt tcgcggcggc agtacttttc atcaccgtgc
      541 gctgcatcaa gcagggcaag gtcggtcccg aatacggact gaaaatcaga atcaaaaaga
      601 aaaaggactg acagcggcag caattgccgg atttgacacc cggggcgaag cacgtcttcc
      661 tccgggctca agtttctttc ttttttggcg caatagttga ccggtctatt tgcctgcttc
      721 aaaaaatgaa cggctggagt aattagattg caaattattg tccttaatca atcgcgccca
      781 aatttcctct tcctctttgc tccggattcc aggtttaaag ccaaaccgca aataaagctt
      841 tatcgctggc agcctcacag tgcgggtcca cagataggag cgcgtttcgc cgagcaaagc
      901 aaatctctca aggactgagg aaattagggc tctgccaatt cccattcctt gatattccgg
      961 caatacggca acccagcgga ttcgtcccga ctgcgcttcc cagtacttat tgtccgacca
     1021 tgccgttgcc gtaccaatta tctggccgtt cttttcaatg tagagctggc gttcagaaag
     1081 ctcatcaatc ccttcaccaa actgcatctc aaaagttgaa ttatcaaatt cgttataatg
     1141 gtctgctttc ttttgcaaat ctagccagtg ccgctcatca ccggaacgat aggttctgac
     1201 cgtgaatcca cccggcaaac agaacctcgg aacctcatca aacgattcca aagtcattgc
     1261 aatgagtgcg ttattctcgt gcctatccac cggtcatcca aactgctttt gctttgatta
     1321 tttcatgtga tgaggaaaac aaagcaagcg ggaaagcttt tctttttgcc caccgctagc
     1381 cgaccggtct attattctcc ggtaaaaaag aaaggctccc tgaactttac cggctaggcg
     1441 aaccggttcc ggtgaggcgg catccatgcc actccaccag ggaaaagaaa gctccagtct
     1501 aatcgaaagc agagccggaa tcagattcaa cccctttcaa tccgacaaag gcaagactga
     1561 acaggaagac tcccagagcc agaagcaatg ccgccagggc accatacgag ccgccggtat
     1621 aagcacccag aggtagaagc gcaagagcca gcgcccttgc ccagtggcga aagcgatgga
     1681 cggc
//
LOCUS       DAOQNZ010001028         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_54105_length_1683_cov_3.673833, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001028 DAOQNZ010000000
VERSION     DAOQNZ010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54105_length_1683_cov_3.673833"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="mnmA"
                     /locus_tag="PKW73_16030"
     CDS             <1..1018
                     /gene="mnmA"
                     /locus_tag="PKW73_16030"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNA74859.1"
                     /translation="VDSSVTAYLMMEAGYDVVGVTGWLIKSGSRCCDTGMIDAARVCE
                     DLGIDHHAVDLRELFKNEIIDQFHESYARARTPLPCSLCNTLIKWGALMNYGRKLLNA
                     RYIATGHYARIVDTESGRRLCKAKDEKKDQSYVLWGLTLDQVNSTLLPLGDYDKGEIR
                     DIARKAGIVTADRPDSQDLCFIPRGTTTQEYLANFLTVEPGPLIHTVTGQVLGEHQGT
                     FNYTIGQRRGLGVAYSEPLYVTSIDPEMRVVYVGPKEALLRRELTASFVNWIMPSPPA
                     EPFQALAKIRYNSQAAPATIFPLAEGRVRVVFSEPQPAITPGQVLGIYDEENVYILGG
                     GWID"
     gene            1216..>1683
                     /locus_tag="PKW73_16035"
     CDS             1216..>1683
                     /locus_tag="PKW73_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74860.1"
                     /translation="MFYQKFSGIFKNLVRASLSQLTVVLLAAGTSALLLEAAPEAQAR
                     SKSASRTSVKKSGKVQSRRAVSSRSSSRSRAGGRTASKSSRRGSTASRRTRGGSTVAH
                     HGRHHKVAHHAPKTRYAYPIGLFMPRPPVFDTSPLSESEAERISDAFKNGTADG"
BASE COUNT          370 a          474 c          447 g          392 t
ORIGIN      
        1 tgttgattct tcggtaactg cctatttgat gatggaggcc ggctatgacg tggtcggcgt
       61 taccggctgg ttgatcaagt ccggcagccg ctgttgcgac acaggcatga tagatgcggc
      121 gagggtctgc gaagacctgg gcatagatca ccatgccgtc gatctgcgcg aactcttcaa
      181 aaacgaaatc atcgatcagt ttcacgagtc ttatgcccgg gcgcgcacac ccttgccctg
      241 tagtctttgc aatacgctga tcaagtgggg cgccctcatg aattacgggc gcaaactgtt
      301 gaatgctcgt tatatcgcca ccggacacta cgcccgtatc gtagatacgg agtcggggcg
      361 gcgcctctgc aaagccaagg acgagaaaaa agaccagtct tatgtgctct ggggcctgac
      421 tctcgatcaa gtcaattcaa cccttttacc gctgggtgat tatgacaagg gcgaaatcag
      481 agatattgcc agaaaagccg gtatcgtcac tgccgacaga ccggacagcc aggacctctg
      541 ctttatcccc agaggaacca ccacccagga atatctggct aattttctca ccgtggagcc
      601 gggacctttg attcacacag tcaccggtca agttttaggt gaacatcagg gcactttcaa
      661 ttacacaatc gggcaaaggc gtggtctggg agtggcatac agcgagccgc tttacgtcac
      721 ctccatcgat cctgaaatgc gggtcgttta tgtcggtccg aaagaggcgc ttctgcgccg
      781 ggagttgacg gcttctttcg tcaactggat catgccgtca ccaccggcgg aaccatttca
      841 agctcttgct aagattcgct acaattcgca ggcggcaccg gcaacgattt tcccattagc
      901 cgaaggtcgg gtgcgggtgg tcttttccga gccccagccc gccatcacac ccggacaggt
      961 gctgggaata tacgacgaag agaatgtata tattctgggt ggcggctgga tagactaggg
     1021 tgtcaagatg gatgtccgga gtatgacttc cccgccccgc cgaccgccgt ctcgaaaacc
     1081 gctccccatc taagatttca ccagccaacc ggctaaattt tgtttgctta cggggagttt
     1141 atcatttata atgaacccag gctttagttc agcatttcaa ttgggcgctg gcgtctcaca
     1201 tgagggcatc cacgtttgtt ttaccaaaaa ttctccggca tcttcaaaaa tttggtcagg
     1261 gcaagcctca gtcaattgac agtggtgttg cttgctgccg gtacttccgc cctactttta
     1321 gaagcggcac cggaggccca ggcgcgctcc aagtctgctt cccgcaccag cgttaaaaag
     1381 agcggcaaag ttcagagtcg tcgggccgta agctcacgtt ccagttcacg ctctcgagcc
     1441 ggggggcgca ctgcctctaa gagttctcgc cgcggcagca ctgcctccag gcgtactcgc
     1501 ggcggtagca cggttgctca tcatggtcgc caccataagg tcgcccatca tgctcccaag
     1561 acccgttatg cctatcccat cgggctcttc atgccgcgcc cgcccgtatt tgatactagc
     1621 cctctttctg agagtgaagc cgagcgtatt agcgatgctt tcaaaaatgg taccgccgat
     1681 ggt
//
LOCUS       DAOQNZ010001029         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_54141_length_1683_cov_2.449017, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001029 DAOQNZ010000000
VERSION     DAOQNZ010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54141_length_1683_cov_2.449017"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKW73_16040"
     CDS             <1..942
                     /locus_tag="PKW73_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA74861.1"
                     /translation="QADPPDHTRLRSLVNKAFTPAMVNEMRRHIEDIAEGLLLKYKAQ
                     GEMDLIADYAFVLPVTVIAEMLGIPPEDREHFKGWSHTLTEALEPSVAPQKLLAAAAA
                     NNALLDYLKPLIESRRKAPRQDLISALVQAEEEGNKLSEDELLANLVLLLVAGHETTV
                     NLIGNSALALLRHPQQFDLLKSQPELLEGAVDEFLRYDSPVQLIRRSASQTMELAGQT
                     IAADDTVLLLVGAANHDPLQFENPHQLDITRKNIKHLSFGAGIHHCLGSSLARAEGKI
                     ALAALMKHMPALALKSDDLEYRVPFALRGVKTMPVTF"
     gene            1010..>1683
                     /locus_tag="PKW73_16045"
     CDS             1010..>1683
                     /locus_tag="PKW73_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74862.1"
                     /translation="MRTTSSFLLFYMDRQSTGNDHSVEAKPSTNPAAEESAVLLALKG
                     SGAEKVKPSSPEFLTLTDPFTNRVSVNETDSPSSPRRWTMAIDLTASGNVEFQGERRP
                     TGADNKLQQILDLAERTKDTPVTLYVQAALPLPETVRADGSVMTNRNDQQVATYRLED
                     GQIKLVDQGPSQGFQQNLENLLQRASQRAGQGNLGLMIQSHGFAANGLGGDTGEASLD
                     ELEQTIR"
BASE COUNT          412 a          534 c          425 g          312 t
ORIGIN      
        1 caggccgatc cccccgacca tacccgcctg cgcagcctgg tgaacaaagc tttcacaccg
       61 gccatggtca acgaaatgcg caggcacatc gaagacattg ccgagggtct actcctcaaa
      121 tacaaggctc aaggcgagat ggacctaatt gccgactacg ccttcgtctt gcccgtcacc
      181 gtcattgcag agatgctggg cattcccccg gaagaccggg agcacttcaa aggctggtcc
      241 cataccctga ccgaggcgct ggaacccagc gtcgctccac agaaactcct ggcggcggcc
      301 gccgccaata atgccctcct ggactatctc aaacccctca tcgaaagtcg ccgcaaagct
      361 cccaggcaag acctgatcag cgctctggtg caggccgaag aggaaggcaa caaattatcc
      421 gaagacgagc tactggccaa tctggtacta ctgttagtgg cgggtcatga aaccacggtc
      481 aacctgatag gcaacagcgc cctggctctg ctacggcatc cgcaacaatt tgacctgctc
      541 aaatcccagc ctgaactgct ggaaggtgcc gtggacgaat tcctgcgcta tgacagcccc
      601 gtgcaactga ttcgcagaag cgccagccag accatggaac tggccggaca aaccatcgcc
      661 gcagacgata ccgtcttact cctggtggga gccgccaatc acgatcctct gcaatttgaa
      721 aacccgcacc agctagatat cacacgcaaa aatatcaagc acctctcttt cggagccggt
      781 attcaccatt gcctcggttc cagtttagcg agagcggaag gcaaaattgc cctggctgca
      841 ttaatgaaac acatgcccgc tcttgccttg aaaagcgacg atcttgaata cagggtgcct
      901 tttgccttgc gcggagtcaa gaccatgccc gtcactttct aaatccggcc tgctgcgttc
      961 cggttcccag attaattgcg cttgccgaaa ggaaaagccg gctttacact tgagaaccac
     1021 ctcttccttt ctccttttct acatggacag acaaagtaca ggcaatgacc actccgtgga
     1081 ggcaaagccc tctaccaatc cggctgcgga agagtccgct gtgctgctgg ccctcaaggg
     1141 cagcggcgcg gagaaggtaa agccgtcttc tcccgaattt ctcaccctta ccgatccctt
     1201 cacaaaccgt gtctcggtaa acgagacgga ttcaccgtcc tcacccaggc gctggaccat
     1261 ggccatagat ctgaccgcct ccggcaatgt ggaattccag ggcgaaagac gccccaccgg
     1321 cgccgacaat aagctgcaac aaattctcga cctggcggaa aggactaaag acacgccggt
     1381 caccctctat gtgcaagccg cgctgcccct gcctgaaacc gtgcgagcgg acggctcggt
     1441 catgaccaac cgcaacgatc aacaggtggc aacctaccgc ctggaagacg ggcagatcaa
     1501 actggtggac caggggccca gtcagggttt ccaacaaaat ctggaaaacc ttctgcagcg
     1561 cgcctcacaa agagccggtc agggcaacct cggtctcatg attcaatctc atggctttgc
     1621 cgccaatggt ctggggggtg ataccggcga ggccagcctg gatgagctgg aacaaaccat
     1681 ccg
//
LOCUS       DAOQNZ010001030         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_54144_length_1683_cov_2.408477, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001030 DAOQNZ010000000
VERSION     DAOQNZ010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54144_length_1683_cov_2.408477"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..639
                     /locus_tag="PKW73_16050"
     CDS             121..639
                     /locus_tag="PKW73_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74863.1"
                     /translation="MNFPSSNVDVSAHACDYAGRVSLLHVQKPDGGFRQVHGSRMSDG
                     YFCSLIASRPDIIRLLSPAQSGPVVQPSLRYDEPTIKDRGLFIVAADFGPSDWLIAIE
                     AQMASCDPGMAYHLLMGKLPEGIESVTQLVNLLNLFIGSAGLLDSLSGALRIRRYNLV
                     TRLTEGEPQWSF"
     gene            773..1141
                     /locus_tag="PKW73_16055"
     CDS             773..1141
                     /locus_tag="PKW73_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74864.1"
                     /translation="MTIPAYLIMSFLTACLVVFTLLAAGLLTLSRGKPLTIFVVTLVK
                     NTSETRAVGWYPEFAYAHEAVLSDLSEGNYYDHVVIEEVQASVHGEVVQAYWYALQEG
                     TWQSIAVPSWAQNIVNWGLG"
BASE COUNT          344 a          422 c          475 g          442 t
ORIGIN      
        1 tatgacggat taagtgttgc cgttgaggac agaaccgcgt cctaaaagcg gtgcggacag
       61 ggacgtcttt tagggctgaa gcccagagaa tcaactccag aaacgaaaac aggtcaaagt
      121 atgaacttcc cctccagcaa tgtcgacgtt tccgcccatg cctgcgatta tgccggcaga
      181 gtctcgctct tgcatgtgca gaaacctgat ggcggcttcc gtcaggtgca tggcagtcgc
      241 atgtccgacg gctacttctg cagcctgatc gcctctcgcc ccgacatcat ccggttgctc
      301 agtcccgctc agagtggtcc ggtcgtgcag ccgagcttgc gttatgacga gccgaccatc
      361 aaagatcgcg gcctcttcat cgtggcggcc gatttcggac cctccgactg gcttattgcc
      421 atcgaagcgc agatggctag ctgcgatccc ggtatggcct atcatctgct aatgggcaag
      481 cttccggagg gcatcgagtc ggtgacccag ctcgtcaacc tgctcaatct tttcatcggt
      541 tccgccgggc ttctcgatag cctttctgga gctttgcgca tcaggcggta caacctggtt
      601 accaggctga cggaaggcga accgcaatgg tctttctgaa tctggctcct tgagccacta
      661 gcgccttctg gaaggcgctt ttgctccagg gagtagttca aaaacatcct cggcttctgc
      721 tggactggga gccgaggttt aaggtatagc atcgataagg gtggtgccgc taatgactat
      781 tcccgcttat ctgatcatga gttttttgac tgcctgcctg gttgtgttca ctcttttggc
      841 agccggactt ttgactttgt cgaggggcaa gccactgacc atctttgtgg tcacccttgt
      901 taagaacacg agcgagactc gcgcagttgg gtggtatccg gagtttgcct atgcgcatga
      961 ggccgttttg tcagatttga gcgaaggcaa ctattacgac catgtcgtca tcgaggaagt
     1021 gcaggcgagc gtgcatggtg aggtggtgca ggcctactgg tacgcgcttc aggaaggtac
     1081 ctggcagtct atagctgttc catcttgggc gcaaaacatc gttaactggg ggcttggcta
     1141 gaccaattta gttcggaagc tggcggaaga aggagtttct atgagagtct ttcaggtctt
     1201 cgcccctgat gatcttactc tgctcgacgc atttctcacc aggtggaggt cgtcggaggc
     1261 cggaagacga ccgctttctt ccgcttttcg gtgggggaag aagaagctgc tgcgggagct
     1321 ttggaaagac agacccttca tgactgttta tcgcacaggt acagcgtggt aatggtgaat
     1381 tttctggagg gttttgccag ggatggcttt ccctcaagtt gcttctgcac tctctctttt
     1441 gccgaaaaga tacgataaaa actagtagaa ttggctttgg aaaaataccc cgtgtctgtg
     1501 agccgatttt agttgtttcc ctggttgctc gcaccaaaaa gcacctgctg tcgctttgat
     1561 ttttatcgac cctattggag ttatcctctg ccagtcagaa tggctgagtg ccggcagtta
     1621 gataattaga tcatcgcttt gttgccggtg tgaattgttg cagaaaatgg gtgagagccc
     1681 ggc
//
LOCUS       DAOQNZ010001031         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_54722_length_1675_cov_2.198765, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001031 DAOQNZ010000000
VERSION     DAOQNZ010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54722_length_1675_cov_2.198765"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PKW73_16060"
     CDS             complement(<1..635)
                     /locus_tag="PKW73_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA74865.1"
                     /translation="MGEEVETKPAKHCPACHAGYSADMRFCPQDGTQLKESSRPENGK
                     LTNGASEKNSSSTATDFDQELKLDDVHAESAAVPDNATRTIEITGNAASLRPPGTFAD
                     PSLELLGQLVEGRYEVTENIGKGGMSVVFKARDRRLKKNVAIKVLMPHLSADPLSVQR
                     FQQEATAASHLDHPNIVKVYNVGATESGLPFMAMDLLQGRALSSLIKEKGK"
     gene            766..1629
                     /locus_tag="PKW73_16065"
     CDS             766..1629
                     /locus_tag="PKW73_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74866.1"
                     /translation="MRRKLYSLCVSLLILPSALPDYAHAAPSVRASSSKTDSAKEQGR
                     GGPPAPGWELHQWHRMAGRIRLVSTKYGGKFETTHYTLLKQMPGDKMNFLNQQTRRYV
                     SYSSDAWRKKFNFYRKDPGRAKDAGFTEFSAWKRQGEEKIAGLKCVRYSRMRTNNLPP
                     PNDKTYTEEWSFCRELAVPPALIELYKKVLYLQYDAKLGFPMRVKEIREWNGKKARKV
                     ELSYDTLKYNKASFPQSFFEIGKDYKQAKDEMSVLMDEGADMMDGDMMGGDGSLGGLE
                     IKDELKRRLGN"
BASE COUNT          385 a          447 c          415 g          428 t
ORIGIN      
        1 aatttgcctt tttccttaat gagagaagag agcgctcgtc cctgcagcaa atccatcgcc
       61 atgaacggca gcccgctttc cgtggcgccg acgttgtata ccttgacgat attggggtgg
      121 tcgaggtgac tggctgccgt tgcttcttgt tggaatcttt gtacgctcag cgggtcggct
      181 gataaatgtg gcatcaagac tttgatggcc acgttctttt tcaatcgacg gtcacgcgct
      241 ttaaatacaa cgctcatgcc gcctttgccg atattttcag tcacctcata gcgaccttca
      301 accagttgcc cgagcaattc caggcttgga tcggcgaatg tacctggcgg tctgagcgaa
      361 gctgcgttgc cggtgatttc aatagtcctt gtcgcattat ccggaaccgc cgcggattcg
      421 gcgtgcacat cgtccagttt cagctcttgg tcaaagtcag tggctgtcga ggaactgttt
      481 ttttcgctcg cgccattggt aagtttgcca ttctcggggc gcgaggattc ctttagctga
      541 gttccatcct gggggcaaaa acgcatatcg gcagaataac cggcgtggca ggccggacag
      601 tgtttggcgg gttttgtttc gacttcttcc cccacttttt tcagctcttc tggcagtctc
      661 cggttcccca gttcctacat tcccccttta cgatcttcta tctctcagaa cgtagtatca
      721 tgcctggcgg actacatttt cgaagccttt cctccagtgc cgaccatgcg ccgcaaactt
      781 tactcactct gcgtctctct tctcatcttg ccttctgccc tgcctgatta tgctcatgcc
      841 gctccatctg tcagagcctc aagcagcaag accgactcag ccaaagagca gggtcgcggc
      901 ggtccacctg cccccggctg ggaattgcac cagtggcaca ggatggctgg tcggatcaga
      961 ttagtctcta ccaagtacgg cggaaagttt gaaaccaccc actatacgct tctaaagcaa
     1021 atgcctggcg acaagatgaa tttcttgaat cagcaaacca ggcgttacgt aagttatagc
     1081 tctgacgcct ggcgcaagaa attcaacttt taccgcaaag atccaggacg ggcaaaggac
     1141 gccggtttta ccgaattttc tgcttggaaa cggcagggtg aagaaaaaat tgctggtctg
     1201 aagtgcgtcc gttattcgcg catgcgcacg aataatttgc caccgcctaa tgacaagact
     1261 tacaccgagg aatggtcttt ctgccgcgag cttgctgttc cgcccgcctt gatcgagctc
     1321 tacaaaaagg tcctctattt gcagtacgac gccaagctcg gttttccgat gcgagtcaag
     1381 gagattcgcg agtggaatgg aaagaaagcc agaaaagtcg aactaagtta cgacactctc
     1441 aaatacaaca aagccagttt cccgcaatca tttttcgaga ttggcaagga ctacaaacaa
     1501 gccaaagacg agatgtccgt tctcatggat gagggcgcag acatgatgga cggcgatatg
     1561 atgggcggcg acggctctct gggcggtctt gaaataaagg acgaactcaa gcgccgatta
     1621 ggcaattgat tcttaaattt gccaaactta aaatcctatc ctgaccctgt gaatc
//
LOCUS       DAOQNZ010001032         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_54778_length_1674_cov_2.676343, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001032 DAOQNZ010000000
VERSION     DAOQNZ010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54778_length_1674_cov_2.676343"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..>1674
                     /locus_tag="PKW73_16070"
     CDS             238..>1674
                     /locus_tag="PKW73_16070"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNA74867.1"
                     /translation="MSLLPKTRLLPLAVLSLLLVQTPGLYFCQAALAQDAGMVAEPKA
                     EGKADPSQYVVDEVRIEGNRLIPTEDIAGVVKTRKGDRFNREQVMEDLKAINGMGYFD
                     DRALEVNPELTGSGVLLKIRVQENAPITQFSIRGNEAVSTEEISKLFNDQLGKPQNIN
                     ALSSAIEKVEQAYHDRGFVLAKVTGVKDDPDGSVELEISEGSVDSIEIVGNRKTKDFI
                     IRNAIKLKPGSVYNEKQLTADLRKLYGNGYFQDIRRSLVPSAANPEKYTLKVEVDEKR
                     TASVGMGGGVDSVAGPFGSLSFSDGNFRGKGQVISLSSQMGSGTLNNMSNSINNAGTS
                     FMPTGKTYNLEATFIEPSLKGTNTSMAITGFGRNMGSMFIDQAMQRTLGASVVFSKPL
                     KGNWSGNLGLIGENTTLRNMANVAENQSILGQMSARALATGLAASDAQAAAVAGQYRA
                     DQLKGGAFLTVNPSLTRDTRDSAIDPTRG"
BASE COUNT          398 a          456 c          442 g          378 t
ORIGIN      
        1 gtgggttggt tcgagaatca ggagtagcca aaatagacgg gcttttgggt ttgcttcata
       61 agctggtctc ttgaaataga accgccgctt tcctcttttg gaagcggcgg tttgcttttg
      121 tgcatgccaa gtggttaact cgggctaagg ttgcatccct tcaagaaaca ccttggcatg
      181 ctccttcgtc atgttaaaat gctagcctgt tgcgactatt ttccaagtga gtagtaagtg
      241 agccttcttc ccaaaactcg attgctacca ctggcggtac tgtcactact tctggtgcag
      301 acgccggggc tatatttctg tcaggcggcc ctggctcaag acgcaggcat ggtggccgag
      361 ccgaaggcgg aaggcaaagc cgatcccagc cagtatgtcg tcgatgaagt gcgcattgaa
      421 ggcaaccgac ttattcccac cgaagatatt gccggtgtgg tcaagacccg caagggtgat
      481 agattcaacc gtgagcaagt catggaagat ctcaaggcca ttaacggcat gggctatttc
      541 gatgaccggg cccttgaggt taatccagag ctgacaggca gcggcgtact gctcaaaatc
      601 agggtgcaag aaaacgcgcc cattacacag ttttccattc gcggcaatga agctgtttct
      661 actgaagaga taagcaaact cttcaacgat cagctcggca agccgcagaa catcaacgca
      721 ctttcttccg ctatcgagaa agtcgagcag gcttatcacg atcgtggttt cgtgctggcc
      781 aaggtaaccg gcgtcaaaga cgatccggac ggttccgtag agctggagat atccgaaggc
      841 tcggtggatt ccattgagat tgtcggcaac cgcaaaacca aagactttat cattcgcaat
      901 gccatcaaac tcaagcccgg ttccgtctac aacgagaagc aactaaccgc cgatctgcgc
      961 aagctctacg gcaacggata ttttcaagac atacgccgct ccctggttcc ctctgccgcc
     1021 aatcctgaga agtacactct caaagtggaa gttgacgaga agcgcacagc ttcagtcggt
     1081 atgggcggcg gtgtagactc cgtggccgga cctttcggct ccctcagttt ctccgacggc
     1141 aacttccggg gtaaggggca ggtcatcagc ctcagttcgc agatgggctc cggcactctc
     1201 aataacatga gtaattccat caacaatgcc ggcaccagtt tcatgcccac cggtaaaacc
     1261 tataacctgg aagccacctt catcgagccc agcctgaagg gcaccaatac ttccatggcc
     1321 atcaccggct ttggacgcaa tatgggtagt atgttcatcg accaggccat gcagcgcacc
     1381 ctcggtgcca gcgttgtctt cagcaaaccg ctcaagggca actggtccgg taatcttggc
     1441 ttgatcggcg aaaacaccac tttgcgcaat atggctaatg tggctgaaaa tcagagcatt
     1501 ttggggcaga tgagcgccag ggctctggct accggattgg ccgcttctga tgcccaggcg
     1561 gcggcagtgg ccggtcagta tcgagccgat cagctcaagg gtggtgcatt tcttactgtt
     1621 aacccctcac ttacccgtga tacccgggat agtgccatcg accccacccg cggt
//
LOCUS       DAOQNZ010001033         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_54808_length_1673_cov_4.648949, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001033 DAOQNZ010000000
VERSION     DAOQNZ010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54808_length_1673_cov_4.648949"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..787)
                     /locus_tag="PKW73_16075"
     CDS             complement(77..787)
                     /locus_tag="PKW73_16075"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNA74868.1"
                     /translation="MPSIDLDQSDRQVQWRCPYPDCAGFSLPIESSAVAVPVCPVCLR
                     PFTPRSAVHFQEMQKKVRSNPQSPSIALPAASDLVVVIEDLRSLHNVGAVFRTADGAG
                     FSAIYLCGITGLPPRKEIAKVSLGAEDFVPWQFRPFIAGCLQELKSANFCLVALEKND
                     NSEPLYRCLQEGKLRRPLALVVGNEVSGVSREAQYYCDQVCHLSMRGQKESLNVSVAF
                     GAAAYLISEFFAFPEHTI"
     gene            complement(787..1410)
                     /locus_tag="PKW73_16080"
     CDS             complement(787..1410)
                     /locus_tag="PKW73_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74869.1"
                     /translation="MSLVWNDLKQSAEKAMEANDWSTALSDWEAALKEAESFPDGDGR
                     QVATLEGLARTRFGRGDHDEAEFLYKRTLVIRRDEQGKDHKDVASVERNLGVVLFTAG
                     RYSEAQSHFEKALNLKKTAFGDAHIEVGRTIYLLALACHAQGHYDEADTHYRKSLDIK
                     NKALGNNSPELINLLRNYADLLRKTSRDSIAGQMEQFAKGIEAKQKH"
BASE COUNT          390 a          472 c          431 g          380 t
ORIGIN      
        1 ccccgccaag ccggactggg taggtctgcc cggcaaggaa ggcagtttcg ggcagcttcc
       61 aggctcaaat ttcctctcaa atcgtgtgct cgggaaaggc gaagaattca ctgatcagat
      121 aggccgccgc cccaaaagcg acggaaacat tcaaagattc cttctgccct ctcatgctca
      181 agtgacagac ctgatcgcag taatactggg cttctctgga cacaccactg acttcattgc
      241 ccaccaccag agccagtggt cgcctgagct tgccttcctg caggcatcga tagaggggct
      301 cagaattgtc attcttttcg agagccacca ggcaaaaatt cgccgacttt aattcctgca
      361 ggcaaccggc gatgaatggt cgaaattgcc agggtacgaa gtcttccgcc cccagcgata
      421 ctttggcgat ttctttgcgc ggcggcaaac cggtgatgcc gcacaggtaa atagcagaga
      481 agccggcacc atcggcggtt ctaaatacgg caccaacatt gtgcaaactg cgcagatcct
      541 cgattaccac caccaggtcg ctggcggccg gcaaagcaat agaaggcgac tggggattgg
      601 accttacctt cttctgcatt tcctgaaagt gaacggcgga gcgcggtgtg aaagggcgca
      661 ggcaaacagg acagaccggc accgccaccg cactactttc aattggaaga gaaaacccgg
      721 cacaatcagg gtaaggacaa cgccactgaa cctgacggtc ggactggtca agatcaatgg
      781 acggcattaa tgcttctgct tcgcttctat acccttggca aactgttcca tctgcccggc
      841 aatactgtct ctgctggttt tgcggagaag gtcggcataa tttcgcaaga gattgatcag
      901 ttcggggctg ttgtttccta gcgccttgtt tttaatatcg agacttttac gataatgggt
      961 atcggcttca tcgtaatgcc cctgagcatg gcaggccagg gccaacagat agatggtccg
     1021 tcctacttcg atatgggcat caccaaaggc ggtcttcttg aggttgagcg ccttctcgaa
     1081 atggctctga gcctcagaat accgaccagc agtgaagaga accacgccca gattgcgctc
     1141 tacggatgct acatctttgt ggtctttgcc ctgttcatcc cgcctgatca cgagtgtacg
     1201 cttgtagagg aattccgctt catcgtggtc gccgcggccg aatctcgtgc gggccaaccc
     1261 ctccagggtg gcaacctgcc tgccgtcgcc gtccgggaaa ctctcagctt ccttcaatgc
     1321 tgcttcccag tcgctcaaag ccgtggacca gtcattagcc tccattgcct tttcagccga
     1381 ctgcttgaga tcgttccaca ctaaggacat ggctaccccc aatttgctct ctaccaaggg
     1441 gcattctacc ctcttgggta gccctccaat cgccaagggg taagggactt ttaccggtcc
     1501 gggcaggaca ggttggaatt ctttagaatt ttctagctct caccgggggg aaacttgttt
     1561 agttttgttg ccgaagcgct aaactaaaca aaacaccagg gttggggctg ccggatgcta
     1621 tcaaggcagc agactcaaag gcatctctaa ccacagccag gtcaatgcaa taa
//
LOCUS       DAOQNZ010001034         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_55023_length_1670_cov_3.582663, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001034 DAOQNZ010000000
VERSION     DAOQNZ010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55023_length_1670_cov_3.582663"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKW73_16085"
     CDS             complement(<1..340)
                     /locus_tag="PKW73_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74870.1"
                     /translation="MAQPDRDKSGPPVLAPGKTTVFEKPIDTNVALAVCYLPLGIVSL
                     AACVLVFMSKSDNNRLARYHAVQAALIAVLVLVVTGVVGVVGAIPFIGILAGLLQIVI
                     AMGYFIFSAKM"
     gene            657..1577
                     /locus_tag="PKW73_16090"
     CDS             657..1577
                     /locus_tag="PKW73_16090"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74871.1"
                     /translation="MRSRKAYHSPVRKELLVAKDSLIGVILDDRYEILSEVGRGALGV
                     VYKARHLVMEKTVAVKVLFGEVDATKDETNFLRFQREAQAASSMSHPNIVTVYDFGFS
                     AQKTPYLVMDFIEGVNLKEILSRHQRLPVERGVHIFLQMCSALEHAHSKGILHRDIKP
                     ENVVLLKTSWNPEFVKLVDFGIAKYVNEPRNNSKKLTMDGQVLGTPAYMSPEQVMGDR
                     LDARSDIYCMGVLMYHTISGKLPILGSTSADTMSRHITDKPKEFAEICPGLPVPFKLQ
                     RTIMKALKKHPQDRHQSMKELYVELENYRD"
BASE COUNT          453 a          475 c          395 g          347 t
ORIGIN      
        1 ccattttggc actgaagatg aagtagccca tggcgataac aatctgcaaa agcccggcta
       61 agattccaat aaaaggaatg gcgccgacaa cgcccaccac accggtgaca acaagaacca
      121 gcacggcaat gagagcagct tgcacggcat ggtaacgagc caggcggtta ttgtctgatt
      181 tggacatgaa gaccagcacg caggctgcca gactgactat acccagaggc agataacaaa
      241 cggccagggc cacattagta tcaattggtt tctcgaatac cgtggtcttg cccggcgcca
      301 acaccggcgg acccgactta tctctatcag gttgtgccat aaagattaaa atactccctt
      361 cagtccgttc cgagctctat tttcccagac ttaccctgac caggatctcc ggtgacgcct
      421 ccttaagcct agcagattgg aacacccctg cgcttgctgt agacctgcct atagagctag
      481 cggcggcatc gcaactgcca gtggtatcat cgccgatcgc cggaagccaa gcagaagccg
      541 gacccaaaaa tagtacataa aaagaccaat ttaatatcgg tccccccact cttccgaaca
      601 ggttaaaatc taaccttggc tccatcgaga aaagcccggc tcggttccaa gtgccggtgc
      661 gatcccgaaa agcctatcac tcaccggttc gtaaggaatt attggtggcc aaagactcac
      721 taatcggagt catacttgac gaccgctacg agatcttgtc ggaagtaggc aggggtgccc
      781 tcggcgtggt ctacaaagcc cgccacctgg taatggagaa gaccgtggcc gttaaggtcc
      841 tcttcggcga ggtggacgcg accaaggatg aaaccaactt tttaaggttt caaagagaag
      901 ctcaagcggc aagttccatg agccatccaa acatagtgac agtctacgac tttggctttt
      961 ccgcccagaa aactccctat ctggtcatgg atttcataga aggggtgaac ctcaaagaga
     1021 ttctctctcg tcaccagcgg ctgcctgtgg agcggggcgt gcacatattt ctgcaaatgt
     1081 gcagcgccct cgagcacgcc cattccaaag gcattttgca ccgcgacatc aaacccgaaa
     1141 acgttgtgct gctcaaaaca agctggaatc cggaattcgt caaactggtt gatttcggca
     1201 ttgctaaata cgtgaacgaa ccccgcaaca actcgaaaaa actgaccatg gacggtcagg
     1261 tgttgggtac tccggcttac atgagccccg aacaggtcat gggtgatcgc ctcgacgctc
     1321 gctccgacat ctattgtatg ggtgtgctca tgtaccacac aatttcaggc aaactaccca
     1381 tactgggtag cacttccgcc gataccatga gcaggcacat caccgacaaa cccaaggaat
     1441 tcgcagaaat ctgccccggt ctgccggttc ccttcaagtt gcagcggacc atcatgaaag
     1501 cgctcaaaaa gcatccgcaa gaccgccatc agtccatgaa agagctttat gtggagttgg
     1561 aaaactaccg tgactgacac acgccaatga acgtcaagac tctcctcatt atcttcacgg
     1621 tctgcttgat cgcctccata ccctttgtca caaaagggca cgatgacacc
//
LOCUS       DAOQNZ010001035         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_55453_length_1664_cov_3.597887, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001035 DAOQNZ010000000
VERSION     DAOQNZ010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55453_length_1664_cov_3.597887"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..415
                     /locus_tag="PKW73_16095"
     CDS             113..415
                     /locus_tag="PKW73_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74872.1"
                     /translation="MDDRLKELAEMRYGQSEYLKVLFDLALEDNWFDLQHMIQHDMAK
                     AILADYSYEKGLGYLNQEVFFDFWEEVIEVGWSIFCRHTGLSRERVDKALANLREH"
     gene            complement(429..731)
                     /locus_tag="PKW73_16100"
     CDS             complement(429..731)
                     /locus_tag="PKW73_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74873.1"
                     /translation="MQQEDRELKVPENVSYSKLLSALKTARTAVVSPVDGADAEYVMD
                     LAHNLRQGHIQDVMDLREERLGQITGARGPAYRGFPEFSSSLEPAAMNFFLKQSGE"
     gene            complement(993..>1664)
                     /locus_tag="PKW73_16105"
     CDS             complement(993..>1664)
                     /locus_tag="PKW73_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNA74874.1"
                     /translation="RAGKPVSSTRGEEETGPKPERYLRAKETFVYQFVPQMQSQSTAY
                     YHPQIMHLKQRQQGLYGALIVHPRLAAHRADQDLSLFFTRVSVKGNSEKALPAQTGAK
                     AAETAERQVFLVNGKTAPYIPALQVTAGKRIRLRLFNLTEESIPLHLSGHRLEVVGTN
                     GSDPLEPHTMRDTVTLASGDRLDVEFTADNPGVWSLASDRLSQVTDKQGNFPHGLAIV
                     VKYKQ"
BASE COUNT          336 a          430 c          476 g          422 t
ORIGIN      
        1 ccggagatgg catgggcagt gagcgccgcc gccatcgtga ttttcctttt gtaatcaccc
       61 aagttgtgat agttttgaga gtgaaatcta tttcaatgtg acttgatttg ccatggacga
      121 tagactgaaa gaactggctg aaatgaggta tgggcaaagc gagtacctga aggtactctt
      181 tgacctggcc ctggaggaca actggttcga tttgcagcat atgatccagc acgacatggc
      241 caaagccatc ctggccgact actcctacga aaaagggctg ggctacctca atcaggaggt
      301 cttcttcgat ttctgggaag aagtcattga agtgggctgg tcaatatttt gccgtcacac
      361 gggcttgagt cgagagcggg tcgataaggc cctggccaat ttgcgagaac actgatggtg
      421 acctgttctt attcgccgct ttgtttgagg aagaagttca tagcggccgg ttcaaggctg
      481 gatgagaact cagggaagcc tctataagcc ggtccgcgag cccctgtaat ttgcccgagg
      541 cgctcttccc gcaagtccat cacatcctgg atgtgtccct gtctgagatt gtgggccagg
      601 tccatgacgt attcggcgtc ggcgccgtcc accgggctca cgactgctgt ccgtgcagtt
      661 ttgagggcac tcaatagttt gctgtaagag acattttcag gaactttcaa ctctcggtct
      721 tcttgttgca tggattattc tctcggggca atgactattt gactggtaag ggaaacgggc
      781 caaccggctt gttatcagaa tagccccgga tttttaacat gcaagctaca aggcatttca
      841 tattcatccg ccccgggccc tgccatctct tgcttgtgcc ggtgctaagc agcccgggct
      901 gtcagtttgc ctcgggtgag tagatagttt gtagatttgt aaggcgctag atggcagaga
      961 atttaactga ccggaagagc gccctgagcc ctctactgct tgtatttgac cacgatggcc
     1021 agaccgtggg ggaaattgcc ctgcttgtcc gtaacctggg agagtctgtc ggaagccagc
     1081 gaccagacgc cgggattgtc ggcggtgaat tccacatcca gacgatcgcc gctggctaag
     1141 gtgacagtgt cgcgcatggt gtgcggctcc agcgggtcag agccgttcgt ccccactact
     1201 tccaggcggt gcccgctcag atgcaaggga atggactctt ccgtcaggtt gaagagcctt
     1261 aatctgatgc gctttcctgc cgtcacctgc agagccggga tgtatggggc agtcttgccg
     1321 ttgacgagga atacctgccg ttccgccgtt tcagccgcct ttgctccggt ctgggccggc
     1381 aaagcctttt cactgtttcc tttcaccgac accctggtga aaaacaagct cagatcctgg
     1441 tctgcccggt gggccgccag gcgcggatgc actatcaagg ctccatagag accctgttgc
     1501 ctttgtttca gatgcatgat ctggggatgg tagtaagccg tgctctggga ctgcatttga
     1561 ggcacaaact ggtagacaaa tgtttctttg gcgcgcaagt agcgttcggg cttgggtccg
     1621 gtctcctctt cgcctctggt actgctgaca ggtttgcccg ccct
//
LOCUS       DAOQNZ010001036         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_55486_length_1664_cov_2.359229, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001036 DAOQNZ010000000
VERSION     DAOQNZ010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55486_length_1664_cov_2.359229"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..>1664
                     /locus_tag="PKW73_16110"
     CDS             195..>1664
                     /locus_tag="PKW73_16110"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNA74875.1"
                     /translation="MADDRTAVKDHAKEAAPPKDHGGDNAQAHIVQQTKGTEALAIIK
                     DTDKGKTAQGLAHVELYDSTAAAAKTAEKPAGKDNGKPEETSAKTGNAGEKSATAGEK
                     PEAKAAAPETKPEVTKYEVKKDDTLSQIVEKQLPKNEGESHKDYVKRLYEKVGEVAKH
                     NKLDDPNKLEIGQKLEIPQDKKPEQAPAASGDGKTPVPQEQSRQNTDGSAVAALPEKV
                     QTPEEKAAAEKAAAEKAAADKARAEKAAEAIDQAANGGFLGIGTDKKAIEEQLKGKSK
                     EELQAIDEAYKKRTGISLKDELRDELSGSDLTRALNLADGHNDDAARINVALEEHKEW
                     GLGARSNSNIEKDLRDTISTLNSKQVEELDRTYQERYGKSLKDVLKDDPNLPKETKEA
                     LGIYLKGTDKMTPADTLAVAQIGLDSKNTDIFQEAFRGASPEARAQFLSDGGEDKVKK
                     AFGSASYDDTTGETTYSRNSEVDRAMDYVKQGKLDASTKI"
BASE COUNT          545 a          455 c          438 g          226 t
ORIGIN      
        1 ccacaaggct cgcagtcggc agcaaacagc aaaataggtc gtacagacac cgaaacggcc
       61 agaccaatat cccttactta ccggccggcg cccaacaatt aagataaatc cggcacgaat
      121 ggtgtccggc gaagtttagg gaaggtatat ataagggaac attgttacgg attactacgt
      181 acccctgagc attaatggct gacgacagaa cagcggtgaa ggaccacgcc aaggaggcgg
      241 cgccccccaa agaccatggg ggcgacaacg ctcaggcgca tatcgttcag cagaccaaag
      301 gcacagaagc cctggccatc atcaaggaca cggataaggg aaagactgcc cagggactgg
      361 cccatgtaga actctatgat tccaccgccg cagcggcaaa aaccgcagag aagccggctg
      421 gaaaagataa cggaaaaccg gaagagacca gcgccaagac tggaaacgcc ggtgagaagt
      481 ctgcaactgc cggcgaaaag ccggaagcta aagcagccgc cccggaaacc aaaccggaag
      541 tcaccaagta cgaagtcaag aaagacgaca ccctcagcca gattgttgag aaacagcttc
      601 ctaaaaacga aggcgagagc cacaaagact acgtcaagcg cctctacgaa aaggtagggg
      661 aagtagccaa acataacaaa ttagacgatc caaacaaact ggaaatcggt cagaagttag
      721 aaattcccca ggataaaaag cccgagcaag caccagcagc ttcaggagac ggcaaaacac
      781 cggttcccca ggaacaaagc cgacaaaata cagacggtag tgcggttgcc gccctaccgg
      841 aaaaagtgca aactccggaa gagaaagcag cagcagaaaa agcggcagca gagaaggcgg
      901 cggcagacaa ggcaagagcg gaaaaagcag ccgaagccat cgaccaggcc gccaacggtg
      961 gtttcctcgg catcggcaca gacaaaaaag ccatcgagga gcagttaaaa ggtaagtcga
     1021 aggaagaact tcaggccatc gatgaggcct acaaaaagag aaccggcatc agccttaaag
     1081 acgaattgcg tgatgaactt tccggttccg acctgaccag agccctgaac ctggccgacg
     1141 gacacaatga cgacgccgcc cgcatcaacg ttgcactgga agagcataaa gaatggggac
     1201 ttggtgctcg ttccaatagc aatatcgaaa aagacctgcg cgacaccatc tcgaccttga
     1261 actccaagca agtagaagag ttggaccgaa cctaccagga gcgctacggc aaatcgctga
     1321 aggatgttct caaagacgat ccaaatctgc cgaaagaaac caaagaggct ctcggtatct
     1381 acctgaaggg caccgacaaa atgacgccgg ccgacaccct tgccgtggcc caaatcggtc
     1441 tcgattcgaa gaataccgac attttccagg aagctttccg cggagcatcc ccagaagcca
     1501 gggcccagtt cctcagcgat ggcggtgaag acaaagtcaa gaaggccttc ggctcggcct
     1561 cctatgatga tactaccggc gaaaccacct actcgcgcaa cagcgaagtg gaccgcgcca
     1621 tggattatgt caaacagggc aaactggacg cctccaccaa gatt
//
LOCUS       DAOQNZ010001037         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_55530_length_1663_cov_3.229478, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001037 DAOQNZ010000000
VERSION     DAOQNZ010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55530_length_1663_cov_3.229478"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1500
                     /locus_tag="PKW73_16115"
     CDS             115..1500
                     /locus_tag="PKW73_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA74876.1"
                     /translation="MAEVAESKKVAVVGGGLSGLSAAVNLARHGLAVTLFERSAREGG
                     RAQTTVKDGYHINFGPHAFYLGGPGRPFLKELGIEPAGSPPPISNAVAFYRGKNPILP
                     LSASAIFKTDLFGLGDKIRLFKFMLKLNSIDEKEFESLTVDQWIEKDPILGAGGDALK
                     KFVRTLIQLSTYAGDTDKMSARAGLHQLKAAVSSGVSYLHGGWEQMVDALVCRAEDLQ
                     VNLVVATEVTEVSRNKETGELKVSFIDRIRGPVSENFAGVILAAPPNMMKKFLAVEDV
                     AAGHTALNLGGDELLDVRAACLDICLSSLPRPDMTYSLGIDEPLYYSVHSSAARLTPS
                     NGALIHTAFYLKHGETGTPEIEKRLEEMMDTLQPGWRDKLVYKRFLPNISVVHKLLIV
                     KDGKLAGYWTEETDEPGVFRAGDWVGTEQLADASLASAKRSSELLISYIENAQVKSEV
                     RRRANLSALSI"
BASE COUNT          411 a          385 c          447 g          420 t
ORIGIN      
        1 aaaatcatgg acaaatattg tccatgaatc tatcatatta gcagggactg aagagcggct
       61 aaaaatcgac gcaattaacc gagcttgatc ggtccaggac tggagggcaa gacaatggct
      121 gaagtggcgg aaagtaaaaa ggtggcggta gttggtggcg gtctgtccgg gctttcggcg
      181 gctgttaacc tggcgcggca cggtctggcg gtgacattgt tcgagcgctc tgcgcgcgaa
      241 ggcggccgcg ctcaaacgac ggtaaaggac ggctatcaca taaatttcgg tcctcatgct
      301 ttttatcttg gcgggcccgg gcgccctttt ctcaaagaat tgggcataga gccagctggc
      361 agtccgccgc ccatatcgaa tgcagtcgcc ttctatagag gtaagaatcc aatcttgccg
      421 ctctcggcaa gtgcgatttt taagaccgat ctatttggtc tgggcgacaa gattcgactg
      481 ttcaaattca tgttgaaact taattcgatc gacgagaaag aatttgaatc gctcaccgtt
      541 gatcagtgga tagaaaaaga tcctatcctg ggtgctggcg gcgatgcgct aaaaaaattc
      601 gtgcgaactc ttattcaact ttcaacttat gcgggcgata cagacaagat gtctgccagg
      661 gctggacttc atcaactcaa ggcagctgtg tcaagcggtg tctcgtatct gcacggagga
      721 tgggagcaaa tggttgatgc tctggtttgc agagcagaag atttgcaggt taatttggtt
      781 gttgccacag aagtgactga agtttccaga aataaagaaa ctggagaact gaaagtttca
      841 tttatcgaca gaatcagagg tccggtaagc gaaaattttg caggcgttat tcttgctgcg
      901 ccaccaaata tgatgaagaa gtttcttgcc gtggaagatg ttgccgccgg gcatactgcg
      961 cttaatttag gtggcgatga gcttctcgat gtgagagctg cttgcctgga tatttgcctc
     1021 tctagtttgc ctcggccgga catgacctac tcgcttggaa ttgacgagcc tctctattat
     1081 tcggttcatt cttctgctgc aagactcaca ccttctaatg gtgcactcat tcatacagct
     1141 ttctacttga agcacggtga aacaggtacg ccggagatcg agaagcgtct ggaagaaatg
     1201 atggacactc ttcagccggg ctggagagac aagctcgttt acaagcgctt tttgccgaat
     1261 atttctgttg tgcacaaact tctaatagtg aaagatggca agctagccgg atactggact
     1321 gaggaaaccg atgagccggg cgtttttcgc gcgggtgatt gggtcggtac cgaacaactg
     1381 gcggacgcct cacttgcaag cgccaagcga agttccgaac tgctcatttc ttatatcgaa
     1441 aacgctcagg tcaaatcaga agtgcgccga cgcgccaatc tgtccgcact tagcatatag
     1501 tgcgctttaa tagacaaaac gtccctggcg catcttatcc tggattgcac gcagcagcgc
     1561 ttcacgctgt tcgtagctca ggttgctcaa gttaatgggc acccgttgca gtcgcgctcc
     1621 aggaatgaat gctgctacag gcggttcttc tacattgcgc ccc
//
LOCUS       DAOQNZ010001038         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_55549_length_1663_cov_2.722637, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001038 DAOQNZ010000000
VERSION     DAOQNZ010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55549_length_1663_cov_2.722637"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1076..>1663
                     /locus_tag="PKW73_16120"
     CDS             1076..>1663
                     /locus_tag="PKW73_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74877.1"
                     /translation="MTSEAAAFYLLTLPAFWLIGRDYLTRRGDLSPMVAMHLVINQLV
                     TTEQVPYVDFLDLAQPIIYELYRLPALLNDALGFCGMPMRLEEVVKLIFIVLTALSSV
                     ACFLILGLARSSLQARVGTEPAEKAEEEALTEALSLSQVAFPLAPVLLAYMGRFQLGE
                     VQFLMALAFIPWIAIRYLAYRGWQLSPFVGLFLGAV"
BASE COUNT          359 a          398 c          410 g          496 t
ORIGIN      
        1 aaaggcaggc aaaatcataa aaagatagcc gtagactaat ggcgttctta tgcaatgtaa
       61 aagcttaccg gccttagtcc tcagtgactt cattgattta accaagttac ccctgcagtt
      121 gattaagctt tctgacgggc aagactgtca aaatttctaa gggaacaact ctcaattatt
      181 gtgctgattc cagtggcagt caagcgacta gcgcatttga ctgccactgg tttagacttt
      241 ggtatcctct ttttgtgagg catctcagct ctaaccgtta gatctttaga ctggttacaa
      301 agagggggcg cggggcatgg tcgcactatt tcgtccggaa tcgctcaacc gttctggtcc
      361 cgtgcaggaa gccaatattt ctatgaattt gcttctactt tttttgaagc caggatagtt
      421 tcagaacctc agccgataga aggctgaaag ctagagggag tgctccttcc taaagcattc
      481 tcaaacgaaa aatatacgtt gatcatgaca caccatagct gaagctttct gaattttggt
      541 gtatacttca gctgtcggct tggggccctt gcagagtgct ctttaggcat tttagatagt
      601 tgcattagcg atttttcgtc gaaccagact cggtcccatc ccgtaatatc gcttcagtgt
      661 tggcatttgc ctttctccgg cgggattgat ttttcccgcc agacaaagcg attgaggtta
      721 ggtagtttct aggcagatgg tcgggaattg gttatggaca atcgttagac aaaaccaatg
      781 aatctatcgg gcgctttcgc acgcgaaatg catactgcgc atctgtcctg gaaaaatgcc
      841 cattccaact gcttctttcc acaagacatt cttcgttgac gtaaaatcac atgctcaaga
      901 atgttttgct cgagggcgac taataaaata gccttatggg tggtctcttt gcaactctgc
      961 gaaccagctc aacgccctaa ttttaaaatg tataatagtc gtcattttta tgaggctaaa
     1021 gctcttgcct gtgttgtcag ctaaatcttt caaggtgcgc tttcgggctc tctttttgac
     1081 atcagaggca gcggcttttt acttgctgac cttgcctgcc ttctggctta tcgggcgcga
     1141 ttatttgacg cgccgcggcg atctttcgcc catggtggcc atgcatcttg tcatcaacca
     1201 gcttgtgacc accgagcagg tgccctatgt cgattttctc gacctggcgc agccgataat
     1261 ttatgagtta taccgattgc cggccttgct caacgatgcg cttggttttt gtggtatgcc
     1321 gatgcgtctg gaagaagtcg tcaagctcat ttttattgtt ctcaccgcac tatcatctgt
     1381 tgcctgcttc ttgattctag ggctggccag gagcagtttg caggctcggg tgggcaccga
     1441 gcctgctgag aaggcggaag aagaggctct gactgaagcg ctcagcctgt cccaggtggc
     1501 tttcccgctg gcgccggtct tgctagctta tatggggcgc tttcaactgg gcgaggtgca
     1561 attcctcatg gcgcttgcct tcattccctg gattgccatt cgctatctgg cgtatcgggg
     1621 gtggcagttg tcgccctttg tgggcctctt tctcggcgct gtt
//
LOCUS       DAOQNZ010001039         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_55603_length_1662_cov_3.573740, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001039 DAOQNZ010000000
VERSION     DAOQNZ010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55603_length_1662_cov_3.573740"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PKW73_16125"
     CDS             <1..480
                     /locus_tag="PKW73_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNA74878.1"
                     /translation="AFIEELEATCTRGLVPVLGAHQVVAALAAHQFAVITSGSNRLAS
                     HRLTHAQLPIPRVFITADDVINGKPHPEGYLKAAKELGIKPEDCIVLEDSPAGIRAGQ
                     AAGMRTIAIAAHSGNHDISHSDFVIQDLTWLKVDSDKSNDEELTIFIKPLRQSGTSQ"
     gene            complement(584..>1662)
                     /locus_tag="PKW73_16130"
     CDS             complement(584..>1662)
                     /locus_tag="PKW73_16130"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNA74879.1"
                     /translation="LSLNFFLGTMPLFLYLQAPLERFRKILLFFSICICLVVYGLHAD
                     WHNNCHKASEFEAPPEVAGLRDELAREHVRIFSIKGLEGGSHELPANICRLWGIANAS
                     GYSPLVPWPYARLMGIAEGGFLKPPWVLRAENRAFDIASVKYVLAPEVEVPPQFVDSE
                     NRDNWTMRKSESGVIYFENRRVMPRFRLVDEAISLEMRDALRSCRTSLLPGGRVFHPE
                     RSVIITTGEASLPLEKNTRITGRSIIAELLAPYKPAAGTVQLLFESESSIDLQVEAIR
                     PSYLVIADQYYPGWQATIDGIPVAIQRANCAFRAIAMPSGTHRVSMKFRSQSVSQGMY
                     LSLVGLFLLCVFVWSYSRKTSKLR"
BASE COUNT          456 a          444 c          405 g          357 t
ORIGIN      
        1 gcttttatcg aggagttgga agcgacttgc acacggggac tggtgccggt gctcggtgcg
       61 caccaggtag tggcagcatt ggcagcccac caatttgcag taataacttc cggctcaaat
      121 aggctggcca gccaccgcct cacccatgcg cagctcccta ttccgagagt tttcatcaca
      181 gccgatgatg taattaatgg caaaccgcat cctgaaggct atctcaaggc agcaaaagaa
      241 cttggcatca agccggaaga ctgtattgtt ctggaagatt caccggcagg gataagggcg
      301 ggtcaggcag caggcatgag aacaattgcc atcgctgcac actccggcaa ccatgacata
      361 tcgcatagcg actttgtaat tcaggacctg acctggttga aagtcgatag tgataaatcc
      421 aatgacgaag aacttacgat tttcataaag ccgctgcgcc agagtgggac ttcccagtaa
      481 tttcgaagtt tttctcgaac cgaaccctac cgaattactg aagtttttct tgtttaacac
      541 agtcgataaa atcagatgat tttctcgaat tccatattag tgcttagcgc aatttcgaag
      601 tttttctcga atagctccaa acaaatacac agagcaagaa aagcccaacc aggctcaaat
      661 acatgccctg gctgacagac tgggatctaa atttcattga tacccggtgc gttccagagg
      721 gcatggcaat tgcccggaat gcgcaattgg cccgctgaat cgccacagga ataccatcta
      781 ttgttgcctg ccaaccaggg tagtactgat ctgctatgac caggtaagaa ggtcgaatcg
      841 cttccacctg caaatcaata ctgctttccg actcaaaaag tagttggaca gttccggcag
      901 ccggtttata aggcgcgagc aattcagcaa ttatagaacg cccggtgatg cgcgtattct
      961 tctctagcgg gagcgaagct tcaccagtgg tgatgatgac gcttcgctcc gggtgaaaga
     1021 ccctgccgcc cggcaaaagc gatgtgcggc atgagcgcaa ggcatctctc atctccaggc
     1081 tgatagcctc gtcaactagc cggaagcggg gcatgacccg acgattctca aagtaaatga
     1141 caccggactc tgactttctc atcgtccagt tatcgcgatt ctcagagtcg acgaactggg
     1201 gcgggacctc cacttcaggc gccagaacgt acttcaccga ggcaatgtca aaagctctat
     1261 tctcagcacg caagacccat ggcggcttca ggaagccacc ttcggcaata cccatcagcc
     1321 gagcataagg ccacggtacc aggggagaat agccggaagc attggctata ccccagagac
     1381 gacaaatgtt agcagggagt tcatggctac cgccttccag cccctttatg ctaaaaattc
     1441 gtacatgctc ccgggccagt tcatcgcgca gaccggcaac ttccggcggc gcctcgaatt
     1501 cggaagcctt gtgacagttg ttgtgccaat ccgcatgaag cccatacaca acaagacaga
     1561 tacagatcga gaaaaacagc aaaattttcc gaaaccgctc aagcggagcc tgcagataca
     1621 aaaacaacgg catggttccg aggaaaaagt ttagactcaa cc
//
LOCUS       DAOQNZ010001040         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_55882_length_1658_cov_3.247661, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001040 DAOQNZ010000000
VERSION     DAOQNZ010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55882_length_1658_cov_3.247661"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PKW73_16135"
     CDS             complement(<1..967)
                     /locus_tag="PKW73_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74880.1"
                     /translation="MKAEKRRQILLILAIFLLLAGLLFWQQLLLASPLFFGYRGPDMF
                     GIYTADLDGKNFHPLLLDARREMTHARISPDGKKITFTRYNPSKRGILPSDYAEENGS
                     NYLNTEIVVADLSGSAHNRTLACSNQKDVEPWGEEIMNANSSWIDNDTIIYIHRGNLT
                     SLPQLKLYHLADHRSEVVNTPSGLAAADPHCINGQLIFPVVPLSQDKNAVCRLYTMKL
                     DGTQVRQLPQGQIVSTSDLRAFKLGDYDPWLAPDGKTAVFMRHFGGEDWRVFKIDLVK
                     GEEKQLTATGITSGIPKWSPDGKEIIYVSWDKSKLENLGLNTMGAT"
     gene            complement(957..>1658)
                     /locus_tag="PKW73_16140"
     CDS             complement(957..>1658)
                     /locus_tag="PKW73_16140"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNA74881.1"
                     /translation="EVWAALASLTSMEIILGIDNIIFISILSSMLPAKQQNTARIAGL
                     ALAMLSRIALLASLSWVMSLKEPLFSLASHSFSGRDLLLLAGGAFLVAKATKEIHGRI
                     EGEAETADKKASPSLWGTLIQIAILDMVFSLDSVITAVGMVDQIWIMIVAVIIAVIIM
                     MIFSQPVSQFIHKFPTVKMLALSFLLLIGVVLAAEGCGQHINKAYIYAAMGFSIFVEA
                     LNLAASARRKKTIES"
BASE COUNT          400 a          396 c          449 g          413 t
ORIGIN      
        1 ctgtggcgcc catggtattg agacccaggt tttccagctt cgacttgtcc caggagacgt
       61 aaataatttc tttgccgtcc ggcgaccatt tgggaatgcc gctggtgata ccggtggcgg
      121 tgagttgttt ctcttcgccc ttgaccagat cgattttgaa aacacgccag tcttcaccgc
      181 cgaaatggcg catgaaaact gctgttttac catccggtgc cagccaggga tcgtagtcac
      241 ccagcttaaa ggccctcagg tcggaagtgc tgacaatttg accttgcggc aactgcctca
      301 cttgcgtgcc gtcgagcttc atcgtataaa gcctgcaaac ggcatttttg tcctgggaga
      361 gtggtaccac cggaaaaatc agttgcccgt tgatgcagtg gggatcggcc gctgccaggc
      421 cggaaggcgt gttcaccact tcgctccggt ggtcggccag atgatagagt ttgagctggg
      481 gcagggaagt gaggttgccc cggtgaatgt agataatggt gtcattgtct atccatgaac
      541 tattggcatt cataatttcc tcaccccagg gctccacatc tttttgattg ctgcaggcga
      601 gggtacggtt atgggccgag ccgctcaggt cggctacaac tatttcggta tttagataat
      661 tgctgccgtt ttcttcggca tagtcggagg gcagaatgcc tcttttgctc gggttatagc
      721 gggtaaaggt gattttctta ccatccgggg aaattcgggc atgggtcatt tctctccttg
      781 catcaagcag aagggggtga aagttcttcc catccaggtc ggcagtataa atgccgaaca
      841 tatcgggacc ccggtagccg aaaaagagag gactggcaag caacagttgt tgccagaaga
      901 gaagaccggc cagtaagaga aagatggcca aaatcaagag aatctgtctt cgcttctcag
      961 ctttcaatgg tcttcttcct tctggcactg gcagctagat tgagagcttc tacgaatatg
     1021 gaaaagccca tggctgcata gatataggct ttgtttatgt gctgtccgca gccttcggcg
     1081 gctagtacca cacctattag caggaggaag ctcaaggcaa gcattttaac cgtgggaaac
     1141 ttgtgaatga attgactgac cggctgagag aagatcatca tgattatgac ggcgatgatt
     1201 acggctacta tcattatcca aatctgatca accatgccca cagcggttat aactgaatcc
     1261 agggaaaaga ccatgtctag aatggcgatt tgaattagtg tgccccacag ggatggactt
     1321 gccttcttgt ctgcagtttc ggcttcacct tcgattcttc cgtgtatttc tttggtagcc
     1381 ttcgccacca gaaaggcacc accggccagc aagagcaggt ccctgcctga gaaggaatga
     1441 gaagccaggc taaagagcgg ctctttgaga ctcatcaccc aactcaaaga agcaagcagg
     1501 gcaattcttg agagcatggc cagagccaga ccggcaatac gggctgtgtt ctgttgcttt
     1561 gccggaagca tggaagacag aatcgatatg aagataatat tgtcgatgcc aaggattatt
     1621 tccatggagg tgagagaggc cagtgccgcc cagacttc
//
LOCUS       DAOQNZ010001041         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_56003_length_1656_cov_3.721424, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001041 DAOQNZ010000000
VERSION     DAOQNZ010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56003_length_1656_cov_3.721424"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PKW73_16145"
     CDS             complement(<1..786)
                     /locus_tag="PKW73_16145"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74882.1"
                     /translation="MKLFFYLHTHWDREWYWNFNAYRLELTTVVKRALEVVRNGECRG
                     FMLDGQTSLIDDVLEVSPSLQEEIVAANVAGKLFIGPWFVLADQLLVGGESLVRNLAL
                     GLSRAQELGGSARVGYCPDTFGHSLDLPRILRGFGIDNAVVWRGVPALNEPLFQWRSP
                     DGSSVLALQMNAGYYHTAFFDGVSDEQMEAYLLAFLGRQRGKNGIEPVSFAPGLPGKK
                     GSYIEALAGALVPVGGDHIMPPVNFSGQLQKLRPRLQELDVELQ"
     gene            complement(822..>1656)
                     /locus_tag="PKW73_16150"
     CDS             complement(822..>1656)
                     /locus_tag="PKW73_16150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNA74883.1"
                     /translation="GAVELAAHHAIASVEYSFAPFVVKTGKGAKSQDDKEAAYFASVA
                     ENARKLGVEFSILNLDYALDPLDKRAVKQFSPMLQKLAVVARNLSCPRLGFSLLPAAD
                     EDFLAAFLTQYAELKALEELSTLELVMRLAAPPSMRGQSLRKWRPLEPGEWRELLAGC
                     EGLSFSYSPADCLWQGIDYLQNIGHFAQAISHVVAHDMEINRTILGDSGMYGPLWWRY
                     RVAGKGQVDFRQVIELLKLYDFQGALSMQFEDEFLSDDPLQLSESLEEGVKYFAPLMK
                     G"
BASE COUNT          419 a          511 c          412 g          314 t
ORIGIN      
        1 ctgcaattcc acatccagct cttgcagacg cggtctcaat ttttgcaatt gcccggaaaa
       61 attcacaggc ggcatgatgt gatctccgcc gaccggcacc agcgccccag ccaaagcttc
      121 aatgtaggag cccttcttac ctggtaggcc gggtgcaaag gatacaggct cgatgccgtt
      181 cttgcctctc tgccgaccga gaaaagccag caaataagct tccatctgtt catcgctcac
      241 accatcaaaa aaagccgtgt gatagtagcc ggcattcatc tgcaaggcta aaaccgatga
      301 accgtcaggg ctgcgccact ggaagagagg ctcattcagg gccggcacgc cccgccagac
      361 cacggcattg tcgataccga aaccgcgcag aatgcggggt aaatccagag aatgcccgaa
      421 ggtatccggg caataaccca cccgggccga gccccccagt tcctgggcgc gactcaagcc
      481 cagagccaga ttgcgcacca gactctctcc accgacaagc agttgatcgg ctagcacaaa
      541 ccagggaccg atgaaaagct taccggccac attggctgcc acgatttcct cttgcaggga
      601 gggcgagacc tccagtacgt cgtcgatcaa ggaagtctgt ccgtccagca taaagccgcg
      661 acactcgccg tttctcacca cttccagggc ccgcttcacc accgtcgtca attccagacg
      721 ataggcattg aagttccagt accactctct atcccagtgt gtatgcagat agaagaagag
      781 cttcatggca ggaatccttc caacaaggca gctaaaaaac gtcagccctt cataagaggg
      841 gcgaaatatt ttacgccctc ttccaggctc tcggataatt gcagcgggtc atcgctgagg
      901 aattcatcct cgaactgcat tgagagggcc ccctggaaat cgtagagctt gagcagttca
      961 attacctgcc ggaaatctac ctgccctttg ccggcgacac gatagcgcca ccagagcgga
     1021 ccatacatac cactgtcgcc aagaatggtg cggttgatct ccatatcgtg cgccaccaca
     1081 tgcgatatcg cctgtgcaaa atgcccgata ttctgcaaat aatcaatacc ctgccagaga
     1141 cagtctgccg gcgagtagga aaaggaaagc ccttcgcagc cggccaacag ttcccgccat
     1201 tctcccggct caagcggtcg ccacttgcgc agcgactgcc cgcgcatgga aggaggtgcg
     1261 gcaaggcgca ttaccagctc cagagtagaa agttcttcca gcgcttttag ctcagcatac
     1321 tgagtcagaa aagcagccag gaaatcttcg tcggctgccg gtaaaaggga aaagcccaga
     1381 cggggacaag acagattccg cgctaccacc gccagctttt gcaacatggg cgagaactgc
     1441 ttgaccgccc gcttgtccag cggatccagg gcatagtcaa gattcaagat agaaaactct
     1501 accccaagct tcctggcgtt ctccgccaca gaagcgaaat aagccgcctc cttgtcatcc
     1561 tggcttttag ctcccttgcc ggtcttcacc acgaagggcg cgaaagaata ttcaacggag
     1621 gcaatagcat ggtgcgccgc aagctctacc gccccg
//
LOCUS       DAOQNZ010001042         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_56148_length_1654_cov_3.347092, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001042 DAOQNZ010000000
VERSION     DAOQNZ010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56148_length_1654_cov_3.347092"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PKW73_16155"
     CDS             complement(<1..1237)
                     /locus_tag="PKW73_16155"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA74884.1"
                     /translation="MTGREELPDLGKEGFINSSLSFLTFSYRENLYGQLDKLVENGKS
                     LDGLIIDLMQTGFSLPLDALVKFAIYGKKLLKPHGVLLFVKGDGAITLTRDDPAGGLT
                     FNFYDSPFGIFTRYPLLADYACATVSGIDRRRLRGGGSTLANHILSTSIPVMSDYGKS
                     VKNDFTLPAPQNWVMQMIDDYASVESIVRNVEARHQLPSDEALRILQEFEAARLIYPV
                     FSRIQFLSNCYHNRKPFRLGRYLVASGIIDERQLRELLEKQQEEGWGKSQRSFLGLLC
                     VRAGFINTRELEVLLDDQYLYGGYHKRTEDDAAGNSRSVNIETMRDSMIGSLGAIDTA
                     GLLQSLATAKKTGLLTVDNRDKTIIVAFESGRPTHARLNKLRGYEAMVEFITTWAEGI
                     FVFRDKGRSEELDDTARLSQ"
     gene            complement(1272..>1654)
                     /locus_tag="PKW73_16160"
     CDS             complement(1272..>1654)
                     /locus_tag="PKW73_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74885.1"
                     /translation="RPEIAKSWLIDPKLISIPKYMGLIGRSTPPLRLVPMSGPAGGGA
                     RFRLVTSSKDDLIIEVGRILSGKLKGQVAIKALFVAPPDPIAQSLMGILVVPHAAAPT
                     GGTSQTVHAPAAAGKASPKPGAPR"
BASE COUNT          411 a          448 c          437 g          358 t
ORIGIN      
        1 actgactgag tcgggctgta tcatccagtt cttccgaacg acctttgtct cgaaagacga
       61 aaataccttc agcccaggtg gtgatgaact ctaccatggc ctcatagcca cgcagtttat
      121 tgagacgagc atgggtcggt cgtccagact cgaaggccac aataatggtc ttgtctcgat
      181 tgtccactgt cagcagacct gttttcttag ctgtggccag agactgaagt aggccggcag
      241 tgtctatggc acccagacta ccgatcatgg aatcacgcat ggtctcgata ttgacggatc
      301 tggaattacc ggcggcatca tcctcggtgc gtttgtgata gccgccatag agatactgat
      361 catccagcaa tacttctaat tcgcgagtgt tgatgaaacc cgcccgcacg cataaaagcc
      421 ccagaaaact acgctgcgac ttgccccagc cctcttcttg ctgtttctcc agaagctcac
      481 gcaattgtct ttcgtcaatg ataccgctgg ccacaagata gcgacccaag cgaaagggct
      541 tgcgattgtg gtagcaatta gatagaaact gaatgcggga aaaaaccgga tagattagac
      601 gagccgcctc gaattcctgc agaatccgca acgcttcatc agaaggcagt tggtgtctag
      661 cttccacatt tcgaacgata gattccaccg aggcatagtc gtcgatcatc tgcatcaccc
      721 agttttgagg agcaggcagg gtaaagtcat ttttaacaga cttaccgtaa tcggacataa
      781 ccggaataga agtagacaat atgtggttgg cgagggtgct tccgccaccg cgcaagcgcc
      841 gccgatcgat accggatacg gtggcacaag cataatcggc aagcagcgga tagcgcgtga
      901 agattccgaa tggactatcg tagaagttga aggtaagccc cccggccgga tcatctcggg
      961 tcagagttat ggccccgtct cctttgacaa aaagtaagac cccatgaggc ttaagcaatt
     1021 tcttgccata gatggcaaat ttgaccagag catccagagg cagcgaaaac ccggtctgca
     1081 tcaaatcgat gatcagacca tccaggctct tgccgttttc cactagcttg tcgagctggc
     1141 cgtagaggtt ctcccggtaa gagaaggtga gaaagctcaa agacgagttg ataaaccctt
     1201 ctttgcccag atcgggaagc tcttcccgcc cagtcaaaac taatccctca ccacttcagc
     1261 ctctcgcaat atcatcgggg cgctcccggc ttggggctgg ctttaccggc cgctgccgga
     1321 gcgtgtacgg tctggctggt accaccagtg ggagccgccg catggggcac aaccaggata
     1381 cccatcaaac tctgggcgat gggatcgggc ggagcaacaa aaagagcctt gatagccacc
     1441 tgtcccttta acttgccgct cagaatccga cccacttcga tgatcaaatc atccttgctg
     1501 gaagtaacca ggcggaagcg cgccccgcct ccggccggac cggacatggg taccaggcgc
     1561 aggggcgggg tgctgcggcc aatcaagccc atgtacttgg gaatggagat gagtttaggg
     1621 tcgatgagcc aacttttggc gatttccgga cggg
//
LOCUS       DAOQNZ010001043         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_56149_length_1654_cov_3.308318, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001043 DAOQNZ010000000
VERSION     DAOQNZ010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56149_length_1654_cov_3.308318"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..464)
                     /locus_tag="PKW73_16165"
     CDS             complement(279..464)
                     /locus_tag="PKW73_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74886.1"
                     /translation="MDLLEKILLFIIGLTFVGGGGILTKQAFEDAKNVFECIIFGMLV
                     ASVGVLLCVWTFTGPPD"
     gene            complement(548..859)
                     /locus_tag="PKW73_16170"
     CDS             complement(548..859)
                     /locus_tag="PKW73_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74887.1"
                     /translation="MVSRKVYSFLLCLVLPLWFGGLPVEASDRDKDKSLLSNALLVDG
                     MTRRRKSIMRTYTPLTQYKPMSSVSREERFVQMLLTSLGIVGIFLAILALRSMPPPAK
                     S"
     gene            complement(909..>1654)
                     /gene="amrS"
                     /locus_tag="PKW73_16175"
     CDS             complement(909..>1654)
                     /gene="amrS"
                     /locus_tag="PKW73_16175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNA74888.1"
                     /translation="AMPEQIAAAAREFDCAAVAFTYNDPVIWAEYAIDTAIACHEVGV
                     KTIAVTAGYINPGAREDFYAHIDAANVDLKAFSQHFYSRLTLSNLAPVLDTLIWLKTE
                     SKVWFEVTTLLIPEENDSDRELEAMCAWFVENLGADVPLHFSAFHPDFRLVNRPATPA
                     ATLVRARDIALKHGLKYVYVGNVDDSRRQATYCPACKKVLIERNWYDLGGYHIKDGAC
                     KYCREPIAGVWPVKKGTWGRRRQPVNIVG"
BASE COUNT          401 a          464 c          438 g          351 t
ORIGIN      
        1 ccttctccag attggtctta ggcttggctt ccaccggggt tgagaccagg gtgacattca
       61 cagccatagc taaggtcagc gccatcaaaa ccgacgccgg tacagccctt ttgatcaggc
      121 tggctgcctt ccccgggatt accttggctt ttgaagacaa aatgcttgcg cccatgacac
      181 tctttacctc tcatgctaca actgcggcaa attgagagtt tactacgtct gaaagcgcgg
      241 aaacttagca ggatttcagt cgaaagcccg gtcagcaatc aatccggcgg accggtgaaa
      301 gtccagacgc agagcaaaac ccctacggaa gccaccagca tgccgaaaat gatgcactcg
      361 aagacgtttt tggcgtcctc gaaagcctgc ttggtcaaga ttccgccgcc cccgacaaaa
      421 gttagaccga ttataaatag cagtattttc tctaataggt ccatttacga gccttcgaag
      481 gtagaggaca gtgctcacgc tcacggggct taacttcctc agccctggag ggctcgattg
      541 aggacaacta agatttagca ggcggcggca tagaccgcaa agcaagtatt gctagaaata
      601 ttcccacaat tcccagagaa gtcaaaagca tttgtacgaa gcgctcctct ctgctcaccg
      661 aggacatcgg tttatactgg gttaacggcg tataagtgcg cataatcgac ttgcgccggc
      721 gggtcatgcc gtccaccagc agggcattgg aaagcaggct cttatccttg tcgcggtccg
      781 aggcctcgac gggcaggccc ccgaaccaga ggggaagaac caggcagaga aggaaagaat
      841 agactttacg ggataccata aaccggaaca agcctttgct gtttgttcga aaaccgccgc
      901 ctcgcgggtc accccactat attaacaggc tgccgccgcc gcccccaggt gcctttcttg
      961 accggccaga ccccggcaat gggttcccgg caatacttac aggcaccgtc ctttatatga
     1021 tagccgccca aatcgtacca gttgcgctca atgagaacct ttttgcaagc cgggcagtag
     1081 gttgcctggc gccgggaatc gtctacattg cccacataaa catactttag cccatgcttg
     1141 agagcaatat ctcgggctct taccagggtg gcagccggtg tggcgggacg atttaccagg
     1201 cggaaatcag gatgaaaggc ggaaaagtgc aggggtacat ccgcccccag gttctcgaca
     1261 aaccaggcgc acatagcctc cagttctctg tcgctgtcat tttcctcggg tatgagcagg
     1321 gtagtaactt caaaccagac cttgctttcc gtcttcagcc agatgagggt gtccagcaca
     1381 ggcgccaggt tggaaagggt cagccgggaa tagaaatgct gtgaaaatgc cttcaaatcc
     1441 acgttagccg catcaatatg ggcatagaag tcttcccggg cgcccggatt tatgtacccg
     1501 gcagtgacgg cgatggtctt cacacccacc tcgtggcagg cgattgccgt atctatggca
     1561 tattcggccc agatcaccgg gtcgttatag gtgaaggcta cggcagcaca gtcgaattcc
     1621 ctggccgccg ccgcaatctg ctcgggcatg gcgg
//
LOCUS       DAOQNZ010001044         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_56229_length_1653_cov_3.270964, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001044 DAOQNZ010000000
VERSION     DAOQNZ010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56229_length_1653_cov_3.270964"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..998
                     /locus_tag="PKW73_16180"
     CDS             189..998
                     /locus_tag="PKW73_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNA74889.1"
                     /translation="MPEFNRAENPGDNESRKAEELKESFTLSLTNEEGMLLLTSARLL
                     DKLVAGVTIHAQERDQLIKEIIAKLDKKVPEDVSRVAAELADAIVDSVICEGELEDDY
                     GFLSDSIPMGIGDEDFRALRRDIAEEIMNDERLAESPELLQRDLGGPSGFSVQKFARD
                     LDFDQTVFDYPMYNTDKTLPVLEQALINHHSVKAHYYSILRETVDTVTLDPLVILKEH
                     SLWRMVAYCHERRDILVFRVDRIKDIGETKTSFKAPADVSNLSYSRLPAYS"
     gene            1091..1594
                     /gene="rpiB"
                     /locus_tag="PKW73_16185"
     CDS             1091..1594
                     /gene="rpiB"
                     /locus_tag="PKW73_16185"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNA74890.1"
                     /translation="MTDNKSPSPDANETGRVVAIGSDHAGFELKQAVAQVLEKMGYQV
                     VDFGTNSKDSCDYPDYARQVATAVSAGQAGRGIICCGSGIGVSIVANKVRRVRAALVH
                     DAEQASLSRQHNDANVLCLAGRSTPVESLAGIIDTWMTTAFEGGRHERRVKKIEVSRA
                     FEQGLFS"
BASE COUNT          392 a          395 c          464 g          402 t
ORIGIN      
        1 gctgggtctt attttggcca caaagcaggt actttccaat gctctgggca taataggggt
       61 atcggcgccg gagcgaatgt aagagaaaga cctggaactt attccgcgtc gtaagtagtc
      121 cggcaccttt ccggtcttgg caaccagtgc taggctagag atatgtcctt tacgtcgagg
      181 agagttttat gcctgaattt aacagggcag aaaatcccgg ggataacgag agcaggaagg
      241 ctgaggaact caaggagagt tttaccctca gtctcaccaa cgaagagggc atgctccttc
      301 ttaccagtgc caggctcctg gataagctcg tggccggtgt caccatccat gcccaggagc
      361 gtgaccagct cataaaggaa atcattgcta aactggacaa gaaagtgccg gaagatgttt
      421 ccagggttgc cgctgagctg gccgacgcca tagttgactc ggtgatttgc gaaggtgagc
      481 tggaagatga ttacggcttc ctctccgatt ctattccgat gggaatcggc gacgaagatt
      541 ttcgcgcctt gcggcgtgac atagccgaag agattatgaa cgatgagcga ctggcggaga
      601 gccccgagtt gcttcagcgg gatttaggcg gcccctcagg tttctccgta cagaaattcg
      661 ctcgcgatct ggatttcgat caaactgttt ttgactatcc tatgtataac accgataaaa
      721 cattaccggt gttggagcag gcgctaatca atcatcacag cgtcaaggca cattattaca
      781 gcattttgag agaaacggtg gatacggtta ccctggatcc attagtgatt ttgaaagagc
      841 atagcctctg gcgaatggtg gcttattgcc atgaacgccg cgatatattg gtatttcgcg
      901 ttgatagaat caaagatatc ggcgagacga agaccagttt caaagccccc gccgatgtct
      961 caaacctgag ttacagtcgt ctgcctgctt attcctgaag cttacagaaa atctatcctc
     1021 tcatgacctt gccgtcgcgc tattttgcta tgctcagttt gaggaattga ggagcgcaag
     1081 aggaaggttc atgacagata acaaaagccc gtcgccggat gcaaacgaaa ccggcagggt
     1141 cgtggccata ggcagcgatc acgccggatt tgagttgaag caggctgttg ctcaggttct
     1201 ggagaagatg ggctatcagg ttgttgattt tggtaccaat tccaaagatt cttgtgatta
     1261 tccggattat gccagacagg tggccactgc cgtttctgcc gggcaggccg ggcgcggcat
     1321 tatttgctgc ggctccggca tcggtgtttc tattgtggcc aataaagtga gacgggtgcg
     1381 tgctgctctc gttcatgatg ccgaacaggc atcactatcc cggcagcata atgatgccaa
     1441 tgtgctctgt ctggccgggc gctccactcc tgtagagagt cttgccggca ttatcgatac
     1501 ctggatgact accgcctttg agggcggccg gcacgagcgt cgcgttaaga aaatcgaagt
     1561 gagccgcgcc tttgaacagg gtctcttcag ctagtgcgga tagaccgttg agcggcttcc
     1621 tctctgcgcc agacggcgaa acattgcggt agc
//
LOCUS       DAOQNZ010001045         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_56440_length_1650_cov_2.964890, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001045 DAOQNZ010000000
VERSION     DAOQNZ010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56440_length_1650_cov_2.964890"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKW73_16190"
     CDS             complement(<1..273)
                     /locus_tag="PKW73_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74891.1"
                     /translation="MVTAPNLERSEESNEAQPVRILAPGAAFKEAARKKANTGPSGKG
                     KEGNPGQRPRRKRPLPPGQRLVRAFAVASLLVAGQSFFSPLTGPCEN"
     gene            complement(536..1069)
                     /locus_tag="PKW73_16195"
     CDS             complement(536..1069)
                     /locus_tag="PKW73_16195"
                     /inference="COORDINATES: protein motif:HMM:NF025390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
                     /protein_id="HNA74892.1"
                     /translation="MDELTFWYLIDQSIDLTEELLADKQGLDPIDAQLSTLKKLLSKL
                     SEDELVGFQEIVDYLMDQAYTWSLWGAAYLMMGGLSDDDFEYFRAGLILRGKEVYEGA
                     LAEPDSLAEHTEIQLCEDLLFLACEVYEERSGDGAIYDRYREVMNPEPAGEPFDEDDE
                     GYFREHYPRLWQLYSQE"
     gene            complement(1088..1579)
                     /locus_tag="PKW73_16200"
     CDS             complement(1088..1579)
                     /locus_tag="PKW73_16200"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNA74893.1"
                     /translation="MATNNIKVSIMAFALGITFGLGTIWILVMNGMLIGTVFGVCQDY
                     GMAGRLAAFVAGHGVIELTAIFISGGAGLMLGAAMLFPGQFKRVDSLRRAVRPAMEMF
                     AGCVLMLLVAGTIEGFVSPRTDIGADVKYMVSAATAAILMLYLFIPRPKAHHLVSELP
                     ETK"
BASE COUNT          424 a          461 c          411 g          354 t
ORIGIN      
        1 gttctcacag ggtccggtca ggggagagaa gaagctctgt ccggccacaa gcaaagaagc
       61 cacggcgaaa gcacgcacca ggcgctggcc gggcggcagc ggccgtttgc gcctgggtct
      121 ttgaccagga ttgccctctt tgcccttgcc actagggccg gtattagcct tcttgcgggc
      181 agcctccttg aaggcggcgc cgggagccaa aattctcaca ggttgagcct cattcgactc
      241 ttccgaccgc tctagatttg gcgctgttac cattgagccg ggttccttac tagaaaatca
      301 aaaaaaccat acatgaggaa agcaaaacga agcaaaagat atctatcgca aataaagatc
      361 cgttacagtc gtcatctttc cggggcaagc actggatcat cgtatgccgc tatgggaaaa
      421 gatgatacca gggacgctac cgctatttta gctcagatgg ctaacaagtc cagtagcaat
      481 tttgccgtac cgccaattaa tttggccctg aagctgccgg aaaagcgccg ccctctcact
      541 cctggctata caactgccag agacgaggat agtgctcgcg aaaatagcct tcatcgtctt
      601 catcaaaagg ctccccggcc ggctcgggat tcatgacctc gcggtagcga tcataaatgg
      661 caccatcacc actgcgctct tcatatacct cacaagccag gaagagcaag tcttcgcaaa
      721 gctgaatctc ggtatgctca gccagggagt cgggctcagc cagtgctcct tcatatactt
      781 ccttgccgcg caagatcaag ccggcccgaa agtactcgaa gtcatcgtcg gacagaccgc
      841 ccatcattag ataagccgcc ccccatagac tccaggtata agcttggtcc atcaagtaat
      901 cgacaatttc ttgaaagccc acaagctcat cttcgctcaa cttggacaac aatttcttga
      961 gtgtcgaaag ttgagcgtcg ataggatcta gaccctgctt atcggctaaa agttcctcgg
     1021 ttaaatcaat tgactggtct atcaagtacc agaatgtcaa ttcgtccatt ttcaggggtt
     1081 gcctttctta cttggtttcg ggaagctcgg agacgagatg gtgagctttc ggacgcggaa
     1141 taaaaagata aagcatcagt atggctgccg tagcagcgct gaccatatat tttacatcgg
     1201 caccgatgtc ggtgcgcggc gaaacaaagc cttctatggt accagcaacc aatagcatca
     1261 gtacacagcc ggcgaacatt tccatggccg gccggacggc ccggcgcagg gaatccaccc
     1321 gcttgaactg cccggggaag agcatggccg cgcccagcat caaaccggcg ccgccactga
     1381 taaagatggc ggtcaactct atgacgccgt gaccggccac gaaagcagcc agtcgcccgg
     1441 ccatgccgta gtcctggcag acaccaaata cagtgccgat taacatgccg ttcataacca
     1501 atatccagat cgttcccagc ccgaaggtaa tacccagggc aaaggccatg attgaaactt
     1561 tgatattgtt ggttgcaatg gcgctggaga caatatcaaa gtttcaatca tggcctttgc
     1621 cctgggtatt accttcgggc aaaggccatg
//
LOCUS       DAOQNZ010001046         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_56487_length_1649_cov_4.849435, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001046 DAOQNZ010000000
VERSION     DAOQNZ010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56487_length_1649_cov_4.849435"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKW73_16205"
     CDS             <1..401
                     /locus_tag="PKW73_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74894.1"
                     /translation="NMTITLLLQWLLAGFFWHVPEDAIGIALFIVGACLLAEHLAKRK
                     EQMPAIIAVESLEAELEFIDGMMQSHLPSYDKDKAWLVERNLKIAREQLRLAGESIWK
                     ERWSDCLECAEHGMVQVCLLRRTLGIRHEL"
     gene            complement(792..1190)
                     /locus_tag="PKW73_16210"
     CDS             complement(792..1190)
                     /locus_tag="PKW73_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74895.1"
                     /translation="MKANEPIDTLKIRQPQSGRLEVLLNNPGVYFELVGKDFVTGFGL
                     GVGQRDWQRKVELLAAVIATLCGAKSDDVASLDGLQVKVKWKSGTQIEALGWIDAPEA
                     IPEAVAPFIDAEGWFYPELFGQTYDPLGVV"
     gene            complement(1277..>1649)
                     /locus_tag="PKW73_16215"
     CDS             complement(1277..>1649)
                     /locus_tag="PKW73_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74896.1"
                     /translation="YPIETLMNNENAFLTLFAAFMVSIAVCLGIAWAGPVTIWIAGLS
                     VFCLVLWTGWSSAGINNIAGVNILNLSGIWSWLGLTVWFGIASIPHWPWFAFVVLFLA
                     FSVRWLLKKSRCSCPELPEAD"
BASE COUNT          419 a          449 c          405 g          376 t
ORIGIN      
        1 cgaatatgac aatcacactt ttactacagt ggctactggc cggcttcttc tggcatgtac
       61 cagaggacgc tatcggcatc gcgctcttca ttgtcggagc ctgcctcctt gccgagcacc
      121 ttgccaaacg caaagagcag atgccggcga taattgcagt tgaatcactg gaggcagagc
      181 tagagtttat cgacggcatg atgcaatcgc atctgccctc atatgacaaa gacaaagcct
      241 ggctggtcga acgaaacctg aaaattgcca gggagcaact tcgcttagcc ggcgaatcca
      301 tctggaagga aaggtggtct gactgcctgg agtgtgccga gcatggcatg gtgcaagtct
      361 gcctcttacg ccgcaccctt ggtataagac acgagctgta gacagaacgt tctgaaccgg
      421 ccgtctcaac aaaggattag ccacaattag cttctgctgg tcaatcttga gtagttttgt
      481 actcgggtca acttctgccc ttctgtggcg cgaaggcgcc cacgcagtct gaattcacca
      541 gctgccctta gacttatgta ggatggcggc tcattgtcag gctccgactg agcaatgatg
      601 ccgctttgcc gctttgatat cttaaatttt tgcagcttgc tacttcgcct tatagtactc
      661 taaggcaaaa aaggagggaa agctgatgca gtcactatgc aagccgtttc caaccggctt
      721 gcatatcatg atggaatcag cagaatccct tcaagtcacg tgcgcttctc ttgctgccgc
      781 agttatccct gtcagacgac acctaaggga tcataagtct gtccgaatag ttccggatag
      841 aaccagccct ccgcatcgat gaaaggagcc acagcttcgg ggatagcctc aggcgcatca
      901 atccagccca gggcttcgat ttgcgtgccg cttttccatt tgaccttcac ttgcagacca
      961 tccagcgaag cgacatcgtc actcttggcc ccgcacaaag tagcgatgac ggcagccaaa
     1021 agctcgactt ttcgctgcca gtcgcgctgc ccaacgccca ggccgaagcc ggtaacgaaa
     1081 tcttttccaa ccagttcaaa gtagacgccg gggttgttta agagaacttc cagacggccg
     1141 ctctgcggtt gtctaatctt caaagtatca atcggttcat tagctttcat aagtttcccg
     1201 tgattgttta gtgtcatcga ctcaatcgca gacgggcggc agatgcagcc cgtctgcaaa
     1261 atatcgctag ttcgaatcag tcggcttccg gaagttcggg acagctacag cggcttttct
     1321 tcaaaagcca gcgcaccgag aatgccagaa acagcaccac gaaggcaaac cacggccagt
     1381 gcggtatcga ggcgatgccg aaccagaccg ttaatccaag ccagctccag atacctgaaa
     1441 gattcagaat attgacgccg gctatgttgt taatcccggc tgaggaccat ccggtccaga
     1501 gcacaagaca gaaaacggaa agcccggcaa tccagatggt aactggtcca gcccaggcta
     1561 ttcctaagca tacggcaatc gataccatga atgccgcaaa taaagtaaga aacgcatttt
     1621 cattgttcat gagagtctct atcgggtaa
//
LOCUS       DAOQNZ010001047         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_56588_length_1648_cov_3.216573, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001047 DAOQNZ010000000
VERSION     DAOQNZ010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56588_length_1648_cov_3.216573"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..1548
                     /locus_tag="PKW73_16220"
     CDS             403..1548
                     /locus_tag="PKW73_16220"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA74897.1"
                     /translation="MQGDKSPKRELKVKLGRETYLNAYMMLLIMLILLFLTGWALFHY
                     QMPGWVIGLTESLLVIFLFLLLLFGGFVASLIAAARRLNVAALVAQMDLVLGRPMRKL
                     IIFDFGMTRLSLLILANKAIFDGQLLDAEALLKPLFDVPEEDPVASNFAFDCLEKGLM
                     AHVFAWTGRKKEAERMLEATISAAEDAYGRDQGKDQSIMLAEVYCNTGCMLGLLEDKE
                     RALGVLSRGLSMREQIYGAETEEAAKALNNLGQVQLEAGDLTGAEVSLRRARKILETD
                     RKDTSGGAVLGYVLDTLATVLLELGQSEEAYELSARALKLGQLDVHERAARLFTMGRC
                     LEKRGDLRRAINYHDRALAGWSKMQGFEHPLVERCRKNRESLASRRN"
BASE COUNT          347 a          382 c          488 g          431 t
ORIGIN      
        1 tgccggcttg gagacggtgg ccttcccgga gccggaatag gcttgccgta ctggttccgc
       61 taatttgggt caggctttgc cagcttctta aaagggcatg gcggattgtg attttgggag
      121 aagtcccatc cttgccgatt gcaacacgat tagcggtggt ttgccagtgg cgatttgtct
      181 gaaaatggct tgcctggctg tttgtggctc ttttgtgaca tctgctcaga attgctaaga
      241 agcggcttga aaagggccgt tgccggttca tttctgttat ggtgttgttc tcaggtgcgc
      301 ctgcggcggc aacgagggaa tttttctatt cggctctacc gtggctcatt tttcacttcg
      361 gaaccattga ttgccaattg ttcctatggg cataagcagt ttatgcaagg cgataaaagt
      421 ccaaaaagag agctcaaagt caaactcggg cgcgagacat atttgaacgc ctacatgatg
      481 cttttgatca tgctcatcct cctctttctc acgggctggg cgctttttca ctatcagatg
      541 cctggttggg tgattggtct gaccgagagt ctgctggtaa tttttctctt tttactacta
      601 ctcttcggtg gctttgtcgc ttctctcata gctgcggcca ggcgcctgaa cgtggctgcc
      661 ctggtggctc agatggatct ggttttgggc cgaccgatgc gcaagctgat tatctttgac
      721 tttggcatga cgaggctttc cctcttgatt ctggccaaca aggctatttt cgatggacag
      781 ttgcttgatg cggaggcact tctgaagcct ttgtttgatg ttccggagga agacccggtg
      841 gcttccaact ttgctttcga ttgcctggaa aaggggctca tggctcatgt tttcgcctgg
      901 acaggtcgca agaaggaagc ggagagaatg ctggaagcga ccatcagtgc cgccgaggat
      961 gcttacgggc gagatcaggg taaagaccag agcatcatgc tggcggaagt ttactgcaat
     1021 accggctgca tgctggggct tctggaagac aaagagcggg ccctgggagt cttgtcccga
     1081 gggctttcca tgcgtgagca gatttatggc gctgagacgg aagaggcggc caaggctctc
     1141 aataatctcg gtcaggtgca actggaagcg ggagacctga ctggagcgga ggtaagtttg
     1201 cgacgggcca gaaagatact ggagaccgat cgcaaagaca cctccggtgg tgctgtactc
     1261 ggctatgttc tcgatacact ggccacagtt ttactcgaac tagggcagag cgaagaggct
     1321 tacgaacttt ccgcccgcgc tcttaaactt ggtcagttgg acgtacatga acgggcggcc
     1381 agactcttca ccatggggcg ttgtctggag aagcgcggcg atttacgccg agccattaac
     1441 tatcatgatc gggctctggc cggttggtcc aagatgcagg gctttgagca tccactggtg
     1501 gagcggtgca ggaaaaatcg agagagcctg gcttccaggc gcaattaaca gggggattgc
     1561 aatgcctgga gaactggacc taaatcacgc caatttagac gatgagaata taccgatcgc
     1621 ttatgccgta gctttgccga agtttatt
//
LOCUS       DAOQNZ010001048         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_56667_length_1647_cov_2.757538, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001048 DAOQNZ010000000
VERSION     DAOQNZ010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56667_length_1647_cov_2.757538"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..890)
                     /locus_tag="PKW73_16225"
     CDS             complement(72..890)
                     /locus_tag="PKW73_16225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNA74898.1"
                     /translation="MMEAAKSLFLPFISVAAVCVVAELAWSFHKKRQVYNLKETLCNL
                     SMLVINRSTQFLFVGYTYYSITWLEQFQPHKLPDTPLFALLAVIVVDLLYYIEHRALH
                     TNKLLWCLHQVHHSSPWFNFTASFRLHWLGRIVTPVFFAPAVFLGFRVEQILLFVTLN
                     LIYQFFLHTEMVGKLGFLEGIINTPSAHRVHHGQNKQYIDKNFGGLLMVWDRLFGTYE
                     PEVETVKYGVTEGYFGANPFVVQSVLFRRLLRELIRDLLGKAKVITPKRTRTDA"
     gene            1096..>1647
                     /locus_tag="PKW73_16230"
     CDS             1096..>1647
                     /locus_tag="PKW73_16230"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNA74899.1"
                     /translation="MTETAAEPRKSWAALEIEVKRTDEDLACWLLMQCGAQGCEVEGL
                     DGQPAEGGKVRVRATFDSAVIAQSDFDSIIGSFEEYGLAESLKTLKRKDVPEEDWLAR
                     WKEGFEPFRVGTMFIVAPSWYHPQADSECAGKLDSELVGNRKVIYIEPGMAFGTGLHA
                     TTQFCLRALESFPPRKRILDVGTG"
BASE COUNT          405 a          405 c          455 g          382 t
ORIGIN      
        1 atctatttgg cctccaagcc ctggagacaa tatgtcatgg tcgagccggc tttttagccc
       61 ctatccatca cttaagcatc tgttcgggtg cgtttgggag taatcacttt cgcctttccc
      121 aggaggtctc ttatcagctc tctcaatagt cttctgaaca gcaccgactg caccacaaat
      181 ggattggccc cgaaatagcc ttccgttaca ccgtatttaa cagtttccac ctccggctca
      241 taggtgccga aaagtctgtc ccagaccata agtaagccgc caaagttttt gtctatgtac
      301 tgtttgttct gaccatgatg aacccggtgg gctgagggcg tattgatgat accttcaaga
      361 aaaccaagct tgccgaccat ttctgtgtgt aggaagaatt gatagatgag gttgagggta
      421 acgaaaagca aaatctgttc tacccggaag cctagaaata ctgccggtgc aaagaagacg
      481 ggcgtgacta tgcgcccgag ccagtgcagg cgaaagctgg cagtaaagtt aaaccagggg
      541 ctgctatggt gcacctgatg aaggcaccac agtagtttgt tcgtatgcag cgcccggtgc
      601 tctatgtaat agaggaggtc aaccacaatt acggcaagca gggcaaacag gggcgtatcc
      661 ggcagcttgt gcggctgaaa ctgttccagc caggtgattg aataatatgt gtagcccaca
      721 aacaggaatt gcgtagagcg gtttattact agcatcgata gattgcagag ggtctctttc
      781 aggttgtaaa cctggcgttt cttgtgaaat gaccaagcaa gttccgccac aacgcaaact
      841 gccgccaccg aaataaaagg taaaaataac gacttggctg cttccatcat ctgactatcc
      901 ctcaaacctt atctagtggg gcttttgcct gtcttgaacg ctcaggcgta cagacccatg
      961 agctgattac gggggcggtg ccaaaaagtt tcagcctgcc gggctaatcc agattaaatc
     1021 catgggttat attaagccgg tacggaatac ccgattggaa ttaagcggct attaattggc
     1081 cattaagagg caagcatgac cgagacagca gcagaaccca gaaagagctg ggccgccctg
     1141 gaaattgaag tgaaacggac cgatgaagac ctggcctgct ggcttttgat gcagtgcggc
     1201 gcccagggct gcgaggtgga agggctggac gggcagccgg cagaaggcgg caaagtaaga
     1261 gtacgcgcta ctttcgacag tgcggtcatt gcccagagcg acttcgacag tatcattggt
     1321 tcctttgaag aatacggtct ggctgagagt ctcaagaccc tgaagcgcaa ggatgtgccg
     1381 gaggaagact ggctggctcg ctggaaggag ggctttgagc ctttccgcgt gggtaccatg
     1441 ttcatcgtgg ctcccagttg gtaccacccc caggctgata gcgagtgtgc cggtaagctt
     1501 gatagcgagc tggtgggcaa tcgcaaggtt atctatatcg agccgggcat ggccttcggc
     1561 accggtttgc acgccactac ccagttttgt ttgcgggccc ttgagtcttt cccgccccgg
     1621 aaacgcatcc tggatgttgg caccggc
//
LOCUS       DAOQNZ010001049         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_56700_length_1646_cov_4.610308, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001049 DAOQNZ010000000
VERSION     DAOQNZ010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56700_length_1646_cov_4.610308"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKW73_16235"
     CDS             <1..441
                     /locus_tag="PKW73_16235"
                     /inference="COORDINATES: protein motif:HMM:NF017317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNA74900.1"
                     /translation="PRIANRLVRLVRDFGHYQGAEIIDGATASAALDSYQVDALGLDV
                     TDRRLIEIIIDNYDGGPVGLETLAATIGEDSDTLEDVWEPFLIQRGFIQRTPRGRMAT
                     AFAYQHLGRAMSATVSSKILLNSEVKQLDLFAEKLPPQSGQAEV"
     gene            449..1507
                     /locus_tag="PKW73_16240"
     CDS             449..1507
                     /locus_tag="PKW73_16240"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNA74901.1"
                     /translation="MSDTEEFVPGKVLSVGPAQVNAELQKSTGMISRRETAQIEILGG
                     VCQGEKVEVFNEITDNPVFNINLSPGKEVLLIVNLAGGKKEINIADYHRVPALSVLLI
                     VFLVAFVFFGGKKGVKSLIALVISVLLIAFVLLPLSLAGFDPLAVSIGICIATTLATM
                     YFVAGWTKKALAAVLGTVGGVFVAGLAAELVILLAPLTGLSSEEAQILRGSVLVRSPS
                     FYQGLLAAGMLIGALGVIMDVGISIASTVTEVSLAGQGLGWKKLYEAGMNVGRDIMGT
                     MTNTLILAYTGSALPLLLLISQMPLNKLINLDLVATEVASAISGSLGLVLTIPLTAFF
                     AARLMAAGSPGGQGNSSG"
BASE COUNT          319 a          424 c          473 g          430 t
ORIGIN      
        1 ccccgtattg ccaaccgcct ggtgcggctg gtgcgcgact tcggtcacta tcaaggcgct
       61 gagattattg acggcgctac ggcttcggcg gcccttgatt catatcaagt agatgctctc
      121 ggtctagacg tcacagaccg ccgcttgata gaaattatta tcgataacta cgacggtggt
      181 ccggttggtc tggagactct cgccgccacc atcggtgaag acagtgacac actggaggat
      241 gtctgggaac cgttcctgat ccagcgcggc ttcattcagc gcacccccag aggtcgtatg
      301 gctacagcct ttgcctatca gcatttggga cgggccatgt cggccaccgt ctccagcaag
      361 atcctgctca atagcgaggt caagcaactc gatctctttg ccgagaagct gccgcctcaa
      421 tccgggcaag cagaggtcta gggtttcgat gagcgacacc gaggagtttg tgcccggtaa
      481 ggttctttca gtgggaccgg ctcaggttaa tgccgagctg caaaagtcta ccggcatgat
      541 ttctcggcgg gaaaccgctc aaatagagat tttaggcggc gtctgccagg gcgagaaggt
      601 ggaagtcttc aacgagatca ctgataatcc tgtcttcaat atcaatttga gtcccggcaa
      661 ggaagttctc ttaatcgtca atctggccgg cggtaaaaaa gagatcaata ttgccgatta
      721 ccacagggtg ccggccctga gtgtattgct gatcgtcttt cttgtggcct ttgtgttctt
      781 tggaggcaag aagggtgtca agtcactgat agcactggtg ataagtgtcc tattgatcgc
      841 cttcgtgctc ctgccgctca gcctggcggg ctttgaccct ctggccgtct ctatcggtat
      901 ctgtattgcc actacccttg ctaccatgta ctttgtcgcg ggctggacga agaaggcact
      961 ggctgcggtg cttggcactg tgggcggtgt gtttgtcgcc ggtctcgccg ccgagctggt
     1021 gatccttctg gctccgctta ccggcttgtc ttcagaggaa gctcagattc tccgaggttc
     1081 ggtactggtg cgcagtccct ccttctatca gggactactg gctgccggta tgttgatcgg
     1141 tgccctgggg gtgatcatgg atgtgggaat ctccatcgct tccacggtga ccgaggtgtc
     1201 acttgccggt cagggactcg gttggaaaaa gctttatgaa gcaggtatga atgtcggacg
     1261 tgacattatg ggcaccatga caaataccct tattcttgcc tacaccggca gtgctctgcc
     1321 gcttttgctt ctcatttcac aaatgccttt gaacaaactt attaacctgg acctggtggc
     1381 gactgaggta gcctcggcaa ttagtggtag tctagggttg gtattgacca taccgctaac
     1441 ggccttcttt gctgcccgcc tcatggcagc aggcagccca ggcggacagg gaaattcgtc
     1501 cggatagtaa ggcttggcat gagactttta tttggctggt tactattttt cctggcggtg
     1561 gccagcatta tggccggcgg aaagtttctt gccctggttc tgggcgcggc ttgtttcatg
     1621 ggtggcaaag agttcattgc catggc
//
LOCUS       DAOQNZ010001050         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_57028_length_1642_cov_3.161311, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001050 DAOQNZ010000000
VERSION     DAOQNZ010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57028_length_1642_cov_3.161311"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKW73_16245"
     CDS             complement(<1..324)
                     /locus_tag="PKW73_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74902.1"
                     /translation="MATDVKHLPKDGGDDKSQPEAAVAAKVVQEFTPKDNHEQSQAAQ
                     KTRSDSNTAAVLPAVTIEEGKAKEKPVEKPPEKPADKPADKTPDRAALEKDAEALRQA
                     TGNDNY"
     gene            353..>1642
                     /locus_tag="PKW73_16250"
     CDS             353..>1642
                     /locus_tag="PKW73_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74903.1"
                     /translation="MSIYPVRDDFWTSSGLFPVLLQGAIYLSHSPNQNQESLRRIEMP
                     NEAQPRTEFRAEPGLFSRSTVGVDQTNSRLLDSLLVPESGAAKLPAGQGKILPSSAID
                     EILQEGSVESRQKQESQTGQIVAPTVLPSRNEAEAPVNPVQPAPVQERDPHFIYIDPP
                     ADGTGDGRASKPEIVVPVKREQSSVGQQLNGIVHNSATLFSSYKAFQFSRDRAETRLT
                     ANLDNPSKLVRPQVLSEFNNSRNSLLQSVESKSLEADTALAKLVAKKPNHFTHSYNVN
                     GEYFVVPNAEVKGLSGANLRLFENSRELGELKHGLNLDPKSMTAIKNRPSMELLNRDG
                     GFLRHGLDKEAAAYEAAADKLVQRGEFGQKRLLNGFRRELFSNGYVLASSFAINTVVD
                     NTVLSGSKTGVFTYGADILSPFILATGRHPLIKLGVMT"
BASE COUNT          353 a          425 c          453 g          411 t
ORIGIN      
        1 gtagttgtcg ttgccggtgg cttgccgcag ggcttcggca tctttttcca gggcggcgcg
       61 gtcaggggtc ttatcggccg gcttgtcggc gggtttttcc ggcggttttt caaccggttt
      121 ttccttcgcc ttgccttctt cgatggttac cgcgggtaaa acggcggcag tatttgaatc
      181 gctgcgagtc ttttgagcgg cctggctctg ttcgtgattg tctttgggcg tgaattcctg
      241 cactaccttg gcggcaacgg cagcctccgg ctgcgattta tcgtcgccgc cgtctttggg
      301 gaggtgcttt acatcggttg ccacagaagg tgataccggc ccgaggggtt acatgtctat
      361 ataccccgtg agggacgatt tctggacatc ctcgggactt tttcccgtac ttttgcaggg
      421 ggccatttat ttgtcacaca gtcccaacca gaatcaggag agcctcagga gaattgaaat
      481 gcccaatgaa gcccaaccca gaacagagtt tcgagccgag cccggcttgt tttcccgctc
      541 cactgtcgga gtagaccaga ccaacagccg cttgcttgat tcgctgctgg tacctgagtc
      601 cggggccgcc aaattgccgg cagggcaggg caagatcctt ccctcctcag ccattgatga
      661 aattttgcag gagggttcgg tggagtccag gcaaaagcag gagagtcaga ccggtcagat
      721 tgtggcgccc acggtgctgc cgtccagaaa cgaggcggaa gccccggtaa acccggttca
      781 acctgcgccg gttcaggaaa gagacccgca ttttatttat atcgatcccc ctgccgacgg
      841 aactggtgac ggtagagcga gtaagccgga aatagtggtg ccggtcaaaa gagagcaaag
      901 ttcagtcggc caacagctga acggcattgt tcataatagc gctacccttt tttcatccta
      961 caaggctttc cagttttctc gggacagggc tgagactaga ttgacggcta atctcgacaa
     1021 tccttccaag ctggtcagac cacaggtttt aagtgaattc aacaactcac gcaatagcct
     1081 attgcagtcc gttgaaagta agagcctgga agccgatacg gcccttgcta agctggttgc
     1141 taaaaagcca aaccatttca cgcattcata caatgtcaac ggcgagtatt ttgtagtccc
     1201 caatgccgag gttaagggct tgtcgggggc taatttgcgt ctttttgaga acagccgcga
     1261 acttggtgaa ttgaagcacg gtctgaatct tgatccaaag tcgatgactg ccatcaagaa
     1321 tagacccagt atggaactgc tcaaccggga cggtggcttt ctccgtcacg gtctcgataa
     1381 agaggctgct gcttatgaag cggctgccga taaactggta cagcgaggcg agttcgggca
     1441 aaagcgactc ttgaatggat ttagaaggga gcttttctcc aatggctatg tcctggcgtc
     1501 gtctttcgcc atcaacacag tggtggacaa tacggtcttg agcggctcta aaaccggtgt
     1561 tttcacctat ggtgccgata ttctttcgcc ctttatcctg gctaccggtc gtcatccact
     1621 cattaagctc ggtgtcatga cc
//
LOCUS       DAOQNZ010001051         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_57243_length_1639_cov_3.374369, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001051 DAOQNZ010000000
VERSION     DAOQNZ010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57243_length_1639_cov_3.374369"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..282
                     /locus_tag="PKW73_16255"
     CDS             28..282
                     /locus_tag="PKW73_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74904.1"
                     /translation="MTNNTNGFTLVFIQNAKREATALANARRVPNFVLDESYAPIQIE
                     DSMFLVRGLQLGALKHSDVFSFCAESEMAAFCEQMTLGAF"
     gene            373..1029
                     /locus_tag="PKW73_16260"
     CDS             373..1029
                     /locus_tag="PKW73_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74905.1"
                     /translation="MKNQHDEPLALPDKQAVRLYHCMPLSGVSYGVLLPDPAFSDTDF
                     LPAYRWAEERLGFFPLFLAVGPEEAVSMTGYVNQWQRYIGTERKDDGTIANVYRRKGE
                     FPNNVLFVFGKEAVAAHEAVFTDYMAWHIVLNETMNQHLHEAKIGVQAVRSLFKKSWS
                     MARWYNHSRKALHDVQLLVPRLDLRQAQEVWVRNKSTARKLKAMGFANVKVKRLPVER
                     "
     gene            complement(1281..1493)
                     /locus_tag="PKW73_16265"
     CDS             complement(1281..1493)
                     /locus_tag="PKW73_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74906.1"
                     /translation="MECILAEIALELVPLLGMVMRHGLIMPSLNRAVKERQRTNRLLE
                     LRSRRLKELEAQGVDLMEFYRRLAKT"
BASE COUNT          367 a          415 c          444 g          413 t
ORIGIN      
        1 gcctcaaaaa aaacaaggaa ttaactaatg acgaacaaca ccaacggctt cacgctggtt
       61 ttcattcaga acgcaaaaag ggaagcaacg gctctggcca atgctcgccg tgtccccaac
      121 ttcgttctcg acgaaagcta tgctccgatt cagatcgagg acagcatgtt tctggtccgc
      181 ggcctgcaac tcggggcttt gaagcactcg gatgtgttca gtttctgcgc ggaaagcgag
      241 atggcggcgt tttgcgagca gatgacactc ggagcgttct agaggtctca catggggttt
      301 gaccggtgtg agcacagatt gagggatcac tagaaacctt tctgtattta ctttcaggag
      361 cagtccatag ttatgaaaaa tcaacacgat gagccactcg ccctgcccga taagcaggcg
      421 gtgcggcttt accactgcat gcccctgagc ggcgtgtcct atggagtctt gctccctgac
      481 ccggcctttt ccgataccga cttcctgccg gcttatcgct gggcggagga gcgacttgga
      541 tttttcccgc tcttcttagc ggtaggcccc gaagaggcag tctccatgac tggctacgtt
      601 aaccagtggc aacgctatat tggaactgag cgcaaggacg atggcaccat tgccaatgtc
      661 tatcgacgca aaggtgagtt ccccaacaat gtgctgttcg tcttcggcaa ggaggcggta
      721 gcggctcatg aggcggtgtt taccgactac atggcctggc acattgtgct gaacgaaacc
      781 atgaatcagc atctccatga ggcgaagatt ggtgtgcagg ccgttcgatc cctgttcaaa
      841 aaatcgtggt cgatggcgcg ctggtacaac cattcgcgca aggctttgca tgacgtgcaa
      901 ctgctggtac cccggctgga cttgcgccag gctcaagagg tctgggtgcg taacaagtcc
      961 acggcacgta agctcaaggc gatgggcttt gccaatgtga aggtgaagcg cctgcctgtg
     1021 gagcgttagc aagaggagca gccaggctgc attctcttgc gtcatgagcg agatagggtc
     1081 gagtacctgg accctgtctc ggctcatgaa gctccggtag tgtttgaccg gcagaaagca
     1141 tgctttctgc cggtcctttt caattgcgaa ttccgaaaag ttttttgcgt tcttcaaaaa
     1201 ctactggtga tagtagaagc gttcaaaaat tttagtttga gcggaaaaga gaccgtccct
     1261 ggtctccttt cttcagacaa tcaagttttt gccagacgtc ggtagaattc catcaaatca
     1321 acaccctgtg cttccagttc tttcagccgg cggctgcgca actctagaag cctgttggta
     1381 cgctgtcttt ctttgacagc cctgttgagt gagggcatga tcagtccgtg tctcatgacc
     1441 attcccagaa gcggcacgag ttccagcgct atttcagcaa gaatgcattc cacggtttac
     1501 ctcattttgg tgaaggatgt ttgtttggtc tttattgaaa agccttgagt aatggtcaag
     1561 ggccgaccac ggtaaaagct gccagtgcct cggcttcttt cgttttgatt gcttcgagcg
     1621 tgagccagtt ttcgtgcct
//
LOCUS       DAOQNZ010001052         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_57303_length_1638_cov_3.900190, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001052 DAOQNZ010000000
VERSION     DAOQNZ010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57303_length_1638_cov_3.900190"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1250)
                     /locus_tag="PKW73_16270"
     CDS             complement(231..1250)
                     /locus_tag="PKW73_16270"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74907.1"
                     /translation="MRCNNYFDDENNVCPQDRAALEYVGREPLIGALINNRYAVDCVA
                     GKGASGIVYKATRLMMGGFVAVKVLHTYQGADSKSLEKFSREITALEQLRHPNIVSFF
                     DSGITDDGQPFLVMDFLEGTSLTTIIQQGPLKPSSVLNITLEICKALQFAHDQGFVHR
                     DMKPDNIVLDRAGGQDVVKLLDFGISYTIFENRMNTIARPTTVAGSPAYMSPEQCRGL
                     PLDHRTDIYSLALVVYEMFTGRKPFKANSNKEFMVKTVNEKVPLMATVRPDLGIPEPV
                     DLVIARALQKNPKDRHSSITVFASELTKSCEGSGFAFTKETAEERDDRLQNPGNRVSE
                     RLRGQ"
     gene            complement(1447..>1638)
                     /locus_tag="PKW73_16275"
     CDS             complement(1447..>1638)
                     /locus_tag="PKW73_16275"
                     /inference="COORDINATES: protein motif:HMM:NF036806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74908.1"
                     /translation="PVQVELFGREKEVFDLIAAEPLIHFDKIAELMQMPAGELSSALI
                     MLELAGAVERMNGDLYQTA"
BASE COUNT          413 a          430 c          393 g          402 t
ORIGIN      
        1 cgccggtaca ttagtgccgg tgccgctagg ctttttcaca agagcagaca gcttctcgga
       61 aatatccgcc ttgggtgcag gtacttcgcc tttagcctgg ttatttccgc ctttcttact
      121 caagtaaggc gtcaaatctg gcatgtcatc cttcaaacca atcatcctct cagaagcacc
      181 gtagcgacaa agctcacatt tatcagacca acatcattac ttagagcgat ttactgcccc
      241 ctcaatcgct cacttacccg atttccagga ttttgcaacc tgtcatccct ctcttccgcc
      301 gtctctttag taaaggcaaa cccggaaccc tcgcaggact tagtcaattc ggaagcaaaa
      361 acagtgattg aactatgcct gtctttggga ttcttctgaa gagctcgagc gataaccagg
      421 tccacaggct ctggaatacc caaatccggc ctgacggtag ccataagcgg cactttctcg
      481 tttacagtct taaccataaa ctctttgttg gaattagcct tgaaaggctt gcgcccggtg
      541 aacatctcat aaaccacaag agcaagagaa taaatgtcgg tacgatggtc cagaggcaaa
      601 ccacggcact gctcgggact catataggcc ggcgagccgg ctaccgtggt cggtctggcg
      661 atggtattca tgcgattttc aaagattgtg taggaaatgc cgaagtccag gagtttgacc
      721 acgtcctgcc cgccggctcg atcaagaacg atattgtccg gcttcatgtc ccgatgcaca
      781 aaaccctgat cgtgagcgaa ttgcaaggct ttgcagatct ccagggtgat attgagaacg
      841 gaggaaggct tcaaaggtcc ttgctgaata attgtggtga gagaagtccc ttccaaaaag
      901 tccataacca gaaagggctg accgtcatcg gtgatgccgg agtcgaagaa actgacgata
      961 ttgggatggc gcaattgctc cagggcggta atctccctgg aaaacttctc cagactcttg
     1021 gaatcagcgc cttgataggt gtgcagaacc ttgactgcta caaatccacc catcatcaat
     1081 cgggttgcct tgtagacgat accggaagcg cccttgccgg ccacgcaatc caccgcgtag
     1141 cgattgttga tcagagcgcc gataagcggc tcccgcccga catattcgag ggcagcacga
     1201 tcttgcggac agacgttgtt ttcgtcgtcg aagtagttat tgcatcgcaa gcacaacttc
     1261 atttttggtg ggctaaccgc tctggaacat taggaaggac atttaaaagt caagggctac
     1321 aagggggagg gaaggggagc cgacgtggac cccgcttttt taacttaccc aaaactgcct
     1381 ctcttccatc gccggcttgg catatatgga taagtgttag ttaaattatc agcttggcaa
     1441 gtcgattcaa gccgtctggt acaagtcacc attcattctt tccaccgccc ccgctaattc
     1501 cagcatgatt aaagcgctgg atagctcgcc tgcaggcatc tgcattagtt cggcaatttt
     1561 atcgaagtga atcaggggct cggctgcaat aaggtcaaat acctcttttt ctcgtccgaa
     1621 aagttcaacc tgcacagg
//
LOCUS       DAOQNZ010001053         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_57759_length_1632_cov_2.889664, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001053 DAOQNZ010000000
VERSION     DAOQNZ010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57759_length_1632_cov_2.889664"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKW73_16280"
     CDS             complement(<1..379)
                     /locus_tag="PKW73_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74909.1"
                     /translation="MQLGIEHSEYLPPISQDSGTDQSRHPVSLLSSNATRYGAQQYYQ
                     SRPQKMVEWFQIPGWMAGTWSKQGDVTVSATDLVTGYSTKMNEWIDNRMTVSFGHQVD
                     RSGTIWQANILPAETDSMSGAKSV"
     gene            complement(456..1313)
                     /locus_tag="PKW73_16285"
     CDS             complement(456..1313)
                     /locus_tag="PKW73_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74910.1"
                     /translation="MAKSQQIKYVPKQPQPKVEAPVMPELSDSKNKTCYQIFWGVLVG
                     GELLSLAMIYFGVRSLMESTGSEPNWTAIVLASVMIIFALITAVGSLRAALVMAVMMA
                     ARTYSYEACGKFCEKAISLRAFIPGGVAWAIQTLLEFKVQKGEFDEVIAVGTKEYEAA
                     LAKNAKEYGYGSVCACVGTAYSMRSELHKAAEWLEKSTAQYKAMFASLEKDKKVAKIP
                     GGADNVYLRYAESLLGLSGTYLRLNDKRNAKERLSSVYEITKKVKDCPEKEVLLKRTQ
                     EISKHLKHW"
BASE COUNT          399 a          442 c          408 g          383 t
ORIGIN      
        1 gcacgctctt ggccccggac atggaatcag tttcggccgg cagaatgtta gcttgccaga
       61 tggtgccgct tcgatccacc tggtgaccga aggagacggt catacgatta tcgatccatt
      121 cgttcatttt cgtgctgtaa ccggtgacca ggtccgtggc gctgaccgtc acgtccccct
      181 gtttgctcca ggtaccggcc atccagcctg gtatctgaaa ccattccacc attttctggg
      241 gtctgctctg atagtactgc tgggcaccgt atctagtcgc attcgagctc aagagcgaaa
      301 ccggatgcct gctctggtct gtgccgctat cctgggaaat gggaggcaaa tactctgaat
      361 gctcaatgcc caactgcaag gtttccgctc caacaggagc gaataatgac ataaggagac
      421 aggagagaag gggtagaaga cgccgggtca tttagttacc agtgtttgag atgcttgcta
      481 atctcttgag tccgttttag cagtacttcc ttctcgggac aatccttcac ctttttggtg
      541 atctcataaa ctgaagaaag cctctctttg gcattgcgct tatcgttcag gcgcagataa
      601 gtaccgctga gaccaagcag gctctcagca tagcgcaaat aaacgttgtc ggcaccgccc
      661 ggaatcttag ctactttctt gtccttctca aggctggcga acatggcttt gtactgagcg
      721 gtcgactttt ccaaccattc cgctgcctta tgcaattccg atcgcatgga ataggcggtg
      781 ccgacacaag cacaaacact gccatagccg tactctttag cattcttggc gagggcggcc
      841 tcatactctt tggtgccgac ggctattacc tcatcgaact cacccttctg caccttgaac
      901 tccagcaagg tctgtatggc ccaggccacg ccgccgggaa taaaggcgcg cagggaaatt
      961 gctttctcac agaatttacc gcaggcttca taagaatatg tgcgggcagc catcatcaca
     1021 gccatcacca gagcagccct gagactaccc acagccgtaa tcaaggcaaa aatgatcatc
     1081 acggaagcaa gcactatggc ggtccagtta ggttcggacc ccgtcgactc cataagcgag
     1141 cgtacaccga agtagatcat ggccaaactg agcaattcac cgccgaccag caccccccag
     1201 aagatctgat agcaggtctt attcttagag tcggaaagtt ccggcataac cggcgcttcc
     1261 accttgggtt ggggctgctt gggtacgtat ttgatttgct gagactttgc catctgtgtt
     1321 taattttcca ccacttcaca actatccatt gtatctatta aaggggttgg aaaccttagt
     1381 gatttcaaag ccggccccgg cagccaaaag tcttccgctc ccggcagcca cgggctcccc
     1441 ggctcgggac cgccggaccc gcgagaaggc agaaatatgg ggtattgaac tgcttaacaa
     1501 tgttccgaag aatccgcttt ttgaccgata gctgataaac ggccaaggaa atacacttaa
     1561 gttagagaaa ggaggctcag gcaaaggagt aagttatgga caagatcagt aagggagacc
     1621 caaaggcaac tg
//
LOCUS       DAOQNZ010001054         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_57801_length_1631_cov_4.448604, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001054 DAOQNZ010000000
VERSION     DAOQNZ010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57801_length_1631_cov_4.448604"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PKW73_16290"
     CDS             <1..1206
                     /locus_tag="PKW73_16290"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNA74911.1"
                     /translation="GDLAIETSEDPSFSRGDLASLILSLRNFDGGKSGIRLNGIRGKI
                     YQLSPAGSADNFHVHWLLEDFGQEWMPVSSSLVVDKNIAFTGGIPSTMKIEDGETGVD
                     ANLNAVLDGVLAADLASAFLLFDEKTQTVKLFRGLAIGPNGKHDAKVHKDGPFVVGNP
                     TAFNMALFMNALSESGLNFEHKIYKGEKIPGFLAKQPAQVMAEHSSKGLPQLIRLCLY
                     ESDNLYAQQFLRTIAGTRSDGQGHLSKNAKQAHSTMTLEDRGLARMGEWLSAIAVPNA
                     EVVLFDGCGLSRKNSITPHALNLVLRYMQKKHPEYIELMRVSDSKGGGRFHFKTGAME
                     TVRGLSGVLETFEGRKLAVTALVNGHTASVKDVRMVLGDLIERLRRLPLPAPPAAAAP
                     TKVSVPEKQ"
     gene            complement(1221..>1631)
                     /locus_tag="PKW73_16295"
     CDS             complement(1221..>1631)
                     /locus_tag="PKW73_16295"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA74912.1"
                     /translation="VMTDQSHLSVLRMTMDTAPLPLLPQLSYTVESRYEQNEKGGRIE
                     FRRIAGTLKDFRGFWEMSPAHGGTKTQLTYSMFLDPGFFVPQWIVREGVKSELPRTLV
                     ALRKRIKAVYEDQQRPESHTILAANLNASHHNLH"
BASE COUNT          369 a          402 c          454 g          406 t
ORIGIN      
        1 ggagacctgg ccattgagac cagcgaggac ccatccttca gccggggaga tctggctagt
       61 ttgattttgt cccttcgcaa tttcgacggc ggtaaaagcg gtattcgttt gaatggtatc
      121 agaggcaaaa tttatcaatt gtctcccgcg ggtagtgccg acaattttca tgtgcactgg
      181 cttctggaag actttggaca ggagtggatg ccggtttcct ccagtttagt ggtggataag
      241 aacatagctt ttacgggcgg cattcccagc accatgaaga ttgaagatgg tgagacgggc
      301 gtggatgcta acttaaatgc agttttggat ggagtcctgg ccgcagacct ggcttccgct
      361 tttttattgt ttgatgagaa aacccagacc gttaagcttt ttcgtggact ggccattggt
      421 ccgaacggta aacacgacgc caaggtacat aaggacggtc cctttgtagt cggtaatccc
      481 actgccttca acatggcttt gtttatgaat gctttgagcg agagcggcct caatttcgaa
      541 cataagattt ataagggtga gaaaatccct ggtttcctgg ccaagcagcc tgcacaggtg
      601 atggccgagc attcctctaa ggggcttcct caactgatca ggctctgcct ctatgaaagc
      661 gacaatttgt acgcccagca atttttgcgc actattgccg gtaccagaag cgatgggcaa
      721 ggccatctaa gcaagaatgc caagcaagcc cacagcacga tgaccctgga ggaccggggg
      781 ctggctcgca tgggtgaatg gctctctgcc attgccgttc ctaatgccga agtggtgctc
      841 ttcgacggct gcgggctgtc tcgcaaaaac tccataactc cccatgccct gaatcttgtt
      901 ctgcgttata tgcagaagaa gcatcctgag tacattgaac tgatgcgcgt aagcgacagc
      961 aagggcggcg gacgctttca ctttaagacc ggcgccatgg agactgtcag aggtctcagc
     1021 ggcgtgttgg aaaccttcga gggacgtaag ctggccgtca cagccctggt gaacgggcac
     1081 actgcttccg tcaaggatgt acgaatggtt ctcggtgact taatcgaaag attgcgccgt
     1141 cttcccctgc ctgctccgcc tgcggctgcg gccccaacta aggtaagcgt gcctgaaaag
     1201 caataattta gccggcgcag ttagtgtaaa ttgtggtggc tggcattaag attcgcggcc
     1261 agaatggtat ggctttccgg tctctgctgg tcttcataaa ctgctttgat gcgtttgcgc
     1321 aaggctacta gagttctcgg caattcgctc ttaacgccct ctctgacaat ccactgaggc
     1381 acaaagaagc ccggatcgag gaacatgctg taggtgagct gagtcttggt gccaccgtgg
     1441 gcggggctca tttcccagaa acctctaaaa tctttcaagg ttccggctat gcgtctgaat
     1501 tcgatgcgcc cgcctttttc gttttgttcg tagcgagact cgacagtata ggagagctgg
     1561 ggcaaaaggg gcagaggcgc tgtatccatg gtcatgcgca gaacggacag atggctttga
     1621 tccgtcatta c
//
LOCUS       DAOQNZ010001055         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_57814_length_1631_cov_3.734772, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001055 DAOQNZ010000000
VERSION     DAOQNZ010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57814_length_1631_cov_3.734772"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKW73_16300"
     CDS             complement(<1..816)
                     /locus_tag="PKW73_16300"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNA74913.1"
                     /translation="MAELESGAESKDKDQKLIRSQADLARNLGGQECNPQPGSKQLPV
                     MEAGQMDIGCNYKDLSEAGAQAENNLDLLSLYNQARPSTVHFQLKRDGTVLGGGTGAM
                     VKAEENTCKILTDNHVVSEHTKQTYKLDEIKVVTHDGTIYDAKAYKADPGKDLAVVEI
                     ATGDKTAQICKPIEFVDTPVTFKERDRAVTIGQPYGTGAFYTSEGTFNSQISRQDAVT
                     KLRADKLPPNDNPDRRLDMFDMRGIRGYSGAPAYQLPLGKAEGEKPKAFGVVDM"
     gene            complement(1012..>1631)
                     /locus_tag="PKW73_16305"
     CDS             complement(1012..>1631)
                     /locus_tag="PKW73_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74914.1"
                     /translation="PQATAKTTFRKDFQTRNPVKSADSVESIIMGQTEKIAAHPYSTN
                     TPRLVDPEEIFDRTAPESPLDLWTKPRKYYKIKEAENAAANFTTSQKHLLQATNYSSA
                     NEREGRPPERAAQAAPSPSASPSEPLEIRIERLHGIVDRLNKLGKHTNDPSATASVLE
                     HLKQELETLESAAQSGKWQAVERSSTSIHKIRREFKSLLKESDLL"
BASE COUNT          288 a          385 c          455 g          503 t
ORIGIN      
        1 catgtccaca acgccgaatg cctttggctt ttccccttca gctttgccga gcggtagttg
       61 gtaagcaggc gcgccgctgt aacctctgat gccgcgcatg tcaaacatgt cgagccggcg
      121 atcgggattg tcattgggcg gcagtttgtc tgccctcagt ttggtaacgg cgtcctgtct
      181 gctaatttgc gagttgaagg taccttcgga ggtgtaaaag gcgcctgttc catatggttg
      241 acctatggtt accgcccgat cccgttcctt gaaagtgacg ggtgtatcca cgaactcaat
      301 cggtttgcaa atttgggcgg tcttatcgcc ggtggcgatt tccaccacgg ccagatcttt
      361 tcccggatct gccttgtaag ctttagcatc gtatatggtg ccatcatggg tgactacttt
      421 tatttcgtct agcttgtaag tttgctttgt atgttcggat accacatgat tgtccgtaag
      481 aatcttgcag gtgttttctt ccgccttgac catggcgcct gtgccaccgc ctagaaccgt
      541 gccgtcgcgt ttcaattgga aatgcaccgt ggaaggtcgg gcctggttgt agagactcag
      601 cagatcgaga ttgttctcgg cctgggcgcc ggcttcactc agatctttat agttgcagcc
      661 gatgtccatc tgtccggctt ccatcaccgg cagttgtttg gaccccggtt gcggattgca
      721 ttcttgtccg cccagatttc ttgctaagtc ggcctgtgag cgaattagct tttggtcttt
      781 gtccttagat tcggcccctg attcgagttc agccaatttg cttgtctcca ggctaaaagt
      841 tcttcgctgg tggatgattc cgcaagaaaa tggctcttca attgaagctt gtgctaaagc
      901 tagcagtgtt tacgtgaaag tttcgtaaat ttgtagttgt gaaggctggc tatagtgggg
      961 caagtacgaa gctttcagcg cagaattcta ctggtttggg atagctgaac tttacagaag
     1021 atccgattct ttcaatagtg atttaaattc tcggcgaatt ttatggatac tggtggagct
     1081 tctttctacc gcttgccact tgcccgactg ggctgccgac tccagagttt ccagttcctg
     1141 cttgagatgc tccagcacgc ttgccgtggc agagggatca ttggtgtgct tgcccaactt
     1201 gttgaggcga tcgactatgc catgcagccg ttcaatacgt atttccagtg gttctgatgg
     1261 ggatgcggat ggtgatgggg ctgcctgtgc ggctctctcc ggaggacgtc cctcacgttc
     1321 atttgcggaa gagtagtttg ttgcctgcag gaggtgcttt tgtgaggtag tgaaattggc
     1381 ggctgcgttc tcggcctctt tgattttgta gtacttgcgc ggttttgtcc acaaatcgag
     1441 ggggctttcc ggcgcggttc tatcgaaaat ttcctcgggg tctacaagtc tcggtgtgtt
     1501 tgtgctgtag gggtgtgcgg caattttttc ggtctgtccc atgattatcg actccaccga
     1561 gtctgcactc ttgaccgggt ttctggtttg gaaatccttg cgaaaggtgg ttttggccgt
     1621 tgcctggggt t
//
LOCUS       DAOQNZ010001056         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_57900_length_1630_cov_3.953016, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001056 DAOQNZ010000000
VERSION     DAOQNZ010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57900_length_1630_cov_3.953016"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="PKW73_16310"
     CDS             <1..1395
                     /locus_tag="PKW73_16310"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA74915.1"
                     /translation="PAPSGPAGQPPQTPPAPQPAEPYQQTPQQKPNAPQAAPAPLAPL
                     DSTGGFGIPVIGNPVQVPVQAQPPSAPVQPAPSRPAEAQDVPESGSLKQISLVKLLRL
                     YKAARVSGRLRLDTQDAAAVIYLHEGETLQALMGELKGDEALTEILLMSDGQFVLSRG
                     ISTANAARNIETPPEVLLANHKQMAYLLKELQDYGMQANSTFVRSDASLTKQEFLRIA
                     SQEAPMDPEIMSKLYIKLDGKQTLAELAKTAGLSRLRLIEALYHMVSFELVDIYMPPA
                     APRQLVVTPKKIDGAMIQSVMMSLRREDTGLFIYPAFLYFLEEEFFRVYRARGTMTVI
                     VFEMRDEVNTDGGVKRRVLPSVAIADAAGRIAGRKRHTDVVAHYEAYDYAVVLPGTGS
                     AGTKVFIKKIIKALTETPLAGMDGRKLVIAFGTATIPEDFTNLNSMLGAAELALEQAR
                     NQGKVLVQYRDLLS"
BASE COUNT          359 a          442 c          447 g          382 t
ORIGIN      
        1 ccagccccga gcgggcctgc ggggcagccg cctcaaacac cgccagcgcc tcaacccgca
       61 gaaccttacc agcaaacccc gcagcaaaaa cctaacgcgc cgcaagcagc gccggcacca
      121 ctggcaccac tggattctac cggcggtttc gggattcctg ttataggtaa tccagttcaa
      181 gttccagttc aagctcagcc gccgtcggct ccggtgcaac cggcgccctc ccgaccggcg
      241 gaagcgcagg acgtgcctga gtctggtagc ctcaagcaaa tttctcttgt gaaactgctg
      301 cgcctttata aggctgcaag agtatccgga cgactacgcc tggatacgca ggatgccgcc
      361 gcggtcatct atctgcacga gggtgaaacg ctgcaagccc tcatgggcga gttgaaaggc
      421 gatgaggcgc ttaccgaaat tctactcatg agcgatggtc agtttgtgct ctctcgcggt
      481 atttctacag ccaacgccgc ccgcaatatt gaaactccgc cggaagtgct actggccaat
      541 cacaagcaaa tggcttacct gctcaaggaa ttgcaagact acggcatgca ggctaactct
      601 acttttgtga gaagcgacgc cagtttgacc aagcaggagt tcttgcggat agcctcgcaa
      661 gaagcgccca tggacccgga gattatgtcc aagctttaca taaagcttga tggcaagcag
      721 accctggctg aactggctaa aactgccggt ctatcgcgtt tgcgtttgat tgaagctctt
      781 tatcatatgg tttccttcga gctggtagat atttacatgc cgcctgcagc acccagacaa
      841 ctggtggtga cgccgaagaa aatcgatgga gccatgattc aatcggtgat gatgagcttg
      901 cgccgggaag atacagggct ctttatttac ccggccttcc tctattttct ggaagaagag
      961 ttcttcagag tgtaccgggc acggggcaca atgacggtta tcgtttttga aatgcgcgat
     1021 gaggttaaca ccgatggtgg tgtcaaacga cgtgtcctgc cctccgttgc tatagccgat
     1081 gccgccggtc gaattgccgg tcgcaagcgt catacggatg tggtggccca ttacgaagcc
     1141 tatgattatg cggtggtgct gccgggcacc ggtagtgccg gtacaaaggt attcatcaag
     1201 aagatcatca aggcgctgac cgaaactcca ctggccggta tggatgggcg aaagcttgtc
     1261 atagcctttg gcactgccac cattccggaa gatttcacca atctaaattc tatgctgggg
     1321 gctgccgaac ttgcccttga gcaggctcgc aatcagggta aggtcttggt gcagtataga
     1381 gacttgttga gttagttagt ggaccgcgcc gtccgggtgt ttttgccagg cgaggtcgcg
     1441 ggtacaaact tctaagtgct tcggcggatt ttcatttggg gactgcggag tggaacaaat
     1501 tgcctccacc attcttttcc ggctcatggc cctgctcggc ctggccttga tctttgtgct
     1561 ctggagtttg tggttcgctt catacaagct gatttgctgg atcggctcgc tccccactca
     1621 tatgcaaata
//
LOCUS       DAOQNZ010001057         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_57903_length_1630_cov_3.820317, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001057 DAOQNZ010000000
VERSION     DAOQNZ010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57903_length_1630_cov_3.820317"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /gene="cobJ"
                     /locus_tag="PKW73_16315"
     CDS             <1..1592
                     /gene="cobJ"
                     /locus_tag="PKW73_16315"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: protein motif:HMM:NF014613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HNA74916.1"
                     /translation="KATVKTNGEGTDGSARKTGCLYLVSVGPGRSEYICPQAKEAISR
                     SDVIASYELYLTWVKPLLDKQKLITTPLTQERERAQISIDAARQGKKVSLLSSGDIGV
                     YAMASLVFELMQEDDQFQVEVIPGITAATACASIAGAPLSHDFATLSLSDLLCPMNWI
                     EERARAVGKADLCLALYNVQSEKRQEGVYKIIDILLKAGKNPQTLCAVVKNAYRPAQE
                     SYITTLSELRQKKFDMFTSIIVGNRFTRRKRNWIYTPRGYGDWQEAGPEPPQADAVWV
                     FSGTSDGNEAALKIKASGRKVVVSVASDYGAGINKQTMTDAAVPVICGSLGEAKRKEL
                     LKQSSCKAVVDATHPYAQKISKQLSDICAELNIPYLRLEREASRLPAHAVIAADFAEA
                     ATRAMELGERIFLSTGSKELPTFLSAPSAANKQWFLRILPDPDLLERAVALGIPRSNI
                     CAMQGPFSQAINEALWRDWRIDCVVTKDSGSKGGLSEKIKATENLALPLIVVARPPAA
                     SPRNTHKSVPQLIEALNHLNL"
BASE COUNT          429 a          478 c          409 g          314 t
ORIGIN      
        1 agaaagctac agtgaaaacc aacggcgaag gcaccgacgg aagcgcaaga aaaacaggtt
       61 gcctctacct tgtctccgtc ggccccggac gcagcgaata catttgtccc caagccaaag
      121 aagctataag taggagcgac gtgatcgcct cttatgaact ttacctgacc tgggtaaaac
      181 cgcttctcga caaacaaaag ctcatcacca caccactcac ccaggagcgc gaacgcgctc
      241 aaatatctat tgatgcagcc cggcagggta agaaagtctc gctcctctcc agcggtgaca
      301 tcggtgtcta tgccatggcc tctctggtct ttgaactgat gcaggaagat gatcaattcc
      361 aggtagaagt catccccggc atcacggcgg ccacagcctg cgcctccata gccggtgccc
      421 ccctctccca tgactttgcc accttgagcc tttccgacct actctgccca atgaactgga
      481 tcgaagaaag agcaagagct gtcggcaaag cggacctgtg cctggctctc tataacgtac
      541 agagcgagaa gcgccaggaa ggtgtgtaca agattatcga catcttgcta aaggccggca
      601 agaatcccca aacactttgc gccgtagtaa aaaatgccta ccgtcccgcc caggagagct
      661 atatcaccac cctctccgag ctgcggcaga aaaaattcga tatgttcacc agcatcatcg
      721 tgggcaaccg attcaccaga cgcaagcgca actggatcta tactccgcgc ggctacggcg
      781 actggcagga agccggaccg gagccgcctc aagccgatgc tgtctgggtc ttttccggca
      841 ccagtgacgg gaatgaagcg gcgctcaaaa tcaaagcaag tggtagaaag gtggtagtgt
      901 cggtagccag cgactatggt gccggcatca acaagcagac catgaccgac gccgcggtgc
      961 cggtcatttg cggcagtctg ggtgaggcta aaaggaagga acttctaaaa caaagttcct
     1021 gcaaagccgt tgtagacgcc acgcatcctt acgcccagaa gatttcgaag caattgagcg
     1081 atatctgcgc agaactcaat attccctatc tccgtctgga aagagaagcc tcccgcttgc
     1141 ccgctcacgc tgtaatagcg gctgattttg ccgaggctgc cacccgggcc atggaattgg
     1201 gagagcgcat ctttctgtct accggctcca aagaattacc aacttttttg agtgcccctt
     1261 ctgcagcaaa taagcagtgg ttcctgcgca tccttcccga cccggacttg ctggaaagag
     1321 ctgtagcgct gggcattccc cgcagcaata tctgtgccat gcagggacct ttctcccagg
     1381 caattaacga agccctctgg cgggactggc gaatcgactg cgtggtcacc aaagactccg
     1441 gttcaaaagg tggcctttcg gaaaaaataa aggcaacaga aaacctggcc ctacctttaa
     1501 ttgtggtggc gagaccgcca gccgcatcgc cccggaacac tcacaaaagc gtcccccaac
     1561 ttatcgaagc cctgaaccac ctgaatttat aaacgagaga ggagcaatag acgtgccagg
     1621 caaactacca
//
LOCUS       DAOQNZ010001058         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_57919_length_1630_cov_3.032381, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001058 DAOQNZ010000000
VERSION     DAOQNZ010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57919_length_1630_cov_3.032381"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKW73_16320"
     CDS             complement(<1..327)
                     /locus_tag="PKW73_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74917.1"
                     /translation="MHNDDLLTDENESRSASDRQLPATTGNTALKSGQNYESTAPQSD
                     EMALLPGQVLISVMEKVRTTMFLVGWFYSALGFLLAVGAIILLAPGLFWLTDKSSPIL
                     INFAQVA"
     gene            complement(334..>1630)
                     /locus_tag="PKW73_16325"
     CDS             complement(334..>1630)
                     /locus_tag="PKW73_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA74918.1"
                     /translation="LPANNKLYNSNICESPKKNKTMATVSLYDCYIYARTRDEIMLET
                     KATSQGQTGKFTEEQLSWLKKAKDFARRVSLSEVMASDRENVFRRDLFEAACKEGMGA
                     LPFPPTYGGSGGDYLSFALLNEEFGRTCMPVMSSLGVHVLCQEPIYRFGTEEQKQKYL
                     VPAASGKYLGAFGLTEPNAGSDTAAIETTAKLEGDHYILNGTKTFITSGASADFYIVM
                     AKLLEPGAESKKGNITSFIVERGFEGFAIGQKFDMLGMRGYSTCEIVFNDCRVPQANL
                     LGAHGEGRKVALSSLAKGRITIAAQSIGWAQGALDACTETLRRYYLDGLTSADAAGQA
                     LALGNLAVLTESARVLTYQAARLVDAGEASVTLAAMAKTQATDVAMRVATEAISLIGI
                     DGLKPDILIERIFRDAKAGQIYEGTNQIQRMLIARDLLK"
BASE COUNT          360 a          423 c          456 g          391 t
ORIGIN      
        1 agccacctgg gcaaagttga tcaatatggg gctggacttg tcggtgagcc agaaaagacc
       61 aggcgcaagc aaaatgatcg ctcccacggc cagaagaaag ccgagggcag agtaaaacca
      121 accgaccaaa aacatcgtag tgcgtacttt ttccatgacg cttatcagta cttgcccggg
      181 caagagtgcc atttcatcag actgaggagc ggtcgattca tagttctgac cgcttttgag
      241 agcagtatta ccggtagtgg cgggtaattg ccggtcggaa gcagagcggc tctcattctc
      301 atctgttagt agatcgtcgt tatgcatgtg tacctacttt agaaggtctc tggcaatgag
      361 catgcgctga atttgattgg tgccttcgta aatttgaccg gctttggcat ctcggaaaat
      421 tctttctatg agaatgtcgg gcttcagacc gtctatgccg atgagagaaa tggcctcggt
      481 agccacgcgc atggccacat cggtggcctg ggtcttggcc atggccgcca gggtgacgga
      541 ggcttcgccg gcatccacca ggcgggccgc ctgataggtg agcacccggg ccgactccgt
      601 caatacggcc aggttgccca gggccagtgc ctgaccggct gcatcggcgc tggtcagacc
      661 gtccaggtaa taacgtctga gggtttcggt gcaggcgtcc agcgcaccct gagcccagcc
      721 tatggactgg gcggcgattg taattctgcc cttagccaga ctggagagtg ctacttttcg
      781 tccctcaccg tgggcgccca gcaaattggc ctggggcacc cggcagtcat tgaaaactat
      841 ctcacaggtg gaatagccgc gcatgccgag catatcgaac ttctgaccga tggcaaagcc
      901 ttcgaagccg cgctctacta tgaaggaagt gatattgcct ttcttacttt cggcgccggg
      961 ctccaggagt ttggccataa caatataaaa atcagcggag gcacctgaag tgatgaaggt
     1021 tttggttccg ttcaggatgt agtggtcgcc ttccagttta gccgtggtct cgatggcggc
     1081 agtgtccgag ccggcattag gctcggtcaa accgaaggca cccaggtatt tgccgctggc
     1141 tgccggcacc aggtattttt gcttttgttc ttccgtacca aagcgataaa tgggctcttg
     1201 acagagcaca tgcacgccca gggaactcat caccggcatg caagtgcggc cgaattcttc
     1261 gttcaagagg gcgaaactca agtaatcgcc gccggagccg ccgtaggtgg gaggaaaagg
     1321 cagggcgccc atcccttctt tgcaagctgc ttcaaagagg tcgcggcgga atacgttttc
     1381 ccggtcgctg gccatgactt ccgacaagct gacgcgcctg gcaaaatctt tagctttctt
     1441 cagccaggat agctgttctt ccgtgaattt gccggtctga ccctggctgg tggcttttgt
     1501 ctcaagcatg atctcatccc ttgtacgtgc ataaatatag cagtcataga gagaaacagt
     1561 cgccatagtc ttatttttct taggactttc acagatattg ctgttataca atttattatt
     1621 tgctggtaac
//
LOCUS       DAOQNZ010001059         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58145_length_1627_cov_3.255089, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001059 DAOQNZ010000000
VERSION     DAOQNZ010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58145_length_1627_cov_3.255089"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKW73_16330"
     CDS             complement(<1..280)
                     /locus_tag="PKW73_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74919.1"
                     /translation="MSTVTSSTNSSLSSLSSPGTAPAQSQALNLKTGTGEPPSPSIPV
                     PVILDEKLLIEIVRASAIVSSETLEQVIDLARVSQRTILDVLFASGFIA"
     gene            complement(597..>1627)
                     /locus_tag="PKW73_16335"
     CDS             complement(597..>1627)
                     /locus_tag="PKW73_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74920.1"
                     /translation="GFCLEPEKAAESTSVGSGTVLKGEVSYCVPKGTGIKLKLASVPT
                     NGMRLLDRDLDGNLLPAHVGDVISARTTEDIFVEDNKVIPAGTMFRGKVSNVIPPRRL
                     QRPGWLEISFNELELPNGTKFAFRAEADNFQKSTWKSKGRALGKFTAHAAGGAIVGAL
                     AAYKIFGAQNTAAMHGYNIAGGAAAGALIAAGYAMAQKGKKATLEPGDDLNLSIDTDL
                     LMPALQEPTKKVAFDNLPGLSIKVEKSKVVKDGLGGNFLRLDVEIENHTEQRLNAIDL
                     FARDTNGNKYPTSMGPDEESGFGFILEPYSSLRTRLYFNSNYPKLQHELIWLDHKSRR
                     ICFRQKIQ"
BASE COUNT          388 a          403 c          456 g          380 t
ORIGIN      
        1 tggcaataaa accggaggcg aaaagcacgt ccaggatagt gcgctggctg acccgggcca
       61 gatcaatgac ctgctcaagt gtctcactgg aaactatggc agaggcccga actatctcga
      121 tcagaagctt ctcatccagt attaccggta cgggaatgga tggagagggc ggttctcctg
      181 tgccggtctt gaggttcaaa gcctggctct gagccggggc cgtccctggt gaggaaagtg
      241 atgaaagcga ggaatttgtg gagctggtaa ctgtagacat tggatatact cgcaccagaa
      301 cccgcctgcg gcaaagttct atagtacctg aagtacatta ggtacacagg ctttggaaaa
      361 ggaatttata gaagcaggta ctgaaaggag ctggagaaac ttctcagaag ccctgaatta
      421 tgacccgctg aatgaccgac tgaacaaaag gacggaggga attactgtga ggctcgggcc
      481 cgtggtaaat gtgtgagtta acgtccacat tataggttta tcgcaccttg atatgtcaat
      541 ggcgctctac gtagaaacac taagagcaga ccgaaaattt tatggaaggc tctaaattac
      601 tgaattttct gccggaaaca gatgcgtctc gatttgtgat ctagccagat cagttcatgt
      661 tgcaacttgg gatagttgga attgaaatag agcctggtgc gaagggatga ataaggttcc
      721 agaataaagc cgaagcctga ttcttcatcg ggtcccatgg aagtcgggta cttattgccg
      781 ttggtgtcgc gagcaaacag gtcaatggca ttcagcctct gctccgtatg gttttctatt
      841 tccacgtcca gacgcaagaa attgccgccc agcccatcct tgaccacctt agacttttcc
      901 accttgatgg aaagccccgg taaattgtcg aaagcaacct ttttagtagg ttcctgcaga
      961 gccggcatca ataagtcggt gtcgatggag agattcaagt cgtctccggg ctcgagagtg
     1021 gcttttttgc ctttctgagc catggcataa ccggcggcga tgagggcccc ggccgcggcg
     1081 ccaccggcta tgttgtagcc gtgcatggca gccgtattct gagcgccaaa aatcttgtaa
     1141 gcggccagag cgccgacgat ggcaccaccg gcagcatggg cggtaaattt gcccagggct
     1201 cgccctttag acttccaggt ggatttctga aaattatcgg cctcggcccg gaaggcgaac
     1261 ttagtgccgt tgggcagttc cagttcgtta aaagaaattt caagccagcc cggtctctgc
     1321 agtcgccggg gtggaattac attggagacc ttgccccgga acattgtgcc ggcaggaatt
     1381 accttgttgt cctccacgaa tatgtcttcc gtggtgcggg ctgaaatcac atcgcccaca
     1441 tgggcgggca acagattgcc gtccagatcc cggtccaaca ggcgcatgcc gttggtgggg
     1501 acgctggcca gtttgagctt gatgcccgtg cctttaggca cgcaatagct cacttcccct
     1561 ttcaagacag tgccgcttcc cactgatgtg gactcggctg ccttttcggg ctccaggcag
     1621 aagcccg
//
LOCUS       DAOQNZ010001060         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58203_length_1626_cov_3.977085, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001060 DAOQNZ010000000
VERSION     DAOQNZ010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58203_length_1626_cov_3.977085"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKW73_16340"
     CDS             complement(<1..743)
                     /locus_tag="PKW73_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74921.1"
                     /translation="MFRIDFDRLQAGRKPFRLSFSILPVLALSLLFTVSQARGQSAGI
                     YQQPNYWCDMRYQKTGDGDYRNPMQVRYNDNMQPFTWRPMSEDIYAEPNPPTPTPADI
                     INRSGGAGQYQSGSMLSSGGSAQGSSRSLSSAPPASQSVTGAVVAPSSVSRAASGSAF
                     AGGSTAFGAASMGGGSSIAGGSSVAGASSTAGASSTAGVGAAGLPVTTVSGAVAGLQS
                     GNSADSASTSTAATEPLSQYVMTDEAAPD"
     gene            complement(942..>1626)
                     /locus_tag="PKW73_16345"
     CDS             complement(942..>1626)
                     /locus_tag="PKW73_16345"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNA74922.1"
                     /translation="KSAPEVQKAAALAMGSVLEASSMEGAEEPTGYLARALSESTDWE
                     VRLACANALSKGRMSPTSKAIPVLEKALHDPVEKVRLGALQALMAHADTCPQLIKYVG
                     LGLKESDYSTSGNAVNIAAALGPRAKELAPAVAELARRKVGGAANALFKIGAQYAPDG
                     MSVLTGALDSAESKNLWLNRGPALDNLETMDKSTAMQALPTLHRVLEQCNTSDRERCQ
                     KLINYLESP"
BASE COUNT          387 a          474 c          446 g          319 t
ORIGIN      
        1 tagtccggag ccgcttcatc ggtcataaca tactggctga gaggctcggt ggcagcagta
       61 gaggtagagg cagaatctgc cgagttaccg ctctgcaaac cggctacggc accggagacg
      121 gtggtcaccg gcaagcctgc cgctcctaca ccggcagtag atgaggcacc ggcagtagac
      181 gaggcaccgg cgacagatga gcccccagcg atagatgagc ccccgcccat agaagccgca
      241 ccaaaagcgg tactaccacc ggcgaatgca gagccggagg ccgctcttga tacggaagac
      301 ggcgccacca ctgctcctgt taccgattgc gatgccggcg gcgcgctgga aagcgagcga
      361 ctgctcccct gggcactgcc accactggag agcatggagc cgctctgata ctgcccggcg
      421 ccaccggatc tattgatgat atctgccggc gtcggcgtgg gtggattagg ctcggcgtaa
      481 atatcctcac tcatgggacg ccaggtgaag ggctgcatat tgtcgttgta gcgcacctgc
      541 atggggtttc tgtagtcccc gtcgccggtt ttctggtagc gcatgtcgca ccagtaattg
      601 ggctgttgat aaatgccggc gctctgtccc cgggcttgag agactgtaaa taaaagggac
      661 agagccagaa ccggcaagat tgaaaaacta agacgaaaag gtttcctccc ggcctgaagc
      721 cgatcaaaat caattctgaa catggcgcgc tccgcaacga atcactcaga caatcaagca
      781 ccgccatgcg cccagagaac gggaagggcc gaacttgctt gtctgatttt ttagtaagtg
      841 aacagcaaag aaaatagaat tgggcaaacg ccaggacaat aaattaagcc ccaagcgccc
      901 gcttgtcaag gcaaatttta tgaaactttt ggaagcttcc cctagggcga ctcaagataa
      961 ttgatgagct tttggcaacg ctctctatcg cttgtattgc actgctcaag cacccggtgc
     1021 aaggtcggca gcgcctgcat ggccgttgac ttatccatag tctccagatt atccagggcc
     1081 ggtcctctat tcaaccagag gttcttggat tcagcactat ccagcgcgcc cgtcaacacc
     1141 gacatgccat ccggtgcgta ctgggcacca attttaaata aagcattggc agcaccaccg
     1201 accttgcgcc tggccagttc ggccacggcc ggagccagtt ctttagcacg aggaccaagg
     1261 gcggcagcaa tgttcacggc attgccgcta gtagaataat cagactcctt gagacccaag
     1321 cccacatact tgatgagctg agggcaggtg tctgcatggg ccataagagc ctgaagggca
     1381 cccagccgaa ccttctccac cggatcatgc aaagcttttt ccagcactgg aatcgctttg
     1441 cttgtgggcg acatgcgccc tttggaaagg gcattggcac aggcaagacg cacttcccaa
     1501 tcagtactct cggaaagagc ccgggccaga tagccagtgg gttcttccgc cccctccata
     1561 gacgaagctt ccaacaccga gcccatggcc agtgccgccg ccttttgcac ctcaggggca
     1621 ctctta
//
LOCUS       DAOQNZ010001061         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58204_length_1626_cov_3.965627, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001061 DAOQNZ010000000
VERSION     DAOQNZ010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58204_length_1626_cov_3.965627"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKW73_16350"
     CDS             complement(<1..229)
                     /locus_tag="PKW73_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74923.1"
                     /translation="MSKISISVLVFFLGLAGYLGFTGFTRLESDINEVKKMAASVAWP
                     TADAVITSSSVTTGKASKGRQTFKPKVIYSYS"
     gene            complement(340..>1626)
                     /locus_tag="PKW73_16355"
     CDS             complement(340..>1626)
                     /locus_tag="PKW73_16355"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74924.1"
                     /translation="LVSYWCPDKGYEWFGENPGHEALTAFGLLHFTDMAQVREVDKNM
                     IASTRAWLFKQRDGKGGFSRKRRALHTWIEDKDCSNAYILWAMLETGQPASDLAPEIS
                     SLKSASAVSQNNYVVALAANALHLSGNKGEAKTLMAKLAARQKPDGSIADVKSSIVGS
                     DGEALAVEGAALATLAWMREPEYAGNVEKSIKFLADSCKAGRYGSTQSTVLALRAIVH
                     YDQLRARPKAPGKVRVFVDGQSVGDWAAFTPATEGAITLPDLSELLPPGEHKLELRMD
                     GGSAMPYSMAVKYNAVTPQTSDKCKLDMNVALAQTKLTEGAATEANVVITNKEKEVLP
                     TPMAIVGLPGGLEPRHDQLKELVKKGKIDAYEVLGREVVLYWRTLPEQARVEIPISLI
                     AAVPGSYTGPASRSYLYYCDEHKQWVDGLKVEIAAK"
BASE COUNT          339 a          456 c          451 g          380 t
ORIGIN      
        1 ccgaataaga gtaaataacc tttggtttga aggtctggcg ccctttgctg gcttttccag
       61 tagtcacact gctacttgta atgacggcat cggcagtggg ccaggccaca ctggctgcca
      121 ttttcttgac ttcatttatg tccgattcca ggcgggtgaa gccggtaaag ccaaggtaac
      181 cggctaagcc gagaaagaag acaagcactg agattgatat cttcgacata agggaaatat
      241 tagcatactg ccgggaagca atagaacgcc gcagagcaat agtttcgcct tatcgccggc
      301 gctattgctc tgcggcattc cccaaatcat tgcaccaact tactttgcag caatctccac
      361 tttcagaccg tccacccatt gcttgtgctc atcgcagtaa tagagatagc tacggctggc
      421 aggaccagtg tacgaacccg gaacggcggc aatcaagctg atgggaattt ccacccgcgc
      481 ttgctccggc agggtgcgcc agtagagcac gacttcgcga ccgagcactt cataggcgtc
      541 gatcttgccc ttcttgacca gttctttgag ctgatcatgg cggggttcga gtccgcccgg
      601 aagacctacg atagccatcg gggtgggcaa gacttccttc tctttgttgg tgatgacgac
      661 gttcgcttcc gtggcggcgc cttcagtcaa cttggtctga gcgagagcta cattcatgtc
      721 cagcttgcac ttgtcggagg tctggggagt gacggcattg tacttgaccg ccatggaata
      781 tggcatggca gaaccaccgt ccatacgcag ctccagtttg tgctcgccag gcggaagtag
      841 ttcggacagg tcgggcaaag tgatagcacc ctcggtggca ggtgtgaagg ccgcccagtc
      901 gcctacggat tgaccatcta caaagacccg cacttttcca ggcgccttcg gtctggctct
      961 cagttggtcg tagtgcacaa tggctctcaa ggccagcacc gtcgactgtg ttgaaccgta
     1021 acgtccggcc ttgcaagaat cagccaggaa cttgatggac ttctccacat tgcctgcata
     1081 ctcaggttcg cgcatccagg caagggtagc cagcgctgcc ccttccaccg ccagggcttc
     1141 gccgtcgctg cccacaatcg agcttttcac atcggcgata ctaccgtcgg gcttctgcct
     1201 ggcggcaagt ttcgccatga gcgtcttcgc ttcacccttg ttgccggaaa gatgaagggc
     1261 attggccgcc agtgccacca cataattatt ctggctgacg gcagaggcac tcttgaggct
     1321 gcttatctcg ggagccaggt cgcttgccgg ttggccggtt tcaagcatgg cccagagtat
     1381 ataggcatta gagcagtctt tgtcttcgat ccaggtgtgc agggcgcggc gcttgcgtga
     1441 gaagccgccc ttgccgtctc tttgcttgaa gagccaggcg cgggtggagg cgatcatgtt
     1501 tttatcaacc tcgcgtacct gggccatgtc ggtgaagtgc aagagtccaa aggcagttag
     1561 agcctcgtgc ccgggatttt caccgaacca ctcatagcct ttgtcggggc accaataact
     1621 taccag
//
LOCUS       DAOQNZ010001062         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58231_length_1626_cov_2.977721, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001062 DAOQNZ010000000
VERSION     DAOQNZ010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58231_length_1626_cov_2.977721"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKW73_16360"
     CDS             complement(<1..710)
                     /locus_tag="PKW73_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74925.1"
                     /translation="MPANHKVKLCMAALPLLFYLFSLPPLVCAQTKNTATVSVAAGPL
                     EAERRALLEKLQAAKIRGIEIESFLSAFAELEGMAKKRCVREDIQAKLNQISSDLTDR
                     LENRKKPVIEVPATKPSAIVKTQTLTLSQARQFALKLVNQDRARYHLAPLRLDTIASR
                     AGQIHTDEMAKFNYCSHWDTSGRKPYQRYTEAGGTHESGENLAIRSGMTETPGPNLFT
                     TDEIETLHALFMIEKPPE"
     gene            1035..>1626
                     /locus_tag="PKW73_16365"
     CDS             1035..>1626
                     /locus_tag="PKW73_16365"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNA74926.1"
                     /translation="MKTIIEPFRTKVVEAIKFTSRAEREEILKKAGYNLFQIKAEDVM
                     VDFLTDSGTSAMSSEQWAALMRGDESYAGCSSYFRFEQTVQEIFGFPIVIPVHQGRAA
                     ERLLFGELVKPGHVVPSNTHFDTTRANIEFLGGEAIDLPCQEAKDSFSQFPFKGNMDI
                     EALRALLDARGESVPFIMVTVTNNAVGGQPVSVENVR"
BASE COUNT          345 a          374 c          469 g          438 t
ORIGIN      
        1 tcttccggtg gcttttcaat cataaagaga gcatgcaagg tctcaatctc atcggtggta
       61 aatagatttg gtcccggtgt ctcggtcata ccggagcgta tggcaagatt ctcaccgctt
      121 tcatgggtgc cgcctgcttc ggtatagcgc tggtagggtt ttcgcccgga agtgtcccag
      181 tgcgaacagt agttgaattt tgccatctcg tcagtgtgta tttgtccggc gcggctggcg
      241 atggtatcaa ggcgtagggg tgccaggtga tatctggcgc ggtcctggtt tactagcttg
      301 agagcaaatt gacgtgcctg gctaagagtc agggtttgtg ttttgactat ggctgagggc
      361 ttcgtggccg gtacttctat gactggtttc ttgcggtttt ccaggcggtc cgtcaagtct
      421 gatgaaattt ggttgagctt ggcttgaatg tcttcgcgca cgcatcgttt cttggccatg
      481 ccttccagtt ctgcgaaagc cgacaggaag ctctcgattt caatgcccct tatctttgcg
      541 gcttgaagct tttccaatag agcccggcgt tccgcctcaa ggggaccggc ggctactgag
      601 acagtagcag tgttcttggt ttgcgcgcat accagtgggg gtaggctgaa caggtaaaac
      661 agcagcggca gcgctgccat acagagtttt accttgtgat ttgcaggcaa gggcgttggc
      721 accgatttaa ttccgtcaag attaacttgc gaagatagac atccattttg attctacaga
      781 agtttacgtg agcagggcgc ggagttgatc gcacccgctg gaagtcaagc gtgccaccgg
      841 tatccttttg gaatccaccc tgggggtagt ggcaataagc cggtcggatg atagaagctg
      901 cgctcgaaat tgctattctt atcgcatcaa agacgcccac cccaagcatg atcctcttaa
      961 ggatttgaat ttcggagtaa gggcgtcttt attttttgcc gtttgtctgt tcggtttgca
     1021 tcagaggttt tgacgtgaaa acaattattg agccttttcg caccaaggta gtggaagcca
     1081 ttaagtttac gtccagggct gagcgagaag aaatactcaa gaaagccgga tacaacctct
     1141 ttcaaattaa agccgaagat gtgatggtgg atttcctaac cgacagcggc acttctgcca
     1201 tgtcgagcga gcagtgggcg gcccttatgc gcggcgatga gtcttatgcc ggttgctcca
     1261 gctattttcg ctttgaacag actgtgcagg agattttcgg cttcccaatc gtgattccgg
     1321 tgcaccaggg tagagccgcc gagcgtctgc tctttggcga actggttaag cctgggcatg
     1381 tggtgccgag caacacgcat tttgatacaa caagagccaa tatcgagttc cttggcggtg
     1441 aggctattga tctgccctgc caggaagcga aagattcttt ctcccagttc ccgttcaaag
     1501 ggaacatgga tattgaagcg ctaagggcgt tgcttgatgc taggggcgag tcggtgccct
     1561 tcattatggt gacggtcaca aacaatgccg tgggcggtca gccggtctcg gtggagaatg
     1621 tcagag
//
LOCUS       DAOQNZ010001063         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58420_length_1623_cov_3.764031, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001063 DAOQNZ010000000
VERSION     DAOQNZ010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58420_length_1623_cov_3.764031"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..292
                     /locus_tag="PKW73_16370"
     CDS             101..292
                     /locus_tag="PKW73_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74927.1"
                     /translation="MSQANTSHILEEKLKRLSDPSDIAATLKAYLEIERQNHSEREAV
                     FSSPLTSARVICLDRLKAV"
     gene            361..660
                     /locus_tag="PKW73_16375"
     CDS             361..660
                     /locus_tag="PKW73_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNA74928.1"
                     /translation="MKTKEAVSALQALAQETRLEIFRLLVRKGTGGMAAGELSGHFAI
                     PAATMSFHLKELSQAGLLVSRRESRSIIYSANYEHMQLLLGFLLENCCSDNGGKC"
     gene            660..1154
                     /locus_tag="PKW73_16380"
     CDS             660..1154
                     /locus_tag="PKW73_16380"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HNA74929.1"
                     /translation="MKRFHVHLAVADLEESIKFYSTFFNSPPAVRKEDYAKWMLDDPR
                     LNFAVSTRGRMPGLDHLGLQVEDGDELKQVSLRLKEAGKPVLEQEQTTCCYAQSDKTW
                     VMDPQGVPWETFYTTASATTYGEDAELQMPAKSACCAPASEIAAVSVGIDCSKDGGRK
                     DGER"
     gene            1151..1567
                     /locus_tag="PKW73_16385"
     CDS             1151..1567
                     /locus_tag="PKW73_16385"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNA74930.1"
                     /translation="MKVIFACVHNAGRSQMAAAFFNHFADPERALALSAGTAPGDGVH
                     ACVLTSMQAVGLDLSSARPQLLTEELAKDADMLITMGCGEACPHVPGLKRDDWPLSDP
                     KGKSEQEVNLIRDEIKERVLALLKSYDLLKVNVSAV"
BASE COUNT          387 a          386 c          464 g          386 t
ORIGIN      
        1 ccagcagggg tgcgaaggct aagaccgcca aacggaagcc gaagaaaggc aagaaagtaa
       61 aagccaagtc tgtgtacaaa gccaaaggca ggaagtaatc atgtctcaag caaatacctc
      121 gcacatcctg gaagagaaac tgaagcgtct ttccgatccc tctgatattg ctgccaccct
      181 caaggcttac ctggaaattg agaggcaaaa tcacagtgaa cgtgaggcag ttttctcttc
      241 tcctcttaca tcggcgcgcg tcatttgcct tgaccgactg aaagcagtct gattttcatt
      301 ttagtcagtg ccgggagacc ggcacttgac tctgtctgtc atttcgatta tattggaagt
      361 atgaaaacga aagaagccgt cagtgccctt caggctctgg cgcaagagac aaggttggaa
      421 atattcaggc tgctggttcg caaaggtact ggcggcatgg ccgccggaga gttgagcgga
      481 catttcgcta ttccggcggc cacaatgtcc tttcatttga aagagctgag ccaggccggc
      541 ttacttgtct cccgtcgcga aagtcgttcc attatttatt ccgctaacta tgagcatatg
      601 cagttgctct tgggcttttt gctggagaac tgctgctctg acaacggggg caagtgctga
      661 tgaagagatt tcatgtccat ctggccgtgg ctgatttgga agagtcgatc aagttttact
      721 cgactttttt caactcaccg cctgcggtcc ggaaggaaga ttatgccaag tggatgctcg
      781 atgatccgcg cctcaacttt gctgtttcta caagagggcg gatgcccggt ttggatcacc
      841 tgggtttgca ggtggaagac ggggatgaac tgaagcaggt ttccctgcgg ctgaaagaag
      901 ccggcaaacc ggttctggaa caggaacaaa ccacctgttg ctacgctcaa tccgacaaga
      961 cctgggtaat ggatccccag ggtgtgccct gggaaacctt ctacaccacc gcttctgcca
     1021 ctacatatgg tgaagatgcc gaactgcaaa tgccggcgaa gtcggcttgt tgcgctccgg
     1081 catcagagat agcagcagtt tcagtcggca ttgattgcag taaggatggc gggcgaaagg
     1141 atggtgagag gtgaaagtga tctttgcctg cgtgcataac gcgggccgct cgcagatggc
     1201 tgctgctttc tttaatcatt tcgccgatcc tgaaagagcc ctggcccttt cggccggcac
     1261 cgcccccggt gacggtgtcc atgcctgcgt gttaacgtcc atgcaggcgg tggggctaga
     1321 tctctcgtct gccagaccgc agcttctgac ggaagagttg gctaaggatg cggacatgct
     1381 catcactatg gggtgtggcg aggcctgtcc ccatgtgccc gggctcaagc gcgatgattg
     1441 gccactatct gatccgaaag gtaagtctga gcaagaggtg aatttgattc gagatgagat
     1501 taaggagcgg gtgctggctt tattgaagag ctatgatctg ctgaaagtaa atgtgagcgc
     1561 ggtctagaaa caggagaaaa ggtatcgtga aaatcaaagt gtttgagccg cccatgtgtt
     1621 gtt
//
LOCUS       DAOQNZ010001064         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58437_length_1623_cov_3.036990, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001064 DAOQNZ010000000
VERSION     DAOQNZ010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58437_length_1623_cov_3.036990"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1623)
                     /locus_tag="PKW73_16390"
     CDS             complement(<1..>1623)
                     /locus_tag="PKW73_16390"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA74931.1"
                     /translation="ATRSEPARGLPTTSGPAASEPTKSRIETQAGGRTETRRGGQGEA
                     KGSATVISAGAGAAFASAPKTGVRKSGAGATAERPAFVDMRGKPISEIPVQFVKGVGP
                     RIANLLAKLDISTVEKLLCHYPRKHLDFLNREHIRDLVIGEEVSIFGTIRSVSAYQAK
                     RGNVSILSAIISDGTGSIIVSRFVGGKSNKYLLDRYKEQFPKGAQVLASGVVERDDYS
                     GKLKLKNAEIEILSLSGGEQLSSGYSDEDADGSMPESLHAGRLVPVYPLTEGLSLRYL
                     RNIIHNALSSYGDGIDELLPDELRETYSLMGLKQSFFNIHFPQTPEDKDEARRRLVFD
                     ELFGIQLQMAHRRYQYEQREDALSIKYEPAADGYVNRLRQSLPFTLTGAQERVFGEIA
                     RDLASNKPMHRLVQGDVGSGKTVVALMAFMVAIENGFQGAMMAPTEILAEQHYRQFQR
                     LLAPLGLRASLVLGKHGVKERRAARQEILSGQSHIVVGTHAILEDDVEFQNLGLIIID
                     EQHRFGVKQRARLKAKSQNPELLTMTATPIPRT"
BASE COUNT          364 a          460 c          414 g          385 t
ORIGIN      
        1 cgtccgtggt atcggtgtgg cagtcatggt gagaagctca ggattctgac tcttggcttt
       61 cagcctggct ctttgcttaa cgccgaaacg atgctgttca tcgatgatga tcaagcccag
      121 attctgaaac tccacatcat cttccagaat ggcgtgggtt cccaccacaa tatgactttg
      181 cccggagaga atctcttgcc tggcggcacg ccgttccttg acgccgtgct tgcccagcac
      241 cagagaagca cgcaagccta aaggcgccag cagacgctga aactgccggt aatgctgctc
      301 ggcaagtatt tcggtaggag ccatcatggc accctgaaag ccgttttcta tggccaccat
      361 gaaagccata agggccacca cggtctttcc ggaaccgaca tcgccctgca ccaggcggtg
      421 catgggtttg ttggaagcaa gatctctggc tatctcacca aaaacacgct cctgggcgcc
      481 ggtaagagta aacggcaggg actgccgcaa ccgattgacg taaccgtcgg cagccggctc
      541 atacttgatt gatagcgcat cttctcgttg ctcatactga tagcggcgat gagccatctg
      601 aagctgtatg ccgaagagtt cgtcgaaaac caggcgccgc ctcgcttcat ccttgtcttc
      661 cggcgtctgc ggaaagtgta tattgaaaaa ggactgcttc aagcccatca aactataggt
      721 ttcccgaagc tcgtccggca gcagttcatc gataccatca ccataactgc tcaaagcatt
      781 gtgaatgata tttctaaggt agcgaagcga aagtccctca gtaagcggat aaaccggtac
      841 aagtcgcccg gcatgaagac tttcaggcat tgatccatcc gcatcttcat cgctgtagcc
      901 gctggacaat tgctccccgc cggagagact caatatttca atctcggcat tcttgagttt
      961 caacttacct gagtagtcgt ctcgctcaac cacaccggat gccagaacct gagcaccttt
     1021 tggaaattgc tctttgtagc ggtcaagcaa atacttattg gactttccgc cgacaaagcg
     1081 actgacaatg atactgccgg tgccatcaga gattatggcg ctcagtatgg atacattgcc
     1141 gcgcttagcc tgataagcgc tcaccgagcg aattgtgccg aagatactca cttcctcgcc
     1201 gataaccaga tcgcgaatat gctctcggtt caaaaagtcg agatgcttgc gagggtagtg
     1261 gcagagcagt ttctctaccg tgctgatatc cagtttggca agcagattgg caatacgcgg
     1321 accgacgccc ttgacgaact gcactggtat ctcggaaatc ggcttgcctc gcatatcaac
     1381 aaatgccggg cgctcggcag tagcaccggc gccactctta cgaacaccgg tcttgggtgc
     1441 cgatgcaaaa gcggctccgg caccggcact tatgacggtg gcagagccct tagcttcgcc
     1501 ttgaccgcct cttcgagttt ctgtgcgacc acctgcctgg gtttctattc ggcttttagt
     1561 tggctcactt gctgccggtc cgcttgttgt cggcaggcct ctggccggct cgcttcttgt
     1621 ggc
//
LOCUS       DAOQNZ010001065         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58657_length_1620_cov_4.123962, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001065 DAOQNZ010000000
VERSION     DAOQNZ010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58657_length_1620_cov_4.123962"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1620)
                     /locus_tag="PKW73_16395"
     CDS             complement(<1..>1620)
                     /locus_tag="PKW73_16395"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNA74932.1"
                     /translation="LEANQTALDMVGVDPATLRNKFFWDTPWWQHSPQEQELLKQAIK
                     DAASGKFVRFQTNHIGRGGETITIDFSIKPVLDDSGKVLYLLPEGRDMTEQKRAQEST
                     TASEQHLKALLTCLAEGVYQIDLEGNLVYMNGAAERILQYQENELLGKNMHNIIHSSS
                     DAVPSQAPFPQLSTGQREQPGGDFKLLSVVERGEELRISEDWFRRKDGTCVPVEYVGS
                     PLMQNGEVKGAVIAFQDITTRKEAEKRVSEFYSTVSHELRTPLTSIRGAMRLLEAGKG
                     GELTERGKHLTTMGRIECDRLVRLINDMLDIRKMEAGKLDLRYAEFDAVDAARQAISN
                     LQAYADEHRVSITLKPAPVIMLNADRDRFTQILTNLVSNAVRFSPLDSEVKVSLDNLT
                     GEQTLKVSIQDSGPGIKQTDQRKLFKVFQQLDATDARRKGGTGLGLAICKSLVEQHGG
                     RIGLNSAEGEGATFWFTLPIYGKQQPEKKQPDEKKQPEKKQQDTAISSPAEHKHHSSR
                     YRKSKGKHERVKALIAENDKFTRNLLELQLRR"
BASE COUNT          304 a          411 c          433 g          472 t
ORIGIN      
        1 acgcctgagt tgcagttcga gcaagttgcg cgtgaacttg tcgttctcgg caatgagagc
       61 ctttactctt tcatgtttgc ctttggattt gcgatagcgt gaggagtgat gtttgtgctc
      121 cgccggcgaa ctgatggcag tgtcttgctg tttcttctca ggctgtttct tctcgtcagg
      181 ctgtttcttc tcaggctgct gcttaccgta tattggtagg gtaaaccaaa atgtggcgcc
      241 ttctccttct gcactattca gtccgattct gccgccgtgt tgttccacca gtgatttaca
      301 aatagccagg ccaagccctg tgccaccctt cctgcgagca tcggtggcat caagctgttg
      361 aaacaccttg aagagtttgc gctggtccgt ctgtttgatt ccgggtccgc tatcctgtat
      421 ggaaactttt agggtctgtt ccccggtcag gttgtcgagg ctgactttca cttcggagtc
      481 cagaggactg aagcgtaccg cattggatac gagattggtc aatatttgag taaatctgtc
      541 ccggtcggcg tttagcataa tcaccggtgc cggctttaaa gttatggaga cccggtgctc
      601 atcggcgtag gcctgcaggt tgctgatggc ctgccgggcc gcatctacgg cgtcaaattc
      661 agcatagcgc aggtcgagtt ttcctgcttc catttttcgt atgtcgagca tgtcgtttat
      721 gagtcgcacc agtcggtcgc attcgatacg acccatggtg gttaggtgct tgccccgctc
      781 agtcaattct ccgccctttc ctgcctccag aaggcgcatg gcaccgcgaa ttgatgttag
      841 cggggtgcga agttcatggg agactgttga gtagaattcg ctgacgcgtt tctccgcttc
      901 tttgcgggta gtaatatcct ggaaggcaat cactgctcct ttcacttcac cattttgcat
      961 gagcggtgaa cctacatatt cgaccggaac gcaagtgccg tctttgcggc ggaaccagtc
     1021 ttctgaaata cgcagttctt ctcctctttc aaccacgctc aatagtttga agtcaccgcc
     1081 gggttgctct ctttgaccgg tgcttaactg agggaagggg gcctgggaag gaactgcatc
     1141 gctactgctg tgaatgatgt tgtgcatgtt tttgcccagc agttcgttct cttgatattg
     1201 cagtatgcgc tcggccgcac cattcatgta gactagattg ccttccaaat caatttgata
     1261 gaccccttcc gccaggcagg tgagcaaggc cttgagatgt tgttcactag ccgtggtgct
     1321 ttcctgggcg cgtttttgtt cggtcatatc gcgaccttcc ggaagcaggt aaagcacttt
     1381 gccggagtcg tcgagcacag gcttgataga aaaatctata gtgattgtct cgccgcctct
     1441 gcctatatga ttcgtctgga agcgcacgaa tttgccggaa gcggcgtcct ttatagcttg
     1501 cttaagcagt tcttgctcct ggggagagtg ctgccaccaa ggtgtgtccc agaaaaactt
     1561 attgcgtaag gtggctgggt ctactcctac catatcgaga gccgtctggt ttgcttccag
//
LOCUS       DAOQNZ010001066         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58734_length_1619_cov_3.416240, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001066 DAOQNZ010000000
VERSION     DAOQNZ010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58734_length_1619_cov_3.416240"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..847
                     /locus_tag="PKW73_16400"
     CDS             17..847
                     /locus_tag="PKW73_16400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNA74933.1"
                     /translation="MQQLQSNPLVSILFLGDVIGKPGRLVVKKYLQQLKDGAEQGLKP
                     FPDFVVVNVENACHGFGVSRENIQELKECGVDVFSGGNHTFDRKEIFDFIDSEETLLR
                     PANYPEGTAGKGLVVVEKDQVRLAVINLMGRVFMEPLQSPFAVVDKLLNELQEKDENC
                     LILVDVHAEATAEKVALGWYLDGRVQAVVGSHTHVQTADERLLKQGTAYITDAGACGP
                     RDGVIGMAQEGVFRRMIKQLPSRFELAPGPAAACGVLIGVDKASKQAVSIERVRFEED
                     "
     gene            847..>1619
                     /locus_tag="PKW73_16405"
     CDS             847..>1619
                     /locus_tag="PKW73_16405"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNA74934.1"
                     /translation="MACPVDLHLHSHFSDGTWSPEALVRHAVELGMEYIALSDHDTMD
                     GIEEAKQALLALGDTSEAARQLVLLPAIEINCLWQYEGVYHDVHILGYLPDKSARALV
                     EVMDLQRQARLEQLNLSLEKINDSLKGRRAVTFEDVQACAGKGNIGKAHLTEALVRVG
                     AACDLMDAYERFTARTAPCYVQRQSVSPFQALTAIKAAGGFSSIAHPGSETHMPLLLA
                     QLIDAGLEGIEAYHRIHSEAVRKHYVDLAQEKNLLVTGGS"
BASE COUNT          361 a          389 c          483 g          386 t
ORIGIN      
        1 ggtgtaagct ctagtgatgc aacagttaca gtctaaccca ttagtttcca tactcttcct
       61 tggagacgtc atcggcaagc ccggacggct tgtggtgaag aaatacttac aacagctgaa
      121 ggatggcgct gagcagggtc ttaagccctt tcccgatttt gtggtggtga atgtagagaa
      181 cgcctgccac ggtttcggcg tctccaggga aaacattcaa gagctgaaag agtgcggcgt
      241 cgacgtgttt tccggcggca atcatacttt tgatcgcaaa gaaatatttg attttattga
      301 ctcggaagag accctcctgc gcccggccaa ctacccggaa ggcacagccg gcaaggggct
      361 tgtggtggtg gaaaaggatc aggtaaggct tgctgttata aatttaatgg ggcgggtctt
      421 catggaacct ctgcaatcac cttttgccgt ggtcgataag ctgcttaatg aattgcaaga
      481 gaaagatgag aactgtttga ttctggtgga tgtgcacgcc gaggccactg ctgagaaggt
      541 ggctctgggc tggtatctgg acggcagagt gcaggccgtg gtgggcagcc atacccatgt
      601 gcagaccgcc gacgagcggt tactcaagca gggaaccgct tatattaccg acgccggagc
      661 ttgcggacct cgtgatgggg tgattggcat ggcccaggaa ggtgtgttca ggcgcatgat
      721 caagcagctt ccttcccgct tcgagctggc tcccggccct gctgccgctt gcggagttct
      781 aattggtgta gataaagcta gcaagcaggc tgtttccatt gaaagagtgc gctttgagga
      841 agactgatgg cttgccccgt cgatctgcat ttgcacagcc atttttccga cggtacctgg
      901 tcgcctgaag ccctggtcag gcatgcagtt gagcttggta tggagtatat tgccctttcc
      961 gaccatgaca ccatggacgg tattgaagaa gcgaagcagg cgctgcttgc tctgggtgac
     1021 acttcggaag cggcgcggca actggtgctt ttgccggcaa ttgagatcaa ttgtctctgg
     1081 caatatgagg gcgtctatca tgacgtgcat atactcgggt atctaccgga caaaagtgct
     1141 cgggcgctag ttgaagtaat ggacttgcaa aggcaggccc gtttagagca actgaatttg
     1201 agcctggaga agattaacga tagcctgaaa gggcgtaggg ctgtcacttt cgaggatgtg
     1261 caagcctgtg ccggcaaagg caatatcggc aaggctcatt tgacggaagc actggtgcgc
     1321 gtcggtgcgg cttgcgattt gatggatgcc tacgagcgct tcacggccag gacggcacca
     1381 tgttatgtgc agcggcaaag cgtctcgccc tttcaagcac tgaccgccat caaagcggca
     1441 gggggcttca gttccattgc ccatcccggc tctgagaccc atatgccctt attgcttgcc
     1501 caacttatcg acgcaggcct tgagggaatt gaagcctacc atcgtattca ttctgaggct
     1561 gtccggaagc actatgtgga cctggcgcaa gagaagaatt tgcttgtcac aggcggttc
//
LOCUS       DAOQNZ010001067         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58742_length_1619_cov_3.103581, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001067 DAOQNZ010000000
VERSION     DAOQNZ010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58742_length_1619_cov_3.103581"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..564
                     /locus_tag="PKW73_16410"
     CDS             196..564
                     /locus_tag="PKW73_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74935.1"
                     /translation="MVKARALLNFGPLCTNLGGVALLAMLSGAALGYFAGVRSESAPP
                     AVLSRPVSTQIPSIDVHSLSYGAEPVLLGSSQGPTTAAALFPEAASTPVASPPKALPP
                     AYQVVVAAADRMLLKTIVTR"
     gene            737..>1619
                     /locus_tag="PKW73_16415"
     CDS             737..>1619
                     /locus_tag="PKW73_16415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNA74936.1"
                     /translation="MTKSLIRRICLDRNSQVVSGSGVEDSPEDLQADGPLKSDTVDAY
                     LRRLRKCKILPKEEEQSLARAARAGDSRAAKCLAESNLRLVVRIAKHYRNRGLSFEDL
                     IQEGNLGLLRAVQKFDPERGFRFSTYAVWWIRQSIVRAIGNKAKLVKVPPQFEAELKS
                     VSKAAQALRQELGREPSLEEVSQRSGVSRSRIHLVSNLSKEHLSLDAPVWEDQVDAFL
                     EMLKDRSGSEEAVINSLMREEVESLLGILSARERDVIGLRFGLSENGRSLSAVDTGRQ
                     LNLSAERVKRIEARALFK"
BASE COUNT          378 a          363 c          458 g          420 t
ORIGIN      
        1 aatctcagag ctgtccttat gattcaggaa gtaacctgaa aaagcaattt agagctgccc
       61 gaacggtttt ctggatggtg gcgagccctg gcaagtaaat tcagttggtc gagagtctta
      121 ttggagttct tcgagttcaa tagagcctca ggtaagtttc cttcgtaggc tagtagaggg
      181 cggaggtgtt tgtcgatggt aaaggcaaga gctttgctga attttggacc cttatgcacg
      241 aacctgggcg gcgttgccct gcttgcgatg ctcagtggcg cggctctcgg ctattttgcg
      301 ggagttcgtt ctgagtctgc ccctccggca gtgctcagtc ggccggtatc gacgcagatt
      361 ccctcaattg acgtgcatag tctttcgtat ggtgcagaac ctgtactttt agggtcgtct
      421 caaggtccga ctactgccgc tgccttgttt cccgaggccg cctctacacc tgtcgcttcg
      481 ccccctaaag cgctgccgcc agcctatcaa gtcgtggtgg ccgcagcaga taggatgctc
      541 ctcaagacta tcgttactcg ctaagggaga gttttgaaaa aatgattaag gactgaggcg
      601 ccaggctgct cttcgatgta gaaaagtgtt gataggttct atattcactg gcttgtacaa
      661 aagaattaaa atgtagcaag caagcaaaat cgaaacgttt gacggaattg gtttcgctgg
      721 aaagggagtg atatatatga cgaaatcact gattagacgt atttgcttag atcgtaattc
      781 tcaagtagtt tcaggctccg gtgtggaaga ttctcctgaa gatttacagg ccgacggtcc
      841 actaaagtct gatactgtcg atgcttattt gcgtcggctg cgaaagtgca aaatcttacc
      901 caaagaagag gaacagtccc tggctcgcgc tgccagagca ggcgacagta gagctgctaa
      961 gtgtctggct gagagcaatt tgcgattggt ggtgaggatt gccaaacatt atcgcaatcg
     1021 cggcctcagc ttcgaggatc taattcagga gggcaacctc ggtttgttgc gtgccgttca
     1081 gaagtttgat ccggaacgag gcttccgctt ttccacttat gctgtctggt ggatccggca
     1141 gagtatcgtc agagccattg gaaataaggc aaaacttgtg aaagtgccgc ctcagtttga
     1201 agcggagctg aagagcgtga gcaaggccgc ccaggcgctc aggcaggaat tgggacgcga
     1261 gccaagtctc gaggaagtct ctcaacgttc cggggtatct agaagtcgta ttcaccttgt
     1321 atccaatctc tcgaaggagc atctttccct ggatgcacct gtttgggagg atcaagttga
     1381 tgcttttctg gagatgctca aagatcgctc cggaagcgag gaagccgtta tcaactcgct
     1441 catgagagag gaagtcgagt ctttgttggg catccttagt gcgcgcgagc gtgatgtgat
     1501 tggcttgcga ttcggtcttt cagagaacgg tcggtcactc agtgcggttg acactggtag
     1561 gcaattaaac ttgtctgctg agcgcgtcaa acgtatagaa gcaagagcac tgttcaagc
//
LOCUS       DAOQNZ010001068         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58760_length_1619_cov_2.508312, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001068 DAOQNZ010000000
VERSION     DAOQNZ010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58760_length_1619_cov_2.508312"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKW73_16420"
     CDS             <1..475
                     /locus_tag="PKW73_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA74937.1"
                     /translation="LEIGADDYLPKTFSPRELLARLRAVLRRVRRNVEPNLKVSGEGA
                     ESSKDELNLGALIIDLASRSATLSGQSLALTPVEFDLLVVLGQSRGRVKSREQLLNEI
                     RDRNYDIFDRSIDVHISALRRKLGDPPQNPRFIRTVRSAGYMLLNQSESEGGDES"
     gene            465..>1619
                     /locus_tag="PKW73_16425"
     CDS             465..>1619
                     /locus_tag="PKW73_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA74938.1"
                     /translation="MKARTSLYLQVFNLLVLYLLLLCAIVFFVFNSAFGFGWETLIAS
                     PIGERVDTVAGAVSGQLRGRPTSRFNEVLQDFGRIYNVQFYIFDGAGQLAGEKVDLPP
                     AVAGRVQMLLPPGAMGDRGPRPPMALPLPGPFPLRVQGFPEGGAAPPPHGSGRFIVHS
                     GRDGRFWIGTRLPLVGDGAGMSPVLVPGRQFYPPGFPEPFHPAVLLAASDNIWQSSLV
                     IDFAFVGAVAVAILIISIVFWYPFVYSLAGTIAQLTLATERIAEGNFDVRLHSGRTDE
                     LGRLAEAVNALAEKLNGFVTGQKRFLGDISHELRSPIARLQMALELLSSSSRLSEEEQ
                     NRIHDIREEVQEMTHLVSELLAFSKAGLKGSSPEMKPVNLHESVRTVVERL"
BASE COUNT          285 a          423 c          491 g          420 t
ORIGIN      
        1 tctggagatt ggtgccgatg attacttacc aaagaccttt tcgccccgcg aactattggc
       61 caggttgaga gcggttttgc gccgagtgag gcggaatgtt gagccgaatc tcaaggtgtc
      121 cggcgagggg gctgagtctt ctaaggacga gctgaattta ggggctttga tcattgatct
      181 ggcttctcgc tcggcgacac tttccgggca gagcctggct ttgactccgg tggagttcga
      241 cttgctggtg gtacttggtc agagccgggg acgggtgaaa agtcgtgagc aactattgaa
      301 tgaaattcgc gatcgcaact acgatatctt cgatcgctca atcgatgtgc acatttcggc
      361 cctgcggcga aaactggggg atccgcctca gaatcctcgc tttatacgca ctgtacgctc
      421 cgccggttat atgctcttaa atcaaagtga gagtgaggga ggcgatgaaa gctaggacct
      481 cactttattt gcaagttttc aatttgctgg tcttgtatct gctcttgctt tgcgccatcg
      541 tcttttttgt ctttaactca gccttcggct ttggctggga gactttaatc gccagtccca
      601 tcggcgagcg tgttgatacc gtggccggtg cggtaagcgg gcaactgcgc ggccggccga
      661 cctcacggtt caacgaggtc ttgcaggatt ttgggcgtat ctacaacgtt cagttttata
      721 tattcgacgg tgcggggcag ctagccggcg agaaagtgga cttgccccct gccgtagccg
      781 gacgtgtgca gatgctcctg ccgcctggtg ctatgggcga tcgcggaccg cggccgccta
      841 tggctttgcc tttacccggg ccctttcctt tgcgggtaca gggttttcct gagggcggcg
      901 cggccccgcc tcctcacggc tccgggcgtt tcatagtgca cagcggcagg gatggacgct
      961 tctggattgg caccaggttg cctctagtcg gtgatggggc cgggatgagt cctgttttgg
     1021 tgccggggcg gcaattctat cctccgggtt tccccgaacc ttttcatccg gccgtgctgc
     1081 ttgccgccag cgacaatatc tggcagagca gcctggtcat cgattttgcc ttcgtcggag
     1141 ccgttgccgt tgctatcttg attatctcca ttgttttctg gtaccccttt gtctatagcc
     1201 tggccggtac cattgcccag ttgactctgg ctactgagcg tatagccgaa gggaatttcg
     1261 atgtgcgtct gcactctggg cgtaccgatg aattggggcg gctggctgag gccgtcaatg
     1321 cgctggctga gaaattaaac ggttttgtca ccggacagaa gcgtttcctg ggtgatatat
     1381 ctcatgaact gcgctcgccg attgcccggt tgcagatggc tctggaactg ctttccagct
     1441 cctcccgcct gagcgaggaa gagcagaacc gcatccatga cattcgtgag gaagtgcagg
     1501 agatgaccca tctggtcagt gaactgctgg ccttttccaa ggccggtctc aagggcagtt
     1561 cgcctgaaat gaagccggtc aatctgcatg agtcggtgcg cacggtggtg gagcgcctg
//
LOCUS       DAOQNZ010001069         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58798_length_1618_cov_5.401152, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001069 DAOQNZ010000000
VERSION     DAOQNZ010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58798_length_1618_cov_5.401152"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKW73_16430"
     CDS             <1..702
                     /locus_tag="PKW73_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74939.1"
                     /translation="ASVPISQVFNTVKERVQEQVVRERGVLQTPIVETSKWSGKELVL
                     GCEPIRPRPGLPELPEETQKPQPAVVASATLPAPRNQPVAAPTKSFASDRPDNQPQSS
                     AQTRPVTTSASTSVSGGGDSPRRVAKSSAIDFLRYHFRIVGEKLYLDAWDDLGRNYRA
                     KFKNDPRAYQASVSRHRWLDYNASDSDFSILPGQGQTIKVRVRMNRLTGFNGYWVYTI
                     STFGGLYAIEDVTVG"
     gene            complement(797..1345)
                     /locus_tag="PKW73_16435"
     CDS             complement(797..1345)
                     /locus_tag="PKW73_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74940.1"
                     /translation="MPTAFVYPTNAGDANLVTSEQKSRIQSLLSKMFNTLSPAPLPDF
                     DAADSHIYSWFPQDSVLVSEESVLIVEPPLLDEARESDMPAPGVLYEHLTDSAAQKPE
                     IDGFSLPALKMERIPARRAQGGDIVAIKDRLDGKAHTALVVLEKDGQLYAHTKLSWGW
                     DKRNLEQLRRQNLVLAAYRLNQ"
BASE COUNT          347 a          414 c          460 g          397 t
ORIGIN      
        1 gccagtgtgc cgatttcaca ggtgttcaat acggtgaagg agcgtgtgca ggaacaagtt
       61 gtacgtgaac ggggtgtgct gcagacaccg attgtggaga ccagtaagtg gtcgggcaag
      121 gagttggttt tgggttgcga gcccatcaga ccaagacccg gcctgccgga gctgccggaa
      181 gagacacaaa agccccagcc ggcagttgtg gcttcggcta ctttgcctgc ccctcgcaac
      241 cagcccgtag ccgctccgac aaagagcttt gcctccgaca gaccggacaa tcagcctcag
      301 agttcggctc agaccagacc agtaactacc tctgcatcta catcagtttc agggggcggt
      361 gactcaccgc gccgggtggc aaaatccagt gcaattgatt ttctgcgtta ccactttcgc
      421 attgtggggg aaaagctcta cttagatgcc tgggacgatc tgggtagaaa ttaccgggct
      481 aagttcaaga acgatccccg tgcctatcag gccagtgtct ctcggcaccg ctggctcgat
      541 tacaatgctt ccgacagcga tttttccatc ttgcccggtc aggggcagac tataaaagtg
      601 cgggtgcgta tgaaccggct gacaggtttc aatggttatt gggtttatac catttccact
      661 ttcggcggac tttatgccat tgaggatgtg accgtgggct gaatcgttct ttctttgcct
      721 tctcagcctt ctctgccttc atcagttttg gcggtaaact tcgctgacgt ttgtctcaag
      781 ttctcttcag tcacccttac tggttgaggc ggtaagcggc cagaaccaga ttctggcgac
      841 gcagttgctc aaggttgcgc ttatcccagc cccaggagag tttagtgtgg gcatagagct
      901 gaccgtcttt ttccagcact acgagcgccg tgtgtgcttt gccgtccagt ctgtctttga
      961 tggctactat gtctccaccc tgagcccgtc tggccggaat tctctccatt ttcaaagccg
     1021 gcagggaaaa accgtcgatt tccggttttt gagcggcgct gtcggtgagg tgttcgtaga
     1081 gcacaccggg ggcaggcatg tcggactccc tggcttcatc gaggagaggc ggctcaacaa
     1141 tgagtaccga ctcttcagaa accagaacgg aatcctgcgg gaaccaggaa taaatatggg
     1201 aatcagccgc gtcgaagtcc ggcagcgggg cgggggacag ggtgttgaac atcttggaga
     1261 gcaggctctg gatgcggctc ttctgttcgc tggtgacaag attggcatcg ccggcgttgg
     1321 tcggatatac aaatgcggta ggcacggggc tgtcctcgct gggttataga aaagagagag
     1381 cgtcggggag ttttggcagg tgaggctaaa cggttcgatg aactcagatt acggcgacca
     1441 gcataaaaat ctcgtgaaat agattgcccc gggcaatttt aatttcgcca gcgtaagatc
     1501 cccactgacc acctcttcag ctttaactac tatgtgcttc tcccccgttt tcttgagaat
     1561 tagttcttat ggcaaaagac gaagcctgcc agagttttaa atcattcgat gcgctggc
//
LOCUS       DAOQNZ010001070         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58967_length_1616_cov_3.310698, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001070 DAOQNZ010000000
VERSION     DAOQNZ010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58967_length_1616_cov_3.310698"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKW73_16440"
     CDS             <1..475
                     /locus_tag="PKW73_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74941.1"
                     /translation="RQAVLEQLEDRYELNARDERKGLLDRSWKLSSYLRSLPRGSRKE
                     TLAEMKKDLASLARVARMVSWQPQYVELNPSQERLAETVLKLERDVYNIKRPRQLAKR
                     RAHVKIGSPIDLSPYMDEYERAPHEARHHIADKLRDHLQELIDAMASNIEKSRAL"
     gene            complement(470..880)
                     /locus_tag="PKW73_16445"
     CDS             complement(470..880)
                     /locus_tag="PKW73_16445"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNA74942.1"
                     /translation="MPYRAVVSSELARLFGVLSHPMRIRIIEELRKEDLPVNALRDIL
                     GITHAAVSQQLAVLRNCRLISEHREGRNVYYHLRNPKLAAWIIEGVNFISPDPSEVKE
                     MMSAIEHTRTVWHKEDQGVAEVKPAPQKKSRAVS"
     gene            complement(996..>1616)
                     /locus_tag="PKW73_16450"
     CDS             complement(996..>1616)
                     /locus_tag="PKW73_16450"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA74943.1"
                     /translation="PKAIPKDSLPALSPSPRRLTDSEWFDGRRKAIELRPELKKINLE
                     REQVKVELKLAQNMLLPAMNLYYMQGADTGPQGIGLVMRAGVNVSAPLRQRTARGQIE
                     AARLKIEKLNFDEKAEKQRIQAEVDDTVSAINTSYDRFAATEQEVKKAKDVEAGERLR
                     YGAGDSTLFLVNQRERTTAEAQMRLLETHVEYLQALSAFKAVTCRL"
BASE COUNT          407 a          436 c          412 g          361 t
ORIGIN      
        1 aaggcaggcg gtcttggagc aactggaaga tcgctacgaa ctaaacgcca gggacgagcg
       61 caaggggctc ctggatcgca gttggaaact cagttcctac ctgagaagcc tgcccagagg
      121 ctccagaaag gaaaccctgg ctgaaatgaa aaaggatctg gcctccctgg ctcgggtggc
      181 gcgcatggtc agttggcagc cccaatatgt ggaactcaat cccagccagg agagactggc
      241 tgaaaccgta ttgaaattgg aaagagatgt ctacaatatc aaacgcccga ggcaactggc
      301 gaaaagacgt gcccatgtga aaataggcag ccccatagat ttaagccctt acatggatga
      361 atacgagaga gccccccatg aagcccggca ccacattgcc gacaagctga gggaccacct
      421 gcaagagctg attgacgcca tggcaagcaa catagaaaaa agccgcgctc tatgaaaccg
      481 cccttgattt cttttgaggg gcaggcttaa cttccgctac accttgatct tctttgtgcc
      541 agactgtgcg agtgtgttcg atggcactca tcatttcctt cacttccgat gggtcgggag
      601 aaatgaagtt gacgccttcg attatccagg cggccagttt aggattgcgc aaatggtagt
      661 agacattgcg accttcccga tgctctgaga taaggcggca attgcgcaac acggccaact
      721 gctgggaaac ggcagcatgg gtgataccca agatgtcgcg taaggcattt acaggcagat
      781 cttctttgcg cagctcttca ataatgcgaa tgcgcatggg atgcgacaaa accccgaaca
      841 aacgggcaag ttcagaacta actacggctc tgtacggcaa gtgcgccccc tttgcttgat
      901 cgactcgatc aaacccatgc atatatcagc atatatgatt atattgaata aagtcaagca
      961 ccctgccatt aagaacctct ccaacaggca tcaaattata accggcaggt aactgcctta
     1021 aatgcagaca gcgcctgcag atactctaca tgggtctcaa gcaagcgcat ttgggcctcg
     1081 gccgtggtgc gctccctctg gtttacgaga aagagcgtac tgtctccggc gccgtagcga
     1141 agacgttccc cagcctccac atcctttgcc tttttcactt cctgctctgt ggcggcaaag
     1201 cgatcataag aagtattgat agcagatacg gtatcgtcaa cttcagcctg aatccgctgt
     1261 ttctctgcct tttcatcaaa attgagcttc tctatcttga ggcgggccgc ctcaatttgt
     1321 ccgcgcgcag ttcgctgcct gagcggtgct gagacattta cgcccgctct cataaccaga
     1381 ccgatgccct gggggccggt atcggcaccc tgcatgtagt aaaggttcat ggccggtaga
     1441 agcatgttct gagccaactt aagctcaacc ttgacttgct cgcgctccag attgatcttc
     1501 ttcagttccg gtctcagttc aattgcctta cgccgcccgt caaaccattc actatcggtc
     1561 aagcgcctgg gagaagggga aagagccggc agcgagtctt tgggaatagc cttcgg
//
LOCUS       DAOQNZ010001071         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_58968_length_1616_cov_3.269058, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001071 DAOQNZ010000000
VERSION     DAOQNZ010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58968_length_1616_cov_3.269058"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1325
                     /locus_tag="PKW73_16455"
     CDS             96..1325
                     /locus_tag="PKW73_16455"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA74944.1"
                     /translation="MLRLENRLRNLLSVLLCLILLPLLSGCVAVFDKLPHKKWGFIDK
                     SGRLVIPYKFDDVARDQHGGWTLPHKTFVNFSQGLCAVRVGNKWGYIDKNGLWKVPLK
                     YDSAGNFSDGLALVRVADRYGYIDKSGREVIPASFDIARIEGAKNDNPDWDFTQTLLT
                     PLEFSEGLAVACRDGKFGYIDKTGKFVIEPVYMRAEPFRQGLAAVNPFPVPAAVGGRT
                     FIDKSGKVVAPASQNCIDFSEGVFLAGNGRYDSGRRLFYLSEAGARITSEEFEDARIF
                     SEGLAAVAPKFDSANENKAYGYVDKSGKIVIKPGFDISGNNLAGNFRHGRAVVSRTVE
                     DALGNRSNLHGIVDTSGRFLVKPSYSHISSYCDGLARALIDNHTVYLDMNGKVAIETR
                     SVWGNSFSEGLAAVMEP"
     gene            1398..>1616
                     /locus_tag="PKW73_16460"
     CDS             1398..>1616
                     /locus_tag="PKW73_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74945.1"
                     /translation="MKSLKLYHGATLIGTITNYTVEDLFQWSGEIELTLEGVEYQHVF
                     DSLADDEAMMEGSDLPFDESYLEGWYIED"
BASE COUNT          370 a          363 c          478 g          405 t
ORIGIN      
        1 aagagcagca gtggctagca aagagtaagt gcaaccggca atacgtgtaa aatagcgtgt
       61 tgccggttgt gcttttttac tgggagactt gcattttgct acgcttggaa aatcggctca
      121 gaaacttgct cagtgtttta ctctgtctta ttctcttgcc gcttttgagc ggctgcgtgg
      181 cggtcttcga caagttgcct cacaagaaat ggggctttat cgacaagagc gggcgcctgg
      241 tcatacccta taagtttgat gatgtagcca gagaccagca cggcggctgg actttgcccc
      301 acaagacttt tgtgaatttc tcccagggac tttgtgccgt cagagtggga aacaaatggg
      361 gctatattga taaaaacggc ctctggaaag tgccgctcaa gtacgatagc gccggaaatt
      421 tcagcgacgg tctggctctg gtgcgggtgg ctgaccgcta cggctacatt gataagagcg
      481 ggcgggaagt tataccggca agctttgata ttgctcgcat tgaaggtgcc aaaaatgaca
      541 atcccgactg ggatttcacc cagaccctgc tcacgcccct ggagttcagt gaggggctgg
      601 ccgtagcctg ccgggatggt aagtttggct acatagataa gactggaaag tttgtcatag
      661 agcctgttta tatgcgggcc gagcctttcc ggcagggcct cgctgctgtt aatccctttc
      721 cggttccggc cgcggtggga ggacgcactt ttatagataa gagcggcaag gtggtggcgc
      781 ccgcctctca aaattgcata gacttcagtg agggtgtttt ccttgccggt aacggcaggt
      841 acgactccgg gcgcaggctt ttttacctgt ctgaggccgg cgccaggatt accagtgagg
      901 agtttgagga tgcccgcatt ttcagtgaag ggctggcagc ggtggcgccg aagtttgact
      961 cggcaaatga gaataaagcc tacggctatg tggataaaag cggcaaaata gttattaagc
     1021 cgggttttga tatctcgggc aacaatctgg ccgggaattt ccgtcatggt cgagctgtgg
     1081 ttagtcggac cgtcgaagat gctctgggta accgcagcaa tttgcatgga attgtcgaca
     1141 cttccggccg ctttctggta aaaccctcct acagtcatat ctcttcctat tgcgacggtc
     1201 tggcccgggc gcttatagac aaccatacgg tctaccttga tatgaacggc aaggtggcaa
     1261 ttgagaccag gtcggtctgg ggcaacagtt tcagcgaggg gttggctgct gtaatggaac
     1321 cctgagggct gccccttgaa agagtttggc gggaacggtt atatttgaga aacagcggtt
     1381 cgggggatgc cggagtaatg aaatcactca agctttatca cggtgcaacg ctgattggca
     1441 ccattactaa ttacacagtg gaagatctct ttcagtggag tggcgaaatt gagctgaccc
     1501 ttgagggagt cgagtaccaa catgtttttg attctctggc cgatgatgag gccatgatgg
     1561 aaggcagcga tttgcccttt gatgaaagct atctggaagg ttggtacatc gaagac
//
LOCUS       DAOQNZ010001072         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_59272_length_1612_cov_3.658960, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001072 DAOQNZ010000000
VERSION     DAOQNZ010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59272_length_1612_cov_3.658960"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKW73_16465"
     CDS             <1..285
                     /locus_tag="PKW73_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74946.1"
                     /translation="VDRWGDMTFFVCRMLPVVRTFISFPAGVLKAHFLMFNIFTFIGS
                     LIWCYFLTWVGVYFGENMETFRSIWHKFDLVIVIICVGGFGYYLYRHLKH"
     gene            complement(327..851)
                     /locus_tag="PKW73_16470"
     CDS             complement(327..851)
                     /locus_tag="PKW73_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74947.1"
                     /translation="MDKKHVAPDLKIGTDSHGGSDGNPLDRKINEETSKANMANSDFE
                     SAEEAAPEVDERDFPREIIPGFHQVKPETANSAVGASGAVYASGFTTRSGASSKYVSY
                     TIKLPSEQLAALQNIWLELKKLYGTHSPDKSGMIQLAIQDWLKRWDGPDKQKLLNELL
                     EIREDTRRRQYKKG"
     gene            1032..>1612
                     /locus_tag="PKW73_16475"
     CDS             1032..>1612
                     /locus_tag="PKW73_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74948.1"
                     /translation="MLVDIIRAILERVLRDKLILGLIIIGILGIFVTGANKDPGKDRL
                     VSNQEEEAAGSEQKPGQQKGQAAAGQGQRPGGQQQAGQGAPPPPAETPQQTANIACEF
                     IKWWLTKAMDYQQTTAQAAHKEALSWMKPPATEAFVQLFWSDELAAQVESGARTGSFQ
                     TSMVSPLAVNPDGSIPIRAIGVLVLQDTGYPPK"
BASE COUNT          381 a          422 c          414 g          395 t
ORIGIN      
        1 gtggatcgct ggggcgacat gacctttttc gtctgccgta tgttgccggt ggtgagaact
       61 ttcatatcct ttccagccgg tgtattgaaa gctcacttcc tcatgttcaa tatcttcacc
      121 ttcataggct ccctgatctg gtgttacttc ctcacctggg taggagtcta tttcggcgaa
      181 aacatggaaa ctttccgcag catctggcac aaattcgacc tggtgattgt gattatctgc
      241 gtaggcggct ttggctatta cctctaccgc cacctcaaac actaataacc ttctaaaaaa
      301 tacgctctac tcaaaacggg ttggcttcag ccctttttgt attgcctgcg gcgggtgtcc
      361 tcacgaattt ccagcagttc attgaggagc ttttgcttat cggggccgtc ccagcgcttg
      421 agccaatctt gaatagccag ctggatcatg ccgcttttat ctggagaatg agtcccgtat
      481 agctttttga gttccaacca gatgttttgc agagccgcca gttgttcgga aggcaatttg
      541 attgtatagc ttacgtactt ggaagaggca ccggagcgag tcgtaaagcc ggaggcatag
      601 acggcgccgg aggcaccgac cgccgaattg gcggtttccg gcttgacctg atgaaaaccg
      661 ggaataatct cacgggggaa atcccgttca tctacttccg gtgcggcttc ctccgccgac
      721 tcaaaatcgc tgtttgccat attcgctttg ctggtttcct cgtttatctt cctatctaac
      781 ggattgccgt cactgccgcc gtgggaatcg gttcctatct tcaggtccgg agcaacgtgc
      841 tttttatcca aggaaatgcc ctcactcgat aaagcaatac gacaacaaca ataacaacat
      901 tgtatgtgaa agcaaggaaa gacggtctac tgacaagcga taatagaaga actcatgggg
      961 aaatcatcag tttccctgtt gatttttgct ctcgcagggg cttttataga gatgggatat
     1021 agagactatt catgctggta gatataattc gagccatttt agagagggtt ttgagggaca
     1081 aactcattct gggtttgatc atcatcggca tattgggcat ctttgtcacc ggtgccaata
     1141 aagatccggg caaagacaga ttggtctcca atcaggaaga agaggcggcc ggttccgagc
     1201 agaagcctgg tcagcaaaag ggccaggcag cggcaggtca gggacagcgg cccggcgggc
     1261 aacaacaggc tggacagggc gcaccgcccc caccggctga aacaccgcaa caaaccgcaa
     1321 atattgcctg cgaattcatc aaatggtggc tcaccaaggc catggactac cagcagacaa
     1381 cagcccaagc cgcccacaaa gaagctttga gctggatgaa accaccggcc acggaggcct
     1441 ttgtgcagct tttctggagt gacgaactgg ccgcccaggt agagtcgggg gcgcgcaccg
     1501 gctcctttca gacttcgatg gtttcccctc tggcagtcaa tccggacggc tccattccca
     1561 ttcgagccat tggcgtgctg gtcctgcaag ataccggcta cccgcccaaa ag
//
LOCUS       DAOQNZ010001073         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_59288_length_1612_cov_3.254978, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001073 DAOQNZ010000000
VERSION     DAOQNZ010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59288_length_1612_cov_3.254978"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1612)
                     /locus_tag="PKW73_16480"
     CDS             complement(<1..>1612)
                     /locus_tag="PKW73_16480"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA74949.1"
                     /translation="TVNLSLIQGMDEHAFPLKTLGDALALQSHIMEQLEKAEVCENAA
                     IRASYLTFLVVGGGFSGVEVAGEINDLVKSSARYFHNFKASDIKVVIVHSGKAILPEV
                     GENLRLFARREMEKAGVEVKLEAVAAKATKEGLRLTDGSLMKAATVVCTIGTTSVPLI
                     AKLNLPKNRGRLVTQPDMSIEGMPGLWALGDCGAVVNSLDGNLCPPVAQFAERQGYQA
                     AKNIVATIQGHKTAPFRYKMQGQLCSIGGRSAVAEIMGLKLSGRFAWFLWRGIYLMKL
                     PSFTQKVKVGIEWACDLLFGRTLAHLKADRTRRVGRAYFGSGDIIFKQGDPATEFFVI
                     EKGEVEIIAELTDGGSETIAILGPGDFFGESSLLERALRTRTVRARGETVCMVLGRNV
                     FNQISAALIPLKDAVARAALRRTSMWHNVPEVRTILSKLTLTHFLEPLPMPPVSLETS
                     VETVIADMEKFTADACYVVDKEGALAGIITRSDLLSALEKAPLADPDSDLPVTEIMVM
                     HPVAMEADEQMTAAIATMRDHDLKQLPV"
BASE COUNT          310 a          448 c          467 g          387 t
ORIGIN      
        1 atcaccggta gttgttttag atcatgatca cgcatggtgg ctatggctgc cgtcatttgc
       61 tcatccgctt ccatagctac cgggtgcatg accatgatct cggtgaccgg caagtcgcta
      121 tcgggatcgg ccaggggcgc tttttccagg gctgaaagca ggtcggagcg ggtgatgatg
      181 ccggccaggg ccccttcctt gtctactaca tagcaagcat cggccgtaaa tttttccata
      241 tcggcaatca ccgtttccac cgatgtttcc aggctcaccg gcggcatggg caggggctcg
      301 agaaagtgtg tgagggtgag cttgctcaat atggtcctca cttccggcac gttatgccac
      361 atgctggtgc ggcgcagagc ggctctggcc acggcgtctt tcagcggaat caaggcagcg
      421 gagatctggt tgaagacgtt gcgccccagt accatgcaaa cggtttcgcc tctggctctg
      481 accgtgcggg tgcgcagggc tctttccaga agtgaggatt ctccgaagaa gtcgccgggt
      541 ccaagaatcg ctatggtttc gctgccaccg tcggtgagtt cggcaattat ttccacctct
      601 cctttttcga tgacgaaaaa ctcggtggcc gggtctcctt gtttgaagat gatgtcgcct
      661 gagccgaagt aggctcgccc cactcttctg gtgcgatcgg ccttaaggtg ggcgagagtg
      721 cggccaaaga gcagatcgca ggcccattcg atgcctactt ttactttctg ggtgaaggaa
      781 ggcagtttca taagatagat gccgcgccag agaaaccagg caaatcttcc ggagagtttc
      841 aaacccataa tctcggctac ggctgagcgt cctccaatag agcagagctg accctgcatc
      901 ttgtaacgga agggggcggt tttatgccct tggatggtag cgacaatgtt ctttgccgcc
      961 tggtagccct gtctttctgc aaattgtgcc accggcgggc agaggtttcc gtcgaggctg
     1021 tttacaacag cgccgcagtc gcccaaggcc catagcccgg gcatgccttc gatactcata
     1081 tcaggctggg ttaccagccg accacgattc ttcggcaggt tcagtttggc gatgagcggc
     1141 acactggtgg tgccgatggt gcaaaccacg gtggcggctt tcatcaagct gccgtcggtg
     1201 aggcgcagtc cctccttggt agccttggcg gccactgctt ccagttttac ttccacgccc
     1261 gccttttcca tttctcggcg ggcgaacagg cgcaggtttt ctcctacctc cggcagaatg
     1321 gccttgccgg agtgcacaat cactaccttt atgtcggagg ctttgaagtt gtgaaagtaa
     1381 cgggcgctgc tcttgaccag atcgtttatc tcccctgcta cttccacgcc gctgaagccg
     1441 ccgcccacca ccaggaaggt cagataggaa gcccgaatgg ctgcattttc gcaaacctcc
     1501 gccttttcca gttgctccat aatgtgtgat tgcagagcga gagcgtcgcc cagggtcttg
     1561 agtggaaagg catgctcgtc catgccctgg atgagtgaga ggttgacggt at
//
LOCUS       DAOQNZ010001074         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_59552_length_1608_cov_4.477141, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001074 DAOQNZ010000000
VERSION     DAOQNZ010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59552_length_1608_cov_4.477141"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PKW73_16485"
     CDS             <1..882
                     /locus_tag="PKW73_16485"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNA74950.1"
                     /translation="VTNGTAILGLGNIGPVAGVPVMEGKSVLYEQLVGVSGIPILIDS
                     KDVDVIVNTVKQIAPTFGAIHLEDIAAPECFEIERRLQEELKIPVLHDDQHATAVVVL
                     SALMTIAVRSGLNLHTCRVGIIGLGAAGSGIAQLLLSYGVKEVFGTDLREEAARRLES
                     LGGTAVNLAGIMSDCDVVVTTTGVPGLIKPEMVKAGQIILALSNPDPEILPEVAMASG
                     ALFAADGRTINNALSFPGLFRGALKAQAERFTDAMKLAAAHAICRQTKLDNIVPSILD
                     REVHQRVADAVADAAYL"
     gene            complement(893..>1608)
                     /locus_tag="PKW73_16490"
     CDS             complement(893..>1608)
                     /locus_tag="PKW73_16490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNA74951.1"
                     /translation="MLVSVGERKQPVSLGADNKGLKPVLEKLKNKQGLKIVFWGDSIS
                     AGGFATAPQYFFTNRFQAGLSKLFPDNKLTVVNQAVSGSNTGWRLANFDKEVIAEAPD
                     LLIVEFLNDYTLRPEVLAENYKTIIAKAKAANIPLIICVPHPPVPSYVNASTWHAVID
                     GPYPKLLRKLCQEQGVTICDVAKRWDSIGKEGLKPKLLLVDGLLHPNNYGHKIYAEEL
                     LALFQKAPEKAGAATSTSK"
BASE COUNT          335 a          408 c          458 g          407 t
ORIGIN      
        1 gtgaccaacg gtaccgccat tcttggtctg ggtaatatcg gtccggtggc cggagtgcct
       61 gtgatggaag gcaagtcggt gctctatgag caactggtgg gagtaagtgg tattcccatt
      121 ttgatcgact ccaaagatgt agatgtaata gtcaacaccg tcaagcaaat tgcccccaca
      181 tttggtgcta ttcacctgga agatatcgcc gcgccggagt gtttcgagat cgaaagacga
      241 ctgcaagagg agctgaagat cccggtcttg cacgatgacc agcatgctac tgccgtggta
      301 gtgctctcgg cacttatgac catagctgtt cgcagcggtt tgaatttgca tacatgccga
      361 gtgggtatca tcggtctggg agcggccggt tccggcatcg ctcaattgtt gttgtcttat
      421 ggcgtcaagg aagtgttcgg caccgatttg agagaggaag cggctcgccg tctggagagc
      481 ctgggaggca cagccgtaaa tctagccggt atcatgtccg attgcgatgt ggtagtcacc
      541 accactggtg tacctggcct gattaaaccg gagatggtga aagcggggca gattatctta
      601 gccctctcca accccgatcc cgaaatcttg ccggaagtag ccatggccag cggtgctctc
      661 tttgctgccg acggtcgtac catcaataat gcgctctcct ttcccgggct ctttcgcggt
      721 gctctgaaag cccaggcgga gcgcttcact gatgccatga aactggcagc cgctcatgcc
      781 atttgccgcc agaccaagct cgataatata gtgcccagca tactcgatag agaagtgcac
      841 cagcgcgtag ctgatgccgt agcggatgcc gcctacctct gaggtgagag ggttatttag
      901 aagtgctggt agcagcgccg gctttctcgg gtgctttctg aaagagtgcc agtaactctt
      961 ccgcatagat tttatgcccg taattgttgg gatgcaggag cccgtctact agcagcagct
     1021 ttggttttaa gccttctttg ccgatgctgt cccagcgctt ggccacatcg caaatggtta
     1081 cgccttgctc ctggcagagt ttgcgcaaga gcttcggata gggtccgtct attacggcat
     1141 gccaggttga ggcgttgaca tagcttggca ccggcggatg gggaacgcag atgatcaggg
     1201 gaatattagc tgctttcgct ttggcgatga tggtcttgta gttctccgcc agtacttccg
     1261 gtctgagagt gtagtcgttg agaaattcta caatgagcag gtcaggcgct tccgcgatta
     1321 cctctttgtc gaagttggcc agtctccagc ccgtattcga acctgatacc gcctgattga
     1381 ccacggtcag cttgttatcg gggaagagtt tgctgagacc ggcttgaaaa cgattggtga
     1441 aaaaatactg aggggccgtg gcgaagccac cagcgctgat actatcgccc cagaagacaa
     1501 tcttcagacc ttgtttgttc tttagtttct ccagcaccgg tttcaagcct ttattgtcgg
     1561 ctcccaggct gaccggttgt ttgcgttcgc caacgctgac cagcatgt
//
LOCUS       DAOQNZ010001075         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_59562_length_1608_cov_3.827431, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001075 DAOQNZ010000000
VERSION     DAOQNZ010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59562_length_1608_cov_3.827431"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKW73_16495"
     CDS             complement(<1..425)
                     /locus_tag="PKW73_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74952.1"
                     /translation="MTRRNDLLALEEEVLIELGTNKDRLEKNFLLFLLPFWAIYAPMA
                     AVHTIYVPFAPFLLTLISLSCLAFLCFQLNNKIGFGKETIQLPGVMPRVIKCSDIESV
                     YIETGKHDSLVFFLKDEAVQARQMSKRQSLSLKGLTVEDV"
     gene            complement(479..1333)
                     /locus_tag="PKW73_16500"
     CDS             complement(479..1333)
                     /locus_tag="PKW73_16500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNA74953.1"
                     /translation="MSSESEKSQVKKAEKKREAPPPPNVAGGIIDSHAHVVEEFFQEE
                     KGAIIDRARNLGVKRLVNPAVTLKESELAELKRLTETYDFIYAGCGLHPHEAKDWTEE
                     SADLIKERLKLPKYVAVGECGLDYYYNNSSREEQVLAFSEQIKLALLLNKPLIVHCRD
                     AWQEAFDLISLHGKGQVRGVFHCFTGGPEHLEAIKALDFYVSFSGIVTYKNAEGIQEA
                     ARLVSNDRFLVETDCPFLSPQKVRGIRNEPSFVWWTAEKLCQLRNTDMEEISQLASEN
                     ATRLFSLN"
BASE COUNT          358 a          392 c          398 g          460 t
ORIGIN      
        1 acgtcctcca cggtcagtcc cttgagagaa aggctctgtc tcttgctcat ttgccgtgcc
       61 tgtacggctt catctttgag gaagaaaacc aggctgtcgt gtttgccggt ttcaatatag
      121 accgattcga tatcgctgca tttgatgact cgcggcatca cccccggcaa ttgaatggtt
      181 tctttgccga agcctatctt gttgtttagt tggaaacaga gaaatgccag gcaggacagg
      241 gaaatgaggg tgagcaggaa gggcgcaaag ggcacgtaaa tagtatgaac ggccgccatg
      301 ggcgcataga tagcccagaa gggcaaaaga aagagcagga aatttttctc caggcggtct
      361 ttgttggtgc ccagttctat aaggacttct tcttccagtg ccaggagatc atttcgtcta
      421 gtcattgtat ttagtaaagt cttccagctg gctgtctttc attttgcgct ctgccaaatc
      481 aattgagtga aaagagcctg gtggcattct cggaggccaa ttggctaatc tcctccatgt
      541 cagtatttct caactggcat aatttttctg ccgtccacca gacaaaagag ggctcatttc
      601 ttattcctct aaccttttgg ggggaaagaa aggggcaatc ggtctccacc aggaagcggt
      661 cgttgcttac cagtctggcg gcttcttgaa tgccctcggc atttttatag gtgactatgc
      721 cggaaaagga gacatagaag tccagggcct tgatggcctc taagtgctcg ggaccgccgg
      781 tgaagcaatg gaagactccc ctgacctgac ccttgccgtg caggcttatc agatcaaagg
      841 cttcctgcca ggcatcccgg caatgaacaa tgaggggttt gttcaagagt agggcaagtt
      901 ttatctgctc gctgaaggcc agtacttgtt cttcccggga actgttgttg tagtaataat
      961 ccagaccgca ttcaccaacg gcgacatact tgggcagttt gagccgctct ttgatgagat
     1021 cggcgctctc ttccgtccaa tcttttgcct catgggggtg cagaccgcag ccggcataaa
     1081 tgaagtcgta ggtttccgtc agcctcttta gttccgccag ttcactctcc ttgagggtga
     1141 cggccggatt gaccaggcgc ttcaccccca ggtttctggc ccggtcgatg atggcgccct
     1201 tttcttcctg gaaaaattct tctaccacat gggcgtggct atctatgatg ccgcctgcta
     1261 cattgggagg aggaggagcc tctcttttct tctcggcttt cttgacctgg ctcttttccg
     1321 attcgctgga catgggacct cagtttcttg agaatatatg ggggaattgc gatttacttt
     1381 ttttcgtttt gcagtaaatt taaagcatcc aattactgac aatctttgac cgatttttcg
     1441 gtcttttcaa gattgcttcc agaacttact attcggcaac ctataaaatt gtttgaacgc
     1501 tcccttgcag aagacttgcc atgaaattca aaacccttac cattttaagt ttttccctct
     1561 gtctgatgct tccaagcccg gctgctttta gccagggtac attgaaag
//
LOCUS       DAOQNZ010001076         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_59654_length_1607_cov_3.162371, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001076 DAOQNZ010000000
VERSION     DAOQNZ010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59654_length_1607_cov_3.162371"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1607
                     /locus_tag="PKW73_16505"
     CDS             <1..>1607
                     /locus_tag="PKW73_16505"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNA74954.1"
                     /translation="IHRTIKRAKSAFSKPRVVFRGKDICPEGYYIAETRFHKNGKLVA
                     YGLTKNGSDWEEWRVKNLNTGKDLPHILLRVRTGEIAFHPDGKGLIYWKRCKQPTITA
                     RRGSKKTPASGAGQSQPPDLTPLGKEVEVVYQPLSRSGRPKTLLKPLRDDTAYLSVSY
                     VSKDIALVRELVPRTTKGITYLKVFKSKSKQNSIIPLFGGKPNEHNFVGTRDGKLYFI
                     SFEHADRGELIALELNEKKLEISKPKVIIPQGQDILQRLRFTKESIIAQYLTGKGEAN
                     QLRVFRTDGTACGEIPLPFKGFVVNLSTNRQNQDVLYSILHFNEPVASYFYNLRSKRL
                     RLLFPQTVKPTADIVKLIQVKASDGKEIPMWMVHRKSVRPDKNTPALMNVYGGFRIAW
                     TPFHSYELLSWTALGGIWLQPYLRGGNELGKSWHDDATLEKKQRTYDDVFDCADYVIK
                     NKITSARKLAIQGTSNGGLTVGAVTTQKPGLFGAAIAVNGLFDMLKFADHTVGAHWEA
                     EYGSVSKKKQFQVLRGYSPCHNIPQHG"
BASE COUNT          451 a          446 c          395 g          315 t
ORIGIN      
        1 ccatccatcg caccattaaa agagccaaga gtgccttctc caagcccaga gtagtatttc
       61 gaggcaaaga catttgcccc gaaggctact acattgccga gacccgcttt cacaaaaacg
      121 gcaaactggt agcctacgga ctgaccaaaa acggctctga ctgggaggaa tggcgtgtca
      181 aaaatctcaa caccggcaaa gatctgcctc atatattgct gagggtgcgc acaggagaga
      241 tagcttttca cccggacggc aaaggtctaa tttactggaa gcgctgcaag cagccgacaa
      301 taaccgctcg aaggggctca aaaaaaacgc ctgcatcagg cgccggtcag agccagccgc
      361 cggatctaac accgctgggc aaagaagtag aagtggtcta ccaacccctg tcccgctcag
      421 gcagacccaa aaccctgctc aagccgctgc gcgatgatac ggcatatctt tccgtcagct
      481 atgtttccaa agacattgct ctggttagag aactggtgcc gcgcaccacc aaaggtatca
      541 cctatctaaa agtcttcaag agcaaaagca agcagaatag catcattccc ctcttcggag
      601 gcaagcccaa cgaacacaac ttcgtgggca cacgcgacgg caaactctat tttatttcct
      661 tcgaacatgc cgaccggggc gagctgattg ccctggagct gaatgagaag aagcttgaga
      721 tatcgaaacc gaaagtgatt attccgcaag ggcaggatat cttacaacgg ctgcgcttca
      781 ctaaagagag catcatcgcc cagtatctca ccggtaaagg ggaagccaat caactgcgag
      841 tcttccggac cgacggcact gcctgcggtg aaataccgct cccctttaaa ggctttgtgg
      901 tcaacctttc caccaacaga caaaatcaag atgttcttta ctccatactg cattttaacg
      961 agccggtcgc ctcttacttc tacaacctca gaagcaagcg gctgaggctg ctattcccgc
     1021 aaacggtcaa gcccactgcg gacatcgtga agctcattca ggttaaagcc agtgacggca
     1081 aagaaatacc aatgtggatg gtgcatcgaa aaagtgtcag gccggataaa aacacgccgg
     1141 ccctgatgaa tgtctatggc ggctttcgta ttgcctggac gcccttccat tcttacgaac
     1201 ttctatcatg gacggctctg ggcggcatct ggctgcaacc ctatttgcgc ggcggcaacg
     1261 aactgggcaa gagctggcac gatgatgcca ccctggaaaa gaaacagcgc acctacgacg
     1321 atgtgttcga ctgcgccgac tatgtaataa agaacaaaat caccagtgcg cgcaaactgg
     1381 ctattcaagg caccagtaac ggcggactga cagtgggagc cgtgaccaca caaaagcccg
     1441 ggctgtttgg agcagctatt gccgtaaacg gtctctttga catgctcaaa ttcgcagacc
     1501 atacggtagg agcccactgg gaagctgaat acggctcagt gagcaagaag aagcaattcc
     1561 aggtgctgag agggtattcc ccctgtcaca atatcccaca gcacggc
//
LOCUS       DAOQNZ010001077         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_59852_length_1604_cov_5.090381, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001077 DAOQNZ010000000
VERSION     DAOQNZ010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59852_length_1604_cov_5.090381"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="coaE"
                     /locus_tag="PKW73_16510"
     CDS             complement(<1..710)
                     /gene="coaE"
                     /locus_tag="PKW73_16510"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNA74955.1"
                     /translation="MLPCPGIDLEEQHMTIKSQTGNPVYVIGVTGGIGSGKSAVGTML
                     SQMGVTVFDTDHIARDLRDKPGPVQDQILARFGADLLNSDGSINREKLGRIVFDDPQA
                     RKDLEDILHPAIAAAKQERVEASEGIVAVLVPLLYETSSQSQYDEVWTVVVQPDVQLA
                     RLRARDKRSDEELKKRIAAQLPQDAKAALSLVVIDNSGDLAATQVQVAQAIAETRERF
                     AAKVALPAAAPADGATASP"
     gene            complement(1203..1352)
                     /locus_tag="PKW73_16515"
     CDS             complement(1203..1352)
                     /locus_tag="PKW73_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74956.1"
                     /translation="MLDPEQLTFAQWFWICFALVLAVVFLCRCMDINMEAGPVTSIYW
                     LCPGP"
     gene            complement(1380..>1604)
                     /locus_tag="PKW73_16520"
     CDS             complement(1380..>1604)
                     /locus_tag="PKW73_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74957.1"
                     /translation="AGKGRPPIRSLEDLGKWLVDQIVDQSIMIDPANWLALWTKMERA
                     RAQGNMEEARIIQAQLKHYYPGVNVGPVLK"
BASE COUNT          395 a          442 c          443 g          324 t
ORIGIN      
        1 ggcgaagcag tagcgccgtc agcaggagcg gccgcgggaa gagccacctt ggcggcaaaa
       61 cgctcgcggg tttcggcaat ggcctgagcc acctgaacct gcgtggcggc gagatcgccg
      121 gaattgtcga tgacaaccag ggagagtgcc gctttggcgt cctggggaag ctgagcagcg
      181 atacgcttct tcagctcctc gtcggaacgc ttatcgcgag cacgcagacg tgcaagttgc
      241 acatccggct gcaccaccac ggtccacact tcgtcgtact ggctctgaga actggtctcg
      301 tagagcaggg gcacaagcac ggcgacgatg ccttcgctgg cttcgacgcg ctcttgcttg
      361 gcggcggcga tggcaggatg caggatgtcc tccagatcct tacgcgcctg cgggtcatca
      421 aacacgatac ggccgagctt ctcgcggttg atggacccat cagaattgag cagatcggcg
      481 ccaaacctgg cgagaatctg atcttgaacc ggtccgggct tgtcgcgcag atcgcgggcg
      541 atatggtcgg tgtcgaacac cgtcacaccc atctgcgaga gcatcgtacc cacggcagac
      601 ttgcctgagc cgataccgcc cgttacaccg atgacataca cggggttacc cgtctgagat
      661 ttgatagtca tgtgttgttc ctcgagatct attccggggc aagggagcaa agacgcgctc
      721 cgtcatgtgc tccggagagg ttagggtcaa tggtgacctg aggcttttcg caatctgaac
      781 aagaggtttc accactcaag caagcttgag cagcagcaaa gttcaggtgt cgtgttggaa
      841 caatcggacg ccaatccgag agctacacac agaaaagtct tatgagtacc ttagaggcct
      901 caggtcacca gttaaaaagt agatatggaa atacgctatg gacagtaact aaaggattta
      961 actgatttca aatcatgaga tcagatcttt acggccgcct cataaaaccc tgcaaggaca
     1021 gcctctctta ccgccccagc gcttgacaac attaacgaaa ggcgcttctt gaggctggaa
     1081 ttgccaatat tttgcaccat gtgtgattag atggcaccca tgatcaagct cgcggtgcta
     1141 gcgaggctag gctagcaacc aggcagttcg gacaaaccga taccttcgga aaatccgaac
     1201 tatcaggggc ccgggcaaag ccagtaaatc gatgtaaccg gacctgcttc catgttgatg
     1261 tccatacaac ggcagagaaa gaccacggcc agcaccaggg cgaaacaaat ccagaaccac
     1321 tgggcaaagg tcaactgttc cggatcaagc atggcatacc ctgtcagaaa agtgagaagc
     1381 tacttgagca ccggtccaac attcacaccc ggatagtagt gcttgagctg agcctgaatg
     1441 atgcgagctt cttccatatt accctgtgcc cgggcccgct ccatcttagt ccagagagcc
     1501 aaccagttag ccggatctat catgatggac tgatcgacta tctggtctac caaccatttg
     1561 cctaaatctt ccagagagcg aatgggcggg cggcctttgc cggc
//
LOCUS       DAOQNZ010001078         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_59862_length_1604_cov_3.715946, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001078 DAOQNZ010000000
VERSION     DAOQNZ010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59862_length_1604_cov_3.715946"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /gene="fmt"
                     /locus_tag="PKW73_16525"
     CDS             complement(<1..214)
                     /gene="fmt"
                     /locus_tag="PKW73_16525"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="modifies the free amino group of the aminoacyl
                     moiety of methionyl-tRNA(fMet) which is important in
                     translation initiation; inactivation of this gene in
                     Escherichia coli severely impairs growth; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNA74958.1"
                     /translation="MLKIIYLGTPDFAVPCLEALINESDFQVLAVVAQPDRPKGRGNK
                     LMPPPTKVLAQAHGIPVLQPEKLAKAP"
     gene            complement(225..428)
                     /locus_tag="PKW73_16530"
     CDS             complement(225..428)
                     /locus_tag="PKW73_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74959.1"
                     /translation="MTGPNRDKLVYLRQWVGAKVNVKSVGERDYNGILTNIVFDGTRL
                     SYIMLDDRVLLNFEQVVEISLAK"
     gene            540..1427
                     /locus_tag="PKW73_16535"
     CDS             540..1427
                     /locus_tag="PKW73_16535"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA74960.1"
                     /translation="MSKCVTAVGASKARGLHLSLPFLLLLPAFLLLALMVIVPITILF
                     VFSLGVRDDLGRVVLSFQPGNYLRFFCGPYLACLFRSIVLSFVTMVSTLVIGYLLSYW
                     LAFCAPGKWRNFLVLLVVLPLWTSSLLRIYAWITILHPTGLVATLAGTCFPSYALPPL
                     LYTPFAVWVGMVYNYLPYMVLPLYTSIERLEPRLLEASSDLGASPLATLFRVLLPLTG
                     GGIIAGSIMVFVPSLGDYVTPDLMGGAKTMFIGNLIQNQFLSVRDWVFGSAVSTILIA
                     LVSVSVWIYLRYAAKETIG"
     gene            complement(1424..>1604)
                     /locus_tag="PKW73_16540"
     CDS             complement(1424..>1604)
                     /locus_tag="PKW73_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74961.1"
                     /translation="TVPSDELIAASIREAGRAYSDATEREDFLVRAGRAVCQLFRSDY
                     ERMLSIVKKIKPSNI"
BASE COUNT          277 a          424 c          412 g          491 t
ORIGIN      
        1 caggtgcctt ggccagcttt tccggttgca ggacgggaat tccgtgcgcc tgggccagca
       61 ccttggtggg gggcggcatc agcttattgc cccggccctt gggcctgtcc ggttgtgcaa
      121 ctacggcaag aacctggaag tcactttcgt tgatgagcgc ttccaggcac ggcacggcga
      181 agtctggtgt gcccaggtat ataattttca gcatttactc ggttttactt ggcaagcgat
      241 atttctacta cttgctcaaa gtttaacagc actctatcgt cgagcatgat gtaagacagc
      301 cttgtgccgt caaaaacaat atttgtaagg atgccgttat agtctctttc gcctacgctc
      361 ttgacattga ctttggctcc aacccattgt ctcaagtaaa ctagtttgtc tcggttgggt
      421 ccagtcatga gataccatac tttcttttgg gaaaattttg acttttatct tgattatagt
      481 tgagaatggg caggctaagc cgacctccac aagtgtaaga aattcttgag gaaagcctga
      541 tgagtaaatg tgtgacagct gtcggtgctt caaaggctag ggggttgcac ctctcgcttc
      601 cctttctctt gcttttgccg gctttcctgc ttctggccct catggtaatc gtgccgatta
      661 ccatactttt tgttttcagc ctgggagtaa gagacgatct cgggcgggtg gtcttgagct
      721 tccagcccgg caattatctg cgattttttt gcggacctta tctggcctgc ctctttcgct
      781 ccatcgtgct cagctttgtc accatggttt ccaccctggt gattggttac ttgctttcct
      841 actggctggc cttctgcgct cccgggaagt ggcgtaattt tctagtgctg ctggtggttt
      901 tgcccctgtg gacttcgtct cttttgcgca tctatgcctg gattaccatt cttcatccca
      961 ccggactggt ggccaccctg gccggcacct gctttccttc ctatgccctg ccgcccctgc
     1021 tctatacgcc ctttgccgtc tgggtgggca tggtctacaa ctatctgccc tatatggttt
     1081 tgccccttta cacttccata gagagattgg agccgcgctt gctggaagcc tcgtccgatc
     1141 tgggtgcttc gccccttgct acattgtttc gtgtgctttt gcctttgacc ggcggtggca
     1201 tcattgccgg ttccatcatg gtttttgtgc cttctctggg cgattatgtc acacccgatc
     1261 tgatgggcgg agccaaaacc atgttcattg gcaatttgat tcagaaccag ttcttgagcg
     1321 tgcgcgactg ggttttcggc tcggcggttt ccaccatttt aattgccctg gtatcggttt
     1381 cggtgtggat ttacctgcgt tatgccgcca aagaaaccat cggctagatg tttgagggtt
     1441 tgattttctt gacgatagaa agcatgcgtt cgtagtcgga gcggaagagc tggcacaccg
     1501 cccgtccagc ccgcacgagg aagtcctctc tctccgttgc atcactgtat gcccgaccgg
     1561 cttctctgat ggaggctgca atcagttcgt cggaaggcac ggtt
//
LOCUS       DAOQNZ010001079         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_59865_length_1604_cov_3.571982, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001079 DAOQNZ010000000
VERSION     DAOQNZ010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59865_length_1604_cov_3.571982"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..960
                     /locus_tag="PKW73_16545"
     CDS             106..960
                     /locus_tag="PKW73_16545"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNA74962.1"
                     /translation="MRELTERQAKYQGQTINVAVDERTLFAVLADRIAAMARAAVEKD
                     GLFTMLLSGGSTPRGLYQRLATLSEDIMPWGKTYLFLGDERCVSHADSDSNYKMISDN
                     LLSRISIPSTNVFPTIRQDIDPEDSARIYEQTIRRFFKRSAEGTAGVENGTFEGEANW
                     LKPSSESACESKNMPIFSLAIMGLGPDGHTASLFPDTAALAEEERLCVANFVPKFDTY
                     RLTLTMPVFANCRQLFFLVQGDGKTAIVQSVLKEPEKNYPAQQLIARCPQGAVEWYMD
                     EACTSSLI"
     gene            970..>1604
                     /locus_tag="PKW73_16550"
     CDS             970..>1604
                     /locus_tag="PKW73_16550"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNA74963.1"
                     /translation="MVDKKSDIGLVGLGVMGENLALNIERHGYQISIFNRSHEKVVEF
                     MEGRGKGKKVVGAPDEKSFVESLSSPRKIILLVKAGDAVDQTIAKLKPYLEKGDIIID
                     GGNSHYVDTQRREKELAAEGLCLIGSGVSGGEEGALWGPSLMPGGDAKAYEAIRPIWE
                     DVSAKVDGSPCVTYLGPDGAGHFVKMVHNGIEYGDMQLIAEVYDVLRRALGL"
BASE COUNT          370 a          383 c          465 g          386 t
ORIGIN      
        1 gtgcggcatg tccgaacttg ttgacgatgc tcttgagcac acccagcaag acgtttacta
       61 tctggaagcc gtgagcggac tattggaaat gtttcaaggc aggggatgag ggaattgacc
      121 gagcggcagg ctaagtatca aggacagact ataaacgtcg ctgtcgacga acggaccttg
      181 ttcgcggttc ttgccgatcg tatcgcggcc atggccagag cggctgtgga gaaggacggt
      241 ctctttacca tgcttttgtc aggcggcagc acccctcgcg ggctctacca gagactggct
      301 actctttcgg aagatatcat gccctggggc aagacctatc ttttcctggg tgatgagcgt
      361 tgtgtcagcc atgccgacag cgacagcaac tacaagatga tctccgacaa tctgctcagt
      421 cgcatttcca ttcccagtac caacgtcttt cctaccatca ggcaagatat agatccggaa
      481 gattccgctc gcatttacga gcagacgatt cgacgctttt ttaaacgcag tgccgagggc
      541 actgccggtg tcgagaacgg tactttcgaa ggggaggcca attggttgaa gccttcttcc
      601 gagagcgcct gcgagtctaa gaacatgccg attttttccc tggctatcat gggcttgggt
      661 ccggatggcc ataccgcctc actctttccg gacactgccg ctctggcgga agaggagagg
      721 ctctgtgtgg caaactttgt tcctaagttt gatacctaca gacttaccct cactatgcct
      781 gtttttgcta actgccgtca gctcttcttt ctggtgcagg gtgacggcaa gacggccatt
      841 gtgcagtcgg ttttgaagga gccggagaag aactatccgg ctcagcagtt aatagctagg
      901 tgcccgcaag gggcggtgga atggtatatg gacgaagctt gcacgtccag tcttatatag
      961 gagcaggaaa tggtagataa gaagagcgat attggccttg ttgggcttgg cgtcatgggt
     1021 gagaacctgg ctctcaacat agagagacat ggctatcaaa tttccatttt caaccgttcc
     1081 catgaaaagg tcgtggaatt catggaaggg cgcggtaaag gcaagaaagt tgtcggtgcg
     1141 cctgatgaga agagctttgt ggagtctttg agcagtcccc gcaaaattat ccttctggtc
     1201 aaggccggag acgcggtaga tcaaaccata gccaagctca aaccttacct ggagaaaggc
     1261 gacatcataa ttgacggcgg taattctcat tatgtcgaca cccagagacg tgagaaagag
     1321 ctggctgctg aaggactctg tctgattggt tccggggtat caggcggcga agagggcgct
     1381 ctctgggggc cgagcctcat gcccggcggc gatgcgaaag cttatgaagc aattagacca
     1441 atttgggagg atgtctctgc caaggtcgat ggcagcccat gtgtgactta tcttggtccc
     1501 gatggtgccg gtcacttcgt taagatggtg cacaatggta tcgaatatgg tgatatgcaa
     1561 ctgattgccg aggtctatga tgttctgcgg cgagccctcg ggct
//
LOCUS       DAOQNZ010001080         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_59944_length_1603_cov_3.841085, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001080 DAOQNZ010000000
VERSION     DAOQNZ010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59944_length_1603_cov_3.841085"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PKW73_16555"
     CDS             <1..837
                     /locus_tag="PKW73_16555"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HNA74964.1"
                     /translation="AEAVAAERELSSFTGKEVLERILARARQGNWQRLPMSELMGKVA
                     CEFLGTPYKAFTLELSSSAEIVSANLKYLDCVTFFETTLALSRLIKLGGSSAADLLKQ
                     IALLRYYGGAAGDFSTRLHYTSGWFIDNQRKGVVRLLDNLPGAMPFLLYVNFMSSHPS
                     AYPQLADAQLVEKIKAREEALNRERLSYVPLAKLAGVEPFLQSGDIVGVCTRLSGLDI
                     EHTGLVLRQGRGASSSVHFMDASSRKRNMKVVIEPGPLSRAFNWSSQLTGVVFARPLE
                     PK"
     gene            complement(838..1242)
                     /locus_tag="PKW73_16560"
     CDS             complement(838..1242)
                     /locus_tag="PKW73_16560"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNA74965.1"
                     /translation="MVMVKMVEDGAAPPEVQAVFDEIKELRKSNWINNFWKVLASSPA
                     TMLRIWSNVKQVMAPGALDPLTKEMIYVAVSVTNNCEYCIHSHTAAARAKGMTDEMYG
                     ELLAVVGLANETNRLANGYQMPVDEQFKPSFD"
     gene            complement(1261..1581)
                     /gene="trxA"
                     /locus_tag="PKW73_16565"
     CDS             complement(1261..1581)
                     /gene="trxA"
                     /locus_tag="PKW73_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNA74966.1"
                     /translation="MVKHSNSQSFKADVLETKEPVLVDFYASWCGPCRAMAPILEELA
                     GEYSGKVTVCKVDVDENQELAAKYEIRGIPALKVFKNGKIVNEVTGMISKSELAAILD
                     SANN"
BASE COUNT          307 a          429 c          436 g          431 t
ORIGIN      
        1 gcagaggctg tggcggctga gagggaactt tccagcttta ccggcaaaga ggttctggag
       61 cgcatccttg ccagggctcg gcaaggtaat tggcagcgtt tgcccatgtc cgaactgatg
      121 ggtaaggtgg cctgcgaatt tctcggtaca ccttataaag cctttactct cgagctttcc
      181 agctctgctg agatcgtcag cgctaatttg aaatatcttg attgtgtgac cttcttcgag
      241 accaccctgg ccttgagccg cctgatcaaa ttaggcggta gcagcgccgc cgatctgctc
      301 aagcaaattg ccttgctgcg ctattatggt ggcgccgccg gtgacttcag cacgcgcctg
      361 cattatacga gcggctggtt tatcgataac cagcgcaaag gggtggtgcg attgctggat
      421 aacttaccgg gtgccatgcc gtttttacta tatgtgaatt tcatgagcag ccatccttcc
      481 gcttatcccc agcttgccga tgcgcaactg gtggagaaga tcaaggcgcg ggaagaggct
      541 ctcaatcggg agcgacttag ctatgtgcct ctggctaagc ttgccggtgt ggagcctttt
      601 ttgcagagcg gcgatatcgt cggtgtctgt acgcggcttt cggggctgga tatagaacat
      661 accggtctgg tgctgcgtca gggccggggc gcttcctcgt cggtgcattt tatggatgct
      721 tcttcgcgca agcgcaatat gaaagtggtg atcgaacccg gaccattgag tcgggctttc
      781 aactggtctt cgcaattgac cggcgtggtc tttgcccgcc cgctggagcc caaatagtca
      841 gtcgaaactt ggcttgaact gctcgtctac aggcatctga tagccgttgg ccaggcgatt
      901 ggtttcgttg gccagcccca ctacggcaag caattcgccg tacatttcat cggtcatgcc
      961 tttagccctt gccgcagccg tatgggaatg gatgcagtac tcacagttgt tggtgacact
     1021 gaccgccaca tagatcattt ctttggtgag cggatcaagt gctcccggcg ccatcacttg
     1081 cttgacattg ctccagatgc gcagcatagt ggcgggagaa ctggccagga ctttccagaa
     1141 gttgttgatc cagttgcttt tgcgcagttc tttgatctcg tcaaaaacgg cttgcacctc
     1201 cggtggtgcg gctccgtctt ccaccatttt taccatcacc atagctattc tcctgttcga
     1261 ctagttattg gcggagtcta gaatggcagc cagttctgat ttggagatca tacctgtaac
     1321 ttcgttgact atcttgccgt ttttaaagac cttcagggcc gggatgcccc gtatctcata
     1381 tttggccgcc aactcttgat tctcgtctac atctaccttg cagacagtca ctttgccgct
     1441 gtattctccg gccaactctt ccaagatggg cgccatggcc cggcagggtc cgcaccagga
     1501 cgcatagaaa tcaaccagca ccggctcttt cgtttccaac acgtcggctt tgaagctctg
     1561 cgagttggag tgtttgacga tgcctttggc tgtgccttcc ttg
//
LOCUS       DAOQNZ010001081         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_60102_length_1601_cov_3.236093, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001081 DAOQNZ010000000
VERSION     DAOQNZ010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60102_length_1601_cov_3.236093"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="PKW73_16570"
     CDS             complement(<1..1455)
                     /locus_tag="PKW73_16570"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA74967.1"
                     /translation="MHAEDETLKAPALQNTGQEPLNSITFSMYHPTLRALHYGLFGLL
                     ALVYLWLTVSISLSNRHILTNPSACLGIFLPGLVAGALIYLAVRQRLCTISYDSQNLE
                     FAPIWNLQLHRRRKRPWQDIHSVQIVYPEAAATCTFPNFAGEKINWRQFLQTGGIGPC
                     LVFDFKSGGSATLHLSLLSRAQAEELFAAIDKFGDQSKLSSDFVKLQKAIVLEHVNAE
                     SFTQLWSEDLNPSYCATSYVPLPGRHLLQEGRYEILMELAAGGMSAVYLARHKENSNA
                     KVVLKESVLPQGVSEEQRKKARELFEREAHLLLKLKHPHIARVLDRFVEEGRDYLVLE
                     YIPGLTLRQLVQTRGKQKEKNVLNYCSKLLEILAYLHEQNPPLIHRDLTPDNIILRKD
                     GSLCVVDFGAANELVGQATGTMIGKQCYIAPEQLRGKASQASDLYALGGTLHFLLTGS
                     DPEALSASNPKDLVPEISSATSALINQLTAFEAEA"
BASE COUNT          332 a          402 c          464 g          403 t
ORIGIN      
        1 ggcctctgcc tcaaaggccg taagctggtt gattaaagca ctggtggcgc tggagatctc
       61 cggcacgagg tccttcggat tggaggcact gagagcttcc gggtcgctgc ctgtgagcag
      121 gaagtgcagg gtgcctccca gggcataaag atcgcttgcc tggctggcct tgccccgcaa
      181 ctgctccggt gcaatgtagc attgtttgcc gatcatggtg ccggtggcct gaccgacgag
      241 ttcgttggcg gcaccaaaat ccaccacgca gagactgccg tcctttctga gaataatatt
      301 gtcgggagta aggtcgcggt gaataagtgg cgggttttgc tcgtgcaagt aggcgagaat
      361 ctccagaagc ttgctgcagt aattgagcac gtttttttct ttttgcttgc ccctggtctg
      421 taccagttga cgcaaggtca aacccggtat gtactccaaa actagatagt cccttccctc
      481 ttccacaaaa cggtccagaa ccctggcgat atgcggatgt ttaagtttga gaagcaggtg
      541 cgcctctctt tcaaacagct ctcttgcctt ttttctttgc tcctcgctta ctccctgtgg
      601 gagaaccgat tctttcagaa cgactttggc attgctgttc tctttgtgcc ttgccaaata
      661 gacggcagac atccctccgg cagccagctc catcagaatt tcatagcgcc cttcttgcaa
      721 gaggtggcga cctggcagtg ggacatagct ggtagcgcag taactgggat tgagatcctc
      781 actccagagt tgcgtgaagc tttcagcatt tacatgttca agcacgatgg ccttttgcaa
      841 cttgacgaaa tcgctggaga gtttgctctg gtcgccaaat ttatctatgg cggcgaataa
      901 ctcttctgct tgcgctcggc tcaggagcga caggtgaaga gtggcggagc cgccggactt
      961 gaaatcgaaa acgagacagg gtccgatgcc gccggtttga agaaattggc gccagtttat
     1021 cttttctccg gcgaagttag ggaaggtgca ggtggctgct gcttccggat aaactatctg
     1081 gactgagtgg atgtcttgcc aggggcgctt ccgtcggcgg tgtagttgca ggttccagat
     1141 gggcgcaaat tccaggttct ggctgtcgta ggaaattgtg cagagacgtt ggcgtacagc
     1201 caggtaaatg agggcgccgg ccaccagacc gggcaggaaa atgcccaggc aggcactcgg
     1261 attggttaag atgtgcctgt tgctcaggct gatagacacc gtcagccata gatagaccag
     1321 ggccagaaga ccgaaaagac cgtaatgcag tgccctcaat gtaggatgat acatgctgaa
     1381 ggtaatgctg ttcaatggtt cttgaccggt attttgcaga gccggtgctt tcagggtctc
     1441 gtcttcagcg tgcattttgt cctattcctc ttcctccttg tgttccctgc gccctgcctc
     1501 agtaaaccgg accgggcggt gtcggaacct catgggaggt tattcacgta gagttcgatg
     1561 agcctttcgt cgggctgagg acatctgagc ggagccaggg c
//
LOCUS       DAOQNZ010001082         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_60457_length_1597_cov_3.339818, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001082 DAOQNZ010000000
VERSION     DAOQNZ010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60457_length_1597_cov_3.339818"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PKW73_16575"
     CDS             complement(<1..631)
                     /locus_tag="PKW73_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74968.1"
                     /translation="MTGLTCCQCTWAAEPLQGGIKDEAVLHPSLTVIPAGVKSQAPLR
                     GSLDSNYLQASTSVIQLSGNHFTSGQPTYALPPTGQYTPQLNAQIPYSSYGPIRTYGE
                     HGIIAPISTYKQDLRTNITSVSPQMSSTPSYSAKGITTWGTPQPAATPALSTHEGITT
                     YVPGYEVKTVSRPSSLVTAYHCMHGNLPFLCPGHCTILPGYSATHQDYKV"
     gene            complement(824..>1597)
                     /locus_tag="PKW73_16580"
     CDS             complement(824..>1597)
                     /locus_tag="PKW73_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74969.1"
                     /translation="PPAYQGRALVLAPLLHPGLPGISAGQTNRPVKVARLEQKLIAYG
                     TSELLIGENGIEYRIPKQNLRILAFAPDWKLMVYNTKTKMASVQPYEQWSRKITGKEA
                     LLVKSRQTRAAYYQGKPARLTILSLLPSESDVSKSEFIYRDADGRAQQYNRIELLEPL
                     ATNIKLKPQQAEFIRWKLGVPQAGGLVVQQQNFYPSGKRDQMLETISISQTTVSPDAW
                     RYPSDFTRGSMATVKEEKKKYMQAAEMFGTLILDKKEDK"
BASE COUNT          324 a          371 c          475 g          427 t
ORIGIN      
        1 ctactttgta gtcctggtga gtggcggaat agccgggcaa aatcgtacag tggcccggac
       61 agagaaaggg caggttgccg tgcatgcaat ggtaagcagt gacaagactg ctaggtctgc
      121 ttactgtttt tacttcgtag cccggcacat aggttgtgat tccttcatgg gttgaaaggg
      181 ccggtgtggc ggccggctgc ggtgttcccc aggtggtgat gcctttggcg gagtagctgg
      241 gggtgctgga catctgaggc gagacgctgg tgatattagt acgcagatct tgcttatagg
      301 tcgagattgg cgcaatgatg ccgtgctccc cataggtgcg aatgggacca taagaggaat
      361 atggaatctg tgcgttcagt tgaggtgtgt attggccggt gggcggcagt gcataagtag
      421 gctgcccgga ggtgaagtgg tttcccgaca attggatcac cgaagtagaa gcttgcaggt
      481 aattgctgtc gagagagccg cgcaaaggtg cctgactctt tacgcctgca ggaatgacgg
      541 tcagggatgg atgcaagacc gcttcatctt tgatgccgcc ttgcaaaggc tctgccgccc
      601 aggtgcactg gcagcacgtc aagcctgtca tcaggagtac gcttagccgt aaatttttgg
      661 agagtatttt gagttgcttc attgtgttga ccgctgatgc attcaacata gcatcgcgtg
      721 aacttaaaac ggttgcggtc agactcctga atggctcatc cagtgaactt cccagtgctt
      781 tattccccgc ttacggtcat aatatttagt ttgcaatctg ccactatttg tcttctttct
      841 tgtccagaat gagggtgccg aacatctcag ccgcctgcat gtatttcttc ttctcttcct
      901 ttacagtagc catacttcct ctggtgaagt cgctgggata gcgccaggcg tccggagaaa
      961 ctgtagtctg gctgatactt atggtctcca acatctgatc tcttttgccg gaagggtaga
     1021 agttttgctg ctgcaccacc agtccgccgg cctggggaac acccagcttc cagcgaatga
     1081 attccgcctg ctggggtttc agtttaatgt ttgtcgccag aggctccaga agttcgatgc
     1141 gattgtattg ctgggctctg ccgtcggcgt cccggtaaat gaactcactt ttggatacat
     1201 ccgattcgga cggtaaaaga gaaaggatgg tcaggcgcgc cggcttgccc tggtagtagg
     1261 cggctctggt ctgtctggat tttacaagga gggcttcttt gccggtaatt ttccggctcc
     1321 attgctcgta tggttgaaca ctggccatct tggttttggt gttgtagacc atgagcttcc
     1381 aatccggggc aaaagccagg atgcgcaggt tttgtttggg aattctatat tcaataccgt
     1441 tctcgccgat aagcagctct gaggtgccgt aggcaattag tttttgctcc agtctggcaa
     1501 ctttaaccgg ccggtttgtc tggccggcgc tgattccggg caagcctggg tggagaagag
     1561 gagcaagaac gagagcccgg ccctggtaag caggcgg
//
LOCUS       DAOQNZ010001083         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_60508_length_1596_cov_4.637249, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001083 DAOQNZ010000000
VERSION     DAOQNZ010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60508_length_1596_cov_4.637249"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKW73_16585"
     CDS             <1..457
                     /locus_tag="PKW73_16585"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNA74970.1"
                     /translation="ELTGLAAAGLAGGAGAATAGEAPAWLPHFATPTLADAMDAISSA
                     RLVVAVDTGLMHMALHQGIATVALFRSNTMFLRPGPHCRNLVAPQCDPACLKQEFSAV
                     PNKMLNYNKGNQSYTYWKTWDCALSNPAERCMSRISVGEVYEAVRDLLS"
     gene            complement(480..1166)
                     /locus_tag="PKW73_16590"
     CDS             complement(480..1166)
                     /locus_tag="PKW73_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74971.1"
                     /translation="MYRGFSSIESDMRDRLVEMTSTHEIPARWRGTPIEHYIQAQNLG
                     YPLHPSETPQILISTCIEFRYALPIPAKFAYVIRTAGGRLVGQEFAVAYALTRGVRNI
                     LLIAHDDCGMAKVRNNRPELTKALTDQGWSQEAAAKFVAHHCENDAIVDELESLETEY
                     HRLKRIFRDVHVAPLFLTLQDKRVHLPKWYHTLINSAEPQAKPVLQPGCVSDEDLRAC
                     VSSYFRGTAR"
BASE COUNT          316 a          412 c          450 g          418 t
ORIGIN      
        1 cgagttgacc ggcttagccg ctgccggact ggctggcggt gccggggcgg ccactgcggg
       61 cgaggctcct gcctggctac cgcactttgc cactcccacc ctggccgatg ccatggatgc
      121 cataagcagc gctcgcctgg tggtagccgt ggataccggc ttgatgcaca tggcgctgca
      181 ccagggtatt gccacggtgg ccttatttcg cagcaatacc atgttcttgc gtcccggtcc
      241 gcattgccga aatctggtgg caccccagtg cgaccccgct tgcctcaagc aggaattctc
      301 ggccgtgcct aataagatgc ttaattacaa caagggcaat cagtcataca cttactggaa
      361 gacctgggat tgcgccctgt ccaaccctgc tgagcgttgc atgagtcgca tcagtgtcgg
      421 cgaagtgtat gaagcagtgc gcgatctctt gtcttgatgc gagatttagc ccgggtaaat
      481 taacgtgccg tgccccggaa ataagatgag acacaggcgc gtagatcttc atcgctgacg
      541 cagccaggtt gcagcaccgg tttcgcttgc ggttcggcac tgttgatcaa ggtgtgatac
      601 cacttgggca agtgcacgcg tttgtcttgc agggtgagga aaagcggcgc cacatgaaca
      661 tcgcgaaaga tgcgcttgag tctgtggtat tcagtctcca gcgattcaag ctcgtctacg
      721 atggcgtcgt tttcacagtg gtgagcgacg aatttggcgg cggcttcctg gctccagccc
      781 tggtcggtga gagctttggt caactccggt cggttattgc gtaccttggc catgccgcaa
      841 tcatcgtggg cgatgagcaa gatattgcgc acgccccggg tgagagcata ggctacggca
      901 aattcttgcc ctaccaatcg gccgccggcg gtgcggatca cataggcaaa tttggcggga
      961 atgggcagtg catagcgaaa ctcaatgcag gtggaaatga gaatctgcgg ggtttccgac
     1021 ggatgaagcg ggtagccgag attttgggcc tggatgtaat gctcgatggg agtgccgcgc
     1081 caccgtgccg ggatctcatg ggtggaggtc atctccacca gccgatccct catgtctgat
     1141 tctatgctgg aaaaacctct gtacatcttt ctgctccgtt tgcgctgctt gtgtgctaga
     1201 cctgaaacgg tagaatggaa tgattttcaa attctcaaaa aacgtcttgt gcgccggctg
     1261 gaaatcgcgc ttttgatatt ggctattcag tcttgaattc tacgatttac ttggaaatca
     1321 gattgctgtt ctaccaacgg ctcttctgtc ttgcgtctgc tcgtgagctt ctgatttcct
     1381 tcttctgact ctgccactca gttcaaattg gttttgactg cgtgcttttg tcgtgcttgc
     1441 ttctaactct gtatttcgat ggtagcagtc tctgttatgc ccggaacttt ccataattat
     1501 ttcttcgtct gataaagccg ctatgtttag gggtgcctca ctccggcgta caaggttggg
     1561 tgttcgccat cttaaagata ctaaggcata tactag
//
LOCUS       DAOQNZ010001084         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_60922_length_1591_cov_3.386719, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001084 DAOQNZ010000000
VERSION     DAOQNZ010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60922_length_1591_cov_3.386719"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1591)
                     /locus_tag="PKW73_16595"
     CDS             complement(<1..>1591)
                     /locus_tag="PKW73_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74972.1"
                     /translation="KFEALGDSRGEARQNIVAINNKIAMNNLELFKEFAGRSSERARQ
                     EFQNEGGDKSLEKALVPEYKRQADGKYKFENGIFSPLSEPGRLMTIARDYVREGKTSA
                     LTKIEENNWGTGPSQEQLEKIFQNMSAADRGRFRLGFELASDLKKDQSDPRHIAASNL
                     LSETNQTNEAKQARDAHNYFVEFAAKSRDLHWFSKDIKEAKYLQGAYGSQAGLELDKH
                     AGFFTHGSAQNFMKSIENLDAATIKSLVENPELHAMLKAQAKDRYGEDARSTNALKLL
                     DQKLAMAKDFKEGNEEQLITLAPKLEKAENLEQFKAGRELSLHLESLNPVERLNQLQK
                     LRATEEGRKQLQNLDSFHNTIFEATRDNVRNDIKDSIKNANGISDIMLAIEKMTPAER
                     TNYSQSKEFRDSVAAVLKETLPYSQAGQEAALSLLRQIGENPAESPRKDALTRLLEEA
                     ATRGPSVAAGMKIINESLSTESGEKLRERLKVDPQLRESFERAVNAVFQVAIPEGEGS
                     TRYEILPDLESRIIMPAINGGL"
BASE COUNT          263 a          423 c          401 g          504 t
ORIGIN      
        1 ccagtccgcc attgatggcg ggcattataa tgcgactctc cagatccggt agtatttcgt
       61 agcgggtgct gccctctcct tccggaatgg ccacctgaaa gacagcattt acggctcgtt
      121 caaaagactc ccgcaactgc gggtctactt tcaacctctc tctcagcttc tcaccacttt
      181 cagtgctgag gctttcgttg atgatcttca tgccggcagc tacggaagga cctcttgtgg
      241 ctgcttcttc cagtaaacga gtaagggcat ctttgcgggg gctttctgcc gggttctcac
      301 ctatttgcct gagaagactg agtgcggcct cctgtccagc ctgagagtag ggcagagttt
      361 ccttcaagac cgccgccacc gagtctctga attcttttga ctgactgtag ttggtccttt
      421 ctgccggtgt catcttttcg atggccagca tgatgtcgct tatgccattg gcgttcttta
      481 tggagtcctt gatgtcattc ctgacgttgt ctctggtggc ttcgaaaatg gtattgtgga
      541 agctatccaa attctgtagt tgcttccgtc cttcttccgt ggctctgagt ttctgcagct
      601 ggttgagcct ttccaccgga ttcaggcttt ccagatgcaa agacagttct ctaccagcct
      661 taaactgctc cagattttcc gctttctcaa gcttgggtgc cagggttatc agttgttctt
      721 cattgccttc cttgaaatcc tttgccattg ccagcttttg gtccaggagc ttgagggcgt
      781 ttgtggaccg agcatcttca ccgtagcggt ctttcgcctg cgctttcagc atggcgtgca
      841 gttccgggtt ttccaccagg ctcttgatgg tggctgcgtc cagattttca atgcttttca
      901 tgaagttctg agcgctgcca tgggtgaaga atccggcatg tttatctagt tccagacctg
      961 cttgtgaccc ataggcgccc tgcaggtatt tggcttcctt tatgtccttc gagaaccagt
     1021 gcaagtccct acttttggct gcaaattcga caaagtagtt gtgtgcgtct ctggcctgtt
     1081 tcgcctcgtt tgtctggttc gtttcagaaa gaaggttact ggctgctata tgtcttgggt
     1141 cactttggtc tttcttgagg tcggaagcca gttcgaagcc caggcgaaag cgccctctgt
     1201 ctgccgcgct catgttctga aaaatctttt ccagttgctc ctgggatgga cctgttcccc
     1261 agttgttttc ttctatcttc gtgagggcgg aggtcttgcc ttccctcaca tagtctcggg
     1321 cgatggtcat gagccggccc ggttccgata gcggcgagaa gatgccgttt tcaaatttgt
     1381 acttgccgtc ggcttgtcgc ttgtactcag gcaccagggc cttctccagg cttttgtcgc
     1441 cgccttcgtt ttgaaactct tgcctggctc tctcgcttga gcggccggcg aactctttga
     1501 agagctccag attgttcatg gcgattttgt tgtttatggc aacgatattt tgtctggctt
     1561 cacccctgct atcgcccagt gcttcaaatt t
//
LOCUS       DAOQNZ010001085         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_61009_length_1590_cov_4.104886, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001085 DAOQNZ010000000
VERSION     DAOQNZ010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61009_length_1590_cov_4.104886"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1190
                     /locus_tag="PKW73_16600"
     CDS             81..1190
                     /locus_tag="PKW73_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNA74973.1"
                     /translation="MNNLHQAYIDLGIPVGSSLDAVERRYKRLVMAWHPDRYPNAEHK
                     REAEEELKKINSAKDVLKEHLKSSQHRETGDCECRSGAAEQAKHRQPGSSGPGRKAKT
                     TAETRQEEFEAQFKDQERANRAEEEERRKAEQNEKKAHEESVKTAFSQESQRNTESLR
                     WKICIGAAAVSVLLMFIPGLAYLIEQPILAGQNVIYGQHGENEQSRKLAEEWRSYQND
                     KAQVATTLLPQLSSGSDTTSWRPPFVSNNASADYFSRQAYQQLVVKVEEQKKQHAMDV
                     YKARGEVDRYQKAIERSQSVLADCAAKLADPNAAFTDQRITLADQADHRRYLLENEEN
                     LRLAQKRLDELEAESPGAVPQEPRFGPAPKPLQQR"
BASE COUNT          457 a          377 c          448 g          308 t
ORIGIN      
        1 gaccaccagg agcagcggga gcaggcgatg cgggatgaaa ttgctgcatg gactaggttc
       61 aaggaggcaa acaacctcaa atgaacaact tgcatcaggc ttatatcgac ttaggcatcc
      121 ccgttggctc ttcgctggac gcagtagaga ggcgctacaa gcggctggta atggcctggc
      181 atccggaccg ctatccaaat gcggagcaca agagggaagc ggaagaagag ctgaagaaaa
      241 ttaatagcgc aaaagacgtg ctcaaagagc atctaaaaag cagccagcac agagaaacag
      301 gtgactgtga gtgccgctca ggggcggctg agcaagcaaa gcaccgtcaa ccaggttcta
      361 gcggaccagg gcgtaaggca aagaccacag cagagacgcg gcaggaagag tttgaggctc
      421 agtttaagga ccaggagcgc gctaaccggg ctgaagaaga ggagaggcgg aaggcggagc
      481 aaaacgagaa gaaggctcac gaagagtctg ttaagaccgc gttcagtcaa gaaagccaaa
      541 ggaatacgga gtctctgcgc tggaaaatct gtattggtgc agctgccgtc tctgtacttc
      601 ttatgttcat cccagggctt gcttatctca ttgagcagcc aattttagct gggcagaatg
      661 tcatatacgg tcagcacggc gaaaatgagc aaagccgcaa gcttgctgaa gaatggcgta
      721 gctaccagaa cgacaaggca caggtggcaa cgacacttct gccgcaacta tccagtggaa
      781 gcgacacaac gagctggcgg ccgccgtttg tatcaaacaa tgctagtgcc gactattttt
      841 caaggcaggc atatcagcag cttgtcgtga aagtggaaga gcaaaagaag cagcacgcga
      901 tggatgtcta caaggcaagg ggcgaagttg ataggtatca aaaggccatt gagcgtagcc
      961 agagtgtgct tgccgattgc gctgccaagc tggcggaccc taatgcagcc tttactgacc
     1021 agcggataac actggcagac caggcggatc accgccggta cttgctagag aatgaggaga
     1081 atctgaggct agcccagaaa cgccttgacg aactggaagc ggagagtccc ggcgccgttc
     1141 ctcaggagcc gaggtttgga ccagccccga agcctcttca gcagaggtga gcacaacatc
     1201 ttgcattcgg tagaaccaaa cagtgggttg gtttcgaact ttgaacgtgc aaacaccgtg
     1261 gactgatgga gcagtcactg aaagcaatct atggtttgaa agccgcattg gcctttgaat
     1321 cgctcgggct acattccccg ccgcttgcgg cgcgccgaat aggaactcaa gagagtaaac
     1381 aatcaaggat accccgctgc ttgtgccggg tcggttcatt cttactttgg accgcatcta
     1441 aaaattgttt ctgtgttact tactctttgc caagcttgtt ccacaaacag cttggcttct
     1501 ttaagcattt ccaatttgtc aaaacatgcg attgtaagaa ccgaaaaaat ccatgctgtt
     1561 ggaactgaag aaacaaaatc aaacacagtt
//
LOCUS       DAOQNZ010001086         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_61011_length_1590_cov_3.910749, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001086 DAOQNZ010000000
VERSION     DAOQNZ010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61011_length_1590_cov_3.910749"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PKW73_16605"
     CDS             complement(<1..736)
                     /locus_tag="PKW73_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74974.1"
                     /translation="MKGEASLPVREFTWSNQRQMDQSSNAGLGIPLGLRQFFQLRKSE
                     YQTEPDKSDSKANVKVENFLNQTPVNDKEKRSPAEEKDLEQSREALKNAVSSEDRLSK
                     ALHLARLYQHLRYIDEAKKATELALEIDPHNLVARQLFKELERMHPADISIAALPITV
                     PNAVTKSALRNRIIGLSGGKVAVVGDLLIDELLEGKPERISREAPVLILEHVDTVHIP
                     GGAANTASNVVALGGQCMAVGVCGADE"
     gene            1351..>1590
                     /locus_tag="PKW73_16610"
     CDS             1351..>1590
                     /locus_tag="PKW73_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74975.1"
                     /translation="MIQRRSSFLKAVLLVAPAFFSFTLETRADDPNNQLRPGLGPNNS
                     TKSIRPNRIRKDRKAANAMARYHWLDRAAMANPQLI"
BASE COUNT          363 a          427 c          385 g          415 t
ORIGIN      
        1 attcatctgc cccgcaaaca ccaacagcca tgcactgtcc gcccagagca acgacgttgc
       61 tggcggtatt ggctgcgcca cctgggatat gcacagtatc gacgtgttcc agaatcaaaa
      121 ccggcgcctc tcgagagatt cgctcaggct taccttcgag caattcatcg atgagaagat
      181 caccgaccac agcaaccttg ccgccggaaa ggccaattat gcgattgcgt agcgccgact
      241 tcgtgaccgc atttggcact gtaatcggta aagcggcaat gcttatatct gctggatgca
      301 tccgttctaa ttccttgaac aattgtcgtg ccaccaaatt gtgaggatcg atttccagag
      361 cgagttcagt ggctttttta gcctcatcaa tatagcgtaa atgctgataa agcctcgcta
      421 gatgaagagc ttttgatagt ctgtcttccg agctgacggc gtttttcaaa gcttcacgag
      481 actgctccag gtccttctct tcggcgggag agcgcttttc tttatcatta actggagttt
      541 ggttcaaaaa attctcaacc ttgacattcg ccttcgagtc cgatttgtca ggctcggttt
      601 ggtattcaga ctttctcagc tgaaaaaact gacgcaaacc cagtggtatg cctagaccgg
      661 cattagagga ctggtccatc tgacgctggt ttgaccaggt aaactcccga acaggaagac
      721 tggcctctcc cttcaaaacc ctgtctctga agggctcgga gcctgcttcg cgcgacaatt
      781 ctctgcccag ttcctcggaa agacctaagc cccgagaaag cgcgcccctg ccaagttttc
      841 ccgatatata cggagtcagg tcattgttga gacgggcgag tttctgctct tcagtcccgc
      901 cctggtcttt acttgcggca ctgtgagctg ccggcaaacg gtcaccccga gaaaccatgt
      961 ctccaaggga aagcccttgc tcagaggact tttcaggggc aatatccccc ggtcctttgt
     1021 tttcgacttt atcgggctga ttgcccgtcg gtccttgatt gctcactgtg ctcacggggt
     1081 tgaggctctc ctgcggatcg aatgagctgg aatggcttgg gggaatgagc agcgaaaagt
     1141 ctcataaatt cgggatttca gcgtgccgtt ccaaacgtat tgtacctaaa ggtgcccctg
     1201 ctgggggctt acaagctttg tggcgctgcc gctcatttgt tctggtgctg tttactatgg
     1261 cctgtagctc aacaaatggt taaaatattg aagatcgttc tttccttccg gtattgaact
     1321 ttccatttac caaagccgct cgaggtcgag atgattcaac gccggtcgtc gtttctaaaa
     1381 gcagtgctgc ttgtcgcgcc tgcctttttc tcctttactc ttgaaaccag agctgacgat
     1441 cctaataatc aactgcgacc gggtttaggt ccaaacaact cgaccaaatc gataaggccc
     1501 aaccgtattc gcaaagacag aaaagctgcc aatgccatgg ccagatatca ctggttggac
     1561 cgagcggcaa tggccaatcc tcaactgatt
//
LOCUS       DAOQNZ010001087         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_61023_length_1590_cov_3.335505, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001087 DAOQNZ010000000
VERSION     DAOQNZ010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61023_length_1590_cov_3.335505"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1590
                     /locus_tag="PKW73_16615"
     CDS             <1..>1590
                     /locus_tag="PKW73_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74976.1"
                     /translation="LEEDPAFYEKFSKLIQQAIDDFRAKRISDLEYLNKVCEIKDAVV
                     NHELIWRNFHSSWLDGTTTKFYRPLISVFMVSDYVLFNKDGLGFHITNLLFHLASTVA
                     IFFIASHLAAETMRLAKGADGAALEGAIPDASFQRLWPFCAALMFGLYPLHPEAVSWI
                     TGRVDAVVTAFITLSFWCYLAGRRSKNILIQSLCYIFLVLALLSKEMAITLPAVFILF
                     EFFFPVRLSARGFSSRALLRRVTDALAPTAPFLLVIAVYFVVRLLALGTFVGGYDDSL
                     FFISDWKNFIYVWICGLRLFIEPFNRELVSVRSWPVKLWDLSLALAALLALGNLLFFR
                     PLTRLYVFLAGWLVLSLLPVYKVFAIADDLQGSRLAYLATVPLAMLLCLGVIGLGKRR
                     KALDKGFRLIHGGLFCATAFVLLNLNNQAWVEGGKTANAIRQALSELYRKVEGDPQVL
                     FVGLPDQEHGAYICRNALPGMTRAPQLERDVLNAIMVDRFEPIFPFAYVKESLFQDRD
                     KIKIFRFDRSGSCFQPVDLGG"
BASE COUNT          286 a          486 c          400 g          418 t
ORIGIN      
        1 cctggaagag gaccccgcgt tttatgaaaa attctccaag ctcatccagc aggctattga
       61 cgacttccgc gccaaacgta tctccgatct agaatacctc aacaaggtct gtgaaatcaa
      121 ggacgccgtg gtcaaccatg agcttatctg gcggaacttc cacagttcct ggctggacgg
      181 caccaccacc aagttctatc gccccctcat ttcagtcttt atggtttccg actatgtctt
      241 attcaataaa gacggactgg gctttcacat caccaatctg ctctttcacc tggcttcgac
      301 ggtggcgatc ttcttcattg cctcccatct ggctgcggag accatgcgcc tggccaaggg
      361 agctgacggt gcggcgctgg aaggggccat acccgatgcc agttttcaga ggctctggcc
      421 tttctgcgcg gccctcatgt ttgggctcta tcccctgcat ccggaagccg tctcctggat
      481 taccggacgg gtggacgcgg tggttacagc cttcattacc ctttctttct ggtgctatct
      541 ggccggccgc cgcagcaaga atatcctgat tcagagcctg tgctacatct ttctagtgct
      601 ggccttgctc tccaaagaaa tggccataac actaccggcg gtgttcattc tcttcgaatt
      661 cttcttcccg gtacgcctca gtgccagagg gttttccagc cgagccctgc tgcgaagagt
      721 gacggacgcc cttgctccca cggcgccctt ccttctggtc attgccgtct actttgtcgt
      781 gcggcttctg gcgctcggca cattcgtggg aggttatgac gattcgctct tctttatttc
      841 cgactggaag aactttatct atgtctggat ctgcggtctg agactgttca tcgagccgtt
      901 caaccgggag ttggtgagcg tgcgctcctg gccggtcaaa ctctgggact tgagtctagc
      961 cctggcagct cttcttgccc tgggcaattt actctttttc cgccctctca ccagactcta
     1021 tgtttttctt gccggctggc tcgtcttgag cctgctgccg gtctacaaag tttttgccat
     1081 cgccgacgac ctgcagggca gccgtctcgc ttatctggcc accgtgcctc tggccatgct
     1141 tctctgcctg ggggtaatcg ggctgggcaa aagacgcaag gctctagaca aaggttttcg
     1201 cctcatccat ggcggtcttt tctgcgccac agcctttgtc ttgctcaatc tcaacaacca
     1261 ggcctgggtg gaaggcggca aaacggccaa cgccattcgc caggccctct ccgagcttta
     1321 ccgcaaagtt gaaggtgatc cccaggttct ctttgtgggg ctacccgatc aagagcacgg
     1381 cgcctatatc tgccgcaatg ccctgcccgg catgacccgc gccccgcaac tggagcggga
     1441 tgtattgaac gccatcatgg tcgatcgctt tgaacccatt tttcctttcg cttacgtgaa
     1501 agagtccctc tttcaagacc gggacaaaat caaaatcttt cgtttcgacc gctccggcag
     1561 ttgcttccag cctgtggacc tgggcggcat
//
LOCUS       DAOQNZ010001088         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_61209_length_1588_cov_2.706458, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001088 DAOQNZ010000000
VERSION     DAOQNZ010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61209_length_1588_cov_2.706458"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..1198)
                     /locus_tag="PKW73_16620"
     CDS             complement(413..1198)
                     /locus_tag="PKW73_16620"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA74977.1"
                     /translation="MPDLSVIIPAYNEERRLPATLASVYGYLERTLQETGRTYEILVV
                     DDGSHDGTCEVVEEFARHQVQSESDDEQEKVRLVSYAPNQGKGFAVRTGMLKARGDYL
                     LMNDADGSSPIEEVERLLEGMKPDYKVVIGSRAKEDPTAKVNALFYRKYIGNTFNLIV
                     QSLLLLGVKDTQCGFKLFERNAAQDIFSVARLNGYAFDVELLYIARLRGYKLHEVAIN
                     WNNMEGSKVNVLTDSPRMLMEVLGITIGAWFGTYKRKIADSGS"
     gene            complement(1241..>1588)
                     /locus_tag="PKW73_16625"
     CDS             complement(1241..>1588)
                     /locus_tag="PKW73_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74978.1"
                     /translation="RGLNRITLPGPLETYKMALGNTMQSTAMATGFIGAIETTNSWNA
                     GSAYDPKNRISPFSPANSSPENLKTYQSRLRAENRRIPYSLMDYDRMRGSLQSRFDPL
                     STLALPVEQVEGK"
BASE COUNT          406 a          394 c          389 g          399 t
ORIGIN      
        1 gagaatagta ttatatcatc cggcaattag tattacagta tccggcaatt agtagtaaga
       61 cgtccggcag ctattgattg gcaaccaggc gctatgtagg caaagtacct gctgcgagcc
      121 aatcccatgc cttgagcttc aaaatagctt ccggcgcatc agcagtgctt ggctggagcc
      181 agttgcgcta cctgaatcga cctgttttcg acagcagctc tattcttgga aggtgcaaac
      241 aaggcatacc acactatcga tatattaact cccatatttg tccaacccgt gtctgtggaa
      301 tcgagcaatg ggtagttgtg cagagactgc tcctagaaat ttgccctaaa ttccttgcaa
      361 attgtgacca atagcaaggt tcgcagcgaa tctaatattc tctgtaccat ccctaggagc
      421 cggaatcggc tattttgcgc ttataagtac cgaaccaggc accaatggta ataccaagca
      481 cttccataag catgcgggga gagtcggtta aaacattcac cttggaacct tccatgttgt
      541 tccaattaat ggcgacctca tgcagcttgt agcctcgtag cctggctata tagagcaact
      601 ccacgtcgaa ggcataacca ttcaggcgcg ccaccgagaa aatgtcctgg gcggcattgc
      661 gttcgaatag tttaaagccg cactgggtgt ctttaacacc aagaagaagc agcgattgca
      721 caatcaagtt gaaagtattg ccgatgtatt tgcggtagaa aagagcattt accttagcag
      781 tgggatcttc cttggcacga gaaccaatca ctactttgta atcaggcttc ataccttcca
      841 gcagccgctc aacttcttca atgggtgagc tgccgtcggc atcattcatc aggagatagt
      901 cgccccgcgc cttgagcatg ccggtgcgca cggcaaagcc cttgccttga ttgggtgcat
      961 aagataccag tcgaaccttt tcttgttcat cgtcgctttc agactgcacc tggtgccggg
     1021 cgaattcctc cactacttca caagtgccgt catgactgcc gtcgtctaca acgagaatct
     1081 cataagtgcg gccggtctcc tgaagagttc tctctaaata gccgtagacc gatgcaagag
     1141 tagccggcaa gcggcgctct tcattgtagg cgggaatgat gaccgataaa tctggcatct
     1201 aaaagagagg aacctgaaag gagcactcta tctaaatacc ctatttccct tctacttgtt
     1261 caacaggtaa agcgagagtt gacaaaggat cgaagcgaga ctgcaaacta ccgcgcattc
     1321 tgtcgtaatc catgaggcta tacggaattc tacggttttc agctctcagg cggctctggt
     1381 aggtcttgag attctcaggg gagctattgg cagggctgaa aggactgata cggttcttgg
     1441 gatcataggc ggaaccggca ttccaggaat tagtggtctc gatagcaccg atgaaaccgg
     1501 ttgccatagc tgtactctgc atggtgttac ccagggccat tttgtaggtc tcaagcggtc
     1561 cgggcagggt tatgcgattc agacctct
//
LOCUS       DAOQNZ010001089         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_61586_length_1583_cov_2.956806, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001089 DAOQNZ010000000
VERSION     DAOQNZ010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61586_length_1583_cov_2.956806"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1136)
                     /gene="cheB"
                     /locus_tag="PKW73_16630"
     CDS             complement(36..1136)
                     /gene="cheB"
                     /locus_tag="PKW73_16630"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNA74979.1"
                     /translation="MKNDGKTKVLVVEDSITAQKLLLSIFNQDPALEVIAVAGSGEEA
                     LKILETVAPDVVTMDIHMPGMNGLDVTRKIMESKPLPIVLVSESCLVQDVERAFQIIE
                     AGALSAVAKPIGTGAGFQSAANRLKQTVKDMSQVKVVRRWPKAQASESDKRTDLKKID
                     LPSAGDLSVVAIGASTGGPNAVVEILSKLNKDFPLPLVLIQHIAPGFLGGMVEWLSQT
                     LPLKVAIPEEGEQLLPGTLYLSKEGKHMALTETLKVKYSGNGPIAGHRPAVSYLFESL
                     SVNLGARALGILLTGMGKDGAKELLAMRRAGSLTIAQDQASSVVHGMPGEAITLGAAS
                     HVLNPQEIGELLKRLAGKKVLKRNKTAEETRG"
     gene            complement(1133..>1583)
                     /locus_tag="PKW73_16635"
     CDS             complement(1133..>1583)
                     /locus_tag="PKW73_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA74980.1"
                     /translation="GNSMLSNLLDNLSGAEEPQGLALVTSKMNPTTILVVEDSITSRI
                     LLKNIFESAGYIVKMAVDGQEGLKQMRHMRPDLVVCDVEMPVLDGLGMVKAMREDQQL
                     KDLPVILVTSLASETDRKQGLNAGANAYFIKGDLDQVGLLETVRRLT"
BASE COUNT          320 a          430 c          411 g          422 t
ORIGIN      
        1 cttccaccag gagaatggtc gggactaaat ttggattatc cacgtgtttc ttccgccgtt
       61 ttgtttctct tcaagacttt cttgccggcc aggcgcttca gcaattctcc tatttcctgg
      121 gggttgagaa catggctggc agctcccagg gtgattgcct cgcctggcat gccatgcacc
      181 accgaactgg cttgatcttg agcaatagtg agggagccgg ccctgcgcat ggctaagagt
      241 tctttagcac cgtctttccc catgccggtg agaagtatgc ccagggctct tgccccgaga
      301 ttaactgaga ggctctcaaa tagataggat actgccggtc tgtgtcctgc tatgggaccg
      361 ttgccggagt actttacctt gagcgtttcc gtgagagcca tgtgcttgcc ttccttgctc
      421 aggtagagcg tgcctggcaa taactgctca ccctcttccg gtattgccac ctttaacggt
      481 agggtttgag aaagccattc caccatgccg cctagaaagc ctggtgcgat atgttgaatg
      541 agcaccaggg gcagtggaaa gtctttattc agtttgctca aaatttctac cacggcgttg
      601 ggaccgcctg tggaagcacc tatggccacg acgctcaggt cgcctgcact gggcaaatca
      661 attttcttca aatcggttct cttatcgctt tcgctagcct gcgcttttgg ccagcggcgc
      721 accactttca cctgggacat atctttgaca gtctgcttga ggcgattggc cgccgactga
      781 aagcccgctc ccgtgccgat tggcttggct actgccgaga gagcgccggc ctcgatgatt
      841 tgaaaggctc gctctacatc ctgcaccagg caactttccg acacaagtac tataggaagc
      901 ggtttgctct ccataatttt gcgggtaaca tccagtccgt tcatgcccgg catatgaatg
      961 tccatggtta ccacatccgg tgccacggtt tctaagattt tcagggcctc ttcgccggag
     1021 ccggccacgg caattacttc cagggccgga tcttgattga aaattgaaag gagtaatttt
     1081 tgtgcggtga tggagtcttc caccaccagt acttttgttt ttccgtcgtt tttcatgtca
     1141 atcgcctcac cgtctccagt aagccgactt gatccagatc gcctttgata aagtaggcat
     1201 tggcaccggc attcaagccc tgcttgcggt cagtttcgct ggccagggaa gtgacgagga
     1261 tgaccggcaa gtctttcaac tgctggtcct cccgcatggc tttcaccatg cccagtccgt
     1321 caagaaccgg catctccaca tcgcagacca ccagatcggg gcgcatgtgg cgcatttgct
     1381 tcagaccttc ttgaccgtcc acagccattt ttactatgta gccggctgat tcgaagatgt
     1441 ttttgagaag aattctggag gtaatggaat cttctacaac caggatagtg gtggggttca
     1501 tcttactagt gacgagggcc agcccctggg gttcttccgc tccggaaagg ttgtccagga
     1561 gattggaaag catggaattg ccg
//
LOCUS       DAOQNZ010001090         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_61602_length_1583_cov_2.608639, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001090 DAOQNZ010000000
VERSION     DAOQNZ010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61602_length_1583_cov_2.608639"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..812)
                     /locus_tag="PKW73_16640"
     CDS             complement(78..812)
                     /locus_tag="PKW73_16640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA74981.1"
                     /translation="MIDTHYENPRLAALYDLDSGWSIDRDFYLALAHQGGLKVLDLGC
                     GTGLLADAYARKKNTVTGVDPAQAMLAVARQKPFGAQIEWVQSPAQTYDSQKRFDLII
                     MTGHAFQVLLEDSDVLATFAVMKKHLKPGGLIVFESRNPSVNWASRWDYQMELTLPST
                     KVLESRHLLSMAGKRMRFELRYQFQDGACETLSSISELRFFSREEIEAHLKESGLYVE
                     TLLGGWRGEPFDESDSEEMIFKVRAA"
     gene            complement(813..1535)
                     /locus_tag="PKW73_16645"
     CDS             complement(813..1535)
                     /locus_tag="PKW73_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74982.1"
                     /translation="MKSTRLALALISLVLWCASAAQAQPLQEREDPLSPEVEAKEKKA
                     LDATFNADELSGSAYVEAGQKSIYVKFVSNSKRAVLVDGDSATLANVRADENSLHGYK
                     RESENPLISRNQVIKPPAKVNVPSDLLYVGGSLATIGAGPVAKDYLDRKHADAGLYYG
                     ADQQRREFAGMRFGKRLIFPGESSAGRLYFKHTPKPGSRISFKILSHPDGITIGEVVL
                     PVESPTGKPSAVPPAKTSNGLK"
BASE COUNT          330 a          414 c          416 g          423 t
ORIGIN      
        1 ccctcatcgg attcagcagg attcggagcg aataggttta aattgttgca ggccgccgat
       61 agtaactcat tcccttgtta cgctgcacgc accttgaaga tcatctcctc ggaatcgctc
      121 tcgtcaaagg gctcgcctct ccagccgccc agaagtgttt cgacatagag tcccgattct
      181 ttcagatgtg cttcgatttc ttctcttgag aagaagcgca gttcgcttat ggaagagaga
      241 gtttcacagg cgccgtcttg aaattgatag cgcagttcaa agcgcatgcg cttgcccgcc
      301 atgctcagga ggtgtcttga ctctaacact tttgtagaag ggagagtcag ttccatctgg
      361 tagtcccagc ggcttgccca gttcacagac gggttgcgtg attcgaagac gatcagaccc
      421 ccaggcttaa ggtgcttctt catgacggca aaagttgcca gtacatcgct gtcttccagt
      481 aaaacctgga aggcatgacc ggtcatgatg atcaaatcaa agcgcttttg gctgtcatag
      541 gtttgtgccg ggctctgcac ccattctatc tgagcgccaa atggtttctg tctggctacg
      601 gcaagcatgg cctgcgccgg atctacgccg gtgacggtgt tcttctttcg ggcataggca
      661 tcggccagaa gtcctgtgcc gcagcccaga tccagaacct tcagaccacc ctggtgagcc
      721 agtgctagat aaaagtctct gtctatcgac cagccgctat ccagatcata gagcgccgcc
      781 agcctgggat tttcgtaatg agtatcaatc atttatttga gaccgttgct tgtcttggct
      841 ggaggcactg ctgatggttt gcctgtcggc gactccacag gcagaactac ttcaccaatg
      901 gtgataccat caggatggct caatatttta aagcttatgc ggctgcccgg ctttggcgta
      961 tgcttgaaat agagccggcc ggcgctgctt tctcctggaa agatgaggcg tttgccgaag
     1021 cgcataccgg cgaactcacg cctttgttgg tcggctccgt agtacagacc ggcgtcggca
     1081 tgtttgcgat ctaaatagtc tttagctacc ggacccgctc ctatggtggc tagcgaaccg
     1141 cctacataga gcagatcgga aggtacatta actttggccg gcggcttaat tacctggtta
     1201 cgacttatta gtggattctc gctttcccgt ttatatccgt gcagggaatt ttcatcagct
     1261 ctgacgtttg caagcgttgc tgagtctcca tccaccagca cggcccgttt ggagttgctg
     1321 acgaatttga cgtagatgga tttttgtccg gcttccacgt aagccgagcc tgacagctcg
     1381 tcggcattga aggtggcatc gagggctttt ttctcttttg cttccacttc cggcgagagg
     1441 gggtcttctc tttcttgcag gggctgagcc tgagctgcac tggcgcacca gagcaccagt
     1501 gatattaatg ccagtgccag tctagttgat ttcatgtctt gcttccctgg tgcaagacat
     1561 gaaatcaact agactggcac tgg
//
LOCUS       DAOQNZ010001091         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_61960_length_1578_cov_5.494419, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001091 DAOQNZ010000000
VERSION     DAOQNZ010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61960_length_1578_cov_5.494419"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKW73_16650"
     CDS             complement(<1..240)
                     /locus_tag="PKW73_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNA74983.1"
                     /translation="MSRQRLKNLPGGSNPQPLSVVIGHRGLAARFPENTLVSFAAALQ
                     AGASMIELDLRLSADGQAVVIHDATVDRTTNGTGLI"
     gene            complement(376..1260)
                     /locus_tag="PKW73_16655"
     CDS             complement(376..1260)
                     /locus_tag="PKW73_16655"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA74984.1"
                     /translation="MRETSGLRPVTSNTPKKPGRIFHLAIKEPTMFPLKDNLRCSSPA
                     TVTKWLILANVVAFIIEVTMLLSGHQDQLFGTWLMVPGKVTAAFAAGDPSLMFWATAS
                     IFSSMFLHGSFGHIFGNMVFLFVFGKGMENRIGKWNYLAFYLIAGVLAALTHWGSDPT
                     SMVPTLGASGAIAGVLGGYLLFWANATISGFYVLPAPAYVHTKAYWFLIGWFVMQFSG
                     VLSSVGPIDGAGVGAFFDADKLVHFGDGVAYWAHIGGFVAGFIMAGLAKIFMPKTDVC
                     IIPKPECDAPEDHNKGSK"
BASE COUNT          362 a          393 c          500 g          323 t
ORIGIN      
        1 aatcagtccg gtaccgttgg tggtgcgatc cacggtggca tcgtggatga ccacggcctg
       61 tccgtcagca ctcagtctca ggtctagctc aatcatagag gcaccggcct ggagggcggc
      121 tgcaaaggaa accagggtgt tctcgggaaa gcgtgcagcc agtcctcggt ggccgattac
      181 cacggagagt ggctggggat ttgaacctcc tgggaggttc ttcagtcttt gccgggacat
      241 agagacctct gcttaaaggt aaaaaagttt gccgaaagga ggaaaaaaga ccaggcaggc
      301 actagctgtt tggctgagta cctgcctggt gtgaggcaga cttgccgact tttcagccga
      361 cgtggtaagg gtggcttact tcgaaccctt gttgtggtct tccggtgcat cgcattccgg
      421 cttcggaatg atgcagacgt cggtcttggg catgaagatc ttggccagtc cggccatgat
      481 gaaaccggcg acgaagccgc cgatgtgcgc ccagtaggca acgccgtcgc cgaagtgcac
      541 aagcttgtcg gcatcgaaga aggcaccgac accggcaccg tcaatcggac cgaccgagct
      601 gagaacgccg gagaactgca tcacgaacca gccgatcaag aaccagtagg ccttggtgtg
      661 gacgtaagca ggcgcaggca gcacgtagaa gccggagatg gtggcattgg cccagaacag
      721 caggtagccg cccagaacgc cggcgatggc accggaggcg ccgagggtgg gaaccatgct
      781 ggtcggatcg ctaccccagt gggtgagcgc agccagcacg ccggcgatca ggtagaacgc
      841 caggtaattc cacttgccga tgcggttttc catgcccttg ccgaagacaa agaggaagac
      901 catgttgccg aagatgtgtc cgaagctccc gtgcaggaac atggacgaga agatggaagc
      961 ggtggcccag aacatgagac tcgggtcgcc ggcagcgaag gctgcggtga ctttgccggg
     1021 aaccatgagc caggtgccga aaagctggtc ctggtggccg gagagaagca tggtgacttc
     1081 gatgatgaag gccaccacgt ttgccaggat gagccacttt gtgacagttg cagggctcga
     1141 gcaacgcagg ttgtccttga gaggaaacat tgttggctcc ttgatagcca ggtgaaatat
     1201 tcgccctggt tttttaggtg tgttggaagt gaccggtcga agtcctgatg tttctctcac
     1261 cgcctggcgc ctgtattttc ttgccgattc aatccagagc gcggaagcgc ttcagatggg
     1321 gcaattggtc gctggggatg gcggtgattt agacttgact ggaaactcag agtgaaaaaa
     1381 gaaaataccc tttagaaata gtgctcttga ggttctcaac acaagtatta gtacctgctg
     1441 tcgtaactag acctctagcg cgactgaatt atattgacaa gcccgtgggc tgaaaaagtc
     1501 tctctggaca aggcttttgc cgtccttaag cggcttgcca ggcggtcgcg ataagcgggc
     1561 tatttcttaa ggggctgg
//
LOCUS       DAOQNZ010001092         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62044_length_1577_cov_4.596583, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001092 DAOQNZ010000000
VERSION     DAOQNZ010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62044_length_1577_cov_4.596583"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..713
                     /locus_tag="PKW73_16660"
     CDS             192..713
                     /locus_tag="PKW73_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74985.1"
                     /translation="MSRKIVTTCLTAFALFSNLSAEPCRAQEAPMLSPEQALTSVPAN
                     PQDPSAPALEKRQLSADLTLIASAKSLREFRTGASGELYQALKNIVKEGKSQIPDLEW
                     IQINGSPAGRVYATLLLSRIENTTAAEALKSLKRDDDTNVEVFAGGSKCHYTLTEIII
                     DQSSKTPVIRLLP"
     gene            812..>1577
                     /locus_tag="PKW73_16665"
     CDS             812..>1577
                     /locus_tag="PKW73_16665"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74986.1"
                     /translation="MSVSGNEIGDLLLDIGLITPEELETAHQEQCRSGERLTMVLEKL
                     GLVSNNQLKDALELQFGVNYVSLSKHPPAREIVELLPEEIRLKHKVLPIAHNGNQYTI
                     AMVNPDDLIAIDALKIQLKSGQLKKLVCTADDFEFVSQLMSAPAREAEPQTIAVVSEK
                     PVKAPAKSVSPSKVPARKHLTSLFQDDDEDDLLEPLPTPAPPPANGKEAPPPEPEPEP
                     EPTAEAAAEAVPEPEAEPDPAPKAIPSSDSDFLLKEQ"
BASE COUNT          429 a          451 c          406 g          291 t
ORIGIN      
        1 ccggcatggt aatcaaaggc tgggacgaag gcgttatggg tatgaaaatc ggcggcaaac
       61 gcaaactgac agttccgccg caattgggtt atggtgcccg cggcgccggt ggggtaatac
      121 cgcctaatgc caccttggtc ttcgatgttc aactgatcgg actcgagtaa atgaagatga
      181 ggaaagggcg gatgagtaga aagatagtca cgacttgcct gacggccttc gcccttttct
      241 caaacctttc tgccgagccg tgccgggccc aggaggcacc catgctctca ccggagcagg
      301 cgttgacgag cgtaccggca aatccccagg acccgagcgc accggcgctt gaaaaacgac
      361 aattatcagc tgatttaacc ctgattgcct cggccaaaag tctgagagaa ttcagaacgg
      421 gagcctccgg cgaactctac caggctctca aaaatatcgt aaaagaaggc aaaagccaga
      481 ttcccgatct tgagtggatc cagataaacg gcagccctgc cgggcgcgtc tatgccaccc
      541 tcttgctcag tcgtatagaa aacaccactg ccgcagaagc gctgaaaagt ctcaagcgcg
      601 acgatgatac caatgtggag gtgttcgccg gcggcagcaa atgtcactac acccttactg
      661 aaatcattat cgaccagagc agcaaaaccc ctgttattcg actccttcct tgagcggctc
      721 aagattgtac cgacacaact gctcagtcag gtttagggca aattgcagag gggcacaaaa
      781 tttatgtgct tagatagaga cgggagttac gatgtccgtt agtggtaacg aaatcggaga
      841 tttactccta gacatcggac tcatcacacc tgaagagctg gaaacagccc atcaggaaca
      901 gtgccggtcc ggtgaacgct tgaccatggt gctggaaaag ctcggtctcg tctctaacaa
      961 tcaactgaaa gatgccctgg aactgcagtt cggggttaat tatgtgtctt tatccaagca
     1021 tcctcccgcc agggaaatag tcgagcttct gccggaagaa atccgcctca agcacaaagt
     1081 tctgcccatt gcgcataacg gcaatcaata caccatcgcc atggtcaacc ccgatgacct
     1141 gattgccata gacgccctca agatacaact caaaagcggc cagctcaaaa agctggtttg
     1201 caccgccgat gatttcgagt ttgtcagtca attaatgagc gctccggcca gggaagcaga
     1261 gcctcaaacc atagcggtgg tcagtgaaaa gcctgtcaaa gcaccagcca agagcgtaag
     1321 cccttccaaa gtgccggccc gcaaacatct aacttcactc tttcaagacg acgacgaaga
     1381 cgacctgctc gagccccttc ccacaccggc gccgccaccc gcgaacggta aggaagcgcc
     1441 gccgccggag ccggagccgg agcccgaacc gacggcggaa gcggcagcag aagccgttcc
     1501 agaaccggaa gcagaaccgg accccgcacc aaaagcgata ccatcttcgg acagcgactt
     1561 cctcctgaaa gaacagc
//
LOCUS       DAOQNZ010001093         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62068_length_1577_cov_3.278581, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001093 DAOQNZ010000000
VERSION     DAOQNZ010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62068_length_1577_cov_3.278581"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1577)
                     /locus_tag="PKW73_16670"
     CDS             complement(<1..>1577)
                     /locus_tag="PKW73_16670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNA74987.1"
                     /translation="LARSCGRLGLHVYAYNSYQSIIRGGHIWLRVRIGENKVYSHGDK
                     LNVLVALNQDSIERHAGEVEEGGVVIFNSDKVKCDPALVRPGVKILGLPMAKVTEDVV
                     KEHGPLQAIMQNTVAVGAVCFLSNIGLEEPTSVLTDTFAHKGQKVIDLNVGLLKAGYE
                     FAKANCQTLTKEWQFSHKKRPFVTGNEAIALGAYAAGCKFYSAYPMTPASTILHWMAS
                     HAAKTGICVKQCEDELSVINMAIGAGIAGARSMCATAGGGFALMTEAVGMAGIMEVPV
                     VVVEVQRGGPSTGLPTKTEQADLFQVYGASQGEFPRLIVAPKCIADAYETTVDVFNLA
                     DKYQLPVLLMSDLLLSEHPETVDPEVFNSDVKIDRGEVVSEWKEDQGKFKRFKFTESG
                     ISPRALPGTANCNYVSATDDHDEESILISDMYTSPGTRRMIMEKRNRKIENLLKELPA
                     PALEGPKDADLTLVCWGSTWGVVKEAVELLNAQGIKTNMLNIKYIAPFHAKEVSEILG
                     KAKKKISVEVNYTSQMA"
BASE COUNT          359 a          412 c          430 g          376 t
ORIGIN      
        1 ggccatctgg ctggtgtagt taacttcaac cgagattttc ttcttggctt tgccgagaat
       61 ctcgctcact tccttagcgt ggaaaggagc gatgtacttg atgttgagca tattggtctt
      121 gatgccctga gcattgagaa gctcgacggc ttctttcaca acgccccagg tggagcccca
      181 gcataccagg gtcagatcag catccttagg tccttccagc gccggagccg gcaattcttt
      241 gagaaggttt tcaatcttac gattgcgctt ctccatgatc atgcggcgcg taccggggga
      301 tgtatacata tcggagatga ggatgctctc ttcgtcatga tcatcggtag ccgatacata
      361 gttgcagttg gccgtgccgg gcagagcgcg gggcgaaata cccgattcgg taaacttgaa
      421 gcgtttgaat ttgccctggt cttccttcca ctcgctcacc acttcaccgc ggtcaatctt
      481 cacatcgctg ttgaagacct cgggatcgac ggtctcaggg tgctcggaga gcaacaggtc
      541 ggacatgagc aagaccggga gctgatactt atcagccagg ttgaacacat ctacagtggt
      601 ctcataagca tcggcaatgc atttaggagc cacgatcagg cgggggaatt caccctggga
      661 agcaccataa acctggaaca ggtctgcctg ttcggtcttg gtgggaagac cggtagacgg
      721 tccaccgcgc tgtacttcca ctaccaccac gggcacttcc atgatgccgg ccataccaac
      781 ggcttcggtc atgagggcaa agccgccgcc ggcggtagca cacatggaac gagcaccggc
      841 gataccggca ccgatagcca tattgattac ggaaagctcg tcctcgcact gcttgacgca
      901 aattccggtc ttagcagcgt ggctggccat ccagtgcaag atggtagagg cgggggtcat
      961 ggggtaggcg ctataaaact tacaaccagc cgcataggca ccaagggcga tggcttcgtt
     1021 accggtaacg aagggacgct tcttgtggct gaactgccat tccttggtga gagtctggca
     1081 attggctttg gcaaactcat aaccggcttt caagagtccg acgttaaggt cgattacttt
     1141 ctgaccttta tgagcgaagg tatcggtaag aacagaagta ggctcttcca gtccgatatt
     1201 ggaaaggaaa caaacggcgc ccacggctac ggtgttttgc atgatcgctt gcagcggacc
     1261 gtgctctttc accacatctt cagttacctt ggccatgggt agaccgagga tttttacacc
     1321 aggtctgacc agagccggat cgcacttaac tttgtcggaa ttgaagatga ctacgccacc
     1381 ttcttctact tctccggcat gacgctcaat ggagtcctgg ttcagagcca ccagtacgtt
     1441 gagtttgtcg ccgtgagagt aaaccttgtt ttctccaatt cttacgcgga gccaaatatg
     1501 accgccccgg ataatggatt gataggagtt atatgcgtat acatggagac caagtctgcc
     1561 gcatgacctg gccaggg
//
LOCUS       DAOQNZ010001094         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62133_length_1576_cov_4.383958, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001094 DAOQNZ010000000
VERSION     DAOQNZ010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62133_length_1576_cov_4.383958"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKW73_16675"
     CDS             <1..222
                     /locus_tag="PKW73_16675"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNA74988.1"
                     /translation="RDPSNQVVIPDDTFTSRHHAWITFEEGDFWIEDLGSTNGTLLNG
                     HPVVKRQVLSAGDKVRVGHTELTFEVEKN"
     gene            244..1053
                     /locus_tag="PKW73_16680"
     CDS             244..1053
                     /locus_tag="PKW73_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74989.1"
                     /translation="MADAGGANRIELESQILCAALRQSWADNDVTGQVNSCSELYQLF
                     PDLVPDLRPNQTETPRNLYNVLAIDCDVPPSIVVAGFFKAAKQFLRQGSPKDRKEEYY
                     RVLDAGFILRKPRLRLSHDLIVSRGELVAAEMIPDDGTFELIIEQEVRLPQPLMVTTQ
                     PIVHQPLPMLVELMKEAQFIGAAEVQALTNQMRSFPDVALADLVLSAGYVTDSEMKSL
                     QLAEYLLSQGKINMGQFCVAMYDERMTGVKMAESLQVRGWLETQVSPYRKD"
     gene            1255..>1576
                     /locus_tag="PKW73_16685"
     CDS             1255..>1576
                     /locus_tag="PKW73_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74990.1"
                     /translation="MPSASIDAAYPRHGFRAQGRVGRLQPKLGDFNLSTPKQGAVRAS
                     SPEAFRERLLDYLSQSATLTEKALLEAGTVASTGSSSRAARMLDAWLKGRQGIDKVSR
                     STNTS"
BASE COUNT          336 a          411 c          446 g          383 t
ORIGIN      
        1 cgggacccct ccaaccaggt tgtcattccc gacgacactt ttacctcccg gcatcatgcc
       61 tggattacct ttgaagaagg cgatttctgg atcgaagatc ttggttccac caatggcaca
      121 ttgctgaacg ggcatcctgt ggtgaaacgg caggtgcttt cggccggtga caaagttcgt
      181 gtgggtcaca ccgaattgac gtttgaagtt gagaagaact aggttaaaag gtggtccagg
      241 gtcatggctg atgcaggcgg agcaaacaga attgagctgg aaagccagat tttgtgtgcc
      301 gctctcaggc agagctgggc agataacgat gtcaccgggc aggtcaattc ctgcagcgag
      361 ttgtatcagc ttttccccga cctggtgcct gatctgagac ccaatcagac cgagacgccg
      421 cgcaacctct acaacgtact ggctattgat tgcgacgtac cgccttccat tgttgtggcc
      481 ggtttcttca aggccgccaa gcagtttctc cggcaaggca gtcccaagga tcgcaaagaa
      541 gagtattacc gggtgctgga tgccggcttt atcctgcgca agccccgtct gcggttgagt
      601 cacgatctga tcgtcagtcg cggtgaactg gttgcggcgg aaatgatacc ggacgacggt
      661 accttcgagc ttattattga gcaggaagtg cgcctgcctc agccgctgat ggttacaacc
      721 cagcccatag tccatcagcc tctgcccatg ctggtggagc tcatgaaaga agcacagttt
      781 atcggtgcgg cagaagttca ggctctcacc aatcagatgc gcagtttccc cgatgtggcc
      841 cttgccgacc tggttctttc cgccggctac gtgaccgact cggagatgaa gtctttgcag
      901 ttggctgagt atcttctcag ccagggaaaa atcaacatgg gtcagttctg tgttgccatg
      961 tatgacgagc gcatgaccgg cgtcaagatg gctgagtccc tgcaagtaag gggctggttg
     1021 gaaacgcagg tctctcccta tcgcaaggat tagggcgtta tttagccgtc ggtccaattt
     1081 tatatgtgtc gtaagtgcag tagggaaaga ctaaaaccta ctcaggtact tgatttcgcc
     1141 gggatttcaa gtcttttgat gttacaatct atcggcttga ttgtggactt tgcaagccca
     1201 cggtccggtt ggtaataccc tcaggaatct cctggttgtc agggcttttc ttccatgccc
     1261 tccgctagca tagatgccgc ttatcctcgc catgggtttc gagctcaggg gcgagtcggt
     1321 cgcttgcagc ctaaattggg cgacttcaat ctttccaccc ccaagcaggg tgcggtccga
     1381 gcctccagtc cggaggcatt tcgtgaacgg ctgctggatt atttgagcca gagcgccact
     1441 ttgacagaga aggctctcct ggaagccgga acggttgcct cgaccggttc cagttcacgg
     1501 gcggcacgta tgctcgatgc ctggttgaaa gggcggcagg gcattgataa ggtcagtcgc
     1561 tccacaaata ctagcg
//
LOCUS       DAOQNZ010001095         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62234_length_1575_cov_3.515789, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001095 DAOQNZ010000000
VERSION     DAOQNZ010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62234_length_1575_cov_3.515789"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKW73_16690"
     CDS             <1..638
                     /locus_tag="PKW73_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74991.1"
                     /translation="ASLYNQLCAVAGSQCQYFPGAIELLKDLQQAGVQNFISSALEQE
                     VLDHWLTSEQGLALAPHLAEVLGKREAFAKGKEHFAHVAKLTRRQTPQPILYVADARQ
                     EILQAKSCQTSFNLFIVGFAHCLKKEKFARADELILTSIKGHRHLIESPLGVYLNNEA
                     GRADKQGALLDLNILELPAESGLANAGTDLIVEGAGSEIFNLLRQKLQACS"
     gene            complement(633..1265)
                     /locus_tag="PKW73_16695"
     CDS             complement(633..1265)
                     /locus_tag="PKW73_16695"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HNA74992.1"
                     /translation="MTLSQDVSTPTVMDILRQSTQDLHNDAEGQDFQKSLGSGTVNVP
                     SYCNYLAQLYMMHSHLNKLLEGAAGDPRVSRVLKPYHLDCRCLERDLAHFHHDPAGIE
                     PLASTSKIMDDLTAISLESPCGLLGALYVLEGSTNGAKFMAKNLRKGLNLPEESGASY
                     FDRYGDVQRQNWLKFKEDMIACNFSPDECRELVKSARRMFQAFFEIGREL"
     gene            complement(1291..>1575)
                     /locus_tag="PKW73_16700"
     CDS             complement(1291..>1575)
                     /locus_tag="PKW73_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74993.1"
                     /translation="PSADLLLYFQDDLKLAEQWVVNGRHYQLTAGAWLANMDSNRRKI
                     MPILSATYGEDRAVRWWVFWRLFFMACQELWGYSDGEEWNVCHYLFLAPP"
BASE COUNT          404 a          423 c          394 g          354 t
ORIGIN      
        1 tagcatctct ctacaaccag ctctgtgccg tagctggtag ccagtgccag tatttcccgg
       61 gggcaattga actactgaaa gacttgcagc aagccggggt gcagaatttc atcagctccg
      121 cccttgagca agaggttctg gaccactggt tgacctcaga gcaaggtctg gctctggctc
      181 ctcatcttgc cgaagttctt ggcaaaagag aggctttcgc aaagggaaag gagcacttcg
      241 cccatgtggc aaagcttacc cgcaggcaaa ccccgcagcc aatcctctat gtggctgacg
      301 ccagacagga aatcctgcag gcaaagtcct gccaaacaag ctttaacctt ttcatcgtag
      361 gctttgctca ctgcctgaag aaggaaaaat ttgcccgggc cgatgaactg attttgacat
      421 ccataaaagg gcaccgacac ctgatagaat caccgcttgg ggtctacctt aataatgagg
      481 ctggcagagc cgacaagcag ggcgcactgc tcgatttgaa tattctggaa ttacccgcag
      541 aaagcggtct ggccaacgcc ggaacggact tgattgtaga aggtgcgggc tcagagatat
      601 tcaacttgct caggcagaag ctgcaagcct gttcatagct cccgaccaat ttcaaagaat
      661 gcttgaaaca tacgtcttgc cgatttaacc aactcccgac attcatccgg gctgaagttg
      721 caggcaatca tgtcttcctt gaacttgagc cagttttgcc gctgcacatc gccatatcga
      781 tcgaaatagg aagcaccgct ttcttcaggt aagttcagac ccttacgcaa attcttagcc
      841 atgaacttgg caccattggt ggaaccctcc agaacataaa gcgctcctaa aagcccgcag
      901 ggagactcaa ggcttatggc ggtcaaatcg tccatgatct tgctggttga ggccaggggc
      961 tcaatcccgg cagggtcatg gtgaaaatgg gccagatctc tttcgagaca gcggcagtcg
     1021 aggtgataag gcttgagcac acggctgacg cgcggatcgc cggcagcacc ttccagaagc
     1081 ttgttcagat gactgtgcat catataaagc tgcgccagat agttgcaata agaaggcacg
     1141 ttcactgtgc cgctcccaag agatttctgg aaatcctgcc cttccgcgtc attatgcaag
     1201 tcttgggtcg actggcgcag tatatccatg accgtggggg tgcttacatc ctgggaaagc
     1261 gtcattcaat tacctcctgg gtaagactct tcatgggggc gccaggaaga gataatggca
     1321 tacattccat tcttccccat cggaatatcc ccataactcc tggcaagcca tgaagaaaag
     1381 acgccagaat acccaccatc taacagcccg gtcttctccg taggtggcgc tcaaaatcgg
     1441 cataattttc cgccgattgg aatccatatt agctaaccag gctccggcag tcagttgata
     1501 atgcctgccg tttaccaccc attgctcggc cagcttgaga tcatcctgaa aatagagcag
     1561 cagatcggca gaggg
//
LOCUS       DAOQNZ010001096         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62315_length_1574_cov_4.113232, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001096 DAOQNZ010000000
VERSION     DAOQNZ010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62315_length_1574_cov_4.113232"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PKW73_16705"
     CDS             <1..1281
                     /locus_tag="PKW73_16705"
                     /inference="COORDINATES: protein motif:HMM:NF027856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74994.1"
                     /translation="TVATRLAGNVTVREENARAALEVMSRFALHPRWLVYLPPTMSPS
                     ETSKQEGFLERPEEALAYYRGNGVSQVVLEEKHMGSRAVVIVGRDEAAIARRFGLSGG
                     AATGATGSAIGGAGGVDGDGEGRLGVIYTRTGRAFFADDSQSRALERQLLQRVSKALT
                     EAKLFDHYQSDWLILDCELMPWSQKAQALLKEQYAAVGACADMVLGERTQLLSAALAN
                     GLPVENLLAIEKDKHSNALKFVDSYRNYCWPVKQLSDLKLAPFQIIASEGVCHMGVDH
                     LSQMETLARLSAIDPEVFRATAYELVDTDSDEGIAKAALFFEKITATGEGMVVKPLAA
                     IVRGPKGYVQPAVKCRGREYLRIIYGSDYTRPENLERLRSRGLGAKRSLAGREFALGL
                     EAVERFVRREPLYRVHECVFSLLALESEPVDPRL"
BASE COUNT          321 a          379 c          478 g          396 t
ORIGIN      
        1 actgtcgcta ctcgtctggc cggtaacgtg acagtgcggg aagaaaatgc cagagcagca
       61 ttggaagtga tgagccggtt tgcattgcat ccgcgctggt tggtctattt gcctccgacc
      121 atgtcaccca gcgagaccag caagcaagaa ggctttcttg aaagacccga ggaggcattg
      181 gcctattacc ggggtaatgg cgtcagccag gtagtgctgg aagagaagca catgggttca
      241 agggctgtgg taatcgttgg tagagatgaa gcggcgattg ccaggcgctt tggtttgtcg
      301 ggtggtgctg ctactggtgc taccggcagt gcgattggtg gtgcaggtgg cgtggatggt
      361 gacggcgaag gtcgccttgg tgttatctac actcgcactg ggcgagcttt ctttgccgat
      421 gatagtcagt cgagggccct tgaaaggcag ctattgcaac gtgtttcaaa ggctttaact
      481 gaagccaaac tctttgatca ttaccagtct gactggttga ttcttgactg cgaactgatg
      541 ccctggtctc agaaagcgca ggctcttttg aaggaacagt atgcggctgt gggagcttgc
      601 gccgacatgg tgctcggtga acgcacgcaa ttactttcgg cggccctggc taacggcttg
      661 cctgtagaga acctccttgc catagagaaa gacaagcact ccaatgcctt gaagtttgtc
      721 gactcttatc gaaattactg ctggccggta aagcagctta gcgatctgaa actggctcct
      781 tttcagataa ttgccagtga aggggtctgc catatgggtg tggatcatct atcacagatg
      841 gagactcttg cccggttgag cgccattgat ccggaagtgt tccgggctac tgcctatgag
      901 ttggttgata ccgatagtga tgaaggtatc gctaaggcag cgttgttctt tgagaaaatc
      961 accgctactg gtgaaggtat ggtggtgaag cctctggctg ccattgttcg cggtcccaag
     1021 ggctatgtac agccggcggt taaatgccgt gggcgagagt atctgcgcat catttatggt
     1081 tccgattata cgcgccccga aaatctggag cggctccgct caagggggct tggtgccaag
     1141 cgctccctgg ccggacgtga attcgccctg gggcttgagg ctgtggagcg ctttgtcaga
     1201 agagagcctc tctaccgcgt gcacgaatgt gtcttctcgc tgctcgccct tgagagcgaa
     1261 ccggtagatc cgcgcttgta gaaggccgac tggcgggcat attgcttgtg gagttgcttg
     1321 ggagaagatt tattttcact ctacctactg tatttgacgt tgcgccacgg agccatcctt
     1381 tatcagcggc tccgcggcgc tatcttttag cagcggctct ggtctcactt ttgtatggtt
     1441 ccggctgtct tttctacgac cttccggttc tcgcacagtc cccaactaat acctcaaaag
     1501 gcggggcaag tgccgctaaa gacggcgcaa gtgccgctaa gggtggggcg attgccgcta
     1561 aagacggcgc aagt
//
LOCUS       DAOQNZ010001097         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62413_length_1573_cov_3.096179, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001097 DAOQNZ010000000
VERSION     DAOQNZ010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62413_length_1573_cov_3.096179"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKW73_16710"
     CDS             complement(<1..521)
                     /locus_tag="PKW73_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74995.1"
                     /translation="MEAPSSTQPLNSYLQKLEFPGWQPASFKSWNSQGDTPAVEAQSD
                     LLAGEEQNGVLQEFKIVYQARKTLSRGQRNIYIDVYQFENSDFALAAYYYLRKGASTI
                     VKRGDATSEDDASISFAQGRCFVSISASEDDDESKDLVRRIADGIGARLNQTAPSVPP
                     KVLQQLPPLEKLSG"
     gene            complement(662..1069)
                     /locus_tag="PKW73_16715"
     CDS             complement(662..1069)
                     /locus_tag="PKW73_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74996.1"
                     /translation="MFSRRIGSIWALILVVVLSTFSLSGCVNKEQPTGVKIPEGFKIN
                     CYPKSECTRADSTPLGDNKFSYVVILKSKDDRFKIQTFYKTEIAMSGYKLLNDASQGT
                     EILMNAVNDQYRLDISILTVADQSIVSLSWRPR"
     gene            complement(1161..>1573)
                     /locus_tag="PKW73_16720"
     CDS             complement(1161..>1573)
                     /locus_tag="PKW73_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74997.1"
                     /translation="IEKGARVVDRQVDRNGSADTKKHYKEGKQVAEQVAPIGRLATDG
                     LGGVTDIAADGVKKKAIDSVVKDPEGTADAAAGAGKKALSWLSGLKDKVVGGDKAAEK
                     PAGTEGIGEVRKDDAKKAAPAADGVAEVRKKPKW"
BASE COUNT          340 a          446 c          396 g          391 t
ORIGIN      
        1 cccgacagct tttcaagagg cggtagctgc tgcaatactt tcggcggcac cgaaggcgca
       61 gtctgattga gacgcgctcc gatgccgtcg gcaatacgcc tgaccagatc tttgctctcg
      121 tcgtcgtctt cgctggcact tatggagaca aaacagcgcc cctgggcaaa ggagatgctg
      181 gcatcatctt cggatgtagc gtcgccccgc ttgacaatag tgctagcccc cttgcgcaga
      241 tagtaatagg cagccagggc aaaatcactg ttctcgaact gatatacatc tatatagata
      301 tttctctggc cgcgactgag agtttttctg gcctggtaaa caatcttgaa ctcctgcaag
      361 acaccgttct gttcttctcc ggccagtaaa tcgctttgag cttcgacagc cggggtatca
      421 ccctgactgt tccaactttt gaatgacgcc ggttgccaac cgggaaactc cagcttctgc
      481 aagtaggaat tgagcggctg cgtggaagag ggtgcttcca tggccggcac gggcagactg
      541 taacacccca gggtccagag agttagaagg caagccaaag cgcccgggcc ggccctgaag
      601 agtttcgcgc cggctgggcg ccggtctggt ctgaaaggcg ccaccacggg aggtgccgtc
      661 cttagcgcgg tcgccaggaa agactgacga tactttgatc tgccacggta agaatgctga
      721 tatccaggcg atattgatca ttgacggcat tcatcaagat ttcggtaccc tgactggcat
      781 cgttcaagag tttgtacccg ctcatggcga tttcggtttt ataaaaggtc tgaattttga
      841 agcgatcatc cttgctcttg agaatgacca cgtaactaaa cttattgtct cccagaggcg
      901 tggagtcggc acgagtacat tcggacttgg gataacaatt gattttgaag ccttcaggaa
      961 ttttcacacc ggtgggctgc tctttgttga cgcaaccaga gagagaaaag gtgctgagaa
     1021 ccacgaccag aattagagcc cagatagagc caatacgacg actgaacata gagatgcata
     1081 cctcaacttt cccatctcaa atcatagcgc agccagagcc aactttgtgc ttctgtcggg
     1141 tttgaggttc cagtttgtgc ctaccactta ggctttttgc gcacttccgc cacaccgtcg
     1201 gcagcaggag ccgctttctt tgcatcatcc ttgcgcactt ccccgatacc ttctgtgccg
     1261 gcaggttttt cggctgcttt atcgccgccg accaccttat ctttcaagcc actcaaccag
     1321 ctcaaggctt tcttgcctgc gccggcggct gcatcagccg tgccctcggg atccttaacc
     1381 accgaatcga tggctttctt cttgactccg tcggcagcaa tatcggttac gccacccaga
     1441 ccatcggtgg ccagacggcc gattggcgcc acttgttcag caacctgctt gccttctttg
     1501 tagtgtttct tggtatcggc actaccattc ctgtctacct gcctatccac aaccctggcg
     1561 cctttctcga tta
//
LOCUS       DAOQNZ010001098         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62575_length_1571_cov_2.886544, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001098 DAOQNZ010000000
VERSION     DAOQNZ010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62575_length_1571_cov_2.886544"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="PKW73_16725"
     CDS             <1..1246
                     /locus_tag="PKW73_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA74998.1"
                     /translation="WPYAALGLAGVYLSGVIDPSKHRNMRTTFLLGISVALVTSKPEH
                     LPEVAAAFSAGMLAWKVMSNFTSPEQARLDDVAASFAYLASMACVSTTLSTSGRNGDI
                     IAGAFATAVLLNLMQRPFMHDDKLIVKRLVLTIAGGLGMLVVLTKGLNALTFTNLAVL
                     VGAGFGSMYALDALGRMAQDERLSDGERAGKHIKALLIVGIFTLLATRLFGNLGLAAI
                     SACVMVSAFTQLPACAALFFGARLMEQAFAAAQVSNVTGINLQHSYVSAAQYLGFFVV
                     MAVMVMIRYNKERRIDAAAIALLATFGTGLASFFLHAEPAAGYLVALGVAGVVGAVLL
                     QTFFGRSPEDEARCLSVILIPSLAGSCAVLSGGLVASGLTASMEERLTTLAVLAGLTV
                     VLLLISHFFGKSTTDSVTVAGD"
     gene            complement(1211..>1571)
                     /locus_tag="PKW73_16730"
     CDS             complement(1211..>1571)
                     /locus_tag="PKW73_16730"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNA74999.1"
                     /translation="YQLALLSGLDIVERSNHVRPVSSVAPGLDATVWYGSQDLRLKNN
                     LPYPVEISSRIEGNFAYMKILGKAGLKPIKRRLERKEIARDNRTCLVEVYLLSSETEN
                     GSPETRLVSRDCYRIGR"
BASE COUNT          268 a          423 c          443 g          437 t
ORIGIN      
        1 ctggccttat gcagctctgg gacttgccgg cgtctatcta tccggtgtca tagatccttc
       61 caagcatcgt aatatgcgca ccactttttt gcttggcatc agtgttgccc ttgtcaccag
      121 taaaccagag cacctccctg aggtggccgc tgccttcagt gccggcatgt tggcctggaa
      181 ggtcatgtct aactttacct cgcctgagca ggctcgattg gatgatgtgg cggcgtcctt
      241 cgcttatctg gcctctatgg cctgtgtcag cactactctt tcgacctccg gtcgaaatgg
      301 tgacatcatc gctggtgcct tcgctacggc cgtcttactc aacttgatgc agcgaccctt
      361 catgcatgac gataagctta tcgtcaaaag gctggttctg accatagccg gcggcttggg
      421 tatgctggtg gttctcacca aggggttgaa cgccctcact ttcaccaatt tggcggtcct
      481 ggttggtgcc ggtttcggct ccatgtatgc cctggacgca ctggggcgga tggctcaaga
      541 tgagcgactc tccgacggcg agcgggccgg caagcacatc aaggccttgt tgatagtcgg
      601 catttttacc ttattggcca ccaggctctt cggcaatttg ggactggctg ccatttcagc
      661 ttgtgttatg gtttctgcct ttacgcagtt accggcatgc gctgctttgt tctttggagc
      721 aaggcttatg gagcaggctt tcgccgcggc gcaggtatct aatgtgaccg gcatcaacct
      781 gcaacattcc tatgtgagtg cggcgcagta cctgggattt tttgtggtta tggctgtaat
      841 ggtcatgatt cgctacaaca aagaacggcg cattgatgca gctgccattg ccttgcttgc
      901 cacctttggt accggtctgg ccagtttctt cttgcacgcc gaacctgccg ccggttacct
      961 ggttgccctg ggtgtggccg gtgttgtggg ggcagttctt ttgcagacct tctttggtcg
     1021 cagccctgag gatgaagcgc gctgcctctc cgtaattttg attccgtccc tggccgggtc
     1081 ttgcgccgta ctctccggtg ggctggtggc gtcggggttg accgcttcca tggaagagcg
     1141 tttgactacg ctggctgttt tagccgggct gactgtggtc ttgctcctga tttcccactt
     1201 cttcggaaag tcaacgaccg attctgtaac agtcgcggga gactagtcgg gtctcggggc
     1261 tgccgttttc agtttcagac gagagcaaat acacttccac caggcaagta cgattgtctc
     1321 tggctatctc ctttctttcc aggcggcgtt tgattggttt cagtccggcc tttcccagaa
     1381 tcttcatata ggcgaagttg ccttcgatac gggaggaaat ctccaccggg taaggaaggt
     1441 tgttcttgag tctcaaatcc tggctgccgt accagaccgt agcatcgagg ccgggggcta
     1501 ctgaactgac ggggcgcaca tgattgcttc tttccacaat gtcgaggcct gagagcaaag
     1561 ccagttgata g
//
LOCUS       DAOQNZ010001099         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62799_length_1568_cov_3.669531, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001099 DAOQNZ010000000
VERSION     DAOQNZ010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62799_length_1568_cov_3.669531"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKW73_16735"
     CDS             complement(<1..210)
                     /locus_tag="PKW73_16735"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNA75000.1"
                     /translation="MEEEYTRTEAAALTSRKVGIGEKVMLHGHIQSIRQLGQLSFLNL
                     RDGSGQVQLLVEGSELMEALKIKDIL"
     gene            complement(494..>1568)
                     /locus_tag="PKW73_16740"
     CDS             complement(494..>1568)
                     /locus_tag="PKW73_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75001.1"
                     /translation="SLDRIEQVLPKFKEAFNRLYDYGGKKPIGMIALSMGGNLSYQAF
                     TDKEIEPKVKLLLTMGTPFHGSPLFCRDWLAYTVYKRLSWPWSRIDHNLAFKLYFKKN
                     PNLQNDLTWDNADAAIPDVGKFWSKLPFGPSGKLTVENTLNERLLKVNTTPTRREKLI
                     TYGGYIVNPYLAPRAKRYLENAFMYPLFLVSTTFPAHFAREHPVLEMLNRDIANIQVP
                     KSVAKTAGTPFVYVLNDGITPVSSAIFVPPDVAKQNYVAKELDVEKLKGRLDVGRARV
                     FRNVDHLTFIDGVRPVLPLVASYQQIKDELNPQETPRDMFGWILGDIMAADAEEAARL
                     ARKTEPSAEPSKQAKDLVETSSQ"
BASE COUNT          336 a          371 c          437 g          424 t
ORIGIN      
        1 caagatatct ttgattttca gggcttccat tagctcgctg ccttccacca gaagctgaac
       61 ctgaccactt ccgtcgcgca gattgagaaa ggacagctgg ccaagctgcc ggatggattg
      121 aatatgacca tggagcatta ccttctctcc aattcctacc ttccggcttg tcagggcggc
      181 tgcttccgtc ctggtgtact cttcttccat aatttttctc ctgcgtgatt caatgcaaaa
      241 aaaacgaccg ctgactgatg tcggcggtct ctctttgctt ttgttttttt cgggctaact
      301 gctcaactca tacccgaaag aagataagac aaaaagaacc gccggggcga ttatcatttt
      361 tgtcgtcatt ggtctttgtg gtgggtctga acacggtgag gtcggtcctg aaattttctg
      421 tctaagtctt ttccatgcta agggcgtcat cgacagtggt caatgacaag ttgcggaaag
      481 taaattgaaa gttttactga ctggaagttt caaccaggtc tttggcttgc ttggacggtt
      541 ctgccgacgg ttcggttttt ctggccagac gtgctgcttc ttcggcgtcg gcggccatga
      601 tgtcgcccag tatccagccg aacatgtccc ggggagtttc ctgtgggtta agttcgtctt
      661 tgatctgttg gtaggaagct accaggggca gaaccggacg cacgccgtcg ataaaggtaa
      721 ggtgatcgac gttgcggaag accctggcgc ggcccacgtc aagccggccc ttcaattttt
      781 ccacgtctaa ttctttagcg acatagtttt gcttagccac atcgggcggc acgaagatag
      841 cgctggagac aggtgtgatg ccgtcgttca ggacatagac aaagggggta ccggcagttt
      901 tggccaccga cttgggtact tgaatattgg ctatgtcgcg attgagcatt tccaaaaccg
      961 gatgttctct ggcaaaatga gccggaaagg tcgttgacac caggaatagc gggtacataa
     1021 aggcgttttc caggtaacgc ttggccctgg gtgccagata gggattgact atgtagccac
     1081 cataggtgat gagcttctct cttctggtag gcgtggtatt aaccttcagc aggcgctcgt
     1141 tcaaagtgtt ttctacagta agtttgccgc tcggtccaaa gggaagtttg gaccagaact
     1201 tcccgacgtc cgggatggcc gcgtcggcat tgtcccaggt cagatcgttt tgcaaattgg
     1261 gattcttttt gaagtagagc ttgaaggcca gattgtggtc gatgcggctc cagggccagg
     1321 aaagcctctt gtagactgta taggccagcc agtcgcggca gaacagcggg gagccgtgga
     1381 agggcgtgcc catggtcagt aagagtttga ccttgggctc aatttctttg tctgtaaaag
     1441 cttggtaaga aagattgccg cccatgctga gggcgatcat gccgatcggc tttttgccgc
     1501 cgtaatcata cagcctgttg aaagcctctt tgaatttggg cagcacttgt tcgatgcggt
     1561 ccaggctg
//
LOCUS       DAOQNZ010001100         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62820_length_1568_cov_2.959683, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001100 DAOQNZ010000000
VERSION     DAOQNZ010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62820_length_1568_cov_2.959683"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKW73_16745"
     CDS             complement(<1..211)
                     /locus_tag="PKW73_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75002.1"
                     /translation="MSLTIEELPESIALLCSNLQAANTTQSVFGRIVRSPDTPTKLAI
                     DEGRKFVMVMDESGLKRMDELDGDAD"
     gene            complement(266..1369)
                     /locus_tag="PKW73_16750"
     CDS             complement(266..1369)
                     /locus_tag="PKW73_16750"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNA75003.1"
                     /translation="MLRWLTFAIAIWILYLVRVQLPPFIAGAALAYMLSRPVNALSAY
                     IGRTAAIVLIYLTALGAFGYVILHFAPLFAEELVALFDNRQHLVENVVNQVASLTGLQ
                     LDVTQVMAGLSEQIQGHLAEKPTEILALGHLLTHSFLSLLICLITSIYMTVDGKKLGR
                     FALRFVPENSRQEVSEMAGDIDGKLGKYLLGQAFLIAVMSLFAFLVLSFNHVHYALIL
                     SVFIGVMEIVPFIGPLIALGVVLLVAGAQLGLSDTGIVIAILYAGRMVEDYLVVPKVV
                     GHAVELPSLATIFAVLAGETIGGGLGMLLSIPVAAAVKVVLDRLLPPLPEAKDEGPDC
                     RPPTALARLNSFGKFLKERLARLIKEFAPVPFH"
BASE COUNT          383 a          418 c          422 g          345 t
ORIGIN      
        1 catcagcatc tccgtccagt tcatccattc tctttagacc tgactcgtcc atgaccatga
       61 caaacttgcg tccctcgtct atggccagct tggtcggcgt atccgggctg cgcacaattc
      121 gtccgaaaac agattgggtc gtatttgctg cttgcagatt gctgcaaagg agagcgatgc
      181 tctccggtaa ttcttcaata gtaagactca tgagcactct cccaggcgag tgtttttgtg
      241 aagttaagag cgctttgttt gccgcttagt ggaagggtac cggggcgaac tctttaatca
      301 gtcgcgccag tctttccttg aggaacttgc caaagctgtt aagccttgcc aatgcagtag
      361 gcggacggca gtcaggaccc tcatctttcg cttccggcag aggtggtagc agtctgtcta
      421 gcactacttt gacggctgcc gccaccggta tggagagcag catccccaat ccaccgccga
      481 tggtttctcc ggccagtacg gcgaagatcg ttgccagaga cggtagttca acggcgtgtc
      541 cgaccacctt cggcactacc agatagtcct caaccatgcg accggcatag agtatggcga
      601 ttactattcc ggtgtccgat agacctagtt gagcaccggc taccaatagc accacgccaa
      661 gggcaatcaa gggtcctata aagggcacta tctccatgac cccgataaaa accgagagga
      721 ttagggcgta gtgcacgtga ttgaagctca gcactagaaa ggcgaagagc gacatgacgg
      781 cgattagaaa ggcctgaccg agcaagtatt tgcccagctt gccatcaatg tcgccggcca
      841 tttccgagac ttcctgccga ctgttttcgg gcacgaagcg cagagcaaaa cgccccaact
      901 tcttaccgtc tacagtcatg tagatggagg tgatcaagca gataagcagg ctcaggaagc
      961 tatgggtgag caggtgtccc agggccaaga tttcggtggg tttttcggca aggtgtcctt
     1021 gaatttgttc gctcaagccc gccataacct gcgtcacgtc cagttgcaag ccggtcagag
     1081 aggccacctg gttaaccaca ttctcgacca gatgttgtcg attgtcgaac aatgccacca
     1141 actcttcggc aaagagagga gcaaagtgca agatcacata gccgaaggcg ccaagcgcag
     1201 tcaggtagat gaggacgatg gcggcggtgc gaccaatata ggcggaaagc gcgttcaccg
     1261 gtctgctcag catataggcc agcgcggcac cggcaatgaa gggcggcagt tgcacccgca
     1321 ccagataaag tatccagatg gcaatagcaa aagtaagcca acgaagcata attactcctg
     1381 tgttgttagt aagaaaaatg ggtttgccgg taagcttcgg cgctgaagtc aggcttccgc
     1441 accgaccggg tttcaggctg cttgttccgg caaccaggtg aattcgatta actcgacgtt
     1501 catgtcttca tcgatggaaa attccagctt cacccggtct ccgaccttga ccaggggcag
     1561 atcgataa
//
LOCUS       DAOQNZ010001101         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62867_length_1567_cov_4.692460, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001101 DAOQNZ010000000
VERSION     DAOQNZ010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62867_length_1567_cov_4.692460"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1142)
                     /locus_tag="PKW73_16755"
     CDS             complement(138..1142)
                     /locus_tag="PKW73_16755"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75004.1"
                     /translation="MNCYRMLLPALSLIVVPVTLPMDESATYYGPGILAPVCSAHARP
                     TLFVDPSPWELKHAFNSLMVLQGTAKGKAFNEAEKYLAQARSAGEDAGYIKMAESSLS
                     ISRRQFTLAEKQALQALSLDPHNSRFLAQMGRVQLMLDREEEAKKYFLAAIHCRNPNS
                     DACLVALSGFESGDCLEEAYEAGKVMIKANPSPAGAYYRTARIARSLGKFEEGKELMV
                     KAIHIDPARKEHWYTLAEMELGLKNWKGALAASDKVLQLSGKNDCREAIFVLPIKAKA
                     YEGLGDYAGAVKQWSLALEKVPDTRPVLVRRAACYAKLGDKSAEKRDLAKVKIVDQGL
                     "
     gene            complement(1188..>1567)
                     /locus_tag="PKW73_16760"
     CDS             complement(1188..>1567)
                     /locus_tag="PKW73_16760"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA75005.1"
                     /translation="DYLPKPFSMKELSARVRALLRRPEPMVSELVQLGPLQIDLKFHK
                     VMRGQEELNLLAKEISLLEFLVRHRGQVFSVDDLLNKVWHSESDSSEDAVRQCVTRLR
                     KKIDGEGENSLIVTVKGLGYKVV"
BASE COUNT          378 a          437 c          369 g          383 t
ORIGIN      
        1 gattttatct gaggcaacta tttcttgcgg gtacgattta ttgtgagtca atgcgaataa
       61 tttcgcttgc gcctaagttt gtgaaaacag agcccggtcg tctcatccgc caaagactgc
      121 atgtccggca actgccgcta gagaccctgg tctactatct tcaccttagc caggtcgcgc
      181 ttctctgccg acttatcgcc cagtttggca tagcaagccg ctcgcctcac aagcaccggt
      241 cgagtatcag gtaccttttc cagagccagg ctccattgct ttacggcacc ggcataatcc
      301 ccgagacctt cataagcttt ggccttgatc ggcaaaacaa aaatagcttc ccgacagtca
      361 tttttgccgc tcaactgtaa caccttgtca ctggctgcaa gagcaccttt ccaattcttc
      421 aatcccaact ccatttcggc caaggtgtac cagtgttcct ttcgagcagg gtcaatatga
      481 atagccttga ccatcagctc ttttccttct tcgaatttgc ctaaactgcg agcgattctg
      541 gcggtgcgat agtaagcgcc ggccggagaa ggattggcct taatcatgac tttcccggcc
      601 tcgtaggcct cctccagaca atcaccgctt tcgaagccgg atagtgccac cagacaggca
      661 tcgctattgg gattgcggca gtgaatcgcc gctagaaagt acttcttggc ttcctcctct
      721 ctgtccagca tcaattgcac gcgccccatc tgggctagaa aacgtgaatt atgcggatcc
      781 agagaaaggg cctgaagggc ttgtttctcg gccagggtaa actgccttcg actgatggag
      841 agggaactct cagccatctt aatatagcca gcatcttcac cggcactccg ggcttgcgca
      901 agatactttt cggcttcgtt gaaggccttt ccttttgcag tgccctgcaa aaccatcaaa
      961 ctattaaagg cgtgtttcag ttcccagggg cttggatcta caaacagagt cggtctagca
     1021 tgagcggaac agacaggtgc cagtatgccg ggtccgtaat aggtagccga ctcatccatc
     1081 ggcagcgtga caggaaccac aattagagag agtgcaggaa ggagcattcg atagcaattc
     1141 ataaagcaag ctccacagat ccaaagactc agatggtagg caattcctca aacaactttg
     1201 tagcccaggc ctttcacggt gacgattagc gaattttcgc cttccccatc aattttctta
     1261 cgcaaacgag taacacactg cctgacggca tcttcggagc tgtccgattc ggaatgccag
     1321 accttgttga gcaggtcatc aacgctaaaa acctgccccc ggtgccgtac aagaaattcc
     1381 agcagggaaa tttccttggc taaaaggttc aattcctctt gcccccgcat cactttatga
     1441 aatttcaggt caatttgcag gggacccagt tgcaccagct ccgagaccat tggctccggg
     1501 cgacgcaaga gtgcccgcac ccgggcggaa agctctttca tgctgaaggg cttgggcaaa
     1561 taatcgt
//
LOCUS       DAOQNZ010001102         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_62901_length_1567_cov_3.034392, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001102 DAOQNZ010000000
VERSION     DAOQNZ010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62901_length_1567_cov_3.034392"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..290)
                     /locus_tag="PKW73_16765"
     tRNA            complement(205..290)
                     /locus_tag="PKW73_16765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Ser,seq:tga)
     gene            369..1205
                     /locus_tag="PKW73_16770"
     CDS             369..1205
                     /locus_tag="PKW73_16770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNA75006.1"
                     /translation="MSQKKASSSRLDQILIEKGLFTSREVARTAIMDGGILVNGEKVT
                     KPGAAVKDSAIIEVLEKFQKPPYVSRGGLKLAKALDWFQVDVNGLIAIDVGASTGGFT
                     DCLLKHGAAFVYAVDVGYGQIDWSLRTDERVKVVERCNIRYLNPESLNLKATGEPTLA
                     VMDVSFISVTKTIPNTINLMKEGEGQLIILIKPQFEAGRGKVAKGGVVKDKQTHIELL
                     QSCLSQLQALNLTVAGLTYSPIKGPSGNIEYLLHLLKGEDSTSADIDIPALVEEAFLA
                     LN"
     gene            complement(1215..>1567)
                     /locus_tag="PKW73_16775"
     CDS             complement(1215..>1567)
                     /locus_tag="PKW73_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNA75007.1"
                     /translation="VKSVSAPQTQLKAGKKITVQAVGTFTIHGKSVVKTVPVDVTYLA
                     PCSQPGVKFPDCGMIQIRAELPVAFKDHDIKRPEIVFQKLADTVFVKISATARQVKEQ
                     EKGSPKAKKEKGAK"
BASE COUNT          407 a          404 c          390 g          366 t
ORIGIN      
        1 aaaacctgcc atttgcagat gagtgcctgt agttcgaaca gttactctgt ttactccaag
       61 gtctttagcc tccccttcag cagtaccgaa ctgcaaaggc gaagctgaac tgagcgttac
      121 ctcaccgtgg tcaaggcttg tcagttggtt gttggatagg gcgacccaga tttgcattgc
      181 tgatttcaaa tagggccgga aagatggcgg agcaggaggg actcgaaccc ccaacccctt
      241 tcagggcagc cgctttcaag gcggtgtctt catccagcca ggtctgctcc acaaaccccc
      301 ggttctgcaa ttatttataa ccgactcttg ctattctaac ccaagaaaga tgccaaagag
      361 taaccaagat gtcacagaaa aaagcaagct cctccagact ggaccagatt ctaattgaaa
      421 aaggactgtt tacctcgaga gaagtagccc gcaccgccat catggatggt ggcattctcg
      481 tgaacggtga gaaagtgaca aaaccaggcg cagccgtaaa ggacagcgcc ataatcgaag
      541 tgctggaaaa atttcagaaa ccgccctatg taagccgtgg cggtctgaag ttggctaaag
      601 ccctggattg gtttcaagtc gacgtgaatg gtttgatcgc catcgacgtg ggtgcatcca
      661 ccggcggctt taccgactgc ctgctgaagc acggagcagc ctttgtctac gccgttgacg
      721 tgggctatgg tcaaatagac tggagcctga gaacggacga gcgggtgaag gtggtggagc
      781 gctgtaacat tcgttatcta aatcccgaga gcctcaatct caaagccaca ggcgaaccga
      841 ctctagcggt catggacgtt tccttcatat ccgtcaccaa aaccattccc aacacaatca
      901 atctcatgaa agagggagag ggacaactga ttatattgat caagccacag tttgaagccg
      961 ggcgaggcaa agttgctaaa ggcggcgtcg tcaaagacaa acaaacacac atcgaattgc
     1021 ttcagtcctg cctgagccag ttgcaagcgc tcaacctgac cgtggccggg cttacatatt
     1081 ctccaatcaa gggaccgtcg ggcaatattg agtatttgct gcacttacta aaaggcgaag
     1141 atagcacaag tgccgatatc gacataccgg cacttgtgga agaagcattt ctcgctttga
     1201 actgaggaaa gggactattt tgcccctttc tctttcttcg ctttagggct gcccttttct
     1261 tgttccttaa cctgtcttgc cgtagcggag attttcacaa ataccgtatc ggccagtttc
     1321 tggaagacta tctcaggccg tttgatgtcg tgatctttaa aagccacagg caattccgct
     1381 ctaatctgaa tcatgccgca gtcagggaat ttgaccccgg gctgactgca aggcgccaga
     1441 taagtgacgt ccacgggcac agtcttcaca acactcttac cgtgaatagt gaaggtgccc
     1501 accgcctgca cggtgatctt cttaccggcc tttaactggg tctgaggagc actgacactc
     1561 ttaactg
//
LOCUS       DAOQNZ010001103         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_63110_length_1564_cov_4.176938, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001103 DAOQNZ010000000
VERSION     DAOQNZ010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63110_length_1564_cov_4.176938"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PKW73_16780"
     CDS             <1..440
                     /locus_tag="PKW73_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75008.1"
                     /translation="APIVGSGNNLRSMAYVDNICQGLMLCERVAGAAGRTYWIADQEP
                     YTMNQIVDTIEKVLSKDFNIACRGSRMRLPDLASEVALMVDAGLQGAGLYHQKFHVLS
                     EMNKNIACSIKRAEQELGYKPTVALEEGMRRSIDWILKRGQKI"
     gene            491..>1564
                     /locus_tag="PKW73_16785"
     CDS             491..>1564
                     /locus_tag="PKW73_16785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA75009.1"
                     /translation="MLIFSCLVVSLAYWLFFSYAFKQGQAIEYEGPCLWGAYRIAHGL
                     NPYPLESLSQSPYAVLIYPPVYFYLASLFLHGEPSYLMPRLISMVASLATAGFYYAFL
                     RSLNLSRLASGSGSCLLMSFMAIWLWSAKGRVDALSIALTCLGLFLYGAACKNNKPGS
                     LILSAAALMLATMTKQPSALFVPALALDLVCRRKFKNLLLFSGSYLLFLVSSILAMQV
                     VTQGGFLAHMRFASHMPFEWRYLFERLALMASDIPKFLLAGFAAVALLLKGKLRLGQE
                     DNLRLALLMLATTIPVTFYTAGTAYSNVNHFLVTLLPLPLLIASALDSGRAKLEENSD
                     PPIFFAFLTGTTLILSSLIIVFLG"
BASE COUNT          322 a          466 c          377 g          399 t
ORIGIN      
        1 aggcgcccat agtgggaagc ggcaacaatc ttcgctccat ggcctacgta gacaatatct
       61 gccagggcct catgctctgc gagcgagtgg ccggcgccgc cggtcgcacc tattggatcg
      121 ccgaccagga gccctatacg atgaatcaaa tcgtagatac cattgagaag gtattgagca
      181 aagacttcaa catcgcttgc cggggcagcc gcatgcgctt acctgatctg gcatcggaag
      241 tggctctcat ggtcgatgcc ggactgcaag gggccgggct gtaccatcaa aaatttcacg
      301 tgctttcgga aatgaataaa aacattgcct gttccataaa gcgggccgag caggagttgg
      361 gctacaaacc cacggtggct ctggaagagg gtatgcgaag gagtatagat tggattctga
      421 agcgcggtca gaaaatctag acggtttaaa aaagccccgc cccggccgaa caaaccttgt
      481 cttcaaggcg atgttgatct tctcttgcct ggtggtgtct cttgcttact ggcttttctt
      541 cagctatgcc ttcaaacaag gtcaagccat agagtacgaa gggccctgcc tctggggtgc
      601 ctatcgcatc gcccacgggc tcaaccccta tccactagag agcctcagcc agagccccta
      661 tgcagtacta atctatccac cggtctactt ctatctcgct tccctgttct tgcacggtga
      721 gccttcctat ttgatgcctc gcctcatttc catggtggca agcctggcca cggcaggctt
      781 ctattatgcc tttttacgca gtctcaacct cagtcgactt gcttccggca gcggcagttg
      841 cctcttgatg agcttcatgg ccatctggct ctggtcggcg aaaggcagag tggacgctct
      901 ctccattgcc cttacctgcc tggggctctt cctctacggt gccgcctgta aaaacaacaa
      961 gcctggaagc ctcatcttga gcgcagccgc cctcatgctt gccaccatga ccaaacaacc
     1021 gtccgccctc tttgtcccgg cactggccct ggatttggtt tgtcggcgga aattcaagaa
     1081 cctgcttctc ttctccggat cttacctgct ctttcttgtg tcaagcatac tggccatgca
     1141 agttgtcacc cagggcggtt ttctggcaca catgcgtttt gcctctcaca tgccctttga
     1201 gtggcgctat ctttttgagc gattggctct catggctagc gatattccaa aatttttgct
     1261 ggcgggattt gcggcagtag cactccttct aaaaggcaaa ctacgcttgg gtcaggaaga
     1321 caacctgaga ctggccttgc tcatgcttgc cactaccatt ccagtgacct tctatacagc
     1381 cggcaccgct tattccaacg tcaaccactt tctggtgacg ctcctgcccc tgcccctcct
     1441 tattgccagc gccctggaca gcggccgcgc aaaactggaa gaaaattccg atccaccaat
     1501 tttctttgct tttcttacgg gcaccaccct catcttgtca agcctgatca ttgtctttct
     1561 tggc
//
LOCUS       DAOQNZ010001104         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_63141_length_1564_cov_2.988072, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001104 DAOQNZ010000000
VERSION     DAOQNZ010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63141_length_1564_cov_2.988072"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /gene="hutI"
                     /locus_tag="PKW73_16790"
     CDS             complement(<1..1053)
                     /gene="hutI"
                     /locus_tag="PKW73_16790"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNA75010.1"
                     /translation="MTERQEHQLKAVINAGELVTAHGDGPKTGEALSDLGIIKGGAVV
                     YRDDTIVAVGPTEEMLSAYKPEQIVDADGRLVMPGFVDPHTHLVHMGSRHEEYECKIA
                     GKSYADLHKVGGIRYTVGMTRAAGEDELYEKARRDLDIMLLYGTTTVEAKSGYGLNQE
                     TELKLLRVAKRLKETHPIELVSTFLGAHTVPEEFKDDKAGYVKLVRQMMAEAAPLAEF
                     CDAWCDSLGFSVEECRDILNEAKKHGLKLKLHVEQTGWSGGGELAAEMQVASADHLDF
                     LSRAGIEQMHQKGVVGVLLPGVTYHLMEFKKEIAVKDMIDGGLAIALATDYNPGTCRT
                     QSMQAILEAACRLYRLT"
     gene            complement(1207..1416)
                     /locus_tag="PKW73_16795"
     CDS             complement(1207..1416)
                     /locus_tag="PKW73_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75011.1"
                     /translation="MASLLAQSSMALYCSSALDTSSPKSTETSVPSMASMATINSRFL
                     SISIFKVLRETYSPTKAMTKEAAAQ"
BASE COUNT          298 a          446 c          413 g          407 t
ORIGIN      
        1 ggtgagcctg taaaggcggc aggccgcttc caatatggct tgcatcgatt gagtgcggca
       61 ggtgccggga ttgtagtcgg tggccagggc aatggccagg ccgccatcga tcatgtcttt
      121 gacggcgatt tcttttttga actccatcaa atgataggtg acgcctggca gaagcacgcc
      181 taccacgccc ttctggtgca tctgttcgat gccagcccgg gagaggaaat ctaagtgatc
      241 ggcggaagcc acttgcatct cggctgccag ttcgccgccg cccgaccaac cggtttgctc
      301 cacatgcaac ttcagcttca gtccatgttt ttttgcctcg ttgagaatat cgcggcattc
      361 ctcgacactg aaaccaagtg agtcgcacca ggcatcgcag aactccgcca ggggagccgc
      421 ttctgccatc atctgccgca ccagtttcac atagccggct ttgtcgtctt tgaattcttc
      481 gggaacagtg tgggcgccga ggaaggttga aaccagctcg atcgggtgag tctctttcag
      541 tcgtttggct acccgcagga gttttagctc agtttcctgg ttcagcccgt agccgctttt
      601 ggcctcgacc gtggtagtgc cgtagagcag catgatatcc agatcgcgcc tggccttttc
      661 gtaaagctcg tcttcaccgg ctgctctggt catgcctacc gtatagcgaa tgccgcctac
      721 tttatgcagg tccgcgtagc ttttgccggc aattttgcat tcatattctt cgtggcgtga
      781 acccatgtgc accaggtgcg tgtgcgggtc cacaaagccg ggcatgacca ggcgtccgtc
      841 ggcatcgaca atctgctccg gtttgtaggc ggagagcatt tcttcagtcg ggcccacggc
      901 gacaattgtg tcatctcgat agacgacggc accgcctttg atgatgccca gatcggataa
      961 agcttcaccg gtcttgggtc cgtccccatg ggcagtgact aattcaccgg cattgataac
     1021 tgctttgagc tgatgctctt gtctctccgt cattaaccgc tctcccaggt tcggaaccat
     1081 tcaaatccct caatttcgcc atattgtatc tgcttcttgt tgccatatag gtgccctgct
     1141 agtattagca ttcgaatttt cttataagtc tcaatatggg tttctcatgt ccctttcacc
     1201 acttatttac tgggcggctg cttccttagt cattgcttta gtggggctgt atgtctcacg
     1261 caagaccttg aaaatagata tggagaggaa tctggagttg attgtggcca tgctggccat
     1321 actcggcaca ctggtttcgg tgcttttagg gctgctcgtg tccagtgccg atgagcaata
     1381 tagagccatg gaagactgcg ccaatagcga agccaccaac atctctcaaa tctttggact
     1441 ctcgcgcggc ttgccccggc ccgggcggcc ctctacggtc gagccatgcc caacgccaag
     1501 cccgtgctcg tggtcgacga cgaccccgag atcttgacga tgctgtcgac ccgcctcgag
     1561 tccc
//
LOCUS       DAOQNZ010001105         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_63203_length_1563_cov_3.648541, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001105 DAOQNZ010000000
VERSION     DAOQNZ010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63203_length_1563_cov_3.648541"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1196
                     /locus_tag="PKW73_16800"
     CDS             69..1196
                     /locus_tag="PKW73_16800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA75012.1"
                     /translation="MSALMPNQPEGKDAGKLASSRLRLMEGQIFAGRYLIKERISRGT
                     LSAVYKAERIGDGLIVALKVLLPKLVVDERAAGLLRHERAANARLTEAGPEDGKDGAP
                     STVQHPSLVCILDSGFDEPSKLPFIVMEYVDGLTLAAKLEKSVRLEERQALNITAKIA
                     MALAHAHARGIIHGDLNPGNIILLPTGAEDCQLKVIDFGIAHIENKDDAKGNLPEVFG
                     SPHYMSPEQCLGQNLDARSDVYSLGCLLYHMVTGFMPYGNNPLSPPLSPQAIIMKQLA
                     NKLPDWRAMQVSETVNTILKGCLQKRPESRYPSMEALLEELNRLSPQLALPQATGLEK
                     AQDRPGKTQRSTTIRVQITLIAVAVSVLLLIIIFLSQAGPR"
     gene            1197..>1563
                     /locus_tag="PKW73_16805"
     CDS             1197..>1563
                     /locus_tag="PKW73_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75013.1"
                     /translation="MKTRKTLLKLTLFTTLAQMQTAALAQGWSIPAGQGPEQTARFEK
                     QSEEQLISQAKLETKRNNAEGALQICNIILKRNPKCAAAYVTKSKNLDFLNKDAEALK
                     CLDEGLKILPQAPGLYREKG"
BASE COUNT          425 a          433 c          407 g          298 t
ORIGIN      
        1 accgtggtgc cgtcggcacc gcgtcccggt ctttgaagca tgaaatatac ggaagcgccg
       61 gcacttaaat gagcgcactc atgcctaatc aaccggaagg caaagatgcc ggcaaattag
      121 ccagctcgag actgcgtctc atggaaggtc agattttcgc cgggcgctat ctgatcaagg
      181 agcgcatttc aaggggcact ctcagcgccg tgtacaaagc cgaaaggata ggggacggtc
      241 tgatcgtagc cctgaaggta ctcttgccca aactggtagt tgacgaaaga gcggccggcc
      301 tgctcaggca tgaaagggca gccaatgcca ggcttaccga agccggcccg gaagacggaa
      361 aggacggcgc accgagtacg gtgcaacatc cgtccctggt ctgtattctg gatagtggtt
      421 tcgatgaacc gtcaaagctg ccgttcatag tcatggagta tgtggatggt ctgaccctgg
      481 cagccaaatt ggaaaagtct gtgagactgg aagagcgaca ggccctcaac attactgcta
      541 agattgccat ggctctagcc catgcccatg caagaggaat catccacgga gacctcaacc
      601 cgggcaatat catacttttg ccgacagggg cggaagactg ccaattgaaa gtgatagatt
      661 tcggcatcgc ccacatcgaa aacaaggatg atgccaaagg caacttaccg gaagtcttcg
      721 gctcaccgca ctacatgagc ccggagcagt gcctgggaca aaacctggat gccagaagcg
      781 acgtctactc actgggctgt cttctttacc atatggtgac cggctttatg ccttacggca
      841 acaacccgct ctctccgccc ttgagcccgc aagcaattat catgaagcaa ctggcaaaca
      901 aactacccga ttggcgcgcc atgcaagttt cagagacagt aaacacaata ctgaaaggct
      961 gcctgcaaaa gcggccggaa agcagatacc catcaatgga ggcgctactg gaagagctaa
     1021 accggctctc cccgcagcta gccttgccac aagcaaccgg actggaaaaa gctcaagacc
     1081 gccccggcaa aacgcagcga agtactacca ttagggtgca aatcaccctg atcgccgtgg
     1141 ctgtttccgt gctcttgcta atcataattt ttctaagcca ggcaggtcct cgctgaatga
     1201 agacaagaaa aacactgctt aaattgacgc tcttcactac cctggcacaa atgcaaacag
     1261 ccgctctggc ccagggttgg tccataccgg ctgggcaagg gccggagcag accgcccgct
     1321 tcgagaaaca gagtgaagag caactaatca gtcaggctaa gctggagact aagcggaaca
     1381 atgccgaagg tgccttgcag atctgcaata tcatcctcaa gcgcaacccc aagtgcgccg
     1441 ccgcttatgt caccaaatct aagaatctgg acttcctcaa caaagacgct gaggctctca
     1501 aatgtcttga cgaaggctta aagattttgc ctcaagcccc cggtctttat cgagagaagg
     1561 ggc
//
LOCUS       DAOQNZ010001106         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_63293_length_1562_cov_3.642336, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001106 DAOQNZ010000000
VERSION     DAOQNZ010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63293_length_1562_cov_3.642336"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PKW73_16810"
     CDS             complement(<1..994)
                     /locus_tag="PKW73_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNA75014.1"
                     /translation="MSLSRKSFLAAFVLPVHFSSDVLKGPEPAVKERRGQTFSCEPCR
                     TFIIVNRWKWNYPQCLSLEVVVMKPVTLEVSCLNEPGASDTVAPAASCAGADGLRVGI
                     SSFAAWMLGVGSIIGSMAWLIHGPMLARAGSLACLTAWFIAGIMALPLALILMELSSM
                     FPSAGGPYVYKFYALKRLIPGSGELLGFLTGWLFWIAIIVGVACMANGLANLLSTMLF
                     GSTTASPFWFSPLVILLLFGATTLLNLQKVDTAARISNLFTFLKLAMALVFALLVLCA
                     PGASLSNVLACRNQSVPSDFWSSVASVLMLAMAGFSFLEMTGCTAAETRDAQRSV"
     gene            997..1464
                     /locus_tag="PKW73_16815"
     CDS             997..1464
                     /locus_tag="PKW73_16815"
                     /inference="COORDINATES: protein motif:HMM:NF025471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HNA75015.1"
                     /translation="MTSKPSETVQRAVDTQPERVRATLRIYHPELNPEQITDLLEISP
                     SVSWRKGDDAYGWTKKPGKKAPSGGWLLSSRQKVKSNNAIAHLDWLMDQLSGSGTVLK
                     QLQDQGFMVDVVVGWHATSWNTTPALTPDIMRRLANQRLPIWFDVYLYDDEDI"
BASE COUNT          434 a          427 c          434 g          267 t
ORIGIN      
        1 gaacgctccg ctgcgcgtcc ctggtttcag cagcggtaca gccggtcatt tccaggaagg
       61 agaagccggc catggccagc atcagcacgg aagcaacgct gctccagaag tcggaaggta
      121 cggactgatt gcggcaggcc agcacattgc tcagggaagc accgggcgca cacagcacca
      181 gcagggcaaa gacaagcgcc atagccaact tgaggaaggt aaagagattg ctgatgcgag
      241 cggctgtatc gaccttttgc aaattcaaaa gggtagtagc accaaacaac aggagaatga
      301 ccagtggtga gaaccagaaa ggtgaagcgg tggtggagcc aaaaagcatg gtggaaagca
      361 gattggcaag cccgttagcc atgcaggcaa cgcccacgat aatggcaatc cagaagagcc
      421 agccggtcaa aaagccgagc aactcaccgc ttcccggaat gagacgcttg agggcgtaga
      481 acttatatac atagggtccg cccgctgatg gaaacatgga agacaattcc atgagaatca
      541 aggctaaagg caaggccata atgccggcga tgaaccaggc cgtcaaacag gccagtgaac
      601 cggctcgcgc cagcatggga ccgtggataa gccaggccat ggaaccaatt atggaaccga
      661 ctccaagcat ccaggcggca aaggagctga tacccactct cagaccatcg gctccggcgc
      721 aagacgctgc gggagcgaca gtgtcagaag caccgggctc attcaaacag gacacctcta
      781 gagtaaccgg tttcatcacc actacctcca ggctcaaaca ctgagggtag ttccatttcc
      841 agcggttgac aatgatgaaa gtacggcaag gctcgcagga gaaggtctgc cctcgtctct
      901 cctttacagc cggctcagga cctttaagga catctgagct aaaatgaacc ggtaggacaa
      961 atgcggccag aaaagacttt ctggagaggg acaaccttga cttccaaacc ttcagagaca
     1021 gttcagagag cggtagacac ccaaccggaa agagtgcgcg ccactctgcg catttatcat
     1081 cccgaactca atcccgagca aatcaccgac ttgctggaga tcagcccttc ggtatcctgg
     1141 cgcaaaggcg atgatgccta cggctggacg aaaaaaccag ggaagaaagc tccatccgga
     1201 ggctggcttc tgagcagtcg gcagaaagtg aaaagcaata atgccatcgc ccacctggat
     1261 tggctcatgg accaactgtc aggctccggt accgtgctca aacaattaca agaccagggc
     1321 ttcatggttg atgtggtggt gggctggcat gccacttcct ggaatacgac gccggctctc
     1381 acacccgaca tcatgcgccg cctggccaat cagcgcctgc ctatctggtt tgacgtttac
     1441 ctctacgacg atgaagatat ttagttggcg gtagccgccg ccatattgca catcggcaat
     1501 tttgcggaag ccgcgcggat cccacaaata ggaccagtcg gcagtggata ccagaagctt
     1561 gc
//
LOCUS       DAOQNZ010001107         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_63389_length_1561_cov_3.363878, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001107 DAOQNZ010000000
VERSION     DAOQNZ010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63389_length_1561_cov_3.363878"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKW73_16820"
     CDS             complement(<1..496)
                     /locus_tag="PKW73_16820"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA75016.1"
                     /translation="MALTDFAVENYRSIRNVWLKLQPVNVIIGPNGSGKSNLYRAIYL
                     VSCAARGQFASALAEEGGIQSVLFAGPRKEPGKRETFKLSVRVGKFEYNLECGTCGDV
                     VKPGFFAGDPEIKKEEVFIVENGERHGLLERRRRSIWARNAAGKQVDYTVQVPDNESI
                     LTGLN"
     gene            complement(508..>1561)
                     /locus_tag="PKW73_16825"
     CDS             complement(508..>1561)
                     /locus_tag="PKW73_16825"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA75017.1"
                     /translation="YRSLYLLWSAAAGNLAGAIADEGGMPSILSAGSLRKPGQKSVTL
                     SVDIDDLSYQIVIGLVPLSDRIGDELMGYFQKDPDIKIEKVNVDYRGKQISLLARKRG
                     YISARNMDGRPIEYPLVVAHCESVLTGLREPHLFPDLSKLRSEILSWRFYHNFRTDFH
                     SPLRRPQVPVFTPVLSHDGGDLASALATIAAVEGAAAINEAVSDAFPDSTIGFKADDD
                     VLALTMKMPGLNRSFSALELSDGTLQYLCLIAALLSPRPASFLALNEPETSIHPSLYE
                     AMARLIVQAGKKSQLWVTTHSEELARYIEKAAGVKAIELEKVNGATCVVGAKLSPESE
                     EEDYDDDDRSDSSRAG"
BASE COUNT          399 a          432 c          380 g          350 t
ORIGIN      
        1 cattcaagcc cgttaaaatt gactcgttat caggcacctg caccgtgtag tcgacctgct
       61 tgccggcggc gttgcgcgcc caaatagagc gccgacgacg ctccagcaag ccatgtcgtt
      121 cgccgttttc tacgatgaag acttcctctt tctttatctc aggatcaccg gcgaagaatc
      181 ccggtttgac gacgtctccg caagtaccgc attccaggtt gtactcaaac ttgccgacgc
      241 gcactgaaag tttaaaggtc tcgcgcttac caggttcttt gcgaggtccg gcaaagagca
      301 ccgactggat accgccttct tcagccaggg cagaggcaaa ctgcccccgt gctgcgcagg
      361 aaacaagata gatagcccgg taaagattac ttttgccgct accatttgga ccaatgatta
      421 cattcacagg ctgcaatttg agccagacat tccttatgga acgatagttc tcgacagcga
      481 aatccgtcag cgccattact gcctccctta gcctgctctt gaggaatcgg accggtcatc
      541 gtcatcatag tcttcctcct cactttccgg agagagcttg gcacccacca cacaagtggc
      601 accatttacc ttttcaagtt caatagcttt gacgccggca gccttctcga tatagcgagc
      661 taattcctca ctatgggtag ttacccaaag ctgtgatttc ttgccggctt gtacgataag
      721 gcgggccata gcctcgtaca agcttggatg aatgctggtt tccggttcgt tcaaggccag
      781 aaatgatgcc ggacgaggcg acagcaaagc ggcaatcaaa caaagatact gcaaggtacc
      841 atccgataat tccagagccg agaaggaacg attcaacccg ggcattttca tagtaagcgc
      901 aagaacatca tcgtcggctt tgaaaccaat ggtggaatcg gggaaggcat cgctgacggc
      961 ttcattgatg gcagccgcac cttcgacggc ggcaatggtg gctagcgctg aagccagatc
     1021 gccaccatca tgactgagta cgggagtgaa gaccggcacc tgcggtcggc gcaggggcga
     1081 atgaaaatcc gtgcggaaat tatgatagaa acgccaactc aatatctcgc ttcgcaactt
     1141 ggatagatcg ggaaacaagt gcggctcccg caaccccgtc agcaccgact cacaatgagc
     1201 caccaccagt ggatattcga ttggtcgtcc atccatgttg cgggcggaga tatagcctct
     1261 ctttcttgcc agtagcgaaa tctgcttgcc gcgataatcg acatttacct tttcaatttt
     1321 tatatcagga tctttctgaa agtaacccat cagctcatcg ccaatgcgat cactcaaagg
     1381 caccagacca ataacaattt gatagctcag atcatcgata tctacagaga gcgtcacgct
     1441 cttttgtccg ggtttacgca aactgccggc cgacaagatg gaaggcatac cgccttcatc
     1501 agcgatggca ccggcaaggt taccggcagc agccgaccag agcagataaa gagagcgata
     1561 a
//
LOCUS       DAOQNZ010001108         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_63416_length_1561_cov_2.875830, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001108 DAOQNZ010000000
VERSION     DAOQNZ010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63416_length_1561_cov_2.875830"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKW73_16830"
     CDS             complement(<1..240)
                     /locus_tag="PKW73_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75018.1"
                     /translation="MKRQLTVLVLLTVICSGYCASAARAKGANDAASASVPATLLVQK
                     LQNLGRTKWFFAKDFVRVDLPIASMLYDSRTSKATF"
     gene            complement(303..>1561)
                     /locus_tag="PKW73_16835"
     CDS             complement(303..>1561)
                     /locus_tag="PKW73_16835"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA75019.1"
                     /translation="VRPELTAVPGARLSFSRASGLTGKPLRLVLTSPDSELLDKTAET
                     IKDQMRGIDGLYDVTSSAALLRPEILVTPDFDRSARQGVSVQSIARTALIATLGDAEA
                     NLAKFNLEDRQVNIRVQLAPAYRKDLRVIENLKVLTSSGKLVPLSSVAHVDFGCGPFQ
                     IDRFDRQRQVTLEAGLESSITLGQALKQVHNLPGFKEMPASIVELPLGDAEIQRDIFS
                     GFGTAIGAAVLLIYAVLVVLFGGFLYPLTIMVSLPLSLCGALMGLVLWHQSIGLYALI
                     GITMLMGLVTKNAILLVEYALLKMKEGLPQRTAIMMAGETRMQPILMTTIAMIAGMMP
                     IAMGIGAGSEARSPMAISVIGGLITSTLFTLVVVPVVFTYVDDFQNWLKKLVRGRAGE
                     PEAKANLDARRRSFESSESTVRGDKF"
BASE COUNT          415 a          433 c          403 g          310 t
ORIGIN      
        1 gaaagtagct ttgctcgtgc ggctgtcgta aagcatagag gctatcggca aatccactct
       61 tacaaagtcc ttggcaaaaa accacttggt tcgccccagg ttttgcagct tttgcaccag
      121 tagcgtggcc ggaacactgg cggaggcggc gtcgttagcg cccttagccc gggcggctga
      181 ggcgcaatag ccagagcaaa tcacagttaa caagaccaga acggtcaatt gccttttcaa
      241 agtttctcca tcccggctga gctaatggtc aaaaatgcag gctctatttt agcgccggtc
      301 aactagaatt tgtcgccccg caccgtagat tccgaagact cgaaggaccg cctgcgggca
      361 tccaaattcg cctttgcctc cggctcaccg gctctacctc gcaccagttt cttgagccaa
      421 ttttggaaat catcgacata ggtaaagacc accggcacca caaccagggt aaacaaagta
      481 gatgtaatga gtccgccaat aacggaaata gccatgggtg agcgggcttc cgaaccggcg
      541 ccgatgccca tggcgatagg catcatgccg gcgatcatgg caatagttgt catcaagata
      601 ggctgcatgc gggtctcccc ggccatcatg atggcggtgc gttgcggcag accttccttc
      661 attttcaaaa gggcgtattc caccagcaaa atagcgttct tggtgaccag acccataagc
      721 atggtgatac caatcaatgc gtagaggccg attgattgat gccagagcac cagacccatg
      781 agagcaccgc ataaactcaa aggcagagat accataattg tcagaggata aaggaaacca
      841 ccgaagagaa ccaccagcac tgcatagatg agcaggactg ccgcaccgat ggcggtgccg
      901 aagccgctaa aaatatctct ttgaatttca gcatcaccga ggggcaattc cacaatggat
      961 gccggcatct ctttgaagcc tggcaagttg tgcacctgct tgagagcctg accaagagta
     1021 atactggatt ccagaccggc ttccagagtc acctgcctct gccggtcgaa acggtcaatt
     1081 tgaaagggac cgcagccgaa gtccacatga gcaacggaag aaagcggcac cagtttcccg
     1141 gatgaagtga gcaccttcaa attttcaatc acccgcaaat ctttgcgata agccggcgcc
     1201 aactgcaccc tgatattgac ctggcgatcc tcgagattga acttggccag attggcttcc
     1261 gcatcaccaa gagtagcaat gagggccgtc ctggcaatag actgcaccga aactccctgg
     1321 cgggcggaac ggtcaaaatc aggcgtaacc agaatctccg gccgcaatag cgctgccgaa
     1381 gaagtaacat cataaagccc gtcaataccg cgcatttgat ctttgatagt ttccgccgtc
     1441 ttatccagca attccgaatc aggcgaagtt aagaccaggc gcaggggttt gccggtcaga
     1501 ccggaggccc gcgagaaact gagcctggca ccgggaacag cggtcaactc aggtcgcacc
     1561 g
//
LOCUS       DAOQNZ010001109         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_63652_length_1558_cov_3.821025, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001109 DAOQNZ010000000
VERSION     DAOQNZ010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63652_length_1558_cov_3.821025"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKW73_16840"
     CDS             complement(<1..593)
                     /locus_tag="PKW73_16840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA75020.1"
                     /translation="MPNSPNFLTTAGLAFLYLLAPMAISASSPALAQPQKSTLGADTA
                     GATSKSTPATKKVFSLGTSKIRNISKFHATEKIDLGNFNLFHGNTSQAIKAYRLALAI
                     DPNRFEAHQGLCNCYMRQGKVAMATNEALAMNQLRPKDLNTLILLGNLYKNQGKLAEA
                     IRYLEQAERQGAKSDALHTALGLALAQSNGLDEASRHL"
     gene            747..1163
                     /locus_tag="PKW73_16845"
     CDS             747..1163
                     /locus_tag="PKW73_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75021.1"
                     /translation="MKDSPEIVGIFLCERILQDVFRPDSVTLVNVHNSIGSHAFPTLV
                     PVLYAFAQLRGFKKVFSYKFNIIDPKGTVIAASAPGNVEPGADPTFLLKVVSAFPALV
                     FEGAGNYAITLEIDGSVMGEVPFKVEQLAQPEPAAV"
     gene            complement(1197..>1558)
                     /locus_tag="PKW73_16850"
     CDS             complement(1197..>1558)
                     /locus_tag="PKW73_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75022.1"
                     /translation="IDWFKQNDMGSITVKKQSDVKVSDFKPGDIVIPEKVDGSQHAMV
                     VSRVPENWGYPSGALMFNGNTGLNLPGGPGKPHFRAAQEVIGQGTDIYNHHHGQFNSR
                     MHTNPYLGGKVRILRFN"
BASE COUNT          336 a          368 c          436 g          418 t
ORIGIN      
        1 aggtggcgcg aggcctcatc gagcccatta ctctgggcca gtgccagccc cagagccgta
       61 tgcagggcat ccgacttcgc cccctggcgc tcggcttgct ccaggtatct aattgcctct
      121 gccagtttgc cctggttctt gtagagattg cctaaaagaa tcagcgtgtt caggtctttt
      181 ggcctgagct gattcatagc cagcgcttca ttggtagcca tggctacttt gccctgtctc
      241 atatagcagt tgcatagccc ctggtgtgct tcaaagcgat tagggtcaat agccagtgcc
      301 aatctgtagg cttttatagc ttgactggta ttgccgtgaa aaagattgaa attgccgagg
      361 tcaattttct ctgtggcatg aaacttagat atgttgcgaa tcttgcttgt gccaagggag
      421 aagactttct tggttgcagg ggtagattta ctggtggcac cggcagtatc ggcgccgaga
      481 gtacttttct gcggttgggc gagcgccggg ctcgaagcgc tgatggccat cggcgccaga
      541 aggtaaagaa aggcaaggcc tgcagtagta aggaaatttg gagagtttgg cataagttac
      601 tgacaattat gtcagaagag ggtgggggcg gcaaaaccgc agaagcattt gtttcgttag
      661 gggaagccaa gataagtcgg gcggccgcgg cttgttcgcc atttgctatt atctgacggt
      721 gagtcggaca cggaagcgag agataaatga aagacagccc tgaaattgta ggaattttcc
      781 tctgcgagcg tatcttgcaa gatgttttca gaccggattc agtcaccctg gtgaatgtgc
      841 ataattccat cggatcacat gcctttccta ctctggtgcc ggtgctttat gcctttgctc
      901 agttgagagg cttcaagaag gtattctctt acaagttcaa catcatcgat cctaaaggta
      961 cggtgatagc tgcctctgct cccggtaatg tagagcctgg tgccgatcct actttccttt
     1021 tgaaggtagt cagtgctttt cctgccctcg tatttgaagg tgctggaaac tacgcgatca
     1081 cgctggagat agacggcagt gtcatgggtg aggtgccgtt caaggtggaa cagttggctc
     1141 agccggagcc ggccgcggtc tagtttttct gtcggcaacc ggtaacaaga ccggtctcag
     1201 ttaaatctga gaatcctaac cttgccgccc aggtaggggt tggtatgcat gcggctattg
     1261 aactgaccgt gatgatgatt gtagatgtca gttccctgcc ctattacctc ctgagcggct
     1321 ctgaaatgtg gcttgcccgg accgcccggt agattcaagc cggtattgcc gttgaacatg
     1381 agggcgcctg aagggtaacc ccagttttct ggaacccttg aaaccaccat agcgtgctgg
     1441 ctgccgtcaa ccttctcggg aatgacgata tctccaggtt tgaagtcact gactttcacg
     1501 tcgctctgtt tctttactgt gatggagccc atgtcgtttt gcttgaacca gtcaatga
//
LOCUS       DAOQNZ010001110         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_63913_length_1555_cov_3.736000, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001110 DAOQNZ010000000
VERSION     DAOQNZ010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63913_length_1555_cov_3.736000"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PKW73_16855"
     CDS             <1..712
                     /locus_tag="PKW73_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75023.1"
                     /translation="PVLAAGVAIPLAGAADNSKIKAVRLIQRHHYFGDTETVISLNGV
                     RINNLGGMRFKLAAKAPDWRFCIYRDDDKTIFLNSFQWLKQNGIVNNFLVKERDEYYP
                     KKLPIRESNFSGFKIKWIKSGEETCEYLPLANIAPPQAESILYAVYKMPTNGGIPVRL
                     NKPAPTVDWLSGSDKGRHHRDLFNTSKIEATLVDKDYFDTPLNFHPVKSVLEVLTSKA
                     KRDASGALDELFESGRKH"
     gene            775..1245
                     /locus_tag="PKW73_16860"
     CDS             775..1245
                     /locus_tag="PKW73_16860"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNA75024.1"
                     /translation="MKNDYCNYVLDQLSALPGLHARAMFGGYGIYREGTIFAIIVDDT
                     LFFKVGPLNLSKYEKAGSSPFTYEAKGKSKSVSMSYWLVPAEILEDAGKMTEWAEDAY
                     RVSLAGKKTKPTTKTSRPESKTAKPSTKASKPESKTTKPSTKTSKPRAKSRQSG"
     gene            1352..>1555
                     /locus_tag="PKW73_16865"
     CDS             1352..>1555
                     /locus_tag="PKW73_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75025.1"
                     /translation="MPTSTLQDQAGTANSVASEAEAVLPSVRRGPLTMGLVWVSMVAS
                     FPGVLIGLSWHKQGFPLPEVLSSL"
BASE COUNT          421 a          420 c          379 g          335 t
ORIGIN      
        1 gccagtgctt gccgccggtg tcgccatccc tttggcggga gcagccgata attcaaagat
       61 aaaggcagtc aggctgatac agcgccatca ctattttggg gacacagaaa cagtcatttc
      121 cttaaatggt gttcgcatca ataatctggg gggcatgcgc tttaaactgg cagcaaaagc
      181 cccggactgg cgcttttgca tttaccggga tgatgacaag actatatttc tcaattcctt
      241 ccagtggctc aaacaaaatg gaatagtcaa caacttcctg gttaaggaga gagacgagta
      301 ttatccgaag aagcttccga tacgagagtc aaatttcagc ggcttcaaaa taaaatggat
      361 taagtccggg gaagagacct gtgaatacct gcctctggca aacattgcgc caccgcaggc
      421 agagtcaata ctttatgcgg tatacaagat gccgacaaat ggaggtatcc cggtgcgcct
      481 caacaagccg gcacccaccg tcgattggtt aagcggcagc gacaaaggcc gccaccaccg
      541 agatttattc aacaccagta aaatcgaagc aaccctggta gacaaagatt acttcgatac
      601 tcctttgaat ttccatccgg tcaaatcagt acttgaggtt cttacaagca aggctaaacg
      661 tgatgcatca ggcgctctcg acgaactctt cgaatcaggc agaaagcatt agcaagcgtc
      721 aggacttctc tgttctgaag tctgaatact caatctctac aaccaggcaa gccgatgaaa
      781 aatgactact gcaactatgt tcttgaccaa ttaagtgcgc ttccgggtct gcatgccagg
      841 gccatgttcg gcggctacgg catctaccgt gagggcacaa tttttgccat catcgtagat
      901 gacacgctct tcttcaaagt aggtccgctc aatctttcca agtacgaaaa ggccggttcc
      961 agccccttca catacgaggc aaaaggcaaa tcaaaatccg tctccatgtc ctactggttg
     1021 gtacccgcag aaatactgga agacgccggc aagatgacgg agtgggccga ggatgcctac
     1081 cgcgtctccc tggcaggcaa gaagacgaag cccacgacta aaacttccag gccagagagc
     1141 aaaaccgcga agccctcgac taaagcatcc aagccagaga gcaaaaccac gaagccctcg
     1201 actaaaactt ccaagcccag ggctaagtcc cgccagtccg ggtagacaga tagattgaga
     1261 gagcgagtta gtagttctcc cattggcccg accctgccgg cgtggtcaaa tactgcctgg
     1321 tttgagagac cccaaccttc ggcagtgcta aatgcctaca tccactcttc aagaccaggc
     1381 cggaacggct aactcagttg cttctgaagc ggaagcggtc ctgccgtcgg tcagaagggg
     1441 ccctctcacc atgggtttgg tctgggtgag catggtggct tcctttcccg gcgttctaat
     1501 cggactgagc tggcataaac aaggctttcc cttaccggaa gtgctctcct ccctt
//
LOCUS       DAOQNZ010001111         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_64779_length_1546_cov_2.616365, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001111 DAOQNZ010000000
VERSION     DAOQNZ010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64779_length_1546_cov_2.616365"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..949
                     /locus_tag="PKW73_16870"
     CDS             266..949
                     /locus_tag="PKW73_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75026.1"
                     /translation="MPELPRTGLKTAGILLALCLTLQAPEALSQVKHDKPAGTTGAQK
                     SSKAGEKAPEKTPDKPSEKAADDKKKAEEKKDNKAEAKPAPEPIIENVVNVQAEDLVR
                     TPSEYLGKNIRFSAQFWNFSSVALDYKPAMFSSKDYFSFLVLRPNSHVPLSELKLAMK
                     IPKDEKDPNGKLLQSLKDKDEVEVTGKVTSVALDEPWVQVLKVKRLKAAPEEKKDEKS
                     DKDKSGKDN"
     gene            971..1348
                     /locus_tag="PKW73_16875"
     CDS             971..1348
                     /locus_tag="PKW73_16875"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNA75027.1"
                     /translation="MVTPESAANVKNLLTALNGKHGILGCILVARDGSVVSTSLPADV
                     DIATIGALSATLFSNNDVSIQRMNRGNLIQMTLLTDQGILHFFEAANHYLVVLTAKGQ
                     RINLEGLIRSVEEQGKSLESMLA"
BASE COUNT          437 a          420 c          378 g          311 t
ORIGIN      
        1 gggatttgac atcccagtat tgccgcatca tgggagagag tttgctttgc tcatcagccg
       61 cttccacttg agcctctttg ccgccttctg ttttccccct gccaacacct cgaacaaaaa
      121 cgccatctga ttctacagag tcgcaaaaga ccccgggaga tttctactta ttgaagcctc
      181 aatattacct gcaccgaagg gctaagttat taagtctctg gtaaactgac taaacggata
      241 aaccattatc agaggaagtc tacctttgcc agaactgcca agaaccggac taaaaactgc
      301 gggcatcctg cttgccctct gcctcacctt gcaggcccct gaggccctga gccaggtcaa
      361 gcacgacaaa ccggccggta caaccggggc gcagaagtct tccaaggccg gagagaaggc
      421 tcccgagaaa actcctgaca agccaagcga gaaagctgcc gacgataaga agaaagcgga
      481 agagaagaaa gacaacaagg cagaagccaa gcctgcaccc gagccgatta ttgaaaacgt
      541 ggtaaacgtt caggccgaag atctggtaag aaccccgagc gaatacctgg gcaagaacat
      601 acgcttttcc gcccagttct ggaatttcag cagcgtagcc cttgactaca agccggccat
      661 gttctcatct aaagactact tttccttcct ggtgctgcgc cccaactccc atgtgcctct
      721 gtcggagttg aaactggcca tgaaaatccc caaagatgag aaggatccaa acggcaaatt
      781 actgcagagc ctcaaagaca aagatgaggt agaagtgacc ggcaaggtta cttctgtggc
      841 cctcgacgaa ccatgggtac aggtgctcaa ggttaaacgc ctgaaagccg cacccgaaga
      901 gaagaaggac gaaaagtccg acaaagacaa atccggcaaa gacaactaaa gaggtagagg
      961 aaaagcactg atggttaccc cagaatcagc ggccaatgtc aagaatttgc ttactgcctt
     1021 gaatggaaaa cacggcatcc tgggctgcat tcttgtagcg agagatggtt cggtagtctc
     1081 cacctccttg ccggccgacg tggacatcgc cactatcggc gcgctctccg ccactctctt
     1141 ttccaacaac gacgtatcca ttcagcgcat gaaccgcggc aacctcattc aaatgaccct
     1201 gctcaccgac cagggcatcc tgcacttttt cgaggccgcc aatcattacc tcgtcgtact
     1261 caccgctaaa gggcaacgca tcaacctgga aggtctgatt agatctgttg aagaacaggg
     1321 caaatccctg gaaagcatgc ttgcataaac aaatatatgc tcgcattatg caagtaatcc
     1381 tcgcataata aagatatgca gatggccgca gagaagcggc aggggaggac tgtgaaggag
     1441 cagcctttag aggtgaagtt agaaaagagg cggactgcct gtggcagacg tcgcgcgttc
     1501 ttgcttgtgg gcacagccct gtccttgagc cttaacttat tgagcc
//
LOCUS       DAOQNZ010001112         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_64844_length_1545_cov_3.153020, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001112 DAOQNZ010000000
VERSION     DAOQNZ010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64844_length_1545_cov_3.153020"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKW73_16880"
     CDS             complement(<1..341)
                     /locus_tag="PKW73_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNA75028.1"
                     /translation="MTAPYHGNIPDTTTDKSGNKHGAISMQNSTWLTAGLTDRGLKRK
                     DNQDNFLISDDKRLLVVADGMGGASGGEKASKLAVGTIEAYWNEAPPDYTQPNQVEHW
                     LKEAVARANAAV"
     gene            522..1301
                     /locus_tag="PKW73_16885"
     CDS             522..1301
                     /locus_tag="PKW73_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75029.1"
                     /translation="MGYTPHLRQFHKSRLKFRWLCSCLLLGLPLTITFCGSEAEAGGG
                     RHGVVNFKPVGPLSPRMKDGAALIHHMSRHAGALNDYSLIFETITFKEKETVSEKGRL
                     YFKKPKLMRLEEIGEYKNGSVAVIGKDGKARAHAGGLVKFITLTMNADDKQLNAANGD
                     RMLDSDFASLAGLLERRLQEGNLARATETPAAYEGVDGQTYVLELYRQDEPEKVLKRI
                     YVDPISYLPVRWDDYDYKTPCSSTWSAVKANPGLKDELFDL"
     gene            1347..>1545
                     /locus_tag="PKW73_16890"
     CDS             1347..>1545
                     /locus_tag="PKW73_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75030.1"
                     /translation="MNASVVPKFEPGQSGVGSEPPSHIKLWPRLAPLTRRASVLGALV
                     LLLSAGGNAWAGETQTRKAVKF"
BASE COUNT          317 a          377 c          441 g          410 t
ORIGIN      
        1 acggctgcgt tggctctggc tactgcttcc tttaaccagt gttccacctg gtttggctgc
       61 gtgtagtccg gcggggcttc attccagtat gcttcgatgg tacccacggc tagcttgctg
      121 gctttctcgc cgccgctggc gccgcccatg ccgtcggcga ctaccaggag gcgtttgtcg
      181 tcgcttatga ggaaattgtc ctgattgtct ttacgcttca gaccgcggtc ggtaagccca
      241 gctgtcagcc aggtggagtt ctgcatagaa attgctccgt gtttgttgcc ggacttgtcg
      301 gtagtcgtat ccggtatgtt tccatgatag ggggcggtca taatgatgtt aattcccgaa
      361 aacgggtaaa ttcatgcaat attttctatg cttgttgttc agttcagaat tgggcgcttt
      421 tgtcattttc tacccctgaa ttatcttatt ttgctccggt cggcaatctt ctacaataca
      481 agctgttttt tccgtcccat tgatcggaca aacggaccaa gatgggttat acaccgcacc
      541 ttcgccagtt tcataagagc agactgaagt ttcgctggct ttgttcctgt ttactactgg
      601 gcttgcccct gaccatcacc ttttgcgggt ctgaggccga ggccgggggt gggcgtcatg
      661 gggtggtcaa ctttaaaccg gtgggacccc tttctcccag gatgaaggac ggtgcggctt
      721 tgattcatca tatgagccgc cacgccggtg ccctcaacga ctattctttg atttttgaga
      781 ctattacttt taaggaaaag gaaacagtaa gcgagaaggg ccgtctttat ttcaagaaac
      841 caaaactgat gaggcttgag gagattggag agtacaaaaa tggttctgtg gccgtgatcg
      901 gcaaagacgg caaggctaga gcccatgcgg gaggactggt taagtttatt acccttacca
      961 tgaatgccga tgataagcag ttgaatgccg ccaatggcga ccgtatgctc gactctgatt
     1021 tcgcctccct ggccggtctt ctggagcggc gcttgcagga gggcaatctg gcccgggcta
     1081 cggagacacc ggcggcctac gagggggtgg acggtcagac ttatgtgctc gagctctata
     1141 gacaggatga gccggagaaa gtgttgaagc gaatttatgt ggaccccatc tcatacctgc
     1201 cggtgcgctg ggacgattat gactataaga ccccctgcag tagcacctgg agtgcggtga
     1261 aggcgaaccc ggggctcaag gacgagcttt tcgatttgta gttggcgaat tttcgcctga
     1321 gaggctgata aattaaggag tattcgatga atgcaagcgt cgtacccaag ttcgagcccg
     1381 gacagtctgg agtcggcagt gagcctccat ctcatataaa gttatggccg cggcttgcac
     1441 cgcttacccg tcgtgcctca gttttgggag ccctggtgct tttgctttct gccggcggta
     1501 atgcctgggc gggagagacc cagaccagga aagccgttaa gttct
//
LOCUS       DAOQNZ010001113         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_64903_length_1544_cov_4.855608, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001113 DAOQNZ010000000
VERSION     DAOQNZ010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64903_length_1544_cov_4.855608"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKW73_16895"
     CDS             <1..493
                     /locus_tag="PKW73_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75031.1"
                     /translation="QVAFKSQTGHVLFLALVVLGGAEQFNLVQDFLTQTAKIQGRQGS
                     YLLRGVFIVQSEPSELEKTRQYLVKATNGEDPVARYDSYLPSPFFVHANLLSFAVGAE
                     MSVLPEPVPALETDAGEQENEDQAVEPQVEESGRAVTGFSLVYPSLSRLESSRKPLLA
                     SGG"
     gene            complement(511..>1544)
                     /locus_tag="PKW73_16900"
     CDS             complement(511..>1544)
                     /locus_tag="PKW73_16900"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNA75032.1"
                     /translation="VYSPAVTDFTIMVKDKSYMFVTGPEIVRTVTGEDVTLEELGGAL
                     THNKKSGVAQLLAEDEEDSFYLTRRLLSFIPSNNLDSPPQEAPESVQEPSGQEDLDTI
                     VPVEATKPYDMHKVIEKIVDGGDFFEISPHFAGNIITGFARLAGHSVGIVANQPRVLA
                     GCLDINASVKAARFVRFLDAFNIPVVTFVDVPGYLPGTNQELGGIIRHGAKLLYAYCE
                     ATVPKLTVITRKAYGGAFDVMGSKNVGGDFNFAWPTAEIAVVGAEAAVNLLYRKELKA
                     DQDGKRFKELVQEFKDRFANPYIASQEGYIDDVIKPSETREKLIQALSVQKNKRVERP
                     RRKHGNIPL"
BASE COUNT          308 a          410 c          441 g          385 t
ORIGIN      
        1 gcaggttgcc tttaagtcgc agaccggaca tgtgctgttt ctggctttag tggtgctcgg
       61 tggtgccgag caatttaacc tggtccagga ttttttgacc cagaccgcca agattcaggg
      121 aaggcagggg tcctatcttt tacggggcgt ctttatcgtt caaagcgagc cgtccgaact
      181 ggagaaaact cgccaatacc tggtcaaggc aaccaatggc gaagacccgg ttgctcgcta
      241 tgacagttac ctgccctcgc cctttttcgt gcacgccaat ttgctctctt ttgccgtcgg
      301 cgctgagatg tccgttcttc ccgagcctgt gccggccctt gagaccgatg ccggcgagca
      361 agaaaatgag gaccaggccg ttgagcccca agttgaggaa agcggccggg ccgtgaccgg
      421 tttcagtctg gtttatccca gcctcagtcg tttggagtcg tctcgtaagc ctcttctggc
      481 ctctggaggc tagaaccgac ctgccttgaa ctagagcgga atattgccgt gtttgcggcg
      541 aggtctttcc actcgcttgt tcttttgcac gctgagagcc tggatgagtt tttccctggt
      601 ttccgagggc ttgatcacat catctatata gccttcctgg gaggctatgt aggggttggc
      661 gaagcggtcc ttgaattcct gcaccagttc tttgaagcgc ttgccgtcct ggtctgcctt
      721 caattcctta cggtaaagca agttgacggc cgcctccgcc ccgaccacag caatctcagc
      781 tgtgggccag gcaaaattga aatcgccgcc gacattcttg gagcccatga catcaaaggc
      841 gccaccatag gctttgcgtg tgatgaccgt cagtttggga acggtggctt cgcaataggc
      901 ataaagcagt ttggcgccat gtctgataat gccgcccagt tcctggttcg tgcccggcaa
      961 gtagccgggc acatcgacaa aggtcaccac cggaatattg aaagcatcaa gaaaacgcac
     1021 gaagcgagcg gctttgaccg aagcgttgat atcgaggcaa ccggccagga cgcggggctg
     1081 gttggccact ataccgactg aatgtccggc cagtctggca aagcccgtaa tgatgttgcc
     1141 tgcgaaatga ggtgagatct cgaagaagtc tccgccgtct acgatttttt cgattacctt
     1201 gtgcatgtca taaggcttgg tggcttccac aggcacaata gtatcgaggt cttcctgccc
     1261 tgagggttct tgcacggatt ccggcgcttc ctggggcggc gagtcgaggt tgttggaggg
     1321 aatgaaggac aaaagtcttc ttgtcagata gaaggaatct tcttcgtcct ccgccaggag
     1381 ttgtgctact ccggacttct tgttatgagt gagggcgccc cccagttctt ccagggtgac
     1441 gtcttctccc gtcacggttc gaacgatttc cgggccggtg acgaacatat agcttttgtc
     1501 tttgaccatg atggtgaagt cggtcacggc cgggctatag acgg
//
LOCUS       DAOQNZ010001114         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65281_length_1540_cov_4.350842, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001114 DAOQNZ010000000
VERSION     DAOQNZ010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65281_length_1540_cov_4.350842"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKW73_16905"
     CDS             complement(<1..361)
                     /locus_tag="PKW73_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75033.1"
                     /translation="MLQKSFLVQMTRIGCLGSVVLAPAFTATMAMAQADPGVDPGQQS
                     SAVTPAPDSTPTATPTTGSDAALAGAVSTSDVSALASVTAATQTVDPGQAHAALNNLA
                     GQALVDMSGQLNLMSTLD"
     gene            complement(485..>1540)
                     /locus_tag="PKW73_16910"
     CDS             complement(485..>1540)
                     /locus_tag="PKW73_16910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA75034.1"
                     /translation="AKSKAVQNELQEARSRLAAARATLLEAKALLAADSDTYRRTKEA
                     SLVPGVVAPNEVIVLEQKVESDKEKVNAWQENVNAAENAVTSLIDALDAARKNSENYK
                     DIADYLNVRAPFDGYVTERNMHVGSFVGPLGKSAYPPIVRVQELGLLRIVTPVPEANA
                     GGILPGAPVEFTVSTHPGTKFVGTVARLGNALEQKTRTMPVELNYLNPGWKVLPGMFC
                     RVLWPTRRPNPSLFVPPGAVETKSTLSTFVCRIKDNRVEWVKVERGEMMQGLTEVFGN
                     LQEGDLVAQDCTDALKPGVSVKPLLVESEVKPTAKRPVYDSHDTIYTTPDAERIEMKK
                     TRVNPGQEIQDSEKHDQ"
BASE COUNT          297 a          417 c          446 g          380 t
ORIGIN      
        1 tatccagggt ggacatcagg ttcaactgcc ccgacatgtc cacaagcgcc tgcccggcta
       61 agttgttgag ggcggcatgg gcctgacccg ggtcaacggt ctgggtggcg gcggtgacgg
      121 aagccagtgc tgagacatcg cttgtggaga cggctcccgc cagggcggcg tcgctgccgg
      181 tggtcggtgt tgcagtcgga gttgaatccg gagccggggt aacggcgctg ctttgctgcc
      241 cggggtccac tccggggtcg gcctgggcca tggccatggt ggcggtaaag gccggcgcta
      301 aaaccacgga gcccagacaa ccgatgcggg tcatctggac caggaaagat ttctgcagca
      361 tgaacttatc cttaggtctc gaataggctg aaggctctct cagaagccag tgcctaaagg
      421 gttacttttg cgaagtgagt accattattc caggtttccg ggaggaagga tactaaaagc
      481 ttacttattg atcatgtttt tccgagtcct gaatttcctg acccgggttg accctggtct
      541 tcttcatttc gatgcgctcg gcgtctgggg tggtgtaaat agtgtcgtga gaatcataaa
      601 ctggtcgctt tgcagtcggt ttcacttccg actccaccag caggggctta acgctgaccc
      661 caggcttgag ggcatcggtg cagtcctggg ccaccagatc gccttcctgt aaattgccga
      721 agacctcggt taaaccctgc atcatctctc ccctttcaac tttcacccat tccacccggt
      781 tgtccttgat gcggcagaca aaggtggaga gggtagactt cgtttctact gcaccaggag
      841 gcacaaagag agaggggtta ggtcgtctcg tgggccataa gacccggcaa aacatgcccg
      901 gtagaacctt ccagccggga ttgaggtaat tgagttcaac aggcatggtg cgtgtctttt
      961 gctccagggc gttgcccagg cgggccacgg tgccgacaaa cttggtaccg ggatgggtgg
     1021 agacggtgaa ttccaccggt gctcccggta gtattccacc ggcattagct tccggcaccg
     1081 gtgtgacgat tctaagtagt cccagttctt gcactctcac tatgggcggg taggcactct
     1141 tgcccagggg accgacaaag ctgcccacat gcatgttgcg ttcagtcaca tagccgtcga
     1201 agggagcgcg cacattcagg tagtccgcta tgtctttata gttttcgctg ttttttctgg
     1261 cggcgtcgag ggcatcaata agcgatgtca ctgcattttc ggcggcattt acattttcct
     1321 gccaggcatt tactttctct ttgtcacttt caaccttttg ctccagcaca attacttcgt
     1381 tgggagccac cacccccggt actagagaag cttccttggt gcgcctgtat gtgtcggaat
     1441 cagccgccag taatgctttc gcttccagta gggtggccct ggctgctgcc agtcttgaac
     1501 gggcttcctg caattcattc tgcacagcct tagacttggc
//
LOCUS       DAOQNZ010001115         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65296_length_1540_cov_3.298990, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001115 DAOQNZ010000000
VERSION     DAOQNZ010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65296_length_1540_cov_3.298990"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1540
                     /locus_tag="PKW73_16915"
     CDS             <1..>1540
                     /locus_tag="PKW73_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75035.1"
                     /translation="DTLESVTGKWQQDFSDLPSIVSRERARFDVTGEKMNALLDRYGF
                     PRRNGDLDTEAFANLVKDSPSDKADFENYLAARKSYSEAVKLQNRALDSRVNDVQWVM
                     DEFTQANGLPKVSVKAGQGHYMSGAAASYRPGEITLHPDQLLGKGNTLDLIEAGYHEL
                     THHEQNFTIVRKLADELKIGNSANISEAEMAQLRQLFKERTNQAMPESVAKEMLAARS
                     KLEPLALTAAEKARAGALEQAIAKNAPAGEKLVELGNDFRTIKSLLKPFQLGKDPNAA
                     FKLLQRLNENGSEALNQRLFGTPTPPNAVQVFADRVEAHLKYGTDSWTKQEATAATKL
                     LQETMEKRLKEINLSRQEVYRDYMRTHEVDAFVSGQRARASGIDRGYKPVEPPKPVRS
                     DHEADGLLLENLGRSSSDQANLHLESLGKDVTGGRISKPRLSFEGGSGQVSTYDLASD
                     TVTFDHASMTVKYPANKLKTELRRPEGFREGEVFTIRGLTEDMPGPVRVKVVTANSER
                     VSLKY"
BASE COUNT          428 a          452 c          411 g          249 t
ORIGIN      
        1 gataccctgg aaagcgtcac cggcaaatgg cagcaggact tctccgacct accctcaatc
       61 gtcagccggg aaagagccag atttgatgtc accggcgaaa aaatgaatgc acttctggac
      121 cgttacggct ttccccggcg caatggcgac ctcgacaccg aggccttcgc caatcttgtg
      181 aaggacagcc ccagcgataa ggccgacttt gaaaattacc tggcggcaag aaaatcatac
      241 tccgaagccg tcaaactcca gaacagggcc cttgatagca gggtcaacga cgtgcaatgg
      301 gtcatggacg aattcaccca ggctaacggc ttaccgaaag tgagcgtaaa agccggtcag
      361 gggcactata tgagcggcgc tgccgcctct taccgccccg gcgaaatcac cctgcacccc
      421 gaccaattgc tgggcaaggg caacaccctc gacttgatcg aagccggcta tcacgagctc
      481 acccaccacg agcagaactt caccatcgtt cgaaaactag ccgacgagct gaagatcggc
      541 aattccgcga acatcagtga agctgaaatg gctcaactgc gccaactctt caaagaaaga
      601 accaaccagg caatgcccga aagcgttgcc aaagaaatgc ttgcagccag aagcaaatta
      661 gaaccactgg ccttgactgc ggcagaaaag gccagggccg gcgccctgga gcaagccata
      721 gccaagaacg caccggccgg cgaaaagcta gtcgagttgg gtaacgactt ccgcaccatc
      781 aaaagcctgc tcaagccctt ccagctcggt aaagacccca atgccgcctt caagcttttg
      841 cagaggttga atgagaatgg aagcgaagcc ctcaatcaac gtcttttcgg tacgccgacg
      901 ccaccaaatg cagtgcaagt ctttgccgac cgagtggagg ctcatctcaa atacggcacc
      961 gactcctgga caaaacagga agccacggca gccaccaaac tcttgcaaga aaccatggag
     1021 aagcggttga aggaaatcaa cctcagtcgt caggaagtct accgagatta tatgcgcacc
     1081 catgaagtgg acgcgttcgt gagcgggcaa agagcgcgag caagcggcat cgataggggc
     1141 tacaagccgg tggagccgcc aaagcctgtc aggtccgacc acgaagcgga tgggctcttg
     1201 ctcgaaaacc tgggcagaag cagctctgat caggccaatc tacatttgga gagcctgggc
     1261 aaagacgtca caggcggaag aatcagcaaa ccgcgactat cctttgaggg cggcagcggc
     1321 caggtcagta cctacgacct ggcatccgat acagtcacct tcgatcatgc ttccatgacg
     1381 gtcaaatatc ctgccaacaa attgaagacc gaactgcggc gcccggaggg cttccgggaa
     1441 ggggaagtgt tcactatccg cggtctcacc gaggacatgc ccggaccggt gcgagtaaaa
     1501 gtcgtgaccg ccaactccga aagagtcagc ctcaagtacg
//
LOCUS       DAOQNZ010001116         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65361_length_1539_cov_4.354447, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001116 DAOQNZ010000000
VERSION     DAOQNZ010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65361_length_1539_cov_4.354447"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1285)
                     /locus_tag="PKW73_16920"
     CDS             complement(125..1285)
                     /locus_tag="PKW73_16920"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNA75036.1"
                     /translation="MLDSVFERSSDSLLQVLQLALDECINLEHTYICTDHILTALVSE
                     YAGVAAEALGSMKIDADAVSKELEGKVKSKMESELPFSGRPDLSSGLPSVTLPVVAQS
                     AGSIAETRKAYGMSDLTVQALHRAYEYALFFGLNYINPEHLLLGILDLKECIAVRTIV
                     DLGGNIDFLRRQIMLLMAKSLSSDASLPGLKDAVIGGLKEMIDKHYRSISTLQALSVR
                     SRHALGSLPPRSEIVHMVCTGYFPDFLINQMAFRRYLLEETLAQLNCRVGGLEKELSA
                     TIISSAAHSLRQEVRNAIEFMLSSEYRLFSQMLDDAEHDLIGSVIEDLWWAQGEEIAL
                     NELFSEALDDHRRKHLLSLQERRLEITSRLSKLRDSLDETIRQCFVKRSATA"
BASE COUNT          401 a          406 c          400 g          332 t
ORIGIN      
        1 gtcctgcccg cagtgcggaa acaccatgcg ctgccgtatc caggaaggtc tcgacatcga
       61 cgagcagcct atctacttcg ccaaagcctt ctgccagaaa tgccctttca ctctcaaata
      121 gagctcaggc cgtagctgaa cgtttgacga aacattgcct gatggtttca tccaggctgt
      181 ctctcagctt ggaaagacgg cttgtaatct ccaatctgcg ctcctgcaaa ctgagcaagt
      241 gtttacgcct gtgatcatcg agcgcctcgg agaaaagctc gttcagggca atttcttcac
      301 cctgagccca ccacaggtct tcaatgaccg aaccaatcag atcatgttcg gcgtcatcga
      361 gcatctgaga aaacagcctg tactcactgg aaagcatgaa ctcgatggca ttgcgtactt
      421 cctgcctgag gctgtgtgcg gcagaagata tgatggtggc tgatagttcc ttttccagac
      481 cgccgacacg gcaattcaat tgagccagtg tttcttccag aagataacgg cggaaagcca
      541 tttgattaat aaggaagtcg gggaaataac cggtacaaac catatgcact atctcggagc
      601 gcggcggcag cgaaccaagg gcatggcggc tgcggacaga aagcgcctgg agtgtgctga
      661 tagagcgata gtgtttgtcg atcatctctt tcaagccgcc aataacagcg tctttcagac
      721 caggcaaaga agcatcgctg ctgaggctct tagccataag caacatgatc tggcgccgca
      781 gaaaatcgat attaccgccc agatcgacaa tggtgcgcac ggcaatgcat tctttcaagt
      841 cgaggatacc gaggagcaga tgctcgggat ttatgtaatt caagccgaag aagagggcgt
      901 actcgtaagc tcgatgcagc gcctgtacgg tcaggtcgct cataccgtag gctttgcgag
      961 tctcagctat ggacccggcc gactgtgcca ccacaggaag tgtcaccgaa ggcagaccgg
     1021 aagaaagatc ggggcgtccg ctaaagggca gctcggactc cattttgctc ttgactttgc
     1081 cttccaattc tttactgacg gcatcggcgt caattttcat gctgcccagc gcttccgccg
     1141 ccacgccggc gtactcgctc accagcgccg tcaaaatgtg atcggtgcaa atgtatgtat
     1201 gctctaaatt gatgcactcg tctaaggcta gctgcaagac ctgcaataga ctgtcactgg
     1261 agcgctcgaa aacagaatcc aacatatgaa atccctgatt ggtagtgcct tgaaaagcgg
     1321 tgcccagcgg ataacccgag agcgcgcaaa atagctatat aattgtaggt catttggcgc
     1381 cgttccaggc agaaccaaag caatttagga aagcttttac ccgggcggca ggctcaagca
     1441 ggaagcaaaa ggaaacagga agcaaaagga agcagctggt catgagaatt cttctatcca
     1501 atgacgacgg tatatacgca cccggcctgg gcaccctgg
//
LOCUS       DAOQNZ010001117         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65494_length_1538_cov_2.765341, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001117 DAOQNZ010000000
VERSION     DAOQNZ010001117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65494_length_1538_cov_2.765341"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1538
                     /locus_tag="PKW73_16925"
     CDS             <1..>1538
                     /locus_tag="PKW73_16925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA75037.1"
                     /translation="YEVLERLGRGGMGTVFKARHKDLDEVVAIKVINPELVEKEGEAT
                     SRRFLAEAKAASRLNHPNLIGLKDYGVTSEGAAYMVMEFVQGESLEDYLKRRGKLEVN
                     EALQIALGICDGLTEAHSKGLVHRDVKPANVILSCAAGAEGQAGINFQPKILDFGIAR
                     ISTADGRTQGLTATGEVFGSPLYIAPEQSMSSKVDQRADLYSLGCLLFEMVTGRPPFL
                     GENAIQTIMMHLNSPVPSATLTLGYALPGGLSQVIARCMDKTPARRYQSVAELKQELE
                     LILSGKSASVNTAPGVKGGSGEDGRSGKFEQARKVARPASAASEKAGRLVAFGIIATV
                     SLTGLLVASQFVPFFFKGGSTGAVGNVNQAFKGGAGAITADELVEQAKQMDMTKALEA
                     YQKGDYQSSAIMLKGAVSVYDDALIKVKKELERTKRGRRRQVLREYIAAVESLKAENL
                     NHVGECYKHMGRAEDAIQNYRNAIEVFKPYSMNGTANPYIDNSYQSSIELLKQSGQTE
                     AARL"
BASE COUNT          378 a          343 c          465 g          352 t
ORIGIN      
        1 gtatgaggtg ctggagcggc tcggacgagg cggcatgggt acggtcttca aggccaggca
       61 caaagatctt gatgaagttg tggccattaa agtaataaat ccggagttgg ttgagaaaga
      121 aggagaagcc acttcccggc gctttctggc ggaagcaaag gcagccagcc gtctcaatca
      181 cccaaacttg atcggtctga aagattatgg tgtcaccagt gaaggtgccg cctatatggt
      241 catggagttc gtgcaaggtg agagcctgga agactacttg aagcggcgcg gcaagctgga
      301 agtaaatgaa gctctgcaga ttgccctggg catttgcgac ggtttgaccg aagctcatag
      361 caaagggctg gtgcaccgcg acgtcaaacc ggcaaacgtg atattgtctt gcgcagccgg
      421 tgctgagggc caggctggga ttaactttca gcccaaaatt cttgacttcg gtatcgctcg
      481 gatttccact gccgatggtc gcacccaggg gttgacggct accggggagg tctttggcag
      541 tccgctctat attgcgccgg aacaaagtat gagcagcaaa gtcgatcagc gggccgactt
      601 gtattcactg ggttgccttt tgtttgaaat ggtcactggt cgtcctcctt ttcttggtga
      661 gaacgccatt cagaccatca tgatgcattt aaactcgcct gtgccgagcg ccacgctcac
      721 gctgggctat gcccttcccg gcggactgtc tcaggtgatc gctcgctgta tggacaagac
      781 accggccagg cgttatcaaa gtgtggctga gttgaagcag gagttggagc ttatcttaag
      841 cggtaagtcg gcatcagtga atactgcccc gggtgtgaaa gggggttccg gagaagatgg
      901 acgcagcggg aaattcgaac aggctagaaa agttgccaga ccggccagtg ccgccagtga
      961 gaaggcggga agactggtgg ctttcgggat cattgcaacg gtatccctga caggtcttct
     1021 ggttgcttct cagtttgtac cgttcttctt taagggcggc tcgaccggtg cggtgggtaa
     1081 tgtcaatcag gctttcaaag gaggagccgg tgctattacc gccgacgagt tggtagagca
     1141 ggccaagcaa atggatatga caaaggcttt ggaagcctat cagaaaggcg attatcagag
     1201 ttctgccatc atgctcaagg gtgccgtaag cgtttacgat gatgccttaa tcaaggtcaa
     1261 gaaggagttg gagcggacaa agcgtggaag gaggaggcaa gtactgcggg agtacattgc
     1321 cgccgtggag tctctcaagg ctgagaatct aaatcatgtg ggtgagtgct acaagcacat
     1381 gggacgggcg gaagatgcta tccagaatta tagaaacgca atagaagtat ttaagcctta
     1441 ttctatgaat ggaacggcta atccatacat cgataattct tatcaatcct ctattgaact
     1501 tcttaagcag agcgggcaaa ctgaagcagc tcggcttg
//
LOCUS       DAOQNZ010001118         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65543_length_1537_cov_4.562078, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001118 DAOQNZ010000000
VERSION     DAOQNZ010001118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65543_length_1537_cov_4.562078"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..623
                     /locus_tag="PKW73_16930"
     CDS             231..623
                     /locus_tag="PKW73_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNA75038.1"
                     /translation="MKQEKLKTPSRLALILALAWFPFSSAAAADAQGLLRYDFRLEHI
                     SCARCILNVRQALRKTAGVQKCEIALRKPYGAVLIVDGAKVNEALIKKVVDSADPGTH
                     PQAVDFLSEPVKAVPAVLLPKHNGLKKN"
     gene            complement(639..1010)
                     /locus_tag="PKW73_16935"
     CDS             complement(639..1010)
                     /locus_tag="PKW73_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75039.1"
                     /translation="MIETVCGSIILIIVALFLVDVAAVVVCQTQNDALAKHCARAAAN
                     KDNAGAANSAVAQVVAEFQKTGGGSKICVYNNYNLTYANAQAYVQTTVTCNFPVPVPL
                     GPSSLQFRADALEPVVAVLPP"
     gene            complement(1089..>1537)
                     /locus_tag="PKW73_16940"
     CDS             complement(1089..>1537)
                     /locus_tag="PKW73_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75040.1"
                     /translation="GGSQIAELAPSLIILLLVILFPMIDIIYLGVGYCSGMYLNQMTT
                     RACSTSKPSDWAAARNAMNSAWLSSSLPHFTGSSIIKNDFKAPQTLAGGDEFDQFVEV
                     NTQVQVSPFISLASVPFLSALSIDGLTKPVVFNYIDKRPMEETGKN"
BASE COUNT          361 a          378 c          415 g          383 t
ORIGIN      
        1 agatggcgct gggagagaag cccgaaagaa ttagttgccc aagaaaatcg cccagcgggt
       61 agagaagtag accgaccagt aagttgatgc gattttgaat ttgttctttt gcctgcattt
      121 atgcctggta ttgtggctat tgctcctcaa gtctgtcaag ttgatcaaat cttgctataa
      181 ataagttggt tggttcaatc ttttacttct aaagtagcaa aggtaaaatc atgaagcagg
      241 aaaaactcaa aactcccagc cgcctggctt tgattttggc gcttgcctgg tttccttttt
      301 cttcagcggc tgccgccgat gcgcaggggc tcttgcgtta cgattttcgt ctggaacata
      361 ttagttgtgc ccgctgtatc ttgaatgtgc ggcaggcttt gcgcaagacc gccggtgtgc
      421 aaaaatgcga gattgctttg cgcaagccct acggtgccgt gctcattgta gatggggcca
      481 aggtaaacga agccttgatc aagaaggtgg ttgatagtgc cgaccccggt acccaccctc
      541 aggccgtaga tttcctcagt gagccggtaa aagccgtgcc cgccgtgcta ctaccaaaac
      601 acaacgggct caagaaaaac taaaaccggt tcgtcggatc agggcggtaa aacggcaact
      661 acgggttcca gcgcatcggc gcggaattgc agactggatg gtcccagggg taccggcaca
      721 gggaagttgc aggtaaccgt ggtttgcacg taggcctggg cattggcata ggtcaggttg
      781 tagttattgt agacacagat tttcgaaccg ccgcctgttt tctggaactc tgctaccacc
      841 tgggctacag ccgagttagc cgccccggcg ttgtctttgt tggcggctgc tctggcgcag
      901 tgtttagcca gggcgtcatt ctgtgtctgg catacgacca ccgctgccac gtccaccaga
      961 aagagcgcca ctataatcaa aatgatggaa ccgcatacgg tttcaatcat atttcccgag
     1021 cttgatctac cgggtttttt ggaacgcgtc ttcaacatcg caagctcacc aaatcctctt
     1081 cttttacctt agtttttgcc tgtctcttcc atgggacgtt tatctatgta gttaaaaaca
     1141 accggcttgg tcagaccatc gatactgaga gcgctcaaga agggcacgct agccagagag
     1201 atgaagggcg agacctgtac ctgggtgttt acttcaacaa actggtcgaa ttcgtcgccg
     1261 ccggccaggg tctgaggcgc tttgaagtcg ttcttgatga tgctggaacc tgtgaaatgc
     1321 ggcagggaac tgctcagcca ggccgaattc atggcatttc tagcggcggc ccagtcggac
     1381 ggcttactgg tggaacaggc cctcgtggtc atctgattga ggtacatgcc gctgcaatag
     1441 cccactccca ggtagataat gtcaatcatc ggaaagagaa ttaccagaag aagaattatc
     1501 aaggacggag ccagttcggc aatttgtgag ccccctg
//
LOCUS       DAOQNZ010001119         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65578_length_1537_cov_2.953441, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001119 DAOQNZ010000000
VERSION     DAOQNZ010001119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65578_length_1537_cov_2.953441"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PKW73_16945"
     CDS             complement(<1..1377)
                     /locus_tag="PKW73_16945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA75041.1"
                     /translation="MGPLALLVAVISGGGLVGWLFGVHSLLEPFVGAKTMSPCTALVC
                     FITSNYLLHWHYFAKHLNMKLPRLLLGVFQIAVGCLVLMHYLHFNTPDFEELLFPLQV
                     SESSDGAQSRMSPTTAFNFMCLGLSFVYLHKRVRTIYIAQLILLFLISISMVCLIGYL
                     FGEPRLYSVGAYMQMSFRTSVCFLLLGLALLFAWPTYTVSAIFANSRLGGYVARRLLP
                     LSLLMAVLGGVLCLFGEQYKLWSWSLGSAAQAVFEATFSSCLVAAIAVSLNKIERERE
                     DLLRRKEKLAAQRDNFMAVLTHDLKNPLIGSEQVLTALINGQTGHLNAEQTHVLELVK
                     RSNHELILMVQNLLALYQYDRDASVFEFAEMELEVPVYEAVAGLKQLASLRNIEIEVN
                     VHQHLPKVRGDAAALCHVVANLLQNAIKFSPEVGAKIVIELLPKDERVLVRVQDQGPG
                     MADEAREKL"
BASE COUNT          455 a          412 c          362 g          308 t
ORIGIN      
        1 aagcttttcc cttgcttcat cggccatacc cggtccctga tcttgcaccc gcaccagcac
       61 tcgctcgtcc tttggaagca attcgataac aattttcgcc cctacttccg gggaaaactt
      121 aatggcgttt tgcaaaagat tggccaccac gtggcagagg gcagcagcat cgcccctcac
      181 tttcggtaag tgctggtgga cgtttacctc tatttcaata ttgcgcaggg aggccagttg
      241 tttgagaccg gctacagcct cgtaaacagg cacctccagc tccatttcgg caaactcaaa
      301 gacactggca tcacggtcat actgataaag tgcaagcaag ttctgcacca tcaatatcaa
      361 ttcatggttg gaccgtttca caagttccaa aacatgggtt tgctctgcat taagatggcc
      421 cgtctgacca ttgataagag cggttaaaac ctgctcactg ccaattagcg ggttcttcaa
      481 atcatgggta agcacggcca taaaattatc ccgttgggcg gccagctttt ccttacggcg
      541 caacaagtct tccctctcac gctcgatttt attgagggaa actgctatag ccgccaccag
      601 gcaactggag aaagtggctt caaaaactgc ctgtgctgcc gaacccaggc tccagctcca
      661 gagtttatac tgctcaccga aaagacaaag tacaccgccc agtacagcca ttaataaaga
      721 aaggggcaag agtcggcgcg ccacatagcc gcccagcctg ctgttggcaa aaatggcgga
      781 caccgtgtaa gtgggccagg caaaaagaag agccagacca agaagaagaa agcagactga
      841 ggttcgaaag gacatttgca tgtaagcgcc gacgctatac aagcggggct cgccgaacag
      901 atagccgatc aagcacacca tggaaatgga tatcaagaaa agcagaatta actgagctat
      961 gtagattgtt cgcactcgct tgtgcaaata gacaaaggaa aggcccaggc acatgaagtt
     1021 aaaggctgta gtaggcgaca ttcgactttg agcgccgtca ctcgactctg aaacttgaag
     1081 agggaagagc aattcctcaa aatccggagt attaaaatgc aaatagtgca tcagtaccag
     1141 acatccgacg gcaatctgaa aaacacccag aagcaatctg ggtagcttca tattcaggtg
     1201 tttagcaaaa taatgccagt ggagcaaata gtttgaggta atgaaacaaa ccagagccgt
     1261 gcaaggcgac atggtcttgg ccccgacgaa aggttcaagc aaggaatgta cgccaaacag
     1321 ccagcccaca agcccgccgc cggaaatcac cgccaccaga agggcaagcg gacccacaag
     1381 cccaataaac ctggcctcga ccgaaccaga acccgtactc gaatcagtca ccataagtag
     1441 tatcgcggca aactatcttc attcccatcc tcaaagcacc tccgcaaccg atttaggact
     1501 gaggggcagc tcaatcctga aggtagagcc tgcacca
//
LOCUS       DAOQNZ010001120         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65638_length_1536_cov_3.869007, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001120 DAOQNZ010000000
VERSION     DAOQNZ010001120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65638_length_1536_cov_3.869007"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKW73_16950"
     CDS             complement(<1..795)
                     /locus_tag="PKW73_16950"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA75042.1"
                     /translation="MKVKWPLNSRATKIFYSAFLPAFFYWLLSPRQSMALYRPLLFHP
                     NPLPESLNSEMLIEVYGRLPEEVWLACGRQRLHGYYFPLSEALYTVLIHHGNSGNMLD
                     RAKLVRWLLSLNLSVFIYDYRGFGGSSGLTSVKTIVEDGLCAFDFLTGRGIPESSIVL
                     YGESLGVAVSANVASLRKPAALFLQSGFSSLRQVALETFPLLGLYPQKLYPKPTLNSA
                     SLLAKGHPPLLIAHGKLDPSVSVSHAYKLYDAASGDKELLIVEEGLH"
     gene            complement(785..1360)
                     /locus_tag="PKW73_16955"
     CDS             complement(785..1360)
                     /locus_tag="PKW73_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNA75043.1"
                     /translation="MDQSARSKTVDLGNFLLRAARLEDVPAISSIYNYYVKTSSASFS
                     VEEETPEKRIHWFLEQKKRNMPVLVAVSKVDGQVCGYAFAAPFRDRPAYSNTVEVSIG
                     FAKQFTGHGLGRPLLQHLIELCKSHYRLMLALVASDNEGSLRFFQACGFRKVGVMTAA
                     VHKFDRYLDLAIFELDLNSEPLEQRETAHES"
     gene            complement(1344..>1536)
                     /locus_tag="PKW73_16960"
     CDS             complement(1344..>1536)
                     /locus_tag="PKW73_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75044.1"
                     /translation="HCRTRYHAVLALICSENDASLKLFASRGFKQVGRLQEVGYKFDR
                     YLDVSILQLILPNSNGSKR"
BASE COUNT          416 a          373 c          419 g          328 t
ORIGIN      
        1 atggagacct tcctctacga tgagcagctc tttgtcaccg ctggcggcat catagagttt
       61 gtaggcatgg ctgacggaaa ctgagggatc cagcttaccg tgggcaatca agaggggcgg
      121 atgtccttta gcaagcaagg aggcactgtt gagggtcggc ttcggataaa gcttctgagg
      181 ataaaggcca aggagcggaa aggtttcaag ggccacctgc ctgagggagc tgaagcccga
      241 ttgcaagaac agggcggccg gcttgcgcag gcttgctacg ttagccgaaa cggccacacc
      301 cagagactct ccatagagga cgatggagct ttcgggtatg ccgcgaccgg taagaaagtc
      361 gaaggcgcat agcccgtctt ccacaatagt cttgaccgag gtcagaccgg aactgccgcc
      421 gaagccgcga tagtcataaa tgaataccga cagattcaag gacaaaagcc agcgtaccaa
      481 tttagcacgg tcaagcatat tgccggaatt gccgtgatga atcaagaccg tatacaaagc
      541 ttccgatagc gggaagtagt agccgtgcag gcgctgtctg ccgcaggcaa gccagacttc
      601 ctcgggcaga cgaccataga cttcaatgag catttcactg tttagcgatt caggcaaagg
      661 gtttggatga aagagcaggg ggcggtaaag cgccatagac tgcctgggcg aaagcagcca
      721 gtagaaaaag gccggcaaaa aagccgagta gaaaatttta gtcgctctag aattgagagg
      781 ccacttaact ttcatgggct gtctctctct gctccaacgg ctctgaattc agatcgagtt
      841 caaaaatggc cagatctaaa taccgatcaa atttgtgcac ggcagccgtc ataactccca
      901 cttttctaaa accgcaagcc tggaagaatc gcagactgcc ttcattgtct gaagcaacca
      961 gggcaagcat cagtcggtaa tgacttttgc aaagttcaat caggtgttgc aacaaaggac
     1021 gccccagacc atggccggta aattgttttg caaagccgat agacacttcc acagtgttac
     1081 tgtaagccgg tctatcgcga aagggagccg caaaggcata accacaaacc tgcccatcta
     1141 ccttggagac agccaccagg acgggcatgt tccgtttctt ttgctcaaga aaccagtgga
     1201 tgcgtttttc gggagtttct tcctccactg agaagctggc gctggaggtc ttcacgtaat
     1261 agttatagat ggaagagatt gcaggtacat cttcgagcct ggcagccctg agcagaaaat
     1321 ttcctaaatc gacggtctta gacctagcgc tttgatccat ttgaattcgg cagtatgagc
     1381 tgaagtattg atacgtccag gtagcgatcg aacttgtagc cgacttcctg caagcgaccc
     1441 acttgcttga aaccgcggga ggcaaagagt ttgagactgg cgtcattttc cgagcatatg
     1501 agagccagga ccgcatgata gcgagttcga cagtgt
//
LOCUS       DAOQNZ010001121         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65646_length_1536_cov_3.459149, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001121 DAOQNZ010000000
VERSION     DAOQNZ010001121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65646_length_1536_cov_3.459149"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PKW73_16965"
     CDS             complement(<1..973)
                     /locus_tag="PKW73_16965"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA75045.1"
                     /translation="MSTAVSENDVLPFGEFLTEEHHAIREAIRDFATREIAPKAHEVD
                     QEARFPIETFKELGKLGYLGLPIGSEYGGAGADYRSYVIAVEEIGRACGSTGLSYAAH
                     VSLGTNPIYKFGTEEQKKKFVPKLCSGEYIGCWALTEPGTGSDASAQKTTAKLVGDHY
                     VLNGTKQFITNATDADVAVIMAMTDMSLGRKGISSFIVEKGTPGFYVSKVEKKLGMRG
                     SPTASLTFEDCKIPVSNMIGQEGEGYKQALMTLEGGRLSIGALALGIGQAALDAALTY
                     AKQREAFGQPIGKFQMIQSYLADMATYVSAARLMLYHAAWMKDHGKRV"
     gene            complement(1060..>1536)
                     /locus_tag="PKW73_16970"
     CDS             complement(1060..>1536)
                     /locus_tag="PKW73_16970"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNA75046.1"
                     /translation="GQGMDEIALALRHIAEEFPDVQVLYPIHRNPVVRQSIEPVFAGH
                     DRLLLVEPLDYVPFVYAMRRCHFILTDSGGVQEEAPALGKPVLVMRTNTERPEAVSAG
                     AAQLVGVSQETICDGARQLMTSPQRYKAMSSAVNPFGDGQAAKRIVQEVEKFLRSP"
BASE COUNT          346 a          420 c          422 g          348 t
ORIGIN      
        1 tgacgcgctt gccgtggtct ttcatccagg cggcatgata gagcatcagt ctggcagcgc
       61 tcacataggt ggccatgtcg gccagatagc tctgaatcat ctggaattta ccgatgggct
      121 gaccaaatgc ttcacgttgc ttggcatagg tgagggcggc atcgagggcg gcctgaccga
      181 taccaagagc gagagcgccg atggagaggc gtccgccttc gagggtcatg agcgcttgct
      241 tgtaaccctc gccttcctga ccaatcatgt tggaaacggg gattttgcaa tcttcaaaag
      301 tcaagctggc ggtgggggag cctctcatac cgagcttctt ctctactttc gaaacataga
      361 agccgggggt gcctttttcc acgatgaagg aggaaattcc tttgcgaccc agggacatgt
      421 cggtcatggc catgattacg gccacgtcgg catcggtggc attggtgatg aactgcttgg
      481 tgccgttcaa aacatagtga tcgccgacta gtttggcggt ggttttctgg gcggaagcat
      541 cagagccggt gcccggttcg gtcaaagccc agcagccgat atactcacct gagcagagct
      601 tgggcacgaa ttttttcttc tgttcttccg taccgaattt atagatggga ttggtaccga
      661 gagagacgtg agcggcatag ctcaaaccag tggagccgca ggcgcgaccg atttcttcaa
      721 cggctatgac atagcttcta tagtcggcgc cggcgccacc atattcgcta ccaatcggca
      781 aacccaaata accaagtttg cccagttcct tgaaagtttc gatcgggaaa cgcgcctcct
      841 gatctacttc gtgagccttg ggagcaattt ccctggtggc gaaatcacga atcgcttcgc
      901 gaatggcatg gtgctcttcc gtcaaaaact cgccaaacgg caagacgtcg ttttcagata
      961 ctgctgtact cacagttctt tctcccgtaa ataatcgata acgaagacca agtcttcgag
     1021 caagatcata gcgctaaacg gctatatcct ggctatttct taggggctcc ttaagaactt
     1081 ctccacctct tgcacaatgc gctttgccgc ctggccgtca ccaaagggat tgacggccga
     1141 agacatagct ttgtagcgct gcggagatgt catcagctgc cgggcaccgt cgcagatagt
     1201 ctcctggctg acgcccacca attgcgccgc tccggcactg acggcttccg gccgctcggt
     1261 gttagtgcgc atgacaagca caggcttgcc aagggcaggt gcttcttcct gaacaccgcc
     1321 cgaatcggtc aaaataaagt ggcagcgccg catggcatag acgaacggca catagtcaag
     1381 gggctcaacc aggagcagcc tgtcatgtcc ggcaaagacc ggctcaatgg attgccgcac
     1441 caccgggttt ctgtggatgg gatagaggac ctgcacatca ggaaattcct cggcaatgtg
     1501 ccgcagagca agcgcgattt cgtccatacc ctgacc
//
LOCUS       DAOQNZ010001122         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65722_length_1535_cov_3.688514, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001122 DAOQNZ010000000
VERSION     DAOQNZ010001122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65722_length_1535_cov_3.688514"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKW73_16975"
     CDS             complement(<1..689)
                     /locus_tag="PKW73_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75047.1"
                     /translation="MKATNLILVLSACSNLSLLYQAPPATAAPAAPPAAPVRKPLSTA
                     GRLTAAEAREANALTELCKQGKLKEAAPRLRLLAEKHPNVLSIQLNMAQLMLILGESQ
                     TALSAASRAVKLDPTNAHAWRYFSEANMHLGKQEEAVQAMKKAVAFCQSPALKKDLQE
                     TLTAMNKEKARNAAVKSKVYAPGSYIDEESLSMMPRWSKERMPLAVFISPGNVKGYND
                     QLKELLKEAFA"
     gene            854..>1535
                     /locus_tag="PKW73_16980"
     CDS             854..>1535
                     /locus_tag="PKW73_16980"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA75048.1"
                     /translation="MSKKNSDKDADRAYRMRTKLIHGTFHSKCWDYNHHIIPPMSASA
                     AYRLDSVHRGAQGFTEFAAEGMEENQPIYIYDRLQEPTRGLLEENLSSAEGGEMAVCF
                     STGMAAISAAVGILTGAGDEILAHHTLYGCTYSLFTNWLPRYNIKTSFCDLRNLEETA
                     ALINENTRVVYLESPVNPTLDLIDLAALCRLVEKCNQERVKERRIKVIVDNTFATPVC
                     QRPLGLGAD"
BASE COUNT          326 a          419 c          408 g          382 t
ORIGIN      
        1 tcggcaaagg cttctttcag aagttccttc agttggtcgt tataaccttt gacattacct
       61 ggtgaaatga agaccgccag cggcatgcgc tccttcgacc agcggggcat catgctcagg
      121 ctttcttcat cgatgtaaga gcccggggca tagaccttgc tcttaacggc ggcattcctg
      181 gctttctcct tattcatggc cgtcagggtc tcttgcaggt ctttcttgag agcgggcgac
      241 tggcaaaatg ccaccgcttt cttcatggct tgcactgctt cttcctgctt acccaggtgc
      301 atattggctt cactgaaata acgccaggca tgggcattgg taggatcgag tttgacggcc
      361 cgacttgcgg cgctcagggc agtctggctt tctcccagga tgagcatgag ctgggccata
      421 ttaagctgta ttgaaagcac gttcgggtgc ttttccgcca gaagtctaag gcgcggcgcc
      481 gcttccttca attttccttg cttgcatagc tccgtcaggg cattggcttc cctggcctca
      541 gcggcagtca gtctgcctgc cgtgctgagc ggcttgcgca ctggtgccgc cggtggtgca
      601 gccggggcgg ccgttgccgg cggtgcctga tataaaagag acaaatttga gcaggcactc
      661 aaaaccaaga ttagattagt tgctttcata aagacctgtt tgttctcctc attattgcag
      721 caaccggcat atcagtcttc ccttagcgat ctgctccctt gagatctgtg gatggctatt
      781 atcatgcttg cggtggataa aatcagggtc tctatcggtc aagatataaa gtcgggatgg
      841 caggagtaaa ccgatgagca agaagaactc tgataaggac gccgatagag cctacaggat
      901 gcgcacaaag ctcatccacg gaacctttca cagcaaatgt tgggactaca atcaccacat
      961 tattcccccc atgtcggctt ccgccgctta tcggttagac tcggtccatc gcggcgccca
     1021 gggttttacc gaatttgccg ccgaaggcat ggaagaaaac cagcctatct acatttatga
     1081 ccgtctgcag gagccgaccc gtggtcttct ggaggagaac ctttcttctg cagaaggcgg
     1141 ggagatggct gtctgcttct ccacgggcat ggcggccata agtgctgcgg taggcatatt
     1201 gaccggcgcc ggtgacgaga tcctggctca ccatacgctc tatggctgca cctatagcct
     1261 cttcaccaac tggctgccgc gctacaacat aaagacgagc ttttgtgatc tgcgcaatct
     1321 ggaagagacc gccgctctca taaatgaaaa tacgcgggtg gtttatttgg aatcccccgt
     1381 caaccccacc ctcgatctca tagacctggc ggcactttgc cggttggtgg aaaaatgcaa
     1441 tcaagagcgg gttaaagagc gtcgcatcaa ggtgattgtg gacaatacct ttgccactcc
     1501 cgtatgtcag cgccccctcg gccttggtgc cgatc
//
LOCUS       DAOQNZ010001123         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65773_length_1535_cov_2.195270, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001123 DAOQNZ010000000
VERSION     DAOQNZ010001123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65773_length_1535_cov_2.195270"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PKW73_16985"
     CDS             complement(<1..1117)
                     /locus_tag="PKW73_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HNA75049.1"
                     /translation="MGLSAKITFGFLFVIMFMVCGHAISSTYNDYKNNRETASMKLGE
                     AIKQVKIDLGSILKANEAFAIQMVGDQNLMGPFARGDKAAFGSALKDVVLKTGFSGYV
                     TIIDDKGKVFYSSDSPKQSGYDARANSPGVDFVFRNNKSYGGAASFTQPGTLGLSSMV
                     PIRLGNRVAGVMIASQTLNTEFLTGMTTKLQTMDPGRLAGIDLALVACNGKASKGGQL
                     IAVSPGLVKKSTPFLKSLEEHGIKALPAKSGPLAQFANPNAGFEESGRLWRDLKVEDL
                     NENQSIAILLATTPVENMVQRAVNVTILAASCGALAFLVALLFSAGISKGVNKPLRFL
                     IRRTNELASQRQVLPALEGLSGDWLELGELIDTSVVSM"
     gene            complement(1332..>1535)
                     /locus_tag="PKW73_16990"
     CDS             complement(1332..>1535)
                     /locus_tag="PKW73_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75050.1"
                     /translation="QILVELGYITIYQLDEALQIQREDSAKGVEQKALGQILLELGYC
                     GPNQLIRGIQVQSEYRKVAPSED"
BASE COUNT          357 a          388 c          407 g          383 t
ORIGIN      
        1 gcatagagac gaccgatgtg tcgataagct ctcccaactc cagccagtct ccggagagac
       61 cttccagagc cggcaagacc tgtctctggc tggccaattc attggtcctg cggatgagaa
      121 agcgcagagg tttattgacg cctttggaaa ttccagccga gaaaagtaaa gccacaagaa
      181 atgccagagc gccgcaggat gccgccagga tggtgacgtt gacggcgcgc tgaaccatgt
      241 tttccacagg agtggtggca agaagaattg ctatgctctg attctcgttg agatcttcca
      301 ctttgagatc gcgccagagc cttccgcttt cctcgaagcc tgcatttgga ttggcgaatt
      361 gggcgagggg acctgatttg gctggaagcg cctttatccc atgctcttcc aggctcttca
      421 aaaagggggt cgactttttg accaggcctg gagaaacagc aatcagttgc ccgccttttg
      481 aggctttgcc gttgcacgcc acaagagcca ggtctatgcc ggccaggcgg cccgggtcca
      541 tagtctgaag cttggtcgtc atgccggtta agaactctgt gttgagtgtc tggctggcaa
      601 tcattacccc ggctacacga ttgcccagtc gaatcggtac catactggac aaacccagtg
      661 ttcctggttg agtgaaagaa gcagcgcccc cgtaggactt gttgtttcga aagacgaaat
      721 ccacgccggg gctattcgct cttgcgtcat aaccggactg ttttggcgag tccgagctgt
      781 aaaaaacctt gcctttgtcg tcgatgatcg tgacgtatcc gctaaagcct gtcttgagga
      841 ctacgtcctt cagcgctgag ccgaaagcgg ccttgtcgcc ccgcgcaaat ggacccatca
      901 ggttctggtc gcccaccatc tggatggcaa aggcttcgtt ggcttttaga atagagccca
      961 ggtctatttt gacttgctta atcgcctcgc ccaacttcat cgaggcggtc tcgcgattgt
     1021 ttttgtagtc gttataagtc gaacttatgg catgcccgca gaccatgaac atgatcacga
     1081 acaggaaacc aaaagtaatc tttgcgctta gtcccaatga aaagtcacgc tgtcacacgg
     1141 aggtaaaagc cagaggaact ttttatgaga agctcacaaa aagttttccc attagaagtt
     1201 tatccacttt ctaaagtatc taagcgtgat cagcagaaaa accaagtttc tctgggatag
     1261 atgttaagcg gagaggcaag ccctgtcagt ggggtttacc cggaatctct tggaatggtg
     1321 tcttgcaaag attagtcttc cgaaggtgct actttgcggt actcggactg cacttgaatt
     1381 ccgcggatta gctggttggg tccgcagtag ccgagttcta gaagaatttg gcccagcgct
     1441 ttctgctcaa cacccttggc ggagtcttca cgctgaatct ggagcgcttc atcgagctga
     1501 tagatggtta tgtagccaag ctctaccagg atttg
//
LOCUS       DAOQNZ010001124         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65827_length_1534_cov_3.181880, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001124 DAOQNZ010000000
VERSION     DAOQNZ010001124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65827_length_1534_cov_3.181880"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PKW73_16995"
     CDS             <1..924
                     /locus_tag="PKW73_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75051.1"
                     /translation="AVVKIFRHHRILWKPAMKVALIWLVLGLLDTGNNLINIYESYDT
                     AKPLQNFFLQSAIEKLGLAALQWLGVSLTLVLSLAALPAEVRKRLRACVLMLLPARQE
                     AYTKQHRDYWLDAILLGGTFTVLSGLLSVLSKWMKVLFSHELPCSFATGALADAYNTY
                     FPALSDLTDLLKQAVNQPLIFGAAAALLITLKLRSLKFIIPMVLTLAFMQAVYERHTV
                     DIILKFLSLCLSFSLYYILILTAARRNILTLILYSFFSAALARLTIFLDGGKEVFASD
                     IATVSVLILLPLTYLLYTQRVHELAKRLSRR"
     gene            complement(902..>1534)
                     /locus_tag="PKW73_17000"
     CDS             complement(902..>1534)
                     /locus_tag="PKW73_17000"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNA75052.1"
                     /translation="IFAAERSAWLGMFAGILILTWRMSRRSFLLGIVSLVVATALSYS
                     FVPVVKTRLNSLTDWRRDVSITARLQVWQVAWTTFNRSPWLGVGPRNFPRIENEQAIV
                     KGQSSDLNHGHSNYMHILATTGVLGILTYLVLVLYPFSRLYMAAAPQLSSNSPEYDSG
                     LYLGLLAALASLMVAGIFEYNFGAGSVRLAQWFCLAFYRPPQASICEKDA"
BASE COUNT          360 a          457 c          373 g          344 t
ORIGIN      
        1 gccgtggtga aaattttccg ccatcaccgc attctctgga agccggctat gaaagtagct
       61 ctgatctggt tggtgctagg tctactagat accggcaata acctcatcaa tatctacgaa
      121 agctacgata cggccaagcc gctacaaaac ttcttcctgc aatcggccat tgagaaactc
      181 ggtctggccg ccttgcaatg gctgggtgtc agcctcaccc tcgtcctcag cctggccgcc
      241 ctgccggcgg aagtccgcaa aagactgcgg gcttgcgtgc tgatgttact gccggccaga
      301 caagaagcct ataccaaaca acaccgcgac tactggctgg atgccatcct cctgggcggc
      361 accttcacgg tgctctccgg gctcctttca gtcttaagca aatggatgaa agtgctcttc
      421 tcccacgaac tgccttgcag tttcgccacc ggtgcccttg cagatgccta taacacctac
      481 tttccagcac tatccgatct gaccgactta cttaaacaag ccgtcaatca gccgctcatt
      541 tttggtgccg ccgccgccct gctcattact ctgaaactgc gcagcctcaa gttcatcatt
      601 cccatggtgc tgaccctggc cttcatgcaa gccgtgtacg aaagacatac cgtggacatt
      661 atcctcaaat ttctgtccct ttgcttgagc ttcagtctct actacatcct aattttgacg
      721 gcggcccgcc gtaacatact gacgcttatc ctctacagct ttttcagcgc cgccctggcc
      781 aggcttacaa ttttcctgga cggcggcaaa gaggtattcg cctctgatat tgccacggtt
      841 tccgtcctga ttcttttgcc cctgacttac cttctctaca cccagagagt gcatgaactg
      901 gctaagcgtc tttctcgcag atagatgctt gaggcggacg gtaaaaagcc aggcaaaacc
      961 actgggccag ccgcaccgaa ccggcgccga aattgtactc gaagatacct gccaccataa
     1021 gcgaggcaag ggcggcaagc agacctagat agagcccgga atcgtactcc ggggagtttg
     1081 acgacaattg cggcgcggct gccatataga gcctggaaaa aggatagagg accagcacca
     1141 ggtaggtgag aatcccaagc acgccggtgg tggctaagat gtgcatatag ttgctgtgcc
     1201 cgtggtttaa atcggacgat tgtcctttta caatggcttg ttcattttca attcgcggga
     1261 aattgcgcgg tcccaccccc agccaggggc tgcggttaaa agtcgtccag gctacctgcc
     1321 agacctgaag acgagccgta atactcacat cccgacgcca atccgtcagg ctgttcaggc
     1381 gtgttttcac caccggcaca aaactgtagg atagcgccgt cgccactacc agcgatacta
     1441 taccaagaag gaaggaccgg cgtgacatac gccaggtcaa aataagaatg cctgcaaaca
     1501 taccaagcca ggcgctgcgt tcggcggcaa agat
//
LOCUS       DAOQNZ010001125         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65830_length_1534_cov_3.093982, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001125 DAOQNZ010000000
VERSION     DAOQNZ010001125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65830_length_1534_cov_3.093982"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKW73_17005"
     CDS             <1..453
                     /locus_tag="PKW73_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA75053.1"
                     /translation="AGGYCLKDVLPERLYTAIRSVNAGDIWLDSSIASRVVRLYNSSG
                     FAAGGSKAAANSASESPDTSAHHDAPRAAAHSTHSFSNLPSLPEPLSPRELEVLGLLV
                     DGLSNQEIADKLIISLATAKTHVRNILNKLAVDDRTQAAVQAMRRGLV"
     gene            complement(731..>1534)
                     /locus_tag="PKW73_17010"
     CDS             complement(731..>1534)
                     /locus_tag="PKW73_17010"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNA75054.1"
                     /translation="VGGKRVRGFYVTGLTGSGIAADLSLNSGDVVLTLAGRGVDSGPA
                     VDALLTTTVAGNLKATIARVRGGRVTVSNPLISFQGYAPLPKLAAKPTTGTTGGSSGG
                     FGSSMFKDPEDEMGEDAKPKERPSVEALESHMYQLVNADRAANGLPPVARSSALGRVA
                     RTFAEDMAARNYFNHQDPEGRWPKDRAQAAGISAGVWENIGWQKNFNGFTRLIDQVQA
                     NFMNEPKDDPTNHRANILNRGHHCVGIGVAVVMPYSVICVQEFSHDDVP"
BASE COUNT          310 a          429 c          402 g          393 t
ORIGIN      
        1 gctggtggct actgtctcaa agatgtattg cccgagcgtc tctatacggc gatccgctct
       61 gtaaatgctg gtgatatttg gctcgattct tccatcgcca gccgggttgt aagactttac
      121 aacagtagcg gctttgccgc cggcggcagc aaggctgctg ccaatagcgc ctctgagagt
      181 ccggacactt cagctcatca tgatgcgccg agggcagctg cccatagcac ccatagcttc
      241 tccaacttac cctccctgcc tgaaccgctt tcacccagag agttggaagt attggggcta
      301 ctggtggatg gtctttccaa tcaggagatt gccgacaagt tgataatctc cctggccacg
      361 gctaagaccc atgtgcgcaa catcctcaat aaattggctg tcgacgaccg cacccaggcg
      421 gcggtgcaag ctatgcgtcg cgggctggtc tgagtaaagt tccaatctgc atttgatttc
      481 cgggcaaggt atggttggct gcaaatatct gctcctctct tgaacttttt gcttgcagcc
      541 gacgtcttgt tatttgagac tgccggactc tagaggttgg gggagacttc ctcgccggtg
      601 gtcccattaa ttgaacgcgc ctttaaagcc gcccccgcaa gactgacctg agttttgccc
      661 ggtaagaaac ttcagatact taagcaacga aagacatctg aggtatcgaa atagctgata
      721 ctggagctaa ttaaggcaca tcatcatggc tgaattcttg cacgcagata acgctatagg
      781 gcatcactac ggctaccccg atgccgacgc agtggtgccc acggtttaga atattcgctc
      841 ggtggtttgt gggatcatcc ttgggttcat tcatgaaatt ggcttgcacc tgatcgatca
      901 gtctggtgaa gccgttgaag tttttctgcc agccgatgtt ttcccagacg ccggcagaga
      961 ttcccgccgc ctgggctcgg tctttcggcc agcgaccttc aggatcctgg tgattgaaat
     1021 agtttctggc cgccatgtct tccgcaaaag tgcgggcgac tctgcccagg gcactgctcc
     1081 ttgctaccgg tggtagaccg ttagctgctc tgtcggcatt gaccagttgg tacatgtggc
     1141 tttccagggc ttcgacgctt ggcctctcct ttggctttgc atcttcaccc atctcatctt
     1201 cggggtcttt gaacattgaa ctgccaaaac cgccactact accgccggtg gtgccggtag
     1261 taggtttggc tgctaatttt ggcagcggtg cgtaaccctg aaaactgatc agcggattgg
     1321 atacggtcac tcttccgccc ctgactctgg ctatggtggc tttcaaattg cctgccacgg
     1381 tagtggtgag caatgcatct acggccggtc ccgaatcaac ccctcgccct gccagagtaa
     1441 gcactacatc gccgctgtta aggcttagat cggctgcgat accggagccg gtcagaccgg
     1501 tcacataaaa tcctcgtact cgcttgccgc ctac
//
LOCUS       DAOQNZ010001126         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65864_length_1534_cov_2.179175, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001126 DAOQNZ010000000
VERSION     DAOQNZ010001126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65864_length_1534_cov_2.179175"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PKW73_17015"
     CDS             <1..1061
                     /locus_tag="PKW73_17015"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNA75055.1"
                     /translation="ANSARQNSPQTGSDPGAVPDLIQIAAPVAHEMQLVEEWLTTNLI
                     DDSAFVGELLSQLFQAGGKRIRPLISLLASAASLTDEKVDPHHSRMSRLHIILAVLTE
                     LIHAASLVHDDVIDAADVRRGHATVNRRFNDKMAVLLGDLLFAQASICLARIMNPSVV
                     GVYGQVLGDLCSGEISQMRQQFSLKVDFDAYVQKSIGKTASLIAAGAHCGAILNARSN
                     EVIQALHQYGTNLGICFQIVDDLLDVTGSAAKTGKPVGSDLKNGLLTAPSLFILARGD
                     ELSERFAELVKTRKVENEDGLKEALTIVEEGGGIEKTKELAGAYGLKARDALNILPPT
                     PARQSLSDIVDYVLVRSS"
     gene            1104..>1534
                     /locus_tag="PKW73_17020"
     CDS             1104..>1534
                     /locus_tag="PKW73_17020"
                     /inference="COORDINATES: protein motif:HMM:NF024638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA75056.1"
                     /translation="MTFSSRTNSYASYGQSFLPVDLSLKEQVVLVFGGSRAAYLELLK
                     ILDSGARVQLVARVAVSEIQELLLTHAGRLTMTRLDPLAFIRDAERPSLVLIFGLSAE
                     VEASLVEHYRKLSVPLFVQYRPDLSDFSLPTFLKRGYLKVAV"
BASE COUNT          316 a          413 c          412 g          393 t
ORIGIN      
        1 aggcaaattc cgccaggcaa aattcccctc agaccgggtc ggatcctggt gctgtacctg
       61 atttgattca gattgcagca cctgtggccc atgaaatgca gttggtggaa gagtggctga
      121 ccaccaatct gattgacgac agtgccttcg taggtgaatt gctttctcaa cttttccagg
      181 ccggcggcaa gcgtatacgc ccccttattt cgctgctcgc ctctgctgct tctctcactg
      241 atgagaaggt cgacccacat cacagcagaa tgtcgcgcct gcacattatt ctggctgtcc
      301 tgaccgaact gatccacgcc gccagtctgg tgcatgacga tgtcatcgat gccgccgacg
      361 tcagacgcgg ccatgccacc gtcaaccgcc gtttcaacga caaaatggca gtcttgctcg
      421 gcgatctgct cttcgcccag gcttccattt gcctggctcg catcatgaat ccctccgtag
      481 tgggagttta cggtcaggtg ctgggtgatc tttgctccgg cgagatcagt caaatgcgcc
      541 agcagttttc tttgaaggta gacttcgatg cctatgtgca aaagtccatc ggtaagacgg
      601 cttccttaat tgcggccggt gcccattgcg gcgctattct caatgctcgt tccaacgaag
      661 tgatacaagc cttgcaccag tacggcacca atcttggtat ttgctttcag atcgttgatg
      721 accttcttga tgtgaccggc tctgccgcta aaaccggaaa gcctgtaggc agtgacctga
      781 agaatggctt gttgacggct ccgtcccttt tcattctggc ccgcggtgac gaactgtccg
      841 agcgcttcgc cgagctggtc aagactcgca aggtggaaaa cgaagacggc ttgaaggaag
      901 ctctgaccat tgtggaagag ggcggcggca ttgagaagac aaaagaattg gccggtgctt
      961 atgggttgaa agcccgggat gctctcaaca ttctgccgcc aacacctgcc cggcagtcgc
     1021 tttcagatat tgtcgattac gtgctggtgc gctcgagcta gcaggtaaat agaaattttc
     1081 gttgaacggg cgtcaattgc aagatgactt tcagtagccg aacaaattca tacgcctctt
     1141 acggtcagtc ttttctgccg gttgacctga gcttgaaaga gcaagtggtg cttgtcttcg
     1201 gcggcagtcg agcggcctat ctggaattgt tgaaaatact ggacagcggt gcccgtgtac
     1261 agcttgtggc cagagtggcc gtcagtgaaa ttcaggaatt gctcttgacc catgccggtc
     1321 gcttgaccat gacgcgtttg gatccccttg cgtttatcag ggacgccgag cgtccttcac
     1381 tggttctgat ttttggtttg tcggcggaag tggaagcttc cctggtggag cattaccgaa
     1441 agctttccgt acccttgttt gtgcaatacc gaccggacct ctccgatttt tccctgccca
     1501 cctttctcaa acgcggctac ttgaaagtgg cggt
//
LOCUS       DAOQNZ010001127         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_65974_length_1532_cov_3.890318, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001127 DAOQNZ010000000
VERSION     DAOQNZ010001127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65974_length_1532_cov_3.890318"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1532
                     /locus_tag="PKW73_17025"
     CDS             <1..>1532
                     /locus_tag="PKW73_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75057.1"
                     /translation="LNIEDIPLSYQPPVGPGMDFRFNYNHLEANQPSTYSFSNMGQNW
                     TFNWLSYLTVDPTSSVALVRVVGGGSEQYTPDVVTGLYPPALESQAQLVAMGGGVYER
                     RLPDGSVETFNQADSSSPPRIFMTELKDPQGLTCQIQYDSDFRIVSITDAIGQVSSVS
                     YLSNTPGNPGFYKISGVTDPFGRSCSLAYDPSATNLLSITDVINLKSQFAYDNTSSFI
                     TQLSTAYGTTSFYQYVPGVVGGIPAQGLRCTFPDGTSSVIENWLNEAKTTYFWDRHAT
                     QLYPADPANKIYTQSEQTRFTFNLQTGFEGPVKQNETHPLESQSPIFFTYPSPYGPNF
                     QGNSNRPLSITRSLGNPTVVATVGGTITAGDIVYLGVESQYVGAYTVQSGDTTTSIAG
                     SLAGIVNTNATLQGIGVVATAIGPTVALHSDQSGVFSYVAGVSAGATETCKVLSPSRQ
                     SAKATMSGPLTNGDVLYVYVGTPYPHGGTGQRYFHTVAAGDTYNSILNDIATQLNADP
                     IV"
BASE COUNT          372 a          447 c          385 g          328 t
ORIGIN      
        1 ctctcaatat tgaagacatc ccgctcagct atcagccacc agtgggaccc ggcatggatt
       61 tcaggtttaa ctacaaccac ctcgaggcca accagccgag cacctacagc tttagcaata
      121 tgggacagaa ctggactttt aactggcttt cctacctgac tgtcgacccc acaagctcgg
      181 tcgccttagt ccgcgtagtc ggtggcggca gtgagcagta cacaccagat gtggtgactg
      241 ggctatatcc cccggcactg gaatcgcagg cgcaattagt tgctatgggc ggcggtgtct
      301 atgaaagacg cttgccggat ggctcggttg agactttcaa ccaggctgac agcagctcgc
      361 cgccgcgcat tttcatgaca gagctgaaag atccgcaggg gctgacttgc cagattcagt
      421 acgattccga cttcaggata gtctctatca ctgatgccat cggccaggtt tctagcgtct
      481 cttatctctc caacacgcca ggcaatcccg gcttttacaa aatatccggc gtcaccgacc
      541 cattcggacg cagttgttct cttgcttatg acccaagcgc gaccaatctg ctttcgatca
      601 cggacgtgat caacctgaag tcacagtttg cctatgacaa cacatccagc ttcattactc
      661 agttgagcac ggcgtatggc acgacaagtt tctatcaata tgttccaggc gtggtgggcg
      721 gtataccggc acaggggctg cgttgcacct ttccagacgg cacctcgtct gttatcgaga
      781 actggctcaa tgaagcaaag accacatact tctgggatag gcacgccact cagctctatc
      841 ctgccgatcc ggcaaacaag atttacactc aaagcgagca gacaagattc actttcaacc
      901 tgcaaactgg cttcgaaggg ccggtgaagc agaatgaaac tcacccgttg gagagccaga
      961 gcccaatctt ctttacctat ccaagtcctt acggtccaaa cttccagggc aacagcaaca
     1021 ggcccttgag catcaccagg agcctcggaa atcccaccgt ggtagccact gtcggcggca
     1081 ccatcacagc gggagacatc gtctaccttg gcgtcgaaag ccagtacgtg ggcgcctaca
     1141 ccgttcaaag cggcgacacc accacatcga tagctggcag cctggctggc atagttaata
     1201 ccaatgctac cctgcaaggg ataggagtag tggctacggc cattgggccg actgtggcac
     1261 tgcattcaga tcaaagcggt gtcttcagct acgtggccgg ggtatcagcg ggagcgactg
     1321 aaacctgcaa ggtgctctca ccaagcaggc agagcgccaa ggcgaccatg agcggacctc
     1381 tgacaaatgg agatgtgctc tatgtctatg tcggtactcc ctacccacat ggcggcaccg
     1441 ggcaaaggta ttttcacacg gtcgccgctg gtgataccta caattcaatt ctcaatgaca
     1501 ttgccacgca gttgaacgcc gacccaatag tt
//
LOCUS       DAOQNZ010001128         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_66070_length_1531_cov_3.130759, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001128 DAOQNZ010000000
VERSION     DAOQNZ010001128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66070_length_1531_cov_3.130759"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1531)
                     /locus_tag="PKW73_17030"
     CDS             complement(<1..>1531)
                     /locus_tag="PKW73_17030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA75058.1"
                     /translation="DEREPTKARDAANLEKTTVRDSFAEGGQFSRGAIIGERFVVEAF
                     IGAGAVGQVYKVYPLENTQDRASRKGERTYLALKILTGADVSQVAWKRFQTEIEVIAR
                     LTHGNVVRIMDSGIHGQSNPYYTMELVEGESLADKIKSNGPMEITEALKIFICVADAL
                     NEAHKKSILHRDIKPSNIVIATAGSSIYSRVKVVDFGIAKLMGRDLPEGQALTGRGEI
                     FGSPLYMSPEQACGSRLDNRSDIYSLGCTLFEALTGQPPFKGNSIFETLSLHQEAKPP
                     KLSQVAPGKKWPRSLEDLVDQLLSKLPDDRCGTMAQVEEILESVLDELGDSQSVTSYA
                     ALKGKERKPVKKVTTGLVDNDKPTTEDSKVIFSLTIISVLAVLLLGGTAAAIILGSRQ
                     KAPVAVVNETGPIIPDTKSVKEMTRENPDSIEGCKTSGKLPAPVKPGSFFKGLAPSVT
                     GSPSGFDATYTFPAGFDGGCIEASFEEESEFKKKTDGMGVVLRNSSKKGKLRATGTLT
                     L"
BASE COUNT          292 a          409 c          412 g          418 t
ORIGIN      
        1 tgcagagtga gggtgccggt ggctctcagc ttgcctttct tactggaatt tctgagcacc
       61 actcccatgc cgtcggtctt tttcttgaat tccgactctt cttcgaagct ggcttcgata
      121 caaccaccgt caaagccggc agggaaggtg taagtggcat caaaaccgct tggtgagccg
      181 gttacactgg gagccagtcc tttgaagaaa ctgccgggct tgaccggtgc cggcagttta
      241 ccggaagttt tacaaccctc gatggagtcg ggattttctc tggtcatttc tttgactgac
      301 ttggtatcgg gtattattgg tccggtttcg ttcacgactg ccacaggcgc cttctgtctg
      361 gagccgagaa tgatggcagc agccgtgccg cccagtaaca acactgccag cactgaaatg
      421 atcgtcaggc tgaaaatgac cttgctgtct tcggtggtgg gtttgtcatt atccacaaga
      481 ccggtggtta ctttctttac gggcttgcgc tccttacctt tgagggcggc gtaggaggtg
      541 acggactgac tgtctcccag ctcatcaagc acactttcca agatttcttc cacctgagcc
      601 atggtgccgc aacggtcgtc gggcaacttg ctcaataact ggtctaccag gtcttccagg
      661 ctgcgtggcc atttcttgcc cggtgcaact tgtgaaagct ttgggggttt ggcttcctgg
      721 tgcaggctga gagtctcgaa gatgctattg cctttgaaag gcggttgacc ggtaagggct
      781 tcaaaaagtg tacagcccag ggaataaatg tcggagcgat tatcgaggcg gctgccgcaa
      841 gcctgctcgg ggctcatgta gagcggcgag ccgaagattt ctccccgccc ggtcagggcc
      901 tgcccttccg gtaggtcccg ccccattaat ttggcgatgc caaaatcgac tactttgacg
      961 cggctgtata tgctactgcc ggcagtggca atgacgatat tgctcggctt aatgtctcgg
     1021 tgtaagatgc ttttcttgtg ggcttcattg agggcatctg caacacagat gaaaatcttc
     1081 agcgcttcgg tgatttccat aggaccgttg cttttgattt tatcggccag gctctcgcct
     1141 tccaccagct ccattgtgta ataggggttg ctctgcccgt gtatgccgct atccatgatg
     1201 cgcaccacat tgccgtgggt cagtctggcg attacctcta tttcggtctg aaagcgtttc
     1261 catgccactt gtgatacatc ggcacctgta agaattttga gggccaggta agtgcgctcg
     1321 ccttttctgc ttgctctatc ttgtgtgttt tccaacgggt agactttata tacctgaccg
     1381 accgcccctg cgccaatgaa ggcttctaca acgaagcgtt cgccgattat cgcaccgcgg
     1441 ctgaattgac cgccttcagc aaaactatcg cggactgtgg tcttctcaag gttcgccgcg
     1501 tcccgagctt tagtcggctc cctttcatca g
//
LOCUS       DAOQNZ010001129         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_66658_length_1524_cov_3.215793, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001129 DAOQNZ010000000
VERSION     DAOQNZ010001129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66658_length_1524_cov_3.215793"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..759)
                     /locus_tag="PKW73_17035"
     CDS             complement(73..759)
                     /locus_tag="PKW73_17035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA75059.1"
                     /translation="MLLTGNAAQASGPDQAAREFQKAKQCQKVIDSEGALQHYKKAVE
                     LDPKNSRIYVERGIAYKDLGKFDLALADYNKSLAMAPGNIPALEARARLYDMTGRLKE
                     AIADYDCVLKSPNANWQTWRHRAICNRRLKNYLESAKDFGTALKLAGVCRDQQQMMYY
                     QAEMLFLGNDLPGALSRFDAIIKSFPDLSGAYWSRAKIYDKMGKADLAEKDRQKARKL
                     DEDFDPLNRL"
     gene            complement(801..>1524)
                     /locus_tag="PKW73_17040"
     CDS             complement(801..>1524)
                     /locus_tag="PKW73_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNA75060.1"
                     /translation="LCRPGAKMIFRNRADAGAKLAEKLAALFTASNTIPYCVVGLPRG
                     GVPVALEVAKKLSSPLELIESKKLPYPGQPEFAIGAVSADGIVVLNHDVPGREKMTEG
                     ERTRWHDYIEQQSKLLLEKTKAVEAQFYRLAGRRKASLKGKTVIIVDDGIATGMTALA
                     AVETARKQGAAKVILAAPVMSKESMEELKSHCDSIVTLSVPDPFCAVGLYYQDFSQTR
                     DEEVVQALRDSTGFASTTSQAL"
BASE COUNT          291 a          400 c          410 g          423 t
ORIGIN      
        1 cactagccct tgcggttctg gtgctgtcca gagaagcggc agcggaattg gagcagattt
       61 gttcccgagc aactaaagcc tgtttagggg gtcgaaatct tcgtcgagct ttcgtgcttt
      121 ctgtctgtct ttttcggcca ggtcggcctt gcccattttg tcgtagattt tagctctgct
      181 ccagtaggca cctgataaat cggggaagct tttgatgatg gcgtcaaagc ggctcagtgc
      241 tccgggtaga tcattgccga ggaagagcat ttcggcctgg taatacatca tttgctgctg
      301 gtcccggcag actccggcta gtttcagggc tgtaccgaaa tcttttgccg actccaggta
      361 gtttttcaaa cgccggttgc aaatggcccg gtgcctccat gtctgccagt tggcgttggg
      421 agatttgagc acgcagtcat agtcggcgat ggcttctttg aggcgacctg tcatgtcgta
      481 gagcctggct cttgcttcca gggcgggaat atttcccggc gccatggcta gcgacttgtt
      541 gtaatctgca agggccaggt cgaatttacc gaggtcttta taggctatgc cgcgttccac
      601 atagattctg ctgttcttgg ggtcaagctc cactgccttt ttgtagtgct gcagggcgcc
      661 ttcagaatct atgactttct ggcattgctt ggctttctga aattcccggg cagcctgatc
      721 aggaccggat gcttgagcag cgtttccggt taatagcatt gaaacaataa tcgccgcccg
      781 tattcgcctc atagaggcta tcatagcgcc tgggaagtgg tagaggcaaa ccctgtacta
      841 tccctgagcg cctgcaccac ttcttcgtct ctggtttgag agaaatcctg gtagtaaagt
      901 ccgaccgcgc aaaagggatc cggcacgctc agggttacga ttgagtcgca gtgagacttg
      961 agctcttcca tgctctcttt actcatcacc ggggcggcca gaatgacctt ggctgcgccc
     1021 tgctttcttg ccgtttctac ggcagctaga gccgtcatgc ctgttgcgat gccatcatcc
     1081 acgattatta cagtttttcc tttgagggag gctttgcggc gtccagccag gcgataaaac
     1141 tgtgcttcca cagcttttgt tttttcgagc aatagcttac tttgttgctc aatatagtca
     1201 tgccacctgg tgcgctcccc ttccgtcatc ttttctcgtc ccggcacatc gtgatttaag
     1261 accacaatgc catcggctga aaccgcaccg atagcaaact ccggttgtcc cggatagggt
     1321 agcttcttgg actcgatgag ttccagcgga ctgctgagtt ttttagctac ttccagtgcc
     1381 accggtacgc cgccgcgcgg taagccgacg acgcaatatg gaatggtatt gcttgcggtg
     1441 aataaggctg ccagcttctc ggctagtttg gcaccggcat cggctctatt tcgaaagatc
     1501 attttggcac ccggtcggca aagg
//
LOCUS       DAOQNZ010001130         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_67012_length_1520_cov_3.308532, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001130 DAOQNZ010000000
VERSION     DAOQNZ010001130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67012_length_1520_cov_3.308532"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /gene="glyQ"
                     /locus_tag="PKW73_17045"
     CDS             complement(<1..772)
                     /gene="glyQ"
                     /locus_tag="PKW73_17045"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNA75061.1"
                     /translation="MQQEFAMALTFQQVIHSLNDFWGRHGCVVMQPFDLEKGASTMSP
                     GTFLRVLGPEPWNMACADPCRRPTDGRYGENPNRLQHYFQYQVILKPSPDNVQEIYLE
                     SLKELGINPLEHDIRFVEDNWESPTLGAWGVGWEVWLDGLEITQFTYFQQAGGLDVRP
                     VAAEITYGVERLSMYLQNVDNVYELQWNDHVKYGELYRANEVQQSTYNFEKSNPEMLS
                     TLFHLAETEALRLLDEKLVLPAYEQILKCSHTFNLLDAR"
     gene            1186..>1520
                     /locus_tag="PKW73_17050"
     CDS             1186..>1520
                     /locus_tag="PKW73_17050"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA75062.1"
                     /translation="MDSQRITGVQDMDPYDGAGPAQRVQVLSPKVQLLLDNINGVLVG
                     QEHATRLSVVAFLAGGHCLIEDVPGVGKTLLAKTLAMSVHAKFKRIQCTPDLLPSDIC
                     GVSIFEAKEG"
BASE COUNT          379 a          406 c          398 g          337 t
ORIGIN      
        1 cccgcgcatc cagcaagtta aaggtatggg agcacttgag aatttgctcg taggccggca
       61 ataccagttt ttcgtcaagg aggcgcagcg cctcagtttc agccagatga aagagggtgc
      121 tgagcatctc gggattgctc ttctcgaaat tataggttga ctgctgcact tcgttggctc
      181 ggtacaactc accgtattta acatggtcgt tccactgcaa ttcatagacg ttatctacgt
      241 tttgcaggta catggagagg cgttccaccc cgtatgtgat ctcggcggca accggtctca
      301 catcaagacc accggcttgt tgaaagtaag tgaattgagt aatttccagt ccatccagcc
      361 agacctccca gccgacgccc caggcgccca gtgtgggcga ttcccagttg tcttccacaa
      421 agcgaatgtc atgttcaagc ggattgatac ccagctcttt caggctttcc aggtagattt
      481 cctgcacatt gtccggagac ggtttgagaa tcacctgata ctgaaagtaa tgctgcaaac
      541 ggttgggatt ctcgccatag cgaccatcag tcggtctgcg acagggatcg gcgcaagcca
      601 tattccacgg ctccggaccg agaactctca gaaaggtacc cggactcatt gtggaggcgc
      661 ccttctcaag gtcgaaaggc tgcatgacca cgcaaccatg gcggccccag aaatcattga
      721 gactgtggat tacttgttga aaggtcaaag ccatggcgaa ttcctgttgc aaaaggtctt
      781 tcttgccccg gcaggggctt cgatgagctg gcgcaagcgc taaagcaggt gccagtttaa
      841 cacctgccac tcgtcaagag aaggaactga ggcagcacat ttacccaact acattttcca
      901 aatagcaagt cttccttaga gtcggcgcag gtccgataag cagcaaccgc gagccctgcc
      961 gcaagcaatc ctggtccaaa ttccaaccgg aaaaattgcg ggcgaagcgt ggaccgtgag
     1021 aatttagtca gtatgtgaat ttagtcagta taatgattat tcaatttttt cctggattca
     1081 cagctcaatg accgcatggg gaattcaaga gaagagagaa tgaagcggct gtgaccacag
     1141 ccatactaag accaacttgg caaccatctc aacaaggagt ccacggtgga tagtcagcgg
     1201 ataacaggcg tacaagacat ggacccctat gacggcgccg ggccagccca gagagtgcag
     1261 gtgctctcgc ctaaggtgca gttgcttctc gacaacatca atggcgtcct ggtcggccag
     1321 gagcacgcca cgcgcctgtc agtggtagcc tttctggccg gcggtcattg cctcatagaa
     1381 gacgtgcccg gagtcggcaa aacacttctg gccaagaccc tggccatgtc ggtgcatgcc
     1441 aaattcaagc gaattcaatg tacgcccgac cttctgcctt cggatatctg cggcgtcagc
     1501 atcttcgaag ccaaggaagg
//
LOCUS       DAOQNZ010001131         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_67261_length_1517_cov_3.992476, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001131 DAOQNZ010000000
VERSION     DAOQNZ010001131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67261_length_1517_cov_3.992476"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKW73_17055"
     CDS             <1..238
                     /locus_tag="PKW73_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75063.1"
                     /translation="LFLSISQGEVIGRVDFAGEGPELLSLVKEHIVSFAAEAARGRFQ
                     IAPIDDKVCLSCDHQRVCRIAELGKEMDLEGGED"
     gene            245..>1517
                     /locus_tag="PKW73_17060"
     CDS             245..>1517
                     /locus_tag="PKW73_17060"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNA75064.1"
                     /translation="MLTSEQIVAVNTVEENVLVSAGAGSGKTHVLVERYVEILRRNEE
                     ISLANIIAVTFTRKAAAEMRSRLKSKFLSLSRDAAEIAGGYSERWRKTMLEVDGARIG
                     TIHSLCESILKAFPGDCGIDPQFLVLDELKTSEMLSSSVEHAIRTVIGAEPGSAAAGA
                     VPLPALDDALLVLNEFSIDELRKLLSGMIRSSMQLKQTLAAVWAACGSPAQADQFSMV
                     PPQASLKAAAQAVMNQVREQASQSLLQDPELKRALLWLSNNPHSDPKNGLEAVRLEVL
                     SQFEEAGRGPAALTWQAFQAIAACGLKAGGNKPESKELREGLKSVRERIKAVVEKLPP
                     SLLPEDERGFACARGLLQLFQIAYEYYGEEKAKLTALDYNDLIDQTIRALSQPQSRAR
                     LYFHERLRALLVDEFQDTNDVQARLLSLMAGP"
BASE COUNT          313 a          388 c          454 g          362 t
ORIGIN      
        1 actcttcctc agtatttctc agggcgaagt gatcgggcgg gtggattttg ccggtgaggg
       61 gcccgagcta ttgtctctag taaaagagca tatcgtgtct tttgccgctg aggctgcccg
      121 ggggcgcttt caaatcgctc ccatagacga caaggtttgc ctcagttgcg atcatcaaag
      181 ggtttgccgg atcgccgagc tgggcaagga aatggatctg gaagggggcg aagactagga
      241 ttctatgctg acttccgagc aaatagtggc ggtcaacacc gtagaagaaa acgtcctggt
      301 ttcggccggt gccggctccg gtaaaaccca tgtgctggtg gagcgctatg ttgagatctt
      361 gcggcgcaat gaagagatca gcctggctaa tattattgcc gttactttta ctcgtaaggc
      421 tgccgctgaa atgcgctcgc gcttgaaaag taaattcttg agtctttccc gtgacgccgc
      481 agagattgcg ggcggctatt cggagcgttg gcgcaagacc atgctggagg tagatggggc
      541 aagaatcggc accattcact ctctttgcga gtctattctg aaggcttttc cgggcgactg
      601 cggcatcgat ccacagtttc tagtgctgga tgagttgaaa accagcgaga tgctgtcctc
      661 ctccgtagag cacgcgattc gcaccgtcat tggtgcagaa ccaggtagtg ccgccgctgg
      721 tgccgtgcct ctgcctgctc ttgatgatgc gctgcttgtg ctgaatgaat tttccatcga
      781 cgaattgcga aaattactct ccggcatgat tcgctcttcc atgcagctga agcagacact
      841 ggctgcggtc tgggcggcct gtggttcgcc ggcacaggcc gaccagttct ctatggtgcc
      901 gccccaggca agcctcaagg ctgcggccca ggcggttatg aatcaggtaa gagagcaggc
      961 ttcgcagtcg ctcttgcagg atccggaact gaagcgtgct cttctctggt tgagtaacaa
     1021 tcctcatagt gatccgaaga acggtctgga agcagtgcgg ctggaagttt tgtcgcagtt
     1081 cgaagaggct ggccggggac cggctgctct tacctggcag gcttttcaag caattgccgc
     1141 ctgtggtttg aaagccggcg gcaataagcc cgagtccaag gaactgcgag aggggctgaa
     1201 gtcggtgcgg gaaagaatca aggccgtggt ggaaaagctt cccccctctc tcttgcctga
     1261 agacgagcgg ggttttgctt gcgcccgcgg tctcctgcaa ctcttccaga ttgcttatga
     1321 atactatggg gaagaaaagg caaagctgac ggcccttgat tataacgacc tgatcgatca
     1381 gacgattcgg gccctttctc aacctcagtc acgtgctcgc ctctactttc atgagcgcct
     1441 gcgggcttta ttggtggacg aattccagga taccaacgat gtgcaggcca ggcttctcag
     1501 tttgatggcc ggacccg
//
LOCUS       DAOQNZ010001132         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_67832_length_1511_cov_3.546016, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001132 DAOQNZ010000000
VERSION     DAOQNZ010001132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67832_length_1511_cov_3.546016"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PKW73_17065"
     CDS             complement(<1..880)
                     /locus_tag="PKW73_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75065.1"
                     /translation="MIKWPTPDPPDPKAKNLRRTAANRPPGKLALLAIRKQRLIQMTR
                     WQNQNHTLAEILRLAALRTITLCTAALSIATSCSMASAQTTEKQRPPKDLKYRKRVIV
                     GPDGGDIPRRIDYQALSTRDTLMLPTYVPAAAARHAIGGFDTGSNTFSRDGIVPPPPP
                     MKSALLPKSIAPLPQEETATARSISPPQRTIAHVPLSQARLVQIKTEAQILFKKGKLA
                     EAQALLSRYVSEYPQEASLKTELAKVSLARAKAHVKQGDHANAAKQARLAVSHSHDVS
                     PEIASAADQALDASLQK"
     gene            1214..>1511
                     /locus_tag="PKW73_17070"
     CDS             1214..>1511
                     /locus_tag="PKW73_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75066.1"
                     /translation="MTAISKEQEQILTERAHSLTRQARLSAAVFSQYSQAQVDRIVKA
                     MTTCAIDNAARLARMAHEETRMGVTEDKVLKNLVASEFLYAQIKDKRTVGVIKEL"
BASE COUNT          297 a          370 c          461 g          383 t
ORIGIN      
        1 gcttttgcaa gctggcatca agggcctggt ctgcggcgct ggcaatttcc gggcttacgt
       61 catggctgtg acttacggcc aggcgggctt gcttggcggc attggcatga tcaccctgtt
      121 tgacgtgagc cttggctcga gcgagagaga ctttggccag ttcggttttc agggatgctt
      181 cctggggata ttcgctcaca taacggctga ggagggcctg ggcttccgcc agtttcccct
      241 tcttaaataa aatctgcgct tcggttttga tctgcaccag ccgtgcctgg ctcagcggca
      301 catgggcgat ggtgcgctgg ggcggtgaaa tggaacgggc cgtggctgtt tcttcctgag
      361 gcaggggggc gatactttta ggcagaaggg ctgatttcat gggcggcggc ggcggcacga
      421 tgccgtcccg gctgaaggta ttgctgccgg tgtcgaagcc gccaatggca tgcctggctg
      481 ctgctgccgg tacataggtg ggcagcatga gggtatccct tgtgctcagg gcttgataat
      541 cgatgcgcct gggaatgtcg ccgccgtcag gcccgacgat tacgcgtttt ctgtatttga
      601 gatctttggg cggacgctgc ttctcagtgg tctgggcgga agccatggag caggaagtgg
      661 cgatggagag tgcggcggtg caaagggtga tggttctcag ggctgcgagt ctgaggattt
      721 cagccagggt gtgattttga ttttgccagc gggtcatttg aattagcctt tgcttgcgta
      781 ttgccaatag cgccaatttg ccgggcggtc tgtttgcggc ggtgcgcctg aggtttttag
      841 ccttggggtc cggcggatca ggggtcggcc atttgatcat gactaattct atgatctgta
      901 tcttgacatg caatggtgat taccacgcct tttgtcaaaa attgacccgc ttgtctacgt
      961 gggattcccc ctgaaagtgc aattgccccg gcgttctcgg ggaatatgcc ggtgtgttgc
     1021 cagaggatta ttagttttat ttagagattg caaaagtcta tattctatat aggaagttgt
     1081 cctacacttg ataattgcct tggggctagc cgccggacaa atttagtcgg gttggttctc
     1141 gtttgacgag ccgggtgagc gcccagcagt tttttattcc tcgctccatc acaaatatcg
     1201 cagaactagt tcaatgacag ccatctctaa agaacaggaa cagatcctca ccgagcgcgc
     1261 ccactctctc accaggcaag caaggttatc ggcggccgta ttcagccagt attcgcaagc
     1321 ccaggttgat cgcatcgtca aagccatgac cacctgcgcc atcgataacg ccgccaggtt
     1381 ggcacgcatg gcccatgaag agacccgcat gggagtcact gaagacaaag tcttgaaaaa
     1441 tctggtggct tccgagtttc tctacgccca gataaaagac aagcgcaccg tgggcgtgat
     1501 caaagaactg c
//
LOCUS       DAOQNZ010001133         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_68201_length_1507_cov_4.048898, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001133 DAOQNZ010000000
VERSION     DAOQNZ010001133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68201_length_1507_cov_4.048898"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PKW73_17075"
     CDS             complement(<1..799)
                     /locus_tag="PKW73_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75067.1"
                     /translation="MDESMRLLVLIAFIALLITIFIFSRLVNRGSKLWRLNDLNDEKL
                     GLLSPLKRHYQLRTDYSGTAIDLPRGQVSKAMKLLSGTIHTLIEKRGQPTEAIATYRA
                     PEADFEILKKIPLECRGDFPVAQWQVKRIVRNTILPFWRRDIVIYGQNGGEPPTTNDG
                     KLHLVLFCSSQKERAQTRPLNIWGLELPRIAQYNSNPHALRFVDPEDKTCGAELIGNN
                     LYITFDVLQFTAEMTERKRFWRKVRTFLGGRDFHLGLLARSLTRITEE"
     gene            complement(876..1211)
                     /locus_tag="PKW73_17080"
     CDS             complement(876..1211)
                     /locus_tag="PKW73_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75068.1"
                     /translation="MAENQNQNTDPKPVVLTKIAPTTNQPADQTAGRLEINPEPVETR
                     ITVNVGVQGKMRKVLLPSGSTVADALRAAEFDAKDHEIRMGGAPARLDTPVEDGRTIM
                     LLRPVRGNN"
BASE COUNT          286 a          357 c          445 g          419 t
ORIGIN      
        1 gctcctccgt tatacgggtc agactgcggg ccagcaaacc caggtggaag tctctgccgc
       61 ccaggaaggt tctcacttta cgccagaagc gcttgcgttc ggtcatttca gctgtaaatt
      121 gcaagacgtc gaaggtgatg tagaggttgt tgcctatgag ttccgcgccg caggttttgt
      181 cctccgggtc gacgaagcgg agcgcatgag gattggagtt gtattgagcg attcgcggca
      241 gctccagacc ccagatgttg agcggccttg tctgggcgcg ctccttttga ctggagcaga
      301 agagcaccag atgcaatttg ccgtcattgg ttgtcggtgg ctcgccgccg ttttgtccgt
      361 aaatgacgat gtctcgacgc cagaaaggca gaatagtgtt gcgcactatt cgtttgacct
      421 gccattgcgc cacgggaaaa tctccccgac actcaagcgg tattttcttg agtatctcga
      481 agtcggcttc cggggctctg taagtggcga ttgcttccgt cggctgccca cgcttctcaa
      541 tgagggtgtg gatggtgccg gagagaagct tcatggcttt gcttacttga ccgcgcggta
      601 gatcgatggc ggtgcctgag taatcggttc taagttgata gtgccttttc agcgggctca
      661 gcaagcccaa cttctcgtcg ttcaggtcgt tcagccgcca cagcttgctg cccctgttga
      721 ccaatcggga gaatataaaa atcgttatca ggagggcaat gaaagcgatc aaaactaaga
      781 gtctcataga ttcgtccata gtatttttct gttttgaaag ttgctcgggg aagcccgcac
      841 tgagtcgacc accctgcagt gcgagctttt agttcttagt tgttgcctct gactgggcgc
      901 agaagcatga tggtgcgtcc gtcttctacc ggtgtgtcga gtcgggctgg ggcgcctccc
      961 atgcggattt cgtggtcttt ggcgtcgaat tcagctgctc tcagagcatc ggccacggtg
     1021 gagccgctgg gcagaagcac ttttctcatc ttgccctgta caccgacatt gactgtgatc
     1081 cgggtttcta caggttccgg atttatttcc agtctgccgg ctgtttgatc ggcaggttga
     1141 ttggttgtcg gtgcaatttt ggtgagaact acgggttttg ggtcagtgtt ttggttttgg
     1201 ttttcggcca tggaaatacc ttgagctgtg ctcgtttggt gaaggtgtgg ggatgtgttt
     1261 tggctgtact tcttcgccgc cggcctgatt tggcaaacat atttgctgca agaggttgac
     1321 tgagtcaggc gtagttgggc tctttagaaa atttagaaag gggtggggga gaagaagata
     1381 cttattgggg ttaacagtta gttcctcaag ttttcaacac catcggtaat ttctttgatg
     1441 caagcaggcc taccctgctc tgccctcttg gaaagcggcg ggtgccgaac ttgcccagtg
     1501 ctgccca
//
LOCUS       DAOQNZ010001134         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_68281_length_1506_cov_4.811854, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001134 DAOQNZ010000000
VERSION     DAOQNZ010001134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68281_length_1506_cov_4.811854"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..>1506
                     /locus_tag="PKW73_17085"
     CDS             53..>1506
                     /locus_tag="PKW73_17085"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNA75069.1"
                     /translation="MNSAGAKIEKAVDFQYLLKEWKSMFSPSTMPRDIMAGITVAMVA
                     LPLNLALAVAAGVDPSIGITTGIVAGFVVALFGGQRYAITGPAAAMAVVLIEIAHTYG
                     LSGIWFVGLIAGLLQIIAGLLRLGRLISYIPMPVIVGFANAIGILVMFNSLGDFFGLP
                     GKVLSNRAEGAHVLGAAPFIPEFIKDVSILFEKVVLHGQANYQALAIGILVIVLAVLV
                     PRFTKVVPGQLVAIVVASLAAYAFHFDVPRIINVAPIPPIDLLPGLPVFPINVGPGEF
                     QSLLLYSMTIFMLGSIESLLSASVADGMTMSSRHRADQELFGQGLANIIMPFFGGIPV
                     TGVIARTAVNISAGAKTRLSAIVHSLVLLLMALTLTRLAEQIPLSALAGILVLTGFRL
                     IEWDEIREIWRGSKTEGWVVVITTLAAVAVDLTAGVVAGLLLTCVLFVRQMSSVKMVT
                     EGDDMDERLSAGQLIPSCKFVRTYLDDGPLFFGAV"
BASE COUNT          260 a          402 c          426 g          418 t
ORIGIN      
        1 ctgtcacaag tgcatcttct ctaccgcaaa tactccagag gttcagtctt ccgtgaatag
       61 cgccggtgca aaaatcgaga aagccgtgga cttccagtat ctgctcaagg agtggaagtc
      121 gatgttctcg ccttccacca tgcctcgcga tatcatggcc ggtatcacgg tggctatggt
      181 ggcgctgcct ctgaacctcg ctctggcagt ggctgccggc gttgatcctt ctattggtat
      241 cacaacaggc atcgtggctg gtttcgtggt ggcccttttc ggcgggcaac gttatgccat
      301 caccggccct gctgcggcaa tggctgtggt cttgattgag attgcccata cctatggctt
      361 gagcggcatc tggtttgtgg gactgattgc cggtcttctg cagattattg ccggactttt
      421 gcgtttggga cgattaatca gctacattcc catgccggtc attgtgggct ttgccaatgc
      481 catcggtatt ctggttatgt tcaactccct cggtgacttt tttggcttgc cgggcaaggt
      541 gctttccaac agggcagaag gcgcccatgt tctgggggcg gcgcccttca ttccggaatt
      601 cataaaagac gtttccatac tttttgagaa agtggtgctg cacggtcagg ccaattatca
      661 agccctggcc atcggtattc tcgtaattgt tctggcggtt ttggtgccca ggtttaccaa
      721 ggtcgtaccg gggcagctgg tggctattgt cgtagcctcc ctggccgcct atgcctttca
      781 tttcgatgtg ccccgcatca ttaatgtcgc acccatcccg cccattgatc tgctgcctgg
      841 tctgccggtt tttcctatca atgtcggacc cggtgagttt cagtcactat tgctttactc
      901 catgaccatc ttcatgctcg gcagtatcga gtctttgctc agcgcctcag tggcggatgg
      961 tatgaccatg tcctcccgtc acagggctga tcaagaactg ttcggtcagg ggctggccaa
     1021 tatcatcatg cctttcttcg gcggcattcc cgttaccggg gttattgcca gaacagcagt
     1081 aaacatcagc gccggtgcta aaacccggct ttcggccata gtgcattccc tggtgctctt
     1141 gctgatggcc ctgacgctga cccggctggc tgagcagatt cctttatcag ccctggccgg
     1201 catcctcgtc ctcaccggtt tccgattaat tgaatgggat gaaatcaggg aaatctggcg
     1261 gggctcgaaa acggaaggct gggtggtggt tatcactacc ctggcggcgg tggcagtgga
     1321 cctgacggcg ggtgtggtgg ctggtttgct cttgacctgt gtgctctttg tcaggcagat
     1381 gagttcggtg aagatggtca ccgagggcga tgacatggat gagcgccttt cggccggtca
     1441 gcttatcccc agttgtaagt ttgtccgaac ctaccttgat gatggaccgc tgttcttcgg
     1501 ggcggt
//
LOCUS       DAOQNZ010001135         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_68283_length_1506_cov_4.740868, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001135 DAOQNZ010000000
VERSION     DAOQNZ010001135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68283_length_1506_cov_4.740868"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKW73_17090"
     CDS             complement(<1..286)
                     /locus_tag="PKW73_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75070.1"
                     /translation="MRKTLTGLLALLFILMPAISFAGQFTDKQLDSLQVLDAKLYTVQ
                     ELQDKQVLSAEQAVVAGKLYTDAAKAIVGHDVTAAELHALVKSEVSTEGLG"
     gene            complement(416..>1506)
                     /locus_tag="PKW73_17095"
     CDS             complement(416..>1506)
                     /locus_tag="PKW73_17095"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNA75071.1"
                     /translation="SYSALACCDAIIEAIPERIDLKLKCYAAIDQAMEEAGREAPYFI
                     FSNTSALSLNSLAQGVKYKERFSGMHFFNPVSRMLGVELPGTEHTSQETVATSIALVG
                     AMGKIALPCKDYPGFIVNAILGVELLVTAHLLSMGVSPEAIDKAMKAFGMPMGPVELM
                     DYVGLDVVLSVAKTLGAAHGERLALPENDLISALVAAGELGRKTGMGFYKWQGEKVLK
                     VAKPARGLVKSRVEKLTGGAKREAVFNPVLRQLAPTLGQYSMSTEAIQEILVGAIESE
                     AVRVLAAGVVDEPFLLDLAFVISTGFTASLGGPLRHIDQRGSKTFFDLMSDIGHGSAA
                     NGQSWRKNFEPCQYLTELAERRGNIYPA"
BASE COUNT          308 a          409 c          447 g          342 t
ORIGIN      
        1 taccaagacc ttctgtacta acctcgcttt tgacgagagc gtgcaattca gccgctgtca
       61 cgtcgtgtcc gacgatggct ttggcggcat cggtgtacag tttgccggcc acaacagctt
      121 gctcggcaga gagcacctgt ttgtcctgta actcctgtac ggtgtaaagc ttggcgtcaa
      181 ggacttgcag tgagtccagt tgtttatcgg tgaactgtcc ggcaaagcta atagccggca
      241 tgaggatgaa gagcaaggct agcaagcctg taagtgtttt tcgcatggaa aatgcctcat
      301 agttgaattt gagggtggga gaaaggcctg gctctttgcc gaaacacctt tctccctcag
      361 cctgtgggtc tttaggtcaa gaccggtggt gttcgcaccg gtctcgggtt agtttttaag
      421 cgggatagat gttgccgcgt ctttcagcaa gctcggtcag gtactgacag ggctcgaagt
      481 ttttgcgcca gctctgaccg tttgccgcac tgccgtgacc gatgtcgctc atcaggtcaa
      541 agaaggtctt gctgccccgt tggtcgatat gacgcaacgg tccgcccaga gaggcagtga
      601 agccggtgct gatcacgaag gccaggtcga gcaggaaggg ctcgtccacg acaccggcgg
      661 caagtacgcg cacagcttcg ctttcgatgg cgcccaccag aatctcctga atggcttccg
      721 tggacatgct gtactgaccc agtgtaggag ccagttgacg cagcacaggg ttgaacaccg
      781 cttcccgttt ggcgccgccg gtcaatttct ctacacgaga tttgaccaat ccgcgagccg
      841 gtttggctac cttcagaacc ttttcaccct gccatttgta gaagcccata ccggtcttgc
      901 gacccagttc accggctgct accagggcag agatcagatc gttttcaggc agcgccagcc
      961 gttctccgtg ggcagcaccc agggttttgg ctacgctcaa gaccacgtcg agaccgacgt
     1021 aatccatcag ttctaccgga cccatgggca tgccgaaagc tttcatggct ttgtcgatgg
     1081 cctcgggaga gacacccatg gaaagcaggt gggcggtgac cagaagttcc acgcccaaaa
     1141 tggcgttgac gatgaagccc gggtagtcct tgcaaggcag ggcgattttg cccatggcgc
     1201 ccacaagagc aatggaagta gccacggttt cctggctggt gtgctcggtg cccggcaact
     1261 ccacacccag catacggctg accgggttga agaagtgcat gccgctgaag cgctctttgt
     1321 atttgacgcc ttgcgccagg ctgttcaaag agagggcgga ggtgttggag aagatgaagt
     1381 acggtgcctc tctgccggct tcttccatgg cctggtcgat ggcggcatag catttcagct
     1441 tgaggtcgat gcgctcggga atggcttcga tgatggcatc gcagcaagcc agcgcactgt
     1501 agctgg
//
LOCUS       DAOQNZ010001136         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_68314_length_1506_cov_2.970365, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001136 DAOQNZ010000000
VERSION     DAOQNZ010001136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68314_length_1506_cov_2.970365"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PKW73_17100"
     CDS             complement(<1..459)
                     /locus_tag="PKW73_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA75072.1"
                     /translation="MKKTKVVKVMAPKKKAAPAKAKAKAKPETAKGKGKIIKGVPAGM
                     TLAKAAKWAAPRISKKRFQHVTGVVEVGLKLAKLAKLSPADRLLVGLACWLHDSCKEM
                     KDYELIEKARAAGMKLTEIEKNNGHLLHGPVAALIVRKELKISNKTVLNAI"
     gene            complement(456..1166)
                     /gene="nadD"
                     /locus_tag="PKW73_17105"
     CDS             complement(456..1166)
                     /gene="nadD"
                     /locus_tag="PKW73_17105"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNA75073.1"
                     /translation="MRELGLFCGTFNPVHMGHLLIAESARVDFKLETVYFVVSPEPPH
                     RSVGLLAGEARYELVKAAVAENPCFQASDLELQRPGPSYTVDTVLSMRDLYPDYRINL
                     IIGSDNLPFLKDWHRAEELVSLVRLLVVPRLRIIGRPSDSAAEADSKSLPAAENALEK
                     QATLDLGLSQQSLCREGLLQDKEVHLVDFPGVGISASGIRKRIGERKSILYMVPASVN
                     RIILQKGYYAKNSKVTDR"
     gene            complement(1175..>1506)
                     /locus_tag="PKW73_17110"
     CDS             complement(1175..>1506)
                     /locus_tag="PKW73_17110"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HNA75074.1"
                     /translation="RDSRLAMIPIGYADGVDRGLSNKMTAFLAGKPIQQVGRISMDQM
                     LFDITDLPEAQEGDVVTLIGEENASQKTGSINLSQWAFLLDTITYELACRLRARLPRI
                     YTRTRGK"
BASE COUNT          290 a          413 c          401 g          402 t
ORIGIN      
        1 aatggcattg aggacggtct tgttggagat tttcagttcc tttctgacaa ttaaagccgc
       61 caccgggccg tgcaagaggt gaccgttgtt tttctctatt tccgtaagct tcatgccggc
      121 tgctctcgct ttttcaatca gctcgtagtc cttcatttct ttacagctgt cgtgcagcca
      181 gcaggcaagg ccaaccagaa ggcgatcggc aggcgagagt ttggccagct tggccagttt
      241 gagtcccact tccaccaccc cggttacatg ctgaaagcgt ttcttggaaa tgcgcggggc
      301 cgcccacttc gcggccttcg ccagggtcat gccggcgggg actcctttga ttattttgcc
      361 tttgcccttg gcggtttccg gttttgcttt ggctttggct ttagccggcg cagccttctt
      421 cttgggcgcc atgactttaa ctactttggt ttttttcatc tgtctgtcac tttgctgttt
      481 ttggcgtagt aacccttctg aagaattatg cggttgaccg acgctggtac catatatagt
      541 atcgatttcc gctcgcctat gcgcttgcga attcccgaag ccgaaattcc tacgccgggg
      601 aagtctacca gatgcacctc tttgtcttgc aataagccct cccggcaaag gctttgctgg
      661 gaaagaccga ggtccagggt ggcttgcttc tctagcgcgt tttcggccgc cggtaggctt
      721 ttcgagtctg cctccgcggc agagtcagag ggccgtccga tgattctcag tctcggtacc
      781 accaggaggc gcaccaggct gaccagttct tccgccctgt gccagtcttt caaaaacggc
      841 aaattgtccg agcctataat caagttgatg cggtagtccg ggtaaaggtc tctcatggag
      901 agcaccgtat caaccgtgta ggacggcccg ggtctttgca gttccaggtc gctggcttga
      961 aaacaaggat tttccgctac tgctgctttt accagctcat agcgagcttc tccggccagg
     1021 agccctacgc tgcggtgggg cggctccgga ctgactacaa agtaaaccgt ctccagtttg
     1081 aaatccaccc gcgccgattc ggcaatgagc agatgcccca tatgcacggg gttgaaagtt
     1141 ccgcaaaaaa gtccaagttc tcgcaagggg cctcctactt gccccttgtc ctcgtataga
     1201 tacggggtag tcgcgctctc aggcggcagg caagttcgta agtaatggta tcaaggagaa
     1261 aagcccactg gcttaagtta attgaacctg ttttttgaga ggcgttctct tccccgatga
     1321 gagtgaccac gtctccttcc tgagcttccg gcaggtcggt tatatcgaag agcatctggt
     1381 ccatgctgat gcgacccact tgttgaatgg gttttccggc taagaaagcc gtcattttat
     1441 tgctcagacc ccggtccaca ccgtcggcat agccgatggg aatcatggcc agacgggaat
     1501 ctctgg
//
LOCUS       DAOQNZ010001137         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Obscuribacter sp. isolate AMR_MDS_0483
            NODE_68847_length_1500_cov_3.490657, whole genome shotgun sequence.
ACCESSION   DAOQNZ010001137 DAOQNZ010000000
VERSION     DAOQNZ010001137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810115
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Obscuribacter sp. (wastewater metagenome)
  ORGANISM  Candidatus Obscuribacter sp.
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Obscuribacterales; Candidatus Obscuribacteraceae; Candidatus
            Obscuribacter.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:38:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,424
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,388
            CDSs (with protein)               :: 3,388
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Obscuribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68847_length_1500_cov_3.490657"
                     /isolate="AMR_MDS_0483"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2711154"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PKW73_17115"
     CDS             complement(<1..609)
                     /locus_tag="PKW73_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75075.1"
                     /translation="MSLSHKPGAAFGAKLAELRVELLAFLLFSLAALLAASFYVGGER
                     FFYISDFNFYHYACISTWELWATKAWIVPLFLYFSTKMTHNLLFTALPSLLMVLGGPG
                     RVSYIVSLMASFTLPYLLALALVLRQTVPSEQKRTRTVLWLMTLLAVALLPVFFVPVL
                     RGYPDNLAALPSIIAVFLYLKAERKLSTRLCVKIGLLLAFAPL"
     gene            complement(690..>1500)
                     /locus_tag="PKW73_17120"
     CDS             complement(690..>1500)
                     /locus_tag="PKW73_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA75076.1"
                     /translation="GMEAQFSLGFLLDDLDRGLPYVSPGGDARKRLKASRLIQGHVLK
                     LTRSVMPGRLTPPRMLSIARVSVADGDLQAAEAQLVEFMKTLPANSPFRPDFVGSLAA
                     CRARQGKPEAISALLKSLEKKGGKIYARRELAKYYYWSADYPAAFKVLDEAVTLLGPR
                     PDVGQLEELLASKLKLSQDSGNVKMSEEICNKRIHNLRPCRKQYPDLYRTAVTDLYSL
                     VKVVRPADAARVLASSGLDADEIMKPQAEMFITERDRLEMEKAKGLKTGKP"
BASE COUNT          390 a          421 c          396 g          293 t
ORIGIN      
        1 caggggcgca aacgctaaga gtagaccaat tttcacgcaa agtcgggtgg aaagcttgcg
       61 ctcagccttc aaatagagga agacggcaat aatggaaggt agggccgcca gattgtcggg
      121 atagccacgc agcacgggca cgaagaagac cggcagcaat gccaccgcca agagtgtcat
      181 gagccagagc acggtgcgag ttcgcttctg ttccgatggc acggtctgac gcaataccag
      241 cgccagagcc aacagatagg gcaaggtaaa actggccatg agggagacga tatacgacac
      301 ccgaccgggt ccgcccagga ccatcaaaag agacggcaaa gccgtaaaca gaagattgtg
      361 ggtcatcttg gtactgaagt acaagaagag cggcactatc caggccttgg tagcccacaa
      421 ctcccaggtg gaaatacagg cgtagtggta aaaattgaag tcactgatat agaagaaccg
      481 ttcgccgccc acatagaatg aggccgctag cagagccgcc agagagaaaa gcaggaaggc
      541 cagaagttcg actcgcagtt cagccaattt cgcgccgaaa gcagcacctg gcttatggga
      601 tagactcaaa acaaccgctc actctcagac agtactatca actcttcaaa tcggtccgga
      661 aactatcata tcagtcttgt cagccgccct cagggcttgc cggttttcag cccctttgcc
      721 ttctccattt ccaggcggtc acgctctgta ataaacattt ccgcctgggg cttcatgatt
      781 tcatcggcat caagcccgga actagccaga acgcgggccg catctgccgg tctcaccact
      841 ttcaccagtg agtagagatc tgttaccgcc gttctgtata gatcaggata ttgcttacga
      901 cagggtctta aattatgtat gcgcttattg caaatctcct cggacatttt gacattaccg
      961 ctgtcctggc tcaacttcag cttcgaggcc agtagttcct ccaactgtcc gacgtcaggt
     1021 cttggtccga gcaaagtcac ggcctcatcc agaactttaa aggcagccgg gtaatcagcg
     1081 ctccagtaat aatacttggc cagttcgcgg cgggcgtaga ttttgccgcc cttcttctcc
     1141 agagacttga gcagagctga aatcgcttcc ggcttcccct gacgagcccg gcaggcagcc
     1201 agactaccca caaaatcagg acggaaaggc gagtttgccg gcagcgtctt cataaattcc
     1261 acaagctggg cttccgccgc ctgcagatca ccatcggcaa cagagactcg cgcaatactg
     1321 agcatgcggg gcggcgtgag ccgaccgggc attacggaac gggtcagctt gagcacatgg
     1381 ccctgaatca agcgggaagc cttgaggcgc ttccgggcgt caccgccggg acttacataa
     1441 ggtaaacccc ggtctaaatc atccagcaaa aaaccgaggc tgaattgcgc ttccatgccg
//
